LOCUS       DAJOWB010000001       123970 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJOWB010000001 DAJOWB010000000
VERSION     DAJOWB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 123970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 123970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..123970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.001"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(656..1324)
                     /locus_tag="OHO85_000001"
                     /pseudo
     CDS             complement(656..1324)
                     /locus_tag="OHO85_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1447..1899)
                     /gene="tabA"
                     /locus_tag="OHO85_000002"
     CDS             complement(1447..1899)
                     /gene="tabA"
                     /locus_tag="OHO85_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCQ2669439.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTPETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLRGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKVVVKMLMA"
     gene            complement(2044..2637)
                     /locus_tag="OHO85_000003"
     CDS             complement(2044..2637)
                     /locus_tag="OHO85_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCQ2669440.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKVGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            2708..3421
                     /gene="bdcA"
                     /locus_tag="OHO85_000004"
     CDS             2708..3421
                     /gene="bdcA"
                     /locus_tag="OHO85_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCQ2669441.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3552..3947
                     /locus_tag="OHO85_000005"
     CDS             3552..3947
                     /locus_tag="OHO85_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCQ2669442.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            4228..4362
                     /gene="pyrL"
                     /locus_tag="OHO85_000006"
     CDS             4228..4362
                     /gene="pyrL"
                     /locus_tag="OHO85_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCQ2669443.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            4366..5301
                     /gene="pyrB"
                     /locus_tag="OHO85_000007"
     CDS             4366..5301
                     /gene="pyrB"
                     /locus_tag="OHO85_000007"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCQ2669444.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            5314..5775
                     /gene="pyrI"
                     /locus_tag="OHO85_000008"
     CDS             5314..5775
                     /gene="pyrI"
                     /locus_tag="OHO85_000008"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCQ2669445.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5848..6234
                     /gene="ridA"
                     /locus_tag="OHO85_000009"
     CDS             5848..6234
                     /gene="ridA"
                     /locus_tag="OHO85_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCQ2669446.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(6440..9136)
                     /gene="mgtA"
                     /locus_tag="OHO85_000010"
     CDS             complement(6440..9136)
                     /gene="mgtA"
                     /locus_tag="OHO85_000010"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCQ2669447.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(9277..9330)
                     /gene="mgtL"
                     /locus_tag="OHO85_000011"
     CDS             complement(9277..9330)
                     /gene="mgtL"
                     /locus_tag="OHO85_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCQ2669448.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            9515..10462
                     /gene="treR"
                     /locus_tag="OHO85_000012"
     CDS             9515..10462
                     /gene="treR"
                     /locus_tag="OHO85_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCQ2669449.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGILKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            10581..12002
                     /gene="treB"
                     /locus_tag="OHO85_000013"
     CDS             10581..12002
                     /gene="treB"
                     /locus_tag="OHO85_000013"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCQ2669450.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            12052..13707
                     /gene="treC"
                     /locus_tag="OHO85_000014"
     CDS             12052..13707
                     /gene="treC"
                     /locus_tag="OHO85_000014"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCQ2669451.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALAGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            14101..16239
                     /gene="nrdD"
                     /locus_tag="OHO85_000015"
     CDS             14101..16239
                     /gene="nrdD"
                     /locus_tag="OHO85_000015"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCQ2669452.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWSIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            16397..16861
                     /gene="nrdG"
                     /locus_tag="OHO85_000016"
     CDS             16397..16861
                     /gene="nrdG"
                     /locus_tag="OHO85_000016"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCQ2669453.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPDKDIW
                     VWTGYKLDDLNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(16906..17292)
                     /gene="cybC"
                     /locus_tag="OHO85_000017"
     CDS             complement(16906..17292)
                     /gene="cybC"
                     /locus_tag="OHO85_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCQ2669454.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDTLKVVEKADN
                     AAQVKDSLTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(17475..18827)
                     /gene="pmbA"
                     /locus_tag="OHO85_000018"
     CDS             complement(17475..18827)
                     /gene="pmbA"
                     /locus_tag="OHO85_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCQ2669455.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18922..19473
                     /locus_tag="OHO85_000019"
     CDS             18922..19473
                     /locus_tag="OHO85_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCQ2669456.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(19629..21002)
                     /gene="mpl"
                     /locus_tag="OHO85_000020"
     CDS             complement(19629..21002)
                     /gene="mpl"
                     /locus_tag="OHO85_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCQ2669457.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            21178..22176
                     /gene="fbp"
                     /locus_tag="OHO85_000021"
     CDS             21178..22176
                     /gene="fbp"
                     /locus_tag="OHO85_000021"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCQ2669458.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(22209..23204)
                     /gene="yjfF"
                     /locus_tag="OHO85_000022"
     CDS             complement(22209..23204)
                     /gene="yjfF"
                     /locus_tag="OHO85_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCQ2669459.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(23191..24213)
                     /locus_tag="OHO85_000023"
     CDS             complement(23191..24213)
                     /locus_tag="OHO85_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2669460.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATMAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(24227..25729)
                     /locus_tag="OHO85_000024"
     CDS             complement(24227..25729)
                     /locus_tag="OHO85_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCQ2669461.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFNLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(25869..26825)
                     /gene="ytfQ"
                     /locus_tag="OHO85_000025"
     CDS             complement(25869..26825)
                     /gene="ytfQ"
                     /locus_tag="OHO85_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCQ2669462.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            27135..27665
                     /gene="ppa"
                     /locus_tag="OHO85_000026"
     CDS             27135..27665
                     /gene="ppa"
                     /locus_tag="OHO85_000026"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCQ2669463.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(27744..28094)
                     /gene="chpB"
                     /locus_tag="OHO85_000027"
     CDS             complement(27744..28094)
                     /gene="chpB"
                     /locus_tag="OHO85_000027"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HCQ2669464.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAAGE
                     VVEEALLRLQAVVE"
     gene            complement(28088..28339)
                     /gene="chpS"
                     /locus_tag="OHO85_000028"
     CDS             complement(28088..28339)
                     /gene="chpS"
                     /locus_tag="OHO85_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HCQ2669465.1"
                     /translation="MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            complement(28551..28892)
                     /locus_tag="OHO85_000029"
     CDS             complement(28551..28892)
                     /locus_tag="OHO85_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCQ2669466.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(28895..32674)
                     /gene="tamB"
                     /locus_tag="OHO85_000030"
     CDS             complement(28895..32674)
                     /gene="tamB"
                     /locus_tag="OHO85_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCQ2669467.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSWQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(32671..34404)
                     /gene="tamA"
                     /locus_tag="OHO85_000031"
     CDS             complement(32671..34404)
                     /gene="tamA"
                     /locus_tag="OHO85_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCQ2669468.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKMLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            34610..35248
                     /gene="msrA"
                     /locus_tag="OHO85_000032"
     CDS             34610..35248
                     /gene="msrA"
                     /locus_tag="OHO85_000032"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCQ2669469.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            35571..36914
                     /locus_tag="OHO85_000033"
     CDS             35571..36914
                     /locus_tag="OHO85_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCQ2669470.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(36976..37182)
                     /locus_tag="OHO85_000034"
     CDS             complement(36976..37182)
                     /locus_tag="OHO85_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCQ2669471.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            37507..38064
                     /locus_tag="OHO85_000035"
     CDS             37507..38064
                     /locus_tag="OHO85_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCQ2669472.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(38054..38794)
                     /gene="cysQ"
                     /locus_tag="OHO85_000036"
     CDS             complement(38054..38794)
                     /gene="cysQ"
                     /locus_tag="OHO85_000036"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCQ2669473.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            38984..40927
                     /gene="cpdB"
                     /locus_tag="OHO85_000037"
     CDS             38984..40927
                     /gene="cpdB"
                     /locus_tag="OHO85_000037"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCQ2669474.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(41056..41436)
                     /locus_tag="OHO85_000038"
     CDS             complement(41056..41436)
                     /locus_tag="OHO85_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCQ2669475.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            41525..42385
                     /gene="qorB"
                     /locus_tag="OHO85_000039"
     CDS             41525..42385
                     /gene="qorB"
                     /locus_tag="OHO85_000039"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCQ2669476.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            42493..43458
                     /locus_tag="OHO85_000040"
     CDS             42493..43458
                     /locus_tag="OHO85_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCQ2669477.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            43566..44228
                     /gene="ytfE"
                     /locus_tag="OHO85_000041"
     CDS             43566..44228
                     /gene="ytfE"
                     /locus_tag="OHO85_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCQ2669478.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLSRAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(44475..45542)
                     /locus_tag="OHO85_000042"
     CDS             complement(44475..45542)
                     /locus_tag="OHO85_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2669479.1"
                     /translation="MAILHPQECWLLERIMSPEYYRRRFEGWQEFVELCERQVAEWSK
                     TMPLDVRRRPLCEQIDVVWGGRVLPNIRSTLKSVQYDFIQLQQGDLRVLQSGGNISSD
                     MKGLIDYPSDWMSLAAQKQYDRLKWRGAHYNNLIRRTSGGYWYDGELTYYYEESLHGP
                     LALPMQLPLYELDSSVYLREDDSVTVAGLYLPDIPDASAQLLYRSEHIPEAWQGRVRT
                     KYVNEAGIQEYYWESGAWAKCNWKRIRRVANRFINVPPEGFFPQGMPEELYNWPQREA
                     QYVTDRQRIAAFSGEACPHSGEWSIFVEGRQATVTLEQGEQMPEWTDRKMEGEYKRGE
                     KFHVLWSLMNRHDGGSVWVEA"
     gene            complement(45641..46075)
                     /locus_tag="OHO85_000043"
     CDS             complement(45641..46075)
                     /locus_tag="OHO85_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2669480.1"
                     /translation="MAKHSWLLIAALPLSISPSWGADFYYRQQEKGTVYVAEQKGEKD
                     EILSELPGVNFSRLWRIANLANNQETRLLSDFNPDKFDCDDRNNCQHTWLTDGRSVLW
                     SGKVLKNPPGEPNVDAASFQAFGAFAADKRSVYFDGQRTDDR"
     gene            complement(46342..47745)
                     /gene="cycA"
                     /locus_tag="OHO85_000044"
                     /gene_synonym="dagA"
     CDS             complement(46342..47745)
                     /gene="cycA"
                     /locus_tag="OHO85_000044"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCQ2669481.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRMAK"
     gene            complement(48054..48674)
                     /gene="fklB"
                     /locus_tag="OHO85_000045"
     CDS             complement(48054..48674)
                     /gene="fklB"
                     /locus_tag="OHO85_000045"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCQ2669482.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            48893..49531
                     /gene="ytfB"
                     /locus_tag="OHO85_000046"
     CDS             48893..49531
                     /gene="ytfB"
                     /locus_tag="OHO85_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HCQ2669483.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(49515..50162)
                     /locus_tag="OHO85_000047"
     CDS             complement(49515..50162)
                     /locus_tag="OHO85_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCQ2669484.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNNIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            50375..51166
                     /locus_tag="OHO85_000048"
     CDS             50375..51166
                     /locus_tag="OHO85_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCQ2669485.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDLPDADSVE
                     KLHITQQECIAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            51176..52018
                     /locus_tag="OHO85_000049"
     CDS             51176..52018
                     /locus_tag="OHO85_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCQ2669486.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIAHQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            52028..52804
                     /locus_tag="OHO85_000050"
     CDS             52028..52804
                     /locus_tag="OHO85_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HCQ2669487.1"
                     /translation="MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEIEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            52814..54355
                     /locus_tag="OHO85_000051"
     CDS             52814..54355
                     /locus_tag="OHO85_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCQ2669488.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            54352..56433
                     /locus_tag="OHO85_000052"
     CDS             54352..56433
                     /locus_tag="OHO85_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HCQ2669489.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            56454..56930
                     /locus_tag="OHO85_000053"
     CDS             56454..56930
                     /locus_tag="OHO85_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCQ2669490.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSHSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            56973..58274
                     /locus_tag="OHO85_000054"
     CDS             56973..58274
                     /locus_tag="OHO85_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCQ2669491.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIILIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFL
                     ESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            58327..59298
                     /locus_tag="OHO85_000055"
     CDS             58327..59298
                     /locus_tag="OHO85_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HCQ2669492.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(59410..59859)
                     /gene="rplI"
                     /locus_tag="OHO85_000056"
     CDS             complement(59410..59859)
                     /gene="rplI"
                     /locus_tag="OHO85_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCQ2669493.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(59901..60128)
                     /gene="rpsR"
                     /locus_tag="OHO85_000057"
     CDS             complement(59901..60128)
                     /gene="rpsR"
                     /locus_tag="OHO85_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCQ2669494.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(60133..60447)
                     /gene="priB"
                     /locus_tag="OHO85_000058"
     CDS             complement(60133..60447)
                     /gene="priB"
                     /locus_tag="OHO85_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCQ2669495.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQVITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(60454..60849)
                     /gene="rpsF"
                     /locus_tag="OHO85_000059"
     CDS             complement(60454..60849)
                     /gene="rpsF"
                     /locus_tag="OHO85_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCQ2669496.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            61176..61451
                     /locus_tag="OHO85_000060"
     CDS             61176..61451
                     /locus_tag="OHO85_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCQ2669497.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(61580..62266)
                     /gene="ulaF"
                     /locus_tag="OHO85_000061"
     CDS             complement(61580..62266)
                     /gene="ulaF"
                     /locus_tag="OHO85_000061"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCQ2669498.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGKYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(62266..63120)
                     /gene="ulaE"
                     /locus_tag="OHO85_000062"
     CDS             complement(62266..63120)
                     /gene="ulaE"
                     /locus_tag="OHO85_000062"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCQ2669499.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(63130..63780)
                     /gene="ulaD"
                     /locus_tag="OHO85_000063"
     CDS             complement(63130..63780)
                     /gene="ulaD"
                     /locus_tag="OHO85_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCQ2669500.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(63794..64258)
                     /gene="ulaC"
                     /locus_tag="OHO85_000064"
     CDS             complement(63794..64258)
                     /gene="ulaC"
                     /locus_tag="OHO85_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCQ2669501.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(64268..64573)
                     /gene="ulaB"
                     /locus_tag="OHO85_000065"
     CDS             complement(64268..64573)
                     /gene="ulaB"
                     /locus_tag="OHO85_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCQ2669502.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(64589..65986)
                     /gene="ulaA"
                     /locus_tag="OHO85_000066"
     CDS             complement(64589..65986)
                     /gene="ulaA"
                     /locus_tag="OHO85_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCQ2669503.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNIMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            66341..67405
                     /gene="ulaG"
                     /locus_tag="OHO85_000067"
     CDS             66341..67405
                     /gene="ulaG"
                     /locus_tag="OHO85_000067"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCQ2669504.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            67513..68268
                     /gene="ulaR"
                     /locus_tag="OHO85_000068"
     CDS             67513..68268
                     /gene="ulaR"
                     /locus_tag="OHO85_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCQ2669505.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(68265..69014)
                     /gene="yjfP"
                     /locus_tag="OHO85_000069"
     CDS             complement(68265..69014)
                     /gene="yjfP"
                     /locus_tag="OHO85_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCQ2669506.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGAGYFTSLARSLFPPLIPETTA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            69196..69525
                     /gene="bsmA"
                     /locus_tag="OHO85_000070"
     CDS             69196..69525
                     /gene="bsmA"
                     /locus_tag="OHO85_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCQ2669507.1"
                     /translation="MFSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            69674..69949
                     /gene="yjfN"
                     /locus_tag="OHO85_000071"
     CDS             69674..69949
                     /gene="yjfN"
                     /locus_tag="OHO85_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCQ2669508.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(70066..71691)
                     /locus_tag="OHO85_000072"
     CDS             complement(70066..71691)
                     /locus_tag="OHO85_000072"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCQ2669509.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELRREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(71775..72938)
                     /locus_tag="OHO85_000073"
     CDS             complement(71775..72938)
                     /locus_tag="OHO85_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCQ2669510.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(72941..73579)
                     /locus_tag="OHO85_000074"
     CDS             complement(72941..73579)
                     /locus_tag="OHO85_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCQ2669511.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESSDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVISGFLLSRVIRKDRDEQFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(73589..73987)
                     /locus_tag="OHO85_000075"
     CDS             complement(73589..73987)
                     /locus_tag="OHO85_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCQ2669512.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(74005..74664)
                     /locus_tag="OHO85_000076"
     CDS             complement(74005..74664)
                     /locus_tag="OHO85_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HCQ2669513.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(74715..75413)
                     /locus_tag="OHO85_000077"
     CDS             complement(74715..75413)
                     /locus_tag="OHO85_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HCQ2669514.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(75432..75833)
                     /locus_tag="OHO85_000078"
     CDS             complement(75432..75833)
                     /locus_tag="OHO85_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HCQ2669515.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(75960..76691)
                     /gene="rlmB"
                     /locus_tag="OHO85_000079"
     CDS             complement(75960..76691)
                     /gene="rlmB"
                     /locus_tag="OHO85_000079"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCQ2669516.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(76871..79312)
                     /gene="rnr"
                     /locus_tag="OHO85_000080"
     CDS             complement(76871..79312)
                     /gene="rnr"
                     /locus_tag="OHO85_000080"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCQ2669517.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(79351..79776)
                     /gene="nsrR"
                     /locus_tag="OHO85_000081"
     CDS             complement(79351..79776)
                     /gene="nsrR"
                     /locus_tag="OHO85_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCQ2669518.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKSASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(79981..81279)
                     /gene="purA"
                     /locus_tag="OHO85_000082"
     CDS             complement(79981..81279)
                     /gene="purA"
                     /locus_tag="OHO85_000082"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCQ2669519.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(81383..81580)
                     /locus_tag="OHO85_000083"
     CDS             complement(81383..81580)
                     /locus_tag="OHO85_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCQ2669520.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(81662..82666)
                     /gene="hflC"
                     /locus_tag="OHO85_000084"
     CDS             complement(81662..82666)
                     /gene="hflC"
                     /locus_tag="OHO85_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCQ2669521.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(82669..83928)
                     /gene="hflK"
                     /locus_tag="OHO85_000085"
     CDS             complement(82669..83928)
                     /gene="hflK"
                     /locus_tag="OHO85_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCQ2669522.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(84014..85294)
                     /gene="hflX"
                     /locus_tag="OHO85_000086"
     CDS             complement(84014..85294)
                     /gene="hflX"
                     /locus_tag="OHO85_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCQ2669523.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(85371..85679)
                     /gene="hfq"
                     /locus_tag="OHO85_000087"
     CDS             complement(85371..85679)
                     /gene="hfq"
                     /locus_tag="OHO85_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCQ2669524.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(85765..86715)
                     /gene="miaA"
                     /locus_tag="OHO85_000088"
     CDS             complement(85765..86715)
                     /gene="miaA"
                     /locus_tag="OHO85_000088"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCQ2669525.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(86708..88555)
                     /gene="mutL"
                     /locus_tag="OHO85_000089"
     CDS             complement(86708..88555)
                     /gene="mutL"
                     /locus_tag="OHO85_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCQ2669526.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(88565..89902)
                     /gene="amiB"
                     /locus_tag="OHO85_000090"
     CDS             complement(88565..89902)
                     /gene="amiB"
                     /locus_tag="OHO85_000090"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCQ2669527.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(89921..90382)
                     /gene="tsaE"
                     /locus_tag="OHO85_000091"
     CDS             complement(89921..90382)
                     /gene="tsaE"
                     /locus_tag="OHO85_000091"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCQ2669528.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(90354..91901)
                     /gene="nnr"
                     /locus_tag="OHO85_000092"
     CDS             complement(90354..91901)
                     /gene="nnr"
                     /locus_tag="OHO85_000092"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCQ2669529.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            91900..93039
                     /gene="queG"
                     /locus_tag="OHO85_000093"
     CDS             91900..93039
                     /gene="queG"
                     /locus_tag="OHO85_000093"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCQ2669530.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(93022..93075)
                     /locus_tag="OHO85_000094"
     CDS             complement(93022..93075)
                     /locus_tag="OHO85_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2669531.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(93309..93384)
                     /locus_tag="OHO85_000095"
     tRNA            complement(93309..93384)
                     /locus_tag="OHO85_000095"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(93349..93351),aa:Gly,seq:gcc)
     gene            complement(93420..93495)
                     /locus_tag="OHO85_000096"
     tRNA            complement(93420..93495)
                     /locus_tag="OHO85_000096"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(93460..93462),aa:Gly,seq:gcc)
     gene            complement(93706..94251)
                     /gene="orn"
                     /locus_tag="OHO85_000097"
     CDS             complement(93706..94251)
                     /gene="orn"
                     /locus_tag="OHO85_000097"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCQ2669532.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            94346..95398
                     /gene="rsgA"
                     /locus_tag="OHO85_000098"
     CDS             94346..95398
                     /gene="rsgA"
                     /locus_tag="OHO85_000098"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCQ2669533.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            95495..96463
                     /gene="psd"
                     /locus_tag="OHO85_000099"
     CDS             95495..96463
                     /gene="psd"
                     /locus_tag="OHO85_000099"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCQ2669534.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            96485..99808
                     /gene="mscM"
                     /locus_tag="OHO85_000100"
     CDS             96485..99808
                     /gene="mscM"
                     /locus_tag="OHO85_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCQ2669535.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(99959..101461)
                     /gene="yjeM"
                     /locus_tag="OHO85_000101"
     CDS             complement(99959..101461)
                     /gene="yjeM"
                     /locus_tag="OHO85_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HCQ2669536.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGMTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTIFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(101680..102657)
                     /gene="epmA"
                     /locus_tag="OHO85_000102"
     CDS             complement(101680..102657)
                     /gene="epmA"
                     /locus_tag="OHO85_000102"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCQ2669537.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIDALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            102982..104790
                     /gene="frdA"
                     /locus_tag="OHO85_000103"
     CDS             102982..104790
                     /gene="frdA"
                     /locus_tag="OHO85_000103"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCQ2669538.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            104783..105517
                     /gene="frdB"
                     /locus_tag="OHO85_000104"
     CDS             104783..105517
                     /gene="frdB"
                     /locus_tag="OHO85_000104"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCQ2669539.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            105528..105923
                     /gene="frdC"
                     /locus_tag="OHO85_000105"
     CDS             105528..105923
                     /gene="frdC"
                     /locus_tag="OHO85_000105"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCQ2669540.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            105934..106293
                     /gene="frdD"
                     /locus_tag="OHO85_000106"
     CDS             105934..106293
                     /gene="frdD"
                     /locus_tag="OHO85_000106"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCQ2669541.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            106356..107489
                     /locus_tag="OHO85_000107"
     CDS             106356..107489
                     /locus_tag="OHO85_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCQ2669542.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            107578..108111
                     /gene="blc"
                     /locus_tag="OHO85_000108"
     CDS             107578..108111
                     /gene="blc"
                     /locus_tag="OHO85_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCQ2669543.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(108108..108425)
                     /gene="sugE"
                     /locus_tag="OHO85_000109"
     CDS             complement(108108..108425)
                     /gene="sugE"
                     /locus_tag="OHO85_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCQ2669544.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(108600..108746)
                     /gene="ecnB"
                     /locus_tag="OHO85_000110"
     CDS             complement(108600..108746)
                     /gene="ecnB"
                     /locus_tag="OHO85_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCQ2669545.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(108857..108982)
                     /gene="ecnA"
                     /locus_tag="OHO85_000111"
     CDS             complement(108857..108982)
                     /gene="ecnA"
                     /locus_tag="OHO85_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCQ2669546.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(109034..109600)
                     /gene="efp"
                     /locus_tag="OHO85_000112"
     CDS             complement(109034..109600)
                     /gene="efp"
                     /locus_tag="OHO85_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCQ2669547.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            109642..110670
                     /gene="epmB"
                     /locus_tag="OHO85_000113"
     CDS             109642..110670
                     /gene="epmB"
                     /locus_tag="OHO85_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCQ2669548.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            111065..111934
                     /locus_tag="OHO85_000114"
     CDS             111065..111934
                     /locus_tag="OHO85_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCQ2669549.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGNKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLVGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(112127..112480)
                     /locus_tag="OHO85_000115"
     CDS             complement(112127..112480)
                     /locus_tag="OHO85_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCQ2669550.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(112618..114264)
                     /gene="groL"
                     /locus_tag="OHO85_000116"
     CDS             complement(112618..114264)
                     /gene="groL"
                     /locus_tag="OHO85_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCQ2669551.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(114308..114601)
                     /locus_tag="OHO85_000117"
     CDS             complement(114308..114601)
                     /locus_tag="OHO85_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCQ2669552.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            114877..116133
                     /gene="yjeH"
                     /locus_tag="OHO85_000118"
     CDS             114877..116133
                     /gene="yjeH"
                     /locus_tag="OHO85_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCQ2669553.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            complement(116149..116625)
                     /gene="fxsA"
                     /locus_tag="OHO85_000119"
     CDS             complement(116149..116625)
                     /gene="fxsA"
                     /locus_tag="OHO85_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCQ2669554.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFALMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            116962..118398
                     /gene="aspA"
                     /locus_tag="OHO85_000120"
     CDS             116962..118398
                     /gene="aspA"
                     /locus_tag="OHO85_000120"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCQ2669555.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            118516..119817
                     /gene="dcuA"
                     /locus_tag="OHO85_000121"
     CDS             118516..119817
                     /gene="dcuA"
                     /locus_tag="OHO85_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCQ2669556.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFLLGSFML"
     gene            119933..120271
                     /gene="cutA"
                     /locus_tag="OHO85_000122"
     CDS             119933..120271
                     /gene="cutA"
                     /locus_tag="OHO85_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCQ2669557.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            120247..121944
                     /gene="dsbD"
                     /locus_tag="OHO85_000123"
     CDS             120247..121944
                     /gene="dsbD"
                     /locus_tag="OHO85_000123"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCQ2669558.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            121981..122556
                     /locus_tag="OHO85_000124"
     CDS             121981..122556
                     /locus_tag="OHO85_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCQ2669559.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            122663..122738
                     /locus_tag="OHO85_000125"
     tRNA            122663..122738
                     /locus_tag="OHO85_000125"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:122696..122698,aa:Phe,seq:gaa)
     gene            122936..>123970
                     /locus_tag="OHO85_000126"
     CDS             122936..>123970
                     /locus_tag="OHO85_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCQ2669560.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLSTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRISS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRA"
BASE COUNT        30253 a        32941 c        30819 g        29957 t
ORIGIN      
        1 tctgctcgcc ctcataactc ttaagtaatt gatagaagta ccacttagca aaatcaggtt
       61 gcttcatgaa aaaatccttc aaaaaaatat cattcactaa aatgctgaga ggcactttat
      121 caaaaaccaa tttaacatca tcaaaaagcc ttagtattaa gcgcgatttt tcttgattac
      181 ttaaattaaa atcattgaca tatacaacca acttctccca aatcaattcc tgtaactcag
      241 ggctgttttt atagggttct ttattgaggt tgaaaaataa tgtctcagct attttagact
      301 gatcgcagtt aaaatcaata tgatcgatca cggagcgggc atgctttatc tttgtagtat
      361 catcagaata ttgcatactt tgattaaaac accactccag aatttttaca tcattaatat
      421 atggcaattt aaaaaaagtc tccaacatcg aatcgatgac cttttcgaga ttaatttttg
      481 catcatctaa ggaatcaata agaatattag acaggttaat tttgatgtta ttaatttcat
      541 cattaccttc tgtaccacta tcaattttgg tatcatgtaa cgttttcaga ccatttattt
      601 cttcaagtat accaccttcg cctagtaagt atggaatcgt gtcatcacat acatgaggca
      661 ttaatagcag atgttctttc tttatttttt ctaatgtact ggctaatggc ttttctaata
      721 actcgttata atcaattgcg tccttccagt tcaactgatg attatttaat agaagcgcca
      781 cttccttgtt agcttcacgt tgataacacg ccagaacctt gttagtaatc gggtgtaatg
      841 aactctcatc ttttctttca aaggactttc cttcataacg attagtcgaa ccatcaggtt
      901 gttgaaatat aatagccgta tgtccttgac gagtccccat agtagtgata gtggaaagtg
      961 taatttctat ttccacctta gataaatccg ctcgctcccc ttcaggatta ttcataacaa
     1021 acttcattat ctcactgcca tcattatttt tgatataaga tgaggactca atatgagcca
     1081 tcatatttct tcccgcttcg ttggagcggt tgctaaaatc cacataaccc agggtaaata
     1141 tttttaccaa aaactctaaa aatgtcttag gatcgaggat atttcttgta ttatcctgcc
     1201 cctcggcatt acgagcaaaa ttattcattc caacacgcca ggtatttaaa acttcacttt
     1261 tttgtttaaa attgtcggct aatgtaatgt gttgagaata attcaaatct gatattttgc
     1321 tcatgacgga ctccttgagt taataagaac gtctaagcat tacaaatttt actcacaaaa
     1381 aaatatcaaa aaacgctctt ttcacaaact cgccgtaaac ctggtgttat ttacggcgaa
     1441 aagtcatcac gccatcagca tcttcactac tactttgcga acctgggctg gtgcgcccac
     1501 tgcgcacagc ggtttatgca cttcccccgg ataaaacaca acaaaatcac cttcatttag
     1561 gataactgtt ttctcatcaa cgccttccgg caaaaatgcg atgtctttat cagctaacca
     1621 gtcggtatcc ggcgtgcctg caggttgcgt gctgaaggtc atgccttcct gacctcttaa
     1681 cacaatctga atgtcgagat agcgggcatg gtactccgca cggcgagctt cgtacggctc
     1741 ggtcatatct tccgagataa gataaaacag acgattgcct tcgatatcgt gcttaccctt
     1801 tggcgtttct ggcgtaacgt gcgctttgat atgctcaatc gcctggcgta actcctgggg
     1861 tagccacggc tgaagattat gaatatttcc aatgatcatc ttttccactc tttaattaaa
     1921 acaatgtttc atttctatcg ttatacgaaa agatgatcaa ttccgctagc cttaacgcgt
     1981 gaattattgt cttatcacaa cttatcggca ggcgcgttcc cgcctttcgg gtcaaggcca
     2041 gcatcactcc ttgagaatag tttttatcgc ttcccccgcc agtgcagctg ttgcacagag
     2101 ttgttcgatg gagtgcccct cccgtgcctt cgcagaaagc cctgacatca cggtactcat
     2161 aaaatcagtc acacattgtg cgctttgtgg atgccgcctg gcgatatagt cataaatggt
     2221 cgtttctgcg gcgtgataat attgaacggc aatatcacgc gcttgtggat catgactatg
     2281 aataccttca agaaccatac agccagcgca gccgccgttt tggctatatc tgcgcgccgc
     2341 ttcttttaat acctcaacca ggcactcgcc taccggacga tcatcacgaa gaatatcggc
     2401 aagcggaata gcttccgtac cgacatattc attgagtaca cggctaaata acccaacttt
     2461 actgccaaaa gctgcgtaga ggctcggggg gttaatacca agataatcag taacttcagc
     2521 aacactgaca gcatcgaaac ctttttggtg aaaaagcact tttgccgcag agactgcctg
     2581 ctcaggagcg aaacgtctgg gacgacctgg aacgcgagat tgttttttag tgaccattac
     2641 aaaacttgtt gacagaaagt taaaacagtt ttgtaatgca tgttacataa taaatcaagg
     2701 agtccttatg ggcgctttta caggtaagac agttctcatc ctcggtggca gtcgtggaat
     2761 cggtgccgct atcgtacgtc gtttcgtcac tgatggggcc aatgtacgat tcacctatgc
     2821 ggggtcgaaa gatgccgctg aacatctggc acaagagact ggagcgacag cagtatttac
     2881 agatagtgct gacagagacg ctgtcattga tgtcgttcgt aagagcggcg cattggatat
     2941 cctggtggta aatgcaggta ttggcgtctt tggcgatgcc ctggaattaa atgccgacga
     3001 tattgatcgc cttttcaaaa tcaatattca tgctccttat catgcctctg ttgaagccgc
     3061 ccggcagatg cccgaaggcg ggcgcatctt aatcatcggc tccgtgaatg gcgatcgtat
     3121 gcctgttgca ggcatggctg cttatgccgc cagcaaatct gccctgcaag gcatggcgcg
     3181 cgggctggcc cgtgattttg gaccgcgtgg gatcaccatt aacgtcgtcc agccagggcc
     3241 aattgatacc gacgctaatc ccgccaacgg gccaatgcgc gatatgttgc atagtttgat
     3301 ggctatcaaa agacatgggc aaccggaaga ggtcgctggt atggtcgcat ggttagcagg
     3361 gccagaagcc agttttgtta ccggcgcgat gcataccatt gatggcgcgt ttggcgcata
     3421 accgactacg ctcaattaag cccagccatt atccatgatg tctgggtttt gtttactcac
     3481 gtcgtccgct aaaagcggct cccggtaaag agaaatcttc tgttcatgtc gtaaatcttg
     3541 aggataacca aatggtagaa agaaccgctg ttttccctgc tggccgacat tcactgtatg
     3601 ctgagcatcg ttattctgcg gctattcgtt ccggcgattt gctgtttgtt tccgggcaag
     3661 taggaagtcg agaggacgga acaccagaac ccgattttca gcaacaagtc agactggcat
     3721 ttgataattt gcatgcgacc ctggctgctg cgggatgcac ttttgacgat atcattgatg
     3781 ttacgagctt ccataccgat ccagaaaacc aatttgaaga catcatgacg gtgaaaaatg
     3841 aaatatttag cgccccacct tatccaaact ggacggcggt gggtgttaca tggctggcag
     3901 gctttgattt tgaaattaaa gtgatagcgc gcatccctga gcagtaagca agagtgttag
     3961 ccgttcgctt tcacactccg ccctataagt cggatgaatg gaataaaatg catatctgat
     4021 tgcgtgaaag tgaaaaagga aaaagcaggg aatgtctgca attattgata ccgaaggaca
     4081 gttcccctgc agaatcacat caaataaaaa tgcatatacc ttgactttta attcaaataa
     4141 accgtttgcg ctgacaaaat attgcatcaa atgcttgcgc cgcttctgac gatgagtata
     4201 atgccggaca atttgccggg aggatgtatg gttcagtgtg ttcgacattt tgtcttaccg
     4261 cgtctgaaaa aagacgctgg cctgccgttt ttcttcccgt tgatcaccca ttcccagccc
     4321 ctcaatcgag gggctttttt ttgcccaggc gtcaggagat aaaagatggc taatccgcta
     4381 tatcagaaac atatcatttc cataaacgac cttagtcgcg atgaccttaa tctggtgctg
     4441 gcgacagcgg cgaaactgaa agcaaacccg caaccagagc tgttgaagca caaagtcatt
     4501 gccagctgtt tcttcgaagc ctctacccgt acccgcctct ctttcgaaac ttccatgcac
     4561 cgcctgggtg ccagcgtggt gggcttctcc gacagcgcca atacatcact gggtaaaaag
     4621 ggcgaaacgc tggccgatac catttcggtt atcagcactt acgttgatgc gatagtgatg
     4681 cgtcatccgc aggaaggtgc ggcgcgcctg gccaccgaat tttctggcaa tgtaccggta
     4741 ctgaatgccg gtgatggctc caaccaacat ccgacgcaaa ccttgctgga cttattcacc
     4801 attcaggaaa cccaggggcg tctggacaat ctccacgtcg caatggttgg tgacctgaaa
     4861 tatggccgca ccgttcactc cctgactcag gcgttagcga agttcgacgg caaccgtttt
     4921 tacttcatcg cgccggacgc gctggcaatg ccgcaataca tccttgatat gctcgatgaa
     4981 aaagggatcg cctggagtct gcacagctct attgaagaag tgatggcgga agtagacatc
     5041 ctgtacatga cccgcgtgca aaaagagcgt ctggacccgt ccgagtacgc caacgtgaaa
     5101 gcgcagtttg ttcttcgcgc cagcgatctc cacaacgcca aagccaatat gaaagtgctg
     5161 catccgctgc cgcgtgttga tgagattgcg acggatgttg ataaaacgcc acacgcctgg
     5221 tacttccagc aggcaggcaa cgggattttc gctcgccagg cgttactggc actggttctg
     5281 aatcgcgatc tggtactgta aggggaaata gagatgacac acgataataa attgcaggtt
     5341 gaagctatta aacgcggcac ggtaattgac catatccccg cccagatcgg ttttaagctg
     5401 ttgagtctgt tcaagctgac cgaaacggat cagcgcatca ccattggtct gaacctgcct
     5461 tctggcgaga tgggccgcaa agatctgatc aaaatcgaaa ataccttttt gagtgaagat
     5521 caggtagatc aactggcatt gtatgcgccg caagccacgg ttaaccgtat cgacaactat
     5581 gaagtggtgg gtaaatcgcg ccccagtctg ccggagcgca tcgacaatgt gctggtctgc
     5641 ccgaacagca actgtatcag ccatgccgaa ccggtttctt ccagctttgc cgtgcgaaaa
     5701 cgcgccaatg acatcgcgct caaatgcaaa tactgtgaaa aagagttttc ccataatgtg
     5761 gtgctggcca attaattgcg gttggtaata aaagtctggc tccctataat gagccagact
     5821 ttttaccgct gtaataaagg agaaatcatg agcaaaacta tcgcgacgga aaatgcaccg
     5881 gcagctatcg gtccttacgt acagggcgtt gatctgggca atatgatcat cacctccggc
     5941 cagatcccgg taaatccgaa aacgggcgaa gtaccggcag acgtcgctgc acaggcacgt
     6001 cagtcgctgg ataacgtaaa agcgatcgtc gaagccgcag gcctgaaagt gggcgacatc
     6061 gttaaaacca ccgtgtttgt gaaagatctg aacgacttcg caaccgttaa tgctacctac
     6121 gaagccttct tcaccgaaca caatgccacc ttcccggcac gttcttgcgt tgaagttgcc
     6181 cgtctgccga aagacgtaaa aattgagatc gaagcgatcg ctgttcgtcg ctaatcttga
     6241 tggaaatccg ggctatcatg cccggattaa atctgatgac aaacgcaaaa ttgcctgatg
     6301 cgctacgctt atcaggccta cgtgattcct gcaattgatt gaatttgtag gccggataag
     6361 gcatttacgc cgcatccggc atgaacaacg ctcactttgt ctgcaatctg aatcggggct
     6421 atcgtgccca gtttattctt tactgccagc cgtaacgacg gctatagaac cctttcacca
     6481 actgggttaa cgtcatatac cctgccagaa tcgcaaccag ccacgggaaa tagcttaacg
     6541 gcagcgcctg cagctgcaga taactggcca gcggtgaaaa cggcaatgcg atcccgacaa
     6601 tcatcacgat cacggtcatg atcattaacg gccacgatgc acagctctga ataaacggca
     6661 cacggcgggt acggatcata tgcacaatca gcgtttgcga cagtaagccc accacaaacc
     6721 atcccgactg gaacagcgtt tgcgtttccg gcgtgttggc atggaatacc caccacatca
     6781 ggcaaaacgt cagaatatcg aagatcgagc tgatcggtcc gaagaagatc ataaagcgcc
     6841 ccagatccgc cggattccaa cgctgcggct tttgaatttg ctcgtcgtcg acgttatcaa
     6901 acgggatcgc cacctgtgac acatcgtaca gcaggttctg aataagcaag tgtaacggca
     6961 acatcggcag gaagggcaag aaagcactcg ccaccagcac gctgaacaca ttaccgaagt
     7021 tagagctcgc cgtcattttg atgtatttca gcatgttggc gaaagtgcga cgtccctcaa
     7081 taaccccctc ttccagcacc atcaggcttt tttccagcag gatgatatca gccgcttcac
     7141 gggcgatatc taccgcgccg tccacagaaa tgccgatatc cgcagcgcgt aaggcgggcg
     7201 catcattaat accatcgccc ataaagccaa ccacatgccc ttcgcgcttc agtaaggtca
     7261 caatgcgttc tttatgcatc ggcgtcagac gagcaaacag cgtggtgcgc tgtgcgagat
     7321 tagccagttc gtcgtcagat agcgtttcaa tatcactacc aatgaccacc tctcccgcgt
     7381 ccagccccac ttcatggcac actttcgctg caactaactc actgtcgcca gtaaggattt
     7441 ttacggtaat tccactcgct tttaatgcct tcagtgccgg agcagttgtc tcttttggcg
     7501 gatcaaggaa agcaatatat ccttcgagga tcaggtcgga ttcatccgcc cgctggtaat
     7561 ctccttcacg cgctggcagg tatttcgtcg ccaccgcaac cacgcgcagc ccctgacgat
     7621 tcagcgtatc agtaacccgc ttaatcttac gcagcatgat gtcatcgagc ggcacaatct
     7681 cgccattgtg acgcacctgc gaacacacat tgaggatttc ctgcaatgca cctttgcaaa
     7741 ccagctgatg gtgttcggta ttttctgcca ctaccaccga catccgacga cgctcgaaat
     7801 caaacggaat ctcatcaatt ttttgccaac ggctggccag cgagcgcgct gactcttcat
     7861 ccgtaccttc gagcaccgct gtatcgagca ggtttttaag tccggtctga taatgactgt
     7921 tcaaccacgc gctatgcagc acgcgttcgc tggttttacc ggagatatcg gtatgattct
     7981 ccagcacaat tttgtcctgt gtcagggtgc cggttttatc agtgcacaga atatccattg
     8041 cgccaaagtt ctgaatagca tccagatgtt tgacgatcac tttctgtttc gacagcttta
     8101 ctgcgccgcg cgccagcgtc gaggtaacaa tcatcggcaa catttccggc gttaggccta
     8161 ccgcgaccga aagcgcaaac agcgccgctt cccaccagtc gcctttggtg tagccattga
     8221 ttaacagcac caccggtgcc atcaccagca taaagcgaat cagcagcata ctgacgcggc
     8281 tgatcccttg ctgaaaggca ttcggctcgc tttcctgctc actaacacgc cccgccagtt
     8341 gaccaaacca ggtattggca cctgtagcaa tcaccattgc ttgtgccgtg ccgctcacca
     8401 cggtggtgcc cataaaacac agggtgtcgc actccagcgg attgctgtgc tccggctggc
     8461 gagtggttgc ggctttttct acgggcagag actcaccggt taacgacgcc tgagcgacga
     8521 acagatcccg cgcctgcaag atacgtaaat ctgccgggat catatctccc gccgccagtt
     8581 ttataatatc gccgggcacc agctggtcga tcgggatctc cagccagcca ttttcgcctt
     8641 tgtcgttaat tacgcgcagc accgtcgcag tattgctgac catcgctttc agggcatctg
     8701 ccgctttagt ggaacgtgct tcctgaataa agttcagcaa cgtagaaatg gcgaccatta
     8761 gcgcgataac gcccgcggca aataaatctt ccgtggcgta agaaatagcg ccgagaatgg
     8821 tgagtaaaat attaaagggg ttgcgatagc agacccataa atgtacccac cacggcgacg
     8881 gttgttgtgc gggtaattta ttttcaccat gttgttcgcg ggcagattcc acttccgcct
     8941 gatttaaccc ttccggatgg gtgtcgaacg ttttccacaa ttcttcttcg ggcatcaccg
     9001 ccattttcag gcaatgcgca cttaaggacg gcgggaccac cgtattcact gtctgctgcg
     9061 cgccaggcaa tggatcacga tgaaccagac gggaaggtaa atggcgaatg agccgggtaa
     9121 aaatttcttt aaacataagg agtccctccg cactgtctga aacagtacag aaatgtcttc
     9181 aggcgtggcg aagccttacc tgaaggtaat aaagctgagc agggacgtta aaacgtcacg
     9241 gccttacgca tccggtaacg ccgtgaaaag acgggcttac cgaaaaagtt ttaatctccg
     9301 tcgagggaga ggcgtgggat caggttccat ataacctccg gtaagtgaaa aaagcgccgc
     9361 aaataataag gagaatccaa taatattcgt agcatttatg gcgatataat acctgctggc
     9421 aactaaacca acgataaacc agactttacc cattgctgaa tgcacgggta aagttagcct
     9481 caaataatta aacaacacgt tacgggacaa caggatgcaa aatcggctga ccatcaaaga
     9541 catcgcgcgc ttaagcggcg tggggaaatc tacggtttcc cgggtgctga ataacgaaag
     9601 cggcgtgagc cagcgcacgc gcgagcgcgt ggaagcagtg atgaatcagc atggattttc
     9661 cccttcccgc tctgcgcgcg ctatgcgtgg gcaaagcgat aaagtggtcg ccatcattgt
     9721 tacccgcctg gattcgttgt cagaaaatct cgccgttcaa accatgctgc cagcgttcta
     9781 tgaacaaggt tacgacccaa tcatgatgga aagtcagttt tccccgcaat tagttgccga
     9841 acatttgggg atcctaaaac ggcgtaatat cgacggcgta gtgctgttcg gttttaccgg
     9901 cataacagaa gaaatgttag cccactggca gtcatcgctg gttctgctgg cgcgtgacgc
     9961 aaaaggcttt gcttcggtct gttatgacga cgaaggggca atcaagatcc tgatgcaacg
    10021 gctctatgac caggggcatc gtaatatcag ttatctcggc gtgccgcaca gtgacgtgac
    10081 gaccggtaag cgacgtcacg aagcctacct ggcgttctgc aaagcgcata aactgcatcc
    10141 cgttgccgct ctgccagggc ttgctatgaa gcaaggctat gagaacgtag caaaagtgat
    10201 tacgcctgaa actaccgcct tactgtgcgc aaccgacacg ctggcacttg gcgcaagtaa
    10261 atacctgcaa gagcaacgca tcgacacctt gcaactggcg agcgtcggta atacgccgtt
    10321 aatgaaattc cttcatccgg agatcgtcac cgtagatcct ggttacgccg aagctggacg
    10381 ccaggcggcc tgccaattga tcgcacaggt aaccgggcgc agcgaaccgc aacaaatcat
    10441 catccccgcc accctgtcct gatcgtttcc tgaacgataa attgtgatct tcgctgcgtt
    10501 tcgggaacgt tcccgttttt aaatttttcc gcgcaatata ttctgcagcc aaccaaaaat
    10561 gtcatctgcc atggggcttt atgatgagca aaataaacca aacggatatc gatcggttga
    10621 ttgaactggt cggcgggcgc ggcaatattg cgacggtgag ccactgtatt actcgcctgc
    10681 gctttgtcct caaccaaccg gccaatgcca gaccgaaaga aattgagcaa ctccccatgg
    10741 tgaaaggctg tttcaccaat gccgggcaat ttcaggtggt gattggcacc aatgtgggtg
    10801 attactatca agcactgatt gcgtcaaccg gacaggcgca ggttgataaa gagcaggtaa
    10861 aaaaagccgc ccggcagaat atgaaatggc atgagcagtt gatctctcat ttcgcggaga
    10921 tcttcttccc gttgctgccc gcgttgatta gcggcggttt gatcctcggt tttcgcaatg
    10981 tgatcggcga tttgcccatg agcaacggtc aaacgctggc gcaaatgtac ccttccctgc
    11041 aaacgatcta cgattttctg tggttgatcg gtgaagcgat cttcttctac ctgccggtcg
    11101 ggatttgctg gtcagcggtg aaaaaaatgg gcggcacgcc gatccttggt atcgtacttg
    11161 gcgtaacgct ggtttctcca cagctgatga acgcttatct gctcgggcag cagctgccgg
    11221 aagtgtggga ctttggcatg ttcagcatcg ccaaagtagg ctatcaggcg caggtgatcc
    11281 cggcactgtt agccgggctg gcgctgggcg ttattgaaac tcgccttaaa cgcatcgtgc
    11341 cggattacct ctatctggtg gtggtgcccg tctgttcgct gatcctcgcg gtgttcctcg
    11401 cccatgcgct gattggtccg tttggacgca tgattggcga tggcgttgcc tttgcggtac
    11461 gtcacctgat gaccggcagc tttgcaccta ttggcgcagc attgtttggc ttcctgtacg
    11521 ccccgctggt gatcaccggt gtacaccaga ccacgcttgc tattgatttg cagatgattc
    11581 aaagcatggg tggtacgcca gtgtggccgc tgattgcgct gtcgaatatc gctcagggct
    11641 ccgccgtgat aggcattatc atttccagcc gcaagcacaa tgaacgcgag atctccgtgc
    11701 ctgccgctat ctccgcctgg cttggggtca ctgagcctgc aatgtacggc atcaacctga
    11761 aatatcgctt cccgatgctg tgcgcgatga ttggttctgg tctggcagga ttgctatgcg
    11821 gcctgaacgg cgttatggcg aatggcatcg gcgtaggcgg cctgccggga attctctcga
    11881 ttcaaccgag ctactggcag gtgtttgcgc tggcaatggc tatcgccatc atcatcccga
    11941 ttgtactcac ctcgtttatc tatcagcgga aataccgcct gggcacgctg gacattgttt
    12001 aattttcttc ggggcgcaat tgcgccccct cgcattcgca ggaataacgt aatgactaat
    12061 cttccccact ggtggcaaaa cggcgttatc taccagattt atccaaagag ttttcaggac
    12121 accacgggta gcggtaccgg cgatttacgt ggcgttatcc aacgcctgga ctatctgcat
    12181 aaactgggcg ttgatgccat ctggctaacc cccttttatg tctctcccca ggtcgataac
    12241 ggttacgacg tagcgaacta tacggcgatt gatcccacct acggcacgct ggacgatttt
    12301 gacgaactgg tgacgcaggc aaaatcgcgc gggattcgta tcattctcga tatggtgttt
    12361 aaccatacct ctacccaaca tgcctggttt cgcgaggcgc tgaacaaaga aagcccttac
    12421 cgccagtttt atatctggcg cgatggcgaa ccagaaacgc caccgaataa ctggcgttca
    12481 aaatttggcg gtagtgcgtg gcgctggcat gcggaaagcg aacagtacta tttgcatctc
    12541 tttgcaccag aacaggcgga tctcaactgg gagaatccag cggtacgcgc agagctgaaa
    12601 aaagtctgtg aattctgggc cgatcgtggg gtcgatgggt tgcgcctgga tgtggtgaat
    12661 ctgatctcca aagacccgcg tttccctgaa gacctggatg gcgacgggcg tcgcttctac
    12721 accgacgggc cacgagcaca cgagtttttg cacgagatga accgcgatgt gtttacgcca
    12781 cgcgggttaa tgaccgtagg tgaaatgtcc tccaccagcc ttgagcattg ccagcgatac
    12841 gcggcactgg caggcagtga attatcgatg acctttaatt ttcatcacct gaaggtcgat
    12901 tatcccggtg gtgaaaaatg gacgctggct aaacctgact ttgtggcgtt gaaaacattg
    12961 ttccgccact ggcaacaagg aatgcacaac gtagcatgga atgccttgtt ctggtgtaac
    13021 cacgatcagc cgcgcattgt ttctcgcttt ggtgatgaag gtgaataccg cgtgcctgcg
    13081 gcaaaaatgc tagcgatggt gctgcatggc atgcagggaa cgccgtatat ctaccagggc
    13141 gaagagattg gcatgaccaa cccgcatttc acgcgcatta ctgactatcg cgacgtggag
    13201 agcctcaata tgtttgccga gctgcgcaac gatgggcgtg atgccgacga gttattggca
    13261 atcctcgcca gtaaatcccg tgacaacagc cgcacgccca tgcaatggag caacggcgat
    13321 aatgccgggt ttacggctgg cgaaccgtgg attggcctag gcgataacta tcaacaaatc
    13381 aacgtagaag ccgcgctggc cgatgattcc tcggtgtttt acacctacca aaagttaatc
    13441 gcactgcgta agcaggaagc catcctgaca tggggcaatt accaggatct gctgccaaac
    13501 agccctgtat tgtggtgcta tcgccgtgaa tggaaggggc aaaccttgct ggtcattgcc
    13561 aaccttagcc gtgagatcca accctggcag ccagggcaaa tgcgcggcaa ctggcagctt
    13621 gtgatgcata actacgaaga agcctcacca caaccctgtg ccatgaattt acggcctttt
    13681 gaggctgtct ggtggttaca gaagtaaatc ttccctaagc cccggtaatg ccggtcattc
    13741 cggggttttg tctgtcagca tgttgttttt gttgattttt caaccagcaa attcattaaa
    13801 aaatttacat aacgctgtag cgcccgtcat ccgtacgctc tgctttttac tttgagctac
    13861 atcaaaaaaa gttcaaacat ccttgatgca aagcactata tatagacttt aaaatgcgtc
    13921 ccaacccaat atgttgtatt aatcgactat aattgctact acagctcccc acgaaaaagg
    13981 tgcggcgttg tggataagcg gatggcgatt gcggaaagca ccggaaaacg aaacgaaaaa
    14041 accggaaaac gcctttccca atttctgtgg ataacctgtt cttaaaaata tggagcgatc
    14101 atgacaccgc atgtgatgaa acgagacggc tgcaaagtgc cgtttaaatc agagcgcatc
    14161 aaagaagcga ttctgcgtgc agctaaagca gcggaagtcg atgatgccga ttattgcgcc
    14221 actgttgccg cggttgtcag cgagcagatg cagggccgca accaggtgga tatcaatgag
    14281 atccagaccg cagttgaaaa ccagctgatg tcgggtccat acaaacaact ggctcgtgct
    14341 tacatcgagt accgtcacga tcgcgacatt gaacgtgaaa aacgcggtcg cctgaaccag
    14401 gagatccgtg gtctggtcga gcagaccaac gcctcgttac tcaacgaaaa cgccaacaaa
    14461 gacagcaagg tgattccaac ccagcgcgac ctgctggccg ggatcgtggc taaacactat
    14521 gcacgtcagc acctgctgcc gcgtgacgtg gtgcaggcac atgagcgtgg cgatattcac
    14581 tatcacgatc tcgattactc accgtttttc ccgatgttca actgcatgtt gatcgacctg
    14641 aaaggcatgc tgacccaggg ctttaaaatg gggaacgccg agattgaacc gccgaagtcg
    14701 atctcaacgg caaccgcggt aactgcgcag attattgctc aggttgccag ccatatttat
    14761 ggcggcacca ccattaaccg tatcgatgaa gtgctggcac cgtttgtcac cgccagctac
    14821 aacaaacatc gcaagaccgc agaagagtgg agcatcccgg acgcagaagg ctacgctaac
    14881 tctcgtacca ttaaagagtg ctacgatgcc ttccagtcgc tggagtacga agtaaacacc
    14941 ctgcataccg ccaacggtca aacgccgttt gtaacttttg gttttggtct gggcaccagc
    15001 tgggaatcgc gcctgattca ggaatctatc ctgcgtaacc gtatcgcagg tctcggtaaa
    15061 aaccgtaaaa ctgcggtgtt cccgaaactg gtgtttgcga ttcgcgatgg tctgaaccat
    15121 aaaaaaggcg atccgaacta cgacatcaaa cagctggcgc tggagtgcgc aagcaagcgc
    15181 atgtatccgg atatcctgaa ctacgatcag gtagtgaaag tcaccggttc gtttaaaacc
    15241 ccgatgggct gccgcagctt cctcggcgtg tgggaaaatg aaaacggcga gcagatccac
    15301 gatggtcgta acaacctcgg cgtgatcagc ctgaacctgc cgcgtattgc tctggaagca
    15361 aaaggcgatg aagccacctt ctggaagctg ctggatgaac gtctggtgct ggcacgtaag
    15421 gcgctgatga cccgtatcgc tcgtctcgaa ggcgtgaaag cgcgcgtggc cccgatcctc
    15481 tatatggaag gtgcttgtgg cgtgcgtctc aatgctgacg atgatgtttc tgaaatcttc
    15541 aaaaacggtc gtgcgtctat ttcgctgggt tacatcggca tccacgaaac cattaacgcg
    15601 ctgttcggcg gcgagcatgt ttacgacaac gagcagcttc gcgcgaaagg tatcgccatt
    15661 gttgaacgtc tgcgtcaggc agtggatcag tggaaagaag aaacgggtta tggtttcagt
    15721 ctctacagca cgccgagtga aaacctgtgc gaccgcttct gccgtctcga tactgctgag
    15781 tttggcgtgg tgccgggcgt gaccgataaa ggttactaca ccaacagttt ccacctcgat
    15841 gtggagaaga aggtgaaccc gtacgacaag atcgactttg aagcgcctta cccgccgctg
    15901 gcgaacggtg gtttcatttg ctacggcgag tatccaaaca ttcagcacaa cctgaaggcg
    15961 ctggaagatg tctgggatta cagctatcag catgtaccgt attacggcac caatacaccg
    16021 attgatgagt gctacgagtg tggctttacc ggtgagttcg agtgcaccag caaaggcttc
    16081 acttgcccga aatgtggtaa ccatgacgcc tcccgtgtgt cggtaactcg ccgcgtgtgc
    16141 ggatatttag gtagcccgga tgcacgtcca tttaacgccg gtaagcagga agaagttaag
    16201 cgccgcgtta aacatttggg aaatgggcag ataggttaat cttcactcta cgttaagcaa
    16261 atgccggatg cggcgtaaac gccttatccg gcctacattt ggcaactgct ccaggcctga
    16321 taagacgcga cagcgtcgca tcaggcattg attaccggat gcagcctaca cgcggactga
    16381 taagccaggc gaaataatga attatcatca gtactatcct gtcgacatcg tcaacggccc
    16441 cggcactcgc tgcaccctgt ttgtctccgg gtgtgttcat gaatgccccg gttgctataa
    16501 caaaagcaca tggcgggtaa attctggtca gccatttacc aaagcaatgg aagaccagat
    16561 cattaacgat ctgaatgaca ctcgtatcaa acgccagggg atttccctct ccggcggcga
    16621 tccgctgcat ccgcaaaacg tgccggatat tctgaaactg gtaaaacgca tccgcgccga
    16681 gtgtccggat aaagacatct gggtgtggac aggctataaa ctcgacgatc tcaacgctgc
    16741 gcaaatgcag gttgttgatt tgattaacgt actggtcgac ggcaaatttg tgcaggattt
    16801 aaaagaccct tccctgatct ggcgcggcag cagcaatcag gtggtgcatc atttgcgatg
    16861 ataagttccc tgttacctga gtgcaggcaa cagggaaatg aggaattaac gatacttctg
    16921 gtgataggcg ttgcgggtcg ttttcagttg ctctgcggca gcctgcgctt cttttacttt
    16981 accttcattt gccagcttca gcgcgtcgtc aatctgacca accagaatat cgaaaccgtg
    17041 acggaagtct ttcatttccg ggctgtccgg tgatttatct tcgagcttcg gcggcgttgc
    17101 tttttgcgca tccagcgctg cggcgcgcat cttcgttaac gagtctttga cttgcgccgc
    17161 gttatcggct ttttcgacca cttttaaagt gtcgttgagg gtttccatat tgtcttcaag
    17221 atcagcggca aacgacgccg aactgaatac caacgaggag actgcaagaa tagctaacag
    17281 gcttttacgc attgctcact tcctttttta tttacacttc atccttcaca ctgcctcatc
    17341 gacggctacg ctcattcgcc cgggtcacgt actgcgagta cgctccccgg ccctcattca
    17401 ctttccgtct cgatgcagtg cgaatgattt tgtgtaattt tttagtttat agcgcggcag
    17461 gtcgcgccag ttttttactg cccggcgatt ttcatctccg gcaacagcac agaaccacac
    17521 tgtatattac tgcgtgtttc aatatcgtta ccgacggtga caatattgcg ccacatatct
    17581 tttaaattac ctgcgatggt gatttcgcta accggatact gaatttcgcc attctctacc
    17641 cagaagcccg ctgcaccacg ggaataatca ccggtaatgg cactcacgcc ctggcccatc
    17701 agctccgtca ccaccagccc ggtacccatc tctttgagca tttgttcgaa gcttagccct
    17761 tgtccggcaa tccgccagtt gtgaataccg cccgcatgtc cggtgctttt cagccccagt
    17821 ttccgcgccg agtagctggt cagcagccac tgagtcagga tgccatcttt aataatatcg
    17881 cgacgctcgg tgcgcacacc ttcgctgtca aatggcgtcg atgccagccc tttcagcaga
    17941 tgcggatgct cttcaatggt cagccagtcc ggcagaattt gtttacccag cgaatccagc
    18001 aggaaggtgg atttacggta aaccgatcca cccgctatcg cccccaccag atggccaaaa
    18061 agcccggttg ccacttcatt ggcaaaaatg accggcgctt tcatggtgga gagtttacgc
    18121 ggtgacaggc gcgataaagt acggcgagca cagtcggccc caacccactc tggcgtttgc
    18181 agatcgctca tcgcacgacc aatggtgtag gcgtaatcac gctccatatc gccattttct
    18241 tcggcaatta cacagctgga gagcgaatga cgcgttgagc agtaaccctg caacatgccg
    18301 tggctgttgc caaaaacttt gacaccgtag tggctgttaa agctgccacc ttcggtattg
    18361 gtgatgcgtt tgtccgcctg caatgccgcc tgttctgcgc gggccgccag ttcaatggct
    18421 tcatccgggg aaacttccgc agggtggaac aagtcgagat ctggtgcgtc aaaggccagc
    18481 agctctttgt ctgccacgcc ggcacaggga tccggcgagg tataacgggc aatatccagc
    18541 gccgcctgta cagtacgggc aatggcctgc gggcttaaat cggtggatga tgcgctacct
    18601 ttgcggttct ggtgataaac agtgatcccc agcgcgccat cgctattgaa ttcaacattc
    18661 tccacttcac cataacgcgt gcttacgcta atgccggtgg tcttgctgac agcaacttcc
    18721 gcaccgtccg atttgcctga ggccaactcc agcgcagtgg aaactgcttc ttccagaatc
    18781 ttgcgctgcg cttcaacttg agagattact ttcattgcaa gtgccataat gtagagagtt
    18841 tctctgaagt ctaacagaga accgtttttc agtgcgcatc ttaactggta acattagcct
    18901 ctttttttta aggagcctga gatgactaag cagcccgaag actggctcga cgacgttccc
    18961 ggtgatgaca tcgaagacga agacgatgaa attatctggg tcagtaaaag tgaaattaaa
    19021 cgtgatgccg aggagctaaa acgccttggc gcggaaattg ttgatctggg gaaaaacgcg
    19081 ctggataaga tcccgttaga tgcggatctg cgcgcggcta ttgagctcgc ccagcgtatt
    19141 aagatggaag gtcgccgccg ccagttgcag ctcatcggta agatgctgcg ccagcgcgac
    19201 gtagagccta ttcgtcaggc gctggataag ctgaaaaacc gtcacaacca gcaggtggtg
    19261 ctgttccata aactggaaaa cctgcgcgat cgtctgatcg atcaaggtga tgacgccatc
    19321 gctgaggtat taaatctgtg gcccgatgcc gatcgtcagc aactgcgtac tctgatccgc
    19381 aacgcgaaga aagagaaaga agggaataag ccgccgaaat ccgcacgcca gattttccag
    19441 tatctgcgcg aactggcgga gaatgaaggg taattttcgt tgttgatgtg agtatggttt
    19501 gccggatgcg gtgcacctgt aggcctgata agacgcatca agcgtcgcat caggcactat
    19561 gctcaaatgc cggatgcggc gtgaacgcct tatccggcct acgcacacta tatcaggcta
    19621 aggccgaatt actgcgcggc ttccgccttc ttcgccagac catccagcag tttctgatgg
    19681 atcccaccaa aaccgccgtt gctcatcacc agaatatggt cgccaggctg agcggttttc
    19741 accaccatat ctgccagcgt atccacatcg ccactccagt gtgcaggctg aacgcaggct
    19801 tctgccactt ctgccacctg ccacggaata tgcgccggtt gcagcaggaa gacttcatcg
    19861 gcacgaccta atgaaggtgc cagatcgtct ttgcagatcc ccattttcat ggtattcgag
    19921 cgcggttcca gcacggcaat aatgcgcgcc gtaccgccaa ctttgccacg cagcgccgca
    19981 agcgttgcca gaatcgccgt tgggtgatgg gcaaaatcgt catataccgt gacgccattc
    20041 gcttcaccac gcaactccag acggcgacga gcgttaataa acgaacccag cgcgttagcg
    20101 gcatctgccg gcgctacacc aacatggcga gccgccgcaa tcgccatcag gccattgtgc
    20161 atattatgtt cgcctaccag cgaccatttc acttcgccca ctttttcgcc atccagcaaa
    20221 acttcccatt cggaagcatc ggtggtcagc tttttcgcct gccagtgccc ctgctcaccc
    20281 accagctcct gctcgctcca gcagcccatc gccatggtct gtttcaggtt gatgtcgttt
    20341 tctggccaga taatacggcc ctgccccgga acgatacgca ccagatggtg gaactgtttc
    20401 tggatcgctt tcaggtcgtc aaagatatcg gcgtgatcga actcaaggtt gttgaggatc
    20461 agcgtacgtg ggcagtaatg gacaaattta gagcgtttgt cgaagaaggc gcagtcatac
    20521 tcatccgctt cgataacaaa gaagtcgctt tcgcccagac gcgccgaaac ctcaaagttc
    20581 cccggcacac cgccgatcac aaatcccggt ttgtaaccgc actgttccag aatccaggtt
    20641 gccattcccg cggtggtggt tttgccatgt gtaccggcaa cggccagcac ccagcggtcg
    20701 cgcagcacaa aatcgtgcag ccactgtgga cctgacatat aagggatgtt tttttccagt
    20761 accgcttcca cacacggatt tccacgggtc atggcgttgc caataatcac cagatccggc
    20821 tgcggatcga gctggctggc atcgtaaccc tgaatcagtt caatgccttg cttctcaagt
    20881 aaggtgctca tcggcggata cacattggcg tccgaacccg ttacttcatg gcctaactgg
    20941 cgcgccagca tcgccagacc gcccataaac gtgccacaaa ttcctaaaat atgaatgcgc
    21001 atacgtcact atcctttttt aatctgccgc tcattttacg catatgtcgg gcaagtgaga
    21061 aacgcatttc aggataatcc gcaatttgct ggcgcgattc acctgagcgc aacattgaga
    21121 ttatttgtta agattgttgc ggtcgcttta ctccataaac attgcaggga aagttttatg
    21181 aaaacgttag gtgaatttat tgtcgaaaag cagcacgagt tttctcatgc taccggtgag
    21241 ctcactgctt tgctgtcggc aataaaactg ggcgccaaga ttatccatcg cgatatcaac
    21301 aaagcaggac tggttgatat cctgggtgcc agcggtgctg agaacgtgca gggcgaggtt
    21361 cagcagaaac tcgacttgtt cgctaatgaa aaactgaaag ccgcactgaa agcacgcgat
    21421 atcgttgcgg gcattgcctc tgaagaagaa gatgagattg tcgtctttga aggctgtgaa
    21481 cacgcaaaat acgtggtgct gatggaccca ctggatggct cgtccaacat cgatgttaac
    21541 gtctctgtcg gtaccatttt ctccatctac cgccgcgtta cgcctgttgg cacgccggta
    21601 acggaagaag atttcctcca gcctggtaac aaacaggttg cggcaggtta cgtggtatac
    21661 ggctcctcta ccatgctggt ttacaccacc ggatgcggtg ttcacgcctt tacttacgat
    21721 ccttcgctgg gcgttttctg tctgtgccag gaacggatgc gcttcccgga gaaaggcaaa
    21781 acctactcca tcaacgaagg aaactacatt aagtttccga acggggtgaa gaagtacatt
    21841 aaattctgcc aggaagaaga taagtccacc aaccgccctt atacctcacg ttatatcggt
    21901 tcactggtcg cggatttcca ccgtaacctg ctgaaaggcg gtatttatct ctacccaagc
    21961 accgccagcc acccggacgg caaactgcgt ttgctgtatg agtgcaaccc gatggcattc
    22021 ctggcggaac aagcgggcgg taaagcgagc gatggcaaag agcgtattct ggatatcatc
    22081 ccggaaactc tgcaccagcg ccgttcgttc tttgtcggca acgaccatat ggttgaagat
    22141 gtcgaacgct ttatccgtga gttcccggac gcgtaatctc ctgcgcctct ccacacggag
    22201 aggcgttttt atcgctgcgc aatgttgact cttgtcacag gcgaactctg acgattctcc
    22261 cacagcaccg ttaatccacg ctgtaatgct ataaaaataa acaacaaaat gccgatggcg
    22321 attttcgtcc accaggaact cagcgtaccg tcaaagttga tatatgtctg aatcagtccc
    22381 tgaatcgcca cgccaaaaag cgtcccaagt actgttccaa cgccaccgct caaaagtgta
    22441 ccgccaatca ccactgaggc gatagcgtcc agttccacac ctacgcccgc cagcgcatat
    22501 ccggcctggg tataaatcga gaagacaatc cccgccagcg ttgccagtcc ggtggagagc
    22561 atataaatgc gaatagtggt gctgcgagtg gaaatcccca tcaggttcgc cgacgttgcg
    22621 ttgccgccaa tggcgtatac ctgattacca aaacgggtac gatgcgcgag gaatatgccg
    22681 ataaccacca ccgccagcat cagcagcccc atcgcactta agcgaccgcc gccagggatt
    22741 ttccatgcaa ggcttgagag cgtgtcataa acaggatgat ttatcgggat cgactcttcc
    22801 gaaacgagat agctgacgcc acgcaaaaag aacattcctg ccaaagtaat gataaacgcc
    22861 gggatcttca gggcgtcgat caaaagcccc ataaatgcac caaaggcaca gcccatcacc
    22921 agcaccagtg gaaacgccag cagcggcgag aggccgaaat cgccaatcac ttttgccaga
    22981 aacacgccgg taaaggcgat caccgaaccg acggagagat cgatcccacc ggagaggatc
    23041 acaaaggtca tgccaacggc gatgatcccc agaaaagcat tatcggtcag gatattgcag
    23101 atcactcttg tggaagcaaa accgggaaac tgcgtcagac agtagagata acccagcaca
    23161 aagacgccga tggtgatcat cagcggcagg ttacgtttta tcacggctac gcactccttt
    23221 aatcagactg ataaagcgtt gcgactgaac aatcagcacg caaagcacca ctaccgcttt
    23281 caccacctgg ttcatctctg gcggaaagcc agaaagtaaa attccggtgt tcatcccctg
    23341 aataatcagc gcccctacca ctgagagcag caggttaaaa cgcccgccca tcagcgatcc
    23401 gccgccaatc accaccgcaa gaatggcgtc cagctccagc cataatccgg cgttgttggc
    23461 atcggcaccg cgaatatccg ccgccacgat aatgcccgca atcgccgcac atagcccgct
    23521 caacacgtag gtgagcatga cgatgattcg cgtgtttacc ccggcatttt ttgccgcccg
    23581 aatattgata ccaacggctt cgataaacat ccccagtgcc gttttgcggg tcaacagcca
    23641 gaacaggaga agcgtcagca ccgcaataat gactggcgtt ggcaggaaca acagcgatcc
    23701 actgccgaac caggagagat ccggtgagtt aaatgtgacg atctgtccgg aggtgatcag
    23761 ttgcgccacg ccgcgcccgg cgaccatcag gatcagggtg gcgacaaacg gctgaatttt
    23821 gaggatcgct accagtatgc cgttccacaa tcctgccagg atgccagtgc ccagggcgct
    23881 taacaaaaca attggcaggc tgaatcccgc gaccgtcatc gcagccattg tggctccggc
    23941 gatagccatc accgccccta cggagagatc aatcccacca gtggcgataa ccagcgtcat
    24001 gccaatcgcc agtagcgcaa cgggggccgc acggttaaga atgtcgatag ggctaccgaa
    24061 caaacgccca tcctgaagca ccacctgcca gaaatgcggg gccaccaggc tatcgaccag
    24121 caacaccagc aatagcgccg ccagctgcgg cattcccgtt ggccagcgaa agcgcctttt
    24181 cggcggcgta gtgtccggga gagattgagg catcacactg ttctccttac gccgcaatgg
    24241 cgttcatgat cgccggaacg gaaagctctg ccagcgggat ctccgccacc tgtttgcgat
    24301 cgcgcatgat aatcacccgg tcggcatagc ccaccagctc ttccagttcg gaggagatca
    24361 ccagcagcgc cagaccatca gcgcacagcg tttcaatcag gcggatgatc tcggcgtggg
    24421 caccaacatc aattccgcgc gttggctcgt cgaggatcag aaattgcggt cgggtcagta
    24481 gccaacgtga aagcaacact ttttgctgat tgccgccaga gagaaattca atcggttgtt
    24541 cagttgaagg tgtgcgaatg ccaagctggc ggataaagcg ttcggcaatc tcttgctgct
    24601 ctttgcggga aatgggtcgt agccagccgc gctgggcctg gagagcgagg atgatatttt
    24661 cccgcaccga ggcggcagcg atgatgccat cggttttcct gtcttccggg cagaagccga
    24721 tgcccagtac cgaagcctga tgtggcgatc gcagggtttg cggtttgcct ttgatcaacg
    24781 ccgtgccgct gtcagcaggt ttgataccga agatcacttc ggcggtttcg gtacgtccgg
    24841 atcccagcaa gcctgccagg ccgacgatct cgccggggcg cacttcgaga tcaaacggtg
    24901 ctatcgttcc ttttttgccg taatttttga acgcggcaac gggtttgtca ctcagcaatg
    24961 ttcgcccggc acgctgtagc gcgtgggtgt ccagctcgcg ccccagcatc atttttacca
    25021 gttcgatctg cggtagctcg cgggtttccc gacagcctac gaaactgccg ttgcgtaaga
    25081 cggtgatccg atcgctaacc tgatagacct gatcgagaaa gtgagtgacg aagatcaggc
    25141 tgacgccgcg atcgcgcaac tgacgcatca ggccaaacag taactcaact tcctgagtgt
    25201 cgagactggc agtgggttca tcgaggatta gcactttggc agagagatcg atagcccggc
    25261 aaatggcgac gatttgctgc atcgcgactg aaaagcggtt gagcggttcg cgcacgtcga
    25321 gggagaaacc gtaagatgcc atcagttcgg tggcgcgctt ttccatctct ttacggcgta
    25381 gaaggccgaa acgtttgggt tcgcgaccta tgaatagatt atcagcgacc gacatattgg
    25441 gtagcaggtt gacttcctga tagacggtgc cgatgccgag ttgttgtgcg tgggcggtat
    25501 ttttcggtga gatagtctgg ccttccagcc agatggtgcc acgatcggcg tggtagacac
    25561 cggttaatgc tttgattagc gttgattttc ccgccccgtt ttcaccgagc agcgccatga
    25621 tttcaccacg gcgcaggttg aaatcaacgt tgtctaacgc tttgacgccg gggaaaaatt
    25681 tacttaatcc ttcggtgcgg aggatttcct ggtgttggtc ggtcgtcatg gtttcccccg
    25741 gtgtaaagcg tgctcccagt aggcatgata agacgcggta agcgtcgcat caggcttcag
    25801 tgcaccatcg ccggatgcgg cgcgagcgcc ttatccggcc tacgaatcgg catcaggcat
    25861 agcaaccctc aataccccat atttttcttc ttctctaact cttcttttgc ggtatcaggc
    25921 agataaaggg tggatttggt cagggtcagc ttttcaggca tggtgccgtc ttttttatat
    25981 ttctccagcg catcaaaagc ggggcctgcc atgttcggcg tcagttcaac gctggcgttc
    26041 gcttcgccat caatcatcgc tttgtagatg tccggcacgc cgtcgataga gcctgtcagg
    26101 atatctttgc ccggtttcag gcccgcttct ttaattgcct gaattgcacc aatcaccatg
    26161 tcatcgttat gggcgtaaac catgcagatg tttttgccgt tgttttccgc tttgataaag
    26221 ctctccatga cttctttgcc tttactgcgg gtgaagtcac ctgactgcga gcggataatt
    26281 ttgatatttg gcgcattctt aatggcttcg gcaaagcctt tcttacggtc aatggcgacg
    26341 ctggccccaa cggtgccctg cagctccacc acgttgcatg gtttgccatt cacttctttt
    26401 accagccagt caccgatcaa tttgccttcg aggatgttgt cggcagtgac ggtggtcata
    26461 tagagagatt tgtctttcac atcgatggaa cgatcgagca aaaagaccgg gatttcggca
    26521 tctttcgcct cttttaatac cggctcccaa ccggtcgcta ccaccggagc gataaagatc
    26581 gcatccaccc cttgcgcgac gaaggaacgt accgctttaa tctggttttc ctgcttttgc
    26641 tgaccatcgg caattttcaa cgtaattccg cgcttttcgg cttcactttt cgccacattg
    26701 gtttctgcgg cgcgccagcc ggattccgat ccgacctgcg aaaatccaac ggttaatgga
    26761 gcggccaacg ccatagacga catggctgcc gagactgcag agactacaag taagcgtttc
    26821 cacatagggt tgtcctcgtc ggggtaatta ttattggtga aaagatgttc gcgaaaaaac
    26881 tatagacaat tcgttatgta acggattgcg ttacatcaca cttcaaaaaa gtaaataaaa
    26941 cgttaatcac aagtttgtaa tcgctttcat ctcactatga aaaatgcggc tacggttatg
    27001 gattttcctg ctctttaaac catcttaaaa ctggctaaaa aggaaaatga agaagaaaac
    27061 aagcgaagac attcggcgcg agttggctat aatactcggc acttgtttgc cacatatttt
    27121 taaaggaaac agacatgagc ttactcaacg tccctgcggg taaagatctg ccggaagaca
    27181 tctacgttgt tattgagatc ccggctaacg cagatccgat caaatacgaa atcgacaaag
    27241 agagcggcgc actgttcgtt gaccgcttca tgtccaccgc gatgttctat ccgtgcaact
    27301 acggttacat caaccacacc ctgtctctgg acggtgaccc ggttgacgta ctggtcccga
    27361 ctccgtatcc gctgcagccg ggttctgtga tccgttgccg tccggttggc gttctgaaaa
    27421 tgaccgacga agccggtgaa gatgcgaaac tggttgcggt tccgcacagc aagctgagca
    27481 aagaatacga tcacattaaa gacgttaacg atctgcctga actgctgaaa gcacagatcg
    27541 ctcacttctt cgagcactac aaagaccttg aaaaaggcaa gtgggtaaaa gttgaaggct
    27601 gggaaaacgc agaagccgct aaagctgaaa tcgttgcctc cttcgagcgc gcaaagaata
    27661 aataagttct tctagcgtaa taaccctgaa cgccgggctt cggttagtag gggtttttta
    27721 tgcccgcgat aaataaacac gccttattcc accaccgcct gcaaacgtaa taacgcctct
    27781 tccaccacct cacccgcagc cagaccaata cgctttgcca gccgggcgcg tagatccatc
    27841 atccgcacct gattcaccag caccacgccg tggacatcgc cttcttcgca atgtaaagga
    27901 acgctgaatc ctgcataacg ggcaaaattt ccgccctgcg taatgggggc caccagcgtc
    27961 attcccagtt gattaaaggc ttgaacggag agcacaagcg caggtcgacc agcaccttgc
    28021 tgttcatggc cgcttgctgg atcaaagcca accagcacaa tgtctccccg ttcaaattca
    28081 cttttcttta ccatatttcg tcacccgcag gggtggattt accccagaca tcctgctcgc
    28141 taagttccgc ggcgttcatg tcgcactgtg ccagcagttc atcaagcgag tagcgcctgg
    28201 agatgggtgt cagaatcagt tgattgttgc tcacttgcgc ctccacgctc tgccccggcc
    28261 gtaagttaag ttctttcatc acaacattag gaatgaccat acctgaactg ttcccccatc
    28321 tttttatggt aatacgcatc tgcacctcct taagttatac aaagtataac tcacggtgct
    28381 acaggaaacg ttttacccct gcacatttgt gaacgccagc agagtttttt atgcgttaac
    28441 agatatggaa agcggctcgg agagaaaagc aaaaggtgag ggaattacaa aacagaatgc
    28501 gagtcgtctc gcaaaaaaca acgccacccg aaggtggcgt atgcgtatgg ttacttatcc
    28561 ctgtctaacc agtcgccgct ttcaattagc tttaatccat cgacgggtcg ttgataaacg
    28621 tacatccatg cactcccgta cggcgtctga atcaactggc gcgcgtattc accgcccctg
    28681 gtgcgcaagg catcaagttc ggccagcgtg gcgttgtcaa tacgataaac ttcaccgtgt
    28741 actgttccgt tccccggaac tgcgcctgga tagtggccca ggctatacaa ctggtagtta
    28801 tcgatactga aatcgcccag taactgggca ttggtcatcc agtgactgtt gccttgtttg
    28861 tggcgtaaac tgccgtagac aaatattcgc attgctaaaa ctcgaactga tagagcaaat
    28921 ccagtgcctg gtctacacca gacacggctt ccagatatag cttaggcatc aggcgataac
    28981 gtaacgtgag tgttgctata gagtcaaata tacccacgcc gtatttcact tgcagacctg
    29041 gcaatacata gccgctgacc accacctggg aggagtcgcc tactccctgg gtgtcgagcg
    29101 ctaaattgct tacgccaaac gtctcgccga ttttacccac aatctgacca ctttgtgcaa
    29161 cccccagacc aatcagcatt gaggtcatcg ccgcactgtc gctctgatcg ctctccagcc
    29221 cttgtccacg tagcaaataa gacaaggccg cctgttgcga catggccggg tcggagaaga
    29281 tctccgcttt cggttcgtcc gccagaccag tgacgcgaac tccggcgatt acgtcgtctt
    29341 ctgtagcatc cgggttacga atagcttcaa tattaagata cggttgatct ggcggaccag
    29401 agaacagtag ctcaccctta cgcacaatca gatcctgacc ataggcatgg aagcgccctt
    29461 cagggatgtt gatctgcccg ttcaggccca gcccttgttt gtcctgaacg acgttaagat
    29521 cgcccgtcag ccgcgctttc aggccaaagg cgtcaatgcg cacattgttg ccaacgtgga
    29581 caatcaggtt actgttaatc ggaatcgacg ccgttttcgg ctcttccggt tgcaggttat
    29641 cgttaagcat caccacatcg ctggagacgc ctactgcgct ttccggcaga tcgtgcacca
    29701 cgatgcgcgc ccacgggaca tccacgcgac catcgagggt aaacaggttt ggtgtagcct
    29761 cgaatacaac atctggcgat acatccattc gtaccatcgg cggcacggtg atccgcactt
    29821 tactgccttt cgccgttact cgtgcccgcc agttttcaat ttggctccag tcggcatcac
    29881 cgttcaggta gatttcaccc tgctgggtcc gtactgtacc ggcaagcgtc gagcgcatac
    29941 cgttaaagtt gaccgcaagc tggctcggct gcatatcaaa cggcataaag ttgccgtcga
    30001 tatccacacc cgtaacctga agctgaccaa acaactgcgg gctttgcaca tcaccaccca
    30061 gacgcaagtt ggcactcacc atccccgctg ctttttcccc acgggtaaag atggggttta
    30121 tcatcgcaag gttgaagtta cggatattga cgttgccacc aagattacgg cggccttgcg
    30181 gatcggtcac ctgcacctgt ccatcaaact ggccgttatt ggtcaggcgg atggtccagc
    30241 ccaattcggc acggttgtta cgcaattccg ccgtcagatt cagtgtctga aacgccaccg
    30301 gcagcgccgc atcgttgacg gtttgcgtta cctgcacgtt acgccccgaa agggtgatac
    30361 tgccctgcgg cagcccctct ttcgtggtgt cccaggcaac atccgcttta cccgtgaaga
    30421 taccgctggc ctgagtggtt tctggcataa acggtttcag catggcgagg tcgaagcggt
    30481 tgagattcac caccgcacgc ccttcggccc ccgcatcgat agtttgcggc acgcacagtt
    30541 ccgcattcgg gttaagccag cagtgtggcc cgatgctgat tttttgctcc ttattgcggt
    30601 aatccagcgc aatatcgcgg gtcagcgacc acgggccgac cggcgtctgg aagcgggtat
    30661 tgctaagagt tcccttccag cgttcttctt tgcgatcaaa acttcctgcc agattaagct
    30721 gccaggagac aggctcgccc tgaatccgca actgtagctc gtgctgcttt tcgctgcctt
    30781 tggcattcag ggtgacgagg ttgatattta catccggctg cgaaatttgc tcaacgcgta
    30841 cgtcgagttt cccggcgatc tgatcggtgg atttaatgtc gccttccacg cgaacctgcg
    30901 ccacggaaag ttcctgccag cgcaggccgc gcgcggtgat atctgccagt agttgtggcg
    30961 cttccaccgt gccgcgtact ttcaccagcc ctttcgctgt accgccaagc cccggcagcg
    31021 cgttatccag ccccggcgcg ttgatggtgg catcaagatt gagatctttt acccccagct
    31081 cgcctttcac ttcggcactg tttggcccga gttccagatg aagcccaggg atcatccact
    31141 gcatataact gttgcctttc agcgtgccgt caacgttcac tttgttctgt ttgacgttac
    31201 cggtcagctt cagttctggc acctccatct gccaggtgcc gccgtacagg ctaccgcggg
    31261 ttttaatcaa gccattgagt ttcgacggcc actccgggaa ctctttggcg gtgttaatgc
    31321 cattaagcgt tagctcaccg cgccaactaa tggcctgctg ccagtcgagc aacgccttga
    31381 gttcagtttt cccttccagc gccgcgacgg tgagtttgtc gagattgacc tgctgttcat
    31441 tacctttggc atcaagggta atggtggctg gcgggatctc ctgtcccttc actgccgtac
    31501 gcatagagag cgtgtaatcg gtcattttgc cggtaagttt cagtttcaga tcatccgcct
    31561 gatactgctt ctcaccagtg aacggccagt aaagctgttt gctgttcact tccacgttga
    31621 gcggcaatcc ggcttccgcc agtcgcgtct gggcgcgtaa atccatatcc accggaccgg
    31681 aaagattaac gccaatctcc agctgttcgc gcagcgcgcc gcccactttt agcttcactt
    31741 tttcaccttt caacggctcc acgttcagtg tgctgttgag ggtgatatcc accggccagt
    31801 tgtctgacag ctgcgccgtg ccgctggcgt tgacgatccc ctggttggaa tcgatatcca
    31861 gggcgtccag tttagtattg ccgtcaatgc tgctcacttt cagcagcatg gtgcgcacgg
    31921 tgatgtccgt gtcgcccgtc acgcgtaact gctcgccttt aaactcttca atgttcaggt
    31981 taagcggcaa atgcaagtcg gtcatttccg gcaataccgg gcgagaaaag agatctttca
    32041 gcgtttcgcc gagcggcttt tcctccggct gcggattttc aattttcggt tcgaccactt
    32101 cttcctgcgc cacttccgcc actttcggca gagcaatcag caggcctttc agcgacgtcg
    32161 gtttcagggt cagggttttc tcctgccagt tcaggccgga ggtgaagtcc atcaccgaca
    32221 ccgtggtgtc atcaatcttg atgttgacgt tgtccagcgc cacccgtgtc agggtgatgg
    32281 gatacggcgt ggagagatcc agcggaccgc tatcttcctc ttcttcaacc tgttcagaag
    32341 gaggcatttt tttactgtcg atgttgacct gaatgtcttt cagcgccagg tcattaatac
    32401 aaacgctact gttccacagg cactcaagac cgaccgccag atgcagatta cccgctttta
    32461 ccgccacgcc tggctgctca taacgaacgt cagacaaggt gagatcgcgc cagccgccgg
    32521 tgaccttgcc aatatccagt cctggcaccc agcgatccgc cgctttaaat acaagatgca
    32581 ggccgctggt ggtgcccacc agaaacgcca ccgatcccag caacagtaag ataacgataa
    32641 ccacgccgag gctgattttt ttccataaac tcataattct ggccccagac cgatgtaaaa
    32701 ctgtaacccg tgttcgtctt tatccgcgac cggtacggca aaatcgagtt tgattggccc
    32761 gaccggcgat tcccagcgta cgccgacccc ggtaccggtt ttaaagtcgc tgcggcgaat
    32821 atcgcttacc gcttcgccac tatcgacaaa caccgcgccc caccattttc cggtcacgtt
    32881 gtactggtac tccagcgatc cggttatcaa cttcgaggcc cctttcaggt caccgttggc
    32941 gtatttcggg gcgatagatt tgtatttgta gccgcgaata ctgcgatcgc ccccggcgaa
    33001 gaaacgcaga tccggcggta ctttgtcgaa atcaccggtt tcaatccagc ccagcgtgcc
    33061 gcgtgtaaca aaacgatggc gatcgtacag tgtgcggatc cagacgtttt gtgcctggaa
    33121 aacggagaaa tcgacatctg agccccaggc cgtgttggag tagtcgatag agtagcgttg
    33181 cgagtcgccc caggttggca tcaagccacc acgagaacgc gtgcggctaa tcatcacccc
    33241 tggataaaac agcatcgtgg tgttggtaat ttcaccctga gtaaagtggt cgagactcca
    33301 gcgcaggtta atggcacgct gccagccgct ggagagatcc cagtagcgag aagccaccag
    33361 cgtagtggag tcagactcgg tatcgttcag gtcagtgcgc ttaaaaccgc cctgcaccaa
    33421 ataatattgt tccagtggat tcttcagcag cggcatttta tagctgaagt cgagggtctg
    33481 ttccggcgcg gaaatactgg tactggtggt cagactgtga ccataagagt tcatccacgg
    33541 ctttttccac gtcgctttca cgcgcggtcc cacgtccgta gagtaaccga ccccggtttc
    33601 gatggtattt tcagttcgcg gcgaaaccac gcccgtcaat ggtaatactt tcgtttcgcg
    33661 cgctttatca aattgtggag ccactacgac cgagttaaac cagccggtag cagaaagtcg
    33721 acggttcagt tccgccagat ctttcgattc gtactcatcg ccctctttaa acggcaccag
    33781 attttgcagg tattcatcgc ggatttgtga tccttcaaag gtcacatgcc caaagcggta
    33841 acgttcgcca ctgttataat caatatccca gaaggcttta tgcaggccga gcgcaatgcc
    33901 cagctgcgct ttggtaaatt cgctatcgaa ataaccttta cgcaacgcaa tgctggttaa
    33961 ggactttttg aaattttcat aatcgccctg gttcagtacc gtgccaatag ccgggcgagt
    34021 atcgagcatt ttcaaatagt ctttatcggt ccgcgcgccg ccgcgcaata ccacatcggt
    34081 gccgccaatt aacaccggca cgcctggcgt gactttggcg atcaatacct gccgcccttt
    34141 ctttggcggt ggacggagat caaattcaat ggtcggctgg taataaccca gcgctttcag
    34201 accttcgcgg atggcatcat cgacgcgtgc gcgaaagcga cggtctggcg tcacttcatc
    34261 actttcaatc gtagaaagct gcgcacgaac gttcttttcc agctgtcccg ataacccctc
    34321 gacctgtaga cggacgttcg cggcgacggc agatccgctt aagcagagta agcttacaca
    34381 gcataactgt cggatatagc gcacattttc tcctgaatat ccttttttcc tgcccctgga
    34441 acgccgttaa acggcttaac aaaaatccag taatatggat taaaaagcag actaaacccc
    34501 aaatatttct tatgttttac tttagaccta ttcacggtgg ttattgtgtg caaatacgcc
    34561 tcttgttaca accttaaccc caatgaccga ttttcgggag agcgacacca tgagtttatt
    34621 tgataaaaag catctggttt cccccgccga tgccctgcct ggacgtaata ccccgatgcc
    34681 cgtagccacg ctgcatgcgg tcaacggtca ctcaatgacc aatgtacctg acggaatgga
    34741 gattgccatt tttgcgatgg gttgtttctg gggtgtggag cgtctgttct ggcagttacc
    34801 cggcgtttac agcaccgccg caggctatac cggcggctat acgccaaatc cgacttatcg
    34861 ggaagtgtgc tccggtgata cgggtcatgc cgaagcggta cgcattgttt acgatccttc
    34921 cgtcatcagc tatgagcagt tgctacaggt attttgggag aatcacgatc ccgcccaggg
    34981 aatgcgtcag ggcaatgacc acggcacgca gtatcgttca gcgatttatc cgctgacccc
    35041 agaacaggat gccgcagctc gcgccagtct ggaacgtttt caggcggcga tgcttgccgc
    35101 cgatgatgat cgtcacatca ccacggaaat cgctaacgcc acaccgtttt attatgccga
    35161 agatgaccac cagcaatatc tgcataaaaa cccgtatggt tactgtggaa ttggcggaat
    35221 tggcgtctgt ctgccaccgg aagcatagcg ttacgggtac aaatgtagat tgttgataaa
    35281 gtgcgcttta tttatgccag atgcggcgta aacgccttac caggcctaca aaatcgtcca
    35341 aattcaatat attgcaagga ctgcgtaggc ctgatcggca tagcgcatca ggcagtattg
    35401 catttatcag cagtctgaat tttaaccctc tggcgacttt acagtacctt acgctatact
    35461 agccactgaa aatgccggtt cactttcttc gaatcggctt tcaatgtgta tttcacacaa
    35521 attaatcaac ttccccttcc gaggatctgg cctgaaaggt cggataagat atgttaaaca
    35581 gtattttagt catactctgc ttgatcgctg taagtgcgtt cttctcgatg tccgagatct
    35641 cacttgccgc ctcacgcaaa atcaaactta aactgctggc tgatgaaggc aatataaatg
    35701 cccaacgcgt tctgaatatg caggaaaatc ccggcatgtt ctttaccgtg gtccaaatcg
    35761 gtctgaacgc agtggcgatt ctcggcggta tcgtcggtga tgcggcattt tctccagctt
    35821 ttcacagcct gttctcccgc tatatgtcgg cagagctctc tgagcaactg agctttattc
    35881 tctctttctc gttagtgact ggcatgttta tcctgtttgc ggatttaacc ccgaaacgca
    35941 tcggtatgat tgcgccagaa gctgtcgctt tgcgtatcat caacccgatg cgcttctgcc
    36001 tgtacgtttg caccccgctg gtgtggttct tcaacggcct ggcgaacatg atcttccgta
    36061 ttttcaaact gccaatggta cgtaaagatg acatcacttc tgatgacatc tacgcggtag
    36121 tggaagccgg tgcgctggcg ggcgtgttac gtaaacagga acacgagctg attgaaaacg
    36181 tctttgagct ggaatcccgt accgttccgt cttcaatgac accgcgtgaa aacgtgattt
    36241 ggtttgatct ccacgaagat gagcaaagtc tgaagaataa ggtggcggaa catccgcact
    36301 ctaagttcct cgtctgtaat gaagatattg accacatcat cggttatgtc gattctaaag
    36361 acctgctgaa ccgcgtgctg gctaaccaaa gcctggcact gaacagcggc gtacaaattc
    36421 gcaacacgct gattgtgccg gatacgttaa ccctttcaga ggcgttggaa agttttaaaa
    36481 ccgcaggtga agacttcgcg gtgatcatga acgagtacgc gctggtggtg gggatcatca
    36541 ccctcaatga cgtgatgacc acgctgatgg gcgatctagt cggtcagggg ctggaagagc
    36601 agattgtcgc ccgtgatgag aactcatggc tgattgacgg cggcacccca attgacgacg
    36661 tcatgcgcgt gctggatatt gacgagttcc cgcagtcggg caactacgaa accatcggcg
    36721 gctttatgat gtttatgctg cgtaagatcc cgaaacgcac cgattcggtg aaattcgccg
    36781 gctacaaatt tgaagtggtg gatatcgata actaccgcat cgaccagctg ctggtgaccc
    36841 ggatcgacag caaggccacc gccctttcgc caaaactgcc tgacgctaaa gataaagaag
    36901 aaagcgtcgc gtaaccccca gaaacatcaa cggctcctga atcaggagcc gtcttattac
    36961 tgcatagcac tttggttaag ccatctctgt ttgcagacgc ataacctgac ggttaacttc
    37021 ggacatcact gacaaatgca gcttatcctt cacttttggg ataagaatct tacccttatc
    37081 aaattcaaaa gcgccaacgt ccttgatgta taaccgtcca cggaacagga tctttacgta
    37141 cttcgccacc tgaagtgggt tgtaccgttg gaaaattttc attgttatct cctgctgagt
    37201 attacgcctg tgcggtgcca caatcggccc aactattatg aggcgcaaat tttaatgctt
    37261 agtgactata gactatccgg gcaatgtttc caccgcgtat aacttttttt acctaaaggt
    37321 tacaattatt cagaattatc tttttaccga agcgcggtct tcagtataag cattcatttt
    37381 tcatatgatt tgtgcgcttg accgcaaact ggcatcacac ttgcgggaaa ttcgataaat
    37441 agcacatatg attaaaactc agacccaagt ggtcggatca cctgcatatc ataagaagga
    37501 aacaccatga ccctacgcaa gattctggca ctcacctgcc tgctgttgcc gatgatggct
    37561 tccgcacatc agttcgaaac cggtcagcga gtgccgccga ttggcattac cgatcggggc
    37621 gagttggtgc ttgataaaga tcagtttagc tacaaaacct ggaacagcgc gcagttagtg
    37681 ggaaaagtga gagtactgca acatattgct ggtcgcacct cagcaaaaga gaaaaacgcg
    37741 acgctgattg aagcgattaa atcagcgaag ttaccgcacg atcgttacca gaccaccacc
    37801 attgttaaca ccgacgacgc gattccgggt tccggcatgt ttgtgcgcag cagtctggag
    37861 agtaataaaa agctttatcc ctggtcgcag tttattgtcg atagcaatgg cgtcgcgcgc
    37921 ggtgcctggc agctggatga agaaagttcc gctgtagtgg tgctggataa ggatggtcgc
    37981 gtgcaatggg ccaaagacgg ggcgctcaca caggaagagg tgcagcaagt gatggacctg
    38041 ctgcataaat taattaataa atagacactc tgaaccccgg attcaggaac gactcgcgcg
    38101 gagtgtaatc cagcggttta ccctgccagt cgtgaacgtg cgctccggca gccgcagcca
    38161 cagcatgacc agcggcggta tcccaaatat tcgttggccc gaaacgcggg tacagctgcg
    38221 cctgtccttc cgccaccagg cagaatttca gcgaagagcc gatggacgtg gtctgatgtt
    38281 cgccaagctg ttgcagatac tctttcagct ccgcatctgc atgggaacgg ctgataacca
    38341 ccagcggcgg gcgcgcatcg cggacctgaa tctgcttgcg cacaccgcac tcttctttcc
    38401 aggccttgcc ttctgccgcg ctgtacatta cgttcattac cggcgcatac accacaccta
    38461 aaatcggttt gccatggtca atgagcgcaa tgttaacggt gaattcgcca ttacgtttaa
    38521 taaactcttt agtaccatcc agcgggtcta ccagccagta acgctgccag tgctgacgga
    38581 cttcccaacc gggaggatct tcttcagaaa ggaccggaat atccggtgtc agcgtacgta
    38641 aaccgtccat gataacggtg tgtgcggcaa tatccgctgc cgttaccgga gaattgtccg
    38701 ctttgctgac gacgtccatc ggtttcgtcc cgtcgtagac ctgcataatg gcatcgcctg
    38761 cattccgtgc aagctggcat acttgatcta acatttctcc acctcgtctc tgtgagcggt
    38821 gttaacttat tgttttactt ataccctatc gttaatgaat gcgccaactg tgatagtgtc
    38881 atcattttca aagcgtaaaa ttgtggcatt cttcactgtt ctataagtaa gacgtttatt
    38941 ctcccttttc tttcgtattc ccgatgataa aaggatgtcc ctgatgatta agtttagcgc
    39001 aacgctcctg gccacgctga ttgccgccag tgtgaatgca gcgacggtcg atctgcgtat
    39061 catggaaacc actgatctgc atagcaacat gatggatttc gattattaca aagacaccgc
    39121 cacggaaaaa ttcggactgg tacgtacggc aagcctgatt aacgatgccc gcaatgaagt
    39181 gaaaaacagc gtactggtcg ataacggcga tttgattcag gggagtccgc tggccgatta
    39241 catgtcggcg aaaggattaa aagcaggtga tgttcatccg gtttataagg cgctgaatac
    39301 gctggattat acggtcggta cactcggcaa tcatgaattt aactacggtc tggattacct
    39361 gaaaaatgcg ctggcgggag cgaaattccc ttatgtaaat gccaacgtca ttgacgccag
    39421 aaccaaacag ccaatgttta caccgtattt aattaaagac accgaagtgg tcgataaaga
    39481 cggaaaaaaa cagacgctga agattggcta tattggcgtc gtaccgccgc aaatcatggg
    39541 ctgggataaa gctaatttat ccgggaaagt gacggtgaat gatattaccg aaaccgtgcg
    39601 caaatacgtg cctgaaatgc gcgagaaagg tgccgatgtc gttgtcgttc tggcacattc
    39661 cgggctgtct gccgatccgt ataaagtgat ggcggaaaac tcagtttatt acctcagtga
    39721 aattccgggc gttaacgcca ttatgtttgg tcatgctcac gccgttttcc cgggtaaaga
    39781 ttttgctgat atcgaagggg ctgatatcac caaaggcacg ctgaatggtg ttccggcggt
    39841 aatgccgggc atgtggggcg atcatcttgg ggtggtcgac ttacaactca gtaatgacag
    39901 cggtaaatgg caggtgacgc aggcgaaagc ggaagctcgg ccgatttacg acatcgctaa
    39961 taaaaaatcc ctcgcggcgg aagacagcaa gctggtagaa acactcaaag ccgatcacga
    40021 tgccacacgc cagttcgtca gcaagccaat cggtaaatct gccgacaata tgtatagcta
    40081 tctggcgctg gtgcaggacg atccgaccgt gcaagtagtg aacaacgcgc aaaaagcgta
    40141 tgtcgaacat tacattcagg gcgatccgga tctggcaaaa ctgccggtgc tttcagctgc
    40201 cgcaccgttt aaagttggtg gtcgcaaaaa tgacccggca agctatgtgg aggtggaaaa
    40261 aggccagttg accttccgta atgccgccga tctttatctc taccccaata cgctgattgt
    40321 ggtgaaagcc agcggtaaag aggtgaaaga gtggctggag tgctccgccg gacagtttaa
    40381 ccagattgat cctaacagca cgaaaccgca atcactcatc aactgggatg gtttccgcac
    40441 ctataacttt gatgtgattg atggtgtgaa ttatcagatt gatgttaccc agcctgcccg
    40501 ttatgacggc gagtgccaga tgattaatgc caatgcggaa aggattaaga acctgacctt
    40561 taatggcaag ccgattgatc cgaacgccat gttcctcgtt gccaccaata actatcgcgc
    40621 ttacggcggc aaatttgccg gtacgggcga cagccatatc gcttttgctt caccggatga
    40681 gaaccgctcg gtgctggcag cgtggattgc tgatgagtcg aaacgtgcgg gggaaattca
    40741 cccggcggca gataacaact ggcgtttagc accgatagct ggcgataaga aactggatat
    40801 ccgtttcgaa acttctccgt cagataaagc cgcagcgttt attaaagaga aagggcaata
    40861 tccgatgaat aaagtcgcga ccgatgatat cgggtttgcg atttatcagg tggatttgag
    40921 taagtaaaac acttcttttt cggcctataa atcatcaacc gcatccggca tttattggcg
    40981 gatgcgatgc tggcgcatct tatccgccct acaagccatg caccgtagac cagataagct
    41041 cagcgcatcc ggcagttatg ccgcacgttc atcccgcacc gccaacacct cgggcaaatt
    41101 caactcaatc cagtccgcca gtgcagcaac cttttcgctc acctgctcgc ccagcggcgt
    41161 gaggctgtat tccacatgcg gcggcaccac cggatacgcg atacggttaa gaaaaccatc
    41221 ctgttctaac gcctgtaacg actgcgcaag catcttttca ctcaccccac cgattttgcg
    41281 ccgcaggtcg ctaaagcgat gagtaccttc gcgtagcgcc accagaatca atacccccca
    41341 acggctggtg acgtgtttca acacctcgcg cgacgggcac tgttccgcaa agagattacc
    41401 ctctttcagt tgttgcgaca ggctaacctg actcatttca tacttacctt tttgtacgta
    41461 cttactaaaa gtaagtttag gtgttagcgt atttaaacac aagacaaaac gatggagact
    41521 tcccatgatc gctattactg gtgccactgg ccaacttggt cactatgtta ttgaatcctt
    41581 gatgaaaacg gttcctgcca gccaaatagt ggctatcgtt cgtaatccgg caaaagccca
    41641 ggccctggca gcacaaggca ttaccgtgcg tcaggctgac tacggcgatg aagccgcact
    41701 gacatctgca cttcagggag tggaaaaact actgctgatc tcttccagcg aagtgggtca
    41761 acgtgccccg cagcatcgta atgttattaa tgccgcaaag gcggctggcg tgaaatttat
    41821 cgcttatacc agcctgctac atgcagatac ctccccgctc ggcctcgccg atgagcacat
    41881 cgagacggag aaaatgctgg ctgattctgg catcgtttac accctgctgc gcaacggctg
    41941 gtacaccgaa aactacctcg ccagcgcccc ggcagcactg gaacacggcg tatttatcgg
    42001 tgcggcgggc gatggcaaaa tcgcctcagc aacgcgggca gattatgcgg cagctgcggc
    42061 acgcgtgatt agcgaagccg gtcacgaagg caaggtttac gaactggcgg gcgatagtgc
    42121 ctggacgttg acacagttag cggcagagct gaccaaacag agcggaaaat ccgttaccta
    42181 tcaaaatctg agcgaagccg atttcgccgc ggcactgaaa agcgtcggac tgcccgacgg
    42241 actggcggat atgctggcgg attctgacgt tggcgcatcg aaaggtggtc tgtttgataa
    42301 cagcaaaacg cttagcaaat tgattggccg cccaacgaca acgttagccg aaagcgtaag
    42361 ccatcttttt aatgttaata actagttaat taaagtggca tcctcccgca tcctctctga
    42421 taatgacggg atgccgggag caatcatgtc tgcttcctga actttcttct gacagaccaa
    42481 tggatgccag taatgattag cggcgtgctg tacgccctgt tagcagggtt gatgtggggg
    42541 cttatttttg tcgggccgtt gatcgtaccg gaatacccgg cgatgttgca gtcgatgggg
    42601 cgttatctgg cgttagggtt aattgcgctg cccattgcct ggctgggacg cgtgcgtctg
    42661 cgtcagttgg cgcgtcggga ctggcttacc gccttgatgc tcactatgat gggcaacctc
    42721 atttattact tctgccttgc cagtgccatt caacgtactg gcgcgcctgt ttccacgatg
    42781 attatcggca ccctgccggt ggtcattcct gtctttgcca atctgcttta tagccagcgc
    42841 gacggcaaac tcgcgtgggg aaaactcgcc ccggcactga tttgtattgg catcggcctg
    42901 gcgagtgtga atattgctga gttaaaccac ggactccccg attttgactg ggcacgttat
    42961 acctctggca tcgtgctagc gttagtttcc gtggtctgct gggcatggta tgccctgcgc
    43021 aacgcccgct ggctgcggga gaatcccgac aaacatccga tgatgtgggc gacggcgcag
    43081 gcgctggtca cactgccggt ttctctcatc ggctatctcg tcgcctgtta ctggctgaat
    43141 acgcaaacgc cggacttctc cctacctttt ggcccccgtc cgctggtgtt tattagtctg
    43201 atggttgcga tagccgtgct ttgctcatgg gttggcgcac tctgctggaa cgtcgccagc
    43261 cagcgattac cgacagtgat tctcgggccg ctgatcgttt tcgaaacact ggcaggtttg
    43321 ctgtacacct ttttgatacg ccagcaaatg ccgccgctga tgacgctgag cggtatcgcg
    43381 ctgttagtgg ttggcgtggt cattgcagtc agagcaaaac cagaaaaacc cttaactgaa
    43441 tctgtctcag aaagttgaca tactggctgt gagttaaata agcctctgct acgtaagggt
    43501 tatagctttt gccttaaaga tgcatttaaa atacatctta tcttattaag aatgaggtat
    43561 cagctatggc ttatcgcgac caacctttag gtgaactggc gctctctatt cctcgcgctt
    43621 cagctctgtt tcgtaaatat gatatggatt actgctgtgg cggtaagcag acgctgtcgc
    43681 gcgcggcggc acgtaaagaa ctggatgttg aggtcattga agctgaactg gcaaagcttg
    43741 ctgaacaacc gattgagaaa gactggcgta gcgccccgct ggcagaaatc attgaccata
    43801 tcatcgtgcg ctaccacgat cgtcaccgcg agcagctgcc ggagctgatc ctgcaagcga
    43861 ctaaagtcga gcgcgttcac gccgacaaac cgagcgtgcc aaaagggctg acaaaatacc
    43921 tgactatgct gcatgaagag ctttccagcc acatgatgaa agaagagcag atcctcttcc
    43981 cgatgatcaa acaaggcatg ggcagccagg caatggggcc aatcagcgta atggaaagcg
    44041 agcacgatga agcgggcgaa ctgctggaag tgattaaaca caccaccaat aacgtcacac
    44101 cgccgccgga agcctgcacc acctggaaag cgatgtataa cggtattaat gaactgattg
    44161 atgacctgat ggatcacatc agcctggaaa acaatgtact gttcccacgc gcgctggcgg
    44221 gtgagtaatt tgttcgctca acgcacctgg tttacacaat caggtgcgtt ttcaaatgct
    44281 ttgagttatt caccactccg ggacaatctt ttgtatctac tgaactggga aaattcatta
    44341 ttcagccaat aataagttcc ttcaacggtt actaacttat ttttttcatt aatataatta
    44401 cttattcatc gctcctgtat aagccagaat aaaatcaccc attccctggt ttttacgtgt
    44461 acatccagct accgctacgc ttccacccac accgacccgc catcatggcg gttcatcagc
    44521 gaccacagca catggaattt ctcgcctctt ttatactccc cctccatttt cctgtccgtc
    44581 cattctggca tctgctctcc ctgctccagt gtcaccgtcg cttgtctacc ttctacaaaa
    44641 atcgaccact ccccgctatg aggacaagct tcaccgctaa aggccgctat ccgctgccgg
    44701 tctgtaacat actgcgcttc gcgctgtggc cagttgtaca actcctccgg catgccctgt
    44761 gggaaaaatc cctccggcgg cacattgata aatcggtttg ccacccgccg tatacgtttc
    44821 caattgcatt ttgcccacgc cccgctttcc cagtaatatt cttgtatccc agcctcgttg
    44881 acatacttcg ttcgtacccg cccctgccaa gcctcgggaa tatgttcact ccggtacaac
    44941 agttgggcgc tggcatctgg gatatccggc agatacaggc cagccaccgt taccgagtca
    45001 tcctcacgca ggtatacgct actatccagc tcatacaacg gtaattgcat cggcagggcc
    45061 agcgggccgt gcagactttc ctcataataa taagtcagtt cgccgtcata ccagtatccg
    45121 cccgaggtac gtctgattaa attattataa tgtgcacctc gccatttcag tctgtcatac
    45181 tgtttttgcg cggcgagtga catccagtcc gacggataat caattagtcc tttcatatca
    45241 ctgctaatat tgcccccgga ttgcagtacc cgcaggtccc cctgctgtag ctgaataaag
    45301 tcatactgta ccgactttaa agtactgcgg atattgggca acactcgccc gccccagacg
    45361 acgtcaatct gctcgcacag cggacgacgg cgcacatcca gcggcatggt cttgctccac
    45421 tctgccacct gtcgctcgca cagctcgaca aactcctgcc agccctcaaa acgacgccga
    45481 taatattccg gcgacattat tctttccagc aaccagcact cctgtggatg taatatagcc
    45541 actttatctc ctgatttatt aattaattgt ctattgatac ttttatttcg gtacataaat
    45601 atttgtttgc aactgagaca cagctatata catatccact tcatctatca tcggtacgct
    45661 gaccgtcaaa atagacgctg cgtttatcgg cagcgaaagc accgaatgcc tgaaaactgg
    45721 cggcatcgac attaggttca ccgggcggat ttttcaggac tttgccagac caaagaacag
    45781 agcgtccatc ggtgagccag gtatgctggc aattattccg atcatcgcaa tcgaacttat
    45841 cgggattaaa atcggacagt aagcgggttt cttggttatt ggctaaattg gcaatacgcc
    45901 agaggcggga aaaattaacg cccggcagtt cggagaggat ctcatccttt tctcccttct
    45961 gttcggcaac ataaaccgtg cctttctcct gctggcggta ataaaaatct gcgccccagg
    46021 aaggtgaaat ggagagaggt aatgcggcga ttaataacca actgtgctta gccatagtga
    46081 actctgcaat gagtggcaaa gatccacggt aagcccgcgc ctgagagctg tcgatgacgg
    46141 gaagtatttt taggaatgta taaagacaaa ggagattgct gacaaagtgt gctttgttca
    46201 tgccggatgc ggcgtaaacg ccttacccgg cctacaaaag cacgcaaact caatatattg
    46261 cagaggttct gtaggcctga taagcgtagc gcatcaggca attttgcagc ctgaaagaca
    46321 cccgcaggtg ccttagcggc attacttcgc catgcgtttt ttaccaataa acagccagcc
    46381 caaccccagc gcgataaacc acagcggggt aaccagcagc gcctggcgag tgtcatcttc
    46441 cagtgtcagc agcacgatca cgaacacaaa gaacgccata catacccagc acatcagctt
    46501 gccgagcggc atcttgtaga tcgacttctc atgcagatga ggacgctgtt tgcgatacac
    46561 caggtacgag caaaggataa tcgtccagac gaacataaac agaatcgcgg aaaccgtcgt
    46621 gatcatcgtg aacgcgccaa tcacgctggg attcacatac agcatcacca cgccgccgag
    46681 cagacagatg caggagaagg tcagcccttt cgcgggtact gcgcgcttag agagtttagc
    46741 gaacgctttc ggtgcaacac cttcctgggc caggccaaac agcatacggc tggtagagaa
    46801 gacgccgctg ttagcggaag acgccgcaga ggtcagcacc acaaagttga tcacgctggc
    46861 agccgcaggc aaacccacca acacgaacag ttcaacaaac gggcttttct ccgggactac
    46921 cgaactccac ggcgtcacgg acataattac aatcagcgcg aagacgtaga acataatgat
    46981 acggatcgga atagagttaa ttgcacgcgg cagtgatttc tccggatctt tggtttccgc
    47041 agcggttgta cctaccagct caatccctac gaaagcgaaa accgctatct ggaatccggc
    47101 aaagaagcca cttaaacctt tcgggaacca gccgccatca ttccacaaat gcgcgaatga
    47161 cgcttccaca ccagtcggtg actgaaagtg catcgccacc ataaccaggc cgacgacaat
    47221 cagcgacaca atggcgacga ttttgatcat cgcaaaccag aactccatct caccgaacat
    47281 tttcacggtg gcgagattga gcgtcagcag cagcactatc accgccagcg aggcgaccca
    47341 gtcggagaga tcggggaacc agaactgagc ataagccgtg atcgccacca cgtctgccat
    47401 accggttaca acccagcaga accagtaagt ccagccggtg aaatatcctg cccacggccc
    47461 gagtaaatcg gaagcgaagt cactaaaaga tttgtattcc agattcgaaa gcagcaattc
    47521 ccccattgcc cgcatcacga aaaagagcat aaaaccgatg atcatataaa cgaaaatgat
    47581 cgacggcccg gcaaggctaa tcgttttgcc agaccccata aacaaccccg taccaatggc
    47641 accgccaatg gcaataagct gaatatgtcg gtttgtgaga ttgcgccgta gcgactgttc
    47701 agccggagcc tgatcatcgg caacgacttt tacctgatct accatgtttt tttcttcctg
    47761 tacctgtctg tgttgttcag gctctacggc ctttagtcag tctatgatac gacgaatctg
    47821 tatcgggact catcgatatt aggtaacaat acgcgggatg aatactaaga tttagatcta
    47881 atgttaattt tatgtttaaa gtgagtgcca tatcacggtg ataacgcgaa acagctcaca
    47941 aaaatacacg cttacggggt ctttgcaaca caaaatagcg ccgtttggca aactgaaggg
    48001 tttattgctg aatgcctgct cccctctggt tcgaggggaa cagaatgcgc tgcttagagg
    48061 atttccagca gttcgacttc aaacaccagg gtgctgaacg gagggatgga tgcgcctgcg
    48121 ccgcgctcgc catatgccag ttcctgcggg atagtcagtt cccatttaga acctaccggc
    48181 atcagagtca gtgcttcaat ccagccaggg atcacgccat taaccggaaa ttcagcgggt
    48241 tcaccacgag caacggagct gtcaaacacg gtgccgtcga tcagtttacc ggtgtaatga
    48301 acacgaacgc ggtcggtacg tgccggaatt gcgccttcac cctggttgat cacgcggaat
    48361 tgcaggccag attcggtgct attcacacct tcttttttgg cgttttcttc caggtatttc
    48421 acaccttcag cagccatcgc ctggaaacgc tgacgacgaa cggcatcggc gcgctcgtgg
    48481 atttcacgca gcgcgcgatg caccacatca accggaacag ccggatgttt gccttccagc
    48541 gcatcggcaa tacctgcaac cagtgcttct ggcagcagcc cttccaggcc agattcactc
    48601 agttgttgcc ctacctgcaa accaatgccg tagcttgctt gcgcttcgat ggtgtcaaaa
    48661 gttggggtgg tcatcgtatt tcctttctta gggtataaaa gtagcgggca gcatatcagc
    48721 cacacttctt tgggtaaaac tttgtcgcag gaatgatgac aaatcccggg gaaaaagaaa
    48781 caatagcaat atcccgtatg aatataataa aggcgggttt accacggaca tcatagcgtt
    48841 agcttattca taggctatga ttgaggaaca agacgcggag caggaggaaa ccatgcccgg
    48901 gcgctttgaa ctaaaaccaa ccctggagaa agtctggcac gcgccggaca attttcgctt
    48961 catggacccg ctgccgccga tgcatcgtcg ggggatcatt attgccgcca ttgtattggt
    49021 ggtcggattt ctgcttccat ctgatgatac gcccaacgcg ccggtggtga cgcgtgaagc
    49081 gcagttagat attcagtcgc aatcacaacc gccgacagaa gaacagctgc gggcgcaatt
    49141 agtcacccca caaaatgatc ctgatcaggt ggctccggtc gctccagaac ctatacagga
    49201 aggccagcca gaagagcagc cgcaaactac ccaaacgcaa ccattccagc cagatagcgg
    49261 aattgataat caatggcgct cttaccgtgt cgaacccggt aaaacgatgg cgcagctgtt
    49321 ccgcgaccac ggactgcccg ctaccgatgt ctatgcaatg gcgcaggtag aaggcgcggg
    49381 taagccgctg agtaatttgc aaaacgggca aatggtgaaa atccgccaga acgccagcgg
    49441 cgtggtgacg ggcttaacca ttgatacggg gaataatcaa caggtcctgt ttacccgcca
    49501 gccggatggc agttttattc gtgcgcggta gatgaagggt caaccaaaag ccagctaagg
    49561 attgtctctt tgatgacctt acgtctggct tccagtgcct ggggggaaac catactgata
    49621 tgataaacca ggccaagcgt atgctggttg atcaaataat atcccccaag cgcagcaata
    49681 ttaatattca cctgcaatgg gtcgatatct ggcttaaaaa tattgtgttt cttcccttca
    49741 tccaataagg attccattaa ctgcaagtgc gcatggttaa tttctaagag acgctgtgat
    49801 tttgcataat gcacaccttt actttgattt tcactatgca caatttttaa aaaccagggg
    49861 ttagcaatat aataatccca tgtaaaatca ataattttct caatagcttt ttgtggcgga
    49921 agatctgaga aatcaagggc cttttctttt tgtcgaatat tgttccaggt atattccagt
    49981 acttcaataa ataaattttc tttattacca aagtgatgat aaaccgtctg tttactacac
    50041 cccgcagcct taacaatatt atcgaccctg gccccttcat aaccatattc ggcaaattca
    50101 ttaaccgcgc ataacagcag cttttccttc aggctctgta cgttgctggt atcaagatac
    50161 ataacgcttc cccacgacct cttcaagata actatcttaa cccgattaat tagaagctcc
    50221 agtaaaaagg ataaaaactt gtactttcat caagttatca aatgtactgt gatcactatc
    50281 gcatatcaac caaccagatg gttgatcttt cttgcggcac gtcgcaagct aattgtcact
    50341 tagaaaacaa tattcaaaaa aagagagcta ttgtatgaaa tccactcgtc ctgttgccgt
    50401 gattaccgga gccgctcgtg gtattggaaa gggctgtgcg ctggagcttg ctcgtggcgg
    50461 atttaacctg ctgattaacg atcttcccga tgccgacagc gtagaaaaac tgcatatcac
    50521 acaacaagaa tgtatagctg aaggcgtgga agtgatttgc ttccctgccg atgtcggcga
    50581 tctctccttg catgaagaga tgctcgacgc ggcgcaaaac cagtggggac gtctggactg
    50641 cctgctcaac aacgcaggca tttcagtgaa aaaacgcggt gaccttctcg acctcgaacc
    50701 agacagcttc gaccaaaata ttgccattaa cacccgcgcg ccgttcttcc tcgctcaggc
    50761 attcagtaaa cgtctgctcg cacagccgaa accggaagca gaactgccgc accgctcgat
    50821 tattttcgtc agttccatca acgccatcat gctggcgatg aatcgcggcg aatataccat
    50881 tgccaaaacc gccgtttctg ccgctgctcg tctgtttgct gctcgccttt gtaatgaaca
    50941 aattggcgtt tatgaagtgc gccccggtct catcaaaacc gatatgacta ttccggcaac
    51001 ggcttattac gacgaactga ttgccaaagg tctggtgccg tggggacgtt ggggttatcc
    51061 ggctgatatt gcttccactg tccgcgcgat ggcggaaggt aaactgattt acacctgtgg
    51121 tcaggcggtc gccatcgacg gtggcttaag tatgccgcgc ttctgaggct gaatcatgag
    51181 agatctgtta caacgcccgg atttattcag tatcaatacc gcaacactgg gctataaaac
    51241 gccgctaccc gccattattg atgcctgcgc cgcgcgtggt attggcgcga ttgctccctg
    51301 gcgcagggag ttgcaaggtg aagatctcca gcaaatcgcc caccagcttg ccgccagtaa
    51361 catgagcgtt tccggtttgt gccgtagcac ttactacacc gcgccgacac tggcggaacg
    51421 caaactggcg attgatgata accgccgggc gctggacgac gcagcggtgc ttaacgccgc
    51481 ctgttatatg caggtggttg gcggtctgcc aacgggcaca aaagatctgt acgaagcgcg
    51541 ggaacaagta aaacaaggga ttcgccagtt gctgccacat tcgaaagatg tcggcgtgcc
    51601 aattgcgctg gagccactgc acccgatgac agctgccgat cgctcttgcc tgtgcacgtt
    51661 acgccaggcg ctggactggt gtgatgaact cgatcccgat ggtgagtttg gcctcggcgt
    51721 ggcggtggat gtttatcacg tctggtggga tccggatctc gccagtcaga tcctgcgtgc
    51781 cggaaaacgc atcctcgcgt ttcatgtttc cgactggtta gtgccgacca ctgatctggt
    51841 caatgaccgt ggtatgccgg gagatggcgt catcaatatt ccgtcaattc gtcggctggt
    51901 tgaaaacgcc ggatttaatg gtgctattga actggaaata ttctcgcctt actggtggca
    51961 gaaagatatt aacagcacgc tggatattag cgtcgatcgc atcgcgcatt attgttaagg
    52021 aatgatcatg agtgaccaac ccgttctgtt cagcagagtg gcggcttcct gccgcctgac
    52081 attgaaccgt gaagataaat gtcatgctat taacgaagag atgatcgaat ctctcgacca
    52141 ttatttaaat gaaattgaaa acgacaccac attacgatta gtcgaattaa cggcaaccgg
    52201 cgataaattc ttttgtgcgg gcggcgatat taaatcgtgg tcggcctatt cgccattaga
    52261 tatgggcaga aaatggatta aacgcggtaa tgacgttttt aatcgcctgc gaaacttacc
    52321 ccaattaacc gttgcgaatc tcaacggtca taccatcggc ggtggtattg aactggcctt
    52381 gtgctgcgat attcgtattg cccgccccgg cgcgaaattc tctaacccgg aagtgatgct
    52441 cggcatggtg cctggctgga tgggcattga acgtgtgctt aatcaggttg gtccggtggt
    52501 cggtcgccag atgctgatgc tgggtaaacg cctgacggcg caggaagccc aggcggcgaa
    52561 tttaattgat gaagtggtag aaatagaaca ggtggaaagc tggatggcga accagttagc
    52621 gcagctggaa aaatgcggtc cggtggcact ggcgcatatt aagcaactga ttctggcact
    52681 ggaaaataaa catgccgatt acccgcacca gctactggcc ggattaatgt ccgccactca
    52741 ggattgccag caggcgacgc gcgcctttgc cgaaaaaagt agcgtgagct tccataatca
    52801 ataaggaaat tgtatgcgta agataacaac cgccgaggca ctggcggcgc aaattcagga
    52861 cggcgcaacc attgccatta gcggtaacgg cggcggcatg gtggaagccg accatattct
    52921 ggctgctatt gaagcgcgtt tcctgcaaac cggacaccct cgcgacctga cgctaatcca
    52981 ctcgctgggt attggcgatc gcgactgcaa aggcactaac cgttttgcgc acgcagagat
    53041 gctcaaacgc attattgccg ggcactttac ctggtcgccc aaaatgcagg cgctggtaaa
    53101 aaataacacg attgaagcct actgctttcc tggtggcgtg attcaggcac tgctacggga
    53161 gatcggcgcc ggacgtccgg ggctttttac ccacgttggg ctgggatcgt ttgttgatcc
    53221 acgcaatggc ggcggtaagt cgaatgaatg cactaccgac gacctggtag aactgattga
    53281 aatcgacggt gaaaccaaac ttcgttatcg ccctttcaaa gtggattacg cgattttgcg
    53341 tggcacttat gccgatcctc gaggcaacgt cagccttgaa gaagaagcga ttgatatgga
    53401 tagctattcc atggcgctgg cagcacacaa cagcggcggc aaagtgttcg tacaggtacg
    53461 cgatgtgctg gaagctggcg cgattgaacc acgccgggtc aaattaccgg gcattctggt
    53521 tgatggcatc gttgaacacc gcgaacaacc acaaacctat cttggcggtt acgacctgac
    53581 catcagcggc caacatcgcc gtctgagttc taacgacgcc attgaactgg ttagtcatcc
    53641 ggtacgtcgc ctgattgccc gtcgggcggc acgcgaactg gtagctggcg cttcaaccaa
    53701 ctttggcttt ggtattcctg ggggtattcc aggcgtagcg ctgcgcgaag gcgtgcctta
    53761 ccaaagttta tggctgagcg tcgagcaagg cgtgcataac ggtatgatgc tggatgatgc
    53821 gtttttcggc tgcgcccgca acgccgatgc cattattcca tcactcgatc aatttgagtt
    53881 ctacagtggc ggagggatcg atatcacctt cctcggcatg ggagagatgg atcagtacgg
    53941 taacgtcaac gtatcccatc tcaatggcaa tctcattggt cccggcggtt ttctcgaaat
    54001 tgcgcaaaac gcccgtaaag tggtgttctg cggcacgttc gacgccaaag gtagcaagat
    54061 tgatgtaacg ccagatggct tgcatatcac ccagtcaggt caaatcccta aactggttac
    54121 ccaggtggaa aaaatcactt ttagcgccgc ctacgcacag caaagtggtc aggaagtgtt
    54181 gtatatcact gaacgcgcag tattccagtt aacggcagaa ggcgttgaat taattgaaat
    54241 cgcgccgggt gtggagattg agcgcgacat tctgccgtat atggctttcc gtccaattat
    54301 caagcaccca cgcctgatgg aaagtagcct gtttacgccg atggaggatg catgagtcag
    54361 cttgatgaca ccatccttga tgcgctgacg cacgttactt tcccaaaggg ttttgcacag
    54421 gcagagcccg catgggttgt cacggtagac ggtgttgatt acccactctg gcaaacagat
    54481 gccctggtag tcggcagtgg tgcagcagga ttacgtgcgg cagttgaact taaacgccgc
    54541 cagcaaaatg tgctgatcgc taccgccggg ttatatatgg ggacgtcggc atgttctggt
    54601 tccgataaac agacactatt taccgccgct accgcgggca acggcgacaa cttcaccaaa
    54661 ctggcagagg cactggcgag cggtggggcg atggatcacg acaccgctta tgtcgaagcg
    54721 gtaggttctc tgcacactct aggcgggttg caatatcttg gtctggaatt accggaagat
    54781 cgctatggcg cgattcttcg ttatcaaacc gaccatgacg aagccgggcg tgcaacctcg
    54841 tgtgggccgc gtacctcaag gttaatggtg aaagtgctgt tggaagaagt acagcgcctc
    54901 gccattccag tgttgaccag tgcaacagtg attaaactgc tgcatcagcg tgacgaaaac
    54961 ggcgaagacc gcgtggcagg ggcaatcctc gcgaccggtc atcgcgccca taacccatgg
    55021 gggctggcaa ttgtgactgc gcccaatgtg gtactggcaa caggagggcc tggcgagctt
    55081 tatcgcgaca gtgtttatcc acataaatgc ttcggctcgc tggggctggc gctggaggaa
    55141 ggtctgacgc taaccaatct gaccgaaagc cagtttggta taggcacgcc gcgtagcacg
    55201 tttccgtgga atttatccgg cacctatgta caggtgatcc cgtatatcta ttccgtggat
    55261 gctgagggta acgagtataa cttcctcgcg gattactatc gcaccaccca ggagctggct
    55321 tcaaacattt tccgtaaagg ctatcagtgg ccgttccacg ccacgcgggt gatggatttt
    55381 ggctccagct tgttagatat ggcagtagcg caagagcagc aatcagggcg tcaggtattt
    55441 atggatttca atcgcaatcc tgaagcggtg ccgggtgacc tgccgttctc attagatcga
    55501 ctggacgacg acgttcgcgc gtatctggaa aataacgacg ctctggcacc atcgcccatc
    55561 gaacggctgc aacgaatgaa tccgctgtct atctcgctgt ataagatgca cggttacgat
    55621 ctcaccacac agcctttgca gtttgccatg aataatcagc atatgaatgg cggcattgaa
    55681 gtggatatct ggggacaaac ctccctgccc ggctgtttcg ccgtggggga agttgctggc
    55741 acacacggcg tcactcgccc tggtggtgca gcattgaatg ccgggcaggt ttttgctgtt
    55801 cgtctggcac gttttattgg ttgcacgcaa aagcggaata ttgatggaga tatagcacag
    55861 ctggtagctc ctacactggc ttctataaga gagatcatta ctcaggcgca cgataacggg
    55921 accgggatgc cgttgtcggt tgtgagagaa aaaattcagg cacgaatgtc tgaccatgcg
    55981 ggatttattt gccatgccga taaagtccga cgcgccactc gcgatgccct gctattgagc
    56041 gaatttgtcc aacggcatgg attggctatc aaacatgtgg gtgaagttgc cgagctgttt
    56101 atgtggcggc atatggcact gacttctgcc gccgtattaa cgcaattaac gcattatatt
    56161 gacgcaggtg gtggtagtcg gggagcacgt attattctcg acagggatgg aaattctatt
    56221 ccgcaaacac gaaatggttt ttgtgatgcc tggcgctttc gttcagagcg tactgaggat
    56281 aaaaaagata aactacttat tcattattgc aacggtatct ttcacgtcag agaaacacct
    56341 gttcgtgagt ttcctattat ccgaggtatt tggtttgaaa aaaactggcc tggttttctt
    56401 aatgggacaa tttaccagcc acaggatgaa tgatattgca aaggttaatc actatgcgaa
    56461 atataattag atctattaat gctctgcttg cagcattgaa cattaccatt ttggcgatca
    56521 tcgtggcatg tgtgacgtgg caggtggctg cacgatttat atttactagc cacagtattt
    56581 ttacagatga actcagccgt ttgctcttta tttgtcttgg actttttggt ggggcataca
    56641 ccgcagggca aaaccggcat ctggcgattg acctgctacc tatgatgctg aaaggaaaag
    56701 cccgacgtca tttatttttg tgtattcaga ttatcgttat tatcttcgca accatcatta
    56761 tggtatatgg tggtgggcta ttaacgatgg atacattcga ttcaggtcag acctcgcccg
    56821 ctttaggctg gcaaatggga tatatctata tgagcatccc gattagtggt gtacttatta
    56881 ttatatacac catagatatg gttttaaccg aacttaaaca accactctaa agataacaaa
    56941 caacaaccaa ccatagtagg aatggagaaa ttatggatta ctggttaccg attattgtgt
    57001 tatttggtgc cttctttttt atgttggcat taggtgtacc tattgtttat gccattggtt
    57061 tatcgacact ggcatcaatt tccacacaac tcgatttcaa ctcagcattg agtgtggttt
    57121 cccaaaagtt agcctccggg ttagatagtt ttacgttgtt ggcaatccca ttttttatct
    57181 tgtctggcaa cattatgaac catggaggaa tagcgcgacg attaatcaac tttgcccgta
    57241 ttctcggcgg tcgtctgcca ggctcacttg cccactgtaa tattctcgcc aatatgctgt
    57301 tcggggcaat ttctggttca gccgtggctt cggcggctgc gatgggcggc gtcatgcatc
    57361 cgcaacaggt taaagagggt tacgacccgg cattcagtac tgcggttaac gttgcctctg
    57421 ccccgacggg tttgcttatt ccaccaagta atacgttaat cgtctattca ctggtcagtg
    57481 gtggtacttc tattgccgca ctgttcctgg cgggttatgt tccgggaatt ttgctgggtc
    57541 tggcactgat ggtaatagcc ggtattatcg cggtacgtcg cggttatcca aagccagaac
    57601 gcccgacttt acgccaggct ggcgttgcga tatggatggc tatccccagt attttcctga
    57661 ttattttaat tatggggggt gtattaagtg gtatttttac cccgacagag gcctccgcaa
    57721 ttgcagtaat ttataccctt ttccttgcac tggttcttta tcgtgaaatc tcagttaaag
    57781 atttacctaa aatatttctg gaatccgtta ttaccactgc aattgtcttg ttattaattg
    57841 gttcttcgat gggtatgtca tgggcaatgt caaacgctga cgttcccttc ctgattctcg
    57901 atttgcttaa cactatttct gacaacccaa taataatcct gcttattatc aatattatcc
    57961 tgttgattat cggcactttt atggatatga caccggcggt actgattttt acgccgatat
    58021 ttttaccggt tgtcaccgaa ctgggcatgg acccgattca ctttggtatt gtcatggtac
    58081 tgaacatgtg tatcggtatt tgtactccgc ctgtaggcag tgtgttattt gtcggttgta
    58141 gtgtatctaa attaccgatt aataaaatta tcaaacctat gctgccattc tatgccgtga
    58201 tggtattggt gctggcaatg gtgacctata ttccgcaaat cagtatggca ttgccaagag
    58261 cactgggcta ctgatgcgca ttcactggcg gcacctgccg ccatatttat tagaaaagga
    58321 acatctatga aaataaaagt atccgctggc attatcggtg ctgttcttat gttatccgca
    58381 agccagtcct gggcagtgac attaaaactg agtcataatc aggataagtc tcatcctgtt
    58441 cataaagcga tggagttctt tgcgaaaaag agcaaagagt actctaacgg tgatattact
    58501 attcgtattt atccaaatgg aacattgggt actcaacgag aaacaatgga gctgattcgt
    58561 tctggcgcta ttcccctggt aaaaaccaat gcggcagaaa tggaagcatt tgaaaattcc
    58621 tataaattat ttagcctgcc ttatttgttc cgcgatcgtg atcattatta tcaggtcatg
    58681 cagggcgata tcgggagaaa aatcctcgac tcaacgaaaa gcaaaggtta tttcgggctg
    58741 actttttatg atggaggcgc ccgcagtttc tatggcaata aaccagtact gaaaccagac
    58801 gatctgaaag gcatgaaagt ccgtgtccag ccaagccctg gcgcagttga aatgatcaaa
    58861 gtcatgggcg gtaacccgac gccactggat tacggcgagt tgtatacagc cttacagcag
    58921 ggtgtggtcg atatggcaga aaacagcgtg atggcgctga ctaccatgcg tcacggtgaa
    58981 gtggcaaaat ccttcagcct tgacgaacac actatggttc ccgatgtggt tctgatgagc
    59041 aatgctgcgt ttgataaact tagcccggaa aatcaggcag ttatattaaa agcagctaaa
    59101 gaatcaatga gctacatgaa agacttgtgg agcgaggaag agaaacaaga atttgcaaaa
    59161 ctggataaaa tgggcgtgaa agtctaccag gtagataaag ctccgtttat cgagaaagta
    59221 cagccaatgt acgcaaactt cgctaaggac aacccagccc ttgccccaat gctggctgat
    59281 attcaggcag ctaagtaatt atcttcgtag ggctgcttat tgtggccctt atcgatatct
    59341 gcaagtgctc gctacaaaag caaaacgccg accaatggtc ggcgttttta cgtctcgttg
    59401 aataacgaat tattcagcta ctacgtttac gatcactttc gcgaatactt cgctgtgaac
    59461 ctggaagctc acttcgtgtt cgccagtggt acgcagaacg ccgttcggca gacgaacttc
    59521 gctcttagcc acttcaacgc cagctgcagt tacagcgtca gcgatgtcgc gagtaccgat
    59581 ggaaccgaac agtttacctt cgtcgccagc tttagacgcg atggtaacag tttccagtgc
    59641 attgattttc tcagcgcgag cattagcagc tgccagaact tcagccagtt tagcttccag
    59701 ttcagcgcga cgtgcttcga agaattcaat gtttttcttg gtagctggaa cagctttacc
    59761 ctgcggtacc aggaagttac gagcatagcc cgctttaacg tttacctgat cacccaggct
    59821 acccaggttt gctactttat caagcagaat aacttgcatt accttatcct ctcaaagtcg
    59881 tattaatgga ccgtgaccga ttactgatgg cgatcagtgt acggcagcag ggacaggtag
    59941 cgagcgcgtt tgatagcgcg agccagctga cgctggtatt ttgcacgggt accggtgata
    60001 cggcttggga caatcttacc gctttcggtg atgtagtttt tcagcgtagc gatatcttta
    60061 tagtcgatct cttgaacgcc ttccgcggtg aaacggcaga acttgcgacg acggaaataa
    60121 cgtgccatat ggctagtctc cagaatctat caattcaatc tgctcggcat gcaaaaccat
    60181 tttgctcagt ccgttctttg ccttgtggca tgaaatgaac ccctgaacgg ttatgcgact
    60241 gccgaccgtt atactgtgag taatgacctg gttttcgtgt ccgctaacaa taacgggcat
    60301 ttgacaccat gcctgccggt gaaagccggc ttcctcctgc acagaacgat gctcaagcac
    60361 gaactggcag tgaggaattc ctgatggact gacctttcga aggggagtcc tgcacacggt
    60421 gccggacaac accagacggt tggtcatcag aaattactct tcagaatccc cagcttcagc
    60481 atcatcagcg gtttcgtttg cgaaatcatc gcgacgctca cggcgctcgt ctttcgcttt
    60541 aaccatcgga gatgcttcgg taacagcgtg cttggtacgc ataaccatgc tgcggataac
    60601 ggcatcgttg aagcggaagg tagtttccag ctcatcgatc acttcctgcg gagcttcaac
    60661 gttcatcaga acgtagtgtg ctttgtgcag tttgttgatc gggtaagcca gctgacggcg
    60721 gccccagtct tccagacggt ggatcttgcc ttctgcacca gtgatggcag cagtgtagcg
    60781 ctcgatcatg cccggaacct gttcgctctg gtcaggatgg accataaaaa cgatttcgta
    60841 atgacgcatc gaattgctcc ttacggatta ttcagcctcc tgtctgggtc agccgaatcc
    60901 cggggaggca aggaacgtgt taaaggtcgg ctgaaaaatg acgcgttata gtacttgtat
    60961 gccccgctaa actcaaggcc agccgacaaa taaatctcat ctttcgctca aaagaactaa
    61021 ctccctgatt tgaagttcat cacattccgt cacagtgttt tttcgaacaa ctgcatcaga
    61081 aatggtcacc atcaacttct ttatcattga ttaaaattaa atcagaagat catcacagag
    61141 gagagcacac aaagtcagcc gtcgaggagg tatctatgtt cagtcgtgtt ttagcccttc
    61201 tggctgtgct tttgctaagt gcaaatacat gggcagccat tgaaatcaat aaccaccagg
    61261 ccagaaatat ggacgatgtg caaagcttag gcgtgattta tatcaatcat aatttcgcca
    61321 ctgaaagtga agcacgtcag gcattaaatg aagaaacaga tgcgcagggc gcaacgtact
    61381 accacgtaat tctgatgcgg gaaccgggga gtaacggcaa tatgcacgcc agcgcggata
    61441 tttatcgcta gcaccaggta taaccaacga aacattgcca tagtttgctt tgcccccttc
    61501 gcagggggct ttttttgaaa aacggctgcg gaattagacc agttatctcc cgaagaagga
    61561 aatttccgca gcgcgtgttt tacttctgcc cgtaataagc gttaggacca tgcttacgca
    61621 tgaagtgttt attcatcagg aagctgtcga tgtgattgag ttgtggatta atactgcgcg
    61681 caatccacgc cattttcgcc acttcttcca tcactaccgc gttatgcacc gcgtcgtggg
    61741 catctttccc ccaggcgaac ggtccgtgct gatacaccac aattcccggc gtatgcagcg
    61801 gctcggcgtt gcccagcgtt tcgataatca ctttgccagt gttcagttca tactcaccct
    61861 gcacctcttc ttcgcttaat ccacgcgtac acgggatgtc accaaagaag tagtcggcgt
    61921 gcgtggtgcc taacgccggg atcgccagcc ccgcctgcgc ccatgcggtg gcatgagtgg
    61981 aatgggtatg gacaatgccg ccgagcgacg ggtaacgacg gtagagttca agatgcgtcg
    62041 cggtgtcaga agacgggcga tacttccctt ctaccacctt gccgctcata tcgaccacca
    62101 ccatatcgtc tgctttcatg gtttcgtagg caacgccgct gggcttgatc accaccagcc
    62161 cgcgttcgcg gtcgatagcg ctgacgttgc cccaggtaaa ggtcaccagc ccatagcgcg
    62221 gcagatccat gttggcttca aatacctgct gttttagctt ttgcattatg ccgcctccac
    62281 catgcccgct ttcgccatgc gcgctttcac ccaatcacgc gctttcgcca cttctgccgc
    62341 cgggtcttcc gccgtttcgc tccacatctc aatcaggtac ggcccgcaat agccactctg
    62401 tttgagcgtt tcgaaacaac gttcgaaatc cactacacct tcgccaaacg gcacgttttt
    62461 gaagacgcca ggtttggtgt ctttcacatg taccgcgacg atatgcccga ttccggcctg
    62521 caactccatc tgcacatcgt tgtcccacgc cgacaggttg ccgatatccg ggtagagctg
    62581 gaaccacggg ttgttgagat agtgcgcgta acccagcgcc ttgctgatgg agttcatcaa
    62641 cggataatcc atgatctcca tcgccagcgt cacctgcgcg cggctcgcca tctcaacgct
    62701 ctctttcagg ccgtcacgga aacgacgacg cgtttcgtta ttggcttcct gatagtaaac
    62761 gtcatagccc gccagctgga tcacgcgaat gccgacgtcc tgggcgaact ggatagcttt
    62821 acgcataatc tccagcccct gcgcccgcac cgcgtcatct tcactgccca gcgggaaacg
    62881 acgatgagca gaaaggcaca tggacggcac gcgcacgccg gtttcaacaa tcgcattcac
    62941 cagcgccaga cgctgctcgc ggctccagtc gaggcgcgac aggcgctcgt ccgtttcatc
    63001 taccgacatc tcgacaaaat cgaagcccaa cgtttttgcc agttgcaggc gttccagcca
    63061 gcactccccg gcggggagcg ctttttcata gatgccaagc gggatttgtt tggacaacat
    63121 atccgctcct tagccccaca gttcggcgat ggaacgtttg aactgacgtg cagcttcaac
    63181 cggagaagcg gcatcacgga tgctgcgacc agcgataaac acatgaatcg gaataccttt
    63241 gaacagcggc agatcttcca gcgccaggcc accggtgacg gtgactttga agcccatatc
    63301 ggaaagacgt ttgatcgcgg taatgtccgc ttcgccccac gctacgcctg cggcctgcgc
    63361 gtcacggctg cggtgataaa ccacctgctg aatgcctgcg tcacgccact gctgcgcctg
    63421 ttcccaggtc cagtagccgg tcaattcgat ctgcacgtcg ccgttaaact cttttgctac
    63481 gtccagcgcg cctttggcgg tgttgatatc cgcacagcaa attaccgtca cccagtcagc
    63541 gttggcttcg aagcacatac gcgaaaggat tttgcccgca tcagcaattt tagcgtctgc
    63601 cagtacgatt ttgtgcgggt agagcgcttt caggtcacga accgcacgca cgccttcgcc
    63661 cacgcacaga atggtaccca cttcgataat gtcgacttct tcggcaatca ggcgagtggt
    63721 ttcgtaggcg ctatccatag tctggttgtc cagcgcgact tgcaacatcg gtaatgacat
    63781 cttcaattcc ttcttaagct gccgcgttgg tgcggtcaat gagatccagt acttcctgct
    63841 cggtacggca ggcacgtaaa cggtcgaaat tttcttcatc ttcaaacagg ttgacgatct
    63901 gcatgatgcc cacttcctgg tgagtgttgg catcgaccgc cgccatggtg atgagtatat
    63961 ccaccgggtc attatcttcg tggttgaact ccagcggctt tttcagcgtc accagcgcaa
    64021 aaccggtttt cttaacaccc tcttccgggc gaccgtgcgg cattgccagg cctggtgcga
    64081 gcacaaagta cggaccgtgc tgttcaaccg catccagaat cgcctggtag taacgcggct
    64141 cgacaacatc cgccgccacc agcaggtcaa cgccgatttt tactgcgtcc tgccatgtct
    64201 cagcttcagc ctgcaggcgg atggatttat tctccgccag cgaatcacgt aatttcatgg
    64261 tgcgtcctta cttcacatcc tgcgggaaat gctctttgat cacttccagc agtttcgggc
    64321 caaagtcagc aggagagagc atgttgcgca cgccaaccac gtatttgttg ccggtcacgg
    64381 tgatttcgcc cgcaatgtgc gtagaagcga tgatgatatc cgcgccgctc aactcgcttt
    64441 tgtattcgcc aaccgcgcag ctgtttaccg tgtggtcaat gtttgattgg gttaaaaact
    64501 ggtccacttt catcttcatg atcatggaac tgccttgtcc gttgccacac acagccagaa
    64561 tacgtacggt cataatcaaa actccttatt aagcagactg ttctgccagt tgtttttctg
    64621 catcttcttc tgcgcgcagt gcgcggccag cgaagaacat ataagccagt gcaattacaa
    64681 tgatgacggc cataaaggcg ataccgatgg agaagaagcc ctgcatcatc ggcggtgcca
    64741 gaatcgacca gtccgccata cccatccagg cactcatacc ggtaagtttc accgcccaga
    64801 cgcaaccaaa gatttcgatc atccccatca ccagacaaat cttcagcgcc gcacgccagc
    64861 cgccgaagtg gttagcgaac acgccgatgg tggcgttgga gaagaacatc gggataaagc
    64921 caggaataat caggattgag gaaccgcagg cgaccaggat gccaaccgca atcagctgac
    64981 cgatggtgcc ccacataaac ccccagacca cggcgttcgg cgcgaagcta tagatagccg
    65041 cacagtcaat cgccagaacc gcacctggga tcaggcgctg ggaaatgccg ttaaatgctt
    65101 cagagagttc cgccacaaac atgcgcacac cctgagtgat gatgaagatc gccaccgcga
    65161 aggagaaacc agtttgcagg atgtacaccg tccagttcac tttgcctgcc atcgcctgca
    65221 cggtgtcgat accgaaggag agcagaatgg caccaaagaa gatggtcatc acaatcgccg
    65281 tggagacgat gttgtcgtgg aaaatattca gccagcctgg cagtttgagg tcttcaacgc
    65341 tttcctcttt tttgccgagg aacggcgcaa ctttataggc aatccatgat gcaaactgtt
    65401 gctggtgacc gatggagaaa ccgcagccgt ccgtcacttc ctgagtcggc ttgtacatta
    65461 tgttggaggt aatgccccag tagagcgaaa ccagaatcgc cgtgcagata atggtcgtcc
    65521 acatggagta gccgaaaata aacaacgtaa cggcaatcag ccccgcctgc tggaacatga
    65581 tatgaccagt cagcatgatg gtacgaatac cggtaatgcg acgcagcagc acgtaacaga
    65641 tgttcaacgc cagcgctaac aacaccgcgt aacctaccca gctataggca tcgcccatgc
    65701 ggtcgatggt tgccatcatc gacgcgtaag tatcggaaat tgcgccgtta atgccgtaga
    65761 cttcggacat tttcgccacc accggtttga aggtgctggt gaggatgccg gaccctgcct
    65821 gcaacaacat gaaaccaatt atggttttaa tcgtgccttt aataataacg ctgacacttt
    65881 tgcgcagtag gatgtagccc agacaggtca caatacccag caacaacggg gcattggtca
    65941 tgacctggtt aaaaaacacg gtaaagatgt tgtagaggat ctccataacg atctcctgaa
    66001 gaagaggtaa ccgggtattc cacacacccg gaatgttgtg cactcactct aattttcaaa
    66061 agtaatcaca acaagattat ttatgattaa atgtgacgcg acccgcaaat aattccacat
    66121 ggatagaatg ggattcacac aacgaaacaa ctattcattt aaattcattt agttaattga
    66181 attacacatt ccattaaatc tttccacact caaaatcaac aaccacttcc ctggcaaaaa
    66241 gtcaaaaaat ggtcattgcc ttcctgaaaa aatagtgtca ggattaatca aaaccaatca
    66301 catattgatt cgaattgaac tatcatgaag gtaaatggcg atgagtaaag tgaaaagtat
    66361 cacccgagaa tcctggatcc tgagcacttt cccggagtgg ggtagctggc tgaatgaaga
    66421 aattgaacaa gagcaggtcg cccctggcac atttgcgatg tggtggctag gctgcaccgg
    66481 gatctggttg aaatcagaag gtggcaccaa cgtttgcgtt gatttctggt gcggcactgg
    66541 caaacaaagt cacggtaacc cgttaatgaa acagggtcac cagatgcagc gcatggctgg
    66601 cgtgaaaaaa ctgcagccaa acctgcgcac caccccgttt gttcttgatc cgtttgcgat
    66661 tcgccagatc gacgcggtac tggcgactca cgatcacaac gatcatatcg acgttaacgt
    66721 tgctgctgcc gtgatgcaga actgtgctga tgacgtaccg ttcattggac cgaaaacctg
    66781 tgtggatttg tggattggct ggggcgtacc gaaagagcgt tgcatcgtgg tcaaaccggg
    66841 cgatgtagta aaagtgaaag acattgaaat tcatgcgctt gacgctttcg accgtactgc
    66901 actgatcacc ctgcctgccg atcaaaaagc ggctggcgta ctgccagatg gcatggacga
    66961 tcgcgcagta aactacctgt tcaaaacccc tggcggttcc ctatatcaca gtggcgattc
    67021 ccactactct aactattatg cgaagcacgg taacgaacat cagatcgatg tggcgttagg
    67081 atcgtatggt gagaacccgc gcggtattac cgacaaaatg accagcgccg atatgctgcg
    67141 tatgggtgaa gccctgaatg cgaaagtggt tatcccgttc caccacgaca tctggtcaaa
    67201 cttccaggcc gatccgcaag agatccgcgt gctgtgggag atgaaaaaag atcgtctgaa
    67261 gtatggcttc aagccgttta tctggcaggt tggcggcaaa tttacctggc cgctggataa
    67321 agacaacttc gagtaccact atccgcgcgg tttcgatgat tgcttcacta ttgaaccgga
    67381 tctgccgttc aagtcattcc tgtaatctgt cgtttatacc ggatagcgtg gagaagttcg
    67441 cgctatccgg cgtagtattt cctggctatt tcaaatatca tctaaaaaaa tcaaatttta
    67501 tcggaatagc tcatgactga agcacaaaga catcaaatcc tcctggaaat gctcgcacaa
    67561 ttgggctttg tgaccgttga gaaagtcgtt gagcgtctgg gaatttcgcc tgccactgcg
    67621 cgacgcgata tcaataaact tgacgaaagc ggcaaactga aaaaagtgcg caatggcgca
    67681 gaagctatta cccaacagcg cccgcgctgg acgccgatga atctgcatca ggcgcagaat
    67741 cacgatgaaa aagtacgtat cgctaaagcg gcctcgcagc tggttaatcc gggcgaaagc
    67801 gtagtcatca actgcggctc caccgcgttt ctgcttgggc gggaaatgtg tggcaagcca
    67861 gtgcaaatca tcactaatta tctaccgctg gcaaattacc tgatcgatca agaacatgac
    67921 agcgtgatca ttatgggcgg acagtacaac aaaagtcagt ccatcacttt aagcccgcag
    67981 ggcagcgaaa acagtctcta tgccgggcac tggatgttta ccagcggcaa aggactgaca
    68041 gcagaagggt tgtataaaac cgatatgctg acggcaatgg cagagcagaa gatgctgagc
    68101 gtggtaggga aactggtggt actggttgat agcagtaaga ttggcgaacg cgcgggaatg
    68161 ctttttagcc gtgccgatca aattgatatg ctgatcaccg gcaaaaatgc taacccggaa
    68221 atcctgcaac aactggaagc gcaaggggtc agcattctgc gtgtttaaag atgctggcgg
    68281 aaaaatgtca ctgcagcatc taacgcctca ggcgtaatgc ggtgacgcac gcctggctgc
    68341 catgaacagg ttagcagttt atcccgtccc gtctcgctta aggcctgctg caaacgtagt
    68401 gattcgtcgg caggcacaac gtcatcgtcg aggccatgcc acagcagtag aggtctgtca
    68461 ctaagttgtt ccaggtggtt tgtcgcttcc cactctgcca gcggcgcgac aatgttattg
    68521 aattcattct gctgtgctgt cgtttcaggt atcagcggtg gaaacagtga acgggcgagt
    68581 gatgtaaaat agcccgctcc catcatgctg gcggtacatc tcaccgtggg gtggcgagcg
    68641 gtaatcccca gtgccgtcat cgcgcccatc gacgcaccac cgactgccag acggtcatca
    68701 agcagccagt tttcttcggc tattgccgca cgtaaagtag tgaattcctg catactttgt
    68761 agcaagattt gccagaattg atttaaccgc cgcgctgcgt caccactaaa acggctaccg
    68821 tgatcgggcg catccggcat gatcacccgc aaaccagcct gcgccagcgc aacggcaaaa
    68881 tagctataca ccagactgga tgaagtaaag ccgtgataaa aaattacgca cggtaacggg
    68941 gtatcttttt gcccgacagg ataagcatga agaacgggaa tatctgccag ctcgcgtgat
    69001 tctatttcaa tcatctgtct ctcctttttg tctggtatga catgaagaaa tccttagcac
    69061 aagtttctgc tggcggtatt agttcataat gttgagatgt gggttacgct ttcgtcacgt
    69121 tttcattcga aaatcgcgta ccaggtaaca attcgggaac attcccccaa agttaaatta
    69181 ataaggcact acactatgtt tagcaggaaa cgtaatagcg ttatttaccg gtttgccagt
    69241 ttattattgg tgttgatgtt aagtgcctgt agcgcactgc aaggtacgcc acagccagca
    69301 ccgccagtaa cggaccatcc gcaagagatt cgccgcgacc agacgcaagg gttacagcga
    69361 ataggcagcg taagcacgat ggttcggggt tctccggatg acgcattagc agaaatcaga
    69421 gcgaaagctg tcgctgcaaa agctgattat tacgttgtcg tcatggtgga cgaaaccatc
    69481 gtgacaggac agtggtattc acaagccatt ttgtaccgta aataaatcag acaacttaca
    69541 tagtcagctt tacattgctt tgcgtcccta tgcgtttacc tgtgcacaat aaattaccgg
    69601 ctggaagcct gggcgtttat taccgcgatg gaatgccctg caacatgtgg ggaaacgaaa
    69661 aatggagctg acgatgaaac aattacttgc ctcaccctcg ctgcaattag tgacttatcc
    69721 tgcgagcgcc acggcacagt ctgcagaatt cgccagtgct gactgcgtga cgggcctgaa
    69781 tgaaataggt cagatctcag ttagcaatat ttcaggggat ccgcaggacg tggaacgtat
    69841 cgtggcgtta aaggccgatg aacaaggtgc gtcatggtac cgcattatta ccatgtacga
    69901 agaccagcag cctgataact ggcgcgtaca ggcgatactg tatgcatgag tgtcatctac
    69961 agcaaatgaa caatgccgga tgcggcgcga gcgccttgtc cggcctacaa ttttcacata
    70021 tttcatttag ttaatcgaaa ccagcgtcgc atcagtcgta tacgcttaca cacacactcc
    70081 ccccgtcgcc cgcagcaata aatcattctg gatctgctct gacaaccgta cgccgccgcg
    70141 cgtatctaac atcacctgac accacgcctg cgccattggc ggagaagcat atttcaacat
    70201 ttgcgcaccg cagcccagca ggaatagctg atgagtaatc tctcgcctca gttcttcagc
    70261 tggcttacgc agctgctgct gtaaacgacg aaccgcgcga tcaaaatagc gatcctgccc
    70321 tttcacttcc acaaacgctt ccgacaataa gtcgtatacg cccgcttgct tattgagaac
    70381 gcgcagcaca tccaggcaca taatattgcc ggaaccttcc caaatactgt ttaccggcat
    70441 ctcccggtaa agccgcggca gctcgctctc ctcgcaataa ccaatgccgc ccagcacctc
    70501 catcgcttcg gccacaaacg gcatacctct tttgcagatc acaaattttg ccgcaggcgt
    70561 aaataaacgc gcccacaggg cttctttggc atcggcacgc cggtcccacg ctcgcgcaag
    70621 acgaaacagc aacgccgttt gcccttcaag ctgaagtgcc atgcgactta agacatgacg
    70681 cataaggggc tgttggatca atggattacc aaaaacatgg cgttgatgtg catgataaat
    70741 cgccagcgaa aatgcacggc gcatcatggc atggctaccc agggcgcaat caaaacgcgt
    70801 catcccaccc attttcagga tcagacgaat tccttcccct tcctgcccca acaaccagcc
    70861 aatggcatcc tgaaactcca cttcgcagct ggcgttagag cgattaccca gcttatcttt
    70921 cagccgctcg aggcgaatcg cgttgcgttg cccgtcaggc aaaaagcgcg gcacaaaaaa
    70981 gcaggacaaa ccgcccgtag tctgcgccag caccagatgc gcatcgcttt gcggcaccga
    71041 gaaaaaccat ttatgcccca ccagccgata agagccatct tccaggcgct ctgcgcgggt
    71101 ggtgttgctc atgacatcgg aaccgccctg cttttccgtc attcccatgc caatcaacaa
    71161 accgcgtttt tgcccacctg gcaataagtg agaatcgtag cgatcgctca gcagcggcgt
    71221 ggtccagtcc tgaaacggcg cgggtaacat ctgtaacagc aatggcgtgg cggcaaaggt
    71281 catggttatc ggacataacg accctgcctc aacctgtgcg tgcaacataa aacgcgccgc
    71341 gcgcgccaca aatgcgccgg agcgagcgtc ttcttcccag gcaagattgt gcacccgatt
    71401 ggtacatagc gcctgcatca gcaggtgcca ggcggggtga aaacgcacat cgtccagacg
    71461 gcgtccttgc gcatcgtagc gcaataattc aggcggattc acattcgcca gccgccccag
    71521 ttcaagggat tcagccgttc ctaactgctg accaatactg gctagaaaat cgctatccca
    71581 gccagcacct tcacgcgtta ccgcttcgca gagcgcgcca tcagacaggt ataagttgct
    71641 gttatttaat ggtataggtt gattaaaaac ggtgtgagtt tgccagtgca ctgtgtctcc
    71701 ctccatcaat ggcagtcacc attagtatgg tcactgccat ggattcatga cagaaagaga
    71761 ttgctaaaac attcttatcc agcaatgtaa tgcggaacga aacgtgaggt gtctttggtg
    71821 atcagtgtgt tatcttcacg gatccccatt ccgcacgctt catcatcgac aatccagcta
    71881 ccgatgagtg tgtagctatc gccaaatcgc ggcagaggtt gaaacgcctg gtagatcatc
    71941 ggttcatcgg cgtaatcacc atcagcgtgg tcaacgacgt tattctgacc gtcgaaaatg
    72001 gtgacgtttc cgccttcgcg cgagtagatc ggtttacgca catagctttc gccagcggca
    72061 atctgcgatt tttcaccctc aaaccaggat gcaagaaggt taggatgacc agggaagaag
    72121 cgccaaagca gcggcattag ccctttatta ctcaagatac ttttccataa cggctccacc
    72181 cattgctcgc gacgcttgca cagcagcgga ccgttatcgt cacgcatcat ccactccagc
    72241 ggatacagct taaatgcacg ctggatgaca ttatcatcaa gatcggtcag tacaccgccg
    72301 acgcccaaac cgagatcttc aatgtagata aaccgcgact cctgccctgc ctgctgggcg
    72361 cagtcctgca agtacagcac ggtagtcctg tcttcatcgg tgtcctgaca gcagcagaaa
    72421 taaaacggct cccggctata aagctcgcta aagcgtgaaa tcaggcgttc ctgaatggca
    72481 ttgtactgat cggcatcacg cggaataatg ccgctgcgcc gggcatcctc cagccacaac
    72541 cactggaaat aagccgactc atacaacgag gtcggcgtat cggcgttgta ctccagcagc
    72601 ttcaccggcg cattgccaca ccaggcaaaa tccatccggc catacagcga aggatcgcgt
    72661 gcgcgccagc tttcggcgat cacatcccag tacaacggcg ggatcgccag ttgcgtcagg
    72721 atctcttcgt ctttaaccgc gcgatccacc acctcaaggc acatctgatg cagttccgca
    72781 gtcggttttt cgatctgctc ttcaatctgg cgcagagtaa aacggtaagc ccgactctcg
    72841 tcccaataga tttcattgtc gatgatatga aagtcgaaac cgttgttagc ggcgatctgg
    72901 tccagatccc gtcgcacagg aacgttgtgt ctcagcatga ttagcctccc caatgcccac
    72961 gggcgctgga agaacgacca tagccgccgc gcgaaacggt tgacgctttt ttggtggtaa
    73021 agccgcctga agagtaagac tcttttttgc cagaaccgga gcgccaggag taatcaccgg
    73081 aagtgttgcg ccagacgggg cgggaagcaa atgaggatcc gccgctgtta taaacaaact
    73141 gctcatcgcg atctttacga ataacccggc ttaacaaaaa tccagatatt accgggatcc
    73201 aactctgttc aacgttgtcg tagtaacaat tttcatactt agactgacag ttctgctgag
    73261 tcatattctt cggaacatcg gcataaaacg ccgctttggc gttattccag ccacgcgcgc
    73321 aaatgtcggc attattaccg tcatcaatac aatcctgcac tgtcgagtaa aacgttccgt
    73381 cgccgtcgtt atcgacatcg ctactttcac tccatccttt gactattaaa aaaacggcac
    73441 cgcccattag caccaacgtt aagtattttt gtgcgtagcg attgcggcgc ggctcaaaag
    73501 gattatgcgg tcttccaatg cgactgatcg ctccgtgacc aattttactg ttatttcttg
    73561 atttgcgttt tctggccatg atcgtccatt accatgtcat acaagctgcg ttaaaaatac
    73621 cgccagccag cgcggcggtt cccataaaca ttcctgctgc ggtattgtga ttaataattt
    73681 tttcgcttaa tgcgggcata taaagtctga caccagcaaa aacgagtaac tgaatcacca
    73741 gtgcaatccc gccccaggcg aaatagtctg gaatactcac cgcattgatt gccgcactgg
    73801 ataatgggat aacgtaacct aacaatgtac cactgaacgc cagtgaagcc gcggtattat
    73861 tgtttttgat taactgccat tcgttgtgcg gtgtaatttt agaatagata aaaagaaaaa
    73921 taatcaccat agccacgcca ataaaaaaat aggcgctaaa ggcaagaagt gaatccagta
    73981 tatgcattgt aaacgtcctt gtaattaacc aataatatga agtgatgtca gcgggatatc
    74041 gacacccaac gcgcgcgaaa ataaccactc tggctcgccg agatcgttaa aagattcctc
    74101 accatttaac agcaaatatt cgtaagtatc ttcggttact tggcgttggt agcccatggt
    74161 aaaattatgg acctcccatt tggcatggtt ttgattttct actttttcca gcatgtagac
    74221 gggttcgata tttcccggtt cttcgctgtt aaaaaatcgc tgccagcgtt tttcctgcca
    74281 gtttaagatc attgccccca tcactttggc gttgatcgcc tcgcgccagt gactctcttt
    74341 actgataccg tagctctctt catagacaaa gagcttgata tcatcaatgt catcaatatc
    74401 ttcgccgccg gtggtattga tttgcagaaa ttcatcgcct gaagtgtagt agcggaaaat
    74461 ctgactaccg ccgcccagat cgatgtggct gacagcggct accgtaaatt cctcacccgg
    74521 cagcgcaatc agcaattcat cttccagcaa acgaaacgct aacgtgtcga gcgtaaagcc
    74581 actattgaga tgaagtccca gcggaccgcg agcgatagca ggcttattat ccttgccaaa
    74641 cagacgctgg aaaaaaccag acatatttta tccttaaaat agttgccagc cttttagcga
    74701 ggctggcaaa aaaattactc gccctgttgg cgttgcagtc ttgctaatac atcctgggcg
    74761 ctacttttat tgctaccgcc aattccggct tccgccagct tttcatcaag atcgcgaccg
    74821 tctgcgactt tttccaactg tgcagcagca tccaggcgag cctgacgttc ggcctgacgc
    74881 gtttgcaggc gttttaagga ttctgccgct gtcgaaacgc tggaagatgc gccaacggta
    74941 gaggttgtta cagcctgttg tgcacgctgc atggcttcag tggctttaac gacttccatt
    75001 tgctgctcaa actgagcgat acgctgcgct gtcgctgtaa ctgctttttc cacgccatca
    75061 cgggagactt ccagattcga caacacttgc tcttcagcgg taatgagatt ctcaaggcgc
    75121 gcgatttctt cagcaacttc gttaatcaac gacggattaa cgttcttgct taacgcttcc
    75181 agcgcacggg cttccagact ggctttgcgc tcacgtaaat ctttcagctt atcgtgactt
    75241 aatttcaccc gcgccagcag atcaacgcga gattttccgg ctttatcgag ttcagcttta
    75301 gcgtcacgaa tatgctgttc cagcatgcgc acgccctggg tttcttcaat ggattcttcc
    75361 gcctgggaga taaacgattt ccccagcgta aataaacttt ttaaaattcc cataaatacc
    75421 cgtccttgta attagtgtga atattcttct gtaatttcag ccagatccaa tgcgttatct
    75481 accagcgagg ttatctcaag caggatatct tcaagagaag atttaagcga taacgccccg
    75541 aaaacaatgt aatactcttc ctgttgcacg ctggagattc ctaccgatga caacggcatc
    75601 atcttctgat ttcttaataa gaaggtatta aattcatctg gattgctgat actacttacc
    75661 ggacaaataa aggtttcgat aatcatttgg cgggaagtaa aaagaatatt gatttgcaaa
    75721 tcgccataat cattcatttt aataattaat gcgctctcgc tatttgtcac atcaatattt
    75781 tgttcaggta cagtttgcag cgccgtcgcc agcgccaacg gattccatgt catatattat
    75841 tccatataga tttagtttaa atattaataa aatgaatatt tgcagtacgt aattatctta
    75901 ccagctatag acaaaaaaaa accatccaaa tctggatggc ttttcataat tcagacgaac
    75961 tagctgcgct gacgaaccgc ttcaaataag caaattccgg ttgcaaccga aacgttcagg
    76021 gaagaaacac ttcctgccat tgggatgctg atcaactcat cgcaatgttc acgggtcagg
    76081 cgacgcatac cttcaccttc cgcgcccatc accagcgcca ggcgtccggt cattttgctc
    76141 tgatagagtg tatgatccgc ctcgcctgcc gtaccgacga tccagatatt ctcttcctgc
    76201 aacatacgca tggtgcgcgc aaggttagtc acccgaatca gtggaacgct ttctgccgcg
    76261 ccgcaggcta cttttttcgc cgtagcgttg agctgtgcgg agcgatcttt cggcacaatc
    76321 accgcgtgaa cgccagcagc gtccgcgcta cgcaggcacg cgccgaggtt gtgcggatcg
    76381 gttacaccgt cgaggatcag caggaacggt tgatcgagcg aagcgatcag atccggcaga
    76441 tcgttttcct gatactgacg tcctggcttc acgcgggcga taatgccctg atgcacggca
    76501 ccgtcgcttt tctcgtcgag atattggcgg tttgccaact ggataaccac gccctgagac
    76561 tcaagggcgt gaatcagcgg taacagacgt ttatcttcac ggccttttaa aataaagact
    76621 tcctgaaaac gttcaggggc gcgctccagc agggcctgca ctgcgtggat gccgtaaatc
    76681 atttcgctca ttaatgtact cgttgtttac gttttgcctg atgcactacg tttatccggc
    76741 ctacatgatc tctgcaatat attgaatttg cgtgattatg taggccggat aaggcgttca
    76801 cgccgcatcc ggcatgaaca aagcgcattt tgtcagcaat ctaaccctct tcttttaaag
    76861 agggtattga tcactctgcc acttttttct tcgccgcacg cttcgctttg gtcgctgcgg
    76921 ctattttctg cgttttcgcc gatggctttt tcgcttttct cgcgtctttc ttcgccgctt
    76981 tcggcttcgt ttttttctca ccgcggaagg cgctgtctgg ctcaaagttt acctttttac
    77041 cgacctgacg acgcttgccg ccttttttgc ctgcatcgcc ttttttcgct ttctcgcgcg
    77101 ccgttttacc gacgttgcgc ggtgcgcgtt cactggagat cagactaaag tcgattttgc
    77161 gctcgtccat attaaccgct tcgactcgaa cttccacgcg atcgcccagg cgataagtct
    77221 ggccgctgga ttcccccatc aggcgttgcc ctacctggtc aaagcgatag tagtcattgt
    77281 ccagcgaaga gacatggacc agaccatcaa tgaacaagtc gtccagacgg acgaagaagc
    77341 caaagccagt gacgctggaa attacgcctt taaagacgtt acctacctgg tcgagcatga
    77401 agtcacactt cagccagtca gcgacatcgc gcgttgcttc gtcggcacga cgttccgcca
    77461 tcgaacagtg ctgacccagt tgcaacatct cttccatcga ataatggtag ccgccggttt
    77521 cagtggtgtt gccctgatgc ccctgctctt tcgccagcag atatttaatg gcgcggtgca
    77581 gcgtcaggtc aggataacga cgaatcggcg aagtaaagtg cgcataggac tgcaatgcca
    77641 gaccgaagtg accacggttt tctggatcgt aaatcgcctg tttcatcgag cgtagcagca
    77701 tggtttgcag catttctgca tcaggacggt cggcaaccga ctccagcagt tccgcgtagt
    77761 cacgcggttc cggcttgtta ccacccggca gctccagccc cagctccgcc agcactgaac
    77821 ggaaagaggt aatcgcttcg gtgctcggct tgtcgtgaat acggaacagt gccggttctt
    77881 tcgctttctc aacgaaacgc gccgccgaga tattcgccag aatcatgcac tcttcaatta
    77941 atttgtgcgc gtcgttacgc tgggtctgtt cgatacgttc aatacggcgt tcagcgttga
    78001 aaatgaactt cgcttcttcg ctctcaaatg agatcccacc gcgttcttca cgggctttat
    78061 ccagcacttt atagaggtta tgcaactctt cgagatgctt aaccagcggg gcgtactgct
    78121 cgcgcagatc ctgatcgccc tgcagaatat gccagacttt ggtgtaggtc agacgcgcgt
    78181 gagagctcat caccgcttcg tagaatttgt agcccgtcag gcggcctttc gacgaaaccg
    78241 tcatctcgca caccatacac aggcggtcta cctgcgggtt gagcgaacac aggccgttag
    78301 agagcacttc cggcagcatc gggataacct gcgaagggaa gtacaccgac gtgccacggt
    78361 tacgcgcttc tctgtccagc ggcgttggcg gacgcacata gtagctgacg tcggcaatcg
    78421 cgacccataa acgccagccg ccgccgcgtt ttttctcgca gtaaactgca tcgtcaaagt
    78481 cacgggcgtc ttcgccatca atggtgacca gcggtaaatc gcgcagatca acacggcccg
    78541 cttttgcttc ttccggcact tcttctttca gcccggcaac ctgttgctca acagcctgcg
    78601 gccagatgta cggaatttca tgggtacgca gagcgatatc aaccgccatg ccggtgccca
    78661 tattgtcgcc cagcacttcg acgattttac ccaccgcttt ggtgcggcga gtcggacgct
    78721 gagtcagttc gactacgacc acaaagccca tccgcgcgcc catgatctga tcgggcggga
    78781 ttaagatatc gaagctcaga cggctatcgt caggaaccac aaagccgacg cccgcttcag
    78841 taaagtagcg accaacaatc tggctggttt ttggcaccag tacgcggaca atacgcgctt
    78901 cacgacgacc tttacggtca gcgcccagcg gctgcgccag cacctgatcg ccatgaatgc
    78961 aggttttcat ctgctcgctg gagagataca aatcatcttt acgcccttca acccgcagaa
    79021 agccgtagcc atcacggtgg ccaataacgg tacctttcac caggtcgagg cgttccggca
    79081 gcgcatagca ctgacgacga gtgaagacca gttgaccatc gcgctccatc gcgcgcaggc
    79141 ggcgacgcag gccttcaagc tgctcttcgc cttcaatgtg cagttctacc gccagctcat
    79201 cacggctggc cggtttttca cgtttggtta aatgttcgag gataaattcc cgactaggga
    79261 tgggattcgc gtatttttca gcttcgcgtt cctggaaagg atcttgtgac atctcggttc
    79321 ctccgttgtc atctctgatg aagattttcg tcactccacc agcaataatt tataaagcgg
    79381 ttgattctct tcaaccaaat cggcaagcgt gtagttatcc agttccgtaa gaaaactttg
    79441 cacggcctta gaaagcgcct gtttcaaccg acaggcaggt gtaatgtggc aaaactcact
    79501 gctgcaattc accagcgata agggctccag ctcgcgcacc acatcaccaa tacgtatcgc
    79561 actcgccgat ttacccaggc gaatgccgcc attttttcca cgaacagcag tcacgtagcc
    79621 ggcacgacta agttgattga ttattttgac catatgatta cgggagacgc cgtagacgtc
    79681 agtcacttca gaaatactgg tcatccgccc ttctggcaat gacgccatgt agatcagcgc
    79741 acgtaatccg taatcagtga aactcgttaa ctgcacatca acctcaaaag ggaaatcggg
    79801 aaaaaataca tttatattga tgataaacca gccacaagct gtgtcgctaa tttatttcag
    79861 ttcggggaag gaaaaaagcg aggatttaac actgtgtcgg atagcgtaat ggcttaaccg
    79921 acacagcaat gtatcacact gttagcccgg caggcaaaat atctgccagg cgtaccagaa
    79981 ttacgcgtcg aacggatcgc gcagaatcat ggtttcagta cgatccggac cggtagagat
    80041 gatatcgatc ggcacaccag tcagctcttc aatacgcttg atgtagttca gcgccgcctg
    80101 cggcagaccg ctacgatctt tcacgccgaa ggtggattca gaccagcccg gcatggtttc
    80161 gtaaatcggc tctacacctt tccagtcgtc agctgccagc ggagtggtag tcacttcgcg
    80221 accatccggc atacggtaag ccacgcagag tttaacctct ttcaggccat ccagaacgtc
    80281 cagtttagtc aggcagaagc cagacaggga gttcagctgt accgcacgac gaacggcaac
    80341 ggtgtccagc cagccggtac gacgacgacg ccccgtagtt gcgccgaatt cgttaccctg
    80401 cttgcagagg aactcgccag tttcatcaaa cagttcggtc gggaacggac ctgcacctac
    80461 acgagttgag taagctttga ggatacccag aacataatca acataacgcg ggcccaggcc
    80521 ggaaccggtc gccacgccac cagcagtggt gttggaagaa gttacgtacg gataagtacc
    80581 gtggtcgata tccagcagcg taccctgcgc accttcgaac atgacgaaat cgccacgctg
    80641 acgcgcctgg tcgagcaggt cagaaacgtc aaccaccata gaagtcagga tgtcggcaac
    80701 agccatcgta tcatccagaa ctttctggta atcaaccgct tcagctttgt agtagttaac
    80761 caactggaag ttgtgatatt ccatcacttc tttcagtttt tcagcgaagg tttctttgtc
    80821 gaaaaggtcg ccaacacgca gaccgcgacg tgctacttta tcttcataag caggcccgat
    80881 accacgaccg gtggtgccga tcgctttcgc gccacgcgct ttctcacgcg cgttatccag
    80941 cgcaacgtga taatcaagga tcagcggaca tgcttcagac agcagcagac gctcacgaac
    81001 ggggatgcca cggtcttcca gttctttcat ctctttcatc agcgcggccg gagacagcac
    81061 aacaccgtta ccgatgatgc tggttacatt ctcgcggaga atacctgatg gaataagatg
    81121 gagaacggtt ttttcaccgt tgattacgag agtatggcct gcgttgtgac cgccctggta
    81181 gcgtacaaca tatttagccc gttcagtcag aagatcgacg atcttacctt taccttcgtc
    81241 accccattgg gtgcccagta cgacgacgtt gttacccatt tttcaaaatc accgtttgct
    81301 taaaaatgga ttctaccatc gctttttcag agttacagca ctttttgcac tcaaaaatga
    81361 cctcgatcag tctgtttttt gttcagccaa tcgttttcct caacatgtag tagaccacaa
    81421 cgcccgcaac cacaagtcca ccgccaaaac gacgtaaaat attatcgggc aaattggtca
    81481 tcgcagagat catcttcttc catgccttcg ggtaaagcat cggccctaaa ccttccagta
    81541 ccaaaaccag ggcaagcgcc agccagattg tcgaattcat tcattatcct tatagaaaaa
    81601 gaaaaccacc gacatccctg aggatgcggt ggctttattg acctgtaccg cagtcgttat
    81661 attaacgcgt tgcggaagtc ggcgtcttca tgtagcggaa gaaatcgcta tccgggctca
    81721 tgaccatcac gtcctgattg ccagagaagc tgttctcata agcacgcagg ctacggatga
    81781 atgcgtagaa gtccggatct ttactgaatg catcagcaaa cagtttggct gcttcggcat
    81841 cgccttcacc acgcatgatg cggccctgac gctcagcttc tgccagcgtt ctggtcactt
    81901 catagtcggc agtcgcgcgc agtttttccg cttcttcctg accttgtgaa cggtgacgac
    81961 gcgctaccgc ttcacgctcg gcgcgcatac ggttgtagat cgcttcagac acttcggtcg
    82021 gcaggttgat ctgcttgata cgcacatcga caacttcaat acccagcgcc gccatactgt
    82081 tcgggttgat gaccggaact ttgcccttcg tctcagccgt tacgcgctct gccgcttcgg
    82141 caatggcgtt atctgctgcc ggggtagtaa cttcatcttc tgtacccgca gaaccggagt
    82201 tcagcgcgtc acgtacttcg agggtcagac gaccacggga atcggtgacg atgtctttca
    82261 cgtccaggcg accaatttca gaacgcagac ggtcagagaa cttacgtttc aacagcactt
    82321 ccgcttgcga aatgtcgcca ccacccgttg ccaggtagta acggctgaaa tcgctgatgc
    82381 gccatttgat gtaagagtcg acgatcaggt ctttcttctc tttggtcaca aagcggtcgg
    82441 cctggttgtc catggtctga atacgtgcgt cgagcatttt caccgtttca atgaacggta
    82501 tcttgaaatg cagacccggc tcataaacca gaggtttgtt gtcatcgtca cgcagtacct
    82561 taccaaaacg cagcgtaata ccgcgctcac cttctttgac gacaaagaca gacatgtaaa
    82621 gcactaccag cacgatgatg ataatcgcga taactgactt acgcatcgtt attccccctg
    82681 acgctggtag tcgttacgct gcgcgttggc gcggcgttgg tccataatat cgccctgact
    82741 ggtggacgac gtgttgcttg ctccactggt tgtggaggaa gaggctggcg gcaaacgcag
    82801 cagattgctg gcaccgttat cgctcttcgc cgcaggggcg ttaccacctt tcagcatctg
    82861 gtctaacggc agaaccatca ggttgccacc tttatcgtta accagcactt tgcgggtgtt
    82921 acccaacact ttttccatcg tctcgatata cagacgctcg cgagtaattt ccggcgcggc
    82981 tttatattcc ggcagaagtt tagcaaagcg cgccacttca ccctgagctt ccaggatggt
    83041 ctgggccttg tacgcacgcg cctcttcgag gatacgttgc gcctgaccgt tcgcacgcgg
    83101 ctgaacttcg ttggtatacg cttctgcttc acgaatgtat tgctgttcgt tttcacgcgc
    83161 ggcaatcgca tcgtcaaacg ccgcttttac ttcttccggc ggacgagcag cctggaagtt
    83221 gacgtccagc agagtgatgc ccatgtcata cggacgaatc gtctcttcca gttcgcgctg
    83281 ggtatcgcta cgaatcacgg tacgaccttc cgtcagaatg cggtccatgg tgtatttacc
    83341 gataaccccg cgcagggcgc tgtcggtagc ctgacgcagg ctgtcatccg ggctggtcac
    83401 gctatacaga tatttttccg gattggtaac gcggtactgc acgttcatct caacgcgcac
    83461 tacgttctcg tccgacgtca gcatcacacc ggaagcggcc agttcacgca cggcttccac
    83521 gtttaccggt ttgacttcgt cgataaacgt cggtttccag ttcagacccg gctcaaccag
    83581 atggctgaat ttaccaaagc gtgttaccac gccgcgttcg gcttctttaa tggtatagaa
    83641 accactggcc gcccagataa tgacaatcgc tgccgctgcg atggtaacga cacgaccgcc
    83701 aagctgcggg cgcgggcctt gcgatgaact gccaccgcca gatccggtgc ctttaccgcc
    83761 gcccagacca ccgagctttt tgctcagttt gcggaagata tcatctaaat caggtggccc
    83821 ttgatcgcga ccgcctttgt ttccatttcc ctcagagttg ccgccaggtt tgctgcttcc
    83881 ccacgggtcg cggtcttgtc cgttattacc gggctgattc cacgccatgt ttgtgctcca
    83941 tatttgttat gcggtgatcc ctgttgtctt tcgacgccag aggatatgac tccacgcttc
    84001 agacgctacg ccgttagatc aggtaatcga tcaacgccgg ttcttgttta cagaggcgac
    84061 gccagtcaac gatcggcata cgaacttgca gacttacgct gccgtcctcc tccatccact
    84121 ctttttctat tgcctgaagc tgataaaaac gacttctcag acgcccttcc tgcggtggca
    84181 gacgcaatgt atgctgcgcc acctcgccgg aaagccgctc cgtcaaagcc tgaaaaagct
    84241 gtggtatccc cgctccggtc tgtgcggaaa gccagacacg gatcggtttg ttctcttcgt
    84301 cccgatcaat acgcggttcg aaatcttcca gcatatcgat cttgttcatc accagcaggg
    84361 ttgggatctc gtgagcgtcg atctcttcaa gaaccgtatt caccgcttcg atgttttctt
    84421 gtacacgcac atccgccgca tcaatgacgt gcagcagtaa tgtggcttgc cgcgtctctt
    84481 gtaacgtggc tttaaatgcc gccaccagat cgtgcggcag gtggcgaata aaccctacgg
    84541 tatctgcaag tacggtttca ccgacatctg caacgtcaat acgccgcaac gtcgggtcga
    84601 gggtggcaaa caactggtct gccgcgtaga cccgcgcttc ggtgatgcga ttgaaaaggg
    84661 tagatttacc ggcgttggta tatcccacca gcgaaacagt aggaacgtcg gctttgatac
    84721 gcgattgccg cccctgctca cgctgctttt caactctttc caggcgtgac tgtatctgca
    84781 cgatgcgatt acgcaacaaa cgacggtcgg tttcgagctg ggtttcaccc ggaccacgca
    84841 aacctatccc gcctttctgt ctttcaaggt gggtccagcc acgcaccagg cgcgtagcca
    84901 gatggcgcag ctgcgccagc tcaacctgca acttaccctc atgggtacgc gcacgttggg
    84961 cgaaaatatc taaaataagg ccggtgcggt cgataacacg acactcgcac aaacgctcca
    85021 ggttacgctc ttgcgccggg ctcagggcat ggtcaaaaag aacgaccgaa gcacccgtcg
    85081 ctttgacagc ttccgcaatt tcaactgctt taccttcacc tacaaaatac tttgggtgcg
    85141 gcgctttacg gctaccggta atcacctgca atgcttcgac accggcggaa gagaccagag
    85201 attcaaactc ctggaggtct tccatatctt tgtcttgcgt aaaatagatg tgtaccagta
    85261 ccgcctgctc accagcatca taacggtcaa acaagcgtat aaccctctaa atagatcagc
    85321 ggggaacgca ggatcgctgg ctccccgtgt aaaaaaaaca gcccgaaacc ttattcggtt
    85381 tcttcgctgt cctgttgcgc ggaagtattc tgcgcgctgc taccatgatg gtagttactg
    85441 ctggtaccgc caccggcgtt gttactgtga tgagaaaccg ggcgagacgg gacaacagta
    85501 gaaatcgcgt gcttgtaaac catctggctg accgtgtttt tcaacaggat cacgaactga
    85561 tcaaaagact cgatttgccc ttgcagctta ataccattca ccaaataaat agaaactgga
    85621 acacgttccc gacgcagtgc gttcaggaac ggatcttgta aagattgccc cttagccatt
    85681 ctctcttttc cttatatgct tatttgtact ttgaaccttt cgattctgaa aaaattgcgc
    85741 acgatacgtc tcaattgtac acattcagcc tgcgatagca ccaacaacct gtaatacttc
    85801 gtcacgcgcc tgttctggtt tttcactgtc aagccagtga accccttccc aaccacgcag
    85861 ccaggttatc tgccgcttcg ccaactgtct cgtggcgcaa acacctcgat aaaccatttc
    85921 atcgtatgag atttcgcctt caaggtaaga ccacatctgg cgataaccca cgcaacgaat
    85981 ggaaggcaag tccgtatgca aatctcctcg ggcaaaaagc gcccggactt ctgcttcaaa
    86041 acctgaagcc aacatctgat gaaaacgctg ctcaatgcgt tgatggagca gttcacggct
    86101 cgccggggcg atggcgaact gatgcacctg atacggtaga gcgtctcctg acgtttgcgt
    86161 cagttccgtt aaagttttac ccgaaatgaa aaaaacttcc agtgcccggg aaagcctttg
    86221 tggatcattt ggatgaatcc ttgctgccgc aaccggatct acctcctgaa gttgacgatg
    86281 caatgactcc caaccttgct ctgccgcctg ttgctcaatt ctggcccgca cttccgggtc
    86341 tgccgacggt agcggcgaca acccttccag caatgccttg aaatacaaca tcgtaccgcc
    86401 cactaacagt gggatccgcc ccgccgcggt gatatcggcc atttccgcca gcgcatcgcg
    86461 gcgaaaatca gcagccgagt aagcctgcga cggatcgcga atatccagca atcggtgcgg
    86521 cgcggcgagt aactcttcag cgttcggctt cgccgtcccg atatccatcc ctttgtaaat
    86581 aagggcagaa tcaacgctta tcaactctac tggtaaaatt ttacgcagct caatggctaa
    86641 cgccgtttta ccggaggccg tcggccccat caaaaaaatc gccttaggca ggctcgcctt
    86701 actgatatca ctcatctttc agggctttta tcgccggatg taaatcaaca gattgtaaca
    86761 gaccacccgg cggcgttttc acaagttgcg gacataaccg ttccacgtcc gccagcaggg
    86821 ttatggcctg tgccattgac cactgcgcat gttcgctcat cagatttcgt gcaatccact
    86881 gcgcaatatt gccaggttcg aatacggact gcttcgccag gtagcctatc agttcaggaa
    86941 tcaagatttg taaattttgt tggcgtaagg gtaaaggcac ggccctgatg gtcacatgct
    87001 gtgcatctga ctggaaatca atacccaatt ccgccagggc agactgcgct ttttctaatg
    87061 ccgatttttc ttcggcagaa acttttagcc gcaacggaat cagcagcggc tgggcgcaaa
    87121 cgggcgcttc acccggcgtc aattgtgcct gacgtagcca acgttctgcc actggcaagg
    87181 ataaaagtga aatgttgcca tcgcgctcca gcaacgcaca gtcggaatgg acgatagtca
    87241 gtacccgacc aaaactctga ctgttcgccg caagtgcagg ttcctgcggt tccggcgctt
    87301 ttaatttttg catcggcgcg ggcgtttgca aaagctggcg atacacttca ccttgctgtt
    87361 tctggtagcc tggctgcgca ttcggccagg gggcagccgg acgactgcct gatgctggcg
    87421 caggagtgta gcgcggagct accggctcac gagctgccgg ttctgcaaag tgattgcgcc
    87481 ccgccgccac gcggttttcc gggatggcac gcggtgcggg ttgtggttca tcgtccagcg
    87541 gtagcggcgt ttccagttgc tgctgtagca cgctcagcac gccctgatag ataaagtcat
    87601 gcaccagacg cgactgatgg aaacgcactt cgtgtttggc ggggtgtacg ttgacgtcca
    87661 cctgatgcgg gtcgatctcc agatacaaca caaacgccgg ttgttgatcg gcccccagtt
    87721 tgtcttcgca ggcctggcgg atcgcatgat tgatcaggcg atcgcgcatc atgcgaccat
    87781 tcacgtagca atactgaatt tccgccagtg cgggcgtagt gtgatttgga tcggccaccc
    87841 agccgcgcag ggtgagatcg ccgtgttgcc attcaatcgc cagcgcttgt tcgagaaaag
    87901 cggtgccgca aatcgcgcct aagcgccgtt ctttttgccc gccttccggc actgcgcggt
    87961 actgacgcac aattttaccg ttatgcgaca ggttgatcgt gacgtcgaaa cgcgccagcg
    88021 caatgcggcg gatgatctca tcaatgtggt taaattcggt tttctcggtg cgcaggaatt
    88081 tgcgccgcgc cggggtgttg tagaacagat ccagcacctc cagcgtcgtc cccacaggat
    88141 gcgccgccgg ttttaccgtc acgttcatat cgcgcccttc ggcataggcc tgccaggctt
    88201 cctgctgttc tgcggtgcgt gaagtgagcg tcaggcggga aaccgaactg atactcgcca
    88261 gcgcctcacc gcgaaagccc aggctgataa tggcttcgag atcgtccaga gaggcgattt
    88321 tactggtggc atgacgcgcc agcgccagcg ccagctcgtc ttttttgata ccgcagccgt
    88381 tatcacgaat gcggataagt ttcgccccac cgcgttcgat atcaatatcg atacgtgtcg
    88441 cacctgcatc gaggctgttt tccaccagtt ctttgactac cgacgcaggt cgctcgacca
    88501 cctcacctgc ggcaatctgg ttcgccagtt gtggcggtaa gacctgaatt ggcatcaatc
    88561 gtccttagtt tggcagcgtg cgatctggcg tcgtcaccgt actggccgtt tgtgccgttg
    88621 ccccctgcgg cgcagattgc atcggatgcg caaggaaata attgcgcagg cctttgtaaa
    88681 tggcttctgc cagctgttgt tggtaatcgt cgctcgccag caaacgttct tcgctgttgt
    88741 tgctgataaa accggtttcg accagtactg atgggatatc cggcgaacgc agaacgccga
    88801 gactcgcgtg ttctggtcgg cgtttatgta tttcgccaat gcgttgcaac tgactgatca
    88861 tactggtcgc tacatcatac cctacccgtt gggaatgacc gaactgtaaa tccagcaccg
    88921 cctggcttaa ataggggtca gactgactgt tcgccagcac atcacccgcc ccacccagca
    88981 gctccgactg tttctcgtgc tgctccagcc agctggccat ttcgctgttg gcgcgacggt
    89041 tagagagcac ccatacggaa gcgccagtcg cactgcggtt cggtgcggca tcagcgtgaa
    89101 tcgacacgag gaaattggcg ttttgcttac gtgccacatc actgcgcccc atcaccgaga
    89161 taaagtaatc cccgtcacgg gttaaaacgc ccttaaacat cggatcgtca ttgagcaaag
    89221 tacgcaattt acgcgcgatg gcgatggtga catttttctc ccgcgtacca ccggggccga
    89281 tagcgccagg gtcctgaccg ccgtgtccgg catcaatagc gatgataatt ttatcgccag
    89341 tgttagccgt cgcgcgcgct gccggacgcg ttaccgtatt actgctgata acacccgtag
    89401 tgcggttact ttccgtttta aacggattgc gcgccggttc gctgacgcgc ggtgcgacaa
    89461 ccgcaggcgt ttcaacgcgt ttcgcaacca cgggcggcgg aggaggaggt ggcggcgcat
    89521 cggcgttaat cgtaaagacg acagtgtaat tgctgccatt ctgccgcttc accgcttcgg
    89581 ttttaccgtt ttcggtaaga tcgaccacca gccgtagcgt ttgtgcatct ttaggcgttc
    89641 cagagcgaat cgccttcacc agattattgc cgctgaacaa caacggcagt ccctgaatca
    89701 cgcccgtttg tttgatatcg agcgccacgg tgcgtttgct ttgatggcta aacgcataat
    89761 caggatcgcc aataaaactc aacgttatcc gcgcctgttg attaccgtta gaaacctgaa
    89821 tatcagagag cgtcgcggca cccaccggcg tgcacagcag cagcagcgtc gctaccaacc
    89881 aatttctgat gcgatacatc atcccgccac ctttcaaagg ttaaccggct aaacgcgcca
    89941 gcaacaattc acccgcagag gaaaccgcac tcacgcgcgc ctcacggcct tgtgcctgat
    90001 aatcaatgtg tatttcgaca tccgggtcag gaagaacacc tgtaccttgt tgtggccact
    90061 ccaccaggca gatggcatcg ttggcaaaat aatcgcggat ccccataaac tccagctcct
    90121 cgggatcggc aaggcggtac aaatcaaagt gataaaccat taaattgtcg agcgtatagg
    90181 gttcgaccag cgtataagtg gggcttttga cattaccctg atgacccaga gcttgtaaaa
    90241 agccccggct aaaggtggtt ttacctgcgc ctaaatcgcc atacagatag attacggttg
    90301 cgccatcgca ggctttcgct acccgctcgc ccaggtctaa tgttgcctgc tcatcaggga
    90361 gcggaattac tcgattcatc atggttttta tcagtcactt ccgggttaac aatacgctgt
    90421 agcgtggaaa agagatcggt tgccagcatc ccgcgcgttc caaaacgcgc cgccagtacg
    90481 tcagctgcag caccgtgcgc gacacagccc gcacaggcgg catcatacgg cgacagtttt
    90541 tgcccaagca atgcgccaat aataccagag agcacatcgc ccatgccgcc gctcgccatg
    90601 cctgcatttc cggcatcaat aatgcctaaa gcgtcaggat gggcggcgac cacggttccg
    90661 gcacctttca gcaccgctac gccgccataa cgttgtacca gacgtttggc gcaatgtaag
    90721 cggtcacttt caatttcagc gacggaacag cctaacaacc gtgcggcctc gccaggatgc
    90781 ggcgtgatca cgcgattgtg acgcttatcg ggattgattg ccagcaggtt caatgcatcg
    90841 gcatcccaca acatcggttt gcgaaaattc tcaacttttt gcagtgcttt tttcccccac
    90901 tcttgctggc ccagaccggg accaatcacc accacatcgg cccattccag gctttcggta
    90961 agagagtcca tcgtcagttc atgcaccatc aattccggtc gtgcagtcag cagcggcgca
    91021 atgttttcac tgcgggtcag tactcggact aaaccagcac cagcacgtag cgccgcttcc
    91081 cccgtcatac gaatagcccc cgccgtgccg tgatcgccac cgataattac cagccgcccg
    91141 tgatcgcctt tatgcgaagt cgggcgacgc ggttttagcc agtgagaaag ttgttctgcc
    91201 gaaaaccgct gaattttcgt ctcctgacct gccagccaac tatccagccc cagtgagtca
    91261 aaatgcagtt gtccggtaac atcccgcgct tttccagtga gcaagcctgg tttcagcgca
    91321 ataaaagtga tggtgtgatc ggcgttgatc actgcgcctg gcgtagcgcc ggtttcagcc
    91381 agcaggccgg aagggatatc aaccgccgca atcggcgcag gatgggaatt agcgtggtcg
    91441 attaactggc taatggattc gcggggcgct tgctgcaagc cggtaccgag cagcgcatca
    91501 acaatcagat ctaccgattc gggccagaca atattcgaag catggatctc gcctcccgcg
    91561 tttaaccatg cttcgcgtgc cagcgcggcc tcttccggca acggtttgtc gctctcctgg
    91621 gccagcagcg tgacctcaat gccgaccgct gtggccagcc gcgcgaccac gtagccatcg
    91681 ccgccgttat taccatgacc gcacagcacc agccaatggc gggcgtcagg atacgccgaa
    91741 cgacacacct ggaatgcggc ttcgccagcg cgaagcatca gctcatagag tgtaagcccc
    91801 agcgcatctg ccgcctcacg ttctccgcgg cggatatcgt cggcgtgcca gacggtgtgt
    91861 ggtatactta cggggttttt cttcattgta tggtccgtca tgtcagagcc cctcgatctc
    91921 aatcagttag cgcaaaaaat taaacagtgg gggctggaac tgggctttca gcaggtaggt
    91981 attaccgata ccgatctcag cgagtccgag cccaaactgc aagcatggct ggacaaacaa
    92041 taccacggcg aaatggactg gatggcacgt cacggtatgc tgcgcgctcg cccccatgag
    92101 ttattgcccg gtacgctgcg cgtgatcagc gtgcggatga attaccttcc tgctaacgcc
    92161 gcatttgcca gcacgctgaa aaaccccaaa ctcggctatg ttagccgtta tgcgctgggc
    92221 cgtgactatc acaaacttct gcgcaaccga ctcaaaaagc tgggcgagat gattcagcaa
    92281 cattgtgttt cgctgaattt tagaccgttt gtcgattctg cgcctattct cgagcgcccg
    92341 ttagctgaaa aagctgggct cggctggaca ggtaagcact cacttatcct caatcgcgag
    92401 gccggttctt tcttcttttt aggcgaattg ctggtcgata ttccgctgcc cgtggatcaa
    92461 ccagtcgagg aaggatgcgg taaatgcgtg gcctgtatga cgatttgccc gaccggtgcc
    92521 atcgtcgagc catataccgt cgatgctcgc cgctgtatct cttatctcac catcgaactt
    92581 gaaggggcga tcccggaaga gttgcgaccg ttaatgggaa accgtattta cggttgcgat
    92641 gactgccagc ttatctgccc gtggaatcgc tattcacaac tcaccacaga agaggatttc
    92701 agcccgcgta agccgctaca cgcaccggaa ctcattgagt tattcgcctg gagcgaagag
    92761 aagtttttaa aagtcacgga aggatcggcg attcgtcgta ttggtcacct gcgttggctg
    92821 cgtaatatcg ccgtagcatt aggcaatgcc ccttgggatg aaacgatttt gacagcgctg
    92881 gaaagtcgta aaggtgagca cccacttctc gatgagcaca tagcgtgggc gattgcgcag
    92941 caaatagaga gacgaaatgc gtgcatagtc gaagtgcaat tgccgaaaaa acagcgtctg
    93001 gttcgggtga ttgaaaaagg gttaccgcgt gacgcctgat tcatccacag cttgtgaata
    93061 aaaataaaaa cgcattgcaa ttcaagacga ataaaatcgt caagtgatcg actcgacaaa
    93121 acggaatctt aatttttaac ttatctatca ataggttaaa aaaatactat tcaccgtgcg
    93181 aagtttcttt gaaattaaga ttacagcctg aggctgtgga taactctgtt tacaagagtt
    93241 tgtcagagac aacgaaaacc agcaataacg catcgcatcg ctgtggatat tttattgaga
    93301 gaagaatttg gagcgggaaa cgagactcga actcgcgacc ccgaccttgg caaggtcgtg
    93361 ctctaccaac tgagctattc ccgcttgggt ggtctgtgcc ttacagcact ttcaaatttt
    93421 ggagcgggaa acgagactcg aactcgcgac cccgaccttg gcaaggtcgt gctctaccaa
    93481 ctgagctatt cccgcgtact acttaatttt gctttcgtaa ttttcgcatt tctgcgtcgt
    93541 tacgggaggc gcattatacg agaaatcttt ccctctgcaa ccccccaaaa aagttttttt
    93601 tgcgttttta tctcaagtgc tgaaattatc agcaagacgt tcaatttagc gtcaaaatca
    93661 cgccgccgca agtggattcc ggcgcttacg tgaccaggaa aaattttaca gcttgataaa
    93721 atgctcgcgg tagtaagcca gctccgccac cgattcacgg atatcatcca tcgcctgatg
    93781 cgtcccctgc ttggtaaaac catccagaat ttccggcttc cagcggcgcg ccagctcttt
    93841 cagggtgctg acatcgagat aacggtagtg gaagtaggct tccagctccg gcatgtattt
    93901 aaacaggaaa cgacggtcct gaccgatgct gttaccgcaa atcggcgatt ttcccgcagg
    93961 cacccactgt tttaaaaatt cgagcgttgc cagttcagct tcccgatcgc ccatcgtgct
    94021 cgctttcacg cgctctacca gcccgctggc ggtatgggtg cgcacgttcc agtcatccat
    94081 cagcgccagc tgttcatcag actggtgtac tgcaatggtc ggcccttctg ccagaatatt
    94141 caggttggca tcggtcacca gcgtggcaat ctcaataatg cgatcgcgct cgggatccag
    94201 accggtcatc tcaagatcga tccaaatcag gttgttttca ttggcactca tgctattttc
    94261 cacccattca cagtgactat attgatccac aattgcgtgt atcatagatg ttttgcccat
    94321 caggggcgac caggagtctg tacgattgag taaaaataaa ctctccaaag gccagcagcg
    94381 ccgcgtgaac gccaatcacc agcgtcgtct taaaacgtct aaggagaagc ccgactacga
    94441 cgacaatctg tttggcgagc ctgatgaagg tatcgtcatc agccgctttg gtatgcacgc
    94501 tgatgtggaa tccgccgatg gcgacgttca ccgctgcaat attcgccgta ccatccgttc
    94561 gctggtaacc ggcgaccgcg tagtctggcg tccgggtaaa ccggcggcgg aaggcgtaaa
    94621 tgtcaaaggg atcgtggaag cggtgcatga gcgtacctcg gtgttgacgc gtccggattt
    94681 ctacgacggc gtaaaaccta ttgccgccaa catcgaccag attgtcattg tctccgccat
    94741 tttgccggag ctgtcgctca atattatcga ccgttacctg gtggcctgcg aaaccttgca
    94801 gattgagccg attattgtgc tcaacaagat agacctgctg gacgacgaag gcatggcgtt
    94861 cgtcaacgag cagatggata tctaccgcaa tatcggttat cgcgtattga tggtttccag
    94921 ccatactcag gatgggctaa aaccgctgga agaggcgttg accgggcgca tcagcatttt
    94981 tgccgggcag tctggcgtcg gcaaatccag cctgctgaat gcgttgctgg ggctgcaaaa
    95041 agagatcctg accaacgatg tctctgacaa ctcggggctc ggtcagcaca ccacgaccgc
    95101 cgctcggctg tatcacttcc cgcacggcgg tgatgtgatt gattcccctg gtgtgcgtga
    95161 gttcggcctc tggcacctgg agccggaaca aatcactcag ggctttgtcg aattccatga
    95221 ctacttaggt ctgtgtaaat atcgcgattg caaacacgat accgatccgg gctgcgctat
    95281 ccgggaagcg gttgaggaag ggaaaatcgc ggaaacccgt ttcgaaaact atcaccgtat
    95341 tctggaaagc atggcgcagg taaaaacgcg taaaaacttt tctgatacgg atgactgaca
    95401 attaagctaa ccgtcgttag aatcgtcccc tttttttcag gatccggcat gtaggccgga
    95461 tcaggaacga caaaacaatg gcctggaggc taccttgtta aattcattta aactttcgct
    95521 acagtacatt ctgccgaaac tatggcttac tcgcctggcg ggttggggcg caagcaagcg
    95581 ggcaggatgg ctgacaaaac tggttatcga tctgttcgtt aaatactaca aggtcgacat
    95641 gaaagaggcg caaaagccgg acaccgccag ctaccgcacc tttaacgaat tctttgtccg
    95701 tccgctgcgt gacgaagtac gcccaatcga taccgatccg aatgtcctgg tcatgcctgc
    95761 cgatggcgtt atcagccagt tgggtaaaat cgaagaagat aaaatcctgc aagccaaagg
    95821 ccacaactac agcctcgaag ccttgctggc aggcaactat ctgatggcgg acctgttccg
    95881 caacggtacg tttgtgacca cttacctctc cccgcgtgac taccaccgcg tacacatgcc
    95941 gtgcaacggt attctgcgtg agatgatcta cgtgccgggc gatctcttct ccgttaacca
    96001 tctcaccgct cagaacgtgc cgaatctgtt tgcccgtaac gaacgcgtga tttgcctttt
    96061 cgataccgaa tttggcccaa tggcgcagat tctggtcgga gcgacgattg ttggcagcat
    96121 tgagacggtc tgggcgggca ccattacgcc gccgcgcgaa ggtatcatca agcgctggac
    96181 ctggcctgcc ggggaaaacg acggttctgt ggcactgctg aaaggccagg aaatgggtcg
    96241 ctttaaactc ggttccaccg ttatcaacct gtttgcaccg ggtaaagtga atctggttga
    96301 gcaactggaa agcctgtctg tcacgaaaat tggccagccg ctggcagtat ctaccgaaac
    96361 ctttgttacg ccagacgctg aacctgcccc gcttcctgct gaagagatcg aagcagaaca
    96421 cgacgccagc ccattggttg acgacaaaaa agaccaggtc taatccatca aaggaaacgc
    96481 tgacgtgcgc ctgattatca cttttctgat ggcctggtgc ctcagttggg gggcgtacgc
    96541 cgcgacggcc cccgatagca aacaaatcac tcaggaactg gagcaggcaa aagcggcgaa
    96601 acccgcacag ccggaagtcg tagaggcgct ccagtctgcc ttaaatgcgc ttgaggaacg
    96661 aaaaggttcc cttgagcgca tcaaacaata tcagcaagtt atcgataatt atccgaaact
    96721 ctccgctact ctgcgcgcac aattaaacaa catgcgtgac gagccgcgca gcgtgtcgcc
    96781 gggaatgtct accgacgcgc tgaatcagga aattctccag gtcagcagcc agttgctgga
    96841 taaaagccgt caggcccagc aagagcagga gcgcgcccgc gagattgccg attcgctgaa
    96901 tcaactgccg caacagcaaa ccgacgcccg ccgtcagtta aatgagatcg agcgccgcct
    96961 ggggacgctt actggcaata ctccgctcaa tcaggcacaa aatttcgcgt tgcagtctga
    97021 ctctgcacgt cttaaggcgc tcgttgatga actggagctg gcgcagctgt ctgccaataa
    97081 ccgccaggaa ttagcgcgct tacgctcaga gctggcggaa aaagagagcc agcaactgga
    97141 tgcgtatttg caggccttgc gtaatcaatt aaacagccaa cgtcagcttg aggcggagcg
    97201 ggcgctggaa agtaccgaat tactggcaga aaacagtgcc gatttgccga aagatatcgt
    97261 cgcgcaattc aaaattaacc gcgaactatc ggcggctttg aatcaacagg cgcagcggat
    97321 ggatctcgtt gcctcgcaac agcgtcaggc tgccagccag acgttacagg tccggcaggc
    97381 gttgaatacg ctgcgtgaac agtcgcaatg gctgggatcg tccaatctgc tcggcgaagc
    97441 gctgcgggcg caggtggcac ggctgccaga aatgccaaaa ccacaacagc ttgataccga
    97501 aatggcgcag ttgcgtgtgc aacggttacg ttatgaggat ctgcttaata aacagccgct
    97561 gctacggcaa atccatcagg ccgacggtca gccgctgacc gccgagcaaa accgtattct
    97621 ggaagcacaa ctgcgcactc agcgtgagtt gctgaactca ttgttgcagg gtggcgacac
    97681 gctactattg gaactgacca agctgaaagt ctccaacggg caactggagg atgcgctgaa
    97741 agaggtgaac gaagcaacgc accgctatct gttctggacc tctgacgtgc gcccgatgac
    97801 catcgcctgg ccgctggaaa tcgcccagga tctgcgtcgt ctcatttcgc tggacacctt
    97861 cagtcagttg ggcaaagcca gtgtgatgat gctgaccagc aaagagacaa ttttgccgct
    97921 gtttggcgcg ttgattctgg tcggttgcag tatttactcg cgccgctatt tcacccgttt
    97981 tcttgaacgt tcggcggcga aagttggcaa agtgactcag gatcacttct ggctgacgtt
    98041 gcgtactctt ttctggtcga ttctcgtcgc gtcaccgtta ccggtgctgt ggatgacgct
    98101 gggttacggc ttgcgcgagg cgtggcctta tccgctggcg gtcgcgattg gcgatggtgt
    98161 aacggccacc gtgccgctgc tgtgggtagt gatgatttgc gccacctttg cccgcccgaa
    98221 cggcttgttt atcgctcatt ttggctggcc gcgcgaacgt gtttcccgtg ggatgcgcta
    98281 ctacctgatg agcatcgggc ttattgtgcc gctgattatg gcgctgatga tgttcgataa
    98341 cctcgacgac cgtgaattct ccggttcgct gggacggctt tgctttatcc tcatttgcgg
    98401 tgcgctggcg gtggtcaccc tcagcctgaa aaaggccggg atcccactgt atctcaacaa
    98461 agagggcagc ggcgacaaca ttaccaacca tatgctgtgg aacatgatga ttggcgcgcc
    98521 actggttgcc attctggcgt cggcggtggg ttatctggca acggcacagg cgctgttagc
    98581 gaggcttgaa acctcggttg ccatctggtt cctgctactg gtggtttatc acgttatccg
    98641 ccgctggatg ctgatccaac gccgcaggct ggcgtttgac cgggcgaagc atcgccgggc
    98701 agagatgtta gcgcaacgtg cgcgtggcga agaggaagca catcatcaca gtagcccgga
    98761 aggggcaatt gaagtcgatg aaagcgaagt cgatctcgat gccatcagtg cgcaatcctt
    98821 gcggctggtg cgctcaattt tgatgttgat cgccctgctt tcggtcattg tgctgtggtc
    98881 agaaatccat tccgctttcg gcttcctcga aaatatttcg ctgtgggatg tcacctccac
    98941 ggtacagggc gtagaaagtc tggagccaat taccctcggt gcggtgctga ttgccattct
    99001 ggtgtttatc atcaccacgc agctggtgcg caacctgccc gcgctgctgg aactggcgat
    99061 tttgcagcac ctggatttaa cgccgggtac gggttacgcc atcaccacca tcaccaaata
    99121 tctgctgatg ctgattggcg ggctggtcgg cttctcaatg attggtattg agtggtcgaa
    99181 attgcagtgg ctggttgccg cgctcggtgt tggtctcggt tttggtttac aggaaatttt
    99241 cgccaacttt atctctggcc tgatcatcct gttcgaaaaa ccgattcgca ttggcgatac
    99301 ggtaacgatc cgcgatctta ctggtagcgt gacgaaaatt aatacccgcg caaccaccat
    99361 cagcgactgg gaccgtaaag agataatcgt gccgaacaag gcgtttatta ccgagcagtt
    99421 tatcaactgg tcgctctctg actcggtcac gcgcgtggtg ttgacgattc cggcccctgc
    99481 cgatgccaac agtgaagaag tgacggaaat cctgctcacc gcagcgcgtc gctgctcgct
    99541 ggtgatcgac aacccggcac cggaagtctt cctggtggat ctgcaacaag ggattcagat
    99601 tttcgagctg cgtatttacg ccgctgagat gggtcaccgt atgccgctac gccatgagat
    99661 ccaccagctg attctggcag gcttccatgc ccacggtatc gatatgccat tcccgccctt
    99721 ccagatgcgt ctggaaagcc tgaatggtaa acaaacgggg agaacattaa cttctgcggg
    99781 caaaggtcgt caggcgggaa gtttgtaatg catgaagctg ccaaatgatg tcaacgtggc
    99841 tcatttagca gcttaacatc atcttcagtt atcaccggcc ctggagagta tcatccatac
    99901 gcattacttc ctgacaactc atcgccggag agacgatgag ttgcaggatt tcatatgatc
    99961 agtcgagagc taactcaggt ttattcgcca tgcgactgca atagttctgg taaatcgcca
   100021 tcgctaacag cgagaagaag acagggccgc caatcatcca caatgtgctg tcccagtctc
   100081 cagcttcaac cacaggttga atgatggtga agacgttcgc aaatgtcacc accagtacga
   100141 caaccaccgt cgcgatcatt gccgacatac gcgttttgaa aatcacaaat ggtctgtcga
   100201 gatcctgacg tgctttaaag aatgggaaag ccagcgcgag gaacaggtaa ggaagcgtca
   100261 tagacacgtt cgccatcagc gtcagcttgt taaagaacgc cgatgcggta ccgccaccaa
   100321 acgaaaccag cagaatgaag atagtaacca acccgcactg catccacatg gcgatagacg
   100381 gcatccccat cgcattcagg cgcgtcatcg gttccggcca caatgctttc ggcgtcccct
   100441 ggatgatggc tttcaacggt gaatagcaaa gcgtaaagaa cgcaccggta taggcgagga
   100501 acatcgaaag tccagtaata cgcgcaaacc atacgcccag cgacaatgac gcttcaggtg
   100561 acaaatgcag tgcattaccc agcgtcatcc caaggctctt catcagcaca taggtaatat
   100621 tgccgaggtt aacggaacca ttacttaata cctgctgcca gtttgtgctg acgccccata
   100681 aaaatattgc cagcgaataa ccgattgaaa taacaatagc ggcaaaaaca atacctttgg
   100741 caaagttctt ttctggattt tccgttttat cgaccagacc accaaccgct tcaattccgc
   100801 cataggcaaa aatggcaaat accacaaacg atagcattgc cagaccggac tgataacccg
   100861 ggttcggtga tgcaaggaaa ttaatatcct gcgcgaaatg cccaccattt aataacaaaa
   100921 tagtaatgct tactaacagc aatactaaat tcagacacat tactgcaata ccgcccaccg
   100981 cagtaatgcg ggcaatttta ttaatcccct tagaagcaac gacggtgacc agaatcatcc
   101041 atgccactgc cagcagacca accacctgcg taggctccag tccggcaata cgccagtgct
   101101 gggtcatgtc gctaccatag aggaatgttg agaacggtac ccaaactttc gcggaggtac
   101161 tcaccatcca gatgatataa gaggaaaacc acataaacgt accaatgaag gcaaaacgtg
   101221 gtccgacact attattcatc caggaataga taccaccttc ttctttgcga taagcagctc
   101281 ccatttcagc catcattaag gcgaatggaa taaagaataa caatgcagaa aatatataaa
   101341 agggaatcgc actataaccc attaagtaat aagccgatgg gctattggca aaaccaaata
   101401 cggaagtaaa gatcatcaat atgagtccta tcagactcat tttttttatc gtgtgaggca
   101461 tgaaaccatc cttcatttca gcgcgtgtat aacacgtgcc cactgaactg cactgcgcaa
   101521 cattgagcag gcagcgtaaa aaccaataca gaattgatac ctggatgata tcaaatatcc
   101581 agtcttaaat tgtggccttt agcggaaaaa tgaaaacata tgctacagaa tggcgcttat
   101641 tacgccattc ttcgctgtta attcagtaat ttttcagaat tatgcccggt caacgctaaa
   101701 ggcgatgact tcagccagtg tctccgcgcc cagcgccaac atcaccagac gatcaacacc
   101761 taatgccacg ccggaacagt caggcatacc gactttcaag gcgtcaatca gattctggtc
   101821 aatggggtgc tgcggcaaac cgcgcgccgc gcgcttacgg ttatcttgtt caaagcgttg
   101881 ttgctgctca cgggcatccg tcaattcatg gaaaccattc gccagctcaa tacctttata
   101941 ataaacctca aagcgttcag cgacccgatg atcttcggta ctgatttgcg ccagtgatgc
   102001 ctggctggct ggaaagtggt acacaaaggt cggtttttct ttaccaatat ttggctctac
   102061 gccaaaggta aacagcaatt gtagcaacgt gtcgcggtct tcttcggtat cagcaacatt
   102121 gctcaaatcc agtttcgctg ccacttcccg cagttgcgtt ttgtcggcag agagtgggtc
   102181 aatttccaga taacgcaaga aagcttgttg ataagaaagg ctttctgctg ccgggcagtc
   102241 cagcacctgt tgtaagagat cgtccacctc gttcatcaac cggtacatat catagtgcgg
   102301 tcgataccac tccagcatag tgaactcagg gttgtgataa cgccccatct cttcattacg
   102361 gaagctgcgg cacagctgga ataccggccc acaaccggca accagcaggc gtttcatatg
   102421 gtattccggg ctggtcatta accagagatt catcccctgc gaatgcccgg ggccaacgaa
   102481 acgtgtctca aacgggacca aatgaatatc ggttaccgtc gcctggctca tacaaggcgt
   102541 ctccacctcc agcactccac gatcggcaaa gaaacgacgg atctccgcca taatcgccgc
   102601 gcgttttaat aagttaggaa tggatgcgct cggctgccag gatgccgttt cgctcatagt
   102661 taaatctcca gtttttgaca agggcacgaa gtctactcgc aacgcgacgg cgagacaaat
   102721 tttacgcagg aatcaaacag cggtgggcag tgactaaaaa aagcacgatc tgatggttta
   102781 gtaattaaat taatcatctt cagtgataat ttagccctct tgcgcactaa aaaaatcgat
   102841 ctcgtcaaat ttcagactta tccatcagac tatactgttg tacctataaa ggagcagtgg
   102901 aatagcgttc gcagaccgta actttcaggt acttaccctg aagtacgtgg ctgtgggata
   102961 aaaacaatct ggaggaatgt cgtgcaaacc tttcaagccg atcttgccat tgtaggcgcc
   103021 ggtggcgcgg gattacgtgc tgcaattgct gccgcgcagg caaatccgaa tgcaaaaatc
   103081 gcactaatct caaaagtata cccgatgcgt agccataccg ttgctgcaga agggggctcc
   103141 gccgctgtcg cgcaggatca tgacagcttc gaatatcact ttcacgatac agtagcgggt
   103201 ggcgactggt tgtgtgagca ggatgtcgtg gattatttcg tccaccactg cccaaccgaa
   103261 atgacccaac tggaactgtg gggatgccca tggagccgtc gcccggatgg tagcgtcaac
   103321 gtacgtcgct tcggcggcat gaaaatcgag cgcacctggt tcgccgccga taagaccggc
   103381 ttccatatgc tgcacacgct gttccagacc tctctgcaat tcccgcagat ccagcgtttt
   103441 gacgaacatt tcgtgctgga tattctggtt gatgatggtc atgttcgcgg cctggtagca
   103501 atgaacatga tggaaggcac gctggtgcag atccgtgcta acgcggtcgt tatggctact
   103561 ggcggtgcgg gtcgcgttta tcgttacaac accaacggcg gcatcgttac cggtgacggt
   103621 atgggtatgg cgctaagcca cggcgttccg ctgcgtgaca tggaattcgt tcagtatcac
   103681 ccaaccggtc tgccaggttc cggtatcctg atgaccgaag gctgccgcgg tgaaggcggt
   103741 attctggtca acaaaaatgg ctaccgttat ctgcaagatt acggcatggg cccggaaact
   103801 ccgctgggcg agccgaaaaa caaatatatg gaactgggtc cacgcgacaa agtttctcag
   103861 gccttctggc acgaatggcg taaaggcaac accatctcca cgccgcgtgg cgatgtggtt
   103921 tatctcgacc tgcgtcacct cggcgagaaa aaactgcatg aacgtctgcc gttcatctgc
   103981 gaactggcga aagcgtacgt tggcgtcgat ccggttaaag aaccgattcc ggtacgtccg
   104041 accgcacact acaccatggg cggtatcgaa accgatcaga actgtgaaac ccgcattaaa
   104101 ggtctgttcg ccgtgggtga atgttcctct gttggtctgc acggtgcaaa ccgtctgggc
   104161 tccaactccc tggcggaact ggtggtcttc ggccgtctgg ccggtgaaca agcgacagag
   104221 cgtgcagcaa ctgccggtaa tggcaacgaa gcggcaattg aagcgcaggc agctggcgtt
   104281 gaacaacgtc tgaaagatct ggttaaccag gatggcggcg aaaactgggc gaagatccgc
   104341 gacgaaatgg gcctggcaat ggaagaaggt tgcggtatct accgtacgcc ggaactgatg
   104401 cagaaaacca tcgacaagct ggcagagctg caggaacgct tcaagcgcgt gcgcatcacc
   104461 gacacctcca gcgtgttcaa caccgacctg ctctacacca ttgaactggg ccacggtctg
   104521 aacgttgctg aatgtatggc gcactccgca atggcacgta aagagtcccg cggcgcacac
   104581 cagcgtctgg acgaaggttg caccgagcgt gacgacgtca acttcctcaa acacaccctc
   104641 gccttccgcg atgctgatgg cacgactcgc ctggagtaca gcgacgtgaa gattactacg
   104701 ctgccgccag ctaaacgcgt ttacggtggc gaagcggatg cagccgataa ggcggaagca
   104761 gccaataaga aggagaaggc gaatggctga gatgaaaaac ctgaaaattg aggtggtgcg
   104821 ctataacccg gaagtcgata ccgcaccgca tagcgcattc tatgaagtgc cttatgacgc
   104881 aactacctca ttactggatg cgctgggcta catcaaagac aacctggcac cggacctgag
   104941 ttaccgctgg tcctgccgta tggcgatttg tggttcctgc ggcatgatgg ttaacaacgt
   105001 gccaaaactg gcatgtaaaa ccttcctgcg tgattacacc gacggtatga aggttgaagc
   105061 gttagctaac ttcccgattg aacgcgatct ggtggtcgat atgacccact tcatcgaaag
   105121 tctggaagcg atcaaaccgt acatcatcgg caactcccgc accgcggatc agggtactaa
   105181 catccagacc ccggcgcaga tggcaaagta tcaccagttc tccggttgca tcaactgtgg
   105241 tctgtgctac gccgcgtgcc cgcagtttgg cctgaaccca gagttcatcg gtccggctgc
   105301 cattacgctg gcgcatcgtt ataacgaaga tagccgcgac cacggtaaga aggagcgtat
   105361 ggcgcagttg aacagccaga acggcgtatg gagctgtact ttcgtgggct attgctccga
   105421 agtctgcccg aaacacgtcg atccggctgc ggccattcag cagggcaaag tagaaagttc
   105481 gaaagacttt cttatcgcga ccctgaaacc acgctaagga gtgcaacatg acgactaaac
   105541 gtaaaccgta tgtacggcca atgacgtcca cctggtggaa aaaattgccg ttttatcgct
   105601 tttacatgct gcgcgaaggc acggcggttc cggctgtgtg gttcagcatt gaactgattt
   105661 tcgggctgtt tgccctgaaa aatggcccgg aagcctgggc gggattcgtc gactttttac
   105721 aaaacccggt tatcgtgatc attaacctga tcactctggc ggcagctctg ctgcacacca
   105781 aaacctggtt tgaactggca ccgaaagcgg ccaatatcat tgtaaaagac gaaaaaatgg
   105841 gaccagagcc aattatcaaa agtctctggg cggtaactgt ggttgccacc atcgtaatcc
   105901 tgtttgttgc cctgtactgg taaggagcct gagatgatta atccaaatcc aaagcgttct
   105961 gacgaaccgg tattctgggg cctcttcggg gccggtggta tgtggagcgc aattattgcg
   106021 ccggtgatga tcctgctggt gggtattctg ctgccactgg ggctgtttcc gggtgatgcg
   106081 ctgagctacg agcgcgttct ggcgttcgcg cagagcttca ttggtcgcgt attcctgttc
   106141 ctgatgatcg ttctgccgct gtggtgtggt ttacaccgta tgcaccacgc gatgcacgat
   106201 ctgaaaattc acgtgcctgc gggcaaatgg gttttctacg gtctggctgc tatcctgaca
   106261 gttgtcacgc tgattggtat cgttacaatc taacgtatcg ccaatgtaaa tccggcccgc
   106321 ctatggcggg ccgttttgta tggaaaccag accttatgtt caaaacgacg ctctgcgcct
   106381 tattaattac cgcctcttgc tccacatttg ctgcccctca acaaatcaac gatattgtgc
   106441 atcgcacaat taccccgctt atagagcaac aaaagatccc cggtatggcg gtggcggtaa
   106501 tttatcaggg taaaccttat tactttacct ggggctatgc ggacatcgcc aaaaagcagc
   106561 ccgtcacaca gcaaacgttg tttgagttag gttcggtcag caaaacattt acgggcgtgc
   106621 ttggtggcga cgctattgct cgaggggaaa tcaagttaag cgatcccgca acaaaatact
   106681 ggcctgaact taccgctaaa cagtggaatg ggatcacact attacatctt gcgacctaca
   106741 ccgctggcgg cctgccattg caggtgccgg atgaagtgaa atcctcaagc gacttgctgc
   106801 gcttctatca aaactggcag cctgcatggg ctccaggaac acaacgtctg tatgccaact
   106861 ccagtatcgg tttgttcggc gcactggctg tgaagccgtc tggtttgagt tttgagcagg
   106921 cgatgcaaac tcgtgtcttc cagccactca aactcaacca tacgtggatt aatgtacctc
   106981 ccccagaaga aaagaattac gcctggggat atcgcgaagg taaggcagtg catgtttcgc
   107041 caggggcgtt agatgctgaa gcttatggtg tgaagtcgac cattgaagat atggcccgct
   107101 gggtacgaag caatatgaat ccccgtgata tcaacgacaa aacacttcag caagggatac
   107161 aactggcaca atctcgctac tggcaaaccg gcgatatgta tcagggcctg ggctgggaaa
   107221 tgctggactg gccggtaaat cctgacagca tcattaacgg cagtggcaat aaaattgcac
   107281 tggcagcaca ccctgtaaaa gcgattacgc ccccaactcc tgcagtacgc gcatcatggg
   107341 tacataaaac aggggcaacc ggcggatttg gtagctatgt cgcgtttatt ccagaaaaag
   107401 agctgggtat agtgatgctg gctaacaaaa actatcccaa tccagcgaga gtcgccgccg
   107461 cctggcagat tctcaacgct ctacagtaaa atttcatcgg gtccgaattt tcggaccttt
   107521 tctgcgcttt tccttgctgt catctacact tagaaaaaaa ccagtaagga aacatttatg
   107581 cgcctgctcc ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc ctgcagttct
   107641 cctacgccgc cgcgtggcgt aacagtagtg aataatttcg acgcaaaacg ttatctcggt
   107701 acctggtatg agattgcccg ttttgatcac cgttttgaac gtggactgga aaaagtcacc
   107761 gcaacataca gtctgcgtga tgacggcggc ctgaatgtca tcaataaagg ctataaccct
   107821 gacagagaaa tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc accaacacgc
   107881 gctgcgctga aagtgtcatt ctttggtcct ttctatggcg gttataacgt cattgcactt
   107941 gatcgggaat accgccatgc gctggtttgc ggcccggacc gcgactacct gtggatactc
   108001 tcccgcacgc caaccatttc tgacgaagtg aaacaggaga tgctggcagt cgcgacccgg
   108061 gaagggtttg atgtcagtaa gtttatttgg gtacagcagc ccggtagtta gtgagtgctg
   108121 agtttcagac caataatccc caatacgatt aacgccagac tcgccaggcg catcgggtta
   108181 gcggactcac cgagcagcac aatgccggtg atggccgcgc cgactgcgcc aatacccgtc
   108241 cacacggcat aagccgtccc taccggtaac gatttcatcg cccaggcaag tagcgccata
   108301 ctgactatca tcgccgtcac ggtaataaca ctcggcgtca aacggctaaa gccgtgggta
   108361 tatttcaggc caacggccca taccacttcc agcagaccag caataactaa gataatccag
   108421 gacatatcag gctccggaga attggggccg tccccggtga aaagaggcgt ttgcaggtcg
   108481 tcctgcaaag ctgatggttg aacggatatt ttgccaggtg gcaggggaat ttcaactatt
   108541 acctttgtga ctatcgcaaa aaaacaaacg gcacgacata actgtcgtac cgttattgct
   108601 tattgctgcg ctttcgttgc tgcaccagaa atcgcgttac cgccatcaga aatgtcttca
   108661 ccaacgccac gcgtggtgtt gcaggcagtt aatactgttg aaagcaccag aacagaaaag
   108721 atcgctgcaa ttgtcttctt caccataacg tcttcctttt aggtaatgtt atttatgttt
   108781 gcctatagca aattaataat agacaacatc gccaaaaatg atggaaatct gatgattttt
   108841 cggaagagaa gaaaaattag ctggcagcgc gggagatgga gttgccgaga tgtttgatgt
   108901 cttcgccgaa accgcgagcg gtgttacagc ccgtgagcag cgtgctggca agcaaaacaa
   108961 gaacgataag gcgtttcatc atctctgacc tgaataagtg atggtgcagc ctgcaggccg
   109021 caccacaacc gcattacttc acgcgagaga cgtattcacc agagcgggta tccactttga
   109081 tgacttcgcc gatttgtacg aacagcggaa ctttaaccac agcgccagta gacagggtag
   109141 ccggtttgcc gccagtacct gcggtatcac ctttcaggcc cggatcggta tcaacgattt
   109201 ccagttcaac gaagttcggc ggagtaacgg agatcggctg accattccac agagttacga
   109261 tacactctgc ctgatccagc agccatttag cgttgtcgcc aattgctttt gcatcagcag
   109321 acagctgctc gaaagtttcg ttgttcatga agtgccagaa ctcaccgtcg ttgtacaggt
   109381 aagtcaggtt catatcgaca acatcagcgc cttcagcgga atcagtagat ttgaaggttt
   109441 tttctacgcg agtaccggtc agcagacgac gcagtttaac gcgagcaaat gcctggcctt
   109501 tacccggttt tacgaattca ctcgcttcaa ccgcgtaagg ttcgccgtct aacatgattt
   109561 taagaccagc acgaaaatcg ttgctatagt acgttgccat aaggccctct gaaatttgtt
   109621 aattggtagc taagccacaa aatggcgcat attgtaaccc taaatacccc atccagagaa
   109681 gattggttaa cgcaacttgc cgatgttgtg accgatcctg atgaacttct gcgtcttttg
   109741 aatatagacg cggacgaaaa actgttggcc ggacgcagcg ccaaaaagct gtttgccctg
   109801 cgtgtgcccc gctcatttat cgatcgcatg gagaaaggca atccggacga tcctcttttg
   109861 cgtcaggtac ttacctcgca agatgagttt gtcgtcgcgt ccggattctc caccgacccg
   109921 ctggaagaac agcacagcgt agtgcctggt ttgttgcata aataccacaa ccgggcgctt
   109981 ttgctggtca aaggcggctg cgcggtaaat tgccgctatt gcttccgtcg ccacttcccc
   110041 tatgccgaaa atcagggcaa caagcgtaac tggcagactg ctcttgagta tgttgctgcg
   110101 catccggaac tggacgagat gattttctcc ggcggcgatc cgctgatggc gaaagatcac
   110161 gagctggact ggttgctcac acaactggaa gccatcccgc atatcaaacg tctgcggatt
   110221 cacagccgtc tgccgattgt gatcccggca cgtatcaccg aggcgctggt tgaacgcttt
   110281 gcccgttcta cgctgcaaat cttgctggtg aatcacatca atcatgccaa tgaggtagat
   110341 gaaacgttcc gtcaggcgat ggctaaattg cgccgtgtcg gtgtcaccct gcttaaccag
   110401 agcgttctgt tacgtggtgt gaacgataac gcacaaacgc tggcaaacct gagtaatgcg
   110461 ttgttcgatg ccggcgtaat gccgtattac ctgcatgtgc tcgataaagt acagggcgcg
   110521 gcgcatttta tggtgagtga tgacgaagca cggcagatta tgcgtgagtt gctgacactg
   110581 gtgtcgggat atctggtgcc gaaactggcg cgagaaattg gcggcgaacc cagcaaaacg
   110641 ccgctggatc tccagctacg ccagcagtaa ctgattaaac agggaagtca aacattaaaa
   110701 ttgcctgatg cgctacgctt atcaggccta catcgtgcat tgaaattgaa tggatttgca
   110761 atgaactgta ggccggataa ggcgtttacg ccgcatccgg caagagcaac aaacgcaagt
   110821 acagcaacct gtcattaaac tgggaaattt aatctttgcc cgttgcataa cctttaatta
   110881 ttcatataca ttgctgttgt tactctttat ataactaaca ccaataatta acctttcttt
   110941 tctgtttata tagagtgagt acggctattt atgttatttt aacaactatt actaccaagc
   111001 ccatttatct ggcttataaa ttaaaattaa ttatttaatt gagaaatggt tagggagaac
   111061 ctacatggcc ataagcatca agggtgttaa taccggtgtt attcgcaaga gtaataattt
   111121 cattgcattg gcgctgaaaa ttaaagaacc tcgcaataaa gaatcgttgt tctttatgtc
   111181 tgtcatggaa ctgcgcgacc tgctgattgc gctggaaagt cgtctgcatc aaaaacataa
   111241 actggatgct gctgcacgcc ttcagtatga gcaagctcgc gataaagtca ttaagaaaat
   111301 ggcagaaaac atcccagaaa ttctggttga tgagcttaaa aatgccgata ttaatcgtcg
   111361 ggttaacacg ctggaactta ccgataatca aggcgaaaat cttaccttcg tattaacgct
   111421 acatgacggt aacaaatgtg agttagtcgt caatgagttg cagattgaaa tgctggcgcg
   111481 agcaatcatt catgcaatta ataatgcgga aatgcgtgaa ctggcattac gtattacctc
   111541 actgttagat ttcctgccgt tatatgacgt tgactgccag gaaaatggca atctggaata
   111601 tgacacctat tcccaaccgg aatggaaaca taacttgttt gaccattatc tggcggtgct
   111661 ttaccgcttt aaagatgaaa gtggcaaaga acaatttagc ggcgcagtgg tgaaaacgcg
   111721 tgaagcaacg ccgggtaaag aagtagaggc gatcactcgc cgaatgcttg atttcagccc
   111781 acgactgaaa aaactcgtgg gtgtgccatg tcaggtctat gttcgcaccg tagcggcgaa
   111841 taacgcccaa ccgctaaccc aggatcaatg cttacgcgcg ctgcatcact tacgtgttca
   111901 atccaccagc aaaactgcac cacaggcgaa ataatctcat ctttgctaat gcgaggctgg
   111961 agccatccag cctgaaatga ctgataacgt gatcgttggt catgccggat gcggcgtaaa
   112021 cgccttatcc ggcctacaaa ttctcaagca ttcaatcagt tgcaatgcat tatgtaggcc
   112081 tgataagcga agcgcatctg gcaattatac gtttgtcacc atcacatcag ttcgggcact
   112141 tataaacctg gccgataatc tggctatccg tcgggacaaa actggacaac attccctgcg
   112201 acgggctact gatgccataa atcacgttac cgcccattgc agccgcctgg ttgcgcagat
   112261 cgtttgctgc gccgcgcata gaaccgcctt cttctccgtg ttgcccggaa agccagttgc
   112321 tttgcttacc tgtcgcagta ccaatcagct ggcactctgc gcctggctgc tcgtccacaa
   112381 tgcgtacact ctgaccggca gcactcaatt cgttgctgga gctacaaccc gccatcagta
   112441 gcgcggcacc gacaatccct gctaagtatt ttacgtgcat gttattcccc ataatcaatg
   112501 agctggacgc cactcgcgtc gtccgtgtct gaatcttata ctagaaagat gaccaaaaga
   112561 aaaaccccca gacatttctg cccgggggtt tgtttatttc tgcgaggtgc agggcaatta
   112621 catcatgccg cccatgccac ccatgccgcc cataccgcca gcagcgccta agtcagctgc
   112681 atcatttttc ggcaggtcgg taaccatgca ctcggtggtg atcatcaggc cagccacaga
   112741 agccgcgtac tgcagagcag aacgggttac tttggttggg tccaggatac ccatgtcgat
   112801 catgttgccg tattcttcgg ttgctgcgtt gtaaccgtag ttgccgtcgc cgcctttaac
   112861 ggtgttagca acaacagacg gttcttcgcc gcagttcagg acgatctgac gcagcggagc
   112921 ttccattgca cgcagtgcaa ctttgatacc cacgttctgg tcttcgttct gaccacgcag
   112981 gtcagccagt ttagacgcta cgcggatcag cgcaacacca ccaccagcaa ccacgccttc
   113041 ttctaccgca gcacgggtcg cgtgcagggc gtcttcaacg cgtgcttttt tctctttcat
   113101 ttcaacttcg gtagcagcac ctactttgat aactgcaacg ccgcctgcca gtttcgctac
   113161 gcgttcctgc agtttttcac ggtcgtagtc agaagttgct tcttcaatct gctgacggat
   113221 ctgagcaaca cggccctgga ttgcagcttc ttcacccacg ccatcgatga tagtggtggt
   113281 gtctttgttg atcacaacac gtttagcctg acccaggtct tccagggttg ctttttccag
   113341 ctccataccg atctcttcag agattacggt accgccagtc agggttgcga tatcctgcag
   113401 catagcttta cgacgatcgc cgaagcccgg agctttaacc gcagcaactt tcacgatgcc
   113461 acgcatggtg ttaacaacca gagttgccag cgcttcgcct tcaacatctt cagcgatgat
   113521 cagcagcggt ttgcctgctt tggcaacagc ttccagaacc ggcagcattt cgcggatgtt
   113581 agagattttc ttgtcagcca gcaggatgaa cgggctttcc agttctactg cgccagtttc
   113641 cggcttgttg atgaagtaag gagacaggta gccacggtcg aactgcatac cttcaaccac
   113701 gtccagttcg tcctgcagac cggtaccgtc ttcaacggtg ataacgcctt ctttaccgac
   113761 tttgtccatc gcttcagcga tcagtttacc tacggtttcg tcggagttag cggagatggt
   113821 accaacctga gcaatcgctt tagagtcaga gcacggtacg gacagcgctt tcagttcttc
   113881 aactgcagcg gtaacggctt tgtcgatacc acgtttcagg tccatcgggt tcatgcccgc
   113941 agcaacagct ttcagaccct cagtgatgat agcctgagcc agtacagttg cagtggtggt
   114001 accgtcgcct gcagcgtcgt tcgctttaga ggcaacttct ttcaccatct gcgcacccat
   114061 attttcgaac ttgtcttcca gttcgatttc acgagcaacg gaaacaccat ctttggtgat
   114121 ggtcggtgca ccgaaagatt tatccagaac tacgttacgg cctttcggac cgagggtaac
   114181 tttcactgca tctgccagta cgtttacgcc gcgcagcatt ttcacacgag cgtcgttacc
   114241 gaattttacg tctttagctg ccattatctt tattccttaa attcgtatgt tcagtgtcgt
   114301 gcgaggatta cgcttcaaca attgccagaa tgtcgctttc ggacatgatc aacacttctt
   114361 cattgtcgat cttctcagat ttcacaccgt agccatcgtt gaaaataacg atgtcgccaa
   114421 ctttcacatc cagcggcttc acttcgccat tttcaaggat acggccattg ccgacagcca
   114481 gcacttcgcc gcgggtggat ttagccgctg cagagccggt cagaacgatg ccgccagcag
   114541 atttagtttc aacttcttta cgcttgacga tcacgcgatc atgcaatgga cgaatattca
   114601 ttgataactc tcctttgaga aagtccgtat ctgttatggg tgacgccgga gcttacgtgg
   114661 tttcccggct ggtgaccaga gaaatgggga tgaggattcg ccccttcaag ggggaaaaag
   114721 aaaaaaaatt ctgatcagca caaaatcggg tgaaaaccct gattcacctc acatttcatc
   114781 gcaatttctt catcgccgtc ataagcgaat ctgattgtgc taccatcgaa aatctacgca
   114841 gttgcccaaa atttgggcgc aatcggatat caacccatga gtggactcaa acaagaactg
   114901 gggctggccc agggcattgg actgctatcg acgtcattat taggcactgg cgtgtttgcc
   114961 gttcctgcgt tagctgcgct ggtagcgggc aataacagcc tgtgggcgtg gcccgttttg
   115021 attatcttag tgttcccgat tgcgattgtg tttgcgattc tgggtcgcca ctatcccagc
   115081 gcaggcggtg tcgcgcactt cgtcggtatg gcgtttggtt cgcgacttga gcgagtcacc
   115141 ggctggttgt ttttatcggt cattcccgtg ggtttgcctg ccgcgctaca aattgccgcc
   115201 gggttcggcc aggcaatgtt tggctggcat agctggcaac tgttgttggc agaactcggt
   115261 acgctggcgc tggtgtggta tatcggtact cgcggtgcca gttccagtgc taatctacaa
   115321 accgttattg ccggacttat cgtcgcgctg attgtcgcta tctggtgggc gggcgatatc
   115381 aaacctgcga atatcccctt cccggcacct ggtaatatcg aacttaccgg gttatttgct
   115441 gcgttatcag tgatgttctg gtgttttgtc ggtctggaag catttgccca tcttgcctcg
   115501 gaatttaaaa atccagagcg tgattttcct cgcgcattga tgattggtct gctgctggca
   115561 ggattagtct actggggctg tacggtagtc gtcttacact tcgacgccta tggtgaaaaa
   115621 atggcggcgg cagcatcgct tccaaaaatt gtagtgcagt tgttcggtgt aggagcgtta
   115681 tggattgcct gcgtgattgg ctatctggcc tgctttgcca gtctcaacat ttatatacag
   115741 agcttcgccc gcctggtctg gtcgcaggcg caacataatc ctgaccacta cctggcacgc
   115801 ctctcttctc gccatatccc gaataatgcc ctcaatgcgg tgctcggctg ctgtgtggtg
   115861 agcacgttgg tgattcatgc tttagagatc aatctggacg ctcttattat ttatgccaat
   115921 ggcatcttta ttatgattta tctgttatgc atgctggcag gctgtaaatt attgcaagga
   115981 cgttatcgac tactggcggt ggttggcggg ctgttatgcg ttctgttact ggcaatggtc
   116041 ggctggaaaa gtctctatgc gctgatcatg ctggcggggt tatggctgtt gctgccaaaa
   116101 cgaaaaacgc cggaaaatgg cataaccaca taatccggcg tttcgacatt aatcctggcg
   116161 atcgtcttta tgatcaaggc ggtcgcgctc atcatccttt cgctggtact caccatcaaa
   116221 agtattaccg ccaccggtcc cggcgctaaa accgccgcct ggcatacgag aaaagcgcaa
   116281 atgcggcatc aacttcactg tcagatgctt ttgcaccggc ggtaataaaa gcagaagacc
   116341 gaggaagtcg gtgaaaaagc ccggtaataa aagcagcaaa ccggcaatga tcagcgaaac
   116401 acttttaatc atctccgccg ctgggttttc acccgccgcc attttttgct gcatcagcgc
   116461 gaaattctta aagccctggt tacgtaccag tgacatcccg ataactgacg tgaatataac
   116521 cagcacgagg gtcagcaata cccccaatac atgggcaacc tgaataaaga ttgaaatctc
   116581 aatatagaca taaaggaaaa tggcaataaa aggtaaccag cgcaaaggtt tctcctgtaa
   116641 tagcagccgg taaaacccgg ctacctgaat gggttgcgaa tcgcgttcag cttatattgt
   116701 ggtcattagc aaaatttcaa gatgtttgcg caactatttt tggtagtaat cccaaagcgg
   116761 tgatctattt cacaaattaa taattaaggg gtaaaaaccg acacttaaag tgatccagat
   116821 tacggtagaa atcctcaagc agcatatgat ctcgggtatt cggtcgatgc aggggataat
   116881 cgtcggtcga aaaacattcg aaaccacata tattctgtgt gtttaaagca aatcattggc
   116941 agcttgaaaa agaaggttca catgtcaaac aacattcgta tcgaagaaga tctgttgggt
   117001 accagggaag ttccagctga tgcctactat ggtgttcaca ctctgagagc gattgaaaac
   117061 ttctatatca gcaacaacaa aatcagtgat attcctgaat ttgttcgcgg tatggtaatg
   117121 gttaaaaaag ccgcagctat ggcaaacaaa gagctgcaaa ccattcctaa aagtgtagcg
   117181 aatgccatca ttgccgcatg tgatgaagtc ctgaacaacg gaaaatgcat ggatcagttc
   117241 ccggtagacg tctaccaggg cggcgcaggt acttccgtaa acatgaacac caacgaagtg
   117301 ctggccaata tcggtctgga actgatgggt caccagaaag gtgaatatca gtacctgaac
   117361 ccgaacgacc atgttaacaa atgtcagtcc actaacgacg cctacccgac cggtttccgt
   117421 atcgcagttt actcttccct gattaagctg gtagatgcga ttaaccaact gcgtgaaggc
   117481 tttgaacgta aagctgtcga attccaggac atcctgaaaa tgggtcgtac ccagctgcag
   117541 gacgcagtac cgatgaccct cggtcaggaa ttccgcgctt tcagcatcct gctgaaagaa
   117601 gaagtgaaaa atatccaacg taccgctgaa ctgctgctgg aagttaacct tggcgcaaca
   117661 gcaatcggta ctggtctgaa cacgccgaaa gagtactctc cgctggcagt gaaaaaactg
   117721 gctgaagtca ctggcttccc atgcgtaccg gctgaagacc tgatcgaagc gacctctgac
   117781 tgcggcgctt atgttatggt tcacggcgcg ctgaaacgcc tggctgtgaa gatgtccaaa
   117841 atctgtaacg acctgcgctt gctctcttct ggtccacgtg ccggcctgaa cgagatcaac
   117901 ctgccggaac tgcaggcggg ctcttccatc atgccagcta aagtaaaccc ggttgttccg
   117961 gaagtggtta accaggtatg cttcaaagtc atcggtaacg acaccactgt taccatggca
   118021 gcagaagcag gtcagctaca gttgaacgtt atggagccgg tcattggcca ggccatgttc
   118081 gaatccgttc acattctgac caacgcttgc tacaacctgc tggaaaaatg cattaacggc
   118141 atcactgcta acaaagaagt gtgcgaaggt tacgtttaca actctatcgg tatcgttact
   118201 tacctgaacc cgttcatcgg tcaccacaac ggtgacatcg tgggtaaaat ctgtgccgaa
   118261 accggtaaga gtgtacgtga agtcgttctg gaacgcggtc tgttgactga agcggaactt
   118321 gacgatattt tctccgtaca gaatctgatg cacccggctt acaaagcaaa acgctatact
   118381 gatgaaagcg aacagtaatc gtacagggta gtacaaataa agaaggcacg tcagatgacg
   118441 tgcctttttt cttgtgagca gtaacttaaa aataacaacc taatatcaac ttgttaaaaa
   118501 acaaggaagg ctaatatgct agttgtagaa ctcatcatag ttttgctggc gatcttcttg
   118561 ggcgccagat tggggggaat aggtattggt tttgcaggcg gattgggggt gctggttctt
   118621 gccgctattg gcgttaaacc cggtaacatc ccgttcgatg tcatttccat tatcatggcg
   118681 gttatcgccg ctatttctgc catgcaggtt gctggcggtc tggactatct ggttcatcag
   118741 acagaaaagc tgctgcgccg taacccgaaa tacatcacga tcctcgcacc gatcgtgacc
   118801 tatttcctga ctatctttgc tggtactggc aacatatctc tggcgacact gccagttatc
   118861 gctgaagttg cgaaggaaca aggcgtcaaa ccttgccgtc cgctgtctac tgcagtggta
   118921 tccgcgcaga ttgcgatcac cgcatcgcca atctcagcgg cagtggttta catgtcttcc
   118981 gtgatggaag gtcatggcat cagctacctc catctgctct ccgtggtcat cccgtccacc
   119041 ctactggcgg ttctggtgat gtccttcctg gtcactatgc tgttcaactc caaactctct
   119101 gacgatccga tttatcgtaa gcgtctggaa gagggcctgg ttgaactgcg cggtgaaaag
   119161 cagattgaaa tcaaatccgg tgcaaaaacg tccgtctggc tgttcctgct gggcgtagtt
   119221 ggcgtggtta tctatgcaat catcaacagc ccaagcatgg gtctggttga aaaaccgctg
   119281 atgaacacca ccaacgcaat cctgatcatc atgctcagcg ttgcaactct gaccaccgtt
   119341 atctgtaaag tcgataccga caacatcctc aactccagca ccttcaaagc aggtatgagc
   119401 gcctgtattt gtatcctggg tgttgcgtgg ctgggcgata ctttcgtttc caacaacatc
   119461 gactggatca aagataccgc tggtgaagtg attcagggtc atccgtggct gctggccgtc
   119521 atcttcttct ttgcttctgc tctgctgtac tctcaggctg caaccgcaaa agcactgatg
   119581 ccgatggctc tggcactgaa cgtttctccg ctgaccgctg ttgcttcttt cgctgcggtg
   119641 tctggtctgt tcattctgcc aacctacccg acgctggttg ctgcggtaca gatggatgac
   119701 acgggtacta cccgtatcgg taaattcgtc ttcaaccatc cgttcttcat cccgggtact
   119761 ctgggtgttg ccctggccgt ttgcttcggc ttcttgctgg gtagcttcat gctgtaatga
   119821 ccaatcgcgg ggcgttcacg ccccgctttc tttcccgccg actaacatcc ttcccccgtc
   119881 cgttgtatag tgacctctct cttgcggttc catctgttct tgcgaggtgt ttatgcttga
   119941 tgaaaaaagt tcgaataccg cgtctgtcgt ggtgctatgt accgcaccgg atgaagcgac
   120001 agcccaggat ttagccgcca aagtgctggc ggaaaaactg gcggcctgcg cgaccttgat
   120061 ccccggcgct acctctctct attactggga aggtaagctg gagcaagaat acgaagtgca
   120121 gatgatttta aaaactaccg tatctcacca gcaggcactg ctggaatgcc tgaagtctca
   120181 tcatccatat caaaccccgg aacttctggt tttacctgtt acacacggag acacagatta
   120241 cctctcatgg ctcaacgcat ctttacgctg atcctgctac tttgcagcac ttccgttttt
   120301 gccggattat tcgacgcgcc gggacgttca caatttgtcc ccgcggatca agcctttact
   120361 tttgattttc agcaaaacca acatgacctt aatctgacct ggcagatcaa agacggttac
   120421 tacctctacc gtaaacagat ccgcattacg ccggaacacg cgaaaattgc cgacgtgcag
   120481 ctgccgcaag gcgtctggca tgaagatgag ttttacggca aaagcgagat ttaccgcgat
   120541 cggctgacgc ttcccgtaac catcaaccag gcgagtgcgg gagcgacgtt aactgtcacc
   120601 taccagggct gcgctgatgc cggtttctgt tatccgccag aaaccaaaac cgttccgtta
   120661 agcgaagtgg tcgccaacaa cgcagcgtca cagcctgtgt ctgtttcgca gcaagagcag
   120721 cacaccgcgc aattgccctt ttccgcgctc tgggcgttgt tgatcggtat tggtatcgcc
   120781 tttacgccat gcgtgctgcc aatgtaccca ctgatttctg gcatcgtgct gggtggtaaa
   120841 cagcggctct ccactgccag agcattgttg ctgaccttta tttatgtgca ggggatggca
   120901 ctgacttaca cggcgctggg tctggtagtt gccgccgcag ggttacagtt ccaggcggcg
   120961 ctacagcacc catacgtgct cattggcctc gccatcgtct ttaccttgct ggcgatgtca
   121021 atgtttggct tgtttactct acaactcccc tcttcgctgc aaacacgtct cacgttgatg
   121081 agcaatcgcc aacagggcgg ctcacctggc ggtgtgtttg ttatgggggc gattgccgga
   121141 ctgatctgtt caccatgcac caccgcaccg cttagcgcga ttctgctgta tatcgcccaa
   121201 agcgggaaca tgtggctggg cggcggcacg ctttatctct atgcgttggg catgggcctg
   121261 ccgctgatgc taattaccgt ctttggtaac cgcttgctgc cgaaaagcgg cccgtggatg
   121321 gaacaagtca aaaccgcgtt tggttttgtg atcctcgcac tgccggtctt cctgctggag
   121381 cgagtgattg gtgatgtatg gggattacgc ttgtggtcgg cgctgggtgt cgcattcttt
   121441 ggctgggcct ttatcaccag cctacaggct aaacgcggct ggatgcgcgt ggtgcaaata
   121501 atcctgctgg cagcggcatt ggttagcgtg cgcccacttc aggattgggc atttggtgcg
   121561 acgcataccg cgcaaactca gacgcatctc aactttacac aaatcaaaac ggtagatgag
   121621 ttaaatcagg cgctcgttga agccaaaggc aaaccggtga tgttagatct ttatgccgac
   121681 tggtgcgtcg cctgtaaaga gtttgagaaa tacaccttca gcgacccgca ggtgcaaaaa
   121741 gcgttagcag acacggtctt acttcaggcc aacgtcacgg ccaacgacgc acaagatgtg
   121801 gcgctgttaa agcatcttaa tgtccttggc ctaccgacaa ttctcttttt tgacggacaa
   121861 ggccaggagc atccacaagc acgcgtcacg ggctttatgg atgctgaaac cttcagcgca
   121921 catttgcgcg atcgccaacc gtgaacgaca cttgcagtgg gaaagacgga ggaaaatagc
   121981 gtgcaacgtg aagatgtact gggagaagcc ctgaaattat tagaattaca agggattgcc
   122041 aacaccacgc tggagatggt tgctgaacgt gtggattatc cactggacga gctacgccgc
   122101 ttctggccag acaaagaggc aatcctctac gatgcgctgc gttatcttag ccaacagata
   122161 gatgtctggc gtcgtcagct gatgctggac gaaacgcaaa ccgccgaaca aaagttgctg
   122221 gcacgttatc aggcgttatc ggagtgcgtt aaaaacaacc gctatccggg ctgtctattt
   122281 atcgctgcct gtacgtttta tcccgatcct ggccacccta ttcatcaact ggccgatcag
   122341 caaaaaagcg cggcctacga tttcacccac gaactgttaa ccacgctgga agttgacgat
   122401 ccggcgatgg tagcaaagca gatggaactg gtgctggaag gctgtttaag ccgaatgctg
   122461 gtgaatcgta gccaggcgga tgtcgacact gcacatcggc tggcggaaga tatcctgcgc
   122521 ttcgcccgct gccgccaggg tggtgcattg acctgacaga aacacagaaa agaagcgatt
   122581 tgccgcaatc ttaagcagtt gaatcgcttt tactgaaatt aggttgacga gatgtgcaga
   122641 ttacggttta atgcgccccg ttgcccggat agctcagtcg gtagagcagg ggattgaaaa
   122701 tccccgtgtc cttggttcga ttccgagtcc gggcaccaaa ttcatatcaa cggacctcca
   122761 cggaggtccg tttttcgttt cagaacgcca caatttaagc gttctaccta caaatcaatt
   122821 ctaccgaact caaccagatt ctccccacat caaccccatt gtgtgggtat aattgcgggt
   122881 ataccccagt tcgacagaat ttgtaccctc tttaacgcat tcagaaggct gaactatggc
   122941 actgactgac gcaaaaatcc gggctgcaaa gcccactgac aaggcttata aactcactga
   123001 cggggccggc atgttcctgc tggttcatcc taatggttcc cgttactggc gtctccgtta
   123061 tcgtattctg ggtaaggaga aaaccctggc acttggtgtg tatccggaag tttctctctc
   123121 cgaagctcgt acaaaacggg atgaggcccg aaaactgatt tcagagggga ttgacccttg
   123181 cgaacagaaa agagttaaaa aagtagtccc tgatttacag ctctcttttg aacatattgc
   123241 acgacgctgg catgccagta ataaacaatg ggcacaatca cacagcgata aagtactcaa
   123301 aagcctcgag acacacgttt tcccctttat cggcaaccgg gatatcacaa cactcagtac
   123361 cccggacctg cttattcctg ttcgtgctgc agaagcaaaa caaatttatg aaatcgccag
   123421 tcgtctgcag caaagaatat ctgctgtaat gcgttacgcc gtacagtctg gcatcatcag
   123481 atataacccg gctctggata tggctggtgc attgaccacg gtaaaacgcc agcatcgccc
   123541 cgctcttgat ctttctcgcc tgcctgaact tttgtcgcgt attagcagtt acaaggggca
   123601 acctgtcacc cagcttgccg ttatgctgaa tttactggtt tttattcgtt ccagtgaact
   123661 cagatacgcc cggtggtctg aaattgatat tgacaatgcc atgtggacta ttccagccga
   123721 acgcgaacct ctgcccggcg taaaattctc acaccggggc tccaagatgc gaacaccaca
   123781 tcttgtgcca ctcagcaaac aggctgtagc catactgaca gaacttcaga catgggctgg
   123841 tgaaaatggt ctgatattta cgggtgcaca tgacccgcgt aaaccaatca gtgaaaatac
   123901 tgtaaataag gccctgaggg tgatggggta tgacacaacc caggaagtct gtggccatgg
   123961 attccgggcg
//
LOCUS       DAJOWB010000002        97994 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJOWB010000002 DAJOWB010000000
VERSION     DAJOWB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 97994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 97994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..97994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.002"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..159
                     /locus_tag="OHO85_000127"
     CDS             <1..159
                     /locus_tag="OHO85_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCQ2669561.1"
                     /translation="FIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKII
                     GGCGPTAQ"
     gene            403..1791
                     /gene="frlA"
                     /locus_tag="OHO85_000128"
     CDS             403..1791
                     /gene="frlA"
                     /locus_tag="OHO85_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCQ2669562.1"
                     /translation="MTANSPLQRIGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            1812..2834
                     /gene="frlB"
                     /locus_tag="OHO85_000129"
     CDS             1812..2834
                     /gene="frlB"
                     /locus_tag="OHO85_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCQ2669563.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2884..3714
                     /gene="frlC"
                     /locus_tag="OHO85_000130"
     CDS             2884..3714
                     /gene="frlC"
                     /locus_tag="OHO85_000130"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCQ2669564.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            3711..4496
                     /gene="frlD"
                     /locus_tag="OHO85_000131"
     CDS             3711..4496
                     /gene="frlD"
                     /locus_tag="OHO85_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCQ2669565.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            4596..5327
                     /gene="phoB"
                     /locus_tag="OHO85_000132"
     CDS             4596..5327
                     /gene="phoB"
                     /locus_tag="OHO85_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCQ2669566.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5479..6564)
                     /locus_tag="OHO85_000133"
     CDS             complement(5479..6564)
                     /locus_tag="OHO85_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCQ2669567.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDAQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(6576..7880)
                     /locus_tag="OHO85_000134"
     CDS             complement(6576..7880)
                     /locus_tag="OHO85_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCQ2669568.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFSLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(7892..8245)
                     /locus_tag="OHO85_000135"
     CDS             complement(7892..8245)
                     /locus_tag="OHO85_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCQ2669569.1"
                     /translation="MKKIGVAGLQREQIKKNIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(8256..9134)
                     /locus_tag="OHO85_000136"
     CDS             complement(8256..9134)
                     /locus_tag="OHO85_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCQ2669570.1"
                     /translation="MSIDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(9131..10357)
                     /locus_tag="OHO85_000137"
     CDS             complement(9131..10357)
                     /locus_tag="OHO85_000137"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCQ2669571.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIGRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVHTQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(10357..11520)
                     /locus_tag="OHO85_000138"
     CDS             complement(10357..11520)
                     /locus_tag="OHO85_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCQ2669572.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKIYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(11604..11966)
                     /locus_tag="OHO85_000139"
     CDS             complement(11604..11966)
                     /locus_tag="OHO85_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCQ2669573.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(11983..12888)
                     /locus_tag="OHO85_000140"
     CDS             complement(11983..12888)
                     /locus_tag="OHO85_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2669574.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQML
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(13157..14161)
                     /gene="trpS"
                     /locus_tag="OHO85_000141"
     CDS             complement(13157..14161)
                     /gene="trpS"
                     /locus_tag="OHO85_000141"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCQ2669575.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(14154..14912)
                     /gene="gph"
                     /locus_tag="OHO85_000142"
     CDS             complement(14154..14912)
                     /gene="gph"
                     /locus_tag="OHO85_000142"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCQ2669576.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(14905..15582)
                     /gene="rpe"
                     /locus_tag="OHO85_000143"
     CDS             complement(14905..15582)
                     /gene="rpe"
                     /locus_tag="OHO85_000143"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCQ2669577.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(15600..16436)
                     /gene="dam"
                     /locus_tag="OHO85_000144"
     CDS             complement(15600..16436)
                     /gene="dam"
                     /locus_tag="OHO85_000144"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCQ2669578.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPV
                     KK"
     gene            complement(16543..17829)
                     /gene="damX"
                     /locus_tag="OHO85_000145"
     CDS             complement(16543..17829)
                     /gene="damX"
                     /locus_tag="OHO85_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCQ2669579.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRETAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVRTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(17921..19009)
                     /gene="aroB"
                     /locus_tag="OHO85_000146"
     CDS             complement(17921..19009)
                     /gene="aroB"
                     /locus_tag="OHO85_000146"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCQ2669580.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(19066..19587)
                     /gene="aroK"
                     /locus_tag="OHO85_000147"
     CDS             complement(19066..19587)
                     /gene="aroK"
                     /locus_tag="OHO85_000147"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCQ2669581.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(19988..21226)
                     /gene="hofQ"
                     /locus_tag="OHO85_000148"
     CDS             complement(19988..21226)
                     /gene="hofQ"
                     /locus_tag="OHO85_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCQ2669582.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(21138..21542)
                     /gene="hofP"
                     /locus_tag="OHO85_000149"
     CDS             complement(21138..21542)
                     /gene="hofP"
                     /locus_tag="OHO85_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCQ2669583.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(21532..21972)
                     /gene="hofO"
                     /locus_tag="OHO85_000150"
     CDS             complement(21532..21972)
                     /gene="hofO"
                     /locus_tag="OHO85_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCQ2669584.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(21956..22495)
                     /gene="hofN"
                     /locus_tag="OHO85_000151"
     CDS             complement(21956..22495)
                     /gene="hofN"
                     /locus_tag="OHO85_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCQ2669585.1"
                     /translation="MNLPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLAHSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(22495..23274)
                     /gene="hofM"
                     /locus_tag="OHO85_000152"
     CDS             complement(22495..23274)
                     /gene="hofM"
                     /locus_tag="OHO85_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCQ2669586.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQQFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            23394..25946
                     /gene="mrcA"
                     /locus_tag="OHO85_000153"
     CDS             23394..25946
                     /gene="mrcA"
                     /locus_tag="OHO85_000153"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCQ2669587.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(26111..26671)
                     /gene="nudE"
                     /locus_tag="OHO85_000154"
     CDS             complement(26111..26671)
                     /gene="nudE"
                     /locus_tag="OHO85_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCQ2669588.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            26991..29126
                     /gene="igaA"
                     /locus_tag="OHO85_000155"
     CDS             26991..29126
                     /gene="igaA"
                     /locus_tag="OHO85_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCQ2669589.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTNIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            29191..29859
                     /gene="yrfG"
                     /locus_tag="OHO85_000156"
     CDS             29191..29859
                     /gene="yrfG"
                     /locus_tag="OHO85_000156"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCQ2669590.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            29870..30271
                     /gene="hslR"
                     /locus_tag="OHO85_000157"
     CDS             29870..30271
                     /gene="hslR"
                     /locus_tag="OHO85_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCQ2669591.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            30296..31174
                     /gene="hslO"
                     /locus_tag="OHO85_000158"
     CDS             30296..31174
                     /gene="hslO"
                     /locus_tag="OHO85_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCQ2669592.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(31309..33033)
                     /locus_tag="OHO85_000159"
     CDS             complement(31309..33033)
                     /locus_tag="OHO85_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCQ2669593.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            33412..35034
                     /gene="pckA"
                     /locus_tag="OHO85_000160"
     CDS             33412..35034
                     /gene="pckA"
                     /locus_tag="OHO85_000160"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCQ2669594.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(35110..36462)
                     /gene="envZ"
                     /locus_tag="OHO85_000161"
     CDS             complement(35110..36462)
                     /gene="envZ"
                     /locus_tag="OHO85_000161"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCQ2669595.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(36459..37178)
                     /gene="ompR"
                     /locus_tag="OHO85_000162"
     CDS             complement(36459..37178)
                     /gene="ompR"
                     /locus_tag="OHO85_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCQ2669596.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            37406..37882
                     /gene="greB"
                     /locus_tag="OHO85_000163"
     CDS             37406..37882
                     /gene="greB"
                     /locus_tag="OHO85_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCQ2669597.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            37979..40300
                     /locus_tag="OHO85_000164"
     CDS             37979..40300
                     /locus_tag="OHO85_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCQ2669598.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            40738..40965
                     /gene="feoA"
                     /locus_tag="OHO85_000165"
     CDS             40738..40965
                     /gene="feoA"
                     /locus_tag="OHO85_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCQ2669599.1"
                     /translation="MQYTPDTAWKITGFSHEISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            40982..43303
                     /gene="feoB"
                     /locus_tag="OHO85_000166"
     CDS             40982..43303
                     /gene="feoB"
                     /locus_tag="OHO85_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCQ2669600.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            43303..43539
                     /gene="feoC"
                     /locus_tag="OHO85_000167"
     CDS             43303..43539
                     /gene="feoC"
                     /locus_tag="OHO85_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCQ2669601.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            43742..44620
                     /gene="rpnA"
                     /locus_tag="OHO85_000168"
     CDS             43742..44620
                     /gene="rpnA"
                     /locus_tag="OHO85_000168"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCQ2669602.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(44649..45419)
                     /gene="bioH"
                     /locus_tag="OHO85_000169"
     CDS             complement(44649..45419)
                     /gene="bioH"
                     /locus_tag="OHO85_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCQ2669603.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            45457..46140
                     /gene="gntX"
                     /locus_tag="OHO85_000170"
     CDS             45457..46140
                     /gene="gntX"
                     /locus_tag="OHO85_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCQ2669604.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            46199..46774
                     /gene="nfuA"
                     /locus_tag="OHO85_000171"
     CDS             46199..46774
                     /gene="nfuA"
                     /locus_tag="OHO85_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCQ2669605.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            47135..48451
                     /gene="gntT"
                     /locus_tag="OHO85_000172"
     CDS             47135..48451
                     /gene="gntT"
                     /locus_tag="OHO85_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCQ2669606.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(48496..50580)
                     /gene="malQ"
                     /locus_tag="OHO85_000173"
     CDS             complement(48496..50580)
                     /gene="malQ"
                     /locus_tag="OHO85_000173"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCQ2669607.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(50590..52983)
                     /gene="malP"
                     /locus_tag="OHO85_000174"
     CDS             complement(50590..52983)
                     /gene="malP"
                     /locus_tag="OHO85_000174"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCQ2669608.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            53595..56300
                     /gene="malT"
                     /locus_tag="OHO85_000175"
     CDS             53595..56300
                     /gene="malT"
                     /locus_tag="OHO85_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCQ2669609.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(56343..57359)
                     /gene="rtcA"
                     /locus_tag="OHO85_000176"
     CDS             complement(56343..57359)
                     /gene="rtcA"
                     /locus_tag="OHO85_000176"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCQ2669610.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(57363..58589)
                     /gene="rtcB"
                     /locus_tag="OHO85_000177"
     CDS             complement(57363..58589)
                     /gene="rtcB"
                     /locus_tag="OHO85_000177"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCQ2669611.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTQKTVRQQQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            58778..60376
                     /gene="rtcR"
                     /locus_tag="OHO85_000178"
     CDS             58778..60376
                     /gene="rtcR"
                     /locus_tag="OHO85_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCQ2669612.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRTPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(60358..61116)
                     /locus_tag="OHO85_000179"
     CDS             complement(60358..61116)
                     /locus_tag="OHO85_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCQ2669613.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(61133..61963)
                     /gene="glpG"
                     /locus_tag="OHO85_000180"
     CDS             complement(61133..61963)
                     /gene="glpG"
                     /locus_tag="OHO85_000180"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCQ2669614.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(62008..62334)
                     /gene="glpE"
                     /locus_tag="OHO85_000181"
     CDS             complement(62008..62334)
                     /gene="glpE"
                     /locus_tag="OHO85_000181"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCQ2669615.1"
                     /translation="MDQFECINVAVAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            62524..64029
                     /gene="glpD"
                     /locus_tag="OHO85_000182"
     CDS             62524..64029
                     /gene="glpD"
                     /locus_tag="OHO85_000182"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCQ2669616.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(64083..64688)
                     /locus_tag="OHO85_000183"
     CDS             complement(64083..64688)
                     /locus_tag="OHO85_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2669617.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(64691..65458)
                     /locus_tag="OHO85_000184"
     CDS             complement(64691..65458)
                     /locus_tag="OHO85_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2669618.1"
                     /translation="MQPSPAMQALIEQIYHIFRRYPVPQKFVVCCEYCLSQQEQKVLR
                     STSLRAISYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWAFNNQSILKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(65461..66171)
                     /locus_tag="OHO85_000185"
     CDS             complement(65461..66171)
                     /locus_tag="OHO85_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2669619.1"
                     /translation="MELVNTLFASLVGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDLTVQFTAMLNPDERVIIAKRPLKKCESWIEDIYIFQVFKDAW
                     KFHNDNSLRGFRDKQAELKLYARYYTVENCAEESCRDCNSCIRPSFSLSRSAIIRLNV
                     ANARFVYQPFTRDQRARG"
     gene            complement(66186..67694)
                     /locus_tag="OHO85_000186"
     CDS             complement(66186..67694)
                     /locus_tag="OHO85_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCQ2669620.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFRQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLDFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNVDNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENMTSETQKLVEWFLTPQGQCLVEDVGYVPMYKTLH"
     gene            complement(67819..70266)
                     /gene="glgP"
                     /locus_tag="OHO85_000187"
     CDS             complement(67819..70266)
                     /gene="glgP"
                     /locus_tag="OHO85_000187"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCQ2669621.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(70285..71718)
                     /gene="glgA"
                     /locus_tag="OHO85_000188"
     CDS             complement(70285..71718)
                     /gene="glgA"
                     /locus_tag="OHO85_000188"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCQ2669622.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(71718..73013)
                     /gene="glgC"
                     /locus_tag="OHO85_000189"
     CDS             complement(71718..73013)
                     /gene="glgC"
                     /locus_tag="OHO85_000189"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCQ2669623.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(73031..75004)
                     /gene="glgX"
                     /locus_tag="OHO85_000190"
     CDS             complement(73031..75004)
                     /gene="glgX"
                     /locus_tag="OHO85_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCQ2669624.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(75001..77187)
                     /gene="glgB"
                     /locus_tag="OHO85_000191"
     CDS             complement(75001..77187)
                     /gene="glgB"
                     /locus_tag="OHO85_000191"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCQ2669625.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(77460..78563)
                     /gene="asd"
                     /locus_tag="OHO85_000192"
     CDS             complement(77460..78563)
                     /gene="asd"
                     /locus_tag="OHO85_000192"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCQ2669626.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            78756..79349
                     /gene="yhgN"
                     /locus_tag="OHO85_000193"
     CDS             78756..79349
                     /gene="yhgN"
                     /locus_tag="OHO85_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCQ2669627.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(79406..80746)
                     /gene="gntU"
                     /locus_tag="OHO85_000194"
     CDS             complement(79406..80746)
                     /gene="gntU"
                     /locus_tag="OHO85_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCQ2669628.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     FAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(80750..81277)
                     /gene="gntK"
                     /locus_tag="OHO85_000195"
     CDS             complement(80750..81277)
                     /gene="gntK"
                     /locus_tag="OHO85_000195"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCQ2669629.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(81416..82411)
                     /gene="gntR"
                     /locus_tag="OHO85_000196"
     CDS             complement(81416..82411)
                     /gene="gntR"
                     /locus_tag="OHO85_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCQ2669630.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(82635..83330)
                     /gene="yhhW"
                     /locus_tag="OHO85_000197"
     CDS             complement(82635..83330)
                     /gene="yhhW"
                     /locus_tag="OHO85_000197"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCQ2669631.1"
                     /translation="MIYFRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPNSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(83453..84490)
                     /locus_tag="OHO85_000198"
     CDS             complement(83453..84490)
                     /locus_tag="OHO85_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCQ2669632.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHLTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            84823..85311
                     /gene="yhhY"
                     /locus_tag="OHO85_000199"
     CDS             84823..85311
                     /gene="yhhY"
                     /locus_tag="OHO85_000199"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCQ2669633.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     MK"
     gene            complement(85372..87114)
                     /gene="ggt"
                     /locus_tag="OHO85_000200"
     CDS             complement(85372..87114)
                     /gene="ggt"
                     /locus_tag="OHO85_000200"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCQ2669634.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            87234..87674
                     /locus_tag="OHO85_000201"
     CDS             87234..87674
                     /locus_tag="OHO85_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCQ2669635.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(87661..88404)
                     /gene="ugpQ"
                     /locus_tag="OHO85_000202"
     CDS             complement(87661..88404)
                     /gene="ugpQ"
                     /locus_tag="OHO85_000202"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCQ2669636.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(88401..89471)
                     /gene="ugpC"
                     /locus_tag="OHO85_000203"
     CDS             complement(88401..89471)
                     /gene="ugpC"
                     /locus_tag="OHO85_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCQ2669637.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            complement(89473..90318)
                     /gene="ugpE"
                     /locus_tag="OHO85_000204"
     CDS             complement(89473..90318)
                     /gene="ugpE"
                     /locus_tag="OHO85_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCQ2669638.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(90315..91202)
                     /gene="ugpA"
                     /locus_tag="OHO85_000205"
     CDS             complement(90315..91202)
                     /gene="ugpA"
                     /locus_tag="OHO85_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCQ2669639.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(91300..92615)
                     /gene="ugpB"
                     /locus_tag="OHO85_000206"
                     /pseudo
     CDS             complement(91300..92615)
                     /gene="ugpB"
                     /locus_tag="OHO85_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            complement(93011..93724)
                     /gene="livF"
                     /locus_tag="OHO85_000207"
     CDS             complement(93011..93724)
                     /gene="livF"
                     /locus_tag="OHO85_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCQ2669640.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(93726..94493)
                     /gene="livG"
                     /locus_tag="OHO85_000208"
     CDS             complement(93726..94493)
                     /gene="livG"
                     /locus_tag="OHO85_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCQ2669641.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(94490..95767)
                     /gene="livM"
                     /locus_tag="OHO85_000209"
     CDS             complement(94490..95767)
                     /gene="livM"
                     /locus_tag="OHO85_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCQ2669642.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(95764..96690)
                     /gene="livH"
                     /locus_tag="OHO85_000210"
     CDS             complement(95764..96690)
                     /gene="livH"
                     /locus_tag="OHO85_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCQ2669643.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTVFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(96738..97847)
                     /gene="livK"
                     /locus_tag="OHO85_000211"
     CDS             complement(96738..97847)
                     /gene="livK"
                     /locus_tag="OHO85_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCQ2669644.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
BASE COUNT        23501 a        26963 c        24726 g        22804 t
ORIGIN      
        1 tttattaaag ttgcactggt aggtgcagta ctggctacgt taactgcatg tactggtcat
       61 attgaaaacc gtgataagaa ctgctcttac gactacctgc tgcacccggc aatttctatt
      121 tctaaaatca ttggcggttg cggtcctact gcacagtaag tctctgacag aaatcggcta
      181 acaccggttt aattgcccga cgccctctgg attttccgag ggcgtatttg tttctatgtc
      241 cttcttgccc ccgcccgtaa aaatattttc actttaaatt caatttgata aatacatcca
      301 ttagccacag cgcgatcctg tgcgaaattt tgtgatcttc ctccacatta cataacatca
      361 tatgttgtta tattcatcat gcattgtcat gttacctttt aaatgactgc aaactctccc
      421 ctacaacgta ttggacaaga aaaaggtatc gctatgggaa gccaggaact ccaacgcaag
      481 ctcggatttt gggccgttct tgcaatcgcc gtcgggacaa ccgtcggctc cggtattttt
      541 gtatctgtgg gtgaagtggc aaaagcagcg ggcacgccgt ggcttacggt gctcgcgttt
      601 gtcattggcg ggttaattgt gatcccgcaa atgtgcgtct atgcggaact atccaccgct
      661 tatccggaaa atggcgcaga ttatgtttat ctgaaaaatg ccggaagccg accgctggct
      721 ttcctctccg gctgggccag cttctgggcc aacgatgcgc cgtcattgtc gattatggcg
      781 ctggcgattg tcagcaatct tggcttttta acgcctatcg atccgttgct cggtaaattt
      841 atcgccgccg gattaattat cgcctttatg ttgctacacc tgcgctccgt tgaaggcggc
      901 gcagcgtttc agacgctaat taccatcgcc aaaattatcc cgttcactat cgtcattggc
      961 cttgggatct tctggtttaa agcggagaat tttgccgccc ctaccaccac tgcgattggc
     1021 gcaacgggca gctttatggc gctgctggcg gggatctctg ccaccagttg gtcgtatacc
     1081 ggcatggcct ctatctgtta tatgaccggc gaaattaaaa accccggaaa aaccatgcca
     1141 cgagcgctga ttggttcctg tctgctggtt ctggtgctct acaccctgct ggcgctggtg
     1201 atttccggcc tgatgccctt cgacaaactc gccaattctg aaacgccgat ttccgacgcc
     1261 ctgacctgga tccccgcact cggcagcacc gctgggatct ttgttgccat cacggcgatg
     1321 atcgtcattc ttggttcgct ttccagctgc gtgatgtacc agccgcggct ggaatacgcg
     1381 atggcgaaag acaacctgtt ctttaaatgc ttcggccatg tgcatccgaa atacaacacg
     1441 ccggatgtct ccatcatcct gcaaggggcg ctggggatct tcttcatctt cgtttccgat
     1501 ctcaccagcc tgctgggtta tttcaccctg gtgatgtgtt tcaaaaatac cctcaccttc
     1561 ggctccatca tctggtgtcg taaacgcgac gattacaaac cgctgtggcg tactccggct
     1621 ttcgggctga tgaccaccct cgccattgcg tcaagcctca ttctggtcgc ctcaaccttt
     1681 gtctgggcac cgattcccgg ccttatctgc gccgtcatcg ttattgctac tggtctgcct
     1741 gcttacgcct tctgggcgaa gcgtagccgc cagctcaacg ctttgtcgta atgttatctg
     1801 gagaaaataa aatgttggat attgataaaa gcaccgtgga ctttctggtc accgaaaata
     1861 tggttcagga agtggaaaaa gttctcagcc atgacgttcc gctggtgcac gccatcgtgg
     1921 aagagatggt gaagcgcgac attgatcgta tttatttcgt tgcctgcggc tcgccactca
     1981 acgcggcgca aacggcgaaa catctggcgg atcgcttttc cgatcttcag gtctacgcca
     2041 tttccggctg ggagttctgc gataacaccc cgtatcgcct cgacgatcgt tgcgcggtga
     2101 ttggcgtttc tgactacggt aaaacggaag aggtaatcaa agcgctggag ctgggccggg
     2161 cctgcggcgc actcactgcg gcgttcacca aacgcgcgga cagcccgatt acctcggcgg
     2221 cggaatttag cattgattat caggccgact gtatctggga aattcacctg ctgctctgct
     2281 acagcgtggt gctggagatg atcacccgcc tcgcgccgaa cgcggaaatc ggcaagatca
     2341 aaaacgatct caagcagttg ccgaatgcgc tcggtcatct ggtacgcacc tgggaagaaa
     2401 aaggccgcca gcttggtgaa ctggccagcc agtggccgat gatttatacc gttgctgcgg
     2461 gtccgctgcg tccgctgggt tacaaagaag gcattgtaac gctgatggaa tttacctgga
     2521 cgcacggctg cgtgattgag agcggagagt tccgccatgg cccgctggag attgtcgaac
     2581 cgggcgttcc gttcctgttc ctgctcggca atgatgaaag tcgccacacc accgaacgcg
     2641 ccattaactt tgttaaacag cgtactgaca acgtgatcgt catcgattac gccgaaattt
     2701 cgcaagggct gcacccgtgg ctggcaccgt tcctgatgtt cgtgccaatg gagtggctct
     2761 gctactacct gtctatttac aaagatcaca acccggatga acgccgctat tacggtggtc
     2821 tggtggaata ttaatccctc tcccggcccg gcaacgggcc gaatttacgc aaggagttac
     2881 ccgatgaaaa caggtatgtt tacctgcggc caccagcggc tgcctattga acacgcattt
     2941 cgtgatgcaa gcgagctggg ttacgacggc atcgaaattt ggggcggtcg cccgcacgcg
     3001 ttcgcgccgg acttaaaagc gggcggcatc aaacaaatca aggcgctggc gcagacgtat
     3061 cagatgccga ttatcggcta tacgccagaa accaacggct atccgtataa catgatgctg
     3121 ggcgatgaac atatgcgtcg cgaaagcctc gacatgatca agctggcgat ggatatggca
     3181 aaagagatga acgcgggtta tacgctgatt tccgcggccc acgcgggcta tctcacgcca
     3241 cctaatgtta tctggggacg gctggcagag aacctaagcg aactgtgtga gtacgcggaa
     3301 aacatcggca tggatctaat cctcgaaccg ttaacgccgt atgaatcgaa cgtcgtgtgt
     3361 aatgctaatg atgtgcttca tgcgctggcg ctggtgcctt cgccgcgcct gttcagcatg
     3421 gtcgacattt gcgcgccgta tgtccaggcg gaaccggtga tgagttattt cgacaaactg
     3481 ggcgataaat tacgtcatct gcatattgtc gacagcgacg gggccagcga cacgcattac
     3541 attcctggag aaggcaaaat gccgctgagg gaactgatgc gcgatattat cgaccggggc
     3601 tatgagggtt actgtacggt ggagctggtg acgatgtata tgaacgagcc cagactctat
     3661 gcccgccagg cgctggaacg ctttcgcgcg ctgctgccgg aggatgagag atgaaaaccc
     3721 tggcgacaat cggcgataac tgcgtcgata tctacccgca actgaataaa gcgttttctg
     3781 gcggtaatgc ggtcaatgtg gcggtgtact gcactcgcta cggcatacag ccgggatgca
     3841 ttacctgggt gggtgacgat gactacggca caaagctgaa gcaggatctc gcccgcatgg
     3901 gcgtcgatat cagccatgtc catacgaaac acggcgttac cgcacaaact caggtggaac
     3961 tgcacgacaa tgatcgcgtt tttggcgact acaccgaagg cgtgatggcc gactttgccc
     4021 tgagtgaaga ggattacgcc tggctggcgc agtatgacat tgtgcacgcg gcaatctggg
     4081 gacatgcgga agacgcattc ccacagctgc acgctgcggg caaacttacc gctttcgact
     4141 tctccgacaa gtgggacagc ccgctctggc agacactggt gccgcatctc gattttgcct
     4201 ttgcctccgc accgcaagaa gacgaaacgc tgcgtctgaa gatgaaagcg attgttgccc
     4261 gtggcgcagg aacagtgatt gtcacgctgg gtgaaaacgg cagcattgcc tgggatggcg
     4321 cgcagttctg gcgtcaggct cctgaaccgg tgacggttat cgacaccatg ggtgccggag
     4381 attcgttcat tgccggattc ctttgcggct ggtctgcggg gatgacatta ccgcaggcga
     4441 tggcgcaggg aacggcgtgc gcggcgaaaa ccattcagta ccacggtgcc tggtaggtat
     4501 aacgttggcg tgagcatctt cacgccaacg tgctgttact tgccggaaaa cgaccctata
     4561 atccgagtaa ttcattcttt atttcagggt cgattatgtc agctacggac cgctactctc
     4621 atcaactcct ctacgctacc gtccgccagc gactgctgga tgatatcgcg cagggggttt
     4681 accaggccgg gcaacagatc cctaccgaaa acgagctttg tacacaatat aacgtcagcc
     4741 gcattaccat tcgcaaagcc atcagcgact tagtggcaga cggcgtactg atccgctggc
     4801 agggaaaagg cacctttgta caaagccaga aagttgaaaa cgccctgctt actgtcagtg
     4861 gttttaccga ttttggcgtc tcacaaggca aggcgacgaa agagaaagtg atcgaacagg
     4921 aacgggtcag cgccgcgccg ttttgcgaaa agctgaacat ccccggaaac agcgaagtgt
     4981 tccatctctg ccgggtgatg tatctcgata aagagccgct gtttattgat agttcatgga
     5041 tcccgctgtc gcgttatcct gactttgatg agatttacgt cgaaggaagc tccacctatc
     5101 agttatttca ggagcgtttt gacacgcgag tggtcagcga caaaaagacc atcgatatct
     5161 ttgccgccac ccgcccgcag gcaaaatggc tgaaatgcga actgggcgaa ccgttgtttc
     5221 gcatcagcaa aatcgccttt gatcagaatg acaaaccggt gcacgtctcc gaactcttct
     5281 gccgcgccaa tcgcatcacc ttaactattg ataataaaag acattaaccg taggccggat
     5341 aagatgcgcc agcatcgcat ccggcgatgc tggcgcgttg aattttacat ctcgtacgtt
     5401 cccctcaccc taaccctctc cccaaagggg cgaggggacc gttcagtacg gaatttagct
     5461 tgacggtgct atccagcatt aaatactggc aatactctca cgcaaaatcc gcagcaccgt
     5521 ctcttcaccg ctgcgattcg ggttaatgcg aatcgcacaa tgttctgatt gtggattcgc
     5581 ctggcgaaac gttccggaaa ggcgataaaa gagcggcggg atttcatatt tcgactctgc
     5641 acccaccggg taaggcaagg caccgcgctt ttgcgcctct tccagcactc tggcggcaat
     5701 cggctgatga aactcgacaa tcaacacctt cgactgcgca ttagcaatca ccgcgctttt
     5761 cacttccggc accgcaccac cgttaagcaa agccagcaac cgttcagata cccctgcctg
     5821 caccgcgtgc atcactggcg caaacaccag accacgcaat acttccagcg cctgtgcgcc
     5881 ctggatctgg ctaccgccgg agtaaagcgt ggcgcgaata cggttgataa catcagcatc
     5941 gccgaccact gcaccaacac cctctggccc aaatagcttg aagcaggaaa atgtcgagac
     6001 attcgcgccg cattcacagc cgattcgcgc caccttcatc accgcatagt tgtcatcggt
     6061 taacgctgga acacctgccg cgcgcaacgt tgccagcaca tctgccagca cgtagctgtc
     6121 ctgcggctgc tggcgcgtat gctgcaccag cgccgcatcc ggttgttgcg cgtcgacgac
     6181 ctgcttcagt gccgacaggt cattgaaatc aacagtaata agcgtcagcc ccatctgctc
     6241 aataataacc cgtgtcgtcg ggtaaacagg cgcgtcatgc accagaagac gctgccccgg
     6301 tttgagcaaa gccgccagcc cggcgcgaat cgcgccagtc cccgcgccct gcaccagcgc
     6361 cgcagcctgt gcgtgaaatg catcagccag cacctgttcc acacgctggg taacgcgcgg
     6421 ttgattcagt cctggcgtta agcctaaatc accgccagta agaaactcgc tgccggggaa
     6481 atggcgacaa atgctatcca ccagcgcaaa ctgcttttgc tgcgcctcaa taatcgtcag
     6541 gctttgcaga ggaaacgtct tcataggtgc cttccttaag ccggaacgaa caggccaagc
     6601 cagtaaagaa cattcagcag aataccggtg atcattactg ccacgaccgg tgccgccatt
     6661 ttctgtaccg gacggcccag ggattcgttg aggaagtaaa tggcaaccgc gatagagaat
     6721 ccggtataac ccgccatctt aattgctgcg aaaatcgaac cgaccagcag cgccacttcc
     6781 atcagcatat tcatggcgtt acggatgtta tccgacgcat tacgcaccga cgggtagcgt
     6841 cccagccatt tgccgatcga acgaagcagc aagacttccg ccgaaataac cactgcgcct
     6901 aataccgctg caaccatcgg attcggcgag agatagccca ccgcataaac aaaggtaaag
     6961 cccgcaactg catacacacc cgttgctaag gcggtggtgg caatcaacgg tacaaaaccc
     7021 agcccgcgca taaattctgc cagtgccgcc tggttgatca gcgtttgcga ttgttccggc
     7081 gttacgcctg cggagtacgc tttctccaga gtgaagatcg acacttcact gccggcgaaa
     7141 atcttcatgc tggcaacggc ggcaatcaat gctcccacga tggcgatata gggtaagttt
     7201 ttgataatcc gcgacgtgcg ttcttcaaac accgaaagcc cgctggcatc aatatcattt
     7261 tcatcacgat gacgcaggtc gtgagttatc gcgatcccca gcagcatcac catgccaata
     7321 aagatttcga tggattcagg gttaagatgt gggaaatagc gcacgactac cacacgggtc
     7381 atcagaacaa ccacggcggc gaccagactt tgcttccagc caaactggta gaaaatcgcc
     7441 accagcggga acagtgcaaa agcagaaacc accggcgagc ttaattcccc gaggctacct
     7501 aatacatcca ccggcagcgc ggtcagcagc tggtttactg gcaacaggca agtaaggatc
     7561 aacacgcccc agatagcgcc aagaccaaac gccatcaggc tgtttatcgc cagtacgccg
     7621 aggatatcgg taggaagaaa gagtaaccat gcgttgagca gcccggtttt cagggaaaaa
     7681 gagatcccca ccgaggccac aaaaccaatg ctcagaccaa aagcgatact ccccgcctcg
     7741 cgacgattca tatagccttc aatcagttgc ggcaggatcg ggcggatgcc gtcatgaaaa
     7801 acagccgccg agcgatgcgc cagaagcgat gtcatacccg tcaggcacgc caccacgata
     7861 atctgaatat acagatccat agtcgtgcct tttatttcag atggttaatc agcatcggaa
     7921 tcgcgtgttc gacgtgctca acggaaaggc caaacgccac tttcccttca gcgatcattt
     7981 tggcgatatg ctcgtctttc gctttaatgc ctggtttggc aatggtgcaa cttttgttat
     8041 agccaatcac cgcgatggca atcgacaatg ccgcgcccgc accggtatta cacgcaccga
     8101 tgtagtaatc cagttgcccg gatttcactt tcattgccgc ttccatgtcg ttgtgaatga
     8161 aaacttcaaa actgccagga gccgtcgctt caatgttttt tttaatctgc tcacgctgta
     8221 agcctgcaac gccaatcttt ttcatgagtc tgtccttatt ggaaaaattg agagggattt
     8281 tcacgtaaca tcacatccac atcggcctga ctgaatcctg actggcgcaa ttgcggaata
     8341 aaggtggtca ataaatagtc atagccataa ccaccattgg cttttaaatg ggagcggcgc
     8401 gtaatatcca tcgacagcat gacgcggttc agcaacccac ggtcacgtag cgcgtggagc
     8461 atcgcaatgc gcttttcgtc cgggtagtaa ctgttcttac caatggtgtc gaactgcacg
     8521 tacgcgccga gatcgatcat cttcaaaatg ttgtcgagat tgtctttcag atcgcagtga
     8581 ccaacggtga cgcgcgaaag atcaactccg tgggcttgca acagagccag ttgttccagc
     8641 cccatggtgc tgaacgaagt atgcgtggag atcgggcgtc cggtctggtt atgcgccagc
     8701 gcagcggcaa taaacacctt ctcttccagc ggcgtaatct tcccttcgct agagccgatc
     8761 tccgcgataa tcccggcttt caggtcggtg ccatcgatac cctgttcaat ttcatcgacc
     8821 atctcctgcg ccagttcctg cacgctgcgg gtcgccacat gctccgggaa aaaagcgtcc
     8881 tggtaataac cggtgcaggc caccacgttg atccccgtct cgcgcattac atcgagcata
     8941 aattgcgcat tgcgccccat gtaacggttg gtcatctcaa tcacattacg cacgccccgg
     9001 gtcatcaggt cgttcatctc ctggcaaatg aacgcatact gatcaaggcg gcagtccacg
     9061 ttgtttttaa agccggagag atcaatatgc agatgctcat gggccagggt gtaacccgtc
     9121 ggatcaatac tcatagggtg tctcctgcaa accggaggga ggaaagaaaa gagcgaccat
     9181 tttgtggcgg tggcgcacgg aaaaattcac acacggtagc ccccacatca gaaagcgtgg
     9241 tgcgtacacc gagttgcgtg tggaccagcc cttgctgata aaccagcact ggcaccactt
     9301 cacgggtatg gtggctgtga ccaatggtcg gatcgttacc gtgatccgcc atcacgacca
     9361 ggcaatcatc tggctgcatc gcctcaacaa gccgggcaag gttacggtca acgacctgca
     9421 aacgttcggc ataacgtgcg acgtcttctg catgaccagc gaggtcggtt tcctggatat
     9481 tgatgcaaat aaacgccgtc ggatgcgtgt taaattcgtc gagggtgata tccataatcc
     9541 gctggctatc caccaggttt tgccagctca cgccataagg attgctgacg atatctgcca
     9601 ccttacctac cagcacggtt ggcacgcccg cctcatacag tttttgcggc acctgcactt
     9661 tttcatcaac accgtagccc atatgcacga cctggaagcc gttgtcataa gcgccagaac
     9721 gcggcgcatt aataccaata aagcgccctt ctttgctttc tgcggcatcg agaatacgct
     9781 gactgtcggt taacaagccg ccaaatgtaa tgacccgacc gacctgtacc tgctcacgca
     9841 cgatacgacc aattttgatc gcgtcgtcaa aagagatcac agagagattg gcggtaatgt
     9901 tatagacctg gcctaaatcc gcctcgagat tatcgccaat cgcaaccgcc tgattgaccc
     9961 acagaaattg cagatcatcg ccacggcgct ccacctgcca gccagcggaa actaatgcct
    10021 gctcaacacg gccaatcaca tcgcgaaagg gcatccgcag cggcggtaac gggcgcgtgc
    10081 ctaaaatttc ctgatgcccc ataaaggtat cgccaccttc atgttgcagc tctgccacgc
    10141 cccaggttgc ggaatctgac ggctgcatat cgcctggcgc ataacccaat gcgttgatta
    10201 gccccagctt ctccagcgtt ggtagctgca aatgcggcaa ctggctcagg atgtgaccac
    10261 aggtattcgc cccggcatct tgcggacgca ccagcgtgac atctttcatt gcccctacgc
    10321 caaagctatc aatcactaac accacaaatc gcgccattac gcccccagag agtttccaag
    10381 actgtcataa cgaccaacga ttttcggttc gccacggtga ataccggaca ccagcaccac
    10441 atcgctacgg gtgacaaaaa tctgcgtgcg gaaacagagc accactgcac tgctcaccgg
    10501 atactcgcct gccagcggca gggtgtagtc gatactgctg tcatccacag ttttgagatt
    10561 ggtttcagta atcttttgat tttctggcgt aaacaccagc gcatgttgcg catgaccacg
    10621 acggtagtaa ccgccgccgt agcagtagct gtcgccacgg aaatgatggg agatttcact
    10681 taaccagagc atcgcgatac gttcaggctg atcgccctgc tggtttgccg gaatagtgcc
    10741 cgtcagcgca tgaccgggtt cggcatgagt cacaccgtat tccgccagca atggcagcga
    10801 agtgcagctg gtcgctgaag gcgcgttcag ttgctcaagt gcaataccag atttcgccag
    10861 ttgatcccgt gcctgtatca gcgtgtgaag attcggtgtc ggcaaaactt ttccggcagc
    10921 ctcatcccaa agcaggcaag ggaaatgggt aagtccggct aaatgcagcc ccggcagttt
    10981 cttaatttca gcaaccacct cgtgcagcga atgctgtaca aaaccgctct cctgccccgg
    11041 ataaagaaaa tcatcgtcgc tataaatttt gagcagcaca aactgcacac gtccggcctt
    11101 caccgctgcc gcagaaactt cccgcgcttt gtcgagagta aacacggtga tgacgtcggt
    11161 gccctgttca acggcgtcag caacctgatg acaagggatt tgtaccagat gcccctgatg
    11221 cgccaccgac aaaccagcgc ggcgcatgac tcgcgcctct ttgtaatcca ccgccacaat
    11281 gccgctgtaa cctaatgcca gcaatttttc cgccagccat gggttacggc caaactgctt
    11341 ggtcatcaga tacagttcaa tgccgtaaag ccgcgccgtc tcaatcagtc gcttaccgtt
    11401 ttccagtacc tgatccacgt cgatcaccca gctgtccggg gcgatcttgc cctgctgcca
    11461 caaacttagt gcggcggaaa tcagcgccgg gttctggcgt ttcaatgctt ctacaaacat
    11521 cttgttgcct cgtttctgtt ccttgaatta aatattcatt tttttgaata tttaggttgt
    11581 gcgacgtgga tttgtcggga gcttcaaacc tcttcgttag cagccatcca taatccataa
    11641 aggttggcca gcaaatagcc ttcttcacac gggttaactt ccagtgcgaa ttccttcaac
    11701 aacacctgat gcaattgcac aacggcttgc cagtggctgg attgcgccag ctctgccagc
    11761 aattcgttat ccagcggctc tatttcttca ccacggcgac tgcgcatcag tgcactcgcc
    11821 atatgtgtca tcgccatcgt tccttgctca ctgcgcaccg gcagatggca ctctgtttcc
    11881 agtacgttga cgacctgcat catgcctttg cagatgtcct tatcaataac gcctgcctcg
    11941 caaagcaggt tgagtctggt ttccatatca gatgcctttc gtttaataac tcggttcctg
    12001 ttccccactc actacccgtt gttgatgggc gagcagggcg tgtttatcta caacggcacg
    12061 cagcatttgt tgcattgggt aatcatcgac tcgcgtcagg accacggctt cggtggcctg
    12121 taaaaatcgc ggatcgtctg tgagcggcgt cgcctctaat cccagcatgg tcagttcgtt
    12181 ttccgccacc acgttccaga tcaccgcatc gacatcgcct ttaacaatgc gttgtaaact
    12241 ctcgtgataa gagagatcga ctcgttccac atcactaccg ccaaaaaaaa catcggtcat
    12301 gattttctga tccgccgaac ggttatccag ccccacgcgc ttcacgtttg cggactcgcc
    12361 tttacggcaa atcaactggt gctcgccaac gtaggtgtgc ggccccaact ccagcgcgag
    12421 gcataaccct ttttgcgtga gataactttc cgccgccagt cgggaaacca ccgccatgtc
    12481 atacacgccg ttaagtaaac actccacgcg aatatccgcg ccgcgcatat gcgcatagta
    12541 aaaaggaatg ccatcaaact gagctttcag cccgctcgct aaaccttcgt acaagcgggt
    12601 atagggcaaa ggcatcgcac ataccacgtt gttgatatcc acatgactca gcaatgcttt
    12661 gttatccatc tcgaccagat aactgccatt gcgcccacgg cgttcaatcc gtatcgctcc
    12721 gcttgattcc agcgttttca acgcggcctg cgtcaggcca accgatgaac ggcattcagt
    12781 tgccagctca tctatggttt tcaacctatt accgcatttt tcacccaaca aataacgggc
    12841 cagcgtcgtg ataacgacgc cttctttttt gataaacgtt cgacgcataa tatattttca
    12901 ataaaatgaa tgtttatatc ttcattaaaa tgaagataag tggcaactgt gagatgtgga
    12961 aacggcgagg cgcttcacat tttttcggat tattgcaaat gaggataaaa aaaccgggtt
    13021 tccccggttt cagagtgatg acgaagtgat ttattcgaca atgcgagcaa agggtaattc
    13081 ccactggcgc ttgcctgctc ccctcaccct aaccctctcc ccaaaggggc gaggggaccg
    13141 tccgagcgtt ttgatattaa ggcttcgcca caaaaccaat cgcttcgtac accgctttca
    13201 gcgtacggga agcgtgtaca ctggcttttt ctgcgccgtc tttcatcacc tgttgcagga
    13261 aggcttcatc gttgcggaaa cggtgatagc gttcctgcaa ttcagtcagc ataccggaaa
    13321 cggcatcagc cacttcacct ttcagatgac catacatctt gccttcgaac tgtttttcca
    13381 gttctgggat gctctggccc gttaccgcag aaaggatatc cagcaggttg gaaacgcccg
    13441 ctttgttctg cacatcgtag cgaactaccg gcggctcgtc ggagtcagtg accgcgcgtt
    13501 tgattttctt cactaccgat ttcggatctt ccagcaggcc gataacgtta ttgcggttat
    13561 cgtcagactt ggacatcttc ttggtcggct ccagcagcga cattacgcgc gcgccagatt
    13621 tcggaataaa cggctccggc accttaaaga tctcgccata cagcgcgttg aaacgctggg
    13681 cgatatcgcg actcagttcc aggtgctgtt tctggtcttc acccaccggt accagattag
    13741 tttgatacag caggatgtcc gcagccatca gcaccggata gtcaaacaga ccagcgttga
    13801 tgttctcggc ataacgcgca gatttatctt taaactgggt catgcggctc agttcgccga
    13861 agtaggtata gcagttcagt gcccagccta actgcgcatg ttctggtacg tgggactgaa
    13921 caaaaatggt gcttttctca ggatcgatac cacaagccag atacaaggcc agcgtatcca
    13981 gcgtcgcttt acgcagcttc tgtgcatcct ggcgcacggt gatcgcgtgt tggtcaacga
    14041 tacagtaaat gcaatggtag tcatcctgca tgtttaccca ctgacgcagc gcacccatgt
    14101 agttaccaat ggtcaattca cctgagggct gtgcgccact aaaaacgatg ggcttagtca
    14161 tttttcgatt cctgattttc gctatgcgga agccctaatg cgggcagaag gtcatttata
    14221 gactgataaa ttacatcagg ctggctgaga tcgatagcct cgccgtagtt atatccgtag
    14281 gttaagccaa ctgatgggca acctgccgct tttgccgcct gaatatcatt gcgtgagtcg
    14341 ccgacaaaca gcatctgttg tggggcaatt cccatccgct cagccaccag taacagcggg
    14401 tccggatgcg gttttttgtt ttgcacatca tcgccgccga taaccacgct aaagtatttg
    14461 gcaatatcta aggcttcgag cagcggcgcg acgaacggcg tcggtttgtt ggtgaccagg
    14521 cctagcggca ggcctttagc ctgcaacgcg cccaacgtat cggcaacgtg cgggaacaaa
    14581 aacgttccct cttcggcaac ctcgctatag tagcgatcga acagtttacg cagaatacgt
    14641 acctgttctt ctgccggaat gtcgtcatca acgggcggtt tacccattgt tttacgctga
    14701 gtcgcacgtt cctgacgcgc ccaggtcaat gcgcgctcca tcagaacatc tgcgccgtta
    14761 ccaatccagg taataacgcg ttcttcacct gcgatgggca actccagcgc atacagcgcc
    14821 atatctaccg cagcagcaag accgggagca ctgtcgacca gcgtaccatc aagatcaaaa
    14881 gcgacgccgc gaatatcttc aaacttattc atgacttacc tttgccagtt cactgcgcat
    14941 ttcatcaatg acttttttgt agtctggctg gtcgaagatt gccgaaccgg cgacgaacat
    15001 atccgcgccc gccgcagcga tttcgccaat gttgttcacc ttcacgccac cgtctacttc
    15061 cagacgaatg tcaaagccag actcgtcgat acgacgacgt acttcgcgca gtttatccag
    15121 tgtttgagga atgaacgact gaccgccgaa accaggattg acggacatca gcaggatcac
    15181 atccagctta tccatcacgt aatccagata gctcagcggt gtcgccgggt taaatactag
    15241 tcccgcttta cagccatttt ctttaatcag ttgcagcgtg cggtcaacat gctcggaggc
    15301 ttctggatga aaggtaatga tgctggcacc agcggcagcg aaatcaggca caatgcgatc
    15361 gacaggtttc accatcaggt gtacgtcgat aggggcggta atgccgtagt tacgcaagga
    15421 tttcagcacc attggcccaa tcgtcagatt gggaacatag tggttatcca tgacgtcaaa
    15481 atgcacgaca tcagcgccag ctgccagggc ttttgcggta tcttcaccca ggcgggcaaa
    15541 atcagccgac agaattgagg gggcaatcaa atactgtttc atccgcttct ccttgagaat
    15601 tattttttca cgggtgaaac gactcctggt ttgtacaaag ccagcagttc gtccaccttt
    15661 ttacgtgtgc cgccgttgct gcttatactg cgtcgaactt tgacgacatg caattttgcg
    15721 cgctgatacc actcacgcgt taacatcgta tcgtgattgg agatcagcac aggaatatgg
    15781 cgatcaacca gaccttcggc gatctccgcc agatgcgctt gttgttcaag cgtaaaactg
    15841 tttgtgtgat acgccgtaaa gttggcggtc gcagacagcg gtgcataagg cggatcgcaa
    15901 tagacgacgg atgcatcatc tgcgcgcgcc atgctatcgg cgtaagactc acaatagaaa
    15961 aaggcattct gcgctttttc agcgaagtga tacaactctg cttccgggaa atagggtttt
    16021 ttgtagcggc caaacggcac gttaaactca ccgcgcagat tgtaacgaca caggccgttg
    16081 taaccgtagc ggttcaaata taaaaacagt accgcccgac ggaacggatc ctggcttttg
    16141 ttgaactctt cgcggaactg atagtaaacc tcggcgcaat ttgtttcggg aacaaacagc
    16201 tcgcgtgcgg cctgtacgta ctcatcagta cgcatcttca caatgttata gagactgatc
    16261 aggtcgctat tgatatcggc aaggatataa cgagaaaagt cggtgttgag aaacaccgac
    16321 ccggcaccta caaaaggctc aaccagacat tcgcccttgg gcaaatgccg tttaatatca
    16381 tcaagcaggg gatacttgcc ccctgcccac ttcaaaaaag cgcgattttt cttcatgctg
    16441 actaactaat tacaccttct ccggctgtgg agaaagctcc gacagcatcc agcgcttcag
    16501 gcaactcccg caacgataaa ccattgcggg agataacctt gattacttca gatcggcctg
    16561 tacctgacgc agcggtttcg cccacgggtt tttggcctga acatctgctg gcaatgtaga
    16621 taccgctttt ttcgcctctt ctttcgaagc gtacacgcca gaaaccagga cataccacgg
    16681 ctgaccatta cgcgtcgttt catagacaac gtagtttttc agattctctt tcttcgccca
    16741 accgttcagg ttgtcgtagt tagaggaact gctcagctgc agagtgtaat ggctggacgg
    16801 tgccgatttc aacgaaccaa cattacctgc ggtcttccct ccagcggctg gtgttgccgt
    16861 ggtttgcgcc ggggatgccg tccgtactgg agccgtagtc gccgtctctt ttggtgctgg
    16921 cgtagaagtc gcagccggtg ccttcgtgct cgccacaggg gcagctggtt cagtacgctt
    16981 cggcgtctgt gctaccggct tcggctccgt tttcacggtt gcttgcggtt ttttcggttc
    17041 aatcactgcc tgctggcgag ctggacgcgt agtggccgga cgttcagcgg tttgcgtttt
    17101 cgccgtttca cgggatgcat tgccattgcg aacaggcgcg acagtcgcgg gttcggtcgg
    17161 caatgtggaa ttgaccgcca cattgttcaa ctgttgctga ttttgtggct gggtcagcgc
    17221 attgttcagg tcaccctgca cttcaacacg ttgttgacca tccgttgccg ccggggtttg
    17281 cccttgagtc ggcgtagaag agatcggtgg cagagaaaca tcctgctgag tattttccgc
    17341 agacgtggtt cccggcgctg gctgcacgcc attcgcctga tcggtcgcat tgccagcaag
    17401 atcaatactc ttctcgccag acgcggtttg atcgctggaa gtggtcgagg gggcttttag
    17461 cgcagaaccg ataccgatga tcaacagcag tagaaccaga atgccgacgc ccatcatcat
    17521 atactgacga gaagcgggtt tactggctgc ttttttgcgc ttacgcggac gacgctctac
    17581 gcgctcttca tccacggttt catcttcgga ttcgtcaatt tcttcttcga tttccggttc
    17641 ctcattgcgc tctttttgcg cacgagtcgg acggcgatcg tcagtgtcat caagttcaat
    17701 atcatcaaaa ttgatctgcg gttcgccacg ttcagtacgc tcagaacgtt cagaagattg
    17761 acgagaacga ccagtacgac gatcgctggg atcgggtttc agctcgtctt ctggtttgaa
    17821 ttcatccatt taacacccca ctaaaaggtt aatgcttacc acgttgcaat taacctgaag
    17881 ctaatagacc gcttgataag cggcctgacc tttcttgttg ttacgctgat tgacaatcgg
    17941 caatggcgtt aagaacaagc tcgtgcgaaa cgccgctgcg aacttcactc ttaccaattg
    18001 ccaacggaag aattaagcgc atctctcccg caaggacttt cttgtcacgc agcatatgcg
    18061 gtaaatacgc ctgcgcggac atttcgcgcg gcccattgac cggtaaccca gcccgcgtga
    18121 gcagggttat aatacgctgc gtttcggcag aactaaactg cccgagacgt tccgacgtcc
    18181 gcgccgccat caccataccc gcagcgaccg cttcaccatg taaccaattg ccatacccca
    18241 tttcagcttc aatggcatga ccaaaggtgt gtcccagatt cagtaaagca cgtaacccgg
    18301 tttcgcgctc gtcggcggcg acaacttctg ccttcagttc acaacaacgg cgaatacagt
    18361 acgccattgc cggaccgtcc agacgcaaca acgcatccag attctcttcc agccagttaa
    18421 aaaacgcacc gtcaagaata atgccgtatt tgatgacttc tgccagcccc gacgctaact
    18481 cacgcggggg aagcgttttc agacagtcga gatccaccac cactgaagca ggctggtaga
    18541 acgcgccaat catgttttta ccgaggggat ggttgaccgc agttttgccg ccaacggagg
    18601 aatcgacctg cgacagtaac gtcgtcggga cttgaatgaa acgaacaccg cgctgataac
    18661 tcgccgccgc gaagccggtc agatcgccca ctacgccgcc gccaagcgcc accagcgtag
    18721 tatcgcgacc atgcggcttt tgtaacaacg ccgtaaagac ggtatcgagt acagccaggc
    18781 ttttatactg ctcgccgtca gggaggataa cgctatcgac gttaacaccc gcctgttcaa
    18841 gtacgccgcg gaccttatcg agatacagag gagccagggt ttcgttggtg accaacatga
    18901 cctgctcgcc cgatttcagc ggtaagaatg aagctggttc attaaacaaa ccagatgcga
    18961 tggtaattgg gtaactacgt tccccgagag taacgacaat cctctccata acgcgacatc
    19021 caccttaatt actgtacccg cagacgagtg tatataaagc cagaattagt tgctttccag
    19081 catgtgaata atctggtttg caaccacttt agcgctttga tcatcagtac gaatggtcac
    19141 gtcggcaatc tcttcataca gcggattgcg ttcattggcc aacgcttcca gaacttcacg
    19201 cggcggtgtt tcaacgtgca gcaacgggcg ttttttatca cgctgcgtgc gtgcaagttg
    19261 cttttcgatg gtcgtttcaa gataaacgac aacgccacga gcggaaagac ggttacgcgt
    19321 ttcacgggat ttcacagagc cgccgccagt agccagcaca ataccctgtt tctcggtcaa
    19381 ctcattgatg accttttctt cgcgatcgcg gaagccttct tcgccttcta aatcgaaaac
    19441 ccagcccaca tcagctccgg ttcgtttctc aatctcttga tcggaatcgt aaaattccat
    19501 attgagttgt tgagctaact ggcgcccaat agtgcttttt ccggcaccca taggcccaac
    19561 cagaaagata ttgcgtttct ctgccatttt ttcggtacta ctaagactat tcgttaatga
    19621 taaacccgct tcgctcagag agcgccgcag gacatgaact gaaacctcat aagatattgc
    19681 gagagtcaga ctgaaaatta tctcaatact ccagcgggtt tggcaactga ataaatcacc
    19741 catccctctg catatctggt cgctgcaagc gcgctgcctt gctaccaccg ctctggcgat
    19801 aaatcaccgg gtaagattag cgtaaaaaag acagcaaaat gccgcctgaa tgataaatca
    19861 tcatcatgga acgcaggcag cgaagagcac caactctcaa atcggtactc cttgtatgct
    19921 aaatacctgc gcgcgtcaaa tagatgaaac acgttcagca taaaaacatt accgctttta
    19981 cggctgttta ctcactggaa accagtcgtg gcgtgataaa caccactaac tcgcgtcgtt
    20041 catcttcttt tccgtcatga cgaaataatt gcccgaacca gggaatgtcg ccaagcaacg
    20101 gtacgctatc ctgacccgat ttatttttac gggtaaaaat gccgcccagc gccaacgttt
    20161 ctccgctttt gacctcgacc tgcgtttcga tctcctgctt atcaatcgcc agcacttcgc
    20221 catcggcctg ctgtagcacc tgccccggaa cgttctggct gatgtgtaat ttcagccgga
    20281 tgcgaccttt ttgtaacacc gtgggcgtga cctccatccc caggacggcc tctttaaatt
    20341 ccaccgacgt cgcgccactt tccccgctgg aaacctgata tggaatttcg ctcccctgtt
    20401 taatgctggc aggctgaaga tgtgaggcca gcagacgcgg actggcgata atatccagct
    20461 gctgtttttg ttcgagcgcg gaaagctcaa gatccagcaa acgtccgttg atgcgcccaa
    20521 tgttaaaacc gacatgcgtt gtcgccgtcg ctacggagag gtcgctacca agcgtggtga
    20581 cttgcccaac gccaccagcg tgttgcgcat cggccagcgt ccatttcacg cctaactcac
    20641 gcaaactttt ttcattaatg gtgacaatat gcgccgacag ctcaacctgc ccgaccggca
    20701 gatccatttg cgctacccac tgttcaagcg cgcttaacgc cgttttgtta tctcgtagca
    20761 aaaggcgatt ggtgcgttta tcgacggtca tactcccttt ggcactcagt agcttctccc
    20821 ccgctttcgc cagttctccc gcgtcggcgt attgcagggt tatactgcga ttttccagcg
    20881 gcagatttgc ctgcgcccgc gcctgctccg cctcctggcg ggcgatattg ttattctgcc
    20941 aggcaatgga atgcactgag agaatgttgt cttcctgccg cgttatcagt ccggcgcttt
    21001 tcactacagt ttgtagtgcc tgcttccagg gaacatctgt tagatgtaac gacaccgtac
    21061 cgctgacgtc tggcgacacg accaggttca acttctcctg ttcagccagc gcctgcaaca
    21121 cctgagctac cggaacgtca tccaccatca gcgtcacttt ttgcggcttt gccgcctgta
    21181 cgccgggtat cagcatcaac agtagtgcgg ctatccattg cttcatttgt atctccttgc
    21241 cgttgccaca accattgtgg cggttcgcag tttgtcccgg tacccagcgt tagtacgtct
    21301 ggcgtcagct gtaaaattgt ccagccgttt tccagcacat cgttttgctg cacccgtcgc
    21361 catttctttt gcccgtcttt tattacaccg atgatgcgct cgcctcgccc taccatcccc
    21421 tgatagcgcc actggctaag ttcgctaatc cggcatagat cttccggcgg tttaaaaggg
    21481 tcacgcatac cggttaaaag gcacaatgca atacctgcca acaaccagcg tttaaccctc
    21541 atgcggcgtc tccagttgta gcgtgaacaa aagatcatca ccttccacgc ttaacgaaaa
    21601 acggctcacg ctgacgttgc gctctgccag ccgtgtaaat gccgacggca ctgcttccca
    21661 cagcgttttc aacgccaact cgcctccctg cgcggatgga tgccaggaaa ccagttgcgc
    21721 gccggataac tgaaaatcca gtggcgaaaa gggcagcgtt ttttcctcgc tgaagggagt
    21781 ggtgtctacc aggcgataca gtgcggccca ctgctggtga tgacttgccc gtagtcgaat
    21841 taatgcgtcg cgctcttcat ggtgtgtcga tgacagaaaa atgagcgtaa ctaacatcag
    21901 caaccagaat gcccagcaaa actggcggag gcggggtgat gtggcgaacc accagtcaaa
    21961 gaacatgttc atcgctaacc tttcttgtta actgatactc aaattgccag cgtccctgcg
    22021 catcctgctg cgtggccccc cgctgattga gatgaaaaga agcatcctgg cgaagtgacg
    22081 tttctagtgc gtttaacgcg gtaatgcttg ttgtaagccc cttgatctcc agcgttccct
    22141 gctgccagct tatcgttgtc agccaggcgt gttcaggtaa aagagccgcc agtgcttcca
    22201 gcgcagattg ccagtcgcgg gtaaattgtc gctggcgctg cctttgcaaa cgctgctcac
    22261 gaatttgttg gcgctccagc aaacgtggtt tcgttatctg taagctgtgg gcgagttgtt
    22321 gttccccctg aagcaaaacg gcgtctacgc gagcttcggc gctgcttgtc agacgcagta
    22381 ttagcgttat cccgacggcc agcagcagag gcgcaacgaa catcagcaac cagaaacgca
    22441 gaaaagcggt ccggcgttgc tgtcgccagg gcaaaaaatt aattggcagg ttcatcagta
    22501 cgccttccca agcgccagcc ccagcgcgat ggcaaagtct ccacctggcg gcggtagcgg
    22561 cggctgacga acagaaacgg cctcccaggg atcaaatccg ccttcgccac atatcgcgac
    22621 gctgtcggga tcaacggata acgctgccgc cagctccttc gcgctagtca tccctaccgc
    22681 cagtttgcgc ccccagcgat agcgtgtcgc ccacagccac tgttcgttat cacgccaggc
    22741 cagacattgc tgatgagaag gtaaaaaagg caggaattgc tgtaatgcac tggcatccgg
    22801 ggtgatcgca ctaacatgaa cacgcaacct ttctgccagc gtaagcagcg ttgccagctc
    22861 tttgctttgc gcggcagtca cgttataagc ggggctgagt gagtcttcgc tataatcgaa
    22921 gcgcagggag tccggatcca tatccagctc gcgggccatc gtccctgaca gccaggccgt
    22981 ttgctcccgc tcaccaaggg acatcgacgg gcgcggaaat gaccgctgta atgtgcgact
    23041 ggcgggaaac gccaacataa tgtgatgacg ctgcggcagt tcgcgactcc aaggtaacaa
    23101 cgttttagcc agctgctgcg catcaacaat ccgcccatct ttgataatgt cgttctccag
    23161 cggcaaccgc caccagcgtt gcaaaaagca ttcttttgcg ccccgtacga tcgcaaccgc
    23221 taccgcttct tgctgttgta aatgcaaacc aatttgccag atcttaaatg ccattgtgat
    23281 gatctcctta tcacccgtca ctctgacggg tatatcaatg cgtctggctt gcctttatac
    23341 taccgcgcgt ttgtttataa actgcccaaa tgaaactaaa tgggaaattt ccagtgaagt
    23401 tcgtaaagta ttttttgatc cttgcagtct gttgcattct gctgggagca ggctcgattt
    23461 atggcctata ccgctacatc gagccacaac tgccggatgt ggcgacatta aaagatgttc
    23521 gcctgcaaat tccgatgcag atttacagcg ccgatggcga gctgattgct caatacggtg
    23581 agaaacgtcg tattccggtt acgttggatc aaatcccacc ggagatggtg aaagccttta
    23641 tcgcgacgga agacagccgc ttctacgagc atcacggcgt tgacccggtg gggatcttcc
    23701 gtgcggcaag tgtggcgctg ttctccggcc acgcgtcaca aggggcgagt accattaccc
    23761 agcagctggc gagaaatttc ttcctcagtc cagaacgcac gctgatgcgt aagattaagg
    23821 aagtcttcct cgcgattcgc attgaacagt tgctgacgaa agacgagatc ctcgagcttt
    23881 atctgaacaa gatttacctc ggttaccgcg cctatggtgt cggtgctgcg gcacaagtct
    23941 atttcggtaa aacggtcgac caactgacgc tgaacgaaat ggctgtgatc gccgggctgc
    24001 cgaaagcgcc ttccaccttt aatccgctct actcaatgga tcgtgccgtc gcgcggcgta
    24061 acgtcgtgct gtcgcggatg ctggatgaag ggtatatcac ccaacaacag ttcgatcaga
    24121 cacgcactga ggcgattaac gctaactatc acgcgccgga gattgctttt tctgcgccgt
    24181 acctgagcga aatggtgcgc caggagatgt ataaccgtta tggcgaaagt gcctatgaag
    24241 acggttatcg catttacacc accatcaccc gcaaagtgca gcaggccgca cagcaggcgg
    24301 tacgtaataa cgtgctggac tacgacatgc gccacggcta tcgcggcccg gcaaatgtgc
    24361 tgtggaaagt gggcgagtcg gcgtgggata acaacaagat taccgatacg ctgaaggcgc
    24421 tgccgaccta tggtccgctg ctgcctgccg cagtcaccag cgccaatcct caggaagcga
    24481 cggcaatgct ggcggacggg tcgactgtcg cattgagtat ggacggcgtt cgctgggcgc
    24541 gtccttaccg ttcggatact cagcagggac cgacgccgcg taaagtgacc gatgttctgc
    24601 aaacgggtca gcaaatctgg gttcgtcagg ttggcgatgc atggtggctg gcacaagtgc
    24661 cggaagtgaa ctcggcgctg gtgtcgatca atccgcaaaa cggtgccgtt atggcgctgg
    24721 tcggtggctt tgatttcaat cagagcaagt ttaaccgcgc cacccaggca ctgcgtcagg
    24781 tgggttccaa catcaaaccg ttcctctaca ccgcggcgat ggataaaggt ctgacgctgg
    24841 caagtatgtt gaacgatgtg ccaatttctc gctgggatgc aggtgccggt tctgactggc
    24901 agccgaagaa ctcaccaccg cagtatgctg gtccaattcg cttacgtcag gggctgggtc
    24961 agtcgaaaaa cgtggtgatg gtacgcgcaa tgcgggcgat gggcgtcgac tacgctgcag
    25021 aatatctgca acgcttcggc ttcccggcgc aaaacattgt ccacaccgaa tcgctggcgc
    25081 tgggttcagc gtccttcacc ccaatgcagg tggcgcgcgg ctacgcggtc atggcgaacg
    25141 gtggcttcct ggtggacccg tggtttatca gcaaaattga aaacgatcag ggcggcgtga
    25201 ttttcgaagc gaaaccgaaa gtagcctgcc cggaatgcga tattccggtg atttacggtg
    25261 atacgcagaa atcgaacgtg ctggaaaata acgatgttga agatgtcgct atctcccgcg
    25321 agcagcagaa tgtttctgta ccaatgccgc agctggagca ggcaaatcag gcgttagtgg
    25381 cgaagactgg cgcgcaggag tacgcaccgc acgtcatcaa cactccgctg gcattcctga
    25441 ttaagagtgc tttgaacacc aatatctttg gtgaaccagg ctggcagggt accggctggc
    25501 gtgcaggtcg tgatttgcag cgtcgcgata tcggcgggaa aaccggtacc actaacagtt
    25561 cgaaagatgc gtggttctcg ggttacggtc cgggcgttgt gacctcggtc tggattggct
    25621 ttgatgatca ccgtcgtaat ctcggtcata caacggcttc cggagcgatt aaagatcaga
    25681 tctcaggtta cgaaggcggt gccaagagtg cccagcctgc atgggacgct tatatgaaag
    25741 ccgttcttga aggtgtgccg gagcagccgc tgacgccgcc tccgggtatt gtgacggtga
    25801 atatcgatcg cagcaccggg cagttagcta atggtggcaa cagccgcgaa gagtatttca
    25861 tcgaaggtac gcagccgaca caacaggcgg tgcacgaggt gggaacgacc attatcgata
    25921 atggtgaggc acaggaattg ttctgattaa aaaggcgctt cggcgccttt tcagttggct
    25981 gacaaagtgc atttgtttat gccggatgcg ggtgaacgcg ttatccggct aacaaaatcg
    26041 tgaaaaatca atatattgca ggaaacccat aagcctgata agcgttgtgc atcaggcaaa
    26101 cttcacgcat ttacactcgc ccctgccctt tcaaccattc gcgcacgagg aacagcgcgc
    26161 tgacattacg cgcttcgttg aagtcagggt cttccagcaa atccatcata tgcgccagcg
    26221 gccagcgcac ctgtggtagc ggctctggct catcgccttc cagtgattcc gggtagagat
    26281 cttgcgctac cacgatattc attttgctgg aaaaataaga cggtgccatg ctgagcttct
    26341 tcaaaaaagt cagatcgttc gcaccaaatc caacctcttc ttttagctcg cggttagcgg
    26401 cttcgtagac gctttcaccc ggatcaatta aacctttcga aaaacctaat tcgtaggatt
    26461 cagttcccac tgcgtattcg cggatcagga tcaggtgatc gtccacaatc ggcacaatca
    26521 tcactgcttc ccggttggtt ggacgcattc gttcataaac acgccgcacg ccattgctga
    26581 actccagatc cacgctctcg acggtaaaca gtcgggaacg ggctacagtt tcaacattca
    26641 gaatggtggg tttttgtaat gatttgctca tcgtgggatc tatgctgtga aatcagcggt
    26701 tattgtgcga tatcggacac gctttcggca atgtgaattg catgttattt acatttatgt
    26761 aacttaataa ataatcgtcc tcaaatcaaa ttaaaagtca ataggttgaa ataactccag
    26821 gaatttgctg atattccgcc ttcagagggt ttgctatgat cagcggttac tgtgatgtgc
    26881 ttaatgatgc tcaagttaaa ctccacgctt gccgatagcc aaccgcagaa tcatgtattg
    26941 tgtctggtgc gactgaccac gcctgacaga ctaagtaaga tggggaaagc atgagcacca
    27001 ttgtgatttt tttagctgct ttgctggcct gctcactact tgcgggatgg ctgataaaag
    27061 tgcgatccag acggcgtcag ctgccctgga ccaacgcctt cgcggatgcg caaacgcgta
    27121 aactcacacc tgaagaacgt agcgccgttg aaaattatct tgagagcctg acgcaggtat
    27181 tacaggtgcc tggcccaacg ggagctagcg cggcaccgat ctctctggcg ctgaatgccg
    27241 aaagcaacaa cgtcatgatg ctgacacacg ctatcacgcg ttacggcatc tctaccgacg
    27301 atccgaataa atggcgttac tacctcgatt ccgtagaagt ccacctgccc cctttctggg
    27361 aacaatacat caacgatgag aataccgttg aactgattta taccgattcg ctgccgctgg
    27421 ttatttcact caacggtcat acgctgcagg agtacatgca ggaaacgcgc ggctatgcct
    27481 tgcaacctgt tccgtccacg caggcgtcga ttcgcgggga agagagtgag caaatcgagc
    27541 tactcaatat tcgcaaagaa acgcatgaag aatatgcgct gagtcgtccg cgcgggctgc
    27601 gtgaagcgtt gctgatcgtc gcctccttcc tgatgttctt tttctgcctg attaccccgg
    27661 atgtatttgt tccgtggctg gcaggcggcg cattactgct gctgggcgca ggtctgtggg
    27721 ggctattcgc gcccccggca aaatcctccc tgcgagaaat tcattgtctg cgcggtacac
    27781 cccgtcgttg gggattgttt ggcgaaaacg atcaggaaca gatcaacaat atttcactcg
    27841 gtattatcga cctggtctat cccgcacact ggcagccata cattgctcag gatctcggtc
    27901 aacaaaccaa tattgatatc tatctcgacc gccacgtagt gcgtcagggg cgatatcttt
    27961 cgctgcatga tgaagtaaaa aactttccgt tacagcactg gctgcgcagt acgattatcg
    28021 ctgcgggctc gctgctggtg ctgtttatgc tgttattctg gatcccgctg gatatgccgc
    28081 tgaaattcac tctctcatgg atgaaaggcg cgcagaccat tgaagccacc agcgtaaaac
    28141 aactggctga cgctggcgtg cgggtaggcg atacattgcg tattagcggt acgggaatgt
    28201 gtaatattcg cacttccgga acctggagcg cgaaaaccaa ttcacctttt ttaccgtttg
    28261 actgctcgca gatcatctgg aatgacgccc gctcattacc attaccagaa tctgaactgg
    28321 tcaacaaagc gacggcattg actgaagcag ttaatcgcca gctgcaccct aaaccggaag
    28381 atgaatctcg cgtcagtgcc tcattacgtt cagcgattca aaaatccggc atggtattgc
    28441 ttgatgattt tggcgacatt gtactgaaga cagcggattt atgttctgcc aaagatgact
    28501 gtgtgcgact gaaaaatgcg ctggtcaatc tcggcaacag taaagactgg gacgcgctgg
    28561 taaaacgcgc caacgccggg aagctcgatg gcgtgaatgt gttattacgc ccggtgagtg
    28621 cggaatcgct ggataacctg gtggcaacct ccaccgcgcc gtttatcacg catgaaacgg
    28681 cgcgagcggc acaatcacta aacagtccgg cccccggcgg attcctgatt gtcagcgacg
    28741 aaggcagcga ttttgttgat cagccctggc cttcggcatc actttacgac tacccgccgc
    28801 aagaacagtg gaacgctttc cagaaactgg cacaaatgct gatgcatacg ccgtttaacg
    28861 ccgaaggtat cgtcacaaaa atcttcactg acgccaatgg tacgcagcat attggccttc
    28921 atccgatccc ggatcgttcc ggcctgtggc gctatctcag caccacattg ctgctactga
    28981 cgatgctggg tagcgccatt tacaatggcg tacaggcctg gcgtcgttac cagcgtcatc
    29041 gcactcgcat gatggagatt caggcctatt atgaaagctg cctgaacccg caactgatca
    29101 ccccttcaga aagccttatc gaataacacg tttgcgcggc aggttatgct accctgtcgc
    29161 gcaaattgct tcactctgga gatttccctc atgcatatca acattgcctg gcaggacgta
    29221 gataccgttc tgctggatat ggacggcaca ttgctcgacc tcgccttcga taactatttc
    29281 tggcaaaagc tggtgcctga aacatggggc gcgaaaaacg gggttacgcc acaggaagcg
    29341 atggaatata tgcgccagca atatcacgac gtacagcata cgctaaactg gtactgtctt
    29401 gattactgga gtgagcaact gggtctggat atctgtgcga tgaccaccga gatgggaccg
    29461 cgtgccgtac tgcgtgaaga taccattccg tttcttgagg cactgaaagc cagcggtaag
    29521 cagcgaattt tgctcaccaa tgcgcatccg cacaacctgg cggtaaaact tgagcatacc
    29581 ggtctggacg cacaccttga tttattactt tccacccaca catttggtta tccgaaagag
    29641 gatcagcggt tatggcatgc ggtggccgaa gctacgggtc tgaaagctga aagaacgctg
    29701 tttattgatg acagcgaagc gattctcgat gctgccgcgc aatttggtat tcgttactgc
    29761 ctcggcgtga ctaatcctga ttccgggatt gccgagaaac agtatcaacg ccatccgtca
    29821 ctgaatgact accgccgcct gatcccctcg ctaatgtgaa ggagacgcca tgaaagaaaa
    29881 acctgctgtt gaggttcgac tggataaatg gctatgggct gcccgttttt ataaaacccg
    29941 cgcgctggcc cgtgaaatga ttgaaggcgg taaggtgcat tacaacgggc agcgcagcaa
    30001 gccgagcaaa atcgtcgagc tgaatgccac gctcactctg cgccagggaa atgacgaacg
    30061 cacggtgatt gtaaaggcga ttactgaaca gcgtcgcccc gccagcgagg cagccttgct
    30121 gtatgaagag actgcggaaa gtgtagagaa acgcgaaaaa atggcgatgg cacgtaaact
    30181 taatgcctta accatgccgc acccggaccg acgcccggac aaaaaagagc gccgcgacct
    30241 gttacgattt aaacacggcg acagtgaata actgtcacct gcaagagaga tgattatgcc
    30301 gcaacatgac caattacatc gctatctgtt tgaaaatttt gccgttcgcg gcgaactggt
    30361 aaccgtttcg gaaaccctgc aacagatcct tgagaaccac gattatccgc agcccgttaa
    30421 aaacgtgctg gcagaactgc tggttgcgac cagcctgtta accgctacgc tgaagtttga
    30481 tggtgatatc accgtacagc tgcaaggcga cggtccgatg aatctggcgg ttattaacgg
    30541 taacaataac cagcaaatgc gcggtgtggc gcgcgtgcag ggcgaaattc cagaaaatgc
    30601 cgacctgaaa acgctggtcg gcaatggtta cgtggtgatc accattaccc cgagcgaagg
    30661 cgaacgctat cagggcgtgg ttggtctgga aggtgatacc ctggcggcct gcctggaaga
    30721 ttactttatg cgttctgaac agctgccgac gcgcctgttt attcgcaccg gcgacgtaga
    30781 cggcaaaccg gctgcaggcg gtatgttgtt gcaggtaatg cctgcgcaaa atgcccagca
    30841 ggacgacttt gaccacctgg cgacgctaac cgaaaccatc aaaaccgaag aactgctgac
    30901 cttaccggca aacgaagtgt tgtggcgttt gtatcacgaa gaagaggtga cggtttacga
    30961 tccgcaggat gtggagttca aatgcacctg ctcgcgtgaa cgttgcgccg atgcgctgaa
    31021 aacgctgcct gatgaagaag ttgatagcat cctggcggaa gatggcgaaa ttgacatgca
    31081 ttgtgattac tgcggtaacc actatctgtt caatgcgatg gatattgctg aaatccgcaa
    31141 caacgcgtct ccggcagatc cgcaagttca ttaatgagta tgtccggcag agagggctct
    31201 gccggatttt acgacgtaat ggcctgatgt gctgcgctta ccaggcctgt gaaataaatc
    31261 gcaaattatt gttggtcaga tacagcgtcg tatccgttaa aagagttgtc acttacttaa
    31321 gtttatttcc agcgtttcac catctaccgt cagatcgcgc cacgctttcg gcctcgtccc
    31381 cagttttcca tccttcgcag ctgtgagtaa tttttctttc gttatttcgt tcggaagtaa
    31441 actcatatca agcgcgtccc attcttttct gtctgagtca aagccataga caatgactct
    31501 gtcatcatta aaagcaaaca ggatccgctc cgcctgacca tcactattca gatcttgctc
    31561 gaccaggacg caggcgtctt tttcaataca ggtcatcacg ttatagcgat cctggattaa
    31621 ggccgaccag aatgtcgcat ccggtttaac agaaccaggg gcaattaaca cattttcggc
    31681 taatactttt tccgatacct gttcctgaag atgttgctct ccatctaacg ccatcagcag
    31741 atcccgcgcg cgtttcgggt ctttcataaa cccggcatcg cttttcagcg actcaagcgc
    31801 cgcacgtcca tagcgaccgc tctgttcgag catgtagata gttacctggt ctggcgtgtt
    31861 tttgccgctc tgataacgcg ccatatgact gttcacgcta atacgcatac tgtccagcac
    31921 cggcgaatta agaagcacca gtatcaccaa caccaataac gaaaccgcaa ggttcacttt
    31981 gccctgaaga acaaggggat tttgcccatt acgccagacg atactgacaa aataccccag
    32041 cgaccacacc agtaacacca gcaccgccag cgcgccctgc aagcggtcga cagtccagcc
    32101 gtactgagcg acccgcagcc ataacgccca ggcggcgacg aacacataca gcggagcaac
    32161 cagcaaagcg gttttaatca ggcaacgcaa cggccccgtc cagggaagtg acgctttttg
    32221 cggatcgcgg acaatagcca ttaagatcaa ttgcaaaaag gccagcgtta acagcaaccc
    32281 ggcggcggag atgtggcgag aaatcgcgct caggcccgta aacggcagag tgatgataaa
    32341 catcagggtt agcaatgata ctaacggcag caaccccgtg gcgattaacg tgaacaactt
    32401 ttgaatagag tcgattaaac gtgactgtgt gcgcgcgaga atcaccgcca gtgcggtaac
    32461 cagacctaat gtaagataca taaaccagtc ggttgcaaaa aagagcgtat tgaaaaactt
    32521 gatgccaaca agtttaaaca actcacccca cagtaaaagc accagccacg tcaggccatt
    32581 ggcgagaaaa atcaccagta atataagtac gttatgccat actgactggt aaaagtagct
    32641 atagcggcta ctgccattgc ggatacgaag gctttgttgt atccacggta gcaacaacat
    32701 tgccatcaac agcagatagc agccaaaatc ccaaatcgcc ttttcagctc tccaggggtt
    32761 catgccatca gtttgccact tcagccaacc gctcatccct gtcgtggcaa taaacaccag
    32821 cgccagccat ccccaaaggc gcttctgttt aaaagagatc acggagaaga gtaaaacgga
    32881 tgaaaacgca acggtcgcag gcacgccata aacaatccaa ctgtgatttt ttccggcaag
    32941 ccaggcgatc aacaggtagc aaaccagccc ctgaagtaat ccggtagcaa tcatccccca
    33001 gcgtgttgcc ggtgaaagtt caacgttatc catgttggtt atccagaatc aaaaggtggg
    33061 ttaattatcg catccgggca gtagtatttt gcttttttca gaaaataatc aaaaaaagtt
    33121 agcgtggtga atcgatactt taccgattga atttgcatca atttcattaa ggaatgcgat
    33181 tccactcaca atattcccgc catataaacc aagatttaac cttttgagaa cattttccac
    33241 acctaaaatg ctatttctgc gataatagca accgtttcgt gacaggaatc acggagtttt
    33301 ttgtcaaata tgaatttctc cagatacgta aatctatgag ccttgtcgcg gttaacaccc
    33361 ccaaaaagac tttactattc aggcaataca tattggctaa ggagcagtga aatgcgcgtt
    33421 aacaatggtt tgaccccgca agaactcgag gcttatggta tcagtgacgt acatgatatc
    33481 gtttacaacc caagctacga cctgctgtat caggaagagc tcgatccgag cctgacaggt
    33541 tatgagcgcg gggtgttaac taatctgggt gccgttgccg tcgataccgg gatcttcacc
    33601 ggtcgttcac caaaagataa gtatatcgtc cgtgacgata ccactcgcga tactttctgg
    33661 tgggcagaca aaggcaaagg taagaacgac aacaaacctc tctctccgga aacctggcag
    33721 catctgaaag gcctggtgac caggcagctt tccggcaaac gtctgttcgt tgtcgacgct
    33781 ttctgtggtg cgaacccgga tactcgtctt tccgtccgtt tcatcaccga agtggcctgg
    33841 caggcgcatt ttgtcaaaaa catgtttatt cgcccgagcg atgaagagct ggcaggtttc
    33901 aaaccagact ttatcgttat gaacggcgcg aagtgcacta acccgcagtg gaaagaacag
    33961 ggtctgaact ccgaaaactt cgtggcgttt aacctgaccg agcgcatgca gctgattggc
    34021 ggcacctggt acggcggcga aatgaagaaa gggatgttct cgatgatgaa ctacctgctg
    34081 ccgctgaaag gtatcgcttc tatgcactgc tccgccaacg ttggtgagaa aggcgatgtt
    34141 gcggtgttct tcggcctttc cggcaccggt aaaaccaccc tttccaccga cccgaaacgt
    34201 cgcctgattg gtgatgacga gcacggctgg gacgatgacg gcgtgtttaa cttcgaaggc
    34261 ggctgctacg caaaaactat caagctgtcg aaagaagcgg aacctgaaat ctacaacgct
    34321 atccgtcgcg atgcgctgct ggaaaacgtc accgtgcgtg aagatggcac tatcgacttt
    34381 gatgatggtt caaaaaccga gaacacccgc gtttcttatc cgatctatca catcgataac
    34441 attgttaagc cggtttccaa agcgggccac gcgactaagg ttatcttcct gactgctgat
    34501 gctttcggcg tgttgccgcc ggtttctcgc ctgactgccg atcaaaccca gtatcacttc
    34561 ctctctggct tcaccgccaa actggccggt actgagcgtg gcatcaccga accgacgcca
    34621 accttctccg cttgcttcgg cgcggcgttc ctgtcgctgc acccgactca gtacgcagaa
    34681 gtgctggtga aacgtatgca ggcggcgggc gcgcaggctt atctggttaa cactggctgg
    34741 aacggcactg gcaaacgtat ctcgattaaa gatacccgcg ccattatcga cgccatcctc
    34801 aacggttcgc tggataatgc agaaaccttc actctgccga tgtttaacct ggcgatccca
    34861 accgaactgc cgggcgtaga cacgaagatt ctcgatccgc gtaacaccta cgcttctccg
    34921 gaacagtggc aggaaaaagc cgaaaccctg gcgaaactgt ttatcgacaa cttcgataaa
    34981 tacaccgaca cccctgcggg tgccgcgctg gtagcggctg gtccgaaact gtaatgattt
    35041 gaagctggag aatatctatc cagtatctta tagaaagcaa aacgggaggc accttcgcct
    35101 cccgtttatt tacccttctt ttgtcgtgcc ctgcgcccgc gttaccggca ctggcagcca
    35161 ggcgcgaatg gaaagcccgc cccgctcgct ggtgccaagc tccagcatcc cgttatggtt
    35221 atccacgata cgctgcacaa ttgccagccc taatcccgtg ccgctaatgg tgcgcgcact
    35281 gtcgccgcgg acaaacggct ggaacaggtg cttacgttgt tccggcgcaa ttcccggacc
    35341 gtcatcttcc acctggaacc aggcgcgatt cggctccgtt ccgctgctga ctttgatcca
    35401 gccattgcca taacgggcgg cgttgaccac catattcgcc accgcgcgtt tgatcgacag
    35461 cgggtgcatt ttcacttcaa tgctgccggg gtaaagcgcg gtttcaattt cccgctcata
    35521 gccactttcg gcagcaatca cctcaccgag tactgcatta agatccgcca tttccatcgg
    35581 catctcctgc ccggtgcgca ggtagtcgat aaactgctca atgatggcgt tgcactcttc
    35641 gatatcttta ttgatcgatt ctgccagata gccatcctgc tcgctcatca tctcagtcgc
    35701 caggcgaata cgcgtcagcg gcgtgcgcaa gtcgtgactt acccccgcca tcagcagcgt
    35761 gcggtcatcc gccagttgct taacaccagc cgccatatgg ttaaaggcac gggtaacgga
    35821 acgcacctcc gaagcgccat attcacgcag cggcggcgga ataatccctt taccaacctg
    35881 caaggctgcg tgttcgagat cgaccaacgg tcggttctgg atacgaataa acagccacgc
    35941 cccgcctatc gccaatagca taatcgccag cgtatagcgg aacagcggag agaaatcgcc
    36001 ctgatgaatt tcggtcagcg gcacgcgtac ccagatattg ggcgacagcc aggttttcag
    36061 ccagacgaca ggcgaacttt tgttgacctc aacgcgcact tccgtcgggc cgcccagttg
    36121 ctgtgccatc tgatggctta agaattcata gtgttgcgcc caacgcagac ctgcctcttc
    36181 ggcagcctcg ttggagtaga gagagatccc cagctcacgg tagatctccc gacggaaagc
    36241 gggaggcaca accaactgcg tgccgtcctc cagttgcagt ttgtcggtca tcaacatacg
    36301 cacttcgtac gcgaggactt tattaaactg ctggaggctc ggcaaaatcg cgaagttcag
    36361 caccaccaga taagtcgtca ccaggctggc gaacagcaag gtgacgatga gcaataacgt
    36421 acgggcaaat gaacttcgtg gcgagaagcg caatcgcctc atgctttaga gccgtccggt
    36481 acaaagacgt agcccaggcc ccaaacggtc tgaatgtaac gcggatgcgc tggatcttct
    36541 tctaccatgc ggcgcagacg cgaaatctgc acgtcgatgg agcgttccat tgcggaatat
    36601 tcacgaccac gggcaaggtt catcagctta tcgcgggaga gcggctcacg cggatggctg
    36661 accagtgcct tcagtaccgc aaactcaccg ctggtgagcg gcatcggctc atcttcgcgg
    36721 aacatttcgc gcgtaccgag gttaagtttg aacttaccga aagcaattac cgcctcttcc
    36781 tgtgacggtg cgcctggcag ttcgttcgcc tgacgacgca gcaccgcacg aatacgggcc
    36841 agcagttcac gcgggttaaa cggttttgga atgtagtcgt cagcgccaat ctccaggcct
    36901 acgatacggt ccacttcttc ccctttcgcc gtcaccataa tgatcggcat cgggttgctc
    36961 tgactacgaa gacgtcggca aatcgacaag ccatcttcac caggtaacat taaatccagt
    37021 accataagat ggaaagattc acgagtcagc aggcgatcca tctgttctgc attagcgacg
    37081 cttcgaacct ggaagccttg ttcggtgaga taacgttcca gcagcgcacg caggcgcatg
    37141 tcgtcatcga ccaccagaat cttgtagttc tcttgcattg tttgtactcc caaaggttcg
    37201 caacaatttg taagcgtgta ttcttaaaaa agctcacgtt cgtcaccagc taaatctggt
    37261 atgaatttca gcctaaattg ttacaaagca tattaaacag cagcttaagt atacaattta
    37321 ttcggcgaaa cattattgat tctgttgata tgatcacgtt atacccaatg tgcgcattat
    37381 caaacagaca aagggaatca acgagatgaa aacgcccctg gttacccggg aagggtatga
    37441 aaaactcaaa caagagctta attatctctg gcgtgaagaa cgcccggagg tcacaaaaaa
    37501 ggtgacctgg gccgcaagtc tgggcgaccg cagcgaaaat gctgactatc agtataataa
    37561 aaagcgtctg cgtgaaatcg accgtcgcgt gcgctatctc actaaatgca tggaaaatct
    37621 caaaatcgtc gattactccc ctcagcagga aggcaaagtc ttttttggcg cgtgggtgga
    37681 gattgaaaac gacgatggcg tgactcaccg tttccgtatt gtcggctacg atgaaatttt
    37741 tggccgtaaa gattacatct ctatcgattc cccgatggcc cgcgcattgc tgaaaaaaga
    37801 agtcggcgat ctggcggtgg tgaatacccc tgccggggaa gcgagctggt atgttaatgc
    37861 tatcgagtac gtgaaaccgt aaggaagagt cttaacctcc tgccgatggc tggcattttt
    37921 gccagccagt ccgtataact atcccctgat ttttgatccg aaaagatgaa ctcaaaccat
    37981 gatgaatgat tcgttctgcc gcattattgc gggtgaaatt caggcgcgcc cggaacaggt
    38041 tgacgctgcc gttcgcctgc ttgacgaagg gaataccgtg ccgtttatcg cacgttatcg
    38101 taaggaaatc accggcggtc tggatgacac gcagctgcgt aatctggaaa cgcgtctgag
    38161 ctatctgcgc gagctggaag agagacgtca ggcgatcctc aagtccattt ccgagcaagg
    38221 caaactcacc gatgatctgg cgaaggccat caacgccacc ctaagcaaaa ccgaactcga
    38281 agacctctac ctgccctaca aacctaaacg ccgcacccgc gggcaaatcg ccattgaagc
    38341 agggcttgag ccgttggctg acctgctgtg gagcgatccg tcacacacgc cagaagtcgc
    38401 cgctgcacaa tatgttgatg ccgataaagg cgtggcagat accaaagccg cgctggatgg
    38461 cgcgcgctat atcctgatgg aacggtttgc cgaagatgcc gcgctgctgg cgaaagtgcg
    38521 tgattatctg tggaagaacg cgcatttggt ttctacggtg gtgagcggta aagaagagga
    38581 aggggcgaaa ttccgcgact atttcgatca tcacgaaccg ttgtccacgg tgccttctca
    38641 ccgcgcgctg gcgatgttcc gtgggcgtaa cgaaggcgta ctccagcttt cgctgaatgc
    38701 cgatccgcag ttcgatgagc cgcccaaaga gagctattgc gagcaaatca tcatggatca
    38761 ccttggcctg cgcctgaaca atgccccggc ggatagctgg cgcaaaggcg tagtgagctg
    38821 gacctggcgc atcaaggtgc tgatgcatct ggaaaccgaa ctgatgggta ccgtgcgcga
    38881 acgcgcggaa gatgaagcaa tcaacgtctt tgcccgtaac ctgcacgatc tgctgatggc
    38941 ggcccctgcc ggactgcgtg caacgatggg cctcgatccg ggtctgcgta ctggggtaaa
    39001 agtggcggtg gtcgatgcca ctggcaaact ggtggcgacc gataccattt acccgcacac
    39061 cggacaagcc gcaaaagcag cgatgaccgt tgctgccttg tgtgaaaaac ataacgttga
    39121 actggtggcg atcggcaacg gtacagcttc ccgcgaaact gaacgtttct atctcgacgt
    39181 gcagaagcag ttcccgaaag tgaccgcaca gaaagtgatc gtcagcgaag ctggcgcgtc
    39241 ggtttactca gcttccgagc tggcagcgca ggagttcccg gatctcgacg tttcgctgcg
    39301 tggtgcggta tctatcgccc gccgtttgca ggatccgctg gcggagctgg tgaaaatcga
    39361 tccgaaatct atcggcgtag gtcagtatca gcatgacgtc agccagacgc aactggcccg
    39421 caaactggac gcagtagtag aagactgcgt aaacgccgtt ggcgtcgatc tcaacactgc
    39481 ttctgttccg ctgttaaccc gcgtggcggg cctgacgcgc atgatggcgc aaaacatcgt
    39541 ggcctggcgc gatgagaacg gtcagttcca gaaccgtcag caactgctga aagtgagccg
    39601 tctggggccg aaagccttcg agcagtgcgc gggcttcttg cgcattaacc atggtgataa
    39661 cccgctggac gcgtctaccg tccacccgga agcctatccg gtggtggaac gcattctggc
    39721 agcaacacag caggcactga aagatctgat gggtaacagc agcgaactgc gtaacctgaa
    39781 agcgtctgac tttactgatg aaaaattcgg tgtgccgaca gtaactgaca tcatcaaaga
    39841 gctggaaaaa ccgggtcgcg atccgcgtcc ggaatttaaa accgctcagt ttgccgatgg
    39901 cgtcgagaca atgaacgacc tgcaaccggg tatgatcctc gaaggtgcgg tgaccaacgt
    39961 caccaacttt ggcgcgtttg tcgatatcgg cgtgcatcag gacggcctgg ttcacatctc
    40021 ttcattgtcg aacaagtttg tagaagatcc gcataccgtg gtgaaagcgg gcgacattgt
    40081 gaaggtgaaa gtgctggaag tggatcttca gcgtaaacgt atcgccctga ccatgcgtct
    40141 ggacgagcag cctggcgaaa ccaacgcccg tcgcggcggc ggtaatgaac gcccgcaaaa
    40201 caaccgcccg gcagccaaac cgcgcggacg tgaagcgcag cctgccggta acagcgcgat
    40261 gatggacgcg ctggcggcag caatgggcaa aaaacgttaa acgccctgat tgacccctaa
    40321 acctgcgcga taaggtgtcc acgccgcatc tggcaacgaa cgccttgtct gacataaaag
    40381 tgccggagaa tatctccggc atttttattc cacagccaaa ctcacaatat attccggcaa
    40441 catttatcac ttcattaaca actgaaacct taattaaaca ttagccagtc ctggtaattc
    40501 gctattcgaa ttatattttc gctgcgatat aaccttgagc cacatcaaca ttgagtcaga
    40561 ttattattca aaccaacatt cgcacacatt ttaagtattg ctgatagaaa ccattctcat
    40621 tatcattgtg ctgttgatta tttaatctct ccttcgttgg caaatcatct ggtctcatgt
    40681 cgctgtcaaa cgccccatga ggtagttatc cagttaatga gaaacaagta ggcacctatg
    40741 caatacactc cagatactgc gtggaaaatc actggctttt cccatgaaat cagcccggca
    40801 tatcgccaaa aactgctttc tcttggcatg ttacctggct cctcttttaa tgtggtgcgc
    40861 gtcgctccac tcggcgaccc cattcatatc gaaacccgtc gtgtgagcct ggtattacgc
    40921 aaaaaagatc tggccttatt agaagtggaa gcggtttcct gttaatacgg tgataacaac
    40981 aatgaaaaaa ttaaccatag gcttaattgg taatccaaat tctggcaaga caaccttatt
    41041 taaccagctc actggcgcac gtcagcgtgt aggtaactgg gctggcgtta ccgtcgaacg
    41101 taaagaaggg caattctcca ccaccgatca tcaggtcacg ctggtggacc tgcccggcac
    41161 ctattctctg accaccatct catcgcagac ctcgctcgat gagcaaatcg cctgtcacta
    41221 cattttgagt ggcgacgccg acctgctgat taacgtggtg gatgcgtcta accttgagcg
    41281 taacctgtac ctgacgctac aactgctgga actcggcatt ccctgcatcg tggcactgaa
    41341 catgctcgac attgccgaga agcaaaatat tcgtattgaa attgatgctc tgtcggcgcg
    41401 tcttggctgc ccagtgatcc cgctggtttc aacccgtggt cgcggtattg aagcgctcaa
    41461 gctggcgatt gatcgctata aagctaacga gaatgtggaa ctggtgcatt acgcacagcc
    41521 gctactcaac gaagcagatt cactggcaaa agtgatgcct tccgacatcc cgctgaaaca
    41581 acgtcgctgg ctgggcctgc aaatgctgga aggcgatatc tacagccgcg cctacgccgg
    41641 tgaagcgtcg cagcatctgg atgccgccct cgcccgtctg cgtaatgaga tggacgatcc
    41701 ggcgctgcac attgccgatg cgcgttacca gtgcattgct gccatctgtg atgtggtaag
    41761 caacaccctg acggcagaac ccagccgttt caccaccgcg gtagataaaa tcgtgctcaa
    41821 ccgtttcctc ggtctgccga ttttcctctt tgtgatgtac ctgatgttcc tgctggctat
    41881 caacatcggc ggggcgttac agccgctgtt tgacgtcggc tccgtggcgc tatttgtgca
    41941 tggtattcaa tggattggct acacgctcca cttcccggac tggctgacta tcttcctcgc
    42001 ccagggcctg ggtggcggca ttaacaccgt gctgccactg gtgccgcaga ttggcatgat
    42061 gtacctgttc ctctccttcc ttgaggactc cgggtatatg gcgcgtgcgg cgtttgtgat
    42121 ggaccgtctg atgcaggcgc tgggcttgcc ggggaaatcc tttgtgccgc tgatcgtcgg
    42181 tttcggttgt aacgtaccgt cggtaatggg tgcacgtacg cttgatgcac cgcgtgaacg
    42241 tctgatgacc atcatgatgg caccgtttat gtcctgcggc gcgcgtctgg ctatcttcgc
    42301 agtattcgcg gctgccttct tcgggcagaa cggtgcgctg gcggtcttct cgctgtatat
    42361 gctgggtatt gtgatggcgg tgctgactgg cctgatgctc aagtacacca tcatgcgcgg
    42421 tgaagcgacg ccgtttgtca tggagttgcc ggtctatcat gtaccacacg ttaaaagcct
    42481 gattatccag acctggcagc gtctgaaagg cttcgttctg cgtgctggta aagtgatcat
    42541 catcgtcagc attttcctga gcgctttcaa cagcttctcg ctgagcggga aaatcgtcga
    42601 taacatcaac gactcggcgc tggcgtccgt cagccgggtg atcaccccgg tcttcaagcc
    42661 aattggcgtg catgaagata actggcaggc aacggttggc ctgtttacag gtgccatggc
    42721 gaaagaagtg gtagtgggta cgctcaacac cctctacacc gcagaaaata ttcaggacga
    42781 agagttcaat ccggcagaat ttaacctcgg tgaagagctg ttcagtgcga tagatgaaac
    42841 ctggcagagc ctgaaagaca ccttcagcct tagcgtactg atgaacccca ttgaagccag
    42901 caaaggcgac ggcgaaatgg gtaccggggc gatgggcgtg atggatcaga aattcggtag
    42961 cgcagcagca gcttacagct acctgatttt cgtcctgctg tatgtaccat gtatctcggt
    43021 gatgggagcc atcgcccgtg aatcaagccg tggctggatg ggcttctcca tcctgtgggg
    43081 gctgaatatc gcttactcgc tggcaacatt gttctatcag gtcgccagct acagtcagca
    43141 cccaacttac agcctggtgt gcattctggc ggttatcctg tttaacatcg tggttatcgg
    43201 tctgctgcgc cgcgcgcgta gccgggtgga tatcgaactg ctggcaaccc gcaagtcggt
    43261 aagcagttgc tgcgcagcca gcaccaccgg tgattgccat taatggcttc acttattcag
    43321 gtgcgcgatt tgctggcgtt acggggccgt atggaagcgg cccagataag ccagacattg
    43381 aacactccac agccaatgat taacgccatg ctgcaacaac tggaaagtat gggcaaagcc
    43441 gtgcggattc aggaagaacc tgacggctgc ctctctggca gttgtaaaag ctgcccggaa
    43501 ggaaaagcct gtctgcgcga gtggtgggcg ctgcgttaac cttactccat cgcctgtttt
    43561 ggaaagcccg gtatgcgtct gcatccgggc ttttttgcgt gcggctttcc ataaaaatgc
    43621 aactcttgca gcacggcgta agttcctttg aaagcatctc gcagggatga aaactcgcta
    43681 atgcacagat gtggagtggc gcgtagagtc gcggcattca aacaacaggt gaaggaacgc
    43741 catgagtaaa aagcagagtt ccaccccaca tgatgcgctg ttcaaactct ttttacgcca
    43801 accggacacg gctcgcgatt ttcttgcgtt ccatttaccg gcccccattc acgcgctctg
    43861 tgatatgaaa accctcaagc tggagtcgag cagctttatt gatgacgatc tgcgtgaaag
    43921 ctattccgat gtgctgtggt cggtgaaaac ggaacaagga ccaggataca tctattgtct
    43981 gattgaacat caaagcacct caaacaaact gatcgcattt cgcatgatgc gttacgctat
    44041 tgccgcaatg caaaatcacc ttgatgctgg atacaaaacg ttgccgatgg tggtgctatt
    44101 gttgttttac cacggtattg aaagccccta tccctattcg ctgtgttggc tggattgttt
    44161 cgccgatccc aaactggcaa ggcagcttta tgcctccgca tttccgctga ttgatgtcac
    44221 cgtcatgcct gatgatgaaa tcatgcagca ccgacgtatg gcgctgctgg agttaattca
    44281 aaaacatatt cgtcaacgcg acctgatggg gctggtagag caaatggcct gcttattaag
    44341 tagtggatac gctaatgaca gacaaatcaa agggctgttt aattacatac tgcaaaccgg
    44401 cgacgcggta cgttttaacg attttatcga cggcgttgcc gaacgttcac cgaaacacaa
    44461 ggagagttta atgacaattg cggaaagatt gcggcaggag ggggaacaat ccaaagccct
    44521 gcatatagcc aaaataatgc ttgaatccgg agtccctctt acagacatca tgcgctttac
    44581 cggggtgtca gaagaagagt tggctgcggc gagccagtaa atttctgtct cgccatttca
    44641 aaagttgcct acaccctctg cttcaacgcc accagcaggt gacaaaactc gaccggatgc
    44701 gaaataaacg gcgcatgggc cgctttggcg aagatatatg attcgctgtg aggccaaagt
    44761 ttatccagca tcggcaccac tttgcgcggc accagaccgt cgagatagcc atacaatcgc
    44821 aaaaacggca tgggcacgtt ttgcagcggc tgacggagat cgaccgtttt caggatttcc
    44881 agcccgccat taagcacgtc aacctccggc atcggtaacg ccagaacggt tttcttcaac
    44941 gcccgcgcat cctggcgcgc cgtttcagtc cccatggttt gtaacgccag gaaccgctcc
    45001 actgtacgct gaaaatcatc actgagttgc tgctgaaatc ccgccagcac gtccggtttt
    45061 atccccggcc actcgtcacg agcactaaaa caaggtgacg acgccacggt gaccagcgcc
    45121 tgaacacgct cgggatgggt taacgcaatc tggcttgcca ccagcccgcc cagactccag
    45181 cctaaccaaa tggctttatc aggtgcctgt tgcagcacgg cttcggccat atcagcaagt
    45241 gacagcgcac caaatccccg gctacgcccg aagccgggca ggtcaacaag gtgcagcgta
    45301 aaatgcgagc taagttcctc gtcaatgcaa cgccacactt cggcattcag tccccatccg
    45361 tgcagcagca caagatgaac attcccctga cctttggtct gccaccagat gttattcatc
    45421 cgctattgtt ctcttttgac ttacaaggat gaacatatgc taacagtacc gggattatgc
    45481 tggctatgcc gaatgccact ggcgttaggt cattggggga tttgttcggt ctgctcacgc
    45541 gctgcccgta cagataaaac gttatgtcca caatgtggat tacccgccac acactcacat
    45601 cttccctgcg gtcgctgcct gcaaaaacca ccgccctggc aaagactggt cacggttgcc
    45661 gactatgcgc cgccgttaag tccgcttatc caccagctta aattttcccg gcgcagtgaa
    45721 atcgccagcg ccctgtcacg tctgttacta ttggaagtct tacacgctcg tcgcaccacc
    45781 gggttgcaat tgccggatcg catcatcagc gttccgttat ggcagcggcg tcactggcgt
    45841 cggggattta atcagagcga tttgctgtgt cagccgttat cacgctggtt gcactgccaa
    45901 tgggatagcg aagccgtcac acgtacacgg gccactgcga cccagcattt tctcagtgcc
    45961 cggctgcgca agcgcaacct gaaaaatgcc tttcgtcttg aattgcccgt gcaaggtcgc
    46021 catatggtga ttgtggatga tgtcgttacc accggaagta ccgtcgcaga gattgcgcag
    46081 ttgcttttac gcaatggtgc ggcgactgtc caggtctggt gcctttgtcg aaccttgtag
    46141 agcctcgatg atgggcgtat tataaccaac taaaatagtc aactattagg ccattactat
    46201 gatccgtatt tccgatgctg cacaagcgca ctttgccaaa ctgctggcaa atcaggaaga
    46261 agggacacaa atccgcgtat ttgtgattaa ccctggcacg cctaacgctg aatgtggtgt
    46321 ttcttattgt ccgccggatg ccgtggaagc caccgacact gccctgaaat ttgacctgct
    46381 gaccgcgtat gttgatgagt taagcgcacc atacctggaa gatgcagaga tcgactttgt
    46441 taccgaccag ttgggttctc agttaacgct gaaagccccg aacgccaaaa tgcgtaaagt
    46501 ggcagacgat gcaccgctga tggagcgcgt ggagtatatg ctgcagtcgc agatcaaccc
    46561 acagcttgct ggtcacggtg gtcgcgtttc gctgatggaa atcacagaag acggttacgc
    46621 cattctgcaa tttggcggcg gctgtaacgg ttgttccatg gtcgatgtga cgctgaaaga
    46681 agggatcgag aagcagctgc tgaacgaatt cccggagctg aaaggtgtgc gcgatctcac
    46741 cgaacaccag cgcggcgaac actcctacta ctaagttatc ccctcttttg tagattgccc
    46801 gatgcgacgc taaagcgtca tttcgggcct acgttgaaca tgtgccagac gttggcacag
    46861 cattgctttg gcaaatccct cccttcgttt ccccgcgata atatgaccaa cctctcataa
    46921 tttaaattta ccccgctctg gtgattctca aacgccagat gttacccgta tcattcacat
    46981 gggtaccaaa catactcctg acatctgact acaataatta gttttagtgg gtatcagtcg
    47041 tggtgccgca atatctctgt tcccgattgg gataattaga gtttgtcgtc agaaaattga
    47101 cgttacccat aacaaatgaa aggccaggta aatcatgcca ttagtcattg ttgctatcgg
    47161 tgtaatcttg ttgttgctcc tgatgatccg cttcaaaatg aacggcttca tcgctctcgt
    47221 cctcgtggcg cttgctgttg gattaatgca aggaatgccg ctggataaag ttattggctc
    47281 catcaaagcc ggtgtcggcg ggacgctcgg tagccttgcc ctgatcatgg gttttggcgc
    47341 aatgctgggc aaaatgctgg cagactgcgg tggcgcacaa cgtatcgcca ccacgctgat
    47401 tgccaaattt ggtaaaaaac acatccagtg ggcggtggta ctgaccggtt ttaccgttgg
    47461 ttttgccctg ttctatgaag tgggctttgt gctgatgctg ccgctggtgt ttaccatcgc
    47521 ggcttctgcg aatattccac tgttgtatgt tggtgtacca atggcagctg cactgtctgt
    47581 gacccacggc ttcctgccac cgcatccggg tccgactgcg attgccacca ttttcaatgc
    47641 cgatatgggt aaaaccctgc tgtacggtac tattctggca atcccgaccg tgattctcgc
    47701 cggtccggtt tacgctcgcg tgttgaaagg tatcgataag ccaattccgg aaggtctcta
    47761 cagcgcgaaa accttcagcg aagaagagat gccgagcttt ggcgtcagcg tctggacttc
    47821 tctggtgccg gttgtgctga tggcgatgcg tgcgattgcc gaaatgatcc tgccgaaagg
    47881 tcacgctttc ctgccggtag cggagttcct cggtgacccg gtaatggcaa cgctgattgc
    47941 cgtgctgatt gcgatgttca cctttggtct gaaccgtggt cgttcaatgg atcagattaa
    48001 cgacacgctg gtttcttcca tcaaaatcat tgcgatgatg ctgttgatca tcggtggtgg
    48061 cggtgcgttc aagcaggtgc tggtagacag cggcgtggac aaatacattg cttccatgat
    48121 gcacgaaacc aacatttctc cgctgctgat ggcctggtcg attgctgccg tactgcgtat
    48181 cgcgctgggt tccgcaaccg ttgcggcaat cactgcgggt ggtatcgcgg caccgctgat
    48241 tgcaacgacg ggtgttagcc cagagctgat ggttattgcg gtgggttccg gtagtgtgat
    48301 tttctctcat gtgaacgatc cgggcttctg gctgttcaaa gagtacttta acctgactat
    48361 cggcgagacc atcaaatcct ggtcgatgct ggaaaccatt atctcggtgt gcggtctggt
    48421 aggctgtctg ctgctgaata tggtgatttg ataacactga ctgccggatg catcgcatcc
    48481 ggcatcaacc gcactctact tcttcttcgc tgcagctctg cgccgtctgt ccaaatcctt
    48541 cagcaacttg ttcacgccat catcggcaaa catcgtctca agcgttgcgg aaagcttgcg
    48601 tcgccagttt ttatactggt aactggtgcc aggaatattc accggttcgg ccatatccag
    48661 ccagtcttcc ggctgtagtc ctaacagagc actgttactg tcggcaatgt agcgctgcaa
    48721 accacggttc agcgtcggcg tcatcgacat caacgatgcc ttatgcccgg cacgttttgg
    48781 cagacaacca tatttatgca gtgcatccag cagcccttgc ttcgccagtt cgcgatcctg
    48841 atacagaccg cgcagtacca cttcatccgg atacagcccc agggttttgc ccagcgttag
    48901 atccccgctc tcccagtaac cgcgtagcgt aggcaggtca tgtgtcgccg caaccgccat
    48961 cgactgctcc ggatacgctt tcggtgcacg gaacgtcttc tcgtggtcgt tttcgaaata
    49021 gagcactttg taggagtaca caccgctgct gcgcagctta ccgacaatct ctaccggtac
    49081 ggtaccgaga tcttcaccaa tcaccataca gcgatgacgt ttactttcga gtgccagaat
    49141 cgagagcaga tcatccaccg gatagtgaac atacgcgccc tgatctgccg tctcgccata
    49201 cggtatccac cacaaacgca gcatcgacat cacatggtca attcgtaatg cgccgcagtt
    49261 ttgcatattg gcacgcaaca gctcgataaa cggttcatag gcacgcgcgg tgatgatatg
    49321 cgggtccatt ggcggtaatc cccagttctg ccccaacggg ccgaggatat ccggcggcgc
    49381 gccaaccgac gctttcaggc aatatagttc acggtcacac caggtttccg ccccaccttc
    49441 cgctacgcca accgccagat cacgatacaa gccaatcggc atttcatagc cctggcttat
    49501 ctcccagcag gcggcaaact ggctgtaagc cagccactgc aaccagagat aaaaatcgac
    49561 gtcattacga tgttcttcgc agaactgacg cacttctggt gaatccacgt tctgatacat
    49621 ctctggccat gcaggccagc cccagcgcat ttcgtcctct ttcacttgct gggcatgtag
    49681 cgcatcaaag gctgcctgcc agaacaggct gtcgccctgc tctgcaacaa actggcgaaa
    49741 cgcggtcatc tgctcatcat cacgttgcgc gaaacctttc cacgccattc gtaatgctgt
    49801 catttttagg gcggtaaccg tggagtaatc gacccagtcg gcatcgcgcg cctgttgcag
    49861 cgtctgttgc gtggtcggca actgccacca ggcctgagcc tcttcgctaa gatggaaatc
    49921 ttcaacggcg ttaacgtcga tataaatcac attcagccaa cggcgagaag acgggctgta
    49981 tgggctggca ctctccggat ttgccggata gagcgcatga atcgggttca ggccgatgaa
    50041 cgacccgcca cgttttgcca catccaccag catcgctttg agatcgccaa aatccccaat
    50101 accccagttt ttttccgatc gcagcgtata aagctgaacg caggcacccc acagcttttg
    50161 tttattcagc aacgcctgcg gttcgtaaca gcgtttcggg gcgacaatca cccggcaatg
    50221 cgcgcgctgg tcgtcctggg tgagtgtcag cgtgtgataa ccttccggca gcttcgtcgg
    50281 tagattgaac gctttgcccc ccgttacatg gcctttgtac tgtgttcctt cttcggtggt
    50341 cagcagccag ctatattcgc cgctgccctc caccaccatc ggcatttttt tgccgctggt
    50401 ataaaccatg acattcggga ctggcgttac cgccactttc gtggcggtac gttgatgcat
    50461 cgcgtcaagc aaacgccgtt tggtttcggc gctaatcgac tgcggtttac cgtgggcatt
    50521 gatgtaattg gggctaatcc ccgccgccag cgcggcatta tccagacgtt tgctttccat
    50581 cgagcttcct tagcgttttg cctgccagat acgagcctga taatcgcgaa tagagcgatc
    50641 cgagctaaac ataccgcagc gggcggtatt gaggatcgcc gcgcgagtcc aggcctcctg
    50701 gtcgcggtac agcacatcca cctgcttttg tgcctcaaca taggctgcga aatccgccat
    50761 caccagatac ggatcgccgc cttgtttgcc gatactgtgc agcatctggt cgaaggcatg
    50821 cttatcgccg tcgctgtatt taccgctttc cagctctttc aatactgcgt ccagcacctt
    50881 atctttcttc cgccatttca ccgggtcgta gcctttggcc agaatagcct tcacttgttc
    50941 cacggtatga ccaaaaataa agatattttc ttcaccgact ttctcggcga tttcaacgtt
    51001 cgcaccatcc agcgtaccga cagtaagcgc accattgagc gccagtttca tattgccggt
    51061 accggaagct tctttacctg cagtcgaaat ttgttcggag atatccgccg ccgggatcag
    51121 tttttccgcc gccgaaacgc aataatccgg caggaacacc accttcaact tatcgccaac
    51181 cagtggatcg ttgttgatca cgtcagccac tttgttgatc gcaaagataa tattcttcgc
    51241 caggtagtag cccggtgccg ctttcgcgcc gaagaggaag acgcgcggta cgcgatcagc
    51301 ctgcgggttt tcacgaattt ctttgtacaa cgccagaata tgcagcagat tcaggtgctg
    51361 gcgtttgtac tcatgcagac gtttgatctg aatatcgaaa atcgcctgtg gattgatctc
    51421 aataccggta cgaactttca caaactccgc cagacggact ttattcgcct gcttgatctc
    51481 gcgatattgc tgacggaatt tcgcatcatc agcgaatttt tccagattga tcagctgatc
    51541 gagatcgtta gcccactctt tttgcagtga tttatccaac agagccgcca gtgcagggtt
    51601 gcactgtttg atccagcgac gtggggtaat accgttggtg acgttatgga atttgttcgg
    51661 ccatagctgg tgatattccg ggaacagatc tttcaccacc agatccgagt gcagcgccgc
    51721 aacaccgttc accgcgaaac cgccaaccac acacaggttc gccatatgca cttgtttatc
    51781 gtgcaccacc gccagtttgg cccacacttt ttcatcgccc ggccaggttt tctccaccag
    51841 cgttttaaag cgagtattaa tttcgttaat aatctgcata tggcgcggca gtaagccttt
    51901 caccagtttc acatcccagc gttccagcgc ttctggcatc agggtatggt tggtgtaagc
    51961 gaaagttttg ctggtaatgg cccaggcgtc atcccagctc atctggtgct catcgatcag
    52021 cacgcgcagc agttctggaa tcgcgatagt tgggtgggta tcgttcagct gaataacttc
    52081 gtaatccgcc agttcgtgca gtttacgccc cgccagatga tggcgacgca aaatatccgc
    52141 taccgaacag gcacactgga agtactgctg catcaagcgc agctttttac cggcagtatg
    52201 gttgtcgttt ggatagagaa ctttggtcag tttttccgca ttgatgccct gctgttcggc
    52261 acgcaggaaa tcaccgtcgt taaatttagt cagatcaaac ggatgcgcgt gcgtcgcctg
    52321 ccacagacgc agcggctgcg ccacgccatt acgatagccg acaacgggga gatcccacgc
    52381 ttgaccggta atggtaaact ccggctccca gcgtccgtct ttcgtcactt taccgccaat
    52441 ccctacctgc acatccagtg cttcgttgtg gcggaaccac gggtagttac tgcgatgcca
    52501 gtcatccggc gcttcaacct gtttgccatc gacaaaagac tggcggaaca aaccatattg
    52561 atagttcaga ccgtaacccg tcgcagactg accgacagtt gccattgagt cgaggaagca
    52621 cgccgccaga cgtcccagac caccgttacc cagcgccggg tcgatctctt cttccagcag
    52681 gtccgtcaga ttgatgtcat aagccttcaa cgaatcctgt acatcctgat accagccgag
    52741 attcaacagg ttgttgcccg tcaggcgacc aatcaaaaac tccattgaga tgtagttaac
    52801 atgtcgctga ttcgccaccg gcttggcgaa tggctgagca cgcagcattt cggccagtgc
    52861 ttcactcact gctagccacc actggcgagg agtcatttca gccgcagaat ttaagccata
    52921 acgctgccac tgacgtgaaa gcgcttcctg aaattgctta tcgttaaaaa taggttgtga
    52981 cataggagtt ccacttttct tagattttca acacaacgtt atcgctagtt tgccaggctc
    53041 gatgttgacc ttcctcatcc tgcgggggat taggcaggga ggagttgcgg ggatgagcaa
    53101 ggaaatgtga tctcaaccac ttaaagctag tgcaaaccac aggattagca gcaaatcaat
    53161 gcaatacagc gcagaaaatc tgtatctaag tgcaaaaaat ggccgttgcg tattttcaaa
    53221 aagcggaagg taactctata aattaagtaa aggagtgaaa cagtttcata agtaaaatat
    53281 ccagtgtgct ccatctcatt cttaatagat ttattaagat catcttttta gatggcactt
    53341 tcatcaggaa tgaagaagaa acccttgctt aaatgaatct gatgaacata agggaaacca
    53401 gtattcacgc tggatcagcg tcgttttagg tgagttgtta ataaagattt ggaattgtga
    53461 cacagtgcaa attcagacac ataaaaaaac gtcatcgctt gcattagaaa ggtttctggc
    53521 cgaccttata accattaatt acgaagcgca aaaaaaataa tatttcctca ttttccacag
    53581 tgaagtgatt aactatgctg attccgtcaa aactaagtcg tccggttcga ctcgaccata
    53641 ccgtggttcg tgagcgcctg ctggctaaac tttccggcgc gaacaacttc cggctggcgc
    53701 tgatcacgag tcctgcgggc tacggaaaga ccaccctcat ttcccagtgg gcggcaggca
    53761 aaaacgatat cggctggtac tcgctggatg aaggtgataa ccagcaagag cgtttcgcca
    53821 gctatctcat tgccgccgtg cagcaggcaa ccaacggtca ctgtgcgata tgtgagacga
    53881 tggcgcaaaa acggcaatat gccagcctga cgtcactctt cgcccagctt ttcattgagc
    53941 tggcggaatg gcatagccca ctttatctgg tcatcgatga ctatcatctg atcactaatc
    54001 cagtgatcca cgagtcaatg cgcttcttta ttcgccatca accagaaaat ctcaccctgg
    54061 tggtgttgtc acgcaacctt ccgcaactgg gcattgccaa tctgcgtgtt cgtgaccaac
    54121 tgctggaaat tggcagtcag caactggcat ttacccatca ggaagcgaag cagttttttg
    54181 attgccgtct gtcatcgccg attgaagccg cagaaagcag tcggatttgc gatgacgttt
    54241 ccggttgggc gacggcacta cagctaatcg ccctctccgc ccggcagaat acccactcag
    54301 cccataagtc ggcacgccgc ctggcgggaa tcaatgccag ccatctttcg gattatctgg
    54361 tcgatgaggt tttggataac gtcgatctcg caacgcgcca ttttctgttg aaaagcgcca
    54421 ttttgcgctc aatgaacgat gccctcatca cccgtgtgac cggcgaagaa aacgggcaaa
    54481 tgcgtctcga agagattgag cgtcaggggc tgtttttaca gcggatggat gataccggcg
    54541 agtggttctg ctatcacccg ctgtttggca acttcctgcg ccagcgctgc cagtgggaac
    54601 tggcggcgga gctgccggaa atccaccgtg ccgccgcaga aagctggatg gcccagggat
    54661 ttcccagcga agcgattcat catgcgctgg cggcaggcga tgcgctgatg ctgcgcgata
    54721 ttctgcttaa tcacgcctgg agtttgttca accatagcga actgtcgctg ctggaagagt
    54781 cgcttaaggc cctgccgtgg gacagtttgc tggaaaatcc gcagttagtg ttattgcagg
    54841 cgtggctaat gcaaagccaa catcgctatg gcgaagttaa caccctgcta gcccgtgctg
    54901 aacatgaaat caaggacatc agagaaggca ccatgcacgc agaatttaac gctctgcgcg
    54961 cccaggtggc gattaacgat ggtaatccgg atgaagcgga acggctggca aaactggcac
    55021 tggaagagct gccgccgggc tggttctata gccgcattgt ggcaacctcg gtgctgggtg
    55081 aagtgctgca ctgcaaaggc gaattgaccc gctcactggc gctaatgcag caaaccgaac
    55141 agatggcacg ccagcacgac gtctggcact acgccttatg gagtttaatc cagcaaagtg
    55201 aaattctgtt tgcccaaggg ttcctgcaaa ccgcgtggga aacacaggaa aaagcgttcc
    55261 agctgatcaa cgagcagcat ctggaacagc tgccgatgca tgagtttctg gtgcgcatcc
    55321 gagcacaact gttatgggcc tgggcacggc tggatgaagc cgaagcgtcg gcgcgtagcg
    55381 ggattgaagt cttgtcgtct tatcagccac agcaacagct tcagtgcctg gcaatgttga
    55441 ttcaatgctc gctggcccgt ggtgatttag ataacgcccg tagccagctg aaccgtctgg
    55501 aaaacctgct ggggaatggc aaatatcaca gcgactggat ctctaacgcc aacaaagtcc
    55561 gggtgattta ctggcaaatg accggcgata aagccgccgc tgccaactgg ttgcgtcata
    55621 cggctaaacc ggagtttgcg aacaaccact tcctgcaagg tcaatggcgc aacattgccc
    55681 gcgcacaaat cttgctgggc gagtttgaac ctgcggaaat tgttctcgaa gaactcaatg
    55741 aaaatgcccg cagtctgcgg ttgatgagcg atctcaaccg taacctgttg ctgcttaatc
    55801 aactgtactg gcaggccgga cgtaaaagtg acgcccagcg cgtgttgctg gacgcattaa
    55861 aactggcgaa tcgcaccgga tttatcagcc attttgtcat cgaaggcgaa gcgatggcgc
    55921 aacaactgcg ccagctgatt caactcaata cgctgccgga actggaacag catcgcgcac
    55981 agcgtattct gcgagaaatc aatcaacatc atcggcataa attcgcccat ttcgatgaga
    56041 atttcgttga acgtctgcta aatcatccgg aagtgccaga actgatccgc accagcccgt
    56101 tgacccaacg tgaatggcag gtcctggggc tgatttactc cggttacagc aatgaacaaa
    56161 ttgcgggcga actggaagtg gcggcaacca ccatcaagac gcatatccgc aatctgtacc
    56221 aaaaactcgg cgtcgcccac cgtcaggctg ccgtgcagca cgcgcagaaa ctgctgaaga
    56281 tgatgggtta tggggtgtga gtttagccgg ataatgcatt ggatccggtt tacatcttta
    56341 catcattcaa tactcacccg cgttacgcca tctgtttcta tcaaaccaaa ccgcaccggc
    56401 aagaaacgct ccaccaccgc gatattggtc agcagatggc atgaaggatg ggcgacctta
    56461 aattctcccg cgcccgccag cgccatcggc agtaccagtt ggtcggcgag atattccccc
    56521 accgccgctg ggcttgccag gtagcgtttc acctctttca ctaactgcgc ggctaccacc
    56581 tcggcactga cgcgcttttc accgacgacg aaaaagcgtt cagtgatatt ttcactttcg
    56641 acttccagcg agacggtatt acccggcccc tggtcgcgcg gcaggttatg aatattctgt
    56701 tcatgcaggg aaaaactccc cgccagtgta gcgatttcac gctcagcaac atggcgcggc
    56761 acaccagcta atagaacttc tccacgcatc tgcacaatgt tcccgcgctc gccaagttgc
    56821 aaggtgttaa acgatgccac gggcgagact tccgttgcca ccacgccgcc tccggcagga
    56881 taaaaaccgt gacgtaacag cgtggtttgc tgatgaattc ctattttcgc cagcagcggc
    56941 tccagcaccc ggcggataaa atccgcaggc ggggccgacg ggttatcggt gcctccgctc
    57001 acttcaacac gcgaaggtcc atcggcaaac cacagcgcgg gcagcaccgt ttgcagcacc
    57061 agcgtacagc ttccggcact accgatagca aagcggtact cgccgccgcg cacggtgcca
    57121 ggccggaaga ccagacgctg cgaccccagc tccgcgcctt ccaccgttgc cctacaaatt
    57181 tccgcagccg cttttaccgc ggtcagatgc tggcgcaaca gccccggttt cgctcgcccg
    57241 gcacgaatgc tggtgatggt aaatggctgg ccggttatca tcgacaggct cagcgccgag
    57301 cgcaggatct gcccgccacc ttcgccctgt gcgccatcca gcgcaatcat ccttttcatc
    57361 atttatcctt ttacgcacac cacctgacgc agggtataga taacttccac cagatcgctt
    57421 tgtgccgcca tcaccgcatc aatatcttta tacgccatcg ggatttcgtc gatcacttcg
    57481 gcatctttac ggcattccac atgcgcggtg gcacgaattt gatcttccac gctgaacagt
    57541 tttttcgctt tagtacggct cattacccgc ccggcaccgt ggctgcacga acagaacgac
    57601 tcttcatttc ccagcccacg gacgataaag cttttcgctc ccatcgaacc gggaataatt
    57661 ccatattgac cagcacgcgc agacaccgcg cctttacgcg tcacgtagat ctcttcgcca
    57721 aagtgctgtt ctttttgcac atagttgtgg tgacagttga tctcttccat cgccagcgtt
    57781 tgctgttgtc ttaccgtttt ctgcgtaacg ctctgcaacg ctgttactac gttttccatc
    57841 attgcatcgc ggttaaggct ggcaaaaagc tgcgcccagg ccaccgcttt caggtaatca
    57901 tcaaagtatt ccgtaccttc cataaagtac gccagatcac gcgacggcaa cgtttcaagc
    57961 tggtcctgca tctctttttg cgccagatcg ataaagtaag tcccgatggc gttaccaatt
    58021 ccgcgtgaac cggagtgcag cataatccac acctgatccg actcatcaag acagatttca
    58081 ataaaatggt tacccgttcc cagcgttccc agatgtttat agttattggt attcaggaaa
    58141 cgcggatatt tttgcgttaa ccactgataa ccggcttcca gctcagccca tttagcatcg
    58201 acgttaacag gtggattttc ccaggcacct ttatcacgtt tacaacggcc agtggtacgc
    58261 ccgtgcggca cggccgtttc aatcgcctga cgcagctctg ccaggttttc aggcaggtct
    58321 tccgccgtta acgcggtacg cagcgcgttc attccacagc caatatccac gcccaccgcc
    58381 gccggaataa tcgccccttt ggtcgggatc acgctaccaa tggtggaacc tttacccagg
    58441 tgtacatcag gcattaccgc aatatgtttg aaaataaacg gcatcttcgc cgtattaata
    58501 agttgctgac gcgcatcggc ctctaccggc acgcctttgg tccacatttt taccggggca
    58561 ttttcagtgg tcagtaattc gtaattcatt ttgtttttct cttttcgttg tttgctgtcc
    58621 tgataattgc aaccgtcgtg ccagaaaatt aaataagcag cttaattttt taattcattg
    58681 ttttaaaaaa gattatatct ttacgtccgt acccggagat ttcccgcaaa gccaatttac
    58741 cgataatgaa atatcgtctt ttataaggat atctaagatg cgtaaaacag tggcttttgg
    58801 ctttgtcggt accgtactgg attatgccgg gcgcggcagt cagcgctggt caaaatggcg
    58861 tccgacactc tgtttatgcc agcaagaatc gttggtcatc gatcgactgg aattgttgca
    58921 cgacgcccgc tcgcgctcgc tatttgaaac gcttaaacgc gatatcgcca gcgtttcgcc
    58981 agaaacagaa gtggtgagcg ttgagattga actgcataac ccgtgggatt tcgaagaggt
    59041 ctacgcctgc ctgcatgatt tcgcccgtgg ttacgagttt cagccagaaa aagaagacta
    59101 tttaattcac atcaccaccg gtacccacgt cgcgcagatt tgctggtttc tgctggcaga
    59161 agcgcgttac ctgcccgccc ggctgataca atcttcacct ccgcgcaaaa aagaacagcc
    59221 gcgcggcgca ggtgaagtga cgattatcga tctcgattta agccgttata acgccatcgc
    59281 cagccgcttt gccgaggaac gccagcaaac gcttgatttt cttaagtccg gcattgccac
    59341 gcgtaccccc cacttcaacc gcatgattga gcagatcgaa aaagtggcga tcaaatcccg
    59401 cgcgccgatt ctgcttaacg gtccaaccgg cgcgggcaag tcatttctgg cgcgacgcat
    59461 cttcgagtta aaacaggcgc ggcatcagtt tagcggcgcg tttgtggaag tgaactgcgc
    59521 caccctgcgc ggcgataccg ccatgtcgac gctgtttggt catgtaaaag gcgcgtttac
    59581 cggggcgcgg gaatctcgtg aaggtttatt acgcagcgcc aacggcggaa tgttgtttct
    59641 tgatgagatt ggcgaactgg gcgcagacga acaagcaatg ctgctaaaag ccattgaaga
    59701 gaaaaccttt tacccgtttg gcagcgatcg ccaggtgagc agtgattttc agcttatcgc
    59761 cggaacggtg cgcgatttgc gccagctggt tgccgaaggc aaatttcgcg aagatctgta
    59821 cgcgcggatc aatctctgga ccttcaccct gccgggtcta cgccagcgcc aggaagatat
    59881 tgaaccgaac cttgattatg aagtggagcg ccacgccaca ctcactggcg acagcgtgcg
    59941 ttttaacacc gaagcgcggc gcgcctggtt ggcttttgcg acctctcccc aggcaacatg
    60001 gcgcggtaac tttcgcgaac tttctgccag cgtcacgcgg atggccacct ttgccactag
    60061 cggacgcatc actctggacg tggttgaaga tgagataaac cgtctgcgct ataactggca
    60121 ggagagccgc ccctccgcgc ttacggcgtt gctgggcgca gaggcagaaa acatcgatct
    60181 cttcgaccgt atgcaactgg aacacgtgat agctctctgt cgccaggcaa agtcgctttc
    60241 cgctgccgga cgcctgcttt ttgatgtttc gcgccagggc aaagccagcg tcaatgacgc
    60301 ggatcggcta cgcaaatacc tggcgcgttt tggtctgacg tgggaagccg tgcaggatca
    60361 gcacagctcc agttgaatat gatggtccgt cagcacctgc attacgctta ctggcggcgg
    60421 ggcgtcggtg tagacggcat ctaccatgct gatgctgccc atattgacca tcgcgttacg
    60481 gccaaatttc gagtgatcga caaccagcat aacgtggcgc gagttctcaa taatggcgcg
    60541 cttggtgcga acttcgtggt aatcgaactc cagcagcgag ccgtcgctat cgatgccgct
    60601 tatccccaga atgccgaaat caaggcggaa ctgggagata aaatcgagcg tcgcttcgcc
    60661 aatgatcccg ccatcgcggc tgcgtaattc gccaccggcg agaataatgc ggaaatcttc
    60721 ttttaccatc aacgtgttag caacattgag attgttggtg acaatacgca aattgctgtg
    60781 attaagcagc gcgtgcgcca ccgcttccgg cgtggtgccg atatcgataa acagtgtcga
    60841 gccgttgggg atttgctccg ccactttgcg ggcaatcctc tctttttctt cggtctgggt
    60901 ggctttgcga tcgtgccacg gcgtgttaac cgaactggaa ggcagcgccg caccgccatg
    60961 gtgacgcagg atcaggtttt gctccgccag ctcattgagg tcgcggcgaa tagtctgcgg
    61021 gctgacggag aaatgctcta ccagctcttc agtactgaca taaccctgct gtttaaccag
    61081 ttcgataata ccgttgtgac gttgtgtttg tttcatttat aaatccctgg aattattttc
    61141 gttttcgcgc attgagcgaa tcaacaaaag ccatcgctaa acccacggct aacccggcga
    61201 tgtgtgctcc gttcgccatc gacatcccaa acaaatcaaa ccatccggcg acaatccaga
    61261 tcagcgcaaa gataattaac ccacgttgca ggtaaatgcc actttgcgga tcgcgttcac
    61321 cacgtagcca gacgtagccc atcagcgcat acaccacgcc agaaagcccg ccaaaccacg
    61381 gcccgctgaa tttttgctgc acatagccgc ttaacagggc gctaatgaga gtaataacaa
    61441 ttagcttacc gctaccgagg cgtttttcca ccgcaccgcc gagataccac caccagagca
    61501 ggttaaagag gatatgcatc agcgagaagt gcattaacgc gtgggtgaag taacgccaga
    61561 actcaaattt cagtgttgga tcgaatggcc aggccagcca taacatcact tcctgatcgc
    61621 cgagaatttg catggcgata aacaccacca cgcaggcgat catcaccacc caggttaccg
    61681 gacctgcgcg ttcacgcaag gcagcaaaga aaggataacg gcgataatgc agaccactgc
    61741 cggtatggcc cgcctgccag ctggccgcca gataacgcgg atctgccggg ttttcgagaa
    61801 aacgcgccag ctccgcccgc acgcgctcgg cctgggactc atccgccagc cagacatcgc
    61861 tttggttatg ttgttgaatc gtaaggataa caccctgcgt cgccatgtaa tcaacaaacg
    61921 cctgcgccac gcgggggtta gcaaaagagg taatcatcaa catcgttgct gtcgcttatt
    61981 ccacacaaaa ggggacagta taaagcgtta cgcgccgtac gccacctctg cgggaaactg
    62041 acgctgccag gcttcaaaac cgccgtcaat gctatagacc acatcgtagc cctgttgcag
    62101 cagatactgc gccgcgcctt tgctgctatt gccgtgataa cacatcacca tcaccggagt
    62161 gtcaaagtcg ttatcacgca taaacgcgcc cagcgtgtcg ttggttaaat ggaaagcctg
    62221 caccgcatgc cccattgcga aactctgtgg atcgcgaata tcgaccagca ccgcctcttt
    62281 ttcctgcaac ttctggtgcg cgacggcaac gttaatacat tcgaactgat ccatgcgtct
    62341 ctctttcttt acaaacaagt gggcaaattt accgaacagt ttacgtcgaa gcggcagata
    62401 aacgccataa tgttatacat atcactctaa aatgtttttt caatgttacc taaagcgcga
    62461 ttctttgcta atatgttcga taacgaacat ttatgagctt taacgaaagt gaatgagggc
    62521 agcatggaaa ccaaagatct gattgtgata gggggcggca tcaatggtgc tggtatcgcg
    62581 gcagacgccg ctggacgcgg tttatccgtg ctgatgctgg aggcgcagga tctcgcttgc
    62641 gcgacctctt ccgccagttc aaaactcatt cacggtggcc tgcgctacct tgagcactat
    62701 gaattccgcc tggtcagcga ggcgctggct gaacgtgaag tgctgctgaa aatggctccg
    62761 catatcgcct tcccgatgcg ttttcgcctg ccacatcgtc cgcatctgcg cccggcgtgg
    62821 atgattcgca ttggtctgtt tatgtacgat catctgggta aacgcaccag cctgccggga
    62881 tcaactggtt tgcgttttgg cgcaaattca gtgttaaaac cggaaattaa gcgcggattc
    62941 gaatattctg actgttgggt agacgacgcc cgtctggtac tcgccaacgc ccagatggtg
    63001 gtgcgtaaag gcggcgaagt gcttactcgg actcgcgcca cctctgctcg ccgcgaaaac
    63061 ggcctgtgga ttgtggaagc ggaagatatc gataccggca aaaaatatag ctggcaagcg
    63121 cgcggcctgg ttaacgccac cggcccgtgg gtgaaacagt tcttcgacga ggggatgcat
    63181 ctgccttcgc cttatggcat tcgcctgatc aaaggcagcc atattgtggt gccgcgtgtg
    63241 catacccaga agcaggccta cattctgcaa aacgaagata aacgtattgt gttcgtgatc
    63301 ccgtggatgg acgagttttc catcatcggc accaccgatg tggaatacaa aggcgatccg
    63361 aaagcggtga agattgaaga gagtgaaatc aattacctgc tgaaagtgta taacacgcac
    63421 tttaaaaagc agttaagccg tgacgatatc gtctggacct actccggtgt gcgtccgctg
    63481 tgtgatgatg agtccgactc gccgcaggct attacccgtg attacaccct tgatatccat
    63541 gatgaaaacg gcaaagcacc gctgctgtcg gtattcggcg gtaagttgac cacctaccgc
    63601 aaactggcgg aacatgcgct ggaaaaactg acgccgtatt atcagggcat tggcccggcg
    63661 tggacgaaag agagcgtgct gccgggtggt gccattgaag gcgaccgcga cgattatgcc
    63721 gctcgcctgc gccgccgcta tccgttcctg actgaatcgc tggcgcgtca ttacgctcgc
    63781 acttacggca gcaacagcga gctgctgctc ggcaatgcgg gaacggtaag cgatctcggg
    63841 gaagatttcg gtcatgagtt ctacgaagcg gagctgaaat acctggtgga tcacgaatgg
    63901 gtccgccgcg ccgacgacgc cctgtggcgt cgcacaaaac aaggcatgtg gctaaatgcg
    63961 gatcaacaat ctcgtgtgag tcagtggctg gtggagtata cgcagcagaa gttgtcgctg
    64021 gcgtcgtaag gatgatgccc ggttgtgaag tgcaaccggg ctatttggat tccttagact
    64081 attcattgta aataattgaa taacggcccc tgagctaaaa tcgccaaact taagcacgtt
    64141 acccaatcgc cccacttctt cctctggttt ctattttttc tgcaccaatg gcaataatat
    64201 cccaatgcaa aaccaagctg tgcactaatc gggccgacaa aatcagtcca aggctgcggc
    64261 caaaaagggg aaaaattcag gggaagccag acaaacgtca gaaagaaatt tcggatattt
    64321 tccagcaacg ctgatttctc tggagaaata taaagaatga aattcgagag aattaaccag
    64381 aaatagaccg gcgaaaagac cagccagcaa tgcagtctgt tttttaattc aggaggcgtt
    64441 ctgcgggaga attgccaacc gatgccagcc attacactga aaaaaagtgc atattcaacc
    64501 attaactcaa gttgtagttc ctgaagagaa atacgcacaa ccaggagcgg aaaggagaat
    64561 attatttctg ccagaaaagt taacgtgaat ataagtacgg ctaagacaat aaccgtcggc
    64621 cataccaata atggcacgat aacaaaaccg agaattacag ggggaatata aagccggaat
    64681 ttaatcattt ttattttcct ggtgattcgg atgatgcgtc atacatcgcg cataaacact
    64741 catcaattat ccagtcgtct acccagacct gatgctgtgt gccctgttct ggctgctttg
    64801 gtgggttctc aatcgcctgc cgcgcgcgtt ctttcaagat ggattgattg ttgaaggccc
    64861 aggcattgat ttgggtggta atgacgttat cgatatcgtc ctggttatct ctgatataat
    64921 cctcacaata tctgttcaat aaacaggcaa gagaaacggt cgaccaaaag ccgggaactg
    64981 aattaatggc atccagcaat ggcgcaagag cgatatcggc acggaaaaac atttccagca
    65041 tatactgtaa ttcgacagct tcatcgccgg atacccaatc agtcatatac tggcaggcaa
    65101 atcgttgcaa aattttccat tcatcctcac gccaattttc tttactggca aggtttatac
    65161 gccgtagaga aaatacttcg tgaatattgt caaactgccc ctgtgcaaca aattccagta
    65221 aacgcggcag aaaatagcgg acctcatcgc tattttgcgg atcaggccct gggctactat
    65281 tccatgcatt gattaaggaa tacgagatcg ctcgtaacga tgtactccgt aatacctttt
    65341 gttcctgctg actcaggcaa tattcacaac aaaccacaaa tttctgcggc accggataac
    65401 gacgaaaaat atgataaatc tgctcaatta atgcctgcat ggcggggctg ggttgcataa
    65461 ttaccccctc gcccgctgat cgcgtgtaaa cggttgataa acaaaacgag cattcgccac
    65521 gttcaggcga ataatggcac tacgagagag ggaaaagctg gggcgaatgc aactattgca
    65581 gtctcgacag ctttcttcag cgcaattttc cactgtgtaa taacgagcgt agagtttcag
    65641 ttccgcttgc ttatcacgaa atccgcgtaa actgttatcg ttatgaaatt tccaggcatc
    65701 tttaaagacc tgaaaaatgt agatatcctc aatccaggac tcacattttt taagcgggcg
    65761 ctttgctatg atcacccgct catccgggtt tagcatcgcc gtaaattgaa cggtgaggtc
    65821 atttgtcgtg ttcagatcac attgaacatc tggtacatca ctgatatagg ggtcaaatcc
    65881 aaagtcagca ccacaggcac aaagacggct aatggtggcg gtatcgaggg aaaatcccgg
    65941 ccatacattg ctggacgtta agccaatatt taatgtgaca ttatatcgaa gcaatccctc
    66001 aagaccgtca gcaccgacga acttttcccc ttcatccagg gcctggttaa tgagttgctg
    66061 aacaatgcct tcatcccagt atgccgattt gcagttggca atcgtaatat ccacgcctgt
    66121 gaatggatca gtaccgacaa ggcttgcaaa taatgtatta actaattcca tttttattat
    66181 ccgccttaat gcaacgtttt atacatcggc acataaccta catcttccac caggcactgc
    66241 ccctgcggag ttaaaaacca ctccaccaat ttttgtgttt ctgacgtcat attttcccgc
    66301 gttaccataa aggcatcgac gatataggga tatttgccgt tgcgaatgtt ttcggcagtc
    66361 ggtgcaatac cgttaatcgc tagcagtttt atatttttat cggcgttcat ttgtgttgcg
    66421 taataacgga aggtgtaacc tattgcgttg tttgtattac ggtattcggc aacgacttta
    66481 atcattccct ccatcatact tgccacttct gtttcctgcg gcgagatcat gcggacattt
    66541 ttcatgacct gggattgcat caccgtctgg ctaccagagt cttccgggcg ttgccaggtc
    66601 tggatctcct gatcgttacc gccaaccgtg cgccagttgg taattgcacc actgaagatg
    66661 tcacgcactt gttgttcggt cagggaatta accgggttat ctacattgac gatgaaaaca
    66721 aacgcttcac gggcaaaagg cgtgtaaatc aaagtgacgc ccgattcctc cgcgcgtttt
    66781 ttctgcccac cggaaggttg agccacaaag ataatatcgg cattaccttt aacaatcttg
    66841 ttatatgctt cgggagtacg agagttcgcc agatattccc attcgtgaaa atcttccggc
    66901 aaaacgctta gtgcataaaa ggcagaggca taaatggggt acgccgccgt tgcgccatca
    66961 agtcgcggcc agttttgcgt gaactgaatt tgcggtttac cacgcagtgg tgtcagttga
    67021 ttattgagtt tatcgggtcg ccaggcccag gtatcaatat tttcacgcac accgacgccg
    67081 ggattaagct gtttatataa ataagcctgg taaccggcga caaaatcaag catgagaagg
    67141 ataaacaaga gagaattacg gcaacgtaac ccggacgtgc ccgttaaagg atatctggtc
    67201 ggccagccgt accccagcga aaaactgata tgcgcgccaa caggtataat gactaaccac
    67261 cagtactcac caccgactaa tgaggctaac aaaacgccca gccaggggag cataagatca
    67321 gccaaccaac ccgctcccat ctggcgaaat tgataatctg ccagatgcag cgcgattatc
    67381 cagaccccca gggttaataa tgcgggtact aaaaaaagac attgtcgcca gacggctatt
    67441 tctctttttt ctggtgcact atcgtccgcc cacactcgcc ccgagaaaaa tagcattaat
    67501 gcgcccagcg ccagcataaa aatgttgagc agaaggtaaa ttgttggcgt gataaagata
    67561 ttacaaactt caataatacc ggcaagtccg acacccagca tggcaataac cgccgccaaa
    67621 aactgcgcca gaatggggat gccgaaaaaa gtcattacca gcgaggtcaa aatccatttt
    67681 ctgttttgca ttattctttc cattctttct gaatggcgaa attatactcc ccgagtcccc
    67741 ttgccccttc tggacacttt tccgaaatga tggcgaaaaa aacgggaccc tttggccccg
    67801 ttctatttat tagtgaaatt acaatctcac cggatcaata tgccagatat gatcggcgta
    67861 ctctttgata gtacggtcag aagagaagta gcccatattg gcaatgttca gcatcgcttt
    67921 tgcggtccac tcttcctgaa gctcgtagag ttcgtcgact ttatcctgac aatcgacata
    67981 gctgcgataa tccgccagaa cctgatagtg atcgccgaag ttgatcagcg aatcaaccag
    68041 atcgcgatag cgacccggat cttccggact gaatacaccg ctgccgattt gcgtcagcac
    68101 ctgatgcagc tcctcatctt tctcgtagta ttcacgcggt ttgtagccct gacgacgcag
    68161 ttcttccact tcttccgcag tgttaccaaa gataaagata ttgtcagcac cgacatgatc
    68221 cagcatctcg acattcgcac cgtccaacgt accgatagta agcgcaccgt taagcgcaaa
    68281 cttcatgtta ctggtgccgg aagcttccgt ccccgccagc gaaatctgtt cagacagatc
    68341 ggccgccgga atgatcaact gcgccaggct aacgctgtag ttcgggatga acacgacttt
    68401 cagcttgtcg ccaatctgcg gatcgttgtt gatcactttc gctacgtcat tgatcaaatg
    68461 aataatgtgc ttcgccatgt aataggccga agccgcctta ccgccaaaaa tattcacgcg
    68521 cggtacccac ttcgcatccg ggtcggcctt gatgcggtta tagcgggtaa tcacatgcaa
    68581 cacattcatc aattgacgtt tgtattcgtg aatacgtttg atttgtacat cgaacaacgc
    68641 ctttggattc accaccacat tcagctgctg ggcgatatac tctgccagac gctttttgtt
    68701 ctccagcttc gcctgatgca cagcgtgatt aaccattggg aaatcacagt gttgttgcag
    68761 ctcattaagc aggctaaggt cggtgcgcca gttacggccc aggtgttcgt ccagcacggc
    68821 tgaaagcgat gggttcgcta ccgctagcca gcgacgcggc gtcacaccgt tggtgacgtt
    68881 ggtgaaacga cccgggaaga ttttcgcaaa gtcggcaaac aacgattgca ccatcagatt
    68941 agagtgcagt tccgatacac cgttaacttt gtggctcaca acaaccgcca gccaggccat
    69001 acgcacacga cgaccgttgg attcatcaat gatcgacgcc cgtcccagca gatcggtatc
    69061 gttcggatac tgttcctgca aggttttcag gaaatagtcg ttgatttcaa agatgatctg
    69121 caggtgacgc ggcagaattt tacccagcat atcaaccggc caggtttcca gcgcctcgct
    69181 catcagcgtg tggttagtgt aggagaagac ctgacaacac acctcaaacg cgtcgtccca
    69241 gctaaattgg tgctcatcga tcagcagacg catcatctca ggaatcgaca gtaccggatg
    69301 ggtatcattg agatgaatcg cgattttatc cgccaggtta tcgtaggttt tatgcaactg
    69361 ataatggcgg cttaaaatgt cctgaatggt cgaggaaacc aggaagtatt cctgacgcag
    69421 gcgcagctca cgcccggagt aggtggagtc atccggatac agtacgcgag atacgttctc
    69481 ggagtggttt ttatcttcca ctgccgcgaa gtagtcaccc tggttgaatt taccgaggtt
    69541 aatttcgcta ctggcttgcg cactccacaa acgcaacgtg ttggtcgcgt cggtgtcgta
    69601 accagggatt atctgatcgt aagcgactcc cagaatctct tcggtttcaa tccaacgcgt
    69661 ttttttacct tcctgctgaa tgcgaccacc aaaacggact ttatagcgcg tgttgtggcg
    69721 tttgaattcc cacgggttac cgtattccag ccagtagtct ggcgactctt tctggctacc
    69781 gttaacgatg ttctgcttga acataccgta gtcatagcgg atgccgtaac cgcgccccgg
    69841 caaccctaac gtcgccagag aatcaaggaa gcaagccgcc agacgtccca ggccaccgtt
    69901 accgaggcct gggtcatttt cttcatcaat cagctcttcg agatttaacc ccatcgcttc
    69961 cagtgcgccc tgtacatctt cgtaaattcc tagcgacaac atggcgttgg agagcgtacg
    70021 gccaatcaaa aactccatcg acaggtagta aacctgacga gtttcttgcg acaactgggc
    70081 acggtttgaa cgtaaccagc gctccacgag acgatcgcgc acagcaaata acgttgcgtt
    70141 cagccattca tgtttattgg cgacgaccgg gtcctttcca atcgtaaaca tcagcttgta
    70201 agcgatagag tgcttaagag cttctacgct aagcgtgggc gatgaatatg taaacggagc
    70261 attcatatag gcgtttcctg aaaactattt caagcgatag taaagctcac ggtacgactt
    70321 cgccgcgacc tgccagctaa aatccattgc catagcctga cgttgcacaa accgccacag
    70381 tgaaggacgg gaccacagta caaaagcacg tcgaatagcc cgtaacagcg accaggcatt
    70441 actatcttca aagacaaacc cactggcgac gccatctgca aggttctcaa gagaacagtc
    70501 agaaaccgta tcagcaagcc caccggtgcg ccgcactaac ggcagcgtac cgtacttcaa
    70561 tccataaagt tgcgttaagc cgcacggttc aaaacggctg ggcaccagaa tgacatccgc
    70621 gccgcccata atgcgatgcg aaaatgcttc gtgatagcca atctgaacgc ccacctggcc
    70681 ggggtattcc gctgccgccg caaggaaacc ttcctgcagc accggatcgc ccgcgccgag
    70741 tagcgccagc tgcccgccct gctccagaag gcccggtaag gcttccagca ccaggtcgag
    70801 acctttctgg ctggtcagac ggctcaccac tgcaaaaagc ggcactttat catcaacctt
    70861 aagccccatt gcgatttgta actggcgctt attttccgct ttatcttcca acgtatcgcg
    70921 ggtgtagcgc gaggccaaca gtaagtccgt ctctggactc cagattttct cgtccacgcc
    70981 gttcagtacg ccggaaagac gcccttcacg gtgacgctgt tgcaacagac cttccatacc
    71041 gtaggcaaac tgcggttcgg tgatctcgcg agcgtaggtt ggactgaccg ccgtaatgtg
    71101 atcggcatag tacagaccgg ccttcaggaa agagatttgt ccgttgaatt ccagcccatg
    71161 aatattaaag aatgaccatg gcaattggat gtcattcata tgatgtgcat aaaacatgcc
    71221 ttgataggcc aggttgtgca cagtaaacac cgacttcgcc ggacgcccgc gcgccgccag
    71281 atacgcaggc gcaaggcctg catgccagtc gtgcgcatgc accacatcag gacgccagaa
    71341 tgggtcaagc ccgctggcca tttctgcccc aacccacccc agcagcgcaa aacgcaatac
    71401 gttgtcggta taggcaaata agttggtatc gtgatacggg cttcccggac gatcatagag
    71461 atgcggcgcg tcaatcaggt atatgccaac cccgttgtaa tgaccgaaca acagcgtgat
    71521 atgtccggcg aaggtatcac gacgggatac tacctgcgca tcggtaacgc cacggcgaat
    71581 atcgggaaat gcaggcaaca gtacgcgagc gtcaacgccg tctgcgattt gtgctgcggg
    71641 taatgcccca ataacatcag ccagaccgcc ggttttaagc agcgggaaca tctctgaaca
    71701 tacatgtaaa acctgcatta tcgctcctgt ttatgcccta acttccgtag catttcgcgc
    71761 gttaccagca cgatgccttc ttctgaacga tagaaacgac gtgcatcttc ctctgcgttt
    71821 tcaccaatca ccatgccttc cggaataaca caagcacgat cgatgacgca gcggcgcaga
    71881 cggcacgagc gacctaccca tacttccggt aacaatacgg cggaatcaat gttgcagaat
    71941 gaattcacgc gaacgcgcga gaacagaacg gactgcacca ccaccgaacc ggagatcaca
    72001 cagccgccgg ataccagtga gttaagggtc atcccgtggc taccggagcg atcctgcacg
    72061 aatttcgctg gcggtaatga ttcattgtag gtgcgaattg gccaattgcg atcgtacata
    72121 tccagttccg gcaccacaga ggccagatcg aggttcgctt tccagtaagc ttccagcgta
    72181 cccacatcgc gccagtacgg ctcggcatcc gggtcggatt gtacgcaaga gagcgggaac
    72241 gggtgcgcat aggccagacc ggcttcggtg atcttgggaa tcaaatcttt gccaaagtcg
    72301 tggctggagt tctcatcacg atcgtcttct tccagcagtt catacagata gtcggcgtca
    72361 aagacgtaga tacccatact cgccagagat ttgctcggat cgttcggcat tgacggtggg
    72421 ttagcaggtt tttcaacgaa ttcgataatt ttatcgttct catcaaccgc cataacgcca
    72481 aatgcggagg cttcttcaat cggtactggc atacaagcaa cggtgcaacg tgcgcctttt
    72541 tcgacgtgat cgataagcat acgcgagtag tcttgcttgt agatatggtc gcccgccagg
    72601 atcaccacgt attccgcttt ataacggcgg ataatgtcga ggttttgggt gaccgcatct
    72661 gcggtgccgc gataccagtt ttcccctttc attctctgct gtgctggcag cagatcgaca
    72721 aactcgttca tttcttcatt gaagaatgac cagccgcgct gaatgtgctg caccagagtg
    72781 tgggactggt actgggtgat cacgcccata cgacggatcc cggagttgat gcagttagac
    72841 agcgcaaagt cgataatgcg gaacttaccg ccgaagtgta cggccggttt tgctcgctta
    72901 ttggttaaat ccttcaggcg ggtaccacgt cctcccgcca gtatcagggc aacagatttc
    72961 aatggcagct ggcgcgccaa cattaagtga tcgttcttct ctaaactaac catgactaac
    73021 tcctttttta tcatctctgg aacacacaca atccgtgtgc aggtccctgc cagacagccg
    73081 taatcactgg gttatcctct ccagcgaatg ggggaatggc gtgccactcc ccagcaggta
    73141 aaacaatctc tgttacctca agcgtggcgt taattgcgat caaaaagcga tccgagagca
    73201 gaatttgcag ctgtttcggc ccgttttgcc actcatccgt gcttaaaggt tgagcatatc
    73261 gatttagcca acggacattg ccgtcgcctt cttcccacca gcgattctcc accaaagcgg
    73321 gaatgcgctt gcgcagatgg attaacgcgg cggtaaatgc ggttaaacca ctgcttgcct
    73381 gcgaccagtc caaccaggtt aattggttat cctgacagta ggcattgtta ttgccatgct
    73441 ggctgtgacc atgttcgtca ccggccagta acatcggcgt accctgggag agcaacaacg
    73501 ttgttaacag ggcgtgaatg ctgtcgcgcc gccgttcaac caggtcaaga gaaccgccta
    73561 acccttcttt accatgattg ttactgtaat tgttgttggt cccgtcgcga ttttcttctc
    73621 cgtttgcttc attgtgttta tggttgaagc aaacgcagtc gcgaagcgta aaaccgtcat
    73681 gcgcggtgac gagattaatc gcggcactcg gcagacgacc attacgttta aaaacatcgc
    73741 tggaggcagc aaaacgcccg gcaaacgccc ccagaggcaa atcataatgt agccagaaac
    73801 gacgggcagc atcgcggaaa tgatcgttcc actcggcaaa cagcggcggg aaatttccca
    73861 cctgataacc accaggagcg atatcccacg gttcagcaat taacttcacc tgcgagagca
    73921 ccgggcagtt ctggatagcg gtaaacaacg gcgcatcctg acggaactct ggcgtacggc
    73981 ccatgactgc cgccagatca aagcggaaac catcgacgtg gcaggtttct acccaataac
    74041 gcaggcaggc gctggcataa tccaccaccg ccggatgact caaattgagc gtgttgccgc
    74101 aaccggtcca gttgtgataa tcgccgtctt ctcttatcca ataatagcta cggttatcga
    74161 tcccacgcag cgagaataac gggccgtcga ggtccagttc cgcactatgg ttgagcacga
    74221 tatcaagaat gacttcgata cccgctttat gcagtgcttt gattgcatcg cgaaactcat
    74281 ccagcgccgt ttctggcgag caggcatacg ccggatgcag cgcaaacatc gccaccgggt
    74341 tgtaacccca gtagttactt agccccatgc gttgcagacg tggttcactg gcaaactgcg
    74401 ccactggcag cagttccagc gcggtaatgc ccaattgttt caaatagttg atcatcaccg
    74461 gatgcccgag ggctttataa gtgccacgga tctcgaccgg gatctccggg tgcaagtacg
    74521 ttaatccttt gacatgggct tcataaatga tggtgctgcc ccacggcgtg cgcggcgggg
    74581 catcatcttc ccagtcatag tgatcaacca ccactacgca tttcggcgca atggcggcgt
    74641 tgtcgcgata gtcaggttca ttatgaccgg cgtgcagcag cgggttatct ttaaactccc
    74701 cctcaatttg ccgcgcgcaa ggatcaatca acaacttcgc cgggttaaag cgatgcccct
    74761 cggcgggttg ccaggggcca tgaacgcgat aaccataacg caaacccggg cgcgcatccg
    74821 gcagataacc gtgccaaatg tcgccactgt gccctggcaa gtcatagcga tgttcctggc
    74881 cattggcgtc aaagacacac agttctaccc gctcggcatg agcggagaaa agtgtgaagt
    74941 tgacgccctg accgtcgtaa tgcgcgccga ggggagcggg tttgccaatg gcgagttgtg
    75001 tcattctgcc tcccgaacca gccagatagt ggccagcggt ggtagcgtca ggcttagtga
    75061 atgctgacga ccgtggctgg caatctcatc gctgtgtacc gtgccgccat tgcctgcatt
    75121 actgccgtga tagtgcatgg aatcggtatt gaggatttca cgccatttgc ccggctggtt
    75181 tatgccgaag cgataatcat gacgcggcac cggcgtaaag ttactggcaa cgatgatttc
    75241 gttaccctct ttatcgcgac gcacaaagat cagcaccgag cgttctttgt catccaccac
    75301 cagccattca aagccgtacg ggtcaaaatc cagttcatgc attgctttat ggtggcggta
    75361 ggtgaggttc agatcgcgca ccagacgctg gacaccgtgg tgccagttat cgccgccttc
    75421 caacagatgc cagtcgaggc tggcgtcatg gttccactcg cggccctggg caaattcgtt
    75481 acccatgaac agtagtttct tgcccgggaa tgcccacatc cagccatagt aggcgcgcag
    75541 gttcgcgaat ttctgccatg cgtcgcccgg catgcggtcg agaatcgatt ttttaccgtg
    75601 gaccacttca tcatgcgaca acggcaggac gaagttttca gtgtagttgt agagaatccc
    75661 gaaggtcagt ttatcgtgat gatactgacg ataaaccggg tcgagcttca tgtagtccag
    75721 ggtgtcatgc atccagccga ggttccactt gtaccagaag cccagaccgc ccatatcctg
    75781 cggacgagaa acgccaggga aatcggtaga ctcctcagcc attgtcaccg caccggaaac
    75841 ctgctcacca agaatacggt tggtattacg caagaattca atcgcttcaa gattctcgcg
    75901 cccgccaaat tcgttcggga tccactcccc ctctttacgg ctgtagtcgc gataaatcat
    75961 tgacgccacc gcatcgacgc gcagcgcatc aataccaaaa cgttcaatcc agtaaagcgc
    76021 gttaccgacg aggaagttac tgacttcacg gcgaccatag ttgtagatca gcgtgttcca
    76081 gtcctgatga tagccttcac gcggatcgct gtgttcatac aagttcgtgc catcaaattc
    76141 ggcaagcgca aagtcatcag tcgggaagtg gcctggcacc cagtcgagaa tcacgttcag
    76201 accagctgcg tgtgcggcat caatgaaata acggaagtcg tcgcgagtac caaaacggcg
    76261 ggttggtgca tacaggccgg ttggctgata accccaactg ccatcgaagg gatgctcgtt
    76321 aatgggcagt agttcgaggt gggtaaagcc catccattta gcataaggca ccagttgatc
    76381 ggccagctcg cggtagctca accagaaatt gttgtcggtg tggcgacgcc aggaacccag
    76441 gtgaacttca taaatagaga ttggcgcatc aaactgattc gcttttttgc gctcttcagt
    76501 ctgtacaacc ttttccggca gcccgcaaat aagagacgcg gtttccgggc gcatttgcgc
    76561 ttcgaaggca taagggtcgg acttcagacg caagttgcca ttggcatcaa tcatctcgta
    76621 tttatagagc tgaccgttat gcgccccagg gataaacagt tcccagatgc cgctctcttt
    76681 acgcaggcgc atcgggtgac ggcgaccgtc ccagtagttg aattgcccaa ccaccgagac
    76741 ccgacgggcg tttggagccc agacagagaa acgcgtacct gtgacgccat ccatagtatc
    76801 tgcatgcgcg cctaaggttt catacgggcg caggtgagta ccttcagata atagccaggc
    76861 atccatttcc tggattagcg gaccaaaacg gtaaggatca tcaatcaggt tttgctgacc
    76921 atgccagaca acagccaact gatagcggaa aaaattctta cgtcgcggaa tgacgccgct
    76981 aaagaatccc cgtgagtcga gacactccag ttttgcgagt ttgcgcccgg ttttcggttc
    77041 aatcacccac acatcggtag cgtcgggtaa aagggcacgg acttccagtc ccgcggtggt
    77101 tttatgcatt cccagtacgg aaaaaggatc cgcaaaatgg cctgcaatta gcgcgttaat
    77161 cacgtctcta tcgatacgat cggacatgct tgtcttcctg ttttattgtg tcaccccatc
    77221 caattggatt tttgacttct ggttgtgaca tttttttgac ctgaacggcg cagcactctg
    77281 tgcatcctct ctgcgtcgtc ctcacttcag gtaaggctgt gaatactcat gtattcagcc
    77341 acccttaaag aatagccaat gctctattta actcccggta aatcatgaaa catctgcgct
    77401 tactcctgta ttacgcacta acaggggcgg catcgcgccc cagatttaat gaataaagat
    77461 tacgccagtt gacgaagcat ccgacgcagc ggctccgcgg ccccccacag cagctggtcg
    77521 cccacggtaa aggctgacag gaactctggt cccatattca gcttacgcag gcggcctacc
    77581 ggcgtggtca gcgtgccggt aacggcagct ggggttagct cacgcatagt gatttcccga
    77641 tcgttcggaa cgactttcgc ccacggattg tgcgcagcca gcagttcttc cacggtcgga
    77701 atagacacat cttttttcaa tttaatagtg aatgcctggc tgtggcagcg caatgccccg
    77761 acacgcacac ataaaccatc taccggaatt acggaagatg tgttgaggat cttgttggtt
    77821 tccgcctgcc ctttccactc ttcgcggctc tgaccgttat cgagctgttt gtcgatccac
    77881 ggaatcaggc tacccgccag cggcacgcca aagttatcca ccggcagctc accgctacgg
    77941 gttaaggttg tgactttgcg ttcgatatcg agaatagcag aggacggggt cgcgagttca
    78001 tctgccacat ggccatacag atggcccatc tgggttaata actcacgcat atgtcgcgca
    78061 ccaccgccgg aagcggcctg gtaggttgca acggacaccc aatcaacaag atcattggcg
    78121 aataaaccac ccaacgacat caacatcagg cttacggtac agttaccgcc aacaaaagtc
    78181 ctgatgccat tatttaatcc gtcggtaatg acgtcctgat tgacggggtc aagaatgatg
    78241 atggcgtcat ctttcatgcg cagagacgat gctgcgtcaa tccagtaacc ttgccatccg
    78301 ctttcacgaa gctttggata gatttcgttg gtataatcgc cgccctgaca ggtcacaatg
    78361 atatcgaggg cctttagcgc ctccagatca aaggcatcct gaagtgtgcc agtggttccg
    78421 ccaaaagacg gcgcagcctg gccaagctga gaagtagaaa agaagacagg gcgaatggcg
    78481 tcgaagtcgc gctcttcaac catgcgttgc atgagaacgg agccgaccat accgcgccag
    78541 ccgataaaac caacattttt cataagcgtt tttttcctgc aaagatgtgt gctgtataaa
    78601 tgtgccggtc tcctcttggc acatctttca ccatacaaaa agcagccaaa gtcgcaagtg
    78661 aaattaatca atgatagcga agccatcagt aatgcgactt atcctgcttt gttagcacgc
    78721 agaaagtccg cggcaattat cagggaattt gagttatgaa tgaaatcatt tctgcagcag
    78781 ttttattgat cctgattatg gatccgctcg gaaacctacc tattttcatg tccgtactga
    78841 aacatactga accgaaaaga cggcgggcaa tcatggtgcg agagttgctt attgctctcc
    78901 tggtgatgct ggtgttcctg tttgcgggtg agaaaattct ggcatttctc agcctgcgag
    78961 cagaaaccgt ctccatttct ggcggcatca ttctgtttct aattgccatt aagatgattt
    79021 tccccagcgc ttcaggaaat agcagcgggc ttccggcagg tgaagagcca tttatcgtgc
    79081 cgttggcaat tccgttagtc gccgggccga ctattctcgc cacgctgatg ttgttgtctc
    79141 atcagtaccc gaatcagatg gggcatctgg tgattgctct gctgctggcc tggggcggca
    79201 cctttgtcat cctgctacag tcttcgctat ttttacgtct gctgggcgag aaaggggtga
    79261 acgcacttga acgcctgatg ggattgattc tggtgatgat ggcaacccag atgttcctcg
    79321 acggcattcg aatgtggatg aaggggtaac gaggagagtt tttcgaaaag aacccggtag
    79381 cgtcacaact accgggcgaa caaacttaac tcaacagctg gaacgcaatc atcccaacga
    79441 tggcaccgac agtgccgagg atggtttcca tcatggtcca ggttttcagc gtttcggctt
    79501 cggtcgcgcc ggtaaattta ccgaacaacc agaaaccggc gtcgttaacg tggctgacaa
    79561 caatcgaacc accagcgata caaatcgaca gcgccgccat ttgcgcaccg gagtagttca
    79621 gttgttcaat aaccggcatc accagtccta ccgccgttaa acaggctacg gtggcagaac
    79681 cctgaatgat gcgcactgca gctgccagca cgaagcaggt gatagcaatc ggcaggccca
    79741 tgccggttaa cgcttcgccc agtgccggac ctacgccaga gtcaaccagc acctgtttga
    79801 acacgccgcc tgcaccaatc accagcagaa tgatccccgc cggttgcagc gcgtgaccgc
    79861 aaatctccat cactttgtct tttggcatgc cctgacgcat tgccaggcca taaatcgcta
    79921 ccagacaagc aaccagaatc gcggtaaacg gatgaccaat aaactcgaac cattcgtaag
    79981 cggtagagcc ttctggcaca aaacgcgcgg caatggtttt cagccccacc agcaccagcg
    80041 gcaacaggat cagcgacagg ctgaatccga aagatggcat tttgccttcg ccgagatgcg
    80101 gttcgctgat gtcgtcagga atatgcaact ccacgtagcg gctgatgaaa ttaccccaca
    80161 gcggcccggc aataatcatt cccggaattg ccgcacacag gccaatcagg atcatccagc
    80221 caaaatcggc gttcatctgc gatgccagca gcattggcgc tggccctggc accaggaacg
    80281 ccgccgctgc cgccacgcct gcgaataatg ggattaccag cttcaccagg ttcgtaccgg
    80341 tgtggcgcgc cattgagaaa gcaacgctaa tcagcagaac tatcgccact tcaaagaaca
    80401 gcggtagcgc acagaccaac cccgcaaggc cgatggcaaa atgcgcgcgg ctgtgaccga
    80461 aggatttgag cattttgacg gcaatctgat cgactgcgcc ggtttcatgt aagatcttgc
    80521 caaacatagc tcccagggcg acaaccaccg ccaggaagcc gagggtgcct cccatccctt
    80581 tttccatcgt cgctgcgatt ttatcgagcg gcataccgga aaaaaggcca gcccccatgg
    80641 acaccaccat caaagccagg aaagcgtgca tacgcgcctt catgactaaa aacagcagca
    80701 gtaaaacaga ccctactgct gttaaaacaa gcgttaatgt agtcactact tatttgcctt
    80761 ttttaataac ctcaatggtg cttgccacaa caccttccag cggttgatcg atatccacca
    80821 ccagtacatc ggtttcgtcc gcacccggct cctgcagcgt ttcaaactgc gtcaccaaca
    80881 tttgggtttt aaagaaatgg cctttgcgcg ctttcaagcg actttcaatc acatcaaaat
    80941 cgcctttcag gtagatgaaa gagagattcg gattaccttc acgcagcaag tcgcgatagt
    81001 gctttttcag tgcagaacag acgatcagcg acactttatt ggtgcgctgc atagcaaacg
    81061 cggcgtcgtt cagcgcctgc agccacggtt tgcgatcgtc atcattcagc ggttcgccag
    81121 acgccatttt ttcgatattg cgccgtggat ggaggaaatc gccatcaaga aacgcggcat
    81181 gaagttgatg cgccacttca ctggcgaccg cagatttgcc gctgcccgat acgcccatca
    81241 agacgtaaat gtggtgatca tggttagtcg tgctcaaagt ggtgccccca caatacaaga
    81301 attaaaaatg ttacgggtaa ctgttatcgg taacattgtc cagccggaca atatcagaag
    81361 caatatccat ccgtgcctta agtgtataag tgtgagctac ttcaaatttg tgggcttaaa
    81421 tagatccgcc cggtgacaag gtgaaaccta aatctaacat tttcggtgtc acagattcgc
    81481 cacgaatacg cgccagcagg cgttcagcgc caatactgcc catccgctca cgcggcgtca
    81541 gcacgctcgc cagtcgtggc tccatcacct gaccaatgtc atgaccgtgg aaaccggcaa
    81601 tcgccatatc gtcaggaact tttaacccca gacgctgaca ttcaaacgcc gcgccgaccg
    81661 ccagatcatc attagtacag aacacgccat ccagctgcgg atattcccgc cgcgcctggc
    81721 gaatcagttc aataccggaa gagtaagaag aagattgctc aaccatcacg ctatacggca
    81781 ccaggcctgc atccagcatc gcctgttcgt atcccttctg tttgatgata gtacgttcgt
    81841 cgagacgtgc gccgagatag gcaatgtggc gatgcccgcg cgcaataatg gcggtagtca
    81901 tctggcgtgc cgcttcaaag ttatcaaaac cgacggcgat atcgaggcac ggcgactggc
    81961 tgtccatcaa ctccaccacc gggatccccg ccacttcaat catctttaag gtgcgcggcg
    82021 tgtgggtacg ttcggtgagg atcaggccgt cgatattcca ggacagcatg gattcgagac
    82081 gttcttgctc catttccggt ttataaccgt agtgcgccag catcgtctga taaccgtgcg
    82141 cgtcggtgac gctttcgatt ccgcgtaata cttccgcgaa aacctggttg gtgagagaag
    82201 gtaacaggac gccaatcgcc cggctggtag cgttagagag gatatcgggc gcacgattgg
    82261 gaatatagcc cagttcatca agagcggcgg caatcttgcc gcgtagagcg acggaaacct
    82321 gctccgggtt gcgtaaaaaa cggctgaccg tcattttggt cacgcctaca cggtcagcca
    82381 catcctgaag tacgggtctt ttctttttca tcgtcctgaa ggtacaaaag agatagattc
    82441 tcttagttta acacggacgc atcagaacct tcccggggaa aacagtgctt gtttacaatt
    82501 attagatata cactaaatca attgcatcgc attgtgctaa cgacgcggca aaaccccgga
    82561 agcgtgcatc agcacgcgac cggagcgagc gaggaagcct gcaaaaaggc aggcttcaaa
    82621 gatgccgttg agttttaaac cggcggcaga tcgaacagta acacttcgct gtcgctatcc
    82681 gcatggatgg agattgcctg ctcatcccag attgccagac catcgctggt cgaggctttc
    82741 acgccgttaa tggtgacatt gcctttcacc acctggatcc agacgcggcg ttcagcggca
    82801 atctgatgca ccgactgctc atctttcagc aacgcccaac gatacagttc catatcctga
    82861 tgcactttca gcgaaccatc tcgcgcatcc ggcgagagca ccagctgttt gccctgcacg
    82921 gcatcgaagc gacgctgttc ataacgcggc gtaatgccat tttcttcagg catgatccag
    82981 atctgataca gatgcagacg ctcggtgctg tttgggttgt actctgagtg acgaataccc
    83041 gtacccgcac tcataatctg gaactcaccc gccggaacct gctctttatt gcccatgctg
    83101 tcctgatgct caacagtacc ttccagcacg taggtcaaaa tttccatatc tttatgcggg
    83161 tgagtgccga agccctgccc tgcttcaatc acgtcgtcgt taatcacgcg cagcgcggag
    83221 aagcccataa agttcggatc gtaatagttg gcaaaagaga aagtatgcca ggagtccagc
    83281 cagccatgat ttgcatgacc acgttcattt gctttgcgga agtagatcat ttgtattcgc
    83341 cccctgaatg atttcgatgg gcttagtgtg gacccgatct gcctgggatg atagagggtg
    83401 aaaattgacc cctctgttca aaaaaattga actattcgag gggccatcaa acttacttag
    83461 cgagagttac tgtggaggga gaggcttgct caaatccgcg ttcaaggatt tccagattgg
    83521 taagaacttc agattccttg acgtaatttg gcgcaccgtg ggtgatggtt tgatacaacg
    83581 catcataaac gcgcccgtaa tcgcccatct ccggcttcat ctcttctctg accgtcacgc
    83641 cctcgtcatt gacatactcc agcacaccga ccgaatcatc cgctgcgaat cccggttcgc
    83701 ccggcataat attagccttc aggctggttt cctgctggtc gataccgtat ttaataaacg
    83761 aacctttctt accgtgaacg ataaatttcg gataatcgat tttcaccaga tggctggttt
    83821 tgacgatggc tttcaggtcg ccataaaaca gttgcgcttc aaaggtgtcg tcaggattgg
    83881 ctttattacg caggctgcgg atgtcataag cgacgtgatc cgggcgaccg aacagagaaa
    83941 taatctggtc catcgtatgc acaccaaggc cataaaacgc gccatcctgc ggcagcccag
    84001 gtttggtttc tgccaccggg cggtaatagt caaaatggct ttccacttca acaatctctc
    84061 ccaacttgcc actttcaatc gcttttttcg ctgtcaggaa gcaggagtca aagcgacgat
    84121 tctgatacgg cgtgacggtc agccctttgc ttttcgccaa cgcaaacagc tccttcgcct
    84181 gggcaagcgt cggggtgaac ggtttttcga ccagcacatt tttcccggct tccagcgcgc
    84241 gtttcgcgta ctcgaaatgg ctgtccgcgt gggtgcagac aacaaccagc ttaacatcgg
    84301 gatcgtttag tacttcgtcg agatcgctgg tcaaatggat atgggaataa atgggagcct
    84361 gttcttccgg cttcgcatgg cgacgaaaaa tatgcgcgac atgccagcta tccttgcggt
    84421 taagtacata cggcagatgg taacgggtgg tgcttttgcc gaagccaata aaggcgcagt
    84481 tgatgaccat gatttcgtcc tttttaaggt ggttatttac accttagcgc aaagcggacg
    84541 tggttcctac tggagtgcgc ataacgaaca caagcactcc cgtggataaa ttgagaacga
    84601 aagatcaaaa aaaaagccag cacccggctg gctaagtaat actggaagca atgtgagcaa
    84661 tgtcgtgctt tcaggttctc cgcgagggtc ttcctgatcg cgagacaata ataatcattc
    84721 tcattcgcac ttgtccaaca ctttttgcaa aaaaatgcat ttgactcgca tttgaaagtc
    84781 aatgatgttg aaagggacat ttaccccaaa gaggacaaag gaatgagtga gatagtaata
    84841 cgccacgcag aaacacggga ttacgaggcc atcaggcaga ttcacgccca gccggaggtg
    84901 tattgcaaca cactacaggt gcctcatcct tccgatcata tgtggcagga gcgactcgcc
    84961 gatcgtcccg gcatcaagca actcgtcgcc tgtattgatg gagacgtcgt gggccatctc
    85021 accattgacg tgcaacagcg cccacgccgc agtcatgttg ccgattttgg tatctgtgtc
    85081 gactctcgtt ggaagaaccg cggcgtcgcc agcgccctga tgcgagagat gattgaaatg
    85141 tgcgacaact ggttgcgggt agatcgtatc gagctaacgg tgttcgtcga taacgcgcca
    85201 gcaattaagg tctataaaaa attcggcttt gagattgaag ggactggcaa gaagtacgca
    85261 ttacgtaatg gtgaatatgt cgatgcgtat tatatggcgc ggatgaaata gagaatttgc
    85321 aatctaagtt tgtcatttaa gccctcacct accgggagag ggcttaagat attagtaacc
    85381 cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac cgtccggccc
    85441 aaccataatg ctttgtgtac tgcccatcgc ctctttcagc gccactttct gaccttttgc
    85501 ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct cgtccggcaa
    85561 ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc cataatcgat
    85621 gctattcacc accatttgca gcactgtagt gatgatccgg ctaccgcctg ggctaccggt
    85681 aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg acagcgggcg
    85741 tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt tcggtacgcc
    85801 cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct cgcccgcgac
    85861 aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt taccatcttt
    85921 atccaccact gagtaatggg tagtttgatt actctcataa ggcgcaagct tgccggggcg
    85981 aatttcgctg gatggcttcg ctttattgat atcaatttga tcggcaatag atttggcata
    86041 ggctttattg gtcagcgcct gccacggtac tttgacaaaa tccgggtcgc caagatattc
    86101 cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg catcggcgct
    86161 gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt gtacgatatg
    86221 gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat aatcgccgct
    86281 tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga tcaagccacc
    86341 gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga attcgtccgg
    86401 gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca gcgtgtcgcc
    86461 ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt gattcggcaa
    86521 cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga taaaaccatc
    86581 gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc cgtatttatc
    86641 cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat gcgaagtgag
    86701 tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg tcgctttggc
    86761 gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc ggattaacat
    86821 aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg cgtagcccac
    86881 cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc ccacctgagt
    86941 ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccgggt ggaagacatc
    87001 ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc taaaacaact
    87061 tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta tcatcgttat
    87121 tctccagaga ttaaggggca accccagcta agcctggtat ataactctga attaatcatc
    87181 gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac gcgatgaaac
    87241 gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc attaatactc
    87301 tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg caaacccaga
    87361 tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa acgcaaactc
    87421 agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg gtgttgcagt
    87481 cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc aacggcggga
    87541 tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg ctgccggaga
    87601 ggagaaacgg cgacatgctg aatcagccca gcacgccgca gcctgacatt ccgttgaaaa
    87661 ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga tgcaatccac
    87721 gccccagcgc agcaactctg ccgcgcgctg gggtttattg acggtataaa ccagaatccg
    87781 cagtccggcg tctttcaact gcatcactcg cactttatcg agtaacttat gattgagatg
    87841 aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc actcatcaag
    87901 caataaacca cgcggcagtt ccggtgccgc cagctgtgca gcttctaaag catcaatctc
    87961 aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg ccagcgccac
    88021 cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat tcgccatcat
    88081 cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct cacctttaaa
    88141 cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt tcagttcacc
    88201 cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca ggaagatctc
    88261 gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt ttgccccgac
    88321 gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat gagcgacgat
    88381 gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa aagatgcagc
    88441 tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc ctgatgcgcc
    88501 agtcgcacca ccagcttctg ctcgccccag cgtccgtgcg ccaggttatc tgcgccgagg
    88561 atctccagcg tgtccatcac cagcggtacg ccgccttctg cctgcgagct tagcgcaata
    88621 tgttccgggc gaatgccgag agtcatttta cgcccggcat actgacggta gccaccgttt
    88681 agcggcagct caataccgcc gtccagttcg aaatgcgtgc cttcgttatt cacgcggcct
    88741 gtcagcaggt tcatcgccgg gctgccgata aaactcgcta caaacaggct ggcgggcttt
    88801 tcgtagactt caactggtgt gccaatctgt tcggcaacgc cgccgttcat caccattact
    88861 cgctgggcga gcgtcatcgc ttcaacctga tcgtgagtaa cgtagagtga agtcgttttc
    88921 aggcgacggt gcaactgttg cagttcaaga cgcatctgca cgcgcagctt ggcatcgagg
    88981 ttagagagcg gctcatcaaa caggaacacc gccggatcgc gcacaatcgc gcggcccatc
    89041 gccacacgct ggcgctgacc gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc
    89101 agctccagaa tgcgcgccgc ttctttaacg cgctcggcaa tttgctgctt gcccatgccg
    89161 cgaattttca gcccccacgc catgttttct tcgacactca tatgcggata aagcgcgtag
    89221 ttctggaaca ccatcgcaat cccgcgatct tttggctcca tttcagtcac gcgctggtcg
    89281 ttgatccaga tatcgccttc tgtcacccgc tccagcccgg caaccatgcg cagcagcgtc
    89341 gatttcccgc agccagacgg cccgaccatc acgataaatt cgccatccgc cacatcaagg
    89401 gtcagcggtt taatgacctg ggttttacca tcccagcttt tggttactgc ctgtaatttc
    89461 agtcctgcca tcttatttct cactatcgac caggccgcgc acgaaggcac gctgcatcac
    89521 taaaacaatc accaccggag ggataagcgt taacaacatc gccgccatca ctgagttcca
    89581 ttcagtggtg ccttcgcctg tagcgatcat ccctttgatc cctgccacgg tggtgccgag
    89641 atccacatcg gtaataatca acaacggcca caaatactgg ttccagccgt aaataaaggt
    89701 gatcacaaac agcgccgcca gattggtttt ggagagcgga aaaacgatgt cgcaaaagaa
    89761 acgcattggt gacgcgccgt cgatccgcgc cgcttccacc agttcatccg gcagcgtcat
    89821 aaagaactgg cgaaacagaa acgtggcggt cgccgaggcc atcagcggca gcgttaaacc
    89881 ggcgtagctg tcgagcatct tcaggttggc gatgacttcc accgtcggga agatacgtac
    89941 ttcaaccggc agcatcaggg tgataaaaat catccagaag aagaggttac gtagcggaaa
    90001 acgaaaccag acaatggcaa atgccgagag catcgagacg gtaattttgc cgagcgtaat
    90061 gctgaacgcc atcacaaagc tgttaagcaa catccgccag aacggcgcgc tattcgtgcc
    90121 taccccgttc acccagatgt tgtggatgtt ttccagcaga tgtgtgccgg ggatgagcgt
    90181 catcggcgcg gcatagacgg cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag
    90241 cgggaagagg atcaccgcga tcccgaggat cagcatggta tggctgaata tcgtcagcca
    90301 cggacggttc tcaatcattg gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc
    90361 gtcagcacga tgacgaggaa catcaacacc accgactgtg cggcagacga agccagatcc
    90421 agtccggtaa aaccttcgcg gtagatctta taaatcagcg tcgtggtggc ctgaaccggc
    90481 ccgccggacg tggcggcgtc gatcaccggg aaggtgtcga agaaggcata caccaggttc
    90541 actaccagca ggaagaaact caccggggcg ataagcggca gcgcaatctt aaagaagcgg
    90601 cgaatcggcc ctgcaccgtc gatggctgcg gcttcgatca acgaacgggg aatggattgc
    90661 agcgcggcat agaagaacag gaagttgtag ctgatttgct tccatactga ggcaaacacc
    90721 accagaaaca ttgcctgacc gctgttttgc gcgtggttcc agtcgtagcc gaactccgcg
    90781 agaaaatggg tgatcagccc gcgaccgggg ttaaacagga agatccacaa tacggcggca
    90841 acggcgggag ccacggcgta aggcagcaac attaaggttt gatagaaacg gctgccgcgc
    90901 acgatgtact ccaccagcgc cgcgaagaac agcgacacca gcaaaccgct gacggtgaca
    90961 aaggtgctga attttatcgt cgtccagaag gagtcgagat agtagctgtc atgaaacagc
    91021 gcgacgaagt tatccaggcc gacaaactgg ctggagaaac caaacggatc gacgctttgt
    91081 agcgagtacc acaacgcttc gcccgcaggc cagataaaaa agataacggt gatgatgagc
    91141 tgcggcgcga ccagcagata aggcagccag cgcgagcgga acaccggacg ggatgatgac
    91201 attacggtta attcctgaac tgtgccggat gcgcttcgct tatccggcct acacgcgatg
    91261 ccaggtcaga taagcgaaac gcatccgaca ttacactgat taagacttcg tcgatttctc
    91321 aaagcggcgc agcaactgat ttccacgctc aacggcggta tccagtgcct gctgtggtgt
    91381 cttcttaccg gtccacacgc tctccagctc ttcatccaca atcacgcgga tctgcggcat
    91441 gttgcccaga cgcagccctt tggtgaacgg caacggcggc ttgttcagca tctgacgcgt
    91501 cgcggtatcc gcccctgggt ttttctcata aaagccctgc tcacgggtca ggtcatacgc
    91561 tgctttggtg attggcagat aaccggtttt ctgatgccac tcggcagcgt tttctggctt
    91621 cgcgaggaag tcgaggaact tcgccacgcc ggtatacgtt tctttatttt tgccctgcat
    91681 cacccacagg ctggccccgc cgataatggc gttttgcggt gcatctttcg catcggcgtc
    91741 gtaaggcatc atgcctacgc catagttaaa tttggcgtac tcacgaatat tagcgagaga
    91801 accggaagag gcggtggtca tcgcgcaatc accgttatag aacttctcgg tggattcatc
    91861 cttacgaccg acgtagctga agtcgccctt cttgttcatc tcctcgagca tggcgatgtg
    91921 tttcacctgc tccggcttgt tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt
    91981 tttgctggca aacggcagac cgttccaggc gctaaagttt tccagttgga tccagccctg
    92041 ccagccgctg gcgtagccgc acttcatacc ggaggctttc agtttcgcgg catagtccgc
    92101 cagatcctgc caggttttcg gcggttgttc cgggtctaat cctgctttct tgaaggcgtc
    92161 tttgttgtaa tagagaacgg ggtcgagctg ttgaatggct gggagagtaa gtggcccgtt
    92221 ttgctgtcgg agtagtaacc ggaaaccgtc ggcacaaact gcgactcatc gaattgaatc
    92281 cccgcctctt taaacacgtc atataccggt ttaatggctt tcgatgccat catggtggcg
    92341 gtgccaactt cataaacctg caaaatagcc ggagcgttgc cggtacgaaa tgcggcaatc
    92401 cccgcgctta aattctgttc gtagttgcct ttataggtcg gtacaatttt gtaatccgga
    92461 ttttcggcgt taaaacgttg ggccagagaa tccacctctt tacccagttc cccttccata
    92521 gaatgccaga acggaatggt cgtcactgcc tgtgcattcc ccattaacgc cagtccgagc
    92581 gccagtgctg aagctgtata atgtaacggt ttcatcgttt atctctcttg ttgtaccgaa
    92641 tgcgcgaatt cacgcgtttt atgctcgcgg ggtaacatga catgctcgaa ttacagaaaa
    92701 ataacttttt tgttacattt gtaagatagt aaggtgtcag aaagatgaca aggcggtgac
    92761 ggcgtgggtg agggaaaatg ggagatgggg cacggataag cggaaaatag aaggtctgaa
    92821 tcaaactcta cagattactc atcgtttcat gccggatgcg gcgtaaacgc cttatccggc
    92881 ctacaagatc gtgcaaattc aatatattgc aactcaccca gtaggcctga taagcgcagc
    92941 gcatcaggca attttacatt tgtcacctgt ctcaaaggag tcttttgact ccttatcaat
    93001 caacgtgtta ttacccgcct aaatacgcac ttctcactgc ttcgttcgcc agcagcgcgt
    93061 cgccggtatc ggagagcacc acatggccgt tttccagcac gtagccgcga tccgccagct
    93121 ttagcgcctg gttggcgttc tgctcgacga ggaagatagt catcccctgc tcgcgcagct
    93181 gctcgatggt gtcgaaaatt tgctggatga taatcggcgc aagaccgagc gatggctcat
    93241 caagtagcag caaacgcggg ttgctcatca gcgcgcgacc aatcgccagc atttgttgct
    93301 caccgccgga catggtgcct gcccgctgaa cacggcgctc atgcagacgt ggaaacagtt
    93361 catacaccca ctttatgcgc tcctggaact ggtcgcgttc ggcaaaaaag ccgcccatcg
    93421 ccaggttctc ttccaccgtc atccgcgaga agacgcgacg cccttccggg acaatcgcta
    93481 ccgcttcgcg catgattttc gccgtctgcc agtcggtaat gtctttatca tcaaacacaa
    93541 ttcgcccgct ggtagcgcgc ggatcgccgc ataacgtgcc gagcaatgtg gttttccccg
    93601 ccccgttcgc gccaatgagg gtgacaatct cgccctgatt gatatgcagg ctcacctcat
    93661 gcagcgcctg gattttgccg tagtgggcgc tgactttgtc aaaggacaac atgacttttt
    93721 ccatcttatg cctcacctaa ataggcacgg atcacgtccg ggttattacg gatttgctcc
    93781 ggcgtaccgt ttgccagtgg cgtcccctga ttgaccacat aaattcggtc tgaaatcccc
    93841 atcaccagct tcatatcgtg ttcaatcaac aagatagtgg tgttgtgatg attgcgcagt
    93901 tcggcaatca gctcatccag ctctttggtt tcttttgggt taagacctgc cgccggttcg
    93961 tcgagcatta aaatctccgg ctgcgtcacc atgcagcggg caatctccag acggcgttgg
    94021 tcaccatagg ccaggttact cgcctgacgg ttggcgtgtt ccagcaaacc aatgcgctca
    94081 agccaggtgg cggcgcggtc gagcgcttcg ctctgggcgc gacggaaaga aggcgttttc
    94141 aacaggccag agaacagccc ggttttcagt tgttgatgct gcgccaccag caggttttca
    94201 atcaccgtca tttcacggaa cagacgcaca tgctggaagg tacgcaccac gcccatgcgg
    94261 gcaatttgct gccccggtaa accttccaga tgctgatcac gcagtaaaat agtgccgccg
    94321 gtgggtttgt agaatccggt caggcagtta aaaaccgtgg tttttccggc accgttaggg
    94381 ccgattaacg agacgatctc ctgcgggtac agttcaagat tgacgttgtt caccgccagc
    94441 aagccgccga agcgcatcat cagaccgtta acagataata atggctgact catgcctgct
    94501 ctcctttcgc tgcgccgttt ttcagcttca gttgcgggcg cgtcatgggc agcaagccct
    94561 gcggacgcca gatcatcatc agcaccatca aaccaccgag cattaacatg ctgtattcgt
    94621 tgaaatcacg catcaattcg cgcgatacca ccagcaaaat tgccgccaga atcaccgcaa
    94681 actgcgatcc cataccgccg agcaccacta tcgccagtac aaacgccgat tcagcaaagg
    94741 tgaaggattc cgggctgaca aagccctggc gcgcagcaaa cagcgttccg gcaaaaccgg
    94801 caaacgcggc acttatggta aaggcagtca gcttgatacg acgcgggctt aagcccagcg
    94861 aacggcaggc gatctcgtct tcacgcaacg cttcccacgc acgccccagc ggcatccgca
    94921 gcaggcggtt aatgacaaac aggcttagca ccaccagcag caacgccacc aggtagagga
    94981 agatgacacg atcggaggga tcgtatttca ggccaaagaa attactgaac gtgtcccagc
    95041 cgccttcacg agcggtacgg ctgaactcga gtccgaagag tgtcggtttc gggatctgac
    95101 tgattccgtt cgggccgccg gtaatttcgg tgttattgag cagcaatatg cgcacaattt
    95161 cgccgaaacc gagggtaacg atcgccagat agtcgccgcg caagcgaagc accgggaagc
    95221 cgagcaggaa gccggccgcc gctgccatta acccggcaat cggcagacag gtccagaagc
    95281 ccaggccgta atagtgattg agcagcgcaa aagtgtaagc gccgatggcg taaaagccgc
    95341 cgtaccccag caccagcaga ccagaaagac caacaaccac gttcagcccg aggccgagga
    95401 taatgtagat catggttagg gtggcgatat ccaccgtccc acgcgacacc ataaacggcc
    95461 acgccaccgc aagcaccagc agcgccacga ggaatagttt ctgcttcacc gtggagccat
    95521 caatcgcggg cagaataaac ttcggtccgg aaacgctttt tagccctttc tggaaagccg
    95581 gtcgcaaaag ctggaagaaa aagaccaccg ccgtgccgat aaacacccac tgccaacgga
    95641 tgtctgaagc cgtgtcgacc accagtttgg tgccatccag ctccagttgc acgcccataa
    95701 agacgcccgc caggacaaaa aacatcgcgg cagagagcag cgccattgca atatgcatcg
    95761 gtttcatact ttctctacct ccgggcgacc cagaataccg gtcggcatca ccagcagcac
    95821 cagaatcagc agggcgaatg agaccacatc tttatattcc gtactcagat aggcagaaga
    95881 gagcgcctcc gcaatcccca gaatcagccc gccaatcatc gctcccggaa tgctgccaat
    95941 cccaccgagc accgcagcgg taaaggcttt catcccggcc ataaagccga tgtaggggtt
    96001 aattacgccg tagaactgac cgagcagcac acctgccacc gccgccatcg ccgcgccaat
    96061 cacaaaggtc agcgcaatca cccggtcggt gttaatgcca agcaggctcg ccattttcag
    96121 gtcttccgcg caggcacgac acgcgcgccc catacgggaa tagcgaatga aaaccgtcag
    96181 cgccagcatg gcgaggaagg tgacaatcca gatcaccgcc tgcatggtgg taatagaggc
    96241 agagaagttt tcgctatgcc ccaccaccca ctgaccgtta aacagactgg gcagcgccac
    96301 gtcacgcgaa ccttcagtca ggctaacgta gttttgcagg aagatggaca tgccgattgc
    96361 cgagatgagc gcaatcaggc gcttagagtt acgcaccggg cgataggcta cccgttcgat
    96421 actccagccg taggcgctgg cgatgacgat tgcgccgacg aatcccgccg ccaccagcag
    96481 ccagccggta tcaatgccca tcatcatcag cgcggcgatg atcataaatg agacatagct
    96541 gccgatcata taaacctcgc cgtgggcgaa gttgatcatg ccgataatgc cgtaaaccat
    96601 ggtgtagccg atggcaatca gcgcgtaggt actgcccagc gtgacgccgt taaacatctg
    96661 ctgcaagaaa tataaaaact gctcagacat aaggtaacct ttctaaaccc gcccgttttt
    96721 cacgggcggt gggatgatca cttggctgcc gtggatgaac cgtcggcgtg ccactggaag
    96781 acaccaaaat caaatccctt aagatcgcct ttttcatccc agttcagcgg cccaatcacg
    96841 gtgtttgcac cgttagcttt taaatctttc accagcgcca gcggctcatc gctgccagta
    96901 cgctcaaggg cagtcgccag agattgcacc gccgcgtagg tgatccagac ataaggcccg
    96961 gacggatctt tcttgtctgc tttcagcgca tcaacgatgc cctggtttgc cggatcctgg
    97021 tcatagcgtt ttggcatagt gaccaacatg ccttcggcag catcgccagc aatattcgac
    97081 aatgatgcgt tacccacacc ttccggcccc ataaactgag ttttcaggcc aacggaacgg
    97141 gcctggcgca gcatctgccc catttccggg tagtaaccgc cgtagtaaac gaagtcgatg
    97201 ttttcttttt tcaggcgggc gatcagcgcg gagaaatctt tctccccggc ggtaataccg
    97261 tcgaagaaga cgacgttggc gttagccgct ttcagcccgt cctgcaccga acgcgccagc
    97321 ccttcgccat actgttgttt gtcgtgaatg atggcgatgc gctggggctt caccgtctca
    97381 agaatgtatt ttgccgccgt aggcccctgg gaagagtcca gcccggcagt acgcataatg
    97441 tgttgataac cgcgttgggt cagctccggg ttggtcgctc ccggcgagat catcagaata
    97501 ccttcgtctt cgtagatatc tgacgcaggt tgggtagaag aagaacacag atgaccaata
    97561 acgtatttaa tgccgtcgtt aacgattttg ttggcgaccg caacggcttg tttcgggtcg
    97621 caggcgtcgt catattccac gcccaccagc ttatcgcctt taattccccc tttggcatta
    97681 atgtctttaa tcgcctgacg cgcgccgtta aattccatat cgccccactg ggcaatcggg
    97741 ccggacatcg cgccgacaac ggcaacttta atatcgtcag ccatagcggt gtgtgaaatt
    97801 gccagtgcaa tcatccctgc gatgatagtt ttcgcattcc gtttcatagt caaaaatccc
    97861 cattcgtgat gttgtgttgc tttgttttta tgtgttaaca aatcagactg ttcttttttt
    97921 atactgcact gtttttgcct gtctgattaa gggtttagcg cagtattttg tgataatagc
    97981 gattaaaatc ccta
//
LOCUS       DAJOWB010000003        95565 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJOWB010000003 DAJOWB010000000
VERSION     DAJOWB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 95565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.003"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            49..567
                     /locus_tag="OHO85_000212"
     CDS             49..567
                     /locus_tag="OHO85_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCQ2669645.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            583..1122
                     /locus_tag="OHO85_000213"
     CDS             583..1122
                     /locus_tag="OHO85_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCQ2669646.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1217..2794)
                     /gene="guaA"
                     /locus_tag="OHO85_000214"
     CDS             complement(1217..2794)
                     /gene="guaA"
                     /locus_tag="OHO85_000214"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCQ2669647.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2863..4329)
                     /gene="guaB"
                     /locus_tag="OHO85_000215"
     CDS             complement(2863..4329)
                     /gene="guaB"
                     /locus_tag="OHO85_000215"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCQ2669648.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4491..5861
                     /gene="xseA"
                     /locus_tag="OHO85_000216"
     CDS             4491..5861
                     /gene="xseA"
                     /locus_tag="OHO85_000216"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCQ2669649.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5858..6073)
                     /locus_tag="OHO85_000217"
     CDS             complement(5858..6073)
                     /locus_tag="OHO85_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCQ2669650.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6142..7614)
                     /gene="der"
                     /locus_tag="OHO85_000218"
     CDS             complement(6142..7614)
                     /gene="der"
                     /locus_tag="OHO85_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCQ2669651.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7732..8910)
                     /gene="bamB"
                     /locus_tag="OHO85_000219"
     CDS             complement(7732..8910)
                     /gene="bamB"
                     /locus_tag="OHO85_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCQ2669652.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8921..9541)
                     /gene="yfgM"
                     /locus_tag="OHO85_000220"
     CDS             complement(8921..9541)
                     /gene="yfgM"
                     /locus_tag="OHO85_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCQ2669653.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9559..10833)
                     /gene="hisS"
                     /locus_tag="OHO85_000221"
     CDS             complement(9559..10833)
                     /gene="hisS"
                     /locus_tag="OHO85_000221"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCQ2669654.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10944..12062)
                     /gene="ispG"
                     /locus_tag="OHO85_000222"
                     /gene_synonym="gcpE"
     CDS             complement(10944..12062)
                     /gene="ispG"
                     /locus_tag="OHO85_000222"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCQ2669655.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12089..13102)
                     /gene="rodZ"
                     /locus_tag="OHO85_000223"
     CDS             complement(12089..13102)
                     /gene="rodZ"
                     /locus_tag="OHO85_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCQ2669656.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13387..14541)
                     /gene="trmG/rlmN"
                     /locus_tag="OHO85_000224"
     CDS             complement(13387..14541)
                     /gene="trmG/rlmN"
                     /locus_tag="OHO85_000224"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCQ2669657.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14691..15122)
                     /gene="ndk"
                     /locus_tag="OHO85_000225"
     CDS             complement(14691..15122)
                     /gene="ndk"
                     /locus_tag="OHO85_000225"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCQ2669658.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15271..17583)
                     /gene="pbpC"
                     /locus_tag="OHO85_000226"
     CDS             complement(15271..17583)
                     /gene="pbpC"
                     /locus_tag="OHO85_000226"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCQ2669659.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDHWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAKLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17584..22545)
                     /gene="yfhM"
                     /locus_tag="OHO85_000227"
     CDS             complement(17584..22545)
                     /gene="yfhM"
                     /locus_tag="OHO85_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCQ2669660.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGLTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAGFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDTPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPDETVADQQKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22752..23597
                     /gene="sseA"
                     /locus_tag="OHO85_000228"
     CDS             22752..23597
                     /gene="sseA"
                     /locus_tag="OHO85_000228"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCQ2669661.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQVIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(24090..24866)
                     /gene="sseB"
                     /locus_tag="OHO85_000229"
     CDS             complement(24090..24866)
                     /gene="sseB"
                     /locus_tag="OHO85_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCQ2669662.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQVAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25008..26291)
                     /gene="pepB"
                     /locus_tag="OHO85_000230"
     CDS             complement(25008..26291)
                     /gene="pepB"
                     /locus_tag="OHO85_000230"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCQ2669663.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLRDQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26350..26550)
                     /gene="iscX"
                     /locus_tag="OHO85_000231"
     CDS             complement(26350..26550)
                     /gene="iscX"
                     /locus_tag="OHO85_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCQ2669664.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26562..26897)
                     /gene="fdx"
                     /locus_tag="OHO85_000232"
     CDS             complement(26562..26897)
                     /gene="fdx"
                     /locus_tag="OHO85_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCQ2669665.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(26899..28749)
                     /gene="hscA"
                     /locus_tag="OHO85_000233"
     CDS             complement(26899..28749)
                     /gene="hscA"
                     /locus_tag="OHO85_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCQ2669666.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVMATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEASEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGNDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28766..29281)
                     /gene="hscB"
                     /locus_tag="OHO85_000234"
     CDS             complement(28766..29281)
                     /gene="hscB"
                     /locus_tag="OHO85_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCQ2669667.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWAAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29377..29700)
                     /gene="iscA"
                     /locus_tag="OHO85_000235"
     CDS             complement(29377..29700)
                     /gene="iscA"
                     /locus_tag="OHO85_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCQ2669668.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29717..30103)
                     /gene="iscU"
                     /locus_tag="OHO85_000236"
     CDS             complement(29717..30103)
                     /gene="iscU"
                     /locus_tag="OHO85_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCQ2669669.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30131..31345)
                     /gene="iscS"
                     /locus_tag="OHO85_000237"
     CDS             complement(30131..31345)
                     /gene="iscS"
                     /locus_tag="OHO85_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCQ2669670.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31457..31945)
                     /gene="iscR"
                     /locus_tag="OHO85_000238"
     CDS             complement(31457..31945)
                     /gene="iscR"
                     /locus_tag="OHO85_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCQ2669671.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(32215..32955)
                     /gene="trmJ"
                     /locus_tag="OHO85_000239"
     CDS             complement(32215..32955)
                     /gene="trmJ"
                     /locus_tag="OHO85_000239"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCQ2669672.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            33074..33877
                     /gene="suhB"
                     /locus_tag="OHO85_000240"
     CDS             33074..33877
                     /gene="suhB"
                     /locus_tag="OHO85_000240"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCQ2669673.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34022..34876
                     /locus_tag="OHO85_000241"
     CDS             34022..34876
                     /locus_tag="OHO85_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCQ2669674.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGRGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35067..36347
                     /gene="csiE"
                     /locus_tag="OHO85_000242"
     CDS             35067..36347
                     /gene="csiE"
                     /locus_tag="OHO85_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCQ2669675.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36339..37478)
                     /locus_tag="OHO85_000243"
     CDS             complement(36339..37478)
                     /locus_tag="OHO85_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCQ2669676.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37638..38528)
                     /gene="hcaR"
                     /locus_tag="OHO85_000244"
     CDS             complement(37638..38528)
                     /gene="hcaR"
                     /locus_tag="OHO85_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCQ2669677.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38664..40025
                     /locus_tag="OHO85_000245"
     CDS             38664..40025
                     /locus_tag="OHO85_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCQ2669678.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40022..40540
                     /locus_tag="OHO85_000246"
     CDS             40022..40540
                     /locus_tag="OHO85_000246"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCQ2669679.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40540..40860
                     /locus_tag="OHO85_000247"
     CDS             40540..40860
                     /locus_tag="OHO85_000247"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCQ2669680.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            40857..41669
                     /gene="hcaB"
                     /locus_tag="OHO85_000248"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             40857..41669
                     /gene="hcaB"
                     /locus_tag="OHO85_000248"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCQ2669681.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYAMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41679..42881
                     /locus_tag="OHO85_000249"
     CDS             41679..42881
                     /locus_tag="OHO85_000249"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCQ2669682.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            42978..43400
                     /locus_tag="OHO85_000250"
     CDS             42978..43400
                     /locus_tag="OHO85_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCQ2669683.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43448..44320)
                     /locus_tag="OHO85_000251"
     CDS             complement(43448..44320)
                     /locus_tag="OHO85_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCQ2669684.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44332..45426)
                     /locus_tag="OHO85_000252"
     CDS             complement(44332..45426)
                     /locus_tag="OHO85_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCQ2669685.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45459..46457)
                     /locus_tag="OHO85_000253"
     CDS             complement(45459..46457)
                     /locus_tag="OHO85_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2669686.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46482..47993)
                     /locus_tag="OHO85_000254"
     CDS             complement(46482..47993)
                     /locus_tag="OHO85_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCQ2669687.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(48016..48999)
                     /locus_tag="OHO85_000255"
     CDS             complement(48016..48999)
                     /locus_tag="OHO85_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCQ2669688.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAAEKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVLAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49096..52377)
                     /locus_tag="OHO85_000256"
     CDS             complement(49096..52377)
                     /locus_tag="OHO85_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCQ2669689.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTPEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPERAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52495..53688
                     /locus_tag="OHO85_000257"
     CDS             52495..53688
                     /locus_tag="OHO85_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCQ2669690.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWM
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(53761..55014)
                     /gene="glyA"
                     /locus_tag="OHO85_000258"
     CDS             complement(53761..55014)
                     /gene="glyA"
                     /locus_tag="OHO85_000258"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCQ2669691.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55342..56532
                     /gene="hmpA"
                     /locus_tag="OHO85_000259"
     CDS             55342..56532
                     /gene="hmpA"
                     /locus_tag="OHO85_000259"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCQ2669692.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKQEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56577..56915)
                     /gene="glnB"
                     /locus_tag="OHO85_000260"
     CDS             complement(56577..56915)
                     /gene="glnB"
                     /locus_tag="OHO85_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCQ2669693.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(56976..58310)
                     /gene="glrR"
                     /locus_tag="OHO85_000261"
     CDS             complement(56976..58310)
                     /gene="glrR"
                     /locus_tag="OHO85_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCQ2669694.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58300..59013)
                     /gene="qseG"
                     /locus_tag="OHO85_000262"
     CDS             complement(58300..59013)
                     /gene="qseG"
                     /locus_tag="OHO85_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCQ2669695.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(59178..60605)
                     /gene="qseE"
                     /locus_tag="OHO85_000263"
     CDS             complement(59178..60605)
                     /gene="qseE"
                     /locus_tag="OHO85_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCQ2669696.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61163..65050)
                     /gene="purL"
                     /locus_tag="OHO85_000264"
                     /gene_synonym="purI"
     CDS             complement(61163..65050)
                     /gene="purL"
                     /locus_tag="OHO85_000264"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCQ2669697.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAIAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQMLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65308..66864
                     /gene="mltF"
                     /locus_tag="OHO85_000265"
     CDS             65308..66864
                     /gene="mltF"
                     /locus_tag="OHO85_000265"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCQ2669698.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(66861..67397)
                     /gene="tadA"
                     /locus_tag="OHO85_000266"
     CDS             complement(66861..67397)
                     /gene="tadA"
                     /locus_tag="OHO85_000266"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCQ2669699.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(67422..68057)
                     /gene="yfhb"
                     /locus_tag="OHO85_000267"
     CDS             complement(67422..68057)
                     /gene="yfhb"
                     /locus_tag="OHO85_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCQ2669700.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68266..69114
                     /locus_tag="OHO85_000268"
     CDS             68266..69114
                     /locus_tag="OHO85_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCQ2669701.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            69170..69430
                     /gene="yfhL"
                     /locus_tag="OHO85_000269"
     CDS             69170..69430
                     /gene="yfhL"
                     /locus_tag="OHO85_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCQ2669702.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(69624..69704)
                     /gene="shoB"
                     /locus_tag="OHO85_000270"
     CDS             complement(69624..69704)
                     /gene="shoB"
                     /locus_tag="OHO85_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCQ2669703.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(70125..70505)
                     /gene="acpS"
                     /locus_tag="OHO85_000271"
     CDS             complement(70125..70505)
                     /gene="acpS"
                     /locus_tag="OHO85_000271"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCQ2669704.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGDRLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVANMHVTLADERHYACATVIIES"
     gene            complement(70505..71236)
                     /gene="pdxJ"
                     /locus_tag="OHO85_000272"
     CDS             complement(70505..71236)
                     /gene="pdxJ"
                     /locus_tag="OHO85_000272"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCQ2669705.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(71248..71976)
                     /gene="recO"
                     /locus_tag="OHO85_000273"
     CDS             complement(71248..71976)
                     /gene="recO"
                     /locus_tag="OHO85_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCQ2669706.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGGTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(71988..72893)
                     /gene="era"
                     /locus_tag="OHO85_000274"
     CDS             complement(71988..72893)
                     /gene="era"
                     /locus_tag="OHO85_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCQ2669707.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(72890..73570)
                     /gene="rnc"
                     /locus_tag="OHO85_000275"
     CDS             complement(72890..73570)
                     /gene="rnc"
                     /locus_tag="OHO85_000275"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCQ2669708.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(73842..74816)
                     /gene="lepB"
                     /locus_tag="OHO85_000276"
     CDS             complement(73842..74816)
                     /gene="lepB"
                     /locus_tag="OHO85_000276"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCQ2669709.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGLRLSRIGGIH"
     gene            complement(74832..76631)
                     /gene="lepA"
                     /locus_tag="OHO85_000277"
     CDS             complement(74832..76631)
                     /gene="lepA"
                     /locus_tag="OHO85_000277"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCQ2669710.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(76829..77308)
                     /gene="rseC"
                     /locus_tag="OHO85_000278"
     CDS             complement(76829..77308)
                     /gene="rseC"
                     /locus_tag="OHO85_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCQ2669711.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(77305..78261)
                     /gene="rseB"
                     /locus_tag="OHO85_000279"
     CDS             complement(77305..78261)
                     /gene="rseB"
                     /locus_tag="OHO85_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCQ2669712.1"
                     /translation="MKQLWFAMSLVTGSLLFSAHASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(78261..78911)
                     /gene="rseA"
                     /locus_tag="OHO85_000280"
     CDS             complement(78261..78911)
                     /gene="rseA"
                     /locus_tag="OHO85_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCQ2669713.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(78944..79519)
                     /gene="rpoE"
                     /locus_tag="OHO85_000281"
     CDS             complement(78944..79519)
                     /gene="rpoE"
                     /locus_tag="OHO85_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCQ2669714.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(79516..79671)
                     /gene="rseD"
                     /locus_tag="OHO85_000282"
     CDS             complement(79516..79671)
                     /gene="rseD"
                     /locus_tag="OHO85_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCQ2669715.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            79927..81549
                     /gene="nadB"
                     /locus_tag="OHO85_000283"
     CDS             79927..81549
                     /gene="nadB"
                     /locus_tag="OHO85_000283"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCQ2669716.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(81534..82271)
                     /gene="trmN"
                     /locus_tag="OHO85_000284"
     CDS             complement(81534..82271)
                     /gene="trmN"
                     /locus_tag="OHO85_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCQ2669717.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWVTQQTARFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            82403..83737
                     /gene="srmB"
                     /locus_tag="OHO85_000285"
     CDS             82403..83737
                     /gene="srmB"
                     /locus_tag="OHO85_000285"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCQ2669718.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(83946..84827)
                     /locus_tag="OHO85_000286"
     CDS             complement(83946..84827)
                     /locus_tag="OHO85_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2669719.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            84930..85517
                     /gene="eamB"
                     /locus_tag="OHO85_000287"
     CDS             84930..85517
                     /gene="eamB"
                     /locus_tag="OHO85_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCQ2669720.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQQLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(85573..85956)
                     /gene="grcA"
                     /locus_tag="OHO85_000288"
     CDS             complement(85573..85956)
                     /gene="grcA"
                     /locus_tag="OHO85_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCQ2669721.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            86261..86950
                     /gene="ung"
                     /locus_tag="OHO85_000289"
     CDS             86261..86950
                     /gene="ung"
                     /locus_tag="OHO85_000289"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCQ2669722.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(86998..88035)
                     /locus_tag="OHO85_000290"
     CDS             complement(86998..88035)
                     /locus_tag="OHO85_000290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCQ2669723.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            88242..88661
                     /gene="trxC"
                     /locus_tag="OHO85_000291"
     CDS             88242..88661
                     /gene="trxC"
                     /locus_tag="OHO85_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCQ2669724.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            88730..89428
                     /gene="tapT"
                     /locus_tag="OHO85_000292"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             88730..89428
                     /gene="tapT"
                     /locus_tag="OHO85_000292"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCQ2669725.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            89460..92120
                     /gene="pat"
                     /locus_tag="OHO85_000293"
     CDS             89460..92120
                     /gene="pat"
                     /locus_tag="OHO85_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCQ2669726.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            92234..93589
                     /gene="pssA"
                     /locus_tag="OHO85_000294"
     CDS             92234..93589
                     /gene="pssA"
                     /locus_tag="OHO85_000294"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCQ2669727.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            93635..93958
                     /locus_tag="OHO85_000295"
     CDS             93635..93958
                     /locus_tag="OHO85_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCQ2669728.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(93955..95253)
                     /gene="kgtP"
                     /locus_tag="OHO85_000296"
     CDS             complement(93955..95253)
                     /gene="kgtP"
                     /locus_tag="OHO85_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCQ2669729.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
BASE COUNT        22781 a        26040 c        24025 g        22719 t
ORIGIN      
        1 tgatgatctt aaatgtctta tttttcgtaa tgtgtataac aaggaatagt gatgaaattt
       61 aaaaaatgtc ttctgcctgt ggcaatgtta gcgtcattca ctctggcagg atgccagtca
      121 aatgctgacg atcatgccgc cgatgtttat caaaccgatc aactgaatac caaacaagaa
      181 actaaaaccg ttaatattat ttccattctt cccgcaaaag ttgccgtaga caactcccaa
      241 aataaacgga acgcacaagc cttcggcgcg cttattggcg cagtcgctgg cggtgttatc
      301 ggccacaacg tcggttctgg cagcaattcc ggaacgacgg caggggcagt tggcggcgga
      361 gctgtaggcg cggcagcggg ttctatggtg aatgataaaa ccttagtgga aggtgtttct
      421 ttaacatata aggaaggcac caaagtgtat acctctaccc aggtgggtaa agagtgccag
      481 tttacgacag gtttagccgt tgttattacc acaacgtata acgaaacgcg tattcagcca
      541 aatactaaat gtcctgaaaa gagctaataa tcaggaggag tcatgaagaa agtttttctt
      601 tgcgccatct tggcctcctt aagctatccg gctatcgcct catcattgca ggatcaactc
      661 tctgctgtag cagaagctga acagcaaggt aaaaatgaag agcaaaggca gcatgacgaa
      721 tgggtcgcgg agcgcaacag ggaaatccag caagagaagc aacgtcgcgc aaatgcccag
      781 gccgccgcta acaaaagagc ggcaacggca gcagcaaata agaaagctcg tcaggataaa
      841 ctggacgccg aagcctctgc ggacaaaaaa cgcgatcaaa gttatgaaga tgagctacgc
      901 agcttagaga ttcagaaaca aaaactggcg ctggcgaaag aagaagcccg cgttaagcga
      961 gaaaacgaat ttatcgatca ggaactgaag cacaaagctg cgcaaaccga tgtggtgcaa
     1021 tctgaagctg acgccaacag aaatatgact gaaggcggtc gcgatctgat gaaaagcgtg
     1081 ggcaaagcag aagagaacaa atcggacagc tggtttaatt aatcgatgtt agtaacttca
     1141 agcctatgat tcttgaagat aaaaaaccct ctgtagtaac agagggtttt gttcattcat
     1201 agtgcaggga ttaaaatcat tcccactcaa tggtagctgg cggcttgccg ctgatgtcat
     1261 acaccacgcg ggaaataccg ttcacttcat tgataatgcg gttggaaacg cgaccgagga
     1321 aatcgtacgg cagatgcgcc cagtgtgcgg tcataaagtc gatggtttcg acagcacgca
     1381 gagagacaac ccagtcatac ttacgaccat cgcccattac gccaacggaa cgtaccggca
     1441 ggaacacagt gaacgcctgg ctgactttgt cgtacaggtc cgctttacgc agttcttcaa
     1501 tgaagatggc gtcagcacgg cgcagcaggt cacagtactc tttcttcact tcacccagca
     1561 cacgaacgcc aaggcctggt cccgggaacg ggtgacggta cagcatgtcg tacggcaggc
     1621 ccagctccag accaatctta cgcacttcgt ctttgaacag ctctttcagc ggttcaacca
     1681 ggcccatctt catctctttc ggcaggccgc ccacgttgtg gtgagatttg atgacgtgtg
     1741 ctttaccggt tgcagaagcc gcagattcga taacgtcagg gtagatggtg ccctgcgcca
     1801 gccacttcac gtcttccagt ttcagcgctt cttcatcgaa tacttcaacg aaaacgcgcc
     1861 cgatgatttt acgttttgct tccggatcgt tttcgccagc cagcgctgac aggaagcgat
     1921 cttctgccgg tacgtgaaca atgttaagac caaagtgatc gccaaacata tccagaacct
     1981 gctctgcttc gttgagacgc agcaggccgt tgtcgacgaa tacgcaagtc aggtttttac
     2041 cgatagcgcg gtgcagcagc attgcggtta cggaggaatc cacaccacca gagaggccga
     2101 ggatgacttt atcgtcgccg acctgctcgc ggatgcgagc tacagcatcg tcgataattt
     2161 tcgctggcgt ccacagggct tcacactggc agatatcacg cacaaaacgc tccagcatgc
     2221 gcataccctg gcgggtgtga gtcacttccg ggtggaactg tacgccatag aagcgttttt
     2281 cttcgttagc cataatggca aacgggcagc tttcggtgct ggctacggtg acgaagtcgg
     2341 acgggatagc ggtaacttta tcgccgtggc tcatccagac atcgagcagc ggtttaccgt
     2401 ctgcggtcag cgcatcttcg ataccgcgaa ccagtgcgct gtcgtttacg acttcaacct
     2461 gcgcgtagcc aaattcacgt tcgttagagg cttcaacgtg accgcccaac tgcattgcca
     2521 tggtctgcat gccatagcaa acgccgaata ccggtacgcc tgcttcaaag acatactgcg
     2581 gcgcacgcgg gctgttttct tcggtggtgc tttccgggcc gccggaaaga ataatgccgc
     2641 ttggattgaa gtcacgaatt tgtgcttctg tcacatccca cgcccacagt tcgcagtaaa
     2701 cacccagctc acgcacgcgg cgcgcaacca gttgagtgta ctgagaaccg aagtccagaa
     2761 tgaggatgcg atgcttatga atgttttccg tcattgacgc ttattccgag gcaagtgaaa
     2821 cagataatat aaatcgcccg acatgaagtc gggcgaagag aatcaggagc ccagacggta
     2881 gttcggggac tctttagtaa tggtcacgtc gtgaacgtgg ctttcctgaa tgcccgcacc
     2941 gctgatacgt acaaactccg ctttagtacg cagttcgtcg atagtaccac agccggtcag
     3001 acccatacag gagcgcaggc cgcccatctg ctggtgaatg atctctttca ggcgaccttt
     3061 ataagctacg cgaccttcga taccttccgg caccagtttg tcggcagcgt tatcgctctg
     3121 gaaataacgg tcagaggaac ctttggacat cgcgcccagg gaacccatac cacggtaaga
     3181 tttgtaagaa cggccctggt agagttcgat ttcacccgga gattcttcag tacccgccag
     3241 catggaacct accatcaccg cgcttgcgcc agcggcgata gctttggcga tgtcgccgga
     3301 gaagcgaata ccaccatctg cgataaccgg aataccggtg ccttccaggg cttcaactgc
     3361 gtcagcaacg gcagtgatct gcggaacacc tacgccagta acgatacgag tcgtacagat
     3421 agaaccaggg ccgataccga ctttaaccgc gctgcaacca gcttctgcca aagcgcgtgc
     3481 acctgcagct gttgccacgt tgccgccgat gatttgcaga tccggatatt tagcacgggt
     3541 ttcacggata cgctgcaaaa cgccttctga gtgaccgtgg gaggagtcga tcagcagaac
     3601 gtcaacgcca gcggcaacca gcgcgtcaac acgctcttcg ttacccgcac ctgcgccaac
     3661 tgcagcacca acacgcagac ggccttgctc gtctttacag gcgttcggtt tacgttccgc
     3721 tttctggaag tctttcacgg tgatcatgcc gatcaggtgg aattcgtcat caaccaccag
     3781 cgctttttca acgcgttttt cgtgcatttt tgccagcacc acttcacggg cttcaccttc
     3841 acgcacggtg accagacgct ctttcggcgt catgtaaacg ctaaccggct ggttcaggtc
     3901 ggtaacaaaa cgcacgtcac gaccggtgat aatacccacc agttcgtttt cttcggtaac
     3961 gaccggatag cccgcaaaac cgttacgctc agtcagttct ttcacttcgc gcagcgtcgt
     4021 ggttggcaga acagtctgcg gatcagtcac cacaccagat tcgtgttttt tcacacggcg
     4081 aacttcttct gcctggcgtt caatggacat gtttttgtgg ataaagccga taccgccttc
     4141 ctgagccaga gcaatagcca ggcgcgcttc cgttacggta tccattgctg cggaaagcat
     4201 agggatattc agacgaatag ttttcgtcag ctgggtgctg aggtcagcag tattcggcag
     4261 aacggtagaa tgagcaggaa cgaggagaac gtcgtcaaac gtcagagctt ctttagcgat
     4321 acgtagcatg ggcaatatct cgaccagagt ggttaataaa tattgccgcg gcattataca
     4381 gagcgtaacc gattgcatct accccttttt gcaaaaaatg cttgctatcc ccgaagggtg
     4441 ggttactatc gactgaataa cctgctgatt tagaatttga tctcgctcac atgttacctt
     4501 ctcaatcccc tgcaattttt accgttagtc gcctgaatca aacggttcgt ctgctgcttg
     4561 agcatgagat gggacaggtt tggatcagcg gcgaaatctc taatttcaca caaccggctt
     4621 ccggtcactg gtactttaca ctcaaagacg acaccgccca ggtacgctgc gcgatgttcc
     4681 gcaacagcaa ccgccgggtg accttccgtc cacagcatgg acaacaagtt ttagttcgcg
     4741 ccaatattac gctctacgag ccgcgcggtg actaccagat aatcgttgag agtatgcagc
     4801 cggccggtga agggctgctg caacagaagt acgaacagct caaagcgaag ttgcaggctg
     4861 aaggtttgtt cgatcagcaa tacaaaaaac cacttccctc ccctgcgcat tgcgttggtg
     4921 tgatcacctc aaaaaccggt gctgcgctac atgatatttt gcatgtgtta aaacgtcgcg
     4981 atccttctct accggtgatc atctacccca ccgccgttca gggcgatgac gcaccggggc
     5041 aaattgttcg cgccattgaa ctggcgaatc agcgcaatga gtgcgacgtg ttgatcgttg
     5101 ggcgcggcgg cggttcgctg gaagatttat ggagttttaa cgacgaacgc gtagcgcggg
     5161 cgatttttgc cagccgcatt ccggtcgtca gcgccgtcgg gcatgagacg gatgtgacca
     5221 ttgccgattt tgttgccgat ctgcgtgcgc caacgccgtc tgccgccgct gaagtagtga
     5281 gccgtaatca gcaagagtta ctgcgccagg tgcaatcgac ccgtcaacgg ctggagatgg
     5341 cgatggatta ttatctcgcc aaccgcacgc gtcgctttac gcagatccat caccgattac
     5401 agcaacagca tccgcagctc cggctggcac gccagcaaac catgcttgag cgcctgcaaa
     5461 agcggatgag ctttgcgctg gaaaatcaac ttaagcgtac cgggcaacag cagcagcggt
     5521 taacacagcg gctgaatcag caaaatccac agccgaagat tcatcgcgcg caaacgcgca
     5581 ttcagcaact ggaatatcgt ttagcagaaa ccctgcgcgt acagcttagc gccacgcgtg
     5641 aacgtttcgg taatgcagta acgcacctcg aagccgtaag cccactgtca acgctggcgc
     5701 gtggatacag cgttactact gctactgacg gcaatgtact gaaaaaagtg aagcaagtta
     5761 aagcgggtga aatgctaacc acacgtctgg aagacggctg gatagaaagt gaagtaaaaa
     5821 acatccagcc ggtaaaaaaa tcgcgtaaaa aggtgcatta agccagcacg aattcaacgc
     5881 gttttttcga tatcaagcca tgcccgtgct gacagaagta atctactgca ccgcaggctt
     5941 ttaacacctg caacggttga tggcagtctg gacaaagagc tttcatttca ataatttccc
     6001 cgcaggatgg gcaacgcgca tggccgttat cctgatcaag cacatgctga cattgtggac
     6061 aatgcagttc catatgaatt cctccggtag ccatccctct acattcatag agggaatggc
     6121 agataaaata cttacggata attatttatt tttcttgatg tgcttcatca gacgcttacg
     6181 tttacgcatc tgggttggcg tcagggtgtt acgcttgttc gcatacgggt tttccccttc
     6241 tttgaactga atacgaatcg gcgatcccat tacgtccagc gatttgcgga agtagttcat
     6301 caagtagcgc ttgtaggaat caggcaggtc tttcacctga ttaccgtgaa tcaccacaat
     6361 cggcgggtta taaccaccag cgtgggcata tttcagcttc acacgacgac cgcgtaccag
     6421 cggcggttgg tgatcttcaa cagccatcgt catgatgcgc gtcagcatag aggtccccac
     6481 acgacgggtg gagctgtcat acgcttcacg tactgattca aacaagttac caacaccact
     6541 gccgtgcaag gcagagataa agtgcacacg agcaaaatcg ataaagccca gacggaagtc
     6601 cagcgtttct ttcacctgct ctttcacttc ctggctcagg ccatcccact tattcaccac
     6661 aatgacaagt gaacgcccac tattgagaat aaagcccagc agcgagagat cctgatcgga
     6721 aataccttcg cgcgcatcaa tcactaacat caccacgttg gcgtcttcaa tggcctgcaa
     6781 cgttttgatt acggaaaatt tctctacagc atcggtgatt ttgccgcgtt tacgtacgcc
     6841 agcggtgtca atgagcacat actcacgtcc atcgcgttcc attgggatgt agatgctgtc
     6901 acgcgtcgtg ccaggcatgt cataaacaac aacgcgctct tcaccaagaa tacggttagt
     6961 gagtgtagac ttacctacgt tcggacgacc cacaatcgcc agtttgatcg gcagactttg
     7021 cgggtcgaag tcgtcttcct cttcttcttc gccgttctct tccgcttcaa attgcgccca
     7081 gtattcagcg tcttcgtcga cttcctcttg cggtgcgaga tcttccatcc acggcagcag
     7141 cacatgctcc agcagactta atacgccacg accgtgagac gcggcgatcg ggtagatttc
     7201 acctaaacca agcgagtaga aatcaaccac tgcctgatcg ggatccagac cgtcagtttt
     7261 gtttgccacc aggaaggtcg gtttttcacg ggagcgcaga tgtttggcaa tcgcttcatc
     7321 tgccggcatc aggcccgcgc gcgcatccac cataaacagt acgacgtccg cttcttcaat
     7381 cgccagcagc gactgttccg ccatgcgggt ttctacaccg tcttctgtgc catcaatccc
     7441 gccggtatca atacagataa actcacggcc ttcaatttcc gcacgaccgt acttacggtc
     7501 acgagtcaga cccgggaaat ccgcaaccag cgcatctcgg gtgcgagtta gacggttaaa
     7561 taacgtggat tttcctacgt tagggcgccc gacaagcgcg accacaggta ccatgtttaa
     7621 agcctcattt ttataaatca tcagacaacg cacgctatat tcgcgtcgtt gttaaaaaca
     7681 ggaaaacggc ccctgtccag gagccgtttt caaagtgaac gacagagacg attaacgtgt
     7741 aatagagtac acggttccgt cttttgcctg gatcagcagt ttgccgtcag cggcaaccgg
     7801 ttcagtctgg aaaccggaac tatcaacttt ttgctgggca acgaaacgac catcttcgac
     7861 gttaatccag tgcagataac cttcactgtc accgaccacc aggttgccat tatacagcac
     7921 cggagaagtc agcaggcgat gcagcagatc gctttgtgcc cacagcgtaa cgccgccatc
     7981 aatggtcaac gccatcaccc ggtcattttg atcgaccaga tagatgcgat tgccgtcgac
     8041 gatgaaatca ttcaccgaac ccagttcgcg tttccacata atctgaccac tgcgcagatc
     8101 aagcgccgtc aggttaccat tataggccag cgcgaaaaca acgccgttaa caacgacggg
     8161 agtcgtgtca acatcgctca gacggtcaat ttcggtagaa ccggtcgcct gggaaatacg
     8221 ctgctgccaa atcatctggc cctgttccat cagcactgcg ctgacgcgac cattatcgcc
     8281 ccccacgacg gccgcaccaa aagccgttgc cggcgcagac tcgccacgca aagagagcga
     8341 aggcatatcg aggttaactg tccatttgac agcgccgtca gcttcgttta gcgcttgtaa
     8401 ctgaccgtta ctggtgtgga ttaacaccag accgtcgctg accaccgggc gcgaaagtgc
     8461 ttcacccgcg actttagttt gccatgccac agtaccatcg ctggtattca gcgcgtaaac
     8521 ctgcgccttt tcgctgccaa tgtagacatg cccaccagac acggtcacac cgccagaaag
     8581 taatgcaggc tctttagaga accagccatc tttctcggcc aggctgacag accagatttc
     8641 tttgccatca tccgcattca gcgcttttac caaaccagcg cggtccgctg catagacaac
     8701 gttgtccgcc agtgccggat gaagattgga atagaagttg ccaatgccgc taccaacgga
     8761 agtactccac gccgtggtcg gcgtaaactg gttttcaacg gttggcaatg gggacatctt
     8821 taccacatct tcttcgctgt taaacagcga acagccgctt aaaagggtaa cggaaagcag
     8881 tcctggcagc agtaatttac gcaattgcat cgggtccctc tcagatggac aaattattaa
     8941 ttttcatctg catcatttcg ctcagtgccg gagtaacatc gcttttcacg cctgcttccc
     9001 atgcactacg cgcaccttgc ttatcacctt tgctcagcaa tgcttcacca cgcaggtcgg
     9061 caacaatggc agcccaccct tcacctttga tggtatcaag ggttttcagc gcggcatcag
     9121 cctgcttgag ctgtacctga acgcgagcaa gacgcagatt tatcacggct ttgagatttt
     9181 catcgctcgt gtctgccagc ccctgttgta actgggcggc agctttctct agttcatttt
     9241 tgtcaacaaa ttgctgcgca agttccaaag aagccagcgc accataagta tttttatttt
     9301 cagcagcaaa tttttccgcc gccgggatgc tatccggttt gccttcgctc actgcggtaa
     9361 ccgcattttg ataggcaaga gaagcggagc gtgcagaatc aacctgatgg ctgttccagt
     9421 agcgccagcc aatcagtgcg ccaacgccca aaatcacccc aacagccagt gctttgccat
     9481 tttcagcaaa aaagcgttta accgcttcta cctggtcgtt ttcgttctcg taaatttcca
     9541 cgctgtcctt ctccttcctt aacccagtaa cgtgcgcaaa tgcgcggcta cgctatcctg
     9601 cgcaactgcc gtttgctcac cagagcgcaa atccttcact actgctgtgc cgttagccac
     9661 ttcagactca cccagcacca cagcaacgcg ggcaccccat ttatcagcac gggcaaactg
     9721 tttcttaaag ttgccgccgc cgtggttggt catcaatttc acgcccggta attcatcacg
     9781 cagacgctca gctaatgcca tagccgcaga ttgtgtatca gcacctgaag ccaccaggta
     9841 tatatcgaca acaggatcgg ctttaaattc cggattaacg gcctgtacta acaatacaag
     9901 acgttcgagg cccatcgcaa aaccgacagc cggtgttgca cgaccgccca gttgttccac
     9961 aagaccgtca taacgaccgc ctgcacacac ggtgccctgg gagccgagac tgttagtcac
    10021 ccactcgaaa acggtacggt tatagtaatc cagaccacgc accagacgct ggtttacggt
    10081 gtaagcgatc cccgcgctct caagcagttt gcacagaccg gcaaaatgct cacgagattc
    10141 ctcgtccaga taatcaccta atgccggagc gtcgttgaga agcgcctgca cttccggatt
    10201 ttttgaatcc agcacgcgca gcgggttagt gtacatgcgg cgtttgcagt cttcgtccag
    10261 cttttcttta tgctgctcaa ggaatgccac cagcgcatcg cggtaattgg cgcgtgcttc
    10321 cagcgaaccg atagaattca gctcaagagt tacgtgttcg gaaataccca gcgcgcgcca
    10381 ccagcgggcg gtgagcataa tcagttcagc gtcgatatcc ggaccttgca gaccgaaaac
    10441 ttcgcagccc aactgatgga actgacgata acgccctttc tgcggacgct cgtgacggaa
    10501 catcggcccg atataccaca gacgctgttc ctgattgtac agaagaccat gctcgatgcc
    10561 ggcgcgtaca cagcccgccg tcccttcagg gcgcagagtc aggctgtcgc cattgcgatc
    10621 ctcaaaggtg tacatctctt tttcaaccac gtcggtgact tcaccaatcg cacgtttgaa
    10681 tagcggggtc tgctctacaa tcggcaagcg gatttcactg taaccgtagc tgccgagcac
    10741 gtttttcagt gtgccttcaa tgcgctgcca gatggccgtt tcgccaggca ggtaatcgtt
    10801 catgccgcga atggcttgaa tgttttttgc cacgtttatt ctctttctga atataaaaat
    10861 gaaccctcaa cgcttccctc aatgtttcgg gagctatgcg ggttcaatca tacacgggaa
    10921 gcgaggcgct tcccatcacg ttattatttt tcaacctgct gaacgtcaat tcgacgcgct
    10981 tcgtccagct gactggcttt cgcacgaatg cgtgcttcca gctggtcgat catatcgttg
    11041 ttgtccagac ggtctttgcg cacgccatct tcatagaggc cgcttttctt gttgccgccg
    11101 gtgacgccga gtgtagaaac cagcgcctca cctgggccat tcaccacgca gccgataatc
    11161 gaaacgtcca tcggagtgat gatatcttcc aggcgttgct ccagcgcgtt aaccgtaccg
    11221 ataacatcaa attcctgacg cgaacaggtc gggcaggcga tgaagttgat acctcgcgaa
    11281 cggatacgca gcgatttcaa aatatcgaaa ccgactttga tctcttcgac cggatcggcc
    11341 gccagtgata cgcgcagcgt gtcgccgatg ccttcagaca gcagcagacc taaaccaatg
    11401 gcggatttta ctgccccgct gcgtgcacca ccggcttcgg tgatccccag atgcagcggc
    11461 tgatcgatct gttttgccag caaacgataa gactcaacag cgaggaagac gtcagacgct
    11521 ttcacgctga ctttgaactg atcgaagttc aggcgatcga gatgatcaac atggcgcatg
    11581 gcggattcca gcaacgcctg cggcgtcggt tcgccatact tttcttgcag atctttttcc
    11641 agcgatccgg cgttaacgcc aatacggatc ggaatgtttt tatcgcgcgc acagtcaacc
    11701 accatgcgaa tacgctcttc attaccgata ttgccagggt taatacgcag acaatcgacg
    11761 ccgtattccg ctactttcag tgcaatgcga tagtcgaagt ggatgtcagc caccagcggc
    11821 acgttaacct gctgtttgat gagtttgaac gcttctgccg cgtccatcgt cggtacggag
    11881 acacggacga tatcagcgcc aacgcgttcc agcgccttga tttgattgac cgttgcttcg
    11941 acgtctgtcg tacgcgtatt ggtcatggac tgtacggcga tgggagcacc atcgccaatc
    12001 ggcacattcc caacgtaaat acgtgttgat tttctacgtt gaattggagc ctggttatgc
    12061 atgaaaaatc tcccgcgtta cccgtctgtt actgcgccgg tgattgttcg gcattgaggg
    12121 tcagacgcgc aacctggtta gttctgataa aacgactcag atcgacaggt ttcccttgat
    12181 actggatctg tactgcggct ggcgcaccaa ttttcagttt gtatggtgcc tggccggtta
    12241 agttcaaatt accgtcttta cgctgcatac cgctaaacaa ttttttaccg gtagcatcag
    12301 taacctccag ccagcaatcg gcagtaaagt tcatcaccag cgcattcgga tcagccgccg
    12361 gcgtagtcac gcctgcctga tcggttggca acggcgcagc gccatctggc gttgttgttg
    12421 ccgttggtgc cggggtcgcg gcggtatcaa catttgcttg cgaaggcgaa acaaccgcat
    12481 tctgttgcgg atcaacagct ggtgctggcg cagttacggc aggtgtttgc gtgttggttg
    12541 cggtagtatc cacagacgct ggcggcgtgg acgttgtagc cgggtctgta gttgtcgacg
    12601 tatttaacgg aacactctgc ccctgctcgc tattactgct cagttccgcc gaagattgat
    12661 cggccatagt ggtgatctct tcctgctgag ctttgtggtc ttgccaccac caggcaccgc
    12721 tcaggccgat aaccacaaac aacaccagcc aggtgaaggt catcagccag ccgtcacgtt
    12781 ttttgcggcg tttaccgagg gaaaaactct gcataggcgc aacttttgca gcccgtagtg
    12841 gagcctgctt ttccagccct ggcagcagtt cttcttctgg aatatgtacc agacgcgcat
    12901 aagagcggat atatccgcgc aggaatgttg aagcaagatc ggcgggtgcc ttatcttctt
    12961 caatgtcgcg taccgtggaa accttcaggc aaagtcgctc ggcaacggcc tgctgactaa
    13021 gtcctagttg ttcgcgagca ttacgcaggc gagcgccggt agtaagtgct tcattttggt
    13081 cgtgcgtggc ttcagtattc attcgctgca ggtacgttta aatgagaatt gagatgccgg
    13141 tgaaccatca tgctcaccca caccgcgaaa catccgttaa gttaaccgtt atcatacagt
    13201 ataagaccgt ctgtgtgctc gtgacaaagc ccacattcac taaggctaac ttactgttgc
    13261 atcgttacat actgccttaa agtcagcaaa aacgcaccgt taatattgac cagacaattg
    13321 caacttaata cttcattcat aaaagattac gccacggtac ataaagtaac cgtggcgtaa
    13381 tggctatcag accgctttaa tgtcgatggc ttcaccctgc atccgtttac gcagggtacg
    13441 tttcgtacgg tcgataacat cgcccgccaa ctgaccacag gcagcatcga tatcatcacc
    13501 acgggtttta cgcacaatag tggtgaaacc gtagctcatc agcacttttg agaaacggtc
    13561 gatacggctg ttcgagctgc gtccatacgg cgcgcccggg aaggggttcc acgggatcag
    13621 gttgatctta cacggcgtat ctttcagcag ttccgccagt tggtgcgcgt gttcagtgcc
    13681 gtcgttaacg tggtcaagca tcacatactc aatagtgaca cggccctgat tggcgttgga
    13741 tttctccaga taacggcgca ccgcagcaag gaacgtttcg atattgtact ttttgttgat
    13801 cggcacaatt tcgtcacgaa tttcgtcgtt cggcgcgtgc agggaaattg ccagtgcaac
    13861 gtcgatcata tcgcccagtt tatccagcgc cggaactaca ccggaagtag aaagcgtgac
    13921 gcgacgttta gacaggccaa aaccgaaatc atcaagcatg atttccatcg ccggaacgac
    13981 gttgttcagg ttgagcagcg gctcgcccat gcccatcatc actacgttag tgattgggcg
    14041 ctgaccggtg acttttgctg cgccgacgat tttcgccgca cgccacacct ggccaataat
    14101 ttccgatacc cgcaggttgc ggttaaagcc ctgctgggcg gtggaacaga atttacactc
    14161 cagcgcacac cccacctgcg aagagacgca gagcgtggca cggtcgtctt ccgggatata
    14221 caccgtttcg acgcgctgat cgccaacggc gatcgcccat ttaatggtgc cgtcggatga
    14281 acgctgttct tcaaccactt ccggtgcgcg gatctccgcc acctctttca gtttgccgcg
    14341 caacactttg ttgatgtcgg tcatctcatc aaagttgtcg cagcaatagt gatacatcca
    14401 cttcatcacc tgatcggcgc ggaagggttt ttcacctaaa tctttaaaaa actcccgcat
    14461 ctgctgacgg ttgagatcca gcaggttgat ttttccatct ttcgtggtga cgttttcagg
    14521 tgtgactaat tgttcagaca tatgctattc cggcctcgtt attacacgtt atggcccctg
    14581 gagggttgaa aaaagaaacg ccccggtgag cggctgctcg tccgggggcg ctgcattgta
    14641 caaattctgg cgcacggatg ccacgtttgc acgcggcatt tacgaaatta ttaacgggtg
    14701 cgcgggcaca cttcgccttc gccaaagaaa taagcgattt cgcgagcggc agattcgacg
    14761 gaatcagaac cgtgggtacc gttttcggtc aggctgtcag cgtaatcagc gcgcagagta
    14821 ccagccagtg cgtttgccgg attggtcgcg cccagcagat cgcggtgacg ctgaacggcg
    14881 ttttcacctt ccagcacgga aaccacgatc gggccagagg tcatgaattc aaccagacca
    14941 tcaaagaacg gttttccatc gtgttcagca taaaagccac gtgcctgttc aacggtcagg
    15001 tgcagcattt tggtgccgac aattttgaac cctgcagctt caaagcgcgc aaagatatta
    15061 ccaatgacgt tttttgctac cgcgttcggt ttgatgatgg aaaaagtacg ttcaatagcc
    15121 atttttacct ctgtaaattg ttctgttgtt gtctgtacca gcgtacgaga tggcgcggat
    15181 tataatgagc aacagggccg ttgactattg atgaaggtaa cattttttta aaataaaatg
    15241 agttttagca acaatcaggg gcaaaacaga ctattgcatg acaaatttca ctgtcgcgat
    15301 ttgccccaca tcatccatca ccagcaattg ataatcacct ttatccgtca aatgcagggt
    15361 cacgttgcgc ccgcgttcag ttaacggttc gccattcaga aaccaccagc gttcacctgc
    15421 ccctccactt gattgcaacg gcaaagtcgc ttccgctgcg cccggtaaac gtttaataat
    15481 cgcgccatcg cgaacgcctg tcagctgcag agggagcttg gcatcgtgac cgtatggcgg
    15541 gcaaattgtc gaggccggtg gtaagcgcac agcgcgacgt tctgatgcgg gcagccaggg
    15601 ttccagcggt agcggccaga catttatcat ttcttgtcgc gcttgcgggc aatcagcggc
    15661 aacacgtttg ccattttcat ccagccagat ggggaaacga atgccattaa tgccttcctg
    15721 ctccggcagt aatagagttg gcggctgact cccgtccagc agccaggttg ccaggcggcg
    15781 gcgacagtta ccgtcacctt ccggcaaaga ctgtccgccc ggccagcaga taacgccacg
    15841 agtgactgag ttcgggcgcg ggtcttccgg cagattcgca ctgcgcgaca gtaagatatt
    15901 attgacctga ttcaacaatg gtacggcact ggcaaagcca aactgaccaa caacgggcgt
    15961 gccgtccggt ctgccagtcc agatcccaat gacatagcga gcgttaaccc caatcgccca
    16021 ggcgtcacga tagccatagc tggtgcccgt tttccatgcc agtggggcga cgcgcggcaa
    16081 ggcactatcc ggcaagggtt tcgcttcatc agccataatc cggcgaatga tccacgccgc
    16141 ccccgacgac attaaaggcc gttcaagcag cggatcgtca ggctgtaagc gcaatttgcc
    16201 tgccttgccg tggcgagcaa acgcggtata cgctgccgcc atatcttcca gttttgcacc
    16261 agcgccgccg agaatgagtg aaagattcgg cgcagcaccg ttgggcaaat ataacggcaa
    16321 tccaacattg cgtaactttg ccgcaaaccg tttcggtcca taggcttcca gcacctgcac
    16381 agcaggtaag ttcagcgagc gcaccagcgc ctcgctcatg ctgatcgggc catgaaaacc
    16441 gctatcaaag ttacctggtc gataatcacc ggtgcgccgg gggacgtctt gcagcagtga
    16501 tgccgggtgg atcaagcctt catccagcgc cagaccataa acaaacggtt tgagcaccga
    16561 tcctggcgat cggatcgcat tgaccatatc aacatgacca aagcgtgaat catcgttgag
    16621 atcaaccgat cccacccagc cgcgaacacg catatcggta tgatcaacca cgatcatcgc
    16681 cagtgagctg cgcggtggca accgcccttt ccagttttgc gccagttctt ccagacgtcg
    16741 ttgaagaccg gcatccagcg tagtaacgat tttgtcgctt ttgcttttac cgagcatcat
    16801 gcgcgaaaac agcggtgcca gttgcggcat ttgtcggggt gccagccaga tgggttcttc
    16861 ccttgactct tttacctgct cacgggacca cacaccttgc acggccatcc gttcgagcac
    16921 tttattacgc gcggcttcgg cacgctccgg ccaacgatcc gggcgaagac ggctgggcgc
    16981 ttgcggcaaa accgccagca ttgccgcctc ggaatagctt aaattcgcag gcgactttcc
    17041 gagataagcc caacttgccg caccgatccc ctgcaacgta ccgccaaacg gagcgcggtt
    17101 aagatacaag gtcagaattt cacgcttaga cagatgccat tccagttgca acgcgcgcca
    17161 gagctggcga attttgccgc caaatgtttt ggggtgagga tcaagcagac gagcaacctg
    17221 catagtgagc gtgctgccac cggaaataac ccgtcccgaa gtgagatctt gccatgctgc
    17281 gcgcgccacc gagaatggat tcacccccgg atgcttccag aaccagtgat cttcataatt
    17341 gatcagcgct tcaaggtaac gtggagaaac atcttcgatt gttaccggat aacgccagat
    17401 gccgtcagca tcggcgaagc gccatagcgg cgtaccatcc tgcgccacga ccactcgtgc
    17461 gggattgact tcatgcagcg gtagaggcca gagtttatct gctccccacg ctgcaagaaa
    17521 aaggagaaat ggcgcagctg ccagcgttat ccagcagccg cgtttggtta acaagcgagg
    17581 catttacggt cggacaatca gcagatcttc agccgcgccg gtcgcccgcc attggggaac
    17641 atacattgat tccaccatcg gttgcggtac ctgatacgtt cccggcgtca ccgcccgcgc
    17701 cagatacacc agcgttaccg gttggtattc atcaacggca accgccgcca caaagcgatc
    17761 gtcacggaac tcaatgtgct taatactcgc ctgctgcatc tggttcagta agttctgcac
    17821 ttcgccacca ctttgctcca ggctggcgct accgttcgcc agattctgat tttccagttc
    17881 cagacccgca ggcagcagat ccacgactaa cgcatccggc acgctgttac tggcttttac
    17941 ctgcaaccag accagcacca gatcgccgct acgtaacgag tccagcgatt tgctcttacc
    18001 atcagtacca agaatatgac gctcgatttg cagcacattg ttcgccggta aaggtgcgga
    18061 ttgcggataa ccgctggcat ccatacgcaa ccataacggc tgatcgccac tgtttctcac
    18121 ctgcaaggtg gcaagttgat cgctattcag attgctgttt agcgttttct cgcctgtcag
    18181 cggctcagct gagaaagagg tttgcgcctg ccatttaccc ggtaaatcct gaatcgtacg
    18241 ggcagctagg aacaacgcgt tactttcctg cgtcgatagc cagcgttcac caaacgcctg
    18301 ctgcgaaagt gtgttcagca aagtgtactg ctcatcgggt agcagtttat tttcttccag
    18361 caaggagagc attaacgcgt tgtcgcgcag tggactaccg taatcaccca gccatatccg
    18421 ctcatcacta tttctcggcg ttttcagcgc cagcacaatc gcttcttcac cacgcgttgc
    18481 atcacccatg gttttcagcg caacgccaag ttgcagcagc ggtaaaccag aagcggcatc
    18541 tgcacgatgc tcccagattt cacgcagcgc acccagcgga gccttttgtt gacgggccaa
    18601 caccagcgca gcgtaagact gtacggcgaa tttactggct ttgagattat ccgcgtacgg
    18661 gatcgacatc atgcccggat cttgtaaata gcgcagcaga cgctcattac cccggttaat
    18721 ggcgtctgtc ggcacgctgt aaccctgttc gcctgcgcgg accaggaaat ccatcacgta
    18781 agccgtcagc cagtactctt cgtcaccgtt tttatcccac agcgcaaagc cgccgttatc
    18841 acgttgcatt tgcagcaaac gggaaatgcc gatatcgacc gatgcacggc gtttctcatc
    18901 actgtcgcct ttgatgccca acgcctgcag ttgggctgcg ttggtataaa gtgacgggaa
    18961 caggccgctg gcggtttgct caagacagcc gtacggatac gcttttaact ctttgatata
    19021 acgtgcaatg ttcagcggtg gtttgccgct caacaacaat tgcccttcca gcgtaacagg
    19081 cgagaagttt tgcaatccat ccgccggaat cgcccatgtc tcaccaggct gtaacgccgt
    19141 accgtaatta accgtttgtg ccgggaacgc cggacggacg ccgattttcc actgcttctg
    19201 ctgatcggca acggtttcat ccggtaacgc taacccgcta atggtggcct gaatttctcc
    19261 atcgccataa cctggcaatg ctcgcaccgg gataaacagc gtagtacgca cacctggcgc
    19321 taattcaacg gctgcgggtg tatcgctgac cagttcaagc aaaccactgg cggtcagggc
    19381 aacgttcagt ttttgcggtt tatcggtaag attagtgata tccagcgtca gacgcgaggt
    19441 atcgccactc gccataaagc gcggcatgtt cagttcagca atcaccggtg cggcaactat
    19501 cactttgctt tcgttgctac cgaagtcatc tgccgtccag gcttgcgcca tgacgcgtaa
    19561 ttcaccgtta aaatcgccaa tcggcagtgt aaccgagcct tcgccctgtt cgttgagcgt
    19621 taccggcagc gcctgctgcg cgacaatatt gacgtgattg accggcggtt taccaccacg
    19681 tttcagctca tcaccatcgc caccgaaacg cagagctgcc agacgcccct gaccttcaat
    19741 aacctgaccg taaatatcgt aaatgtctgc gccatagcgt ttctgaccaa agaacgcctg
    19801 ccacggatct ggcgtgacgt agtcagtaat attcagcaca ccactatcaa cggcagacac
    19861 cagcacattc acctgtttag gcttctcgcc atttttagtg ctggctttaa ttttcacggt
    19921 taatggttga ttcggccgca tttttgctgg tgtttccagc gccagatcga ggcgacggtt
    19981 ttcatcgcca agcggcagat gcaacacccc aactgcgcgt tttggcgtcg cggagcgaga
    20041 tttatcgcca ggacgtacta ccagcgtact taaatacaga tcatgacgat tccaggtttt
    20101 atcgaccgga atcgtcagat ccagcccttg agccggaaca tcaatctctt gccaccacaa
    20161 cggcccttca ctggactcga ccatcgcata acctttaccc gccgttggcg cggcaatatg
    20221 caacttaatg gtatcgccag ggcgataact ggctttatcc agtttcagcg tgacacggtc
    20281 gggtcgcacg gcaccgctcc cgtcgctgtt gtcctgccag ctatagccag cccagaaacg
    20341 cacgctactg accgcttcat tcggcgcttt gacttccaga cgataagcgc cccactctac
    20401 cgggaaactg accttaccgg tttcatccgc ctgaagatcc agagtttgtt cattttcgat
    20461 cagatctttt tgatcaaact gtgactgcca gccttcatct tctgaccagt tccagtagta
    20521 atcgcggcgt tcgcgaatca ggcgcacctg caagcccgac acggcttttt tcacgccttg
    20581 cgcatcgcta taaacgatgt caaaaccggc gttactgcct tcatcaacaa tgggctgttt
    20641 caccgtgctg tcagtgcgat aatcgtaaac cgatttcgag gcgaactgcg gacggatccc
    20701 cggcaatgca tcggcaggcc agatagcctg ctcagcgcgg cgcgtcaccg ggcgaccgcc
    20761 cgattccagc aaactaccct ggaaaataac ctgtaatggg gaatgcgttt ccttccactg
    20821 gctttctgta gaaacttcgc cgcgcccttt atcatccagc gtcaactgaa cttcatccag
    20881 cgtgcgggaa agattttcgg cagctatatc gccgaattca aaaccaggta aggctgacac
    20941 agcttcacgc agtgggcgca ggaaaagttg cccttgcaaa gtattaccat tagcaggtgc
    21001 accatacagg tagtacccca ccacggagaa tttcacttca tctttcggcg ttagcggggt
    21061 tttctcaccg gtcagattca gcgccatgcg ctctggcata aaatcttcga cgtggaaatc
    21121 ccacatccga tactggttat cgcccgtgtt agcgcgaata tgccacatac cggttgccgc
    21181 attgctatcg agtggccagg taaagtggta gaggccattc tccggctgac taacgacgct
    21241 cctgagtacc tgcccatcgg gtttaatcac gtctaacttg atgggttgat tgggcaacgc
    21301 tttaccgtct gcatcacgca gcaaaccatt gaggattacc gtttcacctg ggcgataaag
    21361 atcgcgtggg ccaaacatga aaaactgttt gctatagcct ggcgcgccag caatgttaaa
    21421 ttctgctaag tccagcgccg gaagttttaa atcgagtagc gttgtctgac cgtctttacg
    21481 cgccaacagt aatgccgcgt ttttatcatt ttccagctgc acatgcccct gagcgtcact
    21541 ggttgcctga gtcagagtca gccctttctc atttaataaa gagacttcaa ttccttgctg
    21601 ggccgcgccg ttttccagac tttgggtaaa gatatccaga cgattgtgat aacggtgagc
    21661 tgaaacgccg atatcactta acgtaaacag cgtcgcggga ttactgtaat cgtaacgtcc
    21721 agcctgattc atcacagcca gatacacgcc cgcctgctga agcggtttga tatcgcccag
    21781 cggcagcaat aatttttcac gggtgttacg cgcaggattg agatcaaacc gtccggtgta
    21841 gaccagatcc gccatctgca gcagtttgtc tgactgccag ttcgccagcg aattgcggta
    21901 ttcccattgg ctaatgaatg ctggcagaga ttctggctta acgcggaaga agttaacatc
    21961 gacattatta acgttgagcg ccattaccgg cagcccttca acgactttgc caggcagcag
    22021 cgaaccacgg ctggcaaaac cgacgctggg ttggatgtcg cgggtagtta tagttttttc
    22081 gtaatcttta ctgaaggttg cgttgttgag tgctttgacc tccttgccaa tagtaacgat
    22141 caaatcacgt ttcggttcga ggtggcgtaa acgtaactct ttaagattat ctgacagctc
    22201 ccaggcacca tccactttgc cgcttttttt atcgacgaca tgaataacgc gtgagaaatc
    22261 ctgatccggg tcgagaggga tggagaacgt cagcaccagc gtggctgcac catcaagttg
    22321 gacttcagag agatccagca atgtcagcac cttaccggca ctctgttggg ccagtttttg
    22381 tctctccggc acggagagtt ttgctgaact cgcgttttct gaagaggccg ctttagtaac
    22441 ttcagaaggc gcatcttttt tcaccgctgt tggcgcgtta tcgttgttgt cgcaccctgc
    22501 cagcgccagc attagcatgc aggcggctac gcgtaacttt ttcatttttc atccctgcca
    22561 caatggcccg ttagcaacgt cgaataatta ttatgcgtga gaatttacgt tatgtaattc
    22621 agtatcacgc ccatacaatc cgaattttag tgagaattgt ggctcagaca gccaaatcag
    22681 cctcttttgc ttgtcgcttt tcactaaaca tccgacaatt tagcctaacc ccggcaaaaa
    22741 tggagatgcc tatgtccacg acatggtttg taggagccga ctggctcgcc gaacatattg
    22801 atgacccgga aattcagatt atcgatgccc gcatggcgtc gcctggacag gaggatcgta
    22861 acgttgctca ggagtatctg aatggacata ttcccggcgc agtgtttttt gatatcgaag
    22921 cgctttctga tcacacttcc ccgcttccgc acatgctgcc gcgcccggaa acgttcgccg
    22981 tggcgatgcg tgaattaggc gttaaccagg ataagcacct gattgtctat gacgaaggta
    23041 atcttttctc agccccacga gcatggtgga tgctgcgcac ctttggtgta gagaaagtgt
    23101 cgattctggg gggtggactt gcaggctggc agcgtgatga tctgctgtta gaagaaggtg
    23161 cagtagagct gccggaagga gagtttaacg ccgcatttaa tcctgaagcc gtggtgaaag
    23221 taaccgatgt attattggca agccatgaaa atacggcgca agttattgat gcccgcccgg
    23281 ctgcacgttt taacgcagaa gttgatgaac ctcgcccagg tttacgtcgc ggacatattc
    23341 ccggtgcatt gaacgttccg tggacggaac tggtgcgcga aggcgaacta aaaacgaccg
    23401 atgaactgga tgcgatattt tttggtcgcg gcgtcagcta cgacaaacca attatcgtca
    23461 gctgcggctc tggtgtaacg gcagccgtgg ttttgttggc actcgcgacg ctggatgtgc
    23521 caaacgtgaa actgtacgac ggcgcatgga gtgaatgggg cgcacgggca gatttaccgg
    23581 ttgagccagt gaaataagct ttatacaggc aataaaaaac cgccgaattt ggcggttttt
    23641 tattgctggt ctggttcgcg gcctttccag caggttgtat taccgtagta atgcaagcgc
    23701 gtctcagcgg agacaatact cgccagtaac tctctttttg tcaagcaaaa gagagttatt
    23761 attgttctgt tagtgtatta tccactgcgg ccctttccgc cgtctcgcaa gcgggcgctg
    23821 gcttaaggaa aggatgttcc gcgaccgtta gagcaagtgc gtctcagcgc ttgctatcgc
    23881 ggcaatatcg ccagtggtgc tgtcgtgatg cggtcttcgc atggaccgca caatgaagat
    23941 acggtgcttc tgtatcgtac ttattgtttc tggtgcgctg ttaaccgagg taaataataa
    24001 ccggaatctc tccggcgaca atttactggt gattaacaac cttcagagca gcaggtaagc
    24061 ccgaatgccg ccctttgggg cggcatattt tagattatcc gattctgttt aaagtcacgc
    24121 aaaaaaccac cccagcgacg ttcatagaat ggcgcaatat gttcggtaat aaagtggcta
    24181 attccttttt cccctttttt cacctgacaa atatcgatgg gttcatcgcc aggtaatgta
    24241 tcggtcgcta cacttcccgc cacctgaata atttcttcga tatcaccatc ggcttcaatg
    24301 ccaataagta aattaggctg tgcctcttcg ttctctttaa ttgaacaaat aaaagcacgc
    24361 ttcacaggct taatggtttt aaataaggtg gtgagtgaat caatcatttg tgctggcggc
    24421 tctgcgactt ccgataatat cagcgattca ccgccttcca ggatttcctg gctgctcagc
    24481 ggatttccct cttcaccaat caacaaactg atttcacgcg gcataaattc tttaccggtt
    24541 ggcagtttgg cattaaggaa gagcgtttcg ccaagtgtca tctcaaacaa tgtgcgaacg
    24601 ggcattacga caaatgcctg ttcgtcttca accgcctgtt gaagtgcttc taacgaggtg
    24661 aaaaaaggaa tgacgctggt gccgtcttct ttttcccagt gctgtaaatc aagcgcgcta
    24721 tcttcaacca cagcctcgcc ctgcgccgcc gtaccaggca cccagacggt ggattccagt
    24781 agagtacgga aaaaggccgg gcggtgcgcc ggttcggttg ctgctttttc cagcaggtct
    24841 tcaagttcgt tttttgtttc ggacataaga accacaattc attcaacgtt cggggcgcaa
    24901 atgcctgatg cgctacgctt atcaggccta caagggattc gcaatttgtt gaatttgcag
    24961 aatttgtagg ccggataagg cgtttacgcc gcatccggca tattagttta cgccgttaac
    25021 agattagcta tcgtgcgcac accaagtccc gtagcacccg cagaccactg ttcaaccggc
    25081 gctttacggt aagtcgccga gcagtcgata tgcagccagc cttgctgata gttctcaaca
    25141 aagtgcgaca ggaagcccgc cgccgtgctc gcgcctgccg gatacgccgc gcttccggta
    25201 ttgttcagtt cggcaaagtt agacggcagc tggctgcggt ggaattccgc cagcggcaga
    25261 cgccagaatg gttcgttctc ttgtgaggca ctcgccagca aacgaccggc aagcgcatcg
    25321 tcaaaactga acagcgcgtg ataatcatta cccagcgcag ttttcgccgc cccggtgagg
    25381 gtcgccgcat caatgatcat ttccggtttc tgcgcactgg catcaatcag accatcggca
    25441 agcaccagac gcccttcggc atcagtgttc atcacttcaa cttttttacc gttgcgatag
    25501 gtgatgatat cgcccagctt gaacgcattg ccgctaatca ggttatccgc acagcagagg
    25561 aacagcttca cgcgcttgtt cagtccgcgc gtaatggcaa atgccagcgc cccggtaacc
    25621 gttgccgcgc cgcccatgtc cgacttcatc gagtccataa acgcagtctg tttgatgctg
    25681 tagccgccgg agtcaaaagt gataccttta cctaccaggc acgcgtacac tggcgcttct
    25741 ttatcgccag ttgggttgta atccagcgcc agcaataccg gagaacgttc tgaaccgcgt
    25801 ccgacggtgt gcagccccat ataaccttga tcacgcagat cttcaccttt ggtgatccga
    25861 taagtcacac gatcgcccgc gacgttgctg atcagatcaa cagcacgctg tgccagttgc
    25921 gatggtccca attcttctgc cggtgcgttg atggtgtcac gcacccagtc gatgatcatc
    25981 aggcggttat ccagttcctg gcgctgggca tcgtccagat ccggccactc cactttacgc
    26041 gtgcctttcg gggctttgta accttgccag aatgcccagc agcgatccgc atcccagcct
    26101 tcaccgctta actgaacatg cttgatgccc agaccgtcaa tcttgcgcgc cgcacgctgg
    26161 atcagcccca gatcgtctgc cccgttcaaa tgcagggtaa tgccgtcatt attaatgctg
    26221 taagttgctt tttctcccca gcgcgcgtcg gcaggttggg tagagagggt aatcttcatc
    26281 gcttctgtca ttttagttat ccttattcgt ttaacagcaa aacgggctac ccccaggcag
    26341 cccgttcatt cactcggctt cgtccagcca gactaacaaa atcgcttcga ggattttctc
    26401 gttggatgcc tgcgggtcgt cgtcgaaatc ttccagatcg caaatccact gatgcatatc
    26461 ggtgaatcga accgttttcg gatcaagatc gggatacgca tcgtacagtg cttcgccaat
    26521 ttcgcggcta tcggtccact taagtcccat actaacctct gttaatgctc acgcgcatgg
    26581 ttgatagtgt aacgcgggat ttcgaccact aaatcttcgt cggtaacgcg cgcctggcag
    26641 cttaaacggc tttccggctc cagcccccag gctttgtcca gcatgtcgtc ttcctgctct
    26701 gagctttccg gcagtgagtc aaaaccttca cgaacgatgc agtggcaggt ggtgcaagca
    26761 caggattttt cacaggcgtg ttcaatctcg ataccgttac gcagagctgc gtcgagaatg
    26821 gtttcaccgc tattagcttc cagaacagcg ccatcagggc agagatcctg atgaggcaaa
    26881 ataacaatct ttggcatatt aaacctcgtc cacggaatgg cctttcagcg cacgacgaac
    26941 cgactggtcc atgcggcgag cggcgaaatc ctgggtttgt ttgtctacgt ttttaatcgc
    27001 ttgttcgatg gcgtcaacgt cattgccctg cgccacttca ctcaggtgag cggcagcatc
    27061 gtcaatgacc tgacgttctg cggcgcttaa cagcgcggca tcagcagcca gcgcgccgtg
    27121 cagactttcc agcacacgcg ccgcttctac tttttgttct gccagcattc gggcttttac
    27181 gtcctgctcg gcatagctca ttgagtcttt gatcatcgaa gcgatttcgc tatcggtcag
    27241 accgtaagac ggtttgacct gaatagacgc ctcaacgccg gtggatttct ccatcgccgt
    27301 cacgctcaaa agaccgtcgg catcgacctg gaacgtcacg cgaatatgcg caccgccagc
    27361 cggtagcgcc ggaataccac gcagcgcaaa acgcgccagt gagcggcagt cctgcaccag
    27421 ttcgcgctca ccctgcatta catggataga catcgccgtc tgaccatctt taaaggtggt
    27481 gaaatcctga gcgcgggcca ccggaatagt ggtattacgc ggaatcactt tctccaccag
    27541 accgcccatc gtttcgaggc ccagcgacag cgggatcaca tccagcaaca gcatttcgct
    27601 gtccggcttg ttacccacca gaatatccgc ctgaatcgcc gcgccaatag cgacgacttt
    27661 atccgggtcg atggaagtca gcggtggacg accgaaaaat tcgcctaccc gttcacgcac
    27721 cagcggcacg cgagtagaac cgccaaccat caccacttcc agcacttcag aagcctctac
    27781 gccagcgtct ttcagcgcgc gacgacaggc cagtaaggtt cgttttacca gtggcgcgat
    27841 cagttcattg aattgttcac ggctgatttc gccctgccag cccgcaacat tcacggtcac
    27901 agagtccgca tcgctcagcg cgattttggc tgcaatggcg gcatccagca gttcacgctg
    27961 aacgcggtta tcgctacgat caggaatgcc cgcctgctcg cgaatgtaat ccgccagcag
    28021 atggtcgaaa tcatcgccgc cgagcgcgga atcaccgccg gttgccatca cttcaaacac
    28081 gccgcgactt aagcgcagaa tggaaatatc aaacgtcccg ccaccgaggt cataaacggc
    28141 gatcacgcct tcctgaccgg aatccagccc gtaggcgata gccgcagcgg tcggttcgtt
    28201 aagtaagcgc aggacgtgaa ggcccgccag acgcgccgcg tctttggtgc cctgacgctg
    28261 ggcatcgtca aagtacgccg gaacggtgat aactacacca tccagctcgc ctgccagggc
    28321 ttcagttgcc cgcgccgcca gtgctttgag gatgtccgca gaaacgcgca ccgggttcag
    28381 cagccccgcc gccgtttcaa tcatcggcag gccgttttcg ctggcctgga attgataagg
    28441 cagatgcgga tagcgttgct ggatatcagc cagcgagcgc cccatcagac gtttaacaga
    28501 actaatggtg ttggcggtgt caagcgctgc gttagtacgc gcgtcataac ccaccgaatg
    28561 cccttgctgt tgatagtgaa caacagatgg cagcaggtga cggccttcat ggtcggctaa
    28621 cgtttcggcc tgaccgctgc gcactgtcgc caccagcgag ttggttgtgc ccaggtcaat
    28681 accggccgcc agacgacgct gatgcggcgc agcactcaaa ccaggttcac taatttgtaa
    28741 taaggccatg tttagcttcc agaaattaaa aatcgagcag tttttcttcg agttgttcgg
    28801 cactgcttcg cagtttatcg agaaaacgca gcttacgcac ggtatccgcc gccgcggccc
    28861 acgtctcgtt gtctaactgt tcaaccatca actgatggcg ggtatcaaac atctttttca
    28921 cacgtttgat aaagctttcc agccgcgctt catctttcgc ctgttcgatc tcgtccagct
    28981 cttcgcgcag ctccagctgt tccataagaa acgcggtgtc gcgcacagta tgctgctcgc
    29041 tggcgagatc aaaaccgtgc aaagaaagca aatattccgc gcgcattaac ggatgacgca
    29101 gcgtttgcca tgcctggtta atggttgcag attgctgtac ggcggcgagt tgttccgcct
    29161 ggcttccgct ggcgaattta tcaggatgat actgacgttg tagatcctga aaacgcaggc
    29221 tcagtgcctg ggtatcgagt tgataacggg caggcaagcc aaagagggtg aagtaatcca
    29281 taacattctc agggctgcga tccgccgcga taacggcgaa tcaggtaaaa caaaccccac
    29341 gcgcaggcga ccacggtggg gttatcggta tgcgcatcaa acgtggaagc tttcgccgca
    29401 accacactca tctttaacgt tcgggttggt gaatttaaac ccttcgttca ggccttcttt
    29461 tacgaagtcc agctgcgtac cgtccagaaa ttgcaggctt ttgccatcga ccacgacttt
    29521 cacgcctttg tcttcaaaca cgatgtcttc cggcgtcggt tcgtcaacaa attccagtac
    29581 ataagccata cctgaacatc cggaggttct cacgcccaga cgcaggccaa accctttgcc
    29641 gcggttagcc agaaaggtat tgactcgcgc tgctgcactg tcgctcagtg taatcgacat
    29701 aaccaaacct caactcttat tttgcttcac gtttgctttt atagtccgca atggcggctt
    29761 tgatcgcgtc ttctgccaga atagaacagt gaattttcac cggcggcagt tcaagttctt
    29821 cagcaatatc ggtgtttttg atcgcctgcg cttcgtcgag agacttccct ttcacccatt
    29881 cggtgaccag ggagctggaa gcgatagcag aaccgcagcc gtaagtttta aaacgcgcgt
    29941 cttcaatgat accttcatcg ttgactttaa tctgcaactt catcacgtcg ccacaggccg
    30001 gcgcacccac catgccgctg ccgacgttct cgtcgttgtt gtcgaaggaa cccacgttac
    30061 gcggattctc gtaatgatcg ataacttttt cgctgtaagc cattataaat tctcctgatt
    30121 ccgataccga ttaatgatga gcccattcga tgctgttcag atccacgccc tgcttgtaca
    30181 tttcccacag cggagaaagg tcacgcaaac gaccgatgga tttacgaact aactcgatgg
    30241 tgtagtcgat ctcttcttca gtagtaaaac gacctaaaga gaaacggata gagctatgtg
    30301 ccagctcgtc gttcagcccc agcgcgcgca gcacgtagga cggttcgagg cttgctgacg
    30361 tacaggcgga acctgaagaa accgcgaggt ctttcagcgc cataatcagc gactcacctt
    30421 caacgtagtt aaagctgacg ttgagaatgt tcggcgcacc gtgttcgagg tcaccgttca
    30481 ggtaaacttc ttcgatatct ttgatgccgt tccatagacg gttacgcagg ccgcgcagac
    30541 gttccatctc ggtcgccatc tcttcttttg cgatgcgata ggcttcgccc atgccgacga
    30601 tctggtgaac aggcagagtg ccggaacgca taccgcgttc gtgaccgccg ccgtgcattt
    30661 gcgcttcgat gcgtacgcgc ggtttacgac gcacatacag cgcaccgata cctttcgggc
    30721 catagatttt gtgaccggag aaagacatca ggtcaacttt caactggctc aggtcgatag
    30781 gcagtttacc cacgctctgg gttgcatcaa cgtgatagat aatgccacga gcacggcaca
    30841 tttcgccgat agccgcgata tcctgcacca cgccgatttc gttatttacg tgcatgatgg
    30901 acacgaggat ggtgtcgtca cgcatcgctg cttcaagttc tttcaggtcg ataatgccgt
    30961 tacgctgcgg tgccaggtag gtgacttcaa aaccttcgcg ctccagctga cggcaggtat
    31021 ccagtaccgc tttgtgttcg gttttgctgg tgatgatgtg cttgcctttt ttctgataaa
    31081 agttggctgc acctttgatc gccaggttgt cagattcggt tgcaccagag gtaaagacga
    31141 tttcacgcgg atcagcgccg accagatcgg caatctgatt acgggcgata tctaccgctt
    31201 cttcagcctg ccagccgaaa cggtgagaac gggaggccgg gttaccaaag gttccgtcca
    31261 tcgtcataaa ctgcatcatt ttctcggcaa cacgcgggtc caccggcgtg gttgcggagt
    31321 agtcgagata aatcggtaat ttcattgctc tataaactcc gtacatcact caatgcaagg
    31381 aatcaggcta ccggctggat gtacgaccgt gtttacggag tatttagcac tccggcctga
    31441 ttctgaattc tttttattaa gcgcgtaact taacgtcgat cgcgtcttgt gtgcgggtgc
    31501 gtggcgcgtc gtgagtatgc tgacgaccag acacatccag cacttcctgg ttattaacca
    31561 gttcgcctaa agtaatgttg ttgagaaaac cggtgagacg gtcgctcaaa tcacgccaca
    31621 gcgcgtgggt caggcattta tcgccgccct ggcagccgcc tttaccctga caacgggtgg
    31681 catctacaga ttcgtcaacg gcgctaatta cttcgccaac ggcgatgctg ctggcatctt
    31741 tgcctaacag ataaccaccg cctggtccac gtacgctgga aaccagacca tttttacgca
    31801 gacgggaaaa cagttgttcc agataagaaa gggaaattcc ctgacgttcg gaaatatcag
    31861 ccaacggtac cgggcccgct tcagagttga gcgcaacgtc aagcattgcg gtcacggcat
    31921 agcgcccttt agatgtcagt ctcatgtctt acttcacctc aaactcgccc ctgcccgggg
    31981 ttttttattg taaaagtggg ggtattgcat agcagggtca agtctgacat tcccgagtaa
    32041 attggtcaac tatttacttg actgatttag tcgggtattt aaccttcagt gccatttttt
    32101 tatcgtggcg tttgtacctg ttgtcggatg cggtgtgaac accttatccg acctacggtt
    32161 ctgtacattg taggcctgat aagacgcgtt aagcgtcgca tcaggcaacc actgttattc
    32221 ggccttgtta cctttattct gctgctcaat agaagccaga atcccgcgca ggatattcaa
    32281 ttcctggctt tccgggcgcg cacgggtaaa cagacggcgc aatttattca tcacctgccc
    32341 cggatggttt tcacggataa aaccggttgc cagcagcgtt tgttccagat gaccataaaa
    32401 acgctccaga tcatcgacca gcggatacgg cgtctcttca tgctcgacct gctcgccatt
    32461 ttcctgagtt gccagccagg ccatgcgcac ttcataggca ataacctgaa ccgccatcgc
    32521 caggttcagc gagctgtatt ccgggttagc cgcaatcgcg acatgataat ggcatttctg
    32581 caactcttca ttggtcaggc cgacacgttc gcgaccaaaa accagcgcca ccggggtatt
    32641 tgccgcttca gcaacgcttt tcaaaccgca ttcgcgcggg tcgagcatcg gccacggcag
    32701 cgtgcgggaa cgtgcgctgg tgcccaccac caggctacaa ccggctaacg cttcgtcgag
    32761 cgtatcgacg atgtgagcat taccaatcac atcgctggcc cccgccgcca gggcaatcgc
    32821 ctgggagtca ggtttcacca gtggattaac cagccacaga ttggttaatc ccattgtttt
    32881 catggcacgg gcaacagaac ccatattgcc ggtgtgtgac gtctccacca gcacaattcg
    32941 aatattttgc agcattgtct ttcttcgtct aaagattatt cgcgcatctt atcataaaac
    33001 gaagacagat gccgatctcg ctgctatact ctgcgccgtt ttcccgttct ttaacatcca
    33061 gtgagagaga ccgatgcatc cgatgctgaa catcgccgtg cgcgcagcgc gcaaggcggg
    33121 taatttaatt gccaaaaact atgaaacccc ggacgctgta gaagcgagcc agaaaggcag
    33181 taacgatttc gtgaccaacg tagataaagc tgccgaagcg gtgattatcg acacgattcg
    33241 taaatcttac ccacagcaca ccatcatcac cgaagaaagc ggtgaacttg aaggtactga
    33301 tcaggatgtt caatgggtta ttgatccact ggatggcact accaacttta tcaaacgtct
    33361 gccgcacttc gcggtatcta tcgccgttcg tatcaaaggc cgcaccgaag ttgctgtggt
    33421 atacgatcct atgcgtaacg aactgttcac cgccactcgc ggtcagggcg cacagctgaa
    33481 cggctaccgt ctgcgcggca gcaccgctcg cgatctcgac ggtactattc tggcgaccgg
    33541 cttcccgttc aaagcaaaac agtacgccac tacctacatc aacatcgtcg gcaaactgtt
    33601 caacgaatgt gcagacttcc gtcgtaccgg ttctgcggcg ctggatctgg cttacgtcgc
    33661 tgcgggtcgc gttgacggtt tctttgaaat cggtctgcgc ccgtgggact tcgccgcagg
    33721 cgagctgctg gttcgtgaag cgggcggcat cgtcagcgac ttcaccggtg gtcataacta
    33781 catgctgacc ggtaacatcg ttgctggtaa cccgcgcgtt gttaaagcca tgctggcgaa
    33841 catgcgtgac gagttaagcg acgctctgaa gcgttaatga ctcaggcggg tgatatcaat
    33901 cacccgcccc cgcctttcag gcgctattcc gaaatacttc ctcaccgctt tactttcttt
    33961 cgtcactctc ccaccatctt tccccgatta atggatgata aagaagtcgc aaggaaaggt
    34021 tatggcactg ccagtgaaca aacgcgttcc caaaattctg tttattctct ttgttgttgc
    34081 cttctgcgtt tatttagtgc cgcgcgttgc catcaacttc ttctattatc ccgacgataa
    34141 aatttacggt cccgatccct ggtcggcgga atccgtcgaa tttacggcta aggacggtac
    34201 tcgtctgcaa ggctggttta tcccttcttc gacgggccct gctgacaacg ccatcgcaac
    34261 catcattcat gctcacggca atgccggaaa tatgtccgcc cactggccgc tggtcagttg
    34321 gttacccgag cgtaatttca acgtttttat gtttgattat cgcgggtttg gtaaatcaaa
    34381 aggcacgccg tcccaggccg gattgctgga cgatacgcaa agtgccatca atgtggtgcg
    34441 ccatcgcagt gatgtaaacc cacaacgtct ggtgctgttc gggcagagta ttggcggggc
    34501 gaatattctg gatgttattg gtcggggtga tcgtgaaggc atacgtgcgg tgatcctcga
    34561 ctccacattt gcctcttatg caaccatcgc caaccagatg atccccggca gtggctactt
    34621 acttgatgag agttacagcg gcgaaaatta tatcgccagc gtcagcccga tcccgctttt
    34681 actcattcac ggtaaagctg atcacgttat cccatggcag cacagcgaaa agttgtatag
    34741 cctggcaaaa gagccaaaac ggttgatcct aatcccggat ggcgaacaca ttgatgcttt
    34801 ttccgatcgt cacggcgatg tttatcgcga acagatggtg gactttatcc ttagcgcgtt
    34861 gaatccacag aactaaccca tgatcgctag cacgataatc attcacaaaa ccaccttaag
    34921 acatgctaat ccactggtca gaacagttta agatgagaaa aattctgtga cgcttgccaa
    34981 catttctgat gattagcatt cccttcgcca tttccctgag caaactttag ctattcttat
    35041 caattatgct tatgggagat ctacagatga tgcctacgct tgctccacca tctgtccttt
    35101 cggctcccca gcgccgctgc cagatcttgc tgacgctctt tcagccgggg ttgaccgcca
    35161 ccacggcaac tttcagcgag cttaatggtg tggatgatga tattgccagt cttgatatca
    35221 gcgaaacagg acaggagatc ctgcgctatc atcaactcac actgacggct ggttatgacg
    35281 gtagctaccg ggttgaaggt acagtgctta accaacgttt gtgtttattt cactggctac
    35341 gacgtggttt ccgtctgtgt ccatcattta ttaccagcca tttcaccccc gccctgaaga
    35401 gtgaactgaa gcggcgcgga attgcgcgta acttttacga cgataccaat ctacaagcgt
    35461 tagtgaatct ctgctcccga cgactgcaaa aacgctttga aacgcgcgat attcatttcc
    35521 tgtgtctgta tctgcaatat tgtttgctgc aacaccacgc tggaattacg cctcagttta
    35581 atccgctcca acgtcgctgg gcggaatcct gccttgaatt tcaggtagcg caggaaattg
    35641 gacgccactg gcagcgtcgg gcgctccagc ctgtaccgcc tgatgagcca ctgtttatgg
    35701 cattactttt ttccatgttg cgggttcccg atccattgcg tgatgcgcat cagcgagaca
    35761 gacaattgcg tcagtctatc aaacgtctgg taaaccattt tcgtgagctg ggaaatgttc
    35821 gtttttatga tgaacagggg ttatgcgatc agctttatac ccacctcgcc caggcgttaa
    35881 atcgcagttt gtttgccatc ggtattgata ataccctgcc ggaagagttc gccagactgt
    35941 acccacgcct ggtgcgcacc acccgcgcgg cgctggccgg atttgaaagt gaatacggcg
    36001 tccatctttc tgatgaggaa agtggtctgg tcgcggtgat tttcggtgcc tggctaatgc
    36061 aggaaaacga tctgcatgaa aaacagatta ttctactgac cgggaatgat agcgagcgag
    36121 aagcgcagat tgagcagcag ttacgcgaac taacgttact gccgctcaac attaagcata
    36181 tgtcggtaaa ggcatttttg cagacaggcg ctccgcgtgg cgcggcactg attattgcgc
    36241 cttataccat gccattaccg ctcttttcac caccgctgat ctatacggac ctgacgctga
    36301 caacacatca acaggagcag atccgcaaaa tgctcgaatc agcatgaggg aacaactttc
    36361 gggcgcaaaa acattgccgg cagcgccacc agcgccatta cccagaacac gccgtggccc
    36421 agatattgat acaggaaacc ggcgaaaacg gtcatgatag cgatactgcc gcccatcgcg
    36481 acggcagagt aaaccgcctg taaacggatg acttcgctac cctggcgagc agcaatataa
    36541 cgcatggcgg ccaggtggca gaccgtgaag gtgccgcaat gcagaatttg caccactatc
    36601 aaccacggca acgccgtagt tgctcccata atgccccagc gcactacgcc gcaaatcgcc
    36661 gagatcaaca gcatatcgcg tgcactacaa cggcggaaaa gtttattact cagcgcaaag
    36721 ataatgactt ccgccaccac gcccagcgac cacaaatacc ccaccgccga ggccgagtag
    36781 ccagctgcct gccagtaaat ggcgctaaaa ccgtaatagg ccgcatgtgc cccctgcaat
    36841 aaacaaacgc aggccagaaa gcgccagttc tggcgaacca gcgccaacca cgctgaccaa
    36901 ccggtgctct cctgctggcg gcttgcccct tgtggctgaa tcgtcggacg gatgagaaag
    36961 ccgagcagca tggatgccac gcccaacgtc aacagcgcga ggatcacccg ataatcaaac
    37021 atagtgacca gtttgcccgt cagcgccgag ccaatgacaa acgccaccga gccccacagt
    37081 cgcactttgc cgtaatcaag cgggaactgc ttttgccacg tattcgccag tgcatcggtc
    37141 aacggtacca gcggtgagaa aaagaggtta aagccaatca tcaccagcat cagccacgct
    37201 acgtgcgccc ccgcccagaa ggcgacagca aagagaagtg tcagcagtgc cagcacgcgc
    37261 aaggcggaaa tcaggcggga aggatcgctg acgcggggcg cgatgagcaa actcccgaga
    37321 aaacgggcaa ccagacctgc ccccaataac aggccgatgg tttctggcgt taaaccaatc
    37381 cctttaagcc agacgctcca gaaaggtaga aaaatgccgt aactaaaaaa gtatgtgaaa
    37441 tagccgagcg ccaaccagcg cgtggattgc aaaaccatga gtccctcccg tcaggaggcg
    37501 ttagtctggg gttaatcgaa tgttggtgca agttgaaaaa taagaatatt cgagcctgct
    37561 tcccgatgtc tgtaacgcca gttcacaagc actaaaaaac tgagatccgc gacgcatgtt
    37621 gcaaaaataa tgcgtaatta tgccgttacg cttgccaaac gttcctgcac aatggcgatg
    37681 aaatcgcgta atgccggctt catctcccct ttcttccacg ccatcagtaa agcaatggaa
    37741 ggcacatttc cggcaatggg acgaaaaaca acctgtccgg tattaaaatt attcatataa
    37801 ccgggtatca aagtgacgcc cagccccatg cccaccagat tcatggtcac cagaatattc
    37861 gttgccacct ggacgatatt tggctggctg ttttcttgcg caaaccacgc tttaacgatc
    37921 ggcgcaagcg aaccggaata cgccggatcg gtactgacga aattcacgcc atccagttgc
    37981 gcggcggtga tctctttttc atgtgctaaa gggtgatcaa ccggtaacac aaccaccaat
    38041 ggttcgtcaa aaagctccag ataatcaatc tccgggctat aaacaggatg gcgcattaag
    38101 ccgacgtcga gttcaccacg gcgaattttt tcctcctgtt gcgtggtgat taaactcacc
    38161 agctcaatca aggtgtctgg ctgtctgaga cgaaacatcg gtaatacttt tggcagtaaa
    38221 ttcacttccg ccgatggcac aaagccaatg gttaattgtc tgtcttcctg aacaattttc
    38281 cgcgcccgta atttggcatt ttccgcttgt tcgaggattg ccagcgcgtc ctggagaaaa
    38341 cattctcccg ccgctgtcaa cgcgactttg cgcttatccc tcaccagtaa cggaacaccg
    38401 acacagtttt caagatcgcg gatctggctg cttaacgaag gctgtgaggt atgcagtttt
    38461 tccgccgcac gggtaaagtt cagtgcctgc gccactgcga cgaaatagcg taaatgccgt
    38521 agttccatca ccttcccctt gttatcgaaa aaacgtctca gctggtagaa attaactatt
    38581 tcacatatta gcaaccaacc agcaacatcc ttatggcaca aaaatagaag gtcaatacat
    38641 cttatctttc aggattaaaa aatatgacca caccctcaga tttgaacatt taccaactga
    38701 ttgacaccca aaatggtcgg gtcactccgc gtatttatac cgacccggat atttaccaac
    38761 tggagcttga gcgtattttc ggtcgttgct ggctatttct cgcccacgaa agccagatcc
    38821 caaaacccgg tgatttcttt aacacctaca tgggagaaga tgcggttgtc gtagtgcgtc
    38881 agaaagacgg cagcatcaag gcgtttctca accaatgccg ccaccgggcc atgcgtgtga
    38941 gttatgcaga ttgcggcaac actcgcgcct ttacctgccc gtatcacggc tggtcttatg
    39001 gcattaacgg cgagttgatc gatgtaccgc tggaacctcg cgcctaccca caagggttgt
    39061 gtaaatccca ctggggacta aacgaagttc cttgtgtgga gagttataaa gggctgattt
    39121 ttggcaactg ggataccagc gcaccgggcc tgcgtgatta cctgggtgac attgcctggt
    39181 atctggatgg catgctggat cgtcgcgaag gcggcaccga aattgtcggc ggcgtacaaa
    39241 agtgggtgat caactgtaac tggaaattcc cggcagagca gttcgccagt gaccagtatc
    39301 atgctctgtt cagccatgct tctgccgttc aggtattagg ggcgaaagat gatggcagcg
    39361 ataagcgcct cggtgatgga caaaccgccc gcccggtgtg ggaaaccgcc aaagatgcgc
    39421 tgcaatttgg tcaggacggt cacggtagcg gtttcttctt tactgaaaaa ccggatgcta
    39481 atgtctgggt cgatggcgca gtttcaagct attaccgcga aacctatgcc gaagcagaac
    39541 aacgtttagg tgaagttcgc gccctgcgcc tggcgggtca taacaatata ttccccacgc
    39601 tttcatggct aaacggcact gccacactcc gcgtctggca tccgcgcggc cctgatcaag
    39661 ttgaagtgtg ggcgttctgt attactgaca aagccgcctc cgatgaagtt aaagccgctt
    39721 ttgaaaacag cgccactcgt gcttttggtc ctgctggttt tctcgagcag gatgactcgg
    39781 agaactggtg tgaaatccag aaattgctga aaggtcaccg cgcccgcaac agcaaactgt
    39841 gtctggaaat ggggcttggt caggaaaagc gtcgcgacga cggcattcca ggcattacta
    39901 actatatctt ctcagaaacg gccgctcgtg gaatgtacca acgctgggcc gatcttctga
    39961 gtagcgaaag ctggcaggaa gtgctcgata aaaccgccgc ttaccagcag gaggtgatga
    40021 aatgagtgcg caagtttcac tagagttaca tcaccgcatt agccagtttc tctttcacga
    40081 agccagctta ctggacgact ggaaatttcg tgactggctg gcgcagctcg acgaagagat
    40141 tcgttacacc atgcgcacca cagttaacgc gcaaacacgc gaccgccgca aaggcgtcca
    40201 gccaccgaca acctggattt ttaatgacac caaagaccag ctggagcggc gaatcgcccg
    40261 tctggaaacg ggcatggctt gggcagaaga gccgccgtca cgcacccgcc acttaatcag
    40321 caactgccag ataagcgaaa ccgacatccc aaacgtattt gctgttcggg tgaattatct
    40381 gctttatcgg gcacaaaaag agcgcgatga aacgttctat gtcgggactc gttttgacaa
    40441 agttcgccgt ctggaagatg acaactggcg cttgctggaa cgggatatcg tcctggatca
    40501 agcggtaatc acttcccata acctgagtgt actgttctga tgaatcgaat ttatgcgtgt
    40561 cccgttgcgg atgtgccgga gggtgaggct ctccggatcg atacctcgcc cgtcatcgcc
    40621 ctgttcaacg ttggcggcga gttttatgcc attaacgatc gttgcagcca tggtaatgcg
    40681 tcaatgtcag aagggtatct ggaagatgac gccacggtgg agtgcccgct acacgccgcc
    40741 agtttttgcc tgaaaacggg gaaagcgtta tgcctgcccg ccaccgatcc gctcaccact
    40801 tatccagtac acgttgaagg tggtgacatt ttcatcgact taccggaggc gcagccatga
    40861 gcgatctgca taacgagtcc atttttatta ccggcggcgg atcgggatta gggctggcgc
    40921 tggtcgagcg atttatcgaa gaaggcgcgc aggttgccac gctggaactg tcggcggcaa
    40981 aagtcgccag tctgcgtcag cgatttggcg aacatattct ggcggtggaa ggtaacgtga
    41041 cctgttatgc cgattatcaa cgcgcggtcg atcagatcct gactcgttcc ggcaagctgg
    41101 attgttttat cggcaatgca ggcatctggg atcacaatgc ctcactggtt aatactcccg
    41161 cagagacgct cgaaaccggc ttccacgagc tgtttaacgt caatgttctc ggttacctgc
    41221 tgggcgcaaa agcctgcgct ccggcgttaa tcgccagtga aggcagcatg attttcacac
    41281 tgtcaaatgc cgcctggtat cctggcggcg gtggcccgct gtacaccgcc agtaaacatg
    41341 ccgcaaccgg acttattcgc caactggctt atgaactggc accgaaagtg cgggtgaatg
    41401 gcgtcggccc gtgtggtatg gccagcgacc tgcgcggccc acaggcgctc gggcaaagtg
    41461 aaacctcgat aatgcagtct ctgacgccgg agaaaattgc cgccatttta ccgctgcaat
    41521 ttttcccgca accggcggat tttacgggtc cgtatgcgat gttggcatcg cggcgcaata
    41581 atcgcgcatt aagcggtgtg atgatcaacg ctgatgcggg tttagcgatt cgcggcattc
    41641 gccacgtagc ggctgggctg gatctttaag gaagcacgat gaaagaaaaa accatcatta
    41701 ttgtcggtgg cgggcaagcg gcggcaatgg ctgcggcctc gctacgccag caagggttca
    41761 ccggtgagct gcatctgttt tccgatgagc gacatcttcc ttatgaacga cctccactct
    41821 cgaaatccat gttgctggaa gattccccgc agttacagca ggtgttaccc gctaactggt
    41881 ggcaggaaaa caatgttcat ctgcattccg gtgtaaccat caaaacgctg ggtcgcgaca
    41941 cacgagagtt agtgttaacc aacggcgaaa gctggcactg ggatcagctt tttatagcaa
    42001 ccggcgcggc agctcgaccg ctgccgttgc ttgatgcact gggagaacgc tgctttaccc
    42061 tacgccatgc cggtgatgcc gccagactgc gagaagttct gcagcccgaa cggtcagtcg
    42121 tgattgtcgg tgccggaact attggtctgg aactggctgc cagcgccacg cagcgcagat
    42181 gtaaggtgac agtgattgaa ctggcggcaa ccgtcatggg ccgtaatgca ccaccgcccg
    42241 tgcaacgcta tcttttacag cgccaccagc aggctggtgt gcgcattctg ctcaataatg
    42301 ccattgaaca tgtggtcgat ggtgaaaaag tagaactgac gctgcaaagt ggggaaacgc
    42361 ttcaggctga tgtggtgatt tacggtattg gtatcagcgc caacgagcaa ctggctcgcg
    42421 aggccaacct tgatactgcc aatggcattg tcattgatga ggcttgccgc acctgcgatc
    42481 ccgcgatctt tgccggtggc gatgtggcaa tcactcgtct tgataatggt gcactacacc
    42541 gctgcgaaag ctgggaaaac gccaataacc aggcgcaaat tgccgctgcc gcaatgttag
    42601 ggctaccgct accgctactg ccgccgccgt ggttctggag cgatcagtac agtgataact
    42661 tacagtttat tggcgatatg cgtggcgatg actggctttg tcgtggcaac ccggaaactc
    42721 agaaggcgat ttggtttaat ctgcaaaacg gcgtgcttat cggtgcggtc acgctgaatc
    42781 aggggcgtga gattcgccca attcgcaaat ggatccagag cggcaaaacg tttgatgcga
    42841 aactgctgat agatgagaac atcgcgctta aatcactgta accaggataa ttagcgaata
    42901 tctcaatgcc tggggcgtgg cgaggtgcaa gagtgtgtat tacgtttaaa tcacattatc
    42961 ttgcaaaggg attggttatg aacacactac gttattttga ttttggagct gcccgccccg
    43021 ttttgttatt aattgcccgt atcgccgtgg tcttaatttt cattattttt ggttttccca
    43081 aaatgatggg ctttgacggt acggtccaat atatggcctc gttgggcgcg ccgatgccga
    43141 tgctggcagc gattattgcg gtagttatgg aagtgcccgc cgcgatatta atcgtgcttg
    43201 gctttttcac ccgtccgctg gcggtgctgt ttattttcta cacgctgggt acggcggtga
    43261 ttggtcacca ttactgggat atgaccggcg atgcggttgg gccaaatatg attaatttct
    43321 ggaagaatgt cagtatcgct ggcgcattcc tgctgctggc aattaccggg ccgggggcaa
    43381 tttctctcga tcggcgttag gaaagatgcc ggatgcggcg tgaacgcctt atccggcatt
    43441 taataaatta cagccactct acccgcaacg acatctctga agttgttgat tcccctggtg
    43501 ctagcgcaat gagatcaccc ccttccgggc gatgatgatc atccggcgca tggctcatcg
    43561 gttccagaca gaagaaatca aacgcatatc ctttatcaaa cgcagggtcg gaaacaaaga
    43621 tgaaataaca cggtgcaggt ggcgtcgttt ccatgatgat ggcataacct tcctgcggct
    43681 gctcgatgcg ggcctgacca ttccatccgg caaaaccatt gttcacccac tggcgcggca
    43741 acggcgcggg ctggttaaaa tccagttcct gcggtagctg ctcgcaaaac tcacccgcca
    43801 gccactgctc ccgctccagc cagtaaccgc tcgcctgtgc ctgaatccgc gtttgcggcg
    43861 acaacggaaa ataaggatgc cagccggtac caaatggcag cgtctctgcc ccttgattgg
    43921 tgacagagag cgtcaccgtc agcgtatccg ccgttaaatg aaacgcctga cttacccgat
    43981 agtgatagac accgctgcga tgttcataca ccagacacaa actatcatcg ctgtgcgaga
    44041 cacattgcca ctcgcccagc cagccatcgc cgtgtagata gtgcgcatcc cactcaacat
    44101 tcggttgcag ttgatactca cgcccctgcc agacaaaacg attgccgctc acccggtttg
    44161 caaatggcac cagcggaaaa catgaggcat cggttgccac accgcttttt ttaccaggac
    44221 gtaagagcgg cgtcgtatcg cgccagaagc cttcgattac gccgccctgg tcagaaacgt
    44281 ccagctttag cgacccgtgg gataaggtat agatggtcat ccgctaactc cttaatccgg
    44341 gaagttaatc acaactttcc cgcatttgcc gctcgccatc agcgcatagg catcacctgc
    44401 ctgttccagc gagaagcgat gggtaatggc gttacgcggc cacagcttcc agtccgtcag
    44461 atcatgggcg catttttcca tatggaacag actggtcacc caggagccga taatccgccg
    44521 ttgatggtgc atcagatcgg cgctgacctc gaattccact tttccggttt caccaatgta
    44581 aaccacccgt ccccagtcag cggtggattg cagtgccagc aagcgacctg cggcattacc
    44641 ggaacaatcg agcgcaacat ccgcgccacc gtgggtgagt tcggcgataa tctgcggcag
    44701 accttcggtg gttgctaaat agccgtgatc catcacccct aactgttttg ccatcgccag
    44761 acgttccggc agcatatcaa cgccgatgat ccgttttgca ccgcgacctt tcgccagcat
    44821 catcgccatc atgccgaccg gccccagacc gaccaccagc acgttatcgc tgccggaaac
    44881 ttcgccgcgc aaaatccctt cataggccgt gccaacgccg caactgataa acgcaccatc
    44941 ttcgtagctc agcgcatccg gcaggaggat cagatctttt tcttccgcca gcagatattc
    45001 ggcatgaccg ccgtcacgct gccagccgta agccgctttt cctttgccag tacaagagat
    45061 aggaaagccg cggcggcagt tcgggcaaaa accacagcca gaaatgtgat acaccagcac
    45121 gcggtcgccc tctttaaaat ggcggcagcc ttgccccatc gccacaatct gcccgcacgg
    45181 ttcatgaccg ttgataaatc cctggtataa cggtttgtcg ggtgccgctg ccgtagcgcg
    45241 gtgctggtgg tagatatagt ggacatcgct tccgcaaatt ccggaagatt tcattttgat
    45301 cagtacctgg ttaatccctg gtgtcggcac cgcaacttcc cgtagatcga cggtcgaatt
    45361 tcctggtaaa taagctgcca gcatcgtttt cataaatccc tcattaacaa tacgattaat
    45421 tttcatccct gccgcacccg cgccaggggc gttaaggttt agcgtttcgc tttactgctt
    45481 cgctgggtca gcaagatatt cgccagcacc gccaccacga tgatgacgcc gcgtaccacc
    45541 tgctggaaaa aggagttaat accgagcagc accagaccgt taccgattag cgtaatcacc
    45601 agcacaccaa gcaatgtacc gaacagggaa ccgcgaccgc cggaaagtgc cgtaccgccg
    45661 accacgaccg cggcgatgac gtcaaactcc agaccgtttg cggcacctgc gttaccagaa
    45721 ccgaggcgcg ccgccaacaa aatgccggtc accgccgcta ataatcccga aagggtaaag
    45781 ataagaatgc gcacccgacg aacgttgatg ccgcacaact gcgccgccgt agcattaccg
    45841 ccaacggcaa aaaccgagcg cccgaaggcg gttttgcggc tgatgaacac aaacagcgca
    45901 aacaacacaa tcatgatcag cgcggatacc ggcacaccga gaaattgtcc gcccagccag
    45961 tccagcacct cgttttcgtc aatcggcact ggcagtgcgt tcgtcataaa cagccccatt
    46021 ccgcgcaggg cgctccacag ccccagtgtg gcaacgaaac ttggtacgtt aaacacgccg
    46081 cgcagcaccc cggcgagcgt tcccatcagc gcgcctaaca gcaacaccag cagacaagca
    46141 atcgccaacg gaacttcaaa ttgcagcaaa aatgccaggc acaccgagac aaaagccacc
    46201 atcggcccaa cgctgacatc aatctcaccg gagataataa tcagcgtcat cgcccaggcg
    46261 gcaatcccaa tggtggcggc atcgcgcagc acgttcatct ggttattcaa tgagataaag
    46321 ccaggcgcgt tcagggagaa gaccagataa agaatggcaa tcaccaccag caaaccgatc
    46381 tcattaatat ggcgactgac aaattgtttg agcgagacgc tcttgccctg cggcaatggt
    46441 aatgacgaag cagacatctt caggttcctc ggtaaaattc atcagtgcac agacagaatg
    46501 gcggacatca gctcatccac attgaccggc gagtgaaact cctgcgagaa cgtaccgtgc
    46561 tgtaacaaca ggatgcggtc gcacaccagc ggcaactcct ccacttcact ggagataaac
    46621 accacgcttt ttccttcggc agccagctca cggacaatac ggtaaatctg ctgtttggct
    46681 tcgatatcga cgccgcgcgt tggctcgtcg agcaacaaaa tctggctggc ggcatagacc
    46741 caacgaccga tcaccacttt ttgctgattg ccgccagaaa gcgtgccgat gggcgtttcg
    46801 ctactggcgg ccttgaccgt catccgctgc atcacctctt cggtcaggcg gcggatggtg
    46861 gaccattgca gtacgccgtt ggcgctgatt ttttgccgat tggtcagcac tgtattttcg
    46921 tcaacgccca accagggaat gatccccgct tctttgcggt tttctggcgt atagccaatg
    46981 ccgcgtttca gcatgtcgcc gtaatcgggg cgcgtgattt tctcgccgtt gataacaatt
    47041 tcgccctgtt catactcctc cagcccaaca atcgccttca gcaattcact gcgccctgcc
    47101 cccagcaggc cagcaatgcc aagcacttcg ccgcgacgta acgtaaagct gatatcctcc
    47161 agcttgggct tatggcgtaa tgcacggact tccagcacgg cctgatcgac aatttcctga
    47221 ggggctaccg gcgcaatatc aacgtgatcg cgccctagca ttagcgacac aatatgatgc
    47281 gtggaggtgt tttcgagcat cacatcgccc gccacctgac catcgcgcat aacggtggca
    47341 caggaggcaa tgcggcgaat ttcttccatc cggtggctga cataaatcac cgccacgccc
    47401 agtgccgaca tctttttcac cgcgctgatc accagttcaa cttccgcact ggcaagcgaa
    47461 ctcgtaggtt catcaagaat gaccacgcgc ggctcgccct tcatcacccg tgcaatttcc
    47521 accagttgct tttgcgccgg gcttagcgtt gaaacaagtt gttcaggact aacgtcaacg
    47581 cccagcgcct gtaagcaacg ttgggcatcc tgcgccattt gcaggtaatc aatcatgccg
    47641 ttgcggcggg gccactgacc gaggcagagg ttttccgcca ccgtcagccc ttccaccaga
    47701 cttaactcct gataaaccgc acgaaccccc agttcagcgg cacggcgagt caacgtagct
    47761 tcgtcacctt ccagtcgcgt ctcgccaatc cagatatcac cgctatccgg gcgttcactg
    47821 ccggtaagca ttcgaatgag agtcgatttg cccgcgccgt ttttacctaa cagcgcacga
    47881 acttcgcctt tattgagcgt gaagttaacg ttatccagcg caacgacgcc ggggtaacgc
    47941 ttatttcctg ccaccacttt tgctaccggg actgcctctg ttgccgtgaa catagggacc
    48001 tctgcgaatc agcgattagg gcagaccatc aacgtgcgtt gccagccact gtttaccatc
    48061 ttccgttttg gtatagagat cgattggcac ctgaatcact ttttcaccgt cggcttgttt
    48121 attgataacc ttcaatgttt gcgcgagaac agcattgccc attttcttac cggaaatatc
    48181 cactaccgct ttcagcacct gattgttttc cagctcctga gcaatttcag tggtcatatc
    48241 cgaaccgaaa acagcaattt ttccggcctg attttgatta cgtaccgctt ttaccgcgcc
    48301 gagtgtcgct ccgcccgatt cacccataat ggcgttgaga tccggcgtgg agataattag
    48361 tttttcacca acggaaatcg ctttatctaa aacagtccct tcctgattag cgacaatttg
    48421 cgcgccggga acgcgggatt ttaatacttc ttcaaatcct ttacgtcgct gcacacaaac
    48481 ttcaaaggct tcgcaattga tgacggcaat tttcggctgg tcaattttat tggcaataaa
    48541 ataatcggcg gcagcattac ccagtttttt accaaattcc agcggatcgc cgaccagata
    48601 cgccgagaca tatttatcga cacccttttg attaatacag gtgttgtagc aaatcaccgg
    48661 aatacccgct tcactggcgc gacgaacggt acggctactg ccattttcag acactgccga
    48721 taaaataatg gcatcgacat tacgcgccac gagggtatca acaaaggtac tttctttcga
    48781 aatatcaccc tgggcgttag tttcaattaa ctgtacctgt actgaagaat cttttgccgc
    48841 atcctgaacg ccttgacgca ctccagcgta atatccctga gtatcaaggt atattgcgcc
    48901 aatggtcatt tctttttccg cagccctggc aaataaagcg ctacctaata gcgtagccat
    48961 taataataga ttacgggttg ttctcatttt tttaggcata gagcctcctg tagggttttt
    49021 attaacaacg gcttattcta attattttgt gatgagcggc agcgcgtgcc gctcaaatat
    49081 tacagttaat agggtttagt gaacgtagtt aaaaataaaa ggcatcacag tggtgaataa
    49141 tgccgctttc ggccaggccg gattaatcgc cgtcaattca gcgcgagcag actcatattg
    49201 cgcaacctcc ccgagtcccg cggacgccag catcgccacc atcaggcatt tttctttatg
    49261 ctgctgttgt aaatcgccat acagcgataa caggtcaggt tgtgaaacag caaagaaatc
    49321 cgcctcaata ctggttttcg ccatctcttg cgcccactgt ttcatttcac taaacagttg
    49381 ctgtgcggtg tgttgttcgc ccagcagtcg cagcgccatt ccttgccaga agagataatc
    49441 aaccggctga tcgttgtaat aactgtggat gttaatggtg cgatcgccgg tcgccgccag
    49501 acgtaaacaa cgcatcgctt cagtttcatc gccctgcgcg ttggcgcata tcgcctgcca
    49561 gaaccagatg tcgttatcag tttgccccgg taaacggcct tcgcttaaat tctccggata
    49621 atgcagcgcg gcatgaagca gttcgctggc ctgctgcggc tgtctggcat caaggtgctg
    49681 ccaggcgcgt aataactggt tgaggataaa ctgactggtg accttccctt ccccgccttc
    49741 ccacgggtgg aatttgcgcg tggcgagaat gtccgccgct ttgtctgcct gacccgtgag
    49801 atgccacaaa ttgagcaact ctgctgtcat gtcgtcgcgt ttcagcgcaa tttccagatt
    49861 attttccaga cgcgccagtc gtttctccgg tgtggctccg cttaacttat caagcaaatc
    49921 ccgttcgaaa agcagacgtg catcctgcgg cgcaagctga taagcattat caagataacg
    49981 cgcggccagc tcataatcgt gttgcttatt ccacgcatgg atcgctaacc cgcgccagcc
    50041 gtcggcaaac tccggcgaca tctctacgca acgttgccat aaggtaatgg ctttgttgta
    50101 gctacgcttg ttgtagtaga agcaggccag taaatgacgg gcaaaccagc actcttcaat
    50161 actctccagc gccgccactt cttccagcgt attcgggaaa cggacaaact gcgggaagac
    50221 atcaatggct tttgcgacca gttcgccacg ttcggctttc ggcagcaagc tggcttgcag
    50281 gtaaagcggc agcgtgcgct ggcagtccag tgcgttcagc atctctgccg ccagggtggg
    50341 cattccccag ttaatcagtt gcccggcggt catcagggcg ttaacgtcgc gtccctgaca
    50401 cagaccgcgc cactgcgcga gagctgattc gctacgacca tcgaaccagt tcagccacca
    50461 cagagtggcg ttcagcggat aatcgcgcag cagtttctcg cgctgcacac gcgcgttgtc
    50521 ctgacgacca cttaacacca gcagcagatt atgcaggcaa agcacttcct gattggttgg
    50581 gcaggcgcga agactttgtt ggcaaaaatc cagaccagcg tcgaagttac cattacgcgc
    50641 cgccagtcgt gccaggccat aatagccacc ggctttgctg ttgccgctcc agaccgcacg
    50701 ccagaaatcc tcttcggctt gttgatattg tccctgacgt tcgtaagcac tggcgcgaat
    50761 caaactcgcc tgtccgcact gcggattttt gttcagcgca tgtgcgcgtt tcagagcctg
    50821 agtggcatac gccaccgctt gcgggaaatc tgcgcggtta tattccagca tcgccagcgc
    50881 caggttacag cgataatcca gcgggtccag cgccacgccg cgcaggtagt aatcgaacgg
    50941 tgaacggctg gcgtgatgat attgctccag atgctgaccg ataaaccagg cttcatctgt
    51001 actggtaatg tcttgtgctg ccagtggcgc tttggcgacg tccggcaacg gcaactcttg
    51061 cggctgatgt tcctgataac tcaggacaat attgccgtcg gcatcggaga gctcaatggt
    51121 cagcctttca ggattgatac cgtgcaacac gccctggatg gcggtcgcag gcatcagtgc
    51181 cacggcatca tcaagtaacg cgttgcattt gccgatttcg cggatcgcca ggcggtatcc
    51241 gttcaacgga gagatggcat acagccccca ctcaatcccc cgctcactac gctggagttt
    51301 tatcaccgca tcgcgggagg cattttgcac catgcccaaa gaatgataag gcaggaaata
    51361 ctgctcaaaa cgcttctctt cgtaagcatc aagccaggta aaatcaggct ggttatcggc
    51421 aaaaataccg gtcatcagtt cgatatacgg gccgttattg tcggtcagac tcttgtccca
    51481 cgcctggccg aattcactgt gtccccagct ccactgtttt ttacctggcg cgatatggtg
    51541 gttggcaacg tgtagcaaac cgccatcttc atcgtgacac cacgcgccaa caaaatcgta
    51601 ctgtgatttt tcagccatat atgaggttgg aacgggcaca tttttatagc gagaaatgtc
    51661 cactccggcg gagtagtcca ctttgtagta agtgccggtg gcgatgggga aagcggagac
    51721 ggcccgtttg ccgtgatcaa acaccgccgt tacatccggc gggaagacgc tctgatgccc
    51781 ttccccccct ttcactgccg ggttggccca ccacaagaaa tgacgcggcg tggcgttgcc
    51841 gttatagacg cggctggcga tttccaacgc cgcccgttca ggacgcagtg tgaaacctgt
    51901 catcacctgt aaaccgtgca tcggctccgt ttcgccgacc cacaccgtct gtgcgccgtc
    51961 ttcatgggct tcgggggtga aatcaacggg cataaaggtg gtcgggcgat ggtgttgcgg
    52021 ccagttaaac tcaatcccgc cagagatcca cggtcccagc agccccacca gcgcaggttt
    52081 aatgacttca ttgtgataga caaaatcgcg ttgtttcact ttatcccatg cgcgatgcac
    52141 acgaccgccc agttccggca ggatcatcac tttgatgtag tcgttttcca gccacaccgc
    52201 ctgccaggat ttcagggttt tctgctcgct cagcgtatcg gtcacgccgt agggataaac
    52261 cgcgccggac gatccctgat aaacgcgatt ttccaggaac atgggatgta tatcctgcgg
    52321 cccggtttca taggtcggga tctcaacgcg ctcttgccac acttttactg gagtcatggt
    52381 gccctcaata ttaacaagac atactgaatt aaaagatttg tggcagtgta ttgaacaatc
    52441 tggcaatgtt ttcgcggaat aatcacgcaa ttaactaaac aaggtttagt gaagatgaga
    52501 gcctgcatta acaatcaaca gattcgccac cataacaaat gcgtgattct ggaactgctg
    52561 taccggcaaa agcgcgccaa taaatcaacg ctggcccggc tggcgcaaat ttcgattccg
    52621 gcagtcagta atattttgca ggaactggaa agcgaaaaac gggtggtgaa tattgacgat
    52681 gaaagccaga cgcgcgggca tagtagcggt acatggctga ttgcgccgga aggtgactgg
    52741 acgctgtgcc tgaacgtgac gcccaccagt attgagtgtc aggttgctaa tgcttgttta
    52801 agtccgaaag gtgaatttga gtatttacag attgatgcac cgacgccgca ggcgctgctg
    52861 tccgaaatcg aaaaatgctg gcatcgccac cgtaaattgt ggccggaccg caccatcaat
    52921 ctggcgctgg caatccacgg tcaggttgat ccggtgaccg gcgtgtcgca aaccatgccg
    52981 caagcgccgt ggacaacgcc ggttgaggtg aagtatctgc tggaagagaa gctgggcatt
    53041 cgggtgatgg tcgataatga ctgcgtgatg ctggcgctgg cggagaaatg gcaaaataat
    53101 tcgcaggaac gggatttctg cgtgatcaac gttgattacg gcattggctc gtcgttcgtg
    53161 attaacgagc aaatttatcg cggcagtttg tatggtagcg gacagattgg tcacaccatc
    53221 gttaatccgg atggcgtcgt ctgcgactgt ggacgttacg gctgcctgga aaccgtcgcc
    53281 tcgttaagtg cattaaaaaa acaggcgcgg gtatggctaa aatcacaacc ggttaatact
    53341 cagcttgatc ctgaaaaact gacaaccgcg cagttaatcg ctgcctggca gagtggagaa
    53401 ccgtggatca ccagctgggt tgatcgctct gccaatgcca ttggtttgag tctgtataac
    53461 ttcctcaaca tcctcaatat taatcagatt tggatgtacg gtcgcagttg tgcctttggt
    53521 gagaactggc ttaatactat tattcgccag acaggattta acccgttcga ccgcgacgaa
    53581 ggaccgagcg tgaaagcgac gcaaattggc ttcgggcaat taagccgcgc acaacaggtg
    53641 ctggggattg gctatttgta tgttgaggcg cagttacgac agatttgatg gcgcgataac
    53701 cgcgataacg tagaaaggct tcccgaagga agcctttttt caacagatta accgtttcgc
    53761 ttatgcgtaa accgggtaac gtgcgcagat gtcgagaact ttacctttga tgcgctcgat
    53821 aacggcttca tcattgatgc tgtccagcac gtcacacatc cagccagcca gttctttcgc
    53881 ttctgcttct ttgaagccgc gacgcgtaat cgccggagta cccacgcgaa taccggaggt
    53941 cacaaacggg ctcttcggat cgttcggtac gctgtttttg ttgacggtga tgttagcacg
    54001 gcccagagcg gcgtctgctt ctttaccggt caggttttta tctaccagat caaccaggaa
    54061 cagatggtta tcagtaccgc cggaaaccac tttgtagccg cgttccagga acacttctac
    54121 catcgcttta gcgtttttag cgacctgctg ctggtaagtt ttgaactcag gctccatcgc
    54181 ttctttcaga gcaaccgctt taccggcgat tacgtgcatc aacggaccgc cctgaccacc
    54241 agggaaaacg gcagagttca gttttttgta cagctcttcg ctaccacctt tcgccaggat
    54301 caggccgccg cgcggacccg ccagggtttt gtgagtggtg gtagtaacaa cgtgagcatg
    54361 aggaaccggg ttcgggtaga cgccagcagc aaccaggccc gcaacgtgcg ccatatcaac
    54421 gaacaggtaa gcaccgatgc tgtcagcgat ttcacgcatt ttcgcccagt ccaccacgcc
    54481 ggaatatgca gagaaaccac cgataatcat tttcggcttg tgttctttgg cttgtttttc
    54541 cagatcggcg tagtcgatat gaccggtagc atcgataccg taaggaacga tgttgtacag
    54601 tttaccggag aagttaaccg gagaaccgtg agtcaggtga ccgccatgcg ccaggttcat
    54661 acccagaacg gtatcgcctg gttccagcag cgcggtgtag accgcaaagt tagcctggga
    54721 gccggagtgc ggctggacgt tagcgtagtc agcgccgaac agttctttcg cacgatcgat
    54781 cgccagttgt tcaacgatat caacatactc gcaaccgccg tagtagcgtt tgcccggata
    54841 accttcagca tatttgttgg tcagctgaga accctgcgcc tgcattacgc gcgggctggt
    54901 gtagttttcg gaggcgatca gttcgatgtg ctcttcctga cgtacttttt cctgctccat
    54961 agcctgccac agttcggcat cataatcggc aatgttcatt tcacgcttta acatccgcat
    55021 ctcctgactc agctaacaat aaaatttttg gcctttatag gcggtcctgt tggacaacgg
    55081 cgaacagtat aaccgaatca ttgtgcgata acaggtcttg acaaaggaat ttacgcaaac
    55141 gattaccttc aggctacgca aggctttgga gaataaagag cttgcaaccg gaaacggatt
    55201 tcttttcagg tttgtgatgc aaatttttca cttcatcaca ttctttctga aaaacaccaa
    55261 agaaccattt acattgcagg gctatttttt ataagatgca tttgagatac atcaattaag
    55321 atgcaaaaaa aggaagacca tatgcttgac gctcaaacca tcgctacagt aaaagccacc
    55381 atccctttac tggtggaaac ggggccaaag ttaaccgccc atttctacga ccgtatgttt
    55441 actcataacc cagaactcaa agaaattttt aacatgagta accagcgtaa tggcgatcaa
    55501 cgtgaagccc tgtttaacgc tattgccgcc tacgccagta atattgaaaa cctgcctgcg
    55561 ctgctgccag cggtagaaaa aatcgcgcag aagcacacca gtttccagat caaaccggaa
    55621 cagtacaaca tcgtcggcga acacctgttg gcgacgctgg acgaaatgtt cagcccgggc
    55681 caggaagtgc tggacgcgtg gggtaaagcc tatggtgtac tggctaatgt atttatcaat
    55741 cgcgaggcgg aaatctataa cgaaaacgcc agcaaagccg gtggttggga aggtactcgc
    55801 gatttccgca ttgtggctaa aacaccgcgc agcgcgctta tcaccagctt cgaactggag
    55861 ccggtcgacg gtggcgcagt ggcagaatac cgtccggggc aatatctcgg cgtctggctg
    55921 aagccggaag gtttcccgca tcaggaaatt cgtcagtact ctttgactcg caaaccggat
    55981 ggcaaaggct atcgtattgc ggtgaaacgc gaagagggtg ggcaggtatc caactggttg
    56041 cacaatcacg ccaatgttgg cgatgtcgtg aaactggttg ctccggcagg tgatttcttt
    56101 atggctgtcg cagatgacac accagtgacg ttaatctctg ccggtgttgg tcaaacgcca
    56161 atgctggcaa tgctcgacac gctggcaaaa gcaggccaca cagcacaagt gaactggttc
    56221 catgcggcag aaaatggcga tgttcacgcc tttgccgatg aagttaagga actggggcag
    56281 tcactgccgc gctttaccgc gcacacctgg tatcgtcagc cgagcgaagc cgatcgcgct
    56341 aaaggtcagt ttgatagcga aggtctgatg gatttgagca aacaggaagg tgcgttcagc
    56401 gatccgacaa tgcagttcta tctctgcggc ccggttggct tcatgcagtt tgccgcgaaa
    56461 cagttagtgg atctgggcgt gaagcaggaa aacattcatt acgaatgctt tggcccgcat
    56521 aaggtgctgt gatttgatgt tgccggatgg aaacatccgg caacccttga cgcggtttaa
    56581 attgccgcgt cgtcctcttc accggtacgg atgcgaatga cccgtgccac gtcaaagacg
    56641 aagattttac cgtcaccgat tttgccggtt tgcgccgtgc gaataatggt atcgacacag
    56701 gtatcgacaa tgtcgtccgg tacgacaatc tcaattttca ctttcggcag aaaatccacc
    56761 atatactccg cgccgcggta cagctcggta tggcctttct ggcgaccaaa gcctttcact
    56821 tcggtcaccg tcatgccggt aataccgact tcggccagtg cttcgcggac atcgtccagc
    56881 ttgaagggtt ttataatcgc atcaatcttt ttcatgctat tccttgaaaa ggtcgcctgt
    56941 cttttgatct gctaaacgta acacataacg ccaattcatt ccttgaaatc gtttgcatcc
    57001 agctcgtgtc gggaaagcag tttataaaat tctgtccggt tgcgccccgc cattctcgcc
    57061 gcgtgggtga cgttgccttt ggtgatttgc agcagcttac gcaaatagtt gagttcaaac
    57121 tgattacgtg cctcaacaaa ggttggcagc gccgtatttt caccctccag cgcctgctct
    57181 actaatgcat cgctaataac cggagatgag gtcagcgcca cgcactgttc aatcacgttg
    57241 accaactggc gcacattacc cggccagctc gcggtcatca ggcgtttcat cgcatcggta
    57301 gagaacgcgc ggacaaacgg tttatgtcgc tctgccgcct ggcgcaacag gtgatttgcc
    57361 agtagcggaa tgtcttctgt gcgctccgcc agtgccggaa ttttcaggct gacaacgttg
    57421 aggcggtaat agagatcttc gcggaattcc ccgcgcgcca tcgcttttgg cagatcacgg
    57481 tgagtggcag aaataatccg cacattgata tcaatatcgc ggttactgcc cagcgggcgc
    57541 actttacgct cctgcaacac gcgcagcagt ttgacctgta acggcgcagg catatcgcca
    57601 atttcatcga gaaatagcgt tccgccttcc gccgcctgga ataaaccttc gcgattgctg
    57661 acagcgccag taaacgcgcc acgcgcatga ccaaacagct ccgactccag caattgctcg
    57721 ggcaatgcgc cacagttaat agcaataaat ggtttgctgt tgcgcgggct ggcgttgtgg
    57781 atagcctggg cgaaaatctc tttcccggtg ccgctctgac cgttaatcaa aacgctgacg
    57841 tctgattgcg ccaccagccg cgcctgttcc agcaaacgca gcatcagcgg gctgcgggtg
    57901 acaattgcct cgcgccagcg ttcatcggtg gctggcgcgg attgctccag cgcatcgtca
    57961 attgcctgat atagcgcgtc tttgtcgaca ggcttggtga ggaaactaaa aacgccctgc
    58021 tgtgttgcag caacggcatc ggggatagaa ccatgcgcag taagaataat tactggcatt
    58081 cccggctgca ctttctggat ttcagcaaat agctgcatac cgtccatttc atccatccgc
    58141 aggtcgctga taactaaatc tactttttcg cgattcagta cccgtaatcc ttcagcgcca
    58201 ctttccgccg tgaccacact gtagccttcg ctggtcaggc gcaggccaag cagtttcagc
    58261 aatcccggat cgtcatcgac caataataaa tgcgcaggtt tatggctcat caggagtgac
    58321 ctcatgggtg gatggcgcgg gcttttcact ctcgtgcggc gtatccggcg agaaatttcc
    58381 agccggtttg cgggtcgaga gctggcgttc aatatcggtc aggttttcca gcttgcgggt
    58441 ggtgagttcc agttgctgct gtagaacgtg atgttgctgg cgcaatgtat ccagctcgct
    58501 gtcgctggac tgctggagtt tactgtaacg ttggcgctct tccgccagtt gcagttgcag
    58561 cgcctgaccg tcgcgccaga gttgatacag tgggcgaacc tgtgccggga tctcggtact
    58621 taacgcttca atacgcgcga ccagctggcg gcgctcatac ggcgtaattt tggcgtcggc
    58681 gagcaaaatc ccttgtttaa aggtattttg ccagctgccg tcgtcatatt ggcgagcttg
    58741 ctgacgcgac tgcgcaggca ttaaacgatc agcacaatcc atcgcccgca gccagtaaag
    58801 cggattggtt tcggttgatt taccttgcag cgcccagatg tcgctacatt cagtagaaag
    58861 atagtcagcc agttgataaa ccggaatttt ttcttctgct ggcgtatcaa tggctggctt
    58921 attgtgattc tgcacgcaac ccagcaatgc cagacatggc agccctgcca gccacagccg
    58981 tcggggcaat aatcgttgaa aaatgtgtcg catattcacc agacttaaag cctatcccag
    59041 tgggcgtaat tgttgcagac agtctggaca tggacagcgc ggagaaaccg gagcgtacat
    59101 atcatacgtg aagatttcga gcactgcccg gggccgaact gacaaataaa atagcctgat
    59161 aggataggct gttagcatta tttcgtgttt ttcgacgacg gtaattcaat gcggaaacaa
    59221 acgtcttgcc cgctctcgtc gaccagatac agttcccctt gcatacggcg aatacaatcc
    59281 ctggcaatgc ttaatcccag accgctgccc ttcaccgccc cttttcgctg gtggcttccc
    59341 tgaaaaaagg gttcgaagat catggcgcgt tcctcttgcg gaatgggcgt gcctgtattg
    59401 atgacatcaa tataaacccg cgcaccatgt gaactgctgc gaaggcaaat gttaccggat
    59461 tcagccccgt agtgcaccgc attggagtaa agattatcca gtacgctcat cagcagcatt
    59521 ggctccgcca ggcaagctgt tgctttgaga tcgacgtcgg tatgcatcat tttagcccgt
    59581 gcgggcaggc tatgagcaga aaccactgtc tccaccagcg gtgctaattc aacattctcc
    59641 agttccaccg cactgtccgc ctgtttacgg ttgtaatcaa gcagttgttc gatcagtttt
    59701 tgcaaattgc ggctgctgct atcaagaatg ctcaccacct ctttttgctc tggcgtaagc
    59761 ggcccgacaa cctggtcagc cagtaattca gtgccctcgc gcatactcgc cagtggcgtt
    59821 tttaattcat gagataaatg tcttaaaaat tgatggcgtt gggattccag ccatgacagg
    59881 cgctcactta accaaagaat acgttgccca accgagcgta actcgctcgg tccactgaac
    59941 gaaacgctat tgcccagaga acgcccttcc cccagacggt tgatcatgcg ctcgatattt
    60001 ttcaccggcc cgataatcat ccgcgtgaaa agtagcacca tcaccagact caccagaaat
    60061 agcaccagcg attgccaacc aaaatattgc ccacgttcgg cgatttcacg ctgaagttgc
    60121 tgcccacgag agaacaccac tgtgcgcgtg gcctgcacca tttcggtatt ggcactggca
    60181 aaggcttcaa gacgcgcggc ggcggcagca tcgggaccac tgttgttaca ctgaagttgg
    60241 gccagattgt tcaaatcctg acgcaatgcc tggtaaagtt tatcgtctgg cagtacgcct
    60301 gcgtgggcat cgagcatttc gctgtaacgc ttgcgctggc tttgataaac cttcgcaagc
    60361 gtagggtcgt ccagcacgca atactgacgg tagctacgct ccatctccag cgccgcgttg
    60421 gtcatcgctt cactacgccg ggcatcaata agcgtagtgc ggttaaccag cgccgcctga
    60481 tcgctaagcg cattcaggct ttgccaggct tgccatgcca gcaccaacaa gggcagcaga
    60541 attagcaaaa atgccagcat taccagttgt cgtaatgagc ggggaaaaac gggccagcgt
    60601 ttcaaggtgt tactctcgtc agacgcgaat agcctgatgc taaccgaggg gaagttcaga
    60661 tacaacaaag ccgggaatta cccggctttg ttatggaata aggcggtgcc taactcgacg
    60721 tttcgcccga tggttgatat agctacgctg atatcagaag ttggacggca ggcaccttgt
    60781 tgtgcgtcat tcgaatttta tgtagcacgt cccgaagggg ctgacataag tcggtgaatg
    60841 agccactgct tactattatg cagtaactgt gccaataaag aaaatagttt ggtaacgtac
    60901 tgattatacg ttgctttgag ggatttatgg cttcttcgct atttgaatgt aaatcagcca
    60961 ctgtgtcgcc aaaaagagac aacttaagat aaacttatta gattacaata aaatcaatca
    61021 tttaagtgtc gccagaaagc gacacggtaa accacccatt gtcgtgattt acagacacaa
    61081 aaaagctccc ggagttggga gcttatgata gtggttggtg cttaatgccg catccgggct
    61141 gcaaaaccaa tgggctgacg acttacccca actgcttacg cgcattgcgg aaaatacgca
    61201 tccatgggcc atcctcgccc cagttttccg gatgccagga gttgctgaca gtacggaaaa
    61261 cacgttccgg gtgcggcatc ataatggtga ctcgaccact ttcagtcgtg actgccgtaa
    61321 taccgttcgg tgaaccgttc gggttagccg ggtaggtttc agtgactttg ccgaagttat
    61381 cgacatagcg cagtgccacc agccctttgc tttccagtgc cgccagatgc gccgcatcac
    61441 gcacttccac gcgcccttca ccatgagaga cagcaatcgg catttgcgag cccaccatcc
    61501 cctgcaacag cagcgacggg ctttgggtca cttcaaccag gctgaaacgc gcttcaaagc
    61561 gatcggaggt attgcgcaca aaacgtggcc acaactcact acctgggatc agttcgcgca
    61621 ggttagacat catctggcaa ccgttacata cccccagcgc cagcgtttgc ggacggtgga
    61681 agaaggttgc aaactcatcg cgtacacggt cattgaacag gattgacttc gcccaacctt
    61741 caccggcacc cagcacatca ccgtaggaga aaccaccgca cgcgaccagg gcgtggaaat
    61801 cttccaggcc cgtgcgtccg gtcagcaggt cactcatatg cacgtcgata gcatcaaagc
    61861 ctgcacggtg gaaagctgcc gccatttcaa catgcgagtt cacgccctgc tcacgcagta
    61921 cggcaacttt cggacgtgcg ccagtggcaa tatacggtgc tgccacatct tcgttgatat
    61981 cgaacgacag ttttacattc aggccaggat cggcatcgtt agatttcgcc tggtgctcct
    62041 ggtcagcaca ctccgggttg tcacgcaggc gctgcatctg ccaggtagtt tctgcccacc
    62101 agacacgcaa cgtggtgcgg ctttcgctga atacagtctg cccgttggcg gtaatcacaa
    62161 aacggtcacc ggaaaccgcc tgccctacat aatggacaca atcagcaagc ccatgctgtg
    62221 ccagtacgga ctcaaccgct tcacggtcag cggcacgaac ctgaatcacc gcacccagtt
    62281 cttcgttaaa caacgccgcc aggcgatcgt cgcccagaga ggcaatatcc gcgttaatgc
    62341 cgcaatgacc agcaaaggcc atttccgcca gcgttaccag caggccgcca tcagagcggt
    62401 cgtggtatgc gagcagctta cgctgtgcaa ccagcgcctg aatcgcgtca tagaagcctt
    62461 tcagttgcgc gacgtcgcgt acatctgccg gtttgtcgcc aagctgacga taaacctgcg
    62521 ccagcgccgt tgcgcccagc gcgttattgc ctttgcccaa atcaatcagc agcagtgcgt
    62581 tatcttcggt agaaagctgt ggcgtgatgg tgtgacgcac atcttccacg cgggcaaatg
    62641 cagaaatcac cagcgacagc ggcgacgtca tttcgcgctc ttcgttacct tcctgccagc
    62701 gggttttcat cgacatagag tctttaccca ccgggatcgt caggcccagc gccggacaaa
    62761 gctcttcgcc cacggcttta acggcttcat acaggcccgc atcttcacca gggtggcctg
    62821 ccgccgccat ccagttggcg gaaagtttaa tgcgtttgat atcaccaatc tgtgttgcgg
    62881 caatattggt taacgcttca ccgaccgcca gacgggcaga ggcggcgaaa tccagcagcg
    62941 caaccggcgc acgctcgcca atcgccatcg cttcaccgta gtagctgtcg aggctggcgg
    63001 tagtgaccgc gcagttagcg accggcacct gccacggccc caccatctga tcgcgcgcta
    63061 ccatgccggt tacgctgcgg tcgccaatgg tcaccaggaa ggttttttcc gccacagtcg
    63121 gcagatgcag cacacgtttc accgcatctg caatggtgat cccttcacgg gccagcgcgt
    63181 cgcctttcgc tttcagcatt tgtacatcgc gggtcatctt cggcgtttta ccaagcagga
    63241 cgtccagcgg cagatcgatc ggctgattat caaaatgacg atcgtgcaga gaaagatgca
    63301 gttcttcggt cgcttcacca atcaccgcgt agggtgcgcg ttcacgctta cacagttcgt
    63361 caaacagcgg taattgatcg gcagcaatcg ccagcacgta gcgttcctgg gattcgttac
    63421 accagatttc cagcgggctc atgcccggtt cgtcgcttag aatatcgcgc agttcaaatt
    63481 taccgccgcg tccgccgtcg ctcaccagct ccggcatcgc gttagaaaga ccgcccgcac
    63541 cgacgtcgtg gataaacaga attgggttag catcaccaag ctgccagcaa cggtcgatca
    63601 cttcctggca acgacgctcc atctccgggt tgtcgcgctg tacggaagca aagtcgaggt
    63661 cggcatcaga ctgacctgac gccatggaag acgccgctcc gccgccaaga ccgatgttca
    63721 ttgccgggcc gccgagaacg accagcttcg caccgacgtt gatctcgcct ttttgtacgt
    63781 gatcggcgcg aatgttgccg atcccgcccg ccagcatgat cggtttgtga taaccgcgca
    63841 gctcttcgcc gttgtggctg ttcacttttt cttcataagt acggaagtag ccattcagtg
    63901 ccggacgacc aaattcgttg ttaaacgccg cgccgcccag cgggccttcg gtcatgatgt
    63961 ccagcgcggt gacaatgcgc tcaggcttac cgaaatcttc ttcccacggc tgttcgaagc
    64021 caggaattcg caggttggaa acggagaaac caaccaaacc cgctttcggc ttcgcgccgc
    64081 gcccggtggc accttcatcg cggatttcac cgccggaacc ggtcgccgcg cccggccacg
    64141 gagaaatcgc cgtcgggtgg ttgtgagttt cgactttcat cagaatatgc gccggttcct
    64201 gatggaaatc gtagcggccc gtttcgtggt cagcaaagta gcggcccact tcagaacctt
    64261 ccattacggc ggcgttatct ttataagcag agagaacgtg atctggcgtg gtttcgaaag
    64321 tatttttgat catcctgaac agcgatttcg gctgttgttc accgtcgata atccagtcgg
    64381 cgttaaaaat tttgtggcgg cagtgctcag agttcgcctg agcgaacata tacagctcga
    64441 tgtcgttcgg gttacgacca agctttgtga aagcatcctg cagatagtca atttcatctt
    64501 ccgccagagc caggccaaga cgcaggttag cgtcgatcag cgcctgacgg ccctgcccca
    64561 gcaaatcaac gcttgtgacc ggagtcggtt gatggtgggc aaacaactgc tctgcatcat
    64621 ctaaatcaaa aaagaccgtt tccatcatgc ggtcgtgcag ttcagcggta acctgctgcc
    64681 attgttcatt ggtcagcgta ccggcttcta tatagtaagc aacgccgcgc tcaaggcggt
    64741 ttacctgttg tagcccgcag ttatgggcaa tatcggtcgc tttcgaagac cagggagaga
    64801 tggtgccagg acgcggggtc accagcagga gtttgccttg cggggcgtgg ctggcgagtg
    64861 ccggaccata tttcagcagg cgttcaagtt gtgcgtgctc atcatcgttt aacggcgcat
    64921 tgaggtcagc aaaatggaca tactcggcgt aaatattgtg aaccgggagc ctggcagcct
    64981 gaaaacgtgc cagcagtttg ttgattcgga atgccgacag tgcaggcgaa ccacgcagaa
    65041 tttccatcat aagtctctcg tcttctaagc tttcggtgta cccaaggggg ggaaacgggc
    65101 gtcattataa agaatctgat gcgctgacga aaccgtttgc gtggaaataa aatcaccatc
    65161 gtgaattagc aacgcgtgcc gccaatggat gtaataagtt gccatctggc gcaggtttac
    65221 gcaaaatgcc gctcatttat gagtaaacct ttcactatta ttacgttttt tcaagctggg
    65281 acgcgcacga cacagagaat taactaattg aaaaaattaa agattaatta tctgttcatc
    65341 ggcattctgg cactgctgct cgcggtcgct ctctggccat ccattccctg gtttggtaaa
    65401 gccgacaacc gtatcgccgc cattcaagcg cggggagagt tgcgtgtgag caccattcat
    65461 actcccctga cttataacga aatcaacggg aaaccttttg gcctggatta cgaactggcg
    65521 aaacagtttg ccgattacct cggcgtaaaa ctgaaagtga ccgtgcggca gaatatcagc
    65581 cagctgtttg acgaccttga taatggtaac gccgacctgc tggcggcagg acttgtctat
    65641 aacagtgagc gggtaaaaaa ttatcagcct ggccctacct attattccgt gtcacaacaa
    65701 ctggtttata aagtgggtca gtatcgccca cgtacgctgg gcaacctgac ggcggagcaa
    65761 ctcaccgttg caccgggtca tgtggtggtt aacgatctcc agaccctgaa agaaacaaaa
    65821 ttcccggaat taagctggaa ggtagacgac aaaaaaggct ctgcggaatt aatggaagat
    65881 gtcatcgaag gaaaactcga ttacaccatt gctgattctg tcgccatcag cctgtttcag
    65941 cgcgttcacc cggagctcgc cgtagcgctc gatatcaccg atgaacaacc ggtgacttgg
    66001 tttagcccgt tagatggcga taataccctt tccgccgccc tgctcgactt cttcaacgaa
    66061 atgaatgaag acggtacgct ggcacgcatt gaagagaaat acctggggca tggcgatgat
    66121 tttgattacg tcgatacgcg cacattttta cgcgccgtcg atgcggtact gccgcagtta
    66181 aagcccctgt ttgagaaata cgccgaagaa attgactggc gtttgctggc cgctattgct
    66241 tatcaggaat cgcactggga tgcacaggcc acttcaccga cgggtgtgcg cggcatgatg
    66301 atgttaacca aaaataccgc gcaaagcctc ggcattacgg atcgtaccga tgccgaacag
    66361 agcatcagcg gtggcgtgcg ttatttgcag gatatgatga gtaaagtgcc ggaaagtgtg
    66421 ccggagaacg agcggatctg gtttgccctc gctgcgtaca atatgggcta tgcgcatatg
    66481 ctggatgccc gcgccctgac ggcaaaaacc aaagggaatc ctgacagttg ggctgacgta
    66541 aaacagcgtc tgcctttact tagccagaaa ccctattaca gcaagctgac ttacggctac
    66601 gctcgtggac atgaagccta cgcttatgtc gaaaatattc gtaagtatca gattagcctg
    66661 gtgggttatc tgcaagagaa agagaagcag gctacagaag cggcgatgca actggcgcag
    66721 gattatccgg cggtatcgcc tacggagttg ggcaaagaga aatttccttt tctctcgttt
    66781 ctttcccagt cgtcatcaaa ctatttgacc cattctccct ctctgctgtt ttccaggaaa
    66841 gggagtgaag agaaacaaaa ttaatccgtc gaggattgcg cttttttctg cgctttaatt
    66901 tcctggcggc gcatgcgaaa gaagtcactg agcaacgccg cgcactcatc cgccagtatt
    66961 ccttccgtaa tttccactcg gtgattcata cccggatgat gcagcacatc cattaaagat
    67021 cccgcagcgc cagttttcgc gtcacgcgca ccaaagacca cgcgaccaat gcgactgtgg
    67081 atcatcgctc cggcacacat tacacatggt tcaagcgtga catacaacgt ggcgtcgatc
    67141 agacgataat tttgcatcac cagaccaccc tgccgcaggg ccatgatttc tgcatgtgcg
    67201 gtgggatcat ggcgaccaat cgggcggttc cagccttcgc cgattacccg attgttatgc
    67261 actaataccg cgccgaccgg cacttcccgc tcatcccagg cacgtttcgc cagcgtcagc
    67321 gcgtgacgca tccagtattc gtggctaaat tcgacttcag acaaaaagaa aactccggtt
    67381 ataaaagcgc ggcgcattat acacggacgc tatgctttac tctattccag ttgctggagt
    67441 tcaccgcgcg gggttactcg ccaacgatgc tgacagaaat aaagcagcgg attgtcctga
    67501 ttactgtcgc tatagccact gtacagccgc agcggagtgc cgattttgcg ctccagttgt
    67561 gcgacctttt catgtcccag acaacgcatc gtcaataccc aaccaccata gccacgctga
    67621 atttggctgg cgataagatt aacccgcggc agccagggcg tatcgaaata aaccgcttca
    67681 accagcggct gcggagagcc ggtaatcaac cagatatcag catcggaact taacaggtag
    67741 gtggttaatc gctcctgaac cagcggaaag gcggtaacat tgtcgcgaaa ccagcgcacg
    67801 aaatcggcct gcaacgtctg taaacgtgct tcgctgtgac caaaagtgca cccccacaga
    67861 agcagactca tcggccagcg tgccgcacga ccttttatca ataacgcaat ggctataatc
    67921 ggtaacaacg gcaggacaag taacgcattc agcggttggc gacgcagtaa atagcgcaga
    67981 aaactgccga acatatcctg ctgatgcaat gttccatcta agtcaaaaaa caccacacga
    68041 cgctcgtgag ttgccaaacc atactcctct ggactgaaac atcctgatta attttcgcta
    68101 ctacatagcc taacagatag atcatcactt ttccggcaac aatcgaccgc atcatggcta
    68161 actggaattt ttaattcatg gcaattagcg gcaatggaat ataaaattca ctcgcgtgtc
    68221 tctcatattg cctgttgtcg ccactatttc atcgcgagga aagatatgaa cggcttactt
    68281 cgtatccgtc agcgttacca ggggcttgcc cagagtgata aaaaactggc ggattatctg
    68341 ctgctacaac ctgatacggc gcgccattta agctctcagc aactggccaa cgaagccgga
    68401 gtcagtcagt ccagcgtcgt gaagttcgcg caaaaactcg gctataaagg ttttccggcg
    68461 cttaagttgg cgttgagtga agcgctggca agccagccgg aatcgccctc cgtgcccatt
    68521 cataaccaaa tccgcggtga tgatccgtta cggctggtcg gcgaaaaact gattaaagaa
    68581 aatactgccg ctatgtacgc aacgctaaac gttaatagtg aagagaaact gcatgaatgc
    68641 gtaacaatgt tgcgctctgc gcggcggata attctgaccg gtattggcgc ttcgggtctg
    68701 gtggcgcaaa actttgcctg gaagctgatg aagattggct tcaatgctgc cgcagtgcgc
    68761 gatatgcatg cgctactcgc aacagtacag gcgtcgtccc ctgacgatct gttattagcc
    68821 atttcctaca ccggtgtacg acgcgagtta aacctggcgg cagatgagat gctgcgagtg
    68881 ggcggaaaag tgctggcgat taccggcttt actccgaatg ccctgcaaca gcgtgcttct
    68941 cattgcctgt ataccattgc cgaagaacaa gcgacaaaca gcgcttcaat ctctgcttgt
    69001 cacgctcagg gaatgttaac ggatttgctg ttcattgcgc tgattcagca ggatctggaa
    69061 ctggcaccag aacgtattcg tcatagtgaa gcgctggtga aaaaactggt ctgagtaaag
    69121 aatgcgcgta taatgcccgc ccggtttgtg ttgttttgag agtttcctta tggcgttgtt
    69181 aatcactaaa aaatgcatca attgtgatat gtgtgaaccc gaatgcccga atgaggcgat
    69241 ttcaatggga gatcatatct acgagattaa cagcgataag tgtaccgaat gcgtagggca
    69301 ctacgagaca ccaacctgcc agaaggtgtg cccgatcccc aatactattg tgaaagatcc
    69361 ggcgcatgtc gagacagaag aacagttgtg ggataaattt gtgctgatgc accacgcgga
    69421 taaaatttaa tattctactc tggaagtaga gtattaatta tattactggg aagccttaac
    69481 gccattatat ttatttaatt gatgacatta gcataatcat tcactaagtt aatttatata
    69541 gtatctgcca agacacttat ttatagttat taaaggcgcg tccgattggt tcaccggacg
    69601 caccttaagt acgtttcctt gtgttataag aacagaagga tcagctgtaa aacagcaatg
    69661 atgattttga tgacccgttt aatcaggtat cggcaatcag tcattcgttt ttccttaaac
    69721 aaggaatgca gccgttcaga ttagccctta catctcccca aaactgaacg tgcgagttat
    69781 tgagggtgca tgctgcactc cacaccagag ctttgacgac accactcgtt tcaatggggg
    69841 aattctgtgg catggtgtaa agcacagcaa aatcttcaat aacgaagcca attttaatgt
    69901 acttacgaat tggcggtcac gttaatcttt ccatcaatat tgctttcttc gtaaaggctc
    69961 gagtttttat gctaaagatt gcaagttgct tgtaaaagat aagtacactg atccataatc
    70021 gctgttgttg agggtgcatg ctgcacaaaa ttaaagttaa aaagtaaaac ccccgttcct
    70081 taccagttcg ggggttttac tttttaaaga gaacggtatt atttttaact ttcaataatt
    70141 accgtggcac aagcatagtg ccgctcatct gccagcgtta catgcatatt tgcaacgccc
    70201 agcttttccg ccagttttaa tgcctcgccc catagccgta gccgtggttt gccgagctca
    70261 tcattgaata cttcaaattg attaaacgcc agaccattgc ggatcccggt gccaaacgct
    70321 tttgctgcgg cttctttcac agcaaaacgc ttcgccagaa aacgcaccgg ctggtggtgc
    70381 gttttccaga tagcccattc gttatcgctt aatacgcggc gtgccaggcg atcaccggat
    70441 cgggcgatca ccgcttcgat gcgagcgatc tccacaatat ccgtgcctaa acctaatatt
    70501 gccattagcc acgcgcttcc agcatcagac gcttcatttc tgccaccgca tctttcagtc
    70561 cggtcatcac tgcacgacca ataatggcat gaccgatatt cagttcatgc atctcaggga
    70621 tggcggcaat ggctttcacg ttgtgatagg tcagaccgtg tccggcgtta actttcagac
    70681 cgaggcttgc ggcaaaggtc gcggctttgg cgatacgcgc cagctcttgc gcctgttcgg
    70741 cgtcagtttt ggcatcagca tagcaaccgg tgtggatctc gataaacggt gcgccaacct
    70801 ctgccgcagc tttgatctgc tcttcatcgg cgtcaataaa cagagaaacc tgaatcccgg
    70861 catctgccag acgtttgcag gcatcgcgca ttttgtcacg ctgccctgcg acatccaggc
    70921 cgccttcggt tgttacttcc tgacgctttt ccggtaccag gcagcaaaaa tgtggcttcg
    70981 tctcaacggc gatcgccagc atctcttcgg tcaccgccat ctccagattc atgcgggtat
    71041 ccagcgtctg acgcaggatg cgcacgtcgc ggtcagtaat gtgacggcga tcttcacgta
    71101 aatgcacggt aatgccgtcc gctcccgcct gctcggcaat aaacgcggcc tgcaccggat
    71161 ccgggtaagc ggtaccgcgc gcgttgcgca gcgtagcgat atggtcaatg ttgacgccta
    71221 acagtaattc agccatgaca atcctcatca ttcataatgt gttttcaccg ttcgcttagg
    71281 cataaattgc cggaacagtt ccctgctctt taaaggttta ccgccaagat acggcttaag
    71341 cgccatgcgg gtaaagcgtt tcgcagcgcg cagtgtgtct gcgtcaggaa attcccgtgc
    71401 gtttaacgct tttaactgcc ttccggtgaa cgttttattg tcgataacga cgcttgcgat
    71461 aaaccctttt tcttcgcgat aacgatacgt catggtgtca tctaccggct cgccgctacc
    71521 cgcacaatgg gtaaaattga cgccataacc cagatgcccg agcagtgcca gttcaaagcg
    71581 gcgcagcgcg ggttctggcg taccagtgcc ccctgcaaga gactgaatgc agtgcaagta
    71641 atcgaaaaaa agttcagaga agcgcgtctc gtattccagt acgcgggaga gaagttcgtt
    71701 gatgtacaga ccgctgtaaa gcgtgatacc gcttaatggc agcgccagcg agacggcttc
    71761 agcactgcgc agcgttttga cttcgccacg cccgccaaaa cgtagcaaga gaggggtgaa
    71821 aggctgtaat gcacctttca gggtagagcg tttagagcgt gcgcctttgg caaccagacg
    71881 cacgcgcccc gattcctccg tgaagacgtc cagcatcagg ctggtttcgc tccacgggcg
    71941 actatgcagg acaaatgcgc gctgccagcc ttccatcgga gttactctta aagatcgtca
    72001 acgtaaccga gactgcgcag tgcgcgttcg tcgtcggccc aaccggattt cacttttacc
    72061 cacagttcaa ggtgaacagg cgcttcgaac atttcctgca tgtctttacg cgcttcaatc
    72121 ccgatggttt tgatcttggc ccctttgttg ccaatgacca tcttcttctg cccttcacgc
    72181 tcaacgagaa tcaaaccgtt gatgtcataa ccaccgcgtt cgttagagac gaaacgttcg
    72241 atctccacgg tcacggagta cggcagttca gcgccgagga aacgcatcag tttttcgcgg
    72301 atgatttcag acgccataaa acgctgtgag cgatcggtga tgtaatcttc cgggaagtga
    72361 tgagtcgctt caggtagatg cttacgcacg attgccgcaa tagtgtcaac attcagcccg
    72421 gtttcggcag agattggcac gatatcgagg aagttcatct ggcttgccag gaactgcagg
    72481 tgcggcagca gatcggcttt ctcctgcacg ttgtccactt tgttcaccgc gaggattacc
    72541 ggcgctttgc cttcgcgcag tttgttgagc accatttcgt cgtccggcgt ccagcgggtg
    72601 ccttcaacga caaaaatcac cagctcaaca tcgccaatag agctgctcgc cgctttgttc
    72661 atcaggcggt taatggcgcg tttttcttcc atatgcaggc ccggtgtatc gacgtagatc
    72721 gcctgatacg cgccttcagt atggatcccc acaatgcggt gacgagttgt ctgcgccttg
    72781 cgggaagtga tggagatttt ctgccccagc agtttgttca acaatgtgga tttgccaacg
    72841 ttcggacgtc cgacgatggc aataaatccg cagtaacttt tatcgatgct cattccagct
    72901 ccagtttttt caacgcctgt tcggcggcag cctgctcagc cttacgacgg cttgaacctg
    72961 tgccaaccac cggttcactc aggccgctga cctggcagtg gatagtaaat tcctgatcgt
    73021 gcgcttcgcc acgtacctgg actaccagat aagtcggcag cggcagatgg cgaccctgca
    73081 aatattcttg caagcgcgtt ttcggatctt tttgtttatc gcctgggcta atttcgtcca
    73141 aacgagtttg ataccagttg aggattaatt tctcgacggt ttgaatatca ctgtcgagga
    73201 atacgccacc aattaatgct tcgacggtgt cggcgagaat tgactcacga cgaaatccac
    73261 cgcttttaag ttcacctggc cctaaacgta agcactcgcc taactcaaat tcgcgcgcca
    73321 gttccgccag cgtattgcca cggaccagcg tggcgcgcat ccggctcata tcgccttcat
    73381 ccacacgagg gaaacggtga taaagcgcat tggcgataac gtagctcaga atagagtcgc
    73441 ctaaaaattc taaacgctcg ttatgtttac tgctggcact acgatgagtt aatgcctgct
    73501 gcaacagttc ctgatgatta aaagtgtagc ccagcttccg ttgaagccga ttaattacga
    73561 tggggttcat gcgataccaa taaataaatg cgtcaacaat tcagcacacg aaacagacct
    73621 gatatacatg gttctgctaa ctgcttcgct gcagtttcag tttaccggta tatggggacc
    73681 aacgctgttt cgtgtgccgt ggcaacctgg aggtgccaac cttaaacttc gggggaatat
    73741 tctatacaca acgacggggg atgtcgttag ccacgggaga tttatctcat aaataattca
    73801 cgttgtcgcc ataacggcga caacgtgaac gaagatggct attaatggat gccgccaatg
    73861 cgacttaagc gcagaccagt cggccattcg ccttcttgct tatcgaagct catccagata
    73921 gccgttgccc gaccgaccag attcgcttcc ggcacaaagc cccagtaacg gctgtccgcg
    73981 ctgttgtcgc ggttgtcgcc catcatgaag tattgtcccg gaggaacaat ccaggttgcc
    74041 agttgttgcc ctggctgctg gtaatacatc cccacctgat cctgcgcaat cggcactgtc
    74101 agaatgcggt gcgtcacatc acccagtgtc tctttacgct cggaaagacg aattccattt
    74161 tctttggttt cgtttttcgg cacttcaaag aatccgctgg tcgcttcccc accattacgg
    74221 cgtgagaagg tctgaacgaa atcgctcggt tccacgtttg agtaggtgac cggcagcgcg
    74281 ttttcacacg cctggccgga actgcatccc ggttgaatcg tcagctcttt tgagaccgga
    74341 tcgtaagtga ctttatcgcc cggtaaaccc accgcgcgct tgatgtaatc aagctttgga
    74401 tcttccggat atttaaagac cacgatatcg ccgcgtttcg gatgaccggt ttcgatcagc
    74461 gttttctggt agataggatc tttaatgcca taagcaaact tctctaccag aataaaatca
    74521 ccaattaaca gagtcggcat catcgaacct gacgggatct ggaacggttc ataaataaac
    74581 gaacgcacaa tcaatacgat agccagtacc ggaaaaacag aagcaccggt ttccagccag
    74641 ccaggcttcg gcgcaacctt tttcaacgtt gctttatcca gtgagtcacc ggcagccgcc
    74701 tgcgccgctg cctgacgttc ccgccgttta ggtgcgaaaa agaatttatc cacgcaccat
    74761 aaaatgcccg tcaccagtgt ggcaatcacc agaatcaggg caaacatatt cgccatgcca
    74821 actcctaagg gttatttgtt gtctttgccg acgtgcagaa tggcgaggaa cgcttcctgc
    74881 ggcagctcga cgttaccgat ctgcttcatg cgtttcttac cttctttctg cttctgcagc
    74941 agctttttct tacggctgat atcgccgcca taacatttag ccagtacgtt tttacgcagc
    75001 tgtttcacgg tggatcgcgc aataatgtgc gtaccaatcg ctgcctgaat ggcgatatca
    75061 aactgctggc gtgggatcag atctttcatc ttctccacca actcgcgacc gcggttttgc
    75121 gaattatcac ggtgggtgat cagcgccaac gcatcaacac gttcaccgtt gattaatacg
    75181 tctacacgta ccatgtcgga cgcctggaag cgtttgaagt tgtaatccag agacgcataa
    75241 ccacgcgagg tagatttcag gcgatcgaag aagtcgagca ccacttccgc catcgggatc
    75301 tcgtacgtca gcgccacctg attaccgtgg taaaccatat tggtctgcac gccgcgtttt
    75361 tctacgcaca gcgtaataac gttgccgaga tatgcctgcg gcagcagcat gtgacattcg
    75421 gcaatcggct cgcgcagttc gtagatgtta tttaccgctg gcagcttgga tggactgtcg
    75481 acgtaaataa cttctcttga cgtggtttca acttcataca ctacggtcgg cgcagtggtg
    75541 atcagatcca gatcgtattc acgttccaga cgttcctgga tgatctccat gtgcagcagg
    75601 ccgaggaagc cgcagcggaa accaaagccc agcgcgctgg agctttccgg ctcatagaac
    75661 agtgaagcat cgttcaggct gagtttaccc agcgcgtcac ggaaggcttc atagtcgtcg
    75721 gaacttaccg ggaacagacc ggcgtatacc tgcggtttga ctttcttaaa gccaggcagc
    75781 gccttttctg ccggattacg cgccagcgtt aaggtatcgc cgactggagc gccgtggata
    75841 tctttaatcg cacataccag ccagcctact tcgccacatt tcagttcagt gcggtcaacc
    75901 tgcttcggcg tgaagatgcc cagacggtcg gcgttatagg tctgcccggt actcatgact
    75961 ttcactttgt cgcccttacg cagggtgccg tttttaatac ggataagtga aacaacgccc
    76021 agatagttgt cgaaccagga gtcgataatt agtgcctgca acgggccttc cggatcgcct
    76081 tccggcggcg gaatgtcgcg caccagacgt tcgagaacgt cctgcacgcc aacgccggtt
    76141 ttcgctgaac agcgcaccgc gtcggtggcg tcgatgccga cgatatcttc aatttcttcc
    76201 gccacgcgtt caggatcggc tgccggcagg tcaatcttgt tcagtaccgg caccacttcg
    76261 agatccattt ccatggcggt gtagcagttt gccagggttt gcgcttctac gccctgcccg
    76321 gcgtcgacca ccagcaatgc accttcacag gcagccagcg aacgggaaac ttcataggag
    76381 aagtctacgt ggcccggggt gtcgataaag ttaagctgat aggtttcgcc gtcagacgct
    76441 ttgtagtcca gcgtcacgct ttgcgctttg atggtaatgc cacgctcacg ctcaagatcc
    76501 atggaatcga gaacctgcgc ctccatttca cggtcagaca ggccaccgca gatctggata
    76561 atacggtcag acagcgtcga tttaccgtgg tcaatgtgag ctatgatcga aaagttacgt
    76621 atattcttca taaagtatga ttattgtgcc ttaatgcccg gttaaccagg cttttagaag
    76681 tcgctgttct gagcttaacg tctgtattaa tagaaacgcc gcattctaca ctacaacatt
    76741 gaggcgagga aatgttcata ccgtatggat tgtggtatct ggaaacgtcc tcgcatttgt
    76801 tatgcaaaat gcaataaagc cagtgaaatc actggctcgc gtcttccgaa gatgtttcaa
    76861 atcgcacaag gccaggcggc aatgccacgc ttaagatgat cggttgccat tctgcccggg
    76921 cagcaaactt acgcgagtag ccgcgcgcaa tcaggaaccc gccaatacca ccgaggatcg
    76981 caccacataa tgctgcgacg tcagaagcaa agagtagctg aaatagcgaa gcgatgagga
    77041 ataatcccac cagcggcgac atataaacca gtaatgcgga gctaagcagg ctgccttcgg
    77101 cgatccccaa ttccactttt tgccccggca ctaacggctc atcacagggt acgacaatgg
    77161 tatgcgtggt ttgcgggcca agtttattta acacgcggct accgcaaccg gcgcgtgaag
    77221 cgcagctgct gcatgaagct ttaacatcac aactgaccag cgcctgcccg ttttgccagg
    77281 agacgacggt agcccactct ttgatcattg cgctgccccg aacttaatat tctcggcaat
    77341 gcgtttcgcc gtttgcggcg gcagttcacc gacaatggtg atttcggcgt tatcacgtac
    77401 gcttgtactg acggttctgc gtccggtgcg caacatctga tcggtgctcg atggcgtagc
    77461 gcggttaacg tttaccgaga agctgaataa tccgtcggaa tagagacgtg attcgatagg
    77521 catgttgtcc atcgtcggta gcggacgtcg actactggaa acttcgctaa aaccctgtgg
    77581 caaccaggtt ggcgtccagc tgaatttagc tttttcacct acaggaacag aaagcaacgg
    77641 cggcaaattt gccttcgcca gcgtctgcat actgctgctg atatcctgat tgacgttaaa
    77701 agcaatcacg cgaaattgtt ccagcgtttc accatcgcga tcaaggagat caacccgcat
    77761 cggtaatttc gattcggtgt ccatccacac gatgtagctg tagcgtgtac catctcgggc
    77821 aaccacgcga atgacttcgc aaagacgatc agcaatacgc gtgcgcccga cggagataaa
    77881 gtcgtagtaa ggagaaaggc gtttgaaatc ggtatagata agcgatggca gagaatcaac
    77941 aatgtaatcg ccattaagcg tgaacggttc aagtcccggt tcaaaatagc tgatttcatt
    78001 gccgcgctgt accacttccc ggcgcgggcc atccatttgc aacaattgtg caagagggcg
    78061 gttatcgagg cgtgcatgtc gataacgcag agactcaaca ccctgtttat tgatgctgat
    78121 gaatgacagc tcgtaattca gtgactgact ggccaggttc atctgctgta ataacgcccc
    78181 ggacgcggga gtggccgagg cgtgagcaga gaataacagg ctacctgtca ctaatgacat
    78241 ggcaaaccaa agttgcttca ttactgcgat tgcgttccta aagtttgaat tcctggcacc
    78301 tgtacagcgg cttgctgggt ttgcgcctgc tcaaactgaa gctgttcaga gtggagtcgg
    78361 cgttgcagtt cgtaatcctg caacattgca ttaatgcgac gacgctgctc ctgtacctgc
    78421 tgctgttgac cattgtttgc ggtcgcttca gaaggtactc ccaggcttac cgggctggct
    78481 ttacccatca tcggcagtgt attaaatacc ggcgtttcgg gctgctggga cgtttcagat
    78541 tgtccattat agtgctggac gccaacgata actgcaagcg atacgcatgc tgctacgccc
    78601 atttgggtaa gctgtgccgc ccacggacgt actttctgcc agaatggcat tttctgccat
    78661 tgatgcggcg caggctgggc ttccgggatc aatgtcgccg gttgacgtac tggctcttct
    78721 tcaatggcgg ccatcacgcg tgaagagata tcgaaatgga gcacctcggg agtatcaccc
    78781 cgcattgagt cacggattaa gtgatagctt tcccaggttt tctgcatttc tgggttatga
    78841 gccagttcgt taagcagctc actatccagc gtttcgccat ccattaaagc ggaaagttgt
    78901 tctttctgca tgcctaatac ccttatccag tatcccgcta tcgtcaacgc ctgataagcg
    78961 gttgaacttt gttatcaata gcttccctcg ctcggaagat acgtgaacgc accgtaccta
    79021 ccggacaatc catgatagcg gctatctctt catagctcag gccatccagc tcccgcaagg
    79081 ttattgccat gcgcaaatct tccgggaggg actcaatagt tcggaaaact atctgtctca
    79141 gttcttctga caacattaag ttctcagggt tcgaaatttc tttcaacgcg ccgccacttt
    79201 cgaagttttc agcttcaatg gcatccacat cactggaagg tggacgacgc ccctgagcaa
    79261 ccaggtaatt tttcgctgta tttacagcaa tccgatacag ccatgtataa aaagcgctat
    79321 ctccccggaa cgaatccagc gcacgatagg ctttaataaa agcttcttgt accacatcgg
    79381 gaacatcacc cgacggcaca tagcgggaaa ccagactcgc cactttatgc tgatagcgca
    79441 ctaccagtag gttaaaggct ttctgatctc ccttctggac ccgttcaacc aggacctggt
    79501 ccgttaactg ctcgctcatc cgaggtaaag tctccccaaa ccaaatttcc acgcgctatc
    79561 gaaacgccac tccattagct gcaatttgag caagcaaagg gttagagtgt ctcgtttttg
    79621 taaagttccg taacgcatct gtttttgttt gtcatgctgt agacggatca ttatctatca
    79681 ttataagtct acagaatctg aacatcgcat tatctgtgta gaaatgccca tttaactgcc
    79741 tgaagagtaa cccaacggcc tttttatttc accacctaat cctccaccag ccagtaactt
    79801 ctctttttct cgccgccctg cgtcagcgtg tttagccact gtaacaaata ttaaaatagc
    79861 aggtgtttat tcgcacaaca tgatgctatg ctgaccaaac ggtgtttagt aaattaaaca
    79921 aagaaaatga atactctccc agaacattca tgtgacgtgt tgattatcgg tagcggcgca
    79981 gccggacttt cactggcgct acgcctggct gaccagcatc aggtcatcgt tctaagtaaa
    80041 ggcccggtaa cggaaggttc aacattttat gcccagggcg gtattgccgc cgtgtttgat
    80101 gaaactgaca gcattgactc gcatgtggaa gacacattga ttgccggggc tggtatttgc
    80161 gatcgccatg cagttgaatt tgtcgccagc aatgcacgat cctgtgtgca atggttaatt
    80221 gaccaggggg ttttgtttga tacccacgtt caaccgaatg gcgaagaaag ctaccatctg
    80281 acccgtgaag gtggacatag tcaccgtcgt attcttcatg ccgccgacgc caccggtaga
    80341 gaagtacaat ccacgctggt gagcaaggcg cagaaccatc cgaatattcg tgtgctggag
    80401 cgcagcaacg cggttgatct gattgtctct gacaaaattg gcctgccggg cacgcgacgg
    80461 gttgttggcg cgtgggtatg gaaccgaaat aaagaaacgg tggaaacctg ccacgcaaaa
    80521 gcagtggtac tggcaaccgg cggtgcgtcg aaggtttatc agtacaccac caatccggat
    80581 atttcttctg gcgatggcat tgctatggcg tggcgcgcag gctgtcgggt tgccaatctc
    80641 gaatttaatc agttccaccc taccgcactg tatcacccac aggcacgcaa tttcctgtta
    80701 acggaagcac tgcgcggcga aggcgcttat ctcaagcgcc cggatggcac gcgttttatg
    80761 cccgattttg atgagcgcgg cgaactggcc ccgcgcgata ttgtcgcccg cgccattgac
    80821 catgaaatga aacgcctcgg cgcagattgt atgttcctcg acatcagcca taagcccgcc
    80881 gattttattc gccagcattt cccgatgatt tatgaaaaat tgctcgggct ggggattgat
    80941 ctcacacaag aaccggtacc gattgtgcct gctgcacatt atacctgcgg tggtgtaatg
    81001 gttgatgatc atgggcgtac ggacgtcgag ggcttgtatg ccattggcga ggtgagttat
    81061 accggcttac acggcgctaa ccgcatggcc tcgaattcat tgctggagtg tctggtctat
    81121 ggctggtcgg cggcagaaga tatcaccaga cgtatgcctg atgcccacgg cgtcagtacg
    81181 ttaccgccgt gggatgaaag ccgcgttgag aaccctgacg aacgggtagt aattcagcat
    81241 aactggcacg agctacggct gtttatgtgg gattacgttg gcattgtgcg cacaacgaag
    81301 cgcctggaac gcgccctgcg gcggataacc atgctccaac aagaaataga cgaatattac
    81361 gcccatttcc gcgtctcaaa taatttgctg gagctgcgta atctggtaca ggttgccgag
    81421 ttgattgttc gctgtgcgat gatgcgcaag gagagccgtg gactgcattt tacgctggat
    81481 tacccggaac tgctcaccca ttccggtcca tcgatccttt ctcccggcaa tcattacata
    81541 aacagataaa aagcctgggt cagcgctgta tacgcttcgg aatagttctg gtctggccca
    81601 cgaatgacta agcgatcact aaagcattct cccgcctgcg gggagaatgc cagcagcacc
    81661 cgatgcggca gtcgcgcttc gttttccgcc acatccgtcc gcaaacgtaa atgccagccc
    81721 atgcttaatg ccagttcagt aaaaccatta ccaatctgct ctggcagcac cacgcagaaa
    81781 aatccctctt cggtaatgca ctccgccgca caggtcagca gcgatgggtg atcaagcgta
    81841 gtggtatagc gagcctgttc ccgttgaggt gtcgcacact ctactccctg ctgatagtaa
    81901 ggtgggttac tgatgattaa atcgaagcgc gcggtctgct gtgttaccca ctgctgaata
    81961 tccgccgtat ggacgttaat ccgctctgcc cacggggact ggttgatatt ttcctgcgcc
    82021 tgcgcggcag cttcactttc cagctcaacg gcatcaatta tcacgctgtc actggttcgc
    82081 tgcgccagca tcagtgccag caacccgcta cccgcgccga tatcaaggca acgtttcacc
    82141 ccagccaccg gtgcccatgc gcccagcaaa atgccatccg ttcccacttt catcgcacag
    82201 cgatcgtgag caacaaaaaa ctgtttaaaa gtaaatccat tacgacgaag cacggatgta
    82261 gactgtgaca tgaaaataaa accttgcagg aaaaacggcg atagcaccgg gtgagaacaa
    82321 tacccgagaa gcgatatcca tacaaacaga tgaagattgc agccgtaacg tctataatca
    82381 gcgccccaca cagaggtaga acatgactgt aacgactttt tccgaacttg aactcgacga
    82441 aagcctgctg gaagccctcc aggataaagg tttcactcgc ccgaccgcca ttcaggctgc
    82501 cgccattccg cctgcgctcg atggccgtga tgtactcggt tctgcgccga caggcaccgg
    82561 taaaacggcg gcgtatctgc tgccagcgtt gcagcacctg ctcgatttcc cacgtaagaa
    82621 atccggtcca ccgcgtattt tgatcctcac cccaactcgc gagctggcga tgcaggtggc
    82681 cgatcatgcc cgcgaactgg cgaaacatac gcatctggat atcgccacca tcaccggcgg
    82741 cgtagcctat atgaaccacg cggaagtgtt cagcgaaaat caggacatcg tggtcgccac
    82801 gaccggacgt ctgctgcaat acataaaaga agagaacttc gattgccgcg cggttgaaac
    82861 gctgatcctc gacgaagcag accgtatgct ggatatgggc ttcgctcagg atatcgaaca
    82921 tattgctggc gaaacgcgct ggcgtaaaca gaccctgctc ttttcggcaa cgctggaagg
    82981 cgatgcgatt caggactttg ccgagcgtct gctggaagat ccggtggagg tttctgccaa
    83041 tccgtccacc cgtgagcgca aaaaaattca tcagtggtat taccgcgccg atgacctgga
    83101 acataaaacc gcattgctgg tgcatctgtt aaaacagccg gaagcgaccc gctcaattgt
    83161 gtttgtgcgt aagcgtgaac gtgtgcatga gctggcaaac tggctgcgcg aagcgggcat
    83221 caacaactgc tatctcgaag gtgagatggt acaaggcaag cgtaacgaag cgatcaagcg
    83281 tttgaccgaa ggtcgcgtaa acgtactggt tgcaaccgat gttgccgcgc gcggtatcga
    83341 cattcctgac gtcagccacg tctttaactt cgatatgccg cgcagtggcg atacttattt
    83401 gcaccgtatc ggacgtaccg cgcgcgccgg tcgtaaaggc accgcaattt cgctggtgga
    83461 agcccatgac catttgctgc tgggcaaggt tggccgttac attgaagagc caattaaagc
    83521 tcgcgttatt gatgagttac gcccgaaaac gcgtgcgcca agcgaaaagc agaccggcaa
    83581 gccatcgaag aaagtactgg ctaaacgtgc cgagaagaaa aaagctaaag agaaagagaa
    83641 gccgcgggtg aaaaaacgcc atcgcgacac caaaaatatt ggtaagcgcc gtaaaccaag
    83701 cggaacgggc gtgccaccgc aaacgacaga agagtaatct caatgccggg tttaagcctg
    83761 gtattaaaag tgctggcaaa cgcaaaactg cctgatgcgc tacgcttatc aggcctacgt
    83821 ggctcatgca atatattgaa tttgcacgat cttgtaggcc ggataaggcg ttcacgccgc
    83881 atccggcatg aataaagcgc actttgtcga caagttaaat gccgggttta atcccgacat
    83941 tatttttatc cagccacaaa actctcttca acacactgaa taaaagtgtg catcgccggg
    84001 ctaaccgctt ttccagcatg gtgtgcgcac atggccgtaa tggtctgcga ctgttcgcca
    84061 aaaggcaatt caattaattc tccgctctca agctcttttt ccaccgcaaa gcggggcaag
    84121 taactgacac cgatattcgc cgcgacacaa cgtttgatac tttcaatgct taacagctca
    84181 atggtgtttt ccaccgtgat ccgccgctga cgcagcgtgc tctcaaatat ctgccggaag
    84241 acacattgcg gttcgttgat aataaagcta caggcgttat gtcttcccgg ctcagtaaaa
    84301 tcgacatctg caatttgcgg tgaagccacc agcaccagcg attgttcacc caactctcgt
    84361 cggttcaggg catcatcatt ccctacgcga taaaagacgc cgacatcggc ctcatcattc
    84421 agtagcgcat cacggatcac gtaacagttc agcgactgca acgataaacg cacttttggt
    84481 gcgcgttgcc gaaaacgctg caacacctgc ggcattcggt aagagagcaa cgtttcgccc
    84541 gaaacaacgc gtagttcccc gtccggatct gactcctttt tagcagcctc acgaagcgta
    84601 tccatcactc gggtaagttc ataaatgtgc ggcagcaact tttttccttc gcgggtaagg
    84661 cacatccgtc ggccaatttt ctcaaataac tggactgaaa actcctgctc aagctgctga
    84721 atatgaaaag tcaccgtcga ttgtgtacag cacaattttt gcgaagctcg caaaaaggaa
    84781 ccctcttcca ccacggtttt aagcgtaata aaacggcgca gatccataac cccaaaccta
    84841 tcgaaaatat cgaatctaga atataaaaac attcattttt ttaaatgttc cgtgtcgggt
    84901 actgtctacc aaaacagagg agataacaag tgacaccgac ccttttaagt gctttttgga
    84961 cttacaccct gattaccgct atgacgccag gaccgaacaa tattctcgcc cttagctctg
    85021 ctacgtcgca tggatttcgt caaagtaccc gcgtgctggc agggatgagt ctgggatttt
    85081 tgattgtgat gttactgtgt gcgggcattt cattttcact ggcagtgatt gacccggcag
    85141 cggtacacct tttgagttgg gcgggggcgg catatattgt ctggctggcg tggaaaatcg
    85201 ccaccagccc aacaaaggaa gacggacttc aggcaaaacc aatcagcttt tgggccagct
    85261 ttgctttgca gtttgtgaac gtcaaaatca ttttgtacgg tgttacggca ctgtcgacgt
    85321 ttgttctgcc gcaaacacag gcgctaagct gggtagttgg cgtcagcgtt ttgctggcga
    85381 tgattgggac gtttggcaat gtatgctggg cgctggcggg gcatctgttt cagcaattgt
    85441 ttcgccagta tggtcgccag ttaaatatcg tgcttgccct gttgctggtc tattgcgcgg
    85501 tacgcatttt ctattaacga aaaaaagcgg aagaggtcgc cctcttccgc ttagtaactt
    85561 gctacttaag ccttacaggc tttcagtaaa ggtacgagcg ataacgtcgc gctgctgttc
    85621 cggagtcaga gagttaaagc gaactgcata accggataca cggatggtca gctgcggata
    85681 tttttccgga tgcttaactg catcttccag agtttcacga cgcagaacgt taacgttcag
    85741 gtgttgacca ccttcaacgc gaacttctgg tttcacttct actggaactt cacggtattc
    85801 aatgtcaccc agtttgctta ctgcaaccac ttcatcttct gcataacctg cttttgcaac
    85861 gatgcaacgt gcttcgcctt tttcgctgtc cagcagccag aaagagttca gcagatcgtc
    85921 gttagcggct ttagtaatct ggatacctgt aatcatgtga tgcctccccg gcaaaattat
    85981 ttgatttgtt cagcctgtcg cggccaattg gtaaaaccat tgttgcttga gtgtatatat
    86041 actcctcaac cacccttgat tctttgattt aaatcaataa aaaccacaca tcgattgtgg
    86101 tcgtaaatgg attttattgt tttacatcaa cttatgcggg tgtgaaattt taccaattta
    86161 catttttttg cactcgttta agtctaaaaa atgagcatga ttttgttctg tagaaagaag
    86221 cagttaagct aggcggattg aagattcgca ggagagcgag atggctaacg aattaacctg
    86281 gcatgacgtg ctggctgaag agaagcaaca accttatttt cttaataccc ttcagaccgt
    86341 cgccagcgag cggcagtccg gcgtcactat ctatccacca caaaaagatg tctttaacgc
    86401 gttccgcttt accgagttgg gtgacgttaa agtggtgatt ctcggccagg atccttatca
    86461 cggaccggga caggcgcatg gtctggcatt ttccgttcgt cccggcattg ccattcctcc
    86521 gtcattattg aatatgtata aagagctgga aaatactatt ccgggcttca cccgccctaa
    86581 tcatggctat ctcgaaagct gggcgcgtca gggcgttctg ctgctcaata ctgtgttgac
    86641 ggttcgcgca ggtcaggcgc attcccacgc cagcctcggt tgggagacgt ttactgataa
    86701 agtcatcagc ctgattaacc agcaccgcga aggcgtggtg tttttgttgt ggggatcaca
    86761 tgcgcaaaag aaaggggcga ttatagataa gcaacgccat catgtactga aagcaccgca
    86821 tccgtcgccg ctttcggcgc atcgtggatt ctttggctgc aaccattttg tgctggcaaa
    86881 tcagtggctg gaacaacgtg gcgagacgcc gattgactgg atgccagtat taccggcaga
    86941 gagtgagtaa atttgcggta aaatgccgga tggcagagtt gccacccggc tgatttatca
    87001 ggctttattc tgacgccacc attcaccaag caaaacgccg gttgcaacag agatattcag
    87061 cccggcaacg ttgcccgtac cgtcaatctt cacgcgcaga tcgttcggat cgcgtgcggc
    87121 atccggtaac ccttcatatt cctgacccag caccagtacc attttcgctg gcagactggt
    87181 tttgaacagc ggtttcccct gctcgctgga agtggtcact acggtgtaac ctgcctgacg
    87241 gaaatcatcc aacacgttaa caatgttgtc gccagtaatc ggctgaacgt gctctgcgcc
    87301 gccttctgcg gtacggatag ccgcccccga ctccagcagt gccgcatcct gcaccacaac
    87361 gcctttcacg ccgaagtgcg cgcagctgcg catcatgcca cccaggttat gcgggttaga
    87421 ttcgttttcc agcgccagta cacaatcctg cgcgcctgcc tggcttaccc actgctgcac
    87481 ggttgtgcca ttacgctttt tgatcaggaa gcaaacgccg ccgtgatgtt ccgtgcctga
    87541 cgcttttgtc aattccgctt catccaccac atggtacgct ttgcggtttg ctgccatcca
    87601 gcgcaaggct tctttaaaac gcggcgttac actctggata aaccaggcgc gaacaatcgc
    87661 ttccggacgg ctctggaaaa gtgcctgaca tgcattttcg ccgtagacgc gggtttcttc
    87721 cgcacgctga cgacgcaaca cttccggatc aataaaactt ttaccactga tgccaccgtg
    87781 atcggccttt tccggcgtct catcacccgg cgcgcgggaa accgtgcgcc acggcgaatc
    87841 ttcccacttg cggtcacggg gctgactctg tttgtcatcg cgggcggggc gacggccacc
    87901 gtcagcacga gattttcctg gacgcccgcc ccctttcccg gtacgcgggt tgtgggtacg
    87961 tttatcagaa tcatcatcac tgcggacata catcactttg accttgccgc ttttaccttt
    88021 catttcatcg ttcatgcttt tctccaccag cgctgcgcga agcgcgcaga ttacccgaag
    88081 tccgcgcggt tcgccatgat ttcgtaccaa agcctgcgac tatcatacct attgaataaa
    88141 acagattgtt gtctggaaca atgtccccga taatatgtaa catattagaa acataccggc
    88201 gtcgttgccg ataagtctcc ttactcatcc cgaggttagt tatgaatacc gtttgtaccc
    88261 attgtcaggc catcaatcgc attcccgacg atcggatcga agatgcggca aaatgcggac
    88321 gctgcggtca cgacttgttt gacggagagg tgattaatgc gaccggtgaa acactcgaca
    88381 aattgctgaa ggatgatcta cctgtggtga tcgacttctg ggcaccgtgg tgcggcccct
    88441 gccgtaattt cgcaccaatt tttgaagatg tcgcgcaaga gcgtagcggt aaagtgcgct
    88501 ttgtgaaagt gaataccgaa gctgaacgtg aattgagcag tcgctttgga attcgtagta
    88561 taccgacgat catgattttc aaaaacggtc aggttgtcga catgcttaat ggcgcagtac
    88621 cgaaagcgcc gttcgatagc tggctgaacg aatctcttta atcttaccgg ggcgcatctt
    88681 gtgccccgtt ttctcctctg cgacaatggc gttttttcga cgctctctta tgaccgaaaa
    88741 cgctgttctc cagttacgcg ccgagcgtat tgcgcgcgca acacgtcctt ttcttgcccg
    88801 cggtaatcgc gttcgtcgct gccaacgctg tcttttgcca gagaaattat gtctctgttc
    88861 gacaattacc ccagcacaag caaaaagtcg cttctgtttg ctgatgttcg acaccgagcc
    88921 aatgaagccc agtaataccg ggcgtctgat tgctgatatt ttgcctgata ccgttgcgtt
    88981 tcaatggtcg cgtaccgaac cctcgcaaga tttactggat ctggtacaaa acccggacta
    89041 tcagccaatg gtggtctttc ccgcttcgta tgctgatgag caacgggaag tgatcttcac
    89101 accacctgcc ggtaagccac cgctgtttat catgctcgat ggtacctggc cggaagctcg
    89161 caagatgttt cgtaaaagtc cgtatctgga taatcttccc gtcatttccg tcgatctttc
    89221 ccggctttct gcctatcgcc tgcgtgaagc ccaggctgaa ggccaatatt gtactgccga
    89281 ggtagccatc gcactgttag atatggccgg cgataccggg gcggcagcag ggttaggcga
    89341 gcattttacc cgctttaaaa cacgctatct ggcaggaaaa acgcaacatc tgggtagcat
    89401 cacagcagaa cagttagaaa gcgtttaaaa tcattcggtc acttctgcgg gagaccggta
    89461 tgagtcagcg aggactggaa gcactactgc gaccaaaatc gatagcggta attggcgcgt
    89521 cgatgaaacc caatcgcgca ggttacctga tgatgcgtaa cctgctggcg ggaggcttta
    89581 acggaccggt actcccggtg acgccagcct ggaaagcggt gttgggtgtg ttggcctggc
    89641 cggatattgc cagcttgccc tttacacccg accttgcggt tttatgtacc aatgccagcc
    89701 gtaatcttgc tcttctggaa gagctcggcg agaaaggctg taaaacctgc attattcttt
    89761 ccgccccggc atcgcaacac gaagatctcc gcgcctgcgc cctgcgccat aacatgcgcc
    89821 tgcttggacc aaacagtctg ggtttactgg ctccctggca aggtctgaat gccagctttt
    89881 cgcctgtgcc gattaaacgc ggcaagctgg cgtttatttc acaatcggca gccgtctcca
    89941 acaccatcct cgactgggcg caacagcgtg agatgggctt ttcctacttt attgcgctcg
    90001 gcgacagcct ggatatcgac gttgatgaat tgctcgacta tctggcgcgc gacagtaaaa
    90061 ccagcgccat cctgctctat ctcgaacagt taagcgacgc gcgacgcttt gtttcggcag
    90121 cccgtagtgc ctcgcgtaat aaaccgattc tggtgattaa aagcggacgt agcccggcgg
    90181 cacagcgact gctcaacacg acggcaggaa tggacccggc atgggatgcg gctattcagc
    90241 gtgccggttt gttgcgggta caggataccc acgaactgtt ttcggcggtg gaaaccctta
    90301 gccatatgcg cccgctgcgt ggcgaccggt tgatgattat cagcaacggt gctgcgcctg
    90361 ccgcgctggc gctggatgcc ttatggtcac gcaatggcaa gctggcaacg ctaagcgaag
    90421 aaacctgcca gaaactgcgc gatgcactgc caggacatgt ggcaatctct aacccgctcg
    90481 atctgcgcga tgacgccagc agtgagcact atattaaaac gctggacatt ctgctccaca
    90541 gccaggattt tgatgcgctg atggttattc attcgcccag cgccgctgct cccgcaacag
    90601 aaagcgcgca agtattaatt gaagcggtaa agcatcatcc ccgcagcaag tatgtttctc
    90661 tgctgacgaa ctggtgtggc gaacattcct cgcaagaggc acgacgatta ttcagcgaag
    90721 ccgggctgcc gacctaccgt accccggaag gaaccatcac tgcttttatg catatggtgg
    90781 agtaccggcg taatcagaag caactacgcg aaacgccggc gttgcccagc aatctgacct
    90841 ccaataccgc agaagcgcat cttctgttgc aacaggcgat tgccgaagga gcaacgtcac
    90901 tcgataccca tgaagttcag cctatcctgc aagcatatgg catgaacacg ctccccacct
    90961 ggattgccag cgatagcacc gaagcggtgc atattgccga acagattggt tatccggtgg
    91021 cgctgaaatt gcgttcgccg gatattccac ataaatcgga agttcagggc gtcatgctct
    91081 acctgcgtac agccaatgaa gtccagcaag cggcaaacgc cattttcgat cgcgtaaaaa
    91141 tggcctggcc gcaggcacgg gtccacggcc tgttggtgca aagtatggct aaccgtgctg
    91201 gtgctcagga gttgcgggtt gtggttgagc acgatccggt tttcgggccg ttgatcatgc
    91261 tgggcgaagg cggtgtggag tggcgtcctg aagatcaagc cgtcgtcgcg ttaccgccgc
    91321 tgaacatgaa cctggcccgc tatctggtta ttcaggggat caaaagtaaa aagattcgtg
    91381 cacgcagtgc gctacgccca ttggatgttg caggcttgag ccagcttctg gtgcaggttt
    91441 ccaacttgat tgtcgattgc ccggaaattc agcgtctgga tattcatcct ttgctggctt
    91501 ctggcagtga atttaccgcg ctggatgtca cgctggatat ctcgccgttt gaaggcgata
    91561 acgagagtcg gctggcagtg cgcccttatc cgcatcagct ggaagaatgg gtagaattga
    91621 aaaacggtga acgctgcttg ttccgcccga ttttgccaga agatgagcca caacttcagc
    91681 aattcatttc gcgagtcacc aaagaagatc tttattaccg ctactttagc gagatcaacg
    91741 aatttaccca tgaagattta gccaacatga cacagatcga ctacgatcgg gaaatggcgt
    91801 ttgtagcggt acgacgtatt gatcaaacgg aagagatcct cggcgtcacg cgtgcgattt
    91861 ccgatcctga taacatcgat gccgaatttg ctgtgctggt tcgctcggat ctcaaagggt
    91921 taggcttagg tcgacgctta atggaaaagt tgattaccta tacgcgagat cacggactac
    91981 aacgtctgaa tggtattacg atgccaaaca atcgtggcat ggtggcgcta gcccgcaagc
    92041 tcgggtttaa cgttgatatc cagctcgaag aggggatcgt tgggcttacg ctaaatcttg
    92101 cccaacgcga ggaatcatga gtaaggtact ggaaatgttg accacttaat cgggactggt
    92161 gttattattg cccgcttatg tcgtctgcat tgcacagagg acccttcaat gaacagagaa
    92221 gaaatgcact gtgatgttgt caaaatttaa gcgtaataaa catcaacaac accttgccca
    92281 actacccaag atttctcaat cagttgatga tgtcgatttc ttttacgctc ccgccgactt
    92341 ccgggagacg ctgctggaaa aaatagccag cgcgaagcag cgcatttgca ttgtcgccct
    92401 gtatctcgaa caggatgacg gtggcaaagg cattctgaac gcgttgtatg aagctaaaag
    92461 gcagcgtccg gaactggatg tgcgggtgct ggtcgactgg catcgtgcac aacgtggacg
    92521 cattggcgct gcggcatcta acactaacgc tgactggtac tgccgcatgg cgcaggaaaa
    92581 tccgggcgta gatgttccgg tttatggcgt tccaatcaat acccgtgaag cccttggtgt
    92641 tctgcacttt aaaggcttta tcatcgacga tagcgtactt tatagcggtg ccagcctgaa
    92701 cgatgtttac ctgcatcagc acgataaata tcgctacgac cgttatcatc ttatccgtaa
    92761 ccgtaagatg tcagacatta tgtttgaatg ggttacacag aatattatga atggccgcgg
    92821 cgttaatcgt ctggatgatg ttaatcggcc aaaaagcccg gaaatcaaga acgatattcg
    92881 tctgttccgc caggagctgc gtgatgccgc ttatcatttc cagggcgatg ccgacaacga
    92941 tcagctttcc gtaacgccgc tagtggggct ggggaaatcg agtctgttga acaagaccat
    93001 tttccatctt atgccttgtg ccgagcagaa actaaccatc tgtacgccat acttcaacct
    93061 gccagcaatc cttgtgcgca atattatcca gttgctgcgc gaagggaaaa aggtcgaaat
    93121 tattgttggt gataaaaccg cgaacgactt ctacattccg gaagatgaac ctttcaagat
    93181 aattggcgca ttgccttatc tctatgagat caatctgcgt cgtttcctga gccgtttgca
    93241 gtattacgtc aatactgacc agctagtggt tcggttatgg aaagatgacg acaacaccta
    93301 tcacctaaaa gggatgtggg ttgatgataa gtggatgttg atcaccggta ataacctgaa
    93361 cccgcgcgcc tggcgtctgg atctggaaaa cgccattttg atccacgatc cacaactgga
    93421 gcttgcgccg cagcgagaga aagaactgga gctgatccgc gagcatacca ccatcgttaa
    93481 gcactatcgc gatctgcaaa gtattgccga ttatccggtg aaggttcgta aactcatccg
    93541 ccgtttgcgc cgtatccgca tcgaccgatt aattagccgc atcctgtaat cacaaccccg
    93601 tcctgtacgg ggtttgtttt ttggaggcca cgttttgcgt attctttttg tctgttcgct
    93661 attgttactt tctggatgca gccatatggc taacgatagc tggagcgggc aggataaagc
    93721 tcaacacttt atcgcctcgg cgatgctttc cgccgccgga aatgaatatt cacagcatca
    93781 ggggatgagc cgggatcgca gtgccatgtt tggattgatg ttctctgtca gtttgggggc
    93841 gtcaaaagag ctttgggata gccgccccga agggagcggc tggagctgga aggatttggc
    93901 ctgggatgtc gccggtgcaa gcaccggcta taccgtctgg caactgaccc gtcactaaag
    93961 acgcatcccc ttccctttgc gatgtagcat caaagaaacc agaaacgcca ccacggccat
    94021 caaggtcaca taccagaaga aggctgtttc cattcctatt gatttcagcg acaacgctac
    94081 gtactccgcc gaaccaccaa atatagcatt agcgaccgca tatgacaggc caacgcctaa
    94141 tgcgcgaacc tgtgccggga acatctcagc cttcagtatt ccactgattg atgtataaaa
    94201 actcactatc agcagggcac acatcaccag accaaaagcg gcataaggcg aggaaacgtt
    94261 ttgcaatgct gagagaatag gaacggtaaa aatggctgcc agcgaaccga aacataacat
    94321 tgaggtacgg cgaccaatct tatccgacag cgcgccaatg agtggttgaa taagcatgaa
    94381 tacaaacaat gcggcagtca taatgccact cgccacgttg gcatgcattc ccgcagtatt
    94441 taccagatac ttctgcatat aagtggtgaa ggtatagaaa caaagggagc ccgcagcggt
    94501 aaaaccgaga accatgatga atgcacggcg attgcgccat aatcctttca gagatccagc
    94561 ttcttttaaa gcgcgcgttt cttgttgcga agtttcatct aattgacgac gtaaccacaa
    94621 cgccacaaca gctaacacag ctcctaacgc gaaaggaata cgccatcccc actctctgag
    94681 tgcagcgtct tccatggtgt gttgtaaaac cacgacaacc agtagggcta gcagttgtcc
    94741 gccgatcaac gtcacatact gaaatgatgc gtaaaaacct ttgcgccctt caacggcaac
    94801 ttcactcata taggtggcgc tggtgccata ttctccgcca acggataatc cctgaaataa
    94861 acgagcgaga agcaataatg ccggagccca cgtacctata gtttcataac ctgggaggca
    94921 ggcgataacc agcgatccga aacacatcat acacaccgat aacagcatcg attttttgcg
    94981 accatgttta tcggctatgc ggccaaatag ccaaccgcct attgggcgca tcaggaatcc
    95041 cgcagcaaaa acacctgctg tttgtagtag ttgagtcgtc gtgttcccgg aagggaagaa
    95101 aatgtgggcg aagtagagtg aacagaacga gtagacatag aaatcgaacc actcgaccag
    95161 attacctgaa gaggccccca caatcgccca aatgcggcga cgagtatcac tacttgtcag
    95221 tttgctgtct gccgttacag tactttcagc catgccatta tgtctcctgc cgtaatccga
    95281 tgcttttgtc ggtcgctttt atttatttct tttggtaaag aaaatgttat acatttgttg
    95341 cgtatcatta tgcaacctta accatgaatt tagttagcag gaaaatggtt attgaggagc
    95401 ttaaggataa atttctggta aggaggacac gtatggaagt aggcaagttg gggaaaccgc
    95461 atccgttgct gaatctggca tatgtgggag tataagacgc gcagcgtcgc atcaggcatt
    95521 tttttctgcg ccaatgcaaa aaggccatcc tgacggatgg ccttt
//
LOCUS       DAJOWB010000004        91167 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJOWB010000004 DAJOWB010000000
VERSION     DAJOWB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 91167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 91167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..91167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.004"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            88..672
                     /locus_tag="OHO85_000297"
     CDS             88..672
                     /locus_tag="OHO85_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2669730.1"
                     /translation="MNKVTKTAIAGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSAGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            752..1447
                     /locus_tag="OHO85_000298"
     CDS             752..1447
                     /locus_tag="OHO85_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCQ2669731.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDNVNANELTLVFKTRIKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1477..3993
                     /locus_tag="OHO85_000299"
     CDS             1477..3993
                     /locus_tag="OHO85_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCQ2669732.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYSGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSVNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            4004..5095
                     /locus_tag="OHO85_000300"
     CDS             4004..5095
                     /locus_tag="OHO85_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2669733.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLKANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5138..5998)
                     /gene="rsmI"
                     /locus_tag="OHO85_000301"
     CDS             complement(5138..5998)
                     /gene="rsmI"
                     /locus_tag="OHO85_000301"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCQ2669734.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            6063..8099
                     /gene="lpoA"
                     /locus_tag="OHO85_000302"
     CDS             6063..8099
                     /gene="lpoA"
                     /locus_tag="OHO85_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCQ2669735.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            8057..8452
                     /locus_tag="OHO85_000303"
     CDS             8057..8452
                     /locus_tag="OHO85_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCQ2669736.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8472..9062
                     /gene="diaA"
                     /locus_tag="OHO85_000304"
     CDS             8472..9062
                     /gene="diaA"
                     /locus_tag="OHO85_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCQ2669737.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            9072..9647
                     /gene="yraP"
                     /locus_tag="OHO85_000305"
     CDS             9072..9647
                     /gene="yraP"
                     /locus_tag="OHO85_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCQ2669738.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9852..10892)
                     /locus_tag="OHO85_000306"
     CDS             complement(9852..10892)
                     /locus_tag="OHO85_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCQ2669739.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWEPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGTVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10965..11600)
                     /locus_tag="OHO85_000307"
     CDS             complement(10965..11600)
                     /locus_tag="OHO85_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCQ2669740.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAESMRPEHEGVTILSSSELRKRAE"
     gene            11728..12246
                     /gene="yhbO"
                     /locus_tag="OHO85_000308"
     CDS             11728..12246
                     /gene="yhbO"
                     /locus_tag="OHO85_000308"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCQ2669741.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12226..12669)
                     /locus_tag="OHO85_000309"
     CDS             complement(12226..12669)
                     /locus_tag="OHO85_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCQ2669742.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            12720..13022
                     /gene="yhbQ"
                     /locus_tag="OHO85_000310"
     CDS             12720..13022
                     /gene="yhbQ"
                     /locus_tag="OHO85_000310"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCQ2669743.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(13009..13512)
                     /locus_tag="OHO85_000311"
     CDS             complement(13009..13512)
                     /locus_tag="OHO85_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCQ2669744.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13506..14030)
                     /locus_tag="OHO85_000312"
     CDS             complement(13506..14030)
                     /locus_tag="OHO85_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCQ2669745.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14239..15234
                     /locus_tag="OHO85_000313"
     CDS             14239..15234
                     /locus_tag="OHO85_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCQ2669746.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15243..16121
                     /locus_tag="OHO85_000314"
     CDS             15243..16121
                     /locus_tag="OHO85_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCQ2669747.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16327..17334
                     /locus_tag="OHO85_000315"
     CDS             16327..17334
                     /locus_tag="OHO85_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCQ2669748.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPNVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17452..18696)
                     /gene="mtr"
                     /locus_tag="OHO85_000316"
     CDS             complement(17452..18696)
                     /gene="mtr"
                     /locus_tag="OHO85_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCQ2669749.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18850..20739)
                     /gene="deaD"
                     /locus_tag="OHO85_000317"
     CDS             complement(18850..20739)
                     /gene="deaD"
                     /locus_tag="OHO85_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCQ2669750.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20732..20812)
                     /gene="yrbN"
                     /locus_tag="OHO85_000318"
     CDS             complement(20732..20812)
                     /gene="yrbN"
                     /locus_tag="OHO85_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCQ2669751.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20919..21803)
                     /gene="nlpI"
                     /locus_tag="OHO85_000319"
     CDS             complement(20919..21803)
                     /gene="nlpI"
                     /locus_tag="OHO85_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCQ2669752.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21912..24047)
                     /gene="pnp"
                     /locus_tag="OHO85_000320"
     CDS             complement(21912..24047)
                     /gene="pnp"
                     /locus_tag="OHO85_000320"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCQ2669753.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24294..24563)
                     /gene="rpsO"
                     /locus_tag="OHO85_000321"
     CDS             complement(24294..24563)
                     /gene="rpsO"
                     /locus_tag="OHO85_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCQ2669754.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24712..25656)
                     /gene="truB"
                     /locus_tag="OHO85_000322"
     CDS             complement(24712..25656)
                     /gene="truB"
                     /locus_tag="OHO85_000322"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCQ2669755.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25656..26057)
                     /gene="rbfA"
                     /locus_tag="OHO85_000323"
     CDS             complement(25656..26057)
                     /gene="rbfA"
                     /locus_tag="OHO85_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCQ2669756.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALIMQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(26221..28893)
                     /gene="infB"
                     /locus_tag="OHO85_000324"
     CDS             complement(26221..28893)
                     /gene="infB"
                     /locus_tag="OHO85_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCQ2669757.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28918..30405)
                     /gene="nusA"
                     /locus_tag="OHO85_000325"
     CDS             complement(28918..30405)
                     /gene="nusA"
                     /locus_tag="OHO85_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCQ2669758.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30433..30885)
                     /gene="rimP"
                     /locus_tag="OHO85_000326"
     CDS             complement(30433..30885)
                     /gene="rimP"
                     /locus_tag="OHO85_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCQ2669759.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(31092..31168)
                     /locus_tag="OHO85_000327"
     tRNA            complement(31092..31168)
                     /locus_tag="OHO85_000327"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31132..31134),aa:Met,seq:cat)
     gene            31516..32859
                     /gene="argG"
                     /locus_tag="OHO85_000328"
     CDS             31516..32859
                     /gene="argG"
                     /locus_tag="OHO85_000328"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCQ2669760.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(32867..34492)
                     /locus_tag="OHO85_000329"
     CDS             complement(32867..34492)
                     /locus_tag="OHO85_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCQ2669761.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVILFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34951..35037)
                     /locus_tag="OHO85_000330"
     tRNA            complement(34951..35037)
                     /locus_tag="OHO85_000330"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35001..35003),aa:Leu,seq:gag)
     gene            complement(35052..35384)
                     /gene="secG"
                     /locus_tag="OHO85_000331"
     CDS             complement(35052..35384)
                     /gene="secG"
                     /locus_tag="OHO85_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCQ2669762.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35612..36949)
                     /gene="glmM"
                     /locus_tag="OHO85_000332"
     CDS             complement(35612..36949)
                     /gene="glmM"
                     /locus_tag="OHO85_000332"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCQ2669763.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36954..37790)
                     /gene="folP"
                     /locus_tag="OHO85_000333"
     CDS             complement(36954..37790)
                     /gene="folP"
                     /locus_tag="OHO85_000333"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCQ2669764.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     EN"
     gene            complement(37880..39814)
                     /gene="ftsH"
                     /locus_tag="OHO85_000334"
     CDS             complement(37880..39814)
                     /gene="ftsH"
                     /locus_tag="OHO85_000334"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCQ2669765.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39914..40543)
                     /gene="rlmE"
                     /locus_tag="OHO85_000335"
     CDS             complement(39914..40543)
                     /gene="rlmE"
                     /locus_tag="OHO85_000335"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCQ2669766.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40669..40962
                     /gene="yhbY"
                     /locus_tag="OHO85_000336"
     CDS             40669..40962
                     /gene="yhbY"
                     /locus_tag="OHO85_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCQ2669767.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(41118..41594)
                     /gene="greA"
                     /locus_tag="OHO85_000337"
     CDS             complement(41118..41594)
                     /gene="greA"
                     /locus_tag="OHO85_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCQ2669768.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41842..43275
                     /gene="dacB"
                     /locus_tag="OHO85_000338"
     CDS             41842..43275
                     /gene="dacB"
                     /locus_tag="OHO85_000338"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCQ2669769.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(43461..44633)
                     /gene="cgtA"
                     /locus_tag="OHO85_000339"
     CDS             complement(43461..44633)
                     /gene="cgtA"
                     /locus_tag="OHO85_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCQ2669770.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(44649..45614)
                     /locus_tag="OHO85_000340"
     CDS             complement(44649..45614)
                     /locus_tag="OHO85_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCQ2669771.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(45741..45998)
                     /gene="rpmA"
                     /locus_tag="OHO85_000341"
     CDS             complement(45741..45998)
                     /gene="rpmA"
                     /locus_tag="OHO85_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCQ2669772.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(46019..46330)
                     /gene="rplU"
                     /locus_tag="OHO85_000342"
     CDS             complement(46019..46330)
                     /gene="rplU"
                     /locus_tag="OHO85_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCQ2669773.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            46589..47560
                     /gene="ispB"
                     /locus_tag="OHO85_000343"
     CDS             46589..47560
                     /gene="ispB"
                     /locus_tag="OHO85_000343"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCQ2669774.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            47787..48065
                     /gene="sfsB"
                     /locus_tag="OHO85_000344"
     CDS             47787..48065
                     /gene="sfsB"
                     /locus_tag="OHO85_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCQ2669775.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(48113..49372)
                     /gene="murA"
                     /locus_tag="OHO85_000345"
     CDS             complement(48113..49372)
                     /gene="murA"
                     /locus_tag="OHO85_000345"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCQ2669776.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICRGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(49427..49681)
                     /gene="ibaG"
                     /locus_tag="OHO85_000346"
     CDS             complement(49427..49681)
                     /gene="ibaG"
                     /locus_tag="OHO85_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCQ2669777.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49841..50134)
                     /gene="mlaB"
                     /locus_tag="OHO85_000347"
     CDS             complement(49841..50134)
                     /gene="mlaB"
                     /locus_tag="OHO85_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCQ2669778.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(50134..50769)
                     /gene="mlaC"
                     /locus_tag="OHO85_000348"
     CDS             complement(50134..50769)
                     /gene="mlaC"
                     /locus_tag="OHO85_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCQ2669779.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50788..51339)
                     /gene="mlaD"
                     /locus_tag="OHO85_000349"
     CDS             complement(50788..51339)
                     /gene="mlaD"
                     /locus_tag="OHO85_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCQ2669780.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(51344..52126)
                     /gene="mlaE"
                     /locus_tag="OHO85_000350"
     CDS             complement(51344..52126)
                     /gene="mlaE"
                     /locus_tag="OHO85_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCQ2669781.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(52134..52943)
                     /gene="mlaF"
                     /locus_tag="OHO85_000351"
     CDS             complement(52134..52943)
                     /gene="mlaF"
                     /locus_tag="OHO85_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCQ2669782.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            53153..54130
                     /locus_tag="OHO85_000352"
     CDS             53153..54130
                     /locus_tag="OHO85_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCQ2669783.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            54144..55130
                     /gene="kdsD"
                     /locus_tag="OHO85_000353"
     CDS             54144..55130
                     /gene="kdsD"
                     /locus_tag="OHO85_000353"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCQ2669784.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            55151..55717
                     /gene="kdsC"
                     /locus_tag="OHO85_000354"
     CDS             55151..55717
                     /gene="kdsC"
                     /locus_tag="OHO85_000354"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCQ2669785.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            55714..56289
                     /gene="lptC"
                     /locus_tag="OHO85_000355"
     CDS             55714..56289
                     /gene="lptC"
                     /locus_tag="OHO85_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCQ2669786.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            56258..56815
                     /gene="lptA"
                     /locus_tag="OHO85_000356"
     CDS             56258..56815
                     /gene="lptA"
                     /locus_tag="OHO85_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCQ2669787.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56822..57547
                     /gene="lptB"
                     /locus_tag="OHO85_000357"
     CDS             56822..57547
                     /gene="lptB"
                     /locus_tag="OHO85_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCQ2669788.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            57595..59028
                     /gene="rpoN"
                     /locus_tag="OHO85_000358"
     CDS             57595..59028
                     /gene="rpoN"
                     /locus_tag="OHO85_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCQ2669789.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            59051..59338
                     /gene="hpf"
                     /locus_tag="OHO85_000359"
     CDS             59051..59338
                     /gene="hpf"
                     /locus_tag="OHO85_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCQ2669790.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            59456..59947
                     /gene="ptsN"
                     /locus_tag="OHO85_000360"
     CDS             59456..59947
                     /gene="ptsN"
                     /locus_tag="OHO85_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCQ2669791.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            59993..60847
                     /gene="rapZ"
                     /locus_tag="OHO85_000361"
     CDS             59993..60847
                     /gene="rapZ"
                     /locus_tag="OHO85_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCQ2669792.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60844..61116
                     /gene="npr"
                     /locus_tag="OHO85_000362"
     CDS             60844..61116
                     /gene="npr"
                     /locus_tag="OHO85_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCQ2669793.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            61330..61962
                     /gene="yrbL"
                     /locus_tag="OHO85_000363"
     CDS             61330..61962
                     /gene="yrbL"
                     /locus_tag="OHO85_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCQ2669794.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(61959..62687)
                     /gene="mtgA"
                     /locus_tag="OHO85_000364"
     CDS             complement(61959..62687)
                     /gene="mtgA"
                     /locus_tag="OHO85_000364"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCQ2669795.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(62684..63337)
                     /gene="elbB"
                     /locus_tag="OHO85_000365"
     CDS             complement(62684..63337)
                     /gene="elbB"
                     /locus_tag="OHO85_000365"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCQ2669796.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(63567..65903)
                     /gene="arcB"
                     /locus_tag="OHO85_000366"
     CDS             complement(63567..65903)
                     /gene="arcB"
                     /locus_tag="OHO85_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCQ2669797.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65999..66928)
                     /locus_tag="OHO85_000367"
     CDS             complement(65999..66928)
                     /locus_tag="OHO85_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCQ2669798.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            67603..72063
                     /gene="gltB"
                     /locus_tag="OHO85_000368"
     CDS             67603..72063
                     /gene="gltB"
                     /locus_tag="OHO85_000368"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCQ2669799.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPHIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            72076..73494
                     /gene="gltD"
                     /locus_tag="OHO85_000369"
     CDS             72076..73494
                     /gene="gltD"
                     /locus_tag="OHO85_000369"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCQ2669800.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            73678..74805
                     /locus_tag="OHO85_000370"
     CDS             73678..74805
                     /locus_tag="OHO85_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCQ2669801.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(74865..75329)
                     /locus_tag="OHO85_000371"
     CDS             complement(74865..75329)
                     /locus_tag="OHO85_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCQ2669802.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMAGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(75326..76201)
                     /gene="nanK"
                     /locus_tag="OHO85_000372"
     CDS             complement(75326..76201)
                     /gene="nanK"
                     /locus_tag="OHO85_000372"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCQ2669803.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(76198..76887)
                     /locus_tag="OHO85_000373"
     CDS             complement(76198..76887)
                     /locus_tag="OHO85_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCQ2669804.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(76935..78425)
                     /gene="nanT"
                     /locus_tag="OHO85_000374"
     CDS             complement(76935..78425)
                     /gene="nanT"
                     /locus_tag="OHO85_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCQ2669805.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(78534..79427)
                     /gene="nanA"
                     /locus_tag="OHO85_000375"
     CDS             complement(78534..79427)
                     /gene="nanA"
                     /locus_tag="OHO85_000375"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCQ2669806.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(79549..80340)
                     /gene="nanR"
                     /locus_tag="OHO85_000376"
     CDS             complement(79549..80340)
                     /gene="nanR"
                     /locus_tag="OHO85_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCQ2669807.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            80720..82087
                     /gene="dcuC"
                     /locus_tag="OHO85_000377"
     CDS             80720..82087
                     /gene="dcuC"
                     /locus_tag="OHO85_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCQ2669808.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(82130..82627)
                     /gene="sspB"
                     /locus_tag="OHO85_000378"
     CDS             complement(82130..82627)
                     /gene="sspB"
                     /locus_tag="OHO85_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCQ2669809.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(82633..83271)
                     /gene="sspA"
                     /locus_tag="OHO85_000379"
     CDS             complement(82633..83271)
                     /gene="sspA"
                     /locus_tag="OHO85_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCQ2669810.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(83666..84058)
                     /gene="rpsI"
                     /locus_tag="OHO85_000380"
     CDS             complement(83666..84058)
                     /gene="rpsI"
                     /locus_tag="OHO85_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCQ2669811.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(84074..84502)
                     /gene="rplM"
                     /locus_tag="OHO85_000381"
     CDS             complement(84074..84502)
                     /gene="rplM"
                     /locus_tag="OHO85_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCQ2669812.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(84721..85848)
                     /gene="zapE"
                     /locus_tag="OHO85_000382"
     CDS             complement(84721..85848)
                     /gene="zapE"
                     /locus_tag="OHO85_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCQ2669813.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMMRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            86042..86440
                     /locus_tag="OHO85_000383"
     CDS             86042..86440
                     /locus_tag="OHO85_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCQ2669814.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            86594..87961
                     /gene="degQ"
                     /locus_tag="OHO85_000384"
     CDS             86594..87961
                     /gene="degQ"
                     /locus_tag="OHO85_000384"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCQ2669815.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            88051..89118
                     /gene="degS"
                     /locus_tag="OHO85_000385"
     CDS             88051..89118
                     /gene="degS"
                     /locus_tag="OHO85_000385"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCQ2669816.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(89180..90118)
                     /gene="mdh"
                     /locus_tag="OHO85_000386"
     CDS             complement(89180..90118)
                     /gene="mdh"
                     /locus_tag="OHO85_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCQ2669817.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPSVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            90553..91023
                     /gene="argR"
                     /locus_tag="OHO85_000387"
     CDS             90553..91023
                     /gene="argR"
                     /locus_tag="OHO85_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCQ2669818.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT        22467 a        24444 c        22620 g        21636 t
ORIGIN      
        1 tatttctacc tgtataatct ttaaagatgt tgaacatata ttcacattaa atatgattat
       61 gtacttgtta caaggataag gttataaatg aataaagtta caaaaacagc tattgcgggc
      121 ttacttgcgc ttttcgcagg taatgccgct gcaaccgatg gtgaaatagt ttttgatggc
      181 gagattttaa aatccgcttg tgaaatcaat gactctgata agaaaattga agttgctctt
      241 ggtcactata atgccgaaca gtttcgttct gtaggcgatc gtagcccaaa aattccattc
      301 actattcctt tagttaactg cccagtgact gggtgggagc acgataacgg caacgttgaa
      361 gcgtctttcc gtttatggct ggaaacgcgg gataatggta ccgtccctaa cttccctaac
      421 ttggcaaaag tgggctcttt tgctggcaca gcagcaacag gtgtgggtat ccgtattgac
      481 gatgcggaaa gcggaaacct tatgccacta aatgctatgg gcaatgataa cacagtttat
      541 cagatccctg cggactccgc tggaattgtc aatgttgacc tcatcgctta ctacgtatca
      601 accgttgaag catcagaaat caccccaggg gaagcagacg ctgtcgttaa cgtaacgctg
      661 gattaccgtt aatacgttac ggcgttatct gacctttcag ataacgccct tttccttcct
      721 ctttctcgtt gtatcaggtt gaaaaataac tatgtcaaaa cgaacattcg cggtgataat
      781 aaccttgttg tgtagcttct gtatcggcca ggcgcttgca gggggtatcg ttttacagcg
      841 aacgcgagtg atctatgatg ccagccgcaa agaggctgcg ttacctgtcg caaacaaagg
      901 cgcagaaacg ccttatttac tgcaatcatg ggtagataat atagatggta caagccgtgc
      961 cccatttatt ataaccccac cgctatttcg tcttgaagct ggcgatgatt catcactgcg
     1021 aattattaaa acagctgata acctgcctga aaataaagag tcgctgttct acattaatgt
     1081 tcgtgccatt ccagcaaaga aaaaatcaga taatgttaat gctaacgagt tgacgctggt
     1141 atttaaaaca cggatcaaaa tgttttatcg ccccgcacac ctgaagggac gggtaaacga
     1201 tgcgtggaaa tcactggaat ttaaacgtag tgaccattca ctcaatatat ataacccaac
     1261 tgaatattac gtcgtatttg ccggactggc agtcgataaa accgatctca caagcaaaat
     1321 tgaatatatc gcacccggag aacataaaca gttaccactt cctgcatctg gcggaaagaa
     1381 cgtgaaatgg gctgcgatca atgattatgg cggcagttcc gggacagaaa ctcgtccact
     1441 gcaataacaa atataaaaaa cacaggtcat cagggaatgc cacaacgaca ccaccaggga
     1501 cataaacgca caccgaaaca gttggcgctc attatcaaac gctgtttgcc gatggtgctc
     1561 actggcagcg gcatgctttg cactaccgct aacgccgaag agtattattt cgaccccatt
     1621 atgctggaaa ccacaaaaag tggtatgcaa acaaccgatc tgtcacgatt ttcaaagaaa
     1681 tacgcacaac taccaggaac ttatcaggtt gatatctggc tgaataaaaa gaaggtttca
     1741 cagaaaaaaa ttacatttac cgccaatgcc gagcaacttc tgcagccaca gtttacggtt
     1801 gaacaactac gtgagctggg tattaaggtg gatgaaatcc cggcgctggc tgaaaaagat
     1861 gacgatagcg tgatcaactc gcttgaacaa atcattcccg gtacagctgc tgaatttgat
     1921 ttcaatcatc agcgacttaa tttgagcatt ccccaaattg cactgtaccg tgatgcaaga
     1981 ggttacgtct ccccttctcg ttgggacgat ggtataccaa cgctgtttac caactactcg
     2041 tttacaggtt ctgataaccg ttaccgccag ggcaatcgta gccaacgaca gtacctgaat
     2101 atgcaaaatg gtgctaattt tggcccctgg cgattacgca actattccac atggacacgc
     2161 aacgatcaga cgtcaagctg gaacactatc agtagttatt tacaacgtga tatcaaggcg
     2221 ttgaaatctc agttgcttct gggagaaagc gccaccagcg gcagtatttt ttccagctac
     2281 acctttactg gcgtgcaact cgcttccgac gataatatgt tgccaaacag ccagcgcgga
     2341 tttgccccaa cggtacgcgg tatcgcaaac agtagtgcaa tcgtgactat caggcaaaat
     2401 ggttatgtga tctatcaaag caacgtgcca gcgggtgcct ttgaaattaa cgatctctac
     2461 ccctcttcca acagcggcga tttagaagtc acgattgaag aaagtgacgg tacgcaacgt
     2521 cgctttatcc agccttattc ttcattaccc atgatgcagc gacctgggca tctaaaatat
     2581 agcgcgaccg ctggacgcta tcgcgctgat gcaaacagtg atagcaagga acccgaattt
     2641 gctgaagcca cggcaatata tggtttgaat aatactttta cgctgtatag tggcctgctc
     2701 ggttctgaag attattatgc gctggggatc ggtatcggcg gcacacttgg cgcactgggc
     2761 gcgttgtcga tggatatcaa cagagctgac acccaattcg ataaccagca ctcttttcat
     2821 ggctatcaat ggcgtacgca gtacatcaaa gatatcccgg aaaccaacac caatatcgct
     2881 gtcagctact atcgctatac caacgatggc tattttagtt ttgatgaagc caatacccgc
     2941 aattgggact ataacagtcg ccaaaaaagt gaaattcaat tcaacatcag ccagacaata
     3001 tttgatgggg taagtctgta tgcctccggt tcacagcaag actattgggg caataacgag
     3061 aaaaacagga atatctctgt tggggtttcc ggccagcaat ggggaattgg ttacagcctg
     3121 aattatcaat acagccgcta cactgatcaa aataatgacc gtgcactctc tttgaatctc
     3181 agtattccgt tagaacgctg gttaccgcgt agccgggttt cctatcagat gaccagccag
     3241 aaagatcgcc caacccaaca tgaaatgcgt cttgatggtt cactgctgga tgatggtcgc
     3301 ctgagctata gcctggaaca aagtctggat gacgataaca accataacag tagcgtgaac
     3361 gccagttacc gttcacctta tggaaccttc agtgccggat acagttacgg taatgacagt
     3421 agccaataca attacggcgt taccggcggc gtggttatcc atcctcatgg tgtgacgctc
     3481 tcgcaatatc tgggcaacgc ttttgcgctt attgatgcta acggggcatc tggcgtgagg
     3541 atacaaaact atccggggat tgctactgat ccctttggct atgcagtggt tccttatctc
     3601 acgacttatc aggaaaaccg tctctcggta gatactacgc agctgcccga taacgtcgat
     3661 cttgagcaaa caacacagtt tgtggtgccc aacagaggtg caatggtagc ggcgcgtttc
     3721 aacgccaata tcggttatcg cgtacttgtt acagtcagcg atcgcaacgg taaaccgttg
     3781 ccctttggcg ctcttgccag caacgatgag acggggcaac aaagtatcgt cgatgagggc
     3841 ggcatactat atctctctgg gatatcgagt aaatcacaaa gctggactgt acgctgggga
     3901 aatcaggcag atcaacaatg tcagtttgct tttagtacac cggattcaga accaacaacc
     3961 tctgtattac aaggcacagc gcagtgccat taaggataaa aaaatgaaaa gagcgcctct
     4021 tataacagga cttttgttga tatccacatc ctgcgcttat gcctcctcag gagggtgtgg
     4081 agccgacagc actagcggtg cgacaaatta cagcagtgtg gttgatgatg ttacggtgaa
     4141 ccagacagat aacgtgacag gacgggagtt tacctctgca acgctaagta gcactaactg
     4201 gcaatacgcc tgttcctgct ctgcgggtaa ggcagttaaa cttgtctata tggtcagccc
     4261 tgtactcacc accactggac atcaggcagg atattacaaa ctcaatgata gcctggatat
     4321 taaaaccacc ttaaaggcga atgacattcc aggactcgtg actgaccaaa ccgtttctgt
     4381 taacacccga ttcacacaga taaaaagcaa caccgtatat tctgctgcaa cccaaacggg
     4441 tgtttgccag ggtgacacgt ctcgttatgg acccgttaat attggtgcaa ataccacctt
     4501 taccctgtat gtcaccaagc catttctcgg ctcgatgacc attccgaaaa cggatattgc
     4561 cgtcattaaa ggcgcgtggg tcgatggaat gggaagcccg tctacaggtg acttccatga
     4621 tttagtcaag ttatcgattc aaggaaatct caccgccccg cagtcgtgca aaattaatca
     4681 gggcgatgtt attaaggtta attttggatt catcaatggt cagaagttta ccacccgcaa
     4741 tgccatgcca gacggtttta ctccagtaga ctttgatatc acttatgact gtggtgatac
     4801 ttcaaagatt aaaaactcgt tgcaaatgcg catcgacggt acaactgggg tagtagacca
     4861 gtacaacctt gtcgccagac gaagaagttc agacaatgcg cccgatgtcg gtattcgtat
     4921 tgaaaatctc ggcggcggag ttgcaaatat tccttttcag aacggtatcc ttcccgttga
     4981 tccttccggg catggcaccg tcaatatgcg cgcctggcca gttaatctgg tcggtggtga
     5041 gctggaaaca ggaaaatttc agggcacagc caccattacc gtcatcgtgc ggtaaaagaa
     5101 gacgttgcgt tttgattaac gcaacgcttc ggcactctta cccctgctgc tccagcgcat
     5161 acttatacag cgcatttttc ttcacgccat gaatctctgc ggccagcgcc gccgcttttt
     5221 tcagcggtag ttctgcctgt agcagcgcca gcgtgcgcag ggcatcggcg ggtaagtctt
     5281 cttcctgcgc tttatggcct tcgacaatca gcaccatttc gcctttgcga cggttttcat
     5341 cttctttgac ccacgccagc agctcgccaa cgggcgcgcc gtgaatggtt tcccaggttt
     5401 tggtgagctc acgcgccaga accacgtagc gggattcgcc taataccgca acgatatctt
     5461 ccaggctgtc taacagacgg tgggttgatt cataaaaaat cagagtgcgc ggctccgctt
     5521 caatggcttt tagcgcatca cggcggcctt ttgatttggc aggtaaaaag ccttcgtaac
     5581 agaaacggtc agagggtaaa cccgcggcgc ttaacgcagt gatagcagca cacggcccgg
     5641 gtagcggcac cacgcgaatc cccgcttcac ggcaggtacg caccagatgg tagccaggat
     5701 cgttaattag cggcgttccg gcatcggaaa ccagcgcaat gttttgcccc tcttgcagct
     5761 tcgccagcag cgtttcggct ttttgttgtt cgttatggtc gtgcagcgca aacaaccggg
     5821 cattaatccc aaaatgttgc agcaataaac cggtgtgacg agtatcctcg gcggcaatca
     5881 gatcaacggc ctgtaatacc tctaacgcac gctgggtgat atccgccaga ttgccgattg
     5941 gcgtcggtac aatgtaaagc tggccctgag aattatccgc cgattggtgt tgtttcattg
     6001 tgtcgtccgt attgccgatt taatattgag cattgcgtaa aaaaaatatc actggataca
     6061 ttatggtacc ctcaacattt tctcgtttga aagccgcgcg ttgtctgcct gttgttctgg
     6121 cagccctgat tttcgccggt tgtggcaccc atactcccga tcagtccact gcttatatgc
     6181 agggcacggc gcaggctgat tctgcctttt atcttcagca gatgcagcaa agctctgatg
     6241 ataccaggat caactggcaa ttactcgcca ttcgtgcact ggtgaaagaa ggtaaaaccg
     6301 ggcaggcggt ggagttgttt aaccaactac cgcaagaact gaacgattct cagcgtcgcg
     6361 agaaaacact gctggcggta gagattaaac tggcgcagaa agattttgct ggcgcgcaaa
     6421 atttgctggc gaaaatcaca cctgccgatt tagaacaaaa ccagcaagcc cgttactggc
     6481 aggcaaaaat cgatgccagc caggggcgtc cttccattga tttactgcgc gcgttaattg
     6541 ctcaggaacc gctgctcggc gcgaaagaaa aacagcagaa tattgatgcc acctggcagg
     6601 cgctctcctc catgactcag gaacaggcga atacgctggt gatcaacgcc gacgaaaata
     6661 ttctgcaagg ctggctggat ctgcagcgcg tctggtttga taaccgaaac gatcccgaca
     6721 tgatgaaagc cgggatcgcc gactggcaga aacgttatcc gaacaatccg ggcgcgaaaa
     6781 tgctgccaac gcagttggtt aacgtaaaag cgtttaaacc agcctcgacc aacaaaatcg
     6841 ccctgctgtt gccgctgaat ggccaggcag cggtatttgg tcgcactatt cagcaaggct
     6901 ttgaagcggc gaaaaatatc ggcactcagc cagtggcggc tcaggtagct gccgcacctg
     6961 ccgcagacgt agcagaacaa cctcagccgc aaaccgtgga tggcgttgcc agcccggcac
     7021 aagcctcggt tagcgatctg accggtgaac agcctgcagc ccagccggtg cctgtaagcg
     7081 ccccggcgac aagcaccgca gcggtaagcg cacccgcaaa tccatccgca gagctgaaaa
     7141 tctacgatac ctcatcacaa ccacttagcc agatcttaag ccaggttcag caggatggcg
     7201 cgagtattgt ggtcggtccg ttgctgaaaa ataacgttga agagttgctg aagagcaaca
     7261 ccccgctgaa cgtgctggca ctgaaccagc cggagaatat cgaaaaccgc gtcaatattt
     7321 gttacttcgc gctttcacct gaagacgaag cgcgcgatgc agcgcgtcat attcgtgacc
     7381 agggtaaaca agcgccgctg gtgctgatcc cacgcagttc attgggcgat cgcgtagcca
     7441 atgcgtttgc gcaagagtgg cagaaactgg gcggcggcac cgttctgcaa caaaaatttg
     7501 gttccaccag cgaattacgc gcgggtgtta acggcggttc tggtattgct ttaacgggta
     7561 gcccgattac tcccagagcg acaaccgact ccggcatgac gaccaacaat ccaacgctgc
     7621 aaaccacgcc aaccgatgac cagttcacca ataatggcgg tcgtgtcgat gcggtgtaca
     7681 ttgtggcaac gccgggtgaa atcgctttta tcaaaccgat gatcgccatg cgtaacggta
     7741 gccagagcgg tgcaacgctg tacgccagct cccgcagtgc gcaagggacc gctggcccgg
     7801 atttccgact ggagatggaa ggcttgcagt acagcgaaat cccgatgctg gcaggcggta
     7861 atctgccgtt aatgcagcag gcgctcagcg cggtgaacaa cgattattcg ctggctcgca
     7921 tgtatgcgat gggcgttgat gcctggtcgt tggcaaatca tttctctcaa atgcgccagg
     7981 ttcagggttt tgaaatcaac ggtaataccg gaagcctgac ggcaaacccg gattgcgtga
     8041 ttaacaggaa gttatcatgg ctacagtacc aacaaggtca ggtagtcccc gccagttaac
     8101 caccaaacag accggcgatg cgtgggaagc acaagcgcgt cgctggctgg aaggcaaagg
     8161 actgcggttt atcgccgcta acgtgaacga gcgtggcggc gagatcgatc tgataatgcg
     8221 tgaaggccgg accaccgttt ttgtcgaggt acgctatcgc cgctctgcgc tttatggcgg
     8281 cgcggcagcc agtgtgaccc gcagcaaaca acacaaatta ttacagactg cccgcttgtg
     8341 gctcgcgcgt cataatggga gttttgatac tgtggattgc cggttcgatg tggtagcctt
     8401 caccgggaat gaggttgagt ggattaagga tgcctttaat gaccactcat aattaaggtt
     8461 taaggattag cgtgcaagaa agaattaaag cttgcttcac tgaaagcatt caaactcaaa
     8521 ttgcggcggc agaggcgctt ccggatgcca tctcccgtgc agccatgacg ctggttcagt
     8581 ctctgctcaa tggcaacaaa atcctctgtt gtggtaatgg aacttccgct gccaatgcac
     8641 agcattttgc tgccagcatg atcaaccgtt tcgaaacgga gcggcccagc ttacctgcca
     8701 ttgcactaaa tactgataat gttgtcttaa cggcgattgc caacgatcgc ttacatgatg
     8761 aagtgtatgc aaaacaggtg cgggcgctgg gtcatgcggg agatgtattg ttagccattt
     8821 ccacgcgtgg caacagccgc gatattgtaa aagcggtaga agctgccgtt acgcgtgata
     8881 tgaccattgt ggcattaacc ggctatgacg gcggtgaact tgcaggtttg ttagggccac
     8941 aggatgtgga aatccgcatt ccttcgcatc gtagtgctcg cattcaggaa atgcatatgc
     9001 tgacggtaaa ttgcctgtgc gatctgatcg ataacacgct tttccctcac caggatgatt
     9061 aaggagaata catgaaggca ttatcgccaa tcgcagtcct tatttccgcg ctgctgttgc
     9121 aaggttgtgt tgccgctgcc gtagtgggta ccgctgctgt gggtaccaaa gccgcaactg
     9181 acccacgcag tgtcggcacc caggtggacg atggtaccct ggaagtgcgc gtgaacagcg
     9241 cattgtcgaa agacgaacag attaagaaag aagcgcgcat taatgtaacg gcctatcagg
     9301 gcaaagtgct gctggttggg cagtcaccaa acgctgaact ttcggctcgc gccaaacaga
     9361 ttgctatggg cgtagacggt gccaacgaag tgtataacga gattcgtcag ggccaaccga
     9421 ttggtctggg cgaagcgtct aacgatacgt ggatcaccac caaagtgcgt tcgcagctct
     9481 taaccagcga cctggtgaaa tcgtccaacg tgaaagtgac caccgaaaac ggtgaagtgt
     9541 tcctgatggg gctggtgact gaacgtgaag cgaaagcggc ggcagatatt gccagccggg
     9601 taagcggcgt gaaacgggta actacggcgt ttacgtttat taaatagcag ccccttgtaa
     9661 tgcctgatgc gacgcttgcc gcgtcttatc aggcctacaa cgacacaaaa cgtaggtcag
     9721 ataaggtgtt tacgccgcat ccgactgctg ttgcctgatg cgacgcttgc cgcgtcttat
     9781 caggcctaca acgatacaaa ccgtaggtca gataaggcgt ttacgccgca tccgacatgt
     9841 tttccctcaa atcagaacaa cagcgccagc ccgccgacaa tcacaccaga cactgccacc
     9901 atcgcccccg tcagccataa ggctttcgcc gggaacgctt tgcgcagcat aatcagtgac
     9961 ggcaaactca ccgccgggag cgtcatcaac aatgccagcg ccggagcagt tcccatacct
    10021 gccagcatca tcgtttgtac aatcggaatt tctgctgccg tgggaatgac aaacaagcat
    10081 cctgccaccg ccatcgccac cacccacatc aggctgttat cgacagtacc atcggcatgg
    10141 gggaataacc agacgcgagc ggcacccaac accagtactg caaggatgta aaccgggatc
    10201 gtactccaga aaagcgtcca tagcgccctg ccccagcggc taaaaaaacc gccctgtgct
    10261 tccggtatgt caatttcgac cggtgcctgc gtttgcggtg tttcacgcac ccatttttgc
    10321 accagcgtcg caatcagcaa caccatcacc agcccggcca ccagacgaat cgccgcaaaa
    10381 ccccagccga ggacaaagcc cataaacacc agcgtcgccg ggtttaacac cggattgccc
    10441 atccagaatg ccagcgcacc gcccatcgac acctgttggc gacgcattcc cgccgcgacc
    10501 ggagccgcac agcaggtaca catcatgccc ggcaacgaaa acagcgttcc cagcagcgtg
    10561 ccgcgaaagc gcgattgccc aagcgtacgc aacaaccagt cacgcgggat cagcacctga
    10621 atcaacgaac cgaggatcac ccccagcacc gccgctttcc ataccgcgag gaaatagatc
    10681 atcgcgtaat ccaacgccgc ctgccatggg ttagcatccg cctgcgcaag gatagattta
    10741 ccgatactgt gggtttcggc agcagtaaac gctttgccgt agtaaggttc ccatttcaca
    10801 taccagagac cggcaatgac aacgagaaag aaaagcgcgg gtttccacca ctgaatgggc
    10861 gttgccgcct gagatgaaga ctgaccagtc atagcattcc ccaggaataa gttatgataa
    10921 ttgagcgcgt gaatattacg ctcactatca attcttggga ataattattc agctcttttg
    10981 cgtaattctg aagagcttaa aatcgtcacg ccttcatgct caggccgcat cgactctgcc
    11041 agcatgaccc gcgccacgtc tctcgcatca atggatttcc agttacctgg taacaaacgg
    11101 aacagcggcg caaaaagcgt ttcgttcatc cgctgtttgc tacgatcgcc cagtaacatc
    11161 gacgggcgag caatggtcaa tttcggccag ttctgggcaa ttaatgcttc ttccatctcc
    11221 cctttgacgc ggttatagaa aaacggcgag tgggcattgg cacccatagc actgaccacc
    11281 agcatatgct gcgcgcccag ccgccgcccg gttaatgcgg tatccactac cagcgtgtaa
    11341 tcggcatgaa taaacgcctc tttgctcccc gcttctcgcc gcgtggtgcc gagacaacaa
    11401 aacacaatgt cgatgggatc ggtgacctgc gccagcgcgt cagtcagttg cggatcatgg
    11461 ggattgaaca ctcctggcat atctcccaac ggacgtcgcg tcggtgcagc aatagcgtta
    11521 actttcggtt cgttaatcaa catccgcagc aggtgaccgc ccaccagccc cgttgcgcct
    11581 gtaatcagta cctgactcat cttcgctcct ttacagaatt gtctgccttg cgctccccgg
    11641 ctcatgcacc aggcttaata gaccatgagg taattatccc cgattgtgag aaattcgcct
    11701 catccaatgc aacaacgcgg aggaagcatg agtaagaaaa ttgccgtttt aatcactgat
    11761 gaatttgagg attcagaatt tacttcaccc gcagacgagt tccgtaaagc cggacacgaa
    11821 gtgattacca ttgaaaaaca agcgggtaaa acggtgaaag gcaaaaaagg agaagccagc
    11881 gtgaccatcg ataaatccat cgatgaagtg acgccagcgg agtttgatgc cctgttgcta
    11941 ccgggcggtc attcaccgga ttatctgcgt ggcgacaacc gttttgtcac ctttacccgt
    12001 gattttgtga atagcggcaa acctgttttc gccatctgtc acggtccgca gttgctgatc
    12061 agcgccgatg tgattcgggg gcgcaaactg accgcagtta aaccgatcat tattgatgtt
    12121 aaaaatgcgg gcgcggaatt ttacgatcag gaagtcgtgg ttgataaaga tcagctggtt
    12181 accagccgga caccggacga tctgccagcg tttaaccgcg aggcgttacg cctgctcggt
    12241 gcctgagtcg cgtagccaaa tcattttttt accaaagccc agcgtgttgt cggtgaattt
    12301 gatttcatcg agccggattt cccacaccgg ggccgacagc attctggcaa ccggaaagcg
    12361 acgattgtac gccttgcgcg cgaggtcgct ttcctcacct tccagcctgc ggatctcacc
    12421 tttaaactgc acaccgcgaa ttaacgctac cgttttcggc tgaccgttta ccgttccggc
    12481 aactgccgcc tgcggcccgc tcatctgcgc gtggcgcgtt ttttcttccg tcaaaatgta
    12541 gaaggcgact ttctgcgcat caaaaagata aaaggcatta gcgcaccaga gttccccttc
    12601 ctgctgcaca caccaggtga caacatgttg ttttgccagc caacggctga tggcgatgag
    12661 tgtttccatt gctgttctct tttatactgt gggcctgacc ttaacatgcg atgcagacga
    12721 tgacaccctg gtttctttac ttgatccgta ccgccgacaa taagctttat accgggatca
    12781 ccacggatgt cgaacgccgc tatcagcagc accaaagcgg caaaggggcg aaagcactgc
    12841 gcgggaaagg agaactgacg ctggcgtttt ctgctccagt gggcgatcgt tcgctggcgt
    12901 tacgggcgga atatcgcgtt aagcaactga ccaaacggca aaaagagcgt ctggtagcgg
    12961 aaggcgcagg gtttgcagag ctgttaagca gtctgcaaac cccggagatt aaaagcgatt
    13021 gaaatgctcg tgatactcaa ccaggccggt aacgccattc agcgcgtcat ccgctaaacg
    13081 atgtacctgg aaggcgcttt cagtgcccgg ccagcggcaa cgcagatcgt gatgcgccgc
    13141 cagttcaaag ccgaaacgac tgtacagcgc cggatcgccc agcgtcacca ctgcggcata
    13201 gccgaactca ttaagcgaat cgagtccttc atagaccagt tggcgtgcca gcccctgtcc
    13261 acggtatttt tcatcaaccg ccaacggtgc catgccgacc cattgcaggt cttcgccctg
    13321 cacatcaacc ggactaaatg ccacatagcc aatgacctga ccttcgtcat ctgtcgccac
    13381 cagccccagc gtcagaaagc catcttcacg cagatcgtga accagcttcg cttccgcatc
    13441 actttcgaat gagcgacgca gcagggcatc aatacccggc gcatcaatgg gaatttctac
    13501 tcgaattagc atggttcacc taccgatgtc tgtttggttt caggcgcggt tttcattccc
    13561 gcctcaacaa aatccgccag ttgcagcagc atcatgcgta aggctttcgg catttgctcc
    13621 agttcaatgg cgtccatcag gtttttcaca tacagcccca gctccgtatc gccttcaatc
    13681 accagccgac gctggaagaa gagcgtatcc ggatcttgtt tacgcgccgc aatcatcagc
    13741 agatcgctgg cgtcggcact aaaactcaca tcagcttgcg cgttctggct gaccaccagt
    13801 ttgccattca ccaccgaggt aaaccattgc aggtcaatat cacgcacatg aatacttaac
    13861 cagcggcctt caagaaactc cagctcgcca tcatccagcg cctgacggaa ttgccagctt
    13921 aagacctgct caagaacctg gcgttttagc gcaaatggcg tcagttttac cggtacactc
    13981 aacagagatg gccccaaatg cacaatacgg gaacgcagtt tatccaacac gagttttact
    14041 ccctgtttca acaatcatcc tattttgcca tatcagaaaa ataacatagc ggtataaatc
    14101 aacaattcca tatgaaattg ctgctaccac caatacaact ttaactgcct taaatcaaaa
    14161 attgtcgcag caaggttaac taaaatccca gttcgttaac atttttgcgt tttgatagcg
    14221 caaccttcag gaaaaattat ggagctgctc tgccctgccg gaaatctccc ggcgcttaag
    14281 gcggccatcg aaaacggcgc agatgctgtt tatatcgggc taaaagatga tactaatgcc
    14341 cgtcacttcg ccggccttaa ctttaccgag aaaaaattgc aggaagcggt gagttttgtc
    14401 catcaacatc gccgcaaact tcacatcgcg attaacactt ttgcgcatcc ggacggttac
    14461 gcccgttggc agcgcgccgt ggatatggcg gcgcagctgg gtgccgacgc gctgatcctc
    14521 gccgacctcg ccatgctgga gtacgccgcc gagcgttacc cgcatattga gcgtcatgtg
    14581 tcagtgcagg cttcggcgac caatgaagag gcgattaact tttatcatcg ccattttgac
    14641 gttgctcgcg tggtgctgcc gcgcgtgttg tcgattcatc aggtgaaaca gctggcacgg
    14701 gttactcctg tcccgctgga agtgtttgct ttcggcagcc tgtgcattat gtcggaaggt
    14761 cgttgctatc tgtcgtcgta tctgacgggt gagtcgccca acaccgtggg agcgtgttct
    14821 ccggcccgtt tcgtgcgctg gcaacaaacg ccgcaggggc tggaatcccg cctgaacgaa
    14881 gtgctgatcg accgttatca ggacggcgaa aacgcaggtt atccgacgct gtgtaaaggg
    14941 cgttatctgg tggacggcga gcgctatcac gcgctggaag aaccaaccag tctcaatacc
    15001 ctggaactgc tgccggagtt aatggctgcg aatattgctt cggtgaaaat tgaaggccgc
    15061 cagcgtagcc cggcgtatgt cagccaggtg gcgaaagtct ggcgtcaggc tatcgaccgt
    15121 tgtaaggccg atccgcaaaa ctttgtaccg caaagcgcgt ggatggagac gctcgggtcg
    15181 atgtccgaag gcacgcaaac cactcttggc gcatatcacc gtaaatggca gtgagaaaag
    15241 caatgaaata ttccttaggg ccagtgctgt ggtactggcc aaaagagacg ctggaagaat
    15301 tttatcagca ggccgccacc agcagcgccg acgtgattta tcttggtgaa gcggtatgca
    15361 gcaagcgtcg ggcaaccaaa gttggcgact ggctggagat ggcaaaatcg ctcgccggga
    15421 gtggtaagca gattgtgctc tccacgctgg cgctggtgca ggcatcatct gaactgggcg
    15481 aactgaaacg ctatgttgag aacggtgagt ttctgattga agccagcgat ctcggcgtgg
    15541 tgaatatgtg cgctgaacgc aaactgccgt tcgtcgccgg gcacgcgctg aactgctaca
    15601 acgcggtgac actgaaaata ttgctcaaac agggcatgat gcgctggtgt atgccagtgg
    15661 agctttcccg cgactggctg gtgaatctgc ttaatcagtg cgatgagctg ggcattcgta
    15721 accagtttga agtggaagtc ctgagctacg gtcatctgcc gctggcctac tccgcccgct
    15781 gctttaccgc gcgttcggaa gaccgcccga aagatgagtg tgaaacctgc tgcattaagt
    15841 atccgaacgg gcgcaacgtg ctgtcgcagg aaaaccaaca agtgtttgta ctcaatggca
    15901 ttcagaccat gagcggctac gtttacaacc tcggtaacga gctggcatcc atgcagggcc
    15961 tggttgatgt ggtccgcctg tcaccgcagg gtactgacac tttcgcgatg ctcgacgcct
    16021 tccgcgctaa tgaaaatggc gctgcaccac tgccgttgac ggcgaacagc gactgtaacg
    16081 gctactggcg gcggctggcc gggctggagc tgcaagccta agtaaatatg ctcgtcatac
    16141 ttcaagttgc atgtgctgca tctgcattcg ttcaccccag tcacttactt atgtaagctc
    16201 ctggggattc actctcttgt cgccttcctg caactcgaat tatttagagc acagctcact
    16261 ttgttaacaa ctttaactac tctttaatgc agtattaaag attaatcggt aacaaagtga
    16321 gctgttatga ctgataaaac cattgcgttt tcgctactcg atctggcccc cattcccgaa
    16381 ggttcttcag cgcgagaagc attctcccac tctctcgatc tcgcccgtct ggctgaaaag
    16441 cgcggctatc atcgctactg gctggcggaa caccacaata tgaccggcat tgccagtgct
    16501 gcaacatcgg tattgattgg ctatctggcg gcgaatacca ccacgctgca tctggggtct
    16561 ggcggcgtga tgttgcctaa ccactcaccg ttggtcattg ccgaacagtt cggcacgctc
    16621 aatacgctct atccggggcg aatcgatttg gggctgggtc gtgctccggg tagcgatcag
    16681 cgaaccatga tggcgctacg tcgtcatatg agcggcgata ttgataattt cccccgcgat
    16741 gtcgcggagc tggtggactg gtttgacgcc cgcgatccca atccgaatgt gcgcccggta
    16801 ccaggctatg gcgaaaaaat ccccgtgtgg ttgttaggct ccagccttta cagcgcgcaa
    16861 ctggcggcgc agcttggtct gccgtttgcg tttgcctcac acttcgcgcc ggatatgttg
    16921 ttccaggcgc tgcatcttta tcgcagcaac ttcaaaccgt cggctcgact ggaaaaacca
    16981 tacgcgatgg tgtgcatcaa tattatcgcc gccgatagca accgcgacgc tgagtttctg
    17041 tttacctcaa tgcagcaagc ctttgtgaag ttgcgccgtg gcgaaaccgg gcaactgccg
    17101 ccaccgattc aaaatatgga tcagttctgg tcgccgtccg aacagtatgg tgtgcagcag
    17161 gcgctgagta tgtcgttggt aggtgataaa gcgaaagtgc gtcatggctt gcagtcgatc
    17221 ctgcgcgaaa ccgacgccga tgagattatg gtcaacgggc agattttcga ccaccaggcg
    17281 cggctgcatt cgtttgagct ggcgatggat gttaaggaag agttgttggg atagtgtgtc
    17341 ttaacgcggg aagccttatc cgagctggca acgctgtcct acgtagacct gataagcgaa
    17401 gcgcatcagg cattgtgtag gcagcagaaa tgtcggataa ggcaccgctg attactgata
    17461 caccggcagt aaattaaagc tcgataaaat atggaccagt gcgttgccaa cgccaaacac
    17521 cagaatcagc gtaatcatcg gcttgccgcc ccagacgcgg aatttcgggc tgccaaagcg
    17581 tttacgcgat gcgcgggcta acagcgccgg aacaattgcc gcccagatgg tagctgctaa
    17641 accagcataa ccaatggcgt acaggaatcc gttcgggaac agtagaccac ccacaacagg
    17701 cggggcaaag gtcagcaatg ccgttttcaa gcgccccagg gctgagtcat cgaaaccaaa
    17761 cagatctgcc agatagtcaa acaaacccag cgttacgcca aggaacgaac tcgctaccgc
    17821 aaagtttgag aacacgacca gcagaaggtc caggctacgg ctgttcagca cgccgcttaa
    17881 tgcctgtacc agcacatcaa tattaccgcc cttctctgca ataccgataa actccggacg
    17941 cgggatgtta cccatcgtcg ccagcaacca gatggtatac agcgccagtg ccatcagcgt
    18001 accgtacacc aggcatttca cgatggtttt cggatctttg ccgtaatact tcatcaggct
    18061 tggcacgtta ccgtgataac caaacgacgc caggcagaac ggcaatgtca tcagcagata
    18121 cggcgcataa gatgcattgc tttctgcaac gttgaacaat gtcgcaggct gcacatgccc
    18181 caacaggctg ccaaaggtga ggaagaaggt aatgactttc gcccccagca caatcgccgt
    18241 catgcggctg acggccttag tactcaacca caccacaaac gctaccagca gtgcaaaacc
    18301 aaaacccgcc gcccgtgccg ggacgtttag cgacatctct gcgaaggtgt gatgcagaat
    18361 cgaaccactg gcagaaatat aggcataggt caggatatag agcacaaagg caatggaaat
    18421 accgttgacc acgttccagc ctttgcccag caaatctttg gtgatggtgt caaaactcga
    18481 accgattcgg taattcaggt tagcttccag aatcatcaag ccggaatgca gcatacagaa
    18541 ccaggtaaag atcagcgccg ccattgacca gaaaaaccac gccccggaca tgaccactgg
    18601 cagagaaaac atccctgcgc caataatggt gccgccgata atcaccacgc cgccaagcag
    18661 cgacggtgac gtttgggtgg tggttagtgt tgccatgagg gcttctctcc agtgaaaaat
    18721 agtgcgactg cgttgttatg cattgcactg taccagtaca cgagtacaaa agacagaaaa
    18781 aaagccccga tggtaaaaat cggggctgta tatattattt tacagattgt gttcgctgtt
    18841 cagcgatgat tacgcatcac caccgaaacg acgacgaccg gtagagtcat cacgacgcgg
    18901 agcgcggcct tcacgacgtt cgccgctaaa acgacgacca tcaccacggc caccttcacg
    18961 gcgttcaccg ctgaagttac gaccgccttc acgacgttcg ccaccgaaac cacgaccacc
    19021 gccacgacgc tcaccgccag tatgcggctg tgcatcgccc agtaactgca tgttcatcgg
    19081 cttgttgaga atgcgagtgc gcgtaaagtg ttgcagcact tcacccggca tacctttcgg
    19141 cagttcgatg gtggagtgag aagcaaacag cttgatgtta ccaatgtaac ggctgctgat
    19201 gtcgccttcg ttagcaatcg caccaacgat atgacgaact tcaacaccat catcgcggcc
    19261 cacttcaatg cggtacagct gcatatcgcc aacatcacga cgttcacgac gcggacgatc
    19321 ttcacggtca ccacgcgggc cacggtcgtt acgatcgcgc ggaccacggt catcacggtc
    19381 acggaattca cgtttcggac gcatcggcgc atctggcggt acgatcagag tacgttcacc
    19441 ctgtgccatt ttcagcagtg ccgcagccag agtttcgaga tccagctctt caccttctgc
    19501 agtcggctga attttgctca gcagtgcgcg gtattgatcc agatcgctgc tttccagctg
    19561 ctgctgtact ttagcggcga atttttccag acggcgtttg cccagcagtt ctgcgttcgg
    19621 cagttctact tccggaatag tcagcttcat agtacgttca atgttgcgca gcagacgacg
    19681 ctcgcggttc tcaacgaaca gcagcgcgcg gccagcacga cccgcacgac cggtacgacc
    19741 gatacggtga acgtaagact cagaatccat cgggatatcg tagttaacta ccaggctgat
    19801 acgctcaacg tccaggccac gggctgcaac gtcggtcgca atcaggatgt ccagacgacc
    19861 atctttcagg cgttccagtg tctgttcacg aagcgcctgg ttcatgtcac cgttcagcgc
    19921 ggcgctgttg tagccgttac gctcaagagc ttcagccact tccagagtcg cgtttttggt
    19981 acgaacgaag ataatcgccg catcaaaatc ttccgcttcc aggaaacgta ccagtgcttc
    20041 gtttttgcgc ataccccaga cagtccagta gctctggctg atgtcaggac gggtagtcac
    20101 gctggactga atgcgcactt cctgcggctc tttcataaag cggcgggtaa tgcgacgaat
    20161 cgcttccggc atggttgcag agaacagagc ggtctgatga ccttccggga tctgcgccat
    20221 aatggtttca acgtcttcga tgaagcccat gcgcagcatt tcgtcagctt catccagaac
    20281 cagaccgctc agtttagaga ggtccagagt gccacgtttc aggtggtcca gcagacggcc
    20341 cggagtaccg acaacgatct gcggcccctg acgcagggcg cgtaattgca cgtcataacg
    20401 ctggccgccg tacagagcaa ccacatttac gccgcgcatg tgtttagaga aatccgtcat
    20461 tgcttcagca acctgtaccg ccagttcgcg ggtcggtgcc agcaccagaa tctgtggtgc
    20521 tttcagctca ggatcaagat tctgcaacag aggtaaagag aatgctgcag tttttccgct
    20581 ccccgtctgg gccataccca gaacgtcgcg gccattcagc agatgtggaa tacactctgc
    20641 ctgaattgga gatggttttt cgtaacccag atcgttaagg gcttcaagga taggagcctt
    20701 caggcccaga tctgcaaaag tggtttcgaa ttcagccatg tagtacgtgt gcctcaaaat
    20761 taatggcggc cagtctacat aactcatcat gaaattgatc agcaattttc attgaaaagt
    20821 gtgaaccggc tcaaagtagg tgtattaacg aacaacaacg ccctcacccg ttaaggtgat
    20881 ggcaatcaaa aaagattacg ggctgatgtg tacgtcagct attgctggtc cgattctgcc
    20941 aggtcatctt ggtcctggcc caggagcgat aattccaaca atgcgtatcg gtgctcaaca
    21001 aagttatgaa cgttgttggc aaccgccagt ttgaacagtg ccgtggcgct gtccaaatcc
    21061 cccagactta ggtagtactt acctaaatag aagttggttt cactgagatg ctcagcgagc
    21121 gaggtgttat ccgttgcgtc cgccttgagc ctttccatta acgtttgttc gctaatgttg
    21181 cccaggtaga actcgacaat gttccatccc cactgttcct tatccgattt ttcgaagtgc
    21241 tgtttcaaca cttctttagc ctgcttctca tcgagcttct gctcggcgag ataaagccac
    21301 agactacgga aaggatcatt gggatcgtct tgataaaacg ccagcagatc atcttgcgct
    21361 aacttgtcac gaccgccgta atataatgcg atcccgcgat tcaagtgcgc gtagttgtaa
    21421 gttggatcaa gctcaagtac agaatcaaac gcttcatagg cagcatcaaa attgcctgcc
    21481 tgcgttaaat atatgcctaa gtaattgaat acttcaggca tatccggtcg gattgccagc
    21541 gcttgcgaaa aatcgttacg cgctaatgcc ctcagaccga gactatcata caacactccg
    21601 cgctcatata aaagctgtgc gcgttcgtca tcggttaaag cccgactggc aaggatttgt
    21661 tccatacgtg ccagaatcac ttcctgctgt aaagtcggtt gcaatggtac cgcgaggact
    21721 tcacttttac gccaggaagt attactgcat cctgcaagcg taagtgctgt cgcaacgaaa
    21781 caccagcgca aaaaaggctt catttcccac tcccgaagac cacggttgaa tgaacgtcct
    21841 gttcccggtt gctaacaagg cgtcctgccc ggttaaaagc cccccgccgc agcggagggc
    21901 aaatggcaac cttactcgcc ctgttcagca gccggagctt ccggtgctgc agcaggttga
    21961 gactgctcag tcgcttcttt aatgctcaga cggatacggc cctggcgatc aacttccaga
    22021 actttcaccg gtacttcctg acccatctgc aggtaatcgg tcactttctc aacgcgtttg
    22081 tcagcgattt gagagatgtg gaccagacct tctttaccgc cgccgatggc aacaaatgcg
    22141 ccaaagtcaa cgatacgggt cactttacca gtgtagacgc ggcccacttc gatttctgca
    22201 gtgatctctt cgatacgacg aatagcatgt ttcgctttct cgccgtcggt cgctgcgatc
    22261 ttcacagtac cgtcatcttc gatttcgatg gtagtgccag tttcttcggt cagggcacgg
    22321 ataacagagc cgcctttacc gataacgtct ttgatcttat ccgggttgat cttgatggta
    22381 tggatacgcg gtgcgaactc agagatatcg ccacgcggcg cgttgatcgc ctgttccatt
    22441 acgcccagga tatgcagacg cgcaccttta gcctggttca gcgcaacctg catgatctct
    22501 ttggtgatac cttcaatttt gatatccatc tgcagtgcag agataccgtc gcgggaacct
    22561 gcaactttga agtccatatc gcccaggtga tcttcgtcgc ccaaaatgtc agacagtaca
    22621 acgtagttgt cgccttcttt caccagaccc attgcgatac ccgcaacggc agctttgatt
    22681 ggcacacctg cgtccatcag cgccagagac gcgccgcaca cggaagccat agaagaagaa
    22741 ccgttggatt cggtgatttc agacactaca cgtacggtgt acgggaattt gtccatatcc
    22801 ggcatgactg ccagcacgcc gcgcttcgcc agacgaccgt gaccaatttc acgacgcttc
    22861 ggagaaccga ccatgccggt ttcgcctacg gagtacggag ggaagttgta gtggaacagg
    22921 aaggtatcgg tacgttcgcc catcagttca tcaagaacct gcgcgtcacg agcggtaccc
    22981 agcgttgcag taaccagtgc ctgcgtttca ccgcgggtga acagcgcaga accgtgagta
    23041 cgcggcagca cgccagtacg cacatccaga ccacggatca tatctttttc acgaccgtcg
    23101 atacgcggtt cgcctgccag tacgcggcta cgaacaacgt ttttctcgat agcgtgcaga
    23161 atttcaccca gttcgttttc gtccagggtt tcgtcttcag caagcagcgt cgcgatggtt
    23221 tcagatttga tgacatcaac ctgcgcataa cgctcttgtt tgtcggtgat gcggtaagca
    23281 tcgctcaggc gagcttcagc cagtgcagca acgcgcgcgt tcagcgcttc gtttaccggc
    23341 tccggctgcc agtcccaacg tggtttaccg gcttctttca ccagttcatt gatgttctga
    23401 ataacaacct gctgttgttc atgaccgaac actactgcgc ccagcatctg gtcttcgctc
    23461 agcagttcag cttcagattc aaccatcaga acagcggctt cagtaccggc aacaaccaga
    23521 tccagtttgc tctctttcag ctcgtcctga gtcgggttca gtacgtactg gtcattgatg
    23581 taacctacgc gagcagcacc aatcggacca ttgaacggaa taccagacag agacagtgct
    23641 gcggaagcac caatcatcgc gacgatatcc gggttaactt gcgggttaac agaaaccacg
    23701 gtggcgataa cctgaacttc gttgacgaag ccttccggga acagcgggcg aatcgggcgg
    23761 tcaatcagac gcgcgatcag ggtttcgcct tcgcttgggc ggccttcacg acggaagaag
    23821 ctacccggga tacgaccagc agcgtaggta cgctcctgat agttaacggt cagtgggaag
    23881 aagtcctgac ctggtttggc ttttttctgg ccaacaacgg taacaaatac cgcggtgtca
    23941 tccatgctaa ccataacagc ggcagtagcc tgacgagcca tcatgccggt ttccagagtc
    24001 acggtgtgtt ggccgtactg gaatttacga acgatcggat taagcaatgt aatatccttt
    24061 ctctttctta gacagtacct tacggcactg gtgttaatac ccgatcttct gcgcatcctc
    24121 gcgactaatg acaaccctaa cccagctcta tgtgggtaaa gcctctcatt agccgcgcga
    24181 acctctgcaa cggaagatca ttcatagcaa caatacatta gtttccagtg aattgctgcc
    24241 gtcagcttga aaaaaggggc cactcaggcc cccttttctg aaactcgcaa gaattagcga
    24301 cgcagaccca ggcgctcgat gagctgggtg taacgtgcta cgtctttacg tttcaggtag
    24361 tcgagcagtt tacgacgctg agaaaccatg cgcagcagac cacgacggct gtggtgatct
    24421 tttttgtgct ctgcaaagtg gccctgcagg tggttgatct gtgcagtcag cagtgctacc
    24481 tgaacttcgg tagaaccggt gtcgtttgcg tcacgaccaa actcagaaac gattttagct
    24541 gttgcttcag tacttagaga cattttaaaa ctccaaagta tatagaatga aaggacgccg
    24601 atctctaatt cagcgatccc agtgtacgtt acgcaaagtg ttaaacaatt tacgcgacgt
    24661 taagcggcag tattctactc gtagcgacct gttatcgcaa gacggttaac attacgccgg
    24721 gtattcaacc accaggcgac gaggcgcaac gcggccttca tcgtcaattt cgcccatacc
    24781 gataaatttg ccgttctcac cttctgtaac gcgaaccagt ccttccagcg gcgcaccgga
    24841 tgtacgaact gggttaccat ttttgaagta aacagaagac gttaacggaa gattcaccac
    24901 cgggtagtcc gaagctggac tgtccattgg catcagtaat ggatcaagta actccgcagc
    24961 cggaatatcc tgctgttcgg cttgttcgac cagttcacgc agatgttcca gggtcaccat
    25021 ccgttcaacc ggatatttac ttaccgccag acgacgcagg taaataacat gcgcgccaca
    25081 accgagtttt tcacccaggt catcaatgat ggtgcggata taagtgcctt ttgagcagtg
    25141 aatttccagc tccagctcat tgccttcgtg gcgaataaac agcaattcat aaacggtaat
    25201 cgggcgcgct tcacgcggta cttcaatgcc ctgacgcgca tattcgtaca gttttttacc
    25261 ctgatatttc agtgctgaat acatcgaagg gatctgttcg atatcgccac ggaaagtatc
    25321 cagcgccgcc gccagttgct ctgcgctaaa ggttaccgga cgttcttcaa cgatttgtcc
    25381 gtcggcatca gaagtatcgg tacgctgtcc aagacgcgca atgacccgat agcgtttgtc
    25441 ggagtccagc agatactggg aaaacttcgt cgcttccccg aggcaaattg gcaacatgcc
    25501 agttgccagc gggtccagcg caccggtatg cccggcacgg ttggcgttat aaatccgttt
    25561 cactttttgc agcgcatcat tgctggacat accctgcggt ttatccagca acaaaacgcc
    25621 gttaatgtcg cgaccgcgac gacgaggacg actcattagt cctccttgct gtcgtccggg
    25681 ttaacacgac gttcttcgtc atgtttgacc acgctggtca ccaggtttga catgcgcatc
    25741 ccttcaacca gagagttgtc gtagaagaag gtcagttccg gcacgatacg caggcgcatc
    25801 gctttcccca gcaggctgcg gatgaaacca gaagcttctt gcaacgcttt gatgcccgct
    25861 ttaaccgcgt cttcatcttt gtcgttgagg aacgtcacat atactttggc atacgccagg
    25921 tcgcgagaca tttcgacacc ggaaacggtg gtcatcatgc ccaggcgagg atctttaatt
    25981 tcacgctgca tgatgagagc gatctctttt tgcatttcct gcgctacgcg ctgcgggcga
    26041 ccaaattctt tcgccataat aaattctcct gacaaaaaag gggctgttag ccccttttta
    26101 aaattaattt caggtggaag ggctgttcac gttggcctga taagacgcgc cagcgtcgca
    26161 tcaggcaatc catgcaggat gtggcgtaaa cgccttatcc cgcatggaac cctaaaaacc
    26221 ttaagcgatg gtacgttgga tttcgatgat ttcgaatact tcgatcacat cgccagtgcg
    26281 gacgtcgttg tagttcttaa cgccgatacc acattccata ccgttacgga cttcgttaac
    26341 gtcatctttg aagcggcgca gggactccag ctcgccttcg tagataacca cgttgtcacg
    26401 cagaacgcgg atcgggttgt gacgtttaac cacaccttcg gtaaccatac agcctgcgat
    26461 ggcaccaaat ttcggtgatt tgaacacgtc acgaacttcc gccagaccga taatctgctg
    26521 tttcagttcc ggagacagca taccgctcat cgccgctttc acttcgtcaa tcaggttata
    26581 gatgacggag tagtaacgca gatccaggct ttccgcttca atcactttac gtgcagaggc
    26641 atcagcacgt acgttgaagc caaccaggat ggcgttggac gccgcagcca gggtggcgtc
    26701 ggtttcggtg ataccaccta cgccagaacc gatgatcttc actttaactt cgtcagtaga
    26761 cagtttcagc aaggagtcgg agatcgcttc cacagaaccc tgtacgtctg ccttcaggac
    26821 gatgttcact tcgtgaactt cgccttcggt catgttggcg aacatgttct cgagtttaga
    26881 tttctgctga cgcgccagtt taacttcgcg gaatttaccc tgacgataca gtgcaacttc
    26941 acgcgctttc ttctcgtcac gtacaacggt aacttcatca cccgcagccg gtacgccgga
    27001 caggccgagg atttccaccg gaatggacgg acccgcttcc agcacttcct gacccagttc
    27061 gttacgcatc gcacgaacac gaccgtattc gaagccacac agaacgatat cgcccttgtg
    27121 cagagtacct tcacgtacca gaacggtagc aaccggacca cgacctttat cgaggaagga
    27181 ttcgataacc gcaccgctcg ccataccttt acgtaccgct ttcagctcca gaacttccgc
    27241 ctgcagcagg atagcgtcca gcagttcatc gataccagta cccgctttcg cagacacatg
    27301 tacgaactgg ctttcaccgc cccactcttc cggcaggatg ccgtactggg agagttcgtt
    27361 cttaacgcga tccggatcgg cttctggttt atcgatcttg ttcactgcaa cgaccaccgg
    27421 aacctgcgcc gctttcgcgt gctggattgc ttcgatcgtc tgcggcatta cgccgtcgtc
    27481 ggcagcaaca accaggacta cgatgtccgt tgcctgcgca ccacgagcac gcattgaagt
    27541 aaacgcggcg tgccctgggg tgtccaggaa ggtgatcatg ccgttttcag tttcaacgtg
    27601 gtatgcacca atgtgctggg taatgccgcc cgcttcgcca gaggccactt tcgttgaacg
    27661 aatgtagtcc agcagagagg tcttaccgtg gtcaacgtga cccatgatgg tcacaaccgg
    27721 cgcgcgcggt tcagccgcag cacccgtgtc acggtcgctc attaccgcct cttccagctc
    27781 gttttcacga cgcaggataa ctttatggcc catctcttca gcaaccagct gtgcggtttc
    27841 ctgatcgata acctggttga tggttgccat tgcgcccagt ttcatcatcg ctttgatgac
    27901 ctgagagcct ttaaccgcca tcttgttcgc cagttcgcca acggtgatag tttcgccgat
    27961 cacaacgtca cggttaacgg cctgagcagg cttctggaag ccttgctgca gggaagaacc
    28021 tttacgtttt ccgcctttac cgccacgtac tgctgcgcgt gcttcttcac gatcagcttt
    28081 tgattcagcg tgtttgttac ctttcttcgg acgcgctgct ttcgcgttac gaccacggcc
    28141 acggccgcct tcgacttcac gatcgctttc gtcttcggcc tggcgagcat gttgagaagt
    28201 agtgacgtga taatcgctgg aatcttcagt cggttccgcg ttatcagtcc atttgttttc
    28261 ttccgccata cgacgtgctt cttcagcaac gcgacgtgct tcttcttcga gtttacgacg
    28321 cgcttcttct tcagctttac gcttgagctc tgcagcttcc tgctcacggc gggctttttc
    28381 agcctgggcg tttttagtca tatcgtcttg ttgattgctc actttgtctt tttccgcagc
    28441 ttcacgtttc gcttgttcag cagcttcacg cttagcttgt tctgcggcct cacgttcagc
    28501 tttttgttgc gcctcgcgtt tagccgattc ttctgcctca cgacgggctt gctcttccgc
    28561 ttcacgctgc gcttgctctt ccgctgcaag gcgttcagcc tcttgcggat cgcgtttcac
    28621 aaaggtgcgt ttcttgcgga cttcgatttg taccgatttg ctttttccac cggtaccagg
    28681 aatgttaagg gtgctgcgtg ttttacgctg cagcgtcaat ttgtccgggc ctgaattttt
    28741 ctgattcagg tggtcaatca aagtctgttt ctcttgtgca gacacagagt cgtcagcaga
    28801 cttccggata cctgcatcag caaattgctg taccaggcgt tccacggagg tctgtcgctc
    28861 tgcggccagc gttttaatcg ttacatctgt catgctgttc cttcctgcta cagtttatta
    28921 cgcttcgtca ccgaaccagc aaatattacg ggcagccata atcagtgctc cggctttttc
    28981 gtcggtcaac ccttcgatat cagccagatc atcaatgccc tgttcggcga gatcttccag
    29041 cgtacaaacg ccacgggcgg ccagtttgaa tgccaaatca cgatctaccc cttcaaggtt
    29101 cagcagatcg tcagccggtt tgttatcacc gaggctttct tcctgggcct gtgcaatggt
    29161 ggccagtgca tttttagcac gctcgcgcag tgcttcaacg gtcggctcat caaggccttc
    29221 gatttccaac agctctttca tcggcacata ggccagttct tccagcgtcg agaagccttc
    29281 ttctaccaga acagtcgcga agtcttcgtc gatgtcgaga tatttggtga aggtgtcgat
    29341 cgctgcgtgc gcttccgcct gatgcttagc ctgcaggtcg tcaacggtca tcacgttgag
    29401 ttcccaaccg ctcagctgcg aagccagacg cacgttctga ccgttacggc caatcgcctg
    29461 cgccaggtta ccggcttcaa cggcgatatc catggtgtgt ttatcttcat ccaccacgat
    29521 agaagcaacg tctgccggtg ccattgcgtt aatcacaaac tgcgccgggt tatcatccca
    29581 caggacgata tcgatacgct cgccgcccag ttcagtagaa accgcctgaa cacgcgcgcc
    29641 acgcatacct acacaggcac ctaccggatc gatacgttta tcgttggttt tcaccgcgat
    29701 tttcgcacga gaacccggat cgcgagccgc tgctttaatt tcaatcactt cttcgccgat
    29761 ttctggcact tcaatacgga acagttcgat cagcatttcc ggcttggaac gagtgacgaa
    29821 cagttgcgcg ccacgcgctt ccgggcgaac ggaatagagc acgccacgaa cgcggtcgcc
    29881 agggcggaag ttttcacgcg gcagcatatc ttcgcgcagg atcacggctt cagcattgtt
    29941 gcccagatcc agagagatgt tgtcgcggtt tacttttttc accacgccgg tgatgatttc
    30001 accttcgtgt tcacggaact gatcaaccac catcgcacgt tcggcttcac gcactttctg
    30061 cacgataacc tgttttgccg tctgggtagt gatacggtca aaggtaacag actcaatctg
    30121 atcttcaacg taatcgccca ggttcaggct ttcatcttca taacgtgcgg cttcaagggt
    30181 gatttccttg gtcggctggg tgacttcatc aacaactaac cagcgacgga aggtgtcaaa
    30241 atcaccgctt ttgcgatcga tctgtacgcg gacgtcgatc tcttgttcat attttttctt
    30301 tgttgctgtc gccagcgcgc tttccaatgc ttcgaaaatc ttctcgcgag gtagcgcctt
    30361 ttcattggat acggcttcaa ctacagccaa aatttctttg ttcatcgcgg gcttttcacc
    30421 tcatccagac tattaaaagt ggggaaccag gttcgccttc tggatattac tcagcgcgaa
    30481 cacttcatct ttaccttcga cggtaactgt gatcatttca ccgtctaccg ctttgataac
    30541 gccctgccat ttacgacggt tttgtaccgc catacggaga accagagtca cctcttctcc
    30601 gacaaaacgg gcgtagtgtt cagccgtgaa cagtgggcga tcgagacccg gtgaggagac
    30661 ttccaggtta taagcaacgg tgatgggatc ttcaacatcc agcacagcac ttacctggtg
    30721 gctcacatca gcacaatcat caacattgat gccatcttca ctatcaatat agatgcgcag
    30781 tgtggatgtg cgaccgcgaa taaattcgat gccaaccagt tcaaaaccca gggcctcaac
    30841 tggcgcagta atcatctctg ttaatttttg ctctaatgtg gacaagccca cccccaagac
    30901 ataaaaaaag ggcctaaagc ccagttattc tgtagtcaga taacaaaaaa ccccgataaa
    30961 tcggggcttt atataactga accctataac cgcaactgcg gtctggagca ctttccagaa
    31021 ggattttttc aaatcccact acgaaggccg aagtcttcac agtatatttg aaaaaggact
    31081 ctaagggaaa gtggttgcgg gggccggatt tgaaccgacg accttcgggt tatgagcccg
    31141 acgagctacc aggctgctcc accccgcgcc tgaaacgtgg caaattctac tcgttttggg
    31201 taaaaaatgc aaatactgct gggatttggt gtaccgagac gggacgtaaa atctgcaggc
    31261 attatagtga tccatgccac attttgtcaa cgtttattgc taatcacgtg aatgaatatc
    31321 cagttcactt tcatttgttg aatacttttg ccttcccctg ctctccctta agcgcattat
    31381 tttacaaaaa acacactaaa ctcttcctgt ctccgataaa agatgattaa atgaaaactc
    31441 atttattttg cataaaaatt cagtgagagc ggaaatccag gctcatcatc agttaattaa
    31501 gcagggtgtt attttatgac gacgattctc aagcatctcc cggtaggtca acgtattggt
    31561 atcgcttttt ccggcggtct ggacaccagt gccgcactgc tgtggatgcg acaaaaggga
    31621 gcggttcctt atgcatatac tgcaaacctg ggccagccag acgaagagga ttatgatgcg
    31681 atccctcgtc gtgccatgga atacggcgcg gagaacgcac gtctgatcga ctgccgcaaa
    31741 caactggtgg ccgaaggtat tgccgctatt cagtgtggcg catttcataa caccaccggc
    31801 ggcctgacct atttcaacac gacgccgctg ggccgcgccg tgactggtac catgctggtt
    31861 gctgcgatga aagaagatgg cgtgaatatc tggggtgacg gtagcaccta taaaggaaac
    31921 gatatcgaac gtttctatcg ttatggtctg ctgaccaatg ctgaactgca gatttacaaa
    31981 ccgtggcttg atactgactt tattgatgaa ctgggcggcc gtcatgagat gtctgaattt
    32041 atgattgcct gcggtttcga ctacaaaatg tctgtcgaaa aagcctactc cacagactcc
    32101 aacatgcttg gtgcaacgca tgaagcgaag gatctggaat acctcaactc cagcgtcaaa
    32161 atcgtcaacc caattatggg cgtgaaattc tgggatgaga gcgtgaagat cccggcagaa
    32221 gaagtcacag tacgctttga gcaaggtcat ccggtagcgc tgaacggtaa aacctttagc
    32281 gatgacgtag aaatgatgct ggaagctaac cgcatcggcg gtcgtcacgg cctgggcatg
    32341 agcgaccaga ttgaaaaccg tatcatcgaa gcgaaaagcc gtggtattta cgaagctccg
    32401 gggatggcgc tgctgcacat tgcgtatgaa cgtctgttga ccggtattca caatgaagac
    32461 accattgagc agtatcacgc acatggtcgt cagttgggcc gtctgctgta ccaggggcgt
    32521 tggtttgact cccaggcgct gatgctgcgt gactctctgc aacgctgggt tgccagccag
    32581 atcactggtg aagttaccct ggagctgcgc cgcgggaacg attattcaat cctgaatacc
    32641 gtctcagaga acctgaccta caagccagaa cgtctgacga tggaaaaagg cgactcggtg
    32701 ttctcgccag atgatcgtat tggtcaattg accatgcgca acctggatat caccgatacc
    32761 cgcgagaaac ttttcggtta tgccaaaact ggcctgctct cctcctctgc cacttcaggc
    32821 gtgccgcagg tggagaatct ggaaaacaaa ggccagtaat tcgacatcaa ccctgccctt
    32881 caggggtggg gttaccttgc ttatcctctg taaactcctt tctgagcatc acataatcga
    32941 aaacatcatg gtttgcatcc acaacccgtg agtctccttt atagtgtgca atcacttcct
    33001 caagcgtttg cggctgttcc ggctttgtta cccccatcca cgcattaata aggtaattat
    33061 tgtaagctgt cgaaaagatg ttgttttccg ttgtgcgatc cacgccatcc ccaagaacgg
    33121 ggctatacca gataaacatc gggacatgat atgcctgctg gctagcctcc ctgcctccat
    33181 gaaaatagac gttcttcttc gtagggtcac gttccaggcc gtggtcggca aaatacatga
    33241 ccgaggcgcg gcgatctttt aataattcaa aaacctgacc tagcaaacta tctgtgtaat
    33301 gaatggagtt gtcatagcag gcatcctgat cgtcctgcgg ttgaaacacg gcgctggatt
    33361 gcggataggc gctacaagcc ggttcatggc ttccatttaa atgaagaaca atcagtttct
    33421 tttgctgcgt attttgctgt aatgcttgcg ataaatgcgg caacaacaat tcatcaaatc
    33481 ctctgacata aactgtttcc atggcgcgca tggcgatact ggtaactgct gtaccattct
    33541 gccgaaaagc ggattgcgag cttagccaga aagtctgaaa tcctgcttga ttagccatat
    33601 taataatgtt gtcggggtaa ttatgaatgt catgactcaa aacagaatca gcagttaaag
    33661 aaaggggaac cgacagcgca gtgtaaggtg cgccgcttat tgcttgatta aacagtttga
    33721 tctgttttct ttgtgcttca acttgcggtg tcgtagagcg tgtatatcca tacaaagaca
    33781 tattgtcgac acgtacagac tcccccacaa tcaacacgta ggtatcaata cctgtatccc
    33841 tgactgataa ttgaaaatac ggcaccgtgt ttgcaattga gagtaatctt tgatgctctt
    33901 tcgctgctaa agcaaaatag ttgagattga aaaatggcgt ataggtagca aatcgcgacg
    33961 ctagtatata tggactgaac gcttttttat tttttgcatc tttataagca aattgacaag
    34021 cggaaaataa actgcccgaa atgacaatca gcaataatat tcctgtcact ttttttgtcg
    34081 gcaaggaaac atcatatttt attattactg ccaaaaaaag aagggataaa aaagcaaagg
    34141 cacatagata agggatatac atccccagca ttttgacaac ttcatccgga tcactctgga
    34201 gcacactaat cgcaaatcca tcattgaaag ttgtaccaaa ggtacaccat gaatagaggc
    34261 taatactcat gtctgccgca gtcagtaaca caaatggtgc ggcaacaagc aggcgaagcg
    34321 gaagggatga tatacgtttt acaaccagga tggtcaggac gaagaacagc aatcgctgaa
    34381 ccatatgcgc gccggaagag gcgactaagt tcgtaatacc aaaaagaata acacaaagat
    34441 acaacaacca atgagattta aaacttctag caaatttgtt gaatactgtc atccagaaat
    34501 ccaagttcac aaccaatata aacaattatc aacaatgttc aattttatat cagacatgtt
    34561 tttcaactct gatccatatc agaccgaaac actgttagtt ttttcttttt aaatgatgcg
    34621 gccattgcgt tctcagtaat taaataacat tatcaatgcg tctctaaaca ttacgcagaa
    34681 cgtagccatc agtagttata ctgacaggag caggatggtt tatcgagaag taaacatatc
    34741 cgtattcgta ccggaactgg agttgacaca ataaagtgcc aattatgtca gcagaaggga
    34801 aaagtttata actaaggcgt accggcacca tcgtttcaag gtaccagcta cgagtaaagc
    34861 aactggacga gatacagata cctgacaacc attcctcaga ccaggaccaa aacgaaaaaa
    34921 gacgcttttc agcgtctctt ttctggaatt tggtaccgag gacgggactt gaacccgtaa
    34981 gccctattgg gcactaccac ctcaaggtag cgtgtctacc aattccacca cctcggcacg
    35041 gatactactt tttagttcgg gatatcgctg gtcggcttag ccggagcagc tggttgagtt
    35101 tgttcggttt tcaccggtgc actcagattt tcccattcgc tacctttatt ggttttgttg
    35161 ctattgatgt tacccagcac cagactgatg atgaagaata acgttgccag cagcgccgtc
    35221 atgcgggtca tgaagttacc agaaccactt gaaccaaaca gcgtagcgga agcgcctgct
    35281 ccgaaggagg ctcccatatc agcgccttta ccttgctgca gcatgatcag accaacaagg
    35341 ccaattgcca caataaggaa aactactaaa agagcttcgt acataatcaa cctgttcctt
    35401 gcggagttgc cgcgtaccaa tgcttcaacc aataaagcgg gagtttttaa tctttcccac
    35461 tgaagcgggt gtgaatacta accaaagcga atgaccttcg caagggcaat tttatcgcat
    35521 tgtatcaact gcggaaaaaa acagcaaaac cgatgtgttc gctgagaaaa aaggcgacaa
    35581 tcgccgcctt tttagccagt tatctaacgc tttaaacggc ttttactgca tcggcgatgc
    35641 ggtgtgcaaa ttcagtcacc tgcgcttcgt cttcgccttc caccatcacg cgaattaacg
    35701 gttcggtgcc ggatttacgc agcaacacgc gtccacggtt gcccagcgca gcttcaacct
    35761 ctgcggtcac ggctttaact gactcatgct caagtggatc gccgctacct gcggtgtaac
    35821 gtacgttaac aagaatctgc gggaacattt tcatgccgct gcaaaggtcg tgcaggctca
    35881 tatggttacg tgccatcgcc gccagcacct gcaagccagc aacgatgccg tcaccggtag
    35941 tagttttatc cagcaggatc acatgaccgg aattctctgc accgatacgc cagcctttct
    36001 cctgcatttt ttccagtacg tagcggtcac ccactttcgc gcgcgcaaat ggaattccta
    36061 actgtttcag cgccagttca agccccatgt tgctcatcaa tgtacccaca gcgccaccac
    36121 gcagctggcc ctgacgaaga ccttcacgcg cgatgatata catgatctga tcgccatcga
    36181 ctttattgcc ttcatggtca accataatca cgcgatcgcc atcgccgtcg aaggcaatac
    36241 cgagatccgc tttttcagcc agcacacgag cctggagcgc gcgaacgtcg gtagccccca
    36301 cttcggcatt gatgtttaca ccgtttggct cacaaccgat agcgataacg ttcgccccca
    36361 gttcgcgcag cacgttcggc gcgatgtgat aagtcgcacc gtttgcacaa tccaccacaa
    36421 tcttcagttc actgaggcta agttcgttcg ggaacgtggc tttgcaaaac tcgatatagc
    36481 gacccgcggc atcaacgata cggctggctt tacccagttc tgccgaatca acgcagctga
    36541 tctccttttc catttccgct tcgatggcct cttctaccgc atccggcagt ttggtgccgt
    36601 cgatagagaa gaatttaatg ccattatcgt agaacgggtt atgcgatgca gatatcacaa
    36661 ttccggcctc tgcgcggaag gtacgcgtca gataagccac ggccggtgtt ggcatcgggc
    36721 cagtgaagag tgcggaaagg cccgctgccg ccagacccgc ttccagtgct gactccagca
    36781 tatagccaga aatacgcgtg tctttaccaa taataatctt acgggagccg tggcgcgcca
    36841 gcactttacc cgcggcccaa cccagcttaa gcacaaaatc aggtgtgatc ggcgcatccc
    36901 ctacacgacc acgaatccca tcggtaccga aatatttacg attactcata gcgttagttt
    36961 tcctttgcag acagagtggc ttccaccacc cgcatcgctt ctacggtttc tttgacgtca
    37021 tgaacacgaa tgatgtgcgc gccttgcatt gcggcaatga ccgcacaggc cagactaccg
    37081 ctcaggcgct cggacggccc cacgttcagc agctgcccaa tcatcgattt tcgtgacata
    37141 cccaccaaca gcggcaggtt gaaatggtga aattcagcca ggcgcgccag taatgaatag
    37201 ttatgggaga gatttttacc gaaaccgaat ccggggtcga gcaacaattt ctcttttgcg
    37261 atacccgcct gctcgcaacg tgctatttgc tcaataaagt agcgattcac ttctgcaaag
    37321 acatcgtcat acttcggagc ttcctgcatg gtttttggat ttccctgcat atgcatcaga
    37381 caaaccggta aaccggtttc tgcagccgcc tccagagcgc caggttcgga aagggagcgg
    37441 atatcattaa taatgtgagc gccaactttc gctgactcac ggatgacttc tggtttggat
    37501 gtatcgaccg agatccagac ttcgaagcgt tgagcaattg cctcaaccac aggaataaca
    37561 cgttgcaact cttcttcaac gctaacttcc gccgcccctg ggcgcgtgga ctcgccacca
    37621 acgtcaatga tcgtcgcgcc agcattgatc atcagattcg catgtttcac cgcatctatc
    37681 agcgagttat gcgtgccacc atccgaaaag gaatcaggcg tgacgttgag gatccccatt
    37741 acgtgaggat ggctaaggtc cagtgaagta ccctgggcaa agagtttcat gatgttatcc
    37801 ctggtatgaa ttgataagaa aaaaccccgg agcacgcccc ggggttttcg gtacaaatac
    37861 agtcatctga tgcgggaact tacttgtcgc ctaactgctc tgacatggtg ttacccgggt
    37921 tcggcgtacg cggttcatca accggacgag gagcctttgg actaccattg tcgccagaat
    37981 tgttagaagc gcctggttct tcccagcccg ctggcggacg tacatcgcga cgtgccatca
    38041 ggtcatcaat ctgcggtgcg tcgatagtct catatttcat gagagcatct ttcatcgcat
    38101 gcagaatatc catattgtcg gtcagaagct gacgcgcacg attatagtta cgctcaatca
    38161 gtgctttcac ttcctggtcg atgatacgtg cagtttcatc ggacatatgt ttcgctttcg
    38221 ctacgctacg gccgaggaat acttcacctt cttcttccgc gtacagcagt ggacccaatt
    38281 tctcagagaa gccccactgg gtcaccatgt tacgtgccag gttggtcgca actttaatat
    38341 cgttggacgc accggtagaa acatgttccg gcccgtagat gatctcttct gccagacgac
    38401 caccgtacag cgtagaaatc tggctttcca gtttctgacg gctggcgctg attgcgtcgc
    38461 cctcaggcaa gaagaaagtc acacccagcg cacgaccgcg tgggataatc gtcactttgt
    38521 gcaccggatc gtgttccggc accaggcgac cgataatcgc atgacccgct tcgtggtaag
    38581 ccgttgattc tttctgcgct tccgtcatca ccatggagcg acgttccgca cccatcatga
    38641 ttttgtcttt cgctttctcg aactcaacca tcgacacaac gcgtttgttg ccacgagcag
    38701 cgaacagtgc cgcttcgttc accaggttcg ccaggtcagc accggagaaa ccaggagtac
    38761 cacgggcaat gattgccgcg tcgatatcgg gggccaatgg tacgcgacgc atgtgaactt
    38821 tcaggatctg ctcacgaccg cgaacatctg gcaagccgac cacaacctga cggtcgaaac
    38881 ggccaggacg cagcagggcc gggtcgagaa cgtccggacg gttagtcgcg gcgataacga
    38941 tgataccttc gttaccttcg aagccatcca tctcaaccag catctggttc agagtctgtt
    39001 cacgttcatc gtgaccaccg cccagaccag cgccacgctg gcggcctacg gcgtcgattt
    39061 catcgataaa gatgatgcac ggtgccgctt tcttcgcctg ttcgaacatg tcacgaacac
    39121 gggatgcacc cacaccgacg aacatttcta cgaagtcaga accggagata gtaaagaacg
    39181 gaactttcgc ttcgcctgca atcgctttcg ccagcaacgt tttaccggta cccggaggac
    39241 cgaccatcag gacgcctttc gggatcttac cgccgagttt ctggaagcgg ctcggctcgc
    39301 gcagatactc taccagttca gcaacttctt cttttgcttc gtcgcagccc gcaacgtcag
    39361 caaaggtcgt tttgatctga tcttccgtca gcatgcgcgc tttgctctta ccaaacgaca
    39421 tggcaccttt gccaccgccg ccctgcattt gacgcatgaa gaagatccag acaccaatca
    39481 gcaacagcat cgggaaccag gagatgaaga tagaagccag caggcttggt tcttcaggcg
    39541 gttcaccgac aaccttgacg ttcttggtca acaggttatc cagtaatttc ggatcctgaa
    39601 ccggaatgta agtggtataa cggttactat ctttcttggt aacgttgatt tcacgtccgt
    39661 tgatacgcgc ttcacgaacc tggtcgttat tgacctcttg taggaaggta gagtaatcca
    39721 ccttacggcc attagactcg ctgggcccaa agctctggaa tactgacatc agcacaacgg
    39781 caatgaccag ccagagtatt aggtttttcg ccatgtcact caagggatta acctcttatt
    39841 acaactgtgt taaaaacagc gtcaggatac tctatatcca gcatctttca aactttcgtc
    39901 tgaaatctcc cggttagggt ttacgcccgg tcgctacaat atacacttcc cgcgaacgtg
    39961 cacgagaaga gtccggctta cgaactttga ccttcgtaaa cagggagcga atttccctta
    40021 gatactcatc gaaaccttcg ccctggaaca ccttcactac aaaactgcca cctggcgcta
    40081 atacatcacg acacatttct agcgccagtt ccaccagata catggcacgg gggatatcca
    40141 ccgccggtgt tccgctcatg tttggtgcca tatcggacat gacaacctgg actttgctgt
    40201 cgccaacgcg ctccagcagt gctttcatca ccagttcatc acgaaaatcg ccctgaagaa
    40261 agtccacacc aacgatagga tccataggta aaagatcgca agcgatgatg cggcctttgc
    40321 cgccaatttg ggtgaccaca tattgtgacc aaccacccgg agcagcacca aggtcgacaa
    40381 ccgtcattcc cggtttaaag agtttgtcac tttgctgtat ttcatcaagt ttaaaccagg
    40441 cacgggaacg taaccccttt ttctgtgcct gttgaacata tttatcgcta aagtgttcct
    40501 gaagccagcg gctggagctg gcagaacgct tcttacctgt catttaactt tcccatgggg
    40561 ataactcatc gtaaccaatt gcgtaaattt ttacgcgcct atttggtgat atatgggaga
    40621 tggcggtaga atgacccgtt ttcaatccca acgtaagcaa aaaatacgat gaatctgagt
    40681 actaaacaaa aacagcacct gaaaggtctg gcacatccgc tcaagccagt tgttctgctt
    40741 ggcagtaatg gtttgaccga aggggtgctg gccgagattg aacaagcgtt agagcaccat
    40801 gaacttatca aggtgaaaat cgccaccgaa gatcgcgaaa ctaaaacctt gatcgtggaa
    40861 gctatcgtgc gcgaaaccgg cgcctgtaat gtacaggtca tcggtaaaac gctggtgctt
    40921 tatcgcccaa ctaaagaacg taaaatctcg ctgccacgct aagattatcc taaagttaca
    40981 cacatttgct gtgtaaaacg aggggttttc cgcaggcagg agagcaaaat gccacgctct
    41041 gttcgttgat aaaaggccgc atagcggcct ttttcctttc tttacaatac atcaacatct
    41101 tgagtattgg gtaattctta caggtattcc accttaatta cttcaaattc tacttcgccg
    41161 cccggcgttt tgatgaccac aacatcatct tcttctttgc cgatcaggcc acgagcaata
    41221 ggtgagttta cagaaatcag gttttgttta aagtcagctt cgtcatcgcc aacgatgcga
    41281 taagtctgtt cttcgtcaga atccagattc agcaccgtta cggtagcacc aaaaataacg
    41341 cgcccattgt tgggcatttt ggtgacatca atcacctgcg cgttcgacag cttggcttcg
    41401 atgtctttga tacggccttc gcagaagccc tgctgttcac gagctgcgtg gtattcggcg
    41461 ttttctttca ggtcgccatg ctcacgcgct tccgcgatag cagcaatgat ttcaggacgg
    41521 cgcacagatt tcagaaaatc cagctcttcg cgtaattttt cagcgccgcg taaggtcatc
    41581 ggaatagctt gcatttgtta tacctcttga atattcctga tggggcaagt cttcacccac
    41641 cccggctgtt cggcctgccc ggcataactc ctcaccggga ccagaagcaa aaaaataccg
    41701 acccgggtac aagtcccagg tcagctacaa ttcacatttt gatagtcatt ttaccctgaa
    41761 gttcccgaag ggtcatcgtt tactttatag ggcgttgcgc cgtagtatga cggctcgatt
    41821 ccaggttgtt agcgcgagat tatgcgattt tccagattta tcatcggatt gaccagctgt
    41881 atagcgttca gtgttcaggc cgcgaatgtt gatgagtacg ttactcaact ccccgctggt
    41941 gccaaccttg ccctgatggt gcaaaaagtc ggcgcgtcgg cccccgctat tgattaccac
    42001 agtcagcaga tggcgctgcc cgccagtacg cagaaagtga ttactgcgct ggcggcgttg
    42061 attcaactcg gccccgattt tcgttttacc acgacgcttg aaaccaaagg caatgtggaa
    42121 aacggcgtac ttaagggtga cttagtggcg cgatttggtg ccgatccgac gttaaaacgt
    42181 caggatattc gcaatatggt cgcgactttg aaaaaatctg gcgtcaacca aatcgatggc
    42241 aatgtgttga tagatacctc cattttcgcc agccacgata aagcccccgg ctggccatgg
    42301 aatgacatga cacaatgctt tagcgctccg cctgccgccg ccatagttga ccgcaactgt
    42361 ttctccgtct cgctctacag tgcccaaaaa cccggtgata tggcctttat acgcgtggca
    42421 tcttattacc ccgttacgat gttcagccag gtacgcaccc tcccccgtgg ttctgccgaa
    42481 gcgcaatact gcgaactgga tgtggtgcca ggcgacctga accgctttac gctgacggga
    42541 tgcctgccac aacgttctga gccgctcccg ttggcttttg ccgtacagga tggagccagc
    42601 tatgccgggg caattctgaa agatgagtta aaacaggcgg gtatcacctg gagtggaaca
    42661 ctgctgcgcc agactcaggt taacgaacct ggaacggtag ttgccagtaa acagtcggcc
    42721 ccgctgcacg atctgcttaa gattatgctg aaaaagtcag acaacatgat cgccgatacg
    42781 gttttccgca tgataggcca tgcgcgcttc aatgtgcctg gaacatggcg ggccgggtcg
    42841 gacgccgtgc gtcagatcct gcgccagcaa gccggtgtcg atattggaaa caccattatt
    42901 gccgatggtt cagggctttc gcggcataac ctgattgccc ccgccaccat gatgcaggtg
    42961 ctgcaataca ttgcccaaca cgacaatgaa cttaacttta tctccatgct gccgctggcg
    43021 ggctatgacg gctctttgca gtaccgtgca ggtctgcatc aggcgggcgt ggatggcaaa
    43081 gtctcagcga aaaccggttc gttgcagggg gtatataacc tggcgggatt cattaccaca
    43141 gcgagcgggc aacgaatggc gtttgtgcaa tatctttctg gctatgcagt agaaccggcg
    43201 gatcagcgta atcgccgtat tccgttagtg cgtttcgaaa gccgtttgta taaagatatt
    43261 taccagaaca attagtcaaa aagaaacccc ggcatatggc cggggtttca gattattgac
    43321 aaagtgcgct ttgtttatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcgt
    43381 gcaaattcaa tatattgcaa tcatcttgta ggcctgataa gtgtagcgca tcaggcttat
    43441 ttggcattgg tcattagtga ttaacgcttg taaatgaact caacgccttc ttcgtcgtct
    43501 tcgtcccagt cgtcatccca gtcctcatca tcctcttcag caatctcttc aagctgctgg
    43561 cgatgataat catcccacat gaattcgact ttctctggct gtttcgcttc ttcagcctgc
    43621 acgaccgggt tttcaatgat aaaggtcatc acatcccagc agagatcttt cacgcccagt
    43681 ccgctcgccg cagagatcag ataatattta tcttcccagc ccagcgcctc agcgatcgct
    43741 ttcgctttct cttcggcttc tgccttatcc agcagatcga tcttgttgaa cactaaccaa
    43801 cgcggtttcg ccgccagatc ctggctgtat ttttccagct cgctgataat aatacgcgcg
    43861 ttttcaaccg gatcggtgcc gtcaatcgga tcgatatcga tgaggtgcaa caggacgcgg
    43921 caacgttcca ggtgcttcag gaagcggata cccagacctg cgccttccgc agcgccttca
    43981 atcagtcctg gaatatcggc aacaacgaag ctcttttcgt tgtccattcg taccacaccc
    44041 agacttggca ccagagtggt aaacggataa tccgccactt tcggtttagc cgccgatacc
    44101 gcgcgaataa aggtcgattt ccccgcgttt ggcatcccca acataccgac gtcagccagc
    44161 agcatcagct ccagcagcag ctcacgctta tcacccggcg taccgttggt tttctgccgt
    44221 ggtgtacggt taacggacga tttgaaacgg gtattgccca gaccgtgcca gccgccctta
    44281 gcaaccagca gacgctgacc gtgtttagtc atatcgccca tggtttcacc agtaccctgg
    44341 tcgattacac gcgtacctac cggcacttta atcgtgacgt ctttaccgcg cttaccggta
    44401 cagtcgcggc ttgcgccgtt ttgaccgcgc tctgcacgga aagatttttc aaaacgataa
    44461 tcgataagcg tgttcaggtt ctcgtcggct tccatccata cgtcaccacc atcaccgccg
    44521 tcgccgccat ccgggccgcc tttcggaata tacttttcgc ggcggaagct cacgcaacca
    44581 ttaccgccat cacctgcaac gaccagaatc gatgcttcat caacaaactt cattttattc
    44641 tccgtaaatc attcgcctgc gcggggttgc gataccaccg ttgtatgctt acgtaatccg
    44701 ccccaaatac gatgaccaat ggcggaatac atcgcgcccg caaccacgac aaacgcaccg
    44761 agataaccta aaaggtttaa catcggtctg gcgaagaaat cgggccaggc cagtgataaa
    44821 agatctgaaa aaaacagcgt aaacagtggg gtgagcgtga tgatcgcgct cacctgcgct
    44881 gcctgccagc gagccatcgc ttccgccagg gcgccatatc ctaccaatgt attcagtccg
    44941 caaaaaatta aacatgcgag ctgccagtgg ctaagctgcg ctatcactcc aggctttgcc
    45001 agagggaaga gcgcgattgt acataaagtg tacagtaaaa acagaatctg cggtgaggcc
    45061 agccgacgca ataaaacctt ttgcgccacg ccataactca cccaaaccgt cgccgcaccg
    45121 accccaaaga taactcccca ggtgtaatcg gtgagctttg taaatatctc gaccagactg
    45181 gtgttaaaaa acatcaccag gccgctcagg agcatcaatg cccctacaac ctgagtgctg
    45241 cgcattttct ctttcaggat aaatacgctg gcaaccatca tgccaactgg cgagagttgc
    45301 ccaatcacct gcgaagcggt cggactcagg tattgcaagg atgagctgaa caggatgaag
    45361 ttcccaaaca gcccggcggt cgccactgcc aacaaaatca accagcgtgg cttacgaaac
    45421 acgcgtaatg gcggcaacct cttcttcacc gcaagaatgg caccaaggcc aatactcgcc
    45481 atcaagaaac ggtaaaacac gattgtcgga ggttccatca cctccagcac ctgcttcatt
    45541 gcgattggca acgcccccca gcaaattgct gtggtgagcg ccaaaagaat gccaatgcct
    45601 gcctgctgct tcataccgta ttccctgcaa agcgcatttg cggattaacg tcgcaccatg
    45661 ctcattttta ccggttatcg aatgtaaaaa gccccgcaac gtgttgcggg gctttcatcc
    45721 gttaccggga cgcgaaaaac ttattcagct tcgatgctga taaatttacg gtttttcggg
    45781 cctttaactt cgaatttcac tttaccgtct gctttagcaa acagagtgtg gtcacgaccg
    45841 caacctacgt tagcgccagc gtggaatttg gtaccacgtt gacgaacgat gatgctaccc
    45901 gccagaacgg attcgccacc gaaacgctta acgcccaggc gtttagcttc tgaatcgcga
    45961 ccgttacgtg tggagccgcc agccttttta tgtgccattt gaaatctctc ctcaggtctt
    46021 aggcgctgat gccagtaatt ttcacatcag tgaaccactg acgatggccc tgctgcttac
    46081 gatagtgttt acgacgacga aacttaacga ttttaacttt ctcgccacga ccgtgagcaa
    46141 caacttcagc tttgattacg ccgccatcaa cgaaaggaac gccgattttg acttcttcac
    46201 cgtttgcgat catcagcact tcagcgaact caacagtttc gccagttgcg atgtccagct
    46261 tttccaggcg aacggtctga ccttcgctta ctcggtgttg tttaccacca ctttggaaaa
    46321 ccgcgtacat ataaaactcc gcttccgcgc acaccttttc aatgattcag agtgcgctat
    46381 aaatattcac aatagggcgc gaatattacg caaaacgcac gcctttgaca agtgctacag
    46441 tcaatacacg aagaaaaaaa acacaacttg tacggtaacg tttatctgtg ccattttttc
    46501 agtacaatca ccctatattc ctaaccataa accctaagtt gcctttgttc acagtaaggt
    46561 aatcggggcg aaaagcccgg cttttgcgat gaatttagaa aaaatcaatg agttaaccgc
    46621 gcaagatatg gcgggtgtta atgcggcaat ccttgagcag cttaattccg acgtccaact
    46681 gatcaatcag ttaggctatt acatcgtcag cggcggcggt aaacgtattc gtccgatgat
    46741 tgctgtactg gctgcacgag ctgttggcta tgagggaaat gcgcatgtca ccattgctgc
    46801 cttgatcgag tttatccaca cggcgactct gctacacgac gacgttgtgg atgaatcaga
    46861 tatgcgcagg ggtaaagcta ccgccaacgc tgcatttggc aatgccgcca gcgtgctggt
    46921 aggcgatttt atttataccc gcgcgttcca gatgatgacc agcctcggtt cgctcaaagt
    46981 gctggaagtc atgtcagaag ccgtaaacgt catcgcagaa ggtgaagttc tgcaactgat
    47041 gaacgttaac gatccggaca tcactgaaga aaactacatg cgcgttatct atagcaaaac
    47101 cgcgcgtctg tttgaggctg ccgcgcagtg ttccgggatt ctggctggct gtacgccgga
    47161 ggaggagaaa ggcctgcagg attatgggcg ctatctcggc actgctttcc agttgatcga
    47221 cgatttactc gattacaatg ccgatggcga acagttaggt aaaaacgtcg gcgacgatct
    47281 gaacgaaggt aaaccgacgc tgccgctgct gcatgcgatg catcatggca caccagaaca
    47341 ggcacagatg atccgtaccg ccatcgaaca gggtaacggt cgccatcttc tggaaccggt
    47401 tctggaagca atgaacgctt gtggatctct tgaatggacg cgtcagcgcg ccgaggaaga
    47461 agcagacaaa gccatcgcag cgttacaggt gctcccggac accccttggc gagaagcact
    47521 catcggcctc gcgcacatcg ctgttcaacg cgatcgttaa tcccctcccc tcatcccgcg
    47581 cagcgcgcgg ggtggttcca gtaaattcca taaaaacact tactcagctc tataacacct
    47641 gaaaaacagg ctgtgcatct ttatatattc tgaatattca cactccttac atgaactttt
    47701 tagagcaatc ggccatcagg agtatagtga tgctcgacag aagaagtgtt ctgaatgaaa
    47761 gcgactaact taaggagtga ggaaagatgg aaagtaattt cattgactgg catcccgctg
    47821 acatcattgc gggtttgcgc aagaagggaa cgtcaatggc ggcggagtct cgcagaaatg
    47881 gtttgagttc ctcaacgctg gcgaatgcat tatcgcgccc atggccgaaa ggagagatga
    47941 ttattgcgaa agccctggga actgacccct gggttatctg gccatcacgc taccatgatc
    48001 cgcagaccca tgagtttatc gacagaacgc agttgatgcg tagctacact aaaccgaaaa
    48061 aatgagtggt ctggcggtag ccccgcgaac ggggctgcca gctctcagac gattattcgc
    48121 ctttcacacg ctcaatattt gcacctaaag cgcgcagttt gtcttcaatg cgttcgtagc
    48181 cacgatcgat gtgataaata cgatcaacca ccgtcgtccc ttccgcaata cagccagcca
    48241 gcaccaggct tgctgatgca cgcagatcgg ttgccataac ctgtgcgcca gaaagttttt
    48301 caacaccgcg acaaataacg gtattgcttt cgatttcggc atgcgcgccc atacggctaa
    48361 gctccggcac atgcataaag cggttttcaa agaccgtttc ggtgatgaat ccggtccctt
    48421 ctgccaccag gttcaacagc gtgaactggg cctgcatatc ggtcgggaat gccggatgcg
    48481 gcgcggtacg tacgttaaca gccttcggac gtttgccatg catatccagg ctaatccagt
    48541 cttcgccgac ttcgatgtcc gctccagcat cacgcagttt cgccagcacg gcgtccagag
    48601 tatctggctg cgcgttacgg cagataattt tgccgcgaga aatcgccgcc gccaccagga
    48661 aagtaccggt ttcgatacga tccggtagca cgcgatagac accgccgcct aaacgttcca
    48721 caccttcgat gacgatacga tcggtgccct gaccgctaat tttcgcaccc agcgtaatca
    48781 ggaagttcgc ggtatcgacg atttccggtt cacgcgctgc gttttcaata atcgtggtac
    48841 cttccgccag ggttgcagca cacatgatgg tcaccgttgc gccaacgctg actttatcca
    48901 tcacgatatg tgcgcctttc agacgaccat cgacggaagc tttaacgtaa ccttcttcca
    48961 gtttgatggt cgcgcctaat tgttcgaggc cagaaatgtg tagatcaacc ggacgcgcac
    49021 cgatcgtaca accgccaggc agtgaaactt gcccctgacc aaagcgcgct accagcggcc
    49081 ccagtgccca gatagaagca cgcatggttt taaccagatc gtaaggtgcg cagaatacat
    49141 taacgtcgcg ggcatcaata tgcacagaac cattacgttc tactttcgca cccagctggc
    49201 ttagcagctt cattgatgta tcgacgtctt tcagtttcgg gacgttctgg atctctaccg
    49261 gttcttccgc cagtagagcg gcaaaaagga taggcagagc agcattttta gcgccggaaa
    49321 ttgtgacttc gccctggagc ttcgttggcc cctgaacacg aaatttatcc atttagtttg
    49381 ttctcagtta acaattcata tccgctaccg gcgaatcgcc catagctcaa aagccgttca
    49441 gtttgcgatc gcgcgcccac tccgcagggg tatacgcttt gatcgacaca gcatgaatgc
    49501 ggttatccgc aatatattcc atcagcgggc catagaccgt ctgctgtttt ttaacccgac
    49561 tcatgccgtc aaacaactca cccacggcaa taacctgaaa gtggctgcca tcgccggaaa
    49621 cgtggacttc ctggagggag agagcgttca tcaacacgct ctgaatttca ttattttcca
    49681 tgggatcttc aatcatcagt taataaacca gcgaaacatc ttagagcaaa gttgcgctgg
    49741 cataaataag caaaaagcct cgctgataaa tcagacaagg ctcgacttgc aggcaggttt
    49801 gccggacagg cggttaacgc catatccggc ctgaaaaaat ttaacgaggc agaacatcag
    49861 caggcaaatt atacaatttc gccagggtat acactttgtc gtttaccccc tgaagcgtca
    49921 cattgttgcc ctgctttttc gccagatcga taagatggag cagcagtgcc agtccccccg
    49981 tatccacgcg ggagacacgg ctaagatcga tgcaggtaat ccccttcacc gcttcctcac
    50041 gcatttccca aagcggtagc aaaacgtcct gatccagctc tccggataac gccagcgtgt
    50101 cacccgtctg catccagctc agtgactcgc tcattatttt ttctcttcca gagtgatttt
    50161 ctgttgagaa atcgatttca gttgcgctgt caggccgtcg atacctttgg tacgcagcag
    50221 cgttccccac tcgttttgtt tggtggtgat catactgacg ccttcagcaa tcatgtcgta
    50281 agcctgccag ttgcccgtct gggagttttt acgccactgg aagtccagac gcaccggcgg
    50341 acggccattc gggtcaataa tggtaacgcg aataggcaca atggttttat cgcccagcgg
    50401 ctgttctggc gcaatctgat aggtttgacc gtgatacatc gccagcgcct gaccgtaagc
    50461 ctgcttcagg tactcacgga aagcggcaaa gtaggcttca cgttgagcag gggtcgcact
    50521 cttgtaatac tggcccagca ccagcgcacc ggcgtatttc acctgtacgt atggcagcag
    50581 ttcctgatca acaatggtac gcagataatc cgggttggcc cgaatttgcg gttgctcatt
    50641 cttcaggcga tcgaacgttt tctgcgccgc ctcgtccatc agcttatacg gattggtctg
    50701 gtctgccgcg gttgccgcac tcagaggtgc aatcaccagc aaagcgacca tcattaaacg
    50761 tttaaacatg cgtcggttct cctgaaatta tttcgttgta cccacaggtt cagtggtttc
    50821 attattacct ggcgcagcag ctggcgcatc gccactattc ttattgtcat cgcctttact
    50881 accgtaaagg aactgaccaa tgagatcttc cagcaccatc gcagacttag tgtcctgaat
    50941 tgtatcgcca tccttcagga tagcagtccc cagttccggg tcttcaaaac cgacgtttaa
    51001 tgccagatat tgttccccca gcaggccgga agtacgaatg ctcagcgaac tggtatctgg
    51061 aatgtggtta taacgttgtt caatttccag cgttacgcgc ggcagatagg ttttcgggtc
    51121 cagcgtaata tccgccaccc gacccacaac aacgccacca atactgaccg gagagcgcgc
    51181 tttcaggccg ccaatgttat cgaacgtcgc ataaagcgtg taggtcggtt cagtacgtat
    51241 ggacgtcacg ttcgccgcct tcaggcaaac aaacagcgcc gccagcagtg ctgctaataa
    51301 aaagataccc acccagattt catttttttt cgtttgcatg aactcaattc ccaaacatca
    51361 atgcggtcag cacaaaatcc agccccagaa cagccagaga cgagtggaca acggtgcgag
    51421 tggttgcccg gctaatcccg gcagacgtcg ggatggcgtc gtagccgtta aacaacgaaa
    51481 tccacgtcac cgtgatggcg aacaccacgc tcttaatcag acagttgacc agatccatac
    51541 gccagtcgac ggcattttgc attgccgacc agaagaaccc gctatcaatg cctttccaac
    51601 tgacgccgac cagagatccg ccccagatcc ccacggcgac gaaaataacc gtcaacagtg
    51661 gtaatgaaat aaccccagcc cagaaacggg gagaaataac ccgacgcagc ggatccaccg
    51721 ccatcatctc catactggag agttgctctg tagcgcgcat caggccgatt tctgcggtta
    51781 gcgccgaacc agcacgcccg gcaaacaaca acgcggcaac caccggcccc agttcacgca
    51841 gtagcgataa cgccaccagc atacccagac tggtttccgc actataagtg gtcagaacca
    51901 gataaccttg cagccccaac accattccga tgaacacgcc agaaaccaca ataatcagca
    51961 tcgacaggac gccgacatta tagagctggc gcaccagcag cggcgcatgt ttgcgaaatt
    52021 ccggtttgcc gaccagcgca ttgaataaca ttaacccggc ccgcccgaac gttctcaggg
    52081 ttttaatccc tttatgtccg agcgacgcca gcgcatttaa cagcatgagt ggcttaactc
    52141 cctggtaaaa gatcagcgtg ataatcgccg gcaggatagc ggaacggaac aggcccgtca
    52201 gctatcccgt ccagaaactg acgtacgcgc ggatcaggat tcgcctgcaa cgcctgggca
    52261 ctgccatgag cgacaatttt tttgtccgcc aggatccagg cgtgatccgc aatacttaac
    52321 acttccggca catcgtgaga aaccaccaca caagtcacgc ccagcgcgct gttcagctca
    52381 gaaatcagct tcaccagtac gcccatggtg atgggatctt gcccaacaaa aggttcatca
    52441 aacatgatga gatccggctc cagcgcaatc gcacgcgcca gcgctgcacg ccgcgccatc
    52501 ccaccggaaa gttcagaagg cattagttta gccgctccac gcagccccac ggcctccagc
    52561 ttcatcatca ccgtactatg caacaatggc gcgggaagtt gggtatgttc gcgcagtgga
    52621 taggcgacgt tgtcaaatac gttcatatca gtgaacaacg ccccagactg aaataacatg
    52681 ctcatccgtt tgcgcactgt atacaggcgc gaacgagaca tcgccggaat attctcacca
    52741 tcgaaaagga tctcaccatg atctggtgcg atttgcccgc caatcagacg gagtagcgtc
    52801 gttttaccga tgcccgatgg ccccatgatc gccgtgatct tccctcgcgg cacggtcagg
    52861 gaaatattat cgaagatgca gcgattgcca cgcgtaaaac tgacatcgcg catatcgact
    52921 aaattcgcca cagactgctc cataattcac ccttcgtctt gcgttgattt tctaagcatg
    52981 gcgctcaatt taaccttgaa cccaacatat ttacagaata ttacccgccg tggttagcga
    53041 aagctggcat ttgttttact ttttagccgc ataaagtcaa aattaagaat ccgttacggc
    53101 tttctgaaaa tcttcagcgg accggcgagt atacctgaag aaaggacgtt agatgctttt
    53161 agctacggca ctgttaattg ttggtttact tttggtcgtt tacagtgccg accgcctggt
    53221 ttttgccgcg tctattcttt gccgaacctt tggcatcccg ccgctgatca tcggcatgac
    53281 ggtggtcagt attggtacat cgttaccaga aatcatcgtc tcgcttgccg cgtctctgca
    53341 cgaacaacgc gatttagccg ttggtacagc cctcggctca aacattatca atatattgct
    53401 gatcctcggt ctggctgcgc tggttcgtcc ttttaccgtc cattctgatg ttctacgccg
    53461 tgaattaccc ttaatgttgt tggtcagcgt ggtggccggt tccgtactct atgacggaca
    53521 acttagtcga agcgatggta tctttctcct ctttctggct gtgctatggc tgctgttcat
    53581 tgttaaactt gcacgtcagg ctgaacgtca ggggactgac agcctgacca gagagcagct
    53641 tgcagagctg ccgcgtgacg gcggattgcc cgtcgcgttt ttatggctcg gcattgcgct
    53701 tatcatcatg ccagtggcca cgcggatggt ggttgataac gccacggtgc tggcgaatta
    53761 ctttgccatc agcgagttga cgatgggtct gacggcaatt gctatcggaa ccagcctgcc
    53821 ggaactggca accgcaatag cgggggttcg caaaggtgaa aacgacattg ctgtcggaaa
    53881 tatcattggc gcaaacattt ttaatattgt catcgtgttg ggtttacccg cgctgataac
    53941 gccaggagag attgatccac tggcgtacag tcgtgactac agcgtgatgt tgctggtgag
    54001 cattattttt gcgttgctgt gctggcggcg ctccccgcaa ccgggccgtg gtgtaggggt
    54061 attattaact ggcggattta tcgtatggct ggcgatgttg tactggttat cgccaatact
    54121 cgttgaataa ctggaaacgc attatgtcgc acgtagagtt acaaccgggt tttgactttc
    54181 agcaagcagg taaagaagtc ctggcgattg aacgtgaatg cctggcggag cttgatcaat
    54241 acatcaatca gaatttcacg cttgcttgtg aaaagatgtt ctggtgtaaa gggaaagttg
    54301 tcgtcatggg gatggggaaa tcggggcaca tcgggcgcaa aatggcggca acgtttgcca
    54361 gcaccggtac accttcattt ttcgtccatc ctggtgaagc cgcgcatggt gatttaggca
    54421 tggttacccc acaggatgtg gtgattgcta tctctaactc aggtgaatcc agcgaaatca
    54481 cggccttaat tccagtgctt aagcgtcttc acgtaccgtt aatctgcatc accggtcgcc
    54541 cggagagcag catggcgcgc gccgcagatg tgcatctgtg tgttaaagta gcgaaagaag
    54601 cctgtccgtt agggctggca ccgaccagca gcaccaccgc cacgctggtt atgggcgatg
    54661 ccctcgctgt cgcgctgtta aaagcacgcg gctttactgc tgaagatttt gcgctctcac
    54721 acccaggcgg cgcactgggt cgtaaacttc tgctgcgcgt aaacgatatt atgcatacgg
    54781 gcgatgagat cccgcatgtt aagaaaacgg ccagtctgcg tgacgcattg ctggaagtta
    54841 cccgcaaaaa tcttggtatg actgtcattt gcgatgacaa tatgatgatt gaaggcatct
    54901 ttaccgacgg tgatttacgc cgtgtcttcg atatgggcgt ggatgttcgt cagttaagta
    54961 ttgccgatgt gatgacgccg gggggaatac gtgtgcgccc tggcattctg gccgttgagg
    55021 cactgaactt aatgcagtcc cgccatatca cctccgtgat ggttgccgat ggcgaccatt
    55081 tactcggtgt gttacatatg catgatttac tgcgtgcagg cgtagtgtaa agattcaagg
    55141 ataaacaaca atgagcaaag caggtgcgtc gcttgcgacc tgttacggcc ctgtcagcgc
    55201 cgacgttatg gcaaaagcag agaacattcg tctgctgatc ctcgacgttg atggcgtact
    55261 gtcagatggc ctgatttata tgggcaataa cggcgaagag ctgaaagcgt tcaatgttcg
    55321 tgacggttat ggcattcgtt gtgcgctcac ctctgatatt gaagtcgcta tcattaccgg
    55381 gcgaaaggct aaactggtag aagatcgttg tgccacattg gggatcactc acttgtatca
    55441 ggggcagtca aacaaactga tcgcctttag cgatttgctg gaaaaactgg cgattgcccc
    55501 ggaaaatgtg gcttatgtcg gcgatgatct catcgactgg ccggtaatgg aaaaagtggg
    55561 tttaagcgtc gccgtggccg atgcgcatcc actgttgatc ccgcgcgccg attacgtgac
    55621 gcgcattgct ggcggtcgtg gcgcagtgcg cgaagtttgc gacttattac tcctggcgca
    55681 gggcaaactg gatgaagcca aagggcaatc gatatgagta aagccagacg ttgggttatc
    55741 attgtgctat cactggcggt tctggtgatg atcggcatta atatggccga aaaagacgat
    55801 accgcccagg tggtcgtcaa caacaatgat cccacctata aaagcgagca tacggacacg
    55861 ctcgtctata acccagaagg ggcactaagc tatcgattga ttgctcaaca cgttgaatat
    55921 tattccgatc aggccgtttc gtggtttacg cagccggtac ttaccacgtt tgataaggat
    55981 aaaatcccga catggtccgt aaaagcagat aaagccaagc tgaccaatga ccggatgctc
    56041 tatttatatg gacacgttga agtcaacgca cttgtgccag actctcaact tcgcagaatc
    56101 acgacggata acgcgcagat caatctggtg actcaggatg ttacctctga agacctcgtc
    56161 acgttatacg gaacaacatt taactccagc ggtctgaaaa tgcgcggcaa cttacgcagc
    56221 aagaacgccg agctgattga aaaggttaga acatcctatg aaattcaaaa caaacaaact
    56281 cagccttaat cttgtgcttg ccagctcact tctggccgcc agcattccgg catttgccgt
    56341 aaccggagac actgatcagc cgatccacat tgaatcggac cagcaatctc ttgatatgca
    56401 aggcaacgtg gttaccttta ccggtaatgt catcgtcacc cagggcacca tcaaaattaa
    56461 tgccgacaaa gtggtcgtta cccgtccggg cggcgaacaa ggtaaagaag tgattgacgg
    56521 ctacggtaaa ccggcaacgt tctaccagat gcaggacaac ggtaaacccg ttgaaggtca
    56581 cgcttcccag atgcactacg aactggcaaa agattttgtc gttctgacgg gtaatgctta
    56641 tctgcagcag gtcgatagca acattaaggg cgataagatc acttacctgg taaaagagca
    56701 gaaaatgcag gctttcagcg acaaaggcaa gcgcgtaacc accgttctgg tgccgtcgca
    56761 gctgcaggat aaaaacaaca aaggccagac cccggcacag aagaagggta attaattcgt
    56821 tatggcaaca ttaactgcaa agaaccttgc aaaagcctat aaaggccgtc gcgtggtaga
    56881 agacgtcagc ctgaccgtca actccgggga aattgtcggt ctgctggggc caaacggtgc
    56941 cggtaagacc accactttct atatggttgt aggcattgtg ccgcgcgatg cgggcaacat
    57001 cattattgat gatgacgata tcagtctgct gcctctgcat gcacgcgcgc gccgcggtat
    57061 cggctatctg ccacaggaag cctccatttt ccgtcgcctc agcgtttacg ataacctgat
    57121 ggcggtactg caaattcgtg acgacttgtc tgctgaacaa cgtgaagacc gcgcgaacga
    57181 gctgatggaa gagtttcaca ttgagcacct gcgtgacagc atggggcagt cactctccgg
    57241 gggtgaacgt cgccgtgtag aaattgcccg cgcactggct gcgaatccga aatttattct
    57301 gctcgacgaa ccgtttgccg gtgttgaccc gatctcggtt atcgacatta aacgcatcat
    57361 tgagcacctg cgcgacagcg gcctgggcgt gctgatcact gaccacaacg tgcgtgaaac
    57421 actggcggtt tgtgaacgcg cttatatcgt cagtcagggg catttgatcg cccacggcac
    57481 gcctacagaa atcttacaag acgaacacgt taagcgtgta taccttgggg aagacttcag
    57541 actctgatag ggtagaagtt tgcgacgttt tagcaggaga gtacgattct gaacatgaag
    57601 caaggtttgc aactcaggct tagccaacaa ctggcgatga cgccacagct ccaacaggca
    57661 attcgtctgt tgcagttgtc gacgctggaa cttcagcagg agctacagca ggcgctggag
    57721 agtaatccgc tgcttgagca aatcgacact catgaagaaa tcgacacccg cgaaacgcaa
    57781 gacagtgaaa cgctggacac cgccgacgcg ctcgaacaaa aagagatgcc ggaagagctg
    57841 ccgctcgatg ccagttggga caccatttac accgctggta caccatccgg caccagcggt
    57901 gactacattg acgatgagct gccggtctat cagggcgaaa cgacgcagac cttgcaggat
    57961 tacctgatgt ggcaggtcga gctgacaccg ttttccgaca ctgaccgcgc tattgctacc
    58021 tctatcgtcg atgccgttga tgacaccggt tatctgactg tcccgctgga agatattctc
    58081 gaaagtatgg gcgatgaaga gatcgacatc gacgaggttg aagccgtcct taagcggatc
    58141 caacggtttg atccggtcgg tgtagcggca aaagatctgc gtgactgcct gctaatccaa
    58201 ctctcccaat tcgataaaac cacgccgtgg ctggaagagg ccagattgat cattagcgat
    58261 catctcgatc tgttagccaa tcacgacttc cgcactttaa tgcgcgtcac gcgtctgaaa
    58321 gaagatgtgc tgaaagaagc cgtcaatctg atccagtcgc tcgatccgcg ccccgggcag
    58381 tcgatccaga ctggcgaacc tgagtatgtc attccagatg tgctggtgcg taagcataac
    58441 ggtcactgga cagtagaact caacagtgac agcattccgc gtctgcaaat caaccagcac
    58501 tacgcctcga tgtgcaataa cgctcgcaat gatggtgaca gccagtttat ccgcagcaat
    58561 ctgcaggatg ccaaatggtt gatcaagagt ctggaaagcc gtaacgatac gctactgcgc
    58621 gtgagtcgct gtatcgttga acagcagcaa gccttctttg agcaaggcga agaatatatg
    58681 aaaccgatgg tactggccga tatcgcccag gctgtcgaaa tgcatgaatc gacgatatct
    58741 cgcgtgacca cgcaaaaata cctgcatagt ccacgaggca tttttgaact gaagtatttc
    58801 ttttccagtc acgtcaatac cgagggcggc ggcgaagctt cctccacggc gattcgtgcg
    58861 ctggtgaaga aattaatcgc ggcggaaaac ccagcgaaac cgttgagcga cagcaagtta
    58921 acctctttgc tgtcggaaca aggtatcatg gtggcacgcc gcactgttgc gaagtaccga
    58981 gagtctttat ccattccgcc gtcaaaccag cgtaaacagc tcgtttgacc caaccgataa
    59041 ggaagacact atgcagctca acattaccgg aaataacgtc gagatcaccg aggcactgcg
    59101 cgaatttgtt acagccaaat ttgccaaact tgagcaatat tttgaccgaa tcaaccaggt
    59161 ctatgttgtt ctgaaagtgg agaaagtcac ccacacctca gatgcaacac tgcatgtaaa
    59221 cggtggtgaa attcatgcca gcgcggaagg tcaggacatg tacgctgcca ttgatggctt
    59281 aattgataag ctggcacgcc agttgaccaa acataaagat aaactgaaac aacactaatt
    59341 gtccgggcaa ttagcatgtg catggcggcc tgttgtgcgg cacaacgggc catttgtacg
    59401 gttaatgctc cgagcctgtt ccactgtttg agtgggcagg ttcttaggtg aaattatgac
    59461 aaataatgat acaactctac agcttagcag tgttcttaac agggaatgta cgcgaagccg
    59521 cgtccactgt cagagcaaaa aacgcgccct ggaaatcatc agcgagctgg cggcgaaaca
    59581 acttagcctg ccgcctcagg tggtttttga agctatcctg acgcgggaaa aaatgggcag
    59641 taccggtatc ggcaatggta ttgccattcc gcatggcaaa ctggaagaag atactctgcg
    59701 cgccgttggc gttttcgttc agctcgaaac gcctatagct ttcgatgcca tcgacaacca
    59761 accggtggac ttactttttg ccctgctggt gccggcagac caaactaaaa cgcacctgca
    59821 tactctgtcg ctggtggcga aacgtctggc ggacaaaacc atctgccgcc gtttacgcgc
    59881 agcccagagc gatgaagagc tgtatcaaat cattacggat accgaaggta ctccggatga
    59941 agcgtagtta ttcggtaatg tctcttttag acgttgtgag gagaaacagt acatggtact
    60001 gatgatcgtc agcggacgtt caggttcagg taaatctgtc gccctgcgtg cgctggaaga
    60061 tatgggtttt tactgcgtgg ataaccttcc cgtagtgttg ttacccgatc tggctcgaac
    60121 tctggccgat cgcgagattt ctgccgccgt cagcattgat gttcgtaata tgccggagtc
    60181 accagaaata ttcgaacagg cgatgagtaa cctgcctgac gctttctcac cgcaactact
    60241 gttcctggat gccgaccgta ataccttaat tcgtcgttac agtgacacgc gccgactgca
    60301 tccgctttcc agcaaaaacc tgtcgctgga aagtgctatc gacaaagaaa gcgatttgct
    60361 ggagcctctg cgttcgcgag cggatctgat tgtcgatacc tcagaaatgt ccgttcacga
    60421 gctggctgaa atgctgcgta cccgtctgct gggtaaacgt gaacgcgaac tgaccatggt
    60481 ctttgagtct ttcggcttca aacacggtat ccctatcgat gcagattacg tctttgacgt
    60541 gcgcttcttg ccgaacccgc actgggatcc gaaactgcgt ccaatgacag gtcttgataa
    60601 acctgtcgcc gcgttcctcg accgccacac agaagtacac aattttatct accagacgcg
    60661 aagctatctt gaactatggt tacctatgct ggaaaccaac aaccgtagct acttgacggt
    60721 cgccattggt tgtaccggcg gaaagcaccg ttcggtgtat attgcagagc aactggcaga
    60781 ctacttccgc tcgcgcggta aaaacgtcca gtcacgccat cgtacgctgg aaaaacgtaa
    60841 accatgaccg tcaagcaaac tgttgaaatc acaaacaagc tgggcatgca tgcccggcct
    60901 gcaatgaagc tgtttgaatt aatgcagggt tttgacgctg aagtgctgtt acgtaatgac
    60961 gaaggcaccg aggccgaagc caacagcgtt attgcgctgc tgatgttgga ttctgccaaa
    61021 ggacggcaga ttgaagttga agcgaccggc ccacaggaag aggaagcact ggccgccgtt
    61081 atcgccctct ttaactctgg ttttgatgaa gattaatctt catcactttg acatacaact
    61141 cccttcaaac tccccccgac aataagaaaa tcacgtactg aaatcgttct caatcaacgt
    61201 catttgtaca ttttgtgcgc ttttcacttt cagaagaacc ttaagaaaac cttaagaggc
    61261 attgtttagg ttttgtttaa gttaatcgac catactggag atcgtcagaa aatatttcca
    61321 ggagatggca tgattcgctt atctgaacaa agtcccctgg gcaccggaag acatcggaaa
    61381 tgttatgcgc atccggaaga tgcccaacgc tgtatcaaga ttgtctacca tcgtggcgat
    61441 ggtggcgata aagagatccg ccgtgagtta aagtactacg cgcatcttgg tcgccggtta
    61501 aaagactgga gtggaatacc gcgctatcac ggtaccgttg agacggattg cggaaccggt
    61561 tatgtctacg atgtgattgc agattttgac ggcaaaccct ccattacgct gaccgaattt
    61621 gctgaacaat gccgttacga agaagacatt gcacaactgc gccagctact gaaacagctg
    61681 aagcgttatt tgcaggataa ccgtatcgtg acgatgtcgc tgaagccgca gaatatcctc
    61741 tgtcaccgca tcagtgagtc agaggttatc ccggtcgtct gtgacaacat tggtgaaagc
    61801 acattgattc cactggcgac ctggtcgaag tggtgttgtc tgcgtaagca agagcggtta
    61861 tggaaacgat ttatcgccca accggcgctg gctattgccc tgcagaaaga tctgcaacct
    61921 cgtgagagca aaacgctggc cctcacttcg cgcgaagctt aatccagctg gtgttgttgc
    61981 ataaacggct caccgcctaa ctgatacatc tgccgtaaaa tccacgcctg acggctacgc
    62041 acgtagcccg atggcgagga gactttgaaa cgaagtggat taggtaatac agctgccagt
    62101 aatgcagctt ccgaccgggt aagtttgctc gcgggtttgt ggaaataacg ttgtgccgca
    62161 gcttcgacgc caaacacacc gtcgccaaat tcggcgatat tcaggtaaac cgtcaggata
    62221 cgctttttgc tccagactgt ttctatcccc agcgttaatc cagcttccag cccttttcgt
    62281 acccagctac gcccatccca taaaaagaga tttttggccg tctgctgaga aatcgttgaa
    62341 gcaccgcgaa tacggttttc attgcgctcg ttgtgcgcca gggctttctc aatggaagcg
    62401 acatcaaagc cccagtgctc aggaaatttc tgatcttctg cggcaatcac cgccagtccc
    62461 atccacggcg agatttgatc catactgacc cagtcagaat gtgccacgta acgaaaattg
    62521 ccatgcagcc aggcgctgac ctgtcgctcg accattaccg ctgagaaggg aacaggcgca
    62581 acactaaaca acgcgatgcc cccgccccag aaaacggcga ggacaaccat taaccgcaaa
    62641 agaaaacggc gaacgaaact aaacaccgtt aagcggcttt tactcattca gccagaacca
    62701 gcacgcggga aaccagctta tcaatgccgc tcgccgcttc tgcaatgttc tgcgccagca
    62761 tatatgctgg ggtggtgaca atcttattgt cttcatcaac cacgatatca tcgacaggac
    62821 agggcacatg ctccgcgccc atctcttcca gcacttctgc ggtatcgata tcagtaccga
    62881 tggtcaaacg cagcgggaaa tcgaaaattt tcggcagcat cgccggggca atacacataa
    62941 aaccaagcgg ttttccggcc tgatgcatcg cttgtgccag cgcctttaat tcacggtcaa
    63001 cggtgcattc gctaccaaga gtggcaaaat tgcttaaatt cttcgccgcg ccaaaccccc
    63061 ccggcacaat caacgcatcc agttcagcgg catcggcctg ggccagagga cggatttcac
    63121 cgcgcgttat tcgtgccgct tcaatcagca cgttgcgcgt ttccgtcatc gcttcgccag
    63181 ttaaatggtt gataacatca acctgctgct tatccggtgc aaagcagacc gcctgcgcac
    63241 cgctgcgtga aatagctaac aacgtcaaca ccgcttcatg aatttcagaa ccgtcataga
    63301 cgccgcatcc gctcagaatt acgccaattt tcttcattgt gatcatcctt tcgcaattga
    63361 ctgaaacaca ttaatttttt taataaaaat ggtacgcatc acacatttaa ctgattcatg
    63421 taacaaatca tttaagtttt gctatcttaa ctgcgtgcgg cctgaaaaac agtgctgtgc
    63481 ccttgtaact catcataata atttacggcg cagccaagat ttccctggtg ttggcgcagt
    63541 attcgcgcac cccggtctag ccggggtcat tttttagtgg cttttgccac ccacgctttc
    63601 agcacttcta cgtcgtgacg ccactcttct ttcatctctt caatccattc accgacgtta
    63661 tcttcccagg caggaaggtc cggagactga atttgctgac ccagctgttg cagatggcgt
    63721 aaccccactg accccgccgc acctttaatt ttatgtcctt cctctacaat gcctttttta
    63781 tcctgcgccg tcagattcga ctccagcacg ctgacatagc ccggcatcat cttctcaaac
    63841 accgctaacc cgtcggtgat cagcttcggt cctacaagtt cgagatactg ttccagcatg
    63901 ggaatatcga gcaatgcttc tgatttactg ttctcttctg tcgtcaccgt actctcctca
    63961 tcatcctggg tatcccagaa tttcttgatc atcgcggtta gcgccggaac cgaaagcggc
    64021 ttactcagca catcatccat tccagcattg aggtactctt gtttgtcttt cagcacgtta
    64081 gcggttaagg ccaccagcgg cggtaaatcc tcgcgcggat aacgcttcgt cagttcacga
    64141 gagatatcca gcccggtcat atctggcaac tgaatatcca gcaacaccag gtcgtattcg
    64201 cccggtttaa acatctccag cgccgccttg ccggtcatgg cgacatcaac gctgttacct
    64261 aatttttcca gcacagaacg cgcaacaatc acgttcagtt caatgtcttc caccagcagc
    64321 acattcagcg ccggtaaagg catatcgtct tcatcaaacg catcatcgac ctcttctgct
    64381 accgacggtg cgtggatcgt caacgtaaag gttgaacctt tgccctgttc gctggtaacc
    64441 gtaatatcgc cgcccatatt tttcgccaga cgacgagaaa cggccagacc aataccggtg
    64501 ccggtggcag gtttaccgcc atgactgtct ttcacctggt aatacatggc gaaaatttta
    64561 tccagctcat cctgcggaat gccgatacca gagtcttcca cttcaaaatg cagcatatcg
    64621 ccttcatcgt agcgcacgcg cacggtaacc tggccttgct gggtgaattt gacggcgtta
    64681 ctgatgaggt tccacaggat ctgccgtaaa cgcgtcccgt cggtaatgac ctgatgcggt
    64741 aatggcagcg tcggctccag gttaaagcgc aatccttttt gttgcgcctg caatgcggag
    64801 agattttcca gatcggcaag gaagctggtg aaatcaaccg gttgattatc aagctggacc
    64861 ttgcgccgtt ccatcttatc catgtcgata atatcgttaa agatattccc cagcgtgacg
    64921 gccgaaacat ggatggtctt gagatatttt tcctgctcgg cggtgagttc ggtatccagc
    64981 agaatacggc tcaggccgac gataccgttc agcggtgtac gcaattcgtg actgatggtg
    65041 gagataaacg tcgttttgtc gcggctggcc cgttcaagcg catcctgata ccgcttacgc
    65101 tcggtaatgt cgcgaccaaa gcccatcaaa ccgtgacgtt tacccacgcg gtcgtagtac
    65161 ggcactttac ggatttcaaa gcaggctttg cgcccgtccg ggtaatccag ccactgttca
    65221 taggtcagtg acacattatg acggaacact ttttcatcgg tttcaatgac ttttgcggcg
    65281 gcttccggtg agtaaacatc agcaggtttc aggtgaacca gttgtttttc gctttttccg
    65341 gtcagcagct ccatcgcgcg gttacagccg gaaaactctt tatcttcgtt acgataaaaa
    65401 accaggtcgg gtgaagcatc aaggaaggaa cgtaagaatg aggattgctg ctcgagctga
    65461 atttgtgtct cttcgcgctc tttgatttca attttcagtt ggccgaaggt ttcctgcagt
    65521 tctgcttccg ctttttcacg aacggcgatt tcctgattta gctgggcaat attatctttt
    65581 aactgaacgt tgaggctcaa atcgcgctcg cgcatctcct ccagtttttg caccagccgt
    65641 gacagacgtt gtcgtgactc ctccagttgc tcgacgacca ccgatagaaa gtagaccgcc
    65701 cacggcgtaa tcagcaaacc aaagaagata gaacgaataa catcaatgct ttcgacctga
    65761 ccatgcagca ccatggttac cgccatttgt accacaatgg caagaacgac gagggccagc
    65821 gccagcaaca ttgagaagcg caccagacct aacttcatca tcaggtcaac ataatactgc
    65881 gccagcagac gaatttgctt cattagggaa ttccttcacg acaacctgcc ccaataatac
    65941 ccaattctga cagttacgtt ataaattgtg tgagaaatgc ggaattcccc tcaacgacct
    66001 actccgttgg aggtatccac ggacgttcca gcgccgatcc ctgtacgcca tgttcattca
    66061 gatagcgatc cagctcgacc attcccgtcc atcgattttc gcaccacaga ggagcaagca
    66121 gcgtcgggcg acgggcgctg gcggaaatgc ggtggtagat cacttccggt ggcgtatggc
    66181 gaatcatttc tccggcagtg agcgtgtaat cctccagttc aataccgttc aaacgccccg
    66241 cttcccaggc tttcgccata atgctgcctt tcacaatatg cagcggatgc agctttatgc
    66301 cgtccacgcc ggtttcaacc actcgctcca gcgtttgcaa acattcagcc tgcccttcgc
    66361 caggcaggcc gacaattaag tgggaacaca ccttcagccc gcgctgacgt gccagctggg
    66421 ttgtacgctg ataacaggca aaatcatggc cgcggttgat gcgatgcagt gttttgtcgt
    66481 gggcggtttg tagccccagc tccagccaca cttcgtaacc ctggtcctta tattcgcaaa
    66541 gcagatccag caccgcatcc ggcacgcagt ccgggcgggt accaacacac aaaccgacaa
    66601 tattggcctg gctcaccgcc tgctgataca tagaacgcag cacctgaact tccgcaaagg
    66661 tgctggtata cgcctgaaag taggccagat agcgtttagc gcggttaact aaattcgcct
    66721 ggtgcgccag ttgctcggca atggaacgat gctgctgcgc ttcatcggca aacgaggcaa
    66781 cattacagaa tgtgcagcca ccacgcccga tggtaccgtc acggttaggg cagctaaaac
    66841 cgccgtgtag cgtcagctta tgcacctttt gcccataacg acgggtgaga tcaccaccaa
    66901 acatattgac taatttctgt aactgcataa tctgatagac cgcgccttga aaagaggcca
    66961 aagcctgcca tttttagctc aattcggcga tgacctggat caatcgtccc ggcccgcttt
    67021 tatcaactgc ataatcaatc aaaattaccg aaatttcatg cataatcaca taaatcactt
    67081 ttgcttatct tgtgtcagat ttttttatct ccttatggat tttaggcaaa aacagtagca
    67141 tgaaacgtca ttaccaatta aggcagtata aaatgctggt tttctcgtca gttcaaggca
    67201 ggataagggt taacacgcct ttatgacagt caggaattga ctgtttctct aacgacttcc
    67261 cttttagcct taaagataaa atccatttta atttcagtaa tttaataaag aattttgcgc
    67321 taaagcacat ttctgtacca ataagcttgc catttgacct gtatcagctt tcccgataag
    67381 ttggaaatcc gctggaagct ttctggatga gcagcctgct catcatattt atgcagtaat
    67441 tgagatcccc tcttcaccgt attaaccgat gcgaaaagga caacaagggg gcgaatgcga
    67501 ggcgcgcgta tgacacgcaa accccgtcgc cacgctcttt ctgtgcccgt gcgcagcggt
    67561 tcggaagtgg ggttcccgca gagcctgggg gaggttcacg atatgttgta cgataaatcc
    67621 cttgagaggg ataactgtgg tttcggcctg atcgcccaca tagaaggcga acctagccac
    67681 aaggtagtgc gtactgcaat acacgcactg gcccgcatgc agcaccgtgg cgcgattctc
    67741 gccgatggta aaaccggcga cggttgcggc ttgctgttac aaaaaccgga tcgctttttt
    67801 cgcatcgttg cgcaggagcg cggctggcgt ttagcaaaaa actacgctgt cgggatgctc
    67861 ttcctgaata aagatcctga actcgccgct gccgcacgcc gcatcgttga agaagagctg
    67921 caacgcgaaa ccttgtcgat tgtgggctgg cgtgatgtcc ccactaacga aggcgtgctg
    67981 ggtgaaatcg ccctctcctc tctgccccac attgagcaaa ttttcgttaa cgctccggca
    68041 ggctggcgtc cgcgcgatat ggagcgccgt ctgtttatcg cccgccgccg cattgaaaag
    68101 cgtctcgaag ccgacaaaga cttctacgtc tgtagcctgt cgaatctggt gaacatctat
    68161 aaaggtctgt gtatgccggc ggatctgccg cgcttttatc tggatcttgc ggacctgcgt
    68221 ctggaatcgg ccatctgcct gttccaccag cgcttctcca ctaacaccgt accgcgctgg
    68281 ccgctggcgc agccgttccg ctacctggcg cataacggtg aaatcaacac catcaccggt
    68341 aaccgccaat gggcgcgtgc gcgtacctat aaattccaga cgccgctgat ccctgatctg
    68401 cacgacgccg caccgttcgt caacgaaacc ggttctgact ccagttcgat ggataacatg
    68461 ctggaactgc tgctggcagg cgggatggat atcatccgcg ccatgcgtct attagtacca
    68521 cccgcctggc agaacaaccc ggatatggac ccggaactgc gtgccttctt tgactttaac
    68581 tccatgcata tggagccgtg ggatggcccg gcgggcatcg tgatgtccga tggtcgcttc
    68641 gctgcctgta acctcgaccg taacggtctg cgtccggcac gctacgtcat taccaaagac
    68701 aagctcatca cctgcgcctc tgaagtcggt atctgggatt atcagcctga cgaagtggtc
    68761 gaaaaaggcc gcgtcgggcc aggcgaactg atggtgatcg acacccgcag tgggcgtatt
    68821 ctgcactcgg cagaaaccga tgacgatctg aaaagccgcc atccatataa agagtggatg
    68881 gagaaaaacg tccgccgact ggtaccgttt gaagatctgc ccgatgaaga agtgggtagc
    68941 cgcgaactgg acgacgacac gcttgccagc taccagaaac agtttaacta cagcgcggaa
    69001 gagctggact ccgtaattcg cgtactgggc gaaaacggtc aggaagcggt cggttcgatg
    69061 ggcgatgata ccccattcgc cgtgctctcc agtcagccgc gcattattta cgactacttc
    69121 cgccagcagt ttgcccaggt gactaacccg ccaatcgacc cgctgcgtga agcgcatgtt
    69181 atgtcgctcg ccaccagtat cggtcgtgaa atgaacgtct tttgcgaagc agagggccag
    69241 gcgcaccgtt taagctttaa atcgccgatt ctgctctact ccgatttcaa acagctcacg
    69301 acgatgaaag aggagcacta ccgcgcagat acgctggata tcacctttga cgtcactaaa
    69361 accacgctcg aagcgacagt caaagagctg tgcgacaaag ccgaaaaaat ggtacgtagc
    69421 ggcaccgtgc tgctggtgct ctccgaccgg aatatcgcta aagatcgcct gccggttcca
    69481 gccccgatgg cggttggcgc gatccagacc cgtctggtcg atcaaagcct gcgttgcgat
    69541 gccaacatca tcgtcgaaac cgccagcgcc cgcgatccgc accacttcgc cgtgttgctg
    69601 ggcttcggcg cgacggctat ttatccatac cttgcctatg aaacgctggg ccgcctggta
    69661 gacacccatg cgattgccaa agattatcgt accgtgatgc tcaactaccg taacggcatc
    69721 aacaaaggct tgtacaaaat catgtccaaa atgggcatct ccaccatcgc ctcttaccgc
    69781 tgctcgaaac tgtttgaagc ggtcggtctg cacgatgatg tagtgggcct gtgcttccag
    69841 ggggcggtca gccgcattgg tggggcgagc tttgaagact tccagcagga tctgctgaat
    69901 ctgtcgaaac gtgcctggct ggcgcgcaag cccatcagcc agggcggcct gctgaaatac
    69961 gtccacggcg gcgaatacca cgcctacaac ccggacgtgg tgcgcacgct gcaacaggcg
    70021 gtacagagcg gcgagtatag cgactatcag gaatacgcga agctggttaa tgagcgtccg
    70081 gcaaccacgc tgcgcgatct gctggcaatt acgccgggtg aaaacgcggt caacattgct
    70141 gatgttgaac cggcaagcga actgtttaaa cgctttgata ccgccgcgat gtctatcggc
    70201 gcgttaagcc cggaagccca cgaggcgctg gcggaagcga tgaatagcat cggcggtaac
    70261 tcgaactccg gtgaaggcgg cgaagacccg gcgcgctatg gcactaacaa agtgtcgcgc
    70321 atcaagcagg tggcttctgg tcgcttcggc gttactccgg cgtatctggt caatgccgac
    70381 gtcattcaga ttaaagtcgc ccagggcgcg aagccgggcg aaggcggtca gttgccgggc
    70441 gacaaagtca ctccttacat cgccaaactg cgctattcgg tgcccggagt gacgctgatc
    70501 tccccgccgc cgcaccacga tatctactct atcgaggact tagcgcagct cattttcgac
    70561 ctcaagcagg ttaacccgaa agcgatgatc tccgtgaagc tggtttccga accgggcgta
    70621 ggcaccatcg cgactggcgt ggcaaaagct tatgccgatt taatcaccat cgcaggctat
    70681 gacggtggca ccggcgcaag cccgctgtcg tcagtgaaat acgcaggttg cccgtgggag
    70741 ctggggcttg ttgaaaccca gcaggcgctg gttgccaatg gtctgcgtca taagatccgt
    70801 ttgcaggtcg atggcggcct gaaaaccggt gttgatatca tcaaagcggc gattctcggc
    70861 gcagaaagct tcggcttcgg cactggcccg atggtggcgc tcggctgtaa atatctacgt
    70921 atttgccatc tgaacaactg cgcaacgggt gtagcaactc aggatgacaa actgcgtaag
    70981 aaccactatc acggcctgcc gttcaaggtt accaattact ttgaatttat tgcccgtgaa
    71041 acccgcgagc tgatggcaca gctgggcgta acgcgtctgg tggatctgat tggtcgcacc
    71101 gacctgctga aagaactgga cggtttcacc gccaaacagc aaaaactggc gctgtcgaag
    71161 ctgctggaga ccgccgaacc gcatccaggt aaggcactct actgcaccga aaacaaccca
    71221 ccgtttgata acggcctgct gaacgcgcag ttgctgcaac aggcgaaacc gtttgtcgat
    71281 gaacgccaga gcaaaacctt ctggttcgat attcgcaaca ccgaccgttc tgtcggcgcg
    71341 tcgctttcag gctatatcgc ccagacgcac ggcgatcagg ggctggcagc cgatcctatc
    71401 aaagcgtact tcaacggcac cgcaggccag agtttcggtg tgtggaacgc gggcggcgtg
    71461 gaactgtacc tgaccgggga tgccaacgac tatgtcggta aaggcatggc gggcggctta
    71521 atcgccattc gtcctccggt tggttccgcc ttccgcagcc atgaagcaag tattatcggc
    71581 aacacctgcc tgtatggcgc gaccggaggt cgtctgtatg ccgcaggccg cgcgggtgaa
    71641 cgtttcggcg tgcggaactc cggtgctatc accgtggtag aaggcattgg cgacaacggc
    71701 tgtgaatata tgacgggcgg tattgtctgt attctgggta aaaccggcgt taacttcggt
    71761 gcgggcatga ccggcggctt cgcttacgtt ctcgatgaaa gcggtgattt ccgcaaacgc
    71821 gttaacccgg aactggtcga ggtcttaagc gttgacgatc tggcgatcca tgaagagcat
    71881 ctgcgcggtc ttatcaccga gcatgttcag catactggct ctcagcgcgg tgaagagatt
    71941 ctggcgaact ggtcaacctt cgccactaaa tttgcgctgg ttaaaccgaa gtccagtgat
    72001 gtaaaagcac tgctgggtca tcgtagtcgt agcgcagcag agttgcgcgt gcaggcgcag
    72061 taaggggtag caacaatgag tcagaatgtt tatcaattta tcgacctgca gcgcgttgat
    72121 ccgccaaaga agccgctgaa gatccgcaaa attgagtttg ttgaaattta cgagccgttt
    72181 tccgaaggcc aggccaaagc gcaggctgac cgctgcctgt cgtgcggcaa cccatactgc
    72241 gagtggaaat gtccggtaca caactacatc ccgaactggc tgaagctcgc caacgagggg
    72301 cgtatttttg aagcggcgga actgtcgcac cagaccaaca ccctgccgga agtttgcgga
    72361 cgagtctgcc cgcaagaccg tctgtgcgaa ggttcctgca ctctgaacga tgagtttggc
    72421 gcggtgacca tcggcaacat tgagcgttat atcaatgata aagcgttcga aatgggctgg
    72481 cgtccggata tgtccggcgt gaagcaaacc ggtaaaaaag tggcgattat cggcgcaggc
    72541 ccggcaggtc tggcgtgtgc ggatgtcctg acgcgtaacg gcgtaaaagc cgttgtcttc
    72601 gaccgtcatc cagaaattgg cgggctgctg accttcggta ttccggcctt caagctggaa
    72661 aaagaggtaa tgacgcgtcg ccgtgaaatc ttcaccggca tgggtattga attcaaactc
    72721 aataccgaag tgggccgcga cgtgcagctg gacgatctgc tgagtgatta cgatgccgtc
    72781 ttccttggcg tcgggactta tcagtcaatg cgcggcgggc tggaaaacga agacgccgat
    72841 ggcgtgtacg cagcgctgcc gttccttatc gccaacacca aacagttaat gggctttggt
    72901 gaaacccgcg acgaaccgtt cgtcagcatg gaaggcaaac gcgtggtggt ccttggcggt
    72961 ggcgacactg cgatggactg cgtgcgtacg tccgtgcgcc agggagcgaa gcacgttacc
    73021 tgtgcctatc gtcgtgatga agagaacatg ccgggttccc gccgcgaagt gaaaaacgcg
    73081 cgggaagaag gcgtagagtt caaattcaac gtccagccac tggggattga agtgaacggt
    73141 aacggtaaag tcagcggcgt aaaaatggta cgtaccgaaa tgggcgaacc ggatgccaaa
    73201 ggccgtcgcc gcgcggagat cgttgcaggt tccgaacata tcgttccggc agatgcagtg
    73261 atcatggcgt ttggtttccg tccacacaac atggaatggc tggcaaaaca cagcgtcgag
    73321 ctggattcac aaggccgcat catcgccccg gaaggcagcg acaacgcctt ccagaccagc
    73381 aacccgaaaa tctttgctgg cggcgatatc gtccgtggtt ccgatctggt ggtaaccgct
    73441 attgccgaag gtcgtaaggc ggcagacggt attatgaact ggctggaagt ttaagctctg
    73501 tcccagcgcc gcctcctcag aaaagagtcg gcgctgaaat tctcctttct ttctggaaag
    73561 cgcctcgcat aatctgaaga aaaccaatgt aatacgctca tctggattgt taccggtaga
    73621 tgcccggcgt atagctgatt ccattattca acgcagcgcc atataaaggg acagataatg
    73681 gaatcgctct cagagagaac ctcaacaggc taccagcaaa tccacgacgg tattattcat
    73741 ctggtcgata gcgcccggac ggaaacggta cgtagcgtta acgcgctgat gaccgcgaca
    73801 tactgggaaa ttggccgacg aattgtcgaa tttgaacaag gtggcgaggc cagggctgcg
    73861 tatggtgcgc agctaatcaa gcgactatca aaggatttaa gtctaaggta taagcgtggg
    73921 ttctctgcaa aaaacttacg ccaaatgagg cttttttacc tcttttttca acatgttgaa
    73981 attcgccaga cagtgtctgg cgaattaaca ccattgggaa ttccgcagac accatctgca
    74041 gaattcccat cggcaaagat ttggcagacg ctgtctgcca aatctttccc cctgccctgg
    74101 tccacctacg tccgtttact ttccgttaaa aacgctgacg cccgcagctt ttatgaaaaa
    74161 gagacgctcc gctgtggctg gtctgttcgc cagctagaac ggcaaattgc gacccaattt
    74221 tatgagcgaa cactactgtc acatgacaaa tcagccatgc tgcaacaaca cgctcctacc
    74281 gagacgcata ttcttccgca acaggcgata cgcgatccct ttgtgctcga atttctggaa
    74341 ttgaaagatg aatattcaga atctgatttc gaggaggcgc tgatcaacca cctgatggat
    74401 ttcatgctgg aacttgggga tgattttgcc tttgttggtc ggcagcgaag gttacgcatt
    74461 gatgacaact ggtttcgtgt cgatctgctg tttttccacc gccgtttacg ctgcctacta
    74521 atcgtcgatc taaaagtggg caaattcagc tatagcgatg ccggacagat gaatatgtat
    74581 ctcaactacg ccaaagagca ctggacgcta ccggatgaaa atccgcccat cggtctggtt
    74641 ctctgtgcag agaaaggagc cggagaagca cattatgctc tggcaggttt gcctaacacc
    74701 gttctggcaa gcgaatataa gatgcaacta cctgatgaga aacgactcgc agatgaactc
    74761 gttcgaacac aggcggtgct agaggaaggc tatagacgcc gttaatttca gcggtttcga
    74821 attctgcaga cagtgtctgc agaatcttta aaacgaacta agttttaagc cattaaatca
    74881 gccttaacct tcaccacaac ctttttaatc tctccaggct cgccgacaac gcatcctggt
    74941 ttatgcggtt cacctgccat aaacacggcg aacattcccg gttttaagat gatggcttgc
    75001 tcgttatcaa tggtgctgca aagctgataa tcatcctcat ggtggaactc ttcacactgg
    75061 cgcgcagtgc ctgccatgcc aaacagaatc cgttcctcac cgtttaataa cagttggata
    75121 tcaatgtatt gctcgtgcaa ttccgctttt ttctcgacgg gcgattgagt gttaaacgtc
    75181 atgacattca taaagatatt gtcgccctgt aattcgtaac gacccggcgc tttttcttgc
    75241 ggtctggcag ctaatgccag cgttaacgcg tcctgtaacg caggatgtaa cccagcagac
    75301 ggtaatgact gtacttcacc catcatcata atttttctcc ctgggccaac agcgcagccc
    75361 caagtaaacc tgcatcgtgg cggtagtgcg ccgccagtaa atcaacatga aatgccgctg
    75421 gctcctgcgc cagatacgtt tccaccagcg ccagataccc ttctgccagc ccaacgctgc
    75481 caccgaccac cacgcactgg caatcagttg tggctttaat atcagcgatc agccttgcaa
    75541 gcgtacgtgc ggagcggtga atcagctgct gcgcctgctc gtcgccctgc ccggcgcgtg
    75601 tgaaaatagt tctcgcatcc gcgcccgcca gttcaccctg cgctgccgcc gcaatgccgc
    75661 gaccagaagc aatcgcctcc acgcaacctg tgcgtccaca gccacagacc gggccgtgtg
    75721 gatcggcaag cgtatgcccg atatgccccg ccagaccgcc agggccggta agcagtttgc
    75781 cgccgctcac tacaccgccg ccgacgccag tggaaacggt gataaacacc atctcggtta
    75841 catcgccttc cagcgcctga tactccgccc atgctgcagc ctgcgcgtcg ttaatggcaa
    75901 tggtcggcaa attggtaagt tgttccagcg ttttgactaa cggaaagtgt agcaatccac
    75961 cgagattatg cggattcagc gccagcaaac tgccgtcgcg aataattccg gttgaagcga
    76021 tggcaacccg ctgcgcatga gcttgcaacg gagagactaa tgcggataag gcatcacgca
    76081 aggcttctgg tgtctggctg gctggcgtag gaagttcacg acgatcgcgg atctgcccgt
    76141 cagcgccaat cagcgcggcg gcaagtttag taccgccgat atcaatcgcc agcgtggtca
    76201 tagcaccgcc tttttcatcg ctgtgttgta ccactgacaa atgtgttcaa gacgcgtaat
    76261 ggcagaaccg accgtcaccg cccacgcgcc gtggcgcatc gcatctgccg cctgagcagg
    76321 cgtgttgtaa cgcccttcgg caatcaccct acatccggcg tcgctcaacg ttttcaccag
    76381 cgccagatcc ggctcttctg gcgtttcagg cgtggtatag ccagaaagcg tagtgccaat
    76441 aatttcggct cccagctttt ggcatgccag gccgtcttcc ggcgttgagc agtcggtcat
    76501 cgccagtaaa ccgtgatggt gaatacgtgc cagcagcgtt tcaacaggca ccggacgcgg
    76561 gcggtcggtg ccgtcaatgg cgataatgtc cgcgcccgcc tgcgccagcg catcaacatc
    76621 ttcaatatag gccgtgatgc gtaccggaga atcctccaga tcgcgtttca caattccaat
    76681 aatcggcacg ctcaccaccg cacgcgtggc ttgcagattt gccacacctt caatgcgaat
    76741 ggcaaccgcg cccgcctgtt ctgccgctaa tgccatggcg gcgacgattt cgggtttatc
    76801 gagcgggctg tccggaaccg gctggcagga gacaatcagg ccaccgttag cagcgatttt
    76861 ttgatccagt tgtgcaagta acgacataca tcttccctta gcgaaaggcc cggtacatag
    76921 accgggcaac aggattaact tttggttttg actaaatcgt ttttggcgct gccaaatggc
    76981 acggcaccgc taaatggttt gccgtcgata gcgtcatgag tacgcaatgc ttccgggcgc
    77041 agccaacgct gaacgcggga aggcatatcc agaccaatca gcaggatcac cacaaatgtc
    77101 agactgaacg atagcgatgc cagcgcagta cccagatcca gacgttgagc gatcaacgcg
    77161 ccgatgattg gggccagtgc gccgcccaat gcgccaacgt tgtaggtaaa gcccaggccc
    77221 gctgcacgct ggtcggtatc gaaataaccg ccaatcagtt tcggtaagat cccggcgatc
    77281 ccttgtccaa gcatttgctg gaagaacagt aacagaccga gcacccagac gtttgcgccg
    77341 ccaatcgcaa ataccggaat aatcaacagc tgcgaggcca acaggctaca aacgtaggct
    77401 ttgcgggttc ccagccagtc accgaggaag ccgccgacac agcaccccac cgcagcccca
    77461 aagccgctaa agaacagcac attggcgaca gtatgcgggt cataagccag atcggttttc
    77521 agatacgttg gcagcagcgc ctgaatcggc catgagtaga ggaaagcaaa caacacgacc
    77581 accatcagca ttacgcccgt tggccagcgt ttgcccgtac tctgcaccat aaagctgata
    77641 aagattgcgg cgcataacag cccaagaaca gcgacgatcg cggcattttg caggttaccg
    77701 gcgaagcaga accacagcgc agtagccgcc gccagtgtca ttacgatatt ggcaatgcga
    77761 tgttcaccac ggtagagaat atccaccatt gtgcgtaccg gtgctttacc cccgtgtttc
    77821 tctttccagt cttccgcttc cgggatgttt ttacgcagcc agagagcaaa gatgattggc
    77881 aaaatgccga taaagaacag cgcacgccag ccccagaccg gaaccaccag gctatagacc
    77941 tgagcggcaa cgacggcccc cacagagaag cctgaaatca gaaaaccact ggctttgtta
    78001 cgcagatgtt ttggccagct ttcaatgaca taggtggcgc tggaaccgta ttcacccgcc
    78061 atccccatgc cgatgaccag acgcgcgata aacatggtga tgtagcctgg cgcaaagccg
    78121 caggccagcg tcccggccga gaagagaacg atgctggtga ccattgccag acgacgcccg
    78181 tagcggtcac ccatagcgcc gagcatcagg ccgccgaacc agcgagagat aaaggctgcg
    78241 gagatcagac ttgccgcctg caccgtcgtc agcccgaatt caccctgtac ttcggtgagt
    78301 accagggcga ttaaaacgaa atcaaaaccg tcaagcagat atcccaacca ggcagcggaa
    78361 aatgcgcgcc attgtgcacg gttgagatgg cgataccacg ggatattctg ggttgtagta
    78421 ctcattgtga gtctcccgcg gtgggcgatg cccacacgct ttggtataaa aattgtaggg
    78481 tacagatgcg tttatttccc ctcacccggt aggggcgagc gaggggaaac aactcacccg
    78541 cgctcttgca tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc
    78601 ggtccaaacg gtttgcggca cagcggcaca gaaacgacat ccatataatg gaggacagtt
    78661 ttcaggccgc ggaatacgcc cgttttgatc agtaaatcaa tgactttatt gcattcagtt
    78721 tgcagtttct gcgcggtctg gatatcgcct tctttcagcg ccttaacgat cccctgatag
    78781 cgccagccca tgatgttgta ggtactaccg ataccaccat cagcgcccgc cagcagacca
    78841 gaggcgaaga tttcgtcgta accgttatag agcacaagat caggatgttc acgacggatc
    78901 tgctccatct gatagagatc gccagaggtc tgtttcagcg cacctacgcc aggcaatgta
    78961 acaagtgtgt tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac
    79021 accaccatcg gcaaaccatc cgccgaatca ataattgccc gatagtgatc gcagtgttct
    79081 tcaaagctga aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt
    79141 ttagccgatg ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg
    79201 agtttaatct tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa
    79261 aggctttgta caaaggcctc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc
    79321 tgaatattga actgaaccag gcgacgcaga ctcgctttat ccagtgcttg ttgttgatca
    79381 aaaggagtca ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa
    79441 gtgatgcttg tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa
    79501 caccccatac agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt
    79561 gttggtggtc tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa
    79621 cgcacgatcg gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg
    79681 ttgataacta acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc
    79741 aataagccag tcgagcagag caacgtggat cgccatgaag attgggttac cggggatctc
    79801 cgccagcacg cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga
    79861 ctgactgtta atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcatgttc
    79921 agccgcatag cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat
    79981 cccaccagga tgagaaagga aatctttcgc catgccggaa agctcgccga tgatggtgtc
    80041 cgcagaagga cgtgagacgc gggcacgttc gccgttgttg atttgcacca gacctttgcg
    80101 ttttaacgct gccagcgctt cgcgcacaga aggacgcccg acgttaaaga acgccatcag
    80161 ttcgcgttca gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc
    80221 cagctcttct tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt
    80281 gcgaccaatt gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat
    80341 tctgtaaggt cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg
    80401 ggaaaatggc aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa
    80461 ctattgcgaa aaattaatgt aacgcagata aaaacatccc gtttgaatta tttataagac
    80521 tattcacgag cattatgaat attatgaatg tgttcttaca aaataatcat aagcgcatat
    80581 tttttaatga aaaatcacct cacctacaat taaaaacacg acatccgtac cataaataga
    80641 cttgcaaaaa atataacatc attgttttca atctgccgtt tatgggattg accgttttct
    80701 tttgacacgg agttcaacaa tgttcggcat aattatatct gtcatcgtat taattacgat
    80761 gggctatttg atcctgaaaa actacaaacc tcaggtggtg ctggctgccg caggtatctt
    80821 cctgatgatg tgcggtgtct ggttagggtt cggtggtgta ctcgatcccg ccaaaagcag
    80881 cggctacttg atcgtcgata tttataatga aatcctgcgc atgctgtcca accgcattgc
    80941 cggattgggg ctatcgatta tggcggtggg cggttatgcc cgctacatgg agcgcatagg
    81001 ggccagtcgc gcgatggtga gcttgttaag ccgcccgtta aaactcattc gctcgccgta
    81061 tattattctg tcggcaactt acgtcatcgg tcaaatcatg gcgcagttta ttaccagcgc
    81121 ctccggtctg ggtatgttgc tgatggtcac cttatttccg acactggtga gtctgggagt
    81181 aagtcgtctc tctgcggtgg cggttatcgc aaccacaatg tccattgagt ggggaattct
    81241 ggaaacgaac tccatttttg cagcccaggt cgcgggaatg aaaattgcca cttacttctt
    81301 ccactaccag cttccggtcg cctcttgcgt cattatctcg gtggcgatct cccacttttt
    81361 cgtgcaacgc gcttttgaca aaaaagataa aaatatcaat cacgaacaag cagagcaaaa
    81421 agctctcgat aatgtcccgc cgctctatta cgccatttta cctgtgatgc cgttaatcct
    81481 gatgctcggc tcgctgttcc tcgcccacgt cgggctgatg cagtcagaac tgcatctggt
    81541 ggtggtgatg ttactgagtt tgacggtgac gatgtttgtt gagttcttcc gcaagcataa
    81601 cttgcgcgaa acaatggacg atgtgcaggc gttttttgac ggcatgggta cgcagtttgc
    81661 caacgtggta acgctggtgg tcgcgggtga aatatttgcg aaaggcttaa cgacgattgg
    81721 cactgttgat gcggttatca ggggtgcgga gcattctggt ctgggcggta ttggcgtgat
    81781 gattattatg gcgctagtca ttgccatttg tgccattgtg atgggctctg gcaatgcgcc
    81841 gtttatgtca tttgccagtc ttattccgaa tatcgcagcc ggactacatg taccagcggt
    81901 tgtaatgatt atgccgatgc attttgccac gacgctagcg cgcgcggttt cgccgattac
    81961 tgcggtggtg gtcgttacgt caggaattgc aggcgtttcg ccttttgcgg tggtgaagcg
    82021 aacagcgatc cccatggcag tcggtttcgt ggtgaatatg attgccacaa tcacgctatt
    82081 ttattaagcc attaaaagac aaaacaggcc accagggcct gtttttgtat tacttcacaa
    82141 cgcgtaatgc cggtcgacca ccgcgtggtg gctgcggagg ttcatcgtca ggatgagtgt
    82201 catcatcgtg atctggcttg tcgccatcaa taaccgacat aacggtttcg ttgtctgccg
    82261 atgcctcttc atcattcatg atgctggtat cttcatcgta ggcagcttca ggctcaaaca
    82321 ttgtgcctgc gccattttca cgggcgtaga tagccagcac ggcagccagc ggcacagaaa
    82381 cctgacgcgg aatgccgcca aagcgcgcgt taaagcgcac ctcatcattc gccagttcca
    82441 gattgccgac agcacgcggc gcaatgttga gtacgatttg cccgtcacgc gcatattcca
    82501 taggaacctg cacgccaggg agcgtcacat ccaccaccag gtgcggcgtg agctggttat
    82561 ccagcaacca ctcatagaat gcacgcagca gatagggacg acgtggtgtt agctgtgaca
    82621 aatccataca gattaactcc ggcccagacg catttcacgt tctgcttcag ttaaagaagc
    82681 aaggaaagag tcacgctcaa agacgcgggt catatagcct ttcagctctt tcgcacccgg
    82741 gccgctgaac tcgatgccca gttgcggcag acgccacagc agcggagcaa gatagcaatc
    82801 gaccaggctg aactcatcgc tcaggaagta cggcttctga ccgaagaccg gcgcaatcgc
    82861 cagcagttct tcgcgcagtt gcttacgtgc ggcatctgct tcagaagctg aaccgttgat
    82921 gatggtgttc atcagcgtgt accagtcttt ttcgatacga tgcatgtaca gacggctttc
    82981 accgcgagct accgggtaaa caggcatcag tggcggatgc gggaaacgct catccagata
    83041 ttccataatg atgcgagatt cccacagggt cagctcacga tccaccaggg tcggaacgct
    83101 ctgattcggg ttgaggtcaa tcagatcctg aggcggattg tccttttcca cgtgttcgat
    83161 ctcgaaactt acacctttct cagccagcac aatgcggacc tgatggctat agatgtcagt
    83221 aggaccggaa aacagcgtca ttaccgaacg tttgttggca gcgacagcca tgaaaacctc
    83281 caggtatagt cagaattttt actgctacca gccaccaggt ggccagtcag aagttgtgtt
    83341 acccagtaag gaacgactct ctttgttcga aaatcaaaca aaaaatgagc aatacccgac
    83401 atttgggcag aaaattggat gatagtttac cagattttgc gacctttgtg gtgagtcgat
    83461 gccggaaatg gggaaaaaga gatgcgcttt agtctgaaat agttgactta gtcccttatt
    83521 ggcgatgtgg tttttgtttt gcctgtctgt cagacggtag caaaaagcaa ctttccaatt
    83581 tttacgctga ttcagaattt aaccataaaa aaacccgccg aagcgggttt tttcgcaaat
    83641 tgttttctgc cggagcagaa gccaattaac gtttggagaa ctgcggacga cgacgtgctt
    83701 tacgcagacc gactttctta cgttcaacct gacgagcgtc acgagtaacg aagccagctt
    83761 tacgcagttc agaacgcagg gactcgtcgt attccatcag agcgcgggtg ataccgtgac
    83821 ggatcgcacc agcctgacca gagataccac cacctttaac ggtgatgtac aggtccagtt
    83881 tctcaaccat gtcgaccagt tccagcggct gacgaactac catgcgagca gtttcacgac
    83941 cgaagtactg ttccagagaa cgttggttga ttacgatttt accgttgccc ggtttgatga
    84001 aaacgcgagc tgcggaactt ttgcggcgac cagtgccgta gtattgattt tcagccattg
    84061 cctataatcc cgattagatg tcaagaactt gcggttgctg tgccgcgtgg ttgtgctcgt
    84121 tacccgcgta aactttcagt ttacggaaca tagcacgacc cagcgggcct tttggcaaca
    84181 tgcctttaac cgcgatttca atcacacgct caggacggcg agcaatcatc tcttcaaagg
    84241 tcgcttgttt gataccaccg atgtggccgg tgtggtgata gtacactttg tcagtacgct
    84301 tgttgccggt tacagcaact ttgtcagcgt tcagaacgat gatgtaatca ccggtatcta
    84361 cgtgcggagt gtattccgct ttgtgcttac cgcgcaggcg acgagccagt tcagtagcca
    84421 gacggcccag agttttaccg gtcgcgtcaa caacatacca gtcgcgtttt acggtttctg
    84481 gtttagctgt aaaagttttc attaaaagct tacccaataa atagttacac gttggtgaac
    84541 acccaaacgt cttcaattgt tgaggttcac acgacaaagt ccggcaaacc taccccttcg
    84601 aatagcctat gccagcacac aaaaagtttt gggaaaaaaa ctttcttgta acgtggggtc
    84661 gcaggattat agagaagtcg gggtcaaaga tcgacccctt tttgtgattt gtgacaggtt
    84721 ttaacccgcc aaatgctcgc gcttcagata ctcttcgctt tgcatctctt gcagacgtga
    84781 caggcaacgc tggaactcaa acttcagccg atcgccctga taaatttcat acagcggcac
    84841 ttctgcactc accactaatt tgacatggcg ctcgtaaaac tcatccacca gcgcaataaa
    84901 gcgccgcgct tcgctctcca tcaaccgcgt cataactggt acatcaaaca acatgaccgt
    84961 atgaaagaga cgtgagagcg caatatagtc atgctgactg cgggcgtcga cgcacagcgt
    85021 agtaaaagag accgccagcg tctggttctc gacgcccatt gtcgccaatg gccgatggtt
    85081 gatttctaac gtcggtgaat tttctcgttt cgcccccgcc agcgccaacc atagtttatc
    85141 catctgcgcc cgggtttcat cgttgagtgg cgaaagccac agatgcgcct gagtgagtgt
    85201 acgcagtcga taatcaacac cagcgtccac gttcattaca tcacaatgct gtttaatggc
    85261 atcgattgca ggcagaaaac gcgcacgttg caggccattt cgataaagtt catccggcgg
    85321 aatatttgac gtcgctacca gggtaatgcc gcgggcgaac agggctttca tcagaccgcc
    85381 aagtagcatg gcgtcggtaa tatcagaaac aaaaaattcg tcaaaacaga gcacgtcagt
    85441 ttcggcttta aagcgatcgg caataatttc cagcggatcg gtctgcccct gtaaggcagt
    85501 tagctcttca tgcacccgca tcataaaacg gtgaaagtgc aggcgctgtt tccgctctcc
    85561 cggcaggctt tgatagaaaa ggtccatcag ccaggttttc ccgcgtccta caccgcccca
    85621 catatataag ccacgcactg gcgtatgctt tgtgtcttcg cgtttacccc acagcttacc
    85681 gacccgcgcc attagcccac tcgtcctggg ggctggtggc gtgctattga tgagttcctg
    85741 ataaataatt tccaggcggc tgacggcctc tttttgaacg tcgtcgggtt gatggctgcc
    85801 ttcattgagc gccttcaggt attgcgatgt tggggtaacg ctttgcatga tcttattgtt
    85861 attccttgaa taatcggtgc gccgttgttc acggttgacg aaaaaaaggc cgttctacac
    85921 tacgcgatat gcagtcggga ttccacttct gtggaattaa cggttatagt ggcataatca
    85981 gccgcaggca tggagcctga agccaacacc ctacggaaac aaaagacaac gggagatgtt
    86041 catgacctgg gaatatgcgc taattgggtt agtcgtcggc atcattattg gtgctgtggc
    86101 catgcgtttt ggtaatcgta aactacgcca gcaacaggcg ttgcagtacg aactggaaaa
    86161 gaataaagct gaactggacg agtatcgcga agagctggtt agccactttg cccgcagcgc
    86221 ggaattactg gataccatgg cgcacgacta tcgccagctg tatcagcaca tggcgaaaag
    86281 ctccagcagc ctgctgccgg aactgtctgc tgaagcaaac ccgttccgta atcgtctggc
    86341 agagtctgaa gccagcaacg atcaggcacc ggtgcagatg cctcgcgact attctgaagg
    86401 cgcatccggc ctgctgcgta ctggcgcgaa gcgcgactaa tttatttttc gggcgcagcc
    86461 attgcgccct cctcttctct ccctccccga ctatcattta atctggtgtc tcattgttag
    86521 ccgtctgaaa attcaataac atcaaactgt tttgaatctc ttttcttatc attcaggtac
    86581 gagagcagga ataatgaaaa aacaaaccca gctgttgagt gcattagcgt taagtgtcgg
    86641 gttaactctc tcggcgtcat ttcaggccgt cgcgtcgatt ccaggccagg ttgccgatca
    86701 ggcccctctc cccagtctgg ctccaatgct ggaaaaagtg cttccggcag tggtgagcgt
    86761 acgggtggaa ggaacggcca gtcagggaca gaaaatcccg gaagaattca aaaagttttt
    86821 tggtgatgat ttaccggatc aacctgcaca acccttcgaa ggtttaggtt ccggtgtcat
    86881 catcaacgcc agtaaaggct atgtgctgac caacaaccat gtgattaatc aggcacagaa
    86941 aatcagtatt cagctcaatg atgggcgcga gtttgatgca aaactgattg gtagcgatga
    87001 ccagagcgat atcgccctgt tacaaattca aaacccgagc aaattaacgc aaatcgctat
    87061 tgccgactcc gataaattgc gcgtcggtga ttttgccgta gcggtcggta acccatttgg
    87121 ccttgggcaa accgccacct ctggcattgt ttccgcatta ggacgcagcg ggttgaatct
    87181 tgaaggtctg gaaaacttta tccagacaga tgcttccatt aaccgcggta actccggcgg
    87241 tgcactgtta aaccttaacg gtgagttaat tggcatcaac actgcaatcc ttgcgcctgg
    87301 cggcgggagc gtcgggattg gatttgccat ccccagtaat atggcgcgaa cactggcgca
    87361 gcagcttatc gactttggtg aaatcaaacg cggtttgtta ggcatcaaag gcaccgagat
    87421 gagtgccgat atcgccaaag ccttcaacct tgacgtgcag cgtggcgcgt ttgtcagcga
    87481 agtgttgcca ggttctggct cagcgaaagc gggcgtcaaa gcgggcgata ttattaccag
    87541 cctcaacggc aaaccgctga atagctttgc tgagttgcgc tctcgtatcg cgaccaccga
    87601 gccgggcacg aaagtgaagc ttggcctgct gcgtaacggc aagccactgg aagtagaagt
    87661 gacgctcgat accagcacct cttcgtcggc cagcgctgaa atgatcacgc cagcgctgga
    87721 aggtgcaacg ttgagcgatg gtcagctaaa agatggcggc aaaggtatta agatcgatga
    87781 agttgtcaaa ggaagcccag ctgctcaggc tggcttgcaa aaagacgatg tgatcattgg
    87841 cgtcaaccgc gatcgggtga actcgattgc tgaaatgcgt aaagtgctgg cggcaaaacc
    87901 ggccatcatc gccctgcaaa ttgtacgcgg caatgaaagc atctatctgc tgatgcgtta
    87961 atgtcgtaaa ccgggcatca ggcttacgtg tgatgtccgg ttaactcgtg gtatgctgct
    88021 gccgttccct tttttaatga cgcctccatc atgtttgtga agctcttacg ttccgttgcg
    88081 attggattaa ttgtcggcgc tattctgctg gttgccatgc cttcgctgcg cagccttaac
    88141 ccgctttcca ctccgcaatt tgacagtacc gatgagacgc ctgccagcta taatctggcg
    88201 gttcgccgcg ccgcgccagc ggtggttaac gtttacaacc gtggtttgaa caccaactct
    88261 cacaaccagc ttgagatccg caccctggga tccggtgtaa tcatggatca acgcggttat
    88321 atcatcacca ataaacacgt catcaacgac gccgatcaga tcatcgtcgc cttacaggat
    88381 ggtcgtgtat ttgaagcatt gctggtggga tctgactctc taaccgatct ggcggtactt
    88441 aaaattaatg ccactggcgg tttacctacc attccgatca atgcacgtcg cgtaccgcac
    88501 attggcgacg tagtactggc gatcggtaac ccgtacaacc tcgggcagac cattacccag
    88561 gggattatta gtgccacggg tcgaatcggt ctgaacccga ccgggcggca aaacttccta
    88621 caaaccgatg cttccattaa ccacggtaac tctggcggcg cgctggtgaa ctcgctgggc
    88681 gaactgatgg gcattaacac gctgtcgttt gataagagta acgatggcga aacgccggaa
    88741 ggtatcggct ttgcgattcc tttccagtta gcaaccaaaa ttatggataa gctgatccgc
    88801 gatggtcgcg tgatccgcgg ctacattggt atcggcggac gcgagatcgc accactgcac
    88861 gcgcagggcg gtggtataga tcaactgcaa gggatcgtgg ttaatgaagt gtcacctgac
    88921 ggcccggcgg cgaatgcggg tattcaggtc aacgatctga ttatttcggt ggataacaaa
    88981 ccggccatct ctgctctgga gacgatggat caggtggcgg aaattcgccc tggttcggtg
    89041 atcccggttg tagtgatgcg tgatgataag cagttaacgc tgcaggtcac cattcaggaa
    89101 tatccggcaa ccaattaagt cgagtgctca aaacaaaaac cggagtctgt gctccggttt
    89161 tttattattc gctaatcaat tacttattaa cgaactcttc gcccagggcg atatctttct
    89221 tgagcgtatc cagcatacct tccagcgcgt tctgttcaaa tgcgctcagg gtaccgatag
    89281 atttacgctc ttccacgccg tttttaccca gcagcagcgg ttgagagaag aaacgggcgt
    89341 actgaccgtc gccttcaacg taggcacatt cgacaacgcc ttgttcgccc tgcagtgcac
    89401 gaaccagaga cagaccaaaa cgtgcagctg cctggcccat agacagggtt gcagacccgc
    89461 caccggcttt cgcttcaacc acctcagtac ccgcgttctg gatacgtttg gtcagatcag
    89521 ccacttcctg ctcggtaaaa ctaacgctag gaacctgtga cagcagcggc agaatggtaa
    89581 caccagagtg accgccaata accggcactt caacttcgcc tggctgtttg cctttcagtt
    89641 ccgcaacaaa agtgttggaa cgaatgatat ccagcgtggt aacgccgaac agtttgtttt
    89701 tgtcataaac accggctttt ttcagcactt cagcagcaat cgcaactgtg gtgttaaccg
    89761 ggttagtgat aataccaatg cacgctttcg ggcaggtttt cgcaacttgc tgtaccaggt
    89821 ttttcacgat gccggcgtta acgttaaaca ggtcggaacg atccataccc ggtttacgcg
    89881 ctacacctgc agagataaga acgacatctg cgccttccag cgccggagtc gcatcttcac
    89941 cagaaaaacc tttgattttc acagcagtag ggatatggct cagatcgacg gccacaccgg
    90001 gagtcactgg agcgatatca tacagagaga gttctgaacc tgaaggcagt tgggttttta
    90061 acagtagtgc aagcgcctgg ccaataccgc cagcagcgcc gaggactgcg actttcatcc
    90121 taaactcctt attatattga taaactaaga tatgttgctc cgctgccgcg accttaatcc
    90181 acaaaattgc cgtttacaat gaccacatct caagaatgtg tagtcacgca agtttagcgt
    90241 ttatgcattt aattgccgta atcaggaacc taacttacgt aattaacagt cgcttatgca
    90301 ttagcgcaac attccaacag gtggtgacaa tataccctac cgttcagcca aaacaacatc
    90361 aatttgataa caattaattt acttttaagc agaatttgca tgccgtgacg caggcatgtt
    90421 tctcaataac gaaatttgat aaaatcccgc tctttcataa cattatttca gccttcttca
    90481 gggctgactg tttgcataaa aattcatctg tatgcacaat aatgttgtat caaccaccat
    90541 atcgggtgac ttatgcgaag ctcggctaag caagaagaac tagttaaagc atttaaagca
    90601 ttacttaaag aagagaaatt tagctcccag ggcgaaatcg tcgccgcgtt gcaggagcaa
    90661 ggctttgaca atattaatca gtctaaagtc tcgcggatgt tgaccaagtt tggtgctgta
    90721 cgtacacgca atgccaaaat ggaaatggtt tactgcctgc cagctgaact gggtgtacca
    90781 accacctcca gtccattgaa aaatctggtg ctggatatcg actacaacga tgcagttgtc
    90841 gtgattcata ccagccctgg cgcggcgcag ttaattgctc gcctgctgga ctcactgggc
    90901 aaagcagaag gtattctggg caccatcgct ggcgatgaca ccatctttac cacccctgct
    90961 aacggtttca ccgtcaaaga cctgtacgaa gcgattttag agctgttcga ccaggagctt
    91021 taatctctgc cccgtcgctt ctggcggcgg ggaaaatgtt gcttatccct ctcaaccccc
    91081 tgctttctcc tgcgatacat ttaacgaata gtgcgtttta ctgcgacatg tcattcacac
    91141 aatgaataca taaggtaaaa aaagcac
//
LOCUS       DAJOWB010000005        87284 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJOWB010000005 DAJOWB010000000
VERSION     DAJOWB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 87284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.005"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..529
                     /gene="zapC"
                     /locus_tag="OHO85_000388"
     CDS             <1..529
                     /gene="zapC"
                     /locus_tag="OHO85_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCQ2669819.1"
                     /translation="DDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAFSPAGF
                     CVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHGEMWVP
                     MPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKIMNDRL
                     KPQVNVDSFSLEQAV"
     gene            complement(526..1635)
                     /gene="ycbX"
                     /locus_tag="OHO85_000389"
     CDS             complement(526..1635)
                     /gene="ycbX"
                     /locus_tag="OHO85_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCQ2669820.1"
                     /translation="MVTLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            1879..3987
                     /gene="rlmKL"
                     /locus_tag="OHO85_000390"
     CDS             1879..3987
                     /gene="rlmKL"
                     /locus_tag="OHO85_000390"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCQ2669821.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            3999..5906
                     /locus_tag="OHO85_000391"
     CDS             3999..5906
                     /locus_tag="OHO85_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2669822.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAVPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6036..7289
                     /gene="pqiA"
                     /locus_tag="OHO85_000392"
     CDS             6036..7289
                     /gene="pqiA"
                     /locus_tag="OHO85_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCQ2669823.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7294..8934
                     /gene="pqiB"
                     /locus_tag="OHO85_000393"
     CDS             7294..8934
                     /gene="pqiB"
                     /locus_tag="OHO85_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCQ2669824.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            8931..9494
                     /gene="pqiC"
                     /locus_tag="OHO85_000394"
     CDS             8931..9494
                     /gene="pqiC"
                     /locus_tag="OHO85_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCQ2669825.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            9750..9917
                     /gene="rmf"
                     /locus_tag="OHO85_000395"
     CDS             9750..9917
                     /gene="rmf"
                     /locus_tag="OHO85_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCQ2669826.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(9987..10505)
                     /gene="fabA"
                     /locus_tag="OHO85_000396"
     CDS             complement(9987..10505)
                     /gene="fabA"
                     /locus_tag="OHO85_000396"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCQ2669827.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10574..12334)
                     /locus_tag="OHO85_000397"
     CDS             complement(10574..12334)
                     /locus_tag="OHO85_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCQ2669828.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRAFSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12520..12972
                     /gene="matP"
                     /locus_tag="OHO85_000398"
     CDS             12520..12972
                     /gene="matP"
                     /locus_tag="OHO85_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCQ2669829.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13048..14100)
                     /gene="ompA"
                     /locus_tag="OHO85_000399"
     CDS             complement(13048..14100)
                     /gene="ompA"
                     /locus_tag="OHO85_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCQ2669830.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKH
                     FTLKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQG
                     LSERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRV
                     EIEVKGIKDVVTQPQA"
     gene            complement(14457..14966)
                     /gene="sulA"
                     /locus_tag="OHO85_000400"
     CDS             complement(14457..14966)
                     /gene="sulA"
                     /locus_tag="OHO85_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCQ2669831.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15185..15814
                     /gene="sxy"
                     /locus_tag="OHO85_000401"
     CDS             15185..15814
                     /gene="sxy"
                     /locus_tag="OHO85_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCQ2669832.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15777..17939)
                     /gene="yccS"
                     /locus_tag="OHO85_000402"
     CDS             complement(15777..17939)
                     /gene="yccS"
                     /locus_tag="OHO85_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCQ2669833.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(17949..18395)
                     /locus_tag="OHO85_000403"
     CDS             complement(17949..18395)
                     /locus_tag="OHO85_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCQ2669834.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            18518..20572
                     /gene="helD"
                     /locus_tag="OHO85_000404"
     CDS             18518..20572
                     /gene="helD"
                     /locus_tag="OHO85_000404"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCQ2669835.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(20604..21062)
                     /gene="mgsA"
                     /locus_tag="OHO85_000405"
     CDS             complement(20604..21062)
                     /gene="mgsA"
                     /locus_tag="OHO85_000405"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCQ2669836.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(21158..21820)
                     /locus_tag="OHO85_000406"
     CDS             complement(21158..21820)
                     /locus_tag="OHO85_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCQ2669837.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            21993..22406
                     /locus_tag="OHO85_000407"
     CDS             21993..22406
                     /locus_tag="OHO85_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCQ2669838.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(22451..22768)
                     /gene="hspQ"
                     /locus_tag="OHO85_000408"
     CDS             complement(22451..22768)
                     /gene="hspQ"
                     /locus_tag="OHO85_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCQ2669839.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(22826..24016)
                     /gene="rlmI"
                     /locus_tag="OHO85_000409"
     CDS             complement(22826..24016)
                     /gene="rlmI"
                     /locus_tag="OHO85_000409"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCQ2669840.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            24111..24389
                     /gene="yccX"
                     /locus_tag="OHO85_000410"
     CDS             24111..24389
                     /gene="yccX"
                     /locus_tag="OHO85_000410"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCQ2669841.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELADFRIR"
     gene            complement(24386..24715)
                     /gene="tusE"
                     /locus_tag="OHO85_000411"
     CDS             complement(24386..24715)
                     /gene="tusE"
                     /locus_tag="OHO85_000411"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCQ2669842.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(24806..25465)
                     /gene="yccA"
                     /locus_tag="OHO85_000412"
     CDS             complement(24806..25465)
                     /gene="yccA"
                     /locus_tag="OHO85_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCQ2669843.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(25672..25759)
                     /locus_tag="OHO85_000413"
     tRNA            complement(25672..25759)
                     /locus_tag="OHO85_000413"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25723..25725),aa:Ser,seq:tga)
     gene            26186..27304
                     /gene="hyaA"
                     /locus_tag="OHO85_000414"
     CDS             26186..27304
                     /gene="hyaA"
                     /locus_tag="OHO85_000414"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCQ2669844.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGNQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            27301..29094
                     /gene="hyaB"
                     /locus_tag="OHO85_000415"
     CDS             27301..29094
                     /gene="hyaB"
                     /locus_tag="OHO85_000415"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCQ2669845.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            29113..29820
                     /gene="hyaC"
                     /locus_tag="OHO85_000416"
     CDS             29113..29820
                     /gene="hyaC"
                     /locus_tag="OHO85_000416"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCQ2669846.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            29817..30404
                     /locus_tag="OHO85_000417"
     CDS             29817..30404
                     /locus_tag="OHO85_000417"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCQ2669847.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            30401..30799
                     /gene="hyaE"
                     /locus_tag="OHO85_000418"
     CDS             30401..30799
                     /gene="hyaE"
                     /locus_tag="OHO85_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCQ2669848.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            30796..31653
                     /locus_tag="OHO85_000419"
     CDS             30796..31653
                     /locus_tag="OHO85_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCQ2669849.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            31787..33331
                     /gene="appC"
                     /locus_tag="OHO85_000420"
     CDS             31787..33331
                     /gene="appC"
                     /locus_tag="OHO85_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCQ2669850.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFALKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            33343..34479
                     /gene="appB"
                     /locus_tag="OHO85_000421"
     CDS             33343..34479
                     /gene="appB"
                     /locus_tag="OHO85_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCQ2669851.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARDDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            34492..34584
                     /gene="cbdX"
                     /locus_tag="OHO85_000422"
     CDS             34492..34584
                     /gene="cbdX"
                     /locus_tag="OHO85_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCQ2669852.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            34664..35962
                     /gene="appA"
                     /locus_tag="OHO85_000423"
     CDS             34664..35962
                     /gene="appA"
                     /locus_tag="OHO85_000423"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCQ2669853.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGAIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(36082..38262)
                     /gene="etk"
                     /locus_tag="OHO85_000424"
     CDS             complement(36082..38262)
                     /gene="etk"
                     /locus_tag="OHO85_000424"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCQ2669854.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGINVYATIPMSEWLDKRSRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(38282..38728)
                     /gene="etp"
                     /locus_tag="OHO85_000425"
                     /gene_synonym="yccY"
     CDS             complement(38282..38728)
                     /gene="etp"
                     /locus_tag="OHO85_000425"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCQ2669855.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(38716..39855)
                     /locus_tag="OHO85_000426"
     CDS             complement(38716..39855)
                     /locus_tag="OHO85_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCQ2669856.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(39901..41997)
                     /locus_tag="OHO85_000427"
     CDS             complement(39901..41997)
                     /locus_tag="OHO85_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCQ2669857.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGHWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREIPVGQ"
     gene            complement(41997..42743)
                     /locus_tag="OHO85_000428"
     CDS             complement(41997..42743)
                     /locus_tag="OHO85_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCQ2669858.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(42740..43384)
                     /locus_tag="OHO85_000429"
     CDS             complement(42740..43384)
                     /locus_tag="OHO85_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCQ2669859.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSHWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(43491..43796)
                     /locus_tag="OHO85_000430"
     CDS             complement(43491..43796)
                     /locus_tag="OHO85_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2669860.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(44238..44450)
                     /gene="cspH"
                     /locus_tag="OHO85_000431"
     CDS             complement(44238..44450)
                     /gene="cspH"
                     /locus_tag="OHO85_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCQ2669861.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            44736..44948
                     /gene="cspG"
                     /locus_tag="OHO85_000432"
     CDS             44736..44948
                     /gene="cspG"
                     /locus_tag="OHO85_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCQ2669862.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            44959..45147
                     /locus_tag="OHO85_000433"
     CDS             44959..45147
                     /locus_tag="OHO85_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCQ2669863.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            45122..45352
                     /gene="ymcE"
                     /locus_tag="OHO85_000434"
     CDS             45122..45352
                     /gene="ymcE"
                     /locus_tag="OHO85_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCQ2669864.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            45342..45515
                     /locus_tag="OHO85_000435"
     CDS             45342..45515
                     /locus_tag="OHO85_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCQ2669865.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(45564..46637)
                     /locus_tag="OHO85_000436"
     CDS             complement(45564..46637)
                     /locus_tag="OHO85_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCQ2669866.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHVAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(46709..49453)
                     /gene="torS"
                     /locus_tag="OHO85_000437"
     CDS             complement(46709..49453)
                     /gene="torS"
                     /locus_tag="OHO85_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCQ2669867.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITIEMLKTSGAQIVAVGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            49536..50564
                     /gene="torT"
                     /locus_tag="OHO85_000438"
     CDS             49536..50564
                     /gene="torT"
                     /locus_tag="OHO85_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCQ2669868.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(50537..51229)
                     /gene="torR"
                     /locus_tag="OHO85_000439"
     CDS             complement(50537..51229)
                     /gene="torR"
                     /locus_tag="OHO85_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCQ2669869.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            51359..52531
                     /gene="torC"
                     /locus_tag="OHO85_000440"
     CDS             51359..52531
                     /gene="torC"
                     /locus_tag="OHO85_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCQ2669870.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDMPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            52531..55077
                     /gene="torA"
                     /locus_tag="OHO85_000441"
     CDS             52531..55077
                     /gene="torA"
                     /locus_tag="OHO85_000441"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HCQ2669871.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            55074..55673
                     /gene="torD"
                     /locus_tag="OHO85_000442"
     CDS             55074..55673
                     /gene="torD"
                     /locus_tag="OHO85_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCQ2669872.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(55766..56071)
                     /gene="cbpM"
                     /locus_tag="OHO85_000443"
     CDS             complement(55766..56071)
                     /gene="cbpM"
                     /locus_tag="OHO85_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCQ2669873.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(56071..56991)
                     /gene="cbpA"
                     /locus_tag="OHO85_000444"
     CDS             complement(56071..56991)
                     /gene="cbpA"
                     /locus_tag="OHO85_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCQ2669874.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            57251..58510
                     /locus_tag="OHO85_000445"
     CDS             57251..58510
                     /locus_tag="OHO85_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCQ2669875.1"
                     /translation="MSSNIHGISCTANNYLKQAWNNIKNEHEKNQKYSITLFENTLVC
                     FMRLYKEIRRQKAEDYIPCLECDSLEKEFEEMQNDNDLSLFLRTLRTNDTETYSGVSE
                     GITYTIQYVRDIDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEWINTPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFNDLREDVKTLFGVNLQS
                     DNKIQGQGGFLSWSFLSFKKEFIDSCLNDKKCILHLPEFIFNDNKARLVLDTDPEQKV
                     NEVKNFLTALSDSICSLFIVNSNVASISLGNESFSTDDDLEYGYLINTGNHYDVYLPP
                     ELFAQAYELNNKERNAQIDFLTRYAIY"
     gene            58802..60043
                     /gene="agp"
                     /locus_tag="OHO85_000446"
     CDS             58802..60043
                     /gene="agp"
                     /locus_tag="OHO85_000446"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCQ2669876.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(60081..60308)
                     /locus_tag="OHO85_000447"
     CDS             complement(60081..60308)
                     /locus_tag="OHO85_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCQ2669877.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(60329..60925)
                     /gene="wrbA"
                     /locus_tag="OHO85_000448"
     CDS             complement(60329..60925)
                     /gene="wrbA"
                     /locus_tag="OHO85_000448"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCQ2669878.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            61298..61471
                     /locus_tag="OHO85_000449"
     CDS             61298..61471
                     /locus_tag="OHO85_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCQ2669879.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(61554..62882)
                     /gene="rutG"
                     /locus_tag="OHO85_000450"
     CDS             complement(61554..62882)
                     /gene="rutG"
                     /locus_tag="OHO85_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCQ2669880.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAIL
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(62903..63397)
                     /gene="rutF"
                     /locus_tag="OHO85_000451"
     CDS             complement(62903..63397)
                     /gene="rutF"
                     /locus_tag="OHO85_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCQ2669881.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(63408..63998)
                     /locus_tag="OHO85_000452"
     CDS             complement(63408..63998)
                     /locus_tag="OHO85_000452"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCQ2669882.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(64008..64808)
                     /gene="rutD"
                     /locus_tag="OHO85_000453"
     CDS             complement(64008..64808)
                     /gene="rutD"
                     /locus_tag="OHO85_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCQ2669883.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARVPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(64816..65202)
                     /gene="rutC"
                     /locus_tag="OHO85_000454"
     CDS             complement(64816..65202)
                     /gene="rutC"
                     /locus_tag="OHO85_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCQ2669884.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(65214..65906)
                     /gene="rutB"
                     /locus_tag="OHO85_000455"
     CDS             complement(65214..65906)
                     /gene="rutB"
                     /locus_tag="OHO85_000455"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCQ2669885.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(65906..66997)
                     /gene="rutA"
                     /locus_tag="OHO85_000456"
     CDS             complement(65906..66997)
                     /gene="rutA"
                     /locus_tag="OHO85_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCQ2669886.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            67285..67923
                     /gene="rutR"
                     /locus_tag="OHO85_000457"
     CDS             67285..67923
                     /gene="rutR"
                     /locus_tag="OHO85_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCQ2669887.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(67963..71925)
                     /gene="putA"
                     /locus_tag="OHO85_000458"
     CDS             complement(67963..71925)
                     /gene="putA"
                     /locus_tag="OHO85_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCQ2669888.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            72347..73855
                     /gene="putP"
                     /locus_tag="OHO85_000459"
     CDS             72347..73855
                     /gene="putP"
                     /locus_tag="OHO85_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCQ2669889.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(74060..74362)
                     /locus_tag="OHO85_000460"
     CDS             complement(74060..74362)
                     /locus_tag="OHO85_000460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2669890.1"
                     /translation="MQRKVNRSDSDYHYGKEKYKRNVYKMMLECKQREVMPRDINDYS
                     RVERWDCLQRTMCSCRMPRKRRIQPTKLSRLNASELPCKTDKRVYQAISSIRDYQI"
     gene            74398..75228
                     /locus_tag="OHO85_000461"
     CDS             74398..75228
                     /locus_tag="OHO85_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCQ2669891.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            75286..76413
                     /gene="efeO"
                     /locus_tag="OHO85_000462"
     CDS             75286..76413
                     /gene="efeO"
                     /locus_tag="OHO85_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCQ2669892.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            76419..77690
                     /gene="efeB"
                     /locus_tag="OHO85_000463"
     CDS             76419..77690
                     /gene="efeB"
                     /locus_tag="OHO85_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCQ2669893.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            78309..79097
                     /gene="phoH"
                     /locus_tag="OHO85_000464"
     CDS             78309..79097
                     /gene="phoH"
                     /locus_tag="OHO85_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCQ2669894.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIIRFGKEDCVRSALCQRTLHAYS"
     gene            complement(79147..79560)
                     /gene="pgaD"
                     /locus_tag="OHO85_000465"
     CDS             complement(79147..79560)
                     /gene="pgaD"
                     /locus_tag="OHO85_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HCQ2669895.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(79562..80887)
                     /gene="pgaC"
                     /locus_tag="OHO85_000466"
     CDS             complement(79562..80887)
                     /gene="pgaC"
                     /locus_tag="OHO85_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HCQ2669896.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDRTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            complement(80880..82898)
                     /gene="pgaB"
                     /locus_tag="OHO85_000467"
     CDS             complement(80880..82898)
                     /gene="pgaB"
                     /locus_tag="OHO85_000467"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HCQ2669897.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSKTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADEQVKFGDEMVD
                     REYFATWQQVREVARSPLVEVASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKAKVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANSSQLDSIPRVLIANNPSLKEFAQQIITVQEKSSQRIMHIDLDYVYDENRQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYRRLSPFDDKVRAQVGMLY
                     EDLAGHAAFDGLLFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWTR
                     FKSRALTDFTLELSARVKAIRGPHVKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYMEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHDQPEIDVIRPEFSTAWYPKND"
     gene            complement(82907..85330)
                     /gene="pgaA"
                     /locus_tag="OHO85_000468"
                     /pseudo
     CDS             complement(82907..85330)
                     /gene="pgaA"
                     /locus_tag="OHO85_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
     gene            85917..87275
                     /locus_tag="OHO85_000469"
     CDS             85917..87275
                     /locus_tag="OHO85_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCQ2669898.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCVLSLAFLSCLIYFVKTVIIIQQIIEER
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFG
                     GPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNIAHRDPLTNIFNRNYFFNELTVQSASAQKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
BASE COUNT        21473 a        21720 c        22737 g        21354 t
ORIGIN      
        1 agacgataac tggcgttggt attacgatga agagcacgat cgaatgatgc tcgatttagc
       61 caatggtatg ctatttcgct cacgttttgc gcgcaagatg ttgactccag atgccttttc
      121 tcccgcaggc ttttgcgttg acgacgccgc gctctatttc tcttttgaag aaaagtgccg
      181 cgattttaat ttatctaaag aacaaaaagc cgaactggtg ctgaatgcac tggtagcaat
      241 tcgctatctc aaaccgcaaa tgccgaaaag ctggcatttt gtttcccatg gtgaaatgtg
      301 ggtgcccatg ccgggcgatg cggcctgcgt ttggttgagc gatactcacg agcaggtcaa
      361 tttgctggtc gttgaatctg gcgaaaatgc cgcactatgc ctgctggcac aaccctgcgt
      421 tgttattgcg ggtcgcgcga tgcagttggg cgacgccatt aaaatcatga acgacaggct
      481 gaaaccgcag gttaatgttg acagcttcag cctcgaacag gcagtctaac gcgccaactt
      541 aagtgcagtc ttcggtacac agctacagca aagaatggtg ccatcatcgc ccattgctga
      601 ttttttcagc ggcgtgactt cgccttctaa aagctgaaca cggcaacttc cacaaatgcc
      661 cgcgcggcaa gaataaggga tacgaattcc ctgattttct aattgctcca gcaacacctg
      721 ttggttattt ccacgaaatg cctgtccctg ccagtcaata tctacatttg cgtccggttg
      781 ttgcgtgatg ttggcggtat catcagcggc agctgcgccg taaatttttg ccggagccgt
      841 tgccagaatt tccacctcat cgccaacgcg aatcacgccg ctattacggg caattaaatt
      901 ctgaccaaaa tcgacatcgc cgttatcctg ggcagtgcgg aaagattgca atgtttttaa
      961 tggttcgcct gccggatgtt tttgcccttt ttctgggctg acggtggtga aaatacagcg
     1021 gctacaaggt ttaaccacat caaacaccac atcaccaatg cgaatcactt tccagcgatc
     1081 ttcttcccag gctgacgcgc cggaaaccac cagattgggg cggaattgct ccatttttac
     1141 actggccgga caacgttgtt ggagatcacg taacgaggct tcgttagcaa gaaggtaagg
     1201 atagccatca gcaaatgaca gaggtacagt gttgtggcgt ttcacgcgcc gggtcatttg
     1261 tggccccacc cagcgtaatt gcacttcgcg ggagaaaaat ccacttagcc atttgttgat
     1321 cgcgtctggc gcaattcgcg cggtaaaatg tgtgccccaa acttcggttg gtgcgtcttg
     1381 tgtggcgaaa tcagcaaaac gaacatatgc gctactgcca tctggtgcgg tgagatgcaa
     1441 gccatcatgc acaggtgaag gagtaaaccg taccatctgg ggaaactggc gagcggtaat
     1501 aaacgtaccg tcaggttccg tgatcataaa gatgcgatcg aaggccagac cactgacatc
     1561 agccagagca tgtgtaagac caatgccgcg catcgattta acaggatgaa taaaaagccg
     1621 ggttaatgtc accacggtgc ggtcctcaaa tgaaaataag ccctcaactt tatgacatga
     1681 cgcgcttatt agctataatg cgcaacaatt ttcttagcgc ctgtcccatt aggctatttt
     1741 atttgtcagt ttggccctgg gttgtgctcg aaatcctcac gtacttgtgt acgctccggt
     1801 ttctccgcgc tgtccatgtc cagactgact gcaacaatta cgcctactgc ggtaggctct
     1861 tagagtaaaa gtgacgatat gaattctctg tttgccagta cggcccgtgg gctggaagag
     1921 ctgttaaaaa ctgaactgga aaacctgggg gccgttgaat gccaggtggt tcagggtggg
     1981 gtccatttca agggcgacac acggcttgtt taccagagcc tgatgtggag ccgcctggcc
     2041 tcgcgtatta tgttgccgct gggcgagtgt aaggtttaca gcgatttaga cctctatctc
     2101 ggtgttcagg cgatcaactg gacagagatg tttaatcctg gcgcgacctt cgctgtccac
     2161 ttcagtggtt tgaatgacac catccgcaac agtcagtacg gcgcgatgaa agtgaaagac
     2221 gcgatcgtcg atgctttcac gcggaaaaat ctgccgcgtc caaatgttga tcgcgatgcg
     2281 ccggatatcc gcgttaacgt ctggctgcat aaagaaaccg ccagtatcgc ccttgatctc
     2341 agtggtgatg gtttacatct gcgtggctat cgcgatcgtg ctggtattgc gccgatcaaa
     2401 gaaaccctgg cagccgctat tgtgatgcga tccggctggc agccaggaac accgctgctc
     2461 gatccgatgt gtggttccgg tacgttgctg attgaagcag cgatgctggc gaccgatcgc
     2521 gcacctggct tgcaccgtgg gcgttggggc tttagcggct gggcgcagca tgatgaagct
     2581 atctggcagg aagtgaaagc ggaagcgcaa actcgcgccc gtaaaggcct ggctgagtat
     2641 agctctcatt tttacggttc ggacagcgac gcacgggtga ttcaacgtgc gcgcactaac
     2701 gcccgtcttg cggggattgg tgaactgatc acctttgagg tgaaagatgt cgcgcaactg
     2761 accaatccgc tgccgaaagg gccgtacggt acagtgttga gcaacccgcc atacggtgaa
     2821 cgtctggaca gcgaaccggc gctgatagcg ctgcatagtc tgctgggccg aatcatgaaa
     2881 aaccagttcg gtggctggaa tctctctttg tttagtgcct cgccggatct gctcagctgc
     2941 ctgcagctgc gtgcagacaa acagtacaag gcgaaaaacg gcccgctgga ctgcgtacag
     3001 aaaaattacc acgttgccga aagcactcca gacagcaaac cggcgatggt agcggaagac
     3061 tacgctaacc gcctgcgtaa gaacctcaaa aaattcgaga agtgggctcg ccaggaaggg
     3121 attgaatgtt accgcctgta tgacgccgat ctgccggaat ataacgttgc cgttgaccgt
     3181 tatgccgact gggtggtggt gcaggagtat gcgccgccaa aaactattga tgcgcacaaa
     3241 gcgcgtcagc gtctgttcga tattatcgct gcaaccattt cggtactggg gattgcgcca
     3301 aacaaactgg tgctgaaaac ccgtgaacgc cagaagggca aaaatcagta ccagaaactg
     3361 ggcgagaagg gcgagtttct cgaagtcacc gaatataacg ctcacttgtg ggtgaacctg
     3421 acggattacc tcgataccgg tctgttcctc gatcaccgta tcgcccgtcg tatgctcggt
     3481 cagatgagca aaggcaaaga tttcctcaac ctgttctctt ataccggcag cgccaccgtg
     3541 cacgcgggat taggcggtgc acgcagcacc accaccgtgg atatgtcgcg tacttatctg
     3601 gagtgggcag aacgcaacct gcgtctgaat ggcctaaccg ggcgtgcgca tcgcctgatt
     3661 caggccgatt gcctggcgtg gctgcgtgag gcaaatgaac agttcgatct gatctttatc
     3721 gatccgccaa ccttctctaa ctcaaaacga atggaagatg cgtttgatgt tcagcgcgat
     3781 catctggcgc tgatgaaaga tttgaaacgt ctgctgcgtg ctggtgggac gatcatgttc
     3841 tcgaacaaca aacgtggctt ccgtatggat ctcgacggcc tggcaaaact gggactgaaa
     3901 gcacaagaaa ttacgcaaaa aacgctctcc caggatttcg cccgtaaccg ccagatccac
     3961 aactgctggc tgattaccgc agcctgaaag gaatagtaat gtcattaatc agtatgcatg
     4021 gcgcatggct gtcgttcagc gacgcgccgc ttctcgataa cgcagaactg catatcgaag
     4081 ataacgaacg tgtttgtctg gtgggccgca acggcgcagg caaatcgacg ttaatgaaaa
     4141 tcctcaaccg tgaacaaggg ctggatgacg gtcgcattat ttacgagcag gatttgattg
     4201 tagcgcgtct gcaacaggat ccgccgcgta acgttgaggg tagtgtttat gatttcgttg
     4261 ccgaaggcat tgaagaacaa gcggaatatc tgaaacgcta tcacgatatt tctcgcctgg
     4321 tgatgaacga cccgagcgag aaaaatctca acgaactggc aaaggttcag gaacagctgg
     4381 atcaccacaa cctgtggcag ctggaaaacc gcatcaacga agtgctggcg caactggggt
     4441 tagatcctaa cgttgcgctg tcgtcgcttt ccggcggctg gttgcgtaaa gcggcattag
     4501 gacgcgcgct ggtgagcaat ccgcgcgtat tgctgctgga cgaaccgact aaccacctgg
     4561 atatcgaaac catcgactgg ctggaagggt tcctcaaaac cttcaatggg acgattattt
     4621 tcatctccca cgaccgttcg tttatccgca atatggcgac gcgcattgtt gatctcgatc
     4681 gcggcaagct ggtgacctat ccgggcaatt acgatcagta cctgctggaa aaagaagaag
     4741 ccctgcgcgt ggaagaacta caaaatgccg agttcgatcg caaactggcg caggaagaag
     4801 tgtggattcg ccaggggatc aaagcacgcc gtacccgtaa tgaaggccgc gtacgcgccc
     4861 tgaaagcgat gcgtcgcgag cggggcgaac gtcgcgaagt gatgggtacc gcaaagatgc
     4921 aggtggaaga ggccagccgc tccggtaaga tcgttttcga aatggaagac gtttgctatc
     4981 aggttgacgg taagcaactg gtgaaagatt tttctgccca ggttctacgt ggcgacaaaa
     5041 ttgccctgat tggtccgaat gggtgcggca aaaccacgct gctgaaacta atgctcggtc
     5101 agcttcaagc agacagcggg cgtattcacg ttggcaccaa actggaagtg gcttatttcg
     5161 atcagcaccg cgcggaactg gatcccgata aaacggtgat ggataacctt gccgaaggta
     5221 agcaagaggt gatggttaac ggcaagccac gccacgtatt gggctatttg caggacttcc
     5281 tgttccatcc aaaacgggcg atgacgccgg tacgtgcgct ttctggcggt gagcgtaacc
     5341 gcttgttgtt ggcgcgtttg ttcctcaaac caagcaactt attgattctt gacgaaccga
     5401 ccaacgatct tgatgtcgaa acgctggaac tgctggaaga actgatcgac agctatcagg
     5461 gcacggtatt gctggttagc cacgatcgtc agtttgtcga taacaccgtt acagaatgct
     5521 ggatcttcga aggcggcggt aaaattggtc gttatgttgg cggttatcat gatgcccgtg
     5581 gtcagcaaga acagtatgtg gcgctcaaac agcctgcggt gaaaaaaacc gaagaagccg
     5641 ccgtgccaaa agcagaaact gtaaaacgca gcagtagcaa actaagctat aaattgcagc
     5701 gcgaactgga gcagctaccg caattgctcg aagatctgga ggcgaagctg gaagccctac
     5761 agacgcaggt ggcggatgct tccttcttca gtcagccgca tgagcagaca caaaaagtgc
     5821 tcgccgatat ggctgctgca gagcaggagc tggagcaagc ctttgaacgc tgggagtatc
     5881 ttgaagcgtt aaaaaatggt ggctgatcgc aaaatagtcg atttaaacag agacagctga
     5941 caacggtaaa attgcctgat gaactgggtt tatcaggcat gaaaaccaac actttgttag
     6001 tcatctgtaa aatagcgcat cattaaggag taccaatgtg cgaacatcat catgccgcga
     6061 agcacatcct gtgctcgcag tgtgacatgc tggtggcgtt accgcgcctt gagcatggtc
     6121 agaaagcggc atgtccccgg tgtggcacaa cgttaaccgt ggcgtgggat gcccctcggc
     6181 agcgtccgac cgcctatgcg ttggctgcac tgttcatgct gttgctgtcc aacttgtttc
     6241 cttttgtgaa tatgaacgtt gcaggagtta ccagtgaaat aacattactg gaaattcccg
     6301 gcgtgctttt ttctgaggac tacgccagcc tcggcacctt tttcctgttg tttgtgcaac
     6361 tggttcccgc gttttgtctg ataaccattc tgttactggt gaatcgcgcg gaattaccgg
     6421 tccgtttaaa agagcaactg gcacgggtgc tttttcaact caaaacctgg ggaatggcgg
     6481 agattttcct cgctggtgtg ctggtcagtt tcgttaaact gatggcttac ggcagcattg
     6541 gggtaggcag cagctttctc ccctggtgtt tattttgtgt cctgcaactg cgcgcttttc
     6601 agtgcgttga tcgtcgctgg ttatgggacg acatcgcccc gatgccagaa ctgcgccagc
     6661 cgctaaaacc aggcgtcacg gggatacgtc aggggctgcg ttcgtgctcc tgttgtacgg
     6721 caatccttcc tgctgatgaa cccgtgtgcc cgcgttgtag taccaaaggg tacgttcggc
     6781 gtagaaacag cctgcagtgg acactcgcgc tgcttgtaac gtccatcatg ctgtatcttc
     6841 cggctaatat tttgcccatc atggtgacgg atttattagg ctcgaagatg ccgtcgacga
     6901 ttctcgctgg ggtcattctg ttatggagcg aaggctctta tcccgtcgct gcggtgatct
     6961 ttctggccag tattatggtg ccaacgttaa agatgatcgc catcgcgtgg ctgtgttggg
     7021 atgccaaagg gcatggcaag cgcgacagtg aaagaatgca tttgatttat gaagttgttg
     7081 agtttgtagg ccgctggtcg atgattgacg ttttcgttat cgcggtgctc tcggcgctgg
     7141 tgcgtatggg aggtttaatg agtatttatc cggcaatggg tgcattaatg tttgctttag
     7201 tcgtcataat gacaatgttt tctgctatga cgtttgaccc gcgtttgtcg tgggatcgtc
     7261 aacctgaatc agagcatgag gagtcctgac agtatggaat ctaataatgg ggaagccaaa
     7321 atccagaaag tgaagaactg gtctcccgtg tggatatttc ctatcgtcac ggcgctcatt
     7381 ggggcctggg ttctttttta tcattacagc catcagggac cggaagtgac cctgatcacc
     7441 gcgaatgcgg aaggaattga aggtggcaaa accaccatta aaagccgtag cgttgacgtc
     7501 ggcgtggttg aaagcgccac actggctgat gatttgacgc acgttgaaat caaagcgcgg
     7561 ctgaattccg gtatggaaaa attgctgcat aaagacaccg tcttttgggt ggtgaaaccg
     7621 cagattggtc gcgaagggat tagcggcctg ggaacgctgc tgtctggagt ttatatcgaa
     7681 ctgcagccag gcgcgaaagg cagcaaaatg gataaatacg atttgctgga ctcgccaccg
     7741 ttggccccgc ctgatgcgaa aggtatccgt gtgattctcg atagcaaaaa agccgggcag
     7801 ctctcgccag gagatccggt gcttttccgt ggctatcggg taggttcggt tgaaaccagc
     7861 accttcgata cgcaaaaacg caatatcagt tatcaactgt tcatcaatgc accttatgac
     7921 cgactggtga ccagcaatgt tcgcttctgg aaagatagtg gcattgcggt tgatctgacg
     7981 tcagcgggga tgcgtgtgga gatgggctca ttgacaacgc tgcttagtgg cggtgttagc
     8041 tttgatgtgc cggaaggtct ggatttaggg cagccagtgg caccgaaaac agctttcgtt
     8101 ttgtatgatg atcagaagag cattcaggat tcgttgtaca ccgatcacat tgattatctg
     8161 atgttcttta aagattcggt acgcggtctg caaccgggag ctccggtaga attccggggt
     8221 attcgcctgg gtaccgtaag caaagtgcca ttctttgcgc cgaatatgcg tcagacattt
     8281 aacgatgatt accgtattcc ggtactgatt cgtatcgagc cagagcggct gaaaatgcag
     8341 cttggcgaaa atgcggatgt tgttgagcac cttggcgaat tgttgaaacg tggtttacgc
     8401 ggatcgctga aaaccggaaa cctggtcact ggtgcactgt atgttgatct cgatttctat
     8461 ccaaatacgc ctgcaataac cggtattcgt gaatttaatg gttatcagat tatcccgact
     8521 gttagcggcg gcctggcgca aatccagcaa cgactgatgg aagcgttgga taagatcaac
     8581 aaactgccat tgaatccgat gattgaacag gcaaccagta cgctttctga aagtcagcgc
     8641 acaatgaaaa acctgcaaac gacgctggat agcatgaaca agatcctcgc tagccagtcg
     8701 atgcagcagt tgccgacgga tatgcagtca acgttgcgtg aattgaatcg cagcatgcag
     8761 ggcttccagc ctggctccgc agcctacaac aagatggtgg cggatatgca gcgccttgat
     8821 caggtgttgc gagaactgca accggtgctg aaaacgctca atgagaagag taacgcgctg
     8881 gtatttgaag cgaaggacaa aaaagatcca gagccgaaga gggcgaaaca atgaaaaagt
     8941 ggctagtgac gattgcagca ctgtggctgg ccggatgcag ctccggcgaa attaataaaa
     9001 actattacca gttacctgtg gtgcagagcg gtacacaaag taccgccagc cagggcaatc
     9061 gtctgttatg ggtagagcag gtcactgttc ctgactatct ggcggggaat ggtgtggttt
     9121 atcaaaccag tgatgtgaag tatgtgattg ccaacaacaa cttgtgggcc agcccgttgg
     9181 atcaacagtt gcgcaacacc ctggttgcca acctgagtac gcaactgccc ggctgggtgg
     9241 ttgcctccca gcctctggga agcgcccagg acacgctcaa tgttaccgta acggagttta
     9301 acggtcgcta tgatggcaag gtcattgtca gtggtgagtg gctgttgaac caccagggac
     9361 aactgatcaa acgtccgttc cgtctggaag gagtgcaaac tcaggatggt tacgatgaga
     9421 tggttaaagt gctggccggt gtctggagtc aggaagccgc ttctattgca caagagataa
     9481 agcgtctacc ttaattataa agatttgtaa atataaccgt ctccggtatg ttgcctgagg
     9541 cggttttttt gtttctaacg tgcggaaaaa tttgttcctc ttcacatttt ttgtacaacc
     9601 gacatgcccg tgtagctcac aaatatgaca gtggcgtgaa ttttgcgcat tgacggcagt
     9661 tatgattcgc ggtattgctt aactgtgatt gcacatttag taatcactgt tttcttttcc
     9721 accagaaacc agtatgaggg aaacgaggca tgaagagaca aaaacgagat cgcctggaac
     9781 gggcacatca acgtggttat caggccggca tcgccggacg ctcaaaagaa atgtgtccct
     9841 atcagacgct gaatcaaagg tcacaatggc tgggaggctg gcgagaagcc atggcggaca
     9901 gggtagtaat ggcctgattc tgtctcttta aaaagaaacc tccgcattgc ggaggtttcg
     9961 ccttttgata ctctgtctga ttataatcag aaggcagacg tatcctggaa cagaccgact
    10021 ttcaggtcgc tggcggtata gataagacga ccatcaacca gcacttcgcc atccgccagg
    10081 cccataatca gacgacggtt aacaatgcgt ttaaagtgaa tacggtaggt cacttttttc
    10141 gctgtcggca gtacctgacc agtgaatttc acttcgccaa cgcccagcgc gcggccttta
    10201 ccttcgccgc ccagccagcc gaggtagaac cctaccagct gccacattgc gtccaggccc
    10261 aggcatcccg gcataaccgg atcgccaata aagtggcatc cgaagaacca cagatccgga
    10321 ttgatatcca gttctgcttc aacataccct ttgtcgaagt taccacccgt ttcggtcatt
    10381 ttgaccacac ggtccatcat cagcatgttc ggtgctggca attgcgggcc tttagcgcca
    10441 aacagttcac cgcgaccaga ggcaagaagg tcttcttttg tataggattc gcgtttatct
    10501 accatgttct ctgtaagcct tattttattg aagcacgcag gatagctaac acgtgtacgc
    10561 tgaacaagtc cgatcagttc ggaataaacc agttcagcca acgtaatggc catggaaaac
    10621 ggtgacgtcc ttcctgttgc gatgcttgcg cgatacgttc ctggatggtt tgcatcagcg
    10681 tcgtttggcc ttcgccatcc cacaccagat ttaataataa cggcagtgcg tcagtcacat
    10741 cgtctactgc ccagatggtg aatttgcctt cttctaccgc tttcaccagt tcactgtgaa
    10801 gacttaaatg gcgaacgtta gctgtgggga taatgacacc ttgtttcccg gttaactcac
    10861 gttgctggca aatagcaaag aagccttcga ttttctcatt taaaccaccg accggctggg
    10921 cgcgaccgaa ctgatcgact gaacctgtga tagcgatact ctgattcacc ggcacatcgg
    10981 cgagggcgct tatcagggcg cagagttcag ccatcgaggc actatctcca tcaacttcac
    11041 tgtatgactg ctcaaatgtc agcgatgctg agaaggggat ctgttgctca agctgtagtt
    11101 ccgacatcag gaacgcttgc atgatcatca tccctttcgc atggatattg ccgccaagct
    11161 ccgctttgcg ttcgatgtcg gtgaattcac catcgccaat atgcacaacg cagctaatgc
    11221 gagaaggttc gccaaaagcg cgtggatgac ccggaaattc aatgaccgaa agggcgttaa
    11281 tttgcccgat gcgttcgcct tcggtttcaa tcaggatttg ctcctgaagg atctcatcct
    11341 gcatacgttc agcgaggaaa ccttcgcgcc attcacgctg ctgcagcatt aagtttagct
    11401 gctcgccgga gaaggtgtcg ccatcacaca gggaggcgac ctctttacac tggcggagga
    11461 tccactgcgg gctaagcgga agtgtttctt gttcaccggt gtagcgtgct gcttcgcgga
    11521 taagtaccgg ccaggcatcc gctcccggtg caggtaagtg attatgtctg gcggtaaatg
    11581 tcacccagcg acaccactgg gttactgact ctgcatcgac aatctgcaga gtatcttcaa
    11641 attcgctata aatagcctgc tctgaaagct ctggctccat ctcctggaaa tcagccaatg
    11701 attcgcgttc gcctaccaga atgactttca gcttcaatgg catcgaaggc acagagacgg
    11761 ggagagggcg cgactcatca aacgcaaccc agtcaaaacg ctcgcggtta acgatatttt
    11821 tcagccgcat ccacagcaga ggttgcgcca gcagtgtacg caaagagatg atgagaatac
    11881 cgccatttgc ctgatgcacc agaccaggct gcagggtaat gtcgccatta aactggcgca
    11941 ggcagccaaa gagttgctcc gcttctaccc agtcggcagc gacaacttgc gttaaagtcg
    12001 caaaattatc atctgcactc actgcgtgac gtaagcggat ggagtggccg gaaacctcat
    12061 agtgaccgcc caccagttgg cctgcatcgc tttgtagcgt acgcgcggca ttggcaataa
    12121 gattcagata ctcagactct tccggggcct tcgccagcat aaaagaggag aatgctcgcg
    12181 tatgcagcaa ctgctccagc gcaaattgca gccgcggttg agtatcactg aataaaggat
    12241 cgttttcgtc aatcaaatgt ggctgagcaa atatttcctg atagctatcg gtatcaggaa
    12301 ccaggtcacg ccatgcaagt ttcgtaatgg tcaaagttga tgttttttag tctgttgtca
    12361 aagccgcgat tataccgtaa ccggcactac agcacacgta gaaagcaccg acaatactcc
    12421 tggcatgggc gttaaagctc acaggatgga gattcttttc ttcactggcc taaaaagctg
    12481 atattctgta aagagttaca cggtaacatt gagatcgcta tgaaatatca acaacttgaa
    12541 aatcttgaaa gcggttggaa atggaagtat ctggtgaaga agcatcgcga aggggagtta
    12601 atcacccgtt acatagaagc cagtgccgcc caggaagccg tcgatgtgtt gctctcgctg
    12661 gaaaatgaac ccgtgctggt aaatggctgg attgacaagc atatgaatcc ggaactggtc
    12721 aatcgcatga agcaaaccat tcgggccaga cggaagcgcc attttaatgc ggaacaccag
    12781 catacgcgca aaaaatcgat cgatctggaa tttattgtct ggcaacgtct ggctggtctt
    12841 gcgcagcgtc gcggtaaaac gctttctgaa acgattgttc agctgattga agatgcggaa
    12901 aacaaagaga aatacgcgaa taaaatgtct tctctgaagc aggatctgca ggcattgctg
    12961 ggtaaggaat aactgacgaa agtcagttca atttactaaa ggcaaaaaaa accccgcagc
    13021 agcggggttt ttctaccaga cgagaactta agcttgcggc tgagttacaa cgtctttgat
    13081 acctttaact tcgatctcta cgcgacgatc cggagccagg cagtcgatca gtgcagcacg
    13141 ctgtttcacg ttgtcacagg tgttgccagt aaccgggttg gattcgccca taccacgtgc
    13201 ggagatcttg tctgccggga tacctttgga gatcaggtaa tcaacaacag actgagcacg
    13261 gcgctcggac agaccctggt tgtaagcgtc agaaccgatg cggtcggtgt aacccagaac
    13321 aactacggaa ccgtctttcg gatccaggtt gctcagctgg ctgtacagct gatccagagc
    13381 agcctgacct tccggtttca gggttgcttt gttgaagttg aacagaacgt cagacttcag
    13441 agtgaagtgc ttggtctgta cttccggtgc cggagctgga gccggagcaa ctactggagc
    13501 tgcttcgccc tgaccgaaac ggtaggaaac acccaggctc agcatgccgt tgtccggacg
    13561 agtgccgatg gtgtgtgcgt caccgatgtt gttggtccac tggtattcca gacgggtagc
    13621 gatttcagga gtgatcgcgt actcaacacc acctgcgaaa accggagaaa cgccagtgtc
    13681 gtggtcttta aaggatgcgc caccaggtac gttagccttg gtgtctgcac gccataccat
    13741 accacccaga cgagtgtata cgtccagatc gtcagtgatt gggtaaccca gtttagcagt
    13801 cagttgaacg ccctgagctt tgtatgcacc gttgatgttg tcgcctttgt acggcatacg
    13861 acctaaccag tcgtaaccca tttcaaaacc aacatacggg ttaacctggt aaccaccaaa
    13921 agcacctgca cccagttggt tttcgtgggt cggaccattg ttaggaataa aaccagtgtc
    13981 atggtactgg gaccagccca gtttagcacc agtgtaccag gtgttatctt tcggagcggc
    14041 ctgcgctacg gtagcgaaac cagccagtgc cactgcaatc gcgatagctg tctttttcat
    14101 tttttgcgcc tcgttatcat ccaaaatacg ccatgaatat ctccaacgag ataacacggt
    14161 taaatccttc accgggggat ctgctcaata ttaactctac cgatatcttc ggcttatgcc
    14221 gagcacccct ggcgatgtaa agtctacaac gtagttgaaa acttacaagt gtgaactccg
    14281 tcaggcatat gaaaaaaaag tcttgtataa ggtatgttta atcttttttg tcagcgacaa
    14341 tttacagaag aaaatcgcgg aaaccgcttc agacaagcct ccgcaaggaa aattagtcac
    14401 gactgaaagc attggctggg cgacaaaaaa agttccagga ttaatcctaa atttacttaa
    14461 tgatacaaat tagagtgaat ttttagcccg gaaagttgtc tcgtggcgtg agaggatgcg
    14521 cttaccggac gcataataaa ccccatagcg ttaccttcat ttgccgcatc aacaagttca
    14581 gcatgctctt cttcagtcaa atcatctgcc aaccaaccga tcaccacact gtaattgccc
    14641 gtgcgtaaag cgcgaaccat tgactccaca gtgtggcaag gggagagctg gctaatctgc
    14701 attactttcg ttaagggtag cccagatgcc tgaacccatt cccgactcag tttttgttgc
    14761 ggtgttaacc agagttgcca gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt
    14821 agaagttgcg tcatcatggg ctgatcttcg cgatagacaa cttcactgat aagcccggct
    14881 gtagtgtttt ccgtagagac acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa
    14941 cgatgtgcat agcctgaagt gtacataatc aatccagccc ctgtgagtta ctgtatggat
    15001 atacagtaca tccagtgaca acaaagatca accctatttt cggaaagagc ctcgcaaatt
    15061 ttgtcgttgg tgacgggaaa acataaatta atcttgcccc ttaagaataa gttgcctatt
    15121 ttcgtagtta acggatccgt taatgtgaat cattctttta tgttatgatt ttaaaaggaa
    15181 ttttatgaaa agcctctcct ataagcggat ctataaatca caagaatacc tggcaacgtt
    15241 gggcacaatt gaataccgat cattgtttgg cagttacagc ctgaccgttg acgacacggt
    15301 gtttgcgatg gtttctgatg gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta
    15361 ctgtgtaaaa catccgcctg tctggctgac atataaaaag tgtggccgat ccgttaccct
    15421 caattactat cgggttgatg aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc
    15481 gaaatattct ctcgatgcag cgctgaaaga gaaaagcacg cgcaataccc gggaaagact
    15541 gaaagatttg cccaatatgt cttttcatct ggaagcgatt ctcggggagg tggggattaa
    15601 ggatgtacgg gcgttacgta tacttggggc aaaaatgtgt tggttgcgac tgcggcagca
    15661 aaacagtctg gtgacagaaa agattctgtt tatgcttgaa ggtgccatta tcggcattca
    15721 tgaagctgcg ctcccggtgg cacgccgcca ggagcttgca gaatgggctg actctcttac
    15781 gccgaaacag gagtttcctg cggaacttga gtaatctggc gttgcagacg accaatctca
    15841 ggcagtaatg caatcaataa tccaacttgt tgtacgacca gaggttcttt gctgtctgcc
    15901 cgtggttcaa gttgctgcat ccgctgtttc aggctagcta atgcctcatt gacgcgttct
    15961 tcatcagcag gttgatgatg taacgcgtca tcaacatagc aaactgcgtc atcaagaaac
    16021 gccagaattt caggattagt taactgctcc cggtgagcac cgagggctga gatatagctg
    16081 gtaaacgtat ggttaaggca cagcaaccga aacgcggctt cgcgaatttg cggggtaacg
    16141 tttggctcgc tggacatatt tgataccacc gacgccagct cagcatcacg gttgtgtgca
    16201 tcgcggcggg caatacgata cgccagacgg ttatcacgcc cctgatggta ttgctccagt
    16261 atggcatcga gataccgaca attggcctct gtggcgcgtt cgagcatgcg cggcagattg
    16321 cgaaactgcc agtcaggcca gatgtagctc actgccgccc acgcaatggc acaaccaatc
    16381 agcgtatcga ttacgcgagg taacgctact tcaaaacctt cacccagtaa gttaaaacac
    16441 agtagcacca aaagtgtgat gaacatcgtt gcatgagcgt attgcacgtt acggaaggca
    16501 aaaaagagca cgccggtaat aaccagcagc accagctgcc cttccagtga tggcacaaac
    16561 cacagcacag gaatgccaat ggcgatacct accagcgtac caataatcct taacttcagg
    16621 cggtggcgcg tggcgttata gtttggctgg cagacaaaca aacttgtcag caagatccaa
    16681 tacccgtgat gcattccggt tatttgaatg atggcgtagc cgaagcacaa caccagcgac
    16741 attcttaccg catgacggaa gagggcggat tccggcgtga agtgacggct aagacgcagc
    16801 cagatatcac tcaacccgtg cgggctgtca tcagcgagct cattttcgtc attattatgg
    16861 ggtagtgcct gggcctgttc tgattcaatt gttgccagtt gggcatcaat ggcgcgtaaa
    16921 ttgttcagca aaaatcccag tgttttgagt aaatcggcgg gtgcgccgtt atcgcgcatc
    16981 cgctccagcg cagcatcaat atgcgtaaaa gcgcgctcaa aatgcggatc atgttgataa
    17041 ggctgacgca gcaaaataca gcgtgacagt tgctggcacg cctggccctg catcgacatc
    17101 agccgctgaa aacggaacag cacgtcgctg tggcgaaaat gttcacgcaa tgtttgatac
    17161 tgaatatgag aagagctggc acgctcgtga atatcctgcg cgacaaagta ataatgcagc
    17221 gtgcgacgcg ttccccgttg accacgatcg ccacgtaagc gggtcagcag cgagagtttc
    17281 gtctgattca atgtcgccat cagttgtccg ttggcgagag ccaaatcgta cagcggtgcc
    17341 tggctttcat cttcaatatc aggatcaaac atgcgcgact tgagctcaag ataacgcgcc
    17401 agttgttcat agcaacgcgc caggttgtcc tgcagcgggc ggaccgggaa cagcagatga
    17461 ccaataagtg tcaggacgtt gtaccagacg gcaccggcca gcagatacat cggctgctga
    17521 taccagtgct catacagtga tgttcccaac atagtgtaaa tggcgatcag caatgcaccg
    17581 aaggcaattg ttgcatagcg ttgacccaga ccgccgagca aaatgaagcc gctggtagag
    17641 agcgttaagc caatcgcaaa tagccagggc cagggaaaca gcaattctac tgaggccgag
    17701 gcgataaaaa agcagaacag cgtaatgatg aggttacgca aacgtcccgc cagtcggtca
    17761 tcgagatcgg tcagcgccgc tgccaccatc cccagcgtta gcggaatcgt cagttttaca
    17821 tcacccagcc accacggaaa cgctgtggtt ccacaaagcg caataaaaat acgcgcgtaa
    17881 tacagccagg cgctgttcca ggtatagcgt ttgagcaaag gacttagcat aaaggccata
    17941 ttagtggatt attcaaaacg acgacgtgca ttggcttcac gcgcagcttg cgctgtttct
    18001 accgatacca cgcgacgacc aaccggccat agtgcaatag cggcaatttt aaagttcgca
    18061 atgccaacag gaatgccaat gattgaaata cattgtgcga tgcccgttgc aatgtgcatc
    18121 aggcataacc accagccaaa gaaaatcagc cagaaaatat tcaataccgt accgccagta
    18181 ttcagcagca catttttgcc tgccgggttc agttcatcga catgaatagc ttcattgcca
    18241 taaggcacca gagacagttt agtgatctcc cagcaggatc gtgtcagcgg taaggtaaaa
    18301 atcagcacaa tactgaccag agtcgccaac agccagccca gagtggtggc aaatccgcca
    18361 agcacaaagt tcagaatgtt caaaacggta cgcataaaac ctcgctttac tgtggttttc
    18421 agtaatgggc ggcaattgta acgttttttt gggctggagc acgttttctc tgacggttac
    18481 actgataaga aataatttcg tgtggatcta cagagtcatg gaactgaaag cgacaacgct
    18541 tggaaaacgt ctggcacagc acccttacga tcgggcggtg atcctcaatg ccgggattaa
    18601 agtctccggc gatcgccacg aataccttat tcctttcaat caattactgg cgattcactg
    18661 taagcgcggt ctggtatggg gcgagctgga atttgtactg ccggacgaaa aagtggtgcg
    18721 tctgcacggc accgaatggg gcgagacgca gcgtttttac catcatcttg atgctcactg
    18781 gcggcggtgg agtggcgaga tgagcgaaat tgcgtctggt gttttacgcc agcaactgga
    18841 tttgattgcc acgcgcactg gagaaaataa atggctgacg cgtgagcaaa cctctggcgt
    18901 tcagcaacaa atccgccagg ctttgtcggc gttgccgttg ccggttaacc gactggaaga
    18961 attcgataac tgccgtgagg cgtggcgtaa atgtcaggcc tggttgaaag atattgaaag
    19021 cgctcggttg cagcataacc aggcgtatac cgaagccatg cttaccgagt atgcggattt
    19081 tttccgccag gtcgagtctt caccgctgaa tccggcgcag gcccgggcag tcgttaatgg
    19141 cgagcattct ctgttagtgc tggcaggtgc aggaagcgga aaaacgtcgg tgctggtggc
    19201 ccgtgcaggc tggttgctgg cgcgtggtga agcgtcccct gagcaaattt tattgctggc
    19261 gtttggtcgc aaagccgctg aagagatgga cgagcggatt cgcgaacggc tacataccga
    19321 agacattacc gcacgcacgt ttcatgcgct ggcgctgcat attattcagc agggcagcaa
    19381 aaaagttccg atagtcagca aactggaaaa tgataccgct gcccgtcatg aactctttat
    19441 tgctgagtgg cgcaagcaat gcagcgaaaa gaaagcgcag gcgaagggct ggcggcaatg
    19501 gctgacggaa gaaatgcagt ggtcagtgcc agaaggtaac ttctgggatg atgaaaaatt
    19561 acagcgtcgc cttgcctctc gcctcgatcg ctgggtaagt ctgatgcgga tgcacggtgg
    19621 tgcacaggca gaaatgattg ccagtgcacc cgaagagatt cgcgatctgt tcagtaaacg
    19681 tatcaagttg atggccccgt tattaaaagc ctggaaaggt gcgctgaagg cagaaaacgc
    19741 tgtcgatttt tcgggcctta ttcatcaggc gattgtgatt ctggagaaag gtcgctttat
    19801 cagcccgtgg aagcatattc tggttgatga atttcaggat atctcgccgc agcgggcagc
    19861 gttgttagcg gcattacgca agcaaaacag tcagacgacg ttgttcgctg ttggtgatga
    19921 ctggcaggcg atttaccgat tcagcggtgc gcaaatgtcg ctcaccaccg ctttccatga
    19981 aaactttggt gaaggcgaac gttgtgattt agacacgact taccgtttta acagtcgtat
    20041 cggtaaggtg gcaaaccggt ttattcagca gaacccaggc cagctgaaaa agccgctaaa
    20101 cagcttaacc aatggagaca aaaaagccgt cacgttattg gatgagagtc aacttgacgc
    20161 tttgctggat aagctctctg gttatgccaa accggaagag cgcattctga tcctggcgcg
    20221 ttaccatcac atgaggcctg ccagcctgga aaaagcggca acacgctggc cgaagttgca
    20281 aatcgacttt atgaccattc atgccagcaa agggcaacag gcggattacg tcatcatcgt
    20341 tggcttgcag gagggaagtg atggttttcc ggctgcggcg cgggaatcga ttatggaaga
    20401 ggcgctactg ccaccggttg aggatttccc ggacgcggaa gaacggcggt taatgtacgt
    20461 ggcgctgacc cgggcacgcc atcgggtatg ggcactgttt aacaaagaga atccctctcc
    20521 ctttgtggaa atactgaaaa atctggatgt gccggtggcg agaaaaccgt aagaaacagg
    20581 tggcgtttgc cacctgtgca atattacttc agacggtccg cgagataacg ctgataatcg
    20641 gggatcagaa tatcgaccgc gtcgttgaaa tgcggcgact ggattatgaa gtctgccgtt
    20701 gccacgttgg tggcgaccgg aatgttccat accgtcgcca gacgcagcaa ggctttcacg
    20761 tcaggatcgt gcggcacggc atttagtgga tcccagaaga aaatcaatac atcaattttc
    20821 ccttctgaga tcaatgcgcc aacctgctgg tcacccccca ttgggccact caacatcgcg
    20881 ttgacgttca tgccggtcgc gcgggaaatt aagttaccgg tagtgcctgt tgcatacagt
    20941 acgtgttgtt ccagtaacgg ttgatgccgt tccacccagc tcatcagcat ttgtttgcag
    21001 tgatcgtgtg ccaccagcgc aatatgtttc cgcgcaggta aagtgcgagt cgtcagttcc
    21061 ataatgtaca tccgtagtta actttcctac agattactgt aagcacttat cgctgcaaga
    21121 taaagaccga aaaagcctgc gcacaggcac aaaaatctca ggaagatggt tgtttttccg
    21181 cccattgcag gaaagtattt cgcgtttgtg ggtcagccag tttaaaccaa tacttcagcc
    21241 gttgttctgt gagcacctga gactgcggtg gaatagcatc cagctcggaa acacccgaaa
    21301 gcagcgtact gtcatctgcc atcatcgtgg caaattgcgg tagtgaagcg cgtttcgcgg
    21361 atttgttata gcgttcaacc tcgacctcat aatcaatcgt ttttgccgtt gaggtaatgg
    21421 cgagaatatc cagctttacc ggaatcggcg tcgcatcgcc atccaacaat tcaaggcgcg
    21481 gcgcggcatc aaaatggtta gcttcccgct cattctccag gcgaggcagg cgaaaattga
    21541 cctggttgat gagctgggta ttaaaactga ccaccagcgg tggggagata tacagccgtt
    21601 cttcactatt ggaaagatga atcgtcttct caacgcgaaa cactaactga tgcggtccat
    21661 tatccagttc aatgctgtcc gcaccgcgca gcagagagct ggaaaccttc ttgccgtcga
    21721 gtaccagaag atcgacatcg gttgaaagcc gcagcgtggt ggcaaaaacg gataccggca
    21781 aacataatgc aatcaaggtg gtcacgatgc cggttttcat aggtagctcc tgtcaaaaga
    21841 ccgcccgaat aataatgtgt caaaattttt ctttaatcat acttactaac atatagacat
    21901 atttcccgct tttgctctca atcattcgta ttagctgcat ggttggcatg tcggctttgt
    21961 cgtacacttt gttaaacagt ctggaggaaa taatgaaaga aaccgatatt gcaggtattt
    22021 taacgtccac tcacaccatt gcgctggtgg gggcaagcga caaacccgat cgtcccagct
    22081 atcgcgtgat gaaatatcta ctcgaccagg ggtatcacgt cattccggtt tcgccaaaag
    22141 ttgccggcaa aacgctgctg ggccagcagg ggtatggcac gctggcagac gttccggaaa
    22201 aagtcgatat ggtcgatgtt ttccgtaatt cagaagcggc gtggggcgtg gcgcaggaag
    22261 ctatcgccat tggcgcgaaa accctctgga tgcaactggg ggtgattaat gagcaagcgg
    22321 cagtactggc acgggatgcc gggttaaatg tggtgatgga tcgctgcccg gctattgaga
    22381 tccctcgcct gggcctggcc aaataaaaaa tccccggaag gcaaaaacct tccggggatt
    22441 cgttcaggga tcagttacgc agacgcgggg cctggagttg tttgcggatg gtctgcgcca
    22501 gctcatccat ggatggctgt tcaggatgtt catcctgcaa ttcgctactt agctgggctt
    22561 cagccagata ggtatgaacc ggtaggccgt tatcgtcctc catcaccacg tgataccacg
    22621 gagcggcgcg aagctcgtca ttcaccgcca gctcatcagg cgacggttca gagagcgaat
    22681 aaaccgggtc gatatccacg accactccga gataacctaa cagggaatgg cggacctgct
    22741 ggccgatacc gaatttgctg gcaatcatag tcacctcccg ggaaatcttt acctttgata
    22801 taagggtaaa attccacatt tcaagttaca tgacgcgaca ggcaaaccct ttcagatata
    22861 gcccttccgg ataggtagcg atcaccggat gatcggctgc ctgacggaac tgctctataa
    22921 attgtacatc acggccggca tcaattgcgg catccgcgat gattttctga aataaatcgc
    22981 tggtcatcag accggaacag gagaaagtca ggagaatacc gccttcattc agcaactgaa
    23041 tcgccagcat gttgatatct ttatagccac gacacgcgcc catcaactgg cttttattct
    23101 caacaaactt cggcgggtcc atcacgataa cgtcaaattt ttcaccgcga tcgcgataag
    23161 tacgcagcaa tttaaagaca tcatcacgga caaactcagc cttgctcaga tccagtttgt
    23221 tcagctcaac gttctgccgt gcaatatcca gcgcttcctg ggaggtatca acgctgacaa
    23281 cctggctgca accgcccatc agtgccgata cggcgaaacc accggtatag gagaaacagt
    23341 tcagcacacg tttattttca acgtagcggc gggtagccag gcggctgtct cgctggtcca
    23401 ggtagtagcc cgttttgtgt ccgtgctgaa tatccaccag cagtttcatg ccgtgttctt
    23461 caatcggcag cagggcaggt ggcaactcgc cggtgacgag gccctgggtc agctccatcc
    23521 cttctttttt acgtaccgca acatcgctgc gatcgtaaat cgcacattcc gggtacagcg
    23581 tttgcagggc actaattaat gccgcgcgct gatattctgc gccagcactc agcagttgca
    23641 gcaccagaaa attaccgaaa cgatcgatag taatacccgg caggccatca gattctccgg
    23701 cgattaaacg atagctgtcg aggccatctt tttgcgccag ccagtcacgc catttttgtg
    23761 cttgttgcaa acggcgggaa aaaaaagcaa tgtcgataga ctcagacggg tcaaacgtcc
    23821 agacgcgcgc ccggatttgc gaagctggcg aataagcgcc gcgtgctaac cattttccct
    23881 gatgatcaac aatatcgatg gtttcaccga ggctggcttt accttccatg cgggcaacgg
    23941 ccccggaaaa gacccacgga tggcgacgaa gtaatgattt ttcgcgccct ttggctaaca
    24001 ctaaacgtac actcataatt tacttttctg tcgatgcaaa gaaatgggcg tcattgtccg
    24061 gagttttcca tggatttgca acgcgtccat taaggataag gagagcagca atgtcgaaag
    24121 tctgcataat tgcctgggtt tacgggcgag ttcagggcgt aggatttcgc tacaccacac
    24181 agtatgaagc gaaaagactg ggtttaaccg ggtacgccaa aaatcttgac gatggcagcg
    24241 tggaagtggt tgcctgcggt gaagaagggc aggtggaaaa attaatgcag tggttaaaga
    24301 gtggcggccc gcgttctgcg cgtgtggaac gggtgcttag cgagccgcat catccctcgg
    24361 gggaattagc cgattttcgt atccgttaaa tacattttac cggttttggc aggccagcaa
    24421 ttttggtggc ttgcttagcc ggacctttcg gaaacagtcg atataaatag cggctgttac
    24481 ctttttcttc gccaaattta ttcgccatcg cttttaccag catacgaatc gccggagaag
    24541 tattgaattc cagatagaaa tcacgcacaa aacgcaccac ttcccagtgt tctggcgaca
    24601 gcgaaatccc ttcgttctct gcaatcacca cagccagcgg ctcactccac tggctgcttt
    24661 ctttgagata gccttcggta tccgtttcta tctctttacc ttcgaagatc agcataatta
    24721 ttactacctt aatcagactg gcggcagtgt aacaaaaaac aaagccccgc ataaagcgag
    24781 gctatgaaag tgttagcggg tgagattaat cgcggctagc gaagcccaga atgctcagca
    24841 ggctgacgaa gatgttgtac agcgaaacat acaggctaac cgtggcacga atatagttcg
    24901 tctcaccgcc atgaatgatg ttgctggttt caaacaagat agcgccagag gagatcagaa
    24961 tgaagaccgc gctgatcgcc agatgcagag caggcagttg caggaagata ttagcaacca
    25021 taccaatcag caccaccaca atacccgcca tcagcatacc gccgaggaac gacatatctt
    25081 tgcgggtggt cagcacatat gcagagcagc agaagaacac taacgccgtt ccgcccagtg
    25141 ccatagcgat tacgtcaccc attccggcag acagataggt gttcagaata ggtccgagga
    25201 tataacccag aaaaccggta aaggcgaatg cggagataat cccggtcggc ttattcgccg
    25261 ttttataggt caggaacatc aaaccataca tacccaccag cgtcagaatc agacccggag
    25321 atggcagcat cagcaccgta ctggcagttg cggtaatcgc cgaaaaggcc agcgtcaggc
    25381 tcagcagaaa ataggtatta cgcagcacct tatgggtgct aagcagtgat gtacggtcat
    25441 gtgaagaact aacaatacga tccattgagt cactctctat gacagatgta attaattaag
    25501 cagcataatg ataatgcgta agggcaccca gaagttttac ccatctttac gcatttgatc
    25561 tggaacaggt ttaacagcgg attatcaggt cattaagcaa atataacgcc ctgagaattt
    25621 cgacaggcaa aagaaaaagg ggttagcatt tagctaaccc cttatcttat ttggcggaag
    25681 cgcagagatt cgaactctgg aaccctttcg ggtcgccggt tttcaagacc ggtgccttca
    25741 accgctcggc cacacttccg gaatgacgcg cactataaac atcccgatgc ggcgtgtaaa
    25801 cccctaattt gtttgtttgc ctgaaaaaca gccaaaagtg cattgatagc gtgaaataac
    25861 agcagattga tcatttcatc accatgaatt ccttctcttt tactcgttta gcaaccggct
    25921 aaacatcccc accgcccggc caaaagaaaa ataggtccat ttttatcgct aaaagataaa
    25981 tccacacagt ttgtattgtt ttgtgcaaaa gtttcactac gctttattaa caatactttc
    26041 tggcgacgtg tgccagtgca gaaggatgag ctttcgtttt cagcatctca cgtgaagcga
    26101 tggtttgcct tgctacaggg acgtcgcttg ccgaccataa gcgcccggtg tcctgccggt
    26161 gttgctagga gaagagacgt gcgatatgaa taacgaggaa acattttacc aggccatgcg
    26221 gcgtcagggc gttacccggc gcagctttct caaatattgt agtctggctg ccacgtcgct
    26281 gggattaggc gcgggaatgg caccaaagat tgcctgggcg ctggagaaca aaccgcgcat
    26341 tccggtggta tggatccacg gtctggaatg cacctgctgt accgaatctt ttatccgctc
    26401 cgctcaccca ctggcgaagg acgtcatcct ttcccttatt tctctcgatt acgacgatac
    26461 tttgatggct gccgccggaa accaggcgga agaagtcttt gaagacatca tcacgcaata
    26521 caatggcaaa tatatcctcg cagtagaagg caatccgccg ctgggcgagc aggggatgtt
    26581 ctgtatcagc agcggtcgac cgtttattga gaaactcaaa cgtgccgctg ccggagccag
    26641 cgcgattatc gcctggggaa cctgcgcgtc ctggggctgc gtgcaggccg cgcggcccaa
    26701 tccgacgcag gcaacgccta tcgacaaagt catcaccgac aaacccatta tcaaagtacc
    26761 tggctgcccg ccgatcccgg atgtgatgag cgccatcatt acttacatgg tgacctttga
    26821 tcgcttgcca gatgtcgaca gaatgggccg tccactgatg ttttatggtc agcgtatcca
    26881 tgataaatgc tatcgccgcg ctcacttcga cgccggagag ttcgtccaga gttgggatga
    26941 tgacgctgcc cgcaaaggtt actgcctgta caaaatgggc tgcaaagggc ctaccaccta
    27001 taacgcctgt tcctccacac gctggaatga tggcgtttct tttccaatcc agtctggtca
    27061 cggctgcctg ggctgtgcgg aaaatggttt ctgggatcgc ggttcgttct acagccgcgt
    27121 ggtcgatatt ccgcaaatgg gtactcattc cactgccgat accgtcggtt taaccgcgct
    27181 tggcgtggtg gcagcggctg ttggtgtgca cgcagtcgcc agcgccgttg accagcgcag
    27241 acgtcataac cagcaaccta cagaaaccga acatcagcca ggcaatgagg ataaacaggc
    27301 atgagcactc agtacgaaac tcagggatac accatcaata atgccggacg ccgcctggtg
    27361 gtcgacccga ttacgcgcat cgaaggccac atgcgctgcg aagtgaatat taacgatcag
    27421 aatgtgatca ccaatgccgt ctcctgcggc accatgtttc gcgggctgga gatcattctg
    27481 caagggcgcg acccgcgcga tgcgtgggcg ttcgttgaac gtatctgcgg cgtctgtact
    27541 ggcgtacacg ccctggcttc ggtttacgcc atcgaagatg ctatcggtat taaagtgccg
    27601 gacaacgcca atatcatccg caacattatg ctggcaacgc tctggtgcca cgatcatctg
    27661 gtgcacttct atcagcttgc cgggatggac tggatcgatg tgttagatgc gctgaaagcc
    27721 gacccgcgga aaacctccga actggcgcaa agtctctcct cttggccgaa atcatcccct
    27781 ggctatttct tcgacgtaca aaaccgcctg aaaaaatttg ttgaaggcgg gcagttgggg
    27841 atcttccgca atggctactg ggggcacccg cagtacaaac tgccgccaga agctaacctg
    27901 atgggctttg cccactatct cgaagctctc gatttccagc gtgaaattgt caaaatccac
    27961 gcggtctttg gcggtaaaaa cccgcatcca aactggattg tcggcgggat gccttgcgcc
    28021 atcaacattg acgaaagcgg cgcggtcggg gcagtcaata tggaacgcct gaacctggtg
    28081 cagtcaatta tcacccgcac ggcggacttc attaacaacg tgatgatccc cgacgcctta
    28141 gccatcggtc agttcaacaa accgtggagc gaaatcggca cgggtctttc tgataaatgt
    28201 gtcctcagct acggggcatt cccggatatt gccaacgact ttggtgagaa aagtctgctg
    28261 atgcctggcg gcgcggtgat taacggcgat ttcaacaatg tgctgccagt ggatttggtt
    28321 gatccgcagc aggtgcagga gtttgtcgat cacgcctggt atcgttatcc caacgatcag
    28381 gtcgggcgtc atccgtttga tggcatcacc gacccgtggt acaaccccgg cgatgtcaaa
    28441 ggcagcgata ccaacattca gcagctgaat gaacaggaac gctactcgtg gatcaaagcg
    28501 ccacgctggc gcggtaacgc gatggaagtg gggccgctgg cgcgcacgtt aatcgcttat
    28561 cacaaaggcg atgctgcgac cgttgagtcg gtcgatcgca tgatgtcggc gttgaacctg
    28621 ccgctttccg gtatccagtc aacgttaggc cgcattttgt gccgcgcgca cgaagcgcag
    28681 tgggccgcag gtaagttgca gtatttcttc gacaagctga tgaccaacct gaaaaacggc
    28741 aatctcgcca ctgcttccac ggaaaaatgg gaacctgcaa cctggccgac agagtgccgt
    28801 ggtgtcggtt ttaccgaagc gccgcgcggg gcgttaggcc actgggccgc cattcgcgat
    28861 ggcaagattg atctctacca gtgcgtggtg ccgaccacct ggaacgccag cccgcgcgat
    28921 cccaaagggc agattggcgc ttatgaagcg gcgctgatga acaccaaaat ggcgatcccc
    28981 gagcaaccgc tggagatcct gcgtactctg cacagctttg acccgtgcct cgcctgttca
    29041 acacacgtgc tgggcgacga cggtagcgag ctgatctccg tgcaggtgcg ttaacagcga
    29101 aggagaatca tcatgcaaca gaaaagcgac aacgttgtca gccactatgt ctttgaagcg
    29161 ccagtccgca tctggcactg gttgacggtg ttatgcatgg cggtgttgat ggtcaccgga
    29221 tactttatcg gcaagccgct accttccgtc agcggcgagg cgacgtatct gttctatatg
    29281 ggctacatca ggttaattca cttcagcgcc gggatggttt ttaccgtggt tttgctgatg
    29341 cggatctact gggcttttgt tggcaatcga tactcccgcg agctgtttat cgtgccggta
    29401 tggcgtaaaa gctggtggca gggcgtgtgg tatgaaatcc gctggtatct gtttctggca
    29461 aaacgtccga gtgccgatat aggccataat cccatcgccc aggcggcgat gttcggctat
    29521 ttcctgatgt cggtctttat gatcatcact ggttttgcgc tgtacagcga acacagccag
    29581 tacgctattt ttgcgccgtt ccgttatgtg gtggaatttt tctactggac gggtggcaac
    29641 tcaatggaca ttcacagctg gcatcggctg gggatgtggc tgattggcgc gtttgtgatc
    29701 ggtcatgtct acatggcgct gcgtgaagac atcatgtccg acgacacggt gatctccacc
    29761 atggtcaacg gctaccgtag ccacaaattt ggcaaaataa gtaacaagga gcgttcatga
    29821 gcgagcaacg cgtggtggtc atggggctgg gcaacctgct gtgggccgat gaaggcttcg
    29881 gcgtgcgggt ggcggaacgg ctgtatgccc attaccactg gcccgagtat gtggagattg
    29941 tcgatggcgg tactcaggga ctgaacttgc tggggtatgt cgaaagcgcc agccatctgt
    30001 tgattctcga tgccattgac tacgggctgg aacctggaac gctgcgaacc tatgccggag
    30061 aacgcattcc ggcttatctc agcgcgaaga aaatgagcct gcatcagaac agtttctccg
    30121 aagtgttggc gctggcggat atccgcggac atctgccagc acatattgcc ctcgtcggtc
    30181 tgcaacccgc aatgctcgac gactacggcg gtagcctgag cgaactggca cgggagcaac
    30241 tgcccgctgc ggaacaggcg gcgctagcgc agctggctgc gtggggaatt gtgccgcaac
    30301 cggctaatga atcgcgctgt ctcaattatg actgtctgtc gatggaaaat tacgaaggcg
    30361 ttcgcttgcg ccagtaccgg atgacacagg aggagcaggg atgagcaacg acacgccatt
    30421 tgatgcgttg tggcaacgaa tgctggcgcg cggctggacg ccagtcagtg aatcccgtct
    30481 tgacgactgg cttacgcaag cgccagacgg cgtggtgtta ttaagcagtg acccgaaacg
    30541 cacgccagag gtcagcgata atccggtaat gattggcgaa ttactgcacg agtttcccga
    30601 ctatacatgg caggtggcga ttgctgacct tgagcagagc gaagccatcg gcgatcgctt
    30661 tggcgtcttt cgctttcctg ccactttagt gtttaccggc ggaaactatc gcggcgtgct
    30721 gaatggtatt cacccgtggg cggaactgat aaacctgatg cgcgggcttg tcgaaccgca
    30781 gcaggagcgt gcctcatgag cgaaactttt ttccatctgc tggggccagg aacgcaaccg
    30841 aacgatgaca gtttcagcat gaatccactg ccgatcacct gtcaggtgaa tgatgaaccg
    30901 agtatggcgg ccctggagca atgtgctcac agcccgcagg tgattgcgct gttaaacgag
    30961 ttacaacatc aactaagcga acgccaaccg ccgttgggcg aggtgctggc agtcgatctg
    31021 ttaaatctca acgccgacga tcgtcacttt atcaatacgc ttctcgggga aggggaagtg
    31081 tcagtgcgca ttcagcaggc tgacgacagt gaaagtgaaa tacaggaggc gatcttctgc
    31141 ggattatggc gggtgcgcag acgtcgcggc gaaaagttgc tggaggacaa actggaggct
    31201 ggctgcgcgc cgctggcgtt gtggcaggcg gcaacgcaaa atctcttgcc gacagattcg
    31261 ctgttaccgc cgcccattga tggcctgatg aatggcctac cgttggcgca tgagttactg
    31321 gcacatgtac gtaaccccga cgcgcagccg cacagcatta atctgacgca attacccatc
    31381 agcgaggctg atcggctttt tctctcacgt ctctgtgggc cgggaaatat tcagattcgt
    31441 accattggct atggcgagag ctatatcaac gccacggggt tacgccatgt ctggcattta
    31501 cgctgtacgg acaccttaaa aggcccgtta ctggaaagtt atgaaatctg cccaataccg
    31561 gaagtggtgc tggcagcgcc agaagatttg gtcgactctg cgcagcggct tagcgaggta
    31621 tgtcagtggc tggcggaagc tgcaccgacg taaaaagacg gtaagtatcg ctttcagtct
    31681 tatgaatatc gcaatcggcg aatacctctg gtcgtagagt ttcaggataa agagggagat
    31741 ctaccattat cgggttattt ttctctcttc gcctacagga gtgcgcatgt gggatgtcat
    31801 tgatttatcg cgctggcagt ttgctctgac cgcgctgtat cactttttat ttgtacccct
    31861 taccctgggg ctgatttttt tgctggctat catggaaacc atttacgtgg tcaccggcaa
    31921 aacaatctac cgcgatatga cgcgcttctg gggtaagctc ttcggtatca attttgctct
    31981 tggcgtggct accggcctga ccatggagtt tcagtttggt actaactggt cattctattc
    32041 caactatgtg ggcgatattt ttggcgcacc actggcgatg gaagcattaa tggccttctt
    32101 cctcgaatcc acctttgtcg ggctgttctt cttcggctgg caacggctga ataaatacca
    32161 gcacctgctg gtgacgtggc tggtggcgtt cggttcaaat ctctctgcgt tgtggatatt
    32221 gaatgccaat ggttggatgc aatacccgac cggtgcgcat tttgatatcg acaccctgcg
    32281 tatggagatg accagcttca gcgagctggt ctttaatccg gtcagccagg tgaaatttgt
    32341 gcacaccgta atggcgggct acgtgaccgg ggccatgttt attatggcga tcagcgcctg
    32401 gtatttactg cgcggacggg agcgcgatgt cgcattacgc tcgtttgcca tcggttccgt
    32461 cttcggtact ctggcgatta tcggtaccct gcaactcgga gacagttctg cgtatgaagt
    32521 cgcgcaagta caaccggtaa aactggcggc gatggaaggg gagtggcaaa cggaacctgc
    32581 acctgcaccg ttccatgtgg ttgcctggcc ggaacaggat caagagcgta acgcctttgc
    32641 cctcaaaatt cccgcgctgc tagggatcct cgccactcac tcattagata aacccgtgcc
    32701 gggtctgaag aatttgatgg ctgaaaccta cccacgcttg caacgcggac gtatggcctg
    32761 gctgttaatg caggaaatat cgcaaggcaa tcgtgagccg catgtgttgc aggcattccg
    32821 ggaactggaa ggtgacctgg gctacggcat gttgctctcc cgctatgcgc cggatatgaa
    32881 tcatgtcaca gccgcacagt accaggcggc gatgcgtggc gcgatacctc aggttgcgcc
    32941 ggtattctgg agtttccgca tcatggtggg ctgtggttcc ctgctgctac tggtgatgct
    33001 gattgcgctt gtccagacgc tgcgtggcaa aatcgaccag catcgctggg tgctgaaaat
    33061 ggcgctctgg agtttgccgt tgccgtggat tgcgattgaa gccgggtggt ttatgaccga
    33121 gtttggtcgt cagccgtggg cgatacagga catcttaccg acatactccg cgcactccgc
    33181 tttaaccaca ggacaactgg ctttctcact gatcatgatc gtagggcttt acaccctgtt
    33241 cttaatcgcc gaagtctacc tgatgcagaa atatgcccgt ctggggccga gcgcgatgca
    33301 gagtgaacaa ccgacgcagc aacaggggta aaggagaaaa tcatgtttga ttatgaaaca
    33361 ttgcgcttca tctggtggct gctgattggc gtgatcctgg tggtctttat gatctccgac
    33421 ggatttgaca tggggattgg ctgtttgctg ccgctggtgg cgcgtgatga tgatgaacgc
    33481 cggatagtga ttaatagcgt tggtgcgcac tgggaaggca atcaggtctg gttgatcctc
    33541 gcaggtgggg cattatttgc cgcctggccc agagtgtatg cagcggcgtt ttccggcttt
    33601 tatgtggcga tgatcctggt gctgtgctcg ctgttcttcc gcccgctggc ctttgattat
    33661 cgcggaaaaa tcgccgatgc acgctggcgt aaaatgtggg atgccggtct ggtcatcggc
    33721 agtctggtgc cgccggtagt cttcggtatc gccttcggca acttgttgct cggcgtgccg
    33781 tttgccttca caccgcaatt acgcgtggag tatctcggca gcttctggca actgctgacg
    33841 ccattccctt tattgtgcgg attgctcagc cttgggatgg tgattttgca aggtggcgtc
    33901 tggttacaac tgaaaactgt tggtgtgatt catctgcgtt cacagctggc gactaaacgc
    33961 gctgcactgt tggtgatgct gtgctttttg ctggcgggtt actggctgtg ggtcggtatt
    34021 gatggctttg tactgctcgc ccaggatgct aacggtcctt ccaatccgtt aatgaaactg
    34081 gtggcagtgc tacctggtgc ctggatgaat aattttgtcg agtcgcccgt tttgtggatc
    34141 ttcccgctgc tggggttctt ctgcccattg ctgacggtga tggcgattta tcgtggtcgc
    34201 ccgggttggg gatttttgat ggcgtcattg atgcaatttg gcgtgatttt cacggcaggc
    34261 atcacgctgt tcccctttgt catgccgtca agcgtgagtc caatctccag cctgacgttg
    34321 tgggacagca cttccagtca gctgacgctg agcattatgt tggtaatcgt gctgatattt
    34381 ttgcctattg tgttgctcta cactctctgg agctactaca aaatgtgggg acgcatgacg
    34441 acagaaactc tccgccgtaa cgaaaacgag ctgtactaag gaacaggaac aatgtggtat
    34501 ttactttggt tcgtcggcat tttgttgatg tgttcgctct ccacccttgt gttggtatgg
    34561 ctggacccgc gtctgaaaag ttaacgaacg taggcctgat gcggcgcatt agcatcgcat
    34621 caggcaatca ataatgtcag atatgaaaag cggaaacata tcgatgaaag cgatcttaat
    34681 cccattttta tctcttctga ttccgttaac cccgcaatct gcattcgctc agagtgagcc
    34741 ggagctgaag ctggaaagtg tggtgattgt cagtcgtcat ggtgtgcgtg ctccaaccaa
    34801 ggccacgcaa ctgatgcagg atgtcacccc agacgcatgg ccaacctggc cggtaaaact
    34861 gggttggctg acaccgcgcg gtggtgagct aatcgcctat ctcggacatt accaacgcca
    34921 gcgtctggta gccgacggat tgctggcgaa aaagggctgc ccgcagtctg gtcaggtcgc
    34981 gattattgct gatgtcgacg agcgtacccg taaaacaggc gaagccttcg ccgccgggct
    35041 ggcacctgac tgtgcaataa ccgtacatac ccaggcagat acgtccagtc ccgatccgtt
    35101 atttaatcct ctaaaaactg gcgtttgcca actggataac gcgaacgtga ctgacgcgat
    35161 cctcagcagg gcaggagggg caattgctga ctttaccggg catcggcaaa cggcgtttcg
    35221 cgaactggaa cgggtgctta attttccgca atcaaacttg tgccttaaac gtgagaaaca
    35281 ggacgaaagc tgttcattaa cgcaggcatt accatcggaa ctcaaggtga gcgccgacaa
    35341 tgtctcatta accggtgcgg taagcctcgc atcaatgctg acggagatat ttctcctgca
    35401 acaagcacag ggaatgccgg agccggggtg gggaaggatc accgattcac accagtggaa
    35461 caccttgcta agtttgcata acgcgcaatt ttatttgcta caacgcacgc cagaggttgc
    35521 ccgcagccgc gccaccccgt tattagattt gatcaagaca gcgttgacgc cccatccacc
    35581 gcaaaaacag gcgtatggtg tgacattacc cacttcagtg ctgtttatcg ccggacacga
    35641 tactaatctg gcaaatctcg gcggcgcact ggagctcaac tggacgcttc ccggtcagcc
    35701 ggataacacg ccgccaggtg gtgaactggt gtttgaacgc tggcgtcggc ttagcgataa
    35761 cagccagtgg attcaggttt cgctggtctt ccagacttta cagcagatgc gtgataaaac
    35821 gccgctatca ttaaatacgc cgcccggaga ggtgaaactg accctggcag gatgtgaaga
    35881 gcgaaatgcg cagggcatgt gttcgttggc aggttttacg caaatcgtga atgaagcacg
    35941 cataccggcg tgcagtttgt aatgcataaa aaagagcatt cagttacctg aatgctctga
    36001 ggctgatgac aaacgaagaa ctgtctaatg cgtagaccgg aagaggcgtt cacgccgcat
    36061 ccggtcactt taccttcagt tttactcttt ctcggagtaa ctataaccgt aatagttata
    36121 gccgtaactg taagcggtgc tggcgcgttt aatcacacca ttgaggattg cgcctttaat
    36181 attaacgcca gcctgttcca gacgctgcat tgacaagctc acctctttgg cggtgttcaa
    36241 gccaaaacgc gcaaccagca ggctggtgcc gacagagcgt cccacgaccg cagcatcgct
    36301 caccgccagc atcggcggcg tatcgacaat caccagatcg taatggtcgt tcgcccattc
    36361 cagtaattga cgcatccgat cgcgcatcag cagttcagac gggttaggtg gcacctgacc
    36421 gcgagtaatc acatcaaagc ctcctttgcc aaaatgctgg atcactttgt tgagctcatc
    36481 tttacctgcc agatattccg acaagccatg ttcattactc acggtaaaca ggttatgcga
    36541 ataaccacgg cgtaagtcgg catcaataaa taacactttt tgatcggact gggcgatcac
    36601 cgctgccaga gttgaactga caaacgtttt accactgtct ggcgtcgcac cggtgatcat
    36661 cagaatgtta ttctccgtct ccatcatagc gaagtgcaga ctggttcgta gcgcacgtac
    36721 ggcttccaca gcagaatccg ccgggttatc caccgccagg aaggggatat ttttagtacg
    36781 atggcgctgc tgattagaaa ataaattttt cttacgcaga cgggagcgtt tatccagcca
    36841 ctcggacatt gggatagtgg cataaacgtt gatgccgtgc tcttccagtt gttccggtgc
    36901 ttctacacca cgacgcaaca tcgctcgcgc cagcacggca cccaccgaaa taaacaggcc
    36961 aagaataaaa ccaagcacca cgttcaacgc ttttttcggt ttcactggct gcggctgagt
    37021 gactgccggg tcgataatcc gcacgttacc aatggcactg gatttcgaaa tactcaactc
    37081 ctgctggcgg ttaagtaatt gcagatatac cgcacggccc gcttctacgt cacgacttaa
    37141 acgcaacact tcctgttggg tggaaggcat agccgatacc cgcttattca ggcgtttgcg
    37201 ttcttgctcc agcgtctggc gtttttccag cagcgcacga taagttgggt gatctttctt
    37261 atacagctgg gagatctctg cctcgcggaa agtcagctca ttgagttgat tatcaacgtt
    37321 cacaatctgc tcaagaacgg ctttggcttc caggttaagg tcaaccgaat cgcgctgctg
    37381 gcgataaacg ttgagttttt cttccgcctg gtccagctcg ctgcgcactt cgggcaactg
    37441 gcgctgtaag aattcaaggc tttgtgaatc ctgcgccgcc tggcgagcga tattctgttg
    37501 caaatagttg ttagcgatgc tgttcagaat acgagtaatc aactggggat catcaccggt
    37561 catggtaagt tccagcatcc cactttcttt actgcgttcg ctaacggtaa aggtttcctg
    37621 caacgcgtta atcgcttcca gttcggtacg ctggctcagg acaaactgtg ttcctggttt
    37681 ggccttaata tccgcgatag tcagcgcaac gccatctttt tccagacgct ggccgaccat
    37741 accattgacg gtgaactctt caccttccag cgtatagtgg ccgttttccc caaccgtgag
    37801 tgtcagttgc tgatcctgac cattcagttg tggaatatgc atccagctga tcgccagctc
    37861 acctggtttc tctttggtta atctcgccca gccgcgaccc acaatcggaa aatacttctg
    37921 ctcaaccatg tcgcgcagat tcagttcagc aatggtttta ccgagaatca tgcgcgattg
    37981 cagcagttgg atctccggtg cagactcggg cgatgagtta gggatcatat cgctcaggcc
    38041 actgagaatg gcgttgccct gtttctgctc aacctggacc agagtatctg cctgataaat
    38101 tggcgtgctt aacagcgagt aagcgacagc gatcagcgtg aataacgcgg tcacgctgat
    38161 aataaactta cggtgatccc ataactcgcc gaccagacga agcagatcga tctcattttc
    38221 ctgagtgctg cctggtggcg tattcatatt tttagttgtc atcgtatttt ataaactcaa
    38281 attaccggct gaggcgcttc gcccattcct gactggcgcg ctccaacata ccgtagacat
    38341 gttcaaatgc gtcctgactt ttacgatagg gatccgggat ctctttctgt tccagccatt
    38401 gcccaaacag cattgtcttc ccgcggacct cgggagcgat tgcggtaacc tgagcaatat
    38461 gttccgactc cattgccaga atcagatcgt aatttcgtgc catctcagca gtgagcttac
    38521 gtccggcatg tccttcaaga gatacgccat gattagcggc aacatctgcc gctgtcgcat
    38581 ctgcagggtg ttttaccaga ccatgaacac ctgccgattt gacttttacg cccggcagac
    38641 gtttacgcag caagcgctcg ccaatgggcg aacggcaaat gttgccggta caaaccacca
    38701 ggattgagtt aaattttagt tgggccatgt ttgaatccac cgtgaagtct ctgtcaggtc
    38761 atgcaccccg gaaatcgtcg gtaccagcag agaaatcaca cggttccaac gagccagcgg
    38821 cgcggtagtg acataaacaa tgtcgtaagg ttcaagctgg aattccgttc cgagtatcat
    38881 tgccgatgcg tccttcgcat tcagctgata aatattggcg attttgccat tctgcttgtt
    38941 ttgcgttgca cgaataacaa aaatccccgt cgcatcagcg acatcctgat tcataccctc
    39001 agcgttaccc agcgcttccg ccagggtcat accgctgcgg tccattttca gcgtgctctg
    39061 tttacccact tcacccatca cgaagacttt cagatcatcg ttgcgcggaa taaataaaat
    39121 atcgcccggg tgcagcagtt tgttctggcg caagtcgcca cgctgcatca gcgcatagag
    39181 gttcactttg gtctttacgc cattctgggt gagcaccacg ttgcgccagt ctgcatctgc
    39241 ggtcagaccg cccgcggcgt taatcgcatc catgatggtc agaggaatat tggtaatcgg
    39301 ctgctgaccc gatttgctga cttcgccggt gacgtacgct ttttgcgatc ggaacgccgc
    39361 gacgctgaca tcgacttgcg ggctttcaat gactgaatcg agacgcgctg taatctcgtt
    39421 gcgtacctgg gtaagtgttt tcccggccac tttcaggcga ccaatatagg ggtagaaaat
    39481 cgcaccatcg gcgttaaccc agttgccggt atcactggcg ctacggtatt gccctgcggg
    39541 ggtggttagt tccgggtgat cccagaccgt aaccatcaac acatcaccaa tcccgatacg
    39601 atattcatag tttgcgattt gctgctccag ctccgggtta gcctgcgaca taatcggttt
    39661 ggcgcgcaac tgatcaatca atcctggcgt gacgggatag atattgacca ttttgtccag
    39721 gtcatactga ttgtcaggta gttcaatgac atccttgtta ctggtcgata aattctgacc
    39781 aggaacaagg gtacatgctg tcaaagacag aacagccaat gtcagtaccg aaaacttaaa
    39841 aatgttcttt ttcattggtt tatatttatc agtcaatgta atgttatgaa ttacgtcagg
    39901 ttattgtcca accggtattt ctcgctctgc cgtcattgga tagagctggt atttgcgtga
    39961 cagcatttgt ccaccatcac gcgtcaatgg tgtccacgaa accaccgcgc ggttgcggtt
    40021 aggtccaatg gtcatcaagt cgaatggaat cgagatataa aaacctttac taaagccgcc
    40081 ttcgccgtag tcatctttag atacgttact gattgccgcc cataccccta ccgccacgcc
    40141 gctgtcaaag cgtttggcga cgtcgatcgt tgcgccttta tcttttgcca gatattgccc
    40201 aacgctaagt ttcatcagta cgccattgag cgtcggcggg ttccagtaag ccgtcacgaa
    40261 acctgttggc gtggaataat cggtgaaacg catcatgttg tcccagtcac gctgcttcac
    40321 gtagttaacg tccacaccca gcgcccagct ggcatctagc gggcgataaa gcagctcgga
    40381 accgacaccg gcatacatcg tttccagata accgccatac acctggccat agaaaccatt
    40441 gcctaagtca gcaaaatagt tcgcctgcaa gttgttgaga taaacgtcat tgcgaacgta
    40501 gtcacggata tgcgtgcgca cgcggggcag ggtagagtcc gcgggcaaca gcgaagactt
    40561 aaacttgtcg tagttgttgt aaatattggt gaaaataccg ccatcaagca gcaggtggtc
    40621 ggtaaaccag tagcgggcac tggacatcag ccccagctgg aacatataga aatcttccgg
    40681 gccgcccagc gactgtgaaa gtgttgggtt gaaagagtag ctaaagcgat cttcacgaat
    40741 acggtagccc cgaccaaagg cagaaagatc ttctgcttca acacgttgtt gttgcagttg
    40801 ctctgattga ccaggcgctg taccagccag ctgtttgcgc aggctggcta cgtcggtttc
    40861 ggtagtcacc atcgccatat gctcgcgctt ttgcgtcacg ctaatcttct caacgccttg
    40921 cggcaggtta ttcaccagaa tccggttggc acgatcgacc gcttcacgag agtcacggta
    40981 tttgtattgc tgaccagaca tatacagcgt cttatcgcgt agctgaattt ctggcgcgtc
    41041 aaagcccgcg ttatacttca gggcggtaag ttggtttgct accgtggtgt actgcaatcc
    41101 ttcagattca ggcgcaggtt gatatgccgg ttttggcgta tcgcgcaggg cagggcgcag
    41161 atcgttgaaa ttggtccgta aagtgaaacc aaacatcaac gtgttaccgc gttcataact
    41221 caggttgaga tctgcccagc tggcagcgcg ataaactgca ccgacgttga aatggcttgc
    41281 ctgaggcagt ttgccagcga aatcattctg gtaattgttg ccgtcgtatt cgagtttcag
    41341 acgcagggga ttccacggcg tttgatactc aatgccgcca aagatggaag ccgggccacg
    41401 aaagatatcg ctaaagctga tatcgcccgc atcgtgagac tctgcgcgat gacaatattt
    41461 atcgctcacc cggcaaaacg ggttggtaat attgcccgca ttaccggcgt agccccatgc
    41521 catcccgagg gtgaaatcaa atggccccgc ttgcttgctg gccaccagat actcaccgtc
    41581 aaacagaccc gtaccagcaa tatcacgttt accaaacgcc acttgcggta gccagtgacc
    41641 ttcttcccac aggcgaagtt taaaatcgaa tgatttgtct ttgtatgact gatcgccgct
    41701 gaaatcttcc cactggctat atttgcgtgt gcgcacatcc gtataacgaa tggtgccttc
    41761 cagccatggg aacagcgcca cggaggtgga gtagaaccgg tattgatcgt tatcccggta
    41821 gttgacgctg aattcacctt ccggtgcgat gcgcgcattt ggcatctgca acaatcctgt
    41881 gccgccaaag tctgattgcg acgaacccag cggatccggg taggttaata cttcagcatg
    41941 acaggcactg gagacggcaa tggccaggca gcttaaaaga taagaattct tcttcatcag
    42001 tcaggaacac gttgcgttaa aacagacaca atctggtcat ttaaatcagc atatttttcg
    42061 ggcagtacat gcgcagaaaa gcccaaccaa agctggctgc caggcggtgg ttccacatgt
    42121 cttttattcc acagggcaac aggggcgacg acagtctcac cctcaggcgt aatcaccata
    42181 acgttattct tgtccgcgcc tgcaaggcgg gggtgatctt gcagataatc ggtaacgcta
    42241 cggcctgcct gccagggcaa ttgcccggcc ccagaaaccg cacccagcag agtaatagtc
    42301 accggacgct gtacggtata gagcgtatag tccccgacca acgggggatt gctgttctca
    42361 tccactcgta caaaatcagg gtccagtttg accggtaagc gaccagtgat attgagattg
    42421 agtaactgct ggcgcacgga tttaatcgtt gccgccacat cgtcgtcagc ttctgcttcc
    42481 caggaagcaa gctgtgccat gacatgttgg tagtctttaa gcgctttagc tttcgccgca
    42541 gagtcggtca gcaaggcacc aggccaccag aggcgatcgc gcagttgcgg ttgtgtcacc
    42601 aactggacaa cattttccac ggggccaacg gaaagtgtct gttgttcgcc aggcagataa
    42661 atagtcaccg ttccttgcgc aaaggcatgg ggtgtcatta cgtaaagtac gctggcaatg
    42721 aaatacgact gtaatttatt catggtgcgg gtttaagaaa cgtcatttct acgggaataa
    42781 cccctgcgcc taacatttgc cgactttgac gaacctggcc agtagccgaa tcgacccaga
    42841 atgtgttttg ccactgtttt tctggccgcg tggaagtcac ctcttcctgc cagacattac
    42901 acagcacatt gcgacccgca atattcagcg tctctaatcc agcaaaggaa aaagtagaac
    42961 tcagggtcgc agagcgaaaa tggttgtctt cactccagag aatatcgcgc gtccagtggc
    43021 tgccatcttt tattgccagc gcgttacgca gagggtcctg cccggaattt gtcacttcca
    43081 gcagattatt attaagtttg acggttttta aaagacgtcc attgtgggta accagcatgg
    43141 cgttatcctg ggataaccat ttgctttgtt cttgttctat atatcccagt accacaaaga
    43201 ttcgttgccc ttcattaagg cgtaaataca tcgtggaata aggcaacgcc tggatctgct
    43261 gatccgctac ggtgatatcc tgattatcga aaaggctggc gcgaaatgta tcgaccatac
    43321 tttgctggct gtgcgtacat cccgcaagaa atagtgcgaa aatcgataga ataagagggc
    43381 gcacctttaa tccttgaaag gttaattaaa aaaatgcaaa tctctaaagc ccggtacgaa
    43441 tatcgcaacc gggtatcatt taactttaat ttcgacgcgt taaagcagta ttagcgtgta
    43501 gtggacgtgg tggttgaggt gttatgagaa ccgccatcgc cactgtcatt tgctgcaaca
    43561 actgccaacg tcgccacacc aacagccgtt gccacaccga caaccgtcgc cgtgccgaca
    43621 gcgccagcag ccacgccacc agtggtagcc gcagtcgtgg ttgcaccggt agtggtcgtt
    43681 gtggtgccag tagtacccgt cgttgcctcg gtcccgtttg ctgcttcagg ggcagcaaat
    43741 gcagctggcg tcgtcaacat gaaggccata aggattgcag aaagcttgtg tttcataact
    43801 tttcctttat tcatcgcatg gacaatacgt acgcttgaga tcaggattgt ctactttgag
    43861 tataacatcc ttgtgaaatc tccctttcct cataccgctt gcactatata ctgcgggtag
    43921 gaaaggcgcg cagagggaaa tataagattg ttaactatcc ccctctggaa atacgacttg
    43981 tgagcataag ataaatctta gcaaaataca aattaaagat aaggaaattg ttacgaaagc
    44041 tattaattat tgttggtaat atagtttcaa gcggtactat aactggctgt taaatggcat
    44101 ttgtaactat taggtgcaga gatattcgct taaatggatt agtttctttg tggaaaagta
    44161 actgatgtta taatgtatga tgttgactat tgcactattc caataaaacc agtcagcttt
    44221 aaacaagcag cgtcatatta agagagataa acatttgccg ctgttggtcc tcgcaggcca
    44281 tttacgcggc aaaattccac gcgtaatcct ggtataagca cttctgcgtc gcggggagtg
    44341 aatgcggaaa tatggacctg gacttcttta cgaccgtcgg aggggataat gaatcctttg
    44401 ccgcttttgc gatcaaaggt tttgacaatt cctgtcattt tacgggacaa acaaattcct
    44461 tactgaaaat actgtgccgc actatacggg gttaataaaa taaagccagc gatatttaag
    44521 accgccggac ggctaaaata aaatttgctt aatctcaatt atcatgcgtt aatagctgcg
    44581 tcggtttgaa agacagacag catacaaagt agtttactaa agcagttctc attatcaggc
    44641 attatccctt tcttttgagt ctctctcctg aacactaagt agtttctgta ttaaagccct
    44701 gtttgccgaa aggcccaaaa tgaaggaagt aaaatatgtc taataaaatg actggtttag
    44761 taaaatggtt taacgcagat aaaggttttg gctttatcac tcctgatgat ggcagcaaag
    44821 acgttttcgt ccatttcacc gccattcaga gcaatgaatt ccgcacgctg aacgaaaatc
    44881 agaaagttga attttctatt gagcaggggc aacgtggccc cgcggcagcg aacgttgtta
    44941 cgctctaagg ttgccgttat tactcaacat ctccatttcc gctgtccatg ttgtcatggt
    45001 tcacagtacc gtacatcggc attcgatgtg acggagcgaa accctttggg cgctaagtgt
    45061 attttttgta aatcgacgat gatcaccttt gataacgtcg cgctgcaaat acgcacggac
    45121 catgcgccgc tggatttcac aaaataatat caggctccct cgtggagcct tttttatatc
    45181 tgccttattt ttcttcaacg ctgtatgtat agtaagcgat aacctgttga ttattgaatc
    45241 tttcggggag atggcttata acatttctta cctgaccagg gtaccgggaa ccaacacctt
    45301 actggcgtgt tgctgtcttt caagaccaga cgaggttaac agtgaatatt gaagagttaa
    45361 aaaaacaagc cgaaacggaa atcgctgact ttatcgcgca aaaaatcgcc gagctgaaca
    45421 agaatacagg gaaagaagtc tctgaaattc gcttcaccgc acgagaaaaa atgaccgggc
    45481 ttgaaagtta tgatgtcaaa atcaaaataa tgtgattttg tgaacatcac cccgtgcgag
    45541 gtgatgttcc gcttgttgct aatttagtga ccaatcattg gcgcttgtgg aattaagcgt
    45601 cggtacaatt cctccggcac cgggctttgc catactcccg catacattgc gtaaccaatc
    45661 accgcaaaca taatccccag aaccagtagc gtcattaacc agccagacaa cgcaaaagct
    45721 tttttatttg ccgcaggttt ttgcagtgaa aaggtcaatg ttgaggctac cggacatgac
    45781 tctacgcaag tcatacagcc ggtacattcc actgttcgta cctgaattaa tttatctacc
    45841 gggatccgtg atgggcaatt ttttgcgcat ttgccacagt cgatacaact ttcggcattg
    45901 cgacgaatct taaacggcga caatagcgaa accacgccca tcagcgcgcc atatgggcaa
    45961 agataacgac accaggcatg gcgaataaac aggctggcaa tcagcaaaac ggtcacgctg
    46021 attaatgtcg cggtccccat atgacgaaag aaatcgagca tttttacgtc catcaccacg
    46081 ctgtagggcg acaacataaa atagtgaatc gcctgagcgg gcatcaataa cgcgatatag
    46141 agaaaaaaac tcaacagcaa atacttcacg ccgcgcagag gaatatccag ccagcgggga
    46201 aggacacatt gccgaccaaa cagtttgtta ccgagatcgc cgattaattc agaaagcgta
    46261 ccaaccgggc ataaccatga gcaaaaggcc tttttgagta atagactgat gacgataaaa
    46321 gcgaccaata acagcatcgc ggcgacgtgg acggacggta actgacctgt tgtaaggcta
    46381 tatttcagat tcatcagacc ggcaatcggt agccagcctt cgatacctcc cggtctggcg
    46441 acaaatgtcg tgctacttgc cgtttcgtaa tagcgcaccc aataccagaa cgtgatggca
    46501 atataaatat tcattgccaa cagtaataat tgcgtcgctt tacgccaggt cgtggcatta
    46561 cgccagtcat tccacggtaa tttgccgccc gtcgtgcctg gccgccgctg ccagcgggtt
    46621 cttttattct ctgccatgat tttgccagtc cgttaagttg tataccaaat gccactattc
    46681 tagttgttct taactggctg atattgattc aaatcgcgtt caggtctttc ttatgcaacc
    46741 atgcttccag agcggcaaca ctgcgtgtaa tttcttcgtg tggaaggggg gcagataatg
    46801 gctgctgctc cagttgtgcg catagctggc tggcgatacg cattcccaga ctcgagcaac
    46861 tgctttttag ctgatgcgcg gcacgcttta ttttctcgct atcctgactg gcgcgggcaa
    46921 tgtcgatttc atcgagaagc ggcagggcat gttgtgtaaa taataccagc cattcgtgga
    46981 tcttctccgt ccccattaac tgagcatctt cattgagttg cgatacatcc agcgattgat
    47041 cattattgac ttgcagttgg agatagtgcg ccagtaactg accgagcact tcacgcggca
    47101 ccggtttagg gataatcccg cggaataatg aactggtacg ctggcgcagc gtttcgtcaa
    47161 tgacatgggc gctaaagcca atcaaaacca gcgacggata ttgctgtgct agttgtcggg
    47221 caagcgtaat gccgtcgata tccggcagat caaaatccac cagtgcggca gcaaacggtt
    47281 cgctattttg cagtgtctct aaagcctgcg cggcattgcc aacagcaaca atctgcgcac
    47341 cactggtttt cagcatctca atggtaattc gctgggttag cgggttatct tcaattaaca
    47401 gcaaacgtaa accgtcaaga cgcaccgcct gattgactgt ttttggcacg ggtgccgtgg
    47461 caacacgtaa cggcaagcgt aaacaaaaac agcttccaac ctccggcgtg ctggtggcgc
    47521 tcagttcgcc gcccatcgcc tgggccagac ggctactgat agtcagtccc agcccggtgc
    47581 cgccgcgttt gccgcttacc tgcacaaatg gctggaagat ttctgccagt ttcgcgggat
    47641 caataccgca gccgctgtct tccacttcga ccagccattg ctcgccatca gtgcgactac
    47701 gcaggataat gtacccttcg tcagtaaaac gcagggcgtt gctcaacagg ttggttataa
    47761 cctgacgaat acgtcgtgga tcgcccatta acgcgcacgg catatcatcg gcaattgccg
    47821 ttgccaggcg aatcgggcga cctttcaccc gtccgctcat taattgcagg gtactttcca
    47881 gcagcgggcg cggttcaaag ggctcatcgc tgaccgaaac attcttgcca cctgcttcga
    47941 tagcggaata atcgagaata tcgttgagga tggtcagcaa cgattcacca gagtcagtaa
    48001 ttgcccgcaa atcatcacgc tgggcgttaa gtgcggggtt atctgccagt agctgtgcgg
    48061 tgccgagaat accgtacagc ggtgtgcgga tctcatggct catcgccgcc agaaacgccg
    48121 attttgcctg gctggctttt tctgcttccg cccgtgcctg tcggtgttct atcaccagtt
    48181 cctgcaattc agctgtacgc gctttgacct gcgccgccag ctgttcgcgg tggcgattca
    48241 gtgcatgaac attgctgcga aacgcatcca tcagccgccc gatggtatcc agctcccgta
    48301 cgccagcggt ttccgggaaa ggggagtcaa tatcaccgtc cagcagccgt tgcagcgcct
    48361 gcgtttgttc ggcaagtgga cgcgtgactg agcgataaac cacgcgccag aggatcagaa
    48421 tcagtgcgca aagtgaaacc atccccagca ataacaggct gtattgcccg cgtgcactgg
    48481 ctttttccag atgtgccagt ccatgctgat tgcgcagctc aatggtgtcg accagctgac
    48541 tgacttcgct actaaactgc gcgaactggg cgatattatt ttgggcgaga gtttgcaggt
    48601 gattgctgat ttcactgtcc tgctggaaca gcgccaacag atcgctatat tggctaacgg
    48661 tagttaacgt tgttgcgacc tgcgcacgaa cacccggatc ttcaatgcgt atttgccgac
    48721 gttgcagaat tttcaccgca ttattgagct gcttttccag cgttggcgca tttttctgga
    48781 tctgctccag ccccagattc atcaccattt gctgcacccg cagggcgcta aggcgcagtt
    48841 cattcatctg gttaacatac tcaagatcga tatcaatcag ccgatcgagt gcactttctg
    48901 cggcctgacg ctgatcttgt tcgatcaaat cgtaaatccc ggcctgggtc gctccggcgg
    48961 aggttgccgc attattcgcc tgaccttgcg ccaggcgtgc gatctcatcg gcggcagcga
    49021 ctatctgctg actgagttgc tgttgttgct ggcgcagttg caaacgctgc cccaccagtt
    49081 ccccttgctg acgtaacgaa cgggagatct cctgctcctg ttgttcaata gcggtggtat
    49141 caaaaccttg ttcccgtaac gcttgcagca acgcattaat cttcaggctt tgtgcggtga
    49201 gcattcgccc ctgcgcctgc cacatctttt cgttatcggc actggtcagg ttctgcgcgg
    49261 cgaaaagttc ccaggcgctg gcttcgctca actggcgcgc catattcatg gtaggaatca
    49321 atgcctgagt attgtctttt tccacctggc tgataaagcg caggttgtac catcccacca
    49381 gggtactggt cagggttaac agcgccatca gggcaaagcc catccagagt cttcgggtca
    49441 gggttaaatt cacggtcggt gcactttagg tgaaaaaggt tgagtcgcaa agcggaatgc
    49501 atctagcata aagccttatt attgatgagg ctatcatgcg cgtactgcta tttttacttc
    49561 tttccctttt catgttgccg gcattttcgg ctgataacct gttgcgctgg catgatgcgc
    49621 agcatttcac ggtgcaagcc tctacgccgc ttaaagccaa acgcgcatgg aaactgtgcg
    49681 cgctttatcc cagcctgaaa gattcatatt ggttatcgtt gaactatggt atgcaggagg
    49741 ctgctcgccg ctacggtgtg gatttaaaag tgctggaggc aggcggctac agccagttgg
    49801 ctacccagca agcacaaatc gaccagtgta aacagtgggg cgcagaggcc attttgctcg
    49861 gtagtagcac gacctcattt cccgacctgc aaaagcaggt agcaagtctg ccggtgatcg
    49921 aactggtaaa tgctattgat gctccccagg tgaaaagccg cgttggtgtg ccctggtttc
    49981 agatgggcta tcaaccgggg cgatatctgg tgcaatgggc gcacggtaaa ccactgaatg
    50041 tgctgttgat gcccggaccc gataacgccg ggggcagtaa ggagatggtc gagggttttc
    50101 gcgcagccat tgccggaagc ccggtgcgta ttgttgatat tgcgcttggt gataacgata
    50161 ttgaaatcca gcgtaacctg ttgcaggaga tgctggaacg ccatccagaa atcgacgtcg
    50221 ttgccggaac ggccattgcg gcagaggcgg caatggggga agggcgtaac ctgaaaacgc
    50281 cgcttaccgt ggtgtcgttt tatctttcac atcaggtgta tcgcgggctg aagcggggaa
    50341 gagtgattat ggctgccagc gatcaaatgg tctggcaggg ggaactggca gttgagcagg
    50401 ccatcaggca attacagggg caatcggtgt ctgataatgt cagcccaccg attttagttc
    50461 tgacgccgaa aaatgccgac cgagaacata tccgccgctc gctgtcaccg gggggatttc
    50521 gtccggtcta tttttatcag cacacatcag cggctaagaa ataaccttca ccatgttgcg
    50581 tcaccagtaa atccgcgctg agtttatgac gtaaacgacg aattaacaca tcgacggtgc
    50641 gcaggtcagg gttttccacc cgacgcgcag aaagcatacg tagcagacgt tcacggctga
    50701 gaatttcgcc cggattcgtc acaaatgcca ccaacatttc atactctgcg cgggtcagtt
    50761 taatcggctc gccatcccgc tccagcgtat ggcgcgacac attcaggcaa taaccggcaa
    50821 agcgatagca gttgtcctga gtgtgcggtt gagcttgtcg cgcgaggtcg attcgccaga
    50881 gcagattttt cacccgtact accagttcgc gcagttccag cggtttggtg acgtaatcgt
    50941 ctgcgcccat ttccagccca acaatacggt caatccgatc gctgcgtccg gtaaccagaa
    51001 taatccccac cgttgagcgt tctcgcaggg cgcgggttaa catcaggcca ttttcatcgg
    51061 gtaagttgat atccagcaga attaaatcta ccggctgatt ctgcataatt tcccgcagcc
    51121 cggcaccgct cgctgtaacg gaaacggtat acccctcctg agtgaagtaa gattgtaatc
    51181 gcgcctgggt aaccggctca tcttcaacaa taacaatgtg atgtggcatc agagggtttt
    51241 actcattctg ttcatatctg ttcatattct gccgtaagcc gttcatcctg accagtgccg
    51301 ctgttcatat ttgctcatta agatcgcttc actaaaccat aattttacag gggctattat
    51361 gcggaaactc tggaacgcgc tacgccgacc cagtgctcgt tggtcggtac tggcgctggt
    51421 cgcaattggg attgtgattg gcattgcgct gattgtattg ccacacgttg ggatcaaagt
    51481 caccagcaca accgaatttt gtgtcagttg ccacagtatg caaccggtgt atgaagaata
    51541 taaacagtcg gtgcatttcc agaacgcctc cggcgtgcga gctgaatgcc atgactgtca
    51601 tatcccgccg gatatgccag gcatggtgaa gcgtaaactg gaagcgagca atgatatcta
    51661 ccagaccttt attgctcact ccattgatac acctgaaaaa ttcgaagcca aacgcgcgga
    51721 acttgccgag cgtgaatggg cgcgaatgaa agaaaacaac tcggcaacct gccgctcctg
    51781 ccataactac gatgcgatgg atcatgcgaa gcagcatcct gaagcggcac gtcagatgaa
    51841 ggtggcagcg aaagataatc aatcctgcat cgactgtcat aaaggtattg cccaccagtt
    51901 accggatatg agtagcggct tccgtaagca gttcgatgag ctgcgcgcca gtgctaatga
    51961 cagtggtgac acgctgtact ctattgatat taagccaatt tatgcggcga aaggcgataa
    52021 agaagcctct ggttctctgc tgcctgcttc ggaagtgaaa gtccttaaac gtgacggcga
    52081 ctggctgcaa attgaaatta ccggctggac ggaaagcgcc ggacgtcagc gtgtactcac
    52141 ccaattccca ggtaaacgca tctttgttgc ctcgattcgt ggtgatgtgc agcagcaggt
    52201 aaaaacgctg gagaaaacca ccgttgccga caccaatacc gagtggagca agttgcaggc
    52261 cactgcgtgg atgaagaaag gcgacatggt gaacgatatc aaaccgatct gggcttatgc
    52321 ggattcgttg tacaacggca cctgtaacca gtgccacggc gcaccggaaa tcgcccactt
    52381 tgacgctaac ggttggatcg gcacgctcaa cggcatgatt ggctttacca gtctcgataa
    52441 acgtgaagaa cgcaccttgt tgaaatatct gcaaatgaat gcgtctgaca ccgcaggtaa
    52501 ggctcacggc gataagaagg aagaaaaata atgaacaata acgatctctt tcaggcatca
    52561 cgtcggcgtt ttctggcaca actcggcggc ttaaccgtcg ccgggatgct ggggccgtca
    52621 ttgttaacgc cgcgccgtgc gactgcggcg caagcggcga ctgacgctgt catctcgaaa
    52681 gagggcattc ttaccgggtc gcactggggg gctatccgcg cgacggtgaa ggatggtcgc
    52741 tttgtggcgg caaaaccgtt cgaactggat aaatatccgt cgaaaatgat tgccggattg
    52801 ccggatcacg tacacaacgc ggcgcgtatt cgttatccga tggtacgcgt ggactggctg
    52861 cgtaagcgcc atctcagcga tacctcccag cgcggtgata accgttttgt gcgcgtgagc
    52921 tgggatgaag ccctcgacat gttctatgaa gaactggaac gcgtgcagaa aactcacggg
    52981 ccgagtgcct tgctgaccgc cagtggttgg caatcgacgg ggatgttcca taacgcttcg
    53041 gggatgctgg cgaaagctat tgccttgcat ggtaatagcg ttggtacggg cggagattac
    53101 tctaccggtg ctgcgcaggt gatcctgccg cgcgtggttg gctcaatgga agtgtatgaa
    53161 cagcaaacct cctggccgct ggtattgcag aacagcaaaa ccattgtgct gtggggctct
    53221 gatttgctga aaaaccagca agcgaactgg tggtgcccgg atcacgatgt ttatgaatat
    53281 tacgcgcagc taaaagcgaa agtcgccgcc ggtgaaattg aggtcatcag catcgatccg
    53341 gttgtcacat ccacccatga gtatctgggg cgcgagcatg tgaagcacat tgcggttaac
    53401 ccgcaaactg atgtgccgct gcaactggcg ctggcgtata cgctgtacag tgaaaacctg
    53461 tacgacaaaa acttcctcgc taactactgt gtgggttttg agcagttcct gccgtacctg
    53521 ctgggtgaga aagacggtca gccgaaagat gccgcatggg ctgaaaaact gaccggcatt
    53581 gatgccgaaa ccattcgtgg gctggcgcgg cagatggcgg cgaacagaac gcaaattatt
    53641 gctggctggt gcgtgcagcg tatgcagcac ggtgaacagt gggcgtggat gattgtggtt
    53701 ctggcggcga tgctggggca aattggcctg ccaggtggtg gctttggttt tggctggcac
    53761 tataacggcg caggcacgcc ggggcgtaaa ggcgttattc ttagtggttt ctccggctct
    53821 acgtcgattc cgcctgttca cgacaacagt gattacaaag gctacagcag caccattccg
    53881 attgcccgtt ttatcgatgc gatcctcgaa ccggggaaag tgatcaactg gaacggtaaa
    53941 tcggtaaaac tgccgccgct gaaaatgtgt atttttgccg gaactaaccc attccatcgc
    54001 catcagcaga tcaaccgcat tattgaaggc tggcgcaagc tggaaacggt tatcgccata
    54061 gataaccagt ggacctcaac ctgccgcttt gccgatatcg tgctgcctgc gaccacgcag
    54121 tttgagcgta acgatctcga ccagtacggc aaccactcca accgtggcat tatcgccatg
    54181 aaacaggtgg tgccgccgca gttcgaggcg cgcaacgact ttgatatttt ccgcgagctg
    54241 tgccgtcgct ttaatcgcga agaagccttt accgaagggc tggacgaaat gggctggctg
    54301 aaacgcatct ggcaggaagg tgtacaacaa ggcaaaggac gcggcgttca tctgccagcg
    54361 tttgatgact tctggaataa caaagagtac gtcgagtttg accatccgca gatgtttgtt
    54421 cgccaccagg cattccgcga agatccggat ctcgaaccgc tgggcacgcc gagtggcctg
    54481 attgagatct actcgaaaac catcgccgat atgaactacg acgattgtca ggggcatccg
    54541 atgtggtttg agaaaatcga acgctcccac ggtgggcccg gctcgcagaa gtatccgttg
    54601 catctgcaat ctgtgcatcc ggatttccga cttcactcgc agttatgtga gtcggaaact
    54661 ctgcgtcagc aatatacggt agcgggtaaa gagccagtgt tcattaaccc gcaggatgcc
    54721 agcgcgcgcg gtattcgtaa cggtgatgtg gtacgcgtct ttaacgctcg cggtcaggtg
    54781 ttggcagggg cagtagtttc tgaccgctat gcacccggcg tggcacgaat tcacgaaggg
    54841 gcatggtacg atccagataa aggcggcgag cctggtgcac tgtgcaaata cggtaacccc
    54901 aacgtgctga ccatcgacat cggtacttcg cagctggcgc aggcgaccag tgcgcacact
    54961 acgctggtgg aaattgagaa gtacaatgga gcagtggagc aggtgacggc gtttaacggc
    55021 cctgtggaga tggtggcgca gtgcgaatat gttcccgcgt cgcaggtgaa atcatgacca
    55081 cgctgacagc acaacagatt gcctgtgttt acgcctggct ggcgcagttg ttctcccgtg
    55141 aactggacga tgaacaactg acgcaaatcg ccagtgcgca gatggctgaa tggttttcgt
    55201 tgctgaaaag cgaaccgccg ctggctgcgg cggtgaatga gctggaaaac tgtattgcca
    55261 cgctgacagt acgtgacgat gcccgtctgg aactggccgc tgacttttgc ggcctgtttc
    55321 tgatgaccga caaacaagcg gcgctgccgt atgcatcggc ctacaaacag gacgagcaag
    55381 agattaaacg cttgttagtt gaggcaggga tggaaaccag cggcaatttc aacgaaccgg
    55441 cagatcatct ggcgatctat ctcgaattgc tcagccatct gcatttttcg ctgggagagg
    55501 ggaccgttcc tgcgcgaaga atcgacagtt tgcggcaaaa aacactgacg gcgctgtggc
    55561 aatggttacc agagtttgtt gtgcgttgtc gtcagtatga cagctttggt ttttacgcgg
    55621 cactaagcca gttattgctg gtgttagtgg agagcgacca ccaaaacaga taacgtcgtt
    55681 tgtgcgcctg aaaagacgcg tttagcgtcg catcaggcat tatggggcag ttgccggatg
    55741 cgtcatcaca tccggcaaac acacatcacg gatgagctac aaaccgggaa agtcgctggc
    55801 gcagcaggcg gttttcctgc ttcaggtgcg caatatcatc cattaacgtc agcgccaccg
    55861 cgatcccagg ccagtccaga gccagttcat gacgcaggcg tactgcgcgt tgcaccacga
    55921 tggcggcatg gtcgtcaaat acccaggttg tttcctggat ctcacgcggt tcaaccaccc
    55981 ccaaaccgac aatttcattc aactcctctt cagagatgcc ggtatgcagg caaaattcgg
    56041 taatagtaaa agtcaccgta acattagcca ttatgctttc ccccaatctt tacgtggatc
    56101 aaaagacgac tgggcgtctg ccagttgctg ccacagcgcg gcagtgtttt catccggttt
    56161 cggcggcatc acgattttca gtaccgcata cagatcgccg gtctgttttt tgctcaccag
    56221 acctttgcct ttaacgcgca atcgttgccc ggcctggctg cctggcggga tagtcagcaa
    56281 aatgctttct ttcagtgttg gaacggtgac tttagcaccc agcgccgctt cccacgggct
    56341 aaccggcact acaatttcca gatcatggcc gacaatatcg aacagcggat gtggcgcaat
    56401 atgaatcacc agccacaaat cgccatttgg accgccgttt tcgcccggcg tcccctggcc
    56461 tttcagacgg atgcgttgac cattgccgac gcccgccggg atcttgacat tcagcgtttt
    56521 cggaatttcc tgttcgatca tgccaaaggc gttataaacc ggcaggttat agctgatggt
    56581 acgcttatgc tcagtaagcg tttcttcgag gaataccgcc acttcgattt caatatcgtg
    56641 gccgcgtgtg gcggggcgtt gacggctctg gcgggcatgc tgaccgaaaa ttgacgagaa
    56701 gatatcgtca aaatcttcgg cgttaaaact ctgaccgtcg ctatgatgga actgacggtt
    56761 aaattgcgga tcgttgcgat gttgccacat ctgatcatac tcagcgcgac gttgttcatc
    56821 acttaacact tcccaggctt cagcgacctc tttgaagcgg gcttcggcat ccggttcttt
    56881 gctgacatca ggatggtatt tgcgggccag tcgacgatag gcggtcttga ttgtcttgag
    56941 atcgtccgtc ggtttcacgc ccatgatggc gtaataatcc tttaattcca tagcgttatc
    57001 tcgcgtaaat caacacaaat tgaaggaacc cctgtaaggt aactcctata agtgtagggt
    57061 aatcctcaaa atttcatatg ccaacacaga atatgttatt gaaatcatcg cggagaggag
    57121 gtcgcctcaa ggtggggtgc tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt
    57181 tgatagtgga accaggcact ttaagtttaa ctggggcgtc attatttatt aaattttata
    57241 ggcgctatat atgagtagta atatacatgg aattagttgc actgcaaata attatttgaa
    57301 acaagcctgg aacaatataa aaaatgagca cgaaaaaaat caaaaatatt caatcacgct
    57361 ttttgaaaac acactggtgt gttttatgcg gttgtataaa gaaatcagac gtcaaaaagc
    57421 tgaagactac attccatgcc tggaatgcga ttcactagaa aaagaatttg aggaaatgca
    57481 gaatgataat gatttatcat tatttttgag aacattgcgt actaatgata cagaaactta
    57541 ttcaggggtt tcagaaggta ttacatatac tatacaatat gttcgagata ttgatattgt
    57601 tagagtgtcc ttgccgggca gaggttcaga gtctatcaca gattttaaag gttattattg
    57661 gtatggcttt atggaatata ttgaaaacat taatgcgtgt gatgatgttt tttctgagta
    57721 ttgtttagat gatgaaaata tgagtatcca gccagaatgg ataaatacgc cgggaatatc
    57781 tgatttagat actggtattg atttgtctgg tatatctttt attcagagtg aaattaataa
    57841 aacttatgga ttaaaatacg ctccagtaga tggcgatggc tattgtctgt taagggccat
    57901 actggtttta aaagaacatg agtattcatg ggcgctgggc agccataaga cgcaaaagca
    57961 agtatacgaa gagtttatta aaattgttga taaacaaacg atcgaggctc ttgttgatac
    58021 ggcattcaat gatctcaggg aagatgtaaa gacattattc ggcgttaatc tacaatctga
    58081 caacaaaatt caggggcagg gtggtttttt atcatggagt tttttgtctt ttaaaaaaga
    58141 atttattgat agttgcttga atgacaaaaa atgcatactg catttacccg agtttatatt
    58201 taatgataat aaggcacgcc ttgttttaga caccgaccca gagcagaagg ttaatgaggt
    58261 gaaaaatttc ctcactgctc tttcagatag catttgctca ttatttattg ttaatagtaa
    58321 tgtggcatca atctccttag ggaatgaatc cttttcaaca gatgacgacc ttgagtatgg
    58381 gtatttaata aacactggca atcattatga cgtttacctc cctcctgaac tttttgctca
    58441 ggcttacgag ttaaacaata aggaaaggaa tgcgcaaatt gattttttaa ctcgttatgc
    58501 aatttactga caaaggcatg tgctaaaaac cattgttatt agcctcactt ctcttattat
    58561 tggtactatt gcctctgtat tagtaacgcc gctgatattc tgtttagcca caggtgtaat
    58621 catcagcggc gtacgcgagg cagggagcta atcaggcata gtttgcgtca aaccttgcct
    58681 gtttttaaag atgtatatag aaaaacaggc attcaacaag ccattttgcg aacctgttcc
    58741 cggaaaaatg tcatatttct gtcacactct ttagtgattg ataacaaaag aggtgccagg
    58801 aatgaacaaa acgctaatcg ccgcaactgt ggcagggata gttttactcg cttcaaatgc
    58861 tcaggcacaa accgtaccgg aaggctatca gctacagcaa gtgctcatga tgagccgcca
    58921 taacttacgt gcgccgctgg cgaacaatgg cagtgtgctg gagcagtcta cgccgaataa
    58981 atggcccgaa tgggacgtcc ccggtgggca actcaccacc aaaggtggcg tgctcgaagt
    59041 gtatatgggc cattacatgc gtgaatggct ggcacagcag gggatggtga aatcggggga
    59101 atgcccgccg ccggacaccg tttatgccta tgccaatagt ctgcaacgta ccgttgcgac
    59161 cgcacagttc tttattaccg gcgcattccc ggggtgtgat attcctgtgc atcaccagga
    59221 aaaaatgggc actatggacc caacctttaa cccggtgatc accgatgatt ccgccgcatt
    59281 cagtgaacag gcggtggcgg caatggagaa agagctcagc aaactccagc ttaccgacag
    59341 ctaccaacta ctggaaaaaa tcgttaacta taaagattcc cctgcctgta aagagaaaca
    59401 acagtgttcg ctggtggatg gcaaaaatac ctttagcgcc aagtatcaac aagaaccagg
    59461 tgtttccggg ccgctgaaag tcggcaactc gctggtagat gcgtttactt tgcaatatta
    59521 cgaaggtttt ccgatggatc aggtggcctg gggagaaatc aaatctgacc agcagtggaa
    59581 ggtgttgtcg aagcttaaaa acggctacca ggacagcctg tttacctcac cggaagtggc
    59641 gcgcaatgtt gcgaaaccgc tggtcagtta tatcgacaaa gctctggtca ccgatcgcac
    59701 cagcgcaccg aaaattacag tgttggttgg gcacgactcc aacattgcct ctctgttaac
    59761 ggcgctggat ttcaaaccgt atcagttgca tgaccagaac gaacgcacgc cgattggcgg
    59821 caaaatcgtt ttccagcgtt ggcatgacag caaagccaat cgcgatttga tgaaaattga
    59881 atatgtgtat cagagtgcgg aacagttacg taatgccgat gcgttaaccc tgcaggcacc
    59941 tgctcagcgt gtgacgctgg aattaagcgg ttgcccgata gatgccaacg gtttctgccc
    60001 aatggataag tttgatagcg tgttgaatga ggcggtgaaa taacagaaaa ctcccccgcg
    60061 agaagtgggg gagtcgctgg ttaaacgttt ttacgttcga tggtctgttc gccccaaaaa
    60121 agcgaatctt tatcggtctt agcaaaggct ttgactaaca cttcatcgct accttcttcc
    60181 caaatctttt ccgccatttt ttcgtcgtac ccggcgactt cgaaaatggc ctcggctatt
    60241 tccggcgacg tattgcgcag agatgcccat tcaccgacgt gatgagcttt cgcttcttga
    60301 gttggcatgc gtatcctcct gttgaagatt agccgttaag tttaactgcc agacctgcga
    60361 catattcccc ttgataacga gcaatagaca gttcttcctg gcttggctgg cgtgagccgt
    60421 caccgcctgc gatggtggtt gcgccgtacg gcgtaccgcc gcgaacctgt gaaacgtcaa
    60481 ataattcctg cgctgcgtag ccaatgggga caattaccat gccgtgatgc gcaagggtcg
    60541 tccaggttga agtaatagtt tgttcctgac cgccgccagt accggtggaa ctaaagacgc
    60601 tcgccagttt tccgtatagt gcgccggaag cccacaggcc gcccgtctgg tcgaggaagg
    60661 tacgcatttg accggacatg ttgccaaagc gggtaggtgt accaaaaata atggcgtcgt
    60721 aatcggccag ttcttgcggg gttgcaaccg gtgcagtttg cgttttaccg cctgcttttt
    60781 caaataattg cggcggcatg gtttccggta cacgcttaac gacaacttct gcgccatcga
    60841 ctttgcttgc accctcagcg actgcgcgtg ccatcgtttc aatatgtccg tacatggaat
    60901 aataaagcac cagaacttta gccatttcta accactcctc gtgttatctc tattccgtag
    60961 cgattcgcta ccacttattt aaagataaga cgtccttttc agagtgcaaa tttcacaacc
    61021 acttatttga tttataacaa ctttcacaag cacgtaattt tgtcgcaaaa tgacacattt
    61081 ttatctcatc gcgttttttt aatcataaga gcggcttatg gataattatt tgagatgata
    61141 tctattctcg ctaagaagct gttgcaggat attaccaaac gcgggtctgc ccgcgtcagt
    61201 tcactaagct tagtcccacg tagcgaaaat atggcagccg ccatacgccg cgttaattct
    61261 atgcaatatg atgtctatac ccagacggag gtcagtaatg gcaaaccatc gaggcggttc
    61321 cggcaatttt gcagaagacc gcgaaagagc atcagaagca ggtaaaaaag gtggacagca
    61381 cagcgggggt aatttcaaga atgacccgca gcgcgcatct gaagcaggta aaaaaggtgg
    61441 caagagcagt cacggcaaaa gcgacaacta gccgggctaa tcaatgacga atgcattttt
    61501 ttcttaggct cgtcaaaaag tcatcaccgc cggtaatccc ggcggttttc tgtttatggc
    61561 tcctgatgaa caacttctgg cggtggaacg tcaaccaact ttctgcttaa caacgcattg
    61621 agtaaaatcg cgccaaaggt tgctgtacca atccctccca acgtaaaacc gcccagcgtg
    61681 agagcaaaat cacccgcgcc cagcactaag gttactgcga ccataatcaa attaccgttc
    61741 tggcttaaat cgacacggtt ttgtacccat atccttgcgc ctgcgacggc aatcagcccg
    61801 aacacaacaa ttgatgcacc accaatcacc gcggccggaa tggtatgaat cagcgcgcca
    61861 aatttcggtg aaaagcccaa cagtatggcg atgacggcag cagcgacaaa caccagcgtc
    61921 gagtagactt ttgtcacggc cattacaccg atattctcgg cgtaggtggt tacgccgctg
    61981 ccaccgacag aaccagaaag catcgtcgcc agcccatcac cgacgaatgc ccgccccata
    62041 tacgggtcca tattgcgtcc ggtcatcccg gcgaccgcct tgagatgacc taagttttcc
    62101 gccaccagaa tcaccgccac gggcgcaatc agcatcattg cctgactatt aaaagcagga
    62161 gtggaaaaat gtggcaaacc gaaccaggca gcatggctga cgagagtaaa atcgacggct
    62221 tttcccagcc ctaaaaggtt ggtcatcacg ccatacagca gacaggcgac aattaaacct
    62281 acgagaatca ataaccgctg gatcatgcca cgggtaaaca ccgccaccag cccaatacac
    62341 agcaccgtca ttaccgccat ccagctatca aaggccgaag ccgatacact tttcactgcg
    62401 ataggcgcta agttcaggcc aatcgccatc accaccgcac ccgtcaccac cggcggcatt
    62461 agtcgttcaa tccagcgtgt accaattttc atcaccacca ggccaatgac ggtataaacc
    62521 agcccacagg cgataatccc gcccagcgca atgctgatat tcgggttaat gccctgaccg
    62581 ttaaagcccg tcgcggcaat caccacgccg acaaaagccg cgctggaacc gagataactg
    62641 gggacgcgcc cgccggtaat aaagaaaaac agtaacgtgc cgatccccga cattaaaatg
    62701 gaaagattgg gatccagccc catcagaatc ggcattaaca ccgtcgcgcc aaacatcgcc
    62761 accgcgtgtt gaacgcccat tactgccgtc tgagcaaacg gcaatcgttc atccggcgcg
    62821 accacgccgc tctctgtaga ggtcgatttt aactgccagt gaggaaaacc gaacattgcc
    62881 atcagctgtc tccttaagga ggttaacaag cagggcgcat cagcgcgtga taactgcgat
    62941 cgaaccacac cagcccgtag ggtgtggtgt gacgatgaat cgcttcgatg gcgcaaaaca
    63001 gaatgtcgtg ggtgccgacg ctcaccacct ggctgatacg gcagtcaaac gaaaccagag
    63061 cctcttccag ttgcgggcat ccggtcaccc ccgtctgcca gcgggcggcg gcaaagcggt
    63121 gttccatggg cgttttgccg ccaaaaaggt ttgaaagcgg ctcctgcccg gcgctcagtg
    63181 tatttacaca cagcgttcga ttttcattga ataccggcca gacggacgcc ccacgattca
    63241 ggcacaccag taatgtgggc ggcgtgtcgg tcacgctgca gacggcgctg gcggtgaacc
    63301 cggcgcgccc ggctggaccg tccgtggtga tgatattgac cgccgcgccc atacaggaca
    63361 tcgcatcgcg aaaggtttgt tgatcaacta tgttcatagt ttgctcctta caacagcccg
    63421 caggcttctt caaaggacag acgtggcagg cgcgcaaaaa gtttgctgct atcgccatag
    63481 ccgatattaa tcagcagatt gctcttcagc gtgctgcccg caaaaaaggc gtcgtcaacg
    63541 tgttgacggt caaaacccga catcgggccg gtatccagtc ccagcgcacg gcaagcgacg
    63601 atcagatagg ccgcctgcat ggaactgttg cgaaacgctg tttcttcggc aagttgtggg
    63661 ctggaggtaa accaactgcg ggcatcgccg tgaggaaaca gttgcggtaa ccgttcataa
    63721 aattcactgt cccaggcgac gatagcggtg acgggcgcgg tcagggtttt ttgcagattg
    63781 ccgctggaaa gtgccgggcg cagacgttct tttccttctg ccgtgcggat aaacacgatc
    63841 cgtgccggag aacagttagc tgatgtcggc ccccatttca tcagggcata aatctcccgt
    63901 aacgtctcat cgctgacggg tgtctcccgc cagccgttgt gagtgcgggc atcggtgaac
    63961 aaggtgctaa gcgcacctgg gctaacggct tcgttcatag caattcctta cagggcggct
    64021 tcacggtgat gtaacaggct ggcaagcccg ttgagtaaca gagcattaaa cgtttcggga
    64081 tcggtcacgt tgcaggcgtg tccgccatag ggcatcacca ttttctggct atcgggcagg
    64141 gcggcatgaa gttcactgga acatgctgtt ggcaccagca gatcatcact ggcgcagatg
    64201 atttgcaccg ggcagcggat gcgatccgca tggtgactaa agtcagcgcg tttgagggcg
    64261 ttaagtcgac gcagtaaatt atttttgccc tgaaaatgcg ccagtgccag cgcgtcttct
    64321 gcctccaggc gaggtacgcg ggccgccatc cagtcggcgg gatagaggaa caacggctgc
    64381 gcttccaccc atgcctgcgc gccgccgcta tacagtaatc gttcgcgaac ctgaaaacag
    64441 cggcgcgtat gggcgtttat tcgtagccag ccgttaacgc tgatcagcac agttaccgac
    64501 gcgggataat ccagcgccag ctgcattccc accagcgcac cgagcgcatg gccgaccacc
    64561 gcgtaatgct caatccctgc ggctaccagc gcctgatgca gttccgctgc catctgggcg
    64621 atactgtaat cttctgccag tgtgtcggga ttattgccgg tgccgcgctg gtcgtaacag
    64681 actacctgat actcctgctc cagcaccgcc agttgcggta accagtaact gccgctaccg
    64741 ccaagacccg aaatcaacac cactacgggc gcatcagcat aagggggagg tgagagtgaa
    64801 agtttcatcg cggcctcact tggcgatatg cgcaattgtg gcgatttcca ccagcgcgtc
    64861 aggttttacc agtccgcact gaatgcagaa tcgcgccggt ttatcacccg gaaaaaactc
    64921 ggcgtagatt tcgttaatcg cggcgtaatt tttccagtcg gtaataaaga tgctgttgaa
    64981 ggtcacatcc gccatcgtgc cacccgccgt ctcgatcacc ttgcggatag tttccagaac
    65041 gtggcgggtt tgcgcctttg ggtcatcggc aaacagcacg ttgttatgtt gatcaaaagc
    65101 cagcgtaccg gagacataca ccacgccatc agccagcgtg ccgggaacga agggggccag
    65161 cggtgcgctg ctgccagcgg gaataattac ggattttggc atcgttaaac tccttaagcg
    65221 atacgagcaa aggatgtggg agaaagcgcg tcgcagaacg tttcgacgtc gctgacccag
    65281 ccaaaaaagg tttcgatatt gaacaacgcg gctttctgtg caaattccgg ccccgcctgg
    65341 tgtgttgcgt cttccagcac cacgccgaaa tactccagaa aaaagccatc gcgtagcgtc
    65401 gattcgacgc agacgttggt agcgataccg gtgaaaacca gatggcgtat tccacggctg
    65461 cgcaaaatgc tgtccagcgg tgtattgaag aaaccgctgt agcgcggctt cggcagcaca
    65521 atatcgccag gctgcggcac cagttcatcc accagttgat aatcccagga gcctttcgcc
    65581 agcaatttcc cctgcagctg cggctgctta cgcatggttt tcagggcgtt cgatttatga
    65641 aaattcggtg agccgggtcc gccagcttcg acatactgtt catcccagcc attttgaaac
    65701 cagatgatca gcatccctgc cgctcgcgct gcggtcacgg cggtttgaat gttggcaatg
    65761 accgggcgag tggttgagac atcaaacccg gcgagatcta agtagccgcc tggcgtggca
    65821 taagcgtttt gcatatccac cacgatcagc gcactttgct gcggatcgaa ggtaatggct
    65881 tccggtcgag cggttaaggt tgtcatcatg ccacctcctg agtcagcgca gggagatggg
    65941 cgcggcactg catcagtggt tgaatgcgct cgccgaaggt ttcgattccc gacagaaaat
    66001 catcgaaggt caacagcacg ccttcggcac caggcacgct tgcgacttca tctaacatgc
    66061 gcgcgacact ggcgtaagaa ccgactaatg tccccatatt gatgtttacc gccgaagtgg
    66121 gatcggccat ctgacggacg ttggtgtcag taccggagcg ggtatctttc tgactttgtt
    66181 cggttagcca gcttaacgcc tcttcatccg cgcccgcttt gtagtgttcc catttggcgc
    66241 gagcggcatc gtcggtttca tcggcaatca ccataaacaa cacataagag ccaacgtcgc
    66301 gcccggtttg ctctgcggcc tgtttcatcc gcacagcggt cggggcgaaa gccgtgggtg
    66361 tatttacgcc tttgccgaaa cagaagttga aatcggcata ctgagcggag aacgccatgc
    66421 cagcgtcgct ttgcccggcg cagatcactt tcatggggac actcggttgc ggactgacgc
    66481 gacaatcatt catggtgaaa aaatcgcctt taaaatcgct tttccccgtg ccccacaggt
    66541 cgcgcagcac ctgaacatat tcggtgagat agtcgtaacg acgggagaaa tagtcatcgc
    66601 caggccagat acccatctgc tcatactcgg gcttttgcca gccagtcacg aggttgacgc
    66661 caaaacgccc gccagagatg gagtcgatgg ttgcggccat acgggcgacg attgccggag
    66721 gtaacgttaa ggtggcagca gtggcgtaaa tctgaatgcg cgaggtcacg gccgccagcc
    66781 ccgccatcaa ggtgaacgac tcaaggttgt gatcccagaa ctcagttttg ccgccaaagc
    66841 cacgcagttt gatcatcgac agggcgaaat cgaaatggta gtgctccgct ttttgcacaa
    66901 tggctttatt cagttcaaag gtcggcatgt actgcggcgc gtgggtcgaa atgagccagc
    66961 cgttgttgcc aataggtacg aatacgccaa ttttcatcat caacctctct tcgtctcgta
    67021 aagtgaaagt cagacggggc gctgcatcct gcatatcctt ttcagccgcg tattggcttg
    67081 tttgcaaagc ggatgccagt ttttaaaaag ttaatgttgt taatatgtta acaacgcgtt
    67141 atctaaaata tctggtaaaa agtggactaa acggtcaaaa cagttgcaca taaaacatgc
    67201 atctgtgcgc gatgagagtg cagaaggtcg aggccgggcg ggggttttgc tatcctgttg
    67261 ccaatctaca agaggggaga gcgcatgacg caaggcgcag tgaaaacaac gggtaaacgt
    67321 tcgcgcgcag taagcgcgaa gaaaaaagcg attcttagcg cagcactgga cactttttca
    67381 caattcggtt ttcacggcac aaggctggag cagatcgcag agttggcggg tgtttcaaaa
    67441 accaatctgc tgtattactt tccgtcaaaa gaggcgctgt atattgccgt gctgcggcag
    67501 attctcgata tctggctggc accgttaaaa gcgtttcgag aagatttcgc cccgctggcg
    67561 gcgatcaaag agtacatccg tctgaagctg gaagtctccc gcgattatcc gcaggcttcg
    67621 cgcctgttct gtatggagat gctggcaggc gcgccgctgt taatggatga actgacgggc
    67681 gatttgaagg cattaattga tgagaaatcg gcgctgattg ccggttgggt caaaagcggc
    67741 aaactcgcgc cgattgatcc gcagcatttg atttttatga tttgggcttc cactcaacat
    67801 tacgccgatt ttgcccctca ggtggaggcg gtgacaggcg cgacgttgcg cgatgaggta
    67861 tttttcaatc aaacggttga aaacgtgcag cggattatta ttgaggggat tcgaccacgt
    67921 taaagatgcc ggaggaggtt gtaacatcct ccggctacct gtttaaccga tcgtcattaa
    67981 actggcgtta ccgccagcgg cagcggtatt cacactcagc gaacgttcga tatacagccg
    68041 ttccagaagg atattgcttt cgccacgggc aaaaccctgc accgaaacaa ttgcgccatc
    68101 ccgcgcagca acggcttcac acaatgcgcg aagctgatcc gaatcaccgt ggaagatcac
    68161 cgcatcaaag ggttgagcgg ttatattttc cgctttcgcc agttgaatac gttcgctgac
    68221 tgccgatggc aatgccttta ctagctgacg atgcagcgcg tcatccggcc acagtacctg
    68281 gctgcccacc gccagcacgg cggcgagctg agtcagcgca tcctgctcat catcggcaat
    68341 acacaacacg cgctcacgcg gcagcagcgt ccaggtgttg cgttcacccg tcggccccgg
    68401 cagcaatcgt tgtgttcctg cctgcgccag ctcgccatat tgcgtacata acgcctgcaa
    68461 ttctggacga tttgctgccc attcccgcag tgcatttagc ggctgagtca atgcggcttt
    68521 caactgtgca tcgaccggat actctgcatc ctgacgcgcg agcgtcactg ccagcgcact
    68581 ttccgggcga ttcgccagca gacggtagag atagagcgga ccgcctgctt tcggcccggt
    68641 accggacaac ccttcgccgc cgaatggctg cacacccacc actgcgccca ccatattacg
    68701 gttaacatac aggttaccaa catgggccga gccagtgacc tgggcgatgg tttcatcaat
    68761 acgcgtatgg acaccaagcg tcagaccata gccggaagcg ttaatctgct cgatcagctc
    68821 tggtagctgg ttacggttgt aacgcaccac atgcagcacc ggaccaaaga cctctttttg
    68881 caattcggca aagtcatcca gttcgatcag cgtcggggcg acaaaggtgc cgctttgcca
    68941 ttcacgggca tcttcgctgt tttcccgcac cgcctggaac accggacggc ctttgctacg
    69001 catggtctga atatggcgct caatattggc tttcgcttcg ctatcaatca ccggaccgat
    69061 atcggtggtc aggcgacccg gattacccat ccggcattcg gccattgcgc cgcgcagcat
    69121 tttcaacgtg tggtcggcaa tctcatcttg caggcacagc acgcgcagcg ccgaacaacg
    69181 ctgacccgca ctgtcgaacg ccgaggccag tacatccacg acgacctgtt cggtcagtgc
    69241 tgaagaatcg acaatcatcg cgttcatgcc gccggtttca gcgatgagcg gaatagggcg
    69301 accctgagcg tccaggcggc tggcgatatt gcgctgcagt aacgtagcga cttcggttga
    69361 accggtaaac atcaccccgc gtacgcgatc atcacccgtc agttgcgcgc ccacggtttc
    69421 cccctgacct ggcagcaact gcaccacgcc tggcggtaca cccgcttcca gcaaaatggc
    69481 gatcccttgc gcggcaatca gcggcgtttg ttctgccggt tttgccagca cgctgttacc
    69541 tgccgccagt gcggcagcaa tctgcccggt gaaaatagcc agcgggaagt tccacggact
    69601 gatacacacc acaggcccta atggacggtg ggtttcgtta gcgaaatcat cccgcacctg
    69661 tccggcatag tagtggagaa aatcgaccgc ttcgcgcact tcggcaatgg cgttactgaa
    69721 ggtttttccg gcttcacgca ccagaatacc aatcagttgc tgcatctggc tttccattag
    69781 cacggcagcg cgatgcaaaa tcgccgcgcg ttcaaccgga ggagtggcaa accagattgg
    69841 cgcgttatta accgcacttt ccagcgcctg ttctacctca cgcggcgtgg cttcacgcac
    69901 aaagcccaca atatctttcg gttccgcagg gttaataacg ggcgacatct cacctgccgc
    69961 taccggttgt tccagcattg gcaaggcctg ccatttttgc agtgcactat tgagcagggc
    70021 agaagagagc gaggccagcc ggtgttcgtt agcgagatcc agccctgccg agttgtcgcg
    70081 cccgtgaccg taaagatcgc gcggcagggg aattttcgga tgcggtaatc cagtttgccc
    70141 ttcctgttgc gccagttttt ctacagcagt gaccggatcg gcgaccagtt catccagtgg
    70201 caaagaggtg tcggcaatac ggttaacaaa cgaggtgtta gcaccgtttt ccagcaggcg
    70261 acgcaccaga tacgccaaca gcgtttcatg ggtaccgacc ggagcataaa tacgacacgg
    70321 acggttaagt ttgccgtcgg caactttccc ggtgacctgc tcatacagtg gctcgcccat
    70381 accatgcagg cactggaact cgtactgacc cggatagtag ttctgacctg ccagttgata
    70441 aatcgctgcc agcgtatggg cgttgtgcgt cgcgaactgc gggtagatta gattcggcac
    70501 cgccagcagc tttttcgcac aggcgagata tgaaacgtcg gtatacacct tgcgggtata
    70561 aaccggatag ccttcaaggc cgtccatctg cgcacgcttg atttcactat cccagtacgc
    70621 gcctttcacc aggcgaatca tcagacggcg acggctgcgg gtggcgagat caatcaggta
    70681 atcaatcacc agtgggcagc gtttttgata agcctgaata acaaaaccga taccgttcca
    70741 gcctgccagc tcaggctcga aacagagttt ttccagcaga tcgagggaga tctccagacg
    70801 atcggcctct tcggcgtcaa tgttgatacc aatatcgtac tgacgcgcca gcagggtgag
    70861 tgatttcaga cgtgggtaaa gctcttccat tacccggtca tactgggcgc ggctgtaacg
    70921 cggatgcagc gccgacagct tgattgaaat gcccggacct tcatagatgc cacgaccatt
    70981 ggacgcttta ccaatggcgt gaatcgcctg ctgataggaa accatatacg cctgtgcatc
    71041 tgcggcagtc agtgcagctt cgccaagcat atcgtaagag taacggaaac ctttctcttc
    71101 cagcttgcgg gcattggcta acgcttctgc aatggtttca cccgtgacga actgctcgcc
    71161 catcaggcgc atcgccatat ccacaccttt gcggatcagc ggttcaccgc tcttaccgat
    71221 aatgcggttc agcgagcggg agaggctggc ttcgttatgg gtggaaacca gtttgccggt
    71281 aaacagcagc ccccaggtag cggcattaac aaacagggac gggctacgac caatgtgtga
    71341 ctgccagtta ccgttgctga ttttgtcgcg aattaatgca tcccgggtgg ctttgtcggg
    71401 aatacgcaac aacgcttctg ccagacacat cagcgccaca ccttcctgcg atgacagcga
    71461 aaattcctgc aataacccct gaaccatgcc tgcgcgacca ctggcatttt tttgattacg
    71521 cagtttatcg gccagttgat acgccagttt gtgcgcctgt tcagcaactg gctgcggcag
    71581 gcgggcttgt tccagcagca tagaaaccgc ttcggtttcc gggcggcgat aggccgctgt
    71641 gatcgcggcg cgggaaaccg actggggcaa tatttgctcg gcaaagtcga ggaatggctg
    71701 gtgtggttcc tctgccggag tcggtgcttc atcgctctca ttggccgcgc cagaaagcag
    71761 cgcaggtagc tccggcagag tatcgctgtt ttccagttgt tcaagataag aaaaaatcgc
    71821 ctgcttaatt aaccagtgtg gtgtgcgatc gatacgtgtc gcggcagact taatacgctc
    71881 acgcgtcgcg tcgtccagct taacccccat ggtggtggtt cccatgccat tactcctgtt
    71941 gttctgaaag gtgcaactta acgttatcgt gaaatatcca tgatgttgca actttgtgca
    72001 accatgttaa atgtgacctg cgtagcaagc ttaaaaatga atgaaatgtt aataaaagaa
    72061 atcgatatga cagggattaa aaaataactc agactttttc tctgcggcag ttaacatttt
    72121 tgaaaggtgc aaccgcaaaa aatgtgagag agtgcaacct gatgaaaaat agtgtcgctg
    72181 agcactaaaa tttaatgtaa atggtgtgtt aaatcgattg tgaataacca gcgcttccgg
    72241 caggatacgg tcgccctggt aaaacataaa ctctgttacc ccgttccggt ggcagatata
    72301 acggcaagtt tcgacattgc cgataataat tttttggaga ctttagatgg ctattagcac
    72361 accgatgttg gtgacatttt gtgtctatat ctttggcatg atattgattg ggtttatcgc
    72421 ttggcgatca acgaaaaact ttgacgacta tattctgggc ggtcgtagtc ttgggccatt
    72481 cgtgacggca ttatcggcgg gtgcgtcgga tatgagcggc tggctgttaa tggggttgcc
    72541 gggcgcggtt tttctttccg ggatttccga aagctggatc gccattggcc tgacattagg
    72601 cgcgtggatc aactggaagc tggtggccgg gcggttgcgt gtgcataccg aatacaacaa
    72661 taacgcctta acattgccgg attatttcac cgggcgcttt gaagataaaa gccgcatttt
    72721 gcgcattatc tctgcgctgg ttattttgct gttcttcacc atttattgcg cttcgggcat
    72781 tgtggcaggc gcgcgtctgt ttgaaagtac ctttggcatg agctacgaaa cggctctgtg
    72841 ggcgggcgct gcggcgacga tcctttacac ctttattggc ggtttcctcg cggtgagctg
    72901 gactgacact gtacaggcca gcctgatgat ttttgccctg atcctgacgc cggttatcgt
    72961 cattatcagt gtcggtggct ttggtgactc gctggaagtg atcaaacaaa agagcatcga
    73021 aaacgtggat atgctcaaag ggctgaactt tgttgccatt atctcattga tgggttgggg
    73081 gctgggttac ttcggtcagc ctcatattct ggcgcgtttt atggcggcgg attctcacca
    73141 cagcattgtc catgcgcgtc gtatcagtat gacctggatg atcctctgcc tggcaggggc
    73201 ggtggctgtc ggcttctttg ggattgctta ctttaatgag cacccggcgg tagcaggtgc
    73261 ggtaaaccag aacgctgaac gtgtgtttat cgaactggcg caaattctgt ttaacccgtg
    73321 gattgccggg attctgctgt cggcaattct ggcggcggta atgtcaacgt taagctgcca
    73381 gctgctggtg tgctccagtg cgattaccga agatttatac aaagcgtttc tgcgtaaaca
    73441 tgccagccag aaagagctgg tgtgggtagg gcgtgtgatg gtgctggtgg tggcgctggt
    73501 ggcgattgcg ctggcggcaa acccggaaaa ccgcgtgctg ggcttagtga gctacgcgtg
    73561 ggcaggcttt ggcgcggcgt ttggtccggt ggtgctgttc tcggtgatgt ggtcacgcat
    73621 gacgcgcaac ggtgcgctgg cggggatgat catcggtgcg ctgacggtta tcgtctggaa
    73681 gcagttcggc tggctgggac tctacgaaat tattccgggc tttatcttcg gcagtattgg
    73741 gattgtagtg tttagtttgc tgggtaaagc gccgtcagcg gcgatgcaaa aacgctttgc
    73801 cgaggccgat gcgcactatc attcggctcc gccgtcacgg ttgcaggaaa gctaagggac
    73861 ttagcctgcg gcggttttgt ttggcttcag cagcgggttg cgctccctta atgtgcctcg
    73921 ccatataaat tgaatggtgc agggagcgcg cagggggcgg ccaatcgccg ccgccccctg
    73981 ctgtcccggc cttcggggaa cgcttcagcg attttgacgc caccaacacc cgagctgtta
    74041 ttatgttccg ggcaaaaagt tagatttgat aatcgcggat ggacgaaatt gcttgataca
    74101 cccgcttatc agttttacat ggaagctctg atgcattgag tctggacagt tttgtcggct
    74161 ggatacggcg tttacgcggc atccggcaag aacacatggt tctttgcaaa caatcccatc
    74221 tttctaccct ggaataatcg tttatatccc ttggcattac ctctctttgt ttacattcca
    74281 acatcatttt ataaacattc cgcttgtatt tttctttgcc gtaatgataa tcgctatcac
    74341 tgcgatttac ttttctttgc atagattgac tcagaaaaac gtttaagggt gggtggcatg
    74401 tttgttccgt ttctcattat gttgcgcgaa ggacttgaag ccgcgctgat tgtcagtttg
    74461 attgccagct atcttaagcg tacccagcga ggccgatgga ttggtgtgat gtggattggc
    74521 gtgttgcttg ccgctgcgtt gtgcctgggc ctgggtatct tcattaacga aaccaccggc
    74581 gaatttccgc aaaaagaaca ggaactgttt gaaggtatcg tggcggtgat cgccgtggtg
    74641 atccttacct ggatggtttt ctggatgcgc aaagtgtcgc gcaacgtcaa agtgcaactg
    74701 gaacaggcag tcgatagcgc attgcagcgt ggaaatcatc atggctgggc gctggtgatg
    74761 atggtctttt ttgccgttgc aagggaaggg ctggagtcgg tctttttcct gctggcggca
    74821 tttcaacaag atgtcgggat ctggccgccg ctgggtgcaa tgctcggtct tgctactgcc
    74881 gtggtgctcg gcttcctgct ctactggggc ggtattcgcc tcaatcttgg tgcatttttt
    74941 aaatggacca gcctgtttat tctcttcgtc gccgcagggc tggcggctgg tgccattcgc
    75001 gcatttcatg aagccggatt gtggaaccac tttcaggaaa tcgccttcga tatgagcgcg
    75061 gtcctctcaa ctcactcgct gtttggcacg ctgatggaag ggatttttgg ctatcaggaa
    75121 gcaccgagcg tcagcgaagt cgccgtctgg tttatttatc tcatcccggc gctggtggca
    75181 tttgccctgc caccacgcgc aggggcgaca gcgtctcgct ccgcgtagca aatacgacgc
    75241 aaactcttgc ttagttacaa catactttaa agggatagtc tcgtcatgac cattaacttc
    75301 cgccgtaacg cattgcagtt gagcgtggct gcgctgttct cttctgcttt tatggctaac
    75361 gccgctaatg tgccgcaggt caaggtgacc gtgaccgata agcagtgcga accgatgacc
    75421 attacggtta acgccgggaa aacacagttc attattcaga accacagcca gaaggcgctg
    75481 gagtgggaga tcctcaaagg cgtgatggtg gtggaagagc gggaaaatat cgcccctggc
    75541 tttagccaga aaatgacggc gaatttacag cctggcgaat acgatatgac ctgcggtctg
    75601 ctgactaacc cgaaagggaa gttgatcgtc aaaggtgagg caacggcgga tgcggcgcaa
    75661 agtgatgcgc tgttaagtct tggtggtgca attactgcat ataaagcgta tgtcatggcg
    75721 gaaaccacgc agctggtgac cgacacgaaa gcctttactg acgctattaa agcaggcgat
    75781 atcgaaaaag cgaaagcact gtatgcgccg acgcgccagc actatgagcg catcgaaccg
    75841 attgctgaac tgttctccga tctggatggc agcattgacg cccgtgaaga tgattacgag
    75901 caaaaagccg ccgatccgaa attcaccggt ttccaccgtc tggaaaaagc attgtttggc
    75961 gacaacacca ccaaaggcat ggatcagtac gctgaccagc tttataccga tgtggtcgat
    76021 ttgcaaaaac gcatcagtga actggctttc ccaccttcaa aagtggtcgg cggtgcagcc
    76081 ggactgattg aggaagtggc agccagcaaa atcagcggtg aagaagatcg ctacagccac
    76141 accgatctgt gggatttcca ggctaacgtt gaaggctcgc agaaaattgt cgatctgctg
    76201 cgtccacaac tgcaaaaagc caacccggaa ctgttggcaa aagtcgatgc caactttaaa
    76261 aaggtcgata ccattctggc gaaataccgt actaaagacg gttttgaaac ctacgacaaa
    76321 ttgaccgatg ccgaccggaa tgcactgaaa ggaccgatta ctgcgctggc ggaagatctg
    76381 gcgcaacttc gcggtgtgct gggattggat taagcattat gcagtatgaa gataaaaacg
    76441 gcgtgaatga accgtcacgc cgacgtttac tgaaaggcat tggtgcgctg gcgctggcgg
    76501 gaagttgtcc ggtcgctcat gcacaaaaaa cgcaaagtgc gccgggtacg ctttcaccgg
    76561 atgcacgcaa tgagaaacag ccgttttatg gtgagcatca ggcagggatc ctgacgccac
    76621 aacaggccgc aatgatgctg gtggcgtttg atgtgcttgc cagcgataaa cccgatcttg
    76681 aacggttgtt tcgcttgttg actcagcgtt ttgcttttct gactcagggc ggagcggcac
    76741 cagaaacgcc aaatccgcgc ctgccaccac tcgattccgg cattcttggc ggctacattg
    76801 cgcccgataa tctcaccatc acgttatcgg tgggtcactc attgtttgat gagcgctttg
    76861 gccttgcgcc acagatgccg aaaaagttgc agaagatgac gcgttttccc aacgactcgc
    76921 tggatgcggc gttatgtcat ggtgatgtgt tgctacaaat ttgcgccaac acccaggaca
    76981 cggtgatcca tgcgctgcgc gatatcatca aacatacgcc agatttgctc agcgtgcgct
    77041 ggaagcggga agggtttatt tccgatcacg cggcgcgtag taaaggcaaa gagacgccga
    77101 ttaatttgct gggtttcaaa gacggcactg ccaatcccga tagccagaat gataagttga
    77161 tgcaaaaagt ggtgtgggta acggcagatc agcaggagcc agcgtggaca atcggtggca
    77221 gctatcaggc agtacgcttg attcagtttc gagtggaatt ttgggacaga actccgctga
    77281 aagaacagca gacgattttt ggtcgtgata agcaaaccgg tgcgccgctg ggaatgcagc
    77341 atgagcatga tgttcccgat tacgccagcg atccggaagg gaaggtgatt gcgctggaca
    77401 gccatatccg gctggcgaat ccccgcacgg cggagagtga gtccagcctg atgctgcgtc
    77461 gtggctacag ttattcactg ggcgtcacca actccgggca actggatatg gggctactgt
    77521 ttgtctgtta ccaacacgat ctggaaaaag gcttcctgac agtacaaaaa aggctcaatg
    77581 gcgaagcgct ggaggaatac gttaaaccta tcggcggcgg ttattttttt gcgctgccgg
    77641 gagtgaagga cgcgaacgat tatctcggaa gcgcgttatt gcgggtttaa tgtttttaga
    77701 cggataaggc gttcttgcgc agatgcctga tgcgatgctt gcgcgtcttc ttatgcctac
    77761 aatcggtgcg ggttcggtag gcggataagg cgttcacgcc gcatacggcg atcgtgcaca
    77821 gatgcctgat gcaaatcctg ctgaaagcac acagcttttt tcatctctgt catcactctg
    77881 tcatctttcc agtagaaact aatgtcacta aaatggtgtt ttatagttaa atataagtga
    77941 atatattgtt gcaatgaatg cgagatctgt tgtacttatt aagtagcaga ggaagttccc
    78001 ggcagtgata gtcagtcact atggagatcg cggatggtaa cgtcctgtac tggacaatgt
    78061 tttgttaagc aacgctccac cactcgcgga gatttctcct ccggtagcaa cttcgtcact
    78121 ctgtattttt ctcacttcct ctctttactc gtgttaccga cgccgcaaac ggtgtgcgta
    78181 gccgtttttc ggcattttat ccaaaagcaa actatggctt acaaggaagc caaccctctg
    78241 atgttcgtgc gcataatcgc gctgccaacg gcgcgtgtga tgaatacaaa caactcaagg
    78301 tgctctccat gggaagacaa aaagcagtga tcaaagctcg tcgtgaggca aaacgtgtgc
    78361 tgagacggga ttcgcgcagc cataagcagc gtgaagaaga atcggtcacc tcgcttgtgc
    78421 agatgagcgg cgtagaagcc attggtatgg ctcgcgacag tcgcgatacc tcgcctatcc
    78481 tcgcgcgaaa tgaagcgcaa ttgcactatc tgaaggctat tgagagtaag cagctgatat
    78541 tcgccacggg cgaagccggg tgcggaaaaa cctggatcag tgcagcaaaa gcggcagagg
    78601 ccctgataca taaggatgtc gacaggatta tcgtcacccg tccagttctg caagccgatg
    78661 aagatcttgg cttcttacct ggagatatcg cagaaaagtt tgctccctat tttcgcccgg
    78721 tctacgacgt gctggtccgg cgcttagggg cttcctttat gcagtactgc ctgcgaccgg
    78781 aaatagggaa ggtggaaatc gcgccgttcg cctatatgcg tggacgtacc tttgaaaatg
    78841 cagtcgtcat tcttgacgag gcgcagaatg tgactgccgc gcaaatgaaa atgtttttaa
    78901 cccgcctcgg ggagaacgtg acggttatcg tcaacggtga tatcacacaa tgcgatttac
    78961 ctcgcggtgt gcgctccgga ttaagtgacg cgctggaacg tttcgaagaa gatgaaatgg
    79021 tcgggatcat ccgattcggt aaagaggatt gcgtacgttc ggcactttgc caacgtacgc
    79081 tgcatgcata cagttaagtg tattatcggt gcagagcccg ggcgaaccgg gctttgtttt
    79141 gggtgtttat gcccggacta gcgctttttc tgaaacaacc atttttattt gcccctggct
    79201 ggtgaagtgt acgctcatcc tgtggctttt ttgtagttgc tgatagagct catcaggtat
    79261 tgctaagctc tctgcatatt cttgtggcgt atattggtag gctgcatgat gctgctgttt
    79321 ttgaaaacgc agcttattgt acagcgccca gacaattaac acgacggcat tcgccactgc
    79381 cagcaaaaaa tagaactgaa gtcggcttct ggcctcgctt tgccagtaat aacccgtcag
    79441 cagatccatg gcgaataaga atatgaacaa agcaaataat gtccacaaga tggttgtggc
    79501 aacataatca accagtaaac gtactggcga ttgtcgggtc gtaataatta aattgttcat
    79561 attaacctct cagaatcccg cgatcgggac ttacccaacg ggcgcgttgc tttttaggca
    79621 tcaacatgac tcgtgtaaat gataccaatg tcgttgccag gctcagcatc cagaaaataa
    79681 ccgggaacca aataatccag aaaagcgatg aagtcagatt atgctcatag cgattctcga
    79741 tcatcaggct gacaataaat tgcagtaaac ataacgtaca caataatatt ccggcagtat
    79801 gtgtcgcagc gatatgtgtc aattcaatat ttaacggtac accggcaagt tggactgcgt
    79861 aaataatgaa accgaccagg caggtaaagg cccatattgt cgtcagacag tattcaaaaa
    79921 acagtggcca cattcgaaag ttttctttgc gccatagcct tgtcatattt ttgaggaata
    79981 cttctgcacc gccctgagcc cagcgcaggc gctgtttcca cagccctttt aacgtttcag
    80041 gcattaatat ccagcacagc gcccgtggct cgtaaaaaat cgtccactga ttcaactgca
    80101 gcttccagct aatatcaata tcttcggtga tcatatcgtc actccagtaa cccacttctg
    80161 ccagggcgct gcgacgaaat gcggcaataa caccggaaac ggtaaatacg tttccataga
    80221 tacgctgggt tcgcttgatc aaaccaataa ttgaggaata ctcgccaacc tgaattttac
    80281 ccaccagggt agaacgtgtt cgaatacgag gattaccggt tacggcaccc acacgcgggt
    80341 tgtacaacat cggttccaca atatatgccg ccgcatcgcg gtctaataac gcatcgccat
    80401 caatgcacac cagatattca cttttcgccg cggcagctcc ggttttaagc gcaatggctt
    80461 tcccctggtt ttgcgccaga tgaatgaccc gcaaatgggg aatttgtgca gccatgcgat
    80521 ccaggatggc acgggttcta tctgttgaac cgtcatttac ggcaataact tcaatgttct
    80581 cataacgctg tgctaaagcg gcgtgtatgg tttcctctac gtttttctcc tcattaaaac
    80641 agggaatgat aatggagata gacggattat ctttcaactg gggagctggt gcgttttctc
    80701 cccacggcca gtggcgttca cgatagaccc agaaatagac gccgccaaca atccacataa
    80761 tggacataaa aaacggccag aagaaaacga acctcatcat cagttcacca gagtgaaagt
    80821 acgctacgca tagggggatg catagcacca gacataatat aaagaacgat acgatgcgat
    80881 taatcatttt tcggatacca ggctgttgaa aactcaggac gaataacgtc tatttcaggt
    80941 tggtcatgca gaaaattgtc gggataataa ccatagtttt tcactccatt cagttgtaat
    81001 aggctcatcc agtgagcgag ttgttgcgaa gaaatagcct gatgctgacc atttttctgc
    81061 cagttttgtg cctgtaattc taaaatagat ttgtctttag cctgagggat gtttttaatt
    81121 tgattggtca attgtattaa ccattggtca gccgattttt ctgcgacacc ttccatataa
    81181 ggcatagcca taatagcggt ccagtcatag ctttttagga aatcagcata attctgtgca
    81241 aaccaggctt cactttcagg ttgtattacc ggaagtgcaa aaatatttcg tgcagtttta
    81301 acatgtggac cgcgaatggc ttttacgcgc gcactaagtt ctaaagtgaa gtcagttaac
    81361 gcacgacttt taaagcgggt ccactgttta aattgctccg ggttttgtcg aatttcgctc
    81421 agactcccgc taaagcctgc ttgctgatag gccgtgatag ccggtgcact ggcatcttca
    81481 taatctgaaa gcaaagcatc atcgtggaac aataggccat caaaagcagc atgtccggca
    81541 agatcttcat ataacatgcc aacttgtgct ctgaccttgt catcaaaagg agagagacgg
    81601 cgatattgtt ctggatgaat ttgtgctttt ttctcccctg ttggtaaata ttttactcgc
    81661 gttaatgtgg gatctaaatc ccagcttaat accggcatcc acgcatagat gtttacacct
    81721 gagcgggtac gtaattgcca ggcaacccga ctaaaaatat ctgctttcat tggtagcaaa
    81781 cgatttggaa accagacctc tttgaccagc ccatcaccat cgggatcagc aaatgcctgc
    81841 aaatacacgg ttgatatttg catatctttc acccgctgaa ttagcacatc aatattgcga
    81901 tccatttgct ggcgattttc gtcataaacg taatcaagat cgatatgcat tatccgttgt
    81961 gatgattttt cctgtacggt aataatttgc tgggcaaact cttttaatga gggattattg
    82021 gcgattaata ctcgcggaat ggaatccaat tgtgacgaat ttgccaaacc tgattcaagg
    82081 gtgaagaaca tgtcataacc gagttttttt aattcctcta tcgctatgcc attcgcttcg
    82141 ccataaggcc aaacaaaaac gtgtggattt accttagcct ttgtacgcag gtattctgtc
    82201 atttttacag catccagacg aattctttcc cggtattctg ctgcggtttc ataccgtgcg
    82261 tggtcagtaa aatatgcacg atttacatat acaggcaata agctgccggt ggcattagcc
    82321 tgaataccgt agtgagaatt ccatgtatga gaagcgacct caacgagcgg ggaacgcgca
    82381 acttctcgca cttgttgcca cgtggcaaaa tattctcgat cgaccatctc atcgccaaat
    82441 tttacttgtt catccgctgg cgtatcgacc caactgccga cgggggccca tacagcaggc
    82501 cattggaagg cctgaagaat tgggaagacg cgggtataaa aactctggta gccgtcatca
    82561 aaagtcagca ctactgcttt ttccggtagc ggttttcctc ctcgatgtgc ttcacgaatt
    82621 tgagcaatac tgacaggttg ataaccgttc tcgcgcagcc aggcaaattg ttcacgcagt
    82681 gctgatgtcc gcactgacat aaaacgctgg tcggcagctt cgtcttcaac gttatgccat
    82741 gaaatcgcca caaaaccatt atgcggccac ggttgctcgg cgagtaaaga ctcgcgatcc
    82801 tgtggcggta taaatgatgt ttttgactgg ctaatgcacg cagtgagcat aattatactc
    82861 accagcatca ggagatattt atttccatta cgtaacatat ttatccttaa aatctgaatg
    82921 tcatatcgaa ttcaacgtat aagttgtgtt ctctgtcacc gtcataaggt cgtttttccc
    82981 agcgtagcgt tgcgccagca tcaatcacgt cattccagct aatgcgttgc ccgtagccga
    83041 gttgggtgac gacatccgtg ccataatgtt tttgccagga ggcaccaaca cctgcgctga
    83101 agatttgctc ccagctattt tcatagcttc gccataacaa atgacttgcc tcaaatgccg
    83161 gaacaatatc gaacgttttt atagggttgt agtatggggt atcgtgttct gtattttgtt
    83221 cgtaatacag actgggtagg aaatcgacaa tcaaatatgg tgaagactag atgcgttcct
    83281 gaccatcaat tgagacttca tgacgctggt tactgtcgga aaaatcagtg aaagcccagg
    83341 agacaccgta cttacgccgc tcattttgat accagcgaac ataagcctga gcactgttgc
    83401 ctgtaacacc atttttcatt gcccgtaatg gaacgcggtg agagaggcgt tccagttgcg
    83461 aaccaatacg cctgttatca ttaaaatcat accagccaga caggcgcgcg ccgggtttat
    83521 gctcatgatt gaaaaagcgt tcagcgtact ccgcctcgag ccagatatta cgtgaccgcc
    83581 actcaacacc cgccagccag tcgcgaacaa tcccttttcc ttcgctaaat tgtccatcgg
    83641 cataaccgaa tccagcaaaa ccgcgccagt tatccttcag cggtggtgaa taaacgatgg
    83701 tggttaagtc gacatcatgt ttaccactat ccgggccttc ggcatcaatt cctgttgagc
    83761 cagcgatacg aagctctgca agattatgta catcaaccgc acgttttaat cgtacaacgc
    83821 cgggatcttg cggttcacgt tcgacaacat cgtgcgttaa gacagctgcc tgctgccatt
    83881 cttgtaacgt taatgctgtc caggcttgtt caacctccag attaatatta cgtggctcga
    83941 tcacttctgc tttttttaat tcattttctg ctgcacgagg ccaaccgcgg gcttgtaaca
    84001 cactcgcgta atcaatgcgc agtccctgat ttcctggtgc gttataagca agctctctgg
    84061 ctgtcatttc agcctgagga agatcattac tatattttgc tacggttgag agaaacgaat
    84121 gcccctgtaa ccaggtatca ttcgggatgc tcgtaggcgt gcccattaac cgaaggaaag
    84181 gcggcgaagt attaatggta tgttgggtga cagttagcgc gcccggataa ttttcactct
    84241 ccaggtggct gtaaaagaga tccgcaagtt cttcatcgga taaatccggg gcaatggtct
    84301 ccttgtgata aaagagctcg gtcattattg actgtgcttt tttcggctga tgatctttga
    84361 gataagccga tgcaacccaa tattgccccc agggcggaat aatttgcccc gtctttttta
    84421 atcgctgata gtgagaaata acgtctttat aacgatcgcg agttaataac gcgccaagat
    84481 gatcaacctg aatacgctgg tactgggcag tgtggtctgg gttatcgtgc cacagaattt
    84541 ctaatgcagc gtattgggcg agggcgcgat cggcaatggc ataacgttca ctttcactgc
    84601 gcgtaggcat aaacgacagt ctgaccagtt cggcatgaat atcagcgcga atatctggcg
    84661 ttaaattggc atcgtcaatc gcggcagcaa gttgattatt acgtaatgcc tgcacgtatt
    84721 ctgtgggata ttgttgcgta gatgcatttt caggtaatga ctctgtcatc gcccgtaatt
    84781 catcctgatg acgccccgcc agtttataga tataggcttc tgcgagtaaa ttggctttgt
    84841 ccggtgctcc agagttaagc tgcttaagtt taaccagcgc agtatcatag tgacccgcat
    84901 ctgccagggt taaaatttgt ccccgttgat aatccttatt ttgcggctcc agagagagcg
    84961 ccttttgcca cagtgtaagc gagttttgcc attgttgcag gttacgataa gcgacggcga
    85021 cagctgcata accacgcgct ggtaattgct gataacggta gcggttgtaa acggtaataa
    85081 cctgtttatc ttgcccggcc cataatgcaa tctgtaacca gtcagcaatt tggttattac
    85141 tgagtgctga tttttgtgca aaccataaca aagctggctg agtatttccc ttgcgagctt
    85201 caataatcaa tgcatcatag gcgttattaa cggcactctt cgccgcggga ctcgctgcta
    85261 aaaatgcggc agtaagaatt ttcaaagccc atttggtttt cgggcacctt tttctgctac
    85321 ttgaatacat cctgtattac tccatgtatc gccaaaatct ctctctgtat ctaattacag
    85381 gtaactgaaa agaaagatat ttttgcacct cataatccgt tattaaacgc ggaagagaga
    85441 cgtgaattgt tgatgatgag aagaagaaat gatgagcaga gtgtccatat aaaatccttt
    85501 tctcgcccga aaatccattc caatgatgag gatcttcagg aatacggcat aaatcccaat
    85561 gcctttttca aaataaatta ggattaaaat aattaaatca gtaaattccg atgcatgatt
    85621 tcggattttt cgaaagtcct gatgaaaggc tgcgctacgg cactcacgga tttaattgtt
    85681 attcctaatg tatctatgaa ttaatgtttt ataaggattt tctataacca tttataagta
    85741 ttttcaagcc tggcttgttg caaacaatgt ataaagcact taggcaataa taattacatt
    85801 cagcaactat catcatcggt attgtttgtg ggcggaattt catattgaca acagtacagt
    85861 tcttatatct attaataata gaaagggatc tacaacctac agattggtgt agctttatgg
    85921 aaaaagacta tttgagaatt agtagtactg tattagtgag cttattgttt gggcttgctt
    85981 tggttttggt gaatagttgg tttaatcagc caggcgttga ggaagttgtt ccacgatcaa
    86041 cctatctgat ggtaatgatt gctttatttt ttatcgatac tgttgcattt atttttatgc
    86101 agttgtattt catttatgac cgtaggcaat tttcaaactg tgtacttagt ttggcttttc
    86161 tgagttgttt gatttacttt gttaaaaccg tcattatcat tcagcaaatt attgaggagc
    86221 gtttgacaag cagtgttgtc caaaatgaca ttgcaatcta ttatttgttt cgtcagatga
    86281 gtttgtgcat attaatattt ctggcattgg tgaataaagt tagtgaaaac acaaaacagc
    86341 gcaatttatt ttccaaaaaa atgactttgt gcataagttt gttttttgtt tttggggggc
    86401 cgatagttgc tcacatactt tctagtcatt atgagagcta caatttacac attgccgagt
    86461 taaccaatga aaatggtcag gtggtttgga aagcctcata tgttaccata atgattttca
    86521 tgtggttaac cttgctttcg gttaatctat actttaatgg tttacgctat gatatctgga
    86581 atggagtgac ggtgattgct ttttgtgccg tactgtacaa tatctctctg ttatttatga
    86641 gcaggtatag cgtgtccact tggtatatta gtcgcactat tgaagtagtc agtaagttaa
    86701 ccgttatggt aatatttatg tgccatatat tcagtgcgct acgagtaaca aagaacattg
    86761 cacatcgcga tcccttaacc aatatattta acagaaatta tttttttaat gaactgacag
    86821 ttcaatcagc atcagcccaa aaaacgcctt attgcgtcat gattatggat atcgaccact
    86881 tcaaaaaagt caacgacacc tgggggcatc cggttggcga tcaggtgata aaaacagtgg
    86941 tgaatatcat tgggaaaagc atacgaccag atgacctttt agcgcgcgtc ggcggcgaag
    87001 agtttggcgt cttgctgacg gacatcgata ctgaacgtgc gaaagcttta gcggaaagga
    87061 ttcgggaaaa tgttgagcgt ttaactggcg ataatcctga atacgctata ccacaaaaag
    87121 tgacgattag tattggcgct gttgtgactc aggaaaatgc gttaaaccca aatgagatct
    87181 atcgactggc tgataacgca ctttacgagg cgaaagagac cgggcgtaat aaggtggttg
    87241 tgagggatgt ggtgaatttt tgtgagtcac cataaagcgg catt
//
LOCUS       DAJOWB010000006        85975 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJOWB010000006 DAJOWB010000000
VERSION     DAJOWB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 85975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.006"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            157..600
                     /gene="cmtB"
                     /locus_tag="OHO85_000470"
     CDS             157..600
                     /gene="cmtB"
                     /locus_tag="OHO85_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCQ2669899.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            628..2016
                     /gene="cmtA"
                     /locus_tag="OHO85_000471"
     CDS             628..2016
                     /gene="cmtA"
                     /locus_tag="OHO85_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCQ2669900.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            2031..3308
                     /locus_tag="OHO85_000472"
     CDS             2031..3308
                     /locus_tag="OHO85_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCQ2669901.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3305..4270
                     /gene="yggF"
                     /locus_tag="OHO85_000473"
     CDS             3305..4270
                     /gene="yggF"
                     /locus_tag="OHO85_000473"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCQ2669902.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4292..4801
                     /gene="fumE"
                     /locus_tag="OHO85_000474"
     CDS             4292..4801
                     /gene="fumE"
                     /locus_tag="OHO85_000474"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCQ2669903.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLLGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4798..5511
                     /locus_tag="OHO85_000475"
     CDS             4798..5511
                     /locus_tag="OHO85_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCQ2669904.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5483..6160)
                     /locus_tag="OHO85_000476"
     CDS             complement(5483..6160)
                     /locus_tag="OHO85_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCQ2669905.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(6154..6831)
                     /locus_tag="OHO85_000477"
     CDS             complement(6154..6831)
                     /locus_tag="OHO85_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCQ2669906.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6819..7526)
                     /locus_tag="OHO85_000478"
     CDS             complement(6819..7526)
                     /locus_tag="OHO85_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCQ2669907.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7527..8105)
                     /locus_tag="OHO85_000479"
     CDS             complement(7527..8105)
                     /locus_tag="OHO85_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCQ2669908.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8128..8559)
                     /locus_tag="OHO85_000480"
     CDS             complement(8128..8559)
                     /locus_tag="OHO85_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCQ2669909.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8931..9950
                     /gene="epd"
                     /locus_tag="OHO85_000481"
     CDS             8931..9950
                     /gene="epd"
                     /locus_tag="OHO85_000481"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCQ2669910.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            10000..11163
                     /gene="pgk"
                     /locus_tag="OHO85_000482"
     CDS             10000..11163
                     /gene="pgk"
                     /locus_tag="OHO85_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCQ2669911.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11378..12457
                     /gene="fbaA"
                     /locus_tag="OHO85_000483"
     CDS             11378..12457
                     /gene="fbaA"
                     /locus_tag="OHO85_000483"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCQ2669912.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            12815..13675
                     /gene="mscS"
                     /locus_tag="OHO85_000484"
     CDS             12815..13675
                     /gene="mscS"
                     /locus_tag="OHO85_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCQ2669913.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13814..14449
                     /gene="argO"
                     /locus_tag="OHO85_000485"
     CDS             13814..14449
                     /gene="argO"
                     /locus_tag="OHO85_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCQ2669914.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14542..15282
                     /locus_tag="OHO85_000486"
     CDS             14542..15282
                     /locus_tag="OHO85_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCQ2669915.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15449..16345
                     /locus_tag="OHO85_000487"
     CDS             15449..16345
                     /locus_tag="OHO85_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2669916.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEGRQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHKPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(16342..17820)
                     /gene="scpC"
                     /locus_tag="OHO85_000488"
     CDS             complement(16342..17820)
                     /gene="scpC"
                     /locus_tag="OHO85_000488"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HCQ2669917.1"
                     /translation="METQWTRMTANEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYQDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17844..18629)
                     /gene="scpB"
                     /locus_tag="OHO85_000489"
     CDS             complement(17844..18629)
                     /gene="scpB"
                     /locus_tag="OHO85_000489"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCQ2669918.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18640..19635)
                     /gene="meaB"
                     /locus_tag="OHO85_000490"
     CDS             complement(18640..19635)
                     /gene="meaB"
                     /locus_tag="OHO85_000490"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCQ2669919.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DTIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIINFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19628..21772)
                     /gene="scpA"
                     /locus_tag="OHO85_000491"
     CDS             complement(19628..21772)
                     /gene="scpA"
                     /locus_tag="OHO85_000491"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCQ2669920.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEEVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(21976..22869)
                     /gene="argP"
                     /locus_tag="OHO85_000492"
     CDS             complement(21976..22869)
                     /gene="argP"
                     /locus_tag="OHO85_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCQ2669921.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            23011..23241
                     /locus_tag="OHO85_000493"
     CDS             23011..23241
                     /locus_tag="OHO85_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2669922.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RLLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            23297..23956
                     /gene="rpiA"
                     /locus_tag="OHO85_000494"
     CDS             23297..23956
                     /gene="rpiA"
                     /locus_tag="OHO85_000494"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCQ2669923.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24212..25444
                     /gene="serA"
                     /locus_tag="OHO85_000495"
     CDS             24212..25444
                     /gene="serA"
                     /locus_tag="OHO85_000495"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCQ2669924.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTQ
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25673..25732
                     /gene="ibsC"
                     /locus_tag="OHO85_000496"
     CDS             25673..25732
                     /gene="ibsC"
                     /locus_tag="OHO85_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCQ2669925.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            26065..26124
                     /locus_tag="OHO85_000497"
     CDS             26065..26124
                     /locus_tag="OHO85_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCQ2669926.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26225..26827)
                     /locus_tag="OHO85_000498"
     CDS             complement(26225..26827)
                     /locus_tag="OHO85_000498"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCQ2669927.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26848..27031)
                     /gene="ssrS"
                     /locus_tag="OHO85_000499"
     ncRNA           complement(26848..27031)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OHO85_000499"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(27073..27402)
                     /gene="zapA"
                     /locus_tag="OHO85_000500"
     CDS             complement(27073..27402)
                     /gene="zapA"
                     /locus_tag="OHO85_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCQ2669928.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27570..28148
                     /gene="ygfB"
                     /locus_tag="OHO85_000501"
     CDS             27570..28148
                     /gene="ygfB"
                     /locus_tag="OHO85_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCQ2669929.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            28174..29499
                     /gene="pepP"
                     /locus_tag="OHO85_000502"
     CDS             28174..29499
                     /gene="pepP"
                     /locus_tag="OHO85_000502"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCQ2669930.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29496..30674
                     /gene="ubiH"
                     /locus_tag="OHO85_000503"
                     /gene_synonym="visB"
     CDS             29496..30674
                     /gene="ubiH"
                     /locus_tag="OHO85_000503"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCQ2669931.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFATLAAEDYQLAVL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30697..31899
                     /gene="ubiI"
                     /locus_tag="OHO85_000504"
                     /gene_synonym="visC"
     CDS             30697..31899
                     /gene="ubiI"
                     /locus_tag="OHO85_000504"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCQ2669932.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDTTLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELVAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAAMMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31973..32056)
                     /locus_tag="OHO85_000505"
     CDS             complement(31973..32056)
                     /locus_tag="OHO85_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2669933.1"
                     /translation="MSKNTKSKIRDIRKYNAKTEVKLVYFK"
     gene            32347..33441
                     /gene="gcvT"
                     /locus_tag="OHO85_000506"
     CDS             32347..33441
                     /gene="gcvT"
                     /locus_tag="OHO85_000506"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCQ2669934.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            33465..33854
                     /gene="gcvH"
                     /locus_tag="OHO85_000507"
     CDS             33465..33854
                     /gene="gcvH"
                     /locus_tag="OHO85_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCQ2669935.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            33972..36845
                     /gene="gcvP"
                     /locus_tag="OHO85_000508"
     CDS             33972..36845
                     /gene="gcvP"
                     /locus_tag="OHO85_000508"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCQ2669936.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFEPGHSVV
                     QIEGMLTRQGAVSAAPFGGASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFLVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            37111..37854
                     /locus_tag="OHO85_000509"
     CDS             37111..37854
                     /locus_tag="OHO85_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCQ2669937.1"
                     /translation="MAIALVTGGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(37911..39344)
                     /gene="bglA"
                     /locus_tag="OHO85_000510"
     CDS             complement(37911..39344)
                     /gene="bglA"
                     /locus_tag="OHO85_000510"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCQ2669938.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            39389..39700
                     /locus_tag="OHO85_000511"
     CDS             39389..39700
                     /locus_tag="OHO85_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCQ2669939.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            39864..40523
                     /locus_tag="OHO85_000512"
     CDS             39864..40523
                     /locus_tag="OHO85_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCQ2669940.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(40600..41580)
                     /gene="ygfZ"
                     /locus_tag="OHO85_000513"
     CDS             complement(40600..41580)
                     /gene="ygfZ"
                     /locus_tag="OHO85_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCQ2669941.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            41823..42089
                     /gene="sdhE"
                     /locus_tag="OHO85_000514"
     CDS             41823..42089
                     /gene="sdhE"
                     /locus_tag="OHO85_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCQ2669942.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            42070..42477
                     /locus_tag="OHO85_000515"
     CDS             42070..42477
                     /locus_tag="OHO85_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCQ2669943.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(42517..43038)
                     /gene="fldB"
                     /locus_tag="OHO85_000516"
     CDS             complement(42517..43038)
                     /gene="fldB"
                     /locus_tag="OHO85_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCQ2669944.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            43150..44046
                     /gene="xerD"
                     /locus_tag="OHO85_000517"
     CDS             43150..44046
                     /gene="xerD"
                     /locus_tag="OHO85_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCQ2669945.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMIEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            44071..44781
                     /gene="dsbC"
                     /locus_tag="OHO85_000518"
     CDS             44071..44781
                     /gene="dsbC"
                     /locus_tag="OHO85_000518"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCQ2669946.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            44787..46520
                     /gene="recJ"
                     /locus_tag="OHO85_000519"
     CDS             44787..46520
                     /gene="recJ"
                     /locus_tag="OHO85_000519"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCQ2669947.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            46611..47709
                     /gene="prfB"
                     /locus_tag="OHO85_000520"
     CDS             join(46611..46685,46687..47709)
                     /gene="prfB"
                     /locus_tag="OHO85_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCQ2669948.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            47719..49236
                     /gene="lysS"
                     /locus_tag="OHO85_000521"
     CDS             47719..49236
                     /gene="lysS"
                     /locus_tag="OHO85_000521"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCQ2669949.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(49279..49827)
                     /gene="idi"
                     /locus_tag="OHO85_000522"
     CDS             complement(49279..49827)
                     /gene="idi"
                     /locus_tag="OHO85_000522"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCQ2669950.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            49882..49953
                     /locus_tag="OHO85_000523"
     CDS             49882..49953
                     /locus_tag="OHO85_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2669951.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKSI"
     gene            complement(49950..50075)
                     /locus_tag="OHO85_000524"
     CDS             complement(49950..50075)
                     /locus_tag="OHO85_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2669952.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(50077..51525)
                     /gene="uacT"
                     /locus_tag="OHO85_000525"
     CDS             complement(50077..51525)
                     /gene="uacT"
                     /locus_tag="OHO85_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCQ2669953.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            51961..53880
                     /gene="ygfT"
                     /locus_tag="OHO85_000526"
     CDS             51961..53880
                     /gene="ygfT"
                     /locus_tag="OHO85_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HCQ2669954.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     VAQPSRSAALLPVNSRKGADKISASERKNHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVTIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSNL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            53880..54368
                     /locus_tag="OHO85_000527"
     CDS             53880..54368
                     /locus_tag="OHO85_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCQ2669955.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(54404..55771)
                     /gene="ghxQ"
                     /locus_tag="OHO85_000528"
                     /gene_synonym="ygfQ"
     CDS             complement(54404..55771)
                     /gene="ghxQ"
                     /locus_tag="OHO85_000528"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HCQ2669956.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(55807..57123)
                     /gene="guaD"
                     /locus_tag="OHO85_000529"
     CDS             complement(55807..57123)
                     /gene="guaD"
                     /locus_tag="OHO85_000529"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HCQ2669957.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWENGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(57141..58541)
                     /gene="xanQ"
                     /locus_tag="OHO85_000530"
     CDS             complement(57141..58541)
                     /gene="xanQ"
                     /locus_tag="OHO85_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HCQ2669958.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(58706..61576)
                     /locus_tag="OHO85_000531"
     CDS             complement(58706..61576)
                     /locus_tag="OHO85_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HCQ2669959.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKRDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(61573..62352)
                     /locus_tag="OHO85_000532"
     CDS             complement(61573..62352)
                     /locus_tag="OHO85_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HCQ2669960.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLASPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(62403..63731)
                     /gene="ssnA"
                     /locus_tag="OHO85_000533"
     CDS             complement(62403..63731)
                     /gene="ssnA"
                     /locus_tag="OHO85_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HCQ2669961.1"
                     /translation="MLILKNVTAVQLHPAKVQEAVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFAAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDTLA"
     gene            complement(63734..66832)
                     /gene="ygfK"
                     /locus_tag="OHO85_000534"
     CDS             complement(63734..66832)
                     /gene="ygfK"
                     /locus_tag="OHO85_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HCQ2669962.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVKKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(67154..67732)
                     /gene="mocA"
                     /locus_tag="OHO85_000535"
     CDS             complement(67154..67732)
                     /gene="mocA"
                     /locus_tag="OHO85_000535"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCQ2669963.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITAQKRYTKI"
     gene            67835..68605
                     /gene="yqeC"
                     /locus_tag="OHO85_000536"
     CDS             67835..68605
                     /gene="yqeC"
                     /locus_tag="OHO85_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCQ2669964.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            68653..70278
                     /locus_tag="OHO85_000537"
     CDS             68653..70278
                     /locus_tag="OHO85_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCQ2669965.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFI
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(70319..71251)
                     /gene="arcC"
                     /locus_tag="OHO85_000538"
     CDS             complement(70319..71251)
                     /gene="arcC"
                     /locus_tag="OHO85_000538"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCQ2669966.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(71299..72684)
                     /gene="hyuA"
                     /locus_tag="OHO85_000539"
     CDS             complement(71299..72684)
                     /gene="hyuA"
                     /locus_tag="OHO85_000539"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HCQ2669967.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNIREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(72737..73948)
                     /locus_tag="OHO85_000540"
     CDS             complement(72737..73948)
                     /locus_tag="OHO85_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCQ2669968.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(74006..75202)
                     /gene="dpaL"
                     /locus_tag="OHO85_000541"
     CDS             complement(74006..75202)
                     /gene="dpaL"
                     /locus_tag="OHO85_000541"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCQ2669969.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(75260..76447)
                     /gene="ygeW"
                     /locus_tag="OHO85_000542"
     CDS             complement(75260..76447)
                     /gene="ygeW"
                     /locus_tag="OHO85_000542"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCQ2669970.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            76926..78704
                     /locus_tag="OHO85_000543"
     CDS             76926..78704
                     /locus_tag="OHO85_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HCQ2669971.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVTEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(78744..79223)
                     /gene="xdhC"
                     /locus_tag="OHO85_000544"
     CDS             complement(78744..79223)
                     /gene="xdhC"
                     /locus_tag="OHO85_000544"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCQ2669972.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(79220..80098)
                     /gene="xdhB"
                     /locus_tag="OHO85_000545"
     CDS             complement(79220..80098)
                     /gene="xdhB"
                     /locus_tag="OHO85_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCQ2669973.1"
                     /translation="MFDFASYHRAATLADAINLLVDNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPVTQRHLPALCAAASSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHVGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(80109..82406)
                     /gene="xdhA"
                     /locus_tag="OHO85_000546"
     CDS             complement(80109..82406)
                     /gene="xdhA"
                     /locus_tag="OHO85_000546"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCQ2669974.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            82821..83576
                     /locus_tag="OHO85_000547"
     CDS             82821..83576
                     /locus_tag="OHO85_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCQ2669975.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSITRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            83655..83728
                     /locus_tag="OHO85_000548"
     tRNA            83655..83728
                     /locus_tag="OHO85_000548"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:83687..83689,aa:Gly,seq:ccc)
     gene            83962..85572
                     /locus_tag="OHO85_000549"
     CDS             83962..85572
                     /locus_tag="OHO85_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2669976.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHVKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPNP
                     IIPLNWSKASMTLMLKEMSSLINDEGNK"
     gene            85639..85818
                     /locus_tag="OHO85_000550"
     CDS             85639..85818
                     /locus_tag="OHO85_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HCQ2669977.1"
                     /translation="MINKLLLAYLIGLVVTSTFIFIFSEEKVTYRLFATIITGLTWPL
                     SLIPSIISLMIRKSD"
BASE COUNT        21023 a        21241 c        22683 g        21028 t
ORIGIN      
        1 atttgttgtt atatctataa ctcaatgtta tataagaaat taataattca ctgttttcaa
       61 aacatcggtt tccctgctca attgctttca ttaaaccgca cgagtatttt atttttaccg
      121 ccggacggta ccctaaaaat aaaataagga taatttatgc ggcttagtga ttattttcca
      181 gaatcatcaa tctcagttat acattcagca aaagattggc aggaagctat cgatttctcg
      241 atggtatcat tgctggataa aaactatatc agcgagaatt acattcaggc aattaaagat
      301 tccaccatta acaatggccc ttattatatt ctcgcaccag gcgtggcaat gcctcatgcg
      361 cgaccggaat gtggggcgct taaaaccggg atgtctttga cattacttga acaaggtgtt
      421 tattttccgg ggaatgacga accaattaaa ttactcatcg gactctctgc tgccgatgcc
      481 gattcgcaca ttggcgctat tcaggcgtta agtgaattac tgtgcgaaga agaaatactc
      541 gaacaactct taacagcatc atcagaaaaa caattagcgg acattatcag ccgcggataa
      601 ttactctctc ctctacagga taatattatg gaaaacaagt ctgctcgtgc aaaggtccag
      661 gcttttgggg gctttttgac tgcaatggtc atccccaata ttggtgcttt tattgcctgg
      721 ggcttcataa ctgcgttatt tattcccacc ggttggctgc cgaatgaaca tttcgccaaa
      781 attgtcggcc cgatgattac ctatttattg cccgtgatga ttggttctac tggtggtcat
      841 ctggtcggcg gtaaacgcgg ggcggtcatg ggcggaatag gtactattgg tgtgatcgtt
      901 ggcgcagaga tcccgatgtt ccttggctca atgatcatgg ggccgctcgg tgggttggtc
      961 ataaaatatg tcgataaggc actggaaaaa cgcatacctg ccggttttga gatggttatc
     1021 aataacttct cattaggtat cgcggggatg ctcctttgtc tgctgggttt tgaagtgatc
     1081 ggcccggcgg tgttaattgc caatactttc gtcaaagagt gtatcgaggc gctggtacat
     1141 gcgggttatc tgcctctgct gtcagtcatc aatgaaccgg cgaaagtgct tttccttaat
     1201 aatgcgatcg atcagggcgt ctattatccg ctgggaatgc aacaggcttc ggttaacggt
     1261 aaatccatct tctttatggt ggcctctaac ccaggtccgg gcctggggct gctgctggcg
     1321 tttaccttgt ttggtaaagg gatgagtaaa cgttctgcgc ccggggcgat gattattcac
     1381 ttcctcggtg ggatccacga actgtatttc ccatatgtgc tgatgaagcc gctgactatt
     1441 attgccatga ttgcgggcgg tatgtctggt acctggatgt ttaacttact ggacggtggt
     1501 ctggtggctg gcccaagtcc ggggtctatc tttgcttacc tggcactgac gccgaaaggc
     1561 tcgttcctgg cgacaattgc cggtgttacg gtaggtaccc tggtgtcctt tgctattact
     1621 tcgctgatac tgaagatgga aaaaacggtg gaaacggaga gcgaagatga gtttgctcag
     1681 tcagccaatg cggttaaggc gatgaaacaa gagggtgcat tctcgttaag cagggttaag
     1741 cgtatcgcct ttgtttgcga tgcggggatg ggctccagtg cgatgggcgc gaccaccttc
     1801 cgtaaacgcc tggaaaaagc ggggctggca atagaagtaa aacattacgc gatagaaaac
     1861 gtgcctgcgg atgccgatat cgtcgttact catgccagtc tggaagggcg cgtgaaacgt
     1921 gttacggata aaccgctgat attgattaat aactatattg gcgatccaaa actcgacact
     1981 ttatttaatc aattaaccgc cgaacataaa cactgattac agaggtaaaa atgaaaacca
     2041 aagttgctgc tatttatggc aagcgggatg tccgtctgcg cgaatttgaa ctgccagaaa
     2101 ttaccgataa tgaactgtta gtgagtgtaa tttctgacag cgtctgttta tcgacctgga
     2161 aagcggcgtt actcggtagt gaacataaac gcgtacccga cgatttagaa aatcatccgg
     2221 tcattaccgg gcatgaatgt gccggggtta ttgtcgaagt gggtaaaaat ctcactggca
     2281 aatataaaaa aggccagcgt tttgtattgc aaccggcgat ggggttacca agcggatatt
     2341 cagcaggcta cagctacgaa tattttggcg gtaacgccac ctatatgatt attcccgaaa
     2401 tcgccattaa tttgggctgc gtattaccgt atcacggctc ttattttgct gcggcgtcgc
     2461 tggcagagcc tatgtgctgc attattggtg cttatcatgc caattatcac accacgcaat
     2521 atgtttatga gcatcgcatg ggcgtcaaac ctggcggcaa tattgcactg ctggcgtgtg
     2581 cgggtccgat gggcattggc gctatcgatt acgccattaa cggtggcata caaccgtcac
     2641 gggtggtggt ggtcgatatc gacgacaaac gcctggcgca ggtgcagaag ctgctgccgg
     2701 tggatctggc ggccagtaaa ggcattgagc tggtgtatgt gaataccaaa gggatgagcg
     2761 atccggtcca gacgctgcgg gcgctgacag gagatgtcgg gttcgatgac atttttgttt
     2821 atgcggcggt gcctgctgtc gttgagatgg ctgacgaatt actggcggaa gatggctgtc
     2881 tgaacttctt tgccgggccg acggataaaa acttcaaagt gccgtttaat ttctacaacg
     2941 tccattacaa cagcacgcac gtagtcggta catccggcgg ttcaacggac gacatgaaag
     3001 aggcgattgc gttaagcgcc acggggcagt tgcagccgtc ctttatggtc acgcatatcg
     3061 gtgggctgga tgcggtgcca gataccgtgc tcaatctgcc ggatatccct ggcggtaaaa
     3121 aactcattta taacggcgtg accatgccgc tcactgccat tgccgatttt gccgaaaaag
     3181 gcaaaaccga tccgctgttt aaagagttgg cgcggctggt tgaggaaacg cacggcatct
     3241 ggaatgaaca ggccgagaaa tatctgctgg cacaatttgg cgttgatatc ggggaggccg
     3301 cgcaatgatg tccctggcgt ggccattatt tcgcgttacg gaacaggcag cgttggctgc
     3361 ctggccgcaa accggatgtg gcgacaaaaa taaaattgat ggcctggcgg tcaccgcgat
     3421 gcgccaggca ttaaacgacg tcgcttttcg tggacgagtg gttatcggcg aaggagaaat
     3481 tgaccatgcg ccgatgctgt ggattggcga agaggtgggc aaaggcgatg ggccagaagt
     3541 cgatatcgcg gttgacccga ttgaaggcac gcggatggta gcgatggggc agagcaatgc
     3601 gctggcggtc atggctttcg cccctcgaga tagcctgtta catgcgcccg atatgtatat
     3661 gaaaaagctg gttgttaatc gactggctgc cggggcgatt gatctgtctt tgcctctggc
     3721 ggacaacttg cgtaacgtgg cgcgggcgtt aggtaaaccg ctggacaagc tgcgcatggt
     3781 tacgctggat aaaccgcgcc tgagtgccgc gattgaagaa gcaacacagc tgggcgtgaa
     3841 ggttttcgct ctgccggatg gcgatgtcgc cgccagcgtg ctgacctgct ggcaggataa
     3901 tccttacgac gtgatgtaca ccatcggcgg tgcgccagaa ggcgtgattt ctgcctgtgc
     3961 cgttaaagca ttaggtgggg atatgcaggc cgagctgatt gacttttgtc aggctaaagg
     4021 ggattacacg gaaaatcggc agattgcaga gcaggagcgc aagcgttgta aggcaatggg
     4081 cgttgacgtt aaccgtgtgt actcgctcga tgaactggtg agggggaacg atatcctctt
     4141 tagcgctacg ggcgtgacgg gcggcgagtt ggtgaacggt atccaacaga cggcgaatgg
     4201 ggtgcggacg cagacattac tgatcggcgg cgcggaccaa acgtgtaata taatagactc
     4261 cctgcattga tggtgatcta tcggaagacc aatggcgacg ctgacagaag atgatgtgct
     4321 tgagcaactg gatgcacagg acaatttatt ttcatttatg aaaactgcgc attctatttt
     4381 gctcctgggg atacgccagt ttctgccgtc gctgtttgtc gataacgatg aagagatcgt
     4441 cgaatatgca gtgaagccgt tactcgccca aagcggcccg cttgacgata ttgatgttgc
     4501 gctgcgtttg atttatgcgc tggggaaaat ggataaatgg ctgtatgccg atatcacgca
     4561 cttttcccag tactggcatt acctgaacga acaggatgaa acgcccggat ttgccgatga
     4621 catgacctgg gattttatca gcaacgtcaa tagcataacc tgcaatgcga cgctctatga
     4681 cgcgttaaaa gcgatgaagt tcgccgattt cgccgtctgg tcagaggcgc gttttagcgg
     4741 aatggtcaaa acggcgctga cgctggcagt aacgaccacc ttaaaggaat taacgccgtg
     4801 aaaattgaat taacggtgaa tgggctgaac gtgcaagcgc agtaccatga tgaagagata
     4861 gaacgcgtgc ataaaccgtt gctgcgtatg ctggcggcct tacagacggt aagtccgcag
     4921 cgacgtacgg tggtttttct ttgcgctccg ccggggacgg gtaaatctac cttaactacc
     4981 ttctgggaat atctcgcgca acaagaccct gaattacccg ctatccagac gctcccgatg
     5041 gatggttttc accattacaa tagctggctg gatgcgcatc aattgcgccc cttcaaaggc
     5101 gcaccagaga catttgacgt tgcgaaactg gcggaaaatc tgtgccaggt agtggaaggg
     5161 gattgtacgt ggccgcagta tgatcgacaa aagcatgatc ctgttgaaga tgcgttgcac
     5221 gttaccgcac cactcgtcat cgtcgaagga aactggttgt tgctggacga cgagaagtgg
     5281 tgccagcttg cgcaattttg tgatttctcg atctttatca acgcgccagc tactgcgttg
     5341 cgggaacgac tggtagggcg caagctggcg ggaggggtat cgctggcaga cgcagaagct
     5401 ttttatgacc gtaccgacgg gccgaatgtt cgccgggtgc tggaagagag tctgcctgca
     5461 aatttgacct taatgatgac cgctacgggt gaatatcgtc tggtggatta acgttcctga
     5521 ttagaccatc ttccagtcgc accacacgag agaaatgacg ccgggtaaat gcggcgtcat
     5581 gactgattgc tacgacgctc gtgcctcgct ggccgcactt ctccagccag ctttcaaaca
     5641 cgctcaacca attttcgtca aaatcccgac tcggctcgtc cagcagcaat agtggcggag
     5701 agagtgcttc caggcaggcg acggcgacca tccgtcgctg cgcactatgc agatccaacg
     5761 ggtgcgagtt ggcagtgtct gctaactgac aacattgcag ggcagcatgg gtacgttgag
     5821 tgatttcatc cgccgggcat ttttgcagtt tcaaaccaaa tgcgatctcg tcagcgacgg
     5881 tgctgtgaaa aagttggttt tccgcttcct gaaacagtac gccaaccttt gccgcgcgct
     5941 ggcggttttt caggtttttc atcgcctgtt gttgcagcat aaccgtaccg gctgtgggcg
     6001 taagaagacc tgccattact cgtaacagcg tcgattttcc cgcgccattg tcgccggtca
     6061 gcgccaacca ttcaccttgt ttgagttgca gagaaatgtc gcaaagacaa tccgttgccg
     6121 cgccaggcca gcgataagat atcttattta acgttaacat agtggctgga gcgctccgtt
     6181 ttgcaattgc cacgcttgct ggcagtagtt caggaaaggt gtgcgatgac gttcaaaaag
     6241 aataatcagc gaaccacgtt caaacgccca gtgttgtaag cgctgcaaca gcatttcgct
     6301 ggcctgcgga gtcaggcggc taaaggcttc atcaagaatt aagagctttg gctgcatcgc
     6361 gatggcgcag gcgatgacca cgcgctgtgt ctctccaccg gagagtgttg ccgggtgacg
     6421 atggcgcaac ggctggcact cggtcagagt cagggcggca tcaatccgcg ccataatctc
     6481 tgcttcagca agacacagat tttctggccc aaaggcaact tcttcttcca cgctaaaggt
     6541 acagccggag agctgcaaat agggcgattg ctggacgagc tggatggtgg cggattgttc
     6601 attaagcggg agatgcccaa tgggcgtacc gaggagcgtc cccgtgcctg ttatctctcc
     6661 tggcagaaag tccggatacc agcccgccat cagctgtgcc agcgtacttt tgccactgcc
     6721 gttgtcacca aagatggcga ccatccccgg tttgacatag tgaaagtcat aacagaacgg
     6781 aggatgcgtt gagtgtgtcg ggcaataacg gaactgttct aacgtaacca tatccacgct
     6841 ccgaattctg tcagcatcag caagatcatg gtgtaacgcg ctacgcgttg tagggtgcta
     6901 tcggctggtg cccacaacgt tgttcgtcgg ttgtggatcc gaaatgcccg catatccagc
     6961 gccgcaccac ggacagccag atcgttaagc gcattgtgtg ttagcggaat aattaacgcg
     7021 ggcatcgcac gtaaacgctg ataccagccc tcatccagcg gcacgccgcg ggcacgctgc
     7081 gcttcatgaa taatcgctaa ttgccgtttt aactgctcaa cgaccaataa cggcccggca
     7141 aacagataag cgacgcctgg cggcaggcga gaagcaaaca gagcgcgaat aaaacattgc
     7201 acggggacgt actgcatcca caactgtgag gttgaaacaa tcgccagaat ccgcaaccag
     7261 agcgtaatag catgcgacca ccgttctggc gaacgcggcg tgccgcttag ccattctgtt
     7321 agccagccac cgtgtaccag ccacagcccg gctcccagcg agaacatcag ccagacgaca
     7381 tattttgccc gccgccgcgt ggcctttagc gcaataaggc aaaaaaaggt cgccgcgcta
     7441 tagatcggca aaatggtctg cgcgggcagg ataagcgtgg tgcaggccgc caatgcccat
     7501 agtgttaatg aggtaaatgg atgcatttag cgcacggcag ccattgccgg aaaatggcgc
     7561 gtggtgcgaa tcggcagctg gcgcagtaaa agccagacaa tcaccgctgt aagaatttta
     7621 tcgaccaggt tcgcgcctat caccgtaatc gccaccgact ccaccaggtt ttgccccata
     7681 gagtgcatcc aggcaacgaa caaatcggct ccgctaccgg tgacaccgcc gaacagcgca
     7741 gtacgcagcg gtacagcgac gaccgttacc gcaagcgtaa taatcacgcc gctgacaacg
     7801 acttttggca gggtgcgaaa ccaacccgct cgcgccagcc aaccggcgac caggccgatg
     7861 accatcgcga cgggggcaaa tgcggcggcg atcgggtcgg tcagcaatcc ccagagtaaa
     7921 ttggtcagta agccggttag catcccgatc accggaccaa gcagtaccgc gctaataagg
     7981 gtgccgatgg agtcgagaaa tataggtaat ttgaccatgc tggccagttg cccgccgatc
     8041 atattgatgg cgatagatat aacgatcaac accagtgcct gactggaaaa gtggctacgc
     8101 gccataagaa aacctctctg aaagtagtta tttaacgggg gaaatatgca actcgtcgta
     8161 accagaaagc gcgcaaggtt ggcggctaaa cgccagttcc tcaaggctac cgatcactag
     8221 ttcaagcgtg gtccgcacgg tacagccggg catcatatga ccgccaagca tcgtgccgtg
     8281 tggatcggaa acgcagagat gaagatgctc accactctgt tccagcgtgc cgtttagtga
     8341 gatcacttcg aatgtaccgt tcaggagcgt ggtgccttct tgcccggcat agcgcaaggc
     8401 gacgtcagtc aggctaccgg tacagcctgc aatccaggcg gcatgaagtt gctgctgctg
     8461 tgcaaaagcg tgtagctggg aaaaaacttc ctgcccgggg agcaggcgca gtgcgtaaaa
     8521 gcgtgcggag gaagaatatg ggtagggcaa tgtcatcatt ggagtactcc gaaactcgga
     8581 cgtattgcgg ataatatcga cgctgcatca gtcatattct gatataagtc agggtttgtg
     8641 acggcacaac gaaaagattt tgctacgctt attcgtgatt ctgcaaagtg gaataaattc
     8701 cacaggggcg gggctgaaaa cgtgacgtaa gtcagatatt tgttttcttt gactggacaa
     8761 tcattccttt tattccacgt ttcgcttatc ctagctgaag cgtttcagtc gattaaatgt
     8821 tcgacaatta accaatcagt cgcagtttgc gacaggtaag gtttccccgg acgatttgct
     8881 ggattactct gtctggcaaa tttgctcagg gaaaaccttg caggagatct atgaccgtac
     8941 gcgtagcgat aaatggcttc ggtcgcatcg ggcgtaatgt ggttcgtgct ttgtatgaat
     9001 caggacgccg tgcggaaatt accgtggtgg caatcaacga actggcggat gctgcgggca
     9061 tggcgcattt gttgaaatat gacaccagtc atggccgttt tgcatgggaa gtacgacagg
     9121 aacgcgatca actttttgtt ggtgatgacg ccatccgcgt attgcatgaa cgttcactgc
     9181 aatcgctccc ctggcgtgaa cttggcgttg atgtagtcct cgactgcacc ggcgtatatg
     9241 gctcccgcga gcatggcgaa gcgcatattg ccgccggggc caaaaaagtg ctcttttcac
     9301 atcctggcag taacgatctc gacgcgaccg ttgtttacgg cgtcaatcag gatcaacttc
     9361 gtgcggaaca ccgcatcgtt tctaacgctt cctgtaccac gaattgcata attcccgtca
     9421 tcaaattgtt agatgatgcg tacggtattg agtccggcac tgtgaccaca attcactccg
     9481 ccatgcacga tcaacaggtt attgatgcat accatcctga cctgcgtcgc acccgggcag
     9541 ccagccagtc gatcattccg gtcgatacta aactggccgc cggtatcaca cgattttttc
     9601 cgcaatttaa cgatcgcttt gaagcgattg cggtacgtgt gccaaccata aatgtgacgg
     9661 caatcgattt aagcgtgacg gtgaagaaac ctgtaaaagc caatgaagtc aacctgttgc
     9721 tgcaaaaagc agcacaaggt gcatttcatg gtatagttga ctatacggaa ttgccgttgg
     9781 tctctgtaga ttttaaccac gatccgcaca gtgccattgt cgatggcacc caaacccggg
     9841 tcagtggcgc acacctgatc aaaacgttgg tctggtgcga taacgaatgg ggctttgcta
     9901 accgaatgct cgacacgacg ttagctatgg ctactgttgc tttcaggtaa gacgcaagca
     9961 gcgtctgcaa aacttttaga atcaacgaga ggattcacca tgtctgtaat taagatgacc
    10021 gatctggatc ttgctgggaa acgtgtattt atccgtgcgg atctgaacgt accagtaaaa
    10081 gacgggaaag taaccagcga cgcgcgtatc cgtgcttctc tgccgaccat cgaactggcc
    10141 ctgaaacaag gcgcaaaagt gatggtaact tcccacctgg gtcgtcctac cgaaggcgag
    10201 tacaacgaag aattctctct gctgccggtt gttaactacc tgaaagacaa actgtctaac
    10261 ccggttcgcc tggttaaaga ttacctcgac ggcgttgacg ttgctgaagg tgaactggtt
    10321 gttctggaaa acgttcgctt caacaaaggc gagaagaaag acgacgaaac cctgtccaaa
    10381 aaatacgctg cactgtgtga cgtgttcgta atggacgcat tcggtactgc tcaccgcgcg
    10441 caggcttcta ctcacggtat cggtaaattc gctgacgttg cgtgcgcagg cccgctgctg
    10501 gcagctgaac tggacgcgct gggtaaagca ctgaaagaac ctgctcgccc gatggtggct
    10561 atcgttggtg gttctaaagt atctaccaaa ctgaccgttc tggactcctt gtctaaaatc
    10621 gctgaccagc tgattgttgg tggtggtatc gctaacacct ttatcgcggc acaaggccac
    10681 gatgtgggta aatccctgta cgaagctgac ctggttgacg aagctaaacg tctgctgacc
    10741 acctgcaaca tcccggttcc gtctgatgtt cgcgtagcaa ccgagttctc tgaaactgca
    10801 ccggctaccc tgaaatctgt taacgatgtg aaagctgacg agcagatcct ggatatcggt
    10861 gatgcttccg ctcaggaact ggctgaaatc ctgaagaatg cgaaaaccat tctgtggaac
    10921 ggtccggttg gcgtgttcga attcccgaac ttccgcaaag gtactgaaat cgtggctaac
    10981 gctatcgcag acagcgaagc gttctccatc gctggcggcg gcgacactct ggcagcaatt
    11041 gacctgttcg gcattgctga caaaatctcc tacatctcca ctggcggcgg cgcattcctc
    11101 gaattcgtgg aaggtaaagt actgcctgca gtagcgatgc tcgaagagcg cgctaagaag
    11161 taaaaaatca cagggcaggg aaacctgccc ttgtttcagc gcgcttttag agcacgcatt
    11221 ttttcatact ctaaataatt cgagttgcag gaaggcgaca agagagtgaa tccccaggag
    11281 cttacataag taagtgactg gggtgagcga acgcagacgc agcacatgca acttgaagta
    11341 tgacgagtat aaggcccgac gatacaggac aagagacatg tctaagattt ttgatttcgt
    11401 aaaacctggc gtaatcactg gtgatgacgt acagaaagtt ttccaggtag caaaagaaaa
    11461 caacttcgca ctgccagcag taaactgcgt cggtactgac tccatcaacg ccgtactgga
    11521 aaccgctgct aaagttaaag cgccggttat cgttcagttc tccaacggtg gtgcttcttt
    11581 tatcgctggt aaaggcgtga aatctgacgt tccgcagggc gctgctatcc tgggcgcgat
    11641 ctctggtgcg catcacgttc accagatggc tgaacattat ggtgttccgg ttatcctgca
    11701 cactgaccac tgcgcgaaga aactgctgcc gtggatcgac ggtctgctgg acgcgggtga
    11761 aaaacacttc gctgctaccg gtaagccgct gttctcttct cacatgatcg acctgtctga
    11821 agaatctctg caagagaaca tcgaaatctg ctctaaatac ctggagcgta tgtccaaaat
    11881 cggcatgact ctggaaatcg aactgggttg caccggtggt gaagaagacg gcgtggacaa
    11941 cagccacatg gacgcttctg cactgtacac ccagccggaa gacgttgatt acgcatacac
    12001 cgagctgagc aaaatcagcc cgcgcttcac catcgcagcg tccttcggta acgtacacgg
    12061 tgtttacaag ccgggtaacg tggttctgac tccgaccatc ctgcgtgatt ctcaggaata
    12121 tgtttctaag aaacacaacc tgccgcacaa cagcctgaac ttcgtattcc acggtggctc
    12181 cggttctact gctcaggaaa tcaaagattc cgtaagctac ggcgtagtga aaatgaacat
    12241 cgataccgat acccaatggg caacctggga aggcgttctg aactactaca aagcgaacga
    12301 agcttatctg caggggcagc tgggtaaccc gaaaggcgaa gatcagccga acaagaaata
    12361 ctacgatccg cgcgtatggc tgcgtgccgg tcagacttcg atgattgctc gtctggaaaa
    12421 agcattccag gaactgaacg cgatcgacgt tctgtaagat attcctttct gcttatctca
    12481 aggcccgctc tgcgggtctt tttttcgcca aaagcaaaat tgcctgatgc gctacgctta
    12541 tcaggcctac acgtatcctg caatatattg aatttgcagg tttttgtagg ccggataagg
    12601 cgttcacgcc gcatccggca tgacaaagcg cacattgata tctgtctgag ccccgctctg
    12661 cggggatttt ttcgccaaaa gcaaggtgat tcagatgaga aatctgtgat ctatttggca
    12721 aaattatgct ttattgttta cccttgtcag actgcccgtc ataaggcggc ggagtgtatt
    12781 tctccatttt gagtcagttg aaaaggaata ttgaatggaa gatttgaatg ttgtcgatag
    12841 cataaacggc gcgggaagct ggctggtagc taaccaggcg ctgctgctaa gttatgcagt
    12901 aaacatcgtg gcggcactcg cgatcatcat cgttggtttg attatcgcgc ggatgatttc
    12961 caacgcggtg aatcgcctga tgatctcccg taaaatcgat gccactgttg ctgattttct
    13021 ttctgcatta gtccgttacg gtattatcgc ctttacgcta atcgctgccc tgggacgcgt
    13081 gggtgtacaa acagcgtcag tcattgctgt actcggtgcc gcaggcttag ctgttggtct
    13141 ggctttgcag gggtcacttt ctaacctggc cgctggcgtg ttacttgtca tgttccgccc
    13201 gttccgtgcc ggagaatatg ttgacctcgg cggcgtagcc ggtactgtgc tgagtgtgca
    13261 gattttctcc accaccatgc gtactgcaga cggtaaaatt atcgttattc cgaacggtaa
    13321 aattattgcc ggaaatatta ttaacttctc ccgcgagcca gttcgccgta acgaatttat
    13381 tattggcgtg gcgtatgatt ccgatatcga tcaggttaag cagatcctga ccaatattat
    13441 ccagtctgaa gatcgcattt tgaaagatcg cgaaatgact gtgcgcctga acgaacttgg
    13501 tgcatcgtcg attaatttcg tggtccgcgt ctggagcaac agcggcgatc tgcaaaacgt
    13561 gtactgggat gtgctggagc gtattaaacg tgaatttgat gccgccggta tcagcttccc
    13621 gtacccgcaa atggatgtga actttaagcg ggtgaaagaa gacaaagctg cgtaatcaac
    13681 gctgaggcca gataatactc tctggcctct ctcttattag ttattctgat tgccaattaa
    13741 tattatcaat ttccgctaat aacaatcccg cgatatagtc tctgcatcag atacttaatt
    13801 cggaatatcc aacgtgtttt cttattactt tcaaggtctt gctcttgggg cagctatgat
    13861 tctgccgctc ggcccacaaa atgcttttgt gatgaatcag ggcattcgtc gtcagtacca
    13921 cattatgatt gccttacttt gcgctatcag cgatttggtc ctgatttgcg cggggatttt
    13981 tggtggtagc gcgttattga tgcagtcgcc gtggttgctg gcgctggtca cctggggcgg
    14041 cgtagtcttc ttgctgtggt atggttttgg cgcttttaaa acagcgatga gtagtaatat
    14101 tgagttagct agtgccgaag tcctgaagca aggcagatgg aaaattatcg ccaccatgtt
    14161 ggcagtgacc tggctgaatc cgcatgttta cctagatact tttgttgtac tgggcagcct
    14221 tggcgggcag cttgacgtcg aaccaaaacg ttggtttgcg ctcgggacaa ttagcgcttc
    14281 tttcctgtgg ttctttggtc tggctattct cgcagcctgg ctggcaccac gcctgcgcac
    14341 ggcaaaatca cagcgcatta tcaatctggt agtgggatgt gtcatgtggt ttatagcctt
    14401 gcagctggcg agagacggta ttgctcatgc acaagccttg ttcagttagg cgctatctga
    14461 tggaaaaata aaacagaggc gctaagcttg cctccagagg tcctgaattt tgggcaagtc
    14521 aattaacacg gagagactaa cgtgaagttc aaagttatcg ccctggcggc attaatgggt
    14581 attagcggga tggcagcgca ggctaacgaa ttgccggatg gaccgcatat tgtcacctcc
    14641 ggtacggcaa gtgtggatgc ggtgccagac attgccactc ttgcgattga agttaacgtg
    14701 gccgcgaagg atgccgctac agccaagaaa caggcagatg agcgcgtcgc acaatacatt
    14761 tccttccttg aactcaatca gatcgcgaaa aaagatatca gctcagcgaa tttacgcacc
    14821 cagccagatt atgattatca ggatggtaaa agtatcctga aaggctaccg cgctgtgaga
    14881 acggtggaag tcacgctccg tcagttagac aaactgaatt ccttgctgga tggcgcgctg
    14941 aaggcgggtc ttaacgaaat tcgttcagtg tcgctgggcg tggcgcagcc ggatgcctat
    15001 aaagacaaag cgcgtaaggc agcgattgat aacgcgattc atcaggcgca ggaactggcg
    15061 aacggctttc atcgtaaact ggggccggta tatagcgtgc gctaccatgt ttccaactat
    15121 cagcccagcc caatggtgcg gatgatgaaa gccgatgccg cgccggtgtc tgcccaggaa
    15181 acttacgagc aggccgctat tcagtttgat gatcaggtcg atgtggtctt ccagttagaa
    15241 cctgtggatc aacaacccgc taaaacacct gcagcacaat aatacttgta gcccgacgta
    15301 ttcgtcgggc taatgccttt acccgatatt gcctcctttt tctgtaattt tctgtaaaac
    15361 catgacgtaa tcacctatct ttgctcgcgg tttcatcaag gataggctga ctgaaacggt
    15421 taagcggaca aggatgcggg gaggggggat ggacattttt atttcgaaaa agatgcgcaa
    15481 tttcatttta ttagcgcaaa ccaataatat tgcccgggcg gcagaaaaaa tccatatgac
    15541 ggcttcgcca tttggtaaaa gcattgccgc gctggaagaa caaattggct atacgctatt
    15601 tacccgcaaa gataataata tcagcctcaa taaagccggt caggaactgt atcaaaaact
    15661 gttcccggtt tatcaacgac tttctgcgat tgataatgaa atccataact cagggcgtcg
    15721 ttcacgagaa attgtgattg gcatagataa tacttatccg acaattattt tcgatcagtt
    15781 gattagtctc ggagataagt atgaaggcgt gaccgcccag ccagttgagt tcagcgaaaa
    15841 cggcgttatc gataatcttt ttgaccgtca gttagatttt attatctccc cacagcatgt
    15901 atctgcacga gttcaggaac tggaaaacct gactatcagt gaattaccac cgctgcgatt
    15961 gggctttctc gtttctcgcc gttatgaagg gcgtcaagag caagagctgc ttcaggagct
    16021 gccctggcta caaatgcgct ttcaaaaccg ggctaacttc gaagcaatga ttgatgcgaa
    16081 tatgcgacct tgtgggatca atcccaccat tatttatcgt ccctacagtt ttatggccaa
    16141 aatcagcgcc gtggagcgcg gacattttct gacggtgatc ccgcattttg cctggcgact
    16201 ggtgaatccg gcaacgctga aatattttga tgctccgcac aagccgatgt atatgcagga
    16261 atatctttat tcaatcagaa atcatcgtta taccgccacg atgcttcagc atattgctga
    16321 agatcgtgac gggacgagtc attaacccag catcgagccg gttgcaatta aattacggtg
    16381 taagtcgaag acgtggctaa gatcgtggtg aatatgtccg ccaggcgcat tttccagata
    16441 gcgatgcaga taatcctgat acataggatg tgcacaatta tcaatgatag tgcgggcgcg
    16501 ttgaagcggg gaaagaccgc gcagatcggc gatcccttgt tcggtgatga tcactttgac
    16561 gctgtgttcg ctgtgatcaa catggctgca cattggcacg acggttgaga tcttgccttc
    16621 tttagcaatc gacggggcca taaagatcga cagatacgcg ttgcgttcaa aatcaccgct
    16681 gccaccgatg ccgttcatca gatcgacccc ggctacgtgc gttgagttgg catgcccgta
    16741 aatatcaaac tccaggccga cgttcagagc gatgacgccc agacgacgga tgatttccgg
    16801 gttattggaa atctcctgcg gacgcaacac aatgcggctg gcaaagtaat ccatattgtc
    16861 gtaaatcttg cgcagggaat cggccgagat tgtcaggctg gaggcgctgg ccccgctgat
    16921 tttgccggtt tccagtaaat gcaccaccga ttcctgtagc acttccgaat acatcataaa
    16981 cggaggaatt tccgggtttt cccccagacg cgccattacc gcattattga tattgcccac
    17041 gccactttgc agcggcagaa attccggcgg aatacgccca tgcgccattt cctgcaacaa
    17101 gaacgtgacc acgttatcgg caatctgctg gcacatggga ttttgcttat ccagcatatt
    17161 accggcgtcg ggcaagttgg tttccacgac ggcgacaatc tttttcggat cgatttgcac
    17221 atagcgggta ccgacgcgat ccattgcatg gaagatcgac acgctattgc gccgcggtgg
    17281 cgcgccagga atcacaatat ccgccagttc tgcaacgcgc ggatcgtgat agtgattgag
    17341 ttcaatgatc actttcttcg cccgcagcag ccaggtcggc gcattaccga tcccgctggt
    17401 taaccagact cgaccatccg gtgccagtgc cgatgcttca atgacggcaa catcaatgtc
    17461 gccgaagaaa ccgtaattga ccatttgcgc cacttcgctc aaatgcaggt caacgaaact
    17521 caccgcgccc tgattgatct ttttacgtaa accggacgat gtttgatatg gcgcacgcca
    17581 ggaaacagca tcggcgtcag aaagtacatc gtcagcggcg gcgctgattg acgcacccgt
    17641 cagaaggcga atttgatacg gctttttggc ctcatgctgt tcgttagctc tgcgggcaat
    17701 cgcggtgggt agggctttcg gcgaacccgc cggggtaaag ccgctaaatg ccaccatgtc
    17761 gttatgctgg ataatttctg ccgcttcatt ggcggtcatc cttgtccact gagtttccat
    17821 ttttactcct tcgttcgcag ggattaatga ccaacgaaat taggtttacg tttttcgagg
    17881 aaagcgttca tcccttcctg gtaatcttcg ctgtcataca ccgcgcggcg catcccctga
    17941 atacgttcaa attcatcgga gttcatggtg tgtgcttcgc ccagtacacg cagctcttct
    18001 ttgataacgg caatggctaa cggcgctttc tcagagatgt ggtgcgccat ttgtaaggtg
    18061 aaatcttcca gttcttccac ttccacaaca tggttgagga tgccgacagc cagcgcgcgc
    18121 tgggcggtga ttggcgaagc ggtaaaaatc agctctttga caatgtggaa gcccgcgtcg
    18181 cgggtcaggt tgtgaatgcc gaccaggtta tacgggacgc cgaggtttac aggcgtcatt
    18241 gagaaggttg aggtactggc ggcgatgatc agatcggaac tcatgatcat ttcaaatgcg
    18301 ccaccccaaa cactaccttc caccatcgaa atgatcggtt tcgggaattt ttggatcatg
    18361 cgggtgattt gacgcaatgg atcatcatag gagagcggat cgcgaccgcc agacggcagt
    18421 tcgtgaatat cgtggcctgc ggagaagact ttggatccac tcggcgcgcg caaaatgata
    18481 cagcgaattt ccggccggtt gagatcgctt aatgcctgca taagatcatc aataaagact
    18541 ttacttaagg cattaagttt gcggccatag ttaaactcaa tgaccgccac tttgttgata
    18601 gtgacaacgt taacatactg ataagacata aaaattcctt taatcaaaat attgcgtctg
    18661 gataaattca ctgagctgcc gcaggccggt gcgcggtgag agcgtattgt ttttgaccgc
    18721 taaaagcgtc tggcggtaat agcgatcgaa atcttcattc gcgaacaggt gattcagtac
    18781 ttcttcttcg gtctgcttac gcagccattc caccgattgt tgttgccgca cttgttgtaa
    18841 acgaccactg gcagttagcg cggttttgaa gttgatgatg gcgtgccaga tctcatcgat
    18901 tccacgtttt tccagtgcgc tacaagtcag aacccgtggc tgccattcgt cgtatttacg
    18961 tcgcagaata tgcagggcac tctcgtacat atgccgggca atggcgacat tggtatggtt
    19021 atcgccatcg tctttgttga taacgagcag atcagccact tccatcagcc cttttttaat
    19081 gccctgcaga tcatcgccgc caccggcaat ttgcaacgag ataaaacagt ccaccatgcg
    19141 ggcgacttcc gtttccgact gcccgacgcc aaccgtttcg acaatcacta cgtcataacc
    19201 cgccgcttcg cataacagca ttaattcccg cgctcgctga ctggcaccgc ccagatgacc
    19261 ggaggatggt accgggcgaa taaacgccgc ttcggcacgc gccaggtcat tcatgcgggt
    19321 tttatccccg agaatgctac cgccagtgac cgggctgctg ggatcgaccg caataaccgc
    19381 gacctttaat ccctctcgaa tcaacaacat gccaaaggcc tcaagaaagg tacttttccc
    19441 cgcgccgggg gtgccggtaa cgcccagtcg cagggtgtta ccgcagtacg gcataatggt
    19501 atcaagcagc tgcgtactta gtgcctgatg acgcgggtga cggctttcca ccagcgtcat
    19561 ggcctgggcg agtgtggcac gctcaccctg acgtaagcgg cgaatacttt ctgccagcgt
    19621 ggcttcatta atcatgatgc tggcttatca gattcagtac gtcgcgcaca ctgtcgagca
    19681 taggtgtacc tggaccataa atcgccgcca cgccgcgctc ttgcaggaag gcgtaatcct
    19741 gcggcggaat gacgccaccc gcgaccacgc agatatcttc gcgtccccat tttttcagcg
    19801 cttcgaccag ttccgggatc agcgttttat gaccggcagc cagtgaggat gcgcccacta
    19861 cgtgaacgtc gttttctacg gccaggcggg cgatctcttc aggtgtagag aacatcgggc
    19921 ttaaatctac gtcgaaaccg agatcggaat aggcgctggc gatcactttc gcgccgcgat
    19981 cgtgtccatc ctggcccatc ttagcgatca gaatgcgcgg gcgacgacca ttgtcggcaa
    20041 ggaactgctc cgtttgcgca acaatggcat cgaactcgga ggccgatttc tccgactgat
    20101 gatagctttg cgcaattacg ccggtaacac actggcttgg caccagataa cggtcgaaag
    20161 cggcttccag cgcatcggaa atttcaccca gggtggcgcg aacgcgagcg gcattaacag
    20221 cggcagccag caggttttcg ttatgctgtg cggcgtgagt cagggcgttc aacgcggcgg
    20281 ttacggcggc atcatcacgg gtggcgcgaa tgcgttccag cgaagcaatt tgctcgttac
    20341 gcaccatcac gttgtcgatc tcaagtacat cggtttcgtc ttcgtgatcc agtttgtact
    20401 tgttgacacc aacgatgaca cgcttgccct ggtcgatcag cgactgttcg cgcgctgagg
    20461 cctcttcgat cattcgtttt ggcagacctg cttcgatcgc tttcgccatg ccaccggctt
    20521 cgtcgatctg ttggataata gctctggctt gtttgacgat ttgatcggtc agcgactcaa
    20581 tgtaatagga tccggccagt ggatcgacgg tgcggcagag ttctgattct tcctggatga
    20641 tgatctgggt gttgcgggca atgcgtgctg agaaatcggt aggcaaacca agcgcttcgt
    20701 caaaggcgtt ggtatgcagt gactgagtac cgcccagcgt cgcagccagc gcttcaatgg
    20761 tggtgcggat aacgttgtta tacggatcct gttcagtcag gctccagcct gaggtctggc
    20821 agtgggtacg cagcgccagt gatttcgggt cctgtgcgcc aaatccactg acctcttcgc
    20881 tccataaata acgtgccgca cgcaacatgg cgacgttcat aaacagatcc atgccgatgc
    20941 cgaagaagaa cgacaggcga ggagcgaagt catcaatttt cagtccggca gagattgctg
    21001 ctttgatgta ctcaatccca tcagcgagcg taaatgctac ctgctgcacg cagttggcac
    21061 ccgcttcacc catgtggtaa ccgctgatac tgatggtatt aaatcgcggc atgttgccgg
    21121 aacaccaggc gatgatgtcg gcgataatgc gcattgacgg ttttggcggg taaatatagg
    21181 tgttgcggca gaggtactct ttgagaatat cgttttgaat ggtgccggtc agtttatcag
    21241 gtgtaacacc ttgctcttct gcggcgacga tataaaacgc cagtactggt agcactgcgc
    21301 cattcatggt catcgaaacc gacattttat ccagcgggat ctggtcgaac aggactttca
    21361 tatcttccac ggtgtcgata gcgacgcccg ctttgccgac gtcacccgcc acgcgtgggt
    21421 tatcggagtc gtagccacgg tgggtggcaa ggtcaaacgc aacggaaaga cctttttgcc
    21481 cggcggccag gttacggcga taaaaagcgt tggactcttt tgctgttgaa aaaccagcat
    21541 actgacggat ggtccacggt tgggcggtat acatagtggc acgcgggcca cgaacgtagg
    21601 gcggcaaacc aggaagggta cctgtcacct ccagattatc gagatcggct tcggtataca
    21661 gcggcttgat ggcgatccct tccgcggttt gctgaaccag cgagtcgaca gttttctccc
    21721 gacggctcaa ttccttgttg gcaagctgtt gccactcctg ctcgttagac attatttgct
    21781 ccgtaaaacg caatcaaaat gagggtattt gcgcttacgg tgagcttttt acgtgggttt
    21841 gtcgccagca aaaaaaatgc attcagacgt cgttttttgc cgcctgagat ttgtctattt
    21901 tgtgatgtga ttcaaggaat acaaaaagaa gtgaggacaa cgcctgatat gtgctatcag
    21961 gcattatttg atggattaat cctgacgaag gactttgtga ccataatcga gtaacgcatc
    22021 agtgacttta cgcatcatgc ggctttcagg agcaaagcgg tgccagtaga gcatccgtcg
    22081 ttgaaatagc ccaggcgtta agtcaatcag ttcaccgctg gccagctctt tctcgatttg
    22141 caggtgcggg atcatacagc aggtggtgcc ctggcgagca agttgtacga acgcttctga
    22201 agaattaacg atatggcagg gcacgctgcc tggaggcaga tcgaagtttt gctgcaaaaa
    22261 ggcctggtgc atatcgtcaa gatggtcaaa cgcgaccact ggcgctttca gtaatgccga
    22321 acgcgttacg ccgttaggga aatatttttc ggcaaagggt tttgagctga cgaacagata
    22381 gtcgagcgca ccaagtttat cgacaagaca actcggcagc gcctgatgtt gaatactcac
    22441 cgcgccgacc acttcgccgc ggcgcagacg ttcctgagtg cgggtttcat cttctacctg
    22501 caagttgagg cggataggcg aatcagccaa cacaggagcc agtgcaggaa gcaaccacgt
    22561 cgccagactg tcggcgttga ccgccagtga aagcagcagc ggagtcgaac cggtttgttc
    22621 atcgcccagc cactcttctt ccagcaactc cacctggcgc agcagtgcca gcagtttttg
    22681 cccttgttcc gtcgggcgcg gcggtacggt acgcaccaac agcggctgcc cgaacatatt
    22741 ttccagttgc ttaatgcgct gtgagacggc tgattgtgta atgcacagct tttgtgccgc
    22801 gcgctcaaat cctcgttcac gtatcaccgc atccagtgcc tgtaatgttc tgtagtccgg
    22861 gcgtttcatt gctgttgtct gctcctggta aatgcttttt cctgcactat gacacaattt
    22921 tgtgtcaggt tgcatatacc gaagccgtcg gcgggtaaca tttgtattcg tggttaaacg
    22981 cgcagcttaa aagatcaggg atttgcgcta atgcatttcg ctcagcgagt tcgcgccctg
    23041 gttgtgttga atggcgtggc gttattgccc caatttgcct gtaaacaggg gcttgcgaac
    23101 ggtgaactgg tgcgcctgtt tgcaccgtgg agcggcatac ccagattgtt atatgcctta
    23161 tttgcggggc gaaaggggat gcctgccatt gcgcgatatt ttatggatga gttaaccacg
    23221 cggcttgcca acggggtctg aatcgctttt tttgtatata atgcgtgtga aatttcatac
    23281 cacaggcgaa acgatcatga cgcaggatga attgaaaaaa gcagtaggat gggcggcact
    23341 tcagtatgtt cagcccggca ccattgttgg tgtaggtaca ggttccaccg ccgcacactt
    23401 tattgacgcg ctcggtacaa tgaaaggcca gattgaaggg gccgtttcca gttcagatgc
    23461 ttccactgaa aaactgaaaa gcctcggcat tcacgttttt gatctcaacg aagtcgacag
    23521 ccttggcatc tacgttgatg gcgcagatga aatcaacggc cacatgcaaa tgatcaaagg
    23581 cggcggcgcg gcgctgaccc gtgaaaaaat cattgcttcg gttgcagaaa aatttatctg
    23641 tattgcagac gcttccaagc aggttgatat tctgggtaaa ttcccgctgc cagtagaagt
    23701 tatcccgatg gcacgtagtg cagtggcgcg ccagctggtg aaactgggcg gtcgtccgga
    23761 ataccgtcag ggcgtggtga ccgataatgg caacgtgatc ctcgacgtcc acggcatgga
    23821 aattcttgac ccgatagcga tggaaaacgc cataaatgcg attcctggcg tggtgactgt
    23881 tggcttgttt gctaaccgtg gcgcggacgt tgcgctgatt ggcacacctg acggtgtcaa
    23941 aaccattgtg aaatgatctg acgggggaac ctcccccgtt aaaaaaattc tcttcattaa
    24001 atttggtgac atgtgtcacg cttttaccag gcaattgtcg attgctctaa ataaatcctc
    24061 taaaccagca tattcatcca agaattacct ttgcgtgata tttcctcaac atcgcgacgc
    24121 aaacgttcat attgccgcaa tattattttt tgatatgttg aaaggcggat gcaaatccgc
    24181 acacaacatt tcaaaagaca ggattgggta aatggcaaag gtatcgctgg agaaagacaa
    24241 gattaagttt ctgctggtag aaggcgtgca ccaaaaggcg ctggaaagcc ttcgtgcagc
    24301 tggttacacc aacatcgaat ttcacaaagg cgcgctggat gatgaacaat taaaagaatc
    24361 catccgcgat gcccacttca tcggcctgcg atcccgtacc catctgactg aagacgtgat
    24421 caacgccgca gaaaaactgg tcgctattgg ctgtttctgt atcggaacaa accaggttga
    24481 tctggatgcg gcggcaaagc gcgggatccc ggtatttaac gcaccgttct caaatacgcg
    24541 ctctgttgcg gagctggtga ttggcgaact gctgctgcta ttgcgcggcg tgccggaagc
    24601 caatgctaaa gcgcaccgtg gcgtgtggaa caaactggcg gcgggttctt ttgaagcgcg
    24661 cggcaaaaag ctgggtatca tcggctacgg tcatattggt acgcaattgg gcattctggc
    24721 tgaatcgctg ggaatgtatg tttactttta tgatattgaa aacaaactgc cgctgggcaa
    24781 cgccactcag gtacagcatc tttctgacct gctgaatatg agcgatgtgg tgagtctgca
    24841 tgtaccagag aatccgtcca cccaaaatat gatgggcgcg aaagagattt cgctaatgaa
    24901 gcccggctcg ctgctgatta atgcctcgcg cggtactgtg gtggatattc cggcgctgtg
    24961 tgatgcgctg gcgagcaaac atctggcggg ggcggcaatc gacgtattcc cgacggaacc
    25021 ggcgaccaat agcgatccat ttacctctcc actgtgtgaa ttcgacaacg tccttctgac
    25081 gccacacatt ggcggttcga ctcaggaagc gcaggagaat atcggcctgg aagttgcggg
    25141 taaattgatc aagtattctg acaatggctc aacgctctct gcggtgaact tcccggaagt
    25201 ctcgctgcca ctgcatggtg gtcgtcgtct gatgcacatc cacgaaaacc gtccgggcgt
    25261 gctaactgcg ctgaacaaaa tcttcgccga gcagggcgtc aacatcgccg cgcaatatct
    25321 acaaacttcc gcccagatgg gttatgtagt tattgatatt gaagctgacg aagacgttgc
    25381 cgaaaaagcg ctgcaggcaa tgaaagctat tccgggtacc attcgcgccc gtctgctgta
    25441 ctaattcccc ttctctgaaa attaacgggc aggtcactga cttgcccgtt tttttatccc
    25501 ttctccacac cgttgccgcg ttgcgcattc tactttgcga gtctgctcca gaaaataaat
    25561 caaagtcaga tcaatgcgtt gtgtttattt aatggggatt tacgatggcg ggacagcatg
    25621 gggctgtaac ggtaaagccc tcaccgaagc gagggcttaa aggagaaggg ttatgatgcg
    25681 acttgtcatc atactgattg tactgttact gataagtttc ggagcttatt aacagtcagt
    25741 ctcaggggag gagtcatcct cccttaccct tactcactaa attaggtcaa agaatcaacg
    25801 atgtcaatca gggcgatgcg gggtgcatcg ccctgaaccg aactttttga tagccacttc
    25861 atcgcccacg tttactgccg taatagatcc tttttcccaa aaattatctc tgttttgcat
    25921 ggggtttttc gagcacgctc gaagaaaaga tttaaaggta gatcaatgcg ttgtggttat
    25981 ttaaagagag cttacgatag ctgggcagca tggggctgta acggtaaagc cctcaccgaa
    26041 gcgagggctt gaaggagaag ggttatgatg cgacttgcca tcatactgat tgtactgtta
    26101 ctcataagtt tcccagctta ttaacagtca gtcacagggg aggagcaatc ctcccttacc
    26161 cttactcact aaattaggtt aaagaatcaa cgatgtcaat cagggcgatg caggtgtatc
    26221 gcccttacca ctcccagact ttcgacggtg taaccaccgc aggaagaggg atatcccact
    26281 cttcaacggg gagtttttcc accaactgac aatcatgcgc ataacccacc ggttgcgttt
    26341 tatagtgctg ccagttttgt aaggtccgat cataaaaacc accgcccatt cccaggcgct
    26401 gaccgtactc atcaaaggcg accagcggtg tgatcagcac gtctaatcgg gaaaggggta
    26461 gcacgtcacg cacatccagt tttggctcat ggattttcaa cctgttcatc accagttcgc
    26521 tttgcggatg gtaattcagg aacagcaaat taccggcact aaagggatgc aaaactggaa
    26581 gataaacgcg cttaccggcg cgccagagtt gttctatgag tggctgggtg tcgagttcgc
    26641 catcaaaaga gaggaataca gcgaccgtat gtgccatcac caccggggga taagtcatca
    26701 tccgggtagc ggcttgttga cccatttctt gctgttgttc cggcgttaac gcacgacgac
    26761 gttgccgaat cattttgcgg atttcttgtc gggataatgt cagtgggagt tctggtagtt
    26821 gcgtcatggc tggtgccaga taagaaggga atctccgaga tgccgccgca ggctgtaacc
    26881 cttgaaccct tggttcaagg tgaatgtgtc gtcgcagttt taaggcttct cggacggacc
    26941 gagcatgctc accaaccgcg gagcgccaca ttcttgtggt atgaaatatc ggctcagggg
    27001 actggcccgc ttgcgaacat ctcagagaaa ttttgtcttc acggttactc taccacagta
    27061 aaccgaaaag tgtcattcaa agttttggtt agttttttcg gtgatgcgac cttgttcaag
    27121 taacgcttgt tctatggtct gctgcagcat ccgaatacgc tgttccatac ttgccgcgta
    27181 gtcacgagtc tttgcttttt cttgcgctaa ctcatagctg atattcaatg cggcaatgaa
    27241 gaccaactgt tcagtatttg tgactctagt gcgttctttc agatcttgca accgttggtt
    27301 cagatcgtcc gctgcctgat tcaacgcatc cctttggtca ggcgggcagt tcacacgcag
    27361 tgaacggcca aaaatttgga tatcgacggg ttgtgcagac atgccacctt cctgctgatt
    27421 gactgcgctg ccttcgtctt tagacccatg gtctgcgaag gggcgacact atagctaccc
    27481 tgatgagaag agacaagccc ttttctggtc caccagggcc caaagtggta gcatatcatg
    27541 aatattcctc cctttgacga cgaatgctta tgtctataca gaacgaaatg cctggttaca
    27601 acgaaatgaa ccagtatctg aaccaacaag ggacgggtct gacccctgct gagatgcatg
    27661 gtttaatcag tgggatgata tgtggcggta acgatgacag ctcatggcta ccgctacttc
    27721 acgacctgac gaacgaaggc atggctttcg gtcatgagct ggcacaggca ctgcgtaaaa
    27781 tgcactctgc caccagcgat gccctgcagg atgacggctt cctttttcag ctttatctgc
    27841 ctgatggcga tgatgtcagc gttttcgatc gggctgatgc attggcaggt tgggtcaatc
    27901 acttcctgct tggtcttggc gttacgcaac cgaagctgga taaagtgacc ggcgaaaccg
    27961 gtgaagctat cgacgatctg cgtaacattg cgcaactggg ttacgacgaa gacgaagatc
    28021 aggaagagct tgaaatgtcg cttgaagaga tcatcgaata cgttcgtgtt gccgcgctgt
    28081 tatgccacga cacctttact catccgcaac cgaccgcgcc agaagtacaa aaaccgactc
    28141 tacactaaaa acaaaaaacg taaggagagt gttatgagtg agatatcccg gcaagagttt
    28201 cagcgtcgcc gtcaggccct ggtggagcaa atgcaacccg gcagcgccgc gctgattttt
    28261 gctgcaccag aagtaacacg tagcgccgac agcgaatacc cctatcgtca gaacagtgac
    28321 ttctggtact tcaccggctt taacgaaccg gaagcggtgc tggtgctgat taaaagcgat
    28381 gacactcata accacagcgt tctgtttaac cgcgttcgcg acctgacggc ggagatctgg
    28441 tttggccgtc gcttaggcca ggatgccgcg ccagagaaac tgggcgttga ccgcgcactg
    28501 gcattcagcg aaatcaatca gcaactttat caactactta acggcctgga tgtggtttac
    28561 catgcccagg gcgaatatgc atatgctgat gaaatcgtga acagtgcgct ggaaaaactg
    28621 cgtaaaggtt cgcggcaaaa tctcaccgca ccggcaacga tgatcgactg gcgtcctgtt
    28681 gttcatgaaa tgcgcctgtt caaatcgcca gaagagattg ccgtactccg ccgcgcggga
    28741 gaaatcaccg ccatggcaca tacacgggcg atggaaaaat gccgtccggg aatgttcgag
    28801 taccatctgg aaggcgaaat tcaccacgaa tttaaccgcc acggtgcgcg ctatccgtcc
    28861 tataacacca ttgtcggcag cggtgaaaac ggctgcattc tgcactacac cgaaaacgag
    28921 agtgaactgc gcgacggtga cctggtgttg attgacgcgg gttgtgaata caaaggttac
    28981 gcgggcgata ttacccgcac cttcccggtc aacggcaaat tcacccaggc ccagcgtgaa
    29041 atctacgaca ttgtgctgga gtctctcgaa accagcctgc gcctgtatcg tccgggaact
    29101 tccattctgg aagtcactgg tgaagtggtg cgcatcatgg ttagcggcct ggtaaaactc
    29161 ggcatcctga aaggtgatgt tgatgaactg atcgctcaga acgcccatcg tcctttcttt
    29221 atgcatggcc ttagccactg gttaggactg gatgtccatg acgttggcgt ttatggtcag
    29281 gatcgctcgc gcattctgga accggggatg gtactgaccg tagagcctgg tctgtatatc
    29341 gcgccggatg cggatgtgcc agaacaatat cgcggtatcg gcattcgtat tgaagacgac
    29401 attgtgatta ccgaaaccgg caacgaaaac ctcaccgcca gcgtggtgaa aaagccggaa
    29461 gaaatcgaag cgttgatggc agctgcgaga aagcaatgag cgtaatcatc gtcggtggcg
    29521 gcatggcggg cgcgacgctg gcgctggcta tttcccggtt aagtcacggg gcgctgccgg
    29581 tacatttgat tgaagcgacc gcgccagagt cacatgctca tccgggcttt gatggacgag
    29641 cgatagcgct ggcggcgggt acctgtcagc aactggcgcg catcggcgtc tggcaatctc
    29701 tggcggatta cgcaaccgcc atcactaccg tgcatgtcag cgatcgtggt cacgctggat
    29761 ttgccaccct cgccgcagaa gattaccaac tggcggtgct gggacaggtt gtcgaattgc
    29821 acaatgtcgg gcaacggctg tttgcattgc tgcgtaaagc acctggcgta acgctgcatt
    29881 gccctgatcg cgtggctaac gttgcccgta ctcagagtca cgttgaagtg acgctggaga
    29941 gtggcgagac gctgacgggc cgcgtgctgg tagcagctga tggcacccat tcagcgttag
    30001 ccaccgcgtg cggcgttgac tggcagcagg agccttacga acaactggcc gtgattgcca
    30061 acgttgcgac ttccgttgcg cacgaaggcc gcgcttttga acgcttcacg caacatggcc
    30121 cgctggcgat gttgccgatg tctgacggac gctgttcgct ggtctggtgt catccactgg
    30181 aacggcgcga agaggtgctg tcgtggagcg acgagaagtt ttgccgtgaa ctccagtcgg
    30241 cctttggctg gcgacttgga aaaattaccc acgccggtaa acgcagtgct tatccgctgg
    30301 cgttaacccg cgccgccaga gctattaccc atcgtaccgt gctggtgggc aatgcggcgc
    30361 aaactctgca cccgattgcc gggcaagggt ttaacctcgg tatgcgagat gtcatgagcc
    30421 ttgcggaaac cctgactcag gcgcatgagc gcggagaaga catcggtgat tacggcatat
    30481 tgtgccgcta tcagcagcgt cgacagagcg atcgcgaagc aaccatcggc gtcacggaca
    30541 gcctggtaca tctttttgcc aaccgttggg caccgctggt tgtcgggcgc aacatcggac
    30601 tgatgacgat ggaattattc accccggcac gcgatgtgct ggcgcagcgc accctcggtt
    30661 gggtggcgcg ttgaaggcgg ttttaaggaa taaacaatgc aaagtgttga tgtagccatt
    30721 gttggtggcg gcatggtggg gctggcggtt gcctgcggct tacaggggag cggcttacgc
    30781 gttgccgtac tggagcagcg cgtaccggaa cctctggcgg cggatgcacc accacaactg
    30841 cgcgtttcgg ctatcaatgc cgccagcgaa aaattactca cccgtcttgg cgtctggcag
    30901 gacattctct ctcgtagggc cagttgttat cacggtatgg aagtgtggga caaagacagt
    30961 tttggtcaca tttcgtttga cgatcaaagc atgggctata gccatcttgg gcatatcgtt
    31021 gaaaactcag tgattcacta cgcgctgtgg aacaaagcgc agcagtcgtc agataccact
    31081 ctgttggccc ccgcagaatt acagcaggtc gcctggggag aaaacgaaac cttcctgacg
    31141 ctgaaagatg gcagtatgtt aacggcgcgt ctggtgattg gcgcggacgg cgctaattcc
    31201 tggttgcgca acaaagccga tattccgctg actttctggg attatcagca tcacgcgctg
    31261 gtagcgacca ttcgcacgga agaaccgcat gatgcggtgg cgcggcaggt tttccatggc
    31321 gaaggcattc tggccttttt accgcttagc gatccgcatc tttgctcgat tgtctggtca
    31381 ctgtcgccag aggaagcgca gcgtatgcag caggcagggg aagacgaatt taatcgcgcg
    31441 ttaaatatcg cttttgataa tcgcctgggc ttatgcaagg ttgagagcgc gcgtcaggtg
    31501 ttcccactga cggggcgtta tgcgcgccag tttgccgcgc accgtctggc gctggtgggc
    31561 gacgccgcgc ataccattca cccgctggcg gggcaggggg taaatctcgg ctttatggat
    31621 gctgcagagc tggttgctga actgaaacgg ttgcatcgtc agggtaaaga catcgggcag
    31681 tacatttatc tgcgtcgcta tgagcgtagc cgcaagcaca gtgcggcgat gatgctggct
    31741 ggtatgcagg gattccgcga tctgttttcc ggtaccaatc cggcgaaaaa actgctgcgt
    31801 gatattggtc tgaaactggc cgacacgctt cctggcgtta aaccacaact tatccgccag
    31861 gcaatgggat taaacgattt gcctgaatgg ctgcgttaaa aatttctctt ctgctgttta
    31921 tttgataccc atcacacttt catctcccgg ttttttcgcc gggagatttt cctcatttga
    31981 aataaactaa tttcacctcc gttttcgcat tatattttct aatgtcacga atttttgatt
    32041 tagtgttttt tgacattttt tcagctctta atattgtctt attcaaatta actttctcat
    32101 cacatcatct ttatataaaa acttgtgtat tttttggttt tttattctgt tgcgattttg
    32161 gcgtttttta accataagct aatgtgatga tcaattttac cttatggtta acagtctgtt
    32221 tcggtggtaa gttcaggcaa aagagaacga ttgcgttggg gaccgggagt ggctccgatg
    32281 ctgggtttcg tggtgataat ttcactatga aaaagttgtc agccccgctt attcaatgag
    32341 gacaagatgg cacaacagac tcctttgtac gaacaacata cgctttgcgg cgctcgcatg
    32401 gtggatttcc acggctggat gatgccgctg cattacggtt cgcaaatcga cgaacatcat
    32461 gcggtacgta ccgatgccgg aatgtttgat gtgtcacata tgaccatcgt cgatcttcgc
    32521 ggcagccgca cccgggagtt tctgcgttat ctgctggcga acgatgtggc gaagctcacc
    32581 aaaagcggca aagcccttta ctcggggatg ttgaatgcct ctggcggtgt gatagatgat
    32641 ctcatcgtct actactttac tgaagatttc ttccgcctcg ttgttaactc cgccacccgc
    32701 gaaaaagacc tctcctggat tacccaacac gctgaacctt tcggcatcga aattaccgtt
    32761 cgtgatgacc tttccatgat tgccgtacaa gggccgaatg cgcaggcaaa agctgccaca
    32821 ctgtttaatg atgctcagcg tcaggcggtg gaagggatga aaccgttctt tggcgtgcag
    32881 gcgggcgatc tgtttattgc caccactggt tataccggtg aagcgggcta tgaaattgcg
    32941 ctgcccaatg aaaaagcggc ggatttctgg cgcgcgctgg tggaagcggg cgttaagcca
    33001 tgcggcctgg gcgcgcgtga cacgctgcgt ctggaagcgg gcatgaatct ttatggtcag
    33061 gagatggacg aaactatttc tcctttagcc gccaacatgg gctggactat cgcctgggaa
    33121 ccggcagatc gtgacttcat cggtcgtgaa gccctggaag tgcagcgtga gcatggcaca
    33181 gaaaaactgg ttggtctggt gatgaccgaa aaaggcgtgc tgcgtaatga actgccggta
    33241 cgttttaccg atgcgcaggg caaccagcat gaaggcatta tcaccagcgg gactttctcc
    33301 ccgacgctgg gttacagcat tgcgctggca cgcgtgccgg aaggtattgg cgaaacggcg
    33361 attgtgcaaa ttcgcaaccg tgaaatgccg gttaaagtga cgaaacctgt ttttgtgcgt
    33421 aacggtaaag ccgtcgcgtg atttactttt ttggagattg attgatgagc aacgtaccag
    33481 cagaactgaa atacagcaaa gaacacgaat ggctgcgtaa agaagccgac ggcacttaca
    33541 ccgttggtat aaccgaacat gcccaggagc tgttaggcga tatggtgttt gttgacctgc
    33601 cggaagtggg cgcaacggtt agcgcgggcg atgactgcgc ggttgccgaa tcagtaaaag
    33661 cggcgtcaga catttatgcg ccagtaagcg gtgaaatcgt ggcggtaaac gacgcactga
    33721 gcgattcccc ggaactggtg aacagcgaac cgtatgcagg tggctggatc tttaaaatca
    33781 aagccagcga tgaaagcgaa ctggaatcac tgctggatgc gaccgcatac gaagcattgt
    33841 tagaagacga gtaacggctt tattcctctt ctgcgggaga ggatcagggt gaggatattt
    33901 acgcctcacc ctcactctct tcgcaaggag agaggttcac aattcactgc acgtttcagg
    33961 aaccatcgct catgacacag acgttaagcc agcttgaaaa cagcggcgct tttattgaac
    34021 gccatatcgg accggacgcc gcgcaacagc aagaaatgct gaatgccgtt ggtgcacaat
    34081 cgttaaacgc gctgaccggc cagattgtgc cgaaagatat tcagcttgcg accccgccgc
    34141 aggttggcgc accggcgacc gaatacgccg cactggcaga actcaaggct attgccagtc
    34201 gcaataaacg cttcacgtct tacatcggca tgggttacac cgccgtgcag ctaccgccgg
    34261 ttatcctgcg taacatgctg gaaaatccgg gctggtatac cgcgtacact ccgtatcaac
    34321 ctgaagtctc ccagggccgc cttgaagcac tgctcaactt ccagcaggta acgctggatt
    34381 tgactggact ggatatggcc tctgcttctc ttctggacga ggccaccgct gccgccgaag
    34441 caatggcgat ggcgaaacgc gtcagcaaac tgaaaaatgc caaccgcttc ttcgtggctt
    34501 ccgatgtgca tccgcaaacg ctggatgtgg tccgtactcg tgccgaaacc tttggttttg
    34561 aagtgattgt cgatgacgcg caaaaagtgc tcgaccatca ggacgtcttc ggcgtgctgt
    34621 tacagcaggt aggcactacc ggtgaaattc acgactacac tgcgcttatt agcgaactga
    34681 aatcacgcaa aattgtggtc agcgttgccg ccgatattat ggcgctggtg ctgttaactg
    34741 cgccgggtaa acagggcgcg gatattgttt ttggttcggc gcaacgcttc ggcgtgccga
    34801 tgggctacgg tggcccacac gcggcattct ttgcggcgaa agatgaatac aaacgctcaa
    34861 tgccgggccg tattatcggt gtatcgaaag atgcagctgg caataccgcg ctgcgcatgg
    34921 cgatgcagac tcgcgagcaa catatccgcc gtgagaaagc gaactccaac atttgtactt
    34981 cccaggtact gctggcaaac atcgccagcc tgtatgccgt ttatcacggc ccggttggcc
    35041 tgaaacgtat cgctaaccgc attcaccgtt tgaccgatat cctggcggca ggcctgcaac
    35101 aaaaaggtct gaagctgcgc catgcgcact atttcgacac tctgtgtgtg gaagtggccg
    35161 acaaggcggg cgtactggcg cgtgccgaag cggctgaaat caacctgcgt agcgatatcc
    35221 tgaacgcggt tgggatcacc cttgatgaaa ccaccacgcg tgaaaacgta atgcagcttt
    35281 tcagcgtgct gctgggcgat aaccacgggc tggagatcga cacgctggac aaagacgtgg
    35341 ctcacgacag ccgctctatc cagcctgcga tgctgcgcga cgacgaaatc ctcacccatc
    35401 cggtgtttaa tcgctaccac agcgaaaccg aaatgatgcg ctatatgcac tcgctggagc
    35461 gtaaagatct ggcgctgaat caggcgatga tcccgctggg ttcctgcacc atgaaactga
    35521 acgccgccgc cgagatgatc ccaatcacct ggccggaatt tgccgaactg cacccgtttt
    35581 gtccgccgga acaggctgaa ggttatcagc agatgattgc gcagctggct gactggctgg
    35641 tgaaactgac cggttacgac gccgtttgta tgcagccgaa ctccggcgca cagggcgaat
    35701 acgcgggcct gctggcgatt cgtcattatc atgaaagccg caacgaaggg catcgcgata
    35761 tctgcctgat cccggcttct gcgcacggaa ctaaccccgc ttctgcacat atggcaggaa
    35821 tgcaggtggt ggttgtggcg tgtgataaaa acggcaacat cgatctgact gatctgcgcg
    35881 cgaaagcgga acaggcgggc gataacctct cctgtatcat ggtgacttat ccttctaccc
    35941 acggcgtgta tgaagaaacg atccgtgaag tgtgtgaagt cgtgcatcag ttcggcggtc
    36001 aggtttacct tgatggcgcg aacatgaacg cccaggttgg catcacctcg ccgggcttta
    36061 ttggcgcgga cgtttcgcac ctcaacctgc ataaaacttt ctgcattccg cacggcggtg
    36121 gtggtccggg tatgggaccg atcggtgtga aagcgcatct ggcaccgttt gaaccgggtc
    36181 atagcgtggt gcaaatcgaa ggcatgttaa cccgtcaggg cgcagtttct gcggcaccgt
    36241 tcggtggcgc ctctatcctg ccaatcagct ggatgtacat ccgcatgatg ggcgcagaag
    36301 ggctgaaaaa agcaagccag gtggcaatcc tcaacgccaa ctatattgcc agccgcctgc
    36361 aggatgcctt cctggtgctg tataccggtc gcgacggtcg cgtggcgcac gaatgtattc
    36421 tcgatattcg cccgctgaaa gaagaaaccg gcatcagcga gctggatatt gccaagcgcc
    36481 tgatcgacta cggtttccac gcgccgacga tgtcgttccc ggtggcgggt acgctgatgg
    36541 ttgaaccgac tgaatctgaa agcaaagtgg aactggatcg ctttatcgac gcgatgctgg
    36601 ctatccgcgc agaaatcgac caggttaaag ccggtgtctg gccgctggaa gataacccgc
    36661 tggtgaacgc gccgcacatt cagaacgaac tggtcgccga gtgggcgcat ccgtacagcc
    36721 gtgaagttgc agtattcccg gcaggtgtgg cagacaaata ctggccgacg gtgaaacgtc
    36781 tggatgatgt ttacggcgac cgtaacctgt tctgctcctg cgtaccgatt agcgaatacc
    36841 agtaattcac tgattcgact atcttctaaa ggcgcttcgg cgccttttta gtcagatgac
    36901 aaagtacaaa agcgctcaga cagtcccctc acccctttgg ggagagggtt agggtgaggg
    36961 gaacaggccg gcactggcgc gaatatttac cctcaccccg gccctctccc tgaaagggcg
    37021 aggggaaaag cgtgccaaca ttgaagattg agccagtttg ttagcaatct caaagatacg
    37081 tcaacgaatt aatttttctc ggaaaaacaa atggctatag cacttgtgac cggtggcagt
    37141 cgcggcatcg ggcgggcaac tgcattactg ttggcgaaag aagagtatac ggtggcggtt
    37201 aattatcaac aaaacctcca tgcggcgcag gaagtggtga acttaatcac gcaagccggt
    37261 ggcaaggcat tcgtgctcca ggcggatatc agcgacgaaa atcaggtcat cgcaatgttt
    37321 acagcaatcg atcagcacga tgaaccgcta gcagcgctgg tcaataacgc cgggatcttg
    37381 tttacccagt gcaccgttga aaaccttacc gcagagcgaa tcaaccgagt actttccacc
    37441 aacgtgacgg gatattttct ctgctgccgc gaggcggtaa aacgcatggc gcttaaaaat
    37501 ggtggcagtg gcggcgctat cgtcaatgtc tcttcggtgg cctcacggtt gggttcgcca
    37561 ggggaatatg ttgattacgc ggcatcgaaa ggggcgattg atacgttaac caccggacta
    37621 tcgctggaag tcgccgcgca ggggatccgc gttaactgcg tgcggccagg gtttatttat
    37681 accgaaatgc acgccagcgg cggcgagcct ggacgcgtcg atcgcgttaa gtcgaacatc
    37741 cccatgcagc gtggtggaca ggcagaagag gtcgcgcagg ccattgtctg gctactaagt
    37801 gataaagcct cttacgtcac gggaagtttt atcgatttgg cgggcgggaa ataaaacagg
    37861 gaagttgtct gaccggatgc aacaagtatt gcatccggta cttcatcgac ttaaagcttc
    37921 tcgccgttgc tggcaatcac ctctttgtac cagttaaagc tcttcttacg tgaacgcgac
    37981 atatcgccag taccgtcgtc atgtttattc acatagataa agccgtagcg tttgctgtac
    38041 tgcccggtag tgaatgacac gcagtcgatg cagccccacg gtgtgtagcc catcagatcc
    38101 acaccatcgt aagtcactgc ttttttcatc tcttcaatat gggcgcgcag gtagtcaatg
    38161 cggtagtcgt cgttgatgct gccatcttct tccactttgt cgtaagcgcc aaaaccgttt
    38221 tcgacaataa acagcggcct ctgataacgc tcatacagtt cgcaaagggc atagcgcaga
    38281 cctactggat caatctgcca gccccagtca gacgctttaa catacgggtt cggtacgctg
    38341 ccttcaaaac cagagatcgc atcgcctgtg ccgccttcgg ccttcactgc gttggtcatg
    38401 taatagctga aaccaagata atcgcaggtg ccttcacgca gtacatcgag atcgccgtct
    38461 tccattttga tgttaaatcc gcgacgctcc cactcgttca acacatagga cgggtaatag
    38521 ccgcgtagct gcacatcggt aaagacgtag cgttcgcgca tcgactcctg agcgaacatc
    38581 acatcgtccg ggttacagca gtagggatag agcggcacca tcgccagcat acagccgact
    38641 ttcatctccg ggttaatgcg acgcgcagct ttcaccgcca gggcgctggc gacaaactgg
    38701 tgatgcagca cctgatacat cgtctcttcc gggttctcat gctcggtata aaccacgccg
    38761 gagcagcagt aaccgaacag cggtgcacgc cagttacgct ggttgttaat ttcgttgaag
    38821 gtcatccagt atttgacttt atgcttatag cgttcaaaca ccacttccgc gaaacggaca
    38881 aagaaatcaa ccactttacg gttggtccag ctaccgtatt gctgcaccag atgcagcggc
    38941 atttcaaagt gggagagggt gatcaccggt tcgatgttgt atttcagcag ttcatcgaac
    39001 atatcatcgt agaacttcag cccttcttcg tttggctgag cttcatcacc ttttggaaaa
    39061 atacgcgtcc aggcgatgga agtccgaaaa catttgaagc ccatttcggc aaatagcttg
    39121 atgtcttcct tgtagtgacc ataaaaatca acggcttcat ggtttggata gtatttgcct
    39181 ggcaagactt ctttggtgat ttcgcgcggc acgccgtgtg cgccaccggt cagaacgtca
    39241 caaatgctcg gccctttgcc gcctttgttc cagccgcctt cgacctgatg agcggcaact
    39301 gcgccgcccc ataagaaatc tttcggtaag gtgagttttt tcactatcat aggctccaga
    39361 tgagtcaatt cttacaggaa atagtaccat gcagccaaac gacatcactt tttttcaacg
    39421 tttccaggat gacattctgg ctgggcgtaa aaccatcacc atccgcgacg agtctgaatc
    39481 gcacttcaaa acgggtgatg tgcttcgtgt cgggcgtttt gaagatgacg gttatttttg
    39541 cacgattgaa gtcaccgcaa cctcaaccgt aacgctggat acgctgacgg aaaaacatgc
    39601 agagcaggaa aatatgaccc tgactgaact gataaaggtc attgccgaca tctatcccgg
    39661 tcagacacag ttttatgtga ttgaatttaa atgtctttaa attgaactca cgcctgttag
    39721 ttgaaatttc gcatttaatt tcatgatttt taggagatta gtctcaaatc agaatttatt
    39781 ttagctaaca ggtgttcact ggaactattc tcagttacgc tggagaggta tacatgtgcg
    39841 tgtacgtttc tccggagtaa gttatggttc agaagcccct cattaagcag ggatattcgc
    39901 tggcagagga aattgccaac agcgtcagtc acggcattgg gttggtgttt ggtatcgttg
    39961 ggctggtgtt gctactggtt caggcggtgg atctcaatgc cagcgccaca gcgataacca
    40021 gttacagcct ctatggcggc agtatgatcc tgctgttcct cgcttcgacg ctctatcacg
    40081 ccattcctca tcaacgggca aaaatgtggc tgaagaaatt tgaccattgc gctatttacc
    40141 tgttgattgc cggaacctac acgccgtttt tgctggtggg gctggattct ccgttagcgc
    40201 gcgggttgat gattgttatc tggagcctgg cattgctggg tattctgttt aaactgacca
    40261 tcgcgcaccg attcaaaatt ttatctctgg tgacctatct ggcgatgggc tggctgtcgc
    40321 tggtggtaat ttatgaaatg gcagttaagc tcgcggcggg cagcgttacc ttactggcgg
    40381 taggcggcgt ggtttattcg ctcggggtga ttttctacgt ctgcaaacgc attccataca
    40441 accatgccat ctggcacggc ttcgtgctcg gcggtagtgt gtgccacttt ctggcgatct
    40501 atttgtatat tgggcaggcg taatgggtat gcctttgtag gcctgataag acgcgccagc
    40561 gtcgcatcag gcgataaatg cctgatgcga taaagagatt tactcttcga gcgaatacgg
    40621 cagcggctcg atatgcagtg tattcgcatc gtcgcgtacg cggaagatgc tatccggttc
    40681 catatcgtta ttcatgacga cctgtaccac gacctgacca tcttccagtt ttaccgcagc
    40741 cagcaccgta ccggtacgac gccagttctc gcccattttc agctctaagt cttcaccagc
    40801 ttccggcagt cggctggcgc tacctgccag caaccagagc gcacgtttat tggcaccacg
    40861 gaattttgct cgcgccacca tctcttgtcc ggtatagcag ccttttttaa agctgatacc
    40921 gcccagcgcc tggaggttgg tcgcctgtgg gataaactgc ccgctgttgg cggcatcaat
    40981 caccgggaaa cccgcttcaa tgtttaatgc cagccactgt tggctattgt tcagttccgc
    41041 ttcaccgcgc agtttatcgg tcagcatatt agcagtagct tcatcggtta cgatcaggaa
    41101 acgttctgcc gggtgttcaa accatagcaa agtggtcgcg ccttctttaa ctacctgttt
    41161 ttctttcgaa ggcagttcac taaagagatt tgccagcgcg gcgcgcgcct gaaaaccggc
    41221 aacaccaagc agcacacgct cgtcgtctgg cgcgatggtc actttagaga ataccgcata
    41281 ttttttcagt tcagtcagct gcggttcacg cacgctgcgc cgttcaatcc atgcaaagcc
    41341 atcgccgtcg cggaacagac gtaaattgct ccacatttta cctttggcgt cgcaatgggc
    41401 ggcgagcagg tgctgatctt ctgccatctg gctgacatct gctgtcacct gaccctgcat
    41461 atatttttcg ctgtccgcgc cagtaatggt ggcaagcgcc cagtcatcaa gcgtcatcag
    41521 cgtcagcggt aaacgggcag aagccgtagg ctgacgggga ggaaaaggtg taaaagccat
    41581 aatagagtcc tgagttgctt aacggcgtat taatgactaa tggtaaaaga gctatgaggc
    41641 aatgcaagtg ctttcactac gcgatctgtg ccttctgcga ggcgacttcc aaaaaaatga
    41701 agccattgaa ttgttgtgaa ttaatttcgc gatcgcgatg gtgtttactg atattcccgt
    41761 aaaagaaggc gaaaaacacg ttacaatagc ggggtactgg attttcgtga gacaggaaga
    41821 agatggacat taacaacaaa gcccgcattc attgggcatg ccgccgtggt atgcgcgaac
    41881 tcgatatttc aatcatgccg tttttcgaac atgagtacga cagcttaagc gatgacgaaa
    41941 aacgcatctt tattcgtctg ctggaatgtg acgatccgga cctgtttaac tggctgatga
    42001 atcacggtaa accggccgat gcagaactgg aaatgatggt ccgactcatc cagacacgga
    42061 accgggaacg tggtcctgtg gcaatctgat ttgcgcgtct cctggcgcgc acagtggctt
    42121 tccttgctga ttcatgggct ggttgccgct gttattttac tcatgccctg gccactcagt
    42181 tacaccccgt tatggatggt gttactttcg ctggtggtgt ttgattgcgt tcgcagccag
    42241 cggcgtatta atgctcgcca gggggaaatt cgcttgttga tggacgggcg tttgcgttgg
    42301 caagggcagg agtggagcat cgtcaaagca ccgtggatga ttaagagcgg catgatgctg
    42361 cgtttacgtt ctgatggcgg taaacggcaa catttatggc tggcagccga cagcatggac
    42421 gaagccgaat ggcgggattt acggcggatt ttgttgcaac aagagacgca aagataaaga
    42481 tgacggcaaa gcatatgcgc taagccgtca gtgagatcag gcgtaatgct ctgccatttc
    42541 gttgaggatt tgctcgcacc agctctgaat acgctcgtcg ctaaggtcat actggttagt
    42601 ttcatccagc gccagaccca cgaacagttg cccgtcagca atcaccggtt tcgggctggt
    42661 aaattcatat ccttccgttg gccagtagcc gacgaacttc acgcctttgg tcgagagttt
    42721 gtcatgcagc ataccgagcg catcgaggaa ccactcgccg tatcccagtt gatcgccaag
    42781 cccatacagc gcaacaattt taccttcaag gttcaggtcg tcgagctgat cccagacggc
    42841 ttcccagtct tcttggattt caccaaaatc ccaggtcggg atacccagaa tgagcacatc
    42901 gtactgctcc attaatttcg gggagtcgtc cttgaggtta tgtaaggtca ccagttctgg
    42961 gccgataata tcgcggattt tttctgccgc catttcggtg taacaggtgc tggaaccgta
    43021 aaaaagaccc atattcataa cgtaagcttc tcgattctgc tgtggctttt gcggctagtg
    43081 tatcagaatc gctttagtca cggcacaata aggcataatg gatcaaatga gtgaccataa
    43141 ggggcgcaag tgaaacagga tctggcacgc atcgagcagt ttcttgatgc tctgtggctg
    43201 gaaaaaaatc tggctgaaaa tacgttgaac gcttaccgtc gcgatctgtc aatgatggtg
    43261 gagtggttgc atcaccgcgg gttgacgctg gcgacggcgc aaagtgacga tttgcaggca
    43321 ttactggcag aacggctgga gggcgggtat aaagccacca gctcagcgcg tttgctgagt
    43381 gcagtgcgcc gattgttcca gtatctttat cgcgaaaagt ttcgtgaaga cgatcccagt
    43441 gcgcatctcg cttcaccgaa attgccccag cgtttgccaa aagatttaag cgaagcgcag
    43501 gttgaacgtt tattacaggc accattaatt gatcagccac tggagctacg cgataaagcc
    43561 atgattgaag tgttgtatgc taccgggctg cgtgtctctg aactggtcgg actgacaatg
    43621 agtgatatca gcctgcgtca gggcgtagtg cgggtcattg gtaaaggcaa caaagagcgc
    43681 ctggtaccgt taggtgaaga ggcggtttac tggctggaaa cctatctgga acatgggcgt
    43741 ccgtggctgc tgaatggtgt gtcaattgac gtgttgtttc ccagccagcg tgcgcagcag
    43801 atgacgcgac agaccttctg gcaccgcatt aaacattatg ctgtgctggc gggtatcgac
    43861 agcgaaaagc tgtcaccgca tgtgttgcgt cacgcttttg ccacccattt attaaatcat
    43921 ggtgcggatt tacgcgtggt gcagatgcta ctgggccaca gcgacctctc caccacgcaa
    43981 atttatacgc atgtcgctac cgagcgtctg cggcaacttc atcaacagca tcacccgcgg
    44041 gcgtgatgtc tgaaaagaac gggaagattt atgaagaaag gttttatgtt gtttactttg
    44101 ttagcggcgt tttcaggttt tgctcaggct gatgacgcgg caattcaaca aacgttagcc
    44161 aaaatgggca tcaaaagcag cgatattcag cccgcgcccg tagctggcat gaagacagtt
    44221 ctgactaaca gcggcgtgtt gtacatcacc gatgatggta aacatatcat tcaggggcca
    44281 atgtatgacg ttagcggcac ggctccggtc aatgtcacca ataagatgct gttaaagcag
    44341 ttgaatgcgc ttgaaaaaga gatgatcgtt tataaagcgc cgcaggaaaa acacgtcatc
    44401 accgtgttta ctgatattac ctgtggttac tgccacaaac tgcatgagca aatggcagac
    44461 tataacgcgc tggggatcac cgtgcgttat cttgctttcc cgcgccaggg gctggacagc
    44521 gatgcagaga aagaaatgaa agctatctgg tgtgcgaaag ataaaaacaa agcgtttgat
    44581 gatgtgatgg caggtaaaag cgtcgcacca gccagctgcg acgtggatat tgccgaccat
    44641 tacgcacttg gcgtccagct tggcgttagc ggtactccgg cagttgtgct gagcaatggc
    44701 acacttgttc cgggttacca gccgccgaaa gagatgaaag aattcctcga cgaacaccaa
    44761 aaaatgacca gcggtaaata attcgcgtga aacaacagat acaacttcgt cgccgtgaag
    44821 tcgatgaaac ggcagacttg cccgctgaat tgcctccctt gctgcgccgt ttatacgcca
    44881 gccggggcgt gcgcagtgcg caagaactgg aacgcagtgt taaaggtatg ttgccctggc
    44941 agcaactgag cggcgtcgaa aaggccgttg agatccttta caacgctttt cgcgaaggaa
    45001 cgcggattat tgtggtcggc gattttgacg ccgacggcgc gaccagcacg gctctaagcg
    45061 tgctggcgat gcgctcgctt ggttgcagca atatcgacta tctggtacca aaccgtttcg
    45121 aagacggtta cggcttaagc ccggaagtag tcgatcaggc ccatgcccgt ggcgcgcagt
    45181 taattgtcac ggtggataac ggtatttcct cccatgcggg cgttgaacac gctcgctcgt
    45241 tgggcattcc ggttattgtt accgatcacc atttgccggg cgaaacatta cccgcagcgg
    45301 aagcgatcat taaccctaac ttgcgcgact gtaatttccc gtcgaaatca ctggcaggcg
    45361 tgggtgtggc gttttatctg atgctggcgc tgcgcacctt tttgcgcgat cagggctggt
    45421 ttgatgagcg tggcatcgca attcctaacc tggcagaact gctggatctg gtagcgctgg
    45481 ggacagtagc ggacgtcgtg ccgctggacg ctaataatcg cattctgacc tggcagggga
    45541 tgagtcgcat ccgtgccgga aagtgccgtc cggggattaa agcgctgctg gaagtggcaa
    45601 atcgcgatgc gcaaaaactc gccgccagcg atttaggttt tgcgctgggg ccacgtctca
    45661 atgctgccgg acgactggac gatatgtccg tcggtgtggc gctcttgctg tgcgacaaca
    45721 tcggcgaagc gcgcgtgctg gcaaatgaac tcgatgcgct aaaccagacg cgaaaagaga
    45781 tcgaacaagg aatgcaagtt gaagccctga ccctgtgcga gaaactggag cgaagtcgcg
    45841 acacgctacc cggcgggctg gcaatgtatc accccgaatg gcatcagggc gttgtcggta
    45901 ttctggcttc gcgcatcaaa gagcgttttc accgtccggt tatcgccttt gcgccagcag
    45961 gtgatggtac gctgaaaggt tcaggtcgct ccattcaggg gctgcatatg cgtgatgcac
    46021 tggagcgatt agacacactc taccctggca tgatactgaa gtttggcggt catgcgatgg
    46081 cggcgggttt gtcgctggaa gaggataaat tcgaactctt tcaacaacgg tttggcgaac
    46141 tggttacaga gtggctggac ccttcgctat tgcaaggcga agtggtgtca gacggtccgt
    46201 taagcccggc cgaaatgacc atggaagtgg cgcagttgct gcgcgatgct ggcccgtggg
    46261 ggcagatgtt cccggagccg ctgtttgacg gtcatttccg tctgctgcaa cagcggctgg
    46321 tgggcgaacg tcatttgaag gtgatggtcg aaccggtcgg cggcggtcca ctgctggatg
    46381 gtattgcttt taatgtcgat accgccctct ggccggataa cggcgtgcgt gaagtgcaac
    46441 tggcttataa gctcgatatc aacgagtttc gcggcaaccg cagcctgcaa attatcatcg
    46501 acaatatctg gccaatttag cgtcatcttc tctataaaaa agagcgtgga ttgggtacaa
    46561 tcccgctctt atcaccgcat tttgactagc tcaataaaag aaatcagacc atgtttgaaa
    46621 ttaatccggt aaataatcgc attcaggacc tcacggaacg ctccgacgtt cttagggggt
    46681 atctttgact acgacgccaa gaaagagcgt ctggaagaag taaacgccga gctggaacag
    46741 ccggatgtct ggaacgaacc cgaacgcgca caggcgctgg gtaaagagcg ttcctccctc
    46801 gaagccgttg tcgacaccct cgaccaaatg aaacaggggc tggaagatgt ttctggtctg
    46861 ctggaactgg ctgtagaagc tgacgacgaa gaaaccttta acgaagccgt tgctgaactc
    46921 gacgccctgg aagaaaaact ggcgcagctt gagttccgcc gtatgttctc tggcgaatat
    46981 gacagcgccg actgctacct cgatattcag gcggggtctg gcggtacgga agcacaggac
    47041 tgggcgagca tgcttgagcg tatgtatctg cgctgggctg aatcgcgtgg tttcaaaact
    47101 gaaatcatcg aagagtcgga aggtgaagtg gcgggtatta aatccgtgac gatcaaaatc
    47161 tccggcgatt acgcttacgg ctggctgcgt acagaaactg gcgttcaccg cctggtgcgt
    47221 aagagcccgt ttgactccgg cggtcgtcgc cacacatcgt tcagctctgc gtttgtttac
    47281 ccggaagttg atgatgatat tgatatcgaa atcaacccgg cggatttgcg cattgacgtt
    47341 tatcgcgcgt ccggcgcggg cggtcagcac gttaaccgta ccgaatctgc ggtgcgtatt
    47401 acccacatcc caaccgggat cgtgacccaa tgccagaacg accgttccca gcacaagaac
    47461 aaagaccagg ccatgaagca gatgaaagcg aagctttatg aactggagat gcagaagaaa
    47521 aatgccgaga aacaggcgat ggaagataac aaatccgaca tcggctgggg cagccagatt
    47581 cgttcttatg tccttgatga ctcccgcatt aaagatctgc gtaccggggt agaaacccgc
    47641 aacacgcagg ccgtgctgga cggcagcctg gatcaattta tcgaagcaag tttgaaagca
    47701 gggttatgag gaaccaacat gtctgaacaa cacgcacagg gcgccgacgc ggtagtcgat
    47761 cttaacaatg aactgaaaac gcgtcgtgag aagctggcga acctgcgcga gcaggggatt
    47821 gccttcccga acgatttccg tcgcgatcat acctctgacc aattgcacgc agaattcgac
    47881 ggtaaagaga acgaagaact ggaagcgctg aacatcgaag tcgccgttgc tggccgcatg
    47941 atgacccgcc gtattatggg taaagcgtct ttcgttaccc tgcaggatgt gggtggccgc
    48001 attcagctgt acgttgcccg tgacgatctg ccggagggcg tttacaacga gcagttcaaa
    48061 aaatgggacc tcggtgacat cctcggcgcg aaaggtaaac tgttcaaaac caaaaccggc
    48121 gaactgtcta tccactgtac cgagctgcgt ctgctgacca aagcactgcg tccgctgccg
    48181 gataaattcc acggcttgca ggatcaggaa gcgcgctatc gtcagcgtta tctggatctc
    48241 atctctaacg atgaatcccg taacaccttt aaagtgcgct cgcagatcct ctctggtatt
    48301 cgccagttca tggtgaaccg cggctttatg gaagttgaaa cgccgatgat gcaggtgatc
    48361 cctggcggtg ccgctgcgcg tccgtttatc acccaccata acgcgctgga tctcgacatg
    48421 tacctgcgta tcgcgccgga actgtacctc aagcgtctgg tggtcggtgg cttcgagcgt
    48481 gtattcgaaa tcaaccgtaa cttccgtaac gaaggtatct ccgtacgtca taacccagag
    48541 ttcaccatga tggaactcta catggcttac gcagattaca aagatctgat cgagctgacc
    48601 gaatcgctgt tccgtactct ggcacaggat attctcggta agacggaagt gacctatggc
    48661 gacgtgacgc tggacttcgg taaaccgttc gaaaaactga ccatgcgtga agcgatcaag
    48721 aaatatcgcc cggaaaccga catggcggat ctggataact tcgactctgc gaaagcgatt
    48781 gctgaatcta tcggtatcca cgttgagaag agctggggtc tgggccgtat cgttaccgag
    48841 atcttcgaag aagtggcaga agcacatctg attcagccga ccttcattac tgaatatccg
    48901 gcagaagttt ctccgctggc gcgtcgtaac gacattaacc cggaaatcac agaccgcttt
    48961 gagttcttca tcggtggtcg tgaaatcggt aacggcttta gcgagctgaa tgacgcggaa
    49021 gatcaggcgc aacgcttcct ggatcaggtt gccgcgaaag acgcaggtga cgacgaagcg
    49081 atgttctacg atgaagatta cgttaccgca ctggaacatg gcttaccgcc gacagcaggt
    49141 ctggggattg gcatcgaccg tatggtaatg ctgttcacca acagccatac catccgcgac
    49201 gttattctgt tcccggcgat gcgtccggtg aaataagcat tacgttatgc tcacaacccc
    49261 ggcaaatgtc ggggtttttt atttaagctg ggtaaatgca gataatcgta ttctggcttc
    49321 gcgatttgtc gcctgcatca ccatccacgg actgaacgcc cacggcgtgg catcaatacc
    49381 gtgtaataca tcagctaaat cacaccattg ataatccatc acttcatcat cgttgatctg
    49441 taacgcactg gtggtgcgtg cggcaaatac cggacacact tcattttcca caatgccact
    49501 cggatcggtg gcgcggtagc gaaagtcagg atagatagat tcaggaggcg taatttccac
    49561 gccaagctca taacggcaac ggcggatcac cgcgtcttcg ctgctttctc ccagttgtgg
    49621 gtgcccacaa accgagttag tccacacgcc aggccacgct tttttgctca gtgcgcggcg
    49681 ggtaactaat aattgcccct tggcattaaa cagccaactg gagaacgcga gatgtaagag
    49741 ggtgtctgcc gtgtgtgcgg catacttttc cagcgtacca gtgggaactc cctgtgcatt
    49801 caataaaatg acgtgttccg tttgcataat ttctcacatg taattctgat cactcatcat
    49861 tatagataat tgaagagtga aatgattaac caagtgagcg tttatcgaca accgcccgtt
    49921 ttgagcggat gccgacaggt aaaatccatt taaaaaagta gcatcatata tttgaagcga
    49981 ctttcaatgg ccattgcaac acagtcagaa atgacaggaa tagagagagt aaaaagcaac
    50041 agcagactga atatagcgcg cattaaaaaa ttcatattat tctccatgct catttttctt
    50101 cagcttgcgc atcagaagcc acatacgtac tgtcctgact tttaaatctt tatcagagac
    50161 ggattccttc accaggtcag catgatgctg gtagccatta aagaagacat tcagcacaac
    50221 ggcgctaagg gttgcgagca taatgccgct atgcagcaac ggttgcagta cggccggtaa
    50281 cttagaaaag aaatcgtgag agagcgtcgg agtcatgcca acgccgagac tgatcgccac
    50341 aatatagagg ttgtaacggt tggtggtgta gttacagcgc gacagaattc gaatccctgt
    50401 cgccagtacc atgccgaaca tcaccagccc agcaccgccc agcacaaatt gcggaatgga
    50461 ggcgaccagc accgccattt ttggcaccat gccaaacagg attaatataa ttcccgaaga
    50521 aatacacacc cagcggctat gaacgcgcgt cacgctaacc aggccaacgt tttgtgaaaa
    50581 tgacgtgtgg gggaagctgt taaacgttcc gcctatcatt gtccctacgc catcgacacg
    50641 cagcccgcga ataatatcgt gtgaagagag tttacgaccg actatttcac ccagtgccag
    50701 gaacatcccc atcgactcga taaacacgat gattaacacg gcagtcatgg tcagaatgga
    50761 aacgggatcg aaaatcggca taccaaatga catcggcgtg acaatcgcaa accatgaagc
    50821 atcatgtagc ccggataaat tgacttcatt catcatccac gaaagtaaaa agccaaatac
    50881 aatccccagt aatacggcga cgttggacat aaatcctttc gcatagcgag taatgagcaa
    50941 gataaaaatt aagacagcaa aggagatacc taaataaacg ggattaccat attgcggatt
    51001 ccctttacct ccggcggccc agtcaatacc cacctgaatg atgctaagcc caatagaagt
    51061 aataaccaca ccggtaacca gtggcgggaa taaaggcatc aagcgaccga taagtggcgc
    51121 taataatgtg gtgataaaac ccgcggcgat agtggcacca aatatcccca gcaggccgat
    51181 atccgggttc atcccaatgg ctatcattgg tgttacagca gcaaaggtca ccgacataat
    51241 caccggcagg cggatcccca taaagcggcc gataccgata cattgcaata atgtgacgat
    51301 cccgcagcaa aagagatccg agctaatgag catcgcaata gcatcttttg agaggcccag
    51361 tcggtcacca atcataagag gaacagcgac tgcacctgcg tacatgacaa ggacgtgttg
    51421 cagaccgagt atgatcagct ttcctggtga taatatgcgg tcaacctcat cagttgggcg
    51481 gtcttgccca gaagatgagg gaagttggga atctatggcg ctcatcagtg tttctccttt
    51541 cgcccgccag agtaaaaagc aaccgtttac gttaatgaaa ctgtctaaac acatgctcgt
    51601 ggagtgccag tttttgcgag gaaggggagt tagagacagg aattgcggag gcaatcacat
    51661 aatcgtgctt aaaccacgaa aatgacaaga gaacctgaca atggaattat ctgtaatttt
    51721 ctcatattga tatttcatta atcattctgg catgtcgcac tctcgcattt aatcgttttt
    51781 atctggatag cgctcttttg gtcggcaatg ttgaaataat gaaagcggca tagattgagg
    51841 ggcaataatt tgaactcagt cgcaaaatta taattaaaac ggtcatgcta cctaatatat
    51901 gcccgtttat tgcatcgcga aatttgagaa gacataactc attaaatgaa agggatgcaa
    51961 atgaataagt ttatcgctgc tgaagctgcg gaatgtatag gctgtcatgc ttgtgaaatt
    52021 gcctgtgcgg tggcacacaa tcaagaaaac tggccgctga gtcacagtga ctttcgaccg
    52081 cgtatccacg ttgtagggaa aggccaggct gcgaatccgg tggcctgcca tcactgcaac
    52141 aatgcccctt gcgttacggc ttgtccggtt aatgctctga ctttccagtc cgatagcgta
    52201 caactggacg agcaaaaatg tattggctgt aaaagatgcg caatcgcttg cccctttggc
    52261 gtcgttgaga tggtcgatac gattgcgcag aaatgcgacc tttgtaacca gcgcagttct
    52321 ggcacgcaag cctgtattga agtctgccca acacaggcgt tacgactgat ggacgataaa
    52381 gggttacagc agataaaggt ggcccgccag cgcaaaacgg cagcaggaaa agcgtcatca
    52441 gtcgctcagc catctcgcag tgcagcgttg ctccccgtta actcgcgtaa aggcgcagat
    52501 aaaatttcag caagtgaacg gaaaaaccac tttggcgaaa tctattgcgg gctggatcca
    52561 ctgcaagcga cttatgagag tgaccgctgt gtttattgtg ccgaaaaagc taactgcaac
    52621 tggcattgcc cgctgcataa cgctattccg gattacatcc gtctggtaca ggagggaaag
    52681 attattgaag cggcagaact ttgccaccag accagttcct taccagaaat ctgcggcagg
    52741 gtatgtccac aggaccgtct ttgtgaaggc gcatgtactt tgaaagatca ctctggcgca
    52801 gtcactatcg gtaatctgga acgctacatc accgataccg cgctggcgat gggctggcgt
    52861 cccgatgtca gcaaagttgt tccccgtagc gaaaaagtgg cggtgattgg cgctgggcca
    52921 gcagggttag ggtgtgctga tattctggcg cgcgcaggag ttcaggttga tgtctttgat
    52981 cgccatccag aaattggcgg tatgctgact tttggcattc ctcctttcaa actcgataaa
    53041 acggtattaa gccagcggcg agagatattc accgcaatgg gaatcgactt tcatctcaac
    53101 tgtgaaattg gccgcgatat tacctttagc aatttaactt ctgaatatga tgcagttttc
    53161 atcggcgtgg ggacttacgg gatgatgcga gcagatctgc cgcatgaaga tgcgcccggt
    53221 gtcattcagg ctctaccgtt cctgactgcc catacccgcc agctcatggg attgccggag
    53281 tctgaagagt atccgctgac ggacgtggaa ggtaagcgag tcgtggtatt gggcggtggc
    53341 gatacgacaa tggattgttt gcggacttcc atccgcctca atgccgccag cgtgacctgc
    53401 gcgtatcgtc gtgatgaagt cagtatgccg ggctcgcgca aagaggtggt taatgcgcgc
    53461 gaggaaggtg tcgagtttca gttcaatgtt caaccgcaat atatcgcttg tgacgaagat
    53521 gggcgtttaa ctgcggtggg cctgattcgt accgccatgg gtgagccggg gccggatggt
    53581 cgccgtcgtc ctcgtccggt agcgggttca gagtttgaat tgcccgccga tgttctgatt
    53641 atggcctttg gtttccaggc gcatgccatg ccgtggttgc agggcagcgg aattaaactc
    53701 gataaatggg gcctgattca aaccggtgac gtcgggtatt tacctaccca gacgcatctg
    53761 aaaaaagtct ttgctggtgg tgatgcagtt catggcgcgg atctggttgt cactgcaatg
    53821 gccgcaggaa ggcaggcggc gcgcgatatg ttaactctgt ttgatacgaa ggcatcgtga
    53881 tgaaatcgtt aattatcgtt aatccggctg actgtattgg ctgccgtact tgtgaagtag
    53941 cctgtgtggt cgctcaccct tcagaacagg agttgaatgc cgatgtcttt ttaccccggc
    54001 tgaaggtaca gcgactggac agcatcagtg cgccggtgat gtgccatcag tgtgaaaacg
    54061 ccccttgtgt tggcgcttgc cccgtggggg cgctgacgat gggcgagcag gtggtgcaga
    54121 ccaattctgc ccgttgtatt ggctgtcaga gttgcgtcag tgcgtgcccg tttggaatga
    54181 tcaccattca gtcgttgccg ggggataccc gacaacaaat tgtgaaatgc gatctctgtg
    54241 aacagcggga agaggggcca gcctgtgttg aatcttgccc gacacaggcg ctgcagttgc
    54301 ttactgaaag agaactcagg cgagtccgcc agcagcgtat tgttgccagc ggtgagaatc
    54361 ctctgtgaaa gaaatctccg cgctgaaagg cgcggagaat ctattagatt gcccaaccac
    54421 ccgcgtaaaa tgcgaccagt gcggcagtaa tgatcaccgt accaatattc agcttttgcc
    54481 attcgcgtgc aaagacgcgg cctacgacca gtgtcacaaa gcccagcata ataccggtaa
    54541 cgatattaca agtcagaacg atgaacacgg cacacaccag gccagccata gcgtcaataa
    54601 aatcattgaa atccagcttc gagacgttac ttaacatcag caaacccacg tacatcagag
    54661 cgggtgcagt ggcgtaacca gggatcaaaa atgacagcgg tgataagaac agaatcagca
    54721 ggaataacgc ccccactacg gttgcggtca accctgtttt acccccggcg gcggttcctg
    54781 ccgctgattc gatataaacc gctgcgggcg ctgcgcccac cagaccggag aatattgaac
    54841 ttactgagtc actggtcagg gctttgccgc cgttgatgat ctggttgtct ttatccaaca
    54901 aattcgcctg accggcgacg gcacggatgg tgccagtggc gtcgaacact gcggtcatca
    54961 ccaatgccag tacactcgga agtacagttg gctggagtgc gcccataata tcgaggctga
    55021 aaatcagaga cttaccatct tcgccagtca ggcttggcat cgccaccaga ccgtggtatt
    55081 tcaccgctgg gtcaaagatt aagccgatga tcgaaattgc aataatcacc aacaagatcc
    55141 cgccgggtac gcgacacttc tccagaccga agatgaccgc cagccccagt aagctcatca
    55201 tcaccgggaa ggaggtaaac gcaccgagcg ccactggcaa gccttcaatc gggtttttga
    55261 taaccattcc cacaccgtta gcagcaatca gcagcagaaa caggccgata ccgatacctg
    55321 taccgtgagc gatccccatc ggtaaattac gtaagatcca ggtacgcaca ccggttacgg
    55381 aaatagcggt gaagatgacg cccatcagaa agaccgcgcc cagtgcgaca ggaacgctaa
    55441 tttgttgccc gagtaccaga ctgaatgcgg taaacgccgt caaggaaatc gcgcaaccaa
    55501 tcgccattgg caaattggcc cataatccca tcagcaacga gccgaagccc gcgaccagac
    55561 aggtggcaac aaacacagct gcgggaggaa aacctgcttt gcccagcatt cccggaacga
    55621 cgataacgga ataaaccatg gccagaaagg tcgttaagcc agccagtact tcctgacgaa
    55681 cggtactgcc acgagcggta attttaaaat aattatccag cgcgccctgt ggctttggtg
    55741 cgtccggtgt ttgtaggatg tctccagaca taatgatgtc ctcacgagga tgttaaagtt
    55801 ttattattag ttgcgttcgt acaccagacg accatcaacg taggtgcggt agatcgaacg
    55861 gtcatcgccc aacgtcatca tcacgaacaa tttgtcgact aaagaaacag agttgtcata
    55921 gcgcagctgc tgtagcggag tggcggtggg ttccatcacc acgaaatcag cctctttgcc
    55981 aggtaaaaag ttgccaatca aatcgtcaag gcccagagat ttcgctccgc cgagcgtggc
    56041 caggtaaaac gcttcatatg ccgagaggcg atagccttgt aattgcaaca ctttgtaggc
    56101 ttcgttcagc gtttgcagca tgttgaaagt ggttccggca ccgatatccg ttcccatgcc
    56161 cactttaact ttcttctgcc atgctttttt caagttgaat aagccgctgc cgaggtaaag
    56221 gttggaggtc ggacagaaag caatgctgga tttggtttcg ctgagacgat cccactcttt
    56281 ttcttcgaga tggacgcagt gagcaaagac acagttttta ccggtcaggc cgtactgatg
    56341 gtaaacatcc agataaccat catggtcagg ataaagcgat ttcacccagg caatttcatc
    56401 tttgttttca cagagatggg tatgtaccca cgtatccgga tattcttctt tcaggcgttg
    56461 cgccatcgcc atctgttcag gagatgaggt cggggcgaag cgtggcgtaa tcgcatatag
    56521 cagacgacca tttttgtgcc agcgttcaat cagttctttg ctttggtgat agctgctttc
    56581 ggcagtgtcg agcagataat ccggtgcgtt acggtccatc atcaccttac cggcaatcat
    56641 acgcatattg atatgactgg cggcttcaaa cagcgcatca acggattgcg gatgaacagt
    56701 gccaaacacc agcgccgtgg tggttccgtt acgtaaaagc tgcttgatga agaacgccga
    56761 catttcgcgg gcgtactcta aatcctcata acgccgttca gtagggaagg tgtgtttatt
    56821 caaccactcc agcaattgct caccataggc ccccaccatt tcactttgcg gataatggat
    56881 atgtgtatcg acaaagcccg gtactatcag tttgccgcga tagtcgcgca cgcgaatggt
    56941 gtcaggaatt tgatgctttc cgttttccca ttcgccaaac cattccactt ttccctgttt
    57001 aatgagtaat aaaccatcct caataaaccg cagcgcagag gcaatctctt ccgggttatc
    57061 gaccgtacgg gtgacatcaa taaaactgcc tcgtaccgct tttaacgtgt gttctcctga
    57121 catcattgac tcctttactg ttaatccatc tcttccgctg aggtaatacc aggcagaacg
    57181 ttttcctgtc ggtagccacc agggaggata atatttaata aaatcgcagt taacccgcca
    57241 gcacaaatag ggttttcaac taatacataa atagaggctg gcaatatttt aaaaatttcg
    57301 ggatcgtagg agacgccaag ccctaaacct aaagaagtgg cgacaataag tgtttcacgg
    57361 cgctttaaac cgttggtgat gatgatgcga atccctgcga tggcaatcat ggaaaacatc
    57421 aacgtcattg cgcctcccag aactgccgag ggaatggtcg tgaagaagcc gccaatcatc
    57481 ggaaataagc cgaggataac cagcattacc gcgatggttc gcccgacata acgtgaagcg
    57541 acgccagtca tctgaataac cccattattt tgcgcaaacg tggttaatgg taatgaaccg
    57601 acagcggagg cgataacaga aaccagacca tctgccagca cgccgccttt cagtcgggac
    57661 tgatactctt ccccctgaat ggggcggcgg gaaaccattg ccgtggcggt gatatcgccg
    57721 actgcttcca gcacgctaag cagataaatc gtgccaacca ccaggaactg atggaagcta
    57781 aaactaaagc cgtatttgaa tggatgtggg atggtgatta acggcaaatt gcgcatactg
    57841 ctgaaatcca ccatgcccag gcataacgat gcgatatagc cgacacatag cccaatagcg
    57901 atccctccca tgcgtagcaa cggactgcga cagcagttaa agccgatcac cacaatcaaa
    57961 accaataaac caacgccgag atgttcgtaa ttgccgaagg taccgctgct tttggcagca
    58021 aatcctccac caaaatcaat aatgccgact ttaatcaggc ttaagccgat catcagtacc
    58081 acaataccgc tgacagtagg cgtaataacc cgacgtaaat agggcaggat aaaagaagag
    58141 ccgacaacca gaaatgcgcc aacgaaggag acgccgagaa gcgacgacat gattaactct
    58201 tcgtgaaaac cgtcgctttt catgctgctg cccagcgcaa tcatgaccgt aacaaatgaa
    58261 aaattgactg actgaattga gagtaggcca gaaccgacga tgccgtagcg gtttacttgt
    58321 aaccaggtac caataccaga ggcgatcatc gccattgaaa caagataggc agttgtttca
    58381 gcggaaagct gtaaggccgc acccacgatt aacgcggggg ttaccatcgg aacgaaaatt
    58441 gccaacagat gggtaatggc accaacgaga gcctgatgaa agggagggcg atcctccagt
    58501 tcaaatataa ggtcagaacc tgcatggttt atatcagaca tcccttcact ccttttaaag
    58561 taaatttgat catctgaatt agtaaaataa attcccacga atttggggcg caggacgcct
    58621 tgagaacggc ttatctcatt ttcgcagtca ctatttagtc atcgttcaaa cagagaaaca
    58681 ggccgccgaa gcggccattt attacttata ttttttccag cgcggtgagt attttctccg
    58741 gtgtgaaatg ccattcgcgt aaccagatgc cgcaggcatc gtgaattgcg gtagcaatcg
    58801 ccggagctgc gccatttaca ccgatttccg agatcgattt cgccccgaac gggccgactt
    58861 tatcgtcgct cggcaccagc acagcgcgga aatcacgcgg aatgtcacca attttcggtg
    58921 cgccgtaact gcgtaaatca cgcgttaacg ggtgaccttc ggcgtcataa atgatctctt
    58981 cgctcatact gtggccgata gctcgcaggg tagcaccgta gatttgtccc aatgctaatt
    59041 ccgggttgac cggtgtaccg cagtccagca aggcgtagaa tttatccagg cggatttcac
    59101 ccgtacgcgt gttgacggca acttcagcga agtttgcgcc atacgggaag gcgaaatcag
    59161 gcgtgatata actgccagtt cccaccagtg aaccaaagcc ggtgccggtt tcgcctttat
    59221 gagcaatatc cccgaagctg acttcgcctt tcttgccgcg cacgacgccc ggcgttgcca
    59281 gttgaacatc tgccactgcc tcacccaaca tttgcgcgcc gtggaacaga attttctccc
    59341 gcagattttc cgctgccaaa cgcgccgcgt tacctgagaa gcaagtaccg gacgaggcat
    59401 atgcgccttt atcaaacaac gcatgatcgg tatcaccgga gataacatgc acgtcctgcg
    59461 gtgggcaatg cagcacttct gctgccagtt tcgtcaccac agtatccaga ccagtaccaa
    59521 tatccgcacc gccagaatga acgataaagg tgccgtccga ttccagtttg atcatgcagt
    59581 tagcctgatc gatatccggg atacccgatt tctgcatgat aatggcgacg ccgcgaccga
    59641 tgtgccagtc accattttgc ggtttcggtg acgaccattg gatcatctcg cgaccctgac
    59701 gcaggatctc ttctagtgca cagctggcgg cggaaggaac ggaggtcggc gctttacctt
    59761 caccgattgc accgagaatt ttcagctctt gcccttcgtg tacccggtta cgttcgataa
    59821 tttccagttg gtcgatctgt aactgttcag ccagttccgc taatgccatg gtgatagcga
    59881 agttaccttt cggtgcgcca taaccctgat aagcaccatt tgggcaaatg ttgctgtagt
    59941 aggtggtgac ctgaaaatcg acgttatcgc acggatataa cggcagcgac agcgccggtc
    60001 cgttacatgg tacggtgagt gagtggttgc cgtaagggcc agtgttggcg cggaaatcca
    60061 tcttcactgc tgtcaggcga ccatctcttt tcgctcccag tttgacggtg actttcgcga
    60121 cgtgacgaga ggtgttagca ataaactctt cttcacgggt gtagcggaac agtaccggac
    60181 gcccggtcac gcaggttgcc caggcgcaca cttcttccag caggatgtcc tgtttggaac
    60241 caaaaccgcc gccgactcgc tctttaatga catgaacttt atgctgtttc atgcccacga
    60301 ggcgcgcgac ctggcggcgt aagtgccatg gtacctgggt ggaggcgtgg ataaccagac
    60361 gatcgccgtc catacgagta aagcagatat gtgtttcagt cgggcactgc tgcgcttgcg
    60421 ttgagttata ggttcgctca atgatcacat cggcatcggc aaagcctttg tccatatcgc
    60481 caatatgacc atgaatactg gcggcgatat ttttgcgggg gcgagaaccg atcgggaagt
    60541 taatgatcat atgctcgccg cgctgggctg catggctgtt atcgtcttcc agagtatctg
    60601 gcgcaccagc aacatacacc accggttcat cgtgcacgac aggcgcatct tccgccattg
    60661 cttcgtcgat cgacattacc ggcttaagca cttcatattc aacgtcgatg agcttcagtg
    60721 cttcgagcgc aatttcttca ctttctgcga ccaccgcagc aacacgatcg ccgacgtgac
    60781 gcattttctt gccgaacata cggcggtcaa gcggtgacgg ttccggtgcg ctctgacccc
    60841 ccggggtata gtagatatcc gggcaattca ggtgagtaat aacgtgaacg acgcccggta
    60901 aggcttcagc tttgctgaca tccagatgag taatcagtgc gtgagcgtgt gggctacgta
    60961 acattttaat gacgcaggcg tcagccgtta cgcggtcttc aacatagcag ggtttcgcct
    61021 gcaccatttt cgcggcatca gttttaggat aatgcttgcc aatgacttca aggtcgtcac
    61081 ggaaagtcgg agcgatatca atggtggcct gcggattatt tttacgtgca accgccagtt
    61141 caatgacctg atagtattgt tgccagcctg catcgcggct gaacaaacca gaaagcgcgt
    61201 cgtcgatctc ttcgcgggta ggtgcggcga tgcgatcgag aagatccgtg ataatcagag
    61261 ctgcagctgg atcgttataa ccagactgca ccacgccaac atcaaccatt gcctgttgaa
    61321 ccagacttaa ctcgttccat ttgcccagag attctgcggt acgaatatct gccttctcta
    61381 actgtgcggc aataagcaag gacgcgttaa cgatattacc gttaaagatt attgcgtcag
    61441 aaccggcaaa cccgaagcca tcatcactgt tgcgtacaga gtgcattccc atgttaaaca
    61501 acagcttttg cacgttttcg cctggattaa cggttagctc ctgaggcgcg ccatttaaag
    61561 taaagtggat gatcatacgg cttcctcccc agcctgttgg cagtcggcat acaggtcggc
    61621 taccagaact cccgtgatat agcgtttata ggccacgctg ccccgcaaat cttcctgcgg
    61681 gaaaatggcg ttggcgacag cctgttcaag tgcattgcct tccagatttt gtttttcgac
    61741 atcgtgcaga cgcagtgctt tactggcgac gccatccagc gcaattcgca taccgtcgtg
    61801 gtctgtcatt gcaacggctg ccgtcacgac ggttaaacct gcctgagaac ggctaatttt
    61861 gcgggtcgca caggtgcgat acggatcttt aatgataatt tcggttagca ggcgatcgca
    61921 tgggctggcc aggtagtcct cgattgacag cgtttcgccg ttgccaaaaa ccagttcagc
    61981 atccagtgcc agcaggacag gaagcagcac cgactcttcc tgccgggcgg cgatttcacc
    62041 accaatggtc gactgattac gaacatggcg tgagtaaaca aaaccgaggg cttcacgcag
    62101 tgctgcagga ataaatcgcg catcacgcag tggctgcaag cgagacattg caccaatccg
    62161 cagtgcaccg ttatcccagt caacccagtc cagttccaga tcctgtaagg aaatggcaat
    62221 ctttttatcg gtacgggttg gtgtagcgtt gagtttgctg cccccggcga accagacggc
    62281 ttcatcctgg tagcggcgct tcagttccag cgcctgttcg acagagtcgg gcctgaaaaa
    62341 ttgttcaatc atcttctttc cttatttaac cctcttccct gcgggaagag ggcatctgtc
    62401 atttatgcca gcgtatccat ccgacgccac atactggcag cggcttttct ggcttgtgca
    62461 taaatggaat cgcaatcaaa gttaaactga cggtcttcat agaccatcac gccattgacc
    62521 atcacgctgt gaacgctgcc tgagcccata ccgaaagcga tatgcccggc gatattgtct
    62581 gccagcagcg gcgtcggcga gttgtaatcg caaatggtta aatcggcttt gtaaccggct
    62641 tccagaagac caaatttcgc ggcaaagttg cggctcatca gttcgttacc gttagtcagg
    62701 gctttggcaa aactgtcagg ccacagcgga ccacccgcat cgcgatgttt aaagaaggca
    62761 aatttcatct cttcaaacat gtccgaacca atgccgtccg ttcccaacgc cagattgcgg
    62821 atgtcgctaa ggtgatggtt gtagccgaca tggttgttca tgtttgaacg ggcgttatgc
    62881 accaggaacg catcgcgctg attgagtagg gcgatgtcat ctttcgacaa gtacagccca
    62941 tgagcgacca gcgttttgct gtcgatgaga tcgaattgcg ccagtcgtgc cagcaggtct
    63001 ttgccgtacc agtggtgact gtaggaaacg tcgtaaaggt cttccgcagc gtgaatatgc
    63061 aaaccacgac ctgtggcttt cacggcttca cgcagcatct ccagaccggc atctggcacg
    63121 gtaaacggcg cgtgagcacc gatatgtgct tccaccagat acggctcggt tgctgctttc
    63181 ttcgcctcat caatctgacg ggcaaagcgg atgttttctt ctacaccttc ctgcaactct
    63241 ttgatgccgt tgttacggtc agtagtttca aaacaggtca ttgcgcgcag gccaactttt
    63301 aaaaatgcgt cgcgcaatgt ggagagcgac ccgccgatat acgctggaga ggcatggtga
    63361 tcgataaccg atgtacatcc gctcttaatc gcttccaggg aacaaatcag cccgctgtaa
    63421 tagagcgact cttcatcaag ggcgcgatcg agccgccacc agagattttt cagcgttgag
    63481 atgaaatccg ggcagggggc gatgtttgcc ataattccgc gggaaagccc cgagtaaaaa
    63541 tggttgtgcg agcagacaat tcccggcatc acaatccgac catgcatctc tttgtagctg
    63601 gcatcggggt agcgttgcgt cagggcatcg ccgatagcga caatcacatc attttcgata
    63661 gcgatatcaa cggcttcctg cactttcgcc gggtgtaact gcactgcggt gacattcttc
    63721 agaatcaaca tgattacacc tccacgcggc ccagcagata atgatgatgc tggtggacat
    63781 ggctgatgat gcggcacata tcgttcagct ccggtggtac gttgttgaac tgaccttcgc
    63841 tgtcgatgtt taacacccag ctttggttat tcagacgtac tcgtacccgg caatcttcca
    63901 caaggaagcc tgggttgctg ctgttatcaa agtcttgcga caggctgaag acggtgattt
    63961 tgtctttgta tggtttaccg ttccacggac agaactgagc gcagttgccg cattcgttac
    64021 agtaagcgtc gaggtgcagc gtctggaaac ggttctggaa gcctgggacc gcaatggaga
    64081 cgttggcgcg gttcgggcag acatccacgc acttgctgca aacgtagtta cattcaaggc
    64141 agcgagcagc ttcctgcgcg acaaacgcat cacgatcgtc actgttcacc agcgtgatag
    64201 agatatcgcc tttacgttga tagatttccg ccggattgac gttgttccag tatttatcgt
    64261 tctggtggga acggatattt tcccgactaa ggatggcatc ggtcgcccga cgcgcggttc
    64321 cgacagcagc gacaatggag gatggtccgc gctgtacgtc gccgatcata aagacgtcag
    64381 tcagacgagt ttcgccatta tggtcgacgt ctggccagcc gtttttgtcc agcggcacgc
    64441 ccatcgcatt cagggcttca gtatcctgct gttcaccaat ggcggtgatc aggctgtcta
    64501 catgcagtgt tactgtttcg ttggtttcaa ccggacgacg acgacctttc tcatccggtt
    64561 cgccaagcga cataacgcgc aaggttaagg tgccatcagc atcgaaacgt tccggattat
    64621 tcaggaaacg gaactccacg ccgtcgtgca acgcttcttc atactcttcg cgccatgcgg
    64681 gcatctcttg cagtgaacga cggtaaacga tcgttgcttt ttttacgcct ggaacgcgta
    64741 acgccgcacg agcgcagtcc attgcggtgt tacccgcccc gacaacgacc acatgtttgc
    64801 ccagcttgag cgctgtaccc ttgttgtatt cacgcaggaa ggggagtgat ttccagacat
    64861 tttggttgtc gcccgccagt ttcacaccgc tatttttatc agtgccggtg gcaatcagaa
    64921 catagtggaa gccctgattt tttaactgtt caacggtcaa atcgggtgag cagccatact
    64981 caaatttcac gccgtgagcg gcaacaaaat cgatatcgtg ctgaattaac tctgcaggaa
    65041 tacggaactg agggatgata tttttcacca cgccgcccgc attggcttcg cgttcaaaca
    65101 gcgtaaccgg atggcccgct ctggcaagga agtaacctgc agccagaccc gccggacctg
    65161 caccaatcac ggcaaccgga tggcgtgaac cagaaccggc tggtttgtgc cagcgttgct
    65221 tatattcatc ccaacctttt tccagcgcga cttttttcag ttcgcggata ttcagtgcac
    65281 tgtcgtaatc caggcgggta cagttgtatt ggcactggtg atcgcaaata tgaccggtaa
    65341 tggcgggcag ggcgttgcgt tggtagataa gttccagcgc gtcggcatag cggtgttcgc
    65401 caagcagacg gatgtattcc ggaatatctt gcttgatcgc gcaggcagta acacaggggg
    65461 caacgtagca gtcggtcagc ggcaggtctt ctgccacttc aatacgctct tctggcttcc
    65521 agtgtttctg ggtgtattcc atggttagcg cgtctgctgc cagtctgttc agtcgttcga
    65581 cgtcaacatg gtcaagtccc caggcgtcgg agccttccag ctcgcgcatg caggcactta
    65641 aacgcagata gccaccaggt ttcagcaggt cggttgccat agtaataggg cgaatacctg
    65701 tatcaaaaat atcgcggata gtcagctgac tggcaccacc ggaataagaa atgggcagtt
    65761 tgccgtcaaa ggcgcgagag agaactgctg caacattgat ggagagcggg aacagcgcac
    65821 ggcctgacat atacatctct tcaccaggca gtgcgccttt attgttgatg gtgccgagag
    65881 tgttagtcag ttttacgcca aagccgagtg atttttcttt tgccagtgcc atcaggcgtt
    65941 ccagcatttc cagtgcttgc gtcagcttga ggtcgtgatc aaatgactct tcttttaagc
    66001 ctatgtaacc gaaaccgcag acatcgagga tctcacgaac acgcgcgtac cccagtaagg
    66061 tcgggttaag tttcacaaag gtgttgagcc ctttttcttc cagcatgtag cggcaaatgg
    66121 cttcgatttc atgcggagga cagccgtgca tggtggagag ggtgacgcca tgcaccatac
    66181 tggtggggat gcgagcgggt aaggcttgca agctttcgcg tttttcctgc aatccgtggc
    66241 gggctaaaaa tgcgtcatcc tggagtaatt tattcagcgt atcgcgatat tgcgcgaatt
    66301 tcggatggtc agatgcgtcc atcatattgt cgatgaactg ttgcattggc ggttgcttaa
    66361 taccttcgag gttgtaaccg acgctcatat taaagatgaa cgatttaccg gaatcagaag
    66421 gttggaacat cgcttcgaga aggtgcaggg caaaccaggc tttgaggtat tcatcccagg
    66481 ctttaagcag ggtaaattcg gtagaccatt cggtgttaaa gcactcgtct tcggcatcaa
    66541 tacagggctt ttccagctcc aggcggtcaa gaatttggac ggtttttagt tcgatgaaac
    66601 gtccgccagt cagccaggaa gtgacaatat tttgcgcgag ctgcgtgtgc gggccagcgg
    66661 cagggccgac gggagtggca caggtttcac cgaagacgct aacagtttta ccttttacag
    66721 gtgagtaaaa ctgttgctcg ggaataccaa agattgagcg ttgttgttgg tattcatcaa
    66781 atatgcgcgt caaaagttcc tcaaacggaa tgggacgcat aatatccccc ataaccctct
    66841 ccttagctta tacagatatc attgagtgac cggtgttgag aaatccggtg gtttaggggg
    66901 atagagcaac aatcgcacca ctcttgccag gtttggcggg aaatataaaa aaatgtgagg
    66961 cggctcgaaa cttaagggct ttttgtgaat gaactcacag gtgctaaatg agaattaaaa
    67021 tcactcctga aaattattat tgccagccca tatatcactt tttctctttt ctcaaaatca
    67081 gtgattcctt atcattatga taaagagtgc tggagggatt aaatatttga acgcaatcgt
    67141 ttaactacgt tttttaaatt ttagtatacc ttttttgcgc tgtaataaag tcgtccggag
    67201 tatcaatatc taaaattatt tctgcatttt ctacttcgac ggaataatga gctcccataa
    67261 ggagtgcctg acgcatattg gtgatattgg ggcgcttgat tgcctgcatc aggcatggtt
    67321 ttgacactaa aatcggatgg ccggggatgc cattatggag tggcagtatt gcgccatcat
    67381 ttcgtaacga ccagattttt ctgaaaatat cgatggtgag ggttggcatg tcaccgtggg
    67441 tgagaaaaca atgttctgtt tgcactgcgg gtactgcggc ctttactgat gttagtaaac
    67501 cctgcgcata atctgggttg tggataatag tgatattgct ctggttcgca taacgttcgt
    67561 gcagttcatt accacgatag ccggtgacta aaataattcg gctacaaaac tgcaacgcat
    67621 ttttgatact tgtatcaaga attgtcccct gttgccaggg taacatcatt ttccattgtc
    67681 ccattcttga tgataatccg gcagcggtaa ttatacagtc gatggctgac atagtggttc
    67741 cattatttat aggggctaat aacgcctgat aattgcgtta cttcctgatg gattaagttg
    67801 gggataattt atcccagaga ggtcataaag actcgtgaaa agtatagttg acccatcggc
    67861 attattcatt gatttaggtg cgcagaaacg tcccactgtg atttctgttg ttggtgctgg
    67921 ggggaaaacc agcttgcttt tttggctggc agaactgctc caggctagcg gcaggcgtgt
    67981 gttaatcacg acgacaacac atatgtttat gccaacgtct cactggcccg tggttttctg
    68041 tcgtgatccc gccatgcttc ctcatgcgtc tcttacatcc cccatttcat tttgttttca
    68101 cagctggaaa gcgaaccagg gaaaagtgca gggatttacg ccagaagcga ttgatgcact
    68161 ggtgcaacga ccagagtgtg acgtaattct cattgaagca gatggctcgc gtgcaatgcc
    68221 gttaaaagcg cctgatgagc acgaaccttg catacccaaa agcagttgct gcgtgattgc
    68281 tgtgatggga ggacatattt tgggcgcgaa agtgagcaca gaaaatgtcc atcgctggtc
    68341 gcagtttgct gatattaccg ggttaacacc tgatgcaacc ttgcaactga gcgatctcgt
    68401 tgcgctggtt cgccaccctc agggggcgtt taaaaacgta ccgcaaggtt gtcggcgagt
    68461 ctggttcatt aaccgttttt ctcaatgtga gaatgcgatt gcgcaaagcg agctccttca
    68521 accgctgcaa caacacgacg tagaggcaat ctggctgggc gatatacaag aacatcctgc
    68581 aatcgcgcgc agatttgtga attagcggcc cgggtattcc gggttactga aaggatatcc
    68641 attgaggttg gtatgaatat tttcacagag gctgcaaaac tcgaagagca aaattgtccg
    68701 tttgcgatgg cgcaaattgt tgatagccga ggctcgactc cccgccattc tgcacaaatg
    68761 ttagtgcgcg ccgatggttc tatcgtcggt acaattggtg gcggaatggt tgagcggaag
    68821 gtgattgaag agtcacttca ggcattgcag gagcgtaagc cgcgattatt ccatggacgt
    68881 atggctcgta acggtgcgga tgctgtcggg tcagattgtg gaggtgcaat gtcagtattt
    68941 atcagcgtcc atggtatgcg tccacgtctg gtgttgattg gcgcggggca tgtcaaccgg
    69001 gcgatagccc agagtgcggc gctattagga tttgatattg ccgttgccga tatttatcgc
    69061 gaaagcctca atcctgaact attcccacca tcaaccacgc ttctccatgc tgagtcgttt
    69121 ggtgcggcag tggaagcact ggatattcgc cctgataatt ttgtcctgat tgccacgaat
    69181 aatcaggatc gtgaagccct cgacaaactc attgaacagc ccattgcatg gttggggttg
    69241 ctggcaagtc gtcgcaaggt tcagcttttc ctgcgtcaat tgcgtgagaa aggcgtggct
    69301 gaagaacata ttgcccgttt acatgcgccc gttggttaca acataggtgc ggaaacgccg
    69361 caggagatcg ccatcagcgt gctggcagaa atattacagg tgaaaaataa cgcgccgggt
    69421 gggctgatga tgaaaccttc tcatccttcc ggacaccagc tggtggtgat tcgcggtgcg
    69481 ggagatatcg ccagtggtgt ggcgctacgt ctgtatcatg cgggttttaa agtgatcatg
    69541 ctggaagtgg aaaaaccgac agtgattcgt tgtaccgtgg cgtttgccca ggccgtgttc
    69601 gatggcgaaa tgacggtcga aggcgtcact gctcgcctgg caaccagctc tgcggaagcg
    69661 atgaaactta ccgaacgcgg attcatccct gtgatggtag atcccgcctg ttcattgctt
    69721 gatgaactga aaccgctttg cgtggtggac gctattctgg cgaaacagaa tttggggacg
    69781 cgggcagata tggcaccagt aacaatcgcg cttgggccgg gctttattgc agggaaggat
    69841 tgtcatgcgg taattgaaac aaatcgcggg cactggctcg gtcaggtgat ttactctggt
    69901 tgtgcgcagg agaataccgg tgttcctggc aatattatgg ggcataccac ccgacgggtg
    69961 atccgtgctc ctgctgcagg cattatgcga tccaacgtga aattaggcga tctggtgaaa
    70021 gagggcgatg tgattgcctg gattggtgag catgaaatta aagcaccgtt gacggggatg
    70081 gtgcgtggct tgttgaacga cggcctggca gtggttggtg gttttaaaat tggtgatatc
    70141 gatcctcgtg gtgaaaccgc tgatttcacc agcgtttctg ataaagcccg ggcgattggc
    70201 ggcggcgtac ttgaggcgtt aatgatgttg atgcatcagg gcgtgaaagc gacaaaagaa
    70261 gtgctggaag tggcttaaat gaaaatggcc ggatggtatt acccacccgg ccttaaaatt
    70321 actgacaaat caccgttccg gttttgcctt caatcccttc tttcgctttg ctcaatacgg
    70381 taatcagtgc ttcacgacct gcgcgagagc gagcaaacga ggcggcagct tccactttag
    70441 gcagcataga acctttcgca aaatgacctt cttcgataaa gcgttcagca tcacttaatg
    70501 acaggcgatc gagccactgt tcattctctt taccgaagtt aatggccact ttttctaccg
    70561 cagtcagaat gatcagcata tcggcatcaa tcatttctgc taaacgggcg ctggcccagt
    70621 ctttatcgat aaccgcgctg gcaccacgca gatggttacc ttcacgaata acagggatac
    70681 caccaccgcc aacggtgatc accacctggc ctgcatctac cagagcttta accgtttctt
    70741 tttcaatgat atcaaccggt tttggcgagg caacaacacg gcgatagccg cgaccagcat
    70801 cttctttcag agtgtaaccc tgttttgtca gttgctctgc ttcttgctcg gtaaagaacg
    70861 aaccgatcgg cttggtcggg ttgaggaatg ccggatcgtt agcgtcgact tcgacctgag
    70921 taaccagcgt tgctacaggt ttattaatgc cccgagaaag cagttcttct cttaacgcgt
    70981 tttgtagatc gtaaccaata taaccctggc ttaaggcaac acagacagac atcggcagca
    71041 ttggcgagtg tgcttcagtt tttgcggcag cttcaaaggc ctggttaatc atgcccacct
    71101 gtggcccgtt accgtgagtg acgatgacct catgtccctg ggcaattaaa tcaacaattg
    71161 cctgagaagt aatttttacc gctttcattt gcccggccag atcatcgccc agcgcattcc
    71221 cgcccagggc gagaacaatt ttcttactca tttttcctca ctcatcattc ttgattgtgt
    71281 atagatccct ggcagtggtt agagcacggg agggacaaac ggtttgcgtc gcaggaaacg
    71341 acctcgcccg gctttgcctg taaaggtgcc gtcacagaaa atcgtttcac cacgggataa
    71401 ggttctgaca atcgcgccct gacaggtaaa accctcccat ggcgagtagt cggcgttgtc
    71461 gtggagatgg cgatgctgaa tttgttggct ctgacgtggg tcgataatca ccacgtcgcc
    71521 atcggaaccg ggcgctaata atcctttttg cggccacagg ccaaacagcc tggcgggcat
    71581 tgcgctggtt aattcaacaa agcgttccgg tgttatacgt cccgtcatca cgccactgga
    71641 aaacagtaac tgcatgcggt tttccacgcc gggtaagcca tttgggcagc gactgaaatc
    71701 gcctttagaa atttgcaggc gttgagccat tgagaacgtg cagtgatcgg tcgccaccac
    71761 gtcaatcgca ccatcgctga tgccacacca cagtttgtcc tgctcgcgta tgttacgcag
    71821 cggtgggcta agaatgaact tcatgccatc ttctgtatcg taactgcgtt cgtccaacag
    71881 gagatattgt gggcaggttt caacccagac tggctggtga ttcgcacggg caagacgcag
    71941 ataatccaga cctaagccgt tagacaggtg cacgatatag agcggggcgt taccggcaat
    72001 ttgtgccagg ttaatcatgc gggcgatggc ttccgcttcg cattccagag ggcgactcaa
    72061 ggcgtgatag cgcggcgcgg ttaaccctgc ggcgataaac tccgcccgct tgctggcgat
    72121 agccgcatca ttttccgggt gcacggtggt cagcgcgccg gattcatgca gacggcgtaa
    72181 tgcctgcaaa acctcgtcat cgttgagttt gtattgatag gttaaataga gtttaaaact
    72241 gctcagccct tcctcgacca tcatagggat ttcgtcgagg atggcgtgat taatgtgctg
    72301 gatcacacca tgaaagctgt agtcgatgac cgccttatgg gcggcatagc cacgataaac
    72361 ctccagttga tggcgtaacc gacacccgtt tgggccaaat cccatatggt caataatggt
    72421 tgttgtaccg ccacacgcag ctgcgcgggt accggtaaaa aaatcatcac aactgcgcgc
    72481 gatgccgaca tcaatattga aatgcgtatg gacatccacg ccgccaggga aaacgtaaca
    72541 gccagtggca tcaatttctt catacgggag ctgcggcgaa atattgttgc ccaactggcg
    72601 aacaatcccg ctttcaatca gcaaatcctg tttggcttgt ccatctgcgt taacgacagt
    72661 gccgtttttg atcaatacgc gcatagcaaa ctccaaaaac cctccggcga accggagggg
    72721 aataagtgaa gagtaattat tcggttgcca gccagcttaa cgggattgca gcgtacatcg
    72781 cagcacaggt caccaggtga gatttccagg ttttttcgtt aggtgcatgc gcttcaggtt
    72841 ctttacccgg gccaaagccg atcaccggaa tgccgtgacg gcccatgata gatacgccgt
    72901 tagttgagaa ggtccactta tcaacaaccg gcgctttgcc aaacagccct tcgtaggcat
    72961 tcaccagtgc tttaacggtg aagtgatctt cttccacttt ccaggtcggg aagtagcatt
    73021 cggttgggta aaccaggcca gtccaggacg gacggtcgta gttgtacata gaaacaaccg
    73081 cgttagcttt ctgtactgca ggcagggcgc ggatttcgtc cagcgcgcct tcccaggttt
    73141 cgccccaggt cagacgacgg tcgattgaaa ctgcgcagct gtctgctaca gcgcaacggc
    73201 ttggggatgt gaagaagatt tcagaaacgg tgagggtgcc tttgccgagg aattcgtcat
    73261 aacccagacg ttgggagagt tcttgtaatt cgccaagaat cggacccatt ttgaaaatgg
    73321 cgttatcacc gcgttctggt gcagaaccgt ggcagctaac accctgaaca tcaatacgaa
    73381 tttccatacg accgcgctga ccacggtata cctggcagtc ggttggttcg gtactgacca
    73441 caaattccgg gcgaatgccg gattgttcaa taatgtactg ccagcacaga ccgtcgcagt
    73501 cttcttcctg cacagtaccg gtaaccagca gggtatattc atcttccaga ccgaggtctt
    73561 taatgatttt accggcataa accatagatg ccatgccgcc ttcctggtcg gaagtaccgc
    73621 gaccaccgat tagctcatca gtttccatgc cttcgtacgg atcgaagtcc cagttcttga
    73681 tgttgccaat gccgacggta tcgatatgag cgtccattgc caccagacgc gggccgtggc
    73741 cgatataacc gagaacgttg cccatcgggt cgatttcaac tttatcgaag ccgacttttt
    73801 ccatctcttc tttaatacga tgtactacgc gtttttcgtc gcagctttca ctgggaatag
    73861 caaccatgtc gcgcaggaag cgagtcatgt ccgcctggta atcttttgct ttttcaagaa
    73921 tcagtttgaa tggaatattc ttagccatta tttttctcca gattttttac tccccgtttt
    73981 gcagggagta gttccaatag ggtgattaag gtgctacagc gtgtttgcct tcccagacaa
    74041 cttcgcggta gtgcttcacg tcggtgtcgc cttcagtact gataaccagc actacggcat
    74101 ctttgttcag cgccagtttt tccatcaggc tttgacgttg cgggtgataa tgaaccgctg
    74161 cgagaacgcc caaaccgaca gcgccggatt caccggagat gatgcgcggg tcgttgccgt
    74221 acggattacc cagcacgcgc atacctaatg cggcaacgct gtcctggcag gagatgaatt
    74281 gggtggcgca gttacgtagg atttcccagc ccagcgggtt aggttcgcca caggccaggc
    74341 ccgccatgat ggtggccata tcaccgccaa cgttgacgat gtcgcctttg acgccggagc
    74401 gataaataca gtcagctttg tcaggttcaa caataatgct gtgcagattt tgcgggctgt
    74461 agacgtcgac cagataaccc agcacaccac cggccattgc accgacaccg gcttgcagca
    74521 gaacgtgcgt cggggttacg cccatttcac gcatttgctc gacggcttca tctgccaggg
    74581 ttgcgtagcc ttgcatgatc caggttggga ttttggtgta accttcccat gccgtgtcct
    74641 gtaccacttc ccagccgtgc tgctgcgcgt gttgcatggt caggcgaacg gtatcgtcat
    74701 agttcatatc tgtgacgatg cactcggcac cgaggttcag aatggcgtca acgcgttcct
    74761 gagcagaacc tttcggcatg taaatcaccg cattctgtcc gagttgctgt gctgcccacg
    74821 ccacaccgcg cccgtggttg ccgtcggtgg tggtcgcgaa agtcattttt tcgccgatgg
    74881 catttttcag gtgctcaaat gacagcgttt cgatatcaag atgatatttt tcacacaata
    74941 attgagcgat ggcgtacgca ccgccaagca ttttgaaggc gttcagaccg aatcgttttg
    75001 attcgtcttt gacgagaatt ttcttcacac caaaaaggtt tgcgagatcg tccagcgcac
    75061 aaagcggtgt tgggcgataa ccagctattt tctggtggaa ctggcgcgcc agtttggcct
    75121 ggctttgcga aaagagcggt gatgtttcgc cgttgaaaaa tttgttatcg gcgatatcaa
    75181 tcttcaatga gaaaacggac atatcctatc cttttggcct ctggaaatag agggccgacc
    75241 agctgatcgg cccgaggggt tatttcacgc gttcttgcgc ttctttcagc agttgctcga
    75301 gcagtgcacc tggtttggcg tatttacggg acaggatcat cgcggcgatg atataaggct
    75361 tccagctggc ttctttgtag gtagcgatac ggtatttttc gaatacgcct tcagtcactt
    75421 caccttcttt gcaggatacg ccgctgatat cagctggcag gcagtgcatg tacagggctt
    75481 cgccatcacg ggtcagttcc atcatctctt cagtacaatg ccagtctttg tgttgcgcgt
    75541 tctgtgccag acactgtttt tccagtgctt ttaagccttc gtggtcgttc gcacgcagca
    75601 attcagtacg ctcttccatc actttgtaag gtgcccatga cttcggataa acgatgtctg
    75661 cgtctttgaa ggcttcttcc atgctggtga cctgacggaa gctaccaccg gaggctttag
    75721 cattgttttt cgccacttca accacatccg ggatcaggtc gtagccttcc ggatgggcca
    75781 gggtgacatc cataccgaag cgagtcatca gaccgatgat gccttgtggt acagagagcg
    75841 gtttgccata gcttggagag taggcccagg tcatggcgat ttttttacct ttcaggtttt
    75901 ccagtgaacc aaagtgttca cgtaaccacg cgaggtcagc cattgactga gtcgggtggt
    75961 caatatcgca ttgcaggttc actaaagccg gacgctgtgg cagtacaccc tgcttgtaac
    76021 cgtcatcaag tgcagcgcca acttcacgca tataggcgtt gcctgcgccc agatacatat
    76081 cgtcgcgaat accaatagcg tcggcgcaga aggagatcat attggcggtt tcacgcacgg
    76141 tttcgccgtg agcgatttgt gatttgcctt cgtcgagatc ttgttgtgca aggccgagca
    76201 ggttaagcgc ggaagcataa gagaagcggg tacgggtgga gttgtcgcgg aatacggaaa
    76261 tacctaatcc actattaaag actttggttg agatgttttc agcacgcagt gcttttaatg
    76321 ctgcggcaac gtccagtact tgtttcagtt catctggcgt ctgttcccac gttaacaaaa
    76381 aatctttctc gtgaaggtga gaggtcagcg aattgatatc cttaatcagc tcattaacag
    76441 ttttcatcat aaagtcccgg tagtggaatg acaggcaaag tggtttattt cactgtgact
    76501 tgccaaagtg attggtgttt tcacagctat acctgggact aacaataagt gtgccagaat
    76561 tatccgcgcg gctgcaaaag actaatttga cggataatgt gtggggttga tcacaaattg
    76621 atgccagctt ctggttgttt gtaaaatacg aaaacgtttg gcttttttta tcgcgttatt
    76681 tatttgctgc taagtatcag aaataagata aaggggcagg ctatctctct tcagtttgat
    76741 aaactcaccg ccgtaattct catattgata tagaaatgtg atatctcttc cataatttca
    76801 ttagaaaatg ataaaaatct tatgttttta tcaaagccaa tttcgcctga gttaatatca
    76861 tcatgatctg tgaaccatca acgtcttcaa tgatcgactt ttaagtaagt ctggaaggta
    76921 aagctatgga gcttgctact acgcagtcag tattgatgca aattcaaccg acaattcagc
    76981 gttttgccag aatgcttgcc agcgttttgc agcttgaggt tgagatcgtt gatgaaaact
    77041 tgtgtcgcgt cgccggaacg ggcgcgtatg ggaagtttct tggtcgccag ttgagcggca
    77101 actcacgcct gctccgccac gtcctggaaa cgaaaactga aaaagttgtg acacagtctc
    77161 gcttcgatcc cctttgcgaa ggttgcgata gtaaagaaaa ttgccgcgaa aaagcatttc
    77221 tgggtacgcc tgtcatttta caggatcgtt gtgttggggt gataagtttg attgccgtta
    77281 cccacgagca acaagagcat atcagtgata atttacgcga attttctgat tatgttcgcc
    77341 atatatccac catttttgtt tcgaaacttc tggaggatca ggggccagga gataacatca
    77401 gtaaaatatt cgcgaccatg atcgataata tggatcaggg cgtattagtt gttgatgatg
    77461 aaagtcgggt tcagtttgtt aatcagactg ccttaaaaac acttggtgtt gtacaaaata
    77521 atattattgg gaaacctatc cgtttcagac cattaacatt tgagagtaat tttactcatg
    77581 gacatatgca gcatattgtt tcgtgggacg ataaaagtga attaatcatt ggtcaattgc
    77641 ataacattca gggccgacaa ttatttttaa tggcatttca ccaatcgcat accagttttt
    77701 ctgtagcaaa tgcacctgat gaaccacata ttgaacaatt ggttggcgag tgccgtgtta
    77761 tgcggcaatt aaaacgactc attagccgta ttgcacccag cccatccagc gttatggtgg
    77821 ttggtgaaag cggcacgggt aaagaagttg tcgcccgtgc aatccataag ttgagcggaa
    77881 gacggaataa accctttatt gctatcaact gtgccgcgat tccggagcag cttctggaga
    77941 gcgaactgtt cggttatgtt aaaggcgcat ttactggcgc ttctgccaac ggtaaaacag
    78001 ggttgattca ggcggcgaat acgggcacgc tgtttctcga tgaaataggt gatatgccat
    78061 taatgttgca ggctaaatta ctgcgcgcta ttgaggcacg tgaaattctg ccgattggtg
    78121 ccagtagccc aatacaagtc gacattcgca tcatttctgc aactaaccag aatttggccc
    78181 agttcattgc cgaaggtaaa ttccgcgaag atctcttcta ccgacttaat gttatcccga
    78241 taactctgcc accgctgcgt gaacgtcagg aagatattga actactggtg cattactttt
    78301 tacatctgca tacccgtcgt ctgggatcgg tttatcctgg cattgctccc gatgtcgtcg
    78361 aaatattgcg taagcatcgt tggcccggaa acctgcgcga gttaagcaat ttgatggaat
    78421 atctggttaa cgttgttcct tcaggtgaag ttatcgacag cacgctattg ccgccaaatc
    78481 tgctgaataa tggcacaacg gagcaaagtg atgtaacaga ggtcactgag gcacacctgt
    78541 cactcgatga tgcgggcggc acggcgctgg aggagatgga aaagcaaatg atccgcgagg
    78601 cgctttcacg tcataacagc aagaagcaag ttgctgatga actgggcatc ggcattgcta
    78661 cgctctatcg caagattaag aaatatgagt tgttaaacac ataacctcag tgaatccggc
    78721 ctgaattcag gccggatatc cttttacttc gttttctcgc aatccagaac cgtatttaca
    78781 atcatctgat accccgtgca gcgacaaaga tttcccgcca gtccgcgacg aatttccgta
    78841 atggttaatg gcttctcgcg tggtttcgcc agcattgccg tggtagccat aatcaggcca
    78901 ggcgtacaaa acccgcactg caccgcgccg gatttcgcat aagcctgctg aacatgagaa
    78961 agttttccgc ctttcgcttc accttcaagc gtgcggatct cttttccttc agcccaggcg
    79021 gcaaggtata agcaactgtc tattgctgtg ccgtcgacca gcaccgtaca ggcaccacat
    79081 tcacccacgc agcacccttg tttgacactt agcagtcctt gttcgcggag taattccgag
    79141 agcggcgtac ctggtgcggc atgaagctga aaaggcatcc cgttaatggt gcattcgatg
    79201 gtaattgttt cgctgtgatt cattgcaatt ttccccccgc cgcggcgacg gcttcgctaa
    79261 tcactttttt ggtcatcgtc tggatgagat gcagacgaaa ctctttactg gcccgccatg
    79321 aagaacgcgg ggcgacatct tgcaggacag attcgctgat agcttccagc gtttgcaggt
    79381 ttaatggcgc attttgtgca gtctgttcgg catgttggca gcgaatcggc gttggcgcgg
    79441 caacaccaaa tgccaggcgt aattcgctga aattgccgtt atccagtcgg caatgtgcgg
    79501 cacagccaat cgttgaaata tccattgcgt cgcgcatggc atatttaaaa tgcgcgctgc
    79561 ccacgtgttc tttcggctgg ggcggaaaat gaaaggcgac aaggatttcg tcatgctcaa
    79621 gagacacttt gcctggccca gtgtgaaagc cattaatcgg gacgaaacga acaccgcgtg
    79681 gggagtggat ctccagcttt gcgtcataaa ttagcgttgg cgtggcagaa tctgcgctgg
    79741 tggcaccgtt gcaaatattt ccaccgtagg tagcgacgtt acggatctgc ggtccggcaa
    79801 tggatgaggc cgcagcacat aacgccggga gatgacgttg agttacagga tcttcaatga
    79861 gctgggtaaa tgtcgttgca gagccgatac gtagtgagcc atcttccgcc agcgtaattc
    79921 cccgcagctc cgccagatta tgaatatcaa caatatggcg ataacggtca ttgtgatggt
    79981 ggagctgaat cagtacgtca gtgccaccgg cgagcagttt ggcctgcggg ttgtcaacca
    80041 gcaggttgat ggcatcggca agggttgctg cgcgatggta agaagcaaaa tcaaacatga
    80101 tgttatcctc aaatcaatcc tgccagatgg aactcttcat ataaccgttt tggcgtcagc
    80161 ggcagtgtat tgattgcaac accggtagcc atcttcaccg cgttacgaat agctgcggca
    80221 acaggaatta ttggtggctc acccagtgac ttatgtccgt atgcggattg cggctcattg
    80281 atttcgacga acgcgctttc cagttgtggc agatccggca tggtcggcat tttgtaatcc
    80341 agcagattgg ggttacggac cacgccgctt ttcgcatcga tgatcatctc ttcaaatagc
    80401 gcccagccaa tgcccattcc cattccgccg tgtacctgac cttctgccag cagcggattt
    80461 agaatatgcc ccgaatcatg aacgttgagg atgcggttga tggtgacttt gcacagtgca
    80521 atatcgaccg tcagatcaac aaaggtacag ccgaacgccg gtgggttagt ggtggttttg
    80581 atggagcttt cagcagagag ctgcccgccg cgttcagggt ggtagaaagc gtccatcgcc
    80641 aaatctttta acgacattaa cggctcttcc ggtcgttcaa ccagcacgat atggcctttt
    80701 atcagggtca gattcatcgc tgactgatgt agcatgactg cggcgtgagc gatgattttc
    80761 tcttttaata acagtgccgc actgcgcagc gcaggcgcgg caacatagct ctggcgcgag
    80821 gcaaatgcgc cgggatcgaa cggcgtaacg tcggtatctt gtgttgaaat aacgcgaacg
    80881 tcgctgaccg gaacccccac ggtttctgcc accatttgcg agaagacggt gtcggcaccc
    80941 tgaccgattt ccgtcgcgcc gctttgcacg ttgatggttc catcctgatt catcagaagg
    81001 cgcgcgcctg ctatttctac gccgacaggc caggtattag aggtgtagct aaaacaggcg
    81061 acgccaacgc cgcggcgtaa attgccttgc tggttctggc attctgcacg gcgtttttcc
    81121 cattcaaaga ttttccggcc tttttcaaga cactccggca accctgcgct gtaaatacgt
    81181 ttgcccgtga gcggattagc atctccttcg cgggcggcgt tgcgtaaacg aatttcaaca
    81241 ggatcaatac ctaacgctgt cgcggcgtca tcaagcatag actcaacggc aaatacgact
    81301 tgtggcgcgc cataaccacg catcgcacca gccgagggga ggttggtata gcaggtcttt
    81361 gaactgtaag cgtaggcaca acgaggataa aggtaagcga ctttattccc ccccgcagaa
    81421 gcgatggagt gcccgtgaga tgcataagcg ccggtgttag acagaacatc cagactataa
    81481 cctttcaatg ttccgtcgcg gttcacgccc atttgcccgt caatagtaaa agcgtggcgg
    81541 gtacgggttg cgaggaaaca ctcttcacgg ctaagggaaa ctttcaccgg aatgccgcca
    81601 agcttgctgg tcaggaatgc cgccattggc tcttccagta catcctgttt attaccaaaa
    81661 ccgccaccga caaatggttt gatgactcgt acgcatgacc agggaatatc cagcgcctga
    81721 ccaaccacgc ggcgaacaat gtgcgggatc tgggtgctgg aaacgatggt aattcgcgag
    81781 tcatcctcca tccacgccag cgatgttacg ctttccatgt gacaatgttg aataacgggg
    81841 gtctgatagt gcccctgtac ctggtagtcg gcggcatcga ttgtttgttg gacattgccc
    81901 gtcgacatcg tgctttgttt cagtaaattg ccgccgttat ggattggtgc agcgtcctct
    81961 gccagcgccg cttctggcgt ggtgataacg ggtaattctt gccactcaat gctgaccaat
    82021 tgcgccgctt tttctgccgt gagttcatcg cgggccacga cgatggcaac ggcgtcacca
    82081 tgatgacgaa catggcgagt tagcagtgcg cgatcggcgg tatcgcgctt gttttcgtca
    82141 agtgtccatg catgtccagc tgtagcgaat gggatatcag gcacatcttc ccaggtaaaa
    82201 atcgccagta cgcccggtaa acttctggct tgttcatcat taatacttac ggcataacca
    82261 tgtgcgatag ggctacgtac atatttcgca taacacatgc ccgccataac ataatcgtca
    82321 gtatatcgtg cccgcccggt gaccttagca atggcatcga cgcgcatgca tgattcaccc
    82381 gtagcggttg cttcccgcgc ttccatagaa atcccctcga tttgaacatt tggttcttat
    82441 ggcaaagatt tagtctaagc aggcaagatt tacgccagaa acgacgataa gcgttagggg
    82501 aggaaggaag tgtgatgacg ttaaaaaaac acagatatga tacgaaacag gactctgaat
    82561 agggacagtg atacgtatca atatgagtgg cgataaaatc aatgtgataa atcaggatta
    82621 ttacgcatca ggatgggaaa tagggtaaaa agtcaggaac ttcgatacct ctacagcgaa
    82681 tattttgctg atgatacttc cctttgccaa atgaaattaa cctctttctc tattaccacg
    82741 tttttccaga agcaagagat tgggtatcaa gaggctggct gctatgatat ggcgtcttgt
    82801 tttttaagcg aggaaagatt ttgagtgcgg gacgcctgaa taaaaaatct ctgggtatcg
    82861 tgatgttgtt atcggttgga ctgcttttgg cgggctgttc gggtagcaaa tcatccgata
    82921 caggaacgta ttccggctcc gtttacaccg tgaaacgggg ggatacgcta tatcgtattt
    82981 cgcgcaccac gggaaccagc gtaaaagaac tggcgcgact gaacggcatt tccccccctt
    83041 acaccattga agttggtcag aaactaaaac tgggtggggc gaaaagtagc agtattacac
    83101 gtaaatcaac cgccaaatca acgaccaaaa ccgcatcggt tacaccgtca tcagcggtac
    83161 cgaaatcatc ctggccgcca gtagggcaac gttgttggtt atggccaacg acagggaaag
    83221 ttatcatgcc gtattcgaca gcagatggcg gcaataaagg gattgatatc tcagctccac
    83281 ggggtacacc tatttacgcc gcgggtgcag gaaaggtggt gtatgtgggc aaccagctgc
    83341 gtggctacgg taatctcatc atgattaaac acagtgaaga ttacattacg gcttacgccc
    83401 ataatgacac gatgctggta aataatgggc aaagcgtgaa ggctgggcaa aaaatcgcca
    83461 ctatggggag cacggatgcg gcatctgttc gcctgcattt ccagattcgt taccgtgcaa
    83521 cggcaattga tccgctacgt tacttgccgc cgcagggcag caagccaaaa tgctgatggc
    83581 gaattaatca gcagtcagca gcgcggcact tgctaaggag agcgtaaggt ttataatgcc
    83641 ttacgcatct cgaagcgggc gtagttcaat ggtagaacga gagcttccca agctctatac
    83701 gagggttcga ttcccttcgc ccgctccaat ttatcttcac ctcatcaatc tgccattttt
    83761 gctgtatctg attttctata ttatcttaat tcaatcgctt cagacttttc gcaatcgccg
    83821 taatatttcc ccattttgtt gtaaataaaa agcgtttacg caatgcaatc cttttgtaac
    83881 aacccaggta aaacacctta ttttcaagtt gttaaataag tggttatcta cgcttttaac
    83941 aaacgattga tggaatattt tatgaaaggg aaatctgcat taactcttct gctcgctggt
    84001 atattttcgt gcggcacgtg ccaggcgaca ggggcagaag tgacgagtga atctgtattt
    84061 aacattctta attccacggg ggcagcaaca gataaaagtt atttatcgtt aaatccagat
    84121 aagtacccta attatcgtct tctgattcac tcagccaaat taaaaaatga aataaagagc
    84181 cactatacca aagacgaaat tcaagggttg ttaacattaa cagaaaacac acgaaagtta
    84241 acgttgacgg agaaaccctg gggaacattt attcttgctt ctactttcga agatgacaaa
    84301 acagcggcag aaacccacta tgatgctgtc tggctgaggg atagtttatg gggctatatg
    84361 gcattagtat cagatcaggg aaatagtgta gccgcaaaaa aggttctgct caccctgtgg
    84421 gattatatgt ctacgcccga ccagattaag cggatgcagg acgtcattag caaccccaaa
    84481 cgactggatg gagttcccgg gcaaatgaat gctgtgcata tccgatttga cagcaactcc
    84541 cccgtgatgg cagatgtgca ggaagaaggc aagccgcagc tgtggaacca taaacaaaat
    84601 gatgcgttag gtctttatct tgatctcctt atacaagcaa tcgacactgg tacaattaac
    84661 gctgaagatt ggcaaaaagg tgaccgatta aaatcggttg cgctgctgat tgcttatctg
    84721 gataaagcca atttctatgt tatggaagat tccggagcat gggaagaaga tgcccgatta
    84781 aacacatctt ccgttgcact ggtgacctct ggactggaac gtttgtcgaa cttactctcg
    84841 aagaaagact ctgtttttgt ctctgattta ctacgcgaag ctaaagccaa tgaactggat
    84901 gaacctcttt caacaacgag gttgaaccac ctcatagata aaggttatga aagaataacg
    84961 ttacagcttg atttaggggg agaatcccca ggttatctgg aaaaagataa acattacaga
    85021 gaagccgatg cggctttact taatgttatt tatcctgcaa atcttgcaaa gataaacacc
    85081 agaagaaaag agcaggttct aaaaatagtt aaaaaactag caggtccgta tggtataaaa
    85141 agatacgaga aggataatta tcagtcggca aatttttggt tcaatgatat taaaaccgat
    85201 accgatcaaa attcacatgt gaaaagagaa aagagcttca ttccatcaac agaagcagaa
    85261 tggttttttg actcatggta tgcaaaatca gcagcaattg tttataaaga atcaagaaaa
    85321 gaagagtatt tgaatgactc agtgcaattt atgaatagat cattggccca aataacaggc
    85381 gagaatatga ttggtgcaaa tggcaggagt gtacctgaaa tggcacttcc cgaaagctat
    85441 aactatatcc acaagtcggg aactttgcat gaagcgccaa accctatcat tccgctaaat
    85501 tggtcgaaag cttcaatgac tctcatgttg aaagagatgt ccagccttat caatgatgag
    85561 ggaaataaat agttttcata atgaggcggt cctggattaa aaggaccgct caaagagaaa
    85621 tcgaaagggg aggtaaaagt gatcaataaa ttactgttag cttacctgat aggtctggtt
    85681 gtgacatcga cttttatatt tatcttctcg gaagaaaaag taacatatcg gttgtttgct
    85741 acaataatta caggtttaac ctggccgctt agtctcattc cttccattat aagtcttatg
    85801 attcgtaaat ccgactaaca agaaaattat atgatcgatt aagaaaaata gaatcgaaca
    85861 agagaataaa atatatgcgg ggagtaacaa aacactcgca gtgtgttaat gaagagctaa
    85921 caaatatgat ttgtagtgaa aatagttacc aaacaagtgt tataagaaat acgag
//
LOCUS       DAJOWB010000007        77581 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJOWB010000007 DAJOWB010000000
VERSION     DAJOWB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 77581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.007"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(74..745)
                     /gene="queE"
                     /locus_tag="OHO85_000551"
     CDS             complement(74..745)
                     /gene="queE"
                     /locus_tag="OHO85_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCQ2669978.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            884..1024
                     /locus_tag="OHO85_000552"
     CDS             884..1024
                     /locus_tag="OHO85_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2669979.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            1038..1910
                     /locus_tag="OHO85_000553"
     CDS             1038..1910
                     /locus_tag="OHO85_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCQ2669980.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIKVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSDSGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1970..3268)
                     /gene="eno"
                     /locus_tag="OHO85_000554"
     CDS             complement(1970..3268)
                     /gene="eno"
                     /locus_tag="OHO85_000554"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCQ2669981.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(3356..4993)
                     /gene="pyrG"
                     /locus_tag="OHO85_000555"
     CDS             complement(3356..4993)
                     /gene="pyrG"
                     /locus_tag="OHO85_000555"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCQ2669982.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(5221..6012)
                     /gene="mazG"
                     /locus_tag="OHO85_000556"
     CDS             complement(5221..6012)
                     /gene="mazG"
                     /locus_tag="OHO85_000556"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCQ2669983.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(6083..6418)
                     /gene="mazF"
                     /locus_tag="OHO85_000557"
     CDS             complement(6083..6418)
                     /gene="mazF"
                     /locus_tag="OHO85_000557"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCQ2669984.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPFTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(6418..6666)
                     /gene="mazE"
                     /locus_tag="OHO85_000558"
     CDS             complement(6418..6666)
                     /gene="mazE"
                     /locus_tag="OHO85_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCQ2669985.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(6744..8978)
                     /locus_tag="OHO85_000559"
     CDS             complement(6744..8978)
                     /locus_tag="OHO85_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCQ2669986.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(9026..10327)
                     /gene="rlmD"
                     /locus_tag="OHO85_000560"
     CDS             complement(9026..10327)
                     /gene="rlmD"
                     /locus_tag="OHO85_000560"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCQ2669987.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            10384..13140
                     /gene="barA"
                     /locus_tag="OHO85_000561"
     CDS             10384..13140
                     /gene="barA"
                     /locus_tag="OHO85_000561"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCQ2669988.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(13372..14712)
                     /gene="gudD"
                     /locus_tag="OHO85_000562"
     CDS             complement(13372..14712)
                     /gene="gudD"
                     /locus_tag="OHO85_000562"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCQ2669989.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAKRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(14733..16073)
                     /locus_tag="OHO85_000563"
     CDS             complement(14733..16073)
                     /locus_tag="OHO85_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCQ2669990.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(16075..17427)
                     /gene="gudP"
                     /locus_tag="OHO85_000564"
     CDS             complement(16075..17427)
                     /gene="gudP"
                     /locus_tag="OHO85_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCQ2669991.1"
                     /translation="MSSLSQAASSVEKCTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(17862..18311)
                     /locus_tag="OHO85_000565"
     CDS             complement(17862..18311)
                     /locus_tag="OHO85_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCQ2669992.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(18329..19111)
                     /gene="truC"
                     /locus_tag="OHO85_000566"
     CDS             complement(18329..19111)
                     /gene="truC"
                     /locus_tag="OHO85_000566"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCQ2669993.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(19111..19440)
                     /locus_tag="OHO85_000567"
     CDS             complement(19111..19440)
                     /locus_tag="OHO85_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCQ2669994.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(20062..20607)
                     /gene="syd"
                     /locus_tag="OHO85_000568"
     CDS             complement(20062..20607)
                     /gene="syd"
                     /locus_tag="OHO85_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCQ2669995.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            20675..21523
                     /gene="queF"
                     /locus_tag="OHO85_000569"
     CDS             20675..21523
                     /gene="queF"
                     /locus_tag="OHO85_000569"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCQ2669996.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            21635..22999
                     /gene="ppnN"
                     /locus_tag="OHO85_000570"
                     /gene_synonym="ygdH"
     CDS             21635..22999
                     /gene="ppnN"
                     /locus_tag="OHO85_000570"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCQ2669997.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            23556..24845
                     /gene="sdaC"
                     /locus_tag="OHO85_000571"
     CDS             23556..24845
                     /gene="sdaC"
                     /locus_tag="OHO85_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCQ2669998.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            24903..26270
                     /gene="sdaB"
                     /locus_tag="OHO85_000572"
     CDS             24903..26270
                     /gene="sdaB"
                     /locus_tag="OHO85_000572"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCQ2669999.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            26382..27137
                     /gene="xni"
                     /locus_tag="OHO85_000573"
     CDS             26382..27137
                     /gene="xni"
                     /locus_tag="OHO85_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCQ2670000.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(27192..28340)
                     /gene="fucO"
                     /locus_tag="OHO85_000574"
     CDS             complement(27192..28340)
                     /gene="fucO"
                     /locus_tag="OHO85_000574"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCQ2670001.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(28368..29015)
                     /gene="fucA"
                     /locus_tag="OHO85_000575"
     CDS             complement(28368..29015)
                     /gene="fucA"
                     /locus_tag="OHO85_000575"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCQ2670002.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            29562..30878
                     /gene="fucP"
                     /locus_tag="OHO85_000576"
     CDS             29562..30878
                     /gene="fucP"
                     /locus_tag="OHO85_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCQ2670003.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            30911..32686
                     /gene="fucI"
                     /locus_tag="OHO85_000577"
     CDS             30911..32686
                     /gene="fucI"
                     /locus_tag="OHO85_000577"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCQ2670004.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            32795..34213
                     /gene="fucK"
                     /locus_tag="OHO85_000578"
     CDS             32795..34213
                     /gene="fucK"
                     /locus_tag="OHO85_000578"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCQ2670005.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            34215..34637
                     /gene="fucU"
                     /locus_tag="OHO85_000579"
     CDS             34215..34637
                     /gene="fucU"
                     /locus_tag="OHO85_000579"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCQ2670006.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            34695..35426
                     /gene="fucR"
                     /locus_tag="OHO85_000580"
     CDS             34695..35426
                     /gene="fucR"
                     /locus_tag="OHO85_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCQ2670007.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(35470..36570)
                     /gene="rlmM"
                     /locus_tag="OHO85_000581"
     CDS             complement(35470..36570)
                     /gene="rlmM"
                     /locus_tag="OHO85_000581"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCQ2670008.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(36563..36958)
                     /locus_tag="OHO85_000582"
     CDS             complement(36563..36958)
                     /locus_tag="OHO85_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCQ2670009.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(36977..37894)
                     /gene="gcvA"
                     /locus_tag="OHO85_000583"
     CDS             complement(36977..37894)
                     /gene="gcvA"
                     /locus_tag="OHO85_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCQ2670010.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(38245..38472)
                     /locus_tag="OHO85_000584"
     CDS             complement(38245..38472)
                     /locus_tag="OHO85_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCQ2670011.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            38664..39869
                     /gene="csdA"
                     /locus_tag="OHO85_000585"
     CDS             38664..39869
                     /gene="csdA"
                     /locus_tag="OHO85_000585"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCQ2670012.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            39869..40312
                     /gene="csdE"
                     /locus_tag="OHO85_000586"
     CDS             39869..40312
                     /gene="csdE"
                     /locus_tag="OHO85_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCQ2670013.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(40363..41169)
                     /gene="tcdA"
                     /locus_tag="OHO85_000587"
     CDS             complement(40363..41169)
                     /gene="tcdA"
                     /locus_tag="OHO85_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCQ2670014.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAKVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(41408..42505)
                     /gene="mltA"
                     /locus_tag="OHO85_000588"
     CDS             complement(41408..42505)
                     /gene="mltA"
                     /locus_tag="OHO85_000588"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCQ2670015.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGADNVFSG"
     gene            42714..42790
                     /locus_tag="OHO85_000589"
     tRNA            42714..42790
                     /locus_tag="OHO85_000589"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42748..42750,aa:Met,seq:cat)
     gene            42824..42900
                     /locus_tag="OHO85_000590"
     tRNA            42824..42900
                     /locus_tag="OHO85_000590"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42858..42860,aa:Met,seq:cat)
     gene            42934..43010
                     /locus_tag="OHO85_000591"
     tRNA            42934..43010
                     /locus_tag="OHO85_000591"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42968..42970,aa:Met,seq:cat)
     gene            complement(43085..44338)
                     /gene="amiC"
                     /locus_tag="OHO85_000592"
     CDS             complement(43085..44338)
                     /gene="amiC"
                     /locus_tag="OHO85_000592"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCQ2670016.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            44570..45901
                     /gene="argA"
                     /locus_tag="OHO85_000593"
     CDS             44570..45901
                     /gene="argA"
                     /locus_tag="OHO85_000593"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCQ2670017.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERITAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(45963..47789)
                     /gene="recD"
                     /locus_tag="OHO85_000594"
     CDS             complement(45963..47789)
                     /gene="recD"
                     /locus_tag="OHO85_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCQ2670018.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(47789..51331)
                     /gene="recB"
                     /locus_tag="OHO85_000595"
     CDS             complement(47789..51331)
                     /gene="recB"
                     /locus_tag="OHO85_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCQ2670019.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIEQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDVVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(51324..54212)
                     /gene="ptrA"
                     /locus_tag="OHO85_000596"
     CDS             complement(51324..54212)
                     /gene="ptrA"
                     /locus_tag="OHO85_000596"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCQ2670020.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(54388..57756)
                     /gene="recC"
                     /locus_tag="OHO85_000597"
     CDS             complement(54388..57756)
                     /gene="recC"
                     /locus_tag="OHO85_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCQ2670021.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKSHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(57769..58092)
                     /locus_tag="OHO85_000598"
     CDS             complement(57769..58092)
                     /locus_tag="OHO85_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCQ2670022.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(58077..58484)
                     /locus_tag="OHO85_000599"
     CDS             complement(58077..58484)
                     /locus_tag="OHO85_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCQ2670023.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(58481..59044)
                     /locus_tag="OHO85_000600"
     CDS             complement(58481..59044)
                     /locus_tag="OHO85_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCQ2670024.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(59035..59505)
                     /locus_tag="OHO85_000601"
     CDS             complement(59035..59505)
                     /locus_tag="OHO85_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCQ2670025.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(59690..60484)
                     /gene="thyA"
                     /locus_tag="OHO85_000602"
     CDS             complement(59690..60484)
                     /gene="thyA"
                     /locus_tag="OHO85_000602"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCQ2670026.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(60491..61366)
                     /gene="lgt"
                     /locus_tag="OHO85_000603"
     CDS             complement(60491..61366)
                     /gene="lgt"
                     /locus_tag="OHO85_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCQ2670027.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(61517..63763)
                     /gene="ptsP"
                     /locus_tag="OHO85_000604"
     CDS             complement(61517..63763)
                     /gene="ptsP"
                     /locus_tag="OHO85_000604"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCQ2670028.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYADAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(63776..64306)
                     /gene="rppH"
                     /locus_tag="OHO85_000605"
     CDS             complement(63776..64306)
                     /gene="rppH"
                     /locus_tag="OHO85_000605"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCQ2670029.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            64991..65680
                     /gene="mutH"
                     /locus_tag="OHO85_000606"
     CDS             64991..65680
                     /gene="mutH"
                     /locus_tag="OHO85_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCQ2670030.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            65749..66462
                     /locus_tag="OHO85_000607"
     CDS             65749..66462
                     /locus_tag="OHO85_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCQ2670031.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            66600..66818
                     /gene="ygdR"
                     /locus_tag="OHO85_000608"
     CDS             66600..66818
                     /gene="ygdR"
                     /locus_tag="OHO85_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCQ2670032.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            66926..67966
                     /locus_tag="OHO85_000609"
     CDS             66926..67966
                     /locus_tag="OHO85_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCQ2670033.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(67998..69191)
                     /gene="lplT"
                     /locus_tag="OHO85_000610"
     CDS             complement(67998..69191)
                     /gene="lplT"
                     /locus_tag="OHO85_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCQ2670034.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(69184..71343)
                     /gene="aas"
                     /locus_tag="OHO85_000611"
     CDS             complement(69184..71343)
                     /gene="aas"
                     /locus_tag="OHO85_000611"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCQ2670035.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDELGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            71929..72960
                     /gene="galR"
                     /locus_tag="OHO85_000612"
     CDS             71929..72960
                     /gene="galR"
                     /locus_tag="OHO85_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCQ2670036.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTSNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(72967..74229)
                     /gene="lysA"
                     /locus_tag="OHO85_000613"
     CDS             complement(72967..74229)
                     /gene="lysA"
                     /locus_tag="OHO85_000613"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCQ2670037.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            74351..75286
                     /gene="lysR"
                     /locus_tag="OHO85_000614"
     CDS             74351..75286
                     /gene="lysR"
                     /locus_tag="OHO85_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCQ2670038.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(75273..75965)
                     /gene="ygeA"
                     /locus_tag="OHO85_000615"
     CDS             complement(75273..75965)
                     /gene="ygeA"
                     /locus_tag="OHO85_000615"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCQ2670039.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(76094..77512)
                     /gene="araE"
                     /locus_tag="OHO85_000616"
     CDS             complement(76094..77512)
                     /gene="araE"
                     /locus_tag="OHO85_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCQ2670040.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT        18988 a        20875 c        18949 g        18769 t
ORIGIN      
        1 gaggcgacaa acaatataaa tgagtagaag tctttacagg ttccacccat ttataacgct
       61 tataaatgtt taatcaggca atatttagat atttatgtgt ttgcatcgac aaacgccaat
      121 tacgcgcaat gcaggtttca atgcacaaac gtgtggcatc atccttttgg ctaatcggct
      181 gcagtgcaat gactcgcggt ttatcatcgg tcagcgttgc cagtagttca tccagtgctt
      241 caatatcgcg tacgcgcccc actggatgct tgatttcgtt ggctcgctcc agtgcctgtg
      301 acaacacttc atagccgccg cgcatgttca gctttggcga tacggtaacc caggtattcg
      361 gtgtgcagcg tacctcatga gtaccactgg tttcgatctg gcagctaaaa ccgttctttt
      421 cgagcagatc agtcagtggc agcaaatcat gaatgcaagg ctcaccaccc gtaatcacca
      481 catgccgcgc ggtgtatccc tggcgactaa tgacagccaa caagtcttca ctgctcgccg
      541 ccccccactt atcactctct ttggtcttcg ccagaatgct gaaaagggag acttcccgat
      601 cctcaagctt ttcccaggtg tgtttggtgt cgcaccaggc acagccaacc gggcatccct
      661 gtaaacgaat aaaaatggcg gggacgccgg taaagtaacc ctcaccttgc agggtctgga
      721 acatctcgtt aatcgggtac tgcatagcat tctctgtgaa gtggataatt gttaattatt
      781 gcagatcctg ccacaacaat catgtcttat taacattctg ttacaggcag atttaaagaa
      841 aaatgcgaaa catatcgtta ataattaaag ggagtaacgt attatgtcag aagaaaataa
      901 agaaaatgga tttaatcatg tcaaaacatt caccaaaatt atatttattt tttctgtatt
      961 agtttttaat gataacgaat ctaaaattac cgatgccgcc gtcaatttat ttatccagat
     1021 ttaatgagaa atttataatg cgctatttca tactaatgtt cactttcgtg tgttcctttg
     1081 ttgcagccca accaactatt gtcccacaat tacagcaaca agttacggat cttacgagta
     1141 gcttaaactc acaagaaaag aaagaactga cgcacaagtt agaatctatt ttcaataata
     1201 cccaagtgca aattgctgta ttaattgttc ccacaaccaa agacgagaca attgaacaat
     1261 atgctacaag agtttttgac aattggcgtt taggagatgc caaacgtaat gatgggatac
     1321 tgatcattgt tgcctggtca gatcgtactg tccgcatcaa ggtaggttat gggctggaag
     1381 aaaaggtaac cgatgctctg gctggagata tcatccgtag caacatgata cccgcattta
     1441 aacaacaaaa attagctcag ggattagagc tagctataaa cgctttgaat aaccaactca
     1501 cttcacaaca ccaatatccg actaatcctt cagaaagtga atcagcgtct tccagtgatc
     1561 attattactt tgctattttt tgggtatttg cagtgatgtt cttccctttc tggttttttc
     1621 atcaaggcag taatttttgt cgcgcatgta aaagtggagt ctgtatttca gcaatctatc
     1681 ttttagattt attcctgttc tccgataaaa ttttttccat tgctgtattt tccttctttt
     1741 ttactttcac catatttatg gtctttacct gtttatgtgt acttcagaaa agagcatctg
     1801 gtagaagtta tcattcagac aacagcggtt ccgctggagg ctcagatagc ggcggattta
     1861 gcggcggggg cggttcttct ggaggtggcg gcgcatccgg ccgctggtaa ctcaaccata
     1921 aaaaatgcca gcccggaggc tggcattttt aaatcagata aagtcagtct tatgcctggc
     1981 ctttgatctc tttacgaccg ttgtacggtg ctttttcgcc cagagcttct tcgatacgaa
     2041 tcagctggtt gtatttagca acacggtcag aacggctcat agaaccagtt ttgatctggc
     2101 ctgcagcagt accaacagcc aggtcagcga tggtagcgtc ttcagtttcg ccagaacggt
     2161 gagagataac tgcagtgtag ccagcatctt tcgccatctt gattgcagcc agagtttcgg
     2221 tcagagaacc gatctggttg aatttgatca ggatggagtt agcgatacct ttttcgatac
     2281 cttctttcag gatcttggtg ttggttacga acaggtcgtc accaaccagc tggattttgt
     2341 cgcccagaac tttggtctgg tatgcgaaac cgtcccagtc agattcgtcc agaccgtctt
     2401 cgatagaaac gatcgggtac tgtttggtca gttcttccag gaagtgagtg aattcttcag
     2461 aggtgaacgc tttgttgcct tcgccagcca gaacgtattt accatctttg tagaattcag
     2521 aagctgcgca gtccatcgcc agagtgatgt ctttgcccag ttcataacca gcagctttaa
     2581 cagcttcagc gataacagcc agagcttcag cgttggaacc caggttcggc gcatagccac
     2641 cttcgtcacc aacagcagtg ttcatgcctt tcgctttcag aacttttgcc aggtgatgga
     2701 aaacttcaga acccatgcgg atggcttctt tcacagtttt cgcgccaacc ggctgaatca
     2761 tgaattcctg gatatcaacg ttgttgtcag cgtgctcacc accgttgatg atgttcatca
     2821 tcggaaccgg catagagtat ttgcccggag taccgttcag ttcagcgatg tgctcgtaca
     2881 gcggcatacc tttagcagct gcagcagctt tggcgttagc cagagataca gccaggattg
     2941 cgttcgcgcc gaatttggat ttgttttcgg tgccgtccag gtcgatcatg atcttgtcaa
     3001 tgccagcctg atctttagca tctttgccaa tcagcgcctg agcgatcggg ccgtttaccg
     3061 cagcaacagc tttggttacg cctttaccca ggaaacggga tttgtcgcca tcgcgcagtt
     3121 ccagagcttc acgggaacca gtagaagcac ctgacggagc agctgccata ccgacgaaac
     3181 caccctccag atgtacttcg gcttcaacag tcgggttacc acgggagtcg atgatttcac
     3241 gaccgatgat ttttacgatt ttggacatta ggttttcctc aagtcactag ttaaactgaa
     3301 actccagaca aacaacgcgt acccagggta cgcgttgccg ctctaacttt tttacttact
     3361 tcgcctgacg tttctggaac tcgctggcgg ctttcacaaa gcctgcaaac agcgggtgac
     3421 catcacgtgg agtagaagta aactccggat ggaactggca agccacgaac cacgggtgat
     3481 tcggaacttc gatgatctcg accaactgat catccccgga acggcccgca acgcgcagac
     3541 ctgcatcttc aatctgtttc aacagcatgt tgttgacttc gtaacggtga cgatgacgct
     3601 caacaattgt cggcgcattg tacagctggc gaaccaggct atcgtcaacc aactggcact
     3661 gctgtgcgcc gagacgcatg gtaccgccga gatcgctctt ctcgctacga acttcaacgt
     3721 tgccgttttc atcgcgccac tcggtaatca gcgccacaac cgggtactta cagtctggca
     3781 caaattccgt agagttggcg ttctccatgt tggcaacatg gcgagcgtaa tcaattaacg
     3841 ccacctgcat acccaggcaa atgcccagat aaggaatatt gttctcacgc gcaaaacgcg
     3901 cggtcgtaat catgccttct acgccacgat agccgaaacc gccaggtacg aggattgcgt
     3961 ccagaccttt aaggatttca acgccgcgcg tttcaacatc ttgtgaatcg atcagtttga
     4021 tgttgacgct gacacgattc ttcagcccac cgtgtttcag tgcttcgatc actgatttat
     4081 aagcatccgg cagttcaatg tacttgccga ccataccgat ggtgacttca cttaccgggt
     4141 tcgcttcttc gaagataacc tgttcccatt cggacagatt cgcttccggg cagtttaagc
     4201 tgaatcgttt acaaatataa tcgtccagcc cctgagattt caacaggccc ggaattttat
     4261 agatggaatc gacgtctttc agagaaataa ccgctttttc cggaacatta cagaacaatg
     4321 caatcttcgc acgttcgttc gccggaacag cgcgatctga acgacaaatc aggatgtcag
     4381 gctggatacc gatggagagc agctctttta cagagtgctg agtcggtttg gttttgactt
     4441 caccagacgc tgccatgtac ggcaccagcg tcaggtgcat aaacagagtg tgctcacggc
     4501 caatttcaac agccatctgg cgaatcgctt cgaggaacgg caaggattcg atatcaccta
     4561 ctgtaccgcc gatttctacc agtactacgt catgaccttc gccaccttcc agcacgcgct
     4621 ctttgattgc gttagtgatg tgcggaataa cctgcacggt tgcgccgagg tagtcaccgc
     4681 ggcgttcttt acgcagaacg tcagagtaga tacgacccgt ggtgaagttg ttgcggcggc
     4741 tcattttggt acgaatgaaa cgctcgtagt gccccaggtc caggtcggtt tcagcgccgt
     4801 cttcagtaac gaacacttcc ccgtgttgga ttgggctcat agtacctgga tcgacgttga
     4861 tgtaaggatc cagtttcatg atggtcacat tgaggccacg ggcttcaaga atggctgcga
     4921 gggaggctgc ggcaatgcct ttacccagag aggatacgac cccgccggtc acaaaaatat
     4981 agttcgttgt catgctgaac ctgagaagtt aggttgaaaa gacgatggaa taaccaggac
     5041 gggaaagcag tatacccgaa catgacctgt gccacaaact ttcattatcc ctcctcttcg
     5101 ccagcgcact attgaaatca ggagtgagaa aatagcccct ttggggtaaa tgtttttgac
     5161 gcaaatcaag cgcttgtcat ttaaaaaatg acacaaatgg cgcttgaccg cgtaattccc
     5221 ttagagatca atttcctgcc gttttacctg ttgccagact tcttccattg tttcgaggtc
     5281 aacacctgtc atttccagtc cacgcgcggc aacaatacgc tccacttcgc gaaaacgacg
     5341 ctcaaatttt tcgttcgctt tttgcaatgc gatttccgct tttgtcccta aatgacgagc
     5401 cagattgacc gtggcaaaca gcaggtcccc catttcctcc tccagtttag cctggtcgac
     5461 aacagcctgc cgcgcttcgt acatcacctc gtcgatctct tcgtagactt tatcgactac
     5521 cggaccaagc gtcgtccaat cgaagccaac gttggcgcaa cgtttctgga ttttttgcgc
     5581 acgcattaaa gccggtaaac tacgaggaat atcgtccagc gccgaatgct gcgctttctg
     5641 cgcgcgctct tcggttttga tttgctccca acgggcaagc acttcactac tgttttcggc
     5701 agaactatca gcaaaaacat gcggatggcg acgctctaat ttatcgctaa tagcagcgca
     5761 aatatcatta aagtcaaagc gcccttcttc ctgagccatt tgcgcgtaaa acaccacctg
     5821 gaatagcaga tcgcccagtt caccgcgaag atcgtcaaaa tcttcacggg cgatggcgtc
     5881 cagcacttcg taggtttctt caagggtgta aggcgcaatg gtggcaaatg tctgctcttt
     5941 atcccacggg cagccgtttt ccggatcgcg caggcgctgc ataatagtga gcaaacggtc
     6001 gatttgattc attgaattgt cctgaaaatt gcgggtctgt caggtggaaa cctgtgacca
     6061 gaatagaagt gagttagtaa cactacccaa tcagtacgtt aattttggct ttaatgagtt
     6121 gtaattcctc tggggcaact gttcctttct tcgttgctcc tcttgcccgc caggcgatac
     6181 tttttacctg atcagctaac gctacgccat cacgttcctg accggataaa acaacttcga
     6241 acggatatcc ttttgattgc gttgtaaaag gaacacacag acacatacct gttttgttgt
     6301 tgtacatgaa aggactcagg acaacagctg gacgatgtcc agcttgctcg ctaccttttg
     6361 tcgggtcaaa atcaacccaa atcagatcgc ccatatcggg tacgtatcgg cttaccatta
     6421 ccagacttcc ttatctttcg gctctcccca gtcgatattc tcgtggaggt tttccggcgt
     6481 gatgtcgttg accagttcag caagcgtaaa tacgggctct ttacgcactg gctcaataat
     6541 taatttgcca tccaccaggt caatcttcac ttcatcatca atattcagat tgagcgcctg
     6601 cattaacgta gccgggatcc gcaccgccgg tgaatttccc caacgcttta cgctactgtg
     6661 gatcataacc ctttcctcaa accgctatca tatgtagata cagtatatat caatctacat
     6721 tgtagatacg agcaaatttc tgcctaactc ccgtgcaacc gacgcgcgtc gataacatcc
     6781 ggcacctggt tgagtttacc cagcacgcgg cccagcactt gcaggttgta aatctcaatg
     6841 gtcatgtcga tggtcgccag ttgctgtttg gtgtcgctac ggctggcaac gccaagcacg
     6901 ttcaccttct cgttggcgag aatggtcgtg atatcacgta acaacccact acgatcatta
     6961 gccaccacgc ggaccaccag cgaatatccg gcggagtagc tctcacccca taccgcgtca
     7021 acaatgcgtt ctggcgcatg ggagcgcagt tccgccagtt gttcgcaatc ggcgcggtgt
     7081 actgaaatac cgcgtccctg ggtaatgaag ccgacaatct catctccagg aatcggctgg
     7141 cagcagcgcg cgatgtggtg catcaggtta ccaacacctt caactaccac acgaccgtta
     7201 tctttactgc ggttttgcgg tgtgtagctt ttttgctgaa gctgtttcag cgcggcggcg
     7261 tcctgctctt cggcactcgg cttattaaat tgcgattgca ggaagttcac catctgattg
     7321 agacggatat ccccgccacc aatcgccgcc agcaactcgt cgacatcatt gaagttgtaa
     7381 cgcggcaaca gatgtttttc tgcttctttc aggctgatcc ccagatgttc cagctcgtcg
     7441 tcaaggattt gccgcccagc cagaatgttt ttgtcacggt cctgtttacg gaaccaggcg
     7501 tgaattttcg aacgcccacg gctggttgtg acgtaaccga ggtttgggtt taaccagtca
     7561 cggctggggt tcggctgttt ctgggtgata atttcaatct ggtcgcccat ctgcagctgg
     7621 taggtgaacg gcacaatgcg cccgccaatt tttgccccga tgcagcggtg tccgacatca
     7681 ctgtggatgt ggtaagcgaa gtccagcggc gttgatcccg caggcaaatc aacgacatca
     7741 cctttcggcg taaagacgta cacccggtcg tcaaagacct gactacgtac ttcgtcgagc
     7801 atttcgccgg aatcagccat ctcttcctgc cacgcaatca gtttacgcag ccaggcaatc
     7861 cggtcttcat gtcccgaacg tgcgccgcca gcagccgcgc cctctttata tttccagtgc
     7921 gcagcaacac ccaactctgc atcttcatgc atctgtttgg tgcggatttg gatctcaacg
     7981 gtttttccac ccggccccag aaccacggta tgaatagact gataaccgtt tggtttcggg
     8041 ttagcgacgt aatcgtcaaa ctcatccggc aggtggcgat agtgagtgtg cactatcccc
     8101 agtgcggcat aacaatcctg taaacgctcg gcgacaatac gtaccgcacg cacatcaaac
     8161 agctcatcga aggcgaggtt ctttttctgc attttacgcc agatgctgta gatgtgtttc
     8221 ggacgaccat acacttcggc tttaacgcct tcagctttca tctcagcgcg cagatgaccg
     8281 acaaactctt cgatatagtg ttcgcggtcg agacggcgtt catgcaacag ttttgcgatg
     8341 cgtttgtatt cggtcgggtg cagataacgg aagcagtaat cttccagctc ccatttcagt
     8401 tgcccaatcc caagacggtt tgccaacggc gcgtagatat tggtgcactc ttttgccgcc
     8461 agtacgcgtt catcttccgg cgcatctttt acttcccgca gatgagcaat acgctccgcc
     8521 agtttgatga ccacgcagcg aaaatcatcg accatcgcca ataacatccg gcgaacgtta
     8581 tcgacctgtt cggaggaaac agaatcagtg tgcgtcgctt tcagctggcg gatcgccgcc
     8641 atatcacgca cgccgtgaat aaggttaacg accgacttac cgacgctctc acgcagcaca
     8701 tcttcgctga ctacgttggc atccgccaga gggaaaagca gcgccgcccg cagcgtgtca
     8761 atgtccatac ttaatgtcga gaggatctcc accatctcaa caccacgcca caataacaga
     8821 ctggcatccg gatgcccctg cgtctgttgc agacaatacg cccaggtttc ggctaagcac
     8881 tcacacgact tctggctggt aatacccaga cttgcgatcc atttttccgg atcaaattca
     8941 ccagccttat tgatatgtgc acttcttacc gcaaccatcg tcctctcctt tagggaccag
     9001 acctgccgaa atcggcaaat cgcaactatt taacgcgcga gaaaagtacc atcgattcca
     9061 gatgtcccgt gtgtgggaac atatccagca tcgccagtcg cgcaatggta tatcctgctt
     9121 ttaataacgc ttcgctatcc cgagccagcg ttgcagggtt acaggataca taaactatac
     9181 gaataggttc cagttttata atttgctgca taacacctgc ggcacctgct cgcgccgggt
     9241 ccagcaacac tttatcgaag ccgtttttcg cccacggctg ctttgtgaca tcttcttcaa
     9301 gattttcgtg ataaaacgtc acattctgta agccattaag acgcgcattc tgctggcctt
     9361 tttccaccag cgccggaaca ccttctacac cgaccacact ggcagcttgt gtcgccaatg
     9421 gcagtgtaaa gttgcccata ccgcagaaca gatccagtac gcgatcttca ggttgcacat
     9481 ccagccattc caacgcacgc gctaccattt tttggttcac acccgcattg acctgaataa
     9541 aatcgcgcgg gctaaaagtt aagcgcaacc cgtttgagtc ataccagggc atctcaccag
     9601 agacggtttc gagtatctca ctatcggggg cgagatacag atccaggcct tcagaatgcg
     9661 aaaagcgttc cagtttttcg cgatctgccg aacttagcgg tgcggtatgg cgcaaaatca
     9721 tcagcgtgcc gctggttgcc tgtaccagtt caacatgacc aagatggcgc atagcttgta
     9781 agctgcccag acatgccctg actttgggca gcaatgcttc aagttggggc gctaaaatgg
     9841 ggcattgttt gacgtcgaca atgtcactgg agcccgcttt gcgaaacccc atctgaagtt
     9901 gctgtgtttt cggtaagtag ttcagactta aacgcgcgcg acggcgatag ccccagggaa
     9961 catcggcgat cacttcagag acatcgtgtt tcattaatcg ggcgagtgcc gcacttttgc
    10021 ttcgctgctg taaatccacg ctggcgtgtt gttgctgaca gccaccgcat acgccaaaat
    10081 gaggacagcg tggcgtttcg cgttccgggc tatcgcttaa ccggcgtacg actttagcgc
    10141 gggcatactg ttttttatct tcagtaacag taacttccgc gttttcctgc ggcaataatc
    10201 cggggataaa tagcgttttg ccgttatgtc gcgccacgcc ctgaccaaaa gagtcgaggt
    10261 cgttgactga aacggttatg atctgacgcg tcgtcgtgcg tcgttttgca gagtagaatt
    10321 gcgccattgg cgagactttc tcaatttaac agtgtgacct taattgtccc ataacggaac
    10381 tccatgacca actacagcct gcgcgcacgc atgatgattc tgatcctggc accgaccgtc
    10441 cttattggtt tattgctgag tatctttttc gtcgtgcatc gctataacga cttgcagcgt
    10501 caactggaag atgccggtgc cagcattatt gagccgcttg cagtttctac tgaatatggc
    10561 atgagcctgc aaaatcgcga atctatcggt cagttaataa gcgtactgca tcgtcgccat
    10621 tccgatattg ttcgcgcgat ttcggtttat gatgaaaata accgactctt tgtcacctcc
    10681 aattttcatc ttgatccctc atcaatgcag ctcggcagca acgtgccgtt tcctcgccag
    10741 ctcactgtca ctcgtgacgg cgatattatg atcctccgca cgccgattat ttctgagagt
    10801 tactcccccg acgaatcgcc cagtagcgat gccaaaaata gtcaaaatat gttgggatat
    10861 attgcgctgg agctggatct taaatcggtt cgcttgcagc aatataaaga gatctttatt
    10921 tccagcgtga tgatgctgtt ttgtatcggt attgcgctta tttttggctg gcgcttaatg
    10981 cgcgatgtaa ccggtccgat tcgcaacatg gtgaataccg tcgaccgcat ccgtcgcggg
    11041 caactcgaca gccgggtgga aggatttatg ctcggcgagc tggatatgct gaaaaacggt
    11101 atcaactcga tggcaatgtc gctggctgct tatcacgaag agatgcagca caatatcgac
    11161 caggcgacgt ccgatctgcg tgaaacgctg gagcagatgg aaattcagaa cgttgagttg
    11221 gatctggcga aaaagcgcgc ccaggaagcg gcgcgtatta aatccgagtt tctggcaaat
    11281 atgtcacacg agctgcgtac accactgaat ggtgttattg gctttacccg cctgacgctg
    11341 aaaacagaat taacaccaac gcagcgcgat cacctgaata cgattgaacg ttcggcaaat
    11401 aatttgctgg caattattaa tgatgttctc gacttctcga aactggaagc aggtaagctg
    11461 attctggaaa gtattccatt cccactacgc agcacgctgg atgaagtcgt tactctgctg
    11521 gcacattctt ctcacgataa agggttggaa ctgacgctca atattaaaag cgacgtgcct
    11581 gataacgtga tcggcgaccc gctgcgatta cagcaaatca tcactaacct ggtggggaat
    11641 gcaattaaat tcaccgagaa tggcaacatt gatattctgg tagaaaaacg tgcgctgagt
    11701 aataccaaag tgcagattga agtgcagatt cgggataccg gcattggtat tcctgaacgc
    11761 gatcaatcgc gcttattcca ggccttccga caggctgatg ccagtatttc ccgccgtcat
    11821 ggtggcaccg ggctggggct ggtgattaca caaaaactgg ttaatgaaat gggcggcgat
    11881 atttcgttcc atagccagcc gaatcgcggt tcaactttct ggttccacat taatctcgat
    11941 ctgaacccga acattattat cgaagggcca tccacccagt gcctcgcagg taagcgcctg
    12001 gcctatgtcg aaccaaactc cgcagcagcg caatgcacgc tggatatttt aagtgaaacg
    12061 ccgctggaag tggtttatag cccaacgttc tccgcgctgc ctcccgcgca ttacgacatg
    12121 atgttgttag gcatcgcggt gaccttccgc gagccgctaa caatgcaaca tgagcgatta
    12181 gcgaaagcgg tatcgatgac cgatttcctg atgctggcac ttccttgcca tgcacaagtc
    12241 aatgctgaaa aactcaagca agatggtatc ggcgcgtgtc tgctgaaacc attaacacct
    12301 acacgcctgt tgcctgccct gacggaattt tgtcatcaca aacaaaacac gcttttgcct
    12361 gtaaccgatg aaagtaagct ggcaatgaca gtcatggcgg ttgatgacaa ccccgctaac
    12421 ctgaaactta tcggcgcatt gctggaagat atggtgcaac atgtggaact ttgcgatagc
    12481 gggcatcagg cggttgaacg ggcgaaacag atgccgttcg atttgatctt aatggatatt
    12541 caaatgccgg acatggatgg cattcgggcc tgcgaactca tccaccaact cccgcatcag
    12601 caacaaacgc cggttatcgc ggtaacggcg catgcaatgg ccgggcaaaa agagaagctg
    12661 cttggcgcag ggatgagcga ttatctggcg aaaccgattg aagaagagcg attgcataat
    12721 ttgttgttgc gctacaagcc tggcagcggt atttcctctc gcgtcgtgac gcccgaagtc
    12781 aacgaaattg tggtgaaccc gaatgcgacc ctcgactggc aactggcact acgccaggca
    12841 gcaggaaaaa ccgatttagc gcgcgatatg ctgcaaatgt tactcgattt cctgcctgaa
    12901 gttcgcaaca aagttgagga acagctggtt ggagaaaacc cggaaggcct ggttgatttg
    12961 attcataaac tgcatggcag ttgcggctat agcggtgtgc cacgtatgaa gaatctctgc
    13021 caactgatcg aacaacagct acgtagcggt actaaagaag aagatttgga accggagctg
    13081 ctggaactgt tggacgagat ggataatgtc gcgcgcgaag ccagcaaaat tctcgggtaa
    13141 tggatgtctt gtcgagtagc aatcggcttt ttatgacgct ggtttcagat tggtgacaaa
    13201 gtgcctgtgt ttatgccgga tgcggcgtta acgccttatc cggcctacaa aaccaattaa
    13261 attcaatgaa ttaaaaaatg atgtagacct gatacatagc acaacaggct attttgcgtt
    13321 tagcatcagt ctcaaaccgg ctccagatag agccggtttt ggttttctgt cttaacgcac
    13381 catgcacggg cgcttgttat cgaacgtcca gccaggaatc agatactgca ttcccatcgc
    13441 atcgtcacgc gcgccaagcc cgtgtttctg atacagctca tgggctttca tcacttgatc
    13501 catatcgatt tctacaccca gccccggttt ttctggcacc tgtaccagcc cgcctttgat
    13561 ctcaaacggt tctttggtca ggcgctgatt gccttcctgc caaatccagt gcgtatcaat
    13621 agcagtaatt ttacccggtg cagcggcggc aacatgggta aacatcgcca gggaaatatc
    13681 gaagtggttg ttagagtgtg aaccccaggt caggccaaat tcatggcaca tttgcgccac
    13741 acgtaccgaa ccttgcattg tccagaaatg cggatccgcc agcgggatat caacggattg
    13801 taacgagagc gtatggccca tctgccgcca gtcggtggcg atcatattgg ttgcggttgg
    13861 caggccagtt gcgcgacgga actctgccat cacttcacgc ccggagaaac cttgctccgc
    13921 accacacgga tcttctgcat aagccagtga ccctttcagg tatttaccaa ttttaatcgc
    13981 ttcgttcagc gaccaggcac cgttaggatc gagcgtaata cgcgcctgcg ggaaacgttt
    14041 cgccagtgcc acaatagact cggcttcttc ttccccggcc agtacaccgc ccttcagttt
    14101 gaaatcgttg aagccatatt tttcatatgc cgcttccgcc aggcgcacca ccgcatccgg
    14161 cgtcatcgct tcttcatgac gcaggcgata ccagtcgcat gagtcatccg gctggctttg
    14221 atacggcagc ggtgtggctt tgcgattacc gacgaagaac agataaccga gcatttcgac
    14281 ttcgctacgc tgttgaccat cgcccagcag cgatgccacg tttaccccca gatgctgccc
    14341 cagcagatcc agcattgccg cttctatccc ggtaactaca tgaatagtgg tacgcaaatc
    14401 aaatgtctgc aaaccgcgcc caccagcatc acgatcggca aaagtattac gcaccagcgt
    14461 cagaacgttt ttgtattcac ccagcgtttt acctaccacc agcggaatcg catcttccag
    14521 cgttttacgg attttctcgc cgccgggaat ttcccctacg ccagtgtgac cagaattatc
    14581 tttgataatc acaatattac gcgtaaagaa cggtgcgtgt gcaccactca gattcatcag
    14641 catactgtca tgacccgcca ccgggatgac ctgcatttca gtaacaacag gcgtcgtaaa
    14701 ttgagaactc atatttatgt cctttttcag aatcaatgac ggccgaaaac gggacgttta
    14761 cggtcaaagg tccagccggg gatcaggtac tgcatcggac ctgcgtcgtt acgcgcaccg
    14821 ccaggcagac gtttataggc ctcatgtgcc ttttgtacct gttcccagtc cagttccacg
    14881 cccagaccgg gcgcatcagg aacggcaatt tttccgtttt taatctccag cggatttttg
    14941 gtcaggcgac aatcgccctc ctgccaaatc cagtgggtat cgatagcggt aggattaccc
    15001 ggtgccgccg cgcccacatg ggtaaacatc gccagagaga tatcgaaatg gttattagaa
    15061 tggcagcccc aggtcagccc ccagtcgtcg caaagctgcg ccacacggac tgcaccggaa
    15121 agcgtccaga agtgcggatc ggcaagtgga atatctaccg cattgagcat caccgcatga
    15181 cccatttcgc gccagttggt ggcgatcatg ttagtcgcga cgggcaagcc ggtcgcccgc
    15241 cgaaactccg ccatcacttc acgtccggag aagccctgtt ctgcgccgca tggatcttcg
    15301 gcataggtaa gaacatcatt cagccctttg cacaaagaaa tggcttcatc aagcagccag
    15361 gcaccgttgg gatcaacggt aatccgcgca tccgggaagc ttttcttcaa tgcacgaaca
    15421 gtgtcgattt cttgctcgcc aggtaacacc ccgcccttaa gtttgaaatc tttaaagccg
    15481 tagcgatcct gtgaggcttc cgccagacgc acaacggctt cgctgttcat cgctttctga
    15541 tggcgcaact gataccactc atggttgccc ggcgtatttt ccagataagg aagatcggtt
    15601 ttggtccgat caccgatata aaacagataa ccgaggacgg taatagtctc gcgttgcttg
    15661 cctggcccca acagttcgca gaccggaaca ttcagcgcct tacctagcag gtcaagcaag
    15721 gcggcttcca gcgccgccac ggcattaacg cgcaattcaa aagtccaggc acctttgccg
    15781 aaggtatcaa aatcggctgc ctgattacct ttatgcacct gctgaaccac tttattcagg
    15841 cgcgcaactt cctggcccag aaccatcgga atagcatcga ccagtgtttg ataaatcacc
    15901 tctccgcccg gcgcttcacc aatgccggta tgcccggcgt tatcggtgag taccacaata
    15961 ttgcgggtga aatatgcgtt atgtgcccca ccaatattaa gcaacatgct atcgtgcccg
    16021 gcgaccggaa tgactttcat atcagtaata acaggactgg attgtgtcgt catcttattg
    16081 ccccgcgaca ggtttcagct cgatacgctt gatatcgccc accagcacca ggtagctcag
    16141 taccgcgatt aaggcatgaa caccaacata aatcagcgcc ccattaaagg agccagtcgt
    16201 gccaacgata taaccaattg cgattggcgt gacgatgcca gaaatgttac cgaacatgtt
    16261 gaacaggcca ccggaaagac cgctgatctc ttttggcgcg gtatctgcca ttactgccca
    16321 acccagcgcc ccgatgccct taccgaagaa ggccagcgcc ataaagccga tgatcatcca
    16381 ctcaacgttg acgtagttgc agaacaccat caccatcgac agcaacatgc ccattacgat
    16441 cggtgtttta cgcgcaatgt tcagcgatcc cgtgcggcgc atcagccagt cggaaataat
    16501 cccacccagc acaccgccga taaaaccgca aaccgccgga acggaagcca caaagcccgc
    16561 tttcagaatc gacatcccgc gtgcctgcac cagataaacc gggaaccagg taataaagaa
    16621 gtaagtcagg gcgttgatac agtactgacc gatataaacg ccgatcatca tccgtgaccc
    16681 gagcagctgt ttgatctgcc cccacttcac gctgaacggc actttaactt tagtgttttg
    16741 ctgatccata ttgatcagcg cgccacccgc ggcgatatac tccagctctt tctgatttac
    16801 gcccggatgt tgatttggct cgtggatgac tttcaaccag ataaagctga tgacaatgcc
    16861 cagaccgccc ataaagaaga agacgtgtga ccagcccact tcatgcgtca gccagcccat
    16921 aatcggcgcg aagataaccg ttgcgaagta ttgggcggag ttaaaaatcg acaccgccgt
    16981 tcccctttcc tgcgccggaa accaggccgc aacaatgcga ctgttgccgg ggaaagatgg
    17041 cgcttcagca agcccgacca ggaagcgcag cgtaaacagg gcaacgataa tgccgaatcc
    17101 actaaagata tcgacgaagc cttgcagcaa ggtaaacatc gaccagataa agatcgacca
    17161 gaagtagacg cgttttgaac caaaacggtc cagcaaccag ccacctggga tctgcccgat
    17221 aacataagcc catgagaagg cagagaacac atagcccatt cccacgggat caaggccgat
    17281 atctttggcc atttccgaac cggcaataga aagcgtagcg cggtcgccgt agttgaagga
    17341 tgtgacgata aacaacatca ccactatcca gtaacgagca ttcgtgcatt tttccacact
    17401 gctcgcagcc tgacttaaag aactcattgt tgcactcctg aaaattcgcg ttagccacgc
    17461 tcactctgga ctgcgacata gccaggaaat cagaggtgac gtagggtgtt ttttgccgtt
    17521 tttataggtc gttcgccgaa tacggcgcgt gtttatatct ggcaatagca gtataaaaag
    17581 cgcgccatag cggctcaccg tgcaacaaca caacattaat gcgttcaatg aggcccgatt
    17641 ttggcattag ccctggacgg tggaatccac ttcacggaaa tgaaaacaag aacaagaaag
    17701 gaagggttaa aacgaagaaa taaagaagag tatgaaatgg atcgcttgac tccaggcaaa
    17761 cgccagtaaa aatccgcgct atgaagcagt ttttactggc atttgcctga aaagattcga
    17821 ttcagcaccg ctaaaacgac atttaccgct cgctgaacat atcaggacaa cagcgtgccc
    17881 cactgttcga cccacggatt tgattccgtt tccggttccg ggttctcact ggcgtcaatc
    17941 aacagtattt cgccaacccg ctgagcgctc tgttcctgca acaaggcatc gaactgtttg
    18001 ccgccattgc agaaatttac ataactgctg tcgccgagcg caatcacgcc ataacgcaga
    18061 ttcggctgga agcccagact atctttgatt ccctgaaaga gcggcacaat gctatcagga
    18121 aggtcgccct gcccggtcgt ggacgtaacc accagaacat acttatcctg ataaggcagc
    18181 cagtcgctta attcaggatc ttcaaatacc gttgctttgt ggccctgtgc ggtcagaatt
    18241 gcttctgctt cttccgccac taacagcgaa tttccgtaca tggtgccgac aaaaatacca
    18301 atttccgcca taccgttttc tccctggatt aggaacttat ctctccatcc tgacccgatg
    18361 gcgcactgaa ctcaaccctt tcattttcag gaagcagacc gcgccagcca aattgtgata
    18421 acgcctgcat ccaggtgtcg tccaggcccg cgtgaatagt cagcggctcg ccagtaaaag
    18481 gatgcgtcaa tgacaactga ctggcatgta gcattaaccg ctggaggcca aaatgctcag
    18541 caccgctgcg attctggcgt aaatcgccat gtttgctatc gccaataatc ggatgacgca
    18601 aatgggcaag atgtcggcga agctgatgtt tgcgtccggt tttcggctcc agttccacca
    18661 ggccgtagcg cgtggtcggg taacgtccgg tcgctaccgg catttctacg gtcgccagac
    18721 cgcgataatg cgtcactgct ggctgcgagc ctttatcttc gcgggcaaat ttatcagcga
    18781 ttttgtccag ttcttccacc agtggataat ccagcaccgc ttcttccatc aaccagccgc
    18841 gcacaatcgc atggtaacgt ttctggattt ggtgctgttc aaactgttgt gccagcagcc
    18901 gtccggcctc gctggataat cccatcaaca acacaccaga agtgggtcgg tccagacgat
    18961 gagcagtaaa aacatgctgt cctatctggt cacgcacggt ttgcatgacc actactttct
    19021 cgtcgcgatc cagccagcta cggtgaacca gccagccgga gggtttattt accgcaacca
    19081 gccattcatc ctgatagagt atttccagca ttagctcgca tcatccgcaa aaagagcatc
    19141 cagtttttcc aactcagcca gaataagcgc gcgttgcgga tggtccgtcg ccagcgccat
    19201 ttcataatag ggtgcaacag caaaagcgcc cggtaacggc tggttgttat ttaacaaatc
    19261 gtgcattcgc gggatcagca cccactgcaa ccactccagt ggttccatgg tgtccataaa
    19321 gaacggttgg gtactattaa attgatgcgg ctggggttca tcgtttcgcc agtgctgatg
    19381 ttcacgcagt aacgcttcaa gcgcctgcaa ctggagacga acgcggtcat gagtggtcat
    19441 aaagcaacct caataagaaa aactgccgcg aagaatagca ggaataaaaa aagggagcac
    19501 tgtattcaca gcgctcccgg ttcgtttcgc agcattccag ctacttttgt tgctccctgc
    19561 tcatccttga caacttttcc tctggccttg cggccaatcg ttcatcctga actattgctt
    19621 cctgctcaca ccaccccgat gtgatacttc atcctgaagt gtccctggcc ttcctgaccc
    19681 accgaatcat cctgaccggt tctcattctc catcctggag gtgtccttta acgcgtcctg
    19741 cgtcatcctc ttcgcttcat ccagaaacct ttccctgaaa caccatcctg gtgtgtcctg
    19801 cagaagtgtc atcatcctga tgttcacttc gttgtctgac tccctgtcga cgaagataga
    19861 atcgtctttt tcgcgaagtc ttacaaggcg cttacagaca atgccttaag gaaaatttca
    19921 tatgaaagtt aagaattaac aatcaacatt ttgattttaa atgaaaataa cgattcttat
    19981 ctttttaagt attcgcttat ttcctggcga tctcctacag agtctgtaag agatctctca
    20041 caaccagatg gggtatttgg attacagaag aggcttaagt tgattaagga attccgcaag
    20101 atttgaggct aaatgcgtcc gtttacgtgt tcccagcgtc tctttgcaca cttcaccgga
    20161 gagattacac accgagatga cctctaactc ttcttccagc gtcgcgataa atagcgtggg
    20221 cggcagtttt agccttttct gagtcaccag atgaccaatc agattttcct gcacccgacg
    20281 gaagtcgtct tcactccagg tttgcagcaa tgtcagtttg atatcgccaa actgcgcgtg
    20341 catatccccg gcaaactgag tcgtataaaa ggtatgaatt gtgggttgta tcacaatatc
    20401 aaaagcgcgt tcaaccgcgt ttacattttg ttccccggta aacggctggg gttgccagta
    20461 tacggcatct tcggtagttg aaataatgca cggtgaagga acaccgtaca gttcctcact
    20521 taacggccag cttttatgct cttcatgcca cgcatcgcag tagcgtgcag taaaatcttt
    20581 cagggcttgt gcggtcaaat cgtccactaa tttctctctt cacttaaacc agatacactt
    20641 gtcgtttagt ttatctggtt tatgacggtg aaacatgtct tcttatgcaa accatcaggc
    20701 acttgcgggc ctgactcttg gaaaatcaac cgattaccgg gatacctatg acgccagcct
    20761 actgcaaggc gttccacgca gcctgaatcg cgacccgctg ggtctgaaag cggataacct
    20821 gccttttcac ggtacggata tctggacgct gtatgaactt tcctggctga atgcgaaagg
    20881 tttgccgcag gttgccgtcg gtcacgttga acttgattac accagcgcaa atctgattga
    20941 gtcgaagagt tttaagctct atctcaacag ttttaaccag acgcgtttta ataactggga
    21001 tgaggtgcgc cagacgctgg agcgcgactt aagcacttgc gcccagggta aggttagcgt
    21061 ggcgttatat cgtcttgatg aactggaagg ccagccgata ggtcatttta atggcacttg
    21121 cattgatgac caggatatca ctatcgataa ctatgaattc actactgact atctggagaa
    21181 tgccacctgt ggtgaaaaag tagtggaaga gacgcttgtc agccacctgc tgaaatcaaa
    21241 ctgcctgatc acccatcaac cagattgggg ttcgatccaa attcagtatc gtggacgcca
    21301 aattgacaga gaaaaactgc tgcgttacct ggtctcattc cgtcatcaca acgagttcca
    21361 cgaacagtgc gtggaacgca tctttaatga cctgttacgc ttctgccagc cagaaaaatt
    21421 gagcgtttac gcacgttata cccgtcgtgg cggtctggac attaacccgt ggcgcagtaa
    21481 tagcgatttt gtcccatcga ccacaagact ggttcggcaa taaatttttt ctcaattttg
    21541 cgtgctggat tcacgcagaa ggttgtgaaa ggtcatcagg cagggctatt gtaatcaaag
    21601 ggaatgacga tattcgtccc ataaggagtt tttcttgatt acacatatta gcccgcttgg
    21661 ctccatggat atgttgtcgc agctggaagt ggatatgctt aaacgcaccg ccagcagcga
    21721 cctctatcaa ctgtttcgca actgttcact tgccgtactg aactccggta gtttgaccga
    21781 taacagcaaa gaattgctgt ctcgttttga aaatttcgat attaacgtcc tgcgccgtga
    21841 acgcggcgta aagctggaac tgattaatcc cccggaagag gcttttgtcg atgggcgaat
    21901 tattcgcgct ttgcaggcca acttgttcgc ggttctgcga gacattctct tcgtttacgg
    21961 gcaaatccat aacaccgttc gttttcccaa cctgaatctc gacaactccg tccacatcac
    22021 taacctggtc ttttccatct tgcgtaacgc tcgcgcgctg catgtgggtg aagcgccaaa
    22081 tatggtggtc tgctggggcg gtcactcaat taacgaaaat gagtatttgt atgcccgtcg
    22141 cgtcggaaac cagctgggcc tgcgtgagct gaatatctgc accggctgtg gtccgggagc
    22201 gatggaagcg ccgatgaaag gtgctgcggt cggacacgcg cagcagcgtt acaaagacag
    22261 tcgttttatt ggtatgacag agccgtcgat tatcgccgct gaaccgccta acccgctggt
    22321 caacgaattg atcatcatgc cggatatcga aaaacgtctg gaagcgtttg tccgtatcgc
    22381 tcacggcatc attatcttcc ctggcggtgt gggtacggca gaagagttgc tgtatttgct
    22441 gggaatttta atgaacccgg ccaacaaaga tcaggtttta ccattgatcc tcaccggccc
    22501 gaaagagagc gccgactact tccgcgtact ggacgagttt gtcgtacata cgctgggcga
    22561 aaacgcgcgc cgccattacc gcatcatcat tgatgacgcc gctgaagtcg ctcgtcagat
    22621 gaaaaaatcg atgccgctgg tgaaagaaaa tcgccgcgac actggtgacg cttacagctt
    22681 taactggtca atgcgcattg caccagattt gcaaatgccg tttgagccgt ctcacgagaa
    22741 tatggctaat ctgaagcttt acccggatca acctgttgaa gtgctggctg ccgacctgcg
    22801 ccgtgcgttc tccggtattg tggcgggtaa cgtaaaagaa gtcggtattc gcgccattga
    22861 agagtttggt ccttacaaaa tcaacggcga taaagagatt atgcgtcgta tggacgacct
    22921 gctacagggt tttgttgccc agcatcgtat gaagttgcca ggctcagcct acatcccttg
    22981 ctacgaaatc tgcacgtaat ctccgctccc ccggtgacgt ttgttgccgg gttttctcgt
    23041 ttttggtcac ttactcatca actcatttca tttgttatat gaatgtttct taccaccctc
    23101 atgcgacaaa tatcatcaca gttaatatgt catacaattt atattgcaac gcaaacgttt
    23161 ccctattttc ataaaccgta ttttatcgct atgaaaaaga aattatcgcc atgattaact
    23221 aaaagtattg attttttcag ttcaacctac atatattgcg cgccccggaa gaagtcagat
    23281 gtcgtttaat gggcaaatat tgcccttaaa ttctctttta cttttgattt acagagtaaa
    23341 gcgttgggat aatctatctt ccaagtagat tattgtattt gggatcaaga tcactgatag
    23401 atacataact tgtgtgtatc tttccgccct caaattatta cggcggtaaa tgattaagcc
    23461 atcgccgata gacagatttc atttttacgg tcaggcacct tcccgggctg aactggctaa
    23521 aagctgaatt atttgcattc ctccaggaga aatagatgga aacgactcaa accagcacga
    23581 ttgcgtcgaa agactctcgt agtgcctggc gcaagacaga caccatgtgg atgctgggcc
    23641 tttacggcac ggcaatcggc gcgggcgtgc tgttcctgcc aatcaacgcc ggtgttggcg
    23701 gtatgatccc gctgatcatc atggctatcc ttgcgttccc gatgacgttt tttgctcacc
    23761 gcggcctgac tcgcttcgta ctgtctggta aaaacccggg cgaagacatc accgaggttg
    23821 tagaagaaca ctttggtatt ggcgcaggta aactgattac cctgctctac ttcttcgcta
    23881 tctacccgat cctgctggtt tatagcgtgg caatcaccaa taccgttgaa agcttcatgt
    23941 ctcaccagct gggtatgacg ccaccgccgc gtgcgattct gtcgctgatc ctgatcgtgg
    24001 gtatgatgac catcgttcgc ttcggtgagc agatgatcgt taaagcgatg agtattctgg
    24061 tattcccgtt tgttggcgta ctgatgctgc tggctctgta cctcatcccg cagtggaacg
    24121 gcgcagcact ggaaacgctg tctctggaca ctgcatctgc aaccggaaac ggtctgtgga
    24181 tgaccctgtg gctggcaatt ccggtaatgg tgttctcgtt caaccactct ccgatcatct
    24241 cttctttcgc cgttgcgaag cgtgaagagt acggcgatat ggcagaacag aaatgctcga
    24301 agatcctggc attcgcacac atcatgatgg tgctgaccgt aatgttcttc gtcttcagct
    24361 gcgtactgag cctgactccg gcagacctgg ctgcggctaa agagcagaac atctcgattc
    24421 tgtcttacct ggctaaccac tttaacgcac cggttatcgc gtggatggct ccgattatcg
    24481 cgattatcgc tatcaccaaa tccttcctcg gccactacct gggcgcacgt gaaggcttca
    24541 acggtatggt gattaaatct ctgcgtggta aaggtaagtc tatcgaaatc aacaagctga
    24601 accgtatcac tgcgctgttc atgctggtaa cgacctggat tgttgccacc ctgaacccga
    24661 gcatcctggg tatgattgaa accctgggcg gcccaatcat cgcgatgatc ctgttcctga
    24721 tgccgatgta cgcaattcag aaagtaccgg caatgcgtaa gtacagcggt cacatcagca
    24781 acgtattcgt tgtcgtgatg ggtctgattg caatctccgc aatcttctac tctctgttca
    24841 gctaagtcct ttcgcgccgc tttcgggcgg cgcttcctcc gttttaacgc gatgtatttc
    24901 ctatgattag cgtattcgat attttcaaaa tcggcattgg cccttccagt tctcataccg
    24961 ttggaccaat gaaagcgggt aaacaattta ccgacgatct gattgcccgt aatctgctta
    25021 aagacgtgac ccgcgtggtg gttgacgtgt acggctcgct ctctctgacc ggtaaaggcc
    25081 accacactga tatcgccatt attatgggcc tggcgggtaa cctgccggat accgtggata
    25141 tcgattccat ccctggtttt attcaggatg tgaatactca tggtcgcctg atgctggcaa
    25201 acggtcagca tgaagtggag ttcccggttg atcagtgcat gaacttccat gcagacaacc
    25261 tttctctgca tgaaaacggt atgcgcatta ccgcgctggc gggcgataaa gtcgtttaca
    25321 gccagactta ctactctatt ggcggtggct ttatcgttga tgaagagcat tttggccagc
    25381 agaatagcgc accggttgaa gttccttatc cgtacagttc agcagccgat ctgcaaaaac
    25441 attgtcagga aaccgggctg tcactctctg gcctgatgat gaaaaatgag ctggcgctgc
    25501 acagcaaaga agagctggaa cagcacctgg cgaacgtatg ggaagtcatg cgtggcggta
    25561 ttgagcgcgg tatttccacc gaaggcgtgt tgcctggcaa actgcgcgtt ccacgccgtg
    25621 ctgcggcact acgccggatg ctggtcagcc aggataaaac caccactgac ccgatggcgg
    25681 ttgttgactg gatcaacatg tttgcattgg cagtgaacga agagaacgct gctggcggac
    25741 gcgtggtgac tgcgccgact aacggtgcgt gcgggattat cccggcagtg ctggcgtact
    25801 acgacaagtt tatccgcgaa gtgaacgcta actcactggc tcgttacctg ctggtagcca
    25861 gtgccattgg ttctctttat aagatgaacg cgtcaatttc tggtgctgaa gtcggctgcc
    25921 agggtgaagt tggtgtggcg tgctcaatgg cggcggctgg tctggcagaa ctgttaggtg
    25981 caagcccggc gcaggtgtgc atcgcggcgg aaatcgccat ggagcacaac ctcggtctga
    26041 cgtgtgaccc ggtcgccgga caggtccagg tgccatgcat cgagcgtaac gccattgcgg
    26101 cagtaaaagc ggtgaacgcc gcacgtatgg cgctacgccg taccagcgag ccgcgcgtct
    26161 gcctcgataa agttatcgaa accatgtacg aaacaggtaa agatatgaac gccaagtacc
    26221 gcgaaacctc tcgcggcggc ctggcaatga agatcgttgc ctgcgattaa tcgctctcca
    26281 gaggcctcgt tttgcgaggc ctcttcccga tttctcatcc agccgtagcc tgttccggca
    26341 tcgaatgtta cccttatcgc ctgatcttta agggggttat cgtggctgtt catttgctta
    26401 ttgtcgatgc actgaatctt attcgtcgca ttcatgccgt tcagggatcg ccctgtgtcg
    26461 aaacctgcca gcatgcgctc gatcagctca ttatgcacag ccagccaacc cacgcggtcg
    26521 ccgtttttga tgatgaaaac cgcagtagcg gctggcgtca tcagcgttta ccagattaca
    26581 aagcgggtcg accgccaatg ccggaagagt tgcacgacga gatgcctgca ttacgcgccg
    26641 cctttgagca acgcggcgtc ccgtgctggt caaccagcgg caacgaagcc gatgacttag
    26701 ccgccacgct ggcggtcaaa gtgacacagg ccgggcatca ggcaacgatt gtttcgacag
    26761 ataaaggcta ctgtcagtta ctttcaccga cattacgtat tcgtgattac ttccagaaac
    26821 gttggctgga tgcgccattt atcgataaag aatttggcgt tcaaccgcag cagttgcccg
    26881 attactgggg acttgcgggg atcagcagtt caaaggtacc gggtgttgcg ggaatcggac
    26941 caaaaagcgc cacgcagctg ctggtcgagt ttcagagtct ggaagggata tatgagaatc
    27001 tggatgcggt tgccgaaaag tggcgcaaaa aattagaaac ccataaagag atggcgtttc
    27061 tgtgccgcga tattgcccgc ttacaaaccg atttgcatat cgacggcaat ttacagcaat
    27121 tgcggttggt acggtaacgg cgagccggat acgccgcaaa cgtcgtatcc ggcattatca
    27181 catcagcgca tttaccaggc ggtatggtaa agctctacaa tatcctcaag cgttgcttca
    27241 cgcgggttgc caccggtaca aacatcatcc agtgccgcct gcgccagtgc cggaatgtct
    27301 tccttgcgta caccaacatc acgcaaatgt ggcggaatac cgacatcacg gttgagagca
    27361 aacaccgctt caacagcggc attacgcgcc tcttccaggc tcataccttc cactttcacg
    27421 cccataacgc gcgcgatatc gcggtacttc tcaccggtaa agtcagcgtt atagcgcatg
    27481 acatgcggta gcaggatggc gtttgcaaca ccgtgtggag tgttataaaa cgcgcccagt
    27541 ggatgcgcca taccatgcac caaccctaac ccaacattcg agaagcccat acccgcaaca
    27601 tactgcccga gcgccatttc ttctccggca tccttatcac cagcaaccga tcctcgcagc
    27661 gccccagcaa tgatttcaat cgctttaatg tgcagtgcat cggttagcgc ccacgcgcca
    27721 cgggtaatat acccctcaat agcatgagtg agcgcatcga cacccgtcgc agctttcagc
    27781 gctggaggca taccatccat catgtcagcg tcaataaacg ccacctgcgg gatatcatgc
    27841 ggatcaacgc aaacaaactt gcgccgtttt tcttcgtcag tgatcacgta gttaatggtc
    27901 acttctgccg cagtgcctgc tgtggtgggg attgccagaa tcggtacact gggtttattg
    27961 gtcggggaaa gtccttccag gctacgcaca tcggcaaact ccgggttgtt gctgataatg
    28021 ccaatcgctt tacaagtatc ctgtggagaa ccaccaccaa tagcgatcag gtaatccgcg
    28081 ccgctattct ggaatacacc gagcccttct ttgacgacag taattgttgg gttgggcact
    28141 acgccgtcgt aaatcgccca tgccagccct gcagcatcca tcttatcggt cactttcgcc
    28201 accacgccgc attgcaccag cgttttatcg gtgacgatca gcgccttctg ataaccacgg
    28261 cgtttcacct catcggttaa agccccaaca gcaccccgac caaaccatgc cgtttcgttc
    28321 agaatcattc tgttagccat catccttctc cttgttgctt tacgaaatta ctcttcaatt
    28381 cgtaacccat aggttttgaa tttctccagc actacggcaa tctcttcatc gctcagcact
    28441 ggcaccgggt ccgtaatcgc cagggtcgtc aggtaaagtt gcgccagcac ttcaacttca
    28501 tgcgccagcc ataacgcttt ttccagattc acctcacaag cgataagccc atgatgttgt
    28561 aacaaagttg ccttacgatt tttgagagcc agcgcaacat gttcagaaag ttcgcgtgtt
    28621 ccaaaggtcg cataaggcgc gcaaggaata gaattaccac ctgccgccgc aatcatgtag
    28681 tgaatagcgg ggatcggtcg gttaagaatg gaaactgctg tgcaatgaac ggcatgattg
    28741 tgaacaaccg cgttggcatc cggtctgctt tgataggccg ccatatggaa acgccattcg
    28801 cttgagggga gctttccttc ctcatgttta ccgttgccat caataaagac aatatgcgac
    28861 tccgtcagtt tttcatatgg aatgcctgta ggcgtaatca gcatcccatc ctgataacgt
    28921 acactgacgt tccccgctgt cccctggttc agtcccaggc gggtcatttc caggcaagtg
    28981 tcaataatct gacgagcaag tttatttcgt tccattagct acctctctct gattcaaaac
    29041 agggcaataa tgttgttcct ttcacactat tgaattagcc gtttaattac ccaccatctt
    29101 cttcctgatt aacaagaaag aaattcacaa gcttatattt tgtgacctgg ttcaactaat
    29161 cacagtaaat aactgcaagt tctcttttta taaccccatt aaaaatgacc gctcttaaaa
    29221 atatttatca aaacggtcat ttttctattc acccaagcca ggaatgcccg ttttcggcat
    29281 aaacaattaa tacggtcatc tggtttgtgt tttttatgat ttattttctg aaacgggcat
    29341 gaaatttcga ttattaaagt gatggtagtc acataaagtc accttccagc taataagtgt
    29401 gaccgccgtc atattacaga gcgtttttta tttgaaaatg aatccatgag ttcatttcag
    29461 acaggcaaat attcactgat atgaagcccg aactcgctgg ttttgcactt ttgaaaacat
    29521 aaccgattac gtgcttaagc ttctgaacct aagaggatgc tatgggaaac acatcaatac
    29581 aaacgcagag ttaccgtgcg gtagataaag atgcagggca aagcagaagt tacattattc
    29641 cattcgcgct gctgtgctca ctgttttttc tttgggcggt agccaataac cttaacgaca
    29701 ttttattacc tcaattccag caggctttta cgctgacaaa tttccaggct ggcctgatcc
    29761 aatcggcctt ttactttggt tatttcatta tcccaatccc tgctgggata ttgatgaaaa
    29821 aactcagtta taaagcaggg attattaccg gattattttt atatgccttg ggcgctgcat
    29881 tattctggcc cgctgcagaa ataatgaact acaccttatt tttagttggc ctatttatta
    29941 ttgcagccgg attaggttgt ctggaaactg ccgcaaaccc ttttgttacg gtattagggc
    30001 cagaaagcag tggtcacttc cgcttaaatc ttgcgcaaac atttaactcg tttggcgcaa
    30061 ttatcgcggt tgtctttggg caaagtctta ttttgtctaa cgtgccacat caatcgcaag
    30121 acgttctcga taaaatgtct ccagagcaat tgagtgcgta taaacacagc ctggtattat
    30181 cggtacagac accttatatg atcatcgtgg ctatcgtgtt actggtcgcc ctgctgatca
    30241 tgctgacgaa attcccggca ttgcagagtg ataatcacag tgacgccaaa caaggatcgt
    30301 tctccgcatc gctttctcgt ctggcgcgta ttcgtcactg gcgctgggcg gtattagcgc
    30361 aattctgcta tgtcggcgca caaacggcct gctggagcta tttgattcgc tacgctgtag
    30421 aagaaattcc aggtatgact gccggctttg ccgctaacta tttaaccgga accatggtgt
    30481 gcttctttat tggtcgtttc accggtacct ggctcatcag tcgcttcgca ccacacaaag
    30541 tcctggccgc ctacgcatta atcgctatgg cactgtgcct gatctcagcc ttcgctggcg
    30601 gtcatgtggg cttaatagcc ctgactttat gcagcgcctt tatgtcgatt cagtacccaa
    30661 caatcttctc gctgggcatt aagaatctcg gccaggacac caaatacggt tcgtccttca
    30721 tcgttatgac catcattggc ggcggtattg tcactccggt catgggtttt gtcagtgacg
    30781 cggcgggcaa catccccact gctgaactga tccccgcact ctgcttcgcg gtcatcttta
    30841 tctttgcccg tttccgttct caaacggcaa ctaactgaac atattttccg aataaagtga
    30901 ggaatctgta atgaaaaaaa tcagcttacc gaaaattggt atccgcccgg ttattgacgg
    30961 tcgtcgcatg ggtgttcgtg agtcgcttga agaacaaaca atgaatatgg cgaaagctac
    31021 ggccgcactg ctgaccgaga aactgcgcca tgcctgcgga gctgccgtcg agtgtgtcat
    31081 ttccgatacc tgtatcgcgg gtatggctga agccgctgct tgcgaagaaa aattcagcag
    31141 ccagaatgta ggcctcacca ttacggtaac gccttgctgg tgctatggca gtgaaaccat
    31201 cgacatggat ccaacccgcc cgaaagccat ttggggcttt aacggcactg aacgtcccgg
    31261 cgctgtttac ctggcagcgg ctctggcagc tcacagccag aaaggcatcc cggcattctc
    31321 catttacggt catgacgttc aggatgccga tgacacatcg attcctgccg atgttgaaga
    31381 aaaactgctg cgctttgccc gcgccggttt ggccgtcgcc agcatgaaag gtaaaagcta
    31441 tctgtcgctg ggcggcgttt cgatgggtat cgccggttcc atcgttgatc acaacttctt
    31501 tgaatcctgg ctgggaatga aagtccaggc ggtggatatg accgaactgc gtcgccgtat
    31561 cgatcagaag atttacgacg aagccgaact ggaaatggca ctggcctggg ctgataaaaa
    31621 cttccgctat ggcgaagatg aaaataacaa acagtatcaa cgcaatgccg agcaaagccg
    31681 cgcagttctg cgcgaaagtt tactgatggc gatgtgtatc cgcgacatga tgcaaggcaa
    31741 cagcaaactg gccgatattg gtcgcgtgga agaatcactt ggctacaacg ccatcgctgc
    31801 gggcttccag gggcaacgtc actggaccga tcaatatccc aatggtgaca ccgccgaagc
    31861 gatcctcaac agttcatttg actggaatgg cgtgcgcgaa ccctttgtcg tggcgaccga
    31921 aaacgacagt cttaacggcg tggcaatgct aatgggtcac cagctcaccg gcaccgctca
    31981 ggtatttgcc gatgtgcgta cctactggtc accagaagca attgagcgtg taacggggca
    32041 taaactcgat ggactggcag aacacggcat catccatttg atcaactcag gttctgctgc
    32101 gctggacggt tcctgtaaac aacgcgacag cgaaggtaac ccgacgatga agccgcactg
    32161 ggaaatctct cagcaagagg ctgacgcttg cctcgccgct accgaatggt gcccggcgat
    32221 ccacgaatac ttccgtggcg gcggttactc ttcccgcttc cttaccgaag gcggcgtccc
    32281 gttcaccatg actcgtgtca acatcatcaa aggcctggga ccggtactgc aaatcgcgga
    32341 aggctggagc gtggaattgc cgaaggatgt gcatgacatc ctcaacaaac gcaccaactc
    32401 aacctggcca accacctggt ttgcaccgcg cctcaccggt aaagggccgt ttacggatgt
    32461 gtactcggta atggcgaact ggggcgctaa ccatggggtt ctgaccatcg gccacgttgg
    32521 cgctgacttt atcactctcg cctccatgct gcgtatcccg gtatgtatgc acaacgttga
    32581 agagaccaaa gtgtatcgtc cttctgcctg ggctgcgcac ggcatggata ttgaaggcca
    32641 ggattaccgc gcttgccaga actacggccc gttgtacaag cgttaataca ttactccctg
    32701 atgtgatgcc cggtcgctcc ggctaccggg cctgaacaag caagagtggt tagccggata
    32761 agcaatgtta tccggctata ttgcaggagc gattatgaaa caagaagtta tcctggtact
    32821 cgactgtggc gcgaccaatg tcagggccat cgcggttaat cggcagggaa aaattgttgc
    32881 ccgcgcctca acgcctaatg ccagcgatat cgcgatggaa aacaacacct ggcaccagtg
    32941 gtctttagac gccattttgc aacgctttgc tgattgctgt cggcaaatca atagtgaact
    33001 gactgaatgc cacatccgcg gtatcgccgt caccaccttt ggtgtggatg gcgctctggt
    33061 agataagcaa ggcaatctgc tctatccgat cattagctgg aaatgtccgc gaacagcagc
    33121 ggttatggac aatattgaac agttgatctc cgcacagcgg ttgcaggcta tttctggcgt
    33181 cggagccttt agtttcaata cgttatataa gttggtgtgg ttgaaagaaa atcatccaca
    33241 actgctggaa cgcgcgcacg cctggctctt tatttcgtcg ctgattaacc accgtttaac
    33301 cggcgaattc actactgata tcacgatggc cggaaccagc cagatgctgg atatccagca
    33361 acgcgatttc agtccgcaaa ttttacaagc caccggtatt ccacgccgac tcttccctcg
    33421 tctggtggaa gcgggtgaac agattggtac gctacagaac agcgccgcag caatgctcgg
    33481 cttacccgtt ggcataccgg tgatttccgc aggtcacgat acccagttcg ccctttttgg
    33541 cgctggtgcc gaccaaaatg aacccgtgct ctcttccggt acatgggaaa ttttaatggt
    33601 ccgtagcgcc caggttgata cttcgctgtt aagtcagtac gccggttcca cctgcgaact
    33661 ggatagccag gcagggttgt ataacccagg tatgcaatgg ctggcatccg gcgtgctgga
    33721 atgggtgaga aaactgttct ggacggctga aacgccctgg caaatgttga ttgaagaagc
    33781 tcgtctgatc gcgcctggcg cggatggcgt aaaaatgcag tgtgatttat tgtcgtgtca
    33841 gaacgctggc tggcaaggag tgacgcttaa taccacgcgg gggcatttct atcgcgcggc
    33901 gctggaaggg ttaactgcgc aattacagcg caatctacag atgctggaaa aaatcgggca
    33961 ctttaaggcc tctgaattat tgttagtcgg tggaggaagt cgcaacacat tgtggaatca
    34021 gattaaagcc aatatgcttg atattccggt aaaagttctc gacgacgccg aaacgaccgt
    34081 cgcaggagct gcgctgttcg gttggtatgg cgtaggggaa ttcaacagcc cggaagaagc
    34141 ccgtgcacag attcattatc agtaccgtta tttctacccg caaactgaac ctgaatttat
    34201 agaggaagtg tgaaatgctg aaaacaattt cgccgttaat ttctcccgaa ctattgaaag
    34261 tgctggcaga gatgggacat ggagatgaaa ttattttttc cgatgctcac tttcccgccc
    34321 attcgatggg accgcaggtg atccgcgctg atggcctgtt ggtgagcgac ttgctccagg
    34381 cgattatccc gttatttgag ctggacagtt atgccccgcc gctggtgatg atggcagcgg
    34441 tagaaggtga cactctcgat cctgaagtag aacgacgtta ccgtaatgcg ctttcactac
    34501 aagccccgtg tcctgacatc atccgcatca atcgttttgc gttttatgaa cgggcgcaaa
    34561 aagcctttgc gatcgttatc acaggcgaac gagcgaagta cgggaatatt cttttaaaaa
    34621 aaggggtaac accgtaatct cataccggta cgcccgcatg acgcgggcgg ttatcgaatg
    34681 atggggtgaa aaatatgaaa gcggcacgcc agcaagcgat agtcgacctg ctgctgaacc
    34741 ataccagcct gaccacggaa gctctctctg aacagctaaa ggtcagtaaa gaaaccattc
    34801 gtcgcgatct caatgaatta cagacgcagg gtaaaattct gcgcaatcat ggacgcgcta
    34861 aatatatcca ccgtcaaaat caagacagtg gcgatccctt tcacatccgg ctgaaaagcc
    34921 attatgcgca taaagcagat atcgcgcgcg aggcgctcgc gtggattgaa gaagggatgg
    34981 tgatagcctt agacgccagt tcaacttgct ggtatctggc acgccagttg cctgacatca
    35041 acattcaggt cttcaccaat agccatccga tttgccatga actcggtaaa cgcgaacgca
    35101 ttcaactgat cagttccggc ggcacacttg agcgcaaata tggctgttac gtcaatccct
    35161 cgctgatttc ccaacttaaa tcgctggaaa tcgatctgtt tattttttct tgtgaaggga
    35221 tcgatagcag cggcgcactg tgggactcca atgccatcaa cgctgattac aaatcgatgc
    35281 tattaaaacg tgccgcgcaa tcgttgttat tgattgataa aagtaaattt aatcgttcag
    35341 gggaagcccg catcgggcat ctggatgagg taacgcacat tatttctgat gagcgccagg
    35401 ttgcaacttc tttggtaaca gcctgacgga aagggtagca ggccggagac gacgccccgg
    35461 ccttgcctgt tatcgctcgt cgcgacgacc acccaccgca gcccagatgc ggcggacgtg
    35521 caccgtcact tcttcgcgat cgtgatacaa ctgccgcgcc tgaatctgag catttatgcc
    35581 atgttcatca agctgtgcct gaatatacgc cagattgtgt gatacttctt cgtagcgttt
    35641 tttcatcggc agtttgagat tgaaaatggt ttcacggcac cagccattaa ccagccactg
    35701 cgccatcaat gccgcaactt tagccggttt ttcaaccata tcgcatacca tccaggagat
    35761 attgctgcgt gtcggacgga atttgaaacc gtcttcccgc agccacgtca cctgcccggt
    35821 atccatcaga ctttgcgcca tcgggccgtt gtcgacggaa taaacccaca tgttgcgctt
    35881 caccagttgg taggtccagc cgcctgggca agcgcccaaa tccaccgccc acatcccgtt
    35941 cgccaggcgt tcatcccact catccgcagg aataaacaca tgaaatgcct cttccagttt
    36001 gagtgtggaa cgactcggcg catctgccgg aaatttcagg cgcggaatgc ccatatagaa
    36061 cggcgaatta ttgttgctgt atgagtaacc ggtatagcag caacctggtg caatgaagaa
    36121 tacatgcaca accggacgct tcggcgtttc atagttcgcc agcaccccgg catcgcgcaa
    36181 ggcagcgcgt agcggcacgg tgaatttacg gcagaatttc agcaactctt tgctttcgtt
    36241 ggtatcggca acttcaacac gcagttcacc gcccttctct actacgccct gtaacatgcc
    36301 gacaatgggg gtaatacgat cttctggcgg caaatgctgc aggagttccc ccaccacaaa
    36361 ccactggcgg gcaaaaatta atgaactgaa cggcagctca cggattaact tatcgccatc
    36421 atcaggttga taacattcat aaatgacata acccgcattc tctttcacgc gggcaaaacc
    36481 gaaaatttcc cgctggccgg ctttatcggt aatttctgcg gcgcactctt tttcaaagcc
    36541 cggacggcac agcaatacaa ccttattcat gacttacgcc cttgcgcttt aaacggatag
    36601 caccaactaa cattaacgcc cagcccgcaa ggaaactcac gccgccaacc ggtgtgacaa
    36661 acgcccacaa acgcagatgg gacagtgcca ggcaataaag gctgccgctg aacagcaccg
    36721 tgcctaacgc gaggaaaacg ctactccagt aaaaccagat actgatgcga cgctgcattg
    36781 ccaccgccag acctaagatc gccagcgtat gaaacgcctg gtattcgagg ccggtctgga
    36841 tccagcccat ctcaacggcg cccatggttt tacttaacac atgcgcgcca aaagcgccca
    36901 gagccacaaa aatgaagccg ctaatggcgg cgaaaatcag cataaaacgg ctggtcatgg
    36961 tcgtacccta cgtaaattat tgttcataac gaaagcggaa tttttcttgt tcagcagcgg
    37021 ctttcgccag gatccattgg cgaaaggcgg ctattttacc cagttctgcc tggctgtcat
    37081 gacaaaccag ataaaaagcg tttttactga ccagaacatc attaaacggg caaacaagac
    37141 gtccggcttc aatttcagat tgcgccatca cgttatttgc cagcgccact ccctgcccgt
    37201 gaatagccgc ttgcagcacc atggcgctat ggctaaaaat tggcccttgc tgaacgttga
    37261 tatgatttaa ccccaattgt cgggtatatg tctgccagtc acggcgcgaa gcatcatgta
    37321 ataacgtatg tttagccaga tcttccgggg tcttcaaggg tttttcgcca gtcagcagta
    37381 gcggcgaaca caccggcaat aaatattcgg cgtaaagttt ttccacccgt agccccggcc
    37441 agttgccccg accataaaat atcgccacat caacatcatc cgccagctta tcttcctgac
    37501 gatcaaccgc ctggattcga acgtcaattc ccggataagc tgaattaaag ctggaaagtc
    37561 gcggaaccaa ccaatgaatg gcgaaactgg ggagtaaact gaccgtcaac gcccctttgg
    37621 cgctacgggc ctggagttta cgcgtcgctt cggttaattg cgaaaatatc tctttgatat
    37681 cgaggaaata gctttgacct tcctcggtca gcaggagtga acgattacgg cggcggaaca
    37741 gttttagccc cagaaaatcc tcaagagact tgatttgatg acttactgcg gcctgggtca
    37801 caaaaagctc ttctgctgcg cgagtgaaac ttaaatggcg tgctgcggca tcaaaaactc
    37861 gtaaggcatt tagcggtggt aatcgtttag acatggcttt taaactttga tgttaaatga
    37921 atttaacaat tagatcacac tgtgtaacct attagttttt ttaatctgag ccattataaa
    37981 ttgtccgttg agcttctacc agcaaatacc tatagtggcg gcacttcctg agccggaacg
    38041 aaaagtttta tcggaatgcg tgttctggtg aacttttggc ttacggttgt gatgttgtgt
    38101 tgttgtgttt gcaattggtc tgcgattcag accatggtag caaagctacc ttttttcact
    38161 tcctgtacat ttaccctgtc tgtccatagt gattaatgta gcaccgccta attgcggtgc
    38221 ttttttttac cttgcgatcg cgaattactg atccagttcg accatctctt tcacgtcggt
    38281 acggttgatc tgctgtttgt tgccattagc gtctttatac gaaatcatac cggtatcgtt
    38341 atcagtctgt ggtttgccgt cagagacgat ggtacgtccg tcattggtgt gcatcacata
    38401 gttcgaaccg gaacaggcgc tcagggcaaa agtcagcata caggcagaaa taattgcggc
    38461 agtctttttc atcatcttct ccttaaagcg attaataaat cttaatagta ttgattaaca
    38521 ggctaaaatt aacgcctgac actattcagc atatgttact gacgcggctt cgccaggata
    38581 tccagataat tctgatggtt agcactctcc ttgtatcaaa gtgaattttg cgtcacgatc
    38641 ggtgcatcaa gccgaggagt accatgaacg tttttaatcc cgcgcagttt cgcgcccagt
    38701 ttcccgcact acaggatgcg ggcgtctatc tcgacagcgc cgcgaccgcg cttaaacctg
    38761 aagccgtggt tgaagccacc cgacagtttt acagtctgag cgccggaaac gtccatcgca
    38821 gccagtttgc cgaagcccaa cgcctgaccg cgcgttatga agctgcgcgg gaaaaagtgg
    38881 cgcaattact gaatgcaccg gatgataaaa ctatcgtctg gacgcgcggc accactgaat
    38941 ccatcaacat ggtggcacaa tgctatgcgc gtccgcgtct gcaaccgggc gatgagatta
    39001 ttgtcagcgt ggcagaacac cacgctaacc tcgtcccctg gctgatggtc gcccaacaaa
    39061 ctggagccaa agtggtgaaa ttgccgctta atgcgcagcg actgccggat gtcgatttgt
    39121 tgccagaact gattactccc cgtagtcgga ttctggcgtt gggtcagatg tcgaacgtta
    39181 ctggcggttg cccggatctg gcgcgagcga ttacctttgc tcattcagcc gggatggtgg
    39241 tgatggttga tggtgctcag ggggcggtgc atttccccgc agatgttcag caactagata
    39301 tcgatttcta tgctttctct ggtcacaaac tgtatggccc gacgggtatc ggcgtgctgt
    39361 atggcaaatc agaactgctg gaagcgatgt cgccctggct gggtggcggc aaaatgattc
    39421 acgaagtgag ttttgacggc ttcacgactc aatctgcgcc gtggaaactg gaagctggaa
    39481 cgccaaatgt cgctggtgtc ataggattaa gcgcggcgct ggaatggctg gcagattacg
    39541 atatcaacca ggccgaaagc tggagccgta gcttagcaac gctggcggaa gatgcgctgg
    39601 cgaaacgtcc cggctttcgt tcattccgct gccaggattc cagcctgctg gcctttgatt
    39661 ttgctggcgt tcatcatagc gatatggtga cgctgctggc ggagtacggt attgccctgc
    39721 gggccgggca gcattgcgct cagccgctac tggcagaatt aggcgtaacc ggcacactgc
    39781 gcgcctcttt tgcgccatat aatacaaaga gtgatgtgga tgcgctggtg aatgccgttg
    39841 accgcgcgct ggaattattg gtggattaat gacaaacccg caattcgccg gacatccgtt
    39901 cggcacaacc gtaaccgcag aaacgttacg caataccttc gcaccgttgt cgcaatggga
    39961 agataaatat cgccagttga tcatgctggg gaaacagctt ccggctttgc cagacgagtt
    40021 aaaagcgcag gctaaagaga ttgccggatg cgaaaaccgc gtctggctgg gatataccgt
    40081 ggctgaaaac ggtaaaatgc acttctttgg cgacagcgaa ggacgtatag tgcgcggcct
    40141 gctggcggtg ctgttgactg ccgttgaggg gaaaaccgcc gccgaattgc aggcacagtc
    40201 gccgctggca ttgtttgatg agctgggatt gcgtgggcag cttagcgcct cacgcagcca
    40261 ggggttgaat gcgttaagcg aggcgattat cgctgcgacg aagcaggttt aagcacagaa
    40321 ccgggtcgga taagacgttc acgtctcatc cgacctgatt gtttaaccct gacgcgccgc
    40381 tttcgccatc atcttcttca gcgcatgaga aaccgcaaca aacccaaagg tggcggtcac
    40441 catcgttgcc gcgccaaatc cagatgcaca atccatccgc ttcggccctt ctgccgtggc
    40501 tttcatcgca cacaccgtac cgtctgactg cgggtacacc agcgcttcag tagaaaacac
    40561 gcaatccacg ccgagcttac ctttactgtt tttcactacg ccaaaatcgc ttttcaggcg
    40621 ctcgcgcaac ttcgccgcca gcgggtcctg aatcgttttc gccagatcgg taacctgaat
    40681 ctgcgtcggg tcaatctgcc cacccgcgcc accggtcgtg accagcggga ttttattgcg
    40741 ccgacaataa gcaatcagcg ccgctttggg tcgtacacta tcaatggcat caatcacgta
    40801 cgaataaccg gcactcatat actgcgctac gttatccggc gtcacgaaat catccaccac
    40861 cgttacacgg cactccgggt taatctggcg aatacgttcc gccatgactt tcgcttttgc
    40921 cagcccaacg ttatcgcgca gggcatgaat ttgccgattg gtattggtga cgcacacatc
    40981 atccatatcg ataagcgtga ttgcgccaat ccccgtgcgc gccagcgctt ccgccgccca
    41041 ggaaccgaca ccaccgatac cgaccacaca aatatgcgcg tcagcaaaca gttgcaacgc
    41101 tttttcacca tacagacgcg ctgtgccacc aaaacgctga cgccatgcat cactaattac
    41161 cacagacata gcacctcaga attaaaaagg gtgaggtttt ccctcaccct aaacgcataa
    41221 atgcctgatg cgctacgctt atcaggccta cataactcct gcaatatttt ggatttgcat
    41281 gatttgtggg ccggataagg cgttcacgcc gcatccggca tgaacaacgc gcacttagtc
    41341 aataatcagg atgtgaggtt acccctcacc ctgtcatatc cgtaaaaacg gcatacagaa
    41401 tatcacatca gccgctaaag acgttatctg cgcccggggc ggttttcagc acccagacac
    41461 gtccatagtg gttgtaccaa cctgcgcggt gtccggcttc cggcccgatc ccttgataga
    41521 tatcgaagtg ttggcctttg attgcgcccc cgacatccaa cgccaccatc agacgcaatt
    41581 cgtactgacc gttaaatttg ccgttattat ccagcagcgg cacttctgcc agcaaggtag
    41641 tacctggcgg aataattgaa cgatcggagg caacagaagc acggccaacc agcggcaccg
    41701 cacttgcccc tttcaccggt gcaaaagatt gcggtttaaa gaagacgaaa gacgggttct
    41761 gttccagcag ctcgcgaacc tcggcttcac tgtgtgtttc gccccaatga cgaatcgcct
    41821 gcatcgacat atcttctttt ttcacttcgc cacggtcgat cagcacctta ccaatgctgc
    41881 gataggcatg accgtttttc cctgcatagc tgaaaaagtt aagcggactg ccatcaccaa
    41941 agtcgatata cccgctaccc tgcacatcca taatgaagtt atccatcagg gagttgctgt
    42001 aagcgagaat atatttatca ctcagcgccc ctgcgtagat ctccgcacga gacggcagac
    42061 gaccacgttt tggcggcata cggtaaatcg gatactggaa ctcgccctgg cgggtatggc
    42121 gcgcctgaat taccggcgtg taatagcctg taaactgcac gttaccatag ttgtcggcac
    42181 cttccatctg ccaggcatca atgccgaact gacgcatatt gcgggtatca ccgcctgcgc
    42241 gcagccactc ttgcaccgcg ttataaacat tactctggtt gccatacaga cgcggtgacg
    42301 aattacggat atggttaatt tgctcggcaa aatcaccggc gttaatcggc gcgccaacgg
    42361 catctggctg gttcaccaga gagaaaggct gggtaaattt cccgtcttta tattgctgtc
    42421 cgcgatcggt tggtttggaa gagcaggcgg caagcattgc cacaaccgtg cccataagaa
    42481 ggtactttac ccaacgtcct ttcattgttc tcttcttcag gttaaaaaat aaggcgcaac
    42541 gaagataaca aaccgccgcg tttaatgaaa tgagcagcct ctccctgacg caaattttgc
    42601 acaaaaaata ggctttagtg atttgttttt gttcaaaatc atgccaaatc cgtgatcggg
    42661 gtaaaaaaaa ggttgcatga aaacgcgagc ggagtatagt gcgcatccac ggacgcgggg
    42721 tggagcagcc tggtagctcg tcgggctcat aacccgaagg tcgtcggttc aaatccggcc
    42781 cccgcaacca attaaaattt gatgaagtaa agcagtacgg tgacgcgggg tggagcagcc
    42841 tggtagctcg tcgggctcat aacccgaagg tcgtcggttc aaatccggcc cccgcaacca
    42901 atcaaatttg atgaagtaaa agcaatacgg tgacgcgggg tggagcagcc tggtagctcg
    42961 tcgggctcat aacccgaagg tcgtcggttc aaatccggcc cccgcaacca attattgaac
    43021 accctaacgg gtgttttttt gtttctgctc tcccataaaa aaagcgccat tcagcgcctt
    43081 tttatcatcc ccttctcgcc agcgtcgccc catcggcaaa atacgcttta attcccgcaa
    43141 gaatagactc cgcaacttcc tgctggaaag tcgccgtttt cagtttacgc tcttcctcaa
    43201 cgttactgat aaacgccgtt tcgaccagaa tggagggaat atcaggcgct ttcagcacgg
    43261 caaacccggc ctgttcaacc tgatttttat gcagcttgtt gattttaccg agcttattca
    43321 gcaccgcttt accaaactta aggctgtcgg caatggtcag cgactgcacc atatcgaaca
    43381 tggtgtggtc gacatagcgg tcaccgcttt tgctcacgcc accaatcaag tccgaggcgt
    43441 tctgggtctg tgccagatat tttgccgcag tactggttgc gcctttggtt gagagcgcaa
    43501 acactgagga accgctcggc tggcgactgg taaaggcgtc ggcatggata gagacaaaca
    43561 ggtcggcacg ctgtttctgg gcttttgcta cgcgcacttg caacggaatg aagatgtctt
    43621 cattgcgcgt catgtacacc ttcatattgc cctctttctc gatcagagag cgcagacggc
    43681 gagctatttg caataccacg tctttttcgc gcgttttgta tttccccacc gcaccggagt
    43741 cttcgccgcc gtggccaggg tcaagcataa taacaatagg acgatcgcgc cctgctttac
    43801 ccggttgtgg accactttgt gctggcggca cctgcttttc gaggtcgcct ttgttgtaat
    43861 cctccagcag cgccagcagc gggtcctgca tatcctgtgc attggccgga tagaggtcca
    43921 tcaccagacg ctctttaaac ccggcgaccg gcgcaagggc aaacagctgc ggttttacgt
    43981 tttgctttaa ttcaaaaacc atccgtacgg tctgcgggtc aaattgcccg acgcgcgccg
    44041 acttgatgaa cgggtcgtct gcacgaattt gtgccgccat ccccttgagt accgagttca
    44101 ggtttacatc ttcgatatcc accaccacgc gttccgggtt actcaacgcg aactgcttat
    44161 atttcagctg acgatttgat tctaccgtca cgcgggtgta gctggacgca ggccagacgc
    44221 gcaccgccac gacctggcta accgcagcca ggctgacctg acttacgctc aatagccaca
    44281 tagcgcccgc gccttgcagt aaacgacgac ggctgattgc agtgttggat cctgacatgc
    44341 ctctcccgag caaaagaaat ctaagctgtg taacaagtaa acgactaatt tgaccggaaa
    44401 ctttagcgaa agacgcataa tctgtcatct aataaacggt aaacattctt tttatattca
    44461 cggcattact gataaaaaag tcgctctcgc ataaaattta cacttgcacc ctgcgaaaaa
    44521 acagaataaa aatacaataa tttcgaataa taatgcaaag gggtgtgccg tggtaaagga
    44581 acgtaaaacc gagttggtcg agggattccg ccattcggtt ccctatatca atacccaccg
    44641 gggaaaaacg tttgtcatca tgctcggcgg tgaagccatt gagcatgaga atttctccag
    44701 tatcgttaat gatatcgggt tgttgcacag cctcggcatc cgtctggtgg tggtctatgg
    44761 cgcacgtccg cagatcgacg caaatctggc tgcgcatcac cacgaaccgc tgtatcacaa
    44821 gaatatacgt gtgaccgacg ccaaaacact ggaactggtg aagcaggctg cgggaacatt
    44881 gcaactggat attactgctc gcctgtcgat gagtctcaat aacacgccgc tacagggcgc
    44941 gcatatcaac gtcgttagtg gcaattttat tattgcccag ccgctgggcg tcgatgacgg
    45001 cgtggattac tgccatagcg ggcgtatccg gcggattgat gaagacgcga tccatcgtca
    45061 actggacagc ggtgcaatag tgctaatggg gccggtcgct gtttcagtca ctggcgagag
    45121 ctttaatctg acctcggaag agattgccac tcaactagcc atcaaactga aagctgaaaa
    45181 gatgattggt ttttgctctt cccagggcgt tactaatgac gacggtgata ttgtctcaga
    45241 acttttccct aacgaagcgc aagcgcgggt agaagcccag gaagagaaag gcgattacaa
    45301 ctccggtacg gtgcgctttt tgcgtggcgc agtgaaagcc tgccgcagcg gcgtgcgtcg
    45361 ctgtcattta atcagttatc aggaagatgg cgcgctgttg caagagttgt tctcacgcga
    45421 cggtatcggt acgcagattg tgatggaaag cgccgagcag attcgtcgcg caacaatcaa
    45481 cgatattggc ggtattctgg agttgattcg cccgctggag cagcaaggta ttctggtgcg
    45541 ccgttctcgc gagcagctgg agatggaaat cgacaaattc accattattc agcgcgataa
    45601 cacgactatt gcctgcgccg cgctctatcc gttcccggaa gagaagattg gggaaatggc
    45661 ctgtgtggca gttcacccgg attaccgcag ttcatcacgg ggcgaggttc tgctggaacg
    45721 cattaccgct caggcgaagc agagcggctt aagcaaattg tttgtgctga ccacgcgcag
    45781 tattcactgg ttccaggaac gtggatttac cccggtggat attgatttac tgcccgagag
    45841 caaaaagcag ttgtacaact accagcgtaa atccaaagtg ttgatggcgg atttagggta
    45901 agcgatgaaa attgtcggat gcgacatgcg taacactcgt gcgtcgcatc cggcaattac
    45961 gtttattccc gcgaactaaa caacgccgcc agaccactgc gccgctcagt acgagtggcg
    46021 attgccgcac ttaatatacg ctcatcggca tacagcgaca gacggcgacg cgcgcgggtc
    46081 accgcggtgt ataccagctc tcgcgttact accggcgtgc gttggctcgg taaaatcaac
    46141 gccgcatggt cgaactccga tccctgcgat ttatgtaccg tcatcgccca cgtagtttcg
    46201 tgctctggca agcgactcgg ttgcacagac ttaatattgc cgtccggcat cgcaaaccag
    46261 acgcgcgtcc cctgcccgcg atccagcgca ataccgatat cgccattaaa caacccaagc
    46321 gcgctgtcat tacgggcaat cattaccggt cgaccttcgt accaacgaga gtgcggatga
    46381 cgatgaattt tgcgcttctg ttgcataaac tgctcaattc gctcattcag tccagccacg
    46441 ccaaacggcc cttcccgcag ggcgcacaaa agttggtact cattgaacgc ctgaatgatt
    46501 aaatccggct cggcacgcgc ttgcagcaga tccagataac gtccataacc cgcaagagct
    46561 tcctcaagca tcgcaatata atcttctccg ctctgtaaaa gccgtttttc gatatcagta
    46621 aaatcctgct gaaaaacggt tttcactgcc gttttatcac cacggttgat cgccgcagct
    46681 aactgaccaa tgccagaatc actgccgaaa cgatagcttt tttgcagcag gcagagactg
    46741 tcgcgcaaag atgccgcttc tgtgccagtt cctgccggaa cgtgagttcc cgtcaggcgg
    46801 cttagctgcc cggcacgctc ggcggtaaag ccggcattgg cataagcgca gatatcgccc
    46861 agcacagccc cagcctcaac cgaggccagt tgatcacgat cgccgagaaa gatcactcgc
    46921 gcatgatcgg gcaaggcgtc gatcagtctc gacatcatag gcagatcgat cattgacgct
    46981 tcatctacca ccagcacatc aagatgcagc gggttaccgg catgatgacg taaacgctgg
    47041 ctacccggct gcgcacccag caatcggtgc aaagtactgg catcttccgg aatgcgtttc
    47101 ttttgttcat cggtcagcgg taactgtcgc aaagccttgc cgagagattc ggttaagcgc
    47161 gcggcagctt tacccgttgg tgcagccaga cggatacggc agcgttcgcc gtcggccatt
    47221 tgaattaacg ctgccagtaa cttcgccacg gtggtcgttt taccggtgcc tgggccgccg
    47281 gaaatcaccg agatccgccg cgtcagcgcc actgccgctg caactttttg ccagttaatt
    47341 tcatcgctta ctggaaaaag tttgtccagg gtttgcgcca gtagagcttc atcaacctca
    47401 atggcatgat tcacctcatt gaagaagcgc gctaccgtcc gctcgttaca ccacatgcga
    47461 ttcaaataaa gacgatcgcc acagaggatc atcggcgtgg gttcatctcc cctgctgacc
    47521 gcttgagaag ccagcaagca ttcttcccaa ttttgtagct caccgatttc actgacacag
    47581 gtcgccaaca gcggatgcga cgcctcgtta ttttccagtc gtgaaagcgg caaacagacg
    47641 tgtccctctc cggcatcatg acttaacagt gccgccgcga gggtgacggc aggatgttca
    47701 tctcccgcca cggtcagggc aaattgcaca tccagcgggc gtagctgttt gtgctccaca
    47761 gcttccagta attgcttttg caatttcatt acgcctcctc cagggtcata ccggcaaaca
    47821 tctcatccat cagggcaatc aacccggcgt tgggtcgggt tgtgtaaatc ccttgttgcg
    47881 gatgttcttt atcaacgcca cgcaggaaca gatagataac gccgccaaag tgacgatcat
    47941 agtcgtaatc agcaatgcga tggcgcagat aacgatgcag cgccagggta taaagctgat
    48001 attgcagatc atagcggtgt gcctgcatcg ctgctgccat agcctgttgg gtgtaagccg
    48061 aactgtcttc acccagccag ttggatttat agtcgagcag gtaataacgc ccttcgtggc
    48121 ggaataccag gtcgataaag ccttttaaca tgccgcgtac ctgcatgaac tccagcggcg
    48181 ggcagccagc ggatagcggg tcaaactggc gaattagtgc atcaagctgg ctggcgatga
    48241 gcggttcgct aatcggcagg taaaactcca tctccacctg tttatcgcgg tcggaaagct
    48301 gactcaggct aacacccgtt tcattgagag gtgcctggag gacagccgtg atccactcgg
    48361 tcaacaccgg ttcccactgc gattcaaagc cgccgagttc cagtttttcc tgtacccagt
    48421 tcgggtcaac cggctgggta aaatcgaggt cttcaaacaa actgtgcaag aatgtccccg
    48481 gtgacgcacc gcgcgggaac tgatgcggtg ttaacgtcgg ttcttcaacg acgctgacca
    48541 cgcctgcggc atcaacatcc agccgtggca tcagatcctg agcgataccg tgaccacgct
    48601 gctgcaagcc ggagtagctg gtgacacgcc agttatcgcc gggcaatcgt tgtagtgtcc
    48661 tcgcattcag ttctgcagtt aaagcatcat taacctgcca gggctggtta tcaccagttt
    48721 gtgccgtttg ccaggcaata tcatcatcgc ataacgcttc aatacaggtg cgaagccctg
    48781 ccgcatcttg cggttcccct ttttgcagca aacgcccgag cgcactttgg tggacgtcgg
    48841 tgtcaccttt tttatcgcca cgacggcgca ccagcggtgc aacgccgaga ctgcaatgcc
    48901 aaaccgaacg cgtcagcgcc acgtaaagca aacgcagatc ttccgccaga cgttcgacct
    48961 ccgcgaggtc gacgctttct ggcgcagcgt taagatccaa aactgcctca aacgagtggc
    49021 gatcgtgata aaacgcctga tcctggacgc ggaaattggt gataaacggc agccagacca
    49081 atggatattc cagccctttc gatttgtgga tcgtgacaat ctgcaccaga tgtttatcac
    49141 tttcgagacg catttgttgg ctggaggcat tactgtctgg ctcgaggata tgttgcgata
    49201 accagcgtac cagcgcatgt tcactttcca gctgcgttcc ggcttcttgt agcagttcgc
    49261 tgatatgcaa gatatcggta agacgccgct caccgcctgc cgttgccagc aagttttcag
    49321 caatgttacg cgccgacatc agcgcccgca gcatcggcat aacgccacgt ttgcgccaga
    49381 tttgccgata accatcgaac tcttcgacta ccacatccca cgcatgttcg tcattgttca
    49441 gcgtttcgat atccagcgcg ttcagcccca tcattgacgt tgccagcgca ctacgcaggg
    49501 tgttctcacg ttcgggcgtc atcaccgcct gcaacaacca aagcatttcc tgcgcttcca
    49561 gagtttcaaa aacactgtcg cggttcgaaa ggtaaacgga agggatttcc agcaacgtta
    49621 aggcgtcgcg cacctgggcg gcctcctggc ggctgcgcac cagcacactg atgtccgaag
    49681 cacgcaccgg acgcgcgtcg tcgccgttca tcagcaacgc ttcgccccgc tgtccggctt
    49741 gtagccagtc gcggatttgc gcagcacata cctgcgccat ggtactttga tagtcgccaa
    49801 cgccgcagct ttcgccttcc atcagccaca ttttcatcgc aggctgcgtt tcacctttaa
    49861 acacaaaacg taacgcctga tttttcccgg ctgatttcac tggaataaac ggtatttcgc
    49921 gaaacatgaa cgcgtcatca gtctggctga aaagcttgtt cacactgttc accattcctg
    49981 gtgcggaacg ccagttggtg tctaaagtgt agtgggcgtg aacttcgcta cgcgccttca
    50041 tataagtgaa gatatccgca ccccggaatg catatatggc ctgtttcggg tcgccaatca
    50101 gcaacaatgc ggtttccggc tgatggtgcc agatacggcg aaaaattcgg tactgctggg
    50161 ggtcggtatc ctgaaattca tcaatcattg ccaccgggaa tcgcgtacgg atcgccgctg
    50221 ccaacacctc gccgctttcg ctacgcagcg cggaatcgag ccgacttaac atgtcatcaa
    50281 aacccaattc gccacggcgg cgtttttcac gcgctactgt ttcgcggatc tcagccaatg
    50341 cgcgggttat caccagatca cggatcgaca atggttctgc aagcagttgt tcgatcgcct
    50401 caaacagtgg atgtcgcggg gtttcccccc cggctttcgt gcgatcttct aagaaacgct
    50461 gagagaattt ttccagcgac tccggtaact gataactgtt ggtctcttct tctgcccagg
    50521 cgctgatctt ctcgatccat ttagcctgat tgctacggtt aaacttgcgt cgatcaatac
    50581 cagaagattc gatcagcgca tccagttcac ccactgcgtc gcgccactgc tgttttaccg
    50641 tatcaatacg cgccacaatt tgcgcgtggc gggaagccag cgtttcatca tcgggcggcg
    50701 gtgctttgat aaccggtgct tcgccttgca gataacgatt aatatcgcgc agcaacgcct
    50761 gcggcccttt ccaggtttca aagaccacca gggcaatttc acgcggcagc gggtagcagt
    50821 ggcgacgcca gaaatcggcg caggcctggt agcgtagcag agactcatct tcaatcagct
    50881 gctgctcaaa cagcataccg gattcaaagg cattcaggtt gagcatgcgc tggcaaaaac
    50941 cgtgaatagt aaagactgcc gcttcatcca tctgccgttc ggctaacaac aaccactgcg
    51001 cggcttgcgc tttatcgtcg atctcttcca gcaggcgttc gtacagtgga ttgtcggtgg
    51061 tttcacgcag acaggcgatg cgcaactcgt ggatattgct acggatacga ccgcgcaatt
    51121 ctgccgtggc agcctcggta aaggtgacca ccagcagttc ttcaacggtc agcgggcggg
    51181 gaaaggcggc ggaaccgcct agtccaagta acaggcgcaa atagagcgcc gcaatcgtaa
    51241 aggttttgcc tgtgccggca gaggcttcaa tcaggcgctc gccctgtaag ggcaagcgca
    51301 aaggatctag tgtctcggcg acatcactca ttcttttcac tcatcagggg cattgtttgc
    51361 tgcaacgcgc tgacgttctc ccacactttc cagccttcag gatgcacata ttcggctttc
    51421 ccgttctggc tgccggaaat ctgcgacagg atagccatgc cttgcggctc gaccaccgcc
    51481 tgatggaaga aatcagcaag tttttgcggc gtcagcagtt ttatctgggc cacgatttta
    51541 tcacgcgaat cgaagcgcat attgccgcga tcgaaatctt tacttaactt cgatgcttct
    51601 tcgccgagcg tttgcggtgc ctgcagcatc tgggtaatta ccgcctgctg gatttgcgca
    51661 aactcatctg gcttcatcgc tcgcaatttt gcctctgcgg ttgggaaaaa cgccttgtaa
    51721 cgctcccaca agaatgaagg ctgtttatca ttgctttgca aaaggaagcc catgccccac
    51781 tgacgcccca cgctcattgg aaacgcaaac acggcatagc ccaattgttc ttcggtacgc
    51841 aactgattgt agaaccacgg ctgtacgatc tgccccaaca gagagctata ggctgagctg
    51901 gtgtattcat cgtagccagt cggtacaaat accgctgcca gtgcggagtc ggtgctgtta
    51961 ccggcttttt caaagatgac ggattgtttt ttatcgacca ctacatcttt gtttcgacac
    52021 cactctgaac catcagcgcc caactgtttt tgcacatcgc gtgccagcgt tgttgcctgg
    52081 gcctcggtca tgttgccgat aaccataaac tctggtcgag cccctgattt taaggcgtcg
    52141 cgataggcca gcacctcttt caacgtaatg gagggcaaaa ttttacgccg ttcatctcgc
    52201 gagaagtacg gcacttgcga gagcatctgc gcgggcataa tcgcctgctc aaacgcttta
    52261 cccttttctg cggaatccat catctggtta taccaggact tcgcctgctc aagctgatct
    52321 tccgtagcgg tatagctaaa gtacccctcg agcaatgcct ggaacagctg cggcagacgc
    52381 tgggtgtaac cattagcatt aaccataagg ccgttgttag cgttggtgga aaaacttatg
    52441 ccaccaaccg acgcctggtt gcttaactga tcaagcgcca gccctgcgag ataatcattg
    52501 agcgcaaaca tcacctgatt gcgggcgctg tccatggctt tcggattacg caaaatcagg
    52561 ctgacatcag ctttgggttc gctggaaaaa taacggcttg gcgcatacac cacgcgcaga
    52621 ttcgactcat caacaatcag ctctggatgg tcatatttct tctctgactt aatcagcgag
    52681 aaatcgtcag gaatataggg gttaagctcc ggtaaggaga gcgcaatatt ggcagctttt
    52741 tgctgccagt cagcgaaagt ttgttcgctg attttatcga cctgatacgg cgcatcgaca
    52801 aaataagccg ttttattgtg cggctctttc gggctgatat accagatacg cgcattctgc
    52861 ggcgtcatca tcgccagacg ttcttttact gctttagcat cgtaccgatc ggcaatattg
    52921 actgcatcca gcgtatgctc aaccggaacg cgaatcatgg tatctgccag ccattcgacg
    52981 taatccatat cacgggtgat cgacggataa cggaagtcga tatccagcac attcgccagt
    53041 tcatcgaagt attgtttatc aatgcctttt tcacgtaaca gattgagata gctaaaaatt
    53101 gccgccacaa cctgatcgcg attagccagg cctttatcgg ttaaagacgc agagatcgct
    53161 aatacgccgc tgttgccgtt gacgatagga tcggagttgg cgctaatgcc ctcaactaat
    53221 ccctgctttt gcagccagtc ggaaagtgta cctgggctgc gattaccaat cagataggta
    53281 atcaattcat ccgttttact acggaacttc gctgagttgt tatcgatgcg aaactcaacg
    53341 cgcagaactt tacgcggcag tgccgggacg taatgaatga taatgccctt ttgcgcgtcg
    53401 gtgactaccg gcacggtgat ttccggtttt ttgctctctt tgttcggcac gcgaccaaag
    53461 gtgtccgccg ccatttttgc caactccggc agcggtttat tgctgtaaat aaccgccttc
    53521 atcagattgg cggaatagta cttctcgtgg aaatctttca gcgcctgctg caccggatta
    53581 ccaggtttat cgcttaaagt ttcgaggtta ccaccagaaa actttgaacc ggggtgtgcc
    53641 gggttaatgg tttctgcgct gacctgtgcc atgcgcatcc cgtcacgcgt acgcgccatg
    53701 gttaattcag cgttcaccgc attacgctca cgttcggcat atttcttgtc gagcaaaggt
    53761 tcagcaatag catcggccag gcggtctacc gcaccaggca aggcgtcgtt ctcaacttcc
    53821 agatagaaag ccgtgcgata cggcgcagtg ctggcattgt gactaccgcc gtgcattttg
    53881 agatattcgg ccagactgtc agcctgcggg tactttttcg accccatcag actcatatgt
    53941 tcaaggtaat gtgccagccc ctggtacgcc tcgggatctt ccagcgaccc aacgggcacc
    54001 accagcgccg agagcgattt aactgcctgc ggatcagaaa ccagcaagac caccataccg
    54061 ttatccagac gtatagcctg atactggcgg ttatctttat cacttttacg gatggtttcc
    54121 tgaatcggct gccatcccgt ttctgcctga cttaagggtg cccaaagggc aacaaacaac
    54181 aataatgctt tgaaccaggt gctgcggggc attcacggac ctcataatca actttatttt
    54241 ctggccagat tcaacaggtt aacgcaaatc atctgccaga atttaatctt gtgctgcacg
    54301 agtcagccta tgtctatata tccatcagtc cgtgactggt gcgcatcata atgtaagcga
    54361 atgaattgcg caatttttat acagcactca tgactgatta aagcgaaaca gcggtaacag
    54421 gaaacgttgc gactgttcaa cgatggcctc cattgtctct ggtgttaatt gccgccagag
    54481 ccgttgatac cagatatcat caccttcgcc acgcaccatc atgttgcctt cgtaagcctg
    54541 aaggaatttc gtacgggctt tttgcaacgt ggaatcgtca tccagcatgg catcgttttg
    54601 cgcgtcataa caggttttta gccacgcgcc gccactttca ggtaacacca gcaatggcgc
    54661 ggacattcct tcacgatacc cctcaatcag ttgtgagagg taatgcaaag cctgttcggc
    54721 tgcaagcggc ggaaaacgcc actcgccgtc tttgcgtaga aaaaggcgac tttcaccatt
    54781 accaccgctg gcacagtaga caaggtgttc cagccaaagt tgcattccct gggccacact
    54841 taataaagag ggacgccagc gcaacaggcc atccggctgc acctgcggca accagccagt
    54901 tatctgcaca ccgttgcagg cgagatcaat ttccatactc tgccccggct ggcgacaggc
    54961 aatgactctg tcggcaagct gctgcatctc ctggcactgt gtttcccaga aaatttcacc
    55021 aaaagcgcca tacggtaaat cccctgccgc ccggaagcgg cggaacaagc gttcggcatc
    55081 atcctgctca accagtgcat tcaataactg ctgattgatt tgataacggc taagcccttc
    55141 cagaataaat ggctcggtgt cggggatttc gctgtcttcg gtacggaagt tcacctgcaa
    55201 acgcatctgg aaaaatgccc gcaccggatg tgcccagaat cgttgtagcg tttccagcgg
    55261 cacggtttcc ggtaaggtaa acggcagcgg ctgaacaaat tcagaatgtg ctttaccagc
    55321 ctggctggcc gcaggtagcc attcacgagc ataactttgt cgttcgcctg gctggtagtt
    55381 ttgcggatca aacggcattc gggtatgaag gcaagtaaga tgcgatttta cccttgcctc
    55441 gctttcatca cagttgagcg cctcatcgcc cggtagataa tgactttgtc cgatgtagtc
    55501 gatcagttcc tgcaccagta ccgacgggaa acgttcactg ttatcctgaa tggaacgccc
    55561 gatatagctg atatagagtt tttgctgcgc ggaaattaac gcttccagga acagatagcg
    55621 gtcgtcatcg cgacggctac ggtcgccacg cttcggtttc tggctcatca ggtcaaagcc
    55681 caatggcgca agctggcgtg gataaacgcc gtcgttcatt cccagcaggc aaaccacttt
    55741 gaatgggatg gaacgcattg gcatcagagt acaaatgtta accggtccgg cgagaaaacg
    55801 ctgactgata cgttcctgat ccaggcgctg tgccagttca tcacgcaata gtgacagcgg
    55861 caccgcgtcg ccatactgcg cacctaaacc ttcggcgata atcgcctgcc attgttgttc
    55921 gatcagcgtc atcgccgctt cggtttccgc atccggcagg aagaaggcgt tgagcatatc
    55981 gcgacaaacc ggcaaccact cttccagcgg acgctcctgc gccagcccgc gacgccagat
    56041 gtttagctgc attagcagtg aagccagatg ccccaccagt tctgcaatta agccgctcga
    56101 ttcatcataa ggtagaaccg attgccactc gccctgcgcg ctctccatcg cgtagcccag
    56161 caacatacgc gtcaggccaa accgccaggt gtgttgaccg gtggcgggga gttccagctc
    56221 gcgaacgtta tcgtcatcta tcccccagcg aatgccggat tcgttgaccc actggcgtaa
    56281 ataacgcagc ccttcttcgg tgatgtcaaa ccgcgccgcc agcaccggca catccagcag
    56341 cgccagcaca tcctctgaca caaagcgact gtcaggcagt gataacaggc tgataaacgc
    56401 ttccagtacc ggatgtgact gccgcgcccg acggtcggaa atggcgtaag gcaggtaacg
    56461 atccgcaggt gcgctaccaa acacagcctg aataaacgga ctgtagctgt cgatgtcagc
    56521 caccatcacg atgatgtcgc gcggagtaag tgtcgggtct tcctccagca tcgccagcaa
    56581 gcgatcgtgt aaaacttcaa cttcacgctg cgggctatgg caaacgtgga aggtgatact
    56641 gctatccagt ggatcaagcg ggcgtttgtt atcgctacgg gaaaactctt cgatgttcac
    56701 acccgcaacg gcgcggtttt ccagttccag aatgtcagac tgaatgttat gcagcaggtt
    56761 gtctggcgtc acatcgacaa aagcgtccag ctcctggctg ctctccaggt cagaaaggag
    56821 ataaatgtag tcgcgcccaa gcttccccca tgaggccagc agcgggttgc cgacatcctg
    56881 ttcaccatcg ctgttaaaga gctgtccggc attttcgcta tcgcgaaata gcggcaattc
    56941 gcgatcttca aaactgtgtc ggcgctggcg agtcagtagt ttcgccagat aagcaggatc
    57001 tttaatgtcg ccccagtaat aacggcaggg gttggtaaac aggagatgga tttcaatatg
    57061 tttacccagc gcctgtagcg cctggagata aacaggcggt aacgcggaaa taccgcatat
    57121 aaagacgcgc gaaggtagcc ccggcgggca ggtcgtcgcg gactccagcg tttcgataaa
    57181 gcgctgatag agattggcgc ggtgccagcg cggttgcccg agttgatggg tatattccac
    57241 caacgccttc cacaacggcg cttgccaggc ctgtgcttct cccagccctt caaccaaatg
    57301 tcctgtttcc cactgtgcca gccagtccgg acgatagacc agatactggt caaacaggtc
    57361 cgccgctttt gaagaaagct ggaacagttt tcgcttgtcg ctatcgtcag tcagataatg
    57421 ccgcaacagg gtaaagtctt cgcgctccaa caattgcggc agcagagtca tcagtttcca
    57481 gctcatgctc tgtttgttaa aggcgctctc tttggggatt tccggtaaca cccggacgaa
    57541 catatcccag ataaagctcg ctggcagcgg aaaatcaatg tttgccgcaa taccaaactt
    57601 ttgcgacagg gtcatttgca gccactgcgc cataccggta ctttgcacca gaatcatctc
    57661 tggttcgaaa ggatcgtcca gccgttcgcg ttcgacaata aactccatca acgcttccag
    57721 cacgtccaga cgattggaat ggtagaccct taacatagcg gctcctgact actgacgatt
    57781 cgggcaatgc aggcgggtca tctcgccttc tctgcccccg ggtgaaacta gcgtaacgct
    57841 gatgctgaca catcccgcct gcgatgtctg cattcggttg acctgccagt tggcaggtgg
    57901 cgaaatcgcg cgcagttgcg tttgctgcca accatgccgc cagagctgtt ggtactggtt
    57961 tctgctggcg agactgttca ttaatgttcg ctggataccc gataacgcag tgacaatcat
    58021 caccatcagc accatcgcaa ccattacctc cggcaggcta aagccttgtt gattcttcag
    58081 ggaagctgac ataacgccct ctctttcagc ggacaaaaat cgctccagcc gcgtggcgaa
    58141 aaaacaatgt ttccatcaat gacttcgcct gttcgccaca acgaaacgcc ttcataaccg
    58201 gcaatcaata aggcttcatt atctgccagt aaacgcaaac aaacctgggc atcggttcca
    58261 gcgtactgcg agcactgaac tgctggctgc gtctgccagg agtgcatttt tccccacgcc
    58321 agcgccgact gaacgatggc ctggcggcgc aatgactgac tttccatgct cacgcgagag
    58381 gcaaaactgc gatcctgctg actcattcct tgcaatagca agctacccaa aaccagcagc
    58441 atcaggacca gagccagtga cgaaacacct ttttcgcggt tcacaggttg aatcctgtca
    58501 cgctatagct ggcatccacc acggtttgcg gttcagactt actggcagca cgcatattaa
    58561 ccgtcaacac cggcgagaag ccgctgacat cctgacgtac gacctgaaaa gtgtcgataa
    58621 tgatggcatc cggattagtg actttatccc agcccttacc ttcacaggat gtcgcaccgc
    58681 gtagcgtttc cagcacatgc tccttcagac gaaatccaat ctggtcagac tcttttaccg
    58741 gttcgcgatc ccagataccg ttactgttcg catcccactg cacaatgata cagtcaccct
    58801 gtccgacaat ttccagccct tcgccggtac agctgccatg acaataaccc gccctctgga
    58861 gatgcttcgc gacggtaaat acccgcagcc agatttcatc ttccagcgcc agcttacggg
    58921 tgctcgttaa actttcacgc tgtaacgcag gcagaaagcg tgcagccccc agcaacaata
    58981 cgctactgat cgccatagca atcaacactt ccagcagaga aaaaccttgc tcttttacag
    59041 gcatccttct gtttctcctt gctgacaaag ccggagtctt ccccacggcg aaaccaccag
    59101 ccaccactcg cccgttgagt ttttgaagcg aatatgcccg gcccatgcgg tattgcgcag
    59161 gccaaagaaa gcaagcgaag gtgtcaggtc gctcatttcg acttcgggcc agcgtggcac
    59221 aaagaccaat ggtgaactgc catgacaggt attggcccca gcagcggaac tcacaaggca
    59281 ccataacgtc ccctccctga taacgctgat actgtggtcg cggttatgcc agttggcatc
    59341 ttcacgtaaa tagagcaaat agtcccgcgc ctggctggcg gtttgccata gccgttgcga
    59401 ctgctgccag tattgccagc catagagtcc acttgcgctt agcatgacca aaatcagcat
    59461 cgcgaccagc gtttcaatca gcgtataacc acgttgtgtt ttcatgccgg cagtatggag
    59521 cgaggagaaa aaaagacgag ggccagtttc tatttcttcg gcgcatcttc cggactattt
    59581 acgccgttgc aggacgttgc aaaatttcgg gaaggcgtct cgaagaattt aacggagggt
    59641 aaaaaaaacc gacgcacact ggcgtcggct ctggcaggat gtttcgtaat tagatagcca
    59701 ccggcgcttt aatgcccgga tgtggatcgt agccttcaat ctcaaagtct tcgaaacggt
    59761 agtcgaagat ggattcgggt ttacgtttga taatcaactt cggcagcgga cgcggttcgc
    59821 ggcttaattg cagatgagtt tgatccatat gattgctgta caggtgcgtg tcgccccccg
    59881 tccagacaaa atcgcccact tccagatcac actgctgcgc catcatatgc accagtaacg
    59941 cgtagctggc aatgttaaac ggcaggccga ggaaaacgtc acaggagcgc tgataaagct
    60001 ggcaagagag tttgccgtct gccacataga actggaagaa cgcatggcac ggtgccagcg
    60061 ccattttatc cagttcgcct acgttccacg ctgaaacaat aatgcggcgc gaatccgggt
    60121 cgtttttcag ctggttcagt accgtagtga tctggtcaat atgacgacca tccggcgttg
    60181 gccaggcgcg ccactgttta ccatacactg gcccgaggtc gccgttttca tcggcccatt
    60241 cgtcccagat ggtgacattg ttttcgtgta gataagcaat gttagtgtcg ccctgcagaa
    60301 accacagcag ttcatggatg atggaacgca ggtggcaacg tttagttgtc accagcggga
    60361 atccatcctg caggttaaaa cgcatctgat gaccaaaaat ggaaagcgtt ccggttccgg
    60421 tacggtcgtt tttctgtgtg ccttcgtcga gcactttttg catcagttct aaatactgtt
    60481 tcatggttcc tcaggaaacg tgttgctgtg ggctgcgacg atatgcccag accatcatga
    60541 tcacacccgc gacaatcatc gggatggaaa gaatttgtcc catgctgatg tactgcaccc
    60601 aggcaccagt aaactgcgcg tcgggctggc ggaaaaactc aacaatgatg cgaaacgcgc
    60661 cgtaaccaat caggaacaaa cctgagacag ctcccattgg gcgcggttta cgaatataca
    60721 ggttgaggat aataaacagc actacacctt ccagcaacag ctcgtaaagc tgtgatgggt
    60781 ggcgcggcag cacaccgtaa gtatcgaaaa tggattgcca ctgcgggttg gtttgcagca
    60841 gcaaaatatc ttctgtacgg gagccaggga acagcatggc aaacgggaag ttcgggtcaa
    60901 cgcggcccca caattcaccg ttaataaagt tgcccagacg cccggcacca agaccaaacg
    60961 gaatgagtgg tgcgataaaa tcagagacct ggaagaagga acgtttagta cggcgggcga
    61021 agataatcat caccacgata acgccaatca ggccgccgtg gaaagacatg ccgccgtccc
    61081 agacacggaa cagatacagc ggatcggcca taaactgcgg gaaattgtag aacagaacat
    61141 aaccaatacg tccaccgagg aagacgccga ggaagcccgc atagagtaag ttttcaactt
    61201 catttttggt ccagccactg cccggacgat tcgcccgtcg tgttgccagc cacattgcaa
    61261 aaatgaaacc caccagatac atcaggccgt accagtgaag cgccacgggt cctattgaga
    61321 aaatgaccgg atcaaactcc ggaaaatgca gatagctact ggtcatctgt caccacaagt
    61381 tcttgttatt tcgctgaaag agaacagcga ttgaaatgcg cgccgcaggt ttcaggcgct
    61441 ccaaaggtgc gaataatagc acaaggggac ctggctggtt gccggatacc gttaaaagat
    61501 atgtatatga tccgcgctat aaccctccgc gaatcagccc gcccatgccg cgacgctcca
    61561 taaaggctgc aacctgatgg cgaacttcgg tcgccagttg cgcttccaga ctacgctgcg
    61621 caagattttc tgcgtcggca taatcaatgc gccgcagcag gtattttacc cgcgctacag
    61681 aacgtccgtt catagacaga tggcgatacc caagcccaat gaggattgcc acgcacatgg
    61741 gatcgcccgc catttcaccg cacaaacgga gatcgattcc atgtatttcc gcttcccggg
    61801 cgatcatcgc cagagctcgt aacattgcag gatgaagact gtcataaatg ttcgccaccc
    61861 gggtattgtt gcgatcaacg gccagaatgt attgagtcag atcgttggtg ccaacagaga
    61921 tgaaatcgac ccgctttgcc agatgcggca gcataaatac cattgacggc acttccagca
    61981 tgatgccgat acgtggtttg ggaatttcgt aaccgatcat ctcctcgact tcacgtccgg
    62041 cacgttcaat caggcggcgt gcttcatcaa cttcatcgag gcttgtgacc atcggcaaca
    62101 gaatattcag gttgcccgta gcggcattag cacgcagcat cgcccgcacc tggatcaaga
    62161 agatctccgg ctgatcgagc gtaatgcgaa tcccacgcca acccaggcat ggattctctt
    62221 cgctgatcgg catgtaaggc agctgcttat ctgctccgac atccagcgta cgcaaggtga
    62281 cgggtttatc attgaacatt tgcagcatcc cctgatactg cgccacctgt tcttcttccg
    62341 acgggaaacc actttgcagc atgaatggga tttcagtgcg ataaagaccg atgccatcaa
    62401 tacggctgcc cagtttttct tcatgttccg ggcttaaacc agcattgagc atgactttta
    62461 tacgctcacc gctttttaac tgcgcgggta aattgacgtc atcttccgcc agacggctaa
    62521 gctcaatctc ttcactaatt agccgctgat attcttgcag cagtaccggc tccggatcga
    62581 ccagcaattc accgcgatag ccatcaacga tcagcgtcct acgatgcagc accgaaggct
    62641 gaatatccgc gcccatcacg gtagggatcc ccagcgcacg taccatgatc gcagcatggg
    62701 agttggctgc gccatctcgc acgacaacac cgactaagcg atcctggggc agctcagcaa
    62761 gagttgtcgc tgacagttca tctgccacca gaatgaaacg ttccggccag gcgttcggcc
    62821 cttgattagc gtcatcaaga tgaaacagca atcgctgacc cagcgcacgt aaatcgccag
    62881 cccgctcttt gagatagtta tcgcttagcg cggcaaactg ttcggcaaat ttttcaatga
    62941 ccgtttttac cgcccactct gccaccgagc ctttatcaac ctcggcaaac aattcgcgac
    63001 gcagccgggt gtccgaaagt aggtgcgagt aaagatcgaa aatagccgcc gtttcttttt
    63061 gtgctccggc ggcaaagcgt ttgctgtagc ggcgaaactc gtttgccgct tcttccagcg
    63121 ccccggtcag tcgttcgcgt tccagagccg gatccagcgt tgatgcctga tacacctgtt
    63181 ccattaaagg taacgtggca tcctgccagc cttcggcaat cgccacacca ggtgctgccg
    63241 gtaatgcgcg gattcgcgtc tggcgatatt gcccaaacag ggcagtcaac tgcgactgag
    63301 aaagaatagc tgccatctgg gtggcaagcg tcaccaggaa ggattcttca ctttcgtcat
    63361 actggcgcaa ctctcgttgc tgtaccacca gtacaccaag caactggcga cgttgaataa
    63421 ttggtacgcc taaaaacgcg cggaaacgtt cttcttttac ggaggggatg tatttgaagc
    63481 tggggtgctt ttgcgcatct gcaaggttta tcggttccgc cagcctgcca accaggccga
    63541 cgatcccttc atcaaacgcg agcgttacag tgcgaccgcg tggttttttc agcccccggg
    63601 tcgccatcag gtagtaacaa cgtcgatcat gatcggccag gtagaccgaa cagacctcgg
    63661 tatccatcgc aagacagatg tcggtaacca gaatatttaa cgcctcattc aggcgtggtg
    63721 cgctggctac cttttcgact atttcgcgca ggcgagtgag cataattggc gtgacttaac
    63781 ctcttttacg tcgataagca gatgcgtttt gtggttttgg cgtattttcc tgcagtgaca
    63841 tcaccacact cgcgaactct ttcattaccc tacggtagac atcacgttta aatgacacca
    63901 cctgtctgac cggataccag taacttaccc atcgccagcc gtcaaactct ggtgtactgc
    63961 tggtttgcat attgatttct gcatcgccgc tcaccagctg caagagaaac catttttgtt
    64021 tttggccgat acaaaccggc ttcgtgtccc aacgcaccaa acgtttcggt aatttgtagc
    64081 gcaaccagtt acgcgttgaa gcaaggattc gaacgtcttt gcggcttaat cctacttctt
    64141 caaacaattc acggtacatc gcctgctctg cggattctcc ggggttgatt ccgccttgcg
    64201 gaaattgcca ggagtgctga ccaaatcgcc gggcccacat tacctgcccc tggcgattac
    64261 aaatcacgat acctacgttt gggcggtagc catcgtcatc aatcaccgga ctacctcaaa
    64321 ataaagcttt atatacgaat gattgtttca tactccagga agacggtaaa ccactctctg
    64381 cagggcatta cacactaata acaattgaat aactcacagt tatgtgcaga gttataaaca
    64441 gaggaagggg tggatagccg tttttattca ccttttctgt ggatagagtt gtgaagaact
    64501 acggaattac tacgggaaaa cccggagcat tctgaataag cccgatatag acattttaaa
    64561 atattcatta ttagtcaatg cattacatgt aatttgagta atgaaaaaga gtaattcgtg
    64621 acccaggtca cacctctcat ttacgggttg gcgaaagatc aaccaatgcc gtatttatcc
    64681 acagaatgtg ccactaagtt aagcactgaa ccactaaaaa ctggagtttc gtcgcacgtc
    64741 aaggctgtaa atggaaacag tagtggaggt ttttcacagt tatcccagct ttctgtggat
    64801 aacatggtgt aagatcctgt ttattttcag tgaccagatt tggaaaacct attgcagtgt
    64861 tgcgcaactc gattaccggc agcctaaaaa gcaatataaa tcagagaatt gaacaacaca
    64921 tgtggaaaaa gttacactgc gaatattcgg cacataattg ctgtttgttt tttaaccaag
    64981 gtatcatgac atgtcccaac ctcgcccact gctctctcct cccgaaactg aagaacagtt
    65041 gttagcgcaa gcacagcaac tttctggtta tacattggga gaactggcgg cacttgccgg
    65101 gctggttacg ccagagaatt taaaacgcga taagggctgg attggcgtgt tactggagat
    65161 ctggctaggt gccagcgcag ggagtaaacc tgagcaagat tttgctgctc tgggcgtgga
    65221 acttaaaact atccctgtgg atagtcttgg tcgtccgctg gaaacaacat tcgtttgtgt
    65281 tgccccgtta acgggtaata gcggggtgac ctgggaaacc agccatgtgc gccacaagct
    65341 caaacgcgta ctgtggatac cggttgaagg cgagcgcagc atcccgctgg cgcagcgtcg
    65401 cgtaggatca ccgttgctgt ggagcccgaa tgaagaggaa gaccgacagc tacgcgaaga
    65461 ctgggaagaa ttaatggata tgattgttct cggtcaggtt gagcggatca ccgctcgtca
    65521 cggggaatat ttacagataa gaccgaaagc agcgaatgcg aaagcgctta ccgaagccat
    65581 tggtgcccgg ggcgaacgga ttctgacgct gccacgcggc ttttatttga agaagaattt
    65641 caccagtgca ctactggccc gtcattttct gatccagtag ccatcgcttt gacctgccgc
    65701 tttccgggca tataattacc gcttcatttt tttggcaggg ctttttagat gttatttgca
    65761 tggataaccg atcctaacgc ctggcttgcg ctcggtacgc tgacgctgct ggagatcgtt
    65821 cttgggatcg acaatattat tttcctttct ctggtggtgg caaagcttcc cacagcacaa
    65881 cgtgctcatg cgcgccgtct ggggttggcg ggagccatgg ttatgcgtct ggcgctgctg
    65941 gcatcaatcg cctgggttac gcgcctgaca aatccgcttt ttacaatatt cagtcaggaa
    66001 atttctgccc gtgatttgat tctgcttctg ggtggcttgt tccttatctg gaaagccagc
    66061 aaggaaatcc acgaatccat tgaaggtgaa gaagaagggc tgaaaacacg cgtttcatca
    66121 ttcctcggcg ctatcgtgca gattatgctg ctggatatta tctttagcct cgactcggtg
    66181 attacggctg tgggtctgtc agatcacctg tttattatga tggcagccgt ggtgattgcc
    66241 gtaggcgtga tgatgttcgc cgcgcgctcg attggtgatt ttgtcgaacg ccatccttcg
    66301 gtaaaaatgc tggcgctctc tttcctgatt ctggtcggct ttaccctgat tctggaaagt
    66361 ttcgacatcc acgtaccgaa aggttacatc tacttcgcga tgttcttctc tattgcggtt
    66421 gaaagcctca acctgattcg caacaaaaag aacccgctct gatactccgt acgctctcct
    66481 gcgggagggc gtactcactc ctgttaccct cacagtttaa gattttcctg ctttcaagat
    66541 atataacgtc ggtttataaa cagactatta tcataggtgg gccaaatgag gataaccgga
    66601 tgaaaaaatg ggcagtaata atttctgcag tcggactggc gtttgctgtt tccgggtgtt
    66661 ccagtgatta cgtcatggcg accaaagatg gccgtatgat tttgaccgat ggaaaacctg
    66721 aaattgatga tgataccggg ctggtgagtt atcacgatca gcaaggtaac gcgatgcaaa
    66781 ttaaccgtga tgatgtttcg caaattattg aacgttaaca aataaggtca gcatccggct
    66841 ggccttaaga tttttctctt tcccttttcc cttccctctg ccatttttat attccttatg
    66901 tcgtgattat aaaaaggaaa cggctatgca atatcaccgt ataccccaca gttcgctgga
    66961 agtcagcacg ctggggcttg gcacgatgac gtttggtgaa cagaacagcg aagccgacgc
    67021 ccacgcacaa ctcgactatg ccgtcgctca gggcattaac cttatcgacg ttgccgaaat
    67081 gtacccagta cctccgcgcc ccgaaacgca agggttaacc gaaacctacg tcggcaactg
    67141 gctggcgaaa catggcagcc gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc
    67201 gcgcaataat gacaagggca tccgcccgga tcaggcgctg gatcggaaga atatccgcga
    67261 agcgctgcat gacagcctca agcgcctaca gactgattac ctcgatcttt atcaggtgca
    67321 ctggccgcag cgcccgacca actgcttcgg caaactcggt tatagctgga cggattctgc
    67381 gcctgcggtt tcgctgctgg atacgctgga cgcactggca gagtaccaac gcgcgggaaa
    67441 aattcgttat atcggcgtgt cgaacgaaac tgcatttggc gtaatgcgct acctgcatct
    67501 ggcggacaaa cacgatctgc cgcgtattgt caccattcag aacccttaca gtctgttaaa
    67561 ccgcagtttt gaagtaggtc tggcagaagt cagccagtat gaaggggtcg aactgctggc
    67621 ctattcgtgc ctgggtttcg gcacgctgac cgggaaatat ctcaatggtg caaaacccgc
    67681 tggcgcacgt aatacgctct ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa
    67741 agccgtcgcg gcgtatgttg atatcgccag acgtcatggc ctggaccctg ctcagatggc
    67801 gctcgcgttt gtacgccgtc aaccgtttgt tgccagcact ctgctgggcg caaccacgat
    67861 ggatcagctg aaaactaaca tcgaaagttt gcatctggag ttaagcgaag acgtattagc
    67921 tgaaattgaa gcggtgcatc aggtttatac ttatccggca ccataagatt aacgccggtt
    67981 aaaaccggcg ttaaatatta atgacggcgc tgccagatcc acagcgccgt tattgccagc
    68041 gcaaacagcg caccgaagcc aatgccaatg ggcacgaccg ggatgcctat cattaccgcc
    68101 agcgagtaaa tgcccagcat caacaacatg gcgctgtttt cgccaaggtt ttgtactgca
    68161 atcgcattcc ccgccccgac gcttttttta ccccgctcct gtagcaacgc attgagcgga
    68221 acgacaaaaa agccccccat cacgccaatc agcatcaaca aggcataggc tggcagcagc
    68281 tcgtgttgca gggaaaaaat cagtaccacc acgccaatca aaatcccggc tggcatacag
    68341 cgtgacacgg tttccagcgt aactaacttc gctgccgcac ctgcgccaac cacgatacca
    68401 atcgctacca tcgcgttgag ataggtgggc gtagcgttat cggtaatgcc cagcgccacc
    68461 ggtacccaca gcaccaacag gaaacgcagc gtgacacccg ctccccagaa taaactggtg
    68521 cccaccagcg aaaaacgcgt ttcaccattg cgccatagcg aggtgcaggc attcaggaaa
    68581 ctgcgggtca tgttgatgag attccaggac tgccccggac gcgccgccgc cagtttggga
    68641 atgtagatat tggcaacgac cgcaccaccg taggccagtg cgcatgcggc cagggcgacg
    68701 aggacatgcc agtcagccag cacaccaccg gctacggaac cgagcaaaat cgccgctatg
    68761 gtagaagctt ccattaaacc gttagctttc actaacttac tacccgtggt taattcgccg
    68821 agaataccgt atttcgccgg tgaataggct gcagcaccaa cacccaccag cgtatagccg
    68881 agaaacggat tgataccaaa gcagatactg gctgcgccca gcagcttcag gccgttggca
    68941 aacatcatca cccggccttt ggcgaagcta tccgccacct gcccgacaaa cggcgcaaaa
    69001 agaatgtaag cacctacaaa caccatttgc aggatgggct ggctccactc cggatagaac
    69061 tgcgctttca gtaacgccag agtggcaaac agtagggcat tatcgccaaa cgcagagaga
    69121 aactgcgcca cgataaccgc tttcatcccc ttcgaccaca acgaagtgtt agtgtgcact
    69181 gactcactca tcgtgttgtt ccgcttcgtc tacccagctt ttcaacgtga caaagtcagg
    69241 tttgccgctg ccaagtaatg gcatctgttt cagatagcga atatcgcgcg gtacagcaag
    69301 ctccggcacg ccgtgctcgc gggcatactg ttgcaactta tcgcgcgtca gttcgttatc
    69361 tgtggtgaaa agcaccagtg cctcgccttt gctggcatcg ctcttaatcg cagtggcatg
    69421 gactttatct ggcgaaacac caagtgccag ttgttccacc atttccagcg acaccatttc
    69481 gcctgcaatt ttggcaaagc gttttgcgcg gccctgaatc tgcacaaagc ccagctcgtc
    69541 aaaacgcaca atatcgccag tgtcatacca gccgcgctcc atttcgccgc gaacattctc
    69601 ggcggtgggc acttccagta cacctggctt ctccacccgc agatagccgt tcattatgtt
    69661 cggccctttc agttgcaggc gtccgccctc ttcgatacca gggaccgaca acaggcgcgc
    69721 atccattcct ggtagaatac gccctaccgt accgggtttc gccgccatcg gtacgttgat
    69781 agaaacgaca ggcgcgcatt cggtcacacc gtagccttca aggatgcgca ggccaaattt
    69841 atcctgccaa agctgtttgg tactttcttg tagtttttct gcgcctgcca ccacatagcg
    69901 tagacgatag aagtcatacg ggttggcgaa acgcgcgtag tgaccgagga aagtcgaagt
    69961 gccgaacaac acggtgcaac tgcggtcata caccaactcc ggcacaatac ggtaatgcag
    70021 cgggcttgga taaagaaaca cttctgcacc tgtgagcagt ggcgtaaaca ggcctaccgt
    70081 cagcccaaag gagtgaaaca gcggtaacgc cgacataaag cgatcgttgg tggtgaagtc
    70141 ggcaatcgtt ttaatctgct cgacattcgc cagaatgctt ttatggctat ggacgacgcc
    70201 tttcggatgg ccttcagaac cggaggtaaa aaggatcagc gcctcttctt ccggctgctg
    70261 tttaacctgt gccagacgcg gcatcagcaa atgagcgaag atccatactt tgtcggcagt
    70321 ggtgacatct gcttttaaat cttccagata gacccagcgc acctgagtaa gttgctccgg
    70381 cagatgccag agtttgcctt tatcgagaaa ctggcgggaa gtgaagatgg ttttgatttc
    70441 agccgccgta atagcactgg tcagcccttt taccccggca gtgtagttca tcattgcggg
    70501 aatgcggcga cgggcgatgg ccccaaaaat cactgccgca ctgatgcctg cattgggcag
    70561 cattaagccg atgcgttcgc cttcaacact gtatttttca aggatgcgtc caacaaacag
    70621 cgttttcgta agcaatttgc gataggagtc tggggtaaag ttgacgtctt cgacacattt
    70681 cttcccggct ccgaagcggt acattgcact cagtaaagat tcgtacagcg tttcacgcgg
    70741 gcgcaccgcc attcgcgctt ccatcattat ttgatgcagc atttcgccag cgattttgcg
    70801 acggtcacgg gcacgcggcg catccggcat ctccacctgc gttggtggca aaatatgcag
    70861 agtaatttgc gggaacaagc gacgtttaac cagacctttc aggcggctga agtgcgtgag
    70921 ttccgcccct tcaatacgca caggaataac cgttgcacca gacttcgccg cgacaaaacc
    70981 cgcgccatcg tagattttca tcagcgagcc tgtagtggtg atgcgtcctt cagggaaaat
    71041 caccactggt cggccctgtt ccaccagacg taccagatgt ttaatagcca taggttgcgt
    71101 cgggtcgaga ggaacaaagt cgataaatga tttcagccaa cgcatatacc actgttggct
    71161 tattgaggtg taaacggcaa acactggacg cacaggtaaa aacagtccaa gcaaaatgcc
    71221 atcaataaaa gagacgtgat taggcgtaat tagaacgcgc tcgcccttca gtgcctgggt
    71281 gtcacccgta acgcgaacgc gatacaaaac acggcacaaa tttcgaaaaa agctaaaaag
    71341 cattccaact ccctttgctc tgattcagta aaagcgaatg gagggagatt acacgagata
    71401 aagaacgcga gcgacagtaa attaggtgcg aaaaaaaacc tgcgcatccg cgcaggttgg
    71461 tgcaagagac agggtacgaa gagcgtaccg aataatctca ccaatcaata cctctgggat
    71521 cttgattgtg gtctgcacga cgactcttcg ccagcgagaa aacgcaaagg aatgaaggga
    71581 aatgcaacga ggtgtgtaaa ttgtcggtta ctgttacaga ttgatgaccg gcaaaaaaaa
    71641 acctgcgcat ctgcgcaggc tggtgtaatt catgtgctca acccgaagtt gacttcacct
    71701 atcaatacct ctgggatcac cactttagca acctgaagcc aaacgccacc agcggtcaat
    71761 cgcaacagcg tttcgcaaag tgtaaccaaa ggtttgcaat ctcttttttt gtcttactta
    71821 tttgcgtttg gctcacattc ccacgatgaa aacacgccac cccttgaacc aacgggcgtt
    71881 ttccgtaaca ctgaaagaat gtaagcgttt acccactaag gtattttcat ggcgaccata
    71941 aaggatgtag cccgactggc aggcgtttca gtcgccaccg tttcccgcgt cattaataat
    72001 tcacctaaag ccagtgaagc ttcccggctg gctgtgcata gtgcaatgga gtctcttagc
    72061 tatcacccga acgccaacgc ccgtgcgctg gcgcagcaga ccactgaaac ggtcggtctg
    72121 atcgttggtg atgtttccga tccgtttttc ggtgcaatgg tgaaagcggt cgaacaggtg
    72181 gcttatcaca ccagtaattt cttattgatt ggcaacggtt accacaacga acaaaaagag
    72241 cgtcaggcta ttgagcaact gatccgccat cgctgtgctg cgttggtcgt ccatgccaaa
    72301 atgatcccgg atgccgattt agcctcatta atgaaacaaa tgcccggtat ggtgctgatc
    72361 aaccgtatcc tgcctggctt tgaaaaccgt tgtattgctc ttgacgatcg ttacggtgcc
    72421 tggctggcaa cgcgtcattt aattcagcaa ggtcataccc gcattggtta tctgtgctct
    72481 aaccactcta tttctgacgc cgaagatcgt ctgcaagggt attacgatgc ccttgctgaa
    72541 agtggtattg cggccaatga ccggctggtg acatttggcg aaccagacga aagcggcggc
    72601 gaacaggcaa tgaccgagct tttgggacga ggaagaaatt tcactgcggt agcctgttat
    72661 aacgattcaa tggcggcggg tgcgatgggc gttctcaatg ataatggtat tgatgtaccg
    72721 ggtgagattt cgttaattgg ctttgatgat gtgctggtgt cacgctatgt gcgtccgcgc
    72781 ctgaccaccg tgcgttaccc aatcgtgacg atggcgaccc aggctgccga actggctttg
    72841 gcgctggcgg ataatcgccc tctcccggaa atcactaatg tctttagtcc gacgctggta
    72901 cgtcgtcatt cagtgtcaac tccgtcgctg gaggcaagtc atcatgcaac cagcgactaa
    72961 ccgcagttaa agcaattcca gcgccagtaa ttcttcgatg gtctggcgac ggcgaatcaa
    73021 ccgcgcctga ccattatcaa acagaacctc tggtaacagc ggacggctat tgtagttgga
    73081 tgacattgat gcgccatatg cccctgtatc atgcagtacc agataatcac ctgccttcac
    73141 ttccggcaag gcgcgggttt caacatttcc cccttcctgc tgggtaaaga catcgcccga
    73201 ttcacataac ggtccggcga cgacggtttc caccgttggc gcgtgttcca gagaacgacc
    73261 atcagctgcc agggcactga tatggtggta actaccgtac attgccgggc gcatcagatc
    73321 gttgaaccca gcatcaacca gcacaaagtg gcggctaccc atttgtttga cgctccgcac
    73381 ctgagtaatt aatacgccag actgcgctac caggaagcga cccggttcaa tttccagttt
    73441 cacagggtgg cccaaatggc gggcgatttg ctcacgcgcg gcattccaca gaccataata
    73501 atgttcggta tcaaccgcct cttcaccctg ttgataagga acagaaagcc caccgcccgc
    73561 agaaatagcc tgtaaatcct gaccgaattc gatgacctga cgcaccatag caccacacac
    73621 ctgttccaga tgggcataat caacgccaga accaatgtgc atgtgaatgc cgaccagctg
    73681 cagatgatga cgttgtatca cgtccagtgc ggcgggcaga tcggtgtacc agataccgtg
    73741 cttgctgttt tcgccaccgg tattggtttt ttggctatgt ccgtgaccaa accccggatt
    73801 aacgcgcagc catacccgat gccctggcga aacctggccc agttggtcga gcatatcaac
    73861 agaacccgca ttcaccggaa tttgcaattc actgacgcgt tcaagcgtcg cctgatcgat
    73921 aacatctgcc gtaaaaacaa tatcatcggg gtgcgtttgc ggattgtaac ccgccgccaa
    73981 cgcacgctct atttcgccta acgagacgga atccactttc acgccctgct cacgcattaa
    74041 gcgcaaaata tgaatattgg aacaggcttt ctgtgcaaag cgcaccacat caaactgttt
    74101 cagcgctgca atctgccgac gaataatttg cgcatcgtag acccacaccg ggcagccaaa
    74161 ttcagcgggc aaacgcagca gattttcggc ggtgagatcg gtatcggtgc tgaacagtga
    74221 atgtggcata acaaactcca gataagtgct tttttatgat tacgccacat cataaaaaga
    74281 ataaaaaata tcgatttatg tcgagtttat gcaaaaatga tatggattac cggattgcga
    74341 gagagcgcta atggccgccg ttaacttacg tcatattgaa atttttcatg cggtaatgac
    74401 cgccggaagc ctgactgagg cggcacacct gctacacacc tcacagccaa ccgtcagccg
    74461 cgaactggcg cgctttgaga aggtgatcgg gctgaaattg tttgagcgcg tacgtgggcg
    74521 attacatcct accgtgcaag gactgcgtct gtttgaagaa gtgcaacgat cctggtacgg
    74581 actggatcgc attgtcagcg ccgcagaaag tctgcgcgag tttcgccagg gagaactgtc
    74641 tattgcctgc ctgccggtct tttcgcaatc ttttttaccg cagctcctgc aaccctttct
    74701 ggcacgttat cccgatgtca gcttaaatat cgtgccccag gaatcaccgc tacttgaaga
    74761 gtggctctcg gcccagcgtc atgatttagg actcactgaa acgctccata cgcctgcggg
    74821 aacagaacgt accgaattac tctctttaga tgaagtgtgt gtgttacctc cgggtcatcc
    74881 gctggcggta aaaaaggtat taacgccgga tgattttcag ggtgagaact acatcagcct
    74941 ttcccgtact gacagctatc gccagttgct ggatcagcta tttactgaac atcaggttaa
    75001 acgacgcatg atcgtagaaa cccacagcgc cgcgtcagtc tgcgcaatgg tacgggcggg
    75061 ggtaggtgtt tcggtggtta acccgctcac cgcactggat tatgcggcaa gcggtttagt
    75121 ggtgcggcgg ttcagtattg cggttccgtt caccgtcagc ctgatccgcc ccctgcaccg
    75181 cccgtcatca gcgctggtgc aggcgtttag tgagcattta caagcggggt taccgaaact
    75241 ggtcacttct cttgacgcta ttttgtcgtc agctacgaca gcataaaagc gacagcatcc
    75301 tcggcatgga tcgccgcggt atcaaacaca ggcaaaacac tgcgctcttc tggcaccagt
    75361 aaaccaattt ctgtgcagcc aaaaatgacg ccctgtgcgc cctgttctgc aaggcgagca
    75421 atcacttgcg cataataagc gcgtgacgct tcggtaaatt gccccagaca cagttcttca
    75481 aaaataatct gattaatttt cgcccgttca tccgcttcag gaataagaca attgatggaa
    75541 aattgttccg tcagccgccc gcgataaaaa tcctgttcca tggtgtaacg cgtacccagc
    75601 agcgccacac gagtcattcc ggccccggta attgcacgtc cggtggcatc cgcaatgtgt
    75661 aagaaaggca gagtgcaacg tgactcaatg gcatccgcca ctttatgcat cgtattggta
    75721 cataacacaa taccttctgc gcccgcccgc tgtaagccaa gcgccgcctc agccagaatg
    75781 tccccggttt tatcccattc cccgcgacgc tggcactctt ctatttcatg aaaatcgacg
    75841 ctatgtagca gcacttgcgc agagtgaagc ccaccaagcc gctgtttaat gccttcattt
    75901 atcaaacgat agtaaggaat ggtggattcc cagctcattc ctcccagcaa accaattgtt
    75961 ttcattcctc tctcctgatg tgtgttaccg caacaaggct gaataaggaa cgagacaaac
    76021 gcctcaacgg ccaagtgccc aatctctatt aacgaaaaaa gggccggatg tacagcacat
    76081 ccggcccgtg aaatcagacg ccgatatttc tcaacttctc gcctgccatc agtttgcgtt
    76141 cgatatgttc cagcgtgaca tttttggttt ccggaatgag ccagaaagta atgcccacaa
    76201 acgcaatgtt cagcgcagtg tagagccaga acgtaccggc agcgccaatg ctatcaagca
    76261 gtgtcaggaa ggtcgcgccg ataatcatat tcgacaccca gtttgtcgtc gtcgaacagg
    76321 taataccgaa atcgcggcat ttcagcggct gaatttcaga gcacaggatc cacaccactg
    76381 gcgcggcgct catcgcataa ccggcaatac acatcatcgt catgccaaca gagagccagg
    76441 acaagccact ggaagccgta ccgttatcaa actgcatcag gcaatagccc agcaccagag
    76501 tgcctaacgc catcacgctg aaaccaattt tcagagccgg tttacgacct gctttatcta
    76561 ccgtaaagac cgcaatgaag gtcgcgaaca taaaggtcag tcccacgacc agagtcgcaa
    76621 tcatctgttg ttctgtggtc gtaaagcccg ccattttgaa gatacgcggc gcgtagtaca
    76681 tgatgatgtt cataccggta aactgctgca tcgcctgcaa caacataccg agaaacacag
    76741 cacgacggac gttacggttg atcttaaaca gtgcccaacc gccctgtttt aacttcaggc
    76801 tttcacgaat ttcgttgagt tcttctcgcg ctttttccga cgtatcgcgc agcatacgca
    76861 atacttcttc cgcctcaata tgacgcccct tttccgccag ccagcgcggg ctatttggca
    76921 ggaagaccac cagaataatc agcagaactg ctggtaaagc aagaaccccc aacattgcgc
    76981 gccagttacc gctataactg aacgctgtat cggataaaaa cgccagcacg atgccgagtg
    77041 tgaccatcaa ctggtacata ctgatcatct taccgcgaac gttttcactt gccatttcag
    77101 aaagatacag aggagcggtg taagacgcga tcccgacagc aatgcccagc accacccgag
    77161 cggcgattaa catctctacg ctggtcgcaa aagcggaccc tatagaaccg agtacaaaca
    77221 ggatggcccc cgccatcagg ctgtatttac gccccaggcg gaacgacagc caaccattaa
    77281 acagcgcacc aattgctgcg ccgagcatca tgctactaac cacccattcc tgcaaacgac
    77341 tggtcagcac aaagtgatcg gtaatgaacg gcaacgctcc ggcgattacg ccgatatcaa
    77401 gaccaaataa caatcctgcg accgcagcag ctaccgaaac aaacatattc atacgccgcg
    77461 tatcccgcaa agaacgtggc gttaaagcag attccgtatt gatagtaacc attttttcct
    77521 gccagcagag aataagacat agtgaaaaaa tacgtgaaca attcacacag gtgtcaggtc
    77581 g
//
LOCUS       DAJOWB010000008        72952 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJOWB010000008 DAJOWB010000000
VERSION     DAJOWB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.008"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            52..1818
                     /gene="adeD"
                     /locus_tag="OHO85_000617"
     CDS             52..1818
                     /gene="adeD"
                     /locus_tag="OHO85_000617"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCQ2670041.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQLLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1863..3254)
                     /gene="uhpT"
                     /locus_tag="OHO85_000618"
     CDS             complement(1863..3254)
                     /gene="uhpT"
                     /locus_tag="OHO85_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCQ2670042.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3392..4711)
                     /gene="uhpC"
                     /locus_tag="OHO85_000619"
     CDS             complement(3392..4711)
                     /gene="uhpC"
                     /locus_tag="OHO85_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCQ2670043.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4721..6223)
                     /gene="uhpB"
                     /locus_tag="OHO85_000620"
     CDS             complement(4721..6223)
                     /gene="uhpB"
                     /locus_tag="OHO85_000620"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCQ2670044.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6223..6813)
                     /gene="uhpA"
                     /locus_tag="OHO85_000621"
     CDS             complement(6223..6813)
                     /gene="uhpA"
                     /locus_tag="OHO85_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCQ2670045.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6888..7178)
                     /gene="ilvN"
                     /locus_tag="OHO85_000622"
     CDS             complement(6888..7178)
                     /gene="ilvN"
                     /locus_tag="OHO85_000622"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCQ2670046.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7182..8870)
                     /gene="ilvB"
                     /locus_tag="OHO85_000623"
     CDS             complement(7182..8870)
                     /gene="ilvB"
                     /locus_tag="OHO85_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCQ2670047.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8976..9074)
                     /gene="ivbL"
                     /locus_tag="OHO85_000624"
     CDS             complement(8976..9074)
                     /gene="ivbL"
                     /locus_tag="OHO85_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCQ2670048.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            9639..9728
                     /gene="tisB"
                     /locus_tag="OHO85_000625"
     CDS             9639..9728
                     /gene="tisB"
                     /locus_tag="OHO85_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCQ2670049.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            10147..11331
                     /gene="emrD"
                     /locus_tag="OHO85_000626"
     CDS             10147..11331
                     /gene="emrD"
                     /locus_tag="OHO85_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCQ2670050.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11339..11836)
                     /locus_tag="OHO85_000627"
     CDS             complement(11339..11836)
                     /locus_tag="OHO85_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCQ2670051.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQTADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11833..12195)
                     /locus_tag="OHO85_000628"
     CDS             complement(11833..12195)
                     /locus_tag="OHO85_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCQ2670052.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12185..12532)
                     /locus_tag="OHO85_000629"
     CDS             complement(12185..12532)
                     /locus_tag="OHO85_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCQ2670053.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12642..13091
                     /locus_tag="OHO85_000630"
     CDS             12642..13091
                     /locus_tag="OHO85_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670054.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13138..14631)
                     /locus_tag="OHO85_000631"
     CDS             complement(13138..14631)
                     /locus_tag="OHO85_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HCQ2670055.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELSEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(14628..16343)
                     /locus_tag="OHO85_000632"
     CDS             complement(14628..16343)
                     /locus_tag="OHO85_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCQ2670056.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            16510..17403
                     /locus_tag="OHO85_000633"
     CDS             16510..17403
                     /locus_tag="OHO85_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCQ2670057.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(17400..17576)
                     /locus_tag="OHO85_000634"
                     /pseudo
     CDS             complement(17400..17576)
                     /locus_tag="OHO85_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082801.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            complement(17576..18214)
                     /locus_tag="OHO85_000635"
     CDS             complement(17576..18214)
                     /locus_tag="OHO85_000635"
                     /inference="COORDINATES: protein motif:HMM:NF014148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670058.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQERFPLRALKFYDNDGAR
                     QEVIAEACKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHWWSAISRSFRKG"
     gene            complement(18214..19830)
                     /locus_tag="OHO85_000636"
     CDS             complement(18214..19830)
                     /locus_tag="OHO85_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCQ2670059.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            20126..20842
                     /locus_tag="OHO85_000637"
     CDS             20126..20842
                     /locus_tag="OHO85_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCQ2670060.1"
                     /translation="MIYKSIAERLRIRPNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(20839..22500)
                     /locus_tag="OHO85_000638"
     CDS             complement(20839..22500)
                     /locus_tag="OHO85_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCQ2670061.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(22697..23125)
                     /gene="ibpB"
                     /locus_tag="OHO85_000639"
     CDS             complement(22697..23125)
                     /gene="ibpB"
                     /locus_tag="OHO85_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCQ2670062.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(23237..23650)
                     /gene="ibpA"
                     /locus_tag="OHO85_000640"
     CDS             complement(23237..23650)
                     /gene="ibpA"
                     /locus_tag="OHO85_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCQ2670063.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(23644..23716)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            23956..24288
                     /locus_tag="OHO85_000641"
     CDS             23956..24288
                     /locus_tag="OHO85_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCQ2670064.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(24290..25504)
                     /locus_tag="OHO85_000642"
     CDS             complement(24290..25504)
                     /locus_tag="OHO85_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCQ2670065.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            25605..26669
                     /gene="cbrA"
                     /locus_tag="OHO85_000643"
     CDS             25605..26669
                     /gene="cbrA"
                     /locus_tag="OHO85_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCQ2670066.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            complement(26666..28003)
                     /locus_tag="OHO85_000644"
     CDS             complement(26666..28003)
                     /locus_tag="OHO85_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2670067.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(28078..29226)
                     /gene="dgoD"
                     /locus_tag="OHO85_000645"
     CDS             complement(28078..29226)
                     /gene="dgoD"
                     /locus_tag="OHO85_000645"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HCQ2670068.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(29223..29840)
                     /gene="dgoA"
                     /locus_tag="OHO85_000646"
     CDS             complement(29223..29840)
                     /gene="dgoA"
                     /locus_tag="OHO85_000646"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCQ2670069.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(29824..30702)
                     /gene="dgoK"
                     /locus_tag="OHO85_000647"
     CDS             complement(29824..30702)
                     /gene="dgoK"
                     /locus_tag="OHO85_000647"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCQ2670070.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(30699..31388)
                     /gene="dgoR"
                     /locus_tag="OHO85_000648"
     CDS             complement(30699..31388)
                     /gene="dgoR"
                     /locus_tag="OHO85_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCQ2670071.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            31666..32322
                     /locus_tag="OHO85_000649"
     CDS             31666..32322
                     /locus_tag="OHO85_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670072.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(32368..33180)
                     /gene="yidA"
                     /locus_tag="OHO85_000650"
     CDS             complement(32368..33180)
                     /gene="yidA"
                     /locus_tag="OHO85_000650"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCQ2670073.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(33295..33693)
                     /locus_tag="OHO85_000651"
     CDS             complement(33295..33693)
                     /locus_tag="OHO85_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCQ2670074.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(33933..36347)
                     /gene="gyrB"
                     /locus_tag="OHO85_000652"
     CDS             complement(33933..36347)
                     /gene="gyrB"
                     /locus_tag="OHO85_000652"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCQ2670075.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(36376..37449)
                     /gene="recF"
                     /locus_tag="OHO85_000653"
     CDS             complement(36376..37449)
                     /gene="recF"
                     /locus_tag="OHO85_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCQ2670076.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(37449..38549)
                     /gene="dnaN"
                     /locus_tag="OHO85_000654"
     CDS             complement(37449..38549)
                     /gene="dnaN"
                     /locus_tag="OHO85_000654"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCQ2670077.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(38554..39957)
                     /gene="dnaA"
                     /locus_tag="OHO85_000655"
     CDS             complement(38554..39957)
                     /gene="dnaA"
                     /locus_tag="OHO85_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCQ2670078.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            40254..40334
                     /locus_tag="OHO85_000656"
     CDS             40254..40334
                     /locus_tag="OHO85_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670079.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            40564..40704
                     /gene="rpmH"
                     /locus_tag="OHO85_000657"
     CDS             40564..40704
                     /gene="rpmH"
                     /locus_tag="OHO85_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCQ2670080.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            40721..41080
                     /gene="rnpA"
                     /locus_tag="OHO85_000658"
     CDS             40721..41080
                     /gene="rnpA"
                     /locus_tag="OHO85_000658"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCQ2670081.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            41044..41301
                     /gene="yidD"
                     /locus_tag="OHO85_000659"
     CDS             41044..41301
                     /gene="yidD"
                     /locus_tag="OHO85_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCQ2670082.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            41304..42950
                     /gene="yidC"
                     /locus_tag="OHO85_000660"
     CDS             41304..42950
                     /gene="yidC"
                     /locus_tag="OHO85_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCQ2670083.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            43056..44420
                     /gene="mnmE"
                     /locus_tag="OHO85_000661"
     CDS             43056..44420
                     /gene="mnmE"
                     /locus_tag="OHO85_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCQ2670084.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            44663..44737
                     /gene="tnaC"
                     /locus_tag="OHO85_000662"
     CDS             44663..44737
                     /gene="tnaC"
                     /locus_tag="OHO85_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCQ2670085.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHSP"
     gene            44958..46373
                     /gene="tnaA"
                     /locus_tag="OHO85_000663"
     CDS             44958..46373
                     /gene="tnaA"
                     /locus_tag="OHO85_000663"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCQ2670086.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            46464..47711
                     /gene="tnaB"
                     /locus_tag="OHO85_000664"
     CDS             46464..47711
                     /gene="tnaB"
                     /locus_tag="OHO85_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCQ2670087.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            47843..49018
                     /gene="mdtL"
                     /locus_tag="OHO85_000665"
     CDS             47843..49018
                     /gene="mdtL"
                     /locus_tag="OHO85_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCQ2670088.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPAAPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            48993..49952
                     /gene="yidZ"
                     /locus_tag="OHO85_000666"
     CDS             48993..49952
                     /gene="yidZ"
                     /locus_tag="OHO85_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCQ2670089.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKQIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWSLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            50109..50858
                     /locus_tag="OHO85_000667"
     CDS             50109..50858
                     /locus_tag="OHO85_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCQ2670090.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPLHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            50880..51446
                     /gene="yieF"
                     /locus_tag="OHO85_000668"
                     /gene_synonym="chrR"
     CDS             50880..51446
                     /gene="yieF"
                     /locus_tag="OHO85_000668"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCQ2670091.1"
                     /translation="MSEKLKVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(51500..52837)
                     /gene="adeP"
                     /locus_tag="OHO85_000669"
     CDS             complement(51500..52837)
                     /gene="adeP"
                     /locus_tag="OHO85_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCQ2670092.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALMFILKIVFIDAH"
     gene            53004..53669
                     /gene="yieH"
                     /locus_tag="OHO85_000670"
     CDS             53004..53669
                     /gene="yieH"
                     /locus_tag="OHO85_000670"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCQ2670093.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            53736..54209
                     /locus_tag="OHO85_000671"
     CDS             53736..54209
                     /locus_tag="OHO85_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670094.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELYENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            54258..54845
                     /locus_tag="OHO85_000672"
     CDS             54258..54845
                     /locus_tag="OHO85_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCQ2670095.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKRGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(54907..55629)
                     /locus_tag="OHO85_000673"
     CDS             complement(54907..55629)
                     /locus_tag="OHO85_000673"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCQ2670096.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(55644..56813)
                     /locus_tag="OHO85_000674"
     CDS             complement(55644..56813)
                     /locus_tag="OHO85_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCQ2670097.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(56840..58456)
                     /gene="bglH"
                     /locus_tag="OHO85_000675"
     CDS             complement(56840..58456)
                     /gene="bglH"
                     /locus_tag="OHO85_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCQ2670098.1"
                     /translation="MFRRNLITSAVLLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(58542..59936)
                     /gene="bglB"
                     /locus_tag="OHO85_000676"
     CDS             complement(58542..59936)
                     /gene="bglB"
                     /locus_tag="OHO85_000676"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCQ2670099.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(59955..61832)
                     /gene="bglF"
                     /locus_tag="OHO85_000677"
     CDS             complement(59955..61832)
                     /gene="bglF"
                     /locus_tag="OHO85_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCQ2670100.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLDEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTSLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(61965..62801)
                     /gene="bglG"
                     /locus_tag="OHO85_000678"
     CDS             complement(61965..62801)
                     /gene="bglG"
                     /locus_tag="OHO85_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCQ2670101.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILG
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVHK
                     EH"
     gene            complement(63087..63812)
                     /gene="phoU"
                     /locus_tag="OHO85_000679"
     CDS             complement(63087..63812)
                     /gene="phoU"
                     /locus_tag="OHO85_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCQ2670102.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(63827..64600)
                     /gene="pstB"
                     /locus_tag="OHO85_000680"
     CDS             complement(63827..64600)
                     /gene="pstB"
                     /locus_tag="OHO85_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCQ2670103.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(64691..65581)
                     /gene="pstA"
                     /locus_tag="OHO85_000681"
     CDS             complement(64691..65581)
                     /gene="pstA"
                     /locus_tag="OHO85_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCQ2670104.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(65581..66540)
                     /gene="pstC"
                     /locus_tag="OHO85_000682"
     CDS             complement(65581..66540)
                     /gene="pstC"
                     /locus_tag="OHO85_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCQ2670105.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(66627..67667)
                     /gene="pstS"
                     /locus_tag="OHO85_000683"
     CDS             complement(66627..67667)
                     /gene="pstS"
                     /locus_tag="OHO85_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCQ2670106.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(67915..68988)
                     /locus_tag="OHO85_000684"
     CDS             complement(67915..68988)
                     /locus_tag="OHO85_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2670107.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(68999..71521)
                     /locus_tag="OHO85_000685"
     CDS             complement(68999..71521)
                     /locus_tag="OHO85_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCQ2670108.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(71546..72277)
                     /locus_tag="OHO85_000686"
     CDS             complement(71546..72277)
                     /locus_tag="OHO85_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCQ2670109.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(72325..72897)
                     /locus_tag="OHO85_000687"
     CDS             complement(72325..72897)
                     /locus_tag="OHO85_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCQ2670110.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT        18017 a        19191 c        18056 g        17688 t
ORIGIN      
        1 tttctttatg ataaataata atcaaattga taaaatcaaa atgagaaaaa tatgaataat
       61 tctattaacc ataaatttca tcacattagc cgggctgaat accaggaatt gttagccgtt
      121 tcccgtggcg acgctgttgc cgattatatt attgataatg tctctattct cgacctgatc
      181 aatggcggag aaatttccgg cccaattgtg attaaaggac gttacattgc cggtgttggc
      241 gcagaatacg ctgatgctcc ggctttgcag cggattgatg ctcgcggcgc aacggcggtg
      301 ccagggttta ttgatgctca cctgcatatt gaatccagca tgatgacgcc ggtcactttt
      361 gaaaccgcta ccctgccgcg cggcctgacg accgttattt gcgaccctca tgaaatcgtc
      421 aacgtgatgg gagaagccgg attcgcctgg tttgcccgct gtgccgaaca ggcaaggcaa
      481 aaccagtact tacaggtcag ctcttgcgta cccgccctgg aaggctgcga tgttaacggt
      541 gccagtttta cccttgaaca gatgctcgcc tggcgggacc atccgcaggt taccggcctt
      601 gcagaaatga tggactaccc tggcgtaatt agcgggcaga atgcgctgct cgataaactg
      661 gatgcatttc gccacctgac gctggacggt cactgcccgg gtttgggtgg taaagaactt
      721 aacgcctata ttactgcggg tattgaaaac tgccacgaaa gttatcagct ggaagaagga
      781 cgccggaaat tacaactcgg catgtcgttg atgatccgcg aagggtccgc tgcccgcaat
      841 ctcaacgcgc tggcaccgtt gatcaacgaa tttaacagcc cgcaatgcat gctctgtacc
      901 gatgaccgta acccgtggga gatcgcccat gaaggacaca tcgatgcctt aattcgccgc
      961 ctgatcgaac aacacaatgt gccgctgcat gtggcatatc gcgtcgccag ctggtcgacg
     1021 gcgcgccact ttggtctgaa tcacctcggc ttactggcac ccggcaagca ggccgatatc
     1081 gtcctgttga gcgatgcgcg taaggtcacg gtgcagcagg tactggtgaa aggcgagccg
     1141 attgatgcgc aaaccttaca ggcggaagag tcggcgagac tggcacaatc cgctccgcca
     1201 tatggcaaca ccattgcccg ccagccagtt tccgccagcg actttgccct gcaatttacg
     1261 cccggaaaac gctatcgggt cattgacgtc atccataacg aattgattac gcactcccac
     1321 tccagcgtct acagcgaaaa tggttttgat cgcgatgatg tgagctttat tgccgtactt
     1381 gagcgttacg ggcaacggct ggctccggct tgtggtttgc ttggcggctt tggactgaat
     1441 gaaggtgcgc tggctgcgac ggtcagccat gacagccata atattgtggt gatcggtcgc
     1501 agtgccgaag agatggcgct ggcggtcaat caggtgattc aggatggcgg cgggctgtgc
     1561 gtggtacgta acggccaggt ccaaagtcat ctgccgttgc ccattgccgg cctgatgagc
     1621 accgacacgg cgcagttgct ggcggaacaa attgacgcct tgaaagccgc cgcccgtgaa
     1681 tgcggtccgt tacccgatga gccgtttatt cagatggcgt ttctttctct gccagtgatc
     1741 cccgcgctaa aactaaccag tcaggggcta tttgatggcg agaagtttgc cttcactacg
     1801 ctggaagtca cggaataata aaaaagcccg gcgtcatgcc gggcaaaagt caccagttac
     1861 gtttatgcca ctgtcaactg ctgaattttt ttctcgcggc ggattttgcg ttcttccatc
     1921 accgccacta tcgccatcag gcagatacaa ccaatcgcgg cgatatcaag tgcggcgaag
     1981 gtgcctgccc agccggtaag gccgaatacc ggcgtcccat cggcaatcat tcccagacct
     2041 aacttggcaa agctgtcacc aatcaggtaa gcaaaggtgc ctttaatacc atcggcagcg
     2101 ccaatcgctt ttttaggtac aaagccaaca gcagccacac caatcaacaa ttgcgggcca
     2161 aagaccagga aacccaacgc aaagagagaa gccagataga tatattcgtt gctggcatgt
     2221 tgatacacac cgagcgtggc gataatcagc gccagcgcga tgcaggccac caggccacgg
     2281 cgaccgttcg ccaggtcaga gagccagccc cacagcagcg taccgaccag cgcaccagct
     2341 tcaaacagcg taaagccctg aatcgccacc gctttagaga gtttcagttc ctggaacgcg
     2401 tatacggttg accactggtc gataccaata cgtaccacat agaggaaaat gttggcgaag
     2461 cacagcagcc agatcacttt gtttttcagc acatactcaa caaagatctg ccacttggtc
     2521 atatcggtag attctgtctc tttgtcctct tcgctgatct cctcgccgaa cagttcttca
     2581 gctttgccga ggccataaga ttccggggag tcgctaccgt aacgcaggcc gataaaaccg
     2641 acaatcagcg cgataatcga cgggaagata aacatgccga tgacatggcc atcgaacagg
     2701 taatttgccc cgaacagcgc cacacctgct gcgcctgcac cgccaaggtt gtgagaaata
     2761 ttccagaaac cgaggaatgt cccgcgttta cgacgcggcg tccatttggt gatggtggag
     2821 taactgcacg aaccgccggt actctggaaa aagccgctta aggcgtagaa ggcaatcatc
     2881 aggaacaggc taaccgagcc gctgcccata ctggcactga agcccagcat acaaatggca
     2941 gacaggataa gcataaacgg caggaattgt ttggtgtttt tgccgtcggc gtagtaggaa
     3001 accagcgttt tacccacgcc ataagtgatg gagaaaccca ggccgatcat ccccagctgc
     3061 gtcatgctca acccgtaggt cgaaatcatg tcgttctgcg cgatgttaaa gttcttgcga
     3121 atcaggtaca tcgtcaggta gccgataaag accaccaggt aggattgcat gaacggtttg
     3181 aaccacattt tgcgccgcac ttcgagcgga aggtccaggg tcggcttgcg aacctggttt
     3241 aagaaagcca gcatgggtta ctcctgaaat gaatacctgc gtgaggcatg cattgtaaaa
     3301 atcagcagca aatcaggcct gagatagcgt ccaggcaaaa cctggaaaat ttcttagttt
     3361 tgccggatat gaagtgaaaa ggtgagatgc atcacgcttc gcgcggtgtc tgggcgttca
     3421 aaaagggcag taacagcagt gcggaaatcc cggcggcgat agagataacc acaaaaaatc
     3481 cgctccagtg ccaggtatcg agtactttcg ccagcggcca accagcaagc gacgccccca
     3541 gataagcaaa caagccgaca aaccccgtcg ccgcccctgc cgcctctttg tgggaacact
     3601 ctgccgccgc cataccgatt aacatctgtg ggccaaagac aaaaaaacca atggtgaaga
     3661 agcaggttgc ctgcatcacg tagctggcaa atggcatcag ccacagggag ccgactgaaa
     3721 gcaaaattcc ggcggcgaaa atcaaattca tcggccctcg gttgccgtta aacaatttgt
     3781 ccgagcccca acccgctacc agcgcaccga taaatccacc cagttcaaac atcgtcactg
     3841 ccgtattcgc cgtgaccaga tcgacgccca gcgtctcgga catatacaaa ttgccccagt
     3901 cgttgatcgc cgcccggacc acatagacca gcacatagca aaacgaaagc agccagatat
     3961 acggattcag caacacatat ttggtgagga tctctttacg cgtcagccct gccccttctt
     4021 gttgttgagc tatctccagc gcgtcgtgtc gccattcacc gaccgccggt aaacctaacg
     4081 cctgcgggcg atcgcgcagc cgccagcaga gaaaaattcc cacgacgatc gccatacaac
     4141 cagcaatcat catcccggca cgccagccgt aatgtagtgc agccgctgcc atcacaatgg
     4201 gaatgagtgc gccgccgacg ttatgcgccg tgttccataa tgcccaccaa ccgccgcgct
     4261 cggtacgtga ataccaggcc gttaacagac gcgcacacac cggtgaaccc cagccctgga
     4321 aaaaggcgtt cagcacccag agcacggcaa acgcccatag cgacgtcgag aagccaaaca
     4381 gaatgttgat aatgcccgtg gcgataagcc ctatccccat aaaataacgg gcatttgagc
     4441 gatcgctgac aatgccggag acaaacttcg acacgccata ggtaatgtaa aacagggtcg
     4501 ctaacaggcc gatatcgcta cggctgagca cgccgttagc aaggatttct ggtacggcgg
     4561 cgttaaaact tttccgcgtg aagtaaaaca gcgcataacc cagccagatg gtcagcagaa
     4621 tatgccgacg ccagtagcga tagcgggcat caatttcata tttatcagtc attaatggcg
     4681 catcggcagg cgctttcaga aacggcaaca tcatcaaacc ttagacatag cgttgaggta
     4741 gagaaacgct gacacgcgtg ccgtgcagac aggaaatgtg taatgtgcca cccagcgccg
     4801 ttacgcgctc gcgcattccg gtgaggccaa aaccttgttg cccggaaccc ggcggcaaac
     4861 cgctgccatc gtcttcaata accagcatca accgttcatc ctgctgccag ccttgcagcg
     4921 tgaccgcgct ggcatcagca tgtttcacaa tgttgttcag cccttcctgg cagacacgaa
     4981 acagcgtcac gcgctggttt tcacttaatg ctgattcatc gattcgccat tcgagatggc
     5041 tgacaatacc gcgcccttcc agctccattt cccgcatcag tgagcggatg gcctgctcca
     5101 gggtgagatc atccaactgg cgcggacgta accgacccaa caaacggcgc accgcgtcgt
     5161 aaacgcccag cgatagttgt tcgatgagct gcccgctctg cttcacgctg gcgttatctg
     5221 ccgccagccg ctgaacaatg cccgcctgag tacgaatagc agtgatggtc tgaccgatat
     5281 catcatgcag ctcacgcgcc acatcacggc gcacgctctc ttcggtttcc agcaaccgtt
     5341 cagccagatg ctgattgcgc gccagttcct tttgcagcga ctggttaagt tcacgcaacc
     5401 gctggatgcc agcgcccagt aacaaccctg tcagactttg caccagcaac gagagcaata
     5461 aatccaccgg atgatcgcgc caggtttgac tggcgatcag cgcgatggcg ttcatcaacg
     5521 tcgcgatcag cgccccttgc caaccgtagt gccaggccag cgcgataatc ggcagcgcca
     5581 ggcagaatgg cgtaaagcgc gacagttcgt ccggcaatcc caactggagc cagagactaa
     5641 taacaaacaa cagcaagtac cagaccagat gccgcccgcg ccagttgatc ggttgagaaa
     5701 ccagtgacgg gccgagcggc agccaggtgt tattggcgag atagtgccag aacaccagac
     5761 atatcggggc cagcgtcagg ccgccagtta aagtcagcaa cagcgcattc cacgactctt
     5821 tgccgtgcca aagccagggc agcgactgca acaacgccgc cgccgttaac gccgccccct
     5881 gtaacagcaa ggtgcgccag tcacgctgat ggcgatagcg cgagatcagc gctacgggca
     5941 gtaacgtcag taaactaccg atcatcaata acggaaaatg ggttaaaccg accgcctgcg
     6001 ttagccagta aatcagcagc cactccgcac ccagcaatac gggccagtat ccgcgcgggc
     6061 attgcagcat tagccccaga cgcagaccaa acggaaataa cagcaccgcc atatcagggc
     6121 gctcaaccag atgcaggctg atactccaca ggcaaaacca tgcggcagag aagataaaaa
     6181 agcaggcaat aacggtaatt aagcgggaga acaacgtctt catcaccagc catcaaacat
     6241 gcggcgcgcc agctctacgt cgttactgac acccagtttt tccatcaggt tggcgcgatg
     6301 gacgtgcacc gttttcggcg acaagcccag ttcggcagca atctctttca ccgccattcc
     6361 ttgcgccagt ttttccgcca cctgccgttc gcgtttggtt agcgggtcct gacgaccgga
     6421 tgccagttta atggcaatat ccggcgtcag ataacagccg cccgtggcaa ccgtatgcac
     6481 cgccgcaatc agttcgtcag ggctacagcg tttggagaga aagccgcgtg cccccgcgtt
     6541 aagcgcctgc tcaaccagcg ccggactgtc atgaacagag agcattattg tcgccatacc
     6601 tttcggcagc tggcttagca gttccagacc ggagatatcg ggcatggaga tatcgcaaat
     6661 acacacctgc acaccgcgcc ccggcagccc cgccagcgcc tcgcgccccg aaccaaactc
     6721 ggcaactacc tgcaaatcag gttccagccc cagcagctgc gcaaagccgg agcggacgat
     6781 gaggtgatcg tctataaggg caacggtgat catggtcttg tcctggcggg taaaaaaaaa
     6841 cgcgcttacc ttaacgataa gcgcgatgtt gttcaagcct tgagcggtta ctgaaaaaac
     6901 accgcaatct tgttaaacat agtcggatcg gactgattac gctgcacttt caccacatct
     6961 tccagcttat cgatttggct tatcatctgc tccagacgct ggtcgtcatt gaccagtagc
     7021 cagatatggc ttttgtcgct gtcctgaatc ggcagacaaa gaatgccttc aacgttaaaa
     7081 gcgcggcggg caaaaaggcc acaaacgtgg gtcattacgc ccggatggtt gcgaacggtg
     7141 agctccagaa tcacgttgtc atgagttgtg ttttgcatgg cttattcccc caccatttca
     7201 gtattcgccg cacctggcgg caccatcggg taaacttttt cttcggcatc aatgcgcaca
     7261 tggatcagcg ccgggccagg gcgattgatg atttcctgca atgcagcctg cggatcggct
     7321 tcgttattca aatcacaggt ttcgaggccg aatccggcgg caatctgcat aaagttgatt
     7381 tttcccggat aggtggcggc aaaaacgcct tgctcgtaga acagactctg ttgctgatgc
     7441 accagcccca gcgcttcgtt gttcatcaga atgattttga catccagctg attttcactg
     7501 gcggtcgcca tctcctgaat attcatcatc aggctgccgt cgccggagaa acacaacact
     7561 ttgcgatccg ggttcgccag cgccgcgcca atcgccgcag gcaggccaaa acccatcgtg
     7621 cccagcccac cggaggtcag ccactggcgt gggcgattga gcggataagc ttgcgcggtc
     7681 cacatctgat gctgacccac atcggtggtg ataatcgcat tgtcatcgac acaggcggca
     7741 acggcgttga tcaggccgta atggcttaat ggatcgcacg ctttcgggat tggacacggg
     7801 aattcgcgct gcaaatccgc taccaactgg tgccactctg cacgcggttg cgcttccacc
     7861 tgcgggatca attgcgccag cacgtcatca acatccgcct gaatcgccac atgcggctgc
     7921 ttgattttac ccagctctgc gcgatcgata tcgacatgaa taattttggc attcggacag
     7981 aactgctcgg ttttgccaat cgcccggtca tcaaaacgcg caccgagcac tatcaacaaa
     8041 tccgcctcct gcaaaatata gttggtgctg cgcacgccgt gcatccccag catacccagc
     8101 gacaacggat gcgcttttgg caacatgccc agcgccatta aagtcatggt ggtaggcagt
     8161 tgcgctttct ccgccagttc acgcacccgt gcgggcgcat tgatcacacc gccgcccaga
     8221 taaagcaccg ggcgtttggc agcgttaatc atcgccgctg cgtcacgaat gctttcttcg
     8281 ctaaaggcgg gggcggcggc tttttctgcc atagcgggct gtgtttcaat ctcaaaaact
     8341 gccgtttgca catccttagg aatgtctatc cacaccgggc ctgggcggcc tgattgcgca
     8401 atgcggaagg catcgctcat gacctgcggg agttcttcga tatgtctgac cagatagttg
     8461 tgtttggtga tggggataga gatgccgtag gtgtccactt cctggaaggc gtcggtgccg
     8521 atcatcgagg cgggaacctg accggtgatg caaatcagcg ggatggagtc cagccgcgca
     8581 tcggcaatgg cggtcaccag gttagtcgca cccggtccgc tacaggccat acagaccgcc
     8641 ggtttaccgt cggtgcgcgc catcccctga gcgataaatc ccgcgccctg ttcatgacgg
     8701 gccagaatat ggcggatttg cgtgctttgg cttaaggcat cgtaaacagg caggatagaa
     8761 ccgcccggaa tgcccgtcac aatcttaatg ccctgctgtt ccaggaaatg aacgataaat
     8821 tctgcgccgg taaagcgctt acgcgtcgat gttgtgcccg aacttgccat gctccagtcc
     8881 ttttcttctg ggccgacttt ccgggaggtg cttaaacgaa aaaccccgcc cggtttgcgc
     8941 cggcggggtt ttggaatcgt gtgttgttcc agtccctacg gcgcattgcc gacgaccacc
     9001 accacacgca cgacgaccac tgcggcggat agcgcagttg gtagtagttt tgcgttgaac
     9061 atggaagtag tcattgggga ccttgttggt tttgtgttta acaatattta tacaagcaca
     9121 gctttacagg ggagacaatg gaaaattttt cagcaaggga aaattgaggg gctgatcacg
     9181 ttttgtactg aattgcaggt aacaaaaaac cccgccggag cgaggtttca tcagccgcct
     9241 gcggctggta accgcaaagc acactgtatt atgtcaacac tgaaagtata cgtgttccgc
     9301 gcagaacgcg caatttcggc acgaattttg acgtatttag ggcatagttg agtatcgatc
     9361 acagtttgcg ttttgtccaa atattactgt ttatttatac agtaaacttc tataatatca
     9421 ctgtacgcaa tgtgttatgc gggggccgca tcgttacccg gcgcactaag tcctggctga
     9481 aacgggtggt gccgtcagcg ccttaacccc gcgtgagcac actgtgttat gtcaacaagc
     9541 acaacgtttc tccttaagat accgcgtgca caacagctgg caacaggcag cggaaaggta
     9601 cgtcagctgg cagtgctcct gaaccacagg agacgcgtat gagcctggtg gatatcgcca
     9661 ttcttatcct caaactcatt gttgcagcac tgcaactgct tgatgctgtt ctgaaatacc
     9721 tgaagtaatt cagattcaag tcgcaccaaa ggggagcggg taaccgctcc ccttttatat
     9781 ttagcgtgcg ggttggagtt gggggcttct tctaccagca gaatgtttgc acctgcgctg
     9841 cctgtgcccc tcaccctagc cctctcccca aaggggcgag gggaccgtcc cgtgcaatgt
     9901 ttagctcatt ttctgaaagg gaacggtggt ttccccctcg cccctttggg gagagggtta
     9961 cagttcgcac gtaatccctc ccggcaacat ttattgccgc ttttgtttac atattctgcc
    10021 gctaaacaat tccccattcc tggcgtatat ctggctaaca ttcatcaatg tgatagattc
    10081 ctctcccgca tttatgggaa tgcgtagtga cttattctaa ttatttttat aaaagcatcc
    10141 gtgataatga aaaggcaaag aaacgtcaat ttgttattga tgttggtatt actcgtggcc
    10201 gtcggtcaga tggcgcaaac catttatatt ccagctattg ccgatatggc gcgcgatctc
    10261 aacgtccgtg aaggggcggt gcagagcgta atgggcgctt atctgctgac ttacggtgtc
    10321 tcacagctgt tttatggccc gatttccgac cgcgtgggcc gccgaccggt gatcctcgtc
    10381 ggaatgtcca tttttatgct ggcaacgctg gtcgcggtca cgacctccag tttgacggtg
    10441 ttgattgccg ccagcgcgat gcaggggatg ggcaccggcg ttggcggcgt aatggcgcgt
    10501 actttgccgc gagatttata tgaacggaca cagttgcgcc atgctaacag cctgttaaac
    10561 atggggattc tcgtcagtcc gttgctcgca ccgctaatcg gcggtctgct ggatacgatg
    10621 tggaactggc gcgcctgtta tctctttttg ttggttcttt gtgctggtgt gaccttcagt
    10681 atggcccgct ggatgccgga aacgcgtccg gtcgatgcac cgcgcacgcg cctgcttacc
    10741 agttataaaa cgcttttcgg taacagcggt tttaactgtt atttgctgat gctgattggc
    10801 ggtctggccg ggattgccgc ctttgaagcc tgctccggcg tgctgatggg cgcggtgtta
    10861 gggctgagca gtatgacggt cagtattttg tttattctgc cgattccggc agcgtttttt
    10921 ggcgcatggt ttgccggacg tcccaataaa cgcttctcca cgttaatgtg gcagtcggtt
    10981 atctgctgcc tgctggctgg cttgctgatg tggatccccg actggtttgg cgtgatgaat
    11041 gtctggacgc tgctcgttcc cgccgcgctg ttctttttcg gtgccgggat gctgtttccg
    11101 ctggcgacca gcggcgcgat ggagccgttc cccttcctgg cgggcacggc tggcgcgctg
    11161 gtcggcggtc tgcaaaacat tggttccggc gtgctggcgt cgctctctgc gatgttgccg
    11221 caaaccggtc agggcagcct ggggttgttg atgaccttaa tgggattgtt gatcgtgctg
    11281 tgctggctgc cgctggcgac gcggatgtcg catcaggggc agcccgttta agcgcacgtc
    11341 accgcagcat cgtcatcagc tccatgggag aacgatgctg ctttatcaga tcacgcatca
    11401 cccgcatatg cggtgcggag taagaataaa acgcctgata gcccgcacaa agcacgctgt
    11461 ctggcgtctc ctggcgagcg tgcgggcaca ggcgatgcca gtcgcacgcc tggcagtcac
    11521 ggcgcggttg cgggttttcg ctgccacacc atttttgtaa ggtggcgtca aaaagctcaa
    11581 tcgagatacg actgatatct gcccgcaccc aacactcgaa cagggcaatt aaaaatttcc
    11641 cccacagctc tgctgtcacc gaagcggccg tcagatcgcc ccggtcatca aattcaagat
    11701 agggaatgag ctgtacggag cggatgcccc gctgtttgaa attgtgataa aaatcggcag
    11761 tttgcagagc atccttgtac tctacaacca gtttatgcct gtcctcttca gcatcaatga
    11821 cctgacttcc tgtcatgcga ctctctcaat aaatacgata agttgatgta tatgcgttac
    11881 agcaaacagt attgcgagag ataacaccgc gagggagatc aaaaatttgt cacggactac
    11941 gtgaaattgg gaaaaatcgc tattcgtgac atccattaaa ttgcgattac gggtgtagtg
    12001 ccagaggatc agcgccacga tggcgagaat gccaatcgaa atccagaata acatgcccgc
    12061 ctggtgccag ttgtgtttga tagccaacgc catcaacgcg ccgtagccca gcatggtacg
    12121 aaaccacgcc agtgatgtgc gctccggctg cagtcctggg tcggcaatgc gtctggcttt
    12181 gcggctatcc ggcatacaac accagtccca taacgatcac cgccacgacc atcaatatta
    12241 agctaatgat taataagctg ttggtgtagg gcaggtcctc tttcagacgc atcgcttttt
    12301 cattgcgtaa ccagcgcagg tagccataca tcgctaaccc gccggaaaac agacacaaca
    12361 acaacgccag cagttcacga atgacgggcg tggcgaaatc tggcgcaagt tgatcaagcc
    12421 caacccccgc cgccagaaat cctagtgcgg tacggatcca cgccagaaaa gtacgctcat
    12481 tagccagcga gaagcggtaa tcgggcgctt ctccaaggcg ggaaattttc attgttgctc
    12541 ctttgcaatt tcactaacgc tctaccttta gcggtttttt tttaagaatt tcgtccgccc
    12601 gtttcctgat atgctttcgc catccgctac aaggaggtca gatgggtatc atagctcaaa
    12661 ataaaatcag ttcattgggc atgctgttcg gtgccatcgc tttaatgatg gggattatcc
    12721 acttttcatt cggccctttt tccgcaccac cgccaacgtt tgaaagcatc gtggcggaca
    12781 aaacggctga aattaaaaga ggtcttctcg ccggaataaa aggcgaaaaa attaccaccg
    12841 tggagaaaaa agaggacgta gatgtcgata aaatcctcga tcaaagcggt atcgcactgg
    12901 ccattgctgc gctactttgc gcatttattg gcggtatgcg taaggaaaat cgctggggca
    12961 ttcgcggcgc gctggttttt ggcggcggga cgctggcttt ccataccttg ctttttggta
    13021 tcggcatcgt gtgcagcatt ttgctgatat tcctgatttt ttccttcctg accggcggtt
    13081 cactggttta agcagcctga ataatgcccg ccgggtgttc atcagcgggc attatcgtca
    13141 gaacttctgt tttttctctt ccactttcac tccttgcgtc ggtaggccgg tgtcatagtc
    13201 acgtaccacc ggcgaatagc catcttgtgg gcgtggacga aatgccccca tccagcgcgg
    13261 cagtgcatct ttacgccacg gacgcaggct ccattggtaa ctgcggaacg gatcgcgaat
    13321 tttgtccatg taatccaata aggcgtcatg cattttgctg cgaacgtctg caaaacggat
    13381 atcatcgatc aggttatgca tttcatttgg gtcattacgg cgatcgtaaa gttcatcact
    13441 ggtgaagagg ttgagtacca gtttaaagtc atccgtcacc cagcaacgca ccggaataaa
    13501 accgccaaag ctgtcatgct caatctcgta gcggttaaat tccaccatta caccgcgtgg
    13561 ctctttcacg gcaaggatat tttcccccgg cagaatctct ggtttttcaa tatctgccag
    13621 cgccatcatt gtcggcagta aatcgatatg actgactggc gtatcgacct gtcgccgctc
    13681 cccttgcggc gaacggatga tcagcggaat acgggtgatg tcgtcataca tcgccgctcc
    13741 tttactgatc agcttatgtg cgcccatcat ttcgccgtga tcggaagtat aaatgaccca
    13801 cgtattttca cgttgctctg gcgttaaggc attgatgacc cgtccgattt ggtcatcaac
    13861 aaagtcatta caggcaaaat agagcggatg gtgataaagc ccgtcatcac cgactggcga
    13921 tggcatcgcc tgcgcccata agcggtgatg ttccggtttg ttcgccaggt catcctgagc
    13981 tttctcgccc agatcgtagt aaaaatcagc gtatttctct aaatactcca ccggacaggt
    14041 gaacgggtga tgcggctcat catacgaaac caccatcagg aagggttcct cggcgcgcgc
    14101 gggctgttgc agaaaatcta ccgcccgatt gctgatgcgg tgcgcccagg tgaaggtttc
    14161 gtcgatatgg ttcgcctgta aatcctcaac gctgtttagg ccattgcgcc acaggctgat
    14221 ctctttttcc gacagttcgc taaggtagtt cgccccatcg aaccagtaat cagcgtccca
    14281 ctccggcgga cactcgccag tgccgaaata gtcatgaccg tcgagatgcc atttgccgat
    14341 gtaacaggtg tgatagccgg catccttaaa gtagcgcccc atagtggaga tgtttttgcc
    14401 tggcgcgacg ttgttggtcc acgggccgga ctggttagcg tagataccgg taaatagtcc
    14461 ggcgcgtgca ggcgtacaaa ccggtgaaca ggtgtaggcg gaattaaagc gaataccttc
    14521 cgccgccaga ctatcaatat tttgcgtatt cagcggttta ccgctatagc aaccgaccat
    14581 attggtggcc tgggtatcgg tcatgatgaa cagaaaattg gggcgtttca taggctatcc
    14641 ttcgcgtcgg gagtaaaggt tacggctggg tcgtgacgat gccgccagct gtcaaaaatc
    14701 aggtagacaa tcactacggc ggcaatgaaa taactgatca tcgctaacca gcgcgtaccg
    14761 tagccgccga attcagccag cccggcatag acgccaatca tcgcgaacag gatgccaatt
    14821 gacgcgatct tgacgtttct ccacggtttc atgtctaccg caaacgcatc tttgaaggtg
    14881 aacggcgtgg cgcgcggttt gataaaaccg atcaccagca tcacgaccac gttgatgcag
    14941 aacgtacagg ccagcacata gaggaaatgg aagtcgaact tcaccagata gttgatggtg
    15001 atgtagctga ttatgccaat ccccatcgcc acttttgccg ccagcgccgg gatgcgcggg
    15061 aagaaaaagc ccataatgat gatggtaacc agcggcacgt tgtaaatgcc gttgagctgt
    15121 ttcatccagc tatacagccc ctgcggcgcg ttggcgatcc acggcgcgac cagcaccgaa
    15181 acgatagcga taaagaaacc aaatttgcgc ccgacggtga ccagctgctg cggctcggca
    15241 ttctggttaa tgatgcgacg gtaaataccc atactgaata acgtactggc gctattcaga
    15301 aagccgttga aggtactgat caccgcacca aataacaccg cgccgaagaa acccaccatt
    15361 ggcactggca gaacgttatt gaccagcgtc gggtaggcca tgtcggcttt cggtaaatcc
    15421 tgatacagat gaaacgcaat caaccctggc agcaccagta ccagcgggtc gagcattttc
    15481 agcaccgccg ttaacagcgc ccccttttgc ccttccgcca ggctttttga cgccagcgtg
    15541 cgttgcacga tgccctgatt tgtacaccag taaaaggtgt tcaccagaat caaaccggta
    15601 aatgccgcgc caatcggcaa gggatcggtc gggccaccga ttgagtttaa tttctcggcg
    15661 tgaacggtgg tgagttgctc aatgccctgc ataaagctgc ccttgcccat cgcgatcagg
    15721 ccaaataccg gcaccatcaa cccgccgata accagcccaa taccgttgat ggagtccgcc
    15781 actgccattg cgcgcagtcc gccgatcacc gcatacaaaa ttcccgccag accaagcaaa
    15841 attaccagta gccagatagc cgcaccgtga gaaatctgta gcgattcccc gacgtgaaac
    15901 aggctgttga gcgccaacgc gccggagtag agaacaatcg gcagaaagca gacgccggtg
    15961 gcaatgagga agcagaagtc gatgataata cgcgtcgttt tatcataacg ttcctccaga
    16021 aaatcgggga tggtggcaat gccgcgcttc agatagcgcg gtaaaaagat tagcgcgagg
    16081 aagatcagcg tcaccgctga agtcacttcc cagcccatca ccgacatgcc gcttttgtag
    16141 gcctgcccgg aaaggccgac cagttgttcc gtggaaaggt tggttagcat taacgaagcg
    16201 gcaataaccg gcgcttttag tgaacgtccg gcaagaaaat agccttgttg cgatccggta
    16261 tctgttttgc ggaccttcca ccaggtgatc accgccacca gcagcgtaaa accgacaaaa
    16321 ctcaagattt gtaacgaatt catctcatag cccttattta tgtttatgtc ccggacgcat
    16381 gaatataggg agaggtaaaa tgcgtcgggt caggcagtca gattcttaaa atacaaacgt
    16441 cgtatccctg aacggattgt tttctgttaa gttcaggttg tgagcataat tcgccggagg
    16501 gatattaaaa tgaatggaaa attgcaaagt tcagatgtaa aaaacgaaac tccgtacaat
    16561 attcccttat tgattaacga aaatgtgatc tccagcggaa tttctctgat ctcgctctgg
    16621 catacctacg ccgacgagca ttaccgggtg atctggccgc gggacaagaa gaaaccgctt
    16681 atcgccaact catgggttgc ggtgtatacc gtacaaggat gtgggaaaat tcttttaaag
    16741 aatggcgaac aaataacact gcatggcaac tgtattatat tcttaaagcc aatggatatt
    16801 cactcttatc actgtgaagg tttagtctgg gaacagtact ggatggaatt tacccccacc
    16861 agtatgatgg atattcccgt tggtcagcaa agcgttattt ataatggcga aatttataat
    16921 caggaactca ccgaagttgc tgagttaata acttcaccag aagcaataaa aaataatctg
    16981 gcagtcgctt ttctgacgaa aattatttat cagtggattt gtcttatgta cgcagatggt
    17041 aaaaaagatc cacaacggcg gcaaattgaa aaattaattg ccactttaca tgccagtctg
    17101 caacaacgct ggagcgtagc tgatatggct gccacgatcc cctgtagcga agcctggttg
    17161 cgtcgtctgt ttttacgcta taccggcaag acgccgaaag aatattacct cgatgcgcgt
    17221 ctggatctgg cgctatcgct attaaagcaa caaggaaact cggttggcga agtcgctgat
    17281 acgctcaact tcttcgactc ctttcatttc agcaaagcct ttaaacataa atttggttat
    17341 gcgccgtcag ccgtgctaaa gaatacggac cagcacccaa cggatgccag tccacacaat
    17401 taacgtaact ctggccagta cgctttgttg gcctccagca actcatccag aatagctttg
    17461 gcgaccgagg cgctcggtac cgttttcgac aacgtaatcg cctaccacag gtgctgatat
    17521 gaacgctgtt cccaggcgtc cacgaccagt ttttccaccg ccacttgctg actcattaac
    17581 cctttctgaa actgcgggat atcgccgacc accagtggtt caggccgtag tagcgcacgc
    17641 gcatctgttt gcgatcttgc agcccaacaa tttgcgccat ccggctttca ataccgattg
    17701 gcatgtcaca gatgttgagg attttcgcat tcgggcgcag acgacgcgtg gcttctgcga
    17761 caattgctgc cgggttggag tagttgagca tccaggcgtt tggtgaatat ttttccatat
    17821 aatccaccag ttccaggacg ccgccaatgg aacgcatgcc gtacgctatt ccgccgggtc
    17881 cgcaagtttc ctgaccaaca acaccgtggc gcagtgggat tttttcatcc agttcgcgca
    17941 tcgggtattt gccgacgcgg atgtgcgcca tgacaaaatc aacgtcgctg aacgccactt
    18001 caggatcggt ggtgtaacta aacgcaatgt ccggcgcttt ctctttaagg atgactttac
    18061 aggcttcggc aatcacttcc tgccgcgcgc catcgttatc ataaaatttc agtgcacgaa
    18121 gcgggaaacg ctcctgattc gccaggagca tcaacacgat gcctggcgta aaggtgcttc
    18181 caccgcctgc gacaaccact gagaatttgg tcattatact gcctccgtaa tggcaacatt
    18241 ttctgctgat tgatgagaat taattaagct atcgagctgt tcacgcagct gggatacatg
    18301 cagaccaatg atcacctgaa tggcatcgcc actacggaag acgccgtgcg ctcccagctt
    18361 tttaaagact tcgtcatcca gcgtttgtga catgtcatgc agtgcaatgc gtagacgcgt
    18421 cgcacaattg ttaatgctgg agatattgcc gaccccgccc agggcttgca ggataccggc
    18481 agcctgatcc agctcttttt ttggctctgc cgcggtggtt tggcctcgcg aggctttgta
    18541 ttcggctttt gagtagagtt tcacttccgc atcttcacgt cccggcgtgc acatgttgaa
    18601 ctgcagaatc agtgtgcgga aaaccacgaa gtacagcagg gtaaagcaca acccaatggc
    18661 gatttgggtc agcatcatat ccgcgtggtt gctgaacatc gggatccagt tttgcggtaa
    18721 aacctggtca atcagacctc cgcccatgtt gcccaccaca ccaaagagat acatcacggt
    18781 cgacattgag gccgcaagca cagcgtgtac cgcaaacagc aacggtgaaa tgaacaggaa
    18841 ggtaaattcc agcggttcgg taattcccac cagcatggcg gttaaggttg cgggaatcag
    18901 caagcccgcc acttttaccc gattttccgg tgcggcagtg aagtacatcg ctaaagaaat
    18961 gcccacggca ccaaagattt ttgagttacc gtgcagggca aaaccgcctt ccgggaacaa
    19021 cgatttcagc ggctcggcgc tcagactgaa ctcttgcaga tgctgcgccc agtacatctg
    19081 aatgccgcct tcaacagctg ccggaccaaa gataaacggt ccgtagatga agtggtgtaa
    19141 accggttggg atcagaatac gttcgaggaa ggtgtaaacc cacaccccaa gcgcacccgc
    19201 cgaacgcagg aacgcttgca gagattcaat ccccatttgt acttttggcc agccgagcaa
    19261 cgtcagccag gcacagggga tcatcaccag gaaggcgata atcaccacat aagacgtccc
    19321 ctggaaaatg ccgagaaaaa ccggcagttt tttatcgaac agacggttat gcagcgccgt
    19381 cacaatgccg gaaatgataa ttgcgccgat aatgctggta tcgagggttt taatcccggc
    19441 catcattgtc agaccgctac ctgccaccgc gtcctgagtg aaatcgacgc cgaagtagct
    19501 tccccaggtc attcccatcg cgttgatgaa atagttccag gtcaggaaac tcaccatcac
    19561 cgccagacaa gcacgcccct gcgcttgctt agcaaggcca atgggtaaac cgacagcaaa
    19621 aatcagcggc atattacgga acaccgtcca accgccctct tcaataatgt gtacgatttg
    19681 cgcgaataaa ctgttcggat cggtcagtga ttccccgaca aacatcgggt tttgcagcaa
    19741 gatggcaaga cccaccacaa tcccggcgaa gggaaacagc agcactggcg tgaacatcgc
    19801 gccgccaaag cgttgaattt gactgagcat ttccacatcc tcttttctca attctgtaag
    19861 taggaagcct gattggttat gcggcaggcc tggagcgagg ataggaagat gcgatcggca
    19921 gaacatgacg acccaatggg attcgtgatc gctttcatgc ttttcgttgg gcggtagaga
    19981 tctacgtttt cgcgaaaaag tagacatctg tgagcacagg tacacagcat cgaacacatc
    20041 tttaaaaaaa agatgttttt tcaatcgatt aagcagaact tgtgggcgca ttacccgtgc
    20101 ttgcaggcaa aaaagagatc tagagatgat ctacaaaagc attgcggagc ggttaagaat
    20161 tcgacctaat tccgcagatt tcacgctaaa tagccttctt cccggtgaaa aaaagttggc
    20221 ggaagaattt gcggtatcgc ggatgaccat ccgtaaagcc attgacctgc tggtagcgtg
    20281 gggattggtg gtccgccgcc acggcagcgg cacttacctg gtgcgcaaag atgtactgca
    20341 tcaaaccgcc agcctgaccg ggctggtgga ggtgttaaaa cggcagggaa aaacggtcac
    20401 cagccaggtg ctgatttttg aaatcatgcc tgcgcctccg gccattgcca gccagttacg
    20461 gattcaaatc aacgagcaga tctacttctc ccgtcgcgtt cgttttgtgg aagggaaacc
    20521 gctgatgctg gaagacagct atatgccggt aaaactgttc cgtaatcttt cgctgcaaca
    20581 tctggaaggg tcgaagtttg aatatattga acaagagtgc gggattttga ttggcggtaa
    20641 ttatgaaagc ctgacgccag tgctcgccga tagactgctg gcgcggcaaa tgaaggtagc
    20701 ggaacacacg ccactgctgc ggatcacctc gttgtcatat agcgagagcg gggagttttt
    20761 gaattattca gtgatgttca gaaatgccag cgaataccag gtggagtacc atttacggcg
    20821 actccacccg gaaaagagtt aaccgatact ccagaagagc accgccagta attggggggt
    20881 gataatgcgc aggaacatca ctaacggata gacagtggcg taagagagcg ccgccgcacc
    20941 gctggttgga tgaagattat tagcaaacgc cagcgccgga ggatcggtca tggaacctgc
    21001 cagcatcccg cacatggtca ggtaattcat tttggctaac atccgcgcca gaatgccaac
    21061 agtaatcagc ggaacggcgg tgatcagggc accataacca atccagctta gcccttcgcc
    21121 attgaccagg gtattcacaa aatctccacc agatttcaga ccaacgaccg agaggaacag
    21181 cacgatcccc agctcccgca gcgcgaggtt ggcacttggc ggcataaacc agtacagctt
    21241 gccgatactg ccgatacgcc cgaggatcaa cgccataatc agcggaccgc ccgccagccc
    21301 cagtttcaac gcggccggga acccaggcac aaagacggga atagaaccta acaatacgcc
    21361 aagcccgatg ccaataaaca ctggcagcat ctgaacctgt tgcagttttt gctgcgcatt
    21421 ccccagcaca ttggcaacgg catcaattgc ggacggacgc cccaccagat tgaggatatc
    21481 gccgaactgc aggctgatat cgccactggc gaccagttcg accccggcac ggttcaggcg
    21541 cgagataaca acgtcatagc gttctttaaa gtgcaggtcg cgaatacgtt ttccgagcac
    21601 gttttcattg gtgaccacca cacgctcgac gcgcaaatca gtgcctttcg tggacagcga
    21661 agtatcgacc tcctgaccaa tcaccagttg cgcattatgt aaatccgctg gctgccccac
    21721 cagatgcagc aaatcgccca gttggataat ggtatctggc gaaggaactt ttagggtttc
    21781 ttcgcgtttc agacgcgagc agataatttt gtcgccgttg agaatcggta catctttaat
    21841 ggctaaatca tgcaggttag ggttctcaac gcgaatattg atagtcttga tcagcgcgcc
    21901 gccattggtg cgtgaagact cgtgctgctg agcttctgtc tcgacattga cgcggaaaat
    21961 aacccgcaac atccacatgg tgaacaaaat cccgcaaatg ccgaatggat acgccatcgc
    22021 gtaactcatc cccatctgat cgaccatttc cattggtgta cccaagtcgc gcaaaatctg
    22081 ctgccctgcc cccagcgctg gcgtattggt aaccgcaccg gagaaaatcc ccagcactac
    22141 cggcagtgga atatcaaaca gtttatgcag gatggcggta accagaccac cgatgatgac
    22201 gatcagaaca gcaaacaggt tgaggcgtaa tccggagacg cgcaatgagg caaagaagcc
    22261 cggccctacc tgaatcccga tagtataaac gaacaggatc aggccaaatt cctgaataac
    22321 atgcagcata tcgctactta atgtcatccc cgcctgagaa acaaaatggc cgacgatgat
    22381 cccaccaaac agcacgccgc caatacctaa tcctatgccg cgaaatttga cattgccgat
    22441 aaacaaaccg acgactgcca ccaaagccag aatactgacc gttaatgcta tatcactcat
    22501 cgtccatttc tcttgagata acatttttaa ttgctacgga ttatggctga atcggtgaag
    22561 cagatttgcc ttatagcgca cattatcacg ggaaagaacg tgccgaaata tcttaaacag
    22621 tcgaaattta acgcattgtt gagtaagtaa gacaaaaaaa gccccaccag aatggcgggg
    22681 caaagagaat agctagttag ctatttaacg cgggacgttc gctgatagcg atacgctgcg
    22741 ctgcgatggg ttcaggctca ttacgaatta aatcaatatg cagtaaaccg tttacgaagg
    22801 ttgcgccaga gacttccata ttttcagcca gcgtaaagct caggctaaat ggctgattca
    22861 taagcccttg atgcagccat tttttctctt cttttggctg ctccggcgtg ccttttacgc
    22921 tcaggcgcgt accttccagt tgaatctcta aatcttcctg acggaaacct gccagcgcaa
    22981 gggtaatgcg gtagtggtta tcgtcgcttt tctcaatgtt gtacggcgga aagctctggc
    23041 tttcaccggc gttttgcagt gcgttggcca gtttgtcaaa accgatccat tgacgcatca
    23101 gtggggataa atcgaagtta cgcatagtca tttctccttc taagaagcga gtaagtacct
    23161 gcaaatccga agattcgcat atgctccctg acggcgagca tggagatgtc aggccgcgcc
    23221 aggcggcctt agggaattag ttgatttcga tacggcgcgg ttttttcgct tccggaatca
    23281 cgcgttcgag atcgatatac agcaaaccat ttaccaggtt agcaccacga acatgaatgt
    23341 tctcagctaa ctggaatttg cgttcaaagt tgcgttcagc gatgccctgg tacagatagg
    23401 tgcgctcttt ttgttcgtcg gcgtgagcac ctttcaccac cagcagatta tcctgggcgg
    23461 taatttccag ttcgctctca gcaaaaccag ccacagcgat agcaatgcgg taatggtttt
    23521 cgtctaccag ttcaacgtta tacggagggt agccgccatt actctggctc tggttgtttt
    23581 ctaagtggtt aaacaaacgg tcaaatccaa tagcagaacg gtaaagcggg gataaatcaa
    23641 agttacgcat aatcaatagc tcctgaaatc agcgagaatg taagaccttc cacaatggac
    23701 aggtcaggta gccagaacac ccatcaggcg cgtcctcatc ggctacgatg taaaaatggg
    23761 tctggaaatg aacttttcaa gccttatcag cggacttttt tttgcagttt atggtctatt
    23821 gcatagactg agggggcagc acgaaacgtt aaagtgcgat agccgccaca gtgcgacgta
    23881 atgatgatgt tattttcagc aagggatcgc tataactcat caggccaaca cccacgatca
    23941 acagatgaat ttattatgat aagaaatgtg ttgttagcgt tcatgatatg cagcggaatg
    24001 acattactcg gaggatgctc cagcgtgatg tcccataccg gagggaaaga aggaacgtat
    24061 cccggcaccc gcgccagcgc caccatgatt ggcgacgatg aaaccaactg gggcactaag
    24121 tcgctggcga ttctcgatat gccgtttacc gctgtgatgg acacgctttt actgccgtgg
    24181 gatgtgttcc gcaaagacag ctcggtgcgc tcacgcgttg aaaaaagcga agccaacgcg
    24241 caagccacca atgcggttat tccaccggcc agaatgcctg ataactgatt tagcgatcag
    24301 tttcggtgat ccacagctgg ccaccttcca tccacgccag ccattgacca tccggcgaga
    24361 agaccacggc gtccgcagaa ggtggattgg cgtggttttc ggttaaatac tcaacctcgc
    24421 cgctttgcgc atgggcacaa gcaattcgat tatccagcac aaagcccaac cattctcctg
    24481 acggatgcca gttaaatgca gactgaatat ccgttttgtt atgggttaac tggcgcggct
    24541 cgccgccctg tggcgagata agccacagtt gcacaatgcc gttatcatca cgcattaaaa
    24601 acgcgatttg cgtaccctgc ggattacagc gcacccagtg gcgcgggacg ttgactaacc
    24661 ccggataagc ccgatggtgg gtaaaggtta aacgtcgctg cacgacgccg cgcggtggcg
    24721 cgggcagggt ggtttccgtt ccacttaacg gcgcatcacc tgccgctttc cagccagctt
    24781 catcttgcgg taactcaacg ataaacagct ccggcacttt ctcgcccttt ggcgaaagtg
    24841 tgtcgccaat aaacgccagc gcgtgatttc ctacccatcc ttcttcataa gcacgattga
    24901 tttcatcgct gccaggctgc ggcgtgggcg tggttttact caccagcacg caccagtggc
    24961 taccgctgta ttcacgcgga tgctgctttt gtacgttgac cgggccaaac ggcgcagcaa
    25021 caccgacgtt tcgcaaatcc agcgccggat cgagttcatg cattacatgg tcgttatagg
    25081 taaagctcac cctttcaccg ttcgggctaa agacatgcac atggctgccg ccgcgcagcg
    25141 ctcctggcgt atacggcgcg gtaatatcca ttgcatcgag attgctcacc ttgccgcctt
    25201 cagcaatgac tccgcgtcga tgatggaagt cgtaatgcca tgtttcatca ggattttcag
    25261 ggccgtgaat aaagacatat ttctctgact tcggatgaac ggtcaccacg ccgacgtgtg
    25321 cgccctgtga cgcgcgatag ataacctcga cctcgcctgt atggatattc acacgctcaa
    25381 tggtctcgcc ggtaaacgac gccccagaag gacgcacgtc aaataccagc cactggctgt
    25441 cgggcgtcca ggtattggta ttggtgagca ggtgattacg gggagcaaag gttatctgtt
    25501 tcatggcgtg gtcctgatac aaaacggttc ccgccatcat acttcacaag gacttcactt
    25561 tcagacaata gtcttttgcc atcgtgatga atgtgagtga aaagatggaa catttcgacg
    25621 tggcgattat tggcctcggc ccggcaggat cggcgttggc gcgaaaatta gcaggcaaaa
    25681 tgcaggtgat cgcgctggat aaaaagcacc agtgtggtac tgaaggtttc agcaaacctt
    25741 gcggcggtct gctggcaccg gacgcgcagc gatcttttat tcgcgatgga ctgacgcttc
    25801 ctgtcgatgt gatcgccaac ccgcagattt tcagcgtcaa aactgtcgac gtcgccgcat
    25861 cgctcacgcg taactaccag cgaagctata tcaatattaa tcgccatgct ttcgacttgt
    25921 ggatgaaatc gctgatcccc gccagcgttg aggtttatca cgatagcctg tgccgaaaaa
    25981 tctggcgtga ggatgataaa tggcatgtca tttttcgtgc agacggctgg gagcaacata
    26041 ttactgcccg ctatctggtc ggtgccgatg gcgcaaactc gatggtgcgg cgacatctct
    26101 acccggatca tcaaatccgt aaatatgtcg ctatccagca gtggttcgca gagaaacatc
    26161 cggtgccgtt ctactcctgc atctttgata attcgataac taactgttat tcatggagta
    26221 tcagcaaaga cggttatttt atctttggcg gtgcttatcc aatgaaagac ggtcagacgc
    26281 gtttcacgac gctgaaagag aaaatgagcg cctttcagtt ccagtttggt aaggcggtga
    26341 aaagcgaaaa atgcacggtg ctgtttccct cacgctggca ggattttgtc tgcggtaagg
    26401 acaacgcctt tctgattggc gaagcggcag gatttatcag cgccagttcg ctggagggga
    26461 ttagctatgc gctggatagc gcagacattc tgcgttcggt gttactgaag cagccagaga
    26521 agctcaatac cgcttactgg cgcgccaccc gcaaactgcg tttaaaactc ttcggcaaga
    26581 tagtaaaaag ccgatgcctg accgcaccgg ctttaagaaa gtggattatg cgcagtggta
    26641 tggcgcatat tccacagttg aaagattagc caacgcgctt cacatcgccc accagcagga
    26701 tgtaagagag cgcgccaatc aacgcgacgg cggagatata aaccagtgca ggtgcgaaac
    26761 cgtaaccctg cgccaggtac cccaccacca gcggaacggt gatgccgccc agaccaccgg
    26821 cgaagttaaa cacgccgccg gttaaaccaa tcaggcgcat cggtgccaga gaagagacca
    26881 gcgaccaggt aatcgaagca aaaccgttgc cgaagaatgc cagcgccatc aggcacataa
    26941 tcatcatcgg atcgttggtg tagttagcgc ccataatgca ggtggagatc agcaagccgc
    27001 agataatcgg cgttttacgc gcaaagccca gtgaaaagcc cttacgtacc agcagatccg
    27061 cgacccagcc agagagcagg acgccgacaa acgccgcgag gaatggcacc gtggtcataa
    27121 agcctgcttt cagcgccgtg attccttttt cctgggttaa atagttcggg aaccaggtta
    27181 agaaaaacca cagtgtagaa gccaccgcaa attgcccaag ataaacgccg atcagtttac
    27241 gatggaacac cagtttccag tctttggctg ttaacggctg acgcgcctct ttcttcaccg
    27301 gcgcatcacc atccaccaga ccgccgccat cacgaatgta atccagttca gctttgctga
    27361 tacctttggt caggcgcggc ggctgataaa ccttaaacca aatcagcgac cagataatgc
    27421 cgataccacc agtgacaatg aacacccagt gccagctcaa catctcctga atccagatca
    27481 gcagcggagt caaaaacgcc agaccgacaa actgaccaga cgtataaaaa ccaacggcgg
    27541 aagcgcgttc atgttccggg aaccagctgg tcaccatccg gttattggtc gggaaagcgg
    27601 gcgcttcgaa aataccggtt atcgcacgca gaccaattaa tgacattaat cccgtggcaa
    27661 agccctggaa taaagtcgcc actgaccaac caaatatcgc aataaaataa gtcacgcgag
    27721 aacctacgcg atctaaaaac caaccgccgg ggatctggca tagcgtataa agccaggcga
    27781 aggccgaaaa tacatagccc atttccgctt tcgtaatgcc gaactcttcc tgaatatggg
    27841 cggaagccac ggccaggtta gcgcggtcaa cataacaaat gaccaccgta ataaagatca
    27901 tcaccagcgt cagataacgc cgacgccccg gctttgctgc attaacggga atatccatag
    27961 cgagctttct ccagattttg ggcatagcga agccgctcac catgccctgt aatttacaga
    28021 gggttatatt tttgtattgc tgttttagtg cccgatgagg ggcttacgtg gcaggaatta
    28081 ccactctgct acgctgttat cttcatgacg ccagagcgga ttacgccagt ccggggcatt
    28141 tttactgaac tcaataactt tagcttcgtc gatttccaca cctaagcccg gtttcgttaa
    28201 cggtttaaag aagccgccaa ccatactgaa gtcttctttg tttttcacaa agtcgagtaa
    28261 ctccgcgcct ttgttgtaat gaatgcccat actttgttcc tgaagtaccg cgttatagga
    28321 aacaaagtcg atatgcaggc aagccgccag tgcaatcggt ccgagcggac agtgcggcgc
    28381 aagggtcaca tcataggctt ctgccattcc agcaattttg tagcattcgg taataccgcc
    28441 tgcatgggag agatccggtt gcagaatcga aataccgcct gcctccagca cgcgtttaaa
    28501 atcgaaacgt gagaacatgc gctcacccgc cgccagtgga atatgcgttt gtgccgccag
    28561 tttcgggtag tattcggctt gttccgccag caccggctct tcaataaaca gcgggcgata
    28621 cggctccagt tctttaatca gcactttcgc cattggcgcg ctgacgcgac cgtggaaatc
    28681 aagaccaaac tcaatctgat tgccaaaagc ttcacgaatt tgtgccacgg tgttgactgc
    28741 cgcatctacc gcgcgggagt tatcaattag ccccagttct tcacaaccgt tcagtttgaa
    28801 ggtatcgaag ccgatttcgc gcagggtttt aatgccgtcg ataacatccg ccggacgatc
    28861 gccgccgacc caactgtagg ctttaatttt gtcgcgaacc aggccgccca tcagttgcca
    28921 gaccggcgca ttcagcactt tgcctttgat atcccataac gcctggtcga tcccggcaat
    28981 ggcgctcatc aggattggac cgccacgata aaatccggcg cgatacatca cttgccataa
    29041 gtcattgatg cgcgaaggat cctgaccaat caaatagtca cccagctcgt gaactgccgc
    29101 ttccaccgta cgggcgcggc cttcaatcac gggctcgccc caaccgacca cgccttcatc
    29161 ggtttcaatt ttcaggaaca tccagcgggg aggtaaacga tacgtagtaa ttttggtgat
    29221 tttcattgca ctgcctctcg atacgcctta acaaatgctg ctgcctgctg cgcggtgcgt
    29281 tctacggact gcccggcgcg atagagatcg ctgcctaagc ccgccccagc acaacctgcg
    29341 tctatccact gcgccaggtt ttctggcgtc acgccgccaa cggcaaagac tgcgatgtcc
    29401 gatggcaata ccgcttttaa cgctttgatg tattgcggac caaaagccga tgacggaaat
    29461 attttcagcg cctgcgcgcc cgcttcgagc gcggtaaagg cttcggtcgc cgtcgcgcag
    29521 ccggggcaga cggtcatgcc gtagcccacc gcacggcgga ttacttcact atggatattg
    29581 ggcgtaacga tgagctgaca gcccatcctg gcgagcgcat cgacctgttc aggtttcagt
    29641 accgtacctg cgccaatcaa cgccttgtcg ccatacgcat caacgatggc gggaatgctt
    29701 tgctcccatt gtggggaatt cagcgggatt tcaaccgcgt cgaacccggc gtcaatcacc
    29761 gcgccaacat gcgccagcgc ctcgtcgggc ttaatgccgc gcaaaatggc gatcagcgga
    29821 agtttagttt gccactgcat gagcgatgct ccttatacca gcctgaaatg ccgtgtcgcc
    29881 cgccaccgcc gtcacgtcgc aacccatcgc ctgaaaggct tgctggtagc gcgcggtcag
    29941 cgatgttccg gcgacaaggg tgatggcgtg ttgatgggtc acatagtcgc gcatactggc
    30001 aacctctgcg ccaatcaaca aaccagagag aaattcgctg acctgttcgc ggggaagtgt
    30061 tcccagcaca tgcgaggcgc gaacttcaaa aagctgcggc aatatggcgg gcgcattaag
    30121 gccgcgctca aggccagccg cgaaggcatc ggcagagttt tcctgcggcg gcaaacctgc
    30181 gccaatcaat gagtgattta acagtaaatg atgtaattca ccggtcatca cggtgcgaaa
    30241 atcgttgatt tgctggctat cggcctgcac ccatttgcaa tgcgtaccgg gcataacata
    30301 aagagaggaa ggagccagag ctcgcgcgcc gatcaattgt gtttcttcgc cgcgcatcac
    30361 attgtggtta tcgtcatgag agacgcataa tccgggaata atccagatat tgtcgccaac
    30421 agacgttaat tgttcgccaa tagacgaaaa acgggcagga acagataaat acggagcaac
    30481 tttccagccg acattgctgc cgaccattcc tgccatcacc actggcgtat tttcttcacg
    30541 ccagtcggtc gtgacttctg ctaacaccgc agccggagat tttccgttca ggcgcgtgac
    30601 gcctgcttct gattgcctgc tctccaggca gtggtcgccc tgataaagcc aggcgcgcag
    30661 attggtcgat ccccagtcaa ttgcgatgta gcgagctgtc atgtgatttc ctttaacctt
    30721 cgtgtcgagc tggcgatcat ggtaagcgcc gcctgctctg ccgcatcgcc gtcctgatgc
    30781 cgtattgcat cgaacagcgc cttatgttcc tggagcgttt gcggcatgtt ggcctcatcg
    30841 cccatccagg ttcgttcaaa aaccgcccgc tgcaacgaac tgatcgcaat gctaagttgc
    30901 tgtaacaccg ggttatgtac cgactgcagc accgcctcgt ggtagcgaat atccgcttcg
    30961 ttaaacgctt cgcggtcctg attgttggca atcatctcgt tcagcgccga ttcaatctgc
    31021 gccagatcgc tggaagtcgc gcgctctgct gcccaacggg caatcgccgg ttccaccaga
    31081 tttcgcactt cgctcatggc actgataagc cgtgggtcgt agtcgttttc cagcacccat
    31141 tgcagtacgt cagtgtcgag gtaattccac tggttacgcg gtgccacaaa cgccccgcga
    31201 taacgtttca tttcaatcag ccgcttcgcc atcagcgaac ggaacacctc acggatgatg
    31261 ttgcgcgagg ttgcaaactc ttcacagagc tccgcctcag ccggaagtgg cgagccaggt
    31321 acgtatttgc cgtgaacgat ctgtttaccc agcgtaatga caatgcgatc ggttttattg
    31381 agagtcatgg agagtccttg tgctctgtat gttcttctct actttacccc gatcgatgca
    31441 taacgcggca actttgtagt accagcgtga tgacgttcgc gtttgccgtg tgcgcaatgt
    31501 agtacaaact tatattgttg tactacaatt tagatcacaa aaagaacaat gcataaaaaa
    31561 atgacatgcg tcgggcagaa atctgaaaag ggatatcagg cgctaaacag gagggaaaga
    31621 agagtatgct ttcaacggct tagctactcg tttaaaggat taatcatgaa gttgaatttt
    31681 aagggatttt ttaaggctgc cggtttattc ccactggcgc tgatgctttc aggctgtatc
    31741 tcgtatgctc tggtttccca taccgcaaag ggtagttcag gaaagtatca atcgcagtca
    31801 gacaccatca ctgggctatc gcaggcaaaa gatagtaatg gaacaaaagg ctatgttttt
    31861 gtaggggaat cgctggatta ccttatcact gatggtgccg atgacatcgt taagatgctc
    31921 aatgatccag cacttaaccg gcacaatatt caggttgccg atgacgcaag atttgtttta
    31981 aatgcgggga aaaagaaatt taccggcaca atatcgcttt actactactg gaataacgaa
    32041 gaagaaaagg cactggcaac gcattatggt tttgcctgtg gtgttcaaca ctgtaccagg
    32101 tcactggaaa acctaaaagg cacaatccat gagaaaaata aaaacatgga ttactcaaag
    32161 gtgatggcgt tctatcatcc gtttaaagtg cgattttatg aatactattc acccagaggc
    32221 attccggatg gtgtttccgc agcattactg ccagtgactg ttacgctgga catcattact
    32281 gcaccgctgc aatttctggt tgtatatgca gtaaaccaat aatcagtaag cgggcaaacg
    32341 cgtttatgct gtttgcccgt ccactgatta attcagcaca tacttctcaa tagcaaacgc
    32401 cacgccatct tcaaggttgg atttggtgac aaagttcgcc acttctttca ccgaaggaat
    32461 agcgttatcc atcgccacac cgacgcctgc atattcaatc attgcgatat cgttttcctg
    32521 atcgccaatc gccatgattt cttccggttt aatacctaac acgtcggcca gtgatttcac
    32581 ccccgtacct ttgttaacgc gtttatcgag gatttcgagg aagtacggcg cacttttcag
    32641 cacggtatat ttctctttca cttcctgcgg aatacgcgcg atagcctggt cgaggatggc
    32701 gggttcatca atcatcatca ctttcaggaa ctgggtattg gggtccattt tctccgcttc
    32761 gcagaacacc agcggaatgg tggcaacgaa ggattcatgc accgtgtagt agctgatatc
    32821 acggttggcg gtgtacagcg tggtgcggtc cagggcgtgg aaatgagaac cgacttcgcg
    32881 agagagtttt tccaggaaac gatagtcgtc atagctgaga gcagtttgcg ccacggtgct
    32941 accatcagcg gccttctgta ccagcgcgcc gttataagta atgcagtagt cgcccggctg
    33001 ttccatatgc agctctttca ggtagttgtg cacacctgca tacgggcgac ccgtcgttag
    33061 cacgacattc acgccacggg cgcgagctgc ggcaatcgca tttttaacgg cgggtgaaat
    33121 ggtgtgatcg ggcagcagaa gggtgccatc catatcgata gcaatgagtt tgatagccat
    33181 gagttcccca ggtagattag ttcctgaccc atgctaacgc gattccgctc aaaaatcagt
    33241 acaacacccg agggaaaagg gggatgcaac gcgggttgca tgctccccat ttgcttagcg
    33301 gaagagtttc cctttcagca gttccatgcc tgcggaaagc agatcgttat tggcttgtgg
    33361 agacacttca ccttgcggtg agagcgcatc aataatcttc ggcaattgtt ctgccagtaa
    33421 actggaagct gtactggtat ccacgccaag tttttgtcca agatcggaca ccgcatttgt
    33481 gccgagcgcc gattccagtt gctcgccact aaccgattga ttgccctgtt gattactcag
    33541 ccaggttgag agaatggccc ctaagccgcc actttgcagt ttttccagca gcacctgaat
    33601 gccgccctgc tcctcaaccc aacttaaaat agcctgatat ttccccgcat cgcctttcag
    33661 aaaggcaccg acaacttcat caaaaagccc catgataatc acctgtaaag cgttacgtgt
    33721 tgacccaaaa agtatagatt tgcgggatga taattgcgga ttgcagaaat aaaaagggcg
    33781 gagatgatct ccgccctttt cttatagctt cttgccggat gcggcgtgaa cgccttatcc
    33841 ggcctacaaa atcatgaaaa ttcaatacat tgcaagattt tcgtaggcct gataagcgta
    33901 gcgcatcagg cacgctcgca tggttagcgc cattaaatat cgatattcgc cgctttcagg
    33961 gcgttctctt caataaacgc acggcgcggt tcaacggcgt cgcccatcag cgtggtgaac
    34021 aactggtcag cggcaatcgc atccttaacg gtaacgcgca gcatacgacg gctttccggg
    34081 tccatggtgg tttcccacag ctgttccggg ttcatctcgc ccagaccttt ataacgctgg
    34141 atggagaggc cgcgacggga ctctttcacc agccagtcca gcgcctgctc gaagctggct
    34201 accggctgac gacgctcgcc acgttcgata aacgcatctt cttccagcaa gccacgcagt
    34261 ttctcaccca gcgtgcagat acgacgatat tcgccaccgg tgataaactc gtgatccagc
    34321 ggatagtcag tatccacacc gtgggtacgc acgcgaacaa tcggctcgaa caggttttgt
    34381 tcggcattgg tgtggacatc aaacttccac tggctgccgt gctgttcttt gtcgttcagt
    34441 tcgctgacca gcgcgttcac ccagcgggta acggtctgct catcagagag gtcggcttcc
    34501 gtcagcgtcg gctgatagat aagctctttc agcattgctt tcggataacg acgctccatg
    34561 cggttgatca ttttctgcgt cgcgttgtac tcagacacca atttctctaa cgcttcgcca
    34621 gccaatgccg gtgcgctggc gttggtgtgc agcgttgcgc catccagcgc gatagagatc
    34681 tggtactgat ccatcgcttc gtcgtcttta atgtactgtt cctgcttgcc tttcttcact
    34741 ttgtacagcg gcggctgagc gatgtagacg tgaccgcgtt caacgatttc cggcatctga
    34801 cgatagaaga aggtcaacag cagcgtacga atgtgcgagc cgtcgacgtc cgcatcggtc
    34861 atgatgatga tgctgtgata acgcagtttg tccgggttgt actcgtcacg accgatacca
    34921 cagccaagcg cggtgataag cgtcgccact tcctgagaag agagcatctt atcgaagcgc
    34981 gctttctcga cgttgaggat tttacccttc agcggcagaa tcgcctggtt cttgcggtta
    35041 cgcccctgct tcgcagagcc gcccgcggag tccccttcca ccaggtacag ttcggaaagc
    35101 gccggatcgc gttcctggca gtctgccagt ttgcccggca ggcccgctaa gtcgagcgca
    35161 cctttacggc gggtcatttc acgcgcgcga cgcgccgctt cacgggcacg ggcagcatcg
    35221 ataattttgc caaccacgat tttcgcgtcg gttgggtttt ccagcaggta ttctgccagc
    35281 agttcgttca tctgctgttc aaccgctgat ttcacctcag aagaaaccag tttgtctttg
    35341 gtctgggagg agaatttcgg gtccggcact ttcacggaaa cgaccgcaat caggccttca
    35401 cgcgcatcgt caccggtggc gctaactttg gcttttttgc tgtagccttc tttgtccatg
    35461 taggcgttca gggtacgggt catcgccgca cggaagcctg ccaggtgagt accgccgtca
    35521 cgctgcggaa tgttgttggt aaagcagtag atgttttcct ggaagccatc gttccactgc
    35581 aacgccactt cgacgccaat accgtctttt tcagtggaga agtagaagat attcgggtgg
    35641 atcggcgttt tgttcttgtt cagatattca acgaacgcct tgatgccgcc ttcatagtgg
    35701 aagtggtctt ctttgccgtc gcgcttgtcg cgcagacgaa tggaaacgcc ggagttgagg
    35761 aacgacaact cacgcagacg tttcgccaga atttcatatt cgaactcggt cacattggtg
    35821 aaagtttcga ggctaggcca gaaacgcacc atggtgccgg ttttttcagt ctcgccggta
    35881 accgccagcg gggcctgcgg tacaccgtgt tcgtagatct gacggtgaat tttaccctcg
    35941 cgctggataa ccagctccag tttttgcgac agggcgttta ctaccgaaac accaacgccg
    36001 tgcagaccgc cggacacttt ataggagtta tcgtcaaatt taccgcctgc gtgcagaacg
    36061 gtcatgatca cttccgccgc cgatacgccc tcttccgggt gaataccggt cggaatgccg
    36121 cgcccgtcat cctgtacaga gacagagtta tcggcgtgaa tggtgacgat aatttcttta
    36181 cagtgacccg cgagcgcttc gtcgatagcg ttatctacca cctcgaatac catgtggtgc
    36241 agaccggtgc cgtcatccgt gtcgccgata tacatacccg ggcgcttacg caccgcatcc
    36301 agccctttca ggactttgat actggaggag tcataagaat tcgacatcaa cgtttctcgc
    36361 tcatttatac ttgggttaat ccgttatttt acccttttcc acggtaaaca tcttcgaatt
    36421 ttcgtccgac atgtctataa cgtgttcagc actgatcgcg ctgacaaaga cctgtgattg
    36481 cgtcgctttt aagcggctgg caagcagccc gcgacgctca tcatcaagct cagaggcaaa
    36541 atcatctatc aggtagagac accgccgccc gctttcacgg gtgaggaact ctccttgcgc
    36601 cagacgtaag gcgcacatca acagcttaag ctgcccacgc gataaggtat cttccaccgg
    36661 cgcaccgtcg gcgcgaatgc gtaagtccgc tttatgcggg ccatgcgcgg tgtaggttag
    36721 ctggcgatcg cgttcaaaat tacgttccag cacctcagca tattctgtct ctttctccca
    36781 gccgcgctgg aaagagaaag tcagagaaaa ctcagggaga aattgcttac aagtatcggc
    36841 catatcagcc gcgataccgg cgctatactc cgcgcgccag gtgctgattt gctccgccag
    36901 cgggataagt tctttatccc acgggcgtag ctgttcgtaa cgtgtcacct ggcgcagcgc
    36961 cgcattgcgc tgcttgagca atcgcttgag attgctccag gcggtgaaaa atccgggttc
    37021 gttgtgaaag catccccagt cgaggaatgc tcttctgtat ttggggccgc cgttgagtaa
    37081 agtaaaccct tctggcgtta tcaactgcat tggcatcagg tgcgccagtt ccgcgacctt
    37141 atgcccgtct gtaccgtcga tgcggacttt gctgtcgccc tgtttgtctt tggttaagcc
    37201 aatcgctgtc tcgcgctctt cgccctgtaa tcgcccgtgg agaacaaacg cctcctgctc
    37261 atggcgaatg acgcgaccaa tctgcaaact gcgaaacgcc cgaccatggc cgagcgtata
    37321 gatggcttcc agcacgctgg ttttgccact gccgttggca cctaccagaa agttaaagcc
    37381 gggagataaa gcgagatccg cggtttcaat gttgcggaaa tcgcggatca acaagcgggt
    37441 gagggacatt acagtctcat tggcatgaca acataggcag ccgactgtga tgcggcatct
    37501 tcaatctgca cgctggaaac cgaatcggtc agcatcatgc ggacgttttc acatttcagc
    37561 gcgttcagaa catccagcac gtagctgacg ttgaagccga tttccatctc cgcaccacta
    37621 taggtaacgt cgaggatctc ttccgcttct tcctgttccg ggttgttggc ggtgattttc
    37681 agctggtttt cgctgacata aagacgtacg ccgcggaatt tctcgttaga gagaatcgcc
    37741 gcgcgagcaa acgcctgctt gagcagatcg cagccagctt ccagatgttt gtccgggttc
    37801 ttcggcagaa cgcggcgata atccgggaag cgaccatcca ccagtttgga ggtgaagata
    37861 aagtcgccaa cgtgggcgcg aatgttgttg ctgccaatct gtacgcgcag cggattgtcg
    37921 ccgccgtcga gcatacgcat cagttcaatc acgcctttac gcggtacgat caccgaatgg
    37981 cttggcaaag attgaccaat tggcattgaa cagaccgcca gacggtggcc gtcggttgcc
    38041 acggtgcgca gttcttcacc ttcggtttca aacagcatac catttaagta atagcgaacg
    38101 tcctgatgcg ccatagaaaa ctgggtcgct tcaatcagac gcttcatcgt tgcctgcggc
    38161 agggtaaatt cgacttcact ctgccagtca tcgaggttcg ggaaatccgc cgctggcagg
    38221 gtagacagcg aaaaacggct acgcccggag cgtaccagca tccgttcacc ttccagctgc
    38281 acggcaattt ccgcgccttc aggcagacca cggcagatat caaagaattt gcgcgccgga
    38341 acggtcgtcg ctcctggctc gtgtggctga accagcgcaa cacgtgccac catttccatc
    38401 tcgagatcag taccggtcag cgacaacgta ccgtcagcaa cctgtaacag cagattaccg
    38461 agaatcggta gcgtaggacg accacctaac ggaccgctca cctgttgtag cggttttaat
    38521 aaatgctcac gttctacggt aaatttcata ggtttacgat gacaatgttc tgattaaatt
    38581 tgaaaaatct tctttgatat cgtggctctc ttcacgcaac tgctcgatct tacggcaggc
    38641 atgaagcacc gtcgtgtggt cacgaccacc aaacgcatcg ccaatctccg gcagactgtg
    38701 gttagtcagc tctttcgcca gcgccatcgc catctggcgc ggacgcgcca ccgagcggga
    38761 tcgacgcttg gaaaggagat ccgcaacttt gatcttgtag tactccgcca ccgtcttctg
    38821 aatattgtcg atggtgacca gtttttcctg caatgccagc aagtcgcgca gcgcctcacg
    38881 cacgaagtcg atggtgatcg cccttccggt aaagttggcg ttggcaatga cgcggttcag
    38941 cgccccttcc agctcacgta cgttagatcg tagacgcttg gcgataaaga acgccacttc
    39001 acccggcaaa cgaatgtcgt tttcgtcggc ctttttcatc aggatcgcca cacgggtttc
    39061 cagctctggc ggttcgatcg ccacagtcag tccccaaccg aagcgggatt tcaaacgatc
    39121 ctcaacgccg ttgatctctt tcggatagcg atccgaggtg agaatgatct gttgattacc
    39181 ttccagcagg gcgttgaagg tgtggaaaaa ctcttcctga gatcgttctt tattagcaaa
    39241 aaactgaata tcgtcgatca gcagtgcatc tacggaacgg tagtagcgtt taaactcttc
    39301 gatcgcgttg ttttgcaggg ctttaaccat gtcctgaaca aagcgctcgg agtgcatata
    39361 aaccactttg gcattcggct tgcgcgccat aatgccgtta cccaccgcat gcagcaggtg
    39421 agttttaccc agacccgtgc cgccataaag gaacaacggg ttataggcac cgccagggtt
    39481 atccgccacc tggcgagccg ccgcgcgcgc cagttggtta gatttacctt caacgaagtt
    39541 atcaaacgtg tgtttgacgt ttacgttaga acgataggtc ggttctgccg gggccgggac
    39601 gttatcccaa cctgagcgcg tagaaggcgc agcacgttgc ggctgcgttt gcgccacctg
    39661 tgcaggggcc gcgacgttgc tcgtcactgc cgcttgtggc gtttgcgtca ccggtttggt
    39721 gccgacttca aaacgcagct gtggggcatc cgctccgcag aaactggtta gcagtccatt
    39781 gatattatta aggtacttgt cccgtaccca atcgaggaca aaacggtttg gcgcgtacag
    39841 ggccagcgtg ttatcgctca gttccgcctg caatgggcgt atccacatac tgaattctgt
    39901 ggctggtaac tcatcctgca atcgggcaag acactgctgc caaagcgaaa gtgacacggc
    39961 ggactccact cgaacaaaag tcgataatga ctaaggctga aacattcatg attgttgacg
    40021 tacgtcgaaa agaccctgct tgagggtgac gcacgaaccg ctgtctgcgg ttatatgccc
    40081 gatcaagatc ctgcaaaacg atcgggaccg cggatcatag cctaaactgc gcaagagatc
    40141 ttctgtttct cacagatttt cccgatttat ccacaggact ttccagacct cgctaagtgt
    40201 aaacgatcct gccgcgaggc gggcacgatt tacgccgcat attggaaaat ttaatgacca
    40261 tagacaaaaa ttggcttaat cgatctaata aagatccagg acgatccttg cgctttaccc
    40321 atcagcccgt ataatcctcc acccggcgcg ccatgctggt tttcactggt gtgaggtcgt
    40381 acattttccc tgcgaaaagg tgcggaaaag cgcggtaaat aaggaaagag aattgactcc
    40441 ggagtgtaca attattacaa tccggcctct ttaatcaccc atggcttcgg tgtccatcgt
    40501 ttcatttttc ggcggatatc caataaagcc attgaattta ttcaagttta ggtagaaatc
    40561 gccatgaaac gcacttttca accgtctgta ctgaagcgca accgttctca cggcttccgt
    40621 gctcgtatgg ctactaaaaa tggtcgtcag gttctggcac gtcgtcgtgc taaaggccgc
    40681 gctcgtctga ccgtttctaa gtaataaagc taacccctga gtggttaagc tcgcatttcc
    40741 cagggagtta cgcttgttaa ctcccagtca attcacattc gtcttccagc agccacaacg
    40801 ggctggcacg ccgcaaatta ccattctcgg ccgcctgaat tcgctggggc atccccgtat
    40861 cggtcttaca gtcgccaaga aaaacgttcg acgcgcccat gaacgcaatc ggattaaacg
    40921 tctgacgcgt gaaagcttcc gtctgcgcca acatgaactc ccggctatgg atttcgtggt
    40981 ggtggcgaaa aaaggggttg ccgacctcga taaccgtgct ctctcggaag cgttggaaaa
    41041 attatggcgc cgccactgtc gcctggctcg cgggtcctga tagccctcat tcgggtctat
    41101 caacgcctga ttagtccgct actcgggccg cattgtcgtt tcactccaac ctgttcaagc
    41161 tacggaattg aggcattgcg caggtttgga gtgataaaag gcagttggtt gacggtgaaa
    41221 cgcgtattaa aatgccaccc tttacaccct ggtggtgacg atcccgtccc gcccggacca
    41281 tttgatacca gagaacacta acgatggatt cgcaacgcaa tcttttagtc atcgctttgc
    41341 tgttcgtgtc tttcatgatc tggcaagcct gggagcagga taaaaacccg caacctcagg
    41401 cccaacagac cacgcagaca acgaccaccg cagcgggtag cgccgccgac cagggcgtac
    41461 cggccagtgg ccaggggaaa ctgatctcgg ttaagaccga cgtgcttgat ctgaccatca
    41521 acacccgtgg tggtgatgtt gagcaagctc tgctgccggc ttacccgaaa gagctgaact
    41581 ctacccagcc gttccagctg ctggaaactt caccgcagtt tatttatcag gcacagagcg
    41641 gtctgaccgg tcgtgatggc ccggataacc cggctaacgg cccgcgtccg ctgtataacg
    41701 ttgaaaaaga cgcttatgtg ctggctgaag gtcaaaacga actgcaggtg ccgatgacgt
    41761 ataccgacgc ggcaggcaac acgtttacca aaacgtttgt cctgaaacgt ggtgattacg
    41821 ctgtcaacgt caactacaac gtgcagaacg ctggcgagaa accgctggaa atctccacct
    41881 ttggtcagtt gaagcaatcc atcactctgc caccgcatct cgataccgga agcagcaact
    41941 tcgcactgca caccttccgt ggcgcggcgt actccacgcc tgacgagaag tatgagaaat
    42001 acaagttcga taccattgcc gataacgaaa acctgaacat ctcttcgaaa ggtggttggg
    42061 tggcgatgct gcaacagtat ttcgcgacgg cgtggatccc gcataacgac ggtaccaaca
    42121 acttctatac cgctaatctg ggtaacggca tcgccgctat cggctataaa tctcagccgg
    42181 tactggttca gcctggtcag actggcgcga tgaacagcac cctgtgggtt ggcccggaaa
    42241 tccaggacaa aatggcagct gttgctccgc acctggatct gaccgttgat tacggttggt
    42301 tgtggttcat ctctcagccg ctgttcaaac tgctgaaatg gatccatagc tttgtgggta
    42361 actggggctt ctccattatc atcatcacct ttatcgttcg tggcatcatg tacccgctga
    42421 ccaaagcgca gtacacctcc atggcgaaga tgcgtatgct gcagccgaag attcaggcaa
    42481 tgcgtgagcg tctgggcgat gacaaacagc gtatcagcca ggaaatgatg gcgctgtaca
    42541 aagctgagaa ggttaacccg ctgggcggct gcttcccgct gctgatccag atgccaatct
    42601 tcctggcgtt gtactacatg ctgatgggtt ccgttgaact gcgtcaggca ccgtttgcac
    42661 tgtggatcca cgacctgtcg gcacaggacc cgtactacat cctgccgatc ctgatgggcg
    42721 taacgatgtt cttcattcag aagatgtcgc cgaccacagt gaccgacccg atgcagcaga
    42781 agatcatgac ctttatgccg gtcatcttca ccgtgttctt cctgtggttc ccgtcaggtc
    42841 tggtgctgta ctatatcgtc agcaacctgg taaccattat tcagcagcag ctgatttacc
    42901 gtggtctgga aaaacgtggc ctgcatagcc gcgagaagaa aaaatcctga ttcggtgagt
    42961 tttcgctaaa ataagggcgg tcagttgacc gccttttttc ttttcgtagg gcggataagc
    43021 accgcgcatc cgccacacaa agcaacagga acatcatgag cgataatgac actatcgtag
    43081 cccaggccac gcctccggga cgtggcggcg ttggcatcct gcgcatctcc ggcttcaaag
    43141 cccgtgaagt tgccgaaacc gtgctgggta aactgcctaa gccgcgctac gccgattatc
    43201 ttccgtttaa agacgccgac ggcagcgtgc tcgatcaggg gattgcgcta tggttccctg
    43261 gcccgaactc gttcaccggc gaagatgtgc tggaactgca aggtcatggc ggtccggtga
    43321 tcctcgacct gctgttaaaa cgcattctga ccattcccgg cctgcggatt gctcgccctg
    43381 gtgagttttc cgaacgcgcg tttcttaacg ataaacttga cttagcccag gccgaggcga
    43441 ttgccgatct tatcgacgcc agttcggaac aggcggcccg ttcggcactt aactcgctgc
    43501 aaggcgcatt ctccgcacgg gttaatcatc tggtagaagc cctcacccac ttgcgcattt
    43561 acgtcgaagc ggcaattgat ttccccgatg aagagatcga tttcctctcc gacggaaaaa
    43621 ttgaagccca gctcaatgac gttattgccg atcttgatgc agtgcgtgct gaagcacgtc
    43681 agggtagttt gttgcgcgaa gggatgaaag tggtgattgc cggacgtcct aacgccggta
    43741 aatcgagcct gttaaacgcg ctggcggggc gtgaagcggc aatcgtaacc gatatcgccg
    43801 gaactacgcg tgacgtgctg cgtgagcata tccacattga cggaatgccg ctgcatatca
    43861 tcgataccgc cgggctacgt gaagccagtg acgaagtaga acgtattggt atcgagcgcg
    43921 cgtggcagga aattgaacag gccgaccgcg tgctgtttat ggtcgatggc accacaacag
    43981 acgccgtgga tccggcagag atctggccgg aatttattgc ccgtctgcca gcgaaactgc
    44041 cgatcaccgt ggtgcgcaat aaagccgata tcaccggcga aacgctggga atgagtgaag
    44101 tgaacggtca cgcgttaatt cgtctctcgg caaggactgg tgaaggcgtg gacgtgctgc
    44161 gtaaccatct caaacagagc atgggctttg acaccaacat ggaaggcggc ttcctggcgc
    44221 gtcgtcgcca cctacaggcg ctggaacagg cagcggaaca tctacaacag ggcaaagcgc
    44281 aactgttggg agcctgggca ggtgaactgc tggcggaaga gttgcgtctg gcacagcaga
    44341 acttaagcga aatcaccggg gaatttactt cagacgacct gctggggcgg attttctcca
    44401 gcttctgtat tggtaagtaa ccgcgcttac gaagccgtat tctgactgtc agatgcggct
    44461 tcgcttcatt gttagcactc ctgttattcc tcaacccttt ttttaaacat taaaattctt
    44521 acgtaattta taatcttaaa aaaaagcatt taatattgct ccccgaacga ttgtgattcg
    44581 attcacattt aaacaatttt agaatagaca aaattcttga gtattatact gtagcctcgt
    44641 gtctttcgag gataagtgca ttatgaatat cttacatata tgtgtgacct caaaatggtt
    44701 caatatcgac aataagattg tcgatcacag cccttaattt gcccttctgt agccatcacc
    44761 agagccaaac cgattagatt caatgtgatc tatttgtttg ctatatctta attttgcctt
    44821 ttgcaaaggc catctctcgt ttatttacta gttttagtaa atgatggtga ttgcatatat
    44881 atctggcgaa ttaatggata ttgcagatgt aatattcaca gggatcactg taattaaaat
    44941 aaatgaagga ttatgtaatg gaaaacttta aacatctccc tgagccgttc cgcattcgtg
    45001 ttattgagcc agtaaaacgt accactcgcg cttatcgtga agaggcaatt attaaatccg
    45061 gtatgaaccc attcctgctg gatagcgaag atgtgtttat cgatttactg accgacagcg
    45121 gcaccggggc ggtgacgcag agtatgcagg ccgcgatgat gcgcggcgac gaagcctaca
    45181 gcggcagccg cagctactat gcgttagccg agtcagtgaa aaatatcttt ggttatcaat
    45241 acaccattcc gactcaccag ggccgtggcg cagagcaaat ttatattccg gtactgatta
    45301 aaaaacgcga gcaggaaaaa ggcctggatc gcagcaaaat ggtggcattc tctaactatt
    45361 tctttgatac cacgcagggc catagccaga ttaacggctg taccgtgcgt aacgtctata
    45421 tcaaagaagc cttcgatact ggcgtgcgtt acgactttaa aggtaacttt gaccttgagg
    45481 gattagaacg cggtattgaa gaagttggcc cgaataacgt gccgtatatc gttgcaacca
    45541 tcaccagtaa ctctgcaggt ggtcagccgg tttcactggc aaacttaaaa gcgatgtaca
    45601 gcatcgcgaa gaaatacgat attccggtgg taatggactc cgcacgcttt gctgaaaacg
    45661 cctatttcat caagcagcgt gaagcagaat acaaagactg gaccatcgag cagatcaccc
    45721 gcgaaaccta caaatatgcc gatatgctgg cgatgtccgc caagaaagat gcgatggtac
    45781 cgatgggcgg cttgctgtgc atgaaagacg acagcttctt tgatgtgtac accgagtgca
    45841 gaaccctttg cgtggtgcag gaagggttcc cgacatatgg cggcctggaa ggcggcgcga
    45901 tggagcgtct ggctgtaggt ctgtatgacg gcatgaatct cgactggctg gcttatcgta
    45961 tcgcgcaggt acagtatctg gtcgatggtc tggaagagat tggcgttgtc tgccagcagg
    46021 cgggcggtca cgcggcattc gttgatgccg gtaaactgct gccgcatatc ccggcagacc
    46081 agttcccggc acaggcgctg gcgtgcgagc tgtataaagt cgccggtatc cgtgcggtag
    46141 aaattggctc tttcctgtta ggccgcgatc cgaaaaccgg taaacaactg ccatgcccgg
    46201 ctgaactgct gcgtttaacc attccgcgcg caacatatac tcaaacacat atggacttca
    46261 ttattgaagc ctttaaacat gtgaaagaga acgcggcgaa tattaaagga ttaaccttta
    46321 cctacgaacc aaaagtattg cgtcacttca ccgcaaaact gaaagaagtt taattaatac
    46381 tacagagtgg ctataaggat gttagccact ctcttaccct acatcctcaa taacaaaaat
    46441 agccttcctc taaaggtggc atcatgactg atcaagctga aaaaaagcac tctgcatttt
    46501 ggggtgttat ggttatagca ggtacagtaa ttggtggagg tatgtttgct ttacctgttg
    46561 atcttgccgg tgcctggttt ttctggggtg cctttatcct tatcattgcc tggttttcaa
    46621 tgcttcattc cgggttattg ttattagaag caaatttaaa ttatcctgtc ggctccagtt
    46681 ttaacaccat caccaaagat ttaatcggta acacctggaa cattatcagc ggtattaccg
    46741 ttgccttcgt tctctatatc ctcacttatg cctatatctc tgctaatggt gcgatcatta
    46801 gtgaaacgat atcaatgaat ttgggctatc acgctaatcc acgtattgtc gggatctgca
    46861 cagccatttt cgttgccagc gtattgtgga taagttcgtt agccgccagt cgcattacct
    46921 cattgttcct cgggctgaag attatctcct ttgtgatcgt gtttggttct ttcttcttcc
    46981 aggtcgatta ctccatcctg cgcgatgcca ccagcaccac tgcgggaacg tcttacttcc
    47041 cgtatatctt tatggctttg ccggtgtgtc tggcgtcatt tggtttccac ggcaatattc
    47101 ccagcctgat tatttgctat ggaaaacgca aagataagtt aatcaaaagc gtggtatttg
    47161 gttcgctgct ggcgctggtg atttatctct tctggctcta ttgcacgatg gggaatattc
    47221 cgcgcgaaag ctttaaggcg ataatctcct caggcggcaa cgttgattcg ctggtgaaat
    47281 cgttcctcgg caccaaacag cacggcatta tcgagttttg cctgctggtg ttctctaact
    47341 tagctgttgc cagttcgttc tttggtgtca cgctggggtt gttcgattat ctggcggacc
    47401 tgtttaagat tgataactcc cacggcgggc gtttcaaaac cgtgctgtta accttcctgc
    47461 cacctgcgtt gttgtatctg atcttcccga acggctttat ttacgggatc ggcggtgccg
    47521 ggctgtgcgc caccatctgg gcggtcatta ttcccgcagt gcttgcaatc aaagctcgca
    47581 agaagtttcc caatcagatg ttcacggtct ggggcggcaa tcttattccg gcgattgtca
    47641 ttctctttgg tataaccgtg attttgtgct ggttcggcaa cgtctttaac gtgttaccta
    47701 aatttggcta aatccttcaa gaagccagcc attcgctggc ttcttgcctc tcaggaaatc
    47761 acttatgtcc aaatggcaac tcgcctgatt ctccttcacc acgtatgctt tgcgtcacct
    47821 tactatcagg acgctttagc ccatgtcccg ctttttaatt tgtagttttg ccctggtttt
    47881 actttatccc gccgggattg atatgtacct cgttggttta ccgcgcatcg ccgccgatct
    47941 caatgccagc gaagcgcagt tgcatattgc gttctccgtt tatctggcgg gcatggcagc
    48001 tgcgatgtta tttgccggta aagtggccga tcgttcaggg agaaagcctg tcgccatccc
    48061 cggcgcggcg ctatttatta tcgcttcggt gttctgttca ctggctgaaa ccagcacgtt
    48121 atttcttgca gggcgctttc tacaggggtt gggcgcaggc tgttgttacg tggtggcgtt
    48181 cgccattttg cgcgacacgc tggatgatcg acgtcgggct aaagtgctgt cgttactcaa
    48241 cggtattacc tgcatcattc cggtgttagc gccggtgctc ggacatctga ttatgcttaa
    48301 attcccgtgg cagagtctgt tctgggcgat ggcaatgatg ggcatcgcgg tactgatgtt
    48361 gtctttgttt attttaaaag aaacgcgccc tgcggccccc gcagcttcgg acaaaccacg
    48421 agaaaatagc gagtcgctgc ttaaccgttt tttcctcagc cgtgttgtta tcaccaccct
    48481 cagcgtttcg gtgatcctca ctttcgtcaa cacgtcaccg gtattgctga tggaaatcat
    48541 ggggtttgag cgcggtgaat acgccaccat tatggcgctg accgctggcg tcagcatgac
    48601 cgtttcattc tccacgccat ttgcgctggg aatttttaag ccacgtacgt tgatgatcac
    48661 ctcgcaggtg ttattcctgg cggcggggat cacccttgcc gtttcacctt cccatgcggt
    48721 ttctctgttt ggtatcacac tgatttgcgc cggtttctcg gtcggttttg gcgtagcgat
    48781 gagtcaggcg ttagggccgt tttcattacg cgcgggcgta gccagctcga ccttaggtat
    48841 tgcgcaggtt tgcggttcgt cactgtggat ttggctggcg gcggtggttg gtatcggcgc
    48901 atggaatatg ctgatcggga ttctgattgc ctgtagcata gtgagcctgt tgctgattat
    48961 gttcgtcgcg cctggacgcc ccgttgccgc tcatgaagaa atccatcacc acgcttgatc
    49021 tcaatttgct gctctgtctg caactgctga tgcaggagcg cagcgtgacc aaagcggcga
    49081 agcggatgaa cgtaacccct tcggcggtga gtaagtcgct ggcaaagtta agagcatggt
    49141 ttgacgaccc gctctttgtg aactcaccgc tggggctgtc gcccacaccg ctaatggtca
    49201 gcatggagca aaatctggcg gagtggatgc aaatgagcaa cctgctgctg gataaaccgc
    49261 accaccaaac gccgcgcggc ctgaagtttg agctggcggc ggaatcaccg ctgatgatga
    49321 tcatgcttaa tgcgctgtcg aaacagatct accaacgtta cccgcaggcg accatcaaat
    49381 tacgtaactg ggattacgat tccttagatg ccattactcg tggtgaagtg gatattggtt
    49441 tttccggtcg cgaaagccat cctcgctcgc gggagctgtt aagctcgcta ccgttagcca
    49501 ttgattatga agtgctgttt agtgatgtgc cctgcgtctg gttacgcaaa gatcatccgg
    49561 cactgcatga aacgtggagt ctggacactt ttttgcgtta tccgcatatc agcatttgct
    49621 gggaacagag cgatacctgg gcgctggaca atgtgttaca ggagctgggg cgcgaacgca
    49681 cgattgctat gagcctgccg gaattcgagc agtcactgtt tatggcagcg cagcccgaca
    49741 atctgctact ggcgaccgcg ccgcgctact gtcagtacta caatcaactc caccaactgc
    49801 cgttggttgc gcttcctctc ccgtttgacg aaagccagca aaaaaagctg gaagtccctt
    49861 ttaccctgct ttggcataaa cggaacagcc ataatccgaa gatcgtctgg ttacgggaaa
    49921 ccattaaaaa tctttacgcg tcgatggcat aaccgaatcg tatgaaacgc gaccctattt
    49981 cacaataaaa tgtaaaaatt ttgtaataaa cttgtctgaa tcgaactttt agccgcttta
    50041 gtctgtccat cattccagta aatgattact cttgtattca taatggacca ttaagcatgg
    50101 agcgaaaaat ggcgactcac tttgcccgag ggattttaac ggaaggacat ctgatttctg
    50161 ttcgtctccc ctcccagtgt catcaagaag cccgaaacat tccccttcat cgtcaaagcc
    50221 gttttctggc gtccagaggt ttactcgcag aactgatgtt catgctgtat ggcattggcg
    50281 aattgccgga aattgtcacc ctgccgaaag gtaaaccggt tttcagcgat aaaaatttgc
    50341 cttcgttttc tatttcctat gccgggaata tggttggcgt ggcgttaaca accgaaggtg
    50401 aatgtggcct cgatatggaa ctacagcgtg cgacgcgcgg gtttcatagc ccacacgcgc
    50461 ccgataacca caccttttca agcaatgaat cgctatggat cagtaaacaa aacgatccta
    50521 acgaagcgcg ggcgcagctc atcacgctgc gccgaagcgt gctaaaacta accggtgatg
    50581 ttttgaatga cgatccgcgc gatctgcagc tgctgcccat tgccggacgc ctgaaatgtg
    50641 ctcatgtaaa tcatgtagaa gcgttatgcg acgcggaaga cgtgctggtg tggtccgtgg
    50701 cggtcacgcc cacgattgaa aagctcagtg tctgggagtt agatggcaaa cacggctgga
    50761 aaagcctgcc ggatattcac agccgcgcca acaatcctac cagccggatg atgcgttttg
    50821 cccagctctc taccgtgaag gctttttcgc caaattgata gacaaccaca ggagtcatca
    50881 tgtctgaaaa attgaaggtg gttacgttac tggggagcct gcgcaaaggc tcatttaatg
    50941 gcatggttgc acgtaccctg ccgaaaattg ctccggcgag catggaagtc aatgcgttac
    51001 cttccattgc cgacattccc ttgtatgacg ctgacgtaca gcaggaagaa ggttttccag
    51061 caacggtaga agctctggcg gaacagatcc gtcaggctga cggtgtggtg attgtcacgc
    51121 cggaatataa ctactcggtt ccgggtgggc tgaaaaatgc catcgactgg ctttcccgcc
    51181 tgccggatca accgctggcc ggtaaaccgg tgttgattca gaccagctca atgggcgtga
    51241 ttggcggcgc gcgctgtcag tatcacctgc gccagattct ggttttcctc gatgcaatgg
    51301 tgatgaacaa gccggaattt atgggcggcg tgattcagaa caaagttgat ccgcaaaccg
    51361 gagaagtgat tgatcagggt acgctggacc acctgaccgg gcaattgacc gcatttggtg
    51421 agtttattca gcgagttaag atctaaataa aaaacccgcc agtaatcatg catggcgggt
    51481 ttttaatgcg ctatcgattt taatgagcgt cgataaatac aatcttcagg ataaacatca
    51541 gcgcaacgat gattacgcac gggctaaggt cacgcagacg cccggtacca atcttcatca
    51601 cgcagtagga gataaagccc agcgcaatac cttcggtaat cgaaaagctg aacggcatca
    51661 tcacggcggt aataaacgcc ggaacagatt cagtaagatc ctgccagttc acgcgagcaa
    51721 gacttgaggt catcaacacg ccaacgtaga tcaacgcgcc agctgcagcg tagcctggca
    51781 ccattcccgc cagcggcgac agaaagataa ccagcaggaa cagcagacca acaaccactg
    51841 ccgtcagacc ggtacgaccg ccaaccgata cgccggaaga ggactcaata taagccgtaa
    51901 cggaagaagt accgataaac gaaccggtca cggaagagat actgtcgaca tacagcgcct
    51961 gcttcatgcg cgggaattta cccttctcat ccgccaggcc tgctttatcg gtcacgccaa
    52021 tcagcgtacc ggaggagtca aacaggttga ccagcatgaa agagaaaatc acccctgcca
    52081 gcccgaggtt aaacgacccg gctaaatcta catggcccac gacagtcatt acgctcggcg
    52141 gcgcagaaac gatgccattg tagtggacat cacccagcat ccagcccagc agcgttgtca
    52201 ccacgatgga aaccagcacc gccgcgtgaa tgttgcgcga ggccagaata gcgatgatga
    52261 agaagccgag gatccccagc agcacgctgt gagaagtcag gttaccgatg ctgaccagcg
    52321 tttccgggtt agcgacaatc acacctgcat tcttcagtcc catcatacca atgaacagac
    52381 cgataccgct ggtgatgccc acacgcagac ttaccggaat gttagcaatc atccagtaac
    52441 gaacgcggaa aatcgtcagt aacagcagac caatcgcgcc ccagaagatt gcgcccatcc
    52501 cgacttgcca cggcaagccc atagcctgta caacgacaaa agcgaagaat gcattcaggc
    52561 ccatagcggg tgccagtgca actggcaggt tagcaaacag tcccatcata atactgccga
    52621 atgcagcaat cagacaggta gtaacgaaga cggcgctggt atccatgcca gcaacgccaa
    52681 gaatttgcgg gttaacaaaa acgatgtaaa ccatcgtcag gaaggtggta aaaccggcga
    52741 tcacttcggt ccgtgccgtc gtgccatgtt cgcgcagttt aaacacgcgt tccagcatcc
    52801 cctggccaga agcctgggtt gtgtgttgtt gactcattat ctatttccga acaaggaggg
    52861 aaaatccgtc gctatcgtat accaaaatga gacaatatgg acggctgaag ataacttttt
    52921 ttcattgtat ttgcttatac ggcaacgatt gcgtcatgcg aattggcatg acgaaaacgt
    52981 taaactaacg ataaaggaaa gacatgtccc agatagaagc ggtatttttc gactgcgacg
    53041 gtacgctggt cgacagtgaa gtcatttgct cccgcgcata tgtaacgatg tttcaggaat
    53101 ttggtattac gctcgatcct gaagaggtat tcaaacgttt caaaggtgta aaactgtacg
    53161 aaattatcga tattgtttcc cttgaacatg gtgttacgtt agcgaaaaca gaagctgaac
    53221 acgtttaccg tgcagaagtc gctcggctgt tcgattcaga actggaagcc atcgaagggg
    53281 ctggagcgct cctgtcagcg atcactgcgc caatgtgtgt ggtatctaac ggcccaaata
    53341 acaaaatgca gcattctatg ggcaagctga atatgttgca ctacttcccg gataaactgt
    53401 tcagcggcta cgatattcag cgctggaagc cagacccggc gttaatgttc catgcggcaa
    53461 aagcgatgaa tgtaaatgta gaaaactgca ttctggttga tgactcagtt gccggtgcgc
    53521 aatctggtat cgacgcaggt atggaagtgt tctacttctg cgccgacccg cacaataagc
    53581 cgatcgttca cccgaaagtc accaccttta cccatctttc gcagttacca gaactgtgga
    53641 aagcgcgtgg ttgggatatt acggcatagt tcttcacact cccttcactt accccgctta
    53701 aattggcgct caaaggtaag taaagggagt ttgatatgtc tgtttcacgt cgggtaatac
    53761 atcacggact ttattttgcg gttttaggac cgttaattgg tgttctgttt cttgtcctct
    53821 acatattctt cgcaaaagaa ccgctggttc ttttgcgaag aatacaggta cttccattgt
    53881 ttctcttatt gtcgattact acgggagtta ttcctgcatt gttaaccggc gtgatggtcg
    53941 cctgtctgcc ggaaaagatc gggtcacaga aaaattatcg ttgtctggct ggtggcatag
    54001 gtggcgtcgt tatcaccgag atctattgtg cagttattgt acatattaag ggcatggctt
    54061 cctcggagtt gtatgaaaac attctttctg gtgacagtct cgttgtccgc atcattcctg
    54121 cattgctggc aggtgtggtg atgagcagaa tcattacccg tctgcccgga ttggatattt
    54181 catgtcctga aacagactct ttaagttaag cgggatactt tatctttggg ctactcaaaa
    54241 gcagacagga tgttactatg actcaaaata tcaggccgtt accccaattc aaatatcatc
    54301 ccaagccact ggaaacaggc gcatttgaac aggataaaac cgtagagtgc gattgctgtg
    54361 aacaacagac gtcagtttat tactcgggtc ccttttattg cgttgatgaa gttgaacatc
    54421 tctgtccgtg gtgtattgcg gacggttctg ctgctgaaaa gtttgcaggt agttttcagg
    54481 atgatgccag catagaaggt gttgaatttg agtatgatga agaggacgaa tttgccggta
    54541 ttaagaacac atatcctgat gaaatgctga aagagttggt tgaacgcacg ccaggttatc
    54601 atggatggca acaggaattc tggcttgcgc attgtggcga tttctgtgct tttatcggct
    54661 atgtgggctg gaatgatata aaagatcgcc tcgatgaatt tgccaacctt gaagaagatt
    54721 gtgagaattt cggtattaga aattctgatc tagctaaatg cctgcaaaag cgtggtgatt
    54781 gtcagggtta tctcttccgc tgtctccact gcggcaagct gagactgtgg ggtgattttt
    54841 cgtagttatt taaataatga gaacaggccg gagcgtaatt catacatccg gccttatttc
    54901 ttcagcttaa ccgagcgcta attctgcggc agcggcttta tcagcgatta ccatcagcga
    54961 aggatgcagc tgcaacacgg aagctggaac atcttcggta accggacctt gcagcacatt
    55021 tttcagtgcc tgcgctttac ctgcgccact gacaattatc agcaagtttt tcgccgccat
    55081 gatgcttttc ggccccatcg tcacgtagct gtcaggcact aacgaaaagt cgccgcccaa
    55141 ctcaccatgc gccacaatat caaccatctc tccctggatc gggaactcca cggtttgctc
    55201 atggaaatgg gtcgtattcg gcaaattgcc acaaaaatga ccatctgcac ctaatcccag
    55261 caccaccaaa tccaggccgc cttcacgcgc cagtttctga tcatgctcgc ggtagttatc
    55321 aatagtgagc ttctggatat tctcttcttt gatccccgca ggggtgaaaa acagattacg
    55381 cagattggta atcgttacac cttctccctc tttgccgcga aagggaattt catcgaaatt
    55441 atagaaatag cagttatcat accagggctt acctttaacc agggtggtga ggtattcata
    55501 catgcctttg ggcgtgctac cggcggtaat tgccaggtta acacgacgcg tcttcgacat
    55561 ataaccaagc agatggtgtg ccgcgacacg gctcatttcc tggtaatctt cggtaatgat
    55621 taatttcatc ttagtgcctt attttattta aataatttct gtacgaaggc tgcataggca
    55681 ggccgccaga catccatttc atggttcagg ccgggatatt cctggtaatc aaacttaatt
    55741 tttttctgct caagctcagt tttcagcccg gcgatatcct tgccggttac gacatcttta
    55801 tcaccaacga ccacagtaaa attacgtagt tgctggttaa tagctgccgg atcgttcagc
    55861 cgggccgcga caccttcatc cggtacggtt gtcgtggtaa caccactgaa tgtggccagc
    55921 cagccaaagc tttccagatg attcattccg gaaaccagcg cctggtaccc gccttgtgaa
    55981 agccctgcca gcgcgcgacc atcggcatct ttacggacat taaaacgctt gctaatcagc
    56041 gggataatat cgttcatcag ttcgcgatct gccgctttag cattcagcgg ataaaagact
    56101 ttacgtcttt cctgaggcac gaaatcttcg ggaataatgc ccttcgcatc ggtttcggta
    56161 tccgggatca ccaccagcat cggtttaatt ttcccttcag ccagcaggtt atccatgatt
    56221 tgcgggatac gcccctgatc gatagcggaa cgtccggtat caccaaaacc gtgatagaaa
    56281 tagagcactg gcaaaggctc gcccatgccg gtgtatcctg gcggggtcca gacatacatc
    56341 tgacgttcag attgcaatgc gttggagtgg taagttatgg cgatcaaatc accatgcgca
    56401 acagaacgcg tatccagata actgcctggc accagaatca tactggagtt aacctggcgc
    56461 tgaggcttag tcattgcggt gcctgtatca atgctgcgta caccatcaac attgaaaaaa
    56521 tactcgtaca ggttgccttt caggataggt gtgcgccacg accagactcc tgcttcgtct
    56581 ttggtcatcg ggtgaatatt gtccggaacc ggaacaccca ccactacaga gacatttttt
    56641 gccccagggg caaagtagcg aaaggtcacg ctgttatcgg cattgacctg agtcacatac
    56701 tgtttcaccg ggatagtggg tgccgggctg gctggcatat ccgctgctat ggcccactga
    56761 gcagaaatac cgctggcaat agccagcgtt aaagcagcaa tttttatatt catcgtaata
    56821 ttcctttcat taagttccat taccaccaga tttcagcctg ggcaccgaca gcgaactggt
    56881 cgtctttatt gtcttcgaag gtgaagccat ccagttcgtt ttccagggct ttgatatacg
    56941 tggcgtagaa acgaatttcc ggacgcgagg tcaacatact ggtattgact ttaaaggtat
    57001 ggaagagcgt ggttttataa ccagactcat taaatttatt actattcgca tctttgtttt
    57061 gctgggtgaa atagcccagt tcaacacctg tctggttata ttggtcccaa atataggccg
    57121 ggcgaacaac cgcacgaata gattcgaaat cagagtgtgc gcctgtttcg tagctataaa
    57181 tatcgttacc gaaggagtaa acaatcgcgt tagccacaat aaaatgatcg ccgatatagg
    57241 cttcgccctg tgaagtcaga cgaactgctg ttccgccggt gtgatcacca taataacgac
    57301 cattaaaggt ggtaaatgga cttgcgccag cataacggcc aaagttactg gcgatagagt
    57361 tattcgcaac caggaaggag aattcgttga agccaccttt atcaaatttc tgcgttaaag
    57421 atgtgccgaa catccaggta tctttccagt cataataccc gttattatcc tgattatctt
    57481 tttccgatgc gctttcgttt gccgtgacat aacgaccact caccattaag gtcgctttat
    57541 cccataacgg gatatcttta tagcgtaaat caatagtatt ggtattaatc tgctgtttgt
    57601 tttgcaggct gcgatcgtaa tcatcgacat cttcgcgaac cagcgcgata tcaattttac
    57661 ccggaccgac tttccagttt tccagaccta cacccgctgc ggcatcagta cgctgcgttt
    57721 tccagtccag catctggatt tcaattttcg gcgcaccgtg tttacccacc cagaaatcag
    57781 cctctggcgc aaagggcagg aaacctttgg tggtaacgta catatcggag aactgcataa
    57841 agttctcgcc accggcgtta tcgccaaacc agccggtaga gtactgctga ccaacgttac
    57901 catccatcat cacaacggca tcaacccgtt tgccgttttc gttgtagaca cgttgtttta
    57961 actgcaaatc aaaccagccg gagtattcgt taccaaagcg gcccagagaa ccaatcgccc
    58021 atgatttagg tgaaccatga gaggcggtcc cccaaccaga acggtaatag ccgttgtaac
    58081 taaagccaat ttcatctttc acaaacttac tgaaatcttt cagagtcatg gaagaataaa
    58141 tcgagcctgt ctcactggca tttttctctt catttttgac cagtacagca tcaggttttg
    58201 cggcactact ggtcgggaac ggattggcgg catacccttg atcattcgta cttacgctac
    58261 gattcaccgt cgctggcgta tattttttct tctcttcatc tttatacttt ttgagttcgc
    58321 tttgcgtttc tcttaacgct ttttctaata actcaaggcg ttgttccacc gttaatgatt
    58381 cagccaatga ttgtgcagaa aaggctaacg gtgccattaa taagacggca gaggtaataa
    58441 gatttcgtct aaacataata tccctttatg gtgcaaagaa agaattaacg tatcgcatcc
    58501 agactattct gaatgcgacg ataattaagg tgctttattg gttatttttt taatgacagc
    58561 ccccgcgttt tgatcacttc tgcataccat ccgaagcttt tcttacgcgt tcttgtgagg
    58621 cttccttcgc cattatcatc acgatccaca taaataaagc cgtagcgctt agacatttgt
    58681 gaatgagatg cactgactaa atcaattggc ccccaactgg tgtaccccat aatatccaca
    58741 ccatcggcaa tcgcttcatt tacctgtacc aggtgatcgt ttaaataggc aattcgataa
    58801 tcgtcctgta tcgaaccatc cgcttcaacg ctgtcttttg cgcctaatcc gttctcgaca
    58861 ataaataacg gtttttgata acgatcccaa agcgtattta acagaacccg taatccaacc
    58921 ggatcaattt gccaccccca ctctgaactt ttcagatgcg gattggggat catattcagt
    58981 atgttgccct gcgcattttt attaatgctt tcgtcgtggg aaacacaacc agtcatgtaa
    59041 taactaaaag agatgaaatc gacggtatgt tttaaatctt ctgcgtcact ttcagtcatc
    59101 tcaatggtga tattgtggtc gcggaagaaa cgctgcatat agccgggata ctggccacgc
    59161 gcctgaacat caccaaagaa catccagcgc cggttctctt ccatggcctg caacatatcc
    59221 tgtggctggc aggtgagggg gtaaaccagc ccaccgagaa gcatattgcc gattttcgct
    59281 tcggggagca ggctatgaca ggctttaact gcccgcgcac tggcaaccag ttgatggtgg
    59341 atagcctgat aaacttccgc ctcgccactc tcttctgcca gccccacgcc cgtgaatggc
    59401 gcgtgtaacg acatgttgat ttcattaaac gtcagccata acgccacttt atgttggtag
    59461 cgagtaaaga ccgtgcgggc gtaatgctcg aagtgatcga tgaccgctcg attagcccaa
    59521 ccgccgtagt ttttcaccag cccatatggc atttcgtaat gggataacgt taccagcggc
    59581 ttgatccccg cctgcgccat ttcatcaaac agccgatcgt aaaacgctaa ccccgcttca
    59641 ttcggttcga cttcgtcgcc ctgagggaaa attcgcgccc aggcaatgga aatacgcaga
    59701 caggtgaagc ccatctcggc aaataacgcg atatcttccg ggtaacggtg ataaaaatcg
    59761 atggcgacat ctttgatatt ctctttcccc aggatgcgcg gttccatttt tcccattacg
    59821 ccatgaggct gtaaatctga ggtcgagatc cctttgccat cttcctgcca ggcaccttcc
    59881 acctgattgg cagctgttgc gccaccccaa agaaatgttt ctggaaatgc tttcataatt
    59941 aactcctttt atcgttagcg aatgatggat aacagcggtt cacctgcgct tatctgcgcc
    60001 gtgccgtggg gtaatacgtc cgtaaaatca tcgctattac tgattaatac cggcgtcgtc
    60061 agatcaaatc cggcctcgcg aatagcaggg atatcaaaag aaatcagccg atcgcctgta
    60121 ttgaccttgt cacccacgtt gacgtgagcg gaaaagaatt tgccgtccag ttttacggtg
    60181 tcgataccga catgaatcag gatctccaca ccatcatctg actcaatgcc aatggcgtgt
    60241 aatgtggcga acaacgaagc aattcgaccc gcaaccggag aacgcacttc accaaccgag
    60301 ggcagaatgg cgataccttt acccaacagg ccactggcaa acgtggtatc agcgacgtga
    60361 atgagcggca caatctctcc cgtcatcggt gaacagatac cgccctgctc aggtggtgta
    60421 ataacctctg gtgttttctc ttgcggggca ccctgcgctg gctgacgttt agcggtgatg
    60481 aaatgaagca tcaccgtacc gacaaatgcg caaccgatgg caatgacacc gccaataacg
    60541 ctggcccaga cggtgaaatc aattcccgtt gacgggatgg tttgcatgaa ggtgaaaata
    60601 cttggcaaac caaaggagta gactttcgtt tgcgcgtagc caatgatggt ggcccccaaa
    60661 gccccactga tacaggcgat aacaaagggg tacttacgcg gcaggttgac gccatatacc
    60721 gctggttcgg tgataccaaa cagactcgtc aacgccgctg atcccgccac cacttttttc
    60781 tgcgcatcgc gttcgcagag gaagacgccg agcgccgccc cgacctgcgc cataatggcg
    60841 ggcattaaca gcgggatcat ggtgtcgtag cccagcacgg tgaagttatt gatacacagc
    60901 ggcaccaggc cccagtgcag tccgaacatg acgaagattt gccagaagcc gcccattacc
    60961 gcgcccgcaa atgcaggaac cgcctgataa agccagagat aaccggcggc aatcagttcg
    61021 cttatccagg ttgatagcgg ccccaccagc agaaaggtga cgggtgtgat aaccatcaga
    61081 catagcaatg gtgtgaagaa atttttgatt gccgacggta accacgcatt aagtcggcgt
    61141 tccagaatgc tgcacaacca ggcagaaaaa ataatgggaa taaccgatga cgagtaattc
    61201 aacaatgtga ccggaatacc caggaaatcc agccccagcg catccgcttt ttgcccgttc
    61261 tcgaaagcag tcagaattaa tggatgcact aacgctccac caatcaccat ggcagtaaat
    61321 ggattaccgc cgaagcgttt ccccgcggtg tatcccagga ttatcgggaa gaaccaaaac
    61381 aaggcatcac tggcgctgaa taaaattaaa taagtaccac tttgttcggt cgtccactga
    61441 aaagtgagcg ccagagccag catacctttc aggatcccgg ttgccgccat caaaccgatc
    61501 agaggcgtaa aaatacctga aataacataa acaaagcggt ttagcagatt acctttatca
    61561 tcattttccg gtgcctgttg cgctttttcg tcaaggcctg ccacactgtt aaccgccagg
    61621 aagacatcgg ccacatggtt acctatgacc acctgaaact ggccaccgct ttccaccacc
    61681 ataataatac cgggggtctt tttcagtacc tctgcttgcg ctttgctttc atcctttaac
    61741 ttaaaacgta atcgcgttgc gcaatgcatc agactcacaa tgttatctgc gcccccgact
    61801 cctgcgacta tttttctggc taactccgtc ataacttgcc ctctaccgct ttgcggcaaa
    61861 actcaaaaaa aaaacctgaa aaaaacggcc tgacgtgaat caagcaattt tttcaggttt
    61921 tgcccgctta gtgcggtaac aatcctttac tcagtaataa tatttcagtg ttctttgtgc
    61981 acgcgctcta tatttatggc taaaaacata atctctgcgg gtgagatttt acgttgatac
    62041 tgcaaaccaa taaaaatggc gatccgctcc gcacattgcc atgcttgcgg gtaattttgt
    62101 tttactgctt gttgtaatga ctcatcacta tcgttaattg aagcatgtcc aagaatacgc
    62161 caggataaaa acttcagatg cgtaaccagt cgctgataac tcaagctttc ttcctggtaa
    62221 ttaaggctga actgaaattt tattaattgc agcatttcgc gcattaattg cgtgacacct
    62281 gcaacatcct ccatatttcc gctcatttgg gcactgacca gatgcatggc aataaagccc
    62341 acttcatctt tcggtaactg cacgcccaac cgtttatcaa taatggttaa cgcttcttcc
    62401 cctagctgga actctttcgg gtaaagccgc tggatatccc acagcagcgg gttgggcagt
    62461 aacacgtttt gctgaaagcg tttaatcgca aactggcaat ggtcagttag cgagatataa
    62521 atactgtcct gtaattttcc caagcgctcc tgcgctaaag agataatacg atcacaggtt
    62581 gccatcacct caagaggaat atgacttaag agttcgctta atcgcccgtt cagttcatga
    62641 ctgctcaagg catactcttt ttctattcca cttgagttaa ttctttcgcc agcgcgtttt
    62701 tgaaagccaa ttcctcgccc catgacgact ttttcccgct gttgatcatc aataaccacc
    62761 acaacattat tgttgagaat tttggtgatt tgcatgttca tagcaaggac cttttttata
    62821 aacaaaaaaa cccgacttca ccagtattct ctggttatgt caggttttgc ctgcgaatgc
    62881 agtaacaatc cagtcattta ttaatggttt ttataacgaa catccaggtt cggaaattaa
    62941 tttaattgcg tgcagagaaa atagcaatgc gctattgata aaaatatgac catgctcgca
    63001 gttattaact ttgtgtaatt ttaggaattt ataaagttat atataacaaa tcccaataat
    63061 taagttattg ggatttgtct ggtgaattat ttgtcgctat cttttcccgc cagcagttta
    63121 tccagctcat caccgcctac atgacggaaa tcctgcccct tcacgtagta gaagataaac
    63181 tcacaaatat tctggcagcg gtcgccaata cgttcgatag aacgcgcgca gaacagcgca
    63241 gtaagtacgc tcggaatggt acgcgaatct tccatcatgt aggtcatcag ttgacgaaca
    63301 ataccttcgt attcctgatc gactttttta tcttcacgat aaatacgtac cgcttcgtca
    63361 atgtccatcc gcgcgaacgc gtccagcacg tcgtgcagca tctggatggt atgacggccc
    63421 agcgactcca gacttaccag caacggctga tgctgctggg agaatttctc cagcgcagta
    63481 cggcagattt tgtccgccac gtcgccaata cgctccagct cggcaatggt tttactgatc
    63541 accataacca gtcgcaggtc gctcgccgtc ggctgacgtt tggcgataat gcgcacgcag
    63601 gcttcatcga tcgccacttc catcatgttg acgttcttgt cgccttcgat cacgcgcttc
    63661 gccagatcgc tgtcctggtt atgcatcgcg gtgattgcat cagaaagctg ctgctccacc
    63721 atgccgccca tggtcatcac ctgcgtgcgg atactttcca gttcggcgtt gaactggccg
    63781 gaaatatgtt tattaagatt gagactgtcc ataacgcact cctgaatcaa ccgtaacgac
    63841 cggtgatgta gtcttctgtt tgtttcttcg ctggcttggt gaacagatcg tccgtgttgc
    63901 tgaactcaat caattcgccc aggtacataa acgccgtgtg gtcggaacaa cgcgcagcct
    63961 gctgcatgtt gtgggtgacg atcaccacgg tgtaatcctg cttcagttcg gtgatcagct
    64021 cttcaatacg cccggtagag atagggtcga gcgccgaaca cggttcgtcg agtagcagca
    64081 cttccgggcg aatggcgata ccacgcgcaa tacacagacg ctgttgctga ccaccagaga
    64141 gagagtaacc gctctggtgc aatttatctt tggtttcgtt ccacaatgcg gctttggtca
    64201 atgcccactg cacgcgctcg tccatgtcgg cacgggagag cttctcaaac agacgaacgc
    64261 caaaagcgat gttgtcgtag atggacatcg gaaacggcgt cggtttctgg aacaccatgc
    64321 ccactttcgc acgcagcagg gcgatatcct gagagttggt caggatgttg tcgccatcaa
    64381 gcagaatttc accttctgcg cgctgctccg gatacagttc aaacattttg ttgaaggtac
    64441 gcagcagcgt cgatttaccg cagccggacg gcccgataaa cgccgttacc tggtttttag
    64501 cgatatccag gttgatgttt ttcagggcat ggaatttgcc gtagtagaag ttcaaattac
    64561 gaacctgaat tttactcggg gcagtttcaa ccatactcat tgcaatcttt tcctcatcgt
    64621 acctgatgct tttcgctaat caggcttacc gtaggccggg taaggcgttc acgccgcatc
    64681 cggcaaagtg ttaaccgtgt ttattcttcg caaaaacaac gcgcgccaga atgttcagca
    64741 gcaggacgca cagggtaatg atcaataccc cggcccaggc cagttgttgc cattccgcaa
    64801 acgggctcat cgcaaactta aagatcgtca ccggcaggtt ggcgatcggc tgcatcatgt
    64861 ccgtgctcca gaactggttg gagagcgcgg taaacagcag cggcgcggtt tcaccggcaa
    64921 tacgggcaat cgccagcagg ataccggtca taatcccgga caccgacgct ttcagcgtaa
    64981 tcgcggagat catcttccac ttcggtgtac ccagcgcata agccgcttca cgcaggctgt
    65041 ccggcaccag tttcagcatg ttctcggtgg tgcggataac gatcggcacc tgcaacagcg
    65101 ccagcgcaat tacgcccgcc cagccggaga agtgctccat ctgtgccacc acgatggtgt
    65161 agacaaacag accaaccaca atcgacggcg cggagagcag aatgtcgtta atgaagcgaa
    65221 tcacttctgc cagccaggat ttacgaccat attccgccag ataaatcccc gccataatgc
    65281 ccagcggcgt accgaatacc gtggcccaca aaattaacag cccgctaccc gccagggcgt
    65341 tcgccagacc accaccttcc gtattgggcg gcggcgtcat ttcagtgaac agcgccagcg
    65401 acataccgtc gataccgcga gtgatggtgg acattaaaat ccagatcagc cagaacaggc
    65461 cgaaggccat cgtcgccatc gagagcgtca gcgcaatacg gtttttgagg cggcgacgcg
    65521 cctgcatttt gcggcgagat tcagccagcg ccgcagtggt ttgcatttca accatagcca
    65581 ttagcgtgcc ccctcattct tagccaggcg cataatcata aacttcgatg cggcgaggac
    65641 gatgaaggta atcacaaaca ggatcaggcc cagttccatc agtgcggcaa cgtgcagacc
    65701 ggattccgct tccgcaaatt cgttcgccag cgcagaggtg atactgttgc ccggcatata
    65761 cagcgaggcg ctgtcgagct ggtaggtgtt accgatgata aaggtcaccg ccatggtttc
    65821 accgagcgcg cggcccagcc ccagcatgat gccgccgata acaccatttt tggtgaacgg
    65881 aagaacgata cgccagataa cttcccaggt ggtgcagcca ataccgtagg ccgactcttt
    65941 catcatcacc ggggtttgtt cgaacacatc acgcattacc gccgcaatgt acggaataat
    66001 catgatggcg aggatcacgc ctgccgcgag gataccgata ccaaatgcgg ggccagagaa
    66061 cagcgcgcca acaatcggga tattcgacat gatattgccg accggctcct gaaagtaaac
    66121 ggcgaacagc ggcgcaaaga taaacaggcc ccacatgccg taaacgatac ttggaatggc
    66181 tgccagcagc tcaatggcga tacccagcgg gcgtttcagc cagccaggcg caagctcagt
    66241 caggaacagg gcgataccga aactcaccgg gacggcgatc agcagcgcga taaacgaagt
    66301 caccaacgta ccgtagatcg gcaccagcgc cccgtagata tcgttcggtg catcccactc
    66361 tttggtccat aggaaagcca gaccaaattt ctgaatgctc ggccaggagg agatgatcag
    66421 agagacaata atgccaccca acatcaatag cacaatcagc gccgccagtt ttaccagcac
    66481 gctgaaaatt atgtcgccct ttttacccgg tgggttaaaa gcaggcttgg ttgcagccat
    66541 aagttactct tcagttaaac gcgtttacaa agtcattttg taatgccgga tgcggcgtaa
    66601 aacaccgtac ccggcctgga gttttattag tacagcggct taccgctact gtctttaata
    66661 ttggtcttcc acgcagcgcg aacctgttca actacactat ccggcaggct ggcgtaatcc
    66721 aggtcgttcg cctgtttagc cccggttttg tacgcccagt cgaagaattt cagcacttct
    66781 gtgccttgtt ctggtttctt ctgatctttg tggatcagaa tgaacgtggt agaggtaata
    66841 ggccatgcat cttcgccttt ctggttggtc agatcctgag cgaaggtttt gctccagtct
    66901 gcaccttttg ctgcattagc gaagttttct tcggtcggac taaccggttt accatcagcg
    66961 gagatcagtt tggtgtacgc caggttgttc tgcttcgcgt aagcatattc aacataacca
    67021 attgcacccg gcagacgctg aacgaacgcg gcgataccgt cgttaccttt accgcccaga
    67081 ccgatcggcc attttacggt agagccagta ccaacgttgt ttttccactc ttcgttcact
    67141 ttcgccaggt agctggtgaa gacgaaggaa gtcccggagc catctgcgcg gcgtactaca
    67201 gcaatgtttt gtgaaggcag tttcagaccc ggattcagtt tggcgatggc ttcatcatcc
    67261 cacttcttga ttttgcccag gtagatgtcg ccgagggttt taccatccag caccagttcg
    67321 ccagacttca gccctggaat gttaaccgcc agcaccacgc cgccaatcac ggtcgggaac
    67381 tggaacagac cttcctgagc cagtttttcg tcagacagcg gcgcgtcaga ggcaccaaaa
    67441 tcaacggtat tagcgataat ctgttttacg ccaccggaag aaccgatacc ctggtagtta
    67501 actttattac cggtttcttt ctggtaagtg tcagcccatt tggcatacac cggcgcaggg
    67561 aaggttgcac ctgcacctgt caggcttgct tctgcaaaca cagagaaagc gctcatcgat
    67621 aaggtcgcgg cgacaacagt tgcgacggtg gtacgcataa ctttcataat gtctcctggg
    67681 aggattcata aagcattgtt tgttggctac gagaagcaaa ataggacaaa caggtgacag
    67741 ttatatgtaa ggaatatgac atttttatga cagaaaggaa ttaccaaata acttactgat
    67801 tttaaatgtg aagatgaaaa tcccacatgt catctttttg tcatgatgca tttaaaagtg
    67861 ataaagaaat gggtgagatg acaacgatgt gatgaacaat cgttgtcaga cctattagcg
    67921 cacaatctca acagttatcg tcgctgttgc agtaaattgc cccggggctg ggcgagctcc
    67981 agtaaggctc gcaggagcag ctgaaaaagt tacactacct gatttttcac catacactgt
    68041 tgatttaccc atgtctgttg gcaactcacc accgttcact ttgatttcct tattattttt
    68101 gtcgtaaatt tttatcccca cgttagagtt tgaagttacg accattgaat tgtccgtatt
    68161 gggttcggta gcataaaaag acgtactgat atcatcggta ttcagtatcc ctaggttttc
    68221 acactcaata gtgaccgttt tagtaatttt atgtgttgtt accgcacttc caacaacaga
    68281 tgaaaaatct gcagctggta tttctttaaa atcaaaataa actggctgta aattatttat
    68341 ttcgcaactt tgcggagctg taatatcgcc ccgaatataa acatcagcca tcggctgtga
    68401 gtaatccacc ggggtgtcac ggctaattgt tccatacaat gtcactatct ttgtttcagg
    68461 aataatcaat tttcctgcaa atgtcttctt gatataaaaa tatactatcg ctttagcccc
    68521 actagatata cttaaaggtt caatgcggtt gcaattgtac tttgtattac cagacgggtt
    68581 gttagcatga tattcaaaag gtgcattcac atacccaacc ccaagaatat ctatagcaat
    68641 cccaacatca aggttcggat taagatcata ataattaata ttagagtgaa ccccagcata
    68701 agttaatgcc tggtttattt ttgacgtata atagatatag gggaattctc ctacgttacc
    68761 agtatcacaa tgacagcgtc caggatatga ggatccatta gttacagtgg tgttaaatgt
    68821 cgttccaacc ttattttcgt tcgcggtaat aagatatccc gagaaatcaa gcgtactgtg
    68881 aaatacgcca ttatcagggg tacagatacc tttaggagct gcaagtgcta cactacttaa
    68941 cgagagcata aacacagcaa agcaccactg tttgatatac ttgttcataa aaatcctttt
    69001 aatgacatgt cacaccctgt tcaattaatc cgctctcact ttctggtgtc gaaagtgaat
    69061 aattgaagat gcattttgtt gaggcgtttt ctccccagga taacgtaacc tgtccttttt
    69121 ctggtagacc agaaagataa agttcgccac ggtcaccgac aattcctgaa tctgccaaac
    69181 ttgcatcttg tatcgcagca atggctccaa aaggtaccgg tttaccattt gcatttaaca
    69241 ctctgaagag aacgcgataa cccacatgag tcacaaactc cgctttaacc actgcaccac
    69301 gcgtgggaac catcgtaacg acggtattat caagttctat attcgacttc gagaaatagt
    69361 tatcgctgat tgctacccga ttttcatcat aagggtttag ctgtgtcttg acggtatagc
    69421 cgcgccagtc cgtcgcagca ccgcgcatat tatctatttc taacccggcg gccccaggtg
    69481 ctttaacaag agcaatagtt tcacctaact cctgacttaa cgttacccct tcgctgtgtg
    69541 ccagaattcc ccctctgata ccataattca gcctctggga attttggctg tagttatagc
    69601 tgccgttcac cgtgccataa gagccatcat aggtcaggga catattaccg ccaacctcat
    69661 catcatgact gttataggac tgcattaaat tccatgaaag gttatttctt tccagcgccg
    69721 taccgctgag tccgagttga tgtgagctac cggaattatc gttttgcgaa acagaatagg
    69781 tggcgtaact attaccccac cagcccgata aaggaacaga taaagagagc atgtagatct
    69841 gctcactggt gttatcactg gcatggttgt tccccgtccc ctgtctggca gtattggaat
    69901 aactccagga gacgttccac gagagacgct tccagctact gctgtagcca aattgcatta
    69961 atcgttcgac atcatcggta tgccagtatt gttgccggct taatgtcagg tataaattgc
    70021 cataatctct ccccaacgac tgcgttagcg ttccctccac gcgactacgt cggttacctg
    70081 tttcccaaaa gtcttcaggg ctgttgcgcc gcgatgccca ctcattgagg gtatagtagc
    70141 cctcggtgga gtaacgatag ccaaccaggc gcaagctagt gtctgtgtcg tcaaatcgtt
    70201 ttgaatagag aaagcgatat gactgacccg tttctgtatc atcatggcta aaattcgcac
    70261 gagcatgtgt cacatcgaaa gaaattgcgc ccaaagcgcc aagatcttta ccgacaccca
    70321 gagctgcaga accataattt tcagcagcct gcatacctcc atataaagtg atcccgtggg
    70381 aaaaaccatg aagtatttgc gcctgaagga cgtccggtgt aaatccgtct tcatcgcgca
    70441 attcacccac gctgacatca acatctgtct gaccttcacg tttaagaatc gccaatgaag
    70501 cataaggttg ggtgaatcgt tgttcgctac cgtcttcttc tttgatggtt acgtccaaat
    70561 cgccacctgt actggcggtg ttgagatcgg tgatctcaaa agcaccttgt ggaacggcag
    70621 actgataaat cacgtagcca ttctgccgaa tgatgacggt ggcgttggac tttgcaatcc
    70681 cacgaaccac aggagcaaag ccattctggc tggcgggtag catatcgtta tcagtataca
    70741 aacgcgcgcc gcgaatttgc gtactgtcga aaatatcgct cgccgtccac gtatcaccaa
    70801 tcataatctg gcttcttaac gcggcaatat cgcgctgtat ccaggtacta atggagttgt
    70861 attccgcacg gccatcgctg gtgtttaaag tactgtaatt acgtagccgc cacggtccaa
    70921 tattcagtcc attgcgcaga ttgagataac tggtatctgt ctcttgtgaa ccataatctg
    70981 aacgtagtgt atgtgaacca gaaaaatcgt agttgatggt gagcgcgtta atcccctcat
    71041 cccaacgcgt ggggctgatg taatcataag ccatcgcgtc catgtagatt tggggaatgg
    71101 atatattcag ctgcaattta tcactgtcaa actgccagga tgagccttca atacgtggag
    71161 caggatcgag gcaaaccgtc tcatccacat cattgaacga tttgagcgca tcaactttta
    71221 tgccaaagga ggacagtgtt tttttaatgg caataaagca agcgtgcaga tcagcatctg
    71281 gattatcctg aacaaatgtc acatcctgac gttcaaacat tttaccgttg ataaaaatat
    71341 caacgctata aacacctggg ggcatcaggt ttgcccgatt aaactggcga atatcgactc
    71401 cggattgatg gtcgatttcc agaagcgccg gattgaatac actgacctcg gccatcaggt
    71461 tttgcgagaa aatcagacac gtccctgccg tgaggccaac cactattctg gtcgtcatca
    71521 taatacgcct atccttatcc cttgcttaca gcgtcatttt tatcgggtca ctgactgcgc
    71581 cgtaatcatt aataaatcgc caggtcattt ggcctcccgc agaattagca ggtaaaggta
    71641 acgtgagcgt ttcaaaagga cggatcatat tgaggggcac atcatggcca tcaacgatga
    71701 catccatcac actgatatga attgggttgg gattctctgc tatcagattt cgcccttccc
    71761 ggcgccattt cactttcggc gcagcatctt cgggaataag acggatattt gctggtcgcc
    71821 agaacagctt gatacgcgtt tttactgcta tttccagcga gttcgtcgct ttgttgctcg
    71881 ctgatggcgg aatattttta atattcagcc agaagactga ctcgcgatca tcaggcaatg
    71941 acacaccttt ggtgtgaata atgcgtaata ttttttcttg cgaaggttct aatcgtgata
    72001 ctggcggaat aacggtaaac ggcgctttgc ttttatcctc tttattaaat ggatctaccc
    72061 aagcctggat gagataaggt attttttctt ctttgctaaa tacagaaata gatttatcag
    72121 cattattact taagtaaacc acccgcgtac caccaatgac aacgcctgca taagttaatg
    72181 ctggttggcc taaaaaaacg ctgagtataa tcgcggtaaa taacttacat gaaactgttg
    72241 ttttaaagag cctgattaaa caactttttc tggacataac gactccctgg ccaaagccaa
    72301 ctgaataaaa aggcccttga taaattattt cttatattcg acagtaaatt gtgaatcagc
    72361 gttcgccgta ccagccgtta cagtcgcatt ggttgccaca tagcgtgcaa taaacttcat
    72421 ttcagccttg ccttcagcat cgatagcggt ataagcagag tcgttataaa gtttaacctg
    72481 cgaattatcg gactggttaa aaatacgaat accaacgccg gttgccgcaa tcgcttgtcc
    72541 tgtaccggta aaatcaccag ggttgcctgc tgtaagcggc gtacccactt tcaggtcgcc
    72601 gtcgccgcca ggcgcttctg taccatcaaa acgaacggct gcttgagtgt acgttgccgg
    72661 gcaattttca agtttaatac tgaacttctg tggcgacgcc agactaccta cacctgaaaa
    72721 tgcattttta ctgactttac ccagtgggac aatttgatcg gctgtatcgg aggtaacttc
    72781 acaagtagtg tcgacaattt caccgacgaa gtgaacaaca ccatcttctg ccagtgcatg
    72841 cccggccagc attagagatg ccagagtgaa tgcaccgcct ataatattac gcttcataaa
    72901 aactccattg atataatata tcctttacca tgaatcatca cttatattaa ta
//
LOCUS       DAJOWB010000009        72006 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJOWB010000009 DAJOWB010000000
VERSION     DAJOWB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.009"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            49..789
                     /locus_tag="OHO85_000688"
     CDS             49..789
                     /locus_tag="OHO85_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCQ2670111.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            824..3424
                     /gene="htrE"
                     /locus_tag="OHO85_000689"
     CDS             824..3424
                     /gene="htrE"
                     /locus_tag="OHO85_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HCQ2670112.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RLSYSVNTGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIE"
     gene            3441..4010
                     /locus_tag="OHO85_000690"
     CDS             3441..4010
                     /locus_tag="OHO85_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2670113.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            4025..4627
                     /gene="yadL"
                     /locus_tag="OHO85_000691"
     CDS             4025..4627
                     /gene="yadL"
                     /locus_tag="OHO85_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HCQ2670114.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            4654..5250
                     /locus_tag="OHO85_000692"
     CDS             4654..5250
                     /locus_tag="OHO85_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2670115.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            5303..6577
                     /locus_tag="OHO85_000693"
     CDS             5303..6577
                     /locus_tag="OHO85_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HCQ2670116.1"
                     /translation="MKKFFRHFLFLILCLSCYTASAGTDDNVGYIVGNSYGVGPSDQK
                     WRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTGVKLQWSQLDTASGGGFLYCNRSD
                     STSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYYTLLISNMWSAYGTVTNVSSPGIY
                     IGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAVGGIYQTLTIEFYTDTNFDPTVTQ
                     QIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFDLLNVKLTNPTCFTAMLSGTSVTG
                     STVKMGEYSAEQIRNGATPVPFDISHQNCVRVTNIETKLVSTKVGTENGQLLGNTLTG
                     NDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYDPRGKDDTTGGVYPDQDTGITY
                     PLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            6690..7484
                     /gene="panB"
                     /locus_tag="OHO85_000694"
     CDS             6690..7484
                     /gene="panB"
                     /locus_tag="OHO85_000694"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCQ2670117.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITDALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7496..8347
                     /gene="panC"
                     /locus_tag="OHO85_000695"
     CDS             7496..8347
                     /gene="panC"
                     /locus_tag="OHO85_000695"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCQ2670118.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(8421..9323)
                     /gene="rpnC"
                     /locus_tag="OHO85_000696"
     CDS             complement(8421..9323)
                     /gene="rpnC"
                     /locus_tag="OHO85_000696"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCQ2670119.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMTNLSLAEIDKVINLI"
     gene            9597..9977
                     /gene="panD"
                     /locus_tag="OHO85_000697"
     CDS             9597..9977
                     /gene="panD"
                     /locus_tag="OHO85_000697"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCQ2670120.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(9981..11210)
                     /locus_tag="OHO85_000698"
     CDS             complement(9981..11210)
                     /locus_tag="OHO85_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCQ2670121.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLMSNQPQG"
     gene            complement(11274..11714)
                     /locus_tag="OHO85_000699"
     CDS             complement(11274..11714)
                     /locus_tag="OHO85_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCQ2670122.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(11819..12589)
                     /locus_tag="OHO85_000700"
     CDS             complement(11819..12589)
                     /locus_tag="OHO85_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2670123.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(12586..13512)
                     /locus_tag="OHO85_000701"
     CDS             complement(12586..13512)
                     /locus_tag="OHO85_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2670124.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            13621..14283
                     /gene="can"
                     /locus_tag="OHO85_000702"
     CDS             13621..14283
                     /gene="can"
                     /locus_tag="OHO85_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCQ2670125.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(14324..14860)
                     /gene="hpt"
                     /locus_tag="OHO85_000703"
     CDS             complement(14324..14860)
                     /gene="hpt"
                     /locus_tag="OHO85_000703"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCQ2670126.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15066..17456
                     /gene="gcd"
                     /locus_tag="OHO85_000704"
     CDS             15066..17456
                     /gene="gcd"
                     /locus_tag="OHO85_000704"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCQ2670127.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWLSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKNPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(17658..19208)
                     /gene="cueO"
                     /locus_tag="OHO85_000705"
     CDS             complement(17658..19208)
                     /gene="cueO"
                     /locus_tag="OHO85_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCQ2670128.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQHGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            19374..19721
                     /locus_tag="OHO85_000706"
     CDS             19374..19721
                     /locus_tag="OHO85_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCQ2670129.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            19827..20693
                     /gene="speE"
                     /locus_tag="OHO85_000707"
     CDS             19827..20693
                     /gene="speE"
                     /locus_tag="OHO85_000707"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCQ2670130.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLTHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            20709..21503
                     /gene="speD"
                     /locus_tag="OHO85_000708"
     CDS             20709..21503
                     /gene="speD"
                     /locus_tag="OHO85_000708"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCQ2670131.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(21541..21903)
                     /locus_tag="OHO85_000709"
     CDS             complement(21541..21903)
                     /locus_tag="OHO85_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCQ2670132.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22078..24675)
                     /gene="acnB"
                     /locus_tag="OHO85_000710"
     CDS             complement(22078..24675)
                     /gene="acnB"
                     /locus_tag="OHO85_000710"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCQ2670133.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25030..26844
                     /locus_tag="OHO85_000711"
     CDS             25030..26844
                     /locus_tag="OHO85_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCQ2670134.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSSQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHRDGNGWQHNGDNINIDVNNFNR
                     ITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSATQLPAPTRDSQRQ
                     AAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFSRRQPLTQQQKDAA
                     RQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQAVREKMQTNPQIQ
                     QRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQ
                     ESRPQRLNDSNHTVRLNNEQRSAVRERLSERGARRQER"
     gene            complement(26915..28339)
                     /gene="lpdA"
                     /locus_tag="OHO85_000712"
     CDS             complement(26915..28339)
                     /gene="lpdA"
                     /locus_tag="OHO85_000712"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCQ2670135.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(28547..30439)
                     /gene="aceF"
                     /locus_tag="OHO85_000713"
     CDS             complement(28547..30439)
                     /gene="aceF"
                     /locus_tag="OHO85_000713"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCQ2670136.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(30454..33117)
                     /gene="aceE"
                     /locus_tag="OHO85_000714"
     CDS             complement(30454..33117)
                     /gene="aceE"
                     /locus_tag="OHO85_000714"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCQ2670137.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(33278..34042)
                     /gene="pdhR"
                     /locus_tag="OHO85_000715"
     CDS             complement(33278..34042)
                     /gene="pdhR"
                     /locus_tag="OHO85_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCQ2670138.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            34582..35955
                     /gene="aroP"
                     /locus_tag="OHO85_000716"
     CDS             34582..35955
                     /gene="aroP"
                     /locus_tag="OHO85_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCQ2670139.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(35998..36852)
                     /gene="ampE"
                     /locus_tag="OHO85_000717"
     CDS             complement(35998..36852)
                     /gene="ampE"
                     /locus_tag="OHO85_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCQ2670140.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(36849..37400)
                     /gene="ampD"
                     /locus_tag="OHO85_000718"
     CDS             complement(36849..37400)
                     /gene="ampD"
                     /locus_tag="OHO85_000718"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCQ2670141.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            37488..38381
                     /gene="nadC"
                     /locus_tag="OHO85_000719"
     CDS             37488..38381
                     /gene="nadC"
                     /locus_tag="OHO85_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCQ2670142.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            38584..39024
                     /gene="ppdD"
                     /locus_tag="OHO85_000720"
     CDS             38584..39024
                     /gene="ppdD"
                     /locus_tag="OHO85_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCQ2670143.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTHYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            39034..40419
                     /gene="gspE"
                     /locus_tag="OHO85_000721"
     CDS             39034..40419
                     /gene="gspE"
                     /locus_tag="OHO85_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HCQ2670144.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLARTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMCTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            40409..41611
                     /gene="hofC"
                     /locus_tag="OHO85_000722"
     CDS             40409..41611
                     /gene="hofC"
                     /locus_tag="OHO85_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCQ2670145.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAILHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRENTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(41646..42689)
                     /gene="guaC"
                     /locus_tag="OHO85_000723"
     CDS             complement(41646..42689)
                     /gene="guaC"
                     /locus_tag="OHO85_000723"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCQ2670146.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGYEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(42845..42889)
                     /locus_tag="OHO85_000724"
     CDS             complement(42845..42889)
                     /locus_tag="OHO85_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670147.1"
                     /translation="MIIKTLKMSARKRN"
     gene            42914..43534
                     /gene="coaE"
                     /locus_tag="OHO85_000725"
     CDS             42914..43534
                     /gene="coaE"
                     /locus_tag="OHO85_000725"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCQ2670148.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            43534..44277
                     /gene="zapD"
                     /locus_tag="OHO85_000726"
     CDS             43534..44277
                     /gene="zapD"
                     /locus_tag="OHO85_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCQ2670149.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            44287..44484
                     /gene="yacG"
                     /locus_tag="OHO85_000727"
     CDS             44287..44484
                     /gene="yacG"
                     /locus_tag="OHO85_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCQ2670150.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(44700..45089)
                     /gene="mutT"
                     /locus_tag="OHO85_000728"
     CDS             complement(44700..45089)
                     /gene="mutT"
                     /locus_tag="OHO85_000728"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCQ2670151.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVERWEGKPWGKEGQPG
                     EWMALVDLNADDFPPANEPVIAKLKRL"
     gene            complement(45149..47854)
                     /gene="secA"
                     /locus_tag="OHO85_000729"
     CDS             complement(45149..47854)
                     /gene="secA"
                     /locus_tag="OHO85_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCQ2670152.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(47916..48428)
                     /gene="secM"
                     /locus_tag="OHO85_000730"
     CDS             complement(47916..48428)
                     /gene="secM"
                     /locus_tag="OHO85_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCQ2670153.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(48659..49576)
                     /gene="lpxC"
                     /locus_tag="OHO85_000731"
     CDS             complement(48659..49576)
                     /gene="lpxC"
                     /locus_tag="OHO85_000731"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCQ2670154.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(49677..50828)
                     /gene="ftsZ"
                     /locus_tag="OHO85_000732"
     CDS             complement(49677..50828)
                     /gene="ftsZ"
                     /locus_tag="OHO85_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCQ2670155.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(50889..52151)
                     /gene="ftsA"
                     /locus_tag="OHO85_000733"
     CDS             complement(50889..52151)
                     /gene="ftsA"
                     /locus_tag="OHO85_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCQ2670156.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(52148..52978)
                     /gene="ftsQ"
                     /locus_tag="OHO85_000734"
     CDS             complement(52148..52978)
                     /gene="ftsQ"
                     /locus_tag="OHO85_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCQ2670157.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPERTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(52980..53900)
                     /gene="ddlB"
                     /locus_tag="OHO85_000735"
     CDS             complement(52980..53900)
                     /gene="ddlB"
                     /locus_tag="OHO85_000735"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCQ2670158.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(53893..55368)
                     /gene="murC"
                     /locus_tag="OHO85_000736"
     CDS             complement(53893..55368)
                     /gene="murC"
                     /locus_tag="OHO85_000736"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCQ2670159.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPARVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(55422..56489)
                     /gene="murG"
                     /locus_tag="OHO85_000737"
     CDS             complement(55422..56489)
                     /gene="murG"
                     /locus_tag="OHO85_000737"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCQ2670160.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(56486..57730)
                     /gene="ftsW"
                     /locus_tag="OHO85_000738"
     CDS             complement(56486..57730)
                     /gene="ftsW"
                     /locus_tag="OHO85_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCQ2670161.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(57730..59046)
                     /gene="murD"
                     /locus_tag="OHO85_000739"
     CDS             complement(57730..59046)
                     /gene="murD"
                     /locus_tag="OHO85_000739"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCQ2670162.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALHPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(59049..60131)
                     /gene="mraY"
                     /locus_tag="OHO85_000740"
     CDS             complement(59049..60131)
                     /gene="mraY"
                     /locus_tag="OHO85_000740"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCQ2670163.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(60125..61483)
                     /gene="murF"
                     /locus_tag="OHO85_000741"
     CDS             complement(60125..61483)
                     /gene="murF"
                     /locus_tag="OHO85_000741"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCQ2670164.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(61480..62967)
                     /gene="murE"
                     /locus_tag="OHO85_000742"
     CDS             complement(61480..62967)
                     /gene="murE"
                     /locus_tag="OHO85_000742"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCQ2670165.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(62954..64720)
                     /gene="ftsI"
                     /locus_tag="OHO85_000743"
     CDS             complement(62954..64720)
                     /gene="ftsI"
                     /locus_tag="OHO85_000743"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCQ2670166.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(64736..65101)
                     /gene="ftsL"
                     /locus_tag="OHO85_000744"
     CDS             complement(64736..65101)
                     /gene="ftsL"
                     /locus_tag="OHO85_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCQ2670167.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(65098..66039)
                     /gene="rsmH"
                     /locus_tag="OHO85_000745"
     CDS             complement(65098..66039)
                     /gene="rsmH"
                     /locus_tag="OHO85_000745"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCQ2670168.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(66041..66499)
                     /gene="mraZ"
                     /locus_tag="OHO85_000746"
     CDS             complement(66041..66499)
                     /gene="mraZ"
                     /locus_tag="OHO85_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCQ2670169.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(67101..68105)
                     /gene="cra"
                     /locus_tag="OHO85_000747"
     CDS             complement(67101..68105)
                     /gene="cra"
                     /locus_tag="OHO85_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCQ2670170.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(68187..68273)
                     /locus_tag="OHO85_000748"
     CDS             complement(68187..68273)
                     /locus_tag="OHO85_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCQ2670171.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(68285..68776)
                     /gene="ilvN"
                     /locus_tag="OHO85_000749"
     CDS             complement(68285..68776)
                     /gene="ilvN"
                     /locus_tag="OHO85_000749"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCQ2670172.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(68779..70503)
                     /gene="ilvI"
                     /locus_tag="OHO85_000750"
     CDS             complement(68779..70503)
                     /gene="ilvI"
                     /locus_tag="OHO85_000750"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCQ2670173.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            70622..70666
                     /locus_tag="OHO85_000751"
     CDS             70622..70666
                     /locus_tag="OHO85_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670174.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(70821..71765)
                     /gene="leuO"
                     /locus_tag="OHO85_000752"
     CDS             complement(70821..71765)
                     /gene="leuO"
                     /locus_tag="OHO85_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCQ2670175.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT        17695 a        19412 c        17470 g        17429 t
ORIGIN      
        1 tatttttata ttattccctg ttttaattaa ctctgtcagg gatggtttat gttttttaac
       61 accaaacata caacagcttt atgcttcgta acctgtatgg cttttagttc atcgtctatt
      121 gcggacattg tcatttcggg tactcgcgta atatataaaa gcgatcaaaa aagtgtcaac
      181 gtacgtctgg aaaataaagg gaataacccg ttgcttgtcc agagttggtt agatactggc
      241 gatgacaacg ctgagcctgg cagtattaca gtccctttta ctgccacgcc gccagtatcg
      301 cgtattgatg ccaaacgtgg gcaaacaatc aaattaatgt acacagccag cacctcactg
      361 cctaaagaca gagagagcgt gttctggttt aacgtactgg aagttccacc aaaaccagat
      421 gcagaaaagg tcgcgaatca aagtctgctg caactggcat ttcgcacacg tataaaactt
      481 ttctatcgcc cggatggatt gaagggaaat ccctctgaag ccccgttagc cctgaagtgg
      541 ttctggtcag gttcagaagg taaggcgtca ttgcgagtga ccaatccaac cccttactac
      601 gtctctttta gcagtggtga tttagaagct agcggtaaac gctatccgat tgatgtgaaa
      661 atgattgcac catttagtga tgaggtcatg aaagtcactg gccttaatgg caaagcaagt
      721 tctgcaaaag tacattttta cgccattaat gattttggtg gcgccattga aggtaatgcc
      781 agtctgtaat caggcaagga tataattccg caggaagcat aacgtgacta taaaaccaac
      841 taaaaattat cataatcatc tgacccgtat cgctacgttt tgcgcgctgc tgtattgcaa
      901 tagtgctttc tgtgctgaac ttgttgaata tgaccatacc ttcctgatgg ggcagaatgc
      961 ctcaaatatt gatctcagcc ggtatagtga aggtaaccct gctataccgg gtatgtacga
     1021 cgtcagtgtt tatgtcaacg atcaacctat tattaaccaa agcattacat ttattgaaat
     1081 tgaaggaaaa aaaaacgcac aagcctgtat cacacttaag aatttattgc agtttcatat
     1141 aaattctccc gatataaata acgaaaaagc cgttctgctt gccagggatg aaacactcgg
     1201 caattgcctt aatttgacgg aaattatccc tcaggcttct gttcgttatg acgttaacga
     1261 gcaacgtctg gatatagacg ttcctcaggc atgggtaatg aaaaattacc aaaactatgt
     1321 tgatccatcg ttatgggaaa atggcattaa tgcggccatg ttgtcataca acctcaacgg
     1381 atatcatagt gaaactcctg gtcgaagaaa tgacagcatt tatgctgctt ttaacggtgg
     1441 gatgaattta ggtgcatggc gactgcgtgc ctcaggcaac tacaactgga tgaccgattc
     1501 tggcagtaat tatgatttta agaatcggta tatacagcgt gatatcgcct cgctgcgttc
     1561 tcaactcatt cttggtgagt cttatacaac gggcgaaacc tttgattctg tcagtatccg
     1621 cggcattcgt ttatacagcg acagccgcat gttgcctccg acattagcga gttttgcgcc
     1681 tatcattcat ggcgttgcta ataccaacgc caaagtcact attacgcaag gtggctataa
     1741 gatttatgaa acgactgtgc cgccaggcgc tttcgtcatt gatgatctga gtccgtcagg
     1801 ttacggcagc gatcttattg ttactgtcga agaatccgat ggctcaaagc ggacattctc
     1861 gcaacctttt tcatcggtcg ttcaaatgtt acgccctggc gttggacgtt gggatattag
     1921 cggcggccag gtcttaaaag atgatattca ggatgagcct aatttatttc aggccagcta
     1981 ctactacggt ctgaataact atctaacggg ttatactggt attcagatta ccgataataa
     2041 ctataccgct ggtttgttag gtcttggtct gaatacttca gtcggtgcat tttctttcga
     2101 tgtgactcac tccaatgttc gtataccgga tgataaaaca tatcagggac aaagttatcg
     2161 tgtttcctgg aacaagttat tcgaagaaac aagtacttca ctgaatatcg cggcctatcg
     2221 ctattcgaca cagaattacc ttggtcttaa tgatgcacta actctaattg atgaagtgaa
     2281 acatcccgaa caagatcttg aaccgaaatc catgcgtaat tactcgcgca tgaaaaatca
     2341 ggttacggtc agtattaacc aaccgctaaa atttgagaaa aaagattacg gttcatttta
     2401 tctttccgga agttggtccg attactgggc ttccggacaa aatcgtagca attactctat
     2461 tggctacagt aacagtgcat cctggggcag ctacagtgtc agtgcccagc gttcatggaa
     2521 tgaagacggc gacactgacg atagcgttta tctcagtttc accattccta ttgaaaaatt
     2581 acttggcact gaacaacgga cttcaggttt ccagagtatt gatactcaaa tgagcagtga
     2641 ctttaagggt aataaccaac tcaacgtcag tagcagtggc tatagtgata acgctcgcct
     2701 cagttatagc gtaaatactg gctatacgat gaataaagcc agcaaagatt tgagttatgt
     2761 tgggggttat gccagctatg aatcaccgtg gggaaccctg gcaggttcag tttctgcaaa
     2821 tagcgataac agtcgtcaag tgtctctcag caccgacggt ggttttgtat tgcatagcgg
     2881 cggcctgacc ttcagtaatg acagttttag cgactccgat acactggcgg tagttcaggc
     2941 tccaggtgct caaggagcgc gaataaatta tggcaacagt actatcgatc gatggggtta
     3001 tggtgtcacc agcgctcttt ctccttatca tgaaaaccgt atcgcgctgg atatcaacga
     3061 tcttgagaac gatgttgaat taaaaagtac cagtgcagta gctgtaccgc gtcagggttc
     3121 agtcgtcttt gctgattttg aaaccgtgca agggcaatca gccattatga acatcacacg
     3181 aagtgatggt aaaaatattc catttgctgc agatatttat gatgagcaag gcaatgtcat
     3241 tggtaatgtt ggacagggtg gacaagcatt tgttcgtggc attgagcagc agggaaatat
     3301 cagcattaaa tggctcgaag aaagtaaacc cgtaagttgt cttgcgcatt atcaacaaag
     3361 ttcagaagca gaaaaaatag cgcaatctat tattctgaat ggaattcggt gtcagattga
     3421 gtaactacaa gggacgtcaa atgataaaaa taacgccaca taaaatagcg atactgatgg
     3481 gaatattatt atcaccatca gtatttgcaa cggatattaa tgtagagttt acagccactg
     3541 tcaaagcgac aacctgtaac atcacactta ctggtaataa cgtcacgaat gatggcaata
     3601 ataactacac attgagaatc cctaagatgg gtctggataa gatcgcgaat aaaacgacag
     3661 aatctcaggc tgattttaaa ctggttgcta gtgggtgcag cagtggcatc agttggattg
     3721 ataccactct gaccggaaat gcatcatcaa gctcacctaa gcttattata ccgcagtctg
     3781 gtgattcatc ttcgacgaca agtaatatcg gtatgggttt caaaaaacgg actactgatg
     3841 atgccacttt ccttaaacct aacagtgcgg aaaagatacg ctggagcaca gacgagatgc
     3901 agcccgataa gggtcttgaa atgaccgttg cgctacgtga aacagatgca gggcaaggcg
     3961 taccggggaa ttttcgtgca ctggcaacgt ttaatttcat ctatcaataa gaaggataat
     4021 taagatgaca tttaaaaatt tacgttatgg attatccagc agtgtcgttt tggctgcctc
     4081 attgttcagc gtaccctctt atgcggcaac agacagcatt gggctgaccg tgattactac
     4141 cgtagaaata ggtacttgta ccgctacatt agtaaatgac tctgatcagg acatttctgt
     4201 tgttgacttt ggcgatgtat atatttctga aatcaatgcc aagaccaaag ttaaaacatt
     4261 caaactcaaa ttcaaagact gtgcgggtat ccccaataaa aaagcacaaa taaaattaac
     4321 caagcgagcc acatgcgaag gtactgctaa tgacggtgcg gggttcgcaa atggatccac
     4381 agccgcagat aaagcaagtg ccgtcgccgt tgaagtctgg agcaccgaaa ctccggcaac
     4441 aggtagcgca acacaattta gttgtgtgac accagcatct caagaggtca ctatctccaa
     4501 tgcagccaac gcggttgttt attatccgat gagtgctcga ctggtcgtcg aaaaaaataa
     4561 aaccgtaagc aatgtcactg cgggtaaatt ttctgcacct gccacattta cagtaaccta
     4621 taactaacag actgacagat ccaggaacta tatatgcatc ccactcaacg taagctgatg
     4681 aagagaataa ttctgtttct gtcactacag tttcccatta cctatccagc catcgctggg
     4741 caggatattg accttgttgc caatgtaaaa aacagcactt gtaaaagcgg aatcagtaac
     4801 cagggtaata ttgatcttgg cgtcgttggg gtgggatatt tttcagataa tgttactcct
     4861 gaaagctatc aaccaggtgg aaaagagttc actgtcacag tatcagactg tgcattacag
     4921 ggaactggcg atgtgcttaa tcagttacat attgatttta gagcccttag cggtgtcatg
     4981 gctgcaggtt caaggcaaat atttgccaat gaagtcgcaa ccggggctaa aaatgtcggt
     5041 gtcgttctct tttccattca ggacccgacc aatatattca atgttattag ttcagcagga
     5101 aattctcgtt ccgtgtatcc agtaatgact tctgccctgc ataattcttc atggaaattt
     5161 tatgccagaa tgcaaaaaat cgatcccgcg ctggatgtca tatctggcca ggtaatgagt
     5221 catatattag ttgatgtgta ctacgaataa tagatcaaaa caaataactg cgactgttac
     5281 agcagtagat ggtggagtga taatgaagaa attcttcaga cattttcttt ttttaattct
     5341 gtgcttgagt tgttataccg cgagtgctgg cactgatgat aacgtaggat atattgttgg
     5401 gaatagttac ggcgttggtc cgtcagatca aaaatggaga gaaacggggc cgaatggcga
     5461 tgctactgtc atattcagat atgcaacaag tacaaataat ttagttttct acaaaccgac
     5521 gcagcttgga cctacaggtg taaaattaca gtggagtcag ttagataccg cttctggtgg
     5581 tggttttctt tattgcaaca gaagtgatag cacaagtggt agcgcaatgc gtattgaaaa
     5641 tgcaatggtt gactcaggta aaatgtatgg ctcccataaa ttatttaata catcagttcc
     5701 tggtttgtat tacacattat taatttcaaa catgtggtca gcttacggta ccgtaactaa
     5761 cgttagttca cctgggatat acattggtga ctctgcagaa caatattttt cgtggtataa
     5821 tccaagcgaa gacgtgttat attggagttg caataatgcg aatagcaccc gtaaatactg
     5881 ggctgtaggt ggtatttatc agacccttac aattgaattc tatacagata caaactttga
     5941 tccaacagta actcaacaga tcaaattatc cagctcatca aattatctgt attcatttaa
     6001 agcctatggc gcaggccaag gtataaatga gcatagttat tttatcaaaa tcgatttcga
     6061 tctgctcaat gtcaagttga ctaatcccac ttgttttacc gctatgctta gtggaacttc
     6121 tgtgactggt tctacggtaa aaatgggtga atatagtgca gaacaaatca gaaacggtgc
     6181 cacaccggtt ccttttgata tttcacatca aaactgcgtt cgtgtgacta atattgagac
     6241 aaaattagtt tcaacaaagg ttggtactga aaacgggcaa ctccttggta atactctcac
     6301 aggtaatgac gcagcaaaag gagtcggtgt actcatagaa ggtttagcaa ctagtaaaaa
     6361 tcctctaatg acattgaaac ctaatgattc aaattctgtt tataaagatt acgacccgag
     6421 aggcaaagac gatacaacag gaggagttta cccggatcaa gatacaggta taacataccc
     6481 tctccatttc caggccacgc tacaacagga tggaactata ccaatagaag ctggtgagtt
     6541 taaagccacc agtactttcc aggtaaccta cccttaataa gtccaccgca ccgccatcat
     6601 ctggcggtgc agtaattgat aaatcattgc ccccaccaat gtgactgata aaatgccagg
     6661 caacacgaca tcatttatca ggatacgtta tgaaaccaac caccattgct tcacttcaga
     6721 aatgtaagca ggataaaaag cgtttcgcga ccatcaccgc ttacgactac agcttcgcga
     6781 aactatttgc tgatgaagga cttaacgtca tgctggtagg cgactcgctg ggcatgacgg
     6841 ttcagggtca cgactccacc ctgccggtga ccgttgctga tatcgcctac cacactgccg
     6901 ccgtacgtcg cggcgcgcca aactgcctgc tgctggctga tctgccgttt atggcgtatg
     6961 ccacgccgga acaagccttt gaaaacgccg caacggttat gcgtgccggt gccaatatgg
     7021 tcaaaattga gggcggtgag tggctggtcg aaaccgtaaa aatgctgacc gaacgtgccg
     7081 ttcctgtatg tggtcactta ggtttaaccc cacagtcagt gaatattttc ggtggctaca
     7141 aagttcaggg gcgcggcgat gaagcgggcg atcaactgct cagcgatgca ttagccttag
     7201 aagccgctgg ggcacagctg ctggtgctgg aatgcgtgcc ggttgaactg gcaaaacgta
     7261 ttaccgatgc actggcgatc ccggttattg gcattggcgc aggcaacgtc actgacgggc
     7321 agatcctcgt gatgcacgac gccttcggca ttaccggcgg tcacattcct aaattcgcta
     7381 aaaatttcct cgccgaaacg ggcgacatcc gcgcggctgt gcggcagtat atggctgaag
     7441 tggagtccgg cgtttatccg ggcgaagaac acagtttcca ttaaggagtc acgttgtgtt
     7501 aattatcgaa accctgccgc tgctgcgtca gcaaattcgc cgcctgcgta tggaaggcaa
     7561 gcgcgtggcg ctggtgccta ctatgggtaa tctgcacgat ggccatatga agctggtcga
     7621 cgaagccaaa gcccgcgccg atgtggtcgt cgtcagtatt ttcgttaacc caatgcagtt
     7681 tgaccgcccg gaagatctgg ctcgttatcc acggaccttg caagaggact gcgaaaagct
     7741 aaacaaacgt aaagtggatt tagttttcgc cccttcggta aaagagatct acccgaacgg
     7801 tactgaaacc cacacttacg ttgacgttcc tggcctttcg accatgctgg aaggtgccag
     7861 ccgtccggga cattttcgcg gcgtttcgac tattgtcagc aagctgttca acctggtcca
     7921 gccggacatc gcctgcttcg gtgaaaagga ctttcagcaa ctggcgctaa tccgcaaaat
     7981 ggttgccgat atgggcttcg atattgaaat tgtcggcgtc ccgattatgc gcgccaaaga
     8041 tggccttgcg ctcagttccc gtaatggtta tctgacggcg gaacaacgca aaattgcgcc
     8101 cggtctgtac aaagttttaa gttcaattgc cgacaaattg caggctgggg aacgggatct
     8161 cgatgaaatt attaccattg cggggcaaga actgaatgaa aaaggcttcc gcgccgatga
     8221 tattcagatt cgcgatgccg acacattgct ggaagtttct gaaaccagca aacgggcagt
     8281 aattctggta gccgcctggc ttggcgatgc tcgcctgatc gacaacaaaa tggtcgagct
     8341 ggcgtaatac ttaactggcg ctacggctga tggcgccagt tattaattta ccccacgtca
     8401 tctgtcataa cacaggtaac ttaaataagg ttaattacct tatcaatctc agcaagagat
     8461 aaatttgtca tctctgcaac gtcttcccga gacattcctt tacttagaag acgcagggcg
     8521 aattcttgcc tttcctcctg tcttccctgc tgaatcccct tctcaatccc tttctcttca
     8581 aaccactgcg ccagcgtcat catagactcc cctcccgttt ccctgtctct cagcacacca
     8641 taaaacaaat ccgcttgttc agtatgaccg cgttgcagca tatagttttg catggcaact
     8701 aactgacttc cgctagtgta cccttcgtca atcagcgtga ccagttgctc cagcaataac
     8761 attaagtcgc gctggcgaat atgtttttgc agtagttcga gaatcgcaat ccgccgatgt
     8821 tgcatgattt cgtcatccgg tgtgatggtg atatccacca gcgggaaagg actgttatag
     8881 acgcgtcgtg ccagctccgg cgagtaaaat atatcaaacc agcacattga gagcggataa
     8941 ggtgtggcct cgccctgata aaacagtatc ggcaccacca gcggcagctt atcgtgatcc
     9001 gcctccagat gccggtgcat ggcagcaata gaataacgca tcatgcgaaa ggccattttc
     9061 ttatccggct tgctttggtg ttcaatcaca acatgcagat aaccgggatt gccctgcatt
     9121 tgcacggaat agagcacgtc cgtgctgtgt cctttcaggc tctcttcaat gaaactcccc
     9181 gactctaaat gaagcgtgtt gaggtcacaa agttcgcgta attccactgg caaatgtatc
     9241 tccagaaagt cgcgagccgt ctccgcatgc attaaaaatt gtttaaatac cgcgtcatgc
     9301 ggtgtggtac tcggtgcatc catcttccct gtcttccgtg ttgtcttgcg tgaaggcagt
     9361 atccagtcaa ggcgcgccgt cgtcagccgc ctctttagtg tttaccgagc agcgttcaga
     9421 gaaatttcaa ttcgttacag gcgatacatg gcacgcttcg gcgcggtacg caaactgctg
     9481 acgcaaatgc catcagatga gtagacactc atcaaaaatc gggcaatact gcgtgagaat
     9541 ttcctcaaag caggcaccgc ctgcttcgtt aacgacaggg tagaaaggta gaagttatga
     9601 ttcgcacgat gctgcagggc aaactccacc gcgtgaaagt gactcatgcg gacctgcact
     9661 atgaaggttc ttgcgccatt gaccaggatt ttcttgacgc agccggtatt ctcgaaaacg
     9721 aagccatcga tatctggaat gtcaccaacg gcaagcgttt ctccacttat gccatcgcgg
     9781 cagaacgcgg ttcgagaatt atttctgtta acggtgcggc ggcccactgc gccagcgtcg
     9841 gcgatattgt catcatcgcc agcttcgtta ccatgccaga tgaagaagct cgcacctggc
     9901 gacccaatgt cgcctatttt gaaggcgaca atgaaatgaa acgtaccgcg aaagcgattc
     9961 cggtacaggt tgcttgattg ttatccctgc ggctggttac tcatcagccg cgacatcgtc
    10021 tccagcgaat ccgttcttaa gatataaagt cgttttagta acaacggatt atcccccggt
    10081 tttactttgc ctttcatggt tgtcaccgcc aggtgaaatc cggcatcgtt tgctgccttc
    10141 acggctttgt cattaaatcc gccaaacgga tacgaaagat accagacatg cggattaaat
    10201 tgcgccagag cgcggcgtga acgtgcaaaa tcaaacagaa tattgtgctc actacggctc
    10261 agtaatatgg gtcggcgata gccatccacc cgatgcaaaa aatgggtatg tgactgaaaa
    10321 tcaaaaacat cgcgaatttc gttaagctca gaaacgctca taaattgcag cgattttggg
    10381 ttccacttct gcgggtgacg tttgatgcgc gaggtaacaa caaacgccgt cgccttcatg
    10441 ccatattgtt tcaatacagg atacgcatag cggctcaccg acttgaggcc atcatcaaag
    10501 gtaatcacca ccgctcgcgc agggagattg atcttattct tcacgtaacc ttccagctgc
    10561 gccatgctca gtgtcgcgta tcccctgtcg cgcagccagg ccatctggtt attgaaagcg
    10621 cgtactgagg tggtcgtcga agtatggcga aaacgggtgt tttcttcatc gcgcagaata
    10681 tggtgatagg ttagcaccgg caggccatta tcgggttggg catccagcgc gctgatatag
    10741 gcgagtcgat cgccaatacg gatctgatac caggtttgat ttaacctgtc tttcagttta
    10801 tgcaaaatcg ggtagcgcaa attgtccgcc agtaccccaa atggcgcact gcccgcactc
    10861 ggcgcgttat agaccggcgt atctttccag gtaattaagt tctgattact cagcggcttg
    10921 ttgaggtcac ccaaaccgtc ttcaactttt tgtcgcccct gaactggctc gagatgccct
    10981 ttatcgataa accctttacc aaagccaaaa ttaaatgcgt aataacttgc ggcagtgggc
    11041 tccacggcaa tgatttgccc ggcccgaata ttccccacgg tcaccatctt gtcaccaatt
    11101 tgcgcccaga ccgcagcatt ttcgatggtt tgcatataac gggcaggtaa cgcggcacta
    11161 acgcttgcag taaacagcat cagcaggaga ataacagctt gtttgtacat gaaaaaataa
    11221 ccgagggtaa aacaacgctt ttattgtagc aaaagcgcca tcagaatcgc gggttaatac
    11281 tcatacaaat tatgtttgag gacgaaaggc ggattttttt gttgttggtg ccaaagacta
    11341 ctcacctccg gcgcacccag ttcgacaata cgctcgcgaa actctgaact ggtcatggtt
    11401 tcacggcaag ccatcatctg ttcaattaac ggtaacgtga caccagaaat cacttcgcaa
    11461 cgggagtgtt tgtggcttaa taatgaagcc acgcggtacg gtggcgctcc ggctatatcg
    11521 gttaagaaga tgacaccctc accagagtcc gcttcatgca gagcatcgca catcatgcgg
    11581 ctgagcatat tagagcttaa cccacgccag aaattcacgg cccggcactg aagaagcgcc
    11641 ccatgttttt tctccagcgc atccagtatc tcttgcgccc ggtcatcgtg acaggtaatt
    11701 acccaaccta acatcgcctt cctccttagc cgaaaatcag tctaccctga ccattgttga
    11761 taaagggtga tagccgtcaa aactcctctc ccccaaatcc agaggagagg aaatagcctt
    11821 agctacgcaa accacgtcca cgttggatca gcgaccaaca gatcaaataa aacgccacaa
    11881 taaagaccac cagtacgcca aaggtagtga ccagcggaac atcattgata ccgaggaagc
    11941 cgtagcggaa accactgatc atataaacga ttgggttcag gtgcgacagc ccttgccaga
    12001 acggcggcaa caaagtcagt gagtaaaaga ccccacccaa atacgtgagt ggcgttaaca
    12061 caaaggttgg caccaggctg atgtcatcga acgttttggc aaacacaccg ttcagcaaac
    12121 ccgcaaggga gaacaacacc gccgtgagca ccagggttaa ggcaacgaat acccacgaat
    12181 gcacctgaaa cggcacaaaa aacagtgaaa ttgccgtcac cagaatgcca acaaacagac
    12241 cacgcgccac gccaccgccg acatatccgg caataatgac gtgagtcgga accggcgcta
    12301 ccagcagctc ttcaatatta cgctggaact tggcaccaaa aaatgatgac gcaacgttgg
    12361 cgtaggcatt ggtgatcacc gacatcatga tcagccccgg tacgatgaac tgcatatagc
    12421 tgaagccatg catatcgcca atacgcgaac caatcaggtt accgaagata ataaagtaaa
    12481 gggtcatggt gatgactggc ggcaccagcg tctgcaccca gatacgcata aagcgatgga
    12541 tctctttcgc ccagatgctt tttagcgcca cccagtaaag atgcatcatg cgcgatctcc
    12601 ttgtttttca ttaaccagtg aaacaaacag ctcttccaga cggttagctt tgttacgcat
    12661 acttaatacc tgaatgccct gctcacttaa ctgcgtaaat acgctgttga tcccctgctc
    12721 acgcagcact tcaacttcca gcgtcgcggt atcgaccagt cgatactgat agccatcgag
    12781 cttcggtaac ggacttttcg gtgcgagatc gagaataaag gtttccgatt tcagcttcgc
    12841 cagcagcgcc ttcatcgagg tattttccac cagctcaccg tgttgaataa tgccgatatt
    12901 gcggcacagc atttctgctt cttccaggta gtgtgtggtg agaatgatgg tggtgccttt
    12961 gtcgtttaaa tccttcaaaa agccccacat tgagcggcga agttcaatat ccacgcctgc
    13021 ggtcggttcg tcgagaatca gtagtttagg ttcatgcatt aacgcacggg caatcattaa
    13081 acggcgcttc atcccgccag ataacatacg cgcacgttcg ttgcgttttc cccatagatc
    13141 gagttgttta agatattttt cgctgcggat gtacgcctct ttgcgctcca caccgtagta
    13201 ccctgcctga ttcaccacaa tttgctgcac ggtttcaaac gggttgaagt taaattcctg
    13261 cggcaccagt cccaactgac gtttagcgtt cacgacatct ttctcgagat cgtaaccaaa
    13321 tacgctgacc cgcccggagg ttttatttac cagagagctg ataataccga tagtggtcga
    13381 tttcccggcc ccgttcggcc cgagaagcgc ataaaaatca cccgcttcga cctgcaaatc
    13441 tattccacga agcgcctgaa cgccgcctgg ataggttttt ttaagctgtt gaagttccag
    13501 tgcaatggtc ataaattttt acttacctta cgttcttaca ctttatctgt ggtttaaatc
    13561 gtcccggagt tgccctatat tagccaaacg taattatttg gttacaggtc gttaacctcc
    13621 atgaaagaca tagatacact catcagcaac aatgcactat ggtcaaaaat gctggtggaa
    13681 gaggatcccg ggttttttga gaaactggca caagcgcaaa aaccgcgctt tctatggatt
    13741 ggatgttccg acagtcgcgt tcctgcagaa cgtttaaccg gtcttgagcc gggcgaactc
    13801 tttgttcacc gtaatgttgc taacctggtc attcacaccg acctgaactg cctttccgtg
    13861 gttcagtatg cagtggatgt actagaagtt gaacacatta ttatctgtgg ccactacggt
    13921 tgcggcggcg tacaagccgc agttgaaaac ccggaactgg ggcttatcaa caactggctg
    13981 ctgcatatcc gcgatatctg gttcaaacat agctcattgc tcggcgaaat gccgcaagag
    14041 cgccgtctgg ataccttgtg tgaactgaac gtcatggaac aggtgtataa cctgggccac
    14101 tccaccatta tgcaatcagc gtggaaacgc gggcagaaag ttaccattca cggctgggcc
    14161 tatggcattc acgacggctt gctgcgtgat ctggatgtca cagcgactaa ccgcgaaacc
    14221 cttgagcaac gttaccgtca cgggatttcc aacctcaagc tgaaacacgc caaccacaaa
    14281 taaaaatgcc atgccggatg caacacatcc ggcaacttca cacttactcg tccagcagaa
    14341 tcactttgcc gatatacggc agatgacggt aacgctgtgc gtaatcaatg ccgtaaccca
    14401 ccacaaactc atccgggatc gagaaaccga taaattctac cgggacgttc acttcacgac
    14461 gggacggttt atccagcagc gtacaaatcg ccagcgactt cggttcgcgc aggcttaaga
    14521 tctcacgcac tttcgacagt gtattccccg agtcgatgat atcttcaaca atcagcacgt
    14581 ccttgccacg gatatcttca tccagatctt tgaggatttt cacatcacgg gtggtggaca
    14641 tgccgctacc gtagctggag gcggtcataa agtcgacttc atgagatacc tgaacttcac
    14701 ggcacaggtc cgccataaac ataaatgagc cacgcagcag accaaccagc accatatcgc
    14761 tgccgctgtc tttgtaacgc tcagtaatct gacgacccag ttcggcgata cgcgctttaa
    14821 tctccgcttc ggggatcatt acttctacag tatgtttcat atctctaacc atatgattta
    14881 aaaacaaatc acttcgcacg ggcaggtttt ggcaccgcaa caaagcgcaa gacacgcagt
    14941 ataccagtaa aactattcat tcgcggaatc tgttaataaa gctcgaattg tgatgacgat
    15001 cacacatgtt aaaacctata attaattgct actaaaatat taatgaattg aaatggtgtc
    15061 tctttatggc aattaacaat acaggctcgc gacgattact cgtcacgcta acagcccttt
    15121 ttgcagcgct ttgcgggctg tatctactca ttggcggagg ctggctggtc gcgattggcg
    15181 gctcctggta ctaccctatc gctggtcttg tgatgctcgg cgtcgcctgg atgctgtggc
    15241 tcagtaaacg tgccgcgctt tggctgtacg cagccctgct gctcggcacc atgatttggg
    15301 gcgtctggga agttggtttc gacttctggg cgctgactcc gcgcagcgac attctggtct
    15361 tcttcggcat ctggctgatc ctgccgtttg tctggcgtcg cctggtcatt cctgccagcg
    15421 gcgcagttgc cgcactggtg gtggcactgc tgattagcgg tggtatcctg acctgggccg
    15481 gatttaacga tccgcaggaa atcaacggca ccttaagcgc cgatgccaca cctgccgaag
    15541 caatctcccc cgttgccgat caggactggc ctgcctatgg tcgtaatcag gaaggtcaac
    15601 gcttttcacc tctgaaacaa attaacgccg ataacgttca caagctgaaa gaagcctggg
    15661 tgttccgtac tggcgatgtg aagcagccga acgatccggg tgaaatcacc aatgaagtga
    15721 cgccgattaa agtgggcgac accctttacc tgtgtaccgc tcaccagcga ctgtttgcgc
    15781 tcgatgccgc cagcggcaaa gagaaatggc attacgatcc tgagctgaaa accaacgagt
    15841 ctttccagca cgtaacctgc cgtggcgtct cttatcatga agccaaagca gaaacggctt
    15901 cgccggaagt gatggcggat tgcccgcgtc gtatcattct tccagtgaat gatggtcgcc
    15961 tgattgcgat taacgctgaa aacggcaaac tgtgcgaaac cttcgccaat aaaggcgtgc
    16021 ttaatctgca aagcaatatg ccagacacca aaccgggtct gtatgaaccg acttcgccac
    16081 cgattatcac cgataaaacc atcgtgatgg caggttcagt taccgataac ttctcaaccc
    16141 gcgaaacgtc tggcgtgatc cgtggttttg atgtcaacac cggggagctg ctgtgggctt
    16201 ttgatccggg cgcgaaaaat ccgaacgcaa tcccgtctga cgaacacacc tttaccttta
    16261 actcgccaaa ctcgtgggca ccagcggcct atgacgcgaa gctggatctg gtgtatctgc
    16321 cgatgggcgt gaccacgccg gatatctggg gcggtaaccg cacaccggaa caggaacgtt
    16381 atgccagctc gattctggcg ctgaatgcca ctaccgggaa actggcgtgg agctaccaga
    16441 ccgttcacca cgacctgtgg gatatggatc ttccggcaca gccgacgctg gcggacatca
    16501 ccgttaatgg tcagaaagtg ccagttattt acgctccggc gaaaaccggc aacatttttg
    16561 tgctcgatcg tcgtaatggc gaactggtgg ttccggcacc ggaaaaaccg gttccccaag
    16621 gtgctgccaa aggcgattac gttaccccta ctcaaccatt ctcggaactg agcttccgtc
    16681 cgacgaaaga tttgagcggt gcggatatgt ggggagccac catgtttgac caactggtgt
    16741 gccgcgtgat gttccaccag atgcgctatg aaggcatttt caccccgcca tctgaacagg
    16801 gtacgctggt cttcccgggt aacctgggga tgttcgaatg gggcgggatt tccgttgatc
    16861 caaatcgtga agtggcgatt gccaacccaa tggcactgcc gtttgtttcg aaactgatcc
    16921 cgcgtggtcc tggtaacccg atggagcagc cgaaagatgc caaaggcacg ggtacggaat
    16981 ccggcattca gccacagtac ggtgtaccgt atggtgtcac gctcaacccg ttcctctcac
    17041 cgtttggtct gccatgtaaa cagccagcat ggggttatat ctcggcgctg gatctgaaaa
    17101 ccaatgaagt ggtgtggaag aaacgtattg gtacgccgca ggacagtatg ccgttcccga
    17161 tgcctgtacc ggtgccgttc aatatgggta tgccgatgct gggcgggcca atctccacgg
    17221 cgggtaacgt gctgtttatc gccgctacgg cagataacta cctgcgcgct tacaacatga
    17281 gcaacggtga aaaactgtgg caaggtcgtt taccagcggg tggtcaggcg acgccaatga
    17341 cctatgaagt gaatggtaag cagtatgtgg tgatctccgc aggcggtcac ggttcatttg
    17401 gtacgaagat gggcgactat attgtggctt atgccctgcc ggatgatgtg aagtaagact
    17461 tgctcagatt gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtta acgccttatc
    17521 cagcctacaa aattgtgcaa aatcaatgga ttgcacaggt agcgtaggcc tgataagcgt
    17581 agcgcatcag gcagatttgc gtttgtcatc agtttaatgc ccggagagat ccgggcatat
    17641 ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc ttcatgctcc
    17701 agcagatggc agtgcgccat ataagcatgt tctttcggtg catcgtgatt aaacttcacc
    17761 agcacttcgc tgacattacc ttctacctta acggtatctt tccagcccgc gcgatgagcc
    17821 gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat atggaacgga
    17881 tgcagcatca tgtcacccac gccagagata acccaacgtt cgtactgccc tttcgccgcc
    17941 gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc atggtggaaa
    18001 tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg gcccatcatc
    18061 tggctgtgat ccatcccggc catcgcctga tcgccatatt tctccatcag catctgcata
    18121 cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt cagcccttcc
    18181 agcgaaggta acgcaggcag gctacttaat gtgtctggca aagcaccgga ggcactaata
    18241 gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggcgcaat cgccatcccc
    18301 atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac ctccaccagc
    18361 acttcaaaac gctcgcccat cagcaccggc agctcgttca ccttcaccgg ttcaggtagc
    18421 agaccaccgt cgctggcaat cacataaagc gggcgattgt cgctggtggc gaaattgagc
    18481 gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg ggcagcgtgt
    18541 tgcgggtaga ttgcaccgtt ggtcagcaac gtatcgccaa accagcccac ggcggcggtc
    18601 atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc ctgaacgatc
    18661 accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat ctcgtcatct
    18721 tcaattacca ccaacccagc caaccccatc gccacctgtc gcccggtctt gccatgttga
    18781 tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac cgagcgcttg
    18841 ccacctggcg gaataattcc ctgcgggccg ccgtccactt cacccggtac ttccagcccg
    18901 tgccagtgca acgtcgtctc ttccgtcagt tggttataga tatcaaccgt taccgctttg
    18961 ccgtgctgta atttcaccgc cggccccagc agattgccgt tatagcccca ggtagttgca
    19021 gttttcccgc caaaggtgga ctggcctgcg ccaatagtta actgaatgcg attacgggca
    19081 tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa gactgcgcgg
    19141 ctccacagcg gcaaggctga agccacaccc agcgcgacgg aatattttaa gaaatcacgg
    19201 cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg ttgagcatag
    19261 tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat aatggtcgtc
    19321 gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa gcaatgaaga
    19381 cgtttttcag aacagtgtta ttcggcagcc tgatggccgt ctgcgcaaac agttacgcgc
    19441 tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc tttctgaaga
    19501 acgattgtgg ttaccagaac ttacctaacg ggcaaattcg tcgcgcactg gtctttttcg
    19561 cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa gccctcggtg
    19621 aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaag tgcaaagccc
    19681 tggctcgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata cctttttgta
    19741 gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc tttttttacg
    19801 ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat gaaacgctac
    19861 acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa aagaccgatc
    19921 accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg ctggatggcg
    19981 tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgact catgttccgc
    20041 tactgaccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac ggtgccatgc
    20101 tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa atcgatgcgg
    20161 gtgtcgtatc gttctgccgt cagtatctac ccaaccataa cgccggtagc tacgacgatc
    20221 cgcgctttaa gctggtgatc gatgatggcg tcaatttcgt taatcaaacc agccagacct
    20281 ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagt ctgttcacct
    20341 cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc gtcgcacaaa
    20401 acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa ctcagccatt
    20461 acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc ggtatcatga
    20521 cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa attattcagg
    20581 cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc catacggcag
    20641 cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc taaggagaag
    20701 ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa agtctgagtt
    20761 tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt tatattgctt
    20821 atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa acctgttcca
    20881 ttatcggggc caatattctt aacatcgccc gtcaggatta cgaaccacag ggtgccagcg
    20941 tcactattct ggtgagtgaa gaaccggtcg acccgaaact tatcgacaaa acagaacacc
    21001 ccggcccact gccagaaacg gtggttgccc atctcgataa aagtcatatt tgcgtacata
    21061 cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat attgaagtct
    21121 ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac cagcttgagt
    21181 ccgatatcgt aaccattgat tatcgcgtgc gcggttttac ccgcgacatt aacggtatga
    21241 agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac gatatgaagg
    21301 cgctgtatga catggtggat gtgaacgtct atcaggaaaa tatcttccat accaagatgt
    21361 tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa gatttaaccg
    21421 acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag atttattacg
    21481 ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg ccagatttat
    21541 ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat cctcaacgcc
    21601 gcatagcgac aggctttctt cgtcgtagta gttcatccct tcttccattt catcgccagc
    21661 gaattccagt tgattggcgc gaaccatcac ctcttcaccg tccatccaca gggtgtattc
    21721 atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg cttgttccac
    21781 ttcatcaagc aaagccaggt tttctttcac ctcttcatta aaccagtgcc cgaccacttc
    21841 atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa attcgtaatc
    21901 catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg cattgcgcca ttgatgtaat
    21961 tatcgcaacc ttaccggaga aaaacagcgg aacggcgatg gtttagaaat aaaaaatgcc
    22021 cgggaatctc attccccgag cattgtgtcg tttatgcgca gcgcgtgcgc tgacttttta
    22081 aaccgcagtc tggaaaatca ccccatcggc tttttcggta tactgagaaa gctggttgaa
    22141 gttcagataa cgataagtat caacggcagt tttatccact tgcgccacgt aggtctggta
    22201 ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt ccgcagaagc
    22261 caggaagaca ttcgcgccag tacccagacg gttcgggaag ttacgggtag aggtggaaac
    22321 caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac agccagggat
    22381 ctcgatacgc gcaccactct taccgaagac gctgtagtag ccttcttcag tcaactgcgc
    22441 ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcagttggc ctttgtgcgc
    22501 atccagcagt ttacccgcag cacggaagtg accgatgttg gtcatgcagg aaccgataaa
    22561 tacttcgtcg atcttctcac cctgtaccgc agacagcgga cgcgcgtcat ccgggtcgtt
    22621 cggtgcacag aggattggct ctttaatatc cgccagatcg atgtcgatca ctgccgcgta
    22681 ttcagcatct gcatcggctt ccagcagctc aggattcgcc agccattttt ccatgccctg
    22741 aatacgacgt tccagggtgc gacgatcgcc gtaaccttcc gcgatcatcc acttcagcag
    22801 gacgatgtta gagttcaggt attcgatgat cggttctttg ttcagcttga tggtacaacc
    22861 agcggcagaa cgctcagcgg acgcatcggt aagctcaaag gcctgctcaa ctttcagatc
    22921 cggcagacct tcaatttcca gaatgcggcc agagaagatg tttttcttgc ctttcttctc
    22981 aacggtcagc agaccttgtt tgatggcata cagcggaatg gcgtgtacca gatcgcgcag
    23041 ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg gcatatccag
    23101 cggcattacg cctgttgcgg ctgcaaatgc caccagacca gaacccgccg ggaaagagat
    23161 accgatcggg aaacgggtat gggagtcacc tccggtaccg acggtatccg gcagcagcat
    23221 acggttcagc caggagtgga ttacgccgtc acccggacgc agcgacacac cgccacggtt
    23281 cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg gatacgccgc
    23341 ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg ccaggtcttt
    23401 cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag tcattttcgg
    23461 ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta ccattttctg
    23521 cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga acacatcact
    23581 gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga taatcagcgg
    23641 aatacggcca ccagcacgca cttcatcaat cagcacgtcg gttttcagtt cgaaggtcgc
    23701 cagcagttcg ccggtttcgt ggttacgcac ttcacctttg tacgggtaaa cgtcaatcac
    23761 gtcgcccatg ttcaggttag agacgtcgac ttcgattggc agtgcacccg cgtcttccat
    23821 cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc cgcgtttgtt
    23881 cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttagtgg cggatttacg
    23941 cgaagaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt tctgttgcag
    24001 agcttcgatt tgcttgatcg gaccaacaac accaggctgg tctggctcaa taccttcacg
    24061 ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc acgcatccgg
    24121 tgccggagaa aggtcatcgg tgttagtttc gccagtgact ttgaagacag taacggtcag
    24181 tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg actgcataac
    24241 ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga agttatcgaa
    24301 catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg catcatccag
    24361 cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca gttcgatggc
    24421 tttttccgga gtcagcagag gggatttggc ttcgcctttc gcgatagcag ccaggaagcc
    24481 tgctttgaca taggcggctt catcgacgcc tgggggaaca cggttggtta acagatctaa
    24541 caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagtg cggccatttg
    24601 gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta cgtgcttacg
    24661 gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc aggcgattct
    24721 cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc aacgggcaga
    24781 atagcaggat tttgacgatg tgttaatctg tttacaaaaa agcaacataa aaaagtggct
    24841 gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt aattcgcatg
    24901 gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact accgcctctt
    24961 tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga tttccttgag
    25021 atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct aatttgcagt
    25081 gccgggcttt gtgccgcctc taccgggcta tatataaaaa gccgcacagt ggaagcgcct
    25141 gtggaaccgc aatcgtcaca acagactgcg cctgacatca ccgcagttac gcttcctgca
    25201 acggtttccg caccgcccgt aacgcccgcc gtcgtcaaat ccgcattcag cactgcacaa
    25261 atcgatcaat gggttgcgcc tgtcgcgctg taccccgatt ctctgctttc acaagtgtta
    25321 atggcatcaa cctatccggc aaacgttgct caagcagtgc aatggtcgca cgataatcca
    25381 cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga cgccagcgtt
    25441 aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc gcaatgggtg
    25501 caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc ggtacagcga
    25561 ttgcggcaac tggcgcaaca aaccggttcg ctgaagtcat caaccgaaca aaaagttatt
    25621 accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt cataccatcc
    25681 aataccgttt taactgccag ccccgtcatt acagagcctg caacaaccgt catttccatt
    25741 gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt ggtttacggg
    25801 aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg agaaccgttt
    25861 attgacagct ttgtgcgcgg attcggctat agcatgggtg ttgctaccac gtacgcacta
    25921 ttcagcagca tcgactggga tgacgacgat catgaccatc atcatcgtga cggtaatggc
    25981 tggcaacaca acggcgacaa catcaatatc gacgtcaaca atttcaaccg tatcaccggt
    26041 gagcatctta ctgataagaa tatggcatgg cggcacaatc caaactaccg taatggtgtg
    26101 ccctatcatg atcaggatat ggcaaagcgg tttcatcaaa ccgatgtcaa cggcggaatg
    26161 agcgccacgc agctacctgc tccaacacgc gacagccagc gtcaggcggc agcaagtcag
    26221 tttcagcaac gaacacacgc cgcccccgtc attacacgag atacccaacg tcaggcagcg
    26281 gcacagcggt ttaatgaagc tgaaaactat gggagctatg acgacttccg cgacttcagc
    26341 cgtcgccaac cactgaccca gcaacaaaag gacgccgctc gtcagcgtta tcagtcggcc
    26401 tcgcctgagc agcgccaggc agtccgcgag aaaatgcaga ctaacccaca gatccagcag
    26461 cgaagagacg cagcgcgtga gcgtattcag tccgcctcgc ctgagcagcg ccaggcagtc
    26521 cgcgagaaaa tgcagactaa cccacagatc cagcagcgaa gagacgcagc gcgtgagcgt
    26581 attcagtcag cctcgcctga gcagcgccag gtgtttaagg aaaaagtaca gcagcgccca
    26641 ctgaaccaac agcaacgtga taacgcccgc cagcgtgttc aatcagcatc acctgaacaa
    26701 cgtcaggttt ttcgggaaaa agttcaggag agccgcccac aacgtctaaa cgacagtaac
    26761 catactgtca ggctgaataa cgagcaacgg tcagcagtac gcgaacgtct ctctgagcgc
    26821 ggagcaaggc gacaggaaag gtaaattgca gacgtaaaaa aagcggcgtg gttagccgct
    26881 tttttaattg ccggatgttc cggcaaacga acaattactt cttcttcgct ttcgggttcg
    26941 gcaggtcggt aatgctacct tcgaacactt ctgccgccag gcccacagac tcgtgcagag
    27001 tcgggtgcgc gtggatggtc agtgcgatgt cttcagcatc acaacccatt tcgattgcca
    27061 ggccgatttc acccagcagc tcgccgccgt tggtaccgac aatcgcacca ccgatcacac
    27121 ggtgagattc tttgtcgaaa atcagcttgg tcataccgtc tgcgcagtcg gaagcgatag
    27181 cacgaccaga agcagcccac gggaaggtgg cggtttcata gctgatgcct ttctctttcg
    27241 cttctttctc agtcagacct acccatgcaa cttctggttc ggtataggcg atggacggga
    27301 taactttcgg atcgaagtag tgtttcttac cggcgataac ttcagcggca acgtgacctt
    27361 cgtgaacacc tttgtgtgcc agcatcggct gaccgacgat atcgccgata gcaaagatgt
    27421 gcggtacgtt ggtacgcagc tgtttgtcaa cgcggatgaa accacggtcg tccacttcca
    27481 cgccagcttt gcctgcgtcg aggtttttac cgttcggcac acgaccaatc gctaccagca
    27541 cggcgtcgta acgctgcggt tcagcgggtg cttttttgcc ttccatcgtc acataaatac
    27601 cgtcttcttt cgcttcaacg gcggtaactt tggtttccag catcaggttg aatttcttgc
    27661 tgatacgctt ggtgaagact ttaacgatgt ctttgtcagc tgccgggata acctggtcga
    27721 acatttcaac cacgtcaatc tgtgaaccca gcgcgtggta tacggtgccc atttccagac
    27781 cgatgatacc gccacccatt accagcaggc gttctggtac ttctttcagt tccagcgcgt
    27841 cagtggagtc ccagatacgc ggatcttcat gcggaataaa cggcagttgg atcgggcgag
    27901 aacccgctgc aatgatcgcg ttgtcgaagt tgatcacggt tttaccgttc tcaccttcaa
    27961 cttccagggt gttagccccg gtaaatttac ccagaccgtt gaccactttg actttgcggc
    28021 ctttcgccat accagccaga ccaccggtca gctgattgat cactttctct ttccaggtac
    28081 gaatcttgtc aatatcggtt ttcggttcgc cgaagacgat accgtgttca gccagcgctt
    28141 tggcttcttc gataactttt gctacgtgca gcagtgcttt agaagggata cagccgacgt
    28201 tcaggcaaac accgccaagg gtgttgtaac gttctacgat tacggtttcc agacctaaat
    28261 cagcgcaacg gaaggccgcg gagtaacctg cggggcctgc cccaagtacc acgacctgag
    28321 ttttgatttc agtactcatc atgacctcta tatatttatc tccggcgggt ctgacgtttt
    28381 ttttatgacg cccattatcc accgggtcgt tctatccgtc ggtattttac aaaattgtta
    28441 acaattttta aacaacaaac ggcaaccgat ttgtctattc gctaataacc tcaatacatt
    28501 catgagatta ccagaaaaaa gccggccgtt gggccggctc ttttacttac atcaccagac
    28561 ggcgaatgtc agacagcgtg ttgttaatga tggtaatgaa acgggcacca tcagcaccgt
    28621 cgatcacgcg gtggtcgaag gagagagaaa tcggcagcat cagacgcggc acgaactctt
    28681 taccattcca caccggctcc atcgcggact tggaaacgcc gaggatagcc acttccggcg
    28741 cgttcacaat cggcgcgaag tgggtagtac ccaggccgcc gatgctggag atggtgaagc
    28801 aaccgccctg catttcgccc gcagtcagct taccgtcacg cgctttctta gaaatagtca
    28861 tcagctcgcg agacagctcg atgatgcctt tcttgttgac gtctttgaat accggaacaa
    28921 ccagaccgtt cggggtatcc accgccacac cgatgttgat gtatttcttc agggtcagac
    28981 gctgaccgtc ttccgacagc gaactattga agcgaggcat ctgctcaaga gctgcagcaa
    29041 cggctttcat gatgaagaca accggggtga tcttcacatc cagcttacgt ttcgccgctt
    29101 cttcgttctg ctgtttacgg aacgcttcca gctcggtgat atcggttttg tcgaagtgag
    29161 taacatgcgg gatcatcacc cagttacggc tcaggttcgc accagaaatt ttctggatac
    29221 ggcccagttc cacttcttcg atttcaccaa acttgctgaa gtccaccttc ggccacggca
    29281 gcatgcctgg gataccgccg ccagtcgccg ccggagctgc ttctgcacgt ttgatagctt
    29341 ctttcacgta agcctgaacg tcttcgcgca ggatacgacc tttacggcca gtgcccttca
    29401 ctttcgccag gttaacgcca aactcgcgtg ccagacggcg gatcagcgga gtcgcgtgaa
    29461 cgtaagcgtc gttttcagca aattcagatt tgccttcggc tttcgcagcc ggagctgctg
    29521 ccggagcttc agcttttgct gccggagccg gcgctgccgc ttcctgtttc gccggagccg
    29581 ccgcaggcgc tgcgccttca acttcgaaga tcataatcag cgagccagtt ttcactttat
    29641 cgccaacgtt gactttcagt tccttcacga cgcctgcaaa cggagccgga acttccatag
    29701 aagctttgtc gccttctacg gtaatcagtg actgttcagc ggcaactttg tcgcccactt
    29761 tcaccatcac ttcggtcact tcaacttcgt caccgccgat atccggaacg ttaacttctt
    29821 tcacgccagc cgctggtgca ggggccgctg ccggagccgc ttcctgttta gccgccggag
    29881 ctgccgcgcc tgcttcaccc gcgacttcga agaccataat cagcgagccg gtagacactt
    29941 tgtcacccac gttcactttg atctctttca cggtgccagc aaacggagcc ggaacctcca
    30001 tagaagcttt atcgccttct acggtgatca gcgactgttc agcttcaact ttatcgccaa
    30061 ctttcaccag gatttcggtc acttcaactt cgtcgctgcc gatatccgga acgttaacgt
    30121 cttttgccgc cgcagccgcg ggtgctgctg ccggagctgc ttctttcttc tcttctgcct
    30181 gagcaggtgc agcgtctgct gcaccgtcgg cggaatcgaa aatcataatc agtgcgccgg
    30241 tctgggtttt atcgccaaca gagactttga tctctttaac gatacccgcc tgcggagacg
    30301 gaacttccat agaggctttg tcgccttcta cggtgatcag cgactgttcg gcttcaactt
    30361 tgtcgcccac tttgaccagg atctcggtga tttcaacttc atcagccccg atgtccggta
    30421 ctttgatttc gatagccatt attcttttac ctcttacgcc agacgcgggt taactttatc
    30481 tgcatcgatg ttgaatttgg cgattgcgtc agcaaccact ttcttatcga tttcgccacg
    30541 tttagccagt tcgcccagcg ccgcaaccac gacataagaa gcatcaactt cgaagtggtg
    30601 acgcaggttc tcacggctgt cggaacgacc gaagccatca gtacccagta cgcggtagtc
    30661 gtcagccggt acgtaagtac ggacctgctc agcgaacagt ttcatatagt cggtagatgc
    30721 cactgccgga gcgtcgttca tcacctgagc gatatacgga acgcgcggag tttccagcgg
    30781 atgaagcatg ttccagcgtt cacaatcctg accatcacgc gccagctcgg tgaaggaggt
    30841 cacgctataa acgtcagaac ctacgccgta atctttcgcc aggatctctg ccgcttcacg
    30901 gacgtgacgc aggatagaac cggagcccag cagctgaact ttacctttgc taccttcaat
    30961 ggtttcgagt ttgtagatac ctttacggat accttcctca gcaccttccg gcattgccgg
    31021 catgtggtag ttttcgttca gcgtagtgat gtagtagtaa acgttctctt gtttttcacc
    31081 gtacatacgc tccagaccgt catgcatgat gacagcaact tcgtaagcgt aagccgggtc
    31141 gtaagagata cagttcggga tagtcagcga ctgaatgtgg ctgtgaccat cttcgtgctg
    31201 cagaccttca ccgttcaggg tggtacgacc ggaagtaccg ccgatcagga agccacgcgc
    31261 ttgctggtcg ccagccgccc agcacaaatc gccgatacgc tggaagccga acatcgagta
    31321 atagatgtag aacgggatca tcggcagatt gttggtgctg taagaggtcg ccgctgccag
    31381 ccaggaacaa cctgcgccca gctcgttgat cccttcctgc agaatctgac ctttctcgtc
    31441 ttctttatag taagcaacct gctcgcggtc ctgcggggtg tactgctgac cgttcgggct
    31501 gtaaatacca atctgacgga acagaccttc cataccgaaa gtacgcgctt cgtcggcgat
    31561 gatcggtacc agacgatctt tgatcgactt gttcttcagc attacgttca gggcacgaac
    31621 gaaagcgata gtggtagaga tctctttgct ctgctcttcc agcagcgcgc cgaagtcttg
    31681 caggctcggc agctcaagct tctcggtgaa gttcggctga cggcttggca gataaccgtg
    31741 cagtttctga cgctgtgcgt gcagataggt atgctcttca gaaccttccg ggaaggtgat
    31801 gtacggcagt ttttcgatat ctgcatcaga caccggcaca ttgaaacggt cgcggatgtg
    31861 acgcacgccg tccatgttca ttttcttaac ctggtgcgcg atgtttttac cttcagccgc
    31921 gtcgcccatg ccgtaacctt taatggtatg agcaaggatt actgtcgctt tgcctttggt
    31981 ttcctgcgct ttcttgaatg cagcgtagat tttcttcgga tcgtgaccac cacggttcag
    32041 tgcccagatc tgctcgtcag tccagtctgc aaccagtgct gcggtttcag gatatttacc
    32101 gaagaagtgt tcacgaacgt acgcaccatc tttcgatttg aaggtttggt agtcgccgtc
    32161 aacggtttcg ttcatcagct ggatcagttt accgctggta tctttacgca gcagttcatc
    32221 ccaacggcta ccccacatca ctttgatcac gttccagcca gcaccttcga agatgccttc
    32281 cagttcgttg atgatcttgc cgttaccggt gaccgggccg tcaagacgct gcaggttaca
    32341 gttgataacg aagaccaggt tatccagttt ttcacgggta gcgatggtga tcgcaccttt
    32401 ggattccggt tcgtccattt caccgtcgcc gaggaacgcg taaacggtct gtttagaggt
    32461 atctttcagg ccacggtgtt ccagatattt caggaattta gcctggtaaa tagcaccaat
    32521 cggccccaga cccatagata cggtcgggaa ctgccagaat tccggcatca gtttcgggtg
    32581 cggataggaa gagaggccat tgccgtgaac ttcctgacgg aagttatcca gctgctcctg
    32641 agtcagacga ccttccagga aagcacgagc gtacacgccc ggggagatgt ggccctggaa
    32701 gtaaaccagg tcgccgccat cctgctcgtt gcgtgcacgg aagaagtggt taaagcacac
    32761 atcataaatg gttgcggaag actggaagga cgccatatgg ccgcccagtt cgaggtcttt
    32821 tttcgacgca cgcagcaccg tcatgatggc gttccagcgg atagctgaac gaatacggcg
    32881 ttccagttcc agattacccg gatactccgg ttgttcttca acggggatgg tgttgatgta
    32941 gttgctgata cctgtgcctg cggctacgtt tacaccgcct ttgcgggctt cagcaagcag
    33001 ttggtcgatc agatactgag cacgctcaac accttcttca cggatgaccg attcgatcgc
    33061 ctggagccag tcgcgagttt cgatcggatc cacgtcattt gggaaacgtt ctgacatggg
    33121 ttattcctta tctatctaat aacgttgagt tttctggaac ctgtcccatt gaactctcgc
    33181 cggaaagctc aataagacag gttctacgtt tagttgccgc gcttttatat gcgcttgatt
    33241 tacaacatct tctggataat ttttaccaga aaaatcacta attctttcgt tgctccagac
    33301 gacgcagaga acgctcacgg cggctctctt cacgacttct gtcgagcaaa atttcttcga
    33361 taaaggccag atggcgatgc gatgcttcgc gcgcttcttc cggcttaccg gccataatcg
    33421 cttcaaatat gcgggtgcgg tgactactca ccagcggcag catctcgcga cgcgaataga
    33481 gcaattcgaa gttctggcgg acattctggg ccaacatcgg ctccatacac cttagcagat
    33541 gaagcagaac cacattgtgg gccgcttcgg tgacggcaat ctgatactgg agtacggcgt
    33601 ttgattccgc gtccagatcg ccagactgct gcgccagctc tatggcgtgg tggagttcac
    33661 ggatgcgttc cttgtcttca tcggtactac gcagcgcggc gtaataagcg gcgatacctt
    33721 ccagggcgtg tcgtgtttcg agcaagtcat actgtgactc aggatggtcg gagagcagct
    33781 ccaccagcgg atcgctgaag ctttgccata ggctgctctg gacaaaagtg ccgccaccct
    33841 ggcgacgaag caacaagccc ttcgcttcga gacgttgaat cgcctcacgc aaggagggac
    33901 gggagacgtc aaactgtttt gccagttcgc gttccggtgg gagtttttcg cccgggcgga
    33961 gagtgccttc gaggatcaaa aactccagtt gctgctcaat cacatcggag agttttggtt
    34021 ggcggatttt gctgtaggcc atgagttcct gtcttaagcc acttgccaag tcaattggtc
    34081 ttaccaattt catgtctgtg acgctaaagt aacaaagtat tcaccttatg tccatacagg
    34141 ttttgattga aatcatgaaa ctgtgcacat tttaacaact tgacatatat aacgtttcaa
    34201 agttgtaact atgcacaaat gtagacttta cgtaggaaag gagtttttta acgattatta
    34261 aactataaaa atccgaattg aaccgattca cttaccaatt ttgtgatttt taattcaatt
    34321 aaaacgaatt taaattcatt ctacatattg agaggggttg aggctgagct ttacaaacgg
    34381 tttcttttta agcaactcat cttcaaccat gcataaagcg ggtgcattcg ctgccgcata
    34441 ccattattct tgatctgacg gaagtctttt tgtaacaatt caaacttctt tgatgtaaac
    34501 aaattaatac aacaaacgga attgcaaact tacacacgca tcactgcgta gatcaaaaaa
    34561 acaaccaccg cacgaggttt catgatggaa ggtcaacagc acggcgagca gctaaagcgc
    34621 ggccttaaaa accgccatat tcagcttatc gcgctgggtg gcgcgatagg gaccgggtta
    34681 ttcctgggta gcgcctccgt aatacagtcc gcagggccag ggattatcct gggttacgcc
    34741 attgctggtt ttatcgcctt tctgatcatg cgtcagctgg gtgaaatggt ggtcgaagaa
    34801 cctgtcgcag gctcctttag ccactttgct tataaatact ggggcagttt tgccggtttc
    34861 gcctctggct ggaactactg ggtactgtac gttttagttg ccatggctga gctgactgcc
    34921 gtgggtaaat acattcagtt ctggtatccg gaaatcccca cctgggtttc tgccgccgta
    34981 ttctttgtgg tgattaacgc catcaacctg accaacgtta aagtgtttgg cgagatggag
    35041 ttctggtttg ccattatcaa agttatcgcg gtggtagcga tgatcatctt cggcggctgg
    35101 ctgctattca gtggcaacgg cggcccgcag gcgaccgtta gcaacctgtg ggatcagggt
    35161 ggtttcctgc cgcacggctt caccgggctg gtgatgatga tggcgattat catgttctcg
    35221 ttcggtggtc tggaactggt ggggatcacc gcagcagaag ctgataaccc ggagcaaagt
    35281 ataccgaaag caactaacca ggttatctac cgcatcctga ttttctatat tggttcgtta
    35341 gccgttctgc tctcactgat gccgtggacc cgcgttaccg ccgataccag tccgtttgtg
    35401 ctgatcttcc acgagttagg cgataccttt gtggcgaatg cgctgaacat cgtggtactg
    35461 actgcggcgc tctccgtgta caacagctgc gtatattgca acagccgtat gctgtttggt
    35521 ctggcacaac agggtaatgc gccaaaagcg ctggcgtctg tcgataaacg tggtgtacca
    35581 gtaaatacca ttctggtgtc tgcactggta acggcgttgt gcgtactgat taactacctt
    35641 gccccagagt ccgctttcgg actgttaatg gcgctggtgg tatctgcact ggtaatcaac
    35701 tgggcgatga ttagcctggc gcatatgaaa ttccgtcgcg ccaagcagga acaaggcgtg
    35761 gtaactcgct tccctgctct gctttatccg ctgggtaact ggatctgcct gctgtttatg
    35821 gcggcggtac tggtgattat gctgatgacc ccaggaatgg cgatttcggt atacctgatc
    35881 ccggtatggc tgatcgtgtt aggtatcggc tatctgttta aagagaaaac cgccaaagcc
    35941 gtaaaagcgc attaatctct ctacgccctc acccgtacag ggtgagggca ataatcttta
    36001 caccaacgcc ccgtaaatcg tcagtagtgc aatcaccacc acgaccacga acgaggtttt
    36061 cttcgccatt gaaaccgctg ccttcggcgt ctccacctta tcgacatgcg gttcacgcgc
    36121 cagagagaac tgcgccagac gcgttaacac ctgatactgc gaagtatgga aatcacccag
    36181 cgaagcaaac caggccggta acgctttctc accatgaccg atcaaggcat ataccacacc
    36241 cgcaagacga accggcaccc aatccagtac atgaagcacg gcatcaatgc cggactgtaa
    36301 acgatgatgc ggcgtctgat atcgcgccag ccagtattgc catgcacgca aaaacgcata
    36361 ccccatcagc gtaacgggtc cccaggttcc ccccacaatc agccagaaca gcggtgcaag
    36421 ataaaaacga aagttaatcc acagcaatgc attttgcagc tcacgcaaat actcacgttc
    36481 gtcgcagcct gccgggacgc cgtgaatcat ggtgagttcg ccagccatcg tggcacgggc
    36541 atggctatca ttacgtgaag cagctgtcag ataagcatga taatgaagac gaactttacc
    36601 tgcgccaata cacagcaaac caatcagcag ccacaccagt agcgtgggaa cgttgaacaa
    36661 tactccctgc aatgcgcgta acagtaaaaa agtcacgccc atcgcaataa tggtcatgcc
    36721 taacgtgcgc ccgagagaaa aatgtttcac ccgccgaaag aacgcttcaa gacgatgatc
    36781 aagctgccag tgctcgccca acttaaacag gcgctcgaaa attaacacca gtaaggttgt
    36841 aaatagcgtc atgttgtctc cttgctgacc agcacacgaa accgtgccca atcaaatgca
    36901 ggaccgggat cggttttccg atccggcgca atatcacaat ggcccgtcat gtttttagcg
    36961 atatccggat agcaatcaat cagtgcccgc gtaaccgccg caagctgttg atactgcgca
    37021 tcggtatacg ccagcgtatc ggtgccttca agctcaatcc caatagaaaa atcattgcag
    37081 cgttcgcgcc cctgatactg agagactccc gcatgccatg cacgtttatc gaaaggaaca
    37141 tactggacta tttcaccatc acggcgaatc aaacagtgag cggagacgcg caaatgggcg
    37201 atctcagcaa agaaaggatg tgcctgcgga tcaatagttc cagtgaataa tgcgtcgatc
    37261 cacggaccgc caaactcgcc tggcggcagg ctaatattgt gcaccaccag cagggtgggt
    37321 gtttcgtcat ccgggcggca atcgtaatgt ggtgagggaa cgcggcgcgc gccaaccagc
    37381 cacccctgtt ctaacaacat gcaggatctc cttatatgtg gtgctaatac ccggttcaga
    37441 gtagcatgtt tctaccttat gattcgttag ctatctggag ttttaacatg ccgcctcgcc
    37501 gctataaccc tgacacccga cgtgacgagc tgctggaacg cattaatctc gatatccccg
    37561 gcgcggtggc ccaggcgctg cgggaagatt taggcggaac agtcgatgcc aacaatgata
    37621 ttacggcaaa acttttaccg gaaaattctc gctctcatgc cacggtgatc acccgcgaga
    37681 atggcgtctt ttgcggcaaa cgctgggttg aagaggtgtt tattcaactg gcaggcgacg
    37741 atgtcaccat aatctggcat gtggatgacg gcgatgtcat caatgccaat caatccttgt
    37801 tcgaacttga aggcccatcc cgcgtgctgt taacgggcga acgcactgcg cttaattttg
    37861 tgcaaaccct ttcaggagtt gccagtaagg tacgccacta tgtcgaattg ctggaaggca
    37921 ccaacacgca gttgttggat acgcgcaaaa ccttacccgg cctgcgttca gctctgaaat
    37981 acgcggtact ttgcggcggc ggagcgaatc accgtctggg gctttctgat gccttcctga
    38041 tcaaagaaaa ccatattatt gcctccggct cagtgcgcca ggcggtcgaa aaagcgtcct
    38101 ggctgcaccc ggatgcgcca gtagaagtcg aagtagagaa tctggaagaa cttgatgaag
    38161 ccctgaaagc aggagccgat atcatcatgc tggataactt cgaaacagaa cagatgcgcg
    38221 aagccgtcaa acgcaccaac ggcaaggcgc tactggaagt gtctggcaac gtcactgaca
    38281 aaacactgcg tgaatttgcc gaaacgggcg tggactttat ctccgtcggt gcgctaacta
    38341 aacacgtaca agcactcgac ctttcaatgc gttttcgcta atagttgaca gatttatccg
    38401 ctccatcgcg ggcggataat ctccccttcc ccaactttct tcgtaacgcc tcgcaaattt
    38461 cttgtctttc gctgcaacga aatagaaaac ctcggaatct gctttcccgt ttcgtttttt
    38521 gcccctcgcc tgtgctgatg gcgtttgcca aagtagcgcc aaccaaatca aggagcgaaa
    38581 cagatggaca agcaacgcgg ttttacactt atcgaactga tggtggttat tggcatcatt
    38641 gccattttaa gcgccattgg tattcccgct tatcaaaact acctgcgcaa agccgcactc
    38701 accgacatgc tacaaacctt tgtgccttac cgtaccgccg tagagttgtg cgcgctggaa
    38761 catggtggat tagatacctg cgacggtggc agcaatggca ttccctcgcc taccaccacc
    38821 cactatgttt cagccatgag tgtggcaaaa ggcgtggtgt cgctgaccgg gcaagaaagt
    38881 ctcaatggac taagcgtcgt catgacaccg ggttgggata acgcaaacgg cgtcaccggc
    38941 tggacgcgca actgcaatat tcaaagtgac agcgcattgc agcaagcctg cgaagatgtc
    39001 ttccgctttg atgacgccaa ctaaggagcg gcaatgaata ttccacagct cacggccctg
    39061 tgtctgcgtt atcagggagt cttgctggat gccagcgaag aagtggttca tgttgcggtg
    39121 gtcgatgccc cctcacatga gttgctggac gcattgcatt tcgctaccac caaacgtatt
    39181 gagatcacct gctggacgcg ccaacaaatg gaaggtcacg ccagtcgcac acaacagaca
    39241 ttgcccgtag ctgttcagga gaagcatcag cccaaagcag agttgctagc tcgaacgtta
    39301 caatctgcgc tggaacaacg cgcgtctgat attcatatcg aaccagcgga caatgcctac
    39361 cgcatccgct tgcgtatcga cggcgtattg catcctttac cggatgtttc accggatgcc
    39421 ggagtcgcat taaccgccag attaaaagtg ctgggaaacc tggatattgc ggaacatcgc
    39481 ctgccgcagg acgggcaatt cactgtcgaa ctggcaggaa acgccgtctc atttcgtatt
    39541 gcgaccttac catgtcgggg tggtgaaaag gtggtattaa ggttgttaca gcaggtgggt
    39601 caggcactgg atgtcaacac gcttggaatg cagccgttac aactggcgga ctttgctcat
    39661 gccttgcaac aaccacaggg actggtgctg gtaactggcc ctaccggcag cggcaaaacg
    39721 gtcacgcttt atagtgccct gcaaacgctg aataccgctg acattaatat ttgtagcgtc
    39781 gaagatccgg ttgagatccc catagccgga ctaaaccaga cgcaaatcca tccgcgtgcc
    39841 gggctcacct ttcagggcgt tttgcgtgcg ttattgcgcc aggatcctga cgtcatcatg
    39901 atcggagaga tccgcgatgg cgaaacagca gagatcgcta ttaaagcggc gcaaactggt
    39961 cacctggtgt tgtctaccct acacactaat tccacctgcg aaacgctggt acgtttacag
    40021 caaatgggag tcgcccgctg gatgctctca tcagcgctta cgctggtaat agcccagcgt
    40081 ctggtacgta aactttgccc acattgtcgc cagcagcaag gggagcccat ccatattcca
    40141 gtcaatgtat ggccgtcgcc gctgccccac tggcaggcac ccggttgtgt acattgctac
    40201 cacggttttt atggtcgtac ggccttattt gaagttctgc ccataacgcc ggtcattcgt
    40261 cagcttattt ccgctaatac cgacgttgaa tcgctggaaa cgcacgcacg acaggcgggt
    40321 atgtgtacgc tttttgaaaa cggctgcctg gccgtggagc aaggcttaac cacctttgaa
    40381 gagttaatcc gcgtactggg gatgccgcat ggcgagtaag caactctggc gctggcatgg
    40441 cataaccggc gacggcaatg cgcaagatgg gatgctatgg gcagagagcc gtgctttgct
    40501 gctcatggca ctacagcaac agatggttac cccacttagc ctgaagcgaa tcgccatcaa
    40561 ttctgcgcag tggcgaggag ataaaagcgc ggaagtcatt catcaactgg cgacgctact
    40621 caaagccggg ttaacgcttt ctgaagggct ggcactgctg gcggaacagc atcccagtaa
    40681 gcaatggcaa gcgttgctgc aatcgctggc gcacgatctc gaacagggca ttgctttttc
    40741 caatgcctta ttaccctggt cagaggtatt tccgccactc tatcaggcga tgatccgcac
    40801 gggtgaactg accggtaagc tggatgaatg ctgctttgaa ctggcgcgtc agcaaaaagc
    40861 ccagcgtcag ttgaccgaca aagtgaaatc agcgttacgt tatcccatca tcattttagc
    40921 gatggcaatc atggtggttg tggcaattct gcattttgtt ctgccggagt ttgccgctat
    40981 ctataagacc ttcaacaccc cactaccggc actaacgcag gggatcatga cgctggcaga
    41041 ctttagtggc gaatggagct ggctgctggt gttgttcggc tttctgctgg cgatagccaa
    41101 taagttgctg atgcgccgcc ctacctggct tatcgcgcgg cagaaattgt tgttacgaat
    41161 cccgattatg ggttcactga tgaggggaca aaaactcacg cagatcttta cgattctggc
    41221 gctgacacaa agtgcaggca ttactttttt acagggcgta gagagcgtca gagaaacaat
    41281 gcgctgcccg tactgggtgc aacttctgac acaaatccag cacgatatca gtaacggtca
    41341 tcccatctgg ctggcgctaa aaaatgccgg tgagtttagc ccgctctgtt tgcaattagt
    41401 gagaacagga gaggcatccg gctctctgga tctcatgtta gacaacctcg cccatcatca
    41461 tcgggaaaac acaatggcgc tggcggataa cctcgcagcc ttactggaac cggcgttgct
    41521 gatcataacg ggaggaatta ttggtacgct ggtggtggca atgtatctgc caattttcca
    41581 tttaggcgat gcgatgagtg ggatgggata acgctggcgt gttgctccac gccagcgttg
    41641 ggagattaca ggttgttgaa gatgcggttt tcttgttcct gcacacgaat aaacgtggtg
    41701 cgcttggtca gctctttcag gcgtgaagcc ccaacgtatg tacaagctga acgcaggccg
    41761 cccaaaatat ctcgcgcggt attttcaacc gggcctcgca gcggcagctt aacggtttta
    41821 ccttctgctg cgcgatattc cgcaacgccg ccaacgtgac gtttcatcgc agactcggag
    41881 ctcatgccgt agaacagcat aaatttctcg ccgttctcct caacgatgcg accgccgctc
    41941 tcttcgtagc ccgccagcat gccgccaagc atgacgaaat cggcaccgcc gccaaaggct
    42001 ttcgccacat cgcccggcgt ggtgcagcca ccatcgctga cgatcattcc gcccagaccg
    42061 tgcgcagcat cggcacattc gattaccgca gaaagttgcg gataaccgac gcctgttttg
    42121 acgcgagttg tacaaacaga acctgggcca atgccaactt taacgatatc ggcacctgag
    42181 aggataagct cctcacacat ttcaccagtc actacgttac cagcacaaat ggttttggtc
    42241 ggccacgctt cacgcgcttt cgcaacgaac tgcacgaagt gttcggaata accattcgcc
    42301 acgtcaatac aaacgaagtt taatgccggg ttcaggtcga gaatctgttt agttttttcg
    42361 aaatccgcat cagacgtacc ggtagaaacc atcacatgtt tcagcacatc agcggaagaa
    42421 ttgttgataa acgcttgcca ctcttcgaca gaatagtgtt tatgcacagc agtcaaaata
    42481 tcaaaagaag ccagcgcaga ggccatagaa aatgtgccta cggtgtccat atttgcggcg
    42541 ataatcggca cgccggacca gctctgacct gaatgtttga aggtgaattg acgttccagt
    42601 tcaacatcgg aacggctttt aagagtggag cgtttagggc ggatgagaac gtctttaaaa
    42661 cctaacttca gatcttcttc aatacgcatg tgcggattcc tggggttaat ggctataagg
    42721 gtaagagcgc aactccagtg acgctatcat acgcactaat caatgcggcg caagactgcg
    42781 aaattgtctt tttttacgct acaatcccat aaatttacct ggtaaatagt gagcgaacgt
    42841 ttcatcaatt tcgctttcgt gcactcattt ttaacgtttt gataatcatg atgaattccc
    42901 gggaatataa attatgaggt atatagttgc cttaacggga ggcattggca gtggcaagag
    42961 taccgttgcc aatgcgtttg ctgatctcgg aattaacgtc attgatgccg atattattgc
    43021 gcgtcaggtg gttgaaccag gtgcacctgc gctacatgcc attgctgatc actttggcgc
    43081 taacatgatt gctgctgatg gaacattgca gcgccgggcc ttgcgcgagc ggatcttcgc
    43141 caacccggaa gagaaaaact ggcttaacgc cctgctgcat ccgctgattc agcaagagac
    43201 gcaacaccag atccagcaag ctacttcccc ctatgtactg tgggttgtgc cattgctggt
    43261 agaaaactca ctgtataaaa aagcgaatcg agtgctggtg gtggatgtca gcccagaaac
    43321 gcaacttaag cgcaccatgc agcgcgatga tgtaactcgc gagcatgtcg aacaaatcct
    43381 tgctgctcag gcaacgcgcg aagcccgcct tgccgtggca gatgacgtca ttgataataa
    43441 cggcgcaccg gatgctatcg catcggatgt tgcccgcctg cacgcacact atttgcagct
    43501 tgcgtcgcag tttgtctcac aggaaaaacc gtaatgcaga cccaggtcct ttttgaacat
    43561 ccactaaatg aaaaaatgcg tacatggctg cgcattgagt ttttgattca gcaactcacc
    43621 gttaatttac ccatcgttga ccacgctggc gcgctgcatt tcttccgtaa tgtcagtgaa
    43681 ttactggatg ttttcgagcg cggcgaagtc cgcactgagc tgttgaaaga acttgaccgg
    43741 cagcaacgta aactccagac ctggattggc gtgcctggcg tggaccagag ccgtattgaa
    43801 gcattaattc agcagttaaa agcggcgggg agcgtattaa tttccgcgcc gcgtatcggg
    43861 caatttctgc gtgaagatcg tttgattgct ctggtgcgtc agcgactgag catcccaggc
    43921 ggctgttgca gctttgattt acctacattg cacatttggc tgcatctacc ccaggcgcag
    43981 cgcgacagcc aggtagaaac ctggattgcc agcctgaacc cgctcaccca ggcacttacc
    44041 atggtgctgg atttaattcg ccagtcggcc cccttccgta aacaaaccag cctgaatggt
    44101 ttttatcagg ataacggtgg cgatgccgac ttgctgcgcc tgaatctgtc gctcgattca
    44161 cagctttatc cgcaaatttc cggacataag agccgttttg ccattcgttt tatgccgctg
    44221 gacactgaaa acggacaggt accggaacgt ctggatttcg aactggcctg ttgctaagga
    44281 gtaaaaatgt cagaaactat tacggtgaat tgcccaacct gcgggaaaac ggtggtgtgg
    44341 ggtgaaatca gcccgtttcg gccattttgc tccaaacgtt gtcagctgat cgacctcgga
    44401 gaatgggctg ctgaagaaaa acgaatcccc agcagtggcg atctttccga aagcgatgac
    44461 tggagcgaag agccaaagca gtgacatttg tctgatgccg cacgtaggcc tgataagacg
    44521 cggacagcgt cgcatcaggc atcttgtgca aatgtcggat gcggcgtgaa cgccttatcc
    44581 gacctacaaa aacaaattta tcaatgagtt agaaatccct gtaggcctga taagacgcgc
    44641 cagcgtcgca tcaggcatct tgtgcgaatg tcggatgcgg cgaaaacgcc ttatctgacc
    44701 tacagacgtt taagcttcgc aattaccggt tcattggctg gcggaaaatc atcggcatta
    44761 agatcgacca gcgccatcca ctcaccgggt tgcccttctt taccccacgg cttcccttcc
    44821 cagcgttcga ccagccagaa ccacagtgtt atatgcctgt ccgggaattc atattccagt
    44881 ttttcaaata gcgaaaaatg ttgtggggta atcccgactt cttcctgaag ttcacgcacc
    44941 accgcctgtt ccggcgtttc acccatttca attttaccgc cgggaaactc cagtttattc
    45001 gccatgtgcg catctgctgc gcgacgcgtt ataaagattt cattgttctc gttgcgaata
    45061 atacctaccg caatttgcag ctttttcatt gtcttaaacc tataaaaaag gcgcagaatc
    45121 ctgcgccttt tacttcaact gttagctttt attgcaggcg accatggcac tgcttgtatt
    45181 ttttaccaga accgcacggg caaggatcgt tacgtcctac tttgcgttca ccggtttgcg
    45241 ccgccagtgc agctgcggct gcagagtcgt catcctgatg gctaagctgc tgcatttgcg
    45301 ctaaacgctc ggcttccata cgacgctgtt gttccagctc ctcaacttct tcaggcatac
    45361 gtacctgaac tttgctcagc gtactgataa cttcatattt caacgactcc agcatcgctg
    45421 caaacatgga gaacgattca cgtttgtatt cctgcttcgg atctttctgt gcatagcctc
    45481 gcaggtggat accctgacgc agatagtcca tcgctgccag gtgctctttc cacagggagt
    45541 caagcgtttg cagcatgacg cctttctcga agtgacgcat catctctgca ccaaccactt
    45601 cttctttacg ctgatacact tcgatggact gcgccagaat gcgctcacgc agcgtctctt
    45661 catgcagttc gggttcttta tccagccact cagcaattgg taaatcgagg tcgaaatcgt
    45721 tcttcagacg ttcctgcagc cccggaatat cccacatttc ttccagcgac tgcggcggaa
    45781 tgtaggcatc aatggtggct ttgaacacat cttcacgaat gctgttgatg gtttcgctca
    45841 catcgctaac atccagcagt tcgttacgct gggagtaaat ggcgcgacgc tgatcgttag
    45901 ccacgtcatc atattccagc agttgcttac gaatgtcgaa gttacgactt tcaactttac
    45961 gctgggcgtt ggcaatcgct ttggtcaccc acgggtgttc aatggcttcg cctggcttca
    46021 tacccagttt acgcatcatg ccggatactc ggtcggaagc aaaaatacgc atcagcgcat
    46081 cttccatcga caggtagaaa cgggaagaac cagcatcccc ctgacgacca gaacgaccgc
    46141 gcaactggtt atcgatacga cgggattcgt gacgctcggt accgatgata tgcaggccac
    46201 ctgctgccag taccgcatcg tgacgtacct gccagtcggc tttaattttt tcaatttgct
    46261 ctgcggtcgg attttccagc gcggcaactt ctgcctgcca gctaccaccg agcacaatat
    46321 ctgtaccacg acccgccatg ttggtcgcga tagtcaccgc agccggataa cctgcctgag
    46381 caacaatcgc cgcttcgttg gcgtggaatt tggcgttcag gacattgtgc ttaataccgg
    46441 ctttggtcag ttcgtttgac accagctccg atttttcgat ggagatagta cccaccagca
    46501 ccggctggcc tttcgcagta cgttctttga tatcttcaat gatcgcctga attttttccg
    46561 cttcagtcat gtagaccagg tccggcaaat ctttacgaat cattggacgg ttggtcggaa
    46621 caacgacggt atccagcttg tagatggagc taaattcgaa agcttcggta tcagcagtac
    46681 cggtcatccc cgccagtttt tcatacagac ggaagtagtt ctggaaggtg atcgaagcca
    46741 gcgtttggtt ttcgttctgg atctgcacac cttctttcgc ttccacagcc tggtgcagac
    46801 catcggacca gcgacggccc tgcatggtac gaccggtgtg ttcgtcaacg atgataactt
    46861 caccatcttt aacgatgtag tcgacgtcgc gggtaaacag cgcatgagcg cgcagcgccg
    46921 ccgttacgtg gtgcatcagc atgatgttgg ccggagagta cagagactcc ccttcatcca
    46981 tgatgccctc tttcaccagc agttcttcaa tcagcaccag accacgttcg gtcaggttca
    47041 cctggcgaga tttttcgtcc accgagaagt ggccttcgcc ttggaaggtt tcggagtctt
    47101 ctttttcctg acggatcagg tgcggaataa ttttattcac gcgtttatac atttccgagc
    47161 tgtcttctgc cgggccggaa atgatcagcg gtgtacgcgc ttcatcgatc aggatggagt
    47221 ccacttcgtc caccagcgca tagtgcagtt tacgctgtac acgttcttca gggctgaacg
    47281 ccatattgtc gcgcaggtag tcaaagccgt attcgttgtt cgtaccgtaa gtgatgtcag
    47341 ctgcgtaagc ttcgcgcttt gccggtgctg gcatgcccgg caggttgata ccgacagtca
    47401 ggccaaggaa ttcaaacagc ggacggttgt tttcggcgtc acgttgcgcc aggtagtcgt
    47461 tgacggtaac tacgtgcacg cctttaccgg ttagtgcgtt caggtaagca ggcagcgttg
    47521 cggtcagggt tttaccttca ccggtacgca tttcggcgat gcagcgttcg ttaagaacca
    47581 taccgccgag taactgaacg tcgaagtgac gcataccaaa gacgcgctta cttgcctcac
    47641 gtaccacggc gaaagcttcc gggatcagat tttccagcac ttcgcctttt tccagacgtg
    47701 cacgaaactc tgcggttttc cctttcagtt cttcgtcgga gagtttttcc atctccggtt
    47761 ccatggcatt gatgatgttg accactttgc gcatccggcg cagggtgcga tcgttacgac
    47821 taccgaaaac tttagttaac aatttgatta gcataataaa atctcaaacg ccccgcgttg
    47881 cggagttaat aaaatgaagt aaaggtttat tgttgttagg tgaggcgttg agggccagca
    47941 cggatgcctt gcgcctggct tatccagacg ggcgtgctga attttgcttg tggggtaaaa
    48001 tgcgcataat caatgcgata acccttttca gacggcgtgc cttcctgggt cagcagcgcg
    48061 ctgagcgtat ccagtaatgc aagatgttgc gcctgaagag gcaaagattc ttcagcaacg
    48121 ggcagtgttt gcggtgccat tgcgaaagaa agatgacgga ttaccgtgcg aatggcatgt
    48181 tgatgccagt aatcaacgga atagttcgaa ttcgggcggc gtgtgttcgc ttccagcaag
    48241 gccaattgac caaagttaac tttggctgaa ggctcgtggt tgcgggttgt cgcttttgcg
    48301 ggcgcgtttg gttcggcggc gttgctgagc gcaggcaaac ctaaactcgc cgcaaccatc
    48361 cctaataaga gatgcggcca gaagtagcgt ttaccaaact gtcgccagcg cgtcagtatt
    48421 ccactcacgt tattgccatc ccgatcgtat tcaaacgtat ttagcgcaca aatcttatca
    48481 ttaaagcctg cagtccacag cacgaatgaa gataagttgt gcggaaaagt gcttaagaaa
    48541 acagcgttcg caccgttttt tatagctctt ataaaattca aaaaaaaacg actggttcac
    48601 ctggccggag agagtgccag atttgccagt cgaattttat acgacagtat aaatgtcgtt
    48661 atgccagtac agctgaaggc gctttgaagg ccaacggcag ttctgcgtcg tcctggaagg
    48721 tcacatattc ccaggcttcc tgtttcgcca ggacagcctg cagcagtttg ttattcagtg
    48781 catgaccgga tttataagcg gtaaatgcac caataatatt gtgaccacac atgaacaagt
    48841 caccgatcgc atcgagcatt ttgtgacgca caaattcgtc ttcaaaacgc aggccgtctt
    48901 cgttcagtac gcgataatcg tcaacaacga tggcacaatc gaagctgccg cccaggcaca
    48961 aaccacggga ctgcagatat tcgatatcac gcatgaaacc gaacgtacgc gcacggctga
    49021 tctggcgcat aaacgcatca gcggagaagt tcatcgcata gcgctggttg ctggaatcaa
    49081 tagccggatg gttaaaatcg atggtgaaat ccagcgaaaa accattgtac ggcttaaatt
    49141 cagcccactt atcgccatct tcgacacgaa cagtctcttt gatgcgaaca aattttttgg
    49201 cgcagttcaa ctcgtcgata ccggcgtcaa gcagcaggta tacaaacgga gcggcgctgc
    49261 cgtccatgat cgggatttcc ggcgcgttaa cttcgataac aatgttatcg atgcccaggc
    49321 ccgcgagagc agcattgagg tgctctacgg ttgaaatccg tacatcatgc tcgttgacca
    49381 gacacgtaca gagcatggta tcacgcacag atttggcatc ggccgggaaa tctaccggtg
    49441 gattcaagtc ggtgcgacga tagatgaccc cggtgttggc cggcgcaggg cgtaacgtca
    49501 gggtgacttt cttgccggta tgtaaaccga cacccgtcgc ctgaacgata cgtttaagtg
    49561 tcctttgttt gatcatcgta ttatctcgcc aaattaccta tccaaccgaa gtgtactata
    49621 cattcggcgg gccagtttag cacaaagagc ctcgaaaccc aaattccagt caattcttaa
    49681 tcagcttgct tacgcaggaa tgctgggata tccagataat ccggctcttt cgcagtttgc
    49741 ggcgcattgt cattcacgac tttagcaact ggcttctgct cctgggtcag cggagccatc
    49801 ccatgctgct ggtagcgatc catcactggc tgctgaacct gcttattggt caccagagtg
    49861 atttcaggac gtttgtccat gccgatacct gtcgcaacaa cagttacgcg cagctcgtca
    49921 ttcatatccg ggtcaagaga agtaccgata accacagtcg cgttgtcgga agcaaatgca
    49981 cggatggtgt tacctaccgt ttcgaactca tccagacgca ggtcgaagcc cgctgtgatg
    50041 ttaaccagca cgccgcgtgc gccagatagg tcgatatctt ccagcagcgg agaagaaata
    50101 gccatttcag cagcttcttc cgcacggtct tcaccgctcg ccacgccaga acccatcatt
    50161 gcgtagccca tttcagacat tacggtgcgt acgtctgcaa agtccacgtt catcaaaccc
    50221 ggacgagtaa tcagttcggc gatgccctga acagcgcctt tcagtacatc gttcgctgcg
    50281 ccaaacgcat ccagcaggga gataccgcgg cccagaactt tcagcagttt gtcgttcggg
    50341 atagtgatca gagagtccac atgcttggac agttcagtga tcccctgctc cgcgaatgcc
    50401 atacgcttct tgccttcaaa gttgaaaggc ttagtgacga cagcaacggt caggataccc
    50461 aaatcttttg ccacttcagc gacgactggt gctgcacctg taccggtacc accacccata
    50521 cccgcagcaa taaagaccat gtctgcacct tccagcgccg cacgcagtgc atcgcgatcc
    50581 tcatcagccg cattgcggcc aacctctgga ttagcgccag cgcccagtcc tttggtgata
    50641 ccgctaccga tttgaatcgt ctgtccaacc gctgttttac gcagcgcttg tgcatcggta
    50701 tttaccgcga agaattcaac accttcaata cgctcgcgca ccatgtgttc aacagcatta
    50761 ccgccgccgc cgccgacgcc gatgacttta atcaccgcgt cattggtaag ttccattggt
    50821 tcaaacatag tttctctccg atttgtgcct gtcgcctgag gccgtaatca tcgtcggcct
    50881 cataaaaatt aaaactcttt tcgcagccaa ctattgagtc gcttgatcca cgagccaact
    50941 gatgctgtaa cacgtttttc tacttcagct tcaccgttaa gatgtgactc tttcccatag
    51001 tgaagcaatc ccaccgccgt cgaataatac ggctcctgag cataatccgt taaaccggta
    51061 atgttcagcg gcgcgccgat acgcacttgc gtatgaaaca cgcgctgagc acaggctgca
    51121 agaccttcga tctgcgctgc gccaccggtt aatacaatgc ctgccgccag gtgatgttta
    51181 accccttgtt ggcgaagctt ttcctgcaac tgcaatatct cttcgttgac caggttgagc
    51241 agctcggtat agcgcggctc gatcacctct gccagggtct gacgttgcag actccgcggc
    51301 ggacgaccac ctacgctcgg cacttccacg ctctcatctt ttccaacgat ggaacccagc
    51361 gcacaaccgt ggcgaacttt aatcgcttcg gcgtcgcttg gcggcgtgcc aaaggcgtaa
    51421 gcgatatcac tggtcacgac attgccagca taaggaatta ccttagtgtg gcgcaatgcc
    51481 ccaccggtat aaacggcgat atccattgta ccaccaccga tatcgacgac gcagacaccc
    51541 agttcacgtt catcttccgt caataccgaa taacttgatg ccagtccggc aaatatcagt
    51601 tggtcaactt tcagcccaca acgttcaacc gctttgacga tgtttttcgc catatcgttg
    51661 tgacatgtga tcaggtgcac ttttgcctgc atccgcacgc ccgaaagtcc taccggattc
    51721 ttgatccctt cctgatagtc aatcgcatac tcttgcggga tcacatgcag cacacgatgc
    51781 tcatcgcgca cacgcaccga tttcgcggta tggacgacgt tttccacatc ttcttgcgtc
    51841 acttcttctt cagaaatagg caccatacca atttcattct ggcagctgat gtgcttacca
    51901 gaaagcgcca gatataccga agagatctga caatctgcca tcaattctgc ctggtcaatg
    51961 gcgcgttgta cgcacttgac cacggattcg aggtcgttca ccccgccttt atccatacca
    52021 cgcgacgggc agctgcccac gccaatgata ttgaccatac cgtcgggcag aacttcccct
    52081 actaaagcgg caaccttcgc ggtaccaatc tccagtccta ctaccagttt tctgtccgtc
    52141 gccttgatca ttgttgttct gcctgtgcct gattttgttg ctgagtagat tcctctggcg
    52201 gcaagggcgc ccagcctact gccgctccag agtcataacg caaatcaacg tagctaatcc
    52261 gtttgccatc ggtttgcgcc tgctgctgta aaaccggata aagttctaca aagcgagcca
    52321 aacgtttcat cgtatcgccc cggccaagat tgagcttaat atcgttattc agcgtcaact
    52381 gccaggaacg ccgcgcggtc atcgccgctt ccttcagagt aaatctgtcc tttgccagca
    52441 tctgccccat ttcgcgatag ccctgcaaca cttcattggc gctgccttcc gggccataca
    52501 gcattggaag cacctgcttg ctggtgcgtt ctggcggcac gctgaaggta tttccttccg
    52561 cgtctaccat atgttgatca ttccaccgcg caatcggcac atattcaacc agatgaatct
    52621 tcaattcatc aggccactgc tttctgacgc tcacctgctt aatccacggc aggcgttgtt
    52681 ctatttgcgt ctggatgatg ttgacatcct gggtcataaa ggtacccggc tcacccaatg
    52741 ccaggatcga ctgccggata tcgtcattac gtgtgtaatg gcgttcaccg gtcaacacca
    52801 gctttgagag cggcaggcgt tgcgcatctt ccatccagcc caacacgacc cagccgctca
    52861 ccaacactgt cgttaaaacg gtcagcagga aaaggatccc cgccagacgc gttccattat
    52921 tgcggcgaga agaaacctct tcttcgctgt ttcgcgtgtt cagagcagcc tgcgacatat
    52981 tagtccgcca gttccagaat tcgtactacc aactgcgaga agctcatacc tgcctgacgt
    53041 gccgccatcg gcaccaggct gtggctggtc atacccggtg aggtattggc ttccagcaga
    53101 taaaactgtc catcgctgtc cagcataacg tcaatacgtc cccatccttt gcaacctaac
    53161 gtcgtccatg ctttcagcac taatgcctgc aaattggcct cttgtgacgc ttccagacct
    53221 gcggggcaga aatactgtgt ctcatcagag agatacttcg cctcataatc atagaaggtt
    53281 ccggacggtt gaatacgtat tgacggtaaa atttcttcac cgagtatcgc aaccgtgaac
    53341 tccggcccac ttagccattt ttcaatcaat acttcttcat cgtgctgaaa tgccaatctt
    53401 aatgcatctt gtagagcatt ttctgctact acttttgaca ttcccacact ggaaccttcg
    53461 cggctcggct taacgataac cggcaaaccc agagcagaaa tttctgctaa ctgcttatcg
    53521 ctcaggcctt tttcaaactc tgcgcgggtt aacgctaccc acggcgcgac cggtaaaccg
    53581 gcaccttgcc atagaagttt gctgcgtagt ttatccattg aaagcgcaga tgccatcact
    53641 ccgcttccgg tataaggcaa gcccatcagc tcgagcatcc cctgcagcgt accatcttca
    53701 ccgccgcgac cgtgtagcgc gataaacact ttctgaaagc ccatcgactt cagttgcgtc
    53761 acgtcgactt ctttcgggtc gacaggatac gcgtcaatac cgccttcacg cagtccggct
    53821 aacaccgctg cgccagaatt cagagaaact tcccgctcag cggaggtccc acccaacagg
    53881 accgcgattt tatcagtcat gttgttcttc ctccggagtt tgcggcttca gtttgatttc
    53941 agctaaagaa cgagcaattt ttccaatatt accagccccc tgaacgagaa tcaggtcgtt
    54001 accggttaat accggtgcca gcatctcggc tacccgcgcc ggatccggca ccagaatggg
    54061 atcaattttc ccacgtccac gaattgtgcg acacagcgaa cggctgtccg ctcccggaat
    54121 tggcgcttcg ccagccggat acacttccag catcaacagg gtatcaacct gcgtcagcac
    54181 attggcgaaa tcatcataca ggtcgcgcgt acgggtaaaa cggtgcggct gaaacagcat
    54241 taccaggttt ttatccggcc agcctgcgcg cgccgcttta atggtggcgt ccacttcagt
    54301 cgggtggtgg ccgtagtcat cgaccagcat tgccgtaccg cttttaccat tcactggctc
    54361 cagcgggaat tcaccgagga aatcaaaacg gcgaccagtc ccctggaagc tttcaagcgc
    54421 ccgcaaaata gcctcgtcgt caatgccctc ttccgtagca accgcaaccg cagctgcggc
    54481 gttcagcgcg ttatgacgac ctggcgcatt cagggtgacg cgcatcggct ctttgtcctg
    54541 gcgcagcagc gtaaagtgcc cctgcgggcc aatctgctga taatcttcta cacgcacgtc
    54601 ggcatcttcg ctgaagccgt aagtcgtggt ctgacgcccc actcgcggta acaattcgcg
    54661 gatcaccgga tcatcaacac acatcaccgc acgaccgtaa aacggcaggt tgtgcagaaa
    54721 attaataaaa gtctgtttta aattctcaaa gtcgccctgg taggtatcca tgtggtcggc
    54781 ttcgatattg gtgacaatcg ccaccatcgg ttgcagatgc aggaacgatg catcactctc
    54841 atctgcttcg gcaatcaggt accgaccatg ccccaaacgc gcatgaaccc ccgccgcttt
    54901 taccagcccg ccgttaacga aggttgggtc gagccccgct tctgcgtaga tgctggaaac
    54961 catcgcggta gttgtcgttt tgccgtgcgt tcctgcaatg gcgatgccat gacgaaaacg
    55021 cattaactca gccagcattt cggcacgacg gatcaccgga atacgcgctt catgagctgc
    55081 gacaatttcc gggttgtcgg cagaaatcgc gctggaaaca acgaccacgc tggcatcacg
    55141 tacgttttcc gggcgatggt tgaaataaat cgtcgctccc agattcatta actgctgcgt
    55201 gaccggattt ggcgctaaat cggaaccact gatctgataa ccttcattgg ccagaacttc
    55261 ggcaataccg cccataccgg caccaccgat gccgacaaaa tgtatgtgcc gaacgcgacg
    55321 catttcgggc acgatggaac gcagttttgc caattgttgt gtattcattc tttacgccat
    55381 taacttctta aattcatacg atgcaaaagg catcgctaca attacgcccg ggcagcccgg
    55441 ctcacttcat ttgccactcg ctcggtggca tccggaatgg atgcagcgcg ggcgcgttct
    55501 gccatggtta ataaggtttc tcgcgaccac ccggccaggg tgttggcgac agcatccacg
    55561 ctaagctgtg gctgttcgat aattttggct gcgcccgctt tttccagcgg tagcgcattc
    55621 cagtattgct ggcggtcttt atgttgaaac ggcacaaaca acgccggtag tcctgccgcg
    55681 gcgatttcac tcaccgttaa cgcaccggag cggcaaacga cgacatccgc ccacgcatac
    55741 gccgccgcca tatcatcaat aaattccgtc actttatgct gcggttgccc cgcttcggca
    55801 tacgcctgtt caacggattg ttgcgaacct ttgccgctct gatgccagat agtgactgaa
    55861 tcacccagtt tcgcagcaac ctgcggcatt gtctggttaa gaatgcgtgc gccctgagac
    55921 ccccccacta ccagcacacg aaccggacct tcacgtccag ccaaacgttg ctgcggcaac
    55981 ggcagcgcca acacatcggt acgcaccggg ttacccacta cttccgcatt agggaaagca
    56041 cctggaaacg cctgcatcac tttggtggca atcttcgcca gccatttatt ggttaagccc
    56101 gcaataccgt tttgttcatg aagtacaacc ggaatgccta acgaccacgc ggccagacca
    56161 cctggacctg acacatagcc gcccataccg agcaccacgt caggtttgta cgctttcata
    56221 atcgcccgcg cctgacgcca ggcattgaag atacgcagcg gcgcagctat cagtgctttt
    56281 ataccttttc cacgcagacc agagatacga atgaaatcaa tttcgatgcc atgttttggc
    56341 actaagtccg cttccatacg gtcagcagtc cccagccagc gaacttgcca accctgagcc
    56401 attagatggt gcgcaaccgc cagtcccggg aatacatgtc caccggttcc gcctgccatc
    56461 accattaatc gctttccttg accactcatc gtgaacctcg tacaaacgcc tgcgctttct
    56521 ccagacgcgt ttcataatca atacgcaaca gcatcatgat ggctgtcgac ataatcagta
    56581 agctcgaacc accgtaactg atcagcggca atgtcagacc tttggtcggt aacatccccg
    56641 ccgccgcgcc tacgttaacc agcgcctgga agctaaacca gatgccaatg gaacaggcga
    56701 gaaaaccgga aaaacggtgg tcaatttcta acgctttgcg gccaatcgac atcgcacgaa
    56761 aagcgacgaa gaataccatt aaaagtgcca gcaccacacc gacatacccc agttcttcgc
    56821 cgataatggc gaaaataaag tcagtgtgcg cttccggcag atactccagt ttttgtaccg
    56881 agttacctaa accttgcccc caaagttcgc cgcgaccaaa cgccatcagc gattgcgtta
    56941 actgatagcc gctgccaaag ggatcttccc acgggttcca gaatgcggta acacggcgga
    57001 tacggtacgg ttcggcgagt atcagcaaca caaccgctga aatgcccata ccgataatgg
    57061 caatgaactg ccacaatttc gctcccgcca ggaacaacat cgccagcgta gtcacaaaca
    57121 acaccaccac cgtaccaagg tctggctgtg ccagcagtaa cactgccaac accagaatca
    57181 cgcccatcgg tttcaggaag ccgcgcaggt tattacgtac ttcgtcgcct ttacgcacca
    57241 gatagttggc gatatagcaa aacagcgaca gttttgtcag ctccgcaggc tggatacgca
    57301 gcaaaccgag atcgatccaa cgcgatgccc ctttaaccga gctacccact accagaacga
    57361 tcatcagcag gatgatagat ccgagcagca tcgtggcact gtagcgttgc cagaactcca
    57421 tcggcagacg cagcgtaatg atcgccagaa taaacgccaa aatcagatag acaccatcac
    57481 gcttcgcgaa gaagaacgga tcgttggtta agcgttgccc tatgggcatt gacgccgagg
    57541 tcaccatgat aaagccaatc gccgcgaggc cgaaggtcag ccacagtaag gtgcgatcgt
    57601 acatgatcag gctgtcggta tctttttccc gcgagcccat cacccagccc tttagcgccg
    57661 tggagatcca gaccaggata ctgaatcctg gcaggcgcgg cattttcagg cgagggagag
    57721 ataaacgcat caacctaact ccttcgccag acgggcaaac tcattgcctc gttgttcaaa
    57781 gttcttgaac tgatcaaggc tggcacaggc cggggagagt agaaccatat cgcccggctg
    57841 aacacgcgga gccagcaagc gcatcgcctg ttccatagtt tcggtttgtt ctgccacttc
    57901 cgggtgtagc gccgccagct gcgcgccgtc acgaccgaaa caatacagac gtacgttatc
    57961 gccattcagg taacgcgcca gtgggctaaa gtccgccgat ttaccatcgc cgcccagcaa
    58021 caaatgcagc gtgccgtcta cgtgcaggcc attcagcgcc gcttccgtac tgccgacgtt
    58081 ggtcgctttt gaatcgttaa tccaacgtac gccgttatgc tccagcacaa cttcaaagcg
    58141 atgcggcaga ccagtgaatg tggttaacgc tttcaggctg ctggcacgcg gtaaccctgc
    58201 ggcatctgcc agtgccagcg ccgccagcgc attggtgtag ttatgctgcc cggaaagttt
    58261 catctctttc acgttcagca ctttctcgcc tttaacccgc agccaggttt cgccctgctg
    58321 atgattcagg tgatagtcac ccatgttgac gccaaagctg acgcagcgtt catccgcacc
    58381 gcgaatcggc attgttaagg catcatcggc attaaccacg caaacttttg cgttttcgta
    58441 aatgcgcagt tttgctgcac gatactgttg taaaccaaac ggatagcgat ccatatgatc
    58501 ttcagtcacg ttcagaatgg tcgctgctac cgcctgtaag ctagaggtgg tttccagctg
    58561 gaagctcgac agttccagca cgtacagttc gcactcatca tccagcagca tcaacgcagg
    58621 caggccaata ttgccaccca ctccaacgtt aacccccgcc gctttcgcca tttcacccac
    58681 tagcgtggtg accgtgcttt tgccgttaga accggtaatc gccacaatcg gtgcttgtgc
    58741 ttcgcgacaa aacagctcga tatcgccaac gatttcgatt ccggcatcag cggcagcgct
    58801 taaggatgga tgcgccagtg caataccggg actggcgaca atcagatctg ccgccatcag
    58861 ccattcatca ttcagactgc ccgtgtggcg ttctacggct tcgggtaatt tatccaggcc
    58921 aggcggtgtc atacgcgtat ccataacgcg cggcgtcaca ccgcgagcga ggaaaaagtc
    58981 cacgcaggaa agcccggtga ggcccaggcc gataatgaca acatttttac cctggtaatc
    59041 agccatgatt aacgtacctt cagcgttgcc agaccaatca ggaccagcat cagcgaaata
    59101 atccagaaac gcacaatgac gcgcggttcc ggccagcctt tcagttcata gtggtgatga
    59161 atcggtgcca tgcggaaaat acgttgtccg cgcagtttaa aggagccgac ctgcaggatg
    59221 acagaaagcg tttctaccac gaacacgccc cccataatca ccagcaggaa ttcctgacgt
    59281 agcagtacgg cgataatgcc taacgcacca cctaacgcca gcgacccgac atcgcccata
    59341 aagacctgcg ccggataggt gttaaaccac aggaagccca gtcctgcccc gactatcgcg
    59401 gtacagacaa taaccagttc cccggcgtgt cgcagatacg gtatgtgtaa atagctggca
    59461 aagttcatgt tgccggtcgc ccacgccacc agcgcaaaac caccggcgac aaatacggtc
    59521 ggcataattg ccagaccgtc aagaccatcg gtcaggttta ccgcgttgcc agtacccaca
    59581 atgacgaagt aagccagcag aatgtagaac agccccagtt gcggcatcac atctttaaag
    59641 aatgggacca ccagctgcgt tgcgggcgtg tctttgccgg caaggtacag ggcgaaggcg
    59701 acacccagcg caatgaccga catccagaaa tacttccaac gagcgatcaa ccctttggtg
    59761 tctttacgca ccactttgcg ataatcatca acaaagccaa taacaccgta acctaccagc
    59821 accaccaaca cgcaccagac gtacggattg gacgggtaag cccacagcag tacggagatc
    59881 acaatcgccg tcaggatcat aatcccgccc atggtcggcg taccgcgctt gctgaagtgt
    59941 gattcaggac cgtcgttacg caccacctga ccaaaggaaa gtttttgcaa atgagcaatc
    60001 atacgcgggc ccatccacaa tgagatgaac agcgcggtca gcaggctgac gatggcgcga
    60061 aacgtcagat aggaaaagac gttaaagccg gaataatatt tgaccaaatg ttcggccagc
    60121 caaactaaca tgtcccattc tcctgtaaag cgcgtactac ctcttccatg gcggcactac
    60181 gtgaaccctt aactaaaatc gtaattacct gttgctcagc aatcagtgat ttaagacgcg
    60241 taattaacgc agttttatca gcaaaatgtt cgccaacgcc gctggcggtg ctgatagcat
    60301 ggctttgttt acccacgctt aacacgcggt caataccagc agcttttgcc gcctcgccca
    60361 cctgtacatg gcaggcttcg ctttcagcgc ccagttccgc catatcgccc accaccagca
    60421 cgcggtagcc cggcatttca gccagtacct ggactgctgc agtcattgaa ccgacattgg
    60481 cgttgtagga gtcgtcgagc agcaactggt tttctgccag ttggatgggg aacagacggc
    60541 ctggaacagc tttcagattt gccagccccg ctttgatagc atcaagcgtt gcgcccacgg
    60601 acatggagag cgcagcggct gccagcgcat tcgcaatatt gtgacgcccc ggcaacggca
    60661 gcagaacatc gacgctaccg gttggggttt gtagggtaaa ttccgtaccg tgcgaggtca
    60721 catggatatt ggtggcggtg aaatcgctgt tggcggcatt gggtgagaaa cgccacactt
    60781 tgcgtgagcc aattacgctc tgccagttca gccagtcgtt gttgtcggcg ttcataatgg
    60841 cgataccgtt ttccggcagg ccgctaaaga tttcaccttt cgctttcgcg acacccgcaa
    60901 gcgagccaaa accttccaga tgcgccgctg ccaggttgtt gaccagcgca gcttccgggc
    60961 gagtcagact cacagtccag gctatttcgc cctgatggtt cgcgccaagt tcaataactg
    61021 cgtaatcgta ttccggcgtt aagcgcaaca gcgtcatcgg tacaccgatg tcgttgttga
    61081 gattgcctgc cgtataaagc gtgttgccgc actggcttaa aatcgccgcc gtcatctctt
    61141 taacggaggt tttgccggag gaccccgtca gagcaaccac gcgcgccgga acttgctggc
    61201 gaacccatgc agccagttca ccaaacgcca gacgcgtatc cttgacgatt aactgcggca
    61261 ggtcgatgtc cagcggacgg ctaaccagta gtgcgcctgc gccgccagct ttcgcctggt
    61321 cggcaaaatc gtgggcatca aaacgttcgc ctttcagggc aacaaacagg cagcccggcg
    61381 tcagttttcg ggtatcagtg gttacagcat caagggtgat atctgcacct tgcagttcac
    61441 cgttgagaat gtcggtaagt tggctaaggg ttacgctaat catgcaatca cccccagcag
    61501 acgcgccacc gtgacgcgat cggagtagtc cagacgctga ttgccaacaa tctggtaatc
    61561 ttcatggcct ttgcccgcga ccagtaccac atcattctct ttagcctgca taacggcgca
    61621 agtcaccgct tcagcacggc cttccatcac tttggcatgt ccggcatcta acattcccgc
    61681 cagaatatcg ttgatgatgg cacgcggttc ttcggtacgc gggttatcgt ccgtcaccac
    61741 cgccacgtca gcaaactctt cggcaattgc gcccatcagt ggacgcttac ctttatcgcg
    61801 atcgccacca cagccaaaga cgcaccacag cttgcccgca cagtgcaggc gcgccgcctg
    61861 taaggctttt tccagtgcat ccggcgtatg cgcgtaatcc accaccaccg tcggtttgcc
    61921 tggcgcagtg aacacttcca tacgtccgca aaccggttgc agacgcgcgg cggtttttaa
    61981 cagatcagcc agcgggtagc caagtgccag cagtgtcgcc agcgcgagca gcaggttgct
    62041 gacgttaaaa gcgcccatca gatggctttc aatttcgcca tcgccccaac ttgaactaaa
    62101 gcgaatggtc gcgccgctgt cgtgatagtt cacttcggtc gctttcaacc agcgtccgtg
    62161 acagttcgga ttgatatgat cttccattga taccgcaacc gcgtccggca gttttgccag
    62221 ccagcggcgg cccacttcat cgtcggcgtt aacaatcgcc tgaccacaat gatgttcaga
    62281 ataaagcagc catttcgcgg cttcgtagtg ttccatatca ccatgataat caaggtgatc
    62341 gcggcttaag ttggtaaaga ccgacgccgc aaatttcaat gccgccacac ggtgctgtac
    62401 cagcccgtgg gaggaaactt ccattgcgca aaacgtcgcg ccctgatcca ccagccccgc
    62461 cagctcatgc tgaacatcga ctgccgaacc ggtggtattt tctgtcggga tcactttccc
    62521 cagcagaccg ttaccaacgg tgcccattac cgcgctggtt tcgccaagca gttggctcca
    62581 ctgcgccagc agctgggtag tcgtggtttt gccgttggtg cccgttacgc ctacgagacg
    62641 taaattgtca gagggttcat ggtaaaagcg gcccgccagt gcagataaac gctcgttgag
    62701 ctggctgaga tagatgaccg gtacgccgtg catttcacgg atttcaccgt cggtcgcctc
    62761 acctttcgcc tctgcaataa tggcagccac accttgcgct atcgcctgcg ggatatatcg
    62821 acgcccgtcc gcctgatgac ctactacagc tacaaagaga tcgcccgccg cagccacacg
    62881 gctgtcgagt gtcatctctc gcagtgctcg cgaaggtgcg tctggcaccc acggagcaag
    62941 aaggtcgcgc aaattacgat ctgccacctg tcccctcgcc ttgattaatc acaaattcat
    63001 ttttatcgcc cgttgtcagc gcatccggct cgatgttcat ggtacgcaat acgccgccca
    63061 tgatggcacc aaagaccggc gctgaaacgg cgccgccgta gtatttaccc gcctgcggat
    63121 cgttgataac aacaaccagc gcgaagcgcg gctgactcgc aggcgcaacg cctgcggtat
    63181 aagcaatata tttattgatg tagcgaccgt ccggcccgac ctttttcgcg gtaccggttt
    63241 taatggcgat acgatagcct ttaatcgccg ccttcacgcc gccgccgcct ggtagcgcca
    63301 cgctttccat catatgcacc acagtgcgga caatggattc cgggaagaca cgttcaccgg
    63361 gaaccggggg gtcaactttg gtaatcgaca gtgggcgata aatgccgtag ctgccgatag
    63421 ttgcgtagac tcgcgctaac tgtaacggtg ttaccattag cccgtagccg aaagagaagg
    63481 tggccctctc tatgtcagac caccgttgtt tttgaggata taagccactg cgttctccga
    63541 ccaaccccaa attggtcgct tttcccagtc caaaacgtga gtaagtatct actaacgctg
    63601 aggacggcat cgctaacgcc agcttggaaa caccgacgtt actcgacttc tgtaataccc
    63661 cggtcagggt taattcgctg tagcgtgcca cgtctttgat ttcgtggccg ttaattcgat
    63721 aaggaatggt attgagtacc gagttttccc gcaccacgcc acgttgcaac gcggtcatta
    63781 ccaccatcgg tttaaccgtt gagcccggtt caaacacgtc ggtgatggta cggttacgca
    63841 tcgcctcttt cggcgtgccg ctcagattgt tagggttgta tgacgggctg ttagccatcg
    63901 ccagcacttc accggtgttg acatccacca gcacggcgct accagattca gccttgttaa
    63961 aggccaccgc gttgttcagt tcgcgataaa ccagcgcctg caggcgttca tcaatactca
    64021 gcgccaggtt gtgcgctgcc tggctgtcag tagaagaaat atcttcaatt acgcgaccat
    64081 agcggtcttt acgcacaatg cgctcacccg gctgcccggt aagccattta tcgaaactct
    64141 tctcaacgcc ctcaatccct tgactatcga cgttagtaaa gccgatgagg tgagcagtca
    64201 cttcgccgga cggatagtaa cggcgagact cttcacgcag atgaatcccc ggcagtttca
    64261 gttttttgat gtagtccgcc atgtcagggt tcacctgacg cgccagataa ataaagcgcc
    64321 ctttcgggtt ggcgttaatg cgggctgaaa gctgatccag cggaatattg agcgcgttag
    64381 ccagcgcctt ccagcggtca ccgacgctga taccgccagc gtcatgcact tctttcgggt
    64441 cagcccaaat cgcttttacc ggcacgctca ccgctaacgg gcgaccagaa cggtcagtaa
    64501 tcatgccgcg ggaggtggaa acttgctgaa cgcgaagaga acgcatgtcg ccctctttca
    64561 ccagcatatc cggggagata acttgtaacc acgctacgcg tccgagcaga aaagccagcg
    64621 ccaggagaat acagccgcat aacaacgcaa aacgccaact gataaagttg gcatgttctt
    64681 cctgacgttt tggtttctgc gttttcgccg ctgctttcat gcgtcgcgtt tatccttatt
    64741 tttgcactac gatattttct tgtgacggat caacatgctg catttgcagc ttttccgtgg
    64801 cgatcctttc cacccggcta tggtcgccga gcgcattctc ttcaaggatc aggttgcgcc
    64861 attcaatgtc taaagcatct cgctccagca ccagttgttc gcgctgagcg gtcagtaaac
    64921 gggtatggtg cgccgtggtt accacagtca ccgccgtcaa aataatgcaa atgaacaggc
    64981 agagtggcag cttcccaaat cgcaaaagat cgtcaccgat aacaccaggc aatgcatggc
    65041 gctcgtggct tcccatcgat cctttaactt tgcttagagc ttctgtcact ctgctgatca
    65101 tgcattcgtc ctctctgcaa tacgcagaac tgaactacgg gcacgagggt tctcagccac
    65161 ctcttcttcg cccggcatta acttgcctag tgctcgcagc tgacggccac ccagtttttt
    65221 gagctgctct tcagtcatcg gtaaccctgc cggaacttgc ggaccgcggc tgttttcacg
    65281 cataaaacgt ttcacaatac ggtcttccag cgagtggaag ctgatgatcg aaagccgccc
    65341 acccggggcc agcacgttga gcgagctttt tagcgcctgc tctatctcct ccagttcact
    65401 gtttacccaa atgcgcaccg cctggaaggt acgggtcgcg ggatgtttaa acttatcttt
    65461 caccggcgtt gcagcagcca cgacttccgc cagttctttg gtgcgggtca tcggctgttc
    65521 gcggttacgc tcgacaatgg cgcgggcaat gcgtttggca aaacgctctt caccataggt
    65581 tttcaatacc caggcgatat cggcttcttc tgcggtttgt agccattcag cggctgactg
    65641 cccacgggtt gggtccatac gcatgtccag cggaccatcg cgcataaagg aaaagccgcg
    65701 ctcagcatca tcaagttgcg gtgaagagac gccaagatcg aggagaatgc cgtcgatctt
    65761 gccgataaga tcgcgctcgg caacgtattc gcccagcgcg gagaaaggtc cgtggatgat
    65821 ggagaagcgc ggatcatcaa tagtcttcgc aacggcgata gcctgcgggt cgcgatcgat
    65881 cgccagcaaa cgcccctctt cgccaagctg cgagaggatc agacgtgagt gaccaccgcg
    65941 accaaaagtc ccatcaatgt agatgccatc aggacggata ttgaggccat taacggcttc
    66001 atccagcagc accgtagtat gtttatagtt ttccatcatt ttatagagac aagtcctgca
    66061 gtcgctccga taagtctccg gtagccaact gctctgcgtc gatatcttcc ttgacctgtt
    66121 gatgccaggt tgtttcatcc cacagctcaa acttgttgaa ctgtccaacc agcatcactt
    66181 cttttgtcag cccggcatgt tgccgcagta ctggcgcgat taacaatcga cctgcgccat
    66241 ccatctgaca ttcgctggca tgacctaaca gtaggcgctg cacacggcgc tcaaccgggt
    66301 tcatgctcga cagacgcgat aatttttgct cgataatttc ccattcaggc agggggtaaa
    66361 gcagcaggca cgggtgatga atgtcaatgg tgcaaaccat ttgaccggca gcgttctcaa
    66421 gcagctgttc ccggtaacgg gtaggcactg ataagcgccc tttgctgtcg agattgacta
    66481 acgttgctcc ccggaacatg ccagcctcac cccttattcc cactttgccc cacaatttcc
    66541 cactgaaagg agtttacgga gcggaggaaa agcttgtcaa gccagtcaca aggcataccg
    66601 aggtaaaaaa cccgatgttt acggctaata tcagcgttgt ttattttacg tccaacgtgc
    66661 gcagcaaatt aacgttatga atagctgtaa gaaaaaatcc ctcatcgtca tcaccgtttt
    66721 aaaatgactc actattttcc acagcaaata taaagtgtca gtttgcgacg cgagcggcat
    66781 tttaggacat atcttccccg gttaacagtc cctgttgcgt ctgtgtggcg cttgatgcca
    66841 gcgggtttgc gcaaaagcaa agaaaagtgc ttgttaaatc ggcaattcat ctctgcatgt
    66901 aacaaaataa tgcaaaaacg cgctgtcgat atcacttaat tattgtgaat ttcagctggt
    66961 gagttaatta acgggcgggt ttaaaagaac ttatcaataa tagtcaggaa tagtcttatt
    67021 tactttaagg atattgatgt ccattcccgt actctacgcg ccagagggaa attcacctgg
    67081 cgcgtatttt tgttcgcggc ttagctacgg ctgagcacac cgcggcgata gagattacgt
    67141 ttaatgcgcg ttaaaccagg ttttggctta cgcggttcgt ccaggcttgc caggacaatc
    67201 tccagcacac gctctgcgac atcgcggtga cgttgagcca ctgccagcac cgggcactgt
    67261 aagaagtcga gcagttcgtt atcgccaaag gtggcaattg ccaggtcaga aggcagttta
    67321 ccatcgcgac gtagcgtgac atccatcacc ccttgcaaca acgcaaacga cgttgtgaac
    67381 agcgcctgcg gcatcggatg cgtttccagc catttttcga ataactgggc agccgcctcc
    67441 cgctcatagc tgttggcata caggaaatgc acttcgcgcg gatcatcttt ccaggcagta
    67501 cggaaacctt gttcacgcag gaagctgaca gaaagctccg gcagcgcgcc aagataaagc
    67561 accgtctcgg cgggaaactt acgtaactct tccgccagca tttcggcatc atcctgatcg
    67621 gcaccaacca cgctggtgaa atgttcacga tcgagggcgc ggtccagcgc gacaatcggg
    67681 aacgggtcgt tagcccagcg ttgataaaaa ggatgctcag gtggcaacga cgtcgaaaca
    67741 ataatggcat caacctgacg ctgtaaaagg tgctcaatgc agcgcatttc gttgtctggc
    67801 tgatcttctg agcaggcaat cagcagttga taaccccgtt gccgcgcctg gcgctcaaga
    67861 tagttagcga tgcgggtata gctggtgttc tccagatcgg ggatcacaag accaatagaa
    67921 cgtgtgcgtc cagcacgaag cccagctgcc acggcgttcg ggtggtaatt gtgctcacgc
    67981 accacagcca tgactttttc aacggttttg tcgctcacac ggtattgctt cgctttgccg
    68041 ttaataacat agcttgcagt ggtccgcgac actcccgcca gccgagcgat ttcatccagt
    68101 ttcacaattg ccccttgcgt aaaatgtaaa aaccatagcc attgtactgg tatgggttaa
    68161 attcattaac atctaagcgc agaaaattaa ctgcggcaac tgcttttatt ccaggttccc
    68221 actgatttcg caaaaaaagc ccaacgtgac atgttgggct gcaaattgcg cactgagatc
    68281 attctcaacg cattatttta tcgccgcgcg aaagtccgac cacaccagag cgagcaactt
    68341 ccacaatttt cgccacatcg cgaatcgatg ctaaaaatgc atcaagctta tcgctggtgc
    68401 ctgctaattg aacggtataa agcgaggggg tgacatcgat aatttgccca cggaatattt
    68461 ccgtattacg tttcacttcg tcacgcccgt aaccgctggc ctgaattttc accagcatga
    68521 tttcccgctc aacatgcgcg ccctgcccca actcactcac gcgcaagaca tcgaccagct
    68581 tgtgtaattg cttttcgatc tgctcaagca ctttttcatc gcccacggtc tggatggtca
    68641 tacgcgataa tgtcggatcg tcggttggcg caacggtcag gctttcaatg ttgtagccac
    68701 gctgggaaaa aaggccaatc acgcgggata acgcgcctga ttcattttcg agtaagactg
    68761 ataatatccg gcgcataatc aggttctctc cgttttgctt aaccacattt catccattcc
    68821 gcccccgcga atctgcatcg ggtagacgtg ctcactgcca tcgacggtaa catcaacaaa
    68881 caccaggcga ttattgcaca cctgttccag cgcctcgcta agtttgcttt ccagctcatg
    68941 cggatgagag atctggatcc cgacgtgacc ataggcttcc gccagacgga cgaaatcggg
    69001 tagtgattgc atataagatt gtgaatgacg gccggaatag atcatgtcct gccactgttt
    69061 caccatcccc agatagcggt tattgagatt caccaccagt accggcaact cgtattgcaa
    69121 cgcggtagac agttcctgga tgttcatctg aatactgccg tcgccagtga cgcaaaccac
    69181 ggtttcttct ggcaacgcca ttttgacgcc cagtgccgca ggtaaaccaa aacccatcgt
    69241 gccgaggcca ccggaattga tccagcgacg cggtttgtcg aatggataat aaagtgcagc
    69301 aaacatctgg tgctgcccga catcggacgt cacgtaagcg tctcccttcg tcaaccgcca
    69361 aagagtctcg atcaccgcct gcggtttaat cttttcactg tgagtgtcat atttcaggca
    69421 ctgacgagcg cgccactgtt caatttgctg ccaccagtcg cggatctcat ccagtggttg
    69481 atgggcggat tcttgcgaca agagttcaag catttgttcg aggacctggc gagcatcccc
    69541 cacaatcggg atatccgcag tcacggtttt agaaatggaa gtaggatcaa tatcgatatg
    69601 cagaacagtg gcatttgggc agtactttgc cagattgttc gtcgttcggt catcaaatcg
    69661 taccccgacg gcgaaaatca catccgcgtt atgcatcgtc atattggctt cgtaggtacc
    69721 gtgcattccc agcatgccca gtgcctgacg atgcgttgcc ggaaacgccc ccagccccat
    69781 caatgaggaa acaacgggca gattcaacgc ctccaccgtt tctttcaact gctgatggca
    69841 gcccgccatg attgccccac cgcctacgta gacaaccggt ttttttgccg ctaccagcgt
    69901 ttgcagagcg cgcttaattt gccctttatg tccggtagta gtgggattgt aagaacgcat
    69961 actgaccgac tcaggccaga catagggtaa tttgttcgcc ggattaagaa tatctttcgg
    70021 taaatcaacg accactggtc ctgggcgacc acttgccgcc agccagaaag cctttttcag
    70081 cacctgcgga atgtcttccg tttgcttaac cagaaaactg tgtttaacca ccggtcgcga
    70141 aatccccacc atgtcgcact cctgaaaggc atcgtaacct atcaacgagg tcgctacctg
    70201 cccggaaagg acaactaatg gaatggaatc catataagcg gtggcgatgc cggtaatcgc
    70261 attggtcgcc cctggacccg acgttaccag cacgacgccg acttccccgg tcgcgcgcgc
    70321 cagaccatcg gccatatgca ctgccgcctg ctcatgacga actaaaacat gatcgatacc
    70381 acccacggta tgcaatgcat cgtaaatatc aaggactgcg cctccgggat aaccgaatac
    70441 ttgtttaacg ccctgatcga taagcgatcg gacgaccatc tcggctccag acaacatctc
    70501 catggcctgc ctcactgttt gacggaaaaa atgtgtaaaa cacattaacc gtccgatctt
    70561 gcttaagcaa ttggcagcca gagacataaa cgcagggtaa taagaataaa caggaggaaa
    70621 ggtgaaaaga aacgataaaa tcctccatta cgaaggatta aattgataat aaatcggctg
    70681 aatcccacaa cttacagcaa tttctctatt tttttaatta atcctaaaat agagagccca
    70741 cacattcaga ataaaattct acattcaata aaaaatacag aataaaccag acattcatgt
    70801 ctgacctatt ctgcattcag ttagcgtttg caaattgaga ctaattgctc ttccatccac
    70861 tgatggcctt tatcgcgccc ggcagcttca tgccaggaga gataacaggt tctgctattt
    70921 tgttttaacg gcagcggtaa tacctgtaat tctaaggatt cagcgaactc ttcagccagc
    70981 caacgcggcg caatagcgac caaatgcgtt tgcgacacca cgctaagtac gctcatcatt
    71041 gccatgccct gatacgcgat actggcttgc ttatctaccg tgtcatacca aggttgacta
    71101 aatgacgcga aacgatcgag cgaaaccgcc gcatgttgtt cgttataaac atcatgtttc
    71161 agtaacgggc ccttaattgt tggatgattt ttgctggcta ccagcaccat ttcatcttta
    71221 aataatggca cgctggtaaa ttcaggacga tggaagtctt cataactaat cacaaactcc
    71281 gtttcctgat aacgcagctg atgttcagtg ttctgattta atgaagactt gaacataaca
    71341 tgtatatttg gcgcaatctg ctcaatgtga ttataaatct gcgaggtcag aatgctgtct
    71401 aacgggctgc aaacacaaag atgaaataca cgttcactgc tcgcgggttc aaaacctgaa
    71461 ccaggcaatt cattttgtac tagttgcaat gcctgacgaa ctgagccaaa aagttgaaat
    71521 gcgcgagcag tcggttgaat accacggcca taacgaacaa aaagctcgtc attaaacatc
    71581 accttcaggc gtgcaacagc gttactgacc gcaggttgcg acatacccag aacatgagcg
    71641 gcacgggtaa tgttttgctc ctgcatcacg gcatcgaaaa cggttaataa gttgagatcg
    71701 accatgcgta gctgtggctt gcttaactcc gccgtctctg aatgatctgt ttgtacctct
    71761 ggcatactta actccactgt cacacttaac tccctttccc ttattggaat gcagagaaat
    71821 aatcctgaag aatatgattt atcatgcata taaaataaga aaaagcaaaa tgagtaaaat
    71881 tcggaaaaca taaagatacg ggttctgata aaaacagaat ccattgccgt ggaatgagtc
    71941 atttaccaca gcataataat ccataatgtt tttgttgcga aaacaatcta atcataacta
    72001 ccgcga
//
LOCUS       DAJOWB010000010        70039 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJOWB010000010 DAJOWB010000000
VERSION     DAJOWB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 70039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.010"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(95..3244)
                     /locus_tag="OHO85_000753"
     CDS             complement(95..3244)
                     /locus_tag="OHO85_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCQ2670176.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3267..4460)
                     /gene="acrA"
                     /locus_tag="OHO85_000754"
     CDS             complement(3267..4460)
                     /gene="acrA"
                     /locus_tag="OHO85_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCQ2670177.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINQAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4602..5249
                     /gene="acrR"
                     /locus_tag="OHO85_000755"
     CDS             4602..5249
                     /gene="acrR"
                     /locus_tag="OHO85_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCQ2670178.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5377..8739
                     /gene="mscK"
                     /locus_tag="OHO85_000756"
     CDS             5377..8739
                     /gene="mscK"
                     /locus_tag="OHO85_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCQ2670179.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(8951..9112)
                     /gene="rsmS"
                     /locus_tag="OHO85_000757"
     CDS             complement(8951..9112)
                     /gene="rsmS"
                     /locus_tag="OHO85_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCQ2670180.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9126..9653)
                     /gene="priC"
                     /locus_tag="OHO85_000758"
     CDS             complement(9126..9653)
                     /gene="priC"
                     /locus_tag="OHO85_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCQ2670181.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9723..10100
                     /locus_tag="OHO85_000759"
     CDS             9723..10100
                     /locus_tag="OHO85_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCQ2670182.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10253..10804
                     /gene="apt"
                     /locus_tag="OHO85_000760"
     CDS             10253..10804
                     /gene="apt"
                     /locus_tag="OHO85_000760"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCQ2670183.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10933..12864
                     /gene="dnaX"
                     /locus_tag="OHO85_000761"
     CDS             10933..12864
                     /gene="dnaX"
                     /locus_tag="OHO85_000761"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCQ2670184.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12197..12261
                     /gene="dnaX"
                     /locus_tag="OHO85_000761"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12917..13246
                     /locus_tag="OHO85_000762"
     CDS             12917..13246
                     /locus_tag="OHO85_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCQ2670185.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13246..13851
                     /gene="recR"
                     /locus_tag="OHO85_000763"
     CDS             13246..13851
                     /gene="recR"
                     /locus_tag="OHO85_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCQ2670186.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            13961..15835
                     /gene="htpG"
                     /locus_tag="OHO85_000764"
     CDS             13961..15835
                     /gene="htpG"
                     /locus_tag="OHO85_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCQ2670187.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16016..16660
                     /gene="adk"
                     /locus_tag="OHO85_000765"
     CDS             16016..16660
                     /gene="adk"
                     /locus_tag="OHO85_000765"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCQ2670188.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16896..17858
                     /gene="hemH"
                     /locus_tag="OHO85_000766"
     CDS             16896..17858
                     /gene="hemH"
                     /locus_tag="OHO85_000766"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCQ2670189.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDDLLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17855..18814)
                     /gene="aes"
                     /locus_tag="OHO85_000767"
     CDS             complement(17855..18814)
                     /gene="aes"
                     /locus_tag="OHO85_000767"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCQ2670190.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQSDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            18966..20270
                     /gene="gsk"
                     /locus_tag="OHO85_000768"
     CDS             18966..20270
                     /gene="gsk"
                     /locus_tag="OHO85_000768"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCQ2670191.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20403..22079)
                     /locus_tag="OHO85_000769"
     CDS             complement(20403..22079)
                     /locus_tag="OHO85_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCQ2670192.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22317..23537)
                     /gene="fsr"
                     /locus_tag="OHO85_000770"
     CDS             complement(22317..23537)
                     /gene="fsr"
                     /locus_tag="OHO85_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCQ2670193.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGWHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23755..25407
                     /gene="ushA"
                     /locus_tag="OHO85_000771"
     CDS             23755..25407
                     /gene="ushA"
                     /locus_tag="OHO85_000771"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCQ2670194.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25444..25923)
                     /gene="ybaK"
                     /locus_tag="OHO85_000772"
     CDS             complement(25444..25923)
                     /gene="ybaK"
                     /locus_tag="OHO85_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCQ2670195.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26127..26921)
                     /locus_tag="OHO85_000773"
     CDS             complement(26127..26921)
                     /locus_tag="OHO85_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCQ2670196.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            27059..27400
                     /locus_tag="OHO85_000774"
     CDS             27059..27400
                     /locus_tag="OHO85_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCQ2670197.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27615..30119)
                     /gene="copA"
                     /locus_tag="OHO85_000775"
     CDS             complement(27615..30119)
                     /gene="copA"
                     /locus_tag="OHO85_000775"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCQ2670198.1"
                     /translation="MSQIIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30381..31313
                     /gene="glsA"
                     /locus_tag="OHO85_000776"
     CDS             30381..31313
                     /gene="glsA"
                     /locus_tag="OHO85_000776"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCQ2670199.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGKVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31316..32608
                     /locus_tag="OHO85_000777"
     CDS             31316..32608
                     /locus_tag="OHO85_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCQ2670200.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            32733..33140
                     /gene="cueR"
                     /locus_tag="OHO85_000778"
     CDS             32733..33140
                     /gene="cueR"
                     /locus_tag="OHO85_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCQ2670201.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(33141..33599)
                     /locus_tag="OHO85_000779"
     CDS             complement(33141..33599)
                     /locus_tag="OHO85_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCQ2670202.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(33596..34513)
                     /locus_tag="OHO85_000780"
     CDS             complement(33596..34513)
                     /locus_tag="OHO85_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCQ2670203.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            34659..35336
                     /gene="fetA"
                     /locus_tag="OHO85_000781"
     CDS             34659..35336
                     /gene="fetA"
                     /locus_tag="OHO85_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCQ2670204.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKDIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            35323..36102
                     /gene="fetB"
                     /locus_tag="OHO85_000782"
     CDS             35323..36102
                     /gene="fetB"
                     /locus_tag="OHO85_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCQ2670205.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFVAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(36165..37019)
                     /gene="cnoX"
                     /locus_tag="OHO85_000783"
     CDS             complement(36165..37019)
                     /gene="cnoX"
                     /locus_tag="OHO85_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCQ2670206.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(37080..37889)
                     /gene="ybbO"
                     /locus_tag="OHO85_000784"
     CDS             complement(37080..37889)
                     /gene="ybbO"
                     /locus_tag="OHO85_000784"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCQ2670207.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(37879..38502)
                     /gene="tesA"
                     /locus_tag="OHO85_000785"
     CDS             complement(37879..38502)
                     /gene="tesA"
                     /locus_tag="OHO85_000785"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCQ2670208.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            38473..39159
                     /locus_tag="OHO85_000786"
     CDS             38473..39159
                     /locus_tag="OHO85_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2670209.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            39156..41570
                     /locus_tag="OHO85_000787"
     CDS             39156..41570
                     /locus_tag="OHO85_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2670210.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(42282..43376)
                     /gene="mnmH"
                     /locus_tag="OHO85_000788"
     CDS             complement(42282..43376)
                     /gene="mnmH"
                     /locus_tag="OHO85_000788"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCQ2670211.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEHGAMPAAINLPLMN
                     NDERAAVGTCYKQQDSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEVGINYPLVEGGYKVLRQTAIQATIELSQKPIVLIGGCTGCGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAQ"
     gene            complement(43445..44371)
                     /gene="allS"
                     /locus_tag="OHO85_000789"
     CDS             complement(43445..44371)
                     /gene="allS"
                     /locus_tag="OHO85_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCQ2670212.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            44601..45083
                     /gene="allA"
                     /locus_tag="OHO85_000790"
     CDS             44601..45083
                     /gene="allA"
                     /locus_tag="OHO85_000790"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCQ2670213.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            45161..45976
                     /gene="allR"
                     /locus_tag="OHO85_000791"
     CDS             45161..45976
                     /gene="allR"
                     /locus_tag="OHO85_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCQ2670214.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            46066..47847
                     /gene="gcl"
                     /locus_tag="OHO85_000792"
     CDS             46066..47847
                     /gene="gcl"
                     /locus_tag="OHO85_000792"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCQ2670215.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            47860..48636
                     /gene="hyi"
                     /locus_tag="OHO85_000793"
     CDS             47860..48636
                     /gene="hyi"
                     /locus_tag="OHO85_000793"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCQ2670216.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            48736..49614
                     /gene="glxR"
                     /locus_tag="OHO85_000794"
     CDS             48736..49614
                     /gene="glxR"
                     /locus_tag="OHO85_000794"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCQ2670217.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            49783..51237
                     /locus_tag="OHO85_000795"
     CDS             49783..51237
                     /locus_tag="OHO85_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HCQ2670218.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPAFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGKINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            51297..52658
                     /gene="allB"
                     /locus_tag="OHO85_000796"
     CDS             51297..52658
                     /gene="allB"
                     /locus_tag="OHO85_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HCQ2670219.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            52715..54016
                     /locus_tag="OHO85_000797"
     CDS             52715..54016
                     /locus_tag="OHO85_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HCQ2670220.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            54038..55183
                     /gene="glxK"
                     /locus_tag="OHO85_000798"
     CDS             54038..55183
                     /gene="glxK"
                     /locus_tag="OHO85_000798"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HCQ2670221.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANSHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(55411..56196)
                     /gene="allE"
                     /locus_tag="OHO85_000799"
     CDS             complement(55411..56196)
                     /gene="allE"
                     /locus_tag="OHO85_000799"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCQ2670222.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(56207..57442)
                     /gene="allC"
                     /locus_tag="OHO85_000800"
     CDS             complement(56207..57442)
                     /gene="allC"
                     /locus_tag="OHO85_000800"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCQ2670223.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(57464..58513)
                     /gene="allD"
                     /locus_tag="OHO85_000801"
     CDS             complement(57464..58513)
                     /gene="allD"
                     /locus_tag="OHO85_000801"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HCQ2670224.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGNEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHVVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            58830..60497
                     /locus_tag="OHO85_000802"
     CDS             58830..60497
                     /locus_tag="OHO85_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCQ2670225.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGAGSSQAL
                     TQVRRWDSACQKLPDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            60507..61766
                     /locus_tag="OHO85_000803"
     CDS             60507..61766
                     /locus_tag="OHO85_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCQ2670226.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWVKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIWVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            61777..62592
                     /locus_tag="OHO85_000804"
     CDS             61777..62592
                     /locus_tag="OHO85_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCQ2670227.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWMLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            62589..63482
                     /locus_tag="OHO85_000805"
     CDS             62589..63482
                     /locus_tag="OHO85_000805"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCQ2670228.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(63621..64688)
                     /gene="purK"
                     /locus_tag="OHO85_000806"
     CDS             complement(63621..64688)
                     /gene="purK"
                     /locus_tag="OHO85_000806"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCQ2670229.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLSATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(64685..65194)
                     /gene="purE"
                     /locus_tag="OHO85_000807"
     CDS             complement(64685..65194)
                     /gene="purE"
                     /locus_tag="OHO85_000807"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCQ2670230.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(65312..66034)
                     /gene="lpxH"
                     /locus_tag="OHO85_000808"
     CDS             complement(65312..66034)
                     /gene="lpxH"
                     /locus_tag="OHO85_000808"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCQ2670231.1"
                     /translation="MATLFIADLHLCVEDPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQGLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(66037..66531)
                     /gene="ppiB"
                     /locus_tag="OHO85_000809"
     CDS             complement(66037..66531)
                     /gene="ppiB"
                     /locus_tag="OHO85_000809"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCQ2670232.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            66705..68090
                     /gene="cysS"
                     /locus_tag="OHO85_000810"
     CDS             66705..68090
                     /gene="cysS"
                     /locus_tag="OHO85_000810"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCQ2670233.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(68126..68647)
                     /locus_tag="OHO85_000811"
     CDS             complement(68126..68647)
                     /locus_tag="OHO85_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCQ2670234.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FNFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(68755..68967)
                     /gene="ybcJ"
                     /locus_tag="OHO85_000812"
     CDS             complement(68755..68967)
                     /gene="ybcJ"
                     /locus_tag="OHO85_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCQ2670235.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(68969..69835)
                     /gene="folD"
                     /locus_tag="OHO85_000813"
     CDS             complement(68969..69835)
                     /gene="folD"
                     /locus_tag="OHO85_000813"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCQ2670236.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
BASE COUNT        16517 a        18082 c        18798 g        16642 t
ORIGIN      
        1 tgagatcctg agttggtggt tcaattactc cttaatgttc gtaggttatg cataaaaaag
       61 gccgcttgcg cggccttagt gattacacgt tgtatcaatg atgatcgaca gtatggctgt
      121 gctcgatatc ttcattcttg cggctaaagc ggcggcgaac cacaacaaag aataccggaa
      181 cgaagaagat tgccagtacc gttgcggtca ccatcccgcc cattacaccg gtacctactg
      241 cgttctgcgc gccggaacca gcaccagtac tgataaccag cggcataacg ccgaggataa
      301 acgccagcga ggtcatcagg atcggacgta aacgcatccg cactgcatca agcgtcgctt
      361 caatcagacc tttaccttct ttatccatca agtctttggc gaattcgacg ataagtatcg
      421 cgttcttcgc cgacaaccca atggttgtga gcaggcctac ctggaagtaa acgtcattgg
      481 tcaggccacg gaaggtggca gccagcaacg caccgataac ccccagcgga acgaccagca
      541 taacggagaa cggaatcgac cagctctcgt acagcgccgc cagacacagg aatacgacaa
      601 tcaacgaaat cgcgtacagt gaaggtgcct ggttgccgga gaggcgttcc tgataggaca
      661 tccccgtcca gtcatagcca acaccggtag gcagtttgct cgccagttgt tccattagct
      721 ccattgcttc accggtactt ttacccggtg ccgcctggcc taagatttcc atggatggca
      781 ggccattgta acgttccaga cgcggcgaac cgtactccca acgagaagag gagaatgccg
      841 agaacggcac catctgacca tcagcagcac gaacatacca gtcgccgata tcatccggca
      901 gcatacggta tttcgcttct gacatgacgt aaactttctt cacacgaccg cggtcgataa
      961 agtcgttcac atagctaccg ccccatgcag cgcccagagt ggtgttaatg tcgttgatag
     1021 aaacacccag cgcctgcgct ttttcctggt cgatatcaat cttaaactgc ggggtatctt
     1081 ccagaccgtt tggacgtacg ctggtcagca tatcagggtg cttcgctgct tctgcaagca
     1141 gctggttacg cgcctgagtc agtttttcgt gaccaaggcc agcctggtca atcagctcaa
     1201 agtcaaagcc ggttgcagta cccagttcca cgattgcggg caggttaaag gcgaaaacca
     1261 tcgcatcttt gatttgcgag aaagcgcgtg ttgcacgcat ggtaatcgct tcaactttgt
     1321 tttcttcgcc cggacgatcg gcccagtcct tcaaggaaac gaacgcaata ccggtattct
     1381 gaccacgtcc cgcaaagccg aagccgttaa cggcgaacac cgactcaacg ttgttctttt
     1441 ctttggtcag atagtaatgc gttacctcat tgagcacttt ctgcgtacgt tcctgcgttg
     1501 cacctgctgg cagctgaacc atggtcataa atacgccctg gtcctcatct ggcaagaagg
     1561 agcttggcag acgcacgaac agataggcca tgccgaccac gatgatcaga tacagcacca
     1621 ggtaacgccc cgtactgcgc agaataccgc ctacgctgtc ggtgtagtgg tgcgtgctct
     1681 tctcgaacat gcggttaaac cagccgaaga agcctttttt accttccccg tgatcgcctt
     1741 tggcaatcgg tttcagcatg gtggcacaaa gagccggagt caggatcaac gctaccagta
     1801 ccgacagcgc cattgctgaa acaatggtaa tagagaactg acgatagata gcaccagtag
     1861 aaccgccaaa gaaggccatc ggtacgaata ccgccgacag taccatcgcg ataccgacca
     1921 gagcgccctg aatctgcccc atcgacttac gggtagcttc ttttggcggc aaaccttctt
     1981 ccgccataac acgctccacg ttttctacca caacgatagc gtcatccacc aacaagccga
     2041 tagcgagcac catcccgaac attgttagcg tgtttatcga gaagccaaag gcggcaagga
     2101 cggcaaaggt cccgagcaat accaccggta cggcaatggt cggaatcaac gtcgcgcgga
     2161 agttctgcag gaacagatac ataaccagga acacgaggat gatcgcttcg accagcgttt
     2221 taaccacttc gtgaatagag attttcacga acggcgtggt gtcgtacggg taaacaattt
     2281 tcagacccga cgggaagaac ggttccatct tcgccagttc agcacggatt gccgcagcgg
     2341 tatccagcgc gtttgcaccg gtcgccagct tgatccccag accggaagcc ggttggccgt
     2401 taaactctgc gatgatgtcg tagttctcac cacccagctc aatcttcgcc acatcacgca
     2461 gcagtacgcg ggaaccatct tgattcactt tcagcaggat tttgccgaac tcttcagtag
     2521 aggtcagacg cgtctgagca ataatagagg cgttaagctg ttggcctttc accggcggcg
     2581 taccaccgag ctgacccgcc gcaacctggg cgttctgcgc tttgatggcg gtaatgacat
     2641 caaccggcgt tagctggaat ttgttcagct cattcgggtt catccagata cgcatcgcgt
     2701 actgtgaacc gaacaactga acatcaccca cgcccgacgt acggctgatg gcatctttca
     2761 tattcgccgc tacgtagtca gagatatcct cctgcgtcat ggtgccatcg gtgttgataa
     2821 cgccgacaac catcaggaag ctgctggatg atttctcaac gctcacccct tgctgctgaa
     2881 cttcttgcgg cagcaacggc atcgccagct gcagtttgtt ctgcacctga acctgcgcga
     2941 tgtccgcatc agtaccagac tcaaaggtca gggtgatctg cacggtaccc gtggagtcac
     3001 tgttagagga catgtacatc aggttatcga taccgttcat attctgttcg ataacctgtg
     3061 tcaccgtgtc ctgcactgtt ttcgcatcag cgccggggta ggaggcggag atcgttactg
     3121 ccggcggtgc aatcgtagga tattgcgcca ccggcagttt gaggatcgcc agcccccctg
     3181 ccaacatgat gataatggcg atcacccacg caaaaatcgg gcgatcgata aagaaattag
     3241 gcatgtctta acggctcctg tttaagttaa gacttggact gttcaggctg agcaccgctt
     3301 gcggcttgct ggttattatc agcggtaact tcttgtgctt ttacctggac accaggacgc
     3361 actttctgca gcccacttat tactacgcga tcgcctgctt tcagaccttc tgtcaccagc
     3421 cacttatcgc caatagcctg gcttgcaacg atcggacggg tttccacttt gtcatccgcg
     3481 ccaactacca gtacggtggc atcaccacgc ggcgtacggg ttacgccctg ttgcgggact
     3541 aaaatagcgt ttggattaag cccttcttcc agacgtgcgc gcacgaacat acccggcagc
     3601 agagtgtgat ccgggttcgg gaagatagcg cgtagggtga tagacccagt ggtctgatca
     3661 acggtaacgt cagagaattc cagcgtaccg tcctgcggga acttaatgcc gtcactggtg
     3721 atcagcgaca ctttggcttt gccgttctct tgtttcagcg tgccattcgc cagttcctgt
     3781 ttcaggcgca ggaagtcgtt gctggactgg gtcacatcaa cgtagatcgg atcaagttgc
     3841 tgcacggttg ccagcgcagt cgcctgaccg ttctgtacca atgcgccttc cgtcacgttc
     3901 gacttaccaa tgcgaccgct aatcggagag gtgactttgg tgtaagcctg attgatccgc
     3961 gcagtttcaa cggcagcttt cgccgcagtt accgcagcat tcgcctgttg cgcatcagcc
     4021 agagcctgat cgtactcttg cttactgatg tactgagtac cgagcagttt ctgataacga
     4081 ttcaccgtca attgcgcgat attggctgca gcctgggctt tcgccagatc acctttcgca
     4141 ctgtcgtatg tcgcctgata ggtcgcagga tcaatctgat agagagagac accagcttcg
     4201 atgtcgctac cttctttgaa attacgcttc aggataatcc cgctaacttg aggacgaact
     4261 tctgcgatcc ggtaggcact ggtgcgaccc ggaagctcgg ttgtgatctg cagaggttca
     4321 gttttgactg ttactacgcc aacggcgggc atctgctggc caccttgttg ggcctgtttg
     4381 tcgtcacatc ctgttagggc taagctgcct gagagcatca gaacgaccgc cagaggcgta
     4441 aaccctctgt ttttgttcat atgtaaacct cgagtgtccg atttcaaatt ggtcaatggt
     4501 caaaagttaa taaacccatt gctgcgttta tattatcgtc gtgctatggt acatacattc
     4561 acaaatgtat gtaaatctaa cgcctgtaaa ttcacgaaca tatggcacga aaaaccaaac
     4621 aagaagcgca agaaacgcgc caacacatcc tcgatgtggc tctacgtctt ttctcacagc
     4681 agggggtatc atccacctcg ctgggcgaga ttgcaaaagc agctggcgtt acgcgcggtg
     4741 caatctactg gcattttaaa gacaagtcgg atttgttcag tgagatctgg gaactgtcag
     4801 aatccaatat tggtgaacta gagcttgagt atcaggcaaa attccctggc gatccactct
     4861 cagtattaag agagatatta attcatgttc ttgaatccac ggtgacagaa gaacgacgtc
     4921 gattattgat ggagattata ttccacaaat gcgaatttgt cggagaaatg gctgttgtgc
     4981 aacaggcaca acgtaatctc tgtctggaaa gttatgaccg tatagaacaa acgttaaaac
     5041 attgtattga agcgaaaatg ttgcctgcgg atttaatgac gcgtcgggca gcaattatta
     5101 tgcgcggcta tatttccggc ctgatggaaa actggctctt tgccccgcaa tcttttgatc
     5161 ttaaaaaaga agcccgcgat tacgtcgcca tcttactgga gatgtatctc ctgtgcccca
     5221 cgcttcgtaa tcctgccact aacgaataac cctgaatctg actccaggat ttttcctgga
     5281 cattttcgtc gttgctattc tggttcactg cgtcgtgata ttcttgcggt ttgacttttt
     5341 caggtcgttc ttcaggttca gaaaccttca ttcatcatga ctatgttcca gtattacaaa
     5401 cgatcacggc attttgtttt ttcagcattt attgcttttg tttttgtctt gttatgccag
     5461 aacacggcgt ttgcgcgggc gtcatcgaat ggtgatctgc cgacaaaagc ggacctgcag
     5521 gcgcaacttg actcactaaa taaacaaaaa gatctttctg ctcaggacaa actggtgcag
     5581 caggatctga cagatacatt agccaccctc gataaaatcg atcgcataaa agaagagaca
     5641 gttcagctac ggcaaaaagt cgctgaagcg ccggaaaaaa tgcgccaggc gaccgcggcg
     5701 ttaacagcac ttagcgatgt cgataacgac gaagaaacgc gcaaaattct gagtacgctt
     5761 tcgttgcgcc agctggaaac tcgcgttgcc caggcgctgg acgatttgca aaacgcacaa
     5821 aacgatctgg cgtcttataa cagccagctg gtttcgttac agacgcagcc cgaacgcgtg
     5881 caaaatgcga tgtataacgc ttcgcagcag ctgcaacaaa ttcgcagtcg tctggatggg
     5941 actgatgtcg gcgagactgc cttacgtccc agccagaaag tgttaatgca ggctcagcag
     6001 gcgttgctga atgcggagat tgaccagcag cgtaaaagcc tggaagggaa caccgtcttg
     6061 caggatacct tgcaaaagca acgtgattac gtgacggcga acagcgcccg tctggagcac
     6121 cagttgcagc tgttgcaaga agcggtaaac agcaagcgcc tgactttaac tgaaaaaacg
     6181 gcgcaggaag ccgtctcccc ggatgaagcc gcgcgtattc aggctaatcc gctggtgaaa
     6241 caggaactgg aaattaacca gcagttaagt cagcgtctga ttaccgcgac tgaaaacggt
     6301 aatcagttga tgcagcaaaa cattaaagtc aaaaactggc tggagcgggc gctgcaatcg
     6361 gaacgcaata ttaaagagca gattgccgtc ctgaagggca gcctgctgtt gtctcgtatc
     6421 ctttaccagc aacaacaaac gctgccctcg gcggatgaac tggaaaacat gaccaaccgc
     6481 atcgcggatt tgcgtctcga acagtttgaa gttaaccagc agcgtgatgc actcttccag
     6541 agcgatgcgt tcgtcaacaa actggaagaa ggtcacacca acgaagtcaa cagcgaagtt
     6601 cacgatgcgt tattgcaagt ggttgatatg cgtcgcgaat tgctggatca actcaacaaa
     6661 cagttgggta accagctgat gatggccatt aacctgcaaa tcaaccagca gcagttaatg
     6721 agcgtatcga aaaacctgaa atccatcctg actcagcaaa tcttttgggt gaacagtaac
     6781 cgtccaatgg actgggactg gatcaaagcg ttcccgcaaa gcctgaaaga tgaatttaag
     6841 tcgatgaaaa tcacggtgaa ctgggaaaaa gcctggcctg ccgtttttat cgctttcctc
     6901 gccggtttgc cgctgctgtt gattgccggg ctgatccact ggcgtctggg ctggctgaaa
     6961 gcgtatcaac agaaactggc ttccgctgtg ggttccctgc gtaacgatag ccagctcaac
     7021 acaccaaaag cgatccttat cgacctgatc cgcgcgctgc cggtgtgcct gattattctc
     7081 gcggttggcc tgattctgtt gaccatgcag ctcaacatca gcgaactgct atggtcgttc
     7141 agtaaaaaac tggcgatttt ctggctggtg tttggcctgt gctggaaggt actggagaaa
     7201 aatggcgttg ccgtgcgtca cttcggcatg ccggagcagc agaccagcca ctggcgtcgg
     7261 caaattgtcc gcatcagtct cgcgttgctg cctatccatt tctggtctgt ggtggcagaa
     7321 ctttccccgc tgcatctgat ggatgatgtg ctggggcagg cgatgatttt cttcaacctg
     7381 ctgctgattg ccttcctggt atggccaatg tgccgcgaaa gctggcgtga taaagagtcg
     7441 cacaccatgc gactggtcac cattaccgtg ctgtcgatta tcccgattgc attgatggtg
     7501 ctgactgcca cgggttattt ctacaccacg ctgcgcctct ccgggcgttg gattgaaacc
     7561 gtttatctgg tgatcatctg gaacttgctc taccagacgg tattgcgcgg cttaagcgta
     7621 gcggcgcggc gtatcgcctg gcgtcgtgcg ctggcgcgtc ggcagaatct ggtgaaagag
     7681 ggcgcagaag gtgccgaacc gccggaagaa cccaccattg cactggaaca ggttaaccag
     7741 cagacgctgc gtattaccat gttgctgatg tttgcgctgt tcggtgtcat gttctgggca
     7801 atttggtccg atttgatcac cgtgttcagc tatctcgaca gcatcacgct ctggcattac
     7861 aacggcactg aagctggcgc tgcggtggtg aaaaacgtca ccatgggcag tctgttgttt
     7921 gcgattatcg cctcaatggt ggcctgggcg ttgattcgca acctgcctgg tttactggaa
     7981 gtgctggtgc tctcgcgact gaatatgcgt cagggcgcgt cgtatgccat tactaccatc
     8041 cttaactaca tcattattgc cgttggtgcg atgacggtgt tcggatcgct gggcgtctct
     8101 tgggataaac tccagtggct ggccgcagca ttatccgtag gtcttggttt tggtttacaa
     8161 gaaattttcg gtaacttcgt ctccggtttg atcattctgt tcgaacgtcc ggtgcgtatt
     8221 ggcgatacgg taaccattgg tagcttctcg gggacggtaa gtaagatccg tattcgtgcg
     8281 acaacgatta ccgatttcga tcgcaaagaa gtgatcatcc cgaacaaagc gtttgttacc
     8341 gagcgtctga tcaactggtc gttgactgac actactacgc gtctggtgat ccgtctcggc
     8401 gtggcctatg gctccgatct ggaaaaagtg cgtaaagtgt tactgaaggc ggcgactgag
     8461 cacccaaggg tgatgcacga accaatgccg gaagtcttct ttacggcgtt tggtgccagc
     8521 acgttggatc atgagctgcg tctgtatgtg cgtgaactgc gtgaccgtag tcgtactgtc
     8581 gatgagctga accgtactat cgatcagctg tgccgtgaaa acgacatcaa cattgccttt
     8641 aaccagcttg aagtgcatct gcacaacgag aagggcgatg aggtgacgga agtcaaacgc
     8701 gactacaaag gcgatgaccc gacgccagcg gtagggtaat aaccaaaggg ggcgacattt
     8761 agttgccccg agattactga caaagtgcgc gttgttcatg ccggatgcgg cgtgaccgcc
     8821 ttatccggcc tacgaaaccg caagaattca atgtattgca ggagcggtgt aggcctgata
     8881 agcgtagcgc atcaggcagt tttgcgtttg cccacaatct taggggacat ttagcggccc
     8941 catttatttc tcacttttcc gcctcatcat cgcgcgttaa tttcttttca taatcgcgtt
     9001 ttacaatatc cagcgcgcgc agcacggtgc gggctgggat ctgattttct tccagcagca
     9061 caatcaaatc gacagccagt ttgacatcgt caggggcatt ttccagtgac atactctctc
     9121 cattgctagc gggttaagcg cgctagcctg ttttcgattt tttccagcgc atggcggcag
     9181 cgtgccaggc gcgcttcata ggcttccact tcacgatgca gcgtttgctg ttccacgaga
     9241 tcggtcaccc gcgccaggcg ggctctgcgt tcggcaacca tctcacgtag ccgccgctca
     9301 aaatcctgat gctgaatacg tttacgctgc cagcgggcaa ttttcggtgg cgcactgtcc
     9361 cactcgcgca atgaccaggc ggaggcttca cgcgcgatgg cttccagttg tgccgccaga
     9421 tgttccgcca gccaggccac ttgcggcagc tgttgctgct caactgcatg acgaagcgca
     9481 gccagattat cgcccgcctc atcgagacaa gcctgcagtg ttgtcgcacg ggtttgaaaa
     9541 aggtgtctgt caaaacgggc acttagcgtg gcaaattgcg ctaccggggc acaacgctga
     9601 cgcagcgtag cgagctgtcc ttccagtttt tccagcagca gggcggtttt cacgataaga
     9661 cctcaatgaa aatgataatt gttatgctaa agtagccact ctgtaagctg acaacactca
     9721 atatgcaacg aatcatttta atcatcattg gctggctggc ggtagtgctg ggtacgctgg
     9781 gcgtggtatt accggtatta ccgacgacgc cgtttatcct gctggcggcc tggtgctttg
     9841 cccgttcttc cccgcgcttt cacgcctggt tgctgtaccg ctcatggttt ggcagctatc
     9901 tacgtttctg gcagaaacat catgcgatgc cgcgcggcgt caaaccgcgg gcgattttgc
     9961 ttattttgct cacgtttgcc atttctctgt ggttcgtcca gatgccatgg gtgcgcatca
    10021 tgttgctggt aattctcgcc tgtttgcttt tctatatgtg gcgaattccg gtgattgatg
    10081 aaaagcaaga aaagcactga agcacaacaa tcgcagttgc aattattgcg tacagccagt
    10141 acattctggc gttttcgagc acaggcgcag gcggtcaaag gttaaacaac tgttactttt
    10201 gatacgttta aaacgcgccg tgagtaccac cgtaacaagc aggcatacac ttatgaccgc
    10261 gactgcacag cagcttgagt atctcaaaaa tagcatcaaa agcattcagg actacccaaa
    10321 acccggcatt cttttccgcg atgtcaccag cttactggaa gacccgaaag cttacgctct
    10381 cagcatcgac ttgctggttg agcgttacaa aaatgcgggc attaccaaag ttgtcggcac
    10441 cgaagcgcgt ggcttcttgt ttggcgctcc ggtagctctg ggtctgggcg ttggctttgt
    10501 accggtccgt aaaccgggca aactgccgcg tgaaaccatc agtgaaactt acgacctgga
    10561 atacggcacc gatcagctgg agatccacgt tgatgccatc aaaccgggcg acaaagttct
    10621 ggtggtggac gatctgctgg caaccggcgg cactatcgaa gcgaccgtta aactgatccg
    10681 tcgtctgggt ggtgaagtgg ctgacgctgc gttcattatc aacctgttcg atctcggcgg
    10741 cgaacagcgt ctcgaaaaac agggcattac cagctacagc cttgtcccgt tcccgggcca
    10801 ttaattatcg ccagtcttgt gctgcccacg ctacggacag cacaagatgt gcattcagcc
    10861 tcgccgttct gacggggctg tgttagcatt accccttcgt gaatccacct tccagcgttt
    10921 cagagcctgc caatgagtta tcaggtctta gcccgaaaat ggcgcccaca aacctttgct
    10981 gacgtcgtcg gccaggaaca tgtgctgacc gcactggcga acggcttgtc gttagggcgt
    11041 attcatcatg cttatctttt ttccggtacc cgtggcgtcg gaaaaacctc tatcgcccga
    11101 ctgctggcga aggggctaaa ctgcgaaacc ggcattaccg cgacgccgtg cggcgtgtgc
    11161 gataactgtc gtgaaatcga gcaggggcgc tttgtcgatc tgattgaaat cgacgccgcc
    11221 tcgcgcacca aagttgaaga tacccgcgac ctgctggata acgtccagta cgctccggcg
    11281 cgtggtcgtt tcaaagttta tctgatcgac gaagtgcata tgctgtcgcg ccacagcttt
    11341 aacgcactgt taaaaaccct tgaagagccg ccggagcacg ttaagtttct gctggcgacg
    11401 accgatccac agaaattgcc ggtgacgatt ttgtcgcgct gtttgcaatt tcatctcaag
    11461 gcgctggatg tcgagcaaat tcgccatcaa cttgagcaca tcctcaacga agaacatatc
    11521 gctcacgagc cgcgggcgtt gcaattgctg gcgcgcgccg ctgaaggcag cctgcgagat
    11581 gccttaagtc tgaccgacca ggcgattgcc agcggtgacg gccaggtttc aacccaggca
    11641 gtcagtgcga tgctgggtac gcttgacgac gatcaggcgc tgtcgctggt tgaagcgatg
    11701 gtcgaggcca acggcgagcg cgtaatggcg ctgattaatg aagccgctgc ccgtggtatc
    11761 gagtgggaag cgttgctggt ggaaatgctc ggcctgttgc atcgtattgc gatggtacaa
    11821 ctttcgcctg ctgcacttgg caacgacatg gccgccattg agctgcggat gcgtgaactg
    11881 gcgcgcacca taccgccgac ggatattcag ctttactatc agacgctgtt gattggtcgc
    11941 aaagaattac cgtatgcgcc ggaccgccgc atgggcgttg agatgacgct gctgcgcgcg
    12001 ctggcgttcc atccacgtat gcctctgcct gagccagaag tgccacgcca gtcctttgcg
    12061 cccgtcgcgc caacggcagt aatgacgcca acccaggtgc cgccgcaacc gcaatcagcg
    12121 ccgcagcagg caccgactgt accgctcccg gaaaccacca gccaggtgct ggcggcgcgc
    12181 cagcagttgc agcgcgtgca gggagcaacc aaagcaaaaa agagtgaacc ggcagccgct
    12241 acccgcgcgc ggccggtgaa taacgctgcg ctggaaagac tggcttcggt caccgatcgc
    12301 gttcaggcgc gtccggtgcc atcggcgctg gaaaaagcgc cagccaaaaa agaagcgtat
    12361 cgctggaagg ctaccactcc agtgatgcag caaaaagaag tggtcgccac gccgaaggcg
    12421 ctgaaaaaag cgctggaaca tgaaaaaacg ccggaactgg cggcgaagct ggcggcagaa
    12481 gccattgagc gcgacgcgtg ggcggcacag gtgagtcaac tttcgctacc aaaactggtc
    12541 gaacaggtgg ccttaaatgc ctggaaagag gagagcgaca acgcagtatg tctgcatttg
    12601 cgctcctctc agcggcattt gaacaaccgc ggtgcacagc aaaaactggc tgaagcgttg
    12661 agcacgttaa aaggttcaac ggttgaactg actatcgttg aagatgataa tcccgcggtg
    12721 cgtacgccgc tggagtggcg tcaggcgata tacgaagaaa aacttgcgca ggcgcgcgag
    12781 tccattattg cggataataa tattcagacc ctgcgtcggt tcttcgatgc ggagctggat
    12841 gaagaaagta tccgccccat ttgatcgtaa gcacagctta cgttcgtcat ccttaacgtg
    12901 attgagagag aaacctatgt ttggtaaagg cggtctgggt aacctgatga agcaagccca
    12961 gcagatgcaa gaaaaaatgc agaaaatgca ggaagagatc gcgcagctgg aagtcaccgg
    13021 cgaatctggc gcaggtctgg taaaagtgac catcaacggt gcacacaact gccgtcgcgt
    13081 agagatcgac ccgagcctgc tggaagacga caaagagatg ctggaagacc tggtggctgc
    13141 agcatttaac gacgcagcac gtcgtattga agaaacgcag aaagaaaaaa tggcctctgt
    13201 atcctccgga atgcagctgc cgcctggctt taagatgccg ttctgatgca aaccagcccg
    13261 ctgttaacac agcttatgga agcactgcgc tgtctgccgg gcgttggccc gaagtcggcg
    13321 cagcgtatgg cgttcacgct gcttcagcgc gatcgtagcg gcgggatgcg tctggcgcag
    13381 gcgctcaccc gggcgatgtc ggaaatcggc cactgcgccg attgccgcac tttcaccgaa
    13441 caggaagtct gtaacatctg ttcgaatccg cgtcgtcagg aaaacggtca aatctgtgtg
    13501 gtggagagtc cggcggacat ctacgccatt gagcagacgg ggcagttttc aggccgttat
    13561 tttgtgttga tggggcacct gtcaccgctg gacggcatcg gtccggatga tatcggcctt
    13621 gatcgtctgg aacagcgtct ggcagaggaa aaaatcactg aagtgatcct cgccaccaat
    13681 cccacggttg aaggtgaagc taccgctaac tacattgccg agctttgcgc gcaatatgac
    13741 gtggaagcca gccgaatcgc tcatggcgta ccggttggcg gcgaactgga aatggtcgat
    13801 ggcaccacgc tgtcacactc ccttgccggg cgtcataaga ttcgttttta agcaaacgag
    13861 agcaggatca cctgctctcg cttgaaatta ttctcccttg tccccatctc tccctcatcc
    13921 tgtttttaac cttaaaatgg cattattgag gtagacctac atgaaaggac aagaaactcg
    13981 tggttttcag tcagaagtga aacagcttct gcacctgatg atccattctc tctattccaa
    14041 taaagaaatc ttcctgcgtg agcttatctc taacgcctcc gatgcggcgg acaagctgcg
    14101 tttccgtgcg ctatctaacc cggacctgta cgaaggtgat ggcgaactgc gcgttcgtgt
    14161 ctctttcgat aaagacaagc gcacgctgac catctccgat aacggcgtgg ggatgacccg
    14221 cgacgaagtg attgaccatc tggggactat cgctaaatcc ggtaccaaat cattcctcga
    14281 atccctgggt tctgaccagg cgaaagacag ccagctgatc ggtcagtttg gtgttggttt
    14341 ctactctgcg tttatcgtgg ccgacaaagt gaccgtgcgt actcgcgcgg caggcgaaaa
    14401 accagaaaac ggcgtcttct gggaatcggc tggcgaaggt gaatataccg ttgccgacat
    14461 caccaaagaa gatcgtggta ctgaaatcac cctgcatctg cgtgaaggcg aagacgagtt
    14521 cctcgatgac tggcgcgtgc gttccatcat cagcaaatac tccgaccata tcgcgctgcc
    14581 ggtagagatc gaaaaacgcg aagagaaaga cggcgaaacc gttatctcct gggagaaaat
    14641 caacaaagcg caggcgctgt ggactcgtaa caagtcggaa atcaccgatg aagagtacaa
    14701 agagttctac aaacacatcg cccacgactt taatgatccg ctgacctgga gccacaaccg
    14761 cgttgaaggt aagcaggagt acaccagcct gctgtacatc ccgtcccagg ctccgtggga
    14821 tatgtggaac cgtgatcata aacacggcct gaaactgtac gtccagcgtg tgttcatcat
    14881 ggacgacgca gaacagttca tgccgaacta tctgcgcttc gtgcgtggtc tgattgactc
    14941 cagcgatctg ccgctgaacg tttcccgtga aatcctccag gacagcacgg taacgcgtaa
    15001 cctgcgcaat gcgctgacca agcgtgtgct gcaaatgctg gaaaaactgg cgaaagacga
    15061 cgcggaaaaa taccagacct tctggcaaca gtttggcctg gtactgaaag aaggtccggc
    15121 ggaagatttc gctaaccagg aagcgatcgc caaactgctg cgttttgctt ctacttatac
    15181 cgattcttct gcgcagaccg tgtctctgga agactacgtt tcccgcatga aagaagggca
    15241 ggagaaaatc tactacatca ctgcagacag ctatgcggca gcgaagagca gcccgcacct
    15301 ggaactgctg cgtaagaaag gcatcgaagt tctgctgctt tccgaccgca tcgatgagtg
    15361 gatgatgaac tatctgactg agttcgacgg taaaccgttc cagtcggtgt ctaaagttga
    15421 cgagtcgctg gaaaaactgg ctgacgaagt tgatgagagc gcgaaagaag cggagaaagc
    15481 actgactccg ttcatcgacc gtgtgaaagc cctgctcggc gagcgcgtga aagatgtccg
    15541 tctgactcac cgtctgaccg atacgccagc gatcgtttcg accgacgcgg acgaaatgag
    15601 cactcagatg gcgaaactgt tcgctgcggc gggccagaaa gtgccagaag tgaaatacat
    15661 cttcgaactg aacccggatc acgtactggt gaaacgtgcg gcagatactg aagatgaagc
    15721 gaagttcagc gagtgggtag aactgctgct ggatcaggcg ctgctggcag aacgcggcac
    15781 gctggaagat ccgaacctgt ttattcgtcg tatgaaccag ctgctggttt cctgatgtca
    15841 tgccggatga cctttgtgtc atccggcatt tttcttttca tcatctgcac tttccgcaaa
    15901 ttatctcgcc attaaccgtt tcagcctcag gtgcctttct tgaggcaatc gcctgttggt
    15961 ggtatcgttt atcgcttttt caaaaaattc gacacatttt aaggggattt tcgcaatgcg
    16021 tatcattctg cttggcgctc cgggcgcggg gaaagggact caggctcagt tcatcatgga
    16081 gaaatatggt attccgcaaa tctccactgg cgatatgctg cgtgctgcgg tcaaatctgg
    16141 ctccgagctg ggtaaacaag caaaagacat tatggatgct ggcaaactgg tcaccgacga
    16201 actggtgatc gcgctggtta aagagcgcat tgctcaggaa gactgccgta atggtttcct
    16261 gttggacggc ttcccgcgta ccattccgca ggcagacgcg atgaaagaag cgggcatcaa
    16321 tgttgattac gttctggaat tcgacgtacc ggacgaactg attgttgatc gtatcgtagg
    16381 ccgccgcgtt catgcgccgt ctggtcgtgt ttatcacgtt aaattcaatc cgccgaaagt
    16441 agaaggcaaa gacgacgtta ccggtgaaga actgactacc cgtaaagacg atcaggaaga
    16501 aaccgtgcgt aaacgtctgg ttgaatacca tcagatgact gcaccgctga tcggctacta
    16561 ctccaaagaa gcggaagcgg gtaacaccaa atacgcgaaa gttgacggca ccaagccggt
    16621 tgctgaagtt cgcgctgatc tagaaaaaat cctcggctaa ttcgaaagcg cgcacggaca
    16681 gtcccctcgc cccctcgggg agagggttag ggtgagggga acaggcccgc acaagcaaac
    16741 ttatcagcaa tctcaggccg gatattcatt cggcctttta caaaaacctc aatctgcgct
    16801 attccttttt ctgatttgac ctctcacagc aattagttct tcttcctcac ttttccgcta
    16861 caattatcaa caagttgaat cgataagagg cggtaatgcg tcagactaaa accggtatcc
    16921 tgctggcaaa cctgggtacg cccgatgccc ccacacctga agcggtaaaa cgctatctga
    16981 aacaattttt aagcgacaga cgcgtggttg atacctcacg gttgttatgg tggccgttgc
    17041 tgcgcggcgt gattttgccg ctgcgctcgc cgcgtgtggc gaagctgtac gcctcggtct
    17101 ggatggaaga cggctcgccg ctgatggttt acagccgcca gcaacagcag gcgttggcac
    17161 aacgtttacc ggatacgccc gtagcgctgg gcatgagcta cggctcgcca tcactggaaa
    17221 gcgccgtcga tgatctcctg gcagagcatg tagatcatat tgtggtgctg ccgctttatc
    17281 cgcaattctc ctgttctacg gtcggtgcgg tatgggatga actggcacgc attctggcgc
    17341 gcaaacgtag cattccgggg atatcgttta ttcgtgatta cgccgataac cacgattaca
    17401 ttaatgcact ggcgaacagc gtacgcgctt cttttgccaa acatggcgaa ccggatctgc
    17461 tgctgctctc ttatcatggc attccccagc gttatgcaga tgaaggcgat gattacccgc
    17521 aacgttgccg cacaacgact cgcgaactgg cttccgcact ggggatggca ccggaaaaag
    17581 tgatgatgac ctttcagtcg cgctttggcc gggaaccctg gctgatgcct tataccgacg
    17641 aaacgctgaa aatgctcgga gaaaaaggcg taggtcatat acaggtgatg tgcccgggct
    17701 ttgctgcgga ttgtctggag acgctggaag agattgccga gcaaaaccgt gaggtcttcc
    17761 tcggtgccgg cgggaaaaaa tatgaatata ttccagcgct taatgccacg ccggaacata
    17821 tcgaaatgat ggctaatctt gttgccgcgt atcgctaaag ctgagcggta aagaactgag
    17881 cgccgtcgcg aagagcctcg tcggcggttt tcatcattcg tgaataatgc aaaaaggcat
    17941 gcagcgtacc tgggtagagt ttgaactcac agggctgctg atgcgccgct aacgtctggt
    18001 aaagcagacg gctgtcatcc agcagcggat cgaactccgc cccggcaata aaacagggcg
    18061 gaacttcgcg agtgagatca ttattaaaca gacagtaata cggtgactcg cggtccgcgt
    18121 cgttgcttaa atacgcctct tcatacatct gcaaatcctg ttgcgttaag ccatcccaga
    18181 caccgcccaa cagacgacga gtcacggaat cccgtaatcc gtaaagccca taccacagca
    18241 aaacacccgc aattttaccg caatcgatct gtttatcacg caaccacaac gcactggcga
    18301 gcgccagcat ggcacctgcg gaatcaccgg caaagccaat gcgggacata ttgatttgat
    18361 aatcctccgc ctgctggtgg aaataacaac aagcagccac aatttcctct atcgcttgcg
    18421 gaaaacgcgc ttcaggtgaa agggggtaat caataccaat caccgtacat tggctgtagc
    18481 ttgccagcag gcgcatgatg cgatcgtggg tatcgagatt gccgagaata aaaccgcctc
    18541 catgcaaata aaatagcgtc gccgggctat ctggctgcgg acaaaagaga cgtgtttcca
    18601 cctgcccata ttttgttgga accatgtaag ctctggttgc catttctgga gcgcccgcat
    18661 tccagaatcg gcgctcaagc gtgtaatact gtcgttgctc agcaatcgtt cccgttgcgg
    18721 gccaggacgg taaatccgac tgaagagtat tcacaacggt cttcatttca gcagaaataa
    18781 ggtccagaac aggtagtttg ttttccggct tcatacaaaa actcctttca aattacgtca
    18841 ttgtaaggaa ccactgccat gaaaatgcga tcccgcctgc tgatattgaa actggctgcg
    18901 tctcgcgcgc tcccgtcaga ttgtgttaac attcgccgct cagttaacca cccgtaaaaa
    18961 caaccatgaa atttcccggt aaacgtaaat ccaaacatta cttccccgta aatgcacgcg
    19021 atccgctgct tcagcaattc cagccagaaa acgaaaccag cgctgcctgg gtagtgggta
    19081 tcgatcaaac gctggtcgat attgaagcga aagtggatga tgaattcatt gagcgttatg
    19141 gattaagcgc cgggcattca ctggtgattg aggatgatgt agccgaagcg ctttatcagg
    19201 aactaaaaca gaaaaacctg attacccatc agtttgcggg tggcaccatt ggtaacacca
    19261 tgcataacta ctcggtgctc gcggacgacc gttcggtgct gctgggcgtc atgtgcagca
    19321 atattgaaat tggcagctat gcctatcgtt acctgtgtaa cacctccagc cgtaccgatc
    19381 ttaactatct acaaggcgtg gatggcccga ttggtcgttg ctttacgctg attggcgagt
    19441 ccggggaacg tacctttgct atcagccctg gccacatgaa ccagctgcgg gctgaaagta
    19501 ttccggaaga tgtgattgcc ggagcctcgg ctctggttct cacctcttat ctggtgcgtt
    19561 gcaagccggg tgaacccatg ccggaagcaa ccatgaaagc cattgagtac gcgaagaaat
    19621 ataacgtacc ggtggtgctg acgctgggaa ctaagtttgt cattgccgag aatccgcagt
    19681 ggtggcagca attcctcaaa gaccacgtct ctatccttgc gatgaacgaa gatgaagccg
    19741 aagcgttgac cggagaaagc gatccgttgt tggcatctga caaggcgctg gactgggtag
    19801 atctggtgct gtgcaccgcc gggccaatcg gcttgtatat ggcgggcttt accgaagacg
    19861 aagcgaaacg taaaacccag catccgctgc tgccgggcgc tatagcggaa ttcaaccagt
    19921 atgagtttag ccgcgccatg cgccacaagg attgccagaa tccgctgcgt gtatattcgc
    19981 acattgcgcc gtacatgggc gggccggaaa aaatcatgaa cactaatgga gcgggggatg
    20041 gcgcattggc agcgttgctg catgacatta ccgccaacag ctaccatcgt agcaacgtac
    20101 caaactccag caaacataaa ttcacctggt taacttattc atcgttagcg caggtgtgta
    20161 aatatgctaa ccgtgtgagc tatcaggtac tgaaccagca ttcacctcgt ttaacgcgcg
    20221 gcttgccgga gcgtgaagac agcctggaag agtcttactg ggatcgttaa gttatcgtcg
    20281 gttcgtaggc cagataaggc gttcacgccg catctggcat ttggctctcg atgcccgatg
    20341 cgacgctggc gcgtcttatc atgcctacat atttttcata ttttacatcc ggcaaccacc
    20401 gtttaccccg tcaccacttc acccgccggt ggcgtttcca gcagttccag catagtacgg
    20461 gcaatttcac gttcacccat cactacctga ttcgcgccac gttcggtgat atacgtcact
    20521 tcatcgtcat aatgggcgcg ggcgataatc tcaatatccg ggtttttcgc gcgggcagat
    20581 gccacaatct cgcctgcttc ataaccgttg ggaatcgtca ggatcagcca ttttgcacat
    20641 tccagatgcg ccagttgcat aatttcttcg ttcgccgcat tgcccaatac tgcgcggacc
    20701 ccgcgctctc gcagctcatc aacacgggtt cgtgacgtct caatcaccac cagcggaata
    20761 tcagaggcga gcaatttctc ccccagcagg ctgcctacac gaccgtaacc caccagtagc
    20821 gcatggttgc aaatatccac tgggatctgc ttctcttctt cgattgcctc ttccagcgtc
    20881 tgctcttcca gcgtttcggt cttcgccaga tatttctcca gtagtgcgaa cagtaccggg
    20941 ttgagcataa tcgacaggat cgcccctgcc agtaccaggt tttgtccggc ctgcggcagt
    21001 aaattcaatg ccattcccag tcccgccagg ataaacgcga actcaccaat ctgcgccagg
    21061 ctggcggcga tggttaatgc cgtacgttgg gagtgaccaa acagtcgcac caggaaaaat
    21121 gcggctaacg acttaccaaa cagaataatc gccagcgtcg ccagcactgc cagcggttgc
    21181 tgaatcagaa ttaacggatc aaacaacatc ccgacggaga caaaaaacag caccgcaaac
    21241 gcgtcgcgca atggcagcgt atcgtgggcg gcacggtgac tcagttcaga ctcgttcagt
    21301 accatcccgg caaagaacgc accgagtgca aaggagacat caaacagctc taccgcacca
    21361 aaggcaatcc ctaacgccag cgccagcacc gacagggtaa acagctcgcg agaaccggtt
    21421 gccgcgctgc gtgccataat ccacggcacc agacggcgac ctaccagcat cataatggcg
    21481 ataaatgcga tcactttgcc gatggtgatc cccatatcga ctgcaagagt ggcaaagccc
    21541 acatcgccct gttccatcat tcctgccact gcgggcagca acaccagcgt cagaaccatt
    21601 accaggtctt ccacaatcaa ccaaccgatg gcgatttgcc cacgctgact gtcaattaat
    21661 tgccgttctt caagtgcgcg cagtaacacc acggtactgg cggtggaaag acataaaccg
    21721 aacacgatac cggtcattaa cgaccagccc agcacggcag agagcgccat acccagcagc
    21781 gtcgccacgg ctatctgggc gatcgcaccg ggaatggcga tggcctttac cgccatcaaa
    21841 tccttcagcg aaaaatgcaa accgacgccg aacatcagca aaataacgcc cagttcagcc
    21901 agttccggcg caagcttggt atcggcaaca aagcccggag tgaatggtcc tgccagcaca
    21961 cccgctaaca gatatcccac cagaggagaa atacgtagtt tattggccag catgccgagg
    22021 ataaaggcga gcacaaggcc gccaacaatg gtggtgataa gcggggtggc gtgatgcatt
    22081 ccgtctcctt ttcctggtgg ttattgtcca tttttggccg gggaaaccaa aattacaggt
    22141 aatagtttat gacaatttca ttgatgatgt tcatgaataa ttattgaatt ttgcagaaaa
    22201 atggaattag ctgcaaaaaa agcacggatc ggaaaacaga aggcggttta acagaggaaa
    22261 ggcttatggc gactgtaaaa cgatgcagcc aaagtttgcc tttggctgca atgaaatcag
    22321 tctttatgcc ggttatcagg caggaatatg gtcaacatcc ccaatagtgg caggaaagca
    22381 cagattttat agactaactc gatgctggtg tgatcggcga taagccccag aactgccgct
    22441 cccagacctc ccatgccaaa agcaaaaccg aaaaagagtc cagaaaccat accgatacgt
    22501 cccggaagta gctcctgagc gtagaccaga atggcagaga atgccgaagc gaggataaat
    22561 ccaataatca ccgttaaaac ccccgtccag tgcaggctgg cgtagggtaa aatcagcgta
    22621 aacggcgcaa cgccgaggat agagccccaa atcacatatt tccgcccaat tttatcccct
    22681 acaggcccgc cgatcaccgt acctgccgca acggcaaaca ggaaggcaaa cagatgaagc
    22741 tgagcattct ggatagataa tccgaatttt tgcatcagat aaaaggtgta atagctgctg
    22801 atgctcgcca tatagaaata tttcgagaaa atgaggatta acagaatgct gaccgccagt
    22861 accactttgt tgcgcggcag tggattgata atcgtcgctt tgggttttcc tttattcatt
    22921 cggtgctgtg ccgagtacca acggctaatt tgcgccaaca ccacgatcgc cagcagtgcc
    22981 gcaagcacaa accaggcaac gttgcctttg ccataaggcg cgataatcac cgccgccagc
    23041 aagggtccca gggaactgcc aaagttgccg ccgacctgaa agatagattg cgccaggcca
    23101 tgccacccgc cggaagccat acgggctacg cgagaagatt ccggatgaaa gaccgatgaa
    23161 ccggtaccga ccagcgccgc cgccagcaga actgcgccaa aactgcccgc cagcgcaagc
    23221 agcaccagac cacttaaggt aaagcacatg ccaattggca acgaccacgg catcggatat
    23281 ttatcggtcc agtagccgac cactggttgc agtagcgaag aggcgagctg gaaggtgagg
    23341 gttatcatgc caatctgcat aaatgtcaga gaaaattctg actgaagcag cggataaatc
    23401 gccagaatca gcgattggat catgtcgttc agcagatgtg agaggctgat agcacctaaa
    23461 ataccaaacg atgttcgggc cttggtcgtt gacgcagccg cgcccgccac aggctggggt
    23521 tgttcactca ttgccatagg aaagtcactt tttcagggtt gcgatgtaaa gaatgatctt
    23581 atttgtgatt attacccgac taacatacct gtatgcgtcg tctgaaggaa gtctcaacgc
    23641 cgaatacaga atttctaatc tggatgcaga tttatcttca ccggacgcag acttgtctat
    23701 gatgtcgcgt catactattt ttcaacacgt tgaaatcagg tcagggagag aagtatgaaa
    23761 ttattgcagc ggggcgtggc gttagcgctg ttaaccacat ttacactggc gagtgaaact
    23821 gctctggcgt atgagcagga taaaacctac aaaattacag ttctgcatac caatgatcat
    23881 catgggcatt tttggcgcaa tgaatatggc gaatatggtc tggcggcgca aaaaacgctg
    23941 gtggatggta tccgcaaaga ggttgcggct gaaggcggta gcgtgctgct actttccggt
    24001 ggcgacatta acactggcgt gcccgagtct gacttacagg atgccgaacc tgattttcgc
    24061 ggtatgaatc tggtgggcta tgacgcgatg gcgatcggta atcatgaatt tgataatccg
    24121 ctcaccgtat tacgccagca ggaaaagtgg gccaagttcc cgttgctttc cgcgaatatc
    24181 taccagaaaa gtactggcga gcgcctgttt aaaccgtggg cgctgtttaa gcgtcaggat
    24241 ctgaaaattg ccgttattgg gctgacaacc gatgacacag caaaaattgg taacccggaa
    24301 tacttcactg atatcgaatt tcgtaagccc gccgatgaag cgaagctggt gattcaggag
    24361 ctgcaacaga cagaaaagcc agacattatt atcgcggcga cccatatggg gcattacgat
    24421 aatggtgagc acggctctaa cgcaccgggc gatgtggaga tggcacgcgc gctgcctgcc
    24481 ggatcgctgg cgatgatcgt cggtggtcac tcgcaagatc cggtctgcat ggcggcagaa
    24541 aacaaaaaac aggtcgatta cgtgccgggt acgccatgca aaccagatca acaaaacggc
    24601 atctggattg tgcaggcgca tgagtggggc aaatacgtgg gacgggctga ttttgagttt
    24661 cgtaatggcg aaatgaaaat ggttaactac cagctgattc cggtgaacct gaagaagaaa
    24721 gtgacctggg aagacgggaa aagcgagcgc gtgctttaca ctcctgaaat cgctgaaaac
    24781 cagcaaatga tctcgctgtt atcaccgttc cagaacaaag gcaaagcgca gctggaagtg
    24841 aaaataggcg aaaccaatgg tcgtctggaa ggcgatcgtg acaaagtgcg ttttgtacag
    24901 accaatatgg ggcggttgat tctggcagcc caaatggatc gcactggtgc cgactttgcg
    24961 gtgatgagcg gaggcggaat tcgtgattct atcgaagcag gcgatatcag ctataaaaac
    25021 gtgctgaaag tgcagccatt cggcaatgtg gtggtgtatg ccgacatgac cggtaaagag
    25081 gtgattgatt acctgaccgc cgtcgcgcag atgaagccag attcaggtgc ctacccgcaa
    25141 tttgccaacg ttagctttgt ggcgaaagac ggcaaactga acgaccttaa aatcaaaggc
    25201 gaaccggtcg atccggcgaa aacttaccgt atggcgacat taaacttcaa tgccaccggc
    25261 ggtgatggat atccgcgcct tgataacaaa ccgggctatg tgaataccgg ctttattgat
    25321 gccgaagtgc tgaaagcgta tatccagaaa agctcgccgc tggatgtgag tgtttatgaa
    25381 ccgaaaggtg aggtgagctg gcagtaatcc gaaagtgccg gatgtttgca tccggcacaa
    25441 tgcttaatcg cggcgggcga tatcagcaaa tttggcatcg aggatctttg ccagatcgcc
    25501 tgccgccagt tcgatatcca gtccgcgctt gccgccggaa acataaatag tggcaaattc
    25561 ttgtgcgggg gcgtcgataa tcgttggcag acgttttttc tgccccagtg ggctaatccc
    25621 cccaaccagg tatcccgtcg aacgctgcgc gaccatcgga tcggccatct caactttctt
    25681 ggcacccagc gcttttgcta cttttttaag atccagttga cctgcgaccg gcgtaacggc
    25741 cacggcaagg tgtttcatat caccgttcac tgccaccagc agcgttttgt agacctgatc
    25801 cggattcaaa cctaattttt tgacgacttc atcgccaaaa ttggtttcag ccggatcgtg
    25861 ctcgtaggta tggatttgaa acgaaatctt gtttttttcg agtaatttaa ctgcgggtgt
    25921 catggtaatt ttcctgcctt aaacaaaaaa acgaagcaag catacgcctg atggcggtcc
    25981 aaaaaaaaga gtcatcttgc ctaagagtat tggcaggatg gtgagattga gcgacaatcg
    26041 agttacaccg tcgcttaaag tgacggcata ataataaaaa aatgaaattc ctctttgacg
    26101 ggccaatagc gatattggcc atttttttag cgcaacattt gcggcaaatt cccttctcca
    26161 tacaggtgta gtgcaccgac cgcgaccaca tatcgccccg gcggcatggc gcgtaattta
    26221 tcccgccagg cgagatttcg ctgatgcatc agcacatcgt acagcgactg actgaacgta
    26281 ttgggcagcg ttatttcatt attttgcggc ggcgcattca gccaccagct catcatttgt
    26341 tgcagcaacc gtgcgttggt atgccagtgg gtcagcgtat cgtccagcag cgccagtcct
    26401 ttgtcaggga gctggagcaa catggcaatc tggttttcag ccccttccag ttcaatcacg
    26461 ggtttatgtt gttgcttcgc cgcctgcaat agctggtaat cgataccgta ttctgcccgc
    26521 agccccagtt tttgtgcctg cgtcgcctga agaaccatcg cgatttgcca cagcggttgg
    26581 gtagaaaaga gtgagggaga aatgcccatc tcctggctaa tgtgctgcag attttggagt
    26641 tgctcctcgc taatgcgctc ttccagcgcc tcgcaggcag gcaaattagc aaaaggcgta
    26701 tcgctggtgg aaacatccgc ctcgacgatc agcgcatcgg cgtttttgag ctttttgagc
    26761 aaacgggtgg gcaggggagc catatcgtgg ctacccatat gaatactgcc aatcagatga
    26821 aaatggcgat tgccggggag ggtgatatcg atggcaggcc aggtgtaatg attaccgcgc
    26881 agcgcggccc aaagcgtttt tacccggtac aacagatcca tacgacctcc ctttgtgaaa
    26941 tatcatgcta gcgcgcggtg agggatggcg caaatggagt tggggacttg tttaatgtgt
    27001 ttgtatgatt cagtatgttc ttgcatcgct attcacaggg aagcaacagt taaaaaccat
    27061 gaaacaggca acaagaaaac cgacgacacc cggagatatt cttctctatg aatatctgga
    27121 accgctcgat ttgaaaatca atgagttagc agagttgctg catgttcatc gtaatagcgt
    27181 cagtgcactg atcaataaca atcgtaaact caccactgag atggcattcc gtctggcgaa
    27241 agtttttgat accacagtcg attttaggct aaacctccag gcggcagttg atctttggga
    27301 agttgaaaac aacatgcgca cccaggaaga attgggacgg attgaaacag tggctgaata
    27361 tttggcacgc cgtgaagagc gtgcaaaaaa ggtcgcgtaa ggccaaaaca tcgcttcatc
    27421 tcgtaggccg gataaggcgt ttacgccgca tccggcatcc gagcaccatt gcctgatgcg
    27481 acgcttaccc gtcttatcag gcctacaaac ctgtgcctga accgtaggcc ggataaggcg
    27541 tttacgccgc atccggcagt catgcgtcga tgccaaatgc gccgccctaa agcagcgcat
    27601 ccgcaatgac gtacttattc cttcggttta aaccgcagca accggttggc gttactcact
    27661 acggtaatcg acgagagcgc cattgccgct ccggcaacta ccgggttaag cagtgttcca
    27721 gtaaacggcc acaaaatacc ggcggcgacc ggaataccga tgctgttgta gataaaggca
    27781 ccgagcaggt tctgcttcat gttgcgcagc gttgcgcggg aaatcgcgag cgcatccgca
    27841 acgcccatca ggctatggcg catcagggta atcgccgcgg tttcaatggc aacatcactg
    27901 ccgccaccca tcgcaatgcc gacatccgcc tgggccagcg ctggcgcgtc gttaatgccg
    27961 tcgcccacca ttgccacctg acgtccttca ctttgcagac gtttgatcgc ttcggcttta
    28021 ccgtccggca gcactccggc aatcacttca tcaattccag cttctttggc gatcgcattg
    28081 gcggtggttg ggttatcccc ggtcaacatc accagacgat atcccgcttt atgcaggcgt
    28141 tgcagcgccg ccacgctatc actacgcaac ggatcgcgta cagccagcag ggctactgct
    28201 ttcccgtcaa tcgccagcag cacaggcgtt gctccttgcg atgcctgagc agtaatctcc
    28261 gcttcgatag ctttggtacc aacctgttgc tcatttaaca gcgcctgatt gcccagcaat
    28321 aacgcatgac cttcagcttc accgctcacg cccagcccgc gcaatgtgcg gaaaccgttg
    28381 acctgcggca gctgcatatc acctgcttta tcgaggatcg cccgtgccag cggatggctg
    28441 gaaccttgct ccagtgccgc cgccagacgc aatgcctgcg cttcatcaac atcagcaaac
    28501 gttttcactg cgacaacctg cggcttccct tcggtcagcg tcccggtttt atcgaacacg
    28561 acggtatcga gtgtactggc gcgttgcagc gcgtcggcgt cccgcaccag cacgccaaac
    28621 tcagccgccc gcccgacgcc ggaaataatc gacatcggcg tcgccagccc cagcgcacac
    28681 ggacaggcga taatcagtac cgtggtggca atcaccaggg tataaacaat ttgcggtgcc
    28741 ggaccaaaga agtaccagat tgccgcactg accagcgcaa tcaccaccac cacaggtaca
    28801 aatacggctg agattttatc cgccagctga ccgatttctg gcttgctgct ctgggcctgg
    28861 cgcaccatgc gaatgatccg tgacagcgtg gtatggctgc caaccgcact ggcacgaaac
    28921 agcacactgc cgtcctgtac cactgtcccg gcatggacgc tttcgccttc gcctttttgc
    28981 tgtgggattg gttcgcccgt cagcatcgct tcatccagcc atgcttcgcc ctgggtaatc
    29041 tcgccatcta ccggcacgcg atcgccggtc gtcaggcgca gcaacatacc tggctgcact
    29101 tccgccagag gcacgctttt ttcaccttcg tcagtaacca ggcgcgccgt cggtggggtt
    29161 aaatcgagta atttctccag cgccttagaa gaacgctgac gtgcgcgcgc ttccagcata
    29221 tggccgagat taatcagacc gataatcatc gcactggctt catagtaaag atgccgcgct
    29281 tccatcggga accactgcgg ccacaggttg acgctcatcg aatagagcca cgccacaccc
    29341 gttcccagcg ccaccagcgt atccatcgtc gctgcaccgt tcagcaggct tttccatgca
    29401 ctgcggtaaa aatggccgcc ggcgaaaacc atcaccgcca gggttatcag gccgataacc
    29461 aaccacaggc tgcggttgtc agcggtgacc atcatgttat cgccaatcat cccccagacc
    29521 atcaccggga tacccaccgc cagtgcgaca attgcctgcc agcggaagcg cttcatcgta
    29581 gcaacggcgg tttcctgctg gcgctcacgg cgtttcgcgt catcttcaat cgcttccgcg
    29641 ccgtagcccg ctttttccac cgcctgcact aaatcttgtg gggaggcact gcccatcacc
    29701 agcgcagtac gctccgccag gtttacccgt gcctgagtga cgcccggtac gctttgcagc
    29761 gcattttgta cgcgggtgac acagctggcg cagctcatgc cgctcagcag caactgctgg
    29821 ctgtcatcgt catcggcggt cgctgccgga agcgcctcag aaaccgctgt cagtgcttcc
    29881 gacgggattg atgactccgc cagcggttta gcctttgggt ggcttacaga tgcgtcataa
    29941 cccgcttgtt tgatggtttc gatcagctgt tctgcactgg cagtcccggt aacgtgcgct
    30001 tcagtgatag acacatccgc ttgttccaca tccgggcgct gttcgagact ttctttcacg
    30061 cgtttaacgc agtgaccgca ggacaggccg tccagggtca ggtcgataat ttgtgacata
    30121 aaacactcct ttaagacagt tttgactggc tgtgataaag gttaaacctt ccagcaaggg
    30181 gaaggtcaag aaattaataa accaggcgag taaaagtccg taaagattaa aaaattggct
    30241 caatttgcat caggattaga catttatctc tttgttttcc tgtagttaag ttgcgggtgc
    30301 taagttaaat caggatgcct caaaatcggc accggggtga ggaattacct cccgcatcta
    30361 taaaaaggag ttaacaaaag atgttagatg caaacaaatt acagcaggca gtggatcagg
    30421 cttacaccca atttcactca cttaacggcg gtcaaaatgc cgactacatt ccctttctgg
    30481 cgaatgtgcc tggtcaactg gcggcagtgg ctatcgtgac ctgcgatggc aaggtctata
    30541 gtgcgggtga cagtgattac cgctttgcgc tggaatccat ctcgaaagtc tgtacgttag
    30601 cccttgcgtt agaggatgtc ggcccgcagg cggtacagga caaaattggc gctgacccga
    30661 ccggattgcc ctttaactca gttatcgcct tagagttgca tggcggcaaa ccgctttcgc
    30721 cactggtaaa tgctggcgcc attgccacca ccagcctgat taacgctgaa aatgttgaac
    30781 aacgctggca gcgaatttta catatccaac agcaactggc tggtgagcag gtagcgctct
    30841 ctgacgaagt caaccagtcg gaacaaacaa ccaacttcca taaccgggcc attgcctggc
    30901 tgctgtactc cgccggatat ctctattgtg atgcaatgga agcctgtgac gtgtacaccc
    30961 gtcagtgctc cacgctcctc aatactgttg aactggcaac acttggcgcg acgctggcgg
    31021 cggggggagt gaatccgctg acgcataaac gcgttcttca ggccgataac gtgccgtaca
    31081 ttctggccga aatgatgatg gaagggctgt atggtcgctc cggtgactgg gcatatcgcg
    31141 ttggtttacc gggcaaaagc ggtgtaggtg gcggtattct ggcggtcgtc cctggtgtga
    31201 tgggaattgc cgcgttctca ccaccgctgg acgaagaagg caacagtgtt cgcggtcaaa
    31261 aaatggtggc atcggtcgct aagcaactcg gctataacgt gtttaagggc tgatcatgat
    31321 gaacacggaa ggtaataacg gtaacaaacc cctcggtcta tggaacgtcg tttccatcgg
    31381 cattggggca atggtggggg cggggatctt cgcgctgctg gggcaggctg cgttgctgat
    31441 ggaagcctcg acctgggtcg cctttgcttt tggcggtatt gtggcgatgt tttccggtta
    31501 tgcctatgcg cgtctggggg cgagctatcc cagcaatggc ggcattatcg acttctttcg
    31561 tcgcggatta ggcaacggcg tcttttcgct ggcgctctcg ttactgtacc tgttgacgct
    31621 ggcggtgagc atcgccatgg tcgcccgtgc ttttggcgct tatgccgtgc agtttttgca
    31681 tgaaggcagc caggaggagc accttatttt gctctacgcg ttggggatca ttgcggtgat
    31741 gacgcttttc aactccttaa gcaaccatgc ggtagggcgg ctggaagtga tcctcgtcgg
    31801 cattaaaatg atgatcctgt tattgctgat tattgccggt gtctggtcgc tgcaaccggc
    31861 gcatatttcc gtctctgcgc cccccagctc cggtgcgttc ttctcctgta tcgggataac
    31921 cttccttgcc tatgcgggct ttggcatgat ggcgaacgcg gcggataaag tgaaagatcc
    31981 gcaggtcatt atgccgcggg cgtttctggt ggcgattggc gttaccacgt tgctttatat
    32041 ctcgctggca ctggttttgc ttagcgatgt atcggcatta gagttagaaa aatatgccga
    32101 taccgccgta gcgcaggctg cttctccgct gctcggacat gtgggttatg tgatcgtcgt
    32161 catcggcgct ttactggcga cggcttcagc cattaacgcg aacctgttcg ccgtgtttaa
    32221 catcatggac aacatgggca gcgaacgcga actgccgaag ctaatgaata aatccctgtg
    32281 gcggcagagt acctggggca acatcattgt cgtggtgttg attatgctga tgacggcggc
    32341 gctaaattta ggctcactcg ccagtgttgc cagcgccacc tttttgattt gctacctggc
    32401 ggtgtttgtg gtggcgatcc gcctgcgtca tgatattcac gcctcgttgc cgattcttat
    32461 cgttggtacg ttggtgatgt tgttggtgat cgttggcttt atctacagtc tgtggtccca
    32521 ggggagccgt gcgttgatat ggattattgg ctcactctta ctcagcctta ttgtggcaat
    32581 ggtcatgaag cgcaataaaa ccgtataaca tctctctgtg cgcagtactt cctgtattat
    32641 tgtggtggcg gtcgatattc gcactggcaa aaaaacgtgc ttgaatatct gttgaaaccc
    32701 tttaacaaag cacaggaggc gttgcgcgaa cgatgaacat cagcgatgta gcaaaaatta
    32761 ccggcctgac tagcaaagcc attcgcttct atgaagagaa ggggctggtg acgccgccga
    32821 tgcgcagcga aaacggttat cgcacctaca cgcagcagca tctcaacgaa ctgaccttac
    32881 tgcgccaggc acggcaggtg ggctttaacc tggaagagag cggcgagctg gtgaatctgt
    32941 ttaacgaccc gcagcggcac agcgccgacg tcaaacggcg cacgctggag aaggtggcgg
    33001 agatcgaacg acacattgag gagctgcaat ccatgcgcga ccagctgctg gcactggcga
    33061 atgcctgccc tggcgatgac agcgccgact gcccgattat cgaaaatctc tccggctgct
    33121 gtcatcatcg ggcagggtga ttaagacgag acggctcgga tatgtagggt tatcccttct
    33181 atcgcaatga cttcaacatg cgtacctgca ccgagatcct cgctggcgct gacaggccat
    33241 gaactgtcac cgacgcgcat atgaccgcgc ccgttgacca gcggagattc cagcacaaaa
    33301 cgtcggccaa tcagctgctg cccgcgctgg tttaaatgac tatcgctgtg cttttgttcg
    33361 cgcacccgcc gcgacaacca tttccaccac agccaggcgg cgagcagcgt caggatggca
    33421 aacattaccc cttgccactc ccaacccagc ggcaccagcc agaccaccag gccggtaatc
    33481 accgctgcca cgccgctcca caacaaataa ccatttccgc ccagcatctc ggctgccagc
    33541 agcaaaccgc cgagactgag ccagaaaata tgtggatgaa cgaccattaa ctccatcatg
    33601 gctgagtccg tttgttggcg ctgtctttca ccagctcggc aatcccggca atcgacccca
    33661 tcaggctgct ggcctctaat ggcatcatca ccactttgct gttactggac gaaccgatct
    33721 gctgtaacgc ttcggtgtat ttctgcgcta cgaagtagtt caccgcctga atatcacctg
    33781 aggcgatggc ttcagacacc attttggtgg cgcgggcttc tgcttcagcg gaacgttcac
    33841 gcgcttcagc ctgtaaaaac gccgactgac gttcgccttc cgctttcagg atttgcgact
    33901 gtttttcacc ttcggctttg aggatttccg cctgacggat cccttccgct tcaaggatgt
    33961 aagcgcgttt ggtacgttcc gctttcatct gcgcgttcat tgaagagata agctctgccg
    34021 gtgggcgcac gtcgcgaatt tcaatacggg tgactttaat cccccacggg ttggtggctt
    34081 catcgacaat acgcagcagg cgtgagttga tgctgtcgcg ctgagagagc atttcatcaa
    34141 gttccattga acccagcacg gtacggatgt tagtcatggt caggttgatg atagccagct
    34201 ccagattgct gacctcataa gccgcgcgtg gcgcgtcaat cacctgaata aagcacacgg
    34261 cgtcgatggt aacgttggcg ttatctttcg agataacttc ctgggaaggg atatcgagca
    34321 cttgctccat catattgatc ttgcgaccaa tgcgatccat aaacggcacc accagactga
    34381 gccccggctg taacgttttg gtatagcgac caaaacgttc taccgtccat tgatagccct
    34441 gcggcacgat tttgacgccc gcgccgacaa tgaccagcgc gacaaaaatg agaatcggga
    34501 taaagataag catcggaaaa acctcctgtt gtaccgtcca taatcagcaa aattgctgct
    34561 tgattaaaca aattatacct gattactgaa agagagttcc cccttattcc tgcgaaggat
    34621 aaactgtttt tagtaaaaat cagaaaaagg gaacagcgat gcaggaaaat agtcctttgc
    34681 ttcagctaca aaacgtagga tatctggcgg gtgatacgaa gattcttaat aacatcaatt
    34741 tttcgctgcg tgctggcgaa tttaagttaa ttaccggtcc ttctggttgt ggcaaaagta
    34801 cgctgctaaa aatagttgct tcattgatca gcccaaccag cggaacgtta ctgtttgaag
    34861 gggaggatgt cagcacacta aagccagaaa tctaccgcca gcaagtctct tactgcgccc
    34921 agacaccgac gctgtttggc gatacggtat acgataatct gatctttccc tggcagatcc
    34981 gtaaccagca gcctgacccg gccatttttc tcgattttct cgaacgcttc gctttgccgg
    35041 acagcatttt gacgaaggat atcgccgagc tatctggtgg tgaaaaacaa cgcatctcat
    35101 tgattcgtaa cctgcaattt atgccgaagg ttttattgct ggatgaaata accagtgcgc
    35161 tggatgaaag taataaacat aacgtcaatg agatgatcca tcgttatgtg cgcgagcaaa
    35221 atattgccgt gctgtgggtg acacacgata aagacgaaat taatcatgcg gataaagtga
    35281 ttacactgca accgcatgcc ggagaaatgc aggaagcacg ctatgaactc gcataatatt
    35341 actaacgaat cattagcact ggcattaatg ctggtggtgg tggcaatctt aattagccat
    35401 aaagaaaaac tggcgctgga gaaagatatt ctctggagcg tcgggcgagc gataattcag
    35461 ctgattattg tcggctatgt gctgaagtat attttcagcg tggatgatgc cagcctgaca
    35521 ttattgatgg tgttatttat ctgctttaat gcggcgtgga acgcgcaaaa acgcagtaaa
    35581 tatattgcta aagcttttat ctcatcgttt gttgccatta cggtcggggc gggaattacc
    35641 ctggcggtgc tgattctctc agggtcgatt gaatttatcc cgatgcaggt gatccctatc
    35701 gccgggatga ttgccggtaa cgccatggta gcggtggggt tgtgttacaa caatttaggg
    35761 caacgggtca ttagcgaaca gcaacagatt caggagaaac tgagtcttgg tgcgacgccg
    35821 aagcaggctt cagcgatatt gattcgcgac agtattcgcg cggctttaat tccgacggtc
    35881 gattcagcaa aaacggttgg cttagtgagt ttaccaggaa tgatgtccgg gctgatattt
    35941 gccgggattg atccggtgaa ggcgattaaa tatcagatta tggtgacctt tatgctgctc
    36001 tcaaccgcca gcttgtcgac cattattgcc tgctatttaa cctatcgtaa gttttataat
    36061 tcgcgccacc agttggtggt gacgcaattg aagaagaaat aatgcgatgc cggatgcgca
    36121 gcatcgcatc cggcgttgtg gttcatgtgc cgatcaacgg aatatcaata caacaatgca
    36181 tacagctggc ggcgatactt cgacgccagt gcatcacccg tacccagcgc agcgaggatc
    36241 tcctggaacg ttttacgcgt ctgaccgtct gcggcggtga gatctttacg cagatgcccg
    36301 aacagcaact ccagcgcctc ttcattgcgc ccaacctggt gcagttgcag agccagttgc
    36361 gtcgccagtg cggcatcttc tggattctcc gccacctgct gttgcaactg ttgaatttcc
    36421 ggcgtatcag ccgcctgctt cagcagttcg atttgcgcca ccagcccctg gtagcgggtg
    36481 tcctgatcct gcaacggaat ggttttcagc accgcttccg catcttcaga acggttcagc
    36541 gcaatcagcg tttctgccag cagcaggccg atctccccgt tctgattcga caactgccag
    36601 gcgtctttca gcaatggcag ggcatcggtg taattgcctt cctgcatcag ttgcatcgcc
    36661 tgctgcgctt tcagctcttc ttcgcgcggc agcactttat ccagcagggc gcggatcgcc
    36721 tcttccggtt gcggcccctg gaagccatct accggttgcc cgttctggaa cagatacacg
    36781 gtcggaatcg cgcgcagacc aaactgggcg gcaatcatct gctccgcgtc gcagtccagc
    36841 ttcgccagaa taaattgccc gttgtactgc gccgcgaggc tttccaggat cggggttaac
    36901 tgcaaacagt gctggctacg ttcagaccaa aaatagaaca gcaccggagt ggtcatcgac
    36961 tgttcaagaa cctgttgcag gttagattcg ttaatgttga caatattttc tacggacatg
    37021 gagtcgctct ctgttgtcga tattttcttt gacatggggg cttaagcgcg cgcttcaact
    37081 cacccctgca atattttgtc catcacgcgc cccggcagca ggcgcttaag caccattact
    37141 gcccaggtca ccagcgttac cggatagcgc atcttcggct tctcgctaat aaaagcatgg
    37201 cgtactttgt ccaccaccgc ttccggtccc aacgtaaagc gggcggcgat gccgggattt
    37261 tcgactggtt tatcactttg cgtctggttg acgttgtcgg tgaagcgagt acgaatggga
    37321 ccgggttcga tcaggctgac tttaattccg ctgtggcgta gctccatgcg cagcgcgtct
    37381 gaccacgcct ccagcgcata tttactggcc gcgtaagcgc cacgacccgg cgtggagatt
    37441 aatcccatca ccgatgatgt catcacaata cgcccttcac cgtgcggtaa catcgcgggt
    37501 aacaggcgca tggtgagctg gtgtgcgccg aaaaagttgg cggaaaactg ctgttccatc
    37561 tgcgcacggc tgatggtgga aagggggcca tacatgccga atccggcatt gttaaagatc
    37621 ccatacagac aattatcggt cagggcgatc acttcgtcgg ctgcgcgatc aacactttct
    37681 ggcgaatcca gatcgatcaa cacgccggta aatcccatgc tgttcatacg ctcaacatca
    37741 tccggtttcc ggcaacctgc cagcacatga aaaccctggc gttttaattc gagcgcgctt
    37801 tccaggccaa ttccactgga acatccggta attaagaccg atttttgcat aactttacct
    37861 gtcaggatct ccgttgcttt atgagtcatg atttactaaa ggctgcaact gcttcgccat
    37921 ccagtcggca ataaacggct gggcgtcgcg gttgggatga ataccgtcat cctgcatcca
    37981 ttgtggcttg aggtagacct cttccataaa aaagggcagc agcggaacat caaactcttt
    38041 ggcgagtttg gggtaaatgg cgctaaaggc ttcattataa cggcgaccat agtttgcagg
    38101 cagacgtatt tgcattaaca atggttcagc gttggcggct ttgacatcct gcaaaatctg
    38161 gcgcagcgtt tgctcggttt gctgtggctg aaaaccacgc aaaccgtcat tgccgcccag
    38221 ttcaaccagc acccaacgcg gctgatgctg tttcagcaga gccggaaggc gcgccagtcc
    38281 ttgttgcgag gtgtcgccgc tgatgctggc attaactacc gacgttttac tctgccactt
    38341 atcattcaac aaggcaggcc aggccgcgct ggcagacatt cgatacccgg cgctcaggct
    38401 atcacccaga atcaataacg tgtccgctgc ggcggcacgg aaggttaaca ggaccaggaa
    38461 caggaagggc aaatgccagc ggaaaacatt gttgaagttc atcatcttaa gaagtccgtc
    38521 ggtcaggggg agcatgaact ctccatcctc accggagttg agctggttgt caaacgtggc
    38581 gagaccatcg cactggtggg cgagtcggga tcgggtaagt caaccttgct ggcgatcctc
    38641 gccgggcttg atgacggcag cagtggcgaa gtgagtctgg tgggacaacc gctacataat
    38701 atggacgaag aagcgcgggc aaagttgcgc gcgaagcacg tcggctttgt ttttcagtca
    38761 tttatgttaa ttcctaccct taacgcgctg gaaaacgtcg agcttccggc tctgctgcgc
    38821 ggtgagagta gcgcggaaag tcgtaacggg gcgaaagcgt tgctcgaaca gttagggctg
    38881 ggtaaacgtc tggatcatct tccggcacag ctttccggcg gtgaacagca acgagtggcg
    38941 ctggcacgag cctttaatgg tcgacctgat gtgctgtttg ccgacgaacc caccggcaac
    39001 cttgaccgcc agacgggcga taaaattgcc gacctgctgt tttccctcaa ccgtgaacat
    39061 ggcaccacgt tgattatggt gacccacgac ctgcaactgg cggcacgctg cgaccgctgc
    39121 ttacggctgg tgaacgggca gttgcaggag gaagcatgat tgcacgttgg ttctggcgcg
    39181 aatggcgttc gccgtcgcta ttaattgtct ggctggcgct aagcctggcg gtggcctgcg
    39241 tgctggcgct gggcaatatc agcgatcgca tggagaaggg cttaagccag caaagccgtg
    39301 agtttatggc gggcgatcgg gcgttgcgca gttcacgcga agtgccgcaa gcgtggctgg
    39361 aggaagcgca aaagcgcggc ctgaaagtcg gcaagcagct gactttcgcc acaatgacct
    39421 ttgcaggcga cacaccgcag ctggcgaacg tcaaagcggt ggatgatatc tacccgatgt
    39481 atggcgatct gcaaactaat ccccctggcc tgaaaccgca ggcgggcagc gtattgctgg
    39541 ccccacgcct gatggcactg cttaacctga aaacgggcga caccattgac gtgggcgatg
    39601 ccaccttgcg gattgccgga gaagtgattc aggaaccgga ttccggtttt aaccccttcc
    39661 agatggctcc gcgtctgatg atgaatctgg cggatgtcga taaaaccgga gccgtgcagc
    39721 cggggagtcg ggtcacctgg cgttataaat tcggcggcaa cgagaaccag ctcgacggct
    39781 atgagaaatg gttgttacct cagcttaaac ccgaacaacg ctggtacggt ctggaacagg
    39841 acgaaggcgc gctggggcga tcgatggaac gctcgcaaca gttcctgctg ctttcggcgc
    39901 ttctgacctt gctgctggca gtggcagcgg tggcggtagc gatgaatcat tactgtcgca
    39961 gtcgctacga tctggtggcg atcctcaaaa cgctgggggc agggcgagcg caactgcgta
    40021 agctaatcgt cggtcagtgg ttgatggtgc tgacgctttc agccgttacc ggtggggcca
    40081 taggtctgtt gttcgaaaac gtgttgatgg tgctgctcaa gcccgttctg cctgctgcac
    40141 taccgccagc cagcctctgg ccgtggctgt gggcgcttgg caccatgacg gtcatctcgc
    40201 tgctggtggg gctacgacca taccgcttgt tgctggcaac gcagccttta cgcgtattac
    40261 gtaatgatgt ggtagcgaac gtctggccgc tgaagtttta tctgccgatt gtcagtgtgg
    40321 tggttgtgct gctgctcgcc ggattaatgg gtggcagcat gctgctttgg gcggtgctgg
    40381 cgggcgcggt agtactggct ttgctgtgcg gtgtgctggg ctggatgctg ctgaatgtac
    40441 ttcgccgcat gacgctgaaa tcgctgcctc tgcgcctggc ggttagccgc ctgttacgtc
    40501 agccgtggtc aacgttaagt cagctttcgg cattttcgct ctcctttatg ctgctggcgc
    40561 tgttgctggt attgcgtggc gatctgctcg accgctggca gcagcagcta ccgccagaaa
    40621 gcccgaacta ctttttaatt aacatcgcca cagaacaggt agcaccgcta aaagcgttcc
    40681 tcgcggaaca tcatatagtc ccggaatcat tttatccggt agtgcgggcg cggctgacgg
    40741 cgattaacga taagccgaca gaaggtaatg aagatgaggc gcttaatcgc gaactcaatc
    40801 ttacctggca aaatacgcgg cccgatcata atccgattgt cgccggtaac tggccgccaa
    40861 aagccgatga agtgtcgatg gaagaggggc tggcgaaacg cttaaacgtt gccctcggtg
    40921 ataccgtgac ttttatgggc gatacccagg agttccgcgc taaagtgacc agcctgcgca
    40981 aagtggactg ggaaagtctg cggcctaatt tctattttat tttcccggaa ggggcattag
    41041 acgggcaacc gcagagctgg cttaccagtt tccgctggga gaatggcaac ggcatgttga
    41101 cgcaactcaa ccgccagttc ccgaccatta gcctgttaga tattggcgcg attttaaaac
    41161 aggtcggtca ggtgctggag caggtaagtc gggcgctgga ggtgatggtg gtgctggtca
    41221 ccgcctgcgg tatgttgctg ttgctggcac aggtgcaggt gggaatgcgc cagcgtcatc
    41281 aggagctggt ggtatggcgc acactcggtg cggggaaaaa actgctgcgc acgacattgt
    41341 ggtgtgagtt cgccatgctt gggtttgttt ccggcctggt ggccgcaatt ggtgcggaaa
    41401 cggcactggc agtgttgcag gcgaaagtgt ttgattttcc gtgggagcca gactggcgat
    41461 tgtggattgt tctgccgtgc agcggtgcgc tgctgctgtc gcttttcggc ggctggctgg
    41521 gtgcgcgact ggttaagggt aaggcgctgt tcaggcagtt tgcggggtga tgacaggcgt
    41581 tagattgcct gatgcgctac gcttatcagg cctacgaaga gcgtgcaata tattgatttt
    41641 tcaagattgt gtaggccgga taaggcgttt acgccgcatc tggcatgaac agtgtgcatt
    41701 ttggcaataa atatgaaacc aactgtcatg tcggtttttt taaaggccga aggtggtaaa
    41761 ccttcggcct tagctacgtt tcctcagtta ttcaataaac tgacggatca gttccagcag
    41821 cgcgattagc gccttaagaa cgatgagaac tttatcgaga aaagttttca tccgttgctc
    41881 ccttaaggga gaaagacaat cacagcttac ccttacgttg cctgtaacta tgattttctt
    41941 catgtgctag atgaggactg cctctctcca aattagagca ccagaatcag caccacctgt
    42001 atcctgacca ggacttaaaa gtacttttcc gcactgcggt aatcccttga ggcagtatca
    42061 ctactagcgt tgggattgta tcgccaacat agctgtttga aatgtaggca tcctgactta
    42121 taattcactt gctagatgag gactgtttca tttctgcccg gaaccaccaa gtcttgggca
    42181 gaaaaaataa aaaaaccgac tgtcatgtcg gtttttttat atctgtattc tggtgcaacc
    42241 attctgttct cacgacaatc gccgtttcag aataccgccc atcactgcgc cttaacccat
    42301 tccgccactt ccgcccactc accgcgaaag acaacttttt ccgctttttt ctcaagctga
    42361 tagcgataca tcgggtcgta atattcttca agtaacggca ccagccaggc cagatgaccg
    42421 tcggtgctgc cggtggtgag ttgcgttgtc agtgctgcat ccagccttgc agccagttcg
    42481 ttatatcgct gtagccccag ccgacgctta atcgccgaaa gtccgtgatg caggtattcg
    42541 caatattcct gccagccctg ttcgtcgcca taagcgtggg taaaatcatg atgcatacgc
    42601 aagaaatact cttcgttcag gcgttcaaga cggatctcaa acggatcttc taccaccgca
    42661 atcgccgcct gagtcattcg ctcgcgcaga cattccggca ggtgattcga accgatcatc
    42721 cggctttcgt cttccagcac ccacaggcgc aaattctgac gggcgtcggt ttttagcatt
    42781 tcggcagcca gcaggttttc aaaactcgcc tggctaagtt gtggttgtaa ggtgcgacca
    42841 aacgccgaac cgcgatgacg cgccaaccct tccagatcaa caccgttcgg ctgttgctgc
    42901 actaacagcg ttttgccgca gccggtacaa ccgccaatca gcactatcgg tttttgtgac
    42961 agttcaatgg tcgcctgaat cgcagtctgg cgcagtacct tataaccgcc ttccaccagc
    43021 ggataattaa tccccacttc atgcaaccag cgttgcacaa tatgtgaacg ttgaccgcca
    43081 cgggcgcagc agagaatacc ttgcggattt tgcaggcacg ctgcccgcca ggcgtccatg
    43141 cgctgctgac gaatttcacc cgccaccagt ttatgtccca gcgccagcgc tgcgtctgag
    43201 tcttgctgtt tatagcaggt gccaacggcg gcgcgttcat cgttattcat taacggcaga
    43261 ttgatagcgg cgggcattgc gccgtgctca aactcgatag gggcgcgaac atcaataatg
    43321 ggcgtatcag caatcagcag ggcacgatag tcctgttccg tgtgtctctc ttgcatagtt
    43381 aaaagtgaac ctcaaatcag cttgcgcgct attttacgcg ccaacgcgca aggaaacttg
    43441 atttttaact gcgtgggttg ccgaaaattt ctaaaaatcc gctgatttcc ggcctgcgtt
    43501 gggtaaacaa ggtcacaata tcttctaccg ctttgccgct gccaaatttg cgccatgcca
    43561 gactcaatgg cgaaggaggg cgcatcgttg ggattacccg gctgaccagt tgttgattat
    43621 cgatcattga ctggcaaagt gattttggca aaaaaccaat gccaacaccc gccagatggg
    43681 cggcgatttt cgtttccata tcaggaacaa taatctcttt ttgccctggc aatcgccagg
    43741 cgacgcgttt ggttaaggtg cgggcgctgt cttcaatatt gaccgccgga aagcgccgca
    43801 actgcgcttc tgttagcggc tcttcaacgt tcgccagcgg atgatccgcc gccatgacaa
    43861 agcgccattg caccgatcct aagggatcaa gactaaaggt atttgccagc gcctcggttc
    43921 ccgtgacgcc gatagccagt gaaaaacctt cgtacaatag cgagtcccag acgcccatat
    43981 agatttgtcg ggagatgtga aactgggtaa aggggtaacg ttcattcagc cacgccagca
    44041 actgggcgac ggcctggggg ttgtagagca ggttgttgat gacaatattc acctggcgtt
    44101 ccacgccatc attcacctgt tgcagctcgc ttggcatact ttccagccag ctcagccagt
    44161 ctctggcctg ggaaagtaga tgctcgccag ccgctgtcaa cgtcacgctg cgagtcgtgc
    44221 ggaaaaacag cgctactccg gtattctctt ccagaagttt aatgcgataa ctgatcgtcg
    44281 ctgtggtttt acataatcgt tctgccgctt ttgaaaaact tcctgtttca gcaaccgcaa
    44341 tgaaagtccg caaggtttct ggatcgaaca tcttcaggta tcccctttta aatccgcaag
    44401 ttgcgtgatt ttcttatcct ctgatttatc agtattttta catgataacc ctgttcaatt
    44461 tgtggactaa atctagtttt ggaaaaatat tccaactttt gtattgaagt tgttctctta
    44521 aggttttaga ttgcctgtta ttgaaaccaa gctgaccggt cggcggtggt tgaacggaat
    44581 tatgttacaa ggacaaaaag atgaaacttc aggtattacc gttaagtcag gaagccttta
    44641 gtgcttatgg cgacgtaatc gaaacgcagc aacgggattt tttccatatt aacaatggcc
    44701 tggtggagcg ttaccacgat ttggcgctgg ttgagattct tgagcaagac cgtacgctta
    44761 tcagcattaa ccgcgcgcaa ccggcgaatc taccgctgac cattcacgaa ctcgaacgtc
    44821 atccgctggg tactcaggcc tttatcccga tgaaaggtga agtgtttgtg gtggtcgtgg
    44881 cgttaggtga cgacaaacca gacctgtcaa ccctgcgggc gtttatcacc aacggtgaac
    44941 agggggtgaa ttaccatcgt aacgtctggc atcacccact tttcgcctgg cagcgcgtca
    45001 ccgattttct gaccatcgat cgcggcggca gtgacaactg tgatgttgaa agtattcctg
    45061 aacaggaact ctgttttgcg tgacgcctgc aaccgacttg cataagataa attaattgtt
    45121 cattgtttat gctcacttgt aggtcggagt taacgtaggt atgacggaag ttagacggcg
    45181 cggcaggcca ggacaggcgg agcctgtggc acagaagggc gcacaggcgt tagagcgggg
    45241 aattgcgatt ctgcaatatc tggaaaaaag tgggggaagt tcgtcggtta gcgatatttc
    45301 tctcaatctg gatttgccgc tctccacgac ctttcgcttg ctgaaggttt tacaggcagc
    45361 ggattttgtc tatcaggaca gtcagttagg ctggtggcat ataggattag gtgtctttaa
    45421 cgtcggtgcg gcgtacatcc ataaccgcga tgtcctctcc gtcgccgggc cgtttatgcg
    45481 ccgcctgatg ttactttccg gcgaaacggt caatgtcgcg atccgtaacg gcaatgaagc
    45541 agtattaatt ggtcagctag agtgtaaatc gatggtcagg atgtgtgcgc cactgggcag
    45601 tcgtctgcca ctacatgctt ccggtgcggg caaagcgctg ctttatccgc tggcggaaga
    45661 ggagttgatg agcatcattc tgcaaaccgg tttgcagcag tttacgccaa ctacgcttgt
    45721 ggatatgccc accttactga aggacctgga gcaagcgcgt gaactgggct ataccgtaga
    45781 taaagaagag catgttgtag gtctgaattg catagcttca gcaatttacg atgatgtagg
    45841 tagtgttgtt gccgctatct ccatctccgg gccttcatca agactgacag aagatcgttt
    45901 tgtcagtcag ggtgagctgg tcagagacac cgcccgcgat atcagcacgg cgttgggact
    45961 gaaagcacat ccatagtgtc tgtcgcatcc cgctctgcgg agcgggtttt tttgacaaaa
    46021 tttgaaagtt agaaaatttt tccaattaat agaggtagga ataaaatggc aaaaatgaga
    46081 gccgttgacg cggcaatgta tgtgctggag aaagaaggta tcaccaccgc cttcggcgtt
    46141 ccgggagctg caatcaatcc gttctactca gcgatgcgta agcacggcgg tattcgtcac
    46201 attctggcgc gtcatgtgga aggtgcttcg cacatggcgg aaggttatac ccgcgcaacg
    46261 gcagggaata tcggcgtatg tctggggact tccggtcctg cgggcacgga catgatcacc
    46321 gcgctctatt ccgcttctgc tgattccatt cctattctgt gcattaccgg ccaggcaccg
    46381 cgcgcccgtc tgcataaaga agattttcag gccgtagata ttgaagcaat tgctaaaccg
    46441 gtcagcaaaa tggcggttac agttcgtgaa gcggcgctgg tgcctcgcgt gctgcaacag
    46501 gcatttcacc tgatgcgttc tggtcgtccg ggtccggtac tggtggattt accgttcgac
    46561 gttcaggttg cggaaatcga gtttgatcct gacatgtacg aaccgctgcc ggtctacaaa
    46621 cctgctgcca gccgtatgca gatcgaaaaa gctgtagaaa tgttaatcca ggccgaacgt
    46681 ccggtgattg ttgccggggg cggggtaatt aatgctgacg cagctgcact gttacaacag
    46741 tttgctgaac tgaccagcgt tccggtgatc ccaacgctaa tgggctgggg ctgtatcccg
    46801 gacgatcatg aactgatggc cgggatggtg ggtctgcaaa ccgcgcatcg ttacggtaac
    46861 gcaacgctgc tggcgtctga catggtgttt ggtatcggta accgttttgc taaccgtcat
    46921 accggctcgg tagagaaata caccgaaggg cgcaaaatcg ttcatattga tattgagccg
    46981 acgcaaattg gtcgcgtgct gtgtccggat ctcggtattg tctctgatgc taaagcggcg
    47041 ctgacactgc tggttgaagt ggcgcaggag atgcaaaaag cgggtcgtct gccgtgtcgt
    47101 aaagaatggg tcgccgactg ccagcagcgt aaacgcactt tgctgcgcaa aacccacttc
    47161 gacaacgtgc cggtgaaacc gcagcgcgtg tatgaagaga tgaacaaagc ctttggtcgc
    47221 gatgtttgtt atgtcaccac cattggtctg tcacaaatcg ctgcggcaca aatgctgcat
    47281 gtctttaaag accgccactg gatcaactgt ggtcaggctg gtccgttagg ctggacgatt
    47341 ccggctgcgc taggggtttg tgccgctgat ccgaaacgca atgtggtggc gatttctggc
    47401 gactttgact tccagttcct gattgaagag ttagctgttg gcgcgcagtt caacattccg
    47461 tacatccatg tgctggtcaa caacgcttat ctggggctga ttcgccagtc gcagcgcgcg
    47521 tttgatatgg actactgcgt gcaactcgct ttcgagaata tcaactccag cgaagtgaac
    47581 ggttacggcg tcgaccacgt aaaagtagcg gaaggtttag gttgtaaagc gattcgcgtc
    47641 ttcaaaccgg aagatattgc gccagccttt gaacaggcga aagccttaat ggcgcaatat
    47701 cgggtaccgg tagtcgtgga agttattctc gagcgtgtga ccaatatttc gatgggcagc
    47761 gaactggata acgtcatgga atttgaagat atcgccgata acgcagcgga cgcaccgact
    47821 gaaacctgct tcatgcacta tgaataaggg agataaataa tgttacgttt ctctgctaat
    47881 ttatcgatgt tatttggaga atatgatttt ctcgcccgtt ttgagaaagc tgcgcagtgt
    47941 ggttttcgcg gcgttgaatt tatgtttcct tatgactacg acattgaaga attaaaacag
    48001 gtgctggcga gtaataaact cgaacatacg ctgcacaatc taccggcggg tgactgggcg
    48061 gcgggggagc gcggtattgc ctgtattcct ggccgtgaag aagagtttcg ggatggcgta
    48121 gcagcagcga ttcgttatgc ccgtgcgctg gggaataaaa aaattaactg tctggtcggt
    48181 aaaacgccgg ctggtttcag cagtgaacag attcacgcaa cgcttgtaga aaacctgcgt
    48241 tatgccgcga atatgctgat gaaagaagat attttattac tgattgaacc tattaaccat
    48301 tttgatattc ctggtttcca tctcaccgga actcggcagg cgctgaaatt gattgatgat
    48361 gttggttgct gcaatttgaa aattcagtat gacatttatc acatgcagcg gatggaaggt
    48421 gaattaacca acaccatgac tcagtgggcc gataaaattg gccacctgca aattgctgat
    48481 aatccgcatc gcggcgaacc ggggaccgga gaaattaatt atgattatct ctttaaggta
    48541 atcgaaaatt ctgactacaa cggttgggtt gggtgtgaat ataaacccca aaccaccacg
    48601 gaagccggtt tacgctggat ggatccgtac cgttaaaacg taacgctgtt caggcaatgc
    48661 ttttttaggc cgctaagttg gcaggggatc gtgttgtctg aattcaggaa aagcgaaatt
    48721 taaaagaggt taattatgaa actgggattt attggcttag gcattatggg tacaccgatg
    48781 gccattaatc tggcgcgtgc cggtcatcaa ctgcatgtca caaccattgg tccggttgct
    48841 gatgaattac tgtcactggg tgccgtcagt gttgaaactg cgcgccaggt aacggaagca
    48901 tcggacatca tttttattat ggtgccagat acaccacagg ttgaagaagt tctgttcggt
    48961 gaaaatggtt gtaccaaagc ctcgctgaag ggcaaaacca ttgttgatat gagctccatt
    49021 tccccgattg aaactaagcg tttcgctcgt caggtgaatg aactgggcgg cgattatctc
    49081 gatgcgccag tctccggcgg tgaaatcggt gcgcgtgaag ggacgttgtc gattatggtt
    49141 ggcggtgatg aagcggtatt tgaacgtgtt aaaccgcttt ttgaactgct cggtaaaaat
    49201 atcaccctcg tgggcggtaa cggcgacggt caaacctgca aagtggcaaa tcagattatc
    49261 gtggcgctca atattgaagc ggtttctgaa gccctgctat ttgcttcaaa agccggtgcg
    49321 gacccggtac gtgtgcgcca ggcgctgatg ggcggctttg cttcctcacg tattctggaa
    49381 gttcacggcg agcgtatgat taaacgcacc tttaatccgg gcttcaaaat cgctctgcac
    49441 cagaaagatc tcaacctggc actgcaaagt gcgaaagcac ttgcgctgaa cctgccaaac
    49501 actgcgacct gccaggagtt atttaatacc tgtgcggcaa acggtggcag ccagttggat
    49561 cactctgcgt tagtgcaggc gctggaatta atggctaacc ataaactggc ctgatacccg
    49621 caataaaaat ggccgatatc agaaaatgaa tcggccagca atattaaaaa agaaagcagc
    49681 caaagatgtt gcttcagtat taaaaataat atttttattt tatttgttcc tcatagctag
    49741 attaaaacaa cgttattcga tacgtgaaat taagagggat ttatggaaca tcagagaaaa
    49801 ctattccagc aacgcggcta tagcgaagat ctattgccga aaacgcaaag ccagcggacc
    49861 tggaaaacat ttaactattt taccttatgg atgggttcgg ttcataacgt tcccaattat
    49921 gtgatggtcg gcggcttttt tattctcggc ttgtctacct ttagtattat gctggcaatt
    49981 atcctcagcg cctttttcat tgccgcggta atggtattaa acggcgctgc gggcagtaaa
    50041 tacggcgtac cgtttgccat gatcctgcgt gcttcttacg gcgtacgtgg cgcactgttt
    50101 cccggattat taagaggcgg tattgctgcc attatgtggt ttggcctgca atgttacgcg
    50161 gggtcactgg cctgcttgat tctgattggc aaaatctggc cggcattttt aactctcggt
    50221 ggtgatttca cgctgttagg gctttctcta ccgggcttaa ttactttctt actcttctgg
    50281 ctggtcaacg ttggtatagg ttttggcggt ggcaaagttt taaataaatt cactgccatt
    50341 cttaacccgt gcatctatat cgttttcggc ggtatggcga tttgggcgat ttcgctggtc
    50401 gggctcggtc caatctttga ctacattccg agcggtattc agaaagcaga aaacagtggc
    50461 ttcctgttcc tggtggtgat aaacgcggta gttgcggtct gggcggcacc ggcggtgagc
    50521 gcatccgact ttacgcaaaa cgcccactcg tttcgtgagc aagcgctggg gcaaacgctg
    50581 ggtttagttg tggcctatat tctgtttgcg gtcgcggggg tatgtattat tgccggagcc
    50641 agtattcatt acggcgctga tacctggaac gtgctggata ttgttcagcg ttgggacagc
    50701 ttgttcgcct cgttctttgc ggtactggtt attctgatga cgactatctc cactaacgcc
    50761 accggtaata ttattcctgc cggttatcag attgccgcta ttgcaccgac gaaactgact
    50821 tataaaaacg gcgtactgat tgccagtatt atcagcctgc tgatctgccc gtggaaatta
    50881 atggaaaatc aggacagtat ttatctgttc ctcgatatta tcggcggaat gcttggtccg
    50941 gtaattggtg tcatgatggc gcattatttt gtggtgatgc gtgggaaaat taatcttgat
    51001 gaactgtata ccgcacctgg cgattataaa tattacgata acggttttaa cctcactgcg
    51061 ttttcagtaa ctctggtggc cgttatttta tctcttggcg gtaagtttat tccctttatg
    51121 gaaccgttat cgcgtgtttc atggtttgtc ggcgtcatcg tcgcctttgc ggcctacgcc
    51181 ttattaaaga aacgtacaac agcagaaaaa acaggagagc aaaaaaccat aggttaatta
    51241 atcccgatat tgaacattga gttaaaaacc aatctgtatt ttacaaggag tttgttatgt
    51301 cttttgattt aatcattaaa aacggcaccg ttattttaga aaacgaagct cgcgttgtag
    51361 atatcgccgt taaaggcgga aaaattgctg ctatcggtca ggatctgggc gatgcaaaag
    51421 aagttatgga tgcgtctggt ctggtggttt cgccgggcat ggttgatgcg cacacccata
    51481 tttctgaacc gggtcgtagc cactgggaag gttatgaaac cggtactcgc gcagcggcaa
    51541 aaggtggtat caccaccatg atcgaaatgc cgctcaacca gctgcctgca acggttgacc
    51601 gtgcttcaat tgaactgaag ttcgatgccg ctaaaggcaa gctgactatc gatgcggcac
    51661 aactcggtgg cctggtgtct tacaacattg atcgtctgca tgagttggat gaagtgggcg
    51721 ttgtcggctt caaatgcttc gttgcgacct gtggcgatcg cggtatcgac aacgacttcc
    51781 gtgatgtaaa cgactggcag ttcttcaaag gtgcgcagaa gctgggcgaa ctggggcagc
    51841 cggtgttggt gcactgcgaa aacgcgctga tctgtgacgc actgggcgaa gaagcgaaac
    51901 gtgaaggtcg cgtaactgcc catgactatg tggcttcgcg tccggtattt accgaagtgg
    51961 aagcgattcg ccgcgtgctg tatctggcga aagttgccgg ttgccgtctg cacgtttgcc
    52021 atatcagcag cccggaaggt gttgaagaag tgactcgtgc acgtcaggaa ggtcaggatg
    52081 ttacctgtga atcctgcccg cattactttg tactggatac cgatcagttc gaagaaatcg
    52141 gcactctggc gaagtgttca ccgccgatcc gcgatctgga aaaccagaaa ggcatgtggg
    52201 aaaaactgtt taacggtgaa atcgactgcc tggtttccga ccactcacca tgccctccgg
    52261 aaatgaaagc cggcaacatc atggaagcat ggggcggtat tgccggtctg caaaactgta
    52321 tggacgtgat gttcgatgaa gcggtacaga aacgcggaat gtctctgcca atgttcggca
    52381 aattaatggc gactaacgca gcagatattt tcggtctgca gcaaaaaggc cgtatcgccc
    52441 caggaaaaga tgccgacttc gtcttcattc agccgaatag cagctatgtt cttaccaatg
    52501 acgatctgga atatcgccac aaagtcagcc cgtatgttgg ccgtaccatt ggcgcgcgta
    52561 tcacgaaaac catcttacgt ggtgatgtga tttacgacat tgaacagggc ttccctgttg
    52621 cgccgaaagg tcaatttatc cttaaacatc agcagtaatc tggcccctgc aatgcccgtc
    52681 cttgcggcgg gcattctccg gttaaggtgt gtttatgttc aattttgcag tcagccgcga
    52741 aagcctgtta tcaggatttc agtggttttt ctttattttt tgcaacacgg ttgtggttcc
    52801 tcctacgcta ctttctgctt ttcagttgcc gcaaagtagc ctgcttacgc tcacgcaata
    52861 tgcttttctt gctaccgcac tggcctgctt cgctcaggcg ttctgcggtc atcgtcgcgc
    52921 tattatggaa gggccgggtg gcctgtggtg gggaaccatc cttactatca cccttggcga
    52981 agcatcgcgc gggacaccga tcaacgatat cgctaccagc ctggcggtgg ggattgcgct
    53041 ctccggcgtg ctgacgatgt tgattggttt tagcggatta ggccatcgcc tggcacggtt
    53101 atttacgccg tcggtgatgg tcttgtttat gttgatgctg ggcgcgcagc tgaccactat
    53161 ctttttcaaa ggtatgctcg gactgccgtt tggcatagcc gacccaaatt ttaaaattca
    53221 gttaccaccg ttcgcgctct cggtggcggt gatgtgcctg gtactgggga tgattatctt
    53281 cctgccgcaa cgttttgccc gttatggcct gctagtcggc accataaccg gctggttgtt
    53341 gtggtatttt tgctttcctt cttcgcactc gctctccggt gagttgcact ggcagtggtt
    53401 cccgctcggc agtggcggtg ctttgtcgcc ggggattatt ctgacggcgg tgattacagg
    53461 tctggtaaat atcagcaata cctacggtgc gattcggggc acggatgttt tttatccgca
    53521 gcagggcgcg gggaatacgc gttatcgtcg tagctttgtg gcgaccggat ttatgacact
    53581 gataaccgta ccgctggcag taattccatt ttcaccgttt gtttcatcca ttggtttatt
    53641 aacccagact ggcgattaca cgcggcgttc gtttatttat ggcagcgtta tttgcctgct
    53701 ggtggcgctg gttccggcac tcacgcgact gttttgcagt atccctttac ccgtgagtag
    53761 cgcggtcatg ctggtttctt atctgccttt actcttttcc gcgctggtgt ttagccagca
    53821 aataacgttt accgctcgca atatttatcg actcgcattg ccgttatttg tcggcatatt
    53881 tttaatggca ttaccgcctg tgtatctgca agaccttcca ttaacgcttc gtcctctgct
    53941 cagtaacggc ttattggtcg ggattttact ggctgttctt atggataacc ttattccgtg
    54001 ggaacgtatc gaataatttg ttgaaaaagg attgataatg aagattgtca ttgcgccaga
    54061 ctcttttaaa gagagcttaa gtgcagaaaa atgttgtcag gcaattaaag ccgggttttc
    54121 gaccctcttt cccgatgcga actatatctg tttgccgata gcggatggcg gcgaaggaac
    54181 ggtggatgcg atggtcgccg cgacgggcgg caacatcgtg acgcttgaag tctgcgggcc
    54241 gatgggcgaa aaagtgaatg ctttttatgg ccttaccggc gacgggaaaa cggcggtgat
    54301 tgagatggcg gcagcaagtg gcctgatgct ggtcgcgcct gaaaagcgta atccgttgct
    54361 ggcctccagt tttggtacgg gggagttaat tcgtcatgcg ctggataacg gcattcgcca
    54421 tattattctc ggcattggcg gcagtgcgac ggtcgacggc ggtatgggca tggcgcaagc
    54481 gcttggtgtg cgttttctcg atgccgacgg tcaggtgctg gcagctaacg gcggcaattt
    54541 agcgcgcgtg gcaagcattg agatggagga atgcgatccg cgtctggcga acagccatat
    54601 tgaagtagca tgtgacgttg ataacccgct ggtaggggca cgcggcgcgg cggcggtgtt
    54661 tggcccgcaa aaaggggcaa cgccggagat ggtcgaagag ctggaacagg ggctgcaaaa
    54721 ttacgcccgt gttttacaac agcaaactga aattaatgtc tgtcagatgg cgggcggcgg
    54781 cgctgcgggc ggtatgggta ttgcggcggc ggtatttctc aatgcggata ttaaaccggg
    54841 cattgaaatt gtgttgaatg cagtcaatct tgcgcaggca gtgcagggcg cagcactggt
    54901 gatcaccggg gaagggcgca tcgactcgca aacggcaggc ggtaaagcgc cgctgggtgt
    54961 ggcgtcggtg gcgaaacagt ttaatgtacc ggtgattgga attgctggcg tattgggtga
    55021 tggcgtggaa gtggtacacc agtacggcat tgacgcggta ttcagcattt tgccacgtct
    55081 ggcaccttta gccgaagtgc tcgccagcgg tgaaaccaat ctcttcaaca gcgcgcgaaa
    55141 tattgcctgc gccattaaaa taggtcaggg aattaaaaac tgactcttgc ctttaaagcg
    55201 gatgctattt atttcgtata agagcgcagt actcatgccg gatgcggcat gagtactata
    55261 tccttcctga aaatcgcgca aattctatat attgcagaga tcatgtaggc ctgataagcg
    55321 aagcgcatca ggcaatgtta caaaaaaagc cacggtataa accgtggcaa aatccaacat
    55381 agctaaaaat aatcaggcga gtggtatgac ttaaatctct acgtcgcggt tgcaatcttt
    55441 cgagtaaata tagctgaacg cttcaccacg ccctacgcca taaccagcct gtaaagaata
    55501 agcgcccata aagatgtaat cgcctttttt caccgggatc cagttattgt cgaggttata
    55561 aaccccctga ccggaaagaa tataggcacc atgttcctga acgtgtgttt cgatataacc
    55621 gtggctggca cctggcgcaa aagagaggat atgcatgttc atatcaaaac ctaactcttt
    55681 gggcaggaaa tccagcagga tcacatcatc cataccttca tagtgaatgc gttccagttc
    55741 gctggcatta ccagaaacca gccacggtgc atggccttct accggaacat agcggcgctt
    55801 atataaaaag atttggctgt cttcggcctg ggcgttaaca aacgtcatta aggagcctgg
    55861 tgggcaataa agatagccac cttcgcttaa ggcaaatgtt ttgccttcgg ctttggcagt
    55921 gatatttcca gagatcacat acaggaacgt ttcaatgcct tcgccaccga agccctgttg
    55981 gttgccaccg ttttgatgca gtgtgaccag ataatcaaca aaagaggcac ccagctttgg
    56041 tgtggagagg attgtcgcgt cacaattttc aaagcccgga ataatatttt ttaccagacc
    56101 gtctggtgtt aacagtgcga aattaccgtg tttcacaatc gcacggttag ccagtaaatc
    56161 atcgcggtaa ccggtgacgt tatttaaata tcccatttat aactccttat ttctgccagg
    56221 caagttgata aagcatgagt gccaacgttt tgaccccttc ggcaaggtcg gtaatattgg
    56281 tgcgttccgc cgggttatgg ctgatcccgt tgatgctggg gataaaaatc atgcaggttg
    56341 gtacgcgagg cgcgaaaatt tgcgcgtcgt gcccggcacc actgtgcatc acccggtaat
    56401 tcagtttttc gctttcacac aattctgtca gggtggcgac cagctcctta ttcatcggca
    56461 cgggttcttc gtccatccat aaatcgatat caataccaat gtccatttca tcgcaaatcg
    56521 cccgcatgtc gttttctaac tgttgggtga aatcgcgcag cacggcagcg tcggtatgac
    56581 gacaatcaat ggtgaacgtg gttttacccg gcaccacatt taccgtattc gggcgcggct
    56641 ctactttgcc aaaggtcaga accagcggat cgcccatcct tttcgctttt tcgaccgact
    56701 gatggcaaat gcgactgaaa gcgtaaactg tatcacgacg ataacccatc ggcgtggtgc
    56761 ctgcatggtt tgattcgccg ttcagcgtta ccgtataacg acgctgcccg acaattgcat
    56821 tcaccacgcc aattgattgc ccattacttt ccagcacaca gccctgttca atatgcagtt
    56881 caacaaaggc tttaatatcc tgacgcggag ttagtggggc gttcggaaga gtaaatccgc
    56941 aagccttcat cgcatcgaca aaactatttc ctttggcatc acagatattc cgcacgtcgt
    57001 caggattcgc cagcccaaag atatttttac tgccccagaa gacatacggg aagcggctgc
    57061 cttcttcttc tgccatcgcc accacttcga ccgtacgtag cggcgcgccg tattgcgttt
    57121 tcagccagtc aattgccagc cacgccgcca gcgcgccgaa ttgcccgtcg aggttaccgc
    57181 cgttaaccac ggtatcgata tgcgaaccgc tcagaaccac ttcttgtgga tattcggtgc
    57241 cactcaggcg accatataaa ttccccactt catcgaaacg tgtttccagc ccgcttgctg
    57301 ccattctttt tttaaattgc tgctgagttt ccagccattc cggcgaataa agtaaacggg
    57361 tcataccacc cgttgggtca gcgccaaaag aggaaagcca gggcagcgtt tcttctatgg
    57421 cttgacgaaa atgtgtaatc ataagaaagt cctgtctcaa taattattgc gcaaagggat
    57481 ttttcgtttc gtatgacgtg ttataaagcg cgtcggaaat taaatactgg taaatatcat
    57541 caacaatttc gatgccttcg acggcggctt tgcgttgttt aatatcctga tcctgtccgg
    57601 gataataaac ctgattaaaa ccgggcgcgg gggtaatggc atttaattcg cgcatggtct
    57661 ggctaagatg ttgacggaat aattcgctgg aggagaaaaa gttcgggtta ataactacat
    57721 gtaattgccc caaattacgc ccggcgtgta aatcgtcata catcgaacta acctgtcgcc
    57781 cgaacggtaa gccgagtaag acgcctgaga ggacgtcaat catcatcatc aggccatacc
    57841 ctttcggccc ggcggcgggg agcagagcat gtaccgcgaa cggatcggtt gttggtgcac
    57901 cgtttttatc gaccgcccaa gtatccggga tagacatatt acgcgagcgg gcgtcgagca
    57961 cttttcccca tgcctgtacg gtagtcgcca tatcaaaggt aaggatctcg ttgccttctc
    58021 ccggcgcggc aaaggccagt gggttagtac cgtagtaaat ttccgcgccg ccaaacggca
    58081 ccaccattgg atcggactgg cacatcgaaa tgccaattaa tccggcgcgg gctgcctgct
    58141 gcacaaaata agagattgcg ccgctgtgac ccatccggct gataccgacc accgcaacgc
    58201 cattttgctg ggcggttttg atggcatgtt ccatacccat tttcgccgcg acctgtccgg
    58261 cggcattgtc ggcatgtaaa attgccgagc acggcccggt ttcctcaaga cgaaactccg
    58321 gttcgcggtt ggtaccgcct tttgaaatgc gttccgcgta gtattccacg cgcaccgcgc
    58381 catgagagtg gatccctctg gcatcggcgt aaaccaatac ttcagccacg gttgcagcgt
    58441 gctcacgttt taacccagcc tggcagagtt tattctcaat tagctggtgg agtgtttccc
    58501 gactgatttt catctgtctt cctttttaac gacggtgtga agcatgactg caattaacat
    58561 acagggaaaa tatctggatt atgtgatcca gacaggcaaa aaaatatagt tagaatttat
    58621 ttgataatcc gctcgctttt aacctggttt ttaaaacaac cacgcttatt aaaaaataac
    58681 acgtaatagc atggcggata tttgaattct tttgttaata atccctgtgt gatattcatc
    58741 accttattta cccgttgtca tcgataccgt aatcgccaca ttaacactgc tcgtgcaatt
    58801 gccatgggtg caatttttaa ggagttgtta tgatccacgc ctttattaaa aaagggtgtt
    58861 ttcaggattc ggtcagttta atgattattt cacgaaaact cagcgaatca gaaaatgttg
    58921 atgatgtttc cgtaatgatg ggtacgcccg ccaataaagc gttattagat accacaggtt
    58981 tctggcatga cgattttaat aacgccacgc cgaacgatat ttgcgtggca attcgtagcg
    59041 aagcggcgga tgcggggatc gcgcaggcga ttatgcagca gcttgaagag gcgctaaaac
    59101 aactggcgca gggggcaggc agcagccagg cgttgacgca ggtgcgtcgc tgggacagtg
    59161 cctgtcagaa attacccgac gccagtctgg cgctgatttc agtggctggt gagtatgcgg
    59221 cggagctggc gaaccaggcg ctggatcgca acctcaacgt gatgatgttc tccgataacg
    59281 tcacgctgga agatgaaatc caacttaaaa cccgcgcgcg ggaaaaaggc ttgctggtga
    59341 tggggccgga ctgcggtaca tcgatgattg ccggcacgcc gctggctttt gctaacgtga
    59401 tgccggaagg caacattggc gtcattggcg cttccggtac cgggattcag gagctgtgct
    59461 cgcagattgc gctggcaggg gagggaatta ctcacgcgat tggccttggc gggcgcgacc
    59521 tcagccgtga agtaggcggc atcagtgcgc tgacggcgct ggaaatgctc agtgcagacg
    59581 agaaaagcga agtgctggca tttgtttcaa aaccgcctgc cgaagctgtg cgtctgaaaa
    59641 ttgttaatgc catgaaagca accggcaaac cgacggtggc gctgttttta ggttataccc
    59701 cggcggtggc ccgcgacgag aatgtctggt ttgcctcctc gctggatgag gccgcacgcc
    59761 tggcttgcct gctttcacgt gtcacggcgc gacgtaacgc aatagcgcct gtcagcagcg
    59821 gatttatttg cggtttgtat accggcggta cgctggctgc cgaagcggcg ggattacttg
    59881 ctggacacct tggcgtggaa gccgacgata cccatcatca cggcatgatg ctcgacgccg
    59941 atggtcacca gattattgac ctcggcgatg atttctacac cgtcgggcgt ccccatccga
    60001 tgatcgaccc agccttacgc aaccagttaa ttgccgatct cggcgctaaa ccgcaagtgc
    60061 gcgtattgct gcttgatgtc gtgattggct tcggtgcgac cgccgatcct gccgcctcgc
    60121 tggtaagcgc ctggcaaaaa gcatgtgccg cgcgttcaga caatcaacca ctgtatgcca
    60181 ttgccacggt gacaggcact gaacgtgacc cgcaatgccg ctcgcagcaa atcgccacgc
    60241 tggaagatgc agggatcgcg gtagtgagtt cgctgccgga agccaccttg ctggcggcgg
    60301 cactgattcg tccgctttcg cctgccactc agcaacacac accgtcatta ctggaaaacg
    60361 tcgccgtgat taacatcgga ttacgcagct ttgcgctgga gctacaaagc gccagcaaac
    60421 cggttgtgca ttaccaatgg tcgccagtcg ccggcggcaa taaaaaactg gctcgtttat
    60481 tagaacgttt gcaataaggg gttcccatgt ttacatcagt agcgcaagcc aatgctgcgg
    60541 ttatcgaaca aattcgtcgc gctcgtccac actggctgga tgtgcaaccg gcttcttcac
    60601 ttatcagcga actaaacgag ggcaaaacac tgcttcacgc cgggccgcca atgcgctggc
    60661 aggagatgac cggacccatg aaaggggcgt gcgtgggcgc atgtctgttc gaaggctggg
    60721 tgaaagatga agcgcaggcg ctggcaatac tggagcaggg ggaggtgaac ttcattcctt
    60781 gtcaccatgt gaatgccgtc ggtccaatgg gcggtattac ttctgccagt atgccgatgc
    60841 tggtggtcga gaacgtgacc gacggcaacc gggcgtactg caacctcaac gaaggtatcg
    60901 gcaaagtgat gcgttttggc gcttacggcg aagatgtcct gactcgccat cgctggatgc
    60961 gcgatgtgtt aatgccagta ttaagcgcgg cgctggggcg catggagcgc ggtatcgatc
    61021 tcacggcgat gatggcgcag ggcattacga tgggcgatga gttccatcaa cgcaatattg
    61081 cttcctctgc actgttaatg cgtgcgctgg ccccacaaat tgctcgcctc gatcatgata
    61141 aacagcacat cgccgaagtg atggatttcc tcagcgtgac cgatcagttc ttcctcaacc
    61201 tcgcgatggc ttactgcaag gcggcgatgg atgctggcgc gatgatccgc gcaggcagca
    61261 tcgtcacggc aatgacccgc aacggcaata tgttcgggat ttgggtaagc gggctgggcg
    61321 aacgctggtt tactgcgcct gtaaacactc cgcaaggtct gtttttcacc ggcttctcgc
    61381 aggagcaggc gaacccggat atgggcgata gcgcgattac cgaaaccttt ggtatcggag
    61441 gtgcggcaat gatcgcagcg cctggcgtaa cgcgctttgt cggtgcgggt ggcatggaag
    61501 cggccagagc ggtatctgaa gagatggcgg aaatttacct tgagcgcaat atgcagttgc
    61561 agatcccagg ctgggatttt cagggcgcgt gcctgggact ggacattcgt cgcgtggtag
    61621 aaaccggcat tacgccactc atcaataccg gtatcgccca taaagaggcg gggatcgggc
    61681 agattggcgc aggcactgtg cgggcaccgc tggcgtgttt tgaacaggcg ctggaagcac
    61741 tggctgaaag catggggatt ggttgaggaa cgcgcaatga cgatcatcca tcctctgctt
    61801 gccagtagta gcgcaccgaa ttatcgccag tcctggcggt tagcgggagt gtggcggcgg
    61861 gcgattaacc tgatgacgga aagcggcgaa ctgttaacgc tgcatcgtca gggtagtggt
    61921 ttcggccccg gaggatggat gcttcgccgt gcgcaattcg atgcgttatg cggtggatta
    61981 tgcggcaatg aacgaccaca ggttgtggct caagggattc gcctcgggcg tttcacggtt
    62041 aaacagccac agcgttattg tttgctgcgt attacgccgc ctgcgcatcc tcaaccactt
    62101 gcagctgcat ggatgcaacg cgcggaggaa accgggcttt tcgggccact ggcgttggcg
    62161 gcaagcgatc cgctgcctgc tgagttacgc cagtttcgtc actgttttca ggccgcgctc
    62221 aatggcgtta agaccgactg gcggcactgg ctgggtaaag gccccggatt aacgccgagt
    62281 catgatgaca cgctgagcgg aatgctgctg gcggcctggt attatggcgc tttagatgcg
    62341 cgctccggtc gccagttttt tgcctgttcc gacaatctgc aactcgttac cacagcggtg
    62401 agcgtcagtt atttacgtta tgccgcgcaa ggatatttcg cctcgccact cctgcacttt
    62461 gttcatgctc tgagttgccc gaaacgtacc gctgttgcga ttgattcgct gctggcgctg
    62521 gggcatacgt caggggcaga tacactgctg gggttctggc ttggtcaaca attattacaa
    62581 ggaaaaccat gaaaacactg gttgtagctc ttgggggcaa cgccttactc cagcgcggtg
    62641 aggcgctgac ggcagaaaat caatatcgca atatcgccag tgctgtaccc gcgctggcgc
    62701 gcctggcccg ttcttatcgg ctggcgattg ttcacggcaa cgggccgcag gtggggctgc
    62761 tggcattgca gaatctggcg tggaaagagg tagaaccgta tccgctggat gtgctggtgg
    62821 cagaaagtca ggggatgatt ggctatatgc tggcgcagag tttgagcgca cagccgcaga
    62881 tgccgcccgt gacgacggtg ctgacgcgca ttgaggtttc gcctgatgat ccggcgtttt
    62941 tgcagccaga gaaatttatt ggtccggttt atcagccaga agaacaagag gcactggaag
    63001 cggcttacgg ctggcagatg aaacgtgatg gtaaatattt gcgccgggtg gtggcgtctc
    63061 cgcaaccgcg taaaattctc gacagcgaag ccatcgagtt gttgctcaaa gaggggcatg
    63121 tggtgatttg cagtggcggc ggcggtgtgc ctgtgacgga agatggggca gggagtgaag
    63181 cggtgattga taaagatctc gctgctgcgt tgctcgctga gcagattaac gccgatgggc
    63241 tggtgatcct cactgatgct gatgcggtat atgaaaactg gggaacgccg cagcaacgcg
    63301 ccattcgcca tgccacaccg gatgagttag cgccatttgc caaagccgat ggttcgatgg
    63361 ggccgaaggt aacggcggtg agtggttatg tcagaagccg tggtaaaccc gcgtggattg
    63421 gggcgttatc gcgaattgaa gagacgctgg cgggcgaagc ggggacctgt atttcgctgt
    63481 agtgatgaga gcatccagca atcggtgcac catgcctgat gcgacgcttg acgcgtctta
    63541 tcaggcctac aatgggtacc ggatcggtgt aggccggata aggcgttcac gccgcatccg
    63601 gcaatcaaat gagcaccagg ttaactgaac ttactctgtg cccacatcac gccgctggca
    63661 tattccggcg gcagcagcgg gattaaggct tccagcgtcg cggacagacg cgatgtgtcg
    63721 ctgtcggtca aattcagatg ccccacttta cgccccggac ggacttcttt gtcgtaccag
    63781 tgcagatgca ccagcggcaa tttcagccag tcataattca catcgctacc aatcagattg
    63841 atcatcaccg acggactatt cactactggt tgcggtaacg gcagatcggt aatcgcccgc
    63901 agatgcagct caaactggct gatgctggca ccgttttgtg tccagtgacc gctgttatgc
    63961 acacgcggag ccagttcgtt gatcagcaga ccttgcgggg tgacaaaaca ctccatcgcc
    64021 atcacgccca catagcccag ctcctgcata atcgccgaca gcatctcttc ggcttgcgcc
    64081 tgctgctgtg cgttggcctg cggaaaagcg acgctggtgc gcaaaatacc gtcctgatgc
    64141 aggttatgcg tcagcggata aaacacggtg ctgccatcaa agccgcgcgc gccaaccagc
    64201 gacacttcac cagagaagtt aatgccctgc tcgacaatac attcgccgta acactctgcc
    64261 ggtaactgtt cggtttcatt tgcgcgtaaa cgccattgac cgcgaccgtc ataaccacca
    64321 gtgcgacgct taacaatcgc cagctcacct aaacgatcaa acaccgcagg ccactcgctg
    64381 cgttcggcaa gtaactgcca cggtgcagtc ggcaggtgga gcttatcgaa aagctgcttc
    64441 tgagtcagac ggtcagcaat aatcgggaac acatcgcggt tcacaaaggc cggatggcgc
    64501 gccagctcgc gggttaatgc ggtttccggc cagcgttcta tctcagcggt aatcacgctt
    64561 tgttgaaaag gcaccgccgc cggttcagcg tccagcccga ctggccagac agcaatgcct
    64621 aacggttcgc ctgcctgacg cagcatacgg cctaactgcc cgttaccgag gacgcaaacc
    64681 tgtttcatgc cgcacctcgc gggtccgggt tttccagcac ttcgtcggtc tgggctttgc
    64741 gccagtcatt cagacgctgg tgcagttctt tatcatgagt cgcaagaatt tgtgctgcca
    64801 gtaacgccgc gtttgccgcg ccagctttac caatcgccag cgtacccacc ggaatgccgc
    64861 gcggcatttg tacgatggag tagaggctat cgacaccgct cagtgcggcg ctctgtactg
    64921 gcacgcccag caccggcacc agcgttttgg cggcaatcat gcctggcaga tgcgctgcgc
    64981 cgcctgcgcc cgcaataatc acctgataac cgttctcttc ggcgctttcg gcgaagctga
    65041 acagtttatc gggggtgcgg tgagcagaaa ccacttcaac gtggtgcggg acattcagga
    65101 tttcgaagat ttcggcggcg aactgcatgg tagcccagtc gcttttggac cccatcacga
    65161 tggcgacacg cgccggatta ttgcgggaag acatgcgtct taaaactcct gtggtgcaca
    65221 actctcggct ttagagggca cagagaatag cacggaaaga gagcaaggaa aacggttgcg
    65281 tggctgtgaa atcagcaaag ttgcgggttt tttaaaacgg aaaatgaatt agctcaacgt
    65341 catccgccgt gactttcacc atcgaacctt ccgtatgcca ggcacccagt accacgcgaa
    65401 aagcaggttg ctgattggcg ataagttcat gcaccgccgg gcgatgggta tgcccgtgga
    65461 tcagcccttg cacctgatgt ttttccatcg cactgactac cgcgttttgg ttaacgtcca
    65521 tgatcgccag cgatttactg ctattggctt ctttactgtt cgcgcgcatt cgcgcggcaa
    65581 tgcgtttgcg cacaaacaac ggcagagcga ggaatagcat ttgcagccag ggtttgtgga
    65641 ctttggcgcg aaaagcctga taacccgcgt catcggtgca cagcgtgtcg ccatgcataa
    65701 tcaacacccg gcgaccataa agttcgagca ccttttcttc cggcaataat gtcatgccgc
    65761 tttcacgggc aaagcgtttg ccgagcagaa aatcacggtt gccatgaatg aaataacagg
    65821 gaacgccgga atcggacacc gctttgatcg ctgccgccat ctggcgatgg agtgggttgg
    65881 gatcgtcgtc gccaatccat gcttcaaaca gatcgccaag aatatacagc gcgtcggcct
    65941 tgcgggcttc ccccgctaaa aaacgcagaa aaccggcggt gatcgccggg tcttccacgc
    66001 agagatgaag atctgcaata aagagtgtcg ccacgattac tcgctaacgg tcacgctttc
    66061 aatgataacg tcttcttttg gcacgtcctg gtgcataccg ctacgaccgg ttgcaacacc
    66121 tttgattttg tctaccacgt ccatgccgtc aaccacttca gcaaacacgc agtagcccca
    66181 accttgcagg ctttcgccag agaagttcag gaagtcgtta tcaaccacgt tgatgaagaa
    66241 ctgtgcagtt gcagagtgcg gagcctgagt acgtgccatt gccagcgtac cacgggtatt
    66301 tttcaggccg ttgttggctt cgtttttgat cggttctttg gtggcttttt gtttcatgcc
    66361 cggttcaaaa ccgccgccct gaatcataaa gccgttgata acacggtgga aaatggtgtt
    66421 gttgtaaaaa ccttcgcggc agtagtccag gaagttttta actgtttcag gtgctttatc
    66481 gtcaaaagtt ttgatgacaa tatcgccgtg attggtgtgg aaagtaacca tttttgcatc
    66541 ctgttccgtt tgattggtgc ttcaacccag ttcgggtcat atatagggtg gtgttatagc
    66601 ataaccgcac gatcggatca tcacgcaatg tatgctgatt cgcgcgggaa atatgggtat
    66661 tatacgcaac tcaattaccc acacatgtct aaacggaatc ttcgatgcta aaaatcttca
    66721 atactctgac acgccaaaaa gaggaattta agcctattca cgccggggaa gtcggcatgt
    66781 acgtgtgtgg aatcaccgtt tacgatctct gtcatatcgg tcacgggcgt acctttgttg
    66841 cttttgacgt ggttgcgcgc tatctgcgtt tcctcggcta taagctgaag tatgtgcgca
    66901 acattaccga tatcgacgac aaaatcatca aacgcgccaa tgaaaatggc gaaagctttg
    66961 tggcgctggt ggatcgcatg atcgccgaaa tgcacaaaga ttttgatgcg ttgaacattc
    67021 tgcgcccgga tatggagccg cgcgcgacgc accatatcgc agaaattatt gaactcactg
    67081 aacaactgat cgccaaaggt cacgcttatg tggcggacaa cggcgacgtg atgttcgacg
    67141 tcccgaccga tccaacttat ggcgtgctgt cgcgtcagga tctcgaccag ctgcaggcag
    67201 gcgcgcgcgt tgacgtggtc gacgacaaac gcaacccaat ggacttcgtt ctgtggaaga
    67261 tgtcgaaaga gggcgaaccg agctggccgt ctccgtgggg cgcgggtcgt cctggctggc
    67321 acattgaatg ttcggcaatg aactgcaagc agctgggtaa ccactttgat atccacggcg
    67381 gcggttcaga cctgatgttc ccgcaccacg aaaacgaaat cgcgcagtcc acctgtgccc
    67441 atgatggtca gtatgtgaac tactggatgc actcggggat ggtgatggtt gaccgcgaga
    67501 agatgtccaa atcgctgggt aacttcttta ccgtgcgcga tgtgctgaaa tactacgacg
    67561 cggaaaccgt gcgttacttc ctgatgtcgg gccactatcg cagccagctg aactatagcg
    67621 aagagaacct gaagcaggcg cgtgcggcgc tggagcgtct ctacactgcg ctgcgcggca
    67681 cagataaaac cgttgcgcct gccggtggcg aagcgtttga agcgcgcttt attgaagcga
    67741 tggacgacga tttcaacacc ccggaagcct attccgtgct gtttgatatg gcgcgtgaag
    67801 taaaccgtct gaaagcagaa gatatggcag cggcgaatgc aatggcatct cacctgcgta
    67861 aactttccgc cgtattgggc ctgctggagc aagaaccgga agcgttcctg caaagcggcg
    67921 cgcaggcaga cgacagcgaa gtggctgaga ttgaagcgtt aattcaacag cgtctggatg
    67981 cccgtaaagc gaaagactgg gcggcggcag atgcggcgcg tgaccgtctt aatgagatgg
    68041 ggatcgtgct ggaagatggc ccgcaaggga ccacctggcg tcgtaagtaa ttgcgctatt
    68101 gccggatgcg agttttcgca tccggttatc gtctgcgcca ccacaacatt cccatcagta
    68161 gcatccccgg caaccacacc cacatcaatt cagaaataat cacctgatgc ccgtacgctg
    68221 tggtgtaacg agacaatgca aacggcgcga ctttaatcac ctgccaggga gcgaaaaagc
    68281 gttcatctga ccacggccac agccagccaa cccctttacc gccagtggtt accgaatcca
    68341 gcaagctgtg cgatagcaac gagacggtta aaaacagcca gcagcgaatc agcccagccc
    68401 tgaaccatcg acgtccaatc agcacacata acagcgggac aacaaacgca aacaccagcg
    68461 aatgggtaaa cccgcgatga ccaaaaacat tgccgtaagc aacgccaaaa ttaaacgaca
    68521 agacgtcggc atcgggcagc atcgccagga tgattccggc aaataacagg cgcggaggga
    68581 tgactttcga acccaaccct aaaccaatgc ataggggaac ggcggcgtgc gtaataacgg
    68641 ttggcatgat ggtcgcttcg gcaaaatgtc gatgctatca gcatggatga acggggcgta
    68701 gagggcaaaa gtctgaaaag agaaccggcc tgttgataca ggccgggaaa gggatcaggc
    68761 aacaacctgt acgctgtgac ctgcaaaact cactgtctga ccggcgacga ttttgcagcg
    68821 tttgcgcgtt tcaaccgcac cgtcgacttt cacctggcct tcggcaatcg cgattttcgc
    68881 ctgcgcgccg ctttcgctcc agccttccag tttcagcaag tcgcacagct caacgtgcgg
    68941 atgtttacct aaagaaaatg tcgccatgtt actcatcctg tggatcatga tattcaacgc
    69001 acgcctgtag cgtgttttca atcagcgtgg caaccgtcat cgggccaacg ccgccgggaa
    69061 caggcgtaat gtatgaggcg cgtttagccg cgtcttcaaa cacgacgtcg cccacaactt
    69121 tgccattttc cagacggttg atgccgacat caatcacaat tgcgccttct ttgatccagt
    69181 caccgggaat aaagcctggc ttgccaacgg caacgatcaa tagatcggca ttttctacgt
    69241 gatgacgcag atttttagtg aagcggtgag tcactgtagt ggtgcaacct gccagcagca
    69301 gttccatgct catcgggcgg ccaacgatat tcgatgcgcc aatcaccacg gcgttgaggc
    69361 cgaaggtatc aatgttgtaa cgctcaagca gcgtgacgat accgcgcggg gtgcagggac
    69421 gcagacgcgg cgcgcgctgg cacagacgac cgacgttgta aggatggaaa ccgtccacgt
    69481 ctttgtccgg atgaatacgt tccagcactt tgacgttatc aatacccgcc ggtaacggca
    69541 gttgaaccag aatgccatcg atggtgttgt cggcattcag cgtatcgata agctccagca
    69601 gctccgcttc gctggtggtt tccgggaggt cataagagcg ggagacgaac ccgacttctt
    69661 cacaagcctt gcgtttgctt gcgacataaa tttgcgatgc agggttacta cccaccagca
    69721 caacggccag tcctggtgcc cgcagtccgg ctgcaatacg cgcctgaact ttttgagcaa
    69781 cttcagagcg cacctgctgc gcaatcgttt taccgtcaat aatctttgct gccatcagag
    69841 agaggattcc atctgttacg taggtcgaag gggatgcgcc tattttgtca gaagcggggc
    69901 gcgctgtcag gtttcgtttc agatttatcg cgtgaagcga cctcttgcga aggtgaggcg
    69961 caccgtcgct gagactgaaa gcttcatttt tcgtccatga tggcgttgta aatctggaac
    70021 tgatttattt ccttgtcta
//
LOCUS       DAJOWB010000011        69898 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJOWB010000011 DAJOWB010000000
VERSION     DAJOWB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.011"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(168..785)
                     /locus_tag="OHO85_000814"
     CDS             complement(168..785)
                     /locus_tag="OHO85_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HCQ2670237.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            1052..2551
                     /gene="ansP"
                     /locus_tag="OHO85_000815"
     CDS             1052..2551
                     /gene="ansP"
                     /locus_tag="OHO85_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCQ2670238.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTALVVEEDEEKQEIVFKPETAS"
     gene            complement(2666..3727)
                     /locus_tag="OHO85_000816"
     CDS             complement(2666..3727)
                     /locus_tag="OHO85_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCQ2670239.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3969..6071
                     /locus_tag="OHO85_000817"
     CDS             3969..6071
                     /locus_tag="OHO85_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCQ2670240.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTSVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNLGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(6107..6772)
                     /gene="mcbR"
                     /locus_tag="OHO85_000818"
     CDS             complement(6107..6772)
                     /gene="mcbR"
                     /locus_tag="OHO85_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCQ2670241.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6970..8007)
                     /gene="curA"
                     /locus_tag="OHO85_000819"
     CDS             complement(6970..8007)
                     /gene="curA"
                     /locus_tag="OHO85_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCQ2670242.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPNYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8188..8706
                     /gene="mddA"
                     /locus_tag="OHO85_000820"
     CDS             8188..8706
                     /gene="mddA"
                     /locus_tag="OHO85_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCQ2670243.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8703..9152
                     /locus_tag="OHO85_000821"
     CDS             8703..9152
                     /locus_tag="OHO85_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCQ2670244.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(9153..9386)
                     /locus_tag="OHO85_000822"
     CDS             complement(9153..9386)
                     /locus_tag="OHO85_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCQ2670245.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9472..9645)
                     /gene="ortT"
                     /locus_tag="OHO85_000823"
     CDS             complement(9472..9645)
                     /gene="ortT"
                     /locus_tag="OHO85_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCQ2670246.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9840..9935
                     /gene="yncL"
                     /locus_tag="OHO85_000824"
     CDS             9840..9935
                     /gene="yncL"
                     /locus_tag="OHO85_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCQ2670247.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(10032..11456)
                     /gene="patD"
                     /locus_tag="OHO85_000825"
     CDS             complement(10032..11456)
                     /gene="patD"
                     /locus_tag="OHO85_000825"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCQ2670248.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(11478..12272)
                     /locus_tag="OHO85_000826"
     CDS             complement(11478..12272)
                     /locus_tag="OHO85_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2670249.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(12262..13203)
                     /locus_tag="OHO85_000827"
     CDS             complement(12262..13203)
                     /locus_tag="OHO85_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2670250.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPSVLIALYLAFVKRLGAFDAL"
     gene            complement(13204..14217)
                     /locus_tag="OHO85_000828"
     CDS             complement(13204..14217)
                     /locus_tag="OHO85_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2670251.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGTISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            complement(14235..15380)
                     /locus_tag="OHO85_000829"
     CDS             complement(14235..15380)
                     /locus_tag="OHO85_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCQ2670252.1"
                     /translation="MSKTFARSSLCALTMTIMNAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            complement(15625..17031)
                     /locus_tag="OHO85_000830"
     CDS             complement(15625..17031)
                     /locus_tag="OHO85_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCQ2670253.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(17110..17526)
                     /gene="hicB"
                     /locus_tag="OHO85_000831"
     CDS             complement(17110..17526)
                     /gene="hicB"
                     /locus_tag="OHO85_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HCQ2670254.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            complement(17572..17726)
                     /locus_tag="OHO85_000832"
                     /pseudo
     CDS             complement(17572..17726)
                     /locus_tag="OHO85_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            17948..18178
                     /locus_tag="OHO85_000833"
     CDS             17948..18178
                     /locus_tag="OHO85_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HCQ2670255.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQL"
     gene            complement(18270..20231)
                     /gene="rlhA"
                     /locus_tag="OHO85_000834"
     CDS             complement(18270..20231)
                     /gene="rlhA"
                     /locus_tag="OHO85_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCQ2670256.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(20304..20840)
                     /gene="sutR"
                     /locus_tag="OHO85_000835"
     CDS             complement(20304..20840)
                     /gene="sutR"
                     /locus_tag="OHO85_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCQ2670257.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            20893..22107
                     /gene="ydcO"
                     /locus_tag="OHO85_000836"
     CDS             20893..22107
                     /gene="ydcO"
                     /locus_tag="OHO85_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCQ2670258.1"
                     /translation="MLRCLYAITYEVTMRLFSIPPPTLLAGFLAVLIGYASSGAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            complement(22147..23355)
                     /locus_tag="OHO85_000837"
     CDS             complement(22147..23355)
                     /locus_tag="OHO85_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2670259.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     VGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     AYCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQ
                     EPTEMIQATA"
     gene            23363..23869
                     /gene="tnpA"
                     /locus_tag="OHO85_000838"
                     /pseudo
     CDS             23363..23869
                     /gene="tnpA"
                     /locus_tag="OHO85_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(23887..24555)
                     /locus_tag="OHO85_000839"
     CDS             complement(23887..24555)
                     /locus_tag="OHO85_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCQ2670260.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPISYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(24857..25450)
                     /gene="tehB"
                     /locus_tag="OHO85_000840"
     CDS             complement(24857..25450)
                     /gene="tehB"
                     /locus_tag="OHO85_000840"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCQ2670261.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(25447..26439)
                     /gene="tehA"
                     /locus_tag="OHO85_000841"
     CDS             complement(25447..26439)
                     /gene="tehA"
                     /locus_tag="OHO85_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCQ2670262.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVMSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            26563..27543
                     /locus_tag="OHO85_000842"
     CDS             26563..27543
                     /locus_tag="OHO85_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HCQ2670263.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(27538..28074)
                     /gene="rimL"
                     /locus_tag="OHO85_000843"
     CDS             complement(27538..28074)
                     /gene="rimL"
                     /locus_tag="OHO85_000843"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCQ2670264.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLSKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(28137..28361)
                     /locus_tag="OHO85_000844"
     CDS             complement(28137..28361)
                     /locus_tag="OHO85_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCQ2670265.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(28501..30156)
                     /gene="opgD"
                     /locus_tag="OHO85_000845"
                     /gene_synonym="mdoD"
     CDS             complement(28501..30156)
                     /gene="opgD"
                     /locus_tag="OHO85_000845"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCQ2670266.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(30381..31724)
                     /locus_tag="OHO85_000846"
     CDS             complement(30381..31724)
                     /locus_tag="OHO85_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCQ2670267.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            31941..32864
                     /locus_tag="OHO85_000847"
     CDS             31941..32864
                     /locus_tag="OHO85_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2670268.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEDLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(32902..34542)
                     /gene="trg"
                     /locus_tag="OHO85_000848"
     CDS             complement(32902..34542)
                     /gene="trg"
                     /locus_tag="OHO85_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCQ2670269.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            34941..35090
                     /gene="hokB"
                     /locus_tag="OHO85_000849"
     CDS             34941..35090
                     /gene="hokB"
                     /locus_tag="OHO85_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCQ2670270.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(35162..35335)
                     /locus_tag="OHO85_000850"
     CDS             complement(35162..35335)
                     /locus_tag="OHO85_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670271.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGEISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(35580..36110)
                     /gene="cybB"
                     /locus_tag="OHO85_000851"
     CDS             complement(35580..36110)
                     /gene="cybB"
                     /locus_tag="OHO85_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCQ2670272.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            36299..37300
                     /gene="gap"
                     /locus_tag="OHO85_000852"
     CDS             36299..37300
                     /gene="gap"
                     /locus_tag="OHO85_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCQ2670273.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDNTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(37342..38781)
                     /gene="aldA"
                     /locus_tag="OHO85_000853"
     CDS             complement(37342..38781)
                     /gene="aldA"
                     /locus_tag="OHO85_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCQ2670274.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVDGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(38978..39778)
                     /locus_tag="OHO85_000854"
     CDS             complement(38978..39778)
                     /locus_tag="OHO85_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCQ2670275.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(40050..43952)
                     /gene="hrpA"
                     /locus_tag="OHO85_000855"
     CDS             complement(40050..43952)
                     /gene="hrpA"
                     /locus_tag="OHO85_000855"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCQ2670276.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEAVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            44153..44758
                     /gene="azoR"
                     /locus_tag="OHO85_000856"
     CDS             44153..44758
                     /gene="azoR"
                     /locus_tag="OHO85_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCQ2670277.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSVDEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(44809..46125)
                     /locus_tag="OHO85_000857"
     CDS             complement(44809..46125)
                     /locus_tag="OHO85_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCQ2670278.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(46115..47872)
                     /locus_tag="OHO85_000858"
     CDS             complement(46115..47872)
                     /locus_tag="OHO85_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCQ2670279.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRALAFEKMQ
                     SFISHLYANKSQKFDYQHEDCTGPSADRWRLLSGGPVPLSPVDLAYRFMRMAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLVDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVADLHAKGLAIRVVDIAAGHGRYVLDALENEPAVCDILLRDYSELNVAQGQE
                     MIAQRGMSGQVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(47888..48784)
                     /locus_tag="OHO85_000859"
     CDS             complement(47888..48784)
                     /locus_tag="OHO85_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCQ2670280.1"
                     /translation="MLEKSLATLFALLILATLINHFLLWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYSCY"
     gene            complement(48784..49389)
                     /locus_tag="OHO85_000860"
     CDS             complement(48784..49389)
                     /locus_tag="OHO85_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCQ2670281.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(49688..50548)
                     /gene="pdxI"
                     /locus_tag="OHO85_000861"
     CDS             complement(49688..50548)
                     /gene="pdxI"
                     /locus_tag="OHO85_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HCQ2670282.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRIMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(50779..51369)
                     /gene="paaY"
                     /locus_tag="OHO85_000862"
     CDS             complement(50779..51369)
                     /gene="paaY"
                     /locus_tag="OHO85_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HCQ2670283.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            complement(51351..52301)
                     /gene="paaX"
                     /locus_tag="OHO85_000863"
     CDS             complement(51351..52301)
                     /gene="paaX"
                     /locus_tag="OHO85_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HCQ2670284.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVVDNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(52402..53715)
                     /gene="paaF"
                     /locus_tag="OHO85_000864"
     CDS             complement(52402..53715)
                     /gene="paaF"
                     /locus_tag="OHO85_000864"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HCQ2670285.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(53742..54947)
                     /gene="paaJ"
                     /locus_tag="OHO85_000865"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(53742..54947)
                     /gene="paaJ"
                     /locus_tag="OHO85_000865"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HCQ2670286.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(54947..55369)
                     /gene="paaI"
                     /locus_tag="OHO85_000866"
     CDS             complement(54947..55369)
                     /gene="paaI"
                     /locus_tag="OHO85_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HCQ2670287.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(55359..56786)
                     /gene="paaC"
                     /locus_tag="OHO85_000867"
     CDS             complement(55359..56786)
                     /gene="paaC"
                     /locus_tag="OHO85_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaC"
                     /protein_id="HCQ2670288.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAANLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGGNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(56788..57576)
                     /gene="paaB"
                     /locus_tag="OHO85_000868"
     CDS             complement(56788..57576)
                     /gene="paaB"
                     /locus_tag="OHO85_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase"
                     /protein_id="HCQ2670289.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(57576..58343)
                     /locus_tag="OHO85_000869"
     CDS             complement(57576..58343)
                     /locus_tag="OHO85_000869"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase"
                     /protein_id="HCQ2670290.1"
                     /translation="MSELIVSRQQRVLLLTLNRPAARNALNNALLMQLVNELEAAATD
                     TSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(58340..59410)
                     /gene="paaK"
                     /locus_tag="OHO85_000870"
     CDS             complement(58340..59410)
                     /gene="paaK"
                     /locus_tag="OHO85_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HCQ2670291.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYARDHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIAATLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNAGDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(59418..59915)
                     /gene="paaD"
                     /locus_tag="OHO85_000871"
     CDS             complement(59418..59915)
                     /gene="paaD"
                     /locus_tag="OHO85_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate degradation protein PaaD"
                     /protein_id="HCQ2670292.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMSTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVCCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(59930..60676)
                     /gene="paaC"
                     /locus_tag="OHO85_000872"
     CDS             complement(59930..60676)
                     /gene="paaC"
                     /locus_tag="OHO85_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HCQ2670293.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(60685..60972)
                     /gene="paaB"
                     /locus_tag="OHO85_000873"
     CDS             complement(60685..60972)
                     /gene="paaB"
                     /locus_tag="OHO85_000873"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HCQ2670294.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(60984..61913)
                     /gene="paaA"
                     /locus_tag="OHO85_000874"
     CDS             complement(60984..61913)
                     /gene="paaA"
                     /locus_tag="OHO85_000874"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HCQ2670295.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            62198..64243
                     /gene="paaZ"
                     /locus_tag="OHO85_000875"
     CDS             62198..64243
                     /gene="paaZ"
                     /locus_tag="OHO85_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HCQ2670296.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RLFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDEAPAVH
                     ATEAFGPVATLMPAQNQRHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            64491..66764
                     /gene="tynA"
                     /locus_tag="OHO85_000876"
     CDS             64491..66764
                     /gene="tynA"
                     /locus_tag="OHO85_000876"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HCQ2670297.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQLFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDSRDRVAPAVK
                     PMQIIEAEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(66822..68321)
                     /gene="feaB"
                     /locus_tag="OHO85_000877"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(66822..68321)
                     /gene="feaB"
                     /locus_tag="OHO85_000877"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HCQ2670298.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGDYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            68557..69462
                     /gene="feaR"
                     /locus_tag="OHO85_000878"
     CDS             68557..69462
                     /gene="feaR"
                     /locus_tag="OHO85_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HCQ2670299.1"
                     /translation="MNPAMDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(69634..>69898)
                     /locus_tag="OHO85_000879"
     CDS             complement(69634..>69898)
                     /locus_tag="OHO85_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCQ2670300.1"
                     /translation="LTLDEARTQGRVGETFYGYLVALKTDAETEKLVTDINAERKASY
                     QQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGINGKWVRKF"
BASE COUNT        17358 a        18317 c        16848 g        17375 t
ORIGIN      
        1 tgcacaatgt gaatagttag tttcttttca gaggaaatga aatagacaag ctgaatgaag
       61 gggagagtga tataaatcaa aaaacataac atgaatttaa ttaattacgc ccatcattgt
      121 ggataaagtt actgaataat aaataacata atcctctggt atcccactca aataatttca
      181 ttccttttta atacttcttg taatttcggt aactgacata cagcatcagc aatcgcactg
      241 atattcgtgg cattatcctg aaaccattca tgcccaggcc cccatgtgcg catagtgcaa
      301 agataacagt ccaccagcgt tagttgctca ccaaacgcat aaggttcagc ggtcaattgt
      361 gaattcagcc agatataaag cgatttacga tattcaataa cattcttctt taactgttca
      421 ggtgcgtcag gagcccaacg ctcagggtaa tcggcgaaag tgaatgttgg atagacatta
      481 gcaaccagcc agaccaatag ccgttgaaac tgctgacgtt cggcgcgccc aaccggtggc
      541 gcaaggtccg ggcgacgatc gagaaccatc aatgcaatcg ccgctgtctc cgtcataatc
      601 tcgtcatttt ccagcgccag tgtcggaatc tgacacaacg ggttcagggt tttcaataac
      661 tcgcgtgaag ttccctcgtg gtcaaaacca ctgacatcaa cgaactgata aggaatatca
      721 gccagtgtta gcatcagctc actgattgta gagccccatc cgggtacgcc atacacctta
      781 atcatatgcc cctcacaatc ttttaaagat taagtgtagg acagtacaca ggttaaccag
      841 caaaaggatt tgtcattttt tcgtcagttg tattaatccc cttcaggatt gtaaggggat
      901 gttttgcatc gtttatcgat aacgggtagg atgctgcgcc gatagtttta tacccataaa
      961 gaataatggt gataactatc atcgccagga tgaataaaca ttgttcatgg caacttatat
     1021 gactttttca ttaaagcaat cagggagagc aatgagtaaa cacgacaccg acacttcaga
     1081 tcaacacgcc gcgaaacgcc gctggcttaa tgcccacgaa gaggggtatc acaaagcgat
     1141 gggcaatcgc caggtacaga tgatcgccat tggcggcgcg attggcaccg gcttgttttt
     1201 aggtgcagga gcccgactgc aaatggcggg tccagcactg gcactggttt atttaatttg
     1261 tggcttgttt tcgtttttta ttctgcgtgc attgggtgag ctggtgctac accgcccttc
     1321 cagtggcagt tttgtctctt atgcccgtga gtttttgggt gagaaagccg cttatgttgc
     1381 tggctggatg tacttcatca actgggcgat gacggggatt gttgatatta ccgccgtcgc
     1441 tctgtatatg cattactggg gtgcgtttgg cggcgtgccg cagtgggtct ttgcgctcgc
     1501 tgcacttacc atcgttggca ccatgaatat gatcggtgtg aaatggtttg cggagatgga
     1561 gttctggttt gcgcttatta aagtgctcgc cattgtgacc tttttggtcg tgggtacagt
     1621 gttcctcggt agtggtcagc cgctggatgg caacaccact ggctttcatt taatcaccga
     1681 taatggcggc ttcttccccc acggtttgct gcctgcgctg gtgttgattc agggcgtagt
     1741 gtttgctttt gcctccattg aaatggtggg tacagctgcc ggagaatgta aagatccgca
     1801 gaccatggtg cctaaagcga ttaacagcgt gatttggcgt attggcctgt tttacgtcgg
     1861 ctccgtggtg ttgctggtta tgctattgcc gtggagcgcg tatcaggcgg ggcaaagtcc
     1921 gttcgtgacg tttttctcta aactgggtgt gccatatatc ggcagcatta tgaacattgt
     1981 ggtgctgacc gctgccctct ccagcctgaa ctcaggtctg tactgcaccg gacgtattct
     2041 gcgctcaatg gcgatgggcg gttccgcacc gagttttatg gcgaaaatga gtcgtcagca
     2101 tgtgccgtat gccgggattc tagcaacact agttgtgtat gtcgtcggcg tattcctcaa
     2161 ctatctggtg ccgtcgcgcg tatttgagat tgtgttgaac ttcgcgtcgc tgggaatcat
     2221 cgcttcatgg gcgtttatca tcgtgtgcca gatgcgcctg cgtaaagcga ttaaagaagg
     2281 caaagcagcg gatgtcagtt ttaaactgcc tggcgcgccc ttcacttcct ggctgacatt
     2341 actgttttta ctgagtgtcc ttgtgctgat ggcgttcgat tacccgaacg ggacttatac
     2401 tatcgcggcg ctgccgatta tcggtattct gctggttata ggctggtttg gtgtgcgcaa
     2461 acgcgttgct gaaattcaca gcactgcgct agtcgtcgag gaagatgaag aaaaacagga
     2521 aatcgtgttt aagcctgaaa cggcgagcta attgcaaaat tgcccggcta cgtgccgggc
     2581 aatttcaaat tactgacgaa ggtgtgccgc atgcggcgtg ataaaagtca ggcaagtctc
     2641 tccgttttca ccctcgccat atctattaca gcgcaatacg aatcacatcg tccggctggg
     2701 tagcttcctg ctgtttagtg gatttttgtt ttacactgac atacagcgtt ttgccatcgg
     2761 cagacagcgc caggctgttc ggatgagtcg gcgtatcgaa cgttttcacc actttatagc
     2821 ttttcgcgtc aatcacactg actttacctg cctgacgatg cgttacgtag gcttcattac
     2881 gcgctgggtt aaacagcaca gccagtgatt ccggtgccgc aaccttcgcc agaatattgc
     2941 cattacgggt atcgaccact aacacttctg ccgctttaga atcggtgata aatgcacgct
     3001 gcctggcggt atcaaggcta atgttgataa agaagtgctc tttgccgtca tccagcagct
     3061 ttttacggct gaggatttta ttgtcggcgg tgtcgatggt aatcaattcg ccgtcagcgt
     3121 tagtggtgta aagacgtttg cctttgctat ccagcgccag accggtactc attttaccgg
     3181 tgttctggat ggcggttttc agtttgatat tctcgccatc aacgacccaa atcacgctct
     3241 ctttaccaat accactgatg taaacggtgt tcgtggcatc atcagctacc agctcacgcg
     3301 gttgcagcgg gcgcacctct tccgtgcgct tacgatcatc cagcaccaga cggcctttca
     3361 cttcgcccgt tttggcatct atcgccgtga ccgcgctgtt tacggtgtta ccaaaccaca
     3421 acgtctgagt cgtgttattg atggtggcac caaacggctt gagatcgtta tggatcgcct
     3481 gcgtcacttc cagagtgacc ggatcaagac gataaaccac gccaccttta tccagtttgc
     3541 ggctttgcga agtggcgagc cacagcgcgt tttcttgctg gctataagcc atttcgtagg
     3601 cacctttacc taccgcttta cgcagcattt cttctgcggc ctgcgtactg aatgatgaag
     3661 caacaagcaa tgaacctaac agtaatgaac cacgcaggcg cgatgaaaac agatgacgta
     3721 aatgcatgac gactcccttt gatgaaccaa tatttttgct gcttcacaat gcttcgctaa
     3781 gcgtaatcat cgttacgctc ttgttataaa tgggaatggt aatcattatt ttctcaaatg
     3841 tggagcattt cttgccgtta cgactacaat tttctcaatt aattaacgta tcgtgccgtt
     3901 tcgttttcca taaatttaca attttattac catgacagcg ttttcccatt cttaacctgg
     3961 cttttttcat gaagattttt tccgtccgac agaccgtttt gcccgcactg cttgtccttt
     4021 cccccgttgt ttttgccgct gatgaacaga ctatgattgt cagtgccgca ccgcaggtgg
     4081 tttccgagct ggatacgcca gcagcagtaa gcgtggtgga tggcgaggag atgcgcctgg
     4141 caacaccgcg cattaacttg tccgaatcac tgaccagcgt gcctggtttg caggtacaaa
     4201 accggcagaa ctatgcgcaa gatttacagc tgtcgattcg cggatttggc tcccgttcca
     4261 cttacggaat tcgcggtatt cgcctgtatg tggacggtat tcccgccacc atgcccgacg
     4321 ggcaagggca aacatccaac atcgatttaa gcagtgtgca aaatgtggaa gtgctgcgtg
     4381 gccccttctc tgccctgtat ggcaacgcgt ctggcggtgt aatgaatgtc accacccaga
     4441 ccggacaaca gccaccaacc attgaagcca gtagttacta cggcagtttt ggcagctggc
     4501 gctatgggct gaaagcaacg ggcgcaacgg gagacggtac acagcccggc gatgtcgatt
     4561 acaccgtctc aaccacgcgt tttacgaccc acggctatcg tgaccatagt ggcgcacaga
     4621 aaaatttagc caatgccaaa ctgggcgtac gcattgatga agccagcaaa ttaagcctga
     4681 ttttcaatag tgtggatatc aaagcagatg acccaggtgg gctaaccaaa gcagaatgga
     4741 aggcgaatcc acaacaagcg cctcgtgcag aacagtacga cacgcgaaaa accatcaagc
     4801 aaactcaggc tgggttgcgc tatgagcgta gcctgagcgc gcaagatgat atgagtgtga
     4861 tgatgtatgc cggagagcga gaaacgaccc agtaccagtc aatcccgatg gccccccaac
     4921 ttaacccgtc acatgcgggc ggcgtgatta ccctgcaacg ccattaccag gggatagaca
     4981 gccgctggac acaccgtggt gaactgggcg ttccggtcac gttcactacc ggcctgaact
     5041 acgaaaacat gagtgaaaac cgcaagggct acaataactt ccgcctgaat agcggcatgc
     5101 cggagtacgg gcaaaaaggt gagttgcgtc gcgacgaacg caatctgatg tggaacatcg
     5161 atccctattt acagacgcag tggcagctga gcgaaaaact gtcgctggat gctggcgtgc
     5221 gctacagctc cgtgtggttt gattccaacg accattacgt tactccgggt aacggcgatg
     5281 acagcggtga tgccagttat cataaatggc tacctgccgg ttcgttaaaa tatgcaatga
     5341 ccgatgcctg gaatatctat ctggcagccg ggcgaggttt tgaaacgccg acgattaatg
     5401 agctgtctta tcgtgctgat gggcaaagcg gtatgaactt aggtttaaaa ccatccacca
     5461 acgatacaat tgagatcggc agtaaaacgc gtattggtga tgggctgctt agtctcgcat
     5521 tgtttcagac cgacactgat gatgaaattg ttgtcgatag cagtagcggt gggcgtacga
     5581 cttacaaaaa tgccggaaag acccgtcgtc aaggcgctga actggcatgg gatcaacgtt
     5641 tcgcaggaga ttttcgcgta aacgcgtcct ggacctggct tgatgcgacc tatcgcagca
     5701 atgtttgcaa tgaacaggat tgtaacggta atcggatgcc agggatcgcc cgtaatatgg
     5761 gctttgcgtc gataggttat gtaccggaag atggttggta tgcaggcacg gaagcgcgtt
     5821 atatgggcga tattatggca gatgatgaaa atacggcaaa agcgccgtct tatactctcg
     5881 tcggcttatt caccgggtat aaatacaatt accacaattt aactgtggat ttatttggtc
     5941 gtgtcgataa tttattcgat aaagaatacg ttggttctgt cattgtcaat gagtcaaacg
     6001 ggcgatatta cgaaccttcg cccggacgaa attatggtgt cggcatgaat attgcgtgga
     6061 gatttgagta attaccttga tgcccggtat ttgccgggca tttactttaa cgattgtatt
     6121 gctggtataa aatagcaata ttttgttgca taatttcagc aagacaatgt ctgcatccct
     6181 ctttgtcttt tgcttttaac gcagcgagta acagatgata gttttctata tgctcccgta
     6241 attccgctgg attaatcgct tcatagagat aatgtaaacc aggccccatc ctgacccaca
     6301 gttgctcaat catctcacac aggatgggca tatttgagcg atgataaatc gctaagcgaa
     6361 atagcctgtt tacattaatg atttgttcca tgtcgccctt ttcttgcgcc tgctgtaatt
     6421 tctcgagcaa ttcctggagt tccgcaaggt cttgcggggt gaggttttca acagccagag
     6481 caactgccat taattccagt tcgtagcgga tccgattgat ttcgtctaat tgacgtttcc
     6541 ccacttccgg aactgtaaat gcttgtgcag gtgcgacaga aagcgcattc accgaaacca
     6601 gacgtaataa tgcttcacgc acaggtgtaa tactcatacc taattgctcc gccagatttt
     6661 tagtaatcag gcgtgcgcca ggttttaatg cgccaatact aagctgatgt ttcaggtcgt
     6721 tctcaacctg cagagtcaaa ctgacatgtt tcatttttcc cgttccaggc attttaattc
     6781 cttcgcgtgt tttctgtcca accctgcctg catggcagaa ggagttcacc tggtgtatta
     6841 aagtgaataa tcataaacca cccacctttg ttgttattag cagcagaaca atgacgtggt
     6901 tagttaatta aagctgaaaa taagcttttt gcctggattt ttatggcggc ttacgccgcc
     6961 gttgaaactt taatcatcac ccgcgacgcg gatcaccact ttgccgaagt ttttaccctt
     7021 cagcaagccg ataaacgtct gtggcgcatt ctctaaacca tcagtaatat cttcgtggta
     7081 gtggatttta tcctctttca cccattgccc catctccttc tgaaactcat ggatgcggtg
     7141 accataatcc tgagcgataa taaacccttg caagcgaata cgttttttca gcaccgttgc
     7201 catcaacagg ggtaaacgat ccggaccggg tggtagctct gtagcgttat agctgctcac
     7261 taagccacaa acgggaatgc gcgcagatgt attaagtaat ggtagcactg catcaaatac
     7321 cttaccgccc acgttttcat aatagatatc aatacctttt gggcacgctt tcaccagttg
     7381 ttcggcaaaa tcatccgcgt ggtgatcaag acaaacatcg aagcccagca cctcgatagc
     7441 atggcggcat ttttccgcgc caccggcaac ccccaccacc ctgcaacctt taagtttgcc
     7501 gatttgcccc accgttgctc ccacaggacc tgtcgccgca gctaccacca acgtttcgcc
     7561 ctctttaggc tgaccgatat ccagcagccc catataagcg gtaaagcctg gcatccccag
     7621 cacacccagc gaccacgatg gattttgcgg atgatcgcca agtttcacca gatcatcacc
     7681 actggatatg tcatagtcct gccatccact gtagcccagc acccagtcgc caggctgata
     7741 attaggatga ttcgactcca caacacggct caccgtgccg ccgaccatca cgccgccaat
     7801 atcaacaggc ggtgaataag atggctcatc gctcatacgt ccacgcatat acgggtccag
     7861 ggacaaataa actgtgcgca gtaacacctg tccttcaccc ggtgtggcga catcatcttc
     7921 ttcaagacgg aaattctccg gaaccggtgc gccatgtgga cgcgaggcca gaacccaacg
     7981 tcgattacgc tgcttttgtt gccccataat gctctcctgt gcttttggca tgaatccatc
     8041 aatagtagca ggctcacggg attggttttg tttgcagccg tcacatcccc tctgcaacac
     8101 gcaataattt catcgaggaa tccgttgctt cgggctgttt tcgatagtat tgataacatc
     8161 atcatcatta ttttatcgag gccagacatg tccatccgtt ttgcccgcaa agccgattgt
     8221 gctgccattg cggaaattta taaccacgcc gtgttgtata cggcggctat ctggaatgac
     8281 caaacggttg atgcagataa ccgcattgcc tggtttgaag cgcggaccat agcaggttat
     8341 ccggtgctgg taagtgaaga agatggcgta gtgacgggat atgcctcgtt tggcgactgg
     8401 cgtagtttcg atggttttcg ccataccgtg gaacattcgg tttatgtcca tcccgatcat
     8461 cagggcaaag gtctggggcg taaattgtta agccgattga ttgatgaagc gcgggattgc
     8521 gggaagcatg tcatggtcgc cgggatcgaa tcgcaaaatc aggcctcgct gcatctgcac
     8581 cattcgctgg gatttgtcgt caccgcacaa atgccgcagg tgggcactaa atttgggcgc
     8641 tggctggatc tgacatttat gcagttgcaa ctcgacgaac gcactgaacc ggacgcgatc
     8701 ggatgaatca gtcgctcacc cttgcttttt tgattgccgc cggaattggt ctggtagtcc
     8761 agaacacgct gatggtgcgc atcacccaga cctcctctac cattctcatc gccatgttgc
     8821 tgaactcact ggtggggatt gtgctctttg tttctatttt atggtttaaa caaggcatgg
     8881 cggggtttgg cgaactggtg tccagcgtgc gctggtggac acttataccc ggcttgctgg
     8941 gatcgttttt tgtcttcgcc agtatcagcg gatatcagaa tgtgggagcg gcgacaacta
     9001 ttgcagtact ggtcgccagc cagctaattg gcggtctgat gctggatatc ttccgcagtc
     9061 atggcgtacc actacgggcg ttgttcggcc caatttgtgg cgcaatttta cttgtggttg
     9121 gcgcctggtt ggtagccaga cgctcatttt aatcagagga tggtgccgcc tttggttaac
     9181 tgttcgtggc gcgcttccat atcttcttta tgcttgcgac cgtgatgagc aatagcggtg
     9241 cgcaaacgct gctgctgggt gtagcgatct tcccgactta actccgcgtc atcgctcagg
     9301 gcgattaata attcgttcat atgggcaatg acgctctctt caatcgcggc atccacgcgc
     9361 gcgatgactt catccagatg cgacatactt tctccttagc cacttgtgaa atttatcgat
     9421 tcatccaaat gatataagtc tgcgtggtgc cgtcaagcca gtgagtaaga tttaaaacag
     9481 tgaaaacaac agcgccaccg ggaaactcat cggccatgtt gcccccacca gaaaggcgct
     9541 taagaaacgg atgcgtttct tatcgtgaga aagaaaccag gtgataagaa atgcgattgc
     9601 tgccataacc gcataaaaaa caagcatgtg ttgatagaga gacatgtacg ggcgtccagc
     9661 cagatgaata aaacgcgcgc aaatatgcgt cattttttga cgtgcatcaa gttttttcac
     9721 cagtttgaga aaaaatcatc aaacagcaac ttgcccttat tcatcttcag aatgagtatt
     9781 attgcggatt ttcttaaccc gtactataca cataggggca atgataaaag gtggcaaaaa
     9841 tgaatgtttc cagtagaact gtagtactga taaatgtctt tgctgctgtt ggtttgttta
     9901 ctcttatctc tatgagattt ggctggttta tttgatgtag tgattttgca cgcagcacgt
     9961 actgtcagag aaatcagtgc tctgacagta cgcaagatca tctcatactc ttacgatctg
    10021 caatattatc cttaatgttt aaccatgacg tggcggacga cggtgtaatc ctccagccca
    10081 taaagtgaca tatccttgcc gtaaccagaa agtttctgtc caccgtgcgg catttcactt
    10141 accagcatga aatgggtatt gacccaggta caaccatatt gcagccgtgc gctgacgcga
    10201 tgcgccctgc ccacatcttt cgtccatacc gaagatgcaa gtccgtactg gctgtcattc
    10261 gcccagttca ccacctgttc ttcgttgtcg aagagcgtaa cactcactac tggaccaaat
    10321 acctcttttt gcacgatggc atcgtcctgt aatgcgccag ccagcagcgt cggcgcatag
    10381 taatagccat tacccttgcg cttttcaccg ccagtgatca ctttgatgtg ccctgtcgct
    10441 ttcgcctctt ctactgcctt gctgacgcgt tcgagatgcg ccagcgagct taaaggtcca
    10501 agctccgtag actcgtcatc cggcgcacca gattttaacg ttgccaccgc agcacccagt
    10561 ttttccacca gcgtatcgta aatgcctttt tgcgcgtaga tccgacaagc cgcagtacaa
    10621 tcctgtccag cattgtaata gccaaatgta cgtacacctt cgaccactgc ttcaatatcc
    10681 gcatcatcaa aaacaatcac tggcgctttg ccaccaagtt ccatatgagt acgcttaatg
    10741 gacgacgcgg tatggctgat gatgtgctcg ccggtggcga tagagcccgt cagcgacacc
    10801 atccgcactt tgggatgacc ggtcagcgga tcacccaccg ttttgcctct gccaaacagt
    10861 acgttaatca cgcctgccgg gaagatatct ttcgccagct ctgccaactt caacgcggtc
    10921 agcggggtaa tttctgatgg tttaagcact acgcagttcc ctgccgccag cgccggagcc
    10981 agtttccacg ctgccatcat cagcggataa ttccacggtg cgatagaagc cacgaccccc
    11041 aacggatcgc gacggatcat cgaagtatga ccttcaagat attcacctgc cgccagacca
    11101 ttcagacagc gcgccgcacc cgcgaaaaag cgaaaaacat cgacaatcgc cgggatttca
    11161 tcattgaacg cactatgcag cggtttgcca caattacggg actccagttc ggcaaaaacc
    11221 tgaccatttt cttcgataac atcagccagt ttcagcagac attccgcacg cactttcggc
    11281 gtggtttgcc cccattcggc aaatgctgca tctgccgcgc gcacagcagc atcgacctgc
    11341 tctgcggatg cctcggcaat ctccagtaaa acgtcccccg ttgccggatt atagacaggc
    11401 tgtttttccc cttcgccgct aaccagttct ccgttaatca gtaacttatg ttgcatagca
    11461 tattcctgta tcagtggtta ttgaccattg tcgccttcgc gggttagcca ccaggccccc
    11521 aggatcggca aggttgttac caacataacc agcagtgcca ccacgttagt taccggtaca
    11581 tcacgcggtc gcccaagctg attgagcaac cacaacggta acgttcgttc atgacctgcc
    11641 gtaaaagtcg taacgatgat ttcatcgaac gacaaggcaa acgccagcat tcctcctgcc
    11701 agtagcgccg aactgagatt cggcaacact acgtagcgga aggtttgcca gccattggcc
    11761 ccaagatcca ttgacgcctc aaccagactc cacgaggtgc ggcgaaaacg ggcgatgaca
    11821 ttgttaaaca ccaccactac gcaaaaagtc gcatgaccga ccacgatggt gaaaaatccc
    11881 ggctccagat tgatggtttt aaaagcggtt aatagcgcca gaccagtgac aatgcccggc
    11941 agcgcaatgg gcagcagcag taacagcgaa atggcgtttt tgccgaaaaa gtctcgtcgc
    12001 cacagcgcgg cagctgccag cgtccctaac accagcgcca ttaatgtcgc cagcgccgcc
    12061 actttaagtg acagtgtcac ggcatcaaga atatcactac gctgtgctgc cacgctaaac
    12121 cagcgcagcg tcaggccctg cggtggaaaa ctaaacgccg catcttcagt gttaaacgca
    12181 taagcggcga taatcaggat gggaaaatgt aggaaaacca cgccgcccca ggccgccagt
    12241 ttgaggaaaa acggtgcgcg ttcagagtgc atcgaacgct cccagacgtt tcacgaacgc
    12301 caggtacagt gcgatgagaa cactcggcac cagggtgaat gccgccgcca tcggcatatt
    12361 gccaatcgcc ccctgctggg aataaaccat attgccgata aaatatcctg gaggaccaac
    12421 cagctgcggg acgataaaat cgcccagtgt gagtgagaag gtaaagatag agccagcggc
    12481 aatacccggg attgccagcg gcagcaccac atagcgaaag gtttgtcgtg gacgtgcgcc
    12541 gagatcagcc gacgcctgca acaatgacgg cggcaaacgc tcaagcgccg cctgaacggg
    12601 caggatcatg aacggcaacc agatatagag aaacaccaga aagcgcccca gcccggaagt
    12661 tgacagcgta tttccgccca ccgcaggtaa tgtaaggaac gcagtcagca gtggttccag
    12721 cccaagatgt tgtaaaaacc actgagccac gccatctttt gccagcaata acgtccaggc
    12781 ataggcttta acaatgtagc tcgcccacat cggcaacatt accgcaatat aaaaaaaagc
    12841 tttcattttc ccgctggtat agcgcgccat ataccacgcc attggaaaag ccagaatggc
    12901 gctggcgata gtgactgcca cagccatagt cagcgtgcgg agaatgatgt cgtaattcgc
    12961 cggattaaac agcgcacgga tattcgccag cgtcagttcc ggcgttaccg acatggtgaa
    13021 atcgtcaaaa gtataaaatc cctgccataa cagtgtcagc agcgagccga aatagacaat
    13081 gccaaaccac attagcgggc caagcagcag taaaaacagc cccagccccg gattacgcca
    13141 gaagaaaccg gacaccttac ccagacctgg acgtgaaggt gattgcaata cattcatcgc
    13201 cattcacctc tcctcaacca gcggcaccat cacatcacgc gaccaggaaa ccatcacctg
    13261 ttgtccgggc gtgagcgtgg caggcagttc ttcgcctgtc atattggcct gactcacaag
    13321 cagtttttca ccgccgttca atttcagttc aaaacgagtt gccgcgccct gatattgcac
    13381 cgcctggatc gtgccattgg cctgcagttc accaggggtg ttgaggcgga tatgttccgg
    13441 tcgcagggcg aagcttcccg tcatgccaca aagtttctct gccatcagtc catcaaaaac
    13501 gttggatgtc ccgacgaatc cggcaacaaa cggcgtgcgc gggcgcatat agagatcgcg
    13561 cggggaatcg acctgctcaa tgcgtccatt attgaaaacc gccacacgat cggacatcga
    13621 taacgcttcg ccctgatcgt gggtaacgaa gataaaagtg ataccgagag actgttgcag
    13681 ttttttcagt tccagctgca tctgctcacg caatttgaga tccagtgcgc cgagcggttc
    13741 atccaacagc aatacgcgcg gttcattcac caatgctctg gcgatagcaa cccgctggcg
    13801 ctgaccacca gaaagttgtg acggtttacg ttgatgtaca aaccccaacg ccactttctc
    13861 cagcgcctct tgcgccattg cgtgccgctg ctttttattc acgcctttga ccatcagccc
    13921 ataggcgaca ttgtcaagaa tcgacatatg cggaaatagc gcgtagtcct gaaagacagt
    13981 attcacgtcc cgctcccacg gtggcagatt gctggcgggt ttaccaaaga tagagatagt
    14041 cccgccggaa agctgttcga agccagcaat caggcgcagg caggtggttt tgccggagcc
    14101 ggacggcccc agcatagaga agaactcacc atcttttatc gcaatactga cgccatctac
    14161 tgcgcgcacg tcaccgtaca accgcgagac gttgtcaaac tccactgcgt acgtcataaa
    14221 gcaccccagc gaagttagcg accgcccata atggcaatgt aatcctgcgt ccagcgactg
    14281 tagggaacaa acttgccccc ttctgctata ggcgttttcc agaaggcgat tttgtcgaaa
    14341 tagttaaaac cgttggtttc acaacctttt tcgcctaata acggactggc tttacaccct
    14401 tccggcacta ccggtaacga gccaaaccag gccgccacat cgccctgcac ttttggcgtt
    14461 aatgaccagt tcatccattt gtaggcgcaa accggatgtt tcgcttcgct atgcagcatg
    14521 gtggtatcag cccaaccggt aacaccctcc ttcgggaaaa cggtagcaac aggctggcct
    14581 tcggctttca gggcgttggc ctgatagggc caggcactgg aagcaaccac accctcgttt
    14641 ttgaaatcgc tcatttgcac ggtagtgtca tgccagtagc gatggatcaa actgtgttga
    14701 gcgcgcagca ctttcagcac cgcctggtac tgttcttcgg ttagttgata cggatcgctg
    14761 atgcccaact gcggctgagt ggctttaacg aacaacgcag cgtccgcaat gtagataggg
    14821 ccatcataag cctgaacgcg gcctttattg ctcttgccgt ccggcagatt ttgctcaaca
    14881 aaaaccactt gccagctatc cggcggcgtc gggaaggttt tagtgttgta catcagcagg
    14941 ttcggccccc attggtaagg tgtgccgtaa actttgccgc caacattaaa ccagtcgcct
    15001 ttaaccacgc gcggatcgag cgttttccag ttgggaatca aggcggtatt aatcggctgc
    15061 acgcgtttgc ccataatcaa acgcagcgag gcatcgccgg atgccgtaac cagatcgtaa
    15121 ccccctttgg tcatcagact gaccatttca tcggaagtcg cggcggtttt cacattcacc
    15181 gcgcagcctg tctctttttc aaattgcgtt acccagtcgt attgtttatc agtttgtccg
    15241 cgttcgatgt atcccggcca ggcgataata tccagtcgcc cttccggttt atctaaattg
    15301 gtaggtggtt cggcggcgtg agcgttcatt attgtcatgg tgagcgcaca caggctgctg
    15361 cgggcaaatg tcttgctcat aaggtcttac tcctgtcgta attaattgtt cagccgccgt
    15421 gccgtaaaaa tatctccttg tttaaccata gacggcggct tgcaggcaca ccttcgcccc
    15481 tgagaaaatt taatcaggtt gtgagctacc gcgcattatg ccgtctgtaa ggttatcttc
    15541 tggagtatag acaaggggtt aggaaatatg actgctattc gaaattcata ttacccaatc
    15601 taagaggaat taaattaaac gctattacag ccgttcttga ataagtttgc ctaattgttt
    15661 taccgcctgt tcttcacgtt ctccccactg ccacgcggta ttaaaacgaa aaaaacgtga
    15721 ccagttttca ccggtagaaa acattttacc cggcgcaata ctgatatgat gcgtcagtgc
    15781 cgccaggctt aattcgccgg catctaacgg ctcggggagc tccagccaga gaaaataacc
    15841 gctgtcacta tgatgaattt taacttccgc aggcagataa cgcagcagtg cctgccaggc
    15901 acgttgttta cgttccgcaa gctggcgacg caggcgacga agatgggcgt cgtatcggcg
    15961 cgtggaaagg taatccacca gcgcaagttg catcggtgag ctggtggaaa gcgtactcat
    16021 caactgcaag cgttgaattt tacgtgcatg ttttccggcg gcgacccaac caatacgaaa
    16081 accaggcacc agacatttcg aaaacgaaga gcaatgcaaa acgccatcgt ggcgatccca
    16141 cgctttcgca ggcagcggtt tttcccgtcc aaaataaagt tcgctgtaaa cgtcatcttc
    16201 aatcagcgtt acgttgtact gattgagtaa cgccaccagt tgtgcttttt tttgcggcgt
    16261 taaggtaaat ccgagtgggt tctggctatt agtcatcagc cagcacgctt tcaccggata
    16321 atcctgcaac gccagttcca gcgcctgaag atcgatccct tctttaacat ccgtcgccac
    16381 cgataacgcc ttcagccgta gccgctccag cgcctgcaac gcaccgtaga aacaaggatt
    16441 ctctactatc acccaatcgc ccggttcagt taccgcttgc aaactgaggt ttaatgcctc
    16501 taacgccccg gcagtaatga caatttcatc aggagaaatg gtgatgccct gtaaggcata
    16561 gcgacgagca atagcctgac gcagttctgc gtttcctggc ggtaagtttt caatcacgct
    16621 catcgctgtg gcggttttgc ttacctgcgc cagcgagcgg tttagttgtt ggagggggaa
    16681 aagtcgcggg tcgggaaagg ccgaggcaaa cggaacgacc gacggatcgc gactggcctg
    16741 caacatatca aaaatataag tgttgatatc gactgcttca tctcgagtga ctggaatgac
    16801 tggcgctttc ggcattttta ttgcctgtgg cgcaacgtaa taacccgact gcggtcgtgc
    16861 gataatatat ccctgacttt cgagcaactg ataggcatgg ctgacagtca taaagctcat
    16921 gcctgaaagc gccacctggt cacgcaacga aggcaaacga tcgccgggtt gccagatacc
    16981 cgacgcaatc tgctcgcgta attgttctgc aagctgctgg tattttttca ttatcgaatc
    17041 gtaattatgt gcagatgatt cggcagtcta tatcagttgt taaaaatgac aacttttcgt
    17101 taactgtaat taaaccatca ccagcgataa ctctttgcca agcgcctttg ccgcaagctg
    17161 gacggcgtcg atttttgtcg catggtgcaa gttaaatagg cgagtaatct cctgtttagg
    17221 tttgccaatt cgcctggcta actcttgctg agtgatttct gactgtaaaa aagcatttaa
    17281 cagcaatact ttagaggcga cgctcaaagg tacttcaata aagtgatcat gactatttaa
    17341 tggcgaaggt aacgggataa gctcgttatc ttcaaaataa aaatcaaatg cggtcagtaa
    17401 agcatctttt gccgcttcca tcgcttcagc gacagtttcg ccctgcgtca acgcttcagg
    17461 gatatccaca aaagaaacca tataaccgcc ttccggcgcg ggtgtaagag tgacgggata
    17521 acgcataatt tcgactgtct ctcgcatgtc attaaccttt ttaattggcg attaactcaa
    17581 accgagttgt ttcaggattg ctttacgcag gagtgacgcg gcatgacact gcgcctccca
    17641 tgaaacctga gtttcaaatg gttgcggcca ttcgctacat cgacgccctg agattcgagc
    17701 caacgtctga actcgctttg tttcacatcg cctccctgcc gctgagtata tcaacaaagt
    17761 aaacattagt gtttacctct gtgtgtaatt tgctcgaaaa tagcactaaa tcgccgcaac
    17821 ggcagaatgc aatgcaaaat caggaaggaa gattccagaa tttctttgcg ctaatttgca
    17881 aaactgaata ataacctcaa gaaacgatcg gcatcatagc ggcaaaaggt aagaaaaagg
    17941 tggcaggatg tttacgaagg cgttatcggt tgtcttatta acgtgtgcgc tgttctcagg
    18001 acaactcatg gcagggcaca aaggacatga atttgtgtgg gtaaagaatg tggatcatca
    18061 gctgcgtcat gaagcggaca gcgatgaatt gcgtgctgtg gcggaagagt cggcggaagg
    18121 tttgcgcgag catttttact ggcaaaaatc gcgcaaacca gaagcgggac aactttgagt
    18181 catttgttgc cggatgcggc gtgacgcctt acccggcagg caatatcatg cagattcaat
    18241 gagttgtgag taaccagaaa actggcgttt tacccttttc gcttaggcaa tgttttcagc
    18301 agctcatccg gacttacgga tgccactacg cttcccggta acggcatttt cagtatgtga
    18361 tttttgattt tgccaatgac gtgcatctcg catgggcggc aatcaaactt tagcgttaat
    18421 acttcatcgc cgttaaccag ttgcattggc gtcgccttcc agcttttgat attgcctttc
    18481 gcctgtttcg ggcacagatt aaaggcaaag cgcagacaat gcttggtgat catcaccggg
    18541 acttcgccct tctcttcatg tgcttcatac gccgcgtcaa tcagctgcac accatagcga
    18601 tgataaaatt cacgcgcttt ctggttgtat acgttcgcga ggaaactcag atgcgtttgc
    18661 ggataaaccg gcgcaggatc agcaaccggt ttacggctgc cgcgctggta actggcaaga
    18721 cgcgcagcat ccagcatgtc agcagcttca cggcggaact ggtttaacag actgtttggt
    18781 acaaacagcg cccccggcaa attaatttgc acatcgcggg cgtaatagag ggtttgcccc
    18841 agttttgcca gaccatcctt cagattgttc attgcttttt cggcgttatt ggcttcgtcg
    18901 aactgcccgt ccagcgtatg cgtgatgctg acaccctctt cactggtgag ggtcagaatc
    18961 agttgttcct gccagccgcc cagttcaatg tctaccgcca cccgacgttc gctggaggtt
    19021 tttgtcagtg cctgctgcca gttatgatca agattacggt ttagtgggtg atgtggacga
    19081 attttgtgca aatctgctgg catttcattg ggccagacgc ggtactgatt ttctccggtt
    19141 ttctcgaccg tattggcacg aaaaccgacg acttcacgtt taatcaacac gttcaggcca
    19201 tcgccatttg ccagtggctc ggtaacggca acatcgagat gatctttcgc cactttcaat
    19261 acttcgccta ccggcaggcc gataaatttc ggcgaatcga acgcgccaat atcgccttta
    19321 cgggcattca caaaataatc tgtgctacca cggtggaaag tcttttccgt cgatggaaca
    19381 aagaaatgtt cagtacgacc tgatgaagcg cgcgccagat cgccacgttc ttcaataatg
    19441 gcatcaagca tctggcgata atgggcggtg atattcttca cgtagctcat atctttgtaa
    19501 cgcccttcaa tcttgaagga gcgtacacca gcatcaatca gcgcgccgag gttggcagtc
    19561 tgatcgttat ctttcatcga cagcagatgt ttttcatagg aaaccacccg cccctgatcg
    19621 tctttcaatg tgtatggcaa acggcacgcc tgcgagcaat cgccacggtt ggcgctacgc
    19681 cctgtttgcg catgagaaat gtagcactga cccgaatagg ccacgcacag tgccccatga
    19741 ataaagaatt caatggtcgc gtccgtagcc tggtgaatcg cgcggatctg atcaagattc
    19801 agctctcgcg ccagcacaat ctgcgtgaag ccaacatcag agaggaactt cgctttttca
    19861 actgtacgaa tgtcgcactg cgtactggcg tgcagttcaa tcggcggaat atcaagttcc
    19921 agaatcccca tatcctgaac aatcagcgca tcgacaccgg tctggtagag gtcagtaatc
    19981 agccgttgcg cgggttccag ctcatcatca tgcaaaatgg tgttaagcgt gacgaaaatt
    20041 tttgcaccat aacgatgggc aaacggcacc agctcggcaa tatctttcag gctattactg
    20101 gcattatgac gggcaccaaa accagggccg ccgatataaa cagcatcggc accgtgcaaa
    20161 atagcttcgc gggcaatggc ggcatcgcgt gccgggctta acagttcaag tcgatgagaa
    20221 gatacggtca cattcttatc cagtagctga aaaatggcgg ctattttagc cacaagcgcg
    20281 acgttgcgaa atagttttcc cgcttaactg cgcgggtaat ggatgaggga atgaaaatgt
    20341 acggtttgct ccccaccatt acgatagata tgcgtgacgt ctgcagcaaa tcgaacgcct
    20401 tccccacaat taagggtctg ccattcccca tcaacgcaca gatcaagttg tccatcaatg
    20461 acgacgacat gttcgattac ccccttctca tggggcgtcg attcgcttat tgcaccgggt
    20521 gccatctgaa tcgagaagtg ttcaaagcag agttgtggat cataaggaaa cagcgaagtg
    20581 atcaccatcg cctgctgttg tggatcataa actgaaggcg tagcggactg tggcggagaa
    20641 ataaatgtcg aaaatggcac attcaacccg gtggcaattt tccataatgt cgcgaccgtc
    20701 gggctggact cattacgctc aatctgccct aacattgctt tagaaacgcc cgtcgcttct
    20761 gccagtcgcg aaagactcca gccgcgctgc tggcgtagtt gtttaagtgt ggtggataaa
    20821 aagcgagcga gattttccat gcgactcctc cggcaaaacg gaagtttatc acttgtgcgt
    20881 tataacggac aaatgctacg gtgcctgtac gctataacgt acgaggtgac tatgcgtctg
    20941 ttttctattc ctccacccac gctactggcg gggtttctgg cggtattaat tggctacgcc
    21001 agttcagggg caataatctg gcaagcagcg attgtcgccg gagccaccac tgcacaaatc
    21061 tctggctgga tgacggcgct ggggctggca atgggcgtca gtacgctgac tctgacatta
    21121 tggtatcgcg tacctgttct caccgcatgg tcaacgcctg gcgcggcttt gttggtcacc
    21181 ggattgcagg gactaacact taacgaagcc atcggcgttt ttattgtcac caacgcgtta
    21241 atagtcctct gcggcataac gggactcttt gctcgtctga tgcgcattat tccgcactcg
    21301 cttgcggcgg caatgcttgc cgggatttta ttacgctttg gtttacaggc gtttgccagt
    21361 ctggacggtc aatttacgtt gtgtggaagt atgttgctgg tatggctggc aaccaaggcc
    21421 gttgcgccgc gctatgcggt aattgccgcg atgattattg ggatcgtgat cgttatcgcg
    21481 caaggtgacg ttgtcacaac tgatgttgtc tttaaacccg ttctccccac ttatattacc
    21541 cctgattttt cgtttgctca cagcctgagc gttgcactcc ccctttttct ggtgacgatg
    21601 gcatcgcaaa acgcaccggg tatcgcagca atgaaagcag ccgggtattc ggctcctgtt
    21661 tcgccattaa ttgtatttac tggattgctg gcactggttt tttccccttt cggcgtttat
    21721 tccgtcggta ttgcggcaat caccgcggct atttgccaaa gcccggaagc gcatccggat
    21781 aaagatcaac gttggctggc agctgccgtt gcaggcattt tttatttgat cgcaggtctg
    21841 tttggtagtg ctattaccgg gatgatggct gccctgcccg taagttggat ccagatgctg
    21901 gcaggtctgg cgctgttaag taccattagc ggcagtttgt atcaggcgct gcataatgag
    21961 cgtgagcgag acgcggcggt ggtggcattt ctggtaacgg caagtggatt gacgctggtc
    22021 gggattggtt ctgcgttctg gggattaatt gccggaggcg tttgttacgt ggtgttgaat
    22081 ttaatcgctg acagaaaccg atattgacat cctccacgcc ctgaaggacg tggatccctg
    22141 ctacgttcag gctgtcgcct gaatcatttc ggtgggttcc tgcttcaacg ggcggcctga
    22201 ctgcaccatc tctccacagg caagaacggc gtgccccgcc gctaaaatgt tacgagcgcc
    22261 atttacatcg gcgttcgctg tatatccaca tacctggcat ctgaatttac tttgtgacag
    22321 gcggttctct ttcgctgtat gaccacagta cgcgcaacgc tggcttgtat acgctggcgg
    22381 aacagcaagc acctgaccgc cacgccagag ctgcttgtac tcaagctggc ggcgcatttc
    22441 ataccagccc tgatccagta tcgaacggtt taaacctgat tttgcccgga cattgcgccc
    22501 cggctggctg accgtacccg ctgctgactt tgacatgttg ctgaccttca aatcctcaat
    22561 gactatcatt gcgtggtttt tgctgacggc cgttgtgact ttgtgaaggt agtccctgcg
    22621 gatatttgcg atacaggaat gcagtcgctg tattttgcgt ttctgcttct gccagttgtt
    22681 gctgaatttg accttgcggc ttaactgtcg ctgaagtctc gccagcgtct tctggttttt
    22741 ctgaaaactg tttacaggct caaagactgt gccatctgac agcgtggcga gtttagccac
    22801 gccagcatcc agcccgacca ttgatgctga agggtgaacc ggagtggata cttcactttc
    22861 tgtctgaata ctgatgtacc acttaccgca ggactggctg acagtgacat ttttcacaac
    22921 acccgtgacc tgacggctgt tccggtagcg catccagccc agtttcggca gaaaaatacg
    22981 gctgttttcc tgatcgagct taacgccctg cgggtagcgg aatacatcat tctgtccccg
    23041 ctttttgaat cggggaaaag cagcccgatt ctggaagaag tttttgtaag cccgctcaag
    23101 gtctttcagt gactgttgca atggctgtga cggggcatct ttaagccatt gcgtttcagt
    23161 ggcatttttc cactcaacca gccaggaagc cattttcccg taagggatgt atttattacc
    23221 gacctcatga ttctcattct gacgtgccag agcacgattg aaaacgaaac gacatgcgcc
    23281 tgcgaagcgc ctcatttcac gctcctgttg accaccgggt cttaactgga atttaaatgc
    23341 ttgtagtcgt ttcacaggcc aaatgatact ttggtctgtg aaaaaagaaa ccgatatccg
    23401 gcgtggaagg cattgtgttt tcctgatgca tgttcacctg gtctttgtca ccagataccg
    23461 acgtcagatt tttgaccatg acgcgacaga aaaactacgc acttactttt caaatgtatg
    23521 tgcttatttt gaagctgaac tggttgaaat ggatggcgaa ccagatcacg tccatttgtt
    23581 aattaactaa cctcccaaac tggcgatatc cagtctggta aacagcctca aaggcgtatc
    23641 gggtaggtta ctgcgacgag atcgaccaga tattgcagtc aggtattact acaaaggcgt
    23701 tttgtggagt cctggctatt ttgccagtag ctgcggaggt gcgccaatat ccgtcatccg
    23761 ccaatacatt gaacaacagc aaacacctgg tcaggtggaa aaccgcgcct tatatccccg
    23821 ccctgaagga tggggtttta cggcgcaccg gataaaaagc gccatctaac gatggcgcgt
    23881 ctggaactac tttttgttaa cgtcaaacat ggtggcatcg gtcgccatgt catcaataac
    23941 ttgtttaata ttttcgaaag ccattggcgt actttcgtta ttcaggtctt taccttcgcc
    24001 ctgacgaacg actttgataa ccggtttatt agtcgctgca tcaatcagct caccttcaaa
    24061 atagaggcga gtatccatgg tacggtggcc tgtagccatt tgcgtccccg caaccactaa
    24121 tgcaacagga accacttcat agaattgcag cccttctttg ctggtatcta caccggtaat
    24181 ggccccacgg aaaatcagac tacgcggccc agcggtggta accactggtt tacgctgcgc
    24241 tatcgcttct ttcatttcgg tgttggtata gttcaaaatt ttatccagaa ctttctgccc
    24301 tacctgggta gaaggttttg gtaccggata ataagagatt gggttccaga cgatgctgtc
    24361 atatttgctt tgatcaaaac tcgggtctac ccaacgtaaa acaggtttac ctgttgctga
    24421 ggttgtttct tttaaatcag agtaattgtt taaaaaacca gaatatttat ctggctgggt
    24481 gattttagag gcacaaccag acagagccaa caagccgcat aaagctgcaa ctttcgcaaa
    24541 tgatgtggta cgcatgaata gtttcctgag atgattatat tactttctaa gttatagcaa
    24601 aaagcgccgc ttcgtgttgt ggcaaaaaaa gtgttggtga aaataaattt ttcagcccct
    24661 tttcagacga catagtcaat gccgaaaaag gggctaagtc aatcatcagt agttaaattt
    24721 cagtaagtta agctgctaaa aacttcgact actgtgatta atttcacatc ccttcgataa
    24781 aaaatatgct taccgaaaag aagaacagta aatcatctca gcgtggggaa ttttcacgcg
    24841 ttttgtgctt accgggtcat tttttacgtg ccagcatcgt ggcgaaacgc agtttaatac
    24901 gattaccgtt ggcgtcggtg cggtgcagct cgccgacgtc ttcattgtat ttcaccctct
    24961 cccagccttc ataatatcga cgtaattctc cctctttgaa ggcaaacggg aagccgacgg
    25021 tacatggata atcagcggta tccatcgccg ccacaatcag gttgtaacca ccaggtttag
    25081 tgcaacgttg catattggca atcagccctg ggatggtttt agcctcaagg aacatcagta
    25141 ccacagtcga aagaataaaa tcgtactgtc tatcaaatgt gaggttattc agatcaacga
    25201 cccgggtgtg taaattatcc agattttcaa tggatttaat gcgctctaca ttggcgatac
    25261 tcatagcatt tttatcccat gcgtcaacat cataaccatt ggctgccagg taaagactgt
    25321 tacgaccatt gccacagccc agatccagcg ttttacccgg tttaaccact ttcaccgctt
    25381 ccagtacttc agagtgtgtg cgggttaatt catatttatc agtaaaatag ttttcgtcac
    25441 gaatgatcat tctttgtcct ctgctttcat taaaacggcg cgctcggttc tgactaacaa
    25501 ttttccctgc atcagaagcg caaaagtacg gatgagcagt attgcaataa taaaattggt
    25561 aaagataaac agcggtaccg ccagcgtatg gaaaaatcca ttttcgctgc cactccccag
    25621 atgcaaaccg gtggttgcca gtgcagatac gccgaacgag aaactccaga atgaagcatt
    25681 aaatggctgg gagagatacc atggcatcag acgtagcata aacagcagtt gcagcagtcc
    25741 ataaccaaaa agcattttcg ccagcgtatc accctcgccg ccgttgacgc tcaaccaggc
    25801 actacaggcc accagcgcag gagcgagctg aatgccgagt gatgtccgca gtgccgtggg
    25861 taattctccc gaactgcgca gacgctgcaa gatcacaggt tcaaggctta gccatgagaa
    25921 aacgcctgcg cctaaaaaca ccagaccggc gtcggtgtag cccaacgcac cacaggccat
    25981 tgcgctgata aagttgttgg caactgtcgg cagatacagt ccaggcgtgg tagcttcttc
    26041 agggtgagat ccgcgccata atcccgcagt ttgccaggcg gcataagcca actgaaccac
    26101 gacaccaaaa ctgaacaggc acaccgccag tgggcgaaac cacggaacaa aaccaatcgc
    26161 caccagcatc gtcgttgccg gaaacaaact cacaaaactg ctcatcactg gatggcgcac
    26221 ttctgccagc acgctatgcg gaaagcgtat aagtcgggta ataaatgcgc tagttaacaa
    26281 tccccagatg atcatcgcca gaatcaccag cccatcccct aaccagtggc tgacctgcca
    26341 aacctggctg gcatagcgcc aggcaaatcc catccctatc gtccccaaca caataccaaa
    26401 gtagcctgcc ggcaaattga gcactttatc gctctgcatc ttcttactca ttttgtttaa
    26461 tttataaagt atatttgaaa tgcattttat ggaattttct cgagcatagc cagagccgca
    26521 gaatttgcta cggttatagt aagtcacctg tgggaaaacg ctatgcgcaa atatcgtcta
    26581 agtgaagaac agcgagcctt tagttatcag gaagatggca ctaaaaaaaa tgtgttatta
    26641 cggcagatta tcgccattag cgattttaac gatgtgatag caggaaccgc tggcggctgg
    26701 atcgatcgcg aaacggtgtt agcacaggaa ggaaattgct ggatttacga ccagaacgct
    26761 attgcgttcg gcggagcggt gatttccggt aatacgcgca tcaccggcac cagcgtgctg
    26821 tggggagagg tttacgcaac ggataatgtc tggatcgata atagtgaaat cagccagggt
    26881 gcatatataa gtgacagcgt caccattcat gactcactgg tttgcgggca atgtagaata
    26941 tttggtcacg ccttaattga tcaacattct atgatcgtcg ccgcacaagg cttaacgccc
    27001 gaccatcaac tcctgctcca aatttatgat cgcgcaaggg ttagcgcctc gcgtattgtc
    27061 caccaggcac aaatttatgg cgatgccgtc gtccggtatg cttttattga acatcgcgcc
    27121 gaagtttttg attttgccag tgttgaaggt aatgaagaga acaacgtctg gctatgcgat
    27181 tgcgctaaag tctacggtca cgcgcaggtg aaagctggca tagaagaaga tgccattccg
    27241 acgattcatt acagttcgca ggtggcggaa tatgccattg tggaaggtaa ttgtgtgtta
    27301 aaacatcatg tcttgattgg cggaaatgcc gtggtacgtg gtggaccgat tctgctcgac
    27361 gagcacgttg taatacaagg tgagagccgt atttcaggtg cggtgataat tgaaaaccat
    27421 gtggagttaa ccgaccacgc cgtcgttgaa gcctttgatg gcgacacggt tcatgtccgt
    27481 ggtccaaaag tcattaacgg cgaggagcgc attacgcgaa cgccgctggc agggttatta
    27541 tgaatcgata atacgcgcgt ataagttcac atcatcatag gcatcattca ggaactcagc
    27601 ctgtttcagg caaccttcaa ggataaaacc attgcgcaaa gcgacctggt tgctttgcgg
    27661 attgtccacc cgacatttga tcacgaagcg tctaagttca ccagactgag cgtaatgatg
    27721 aatcaatgcc tgcagcgcct gagaaatgat cccctgcccc tgatgagatt cgtccagcca
    27781 gtagccgatt tcagcagttt tactcagtgg ttcaatacga ttaaacgaga taacgccgat
    27841 aagttcatct tctttgaaaa tcatgaacat tttggcatag ccgcgttgat gcaacatcac
    27901 attaccctgc accgtttttc gcgtgtcctc ttcactctga acgaactgcg gccagtttag
    27961 cgactgctgt aaccaggttt tatttttaca gattaactga taaagaggtg tgacgtgact
    28021 ttccgcaaca gcatgtaatt caagtgattc gcttactttt atcgtttcag tcatcttatc
    28081 tccagtcaat ccatgctgtt gttaaaaaag ccgcctggct ggcggcttag gaaagtttac
    28141 acctctttga cgctctcctg ctgcaggatt ttgagcatct catcttttaa ctgtagcttt
    28201 tgttttttca tgcggaccac ttccgcattg taccctcgac cgtcggaacc ttcctttctg
    28261 gcaatttcat gatcaagttt attgtgttta tcgaacaagg acataaagcg aggattttcg
    28321 tttttcagac gggatattaa atctcggtat tctggaaaca tacgcactcc ctgttagtgg
    28381 ctgacaaggt gtatctgaaa tacacaccat cagattactc atttaactta gggaaaaatg
    28441 cgagcgggat cgtgtttaac cagacggcac ctcccgaaga aggtgccgaa gaaaaaacga
    28501 ttaactcatc acgcggtcgt caacatactg acgtttatcc ggcgctggcg ggaaatactg
    28561 atacagccat gtttcactga tagcgtcgcc ctgacaacgc agatacatcc gcatatcgac
    28621 cggatcagtg gagtccgaag tcggatacca gtcaaactga atacgataac catcaatggg
    28681 ttcaatatag agaatttcga tttgcttcgc ttccccactg gaaagcgtaa tcaccggctc
    28741 aatgcctttt ggtgcggcag ctttcagatc accaccaacg aaatcgacgg caaaacgacg
    28801 cgcccatttt tcgggatagt gttcacctgg tgcccaacct tccgggaaac cgcccatgcc
    28861 ggtacgcgtc gccataacgc gcgctaatgg gcaatgaaca ggcggttgcg cactccagta
    28921 cagacgatac tggaatgcaa actcatcacc cgcctttaca gctttttctg gctgccagaa
    28981 gcagacaatg ttatccagcg tttcgcccgt tgttgggatt tccatcaggc cgatggtgcc
    29041 cttaccccac ttgttacgcg gttccaccca cagacttggg cgtttgttat accagcccat
    29101 aatgtcctga taatgggaga aatcacgatc cagttgcaat aaaccaaacc ctttcgggtt
    29161 gttgtcggtg taagcattga actgcaattt ttgcggatta ttcagcggac ggcaaatcca
    29221 ctcgccgttg ccccgccaca tggacagacg atcagagtca tgaatttgcg gatgaatggt
    29281 gtcgcacatc cgacgttcat tagtaccgca gctgaacata ctggtcatcg gcgcaatgcc
    29341 cagctgttta atgtctttgc gcgcatacag gtgattttcc acatccataa tcacctgact
    29401 tttctcacaa tggatagtga acttataggc accagtaatg ctggcgctat cgagcaacgc
    29461 ataaacggta aaggtagttg cccccggttt taccgtatca aaccagaagg cggtaaagtc
    29521 ggggaactct tctttactgt cggtgtaagt gtcaatcgcc aggccgcggg ccgacaaacc
    29581 gtattgatat gtgtcatcaa cggcgcggaa ataactcgct ccgaggaatg atactacatc
    29641 acggcgcgcc agttcggggg ctttaaacac gcgaaaaccg gcaaagccga gatcgctttg
    29701 cccttctaat tgtttggtat caacacctgc atcgttgtat ttgaacaact ccgggcgaaa
    29761 gtgaatttca cgcgccagat gtgttgctgg atctacagaa aacatacgaa cgcggcgacg
    29821 gaatcccatt cccatatgga agaactgagc gtccagttga cggttctcaa cgttatgcca
    29881 gagcgatttt tcggcgtcgt attgaatact gttataagcc tgcggcgtca ttgtcgccag
    29941 cgtgtcaggt aacggacgag gcgcaccacg ccacgctgtt tgcgctaagt cgtgcgccat
    30001 tgactgtaga atggagaagt caaaacgctg ggtttgcccg tcggcaatat cagaatctgc
    30061 cgcgaatgcc gcctgagaaa aaagagaagc aatgccgctg gtaccgcaca cggcggccat
    30121 agccattgaa cctttaataa atcgtctacg atccatacct gaaagtgagt ccttctggtg
    30181 ttgtgaatgg tcccgcgctg gtcgttttca gcggcaagaa agtgcttatt tatgaccgct
    30241 cactctagac aatatttatt aataatccaa ttgttgaact cagagaatat gcataaatcg
    30301 gcaatttttt tatgtcgatg ggatgagcct gattcgtttg taaaatcagg caaatttttg
    30361 caaaggcgga tggtcagaat ttaaagcaaa ccacagcgac gtttactgcg ttcttccgct
    30421 tcctgataaa gctggaactc atccaaaacc ggacaaccca acgcctgctc aaatgcctcg
    30481 cgactggcat taccgtgatt gcgtgcctgc gtttcattac ccagatttga ctggaagatc
    30541 cccgccgcgc taacgggcaa gaaatcttca taggtaatgg gttgcgccgc gacccaacca
    30601 cgttcaatta agggctgtgg atcgtctccg ggatgaatcg cctgacgatg cgcctcacct
    30661 gaaggcgtca gacggtaccg gaaccatgcc agcccttgct gacgcattaa aaactcactg
    30721 tcaggaaaag tgcggaaggt ttcctgtaaa tgcatttggt gagtgagatt atcctgcccg
    30781 gttccagcgt tacgcagaag atcatcatac agttgtcgcc ctttcggcgt taatgccacg
    30841 ccacgctgct caatttcacc aaagcgcgcg gtatgcgtgc cctgtttctg ccccgcaaac
    30901 aacaccgtct cttccagtgc tttaaagctg gtctggcgta gtaaaatcgg tacctcgcgg
    30961 cgcggcggcc cctcgatgag aattttgggt tcaattccgc attcaggcat catcgactgc
    31021 acccggtcaa tatccagcgt acgtggcgtc aggtggttga tatggcatcc aggaaaacag
    31081 accacatcag caatcaaccg atgttcgttg tgcaaggcgc gataggtttc ttcatctacc
    31141 gttgctgact ggtgccagcg aaacgtttcc agggcttcct gcacaaactc ctgtgcctgt
    31201 gtttcgttaa aaccgccctg ctgctcatat tcctctaaca gttgtcgaca acgtggggtg
    31261 aagatatcgc gctgacgcag aatctccgcc gctttctggc gcaaaatttc gttctcgata
    31321 agctcaaggc ggagtaagga ggtaaaaacg cggaagggat tacgcgccag agaagcatca
    31381 tcaatgggcc gaaatgctgt cgaatgtacc ggcacccctg cctgcgagag atcgtaatag
    31441 ctcaccgggt acatccccat aatggcaaac atccgccgaa gagtagcaag ctcttgtgca
    31501 gtcccaacgc gaatcgcccc atgacgttca acattcagtc gcgccagctc gtctgcattt
    31561 accatttttt cgtgcagttg aggattgttt tccagcacag ccagattcac atcagctacc
    31621 agttccagca gcgtgccata ttgcggaact tcttgctggt acatggctga cattgcctgc
    31681 gaaaactgtt cccgaatctc atccgccgtg atgctgttcg ccatagtgtc tgcctccagt
    31741 ggtcagtaat ctggagtgta ggtaaccgca ttcactctgg cgggaagaat ttacaaactg
    31801 tgatctcgcc gcgaaaacat caatattatc cattttgctg taacataatt gctttaattg
    31861 ttaataatat tttgcaatca agttatcata atcaaacaac ttcactcgtc agcgacaccg
    31921 cttcgttttt aacatcgctt atggaaaaaa atagtctgtt tagtcagcgc atccgtttgc
    31981 gccaccttca tacattcgta gctgtcgcac aacaaggaac tttggggcgc gcggctgaaa
    32041 cccttaattt gagtcaacct gcgctctcta agacattgaa tgaactggag cagctgactg
    32101 gcgctcgctt gtttgagcgt ggtcgtcagg gggcgcaact taccttaccc ggcgaacaat
    32161 ttttaacgca tgcagtcaga gttcttgacg ccatcaacac tgccggacag tcgcttcatc
    32221 gtaaagaaga tcttaataat gatgtcgtca gggttggtgc actacctact gcggcactgg
    32281 ggatattacc ttcggttata ggtcagtttc atcagcaaca aaaagagacg accttgcaag
    32341 ttgcgacaat gagtaaccct atgattctgg cgggtttaaa aaccggggaa atcgatatcg
    32401 gcattggtcg gatgtcagat cctgaactga tgaccgggct taattacgaa ctgctgtttc
    32461 ttgaatcgct gaagctggtt gtccgcccta atcacccgct acttcaggag aacgtaacgc
    32521 taagccgggt gctggaatgg ccggtcgttg tatcaccaga aggcactgcg ccacgccagc
    32581 attcagatgc attagtgcag agccagggct gtaaaattcc ttcgggttgt atcgaaacgc
    32641 tgtctgcttc gctatctcgt caacttacgg ttgaatacga ctacgtgtgg tttgtccctt
    32701 ctggcgcggt aaaagacgac ctgcgtcatg ccacgctggt ggccctgcct gttccgggac
    32761 atggtgcagg cgaaccgatt ggaatactga cccgcgtaga tgcgacgttc tcttctggtt
    32821 gccagttgat gattaacgct attcgaaaat caatgccgtt ctgaaaggtg aagggatctg
    32881 tcgatccctc cttgaacatt ttcacaccgt agcgaaacta actggttcac ccgctccgcg
    32941 aggttctgcc gacacagaat gtttgttcag acggaatacg tccaccgcct cagttaatcg
    33001 tgccgcctgt tcttcaaggg acaccgccgc tgctgaggcc tcttctacca gagaagcatt
    33061 ctgttgcgtc accttatcca tttcagaaat cgcctggcta acctgagtta tgcctctact
    33121 ttgttcatcc gaggcggcgg cgatttcctg catgatatgt gtgacactcg cgacggcatc
    33181 aacaatagtg ctcatggttt tccctgccgt tgccacctca tccgacccca ggtcaattaa
    33241 cctgactgat tcactgatca agccttcaat ctctttcgcc gcttgggcgc tgcggcttgc
    33301 gagtgtccgt acttcgctgg cgacaacggc aaatccacgg ccttgctcac ccgctcgcgc
    33361 ggcttcaacg gcagcattca gtgccagaat attcgtctgg aaagcaatac tgttgatgac
    33421 ggcggtgatc tcggaaattt tcttcgaact tgtagagata gcgcccatcg tttttactac
    33481 accggaaacc gtctgcccgc catcgctggc tttaatagaa gcctcttgcg ccagtttgct
    33541 ggcatgatgc gcgttatccg cattctgttt caccgtcgca gtgagttgct ccatgctggc
    33601 ggcagtttgt tcgatagccg ccgcttgttc ttcggtgcga gatgacaggt ccgcattgcc
    33661 agctgaaatt tcgctggtgc cacgataaat ctcttccgcg ccctgtcgaa cagtccctac
    33721 tgtcatcccc agtgaatgct gcatttgctg taaatgacga cttaagcgac cgatttcatt
    33781 acgacccgca ggttcatcat tcatcgtcag atcgccactg gcgatttttt caatccgttg
    33841 tgcggcatgt tgcagtggac gaatgacgat ccgacgtagc accataaatg ttatcagcgt
    33901 catgaccagg gcaagcacaa acgcgccaat catgaacatc ccacccaggc gggtgcgctg
    33961 atgggccagt tccgccagtt ggttggctct ggtgctacgt atcttaacgg ctttgttcaa
    34021 aatatcggta taagcattat ccagcggtcg gatctgctca ctttcatgat tgataatcgc
    34081 ttcaaacatg ccatttttgg catatttcaa cataggttgc atacccgtga tataagcctg
    34141 aaagcgttga tttaattcag tgtcgagggc ttcatcagca ggtgttttca ccggccgatt
    34201 ctgataagca cgataacctt gctgcgactg tttaatctcc gattcggctt gcgcaatatt
    34261 gcgcttcatt gcttccattt ccgcaatacg actcgcagcc ccggcctgaa tcatattgat
    34321 ccgggctgaa cgcaaaaaat ctgaactgtt cgctaacccg gtccgaatct caatctccgc
    34381 agtaacatca cgctgatcgc gatcggcctg ccagaggaaa tagccagcca gagcagaact
    34441 taatgcgaat agaactaaga taccgccaag aatgcaggca aataacggaa ccagcctgat
    34501 gtgatgcaaa aaacctaatc gatgtgaggg agttgtattc attaccgttg tctctcgtcc
    34561 aggtttactc ctgatagaaa gtcatcggca cttgacggta attacttatg accaaaaacc
    34621 tctttgcgtc acatttttac aacaattatt gcagagaaaa ttaatgctat tggaatgaag
    34681 agagtaacga atggctaaac tggctaaccc gaatgacgaa aaattctgga aaacagcacg
    34741 caatttcgtg aagtgattat tttcgtccct aaggaaacct cataggatgc ctgcgggtgc
    34801 ctgagacttt ctgttttcag gctaaccggg aatcagaaag acgaaggccc cgaccgatat
    34861 ttctatcaac cgaggccaac gaatgaacct agcaattcag atcttagcct cttacccgcc
    34921 atccggcaag gagaaaggct atgaagcaca accctctggt ggtgtgtctg ctcattatct
    34981 gcattacgat tctgacattc acactcctga cccgacaaac gctctacgaa ctgcggttcc
    35041 gggacggtga taaggaggtt gctgcgctca tggcctgcac gtccaggtaa gggcaagcgc
    35101 ggggattttc cccgcgcatc tgactagcag attcattttc tcatggcacc ccttttacac
    35161 ttcagttagt gcatgttttt ttcgaggcac taatagaacc atcattacag acaaatttgc
    35221 cgcctgccgt acagtgtgag atctcccctt tcgaaccaga acagggtttg cgcccggcat
    35281 cggcataaaa ggaaacaagc gttcccataa acaaaataag tgccagtttt ttcatataac
    35341 ctccatggtt tatcgaatct tatgttcgat ttgaacgtta tacgctggtt tattttgata
    35401 ccgttgatga gatcaaagat cgggtgaatt gattaaaaat aagccagatg gacgtatggg
    35461 caaggattat ggttttattt gtcatacaaa taaatataat aggcgcttcc gatgtagacc
    35521 cgtattcttc gcctgtacca cgggtcggtt ttagtacagg cgttttcttt aaatatcctt
    35581 caggaacgtt tacgcggcat catgcgtaga agtgtgttgt ccttccagaa atagtggtgt
    35641 gccagtgcag ccgcagcgtg caacccgatg acaaaatatc ccagattcgc cagcgtctca
    35701 tgccacgact ttaagctatc aacccgttcg aaattggcct ctgaagcgta aggcatcgtc
    35761 aaaccaaacg caaaccacgg gttgccccgg ttatacatca tcaccaaacc aatcactggc
    35821 agcgcaataa acaggagata aatcaccaaa tgtcccaaat gcgccagtcc cgtcatcatc
    35881 ggttttggct taggtataat cggcggggtt gggtatttca gtcttaacaa aagacgaacc
    35941 accatcagca cgagaattga gataccacag gaaacatgaa ccatgttgat gagtggccga
    36001 tcgctacgtg ggaagaaacc acgaaactcc attgcgcaat acgctgcgat aaccagcaaa
    36061 aagaccagcc agtgaatgct gatttgtaac cttgagtatt tattttccat aacatttcct
    36121 gctttaacat aattttccgt taacataacg ggcttttctc aaaatttcat taaatattgt
    36181 tcacccgttt tcagtacgtt acgtttcact gtaagaatgg agattgccca tcccacaatc
    36241 ctggtctaag cctggaaagg atcaattttc atccgaatgt tcctgacagg agaaaaccat
    36301 gagtaaagtt ggtattaacg gttttggtcg tatcggtcga ctggtgttgc gtcgattact
    36361 tgaagtcaaa agcaacatag acgttgtcgc tattaatgat ctcacttccc caaaaattct
    36421 cgcctacctg ctgaaacatg attcaaacta cggaccattc ccctggagcg ttgattttac
    36481 ggaagattca cttatcgttg atgggaaaag tatcgcggtt tacgccgaaa aagaggctaa
    36541 aaatattccg tggaaagcga aaggtgcaga aatcattgtc gaatgtactg gcttttatac
    36601 ctccgccgag aaatcgcagg cgcatcttga tgctggtgcg aagaaggtgt tgatttccgc
    36661 ccctgccggt gaaatgaaaa ctatcgttta taacgtcaat gacaacactc tggatggcaa
    36721 cgacaccatt gtttccgtgg cgtcatgcac cactaactgt cttgcgccga tggccaaagc
    36781 cttgcatgac agtttcggga tagaagtcgg cacgatgaca accattcatg cctatactgg
    36841 cacccagtca ctggtggatg gcccacgtgg taaagattta cgtgcttcac gcgcagcggc
    36901 agaaaatatc attccccaca ctacgggggc ggcaaaagcc attggtctgg tgatcccgga
    36961 actgagcggc aaactgaaag gtcatgcgca acgcgtgccg gtgaaaacag gttcggtcac
    37021 tgagctggtg tccattctcg gaaaaaaagt gactgccgaa gaggtgaata acgcacttaa
    37081 acaagcaacc accaataacg agtcatttgg ttataccgat gaagaaatag tctcttccga
    37141 tatcattggc agccatttcg gttcggtgtt tgatgccacg caaacggaaa ttaccgccgt
    37201 gggcgattta caactggtga aaacggtcgc ctggtacgat aacgaatatg gcttcgtcac
    37261 gcagcttatt cgcaccctcg aaaaattcgc taaactctga cgcgcacagg cggaggaaaa
    37321 aacctccgcc tcttttactc attacgactg taaataaacc acctgggtct gcagatattc
    37381 atgcaggcca tgtttaccat ctgcgccgcc aataccggat ttacgccatc cggcgtggaa
    37441 gccttgcata gcttcgaagt tttcacggtt gatgtaagtt tcaccaaact tcagcccttt
    37501 aatggctttc atcgcgacgt tcagattttg ggtatagatt gatgaggtca ggccgtaatc
    37561 actgtcatta gccattgaga tagcatcttc cagcgtgtca aatgcgacca ccggcagaac
    37621 cgggccaaag gtttcctcat gcataatcga catttcctgg cgaacatcca gcagcaatgt
    37681 cggcggataa taatatcctt tcccgtctac tgctttgcca cccaacgcca ctctcgcccc
    37741 ttcttctact gcgcgcgcca ctttttgctc gaccctttcc agcgccgcgg cgttaatcaa
    37801 cggccccatc gcaatgtcgt tgcgttcagc ggggttacca aattgaaccg cctgcatcgc
    37861 ttcacccagc cgattgacga actgatcata aatgcctttc tgtacataaa cacgttctgc
    37921 acagttacac acttgcccac tattaatgac gcgtgaatca acgatggctt tgactgccag
    37981 ttcaagatcg gcatcgtcca ttacgatagc tggtgcttta ccacccaatt ccaggcacac
    38041 tttggtgatg tttttcgccg cagtcgccat aatcttctca cctgcagaga cgctgcctgt
    38101 catactgacc attgcgacct ttgggttacc cgccagttct tgcccaacgg tttcaccacg
    38161 ccccagtaca aggttaaaca cgccgcgcgg aaggcctatt tcatcgacga ttttggcgaa
    38221 tgcaatcgca ttgtttggcg taaattcact gggtttaatg acgatggtat taccggtcaa
    38281 aagagcggga gccattttgc gggcaatgag gaagaacggg aagttccacg gcagaatgcc
    38341 ggtagtcaca ccaagcgcac gtttaaacaa aagaatattt tctcctggac gatcgctttg
    38401 aataatctcg ccctcgtaac gccgtgccca ctccgccatg taatcgatat agtcggcagt
    38461 aaaagccact tcgacttcag ccagctgctg gatcttgccc ccttcttcaa caatcagcgc
    38521 actgatttca ctggcgcgtt cgcggatgcc ggcggagatt ttgcgcaacc aactggcgcg
    38581 ttcaatagca ggcaacgctt cccattctgg ttgtgcacgt tctgctgcat cgattgcctt
    38641 acgggcatcc tcggcctgac catcgggtat gcgggaaatg acagcctctg tagcagggtt
    38701 taccacatca atccatgcgt ctccacgcca ggtaacaaac tgtccatcga tatacatagg
    38761 atgttgaacg ggtactgaca tgggcgactc ctgtgattta tatgtttgtt ttcggtgaat
    38821 gcattgttaa ttaacatcaa tcatcagaga gacggcatcg tgaaaggaca attctgtgaa
    38881 gggcttcata aaaacaagtg taaaaggaat aaaaggcaga aataaaagtc aggagtcatg
    38941 cccgtttgca aacaagtggt tcacaaacgg gcaattttta acgtaacgag cgactttcca
    39001 tcgcctcgat gagcaccgca tcatgtttca gcgtttgcca tgcatggatg acttctgccg
    39061 gaaaatcaac gtgaacgata aaatctcgcc cgcggggacc gtagccatcg ctatcatcgc
    39121 gtaaacgcgg cagctcgcca gtgagtagtg agagataacg ctcaaccggc cataatcctt
    39181 gtaactgatt aataaagatt acactgtctg cgttatttcc taactgagga acgaatccgg
    39241 gataacttaa ccagcgtggt gcatcgggat tgtccccagt catacggcgg aacgtcgcca
    39301 tcgtgcgccg ctgcatggtg gggtcctgaa caacgatagc cgtatgaact cgttctacgg
    39361 cctgattcaa tagcgcgatg ctaaagcgtg cgttttcacc gcagtttgtt gactggtctt
    39421 caatccagat tttttcatgc ggaatgtgcc agaactgatg agcgatatcc gccaggatgg
    39481 tcgcttctgc tctgccagtg gtgcggatag tgttgtagtg cggatgctgt gcgatggcgc
    39541 tatacaaaaa agttgtcgag tgaccgatac caccactaat tagtaaagga atttgctgat
    39601 cgcgagcaat cttacatgcc gcgtcgatag tcggcataac cgcattgcct gcaaggatca
    39661 cgcaatcggc ctgatacggc acctcaccgg agaaatcatc ctgcgccagc cactgtccga
    39721 taacatttat ggcatctata gttgccggcg aaagcgtcgg aaacggggta atgttcatgg
    39781 ttcctcctta tcgttccctt aagaatactg cgtgagataa tagtgagaag cggagcactc
    39841 tgaaaggagg aagtcgtatg tccttaaccg ccactccgca agcctgacat atcttttaag
    39901 gaaatgctgt agacaaacat tattcatctt gcagcagaga attgtatttt tctgaattcc
    39961 tgaattttta cccttaccgt tttagaatta ataaaaaacc cggcgctaaa tgcttaccgg
    40021 gttttttctt tatcaggcaa atagcagggt taaccgctaa tctgctccat cgcctgcaaa
    40081 atacgcttat ctgaaatcgg ataaggcgta ccaagttgtt gagcgaagta actaacgcgc
    40141 aactcttcta tcatccaacg gatctctttc acggcttcat cctcacgacg tgcgggcggc
    40201 agtttgttga tccattgctg ccacgcctgc tggacgtttt cgactttcag catctgagca
    40261 cggtcgcgat gtggatcaac cgccagtttt tccagccgtt tttcaatcgc ctgcaaatat
    40321 cgcagcgtgt cgcccagccg tttgaagccg ttaccagtga caaaaccgcg atataccaac
    40381 ccgcccatct gcgctttaat gtcagaaagc cccagtgcca tggtcatatc cacccgccct
    40441 ttcagacgtt tgttgatatt gaacaccgca gtgaggattt gctcgacctg cttcgcaata
    40501 tccaccaccg tgtcattcag ttcggcgcgc actttttcat gcagcgcagc aaagccttct
    40561 tccgtccaga ccgggccacc attggcgtcg atcaattgat ccacaccgca ggagatacag
    40621 tcgtcgatca gctccagcac tttgccatac gggttaaagt acagtcccag cttggctttg
    40681 ttcggtaact tttcatgtaa atatttgatt ggcgatggaa tattcagcag cagtagacgg
    40741 cgaagaccgt tccacattgc ctgcttttgc tccagcgggt tatcaaacag tttgatcgcc
    40801 acactgtcgc gctcatccac cagcgccggc cacgccttca ctttgtagtt gccacgcttc
    40861 tgttcgtagc tttccggcag ctgaccaaaa ctccagatat gtaagccgct ctgctcgata
    40921 ccgtcatccg ccaccgcaga tagcgtttcc tgcactttgc ctttcagcgc atctttcaga
    40981 tcttgtagcg agcgcccttc ttttagcttc ttgtttttgt catccaccac gcggaaggta
    41041 attttcaggt gatcaggcac ctgatcccag tgccagtctt cgcggtcaac ggtaacgccg
    41101 gtcatccgcc gtaactcgcg ctcaaggctg tcgagcaacg gtaactccag cggtttgacg
    41161 cggcctaaaa acgcttcggc atagtttggc gcgggtacaa aattacggcg taccggtttc
    41221 ggcaacgatt taatcagagc aatcaccagt tcgcggcgca gaccggggat ctgccattca
    41281 aacccgcttt cctcaacctg gttaagtaac ggcagcggaa tatgtacggt cacaccgtca
    41341 gcatccgcgc cgggctcaaa ctgatagctc aaacgcagct tgagattgcc ctgatgccag
    41401 aagttcgggt aatccagctt gctgattttt tctgcgccct ctttgatcaa catgcttttt
    41461 tcaaagttga gcaaatcagg ggtttcgcgg ctgacttttt tccaccagct gtcgaagtga
    41521 cgagcggaga ttacatcgtg gctgatgcgc tggtcgtaga actcaaacaa cgtttcgtca
    41581 tcaaccagaa tatcgcggcg acgtgatttg tgttccagtt cttccacttc agcccgcagt
    41641 ttcaggtttt cacggaagaa tgcgtgacgc gtctgccagt caccttccac cagcgcgtgg
    41701 cgaataaaga gttcacgaca taacgccgga tcgatctggc tgtagttgac cttgcgcgcg
    41761 gcaacaatcg gcaacccgta aacggtgact ttttccgttg ccatcaccgc gccctgcgcc
    41821 cgttcccagt gcggttcgct gtaggtgcgt ttaatcaaat gctgagcaac tggctccacc
    41881 cattctggat cgatgcgcgc cgcaatgcgc ccccacaggc ggctggtttc taccagttcc
    41941 gccaccatta cccatttcgg cggctttttg aataaaccag aaccggggaa gatggagaaa
    42001 cgcgcgttac gtgcaccggt atattcctgt ttatcggcat ctttcatgcc gatatgggaa
    42061 agcaaaccgg tcagtaaggc gatgtgaatt tcgcgatact ccgccggttc gctgttgacc
    42121 ggaatgccaa gttctttcac cacctggcgc aactgggtgt agatatcctg ccattcgcgc
    42181 acgcgcagat agttgagata atcggtacga cacagtcgac ggaaggcgtt agaagaaagc
    42241 gccttttgct gctcgccaag ataattccac agattcacaa acgcgaggaa gtcagactct
    42301 ttgtcgtgga agcgacgatg tttttcgtcc gatgcctgct gcttgtccat cggacgctcg
    42361 cgcggatcct gaatggagag cgcggacgtg ataatcatcg cctcacgcac gcagccatgt
    42421 ttttgcgctt ccagcaccat acgcgccaga cgtgggtcga caggcaactg cgagagctgg
    42481 cgaccgagcg gcgtcagttt ataggcgctg gcctgttcat ccgtggtgat cgcgcccagt
    42541 tcttcgagca gacgcacgcc atcctggata ttgcgtttat ccggtgcttc gacaaacggg
    42601 aacgcagcga tatcgcccag ccccagcgcg gtcatctgca aaataaccga ggccaggttg
    42661 gtacgcagaa tctccggatc ggtaaactcc gggcgcgaga ggaaatcgtc ttcggaatag
    42721 agacgaatac agatcccttc ggacacacga ccacagcggc ctttacgctg attggctgaa
    42781 gcctgtgaaa tcggctcaat cggcaaacgc tgcactttgg tgcgatagct gtagcggctg
    42841 atacgcgctg taccggggtc gatgacgtat ttaatccccg gtacggtcag cgacgtttcc
    42901 gcgacgttgg tcgccagcac aatgcgccgt ccgctgtgcg actggaacac gcggttctgt
    42961 tcgctgttcg aaagccgcgc ataaagcggc aagatttcgg tatggcgtaa gttcagcttg
    43021 ttcagcgcat cggcggtatc gcgaatttcc cgctcgccgc tcataaagat cagaatgtcg
    43081 ccggggcttt cctggcttag ttcgtctacg gcgtcaaaaa tcgcctgcaa ctgatcgcgc
    43141 tcggtgtcat cggcttcttc aacaatcggg cgatagcgca cttccaccgg ataggtccga
    43201 ccggagactt caataatcgg cgcattatta aagtggcgcg aaaagcgttc cgggtcgata
    43261 gtcgcggaag tgataatgat ttttaggtca ggacgccgcg gcagcaactc tttcaaatag
    43321 ccgagcagaa aatcgatatt caggctgcgt tcgtgcgctt cgtcaataat gatggtgtcg
    43381 tactgcatca gcaggcggtc ttgctggatc tccgccagca ggatcccgtc ggtcatcagc
    43441 ttgaccatcg tgttatcact tacgtgatcg ctgaaacgca ctttgtaacc gatgcaaccg
    43501 cccggctccg ttttcagctc ttccgcaata cggttcgcca ccgttcttgc cgccaggcga
    43561 cgcggctggg tatggccgat cagcccttta atcccgcgcc ccagctccat acagattttc
    43621 ggtaactgag tcgttttacc agaacccgtt tccccggcga cgatcaccac ctggtgatca
    43681 cgaatcgctt cgagaatgtc ctgttttttc tgactaaccg gtaaattgtc aggataagta
    43741 atttccggtc gtgccgcttc acgcagcagg actttccctg ccgcctggtc aatctctttc
    43801 gccatctcct ggaaaatggc ctgttgtgca tcaggatttt taaccttctt cacaccgtgc
    43861 agacggcgag aaaaacgcag tctgtcgcgc agcatcagcg aatccagccg ctgctgcaag
    43921 gccgtaaagg tcaatttttg ttgttctgtc ataacgttag tgggcagtaa tctgcctgat
    43981 taattttctt ttaaatgata gatatagagt accacatcgc ggctttctat gcgttgttca
    44041 ataaattcga acataggctt cgatttattg cgctatctct gtggcaggat tgtgaataaa
    44101 gtgtcaacaa gcaacggggc atcgcccatt caaacatcta taaggaaaca ccatgagcaa
    44161 ggtattagtt cttaaatcca gcatcctggc agggtactct cagtctaatc agttgtccga
    44221 ttattttgta gaacaatggc gcgaaaagca ctccgttgat gaaatcaccg ttcgcgacct
    44281 ggctgcaaat ccgattccgg tactggatgg cgaactggtt ggcgctctgc gtccgagcga
    44341 tgcgccgctg actccgcgtc agcaggaagc tctggcgctt tccgatgagc tgattgccga
    44401 gctgaaagcc cacgacgtta tcgttattgc ggcaccgatg tataacttca acatctcaac
    44461 tcagttgaaa aactattttg acctggttgc acgcgcaggc gttactttcc gctataccga
    44521 gaacggtccg gaaggtctgg taacgggtaa aaaagccatc gttattacca gccgcggtgg
    44581 gatccacaaa gatggaccaa cggacctggt gacgccgtat ctgtccacgt tcctcggctt
    44641 tatcggcatt accgatgtga aatttgtctt cgccgaaggg atcgcatacg gtccggaaat
    44701 ggcagcgaaa gcgcagtctg acgcgaaagc ggccatcgac agcattgttg ctgcataacc
    44761 ggacacgatc ttgatgtgga ctgtagttca gtccacatct caatccactt acctgttttc
    44821 ccataatttc agcatcgctt tgtgatcttc cttaagcaca atatgcgaac gtctggctcg
    44881 aatataacta atcgcttcat caacggtttt acagtgtccg taacataaca accatgccgc
    44941 cactaccagc gcactgcgcg ataatcccaa cgcgcaatgg accagaacgc tgccttgctc
    45001 ttcgcgtaat gtttccagca tcgccacggc ctgtcggagc tccccctctt ccggaaccac
    45061 cagatccaac atcggtacac agaaatagag tcgatctttt gtcgctcgtc cccgagggaa
    45121 ttcaaaggtg acgtccagaa ccgcattctg tgccggaata tgtcgtggaa acgcccctaa
    45181 ataaacacca gcagtaattt tgctcaccgg ctccaggcga cgacaaaacc aacgcataga
    45241 cagccacatc ccgatgcgcc agggcaatgt cagccagtaa acggcgggcg ggagtttccc
    45301 ctgactatct ttccctgttg ttatcgcgcc aagcccaccg tacccacggc caataatgag
    45361 tagcgataat actggccaac ataaccagac tgaccaccat aactgaatca tcaccattag
    45421 ctccatcaac acaatgcacg agcccgcgcc tacgacatat ggcagtgcta ttttgattcg
    45481 acgttgatca ggtttctgat aattccaacg acgatcgacg ggcaccatcc agtcaatcaa
    45541 catacctacc gccagccctg tgatgacatc aataaaatga tgctgccagg tcgtcagagt
    45601 agaaatagcg atgagtaaaa accatccgcc acagacttta cgccacctct cagccagatg
    45661 ctgacgaaag tgacgccaaa gtagccagca gagaataata tgcagcgaag gagactggtt
    45721 ataaggcaga tcaaacagtt caagttgcga aaatagccat cccgtcaccc cactcacttc
    45781 aggacggata aaactaaact tcagcggata gagcaaaaaa ccgcagcagg ccattaccgt
    45841 tgccagaata agccggtgga caagtcggcg ctgttcgaat gtcgtgctac aaacgaacag
    45901 cgaaaatcca tataaaagat ccagactcca gtaaggaaca atagtccagg gaagaaaagg
    45961 gatcgccgtt tcccaaccga atacctgact ggggatatca tggctgttaa ggtcctgaac
    46021 cgcggtgaac tgattaagag atccataggt gaagaagaaa aacggggcca gcaacaataa
    46081 ccagccagcg ccttgtagca atacgttccg acgttcagtt atcacgacgc accgccatcg
    46141 aaaccgtaaa aataccccac tcatcaatcc gttgtgtgca tttatcaaat ccggcatcac
    46201 gcacgagtga atccatctcc ccttgcgaac gcacgcgcat cacccacggt ttaccatctt
    46261 tatgactggt taacaccccg gcaatcagct ccagttgtgg gtgccacggc tgcccggtgt
    46321 agatgagaat gccacccggt tcgatggcat tggcaagacc tgcgagggag ttttttacct
    46381 gctcattttc gggaaaaagc tcatacaggc cagagacaat cgccagcgta ggccgcggag
    46441 ttaacgcgct gagttcctcc gggttaaacg catcgccctg ttcaaaacgc acctgcccag
    46501 acattccccg ttgggcaatc atctcttgcc cctgtgcaac atttaactca ctgtaatcac
    46561 gtaacaaaat atcgcatacg gcaggttcat tctccagcgc atccagtaca tagcgcccat
    46621 gccctgcggc aatgtcaacc acgcggatgg ctaaaccttt ggcgtggaga tcggcaacgg
    46681 cttgtttaat cagcatttgt aaatgggttt tgcgctggcg aataccgcgc cagccaacac
    46741 tgttcaggta gattttgtcg actaaacgcc cgaatgcgtt actaccttgt ggttgatttt
    46801 gatagacata atccagcgaa ctgcctgaat caaagccggt gctcattccg agatgcaggc
    46861 ccgcagagtg cgccccgaac aatttcatcg ccatacgcat aaagcgatac gccaaatcaa
    46921 ccggcgataa tggcacgggt ccaccagaaa gtagccgcca tcgatccgct gatggtcctg
    46981 tgcagtcttc atgctgataa tcaaattttt gcgatttgtt agcatataaa tgactaataa
    47041 agctttgcat tttttcaaat gccagcgccc tgttctcttc acccaacgtg tcgtgataaa
    47101 agcctggcag cagatgcagc tctttgagag ggctacgtaa acgctgataa aaatcgatct
    47161 gcggttggcg atgcaccaca tagtcatcgc ctgatatcag aagttgcgtg gggagcgtaa
    47221 tcgccgccgc atcgctaaca atacgttcag acgttttata gagatcgagc aagatattga
    47281 cggcaatcgc ccgtgtgatc agcggatcat tattgaaact cgccacccgt tgccgatcgt
    47341 gggtcaaata gcgtcctttc acataggaat taataaaaaa cagaccacgc agacgatgcc
    47401 ataacgccag cgcaggacgt gccagcggca catacaattt aaccttaaag gccggagaag
    47461 ccagcaccag cccgcgaatt gcaggcgcat aatcatgaac ccatgtggca accagcactg
    47521 cgccgacgct ttgcgcgatc acaaccacct cttccagtcc aacctggctg tcgctggcag
    47581 caaaacggac aaattcatcg acatcctgca ctgaacgcgc aagagatggg ctataaccac
    47641 gcggccccga agtttttcca tgcccccggg catcccatgc ataaaaagca gtatctggca
    47701 tcgccagttc atcaacgaga tgttgtagac gaccagaatg ttcatgaccc cgatgaaata
    47761 agacgatgac ctttttcgcc ccgggctgta aagcgggcca gtgccgataa aacaacgctg
    47821 tattatcact ggtggtaaaa aaatgttccc cagggatgcg tgaattttcc attttctcta
    47881 ttttccttca gtaacagctg tagcgaataa aataaaaaaa taccggagcg gtgaaaatta
    47941 atgagtcaat cctgtcaagg agtccgccat gtcctggtaa tagttttcca ctatctttaa
    48001 caccaatatc tcgtttgatg gctgacatca cgacatcgcc gcaaaaacca ctaataccaa
    48061 ttaacaaacc cgctaataat gcctgtaatg tatttagcgg tgtcagcaac ggtccaataa
    48121 tcagtgacgc aatcatggtg gtgatgacgc cccccaccag accttccagc gtttttcccg
    48181 ggctgacttt ggggaccact tttcttctgc cgcaggattt tccccataaa tactgtgcaa
    48241 tatcgtttga ctcggttaat gccaaaagaa ataacaccag taacgccccg ccctggatat
    48301 ttgtggttgg caacattaat aaccaggcgg catgactcaa ggcgaaaacg gtcattatcc
    48361 agccccaaaa aatggcgctc acggtatgca gaaaaccgga ggggtccccc ataaaaactt
    48421 gcccggtggc taatattaga aacccagcca aaggtataaa taatagatac agctcaaagc
    48481 aattaaaacc gataaacaaa tagttaagag gaataaccca atataaccac cgcggaaaat
    48541 gtacagatat aagcgtacaa tattctttca atgccagaaa acttatcagc gcaaaaaacg
    48601 tcaacgtcat ccagcgtgga cctgaaatca ccagtgaaaa acaaatgacg atgccccacc
    48661 aggtacgaat acgtaatgta acttcatcct cttttctctc cggtaaccgc catagcagaa
    48721 aatggtttat cagcgttgct aaaattaaca acgcgaaaag tgttgccaga gatttttcca
    48781 gcattatctt tcagcgctca taagaagcac actccgacaa cgattaatcg cagtccagag
    48841 aagcagaatt gaagcaatgc tccataaaag attattccac tgcatccact gtggataaat
    48901 ggcaacggcc agaccccaca gaccaaatat taacgcgcga tcgcttttgc caaatggacc
    48961 ggcataactg cgaacaccgt taatcgtctg cgcgagtaaa ccgcaaaact cggtcaatat
    49021 ggtgcaaaat aacatgagta tcacgagtga tgcgttactt tccggtaaaa ataaaaatgg
    49081 taaatagagc gcaatatcgg aaataacatc accagtctca ttcaaaattg ccccaagccg
    49141 tgtttgctga ttgcactcac gcgccaacat gccatccagc gcattgagcg ccatacggat
    49201 aaaaagcacg atgggcaata gcataaagag gatgggttgt gccaccaaca tcaacagcaa
    49261 tccggtaaga agagaaagcg ccagtgcagc aagagtgatg tgattcgctg taacgtggtg
    49321 cttgtaaagc caaaacatcg tcggccttaa cagcgactga aagagcggtt ttatctggta
    49381 gagcgtcatt atcaatcctt gataattcgg tttgcgctat ggtgcatcaa ttgtgatggc
    49441 ggagatatca caacgcggag catcaacgta aattcggaat atatcaagat gtggtggatg
    49501 gttttcctac acatctgttt gattaccgac tggatgtcag tgagatacca tcgatcactg
    49561 tacgaatcaa tgaatccatt gccttttccc tgattatcca gccgcaaatc ctggccttaa
    49621 acatatgcac tccatgccat taaaaaagaa acaaaaggga aatgcatctt tcccttttgt
    49681 atattcgtta ttctcgcgaa ataccatcca acgtagacaa cacttcctca gaaagatgca
    49741 atttttcagc agccatattc tcccgtaaat gcgcaacaga agacgtccct gggatcagca
    49801 aaatattcgg tgaacgttgt aacagccacg ccagcgccac ctgcatgggt gttgcaccca
    49861 ggctcgcagc aacatccgac agcgtagagg attgcagcgg tgtaaagccc ccgagcggga
    49921 agaacggcac gtaggcaatg ccgtcacgag ccaaagcatc aatcattgcg tcatcagcac
    49981 gatgagcaat gttgtattcg ttctgcacac agacgatttc ggcaatcttg cgcgcctctg
    50041 caacctgcgt cggtgtgacg ttgcttaggc caatatgttt taccaggcct tgttgttgca
    50101 tctctgccag cacggtcagg ctggcttcaa tcgatccttc cgctgggcca tgaccatccc
    50161 ccatcataat gcgcaagtta accacatcca gtacgtccag cccgagatta cgtagattat
    50221 cgtgcaccgc tttttgcagc tctgccggag aaaatgcggg caaccaggat gcgtcctctc
    50281 cacgccgcgc accaatttta gtgacaattg tcaggtcgtc agagtaagga taaagcgctt
    50341 cgcggataat ctgattggtg acgtgcggac cataaaagtc gctggtatca atgtgattga
    50401 cgcccaatgc cagcgcctcg cgcagcacgg ttatagcgac gtggcgatct cttgggggac
    50461 caaaaacccc aggacctgcc agttgcatcg cgccataacc aagacggtta acggattttg
    50521 taccgagagt aaatgtattg ctgctcatcg caccctgccc tgtaataaaa cggattaaga
    50581 aaaatcagta tagaaacgaa aagagataaa taatgcgagg gcgtaaacaa gattactgga
    50641 tgagttgctc tattgtcctg aaaaacacaa aacaaaacgc gtaaagcgcc gcatccggca
    50701 ataaaaaaac cgcaacctgt atcagcgttg cggtttaggt atcacatgct aaaaattaga
    50761 atgttcagta tctggacgtt actgtttcgg tcgcagattc tcatcaaata ccagacgttt
    50821 cctgttcggt tcaacttccc gcaatggctc aacttgatgc aacgtctgct tacaacgagt
    50881 caccagcacc tgatactcat gcgtaccctg ctttttccat gccagctcct gctcactgag
    50941 ttcacgaatc gctttcgccg ggctgccgac aatcaggtaa ttggcaggca tttctgcttt
    51001 ggctttcaca aatgcggatg cgccaacaat gctgttctcg ccaatcaccg cgccgtccat
    51061 cactaccgcg ttcattccca ctaatgcatt gcggcggata atgcaaccat gaaggatagc
    51121 gctatgacca atatgtccat cttcttccac gacagtatcc tgctcgggaa aaccgtgcat
    51181 aacgcaatta tcctgaatgt tcgcgccatc tttcaccacg atacgaccaa aatcgccacg
    51241 caggctggca tttggcccaa cgtaaacgcc cttgcctaga ataacatcgc cgatcaatac
    51301 cgctgtcgga tggacaaaac tctcttctgg cacaaccgga gtcagaccat ctatctggta
    51361 aattggcata tcgcctcctg ttcaatattc aaaccgccaa aacgttgaaa atacaggctt
    51421 cccggcgcag gcagttcacc gaccgaggtt tcaccttttt cactaacgaa cgctaaagca
    51481 gcaggcgcta cgcgctgata aatgttgata cacagctgac gcgccgtatg ccctgcccag
    51541 tgcgccggaa gcaactcctc tggcaacaat gggtctttaa ggacgacacg gcgataaaaa
    51601 tggatcagta aaagctgaat atgaaatgca cgctccgggg ttaactcgtc tgctgcctct
    51661 tttaacagcg gcaccagcgg gcggaatgac tgaataaagg tttcgtacat ggcattttgt
    51721 tcagttaaat gccagcactc ttccactctg gcacgcagtg ctgcacgaga aagcgccagt
    51781 ggtatttgcg cttcaaaaca aatcacgtta tccaccaccc ccgcttcatg caaaagtgtc
    51841 tgtacatccg ccagtttttg cgacggcgat gccatcaggc tgggtgccag tgcgccaaaa
    51901 ccttgccaga tcaactgctt tttgacatca gccagcgttg atttatctaa accttccgag
    51961 agcaacagga gccatttacc atcccatgca ggttgctctg cgcgataaat tttactttct
    52021 gcccgtcgcg tcaggcgcaa gcctttatca ctgaggctat agaaactgcg tcgcccgatg
    52081 cgggaaacat ccagccagcc ttctttatta agacgaaaca aagcggtgcg cacgaaacgc
    52141 tcaccaaatc ccagcccttc cagcaaagca gccagactac ccagccagat ttcaccacca
    52201 cgatgggaaa gcgaatcacc atacagagag gagatcaaag atgtgccact gaccggaaca
    52261 gcgtttacag catgttggat aaaagtaaca agtttactca ttcgattcag tctgttgtta
    52321 ttgttgtgat ttatgaatca tagcacaggt cgtatcgact tctggccctg gcgctttacc
    52381 tcaccagggc cagacgacac gtcaggcacc aacaatattg cgcagatcaa acacccgaca
    52441 cgccttgcct tctgaacgcg ggatactgcc acagttaacg atcatcacat cggtagagat
    52501 ccccaccatc gacttaatcc gatggcgcag ctggtggcac acctggcaac gctgctcatg
    52561 cgtcaatgtc aggctgcttt ctttcaactc cactttcaca gaaagtgaat caagatgccc
    52621 ccggcggttc acctccagtt ggtaatgggg cgataaatgt tcgaatttga caatctcttc
    52681 ttccagttgt gacgggaaga catttacacc gcgaatgatc aacatgtcat cgctgcgtcc
    52741 gctgatgcga tccatgcggc gcatagtccg cgccgttcct ggtaacagtc gggttaaatc
    52801 acgcgtgcgg taacgaatga ccggcaatgc ttctttggtc agcgtggtga ataacagttc
    52861 gccatgttca ccatcggcaa gcggtgtgcc gtcatgagga ttaacaattt caggatagaa
    52921 atgatcttcc caaatggttg ggccgtcggt agtttccaga cactccatcg ccacccccgg
    52981 ccccatcact tctgacaggc cataaatatc cagtgcggtg atccccagac gccgttcaat
    53041 ctctttacgc atggcctgtg tccacggctc agcaccaaac actccaaccc gcagcgagca
    53101 accgctggca tcaccgccca actgccgctc cagctcttca atcaggttaa ggcaataaga
    53161 tggcgtaacc atgatcatat ctggttgaaa atcgcggatc agttgcgctt gtttttccgt
    53221 ctgcccacca gacatcggga tcaccgtagc gcccagacgt tcggcaccat agtgcgcgcc
    53281 cagcccacca gtaaacaggc cgtaaccata ggcaacatga attttgtctt tgggcgtgcc
    53341 acctgccgca cgcaaggaac gcgctacaat attggcccag ttatcaatat cgttttgcgt
    53401 atagccgaca actgtcggtt tacctgtggt tcccgaagaa gcatgaatgc gcaccacttg
    53461 ttccatcggc acggcaaagg tgtcgaaggg atagttgtcg cgcagatcct gtttggtggt
    53521 acagggaaat ttacgcagat ctgaaagttc cctgaaatca tcaggatgta cgcctgctgc
    53581 gtcaaatttg cgccgataca tcgggacatt ttcatacgcg tgtttgagcg tccatttcag
    53641 acgttgtgtt tgcaacgcct gtaactcatc aacagacgcg gtttcaatcg ggtcaagctt
    53701 tgtattggtt atcattgtag ggtactcgca ggttgatatg ctcaaacacg ctccagaatc
    53761 atggcgatgc cctgaccgac accgatgcac atggtgcaca atgcgtaacg accgttacgc
    53821 cgatgcagct catggctggc agccagtgcc aggcgggcac cactcattcc caacggatgg
    53881 cctaaggcga tagcgcctcc gttggggtta acatgtgggg catcatcagg cagccccaat
    53941 tcgcgtagta cacccaacgc ctgggccgcg aacgcttcgt tcagttcaat cacgtccata
    54001 tcgtgaatac tcagccccgc gcgttccagc acccggcgag ttgcaggcac cggaccaagc
    54061 cccatcaggc gcggttccac cccggcggtt gccatggcta cgattcgcgc ccgcggtgtc
    54121 agtccttgcg ctgctgccat ctgttcactg gcaataatta acgcagcggc tccgtcattc
    54181 accccggagg cattgcctgc ggtaatcacc ccattggcac gaaatggtgc ttttaaccca
    54241 cgtaactgtt ccagcgtcgt ttccgggcgc agatgctcat catgttgtat ttctgttaca
    54301 acgcctttct tgtttttcaa cacaaccgga acaatctcct cagccagaat gcctgaggat
    54361 tgcgcttttg ccgtacgttg ctgactgcgt agcgcaaaac tatcttgatc ttctcgcgat
    54421 atttttaaca gttcagctac attctctgcc gtttccggca tgctgtcagt tccaaattgt
    54481 tgagccatga gcgggttcac aaatcgccag ccaatagtgg tatcaaacat ctcagcctga
    54541 cgagaaaatg cactggttgc cttgcccata acaaacggtg cccgagacat tgactccacg
    54601 ccaccagcga tcagcaaatc gccatcgccc gctttaatcg cccgtgcggc gaaccccagt
    54661 gcgtccagcc cggaaccaca caagcggtta atggttgtgc cggaaacact ctgcggcagc
    54721 cccgccagta acgtcgccat ccgggctacg ttacggttgt cttctcccgc ctgattagca
    54781 cagccgagga tcacatcatc gatacactcc gcatcgagac gcgggtttcg caccagcagc
    54841 tcccgcaaag ggatagcagc cagatcgtca gcccgaacac ctgataatgc cccaccatag
    54901 cggccaattg gcgtacgaat tccgtcacag ataaaggctt cacgcatcag gcttctcctg
    54961 taatggtgcc gccgatgcgg tgagatttac cgcgaaacag cgcaaccgtt ttttgttgtt
    55021 ggttaacaat ttcgatgtcg taaacaccgg tttgcttgcc ctgatgacgt acctgcgcag
    55081 tagcagttaa ggtgtctccg gcaaagcctg gacgtaaaaa atcaatcgtg caggcagaag
    55141 cgacggctgc cagcccctgg ctattgcagg cgtaggcaaa ggcagtatca gccagtgaaa
    55201 atagctgccc gccgtggcaa ctttgatgac cgttaagcat ttgtgcagtg acggtcatgg
    55261 tcactacagc aaagccttca tccattgaga taatgtcgat gccaagcgct ttggcgcagg
    55321 catcgttctc atacattgca tgggcatttt gccaggcctt atgactcata accgctctcc
    55381 agaagcgccc gttggcgcag caatgaacat gggcgatagc gttcttcgcc atagtgatgc
    55441 tgtagatttt caaggagcct taatattcgc tgccagccaa gttgcgctcc ccaggcaagt
    55501 gggccatatg gataattccc cccaagacgc atggcggtat cgatatcctg ttcagaggcc
    55561 acgccttttt gaagcgcatc aagggcttca ttgatgatca ttgctaccgt tcgccaaatc
    55621 agcattcctg ggtaatctgc aatttgcagc actgttttgc cctgctgttg caggtaataa
    55681 atggccttgc gggtcgctga gtccgggttc accgctgccg cagcaatggt caccaccttg
    55741 cccgccattt tgtcgaccac taccaccggg cgtgccagtc gtattgccag cgcctgtgcc
    55801 gtctcgcctt gtgtctcaat caataaaaca tcgtcaattt ccgtgacacc gtcacttttc
    55861 ttttctactt tcattgggct aaaactgtcg cttaccgctt ccaggccaac aactgcctca
    55921 cgttccgcgc gccagtcgta cacgcccagc ccacttttct tgcccaaccg tccaccaatc
    55981 accagttcct gttgcaccag cgaaggtaaa aaacgacgct cctgccagaa agcgttaaac
    56041 accgaacagg tgacagcaaa attgacgtcc tgaccaatca gatcggttaa ttccagcggc
    56101 cccatcggga aaccagcgcc atcgcgaagt gcagcgtcaa tcacttctgg tgcagcaacc
    56161 tgctcttcca gtgcccgcca ggcctcggaa taataaggac gcgcaacacg gttaacgata
    56221 aatccggggg tcgaatgaca gcgcactggc tgcttacccc aactcaatgt cagttcacac
    56281 aactgctcga caacttccgc tgccgttgcc agcccactga ccacctccac caacttcatc
    56341 accggtgccg ggttaaaaaa atgcagcccc gcaacacgtt caggattttt tacctccgca
    56401 gcaatcgcgg ttatagagat tgacgaagtg ttagtggtca atagcgtttg tggcgggcaa
    56461 acttccgcca gttgtgcaaa gagcgctttt ttaacttcca gacgttcaga cgccgcttca
    56521 ataaccagat tcgctgctgc cagcgcgtga atatcggtca ccgggatcag gcgtttcaat
    56581 gtgcgttcac aggtttcagc ggtcagtttt ccccgcgtca cgcgtgaatt tagccgcgcg
    56641 tgtatcccgt cgattgcgcg ggtcagcgct tcagcagaaa tgtcatacag taaaacctga
    56701 tgtccatgac tggcggcaac ttcagcaatg cctgccccca tggtgccgct cccaatcact
    56761 gccacagttt gcacatttat catcatgcta tttccccgtg aactgcggtg agcgtttagc
    56821 caggaacgca ctgacacctt cacgataatc tgcgctccgt ccggcaagtc gctgatagtc
    56881 acgttccaga tccagttgcg tatcgagcgt attggtttca gcgctattta tcgcttgctt
    56941 gataagtcca agaccaaatg tcggttgtgt cgccagatgc cgtgccagct gttgcgcggt
    57001 atctgccagc gtttcatcat caacaacctg ccagatcatc ccccattcgt gcgcctgttc
    57061 agcactcagt tgattcccca gcagtgccag ccccatggcg cgcgctcgtc cggcaacgcg
    57121 tggcagtaac caggttccac cgcaatcggg tattaagcct aacttactaa acgccatgac
    57181 gaattttgct gaacgggcag caataacgat gtcgcacccc agtgccagtg ttgcgcctgc
    57241 ccctgccgcc acgccattga ctgcacagat caccggtttt ggcagttttg ccaggcgacg
    57301 taccagtggg ttatagaaac gttcaactga catccctaaa tccggtgcgg ggcctgtggg
    57361 atcaacgtta cgatcgttaa gatcctgacc agcacaaaat ccgcgcccgg cacctgtaag
    57421 taacaggcaa cggatagtgt cgtcgcgctc gacctgtttc aggcactctg ccagttgtgc
    57481 gtgcatctca tcattaaaac tgttcaggcg ttccgggcgg ttgagcgtta gtgtcatcac
    57541 gcccttttct acatgactga ggatgaattc catcattagc gtcctttaaa gtcgggcgtg
    57601 cgtttttgta agaaagcgga gatgccttca tgacgatctt ctgttgccgc cagcaaggtg
    57661 aataactgtc gctcctgggc aagtccggct tgcaaagcca cttcctgcga ctggcgcagc
    57721 gcttgctttg ccgcttgtaa ggccagcggc gagtgacgtg ccattttcga tgccagctgt
    57781 aaggcgtatt cgagggttaa atcgctgggg aagacgtcgc taaccagccc ggcctgctgt
    57841 gcttgctggg cggtgatact ttctccgctc agcaccattt tgctggctaa cgatttaccg
    57901 acactacgga ttaaacgttg cgttccgcct gcgccaggca tgatgccgag agtgatttcc
    57961 ggcaacccaa aacgcgcgtt ctctccggca accaccacat cgcacaacaa tgccagttcg
    58021 caacccgccc caagcgcgta accattgacg gctgcgataa gtggtttatt aaaggcctgc
    58081 aatcgcgccc atagctgcgg acgtgtatcg tttaaggtgg ccgcgagatc tttttctgcc
    58141 atttcgttga gatcggcccc agcggcaaaa aagcgtgcat taccggtaat cacacagacc
    58201 gaaatgctgg tatcggtagc cgcagcttcc agttcattta ccagttgcat cagcagggca
    58261 ttatttagcg cattacgtgc ggcgggacgg ttaagggtca gcaacaatac tcgttgctga
    58321 cggctgacga tcagttcgct catgccatcc ccttcgcgtc aaagtcaacc accacatcac
    58381 tggtcagcgg cagtgcctgg caactcaaca catagcctgc agccagttca tccggttcca
    58441 gactgtaatt ggtttccatc gccactttgc cacgcagcac tttgcatttg caggtcgcac
    58501 agacgccgcc tttgcaggca tagggcagat cagccccctg gcgcaatgcc gcatcgagaa
    58561 tgctttcatc gccggcatta agcacgattt cccgatcccg cccatcctga cgtacagtca
    58621 ctttttgtcc gtcactttgc acgttaacgc tacgtttgac gcgcgtgcca ggcgtattaa
    58681 accgctccag atgaatggtt ttatctggca ttcccagtgc ttttaaggcg gcttccgtct
    58741 catccatcat cgccgccgga ccacaaataa atgcctcatc ataaagacga aaattaatga
    58801 gcgaggcccc aagtgactgt aatttttcac cgtcaatacg cccgtgaagc agatcgctgt
    58861 cgagggtttc ctgactgaaa atgcacaaca actgtaaacg ctgaggatat ttgtctttca
    58921 ggtctgccag tgcctggcga aacatcatgc tctggctggt acggttaccg tagatcaggg
    58981 tgaactgact ttcaggctcg gtttgtaaag tggcggcgat aatcgccagc attggcgtaa
    59041 tacctgatcc tgctgcaata gccagatagc gcccctggcg ttcggcctgc ggctgatagc
    59101 cgaaatggcc ctgcggcacc atgacttcca gcgtcatccc ctgacggatg tgatcgcggg
    59161 catagcggga gaaacgtccg ccttcaatgg cttttaccgc cacactaatt tcgccaggca
    59221 ggtagctgcg gcaaatggag taacaacggc gtaattcttc accatcaagg ctggctttta
    59281 aggtcaagtg ttgaccgggg cgaaagcgat acgcctcctg caaaggctgg ggcaccgcaa
    59341 aggtaatggt caccgcatca cgggtttccg gctccacttt tgccaccgtt aaggaatgaa
    59401 acgttgtcat ggcatcctca aatacatttg aaataatcga aaggttcgcg gcaactatcg
    59461 cagcgataca atgctttgca ggccgtggaa ccaaattcac tgataagtgt ggtatggacg
    59521 ctggcacagc gcgggcaaca tacttctggc ggcaaatggg catggcaact gtgtccggcg
    59581 ggcgggctaa tgccatactg tcgcagacgc tcacgggcat cgggggtcat ccagtcggtg
    59641 gtccatgccg ggtcaagttg cagcacaacc tgaacggggg taaacccgtg ggttgacatt
    59701 gcctcacgta tcgcgccaat caaatgttcc gttgccggac aaccggaata tgtcggcgta
    59761 aagccgatca cccatccttc tcccatctgt gtcacattac gcaccatgcc taaatcagta
    59821 atggtcagca ccgggatctc cgggtccggg atctggctga gtagcgccca tatctcatga
    59881 acctgtggcg gtgcaatggt ggccagacgt tgcataccca tctcctctgt taccattgct
    59941 gaccgggcaa gacacgctgg agatactgca tttctgccag catgggtcca aggtgttcgg
    60001 tatgcagtcc ttttttaccg ccagtgcgat acgcctgctc ttgcggtacg ttcaatgtgg
    60061 cttcgttgat cccagcaaaa acttcggctt cccacgctgc gcgtaaagtg cgtggatcaa
    60121 ccgcaatacc ctcttcactc agtgcaatat caatctcgtc ggcatcgaac agttcggcgg
    60181 taaaacgcca caacttgttg atggcctgct gcatcttttg ccctgatacg tcagtaccat
    60241 tgcccagtcg ctccagccag ccacgactaa aacgcaggtg ataacgcgct tctttaattg
    60301 ctttggcaga aatcgccgcc agttgcggat cacggctctc catcagacgg gtaaagagcg
    60361 ccacatgcca tgcatcgatg aaatactggc gtgcaatggt gtcggcaaaa ttgccgtttg
    60421 gctgttcaac caacaataag ttgctgaact ggcgctcgtc tcgggtgaag gccagggtat
    60481 cttcatcgcc ttctcccgct aattcagcgg catacgataa gaagttgcgt gcctggccta
    60541 ataaatcgag tccaatattt gccagtgcga gatcgatttc cagttccggt gcgtgaccgc
    60601 accattcccc caaccgctgg gagagcacca gacagttatc gcccaggcgc aaggtgtaag
    60661 ccgttaactg attcatttcc gacctcacat gtgctcaatg ccatcaggga tggtgtaaaa
    60721 cgttggatgg cgatagacct tgctttcagc cggatcaaaa aattcaccgc gttcttccgg
    60781 ttgcgaggca acaatttcac tcgccttcac cacccaaatt gaacatcctt cgctacgacg
    60841 ggtgtaagca tcacgggcat tttccagtgc catccgctca tcggcagcat gtaaactgcc
    60901 aacatggcgg tgtgataagc cctgtttgcc acgcacgaaa acttcgtata acggccagta
    60961 aacattactc attttgaatc tccttatgcg accttacggg caagttgttt ttgtgcatgg
    61021 gccagcgctg cttcccgtac ccaggtacct tcttcccagg ctttacgttt ggcgtcgagc
    61081 ctctcctgat tacaaattcc gcgaccgtta attacttcgt taaactcctg ccagtcgatc
    61141 tcaccaaagc ggtagtgacc gctttcagtg tcaaaatgca gatccgggtc aggaacggtc
    61201 ataccgagca tttcaacctg tggaacggtg ttgtccacga aacgctggcg gagttcgtcg
    61261 ttggtgaaac gtttgatttt ccaggcgaga cttctggcgc tgtttggcga gttatcatcg
    61321 tttggcccga acatcattaa ggctggccac cagaaacggt taatggcgtc ttgcaacatc
    61381 tgcttttgag cttcgctgcc ctgcgccagt gccatgcagg cttcaaaccc ctgacgctgg
    61441 tgaaaactct cttctttgca gattttcacc attgctctgg catacgggcc ataagaagtg
    61501 cggcataacg ccacctgatt gacgatcgct gcgccatcca ccagccagcc gataacaccg
    61561 atatcagccc aactcaatgt cggataatta aagatggagg aatatttcat ccgtccgtcg
    61621 agcatttttt ggtagatgtc ttctctggcg cagcccagtg tttcggcggc gctatagaga
    61681 tacagaccat gaccggcttc atcctgcact ttggccaaca gaatggcttt acgccgcaag
    61741 gttggtgcgc gagtgatcca gttaccttca ggcaacatac caacaatctc ggagtgcgca
    61801 tgctgcccaa tctggcggat cagtgtcttt cggtaagcat cgggcatcca gtcctgcggc
    61861 tcgatagccg tttcctgggc tatccgttgc tcaaagcgtt cttcttgggt cacgtcatca
    61921 cctttacgat tccagttttc gtgaattaat taaaaacacg aatcacgttg ttttatgaaa
    61981 gttacacaat ggttaactaa aatcacaaat tttgtatgtt tgaactgtga cggatttcgc
    62041 accctatttt tataactcat tatatatcag tgcattaatg tttttagtgg ttgatataca
    62101 gatcgcatag ttaacatttc gttaaaatgc gccttgcttt ttatgattcg tgatttaact
    62161 attagcaaca gaaatgtgaa acatctggag agtagcgatg cagcagttag ccagtttctt
    62221 atccggtacc tggcagtctg gccggggccg tagccgtttg attcatcacg ctattagcgg
    62281 cgaggcgtta tgggaagtga ccagtgaagg tcttgatatg gcggctgccc gcctgtttgc
    62341 cattgaaaaa ggtgccccag cccttcgcgc tatgaccttt atcgaacgtg cggcgatgct
    62401 taaagcggtc gctaaacatc tgctgagtga aaaagagcgc ttctatgctc tttctgcgca
    62461 aacaggcgca acgcgggcag acagttgggt tgatattgaa ggtggcattg ggacgttatt
    62521 tacttacgcc agcctcggta gccgggagct gcctgacgat acgctgtggc cggaagatga
    62581 attgatcccc ttatcgaaag aaggtggatt tgccgcgcgc catgtactga cctcaaagtc
    62641 aggcgtggca gtgcatatta acgcctttaa cttcccctgc tggggaatgc tggaaaagct
    62701 ggcaccaacg tggctgggcg gaatgccagc catcatcaaa ccagctaccg cgacggccca
    62761 actgactcag gcgatggtga aatcaattgt cgatagtggt cttgttcccg aaggcgcaat
    62821 tagtctgatc tgcggtagtg ctggcgactt gttggatcat ctggacagcc aggatgtggt
    62881 gactttcacg gggtcagcgg cgaccggaca gatgctgcga gttcagccaa atatcgtcgc
    62941 caaatctatc cccttcacta tggaagctga ttccctgaac tgctgcgtac tgggcgaaga
    63001 tgtcaccccg gatcaaccgg agtttgcgct gtttattcgt gaagttgtgc gtgagatgac
    63061 cacaaaagcc gggcaaaaat gtacggcaat ccggcggatt attgtgccgc aggcattggt
    63121 taatgctgtc agtgatgctc tggttgcgcg attacagaaa gtcgtggtcg gtgatcctgc
    63181 tcaggaaggc gtgaaaatgg gcgcactggt aaatgctgag cagcgtgccg atgtgcagga
    63241 aaaagtgaac atattgctgg ctgcaggatg cgagattcgc ctcggtggtc aggcggattt
    63301 atctgctgcg ggtgccttct tcccgccaac cttattgtac tgtccgcagc cggatgaagc
    63361 accggcggta catgcaacag aagcctttgg ccctgtcgca acgctgatgc cagcacaaaa
    63421 ccagcgacat gctctgcaac tggcttgtgc aggcggcggt agccttgcgg gaacgctggt
    63481 gacggctgat ccgcaaattg cgcgtcagtt tattgcagac gcggcacgta cgcatgggcg
    63541 aattcagatc ctcaatgaag agtcggcaaa agaatccacc gggcatggct ccccactgcc
    63601 acaactggta catggtgggc ctggtcgcgc aggaggcggt gaagaattag gtggtttacg
    63661 agcggtgaaa cattacatgc agcgaaccgc tgttcagggt agtccgacga tgcttgccgc
    63721 tatcagtaaa cagtgggtgc gcggtgcgaa agtcgaagaa gatcgtattc atccgttccg
    63781 caaatatttt gaggagctgc aaccaggcga cagcctgttg actccccgcc gcacaatgac
    63841 agaggccgat attgttaact ttgcttgcct cagcggcgat catttctatg cacatatgga
    63901 taaaattgct gctgccgaat ctatttttgg tgagcgtgtg gtacatggat attttgtgct
    63961 ttctgcggct gcgggtctgt ttgtcgatgc cggtgtcggt ccggtcatag ctaactacgg
    64021 gctggaaaac ttgcgtttta tcgaacccgt aaaaccaggc gataccatcc aggtgcgtct
    64081 cacctgtaag cgcaagacgc tgaaaaaaca gcgtagcgca gaagaaaaac caacaggtgt
    64141 ggtggaatgg gcggtagagg tattcaatca gcatcaaacc ccggtggcgc tgtattcaat
    64201 tctgacgctg gtggccaggc agcacggtga ttttgtcgat taatcggtga atgaagggca
    64261 acggcgaata gttgcccttt tatttcacta aattttgtga cgttgtcaca ttatgcatga
    64321 tgtgtacatc tattttcagg gcatccactg tatgaaaagc tggcacacct gccaaacaac
    64381 ctggcaggtg caggcaatcc cctttgcatc agtactgata atgtgaacct gactaaaccg
    64441 cccacagagc gcggttgcta acaagaacac aacatctgac gaggttaata atgggaagcc
    64501 cctctctgta ttctgcccgt aaaacaaccc tggcgttggc agtcgcctta agtttcgcct
    64561 ggcaagcgcc ggtatttgcc cacggtggtg aagcgcatat ggtgccaatg gataaaacgc
    64621 ttaaagaatt tggtgccgat gtgcagtggg acgactacgc ccagctcttt accctgatta
    64681 aagatggcgc gtacgtgaaa gtgaagcctg gtgcgcaaac agcaattgtt aatggtcagc
    64741 ctctggcact gcaagtaccg gtagtgatga aagacaataa agcctgggtt tctgacacct
    64801 ttattaacga tgttttccag tccgggctgg atcaaacctt tcaggtagaa aagcgccctc
    64861 acccacttaa tgcgctaact gcggacgaaa ttaaacaggc cgttgaaatt gttaaagctt
    64921 ccgcggactt caaacccaat acccgtttta ctgagatctc cctgctaccg ccagataaag
    64981 aagctgtctg ggcgtttgcg ctggaaaaca aaccggttga ccagccgcgc aaagccgacg
    65041 tcattatgct cgacggcaaa catatcatcg aagcggtggt ggatctgcaa aacaacaaac
    65101 tgctctcctg gcaacccatt aaagacgccc acggtatggt gttgctggat gatttcgcca
    65161 gtgtgcagaa cattattaac aacagtgaag aatttgccgc tgccgtgaag aaacgcggta
    65221 ttactgatgc gaaaaaagtg attaccacgc cgctgaccgt aggttatttc gatggtaaag
    65281 atggcctgaa acaagatgcc cggttgctca aagtcatcag ctatcttgat gtcggtgatg
    65341 gcaactactg ggcacatccc atcgaaaacc tggtggcggt cgttgattta gaacagaaaa
    65401 aaatcgttaa gattgaagaa ggtccggtag ttccggtgcc aatgaccgca cgcccatttg
    65461 atagccgtga ccgcgttgct ccggcagtta agcctatgca aatcattgag gctgaaggta
    65521 aaaattacac cattactggc gatatgattc actggcggaa ctgggatttt cacctcagca
    65581 tgaactctcg cgtcgggccg atgatctcca ccgtgactta taacgacaat ggcaccaaac
    65641 gcaaagtcat gtacgaaggt tctctcggcg gcatgattgt gccttacggt gatcctgata
    65701 ttggctggta ctttaaagcg tatctggact ctggtgacta cggtatgggc acgctaacct
    65761 caccaattgc tcgtggtaaa gatgccccgt ctaacgcagt gctccttaat gaaaccatcg
    65821 ccgactacac tggcgtgccg atggagatcc ctcgcgctat cgcggtattt gaacgttatg
    65881 ccgggccgga gtataagcat caggaaatgg gccagcccaa cgtcagtacc gaacgccggg
    65941 agttagtggt gcgctggatc agtacagtgg gtaactatga ctacattttt gactggatct
    66001 tccatgaaaa cggcactatt ggcattgatg ccggtgctac gggcatcgaa gcggtgaaag
    66061 gtgttaaagc gaaaaccatg cacgatgaga cggcgaaaga tgacacgcgc tacggcacgc
    66121 ttatcgatca caatatcgtg ggtactacac accaacatat ttataatttc cgcctcgatc
    66181 tggatgtaga tggcgagaat aacagcctgg tggcgatgga cccagtggta aaaccgaata
    66241 ctgccggtgg cccacgcacc agtaccatgc aagttaatca gtacaacatc ggcaatgaac
    66301 aggatgccgc gcagaagttt gatccgggca cgattcgtct gttgagtaac ccgaacaaag
    66361 agaaccgcat gggcaatcca gtttcctatc aaattattcc ttatgcaggt ggtactcacc
    66421 cggtagcaaa aggtgcccag ttcgcgccgg acgagtggat ctatcatcgt ttaagcttta
    66481 tggacaagca gctttgggta acgcgttatc atcctggcga gcgtttcccg gaaggcaaat
    66541 atccgaaccg ttctactcat gacaccggtc ttggacaata cagtaaagat aacgagtcgc
    66601 tggacaacac cgacgccgtt gtctggatga ccaccggcac cacacatgtg gcccgcgccg
    66661 aagagtggcc gattatgccg accgaatggg tacatactct gcttaaacca tggaacttct
    66721 ttgacgaaac gccaacgcta ggggcgctga agaaagataa gtgattgttt cagacaaaaa
    66781 aacggcacca ggtgccgttt tttacttatg agcgaaccag attaataccg tacacacacc
    66841 gacttagttt cacaccaacc gtccagccag tcggggccaa aatcacggcc cgttcctgac
    66901 tgcttcatcc caccaaacgg taagttagcg tcaattaagg tatggctgtt tacccacacc
    66961 gtccctgcct gtaagcgatc gctatattcc agagcctggg agagattttg cgtccagaca
    67021 ctggcagtta agccatattc cgtgtcgttt gccagttgta acgcctcttc tccatccgct
    67081 actcgcacca ggtttaccac cggaccaaac acctcttcac gagttaagcg caatttagca
    67141 tcgggattta ccaccagcgt tggcgcaaca taatccccct ctccggctgg tccattcgac
    67201 ccgcgaatca gctctgcttg ctgtgcctgc gcatcgtcga ggaatgaaca cactttgtcg
    67261 cagtgcgcac gagaaaccaa agggttaatc tgtgcaacag gtgacatccc cggtcccact
    67321 tgcaacgatt ttaccgcctg ctcaaatcca ctaaccagcg tgtcaaacaa cggcgcttca
    67381 atataaattc gcgaactggc ggcgcatact tgcccttgat tcaggaagct tccggtcatc
    67441 aagccttcaa taacccattg cggatcagca tcttttaata caattgccgg gtttttaccg
    67501 cccagttcca gcgttacacg cgttaagtga tcagcagcag ttctggcaat accttttccc
    67561 gtcgcggttg aaccggtaaa actgattttc gcaacatgag gatgtgacgt cagggccgcg
    67621 ccgcatacag cacctgaccc ggtgacgaca ttaaaaacgc catcagggat accagcctcg
    67681 ctagccagtt ccgccacgcg caacatcgtc agtggcgtgg tttccgaagg cttaatcacg
    67741 attgaacagc ctgctgccag tgctggcatc accttccaca taccaatcat caacggaaag
    67801 ttccatggca caattcccgc cactacgcca accggctctt tacgcgtcca ggcctgataa
    67861 cgcgccccct ggggtaaggg aatcgacaag tccagcgttt tacccgcgat tttggtcgtt
    67921 aacccggcgg tataacgcat ccagttcagc gtacagccca cttcaaaagc acgggaaatg
    67981 gcaattgact tgccttgctc cagggtttcc agttgcgcca gctcctcact gtgctgttcc
    68041 accagatcgg caaaacgtag cagaatacgt tcacgctctg cgggtaatcg cccggcccag
    68101 cgacgcgaga caaaggcccg ccaggcagac atgactgcgt tatctacatc cgcttcgttg
    68161 gcatcagcag tagacgcaat ttcttgcccg gtggccggat caaagatcgc caaccgtttt
    68221 tcactttgtg cggggccagg acgaccatca atataaagac cgtgttgacg atcgagaaac
    68281 tgttggacct ggcttaatac tgctacatgc ggctctgtca tcacttttcc ttattattta
    68341 cccagtgtga tttcagtgta cgcagcgaca agaaaagtca cttttttcgc tgtgacattc
    68401 gctttgtcag ctgtgacaag ctccgcaaat cgtgacaata accgcttatg tctctatgcc
    68461 tgacaagact tatttcctat agtcaggctc attagcttcg tttattgcaa cacaaatgca
    68521 acaataaaaa tacatttcac agagcgaaaa cgtgccatga accccgcaat ggataatgag
    68581 tttcagcaat ggctttccca aatcaatcag gtatgcggaa attttaccgg acgcctgctg
    68641 actgagcgtt acacgggtgt actggacacg cattttgcca aaggactaaa gctgagtacc
    68701 gtgacaacca gcggggtgaa tttatcccgc acctggcagg aggtaaaagg cagcgacgat
    68761 gcctggtttt acaccgtttt tcagcttagt ggtcaggcaa taatggagca ggatgagcgt
    68821 caggtgcaga ttggcgctgg cgatattacg ttactcgatg cctcacgccc ctgttcgctt
    68881 tactggcagg agtcttctaa acagatttca ttacttttgc cacgcactct gctggaacaa
    68941 tattttcccc atcaaaaacc tgtctgcgca gaaagactgg acgctgactt acccatggtg
    69001 caactcagtc atcgcctgtt acaggagagc atgaataatc cggcactttc tgaaacagaa
    69061 agtgaagctg cgctacaggc gatggtgtgt ctgctgcgcc cggtacttca tcagcgggaa
    69121 tctgttcaac ctcgtcgtga acgtcagttt caaaaagtgg ttacgttgat agacgataat
    69181 attcgcgaag agatattacg cccggagtgg atagccggag agacaggtat gtcagtacgt
    69241 agtttgtacc gaatgtttgc cgataaaggt ttggtggtcg cgcaatatat tcgtaaccgt
    69301 cgtctcgatt tttgtgcaga tgcgattcgc catgccgcag atgatgaaaa actggcaggc
    69361 atcggctttc attggggatt ttctgaccag agtcattttt caacggtatt taagcaacgc
    69421 tttgggatga cgccaggcga gtatcgacgt aaattccgct aactaattgg cgttgtgtgt
    69481 actgcaacgc caattagtta aaacagatat ttgactatgc atttttcagg gcgaaaggca
    69541 atttgacagg agtttttgaa tattcacctt gtttcaggaa aatagatcaa tcgctttctc
    69601 ctgtagcgtt atcccgatat tgcggttttg ctattaaaat tttcgcaccc atttaccgtt
    69661 tatcccttgt acatattccc ccggtttggc ccgagctacg agcttttgcc cggcgagttt
    69721 cgcgatatca tctacagaca cattattttg ctttgccagt tgttggtaac tcgctttacg
    69781 ttcggcatta atgtcggtta ctaatttctc tgtttcagca tccgttttca gcgcaaccag
    69841 ataaccataa aatgtttcac ctacccgccc ctgagttctg gcttcatcca gagtcaat
//
LOCUS       DAJOWB010000012        66468 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJOWB010000012 DAJOWB010000000
VERSION     DAJOWB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.012"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(1..585)
                     /locus_tag="OHO85_000880"
     CDS             complement(1..585)
                     /locus_tag="OHO85_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2670301.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            complement(955..1434)
                     /gene="folK"
                     /locus_tag="OHO85_000881"
     CDS             complement(955..1434)
                     /gene="folK"
                     /locus_tag="OHO85_000881"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCQ2670302.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(1431..2849)
                     /gene="pcnB"
                     /locus_tag="OHO85_000882"
     CDS             complement(1431..2849)
                     /gene="pcnB"
                     /locus_tag="OHO85_000882"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCQ2670303.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(2888..3814)
                     /gene="gluQRS"
                     /locus_tag="OHO85_000883"
     CDS             complement(2888..3814)
                     /gene="gluQRS"
                     /locus_tag="OHO85_000883"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCQ2670304.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            complement(3851..4306)
                     /gene="dksA"
                     /locus_tag="OHO85_000884"
     CDS             complement(3851..4306)
                     /gene="dksA"
                     /locus_tag="OHO85_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCQ2670305.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(4484..5188)
                     /gene="sfsA"
                     /locus_tag="OHO85_000885"
     CDS             complement(4484..5188)
                     /gene="sfsA"
                     /locus_tag="OHO85_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCQ2670306.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(5203..5733)
                     /gene="thpR"
                     /locus_tag="OHO85_000886"
     CDS             complement(5203..5733)
                     /gene="thpR"
                     /locus_tag="OHO85_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCQ2670307.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            5807..8236
                     /gene="hrpB"
                     /locus_tag="OHO85_000887"
     CDS             5807..8236
                     /gene="hrpB"
                     /locus_tag="OHO85_000887"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCQ2670308.1"
                     /translation="MSSLPVAAVLSELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKDLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            8432..10966
                     /gene="mrcB"
                     /locus_tag="OHO85_000888"
     CDS             8432..10966
                     /gene="mrcB"
                     /locus_tag="OHO85_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCQ2670309.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            11186..13375
                     /gene="fhuA"
                     /locus_tag="OHO85_000889"
     CDS             11186..13375
                     /gene="fhuA"
                     /locus_tag="OHO85_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCQ2670310.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIANDGHTLNRGTVVDNERLQNFSVDTQLESKFATGEVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGNAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRVSYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            13426..14223
                     /gene="fhuC"
                     /locus_tag="OHO85_000890"
     CDS             13426..14223
                     /gene="fhuC"
                     /locus_tag="OHO85_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCQ2670311.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPTEGEILLDTQPLESWSSKAFARKVAYLPQQLPPAEGLTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            14223..15113
                     /gene="fhuD"
                     /locus_tag="OHO85_000891"
     CDS             14223..15113
                     /gene="fhuD"
                     /locus_tag="OHO85_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCQ2670312.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            15110..17092
                     /gene="fhuB"
                     /locus_tag="OHO85_000892"
     CDS             15110..17092
                     /gene="fhuB"
                     /locus_tag="OHO85_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCQ2670313.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLIIAVVVALSFGRDAHGWTWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(17127..18407)
                     /gene="hemL"
                     /locus_tag="OHO85_000893"
     CDS             complement(17127..18407)
                     /gene="hemL"
                     /locus_tag="OHO85_000893"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCQ2670314.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            18632..20053
                     /gene="clcA"
                     /locus_tag="OHO85_000894"
     CDS             18632..20053
                     /gene="clcA"
                     /locus_tag="OHO85_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCQ2670315.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            20077..20142
                     /locus_tag="OHO85_000895"
     CDS             20077..20142
                     /locus_tag="OHO85_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670316.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            20135..20479
                     /gene="erpA"
                     /locus_tag="OHO85_000896"
     CDS             20135..20479
                     /gene="erpA"
                     /locus_tag="OHO85_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCQ2670317.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(20526..21149)
                     /locus_tag="OHO85_000897"
     CDS             complement(20526..21149)
                     /locus_tag="OHO85_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCQ2670318.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(21187..21987)
                     /gene="btuF"
                     /locus_tag="OHO85_000898"
     CDS             complement(21187..21987)
                     /gene="btuF"
                     /locus_tag="OHO85_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCQ2670319.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21980..22678)
                     /gene="mtnN"
                     /locus_tag="OHO85_000899"
     CDS             complement(21980..22678)
                     /gene="mtnN"
                     /locus_tag="OHO85_000899"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCQ2670320.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIEKRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            22762..24279
                     /gene="dgt"
                     /locus_tag="OHO85_000900"
     CDS             22762..24279
                     /gene="dgt"
                     /locus_tag="OHO85_000900"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCQ2670321.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCYFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            24409..25833
                     /gene="degP"
                     /locus_tag="OHO85_000901"
     CDS             24409..25833
                     /gene="degP"
                     /locus_tag="OHO85_000901"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCQ2670322.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            25988..27145
                     /gene="cdaR"
                     /locus_tag="OHO85_000902"
     CDS             25988..27145
                     /gene="cdaR"
                     /locus_tag="OHO85_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCQ2670323.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(27234..27620)
                     /locus_tag="OHO85_000903"
     CDS             complement(27234..27620)
                     /locus_tag="OHO85_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCQ2670324.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27935..28759)
                     /gene="dapD"
                     /locus_tag="OHO85_000904"
     CDS             complement(27935..28759)
                     /gene="dapD"
                     /locus_tag="OHO85_000904"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCQ2670325.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28790..31462)
                     /gene="glnD"
                     /locus_tag="OHO85_000905"
     CDS             complement(28790..31462)
                     /gene="glnD"
                     /locus_tag="OHO85_000905"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCQ2670326.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31524..32318)
                     /gene="map"
                     /locus_tag="OHO85_000906"
     CDS             complement(31524..32318)
                     /gene="map"
                     /locus_tag="OHO85_000906"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCQ2670327.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32686..33411
                     /gene="rpsB"
                     /locus_tag="OHO85_000907"
     CDS             32686..33411
                     /gene="rpsB"
                     /locus_tag="OHO85_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCQ2670328.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33669..34520
                     /gene="tsf"
                     /locus_tag="OHO85_000908"
     CDS             33669..34520
                     /gene="tsf"
                     /locus_tag="OHO85_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCQ2670329.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34667..35392
                     /gene="pyrH"
                     /locus_tag="OHO85_000909"
     CDS             34667..35392
                     /gene="pyrH"
                     /locus_tag="OHO85_000909"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCQ2670330.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35542..36099
                     /gene="frr"
                     /locus_tag="OHO85_000910"
     CDS             35542..36099
                     /gene="frr"
                     /locus_tag="OHO85_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCQ2670331.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36191..37387
                     /gene="ispC"
                     /locus_tag="OHO85_000911"
     CDS             36191..37387
                     /gene="ispC"
                     /locus_tag="OHO85_000911"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCQ2670332.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSCTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37576..38334
                     /gene="ispU"
                     /locus_tag="OHO85_000912"
                     /gene_synonym="uppS"
     CDS             37576..38334
                     /gene="ispU"
                     /locus_tag="OHO85_000912"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCQ2670333.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38347..39204
                     /gene="cdsA"
                     /locus_tag="OHO85_000913"
     CDS             38347..39204
                     /gene="cdsA"
                     /locus_tag="OHO85_000913"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCQ2670334.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39216..40568
                     /gene="rseP"
                     /locus_tag="OHO85_000914"
     CDS             39216..40568
                     /gene="rseP"
                     /locus_tag="OHO85_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCQ2670335.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40598..43030
                     /gene="bamA"
                     /locus_tag="OHO85_000915"
     CDS             40598..43030
                     /gene="bamA"
                     /locus_tag="OHO85_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCQ2670336.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43152..43637
                     /gene="skp"
                     /locus_tag="OHO85_000916"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43152..43637
                     /gene="skp"
                     /locus_tag="OHO85_000916"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCQ2670337.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43641..44666
                     /gene="lpxD"
                     /locus_tag="OHO85_000917"
     CDS             43641..44666
                     /gene="lpxD"
                     /locus_tag="OHO85_000917"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCQ2670338.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44771..45226
                     /gene="fabZ"
                     /locus_tag="OHO85_000918"
     CDS             44771..45226
                     /gene="fabZ"
                     /locus_tag="OHO85_000918"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCQ2670339.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45230..46018
                     /gene="lpxA"
                     /locus_tag="OHO85_000919"
     CDS             45230..46018
                     /gene="lpxA"
                     /locus_tag="OHO85_000919"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCQ2670340.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            46018..47166
                     /gene="lpxB"
                     /locus_tag="OHO85_000920"
     CDS             46018..47166
                     /gene="lpxB"
                     /locus_tag="OHO85_000920"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCQ2670341.1"
                     /translation="MTKQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47163..47759
                     /gene="rnhB"
                     /locus_tag="OHO85_000921"
     CDS             47163..47759
                     /gene="rnhB"
                     /locus_tag="OHO85_000921"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCQ2670342.1"
                     /translation="MIEFVYPHTHLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47794..51276
                     /gene="dnaE"
                     /locus_tag="OHO85_000922"
     CDS             47794..51276
                     /gene="dnaE"
                     /locus_tag="OHO85_000922"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCQ2670343.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51289..52248
                     /gene="accA"
                     /locus_tag="OHO85_000923"
     CDS             51289..52248
                     /gene="accA"
                     /locus_tag="OHO85_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCQ2670344.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52347..54488
                     /gene="ldcC"
                     /locus_tag="OHO85_000924"
     CDS             52347..54488
                     /gene="ldcC"
                     /locus_tag="OHO85_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCQ2670345.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            54545..54934
                     /locus_tag="OHO85_000925"
     CDS             54545..54934
                     /locus_tag="OHO85_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCQ2670346.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54999..56297
                     /gene="tilS"
                     /locus_tag="OHO85_000926"
     CDS             54999..56297
                     /gene="tilS"
                     /locus_tag="OHO85_000926"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCQ2670347.1"
                     /translation="MTLTLNRQLLTLRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDIAHCQKPQGTLQIAPMLAMSDARRAAIIRRWLAGKNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGEKGVSFVWQKTLS"
     gene            complement(56346..56606)
                     /gene="rof"
                     /locus_tag="OHO85_000927"
     CDS             complement(56346..56606)
                     /gene="rof"
                     /locus_tag="OHO85_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCQ2670348.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56593..56793)
                     /locus_tag="OHO85_000928"
     CDS             complement(56593..56793)
                     /locus_tag="OHO85_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCQ2670349.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56959..57504
                     /locus_tag="OHO85_000929"
     CDS             56959..57504
                     /locus_tag="OHO85_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCQ2670350.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKFVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57501..57923
                     /gene="arfB"
                     /locus_tag="OHO85_000930"
     CDS             57501..57923
                     /gene="arfB"
                     /locus_tag="OHO85_000930"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCQ2670351.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57937..58647
                     /gene="nlpE"
                     /locus_tag="OHO85_000931"
                     /gene_synonym="cutF"
     CDS             57937..58647
                     /gene="nlpE"
                     /locus_tag="OHO85_000931"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCQ2670352.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58802..59626)
                     /locus_tag="OHO85_000932"
     CDS             complement(58802..59626)
                     /locus_tag="OHO85_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCQ2670353.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGENN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSADFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59679..61397)
                     /gene="proS"
                     /locus_tag="OHO85_000933"
     CDS             complement(59679..61397)
                     /gene="proS"
                     /locus_tag="OHO85_000933"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCQ2670354.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(61508..62215)
                     /gene="tsaA"
                     /locus_tag="OHO85_000934"
     CDS             complement(61508..62215)
                     /gene="tsaA"
                     /locus_tag="OHO85_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCQ2670355.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLMAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPP"
     gene            complement(62212..62616)
                     /gene="rcsF"
                     /locus_tag="OHO85_000935"
     CDS             complement(62212..62616)
                     /gene="rcsF"
                     /locus_tag="OHO85_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCQ2670356.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62734..63549)
                     /gene="metQ"
                     /locus_tag="OHO85_000936"
     CDS             complement(62734..63549)
                     /gene="metQ"
                     /locus_tag="OHO85_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCQ2670357.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63589..64242)
                     /gene="metI"
                     /locus_tag="OHO85_000937"
     CDS             complement(63589..64242)
                     /gene="metI"
                     /locus_tag="OHO85_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCQ2670358.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(64235..65266)
                     /gene="metN"
                     /locus_tag="OHO85_000938"
     CDS             complement(64235..65266)
                     /gene="metN"
                     /locus_tag="OHO85_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCQ2670359.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            65454..66026
                     /gene="gmhB"
                     /locus_tag="OHO85_000939"
     CDS             65454..66026
                     /gene="gmhB"
                     /locus_tag="OHO85_000939"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCQ2670360.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
     gene            66390..>66468
                     /locus_tag="OHO85_000940"
     rRNA            66390..>66468
                     /locus_tag="OHO85_000940"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15854 a        16415 c        18171 g        16028 t
ORIGIN      
        1 ttactgataa gtaatggtgt atgcagtgtt agtttttaca taaccagcag ttgctgtttg
       61 gtctgcgacg gcacgaacgt aagacgcttt aaaatcataa acagcagatt ttgtcgttgc
      121 atccagggct ttagtataca catcgccagg gttgttaatt tgaacctgtt tgatagtgga
      181 accatcaata ttgtgcagag cgatattcac gccgtcagac gggttgttaa tagacatgtc
      241 gttgttcagc gttcctttgc tattaccaaa gaaaacagaa ccgaaggtca ttttagctgt
      301 actacctgga ttcggatttg ctttgctgca gtcaacggtg atgctgaaag gtttggcttt
      361 agcgccaacg gtgtcattca gcacgccagc atcgatttca ccaacagttg cagtttgcag
      421 caggatcaga ccatctttat taccgccgtc aacgcgggtt tcacaggtgt tatcaacaac
      481 cagaccactg atatttaact gaccaccatc catatcagca gaagcagtac ctgcaaccat
      541 tgccagcata agaccagaca gggcaaaacc taatttttta gacataaaaa tccttgaata
      601 aaattccatt agtcagacta ctggtttgaa gaatgactat tcatgacaca aatgttataa
      661 gcagatgcta gatattaatg agcaatgata tttgttacca aaatttacaa ccattgttca
      721 ttaggacgcc tattgtgcac tttaagggct tttgaacaaa ttaaattttc ttaattcaaa
      781 attaagcaaa aataaattca caagtgcaat tggttagggt atggcgatgg gattgatatt
      841 tatctgcggg aatttatatt atgtttctgc aaaaatgttt aaactaaaat ttttattaag
      901 gaaagagagg atgtatgatt ttcagaaaat atttatattc gtaatataaa taaattacca
      961 tttgcttaat ttgtcaaatg ctcttgtatg taagacttca cgcaacgtct ccccatcagg
     1021 aaacgccagc tccggtgcaa tctcaaacag cggccacagc ataaatccac gattcttcat
     1081 atcgtagtgc ggaacggtca ggcgttcggt atttatcact tcattaccaa acagcatgat
     1141 atcgagatcc agcgtgcgtg gtccccagcg ttcagcttta cgaacacgac cctgttgcag
     1201 ttctatgcgc tgtgtatggt tgagtaactc ttccggggcg agcgaagttt caagcgccac
     1261 ggcggcgttg aggtaatcgg gttgatcttg cggccccagc ggtggggtgc ggtaaaacga
     1321 agaaacggca agaatgtggc tttcagggat atcgcctaat gctttcaggg cagcattgac
     1381 ctgctccagc ggagaggcca gattgctgcc tatggcaata tacgccactg tcatgcggta
     1441 ccctcacgac gcggtgcgcg tttgcgtgga cgacgagtac ggcgacgcgg tgacggctct
     1501 tcatccaact cgttaagcat ccctttttga tctggtggcg cggaaacctg gaactcaccc
     1561 caccatttca ccagacgctg cagttcagcg ttacgttcaa cttcagctcg caaggccaac
     1621 aggtcataag ccgcacggaa cttaggatgc tccagcagtt tccatgcgcg tttaccctga
     1681 cgacgggaca tacgcaactg caactgccag atatcgcggg ttaacgtcgt cagacgtttc
     1741 gggattgcca gtgaacggca ggcttcgtcc agcacgtcgt tcatcgccag cgcgaaagcg
     1801 tcgtgatagg tcaggccgct ttcctgggca atcttctgtg ccgtctccag cagtgggtac
     1861 cagaacatgg cggcaaacag gaacgccggg ttcacgcgca tatcgttatg gatacgcgta
     1921 tcggtattct tcagcacctg ttcaatgatc cgctccatcg ggctgtcgcc attttccgtg
     1981 aagtagcggg taatggtcgg gaacagcggc tggaacagat gatattcaca caacagctta
     2041 taggtatcgt aaccgtagcc cgcttgtagc agtttaagcg attcttcaaa caggcgtgcc
     2101 ggtgggatat cgttcaacag ggtagcgagg cgagggatcg gttctgcggt ttccgggctg
     2161 atgcgcatac ccaatttggc ggcaaaacgt accgcgcgca gcatacgtac cggatcttca
     2221 cggtagcgcg tttccgggtt accaatcaga cggataacgc cgtccttcag atccttcata
     2281 ccgccaacgt aatcacggac ggtaaaatcc gctacgctgt aatagaggct gttgatagtg
     2341 aaatcgcggc gctgggcgtc ttcttcgatg gagccgaaaa tgttgtcgcg cagcaacatg
     2401 ccgttttgcc cgcgttggga ggtcgtgcgg tcgctgacgt taccttcgtg gtgtccacgg
     2461 aaggtcgcaa cttcgataat ctccgggcca aacattacat gagccagacg gaaacggcga
     2521 cccaccaggc ggcagttacg gaacagtttg cgcacctgct caggcgtggc gttagtggtt
     2581 acgtcaaaat ctttcggctt tttgccaagt aacaggtcgc gcacgccgcc gccaaccagc
     2641 caggcttcgt atcccgcttt gttgagcctg tacattacct tcagggcatt ttcactgata
     2701 tctttgcggg aaatagcatg ctgctcacgc gggatcaccg tcacctgtgg acgggcgact
     2761 gcctgttcag cctcgctttc ctcgcggctt agcaccttgc ggcaaaaatt agcgactcgg
     2821 gtaaaaatag tacacctcgg tagtgtcaaa catcattcag gacaaaaaaa tagcggctaa
     2881 tcatagctca gcatgacgca tttgagaatg ttgaatttac aattgccgac tcgggcacgg
     2941 cggtaagccg ccagtttttg acggctgact gaaggatttg ctcgacgctg aaatcctgcc
     3001 agtgtgcttc tgcctgctgc cccagaaatt gaagtgccgc gattagtacc gggcgtggat
     3061 cgcctttcgg caacgcaggc gcatgattct gcttggaaag tttagcgcct tgtggattaa
     3121 gcgccagcgg cagatgaatg taatctggca ctttccagcc aaaaagctgg tacagcgaga
     3181 tttgccttac tgttggttca atcagatcag ccccacgcac tatttctgta acgccctgga
     3241 aatgatcatc aaccacaaca gccaggttgt aggcgaacaa cccatcacgg cgatgaatga
     3301 taaaatcttc ccgtgccagt ttttcgtcgg cgtgaataat gccgcgcagc tggtcagtaa
     3361 attgcgtgac cggatgctgc tggcggatac gcactgcggc gttgtctggt ccatgatgca
     3421 acacccggca atgaccgtcg taaataccgc caatgctttg aatacgcgca cgcgtacagg
     3481 tgcagtaata acttagtcct tgttcatgta accaggcgag tgcttcacga taggcgtcgt
     3541 gacgttgcga ttgccagaga acatcgccgt cccagtgcag accgtaatgt tccagctggc
     3601 gcaggatagt ttctgcggca ccgggaactt cacgaggcgg gtcgatatct tctatgcgta
     3661 ccagccagcg accttgccgg gcgcgagcct gcaaatagct gccgagcgcg gcgatcagag
     3721 agccaaaatg aagctcgccg gaaggagagg gggcgaagcg gccaatatac tgtgtgtctg
     3781 tcatctcttt gaacaaaaaa taaggcggga gcatttcccg cctgtggtaa acgtgatgga
     3841 acggctgtaa ttagccagcc atctgttttt cgcgaatttc agccagcgtt ttgcagtcga
     3901 tgcacagatc ggctgtcggg cgcgcttcca gacggcgaat accaatttca acaccgcagg
     3961 attcgcagta gccgaaatct tcgtcttcca cttttttcag cgtcttctcg atctttttga
     4021 tcagcttacg ctcgcgatcg cggttacgca gttcgaggct gaactcttct tcctgggctg
     4081 cacggtctac cgggtccggg aagttggctg cttcatcctg catatgtgta acggtgcgat
     4141 cgacttcatc cctgagttga ttacgccatg cttccagaat acgacggaag tgcgccagct
     4201 gggcttcatt catatactct tcgcccggct tctcctgata tggttccacc ccagcgatgg
     4261 cgagaatact cagggacgat gttttacggt tttgcccttc ttgcatgttg cttctcctta
     4321 acacgcacta tcgatcccca tgttcggggg aaaaatgagg ccgctataaa tagcagatgc
     4381 ttttccggat agcaattatc taaacgtaac acttgacaac tgtgtgagga aaagcgtatt
     4441 tgcgcacgcg accagaatgt aaattaacca gttacttact ttactacaat gtaaccggca
     4501 gtgatttttt aagagccatg ccttcagcag aaatttccgc tttgtaagcc agaatttcta
     4561 cccccctctg ttgagcttct gacaatagtt gcgcgtattt ctcatcgatg tggcgcgcgg
     4621 gtgaaaaccg tgtaatggct gaatgcagca cggcgaaaaa gataaccgca cgctggcctt
     4681 cagccgctac gctcatcaac tcccgaaggt gtttctgacc tcgttcagtg accgcatcgg
     4741 gaaaatatcc ctgttcgttc tccgctaacg taaccgattt cacttcaata tagcagtctg
     4801 gacgcgaatc cgcctgcaac ataaagtcaa tacggctgcg ttctgcgccg tattttactt
     4861 cgcttttcag cgagctatag cctgacagtt ctgaaattga ttcattaagg atagcctctt
     4921 tcgtcaacct gttagcccaa agcgtgttga cgcaaataaa tgcgccgctc tggctttgag
     4981 ttaattccca ggtgtgtggg tatttccgtt tggtgttgtc tgaagtcgaa taccagacgg
     5041 tatcgccagg cgttgcacaa ccggtcattg cacccgtatt cgggcagtgt agcgttaatt
     5101 cgcgaccatc gggtgtgatc acatcggcta aaaaacgttt gtaacgctga attagcgtcg
     5161 cgcgctgtag agggggagag aattccattg cgacaatcct tgttattgcg ttagcgccca
     5221 gcgttttagc ggcgtgtagc gtgtgcgtcc acgggcaaac gaggaggcgt aaagggtgaa
     5281 ctccgtcacc gcataagacc agttaaaacc tggcggcggg attgtcaccg cctcgctggc
     5341 gtcgcgcaat aaggtaatat gtggatgaaa cggacgattg ctttgaaaac aaccgctgcg
     5401 ggcagcctgt gaacggagca tattcgccag ctggattaag ccgcgtggag gctgacgcat
     5461 ccctaaccac accacacgcg aacgcagcca ttgtccggcg tcatcaagcg tgagtgtgaa
     5521 accaggttga cgaatccgtc cggctaaaag agaaagcgcc ttctctttct cggcgctcac
     5581 ttcgcctaaa aatgccagag tcagatgcaa attatcggcg gcgaccggac gtcccgcctc
     5641 aggtgggaag tgtgtggcgc gccaatggat aatctgttcg cggatttctg caggtaagtc
     5701 gatagcaaag aacagacgtt gcggttcaga catgtgaggc actcggttat gaattaccgc
     5761 gatgctacaa tgtggcgcga agaatgttaa ccctctggag cgttttgtgt cgtcgttgcc
     5821 cgttgctgcc gtcttatctg aattacttac cgctctcgat tgtgcgccgc aggtattatt
     5881 aagtgcgccg accggggccg ggaaatcaac ctggctgccg ctgcaactgc tggcgcatcc
     5941 cggcattaac gggaaaatta tcctgctgga gccgcgtcgt ctggcggcgc gtaacgtcgc
     6001 gcaacggctg gcggagctgc ttaacgaaaa gccaggcgat accgttggct accggatgcg
     6061 tgcgcaaaac tgcgtcgggc cgaatacccg cctggaagtg gttaccgaag gcgtgctgac
     6121 gcgcatgatc cagcgtgacc cggaactgag cggtgttgga ctggtgatcc ttgatgaatt
     6181 tcatgagcgc agcttgcagg cggatttggc gttggcgctg ttactcgatg tgcaacaagg
     6241 tctgcgtgat gaccttaaac tgctgattat gtcggctacc ctggacaacg accgcttgca
     6301 gcaaatgctg ccagaagcgc ctgtcgtcat ctcagaaggg cgctcgtttc cggttgaacg
     6361 ccgttattta ccgctgcccg cgcatcagcg ttttgacgat gccgttgcgg tagccaccgc
     6421 tgaaatgctg cgtcaggaaa gcggatcatt actgttattt ttacctggcg tcggagaaat
     6481 tcagcgtgtg caggaacaac tggcttcgcg catcggcagt gatgtattgc tctgcccgct
     6541 gtatggcgcg ttgtcgctga acgatcagcg aaaagcgatc ctcccggcac cgcaagggat
     6601 gcgcaaagtg gtgctggcga ccaatattgc tgaaaccagt ttaaccattg aaggtattcg
     6661 tctggtggtg gattgtgccc aggagcgtgt ggcgcgtttt gatccgcgca cggggcttac
     6721 gcgactgatt actcaacgcg ttagccaggc atccatgacg cagcgtgccg ggcgcgccgg
     6781 gcgtctggag ccgggtatct gcctgcattt aatcgccaaa gaacaagcag aacgcgccgc
     6841 ggcgcaaagt gaaccggaga tcttacaaag cgatctttcc ggtttgctga tggaattact
     6901 gcaatgggga tgcagcgatc cggcgcagat gagctggctg gatcaaccgc cagtagtgaa
     6961 tctactggcc gcgaaacgtc tgttacaaat gctgggggca ctggagggtg aacggcttag
     7021 tgcgcaaggg caaaaaatgg cagcgctggg taacgatccg cgtttagcgg caatgctggt
     7081 tagcgcgaag aacgacgacg aagctgctac cgcggcaaaa attgccgcca ttctcgaaga
     7141 gccgccacgg atgggcaata gtgacctggg cgtggcgttt tcgcgcaatc aaccagcctg
     7201 gcagcaacgt agtcagcaac tgttaaaacg cttaaacgta cgtggcggtg aggcagacag
     7261 ttcgcttatc gcgccgctac ttgccggggc gtttgccgat cgcattgctc gtcgccgtgg
     7321 gcaagatgga cgctatcaac tggcaaacgg catgggagcg atgctcgatg ccaacgacgc
     7381 gctaagccgc cacgaatggt tgatcgcacc gttattattg cagggcagcg cctcgccgga
     7441 tgcgcggatt ttactggcgc tgccggtcga tattgatgag ttagtacaac gctgcccgca
     7501 gctggtacag cagtctgaca ctgtggagtg ggatgacgcg caaggtacgc tgaaagcctg
     7561 gcgtcggctg caaatcggtc tgctgacggt gaaagtgcag ccgctggcga aaccctcgga
     7621 agacgagttg catcaggcga tgcttaatgg catccgcgat aaagatttaa gcgtgctcaa
     7681 ctggacggcg gaagcggaac agctacgctt gcgtttgtta tgcgccgcaa agtggttgcc
     7741 ggaatatgac tggccagcgg ttgatgatga aagtttattg gcgacgctgg aaacgtggct
     7801 gctgccacat atgactggcg tacattcact acgcggcctg aaatcactcg acatttatca
     7861 ggcactacgc ggattacttg attggggaat gcagcaacgt ctggatagtg aattgcctgc
     7921 gcattacact gtgccgacgg gaagccggat cgccattcgt tatcatgaag ataacccgcc
     7981 cgcgctggcg gtgagaatgc aagagatgtt tggcgaggcc accaatccga cgatcgccca
     8041 ggggcgcgtg ccgctggtgc tggagttgct ttcacctgcc caaaggccat tacaaatcac
     8101 acgagatttg agcgccttct ggaaaggagc gtaccgtgag gtgcaaaaag agatgaaagg
     8161 gcgttatccc aaacatgtct ggccggacga cccggcaaat actgcaccga cgcgacggac
     8221 gaaaaagtat tcgtaaggtt ggttttctcc ctctccctgt gggagagggc cggggtgagg
     8281 gcatcagcgc gcacgttcac cctcacccta accctctccc tcaagggaga ggggaccgat
     8341 cgagcacaaa ttttgagaga tatcttcttc tgtcttgtaa cagaagaaca gaaaatcggg
     8401 cttttgcgcc tgaatattgc ggagaaaaag catggccggg aatgaccgcg agccaattgg
     8461 acgcaaaggg aaaccgacgc gtccggtcaa acaaaaggta agccgtcgtc gttacgaaga
     8521 tgacgatgat tacgacgatt atgatgacta tgaggatgaa gaaccgatgc cgcgcaaagg
     8581 taagggcaaa ggcaaagggc gtaagcctcg tggcaaacgc ggctggctat ggctgctgtt
     8641 aaaactggct atcgtttttg ccgtgctgat cgccatttac ggcgtttatc tcgatcaaaa
     8701 aattcgtagc cgtattgatg gcaaggtctg gcaactgcct gcggcagttt atggccgaat
     8761 ggtcaacctt gagccagata tgaccatcag caagaacgag atggtgaagc tgctggaggc
     8821 gactcagtat cgtcaggtgt cgaaaatgac ccgtcctggc gaatttaccg tgcaggccaa
     8881 cagcattgag atgatccgcc gtccgtttga tttcccggac agtaaagaag ggcaggtgcg
     8941 tgcgcgtctg acctttgatg gcgatcatct ggcgacgatc gtcaatatgg agaacaaccg
     9001 tcagttcggt ttcttccgtc ttgatccgcg tctgatcacc atgatctcct cgccaaacgg
     9061 tgagcagcgt ttgtttgtgc cgcgcagtgg tttccctgat ttgctggtgg atactttgct
     9121 ggcgacagaa gaccgtcatt tttacgagca tgacggcatc agcctctact ccatcggacg
     9181 tgcggtgctg gcaaacctga ccgccggacg cacggtgcag ggagcgagta cgctgacgca
     9241 acagctggtg aaaaacctgt tcctctccag tgagcgttct tactggcgta aagcgaacga
     9301 agcttacatg gcgctgatca tggacgcgcg ttacagcaaa gaccgtattc ttgagctgta
     9361 tatgaacgag gtgtatctcg gtcagagcgg cgacaacgaa atccgcggct tcccgctggc
     9421 gagcttgtat tactttggtc gcccggtaga agagctaagc ctcgaccagc aggcgctgtt
     9481 agtcggcatg gtgaaaggag cgtccatcta caacccgtgg cgtaacccaa aactggcgct
     9541 ggagcgacgt aatctggtgc tgcgtctgct gcaacagcaa cagattattg atcaagaact
     9601 ctatgacatg ttgagtgcac gtccgctggg tgttcagcca cgcggtgggg tgatctctcc
     9661 acagccagcc tttatgcaac tggtgcgtca ggagctgcag gcgaaattag gcgataaggt
     9721 aaaagatctc tccggcgtga agatcttcac tacctttgac tcggtggccc aggacgcggc
     9781 agaaaaagcc gccgtggaag gcattccggc actgaagaaa cagcgtaagt tgagcgatct
     9841 tgaaactgcg attgtggtcg tcgaccgctt tagtggtgaa gttcgtgcga tggtcggtgg
     9901 ttctgagccg cagtttgcgg gctacaaccg tgcgatgcag gcgcgtcgtt cgattggttc
     9961 ccttgcaaaa ccagcgactt atctgactgc cttaagccag ccgaaaatct atcgtctgaa
    10021 tacgtggatt gcggatgcgc caatcgcgct gcgtcagccg aatggccagg tctggtcacc
    10081 gcagaatgat gaccgtcgtt atagcgaaag cggcagagtg atgctggtgg atgcgttgac
    10141 ccgttcgatg aacgtgccga cggtaaatct ggggatggcg ctggggctgc ctgcggttac
    10201 ggagacctgg atcaaactgg gcgtaccgaa agatcagttg catccggttc cggcgatgct
    10261 gctgggggcg ttgaacttaa cgccaatcga agtggcgcag gcgttccaga ccatcgccag
    10321 cggcggtaac cgtgcaccgc tttctgcact gcgttcggta attgcggaag atggcaaggt
    10381 gctgtatcag agcttcccgc aggcggaacg cgctgttccg gcgcaggcgg cgtatctgac
    10441 actatggacc atgcagcagg tggtacaacg cggtacgggt cgtcagcttg gggcgaaata
    10501 cccgaacctg catctggcag ggaaaacagg gactaccaac aataacgtag atacctggtt
    10561 tgcgggcatt gacggcagca cggtgaccat cacctgggta ggccgtgata acaaccagcc
    10621 gaccaaactg tatggtgcca gcggggcaat gtcgatttat cagcgttatc tggctaacca
    10681 gacgccaacg ccgctgaatc ttgttccgcc agaagatatt gcagatatgg gcgtggacta
    10741 cgacggcaac tttgtttgca gcggtggcat gcgtgtcttg ccggtctgga ccagcgatcc
    10801 gcaatcgctg tgccagcaga gtgagatgca gcagcaaccg tcaggcaatc cgtttgatca
    10861 gtcttctcag ccgcagcaac agccgcaaca gcaacctgct cagcaagagc agaaagacag
    10921 cgacggtgta gccggttgga tcaaggatat gtttggtagt aattaacatc taagcgtgaa
    10981 ataccggatg gcgaattgcc atccggtaaa ataacatccc acctaaagta ttaacccttt
    11041 cttttcatct ggttgtttat taacccttca ggaacgctca gattgcgtac cgcttgcgaa
    11101 cccgccagcg tttcgaatat tatcttatcc ttataataat cattctcgtt tacgttatca
    11161 ttcactttac atcagagata taccaatggc gcgttccaaa actgctcagc caaaacactc
    11221 actgcgtaaa atcgcagttg tagtagccac agcggttagc ggcatgtctg tttatgcaca
    11281 ggcagcggtt gaaccgaaag aagacactat caccgttacc gctgcacctg cgccgcaaga
    11341 aagcgcatgg gggcctgctg caactattgc ggcgaagcac tctgctactg cgactaaaac
    11401 ggatacacca attgaaaaaa cgccgcagtc tatttcggtt gtgacaaatg aagagatgca
    11461 gatgcatcaa tttcagtctg taaaagaagc attaggttat acacctggcg ttacggttag
    11521 cagtcgtggt gcttctaata catatgattt tgttatcatt cgtggtttct catctgttgg
    11581 tctgaatcaa aataattacc ttgatggact aaaacttcag ggcaactttt ataacgatgc
    11641 tgtgattgat ccttacatgc tcgagagggt tgaactgatg cgtgggccga cgtctgttct
    11701 ctatggaaaa agtaatcctg gtgggattat ttctatggtg agtaagcgtc cgaccactga
    11761 acccctgaaa gaaattcagt ttaaaatggg gacggataat ctgtttcaga ctggattcga
    11821 tttcagtgat gcactggatg ataacggtga gttctcctac cgtttgacgg gcctcgctcg
    11881 ttcaacaaac gaacagcaga aaaattctga atcccagcgt tataccattg ctccatcatt
    11941 ctcatggcgt ccagacgaca aaaccaattt tactttcctg tcctatttcc agaatgaacc
    12001 agaaacgggt tattacggtt ggttgccgaa agaggggacc gttgagccat tgcctaatgg
    12061 taaacgtcta ccgactgact tcaatgaagg tgcatcgaat aatacatact cccgtaacca
    12121 gaaaatggtg ggatatagtt ttgaacatgg ttttaatgac accttcaccg tgcgtcagaa
    12181 tctgcgtttc agtgaaatga aaacctcaca gaaaagtgtt tatggcacag ggattgccaa
    12241 cgatggtcat actctaaacc gcgggacagt ggtggataat gagcgtctgc aaaactttag
    12301 cgttgatacc caacttgaaa gtaaatttgc tacaggtgaa gtggagcata ctttgctgac
    12361 aggggtagac ttcatgcgta tgcgcaatga tatcaatgcc agctttggat ctgcaccatc
    12421 aatcgatctt tataacaaat atcatcctga atactttgca tttggtaacg cagagccata
    12481 ccaaatgaat gaaagcaaac aaacaggtat ttatgttcag gatcaggcgg aatggaataa
    12541 atgggtattc actctggggg gacgctacga ttggtctaag caagcgacca ctgttcgtga
    12601 aaactcttat acgccgactg aaggttatat tgagcgtaat gatcatcagt tcacctggcg
    12661 cggtggtgta aattacttat tcgataatgg tatttcacct tactttagct atagccagtc
    12721 ctttgaaccg agtgctttcg atctgtggag caacccgcgc gtttcctata agccatcgaa
    12781 aggtgaacag tatgaagctg gcgtaaaata tgttccgaat gatatgccgg tcgttgttac
    12841 gggcgcagtc tatcaattga cgaaaacaaa taacctgaca gcagacccaa caaacccgtt
    12901 agcgcaagtc ccagcaggtg agattcgcgc tcgtggtgtg gagcttgaag caaaagcagc
    12961 gttaaatgcc aatattaact tgacggcttc ttatacctac acggatgcgg aatacaccaa
    13021 agacaccaat ctcaaaggta aaactccaga acaagtaccg gagcatatgg catctctctg
    13081 gggggattat accttcaatg aagggccgct ttctggttta acattgggaa caggtggtcg
    13141 ttttattggt tccagctatg gtgatccggc aaacactttt aaagtgggta gcgcagctgt
    13201 aatggatgct gttgtaaaat atgatctggc acgctttggt atggcgggat ccagccttgc
    13261 tgtgaacgtc aacaatttgc tcgatcgtga gtatgttgcc agttgcttcc agacttatgg
    13321 ctgcttctgg ggcgcagaac gtcaggtcgt tgcaaccgca accttccgtt tctaatttct
    13381 cttttggggc acggattacc gtgcccattt cacaagttgg ctgttatgca ggaatacacg
    13441 aatcattccg ataccacttt tgcactgcgt aatatctcct ttcgtgtgcc cgggcgcacg
    13501 cttttgcatc cgctgtcgtt aacctttcct gccgggaaag tgaccggtct gatcggtcac
    13561 aacggttctg gtaaatccac tctgctcaaa atgcttggcc gtcatcagcc tccgacggaa
    13621 ggggagattc ttcttgatac ccagccgctg gaaagctgga gcagcaaagc gttcgcccgc
    13681 aaagtggctt atttgccgca gcagcttcct ccggcagaag ggttgaccgt gcgtgaactg
    13741 gtggcgattg gtcgttaccc gtggcatggc gcgctggggc gctttggggc ggcagatcgc
    13801 gaaaaagtcg aggaagctat ctcgctggtt ggcttaaaac cgctggcgca tcggctggtc
    13861 gatagtctct ctggcggcga acgtcaacgg gcgtggatcg ccatgctggt ggcgcaggat
    13921 agccgttgtc tgttgctcga cgaaccgact tcggcgctgg atatcgccca ccaggttgat
    13981 gtgctggcgc tggtgcaccg tttaagtcag gaacgtggcc tgacggtcat tgccgtgctg
    14041 cacgatatca atatggcggc acgctactgt gattatctgg tcgccctgcg cggtggtgaa
    14101 atgattgctc agggaacgcc tgcggaaatt atgcgcggcg aaaccctcga aatgatttat
    14161 ggcatcccga tgggtatttt gccgcatccg gcgggtgctg cacctgtgag ttttgtttat
    14221 tgatgagcgg cttacctctt atttcgcgcc gtcgactgtt aacggcgatg gcgctttctc
    14281 cgttgttatg gcagatgaat accgcccacg cggcggctat tgaccccaat cgtattgtgg
    14341 cgctggagtg gttgccggtg gaattactgc tggcgctcgg catcgtgcct tacggcgtgg
    14401 cggataccat caactatcgc ctgtgggtca gcgaaccacc attgccggac tcagtgattg
    14461 acgtcggttt gcgtacagaa cctaaccttg aactgctgac cgaaatgaaa ccgtcgttta
    14521 tggtctggtc ggcaggatat ggcccttcat cagaaatgct ggcacgtatt gcgccgggtc
    14581 gcgggtttaa cttcagtgac ggcaaacagc cgttggcgat ggcgcgtaaa tcgctgacgg
    14641 aaatggcaga tttacttaac ctgcaaagcg cagcggaaac gcatttagcg caatacgaag
    14701 actttatccg cagcatgaaa ccccgctttg tgaagcgtgg tgcacgcccg ttactgctga
    14761 cgacgcttat cgatccgcgc catatgctgg tcttcggtcc aaacagcttg ttccaggaaa
    14821 ttctcgatga gtacggcatc ccaaatgcct ggcaagggga aaccaacttc tggggcagta
    14881 ccgccgtcag tatcgatcgt ctggcggcgt ataaagacgt tgatgtgctc tgttttgatc
    14941 acgacaacag caaagacatg gatgcgctaa tggcaacccc gctgtggcag gccatgccgt
    15001 ttgtccgtac agggcgcttt cagcgcgtac ccgcagtctg gttttatggt gcaacgcttt
    15061 cggcaatgca ctttgtgcgt gttctggata acgccatcgg aggtaaagcg tgagtaaacg
    15121 aattgcgctt ttcccggcgt tattgctggc gctgttagtg attgtcgcta cggcgctaac
    15181 ctggatgaac ttctcacagg cgctgccgcg cagtcagtgg gcgcaggccg cctggtcgcc
    15241 ggatattgac gtcatcgagc agatgatttt tcactacagc ttgttgccgc gtctggcgat
    15301 ttcgctgctg gtgggcgcag gcctggggct ggtgggcgtg ctgtttcagc aagtgctgcg
    15361 taatccgctg gcggaaccga cgacacttgg cgttgctaca ggcgcgcaac tgggaattac
    15421 cgtcaccaca ctttgggcga tcccaggtgc tatggcgagc cagtttgccg cactggtggg
    15481 cgcttgtgtc gttggcttaa tcgtctttgg cgtcgcgtgg gggaaacggc tttcgccggt
    15541 tacgcttatt ctcgcggggc tggtggtgag cctttattgc ggggcaatta atcagctact
    15601 ggttatcttc catcacgacc agctgcaaag catgttcctg tggagcaccg gaacgctgac
    15661 gcaaaccgac tggggcggcg ttgagcgttt atggccgcag ttgctgggcg gcgtgatgtt
    15721 gacgttattg ctacttcgcc cgttaaccct gatggggctt gatgatggcg tggcgcgcaa
    15781 tctcgggctg gccttgtcgc tcgcgcgtct ggcagcgctg tcgctggcga ttgtcatcag
    15841 tgcgctgctg gtgaacgctg tggggattat cggctttatc gggttgttcg cgccgctgct
    15901 ggcaaaaatg ctgggggcgc ggcgtctgct gccacgactg atgctggcgt cgctgattgg
    15961 ggcgctgatc ctctggcttt ccgatcaaat catcctctgg ctgactcgcg tgtggatgga
    16021 agtgtccacc ggttcggtca ctgcgttgat cggtgcgccg ctgctactgt ggctgttgcc
    16081 gcgtttacgc agcattagcg cgccggatat gaaggtcaac gatcgtgtcg cggctgaacg
    16141 ccaacatgtg ctggcgtttg cccttgctgg cggtgtgctg ctgataattg ctgtggtggt
    16201 ggcgctgtcg tttggtcgtg atgcgcacgg ctggacgtgg gcgagcgggg cgctgctcga
    16261 tgatttaatg ccctggcgct ggccgcgaat tatggcggcg ctgtttgcgg gcgtcatgct
    16321 ggcggtggcg ggctgtatta ttcagcggct gaccggaaac ccgatggcaa gcccggaagt
    16381 gctggggatt agctctggcg cagcgtttgg cgtggtgttg atgctgtttc tggtgccggg
    16441 taatgccttt ggctggttgt tacctgcagg cagtctcggg gcggcggtga cgctgttgat
    16501 cattatgatc gccgccggac gcggtggatt ttccccacac cgcatgttac tggcggggat
    16561 ggcgttaagt accgcgttca ccatgctttt gatgatgttg caggcaagtg gtgacccgcg
    16621 aatggcgcaa gtgctgacct ggatttccgg ttcgacctac aacgcgaccg atgcgcaggt
    16681 ctggcgcacc ggaattgtga tggtgatttt gctggcgatt accccgctgt gccgccgctg
    16741 gctgaccatt ttaccgctgg gtggtgatac cgcccgagcc gtaggaatgg cgctaacgcc
    16801 gacgcgaatt gcgctgctgc tgttagcggc ttgcctgacg gcgaccgcga cgatgactat
    16861 tggaccgttg agttttgttg gtttaatggc accgcatatt gcgcggatga tgggctttcg
    16921 acggacgatg ccgcacatcg taatttcggc gctggtgggt ggtttactgc tggtgttcgc
    16981 tgactggtgt gggcggatgg tgctgtttcc attccagatc ccggcggggc tgctgtcaac
    17041 ctttatcggc gcgccatatt ttatctattt gttgagaaag cagagccgtt aattgaatgc
    17101 ctcgcccggt gggcgaggca aaatcatcac aacttcgcaa acacccgacg tgcagcatcg
    17161 atggtgttat tgatatcttc catgctgtgc gccacggaca taaagcccgc ttcaaacgct
    17221 gacggtgcca ggtaaacacc ttcatccagc atcatatgga agaaacgctt aaagcgttcc
    17281 acgtcacagg ccatcacatc ctgatagcac gtcacggact cggcgtcggt aaagaaaata
    17341 ccgaacatgc cgccaacgtg gttaacgacc agcggaattc cggcttcttc tgccgcttcc
    17401 agcagacctt ctgccagacg tgatgtcagc tcatccaacg tttcgtgaac gcccggctgc
    17461 gcgacttcat tcagacaggc gaaaccggct gccatcgcaa ttgggttacc ggaaagcgta
    17521 cccgcctgat agaccggacc cgtcggggcc agcgcatcca ttacatcacg acgaccaccg
    17581 aatgcgccta ccggcattcc accgccgatg atttttccga ggcaggttaa atctggcact
    17641 acgccgtaat aatcctgtgc accagccagt gccacgcgga agccagtcat tacttcatcg
    17701 atgatcagca atgcgccaaa ttcgtcgcac agcgcgcgca gacctggcag gaactctggc
    17761 agcggcggaa cacagttcat attgcctgcc accggctcaa cgataataca ggcaatctct
    17821 tgcgggtatt gctcaaacgc ggcgcgtaca gaagccagat cgttataagt acaggttaag
    17881 gtatgtttgg cgaaatctgc cggaacaccc ggcgagtttg gctggcctaa cgtgagtgcg
    17941 ccagaaccgg ctttcaccag caggcagtca gcgtgaccgt ggtaacaacc ttcaaattta
    18001 atgattttgt cgcgaccggt aaaaccacgg gccaggcgga tggcgctcat ggtcgcttca
    18061 gtgccggagt tcaccatgcg caccatatcc atggtcggga ccagttcggt caccagttgc
    18121 gccattttca cttccatttc ggttggtgca ccaaagctta aaccacgctc ggcggcttca
    18181 atcacggcat tgcggattgc cggatggtta tggcccagca ccatcggccc ccaggaaccg
    18241 acataatcga tataggcttt gccatcaaca tcgtacagat aagcgccgtc cgctttttcg
    18301 ataaacagtg gagtgccgcc cacgccagta aaggcgcgaa caggggagtt cacaccgcca
    18361 gggatcagct cgcgcgctgc gctgtaaaga ttttcagact tactcatgga gggttcctga
    18421 ttcgtagaaa aagtgaatgg ctgctattct atgttattca taacaagtta aatactcgtc
    18481 aaacatcagg ctgcttgtac tggtgcaatc ctgatttctt agagtataaa agttttgtgc
    18541 atttgaaaca ttacgctttg caaaggattt tcatggaacg tgcgagtaaa atgccgtcat
    18601 cttatttgta tgaccaataa gtgatcattg gatgaaaact gatactccct ctttagaaac
    18661 accgcaggcc gcgcgcctgc gacgcagaca actgattcgc caacttcttg agcgcgataa
    18721 aaccccgtta gccattttgt ttatggcggc agtcgtcggc acgcttgtcg ggctggctgc
    18781 ggttgctttt gacaaaggtg tcgcctggtt gcagaaccaa cgtatggggg cgctggtaca
    18841 tactgctgat aattatccgc tactgttaac tgtcgctttt ctctgttcgg cggtgctggc
    18901 gatgttcggc tactttttgg tgcgcaaata cgcgcccgaa gcagggggat cggggatccc
    18961 ggaaattgaa ggggcgctgg aagatcaacg tcctgttcgc tggtggcgtg tattgccggt
    19021 gaaattcttt ggcgggctgg ggacactcgg cggaggcatg gtgctagggc gcgaaggacc
    19081 aaccgtgcag atcggcggca atatcggtcg catggtgctt gatattttcc gcctgaaagg
    19141 tgatgaagct cgccatacgc tgctggcaac cggtgctgct gcggggctgg ctgcggcctt
    19201 taacgcgccg ctggcgggta ttttgtttat tatcgaagag atgcgtccgc agtttcgcta
    19261 tacgttaatt tcgattaaag cggtatttat tggcgtcatt atgtcgacca ttatgtaccg
    19321 gatttttaat catgaagttg cgttgattga cgtcggtaaa ctttctgatg cgccgcttaa
    19381 tacgctgtgg ctttatctga tcctcggtat tatttttggc attttcggcc ctatttttaa
    19441 taaatgggtg ctggggatgc aggatttgct gcaccgtgtg cacggcggca atattaccaa
    19501 atgggtgcta atgggcggtg cgattggcgg tctgtgtgga ttgctggggt ttgtggcacc
    19561 agcaacgtcg ggcggcggtt ttaacctgat tcctatcgct accgcgggga atttcagcat
    19621 gggaatgctg gtgtttatct tcgtcgcgcg ggtcattacc accttactct gcttctcttc
    19681 cggcgcgccg ggcggtattt ttgccccgat gctggcgctg ggtactgtgc tgggaaccgc
    19741 tttcggaatg gttgccgttg agctgtttcc gcaatatcac cttgaggcgg gaacgtttgc
    19801 tattgccgga atgggggcgt tactggcggc atctattcgc gcgccgttaa cggggatcat
    19861 tctggttctg gagatgaccg ataactacca gctcattttg ccaatgatta ttaccggtct
    19921 tggcgcaaca ctattagcgc aatttaccgg cgggaaaccg ctatactcgg cgattcttgc
    19981 gcgcacgctg gcaaaacagg aagctgagca actggcgcga agcaaggccg catcagccag
    20041 cgagaatact tgaacgaaat accagggtat tagataatgg cgattattat tgggttagaa
    20101 tttgcccaat tgccgatgtc gtttggagca aaatatgagt gatgacgtag cactgccgct
    20161 ggagtttacc gacgcagcag ccaacaaagt taaaagcctg atcgctgacg aagataaccc
    20221 gaatctgaaa ttacgcgtgt atatcaccgg tggcggttgc agcggcttcc agtatggttt
    20281 cacctttgat gatcaggtga acgaaggcga tatgaccatc gaaaaacagg gcgttggcct
    20341 ggtggttgat ccgatgagcc tgcaatatct ggtcggcggt tccgttgact ataccgaagg
    20401 actggaaggt tctcgtttca tcgtgaccaa cccgaacgcg aaaagcacct gcggttgcgg
    20461 ttcttccttt agcatctaat ctgttgttca tgtgccggat aaactatccg gcacatcttc
    20521 tctcttcaac gcccattctc atccagcgca aacgtcggta gcttaagatg ccaacgaata
    20581 gccgccagcc gaatcaatag cgtcacgacc atgcccatca tactggctgt ttccagtggt
    20641 acggaaaatg tgtaataagc cgtagcgtgg acaataccgc cgataataca ggcagttgcg
    20701 tagatttctg tacgtaaaat catggggatc tcgcgggcca gtacatcacg aataatcccg
    20761 ccgccaacgc cagtaatgac gcccatacaa accgcgatta acggaccggc ttccgcatta
    20821 aaggctttat tcacgccaat gccgacaaac accgccagac caacggcgtc cagcaccggc
    20881 aacatccatt ttggtaagcg tcttggctgg cgcaccagca cgatggtcag catgctggtg
    20941 accattgcaa cgaccagatc ggtgggatct ttcacccaaa ataccgggcc gtgatccagc
    21001 gccatgtcgc gaattgttcc gccgcctact gcggttacca cgcccagtac cagaacacca
    21061 aaagggtcca tacgcaattt tccggctaac aaaacgccgg agatggcaaa taccgctgtg
    21121 ccgactatat ccagccaata gacgagcatt gttcaatccc cactgagcac ctgaaaaggt
    21181 caggcactaa tctacctgtg aaagcgcatt acagagctgt tgtgctgcga ggataatacg
    21241 tgggcttgca cgttcaaacc agtcactcgt gagaggaata acgggaattt tgagctgttc
    21301 accccagtat tgtttgattt taggaatttg gtccggtccg cctgtaatga caatcgcctg
    21361 tggcgagcgt gctaacacct gttcgcggct aacttgcggc cagggaaccc ggctgtcttt
    21421 aaagatgttt tctccgccac aaacttcgag tacctggttc tgaatcgact cttttccact
    21481 ggtaaatggc ggattaatgc cgaattgcag aaaaacacgt tttttaggtt tatcagcata
    21541 ttgcgctttc aattgcgcgt actgatccag cagggattgc gcggcttgtt cggccttgtc
    21601 tggttgcgga ctccaggggg ccagttgacg taacgcattg gcaatttgtt caatgcttgt
    21661 cgcatcgacc cacatcactt ttattcccag cgaagccagc tggtcaacct gccgctcggc
    21721 attacctcca cgccaggcaa tcaccagatc gggcttcagc gcgacaatgc gttccagatt
    21781 catcccctgc caggtggaaa cctgctcaat cttttgcgct tgtggaggat agtcggaata
    21841 gctgctgacc ccaaccggcg tgatcccggc agcaaaggca agttcagtgt tggcgggaga
    21901 aagcgtgatg acgcgcggcg cggcgttgag ccacagtggc gcaagaaaag acagggcgac
    21961 cagcgccctg aacagtgact tagccatgtg caagtttctg caccagtgac tcaaccatca
    22021 ggctggactg tttagcggca acagccagga actcatcgaa gctaagatga gactgttgat
    22081 cggccacgtc ggagatggcg cgtacgacaa caaacgggac gttgaaattg tggcagacat
    22141 gggcgattgc cgtcgcttcc atctctacag caatggcctg tgggaagttg tggcggattt
    22201 tcgccagacc aacagaaccg ttgatgaaag cgtcgccgct aacaatcagg ccacgtacag
    22261 cgttaagatt cagttcggca atgcaggcct cagcggcagc gatcagttta tcgtcagctt
    22321 taaagcctgc cggacagcct ggtaactgac cgtattcata accaaatgcc gtgacatccg
    22381 cgtcgtgata acgtgcttcg tccgagacaa cgatatcgcc cactttcaac gttggtgcca
    22441 ggccaccggc agaaccggtg ttaataatca catctggctt gcagtgttcc aacagcaaag
    22501 tggcacccag cgccgcagcg actttaccga tgcccgattt cagaagcgca acctcggttc
    22561 cattcagttg gccggtatag atttcgcaac cgccgagact gatagtttga cgtttttcga
    22621 ttttgtcacg cagcagcgta acttcttctt ccattgcacc aatgatgccg attttcatag
    22681 atttactcgc gataagcccg atttgaaggc atagtttacc atgtgcttac ggggaagcgt
    22741 atttctcacg cgggagagga catggcacag attgatttcc gaaaaaaaat aaactggcat
    22801 cgtcgttacc gttcaccgca gggcgttaaa accgaacatg agatcctgcg gatcttcgag
    22861 agcgatcgcg ggcgtatcat caactctccg gcaattcgtc gtctgcaaca aaagacccag
    22921 gtttttccac tggagcgcaa tgccgccgtg cgcacgcgtc ttacccactc gatggaagtc
    22981 cagcaggtgg ggcgctacat cgccaaagaa attctaagcc gtctgaaaga gcttaaatta
    23041 ctggaagcat acggcctgga tgaactgacc ggcccctttg aaagcattgt tgagatgtca
    23101 tgcctgatgc acgatatcgg caatccgccg tttggtcatt ttggcgaagc ggcgataaat
    23161 gactggtttc gccagcgttt atacccggaa gatgccgaaa gccagcctct gactgacgat
    23221 cgctgcagcg tggcagcact acgtttacgg gacggggaag aaccgcttaa cgagctgcgg
    23281 cgcaagattc gtcaggactt atgttatttt gaggggaatg cacaaggcat tcgtctggtg
    23341 catacattga tgcggatgaa tctcacctgg gcacaggttg gcggtatttt aaaatatacc
    23401 cgtccggcgt ggtggcgtgg cgaaacgcct gagacacatc actatttaat gaaaaagccg
    23461 ggttattatc tttctgaaga agcctatatt gcccggttgc gtaaagaact taatttggcg
    23521 ctttacagtc gttttccatt aacgtggatt atggaagctg ccgacgatat ctcctattgt
    23581 gtggcagacc ttgaagatgc agtagagaaa agaatattta ccgttgagca gctttatcat
    23641 catttgcacg aagcgtgggg ccagcatgag aaaggttcgc tcttttcgct ggtggttgaa
    23701 aatgcctggg aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt
    23761 atgtatttac gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt
    23821 gataatctgc ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc
    23881 gaatgcagcg atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat
    23941 ccagatgtcg agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt
    24001 tatcgtcctt tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg
    24061 gtgaaacgtt tccctattga atcgcgctta ttccacaaac tctcgacgcg ccatcggctg
    24121 gcctatgtcg aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa
    24181 tattattacc gttgccgcct gttgcaggat tatatcagcg gtatgaccga cctctatgcg
    24241 tgggatgaat accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa
    24301 caataaattt ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc
    24361 tgaagaacac agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc
    24421 acattagcac tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca
    24481 acggcggctg agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg
    24541 ctcgaaaagg tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat
    24601 acgccgcgta tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag
    24661 gaaggttcac cgttccagag ctctccgttc tgccaggggg gccagggcgg taatggcggc
    24721 ggccagcaac agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc
    24781 tatgtcgtca ccaacaacca cgttgttgat aacgcgacgg tgattaaagt ccagctgagc
    24841 gatggtcgta agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg
    24901 attcagatcc agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg
    24961 cgcgtgggtg attacaccgt agcgattggt aacccgtttg gcctgggaga gacggtaact
    25021 tccgggattg tctctgcgct ggggcgtagc ggcctgaatg ccgaaaacta cgaaaacttc
    25081 atccagaccg atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac
    25141 ggcgaactga tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc
    25201 ggttttgcta tcccgagcaa catggtgaaa aacctgacct cgcagatggt ggaatacggc
    25261 caggtgaaac gcggtgagct gggtattatg gggactgagc tgaattccga actggcgaaa
    25321 gcgatgaaag ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc
    25381 gctgcaaaag cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccaatc
    25441 agcagctttg ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc
    25501 ctgggcttgc tgcgcgacgg taagcaggtc aacgtgaatc tggaactgca gcagagcagc
    25561 cagaatcagg ttgattccag ctccatcttc aacggcattg aaggtgctga gatgagcaac
    25621 aaaggcaaag atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag
    25681 atcggcctga agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc
    25741 gctgaactgc gtaaagttct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc
    25801 ggcgacagct ccatctacct gttaatgcag taatctccct caaccccttc ctgaaaacgg
    25861 gaaggggttc tccttacaat ctgtgaactt caccacaact ccatacatct tcatcatcct
    25921 ttaggcattt gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg
    25981 aggacttatg gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac
    26041 catgcgcatc atcgatacca atatcaacgt aatggatgcc cgtgggcgaa ttatcggcag
    26101 cggcgatcgt gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg
    26161 acgagtcgtc gatatcgatg acgcggtggc acgtcatctg cacggtgtgc ggcaagggat
    26221 taatctaccg ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc
    26281 agagaatctg cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca
    26341 gtcgcggttg atgcacttgt tggctcagga tagccgtttg cgggaggaac tggtgatgaa
    26401 cctgattcag gcagaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat
    26461 cgatctcaat caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggtgt
    26521 ggacagcgca atggcggagt tacaacaact gcaaaacgcg ctgactacgc ccgagcgtaa
    26581 taatctggtg gcgattgtct cgctaaccga aatggtggtg ttgaaaccgg cgttgaactc
    26641 ttttgggcgc tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat
    26701 gaaagagtac ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg
    26761 cagtattgcc cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat
    26821 gccagaaagt cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt
    26881 gcgtggcgac tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga
    26941 caataacggc ttgctgcgac gaacgctggc ggcgtggttt cgtcacaatg tgcaaccgct
    27001 ggcaacgtca aaggcgttgt ttattcatcg taatactctg gagtatcggc ttaatcgtat
    27061 atcggaactg accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc
    27121 gttgcagctg gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt
    27181 cccctcagcc cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta
    27241 cgcgttagtt tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc
    27301 aggtgacgta agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg
    27361 cagatttgat caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct
    27421 ttataaccct gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac
    27481 ttaacgctct tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg
    27541 ttgttggctt cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga
    27601 cttttcagat tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt
    27661 agcatttggc ctggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac
    27721 ttctatcatg gcgtaaaaat caaaaaatta cctgctttat tgtggcgata ttccatggga
    27781 tggattaaca catttatctt cgttttgcga attacatgac agtccgaaac aagatgaaaa
    27841 agtatttaaa aaagggggga agaagaatga gtaggacgaa ttattctgag ccagaaagca
    27901 tacgggcagc atcatgctgc ccgtatatgt actcttaatc gatggtacgc agcagttcgt
    27961 taatgccgac tttgccgcga gttttcgcgt caactttctt aacgataacc gcacagtaga
    28021 ggctgtattt gccatctttc gacggcagat tacctgaaac aaccacagac cccgccggaa
    28081 cgcgaccgta gtggatttcg ccggtttcac ggtcgtaaat acgggtgctc tgaccaatgt
    28141 atacgcccat agagatgacg gaaccttctt cgacaatcac cccttcaacc acttcggagc
    28201 gtgcgccaat gaagcagtta tcttcaataa tggtcgggtt agcctgcagc ggctccagca
    28261 cgccgccgat gcccacgcca ccggaaaggt ggacgttttt accaatctgc gcacaagaac
    28321 cgacggtcgc ccaggtatca accatcgtgc cttcatcaac atatgcgccg atgttgacgt
    28381 aagacggcat cagcacggtg ttacgggcaa taaatgcacc ctgacgtacc gccgctggtg
    28441 gcacaacgcg gaagccttct ttctgaaaac gcgcttcgtc gtagtcggcg aatttcatcg
    28501 gcactttatc gaagtagcgg ctttctgccc cttcgatcac ctgattatca ttaatacgga
    28561 aagagagcag caccgctttt ttcaaccact gatgcgtcac ccactgaccg tcaatttttt
    28621 ccgctacacg cagtgcgccg gaatccagca gggcgatcac ctgatttacc gcttcgcggg
    28681 ttacggtgtc tgcattggct ggcgtgatct cggcacggcg ttcaaaagcg gtttcaataa
    28741 tgttctgtaa ctgctgcatt gttaaactct tttcatatca gtaaacacat caccctttat
    28801 cgtttggatt gagggcctct gtcaaccgct gatgcacttc ctgctgcaac tcgttattaa
    28861 gtgcacgccg gtcagcggtg gcaattatga ataaatcttc tactcgctcg ccaatggttg
    28921 taattcgggc accatgaagc gaaattccca gatcggcaaa aattttcccg actctcgcca
    28981 gcagtcctgg ctggtcgagc gcgataagct caaggaacga tttgcggtcg gtatgggtcg
    29041 gcaaaaacgt tacttcggtt tcaacagtaa aatggcgtaa tttggcgggt tggcgacggg
    29101 gctgcggtgg ctgccagcta ctttgcgtca gtacttgctc cagaccaaac cgaataacct
    29161 catgacgatc tgcggacagc gggctgccat cgggttccag cacgataaag gtatccatcg
    29221 ccataccgtc gcgagtggtg aaaatttgtg cgtcgtgaac acttaaattg cggcggtcta
    29281 attcggcaca gacggcggca aacagatagg ggcggtccgg gctccagata aaaatctcgg
    29341 tgcctccacg cgtagcctgc gggctaagca ataccagcgg tttgcttaaa tcatgctgta
    29401 ataaatggcg ggcatgccag gccagttgat ttgggctatg gcggacaaaa tagttagcac
    29461 gacagcgtga ccaaatttgg tgcagcgcct cttcgtcgat gttatccatg cgcagtagtg
    29521 ccagtgcctg gagttggtga tggcgaaccc gttcgcgcat atccggcgtg ttttgcattc
    29581 cgcgtcgtag ctgcttttcg gtggcaaaat agagttcgcg caacagactt tgcttccagc
    29641 tattccacag cgtttcgttg gtggcgcaaa tgtcagccac tgtcaggcat accagatagc
    29701 gcagacgatt ttccgtttga acttcttcgg caaactgctt gatgacttca gggtcctgaa
    29761 tatcgcggcg ttgtgctgtc accgacatca gcaagtgctg gcgaaccagc caggcaacca
    29821 gctgtgtttc gcgtgagttc agcccgtgga gttctgcaaa atgcactaca tcctgagcac
    29881 cgagaatgga gtggtcgccg ccgcgtcctt tggcgatatc gtgaaacagc gcggcgatga
    29941 aaatcagctc agttgacggc aggcgcggcc agacgtccac acacaacgga tggcgctggc
    30001 gcgtttcttc actggcaaaa ctctccagtt tcagcatcac gcggatagta tgttcatcca
    30061 ccgtgtaggc gtggaacaga tcaaactgca tctgcccgac gatatgcgac cattgcggca
    30121 tatacgcgcc gagcacgcta tggcgatgca ttggcaatag cccgcgccgc accgctccgg
    30181 ggtgacgcag aatgctcaaa aacagttttc gtgcttccgg aatattacac agcggttgtt
    30241 gcagatggcg acgggcatgg cgtaactggc gcagcgtggt ggagtaaatg ccggtgatcg
    30301 cactgttgcg caccatggtg tagaacatac gcaagatggc ttccggctgg cgcataaata
    30361 gtgtttcatc acgcaggtcg attagcgtac cgcgtagctg aaactcatcg tcgattggac
    30421 gtggtttttc gtcggcggga agggcgagga tggcttcatc gaacagttgc agcagcatct
    30481 ggttgagttc actgacgcgg cgtgtaacgc ggaagtaatc cttcatcatc cgctcgaccg
    30541 gttcgttgcc ttcaccactg taattcagac gctgagcgac gctaagttgg cgatcgaata
    30601 acagacgatt atcgtaacgg ctgacgacca gatgcagggc aaagcgaata cgccacagta
    30661 tatgcagaca ttcgtttaat tccgcccgct ccgctgaggt taagaagcca aacccgacca
    30721 tttcatccag cgatgttgcg ccaaaatgac ggcgggccac ccattgcaga gtgtgaatat
    30781 cgcgcaagcc gccagggcta cttttgatgt ctggttcaag gttgtagctg gtgccatggt
    30841 aacgctgatg gcgctggttc tgttcttcaa ctttcgccgc gtagaacttg tcggaaggcc
    30901 agaatccttc gctaaaaata tgtttttgca gttcgaggaa gagcgcaaca tcgccaatta
    30961 ataagcggga ttcgattaaa ttggtggcga cggttaaatc cgataaccct tccagcatgc
    31021 actcttcaag cgtgcgcaca ctatgaccga cttccagctt tacatcccag agcagcgtta
    31081 acagctcgcc cactttttgc gcctgatcgt ccgggagctt tttacggctt aaaatcagta
    31141 aatcgatgtc agaaagtgga tgcagctcgc cacgaccgta gccaccgacg gcgaccaatg
    31201 ccaggtcggc aatctggctg aatcccgctt caatccataa tcgttgcagg agctggtcga
    31261 taaactcggt gcgcgcctca atcaactgtt ctgcagagat cccattgtca aaggcatcac
    31321 ccagccaacg ctggaaagta tcgatatggg cttttatccc accgacggtt aattcatcac
    31381 ggggccagac gcatggattt tgcggttgac cgggcagggt ggggagagcg gtgtttgcgt
    31441 actgttctgg aagggtattc attgtgcgcc acccataaga ttaaattatc gcattaaaaa
    31501 agccggcatt cgccggcttc atcttattcg tcgtgcgaga ttatcgccgg gatggtgtca
    31561 tccttgcgta gcgtcaaaat ttcgcagccg ttatcagtca ccacaatagt atgctcatat
    31621 tgtgcagaca agctgcgatc tttggttttt accgtccagc catctttcat ggtgcggatc
    31681 tcttttttac ccgcgttgac cattggctca atggtgaacg tcatccccgg tttcagcacg
    31741 acgttggttt cacgggagtc atagtgcagc acctgcggtt cttcatggaa gccgcgacca
    31801 ataccgtgtc cacaatattc acgaacgacg gagaagcctt ctgcttcgac aaacttctga
    31861 atcgccgcac cgatttcgcg caggttaatg cctggtttga ccatgcgcag cgccaggtac
    31921 aggctttctt gcgtgatgcg gcacagacgt tcgcccatga tggtcggctt accgacgata
    31981 aacattttcg aggtatcgcc gtggaaacca tctttgatta cggtgacatc aatgttaacg
    32041 atatcgccat ctttcagcag cttagcatcg tccgggatac cgtggcacac cacttcatta
    32101 atagagatgc aaacggattt cggatagccg tgatagccga ggcaggcaga aaccgcgtgt
    32161 tgttcattaa caatgtaatc attacagatg cgatccagct cgccggtgct gacgcccggt
    32221 ttaacatacg gttcgatcat ctccagcact tcggcagcca gtcggccagc gacgcgcatt
    32281 ttttcgatat cttctggggt cttgattgag atagccatta attctgtcca tcagcgtcgg
    32341 tgataccgac aatatatatg taagtgccgt caatggtatc acacccgggc aaattgagaa
    32401 tcattctgaa tttcgccaaa cgtgccactg aaggttttct ataatagaaa attcgacatc
    32461 tgatgctgta cacagcgcca acaattattg gtgtccatga cgtatttgtg gtataaagcg
    32521 cgccggactt ccgatccatt tcgtatacac agactggacg gaagcgacaa tctcactttg
    32581 tgtaacaaca cacacgtatc ggcacatatt ccggggtgcc ctttggggtc ggtaatatgg
    32641 gatacgtgga ggcataaccc caacttttat atagaggttt taatcatggc aactgtttcc
    32701 atgcgcgaca tgctcaaggc tggtgttcac ttcggtcacc agacccgtta ctggaacccg
    32761 aaaatgaagc cgttcatctt cggtgcgcgt aacaaagttc acatcatcaa ccttgagaaa
    32821 actgtaccga tgttcaacga agctctggct gaactgaaca agattgcttc tcgcaaaggt
    32881 aaaatccttt tcgttggtac taaacgcgct gcaagcgaag cggtgaaaga cgctgctctg
    32941 agctgcgacc agttcttcgt gaaccatcgc tggctgggcg gtatgctgac taactggaaa
    33001 accgttcgtc agtccatcaa acgtctgaaa gacctggaaa ctcagtctca ggacggtact
    33061 ttcgaaaagc tgaccaagaa agaagcgctg atgcgcactc gtgagctgga gaaactggaa
    33121 aacagcctgg gcggtatcaa agacatgggc ggtctgccgg acgctctgtt tgtaatcgat
    33181 gctgaccacg aacacattgc tatcaaagaa gcaaacaacc tgggtattcc ggtatttgct
    33241 atcgttgata ccaactctga tccggacggt gttgacttcg ttatcccggg taacgacgac
    33301 gcaatccgtg ctgtgaccct gtacctgggc gctgttgctg caaccgtacg tgaaggccgt
    33361 tctcaggatc tggcttccca ggcggaagaa agcttcgtag aagctgagta ataaggcttg
    33421 ataactcccc caaaatagtt cgagttgcag aaaggcggca agctcgagaa ttcccgggag
    33481 cttacatcag taagtgaccg ggatgagcga gcgaagataa cgcatctgcg gcgcgaaata
    33541 tgaaggggga gagcccttat agaccaggta gtacacgttt ggttaggggg cctgcatatg
    33601 gccccctttt tcacttttat atctgtgcgg tttaatgccg ggcagatcac atctccgagg
    33661 attttagaat ggctgaaatt accgcatccc tggtaaaaga gctgcgtgag cgtactggcg
    33721 caggcatgat ggattgcaaa aaagcactga ctgaagctaa cggcgacatc gagctggcaa
    33781 tcgaaaacat gcgtaagtcc ggtgctatta aagcagcgaa aaaagcaggc aacgttgctg
    33841 ctgacggcgt gatcaaaacc aaaatcgacg gcaactacgg catcattctg gaagttaact
    33901 gccagactga cttcgttgca aaagacgctg gtttccaggc gttcgcagac aaagttctgg
    33961 acgcagctgt tgctggcaaa atcactgacg ttgaagttct gaaagcacag ttcgaagaag
    34021 aacgtgttgc gctggtagcg aaaattggtg aaaacatcaa cattcgccgc gttgctgcgc
    34081 tggaaggcga cgttctgggt tcttatcagc acggtgcgcg tatcggcgtt ctggttgctg
    34141 ctaaaggcgc tgacgaagag ctggttaaac acatcgctat gcacgttgct gcaagcaagc
    34201 cagaattcat caaaccagaa gacgtatccg ctgaagtggt agaaaaagaa taccaggtac
    34261 agctggatat cgcgatgcag tctggtaagc cgaaagaaat cgcagagaaa atggttgaag
    34321 gccgcatgaa gaaattcacc ggcgaagttt ctctgaccgg tcagccgttc gttatggaac
    34381 caagcaaaac tgttggtcag ctgctgaaag agcataacgc tgaagtgact ggcttcatcc
    34441 gcttcgaagt gggtgaaggc atcgagaaag ttgagactga ctttgcagca gaagttgctg
    34501 cgatgtccaa gcagtcttaa ttatcaaaaa ggagccgcct gagggcggct tctttttgtg
    34561 cccatcttgt aaattcagct aacccttgtg gggctgcgct gaaaagcgac gtacaatgtc
    34621 gctagtatta attcatttca atcgttgaca gtctcaggaa agaaacatgg ctaccaatgc
    34681 aaaacccgtc tataaacgca ttctgcttaa gttgagtggc gaagctctgc agggcactga
    34741 aggcttcggt attgatgcaa gcatactgga tcgtatggct caggaaatca aagaactggt
    34801 tgaactgggt attcaggttg gtgtggtgat tggtgggggt aacctgttcc gtggcgctgg
    34861 tctggcgaaa gcgggtatga accgcgttgt gggcgaccac atggggatgc tggcgaccgt
    34921 aatgaacggc ctggcaatgc gtgatgcact gcaccgcgcc tatgtgaacg ctcgtctgat
    34981 gtccgctatt ccattgaatg gcgtgtgcga cagctacagc tgggcagaag ctatcagcct
    35041 gttgcgcaac aaccgtgtgg tgatcctctc cgccggtaca ggtaacccgt tctttaccac
    35101 cgactcagca gcttgcctgc gtggtatcga aattgaagcc gatgtggtgc tgaaagcaac
    35161 caaagttgac ggcgtgttta ctgctgatcc ggcgaaagat ccaactgcaa ccatgtacga
    35221 gcaactgact tacagcgaag tgctggaaaa agagctgaaa gtcatggacc tggcggcctt
    35281 cacgctggct cgtgaccata aattaccgat tcgtgttttc aacatgaaca aaccgggtgc
    35341 gctgcgccgt gtggtaatgg gtgaaaaaga agggacttta atcacggaat aattcccgtg
    35401 atggataaat aagggtaaga ttccgcgtaa gtatcgcggg ggatcttacc cgtaatatgt
    35461 ttaatcaggg ctatacttag cacacttcca ctgtgtgtga ctgtctggtc tgactgagac
    35521 aagttttcaa ggattcgtaa cgtgattagc gatatcagaa aagatgctga agtacgcatg
    35581 gacaaatgcg tagaagcgtt caaaacccaa atcagcaaaa tacgcacggg tcgtgcttct
    35641 cccagcctgc tggatggcat tgtcgtggaa tattacggca cgccgacgcc gctgcgtcag
    35701 ctggcaagcg taacggtaga agattcccgt actctgaaaa tcaacgtgtt tgatcgttca
    35761 atgtctccgg ccgttgaaaa agcgattatg gcgtccgatc ttggcctgaa cccgaactct
    35821 gcgggtagcg acatccgtgt tccgctgccg ccgctgacgg aagaacgtcg taaagatctg
    35881 accaaaatcg ttcgtggtga agcagaacaa gcgcgtgttg cagtacgtaa cgtgcgtcgt
    35941 gacgcgaacg acaaagtgaa agcactgttg aaagataaag agatcagcga agacgacgat
    36001 cgccgttctc aggacgatgt acagaaactg actgatgctg caatcaagaa aattgaagcg
    36061 gcgctggcag acaaagaagc agaactgatg cagttctgat ttcttgaacg acaaaaacgc
    36121 cgctcagtag atccttgcgg atcggctggc ggcgttttgc tttttattct gtctcaactc
    36181 tggatgtttc atgaagcaac tcaccattct gggctcgacc ggctcgattg gttgcagcac
    36241 gctggacgtg gtgcgccata atcccgaaca cttccgcgta gttgcgctgg tggcaggcaa
    36301 aaatgtcact cgcatggtag aacagtgcct ggaattctct ccccgctatg ccgtaatgga
    36361 cgatgaagcg agtgcgaaac ttcttaaaac gatgctacag caacagggta gctgcaccga
    36421 agtcttaagt gggcaacaag ccgcttgcga tatggcagcg cttgaggatg ttgatcaggt
    36481 gatggcagcc attgttggcg ctgctgggct gttacctacg cttgctgcga tccgcgcggg
    36541 taaaaccatt ttgctggcca ataaagaatc actggttacc tgcggacgtc tgtttatgga
    36601 cgccgtaaag cagagcaaag cgcaattgtt accagtcgat agcgaacata acgccatttt
    36661 tcagagttta ccgcaaccta tccagcataa tctgggatac gctgaccttg agcaaaatgg
    36721 cgtggtgtcc attttactta ccgggtctgg tggccctttc cgtgagacgc cattgcgcga
    36781 tttggcaaca atgacgcccg atcaagcctg ccgtcatccg aactggtcga tgggacgtaa
    36841 aatttctgtc gattcggcta ccatgatgaa caaaggtctg gaatacattg aagcgcgttg
    36901 gctgtttaac gccagcgcca gccagatgga ggtgctgatt cacccgcagt cagtgattca
    36961 ctcaatggtg cgctatcagg acggcagtgt tctggcgcag ctgggggaac cggatatgcg
    37021 tacgccaatt gcccacacca tggcatggcc gaatcgcgtg aactctggcg tgaagccgct
    37081 cgatttttgc aaactaagtg cgttgacatt tgccgcaccg gattatgatc gttatccatg
    37141 cctgaaactg gcgatggagg cgttcgaaca aggccaggca gcgactacag cattgaatgc
    37201 cgcaaacgaa atcaccgttg ctgcttttct tgcgcaacaa atccgcttta cggatatcgc
    37261 tgcgttgaat ttatccgtac tggaaaaaat ggatatgcgc gaaccacaat gtgtggacga
    37321 tgtgttatct gttgatgcga acgcgcgtga agtcgccaga aaagaggtga tgcgtctcgc
    37381 aagctgagga taatccggct acagagagtc gcgctatttg ttagcgtagg gcttcagtga
    37441 tatagtctgc gccatctgat cgtaagtagt tggctttata aggtcaggta tgccgtggtt
    37501 ttacacggct tttttttgta taggcttcag tattcctgag taccgtaaac cctgtcaggg
    37561 aataaaaaac gcgtgatgtt gtctgctact caaccactta gcgaaaaatt gccagcgcat
    37621 ggctgccgtc atgttgcgat cattatggac ggcaatggtc gctgggcaaa aaagcaaggg
    37681 aagattcgtg cctttgggca taaagccggg gcaaaatccg tccgccgggc tgtctctttt
    37741 gcggccaaca acggtattga ggcgttaacg ctgtatgcct ttagtagtga aaactggaac
    37801 cgaccagcgc aggaagtcag tgcgttaatg gaactgtttg tgtgggcgct cgatagcgaa
    37861 gtaaaaagtc tgcaccgaca taacgtgcgt ctgcgtatta ttggcgatac cagtcgcttt
    37921 aactcgcgtt tgcaagaacg tattcgtaaa tctgaagcgc taacagccgg gaataccggt
    37981 ctgacgctga atattgcggc gaactacggt ggacgttggg atatagtcca gggagtcagg
    38041 caactggctg aaaaggtgca gcaaggaaac ctgcaaccag atcagataga tgaagagatg
    38101 ctaaaccagc atgtctgtat gcatgaactg gcccctgtag atttagtaat taggactggg
    38161 ggggagcatc gcattagtaa ctttttgctt tggcaaattg cctatgccga actttacttt
    38221 acagatgttc tctggcccga tttcgatgaa caagactttg aaggggcgtt aaatgccttt
    38281 gctaatcgag agcgtcgttt cggcggcacc gagcccggtg atgaaacagc ctgatggggg
    38341 tcgcttttgc tgaagtatcg cctgatatct gcttttgtgt taatacccgt cgtcatcgcg
    38401 gcgttgtttc tgttgccgcc ggtggggttc gccattgtaa cgctggtggt ctgcatgctg
    38461 gcagcgtggg aatggggaca gcttagcggt tttaccactc gttcgcagcg agtatggttg
    38521 gcggtgttat gcgggttatt gttggcgctg atgctttttc tgttgccgga atatcaccga
    38581 aatattcatc aaccgctggt tgaaatctca ctttgggctt cgctgggttg gtggattgtc
    38641 gcgctattgc tggtgctgtt ttacccaggt tccgcagcaa tctggcgtaa ctctaaaaca
    38701 ttgcgcctta tttttggcgt gctaaccatt gttcccttct tctggggcat gctggcgtta
    38761 cgggcctggc actatgacga gaatcattac agtggcgcaa tatggctgct ctatgtcatg
    38821 atcctggtat ggggcgctga ctccggagca tatatgtttg gcaaattgtt tggtaaacat
    38881 aagctggcac cgaaggtttc tccgggtaaa acctggcaag gctttatcgg tggactcgct
    38941 actgcagcgg taatctcatg gggttatggc atgtgggcga atctcgacgt cgctcccgtc
    39001 accttactca tttgctctat tgtcgcagcg ttagcctcag tgctcggcga tctgaccgag
    39061 agtatgttta agcgtgaagc aggaattaag gacagcggtc atttaattcc aggacacggt
    39121 ggtattttag atcgtattga tagcctgacg gctgcggtac cggtctttgc ttgcttgttg
    39181 ttactggtat tcaggacgct ttaacggaag gtaatatgct gagttttctc tgggatttgg
    39241 cttcgttcat cgttgcactg ggtgtactta tcaccgtgca tgaatttggt catttctggg
    39301 ttgcccggcg ttgtggtgtt cgcgttgagc gtttctcaat agggtttggt aaggcgctct
    39361 ggcggcgaac tgataagctc ggcaccgaat atgttatcgc cctgatcccg ttgggcggtt
    39421 atgtcaaaat gctggatgag cgcgcagaac cggtcgttcc ggaactccgc caccatgcct
    39481 tcaataataa atctgtcggc caacgagcgg cgattattgc cgcaggtccg gttgcaaact
    39541 tcatttttgc tatctttgcc tactggctgg tttttattat tggtgtgcct ggcgtacgtc
    39601 cggtggttgg cgaaatagca gccaattcga tagctgcgga agcacaaatt gcaccaggta
    39661 cggaactaaa agccgtagat ggtatcgaaa cgcctgattg ggatgccgtg cgtttgcagt
    39721 tggtcgataa aattggcgat gaaagcacca ccattacagt agcgccattt ggcagcgacc
    39781 aacggcggga tgtaaagctc gatttacgtc actgggcgtt tgagcctgat aaagaagatc
    39841 cggtatcttc gctggggatt cgtcctcgtg ggccgcaaat tgaacctgta ctggaaaatg
    39901 tgcagccaaa ctcggcggca agcaaggcag gtttgcaagc aggcgacagg atcgttaaag
    39961 tcgatggtca gcccttaacg cagtgggtga cctttgtgat gcttgtccgg gataacccgg
    40021 gtaaatcctt agcgttagaa atcgaaaggc agggaagtcc cttgtctttg acattaatcc
    40081 cggagagtaa accgggtaat ggtaaagcga ttggttttgt cggtattgag ccgaaagtca
    40141 ttcctttgcc agatgagtat aaagttgtac gccagtatgg gccgttcaac gccatcgtcg
    40201 aagccacgga caaaacgtgg cagctgatga agctgacggt cagtatgctg ggaaaattga
    40261 tcaccggtga tgtgaaactg aacaacctca gtgggccgat ctctatcgcc aagggggctg
    40321 ggatgacagc ggaactcggg gtagtttatt acctgccgtt tcttgcgctt attagcgtga
    40381 acttagggat aattaacctg tttccgttgc ccgtacttga cggggggcat ctgctgttcc
    40441 ttgcgatcga aaagatcaag ggcggaccgg tatccgagcg ggttcaagac ttttgttatc
    40501 gcattggctc gattctgctg gtgctgttaa tggggcttgc acttttcaat gatttctctc
    40561 ggttatgaga gttagttagg aagaacgcat aataacgatg gcgatgaaaa agttgctcat
    40621 agcgtcgctg ctgtttagca gcgccaccgt atacggtgct gaagggttcg tagtgaaaga
    40681 tattcatttc gaaggccttc agcgtgtcgc cgttggtgcg gccctcctca gtatgccggt
    40741 gcgcacaggc gacacggtta atgatgaaga tatcagtaat accattcgcg ctctgtttgc
    40801 taccggcaac tttgaggatg ttcgcgtcct tcgtgatggt gatacccttc tggttcaggt
    40861 aaaagaacgt ccgaccattg ccagcattac tttctccggt aacaaatcgg tgaaagatga
    40921 catgctgaag caaaacctcg aggcttctgg tgtgcgtgtg ggcgaatccc tcgatcgcac
    40981 caccattgcc gatatcgaga aaggtctgga agacttctac tacagcgtcg gtaaatatag
    41041 cgccagcgta aaagctgtcg tgaccccgct gccgcgcaac cgtgttgacc taaaactggt
    41101 gttccaggaa ggtgtgtcag ctgaaatcca gcaaattaac attgttggta accatgcttt
    41161 caccaccgac gaactgatct ctcatttcca actgcgtgac gaagtgccgt ggtggaacgt
    41221 ggtaggcgat cgtaaatacc agaaacagaa actggcgggc gaccttgaaa ccctgcgcag
    41281 ctactatctg gatcgcggtt atgcccgttt caacatcgac tctacccagg tcagtctgac
    41341 gccagataaa aaaggtattt acgtcacggt gaacatcacc gaaggcgatc agtacaagct
    41401 ttctggcgtt gaagtgagcg gcaaccttgc cgggcactcc gctgaaattg agcagctgac
    41461 taagatcgag ccgggtgagc tgtataacgg caccaaagtg accaagatgg aagatgacat
    41521 caaaaagctt ctcggtcgct atggttatgc ctatccgcgc gtacagtcga tgcctgaaat
    41581 taacgatgcc gacaaaaccg ttaaattacg cgtgaacgtt gatgcgggta accgtttcta
    41641 cgtgcgtaag atccgttttg aaggtaacga tacctcgaaa gatgccgtcc tgcgtcgcga
    41701 aatgcgtcag atggaaggtg catggctggg aagcgatctg gtcgatcagg gtaaggagcg
    41761 tctgaatcgt ctgggcttct ttgaaactgt cgataccgat acccaacgtg ttccgggtag
    41821 cccggaccag gtagatgtcg tctacaaggt aaaagagcgc aacaccggta gcttcaactt
    41881 tggtattggt tacggtactg aaagtggcgt gagcttccag gctggtgtgc agcaggataa
    41941 ctggttaggt acaggttatg ctgttggtat caacgggacc aaaaacgatt accagaccta
    42001 tgctgaactg tcggtaacca acccgtactt cactgtagat ggtgtaagcc tcggtggtcg
    42061 tctcttctat aatgacttcc aggcagatga cgccgacctg tccgactata ccaacaagag
    42121 ttatggtaca gacgtgacgt tgggcttccc gatcaacgaa tataactcgc tgcgtgcagg
    42181 tctgggttat gtacataact ccctgtccaa catgcagcct caggttgcga tgtggcgtta
    42241 tctgtactct atgggtgaac atccgagcac ctctgatcag gataatagct tcaaaacgga
    42301 cgacttcacg ttcaactatg gttggaccta taacaagctt gaccgtggtt acttcccgac
    42361 agatggttca cgtgtcaacc tgaccggtaa agtgaccatt cctggatcgg ataacgaata
    42421 ctacaaagtg acgttagaca cggcgactta tgtgccgatc gatgacgatc acaaatgggt
    42481 tgttctgggg cgtacccgct ggggttatgg tgatggttta ggcggcaaag agatgccgtt
    42541 ctacgagaac ttctatgccg gtggttccag caccgtgcgt ggcttccagt ccaatacaat
    42601 tggtccgaaa gcagtttact tcccgcatca ggccagtaat tatgatccgg actatgatta
    42661 cgaatgtgcg actcaggacg gcgcgaaaga cctgtgtaaa tcggatgatg ctgtaggcgg
    42721 taacgccatg gcggttgcca gcctcgagtt catcaccccg acgccgttta ttagcgataa
    42781 gtatgctaac tcggttcgta cttccttctt ctgggatatg ggtaccgttt gggatacaaa
    42841 ctgggattcc agccaatatt ctggatatcc ggactatagt gatccaagca atatccgtat
    42901 gtctgcgggt atcgcattac aatggatgtc cccattgggg ccgttggtgt tctcctacgc
    42961 ccagccgttc aaaaagtacg atggagacaa ggcagaacag ttccagttta acatcggtaa
    43021 aacctggtaa gtgttctcca caaaggaatg tagtggtagt gtagcgatga ctttaggcga
    43081 tcaatataag atcgccgggc cacgcaaaga actgcaccct ccggtgcaaa tgggatggta
    43141 aggagtttat tgtgaaaaag tggttattag ctgcaggtct cggtttagca ctggcaactt
    43201 ctgctcaggc ggctgacaaa attgcaatcg tcaacatggg cagcctgttc cagcaggtag
    43261 cgcagaaaac cggtgtttct aacacgctgg aaaatgagtt caaaggccgt gccagcgaac
    43321 tgcagcgtat ggaaaccgat ctgcaggcta aaatgaaaaa gctgcagtcc atgaaagcgg
    43381 gcagcgatcg cactaagctg gaaaaagacg tgatggctca gcgccagact tttgctcaga
    43441 aagcgcaggc ttttgagcag gatcgcgcac gtcgttccaa cgaagaacgc ggcaaactgg
    43501 ttactcgtat ccagactgct gtgaaatccg ttgccaacag ccaggatatc gatctggttg
    43561 ttgatgcaaa cgccgttgct tacaacagca gcgatgtaaa agacatcact gccgacgtac
    43621 tgaaacaggt taaataagta atgccttcaa ttcgactggc tgatttagcg cagcagttgg
    43681 atgcagaact acacggtgat ggcgatatcg tcatcaccgg cgttgcgtcc atgcaatctg
    43741 cacaaacagg tcacattacg ttcatggtta acccaaaata ccgtgagcat ttaggcttgt
    43801 gccaggcgtc cgcggttgtc atgacccagg acgatcttcc tttcgcgaaa agtgccgcgc
    43861 tggtagtgaa gaatccctac ctgacttacg cgcgcatggc gcaaatttta gataccacgc
    43921 cgcagcccgc gcagaacatt gcacccagtg cggtgatcga cgcgacggcg aagctgggta
    43981 acaacgtatc gattggcgct aacgcggtga ttgagtccgg cgttgaactg ggcgataacg
    44041 tgattatcgg tgccggttgc ttcgtaggta aaaacagcaa aatcggtgca ggttcgcgtc
    44101 tctgggcgaa cgtaaccatt taccatgaga tccagatcgg tcagaattgc ctgatccagt
    44161 ccggaacagt ggtaggcgca gacggctttg gttatgccaa cgatcgtggt aactgggtga
    44221 agatcccaca gattggtcgc gtaattattg gcgatcgcgt ggagatcggt gcctgcacaa
    44281 ccatcgatcg cggcgcgctg gatgacacta ttattggcaa tggcgtgatc attgataacc
    44341 agtgccagat tgcacataac gtcgtgattg gcgacaatac ggcggttgcc ggtggcgtca
    44401 ttatggcggg cagcctgaaa attggtcgtt actgcatgat cggcggagcc agcgtaatca
    44461 acgggcatat ggaaatatgc gacaaagtga cggttacggg catgggtatg gtgatgcgtc
    44521 ccatcactga accaggcgtc tattcctcag gcattccgct gcaacccaac aaagtctggc
    44581 gcaaaaccgc tgcactggtg atgaacattg atgacatgag caagcgtctg aaatcgcttg
    44641 agcgcaaggt taatcaacaa gactaacgtt ccatcttttg ttcgccaaac tttacggcct
    44701 gtctcattct tacgattgcg gcaggccgtg ttattattgt cgtttcttat attttgacag
    44761 gaagagtatc ttgactacta acactcatac tctgcagatt gaagagattt tagaacttct
    44821 gccgcaccgt ttccctttct tactggtgga tcgcgtgctg gattttgaag aaggtcgttt
    44881 tctgcgcgca gtaaaaaatg tctctgtcaa tgagccattc ttccagggcc atttccctgg
    44941 aaaaccgatt ttcccgggtg tgctgattct ggaagcaatg gcacaggcaa caggtattct
    45001 ggcgtttaaa agcgtaggaa aactggaacc gggtgagctg tactatttcg ctggtattga
    45061 cgaagcgcgc ttcaagcgcc cggtcgtgcc tggcgatcaa atgatcatgg aagtcacttt
    45121 cgaaaaaacg cgccgcggcc tgacccgttt taaaggggtt gctctggtcg acggtaaagt
    45181 agtttgcgaa gcaacgatga tgtgtgctcg tagccgggag gcctgatacg tgattgataa
    45241 atccgccttt gtgcatccaa ccgccattgt ggaagagggc gcgtcgattg gcgcgaacgc
    45301 acacattggt cctttttgta tcgttggacc ccatgtcgaa attggtgagg gtaccgtact
    45361 gaaatctcac gttgtcgtga atggccatac taaaattggc cgcgataatg agatttatca
    45421 gttcgcctcc atcggtgaag ttaaccagga tctgaaatat gctggcgaac cgacccgtgt
    45481 ggaaatcggc gatcgtaacc gcattcgcga aagcgtcacc attcatcgtg gcacagtcca
    45541 gggcggtgga ttgacgaagg tgggcagcga caacttactg atgatcaacg cgcacattgc
    45601 gcacgattgt acggtaggta accgctgtat tctcgccaac aacgcaacgc tggcgggtca
    45661 cgtatcggta gacgacttcg cgatcatcgg cggcatgacc gcagtccatc agttctgcat
    45721 cattggtgcg catgtgatgg ttggcggttg ctccggtgtg gcgcaggacg tccctcctta
    45781 tgtcattgcg cagggtaacc acgcaacgcc gttcggtgtc aatatcgaag ggctgaagcg
    45841 ccgcggattc agccgcgagg cgattaccgc tatccgcaat gcgtataagc tgatttatcg
    45901 cagcggtaaa acgctcgatg aagtgaaacc ggaaattgct gaactggcgg aaacgtatcc
    45961 ggaagtgaaa gcttttaccg atttctttgc acgctcaacg cgcggtctga ttcgttaatg
    46021 actaaacagc gtccattaac gattgccctg gtcgccggag aaacctccgg cgatatcctg
    46081 ggggccggtt taatccgcgc tctgaaagaa cgtgtgccca acgcccgctt tgttggcgtt
    46141 gccgggccac gaatgcaggc tgaaggctgc gaagcctggt acgaaatgga agaactggcg
    46201 gtgatgggca ttgttgaagt gctcggtcgt ctgcgtcgct tactgcatat tcgtgccgat
    46261 ctgacaaagc gttttggtga actaaagcca gatgtttttg ttggcattga tgcgcctgac
    46321 ttcaatatta ccctcgaagg taaccttaaa aagcagggta tcaaaaccat tcattacgtc
    46381 agtccatccg tctgggcgtg gcgacagaaa cgcgttttca aaataggcag agccaccgat
    46441 ctggtgctcg catttctgcc tttcgaaaaa gcgttttatg acaaatacaa tgtaccgtgc
    46501 cgctttatcg gtcataccat ggctgatgcc atgccgttag atccagataa aaatgccgcc
    46561 cgtgatgtgc tggggatccc tcacgatgcc cactgcctgg cgttgctacc agggagccgt
    46621 ggtgcagaag ttgaaatgct tagtgccgat ttcctgaaaa cggcccagct tttgcgccag
    46681 acatatccgg atctcgaaat cgtggtgcca ctggtgaatg ccaaacgccg cgagcagttt
    46741 gaacgcatca aagctgaagt cgcgccagac ctttcagttc atttgctgga tgggatgggc
    46801 cgtgaggcga tggtcgccag cgatgcggcg ctactggcgt cgggtacggc agccctggag
    46861 tgtatgctgg cgaaatgccc gatggtggtg ggatatcgca tgaagccttt taccttctgg
    46921 ttggcgaagc ggctggtgaa aactgattat gtctcgctgc caaatctgct ggcgggtaga
    46981 gagttagtca aagaattatt gcaggaagag tgtgagccgc aaaaactggc tgcggcgctg
    47041 ttaccgctgt tggcgaacgg gaaaaccagc cacgcgatgc acgatacctt ccgtgaactg
    47101 catcagcaga tccgctgcaa tgccgatgag caggcggcac aagccgttct ggagttagca
    47161 caatgatcga atttgtttat ccgcacacgc acctggttgc gggtgtggat gaagtcggac
    47221 gcgggccgtt agttggcgcg gtcgtcaccg ctgcggtgat cctcgacccg gcgcgcccga
    47281 ttgccgggct gaatgattcc aaaaagctga gcgaaaaacg ccgtctggcg ctctgtgaag
    47341 agatcaaaga gaaagcgttg agctggagtc tgggccgcgc ggaaccccac gaaatcgacg
    47401 agctgaacat tcttcatgcg accatgctgg cgatgcagcg tgccgtcgct gggctgcata
    47461 ttgcgccgga atatgtgttg attgatggta accgctgccc gaaattaccg atgcctgcga
    47521 tggctgtggt gaaaggcgat agccgcgtac cggaaatcag tgccgcgtct atcctggcga
    47581 aagtgacgcg tgacgccgaa atggcggcgc tggatattgt tttcccgcaa tatggttttg
    47641 cccaacacaa agggtatcca accgcttttc atctggaaaa actggctgaa tatggcgcga
    47701 ctgaacatca tcgtcgcagc tttgggcctg tcaaacgagc actgggactt gcgtcctgat
    47761 tcttgtcgag attaagtaaa ccggaatctg aagatgtctg aaccacgttt cgtacacctg
    47821 cgggtgcaca gcgactactc gatgatcgat ggcctggcca aaaccgcacc gctggtaaaa
    47881 aaggcggcgg cgttgggtat gccagcactg gcgatcaccg atttcaccaa cctttgcggt
    47941 ctggtgaagt tctacggagc gggacatggc gcagggatta agcctatcgt cggggcagat
    48001 tttaacgtcc agtgcgacct gctgggtgat gagttaaccc acctgacggt actggcggcg
    48061 aacaataccg gctatcagaa tctgacgttg ctgatctcaa aagcgtatca gcgtgggtac
    48121 ggtgccgctg ggccgatcat cgatcgcgac tggcttatcg aattaaatga agggttgatc
    48181 cttctttccg gcgggcgcat gggcgacgtc ggacgcagtc ttttgcgtgg taacagcgcg
    48241 ctggtagatg agtgtgtcgc gttttatgaa gaacacttcc cggatcgcta ttttctcgag
    48301 ctgatccgca ccggcaggcc ggatgaagaa agctatctgc acgcggcggt ggaactggcg
    48361 gaagcgcgcg gtttgcccgt cgtggcgacc aacgacgtgc gctttatcga cagcagcgac
    48421 tttgacgcac acgaaatccg cgtcgcgatc cacgacggct ttaccctcga cgatcctaaa
    48481 cgcccgcgta actattcgcc gcagcaatat atgcgtagcg aagaggagat gtgcgagctg
    48541 tttgccgaca tcccagaagc tcttgccaac accgttgaga tcgccaaacg ctgtaacgtg
    48601 actgtgcgtc ttggtgaata cttcctgccg cagttcccga ccggggacat gagcaccgaa
    48661 gattatctgg tcaagcgtgc aaaagaaggc ctggaagagc gtctggcctt tttattccct
    48721 gatgaggaag aacgtcttaa acgccgcccg gaatatgacg aacgtctgga gactgaactt
    48781 caggttatca accagatggg cttcccgggc tacttcctca tcgttatgga atttatccag
    48841 tggtcgaaag ataacggcgt accggtaggg ccaggccgtg gctccggtgc gggttcactg
    48901 gtggcctacg cgctgaaaat caccgacctc gatccgctgg aatttgacct gctgttcgaa
    48961 cgtttcctta acccggaacg tgtctccatg cctgacttcg acgttgactt ctgtatggag
    49021 aaacgcgacc aggttatcga gcacgtagcg gacatgtacg gtcgtgatgc ggtatcgcag
    49081 atcatcactt tcggtacaat ggcggcgaaa gcggtgatcc gcgacgtagg ccgcgtgctg
    49141 gggcatccgt acggctttgt cgatcgtatc tcgaaactga tcccgcccga tccggggatg
    49201 acgctggcga aagcgtttga agccgagccg cagctgccgg aaatctacga agcggatgaa
    49261 gaagttaagg cgctgatcga catggcgcgc aaactggaag gggtcacccg taacgccggt
    49321 aagcacgccg gtggggtggt tatcgcgccg accaaaatta ccgattttgc gccgctttac
    49381 tgcgatgaag agggcaaaca tccggtcacc cagtttgata aaagcgacgt tgaatacgcc
    49441 ggactggtga agttcgactt ccttggtttg cgtacgctca ccatcatcaa ctgggcgctg
    49501 gagatgatca acaagcggcg ggcgaagaat ggcgagccac cgctggatat cgccgcgatc
    49561 ccgctggatg acaagaaaag cttcgacatg ctgcaacgct cggaaaccac ggcggtattc
    49621 cagcttgaat cgcgcggcat gaaggacctg atcaagcgtc tgcaacctga ctgcttcgaa
    49681 gatatgatcg cactggtggc actgttccgc cctggtccgt tgcagtcagg gatggtggat
    49741 aactttattg accgtaaaca tggtcgcgaa gaaatctcct atccagacgt acagtggcag
    49801 catgaaagcc tgaaaccggt actggagcca acctacggca ttatcctgta tcaggaacag
    49861 gtcatgcaga ttgcccaggt gctttctggt tataccctcg gtggcgcgga tatgctgcgt
    49921 cgagcgatgg gtaagaaaaa gccggaagag atggctaagc agcgttctgt atttgctgaa
    49981 ggtgcagaaa agaacggaat caacgccgaa ctggcgatga aaatcttcga cctggtggag
    50041 aaattcgcgg gttacggatt taacaaatcg cactctgcgg cctatgcttt ggtgtcatat
    50101 caaacgttat ggctgaaagc gcactatccg gcggagttta tggcggcggt aatgaccgcc
    50161 gatatggaca acaccgagaa ggtggtgggc ctggtggatg agtgctggcg gatggggctg
    50221 aaaatcctgc caccagatat aaactccggt ctttaccatt tccacgtcaa cgacgacggc
    50281 gaaatcgtgt atggtattgg cgcgatcaaa ggggtcggtg aaggtccgat tgaggccatc
    50341 atcgaagccc gtaataaagg cggctacttc cgcgaactgt ttgatctctg cgcccgaacc
    50401 gacaccaaaa agttaaaccg gcgggtgctg gaaaaactga tcatgtccgg ggcgtttgac
    50461 cgtcttggac cacaccgcgc ggcactgatg aactcgctgg gcgatgcgtt aaaagcggca
    50521 gatcaacacg cgaaagcgga agctatcggt caggccgata tgttcggcgt gctggccgaa
    50581 gagccggaac aaattgaaca atcctacgcc agctgccaac cgtggccgga gcaggtggta
    50641 ttagatgggg aacgtgaaac gttaggcctg tacctgaccg gacaccctat caaccagtat
    50701 ttaaaagaga ttgagcgtta tgtcggaggc gtaaggctga aagacatgca cccgacagaa
    50761 cgtggtaaag tcatcacggc tgcggggctc gttgttgccg cgcgggttat ggtcaccaag
    50821 cgcggcaatc gtatcggtat ctgcacgctg gatgaccgtt ccgggcggct ggaagtgatg
    50881 ttgtttactg acgccctgga taaataccag caattgctgg aaaaagaccg catacttatc
    50941 gtcagcggac aggtcagctt tgatgacttc agcggtgggc ttaaaatgac cgctcgcgaa
    51001 gtgatggata ttgacgaagc ccgggaaaaa tatgctcgcg ggcttgctat ctcgctgacg
    51061 gacaggcaaa ttgatgacca gcttttaaac cgactccgtc agtctctgga accccaccgc
    51121 tctgggacaa ttccagtaca tctctactat cagagggcgg atgcacgcgc gcggttgcgt
    51181 tttggcgcga cgtggcgtgt ctctccgagc gatcgtttat taaacgatct ccgtggcctc
    51241 attggttcgg agcaggtgga actggagttt gactaataca ggaatactat gagtctgaat
    51301 ttccttgatt ttgaacagcc gattgcagag ctggaagcga aaatcgattc tctgactgcg
    51361 gttagccgtc aggatgagaa actggatatt aacatcgatg aagaagtgca tcgtctgcgt
    51421 gaaaaaagcg tagaactgac acgtaaaatc ttcgccgatc tcggtgcatg gcagattgcg
    51481 caactggcac gccatccaca gcgtccttat accctggatt acgttcgcct ggcatttgat
    51541 gaatttgacg aactggctgg cgaccgcgcg tatgcagacg ataaagctat cgtcggtggt
    51601 atcgcccgtc ttgacggtcg tccggtgatg atcattggtc atcaaaaagg tcgtgaaacc
    51661 aaagaaaaaa ttcgccgtaa ctttggtatg ccagcgccag aaggttaccg caaagcactg
    51721 cgtctgatgc aaatggctga acgctttaag atgcctatca tcacctttat cgacaccccg
    51781 ggggcttatc ctggcgtggg cgcagaagag cgtggtcagt ctgaagccat tgcacgcaac
    51841 ctgcgtgaaa tgtctcgcct cggcgtaccg gtagtttgta cggttatcgg tgaaggtggt
    51901 tctggcggtg cgctggcgat tggcgtgggc gataaagtga atatgctgca atacagcacc
    51961 tattccgtta tctcgccgga aggttgtgcg tccattctgt ggaagagcgc cgacaaagcg
    52021 ccgctggcgg ctgaagcgat gggtatcatc gctccgcgtc tgaaagagct gaaactgatc
    52081 gactccatca tcccggaacc gctgggcggc gctcaccgta acccggaagc gatggcggca
    52141 tcgttgaaag cgcaactgct ggcggatctg gccgatctcg atgtgttaag cactgaagat
    52201 ttaaaaaatc gtcgttatca gcgcctgatg agctacggtt acgcgtaatt cgcaaaagtt
    52261 ctgaaaaagg gtcacttcgg tggccctttt ttatcgccac ggtttgagca ggctatgatt
    52321 aaggaaggat tttccaggag gaacacatga acatcattgc cattatggga ccgcatggcg
    52381 tcttttataa agatgagccc atcaaagaac tggagtcggc gctggtggcg caaggctttc
    52441 agattatctg gccacaaaac agcgttgatt tgctgaaatt tatcgaacat aaccctcgaa
    52501 tttgcggcgt gatttttgac tgggatgagt acagtctcga tttatgcagc gatatcaatc
    52561 aacttaatga atatctcccg ctttatgcct tcatcaacac ccactcgacg atggatgtca
    52621 gcgtgcagga tatgcggatg gcgctctggt tttttgaata tgcgctgggg caggcggaag
    52681 atatcgccat tcgtatgcgt cagtacaccg acgaatatct tgataacatt acaccgccgt
    52741 tcacgaaagc cttgtttacc tacgttaaag agcggaagta caccttttgt acgccggggc
    52801 atatgggcgg caccgcatat caaaaaagcc cggttggctg cctgttttat gattttttcg
    52861 gcgggaatac cctcaaggct gatgtctcta tttcggtcac cgagcttggt tcgttgctcg
    52921 accacaccgg gccacacctg gaagcggaag agtacatcgc gcggactttt ggcgcggaac
    52981 agagttatat cgttaccaac ggaacatcga cgtcgaacaa aattgtgggt atgtacgccg
    53041 cgccatccgg cagtacgctg ttgatcgacc gcaattgtca taaatcgctg gcgcatctgt
    53101 tgatgatgaa cgatgtagtg ccagtctggc tgaaaccgac gcgtaatgcg ttggggattc
    53161 ttggtgggat cccgcgccgt gaatttactc gcgacagcat cgaagagaaa gtcgctgcca
    53221 ccacgcaggc acaatggccg gttcatgcgg tgattaccaa ctccacctat gatggcttgc
    53281 tctacaacac cgactggatc aaacagacgc tggatgtccc gtcgatccac ttcgattccg
    53341 cctgggtgcc gtacacccat tttcatccga tctaccaggg gaaaagtggt atgagcggcg
    53401 agcgtgttgc gggaaaagtg atcttcgaaa cgcaatcgac ccacaaaatg ctggcggcgt
    53461 tatcgcaggc ttcgctgatc cacattaaag gcgagtatga cgaagaggcg tttaacgaag
    53521 cctttatgat gcataccacc acctcgccca gttatcccat tgttgcttcg gttgagacgg
    53581 cggcggcgat gctgcgtggt aatccgggca aacggctgat taaccgttcg gtggagcggg
    53641 cgctgcattt tcgcaaagag gtccagcggt tgcgggaaga gtctgacggt tggtttttcg
    53701 atatctggca accgccgcag gtggatgaag ccgaatgctg gcccgttgcg ccaggcgaac
    53761 agtggcacgg ctttaacgat gcggatgccg atcatatgtt tctcgatccg gttaaagtca
    53821 ctattttgac accggggatg gacgaacagg gcaatatgag cgaggagggg atcccggcag
    53881 cgctggtggc aaaattcctc gacgaacgtg ggatcgtagt agagaaaacc ggcccttata
    53941 acctgctgtt tctctttagt attggtatcg ataaaaccaa agcaatggga ttattgcgtg
    54001 ggttgacgga atttaaacgc tcttacgatc tcaacctgcg gatcaaaaat atgctacccg
    54061 atctctatgc tgaagatccc gatttctacc gcaatatgcg tattcaggat ctggcgcaag
    54121 ggatccataa gctgattcgt aaacacgatc ttcccggttt gatgttgcgg gcattcgata
    54181 ctttgccgga aatgatcatg acgccacatc aggcatggca gagacagatt aaaggtgaag
    54241 tagaaaccat tgcgctggaa caactggtcg gtagagtatc ggcaaatatg atcctgcctt
    54301 atccaccggg cgtaccgctg ttgatgccag gagaaatgct gaccaaagag agccgcacgg
    54361 tactcgattt tctactgatg ctttgttccg tagggcaaca ttaccccggt tttgaaacgg
    54421 atattcacgg cgcgaaacag gacgaagacg gcgtttaccg cgtacgagtc ctaaaaatgg
    54481 cgggataact tgccagagcg gcttccgggc gagtaacgtg ctgttaacaa ataaaggaga
    54541 cgttatgctg ggtttaaaac aggttcacca tattgcgatt attgcgacgg attatgcggt
    54601 gagcaaagct ttctactgcg atattcttgg tttcacgctg caaagcgaag tctatcgcga
    54661 agcgcgcgac tcgtggaaag gggatttggc gcttaatggg caatatgtga ttgagctttt
    54721 ctcttttccg ttcccgccgg agcgccccag ccgaccggaa gcttgcggcc tgcgtcatct
    54781 ggcttttagc gttgatgata tcgatgcggc agtggcgcac cttgaaagcc ataacgtgaa
    54841 gtgtgaagcc atccgtgtcg atccatacac gcaaaaacgc ttcacgttct ttaacgatcc
    54901 ggacgggctg ccgttggaac tgtatgagca gtaaggcttg tcatcgccgc atttgcccgg
    54961 taacgtgccg ggcattgcta ctgtaaaatc gcaccatcat gacactcacg ctcaatagac
    55021 aacttctcac cttacgccag attctggtgg cctttagcgg cgggcttgac tccaccgttc
    55081 tgctgcatca gttggtgcag tggcggacgg aaaatccggg cgtcactcta cgcgctatcc
    55141 atgtgcatca cggtttaagt gccaatgccg atgcctgggt taggcattgc gagaacatct
    55201 gccaacagtg gcaggtgccg ctggtggtcg agcgcgtgca acttgcgcaa gaaggactgg
    55261 gcattgaggc ccaggcgcgg caggcgcgtt atcaggcatt tgcccgcacc ttgttgcccg
    55321 gtgaagtgct ggtcaccgcg caacatctcg acgatcaatg tgaaactttt ctgctggcgc
    55381 taaaacgcgg tagcggccct gccgggcttt cggctatggc ggaagtttcg gagtttgccg
    55441 gaacacggct tatccgcccg ttgcttgccc gcacgcgggg ggaactggcg cagtgggcat
    55501 tggcgcatgg tttacgctgg attgaagacg aaagtaatca ggacgactca tacgatcgta
    55561 actttctgcg cctgcgcgta gtgccgttat tgcagcagcg ttggccgcat tttgccgaag
    55621 caacggcccg cagcgctgcg ctttgcgctg aacaagagag cctgttagat gaactgctgg
    55681 cggatgatat agcgcactgt caaaagccgc aggggacgct gcagattgcg ccaatgctgg
    55741 cgatgagtga tgcccgtcgc gcggcgatta tccgccgctg gctggcaggg aagaatgcac
    55801 cgatgccttc ccgcgacgcg ttggtgcgga tctggcagga agtggcgctg gcgcgggaag
    55861 atgcctcccc ctgtttacgc ttgggcgcgt ttgaaatccg ccgctatcag tcgcaactgt
    55921 ggtggattaa atccgtcacc gggcaaagtg aaaccattgt gccgtggcag acgtggctac
    55981 aaccgctgga attaccggcg gggctgggaa gcgtacagct tactgcggga ggcgatattc
    56041 gccctccgcg tgcagacgaa gcggtcagcg tgcgtttcaa agcgccagga ttgctgcata
    56101 ttgtcggtcg caacggcgga cgtaagctaa agaaaatctg gcaagagctg ggcgtgccgc
    56161 cgtggctacg tgacaccacg ccactgctgt tttatggcga aacgctgatt gcggcggcag
    56221 gggtatttgt gacgcaagaa ggtgtggctg aaggtgagaa gggcgtaagt tttgtctggc
    56281 agaaaacgct tagttaagtg aaagccggat aagacgcatc aaacgtcgca tccggcgaaa
    56341 gtcaatcagg attcgcttac caccaccgta ccgatttccg ggtggctaaa gctggtaatt
    56401 ttatccagac gcagctcgcg ggtttcgcca gcggcctcga cgactagata ctccacattt
    56461 ttgcgggaga ctaaatcact ggcttttgcc tgcaattttt cgccatcttt cagctcaagt
    56521 gtcagcatta aatgatgctg gcaggcgagc tcaagattat cgtaatcatc acaattgatt
    56581 ggttgatacg tatcattcat tgacataatc gctcaccagt aagtttgccg cagcgtatgc
    56641 tgctttttcc ctgacagcct cagaaagggc gtcgtcggca gccatttcat tcagcacttt
    56701 caaaacgcag cccagcgcgt ccggaacgta tcctaagtct ccgctggcga tttccgcgta
    56761 ccgcttgcgt attaactcac aatatttttc cacatgccct cctgtcagca ctctgactta
    56821 accgtggatg caagtctaag cctacgaaga taaactctgt ttcgcaaggt gactatacca
    56881 cactcatttc tgcaatatca gcgccgcaac tgcacgtatt ccgttacaat ggcctcctga
    56941 ttcgaaagga gttttcttat ggcgcttaaa gcgacaattt ataaagcgac ggttaatgtg
    57001 gccgatctcg accgtaacca gtttctcgat gcctctctga cgctggcgcg ccacccttca
    57061 gaaacccagg agcgtatgat gctgcgcttg ctggcgtggt tgaaatatgc tgatgaacgt
    57121 ctgcaattta cccgtggttt gtgtgccgat gatgagccgg aagcgtggct gcgtaacgat
    57181 cacctgggca ttgatttgtg gattgagctg gggctgccgg atgagcggcg gattaagaaa
    57241 gcctgcaccc aggccgcaaa agtggcgctg tttacctata atagccgggc ggcgcaaatc
    57301 tggtggcagc aaaatcagag caaatttgtg cagtttgcca atctttccgt ctggtatctg
    57361 gacgatgaac aactggcgaa agtaagcgcc tttgccgatc gtaccatgac gctgcaggca
    57421 acgattcagg atggcgtgat ctggttatcg gatgataaga ataatctgga agtgaactta
    57481 accgcctggc aacaaccttc atgattgtga tttcccgaca tgttgctatt cccgatggtg
    57541 agcttgagat caccgccatt cgtgcgcagg gcgcgggcgg gcagcatgtt aataagacct
    57601 caacggctat tcatctgcgt tttgacattc gggcgtccag cctgccagag tattacaaag
    57661 agcgtctgct cgccgccagc catcatttga tcagcagtga tggcgtgatt gtcattaagg
    57721 cacaggaata ccgcagtcag gaactgaacc gcgaagcggc gctggcccgg ctggtggcag
    57781 tgattaaaga tttaacaaca gaacaaaaag cccgacgacc cacgcggccc acccgtgcat
    57841 cgaaagagcg caggctggca tcgaaagcac aaaaatcaag cgtgaaggcg atgcgcggca
    57901 aagtacgcag cggtcgggaa taaaaagaag gaatggatgg tgaaaaaagc gatagtgaca
    57961 gcgatggctg taatcagcct ctttactctg atgggatgta ataatcgggc cgaagtcgat
    58021 acgctttctc cggcgcaggc tgccgaacta aaaccgatgc cgcaaagttg gcgcggcgtg
    58081 ctgccgtgtg ccgattgcga agggatcgaa acctctctgt tcctcgaaaa agacggaaca
    58141 tgggtgatga atgagcgtta tctcggtgct cgtgaagaac cttcctcctt cgcttcctac
    58201 ggtacatggg cgcgaaccgc tgacaagctg gtattaaccg acagcaaagg tgaaaagtca
    58261 tattatcggg cgaaaggcga tgcgctggag atgctcgatc gtgaaggcaa tccaattgaa
    58321 tcgcagttca actatacgct ggaaccggca caatccagtt tacctatgac gccgatgacc
    58381 ctgcgaggca tgtattttta tatggctgat gcggcgacct tcactgattg cgcgaccgga
    58441 aaacgtttca tggtagcgaa taacgcagag ctggagcgta gctacctggc tgcgcgcggt
    58501 cacagtgaaa aaccggtgtt actgtcagta gaaggtcact ttacgcttga ggctaatccg
    58561 gatactggtg cgccaaccaa agtattagcg cccgatacgg cgggtaaatt ttacccgaat
    58621 aaggattgca gtagtttggg gcagtaaccc gtcttgagac agaaacaaac gcaaaattgc
    58681 ctgatgcgct gcgcttatca ggcctacagc atataatgca acattttgaa ttagcacgat
    58741 tttgtaggcc ggataaggcg tttacgccgc atccggcatt ttgtgcacga ggctggcttt
    58801 atcactgagt cagcccaaca aagcgacccg ccttgatata aatcccaggc ttcaggtgcc
    58861 caacatactg taactgcgtt tctggcttaa atgcagagac atcgccagta cgcatatgca
    58921 tcagatcggc agggctaatc cagcccgatt gcgccagcgt aagcggatgc ccggctttgg
    58981 caaatgcatc agtgacaaac tccgaacaaa accacgactt tttgtctcct tcgcccacac
    59041 tgcttaactg cgctttcgcc aggccgctga cgcactgttg gcgaaaatct gcggaaaaag
    59101 gattcagtga gcacatctgg cgggtcacca taaatggaat gaactcgaca atgccgcgat
    59161 agttataccc gctatctttg attttgttgg cgaacgcggt gatttctgtg gcttgttgcg
    59221 gggtaagatc cgggactcgt aagacgaaaa gcttatcgct atgcttcatc gcttttttaa
    59281 gggaaacaat ctggacgcca gcgcctgtcg cttctgcaac gttattttca cccagataga
    59341 ttgcaacgtg actcacagag gaagtgctga agacgcggat tccaaatgag gttaccccaa
    59401 ggcttgagga gaacagcaaa tcgccgggtt tgagatcagg tgccgttatt tcttttattg
    59461 attgttcggt gaaagagctt tgatgctgga atttgacagc ccatgttttt gcctcggcat
    59521 ctactgctgt ggctgatggg tctggctggc tgatatcaac ggtacaagcc gaaagtaaaa
    59581 gaaagctggg gagaagcagg cggcagtacg cctttggttt atccatttta tacaatccat
    59641 gtaaaaaagg gccctgaaat tcaggaccct ttctggcatc agcctttaat ctgtttcacc
    59701 agatattcga cgatgtcacc agtcttaatt aactgtttct cgccgttgcg acgatattta
    59761 tattcgatat cgtcgttgtc gaggttacgg tcgcccagca caatagtgtg cggaataccg
    59821 atcagttcca tatcagcaaa catcacgccc ggacgctctt tgcggtcatc cagcagcact
    59881 tcgatacctt gtgcacgcag ttcgctgtac agtttctcag caagctcttg tacgcggaag
    59941 gatttgtgca tgttcatcgg cagaatcgcc acctggaacg gcgcgatcgc gtcaggccat
    60001 acgatgccgc gttcgtcgta gttctgctcg atcgccgcag ctactacacg cgttaccccg
    60061 ataccgtagc aacccatcgt caggatttga ttacggccat cttcaccctg tacggaggct
    60121 ttcagtgctt cggagtactt ggtacccagc tggaagatgt gaccaacttc gataccacgt
    60181 ttgatcagca gcgtaccctg accatccggg cttggatcgc ccgccaccac gttgcggata
    60241 tcagcaattt ccggggtagc gacatcgcga tcccagttga taccgaagta gtgtttacca
    60301 tcgatgttag caccagcagc gaaatcactc atcgccgcaa cggtacggtc aatcaccacc
    60361 ggaatcggca tgtttaccgg acccagtgaa cccggaccgg ctttaaccac ggcacgaatt
    60421 tcttcttcag tcgcaaaagt cagcgggctg gcaacctgcg gcagtttttc tgctttaact
    60481 tcgttcagct cgtggtcacc gcgcaccagc agcgcaacca acgggaagct actgccttca
    60541 accgctttaa ccagcagagt cttaaccgtt ttctcaatcg gcagattgaa ctgttcaacc
    60601 agttccgcga tggttttcgc gttcggcgta tcaaccagcg tcatttcctg ggtagccgca
    60661 gcgcgcggtt ctttcggcgc gatagcttct gccagttcaa tgttcgctgc atagtcagag
    60721 gtgtcggaga agaccacatc gtcttcaccg ctctgcgcca gcacctggaa ttcgtgagag
    60781 gcgctgccgc cgatagaacc ggtgtcggcc tgtacggcgc ggaaatccag ccccatgcgg
    60841 ctgaagattt tgctgtaggc cgcatacatt gcatcgtagg tttcctgcag ggattcctga
    60901 gaagtatgga aagagtaagc atctttcatc aggaattcgc gggaacgcat gacgccgaaa
    60961 cgcggacgca cttcgtcgcg gaacttggtc tggatctgat agaagttcag cggcagctgt
    61021 ttgtaagagc taagctcgtt acgaatcagg tcagtgataa cttcttcatg agttgggccg
    61081 agtacgaacg gacgctcgcc acggtcaaca aaacgcagca gttccggacc gtactgttcc
    61141 caacgaccac tctcttgcca caaatcggct ggctgaacca ccggcatcga cacctcgatt
    61201 gcaccggcgt tgttcatctc ttcacgcacg atgttttcga cttttttcag aacgcgcacg
    61261 ccggtcggca gccaggtata taacccggag gccagcttgc ggatcatccc ggcgcgcagc
    61321 atcagctgat ggctgatcac ctcggcgtcg gcaggtgtct ccttgagagt ggagagcagg
    61381 tattggctag tacgcatgtt gttacggttc cagttggaag gtagaacagg ctcaaggcga
    61441 gcctgggaca aaaaaagtga tttagtttac cagtgcaaaa gaaatgtcaa aagagaaggg
    61501 cgtgaattta aggcggttcc agcgcaaaga cttcaaaacc tgcgtcggtg acgcgccagc
    61561 gaacgttaaa atcatgtagc cagacggcat aggttttgcc cgtttcctca cctttacgat
    61621 aggccgggcg cgggtcctgc gccagtactt cgcggataaa cagcgttaac tgcggataac
    61681 gcttctccag cgtcaaaagc tgcttttcga cctctgcggt aaaactcacc gccatttctg
    61741 ccgctggcgc actttgcgca tagctggcac tggcatcggg aagcgattcg gcaaagggga
    61801 gatacggttt gatatccact accggcgtac catcgaccag atccagactg ccgagcttca
    61861 gaatcacgct gtctttatgg caaacaacct ctttcagctc taccagcgac ataccaattg
    61921 ggttagggcg gaaagtagag cgcgtagcga aaacacccat tctggcatta ccgccaagac
    61981 gaggcggacg cacagtcgga cgccagccgc cttccatcgt ttgatgaaag acgaaaagta
    62041 tccataaatg gctgaacgct tccaggccgc gaacggcgtc ggcctggttg tagggagcca
    62101 tgagatgcag ttcgccgttg gcgcttttta ccagacctgg ctggcgcgga acggcgaatt
    62161 tttctttata gggcgagcga ataacgccta tttgctcgaa ctggaaactg ctcatttcgc
    62221 cgtaatgtta agcgcagagc cgatacatac agcctgacga tagcagcctg gcgtaccgct
    62281 ggtaacttcg cagctatgca gtaataccgc attggctttc attttcgagg cgttgatttg
    62341 catccgctta cgtgcggttg gaatgctcgg cggagagtcc tgattagagg cctggcaaga
    62401 gtcgccactg acttcaccga gatcgcggaa cggtttgccg actaattctt ctgcattggt
    62461 ataaattcgg accggcgtgg cgcgcggcgc tttcggtttt gcaggctccg ctttcggctg
    62521 gggtgcagtg ctttgaacgg gttcgacagg ggatctgctt aacatggaac agccgcttag
    62581 catgagtgct actaaacaga tcggtaaagc acgcatagta tttcctcaat gtatgatcaa
    62641 aacgtcaata ttgaatcagg agcttgtaaa aatgacaaga cgggcaagcg cccgtcctga
    62701 atgatattac aaattgtgga aacagcctaa aaattaccag cctttaacag cgccgccgtt
    62761 aaacactttg tttgctgctt cgtaaacttc atcagactga taagcctgga cgaatttctt
    62821 cacgttctcg gcgtctttgt tatcttcacg cgttacgatc aggtttacgt acggggagtc
    62881 tttatcttcg acaaagatac cgtctttcgc tggagtcagg ccaatctggc tggcataggt
    62941 ggtattgata actgccagag cgatttgcgc gtcgtccaga gatcgaggca gctgcggtgc
    63001 ttccagctca acaattttca gattttttgg gttctcaaca acatcaagaa cggtcggcag
    63061 caggccaacg ccatctttca gtttgatcaa gcccactttt tgcagcagca gcagtgaacg
    63121 accaagatta gttggatcgt ttggcacggc aacctgcgaa ccatcctgca gttcatccag
    63181 tgatttgatt ttcttggagt aaccagcaat cggataaaca aaagtgttgc ctactgcgac
    63241 cagtttgtag ccacgatctt tcagttgctg atcaaggtac ggtttatgct ggaaggcgtt
    63301 ggcgtcgata tcgcctttgc tcaatgcttc gtttggcaga acatagtcgt tgaaggttac
    63361 cagctcaacg tccaggccat atttgtcttt cgcaactttc tgcgcgactt ctgctacctg
    63421 ctgttcggca ccaacaatca cgccgacttt aatgtggttt ggatcttttt catcctgacc
    63481 gcagcctacc agtgccagtg atccaatcag ggctcccact gccgcaaagg ttttgaattt
    63541 gaacgccata ccttattcct tcttcaatta tttatgttgt gttgaacgtt acttgcgagt
    63601 gacagcccgg acgatgcggt cgcctgcgaa ctgaattaaa taaaccagaa tgaccagcaa
    63661 taccagtacc gtattcatca ccgtcgcgtt atagccgatg tagccatact gatagccaat
    63721 ctgacctaaa ccaccggcac cgactgcacc acccatcgcg gaataaccga ccagggtaat
    63781 cagggtgata gttgccgcat tcaccagacc cggcagcgct tccggtaaca gcaccttacg
    63841 gacgatctgc atcggcgtgg cacccattgc gcgggaagct tcaattaacc cggttgggat
    63901 ctctagcaga gcgttctcga ccatacgggc aataaacggc gctgcgccaa cggttaacgg
    63961 aacaatcgcc gcctgtaaac caatcgatgt accgacaata actcgggtaa acggaatcat
    64021 ccataccagc aagataatga acgggatgga acggaaaatg ttcacaattg cagaaacggt
    64081 acgatacagc ttcgcgttag caataatttg ccccggacgc gtgacataaa gcagaacgcc
    64141 aaccggcaga ccaatcacaa agccaaaaaa accggatacg aaggtcattg ccagcgtttc
    64201 ccatacgcca cgaaccagca gccacatcat cggctcagac ataacccagt acctctactt
    64261 ttacatggtg ttcctgcagc caggcaatgg cggcttgcgt atcttgttgt gtgccgtgca
    64321 tttcggtcag catgatgccg aacttaacgc caccggcgta atccatctgc gcgctaataa
    64381 tgttgttgtt gacgttgaaa cgacgcgcgg tttcagaaag cagtggggca tcgaccgatt
    64441 gaccggtaaa ctccagacgc agcatcggca cgcagtcagt aaatggctcc gcttgcagac
    64501 gttcctggta atcttccggg atatccagat gcagggtcga ctgaataaac ttctgcgcca
    64561 gcggcgtttt cggatgcgag aacacttcac ttaccgtgtc ctgctcgatc agttcgccat
    64621 tgctgatgac cgccacgcaa tcacaaatgc gcttcacaac gtccatttcg tgagtgatca
    64681 acagaatcgt caaacccaga cggcggttga tgtctttcag cagttcgaga atagaacgtg
    64741 tcgttgccgg gtccagcgcg ctggtggctt catcacacag caatactttg ggattgctgg
    64801 ctaatgcacg ggcaatcgcc acacgttgtt tctgcccacc ggaaagattc gacgggtagc
    64861 tatcatgctt atcgccaaga ccaaccaatg acagcaattc cgtcacgcga cgtttgatct
    64921 cgtctttcgg tgtgttgtcc agctccagcg gcagagccac gttgccaaaa acagtacgcg
    64981 aagagagcag gttaaaatgc tggaaaatca taccaatctg gcggcgagct ttggtcaact
    65041 cggattctga cagcgtggtc agttcctggc catcgaccag cacgctaccc tcggttgggc
    65101 gctccagcag gtttacacaa cgtataagcg tactcttacc cgcgcctgag gcaccgataa
    65161 cgccataaat ttgtccagct ggcacatgca ggctgacgtt gttcaacgcc tggatggtgc
    65221 gggtgccctg gtggaacact ttggtgatat tcgaaagttt tatcattgat tatttattat
    65281 cgtcattaag ttagtcgtgg catctcgaat gcctgaaacg ggcaacgccg tcaatgaaat
    65341 ggatgttaag gcatccagac gtctaaatca atcaggttta tgcgaagagc actttcttgc
    65401 aggtcgaaac atgcgatact agcgtcacat gccttattaa ggagctataa aaggtggcga
    65461 agagcgtacc cgcaattttt cttgaccgtg atggcaccat taatgtcgat cacggctatg
    65521 tccatgagat cgacaacttt gaatttatcg acggtgttat tgacgccatg cgcgagctaa
    65581 aaaaaatggg ctttgcgctg gtggtagtaa ccaaccagtc tggcattgct cgcggtaaat
    65641 ttaccgaagc acagtttgaa acgctgaccg agtggatgga ctggtcgctg gcggaccgag
    65701 atgtcgatct ggatggtatc tattattgcc cgcatcatcc gcagggtagt gttgaagagt
    65761 ttcgccaggt ctgcgattgc cgcaaaccac atccggggat gcttttgtca gcacgcgatt
    65821 atttgcatat tgatatggcc gcttcttata tggtgggcga taaattagaa gatatgcagg
    65881 cagcggttgc ggcgaacgtg ggaacaaaag tgctggtgcg tacgggtaaa cctattacac
    65941 ctgaagcaga aaacgcggca gattgggtgt taaatagcct ggcagacctg ccgcaagcga
    66001 taaaaaagca gcaaaaaccg gcataatgat taaaagatga gcgattgaaa taaaaatgca
    66061 tttttccact tgtcttcctg agacgactcc ctataatgcg cctccatcga cacggcggat
    66121 gtgaatcact tcacacaaac agccggttcg gttgaagaga aaaatcctga aattcagggt
    66181 tgactctgaa agaggaaagc gtaatatacg ccacctcgcg acagtacgct aaagcgtgtc
    66241 gcaactgctc tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct
    66301 taacgtcgca agacgaaaaa tgaataccaa gtctcaagag tgaacacgta attcattacg
    66361 aagtttaatt ctttgagcat caaactttta aattgaagag tttgatcatg gctcagattg
    66421 aacgctggcg gcaggcctaa cacatgcaag tcgaacggta acaggaag
//
LOCUS       DAJOWB010000013        63714 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJOWB010000013 DAJOWB010000000
VERSION     DAJOWB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.013"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            143..478
                     /gene="eutS"
                     /locus_tag="OHO85_000941"
     CDS             143..478
                     /gene="eutS"
                     /locus_tag="OHO85_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCQ2670361.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            491..970
                     /gene="eutP"
                     /locus_tag="OHO85_000942"
     CDS             491..970
                     /gene="eutP"
                     /locus_tag="OHO85_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCQ2670362.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            945..1646
                     /gene="eutQ"
                     /locus_tag="OHO85_000943"
     CDS             945..1646
                     /gene="eutQ"
                     /locus_tag="OHO85_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCQ2670363.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1643..2446
                     /gene="eutT"
                     /locus_tag="OHO85_000944"
     CDS             1643..2446
                     /gene="eutT"
                     /locus_tag="OHO85_000944"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCQ2670364.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            2443..3459
                     /gene="pta"
                     /locus_tag="OHO85_000945"
     CDS             2443..3459
                     /gene="pta"
                     /locus_tag="OHO85_000945"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCQ2670365.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTKVNRESSLQ
                     TLVE"
     gene            3498..3791
                     /gene="eutM"
                     /locus_tag="OHO85_000946"
     CDS             3498..3791
                     /gene="eutM"
                     /locus_tag="OHO85_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCQ2670366.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3898..4185
                     /gene="eutN"
                     /locus_tag="OHO85_000947"
     CDS             3898..4185
                     /gene="eutN"
                     /locus_tag="OHO85_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCQ2670367.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4197..5600
                     /locus_tag="OHO85_000948"
     CDS             4197..5600
                     /locus_tag="OHO85_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCQ2670368.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            5611..6447
                     /gene="eutJ"
                     /locus_tag="OHO85_000949"
     CDS             5611..6447
                     /gene="eutJ"
                     /locus_tag="OHO85_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCQ2670369.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            6437..7624
                     /gene="eutG"
                     /locus_tag="OHO85_000950"
     CDS             6437..7624
                     /gene="eutG"
                     /locus_tag="OHO85_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HCQ2670370.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGLGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWLCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            7741..8967
                     /gene="eutH"
                     /locus_tag="OHO85_000951"
     CDS             7741..8967
                     /gene="eutH"
                     /locus_tag="OHO85_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HCQ2670371.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            8964..10367
                     /gene="eutA"
                     /locus_tag="OHO85_000952"
     CDS             8964..10367
                     /gene="eutA"
                     /locus_tag="OHO85_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCQ2670372.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            10379..11740
                     /gene="eutB"
                     /locus_tag="OHO85_000953"
     CDS             10379..11740
                     /gene="eutB"
                     /locus_tag="OHO85_000953"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HCQ2670373.1"
                     /translation="MKLKTTLFSNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            11761..12648
                     /gene="eutC"
                     /locus_tag="OHO85_000954"
     CDS             11761..12648
                     /gene="eutC"
                     /locus_tag="OHO85_000954"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HCQ2670374.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            12658..13317
                     /gene="eutL"
                     /locus_tag="OHO85_000955"
     CDS             12658..13317
                     /gene="eutL"
                     /locus_tag="OHO85_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCQ2670375.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            13330..13830
                     /gene="eutK"
                     /locus_tag="OHO85_000956"
     CDS             13330..13830
                     /gene="eutK"
                     /locus_tag="OHO85_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCQ2670376.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            13876..14928
                     /gene="eutR"
                     /locus_tag="OHO85_000957"
     CDS             13876..14928
                     /gene="eutR"
                     /locus_tag="OHO85_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCQ2670377.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(14934..15833)
                     /gene="hemF"
                     /locus_tag="OHO85_000958"
     CDS             complement(14934..15833)
                     /gene="hemF"
                     /locus_tag="OHO85_000958"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCQ2670378.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(15837..16706)
                     /gene="amiA"
                     /locus_tag="OHO85_000959"
     CDS             complement(15837..16706)
                     /gene="amiA"
                     /locus_tag="OHO85_000959"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCQ2670379.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            16920..17345
                     /locus_tag="OHO85_000960"
     CDS             16920..17345
                     /locus_tag="OHO85_000960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCQ2670380.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            17332..17781
                     /locus_tag="OHO85_000961"
     CDS             17332..17781
                     /locus_tag="OHO85_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCQ2670381.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            17842..18417
                     /locus_tag="OHO85_000962"
     CDS             17842..18417
                     /locus_tag="OHO85_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCQ2670382.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            18513..19412
                     /gene="yfeX"
                     /locus_tag="OHO85_000963"
     CDS             18513..19412
                     /gene="yfeX"
                     /locus_tag="OHO85_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCQ2670383.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(19470..20774)
                     /gene="pbp4b"
                     /locus_tag="OHO85_000964"
     CDS             complement(19470..20774)
                     /gene="pbp4b"
                     /locus_tag="OHO85_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HCQ2670384.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(20779..22203)
                     /gene="murP"
                     /locus_tag="OHO85_000965"
     CDS             complement(20779..22203)
                     /gene="murP"
                     /locus_tag="OHO85_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCQ2670385.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22207..23103)
                     /gene="murQ"
                     /locus_tag="OHO85_000966"
     CDS             complement(22207..23103)
                     /gene="murQ"
                     /locus_tag="OHO85_000966"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCQ2670386.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            23267..24124
                     /gene="murR"
                     /locus_tag="OHO85_000967"
     CDS             23267..24124
                     /gene="murR"
                     /locus_tag="OHO85_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCQ2670387.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     DISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            24253..25044
                     /gene="ucpA"
                     /locus_tag="OHO85_000968"
     CDS             24253..25044
                     /gene="ucpA"
                     /locus_tag="OHO85_000968"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCQ2670388.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            25215..26231
                     /gene="cysP"
                     /locus_tag="OHO85_000969"
     CDS             25215..26231
                     /gene="cysP"
                     /locus_tag="OHO85_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCQ2670389.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            26231..27064
                     /gene="cysT"
                     /locus_tag="OHO85_000970"
     CDS             26231..27064
                     /gene="cysT"
                     /locus_tag="OHO85_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCQ2670390.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLILLFSINTLQSRFGRRVVG
                     H"
     gene            27064..27939
                     /gene="cysW"
                     /locus_tag="OHO85_000971"
     CDS             27064..27939
                     /gene="cysW"
                     /locus_tag="OHO85_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCQ2670391.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            27929..29026
                     /gene="cysA"
                     /locus_tag="OHO85_000972"
     CDS             27929..29026
                     /gene="cysA"
                     /locus_tag="OHO85_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCQ2670392.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            29160..30071
                     /gene="cysM"
                     /locus_tag="OHO85_000973"
     CDS             29160..30071
                     /gene="cysM"
                     /locus_tag="OHO85_000973"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCQ2670393.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(30074..30442)
                     /locus_tag="OHO85_000974"
     CDS             complement(30074..30442)
                     /locus_tag="OHO85_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCQ2670394.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            30547..31398
                     /gene="pdxK"
                     /locus_tag="OHO85_000975"
                     /gene_synonym="thiJ"
     CDS             30547..31398
                     /gene="pdxK"
                     /locus_tag="OHO85_000975"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCQ2670395.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(31440..31949)
                     /gene="crr"
                     /locus_tag="OHO85_000976"
     CDS             complement(31440..31949)
                     /gene="crr"
                     /locus_tag="OHO85_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCQ2670396.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(31990..33717)
                     /gene="ptsI"
                     /locus_tag="OHO85_000977"
     CDS             complement(31990..33717)
                     /gene="ptsI"
                     /locus_tag="OHO85_000977"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCQ2670397.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(33762..34019)
                     /gene="ptsH"
                     /locus_tag="OHO85_000978"
     CDS             complement(33762..34019)
                     /gene="ptsH"
                     /locus_tag="OHO85_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCQ2670398.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(34403..35374)
                     /gene="cysK"
                     /locus_tag="OHO85_000979"
     CDS             complement(34403..35374)
                     /gene="cysK"
                     /locus_tag="OHO85_000979"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCQ2670399.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(35559..36320)
                     /gene="cysZ"
                     /locus_tag="OHO85_000980"
     CDS             complement(35559..36320)
                     /gene="cysZ"
                     /locus_tag="OHO85_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCQ2670400.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            36550..37536
                     /gene="zipA"
                     /locus_tag="OHO85_000981"
     CDS             36550..37536
                     /gene="zipA"
                     /locus_tag="OHO85_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCQ2670401.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            37607..39622
                     /gene="ligA"
                     /locus_tag="OHO85_000982"
     CDS             37607..39622
                     /gene="ligA"
                     /locus_tag="OHO85_000982"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCQ2670402.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            39624..39842
                     /locus_tag="OHO85_000983"
     CDS             39624..39842
                     /locus_tag="OHO85_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCQ2670403.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(39839..40837)
                     /locus_tag="OHO85_000984"
     CDS             complement(39839..40837)
                     /locus_tag="OHO85_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCQ2670404.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            40927..41853
                     /locus_tag="OHO85_000985"
     CDS             40927..41853
                     /locus_tag="OHO85_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2670405.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(41844..42185)
                     /locus_tag="OHO85_000986"
     CDS             complement(41844..42185)
                     /locus_tag="OHO85_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCQ2670406.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQCLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(42306..42381)
                     /locus_tag="OHO85_000987"
     tRNA            complement(42306..42381)
                     /locus_tag="OHO85_000987"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42346..42348),aa:Lys,seq:ttt)
     gene            complement(42386..42461)
                     /locus_tag="OHO85_000988"
     tRNA            complement(42386..42461)
                     /locus_tag="OHO85_000988"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42426..42428),aa:Val,seq:tac)
     gene            complement(42508..42583)
                     /locus_tag="OHO85_000989"
     tRNA            complement(42508..42583)
                     /locus_tag="OHO85_000989"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42548..42550),aa:Val,seq:tac)
     gene            complement(42628..42703)
                     /locus_tag="OHO85_000990"
     tRNA            complement(42628..42703)
                     /locus_tag="OHO85_000990"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42668..42670),aa:Val,seq:tac)
     gene            42962..44377
                     /gene="gltX"
                     /locus_tag="OHO85_000991"
     CDS             42962..44377
                     /gene="gltX"
                     /locus_tag="OHO85_000991"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCQ2670407.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(44429..44821)
                     /locus_tag="OHO85_000992"
     CDS             complement(44429..44821)
                     /locus_tag="OHO85_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCQ2670408.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(44823..45182)
                     /locus_tag="OHO85_000993"
     CDS             complement(44823..45182)
                     /locus_tag="OHO85_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCQ2670409.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGIQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            45403..45478
                     /locus_tag="OHO85_000994"
     tRNA            45403..45478
                     /locus_tag="OHO85_000994"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45436..45438,aa:Ala,seq:ggc)
     gene            45518..45593
                     /locus_tag="OHO85_000995"
     tRNA            45518..45593
                     /locus_tag="OHO85_000995"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45551..45553,aa:Ala,seq:ggc)
     gene            45801..47990
                     /locus_tag="OHO85_000996"
     CDS             45801..47990
                     /locus_tag="OHO85_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HCQ2670410.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMINILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(48040..49242)
                     /gene="nupC"
                     /locus_tag="OHO85_000997"
     CDS             complement(48040..49242)
                     /gene="nupC"
                     /locus_tag="OHO85_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCQ2670411.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            49578..50816
                     /locus_tag="OHO85_000998"
     CDS             49578..50816
                     /locus_tag="OHO85_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCQ2670412.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            complement(50957..51283)
                     /locus_tag="OHO85_000999"
     CDS             complement(50957..51283)
                     /locus_tag="OHO85_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCQ2670413.1"
                     /translation="MFRSLFLAAALMAFTPLVANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(51398..52654)
                     /locus_tag="OHO85_001000"
     CDS             complement(51398..52654)
                     /locus_tag="OHO85_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCQ2670414.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASALQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHSMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            52858..53823
                     /gene="glk"
                     /locus_tag="OHO85_001001"
     CDS             52858..53823
                     /gene="glk"
                     /locus_tag="OHO85_001001"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCQ2670415.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHNNPGLLGSGAHLRQTLGHIL"
     gene            54042..54368
                     /locus_tag="OHO85_001002"
     CDS             54042..54368
                     /locus_tag="OHO85_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCQ2670416.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            54390..55637
                     /locus_tag="OHO85_001003"
     CDS             54390..55637
                     /locus_tag="OHO85_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCQ2670417.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            55652..56737
                     /gene="ypdF"
                     /locus_tag="OHO85_001004"
     CDS             55652..56737
                     /gene="ypdF"
                     /locus_tag="OHO85_001004"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCQ2670418.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            56737..57774
                     /gene="ypdE"
                     /locus_tag="OHO85_001005"
     CDS             56737..57774
                     /gene="ypdE"
                     /locus_tag="OHO85_001005"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCQ2670419.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            57799..60294
                     /gene="ptsP"
                     /locus_tag="OHO85_001006"
     CDS             57799..60294
                     /gene="ptsP"
                     /locus_tag="OHO85_001006"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCQ2670420.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(60297..61154)
                     /locus_tag="OHO85_001007"
     CDS             complement(60297..61154)
                     /locus_tag="OHO85_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2670421.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(61167..61901)
                     /gene="ypdB"
                     /locus_tag="OHO85_001008"
     CDS             complement(61167..61901)
                     /gene="ypdB"
                     /locus_tag="OHO85_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCQ2670422.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(61916..63613)
                     /gene="ypdA"
                     /locus_tag="OHO85_001009"
     CDS             complement(61916..63613)
                     /gene="ypdA"
                     /locus_tag="OHO85_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HCQ2670423.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT        14924 a        16175 c        17607 g        15008 t
ORIGIN      
        1 atatgaaagt ttatgcacca cagcgaatat ctctccattc ttagtgatct acctcacctt
       61 ttaaacgcgc ttgccgaatt ttgttattta ctctgacgaa aaattgtcac gatacacgaa
      121 agtttttcac aggcggcgac tcatggataa agaacgcatc attcaggaat ttgtgcccgg
      181 caaacaggtc acactggcgc atctcatcgc gcatcccggt gaggaactgg cgaagaaaat
      241 cggcgttccg gatgcgggcg caattggcat tatgacgcta actcccggcg aaacggcgat
      301 gatcgccggt gatttagcgc tgaaagccgc tgatgtgcat atcggttttc tcgaccgctt
      361 cagcggtgca ttggtgattt atggctcggt gggcgcagta gaagaagcgt tatcacaaac
      421 cgtcagcggt ttaggtcgct tattaaatta tacgctttgt gaaatgacca aaagttaata
      481 cgaggcattc atgaaacgaa ttgcgtttgt gggtagcgtc ggggcaggaa aaacaacgtt
      541 atttaatgcg ttgcagggga attataccct cgccagaaaa acacaggccg tggaatttaa
      601 tgataagggc gatattgata cgccgggtga atattttagc catccccgct ggtatcacgc
      661 cttaattacc acgctacagg atgtagacat gctgatctat gtccatggcg caaatgaccc
      721 ggaaagtcgg ttgcctgccg ggttgctgga tattggcgtc agcaaacggc aaatcgccgt
      781 catcagtaaa acggacatgc cagatgctga cgtcgccgca acgcgaaagt tattgcttga
      841 gacgggtttc gaagagccga ttttcgagct aaacagccac gacccgcaaa gcgtgcagca
      901 actggtggat tatctggcat ctctcaccaa acaggaggaa gcaggtgaaa aaactcatca
      961 cagcgaatga tattcgtgaa gcacacgcgc gcggcgaaca ggcaatgtcc gtggttctgc
     1021 gcgccagcat tattaccccg gaagcccgtg aagtggcgga tctactgggt tttaccatca
     1081 ccgaatgtga tgaatcgatc ccggtaacgg cgtccgtgcc cgccagcgtg ccagccgaca
     1141 aaaccgaaag ccagcgcatc cgcgaaacca tcattgccca gctgccggaa ggccagttta
     1201 ccgaaagcct ggtcgcacag ctgatggaaa aagtgatgaa ggaaaaacag tcgctggagc
     1261 agggcgcaat gcagccgagc ttcaaatcgg tgaccggcaa aggcggcatc aaagtgattg
     1321 atggcagcag cgtcaagttt ggtcgtttcg acggcgcaga gccgcattgc gtgggcttga
     1381 ctgatcttgt gaccggagac gacggcagca gcatggccgc cggttttatg cagtgggaga
     1441 acgcattctt cccgtggacc ctgaactacg acgaaatcga catggtgctg gaaggggagc
     1501 tacatgttcg ccacgaaggc gaaaccatga tcgccaaagc aggcgacgtg atgtttatcc
     1561 cgaaaggctc cagcatcgaa tttggtacga catccagcgt gaaattcctg tatgtcgcct
     1621 ggccggctaa ctggcaatcc ctatgaaaga tttcatcacc gaagcatggc taagagcgaa
     1681 ccatacgctc agcgaaggcg cagagatcca tctccctgcg gacagtcgcc tgacgccctc
     1741 tgcccgggag ttgctggaaa gccgccatct gcgcatcaag tttattgacg agcagggccg
     1801 cctgtttgtt gacgatgaac agcagcagcc gcagcccgtt catgggctga ccagtagcga
     1861 tgaacatccg caggcgtgct gcgaactgtg ccgccagccg gtggcaaaaa agccggatac
     1921 gctgactcac ctgtcggcgg aaaaaatggt cgccaaaagc gatccgcgtc tgggttttcg
     1981 cgcggttctc gacagcacca ttgcgctggc agtgtggctg caaattgaac tggcggaacc
     2041 gtggcagccg tggctggcgg atatccgttc gcgtcttggc aacattatgc gcgccgatgc
     2101 gctgggggaa ccgctgggct gccaggcgat tgtcgggctt tctgacgaag atctgcatcg
     2161 gctttctcat cagccgctgc gctatctcga ccacgatcat ctggtgccgg aagccagcca
     2221 cgggcgcgat gctgcgttgc tcaatctgct acgcaccaaa gtgcgggaaa ccgaaaccgt
     2281 agcggcgcag gtctttatca cccgcagctt tgaagtatta cgcccggaca ttttgcaggc
     2341 gctgaaccgc ctttcgagca cggtctacgt gatgatgatc ctgagcgtaa cgaagcagcc
     2401 gctaacggtg aagcagatcc aacaacgact gggggaaacg caatgattat tgaacgttgt
     2461 cgtgaactgg cgttgcgagc gcccgccaga gtggtttttc cggatgcgtt agaccaacgt
     2521 gtgctgaaag ccgcgcaata tttacatcaa caaggtctgg caacgcccat tctggtcgcc
     2581 aatccgtttg aacttcgtca gtttgcgctc agtcacggcg tggcgatgga cgggctacag
     2641 gtgattgatc cgcatggcaa cctcgcaatg cgggaagaat ttgctcatcg ctggctggcc
     2701 cgcgcgggcg aaaaaacgcc gccggatgcg ctggaaaaac ttaccgaccc tctgatgttc
     2761 gccgcagcaa tggtcagcgc cggtaaagcg gatgtctgta tcgcgggcaa cctctcttcc
     2821 acggcaaatg tgctgcgtgc cggattacgc attatcggct tgcagccagg ctgtaaaacg
     2881 ctctcttcca ttttcctgat gttgccacag tacagcggcc cggcgttggg ctttgccgat
     2941 tgcagcgtgg tgccacagcc gacggcggcg cagctggcgg atatcgcgct tgccagtgcc
     3001 gaaacctggc gcgccatcac cggagaagag ccgcgcgtgg cgatgctgtc gttttccagt
     3061 aacggtagcg cccgtcaccc ctgcgttgcc aacgtccagc aggcgacaga aatcgtccgt
     3121 gagcgcgcac caaagctggt ggttgatggc gagttacagt ttgacgccgc cttcgtgccg
     3181 gaagtggcgg cgcaaaaagc gcctgccagc ccgctacagg gcaaggccaa tgtgatggtt
     3241 tttccgtcgc tggaagccgg aaatattggc tacaaaatcg cacaacgact cggcggatat
     3301 cgtgccgtcg ggccattgat acaaggactt gccgcgccga tgcacgatct ctctcgtggt
     3361 tgtagcgtgc aggaaattat cgagctggcg ctggtggcag ctgtgccgcg tcagacaaaa
     3421 gtgaaccgcg aaagcagttt acaaacactg gttgaatgaa cggtcccgtt ctggaccctg
     3481 ataaagagga aagcacaatg gaagcattag gaatgatcga aacccggggc ctggttgcac
     3541 tgattgaggc ttctgacgcg atggtgaaag ctgcgcgcgt caagctggtt ggtgttaagc
     3601 agattggcgg cggcctgtgt actgccatgg ttcgcggtga tgttgcagcc tgtaaagcgg
     3661 cgaccgatgc cggtgccgca gcggcacagc gtattggcga gctggtatcc gtacacgtta
     3721 tcccgcgtcc gcacggtgac ctggaagaag tcttcccgat cggcctgaaa ggcgatagca
     3781 gcaacctgta atcaccctgt gtgacgtcga ttcgggcgtc acgctctctc ctttttcatt
     3841 ttaccttctg cggagggtag ggggagcttt tacctgaaat acgccacgga ggcgggtatg
     3901 aaactggcag tcgtcactgg acaaattgtt tgtaccgtac gccatcacgg actggcgcat
     3961 gacaaattgc tgatggtgga aatgattgat ccacaaggta atcctgacgg gcaatgcgcc
     4021 gtcgccatcg acaatattgg cgcgggaacc ggggagtggg tgttactggt gagtggcagt
     4081 tccgcccgcc aggcgcataa aagcgaaacg tcaccggtcg atctgtgcgt gattggcatt
     4141 gtcgatgagg tggtgtctgg cggtcaggta attttccaca aataaggcag aacatcatga
     4201 atcaacagga tattgaacag gtggtgaaag cggtactgct gaaaatgcaa agcagtgaca
     4261 cgccgtccgc cgccgttcat gagatgggcg ttttcgcgtc cctggatgac gccgttgcgg
     4321 cagccaaagt cgcccagcaa gggttaaaaa gcgtggcaat gcgccagtta gccattgctg
     4381 ccattcgtga ggcaggcgaa aaacacgcca gagatttagc ggaacttgcc gtcagtgaaa
     4441 ccggcatggg ccgcgttgaa gataaatttg ccaaaaacgt cgctcaggcg cgcggcacgc
     4501 caggcgttga gtgtctctct ccgcaggtgc tgaccggcga caacggcctg actttaattg
     4561 aaaacgcgcc ctggggcgtg gtggcttcgg taactccgtc cactaacccg gcggcaaccg
     4621 taattaataa cgccatcagc ctgattgccg cgggcaacag cgtcattttt gccccgcatc
     4681 cggcggcgaa aaaagtctcc cagcgggcga ttacgctgct caaccaggcg attgttgccg
     4741 caggtgggcc ggaaaactta ctggttactg tggcaaatcc ggatatcgaa accgcgcaac
     4801 gcttgttcaa gtttccgggt atcggcctgc tggtggtaac cggcggcgaa gcggtagtag
     4861 aagcggcgcg taaacacacc aataaacgtc tgattgccgc aggcgctggc aacccgccgg
     4921 tagtggtgga tgaaaccgct gacctcgccc gtgctgctca gtccatcgtc aaaggcgcgt
     4981 ctttcgataa caacatcatt tgtgccgacg aaaaggtact gattgttgtt gatagcgtag
     5041 ccgatgaact gatgcgtctg atggaaggcc agcacgcggt gaaactgacc gcagaacagg
     5101 cgcagcagct gcaaccggtg ttgctgaaaa atatcgacga gcgcggaaaa ggcaccgtca
     5161 gccgtgactg ggttggtcgc gatgcggcga aaatcgcggc ggcaatcggc ctgaacgtgc
     5221 cgcaagaaac gcgtttgctg tttgtggaaa ccactgcaga acatccgttt gccgtgaccg
     5281 aactgatgat gccggtgctg cccgtcgtgc gcgtcgccaa cgtggcggat gccattgcgc
     5341 tggcggtgaa actggaaggc ggttgccacc acacggcggc aatgcactcg cgcaacatcg
     5401 aaaacatgaa ccagatggcg aatgctattg ataccagcat tttcgttaag aacggaccgt
     5461 gcattgccgg gctggggctg ggcggggaag gctggaccac catgaccatc accacgccaa
     5521 ccggtgaagg ggtaaccagc gcgcgtacgt ttgtccgtct gcgtcgctgt gtattagtcg
     5581 atgcgtttcg cattgtttaa ggagtaacag atggcgcacg acgaacaatg gctcacccca
     5641 cgtctgcaaa cggcggcaac gctgtgtaac cagacgcccg ccgcgacgga atctccgctg
     5701 tggctggggg tggatctggg cacctgcgat gtggtgtcga tggttgtcga ccgcgacggt
     5761 cagccggtgg cggtatgcct cgactgggcc gatgtggtcc gcgacggcat cgtctgggat
     5821 ttcttcggcg ctgtcaccat tgttcgtcgc catctcgaca cgctcgagca gcaattcggt
     5881 cgccgtttta gccatgcggc gacttcattt cctccaggta cggacccgcg tatttccatc
     5941 aacgtgctgg agtctgccgg gctggaagtg agccatgtgc tggatgagcc aaccgcagtc
     6001 gcggatctac tgcaactgga taacgcaggc gtagtggata tcggcggtgg cactaccggc
     6061 attgccatcg tgaaaaaggg caaagtgacg tactcggcgg atgaagccac cggcggacat
     6121 cacatctctc tgactctcgc cggaaatcgc cgtatttcgc tggaagaggc ggagcagtac
     6181 aagcgcggtc acggtgacga gatttggcct gcggtgaaac cggtgtacga aaaaatggcg
     6241 gacattgtcg cccggcatat tgaagggcag ggcattactg atttatggct ggcgggcggc
     6301 tcctgtatgc aaccgggcgt ggcagaactg tttcgcaaac aattcccggc gttacaggtg
     6361 catttaccgc agcacagttt gtttatgacc ccgctggcga tcgccagtag tgggcgagag
     6421 aaagcggagg ggctatatgc aaaatgaatt gcagaccgcg ctctttcagg cgttcgatac
     6481 cctgaatctg caacgggtaa aaacatttag cgttccaccg gtgacgcttt gcggtctggg
     6541 cgcggtgagc agttgcgggc agcaagcgca aacgcgtggg ctgaaacatc tgttcgtgat
     6601 ggcagacagc tttttgcatc aggcagggat gaccgccggg ctgacgcgca gcctggccgt
     6661 taaaggcatc gccatgacgc tctggctatg tccggtgggc gaaccgtgca ttaccgacgt
     6721 gtgtgcagcc gtggcgcagt tgcgtgagtc aggttgtgac ggggtgatcg catttggcgg
     6781 cggctcggtg ctggatgcgg cgaaagccgt ggcgttgctg gtgacgaacc cggatagcac
     6841 gctggcagag atgtcagaaa ccagcgttct gcaaccgcgc ttgccgctga ttgccattcc
     6901 aactaccgcc ggaaccggct ctgaaaccac caatgtaacg gtgattatcg acgcggtgag
     6961 cgggcgcaag caggtgttag cccatgcctc gctgatgccg gatgtggcga tcctcgacgc
     7021 cgcattgacc gaaggtgtgc cgtcgcatgt cacggcgatg accggcattg atgcgttaac
     7081 ccatgccatt gaagcataca gcgccctgaa cgctacaccg tttaccgaca gtctggcgat
     7141 tggtgccatt gcgatgattg gcaaatcgct gccgaaagcg gtgggctacg gtcacgacct
     7201 tgccgcgcgc gagagcatgt tgctggcttc atgtatggcg ggaatggcgt tttccagtgc
     7261 gggtcttggg ttgtgccacg cgatggcgca tcagccgggc gcggcgctgc atattccaca
     7321 cggtctggcg aacgccatgt tgctgccaac ggtgatggaa tttaaccgga tggtttgtcg
     7381 cgaacggttt agtcagattg gtcgggcact gcgaactaaa aaatccgacg atcgtgacgc
     7441 tattaacgcg gtaagtgagc tgattgcgga agttgggatt ggtaaacgac tgggcgatgt
     7501 tggcgcgaca tctgcgcatt acggtgcatg ggcgcaggcc gcgcaggaag atatttgtct
     7561 gcgcagcaac ccgcgtaccg ccagcctgga acagattgtc ggactgtacg cggcggcgca
     7621 ataaatgccg gatgcgacgc ttgccgcgtc ttatccggcc tacgggattg cacatgtagg
     7681 gcggataagg cgtttacgcc gcatccgcca ataaataaca actaacaggg agtaaaggcg
     7741 atgggaatta acgaaatcat catgtacatc atgatgttct ttatgctgat agctgccgta
     7801 gacaggatcc tgtcgcagtt cggcggttct gctcgtttcc tcggtaagtt cggtaaaagt
     7861 atcgaaggat caggcggtca gttcgaagaa ggctttatgg caatgggcgc tctgggtctg
     7921 gcgatggtcg gtatgaccgc gctggcaccg gtactggctc acgtactcgg gccggtaatt
     7981 atcccggttt acgaaatgct cggcgcaaac ccgtcaatgt tcgccggaac actgctggcg
     8041 tgcgatatgg gcggcttctt cctcgccaaa gagctggcgg gcggcgacgt agcagcgtgg
     8101 ctatactctg ggttaattct cgggtcgatg atggggccaa cgattgtgtt ttccattccg
     8161 gtggcgctcg gcattatcga accttctgat cgtcgttatc tggcgctcgg cgtgctggcg
     8221 ggcattgtga ccattccgat tggctgtatt gctggtggtc tggttgctat gtactccggt
     8281 gtgcagatca acggtcagcc ggtggaattc actttcgccc tgatcctgat gaacatgatc
     8341 ccggtgatca ttgtcgcgat tctggtggcg ctggggctga aattcatccc ggaaaaaatg
     8401 atcaacggct tccagatctt tgccaaattc ctcgttgcat tgatcaccct cggtcttgcc
     8461 gctgcggtag tgaaattcct ccttggctgg gaactgatcc ccggactgga tcctatcttt
     8521 atggcccctg gcgataaacc cggtgaagtg atgcgcgcca ttgaagttat cggttctatc
     8581 tcctgcgtgc tgttaggggc gtatccaatg gtgctgctgc tgactcgctg gtttgaaaaa
     8641 ccgctgatga gcgtcggtaa agtactgaat atgaacaaca tcgcggcagc cggcatggtg
     8701 gcaacgcttg ccaacaacat cccgatgttc ggcatgatga agcagatgga tacccgcggc
     8761 aaagtcatca actgcgcctt cgccgtttcc gctgctttcg ccctgggcga ccacttaggc
     8821 ttcgccgctg ccaacatgaa cgccatgatc ttcccgatga ttgtcggcaa gttgatcggc
     8881 ggcgtaacgg cgattggcgt ggcgatgatg ctggtgccaa aagaagacgc gaccgcgact
     8941 aaaaccgaag cggaggcaca atcgtgaaca ctcgccagct attgagcgtc ggtatcgata
     9001 tcggcaccac caccacccag gtgattttct cccacctgga actggttaac cgtgcggcgg
     9061 tgtcgcaggt gccgcgctac gaattcatta aacgcgaaat tagctggcaa agcccggtat
     9121 tctttacccc tgtcgataaa cagggtggtt taaaagaagc ggaactgaaa tcgctgattt
     9181 tagagcaata tcaggctgcg ggcattgcgc cggaaagcgt tgattctggt gccatcatca
     9241 tcaccggtga aagcgcgaaa acccgcaatg ctcgcccggc ggtgatggcg ctctctcagt
     9301 cgctgggcga ttttgtcgtt gccagcgccg ggccgcacct cgaatccgtg atcgccggtc
     9361 acggagccgg ggcacaaacc ctttctgaac aacggctgtg tcgggtactg aatatcgaca
     9421 tcggcggtgg caccgcgaac tacgccctgt tcgatgccgg aaaaatcagc ggcactgcct
     9481 gtctcaacgt cggtggtcgc ctgctggaaa ccgacagcca ggggcgcgtg gtttacgctc
     9541 ataaaccggg gcagatgatt gtggatgagt gcttcggtgc aggcactgac gcccgttcgc
     9601 tgaccggcgc gcagctggtg caggttaccc ggcggatggc ggcgctgatt gtcgaagtga
     9661 ttgacggaac gctttcgccg ctcgcgcagg cattgatgca aaccggtttg ctgcccgcag
     9721 gtgttacgcc cgaaatcatt acgctttctg gaggcgtggg cgaatgttat cgccaccagc
     9781 ccgccgaccc gttctgtttt gccgatattg gcccacttct ggcaacggcg ctgcatgacc
     9841 atccgcgcct gcgtgagatg aatgtgcagt ttccggcgca aaccgtacgc gccacggtga
     9901 ttggcgcggg tgcacatacc ctttcgctct ctggcagcac aatctggctg gagggcgtac
     9961 aactgccgct gcgcaatttg ccggtggcga tcccgattga tgaaacggat ctggtgagtg
    10021 cctggcaaca ggcgctgctt cagctggatc ttgatcccaa aactgacgcg tacgtgctgg
    10081 cgcttcccgc ctcgctgcct gtgcgttacg ccgcggtact gacggtcatc aacgcgctgg
    10141 tcgatttcgt cgcgcgtttt ccgaatccgc atcccctgct ggtggtggcc gggcaggact
    10201 ttggtaaagc tctgggcatg ttgttgcgcc cacagctaca acaactcccg ttggcagtca
    10261 ttgacgaagt gattgtccgc gcgggggact atatcgacat tggtacgcct ctttttggcg
    10321 gatcggttgt gccggtgacg gtgaaatcac tcgcatttcc ttcctgaggg aacgacttat
    10381 gaaactaaag accacattgt tcagcaatgt atatcagttt aaggatgtaa aagaggtgct
    10441 ggctaaagcc aacgaactgc gttcggggga tgtgctggca ggcgttgcag cggcaagttc
    10501 acaggagcgc gtggcggcaa agcaggtgtt gtcggaaatg accgtagcgg acatccgcaa
    10561 taatccggtg attgcctatg aagatgactg cgtgacgcgg ctgattcagg acgatgttaa
    10621 cgaaacggcc tacaaccaga ttaaaaactg gagcatcagc gaactgcgtg agtatgtgct
    10681 gagcgatgaa accagcgtgg acgacattgc ctttacccgc aaagggctga cctcggaagt
    10741 ggtcgcggcg gtagcgaaga tttgctccaa cgcggacctg atctacggcg cgaagaaaat
    10801 gccggtaatc aaaaaggcca ataccaccat tggtattccg ggcaccttca gcgcccgttt
    10861 gcagccaaat gacacccgtg acgacgtgca aagtatcgcc gcgcaaatct acgaagggct
    10921 ttccttcggg gtgggcgatg cggtgatcgg cgttaacccg gtgactgacg acgtggaaaa
    10981 cttaagccgc gtgttggaca ccatttacgg cgtgatcgac aaattcaaca tcccaactca
    11041 gggctgcgta ctggcgcacg tcaccaccca gatcgaagcg attcgtcgcg gcgcgcctgg
    11101 cggactgatt ttccagagta tctgtggcag cgaaaaaggg ctgaaagagt ttggcgtgga
    11161 actggcgatg ctcgacgaag cgcgcgcagt gggtgcagag ttcaaccgta tcgccgggga
    11221 aaactgcctc tacttcgaaa ccggacaagg ctctgcgcta tccgctggcg ctaacttcgg
    11281 cgctgaccag gtgacgatgg aagcacgtaa ctatgggctg gcgcgtcatt acgatccgtt
    11341 tatcgtcaac accgtggtcg gctttattgg gccggagtat ctctacaacg accgccagat
    11401 tatccgtgct ggcttagaag atcactttat gggcaagctg agcggcatct ctatgggctg
    11461 tgactgctgt tacaccaacc acgctgacgc tgaccagaac ctcaacgaaa acctgatgat
    11521 cctgctcgcc accgcaggct gcaactacat catggggatg ccgctgggtg atgacatcat
    11581 gctcaactac cagaccaccg cattccacga taccgccact gtgcgtcagt tactcaacct
    11641 gcgtccgtca ccggagtttg aacgctggct ggaaagcatg ggcattatgg caaacggtcg
    11701 cctgaccaaa cgggcgggcg atccgtcact gttcttctga tgacgcggag ataacacatc
    11761 atggatcaaa aacagattga agaaattgta cgcagcgtga tggcgtcaat gggacaaacg
    11821 gcccccgcgc cgtcagaagc aaagtgcgcc accaccaact gtgcggcacc ggtgacctcg
    11881 gaaagctgtg cgctggattt aggttccgct gaagcaaaag cgtggattgg tgttgaaaac
    11941 ccgcatcgcg cagacgtatt aacagaactg cgccgcagca ccgtggcccg cgtttgtacc
    12001 ggtcgtgccg gtccgcgtcc acgtacccag gcgctgctgc gtttcctggc cgatcactct
    12061 cgttcgaaag ataccgtact gaaagaagtg ccggaagagt gggtgaaagc gcagggcttg
    12121 ctggaagtac gctctgaaat tagcgacaaa aatctctacc tgactcgccc ggatatgggc
    12181 cgccgcctgt gtgcagaagc tgttgaagcg ctgaaagcac agtgtgttgc taatccggac
    12241 gtacaggtcg ttatttctga tggcctgtca actgatgcca tcaccgtgaa ctacgaagag
    12301 atcctgccgc cgctgatggc gggcctgaaa caggccgggc tgaaagtcgg cacgccattc
    12361 tttgttcgtt atggtcgcgt gaagattgaa gatcagatcg gtgagatcct cggcgcgaaa
    12421 gtggtgatcc tgctggtggg ggaacgtccg gggttagggc agtcagaaag cctctcctgc
    12481 tacgccgttt actcgccgcg tatggcgacc accgttgagg ccgatcgcac ctgtatctct
    12541 aacattcacc agggcggcac gccgccggtt gaagccgcag ccgtcattgt ggatttggcg
    12601 aaacgtatgc tggagcagaa agcatccggc atcaacatga cccgataagg aggcatcatg
    12661 ccagctttag atttgattcg accgtcggta accgccatgc gggtgattgc ctccgttaac
    12721 gctgaatttg cgcgtgaact aaaattgccg ccgcatattc gtagcctcgg gctgatttct
    12781 gctgattccg atgacgtcac ctacattgcc gctgatgaag cgaccaagca ggcgatggtg
    12841 gaagtggtat atggtcgctc gctgtatgcg ggcgctgcac acggcccgtc accgactgcc
    12901 ggtgaagtgc tgattatgct tggtgggcca aacccggcgg aagtgcgtgc cggtctggat
    12961 gcgatggttg cacatattga aaatggtgca gcattccagt gggccaatga cgcagaaaac
    13021 accgccttcc tggcgcatgt ggtttcgcgt accggttctt atctctcatc aaccgccggg
    13081 atcacgcttg gcgatccgat ggcgtatctg gtggcaccgc cgctggaagc gacctatggc
    13141 attgatgcag cgttgaaatc tgccgacgta cagctggtga cctatgtccc gccaccgtct
    13201 gaaaccaact actcggcagc atttttaacc ggtagccagg ccgcgtgtaa agcagcctgt
    13261 aacgccttta ccgatgcagt gctggaaatc gcgcgtaatc caatccagcg tgcgtaacgg
    13321 aggttgccga tgatcaatgc actgggattg ctggaagtgg acggcatggt cgccgcgata
    13381 gatgcagcgg atgccatgct caaagcagct aacgttcgtc tgctcagtca cgaagtgctt
    13441 gaccctggtc gcttaacgct ggtggtggaa ggcgatctgg cggcgtgtcg tgcggcgctg
    13501 gacgcaggtt gtgctgccgc gatgcgtacc gggcgtgtca tcagccgcaa ggagatcggt
    13561 cggccagacg atgacaccca gtggctggtt actggcttta accgccagcc gaagcaaccc
    13621 gtaagggaac ccgacgcgcc agttatcgtc gcggaatctg ctgacgagtt gttggcgctg
    13681 ttaacatcag tacgtcaggg aatgacggca ggagaagtgg ctgcccactt tggctggccg
    13741 ctggaaaaag ccagaaatgc gctcgaacag ctcttttctg ccgggacgtt acgtaaacgc
    13801 agtagtcgtt atcgtctcaa gccccattaa cctgtcggag gtgccgggtg tcctacaaca
    13861 cccggcatta acatcatgaa aaagacccgt acagccaatt tgcaccatct ttatcatgaa
    13921 cccttacccg aaaacctgaa gctcacgccg aaggtcgaag tggataatgt tcatcaacga
    13981 cagacaacgg atgtctatga acatgcttta acgattaccg cctggcagca gatttacgat
    14041 cagctgcatc cgggcaagtt tcatggtgaa tttacggaaa ttctactcga tgatattcag
    14101 gtttttcgtg aatacaccgg tctggcgctg cgtcagtcgt gcctggtctg gccgaactcg
    14161 ttctggtttg gcattccggc gacgcgcggt gagcagggat ttatcggttc gcaatgtctg
    14221 ggaagcgcgg aaatcgccac ccgccctggt ggcactgaat ttgaactgag cacgccggat
    14281 gattacacga tcctgggcgt ggtgctttct gaagatgtca tcacccggca ggctaacttt
    14341 ttgcataacc cggatcgggt attacatatg ctgcgtagcc agtcggcgct ggaagtgaaa
    14401 gagcagcata aagccgcgct gtggggcttt gtccaacagg cgctggcgac gttttgcgag
    14461 aacccggaaa atctccatca gcctgcagtg cgaaaagtgc tgggggataa tttgctaatg
    14521 gcgatggggg cgatgctgga agacgcgcaa ccaatggtga cggcggaaag catcagtcat
    14581 cagagttacc gtagattact ttcccgcgcc cgtgaatatg tgctggaaaa tatgtctgag
    14641 ccggtgacgg tgctggactt gtgtaatcaa ctgcatgtca gtcgccgcac gctacaaaac
    14701 gcgtttcacg ctattttagg cattggccca aacgcgtggc tgaaacgcat tcgcctgaac
    14761 gccgtacgcc gcgaactgat aagcccgtgg tcgcaaagca caacggtaaa agacgccgcc
    14821 atgcagtggg gattctggca tctggggcaa tttgccacgg attaccagca gctgtttgcc
    14881 gagaagccgt cgttgacgtt gcatcagcgg atgcgggaat gggggtgagg gagttacacc
    14941 caatccctga ccttaataaa ctcacttaac gccgcttctg ggctgccatc ttttggctga
    15001 taatcatact cccagcgtac cagtggcggc attgacatca ggatcgactc ggtgcgcccg
    15061 ccagtttgca ggccaaacag agtgccgcga tcccagacca gattgaactc gacataacga
    15121 ccgcgacggt agagctgaaa attgcgctcg cgctcgccgt aggccatcgc tttacgtcgc
    15181 tctacaattg gtaaataagc gtcggtgtag cctttgccta ccgcctgcat aaaggcaaaa
    15241 cagtggtcga aatctggcgt gttcagatca tcaaagaaca gcccgccaat accgcgctgt
    15301 tcgttgcgat gtttgaggta gaagtaatcg tcgcaccact ttttgtaacg gggataaacg
    15361 tcttcaccaa atggcaggca caggtcacgg gcggtgcggt gccagtgaat ggcgtcttct
    15421 tcaaaaccat agaaaggggt taaatcgaag ccgccgccaa accaccagac gggctctgca
    15481 cccggttttt cggcaataaa aaaacgcaca ttcgcgtggc tggtgggaac atacgggtta
    15541 tgcggatgca ctaccagtga aacgcccatc gcctcgaaac tgcgcccggc aagttccggg
    15601 cgatgagcgg tggcggaagc tggcatcgcc tcgccgtgga cgtgagaaaa gttgacgcct
    15661 gcctgttcga aaacaccacc attacgcaac acccgactac gcccgccgcc gccagcttcg
    15721 cgctgccaac tatcttcgac aaattctgcg ccatcgacgg cggtcagctg ctgacaaatc
    15781 gtatcctgaa ggttgagcag aaactgttta acctggtgtg cgtcgggttt cataacttat
    15841 cgctttttcg aatgtgcttt ctggttgtcg aaccagtgga aataactgat cacgccttca
    15901 gcaatcgctg tggcgatttt ctgacgaaac gccgccgtgc ctaacagccg ctcttcttcc
    15961 gggttggtga taaacgaggt ttccaccagc accgaaggaa ccgacggcga tttcaacacc
    16021 acaaacgcgg cttgttcggt gttgcggctg tgcagtttat gcaccggctt aatctttttc
    16081 agaatatgcg agccgagcgt caggctgttt ttaatagtat cggtttgcac cagatcaaac
    16141 agcacttgct gcaataggtg atccttgtca gtcgcctttt taccggcaac ttcatcggcg
    16201 cggttttcgc gttcagacag gtatttcgcc attgcgctac ttgccccacg gttagagagg
    16261 gcaaataccg aagcaccggc agctttcggg ttggtaaagc catcggcatg aattgacata
    16321 aacagatctg cgccatgttt atgggcgatt tcaacgcgat cgtaaagtgg gataaacgta
    16381 tcgccagaac gcgttaaacg cgcatcaatc ccatgattac gcaaaatgga acggacgttt
    16441 ttagcaatcg ccagcaccac atgtttttct ttcgaaccgt tgcgtccgat cgctccggta
    16501 tcaattccgc cgtggcctgg atcgagaaca acgacacgtt tgccgccaga ttttttggct
    16561 ttcggcttgc tgtgtccgtt gctggttttt aaaggttcgt ctttggcgat ggcttgcgac
    16621 attcctgaca acgtcagggc agccaaaccg gctttcagca cctggcggcg cgaagtgagt
    16681 gtttttagtg gtttaaaagt gctcatacgg cctgagttgt aataataaag ttccagatgt
    16741 tatatcgtat cgcgtaatcc gttacgacgg tttgatgttg agaattgttt tacttttcat
    16801 ttcaatagtt gacaactccc cattatgcca ctttttcaac cgattttcgt cagatattgg
    16861 ctaattcgac cgttcacgcc ataatcagcc ttttaacccg caaaaaggta acgaatacca
    16921 tggagatacg cgtatttcgc caggaagatt tcgaagaggt catcaccctt tgggagcgtt
    16981 gcgacttgct gcgtccgtgg aacgatccgg aaatggacat cgagcgtaag atgaaccatg
    17041 acgtcagttt gtttttggtc gctgaggtaa acggtgaagt ggtcggaacg gtgatgggcg
    17101 gttatgacgg gcatcgcggg tctgcttatt atcttggcgt gcatccagag tttcgtgggc
    17161 gtgggattgc caatgcgttg cttaatcggc tggagaaaaa gctgattgct cgtggctgcc
    17221 cgaaaattca gatcaatgtg ccggaagata acgacatggt gctcggaatg tatgaacgcc
    17281 tgggatatga acacgccgac gtgctgagtc tgggtaagcg tttgattgaa gatgaagagt
    17341 actgagttcc atcctgtcca ttatgatgcg cacggacgcc tgcgtttacc cttgctcttc
    17401 tggcttgtgc tattgcttca ggcgcgaacc tgggtgctgt ttgtcattgc cggtgcgtcg
    17461 cgtgagcagg gtactgcgct gctaaatctg ttttatcccg atcacgataa tttctggctg
    17521 gggctgattc ctggcattcc ggcggtgctg gcgtttttgt tgagcggtcg gcgggctacg
    17581 tttcctcgca cctggcgtgt actctatttt ctgttgctgt tggcgcaggt ggttttactt
    17641 tgctggcaac cgtggctgtg gctgaacggt gaatccgtta gcggtatcgg tctggcgctg
    17701 gtagtggcgg atattgtggc gttaatctgg ctgctgacca atcgacgttt acgcgcttgt
    17761 tttaatgaag taaaagaata acggcacttt ttggtgaatt tgcactccaa gcaacgttat
    17821 tgaataacca aaggaagtga catgaaatcg ctgcgtttaa tgttatgcgc tatgccattg
    17881 atgctgaccg gctgttccac gatgtcgtca gttaactggt ctgccgctaa cccgtggaac
    17941 tggtttgggt cgtccaccaa agtgagcgag cagggcgttg gtgaattaac ggcgtccaca
    18001 ccactgcaag aacaagccat tgccgatgcg cttgatggcg attatcgcct gcgcagcgga
    18061 atgaaaaccg cgaacggcaa cgtggtgcgc ttttttgaag tgatgaaggg cgacaacgtg
    18121 gcgatggtga ttaacggcga tcaaggcacg atcagtcgca ttgacgtgct ggatagcgat
    18181 attcctgctg acaccggtgt taaaatcggt acaccgttta gcgaccttta cagcaaagca
    18241 tttggcaatt gccaaaaagc tgatggtgat gataatcgtg ccgtcgaatg taaagccgaa
    18301 ggcagtcaac atattagcta ccagttcagc ggggaatgga gtggtcctga agggttaatg
    18361 ccttcggacg ataccctgaa aaactggaaa gtcagtaaaa ttatctggcg gcgttaattt
    18421 gcgtctgaac aaaccgccgc cgcgaaaaaa cacgtaaaat agcgctcaac aatgccacgg
    18481 attgcgtggc attattattt tcaggaggaa caatgtctca ggttcagagt ggcattttgc
    18541 cagaacattg ccgcgcggcg atttggatcg aagccaacgt gaaaggggaa gttgacgccc
    18601 tgcgtgcggc cagtaaaaca tttgccgaca aactggcaac ttttgaagcg aaattcccgg
    18661 acgcgcatct tggtgcggtg gttgcctttg gtaacaacac ctggcgcgct ctgagcggcg
    18721 gcgttggggc agaagagctg aaagattttc cgggctacgg taaaggcctt gcgccgacca
    18781 cccagttcga tgtgttgatc cacattcttt ctctgcgtca cgacgtaaac ttctctgtcg
    18841 cccaggcggc gatggaagcc tttggtgact gcattgaagt gaaagaagag atccacggct
    18901 tccgttgggt tgaagagcgt gacctgagcg gctttgttga cggtacggaa aacccggcgg
    18961 gtgaagagac gcgtcgcgaa gtggcggtta tcaaagacgg cgtggatgcg ggcggcagct
    19021 atgtgtttgt ccagcgttgg gaacacaacc tgaagcagct caaccggatg agcgttcacg
    19081 atcaggagat gatgatcggg cgcaccaaag aggccaacga agagatcgac ggcgacgaac
    19141 gtccggaaac ctctcacctc acccgcgttg atctgaaaga agatggcaaa gggctgaaga
    19201 ttgttcgcca gagcctgccg tacggcactg ccagtggcac tcacggtctg tacttctgcg
    19261 cctactgcgc gcgtctgcat aacattgagc agcaactgct gagcatgttt ggcgataccg
    19321 atggtaagcg tgatgcgatg ttgcgtttca ccaaaccggt aaccggcggc tattatttcg
    19381 caccgtcgct ggacaagttg atggcgctgt aagcgtttct gattcattga ttaaaggcca
    19441 ggcagtaaca ttactgactg gcctttttat tacttttgct ttaacgccgc atacacctga
    19501 tcaactaccc aaccatacgt tgcaatcggc agctgaccgc tttcgaacat attgggattc
    19561 ttttgcggat cggcaaccgg cgaatgtggc ttgttgctta acatcacaat cgccatatga
    19621 ttcaccggat cgataacggt caccgttccg gtccagccag tgtgaccgta ggtttgcggg
    19681 cttgccagcg tgccaaacgt cggcgtcatg gtggcattac cattcacgcg ccagccgagg
    19741 ccaaaagtgg catcttcctt agagctggtg gtgaacatct tcaccgtttc cgcactgaac
    19801 agctgcacat cgccatagcc cccgccgttc agcatcgttt gcattaacac cgcaatatcg
    19861 ccggtattgg aaaacaaacc tgcgtgcccg gaaacgccgc ccatcgaata aaaggctttt
    19921 tcatcgtgca cctgacccca gagagtggag gtgcggatat tcgggaaatg gatcacgcca
    19981 tcgcgggtat tgccgttcaa ttccgtggcg gcaatttgtt gcggtttaaa gcctttcagc
    20041 agcgggttaa ataccgtatg tgtcaggccg agcgggcgat aaatcgactc ttcaacatag
    20101 cggtcaagcg gctgaccggt gaccgactcg acgataaatc caagcagcat ataatcgaca
    20161 tcactgtaaa tatgcttgct gccgggctga tactctagcg gcgtgcgctt gatcatctcc
    20221 agcgtctggc ctttatcctg ggaatataac gcgcccgcga ccgctttatt cgggtattgc
    20281 ggatctgccg ggaaaccgcc gctgtgatgc agcaggtcag aaatccgcag ggtgtttttg
    20341 cctttaatgg tgtcatttgg actatcggca aaccccggaa tatatttcgc aatccgatcg
    20401 tcaggatgca gtttgccttc gctcatcagc ttttgcaggg cgaagttcgt ggcgtacatt
    20461 ttggtgtttg aggccaggtc atacagcgtc ccggtggtgg ctttgacagg ctgttccatc
    20521 aacacgctgc catcgtactt tttcgccgct ccccaggctt tgcgatacac aatctgatta
    20581 tctttaatga tcagcaggtt tacgccggga taaccggcgt ccacttgctg gctaatccag
    20641 cgatccatct ggttaagccg ttcgacgttg aaccctgctt tctctggcga actttctgtc
    20701 agaacagggt attttgcggc gctgacgcta caactaacag ccagcagaga aagatagagc
    20761 attgtccgtt tcatgatatc agtccagatt gacgttacgg cagccaaaga gcgtggtgaa
    20821 aataaacccg caaacccatg ccaccagaat cccgccagca taaaccgcca ttgccggaag
    20881 gatgccttgt gcggaagtca tcagcggcag cgccaccaga ccagacggcc caaaggcgct
    20941 gtttaagccc atcggcagac cccaccaggc tatcaggccg ataaacaaac cgcccgctgc
    21001 gccgcctaaa caggcggtaa taaacggttt catgcggggc agggtgacgc cgtaaatcaa
    21061 cggttcacca acgcccagca gaccgggaat aatcgccccg cgtacctgac tgcgtaatgc
    21121 actgtgcggt tgcgcccgcc agtagagtgc cagcgccgcg cccacctggc ccgcgcctgc
    21181 cattgaaagg atgggaaata agctgttgaa tccctggctg tccattaacg cgaggtaaac
    21241 aggaataaag ccctgatgca cgccaaatac cacggcgatc aggaacagcc cggctaaaac
    21301 cgcacaaccg aacggattac tgttcaggtg cataaacagc catgacatac cttcgaataa
    21361 ccagccaccc agcggcataa tgatcaggta cgcaagcgta gcggtgatca gcagggtgat
    21421 taacgaggtc agcagcatgt cgagatcgtc cggcataaag cggcgcacca tgccttcaat
    21481 gcgggcgcag gcccacgcgg caatcagcac gccgataata ttgccgcgcg gatcgatggg
    21541 cagaccaaag aaatcgtgaa aaccggcgta gtaaccggtg gttgcagcgg ggttataacc
    21601 gagcaaaaag agtgcggcaa taattgcgcc attgacgccc gtgccgccaa atgcctgggc
    21661 ggcgttatag cccaccagaa tcaccaggaa agtgaacaaa cctttgctga acaccttcat
    21721 aaaattcagc gcgtcgggta gcgtgccctg agcatctgcc ggaacgtgca tcaccgtggc
    21781 aattaacgtc gctatcccca gcagcagacc ggcggcaata aaaccgggga tcagcggcgt
    21841 gaagatggtg gcgaatttgg cgagaaattg ttgcacgccg gaggtttgtt tggcttttaa
    21901 ctgacgtttg ttctgggcgg cgatttctgc ggcatcctgt accggtgcct cgcccagcag
    21961 ctcgctcatc gcttttgcgg cacgatgcgc tttaccaggc ccaaaaacga cctgcacctg
    22021 gtcgctggtc aaaatgacgc ccttcacgcc ttccagcgtt ttgatattgg gatcaaccag
    22081 tgaactgtca tgtacaccca gacgcaggcg cgtcatacag ttaccacaac tggcgatatt
    22141 tcccggtccg ccgacacggg taagaatggt gttcagaagt tcactgctga tctctttggc
    22201 catgggttat tccttgtcta aaacctgacg aataaagccg ccgtgttgat ccaggcgttt
    22261 tttagcttct gcggcatcga gatttttcag caccatcaca atggccgttt tacagttgcg
    22321 ctcgcaggca attaacgccg cttccgcttg ttcggcatta catccggtgg cgtttttaac
    22381 gatattgacc tggcgcacat gcagtttttc gttggtggcg accacatcga ccatcaggtt
    22441 gccgaacact ttgccagatt taatcatcag cccggtggaa agcatattga gcaccagttt
    22501 ctgcgctgtg cctgctttca ttcgcgaaga accagtgacc acttcggcac ctacaatcgg
    22561 tgtaatggca aactcggcgg tggtggaaac ggcactcccc ggattacagg aaattcccac
    22621 tgtgcggcag ccgagctggc gtgcgtattc cagtccggca atcacatacg gcgtgcgacc
    22681 gctggcagca atgccaacca ccacatcctg tgccgttaaa ttaatatttt tcagatcgtt
    22741 aacaccacct tcccggctat cttcagcgcc ttccaccgcg tgctgaatgg catattcgcc
    22801 tccagcaatc aaaccaacca ccagacccgg tttcacgccg taggtgggcg gacattcgct
    22861 ggcatccaga atccccagac gaccggatgt tcccgcaccg agataaatca gacgaccgcc
    22921 gccgctgacc tgggcgtgga taacatcgat cgccgcggcg atatccggca ggacgcgctc
    22981 aacggcaagc ggtacggttt tatcttcatc gttgataatc cggcacattt ccagcgtcga
    23041 tacgcggtca atttcagccg aggcggtgtt cgagccttca gtaatcatct tttctagttg
    23101 catcggtggt gaccttacta tttacgatca aataattgca gatgtatgga atcatatatc
    23161 ccgaattgac agacttaaag gaatcattga gtctacactc gcagagtgtg aagagcgtca
    23221 taagaactta agcggaattt atgctgccag cccaggagag tggaagatgt tgtacctgac
    23281 gaaaataagc aatgcgggga gtgaattcac cgaaaacgag caaaaaatcg ctgactttct
    23341 gcaggccaac gtgagtgagc tgcaatccgt ttcttcacgg cagatggcga aacagctcga
    23401 cattagccag tcgagcatag tgaaatttgc ccaaaaactc ggcgcgcaag gatttactga
    23461 attgcgtatg gcgttaatag gcgaatacag tgccagccgg gagaaaacga atgccacggc
    23521 gctgcacctg cacagttcga ttaccagtga tgattcgctg gaagtgattg cccgcaaatt
    23581 gaatcgcgaa aaagagctgg cgctggagca aacctgtgcg ttgttcgact acgcgcggct
    23641 gcaaaaaatc attgaggtca tcagtaaagc accgtttatc cagataacgg gcctgggcgg
    23701 atcggcgctg gtggggcgcg atctgtcgtt caaactgatg aaaattggct atcgcgtggc
    23761 ctgcgaagcc gacacgcacg tacaagccac cgtttcccag gcgttgaaaa aaggcgatgt
    23821 acaaatcgct atctcttaca gcggcagtaa aaaagagatt gtcttatgcg cagaagcggc
    23881 acgaaagcag ggggcaacgg tgattgccat tacttctctg gctgattctc cgctgcggcg
    23941 gctggcgcat ttcacgctcg ataccgtttc cggtgaaacc gaatggcgta gctcgtcgat
    24001 gtctacccgc acggcgcaaa attctgttac cgatttactg tttgttggtc ttgtgcaact
    24061 caatgatgtg gagtcattga aaatgattca gcgcagcagt gagctaacac aacgcctgaa
    24121 atgatgcata aagcagcgac tggattgaga ttttcctgaa ttagtgagct gatccgcagc
    24181 aatattttgt ttatcctgta ttttcagagg gaatggagtg taacgctctg tattaacaag
    24241 gagagcatta aaatgggtaa actcacgggc aagacagcac tgattacggg cgcattgcag
    24301 ggaattggcg aaggaattgc cagaactttt gcgcgtcatg gcgcaaacct aatcttgctg
    24361 gatatctccc ctgagatcga aaagctggcg gacgaactgt gtggtcgtgg tcatcgctgt
    24421 acggcggttg tcgccgatgt gcgtgacccg gcgtcggtag ccgcagctat caaacgcgcg
    24481 aaggaaaaag aagggcgcat tgatatcctg gtgaataacg caggcgtttg tcgtctgggc
    24541 agtttcctcg atatgagcga tgacgatcgc gatttccata ttgacatcaa tattaaaggc
    24601 gtatggaacg tcacgaaggc ggtgctgccg gagatgattg cccgcaaaga tggtcgcatt
    24661 gtgatgatgt cttcggtcac tggcgatatg gtggccgatc ctggcgaaac ggcgtacgcc
    24721 ttaacgaaag cggcgattgt tggcctgaca aaatcgctgg cggtggagta cgcgcagtct
    24781 ggtattcgcg ttaacgccat ttgcccggga tacgtgcgca caccaatggc ggaaagcatt
    24841 gcccgccagt cgaacccgga agatccagag tcggtgctga ctgaaatggc gaaagcaatc
    24901 ccgatgcgtc gcctcgccga tccgctggaa gtcggcgaac tggcggcctt cctcgcatcg
    24961 gatgaatcca gctatttaac cggtacacag aatgtgattg atggcggcag cacactgccg
    25021 gagacggtta gcgtcggtat ctgattcacc tctgtttcct ccctgcattt gtggggagga
    25081 tttcgtttta aacccggttc ttattccctt ttcaacttcc aaatcaccaa acggtatata
    25141 aaaccgttac tcctttcacg tccgttataa atatgatggc tattagaaag tcattaaatt
    25201 tataagggtg cgcaatggcc gttaacttac tgaaaaagaa ctcactcgcg ctggtcgctt
    25261 ctctgctgct ggcgggccat gtacaggcaa cggaactgct gaacagttct tatgacgtct
    25321 cccgcgagct gtttgccgcc ctgaatccgc cgtttgagca acaatgggca aaagataacg
    25381 gcggcgacaa actgacgata aaacaatctc atgccgggtc atcaaaacag gcgctggcga
    25441 ttttacaggg cttaaaagcc gacgttgtca cttataacca ggtgaccgac gtacaaatcc
    25501 tgcacgataa aggcaagctg atcccggccg actggcagtc gcgcctgccg aataatagct
    25561 cgccgttcta ctccaccatg ggcttcctgg tgcgtaaggg taacccgaag aatatccacg
    25621 attggaacga cctggtgcgc tccgacgtga agctgatttt cccgaacccg aaaacgtcgg
    25681 gtaacgcgcg ttatacctat ctggcggcat ggggcgcagc ggataaagct gacggtggcg
    25741 ataaagccaa aaccgaacag tttatgaccc agttcctgaa aaacgttgaa gtgttcgata
    25801 ccggcggtcg tggcgcaacc accacttttg ccgagcgtgg cctgggcgat gtgctgatta
    25861 gcttcgaatc ggaagtgaac aacatccgta aacagtatga agcgcagggc tttgaagtgg
    25921 tgattccgaa aaccaacatt ctggcggaat tcccggtggc gtgggttgat aaaaacgtgc
    25981 aggccaacgg tacggaaaaa gccgccaaag cctatctgaa ctggctctat agcccgcagg
    26041 cgcaaaccat catcaccgac tattactacc gcgtgaataa cccggaggtg atggacaaac
    26101 tgaaagacaa attcccgcag accgagctgt tccgcgtgga agacaaattt ggctcctggc
    26161 cggaagtgat gaaaacccac ttcaccagcg gcggcgagtt agacaagctg ttagcggcgg
    26221 ggcgtaactg atgtttgctg tctcctccag acgcgtgctg ccgggcttta ccttaagcct
    26281 cggcaccagt ctgctgtttg tgtgcctgat tttgctgctg ccgctctccg cgctggtgat
    26341 gcaactggcc cagatgagct gggcgcagta ctgggaggtg atcaccaacc cgcaggtggt
    26401 cgcggcctac aaagtaacgc tgctgtcggc gtttgtggca tcgattttta acggcgtttt
    26461 cggtctgctg atggcgtgga tcctaacccg ctatcgcttc ccaggccgca cgctgcttga
    26521 tgcgctgatg gatttaccct ttgcgctgcc aacggctgtc gccggtttaa cgctggcctc
    26581 gctcttttcc gtaaacggtt tttacggtga atggctggcg aagtttgata tcaaagtcac
    26641 ctatacatgg ctggggattg cggtggcgat ggcctttacc agcattccgt ttgtggtgcg
    26701 taccgtgcag ccggtgctgg aagagttagg cccggaatat gaagaagcgg cggaaacgct
    26761 tggtgcaacg cgctggcaga gtttctgcaa agtggtgctg ccggagcttt ctccggcgct
    26821 ggtggcgggc gtggcgctgt cgtttacccg tagtcttggt gaatttggcg cggtgatttt
    26881 tatcgccgga aatatcgcgt ggaagacgga agtgacgtcg ctgatgattt ttgtgcgctt
    26941 gcaggagttt gattacccgg cggcgagcgc gattgcttcg gtgatcctcg cggcatccct
    27001 gatactgctg ttctcaatta acactctgca aagtcgcttt ggtcggcgtg tggtaggtca
    27061 ttaatggcgg aagtgacaca attgaagcgt tatgacgcgc gcccgattaa ctggggcaaa
    27121 tggtttctga ttggcatcgg gatgctggta tcggcgttca tcctgctggt gccgatgatt
    27181 tatatcttcg tacaggcgtt cagcaaaggg ctgatgcctg ttttacagaa tctggccgat
    27241 ccggatatgc tgcacgccat ctggctgacg gtgatgatcg cgctgattgc cgtaccggta
    27301 aacctggtgt tcggtattct actggcgtgg ctggtgacgc gctttaactt tcccggacgc
    27361 cagttgctgc tgacgctgct ggacattccg tttgccgtgt cgccggtggt tgccggtctg
    27421 gtgtatttgc tgttctacgg ctctaacggc ccgctcggcg gttggctcga cgagcataac
    27481 ctgcaaatta tgttctcctg gccgggaatg gtgctggtca ccatcttcgt gacgtgtccg
    27541 tttgtggtgc gcgaactggt gccggtgatg ttaagccagg gcagccagga agacgaagcg
    27601 gcgattttgc ttggcgcgtc cggctggcag atgttccgtc gcgtcacatt accgaacatc
    27661 cgctgggcgc tgctttatgg cgtggtgttg accaacgccc gcgcaattgg cgagtttggc
    27721 gcggtgtcgg tggtttccgg ctcgattcgc ggcgaaaccc tgtcgctgcc gttacagatt
    27781 gaattgctgg agcaggacta caacaccgtc ggctccttta ccgctgcggc gctgttaacg
    27841 ctgatggcga ttatcaccct gtttttaaaa agtatgttgc agtggcgcct ggagaatcag
    27901 gaaaaacgcg cacagcagga ggaacatcat gagcattgag attgccaata ttaagaagtc
    27961 gtttggtcgc acccaggtgc tgaacgatat ctcactggat attccttcag gtcagatggt
    28021 cgcgttgctg gggccgtccg gttccgggaa aaccacgctg ctgcgcatta tcgccgggct
    28081 ggagcatcaa accagcgggc atattcgctt ccacggcacc gacgtcagcc gcctgcacgc
    28141 gcgcgatcgc aaggtcggtt tcgtgttcca gcattacgcg ctgttccgcc atatgacggt
    28201 attcgacaat atcgcttttg gcctgacggt gctgccgcgt cgcgatcgcc cgaatgccgc
    28261 agccatcaaa gcgaaagtga caaaattgct ggagatggtg cagcttgctc atctggcaga
    28321 tcgttatccg gcacagcttt ccggcggcca gaaacagcgc gtggcgctgg cgcgcgcgct
    28381 ggctgtagaa ccgcaaattc tgctgcttga tgaaccgttt ggcgcgctgg atgcgcaggt
    28441 gcgtaaagag ctgcgtcgct ggctgcgtca actgcatgaa gagctgaaat tcaccagcgt
    28501 gttcgtgacc cacgaccagg aagaggcgac cgaagtagcc gatcgtgtag tggtgatgag
    28561 tcaggggaat attgaacagg ctgatgcgcc gaatcaggta tggcgcgaac cagcgacccg
    28621 ttttgtactc gaatttatgg gcgaagtgaa ccgcctgcag ggaaccattc gcggcgggca
    28681 gttccatgtt ggcgcgcatc gctggccgct gggctacaca cctgcgtatc agggtccggt
    28741 ggatctcttc ctgcgcccgt gggaagtgga tatcagccgc cgtaccagtc tcgattcgcc
    28801 gctgccggta caggtactgg aagccagccc gaaaggtcac tacacccaat tagtggtgca
    28861 gccgctgggg tggtacaacg aaccgctgac ggtcgtgatg catggcgacg atgccccgca
    28921 gcgtggcgag cgtttattcg ttggtctgca acatgcgcgg ctgtataacg gcgacgagcg
    28981 tatcgaaacc cgagatgagg aacttgctct cgcacaaagc gcctgatagg ttgagtgaat
    29041 gttaaacgcc cggaggcgct tcccgcgatc cgggcttttt aatggcaagg tttgtaacct
    29101 gtagacctga taagacgcgc aagcgtcgca tcaggcaaca ccacgtatgg atagagatcg
    29161 tgagtacatt agaacaaaca ataggcaata cgcctctggt gaagttgcag cgaatggggc
    29221 cggataacgg cagtgaagtg tggttaaaac tggaaggcaa taacccggca ggttcggtga
    29281 aagatcgtgc ggcactttcg atgatcgtcg aggcggaaaa gcgcggggaa attaaaccgg
    29341 gtgatgtctt aatcgaagcc accagtggta acaccggcat tgcgctggca atgattgccg
    29401 cgctgaaagg ctatcgcatg aaattgctga tgcccgacaa catgagccag gaacgccgtg
    29461 cggcgatgcg tgcttatggt gcggaactga ttcttgtcac caaagagcag ggcatggaag
    29521 gtgcgcgcga tctggcgctg gagatggcga atcgtggcga aggaaagctg ctcgatcagt
    29581 ttaataatcc cgataaccct tacgcccatt acaccactac cgggccggaa atctggcagc
    29641 aaaccggcgg gcgcattacc cattttgtct ccagcatggg aacaaccggc actatcaccg
    29701 gcgtttcacg ctttatgcgc gaacaatcca aaccagtaac gattgttggc ctgcagccgg
    29761 aagaaggcag cagtattccc ggcattcgcc gctggcctgc ggaatatctg ccggggattt
    29821 tcaacgcttc tctggtggat gaggtgctgg atattcatca gcgcgatgca gaaaacacca
    29881 tgcgcgaact ggcagtgcgg gaaggaatat tctgtggcgt cagctccggc ggcgcggttg
    29941 ccggagcact gcgggtggca aaagctaacc ctggtgcggt ggtggtagcg atcatctgcg
    30001 atcgtggcga tcgctacctt tccaccgggg tgtttgggga agagcatttt agccaggggg
    30061 cggggattta agattattgc gcaggcaccg ttttatccgt ctgcgcaatt agcgcatgta
    30121 aaaaaggctg tgcgttctca tcgctcttac cggggatctt cacaatatac ggcttcccgg
    30181 taatcgatga cgacgaagca accttatcaa taaactgctc ggcagtgtca atgcggttac
    30241 gggtattgcc cagcttcaga cgcagatgag aaaccgcttc atcgcaggta tgttcatcac
    30301 cgttgcgcac aaatatcaaa tcctttttct gtgctaatcc ctccagcatg gcgttgatgc
    30361 gggcttcttc atgggcagtt aacttcgcgt aagcggggag tgtcattagt agtgtaatga
    30421 ccagacaaat gattttcttc attgaattac cttatccttg ttttgtgata gccgatttga
    30481 ctgccagaat caggtaaagt ttcggttgga atacgatgag gatcatcggc aaggagaaaa
    30541 aattaaatga gtagtttgtt gttgtttaac gataagagca gggcgctgca ggcggatatc
    30601 gtcgccgtgc agtcgcaggt ggtttacggc agcgtgggca acagcattgc cgtgcctgct
    30661 atcaaacaga acggtctgaa tgtttttgcc gtgccgacgg tattgctgag caatacgccg
    30721 cattatgaca ctttctacgg tggtgcgatt ccggatgaat ggtttagcgg ttatttgcgt
    30781 gcgcttcagg agcgtgatgc gctgcgccag ctgcgcgctg taaccacggg ctatatggga
    30841 acggcatcgc aaatcaaaat ccttgccgag tggctgactg cgctacgcaa agaccatcct
    30901 gacctattga tcatggtcga tccggtgatt ggcgatattg atagcggaat ttatgtcaaa
    30961 cctgaccttc ccgaggcgta tcgacaatat ttactgccgc tggcgcaggg aattaccccc
    31021 aatatctttg agctggaaat cctgaccggt aaaaattgcc gcgatctcga cagtgccatt
    31081 gctgccgcaa aaagtctgct ttcagacaca ttaaaatggg tggtgattac cagcgcctcc
    31141 ggtaatgaag aaaatcagga gatgcaggtg gtggtggtca gtgccgacag cgtgaatgtc
    31201 atttcccatt cacgggtaaa aaccgacctg aaagggactg gcgacctgtt ttgtgctcag
    31261 cttatcagtg gtttgctgaa agggaaggcg ttaaacgatg cggtgcaccg tgcggggttg
    31321 cgcgtactgg aagtgatgcg ctacacccag cagcatgaga gcgatgaatt gattttgccg
    31381 ccgctggcgg aagcataaaa aatggcgccg atgggcgcca tttttcactg cggcaagaat
    31441 tacttcttga tgcggataac cggggtttca cccacggtta cgctaccgga cagtttgatc
    31501 agttctttga tttcgtccat gttggagata acaaccggag tcagggtaga cttggctttc
    31561 tcttccagta gcggcagatc aaattcaatg acagtatcgc caactttcac gcgctgacct
    31621 tcttcagcaa tacgcttgaa gccttcgcct ttcagttcaa cggtgtcgat accgaagtgg
    31681 acgaacagtt caacgccgct atcagattcg atagagaacg cgtgattggt ttcaaagatt
    31741 ttaccaatgg taccgtctac aggcgcgacc attttgttac ccgttggttt gatagcaata
    31801 ccatcaccaa cgattttttc cgcaaaaacg acatccggca cgtcttcgat attgacgatc
    31861 tcgccagaga gcggagcaat gatctcaata gttccggtat ccttcttgtc gtcggaaacc
    31921 agagatttca gtttatcgaa caaacccatg atcttctcct aagcagtaaa ttgggccgca
    31981 tctcgtggat tagcagattg ttttttcttc aatgaacttg ttaaccagcg tcatcaactc
    32041 gtccgttgtc ggttgagcaa gagcctgctc tgctaacacc ttcgcatctt cgaagttcgt
    32101 gttacggata atcttcttaa tgcgcgggat agaaatggcg ctcatagaga attcgtccag
    32161 acccatcccc agcaacagaa gtgtagcacg ttcatcgcca gcaagctcac cacacatgcc
    32221 agtccatttg ccttcagcat gagaagcatc aataacttgc ttgatcaagt tcagcacgga
    32281 cggtgacatt ggttggtaaa ggtgtgaaat catatcatta ccacggtcaa ctgccagagt
    32341 gtactgcgtt aaatcattgg tgccgatact aaagaaatca acttctttgg ctaaatgacg
    32401 tgcaattgtt gcggcagccg gtgtttccac cattacgccg atttcaattg actcgtcaaa
    32461 cgctttacct tcgtcgcgca gttcctgttt gtagatttcg atctctttgc gcagtgcacg
    32521 cacttcttca acagagatga tcatcgggaa cataatgcgc aatttaccga aagccgaggc
    32581 acgcaggata gcgcggagct gatcgcgcag gatctcttta cgatccatcg cgatacggat
    32641 agcgcgccag ccgaggaacg ggttctcttc tttcgggaag ttcatgtatg gcagctcttt
    32701 gtcgccgccg atgtccatgg tacgaacgat aaccgcttgc gagccacacg cttcagccac
    32761 tgctttgtaa gcagcaaact gttcttcttc agtgggcagc gcgtcgcggt ccatgaacag
    32821 gaactcagta cgatacagac caacgccttc agcgccgtta cgctctgcac cttcaacgtc
    32881 acgaaccgta ccaatgttag cgcatacttc tacctggtga ccgtccagcg taatagctgg
    32941 cagatctttc agtttagcaa gctctgcttt ttcagaagcc acttgctcct gaacagcgcg
    33001 cattttatca ataacttcgt tggttggatt gacgtaaacc tgattattta cggcatccag
    33061 aatcagatag tcgtcatttt tcacctgaga ggtgacgcta ccggtaccca cgatagcagg
    33121 cagttccaga gaacgcgcca tgatagaggt gtgggaagta cggccacccg cgtcggtgat
    33181 gaaacccagc accttcttca ggttcagctg tgcggtttcg gacggcgtca ggtcagcggc
    33241 aaccagaatg acttcatcct gaatggcgct caggtcgata atcttcaggc ccaggatgtt
    33301 gcgcagcagg cgcttaccga tatcacgtac gtcagccgca cgttctttca ggtattcatc
    33361 atccagctct tccagggcag aagcctgacc ttcgataact tcatgagcag ctgcgtcagc
    33421 tgtcatgtgc ttatctttaa tcagggctat gatttcctgc tccagctcct catcttcgag
    33481 cagcataata tgcccttcaa agatggcttc tttttcttca ccgaacgttt caccagcttt
    33541 cgttttgatc gtttccagct gggctgatgc cttggcacga ccgctcagaa aacgttcaac
    33601 ttcctgatca acctggtcgg cagaaatttt tttccggtca atgacaattt cgtcttcttt
    33661 cagaagcaga gctttaccga aagcgatacc cggggatgct aaaatgcctg aaatcataac
    33721 cctaccttac ttgtgactga tttttaaaag aacccgggaa attactcgag ttccgccatc
    33781 agtttaacca gatgttcaac cgctttctgc tcgtcttcgc cttctgcgga gatagtcaca
    33841 acggtacctt gagtcaggcc cagagtctgc agtttaaaca ggcttttcgc gctggcgctt
    33901 ttgccgttgg aagtcacagt aatttcagaa gtgaagccct tagcttcttt tacaaactgg
    33961 gcagcagggc gggtgtgcag accgttcgga gcggtaatgg taacttcttg ctggaacatt
    34021 gtatttcccc aacttatagg tttagtgttg tggaactaaa gtctagcctg gcggttcgac
    34081 tttagcctgt attgttagcg ccagtttcta acagacgcga cgcacgaaga gcgcaataca
    34141 gtccattgct ggcgggtgtt gaccgctgac gtttcgtacg tcattaaaca ttatgccgct
    34201 aaaggaagaa ttgaaccaaa tcataaaatc gattcagctt tggcttttcc tgtaacgatt
    34261 aatttcgcgc atcaaaataa ttagtctggt taaataccag ttccgacagg ctgaatcaat
    34321 gccaggaggg gcgaaagttt gaagcaggcc acaaaaaagc acctaaaaag gtgctttttt
    34381 acgcattttt aacaagctgg cattactgtt gcagttcttt ctcagtgaag agatcggcaa
    34441 acaatgcggt gcttaaataa cgctcacccg atgacggtag aataaccaca atattcttgt
    34501 tggtaaagct ttcatcttct tgtagtttca acgccgcagc aacagctgct ccagaagaga
    34561 tacctgcaag aataccttct tcttccatca gacgacgtgc ggtagaaatc gcttcttcat
    34621 tggtgatgcc aatgacttta tcgaccagct tgagatcgag gttagctggg ataaaaccag
    34681 cgccaatacc ctgaatttta tgcgggccag gtttaatctc ttcaccagcc agtgcctggg
    34741 cgataactgg agaatcggtt ggctcaacgg cgacagagat aagatcggtc ttgcctttgg
    34801 tgcctttaat gtagcggctg acgccagtca gcgtaccgcc agtcccaacg ccagcaataa
    34861 atacatcaac ctgaccgtcg gtatcttccc agatctccgg accggtggtt ttttcgtgaa
    34921 tttcagggtt tgccggattg ctgaattgtt gcagcagcag gtatttctct ggattgctgg
    34981 cgacaatttc ttctgctttt tggattgcgc ctttcatgcc tttagcacct tccgtcagca
    35041 ccaggtttgc acctaacgct ttcagcagct tgcggcgttc aatactcatg gtttctggca
    35101 tggtcagggt gagtttgtaa ccgcgagcgg cagctacata ggccagtgca atcccggtat
    35161 taccgctggt cggttcaacc agttcaacgc ctggtttcag cacgccgcgc ttttcggcat
    35221 cccaaatcat gttggcaccg atacggcact taacgctgaa gctggggtta cgagattcca
    35281 ccttcgccag aatgcgtccg ttaccgatgc gattcaggcg aaccagcggc gtgtgaccga
    35341 tagtcagcga gttatcttca aaaatcttac tcatggcctg tccttaactg tatgaaattg
    35401 ggatacaaca ggtagcatac ccgctcagag aatatgcgga agtaaggatt tagcatatct
    35461 atatacagaa gggaaataat gacatgcaag atggaataag gggcggcata agccacccct
    35521 gtttcacaca aacggtttac aaaataaccg gtagattgtt accgccacat cgcgtgttta
    35581 tcgcgatagc aatcgaccca catcgccgtc gcgccacaaa cggcaacggg catgatgaac
    35641 agattaagca gcgggatcat cgtaaacagg ctggttaaag caccaaactg catattggtg
    35701 attttgcgtg tgcgcagggc ggtgcgcatc tctttaaacg gcactttgtg gttatcgaag
    35761 gggtaatcgc aatactggat ggctaacatc caggcgctaa acaggaacca cagtaccggc
    35821 gcgacggttt gcccaatacc agggattaag taaagaatta gcaatacaat tgcgcgcggc
    35881 agataccagg caaatttttg ccattcgcgt ttcatgattc gcggcacatc tttcatgata
    35941 ccgaaaatcc cggtatctgg cggtgtagcg ccagtcaatc gtgcttccag ttgttcagcc
    36001 aataaaccgt taaacggagc ggcaatccag ttagcaatcg tggagaagaa atagccaaac
    36061 actaacagca cagagatgac agccagaggc cacaacagat aactcagcca ttgtagccag
    36121 tccggaacgt aactcatgag agtcgggatc cagacatcga gctgtgtaaa gagccaccag
    36181 aatgcgcccc ccatcaacaa aatattgacc agcagcggta aaataacgaa acgccgaatc
    36241 ccaggttgcg agacgagctt ccagccttgc gcaaaatagt aaaaaccgct gcgtggggca
    36301 gatgtgaatg atgaaaccat aatcaggatg agctcctttt gaccaatccc aggaaaattc
    36361 tgcgtatttt accgggtaat tgcgcaatgg acagttagga tatgttcgaa aaaacagcaa
    36421 aaagcacgat ttcatctatc tttgtgctgt gaaagttaat agtgcacttg cacttgaggt
    36481 aatcggcaaa tactcttagt gagtaaatgt ttgccgtggt ggcaaggtgt tagaacaaca
    36541 gagaatataa tgatgcagga tttgcgtctg atattaatca ttgttggcgc gatcgccata
    36601 atcgctttac tggtacatgg tttctggacc agccgtaaag aacgatcttc tatgttccgc
    36661 gatcggccat taaaacgaat gaagtcaaaa cgtgacgacg attcttatga cgaggatgtc
    36721 gaagatgatg agggcgttgg tgaggttcgt gttcaccgcg tgaatcatgc cccggctaac
    36781 gctcaggagc atgaggctgc tcgtccgtcg ccgcaacacc agtaccaacc gccttatgcg
    36841 tctgcgcagc cgcgtcaacc ggtccagcag ccgcctgaag cgcaggtacc gccgcaacat
    36901 gctccgcgtc cagcgcagcc ggtgcagcag cctgcctatc agccgcagcc tgaacagccg
    36961 ttgcagcagc cagtttcgcc acaggtcgcg ccagcgccgc agcctgtgca ttcagcaccg
    37021 caaccggcac aacaggcttt ccagcctgca gaacccgtag cggcaccaca gcctgagcct
    37081 gtagcggaac cggctccagt tatggataaa ccgaagcgca aagaagcggt gattatcatg
    37141 aacgtcgcgg cgcatcacgg tagcgagcta aacggtgaac tgcttcttaa cagcattcaa
    37201 caagcgggct tcatttttgg cgatatgaat atttaccatc gtcatcttag cccggatggc
    37261 agcggcccgg cgttattcag cctggcgaat atggtgaaac cgggaacctt tgatcctgaa
    37321 atgaaggatt tcactactcc gggtgtcacc atctttatgc aggtaccgtc ttacggtgac
    37381 gagctgcaga acttcaagct gatgctgcaa tctgcgcagc atattgccga tgaagtgggc
    37441 ggtgtcgtgc ttgacgatca gcgccgtatg atgactccgc agaaattgcg cgagtaccag
    37501 gacatcatcc gcgaagtcaa agacgccaac gcctgataca cttaaggcaa attaactcct
    37561 cttcgaaccc ccgcttgtcg ggggttttta gcattgatgg tgcgatatgg aatcaatcga
    37621 acaacaactg acagaactgc gaacgacgct tcgccatcat gaatatcttt atcatgtgat
    37681 ggatgcgccg gaaattcccg acgctgaata cgacaggctg atgcgcgaac tgcgcgagct
    37741 ggaaaccaaa catccagaac tgattacgcc tgattcgcct acccaacgtg taggcgctgc
    37801 gccgctggcg gctttcagcc agatacgcca tgaagtacca atgctgtcac tggataacgt
    37861 ttttgatgaa gaaagctttc ttgctttcaa caaacgtgtg caggaccgtc tgaaaaacaa
    37921 cgagaaagtc acctggtgct gtgagctgaa gctggatggc cttgccgtca gtattctgta
    37981 tgaaaatggc gttttagtca gtgccgcgac ccgtggcgat ggcaccaccg gggaagatat
    38041 cacgtctaat gtgcgtacta ttcgcgctat tccgctgaag ctgcacggag agaatatccc
    38101 ggcgcgtctg gaagtgcgtg gtgaagtgtt cctgccgcag gcggggttcg aaaagattaa
    38161 cgaagatgcg cgacgcacgg gcgggaaagt ttttgctaac ccacgtaatg cggcagctgg
    38221 ttcactgcgt cagcttgatc cgcgtattac agcgaagcga ccgctcactt ttttctgcta
    38281 tggcgttggt gttctggaag gtggcgagct gccggatact catcttggcc gtttactgca
    38341 atttaaaaag tgggggttgc cggtcagcga tcgggtaacg ctttgtgaat cggcggaaga
    38401 agtgctggcg ttctatcaca aagtggaaga agaccgcccg acgctgggct ttgatatcga
    38461 cggcgtggtg attaaggtca actcactgga acagcaggag cagcttggct ttgtcgcgcg
    38521 tgccccgcgc tgggcggtag cgtttaaatt cccggcgcag gagcagatga cctttgtgcg
    38581 tgacgtcgag tttcaggttg ggcgtactgg cgcgattacg cctgttgcgc gtctggaacc
    38641 tgtccatgtt gctggcgtgc tggtgagtaa cgcaacctta cacaatgcgg atgaaatcga
    38701 acgtcttggt ttacgcattg gcgataaagt ggtgattcgc cgcgctggcg acgtgatccc
    38761 gcaggtggtt aacgtcgtgc tttctgaacg cccggaagat acccgtgagg ttgtattccc
    38821 gacgcattgt ccggtatgtg gttctgacgt tgagcgtgtg gaaggtgaag cggttgcccg
    38881 ctgtaccggt ggcctgattt gcggagcgca gcgtaaagag tcgctgaaac actttgtttc
    38941 ccgccgtgcg atggatgttg acggaatggg cgacaaaatc atcgatcagc tggttgaaaa
    39001 agaatatgtc catactccgg cagatctgtt caaactcacc gcaggcaaac tgaccggact
    39061 ggagcgtatg gggccaaaat cggcacaaaa cgtggttaac gcgctggaaa aagcgaaaga
    39121 aaccaccttt gctcgcttcc tctatgcact tggcatccgt gaagtcggcg aggccaccgc
    39181 agcaggtctg gcggcatatt tcggcacgct ggaagcgctg gaagccgctt cgattgaaga
    39241 gctgcaaaag gtgcctgatg ttggcattgt cgttgcatcc cacgttcaca acttctttgc
    39301 cgaagaaagc aaccgcaatg tcatcagcga gctgttggcg gaaggtgttc actggcctgc
    39361 gccgatcgtt atcaacgcgg aagagattga cagcccgttt gctggtaaaa ccgtggtgct
    39421 tacgggcagc ttaagccaga tgtcgcgtga tgacgctaaa gctcgactgg ttgaactggg
    39481 cgcgaaagtc gcgggcagcg tgtcgaagaa aaccgatctg gtgattgccg gagaagcggc
    39541 aggttctaaa ctggcgaaag cgcaggaact gggcattgaa gtcatcgacg aagcggaaat
    39601 gctgcgtttg ctgggtagct gagatggaaa aagagcagct gattgaaata gccaatacga
    39661 taatgccgtt tggcaaatat aaagggcgtc gcttaatcga cctgccggag gagtatctgc
    39721 tgtggtttgc ccgcaaagat gaattcccgg cagggaagct cggtgagcta atgcaaatca
    39781 cgctgctgat taaaaccgag gggctgacgc aactggtcca gcccctgaag cgtccgcttt
    39841 aagctttatc ggcgctgctt tcctgctgtg cctgtaactg ttcggtctgg cgtttgtatc
    39901 gacgcgccag caccgcacag accatcaatt ggatctgatg gaaaatcatc aggggcagca
    39961 ccatcatacc gatcaccgat gtggggaaca gaatgtttgc catcgggatg ccatttgcca
    40021 gactcttttt cgaaccacaa aagacgatag taatttcatc tgccttattg aagcccagtc
    40081 ggcgtgccat aaagacgtta actacaatca cgatagccag aagaacgcag ctgaccacca
    40141 cgataaacag caatgatccc cagccaactt tatgccagat accattaacg acggcttcgc
    40201 tgaacgctgt ataaaccacc aacagaatgg acgtctggtc agttttcgca atccattttt
    40261 tattgcgcga cacccagtca ccaatccacg gccgggaaag atgccccaac acaaacggca
    40321 gcagcagttg cagcataatt ttaccgacct gctcaaggct gccccctgca ccgtgaacat
    40381 tcatcaccag accaaccagc aatggtgaaa ggaaaatccc cagcaggctg gatgctgacg
    40441 cagaacaaac cgccgccgcg acgttaccgc ccgccattga cgtgaaggcg attgcagact
    40501 gcacggtagc cgggagaatg cacaagtaga gaaaaccgga gtagagcatc gggtcgacat
    40561 ttaccggttt ccaccaggca aacagtacac ccagaatcgg aaacagcacg aaggtgctgc
    40621 aaattaccca caaatgcagt cgccagtgac caccgccagc aataatcgcc tcacgcgaca
    40681 acttcgcgcc gtgcataaag aacagcaggg caatcgctgc ggtggtcaga ttttcaaaga
    40741 aggggacgaa atcgcctctg gccggaaaga aagaggccag caacaccacc gtgatcaggg
    40801 ttaaggtgaa aggatcgagg atacgaaaaa gtttcataaa gactccagat gatcgatggt
    40861 tctattgtgc ttcttccagt ttgagaaata aaattgattt attgcatcca ttcatgaaga
    40921 aaacagatga attattcttt aaaacaatta aaggttttcg tcacagtagc gcaggagaaa
    40981 agttttagtc gtgcaggaga gcgtatcggc ctgagccagt cggcagtgag tcacagtgtg
    41041 aaggaactgg aaaatcatac tggtgttcgc ctgctggaca gaaccacgcg tgaagtggtg
    41101 cttacagatg cagggcagca gctggctttg cgtcttgagc gactgctgga tgaactgaac
    41161 agcacgttgc gcgataccgg gcgtatgggg caacaactga gcggaaaagt tcgggtcgct
    41221 gccagccaga ccatttccgc gcatcttatt ccgcaatgca ttgctgaaag ccatcgccgc
    41281 tatccagata ttcagtttgt cctgcacgat cgtccgcagc agtgggtgat ggaaagtatt
    41341 cgccagggag atgtcgactt tggcatcgtc atcgatccag gcccagtggg cgatctgcaa
    41401 tgtgaagcga ttctttccga gcctttcttt ctgctttgcc atcgcgatag cgctttggcc
    41461 gtcgaagatt atgtaccctg gcaggcatta cagggagcga aattggtgtt gcaggattac
    41521 gcgtcaggca gccgaccgct gattgacgca gcgctggcgc gcaatggtat tcaggcgaat
    41581 attgtgcagg agattggaca tccggcgacg ctgttcccga tggtagccgc aggcatcggc
    41641 atcagtattc ttcccgcact ggcgctacca ttgcctgaag gtagcccact ggtggttaag
    41701 cgcattacgc cggtggttga acgccagttg atgctggtgc gcaggaagaa ccgttcactt
    41761 tccaccgccg ctgaagcgtt atgggacgtg gtacgagacc agggcaatgc gctaatggct
    41821 gggcgggaag gggatccgtt gtatcagata tagatatcga tatgagtcgt tttttcaaca
    41881 ggatttagaa gggatacatt cagagacaaa cactgctctt tctcctgcgc cttttctgcc
    41941 tgctgtttct gcaactgcgc cagttgtgtt tccagtaggg tgatttgctg ttgaatcaat
    42001 tcggcctgtt tttgcttgtc ttcagcactg ccgctagtgt ctacaatctc tttaatttgt
    42061 tgagtgagtt tgattatttg ctgacttatc cgcgcgattt gtgaagcaac atcgctacct
    42121 tgtgatgccc tgttactact ctgtgtttgt atggaaggaa ttgaagttga agagatagtg
    42181 gccattgttc agtcctcgtc actgtacgag gtaatatcgg tagaagaaca agcaacttga
    42241 gaagaaaaat caatcttaca aatcgcagat agcaaaaaag cgcctttagg gcgctttttt
    42301 tacattggtg ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag tcaactgctc
    42361 taccaactga gctaacgacc cgaagtggtg ggtgatgacg ggatcgaacc gccgaccccc
    42421 tccttgtaag ggaggtgctc tcccagctga gctaatcacc cacttcggta cttcacattt
    42481 ttacaagaaa tttagctggc gagaaagtgg tgggtgatga cgggatcgaa ccgccgaccc
    42541 cctccttgta agggaggtgc tctcccagct gagctaatca cccacttctc aatttcttgc
    42601 tacacggcgg agactacata aagtagttgg tgggtgatga cgggatcgaa ccgccgaccc
    42661 cctccttgta agggaggtgc tctcccagct gagctaatca cccccgctgt gtggagtcgc
    42721 attataggga gagttcaaaa tgagtcaacg cattttctaa agaaattgtt cgttcgtcgt
    42781 aaatttaagc aagatgatcg caaaacagac cgtgttgcgc aatttgtcaa cgaaaacaat
    42841 aatgcgtaag gttgcaaccc gaactacatt gaggaatcag gcgggagtga tagaatatcg
    42901 cccactcaat ttttccagga tttgccggtt gtcggcatct ttctaaacgt aaggccattt
    42961 catgaaaatc aaaactcgct tcgcgccaag cccaacaggc tatctgcacg ttggcggcgc
    43021 gcgtactgct ctttactcct ggctttttgc acgtaaccac ggcggtgagt tcgtgctgcg
    43081 tattgaagac accgatcttg agcgttccac gccggaagct atcgaagcca ttatggatgg
    43141 catgaactgg ctgagcctgg agtgggatga aggtccgtac taccagacca aacgttttga
    43201 tcgctacaat gcggtgatcg atcagatgct ggaagagggc actgcttata aatgctattg
    43261 ctctaaagag cgcctggaag cgctgcgcga agagcaaatg gcgaaaggtg agaagccgcg
    43321 ttatgacggt cgctgccgcc acagccatga gcatcatgct gatgatgaac cgtgtgtcgt
    43381 gcgttttgct aatccgcagg aaggttctgt tgtttttgac gatcagatcc gtggtccgat
    43441 cgagttcagc aaccaggagc tggatgatct gattatccgc cgtaccgatg gttccccaac
    43501 ctataacttc tgtgtggttg tcgatgactg ggatatggaa atcacccacg ttatccgtgg
    43561 cgaagaccat atcaacaaca cgccgcgcca gatcaacatt cttaaggccc tgaaagcgcc
    43621 ggtgccggtt tacgcgcacg tttctatgat caatggcgat gacggtaaaa aactgtccaa
    43681 acgtcacggg gcagtcagcg taatgcagta tcgtgatgac ggttatttgc cagaagcact
    43741 gctgaactat ctggtgcgtc tgggctggtc ccacggcgat caggaaatct tcactcgtga
    43801 agagatgatc aaatacttca ctttgaatgc cgtcagcaaa tctgccagtg cgttcaacac
    43861 cgacaagctg ctgtggctga accatcacta cattaacgcg ctgccgccgg agtatgttgc
    43921 tactcactta cagtggcaca ttgagcagga aaatatcgat acccgtaacg gcccgcagct
    43981 ggctgatctg gtgaaactgc tgggcgaacg ctgcaagacg ctgaaagaga tggcacagag
    44041 ctgccgttat ttctacgaag attttgctga gttcgatgcc gatgcggcga aaaaacatct
    44101 gcgtccggta gcgcgtcagc cgctggaagt ggttcgtgac aaactggccg cgattactga
    44161 ctggaccgct gaaaacgttc atcacgctat tcaggcgacg gcggatgagc tggaagtggg
    44221 tatgggtaaa gttggtatgc cgctgcgtgt cgccgtaacc ggtgcggggc agtctccagc
    44281 actggatgtt accgttcacg caattggtaa gacccgcagt atcgagcgta tcaacaaagc
    44341 gctggatttt attgctgaac gcgaaaatca gcagtaatta gcgtgtaaaa agataaacgg
    44401 caggagataa tatcctgccg ttttttattt atgctgattc gtcaatatcg agacgggcga
    44461 ggaaattgcg aattccttca cgtgacaaaa attgcgcgac tttttcctgc tcaatgtctg
    44521 tcatattatc tatcgcgttg atgatttgtc gatacgccgg ggcgcgtgct ccaggagcgt
    44581 attcagcaat tctctcttca gcttccatca acattggtgt gttatggaag gctggcgtgt
    44641 tctgaataaa ctcgcaaact tgcgtatcga ccagaataag tctggcacga ccgcctttca
    44701 cgccgggaaa tttttccgtt ttccagcctt tttctctgat ccaacggtta acagtttgtt
    44761 tagccacacc gaggcattca gccagttctt cggtggtcat tttgttgcgt aatcttttca
    44821 tattatttgc tatcgcgaat ccctaagcgt tgtaacaatc cgataatccc ttcccgcaga
    44881 agcaaggatg taaactgttt ttgttcaact ggcgtcatct ctttcgccag tgtcaccagc
    44941 aatacttcaa gcggcgcatc accagaaagg gcaggcgctt ctccctgacc ttctggacgt
    45001 tcagcattgc gaatatattc acgaacctgt tcattgacgt gaaccagtcg ggctttgcca
    45061 ccctggatgc caggttttgg tgacgttgtc cagccttcct tgcgtaccca tttattaatg
    45121 gtctggcggc tatagccggt cagtctggca agttcatctg gtgtcatcct ctccttgaac
    45181 ataacaattc cctgaatgta tgtgggtcta ttagtagttc attttataac accactttac
    45241 aggaaaccgt gacatgaata actactgaat gcgagcggtt ttccaaaatt gttcatttgc
    45301 ctgatttttc agcgattgaa attacacgtt gcaatttgcc gttgacacat tcgggcggaa
    45361 ttcatatgat gccgcccgtc aactcgacaa gctttacgtg acggggctat agctcagctg
    45421 ggagagcgct tgcatggcat gcaagaggtc agcggttcga tcccgcttag ctccaccaaa
    45481 ttttgcaccc agcaaatttg gtacgtaaac gcatcgtggg gctatagctc agctgggaga
    45541 gcgcttgcat ggcatgcaag aggtcagcgg ttcgatcccg cttagctcca ccaaatttcc
    45601 aaccctcgct gcaaagcggg ggttttttgt ctctgctttt tgccgctttt gtaatacagt
    45661 ctacgtccgg gttagcgccg cctggtgaaa gcatcattgg atgaaaaatc ggcaacaggc
    45721 tggcccctgt ttgcttcgcg atgcgaataa acttattatt tgtgtgcctg aaaaccccga
    45781 tcagtgagag tagtgtactc atgtttgtgg agcataacct gataaaaaat atcaagatat
    45841 tcacactagc gtttacgctc accgtggtac ttattcagct atcccgtttt atttcgccac
    45901 ttgccattat ccattccagt tatatctttc tggcgtggat gccactgtgc gtaatgctgt
    45961 caatcttgtt tatctttggc tggcgcggtg tcgttcccgt tttatgcggg atgttttgca
    46021 ccaatctgtg gaactttcat ctctcttttt tacagactgc ggtcatgctc ggtagccaga
    46081 cgtttgtcgt gttgtgtgcc tgcgcaatat tacgctggca gctggggacg cgttggcgtt
    46141 atggattgac cagccgatat gtctggcaac gtctgttctg gcttggtttg gtgacgccga
    46201 tcggcatcaa atgcagcatg tatcttgtgg ggagcttctt tgatttcccg ctaaagatat
    46261 ccaccttttt cggcgatgcg gatgccattt tcacggtcgt tgatttgcta agccttttca
    46321 ccgctgtgct gatttacaac atgcttttct actatctcac tcgcatgatt gtaagtcccc
    46381 actttgcgca gatattgtgg cgcagggata tcgcgccgtc gttgggcaaa gagaaacgcg
    46441 catttacctt aagctggctg gcagcgttaa gcgtgcttct acttctgttg tgcacacctt
    46501 atgaaaacga ctttattgcc ggttacctgg tacccgtttt cttcatcatc tttaccctcg
    46561 gggtcggtaa gcttcgctat ccgtttttaa atctcacctg ggctgtttca acgctttgcc
    46621 ttctgaatta caaccagaac tttttgcaag gggtggaaac cgaatattcg ctggcattta
    46681 ttcttgcggt gctgatttcc tttagcgttt gcctgctcta tatggtgcgc atttatcatc
    46741 gcagtgaatg gcttaatcgc cgctggcatt tgcaggcgct gacagatccg ttaacgctcc
    46801 tacccaactt tcgtgcgttg gaacaagcgc cggaacaaga ggcgggcaag agtttttgct
    46861 gcctgcgcat tgataatctt gagtttatga gtcgtcatta cggcttaatg atgcgcgttc
    46921 actgtatccg ctcaatttgc cgtacgctgc tgccgttgat gcaggaaaac gaaaagttgt
    46981 atcaattgcc gggtagtgaa ctgctgttag tgctgagcgg gccggaaacg gaagggcgac
    47041 tccagcatat gattaacatc ctgaatagtc ggcaaattca ctggaacaat accgggctgg
    47101 atatgggcta tggtgctgcc tgggggcgtt ttgatggaaa tcaggaaacc ctgcaaccct
    47161 tgttggggca gttaagctgg ctggcggagc aatcctgcgc acatcatcat gtgctggcgc
    47221 tggatagcag agaggagatg gtttccgggc agaccactaa acaggtgcta ttgctgaata
    47281 ccattcgcac ggcgttagat cagggtgatt tgctgctcta cgcccagcca attcgcaaca
    47341 aagagggtga aggttatgat gagatcctcg cgcgactgaa atatgacggc ggcattatga
    47401 ccccggataa gtttctgccc cttattgctc agtttaacct tagcgcgcgt tttgatttgc
    47461 aagtgctgga atccttgttg aagtggctgg caacacaccc ttgcgacaaa aaaggaccgc
    47521 gcttttcagt caatttaatg ccgctcacgc tgctgcaaaa gaatattgcc gggcggatta
    47581 ttcgtctgtt taagcgttat cacatctccc cgcaggcggt cattcttgag atcaccgagg
    47641 agcaggcgtt ttctaacgca gaaagcagca tgtacaacat cgagcagctg cataagtttg
    47701 gtttccggat tgcgattgat gactttggca ccggatatgc caactacgaa cggttaaagc
    47761 gtttgcaggc tgatatcatc aaaattgatg gcgtctttgt gaaagatatt gtcacgaaca
    47821 cgctggatgc gatgattgtg agatcaatta ccgatctggc gaaagcgaag tcattgagtg
    47881 tggtcgcgga gtttgtcgag acgcaacagc agcaggcgct attgcataag ctcggggtgc
    47941 aatatctgca agggtatttg attggtcgcc cgcagccatt agctgattaa caggcgtaaa
    48001 aaaaccgggg aattatcccc ggctttttta tgtatggtct tacagcacca gtgctgcgat
    48061 tgacgcagac agcacactca ccagggtaga gccgtaaacc agcttcagac cgaagcgaga
    48121 aaccacgtta ccttgctctt cattcaggcc tttaactgca cctgcgataa tcccgattga
    48181 agagaagtta gcgaaggaaa ccaggaacac agagatgatg ccttcagcac gcggagagag
    48241 cgtggaagca attttctgca gatccatcat cgcaacgaac tcgttggaaa ccagtttggt
    48301 cgccatgata ctgcccactt gcagtgcttc actggaagga acacccatca cccatgcaat
    48361 cggatagaag atgtagccca ggatgccctg gaaggagatg ctgtagccaa accagccagt
    48421 aacggtggca aacagtgcgt tcagcgcggc gatcagggcg ataaagccga tcagcatcgc
    48481 ggcaacgata atggcaactt tgaaacctgc cagaatgtat tcacccagca tttcgaagaa
    48541 gctctgacct tcgtgcaggt tggacatctg gatgttttct tcactggcat caacacggta
    48601 aggattgatc agcgacagca cgataaaggt gctgaacatg ttcagtacca gcgcagcaac
    48661 gacgtatttc ggttccagca tggtcatgta tgcaccaacg atggacatcg acacggtgga
    48721 cattgccgtg gcagccatgg tgtacatacg attacgggag attttgccga ggatatcttt
    48781 ataggcaata aagttttcag actgacccag aatcagggag ctgacggcgt taaaggattc
    48841 cagtttgccc atgccgttga ctttggagag caggaaacca attgcgcgga tgatcaccgg
    48901 caacacgcga atgtgctgga gaataccgat cagtgcagag ataaagacga ttgggcacag
    48961 cactttcagg aagaagaatg ccaggccttg atcattcatg ctaccaaaga cgaagttagt
    49021 cccttcgttg gcaaatccga gcagtttttc gaacatttcg gagaagcctt tcacgaagcc
    49081 taaaccaacg tcggagttca ggaagaacca cgccagtaac acttcgataa caagcagttg
    49141 aataacataa cggatacgaa tttttttgcg gtcgctgctt accagcagtg cgagaatcgc
    49201 aacaacggca agtgccagta caaaatgaag gacgcggtcc atatttgctc caaatatgag
    49261 gcaggttaaa tttccgtgca cattctatgt aacatggtta aggaaaacga gatctgacac
    49321 acactataat gacgtcctgt gacaagcttc aaaaatagtg atctgtcata catttctgct
    49381 atgttgtgta tggaagctga aagttatgta aatgtgctaa cattaaacat aaccataaca
    49441 ttcaagtcat caatcttttc gtagtcataa tcctggtcta tcaaagaaat caccacaacc
    49501 cattcaaatg aatttgataa tcattctcgt ttggcatagc atgaaacata gcaaaggcta
    49561 tgttttagag gcacaagatg acgaactatc gcgttgagag tagcagcgga cgggcggcgc
    49621 gcaagatgag gctcgcatta atgggacctg cgttcattgc ggcgattggt tatatcgatc
    49681 ccggtaactt tgcgaccaat attcaggcgg gtgccagctt cggctatcag ctactgtggg
    49741 ttgtcgtttg ggccaacctg atggcgatgc tgattcagat cctctctgcc aaactaggga
    49801 ttgccaccgg taaaaatctg gcggagcaga ttcgtgatca ctatccgcgt cccgtagtgt
    49861 ggttctattg ggttcaggca gaaattattg cgatggcaac cgacctggcg gaatttattg
    49921 gtgcggcgat cggttttaaa ctcattcttg gtgtttcgtt gttgcagggc gcggtgctga
    49981 cggggatcgc gactttcctg attttaatgc tgcaacgtcg cgggcaaaaa ccgctggaga
    50041 aagtgattgg cgggttactg ttgtttgttg ccgcggctta cattgtcgag ttgattttct
    50101 cccagcctaa cctggcgcag ctgggtaaag gaatggtgat cccgagttta cctacttcgg
    50161 aggcggtctt cctggcagca ggcgtgttag gggcgacgat tatgccgcat gtgatttatt
    50221 tgcactcttc gctcactcag catttacatg gcggttcgcg tcaacaacgt tattccgcca
    50281 ccaaatggga tgtggctatc gccatgacga ttgccggttt tgtcaatctg gcgatgatgg
    50341 ctacagctgc ggcggcgttc cacttttccg gtcatactgg tgttgccgat cttgatgagg
    50401 catatctgac gctgcaaccg ttgttaagcc atgctgcggc aacggtcttt ggattaagcc
    50461 tggttgctgc cggactgtcc tcaacggtgg tggggacact ggcggggcag gtggtgatgc
    50521 aggggttcat tcgcttccat atcccgctgt gggtgcgtcg tacagtcacc atgttgccgt
    50581 catttattgt cattctgatg ggattagatc cgacacggat tctggttatg agtcaggtgc
    50641 tgttaagttt tggtatcgcc ctggcgctgg ttccactgct gattttcacc agtgacagca
    50701 agttgatggg cgatctggtg aacagcaaac gcgtaaaaca gacaggctgg gtgattgtag
    50761 tgctggtagt ggcgctgaat atctggttgc tggtaggaac ggcgctggga ttgtagttga
    50821 atgagcgtcg catctggcac tattggcgga tgcggcgtaa acgccttatc cgccctacgc
    50881 gttaaagagc cggtttgtag gcctgataag acgcgctagc gtcgcatcag gcattgtgct
    50941 ccattggcga atgtgtttaa tggcggtgtc catgtccgcg gccttttccg cgatgatcgt
    51001 cacgatcgcg ccagccttca cgatagccac gctcatacgc tttacgctta tcccagccac
    51061 gatgatagcc attatcatga cgccaccagc ggtttttacg ccattcataa ttgcgatgcc
    51121 agtagtcacg gtcgcgccag tgaccaccgt cccagtaatt accgtaatga tcgcgatcgc
    51181 caatttgtaa tttgattgat ggcagtaggg tgatttcacc tgcgtttgct acaagcgggg
    51241 taaatgccat cagggcggcc gccagaaaca gtgacctgaa cattgttatt ctccttcacg
    51301 atcgaagccg tcagcggcct gttaacgcaa tattacgggg aggtaaagcc ccgtatcatc
    51361 gccataactc ttaaatcata aaggagagca ttttttgcta aacgttatcg tggggtggct
    51421 gttgcttcgg acgattgacc atcatcatcg gcttacctgc taataacagc catgccggaa
    51481 gcatgacgat acagagcagc ggtagcaatg tggtattggg tacaacgact gccgccataa
    51541 aaagacttaa ccagccatcg cgtgttacca ccagcacgat gccgagaata gcgcaagaaa
    51601 cggttattgc cgctggtacg gcgggaacgt gctcatgcag catcaaccct aatgccacgc
    51661 cgacaaacac tgccgggaag attcgcccac cgcgaaagcc actggcggca gcaacgacca
    51721 gggcggcaag tttaattacc gccagcaaaa agtaatcgct ggtgctgaaa gcctgatttg
    51781 ccaccatctg ctgcatctca tccagccctt taaacagcga aactggtcca ccaataaccc
    51841 ccagaatacc gagaataaat ccgccaatac ccagcacgag caccggattt ttcatttgat
    51901 gcatcattga gtgcaaccgt ggtaagcacc atacggcgac catccctgct gcgatggcaa
    51961 tcgccgcgac aattgcaccg ctgagaatat cggtcatttc catctgtccg taatgcgcaa
    52021 tgggcagtga aaaatgagga tggaaaaata atccggtagt aagtgcacca gctgctgccg
    52081 ccattaacgg cgcaaagaga cgatcccata gcggaacttc actactgcca tttaaggttt
    52141 gcgaaaatat caacgccgcc gcaacaggtg tgccaaacag cgcgccgatg gttccggcag
    52201 aggctaaaat agtccactcc attcggttga cgcgcggtaa cagacgagcg ccaatggcaa
    52261 ccgcaagggc gatattgacg gtcatgatcg gatgttctgg ccccaggctg acgccaccag
    52321 caagaccaag aattaatgct acgataagtc ctggtagcgc agagggcgga accggtgcgc
    52381 cgatcagcgg ttcacaggcg gggtctggtc cggcatgacc ctggctgaaa cggataacca
    52441 accccaccgc aatacccgtt agcgttaata caccgatgat ccagaggggt gaatcctggg
    52501 ctatccccag agttcccggc agtcgttgcc agagcaaatt ctgtaatgcc gaggcgattt
    52561 tcatcaccac aatcagaata agactggacg caatcccaat tgccacggcg gggagcgata
    52621 ataacaacat ggttctggct cgcggatgga gcatgttcta ttccttatgc ggggtcagat
    52681 acttagtttg cccagcttgc aaaaaggcat cgctgcaatt ggtgctgaaa cgataaagta
    52741 aatgtgtgac ccagatcgat atttacaggg agcctgcctt tccggcgttg ttgttatgcc
    52801 cccaggtatt tacagtgtga gaaagaatta ttttgacttt agcggagcag ttgaagaatg
    52861 acaaagtatg cattagtcgg tgatgtgggc ggcaccaacg cacgtcttgc tctgtgtgat
    52921 attgccagtg gtgaaatctc gcaggctaag acctattcag ggcttgatta ccccagcctc
    52981 gaagcggtca ttcgcgttta tcttgaagaa cataaggtcg aggtgaaaga cggctgtatt
    53041 gccatcgctt gcccaattac cggtgactgg gtggcaatga ccaaccatac ctgggcgttc
    53101 tcaattgccg aaatgaaaaa gaatctcggt tttagccatc tggaaattat taacgatttt
    53161 accgctgtat cgatggcgat cccgatgctg aaaaaagagc atctgattca gtttggtggc
    53221 gcagaaccgg tcgaaggtaa acctattgcg gtttacggtg ccggaacagg gcttggggtt
    53281 gcgcatctgg tccatgtcga taagcgttgg gtaagcttgc cgggcgaagg cggccacgtt
    53341 gattttgcgc cgaatagtga agaagaggcc attatcctcg aaatattgcg tgcggaaatt
    53401 ggtcatgttt cggcggaacg cgtgctttct ggtcctgggc tggtgaattt gtatcgcgca
    53461 attgttaaag ccgacaaccg cctgccagaa aatctcaagc caaaagatat taccgaacgc
    53521 gcgctggctg acagctgcac cgattgccgc cgtgcgttgt cgctgttttg cgtcattatg
    53581 ggccgttttg gcggcaatct ggcattaacg ctgggaacct ttggtggggt ttatattgct
    53641 ggcggtatcg tgccgcgctt ccttgagttc ttcaaagcct ctggtttccg tgccgcattt
    53701 gaagataaag gacgctttaa agaatatgtc catgatattc cggtgtatct catcgtccat
    53761 aacaatccgg gtcttctcgg ttccggtgca catttacgcc agaccttagg tcacattctg
    53821 taaatccttc ctgttatatc gggaggtaac tctcccgata atcttttaaa tcatacagtt
    53881 aattcaattt ttctttgtgt cccctcacaa ggccgacctg cgtcacactt ccgtacagcg
    53941 ggattaattc tccagtaaat gcattatttg tctggtaacg gcgatttgtt ttgcacgttc
    54001 ataatttcac tcgtcaacta tcactgaatg aggaattaac gatgagtaag aaactgattg
    54061 ccttatgtgc ctgcccaatg ggcctggctc acacctttat ggccgctcag gcgttggaag
    54121 aagcggcggt agaagccggt tatgaagtga aaattgaaac ccagggcgcg gacggtatcc
    54181 agaatcgcct gacggcgcag gatatcgccg aagcaaccat catcatccac tccgtggcag
    54241 ttaccccgga agataatgag cgtttcgaat cacgcgacgt ttatgaaatc actttgcagg
    54301 acgcaattaa aaacgctgcg ggcatcatca aagaaatcga agagatgatt gcctctgaac
    54361 aacagtaata ttcgacaggg ataattgtta tggccattaa aaaacgcagt gcaaccgttg
    54421 tgcctggcgc atccggtgcg gcagcggcag ttaagaatcc gcaggcctct aaaagcagct
    54481 tctggggtga actcccgcag catgtgatgt cagggatttc acgcatggtg ccaaccttaa
    54541 ttatgggcgg tgtgatcctc gctttcagcc agctgattgc ttatagctgg cttaaaattc
    54601 ctgctgagat cggcatcatg gatgctctta atagcgggaa attctccggt ttcgacctct
    54661 ccttacttaa atttgcctgg ctgtcgcagt ccttcggcgg cgtgttgttt ggttttgcca
    54721 ttccgatgtt tgctgctttt gtggcgaact ctatcggtgg caaactggcg ttcccggctg
    54781 gttttatcgg cgggttgatg tctacccagc cgacgcaact gctgaacttc gatcccagca
    54841 ctatgcaatg ggcgacctct tcgccggtgc cgtcaacctt cattggtgcg ctgattattt
    54901 ctatcgtcgc gggttacctt gtgaagtgga tgaaccagaa aatccagtta ccggatttcc
    54961 tgttagcgtt caaaaccaca tttttgctac cgattctttc cgccattttt gtcatgctgg
    55021 cgatgtacta cgtcatcacc ccctttggtg gctggatcaa cggcggtatc cgtactgtgc
    55081 tgactgccgc aggtgagaag ggcgcgctga tgtacgcaat ggggatcgcc gcggcgacag
    55141 caatcgacct tggtggcccg atcaacaaag ccgcaggttt cgttgccttc agctttacca
    55201 ctgaccacgt attaccggtc accgctcgtt ctatcgctat cgttattcct ccgattggtc
    55261 tgggtctggc gaccattatt gaccgtcgtt taaccggcaa acgcctgttc aacgctcaac
    55321 tttatccgca gggtaagacc gcgatgttcc ttgcctttat ggggattagt gagggtgcga
    55381 ttccgtttgc gctggaaagc cccatcaccg ccattccgtc gtatatggtc ggcgcgattg
    55441 tcggctcaac cgccgctgtc tggctgggcg cagtgcaatg gttcccggaa tccgctatct
    55501 gggcatggcc gctggtcact aacctgggcg tctatatggc ggggatcgcg ctgggggcag
    55561 tcataactgc gctgatggtg gtgttcctgc gtctgatgat gttccgtaaa ggcaaattgt
    55621 taatcgatag cctgtaagaa ggaaaatcca gatgacatta ctcgcttcgc tgcgcgactg
    55681 gcttaaggcg caacaactgg atgcagtgct tctctcctca cggcagaaca aacagccgca
    55741 tctggggatc tccaccggat caggctatgt ggttattagc cgtgaaagtg cgcacattct
    55801 ggtagattcg cgctatttcg ttgaggtgga agcccgtgcg caaggctacc agctgcattt
    55861 gcttgacgcg acgaacacgc ttaccactat cgtcaatcaa atcattgccg atgagcagtt
    55921 gcaaacgctc ggttttgaag gccagcaggt gagttgggaa accgcgcacc gctggaagtc
    55981 tgaactcaat gcgaaactgg ttagcgccac gccggatgtg ctgcggcaaa tcaaaacgcc
    56041 agaggaggtg gagataatcc gcctcgcctg tgggattgcc gatcgcggag cggagcatat
    56101 tcgccgcttt attcaggcgg ggatgagcga gcgcgagata gccgctgaac tggagtggtt
    56161 tatgcgccag cagggcgcag aaaaagcctc ttttgacacc attgtcgcca gcggctggcg
    56221 cggggcgctg ccgcacggca aagccagcga caagattgtt gcagcgggcg agtttgtcac
    56281 tctcgatttt ggtgcgctgt atcagggcta ctgctctgat atgacgcgca ccttgctggt
    56341 gaatggcgaa ggggtgagcg ccgaatctca cccgctgttt aacgtctacc agattgtcct
    56401 gcaggcacag ctcgcagcaa tctctgcaat tcgccccggc gtgcgctgcc agcaggttga
    56461 cgatgccgcg cgccaagtga ttaccgaggc aggttttagc cactatttcg gtcataacac
    56521 cggtcatgct atcggcattg aagttcatga agatccgcgt ttttcaccgc gggacaccac
    56581 gacgctacag ccaggcatgt tactgaccgt ggagccgggg atttatttgc cagggcaagg
    56641 gggcgtgcgc atcgaagatg ttgtgctggt taccccgcaa ggcgcagaag tgctctacgc
    56701 catgccgaaa acagtgttgc tcacgggaga ggcataatgg atttatcgct attaaaagcg
    56761 ttgagcgagg cagatgcgat cgcctcctcg gaacaggaag tgcggcagat cctgctggaa
    56821 gaagcggatc gcctgcaaaa agaagtgcga tttgatggtc tgggatcggt gctgatccgc
    56881 ctcaatgaat cgacaggtcc gaaggtgatg atctgcgccc atatggacga agtgggattt
    56941 atggtgcgta gcatctcccg cgaaggggca attgatgtgc tgccggttgg caacgtgcgc
    57001 atggctgccc gccagctgca gccggtgcgc atcaccaccc gtgaagagtg caaaattcca
    57061 ggcctgcttg acggcgaccg gcaggggaat gacgtcagcg ccatgcgcgt ggacattggt
    57121 gcgcgctcgt atgacgaagt gatgcaggcg ggaattcgtc caggcgatcg cgtcaccttt
    57181 gatacaactt ttcaggttct ccctcaccag cgggtgatgg ggaaagcctt tgatgatcgc
    57241 ctcggttgct atctgctggt gacgttacta cgtgagctgc acgatgccga actgcctgcg
    57301 gaggtgtggc tggtggcaag ttccagcgaa gaggtgggat tacgcggtgg gcaaaccgct
    57361 acccgcgcgg tgtcgccgga cgtcgccatt gtgctggata ccgcctgctg ggcgaaaaac
    57421 tttgattatg gcgcggctaa ccatcgccag attggtaacg gcccgatgct ggtgttaagc
    57481 gacaagtcac tgattgcgcc gccaaaactt accgcctgga tcgaaaccgt ggcggctgaa
    57541 attggcgtac cgttgcaggc agatatgttc agcaacggcg gcacggacgg cggagcggtg
    57601 cacttaaccg gtactggcgt acccacagtg gtaatggggc ctgccacccg ccacggacat
    57661 tgcgcggcat cgattgccga ttgccgcgac attttgcaga tggagcaact tttatctgcc
    57721 cttattcaac gtcttacgcg tgagacggtt gttcaactga cggatttcag atgatctcct
    57781 gattaacccg gagcggttat gttaacgatt caatttctct gtcctttgcc taacggtcta
    57841 catgctcgtc cggcgtggga acttaaagaa cagtgcagcc agtggcaaag cgaaatcacc
    57901 tttttcaacc atcgccagaa cgcaaaggcc gatgctaaaa gctcgctggc gttgattgga
    57961 actggcacct tatttaatga cagttgcagc ctgaacatta gcggcagcga tgaagagcag
    58021 gcgcggcgcg tgctggaaga gtacatcctg gtgcgcttta tcgacagcga cagcgttcag
    58081 ccgacgcagg cagaactgac ggcgcatccg ctgccgcgtt cattaagccg ccttaacccg
    58141 gatttactgt acggcaatgt gctggcaagc ggcgtcggcg tgggtacgct gaccctgtta
    58201 caaagcgaca gcctcgacag ttatcgggca atccccgcca gtgcgcaaga ttccacccgg
    58261 ctggagcaca gcctggcaac gcttgccgag caactgaacc agcaattgcg tgagcgtgac
    58321 ggcgaaagca aaactatcct cagcgcccat ttgtcgctga ttcaggatga tgaattcgcg
    58381 ggcaatatcc gtcgtctgat gacagaacag catcaagggc tgggggcggc gatcatcagc
    58441 aatatggagc aggtttgcgc caaactttct gcttctgcca gtgattatct gcgcgaacgt
    58501 gttagcgaca ttcgcgatat tagcgaacag ttgctgcata tcacctggcc ggaactgaag
    58561 ccgcgcaaca atctggtgct ggaaaaaccg actattctgg tagccgagga tttaacccca
    58621 agccagtttt tgagcctcga tttaaaaaat cttgcaggca tgattctgga gaaaactggg
    58681 cgcacctcgc ataccctgat tctggcccgc gcctcggcga tcccggtact gagcggcctg
    58741 ccgctggatg cgattgcccg ttatgccggg caaccggcgg tgcttgacgc ccagtgcggc
    58801 gtgctggcga ttaacccgaa tgacgcggtg agcggttatt atcaggtcgc acagacgctg
    58861 gcggataaac gccaaaaaca acaggcgcag gccgccgcgc agctggccta ttcccgtgat
    58921 aacaagcgta ttgatattgc ggcgaatatc ggcaccgctc tggaagcgcc aggcgcgttt
    58981 gccaacggcg cggaaggcgt tggactgttc cgtactgaaa tgctgtatat ggatcgtgac
    59041 agcgcgccgg acgagcagga gcaatttgaa gcctaccagc aggtactact ggcagcgggc
    59101 gacaagccga ttatcttccg cacaatggac atcggtggcg ataaaagcat tccttatctg
    59161 aatattcccc aggaagagaa cccgttcctc ggctatcgcg cggtacgtat ttacccggaa
    59221 tttgctggcc tgttccgcac tcaactacgg gcgattttgc gcgccgccag ttttggcaac
    59281 gcccagttga tgatcccgat ggtccacagt ctcgatcaga tcttatgggt gaagggcgag
    59341 atccaaaaag cgatcgttga gcttaagcgc gatggcctgc gtcatgcaga gacgattacg
    59401 ctggggatca tggtggaagt gccgtcggtg tgctacatca tcgatcactt ctgcgatgag
    59461 gtcgatttct tcagtatcgg ctccaacgat atgacccagt atctgtatgc ggtcgatcgt
    59521 aataacccgc gcgtatcgcc gctgtataac ccgattacgc catcgttcct gcgcatgttg
    59581 cagcagattg ttaccactgc gcatcagcgg ggcaaatggg taggcatttg cggtgaactg
    59641 ggcggtgaaa gccgttatct gccgctactg cttgggctgg gcctggatga gctgagtatg
    59701 agtagcccgc gtattccggc ggtgaaaagc cagcttcgtc aactggatag cgaggcgtgt
    59761 cgggaactgg cgcgtcaggc atgtgaatgc cgcagtgcgc aggaaattga agcgttactc
    59821 accgccttta cgccggaaga agacgttcgc ccactgctgg cgctggagaa tatctttgtt
    59881 gatcaggatt ttagcaataa agagcaggcg atccagttcc tgtgcggcaa cctcggcgtt
    59941 aacgggcgca ctgaacatcc gtttgagctg gaagaagatg tctggcagcg ggaagagatt
    60001 gtcactaccg gcgttggttt tggcgtggcg atcccgcaca ccaaatctca gtggatccgt
    60061 cattccagta tcagcattgc ccggctggtg aaaccggttg actggcagtc agagatgggc
    60121 gaagtcgaac tggtgatcat gctgacgttg ggtgctaacg aagggatgaa ccatgtgaaa
    60181 gtcttctcgc agctggcgcg taaactggtg aataaaaact tccgccagtc attgtttgcc
    60241 gcgcaagatg cgcaaagcat cctgacgctg ctggaaacgg aattaacctt ctgacgttag
    60301 ccctgaaaac gggcgctgta ctctcccggc gtcagaccaa actgacgccg gaaaacgcga
    60361 caaaaatagt cgctgtccgg aaaaccgcaa cgctgtgcca cttcatgaat tgacagatga
    60421 tatttctgca aaatcatcct cgccttcgcc attcgcaccc aacgcacata ctcgataaaa
    60481 cgcatcgttc catgctgagc aaacagtttt gacagatgat tgggcgtgat attaaaaaac
    60541 tgcgccacgc tctcgcgggt gagcggctgg gcataattat cctgaaccca gttacaaatg
    60601 ctgtgataga gaaaatcgcc gcgcggttgc gaattacccg gcggcatatt caccacgttg
    60661 cggcaaagat gcagcaaact taacaccagt ggctgaatga tattctgctc cagcggcgaa
    60721 cggctaagat gggtgagggc ggtaagcatc gcttcacctt caccgcgttg caggctggga
    60781 agctggatct ggcgagcagg atgcaacaac gacgtggtgc ggctatcgta aaacgataac
    60841 ccaagccagg tcggtgcgaa caccaggctt aacagcatca ccggtttgtt gttgatcggt
    60901 aaattagccg cgcgtgccgg aataaacagc atttctcctt ccaccattcg ctgctgcttt
    60961 gcttccagca ggttgccgta ttcgccgcgc agcacgatat ccagacgggg aaaatcaata
    61021 cataaactgc ccaccggtaa cgaggcaggc tggctggcaa accagacgcg ccccagtagt
    61081 tgcgggttaa gcaccaggcc gctgaacaga tcggcgaaaa attgttgatc ggcgggcaag
    61141 cctggtgctt tcatgaaagt tcccgattaa agatgcatta actggcgaaa ttctttcact
    61201 ttgctgcggc tgaccggcac ttcaaaatcc agatctttca gtcgcagaat gtaggtgtta
    61261 ttaaaccacg gttcgatttc gcgtattttg ttcagattga cacaaaatga gcgatggcag
    61321 cggaaaaaat gcgacggcgg cagtttgctg caaaattcgg taatgttcat cggcattacg
    61381 taggattcac gccgcgtata gacaaaggtc attttctcgt gcgcttcggc gtaatagata
    61441 tcgttaattg gcgtgacgat tattcgctca tctttcacca gattaatggt gtcattctca
    61501 cgcgttaccg tcgcggcagg cgtactgctg gtctgctgtt gttgccaggc cgcttccagt
    61561 ttttgcagca tcccggtaat acgtgactcc tgatacggtt tgagaatgta gtcaaacgcc
    61621 tccagttcaa acgcttctac cgcatgttct ttccacgcgg tgatgaacac aataaacggt
    61681 ttatgggcga actggctgat gttttgcgcc agcaacacgc catccagcga cggaatattg
    61741 atatccagaa aaatggcgtc gacgcggtta tgctgcaaaa acttcaacac gtccagaccg
    61801 tcgtcaaagg tgccgacaat ctccatctgg ctgtgctctt taattagcca gctcagttcc
    61861 tgttgtgcca ggaattcgtc ttcaacaatg atgactttca caatatcact ccggctcaaa
    61921 gcaataacgt agcctgtgag gcgactgggg tgcgttggtt aggaatgtaa aacgcaattt
    61981 ccgtccccgg ctccaggcgg cggatatgca gcccctcgcc atacaataac ttcacgcgat
    62041 gatggacatt cagcaggcca attttattgc ccggcatttc attcgcttcg acccgctcaa
    62101 tcacctttgg atcgatgccg tgcccggtat ctcgcaccgc aatgcgtacc cgatttccgc
    62161 actctgcaac gctgatggtg acaacgcctt tacctttgca aggctgaata ccgtggacaa
    62221 tggcgttctc caccaacggc tggatcagca ggctgggaat gcagcaattc acctcttcat
    62281 caatatcata gataaccgtc agcttgtcac caaagcgggc ctgctcaatg gcaatatagt
    62341 ctttaatttg atacagctct tttttgatat cgatttgctc atcgtctttt aattcaatgt
    62401 tatagcgcag ataacgcgac agattaaaga tcaactggcg agcggtatcc ggattcagac
    62461 ggattgacga tgaaatagcg ttcagagcgt taaacagaaa atggggatta attttgcttt
    62521 gcagggcgcg aagctctgcc ttatttgcca tttcacgtag ctgctcggcg cgtgaaacct
    62581 ccagttgcgt ggagatgatt tgcgacagac cgaccgccat ttcctgtaat gacgaggtga
    62641 tctgatgcgc gtggcagtag taaattttca gcgttccggt cacgacccct ttctcccaca
    62701 atgggatcac cagcatggaa tgaatctctg gtgtgcggtg ggcttcatca ttgtttttaa
    62761 tgatgatttt tccgtaattc atcgcctgac gggtagtcgg gctaatgaag tcatcgccat
    62821 tctgatagtt atgttcaccc acgccaacat aggccagcac atgatcggta ttggtaattg
    62881 ccaccgcatc ggcgtgaatg tcatcgcgga taatttcgca gaccttgcgt aatgactcgc
    62941 tattgacatg gcgaaacagc ggtagcgttt tgttggcgat atccagcgcc agcttggcct
    63001 gccgcgcggc gctggcctct ttttcgccct caacgctttg caccagaagc acaataaagc
    63061 cgatacagac gctaccgagg atcattggaa tgccgatttt agagacgata tcgatcccca
    63121 acgcggtagt tggtgcccag acaattacca gaatcatggt caacgtctca cacaacatgc
    63181 cgccgagaat accgacgcgc cagcgctgtg ctttggggat tttcaggttg atccagcccg
    63241 atatacaacc cgccagaatg ctggtgataa agcaggggat cgccgtcacg ccgccgatat
    63301 caattaaata ccggtgaata cccgcaatca cgccggtaat gatgcctacc cacgggccaa
    63361 acagaatccc gccggacatc accgcgataa tacgtacgtt caccagcgag ccttctacgg
    63421 gaacgccgga ccaggtgctg aacagggcga acagcgagaa aatggcggta acggcgagca
    63481 attcttttgg ggagtgcgcc gacttgtgca acagttcgcg aaacaggcgg atacggatga
    63541 gaaagaacag gcagataagc attaacgctg cccgatcgaa gaccgccagc agcatgttga
    63601 atatttcgtg cacgggtgac tctgaataag gattaaacct ctatgatagg taacctgaag
    63661 gctgatgacc agcaggccgt ttttgaggaa aaaccataaa tttaaaaggg gatt
//
LOCUS       DAJOWB010000014        61801 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJOWB010000014 DAJOWB010000000
VERSION     DAJOWB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.014"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            199..870
                     /locus_tag="OHO85_001010"
     CDS             199..870
                     /locus_tag="OHO85_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HCQ2670424.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            880..2076
                     /locus_tag="OHO85_001011"
     CDS             880..2076
                     /locus_tag="OHO85_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCQ2670425.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYSQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2054..2635
                     /locus_tag="OHO85_001012"
     CDS             2054..2635
                     /locus_tag="OHO85_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCQ2670426.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2637..3410
                     /locus_tag="OHO85_001013"
     CDS             2637..3410
                     /locus_tag="OHO85_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HCQ2670427.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3595..3870
                     /gene="frmR"
                     /locus_tag="OHO85_001014"
     CDS             3595..3870
                     /gene="frmR"
                     /locus_tag="OHO85_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HCQ2670428.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3905..5014
                     /gene="frmA"
                     /locus_tag="OHO85_001015"
     CDS             3905..5014
                     /gene="frmA"
                     /locus_tag="OHO85_001015"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HCQ2670429.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5107..5940
                     /gene="fghA"
                     /locus_tag="OHO85_001016"
     CDS             5107..5940
                     /gene="fghA"
                     /locus_tag="OHO85_001016"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCQ2670430.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6065..6604)
                     /locus_tag="OHO85_001017"
     CDS             complement(6065..6604)
                     /locus_tag="OHO85_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HCQ2670431.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6706..7917)
                     /gene="mhpT"
                     /locus_tag="OHO85_001018"
     CDS             complement(6706..7917)
                     /gene="mhpT"
                     /locus_tag="OHO85_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HCQ2670432.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8093..9106)
                     /gene="dmpG"
                     /locus_tag="OHO85_001019"
     CDS             complement(8093..9106)
                     /gene="dmpG"
                     /locus_tag="OHO85_001019"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HCQ2670433.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVFAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9103..10053)
                     /gene="mhpF"
                     /locus_tag="OHO85_001020"
     CDS             complement(9103..10053)
                     /gene="mhpF"
                     /locus_tag="OHO85_001020"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HCQ2670434.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPKSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10050..10859)
                     /gene="mhpD"
                     /locus_tag="OHO85_001021"
     CDS             complement(10050..10859)
                     /gene="mhpD"
                     /locus_tag="OHO85_001021"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HCQ2670435.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10869..11735)
                     /gene="mhpC"
                     /locus_tag="OHO85_001022"
     CDS             complement(10869..11735)
                     /gene="mhpC"
                     /locus_tag="OHO85_001022"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HCQ2670436.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLKWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11753..12697)
                     /gene="mhpB"
                     /locus_tag="OHO85_001023"
     CDS             complement(11753..12697)
                     /gene="mhpB"
                     /locus_tag="OHO85_001023"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HCQ2670437.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12699..14363)
                     /locus_tag="OHO85_001024"
     CDS             complement(12699..14363)
                     /locus_tag="OHO85_001024"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HCQ2670438.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTTGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14440..15387
                     /gene="mhpR"
                     /locus_tag="OHO85_001025"
     CDS             14440..15387
                     /gene="mhpR"
                     /locus_tag="OHO85_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HCQ2670439.1"
                     /translation="MIFYCALSIGRVFSATIKTCPNVHQVHNVVLTIEMSINMQNNEQ
                     TEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLQEEGYVRRS
                     PSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAMVVRE
                     TTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQERKELIEMLAARPGDDYQLAR
                     EPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMASAMTI
                     EQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHLR"
     gene            15464..16546
                     /gene="lacI"
                     /locus_tag="OHO85_001026"
     CDS             15464..16546
                     /gene="lacI"
                     /locus_tag="OHO85_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HCQ2670440.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16669..19743
                     /gene="lacZ"
                     /locus_tag="OHO85_001027"
     CDS             16669..19743
                     /gene="lacZ"
                     /locus_tag="OHO85_001027"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HCQ2670441.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHIIPQ
                     LTTSETDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19795..21048
                     /gene="lacY"
                     /locus_tag="OHO85_001028"
     CDS             19795..21048
                     /gene="lacY"
                     /locus_tag="OHO85_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HCQ2670442.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTVNNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21114..21725
                     /gene="lacA"
                     /locus_tag="OHO85_001029"
     CDS             21114..21725
                     /gene="lacA"
                     /locus_tag="OHO85_001029"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HCQ2670443.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLLKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRDKQYYFKDYKVESSV"
     gene            complement(21828..23009)
                     /gene="cynX"
                     /locus_tag="OHO85_001030"
     CDS             complement(21828..23009)
                     /gene="cynX"
                     /locus_tag="OHO85_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HCQ2670444.1"
                     /translation="MRNHQKTFTILLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAALTPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(23015..23485)
                     /gene="cynS"
                     /locus_tag="OHO85_001031"
     CDS             complement(23015..23485)
                     /gene="cynS"
                     /locus_tag="OHO85_001031"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HCQ2670445.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23516..24175)
                     /gene="cynT"
                     /locus_tag="OHO85_001032"
     CDS             complement(23516..24175)
                     /gene="cynT"
                     /locus_tag="OHO85_001032"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HCQ2670446.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            24285..25184
                     /gene="cynR"
                     /locus_tag="OHO85_001033"
     CDS             24285..25184
                     /gene="cynR"
                     /locus_tag="OHO85_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HCQ2670447.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIDFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(25224..26507)
                     /gene="codA"
                     /locus_tag="OHO85_001034"
     CDS             complement(25224..26507)
                     /gene="codA"
                     /locus_tag="OHO85_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HCQ2670448.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(26497..27756)
                     /gene="codB"
                     /locus_tag="OHO85_001035"
     CDS             complement(26497..27756)
                     /gene="codB"
                     /locus_tag="OHO85_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HCQ2670449.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(28088..29974)
                     /gene="prpE"
                     /locus_tag="OHO85_001036"
     CDS             complement(28088..29974)
                     /gene="prpE"
                     /locus_tag="OHO85_001036"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HCQ2670450.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(30014..31465)
                     /gene="prpD"
                     /locus_tag="OHO85_001037"
     CDS             complement(30014..31465)
                     /gene="prpD"
                     /locus_tag="OHO85_001037"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HCQ2670451.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(31499..32668)
                     /gene="prpC"
                     /locus_tag="OHO85_001038"
     CDS             complement(31499..32668)
                     /gene="prpC"
                     /locus_tag="OHO85_001038"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HCQ2670452.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(32713..33603)
                     /gene="prpB"
                     /locus_tag="OHO85_001039"
     CDS             complement(32713..33603)
                     /gene="prpB"
                     /locus_tag="OHO85_001039"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HCQ2670453.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEAKLDDLFARSQVK"
     gene            33841..35427
                     /gene="prpR"
                     /locus_tag="OHO85_001040"
     CDS             33841..35427
                     /gene="prpR"
                     /locus_tag="OHO85_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HCQ2670454.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMQQGRFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(35525..35800)
                     /locus_tag="OHO85_001041"
     CDS             complement(35525..35800)
                     /locus_tag="OHO85_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCQ2670455.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            35947..36618
                     /locus_tag="OHO85_001042"
     CDS             35947..36618
                     /locus_tag="OHO85_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCQ2670456.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(36635..36880)
                     /locus_tag="OHO85_001043"
     CDS             complement(36635..36880)
                     /locus_tag="OHO85_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670457.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTVES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMVGIRQIRLIT"
     gene            complement(37292..38107)
                     /locus_tag="OHO85_001044"
     CDS             complement(37292..38107)
                     /locus_tag="OHO85_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670458.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYIDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(38350..39399)
                     /gene="yahK"
                     /locus_tag="OHO85_001045"
     CDS             complement(38350..39399)
                     /gene="yahK"
                     /locus_tag="OHO85_001045"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HCQ2670459.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(39776..41158)
                     /locus_tag="OHO85_001046"
     CDS             complement(39776..41158)
                     /locus_tag="OHO85_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HCQ2670460.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDELAHRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(41168..42118)
                     /locus_tag="OHO85_001047"
     CDS             complement(41168..42118)
                     /locus_tag="OHO85_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HCQ2670461.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(42261..43679)
                     /locus_tag="OHO85_001048"
     CDS             complement(42261..43679)
                     /locus_tag="OHO85_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCQ2670462.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(43679..45226)
                     /gene="fdrA"
                     /locus_tag="OHO85_001049"
     CDS             complement(43679..45226)
                     /gene="fdrA"
                     /locus_tag="OHO85_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCQ2670463.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(45216..46079)
                     /locus_tag="OHO85_001050"
     CDS             complement(45216..46079)
                     /locus_tag="OHO85_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCQ2670464.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFNGLFRHGDKVQFDEQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLALVLFIPGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHSFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(46119..46724)
                     /locus_tag="OHO85_001051"
     CDS             complement(46119..46724)
                     /locus_tag="OHO85_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HCQ2670465.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDLTCLNPFLMSCLNGDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            46982..47479
                     /locus_tag="OHO85_001052"
     CDS             46982..47479
                     /locus_tag="OHO85_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCQ2670466.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGISGFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTMFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            47571..48503
                     /locus_tag="OHO85_001053"
     CDS             47571..48503
                     /locus_tag="OHO85_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2670467.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(48545..49633)
                     /gene="pdeL"
                     /locus_tag="OHO85_001054"
     CDS             complement(48545..49633)
                     /gene="pdeL"
                     /locus_tag="OHO85_001054"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator/c-di-GMP
                     phosphodiesterase PdeL"
                     /protein_id="HCQ2670468.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGSNSHRYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFVSEWVMKAGG"
     gene            complement(50128..50202)
                     /locus_tag="OHO85_001055"
     CDS             complement(50128..50202)
                     /locus_tag="OHO85_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670469.1"
                     /translation="MCDILLNVLNIVFIGIAIILVIIC"
     gene            complement(50508..52541)
                     /gene="betT"
                     /locus_tag="OHO85_001056"
     CDS             complement(50508..52541)
                     /gene="betT"
                     /locus_tag="OHO85_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCQ2670470.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            52670..53257
                     /gene="betI"
                     /locus_tag="OHO85_001057"
     CDS             52670..53257
                     /gene="betI"
                     /locus_tag="OHO85_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HCQ2670471.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            53271..54743
                     /gene="betB"
                     /locus_tag="OHO85_001058"
     CDS             53271..54743
                     /gene="betB"
                     /locus_tag="OHO85_001058"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HCQ2670472.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSALQGQKIWASMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            54757..56427
                     /gene="betA"
                     /locus_tag="OHO85_001059"
     CDS             54757..56427
                     /gene="betA"
                     /locus_tag="OHO85_001059"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HCQ2670473.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDMGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            56640..57308
                     /locus_tag="OHO85_001060"
     CDS             56640..57308
                     /locus_tag="OHO85_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670474.1"
                     /translation="MREQIKQDIDLIEILFYLKKKIRVILFIMAICMAMVLLFLYINK
                     DNIKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIR
                     LEWSGDKRALPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(57551..58246)
                     /locus_tag="OHO85_001061"
     CDS             complement(57551..58246)
                     /locus_tag="OHO85_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCQ2670475.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVILFSAAERGRSLSLLP
                     EYSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(58239..59666)
                     /locus_tag="OHO85_001062"
     CDS             complement(58239..59666)
                     /locus_tag="OHO85_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCQ2670476.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFARTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDNVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(59677..60396)
                     /locus_tag="OHO85_001063"
     CDS             complement(59677..60396)
                     /locus_tag="OHO85_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCQ2670477.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPMYLADEPEWASRAA
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELFTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(60924..61778)
                     /gene="rclR"
                     /locus_tag="OHO85_001064"
     CDS             complement(60924..61778)
                     /gene="rclR"
                     /locus_tag="OHO85_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HCQ2670478.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRLAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT        14812 a        16615 c        15636 g        14738 t
ORIGIN      
        1 cataccaaac atacaaacac ttaacatatg aattgcatag aaacattacc cagtcgaacc
       61 tccgggtctg aaagcccttg cttttcagtt attagcaaca tttaacattt ataaattaac
      121 atctggctgc gccagagatt acgtctctga gtgcatcgtt gtgcttgtag tataataaat
      181 aaaatggagt gtcattaagt ggataaggtt ttagattcag ccctcctttc ttcggcaaat
      241 aaaagaaagg gtatccttgc tattggcgca catcctgacg atatagaatt aggctgtggc
      301 gcatcgcttg ctcgtcttgc gcaaaaagga atttatatcg cagccgtggt gatgactacc
      361 ggcaactctg gcacagatgg aataatcgat cgccatgaag aatcgcgcaa cgccctaaag
      421 atattagggt gccaccaaac tattcatctt aattttgctg acacccgcgc tcatttacag
      481 ctcaatgata tgatttccgc cctggaagac atcattaaaa atcaaattcc ttctgatgtt
      541 gaaatcatgc gggtatatac catgcatgat gccgaccgcc atcaggatca tctcgctgtt
      601 tatcaagctt caatggttgc ctgccgcact attccacaaa ttctcggcta cgaaacccca
      661 agtacctggc tttcatttat gcctcaggtt tttgaatccg ttaaagaaga atatttcaca
      721 gtcaaacttg ctgcattaaa aaaacataaa agccaggaac gacgcgatta tatgcgtcat
      781 gatcgcctgc gtgcagttgc acaatttcgc gggcaacagg tcaatagcga tctgggtgaa
      841 ggctttgtaa ttcataaaat gattctttga gcaaattgca tgaaaacctg gatatttatc
      901 tgtatgtcca tagcaatgtt gctatggttt ttaagtacgc taagacgtaa acccagtcaa
      961 aagaaaggct gtattgacgc cattatacct gcgtataacg aaggcccgtg tctggcgcag
     1021 tcactggata atctactgcg taacccttat ttttgccggg taatttgcgt taacgacggc
     1081 tccacggaca ataccgaagc ggtcatggcg gaagtcaaac gcaaatgggg cgaccgcttt
     1141 gttgccgtca cgcaaaaaaa taccggtaaa ggtggtgcgc tgatgaatgg cctcaattat
     1201 gccacctgcg accaggtttt tttaagtgat gccgacacct atgttccgcc cgatcaagac
     1261 ggaatgggct atatgctggc agaaattgag cgcggtgctg atgccgtagg cggcattccc
     1321 tctactgcgt tgaaaggcgc ggggctgtta ccgcacatcc gcgcgaccgt aaagttgccg
     1381 atgattgtta tgaagcgcac gctacagcag ctcctgggcg gcgcaccgtt tattatcagc
     1441 ggtgcctgcg gaatgttccg cactgatgta ttgcgtaagt tcggtttctc agatcgtact
     1501 aaagtcgaag accttgatct cacctggaca ttagtggcaa acggctaccg tattcggcag
     1561 gcgaatcgct gcatcgtata ctcacaggaa tgcaacagcc cgcgtgagga gtggcgtcgc
     1621 tggcggcgtt ggattgtggg atacgcggtc tgtatgcgcc tgcataaaag acttttattt
     1681 agccgcttcg gtatcttcag tatatttcct atgctgttgg ttgtgattta tggcgttggg
     1741 atttatctca ctacctggtt taatgaattc atcaccaccg ggccgcatgg tgtggtgttg
     1801 gcaatgtttc cgcttatctg gatcggcgta gtttgtgtta ttggtgcttt tagcgcctgg
     1861 tttcatcgtt gctggttgtt ggtgccttta gcgccgcttt ccgttgtgta tgtattatta
     1921 gcttatgcca tctggattat ttatggactt attgcctttt ttactggacg cgaacctcag
     1981 cgcgacaaac ccacccgcta ttccgcactg gtggaagcgt caaccgctta ttcccaacct
     2041 tctgtcacag gaactgaaaa actatctgaa gcttaatgtt aaagagaaaa atattcagat
     2101 tgcagaccag gtaattattg atgaaactgc aggtgaagtt gttatcggcg cgaatacccg
     2161 tatttgtcat ggtgccgtta ttcagggtcc ggtagtgatt ggcgcaaact gcctgatagg
     2221 taattatgcg tttattcgtc ctggcacaat aatcagcaat ggtgtaaaaa ttggttttgc
     2281 caccgaaatt aaaaatgcgg ttattgaagc ggaagcaacg attggtccgc aatgttttat
     2341 tgccgactcg gtagttgcaa accaggcata tttgggcgca caagtacgta ccagtaatca
     2401 tcgtctggat gaacagcctg tgtctgttcg aactccagag ggaattatcg ctaccggatg
     2461 cgataaatta ggttgttata tcgggcagcg ttcacgcctt ggcgtacaag ttataatttt
     2521 gcctgggcga attatttccc cgaacacgca acttggcccg cgcgtgattg tagaacgtaa
     2581 tttacctgcc ggaacttatt cactccgaca agaacttatc cgtacaggag attaaaatga
     2641 taaaacggac gttattagcg gcggccattt ttagcgcatt gcccgcttat gccgggttaa
     2701 cttccattac cgcgggctac gattttaccg attattctgg cgatcatggc aaccgtaatt
     2761 tagcgtatgc tgaactggtg gcgaaagttg aaaacgcaac gctgcttttt aatctttccc
     2821 aggggcgtcg tgattatgaa actgaacatt tcaatgccac tcgcggtcag ggtgcggtct
     2881 ggtataaatg gaataactgg ctgacaaccc gaacgggtat tgcctttgcg gataatacgc
     2941 cggtctttgc ccgccaggat tttcgtcagg atattaacct ggccctgttg ccaaaaacgc
     3001 ttttcacgac cggttatcgc tacactaaat attacgatga tgtcgaagtc gatgcctggc
     3061 aaggcggcgt atcactctat actggcccgg tgatcaccag ctaccgctat acccattatg
     3121 actccagcga tgcaggtggt agttatagca atatgatttc cgtgcgtctg aatgacccgc
     3181 gcggcactgg ttatacgcaa ctatggctaa gccgcggaac aggcgcttac acctatgact
     3241 ggacgccaga aacacgctac ggcagcatga agagcgtcag tttgcaacgt attcaaccgc
     3301 tgactgagca acttaatctc gggctgacgg caggtaaagt gtggtacgac accccaaccg
     3361 atgattataa cggtctgcaa cttgcagccc atctgacctg gaaattctga ttccttctgc
     3421 cgcccgctat ccggggcggc cttccctgcc gattagcccc ccctttcctc tttgttttcc
     3481 gaccacattc accggataaa ttttattctc cagtgttata tactataggg gggtatgcat
     3541 tgacatatag catacccccc tatagtatat tgcgtgcaga tgatgaggtg cgaaatgccc
     3601 agtactccgg aagagaagaa aaaggtcctt actcgagttc gtcgtattcg agggcagatt
     3661 gatgctctgg aacggtcgct ggagggtgat gccgaatgcc gtgccatact ccaacagatc
     3721 gctgccgttc ggggcgcggc taacgggctg atggcagaag tgcttgaaag ccatatccgg
     3781 gaaacgtttg accgaaatga ctgctacagc cgcgaagtca gccaatccgt tgacgacact
     3841 attgaactgg ttcgagccta tcttaaatag ctgaatctat taccatattg aggaagagcg
     3901 agagatgaaa tcacgtgctg ccgttgcatt tgctcccggt aaaccgctgg aaatcgttga
     3961 aattgacgtt gcaccaccga aaaaaggtga agtgctgatt aaagtcaccc ataccggcgt
     4021 ttgccatacc gacgcattta ccctctccgg ggatgacccg gaaggtgtat tcccggtggt
     4081 tctcggtcac gaaggggccg gcgttgtggt tgaagtcggt gaaggcgtaa ccagcgtcaa
     4141 acctggcgac catgtgatcc cgctttacac cgcggagtgc ggcgagtgtg agttctgtcg
     4201 ttctggcaaa actaacctct gtgttgcggt tcgcgaaacc cagggtaaag gcttgatgcc
     4261 agacggcacc acccgttttt cttacaacgg gcagccgctt tatcactaca tggggtgctc
     4321 tacattcagt gaatacaccg tggtcgcgga agtgtctctg gccaaaatta atccagaagc
     4381 aaaccatgaa cacgtctgtc tgctgggctg tggcgtgacc accggtattg gcgcggtgca
     4441 caacacagct aaagtccagc caggtgattc tgttgccgtg tttggtcttg gcgcgattgg
     4501 tctggcagtg gttcagggcg cgcgtcaggc gaaagcggga cggattatcg ctatcgatac
     4561 caacccgaag aaattcgatc tggcgcgtcg cttcggtgct accgactgca ttaacccgaa
     4621 tgactacgac aaaccgatta aagatgtcct gctggatatc aacaaatggg gtatcgacca
     4681 tacctttgaa tgcatcggta acgtcaacgt gatgcgtgcg gcgctggaaa gtgcgcaccg
     4741 cggctggggt cagtcggtga tcatcggggt ggcaggttct ggtcaggaaa tctccacccg
     4801 tccattccag ttggtcactg gtcgcgtatg gaaaggttcc gcgtttggcg gcgtgaaagg
     4861 tcgttcccag ttaccgggta tggttgaaga tgcgatgaaa ggtgatatcg atctggaacc
     4921 gtttgtcacg cataccatga gccttgatga aattaatgac gccttcgacc tgatgcatga
     4981 aggcaaatcc attcgaaccg taattcgtta ctgatttccc gcaggtatac cccgtccact
     5041 tcagacgggg ttcttaatac tctccccggg cagccgtccg ggggattaac cgtgagataa
     5101 tgactgatgg aactcattga aaaacatgcc agctttggcg gctggcaaaa tgtgtatcgg
     5161 cattattccc aatcactgaa atgtgaaatg aatgtcggcg tctatctccc accaaaagcc
     5221 gcgaatgaaa aattgccggt gctgtactgg ctttcgggcc tgacctgcaa cgagcagaat
     5281 ttcattacta aatcggggat gcagcgttac gcggctgagc acaacattat tgttgttgcg
     5341 ccggacacca gtccgcgagg cagtcatgtc gcagatgccg accgttacga tctcgggcaa
     5401 ggtgccgggt tttacctgaa cgcaacgcaa gcgccgtgga atgaacatta caaaatgtat
     5461 gactatatcc gcaacgagct gccggattta gtgatgcaac attttccggc aacgaccaga
     5521 aagtctatct ctggtcattc tatgggcggg ctgggcgcgc tggtgctggc gttacgtaac
     5581 ccagatgaat atgtcagcgt ctcggcgttt tcgcccattg tctccccatc gcaagtaccg
     5641 tggggacagc aagcctttgc cgcatatctt ggtgaaaata aagatgcctg gttggattac
     5701 gatccggtga gtcttatttc acaaggtcaa cgcgttgcgg aaatcatggt tgatcagggg
     5761 ttgagtgatg atttttacgc ggaacagctg cggactccaa atcttgaaaa gatctgccag
     5821 gagatgaata tcaagacgtt aatccgttat cacgagggtt acgaccacag ctattatttt
     5881 gtctccagtt ttattggcga gcatattgcc taccacgcca ataaactgaa tatgcgttga
     5941 taatagtgca cgactgccgg atgcggcgtg aacgccttat ccgcctacat ttcgcccgca
     6001 ggccgtaggc atgataagac gcgcatagcg tcgcatcagg catctgcgca cgacttatct
     6061 atgtttacca catcaaatca tcaggcactt tgaagtcggc atacggatcg tcttcatcct
     6121 gctcttcggc actgagcgcg ctgtgcaaca ctatactgct ggcatcacgc tgagctattt
     6181 tatcggcaac gctggcggga ataatggcgt attcaccttc gctattgtta tcgaccaaca
     6241 agcgagcaat cgccagacga ccattaatca actgcgcctg tgtgagctta tcgacaaaaa
     6301 tcttcttaat cagattgccg tcagtgaagt taaaaccaat atcgccattg gcaatggtga
     6361 ttcggttcat ttcaatgagc tgcttcacct gagctttata ttctttcgcc aacgcagctt
     6421 gtttttgttg ctcgctaagc tgtttatcac gctcaagctg tgccttttta ttttcttcta
     6481 ccgccgcccg agcttcacgc gcctgaacgc gcgatttttt cgccgttctc tccaccttcg
     6541 ccgctttttt gctggtgact aatcctgctt tgagcaactg ctcttgtaag gtaagttttg
     6601 ccatctttgt ttccaaaccc gctgaataat ttttagcgag tatacctgta aacgccgtgg
     6661 gtgtattggg ggcaagtccc tttcgcccct gctaaataag gcgcgtcagg catcggcaca
     6721 cggctgcatt cttgatctcc gactcatcaa aataaacacc gccaacccag caacaagaat
     6781 acccggtgca gaagcggcca ttacgccgac cgtgccagtg cctaatgcca gcattttccc
     6841 ggccagtaac ggaccgctca tagcccccag acgccctacc gccacggctg ttcccacacc
     6901 tgttgcgcgg atctgcgaac tgtaaaacaa cggtgccagg gcatacaaaa cgctttgccc
     6961 acctgtcgca aacaaccccg cgacaaatcc cgccagcaac ataccgttaa atgacgacac
     7021 cgttccaagc gccagcagcg aagctaacat gccgctataa atcagtagcg acatggttac
     7081 tggacgcagc ttatccatca atgcgcccaa cattaacgtc ccgcttgccg cccccatttg
     7141 cagggcaaac atcacccctg ccgcctgcga tggctggaat ccttgctcca ccaaaagtag
     7201 cggtagccag ttgatcaaca tataaaccac cagcagagtg aagaaataac acaaccacag
     7261 cagcagcgtc gcggttgccg tttctggcgc aaataaggca cgcagtggtg gcgcagcctg
     7321 tttttcgcca gcgaaaaccg ctgactccgg cagccagcgc attaatagcg gcaccagaat
     7381 caacggcacc acaccaccta cccaaaacac cgtttgccat gctaagtttg cccccgcgaa
     7441 acccagtgtc gccgccagcg ccgcgccaat gggaacaccg caatacatca ggctcactgc
     7501 cgtcccacga aaacgtggac ccgcggcttc agacgtcaga gcgataagat tcggcaacgc
     7561 cgcccccagc ccgacaccgg tcatcagccg cgcaaagacc agtgagggga aatcccaggc
     7621 aatcgccgtt gccagtgaga acaaaccaaa cagcgcaact gagccaatca aaatacgctt
     7681 acgaccataa cggtctgcca gcatcccgcc aaccaacgcg ccgggtagca aaccgagtat
     7741 tccggcgcta aatatccagc ccatttgcat tttatcgagt gcgaaagcct gggcgatgcc
     7801 acccgccgca atgccagccg cctgaagatc cagcccttcc atcagagcga ccaaaaaaca
     7861 aagcccgatg gtcagcatca ggcgggatga agatgatgaa ggggtacgag tcgacataag
     7921 atgaacctca ttgcgagtgc aggtgaatcg cgactgatac attgccagcc gcgtaatcag
     7981 aactatttaa tgactgccta cgggcgaaat gtaggccgga taaggcgttc acgccgcatc
     8041 cggctgtcgt gctgggatgg gcatacaaaa tgcacgttat cggcagtcat cattatttgt
     8101 tgttgcgcag atccagcgcc acgtcgacga tcatatcctc ctggccgcca accatccggc
     8161 gtttgcccag ctcaacgaga atatccaccg cacttaagcc ataacgcgcc gccgccgttt
     8221 cacagtgacg caggaagctc gagtaaacac cagcgtatcc cagcgccagc gtttcgcgat
     8281 cgactcgtac cggtcgatcc tgcaacggac gcaccaggtc gtcggcggca tccattaacg
     8341 catagagatc ggtcccatgc tgccagccca gtttatccgc ggcggcaata aacacttcca
     8401 gcggtgcgtt acctgcgccc gcgcccattc ccgcgaggct ggcgtcgatt cggtcgcagc
     8461 cctcttccac cgccgcgata gagttcgcca cgccaagact caggttatgg tgagcgtgca
     8521 tgccagtttg cgtttctggt ttcagctctg ctttcagggc gcggaaacgg tcacggatat
     8581 cgctcatgtt catcgcaccg ccagaatcca ccacataaat acaggtcgca ccgtagcctt
     8641 ccatcagctt tgcctgcttg gcgagattct ccggcgtggt catatggctc atcatcagaa
     8701 aaccaacggt gtccattccg agctcgcggg catactgaat atgctgggcg aaaacatcag
     8761 cttcggtaca gtgcgttgcc acacgaacca cccgcgcgcc agcctgccag gcatttttca
     8821 gatcgtgaat agtgccgatt cctggcagca acaacgtcgc gattttggcg tgcttcacca
     8881 catccgccgc tgcttcaatc cattcaaggt cgctatgtgc gccgaaacca tagttaaagc
     8941 tggaaccctg caaaccgtcg ccatgggcca cttcaatcga atccacgcgg gcatcgtcca
     9001 gtgctttggc aatctggcga acgttttcca gcgaatactg atgacgaatg gcgtgcatac
     9061 cgtcacgcaa cgtgacgtcc gagatataaa gttttttacc gttcatgccg cttctcctgc
     9121 cttgcgcgcc agtgactggg ccattttttc cgctgtcgcc agcgcactgg aagtcataat
     9181 gtcgaggttg cccgcatagg caggcagata atgcgctgcg ccttcgactt ccagccagac
     9241 cgctgttttc agtccggaga attgccccac gcccggtaaa ttgaccggtt tatcctgcgg
     9301 gataacttca aactgcacgc gctgtttcag gcgataaccc ggtacgtaag cctgcaccgc
     9361 ctcagccatt tcattgattg aggcttcgat atcatcttgt gaagcttcgt cgctcaatac
     9421 atacaccgtg tcacgcatca tcaacggtgg ctctgctggg ttaagcacaa taatcgcctt
     9481 cccttttgcc gcgccgccca ccacttcaat ggctcgggaa gtggtttccg taaattcatc
     9541 gatattggca cgcgtgccag gtccggcaga tttactggcg atagaagcga taatttcggc
     9601 gtaatgaaca cgcgccacgc gtgaaactgc cgccaccatt ggaatggtgg cctggccgcc
     9661 gcaggtgacc atgttgacgt tcagttgatc gacgttcgcc tcgaggttaa ccaccggcac
     9721 gcagtaaggg ccgatggcag caggcgtcag gtcaattaag cgaatatccg gtttcgcttc
     9781 gcgtaaagcg gcatcgtttt tcacatgcgc gcccgcgctg gtcgcatcaa atacaatgtc
     9841 gatatcagca aattcaggca tgttcatcag cccgatcacc ccttcatggg tggtggcgac
     9901 gcccatacgt ctggcacgcg ccagaccgtc ggacttagga tcaatgccga ccatcaccgc
     9961 catctccaga tgctgaccgt gacgcaaaat tttaatcatc agatcggtgc caatgttgcc
    10021 agaaccgata atggcgactt tacgcttact catgacagac ttccttttgg ggctgcgctt
    10081 gaaaatgtcg cagcaactga acctatgcct tcaatatggg cttcaaaacg atcgcccgca
    10141 ttcaccgcca ccatcgggcc taatgccccg gtaagaatga tatctccggc gcgcagcggt
    10201 tcacccagac tggccatttt gcgtgccagc cagacggcgg cattaagcgg atgtcccagg
    10261 cattcgctgc cgcgcccgct ggaaacctct tcgttattac gcgtcatctt catggcgcag
    10321 tttttcaggt ctaaccccgc cggacgttgc gccggaccgc cgatgacata caccccgcag
    10381 gaagcgttat ctgccacggt atcgacaaac tgaatcgacc agtcgcgaat gcggctcccc
    10441 accacttcca gcgccggaag tacccattca atggcgttat acagttcgtc gaaggtgata
    10501 tcagttgcgg gtaaatcgcg gttcaacacc agtgcgatct ccgcttcaat gcggggttgc
    10561 agaacacggg aaaaaggaat gatttcgtta tcgccataac acatgtcggc aaataacgtc
    10621 ccaaaatccg gttgatcaac gcccagttgt tgttgcactt tcggatgcgt caggcccact
    10681 ttacgcccta ccacgcgacg cccctgcaca acgtcatatt gcacatttat gtgctgaatg
    10741 gcgtaggccg cttcagcgtt atcgatacca atcagatcgc gcagcggcgc aatcgcttcg
    10801 ccctgctctg cggcgcggcg taaatccgcc gccagttgct caagagtatg cttcgtcatg
    10861 accattcctt aagggcgtgc gaggaaattc agcaccaatt gattgaaagc gtcggcatgt
    10921 tcccactgcg cccagtgacc acagtcgcgg aagatatgca gttcagaacc ggcaatgccg
    10981 gacagcagac gcagacccgc atccatcggc acaaagcggt cgttgcgccc ccagacaatc
    11041 agggtttgcg ctttgatttc cgccagacgt gggccaaaat ccgggaactg tttcggatta
    11101 gcttccaggc tcttaacgaa gttttccagg tgatcgcggc gcgacagcat attattcagg
    11161 cgcgcttcaa acagggcgtc ggtcaaatcg ctggtatcaa aaacgaagat atccatcatc
    11221 agcttcaggt tttcgatagt cggctgacga taaagctgat tcagtcgctt aataccttcg
    11281 gttggcatcg gcgtaaacaa actcatgccg cccgtaccgc cgcccatcag caccagtttg
    11341 ccgacgcgct ccggccattt aagggtgaac gccacagaac tatggccccc catcgagttg
    11401 cccagcaggt ggattttggc gatatccagt tgatccacca cgcttttcag gattcgtgca
    11461 ttaagatccg atcgtgaacc actattaacg atcgaatcgc ttttgcccca acccggacaa
    11521 tccagcagga ttacccgata gcccgcctct accagcggat cgatattgcg gctgaagttc
    11581 gcccagccag tagcacccgg gccggaacca tgcagcagga caacggtttc gtcgccttgt
    11641 ccgcagtcat taaaatggat gcgcagcgtt ttacccgctt cttctacatt cagaaaacgg
    11701 ctggtggcgg cttcggtttg tggctgataa ctcatcatct tctcctgcat attcagttct
    11761 ctgttctggc gcttaacgag ccaaatccgg caatccactc cgggattggg cggtaataac
    11821 gcccttcgct acgccagttg ccaaatgtag aaatagcggc aaaagcggcg acccaggttt
    11881 tgatttcatg tgtcgacttt ccggcaatgg cggaaagctc ttcgttactg acggcatcca
    11941 gttcctgtat gcgtccctgc tccagcaaag tcatgaactg gttatcccaa atcgggttga
    12001 gcggatgcag agttctctga tcctcaacaa acttctcagc ggcgctaatc acccgttgct
    12061 gacgcaattc gcgctcactg gcgggtaaat ctttcccgct ccccaacaga cggtcgcgca
    12121 tatgggcatc ggctttcgcc agttcgggca ccggcggctg atgggaaagc ccaccggaac
    12181 ccaggaacag cacgcgttta ttgagagtgc tggtgaaacg tccaatggct tcacccaaca
    12241 tgcgggtacg ctggaaaccg ggcagcggcg tggcgacacc gttgatgaac acaggcagaa
    12301 ctggcacctt atccagccca ccgagcagga actccagcgg ctgggcgaac ccgtggtcca
    12361 cctgcataca gtaagaaacg gcaagatcga tcccgctctt catgacagca tgcgcacagg
    12421 cctccgccag ctccacaggc acgggcagct ctcctgccgc actgccgaaa tcaccaattg
    12481 ccgtcgctcc aacgcctaaa cagaacggtg gcatcacgtc atagaaaaag ccgttgtagt
    12541 gatctggcgc aaacagcacc accagttcag gggagaaagc cgcaatacgc tcgcgggcgc
    12601 tggcaatcac gccattgacc tcatcgagta cctcttgcgc cgggtcgaca taccccacca
    12661 acggcgagtg ggaaagacag tgaagataag cgtgcatatc aggctacctt ttcgacagaa
    12721 acgtcggcat cagggcgggt cagcgtcatc accgacgcca gtttattcag cgtattgccc
    12781 agggtttgcg gaatggcggt ggcggcaaca aagcgatccg ggcgcatcac caccagcgaa
    12841 gcattgtgtt gcgcgaacca gctacgcaga cgaccttgcg tatcgcccac gcgtagcacg
    12901 ccgtcgtggt tatcctgtgc ggtatgaatt tgcacttccg gcaccacctg aatgaagcgt
    12961 gtgcccaacg cgcgccactg ctggatttgc tcgtcgctca tcccccacag tggattgcat
    13021 ccccagccaa ttaccgcgaa gttcgcgcca atcgcgttat cgagcagcgt cacgtcgccg
    13081 ttttccagcg tgactttcgg ctgaataaac atcttgccga ccggagagtg cttcgcttcg
    13141 ccctcacgta ccagcgcacc gccgtaatat tgcggcatcg gcttgaagcg catttcgagg
    13201 aagtagcgtt ttactggcgg cagataattc agtagccagg aaacgccgtc acgtaacgta
    13261 ccctgccagc gtttcggcgg agccagcacg ttgcccgtcg tcacggacag gtcaatcatc
    13321 gctttggcgt gatcgcgacg ttcttgttga taggtatcga gcagcgcatc gcgggctttc
    13381 ccctggataa ccaacgccag tttccatgcg aggttaaagg cgtcgcgcat accactgtta
    13441 tagccctgcc cctgccatac cggcatgatg tgcgcggcat cgcccgccag cagtacgcga
    13501 tcaatacgga aacgttgcgc cagtcgcgcg ttgtgggtgt agacacgctg gcgaatcaat
    13561 tcaacattgt ccggattagg cagcactttg cttaacagct tgcgcatatt ttgcggctca
    13621 cgcagctgtt cttcggtttc tcccggcatc accataaatt caaagcgacg taccgcatga
    13681 ggcagcgcgg cagaaacata cgggcgcacc ggatcgcaac ataaatagat atgcggcgta
    13741 cttaacggat cgttggcgat atccaccaca atccactgat ttggcgcagt tttaccttca
    13801 aacggcacat tcagggtgcg acgaacaaaa cttgccccac catcacaggc caccagccac
    13861 tgggctttga ctatttcccg ctgcccttct gccgttttca ggtgcaaggt cacttcgtca
    13921 ttttgctggc tgaaggcctc cagctcgcgg gaaaacaagc agcgcacatt aggaaaacgc
    13981 gacacccctt ccagcatcac cgcatcgacc tgcggctgaa taaaggcgtt acggcgcggc
    14041 cagccaaatt catcggtcat tggctgaata tcagcaaaac agcggccttt cggggtgaga
    14101 aaacgcatcg cgtgccacgg cgtagtgtgc ggcagaacat tctcgaccag gccaaccgac
    14161 tgcatggtgc gcagcgcctc gtcatcaata ccaatcgcac gcgggtagtc gatcaactta
    14221 tcgagtttct ccaccaccag cacgtcaatg cccatctgac cgagatagtt cgccatcatc
    14281 agcccaaccg gaccggcacc agcgatcgcc acctgaacgc tatggttaac agcaggctgg
    14341 atgtcagggt gttgtattgc catttcagta cctcacgact cggacaaaat gccgttgcgc
    14401 gcacagtacg gcgcaactta ttttgttaaa aacatgtaaa tgatttttta ttgtgcgctc
    14461 agtataggaa gggtgttttc ggctacaatc aaaacatgcc cgaatgtgca ccaggtgcac
    14521 aacgttgttt taactataga aatgtcaatt aatatgcaga acaatgagca gacggaatac
    14581 aaaaccgtgc gcggcttaac ccgcggtcta atgttattaa atatgttaaa taaacttgat
    14641 ggcggtgcca gcgtcgggct gctggcggaa ctcagcggcc tgcatcgcac cactgtgcgg
    14701 cgactgctgg agacgctgca ggaagaggga tatgtccgcc gtagcccctc cgatgacagt
    14761 tttcgtctga ccatcaaagt gcggcaatta agcgaaggat ttcgtgacga acagtggatt
    14821 tctgcactgg cggccccgct gctgggcgat ctgttgcgcg aagtggtatg gccaacagat
    14881 gtgtccaccc tggatgttga tgcaatggtg gtacgcgaaa ccacccaccg tttcagccgc
    14941 ttatcctttc accgggcaat ggtcgggcga cgtttaccgc tcctgaaaac cgcctcgggc
    15001 ctgacctggc tggccttttg cccggaacaa gaacgcaagg aattaatcga aatgttagcc
    15061 gcccgccccg gtgatgacta tcaactggca cgagaaccgt taaagctgca agccattctg
    15121 gcgcgcgcgc gcaaagaggg ttacggacag aactaccgcg gctgggatca ggaggagaag
    15181 attgcctcta tcgccgtacc gctgcgcagt gaacaacggg tgattggctg tctgaatctg
    15241 gtgtatatgg cgagcgcaat gaccattgaa caggcagcgg aaaagcatct tccggcgcta
    15301 caacgggtag caaaacagat cgaagaaggg gttgaatcgc aggctattct ggtggccgga
    15361 cggcgaagcg gcgtgcattt acgttgacac catcgaatgg cgcaaaacct ttcgtggtat
    15421 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgct
    15481 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttctcgcg tggtgaacca
    15541 ggccagccac gtttctgcga aaacgcgaga aaaagtggaa gcggcgatgg cggagctgaa
    15601 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt
    15661 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg
    15721 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc
    15781 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
    15841 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt
    15901 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg
    15961 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc
    16021 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac
    16081 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt
    16141 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa
    16201 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc
    16261 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt
    16321 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
    16381 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
    16441 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    16501 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    16561 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    16621 tgttgtgtga aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
    16681 acggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacacaa
    16741 cttaatcgcc ttgcggcaca tccccctttc gccagctggc gtaatagcga agaagcccgc
    16801 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt
    16861 ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga ggccgatact
    16921 gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcccatcta caccaacgtg
    16981 acctatccca ttacggtcaa tccgccgttt gttcccacgg agaatccgac gggttgttac
    17041 tcgctcacat ttaatgttga tgaaagctgg ctacaggaag gccagacgcg aattattttt
    17101 gatggcgtta actcggcgtt tcatctgtgg tgcaacggtc gctgggtcgg ttacggtcag
    17161 gacagtcgtt tgccgtctga atttgacctg agcgcatttt tacgcgccgg agaaaaccgc
    17221 ctcgcggtga tggtgctgcg ctggagtgac ggcagttatc tggaagatca ggatatgtgg
    17281 cggatgagcg gcattttccg tgacgtctcg ttgctgcata aaccgactac acaaatcagc
    17341 gatttccatg ttgccactcg ctttaatgat gatttcagcc gcgctgtact ggaggctgaa
    17401 gttcagatgt gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt atggcagggt
    17461 gaaacgcagg tcgccagcgg caccgcgcct ttcggcggtg aaattatcga tgagcgtggt
    17521 agttatgccg atcgcgtcac actacgtctg aacgtcgaaa acccgaaact gtggagcgcc
    17581 gaaatcccga atctctatcg tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt
    17641 gaagcagaag cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg
    17701 ctgaacggca agccgttgct gattcgaggc gttaaccgtc acgagcatca tcctctgcat
    17761 ggtcaggtca tggatgagca gacgatggtg caggatatcc tgctgatgaa gcagaacaac
    17821 tttaacgccg tgcgctgttc gcattatccg aaccatccgc tgtggtacac gctgtgcgac
    17881 cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa cccacggcat ggtgccaatg
    17941 aatcgtctga ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg
    18001 gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt cgctggggaa tgaatcaggc
    18061 cacggcgcta atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg
    18121 gtgcagtatg aaggcggcgg agccgacacc acggccaccg atattatttg cccgatgtac
    18181 gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga aatggtccat caaaaaatgg
    18241 ctttcgctac ctggagagac gcgcccgctg atcctttgcg aatacgccca cgcgatgggt
    18301 aacagtcttg gcggtttcgc taaatactgg caggcgtttc gtcagtatcc ccgtttacag
    18361 ggcggcttcg tctgggactg ggtggatcag tcgctgatta aatatgatga aaacggcaac
    18421 ccgtggtcgg cttacggcgg tgattttggc gatacgccga acgatcgcca gttctgtatg
    18481 aacggtctgg tctttgccga ccgcacgccg catccagcgc tgacggaagc aaaacaccag
    18541 cagcagtttt tccagttccg tttatccggg caaaccatcg aagtgaccag cgaatacctg
    18601 ttccgtcata gcgataacga gctcctgcac tggatggtgg cgctggatgg taagccgctg
    18661 gcaagcggtg aagtgcctct ggatgtcgct ccacaaggta aacagttgat tgaactgcct
    18721 gaactaccgc agccggagag cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg
    18781 aacgcgaccg catggtcaga agccgggcac atcagcgcct ggcagcagtg gcgtctggcg
    18841 gaaaacctca gtgtgacgct cccctccgcg tcccatatca tcccgcaact aaccaccagc
    18901 gaaacggatt tttgcatcga gctgggtaat aagcgttggc aatttaaccg tcagtcaggc
    18961 cttctttcac agatgtggat tggcgatgaa aaacaactgc tgacgccgct gcgcgatcag
    19021 ttcacccgtg caccgctgga taacgacatt ggcgtaagtg aagcgacccg cattgaccct
    19081 aacgcctggg tcgaacgctg gaaggcggcg ggccattacc aggccgaagc agcgttgttg
    19141 cagtgctcgg cagatacact tgccgacgcg gtgctgatta cgaccgctca cgcgtggcag
    19201 catcagggaa aaaccttatt tatcagccgg aaaacctacc ggattgatgg tagtggtcaa
    19261 atggcgatta ccgttgatgt tgaagtagcg agcgatacac cgcatccggc acggattggc
    19321 ctgacctgcc agctggcgca ggtcgcagag cgggtaaact ggctcggatt agggccgcaa
    19381 gaaaactatc ccgaccgcct tactgcggcc tgttttgacc gctgggatct gccattgtca
    19441 gacatgtata ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa
    19501 ttgaattatg gcccacacca gtggcgcggc gacttccagt tcaacatcag ccgctacagt
    19561 caacagcaac tgatggaaac cagccatcgc catctgctgc acgcggaaga aggcacatgg
    19621 ctgaatatcg acggtttcca tatggggatt ggtggcgacg actcctggag cccgtcagtg
    19681 tcggcggaat tccagctgag cgccggtcgc taccattacc agttggtctg gtgtcaaaaa
    19741 taataataac cgggcaggcc atgtctgccc gtatttcgcg taaggaaatc cattatgtac
    19801 tatttaaaaa acacaaactt ttggatgttc ggtttattct ttttctttta cttttttatc
    19861 atgggagcct acttcccgtt tttcccgatt tggctacatg acatcaacca tatcagcaaa
    19921 agtgatacgg gtattatttt tgccgctatt tctctgttct cgctattatt ccaaccgctg
    19981 tttggtctgc tttctgacaa actcgggctg cgcaaatacc tgctgtggat tattaccggc
    20041 atgttagtga tgtttgcgcc gttctttatt tttatcttcg ggccactgtt acaatacaac
    20101 attttagtag gatcgattgt tggtggtatt tatctaggct tttgttttaa cgccggtgcg
    20161 ccagcagtag aggcatttat tgagaaagtc agccgtcgca gtaatttcga atttggtcgc
    20221 gcgcggatgt ttggctgtgt tggctgggcg ctgtgtgcct cgattgtcgg catcatgttc
    20281 accgtcaata atcagtttgt tttctggctg ggctctggct gtgcactcat cctcgccgtt
    20341 ttactctttt tcgccaaaac ggatgcgccc tcttctgcca cggttgccaa tgcggtaggt
    20401 gccaaccatt cggcatttag ccttaagctg gcgctggaac tgttcagaca gccaaaactg
    20461 tggtttttgt cactgtatgt tattggcgtt tcctgcacct acgatgtttt tgaccaacag
    20521 tttgctaatt tctttacttc gttctttgct accggtgaac agggtacgcg ggtatttggc
    20581 tacgtaacga caatgggcga attacttaac gcctcgatta tgttctttgc gccactgatc
    20641 attaatcgca tcggtgggaa aaatgccctg ctgctggctg gcactattat gtctgtacgt
    20701 attattggct catcgttcgc cacctcagcg ctggaagtag ttattctgaa aacgctgcat
    20761 atgtttgaag taccgttcct gctggtgggc tgctttaaat atattaccag ccagtttgaa
    20821 gtgcgttttt cagcgacgat ttatctggtc tgtttctgct tctttaagca actggcgatg
    20881 atttttatgt ctgtactggc gggcaatatg tatgaaagca tcggtttcca gggcgcttat
    20941 ctggtgctgg gtctggtggc gctgggcttc accttaattt ccgtgttcac gcttagcggc
    21001 cccggcccgc tttctctact gcgtcgtcag gtgaatgaag tcgcttaagc aatcaatgtc
    21061 ggatgcggcg cgagcgcctt atccgaccaa catatcagga cggaatgatc gcaatgaaca
    21121 tgtcgatgac agaaagaata aaagcaggca agctatttac cgatatgtgc gaaggcttac
    21181 ctgaaaaaag acttcgtggg aaaacgttaa tgtatgagtt taatcactcg catccatcag
    21241 aagttgaaaa aagggaaagc ctgcttaaag aaatgtttgc cacggtaggg gaaaacgcct
    21301 gggtagaacc acctgtctat ttctcttacg gttccaacat ccatataggc cgcaattttt
    21361 atgcaaattt caatttaacc attgtcgatg actacacggt aacaatcggt gataacgtac
    21421 tgattgcacc caacgttact ctttccgtta cgggacaccc tgtacaccat gaattgagaa
    21481 aaaacggcga gatgtactct tttccgataa cgattggcaa taacgtctgg atcggaagtc
    21541 atgtggttat taatccaggc gtcaccatcg gggataattc tgttattggc gcgggtagta
    21601 tcgtcacaaa agacattcca ccaaacgtcg tggcggctgg cgttccttgt cgggttattc
    21661 gcgaaataaa cgaccgggat aagcaatatt atttcaaaga ttataaagtt gaatcgtcag
    21721 tttaaattat aaaaattgcc tgatacgctg cgcttatcag gcctacaagt tcagcgatct
    21781 acattagccg tatccggcat gaacaaagcg caggaacaag cgtcgcatca tgcctctttg
    21841 acccacagct gcggaaaacg tgctggtgca aaacgcaggg ttataatcat cagcccaacg
    21901 acgcacagcg catgaaatgc ccagtccatc aggtaattgc cgctgatact acgcagcacg
    21961 ccagaaaacc acggggcaag cccggcgatg ataaaaccga ttccctgcat aaacgccacc
    22021 agcttgccag caatagccgg ttgcacagag tgatcgagcg ccagcagcaa acagagcgga
    22081 aacgcgccgc ccagacctaa cccacacacc atcgcccaca agaccggcaa ttgcagcggc
    22141 aaccagataa agccgcagaa ccccaccagt tgtaacacca gcgccagcat taacagtttg
    22201 cgccgatcct gatggcgagc catagcaggc atcagcaaag ctcctgcggc ttgcccaagc
    22261 gtcatcaatg ccagcaagga accgctgtac tgcgcgctgg caccaatctc aatatagaag
    22321 gcgggtaacc aggcaatcag gctggcgtaa ccaccgttaa tcagaccaaa gtaaacaccc
    22381 agcgtccacg cgcgaggagt gaaaaccacg cgaaccggag tggttgttgt cttgtgggaa
    22441 gaggcgacct cgcgggtgct ttgccaccac caggcaaaga gcgcaacaac ggcaggcagc
    22501 gcccaccagg cgagtgtttg ataccaggtt tcgctatgtt gaactaacca gggtgtcagg
    22561 gcggcaccaa gcccaccgcc gcccatcaga gccgcggacc atagccccat caccagtggc
    22621 gtgcgctgct gaaaccgccg tttaatcacc gaaggcatca ccgcctggat gatgccgatc
    22681 cccaccccac cgagcagtgc gctgctaagc agcagcgcac tttgggggta aagctcacgc
    22741 atcaatgcac cgacggcaat cagcaacaaa ctgatggcga cgctgcgacg ttcgctgaca
    22801 tgctgatgaa gccagcttcc ggccagcgcc agcccgccca tggtaaccac cggcagagcg
    22861 gtcaacaggg cagccacgct aaagctcatt ccgctcgcct ggcgcaattg cggtagcagt
    22921 ggcccgacgg aggtgagcag tggtcgcata ttaagaccaa tcagcaccag aaccagcagt
    22981 atcgtgaacg tcttttgatg gttgcgcatg gctgtcagaa cggtttggtc ggcagatatt
    23041 taccatctaa ggtgatgacc gcacgttcgc caccttccgg gtccgccact ttcttaacgt
    23101 cgagtttgaa gttaatcgcg ctgataatgc catcgccaaa cttctcatga accaacgctt
    23161 tcagggttgt gccgtacacc tgcaacattt catagaaacg atacatcgtt gggtcggttg
    23221 gaatacggtc atcaatgcag ccacgcagtg gaatcatctg caacagtaga atggcgtctt
    23281 cgtcgagatc cagcttcgct ccgaccaggc gggcggcgtc ggcaggaagc gcctgctgcc
    23341 ccagcaaagc cgcggttacg aaggcttctg ccagaccggt accgtcggca atctcggcaa
    23401 atgagagatc ttttttagct ttgctgagca aaatggcatc ggcaagatca agacgaatat
    23461 tgcggttaat ttgcgactga atcatggtgc atctcctgat ggtttaaaaa taaggttacg
    23521 ctgcggtcgg ttggcgtagc ggtatggcac aaacgcgcgg attagctgcc agtggcacaa
    23581 actggcgggt tgcgccgtca aacgctgcga tgctgccgct ttcaatgtcg tagacccagc
    23641 cgtgcagggc gatccgcccc tcttcgagcg ccaggcgcac cgatggatga gtttgcaaat
    23701 tagccaactg agcaatgacg ttttcacgta ccatcgccgc agcttttgac ggtaaatcgg
    23761 aatgcgggcg cgcctcatta acgacgcggg ctgaatcggc ataacgcagc cagtgggaga
    23821 cggcaggcat atggtccatg cactgacagc tggcaatggc ggtcatcgcg ccacagttgg
    23881 aatgaccgca aatcacaatg tcagataccc gaagcgcagc gacggcatac tccaccgaag
    23941 cagaaacgcc accgggttcc ggcccgtagg aagggacgat attgcccgcg ttgcgaataa
    24001 cgaacagatc gccaggctca cgttgcgtca ccagctcagg gaccagacgg ctgtcggagc
    24061 aggagataaa aagtgtgcgc gggctttgct gtgtcgccag ctgtttaaac aaggcttccc
    24121 gcttcggaaa tgcctcgcgc tggaatttaa ggaatccatc aataatctct ttcaccgtta
    24181 acctctgtct gtctctggaa tgagaggcag actactcgcc gattgtcata aggtaaaagt
    24241 ctcatttatg atgagttcta ttggatttac ttataggttg gcgaatgctc tctcgacata
    24301 tcaattattt tcttgccgtg gctgaacatg gcagcttcac ccgtgccgcc agtgcgttgc
    24361 acgtctctca gcctgcgctt tcccagcaga ttcgccagtt agaggagagt ttaggcgtgc
    24421 cgctgtttga ccgtagcggg cgaacgattc gtctcaccga tgcaggagaa gtctggcgac
    24481 agtacgccag ccgggcgtta caggaactgg gagcgggtaa acgggcgatt catgatgttg
    24541 ccgatctgac gcgaggatcg ctgcgtatcg ccgtcacccc cacctttaca agctacttta
    24601 tcggcccttt aatggcggat ttctatgcgc gctaccccgg catcacgctc cagctacagg
    24661 aaatgtcgca ggagaaaatc gaagatctgc tttgtcgcga cgagctggac gttgggattg
    24721 acttcgcgcc tgtgcattcg ccggagctgg aggcaattcc tttactgaca gaaagtttag
    24781 cgttagtcgt ggcgcaacat catccgctgg ctgcctgtga acaggtggcg ttgagtcgct
    24841 tgcatgatga aaaactggtc ctgctcagcg cggaatttgc caccagagag caaattgacc
    24901 actactgcga gaaagcgggg ctacatccac aggtggtcat tgaggcgaac tcaattagcg
    24961 cggttctgga gctgattcgc cgcacttccc tttccacatt gttaccagca gcgattgcca
    25021 cacaacatga cgggcttaaa gcgatttctc ttgccccgcc actactggag agaacggcgg
    25081 ttttgttgcg gcggaaaaat agctggcaga cagccgccgc gaaggcattt ttgcacatgg
    25141 cgttggagga atgtgcggat gttggcgaga atgaatcacg gtagccactg acacaggtca
    25201 gtggctcttc aacacagagg aaattaccgt ttgtaatcga tggcttctgg ctgctccaga
    25261 tatacggtgg tttgtgccgg ttgtgtactg gcaatcacct tgccgccacg taccgaataa
    25321 cgtaccggaa cctgacggcg cagcgcatca aatccatttt cagccggcag gataatcagg
    25381 tttgcgctgt ttccggcggc aatgccgtaa tcctgcaaat tcaatgttct ggcactgtgg
    25441 tgggtgatta aattcaggcc atcgttaatc tgcccgtagc ccatcaactg gcaaacatgc
    25501 agccccatat gcagcacttg cagcatattc gccgttccca gcggatacca cggatcgaag
    25561 acatcatcgt gaccaaagca gacgttaatg ccggactcca gcatctcttt aacgcgcgtg
    25621 atgccgcgac gttttggata cgtatcgaaa cgtccttgca gatgaatatt gaccagcggg
    25681 ttggcgacaa agttaatacc ggacattttc agcaagcgga acaggcgtga ggtatacgcc
    25741 ccgttatagg agtgcattgc cgtggtgtgg ctggcggtga ctcgcgcgcc catgccttca
    25801 cggtgcgcca gggcagcaac ggtttcgaca aagcgcgact gctcgtcatc gatctcatca
    25861 cagtgaacgt cgatgagacg gtcgtatttt tgcgccaggg cgaaggtttt atgcagcgac
    25921 tccacgccgt attcacgggt aaattcaaaa tgcggaatcg cccccactac atctgcccct
    25981 aagcgtaacg cctcttccag caacgcttca ccgttgggat acgacaaaat cccttcctga
    26041 gggaaggcga caatttgcag atcaatccac ggcgcgactt cctgcttcac ttccagcatt
    26101 gctttcagcg cagttagcgt tgcatccgaa acatcgacat gggtacgcac atgctgaatg
    26161 ccgttggcaa tctgccattt cagcgtttgc caggcgcgtt gtttcacatc gtcatgggtt
    26221 aataacgctt tgcgctcggc ccagcgttca atgccttcaa acagcgtgcc ggactgattc
    26281 cagttcggtt gtccggcggt ttgcgtggtg tccaggtgaa tatgcggctc cacaaacggc
    26341 ggtataacta aaccttgttc ggcatccagg ctgttttcag ttatgggcat cacgccggat
    26401 tgcgcatcaa tggcgctgat ttttccgtcc tgcagatgaa tctgccacag cccctctttg
    26461 cctggtaacc gggcgttaat aattgtttgt aaagcgttat tcgacactgt taacctccac
    26521 atgcgtcatt gctgctgtcg ttttacgatt caaaatcggg ttaaggatca gatagctcag
    26581 cgcgccacct aataccgcgt tgaccggaac aattcccggt aaccagtggc ctgcggcaat
    26641 ccccagcgcg accgccagaa tcgccaccca attgacactc atcatacgcg tggtcgcaaa
    26701 gtgctcatag cggcgacggt tcatcagata gtcggcgatg atcacgcccc ctaccggagg
    26761 aatagctgcc gaaaggaagg tcaaccagcc gacaaaattg ttatacagcc ataatgcgca
    26821 gaccgtaccg ataataccgt tgattaccga aagggttttg ctcgacatac cggtaatgtt
    26881 ggcgaaacct aaacccgacg catagagtgc gttatcgttg gtggtccaga tattcagccc
    26941 cagcaccaca atcgcgggca gcagcagtcc ctgagcaatc atcacatcag agatatctgc
    27001 catccccagt gccgcagcgc ccgctgcacc gaaaataaac atcaacgagt tgccgaggaa
    27061 aaaggccacc atcgccacca gcaccgccag tttggcattg cgaccaaacc ggacaaagtc
    27121 agcggtgagc gtacccgcac tgataaatga ccccacaacc agcgccagcg cgacattgaa
    27181 atctaacggt tgtgcgggaa cgaccgcttt taaggcgtcc aggccgccca tgccgttaac
    27241 ggccagccac acggaataac cgccaaggca ggcaatagcc ggaaccgcaa tcaccgaaag
    27301 aactgtcagc gccgaaatgc caaagaagac ggtgacggtc atcagtaaac cggaaacggc
    27361 aatcagcaaa ttaatatcca gcccggttgc cttgcccacc ggaatagcaa acatcgccac
    27421 gccaacgcca aaccagccaa cctgagttcc gcccagtagc agtgaaggca gccatgagcc
    27481 tttaacacca aacgagaagc gagcaagaag atgagtggtc aggccggttt ttgcgccaat
    27541 gtaaccgaga aatgaagtgt aaataccgag gagaagatta ccgatgagaa ctgcgaggaa
    27601 gaaatcatga tagctaagac cggttccgag agtgccgccg gtccacatac tggcggaaaa
    27661 gaaggttaat cccagcatga cgaacgtcaa tgccaatacc cctttccgcg ccgactgcgg
    27721 gactggcccc tggctaaagt tgttatcttg cgacacgaaa ttcccccttt ttgctgtttg
    27781 aaacccaaaa aatccgccgc attctattca tctgaagata aaaagcaatc gttttcgtgg
    27841 ggaaatatat tttttatatg aatgagtgca aaaaggtggg ggaaaaatta tcggtgtaaa
    27901 aacagtgggt aggcataata agacgcggta agcgtcgcat caggcatcgg cgcggggtgt
    27961 cggatgcgac gcgagcgcct tatccgacct acggttcagg cctgtaggca taataagacg
    28021 cggtaagcgt cgcatcaggc atcggcgcat ggtgtcggat gcgccacgcg acgcatccga
    28081 caatacctta ctcttccatc gcctggcgga tctgatccaa tgacgcagga tcatcaatgg
    28141 tcgtcagatc cccaggatcg cgtccttcgc aaattgcctg gatcgtgcgg cgcagcattt
    28201 ttccggatcg cgttttcggc aattgcgaga caaaccagac gtgcgccggg cgaccaaagt
    28261 tgccaatctg gctgtccacc agcgccataa tcgccttctc ttgcgaatgc gccacatcac
    28321 gatcttccag actatcgctc tctttcggaa tgacaaacgc caccgccacc tgccctttca
    28381 gcgcatcttt caccccaacc accgccactt cggcaacgcc cggatggctg gagatactct
    28441 cttcaatctc gcgcgtgccc agacgatgcc cggcaacgtt aattacatcg tcagtgcgcc
    28501 cgagaataaa gtgataaccg tcagcgtcac ggatgcccca gtcaaaggtg gcgtacaccg
    28561 ggcgggaaaa cagcgaccag taagtcttca caaagcggtc gtcgtcgccc cagatggtct
    28621 gaatacagcc cggcggcagc ggcccttcca ccaccagcat ccctttctcg ttgacgccac
    28681 acggttcgcc ggtgacttca ttaagcaact gcacgttata gccatacatc ggcacgccgg
    28741 ggcttcccag acgcgtcggc ctgtcgtcca gaccgcgagc aatcgccata atcggccagc
    28801 cggattcggt ctgccagtag ttgtcgatga ccggcacatc cagcgtattg ctcacccaac
    28861 tggcggtcgg ctcgtccagc ggttctccag ccagatagag cacttccagc gacgagagat
    28921 cgtgtttgcg aatttcagcg gtagggaatt ttttcagcac gcgaatggcg gtcggcgctg
    28981 agaacatccg gctaacctga tatttctcga cgattttcca ccacacgccg cagtccggcc
    29041 aggtcggcaa tccttcgtaa acgatagtcg ccatccccgc cagcagcggc gcgtaaacga
    29101 tatacgaatg ccccaccacc cagccgatat ccgatgcgca aaagaacacg ctgcccgctt
    29161 tgccgccaaa aatggtgtcc atcgaggtcg ccagcgccac cgcatatccg ccgacgtcac
    29221 gctgcacgcc tttaggtttg ccggtcgtgc cggaagtgta gagaatgcag gaggtttcgt
    29281 tggattccag ccacgccacc ggtacccgcg cgccgatgtg ttgatggcgc aacgacgcga
    29341 aatcgacatc ccgcccgctg acgcgcgcca ttttcgccag cccgcgatcc accagcaaaa
    29401 catggcgtgg ctggtgctgc gcctgactta tcgcatcgtc gagcaatttt ttatagggaa
    29461 tgattttgcc accgcgcgct ccggcatcag ccgagacaat cagcaccggt ttagcgtcat
    29521 caattcgcgc cgccacgctg tgcgaggcaa aaccaccaaa taccaccgaa tgaatagcac
    29581 caatgcgcgc gcaggccagc agagtaatat gcgcttcggc aatcatcggc atatacacca
    29641 gcacccgatc gccgcgctgc acgcccagtg agcgcaacat tgaggccacc gcgttcactt
    29701 cgtcatgcag ctggcgaaag gtaaaggtgc gctcttcttc tgtttccgaa gagacggcaa
    29761 tcaatgccag tgcctctggc tgtttctcca gccagcggtc gatagcgttg tggcacaaat
    29821 tggttcggcc ttcacaaaac caacgggcaa acggcggatt gctgtggtcg agcgtttgcg
    29881 taaagggcgt ctgccagtca atacgccggg cctgctcggc ccagaactgc tccggttcgt
    29941 taatcgaacg ctgataaaat tcgctaaaag acataatgct ctcctgttga acttacgcct
    30001 taccgccgtt tacttaaatg acgtacaggt cgagatactc attgaccggc atctgttcca
    30061 ggcgagttct gtcgagagaa acctccagaa tgcgctgctg ttggcgagtc gggaactggc
    30121 gcgcgagatt gattttgaat ttatcgacca gtttcggaat accatcctga cggcggcgag
    30181 catgaccaat ggggtactcc accaccactt cttcaaatcg tgtgccgtcg gtgaactcaa
    30241 gggtaatggc attggcgatg gcgcgttttt ccgggtcgtg gtagtcagcg gtaaatgccg
    30301 gatcttcaaa gcaattgatc ttctcgcgca gggcgtcgat gcgtttatct tgcgcaacgt
    30361 tgtcctcgta atctgccgcc gttaagcgcc cgaataacag cgggatcgcc accatgtact
    30421 gaatgcagtg gtcgcggtca gccgggttat tgagcggccc ctttttgtcg atgatgcgaa
    30481 tacaggcttc gtgggtgcga atggtcactt tttcgatatc cgccgccgtc ttgcctgctg
    30541 cctgcatctg ttcatagagc gtcatcgccg cttcaactgc cgtctgggag tggaactccg
    30601 ccgggaagga gattttgaac agcacattct ccatgacgta agaaccgtac ggacgctgga
    30661 agcggaacga ttcaccttta aaggagacgt cgtagaagcc ccataccggc gcggtcaggg
    30721 ctgacgggta acccatttcg cccgttttcg ccatcagtgc cagacgtacc gcgcgggaag
    30781 tggcatcgcc cgccgcccag gatttacgcg tgccggtgtt cggcgcatgg cgataggtgc
    30841 gcagcgactg accgtccacc cacgccagcg aaacggcgtt gagaatttcc tcgcgggtca
    30901 ggccgagcat ttcggcgacc acggcggtgg aagccacttt cactaacaga acgtggtcga
    30961 ggccgacgcg gttaaaggag ttttccagcg cgatgcagcc ctgaatttca tgggctttga
    31021 tcattgcggt cagcacctgt ttcatggtca acggcgcttt gccgctggcg accgcgttgc
    31081 gcgaaagcca gtctgccgtt gccagaatgc cgccgaggtt gtcggaagga tgcccccact
    31141 ccgccgccag ccaggtatcg ttgaaatcga gccagcggat catcgcgcca atgttaaatg
    31201 ccgcctggac ggggtcgagc tgaaactgag ttccggggac gcgcacgccg ttgggtacga
    31261 cggtgccagg aacaattggc cccagcagtt ttttacaggc cgggtattcg agagcttcaa
    31321 gaccgcagcc gagcgtgtcg agcaggcagt aatgtgcggt gtcgtaggcc actttagagc
    31381 tgatttcgta gttcatcacg taatcgacga tatcaacgat ttcacgatca aattccgggc
    31441 ggatgttgtt gatttgagct gacattgggt acgtttcctt attgttattc gtagaggttt
    31501 actggcgctt atccagcgcg acaaactggc ggtcttccgg tccaacataa ttggcggaag
    31561 gacggataat tttgttgtcc tgacgttgtt caatgatgtg cgccgcccag ccggtgacac
    31621 gggcgatgac aaacagtggt gtgaacatct cggtgggaac gcccatcatg ttgtaggaaa
    31681 cagcagagaa ccagtcgaga ttggggaaca tctttttgct ctcccacatc accgtttcca
    31741 ggcgatcggc gatgttgtac atcttcaacg agccgccttc ctgcgagagc tgcttcgcca
    31801 cacgtttgat cacctggtgg cgcgggtcgg cgatggtata aaccggatga ccaaaaccaa
    31861 tgaccacttc tttgttttcc acccgcttgc ggatatcggc ttcggcttcg tccggcgttt
    31921 cgtagcgttg ctggatctcc agcgacactt cattcgctcc gccgtgtttc ggcccgcgca
    31981 gtgcgccaat cgcgccaata atcgcggaat acatatcaga gccagtgccc gcaatcaccc
    32041 gactggtaaa ggtggaggcg ttaaactcgt gttcggcgta cagcactagc gaaatatgca
    32101 tcgccttttc ccagctttgc gatggctttt cgccgtgcag cagatgcagg aagtgaccac
    32161 cgatggagtc gtcatcggtt tccggctgga tgcgttcgcc gttgtgactg tagtgatacc
    32221 aataaaggag aatcgagcta agtgatgcca gtagtttgtc ggcaatatcc cgcgcgccag
    32281 agacggtatg tccctctttt tctggcagcg tgcagccgag cgcggaaacg ccggtgcgca
    32341 taacatccat cgggtgtgac gccgccggta aggcttccag cacggtacgc acgttagccg
    32401 gtaaaccgcg cagggctttc agtttcgttt tgtaagcggc gagttcgtca cgggtcggca
    32461 gtttgccgtg gatcagcaga tgcgccactt cttcaaattc gcaatgttcc gccagatcaa
    32521 gaatatcgta gccgcggtaa tgcaggtcat tgccgctttt acccacggtg cagagcgccg
    32581 tattgcccgc cggaacgcca gaaagtgcca cagatttttt cggtttaatg acatgggtac
    32641 tgttttgcag gatcgttgtg tcgctcatgt tgtcctcgtc atttgtaggg tcgagatatt
    32701 tttcgttgtg ttttatttca cctggctacg ggcaaacagg tcgtcgagct tcgcttcgta
    32761 ctggtagtag ttgatgcttt cgtacagctc gttgcgggtt tgcatggtat cgatgacgct
    32821 tttctgcgtg ccttcctggc gcaggacgtt gtagacatgc tcagcggcgc ggttcatggc
    32881 gcggaacgct gaaagtgggt acagcgccat tgcgacatgg gcgctgcgta attcgtcggt
    32941 ggtaaacagc ggtgttgcgc caaattcggt aatgttggcg aggatcggca cctgcaccgc
    33001 atcggcaaac tggcggtaca tggcgagttc ggtaatcgcc tccgggaaca acatctcggc
    33061 acccgcttca acataggcct gtgcacgctc gatcgccgca tccagcccct ctaccgccag
    33121 cgcatcggtg cgcgccatga tcacaaaatc aggatcggtt ttcgcatcca ccgccgcgcg
    33181 gatccgatcc accatctcct ctttcgagac gatcgctttg ttcgggcgat gaccgcagcg
    33241 tttcgcacca acctgatctt caatatgcaa tcctgccgca ccggctttaa tcatcgattt
    33301 caccgtacgc gccacgttaa aggccgaaga accaaaaccg atatccgcat ccaccagcag
    33361 tggcagcgaa caaacgtcgg tgatacggcg aatatctgtc agcacatcat caagggtaga
    33421 aataccgaga tcgggcagcc ccagcgaccc tgccgccacg ccaccgccgg agagataaat
    33481 tgcctgatat ccggcacgct gcgccaacag cgcatgatta gcgttgatgg tgccaacaat
    33541 ctgcaatgga ttttctttag tcagtgcagc gcgaaacgct ttacctggag agtgtagaga
    33601 catagcccat cctttgttat caacttgtta ttttcgttaa taaaacaaag caaggggtgt
    33661 gccacaattg ccttaataaa attaaatgtt taatttcaaa ttgttagaga taacgtcatg
    33721 aactaatccg caaatatgcg tttcagttaa cgtttcaggc aatgtttcac cgcgtttcat
    33781 tgcaacaatt atgaaacaag actaaaccca atattcggtt tcttaacttt gcggtgcgct
    33841 atggcacatc caccacggct taatgacgac aaaccggtta tctggacggt atctgtaacg
    33901 cgcctgttcg agctgtttcg cgatatcagc ctcgagtttg atcacctggc gaacattacc
    33961 cctattcagc ttggctttga aaaagcagtg acctacatcc gcaagaaact ggcaaacgaa
    34021 cgctgtgacg ccatcatcgc tgctggctct aacggcgcgt acctgaaaag ccgcctgtca
    34081 gtgccagtta ttttgattaa accgagcggc tacgatgtgt tacaggcact ggcaaaagcc
    34141 ggaaaactca cctcttctat cggcgttgtc acctatcagg aaaccattcc agcgctggtg
    34201 gcgtttcaaa aaacctttaa tctgcgcctc gatcaacgta gctacattac cgaagaagac
    34261 gcccgcgggc agattaacga gctgaaagct aacggcactg aagcggtggt cggcgcgggg
    34321 ctgattaccg atctggcaga agaagccgga atgaccggaa tttttatcta ttccgccgcc
    34381 accgtgcgcc aggcgttcag cgatgcgctg gatatgacgc gcatgtcgtt acgccataac
    34441 actcacgatg ccacccgcaa cgccctgaga actcgttacg tgctgggcga tatgctcggt
    34501 caatcaccac agatggaaca agtacggcag actattttgc tgtatgcccg ctccagtgca
    34561 gcggtgttga ttgaggggga aacggggacg ggcaaagagc tggcggccca ggcgattcat
    34621 cgggaatatt ttgcccgcca cgatgcgcga cagggcaaaa agtcgcatcc gtttgttgcc
    34681 gtcaactgcg gggcgatagc cgaatcgctg ctggaagcgg agctgtttgg ctatgaggaa
    34741 ggggcgttta ccggctcgcg acgcggaggt cgcgccgggc tgttcgaaat tgcccacggc
    34801 ggtacgctgt ttctggatga gattggcgaa atgccgctac ctttgcagac tcgcctgtta
    34861 cgggtgctgg aagaaaaaga ggtcacccgc gtcggcgggc atcagcctgt tccggtagat
    34921 gtacgggtca ttagcgccac tcactgcaag ctggaagaag atatgcagca aggacgtttt
    34981 cgccgtgatc tgttttatcg gctgagtatt ttgcgtctgc aattgccacc actgcgcgag
    35041 cgggtggcgg atattcttcc gctggcggaa agctttttga aagtgtctct ggctgcgctc
    35101 tccgccccgt tttctgccgc attacgccag ggattacagg caagcgaaac cgtgctggtg
    35161 cactacgact ggccaggtaa tattcgtgaa ctgcgcaata tgatggagcg actggcgcta
    35221 tttttaagtg tggaaccgac gccggattta acgccgcaat ttttgcagct gctactgccg
    35281 gaactggcgc gcgagtcggc gaaaactccc gctccacgct tactgacacc acaacaggca
    35341 ctggagaaat ttaatggcga taaaacagca gcggcgaatt atttaggcat cagccggacg
    35401 acgttctggc ggcggctgaa aagctgaact gccgaatgca gcgcgaacgc tttatccggc
    35461 ataaatttca atagatgatc accgataaac ttacgcatcg ggcatgttta catagggttc
    35521 agaattactt cttcttataa atatttgccg tgccgtggat cttattgtca gtttgaccgg
    35581 aggtcagtac caacacatca gccccttttt catccgcttt tttgatcaaa tcttcttttg
    35641 catctgcagt cgacatttca ttgctggttg aaatatcacc tattttttca tactgcgatt
    35701 caactttttc aaattccgct ttggtcatca attcagcggc atagacattg gtaacggcta
    35761 acgctaacgc accgactaac attttagaga ttattttcat attacggctc cagagttgtt
    35821 atcacactat catccaccca cattattggg ttgattaata attagttcag tcagggcata
    35881 aacgccagta accggaaata agacactttg tgatctctct cgcacccgat tatttttggt
    35941 agaaatatga tgaagttact tcacttattt atggatgaaa tcactatgga tcctttgcac
    36001 gccgtttacc tgaccgtagg actgttcgtg attacttttt ttaatccggg agccaatctc
    36061 tttgtggtag tacaaaccag cctggcttcc ggtcgacgcg caggggtgct gaccgggctg
    36121 ggcgtggcgc tgggcgatgc attttattcc gggttgggtt tgtttggtct tgcaacgcta
    36181 attacgcagt gtgaggagat tttttcgctt atcagaatcg tcggcggcgc ttatctctta
    36241 tggtttgcgt ggtgcagcat gcgccgccag tcaacaccgc aaatgagcac actacaacaa
    36301 ccgattagcg ccccctggta tgtctttttt cgccgcggat taattaccga tctctctaac
    36361 ccgcaaaccg ttttattttt tatcagtatt ttctcagtaa cattaaatgc cgaaacacca
    36421 acatgggcac gtttaatggc ctgggcgggg attgtgctcg catcaattat ctggcgagtt
    36481 tttcttagtc aggcgttttc tttgcccgct gtgcgtcgtg cttatgggcg tatgcaacgc
    36541 gttgccagtc gggttattgg tgcaattatt ggtgtattcg cgctacgcct gatttacgaa
    36601 ggggtgacgc agcggtgagc agataattta ctctctacgt aatcaacctg atttgccgaa
    36661 tcccgaccat cggcgatgat agcccccgta atgcgtggtc cttatttccg ataagtttac
    36721 ttgagtcttt agcttcccat ttcagcccac tctccactgt gtgggctttt ttcattggtc
    36781 tgacccgtcc tgaatagcca aacaatggca atgttccact ttgcagggat gataataagg
    36841 ggatctgatt cagaagagtt ttgaaaagtt ggaccgccat cgaggcctcg aaccccgtac
    36901 catacaacaa tcctggttgt cgtctgctct tcctgatgag ctaatggcgg tttttgatat
    36961 gagccttaca gcatattttc tgtcaggctt gagtgcgatg cgtaaggtac agaaaagccc
    37021 ccatacaaac atgatggaat tatgattttt ttcatatgaa tgtcaaccca cacattcaca
    37081 aaacatctgc atgagatcat tttttataca ccacatagct gcgaaaccac tgctggtgct
    37141 tttttaagcg aagcgtcgct gatcacaaca aaatatcggc gaaaaggcgg tggaatgcca
    37201 tttagcatac ggatatacag atgaaatgat tacctgcttt gcgattcact ctctggtgga
    37261 ataacaactt gcaggtggaa ccctttctga actaaagagt gtagcggatt ttaacgtgct
    37321 tatttacagg ataatatctg tcacctaatt cctgcactct attcgatatc cattcattta
    37381 atcgtgcgtc atctatgtat gtttttacgt ccagaacaga gagcaataac atcaccagtg
    37441 attcaggaaa aacagggtag atatttttta agtgtaataa taatccatgc gcaagcgcct
    37501 gcttaatggg cgcgtctgtc tccggaatag acgacatttc gcgaagccat ttttccacat
    37561 gcatacactc tttcccttta gggaaaagtc tcagcaaagg catatggtgt tcaagaagaa
    37621 tacatttgca atctgttcgc actgaatcga taaactgaac taatttctta agcccggtaa
    37681 agcgtccagc ctgcacattt gctttaatat caagaaacgc atcatcctgg tcatataccc
    37741 aaactgtccc tttttgattt tcaagtatgc gaaaaacaaa cttaaccggg tgatcagggt
    37801 aatccatcca gtcataaaat ccagggaaat cattaaactg cacatttatg gtgagtgcgt
    37861 tatcgtctga ccactcacag tctcgtacgg cagccatgag atgaatgagt ttgtcgtcat
    37921 cgactaaatc atcacgacaa tcataaggat aatcataata ttgtttaatc aaaaccttga
    37981 atatatcgac acaggaaacc agggagcttt tgatatcctc tggtaactga ttgtttttaa
    38041 ccgtattaag tatgctttgt tgcgtatatg gcatcaaatt attgtgcgga gcttttaatg
    38101 atatcatcgc gatctctctt aaataaaaat aaacgtcgca gataataaag ctctgattaa
    38161 accaggcact atcagaaatc gctcataact aacgtgtgaa gtattgtgta ctggagggag
    38221 ttaatttaac aatttgaatt tacgttatag gttttactgc tactttgata ttatttcgct
    38281 ctccttaact aaaaagttaa gtggagagcg aagttaatat gttaaaccac agcgtattta
    38341 ttaatttttt cagtctgtta gtgtgcgatt atcgataaca aaacgatatt tcacatcacc
    38401 acgcagcatt cgctcatagg cttcattaat ttgatcggcc cgaatcatct ctatatcagc
    38461 cacgatgcca tgttcggcgc aaaaatcgag catctcctga gtttctggaa tgccgccaat
    38521 catagaaccg gctatcgcac ggcgtttcat gatcaggttg aaaacttccg gcgatttatg
    38581 cggtgtcgca ggcgcaccaa ccagcgtcat ggtgccatca cgcttcagca aggtggtaaa
    38641 atcgtcgaga ttatgtggcg cagctactgt attcaaaatg aaatcgaaac tcttcagatg
    38701 agccgccatc tcatcggcat tgcgtgagtt aacaacttca tcggccccca gggcttttgc
    38761 cgcttcgcgt tttgcctcag aagtggtaaa tgccaccaca tgcgccccca tcgcgtgggc
    38821 cagcttgatc cccatatgtc ccagaccgcc gatgccgacc acgcccactt ttttacccgg
    38881 cccggcctgc cagtgacgta gcggcgaata cgtggtgatc cctgcacaca acaaaggagc
    38941 caccgccgcc agctgctctt gcgggtgacg aatacgcaga acatatcgct catgaacgac
    39001 gatctgttgt gagtagccgc ccagagtatg gcccggttcg tccggcgtcg gcgagttata
    39061 ggtgccggtc atgtgatcac agtagttttc cagcccgtct tcacactctt cgcaatgttt
    39121 acaactgtcg acaatgcagc cgacaccgac cagatcgccc ggcgcatgtt tttctacctg
    39181 atcaccaacg gctaccacac gccccacaat ttcatgaccc ggtacgcagg ggtaaaccgt
    39241 ccccgcccac tcggaacgga cctggtggag atcggaatgg cagacgccac agtaagcgat
    39301 ttcgattttg acatcattcg gtcccggttc acgccgggtg atatccatcg gttcaagtgg
    39361 ttgtttagcg gaatatgcac caacagcttt gatcttcatt gtgtttactc ctgattagct
    39421 atgtgtattt ggcgatgcct gatatgtcat cgcggcaaaa cgcgtccatt gaatatagcc
    39481 aatatttaaa ccacccgatc cagccccaac atgccgcgag cctgaatgaa acaaagcagc
    39541 aacgttcccc ataaggcgag cacctgtaag tgtgatattt gctatccgta aaagactaaa
    39601 atcggcaaaa ctgatgatct tttcacgtag acaaatagtt atctacacaa cgtcaataag
    39661 ggcatattcc tcaaagataa tatccatttc tgccgacaca ctcatattca ggttgcccca
    39721 ctccctgggg cttaaatctc ttactattca ccgttgcagg cgtttcaaat gcgcatcaac
    39781 ctgccacact cccccacacc agttgccctt tatggaacgt tgcggttctc ggcgagatac
    39841 gcgccaccgc ctcggcggaa caggaggcgt ccaccagcac aaaactggcg tcatcctgcg
    39901 ctttgggcca cacgcgctcg cctttttcgt tgagcggcaa tacatcgcca gtggctaaaa
    39961 acagcgagcg ggagaggttc tgttcgttag gacgaatata gagctgcgcg tacagattgg
    40021 ctttttccag catgtcgccc aggccgtaag gcgaccagtg gtcgataacg ctgtcagtgc
    40081 cagtcatcac ttttacgcct ttgtcgtgca actgtttgag cggcatatgc agcgtgccaa
    40141 tcggcaccgt cgaggcgata gaaatttgct gcgccgccat ccggtgcgcc agttcatcta
    40201 cctgttgttc gttgagcgtt gccagcgcaa aggcgtgact gatggtcagc ttgcccttca
    40261 gctgtggcgt tttctctacc gtttcaacca tataattgat ggctgccacg cctgccggag
    40321 tggtttcatg caggtgaata tcgacgcctt tgtcgtagtc cagcgcaatc tgaaacatgg
    40381 tgtcgaggga tttttccatc gcgccatcaa cactggtcgg gtccagcccg ccgacgtaat
    40441 gcgcacccgc ctgcatcgct tcgcgcatta agggttccga tttcgacagc agcaaaccgt
    40501 gctgcgggaa ggcgacgatt tcacactcga agcccgcctg acgccgcgcc agcaccgcct
    40561 gcaaattttg cagatttttt aggccggaaa ccggttcgat attgcagtgg ctgcgggcaa
    40621 tggtggtgcc tttcgactgc aatagatcga tcagtttttc cgcccgctcc tgcgtatacg
    40681 gttgtaactc cggcagcatt ttctgctcga gtttgatcat gtcctggata gtggtgcctg
    40741 ccggacgatt gagcgagcgc cacggcccgc cgtagaaggt tttgtcgaga tgaatatgca
    40801 tgtcgcgagt ggtgggtagc atcaacttac cgccagcgtc atagtgcggc agcgtggcgt
    40861 ccggatgctg cttgttctcg cgcagggcga caattttgcc gtcctgaatc tccacggtct
    40921 ggcgcgccgt gcgggtctgc accgccacac cgttttcgta gtcaaaaccg gtttccagca
    40981 gcacgttatc gagatagtaa tgcgggtcag tgattttcat ggtgttgttc gcttcgcagt
    41041 ttagggtgcc agctaccgcc gcatgggcga gcggcacaga ggtaccaaac agcgcggcgg
    41101 cggtgaccat cttaccgctc tggctcagaa attcacggcg gctattgctt tctttcatcc
    41161 tacgtcctta cgttttaata atatgggtgc ccgtttcacc gcgcagcgcc gcaggcaggc
    41221 attccggcgt ggtgataatc acttctttgc cgccctgctc caggaacgtc aggctggcga
    41281 tgatttttgg caacatgctg ccgggcggaa aatgcccttc ctgcatatag cgagtcatgg
    41341 tggcaatatc cacccgatcc agcgcctgct gctgcggttt accaaagtgg atacagactt
    41401 tttcgacgcc ggtggtgatc accagaatgt cggcgtgaat ttcacgggcc agcagcgcgg
    41461 tagagagatc tttgtcaata accgcgtcca cgctttggta atcccccgct tcagtacgca
    41521 ctaccggaat tccaccgccg cccgcgccaa ttacgacaaa accttgttgg atcagcgcct
    41581 taatggcggg tgcttcgaca atacgtttcg gttccggcga ggcgaccacg cggcgatagc
    41641 cccgtccggc atcttcaaca aaacaccagt cagggtttgc cttttgtagt ttgtcacgct
    41701 ggctttcact gaagaatgca ccaatgggct tggtgggatg ggcaaaacct ggatcgtttt
    41761 tatccacttc cacctgagtt accacggtga cggctttttt ctcgccgtga cgcgccagcc
    41821 ggttattcag cgcctgttgg atcagatagc cgatgccgcc ctgcgtatct gccacacagt
    41881 tcgccagcgg cgttaagggc agcccttcgc gctcgtgggc aatctccgca cggcgtaaat
    41941 ccagcccgac ctgcggcccg ttaccgtggg tcagcacaat gtcgtaatcg gaagccagca
    42001 tttccagcac cgtatcggcc acggctttca ccgcctccgc ctgatgctca atcgactggc
    42061 tggcgttatc tttgataatg ctgttgccac caatggcaac gaccacaagc tctttcatgt
    42121 tgttatgtcc gctacagagg gtgattaggt tatgaccaac gccagactgc ctgatgcgct
    42181 acgcttatca ggcctacaat tctcgggcca tccgtaggcc ggataaggcg ttcacaccgc
    42241 atccggcata aacatgtaag tcagacgccg taacgttcgc accagccgag aatggctttc
    42301 tcgaagcagg cgaatggtgg atgcacaatg cccgcgccaa tcatgccgac gcccgcatct
    42361 ttatgggcga tggcggtgtt gatcaccggc agaatgccgc tgctacccac gcgggtgatg
    42421 tcgattgccg acggcacgcc cataaaaccg agcagcggaa tggtgacgtt ggggttttcg
    42481 ccaagggtga tttcgcgcat ctgacgggag aaatcgatag cctcttccac cgtgccgccc
    42541 accagtgcga cgattgcagg cgcggtcgcc atggcaaacc cgccaatacc gtaggtttct
    42601 gtgatggcgc tgtcgccgat atccagcccc gaatcttccg gcttataacc agcaaacatc
    42661 gggccgatga cctgctgcgc tgggccggta aaccactgcc ccggcaggcc gctgacccgc
    42721 aggccgaact cgacgccgtt acgcgccatg gtggtgacca cggtgctgta ttcgatgccg
    42781 tgcgccgcat ccatcgccgc tttacacatc gccatccacg tcgggccgga gaagtagtcg
    42841 ctgctggcga caaactcgaa cacttcgcgc tgttgctcga cggaatagcc tgcctgaatg
    42901 atccccggcg tcagtgcctg aatcagcagc gtcgtcccgg cgttattgcg gttatggcac
    42961 tcatcgccca tatgcagcgc ctgcgccagc attaagcgca gatcgatctc accgataatt
    43021 ttcatcgcgt cgcgcagcat tggcccctgc acatcgcgca tccagttgag gcggtcgatc
    43081 acgctctgat cgttggcacc catacgcaaa atcttcgcca tctgctcgct catattggta
    43141 taagcgatgt tgccgtaggt tttgtttttc acgatgtgca taaacatcga ggccgaggta
    43201 acgcccgcca tcgatcccac gcagtcgtgt tcgtggcacg gcgagaaggt gatctccccg
    43261 gaggccgcca gttcagtcgc ctcatcgaga tctttcgcca gcccttcgaa caccaacgcc
    43321 ccggtgaccg cgcctttcat cgcgccgcac attttttccc aggtgatcgg cggcccggcg
    43381 tgaagaatgg ttttcggcgt catgcccggc accacgttaa tcgcctgatc aaaaccaatc
    43441 agcaccggat gcgactggat aatgcgctcc agcgcctgct ggttagcggc ggcgattttg
    43501 tctgccagcg gcgaatcggc aatgttatcc agcgcctgca ccacctgcat attgccctgc
    43561 cccggcggcg tccagtcgag ttgagtcact tctacatgct gctttttcag gtcatcgcta
    43621 aacatggcga taccgacgtt aataacgttc aacggttggc taaacagtga ctggctcatc
    43681 aggcttcctc ccctttgcag ataaattcac gcgccagcaa tccggtattg gtgctgctgc
    43741 tcgccagaat cactccggca tcaagcagca tctggctttg ttgttctaat gatggcgtat
    43801 cgagatcggt gcccagcaca taggcgagaa tgatcaactc gcgtccttcg gcggcggcga
    43861 tcgctttcgc ttctttgatc gcctcgatgg tggagccgac cggatcttca tgcgatccaa
    43921 atccgagcac aaaatccatc acgatcaccg ccacttctgg atcgcgcgct tcttcgatca
    43981 agcgactgat gcggttggtg gggtcaatca ttggatgcgg cttgccgttg gtgaagtcgt
    44041 catcgccaaa gtcgaggaag gtgtgtttga tgctgcggtt gatatcttgc aggcggaatt
    44101 caggatcggg ctgaatgttg ctgtagacat cgccatgttt ttccatcacc gcgaacatgg
    44161 tttcgtcgca cagcgtgccg ccgcagaaca ggccacgtat gtatttctgc tgcggttgca
    44221 gacgcgcacg cacatcagca atcaacggct ggttaagcgt atgcaggtcg agattttcct
    44281 gtttcacgcc ggagagcatc accgctttta gcgccgcctc tttgctgccg cgggcaaact
    44341 gtagcccctg ctcatccacc ggcgtttcaa cgcgaccgag gaagcagacc accaccggct
    44401 tgcggcaggc gcgcgcgcgt tccagcactt tacgggccac cgcaggcgcg ggcggtttgg
    44461 agataagcac aatgatttca gtttgcggat cgttttccag catcccgatg gcgtcgagca
    44521 tcatcaggcc gccgattttc tcgctcaggt cgcgcccgcc ggtgccaatc agttgtgaaa
    44581 cgccgccacc aaattcatga atgcggacgc tcagctcctg gctgccggtg ccggatgcgc
    44641 caacaatacc gatgttaccg cgacgcacgg cgttaccaaa gcagagcgcc gcgccgttga
    44701 taatcgccgt gccgcagtcc ggccccatca tcagcagccc tttttcatgg gccaattgtt
    44761 tgagcgccag ttcatcttca accgagacgt tatcggaaaa cagcatcacg ttgagatcgt
    44821 tttgcagcgc ctgacgcgct tcgcgagcgg caaacagacc gttgaccgaa atcaccgcca
    44881 ggttactttc cgggatatgc tttttggcac tggcaatagt ggcgtaacgc gcctcgtgcg
    44941 agccgctttg cgctttggtg ttgaacagtt cttcaatctc caccagcaac tgctcgttgt
    45001 ccgcacccga tttaccattg atgacaatca tcaggtcgcc gtttttcgcc tgttccagct
    45061 ccggcgtcag cagtcctaaa tttttcagca cacctttgtt catttcggtc gccatcgcca
    45121 caaatgcctg ctcgacgccg tcgagtttat ttgcacgcgt ggagatagac atcagcgaga
    45181 cagaatcaaa ataggtattc ggtttaatga ctattttaac tgacataatt ccctccgtag
    45241 gtttggctca gcgcacaacc atcggccatg ccatacagca tgtcgcagcc ggaactggag
    45301 ccaatatgtt taattttttc tattgcctga gttgcgttcc cagggatgtc ataaataatg
    45361 ttgtgaataa aactatgaat attttcccgg caacgttgtt gtaatgcggc ttccagcgtt
    45421 atagcactta ataatgtggt attatttctg ccgcgttgca gaccgagata aaattcctct
    45481 ttgtattttt ccgccggatg cccgggaata aataaaacaa gcgccagacc tgttaaataa
    45541 tcgtcggctg atggcgtaag gccaatccct aaccccatca tactggcgac cgctgaggcg
    45601 atattttgtt tatcgtttac ggcctgaata actgcccgtc gtttaatatg taattcttta
    45661 tttaacgcct gataaaacgg attatcgccg cgataattaa acagggtttc attttccccc
    45721 aggtgctgat gaataatgtc gtgccactgt tgccaggcga taaggtgaaa attcacagcg
    45781 gtcatttgca aggttgggga cagccaacgc cgacaacgac tcatctctat atgaagatgt
    45841 tgaccaaccg taatgccttg ctcgtcaaac tgaaccttat caccatgccg gaacagacca
    45901 ttaaagtgag tgagtgccaa ccgacaactg tttggcgcat tgtcgtactc ttcacaaagc
    45961 agcgtcagca actgctggcg agccgggagt gcgatattta ccgctctggc aaagacctgt
    46021 tcaacctgtc cttgcccccg ctgcgccaga aaatccgcat ccgcagttag cgcccacaca
    46081 caccgtcttt ctggtgtgtt gatagcaagc ctcccggttt atgcacctgc ggcaatcagt
    46141 aactgcgcaa tctcttcaaa gccccgttcc cgcgccagtt ccagtggcgt tttgccatat
    46201 ttatcggtca gatgcgggct ggcaccgtgt tccagcaata actggacaat cgcctgctgt
    46261 ttaatcccgc catcattaag cacaatcgct tccagcagcg gtgtccagcc aacatggttg
    46321 gtctggttaa cgttaatttc cgtatgcgcc aaaagctctt ttacaatact taaatggcct
    46381 ttttcacagg caggcgtcag gccgacaccg ccaaaacggg tgacgcaatt aaggtctggt
    46441 ttagccggta aaataattcg tagcagcgtt aaatcaccgt tcagacagct cattaaaaaa
    46501 ggatttaaac atgtcaggtc ttgtttatta atatccgctc cggcatcaat taatgcctga
    46561 acgcaagcat attgctgata taaacttgcc agcgtaattg ccgttttccc ctggcgatcg
    46621 caggtattaa tatcgacacc cagcgcaaga caggttttta ctttatcgat atcgccctgt
    46681 tgtgcagcca ataaatagtc ggcaggtaga ttttttatcg tcattcacat tcccctggtt
    46741 tttgtggctg ttttcgatga attgagaata gcaacgataa tctgataaaa gaacccgctt
    46801 aaatttaatt cgccgtattt cgcctttttg ttgaaagatg ttcaacaatc ttttcgcagt
    46861 gtgcggtagt gcaatatttg tttctttgac ccgataccct ttcactcaat caggttttat
    46921 ttctctatag tctgcacatg taagagtagc tttcgtccat gtctaaccag aggggaacaa
    46981 gatgaacgga ctcacggcaa cgggtgtcac cgtcggtatt tgtgcagggc tttggcagtt
    47041 agtgtcttca catgtcgggc tctctcaggg atgggaatta ttaggaacca ttggcttcgt
    47101 cgccttttgc agtttttatg ccgcaggcgg cggtaaatcg gggtttatca gaagtctggc
    47161 ggttaattat tccgggatgg tgtgggcatt tttcgccgcg ctggctgccg gttggctggc
    47221 ctcggtgagt gggatttctg ggttctgggc gagcgtgatt actaccgtgc ctttctcagc
    47281 ggtagtggtc tggcaagggc gtttctggtt actgtcattt attcccggtg gctttctcgg
    47341 catgacgatg ttttttgcca gcgggatgaa ctggacggtg acgttactgg gttttctggc
    47401 gggtaactgc gtggggataa tttcagaata tggtggacaa aaattaagcg aagcgacgac
    47461 taaacgcgac gggtattaaa agttattcaa aaatgattat ctatctttaa ccgatacctc
    47521 tacagtcata gctggagaaa ttcatagtag ataatggcga gaatggttta atgaactcaa
    47581 tttttaccga agaaaacctg ttggcattta ctaccgctgc gcgttttggc agcttcagta
    47641 aagccgcaga agagttgggt ttaaccactt ccgccattag ctacaccatt aagcgtatgg
    47701 agacggggct ggatgtggtg ctgttcactc gcagtacccg cagcattgag ttaacggagt
    47761 ccgggcgtta ttttttccgc aaagctaccg acctgctgaa tgatttttat gccatcaaac
    47821 gtagcataga tactatttcg cagggcatag aagcgcgtgt gcgcatttgt attaatcagc
    47881 tgctttatac gcccaaacac accgcgcgct tattacaggt gctgaaaaag cagtttccta
    47941 cctgccagat taccgtgacg accgaagtgt ataacggcgt ctgggatgcg attattaata
    48001 atcaggccaa tatcgccatc ggcgcacccg atacactact ggacggcggc ggtattgatt
    48061 acactgaaat tggcgcgatt cgctgggctt ttgctatcgc cccggatcac ccgttagcct
    48121 ttgtgccgga acccatcgcc gaaagccaac tgcgtctcta ccctaatatt atggtggagg
    48181 acaccgcaca tacgattaat aaaaaagtgg gctggttgct gcatgggcag gagtcaattc
    48241 tggtgccaga tttcaacacc aaatgtcagt gccagatttt aggtgaagga attggttttt
    48301 tgccggatta catggtccgt gaggcgatgg cgcaatcctt gctggttacc cgacaaatcc
    48361 ataacccgcg ccaggattca cgcatgttac tcgcaaccca gcactctgcc accggtcagg
    48421 tgacgcagtg gattaaaaag caatttgcgc caaatggcat attaacgggg atctatcagg
    48481 atttactgca tcgggagaat taatcgctgc caacctcccg atgcgctgtg aatagcggtt
    48541 tacatcaacc accagctttc attacccatt cagaaacaaa tttattaccc ggtactggcg
    48601 gggagtacaa atagccctgc aaaaagtgaa cgcctttacc gatcattaaa tccgcctgct
    48661 cctgggtttc taccccttcc gctacgatac tcagaccagg cttacgcgcc agttcgacaa
    48721 tattgtccac aatatgaccg gagatttcgt cgacactcgc catttgcaca aatgacttat
    48781 cgatcttaat aaaatcgacc gggaacgcct gcaagtaacg ataggtcgca taacccgtac
    48841 caaagtcatc cagcgcaaag gtaatgttgt gctgatgaag gctgtcaaat atcgctctgg
    48901 cttctggcgt taccggaata gggttacgtt ccgttagctc gagaaccagt ttgattttat
    48961 cgtttcctaa tttcttgacc aggttcaggc attctttttc aaatcccgcg gccaaaaaac
    49021 aacccgccga gacgttgata ccaatatgga aattatccgg cagcaaatgt tttaccggca
    49081 tcagaatatc cgcagtctgt ttcatcagtt ggcgggtcat tatgacgata agaccagatg
    49141 actccgccag aggaataaac tgatccggtg ggataattcc cgtttgtgga tgttcccagc
    49201 ggacaagcac ctcacagccc gtcagtaccc cagtctgcgc gcagaaaacc ggttggatcc
    49261 acggtttgaa ttcgtggttt tccaacgcca gactgatggc ttcaggcgtg acgatatggt
    49321 gatagtgatt atcattaata tatctgtgac tattactccc cgtggcggat atgatctccg
    49381 gattgtactg aaagaaaata tcgcgccaga aagtaatatc gctctgaatc cccagtttct
    49441 caaagagctg tttcttttga tgtgaaattg tctttgcact gcgatttctg tactgtgata
    49501 tttctgttac agacattccc tgcatcagca gcttgagtag cagtcgttct ttctcggata
    49561 cgctaccagg caatgacaaa tgaaccgttg taccgaactc ttgcagaaaa acacgaaaat
    49621 cacatgaatt catgaacaca cctttatctt ttatcgctgt tcagtatgag tgattacctg
    49681 tcgattcctg agcacacata aggggtaacc ataaacatca gcgatgccat acgtataaca
    49741 tcccacatca ctatcaagaa gattatcttt acctctgatg ctcgctcaaa aacagttacg
    49801 ctaaatacct taaaaaggta atcacaagtc aaactcagga gataatcacg caagaatgca
    49861 atattaaaat tcagatactc atttttggta ataaagaagt agttggacta tttcaatatt
    49921 tataatttcc cactacatac tggcatccat tcatgagcaa aggcgcatta tatgaattta
    49981 acaatccaga tcaactgaaa atacctctcc ctcataaaca catagcgtca acattcaatg
    50041 acataatgag taaagatgtt ggttatgcat acgtatcatt actctatgcc tgtcccttaa
    50101 aaacccactc attaagactg aatccattca gcaaatgata accaatatta tagcaatgcc
    50161 aataaaaaca atatttaaaa cattcaataa aatatcacac acagataatt aaaccttaaa
    50221 taacaaatgg ttgcgttaat ttcagttgat ttacgataca catttctcat gaataatgcg
    50281 tcatttcaaa tgatcagcaa tatttacatt aaataagact aaacttattg aacattcgca
    50341 acagcccaaa tgtaggtttt gggctgcaat agttcttttc ttttttatta tctgttgtct
    50401 ggtaatcatc aatagatact aagggcttaa ccgtatagat ataacgtatc aagaggtagg
    50461 agaaacagcg ccatatgcag acatggcgcg gttttatgca ataacaatca cgcgtccggg
    50521 aacatcacgc tatggcccgg cgcttcacga tggagatgaa taaagttaag gtgccgctcg
    50581 tactggtcaa gaatatcggt gatcacctgc tctttgctgt agtccatcag gtcgttgccc
    50641 tggctgcctt ctaacaggaa ggtttccagc cggtagtagg tcgatttacc gctgcgtgcg
    50701 cggtaggtaa agcccggcac cgaatattgc tgcggccaaa tctgatagac aaagttttgc
    50761 tcttcgccca tatgcaccaa caaatccaga tgccccaact gttgtccctc ttccggcggc
    50821 aggcttttta gctccacgta cgcgccgcgc aaccgcagct cctgcgccac ttcttccatt
    50881 gccgggtaac agaccgtctc catcatctgt ttagtgtaac gcgtgcccgg ataattcatc
    50941 aggcgcgaga gacgtttttt ccagctcagg cgatcctgaa gccccagcgg tcgcggcgcg
    51001 gtatcgcggt tggcactttc acggcggtaa tcttctacct tcagagattt atacaacccc
    51061 gccatcacga agaaaatcac aaagctgaac ggcagcccca tgattaccgt ggtgttttgc
    51121 agcgcggata tcccgttggt catcagcatg ccgagcgtca gcagaccaat cgccaccgac
    51181 cagaagacgc gcagccagcc gggggcgtcg ctgttgatat ctttaagctg cgaggtgaaa
    51241 ttccccagca ccagcgcacc ggagtccgcc gaggtcacat aaaacagcag gccagtaatg
    51301 gtggcgacgg aggcgctaaa ggtaaacgcc ggatactgcg ccagcaggct gtagaagccg
    51361 cgctccggat ggaccatcgc ttcctcggca aatgccgcgc cgccgtggat aatttcatac
    51421 agcgcgctat tgccgaacac cgagagccat aacagcgtga aggtaaacgg aataatcaac
    51481 gtgcccagca cgaactggcg aatggtacgc ccacgcgaga tacgcgcaag gaacaagccg
    51541 acaaacggcg accatgccac ccaccatgcc cagaagaaga gcgtccagtt attcatccac
    51601 tcaactggac ggtcgaaggc aaaactgttg agcgtcatgc ccataaagcg attcacatag
    51661 tcgccaacat tcagcaccag cgcattaagc aggaacgaag tgtcgcccat aaacaatacg
    51721 aacaggatca atcccagcgc cagcgcgaca ttaagctccg ataacacgcg aatgccctta
    51781 tcgacaccgg aggtgacaga gatcgtggcg attatcaccg acaaggcgat cagtgccgct
    51841 tttgccgcca tcgaatcggg aatatcaaac agtacgctca agccatagtt aagctgcacc
    51901 acaccgatac cgagcgtagt ggcaataccg aagatagtgc cgatcaccgc tgcaatatcc
    51961 actgagtgac ctataggtcc gttaatccgt ttaccgaaga tcgggtacag cgccgagcgg
    52021 atggtgagcg gcaaattata acgatagcta aagtatccga gcgccatgcc catcagcgca
    52081 tacatcgacc agccggttaa gccgtagtga aacagcgtcc agaccatcgc ctgacgcgcg
    52141 gcctcaatcg tctgtcccgc gccttccggc ggctgcatat actgcgttac cggttcggct
    52201 acggaaaaga acatcaggtc gataccgatc ccggcagcaa acagcatcgc cgcccaactc
    52261 agcaggctga attccggttt ggattgttct ggcccgagct tcaccgaacc aaaacgcgaa
    52321 caagcgatac agaccacaaa gacaatatag agcgttgccg ccagcagata gtaccaaccg
    52381 aaggttttag acacccagtc cagcgtgcgg ccaatccaca gggccgagaa gtcgcgaaac
    52441 aggatcgttg tcagggaaaa caacaaaatc agtccggcgg aggtgtaaaa caccaccgga
    52501 ttgattttgt ccttttccct gctgtgtgaa aggtctgtca tccagtatcc ccactgttat
    52561 tgttaccatt taaaatcaaa ttcgtaacaa ttaagacaca ttttatattg aacgtccaat
    52621 caataaccgc tttaatagat aaacaacgct gatgaatgga gtggcgaaaa tgcccaaatt
    52681 ggggatgcag tcgatccggc gcagacaact gatcgacgcc acactggaag caataaatga
    52741 agtgggcatg cacgatgcaa cgatcgcgca gatcgcccgc cgtgcaggcg tttctacggg
    52801 gatcatcagc cactatttca gggacaaaaa tggtctgctg gaagcaacca tgcgcgacat
    52861 caccagtcag ttacgtgacg cggttttgaa tcgattacat gcacttccgc agggcagtgc
    52921 agagcagcga ttacaggcga ttgttgccgg aaacttcgat gaaactcagg tgagcagtgc
    52981 ggcgatgaaa gcctggctgg cgttctgggc cagcagtatg catcagccga tgctctatcg
    53041 tttacagcag gtcagcagcc gccgcttgct gtcgaatctg gtgagcgagt ttcgccgcga
    53101 attgccgcgc gaacaggcac aggaagcggg ctacggcctt gccgcgctga ttgacggatt
    53161 atggctgcgc gcggcactga gcggcaaacc gctggataaa cctctcgcac actcgctgac
    53221 ccgtcacttt attactcagc atttacccac cgattaaccg aggagacgtg atgtcccgaa
    53281 tggcagaaca gcagctttat atacatggtg gttatacctc cgccaccagc ggtcgcacct
    53341 tcgagaccat taacccggcc aacggtaacg tgctggcgac cgtgcaggcc gccgggcgcg
    53401 aggatgtcga tcgcgccgtg aaaagcgccc tgcaggggca aaaaatctgg gcgtcgatga
    53461 ccgccatgga gcgctcgcgt attctgcgtc gggccgttga tattctgcgt gaacgcaatg
    53521 acgaactcgc aaaactggaa accctcgaca ccggaaaagc atattcggaa acctcaaccg
    53581 tcgatatcgt taccggtgcg gacgtgctgg agtactacgc cgggctgatc ccggcgctgg
    53641 aaggcagcca gatcccgttg cgtgaaacgt cctttgtgta tacccgccgc gaaccgctgg
    53701 gcgtagtggc agggattggc gcatggaact acccgatcca gattgccctg tggaaatccg
    53761 ccccggcgct ggcggcaggc aacgcaatga ttttcaaacc gagcgaagtt accccgctta
    53821 ccgcgttaaa gctggctgaa atttacagcg aagcgggcct gccggacggc gtatttaacg
    53881 tgttgccggg cgtgggcgcg gagaccgggc aatatctgac cgagcatccg ggcattgcca
    53941 aagtgtcatt taccggcggt gtcgccagcg gcaaaaaagt gatggctaac tcggcggcct
    54001 cttccctgaa agaagtgacc atggaactgg gcggtaaatc accgctgatc gttttcgatg
    54061 atgcggatct cgatctcgcc gccgatatcg ccatgatggc aaacttcttc agctccggtc
    54121 aggtgtgtac caatggcacc cgcgtcttcg ttccggcgaa atgcaaagcc gcatttgagc
    54181 agaaaattct ggcgcgcgtt gagcgcattc gcgcgggcga cgttttcgat ccgcaaacta
    54241 acttcggccc gctggtcagc ttcccgcatc gcgataacgt gctgcgctat atcgccaaag
    54301 gcaaagagga aggcgcgcgc gtactgtgcg gcggcgatgt actgaaaggc gatggcttcg
    54361 ataacggcgc atgggttgca ccgacagtgt tcaccgattg cagcgacgat atgaccatcg
    54421 tgcgtgaaga gatcttcggg ccagtgatgt ccattctgac ctacgagtcg gaagacgaag
    54481 tcattcgccg cgctaacgat accgactacg gcctggcggc gggcatcgtg acagcggacc
    54541 tgaaccgcgc gcatcgcgtc attcatcagc tggaagcggg tatttgctgg atcaacacct
    54601 ggggcgaatc cccggcagag atgcccgttg gcggctacaa acactccggc attggtcgcg
    54661 agaacggcgt gatgacgctc cagagttaca cccaggtgaa gtccatccag gttgagatgg
    54721 ctaaattcca gtccatattc taaccaggag gtttatttgc aatttgacta catcattatt
    54781 ggtgccggct cagccggcaa cgttctcgct acccgtctga ctgaagatcc gaatacctcc
    54841 gtgctgctgc ttgaagcggg cggcccggac tatcgctttg acttccgcac ccagatgccc
    54901 gctgccctgg cattcccgct acagggtaaa cgctacaact gggcctatga aacggaacct
    54961 gaaccgttta tgaataaccg ccgcatggag tgcggacgcg gtaaaggtct gggtggatcg
    55021 tcgctgatca acggcatgtg ctacatccgt ggcaatgcgc tggatctcga taactgggcg
    55081 caagaacccg gtctggagaa ctggagctac ctcgactgcc tgccctacta ccgcaaggcc
    55141 gagactcgcg atatgggtga aaacgactat cacggcggtg atggcccggt gagcgtcact
    55201 acctccaaac ccggcgtcaa tccgctgttt gaagcgatga ttgaagcggg cgtgcaggcg
    55261 ggctacccgc gcacggacga tctcaacggt tatcagcagg aaggttttgg tccgatggat
    55321 cgcaccgtca cgccgcaggg ccgtcgcgcc agcaccgcgc gtggctatct cgatcaggcc
    55381 aaatcgcgtc ctaacctgac cattcgtact cacgctatga ccgatcacat catttttgac
    55441 ggcaaacgcg cggtgggcgt cgaatggctg gaaggcgaca gcaccatccc aacccgcgca
    55501 acggccaaca aagaagtgct gttatgtgca ggcgcgattg cctcaccgca gatcctgcaa
    55561 cgctccggcg tcggcaacgc tgaactgctg gcggagtttg atattccgct ggtgcatgaa
    55621 ttacccggcg tcggcgaaaa tcttcaggat catctggaga tgtatctgca atatgagtgc
    55681 aaagaaccgg tttccctcta ccctgccctg cagtggtgga accagccgaa aatcggtgcg
    55741 gagtggctgt ttggcggcac tggcgttggt gccagcaacc actttgaagc aggtggattt
    55801 attcgcagcc gtgaggaatt tgcgtggccg aatattcagt accatttcct gccagtagcg
    55861 attaactata acggctcgaa tgcagtgaaa gagcacggtt tccagtgcca cgtcggctca
    55921 atgcgctcgc caagccgtgg gcatgtgcgg attaaatccc gcgacccgca ccagcatccg
    55981 gcgattctgt ttaactacat gtcgcacgag caggactggc aggagttccg cgacgcaatt
    56041 cgcatcaccc gcgagatcat gcatcaaccc gcgctggatc agtatcgtgg ccgcgaaatc
    56101 agccccggtg tcgaatgcca gacggatgaa cagctcgatg agttcgtgcg taaccacgcc
    56161 gaaaccgcct tccatccgtg cggtacctgc aaaatgggtt acgacgagat gtccgtggtt
    56221 gacggcgaag gccgcgtaca cgggttagaa ggcctgcgtg tggtggatgc gtcgattatg
    56281 ccgcagatta tcaccgggaa tttgaacgcc acgacaatta tgattggcga gaaaatagcg
    56341 gatatgattc gtggacagga agcgctgccg aggagcacgg cgggatattt tgtggcaaat
    56401 gggatgccgg tgagagcgaa aaaatgagtc gtgatgtgaa ctaacgcagg aaccgatcag
    56461 attgagagtt accgttccag agagggggac cgaatcctta tataaacact gaggtaactc
    56521 tcatgcttca tatccagtat tcatgatgcg ggctttttgc tgatatttaa cccgaacatt
    56581 cctgatacag actattaata caaccttatc tgcgacagat tttattttct ggatatatta
    56641 tgcgtgaaca aatcaaacag gatatcgatc tgattgagat tttattttat ctgaagaaaa
    56701 agattcgtgt tatccttttt attatggcta tatgtatggc tatggtgctg ttgtttctgt
    56761 atatcaataa agacaatata aaagtgattt acagcctaaa aataaaccag acaacgccag
    56821 gtatacttgt tagctgtgat agcaataata attttgcctg tcagactaca atgactgaag
    56881 atgttattca gcgaattact acattttttc acaccagccc agatgtcaag aacagagaaa
    56941 taaggctgga atggtcagga gataagagag ctttaccaac tgctgaagag gaaatatctc
    57001 gcgtgcaggc ctctattatc aaatggtatg cgtcagaata tcataatggc aggcaagttc
    57061 tcgatgagat acaaacgcct tcagcaatta acagtgagct ttatacaaaa atgatatacc
    57121 tgaccaggaa ctggtcattg tatccgaacg gtgatggctg tgtaactata agctcaccag
    57181 aaataaaaaa taaataccct gctgccattt gcctggctct gggatttttt ctaagcattg
    57241 taatttctgt aatgttttgc cttgtcaaaa aaatggtaga tgaataccaa caaaactctg
    57301 ggcagtaata aacaacattc tgatagagag tgcctgtata aagctgaagt gacaatgacc
    57361 gggaagggaa cccgttttca ggaatggatg cctgtctttt tcactgtaaa tatcctggca
    57421 ttggtaatac caggatattt acagaagagc agcataacca tgacggaacg gggttaagtc
    57481 aggttgtcct aaacgctatt ttcaacctcg tatgccttct tcaggtttat gtccagactt
    57541 catatctctc tcaacaatcc tcaataatca gatacaccgc tttcaccggg ccatgaactc
    57601 cgacgacttt gataagctca atatccgccg ttgaactggg gccgctaatg atgttaatgc
    57661 aggaaggcat tcgttcaccg gcctgcgctt tctgatgcaa tttttctgcg agttgcgcta
    57721 cacgcggcag gatagtgctt ttacgcagga taaaaagaga atattccggg agcaggctca
    57781 atgaacgccc gcgctcggcg gcggaaaaaa gaatcacgcc tcccgattcg gttaaaccat
    57841 attcagcata cacaacaccc actttagcct gctctgcctg cgagatattc tcggcacctt
    57901 tcgccggatc ccaaacaacg gcattgcatt cctgctgcaa acgttcgcta atccccaatt
    57961 cctccagcct cgtgtcaccg ctaatcacga ccgactgatc tcccagctct ttacacagac
    58021 gtattgcagc ttctgccgcc ttcgcctcgc tggtcagctc acagcgcgtc aacataacat
    58081 cgctggcaaa ctgaataaac gcgtcacagc gctgctgttg gttaagttgg gtaagccgct
    58141 cgttagcata gttgttaagc ggcgcatctt ctgcttgcgg ttcaagtcgc agcgggcgac
    58201 ccagtgcctg agcaacgtta ttcaaaaatt cgcctcgatt atccattctt tttctcctgc
    58261 gcctgatgtt tcttaaacca actacggaaa ctctctccgt cagcttcagg aagatcgcgt
    58321 gcttccatcc agtcgctaat cgcgccaaat ttgagtggtg ttttgccgcc attgataaac
    58381 cagcttgccg catgagcacc ggccatcatc ccgactttcc acaatcctgg atgactattg
    58441 gcataagcga acatttttat cgcccgttgc tctgcttttg cggtgatccc tttttcagcc
    58501 atcacccgac gatgacgcaa aatcagtttt gacagcggaa tacgcaccgg acacacgttg
    58561 tcacaagctg tgcataaaga gcaggcgtag ggtaaatctt taaaatcttt atagccgcca
    58621 agtagcggag aaatcaccgc accaattggc cctggataaa tagagccata tccatgaccg
    58681 ccaatatggc gatatgccgg acaagtattc atacaagccc cgcagcgaat acagcgcagc
    58741 acatcccgaa attcagaggc cagcacctca gaacgcccgt tatcgacaat aaccagatga
    58801 aactcttcag gaccatcaac gtgcccagct tcgcgcggtc ctgtcagcca ggtgttgtat
    58861 cccgtcaaac gtgcaccaac ggcactgcgc gccagcatgg tgatcaatac atctacctcg
    58921 gcaaacgtgg gggcaatacg ctccattccc atcactgcaa tatgcgtttt aggcagcgtg
    58981 gtacacattc gcgcattacc ttcattggtc accaggcata ccgaaccggt ctctgccacc
    59041 gcgaaattac agccggtaat acctatttca gcactgagga aatcttcgcg gattttttgc
    59101 cggatgaata aggtcatcgc ttcaggcgtt tccggcccct catagcccag acgttcgtgt
    59161 agcactcgac ggatctgatg gcgatcttta tgaattgccg ggaccacaac atgagatggc
    59221 ggatcttgat ccagctggag aatatattca cccagatcgg tttcaatcac ctgaatgcca
    59281 gcatcctgca acacatgatt gacaccaatc tcttcggtca ccatcgattt agatttcacc
    59341 accttccggg catttttgcg ttgggcaacc tgtaaaatgt agcgggtagc gtcttctttg
    59401 gttcttgcaa aatagacgtg accgccgttt tgcgtcactt tttctgagag ctggtacaga
    59461 taagcgtcga gattactcag aacatgatca cgtatctggg cggcccgatc gcgccactcc
    59521 tcccagtgcc ccaattcatc gaccattttt tgccgatttg ccccaatacg ctgctgcgcg
    59581 tttgccaccg ctttgcgcat gatcggatct tcaatttgct gacggatgcg tgtcttaaaa
    59641 tctgtattac tggttttgat cgacatcttt atatcctcag cggctcatca acacttcagc
    59701 aatatgcatc actttgactt tctgcccttc ccgttgtaat cgcccactga tgtttagcag
    59761 gcaactcacg tcagcaccaa ttaaatactc agggcggact tccatcaggt gcgcaacctt
    59821 ttctttcacc atctcgccgg atatttcggc cattttgacc gagaacgtgc cgccaaatcc
    59881 gcagcaggta tcctgttcag caaaggtaaa cagctccagt ccacgcacat ttttcagcag
    59941 cgtaagtggc tcgtccttca ctcccagctt acgggccagg ctacaagatg ggtgatacac
    60001 cgctctccct tgcaaactgg cacctacatc gactacccct aatttattaa caataaaaga
    60061 ggtgagatcc tgcatacgcg cggcaacctt tgcggcacgt gatgcccatt caggttcatc
    60121 cgccagatac atcgggtaac tttttacggc ataggtgcag gagccagccg gtgaaataat
    60181 gggatcatcg ttatcctcca gcgcggcgat cagatttttc atccctggaa tcgcttcttt
    60241 gatataaccg ctattgatcg caggctgacc gcagcatccc tgtttctccg ggaaatttac
    60301 gcgacagccg agtttttcca gtagcagcac ggagtctcgt gccattcttg atttcagggc
    60361 gtcaccaata caggtgacaa agaaattgac attcaaaact aatactccat tacttcatgc
    60421 ccatttatgc ccgatgtttc ccccccaccg ggggatcaat catcaataaa tatggcgttc
    60481 ttttttaatt ggctagcaac ataccacccc tcatttgtat gacaaatgat aatctttcat
    60541 ccaaacgtca aatgcaataa ctaattttat ttctttcggc atcgctcaca ttttattggg
    60601 aaatcgacga tcatttttgg cataagaaat ccattttaaa atggctacat cccttatacc
    60661 tattcagagc tacttattca ggggttattg agatatacac tgatggtgta caaaaaaatc
    60721 accgacaata atcacgtatc aagtaatagt cgggatttta tttaattgaa gcaatttagt
    60781 ttgcattttt atttaaaaac tgagctagag tccatcagaa aaaaatatat aaagcccaaa
    60841 ccaccgttgg aaatagaaat agttgaaagg ctcacaactc ccggaatgcg gattatcttt
    60901 gccctgattt ctggcggttt tattcagggt gcaagctgtc tgaccctttc ccgatattct
    60961 cccggagtac aaccaaactc gcggacaaac gccttgtgaa aagatgattc actggcatag
    61021 cctactgact cagcgatcac cacaacaggg agcatttccc gggaaaacat ctgggccgct
    61081 atttgtagac gcaactttgt taatacagcc agcggcgtgg ttccggaaac atcacggaaa
    61141 agctgggcaa aacttgcccg ggacatgtgg gcgatgctgg ccagcgattc gacggtccag
    61201 gcgtgtcctg gcatttccaa catttgctgt attaccgcac caagacgtgg atgcagaagc
    61261 aaactgagaa tgtttttctc agtattaacc tgtgcaatcc actcacgcac cgcgagggta
    61321 aagaatgtag cgcagatctg gctacacagt gcatccactc ccggcattgc caatctggat
    61381 tcctgttgta aaaacgggat cgcctccctc agccagttat attctacgct gtggttaacc
    61441 ggtgctaaaa acagcgtttc cggcaaagac gttaaaaaat aacgcgccga atgttgcagc
    61501 cgaagagtgc cgcagacaat acaggttgat tcattatcga catgactcag gcgatgggct
    61561 gaattttgtg gtagcaggac cacatttccc gggcgtaatg taaaaatctc ccccgtcggc
    61621 atttccagct tcgccgctcc ttgcgttaac gcatgccaac gaataaccga taattccccg
    61681 gcaccatgtg gaagctgcca gtcacttcct aacacgcaat tcttatcgat cgttccttgt
    61741 ggagcgttaa gcatcaacaa acggctaagg gcatccatat cagactcctg agtggttttt
    61801 g
//
LOCUS       DAJOWB010000015        60958 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJOWB010000015 DAJOWB010000000
VERSION     DAJOWB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.015"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            59..1759
                     /locus_tag="OHO85_001065"
     CDS             59..1759
                     /locus_tag="OHO85_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCQ2670479.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            1824..2519
                     /gene="queC"
                     /locus_tag="OHO85_001066"
     CDS             1824..2519
                     /gene="queC"
                     /locus_tag="OHO85_001066"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCQ2670480.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(2571..2969)
                     /gene="fadM"
                     /locus_tag="OHO85_001067"
     CDS             complement(2571..2969)
                     /gene="fadM"
                     /locus_tag="OHO85_001067"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCQ2670481.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(3063..3434)
                     /locus_tag="OHO85_001068"
     CDS             complement(3063..3434)
                     /locus_tag="OHO85_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCQ2670482.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAAPAAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(3585..5456)
                     /gene="ppiD"
                     /locus_tag="OHO85_001069"
     CDS             complement(3585..5456)
                     /gene="ppiD"
                     /locus_tag="OHO85_001069"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCQ2670483.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQTAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(5648..5920)
                     /gene="hupB"
                     /locus_tag="OHO85_001070"
     CDS             complement(5648..5920)
                     /gene="hupB"
                     /locus_tag="OHO85_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCQ2670484.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(6129..8483)
                     /gene="lon"
                     /locus_tag="OHO85_001071"
     CDS             complement(6129..8483)
                     /gene="lon"
                     /locus_tag="OHO85_001071"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCQ2670485.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(8671..9945)
                     /gene="clpX"
                     /locus_tag="OHO85_001072"
     CDS             complement(8671..9945)
                     /gene="clpX"
                     /locus_tag="OHO85_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCQ2670486.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(10071..10694)
                     /gene="clpP"
                     /locus_tag="OHO85_001073"
     CDS             complement(10071..10694)
                     /gene="clpP"
                     /locus_tag="OHO85_001073"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCQ2670487.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(10940..12238)
                     /gene="tig"
                     /locus_tag="OHO85_001074"
     CDS             complement(10940..12238)
                     /gene="tig"
                     /locus_tag="OHO85_001074"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCQ2670488.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(12582..12899)
                     /gene="bolA"
                     /locus_tag="OHO85_001075"
     CDS             complement(12582..12899)
                     /gene="bolA"
                     /locus_tag="OHO85_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCQ2670489.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            13204..13782
                     /locus_tag="OHO85_001076"
     CDS             13204..13782
                     /locus_tag="OHO85_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCQ2670490.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            13826..15301
                     /gene="ampG"
                     /locus_tag="OHO85_001077"
     CDS             13826..15301
                     /gene="ampG"
                     /locus_tag="OHO85_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCQ2670491.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            15761..16708
                     /gene="cyoA"
                     /locus_tag="OHO85_001078"
     CDS             15761..16708
                     /gene="cyoA"
                     /locus_tag="OHO85_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCQ2670492.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            16730..18721
                     /gene="cyoB"
                     /locus_tag="OHO85_001079"
     CDS             16730..18721
                     /gene="cyoB"
                     /locus_tag="OHO85_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCQ2670493.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            18711..19325
                     /locus_tag="OHO85_001080"
     CDS             18711..19325
                     /locus_tag="OHO85_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCQ2670494.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            19325..19654
                     /locus_tag="OHO85_001081"
     CDS             19325..19654
                     /locus_tag="OHO85_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCQ2670495.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            19666..20553
                     /gene="cyoE"
                     /locus_tag="OHO85_001082"
     CDS             19666..20553
                     /gene="cyoE"
                     /locus_tag="OHO85_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCQ2670496.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAV"
     gene            20705..22069
                     /locus_tag="OHO85_001083"
     CDS             20705..22069
                     /locus_tag="OHO85_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2670497.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(22197..22688)
                     /gene="yajQ"
                     /locus_tag="OHO85_001084"
     CDS             complement(22197..22688)
                     /gene="yajQ"
                     /locus_tag="OHO85_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCQ2670498.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            22856..23767
                     /gene="panE"
                     /locus_tag="OHO85_001085"
     CDS             22856..23767
                     /gene="panE"
                     /locus_tag="OHO85_001085"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCQ2670499.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVATPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            23730..24320
                     /gene="yajL"
                     /locus_tag="OHO85_001086"
     CDS             23730..24320
                     /gene="yajL"
                     /locus_tag="OHO85_001086"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCQ2670500.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(24374..25822)
                     /gene="thiI"
                     /locus_tag="OHO85_001087"
     CDS             complement(24374..25822)
                     /gene="thiI"
                     /locus_tag="OHO85_001087"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCQ2670501.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            26028..26270
                     /gene="xseB"
                     /locus_tag="OHO85_001088"
     CDS             26028..26270
                     /gene="xseB"
                     /locus_tag="OHO85_001088"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCQ2670502.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            26270..27169
                     /gene="ispA"
                     /locus_tag="OHO85_001089"
     CDS             26270..27169
                     /gene="ispA"
                     /locus_tag="OHO85_001089"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCQ2670503.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            27194..29056
                     /gene="dxs"
                     /locus_tag="OHO85_001090"
     CDS             27194..29056
                     /gene="dxs"
                     /locus_tag="OHO85_001090"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCQ2670504.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            29236..30210
                     /gene="yajO"
                     /locus_tag="OHO85_001091"
     CDS             29236..30210
                     /gene="yajO"
                     /locus_tag="OHO85_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCQ2670505.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSFSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGRNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(30264..30782)
                     /gene="pgpA"
                     /locus_tag="OHO85_001092"
     CDS             complement(30264..30782)
                     /gene="pgpA"
                     /locus_tag="OHO85_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCQ2670506.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(30760..31737)
                     /gene="thiL"
                     /locus_tag="OHO85_001093"
     CDS             complement(30760..31737)
                     /gene="thiL"
                     /locus_tag="OHO85_001093"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCQ2670507.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(31815..32234)
                     /gene="nusB"
                     /locus_tag="OHO85_001094"
     CDS             complement(31815..32234)
                     /gene="nusB"
                     /locus_tag="OHO85_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCQ2670508.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(32254..32724)
                     /gene="ribE"
                     /locus_tag="OHO85_001095"
     CDS             complement(32254..32724)
                     /gene="ribE"
                     /locus_tag="OHO85_001095"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCQ2670509.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(32813..33916)
                     /gene="ribD"
                     /locus_tag="OHO85_001096"
     CDS             complement(32813..33916)
                     /gene="ribD"
                     /locus_tag="OHO85_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCQ2670510.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(33920..34369)
                     /gene="nrdR"
                     /locus_tag="OHO85_001097"
     CDS             complement(33920..34369)
                     /gene="nrdR"
                     /locus_tag="OHO85_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCQ2670511.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            34520..35059
                     /locus_tag="OHO85_001098"
     CDS             34520..35059
                     /locus_tag="OHO85_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCQ2670512.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            35358..36242
                     /gene="tsx"
                     /locus_tag="OHO85_001099"
     CDS             35358..36242
                     /gene="tsx"
                     /locus_tag="OHO85_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCQ2670513.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINDIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(36419..36766)
                     /locus_tag="OHO85_001100"
     CDS             complement(36419..36766)
                     /locus_tag="OHO85_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCQ2670514.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(36895..37866)
                     /gene="secF"
                     /locus_tag="OHO85_001101"
     CDS             complement(36895..37866)
                     /gene="secF"
                     /locus_tag="OHO85_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCQ2670515.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(37877..39724)
                     /gene="secD"
                     /locus_tag="OHO85_001102"
     CDS             complement(37877..39724)
                     /gene="secD"
                     /locus_tag="OHO85_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCQ2670516.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(39752..40084)
                     /gene="yajC"
                     /locus_tag="OHO85_001103"
     CDS             complement(39752..40084)
                     /gene="yajC"
                     /locus_tag="OHO85_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCQ2670517.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(40107..41234)
                     /gene="tgt"
                     /locus_tag="OHO85_001104"
     CDS             complement(40107..41234)
                     /gene="tgt"
                     /locus_tag="OHO85_001104"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCQ2670518.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(41290..42360)
                     /gene="queA"
                     /locus_tag="OHO85_001105"
     CDS             complement(41290..42360)
                     /gene="queA"
                     /locus_tag="OHO85_001105"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCQ2670519.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            42453..43034
                     /gene="acpH"
                     /locus_tag="OHO85_001106"
     CDS             42453..43034
                     /gene="acpH"
                     /locus_tag="OHO85_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCQ2670520.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(43039..44856)
                     /gene="malZ"
                     /locus_tag="OHO85_001107"
     CDS             complement(43039..44856)
                     /gene="malZ"
                     /locus_tag="OHO85_001107"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCQ2670521.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(45012..46385)
                     /gene="proY"
                     /locus_tag="OHO85_001108"
     CDS             complement(45012..46385)
                     /gene="proY"
                     /locus_tag="OHO85_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCQ2670522.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(46461..47780)
                     /gene="brnQ"
                     /locus_tag="OHO85_001109"
     CDS             complement(46461..47780)
                     /gene="brnQ"
                     /locus_tag="OHO85_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCQ2670523.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(48187..49482)
                     /gene="phoR"
                     /locus_tag="OHO85_001110"
     CDS             complement(48187..49482)
                     /gene="phoR"
                     /locus_tag="OHO85_001110"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCQ2670524.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLGGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(49540..50229)
                     /gene="phoB"
                     /locus_tag="OHO85_001111"
     CDS             complement(49540..50229)
                     /gene="phoB"
                     /locus_tag="OHO85_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCQ2670525.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            50419..51621
                     /gene="sbcD"
                     /locus_tag="OHO85_001112"
     CDS             50419..51621
                     /gene="sbcD"
                     /locus_tag="OHO85_001112"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCQ2670526.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            51618..54761
                     /gene="sbcC"
                     /locus_tag="OHO85_001113"
     CDS             51618..54761
                     /gene="sbcC"
                     /locus_tag="OHO85_001113"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCQ2670527.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRQDELQQEASRRQQALQQVLAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDALPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            54887..56071
                     /gene="araJ"
                     /locus_tag="OHO85_001114"
     CDS             54887..56071
                     /gene="araJ"
                     /locus_tag="OHO85_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCQ2670528.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYMMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(56214..57122)
                     /gene="mak"
                     /locus_tag="OHO85_001115"
     CDS             complement(56214..57122)
                     /gene="mak"
                     /locus_tag="OHO85_001115"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCQ2670529.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            57247..58158
                     /gene="rdgC"
                     /locus_tag="OHO85_001116"
     CDS             57247..58158
                     /gene="rdgC"
                     /locus_tag="OHO85_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCQ2670530.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(58236..58319)
                     /locus_tag="OHO85_001117"
     CDS             complement(58236..58319)
                     /locus_tag="OHO85_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670531.1"
                     /translation="MTQRPWSKLQRKKHNIAALKIIARRSE"
     gene            complement(58316..58651)
                     /locus_tag="OHO85_001118"
                     /pseudo
     CDS             complement(58316..58651)
                     /locus_tag="OHO85_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(58806..59090)
                     /gene="ppnP"
                     /locus_tag="OHO85_001119"
     CDS             complement(58806..59090)
                     /gene="ppnP"
                     /locus_tag="OHO85_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCQ2670532.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(59162..59839)
                     /locus_tag="OHO85_001120"
     CDS             complement(59162..59839)
                     /locus_tag="OHO85_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCQ2670533.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNRGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(60097..60288)
                     /gene="yaiA"
                     /locus_tag="OHO85_001121"
     CDS             complement(60097..60288)
                     /gene="yaiA"
                     /locus_tag="OHO85_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCQ2670534.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(60338..60862)
                     /gene="aroL"
                     /locus_tag="OHO85_001122"
     CDS             complement(60338..60862)
                     /gene="aroL"
                     /locus_tag="OHO85_001122"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCQ2670535.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
BASE COUNT        14581 a        16273 c        15275 g        14829 t
ORIGIN      
        1 tcacctgata tatacggaag taatagtgaa tagttaaata atattgttcc gggtttatat
       61 gcgattgctc aaccgtctta accagtatca acgtctgtgg caaccttcag ccggaaagcc
      121 gcaaaccgtc accgtcagcg aactggccga acgctgtttt tgcagcgaac gccatgttcg
      181 tacgctgttg cgtcaggcac aggaggcggg atggctggag tggcaggcgc agtcaggacg
      241 cggaaagcgc ggacaattac gctttctggt cacgccggaa tcgctacgca atgcgatgat
      301 ggaacaggca ctggaaaccg gaaagcagca agatgtgctg gagctggcgc aactggcccc
      361 aggtgagctg cgcactctgt tacagccgtt tatgggcgga caatggcaaa acgatacacc
      421 cacgttgcgt attccctact atcgcccgct cgaaccgcta caaccaggct ttttgcccgg
      481 ccgtgccgag cagcatctcg ccgggcagat attttccggc ctgacccgct tcgataataa
      541 tactcagcgc ccgattggcg atttagcgca tcactgggaa acctctactg acgggttacg
      601 ctgggacttt tatcttcgtt caaccctaca ctggcataac ggcgatgcag taaaagcctc
      661 acacttacac cagcgattat tgatgctgtt acaactgcca gcactggatc aattatttat
      721 tagcgtgaag cgtattgaag tcacccatcc gcagtgtctg accttctttt tacatcgccc
      781 cgattactgg cttgcgcacc ggctggcgag ctattgcagc catctggcgc atccgcaatt
      841 cccactgatc ggcactggtc cttttcgctt aacacaattc acagcggagc tggtgcgcct
      901 ggagagccat gattattacc atttgcgtca tccgctgctt aaagcggttg agtactggat
      961 aactccgccg cttttcgaaa aagatttggg aaccagttgt cggcatcccg tgcaaatcac
     1021 catcggcaaa ccggaggagc tgcaacgggt cagccaggtc agtagtggca tcagtttagg
     1081 tttttgctat ttaacgttgc gcaaaagtcc ccgactctcc ctctggcagg cgcgaaaagt
     1141 gatctccatt attcatcaat ccggtttatt acaaacgtta gaagtcggag aaaacctgat
     1201 caccgccagt catgcattac tgccaggctg gactattccg cactggcaag taccggatga
     1261 agtcaaacta ccgaaaacct tgacgctggt ttatcactta ccgatagaac ttcataccat
     1321 ggcagaacgc ctacaggcga cactggcagc ggaaggctgt gaactcacaa ttatttttca
     1381 taacgcaaaa aactgggacg acacgaccct actggcacac gcagacctca tgatgggcga
     1441 cagattaatt ggcgaagcac cggaatatac tctggagcaa tggctacgtt gcgatccact
     1501 atggccacat gttttcgacg ctccagcata tgcacatctg caatcgacgc tggacgcggt
     1561 gcaagtaatg cctgatgagg aaaaccgatt taatgccctg aaagcggttt ttagccagtt
     1621 aatggcagac gcgacgctga cgccgctgtt caactatcac tatcgcatta gtgcccctcc
     1681 cggcgtgaac ggtgtgcgac tgacaccgcg cggctggttt gaatttaccg aagcctggct
     1741 tcccgcgccg tcgcaatgaa tagctggtcc gggttaatcg caggcgttac cataacgctt
     1801 ttattgttca agcaggatta tctatgaaac gtgctgtcgt tgtgttcagt ggaggccagg
     1861 attccaccac ctgtctggtg caggcattac aacaatatga tgaagtccat tgcgtgacgt
     1921 tcgattacgg tcagcgacat cgcgcagaaa tcgacgtggc acgcgaactg gcgctgaaac
     1981 tgggagcacg cgcgcataag gttcttgatg taacgctgct caacgagctg gcggtcagca
     2041 gcctgacacg tgacagcatt ccggtgcctg attatgaacc tgaagccgat ggcatcccga
     2101 atacgtttgt cccagggcgt aatattttgt tcctgacgct ggcggcaata tatgcgtatc
     2161 aggtaaaagc agaagccgta attaccggcg tctgtgaaac ggatttctcc ggctacccgg
     2221 attgccgcga tgagtttgtg aaagcactaa accatgccgt cagtttgggc atggcgaaag
     2281 atattcgttt tgaaacgccg ctgatgtgga ttgataaagc ggaaacctgg gcgctggcag
     2341 attattacgg caaactggat ttagtccgta acgaaacgtt gacctgctat aacggcatta
     2401 aaggcgacgg ttgcggtcat tgtgcggcat gtaatttacg cgccaacggt ttgaatcatt
     2461 atctggccga taaaccgacg gtgatggcag cgatgaagca gaaaaccggg ttgaggtaat
     2521 tattccgggt gtcgccggat gcggcttgag catccggcac cacaaaacgt ttacttaacc
     2581 atctgctcca gcttttcgcg caattcccct tccagagcta atgctttctg cgttttaaga
     2641 tcaatacaaa caaacgtaat aagcgcatcc gctaccacct gcccttccgg ctccagtgta
     2701 atgacctggc ttaagatgcc gcttttaccg tttaattgct gcaactgact ggtaatagtt
     2761 aacaggtcac ttaataccgc tgggcgacga tagttaatat tgatattgac cacgacgaag
     2821 gcgatgttat gggccgtcat ccactgaaaa ctgtcgctat tttccaaccc atcccagcgg
     2881 gcttcttcga gaaattcaag gtagcgggcg ttgttgacgt gctggtaaac gtcgagatga
     2941 tatccacgaa ctttgatttg tgtttgcata gcgcaataac cttacgttgt tgttataagt
     3001 acagaggtca taactggtat gacctcttca gtctggcaaa aaattgccac tatgcaaatt
     3061 aattacaggg ttaataccgc cagattacgt tccaccagcg aattgcccat ccccggcacc
     3121 tgctttagat cctccaccgt tttaaacgga ccgtactctt cgcgataact gacaatcgcc
     3181 tgcgctttct tcaggccaac gccattcatc gcgcgggcta gctcttccgc gctggcatta
     3241 ttaatgctga cccgggtgcc ttcttcgtca ctggctttcg ccggtactgc tgctttactt
     3301 tgtgctgcag gagcttccgc tttggtttct accgttgtcg gtttcgccgc aggagctgcc
     3361 gccagcgcgc tatgagacat tccggcacaa gccagagaca gggtaatgag cagtgcttta
     3421 attccgtgtt tcatactgtt ttctccttgt ttgttaacag tggaatcacc ttaacggcag
     3481 tgaaaacagt aaacaaatgg caatgttcag aaatggaaaa ggccgcgaaa gcgacctttt
     3541 cttgttacag ctgcaacgca tttttttgcg aggcggcttc aggattattg ctgttccagc
     3601 gcatcgccaa ttttgatttt cgcctcttta cgcaggttac tcatcagagc ttcaaagacg
     3661 atttgtgcgt tgttctgggt gataccctgc accatcgctt ttttctgatc ttccggcatt
     3721 gaaccttgtt tcacttcatc cagcgccagc agaaccacat taccttgcat atcggtcgcc
     3781 ataccgtagc tcggtttgtc tttcgctggc agtggcagtg caaacgccgc ctggctaatc
     3841 gggtcacgac cggaacggct taaggttttc ggctcgccaa atttcagacc ggcagcctgc
     3901 atagcttccg cacctttgcc ggctttcaaa tcaaccagca gtttctcagc atccactttc
     3961 gcctgttgtt cagctttgtt gtgctgaacc aatgccttaa cttgttcctg aacatctgcc
     4021 aacggtttca ccgcttccgg tttgtgctcg ctgatgcgca gcacgaatgc gcggtcgccg
     4081 tctacggtga tgatgtcaga gttgatgccc ggcgcgccgt tttcacctac cagaccgccg
     4141 ttaaagatag cgtcggcaac cggcttgaag ttcaactctt ccggcaggtt atctttgctg
     4201 aaccaacccg tctgagtggc tttaacgccg gcagtttgct ctgcaccggc cagagactcg
     4261 gtgtcgttgc ttgccgcatc gctcactttc tgctgcagcg catagtacgc atcgagggct
     4321 ttttcgtgtt tcactttcgc cgcaatgtcg tcacgtactt cgtctaacga tttcactttc
     4381 gctggctgaa tgtcgtccag acgtacaatc aggaaaccga ccgaagattt gatgacacca
     4441 gacagttgcc ctttttcttt cagaccagca tttttcagtt cgtcggggat agtggcatct
     4501 tctaaccaac ccatatcgcc gccgttacgg gcagagataa tatcggcaga tttttctttg
     4561 gctaatgcag caaaatcacc gcctttattc agctcatcaa gtaccgcttt cgcttcatct
     4621 tcagttttgg tctggatgat gctgtagcgg gtacgctgcg gctgggtgaa ttgatcctga
     4681 tgctgatcgt agtagctctg gatatccgca tcgctaaccg gttgctgcat cgttgcggca
     4741 tccagcttga tgtaactcac gcggaattgt tccggcgtca tgaaattgtt tttgttttgt
     4801 tcgtagtagc tggcaatctc ctgttcggtc acaggctgct tcgccgccag cgcgttaaca
     4861 tcgatagtcg cctcacgcac cacgcgttgt tgcgcgacca gtgccgccag ctcgtcggtt
     4921 tcacctttca gcataaaatc ggtaccggca acaccgttaa tcagctgttg ggtagtgagc
     4981 tggttacgca gcgcctgggc gtactgatcg gcggtcatcc ccatctggtt gaggataccg
     5041 ttatagcggc tgttatcaaa tttgccgtca acctggaagg ctggggtcgc gaaaatcgcc
     5101 tgtttaacct gctcatcact gatacccagt ttcagctcac gagcgtactg atccaacagc
     5161 gcctcgtcga tcagacgatt cagcacctgt tgacgcaggg ttttcatata gccttcgttc
     5221 gctgccagct cggagtattg atcgcccagc tgttgctgca tgcgattacg ctcgctgtta
     5281 aaggcgttct cgaactgccc acggctgatt tcctggtcat tcacttttgc ggcgtagtta
     5341 ttgcctccgc caatcaggta accactcacg ccggtcaata tgaacgacac gataatgata
     5401 ccgaaaataa tcttgagcac gagactgttt gcagccgtgc gtaagctgtc catcatggtg
     5461 taacaacact ccgctgtaga tgactgtgta cctcacgcaa cgtatcgatg cgcgcaaagg
     5521 cctattgtga caagaaacgg gggcaattgt cagcccacaa gacgcagaaa gtcaccgaat
     5581 acaaataaaa aaggcacatc agtagatgcg cccttgaact tcgtcacatc cccaccgggg
     5641 acaacgctta gtttaccgcg tctttcagtg ctttacctgc acggaagctc ggtactttag
     5701 cagcagcgat ggtgatctct ttaccggtct gcgggttgcg gccagtacgg gcagcacgct
     5761 ctttaacggc aaaagtacca aaacctacca gtgctacatc atccccttct ttcagagatt
     5821 cagttacgga agcaataata gcatctaacg cacggccagc cgcagcttta gagatatcag
     5881 cccctgcagc aatcttgtcg atcaattgag atttattcac tcttctcttc ctctttataa
     5941 tttatatcgc acttgaatcc ttcaaggtac gaacgcgcag cagttatatc aggccagcca
     6001 tccccttaca acacccctta ataagggcaa gcccgatccg ccatctaact tagcgagaca
     6061 aaaaaaggct ggcaagcccg aattagcctg ccagccctgt ttttattagt gcattttgcg
     6121 cgaggtcact attttgcagt cacaacctgc atgccagacg gttcattttg cagcgccaga
     6181 gtcagaactt cctcaatgcg cttcacagga tgaatgtcca gatcggcaat tacgttgtca
     6241 ggaatctctt ccagatcgcg tttattttcg aacggaatta gcactgtttt aatcccgccg
     6301 cgatgcgctg ccaggagttt ttctttcaaa ccaccgatcg gcagtacctg accacgcaga
     6361 gtgatctcac cggtcattgc cacatcggca cgaaccgggt taccggtcag gcaagaaacc
     6421 agcgcggtgc acatagcaat accggcactc ggaccatctt tcggcgtcgc accttccggt
     6481 acgtggacgt ggatgtcacg tttttcgtaa aaatcagggt tgatccccag tttttccgca
     6541 cgcgcacgaa ccaccgttaa cgccgcctga atagactcct gcatcacttc gccgagcgaa
     6601 ccggtatagg tcagtttgcc tttacccgga acacatgcgg tttcaatggt cagcaagtca
     6661 ccgcccactt ccgtccacgc cagaccggtt acctgaccga cacggttttc gttatcagcg
     6721 cgaccatagt cgaaacgctg aacaccgagg tagtcatgca ggttatcgcc gttaatttcg
     6781 atatgtttta atgacttatc gagcagtaac tgcttaaccg ctttgcggca cagtttggag
     6841 atttcacgct ccagaccacg cacgcccgcc tcacgggtgt agtaacgaat aatgccgata
     6901 atggcgctat cgtcgacggt cagctcacct tttttcagtg cattacgttc aatctgcttc
     6961 ggcagcaggt gacgtttggc gatgttcagt ttttcatctt cggtataacc ggagaggcga
     7021 atcacttcca tacgatccag cagcggtgcc ggaatgttca tggagttcga cgtcgcgaca
     7081 aacatcacgt cgctgagatc gtaatccact tccaggtagt ggtcgctgaa cgctacgttc
     7141 tgctctggat ccagcacttc aagcagtgcg gaggccggat cgccacgcat gtcagaagac
     7201 attttgtcga tctcatcgag caggaacagc gggtttttca cgcccacttt cgccattttc
     7261 tggatcagtt tacccggcat agaaccgatg taagtacggc ggtgaccacg gatttccgct
     7321 tcatcacgca cgccgcccag cgccatacgg acatatttac gcccggtggc tttggcgatg
     7381 gactgaccaa gagaggtttt acctaccccc ggcggcccta ccaggcagag gatcggtccc
     7441 ttgattttgt tgacacggct ttgaaccgca aggtactcaa ggatgcgatc tttcacgcgc
     7501 tccagaccat aatggtcggt atcaaggatt tcctgcgcct gacgcaggtc ttttttgacc
     7561 ttgctgcgcg cattccacgg tacctgtacc atccagtcga tataaccacg cactacggtc
     7621 gcttctgccg acatcggaga catcattttc agcttctgca actctgcttc cgctttctct
     7681 tttgcttctt tcggcatctt cgccgcgtcg attttgcgct tcagggcttc gttttcgtcc
     7741 ggcgcgtcgt ccatttcacc gagttctttc tgaatagctt tcatttgctc gttcagatag
     7801 tactcacgct gggatttctc catctgcttt ttaacgcggt tgcgaatgcg tttctcaacc
     7861 tgcagcagat cgatttccga ttccatcatt gccatcagat attccagacg ttcgttaacg
     7921 tcggacatct ccagaacgga ctgtttgtca gccagtttca gcggcatatg tgcagcaatg
     7981 gtatccgcca gacgcgccgg atcgtcgatg ctattcaacg acgtcagcac ttctggtggg
     8041 atttttttgt tcagcttgat gtagccttcg aactggctga ttgcagtacg caccagcact
     8101 tcctgttctc gctcatcaat ggtcggcgac tccagatact ccgccttcgc agaaaagtgt
     8161 tcgccattgt cagagagcgc agaaatacgc gcgcgctgta acccctcgac cagcactttg
     8221 acggtgccgt caggcagctt cagcatctgc aatatagagg ccacggtccc gacggtgaaa
     8281 agatcgttta cacccggctc atccgttgaa gcttctttct gcgcgaccag cataattttt
     8341 ttatcatggt ccatcgccgc ttccagacaa cggatagatt tttcccgccc gacaaataag
     8401 gggatgacca tgtgcggata aaccaccaca tcgcgcagcg gcaatacggg gatttcaatg
     8461 cgttcagaac gctcaggatt catagagctc tctcttagtt taatttccgc caggtaatca
     8521 gatgacacga ctgtgcttca cgccatctat taacatgtac gtcagtatat ggggatgttt
     8581 cccccacatt caacgccgag aatagaggaa aaattaaagg ggagataaaa tccccccttt
     8641 ttggttaact aattgtatgg gaatggttaa ttattcacca gatgcctgtt gcgcttccgg
     8701 cttgccataa atcagcaacg gtttgctttg accatcaatt accgactcgt cgataaccac
     8761 tttttcgacg tcttccatgg acggcagatc gtacatggta tcgagcagtg cggcttctac
     8821 gatggaacgc aggccacggg caccggtttt acgcgccatc gctttcttag cgatagcatc
     8881 cagcgcctcg tcacggaatt ccagatccac gccttccaga ttaaacagcg cctgatactg
     8941 cttggtcagg gcgtttttcg gctctttgag gatctgaatc agagcttctt cgctcagttc
     9001 attcaacgtt gcgacaaccg gcagacgacc aatgaactca gggatcagac caaacttgat
     9061 cagatcttcc ggttcaacct gcgccagcag ctcgccttcg cttgctttgt cggacttcgc
     9121 ttttaccgtc gcgccaaaac caatgccgga gccggtttct acacgatggg aaatcacttt
     9181 atccagaccg gcaaacgcac cgccacaaat aaacaggatc ttagaggtat caacctgcaa
     9241 gaattcctgc tgcggatgtt tacgcccacc ttgcggtgga acagcagcta ccgtaccttc
     9301 gatcagtttc aacagtgcct gctgtacgcc ttcaccggaa acgtctcggg taatggacgg
     9361 gttgtctgac ttacgagaaa tcttgtcgat ttcatcgatg tagacaatac cacgctgtgc
     9421 tttctggaca tcgtagtcgc atttctgcaa cagcttctga atgatgtttt caacgtcttc
     9481 acccacataa ccggcttcgg tcagtgtagt cgcgtcggcc atggtgaacg gaacatccag
     9541 caggcgcgcc agcgtttcag ccagcagcgt tttaccggaa ccggtcggac cgatcagcag
     9601 aatgttactt ttgcccaact cgacgccatt gctggtatcg ccgttgcgca gacgtttgta
     9661 atggttgtat accgcgaccg ccagcacttt tttcgcctgt tcctggccga taacgtaatc
     9721 gtccaggtgg ttgcgaattt catgcggcgt cggtagcgca ctgcgttcac gatgcggtgc
     9781 aacttcttta atctcttcgc gaatgatgtc gttacataaa tcaacacatt cgtcgcagat
     9841 atacacggat ggaccggcaa tcagcttgcg cacttcatgc tggcttttgc cgcaaaaaga
     9901 gcaatacagc aatttgcctg agccatcttt gcgtttatct gtcatgggtc aaaacctctt
     9961 ctttgttctt tgtgccgcac acgacgacgc aaatgccatt ctcaggcgca agccgcttac
    10021 agcgttgtgc cgccctggat aagtatagcg gcacagttgc gcctctggca tcaattacga
    10081 tgggtcagaa tcgaatcgac cagaccgtat tccaccgctt caggggcgga aaggaagcga
    10141 tcgcgctcgg tatcacgttc aatctgttct aatgattgac ccgtatgaag cgccataagt
    10201 tcattcatac gccctttaac tttcagaatt tcacgggcat gaatttcgat atcggtcgcc
    10261 tggccctggt agccgcccaa cggttggtga atcatcacgc gcgaattcgg caggcaaaaa
    10321 cgtttacctt ttgcccctgc ggtcagcaag aaagcgccca tcgaggccgc ctggcccata
    10381 cagatggtgc tgacatcagg cttgataaac tgcatggtgt catagataga catcccggca
    10441 gtgatcaccc cgcctgggga gttaatgtac agatagatat ctttttctgg gttttccgct
    10501 tccaggaaca gcatctgcgc cacaatcagg ttagccatgt ggtcttcaac ctggccagtc
    10561 agaaaaatga cgcgttcctt aagtagacga gaatagatat caaaagagcg ctcaccgcgt
    10621 gaggtctgtt caatgaccat cggcaccagc gccatatggg gtgcaaagtt atctcgttcg
    10681 ccgctgtatg acatttccgt ctcctggata aaattgaaaa aacctgctgt accgattgta
    10741 accttatgga cggattatca gctagtccct gtgttatggg tacggcatca ccatatttca
    10801 agcataacaa tcttttgttg ttacgctaac accgaaagag gcttttcgca cggttccatg
    10861 caaaattcat gacaaaaaaa agcccaccac ctggcggtga cgggcctttg tgcgaattta
    10921 gcgcgttatg ctgcgtaaat tacgcctgct ggttcatcag ctcgttgaaa gtggtttctt
    10981 tttcagtcac tttcgctttc gccagtacag cttcaacagc ctgttcttcc agagcaacat
    11041 tgcgcatgtt gtccatcagt tctttgtttt tgctgtagaa ctcgataact tctttcggat
    11101 cttcgtacgc agaagccatc tcttcgatca ggcctttcac gcgctcttcg tcagctttca
    11161 gctcgttggt gcggataact tcgcccagca gcaggccaac aactacgcgg cgtttagcct
    11221 gttcttcgaa cagttcgcgc ggcagttcca gagcttgttt ttcgttgcca ccgaaacgct
    11281 gtgcagcctg gcgacgcaga acgtcgattt cgctgtcgat cagcgcagcc ggtacgtcga
    11341 tgtcgttagc ttttaccaga ccttcgatcg cctgagactt aacgcggtta cggatggcgc
    11401 tcttcagctc gcgctccatg tttttacgca cttcagcgcg cagaccttct acggaaccat
    11461 cttcaacgcc gaaacgtttg atgaattctg cagtcagttc cggcagttca cgctcttcaa
    11521 ctttcttcag gttgatagcg aatttcgctg ctttaccttt caggttttct gcgtggtatt
    11581 cttccgggaa ggtcacgtcg atggtgaact cttcgccagc tttgtggcct ttgataccgt
    11641 cttcaaagcc cgggatcata cgaccctggc ccatcgccag tacgaaatca gacgctttac
    11701 cgccttcgaa ctcttcgccg tctacagaac cggtgaagtc gatggttacg cggtcttctg
    11761 cttcaacagc gccgtctttt tctttccagg tcgcctgctg tttacgcaga gtatccagca
    11821 tgccgtcaac gtcagcgtcg gtcacttcaa cgatcggttt ttcaacttcg atcgcttcca
    11881 gaccctgcag ttcaacttcc ggataaactt caaactctac agagtaagta aagtcttcac
    11941 ccagcttgta ttcgcccgga acataagtcg gtgcgccagc cggattgatt ttttctttaa
    12001 tgatggcgtc aatgaagtta cggctcatca ggtcacccag aacgtcctgg cgtacagacg
    12061 cgccataacg ctgagcaacg atattcattg gcactttgcc tttgcggaag ccgtcaatac
    12121 gtactttttt cgcaacgttg accagctcgc ttttaacagc ggtctcgatg ctgtcagcag
    12181 cgatagtaat cgttacacgg cggccaaggc cttgagtggt ttcaactgaa acttgcatct
    12241 tgttacctca aaaaatcaca gtgctcggtc aactcttacc ccgcaagcac aggttgttgg
    12301 cttcgcggaa ccgggatgtt cttataatca atcacattcc ctgttaccag aatcatcccg
    12361 aaagcattca aataggacgc ggcattatag cggcatcact tttatgagtc gagaacggtt
    12421 attgcgcgct gtcgcgtatt ttttcacaat tcccgggcaa ttttcgtctg aatgctgggc
    12481 aaaaagcgca aaatccagac aaaacaaaac ggcccgcagg ccgtttttca taaaatcttt
    12541 agcaacatac tggaaaagcg ccgacagttg caaatgcgtt tttacgcgat gcttcctgct
    12601 ccacgacagg gaggagaggc aaagacggtg tcctgcaacc cttcccactc cttaatagtg
    12661 taagtatgca gagccagcgc atgaacggta gtagagagtt cctccgctaa agtactgtaa
    12721 atcattcgat gacgattcag aaaacgttca cccgtaaaac gatcgctgac cagcacaact
    12781 ttaaaatggc tttcagagcc ggctgggaca ttgtgacgat agctttcatc cactacttcg
    12841 aggaatacgg gttggaacgc cgcccttaat ttttcttcta tccgctcacg tatcatcatg
    12901 aaatatctcc tccgacaacg ctgagatgtc atccatccct ttaaatacta gccgctttta
    12961 ccgtttccat tgcagcttaa caacaaatat ttaggtttcg tcctggtttt tccgtcaagc
    13021 gtatgaagtt tactgattta aagattgaaa cacaggcctc gcatcagtgt ttcttttacc
    13081 aggggcgatt atttcgtcaa catgatgaat ttacatgcct tacccacttc ccctcgccgt
    13141 tggcaatgtt atgatggcgg gaatttttac caaacacgct taagagtctc tgagagcccg
    13201 aacatgttca aaaaaatcct cttcccgtta gttgctctgt ttatgcttgc aggatgcgca
    13261 aaaccgccaa caactattga agtttccccg acgattacac tgccacagca ggatccaagc
    13321 ctgatgggcg tcaccgtaag cattaatggt gccgatcagc gtaccgatca ggcgctggca
    13381 aaagtcaccc gcgataatca aatcgttacc ctgaccgcct cccgcgatct gcgtttcctg
    13441 ctgcaagaag tgctggaaaa acagatgacc gcgcgtggtt acatggttgg tccgaatggc
    13501 ccggttaatc tgcaaatcat cgttagccaa ctgtatgctg acgtgtccca gggcaacgtg
    13561 cgctacaaca tcgcgaccaa agcggatatc gccatcatcg ctaccgcgca gaatggcaac
    13621 aaaatgacca aaaactatcg tgccagctac aacgttgaag gtgcgttcca ggcctccaac
    13681 aaaaatatcg ccgatgcggt taacagtgtg ctgaccgata ccatcgctga tatgtctcag
    13741 gacaccagca tccacgaatt catcaagcag aacgcgcgtt aatttctacc ctctggcccg
    13801 gtgcaagccg ggcctgtaga cgcccatgtc cagtcaatat ttacgtattt ttcaacagcc
    13861 gcgttcagcc atattgctga tcctgggttt tgcttccggg ctaccgctcg ccctgacatc
    13921 cggtacctta caggcctgga tgacggtcga gaatattgat ctcaaaacca ttggtttctt
    13981 ctctctggta ggccaggctt acgtttttaa attcctctgg tcaccgctga tggaccgcta
    14041 cacgccccca ttttttgggc ggcggcgcgg ttggctgctc gccacgcaaa tcctgttatt
    14101 agtcgccatt gcggcgatgg gttttctcga accaggcacc caactccgct ggatggcggc
    14161 gctggcggtg gtgatcgctt tttgctcggc ctctcaggat attgtcttcg atgcgtggaa
    14221 aaccgatgtg ctcccggcag aagagcgtgg tgcgggcgcg gcaatcagcg tgctgggtta
    14281 ccgtttaggg atgctggttt ccggcggcct agccctgtgg ctggcagata aatggctggg
    14341 ctggcagggc atgtactggt tgatggcggc gctgttgatc ccctgtatta tcgcgacgtt
    14401 gcttgcacca gaaccaaccg acaccattcc tgtgcccaaa acgctggaac aagcggttgt
    14461 tgcacctctg cgagatttct ttggtcgcaa taatgcctgg cttattttgc ttcttatcgt
    14521 gctgtataag ctgggcgacg cattcgccat gagcctgaca accacgtttt tgattcgcgg
    14581 cgtcgggttt gatgcgggtg aagtaggcgt ggtaaacaaa acgcttggct tactggcgac
    14641 cattgtgggc gcattgtacg gtgggatttt gatgcagcgc ctgtcgctgt tccgggcgct
    14701 gctgattttc ggcattttac aaggtgcgtc taacgctggt tactggctgc tgtcgattac
    14761 tgataagcat ctctacagca tgggcgcagc cgtctttttc gaaaacctct gtggcgggat
    14821 gggcacatca gcctttgtcg cgctgttaat gacgctgtgt aataagtcat tttccgctac
    14881 tcaatttgcc ctgctctcag cgctttccgc tgtaggacga gtttatgtcg gccccgtggc
    14941 gggttggttt gttgaagcac acggctggtc gacattctat ctattctccg tcgccgctgc
    15001 cgtaccgggg cttattttgc tgctggtttg ccgccagacg cttgaatata cacgagtaaa
    15061 tgacaacttt atctcccgta ccgaatatcc ggcaggttat gcctttgcca tgtggacact
    15121 ggcggcgggc gtcagcctgt tggccgtgtg gttactgctg ttgacgatgg acgcgctgga
    15181 tttgacgcac ttctctttcc tgcctgctct gctggaagtc ggggttttag tcgccctttc
    15241 tggcgtcgtg cttggtggtt tgctggatta tctggcgcta cgaaaaacgc atctgacgta
    15301 atctgtaaat attatttagc tgtagtaatc actcgccgta attattacgg ctaaataaac
    15361 atcagtatcg cttaataata tttgttcttt tgtgcggctt agcgtttgga aattattgac
    15421 gccatttata aattctattt ttcttacacg attcagctaa tgagtcttta ttttctcatc
    15481 acccagttgt cactccaatg ataattattt gttaaataat tgttttattt cgcattggtt
    15541 ataccaattg cccgcccaga ctccttctgc actccccttt tgttataacg cccttttgca
    15601 acagcttctt aaaatcaacc tgatatgttt tgcaacatat gtgacctgac agccaaatcc
    15661 aagtaacagg aatttaatca tgtttacagt aatgtaacct tcccgtaaaa tgcccacaca
    15721 ctttaaacgc caccagatcc cgtggaattg aggtcgttaa atgagactca ggaaatacaa
    15781 taaaagtttg ggatggttgt cattatttgc aggcactgta ttgctcagtg gctgtaattc
    15841 tgcgctgtta gatcccaaag gacagattgg tctggagcaa cgttcactga tactgacggc
    15901 atttggcctg atgttgattg tcgttattcc cgcaatcttg atggctgttg gtttcgcctg
    15961 gaagtaccgt gcgagcaata aagatgctaa gtacagcccg aactggtcac actccaataa
    16021 agtggaagct gtggtctgga cggtacctat cttaatcatc atcttccttg cggtactgac
    16081 ctggaaaacc actcacgctc ttgagcctag caagccgctg gcacacgacg agaagcccat
    16141 taccatcgaa gtggtttcca tggactggaa atggttcttc atctacccgg aacagggcat
    16201 tgctaccgtg aatgaaatcg ctttcccggc gaacactccg gtgtacttca aagtgacctc
    16261 caactccgtg atgaactcct tcttcattcc gcgtctgggt agccagattt atgccatggc
    16321 cggtatgcag actcgcctgc atctgatcgc caacgaaccc ggtacttatg acggtatctc
    16381 cgccagctac agcggcccgg gcttctcagg catgaagttc aaagctattg caacaccgga
    16441 tcgcgccgca ttcgaccaat gggtcgcaaa agcgaaacag tcgccgaact ccatgtctga
    16501 catggcagcg ttcgaaaaac tggccgcgcc tagcgaatac aaccaggtgg aatatttctc
    16561 caacgtgaaa ccagacttgt ttgccgatgt gattaacaag tttatggctc acggtaagag
    16621 catggacatg acccagccag aaggtgagca cagcgcacac gaaggtatgg aaggcatgga
    16681 catgagccac gcggaatccg cccattaaag gggttgagga agaataaaga tgttcggaaa
    16741 attatcactt gatgcagtcc cgttccatga acctatcgtc atggttacga tcgctggcat
    16801 tattttggga ggtctggcgc tcgttggcct gatcacttac ttcggtaagt ggacctacct
    16861 gtggaaagag tggctgacct ccgtcgacca taaacgcctc ggtatcatgt atatcatcgt
    16921 ggcgattgtg atgttgctgc gtggttttgc tgacgccatt atgatgcgta gccagcaggc
    16981 tcttgcctcg gcgggcgaag cgggcttcct gccacctcac cactacgatc agatctttac
    17041 cgcgcacggc gtgattatga tcttcttcgt ggcgatgcct ttcgttatcg gtctgatgaa
    17101 cctggtggtt ccgctgcaga ttggcgcgcg tgacgttgcg ttcccgttcc tcaacaactt
    17161 aagcttctgg tttaccgttg ttggtgtgat tctggttaac gtttctctcg gcgtgggcga
    17221 atttgcgcag accggctggc tggcctatcc accgctatcg ggaatagagt acagtccggg
    17281 agtcggtgtc gattactgga tatggagtct ccagctatcc ggtataggta cgacgcttac
    17341 cggtatcaac ttcttcgtta ccattctgaa gatgcgcgca ccgggcatga ccatgttcaa
    17401 gatgccagta tttacctggg catcactgtg cgcgaacgta ctgattattg cttccttccc
    17461 aattctgacg gttaccgtcg cgttgttgac cctggatcgc tatctgggca cccatttctt
    17521 taccaacgat atgggtggca acatgatgat gtatatcaac ctgatttggg cctggggcca
    17581 cccggaagtt tacatcctga tcctgcctgt tttcggtgtg ttctccgaaa ttgcggcaac
    17641 cttctcgcgt aaacgtctgt ttggttatac ctcgctggta tgggcaaccg tctgtatcac
    17701 cgtgctgtcg ttcatcgtct ggctgcacca cttctttacg atgggtgcgg gcgcgaacgt
    17761 aaacgccttc tttggtatca ccaccatgat tatcgccatc ccgaccgggg tgaagatctt
    17821 caactggctg ttcaccatgt atcagggccg catcgtgttc cattctgcga tgctgtggac
    17881 tatcggtttt atcgtcacct tctcggtggg cggtatgact ggcgtgctgc tggcagtacc
    17941 gggcgcggac ttcgttctgc ataacagtct gttcctgatt gcgcacttcc ataacgtgat
    18001 catcggcggc gtggtcttcg gctgcttcgc agggatgacc tactggtggc ctaaagcgtt
    18061 cggtttcaaa ctgaacgaaa cctggggtaa acgcgcgttc tggttctgga tcatcggctt
    18121 cttcgttgcc tttatgccac tgtatgcgct gggcttcatg ggtatgaccc gccgtttgag
    18181 ccagcagatt gacccgcagt tccacaccat gctgatgatt gcagccagcg gtgcagtact
    18241 gattgcgctg ggtattctct gcctcgttat tcagatgtac gtttctattc gtgaccgcga
    18301 ccagaaccgt gacctgactg gcgacccgtg gggtggccgt acgctggagt gggcaacctc
    18361 ttccccgcct ccgttctata actttgccgt tgtgccgcac gttcacgagc gtgatgcatt
    18421 ctgggaaatg aaagagaaag gcgaagcgta caaaaagcct gaccactatg aagaaattca
    18481 tatgccgaaa aacagcggtg caggtatcgt cattgcagct ttctccacca tcttcggttt
    18541 cgccatgatc tggcatatct ggtggctggc gattgttggc ttcgcaggca tgatcatcac
    18601 ctggatcgtg aaaagcttcg acgaggacgt ggattactac gtgccggtgg cagaaatcga
    18661 aaaactggaa aaccagcatt tcgatgagat tactaaggca gggctgaaaa atggcaactg
    18721 atactttgac gcacgcgact gcccacgcgc acgaacacgg gcaccacgat gcaggcggaa
    18781 ccaaaatctt cggattttgg atctacctga tgagcgactg cattctgttc tctatcttgt
    18841 ttgctaccta tgccgttctg gtgaacggca ccgcaggcgg cccgacaggt aaggacattt
    18901 tcgaactgcc gttcgttctg gttgaaactt tcttgctgtt gttcagctcc atcacctacg
    18961 gcatggcggc tatcgccatg tacaaaaaca acaaaagcca ggttatctcc tggctggcgt
    19021 tgacctggtt gtttggcgcc ggatttatcg ggatggaaat ctacgaattc catcacctga
    19081 ttgttaacgg catgggtccg gatcgcagcg gcttcctgtc agcgttcttt gcgctggtcg
    19141 gcacgcatgg tctgcacgtc acttccggtc ttatctggat ggcggtgctg atggtacaaa
    19201 tcgctcgtcg cggcctgacc agcactaacc gtacccgcat catgtgcctg agcctgttct
    19261 ggcacttcct ggatgtggtt tggatctgtg tgttcactgt tgtttatctg atgggggcga
    19321 tgtaatgagt cattctaccg atcacagcgg cgcgtcccat ggcagcgtaa aaacctacat
    19381 gacaggcttt atcctgtcga tcattctgac ggtgattccg ttctggatgg tgatgacagg
    19441 ggctgcctct ccggccgtaa ttctgggaac aatcctggca atggcagtgg tacaggttct
    19501 ggtgcatctg gtgtgcttcc tgcacatgaa taccaaatca gatgaaggct ggaacatgac
    19561 ggcgtttgtc ttcaccgtgc taatcatcgc tatcctggtt gtaggctcca tctggattat
    19621 gtggaacctc aactacaaca tgatgatgca ctaagagcgg cggttatgat gtttaagcaa
    19681 tacctgcaag taacgaaacc aggcatcatc tttggcaacc tgatctcggt gattggggga
    19741 ttcctgctgg cctcaaaggg cagcattgat tatcccctgt ttatctacac actggttggg
    19801 gtgtcactgg ttgtggcgtc gggttgtgtg tttaacaact acatcgacag ggatatcgac
    19861 agaaagatgg aaaggacgaa gaatcgggta ctggttaaag gcctgatctc tcctgctgtc
    19921 tcgctggtgt acgccacctt gctaggcttt gctggcttta tgctgctgtg gtttggcgcg
    19981 aatccgctgg cctgctggct gggggtgatg ggctttgtcg tttatgtcgg cgtttatagc
    20041 ctgtacatga aacgccactc agtctacggc acattgattg gttcgctctc cggcgctgcg
    20101 ccgccggtga ttggctactg tgcagtaacc ggcgagttcg atagcggcgc agcgatcctg
    20161 ctggctatct tcagcctgtg gcagatgcct cactcctatg ccatcgccat tttccgcttt
    20221 aaggattatc aggcggcgaa cattccggta ttgccagtgg taaaaggcat ttcggtggcg
    20281 aagaatcaca tcacgctgta tatcatcgcc tttgccgttg ccacgctgat gctctctctt
    20341 ggcggttacg ctggatataa atatctggtg gtcgccgcgg cggttagcgt ctggtggtta
    20401 ggtatggctc tgcgcggtta taaagttgct gatgacagaa tctgggcgcg caagctgttc
    20461 ggcttctcta tcatcgccat cactgccctc tcggtgatga tgtccgttga ttttatggta
    20521 ccggactcgc atacgctgct ggctgctgtg tgataacaaa acctctcagt taaaaaggtg
    20581 ctacggcacc ttttttctta gcattagaaa catatccctc tcgaaatatt tactaaaaaa
    20641 tccgcatgtt taccccattc gtttgccgct ttacactagt cgcgaattta aaacagaggt
    20701 ggtaatgaac gattataaaa tgacgccagg tgagcggcgc gcgacctggg gtttaggaac
    20761 cgttttctcg ttgcgcatgc tgggcatgtt tatggttctg ccggttctga ccacgtatgg
    20821 catggctctg caaggtgcca gcgaagcatt aatcggtatt gccattggta tttatggtct
    20881 gactcaggcc gtttttcaga ttccgtttgg cctgctttcc gaccgcattg gtcgcaaacc
    20941 attaattgtc ggtgggctgg cggtgtttgc cgccggtagc gttatcgctg cgctttctga
    21001 ctccatctgg ggaattattc tgggccgggc gctacaaggc tccggtgcga ttgccgctgc
    21061 cgttatggcg ctgctttccg atctcacgcg cgaacaaaac cgtaccaaag caatggcgtt
    21121 tatcggcgtg agctttggca ttacctttgc cattgcgatg gtgcttggcc cgatcatcac
    21181 tcacaaactt gggctgcacg cgctgttctg gatgatcgct attctggcaa caaccggcat
    21241 tgcgttgacc atttgggttg tgcccaacag tagcactcac gtacttaatc gtgagtccgg
    21301 aatggtgaaa ggcagtttca gtaaagtgct ggcggaaccg cggctgctaa aactcaactt
    21361 tggcattatg tgtctgcaca tgcttctgat gtctacgttt gttgccctgc ccggacagct
    21421 agctgatgcg gggttcccgg cggctgaaca ctggaaggtc tatctggcga cgatgttaat
    21481 cgcctttggc tcggtcgtgc ctttcattat ctacgctgaa gttaagcgca aaatgaagca
    21541 agtctttgtc ttctgcgtcg ggttgatcgt ggttgcggaa attgtgttgt ggaacgcaca
    21601 aacgcagttc tggcaactgg tggtcggcgt gcagcttttc tttgtggcgt ttaatttgat
    21661 ggaagccctt ctgccctcac ttatcagtaa agagtcgcca gcaggttaca aaggtacggc
    21721 gatgggtgtc tactccacca gccagtttct tggcgtggcg attggcggtt cgctgggcgg
    21781 ctggattgac ggcatgtttg acggtcaggg ggtatttctc gctggcgcaa tgctggccgc
    21841 agtgtggctg gcagtcgcca gtaccatgaa agaaccgccg tatgtcagca gtttgcgcat
    21901 tgaaatcccg gcggacattg ccgcaaacga agcgttaaaa gtgcgtttgc tggaaactga
    21961 aggcgtcaaa gaagtgttga ttgcagaaga agagcattca gcttatgtga aaatcgacag
    22021 caaagtgaca aatcgctttg aggttgaaca ggcaattcgt caggcataaa aaacgcggcg
    22081 aattgcctgc tgcgctacgc ttaccaggcc tacaaagttc ttgagttaca aaccgttgta
    22141 ggccgaatca ggcgttcatg ccgcatctga catgaacaaa acgcacattg tcgcgattaa
    22201 tcgcggaagt ttttgaactg gaacggctgg ccgagatcgc caccacgtac catcgccatg
    22261 acagcctgca aatcatcacg agatttgcca gtcacacgga tctcatcgcc ctgaatttgc
    22321 gcctgcactt tcagtttgct gtctttaatc atcttgacga ttttcttctg ggtcgcgctc
    22381 tcaatgccct gtttcagttt cgcttccaca aaccaggttt taccgctatg aacgatattt
    22441 tccggtacat ccagtgaact gccttcaatg ccgcgcttca gcagcttggc acgcagaata
    22501 tccagcaact gattgacctg gaagtcggac tcgctcaaca ctttgatggt tttgctggcg
    22561 tcgttcagct caaatgaggc ttcaacgtta cggaagtcaa aacgggactc cacttcgcga
    22621 ctcgcgttat cgaccgcgtt acgtgcttcc tgaagatcaa cttcagagac aatatcgaaa
    22681 gatggcatct tttcctctcc cttcattttt gatgcgaagc ataatacctg caaagttaaa
    22741 taaccaacag tcctgcacca ggacgtatcc cttaattgtg catagtcgca acagcatgca
    22801 caattaaggg atagccttat tgaacacccg gagtggttgc gggtgaggag gaacaatgaa
    22861 aattaccgta ttgggatgcg gtgccttagg gcaattatgg cttacagcac tttgtaaaca
    22921 gggtcacgaa gttcagggct ggctgcgcgt accgcaacct tattgtagtg tgaatctggt
    22981 tgagacagat ggttcgatat ttaacgaatc gctgaccgcc aacgatcccg attttctcgc
    23041 caccagcgat ctgctcctgg tgacgctgaa agcatggcag gtttccgatg ccgtcaaaag
    23101 cctcgcatcc acactgcctg tagctacgcc aatactgtta attcacaacg gcatgggcac
    23161 catcgaagaa ttgcaaaaca ttcagcagcc attactgatg ggcaccacca cccatgccgc
    23221 ccgccgcgac ggcaatgtca ttattcatgt ggcaaacgga atcacgcata tcggtccggc
    23281 acggcaacag gatggcgatt acagttatct ggcggatatt ttgcaaaccg tgttgcctga
    23341 cgttgcctgg cataacaata ttcgcgccga gctgtggcgc aagctggccg tcaactgtgt
    23401 gattaatcca ctgaccgcca tctggaattg cccgaatggt gaattacgtc atcatccgca
    23461 agaaattatg cagatatgcg aagaagtcgc ggcagtgatc gagcgcgaag ggcatcatac
    23521 ttcagcagaa gatttgcgtg attacgtgat gcaggtgatt gatgccacag cggaaaatat
    23581 ctcgtcgatg ttgcaggata tccgcgcgct gcgccacacc gaaatcgact atatcaatgg
    23641 ttttctctta cgccgcgccc gcgcgcatgg gattgccgta ccggaaaaca cccgcctgtt
    23701 tgaaatggta aaaagaaagg agagtgaata tgagcgcatc ggcactggtt tgcctcgccc
    23761 ctggtagtga agagactgaa gccgtcacca ctatcgatct gctggttcgc ggcggtatca
    23821 aagtcaccac tgccagcgtc gccagcgatg gcaacctggc cattacctgc tcgcgcggcg
    23881 tgaagctgct ggcggatgcg ccgctggtcg aggtggctga tggcgaatat gacgtgatcg
    23941 tactgcctgg tggcattaaa ggcgcagagt gttttcgcga tagtaccctg ctggttgaaa
    24001 ccgttaaaca gttccaccgt tccgggcgta tcgtcgcggc tatttgcgcc gcgccagcca
    24061 ccgtgctggt gccgcacgat atcttcccga ttggtaatat gaccggcttc ccgacgctga
    24121 aagacaaaat tcccgctgaa caatggcagg acaagcgcgt cgtctgggat gcacgggtaa
    24181 aattgctgac cagccagggg ccggggacag ctatcgactt tggtctgaaa attatcgacc
    24241 tgctggttgg gcgcgagaaa gcccatgaag tggcatcaca actggtgatg gcggcaggga
    24301 tttataatta ttacgagtag tgtcggatgc ggtaagcgtc gcatctgacc agatgcgacg
    24361 ttacaaccac aaattacggg cgatatacct tcacattgtt aaagccctgc tcgcgcagat
    24421 agagcgcctg cagacggctc atcaccccgc gctcacacca cagtagccag gttctgttct
    24481 ggtcgagatc gccaaacttg gtgctcagtt tatagaacgg cagagaaact acatcaatcc
    24541 cttcgacttt cagtggctta tcttcctgtt catcgataga acggatatcg aggatcacgt
    24601 cgttcgggcc gaagccattg acggtttcca cttccaccac ttcctgctcg gtctgctggg
    24661 cgatttcgcg gatatcaacg ttattcgctt cttcaaccac tttatcgaga atgctgaagt
    24721 cgaatttctc ttcttccgct tcaatcttcg acttaaccgc tttcaccgtc gggcttttgg
    24781 agataacacc gcaatattcc ggcatcgtgc gagcaaagtc ttcggtgcca atctggcgag
    24841 ccaggttgat gatgtgctct ttgtcgtaag aaatcagcgg acgcaggatc agcgtatcgg
    24901 agacgttatc aatcaggcgc aggttggtca gcgtctgact ggacacctgg ccgagcgctt
    24961 cgccggtgac cagcgcctgt acgccgtaac gttcagccac tttagacgcg gcacgcacca
    25021 tcatacgttt gaggataacg cccatctgac cgtcgtcgat tttctcgaga atttccccga
    25081 cgaccggttc gaaattaata gcgacaaaac gcacgcggtg ggagctgcca aaacggttcc
    25141 acagataatg cgccacctga cgcacgccaa tttcatgcgc cgcgccgcca aggttaaaga
    25201 aacagtaatg cacgcggcag ccgcgacgca tcaacatata actggaaaca ccggagtcga
    25261 aaccaccgga aatgagcgac agcacatctt cctgggtgcc gatcgggaaa ccgccaatac
    25321 cttcgtagcg gcctttaatc agcaggagac gatcgtcttc cacttccaga tggacagtca
    25381 cttccggatt ggtcagcttc acgcgcgcgg actcaatatg ctgatttaaa ccgccgccga
    25441 cgtaacgttc cacatcaatc gagctaaaat catgtttgcc acggcgcttc acgcgtacgc
    25501 agaaggtttt gccttccagc tgatcgcgat actgaaccaa cgctttctca aaaatatcgt
    25561 gcatgtcggt aaacggcacg tcttcgactt cgagaatatg gtggataccc ggaatacggg
    25621 tcagggcgtc gcgaatagcc agacgctggt tttcatcttt tgcgcgaact tcgatgttat
    25681 cccagtggcg gacgacagcg agcgtctcat catagtgctt taaaacgtta cgaatgttcc
    25741 cggtaaggat ttttataaag cgcaagcgca cagattggct tttgatggtg atttccggga
    25801 acaatttaat gataaacttc atggcggcaa tggttcgttg gcaagcctta agcgacttgt
    25861 atagggaaaa atacagcagc ccacacctgc ggctgcatcc aggcgcggaa gtataccact
    25921 aacatcgctt tgctgcgcac atcaccttac cattgcgcgt tatttgctat ttgccctgag
    25981 tccgttacca tgacggggcg aaaaatattg agagtcagac attcattatg ccgaagaaaa
    26041 atgaggctcc cgccagcttt gaaaaggcgc tgagcgagct ggaacagatt gtaacccgtc
    26101 tggaaagtgg cgacctgccg ctggaagagg cgctgaacga gttcgaacgc ggcgtgcagc
    26161 tggcacgtca ggggcaggcc aaattacaac aagccgaaca gcgcgtacaa attctgctgt
    26221 ctgacaatga agacgcctct ctaacccctt ttacaccgga caatgagtaa tggactttcc
    26281 gcagcaactc gaagcctgcg ttaagcaggc caaccaggcg ctgagccgtt ttatcgcccc
    26341 actgcccttt cagaacactc ccgtggtcga aaccatgcag tatggcgcat tattaggtgg
    26401 taagcgtctg cgacccttcc tggtttatgc caccggtcat atgtttggcg ttagcacaaa
    26461 cacgctggac gcacccgctg ccgccgtaga gtgtatccac gcttactcat taattcatga
    26521 tgatttaccg gcgatggatg atgacgatct gcgtcgcggt ttgccgacct gccatgtgaa
    26581 gtttggcgaa gcaaacgcga ttctcgctgg cgacgcttta caaacgctgg cgttctcgat
    26641 tctaagcgat gccaatatgc cggaagtgtc ggatcgcgac agaatttcga tgatttctga
    26701 actggcgagc gccagcggta ttgccggaat gtgcggtggt caggcattag atttagacgc
    26761 ggaaggcaaa cacgtacctc tggacgcgct tgagcgtatt catcgtcata aaaccggcgc
    26821 attgattcgc gccgccgttc gccttggtgc attaagcgcc ggagataaag ggcgtcgtgc
    26881 tctgccagta ctcgacaagt acgcagagag catcggcctt gccttccagg ttcaggatga
    26941 catcctggat gtggtaggag atactgcaac gttgggaaaa cgccagggtg ccgaccagca
    27001 acttggtaaa agtacctacc ctgcacttct gggtcttgag caagcccgga agaaagcccg
    27061 ggatctgatc gacgatgccc gccagtcgct gaaacaactg gctgaacaat cactcgatac
    27121 ctcggcactg gaagcgctag cggactacat catccagcgt aataaataaa caataagtat
    27181 taataggccc ctgatgagtt ttgatattgc caaatacccg accctggcac tggtcgactc
    27241 cacccaggag ttacgactgt tgccgaaaga gagtttaccg aaactctgcg acgaactgcg
    27301 ccgctattta ctcgacagcg tgagccgttc cagcgggcac ttcgcctccg ggctgggcac
    27361 ggtcgaactg accgtggcgc tgcactatgt ctacaacacc ccgtttgacc aattgatttg
    27421 ggatgtgggg catcaggctt atccgcataa aattttgacc ggacgccgcg acaaaatcgg
    27481 caccatccgt cagaaaggcg gcctgcaccc gttcccgtgg cgcggcgaaa gcgaatatga
    27541 cgtattaagc gtcgggcatt catcaacctc catcagtgcc ggaattggta ttgcggttgc
    27601 tgccgagaaa gaaggcaaaa atcgccgcac cgtctgtgtc attggcgatg gcgcgattac
    27661 cgctggcatg gcgtttgaag cgatgaatca cgcgggcgat atccgtcctg atatgctggt
    27721 ggtcctcaac gacaatgaaa tgtcgatttc cgaaaatgtc ggcgcgctca ataaccatct
    27781 ggcacagctg ctttccggta agctttactc ttcgctgcgc gaaggcggga aaaaagtttt
    27841 ctctggcgtt ccgccaatta aagagctgct caaacgtacc gaagaacata ttaaaggcat
    27901 ggtagtgcct ggcacgttgt ttgaagagct gggctttaac tacatcggcc cggttgacgg
    27961 tcacgatgtg ctggggctta tcaccacgct gaagaacatg cgcgacctga aaggcccgca
    28021 gttcctgcat atcatgacca aaaaaggtcg tggttatgaa ccggcagaaa aagaccccat
    28081 cactttccac gccgtgccta aatttgatcc ctccagcggt tgtttgccga aaagtagcgg
    28141 cggtttgccg agctattcaa aaatctttgg cgactggttg tgcgaaacgg cagcgaaaga
    28201 caacaagctg atggcgatta ctccggcgat gcgtgaaggt tccggcatgg tcgagttttc
    28261 acgtaaattc ccggatcgtt acttcgacgt ggcaatcgcc gagcaacacg cggtgacctt
    28321 tgccgccggt ctggcgattg gtgggtacaa acccattgtc gcgatttact ccactttcct
    28381 gcaacgcgcc tatgatcagg tgctgcatga cgtggcgatt caaaagctcc cggtcctgtt
    28441 cgccatcgac cgcgcgggca ttgttggtgc tgacggtcaa acccatcagg gcgcttttga
    28501 cctctcttac ctgcgctgta taccggaaat ggtcattatg accccgagcg atgaaaacga
    28561 atgtcgccag atgctctata ccggctatca ctataacgac ggcccgtccg cggtgcgcta
    28621 cccgcgcggt aacgcggttg gcgtggaact gacgccgctg gaaaaactgc caattggcaa
    28681 aggcattgtg aagcgtcgtg gtgagaaact ggcgatcctt aactttggta cgctgatgcc
    28741 agaagcggcg aaagtcgccg aatcgctgaa cgccacgctg gtcgatatgc gttttgtgaa
    28801 accgcttgat gaagcgttaa ttctggaaat ggccgccagc catgaagcgc tggtcaccgt
    28861 agaagaaaac gccattatgg gcggcgcagg tagcggagtg aacgaagtgc tgatggccca
    28921 tcgtaaacca gtacccgtgc tgaacattgg tctgccggac ttctttattc cgcaaggaac
    28981 tcaggaagaa atgcgcgccg aactcggcct cgatgccgcc ggtatggaag ccaaaatcaa
    29041 ggcctggctg gcataatccc tactccactc ctgctatgct taacaaatta ttcatagact
    29101 ctaaataatt cgagttgcag gaaggcggca aacgagtgaa gccccaggag cttacataag
    29161 taagtgactg gggtgaacga atgcagccgc agcacatgca acttgaagta tgacgagtat
    29221 ggcaggagtg gcagcatgca atacaacccc ttaggaaaaa ccgaccttcg cgtttcccga
    29281 ctttgcctcg gctgtatgac ctttggcgag ccagatcgtg gtaatcacgc atggacactg
    29341 ccggaagaga gtagccgtcc catcattaaa cgcgcgctgg aaggcggcat aaatttcttt
    29401 gataccgcca atagcttttc cgatggcagc agcgaagaga tcgttggtcg cgcactgcgg
    29461 gatttcgccc gtcgtgaaga cgtggtcgtt gcgacgaaag tgttccatcg cgttggtgat
    29521 ttaccggaag gattatcccg tgcacaaatt ttgcgctcta tcgacgacag cctgcgccgt
    29581 ctcggcatgg attatgtcga tatcctgcaa attcatcgct gggattacaa cacgccgatc
    29641 gaagagacgc tggaagccct gaacgacgtg gtaaaagccg ggaaagcgcg ttatatcggc
    29701 gcgtcatcca tgcacgcttc gcagtttgct caggccctga agctacaaaa acagcacggc
    29761 tgggcgcagt ttatcagtat gcaggatcac tacaatctga tttatcgcga agaagagcgc
    29821 gagatgctgc ctctgtgtta tcaggagggc gtggcggtga ttccgtggag cccgctggcg
    29881 cgggggcgac tgacgcgtcc gtggggagaa actaccgcac gactggtgtc tgatgaggtg
    29941 gggagaaatc tctataaaga aagcgatgaa aatgacgcgc agatcgcaga gcggttaacg
    30001 ggcgtcagtg aagaactcgg tgcaacacga gcacaagttg cgctggcctg gttgttgagt
    30061 aaaccgggca ttgccgcacc gattatcggt acatcgcggg aagaacagct tgatgagctg
    30121 ctgaacgcgg tggatatcac tttgaagcca gaacagattg ccgaactgga aacgccgtat
    30181 aaaccgcatg cggtcgtagg atttaaataa gagtccaggc ctgatgagac gtgacaagcg
    30241 tcacatcagg catcggtgca caactacgac agaataccca gcggccagtg atgaccgata
    30301 aagtacagga tgcctgcgga aatcaccccg gcgacaatat cgtcgatcat gatccccatg
    30361 ccgccatgta cattgcgatc aaaccagcgg atcggccacg gcttccacat atccagaata
    30421 cggaaaatca caaacccggc ggcaacccac tgccagtcat tggtcggcag cgccatgagc
    30481 gtgatccaca taccaataaa ttcgtcccag acaatgctgc cgtgatcgtg cacgcccata
    30541 tctttcgccg tttgatgaca aagatagacg ccgatacaga tccccaacat caccaccagc
    30601 gagtagagct gccagggtaa aaaggtcatc agataccaga acggaatcgc tgccagcgat
    30661 cccatcgtgc caggaacgat tgggcttaat ccacttccga atccgacagc aagcagatgc
    30721 cacggattac tcatcttcag gcgacttttc gcgacatctt tatggcgtgg caaaatggtc
    30781 atatcctttc cagtcaaacg tgacaggttc gccgtcacga ataaaacaaa gcccttcgat
    30841 atcggcggtc atttgcccga tacaggtaaa cggtacgccc aggtgtccga gagccacatc
    30901 cagcgcgcca cggttcagtt ccggcacagt gaaacacaac tcgtaatctt caccgccaga
    30961 gagcgcccag cgcagcgcct gttccggttc aacatggcga gaaagcgcat cagaaaacgg
    31021 cagcaatgcc aggtcaatac gtgcgccgca gtcgctggct ttcacgatat gcccgagatc
    31081 ggaaatcaga ccgtcagaga gatcgatagc tgaatttgcc agatcgcgca gtgcctgtcc
    31141 ctgtaaaata cgcggcgatg gacggagatg acgtttgatc aagtagtccg catctttagc
    31201 atcggcaacc tgcaaacggt tttgcaaaat cgccagcccg gcggcgctat cgcccggtgt
    31261 accggtcaca tagatccagt cacccggttt cgctccagag cgcgttaagg ctcgtcccat
    31321 cggaacaaag ccgtggatac ccaacgtcat tgataatggc ccacgcgtgg tatcgccgcc
    31381 aatgagttgc atatcgtaat aattgagaag atcaaacaaa ctgtcgctga aggactcaag
    31441 ccacgcttcg tctacgtccg gtaaggttaa tgccagcgtc agccaggccg gatcggcccc
    31501 catcgctgcc agatcgctta ggttcaccgc cagtgcttta tacgccagat cagcaggatc
    31561 gatatcatgg aggaagtggt tgcccgccac cagcgtatca gtgctgatcg ccagggtctg
    31621 tttctcgggg atattgagaa gtgcgcaatc gtcgccgatg cccagttcga catcaagacg
    31681 agaacttctt acacggtcaa aataacgggc aatcagggag aactcgccac atgccatacg
    31741 ttatgcctca gcaggtaaaa aagaaaaggc cggcgatcat ggaacggtct tccgtgaatc
    31801 taccggcctg gatattactt tttgttaggg cgaatcacag gtgctgcttt atcgagtacg
    31861 ccgttgacga acttatggct gtcttctgcg ccgaacgatt tcgccagttc gatcgcttcg
    31921 ttaatggcca ctttgtatgg cacatcgcta cgtttagaca gctcgtacag cgcaatgcgc
    31981 agtactgctt tttctacctg tcccagttct tccagcaggc gggacaggta tggcttcatc
    32041 agtccgtcga ggtatgcggt gttagtcgcc accccggcca gcagctcacg gaagtacagg
    32101 acgtcaacat cttttacatc ctgttcagcc aggaactggt attcaacatc agcgatgtcg
    32161 ttctgggaca actgccagga gtagagcgcc tggacggcac actcacgagc gcggcgacga
    32221 gcagcaggtt tcacggattt ccccttacta atttcaggcc ttgatggctt tcaatacatt
    32281 aatcatttca agcgcggtca gtgcagcttc tgcacctttg ttgccagctt tggtgccagc
    32341 acgttcgatc gcttgttcaa tgctttcagt ggtcagaacc ccaaaagcaa ccggaatttc
    32401 gctgtcctgg gcaacatgcg ccaggccgtt gcttgcacca ccagcgacat attcaaaatg
    32461 ggcagtgcca ccacgaataa ccgtacccag cgcaatcacc gcgtcgtatt taccggtttt
    32521 agccagtgca cccgccgcca gcggcagctc ataggcacca ggcacccaaa caacggtaat
    32581 gttttcatct tttacctgac cgatacgttt cagtgcgtca attgcacctt ccagcaggct
    32641 gtcattgata aagttgttga aacgcgcgat ggtgatggcg acgcgagcgt ccggggtagc
    32701 aacgttagct tcaataatgt tcattttctt ccttcgggtt cgagtatggc cccgcagggg
    32761 ggcggatttt agcataatat ttcgtgcgct gcttcccttt cgagccggga gatcatgcac
    32821 ccactaaatg caggcaaaca tccgggccta catgacgtat ctctttgaat ttaaattggg
    32881 gggcgtcggc taatttctca agccctggca gcgtgcataa tccgcgggcg tcgctgccta
    32941 atagtttagg tgcgatatag acaatcagct catcgactaa acccgcctgc agcaatgcgc
    33001 cagcgagcgt tggccccgct tccacccaga tgctgttaat ttgctgttta cccagttgca
    33061 tcatcagtac aaccagatcc agatgacctt tatgctctgg aatcagcaag gtacgcaccg
    33121 tttccggcca ctcactagaa tcttcctgcg tacgcgcgaa ccaggtttcg ccgggctgct
    33181 gcacaatgcg atgttccggc gtcacgcgat tttggctatc aatcacaata cgtactggct
    33241 ggcggagatt ttgttgcggg tagagcgcct gagtttgttc atccagttca gaccaacgca
    33301 ctgttaaggc tggatcatcc gccagcaccg tggcgctgct ggttaaaatg gcatgacttt
    33361 gtgcgcgctg tcgttgtacg tcgcgccgcg cctgaggcga agtgatccac tggctttcgc
    33421 cgctcgccat cgccgtgcgg ccatcaagcg atgcgccaag ttttaactga atataaggaa
    33481 agccggtgcg catccgcttg agaaagcctt tattcaattg ctcggcttca ctcatcatca
    33541 gcccgtggct gacgtcaatg ccagcctgtt gcagacggta aagtccacgc ccagcgacct
    33601 gcgggttagg atcttgcatc gaggcaacca cgcgcgctac gccagcggcg atgagtgcgt
    33661 cacagcacgg tggcgtacga ccatgatggc tacagggttc gagtgtgaca taggcggtcg
    33721 cacctttggc tttttcaccc gccatacgca acgcgtgtac ttcggcatgt ggttcacccg
    33781 cacgttggtg gtaaccttca ccgacaattt cgccatcttt gacaatgacg cacccgacat
    33841 tcgggttggg atgcgtggta aaacgtcctc gttgcgccag ctttagcgcc cgcgccatgt
    33901 aatactcgtc ctgcacggct tagtcctcca ggcgcgcgat ctcttcgcca aattctttga
    33961 tatcttcgaa actgcggtag acagaggcaa aacggatata ggcgacttta tcgagctttt
    34021 tcaattgctc catcaccaga ttgccaatca tcttgctcgg cacttcgcgc tcaccggtgg
    34081 cgcgcagctg cgatttaata tgattgattg ccatttcgac gtcatcggaa ctcaccggac
    34141 gtttttccag cgcccgcagc attccgctac gcaatttctc ttcattaaac ggttcacgca
    34201 cgtcgttgct ttttacaaca cgcggcataa ccagctccgc cacttcaaag gtggtgaaac
    34261 gttcattaca caccagacac tgccgacggc ggcgtacgga tgaaccctcg cccacgagac
    34321 gagagtcaat taccttagtg tccacggcga aacagaatgg gcaatgcata cggcgtcctg
    34381 accaggtggt taacagaaat ctattttacc ctgaactgac gatacaacaa aggcgcaacg
    34441 cttttgagat aaaggatcga tgctttcgct gcttttaccc tctaccatta ggctaattgc
    34501 gacgagaaag ggacgaccta tgaatactaa cgtttttcga ctgctcctgc tgggaagcct
    34561 gttcagcctt agcgcctgtg tgcagcagag cgaagtgcga cagatgaaac acagcgtcag
    34621 cacgctgaac caagagatga cgcaactcaa taaagaaacg gtcaaaatca ctcagcaaaa
    34681 caggctgaat gcaaaatcca gcagtggggt ttaccttctg ccaggagcga aaacaccggc
    34741 aagactggaa agccagatcg gtactttacg tatgtcgctg gtgaatatta cgcctgatgc
    34801 agatggcacc accctgacgc tacgtattca gggcgagtcg aatgatccct tgcccgcatt
    34861 cagcggaact gttgaatatg ggcaaattca gggaacaata gacaactttc aggaaatcaa
    34921 tgtgcagaat caattgatta atgcccctgc cagcgtcctc gcccccagcg atgttgatat
    34981 tccgttacag ttaaagggta tttctgtaga acagttagac ttcgtacgta tccacgatat
    35041 tcaacctgtt atgcagtaaa cgtatttcgg ggcgattttg tgcgtctcgc aacatctttc
    35101 cccgtcattt tgttactccg cttacatcac cctgattgat agtaaaagtt tgcaacaagg
    35161 gtgaaagtca gtacaatccc tgcccgaatg tgtgtaaacg tgaacgcaat cgattacgta
    35221 aatgatagaa ctgtgaaacg aaacatattt ttgtgagcaa tgatttttat aataggctcc
    35281 tctgtatacg aaatatttag aaacgcaatt tgcacctttt tcactcccgc aagggatttt
    35341 caaacagtgg catacatatg aaaaaaacat tactggcagc cggtgcggta ctggcgctct
    35401 cttcgtcttt tactgtcaac gcagctgaaa acgacaaacc gcagtatctt tccgactggt
    35461 ggcaccagag cgttaacgtt gtcggaagct atcacacccg tttcggaccg cagatccgca
    35521 acgataccta ccttgagtac gaagcattcg ctaaaaaaga ctggttcgac ttctatggtt
    35581 atgcggatgc gccggtattc ttcggcggta actccgatgc taaaggtatc tggaaccacg
    35641 gttctccgct gtttatggaa atcgaaccac gtttctccat cgacaagctg accaatactg
    35701 accttagctt cggtccgttc aaagagtggt acttcgcgaa caactacatt tacgacatgg
    35761 gtcgtaataa agatggtcgc cagagcacct ggtacatggg tctgggtacc gacatcgata
    35821 ctggtctgcc gatgagcctg tccatgaacg tctatgcgaa ataccagtgg cagaactatg
    35881 gcgcagcgaa cgaaaacgag tgggacggtt accgtttcaa aattaaatac tttgtgccga
    35941 ttaccgatct gtggggcggt cagctgagct acatcggctt caccaacttc gactggggtt
    36001 ccgatttagg ggatgacagc ggtaacgcaa tcaacgatat taagacccgt actaataact
    36061 ctatcgcttc cagccatatt ctggctctga actacgatca ctggcactac tctgtcgtag
    36121 ctcgttactg gcacgacggt ggtcagtgga acgacgatgc agaactgaac ttcggcaacg
    36181 gcaacttcaa cgttcgctct accggctggg gtggttacct ggttgtaggt tacaacttct
    36241 gattatgaaa atgccgggat ttattcccgg catttcagat tgttaacaac gtgcgctttg
    36301 ttcatgccgg atgcggcgta aacgctttgt ccgacccaca aaagcacgta aattcaatat
    36361 attgctgaaa taacgtagtc ctgataagcg tagcgcatca ggcaatttta cgttttaatc
    36421 acttcttctt gttcatcatc gctttcaggt cagcgaatgg gttgtacttc gcttcaccga
    36481 catctttctg cgcatcttcc cctgcgataa cggtcgtacc atactgatcc gcttcggtat
    36541 atttcgaatg ctcatgatcg tggcaataga gacaaagcaa ttcccagtta ctgccatctt
    36601 ccgggttatt ggtatggtcg tgatcaatgt ggtgaacagt aagttcacgc aggttggaat
    36661 aaacaaactc gcgggaacag cgaccgcaga cccacggata tatttttaat gctttttcgc
    36721 gatagccgct ttctaaccgc gcatagtttt ttgggatgat agccatgaag agttacctgg
    36781 atgaaatgta gggttacgcg cagtttccca agatgcgggg attttcgcaa tctccataca
    36841 tcaaaaaaaa tcccgatctt ctgaccggga ttttcaacat caacgggaac ttgattacgg
    36901 cagaattgac ggctgatccg ccccttcttt ttccactttc tgctgcaaca tgtgttcgcg
    36961 cttcataccc agtttcagag ccaacgcaga tgccacatag atggaagatg cagtaccgat
    37021 ggaaacaccg ataagcatgg tcagcgagaa gccttccagt accggaccac cgaagaggta
    37081 cagcatcagg ataaccatca aggtagtacc ggatgtgatc aaggtacggt gcagcgtctg
    37141 ggtcaaggac acgttaaaga tttcgtaagg cgtaccgcga cggatcttgc ggaagttttc
    37201 acgaatacgg tccgatacca cgatactgtc gttaagcgag taaccgataa ccgacatcaa
    37261 cgatgccaca atggtcaggt caatctcgat atggaataac gacaaaatac ccagcgtaat
    37321 gataacgtcg tgcgccagcg caataacaac ccctgccgcc agtcgccact caaagcgaaa
    37381 acctacgtac acaaggatag acagcagcgc cgccatcaac gccatcgcac cggtttgcgc
    37441 aaggtctgcc cccacgctcg gaccgacgaa ctcaatacgc ttcactgctg cattctgatt
    37501 ggtggattcg ttaatcacct tcagaacctg gctgcccagc acctgaccgc cggtttcgcc
    37561 ttcagcaggc ggcatacgga ccatgatgtc gtggctgcta ccaaagtttt gcagcattgg
    37621 ctcttcaaaa ccggcttttt gcaatgcatc acgcattacg tcaatttcag ccggtttttc
    37681 gagcgtaatt tcgataaccg taccaccggt gaaatccagc ccccagttaa agccgcgcac
    37741 gcccataata acgatagcgg cgattaacag cagaccagag atgccaaaag cccagtagtc
    37801 ccagcgcata aagtcataga ctttacggcc gtggtttagt tgttcaacag tatattcctg
    37861 tgccacatcg cactcctcag attgacagct tcttgacgcg cttgccgcca tatagcaggt
    37921 ttacgatggc acgcgtaccg acaatcgcgg taaacatcga cgtcgccaca ccgataccgg
    37981 tagtaatcgc gaacccttta attgccccgg tacccactgc gtacaggatg atgactttaa
    38041 tcagcgtggt gatgttcgca tcgaagatag aactgaatgc gccacgataa ccttcatcaa
    38101 ttgcctgttg aacagtacgt ccgttgctca actcttcttt aatacgttcg ttgatcagta
    38161 cgttcgcatc gaccgccacc gcaagggtta agacgatacc cgcgatacct ggcatactca
    38221 gcgttgcgcc tggcaacagc gacataatgc cgacgattaa gatcaagttg gcaatcagag
    38281 cactggtcgc aatcagacca aacttcttat agaagatgat catgaacaga atagaaacca
    38341 gcagaccggc aaggcaagcc tccagcccct gttcaatgtt ctgcataccc agggttgggc
    38401 caatggtacg ttcttcaaca atctgaatcg gcgcgatcaa cgcaccggca cgcagcagca
    38461 gtgacagctg acgggcttcg ttcgggttgt tgatgccggt gatacggaag ctgttaccca
    38521 gacgagactg gatgttggcg atgttaatca cctcttcctg tttcaccaga accgcacgac
    38581 catttgcatc tttcttaccg ctgtctttgt actccacaaa cagggttgcc atcggtttgc
    38641 cgatgttgtc cttagtgaag ttagacatga tgttaccacc agcgctatcg agcgagatgt
    38701 taacctgcgg ctggttgtat tcgtcctggc tggaagtgga gtcggtgata tggtcaccgg
    38761 tcagaattac gcgtttgtac agcacaactg gctgaccttc gcgggtctgt ttcacttcag
    38821 agtcgcccgg tacgcgaccg gatgccgcag cggcctggtc aacgttggtg tttaccagac
    38881 ggaattccag cgttgccgtc gcacccagaa tctctttcgc acgcgcagtg tcctgaatac
    38941 ctggcagttc aacaacgata cggtcagcac cctgacgctg aaccaccggc tccgccacgc
    39001 caagttggtt tacacggtta cgcaggatat tgatgttctg ctgcaccgca tattcacgcg
    39061 cttcactcag acgagcatcg ctcattaccg cacgcagctg gttgctgccc tggctactaa
    39121 tcaccaggtc cggatggcgc ttgctcagat acgcaatggc ttcatcacga gctttagcat
    39181 cgcggaaagt aatgctcagg ccgtagttgt tttctttacg aacggtggta tacgggatgc
    39241 ccttttcacg cagatcgctg cgcaggctat cgatattttg ttcctggagt ttgccaagcg
    39301 cggtgtccat atccacttcc atcaggaagt gaacgccgcc acgcaggtca aggccgagct
    39361 tcatcggctc agcgtgaata gctgccagcc agcgcggcgt tgccggggca aggttaagcg
    39421 ccacgacgta tttgtcaccc ataacgccca ttaatgcttc acgagcgcgc agctgggtgt
    39481 cagtggagtc gaagcgcgca agaatagcgc cctcttccag tgccacagac ttagcagtta
    39541 ttttttcttc ttgtaacgtt ttctggacct ggatcagcgt ttgctcactg gcggcgactc
    39601 cgcgcgcacc agtgatctga acagccggat cctcaccaaa caggttggga agcgcataca
    39661 gcagaccgat gacaatcacc acgatcagca tgacgtactt ccacaaagga taacggttta
    39721 acacggcaat tcccttaggg aaaaatttta attacagcgc cttcatggtg cctttcggca
    39781 ggacggcagc tacgaagtca cgtttaataa ctacttcagt ggtgtcattc agcgcgatag
    39841 caatgtagcc gttttccgct actttggtta cgcgaccaac caggccaccg ttcgtcagaa
    39901 cttcatcacc tttggcaatg gagtccatca gctttttgtg ttctttggtg cgcttctgct
    39961 gtggacgcag gatcatgaaa tagaaaatca gaccgaatac caccagcatc aaaatcaaag
    40021 acatcgggct accttgcgcc ggtgcacccg ttgccgctac cgcatcagaa ataaaaaagc
    40081 tcattaaatt tccctcatta ttaatattaa tcaacgttca aaggtggtac ttctcgcccc
    40141 tgacgctggt aaaaatcagt tacgaagctc tctaatttac cctcttcaat agccttgcgt
    40201 aaacccgcca tcaaacgctg gtagtaacga aggttatgaa tggtgttgag tcgcgcgcct
    40261 aatatttcgt tgcaacggtc aagatgatgc aagtaagcgc gtgaataatt gcgacaggtg
    40321 tagcaatcac actcaggatc gagtgggcca gtatcgctct tatacttcgc attgcggatt
    40381 ttcaccacgc catcggtcac gaacaaatga ccatttcggg cgttgcgggt tggcattacg
    40441 cagtcaaaca tatcgatacc acgacgtacg ccttcaacca ggtcttctgg tttaccaacg
    40501 cccatcaggt aacgcggttt gtctgccgga atttgcgggc atacatgctc cagaatgcgg
    40561 tgcatatctg ctttcggctc acccacagcc agaccgccga cagcgtagcc atcaaaaccg
    40621 atatctacca gacctttaac agaaatatca cgtaaatctt cgtaaacgct gccctgaatg
    40681 ataccaaaca gcgcattttt gtttccgaga ctgtcaaaac gctcacggct acgcttcgcc
    40741 caacgcagag acatctccat ggagcgtttt gcgtaatccc agtcagcagg atacggcgta
    40801 cactcatcaa agatcatgac gatatccgaa ccaagatcgt actgaatctc cattgatttt
    40861 tcaggatcga ggaaaatcgg atcgccgttg atcgggttac ggaagtgcac gccctgttcg
    40921 gtgattttac gaatatcgcc aaggctgaag acctggaagc cgccggagtc ggtgaggatc
    40981 ggccccttcc actgcataaa atcgtgcaga tcgccgtgca gtttcatgat ttcctggccc
    41041 gggcgcagcc acaggtggaa ggtgttgccg aggataattt gcgcgccagt ggcttcaact
    41101 tcttccggcg tcatcccttt tacggtgccg taggtgccaa caggcataaa acaaggcgtt
    41161 tccactacgc cacgatcaaa gaccaggcgg ccacggcgtg cgcgaccgtc ggtggtgtcc
    41221 agttcaaatt tcattttttc tccactacgt cagaaaaaca gtccaacgtt ttaaaccagc
    41281 gccgcggaat tactccccga cgcgctcatt aattgcctgc ggattgtacg tgataaacat
    41341 cgcatcaccg taactaaaaa agcgatattt ctcttctacc gctgctttat aggcgttcat
    41401 ggtgtgttga taaccggcaa aggccgaaac cagcataatc agcgtcgact ctggcaagtg
    41461 gaagttcgtc accagcgcat cgaccacttt gtactggaag cccggataga taaagatttg
    41521 ggtatcgtcg aagaacggtt caatgagatc gtttttcgct gcctgagccg cgctttccag
    41581 cgaacgtact gaagtggtgc caaccgcaat cacccggtta ccgcgcgctt tcgccgccag
    41641 taccgcgtct accacatcct gcggtacttc agcgtattcc gagtgcatga tgtgatcttc
    41701 aatggtgtcg acgcgcaccg gctggaaggt gcccgcacca acgtgcaacg tcacaaacgc
    41761 catctccacg cctttggcgc gcaatttttc cagcaaaggc tcgtcaaaat gcagaccagc
    41821 ggtcggggct gcaaccgcgc ccggtttttc gctataaacg gtttgataaa gttcgcggtc
    41881 agcgtcttcg tccggacggt cgatatacgg cggcagcggc atatggccga tactgttgag
    41941 gatatccagt accgaacgat catcattgaa ttcgacttca aacagtgcgc cgtggcgcgc
    42001 ggtcattgtt gcgttaatac tttcgtcatc gcccagcagc agttctgcgc caggttttgg
    42061 cgctttcgag gcgcgaatat gcgcaagaat gcgtttgtcg tcgagcatcc gttcaaccag
    42121 cacttcaatc ttgccgccgc tggctttacg cccaaacagg cgcgccggga tcacgcgggt
    42181 attattaaaa accagaagat cgccggggtt gagcttatca agtaaatcgg tgaaagtacc
    42241 gtgcgtcagc gcgcccgtcg gcccgtccag cgacagtaaa cgacagctac tgcgttcagg
    42301 catgggatag tgggcaatca gggattcggg caattcaaag gagaaatcgg taacgcgcat
    42361 gacactgact cagactaaaa taagaggcgg gtagtctagt gccggggcgc tctccctgca
    42421 acctttaccc ccttttggat agaatataat cgatgaattt tttagctcac ctgcatttag
    42481 cccatctcgc ggaaagctcg ctttccggca atttactggc tgatttcgta cgcggaaatc
    42541 ctgaagaaag ttttccgccc gacgtcgtgg ctggcattca tatgcatcga cgtatcgacg
    42601 tattaaccga caatctgccg gaagtccgcg aagcacggga atggtttcgt agtgaaactc
    42661 gccgcgttgc gcctattacg ctggatgtca tgtgggatca ctttctttcc cgccactggt
    42721 cgcagctgtc gccggacttt ccgctacagg aatttgtctg ttatgcccgc gagcaagtga
    42781 tgacgatttt gccggactca ccgccacgtt ttatcaatct gaacaattac ttgtggtcag
    42841 agcagtggct ggtgcgctat cgcgatatgg atttcatcca gaacgtgtta aacggcatgg
    42901 caagccgccg cccacgtctg gatgccctgc gtgactcctg gtacgattta gacgctcatt
    42961 atgacgccct cgaaacccgc ttctggcagt tttatccgcg gatgatggcg caggcgtcac
    43021 acaaggcgtt ataaaacgtt agttcatcca taccgtagcc gaaatggcag gcaaagccag
    43081 aatcccgtca gtcagttgcc catgcccttc tttgcattgc cattgcacgg cattgagcaa
    43141 cggtgacgcg ggtagcacga cttcacaagc ttcgccacgg ttgattgcca ccagtacacg
    43201 ctgctgattc agcacgcgga caaataccac cacgttatct tccgcataca gcacctgaca
    43261 gccgccatga cgtagcgcct gacttttctt acgcagcgca atcattcgct ggtacagcgc
    43321 gaataacgcc gtatcctgct tttccacctg ccaagggaac ggtttacggc aaaacggatc
    43381 gtttttgcca tccagtccta cttcatcacc gtaataaatg cacggtacac caggccaggt
    43441 gaacagccag accaccgcca gcggcaggcg cgcaatatcc cgaccgagca gcgttttaaa
    43501 tcgcgcagta tcgtggctgt cgagctgatt aaacatacgt aattgttgtt gatgagaaag
    43561 ccctgcgcgg taattatcca tccaggccat acaggtttgg gcatcaattt gctgcggatc
    43621 gtaagagata tcggtattgg caagaaatcc ccacaacggg aatgtgaagc cacgatagtt
    43681 catggcggca tcttccacat cggcctgtaa ccattgccgt gcatcgccaa aatgttcgcc
    43741 gacaatatac gcttccggct gggtttcttt cgccgcttcg gtgatcccag caacgtgctg
    43801 catattattg cgcgccccac ccgcctcccc cagcatatgc accacatcca gccgccagcc
    43861 gtccatactc cacggcgctt tcagccaatg gcggacaata ctgtcttccc cgcgataaat
    43921 ttcattcacc agactttccg actgataatc cagcttcggc aagctggcat agccaagcca
    43981 gtcgagcgcc gtgccatcat cactaaacga gtaccagtcg cgccagggcg attcagggtt
    44041 gtgacaagct ccccccgtgc cacgattgtg cctgtcaaac caggcatggg aatcgccact
    44101 gtggttaaac acgccatcca gcaccagccg cattcccagc tgctgcgtat tgtggcgtaa
    44161 gcgcagcaac gccccatcac cgccaaactg cggatcgaca tggcgataat cctcggtatc
    44221 gtatttatgt acgctgggag ctttaaacac cggattgaga tacagcgccg tcacgccaag
    44281 ctttttcaga tacggcagtt tttcgcttat cccgtccaga tcgccgccat agaacgttga
    44341 tccgcccgcc tgcgccgtga ccggttcatc ccagtcacgc aagatgatct cttgtccggc
    44401 tgcatgatgg taatagacat gatcctgttc agcttcacga ggaagactac gcgcaaaacg
    44461 atcagggaag atctgataaa aaatctgatc cgcagcccat tgtgggccga tatccggtac
    44521 atcgacggca aactgctcca gtcgtgccgg cggcattcgg ctgaagccct gcggtgtaaa
    44581 ccaacgctgg cgatcgtgcc acagcagttt gaaactgtaa cgccgccggg gttgtccgct
    44641 ggagagatca atcgccgcac gccatgcggt gacgccaggc tgctgctgac tgcgctgctt
    44701 atgcatcgat actgacattt cttcgttatc gtgttctgta cgcagcataa tgcgctgcgg
    44761 tgggtcttcg cccgtcagcc acagtgtaat gagcagttga tctttgcttt gtttaacaaa
    44821 tgggggcacc ggcaggtgcc atgcatttaa catcataatt cccctgtgat gaaaatctga
    44881 tcaccttgcc atagcctaat gcaggatcac tcatcatctg aaggatattt gggggaggag
    44941 gacgggggcg gataactttc ggaatagccg gatcaggatt tcctcctgaa ccggcttaca
    45001 gaacgcaagt attattggtt ttcagccagc tgacgatcgt ggcggcgttt aaacatccag
    45061 ccaatcaaca gcacaacaat ccacgcgaaa ccgacataca gcgagatacg cgtatccggg
    45121 tgataaccaa tcaacccgat aataaagagc aggaaaatca gcccgccgat ggtcgttgct
    45181 accccacccg gcactttaaa tttcagcgcc ttaacttctt ctggcggcaa acggcgacgg
    45241 aaggcaattt gcgacagcag gatcataatc cacacccaca ccgtggcgaa ggttgccagc
    45301 gaagcgatca ccaggaagac gttttccggc atgatgtagt tcagatacac cgcaaacagc
    45361 agcgcggtag tcatcaccag caccgtaacc cacggaatac cgcgacgcga cgttttgctg
    45421 aaaattttcg gcgcgctgcc ctgctctgcc ataccgtgga gcatacggcc tacgccaaat
    45481 acatcactgt taattgccga cagcgaagca gtcagcacaa caaagttaag aatgctggcg
    45541 gcaaaggtaa tgcccatatg ctggaacgtc agcacgaacg ggctaccggc agtgccaacc
    45601 tgattccacg ggtagataga cataatgacg aacagcgtac cgacgtagaa caccagaata
    45661 cgcatcggca cggagttaat cgcacgcggt atcgatttct caggatcttt cgcttcaccg
    45721 gcggtaatcc cgataatttc gatcccaccg taagcaaaca tcaccatttg caacgacatt
    45781 accatgccaa gccagccgtt actgaagaag ccgccgttgc tccacaggtt atgaataccg
    45841 gtcggttgcc cgccgttgcc aatcccccag atgatgatgc cgaaaccggc gacaatcatg
    45901 atgatgatgg tggcgacttt aaagaacgag aaccagaatt ccagctcacc gaataccttc
    45961 acgctcatca ggtttacggc gcaaatgatc agcaccacgc tcagtaccca aatccagtgc
    46021 ggcaccgtcg ggaaccagac acccatatag ataccaaaag cggtcacatc ggcgatggcg
    46081 acaataagga tttcaaagca gtaggtccag ccggtaatgt aacctgccag cgggccgagg
    46141 ttttcctgcg cataacgcga gaaagagctg gcggccgggt tatgtaccga catttccccc
    46201 agcgcacgca taatgatata cgccgcgata ccaccgataa tataggccaa caacacgctc
    46261 ggaccggcca ttttgatggc gtctgccgaa ccgtaaaaca gcccggtgcc aattgctgaa
    46321 cccagtgcca taaagcgtat gtggcgggta cttagcccac gctttagctt gttcttactt
    46381 tccatcaatt cctaacccat caacacaata aaaaaccacg gggcatcgca gccccgtggt
    46441 taaaacaaat gttcagtgat ttagtgagcg ctggaggtca cctgacgacc tgccgcgcga
    46501 tcccagataa tggccagaac caccatcacc actgttggca ttaaccacgc cagaccttgt
    46561 tcggccagcg gtaaacgctg cgcccaggac ggtaagatat cgctgaatgc agatgctttg
    46621 atcccgtcga gaataccaaa aagcaggctg ataaacatcg gcggagcaat cacgcgggac
    46681 gaattatgcc accatgagcg tgtaaaactt aatacaacca gtgcgataca cggcggataa
    46741 atagcggtca gcaccggtac ggagatctgg atcagctggc ttaagccgag gttagaaacc
    46801 accatcgaga agccgccgag gataaacacc agcgtacgat aagagagcgg tacgtactgg
    46861 gcaaagaatt ctgcacaagc acaggtcagg ccaactgccg ttaccaggca ggcgatgaag
    46921 attaacgccg ccagcaggaa gctaccgccg ccgccaaagg tgtgctgaac gtaagcatgc
    46981 agaatagcag cgccgtttgc agactgatcg accagcgacg cgctgtctga acccagacgg
    47041 aacagcgcca ggtacagcag agtcagacca acacccgcca tcaggccagc ccagacggta
    47101 taacgggtca gtaggcgcgc ttcggtaacg ccacgagaac gcgccgcgtt aacaataacg
    47161 ataccaaaca ccattgcgcc cagcgtatcc atggtcagat agccgttgac gaagccgtta
    47221 gaaaacgcag cgttttgata agcctcagtc gccgtgctga tagaacccgc cggccagaca
    47281 atcgcggcaa cagacaggat gaccagcgcg ataattttca gcggcgcaag gaagttgccc
    47341 acggtatcca gcagcttgcc cggatagagc gaaaccagaa taacgatagc gaaatagacc
    47401 aggctgtaga taaacagcgg cagcgcggaa tcacccgtca gcggcgcaat ccccacttca
    47461 aaagaaacgg tagctgtacg cggcgtagcg aaaagcggcc ccaccgccag gtaacaaacc
    47521 gttgccagca gtacgccagc gactttaccg attggcgtgc tgaggctgtc aacaccgccg
    47581 ccaacttttg ccagcgccac taccgttaat accggcaggc caacggcagt aatgaggaag
    47641 ccgaatgccg cagtccagac gtgttcgcct gcctgtaagc cgaccattgg agggaaaata
    47701 atgttacctg cgccgacgaa caacgcaaat gtcataaagc ccagagcgat gatatcgcgc
    47761 gatcttaatt gatgggtcat aaatcatact gcctgtggat gtggtgttgt aacgttaaat
    47821 ttttgtgcca atccctgggg atgatacagc ggtatattta aacaatttta gcgggaaata
    47881 ctcccaacca ggcgcggcga agaatagttt ttcgatttac cgcgttaatg cagtcagtat
    47941 gacagcacct gaagcgcaat ttaaacgctt ataacgtttt aaagcaagga gggatctaaa
    48001 aaccagaggt atatagcgat aagaaatcac caaccagtat aaaacactat ttccgtgaaa
    48061 aagacatggc taatcatgcg aacaaaaaat caccaaccgt tgtaaaatct aacgcccgta
    48121 ttttgactgc aaagaaaata agccagcatc ggctggctta tggcaataaa agatgacaaa
    48181 ggcggattaa tcgctgtttt tggcaattaa acgttccggg ataacaaaac tgaaacgtgt
    48241 tccttttcct actgtactct caatattcag gcgactttcg tgatgattca ccgcatgttt
    48301 cacgatcgct aaccctaatc cgctaccgcc ggtttgccgg gaacgcgctt tatcaacgcg
    48361 ataaaaacgc tcggtcaggc gcggaatatg ctccggtgca atgcccggtc cgttatcttc
    48421 aacgctaaat tcggcaccgt gcggcactcg ctgccagcgt acggtgatat gcgtgccttc
    48481 cggcgtatga ttcacggcgt tatagaccag gttcgaaatc gcactgcgta gctgatcttc
    48541 gttgccagac accttgaggc cgttatctat ctcaaaggta aatgtctgtt ttttctgact
    48601 cagagtctga gcctcgcgct caacaacgcg cagcatcatc ggcacatcaa ccttttcatt
    48661 gagcaattgc gtcggtgcgg cttcaatttt cgacagcgtt agcaactgct tcaccagccc
    48721 ttccatccgc tgggtctgct cgcgcatggt gtgcaacgct ttttcgcgta ccgcgcctcc
    48781 cagcggctgc tcatccatca tctccaggta accctgtaac acggtcaatg gcgtacgtaa
    48841 ctcatggctc acgttggcaa aaaagttacg ccgcgcccct tccagttgat gcatttgcgt
    48901 gacatcacgc gccaccatca gcaactgttt gtgggtataa ggcatgacgc gaatttccag
    48961 atgccgcccg gtgttgagca ccagattgag cgggcgagaa aaatcacgcg ttttcaaata
    49021 ttgcgtaaac tccgggtaac gcagtaggtt aaggatgttc tgcccgttat cttccggcca
    49081 gcgcaaacca agaatttgtt gcgccagacc gttacaccag aaaataccgc cctcttccgt
    49141 ggtcagcacc accgcgtcgg gcagcgactc cgcgccgcta cgaaagcgtt taatcagatt
    49201 gcccagttca cggcggcgtt ttttatttcg cagctgcatc tggtgtaagc cgtatagtag
    49261 cggttcccag ctaccacgcc ccggtggcgg ggtcatactg cgatccaccc acagccacca
    49321 tgaaaggcgc aataaattcc agaaatgcca gataagcagt cctgttaccg atgccagcaa
    49381 aaaccagggc aggtaaccaa aaaatgcacc caggatgaaa gccgggaggc agcaaagtag
    49441 cagctccagc accagccttt tccacgacag ccgttccagc acgtaagata ctccagttaa
    49501 gaaatcataa gccctgctct gcgtccgatg agcaaggcgt taaaagcggg ttgaaaaacg
    49561 atatcctgta ccgcgcacgg tctgcaccat gcggtcatgc ccgccgggct ccagtgcttt
    49621 acgcaggcga cgaatgtgga catcgaccgt gcggtcttcc acatacacgt tagttcccca
    49681 gacgtggttt aacagctgct cgcggctgta cacgcgctca ggatgcgtca taaaaaagtg
    49741 cagcagttta aattctgtcg gccccatctc cagcggctct tcgcccgcca tcactcggtg
    49801 agatgtcggg tcgagactta atccctgcat ctcaatcacc tcttccaccg ccattggcga
    49861 aatacggcgc attaccgctt tgattcgcgc caccagctcc ttcggcgaaa acggcttggt
    49921 gatatagtca tccgcgccgg tttcaaggcc gcgcacgcga tcttcttctt cccctctggc
    49981 ggtcaacatc accactggaa tatcccgggt catcgactcg cgcttgaggt gtttgatgaa
    50041 ctggataccg gagccgccag gtaacatcca gtcaaggaga attaaatccg gccagggttc
    50101 attcagttga ttcacagcac tgtcataatc ttccgcttcg accggctgaa agccattttg
    50161 ttcgagcacg aagcagacca tttcgcgaat tggagcttca tcttctacga ccagaatacg
    50221 tctcgccatg atttgccctg ttgtaataaa taggttgcga tcattaatgc gacgtcatta
    50281 tgcgtcagat ttatgacaga tttatgaaaa gctcgtcgca catatcttca ggttattgat
    50341 ttccgtggcg cagaaaaaag caaatggcac atctgtttgg gtataatcgc gcccatgctt
    50401 tttcgccagg gaaccgttat gcgcatcctt cacacctcag actggcatct cggccagaac
    50461 ttctacagta aaagccgcga agctgaacat caggcttttc ttgactggct gctggagaca
    50521 gcacaaaccc atcaggtgga tgcgattatt gttgctggtg atgttttcga taccggctcg
    50581 ccgcccagtt acgcccgcac gttatacaac cgttttgttg tcaatttaca gcaaactggc
    50641 tgtcatctgg tggtactggc aggaaaccat gactcggtcg ccacgctgaa tgaatcgcgc
    50701 gatatcatgg cgttcctcaa tactaccgtg gtcgccagcg ccggacatgc gccgcaaatc
    50761 ttgcctcgtc gcgacgggac gccaggcgca gtgctgtgcc ccattccgtt tttacgtcca
    50821 cgtgacatta ttaccagcca ggcggggctt aacggtattg aaaaacagca gcatttactg
    50881 gcagcgatta ccgattatta ccaacaacac tatgccgatg cctgcaaact gcgcggcgat
    50941 cagcctctgc ccatcatcgc cacgggacat ttaacgaccg tgggggccag taaaagtgac
    51001 gccgtgcgtg acatttatat tggcacgctg gacgcgtttc cggcacaaaa ctttccacca
    51061 gccgactaca tcgcgctcgg gcatattcac cgcgcacaga ttattggcgg catggaacat
    51121 gttcgctatt gcggctcccc cattccactg agttttgatg aatgcggtaa gagtaaatat
    51181 gtccatctgg tgacattttc aaacggcaaa ttagagagcg tggaaaacct gaacgtaccg
    51241 gtaacgcaac ccatggcagt gctgaaaggc gatctggcgt cgattaccgc acagctggaa
    51301 cagtggcgcg atgtatcaca ggagccgcct gtctggctgg atatcgaaat cactactgat
    51361 gagtatctgc atgatattca gcgcaaaatc caggcattaa ccgaatcatt gcctgttgaa
    51421 gtattgctgg tacgccggag tcgtgaacag cgcgagcgtg tgttagccag ccaacagcgt
    51481 gaaaccctca gcgaactcag cgtcgaagag gtgttcaatc gccgtctggc actggaagaa
    51541 ctggatgaat cgcagcagca acgtctgcag catcttttca ccacgacgtt gcataccctc
    51601 gccggagaac acgaagcatg aaaattctca gcctgcgcct gaaaaacctg aactcattaa
    51661 aaggcgaatg gaagattgat ttcacccgcg agccgttcgc cagcaacggg ctgtttgcca
    51721 ttaccggccc aaccggtgcg gggaaaacca ccctgctgga cgccatttgt ctggcgctgt
    51781 atcatgaaac tccgcgtctc tctaacgttt cacaatcgca aaatgatctc atgacccgcg
    51841 ataccgctga atgtctggcg gaggtggagt ttgaagtgaa aggtgaagcg taccgtgcat
    51901 tctggagcca gaatcgggcg cgtaaccaac ccgacggtaa tttgcaggtg ccacgcgtag
    51961 agctggcgcg ctgcgccgac ggcaaaattc tcgccgacaa agtgaaagat aagctggaac
    52021 tgacagcgac gttaaccggg ctggattacg ggcgcttcac ccgttcgatg ctgctttcgc
    52081 aggggcaatt tgctgccttc ctgaatgcca aacccaaaga acgcgcggaa ttgctcgagg
    52141 agttaaccgg cactgaaatc tacgggcaaa tctcggcgat ggtttttgag cagcacaaat
    52201 cggcccgcac agagctggag aagctgcaag cgcaggccag cggcgtcgcg ttgctcacgc
    52261 cggaacaagt gcaatcgctg acagcgagtt tgcaggtact tactgacgaa gaaaaacagt
    52321 tacttaccgc gcagcagcaa gaacaacaat cgctaaactg gttaacgcgt caggacgaat
    52381 tgcagcaaga agccagccgc cgtcagcagg ccttgcaaca ggtgttagcc gaagaagaaa
    52441 aagcgcaacc tcaactggcg gcgcttagtc tggcacaacc ggcacgaaat cttcgtccac
    52501 actgggaacg catcgcagaa cacagcgcgg cgctggcgca tattcgccag cagattgaag
    52561 aagtaaatac tcgcttacag agcacaatgg cgcttcgcgc gagcattcgc caccacgcgg
    52621 cgaagcagtc agcagaatta cagcagcagc aacaaagcct gaatacctgg ttacaggaac
    52681 acgaccgctt ccgtcagtgg aacaacgaac tggcgggttg gcgtgcgcag ttctcccaac
    52741 aaaccagcga tcgcgagcat ctgcggcaat ggcagcaaca gttaacccat gctgagcaaa
    52801 aacttaatac gcttgcggcg atcacgttga cgttaaccgc cgatgaagtt gctagcgccc
    52861 tggcgcaaca tgccgagcaa cgcccactgc gtcagcgcct ggtcgcgctg catgggcaga
    52921 ttgttcccca acaaaaacgt ctggcgcagt tacaggtcgc tatccagaat gtcacgcaag
    52981 aacagacgca acgtaacgcc gcacttaacg aaatgcgcca gcgttataaa gaaaagacgc
    53041 agcaacttgc cgatgtgaaa accatttgcg agcaggaagc gcgcatcaaa acgctggaag
    53101 ctcaacgtgc acagttacag gcgggtcagc cttgtccact ttgtggttcc accagccacc
    53161 cggcggtcga ggcgtatcag gcgctggagc ctggtgttaa tcagtctcga ttactggcgc
    53221 tggaaaacga agttaaaaag ctcggcgaag aaggtgcggc gctgcgcggg caactggatg
    53281 cattaacgaa gcagcttcag cgcgatgaaa acgaagcgca aagcctccga caagatgagc
    53341 aagcacttac tcaacaatgg caagccgtca cggccagcct caatatcacc ttgcagccac
    53401 aggacgatat tcaaccgtgg ctggatgcac aagatgagca cgaacgccag ctgcggttac
    53461 tcagccaacg acatgaatta caagggcaga ttgccgcgca taatcagcaa attatccagt
    53521 atcaacagca aattgaacaa cgccagcagc agctcttaac ggcattggcg ggttatgcac
    53581 tgacattgcc acaggaagat gaagaagaga gctggttggc gacacgtcag caagaagcgc
    53641 agagctggca gcaacgccag aacgaattaa ccgcgctgca aaaccgtatt cagcagctga
    53701 cgccgattct ggaaacgttg ccgcaaagtg atgctctccc gcacagcgaa gaaactgtgg
    53761 cgctggataa ctggcggcag gttcatgaac aatgtctcgc attacacagc cagcagcaga
    53821 cgttacagca acaggacgtt ctggcggcgc aaagtctgca aaaagcccag gcgcagtttg
    53881 acaccgcgct acaggccagc gtctttgacg atcagcaggc gttccttgcg gcgctaatgg
    53941 atgaacaaac actaacgcag ctggaacagc tcaagcagaa tctggaaaac cagcgccgtc
    54001 aggcgcaaac gctggtcact cagacagcag aaacgctggc acagcatcaa caacaccgac
    54061 ctgacgggtt ggctctcact gtgacggtgg agcagattca gcaagagtta gcgcaaactc
    54121 accaaaagtt gcgtgaaaac accacgagtc aaggcgagat tcgccagcag ctgaagcagg
    54181 atgcagataa ccgtcagcaa caacaaacct taatgcagca aattgctcaa atgacgcagc
    54241 aggttgagga ctggggatat ctgaattcgc taataggttc caaagagggc gataaattcc
    54301 gcaagtttgc ccaggggctg acgctggata atttagtcca tctcgctaat cagcaactta
    54361 cccggctgca cgggcgctat ctgttacagc gcaaagccag cgaggcgctg gaagtcgagg
    54421 ttgttgatac ctggcaggca gatgcggtac gcgatacccg taccctttcc ggcggcgaaa
    54481 gtttcctcgt tagtctggcg ctggcgctgg cgctttcgga tctggtcagc cataaaacac
    54541 gtattgactc gctgttcctt gatgaaggtt ttggcacgct ggatagcgaa acgctggata
    54601 ccgcccttga tgcgctggat gccctgaacg ccagtggcaa aaccatcggt gtgattagcc
    54661 acgtagaagc gatgaaagag cgtattccgg tgcagatcaa agtgaaaaag atcaacggcc
    54721 tgggctacag caaactggaa agtacgtttg cagtgaaata actattcagc aggataatga
    54781 atacagaggg gcgaattatc tcttggcctt gctggtcgtt atcctgcaag ctatcacttt
    54841 attggctacg gtgattggta gccgttctgg tagttgtgat ggtggtatga aaaaagtcat
    54901 tttatctttg gctctgggca cgtttggttt ggggatggcc gaatttggca ttatgggcgt
    54961 gctcacggag ctggcgcata acgtaggaat ttcgattcct gctgccgggc atatgatctc
    55021 gtattatgcg ctgggagtgg tggtcggtgc gccaatcatc gcactctttt ccagccgcta
    55081 ctcactcaaa catatcttgt tgtttctggt ggcgttgtgc gtcattggca acgccatgtt
    55141 cacgctctct tcgtcttaca tgatgcttgc cattggtcgc ctggtttccg gctttccgca
    55201 tggcgcattt tttggcgtcg gcgcgatcgt gttatcaaaa attatcaaac ccggaaaagt
    55261 caccgccgcc gtggcgggga tggtttccgg gatgacagtc gccaatttgc tgggcattcc
    55321 gctgggaacg tatttaagtc aggaatttag ctggcgttac acctttttat tgatcgctgt
    55381 ttttaatatt gcggtgatgg catcggtcta tttttgggtg ccggatattc gcgacgaggc
    55441 gaaaggaaag ctgcgcgaac aatttcactt tttacgcagc ccggccccgt ggttaatttt
    55501 cgccgccacg atgtttggca acgcaggtgt gtttgcctgg ttcagctacg taaagccata
    55561 catgatgttt atttccggtt tttcggaaac ggcgatgacc tttattatga tgttagttgg
    55621 gctagggatg gtgttgggaa atatgctaag tggcaggatt tcaggacgtt attcaccact
    55681 gcgcattgca gcagtgactg actttataat tgtactggca ctgctgatgc tctttttctg
    55741 cggcggcatg aaaacaacgt cgcttatttt tgcttttatt tgttgcgcgg ggttatttgc
    55801 cctttcagca cctctgcaaa tattattact acaaaatgcc aaaggcggag agttattagg
    55861 tgccgcaggt gggcaaatag cgtttaacct tggtagcgcc gtcggcgcat attgcggtgg
    55921 tatgatgctg acgctggggc tggcatataa ttacgtggcg ctgcctgccg ccctgctttc
    55981 gtttgctgcg atgtcgtcat tgctgctgta tggtcgctat aagcgccagc aagcggcgga
    56041 tagtccggtg ctggcgaaac cactggggta gattgcagtc acgttgcgcg ttgcttattg
    56101 ccggatgcgg cgtgaacgcc ttatccgccc tacgcggttc cggcacattt tgtaggcctg
    56161 ataagacgcg gcaagcgtcg catcaagcat gatgcgccaa ttgcctacgt tttttactct
    56221 tgtggccata accacgcagc gccgcgtacg ccgctggaat caccgtgctt cgccttacgc
    56281 accggcgttt cacattcgcc gccgaagaca aattgtttaa tcaactgccc aaccgtttga
    56341 tataaacggt ctacattgct catcccgccc cccaggacaa tcacatccgg atcgagaata
    56401 ttcacgacat gtgccagcga ttttgccagc cgcagctcgt agcgacgcaa tgccagttcc
    56461 gctaccggat cgctttcttc aaccaggcgg ataatttcac tgcctttcag cgcatgtccg
    56521 ctcaaacgac gataatccgt cgcgaatccc gtgcccgaaa taaaggtttc aatacaacct
    56581 tgtttaccgc aataacaagg gacttcctcg cgataacgca gttcgtcttc gtccatccac
    56641 ggtagcggat tgtgtcccca ctcacctgcc gtgccattgc cgccgatatg cgcccgccca
    56701 ttgaatgcca cgcccgcgcc gcatcccgtg ccgataatca cggcaaatac tgtctgcgct
    56761 cccgctgccg cgccatctac tgcttctgaa accgccagac agttggcgtc atttgccagc
    56821 cgcacttccc gctgcaacct cgcgcttaag tctttatcga atggctgacc gttgagccag
    56881 gttgaattgg cattcttaac cacaccggtg taaggcgaaa ttgagccagg aatgcccata
    56941 cctaccgttc cgcgctgccc tgtcgcctgc tccgccatat caaccaacgt ggcgatcgtt
    57001 tcaatagtct gccggtaatc atcacgcggc gtgggcagac gatggcggta caactgctcc
    57061 cctgcatcgc ccagtgcaat cacttcagtt ttggtgccgc ctaaatcgat acctatacgc
    57121 acggtactct ccttattttt ttcaatatca atagcgtaga gacggacaac cggattggca
    57181 atgcaaggcc gccgacaatt cgttatcatg cccgctaaat ttaacgacaa ggccgtggaa
    57241 attatcatgc tgtggttcaa aaatttaatg gtttaccgtc ttagccgcga gatttcgctg
    57301 cgtgcagaag agatggaaaa acagctagcc tcgatggcat ttaccccatg cggcagccag
    57361 gacatggcga agatgggctg ggttcctccg atgggatcgc acagcgatgc gttaacgcac
    57421 gttgccaatg gtcaaattgt tatctgcgcg cgcaaagaag aaaaaatcct cccgtccccg
    57481 gtgattaaac aggcgctgga agcgaaaatc gccaaactgg aagcggaaca ggcgcgtaag
    57541 ctgaagaaaa ccgaaaaaga ttcgctgaaa gacgaagtgc tgcactctct gttgccgcgt
    57601 gctttcagcc gttttagcca gacaatgatg tggatcgaca cggttaatgg tttgattatg
    57661 gtggactgcg ccagtgccaa aaaagcggaa gatacgctgg cattactgcg taaaagcctg
    57721 gggtcgttac cggttgtacc attgagcatg gaaaacccga ttgaactgac gctgaccgaa
    57781 tgggttcgct ccggtagtgc ggcacagggc ttccagctgc ttgatgaagc cgagctgaaa
    57841 tcgttgctgg aagatggcgg cgtgatccgc gcgaagaaac aagatctgac cagcgaagag
    57901 atcaccaatc acattgaagc cggaaaagtg gtgactaaac tggcgctcga ctggcagcag
    57961 cgcattcagt ttgtgatgtg cgacgatggt tcgctcaagc gtctgaagtt ctgcgacgag
    58021 ctgcgcgatc aaaacgaaga tatcgaccgt gaagatttcg cccagcgttt tgatgccgat
    58081 ttcatcctga tgactggtga actggcagcg ttaattcaaa acctgattga aggattaggt
    58141 ggcgaagcac aacgttaatt gctgattttc ctttaatgcc ggatgcgacg cctgccgcgt
    58201 cttatccggc gtacgaagcc acaccaggca tataattatt cgctacggcg agcaataatt
    58261 tttagcgcag caatattatg ctttttacgc tgtaacttgc tccatggacg ttgtgtcatt
    58321 gtttttcctc aagccggtga cgcgcttcac tgcgaatttt ccgcgttgcc ccggttttca
    58381 ctaaatcacg taactgcgaa agatcgggtt gttcgacaaa taagagtaat gatggatcct
    58441 cttttaacca cgccgttttc acatcggcag acagctgtgg attattaata gccagcgacc
    58501 gatcctgagg ctcagtcaat gtcgttaaca acaacgccgg ggtatgtgcg ttgcgcaatg
    58561 ccgtttggcg caaatgatca ttgcgacttt gcagcaatgc acgccaccat tcatcatctg
    58621 atttatcttt tatcgtagcc agtaccgcaa aaaatacatg gagatcttca ctgatattta
    58681 tatcaacctt gatgaattat gaatataccg gaaatcgaaa aaatcgcagc aaaggaaata
    58741 ttattatggg tttatgagag gatatttcag aaaaatgccc tcccgttcaa ggggaaggcg
    58801 aggaattaca gatagcgaca cagataagag gtgggttcgg caacttgcag atgaaactcg
    58861 ctgtgaccgg gaacattaaa caccgaaccg gcttcataca cttgccagtc ggtcgcgtca
    58921 ggcagtaaca cattcagcgc gccactgatt accgtcatct cttccggctc agcggtgctg
    58981 aaggtgtatt cgccttcaac cataacaccc acactggcgc gaccagtgct gctgctggaa
    59041 aagccgatcg atttcacttt gccggaaaag tactcattac tttgaagcat aaactggccc
    59101 tgtctggtag ttgaatatct acttcactat aggggccaga ggcgctgact gtcacgcaaa
    59161 attacaccag taattccgca gccagccgtg caatcaatac gttagacagc aagacaggaa
    59221 catcgagctg tttttgcagt aaatcgcgat gacgttggtg aaatcctaaa caatccagca
    59281 tgatgacatc agcccctttt gccagtaatt ctttcccggc atcaatgatt ttttgttctg
    59341 aatcatgaat ggggttaccc aatgaaaata ccggcgattt ctgcaaaatt tgccattttt
    59401 gcgcctgaac gggcagcatc tcctcaaccg gaacgataac ccccacttga tgatcttcaa
    59461 caatagagga aaccagcggt ggcaatatcc gcgacggctc aagaaagatc gtattacgcg
    59521 cagtcatact actaatgttt gccgtactca ttaatataat gacgtcgtaa ccccgattat
    59581 cgagtacttc aaccacacct tgcaggtcac gctccacttt gcgacgcgaa acatgggcca
    59641 gctggttgtc atttaataat gtgagaatgg tgtcttcgcc tgcttctggc gcgtactccg
    59701 ccatcacttc ttcacgactt aacttcccca gcaggctatg atgggaaata ttatcttcgt
    59761 caatgtattc cgtcaggagc ggcaaaactt cctgcatagg tacaatgccg atggtcagga
    59821 tcgccaacga cgcactcatt gttcatccac cttacttttc tttcgtcgtt aattaccggg
    59881 caagtgtgaa gcaccactgc tgacacttac ttgtgctaca aatgacaaaa agcgtagcag
    59941 cagacgtgca cggcataatg taaagattcc aaatgattcc agtaatggat tgttattgtt
    60001 taatattcta attattagaa aaacatgaat tatgaaaaat gtgacgcaga tcccttgtag
    60061 tggcactcag aatcccttcc gagcagtctg atgcgatcac tctttgtcag ggtcctcata
    60121 gcgttttttc gcatccatcg cttcctgagc ggtcggatgt tcactgatca acgagtctgg
    60181 tttaggatga tcggctctga gttggtacca ggttaccgtc tgtcctggtt ttcctttttc
    60241 aatcgtcact atatatgctt cacgaggata aggcggtttc gttggcatcg ttcttcctta
    60301 tttcacggga tgaacgttaa gtataggcgc tcgaaaatca acaattgatc gtctgtgcca
    60361 gggcgctacg aatttcagaa atcacctggc tgggttcgtt tgttgcgtcg atgataatat
    60421 gcgcaacttc gcgatatagc gcatcgcgtt cttccagcac ttcctgaact tcttcgctca
    60481 gcggttttcc cgttaaggtt ggccgtaaat cttcttccgg tgcagcttgc agtcggttaa
    60541 ccaggactga tactggcgca cacaaataaa ccacgatccc gttattttgc atgaagtgac
    60601 gattaaattc cgtcagaata atgccgccgc ctgtagcgat aacggtggat ggcgcagtta
    60661 ccgcttccag cgccgccgtt tctctggcgc gaaatcccgc ccactcttcc ctttcgacga
    60721 tctccgcgac cgtcatattg agctgtgatt gcaaccactg atcggtatcg acaaaccgac
    60781 ggttaagcga atcggcaagg gccattccga ccgttgtttt accacagccc cgaggcccga
    60841 tcagaaaaag aggttgtgtc atcgtgggtt ttccccaata ggtcgcaatg cggcgaaagc
    60901 cggtgtcatg agaatagcga tcataccatc aaactagtac aatttcgatt gtaaagga
//
LOCUS       DAJOWB010000016        56271 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJOWB010000016 DAJOWB010000000
VERSION     DAJOWB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.016"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            37..2694
                     /locus_tag="OHO85_001123"
     CDS             37..2694
                     /locus_tag="OHO85_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HCQ2670536.1"
                     /translation="MTFFDFWGKTRRGEKEGGDDYHLLCWHSLDVAAMGYLMVKRNCF
                     GLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQEGARKNYEK
                     ISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGRPPDRMDELD
                     NFLPEDKAAARDFLLAIKALFPRIEIPAFWDDDEGIELIKHLSWYISATVVLADWTGS
                     STRFFPRVAQAMDIKHYWQKALVQAQNALTVFPPKAETAPFTGINTLFPFIENPTPLQ
                     QKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGLFFGLPTMATANA
                     MYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGC
                     AAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYM
                     SCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGAEGQQEAPLLGKDDYPWL
                     THVTKTDVHSHRVATRKEVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSVDDA
                     IKVYRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKEDGSQRAGKVLICTQ
                     VLEQSVDCDLDEMISDLAPIDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILA
                     PVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPHSARLLIESVYGE
                     DVVMPEGFARSEQEQVGKYYCDRARAKKFVLNFRPGYAANINDYLPEKLSTRLAEESV
                     SLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGDAFRQWCIEQRQ
                     DPEMANVILVTDDESCGYSAMEGLTGKVG"
     gene            2792..4354
                     /gene="casA"
                     /locus_tag="OHO85_001124"
     CDS             2792..4354
                     /gene="casA"
                     /locus_tag="OHO85_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HCQ2670537.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDHRRWDDIWEDGLEVEKLREALLSLEHAFQFGPDSPSFM
                     QDFEALTGDKVQVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKMWLNVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDDQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQNDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRFVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVNLERIMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            4351..4887
                     /gene="casB"
                     /locus_tag="OHO85_001125"
     CDS             4351..4887
                     /gene="casB"
                     /locus_tag="OHO85_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HCQ2670538.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            4899..5954
                     /gene="cas7e"
                     /locus_tag="OHO85_001126"
     CDS             4899..5954
                     /gene="cas7e"
                     /locus_tag="OHO85_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HCQ2670539.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWAMAEKGTEQPRSLAAAFYEPINGTRQLEVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            5965..6711
                     /gene="cas5e"
                     /locus_tag="OHO85_001127"
     CDS             5965..6711
                     /gene="cas5e"
                     /locus_tag="OHO85_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HCQ2670540.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTIQMPKEVRKARYFSRREELSDPD
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGEHDGLVASKILRRRDV
                     PLNRQQWLFGERTINQGPWLSKEEPCTSQE"
     gene            6693..7343
                     /gene="cas6e"
                     /locus_tag="OHO85_001128"
     CDS             6693..7343
                     /gene="cas6e"
                     /locus_tag="OHO85_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCQ2670541.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELRTGQQLCFNLRANPTICKS
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            7340..8263
                     /gene="cas1e"
                     /locus_tag="OHO85_001129"
     CDS             7340..8263
                     /gene="cas1e"
                     /locus_tag="OHO85_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HCQ2670542.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRGG"
     gene            8260..8553
                     /gene="cas2"
                     /locus_tag="OHO85_001130"
     CDS             8260..8553
                     /gene="cas2"
                     /locus_tag="OHO85_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCQ2670543.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     repeat_region   8650..9410
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=8650..8677
                     /rpt_unit_seq="gtgttccccgcgccagcggggataaacc"
     gene            complement(9431..10468)
                     /gene="iap"
                     /locus_tag="OHO85_001131"
     CDS             complement(9431..10468)
                     /gene="iap"
                     /locus_tag="OHO85_001131"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCQ2670544.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            10720..11628
                     /gene="cysD"
                     /locus_tag="OHO85_001132"
     CDS             10720..11628
                     /gene="cysD"
                     /locus_tag="OHO85_001132"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCQ2670545.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            11630..13057
                     /gene="cysN"
                     /locus_tag="OHO85_001133"
     CDS             11630..13057
                     /gene="cysN"
                     /locus_tag="OHO85_001133"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCQ2670546.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEKTFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMAWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFINRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            13057..13662
                     /gene="cysC"
                     /locus_tag="OHO85_001134"
     CDS             13057..13662
                     /gene="cysC"
                     /locus_tag="OHO85_001134"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCQ2670547.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            13712..14035
                     /locus_tag="OHO85_001135"
     CDS             13712..14035
                     /locus_tag="OHO85_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCQ2670548.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            14229..14540
                     /gene="ftsB"
                     /locus_tag="OHO85_001136"
     CDS             14229..14540
                     /gene="ftsB"
                     /locus_tag="OHO85_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCQ2670549.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTLVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            14559..15269
                     /gene="ispD"
                     /locus_tag="OHO85_001137"
     CDS             14559..15269
                     /gene="ispD"
                     /locus_tag="OHO85_001137"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCQ2670550.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPSDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLVRLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            15269..15748
                     /gene="ispF"
                     /locus_tag="OHO85_001138"
     CDS             15269..15748
                     /gene="ispF"
                     /locus_tag="OHO85_001138"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCQ2670551.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            15745..16794
                     /gene="truD"
                     /locus_tag="OHO85_001139"
     CDS             15745..16794
                     /gene="truD"
                     /locus_tag="OHO85_001139"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCQ2670552.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            16775..17536
                     /gene="surE"
                     /locus_tag="OHO85_001140"
     CDS             16775..17536
                     /gene="surE"
                     /locus_tag="OHO85_001140"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCQ2670553.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            17530..18156
                     /gene="pcm"
                     /locus_tag="OHO85_001141"
     CDS             17530..18156
                     /gene="pcm"
                     /locus_tag="OHO85_001141"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCQ2670554.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            18296..19435
                     /gene="nlpD"
                     /locus_tag="OHO85_001142"
     CDS             18296..19435
                     /gene="nlpD"
                     /locus_tag="OHO85_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCQ2670555.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            19498..20490
                     /gene="rpoS"
                     /locus_tag="OHO85_001143"
     CDS             19498..20490
                     /gene="rpoS"
                     /locus_tag="OHO85_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCQ2670556.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(20584..21948)
                     /locus_tag="OHO85_001144"
     CDS             complement(20584..21948)
                     /locus_tag="OHO85_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCQ2670557.1"
                     /translation="MSTITLLCIALTGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(22037..22813)
                     /locus_tag="OHO85_001145"
     CDS             complement(22037..22813)
                     /locus_tag="OHO85_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCQ2670558.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            complement(22818..23456)
                     /locus_tag="OHO85_001146"
     CDS             complement(22818..23456)
                     /locus_tag="OHO85_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCQ2670559.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(23453..24715)
                     /locus_tag="OHO85_001147"
     CDS             complement(23453..24715)
                     /locus_tag="OHO85_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HCQ2670560.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(24712..25620)
                     /locus_tag="OHO85_001148"
     CDS             complement(24712..25620)
                     /locus_tag="OHO85_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCQ2670561.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            25816..26583
                     /locus_tag="OHO85_001149"
     CDS             25816..26583
                     /locus_tag="OHO85_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCQ2670562.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(26634..27290)
                     /gene="pphB"
                     /locus_tag="OHO85_001150"
     CDS             complement(26634..27290)
                     /gene="pphB"
                     /locus_tag="OHO85_001150"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCQ2670563.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFYPETD
                     LLISTGDNIDRGPKSLNVLRLLNQPWFTSVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(27396..29957)
                     /gene="mutS"
                     /locus_tag="OHO85_001151"
     CDS             complement(27396..29957)
                     /gene="mutS"
                     /locus_tag="OHO85_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCQ2670564.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            30033..30167
                     /locus_tag="OHO85_001152"
     CDS             30033..30167
                     /locus_tag="OHO85_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670565.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            30244..30597
                     /locus_tag="OHO85_001153"
     CDS             30244..30597
                     /locus_tag="OHO85_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCQ2670566.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKTRE"
     gene            complement(30634..32712)
                     /gene="flhA"
                     /locus_tag="OHO85_001154"
     CDS             complement(30634..32712)
                     /gene="flhA"
                     /locus_tag="OHO85_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCQ2670567.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32786..33796)
                     /gene="hypE"
                     /locus_tag="OHO85_001155"
     CDS             complement(32786..33796)
                     /gene="hypE"
                     /locus_tag="OHO85_001155"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCQ2670568.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAKTARTAGIAIVTGDTKVVQRGAVDKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(33793..34914)
                     /gene="hypD"
                     /locus_tag="OHO85_001156"
     CDS             complement(33793..34914)
                     /gene="hypD"
                     /locus_tag="OHO85_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCQ2670569.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTNAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            complement(34914..35186)
                     /gene="hypC"
                     /locus_tag="OHO85_001157"
     CDS             complement(34914..35186)
                     /gene="hypC"
                     /locus_tag="OHO85_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCQ2670570.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(35177..36049)
                     /gene="hypB"
                     /locus_tag="OHO85_001158"
     CDS             complement(35177..36049)
                     /gene="hypB"
                     /locus_tag="OHO85_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCQ2670571.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIAEAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(36053..36403)
                     /gene="hypA"
                     /locus_tag="OHO85_001159"
     CDS             complement(36053..36403)
                     /gene="hypA"
                     /locus_tag="OHO85_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCQ2670572.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            36615..37076
                     /gene="hycA"
                     /locus_tag="OHO85_001160"
     CDS             36615..37076
                     /gene="hycA"
                     /locus_tag="OHO85_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCQ2670573.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            37201..37812
                     /gene="hycB"
                     /locus_tag="OHO85_001161"
     CDS             37201..37812
                     /gene="hycB"
                     /locus_tag="OHO85_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCQ2670574.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            37809..39635
                     /gene="hycC"
                     /locus_tag="OHO85_001162"
     CDS             37809..39635
                     /gene="hycC"
                     /locus_tag="OHO85_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCQ2670575.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQALAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            39638..40561
                     /gene="hycD"
                     /locus_tag="OHO85_001163"
     CDS             39638..40561
                     /gene="hycD"
                     /locus_tag="OHO85_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCQ2670576.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISSLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            40579..42288
                     /gene="hycE"
                     /locus_tag="OHO85_001164"
     CDS             40579..42288
                     /gene="hycE"
                     /locus_tag="OHO85_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCQ2670577.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            42298..42840
                     /gene="hycF"
                     /locus_tag="OHO85_001165"
     CDS             42298..42840
                     /gene="hycF"
                     /locus_tag="OHO85_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCQ2670578.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            42840..43607
                     /gene="hycG"
                     /locus_tag="OHO85_001166"
     CDS             42840..43607
                     /gene="hycG"
                     /locus_tag="OHO85_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCQ2670579.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            43604..44014
                     /gene="hycH"
                     /locus_tag="OHO85_001167"
     CDS             43604..44014
                     /gene="hycH"
                     /locus_tag="OHO85_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCQ2670580.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            44007..44477
                     /gene="hycI"
                     /locus_tag="OHO85_001168"
     CDS             44007..44477
                     /gene="hycI"
                     /locus_tag="OHO85_001168"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCQ2670581.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            44502..45263
                     /locus_tag="OHO85_001169"
     CDS             44502..45263
                     /locus_tag="OHO85_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670582.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            45437..45748
                     /locus_tag="OHO85_001170"
     CDS             45437..45748
                     /locus_tag="OHO85_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HCQ2670583.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            45745..46164
                     /locus_tag="OHO85_001171"
     CDS             45745..46164
                     /locus_tag="OHO85_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2670584.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(46278..47702)
                     /gene="ascB"
                     /locus_tag="OHO85_001172"
     CDS             complement(46278..47702)
                     /gene="ascB"
                     /locus_tag="OHO85_001172"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCQ2670585.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLISASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(47711..49168)
                     /gene="ascF"
                     /locus_tag="OHO85_001173"
     CDS             complement(47711..49168)
                     /gene="ascF"
                     /locus_tag="OHO85_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCQ2670586.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVMLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            49428..50438
                     /gene="ascG"
                     /locus_tag="OHO85_001174"
     CDS             49428..50438
                     /gene="ascG"
                     /locus_tag="OHO85_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCQ2670587.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            50587..51114
                     /gene="hydN"
                     /locus_tag="OHO85_001175"
     CDS             50587..51114
                     /gene="hydN"
                     /locus_tag="OHO85_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCQ2670588.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            51267..53519
                     /gene="hypF"
                     /locus_tag="OHO85_001176"
     CDS             51267..53519
                     /gene="hypF"
                     /locus_tag="OHO85_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCQ2670589.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LADKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(53647..54780)
                     /gene="norW"
                     /locus_tag="OHO85_001177"
     CDS             complement(53647..54780)
                     /gene="norW"
                     /locus_tag="OHO85_001177"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCQ2670590.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQAQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKVVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(54777..56216)
                     /gene="norV"
                     /locus_tag="OHO85_001178"
     CDS             complement(54777..56216)
                     /gene="norV"
                     /locus_tag="OHO85_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCQ2670591.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
BASE COUNT        13128 a        14495 c        15334 g        13314 t
ORIGIN      
        1 aaatatcctt taagtttact gaaggataaa aatattgtga ctttctttga tttctgggga
       61 aaaacccgac gcggcgagaa agaagggggc gacgactatc accttctttg ctggcattcg
      121 ctggatgtgg ccgcgatggg ctatttaatg gttaaaagaa attgcttcgg gctggctgat
      181 tattttcgtc aattagggat ttctgacaag gaacaggcgg ctcaattttt cgcctggttg
      241 ctgtgctggc acgatattgg aaaatttgcc cgctcttttc aacaacttta cctgccccct
      301 gaactcaaga ttcaggaagg tgccagaaaa aattacgaaa aaatctctca ttcaacgctg
      361 ggctactggt tgtggaatca ttatttaagt gaatgtcagg agttacttcc ttcatcttcg
      421 ctctctccgc gcaaacttag gcgagtgata gaaatgtgga tgcccgtgac gacagggcat
      481 catggtcggc cccctgatcg tatggatgaa ttggataatt ttctgcctga agacaaagct
      541 gcagcacgag attttctcct tgcaatcaag gcactgtttc cgcgaataga gatccccgca
      601 ttctgggatg atgacgaggg cattgaactt ataaaacacc tttcctggta tatctctgca
      661 accgtcgtac tcgcagactg gacaggttcg tcaacgcgat tttttccacg cgtcgcacag
      721 gcaatggata ttaaacatta ctggcagaag gctttagtgc aggctcaaaa cgcattaacc
      781 gtctttcctc caaaagcaga aaccgcacct ttcaccggaa ttaatacact gtttcctttt
      841 attgagaatc cgacaccgtt acagcaaaag gtgctggatc tggatatcag ccagccagga
      901 ccgcagttat ttattctgga agatgtgact ggcgcaggta aaacagaagc ggcgcttatc
      961 ctggcgcaca ggttgatagc tgcggggaaa gctcaggggc tgttttttgg tctgccaaca
     1021 atggcaacgg ccaatgccat gtacgatcgg ctggtcaaaa cctggcttgc tttctattcg
     1081 ccagaatccc gccccagcct ggtgctggca catagtgccc gcacattaat ggaccgcttt
     1141 aatgaatcac tctggtccgg tgatttagtc gggtcagaag aaccggatga acaaacgttc
     1201 agtcagggat gtgcggcctg gtttgccgac agtaacaaga aggcgttgct ggctgaaatt
     1261 ggcgtcggca cgctggatca ggcgatgatg gcggtgatgc cgttcaaaca taataatctg
     1321 cggcttctgg ggctgagtaa caaaatcctg ctggctgatg agatccacgc ctgtgacgct
     1381 tacatgtcgt gcattcttga agggctgatc gagcggcagg cgcgtggcgg aaacagcgtt
     1441 attttgcttt ctgctacgtt atctcaacag cagcgcgaca aactcgtcgc cgcctttgcg
     1501 cgtggtgcag agggccagca agaagcccca ctcctgggaa aagatgatta cccctggctg
     1561 acgcatgtca cgaaaaccga tgtacattcg caccgggtag cgacgcgcaa agaagttgag
     1621 cgtagcgtga gcgtgggttg gcttcatagc gaacaagaat gtattgcgcg tatcgaatca
     1681 gcggtaagtc aggggaaatg catcgcctgg atccggaatt ctgtcgatga cgctattaag
     1741 gtttatcgtc agttacttgc ccgcggcgtc attcctgctt ccagcctttc actttttcat
     1801 agccgctttg cttttagcga tcgccagcga attgaaacgg aaacgctggc acgctttggt
     1861 aaagaagacg gttcacagcg tgccggaaaa gtcctcattt gtactcaggt cttagagcag
     1921 agcgttgatt gtgacctgga cgaaatgatc tccgacctgg cccccattga tttgctaatc
     1981 cagcgagcag gacgattaca gcggcatatc cgcgatatta atggtcagtt aaagcgtgac
     2041 ggaaaagacg agcgttcccc ccctgaattg ctgattctgg ctcccgtctg ggacgactct
     2101 cctggcgacg aatggtttgg cagtgccatg cgtaacagtg catttgtcta tcccgatcat
     2161 gggcgaatct ggctgacgca gcgtgtgctg cgtgagcaag gtgctattca gatgccacac
     2221 tcagcccggc ttcttattga atccgtctac ggtgaggacg tggtaatgcc ggaaggattt
     2281 gcccgcagcg agcaggagca agtgggcaaa tattactgtg atcgcgcaag ggctaaaaag
     2341 tttgtgctga acttcaggcc cggctatgcc gccaatatca acgattacct cccggaaaaa
     2401 ctgtcgacac gtctggctga ggagtctgtt tccctgtggc tggctacctg tattgacggt
     2461 gtggtgaagc cttatgccac cggtgctcac gcatgggaaa tgagcgttgt cagagtgcgc
     2521 cgaagctggt ggaaaaaaca tcgggatgag ttttctttac tggaagggga tgcgttcagg
     2581 cagtggtgca ttgaacagcg gcaagatccg gagatggcaa acgtgatttt agtcaccgat
     2641 gacgaaagtt gcgggtattc ggctatggaa ggattgactg gcaaggttgg ttgagatgcc
     2701 tgcgacaggg ctctctgaat agttgataca ccgaaaagga tggaatgatg gacaaccgac
     2761 ctgggatggt cttttttacg agaggatgcg tatgaactcg ttttcacttc tgacaacccc
     2821 gtggttgcct gttcgtttca aagatggaac gacaggcaaa ctggcgcctg tcgatctggc
     2881 ggatgaaaat gttgtcgata ttgctgcgcc acgggcagat ctccagggag cggcatggca
     2941 gtttttgctg gggctactac aaaccagctt tgcgccaaaa gatcatcgtc gttgggatga
     3001 tatctgggaa gacggactgg aagttgaaaa gctacgggaa gcattgctgt ccttagaaca
     3061 cgctttccag tttgggccag attccccttc atttatgcag gattttgagg cgctcacggg
     3121 agataaagtt caggtcgctt cactactgcc tgagattccc ggcgctcaaa caacgaagtt
     3181 taataaagac cactttatca agcgtggtgt aacggaatac ctgtgcccac attgttctgc
     3241 gttagctctg ttctccctac agttaaatgc gccgtcaggt ggtaaaggct accgcaccgg
     3301 cttacgcggc ggtgggccga tgaccacgct gattgaatta caggaatatc agggcaatca
     3361 acaaactccc ttgtggcgca aaatgtggct caacgtgatg ccgcaggatg aagccgattt
     3421 accgctaccc aaaaaatttg acgatctggt tttcccctgg cttggcccga cgcgcaccag
     3481 cgaactggcc ggtgcggtgg taaccgatga tcaggttaat aaactccagg cgtactgggg
     3541 aatgccgcgg cgtattcgta ttgattttaa taccacgaca gtcggcaact gcgatatttg
     3601 cggcgagcag aacgacgcgc tcctgagcct gatgacgacc aaaaattacg gtgcgaatta
     3661 tgccatgtgg cagcatccct taacgcctta ccgtgtacca cttaaagagg gtggcgagtt
     3721 ttactccgtt aaaccacaac cgggcggttt aatctggcgt gactggttag gccttatcga
     3781 aacgggtaaa tcagaaaaca atacggaact tcccgcgctg gtggtgaaac tctttaatgc
     3841 cagcagtctg aaacaggcaa aagtgggcct gtggggattt ggttatgatt tcgacaacat
     3901 gaaagcgcgc tgttggtacg aacaccattt ccctctattg ctcaataaaa aagaaggcca
     3961 gataccaaag ctgcggctgg ctgcgcaaac ggcttcacgg attctaagtc tgttacggag
     4021 tgcattgaaa gaagcatggt tctctgatcc aaaaggtgca aggggtgatt ttagttttgt
     4081 ggatatcgac ttctggaaca aaactcagca tcgctttctg aggttcgtgc gccaaattga
     4141 agaaggtcag gatgcggatg aattactcag caaatggaat aaggaaattt ggttattcgc
     4201 gcgtcaggat tttgacgagc gtgtattcac taatccttat gagcccgtta atttggaacg
     4261 catcatgacc gcgcgcaaga aatattttac aacatcggcg gagaagcaaa gtgctaaagc
     4321 cgccagggag aaaaagcagg aggctgctga atgagtattg taaaagagga gcacaaagcc
     4381 acgctgcgca aatggcacga ggaattgcag gagaaacgcg gcgaacgtgc cagtctgcgg
     4441 cgtagtacga cggttaatga tgtttgtctg acggatggat ttcgcctgtt tctgaagaac
     4501 agacaaataa agtggcagga tgaacctgag tggcgtatca ccgcactagc gctgatagcc
     4561 gcagttagcg cgaatgttaa agccattgat gaacggcagc cttttgccgc gcagctggcg
     4621 gcggtaatgt caaaggggcg ttttactcgc ctgtcggcag taaaaacacc ggatgaatta
     4681 ctgcgccagt tacggcgcgc agtcagatta ttaaacggtt ccgtcaatct tgactctctg
     4741 gcagaggggg tcttccgctg gtgccaggag agcgacgacc tgctcaatca tcaccgccgc
     4801 cagcagcggc caaccgaatt tatccgtatc cgctgggcgc tggaatatta ccaggctggc
     4861 gacgccgata acgaacaaaa tcaataaagg actatctcat gacaacattt attcagcttc
     4921 atttgttaac cgcttaccct gctgccaacc ttaaccgtga tgatactggt gcgccaaaaa
     4981 ctgtggtact gggtggcgca acgcgtctgc gcgtttcctc acaaagtctg aaacgtgcgt
     5041 ggcgcacttc tgcacttttt gaacaggcac tggcgggcca tattggtatt cgtagtgggc
     5101 gtattgcgcg tgaggcggca actatcctga ttgagaaagg aatcgaagag aaaaaagcca
     5161 tcgaatgggc ggcaaaaatt gcggattatc ttgggaaagc taaaaacgac aaaaaaccaa
     5221 aagatccgct cactaacgcc gaaactgaac aattagttca tatcagcccg gcagaatttg
     5281 acgccgtaaa agcgctggcc catcagcttg cagaagaaaa gcgcgcgcca aaagaggaag
     5341 atctcgcgtt gttacgcaaa gaccgtatgg cggtagatat tgccatgttt ggtcgtatgc
     5401 tggcgaataa acccgagttt aatgttgaag ccgcctgcca ggtagcgcac gcatttggtg
     5461 tcagtgaaac gattgtcgaa gatgattttt tcaccgccgt tgatgatttg cgccaggctt
     5521 ctgaagatgc cggtgccggg catcttggtg aaaccggatt cggttctgca ctgttctaca
     5581 cctatatctg cattgataaa gatctgctgg tcgaaaacct cggcggtgac gaagcgttag
     5641 ctaatcagac catacgcgcc tttacggaag ccgcgcttaa agtctcccca accggcaaac
     5701 agaacagctt tgccagccgt gcctacgcct cctgggcgat ggcagaaaaa ggtaccgaac
     5761 aaccgcgttc tctggcggcg gctttctatg aacccattaa tggcacccgg cagttagagg
     5821 tggcggtgca gcgtattaca acgcttcgcg aaaacatgaa tacggtctac gaacagaaga
     5881 ccgaatgcgc aagttttgac gtgatgaaca aacagggaag catgaaggac gtgctggact
     5941 ttatctgcgc gtaaggggcg gcttatgagc caatatctga tttttcagct tcatgggcca
     6001 atggcatcct ggggcgtcga tgcccccggt gaagtgcgtc atacccatga actgccttcg
     6061 cgctcagcat tgctggggct gctggctgcc ggggtaggga ttcggcgtga tgataccgaa
     6121 cgcttaaacg cgtttaatcg ccactattca ctggtggttt gcgccagccg taacccgcgc
     6181 tgggcacggg attatcacac tatccagatg ccaaaagagg tgcgtaaagc gcgttatttc
     6241 agccgtcgcg aagagttgag cgaccctgat cttctgagcg cgattatctc ccggcgcgac
     6301 tactacaccg atgcctggtg gatggtggcg gtggcaacaa cagccgatgc gccttacagc
     6361 ctcgaacagt tgcaggacgg tttacgccat ccagtttttc cgctttatct ggggcgaaaa
     6421 agtcatccgc tggcattacc tcttgcgccg ttactgctcg aaggcaacgc gtgtgatgcc
     6481 ttatgtaatg cataccaaca gtatcaggat catttccata aactgaaagt ctcactcccg
     6541 aaacttcagg atgaatgctg gtgggaaggg gaacacgacg gcctggtcgc gagcaaaata
     6601 ttacgccgtc gggatgttcc tttaaatcgt cagcagtggt tgtttgggga acgcaccatc
     6661 aatcaggggc cgtggctcag caaggaggaa ccatgtacct ctcaagaata accttacata
     6721 ccggccagct ttcgcctgcg cagttgctgc atctggtgga tcgcggtgaa tatgtgatgc
     6781 atcagtggct gtgggatctt ttccctggcg gcaaagaaag gcaatttctt tatcgtcggg
     6841 aagaactcca gggcgcgttt cgcttttttg tcctctcgca ggaacgcccg gcggaaagtg
     6901 atacgttcac catagaatgc cggtcatttg cgcccgagct gcgtaccgga caacaacttt
     6961 gtttcaacct gcgggcgaat ccaacaatct gtaaatcggg caagcgccac gatctgctga
     7021 tggaggcgaa acggcaggtg aggggacagg cggaaggaag tgatgtctgg ttgcatcaac
     7081 aacaggcggc gctggactgg ctggcagcgc agggggaaag aagtggtttt acactactgg
     7141 atacctcggt tgatgcctac cgacaacagc aactccggcg ggaaaactcc cggcaactga
     7201 tccagttcag tagtgtggat tatacgggga tgcttacggt caccgatccc gggttatttc
     7261 tgcaacgtct tagccaggga tatggcaaaa gccgggcgtt cggctgtggt ctgatgctga
     7321 tcaaacccgg agcagaggcg tgacgtttgt accactgagt ccgatcccgt taaaagatcg
     7381 cacctccatg atcttcctcc agtacggtca gatcgacgta ctggacggcg ctttcgtgct
     7441 gatcgacaaa accgggatcc gcacgcacat tccggtggga tcggtcgcct gcattatgct
     7501 cgaaccggga acgagagttt cccacgcggc ggtgcatcta gccgccacgg tggggacgct
     7561 actggtctgg gtcggtgaag cgggcgttcg cgtttactcc tccggacaac ccggaggggc
     7621 gcgggcagat aaattactct accaggcaaa gctggcttta acggaagatc tacggttgaa
     7681 ggtggtgcgc aaaatgtatg aattacgttt tcgtgaacca ccgccagctc gccgttcagt
     7741 ggagcagtta cgtggaattg agggatctcg cgttcgccag acgtatgcat tactggcgaa
     7801 acaatatggt gtgaaatgga atggtcgcaa atacgatcct aaagactggg aaaaaggcga
     7861 tgttgtgaat cgctgcatca gtgctgctac atcatgtctg tacggtatat ctgaagcggc
     7921 agtattagcc gcgggatatg cgcccgctat tgggtttatc catagtggca aaccgctttc
     7981 atttgtttat gacatagccg atatcattaa atttgattcg gttgtgccaa aggcatttga
     8041 aatagcagcg aggcaacccg cagaacctga taaagaagtc agattagcct gtcgcgatat
     8101 tttccgtagc actaagttaa cgggcaaatt aataccgtta attgaggaag tccttgctgc
     8161 aggtgaaatt gaaccaccac aacccgcgcc ggatatgtta ccgcctgcca tccctgaacc
     8221 tgaaacgctg ggtgatagtg gtcaccgggg gcgcggcgga tgagcatggt cgtggttgtt
     8281 acagaaaatg tccctccgcg cttacgtgga cggctcgcaa tctggctact ggaagtgcgt
     8341 gccggtgtgt atgttggtga tacatcaaaa cgtattcggg agatgatctg gcagcaaatt
     8401 acccaactgg ctggttgcgg aaatgtggtg atggcctggg cgaccaatac cgagtcgggt
     8461 tttgaatttc agacctgggg agaaaacaga cgtattccgg tagatttgga tgggttacgt
     8521 ttggtttctt ttcttcctgt tgataatcaa taagttagac gttctttaaa aataaggaaa
     8581 tgttttaatt tagttggtag attgttgatg cggaataaat ttgtttaaaa acagttatgt
     8641 atgcttagtg tgttccccgc gccagcgggg ataaaccgtt gcccgcgcaa ttccgggagc
     8701 atccgcaatt gtgttccccg cgccagcggg gataaaccga cggacaaaat atatattgat
     8761 ttgcgaatta tgtgttcccc gcgccagcgg ggataaaccg gtaaagaaac tgccgacaaa
     8821 tccctgttcg ttgtgttccc cgcgccagcg gggataaacc gcccgtcacc gacgcgcagt
     8881 ggcgctaccg tgagtgttcc ccgcgccagc ggggataaac cgctaacgcc acattggcgg
     8941 aattggctgc tgatgtgttc cccgcgccag cggggataaa ccatgcggat taccggcaaa
     9001 acatgggagc aaaccgtgtt ccccgcgcca gcggggataa accgccgaac ggctggcgaa
     9061 gcaggtggct ggcgtagtgt tccccgcgcc agcggggata aaccggttta ccgccccgca
     9121 gaggcgctgg cagatccgtg ttccccgcgc cagcggggat aaaccgggat gacctgtcgc
     9181 taaaactcgc cgcgtacagt gttccccgcg ccagcgggga taaaccgtcc gtttggtcca
     9241 ccaaatgttt gatgcttcag tgttccccgc gccagcgggg ataaaccgtt cagggtgtga
     9301 ttccgcatac caagcagtaa gagttccccg cgccagcggg gataaaccgg gcgcactgga
     9361 tgcgatgatg gatatcactt agaattcccc gcccctgcgg tagaacaccc agctcccatt
     9421 ttcaaaccca tcaagacgcc ttcgccaact ccttcaccag aggtagcatt atccgcataa
     9481 cgtcacggca gcgacgttct attcttccag gaagtgcctt atcaatatgc tgttgattat
     9541 ccagtcttac gtcatgccag ctatttcccg cagggaatgc ggctgttttt gcgcgttgct
     9601 gataaccatc cttattccca agattccagt tagtcgcttc caccgaaagt acagcaatgc
     9661 ccgctttgtc gaatatttct gcgtcattac aacacccagt gcctttcgga taatttttat
     9721 tcaaacccgg attggtcgtt gcggctattc cgtgactgcg tgcaattgcc agcgccctgt
     9781 cgcgcgttaa tttccttact gcttcagggg tttttacacc gctgttgaaa tacaatttat
     9841 cgccaacaat taagttatcg agattaatca ccagcagcgt atttttcttt tcggtgtcac
     9901 tcatccgctt gagtaaattc tcagcgccta atttcccttc ctcttcgccg ctggtcgcca
     9961 caaatcgaat accatactcg gtaggcgtat ttttcaggcg ttctgccaat tccagcatga
    10021 cacctaaacc tgcggcgtta tcatccattc cttgtaacgt cagcccgccg agattggcat
    10081 cggcgtcagc atcgctcagc ggggcgtaag tatccagatg cgccataatg atgatctgct
    10141 gcggcgcttt gccttcatga gcggcaatca ccgtacttcc cgtcacgtta tgccagctct
    10201 tacgattatc gcgggcggta taaatatacc gactattaaa tgtccgaata tcactgcgat
    10261 aacccatttg ctgaaactgt tggcgaatat aatcggcaga taacatttct gcaggagttc
    10321 cggtcatgcg tcccggaaag aaagtagcaa tatgtcgtgc ctgagtatta gcaaaatcgc
    10381 caggtttagg tgacgaggcg tgtacgggga gaataaagca tacgccgagc gccagggcag
    10441 cggtacggtg gcgcaatgcg gaaaacatag tgagtcctta aataccatgc aaattttttt
    10501 accgccatag tatgaaactg ccgctgcgct aaaacaattt caaatcttcc taaacgcccg
    10561 aaatccggtg ccttaagcac tttttgatat tagctttgcc aaatcgttat tccgttaagg
    10621 aactactcat tctaattggt aatttcattc gttctcttac gctccctata gtcgaaacat
    10681 ctgatggcaa gaaaatagcg gtattgcaaa ggaacggtta tggatcaaat acgacttact
    10741 cacctgcggc aactggaggc ggaaagcatc cacattattc gcgaggtggc ggcagaattc
    10801 tcaaatccgg tgatgctcta ctctatcggt aaagattcca gcgtcatgct gcatctggcg
    10861 cgcaaggcgt tttatccagg tacgctgcct ttcccgttgc tgcatgtcga taccggctgg
    10921 aaattccgcg agatgtatga gttccgcgat cgtacggcga aagcctacgg ctgcgagctg
    10981 ctggtgcata aaaacccgga aggcgtggcg atggggatta atccattcgt gcacggcagc
    11041 gcgaaacata ccgatattat gaaaactgaa ggcctgaaac aggcgctgaa caaatacggt
    11101 tttgatgccg ccttcggtgg tgcgcgccgt gacgaagaga aatcccgcgc taaagagcga
    11161 atctactctt tccgtgaccg cttccatcgc tgggatccga aaaatcagcg cccagagctg
    11221 tggcacaact acaacgggca aattaacaaa ggcgaaagca tccgcgtctt cccgctctct
    11281 aactggaccg agcaggatat ctggcaatac atctggctgg aaaatatcga cattgtgccg
    11341 ctgtatctcg ctgcggaacg tccggttctg gaacgcgacg gtatgttgat gatgattgat
    11401 gacaaccgta tcgacctgca accgggcgaa gtgattaaaa aacggatggt gcgtttccgt
    11461 acgctgggct gctggccgct gaccggtgcg gtggagtcaa atgcacaaac actaccggag
    11521 atcatcgaag agatgctggt ttctaccacc agtgaacgtc agggccgcgt gattgaccgc
    11581 gaccaggcgg ggtctatgga gctgaaaaaa cgtcaggggt atttttaaga tgaacaccgc
    11641 acttgcacaa caaatcgcca atgaaggcgg cgtcgaagcc tggatgattg cgcaacaaca
    11701 taaaagcctg ctgcgttttc tgacctgtgg tagcgtcgat gacggcaaaa gtaccctgat
    11761 tggtcgcctg ctgcacgata cccgccaaat ctacgaagat cagctctcat cgctgcataa
    11821 cgacagtaaa cgtcacggca cccagggcga aaagctggat ctggcactgc tggtggacgg
    11881 cctgcaagct gagcgcgaac agggcatcac cattgacgtg gcttaccgct atttctctac
    11941 cgagaagcgt aaatttatta tcgccgacac cccagggcac gagcagtaca cccgcaatat
    12001 ggcgactggc gcatcgacat gtgaactggc gatcttactg atcgatgccc gtaaaggcgt
    12061 gctcgatcaa acccgtcgtc acagttttat ctccacactg ttggggatca aacatctggt
    12121 cgtggcgatc aacaaaatgg atctggtgga ttacagtgaa aagacgttca cccgtattcg
    12181 tgaagattat ctgacctttg ccgggcagct gccgggtaat ctggatatcc gctttgtgcc
    12241 gctctctgca ctggaaggcg acaacgtggc ttcgcaaagt gaaagtatgg cgtggtacag
    12301 cggtccaaca ctgctcgaag tgctggaaac cgtggagatc cagcgagtgg tggatgctca
    12361 gccaatgcgc ttcccggtgc agtacgttaa tcgcccgaat ctcgattttc gtggttacgc
    12421 cggaacgctg gcatccggtc gcgtagaagt cgggcaacga gtcaaagtgc tgccctctgg
    12481 tgtggaatca aacgtcgccc ggatcgtgac ttttgatggc gatcgcgaag aagcctttgc
    12541 cggagaagcg atcaccctgg tgctgacgga tgagatcgac atcagccgtg gcgatctgct
    12601 gctggcggca gacgaagcgt taccagctgt gcagagcgcg tcggtggatg tggtatggat
    12661 ggcggaacag ccgctttccc cgggccagag ttatgacatc aaaattgccg gtaagaagac
    12721 gcgtgctcgt gttgatggca ttcgttatca ggttgatatt aataacctta cccaacgcga
    12781 agttgaaaac ctgccgctga acggcatcgg cctggtggat ctcacttttg acgagccact
    12841 ggtgttagat cgttatcagc aaaatccggt taccggtggg ctgattttta tcaatcgcct
    12901 tagcaatgtg accgtgggcg ccggtatggt gcacgagcca gttagccagg caactgctgc
    12961 gccatctgaa tttagtgcat tcgaattgga attgaatgcc ctggttcgcc gccactttcc
    13021 acactggggc gcgcgcgatt tgctggggga taaataatgg cgctgcatga cgaaaacgtc
    13081 gtctggcata gccatccggt cactgtgcaa caacgcgagc tacaccacgg tcatcgtggt
    13141 gtagtgctgt ggtttaccgg cctctccggg tccggtaaat caacggtcgc cggggcgctg
    13201 gaggaggcgt tacataaact cggcgtcagt acgtatctgc tggatggcga taatgttcgc
    13261 cacggattat gcagcgatct cggttttagc gatgccgatc gtaaagagaa tatccgtcgc
    13321 gtcggtgaag tggcgaattt gatggttgaa gccggactgg tggtgctgac cgcatttatc
    13381 tcgccacacc gcgccgaacg ccagatggtt cgcgaacgcg taggagaagg gcgctttatt
    13441 gaagtgtttg tcgatacgcc gctggcgatt tgcgaagccc gcgatccaaa aggcttatat
    13501 aagaaagcgc gtgccggtga actgcgcaac tttacgggaa tagattccgt ttacgaagcg
    13561 cctgaatcgg cagaaattca tctcaatggt gaacaattag taacaaattt ggtacagcaa
    13621 ttattagatc tgttgagaca gaacgatatt atcagatcct gagacaccac cgggtttcca
    13681 tgcccggttc gtcaaagtaa caggatcaaa tatgcgtaat agccataaca ttacactaac
    13741 aaataacgac agccttactg aggatgaaga aaccacatgg tcactgcctg gtgccgtggt
    13801 cggttttatc tcctggttat ttgcgctggc gatgccgatg ttgatttatg gctctaacac
    13861 gctgttcttc tttatctaca cctggccttt ctttctggcg ctgatgcccg tcgcggtagt
    13921 ggtggggatt gcgctgcatt cattgatgga cggaaagcta cgctacagta ttgttttcac
    13981 cctggtgaca gtaggcatta tgtttggcgc actgtttatg tggctactgg gctaatttgt
    14041 actttcccgt ctcctctgtt cataattcaa accgtaacta ataatgagat tatgttctgc
    14101 acgccctggg tatacgtaac aatggacaaa tgtggtacat ttgcccgcgt tgtcgcggta
    14161 tccccaacag aggatgtaga gtcgtcttcg gatgcatggg atgatgatgc cgtttttcag
    14221 ggggcaggat gggtaaacta acgctgctgt tgctggctat tctggtctgg ctacagtatt
    14281 cgctgtggtt cggtaagaac ggtatacatg actataccct cgtcaatgat gatgtggcgg
    14341 cacagcaagc tacaaacgcg aaacttaaag cgcgaaacga tcaacttttt gccgaaattg
    14401 acgatctcaa tggcggccag gaggcgctcg aagagcgtgc gcgtaatgaa ctcagcatga
    14461 ccaggccggg cgaaactttt tatcgtctgg tgcctgacgc gtcgaagcgc gcacagtctg
    14521 cggggcaaaa caatcgataa atcagcccgg gaattaacat ggcaaccact catttggatg
    14581 tttgcgccgt ggttccggcg gccggatttg gccgtcgaat gcaaacggaa tgtcctaagc
    14641 aatatctctc aatcggtaat caaaccattc ttgaacactc ggtgcatgcg ctgctggcgc
    14701 atccccgggt gaaacgagtc gtcattgcca taagtcctag cgatagccgt tttgcacaac
    14761 ttcctctggc gaatcatccg caaatcaccg ttgtagatgg cggtgatgag cgtgccgatt
    14821 ccgtgctggc aggtctgaaa gccgctggcg acgcgcagtg ggtattggtg catgacgccg
    14881 ctcgtccttg tttgcatcag gatgacctcg tgcgattgtt ggcgttgagc gaaaccagcc
    14941 gcacgggggg gatcctcgcc gcaccagtgc gcgatactat gaaacgtgcc gaaccgggca
    15001 aaaatgccat tgctcatacc gttgatcgca acggcttatg gcacgcgctg acgccgcaat
    15061 ttttccctcg tgagctgtta catgactgtc tgacgcgcgc tctaaatgaa ggcgcgacta
    15121 ttaccgacga agcctcggcg ctggaatatt gcggattcca tcctcagttg gtcgaaggcc
    15181 gtgcggataa cattaaagtc acgcgcccgg aagatttggc actggccgag ttttacctca
    15241 cccgaaccat ccatcaggag aatacataat gcgaattgga cacggttttg acgtacatgc
    15301 ctttggcggt gaaggcccaa ttatcattgg tggcgtacgc attccttacg aaaaaggatt
    15361 gctggcgcat tctgatggcg acgtggcgct ccatgcgttg accgatgcat tgcttggcgc
    15421 ggcggcgctg ggggatatcg gcaagctgtt cccggatacc gatccggcat ttaaaggtgc
    15481 cgatagccgc gagctgctac gcgaagcctg gcgtcgtatt caggcgaagg gttataccct
    15541 tggcaacgtc gatgtcacta tcatcgctca ggcaccgaag atgttgccgc acattccaca
    15601 aatgcgcgtg tttattgccg aagatctcgg ctgccatatg gatgatgtta acgtgaaagc
    15661 cactactacg gaaaaactgg gatttaccgg acgtggggaa gggattgcct gtgaagcggt
    15721 ggcgctactc attaaggcaa caaaatgatt gagtttgata atctcactta cctccacggt
    15781 aaaccgcaag gcaccgggct gctgaaagcc aatccggaag actttgtggt ggtggaagat
    15841 ttgggctttg agcctgatgg tgaaggtgag catattctgg ttagaatcct caaaaacggc
    15901 tgcaataccc gttttgtggc ggatgcactg gcgaaattcc tgaaaattca tgcccgtgaa
    15961 gtcagcttcg ctgggcaaaa agacaaacat gctgttacgg aacagtggtt atgcgctcgc
    16021 gtgccgggca aggaaatgcc cgatctgagc gcctttcaac tggaaggctg ccaggtgctg
    16081 gagtatgcgc ggcacaagcg caagctgcgt ttaggggcgc tgaaaggaaa cgcctttacc
    16141 ctggttctgc gcgaagtgag caatcgcgat gacgttgaac aacgtctgat cgatatttgc
    16201 gtaaaaggtg taccgaacta cttcggtgcc caacgttttg ggattggcgg tagcaacttg
    16261 cagggggcgc agcgctgggc gcaaaccaat actccggtgc gcgatcgcaa taaacggagt
    16321 ttttggttgt cggcagcccg cagtgcgttg tttaatcaga ttgttgctga gcgcctcaaa
    16381 aaagcagacg ttaatcaagt tgttgacggc gatgcgctac aattagccgg acgtggtagc
    16441 tggtttgtag caaccaccga agaactggcg gaattacagc gtcgcgtcaa cgataaagag
    16501 ttgatgataa ccgccgcatt gccaggcagt ggcgaatggg gaactcagcg tgaagcgctg
    16561 gcattcgaac aagcagctgt cgccgcagaa actgaattac aagctttact ggtgcgcgaa
    16621 aaagttgaag ccgcgcgcag agcgatgctg ctgtatccgc aacaattaag ctggaattgg
    16681 tgggatgacg tcaccgtaga gatccgtttc tggcttccgg cgggtagttt tgcaaccagc
    16741 gttgtcaggg aacttatcaa cacaacaggt gattatgcgc atattgctga gtaatgatga
    16801 cggggtacat gcacccggta tacaaacgct ggcgaaagcc ttgcgtgagt ttgctgacgt
    16861 tcaggtggtc gcccccgatc gtaaccgcag cggcgcttca aattctctga cactggaatc
    16921 ctccctgcgc acgtttacct ttgaaaatgg tgatattgct gtgcaaatgg gaaccccgac
    16981 cgattgcgtc tatcttggcg tgaatgctct gatgcgtccg cgcccggaca ttgttgtgtc
    17041 cggaattaac gccgggccga atctggggga tgatgttatt tattccggta cggtagccgc
    17101 cgcgatggaa ggccgtcatt taggttttcc ggcgcttgcc gtctcgcttg acgggcataa
    17161 acattacgac actgccgcgg cggtaacctg ttcaattttg cgcgcactgt gtaaagagcc
    17221 gctgcgcacc gggcgtattc ttaatattaa cgttccggat ttacccttgg atcaaatcaa
    17281 aggtattcgc gtgacgcgct gcggtacacg acatccggca gatcaggtga tcccgcagca
    17341 agatccgcgc ggcaatacgc tgtactggat tggcccgccg ggcggtaaat gtgatgctgg
    17401 tccggggacc gattttgctg cggtagatga gggctatgtc tccatcacgc cgctgcatgt
    17461 ggatttaact gcgcatagcg cgcaagatgt ggtttcagac tggttaaaca gcgtgggagt
    17521 tggcacgcaa tggtaagcag acgcgtacaa gcacttctgg atcaattacg tgcgcaaggt
    17581 attcaggatg agcaggtgct gaatgcactt gccgccgtgc cgcgtgaaaa attcgttgat
    17641 gaagcgtttg aacaaaaagc ctgggacaat atcgctttgc cgataggtca ggggcagaca
    17701 atttcgcagc catatatggt ggcgcgaatg accgaattac tcgagctgac gccgcagtcg
    17761 cgggtgctgg aaattggcac cggttcggga tatcaaacgg caatcctggc gcatcttgtc
    17821 cagcatgttt gctcggttga acggattaaa ggcttgcagt ggcaggcacg tcgccgcctg
    17881 aaaaatcttg atttacataa tgtttcaacc cgtcatggcg atggatggca aggttggcag
    17941 gcacgtgcgc cgtttgacgc tatcattgtt acggcggcac cgccggaaat tccaactgcg
    18001 ctaatgacgc agctggacga aggcgggatt ctcgtcttac ccgtagggga ggagcaccag
    18061 tatttgaaac gggtgcgtcg tcggggaggc gaatttatta tcgataccgt ggaggccgtg
    18121 cgctttgtcc ctttagtgaa gggtgagctg gcttaaaacg tgaggaaata cctggatttt
    18181 tcctggttat tttgccgcag gtcagcgtat cgtgaacatc ttttccagtg ttcagtaggg
    18241 tgccttgcac ggtaattatg tcactggtta ttaaccaatt tttcctgggg gataaatgag
    18301 cgcgggaagc ccaaaattca ccgttcgccg cattgcggct ttgtcactgg tttcgctatg
    18361 gctggcaggc tgttctgaca cttcaaatcc accggcaccg gtcagctccg ttaatggcaa
    18421 tgcgcctgca aatactaatt ctggtatgtt gattacgccg ccgccgaaaa tggggacgac
    18481 gtctacagcg cagcaaccgc aaattcagcc ggtgcagcag ccacaaattc aggctactca
    18541 acaaccgcaa atccagccag tgcagccagt agctcagcag ccggtacaga tggaaaacgg
    18601 acgcatcgtc tataaccgtc agtatgggaa cattccgaaa ggcagttata gcggcagtac
    18661 ctataccgtg aaaaaaggcg acacactttt ctatatcgcc tggattactg gcaacgattt
    18721 ccgtgacctt gctcagcgca acaatattca ggcaccatac gcgctgaacg ttggtcagac
    18781 cttgcaggtg ggtaatgctt ccggtacgcc aatcactggc ggaaatgcca ttacccaggc
    18841 cgacgcagca gagcaaggag ttgtgatcaa gcctgcacaa aattccaccg ttgctgttgc
    18901 gtcgcaaccg acaattacgt attctgagtc ttcgggtgaa cagagtgcta acaaaatgtt
    18961 gccgaacaac aagccaactg cgaccacggt cacagcgcct gtaacggtac caacagcaag
    19021 cacaaccgag ccgactgtca gcagtacatc aaccagtacg cctatctcca cctggcgctg
    19081 gccgactgag ggcaaagtga tcgaaacctt tggcgcttct gaggggggca acaaggggat
    19141 tgatatcgca ggcagcaaag gacaggcaat tatcgcgacc gcagatggcc gcgttgttta
    19201 tgctggtaac gcgctgcgcg gctacggtaa tctgattatc atcaaacata atgatgatta
    19261 cctgagtgcc tacgcccata acgacacaat gctggtccgg gaacaacaag aagttaaggc
    19321 ggggcaaaaa atagcgacca tgggtagcac cggaaccagt tcaacacgct tgcattttga
    19381 aattcgttac aaggggaaat ccgtaaaccc gctgcgttat ttgccgcagc gataaatcga
    19441 cggaaccagg cttttgcttg aatgttccgt caagggatca cgggtaggag ccaccttatg
    19501 agtcagaata cgctgaaagt tcatgattta aatgaagatg cggaatttga tgagaacgga
    19561 gttgaggttt ttgacgaaaa ggccttagta gaagaggaac ccagtgataa cgatttggcc
    19621 gaagaggaac tgttatcgca gggagccaca cagcgtgtgt tggacgcgac tcagctttac
    19681 cttggtgaga ttggttattc accactgtta acggccgaag aagaagttta ttttgcgcgt
    19741 cgcgcactgc gtggagatgt cgcctctcgc cgccggatga tcgagagtaa cttgcgtctg
    19801 gtggtaaaaa ttgcccgccg ttatggcaat cgtggtctgg cgttgctgga ccttatcgaa
    19861 gagggcaacc tggggctgat ccgcgcggta gagaagtttg acccggaacg tggtttccgc
    19921 ttctcaacat acgcaacctg gtggattcgc cagacgattg aacgggcgat tatgaaccaa
    19981 acccgtacta ttcgtttgcc gattcacatc gtaaaggagc tgaacgttta cctgcgaacc
    20041 gcacgtgagt tgtcccataa gctggaccat gaaccaagtg cggaagagat cgcagagcaa
    20101 ctggataagc cagttgatga cgtcagccgt atgcttcgtc ttaacgagcg cattacctcg
    20161 gtagacaccc cgctgggtgg tgattccgaa aaagcgttgc tggacatcct ggccgatgaa
    20221 aaagagaacg gtccggaaga taccacgcaa gatgacgata tgaagcagag catcgtcaaa
    20281 tggctgttcg agctgaacgc caaacagcgt gaagtgctgg cacgtcgatt cggtttgttg
    20341 gggtacgaag cggcaacact ggaagatgta ggtcgtgaaa ttggcctcac ccgtgaacgt
    20401 gttcgccaga ttcaggttga aggcctgcgc cgtttgcgtg aaatcctgca aacgcagggg
    20461 ctgaatatcg aagcgctgtt ccgcgagtaa gtaagcatct gtcagaaagg ccagtctcaa
    20521 gcgaggctgg ccttttctgt gcacaataaa aggtccgatg cccatcggac ctttttatta
    20581 aggtcaaatt accgcccata cgcaccaggt aattaagaat ccggtaaaac cgagaatggt
    20641 cgttaacact gtccaggttt tcagaccgtc tgctaccgac aaccccagat atttggtcac
    20701 aatccagaac cctgagtcat taatatgtga cgcgccaagc ccaccaaagc aggctgccag
    20761 cgtcaccaat acgcactgaa tcggattcaa tcccatcacc gcttctgaga gtaacccgcc
    20821 ggttgtcagg attgctacgg ttgctgaccc ctgcgatgca cgcagagcca gtgaaataat
    20881 aaatgcggct ggtaacagag gcaggtcaat catttgtagc atattggcaa gggctttgcc
    20941 gacgcccgat tccaccagca ctttgccaaa tacccctcca gcaccagtaa ccaaaatcac
    21001 taccgccgca gtgggaaggg ctgagcccat aatgtcgctg gtgtgttgta agctccagcc
    21061 gcgacgtaaa gccaataacc agaatgccag caccagcgca atcattagag ctaccattgg
    21121 tgagccgatc agctgtagcg taccaagcag gggatgcgaa ggcggcatca gtgttgcgga
    21181 aaccgtaccc gccatgataa tcgcgatagg aataacaatt agcgaggtga ccagcgcgac
    21241 gcccggtgga tttattttat cgcttaattt tgtcgcgcct tcctcactgg ccggagccag
    21301 ttgcatctgt tccagtactt ccaccgacat cgcatattgg tgcttattga ttattttcgc
    21361 tgcaaagtag ccaacaatcc ctacgggaat agaaatcgca ataccgatga tggttagcca
    21421 gccgatgtct gcgtggagta accccgctgc ggcgacaggg cctggatgcg gcggtaccgc
    21481 cacatgtaca gttagcatga tcccagcgac aggcaggcca aatttgagtg gcgatatttt
    21541 ggcaaccttg gcaaaaccgt aaatgattgg cgcaagaata ataaagccga catcaaagaa
    21601 gacgggaata ccgaggaaga acgctgccag agtcagcgca gcgatagttc gtttgtcacc
    21661 taacttgcga ctgaaataat tagccagtga ctctgcacca ccagagtgtt cgatcatacg
    21721 ccccagcata gcgcccagac caataataat agtgacggaa ccaagcacac cgcccatccc
    21781 ggcgatcatc actttaccca cttcgcccgc cggtataccc gccgcaagtg cgactaacag
    21841 gctgacgagg agcagagcaa cgaatggttg tacctttgcc ttgatgacca gcagcaacag
    21901 catgattacg ccagttaacg caatgcataa caatgtaatt gtggacatgg gaaaccctgt
    21961 ctgaaagtta tagttaacct accccatccg tagatggggg gatgtatggg tacgttgtaa
    22021 ttagggattt aacgaattag cgccaggcgt caaaccagcc aagcccttct tcggtgaggc
    22081 cacgaggttt atattcacaa ccgatccagc cctgatatcc cacctcatcg aacaggcgga
    22141 acagccacgg atagttgatt tctccatcgt ccggttcatg tcgatcaggt agtccggcaa
    22201 tttgtacgtg cgcatatttc ccggcgtagt cgcggattaa atgcgtcagg ttgccatcta
    22261 ctttttgcgc atgaaaagta tctagttgaa taaacacgtt atctcgcgca acctcttcaa
    22321 caatagccag tgcctgatac tggctggaga agagataatg aggcttaacg tcggggctga
    22381 gtgcttcaac taatattcgc ttgccgtgta gcgcaaagcg gtcggcagcg tagcggagat
    22441 tatcgataaa tactgcccgg taccgttcag cgtcttcgcc agcgggcacg acgcctgcca
    22501 tcacatggac ttgttcacaa ttgagcgcca atgcatattc cagtgccagg tcgatgtctg
    22561 cgtgtgcttc gtgctcacgt ccgggaaggg cggataatcc ccattccccc gcattaatat
    22621 ctccgggagc ggtattgaac agcgccagtg tcagatggtt ttgctccagt tgcttttgga
    22681 tttgcagggt ggagtagtca tagggaaaca gaaattccac agcatcgaac ccggcttttc
    22741 gcgctgcggc gaagcgttca ataaaaggca cttcggtgaa catcatggat aaattagctg
    22801 caaaacgagg cattgcatta actccttaat tccgcaattt cacctgcggt cagataacgg
    22861 atcgggcggt caccgagaat aaaaatcagc tttgccgttt cctccagctc ttccatattg
    22921 ttggcggctt cttgcaggct ttcaccgcaa accactgggc catgatttgc cagtaaaaaa
    22981 gcctgattgt ctgctgccag ttccgccaga tcctgtgcga tgcgtttatc gcccggtcgg
    23041 taataaggca ccagcgggac atttcccatc cgcatcacca cgtatggtgt gaacggacga
    23101 ataacgttgc tgctgtccag cccttgcagg caggaaagcg ccgtcgacca tgtgctgtgc
    23161 aaatgcacca ccgctttaca gcgcggattg ttgcgataca gcgccagatg aaagagcacc
    23221 tctttcgagg gtttgtcacc acttaaccat tcgccatccg cggcgacttt ggaaagccgc
    23281 tgcggatcga gattgcccag gcatgaacct gtcggtgtcg ccagtaaatt cccgtcaggt
    23341 aaaagcagcg acagattgcc agccgaaccg gttgcatagc cgcgctgaaa gaatgaactg
    23401 gcaatccgcg tcatctcctc tcgcaaagac tgctctactt ttgcgaaatc gctcatgata
    23461 aaaactctct ttgggctcgt gaaaaaaagg cttcatcacc gaagttgcca gatttaaggg
    23521 cgagtgagac aggcttatcc agtgcgttta cccacggcac gccgggggaa atggttgggc
    23581 caatatgaaa cccttttatg cccaggctct gtgtgactac gccggaggtc tcaccgcctg
    23641 cgacaataaa gcgtgtcacg ccttccgctg ctaaccgcgc cgctagttga gaaaacagag
    23701 tttctactgc ctgactggct ttttgtgcac cgtattgctg ttgaattgct gccaatgcgt
    23761 cagtgctggc ggtggcaaaa accagtggag caagtacact ttcctggccc agaacccact
    23821 ctgccagttc gtgtgcataa gcggccagag tttcggttga gaggcagcgt gccacatcaa
    23881 cttcacgggc tggtgcaatt tgacggtaat gtgccacctg gcggttggtc atttgagagc
    23941 atgaaccgga gagcactacg ccgcgcccag cgagcggatg ccctgcttcg cgagcctggt
    24001 taccgttttc ttgcgcccac tgccgggcca ggccaatcgc cagaccagaa ccgcccgtta
    24061 ccagtggggc atcgcgcaag gcttctccct gaatttccag atggtgttcg gtcagcgcat
    24121 caagcaccgc gtagcggtag ccctcttgct gtaagcgagc cagctcttga cgaacggcat
    24181 ccacaccttg ttcgaaaaca tgtgccgaaa cgacgccgca gcgccctgtg gattgcgctt
    24241 caaccagacg gggaagatag ctgtcggtca tgggatttac cgggtgatgg cgcatcccgg
    24301 attcggccag cagttgattc attacgaaca aatacccctg ataaaccgta cgtccgttga
    24361 ccggcagggc cggagagaag accgtaaacg gcgtgtcgag agcatccatt aatgcatcgg
    24421 taaccgggcc gatattacct ttcgccgtac tgtcgaaagt agagcagtat ttgaaataga
    24481 tctgtttgca accttgctgt tgcaaccagc tcagagccgc cagcgattgc tgtgtggctt
    24541 caaccaccgg acaggagcgc gttttcaggc tgatcaccag tgcgtcgatt gcttccggca
    24601 ttttacctgt tggaacaccg ttaatttgta ccgttggtag accgttttcc accagaaaac
    24661 tggcgatatc cgtcgcgccg gtaaaatcat cggcgataac gccaatcttg atcatgattt
    24721 cgctcccggt agagtgatgc cagagaaaat cttgataact gcgctatcgt cttctttccc
    24781 gtaacctgcg ttactggcgc tggtgaacat attcaatgct gttgaggcca atggcagcgg
    24841 gaagtgcagg gctttggctg tatcggcaac cagaccaaga tccttaacaa aaatatcgac
    24901 ggctgaatgc ggggtgtaat cgccatccac cacatgacgc atccggtttt cgaacatcca
    24961 ggaatttccg gcggcattgg tcacgacgtc atacatcaca tccagcggga tccccgcacg
    25021 ggctgcaagt gccatcgctt cggctccggc agcaatatgt acgcccgcta acaactggtg
    25081 aataattttt acggtcgaac ctagtcccgg ttctgcacct atgcgataaa cttttccggc
    25141 aacggcttcc agcacgggtg ccagtcgttc aaaggcaata tcgctaccgg aggccatgac
    25201 agtcatttca ccgttagcgg cttttactgc accaccagaa actggcgcat ccagcatttc
    25261 cagatcgaat ccagccagag cggtagcaat ttcttgcgca tcagcactag cgatagtgga
    25321 agaaaccatt actgccgtac cgggtttcag atgttgtgca acgcctgttt caccaaacag
    25381 cacctgttta acctgggccg cattgaccac cagcaccagc agagcgtcca gtttttcggc
    25441 aaacgtcgcg gcgttatcag aaaccccgca agcacctgcc tctttcaacg tagcgcaggc
    25501 attgctgttc aggtctgcgc cccaggtaga aagacctgcg cggacacatg acagtgctgc
    25561 tcccattccc attgacccta agccaacgat accgacatga aactcagatc ccgttttcat
    25621 ctgctcttct tgttaattta agtgatattt tgtttgatat tgtgaatata agcgctggaa
    25681 gataacgata tggtgagctg attcacataa attaacattg tgtgttattt tatgtgaact
    25741 aagcgttagt tgcgccgcgc acgtttcgca ggcaaatagc gtagaatgtc agcaggacaa
    25801 aggaaggagc aaaagttgat acccgtagag cgtcgccaaa tcatccttga gatggtagct
    25861 gaaaaaggca ttgtcagtat tgctgaacta acggacagaa tgaatgtgtc acatatgacc
    25921 attcgtcggg atttacaaaa actggagcag cagggagccg ttgtgctggt gtccggaggc
    25981 gtccagtctc cgggacgcgt ggcgcatgaa ccttctcatc aggtaaaaac tgcgctggca
    26041 atgacgcaaa aagcggctat tggcaagctg gcggcaagtc ttgttcagcc gggaagctgt
    26101 atctatctgg atgcgggaac gaccacgtta gcgatagcac agcatctgat tcacatggag
    26161 tcactgactg tggtcacaaa cgatttcgtt attgcgaact acttgctcga caacagtaat
    26221 tgcacaatta ttcacactgg cggtgcagtg tgtcgggaaa accgttcctg tgtcggggaa
    26281 gccgctgcga ccatgctgcg cagcctgatg attgatcagg cttttatttc tgcatcgtca
    26341 tggagtgtgc gggggatttc tacgccagcg gaagataaag tcacggtgaa acgggcgatt
    26401 gccagtgcca gccgccagcg agttttggtc tgtgatgcga cgaaatatgg tcaggtggcg
    26461 acatggctgg cgttaccatt aagcgagttt gatcagatta tcacagacga cggtttgccg
    26521 gagagtgcca gtcgcgcgct ggcgaagcag gatctctctt tgctggtagc gaaaaatgaa
    26581 taatggcctg caataacatt tggttactca tgcttcacag aagaagcatg agactacttt
    26641 attttataaa atgacagccg cccgcttttc ggcgtgccgg tatcaatata aatctggtta
    26701 gcgaacgtct gaatgttatc aaacatcata tgtccaaata taaaataatc agcgccgttt
    26761 atttgttgta actcgccatt aagcgatttc tgcacacgat caacaggcca gagtaattcg
    26821 ctctccgcta tttctttacc aaagagatat tcactccccg gataatctgc atgtgcgatg
    26881 acatatttta tgttgtcgtt agtgatttca ataatatgtg gaaggtgatg gaatttcagc
    26941 aacagatcta ttgcctcttg ttgctctgaa tcatttaaat cgaaaaacca gtcaccaccg
    27001 ctggcaagcc acatattgcc atcgccagtt tcgaatgcat ccaacgccat cgcttcgtgg
    27061 ttgcctttaa ccgacgtaaa ccagggttgg tttagcaggc gcagcacgtt aagactcttc
    27121 ggcccacgat caatattatc gcctgtggaa ataagtaagt cggtttcagg gtaaaaagag
    27181 agttgatgta aacgggattg taataactga tattcaccat gaatatcacc aacgacccat
    27241 atatggcgat agtgatgggc attgattttt tgatagcgtg tagatggcat ggttttaccc
    27301 tgtaaaataa gctttcctat tatacagggt atttttattt gattcgtcag ttgtcgttaa
    27361 tattcccgat agcaaaagac tatcgggaat tgttattaca ccagactctt caagcgataa
    27421 atccactcca gcgcctgacg cggagtcagt gaatccgggt cgagattttc cagtgcttcg
    27481 accgcaggcg aagtttcttc tggcactgac agcaaagaca tctgcgtacc atccacttgc
    27541 gtcgcggcgg cgttcggcga aatgctttcc agctcacgca gtttttgccg tgcgcgctta
    27601 atcacctctt ttggcacgcc cgccagagct gcaaccgcca ggccgtagct tttgctcgcc
    27661 gcgccatcct gcacgctatg cataaaggca atggtgtcgc cgtgctccag cgcatcgaga
    27721 tgcacgttgg cgacgccttc cattttctcc ggtaactggg tcagctcgaa atagtgggta
    27781 gcaaataacg tcaatgcctt aatcttattc gccagatttt ccgcgcacgc ccacgccagc
    27841 gacagaccat cgtaggtgga cgttccacgc ccgatctcat ccattaacac cagactgtat
    27901 tcggtggcgt tatgtaaaat attggcggtt tcagtcatct ccaccataaa ggttgagcgc
    27961 ccggaagcca ggtcatctgc cgcgcctacg cgggtaaaga tgcgatcgat aggtccaatc
    28021 tcgacttttt gtgccggtac atagctgccg atgtaggcca tcagcgcaat cagtgcggtc
    28081 tggcgcatat aggtactttt accgcccatg ttcgggccgg taataatcaa catacggcgc
    28141 tgcggcgaca gattcagcgg gttagcgata aatggctcat tcagtacttg ttcaactacc
    28201 ggatggcgac cttcggtaat gcgaatgccc ggtttatcaa tgaaggtcgg gcaggtgtag
    28261 ttcagggtat aggcccgttc cgccaggtta accagcacgt cgagttccgc cagcgcgctc
    28321 gcgctctgtt gcaacgcttc cagatgcggc aacagcaggt cgaacagctc ttcataaagc
    28381 tgtttttcca gtgccagtgc tttgcctttt gaggtgagaa ctttatcttc gtactctttt
    28441 agctctggaa tgatgtagcg ctcggcgttt ttcagcgtct ggcgacgcat gtagttgatg
    28501 ggtgccagat ggctttgccc acggctgatt tgaatgtagt agccgtgcac cgcattaaag
    28561 ccaactttca gcgtgtccag gccggtacgt tcacgctcgc ggacttccag acgctccaga
    28621 taatcggtcg cgccgtcagc cagcgcgcgc cactcatcca gctcttcgtt atagcccgat
    28681 gcgataacac caccgtcgcg taccagcacc ggcggtgtgt cgatgattgc tcgctccagc
    28741 agatcgcgca gctcggcaaa ctcgcccatc ttctcacgta gcgcctgtac cggtgcacta
    28801 tcgacagttt ctaactgcgc acgcagctcc ggcagttgct ggaaagcgtg gcgcatacgg
    28861 gccagatcgc gtgggcgagc agttcgtaaa gccagacgtg ccagaatacg ttccaggtcg
    28921 ccgacctgac gcagtaccgg ctgcaactcg gcggtgaaat cctgcaatgc gccaatagtt
    28981 tgctggcgct caagcaacac gcgggtatcg cgcactggca tatgcagcca gcgtttcagc
    29041 atacgactac ccatcggcgt gacggtgcag tcgagcacag aagccagcgt attttccgca
    29101 ccgcccgcca ggttctgagt aatttccagg ttacgacgtg tcgcggcatc cataatgatg
    29161 ctgtcctgct cacgttccat ggtgatagaa cgaatatgcg gcagggtcgt gcgttgggta
    29221 tctttcgcat actgcaacag acaaccggca gcacaaagtc cgcgcggcgc gttctcgacg
    29281 ccaaaaccga ccagatcgcg ggtgccaaat tgcagattca actgctggcg cgcggtgtcg
    29341 atttcaaact cccacagcgg gcgacggcgc aggccgcgac ggccttcaat cagcgacatc
    29401 tcggcgaaat cttctgcata cagcagttcc gccggattag tgcgttgcag ctctgccgcc
    29461 atcgtttcgc ggtcggccgg ttcgctcagg cgaaaacgcc cggagctgat atccagcgtc
    29521 gcgtagccga aacctttgct gtcctgccag atagccgcca gcaggttgtc ctgacgctcc
    29581 tgtaacaggg cttcatcgct gatggtgcct ggcgtaacga tacgcacaac tttgcgctca
    29641 accggacctt tgctggtcgc cggatcgcca atttgttcgc agatggcaac ggactctccc
    29701 tgattcacca gtttggcgag atagttttcc accgcatggt agggaatccc cgccatcggg
    29761 atcggctctc ccgccgaagc accgcgtttg gtcagtgaaa tatccagcag ttgcgacgcg
    29821 cgttttgcgt cgtcataaaa cagttcataa aaatcaccca tccggtaaaa cagcaggatc
    29881 tcgggatgct gggctttcag cctgagatac tgctgcatca tgggcgtatg ggcgtcgaaa
    29941 ttttctattg cactcatggg gttatgtccg gttccctgat attaaatggg gtgtgatggt
    30001 ttttattttc gttgtaatca cataaggcgc acatgatacc ggagttaatc atttcagggg
    30061 aagaagcgaa cggcgagttc ttgatgctgg ttccgggaaa gatgctgttt tgcagctttt
    30121 gcgtgatgct tatgaagcaa gtacgcaaaa ttgcgtcggt tgaataaacc tcctccttca
    30181 gagccatagt cccctttcaa attaagttgt aatataaata caccttaaga gtatggaggc
    30241 agcatgtccg gcaagcgtat ctctcgtgaa aaactgacga ttaaaaaaat gatcgatctt
    30301 tatcaagcga aatgcccgca ggcgtcagcg gagccggagc attacgaggc gttgtttgtt
    30361 tacgcgcaaa agcggctgga taaatgtgtg ttcggcgaag agaaaccagc ctgtaagcag
    30421 tgtccggtgc actgttacca gccagcaaag cgtgaagaga tgaaacagat tatgcgttgg
    30481 gccgggccgc gaatgctgtg gcgtcatccg atcttaaccg tgcgtcatct catcgatgac
    30541 aagcgtccgg tgccagaact gccggaaaaa tatcgtccga aaaaaactcg tgaatagtat
    30601 gccggacagg cagatctgtc cggcaatttg cagttaaatc aatgccgatt tatcaattcc
    30661 aagccgcttc atccgtgaca gcaaggtcgt gcgtttcagc cccagacgtt gcgcagcgcc
    30721 tttaggcccg gcaaccacgc cgttggtttc tttcagcacg cgaacaatca actgatattc
    30781 atcttcgccc tcctgggcga caaccgttgc ggcaggcggc gtttcaggtt ccggtaaaac
    30841 aatatctggc aatgacagct gcagcacgtt accgcgtgtt agcaataccg cgcgctcaat
    30901 gacgttttcc agttcgcgta cgttacccgg ccactccatg ttgctcaagg tgcgcagcgt
    30961 ctcggcagga atgctgtcga tattgcgccc cagacgacgg gcaattttga aggtaaaggc
    31021 tttcgccagc agcggaatat cttccggacg ctcgcgtagt ggcggcaggt gaatcgggaa
    31081 tacgttcagg cggtaataga gatcgctacg gaactcacgg tcggcgacca tttttttcag
    31141 atcgcggtta gtcgcggcga ttagacgcac gtccgtctga atgattttgt tgctgccgag
    31201 acgttcaaac tcctgttcct gcaatacacg cagcaacttc ggctgtaact ccagtggcat
    31261 atcgcccact tcgtcgagga acagggagct tttatccgcc agttcaaaac gaccaatgcg
    31321 ctgggcgctg gcaccggtaa aagccccacg ctcatgacca aacagatcgc tttccagcaa
    31381 tccggcaggc atcgccgcgc agttcatttt gaccatgcgg cgattattac gcccactgag
    31441 attatggatc gcacgggcaa tcagctcttt acccgtgcca gtttcaccga ggatcagcac
    31501 ggtactgtca ctttgcgcca ccatttcaac ttgtttaagc acgctgtaca tggcttcgct
    31561 gcggccaata atctcgccaa attcactatc aacattgttg agctgctcgg tcagggcgag
    31621 gttttcatca accagccgtt ctttcagacg atggatttcc tgataggcga gggcgttatc
    31681 gacagcgatt gccacacgtt cggcaatctg gcgcagtaaa ttcagattgg tagtggtaaa
    31741 cactttctct tcgcattgcg ccagtttcag cacgcccagc atggtgtcgc cagacatcag
    31801 cggtaacagg cacaaggttt gaatctggtt gccccaggtg tcgaacaaca tgcgttcata
    31861 gggggctaaa tcgtcccgct cgtggagatt gatcagcagc atctctttac ttttgaacac
    31921 gcgttcggtg agggttccgg cttcatcgac ttcgctctgt tcgtgggcgg gatgttgttt
    31981 atcaagatag tgagtggagt agatgttgag tttgttttta cggtggctgc gtaagacaat
    32041 gctgatatcg tcaatgtcga aatagtaatg gatttctttg gcgacttcgc tgaccagttc
    32101 gtccatatcc aggcgggaaa gcaccgcgtt ggtaatggca accaggatgc ggaagttatc
    32161 gcgctcccgg cataacaact catagtcgac attgttgacg acgcggctct ggatttgttc
    32221 ggtgacgaca gaaacgatct gcgtaaatgt ttgcagacga ttgaactctt tttcgctcca
    32281 ggggcgatcg tcataacgaa taaattcaca gccaccaaaa atatgccctt ccgccgccag
    32341 tggcatcagg caatagtgac caaatttggg gtatagccca cctgcggcca gctgcggcca
    32401 ggtttcacaa aattcttcgt aactgcaatg cagcgtatca gggcgcgaca aaatgctgcg
    32461 taccggaccg tgtgccagaa cagtttcgtc ttcatattta atgggggtgt ctttttcacg
    32521 cgatgcgtaa taagacgcac gttgagtctg cgcttgccac aacacaatag ccgcgttgtc
    32581 ggcgagcgca gaacgcttta ccagttgcga aagagcctca cacagcgagg ccagatcggg
    32641 ctgctgcaat agtgtccgag tgatgtcgaa caacccttgc tgtccgagat cactcatcgg
    32701 tgtatatgac attttgttct tccaggaaag caccgcaaag cggtgcaaaa ggtcattgac
    32761 taactatagg agatttagaa ttttattagc atatacgcgg aagcggttcg gcgtgtggta
    32821 aatcgagggt tcgtttcacg ccatacagac cggcaagacg aacaccttta cgttccacca
    32881 cttcaccaat cagcgccgcg tctttcccca gtggatggga atgtaacgct gccagcactt
    32941 gctctgccgc gttgcgttca acagctatta ctagtttgcc ttcgttggca aagttcaggg
    33001 cgtccagtcc cagcaattcg caaacgccac gcacggcagg tttaacaggc agtgccgctt
    33061 ctgaaagttc aataccacaa ccgcaggctg ccgcgaactc atgaaccacc gcgtttacac
    33121 caccacgggt ggcatcacgc agcgctttca cgccgggaat gtcacgcagc gtctgaataa
    33181 gcggcgtcag caccgcgcag tcgctgacca gttcgccatc cagccccagc tgctcacgca
    33241 ggttaaggat agtcgccccg tggtcgccga gtgtaccgct caccagcaat acatcgcctg
    33301 cggttagcgt ctgtgcgccc cagtgaatat tcgccggaat tgcgcccatg ccagcggtgt
    33361 tgataaacag tttatctacc gcgccgcgct gcaccacttt agtatcgcca gtaacgatgg
    33421 caatgcctgc cgtgcgggcg gtttttgcca tgctggtcac tacggctttc agtgtctcca
    33481 tcggcaatcc ttcttcgagg ataaagccac aggagagata gcgcggaata gcgccactga
    33541 ccgcaacgtc attcgcggtg ccgcaaatcg ccagcttgcc gatattaccg ccaggaaaga
    33601 acagcgggtc aatgacgtaa ctgtcggtgg agaacgccag acgatcgcct tctgctacca
    33661 gctgcgccag atcaagacgt gcctggtctt cctgctctgc cagccacggg ttggcaaagg
    33721 cttccataaa caggctgttg attaattgct gcatcgcctg gccgccgctg ccgtgggcga
    33781 gttggatatt attcacgctt cattctcctg ctgacgatac tgataccacg cggcgcacgc
    33841 tccttcggag gagaccatca gtgcaccaaa tgcggtttga ggattacagg tgttaccaaa
    33901 cagcgggcat tgatgcggct tacatttgcc cgttaatact tcaccacaac gtgcgcgcgg
    33961 gtcatcgcag acctgctgcg gtgccgggcg gaaatgtgct tcggcatcga atcgttgata
    34021 gtccggcgtc aggtgtacac cagaagattc aatcacgcct aagccgcgcc attcgctgtc
    34081 gccgttgacg cagaagacat ccgcaatcgc ctgttgcgcc agcaggttac cggcatccgg
    34141 cactacccgc cgatactgat tttctacctt gctatgagca gctattttct gctccaccag
    34201 catgaccacg ccttgtagta gatcaagggg ttcgaatcca gcaaccacca gcggacgatg
    34261 aaaatcgctg gcgataaaat tataggcgtt ggtgccgata accatactga cgtgacctgg
    34321 cgcgaggaac gcatcgatac cgttatccgg ctgttccagc aaactgcgca acgtcgggat
    34381 aagcgtaatg tgctggcaga agaagtaaaa attctgcaca tcacgcgctt tcgcctgttg
    34441 cagagtgatg gcggtggtcg gcatggtggt ttcaaaacct aagccgaaga acaccacttt
    34501 gcgggttgga ttctcctgcg ccagtttcaa cgcatccatt ggcgagtaaa cgatgcgaac
    34561 atcggcaccg cgtgcttttg cctgcaacag cgatccctgt ttccccggca cgcgcatggc
    34621 gtcgccaaag gtacagaaga tgacttccgg atggctggca atctccacgc aggtgtcgat
    34681 tctacccatc ggcagtacgc acaccgggca acccggaccg tggataaact caacgttttc
    34741 aggcagtaac tggtcgaggc cgaatttaaa aatggcgtgg gtatgaccac cgcacacttc
    34801 cataatccgc agagggcgtt cggctgtgta agagagatgt gaagcacgtt cgcgcagatg
    34861 ctcaattaac tgcatcacct gttccggcgc gcgatattca tcaacaaaac gcattatttt
    34921 tcctcgccat acaacagcgc gccgacatcc ggctcaacgt caaacatgtt ttgtaaggcg
    34981 tcgagagtgt cgcgtgcttc ggcttcatta attacgctca tggcaaagcc aacgtgtacc
    35041 agtacccact ggcccacgcg cggctgaccg ttttcatcgc agctgccgac taacgttaaa
    35101 tcgacatcgc gctgaatgcc gcagacgtcg actttcgcct ggttgccgtc aatggtgcgg
    35161 atctggccgg gaacgcctat gcacatcgct gtgtctccag ccagttcagc cactggtcca
    35221 tcccttcgcc gctggtggcg gaaataagga tgatttcaat ttctggattg acttcgcggg
    35281 cgcaggcgat gcacttctca acgtcaaagt tgagatacgg cagcaggtca actttgttga
    35341 gcagcatcag cgaggcggcg gcaaacatat gcggatattt cagcggtttg tcttcacctt
    35401 cggtaacgga aagcaccgcc actttgtgtt tttcaccgag atcgaagctg gccgggcata
    35461 cgaggttgcc aacgttttcg ataaacagaa taccgttatc gtccagtggc aggcgcggtg
    35521 cggcttcggc aatcatctgt gcgtcaagat ggcagccttt accggtattc acctgaatcg
    35581 ctggtgtgcc ggtagcgcga atgcgtgcgg catcgttcac ggtttgctga tcgccttcaa
    35641 taactgcgca cggaacgctg tctttcaggc gcattagggt ttccgtcagc agggtggttt
    35701 taccggaacc agggctggaa accaggttga gcaccagttg cttgcgggca gcaaagcgcg
    35761 cgcggttgcg ttcagccaga cggttatttt tgtccagcac gtcaatttcg acttccagca
    35821 tccgacgctg gctcatgccc ggcgcgtgag tgcctgcttc gccatgaccg taatgcaggt
    35881 cgccttcttc agtctggctg ggggtaaatt caggcgcttt aatgccggtg attttcatct
    35941 tcgggcgtgc cgccggggca aatggcgcgc tacgaaacgc ggaatgaggg ttatgttcat
    36001 caccctcgat atacaggttg ccttcaccgc aaccgcatgt tgtacacatc gctcactcct
    36061 ggtctatttc tatccgccga atctgtaaac cgtcgtctgc cacaatctgt agcatgtcac
    36121 catgacactg tggacagcgg cggacgcgct gggtcagtag cgtcacatac tgttggcatg
    36181 tttcacacca gcattcggcc tcttgttctt cgaggtgcag tttacaacct tccgccacgc
    36241 tgccgcggca aaccagatca aaacaaaagg caagagagct ggtttcgaca caagaaaatg
    36301 cgccaatttt gagccagacc ccagttacgc gttttgcgcc gtgttttgcg gcctgctgtt
    36361 cgatcaattc cagtgcccgt tggcagaggg ttatttcgtg catatcgcct cccattaact
    36421 atggccagct acaagcaata attgtgccag tattgattat ccctgcggtg aataatgtcg
    36481 atgatgtcga aatgacacgt cgacacggcg acgaaattca tctttagctt aaaaatcgtt
    36541 ttaataacaa tgaattaaaa gttggcacag aaaatgctta aagctggcat ctctgttaaa
    36601 cgggtaacct gacaatgact atttgggaaa taagcgagaa agccgattac atcgcacagc
    36661 ggcatcgtcg cctacaggac cagtggcaca tctactgcaa ttcgctggtt caggggatca
    36721 cgttatcgaa agcgcgcctg catcacgcca tgagctgcgc gccggacaaa gaactctgtt
    36781 tcgtcctttt tgaacatttt cgcatttacg tcaccctggc ggatggcttt aacagccaca
    36841 ccatcgagta ttacgtcgaa acaaaagatg gcgaagacaa acagcggatt gcgcaggcgc
    36901 aactgagcat tgacggcatg attgatggca aggtcaacat ccgcgatcgc gaacaggttc
    36961 tggaacacta tctcgaaaaa atcgctggcg tttacgacag cttatacacc gctattgaaa
    37021 acaatgtgcc ggtgaattta agccaactgg taaagggaca aagcccggca gcatgagctg
    37081 aggctttgcc cgttttgcag gcgttacgcc tgtttgggga tgggcgtgtc gatgagtgtc
    37141 gaaaatgaca tttcatcggc atgttttcgt caaaaatgac aatcacctga ggaatgcctg
    37201 gtgaatcgtt ttgtaattgc tgactccacg ctctgtatcg gctgccacac ttgtgaggcc
    37261 gcctgttcag agacgcatcg ccagcacggc ctgcaatcaa tgccgcgcct gagagtgatg
    37321 ctgaatgaaa aagaatctgc gccgcagctc tgtcaccact gtgaagatgc accctgcgcg
    37381 gtggtctgcc cggttaacgc catcacccgc gttgatgggg ccgtgcagtt gaatgaaagc
    37441 ctgtgcgtaa gctgcaagct gtgcggcatc gcctgcccgt ttggcgcaat tgaattttcc
    37501 ggcagccgtc cgctggatat tccggcaaac gccaataccc cgaaagcgcc accggcaccg
    37561 cctgctccgg cgcgtgtcag cacattgctt gactgggtgc caggtattcg cgcgatcgcc
    37621 gtcaaatgtg acctttgtag ctttgatgaa caaggtccgg cctgcgtgcg gatgtgcccg
    37681 actaaagccc tgcatctggt ggataacact gatatcgccc gcgtcagcaa acgtaagcgt
    37741 gagctgactt ttaacacgga ctttggcgat ctcaccttgt ttcagcaggc tcaaagtgga
    37801 gaggctaaat gagcgcaatt tccctgatca atagcggcgt ggcgtggttt gtcgccgccg
    37861 ctgttctggc atttctcttt tcttttcaaa aagcgttaag tggctggata gctggaattg
    37921 gcggcgcggt tggtagtctg tatacggcag ccgcgggctt cactgtactg actggcgcgg
    37981 ttggcgtgag cggtgcgctg tcgctggtaa gctacgatgt gcaaatctct ccgcttaacg
    38041 cgatttggct gattacgctc ggtctgtgcg gtctgtttgt cagcctctac aacattgact
    38101 ggcatcgcca cgcgcaggtg aagtgcaacg gcttgcagat caatatgttg atggctgccg
    38161 ccgtctgcgc cgtcattgcc agcaacctcg gcatgttcgt ggtaatggcc gaaatcatgg
    38221 ccctgtgcgc ggtgttcctc accagcaaca gcaaagaggg caaactgtgg tttgcgctgg
    38281 ggcgtcttgg cactctgctg ctggcgattg cttgctggct gctgtggcag cgttatggca
    38341 cgctggatct gcgcctgctg gatatgcgta tgcaacagct gccgctcggt tccgatatct
    38401 ggctgctcgg tgtgattggc tttggcctgc tggccgggat tattccgctg cacggctggg
    38461 tgccgcaggc acatgctaac gcctctgcgc cagctgccgc gttgttttct acggtagtca
    38521 tgaaaattgg cctgctgggc attttaaccc tgtcactgct gggcggtaat gcaccgctgt
    38581 ggtgggggat cgcgctgctg gtgctcggca tgatcaccgc gttcgtcggt ggtctgtatg
    38641 cgttgatgga acacaacatc cagcgcctgc tggcttacca caccctggaa aatatcggca
    38701 ttatcctgct ggggctgggc gctggcgtaa cgggtatcgc gctcgaacaa ccggcactga
    38761 ttgctcttgg cctggtcggt ggtctgtacc atctgcttaa ccatagcctg ttcaaaagcg
    38821 tactgttcct cggagcgggg agcgtctggt tccgtaccgg tcatcgcgat atcgaaaaac
    38881 tcggtggtat tggcaagaaa atgccggtta tctccatcgc catgttagtc gggctgatgg
    38941 caatggctgc gctgccgccg ctgaatggtt ttgccgggga atgggttatc tatcaatcct
    39001 tcttcaaact gagcaatagt ggcgcgtttg ttgcccgtct gctggggccg ctgctcgccg
    39061 tgggtctggc aattaccggt gcgctggcgg tgatgtgtat ggcgaaagtc tatggcgtca
    39121 cgttcctcgg cgcgccgcgt accaaagaag ccgaaaacgc cacctgtgca ccgctcctga
    39181 tgagcgtaag cgtagtggca ctggcgattt gctgcgtaat tggcggtgtt gctgcgccgt
    39241 ggctgctgcc gatgctgtct gctgctgtac ctctgccgct ggagcctgct aacaccaccg
    39301 tttctcaacc gatgatcacg ttgctgctga ttgcctgccc gctgctgcca tttatcatta
    39361 tggcgatttg caaaggcgat cgtttgccat cgcgttcccg cggcgcggcc tgggtgtgcg
    39421 gctacgacca cgaaaaatca atggtgatta ccgctcacgg ttttgccatg ccggtgaaac
    39481 aggcgcttgc gccagtgctg aaactgcgca aatggctgaa tccggtgtcg ctggtgccgg
    39541 gctggcagtg cgaaggtagt gcgttgctgt tccgccggat ggcgctggtt gaactggcgg
    39601 tactggtggt gattattgtt tcacgaggag cctgagaatg agtgttttat atccgttaat
    39661 tcaggcgctg gtgttatttg ccgttgcgcc gctgctctcc ggtattaccc gcgtggcgcg
    39721 cgcccgtctg cataaccgtc gtgggccggg tgtgttgcag gagtatcgtg acattttcaa
    39781 actgctgggc cgccagagcg ttggcccgga tgcctccggc tgggtgttcc gcctgacgcc
    39841 gtatgtgatg gtgggcgtca tgctgactat cgctactgcg ctgccggtgg tgaccgtcgg
    39901 ttctccgctg ccgcaactgg gtgatttaat caccttactg tatctctttg ccatcgcgcg
    39961 tttcttcttt gccatttcta gtctggatac cggtagcccg tttaccgcta tcggcgcgag
    40021 ccgtgaagcg atgcttggcg tgctggtcga accgatgctg ctgcttggtc tgtgggttgc
    40081 cgcacaggtt gccggttcca ccaacatcag caacatcacc gacaccgttt atcactggcc
    40141 gctgagccag agcatcccgc tggtgctggc gctttgtgcc tgtgcgttcg ccacttttat
    40201 cgaaatgggc aaactgccgt tcgacctggc ggaagccgag caggagttgc aggaaggtcc
    40261 gctctccgaa tacagcggta gcggctttgg cgtcatgaaa tggggtatca gcctgaaaca
    40321 gctggtggtg ttgcagatgt tcgtcggggt gtttattccg tggggacaaa tggaaacctt
    40381 caccgccggt ggactgctgc tggcgctggt gattgccatc gtcaaactgg tggtcggcgt
    40441 cctggttatc gcgctgttcg aaaacagcat ggcccgtctg cgtcttgata ttactccgcg
    40501 cattacctgg gctgggtttg gctttgcatt tttagcgttc gtctccttgc tggcggcgtg
    40561 attaaagaga gtttgagcat gtctgaagaa aaattaggtc aacattatct cgccgcgctg
    40621 aatgaggcat ttccgggcgt cgtgctggac cacgcctggc agaccaaaga tcagctgact
    40681 gtcaccgtaa aggtgaacta cctgccggaa gtggtggagt ttctttacta caaacagggt
    40741 ggctggctgt cggtgctgtt tggtaacgac gaacgcaaac tgaatggtca ttacgccgtt
    40801 tattacgtgc tgtcgatgga gaagggcact aagtgttggg taacggttcg cgtcgaagtt
    40861 gacgccaaca aaccggagta tccgtccgtg acgccgcgcg ttccggcggc tgtgtggggc
    40921 gagcgcgaag tgcgcgatat gtacggtttg attccggttg gtctgccgga tgaacgtcgt
    40981 ctggtgctgc cggatgactg gccggatgaa ctttatccgc tgcgtaaaga cagcatggat
    41041 tatcgtcagc gtccggcgcc gaccaccgat gctgaaacct acgagttcat caatgaactg
    41101 ggtgacaaga aaaacaacgt cgtgccgatt ggtccgttgc acgtcacttc cgacgaaccg
    41161 ggtcacttcc gtctgttcgt cgatggcgaa aatattatcg acgccgacta ccgcctgttc
    41221 tacgtccatc gcggcatgga aaaactggcg gaaacccgta tggggtacaa cgaagtgacc
    41281 ttcctctctg accgtgtgtg cggcatctgc ggctttgctc acagcaccgc ctacaccacg
    41341 tcggtggaaa acgcgatggg tattcaggtg ccagaacgtg cgcagatgat ccgcgccatt
    41401 ctgctggagg tggaacgcct gcactcgcat ctgctcaacc tcggcctggc ctgtcacttt
    41461 actggcttcg actccggctt tatgcagttc ttccgcgtgc gtgaaacctc catgaaaatg
    41521 gcagagatcc ttaccggtgc gcgtaaaacc tacggcctga acctgatcgg cgggattcgt
    41581 cgcgatctgc tgaaagatga catgatccag acccgccagc tggcgcaaca aatgcgtcgt
    41641 gaagtgcagg agctggtgga tgtgctgctg agtacgccga acatggaaca gcgcactgtc
    41701 ggcattggtc gtctggaccc ggaaatcgcc cgcgatttca gtaacgttgg cccgatggtc
    41761 cgcgccagcg gacacgctcg cgatacccgc gccgatcacc cgtttgttgg ttatggcctg
    41821 ctgccaatgg aagtccacag cgagcagggc tgcgacgtta tttcccgtct gaaagtgcgt
    41881 atcaacgaag tctataccgc gctgaacatg atcgactacg gtctggataa cctgccgggt
    41941 ggtccgctga tggtggaagg ctttacctac attccgcacc gtttcgcgct gggctttgcc
    42001 gaagcgccgc gcggcgacga tatccactgg agcatgaccg gcgacaacca gaagctgtac
    42061 cgctggcgct gccgtgccgc gacctacgcg aactggccga ccctgcgcta catgctgcgt
    42121 ggcaataccg tttccgatgc gccgctgatt atcggtagcc tggacccttg ctactcctgt
    42181 accgaccgca tgaccgtggt cgatgtgcgt aagaagaaga gcaaagtggt gccgtacaaa
    42241 gaactcgagc gttacagcat tgagcgtaaa aactcgccgc tgaaataagg aatcgccatg
    42301 tttaccttta tcaaaaaagt catcaaaacc ggcacggcga cctcgtctta tccgctggag
    42361 ccgattgcgg ttgataaaaa cttccgtggt aagccagagc agaacccgca gcagtgcatc
    42421 ggctgcgcgg cctgcgtcaa tgcctgcccg tcaaacgcct taacggttga aactgacctc
    42481 gccacaggag agcttgcgtg ggagtttaat ctcgggcgct gcatcttctg tggacgctgc
    42541 gaagaagtct gcccgacggc agcgatcaaa ctgtcgcaag agtacgaact ggcggtgtgg
    42601 aagaaagaag atttcctgca acagtcccgt tttgtgctgt gcagctgtcg cgtctgcaat
    42661 cgtcctttcg ccgtccagaa agagatcgac tacgccattg cgctgcttaa gcacaatggt
    42721 gacagccgcg cggaaaacca ccgcgaaagc tttgagactt gcccggaatg taagcgccag
    42781 aaatgcctgg tgccgtccga ccgtattgaa ctgactcgcc atatgaaaga ggccatctga
    42841 tgagcaattt attaggcccc cgtgacgcca acggcattcc ggtcccgatg acggtggatg
    42901 aatccatcgc cagcatgaag gcgtcgttac tgaaaaaaat caaacgttct gcctacgttt
    42961 accgcgtgga ctgcggcggc tgcaacggct gcgaaatcga aattttcgcc acgctttcgc
    43021 cgctgtttga tgcagaacgc ttcggcatta aagtcgttcc ttcaccgcgt catgcggata
    43081 ttttactgtt taccggcgcg gtcacccgtg caatgcgatc ccctgcgctg cgtgcgtggc
    43141 agtccgcgcc ggacccgaaa atctgtatct cctacggtgc ctgcggtaac agtggcggga
    43201 tcttccacga tctctactgc gtgtggggcg gtacggataa aatcgtcccg gtagatgtct
    43261 acatccctgg ttgcccgcca acgcctgccg ccacgctgta cggctttgca atggcgctcg
    43321 gcctgctgga gcagaaaatt cacgcccgtg gcccgggtga gcaggatgaa caaccggcgg
    43381 aaatcctgca tggcgatatg gtgcagccgc tgcgtgtgaa agtggatcgc gaagcacgcc
    43441 gcctggcagg ttatcgttac ggtcgtcaga ttgccgatga cttcctgaca cagttagggc
    43501 agggcgaaga acaggttgca cgctggctgg aagcggaaaa cgatccgcgt ctgaacgaga
    43561 ttgtcagcca tctgaatcat gttgtagaag aggcgcgtat ccgatgagtg aaaaggtggt
    43621 gttcagtcaa ctgagccgta aatttattga tgagaacgat gccacgcccg ccgaggcgca
    43681 gcaggtggtc tattacagcc tggcgattgg tcaccacctt ggggttatcg attgcctgga
    43741 agcggcgctc acctgcccgt gggatgaata tctggcatgg attgccactc tggaggcggg
    43801 cagcgaagcc cgccgcaaaa tggaaggcgt gccgaaatat ggtgagatcg tcatcgacat
    43861 taaccatgtg ccgatgctgg ccaacgcatt cgataaagcc cgggcagcgc aaacttcgca
    43921 gcagcaggaa tggagtacaa tgctgctaag tatgctgcat gatattcatc aggaaaacgc
    43981 catctatttg atggtgagga gactgcgtga ctgacgtttt actctgtgtt ggcaatagca
    44041 tgatgggcga tgatggcgca ggcccgctgc tggcggaaaa gtgcgccgcc gcgccgaaag
    44101 gtaactgggt ggtgattgac ggcggtagtg caccggaaaa cgacatcgtc gccatccgtg
    44161 aactgcgccc tacgcgactg ctgattgtcg acgccactga tatggggcta aaccccggcg
    44221 agatccgcat catcgacccg gatgatatcg ccgagatgtt tatgatgact acccataaca
    44281 tgccgttgaa ctaccttatc gaccagttga aagaagatat tggcgaagtg attttcctcg
    44341 gcatccagcc ggatatcgtc ggcttttact acccgatgac ccagccgatt aaagatgcgg
    44401 ttgaaaccgt ttatcaacga ctggaaggct gggaagggaa tggcggcttc gcgcagttag
    44461 cggtggaaga agaataattc tccatcaggg aattcagcga aatgaatatc agaacaggga
    44521 tgtgcgcttt tttattgagc ttagtgctgc ctgcacaggc caccagcttt accgaatatc
    44581 tgccaatgag tgacagcgaa tacgcgcaaa agcgggcgct gaaacctctc ctgaccatgc
    44641 cttatgatgc cgagcaaacc tggcatttta gaaaagtggg cgttgctggc gtgacgcttg
    44701 aaaagatgcc aaatgatgat agtgaatggc aattgaacgg taaagatcgc gccggtaaat
    44761 cctggtctgt gcctgtgggt atgctggcaa acatagcggg caaagggcaa ctctatcgcg
    44821 ccgatcttga tcgtaacggc attcaggatc tggttatctg gttaccaagt accggtaatg
    44881 gtctggctcc ttatgcacat ttaattctta tgacgttcac gcgcgaaggc cgtccctgtg
    44941 tctttgaacc caggggattc tacaccgcaa gcaaaactgg cgtagatgat ttgctggatt
    45001 tgcaaggcaa tggtcacacg caattgctgg atatgcagtt taattctggt tactggataa
    45061 ccagtctgta tcaggtaaaa gacgccaaat ggcagcgtgt tcacggctgg tttggcaagt
    45121 taagctatcc cgcgctcacg cgttttactt atacccccaa ccgtaaactc gtcctcaaac
    45181 ccatcgccgg gcgcgacccg caaacggaag atctggcgca gacgcagcgt tgcttgataa
    45241 agggggatgt gcttgagggg tgaaccagaa ctgacttttt gcagagtggc tatgaggatt
    45301 ttgggggtaa ttatatcgcg cacaagtgtt tacatattgt gaacttggat ttgatgagct
    45361 atagtttccg aacacataag gatagaacat cgcagatgtg gctgtttact tactatcagg
    45421 aagttaaaac ttaaaaatgc acatcatttc taaagcgcca tttgaagagt ctgcgcgtaa
    45481 atatcctaac gacgctctgg ctcttcaggc attgtatcgg gttattaagg aaacggattt
    45541 ttctacacca gaagaaatgc gaacggcgtt tcccaacctc gacaatttta gatatcgcaa
    45601 caaatggtac gttctggatg tcggcgggaa caatcttagg gttattgcct acataaactt
    45661 tgttaataag cggttctttg tgaagcacat aacgaatcac gcagagtatg acaaactgac
    45721 acgttactac agagagaaca aagaatgact gctaatgctg cacgcgctgt taaagcaaca
    45781 agagaactgg tcaatgcagt cccctttctg ggaggcagtg actcagaaga tgattatcgt
    45841 gaagcactgg aactggtcga atatttgatt gaagaagatg acaccaatcc acttatcgac
    45901 tttctggcga gccgcattgc agagtatgaa aacaacaacg aaaagtttgc agagttcgac
    45961 aaagccgttg cggcaatgcc ggttggcgtg gcgttacttc gcacgttgat tgatcaacat
    46021 aatctgacct atgcggattt gaagaacgaa atcggctcta aatccctggt cagtcagatt
    46081 ttgtctggac aacgatccct gacgatatcc cacattaagg cactttcagc ccgatttggc
    46141 gttaagccgg aatggtttct ttgattttga ctgatgccaa accccagatt gcccgatgcg
    46201 ctgcgcttat cgggccttcc tggttcgtgc gacatgtagg ccggataagg cgttcacgcc
    46261 gcatccggca ctgttgctta ctctaaatct tcaccattac tggcaatcac ttttttatac
    46321 caccagaacg atttcttacg tgtgcgcgtc agcgtgccgt tgcctgcgtc gtcacggtct
    46381 acgtagacaa aaccgtagcg tttgctcatt tcacccgtag aggcggaaat taaatcaata
    46441 cagccccatg tggtgtagcc catcagcgga atgccatctg caatcgcttc gcccattgcg
    46501 cggatatgtt cgcgtaaata gctgatgcga tagtcatcgt taatctcgcc attggcagca
    46561 agttcatctt ttgcgccgag gccgttttcc accagaaaca gcggcttctg ataacggtcg
    46621 tacatcatgt tcatggtgat acgcagaccg agtggatcaa taccccagcc ccagtcgctc
    46681 acttgcagat acgggttacg cagcgatttc actacgttcg ccgcactgct attgttggcg
    46741 ttcatctccg ccgaggcgca gcgcgaggcg taatagctga aagagacaaa atcgacggtg
    46801 tttttcagga tttcatcatc gcccggtgct ttgtcgatgg ttaccccttt ttcgcggaat
    46861 acgcgggcag agtaagccgg atacgcgccc cgcgcctgca catcgataaa aaacaggttt
    46921 tcccggtctt tctccagcgc cgcccagaca tcttccggct tgcaactgta agggtagaag
    46981 ttaccgcccg ccagcataca gcccacctga ttttgcgggt taacctcatg ggcgattttg
    47041 gtggctagcg cactggcaac cagctggtga tgcgcggcct gatatttcac ctgatcctga
    47101 ttttcacctt cttcaaacac cagacccgcg ccggagaacg ggctatgcaa cataatgttg
    47161 atttcattga aggtaagcca gtatttcacc agaccatcaa atgcttcaaa gcaggtccgg
    47221 gcgtagcggc tgaaaaactc caccagcttg cggttacgcc aggagccata ttcggtgacc
    47281 agatgcatcg gcacatcgaa gtggcacaac gtgaccagcg gttcgatacc gtacttttta
    47341 cactcttcaa agacagaacg ataaaaagca atgccctgct gattgggcgt gatttcatcg
    47401 ccctgcggaa agagacggct ccaggcaatt gaggtacgga aaaccttgaa tcccatctct
    47461 gccatcaggg cgatatcttc tttataacga tgataaaaat ccgtcgcctc atggctggga
    47521 taaaactcgt catcgcgcaa ctgaaaacgt ttttccagcc ccagtttcac cgccattcga
    47581 tgctcgccgt gtgggatcat atcgacagtg gtcagccctt tgccaccttc acggaacgca
    47641 ccttcagact ggttggcggc aagcgcgccg ccccataaaa aaccttctgg aaatactgac
    47701 attttcatcc tcaattaaga cttacttctt tggcgacggt cggttgtacg ctctgatgct
    47761 ttcgcgcctc ggcagtcgct tcctcaacag gaatatcctc gaagccgagc aacagtgtga
    47821 ggataaacga cagcatgacc gccagcgcca tgaccgcgaa cacccagacg atgctcattg
    47881 gattcgccgg atcgaagaac tgcacgctgg taaatagccc cggcgctgcc attgagtggc
    47941 tggcaagccc cgccataccg gcaaccgcgc cgcaaataaa gccgctgata agactggcga
    48001 taagcggacg tttcaggcgg atcgccacgc cgtataacgc cggttcggaa atccccgcca
    48061 taatggctga tgccgccgca gccagcgccg tctggcgcag ttccgggttt ttcgttttcc
    48121 acgccaccgc cagtgatgaa ccgcccagcg acaggttagc gccgatctct gacggcatga
    48181 ccatcccttc tttgccggtt tcggcaatgg tctgaatgat ggttggcgta aagacgcggt
    48241 gcatcccggt cattaccagc agaggccaca gcgcgcccat aatggcgact gaaagccagc
    48301 ccagataacc atgaatggtg taaaccagcg ccgaaatggc gctaccgatc cagataccaa
    48361 tcgggccaat cagcaggatt gccagcggtg cggcaatcaa cacaatcagc attggcttga
    48421 ggaagttttt ggtcaccgcc ggtgtaatgc gatccaccca gcgttcgata tatgacaggc
    48481 accaggtcat caccagcgcc gggatcactg tgtaggtgta tttcaccgcg gtgaccggaa
    48541 tcagggcaaa ttcgacatgt tcaccctggg ccgctttcgc catcagttca ataaaactcg
    48601 gatgtaccag cacacccgcg atggcaatcg ccagcgacat attggtttta aatttgatgg
    48661 cggcagatgc cgcgaccatc agcggcagga agaagaaagc accgtcacca atcacattca
    48721 gaatggttaa ggtcggcgat ccttttgtca gcacgccgct catctcgagg atcatcgcca
    48781 gcagtttgac catcgatccg ccgataatcg ccgggatcag cggtgacatg gtgccgatca
    48841 gcgcatcgag gatccctgca ccaatgcgac gcagcgtgag tttgggttta cccacgggct
    48901 gtgcgggctg catatctccc ggcagcaggc tgacgacttc ctgaaaggct tgtgaaacgg
    48961 tattgccgat aatcacctga cactggttgt cactacgtac cacgccgagc acgccgggga
    49021 tggtttttaa cgtcgggctg tcgataagct ggtcatcttt gataacaaag cgcaaacgcg
    49081 tcatacagtg cgtgaccgcc gagatgttat caacgccgcc cagtgccgct atcaccgagc
    49141 gtgccagcgc cgcataattt ttggccatcg gattttatcc tgttatcagt agggtagatg
    49201 tagttgttat cagtcgacgt aacggaaaaa tgccgatgct gaaacggaat taggaaaccg
    49261 gtttcacaaa atcatgaata gttgtcacta aataagcaag agagttaatt gttcattttt
    49321 tatatttgtg aaaccggtca cctgaatatt tttcccgccg attgcgcaag gacaatcggc
    49381 aaacggtgca gtacactgcg ttgattgcgt gtaaagggat gaaaatgatg acgacgatgc
    49441 tggaagtggc gaagcgcgcc ggggtttcaa aagcgaccgt ttcccgcgtg ctttcaggta
    49501 atggctacgt cagccaggag actaaagatc gcgtgtttca ggcggtagaa gagagcggtt
    49561 accgcccaaa tttgctggcc cgcaatctgt cggcgaagag tactcagacg ctggggctgg
    49621 tagtgaccaa cacgctttat cacggcattt attttagtga attactgttt catgccgcgc
    49681 gaatggcgga agagaaaggg cggcagttgc tattggcaga tggtaaacac agcgccgaag
    49741 aagagcgcca ggcgattcag tatctgctgg atttgcgctg cgacgccatt atgatttacc
    49801 cgcgtttttt aagcgtggat gagattgatg acatcattga cgcgcacagt cagccgataa
    49861 tggtgcttaa tcgccgcctg cgcaaaaaca gcagccatag cgtctggtgc gatcataaac
    49921 agaccagctt taatgccgtg gcagagttga taaacgccgg acatcaggag attgctttcc
    49981 ttaccggctc gatggattcc cccaccagcg ttgaacgtct tgccgggtat aaagacgcgc
    50041 tgtcgcagca tggtattgcg ctcaatgaaa aacttatcgc taacggtaaa tggacgcccg
    50101 ccagcggggc cgaaggggta gaaacgttgc tcgaacgtgg ggctaaattt agcgcgttag
    50161 ttgccagtaa cgacgatatg gcgataggtg cgatcaaagc gttgcacgag cgcggcgtag
    50221 cggtgccaga gcaggtgtcg gttatcggat tcgatgatat cgcaattgcc ccctacaccg
    50281 ttccggcgct ctccagcgta aaaattccgg taacagagat gattcaggaa attattggac
    50341 ggctgatttt tatgctcgat ggcggggatt tctcaccgcc gaaaaccttc agcggaaaac
    50401 tgatccgccg cgactcactc attgcccttt cgcgataaac attgtcatca catgtcatcg
    50461 tcactagtgt cgatgacatc ttttttcctg cattacagaa attaatttcc ttaaataaca
    50521 gcaaattaaa aactggcatg atttgtgaat gtatcggcgc attaactgtc attgctggag
    50581 aatttgatga accgtttcat cattgctgac gcgagtaaat gtattggttg ccgtacctgt
    50641 gaagtggcgt gcgtggtttc tcatcaggaa aatcaggact gtgcatcgct gaccccggaa
    50701 acctttttac cgcgaatcca tgtcattaaa ggtgtgaaca tttccacggc gacagtctgc
    50761 cgtcagtgtg aagatgcacc gtgcgctaac gtctgcccga atggtgctat cagccgtgat
    50821 aaagggtttg ttcatgtgat gcaggaacgt tgcattggtt gcaaaacctg cgtggtggct
    50881 tgcccgtatg gcgcgatgga agtggtggta cgtccggtga tccgcaacag cggcgcgggg
    50941 ctgaatgtac gggctgacaa agctgaagcc aataaatgcg acctgtgcaa ccatcgcgaa
    51001 gacggcccgg cgtgtatggc ggcttgcccg acccatgcgc tgatttgtgt cgatcgtaat
    51061 aaacttgaac aactgagcgc agaaaaacgt cgccgtacgg cgctgatgtt ctaaaaatct
    51121 tatagaactg ttctggcatc aaaggaattc ccggaaatat tcgtagaata cggagctggc
    51181 ggtatcgccg acctgagact gccggatacg acgcagtcgt gtccggcagg gcgggttacg
    51241 caattcatga tggcgggaat aactcaatgg caaaaaacac atcttgcggt gtccaactgc
    51301 gtattcgagg caaagtgcag ggcgtcggtt ttcgtccgtt tgtctggcag ctggcacagc
    51361 aattaaatct tcacggcgat gtctgtaatg acggcgatgg cgtagaagtc cggctgctgg
    51421 aagacccgga aacgtttctt gttcaattgc atcagcactg cccgccgctg gcgcgtattg
    51481 atagcgtcga gcgtgagccg tacatctggt cacaactgcc caccgagttc actatacgcc
    51541 agagcacagg cggcaccatg aatacgcaaa ttgttcccga tgccgctact tgccctgcct
    51601 gtcttgccga aatgaatacc ccaggcgaac ggcgttatcg ttatccgttt atcaactgta
    51661 cccactgcgg cccgcgtttc accattattc gcgccatgcc ttacgaccgc ccgtttaccg
    51721 tgatggcggc gtttccgttg tgtccggctt gtgacaaaga gtaccgcgac ccgctcgatc
    51781 gtcgcttcca cgcccagccg gtggcctgcc cggagtgtgg cccacatctt gaatgggtaa
    51841 gtcatggtga acatgcagaa caagaggcgg cattacaggc ggctatcgca cagttaaaaa
    51901 tgggcaacat tgtcgccatc aaagggattg gcggatttca tcttgcctgc gatgcacgta
    51961 acagtaacgc ggtggcgaca cttcgggcgc gcaaacatcg cccggcgaaa ccgctggcgg
    52021 tcatgttgcc agtggctgac ggtttaccag acgctgcgcg ccagttgctt accacgcccg
    52081 ccgcgccgat tgtgctggcg gataaaaaat acgttcctga gctttgtgat gatatcgccc
    52141 ctgaccttaa cgaagtcggg gtaatgttgc ctgcgaaccc gctccagcat ttgctgttac
    52201 aggaactgca atgcccgctg gtgatgacct ccggcaacct gagcggtaaa ccaccagcta
    52261 tcagcaacga acaggcgctg gcggatttgc agggcattgc cgacggattc ttaatacata
    52321 accgcgacat cgtgcagcgg atggatgatt cagtggtgcg cgaaagcggc gaaatgctgc
    52381 gccgttcgcg ggggtatgtg ccggatgcgc tggctttgcc tccgggcttt aaaaatgttc
    52441 cgcctgtgct gtgtctcggc gcggatctga aaaatacctt ctgcctggtg cgcggtgagc
    52501 aagcggtgtt gagtcagcat ctgggcgatt taagtgacga tggcatccag atgcagtggc
    52561 gcgaagcgtt acgcctgatg caaaacatct acgattttac cccgcaatac gttgtgcatg
    52621 acgcgcatcc gggctatgtc tccagccagt gggcgcgcga aatgaatctg ccgacgcaaa
    52681 cggtgctgca tcatcatgcc cacgcagcgg cgtgtctggc agagcatcag tggccgctgg
    52741 atggcggtga tgtcattgct ttgacgctcg acggtatcgg tatgggggag aacggcgctt
    52801 tgtggggcgg cgagtgcctg cgggtgaact atcgcgaatg ccagcacctg ggcggcttgc
    52861 ctgcagtggc gcttccgggt ggcgatttgg cggcgaagca gccgtggcga aacctgctgg
    52921 cgcagtgcct gcgctttgtg ccggagtggc agaattaccc tgaaacagca agtgtgcaac
    52981 agcaaaactg gagcgtgctg gcgcgggcca ttgagcgtgg aattaacgcg ccgctggcgt
    53041 catcgtgtgg gcggttgttc gatgcagtgg cggcggcact gggctgtgcg ccagccacgt
    53101 taagttatga aggtgaagcg gcttgtgctc tggaggcgct cgcagcctca tgccacggag
    53161 tgacgcatcc ggtgacgatg ccgctggtgg acaatcaact ggatctcgcc gctttctggc
    53221 agcagtggct gaactggcag gcaccggtta atcaacgcgc gtgggcgttt catgatgcgc
    53281 tggcgcaggg ttttgccgcg ttgatgcgtg agcaggccac gatgcgtggt atcactacgc
    53341 tggtatttag cggcggggtt attcataacc gtttgctgcg tgcacgtctg gcgcattatc
    53401 tcgctgattt cacattgctc tttccacaga gtttaccggc gggtgatggc ggtttgtctc
    53461 tggggcaggg ggttattgct gcggcgcgtt ggttagcggg tgaagtccag aacggataaa
    53521 acgtgttgca tttggacacc attgccggat gcggcgtaaa cgccttatcc ggcctacaca
    53581 tgcgtccccg taggtcggat aagacgcgcc agcgtcgcat ccgacatttt aggcacagta
    53641 gcccacctac atcggcaatg ttttcaacaa tccaaatgcc tctttcatcc gatcctcact
    53701 gaccacaaag gcgcgaagct ggtcagcatc gtcaacaccg cgagccacca ttccctggcg
    53761 ttcggtatta atttgccagc gtaaatcctg acgctgggtt tcgcctgcca gatgcagcgg
    53821 taattccggc gttttgattt tcacaagcat cgccggtagt ttcagcggcg tgttattgcc
    53881 gagaagattt tttgccagca ccatcgcgct aagttgaatc ggctgcagga atggcagtac
    53941 ctgaccgttg atttccgcgc aatcgcccag cgcataaata tcgtcattac tggtttgcag
    54001 ataactatcg acgcaaacgc cgcgattaat cgtcagcccg gcgcgtcgtg ccagggcggt
    54061 ttccgggcgc agtccggtgg cggcaattac cgcatccact tcgatgctac gctggcggtc
    54121 cagcgttgcc agaatgccag aatccgtttt ttccagcccc tgtaactgag atttcaacag
    54181 cagatgaacg cccatctccg tcaaccgatg ctgcaagcgg ctgcttactt ccggtggcat
    54241 taacgacgcc agaatactgg cagcgttgtc gattagcgtg accactttgc ctgcacgaca
    54301 aaaatccatc gccagttcgc taccaatcaa accaccgcca acaatcaaca cgcgtcgggc
    54361 atcccgcagt tgcgtttcac aggcgcgata ctcttgctga ctatttaacg tcagcattaa
    54421 ctcacgccca ggcacaggcg ggacaaaggc actggcaccg gttgccagta ctagcttgtc
    54481 gtattgccac tgattattct ggcttttcac cacacgggct tcggcatcga tatccgtcac
    54541 ccaggtttgt ggaaacaggt gcagattaaa ctgctcggca aattcacccg ccgtctggcg
    54601 ggtaaggtca tcggcacgtt gcgcctgact gataacatgg ctgaggtcag gtttgttgta
    54661 ctcatccatg ctgtcggcgg caatcagggt taatggaata gtggcgtcct gtttgcgaat
    54721 atttttcacc agttggcggg cggcgaagcc cgaaccgatg atcacaatgc cgttactcat
    54781 tttgcctccg atgccagttc gtcaaagacg tctttgccga gggagcattc cgggcagagg
    54841 aagttatccg ggacttcact ccacggcgtt cctggcgcaa cgtcctgcat tggctcgcct
    54901 tttgccggat cgtaaatcca ctggcagacg ctgcactgca ttcgtgggcc gaggtcagcc
    54961 gtcgtcgtgg cagaggtttc ttctttaact accgtattca ccgtgctctg cggcagcggc
    55021 gcgagcgccc actgacgggc gatttcgcga ccgtgttcac ggcataactc cagagcgtcc
    55081 tggtctggtc gccatttggc tttcaggctg agcgacattt cgaaacccgc atcctgcagg
    55141 cgcgtggaaa ggcgatccac cgcaccgccg ctccagccgt gagagccgaa agcactggcg
    55201 cgtttgttac ggaagcgtaa accagtcatc tcctccacca gcccggcgat tttcggcatc
    55261 atcacgttat tcatcgtcga agtgccgacc agcacgcctt ttgagcggaa gacattagtc
    55321 aggatttcgt ttttatcgct tcgggcgacg ttgaaaattt tcaccgccac gcgtgggtcg
    55381 gtttccgcaa tcccctgggc gatagcgtca gccatcatgc gggtgttatt cgacatggtg
    55441 tcgtagaaaa tggtgattct gtcttcctga taatcagccg cccatttcag gtacagctcg
    55501 acaatttgcg tcgggttatc gcgccatacc acgccgtggg aagtggctat catatcgact
    55561 ggtaagttaa agcccaggat ctcggtaatt ttcggcgtta ccaggcggct gaacggcgtc
    55621 aggatattgg cgtagtaacg ctggcactgc tcgaaaagct ccgtctgatc cacttcatcg
    55681 ttgaacagat gctcgtcgca gtagtgctga ccgaaagcat cgttactgaa cagcaccgcg
    55741 tcgcctgtca ggtaagtcat catgctgtcc ggccagtgca gcattggtgt ttcgacaaaa
    55801 atgagctgtt tgccgttgcc gatatccagc gtgtcgccag ttttcaccac attaaaattc
    55861 cactccggat gatggtgatg accatttatc gagtcgatag cgttggcagt acagtaaatc
    55921 ggcgtatcgg gaatttgcgc catcagttcg gtcagcgccc cggcgtggtc ctcttctgca
    55981 tggttaatca cgatgtaatc gatatccgcc agatcgattt cattacgcag gttctgcaca
    56041 aattcgcggc tgaatttatg gtcgacggtg tcgatcagca cgtttttttc ttcgcggatg
    56101 aggtagctgt tgtagctgct gccgcgcagc gttttatatt ccgtgccgtg aaaatcacgc
    56161 acttcccagt cacgttgacc cacccaatga atgttatttt tcaccacaat agacatagca
    56221 acctcaattt attcagcgtg ttctaaaaag atgtcttgct aattgcagat t
//
LOCUS       DAJOWB010000017        53030 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJOWB010000017 DAJOWB010000000
VERSION     DAJOWB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.017"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(50..262)
                     /gene="cspA"
                     /locus_tag="OHO85_001179"
     CDS             complement(50..262)
                     /gene="cspA"
                     /locus_tag="OHO85_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCQ2670592.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(543..833)
                     /locus_tag="OHO85_001180"
     CDS             complement(543..833)
                     /locus_tag="OHO85_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCQ2670593.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1268..1978
                     /locus_tag="OHO85_001181"
     CDS             1268..1978
                     /locus_tag="OHO85_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCQ2670594.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2028..3002)
                     /gene="ghrB"
                     /locus_tag="OHO85_001182"
     CDS             complement(2028..3002)
                     /gene="ghrB"
                     /locus_tag="OHO85_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCQ2670595.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3106..3765)
                     /locus_tag="OHO85_001183"
     CDS             complement(3106..3765)
                     /locus_tag="OHO85_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCQ2670596.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3919..6252
                     /gene="bisC"
                     /locus_tag="OHO85_001184"
     CDS             3919..6252
                     /gene="bisC"
                     /locus_tag="OHO85_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCQ2670597.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6221..6661)
                     /locus_tag="OHO85_001185"
     CDS             complement(6221..6661)
                     /locus_tag="OHO85_001185"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCQ2670598.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6658..7221)
                     /gene="tag"
                     /locus_tag="OHO85_001186"
     CDS             complement(6658..7221)
                     /gene="tag"
                     /locus_tag="OHO85_001186"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCQ2670599.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7379..8077
                     /locus_tag="OHO85_001187"
     CDS             7379..8077
                     /locus_tag="OHO85_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCQ2670600.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8306..9514
                     /locus_tag="OHO85_001188"
     CDS             8306..9514
                     /locus_tag="OHO85_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2670601.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            9838..11529
                     /gene="eptB"
                     /locus_tag="OHO85_001189"
     CDS             9838..11529
                     /gene="eptB"
                     /locus_tag="OHO85_001189"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCQ2670602.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11621..11697
                     /locus_tag="OHO85_001190"
     tRNA            11621..11697
                     /locus_tag="OHO85_001190"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11655..11657,aa:Pro,seq:cgg)
     gene            11732..11865
                     /locus_tag="OHO85_001191"
     ncRNA           11732..11865
                     /ncRNA_class="other"
                     /locus_tag="OHO85_001191"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            12611..14218
                     /gene="dppA"
                     /locus_tag="OHO85_001192"
     CDS             12611..14218
                     /gene="dppA"
                     /locus_tag="OHO85_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCQ2670603.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14526..15545
                     /gene="dppB"
                     /locus_tag="OHO85_001193"
     CDS             14526..15545
                     /gene="dppB"
                     /locus_tag="OHO85_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCQ2670604.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15555..16457
                     /gene="dppC"
                     /locus_tag="OHO85_001194"
     CDS             15555..16457
                     /gene="dppC"
                     /locus_tag="OHO85_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCQ2670605.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16468..17451
                     /gene="dppD"
                     /locus_tag="OHO85_001195"
     CDS             16468..17451
                     /gene="dppD"
                     /locus_tag="OHO85_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCQ2670606.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17448..18452
                     /gene="dppF"
                     /locus_tag="OHO85_001196"
     CDS             17448..18452
                     /gene="dppF"
                     /locus_tag="OHO85_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCQ2670607.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALGGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSFSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18482..19753)
                     /locus_tag="OHO85_001197"
     CDS             complement(18482..19753)
                     /locus_tag="OHO85_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCQ2670608.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20229..20336
                     /locus_tag="OHO85_001198"
     CDS             20229..20336
                     /locus_tag="OHO85_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCQ2670609.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(20423..22102)
                     /gene="bcsG"
                     /locus_tag="OHO85_001199"
     CDS             complement(20423..22102)
                     /gene="bcsG"
                     /locus_tag="OHO85_001199"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCQ2670610.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(22099..22290)
                     /gene="bcsF"
                     /locus_tag="OHO85_001200"
     CDS             complement(22099..22290)
                     /gene="bcsF"
                     /locus_tag="OHO85_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCQ2670611.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(22287..23858)
                     /gene="bcsE"
                     /locus_tag="OHO85_001201"
     CDS             complement(22287..23858)
                     /gene="bcsE"
                     /locus_tag="OHO85_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCQ2670612.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            24131..24319
                     /locus_tag="OHO85_001202"
     CDS             24131..24319
                     /locus_tag="OHO85_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCQ2670613.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            24331..25083
                     /gene="bcsQ"
                     /locus_tag="OHO85_001203"
     CDS             24331..25083
                     /gene="bcsQ"
                     /locus_tag="OHO85_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCQ2670614.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            25080..27698
                     /gene="bcsA"
                     /locus_tag="OHO85_001204"
     CDS             25080..27698
                     /gene="bcsA"
                     /locus_tag="OHO85_001204"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCQ2670615.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            27709..30048
                     /gene="bcsB"
                     /locus_tag="OHO85_001205"
     CDS             27709..30048
                     /gene="bcsB"
                     /locus_tag="OHO85_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCQ2670616.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            30055..31161
                     /gene="bcsZ"
                     /locus_tag="OHO85_001206"
     CDS             30055..31161
                     /gene="bcsZ"
                     /locus_tag="OHO85_001206"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCQ2670617.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            31143..34616
                     /gene="bcsC"
                     /locus_tag="OHO85_001207"
     CDS             31143..34616
                     /gene="bcsC"
                     /locus_tag="OHO85_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HCQ2670618.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            34698..36686
                     /gene="hmsP"
                     /locus_tag="OHO85_001208"
     CDS             34698..36686
                     /gene="hmsP"
                     /locus_tag="OHO85_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCQ2670619.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            36869..38155
                     /gene="dctA"
                     /locus_tag="OHO85_001209"
     CDS             36869..38155
                     /gene="dctA"
                     /locus_tag="OHO85_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCQ2670620.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            38376..39872
                     /locus_tag="OHO85_001210"
     CDS             38376..39872
                     /locus_tag="OHO85_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCQ2670621.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(39968..40897)
                     /gene="kdgK"
                     /locus_tag="OHO85_001211"
     CDS             complement(39968..40897)
                     /gene="kdgK"
                     /locus_tag="OHO85_001211"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCQ2670622.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            41129..41896
                     /gene="pdeH"
                     /locus_tag="OHO85_001212"
     CDS             41129..41896
                     /gene="pdeH"
                     /locus_tag="OHO85_001212"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCQ2670623.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVAHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            41966..44026
                     /locus_tag="OHO85_001213"
     CDS             41966..44026
                     /locus_tag="OHO85_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCQ2670624.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(44260..45582)
                     /locus_tag="OHO85_001214"
     CDS             complement(44260..45582)
                     /locus_tag="OHO85_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCQ2670625.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(45993..47006)
                     /gene="yhjD"
                     /locus_tag="OHO85_001215"
     CDS             complement(45993..47006)
                     /gene="yhjD"
                     /locus_tag="OHO85_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCQ2670626.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(47055..47954)
                     /locus_tag="OHO85_001216"
     CDS             complement(47055..47954)
                     /locus_tag="OHO85_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2670627.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            48474..49076
                     /locus_tag="OHO85_001217"
     CDS             48474..49076
                     /locus_tag="OHO85_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCQ2670628.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(49127..50776)
                     /locus_tag="OHO85_001218"
     CDS             complement(49127..50776)
                     /locus_tag="OHO85_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCQ2670629.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            51181..52578
                     /gene="ccp"
                     /locus_tag="OHO85_001219"
     CDS             51181..52578
                     /gene="ccp"
                     /locus_tag="OHO85_001219"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCQ2670630.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            52789..>53030
                     /locus_tag="OHO85_001220"
     CDS             52789..>53030
                     /locus_tag="OHO85_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCQ2670631.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT        12640 a        13290 c        14330 g        12770 t
ORIGIN      
        1 atccccgcca aatggcaggg atcttagatt ctgtgctttt aagcagagat tacaggctgg
       61 ttacgttacc agctgccggg cctttagcgc cgctttcgat ggtgaaggac actttctgac
      121 cttcgtccag agatttgtaa ccatcgttct ggatagcaga gaagtgtacg aacacgtctt
      181 tagagccatc gtcaggagtg atgaagccga agcctttgtc agcgttgaac cattttacga
      241 taccagtcat tttaccggac atagtgtatt acctttaata attaagtgtg cctttcggcg
      301 atatggcgtg ctttacagat tttgaagcgt taaaggaatg tgcactacga ggggtatcaa
      361 cgataactct tgaagggact tgccttacta cactgcttta atggtctgta cgtcaaaccg
      421 ttgatgtgca ttaagccacg cattggcggg tgatgcaaca attatttttc atatttatga
      481 ttaatcggtt ggaaaacggt cctgtcatca ggaccgtaaa cagcaataaa gtggataaaa
      541 gtctattcca tcaactgctt acttaatgcc gggttggctt gaatcaaacg catcaatttt
      601 agttcggcac ttgaaggctt cacgcgtctg gattcccatt cctttaccat ggcgactgat
      661 acgcccaaaa cccgggcgaa atcatcgatt tttaatcctg tcccttttcg taactgctca
      721 atttcggtac aggacgttgt tctgtgcgtc agggtaattt tctgcgtttc atctttaaaa
      781 acaatctgtt ccaggctgct caacagctca tgcattggat ctttatattc cattgaaaac
      841 tcctcaaatc acactgcggg atcgtgaatt gcatcgaagc tcattaagaa tagtcgggaa
      901 aagcagacac agcggggtga agtcagggca tgctctggct atgattcgat tcagccagtc
      961 atttagcgtg acttatagcc acctaacgat ctgataaatg ccgatatatc tcttaccact
     1021 cctggtttgt aaagatttcg aaaatctaac cttctgcgaa ggcgtaaatc tctggctaca
     1081 aaaaaagcaa cggcactatc atttttttaa caatgaaaca acaaattaac cttgtttcat
     1141 tttctccatg attttaccag gaagattacg tagaaagggt atcctgcaag cctgtcctgg
     1201 actatccttg tcacgtcaga cacgcgtgtg tcgttgtgcg ctttttttgg gtgaaaggag
     1261 taagaaaatg gcgacaggaa agtcctgctc tcgctggttt gcgcctcttg cggcgttatt
     1321 aatggtagtt agcctgagtg ggtgttttga taaagaaggc gatcagcgta aagcgtttat
     1381 cgacttcctg cagaatacag tgatgcgtag cggtgaacgt ctaccaaccc tgactgccga
     1441 tcagaaaaaa cagtttggtc cttttgtctc tgattacgcg attctgtatg gttattctca
     1501 gcaggtgaat caggcgatgg attccggtct gcgcccggtc gtagacagcg ttaacgcaat
     1561 tcgcgtaccg caggattacg ttacgcaaag cggtccgctg cgtgaaatga acggttcgct
     1621 gggtgtgctg gcgcaacaac tgcaaaatgc gaagttgcag gccgatgctg cgcactctgc
     1681 gctaaaacag accgatgatt tgaaaccggt ctttgatcag gcattcacca aagtggtaac
     1741 gacgccagct gatgcattgc aaccgttgat tccggctgca caaactttca cgcaacaact
     1801 ggtgatggtt ggagactaca ttgctcagca aggtactcag gtgagctttg tcgcaaatgg
     1861 catccagttc ccgacttcac agcaggcaag tgaatataac aaactgattg cgccattacc
     1921 ggcacaacat caggcgttta atcaggcctg gactacagca gttactgcaa cccaataaag
     1981 agtaaaagcc cgagcgatta tcgggcttta cttcacgcag tcgcggctta gtccgcgacg
     2041 tgcggattca cacagttctt ctcaaccttt ccttgtaacg catcaatcaa attatccacg
     2101 gcacaggcgg ccatgccata acgcgtctca tgggtggcag atccaatatg cggtactgcg
     2161 acgacgttgg ccattgagag caacggcgaa tctacggaca gtggctcttg ttcgaagaca
     2221 tccagcccgg cggcgtgaat ttcgcctttc tgcaatgcag caatcagtgc attttcatca
     2281 accaccgggc cacgtccggc attaatgaaa atggcggagg atttcatttt ggcgaattgt
     2341 tctgcgccaa acagatgatg cgtctcatca gttaacggca ggatcaggca aacgaaatct
     2401 gactcttgta acagagtatc caaatcgcag tagcgggcgt tgaagcgttc ttctgcttct
     2461 ttatggtggc ggcgcgcgtt atagaggatg ggcatgttga agccaaagtg cgcacgttgt
     2521 gccagcgcca taccgatccg tcccatcccg acaatgccca gtgttttatg gtgaacgtca
     2581 gtgccgtacc agtccgggcc tatgctcgcg gtccattcgc ctgcttttac ccgttctgct
     2641 acctccacaa cccgacgagc ggtagacaac accagcgcca tcagcgtatc ggcgacggtt
     2701 tctgttaata cggttggcgt gtgcatcagc agaatttttc gggcggtaag cgcatcgaca
     2761 tcaaaattgt catagccgac ggagatcgtt gatgtggcac gcagtttcgg cattttttcc
     2821 agcaatgcgg catcaacatt ctcgtttgaa cccagtaaac cttcagcttc ggcaaaaatt
     2881 gctgcatttt gttcgacggt ttgtgggctg aggtttgcca cctggtgaac ggtgaaatgc
     2941 tcttgcaggc gttgcagtaa atcatcaggt aaggctttgt agaggataac ggacggcttc
     3001 atgcttctct ccattcactg ataaggcgat caccttagcc tgaaatatga tgaaggaaaa
     3061 ctgaagaatg ccaggtgatt tctgcatcac ctggcatgaa agggattaca gcgggcttaa
     3121 ggtaatttct acacggcggt tttgcgcctt accttctgcg gtgctgttgc tggcgattgg
     3181 gttagccggg ccaaggccct gagtacggat gcggctggcg tccacgccct gggtgatcaa
     3241 cgcgctggca acggaatccg cacgttgctg ggagagacgc atgttcaggt cgtgaccacc
     3301 cgtgctgtcg gtataaccaa tcacgttaac cgccgttttc ggatactctt tcagcaccat
     3361 tgccacgccg gtcagggtgt tagcgcccgc aggtttcagg gtcgcgctgc tgctgtcgaa
     3421 ggtcacattg ttcggcatat tgaggataat gttatccccg ctgcgggtta cgctaacacc
     3481 agtgccgcgc attttgtcac gcagcttcgc ttcctgcaca tccatgtaat aacccacgcc
     3541 gccgcccaga gctgcgcctg ctgctgcgcc aatcagcgcg cctttaccgc gatctttctt
     3601 cgaagaagag agcgcaccaa tacccgcgcc cacgagagag cccagacctg cgccgatagc
     3661 agatttacct gcttcgcgtt cgccggtgta agggttagtt gtgcagccag ataccgccag
     3721 agcgccactc actacggcgg caataagata aacacgtttc ttcattgtta atccttaata
     3781 acctttttta ttctttgcca cgggttccgt ggcgggagat tatgccgcgt gaacatgaag
     3841 attattcctg ggaatactcg gaaatttgta agtaatattt aactgctcaa tacatctaac
     3901 ctttcaggag tcttcggttt ggccaactca tcctcacgat attccgttct gactgccgcc
     3961 cactgggggc ccatgctggt tgaaaccgac ggcgaaaccg tgtttagctc gcgtggcgcg
     4021 ttagccacag gaatggaaaa ctccttgcag agcgcggttc gcgaccaggt tcacagcaat
     4081 acgcgggtac gatttccaat ggtgcgcaaa ggctttctcg cgtcaccgga aaacccgcaa
     4141 ggcattcgtg ggcaggatga atttgttcgc gtgagttggg atgaggcgct ggatcttatt
     4201 caccaacaac ataaacgcat tcgtgaggct tatggtccgg catcgatttt tgctggttcc
     4261 tacggctggc gttcaaacgg cgtgctgcat aaggcctcga cattattaca acgctatatg
     4321 gcgctggcag gcggttatac cgggcatctg ggggattatt cgactggtgc ggcacaggcg
     4381 atcatgccgt atgtcgtagg tggcagcgaa gtttatcaac agcaaaccag ttggccgctg
     4441 gtgctggaac atagcgatgt cgtggtgctg tggagtgcta atccactcaa tacgctgaaa
     4501 attgcgtgga atgcatccga tgagcagggg ctttcttact tttctgcact gcgtgacagt
     4561 gggaaaaagc tgatctgcat tgatccaatg cgatcggaaa ccgtcgattt ctttggcgat
     4621 aaaatggagt gggtggcacc gcacatgggc accgatgttg cgctgatgct ggggatcgcc
     4681 catacgctgg tggaaaatgg ttggcacgac gaagcgtttc tggcgcgttg caccacaggt
     4741 tatgccgtct tcgcctctta tttgctgggc gagagtgacg gaatagcaaa aaccgccgaa
     4801 tgggcagcag agatttgtgg tgttggcgca gcgaaaatcc gcgagctggc ggctattttc
     4861 caccaaaata ccaccatgct gatggcaggc tggggaatgc agcgccaaca gtttggtgag
     4921 caaaaacact ggatgatcgt cacgctggca gcaatgttgg ggcaaatcgg cacacccggc
     4981 ggcggttttg gtctttctta ccattttgcc aatggtggta accccacgcg gcgttctgcg
     5041 gtgctctctt ccatgcaggg cagcttgccg ggtggctgcg atgcggtgga taaaatccct
     5101 gttgcccgca ttgttgaagc actggaaaac cctggtggcg catatcaaca caacggtatg
     5161 aaccgacatt tcccggatat tcgttttatc tggtgggcgg gcggtgccaa ctttactcat
     5221 catcaggata ccaatcgtct gatccgtgcc tggcaaaaac cggagctggt ggtgatctct
     5281 gaatgcttct ggacggcggc ggcgaaacac gcggatatcg ttctgcctgc gactacctct
     5341 tttgagcgta atgatctcac catgaccggt gattacagta atcagcatct ggtgccgatg
     5401 aagcaagtgg tgccgccacg ctatgaagcg cgtaatgatt ttgatgtttt tgccgagttg
     5461 agtgaacgtt gggagaaggg cggctatgcg cggtttacgg aaggaaaaag tcagctgcaa
     5521 tggctggaaa cgttttataa cgttgcccgg cagcgcgggg caagccagca ggttgaattg
     5581 ccgccatttg ctgagttctg gcaagccaac cagttaattg agatgccgga aaacccggac
     5641 agcgagcggt ttattcgctt cgctgatttt tgccgcgatc cgctggcgca tccgttaaaa
     5701 accgccagcg gcaagattga aatcttctca cagcgtattg ccgattacgg ttacccggat
     5761 tgccctgggc atccaatgtg gctggagccg gacgaatggc agggcaatgc cgaaccagaa
     5821 cagttgcagg tactttctgc ccatccggcg caccgcctgc acagccagct gaattacagt
     5881 tctctgcgcg aattgtacgc ggtagcaaac cgtgagcctg tcaccattca tcctgacgat
     5941 gctcaggcgc gcggcataac cgaaggggat atggtgcggg tgtggaactc ccgtgggcag
     6001 atccttgccg gagcggtcat tagcgaggga attaaacctg gcgtgatttg cattcacgaa
     6061 ggggcatggc cggatctgga tttaaccgct gacggtattt gtaaaaacgg cgcagtgaac
     6121 gtgctgacca aagatctccc cagctcgcgg ctggggaatg gctgtgcggg taatacggcg
     6181 ctggcatggc tggaaaaata caacggtccg gaactgacac ttacagcgtt tgaaccaccg
     6241 gccagctcat aatccaggtg ggtagttggg tttcatcctg ccatgcgcaa tcgacaatgt
     6301 gaaaaccctg cgcacggtaa aaatctatcg ccggttgatt tttttgataa acctccagca
     6361 tcagatgggg atagcgctgc tgcacatact gcatcagcgc cttaccaata ccgcgtctga
     6421 cggccttcgg tgcgacaaac attgctgcca gaaatcggcc ttccataatg ctgacaaaac
     6481 cgagaagctt accgtcctct tcccagaccc agttttgcgc gttggcaaga taggcatccc
     6541 gcaccagcgg aatgcagtca cgccagtaat tcgcttttat aaagggatgc ccccaggttg
     6601 tactttccag ccacagttcg aggatcgcgg ggagttctga acgttgcgct tcccgaatca
     6661 tggtttattt cccggatagc aacagcagcc aaccacatga tcattcacca gcccacatgc
     6721 ctgcataaag gagtaacaga ttgtggtgcc gacaaactta aaaccacgtt ttttcagtgc
     6781 cttagatagg gcgtcggagg cggacgtaga tgtgggaatt tcgctcaacg ttgtggcttg
     6841 tgtcacctgt ggctgatgat ttacaaacga ccagacaaag tcggcaaacg gttcgccgtt
     6901 ctgttccatt tgcaggtacg cccgcgcatt accaataatt gcctgaattt tccctcgatg
     6961 gcggataatc ccggcgtcct gtaccagtct ttcgacatcc tcttcctgca ttgctgcgac
     7021 cttcaccgga tcgaactgat gaaagcaggc gcgatagttt tcgcgttttt tgaggacggt
     7081 gatccacgat aacccagcct gctgcccttc aaggcagatc atttcgaaca gttttttact
     7141 gtcagtttca ggcacgcccc attcattatc atggtaggca atataaagcg ggtcctgact
     7201 cacccagccg caacgttcca tactttccct cgctatgtac ccgatcgtaa aaatttcagc
     7261 cgcttttctt gacgcatcgg tgaaaaagtc aatatttaat acagggctac tatgcccgat
     7321 atccttcatg caatgacaat aatattgccg aattcggctc ttccctggag tcgtgttaat
     7381 gatcataaaa aaaagcggtg gtcgctggca gctaagcctg ctggcgagcg tggtaatcag
     7441 tgcctttttt ctcaacacag cttacgcctg gcaacaagaa tatatcgttg atacgcaacc
     7501 cggacattcc acagagcgtt acacctggga tagtgatcat caacctgatt acaacgatat
     7561 tttgtcgcaa cgtattcaaa gtagccaaag ggcgctggga ctggaagtca atctggcgga
     7621 agaaactcct gtggatgtga ccagcagtat gagtatgggc tggaattttc ctttatatga
     7681 acaggttaca accggcccgg tcgcggcatt acattacgat ggcacaacca cctcgatgta
     7741 taacgagttt ggcgacagta ctaccacgct gaccgatccg ttatggcatg ccagcgtgag
     7801 tagcttaggc tggcgtgttg actcccggct tggcgatctc cgaccctggg cgcaaatcag
     7861 ctataaccag caatttggcg agaatatctg gaaggcgcaa tcaggcctga gccggatgac
     7921 ggcgacaaac cagaacggca actggctgga tgtcaccgta ggcgctgata tgttgctcaa
     7981 tcaaaatatt gccgcctatg ccgcgctaac tcaggcagaa aataccacta ataatagcga
     8041 ctatctgtat acgatggggg ttagcgccag attttaacgt aacagtcaca attgaaacca
     8101 ttgaataaca acagttgtgt cgatagtggg tgctaactta ccaaataata aatttggtga
     8161 ataattgtcg cgtcattcat tcctgaacta aggcatttca ttccgttctg atggcatttc
     8221 atgccgtttt tccccaggca taaaatgcac ttcgttatgg ttgtcggcag agatttttcc
     8281 tttttattac tgcaggaata ctgccatgac accttcaaat tatcagcgta cccgctggct
     8341 gacactcatc ggtactatca ttacccagtt tgcactgggg tcggtttata cctggagcct
     8401 gtttaatggc gcgctttctg ccaagctgga tgcgccggta agccaggtcg ctttctcttt
     8461 cggcttgtta agtctggggc tggcaatttc gtcttctgtt gcgggcaaat tgcaggaacg
     8521 ttttggcgtt aaacgcgtca ccatggcttc cggcattttg ctgggattag gcttcttcct
     8581 gaccgcgcat tccaacaacc tgatgatgct gtggttaagc gccggtgtgc tggtcggtct
     8641 ggcggatggc gcgggttacc tgctgacgct ctctaactgc gtgaagtggt tcccggagcg
     8701 taaaggtctg atatccgcgt tcgctatcgg ttcttatggt ctgggtagcc tgggtttcaa
     8761 atttatcgac acgcagctgc tggaaacggt gggtctggaa aaaacctttg tgatttgggg
     8821 agcgattgcg ctggtgatga ttgttttcgg cgcaacgtta atgaaagacg caccaaaaca
     8881 ggaagtgaaa accagcaatg gtgtggtgga gaaagattac acgctggcag agtcgatgcg
     8941 taaaccgcag tactggatgt tagcggtaat gttcctgact gcctgcatga gcggcctgta
     9001 cgtgattggg gtagcgaaag atatcgccca aagtctggca caccttgatg tggtttccgc
     9061 agccaatgca gtaacggtta tttccatcgc taacctttca ggtcgtctgg tgctgggcat
     9121 tctgtctgac aaaattgccc gtatccgtgt tatcaccatt ggtcaggtga tttcgctggt
     9181 gggtatggcg gccctgctgt ttgcaccatt aaatgcagtg acgttctttg cagcgattgc
     9241 ctgcgtggca tttaactttg gcggcactat taccgtcttt ccatcactgg tcagtgagtt
     9301 cttcggcctc aataacctgg cgaaaaacta cggtgtgatt tacctcggtt tcggtatcgg
     9361 tagcattttt ggttcgatta tcgcctcact gtttggcggt ttctatgtga ccttctacgt
     9421 gattttcgcc ctgctgattc tgtcattggc gctttctacg acgattcgtc agccagagca
     9481 gaaaatgttg cgtgaggcgc atggctccct ttaatgctca gccgcgagct actgggcttc
     9541 agcttccgtg tgcatcaaac gcatcaacga aatgtataaa caatgactcg taaaatttgg
     9601 gccagttaac tggccctttt tactgctttt tgtaacgcta aagtctcttt tcaaacttgc
     9661 atttttgtaa atttgtgctt catgcacact ctttccccac actttttcgc tttgctgtgg
     9721 tctacttatt cgcgcgtgta gattttactt atctgactac ctccgcactt ttcccctgcc
     9781 gggcctgaaa agccgctaag cagggtgtta tcacctgttt gtccagggtt tgtttgcatg
     9841 agatacatca aatcgattac acagcagaag ctgagctttt tgcttgcaat ctatattggc
     9901 ctttttatga atggcgcggt tttttatcgc cgcttcggca gctatgcgca cgattttacc
     9961 gtctggaaag gcgtttctgc tgttgttgaa ctggccgcca ccgtactggt gaccttcttt
    10021 ttactacgtc ttctatcgct gtttggccgc cgcagctggc gtattctggc gtcgctggtg
    10081 gtgctctttt ccgcaggtgc cagctattac atgaccttcc ttaatgtggt cattggttat
    10141 ggcatcatcg cttccgtcat gaccaccgat atcgacctgt caaaagaagt tgttggtctg
    10201 aactttattc tctggttaat cgccgttagt gcattgcctc ttatccttat ctggaataac
    10261 cgctgtcgct acaccttgct ccgacaactg cgaaccccag ggcagcgtat tcgcagcctg
    10321 gcggtcgtcg tactggcggg tattatggtt tgggcaccga ttcgtttgct ggatatccag
    10381 cagaagaaag tggagagggc gaccggcgtt gatttgccga gttatggcgg tgtcgtagcg
    10441 aactcttatc tgccatcaaa ctggctttct gcgttggggc tgtatgcctg ggcgcgggtc
    10501 gatgaatctt ccgataataa ttcattgctt aatccggcga agaaattcac ttatcaggca
    10561 ccgcaaaacg ttgatgacac ttatgtcgtg tttatcatcg gtgaaaccac gcgttgggac
    10621 catatgggca ttttcggcta tgagcgtaat accacgccga aactggccca ggagaaaaat
    10681 ctggcggcgt tccgtggtta ctcctgtgat accgcaacca aactctcact gcgttgcatg
    10741 tttgtacgtc aggggggcgc ggaagataat ccgcagcgca cattaaaaga acagaacatt
    10801 ttcgcggttc tgaagcagtt aggattcagt tctgacctct acgctatgca gagcgaaatg
    10861 tggttctaca gcaacacgat ggcggacaac attgcttatc gtgagcagat tggtgcggag
    10921 ccacgtaatc gtggcaagcc ggtagatgat atgttgctgg tagacgaaat gcagcaatcg
    10981 ctagggcgca acccggatgg taagcatctg atcattctgc ataccaaagg ttcgcatttt
    11041 aactacaccc agcgttatcc gcgtagcttc gcgcagtgga agccggaatg tattggtgtt
    11101 gatagcggct gtaccaaagc gcagatgatc aactcctatg acaactcggt gacctatgtg
    11161 gatcacttta tctccagcgt gattgatcag gttcgcgata agaaagcgat tgtgttctac
    11221 gcagctgacc acggtgagtc aattaatgaa cgcgagcacc tgcacggcac gccgcgtgaa
    11281 ctggcaccgc cggagcagtt ccgcgtaccg atgatggtct ggatgtcaga taaatatctg
    11341 gaaaatccgg ccaatgctca ggcatttgcg cagctgaaaa aagaagccga catgaaagtg
    11401 ccacgccgtc atgtagagct gtacgacacc atcatgggtt gtcttggcta tacttcaccg
    11461 gatggtggaa ttaacgaaaa caacaactgg tgtcacatcc cgcagacaaa agtggcagcg
    11521 gctaactaag cggcctgctg actttctcgc cgatcaaaag gcattttgct attaagggat
    11581 tgacgagggc gtatctgcgc agtaagatgc gccccgcatt cggtgattgg cgcagcctgg
    11641 tagcgcactt cgttcgggac gaaggggtcg gaggttcgaa tcctctatca ccgaccaaat
    11701 tcgaaaagcc tgctcagcga gcaggctttt ttgcatttga agcccatgag gatgagaacc
    11761 tccgggggcg gggaggttcg actcgagcga agcgagagaa cgttgcgcca gcaacggccc
    11821 gcagggcgag ccacgaagtg gcgagtaatc ctctatcacc gaccaaattc gaaaagcctg
    11881 ctcagcgatc aggctttttt tgcatttgta gcccatgagg atgagaacct ccgggggcag
    11941 ggaggttcga ctcgacgtag gcctgataag acgcgccagc gtcgcatcag gcaacggcac
    12001 cgcacttatt gtcggatgcg gcgcgaccgc cttattcgac caacacacca cacattttcc
    12061 tgccaacttt gcgccccata tctcactatc cattaacgga tttgtgacag aaaccattgt
    12121 ctttttttat atttgcttaa atcttttttt gcgttactta tggctggcgc tggcattttc
    12181 cgctgtgcgt tttagcgttc atggtattaa tcgctcagat atccaccttt tcctcagatt
    12241 tttttcatta aatgcataaa acttaatcac caaatgttgc taaatattaa gctgattgtt
    12301 ctggtcatgt tggagtagca caaatttctc tgctgcaaat agctgtttga agttttttta
    12361 atctttgttt gtggattctc accgttggtg taaatcccgg ttggctgtat tgacgttttc
    12421 acattctgtt gacagattgt aggtcacgag gggcatttta tggaggatcc gcactgttac
    12481 actgatgtta attagtacgg catccccacc tcataacgtt gacccgaccg ggcaaaaaac
    12541 aaaaaaggtc aggcagcgac aacccactgc aaagggttaa aacaacaaac atcacaattg
    12601 gagcagaata atgcgtattt ccttgaaaaa gtcagggatg ctgaagcttg gtctcagcct
    12661 ggtggctatg accgtcgcag caagtgttca ggctaaaact ctggtttatt gctcagaagg
    12721 atctccggaa gggtttaacc cgcagctgtt tacctccggc accacctatg acgcctcttc
    12781 cgtaccgctt tataaccgcc tggttgaatt taaaatcggc accactgaag tgatcccggg
    12841 cctcgctgaa aagtgggaag tcagcgaaga cggtaaaacc tataccttcc atctgcgtaa
    12901 aggtgtgaag tggcatgaca ataaagaatt caaaccgacg cgcgaactga acgccgatga
    12961 cgtggtgttc tcgttcgatc gtcagaaaaa cgcgcaaaac ccgtaccata aagtttctgg
    13021 cggtagctac gaatacttcg aaggcatggg cttgccggag ctgatcagcg aagtgaaaaa
    13081 ggtggacgac aacacagttc agttcgtgct gactcgcccg gaagcgccgt tcctcgctga
    13141 tctggcaatg gacttcgcct ctattctgtc aaaagaatat gctgatgcga tgatgaaagc
    13201 cggtacaccg gaaaaactgg acctcaaccc aatcggaacc ggtccattcc agttacagca
    13261 gtaccaaaaa gattcccgta tccgctacaa agcgtttgat ggctactggg gcaccaaacc
    13321 gcagatcgat acgctggtct tctctattac ccctgatgct tccgtgcgtt acgcgaaatt
    13381 gcagaagaat gaatgccagg tgatgccgta cccgaacccg gcagatatcg ctcgcatgaa
    13441 gcaggataaa tccatcaacc tgatggaaat gccggggctg aacgtcggct acctctcgta
    13501 taacgtgcag aaaaaaccgc tggatgacgt gaaagttcgc caggcactga cctacgcggt
    13561 gaacaaagac gcgatcatca aagcggttta tcagggcgcg ggtgtatcag cgaaaaacct
    13621 gatcccgcca accatgtggg gctataacga cgacgttcag gattacacct acgatcctga
    13681 aaaagcgaaa gccttgctga aagaagcggg tctggaaaaa ggtttctcca tcgacctgtg
    13741 ggcgatgccg gtacaacgtc cgtataaccc gaacgctcgc cgcatggcgg agatgattca
    13801 ggcagactgg gcgaaagtgg gcgtgcaggc caaaatcgtc acctacgaat ggggtgagta
    13861 cctcaagcgt gcgaaagatg gcgaacatca gacggtaatg atgggctgga ctggcgataa
    13921 cggggacccg gataacttct tcgccaccct gttcagctgc gccgcctctg aacaaggttc
    13981 taactactca aaatggtgct acaaaccgtt tgaagatctg attcaaccgg cgcgtgctac
    14041 cgacgaccat aacaaacgcg ttgaactgta caagcaagcg caggtggtga tgcacgatca
    14101 ggctccggca ctgatcatcg ctcactccac cgtgtttgaa ccggtacgca aagaagtcaa
    14161 aggctatgtg gttgatccat taggcaaaca tcacttcgaa aacgtctcta tcgaataatt
    14221 aaaagccata caagactgat ggcaaaggca aaactgcctg atgtgctccg cttatcaggc
    14281 ctacgaaaat tctgcaatgt attgaatttg cacgattttg taggccggat aaggcgttaa
    14341 cgccgcatcc ggcataaaca aagcgcactt tgtcaacaat ctgtataccc ggtggcgctg
    14401 tgcctctgcg tgcagcgcca tccggcagca atacttcatt ctctgtccga caagggcggg
    14461 aattgatttg tgagcaatac agacacgcag ttccaggctg cgggtcacta tagagaatcc
    14521 gggttatgtt gcagtttatt ctccgacgtt tgggactcgt catccccacg tttatcggta
    14581 ttacccttct cacatttgcc tttgtccaca tgatcccggg cgatccggtg atgatcatgg
    14641 caggcgaacg tgggatctcc cctgagcgtc acgcgcagct gctggctgaa ctcggcttag
    14701 ataaaccgat gtggcagcag tatctccatt acatttgggg cgtaatgcac ggcgatctag
    14761 gcatttcaat gaaaagccga attccagtat gggaagagtt cgtgccgcgc tttcaggcca
    14821 cgctggaact tggcgtctgc gcgatgattt ttgctacggc agtcggtatt ccggttggtg
    14881 tgctggctgc ggttaaacgc ggttccattt tcgatcacac agcggttggc ctggcgctga
    14941 ccggctactc gatgccgatc ttctggtggg gcatgatgct gatcatgctg gtttcggtgc
    15001 actggaacct gacgcccgtc tccggtcgcg tgagcgatat ggtgttcctc gatgactcca
    15061 atccgttaac cggttttatg ctaatcgaca ccgccatctg gggtgaagac ggcaacttta
    15121 tcgatgccgt cgcccatatg atcttgcctg ccattgtgct gggtactatt ccgctggcgg
    15181 tcattgtgcg tatgacacgc tcctcgatgc tggaagtgct gggcgaggat tacatccgca
    15241 ccgcgcgcgc caaagggcta acccgcatgc gggtgattat cgtccatgcg ctgcgtaacg
    15301 cgatgctgcc ggtggtgacc gttatcggcc tgcaggtggg aacattgctg gcgggggcga
    15361 ttctgaccga aaccatcttc tcgtggcccg gtctgggacg ctggttgatt gacgcactgc
    15421 aacgccgcga ctatccggta gtgcagggcg gcgtattgct ggtggcgacg atgattatcc
    15481 tcgtcaactt gctggtcgat ctgctgtacg gcgtggtgaa cccgcgtatt cgtcataaga
    15541 agtaagggga catcatgtca caggttactg aaaataaagt gattagcgca ccggtgccga
    15601 tgaccccgtt acaggagttc tggcactatt ttaaacgcaa caaaggcgcg gtcgtcgggc
    15661 tggtttacgt cgtcatcgtg ctgttcatcg cgatctttgc caactggatt gcaccctata
    15721 acccggcgga acagttccgc gatgcactgc tcgccccgcc agcctggcag gaaggcggca
    15781 gcatggcgca cttgctgggc accgatgacg taggccgtga tgtgctgtcg cgcctgatgt
    15841 acggtgcgcg cctgtcgctg ctggttggct gtctggtagt tgtgttatcg ctgattatgg
    15901 gcgttattct cggcctgatc gccggttact ttggcggcct ggtcgataac atcattatgc
    15961 gcgtggtcga tatcatgctg gcgctgccaa gtctgctgct ggcgctggtg ctggtggcaa
    16021 ttttcggccc gtcgattggt aacgccgcgc tggcactgac cttcgttgcc ttgccgcact
    16081 atgtgcgctt aacccgcgcc gccgtgctgg tggaagttaa ccgcgattac gtcaccgcgt
    16141 ctcgcgtggc gggtgccggg gcgatgcgtc agatgtttat taacatcttc ccgaactgcc
    16201 ttgcgccgct gattgttcag gcgtcgctcg gtttctctaa cgccattctc gatatggctg
    16261 ctcttggttt cctcggcatg ggggcacagc cgccaacgcc tgagtggggc accatgctct
    16321 ccgacgtgtt gcagttcgcg caaagcgcct ggtgggtcgt gaccttcccg ggtctggcga
    16381 tcctgctgac ggtgctggca tttaacctga tgggtgacgg tctgcgtgac gcgctcgatc
    16441 ccaaactgaa gcagtaagag gttcgagatg gcgttattaa atgtagataa attatcggtg
    16501 catttcggcg acgaaagcgc gccgttccgc gccgtagacc gcatcagcta cagcgtaaaa
    16561 cagggcgaag tggtcgggat tgtgggtgag tccggctccg gtaagtcggt cagttcactg
    16621 gcgattatgg ggctgattga ttatccgggc cgcgtaatgg cagaaaaact ggagtttaac
    16681 ggccaggatt tgcagcgtat ctcagaaaaa gagcgccgca acctggtggg tgccgaagtg
    16741 gcgatgatct tccaggaccc gatgaccagc cttaacccgt gctacaccgt gggtttccag
    16801 atcatggaag cgattaaggt gcatcagggc ggtaataaga gcactcgccg tcagcgggcg
    16861 attgacctgc tgaatcaggt cggtattccc gatccggcat cgcgtctgga tgtttacccg
    16921 catcagcttt ccggcggcat gagccagcgc gtgatgatcg ccatggcgat tgcctgtcgg
    16981 ccaaaactgc tgattgccga tgaaccgacc accgcgctgg acgtgaccat tcaggcgcaa
    17041 atcatcgaac tactgctgga gctacagcag aaagagaaca tggcgctggt gttaattacc
    17101 catgacctgg cgctggtggc ggaagcggca cataaaatca tcgtgatgta tgcaggccag
    17161 gtggtggaaa ccggtgatgc gcacgccatc ttccatgcgc cgcgtcaccc gtatactcag
    17221 gcattgctgc gtgcgctgcc agaatttgct caggacaaag aacgtctggc gtcgttgcca
    17281 ggtgtcgttc ccggtaagta cgaccgcccg aacggctgcc tgcttaaccc gcgctgcccc
    17341 tatgccactg acagatgtcg cgctgaagaa ccggcgctga atatgctcgc tgatgggcgt
    17401 cagtccaaat gccattaccc acttgatgat gccgggaggc cgacactatg agtacgcaag
    17461 aggccacctc gcaacaaccg ctgttgcagg ctatcgacct gaaaaaacat tatccggtga
    17521 agaaaggcat gttcgcgccg gaacgtctgg tgaaggcgct gggcggcgtt tcgtttaacc
    17581 ttgaacgtgg caaaacgctg gcagtagtgg gcgaatctgg ctgcggtaaa tcgaccctcg
    17641 gtcggttgct gacgatgatc gaaacgccca ccggtggtga gctgtattac caggggcagg
    17701 atctgctcaa gcacgatccg caggcgcaga agctgcgtcg gcagaaaatc cagattgtct
    17761 tccagaaccc ttacggttcg ctgaatccgc gtaaaaaagt tgggcaaatt ctcgaagagc
    17821 cgctgcttat taacaccagc ttcagcaaag aacagcgtcg ggaaaaagcc ctgtcgatga
    17881 tggcgaaagt cggcctgaaa accgagcact atgaccgcta tccgcatatg ttctccggcg
    17941 gacaacgtca gcgtatcgct attgcccgtg gtctgatgct cgacccggac gtggtgattg
    18001 ccgatgaacc ggtttctgcg ctggacgtct cagtgcgtgc gcaggtgctg aatctgatga
    18061 tggatttgca gcaggagttg gggctttctt atgtctttat ctcccacgac ctgtcggttg
    18121 tggagcacat tgctgatgaa gtgatggtga tgtacctggg ccgctgcgtg gagaaaggaa
    18181 cgaaggacca aatcttcaat aacccgcgcc atccgtacac ccaggcgctg ctctccgcga
    18241 cgccacgcct gaacccggac gatcgtcgtg aacgcatcaa actcaccggt gaactgccaa
    18301 gcccgctgaa tcctccgccg ggttgcgcct tcaacgcccg ctgtcgtcgg cgcttcggcc
    18361 cctgcaccca gttgcagccg cagctaaaag actacggcgg tcaactggta gcttgttttg
    18421 ctgttgatca ggatgaaaat ccgcagcgtt aactcacaaa accggggcca tgcgctccgg
    18481 tttactcaat cagcttcaac agtggcgaca agcatagcaa aatgccgtaa agcagaatta
    18541 gccaggcttt aaatccgcga agtttttcca gttgtgcgac tttatagatc aggaaaaacg
    18601 gaatgaggca cgacacaatg ccatataacg ggcttcccaa ctgaaagaac accagcaccg
    18661 atacacgaaa cgaaacccaa atcgtcaatg taatgacgat aaaagcgcag atcgccagag
    18721 tcagcatgcg tgagttaatt ttcttggtat caataattcg gcttaacaga ttgagaataa
    18781 tgcctttaat agcctcgtgg aaaccgagat aaatgccaaa gaatgcggtc agtacggcaa
    18841 agatattcag caccgtagag gtgatatgaa tgatatgtcc agggatcacc tgcgcggcca
    18901 gcgccagtgc cgagatattt tgttcaaagg cagaaacggc ttcttcgtgg ctaattgaga
    18961 aggtaaacga aaaggcaaaa aacaggatca ccgcgatgag cgtgatataa ctaattcggt
    19021 gggtacgcag cgcgagccgg gttgccagta ctttatctgc ttcccgttta cgataggcaa
    19081 tattcattgg gttaagtacc tgaataaata ccgcagaaaa gaagcaaaat ggaattgtaa
    19141 gcaggacatc gcggaaaaag acggaggctt gcgggaaggc ggttatattg gcgaaattcc
    19201 agtgcgggat catcgcaaaa ccgaacacga caataatccc tactttgacc accaccattg
    19261 gcccggaaat cttaaacagt aatcgttcac caccagacgc aatcgccacc agtacggcga
    19321 aaatagcgac tttataaagt agagattgtg aaagatcggc atcggttaaa ccgaaggttt
    19381 tcaggtacga ggcgctgtcg aaaaccacgg agagagagta gataaaaatc ccgtggataa
    19441 tcatcaaaaa gtagataacc ccgaggaaaa ttccccagtt cttccccagg taatgactga
    19501 taatatcggt gtagtcatta caggaatcgc tttcagaaag ggtttttaaa taaatgtcct
    19561 gcactaccca ggtggcagga taagcaatga tcgctgcggt aataaatacc caaattccct
    19621 tcaagccaat ttgtactggc atcagcacgg ttccggcacc aatcgccatg cctatgcata
    19681 ataaaaccca gccgaagtcg tagcgtgtaa acggcaattt ctgattgtgt ttcgatagtg
    19741 tgttgtgctg cataaagtcc tgtccagaaa gaagtcggcc tcgcaaaaat gaataaaccc
    19801 agtttctctt gcgcaacagg tggagaagtc gaatacgcag tatgtaaaaa atgaggcaac
    19861 agattcactg gggaggtcgc gaggaggcgc aattctacaa aattgcgcat ttcactacca
    19921 gtctggagtc agcccggata tgaactgcaa ctattgcaaa taaagaaatt aagctggaag
    19981 gcatcgcgca atgaggaaga ttgccgggtg ctgacagtaa aaagcaatcg taaaatcagc
    20041 ggcaagccgg aagggttccg gtgaggcgca atgttgcggg ggctttatcc ctggtggcat
    20101 tggttgctgg agagagaaaa cccccgcacg ttgcaggtat atacctgaca acataacggg
    20161 ggctaatctt gaacctagac cactcaagaa tagccgcgaa acgttgtcat tacaacacag
    20221 gcggctatat gacgctcgca gagctgggca tggccttctg gcatgattta gcggctccgg
    20281 tcattgctgg cattcttgcc agtatgatcg tgaactggct gaacaagcgg aagtagcgtg
    20341 tcatgtgggc gtcaggctgc cgtaatggca atttgcgccc ggatcaggcc gcagaggtta
    20401 aactctgcgg cctttttcgt gcttactgcg ggtaaggcac ccagtcgccg ccgttcaggc
    20461 gaacgtacgg tttatcctgg tattgaataa ctactgcatt tgagttctcg gagaccggtg
    20521 ctgtttgtgg caacccactg gtgagttttt tccagtcaac attgtcttcg gtgaaaatct
    20581 tgccatcaag aacgcgaacc accagatcgg agatagccag gaagctgctc ggttggtcaa
    20641 tgacaatcgg tgccccctga tgtggtgcct tcatgccgaa gaatttcacc ccaacgggga
    20701 cgtctgtgat agacgggcta gggatatcac gtaggccaga tacctgcatt ctgtcgccct
    20761 tcagcgcgcc gccgtgttcc ggcaccacga ccaccatcac tttacgaccc gatttctcca
    20821 gttcagtaaa gaaggcgtcc agttcatcaa agaatttctg cgcccgcgct ttgtaatccg
    20881 ctgttttgct gacccccgga taatggttgc cgtcatgcag tggaagcgtg ttgtagaacg
    20941 tggcactacg gctgttttta tctttttcgg taacgtccag ccagcggtta agcacggcgg
    21001 tatcgtcata aaccggcgaa ccatcaaagc ccagcaaaat aaccggcaga tttgtttgat
    21061 ccatcaattc agtctgcatg ccgccatttt cgcgaacttc tttcaaaaaa ccgccgaact
    21121 ggccgttatg ccccatcatc aggtgctggg taaagcccag tttcgaaagg ttatcaaaca
    21181 gatagcagtc gttatttgcc ggttgataca gattagtgtg cgaagtctgc ccgcagctgg
    21241 cgcgcagtaa acggatcgcc gccgggccac tgtaggaggt ggcggagtta aagttcttga
    21301 actcaatatc gaaatgcgac cacagtggat gcgacatcaa cccggcggct tctatatccg
    21361 accaggaaag cgaacagatg ttaatcacca gtagttcaaa tggctgagca tcagcgggca
    21421 gcgaagacgg gaaggtcgat ttacgttttg cctccgcgtt atagaaatta ttcagccagg
    21481 cgttaaggtt cgccgttgtt ggcggtgcag tttgtgcggg catatcaccc actaccggtg
    21541 cgccacccgt cgccgcaacg gttgccgctg cgttaccacc cgtcgttgtt acagtggtcg
    21601 tcggttgtcc ggctggccac aaggagaaac ttggtcccgc cagggtaagt acgttcagcc
    21661 atagcagtat ggcaaccaca aaaacggtaa tgcgaatcca ttgtgacagg aataaccagg
    21721 ccactaataa aacaaaaatg gccccaatca tctgccagtt aataaagcgt gtgacaaggt
    21781 cgattaaata atcggtactg aaccccgcca cctgcgaacc ctggctcatt atgctttccg
    21841 ggccaggcaa ccaggtgtca tgccagaaca aagcaaagcc gatcggcagg gcaatccagt
    21901 ggcgcaagcg atgcaggctg tagcgcggaa tgggcatcag cagaaacgcg gcaaacacca
    21961 aattgaggag cggatggaag ttaagatatc ccgcccacaa caggccgaac ttaaccagaa
    22021 aatagaagtt ccagccggaa aggccgcgcc agtattgcca gagggaagaa ggcatggcgg
    22081 tattttgcgt aaattgagtc attttttggt tgccctggct ttttccgtgc ggcgtaacgt
    22141 cccggccggt ttaacataac gaggtttagc aaagaacaaa cgtaaggtgt cgcgaatgcg
    22201 tcgcaaagag tgccgcgcca gatagcccag cgggaaaaat atcagtgcgc aaacgacaat
    22261 aatttcaatg atatcgctga tggtcatcat gatgagcgct ccacagcatc atctaacagt
    22321 cgcatgggtt ctggtattcg tcgccagtgg cgaccatcgt gctcggcatt gatgaccggt
    22381 ttagaacttt gcgttaaagg cagcggcatg ccccattgtt ctggggcaag caagcgcatc
    22441 tgcaccagct cggcactgat ttgatcatct tcaaaccaga ccatacggtt tgagaaaatg
    22501 tcgccagtag gcaatgggaa aatatgattc aacgcggtat ccagatcgtt aatccgacag
    22561 aatgagagaa acagcaccag ccgattaccg ccaatggtca tgatatcgcc ggtacggtta
    22621 gggcgacaca gcgtcagggc ttgttcaacg cggatacccg gtaccggacg tagggcaacc
    22681 agaacgcctt taccgtgggc aggtaataga gggttattca tcatgttgtt gacggcatta
    22741 cagaacacat cccacttctg gaaaccacgc agtttgagcg gctgggtcat tgacagcaag
    22801 gtagtgatat cttccggcac atagcgacta aacttctgcc cttgcacgct ttcgatcatc
    22861 gtcagacaac gggagagtgg cgcattccac ggaataacca tatttgcacc gcaggccaat
    22921 aacaaacgtt catcggtggc gcgcaggcta gcggtatttt cccgcacgag gattttcatc
    22981 gcactaccgc gctggcgacg cagggtatga atgctgcggg ccagtggctc gatttgcgca
    23041 ttttgctgta aagaaaagac caccgtcgcc gcctgagcgg tacgggcttc attgaacagg
    23101 acttcgttat tgttgaacag ttgccagtgt tccgatagcg gcggcgcacc ttccagtaca
    23161 gcaacattac tcagaatgcg tttttcgtcg ctgcgtggtt ggatctccgc ctcttcgctt
    23221 tgaactaatg tccagatacc attttgttgc tgaacgctaa gctgctgacg ggcgctgacc
    23281 cctttttcgt tgcaccagaa ggcaatatcc agcaaatgtt ggtcaccctg aaaacgcaaa
    23341 ctggcaagac caaaaagtga acggtattcc tcaagcaaca atgaaaattg tttatcgtta
    23401 ttatttccgg gattaattac caaaagcgaa caatggttta acctgctcca tttattcatt
    23461 ttatccaacc atgagcgaag ccgctcggca ggaatgtttt gccatgcgtt atttgcgcaa
    23521 acaagaataa aaaggtaatt atggggatca atagaacact gcaaatcacg ggtcaaatag
    23581 tatagacctt tttcatgatt tagcattgaa aataatttta ttttttccgg cccttgagaa
    23641 tcatctaatt gaaagatttt cgccggatcg ctgtccatgc taatgaccgc gacgtgtgcg
    23701 gtttcagcct gggatgcaat tgtttgattc gccagactga tagcatcttc atggcgatcg
    23761 acgttaaacc accagacgcc gcctgctggc atatgtcgca gctcatccca taatgatgag
    23821 ataccgatag agaatacagg gtccacaatg tccctcatgt caccattttg tctgattatc
    23881 agtttactag cgaaagctaa gaaataaacc taccattgaa attaaaactt atcagattta
    23941 gcatgtgaat caatttagaa ggtcacaaga cgtgtgattt tagtcttttc gtttttaact
    24001 ttttctgttg cgtaacggaa agtcaaaaag tgagcaaatt cccgtctcgc cgtaaacaat
    24061 aaaccggcga aatgctcacg gttagaattg tctcatgaac ggtacggtta tttcataggg
    24121 atcaagcaaa atgaataaca atgaaccaga tactctgcct gatcccgcga taggctatat
    24181 cttccagaat gatattgtgg cgttaaagca ggcattttca ctgcctgata ttgattatgc
    24241 cgatatttcc caacgcgaac agttggccgc ggcattaaaa cgctggccgt tgctggcaga
    24301 gtttgcgcaa caaaagtagg ggattggtga atggccgtac tgggattgca gggggtgcgg
    24361 ggaggcgtgg ggacaacaac catcaccgcc gcattagcct ggtcattaca aatgttggga
    24421 gaaaatgttc tggtggtcga tgcctgcccg gacaacttgt tgcgcctgtc atttaatgtt
    24481 gattttaccc accgtcaggg ctgggccaga gcgatgctgg atggccagga ctggcgtgac
    24541 gctgggttgc gctacacctc gcagctcgat ttgctgcctt ttggtcagtt atccattgaa
    24601 gaacaagaaa atccacagca ctggcaaacc cggctgagcg atatttgctc cggcttacag
    24661 caactaaaag ccagcgggcg ttaccagtgg attttaatcg acttaccgcg tgatgcctcg
    24721 cagataaccc accagctgct gagtttgtgc gatcactcgc tggctatcgt caatgtggat
    24781 gccaactgcc atatccgcct gcatcagcaa gcgctgccgg atggcgcaca tattttgatt
    24841 aatgacttcc gtattggcag tcaggttcag gacgatattt accagctttg gttgcaaagc
    24901 cagcgccgat tactgccgat gctcattcat cgtgatgaag cgatggctga atgcctggcg
    24961 gctaagcaac cagtaggtga atatcgcagt gatgcgctgg cggctgaaga gatactgacg
    25021 ctggcgaact ggtgcctgtt gaactactcc gggctgaaaa cgccagtcgg gagtgcatca
    25081 tgagtatcct gacccggtgg ttgcttatcc cgccggtcaa cgcgcggctt atcgggcgtt
    25141 atcgcgatta tcgtcgtcac ggtgcgtcgg ctttcagcgc gacgctcggc tgtttctgga
    25201 tgatcctggc ctggattttt attccgctgg agcacccgcg ctggcagcgt attcgcgcag
    25261 aacataaaaa cctgtatccg catatcaacg cctcgcgtcc gcgtccgctg gacccggtcc
    25321 gttatctcat tcaaacatgc tggttactga tcggtacatc gcgcaaagaa acgccgaaac
    25381 cgcgcaggcg ggcattttca ggtctgcaga atattcgtgg acgttaccat caatggatga
    25441 acgagctgcc tgagcgcgtt agccataaaa cacagcatct tgatgagaaa aaagagctcg
    25501 gtcatttgag tgccggggcg cggcggttga tcctcggtat catcgtcacc ttctcgctga
    25561 ttctggcgtt aatctgcgtt actcaaccgt ttaacccgct ggcgcagttt atcttcctga
    25621 tgctgctgtg gggtgtagcg ctgatcgtac ggcggatgcc ggggcgcttc tcggcgctaa
    25681 tgttgattgt gctgtcgctg accgtttctt gccgttatat ctggtggcgt tacacctcta
    25741 cgctgaactg ggacgatccg gtcagcctgg tgtgcgggct tattctgctc ttcgctgaaa
    25801 cgtacgcgtg gattgtgctg gtgctcggct acttccaggt agtatggccg ctgaatcgtc
    25861 agccggtgcc attgccgaaa gatatgtcgc tgtggccgtc ggtggatatc tttgtcccga
    25921 cttacaacga agatctcaac gtggtgaaaa atactattta cgcctcgctg ggtatcgact
    25981 ggccgaaaga caagctgaac atctggatcc tcgatgacgg cggtagggaa gagtttcgcc
    26041 agtttgcgca aaacgtgggg gtgaaatata tcgcccgtac cactcatgaa catgcgaaag
    26101 cgggcaacat caacaatgcg ctgaaatatg ccaaaggcga gttcgtgtcg attttcgact
    26161 gcgaccacgt accaacgcga tcgttcctgc aaatgaccat gggctggttc ctgaaagaga
    26221 aacagctggc gatgatgcag acgccgcacc acttcttctc gccggacccg tttgaacgca
    26281 acctggggcg ttttcgtaaa acgccgaacg aaggcacgct gttctatggt ctggtgcagg
    26341 atggcaacga tatgtgggac gccactttct tctgcggttc ctgtgcagta attcgtcgca
    26401 aaccattgga tgaaattggc ggtattgctg tcgaaacggt aaccgaagat gcgcacacgt
    26461 ctctgcgcct gcaccgtcgt ggctacacct ctgcatatat gcgtattccg caggcggcgg
    26521 ggctggcgac cgaaagtctg tcggcgcata tcggtcagcg tattcgctgg gcacgcggga
    26581 tggtgcaaat cttccgtctc gataacccgc ttaccggtaa agggctgaaa ttcgcccagc
    26641 ggctgtgcta cgtcaacgcc atgttccact tcttgtcggg cattccacga ctgatcttcc
    26701 tgactgcgcc gctggcgttc ctgctgcttc atgcctacat catctatgcg ccagcgttga
    26761 tgatcgccct gttcgtgctg ccgcatatga tccatgccag cctgaccaac tccaagatcc
    26821 agggcaaata tcgccactct ttctggagtg aaatctacga aacggtgctg gcgtggtata
    26881 tcgcaccacc gacgctggtg gcgctgatta acccgcacaa aggcaaattt aacgtcaccg
    26941 ccaaaggtgg actggtggaa gaagagtacg tcgactgggt gatctcgcgg ccctacatct
    27001 tccttgtcct gctcaacctg gtgggcgttg cggtaggcat ctggcgctac ttctatggcc
    27061 cgccaaccga gatgctcacc gtggtcgtca gtatggtgtg ggtattctac aacctgattg
    27121 ttcttggcgg cgcagttgca gtatcggtag aaagcaaaca ggtacgccga tcgcaccgcg
    27181 tggagatgac gatgcccgcg gcaattgccc gcgaagatgg tcacctcttc tcgtgtaccg
    27241 ttcaggattt ctccgacggt ggtttgggga tcaagatcaa cggtcaggcg cagattctgg
    27301 aagggcagaa agtgaatctg ttgcttaaac gcggtcagca ggaatacgtc ttcccgaccc
    27361 aggtggcgcg cgtgatgggt aatgaagttg ggctgaaatt aatgccgctc accacccagc
    27421 aacatatcga ttttgtgcag tgtacgtttg cccgtgcgga tacatgggcg ctctggcagg
    27481 acagctaccc ggaagataag ccgctggaaa gtctgctgga tattctgaag ctcggcttcc
    27541 gtggctaccg ccatctggcg gagtttgcgc cttcttcggt gaagggcata ttccgtgtgc
    27601 tgacttctct ggtttcctgg gttgtatcgt ttattccgcg ccgcccggag cggagcgaaa
    27661 cggcacaacc atcggatcag gctttggctc aacaatgatg ataacgcgat gaaaagaaaa
    27721 ctattctgga tttgtgcagt ggctatgggg atgagtgcgt tcccctcttt catgacgcag
    27781 gcgacgccag caacgcaacc actgatcaat gctgagccag ctgtagccgc ccagacggaa
    27841 caaaatccgc aggtggggca agtgatgccg ggcgtgcagg gcgctgatgc gccagtcgtg
    27901 gcgcagaacg gtccttcgcg tgatgtgaag ctgacctttg cgcaaattgc accgccgccg
    27961 ggcagcatgg tgctacgtgg cattaacccg aacggcagca ttgagtttgg tatgcgcagc
    28021 gatgaagtgg tgacgaaggc gatgctcaac ctcgaataca cgccatcgcc atcgttactg
    28081 cctgtccagt cgcagttaaa ggtttatctc aatgatgagc tgatgggcgt gctgccagtg
    28141 accaaagaac agttgggtaa aaaaacgctg gcgcaaatgc ccattaaccc actgtttatt
    28201 accgacttca accgtgtgcg gctggagttt gtcggccatt atcaggacgt gtgcgaaaac
    28261 ccggccagca ccacgctttg gctggatgtt gggcggagca gtggactgga tctgacctat
    28321 cagaccctga atgtgaagaa tgacctgtca cacttcccgg tgccattctt tgacccgcgc
    28381 gataaccgta ctaacacctt gccaatggtc tttgcgggtg cgccggatgt tgggctgcaa
    28441 caagcctctg ccattgtcgc ctcgtggttt ggttcgcgtt ctggctggcg tgggcagaac
    28501 ttcccggtgc tctataacca actgccggat cgtaatgcca ttgtctttgc aaccaacgac
    28561 aaacggccgg acttcctgcg cgatcatccg gcggtaaaag cccctgtgat tgagatgatt
    28621 aaccatccgc agaatcctta cgtcaaactg ctggtggtgt ttggtcgtga cgacaaagac
    28681 ctgttgcagg cagcgaaagg tatcgctcag ggtaacattc tgttccgtgg tgaaagcgtg
    28741 gtagtgaatg aagtgaaacc gctgctaccg cgtaagccgt acgatgcgcc gaactgggta
    28801 cgtaccgatc gtccggtcac atttggcgaa ctgaaaacct atgaagaaca gttacaatcc
    28861 agcggtcttg agccagcagc gattaacgtt tcgctaaacc tgccgccgga tctctacctg
    28921 atgcgcagta ccggcattga tatggatatt aattaccgct acaccatgcc gccggtgaaa
    28981 gacagttcgc ggatggatat cagcctgaat aaccagttcc tgcaatcctt caacctgagc
    29041 agcaaacagg aggcgaaccg cctgctgctg cggattccgg tattacaagg tttgctggat
    29101 ggcaaaacag atgtctctat tccggcgctg aaactgggcg cgaccaacca gctgcgcttc
    29161 gactttgagt atatgaaccc gatgccaggc ggttcggtag ataactgtat taccttccag
    29221 ccggtgcaga atcatgtggt gattggtgac gattccacca tcgacttctc gaagtattac
    29281 cacttcatcc cgatgccgga tctacgcgcc tttgctaacg cgggcttccc attcagccgg
    29341 atggcggatc tgtcgcaaac catcaccgtg atgccgaaag cgcctaacga agcacagatg
    29401 gaaacgttgc tgaatactgt tggttttatc ggcgcacaga cgggcttccc ggcgattaat
    29461 ctgacggtga ccgatgatgg cagcaccatt cagggcaaag atgccgacat catgatcatc
    29521 ggtggtatcc cggacaaact gaaagacgat aagcagatcg acctattggt gcaggcgacc
    29581 gaaagctggg tgaaaacacc gatgcgccag accccgttcc ccggcattgt gccggacgag
    29641 agcgatcgcg cggcagaaac ccggtcaacg ctgacctctt ccggtgcgat ggcggcggtg
    29701 attggcttcc agtcgccgta taacgaccag cgcagcgtga ttgcgctgtt ggcagatagc
    29761 ccacgcggtt atgaaatgct taacgatgcg gtgaacgata gcggcaaacg cgccaccatg
    29821 ttcggttcgg tcgcggtgat ccgcgagtcc ggtatcaaca gcctacgtgt tggcgacgtt
    29881 tattacgtag gtcatctgcc gtggttcgag cgcttgtggt atgcgctggc aaaccatccg
    29941 attctgctgg cggtgctggc ggctatcagt gtgatattgc tggcatgggt actgtggcgt
    30001 ctgctgcgaa ttattagtcg tcgtcgtctt aacccggata acgagtaatt gaagatgaat
    30061 gtgttgcgta gtggaatcgt gacgatgctg ctgttggctg cctttagtgt tcaggcagcc
    30121 tgtacctggc ctgcctggga gcagtttaaa aaggattaca tcagtcagga agggcgcgtc
    30181 atcgacccca gcgacgcgcg caaaatcacc acctccgaag ggcaaagtta cggtatgttc
    30241 tttgccctgg cggctaacga ccgtgtagct ttcgataata ttctcgactg gacgcagaac
    30301 aatctcgctc agggctcttt aaaagaacgt ttgcccgcct ggctgtgggg caagaaagag
    30361 aacagtaagt gggaagtgct ggacagcaat tcggcctccg atggtgatgt ctggatggcc
    30421 tggtcgttgc tggaggcggg gcgtttgtgg aaagagcagc gttataccga catcggcagc
    30481 gcgttgctaa aacgtatcgc gcgggaggaa gtggtgacgg tgcctgggct gggttccatg
    30541 ttgttaccgg gcaaagtggg ttttgctgag gataacagct ggcgttttaa ccccagctac
    30601 ctgccgccga cgctggcgca gtatttcacc cgctttggcg cgccgtggac cacgctgcgc
    30661 gaaaccaatc aacgtttatt gctggaaacc gccccgaaag gtttttcgcc agactgggtg
    30721 cgctatgaga aagacaaagg ctggcagcta aaagccgaaa aaacattgat cagcagctac
    30781 gacgctatcc gcgtttacat gtgggtaggc atgatgcctg acagcgatcc gcaaaaagcg
    30841 cggatgctca accggtttaa accgatggcg acattcactg agaaaaacgg ttatccgccg
    30901 gaaaaagtgg atgtggctac ggggaaagcg cagggtaaag gaccggtcgg tttttctgcc
    30961 gccatgctgc cctttttaca aaaccgcgat gcgcaggccg ttcagcgcca gcgcgtggcc
    31021 gataactttc ccggcagcga tgcctattac aactatgtgc tgaccctgtt tggacaaggc
    31081 tgggatcaac accgtttccg cttctcgaca aaaggtgagt tattacctga ctggggccag
    31141 gaatgcgcaa attcacacta aacatattca cgctttccct cggtctggcc gtcatgccga
    31201 tggtcgaggc agcaccaacc gctcagcaac agttgctgga gcaagttcgg ttaggcgaag
    31261 cgacccatcg tgaagatctg gtgcaacagt cgttatatcg gctggaactt attgatccga
    31321 ataacccgga cgtcgttgcc gcccgtttcc gttctttgtt acgtcagggc gatattgatg
    31381 gcgcgcaaaa acagctcgat cggctgtcgc agttagcgcc gagttcaaat gcgtataaat
    31441 cgtcgcggac tacgatgcta ctttccacgc cggatggtcg tcaggcactg caacaggcac
    31501 gattgcaggc gacgaccggt catgcagaag aagctgtggc gagttacaac aaactgttca
    31561 acggtgcgcc gccggaaggt gacattgctg tcgagtactg gagtacggtg gcgaaaattc
    31621 cggctcgccg tggcgaagcg attaatcagt taaaacgcat caatgcggat gcaccgggca
    31681 atacgggcct gcaaaacaat ctggcgctat tgctgtttag tagcgatcgc cgtgacgaag
    31741 gttttgccgt cctggaacag atggcaaaat cgaacgccgg gcgcgaaggg gcctctaaaa
    31801 tctggtacgg gcagattaaa gacatgcccg tcagtgatgc cagtgtgtcg gcgctgaaaa
    31861 aatatctctc gatctttagt gatggcgata gcgtggcggc tgcgcaatcg caactggcag
    31921 aacagcaaaa acagctggcc gatcctgctt tccgcgctcg tgcgcaaggt ttagcggcgg
    31981 tggactctgg tatggcgggt aaagccattc ccgaactaca acaggcggtg cgggcgaacc
    32041 cgaaagacag tgaagctctg ggggcgctgg gccaggcgta ttctcagaaa ggcgatcgcg
    32101 ccaatgcagt ggcgaatctg gaaaaagccc tcgcactgga cccgcacagc agcaacaacg
    32161 acaaatggaa cagtctgctg aaagtaaacc gctactggct ggcgatccag cagggcgatg
    32221 ctgcgctgaa agccaataat cctgaccggg cagaacgcct gttccagcag gcgcgtaatg
    32281 tcgataacac cgacagttat gcagtgctgg ggctgggcga tgtggcgatg gcgcgaaaag
    32341 attatcccgc cgccgaacgt tattatcagc agaccttgcg tatggacagc ggcaacacta
    32401 acgccgtgcg cgggctggca aatatttacc gccagcaatc gccagaaaaa gctgaagcgt
    32461 ttatcgcctc gctctctgcc agtcagcggc gtagcattga tgatatcgaa cgcagcctgc
    32521 aaaacgaccg tctggcacag caggcagagg cactggaaaa ccagggcaaa tgggcgcagg
    32581 cggcagcact tcagcggcaa cgactggcgc tggaccccgg cagcgtatgg attacttacc
    32641 gactttcgca ggatctctgg caggccggac aacgcagcca ggccgatacg ttaatgcgca
    32701 atctggcgca gcagaagccg aacgacccgg agcaggttta cgcttacggg ctgtacctct
    32761 ctggtcatga ccaggacaga gcggcgctgg cgcatatcaa cagcctgccg cgcgcgcagt
    32821 ggaacagcaa tattcaggag ctggttaatc gactgcaaag cgatcaggtg ctggaaaccg
    32881 ctaatcgcct gcgagaaagc ggcaaagaag ctgaagcgga agcgatgctg cgtcagcaac
    32941 caccttccac gcgtattgac ctcacgctgg ctgactgggc gcagcagcga cgtgattaca
    33001 ccgccgcccg cgctgcatat cagaatgtcc tgacgcggga gccaactaac gccgatgcca
    33061 ttctggggct gacggaagtg gatattgctg ccggtgacac agcggcggca cgtagccagc
    33121 tggcgaaact gcccgctacc gataacgcct cgctgaacac acagcggcgc gtggcgctgg
    33181 cgcaggcgca gcttggagat accgcagcgg cgcagcagac gtttaataag ttgatcccgc
    33241 aggcaaaatc tcagccaccg tcgatggaaa gcgcgatggt gctgcgcgac ggtgcgaagt
    33301 ttgaagcgca ggcgggcgat ccaaagcagg cgctggaaac ctacaaagac gccatggtcg
    33361 catccggtgt gactacgacg cgtccgcagg ataacgacac ctttacccga ctgacccgta
    33421 acgacgagaa agatgactgg ctgaaacgcg gcgtgcgcag cgatgcggcg gacctctatc
    33481 gccagcagga tcttaacgtc accctcgagc atgattactg gggttcgagc ggcaccggtg
    33541 gttactccga tctgaaagcg cacactacca tgttgcaggt ggatgcgccg tattctgacg
    33601 ggcggatgtt ctttcgcagt gatttcgtca atatgaacgt cggcagtttc tccactaatg
    33661 ccgatggtaa atgggatgat aactggggca cctgtacatt acaggactgt agcggcaacc
    33721 gcagccagtc ggattccggt gccagcgtgg cggtcggctg gcgaaatgac gtctggagct
    33781 gggatatcgg taccacgccg atgggcttca acgtggtgga tgtggtcggc ggcatcagtt
    33841 acagcgatga tatcgggccg ctgggttaca ccgttaacgc ccaccgtcgg cccatctcca
    33901 gttctttgct ggcctttggt gggcaaaaag actccccgag caataccggg aaaaaatggg
    33961 gtggcgtacg tgccgacggt gtggggctaa gtctgagcta cgataaaggt gaagcaaacg
    34021 gcgtctgggc atcgcttagt ggcgaccagt taaccggtaa aaatgtcgaa gataactggc
    34081 gcgtgcgctg gatgacgggc tattactata aggtcattaa ccagaacaat cgccgcgtca
    34141 caatcggcct gaacaacatg atctggcatt acgacaaaga tctgagtggc tactcactcg
    34201 gtcagggcgg ttactacagt ccgcaggaat acctgtcgtt tgccataccg gtgatgtggc
    34261 gggagcgcac ggaaaactgg tcgtgggagc tgggggcgtc tggctcgtgg tcgcattcac
    34321 gcaccaaaac catgccgcgt tatccgctga tgaatctgat cccgaccgac tggcaggaag
    34381 aagctgcgcg gcaatccaac gatggcggca gcagtcaggg cttcggctac acggcgcggg
    34441 cattacttga acgacgtgtt acttccaact ggtttgttgg cacggcaatt gatatccagc
    34501 aggcgaaaga ttacgcaccc agccatttcc tgctctacgt acgttattcc gccgccggat
    34561 ggcagggtga catggattta ccgccgcagc cgctgatacc ttacgccgac tggtaagttt
    34621 tcagatagcg cctctcttaa tgccgctgcg atcgggtata ctcgggcggc aatctgggat
    34681 ttccgggggg agacaatttg cgcgtaagtc gctcgttaac aatcaagcag atggcaatgg
    34741 tggcagccgt tgtcctggtg ttcgttttta ttttttgcac cgttttgctg ttccatctgg
    34801 tccagcagaa tcgctataac acggctacgc aactggaaag cattgctcgc tctgtccgcg
    34861 aacccttatc ttcagctatt ttgaaaggcg atattcccga agcggaagct attcttgcca
    34921 gcattaaacc ggcaggcgtg gtcagccgtg ccgatgtagt gctgcctaac cagttccagg
    34981 cgctgcgtaa aagttttatt ccagagcgcc cggtgccggt aatggttact cgcctgtttg
    35041 agctaccggt tcaaatctcg ctgggcgttt actcgctcga acgtccggca aacccgcagc
    35101 caattgccta tctggtacta caggcggatt ccttccgtat gtataagttc gtgatgagca
    35161 ccctctcaac gttagtgacc atttacttac ttttgtcgct tatcctgacc gtcgccatca
    35221 gctggtgcat taaccgcctg attttgcatc cgttacgcaa tattgctcgc gaacttaacg
    35281 ccatcccagc ccaggagctt gttggtcacc aactggcatt accgcgtctg catcaggacg
    35341 atgaaatcgg tatgttggtg cgcagttaca acctcaacca gcaattgctg cagcgccatt
    35401 atgaagaaca gaacgaaaat gcgatgcgct tcccggtgtc ggatttgccg aacaaagcct
    35461 tgctgatgga gatgctggag caagttgtcg cgcgtaaaca aaccaccgcg ctgatgatca
    35521 tcacctgtga aaccctgcgt gatactgcgg gcgtgctgaa agaggcgcaa cgagaaattc
    35581 tgctgctgac gctggtggaa aaactcaaat cggtactgtc gccacgtatg atcctcgcgc
    35641 agattagcgg ttatgacttt gctgtcattg ccaacggtgt acaggaaccg tggcacgcaa
    35701 tcaccttagg tcagcaagtg ctcactatca tgagcgagcg cctgccgatt gaacgtattc
    35761 aactccgtcc gcactgtagc attggcgtgg cgatgttcta cggcgatctc accgccgaac
    35821 agctttacag tcgcgctatt tctgcggcat ttaccgctcg ccataaaggc aagaatcaga
    35881 ttcagttctt tgatccgcag cagatggaag ccgcccagaa gcggttgacg gaagagagcg
    35941 atatccttaa tgcactggaa aatcatcagt ttgctatttg gttacagcca caggtcgaga
    36001 tgaccagcgg aaaactggtc agtgcggaag tgttactgcg tatccagcaa ccggatggca
    36061 gttgggacct gccggatggc ttaatcgatc gcattgagtg ctgtgggctg atggttaccg
    36121 tcggtcactg ggtgctggaa gagtcctgtc gattgcttgc agcctggcaa gagcgcggca
    36181 ttatgctgcc cttgtcggta aacctctctg cgctgcaact gatgcacccg aatatggtgg
    36241 cggatatgct ggaactgtta acccgctatc gcattcagcc gggaacactg attctggaag
    36301 tgacagaaag ccgacgtatt gacgaccctc atgctgcggt ggcaatcctc cgtccgctgc
    36361 gcaatgccgg agttcgggtg gcgctggatg atttcggcat gggctacgca gggctgcgtc
    36421 agctgcagca tatgaaatcg ttgccaatcg acgtactaaa aatcgacaaa atgtttgttg
    36481 aaggcttgcc ggaagatagc agcatgattg ctgcaattat catgctggcg cagagcctga
    36541 acttacaaat gattgccgaa ggcgtggaga ctgaagcaca acgcgactgg ctggcaaaag
    36601 cgggcgttgg tattgcccag ggcttccttt ttgctcgccc actccctatt gaaatcttcg
    36661 aagagagtta cctggaagaa aagtagctac cccaaactga ttacaaaact ttaaaaagtg
    36721 ctggtttgtg cgagccagct caaacttttt aacctttttg tttcaattat gatccaggta
    36781 catttctgtg atgttgtctg ggtgttattt taaggccgca ggtaccccat aaccttacaa
    36841 gacctgtggt tttactaaag gacaccctat gaaaacctct ctgtttaaaa gcctttactt
    36901 tcaggtcctg acagcgatag ccattggtat tctccttggc catttctatc ctgaaatagg
    36961 cgagcaaatg aaaccgcttg gcgacggctt cgttaagctc attaagatga tcatcgctcc
    37021 tgtcatcttt tgtaccgtcg taacgggcat tgcgggcatg gaaagcatga aggcggtcgg
    37081 tcgtaccggc gcagtcgcac tgctttactt tgaaattgtc agtaccatcg cgctgattat
    37141 tggtcttatc atcgttaacg tcgtgcagcc tggtgccgga atgaacgtcg atccggcaac
    37201 gcttgatgcg aaagcggtag cggtttacgc cgatcaggcg aaagaccagg gcattgtcgc
    37261 cttcattatg gatgtcatcc cggcgagcgt cattggcgca tttgccagcg gtaacattct
    37321 gcaggtgctg ctgtttgccg tactgtttgg ttttgcgctc caccgtctgg gcagcaaagg
    37381 ccaactgatt tttaacgtca tcgaaagttt ctcgcaggtc atcttcggca tcatcaatat
    37441 gatcatgcgt ctggcgccta ttggtgcgtt cggggcaatg gcgtttacca tcggtaaata
    37501 cggcgtcggc acactggtgc aactggggca gctgattatc tgtttctaca ttacctgtat
    37561 cctgtttgtg gtgctggtat tgggttcaat cgctaaagcg actggtttca gtatcttcaa
    37621 atttatccgc tacatccgtg aagaactgct gattgtactg gggacttcat cttccgagtc
    37681 ggcgctgccg cgtatgctcg acaagatgga gaaactcggc tgccgcaaat cggtggtggg
    37741 gctggtcatc ccgacaggct actcgtttaa ccttgatggc acatcgatat acctgacaat
    37801 ggcggcggtg tttatcgccc aggccactaa cagtcagatg gatatcgtcc accaaatcac
    37861 gctgttaatc gtgttgctgc tttcttctaa aggggcggca ggggtaacgg gtagtggctt
    37921 tatcgtgctg gcggcgacgc tctctgcggt gggccatttg ccggtagcgg gtctggcgct
    37981 gatcctcggt atcgaccgct ttatgtcaga agctcgtgcg ctgactaacc tggtcggtaa
    38041 cggcgtagcg accattgtcg ttgctaagtg ggtgaaagaa ctggaccaca aaaaactgga
    38101 cgatgtgctg aataatcgtg cgccggatgg caaaacgcac gaattatcct cttaatctca
    38161 tcacttatgc ccgcactccc ttccccgagt gcgggtgttt gcgcataatt gacccctgtg
    38221 cctgaactta ccacctgggt gagttcaggt ttatttcact tcttccgtga cattttcatg
    38281 ttcttgcggt ctaacacgaa gtgtttttaa cgtcatttag actgaccgac aacgggcagt
    38341 ttttttatta ccaggatagt tgatcagggg ttcacatgca gggcacaaaa attcgacttt
    38401 tagcgggcgg tttgctgatg atggccactg ctggctatgt gcaggcagat gcgctccagc
    38461 ctgatccagc atggcaacag gggacgcttt ccaacggttt acagtggcaa gtgctgacca
    38521 ccccccagcg tcccagcgat cgtgttgaaa ttcgcctgct ggttaatacc ggttcgctcg
    38581 ccgaaagtac acaacagagc ggttacagtc acgccatccc tcgtattgcg ctaacgcaaa
    38641 gcggtggcct tgacgcagca caggcgcgtt cattgtggca gcaggggatc gaccctaaac
    38701 gcccgatgcc gccggtaatt gtctcgtatg acactacgct gtttaatctg agtttgccca
    38761 ataaccgtaa cgatttgctg aaagaggcgc tctcttacct ggcaaatgcc actggcaaac
    38821 tgactatcac gccagaaacc atcaaccacg cgctgcaaag tcaggacatg gtggcaacct
    38881 ggcctgccga tactaaagag ggctggtggc gttatcgtct gaaaggatca accttgttag
    38941 gtcacgatcc tgccgatccg ctgaaacaac ccgttgaagc ggaaaaaatt aaagatttct
    39001 atcagaaatg gtacaccccg gatgcaatga cgctactggt ggtgggaaac gtggatgcgc
    39061 gctcggttgt cgaccaaatc aacaaaacgt ttggcgaact gaaaggcaaa cgtgaaacgc
    39121 cagctccggt gccgacgctt tctccgctgc gtgcggaagc ggtgagtatt atgactgacg
    39181 cggtgcgtca ggaccggtta tctatcatgt gggatacgcc gtggcagccg attcgtgaat
    39241 cagccgcact gctgcgctac tggcgtgcgg acctggcccg cgaggcgctg ttctggcatg
    39301 ttcagcaagc gttaagcgcc agtaacagca aagacatcgg tcttggattt gactgccgtg
    39361 tgctgtatct gcgtgcgcag tgtgccatca acatcgaatc accaaacgac aagctgaaca
    39421 gcaaccttaa tctggtggcg cgtgaactgg cgaaggttcg cgataaaggt ctgccggaag
    39481 aagagttcaa tgcgttagtg gcgcaaaaga aactggagct gcagaaactg tttgccgcct
    39541 atgcgcgggc tgataccgat attctgatgg gtcagcggat gcgttcgttg caaaatcagg
    39601 tcgtcgatat cgcgccggag cagtatcaga aactgcggca ggatttcctt aacagcctga
    39661 cggtggagat gttaaatcag gatctgcgtc agcagttgtc gaatgatatg gcgttaatac
    39721 tgctgcagcc gaaaggcgag ccggaattta acatgaaagc gttgcaggcg gcctgggatc
    39781 aaatcatggc cccatctacc gccgctgcga ccacctctgt cgccacggat gacgtacatc
    39841 ctgaagtgac ggatattcca cctgcacagt aatgcctgat ggtgatgagt tagcacaatc
    39901 atcaggtatt tatggatgag ctggatagag gggtaacact ttatccagcc ttttgcatat
    39961 gctgctttta cgctggcatc gcctcacgcg ggataatcgc gccgcgatac tgaataacgg
    40021 tacttgcggt caggtgcccg cgtttcgccg cgttttccgc actgccgccc gttaaacgta
    40081 ccgccagata accggcactg aaagagtcgc cagctgcggt ggtatcgatc actttttctt
    40141 tcggcagttt caccgccgga acatccacta acccttcgcc agcaatggac acaaggcaag
    40201 aatccgcccc gcgtttcacc accacttctt tcacgcccgc gttatgggtg cgcgcaatga
    40261 cgtcttccac cggctgttga ccccacagcg cgtcttcgtc gtccagcgtc aggaaggcga
    40321 tatccgtgca ttccagcatt tgttggtaca cctgctgtgt ctcttctttg ctggcccaca
    40381 ggcgcggacg atagttattg tcgaaaatca cttttccgcc gttggcgcgg cattcgcgca
    40441 gcagggaaag cagcttttcg cggctggtcg ggcttaagat cgccaggcta atcccgctca
    40501 ggtagagata atcgaaattc gccagctctt cgcaaatcgc cgcagactgc tcgctctcca
    40561 gccagaattt ggcagcggct tcgttacgcc agtagtagaa cgtacgttca ccggtgctgt
    40621 cggtttcaat gtagtaaagg cccggcagac ggttttccat ccgttgggtc agggaagtat
    40681 caacgttctc gccgtgccag gcgtccagca tttgctggct aaaactgtcc gttcccagcg
    40741 ccgttacgta atggacggtt aatgccgcag gatcgacctg acgggcgata tagacggaag
    40801 tgttcagggt atcgccgccg aaaccgcgct taacgtccgc gcctttctcg gaaagctcaa
    40861 tcatgcattc gccaatcacg gcaatctttt tggacatagt cgtgaacctg atctgtaaaa
    40921 aattagcgtt agtttgcgct gtggtggttt gctggtcaac catattaaaa cagtgttcca
    40981 ttattttttt gagccaggac gtattcctga aagattccgt tgtggagtga ggaaatttac
    41041 cccggccagg cggtaaagtt ctgcccttac gcgccgataa tctttgtcga gtccgggcag
    41101 catcactttt aaacacagga catctttgat gataaggcag gttatccagc gaataagcaa
    41161 ccctgaagca agcatcgaga gcttgcagga acggcgtttt tggttgcagt gtgagcgtgc
    41221 ttacacctgg cagccgatct atcaaacatg cgggcggtta atggccgtgg agctattaac
    41281 ggtggtcgcg catcccttga acccttcgca acgcctgccg ccggatcgct attttactga
    41341 aatcaccgtc agccatcgga tggaggttgt gaaagagcag attgatttgc tggcgcaaaa
    41401 agccgacttc tttatagagc acggcctgct ggcatcggtc aatattgatg gccctacgct
    41461 catcgccctg cgtcagcaac caaaaatcct gcgccagatt gagcgtcttc cctggctgcg
    41521 tttcgaactg gtggagcata tccgtctgcc gaaagattca acctttgcct cgatgtgtga
    41581 atttggcccg ctgtggctgg atgattttgg taccgggatg gcaaatttct ctgcactaag
    41641 tgaagtgcgt tatgactaca tcaaaatcgc gcgagaactg tttgtgatgc tgcgtcagtc
    41701 gccggaagga cgcacactct tttctcagct tttacatcta atgaatcgct attgtcgcgg
    41761 ggtgattgtt gagggcgtag aaacgccgga agagtggcgt gatgttcaga actcgcccgc
    41821 attcgccgca caaggctggt ttctttcacg cccggcaccg atagaaacgc tgaatacggc
    41881 ggttctggcg ctataagctg cctcattttc cgcctggctg gactatcttt aggactggca
    41941 caggaagaat atgaggaagc gaacgatgag caaggcaggc aaaataaccg ctgcgatttc
    42001 aggggctttc ttgttgttga ttgtcgtggc gatcattttg attgcaactt ttgactggaa
    42061 tcgactcaaa ccgaccatca accagaaagt ctctgcggag ttgaatcgtc cgttcgctat
    42121 ccgtggcgat ctgggcgtgg tgtgggagcg gcaaaagcaa gaaacaggct ggcgcagctg
    42181 ggtgccgtgg ccccatgtac acgcggaaga catcattctt ggcaatccac cggatattcc
    42241 cgaagtcacg atggtgcatt tgccacgcgt agaggcaacg ctggccccgc tggcgctgct
    42301 gaccaaaacg gtctggctgc cgtggatcaa gctcgaaaag cccgacgcgc gcctgattcg
    42361 cctctccgaa aagaacaata actggacgtt taatctcgcc aacgatgata acaaagacgc
    42421 gaatgcaaag ccgtcggcat ggtcgtttcg gctggataat attcttttcg atcaagggcg
    42481 gatcgccatt gatgacaaag taagcaaagc ggatctggag atttttgttg atccgttagg
    42541 caagccgctg ccgttcagcg aagttactgg atcgaaaggt aaagcggata aagaaaaggt
    42601 gggcgattac gtttttggcc tgaaggcgca gggacgttat aacggtgaac cgctcacggg
    42661 tacgggaaaa ataggcggta tgctggcgct gcgtggcgaa gggacgccgt ttccggtaca
    42721 ggctgatttc cgctctggta acacccgtgt tgcttttgat ggcgtcgtga atgacccaat
    42781 gaagatgggc ggtgtcgatt tacggcttaa attttctggc gattcactgg gtgatctcta
    42841 tgaactgacg ggcgttctgc tgcccgatac cccgccgttt gaaacggatg gtcggctggt
    42901 agcgaaaatc gacactgaaa aatcgtcggt ctttgattat cgcggtttta atgggcgaat
    42961 tggcgatagc gatatccacg gttctctggt ctacaccacc ggaaagccac gaccaaaact
    43021 ggaaggtgat gtcgagtcgc ggcaattgcg gctggcggac ctgggaccgt taattggcgt
    43081 tgactccggg aaaggggcag aaaagtcgaa acggtctgaa cagaagaagg gcgaaaaaag
    43141 cgttcagcct gcgggcaaag tgctgcctta tgaccgcttc gaaaccgata aatgggacgt
    43201 tatggatgcc gatgttcgct tcaaagggcg gcgcattgag catggcagta gcctgccgat
    43261 tagcgatctt tctactcata tcatcctcaa aaatgctgac ctgcgcctgc aaccgctgaa
    43321 atttggcatg gcgggcggca gcattgcggc gaatattcat ctggaaggcg ataaaaagcc
    43381 gatgcagggg cgggcagata ttcaggctcg tcgactgaaa ctgaaagaac tgatgcccga
    43441 tgtggaactg atgcagaaga cgctggggga aatgaacggt gacgcggaac tacgcggtag
    43501 cggtaactcg gtggcagcac ttttaggcaa cagtaacggc aacctgaaac tgttgatgaa
    43561 tgacgggctg gtgagccgca acctgatgga gattgttggg ctgaatgtcg gcaactacat
    43621 tgtcggtgcg atatttggtg acgatgaggt gcgggtgaac tgcgcggcgg cgaatctgaa
    43681 tattgccaac ggcgtggcgc gcccgcagat ttttgctttc gatactgaga acgcgttgat
    43741 taatgttacc ggcacggcaa gttttgcttc ggaacagctg gatttgacta ttgatccgga
    43801 gagtaaaggg attcggatta tcacactgcg ttcgccgctg tatgtgcgtg ggacgtttaa
    43861 aaatccgcag gctggggtga aagccgggcc gttgattgcc cgtggtgctg ttgctgcggc
    43921 actggcaacg ctggtaacac cggcggcggc gttactggca ctgatctcac cttccgaagg
    43981 ggaggctaat cagtgtcgga cgatattgtc gcagatgaag aagtgatgag gttggattgt
    44041 gtttgctggt gagttcggga gcagcgtgtt cccctcaccc taaccctctc cccaaagggg
    44101 cgaggggact gttttgtgcg tgttgaaaca acgagtaccg cttttccgtt cactttctgg
    44161 gggagggctg ttatgttcgc gatataacat ctcgctccgc ttttctccct ctccctttca
    44221 gggagagggc tggggtgagg gtggctgccc acacgatact tacaacgact gatgtcgcgt
    44281 ctcatgggtc agcagcaggg cgattaacgt caagccagcc atcgccgcca gatataaccc
    44341 caccgcacct aacccgtagt tagcctgcaa ccaggctgcg atatatggtg caacggaagc
    44401 cccgagaatc gacgctacgt tgtaagagaa cgatgctccg gtgtaacgca cttctgtcgg
    44461 gaacagctct ggtaacagcg cgcccatcgg cccgaaggtc agtcccatca gacttaaccc
    44521 cagcagcagg aaggcaaaaa ccagaatcgg gttgccagaa ccgagcagcg ggttaaaggc
    44581 gaacagcgcg aacaggatga tcagcgtggt gatgattacc atgcttttac ggcgaccaaa
    44641 ggcatcagcc agtaatccgg cgactggcac catcacgcca aaaccaataa ctgccatcat
    44701 cagcatccac aacacttcgt tacgcggcag gccaagccca actggcgcgg cggcggtact
    44761 gaaggtcata gagtagaccg tcatgatgta aaacagcgta taggttgcca gcataatgaa
    44821 cgtacccagt acggttacgc gaacatgttt ggtcagcagc gtacccagcg ggatcttcac
    44881 ctgttttttc gctttagcga ctttctcaaa caccggcgac tcatgcagcg acacgcgaac
    44941 atacaggccg ataatgacca gcaccgccga gaagataaat ggcacacgcc agccccagct
    45001 cataaactgc tcgtcggtca gtagccagga aagcagcaag aaagtgccgt tggcaaagaa
    45061 gaagccaatc ggtgcgccca gctgtgggaa ggagccatac agtgcacgtt tgcgcggtgg
    45121 ggcgttttca gtcgccagca gcgccgcgcc gccccattca ccgcctaagc ccagaccctg
    45181 accaaatcga gccagcgcca gcagcagcgg ggcgaaaata ccaatcgtgg catagcccgg
    45241 cagcagacca atcaccacgg tcgaaatccc catcgttagc aacgaggcga ccagcgtcgc
    45301 tttacgccca acgcgatcgc caaaatgacc aaaaacggca gagccaatgg ggcgcgcgac
    45361 gaaggcgatg gcgaaggtgg cgagcgactg tagcgttgct gccgttggat cgccctgcgg
    45421 gaagaagata tgcggaaaca caataacggc cgcagtggcg taaatgtaaa agtcgaagaa
    45481 ctcaatggcc gtgccaatga gagaggcgac aaggacttta ttacgcgagt tgattggcgt
    45541 gtattcttgc tcgtggtcga gtgttgtggc tgttgcttgc ataatctttt cttatttttt
    45601 ggctaacgaa tagccatatt acgcacagca aaagtgatat ttcaatgtgt aacaaatcgc
    45661 ggaaatggtc aaaaaccagg caaaaagggg ataatgttta ggccagtgat ttttcttcta
    45721 tgaaatgctt cacataattt tataaatagt ggataaaact ggtttcaggt taaataaaag
    45781 ttaccaactg gatttatatg ctggggttat gagttggaat gaaattgttt gctcccaaat
    45841 agatggctga agaggctctg gggaagagtt tcaagaggtt atgtgcgcat tatagccgga
    45901 tgcgatgctg gcgcatctta tccggcctac ggggagccag gccgaataaa atcgtatgcc
    45961 ggataaggcg tttacgccgc atccggccta ttttaaggct gcgttttccc tggcattcgc
    46021 gggtcgtctt tatattcggc ggtggcaatc cacgccgcgc aaaacagcgt caaacgggcg
    46081 aagaagtaga aaaatgccat cagccccagc acggaaccaa atgctgcgcc agagggggat
    46141 ttcatcagcg atggcaaggt gtaggtcatc acgattttaa tcacttcaaa accaatagca
    46201 gccagaaatg ttccgcgaat cagggctttt ttgcgcggac ggtggcgcgg caaccgccag
    46261 aagatccaga agaaaagcag atagttggcg aagatggaaa tcgccaggcc aatcaaccgc
    46321 cacgtcggtt tcagccactc aatgctgttc aggtgcaggg cgctaataat catttgctgc
    46381 gccgaaccgg caaccgaggt gatcgacagc gtcacaatca gcgcaattaa caaaccaatc
    46441 agtgaaataa aatcacgcag atattttacc cagaacttct cctgatcttg cggcgagcgt
    46501 tcccagacat cgcgcgactg ggcacgaatc gcttcacgca ggttacccat ccagttgatg
    46561 ccagaataaa gcgccaccgc caggccgaca agccctacag tcgtacgctg ctgaacggcg
    46621 gtgttgatgg tgtttttcaa cgtggcggct agcgtcggat cgctgatgtt ttgcagaatt
    46681 ttgtcgaaga tatcctgtag caacatcgga tgggaggcca gcacaaagcc ccctgcggca
    46741 aacgacacca tcaaaatcgg gatcatcgat aaaaatgaga aataggtgat agccgcacca
    46801 aactggttac ccagccgatc gttaaagcgt tctgtcgcgc gaatcaggtg cgcaataacc
    46861 ggttggcgtt ggactttttc ggcagtggtg gtgacggttt ccagcgcctg gctaactttg
    46921 ccgcctttct cgctattatc caactgctta atcggctcgt gctccagatc ctgggtggga
    46981 cgtttgatct cgttttcctg cgtcatccgc tcttttgtcc ttctgtcgtg gtcattctta
    47041 aaaagtatag tcggtcagtc caggtgctct ttcattacgc cagccagcca ctgcataaac
    47101 aggtttacac gccgggaaag ctcccgacgc tgcgggtaaa ccagcgaaag ggagagcggc
    47161 tcggcacggt agcctggcaa cacttcaata agccgcccgg cacgcagggc ttcgcgcacg
    47221 gcaatgcgcg ggatctgaat aatccccaga ccggcaagac cggcggcgag atagttttcg
    47281 ctactgttta ccgtcaacat gccgccagac ttaaaccact ggacgccatt aacgctggca
    47341 acctcaaaac ctaacggatg tacacccagg tgcggtgtat aacgcactat tgcgtgtgaa
    47401 gtcagatcat cggggctttg cggataacca aagcgcgtca gatagtgcgg actggcacag
    47461 ttgaccatgg tcagtttgcc gaggggacgg gcgataacgc catcttccgg taacgcgcca
    47521 gtgcgtatca cgcaatcaaa accatcatga agaatatcta ccggacggtc atggctactc
    47581 agttccagct caattcccgg atgcagatag agaaattccg acaggcgcgg cagtaacagg
    47641 cttttcgcga ttcctggcgg gatgtcgatg cgtaatttac cgctgatact ggtggcatcc
    47701 tgttgaaaca gaccgtccag ttcgctgaga ttactcaaca catcttttgc tcgttgataa
    47761 taggtcatgc cttctggcgt gagtttgacc cgtcgcgtgg tgcgctggag aagctgggtg
    47821 ccaagttgat gttccagtgc ctgtatctgg cgcgaaacac ttccttttgg caaagcaaag
    47881 aaatccgccg cgcgggaaaa actttccagc tccgcgactt tgatgaacaa ctgcattgcg
    47941 tgaattttat ccatttcttt gaccgattgt tgttcacagc gaaacagtaa cgcgcatatc
    48001 gcattgttta ttgttttttc taacatctaa tgccgggttt cacaatgttg tcatgacgaa
    48061 tgtggcgatt tctcagcagg aaaagggcgg aatagctttc tgagaaaggt gaatccccct
    48121 aaacagagcg tatttttcag cgattccctt taaagtgaat agaggacgtg ttggctattt
    48181 gtgcttattt ttgtctcatt gccgtttgtt atttttgctt gtggtcgcgg agtttcattt
    48241 ttaattgcat gcgtactaac caccaatata aggaaatcct caaaattgtt ttaaatagtg
    48301 ccctcgacat gtaatttcag gcgtttagct gtaactattt atatcggtaa ctattttcta
    48361 cgcgtcatct ctgtagaatg gccaccgcca aatggcgaat tcttattttt tctcgcgggt
    48421 aatacccttc cactacttaa gtctttttaa cgtattcagg tggagattca gttatgcaaa
    48481 tagtcatgtt tgacaggcag tcaatattta ttcatggaat gaaaatcagt ttacagcagc
    48541 gtattccagg agtgagtatt cagggggcca gtcaggcaga cgagttatgg caaaagctgg
    48601 aaagttaccc tgaagcctta gttatgctcg atggcgatca ggatggtgag ttttgctact
    48661 ggttgctgca aaaaaccgtg gtgcagtttc ctgaggttaa ggtgttaatc acggcgacgg
    48721 attgcaataa acggtggtta caggaagtta ttcattttaa tgtgctggcc attgtgcctc
    48781 gtgattcaac cgtcgagaca tttgcgttgg cggtaaatag tgcggcgatg gggatgatgt
    48841 ttctgcctgg agactggcgg acgacgccgg aaaaggacat taaagatctc aaatcgttaa
    48901 gcgcccgcca gcgggagatt ttaaccatgt tagcggcggg cgaatcaaat aaggagatcg
    48961 gcagagcgct gaatatcagt accggaacgg taaaagcgca tctggaatcc ctgtatcgcc
    49021 gtctggaagt gaaaaatcgc acccaggcgg caatgatgtt aaatatctcc tcctgaagag
    49081 gataatttta cagccagcag gaagttagcg gctggctgta aaaatattat ggttcgccgt
    49141 acaaaccaat taaacggcgt accacaccgt tagtccagcc aaacccatcc tgcaacggat
    49201 actcgccacc gccgccttcg cggggaacac catcggcaat atggtatttc tcgatcattt
    49261 tgtgctgttc cagatagaac tgattcaccg ttttcagcca gctgcgcgcg atttcatcac
    49321 ccagaaggtc atcgccatac attttaaatc cctgaattgc catccattgt aacggtgccc
    49381 agccattggg tttatcccac tgttcaccgg tttcgtactc gcttgccaga atcccgccag
    49441 gtgtcagtaa ccggctgcgt acggcgtttg ccagacgatc ggcctgttca tggttcgcca
    49501 tgccgacata gagcggcaca atggcggcag cggaaaacag cgccagttgt tcgcgtcgcc
    49561 agtcgtaatc gcggtagatg ccgttttcat catcccagag gtaacggttt accgcatcgc
    49621 gacgggcact ggccttctgg cggaacagcg cttctgtctc tttctcgcct ttcagcgccg
    49681 agatgttggc gatggcgctc tccagtttaa acaggaaggc attcagatcg atggggatga
    49741 actgggtggt acgaatgctc gccagacgac cagtatcacg cagccaacgg gaagagtaat
    49801 cccaacctga ggccgctccc gcgcgtaaat cgcggtacac ctcgttgggc gggcgaccag
    49861 aatgtttcgc ggtttcaacg tcctcaagcc aggattcgtc acgcggcgtg tcgcgatcgt
    49921 cccaataacg gttgagcagc gatccgtccg gcatccgcac aacatggcga taggcctgat
    49981 tagggatcaa cgattctgca ccgtccatcc agaaggcata ttccatttta aggtggtcga
    50041 gatagcggcg cgcaccgcgt acaccatctt cttcaaacaa ctccaccatc agcgcaaaaa
    50101 ccggtggttg cgatcggctc aaataatagg tgcggttgcc gttggggatg tgaccatagt
    50161 tttcgatcat ccaggcgaag ttatcggcca tgcattttag caaatcttcc cgaccacttt
    50221 ccgccagccc cagcatggtg aaataggaat cccaatagta ggtttcgcta aaacggccgc
    50281 ccgggacaat atatgactgc ggtagcgcca gcagagaaga ccacggaatg tgatcctgtg
    50341 gttcgcgggt tagcaccggc cacagctggt cgatatgctc tttcagggaa ttttgcgggt
    50401 ccgatacata ctcgctggag tagacctccg gcagccagaa gtgattttca acaaacttgc
    50461 gcaagtcaaa atcacgatga cggcgcactt tacggtagcg gattaaaata tccagcgggt
    50521 ccattttggg tgcgcagtcg ggaaaggttt tactgtcggg gaaaattttc gccgactgaa
    50581 catgctcgaa cagttcgaga tagcgatcgg caggcgtcag cgcatcagag gcgggcagcc
    50641 cttcaatcat ctcgggttcc ggttctgcct cgatcatctc atccagtttt aattcatacg
    50701 ggtccagctc atagcagaga tcgacttcga tcatcagttc gtctggatta gggttttgaa
    50761 ttttctgatt gagcataagg atccaacctc cgaaattcgt cgctaattat cgggcttttc
    50821 tttaagggta gtcattcgct ccgcctggtg ggcgacaaag cgtgcggtgg atcacgttta
    50881 ttaacctgtc aaaaaatata acgcactaat atccatggaa tttataaaaa tacggatttc
    50941 gtgttgtttt tcattttcag aacattcaat cctgctctcg ccgcaatcat gatatgccat
    51001 tcctgatctg ggtaataggc acaggctatc ttattgatag tttatattca tgtaattgat
    51061 tgattcctat caaaaagcgt atctccctgt tttttatgat ggctttgtct cgcagagacg
    51121 tttgcagcat tctggaaaat tttctgggtc acaataactc accaccgtga gggaagaata
    51181 atgaaaatgg tctcacgtat taccgcgatc ggcctggctg gcgtcgcgat ttgctattta
    51241 gggttatctg gttatgtgtg gtaccacgat aataaacgca gtaaacaggc cgatgttcag
    51301 gcatctgctg tcagtgaaaa taataaggtt ttaggctttc tccgcgaaaa aggatgcgac
    51361 tattgccaca caccttcggc agaattaccc gcctattatt atattcctgg cgcgaaacag
    51421 ttgatggatt acgacattaa gcttggatat aaatctttta acctcgaggc cgtgcgtgcg
    51481 gcactgctgg ctgataaacc cgtttcgcaa agcgatctga ataagattga atgggtgatg
    51541 cagtatgaaa ctatgccacc aacgcgttat accgcgctac actgggcggg taaggtgagt
    51601 gatgaagagc gggcggaaat actggcctgg attgcaaaac agcgcgcgga atattacgcc
    51661 agcaatgata ctgctccgga gcatcgcaat gaaccggtgc agcccatccc gcaaaaactg
    51721 cctaccgatg cgcaaaaagt ggcgttgggc tttgcgctgt atcacgatcc ccgtttatcg
    51781 gctgatagca ccatttcatg cgctcattgc catgcgttga atgcgggggg cgtcgatggc
    51841 agaaaaacat cgattggtgt tggtggcgca gttgggccga ttaacgcgcc gacggtattt
    51901 aactcagtat ttaacgttga gcagttctgg gatggtcgtg cggcaacatt gcaggatcag
    51961 gcaggtggac cgccgttgaa cccgattgaa atggcgtcga aatcctggga cgaaattatt
    52021 gctaagctgg aaaaagatcc gcagcttaaa gcgcagttcc tcgaagttta tccgcaaggt
    52081 ttcagtggcg aaaatattac tgatgccatt gctgaatttg agaaaacatt aattacgccg
    52141 gattccccat ttgataaatg gttgcgtgga gatgaaaatg cgctgacggc gcaacagaaa
    52201 aaaggctatc aattatttaa agataataaa tgtgcaactt gtcatggtgg tattattctc
    52261 ggcggacgtt cctttgaacc gttggggctg aaaaaagact ttaactttgg ggaaattacg
    52321 gcggcggata ttggtcgtat gaatgtgact aaagaagagc gtgataaatt gcgtcagaaa
    52381 gtacccggtt tacgtaacgt tgctttaacg gcaccgtact tccatcgcgg tgacgtgccg
    52441 acgctggacg gggcggtgaa actgatgctg cgctatcagg taggcaaaga gctgccgcag
    52501 gaggatgtgg atgatatcgt agctttcctg cacagtctga acggggtgta cacgccgtat
    52561 atgcaggata aacaataatt aatttgatcg cccgaacagg aatgtttggg cgatttttat
    52621 tacgataata aagtctgttt ttaatattat catgttaaat gtttatatta taaaaagtcg
    52681 tttttctgct taggattttg ttatttaaat taagcctgta atgccttgct tccattgcgg
    52741 ataaatccta cttttttatt gccttcaaat aaatttaagg agttcgaaat ggaccagaag
    52801 ctgttaacgg atttccgctc agaactactc gattcacgtt ttggcgcaaa ggccatttct
    52861 actatcgcgg agtcaaaacg atttccgctg cacgaaatgc gcgatgatgt cgcattccag
    52921 attatcaatg atgaattata tcttgatggc aacgctcgtc agaacctggc cactttctgc
    52981 cagacctggg acgacgaaaa cgtccataaa ttgatggatt tgtcgatcaa
//
LOCUS       DAJOWB010000018        52577 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJOWB010000018 DAJOWB010000000
VERSION     DAJOWB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.018"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            8..439
                     /locus_tag="OHO85_001221"
     CDS             8..439
                     /locus_tag="OHO85_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCQ2670632.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKEIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFSCYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="OHO85_001222"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(586..2748)
                     /gene="uvrD"
                     /locus_tag="OHO85_001222"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCQ2670633.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="OHO85_001223"
     CDS             complement(2832..3548)
                     /gene="yigB"
                     /locus_tag="OHO85_001223"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCQ2670634.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="OHO85_001224"
     CDS             complement(3548..4444)
                     /gene="xerC"
                     /locus_tag="OHO85_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCQ2670635.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4441..5148)
                     /locus_tag="OHO85_001225"
     CDS             complement(4441..5148)
                     /locus_tag="OHO85_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCQ2670636.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMVLLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="OHO85_001226"
     CDS             complement(5145..5969)
                     /gene="dapF"
                     /locus_tag="OHO85_001226"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCQ2670637.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6006..6209)
                     /locus_tag="OHO85_001227"
     CDS             complement(6006..6209)
                     /locus_tag="OHO85_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCQ2670638.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6368..6510
                     /locus_tag="OHO85_001228"
                     /pseudo
     CDS             6368..6510
                     /locus_tag="OHO85_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            6672..6992
                     /gene="cyaY"
                     /locus_tag="OHO85_001229"
     CDS             6672..6992
                     /gene="cyaY"
                     /locus_tag="OHO85_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCQ2670639.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7032..9578)
                     /gene="cyaA"
                     /locus_tag="OHO85_001230"
     CDS             complement(7032..9578)
                     /gene="cyaA"
                     /locus_tag="OHO85_001230"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCQ2670640.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            9965..10906
                     /gene="hemC"
                     /locus_tag="OHO85_001231"
     CDS             9965..10906
                     /gene="hemC"
                     /locus_tag="OHO85_001231"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCQ2670641.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            10903..11643
                     /gene="hemD"
                     /locus_tag="OHO85_001232"
     CDS             10903..11643
                     /gene="hemD"
                     /locus_tag="OHO85_001232"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCQ2670642.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            11665..12888
                     /gene="hemX"
                     /locus_tag="OHO85_001233"
     CDS             11665..12888
                     /gene="hemX"
                     /locus_tag="OHO85_001233"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCQ2670643.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQQETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPQADTSAAAPQGE"
     gene            12891..14087
                     /gene="hemY"
                     /locus_tag="OHO85_001234"
     CDS             12891..14087
                     /gene="hemY"
                     /locus_tag="OHO85_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCQ2670644.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            14766..14927
                     /locus_tag="OHO85_001235"
     CDS             14766..14927
                     /locus_tag="OHO85_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670645.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(15011..16246)
                     /locus_tag="OHO85_001236"
     CDS             complement(15011..16246)
                     /locus_tag="OHO85_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCQ2670646.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDKFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVSK"
     gene            complement(16393..16469)
                     /locus_tag="OHO85_001237"
     tRNA            complement(16393..16469)
                     /locus_tag="OHO85_001237"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16433..16435),aa:Pro,seq:tgg)
     gene            complement(16512..16598)
                     /locus_tag="OHO85_001238"
     tRNA            complement(16512..16598)
                     /locus_tag="OHO85_001238"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16562..16564),aa:Leu,seq:cag)
     gene            complement(16619..16694)
                     /locus_tag="OHO85_001239"
     tRNA            complement(16619..16694)
                     /locus_tag="OHO85_001239"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16659..16661),aa:His,seq:gtg)
     gene            complement(16753..16829)
                     /locus_tag="OHO85_001240"
     tRNA            complement(16753..16829)
                     /locus_tag="OHO85_001240"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16793..16795),aa:Arg,seq:ccg)
     gene            complement(16932..18317)
                     /locus_tag="OHO85_001241"
     CDS             complement(16932..18317)
                     /locus_tag="OHO85_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCQ2670647.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(18508..19248)
                     /gene="rffM"
                     /locus_tag="OHO85_001242"
     CDS             complement(18508..19248)
                     /gene="rffM"
                     /locus_tag="OHO85_001242"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCQ2670648.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(19251..20603)
                     /gene="wzyE"
                     /locus_tag="OHO85_001243"
     CDS             complement(19251..20603)
                     /gene="wzyE"
                     /locus_tag="OHO85_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCQ2670649.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(20600..21679)
                     /gene="rffT"
                     /locus_tag="OHO85_001244"
                     /gene_synonym="wecF"
     CDS             complement(20600..21679)
                     /gene="rffT"
                     /locus_tag="OHO85_001244"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCQ2670650.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAIIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGYVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVLEAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(21676..22926)
                     /gene="wzxE"
                     /locus_tag="OHO85_001245"
     CDS             complement(21676..22926)
                     /gene="wzxE"
                     /locus_tag="OHO85_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCQ2670651.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(22928..24058)
                     /gene="rffA"
                     /locus_tag="OHO85_001246"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(22928..24058)
                     /gene="rffA"
                     /locus_tag="OHO85_001246"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCQ2670652.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(24063..24737)
                     /gene="rffC"
                     /locus_tag="OHO85_001247"
                     /gene_synonym="wecD"
     CDS             complement(24063..24737)
                     /gene="rffC"
                     /locus_tag="OHO85_001247"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCQ2670653.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNASDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASSRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(24715..25596)
                     /gene="rfbA"
                     /locus_tag="OHO85_001248"
     CDS             complement(24715..25596)
                     /gene="rfbA"
                     /locus_tag="OHO85_001248"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCQ2670654.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(25615..26682)
                     /gene="rffG"
                     /locus_tag="OHO85_001249"
     CDS             complement(25615..26682)
                     /gene="rffG"
                     /locus_tag="OHO85_001249"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCQ2670655.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(26682..27944)
                     /gene="wecC"
                     /locus_tag="OHO85_001250"
     CDS             complement(26682..27944)
                     /gene="wecC"
                     /locus_tag="OHO85_001250"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCQ2670656.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(27941..29071)
                     /gene="wecB"
                     /locus_tag="OHO85_001251"
     CDS             complement(27941..29071)
                     /gene="wecB"
                     /locus_tag="OHO85_001251"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCQ2670657.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(29127..30173)
                     /gene="wzzE"
                     /locus_tag="OHO85_001252"
     CDS             complement(29127..30173)
                     /gene="wzzE"
                     /locus_tag="OHO85_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCQ2670658.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(30185..31288)
                     /gene="wecA"
                     /locus_tag="OHO85_001253"
     CDS             complement(30185..31288)
                     /gene="wecA"
                     /locus_tag="OHO85_001253"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCQ2670659.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(31528..32787)
                     /gene="rho"
                     /locus_tag="OHO85_001254"
     CDS             complement(31528..32787)
                     /gene="rho"
                     /locus_tag="OHO85_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCQ2670660.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(32872..32973)
                     /gene="rhoL"
                     /locus_tag="OHO85_001255"
     CDS             complement(32872..32973)
                     /gene="rhoL"
                     /locus_tag="OHO85_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCQ2670661.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(33114..33443)
                     /gene="trxA"
                     /locus_tag="OHO85_001256"
     CDS             complement(33114..33443)
                     /gene="trxA"
                     /locus_tag="OHO85_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCQ2670662.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            33574..34839
                     /gene="rhlB"
                     /locus_tag="OHO85_001257"
     CDS             33574..34839
                     /gene="rhlB"
                     /locus_tag="OHO85_001257"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCQ2670663.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            34973..36457
                     /gene="gppA"
                     /locus_tag="OHO85_001258"
     CDS             34973..36457
                     /gene="gppA"
                     /locus_tag="OHO85_001258"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCQ2670664.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEVMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWYLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(36504..38525)
                     /gene="rep"
                     /locus_tag="OHO85_001259"
     CDS             complement(36504..38525)
                     /gene="rep"
                     /locus_tag="OHO85_001259"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCQ2670665.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEERGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            38742..39191
                     /locus_tag="OHO85_001260"
     CDS             38742..39191
                     /locus_tag="OHO85_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCQ2670666.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVIPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            39390..39671
                     /gene="ppiC"
                     /locus_tag="OHO85_001261"
     CDS             39390..39671
                     /gene="ppiC"
                     /locus_tag="OHO85_001261"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCQ2670667.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(39758..41233)
                     /gene="ilvC"
                     /locus_tag="OHO85_001262"
     CDS             complement(39758..41233)
                     /gene="ilvC"
                     /locus_tag="OHO85_001262"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCQ2670668.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            41383..42276
                     /gene="ilvY"
                     /locus_tag="OHO85_001263"
     CDS             41383..42276
                     /gene="ilvY"
                     /locus_tag="OHO85_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCQ2670669.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(42328..43872)
                     /gene="ilvA"
                     /locus_tag="OHO85_001264"
     CDS             complement(42328..43872)
                     /gene="ilvA"
                     /locus_tag="OHO85_001264"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCQ2670670.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(43875..45725)
                     /gene="ilvD"
                     /locus_tag="OHO85_001265"
     CDS             complement(43875..45725)
                     /gene="ilvD"
                     /locus_tag="OHO85_001265"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCQ2670671.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(45790..46719)
                     /gene="ilvE"
                     /locus_tag="OHO85_001266"
     CDS             complement(45790..46719)
                     /gene="ilvE"
                     /locus_tag="OHO85_001266"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCQ2670672.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(46739..47002)
                     /gene="ilvM"
                     /locus_tag="OHO85_001267"
     CDS             complement(46739..47002)
                     /gene="ilvM"
                     /locus_tag="OHO85_001267"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCQ2670673.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(46999..48645)
                     /locus_tag="OHO85_001268"
     CDS             complement(46999..48645)
                     /locus_tag="OHO85_001268"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCQ2670674.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQLHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGDSNSEMLEKLS"
     gene            complement(48648..48698)
                     /locus_tag="OHO85_001269"
     CDS             complement(48648..48698)
                     /locus_tag="OHO85_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670675.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(48785..48883)
                     /gene="ilvL"
                     /locus_tag="OHO85_001270"
     CDS             complement(48785..48883)
                     /gene="ilvL"
                     /locus_tag="OHO85_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCQ2670676.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            49236..50756
                     /locus_tag="OHO85_001271"
     CDS             49236..50756
                     /locus_tag="OHO85_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HCQ2670677.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(50781..51119)
                     /gene="maoP"
                     /locus_tag="OHO85_001272"
     CDS             complement(50781..51119)
                     /gene="maoP"
                     /locus_tag="OHO85_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCQ2670678.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            51238..52077
                     /gene="hdfR"
                     /locus_tag="OHO85_001273"
     CDS             51238..52077
                     /gene="hdfR"
                     /locus_tag="OHO85_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCQ2670679.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(52173..52248)
                     /locus_tag="OHO85_001274"
     tRNA            complement(52173..52248)
                     /locus_tag="OHO85_001274"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52213..52215),aa:Trp,seq:cca)
     gene            complement(52257..52333)
                     /locus_tag="OHO85_001275"
     tRNA            complement(52257..52333)
                     /locus_tag="OHO85_001275"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(52297..52299),aa:Asp,seq:gtc)
     gene            complement(52388..52503)
                     /gene="rrf"
                     /locus_tag="OHO85_001276"
     rRNA            complement(52388..52503)
                     /gene="rrf"
                     /locus_tag="OHO85_001276"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        12782 a        14329 c        13364 g        12102 t
ORIGIN      
        1 gcagaaggtg gcgttaaatc tcgcttcagg tgaagggaat ttctttatcc gtcctggcgg
       61 cgtgttttat gtcgcgggag ataaagtcgg catcgttcgt ctggatgcct ttaaagccag
      121 taaagagatt cagtttgcgg tgcagtcagg gccaatgttg atggaaaacg gtgtaattaa
      181 tccgcgtatt catcccaacg tcgcctcacg taaaattcgt aacggtgttg ggattaataa
      241 acatgggaat gccgtgtttt tgttgagcca gcaggcaaca aatttttatg atttttcctg
      301 ttatgccaaa gcgaaactga acgttgagca gctgctttat cttgatggca cgatttcaca
      361 tatgtatatg aaaggcgggg caatcccgtg gcaacgctat ccttttgtca ccatgatttc
      421 cgtcgagcgg aaaggttaga atgatagttt tgtgtcagat actaaatatg ctgctgatgc
      481 cggatgcggc gtaaacgcct tatccggcct acatgacgtt gcaatttatt gaatctgaat
      541 gattttttag gccaaataag gtgcgcagca ccgcatccgg caacgttaca ccgtctccag
      601 ccgggcgtaa gccgccacca gccatttgat tccctggccc tggaacgcca cctgcaaacg
      661 gctatgttca ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg
      721 tacacgctga ccgagcttgt agccgctgtc gttctcgacc atcggcgtcc ccatccgttg
      781 atggctgacc ggacgactta ccgtagcgcg caggcgcacc tcttccacac actcttccgg
      841 cagctcgccg ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt
      901 ttccgcgtag gtcaacgtca gtttctgcat cgcgcgggtt acgccaacgt aggccagacg
      961 gcgttcttct tccagacgcc cgccttcatc cagcgacatc tggcttggga acatgccctc
     1021 ttccataccg acaataaaca cctgcggaaa ctccagacct ttcgccgagt gtagcgtcat
     1081 caactgcacc gcgtcctgcc aggtatccgc ctgcccttcg cccgcttcca gcgccgcatg
     1141 ggagagaaac gcctgcaacg gcattaaatc ttcatcttct tcgttgtagc tgaactggcg
     1201 cgttgccgtc accagttcct ccaagttttc gatacgcgtc tgaccttttt cgcccttctc
     1261 ctgctcgtac atggtgcgca ggccggagtc tttaatgacc cggtcagtct gtacatgtag
     1321 cggcatatcg gcagtttctt gcgctaaggc gtcgatcagt tccataaacc gctgcaaggc
     1381 gctggctgca cgtccggcga gcgctttttc ctgcaacagt tcacggcacg cctgccacag
     1441 cgttaactgg cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc
     1501 ccgcgttggt gtattcacta cgcgctcaaa ggccgcgtcg tcgttgcggt tggcaatcag
     1561 gcgcagatac gagagcgcat ctttgatttc ctggcgttcg aagaagcgca tcccgccgta
     1621 aatacggtac ggcatactcg cctgcaacag tgcttcttcc agcacgcgcg actgggcgtt
     1681 actgcggtag agaatggcgc actcggcaag cgctccgccg ttatcctgcc aggttttgat
     1741 gcggttaacc acaaaacgcg cttcgtcgag ttcgttaaag gcgcaataaa gagaaatcgg
     1801 ctcaccgtcc gcgccatcgg tccacaattt tttacctaag cgcccattgt tattttcaat
     1861 cagggcgtta gccgcgctca gaatattgct ggtagagcgg tagttttgct ccagacgaat
     1921 agtttcggca ccggggaaat cattaaggaa acgctgaata ttctctacct gcgccccgcg
     1981 ccagccgtag attgactggt cgtcatcgcc aacgatcatc actttgccgg tatcgcccgc
     2041 cagcaggcgg atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat
     2101 gttggtaaaa cgttcgcggt agtgttgcag gatatgcggc ttgttaagcc acaactcgtg
     2161 agcgcgcagc agcagctcgg cgaagtccac caggcccgca cgatcgcacg cttcctgata
     2221 cgcctgatac accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg
     2281 cggacgcagg ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca
     2341 ctgcttctcg tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc
     2401 actgtcgagg atctggaaat cctgcggtag attggcgtcc atatggtgcg cacgcagcaa
     2461 acggtgcgcc agcccgtgga aggtgccgac ccacatgccg ccctggctgg tgcccatcag
     2521 ttgcccgata cgatgacgca tttccgccgc cgctttgttg gtaaacgtca ccgccataat
     2581 cgagtatggc gagcagtttt ccacgctcat caaccaggcg atacgatgca ccagtacgcg
     2641 cgtcttacca ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc
     2701 ttcgcgctgt ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc
     2761 gtaaaaatag gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac
     2821 agatttgctg attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgcggc
     2881 agtaaacggc tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc
     2941 tgcattccgc tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag
     3001 atctcgccga tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt
     3061 tttgagcgcc cgtgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac
     3121 agctccggct gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt
     3181 tttaaggtgt cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagttg
     3241 atcattgctg cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc
     3301 gcttgttcaa tcgaacgaaa acgccagcgc gtcacgtcgt gataaatctc gggttccgct
     3361 tcccgtaccg cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga
     3421 tgataatttt gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta
     3481 tcgtaaaggg tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa
     3541 aaacgcatta tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt
     3601 gttgaaaatc aagatgagta tagatttgcg tggtggagag gttggcatga cccagcagct
     3661 cctgcacacc acgaagatcg ccgctcgact ccagcatatg cgtagcgaac gagtgacgta
     3721 atttatgcgg gtgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt
     3781 tctgcacatt acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt
     3841 cgctaccaaa caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac
     3901 caatcggcag gcggcgctct ttgctgcctt tccccattac ccacacttca ccagactcca
     3961 ggtcgaggtg tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca
     4021 tcacttccag cattgcacgg tcgcgtacag cgaggggatc gttgatatca atatccagca
     4081 gccgattcat atcgtcgacg tcgatgtttt tcggcagatg acgcggcgct ttcggtgccg
     4141 aaactccttt cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc
     4201 tacgtagcgc agaaagccgt aacgccagac ttgctgctcc cagcccttta cggcgactgc
     4261 gtacagcaaa attgcgtacc atcgctgcat cgcattgttg ccagctttgc aggccgtttt
     4321 cgctggcaaa attgatgatc gcctcaagct gacgctggta gttaagtagg gttatcgggc
     4381 taagctgacg ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg
     4441 tcatacgcgt tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag
     4501 taactgcgtt ccctgccctt gttgatagtg actggcatca cgactggtaa acagcacgac
     4561 acccaaatca gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc
     4621 cggtagcacc accagcagct ctggtccgtt aagcggccca agatagtgct gttcctgccc
     4681 caaacgctga atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt
     4741 cgacggcgca cctaagcgcc agcgatccgg aaacaggcgc agactcgcac ctgccaggcc
     4801 gagatcgcgc gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt
     4861 gagactgcgc tgcaggtaga gtaggcgata aaacaggcct tcgttggcga tagcctgttc
     4921 catcaacagc accatgttct cttccagaac atgaatatga ttacgtgcgc gggccatgtg
     4981 ccactcgacc aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc
     5041 gcgcgcattg cggataaaaa actcaggatt tttaatcaga taatcgacaa ccgcccggtc
     5101 atcaagctcc gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat
     5161 ccgtcgtaga catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag
     5221 gcgatatcaa gacgaccgcc ggggagttcc acgcgtactt cttcggccag caaaccttgc
     5281 tgaatcccta ctgcaaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc
     5341 ccacgctcat aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata
     5401 ttggctcgct ccggaaaacg ctcgtggctt tccagaacag gaccaagcgt ttctaccgcc
     5461 gcggtatcga catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg
     5521 cataagattg tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga
     5581 aacggcacag cggaaggttc gaagttgggt tcgcccatat ttacgcggac caaatcatca
     5641 tcggtgacgg tcagaaccat ccgcccgttg gcggtgctga cgcggatatc gcgcttattg
     5701 gtaagccctt tcaggcgcac aaaacgggca aagcagcgcg caccgttgcc gcactgcgcc
     5761 acttcactgc catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc
     5821 ggctcaacca ccagcagctg gtcaaaccct acccccaggt gccgatccgc caggcgacga
     5881 atcagctccg gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg
     5941 ccaaggccat gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact
     6001 gacttttagt aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc
     6061 accgtggatt gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcatt tttatctgca
     6121 ggcgggaaat agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat
     6181 acagtgagtg ccttaaacac gtttttcatt atggattgcc tgtaaagttc atcgctttct
     6241 gctttctatc atcgcaggag aaggcgtaaa agcaagcatt tagcgactct ttatgaagtc
     6301 tgaaaagtga ggaaatgctg cgagtataac gcttactcgt ggggatttat gttctgctgg
     6361 cagtttcgtg atcaacacct cgcgccagga tggctttatg cactatcgca atgtggtttg
     6421 tttgttggct tttttagttc cgtcagcgtg aggatgctcg ctcggtaaac ctgatcataa
     6481 tgtttaccag aaacagggaa aggcgtggtg taactgcgcc ctaatgaaga gacaaatctt
     6541 tcgctacact gagacatcat tattccggtg acggatgaat cgtgccggaa cgcagccgca
     6601 gaggtgcaaa ctttgaagcc ctgaccccat gccgctatac tgccgccatc acttaaaaac
     6661 aggatacaac aatgaacgac agtgaatttc atcgcctggc tgatcaactc tggctgacca
     6721 ttgaagaacg cctggacgac tgggatggcg acagcgatat cgactgcgaa atcaacggcg
     6781 gcgtactgac cattaccttt gaaaacggca gcaaaatcat tatcaaccga caggaaccgc
     6841 tgcaccaggt atggctggca accaaacaag gcggctacca ttttgatctg aaaggcgatg
     6901 agtggatttg cgatcgcagc ggcgaaacct tctgggattt gctggaacag gcggcgacgc
     6961 agcaggcggg tgaaacagtc agtttccgct aagattgcat gccggataag cctcgctttc
     7021 cggcacgttc atcacgaaaa atactgctgt aatagcggcg tatcgtgatc ctgattggca
     7081 ggcggcatgt taccgataga ttttgtgcgg aacggaatca cctgttcacg accatcaacc
     7141 ttcacaatct gatagaactg cggcaggttg aagttgatga agcttgagcc gtaggtaaaa
     7201 cggtcatgcg atgacgagta gaagcgactg acgtcacgta ccagctcctc tttgctgcct
     7261 tcgcagtggt gatacacctc aacccggttg ctttcgtcga gaatgtagat attaaagcca
     7321 ttctcgtctt gcgtttcttc gaaaaagaac tggatgatcc cttcgctggc aaagccgtcc
     7381 accaccgccg gtaatttgac gtgattggtt tcaacctgca ctgacaggcc gtgcagtttg
     7441 ttatgcgaaa tcgcgccata aaactcgatg gcgttttcca gtttctgtac cgatacattc
     7501 aggcgttcga agaacaaccc ccaggtttga ccagaaacgc gcagcgcctt gaaacgcccg
     7561 gtttcctggc gggtgctgga aagacgcaat tcaatacact cagaaaccag ttgctgcacg
     7621 cgagtacgaa ttaagccgcg cagatgctgg ctataacaga agacttccac gctatctggc
     7681 ggtgcggcgt cctgatgcat tttgccgaga atagttttca gggcttcgat catcgattgc
     7741 tcgccgttga aatgcagcgt acgcacttcg ttccacgagt tgcggtacag caggtcaacg
     7801 ctacctacca ggcaattttg attctcgcca aagctgaaga catccagctt acggaaatcg
     7861 aaatgcacca cctgattgcg gaacgccgct gtcgggtcat attccaggtt aacgataatc
     7921 gccagatggc ggatctcaca cgggctgtag agcgccttcg gtgtcggtgc aggtaagcgc
     7981 agcgggaaat ggtgcgacac gtcggcgacc atctcctgca acttaggcaa atcgacaatg
     8041 ccgttacctt taatatacaa acgggtgcgc gaggtcagca ggccgttaaa ccatgcccac
     8101 gccaccagtt tattcaggta acggttatat tccagcggct gatggctgat gatcgactca
     8161 atatttggcg cgcggttata cagataccaa cctgaacggt tagcacggcc cggcggcaca
     8221 taaataaagg tcagattcgg ttccgagaga tcgggtgaaa tctgcgggtt taccagcgtc
     8281 actttacctg gtaatgcttc aaacgcggca tacagcttac gcgtcagcac gccgatatcc
     8341 tgcggactgg cggagacgct aaggttattg cgacgcgcaa agcggatcag attacggtag
     8401 ctctgcatca tcgcgtcgag caactcgttg tgcgcctcac gcacctgatc aatcttccag
     8461 ttagcgcggt tatcgagcat tgccagacga gcttcgtccc aaccccactc gctcactaac
     8521 tggctcaaca ctgcgcgacg ccagcctacg caggcgcgtt cacggctgag cttttcgcac
     8581 acttttaaat agaagcagcg acgtactaaa tccagacggg taaaatcttc aatcgccgtc
     8641 aggtattcag taacacgctc cagcatcatg cagtatggat cgagaccaaa cgatacaatc
     8701 tcgccgtcgt gcaaacgctg tttgatatct ttcgccagca gacgtgggtt cgggtattcc
     8761 caggaatagg cttccagcag cagtgttttc agcaccgctt tgtatgggga atcgatactc
     8821 ttgtagagct gccaaaggct ggcaccaaag tactcttcag cagaaagcga gcttaagcca
     8881 ccgagatcca gccattcatt tggcgtcagc acgccctgcg cgtaaagcgt catcacatag
     8941 tcgtcgtaat gttcttcttc gtcgcacggc accatattcc acagaatacg cttaccggcg
     9001 agacgcacgg cggtacgata aaattcgtca agcagcagta tatgctgggt ggagccacaa
     9061 tcttcgcccc ccaggctgcc gctttcatta tgacggaagc ggttttcatc aatcaggaag
     9121 aagctgactt ccacacccag cgaggcggcc cagctttcca gcagactaca tttacgttgt
     9181 agcaactggc gctcttcgct atcgagccag gattgatgac agacccagat atccaggtca
     9241 gaggaacaac tttgccctac ggacgaggtg ctgcccatgg tgtatacacc agtaattgga
     9301 agctcacctt tcggcgggtc ctgtactgac attccacgat acagttcaag ctcgttcagg
     9361 tagtggcgtt gagtttcatc aggcgtgtaa aggcaaatgc ctttgggaac gttaccatca
     9421 aggtaacccg gcattagcgg atggtgatag tgcaacaatg tcggcagtag actgtagacc
     9481 tgttggaatg caggccccat agcagcaagc gcgcgatcca cacgcaattg atttatggca
     9541 tccagtctct gtttcagagt ctcaatatag aggtacaaga cgtatcgcct gatttgctac
     9601 ccgtcatgac tgtgattccg ccaacatcaa cggtaacacg cggcattcgg gatatttcgt
     9661 atgtcaaagg taaccgttac cacttttcgc gcctggtttt tttagtttca cgacgaaaaa
     9721 atggtctaaa acgtgatcaa tttaacacct tgctgattga ccgtaaagaa agatgcgcta
     9781 catacaagtg tagcaccgtt tattctctgt aaattcctta ttacgacggc gtgaaacgcc
     9841 tgtcaggatc cactgccaga cctcatttta cggtttgcgc aggcgtctac gtttcaccac
     9901 aacactgaca tcactctggc aaggatgtta ggatgaacca cggatgataa tgacggtaac
     9961 aagcatgtta gacaatgttt taagaattgc cacacgccaa agcccacttg cactctggca
    10021 ggcacactat gtcaaagaca agttgatggc gagccatccg ggcctggtcg ttgaactggt
    10081 accgatggtg acgcgcggcg atgtgattct tgatacgccg ctggcgaaag taggtggaaa
    10141 aggcttattt gtaaaagagc tggaagtcgc gctcctcgaa aatcgcgccg atatcgccgt
    10201 acactcaatg aaagatgtgc cggttgaatt tccgcaaggt ctgggactgg tcactatttg
    10261 tgagcgtgaa gatcctcgcg atgcctttgt gtccaataac tatgacagtc tggatgcgtt
    10321 accggcaggc agtatcgtcg ggacgtccag tttacgtcgc cagtgccaac tggctgaacg
    10381 ccgcccggat ctgattatcc gctccctgcg cggcaacgtc ggcactcgcc tgagtaaact
    10441 ggataacggc gaatacgatg ccatcattct tgccgtagcc ggactaaaac gtttaggtct
    10501 ggagtcccgc attcgcgccg cgttaccgcc cgagatttct cttccggcgg tagggcaagg
    10561 tgcggtgggt attgaatgcc gccttgatga tgcacgcact cgcgagctgc ttgccgcgct
    10621 gaatcaccac gaaactgcac tgcgcgttac cgcagaacgc gccatgaata cccgtctcga
    10681 aggcggatgt caggtgccaa ttggtagcta cgccgagctt attgatggcg aaatctggct
    10741 acgtgcgctg gtcggcgcgc cggacggttc gcagattatt cgcggtgaac gccgcggtgc
    10801 gccgcaagat gccgaacaaa tggggatttc gctggcagaa gagctactga ataacggcgc
    10861 gcgcgagatc ctcgctgaag tctataacgg agacgccccg gcatgagtat cctggtcacc
    10921 cgcccgtctc ccgctggaga agagttagtg agccgtctgc gcacactggg acaggtggcc
    10981 tggcattttc cgctgattga gttttctccg ggtcgacaat taccgcaact tgctgatcaa
    11041 ctggcagcgc tgggggagag cgatctgttg tttgccctct cgcaacacgc ggttgctttt
    11101 gcccaatcac agctacatca gcaagatcgt aaatggcccc gactacctaa ttatttcgcc
    11161 attggacgca ccaccgcact ggcactacat accgtaagcg gacagaagat tctctacccg
    11221 caggatcggg aaatcagcga agtcttgcta caattacctg aattacaaaa tattgcgggc
    11281 aaacgtgcgc tgatattacg tggcaatggc ggtcgtgagc taattgggga taccctgacg
    11341 gcgcgcggtg ctgaggtcac tttttgtgaa tgttatcaac gatgcgcaat ccattacgat
    11401 ggtgcagaag aagcgatgcg ctggcaatcc cgcgaggtga cgacggtcgt tgttaccagc
    11461 ggtgaaatgt tgcagcaact ctggtcgctg atcccacaat ggtatcgtga gcactggtta
    11521 ctacactgtc gactattggt cgtcagtgag cgtttggcga aactcgcccg ggaactgggc
    11581 tggcaagaca ttaaggtcgc cgataacgct gacaacgatg cgcttttacg ggcattacaa
    11641 taactctcat aacaggaagc cataatgacg gaacaagaaa aaacctccgc cgtggttgaa
    11701 gagaccaggg aggccgtgga caccacgtca caacctgtcg caacagaaaa aaagagtaag
    11761 aacaataccg cattgattct cagcgcggtg gctatcgcta ttgctctggc ggcgggcgtc
    11821 ggtttgtatg gctggggtaa acaacaggcc gtcaatcaga ccgccaccag cgatgccctg
    11881 gctaaccaac tgacggcatt gcaaaaagcc caggagagcc aaaaagccga gctggaaggc
    11941 attattaagc aacaagctgc acagcttaag caggcgaatc gtcagcaaga aacgctggca
    12001 aaacagttgg atgaagtcca acaaaaggtc gccaccattt ccggcagcga tgctaaaacc
    12061 tggctgctgg ctcaggccga ttttctggtg aaactcgccg gacggaagct gtggagcgat
    12121 caagacgtca cgaccgctgc agcgttgctg aaaagtgcag atgccagcct ggcggatatg
    12181 aatgacccga gtctgattac cgttcgccgg gcaattaccg atgatatcgc cagcctttct
    12241 gcggtatcgc aggtggatta tgacggcatc atccttaagc tcaatcagct ttcaaatcag
    12301 gtagataacc tgcgtctggc cgataatgac agcgatggtt cgccgatgga ttccgacggt
    12361 gaagagcttt ccagttccat cagcgaatgg cgtatcaatc tgcaaaaaag ctggcagaat
    12421 tttatggaca acttcattac gattcgccgt cgtgatgaca ccgccgtacc gctgttagcg
    12481 ccaaatcagg atatctatct gcgcgaaaat attcgctctc gcctgctggt cgcagcacaa
    12541 gctgtaccgc gtcaccagca agagacttat cgccaggcgc tggagaacgt ctccacctgg
    12601 gtacgtgctt actacgatac tgatgatgcc accaccaaag cgttcctcga cgaggtggac
    12661 cagttaagcc agcaaaatat ctcgatggat cttccggaaa ccctgcaaag ccaggcgatg
    12721 ctggaaaaac tgatgcagac tcgcgtgcgt aacctgctgg cacaaccggc agcggggaca
    12781 acggaagcta aacctgcacc tgcacctgca cctgcacctg cacctgcacc tgcacctgca
    12841 cctgcaccgc aagctgatac ttcggcagct gcgccgcaag gagaataacc atgctaaaag
    12901 tgttattgct ctttgtgttg ctgattgcgg ggatcgtggt tggcccgatg attgccggtc
    12961 atcagggtta tgtgctgatc cagaccgaca actacaatat cgaaaccagc gtcacgggcc
    13021 tggcgattat tttgattctg gcgatggtag tgctgtttgc cattgagtgg ctactgcggc
    13081 ggatcttccg cactggcgcg cacacccgtg ggtggtttgt cggacgtaag cgtcgccgtg
    13141 cccgtaagca gaccgaacag gcgctgctga aactggcgga aggcgattat cagcaagttg
    13201 aaaagctgat ggcgaaaaat gccgatcacg cggaacaacc ggtggtgaac tatctactgg
    13261 ctgccgaagc cgcgcaacaa cgtggtgatg aagcacgcgc caaccaacat ctggaacgcg
    13321 cagcggagct ggccggcaat gacaccattc cggtagaaat cacccgtgta cgtctgcaac
    13381 tggcccgtaa tgaaaaccat gctgcacgcc acggcgtgga taagctgctg gaagttacgc
    13441 cacgccatcc ggaagtatta cgtctggcgg aacaggcgta tatccgcaca ggtgcatgga
    13501 gttcgctgct ggatattatc ccatcaatgg cgaaagccca tgttggtgat gaagaacatc
    13561 gcgcaatgct ggaacaacag gcatggattg gcctgatgga tcaggctcgt gccgataacg
    13621 gtagcgaagg tttgcgtaac tggtggaaaa accaaagccg gaaaacgcgt catcaggtag
    13681 cgttgcaggt ggcaatggcg gaacatctta ttgaatgtga cgatcatgat actgcccagc
    13741 aaattatcat cgacggcctg aaacgccagt atgacgatcg cctactgctg ccgatcccgc
    13801 gtctgaaaac caataacccg gaacagctgg aaaaagtgct acgccagcag atcaaaaacg
    13861 tcggcgatcg cccgctgttg tggagcacac tgggccagtc actgatgaag cacggagaat
    13921 ggcaggaagc atcgctcgcc ttccgcgcgg cgctgaaaca acgtccggac gcctacgatt
    13981 acgcatggct tgccgacgcg ctggacagac tgcacaagcc ggaagaagct gcagcgatgc
    14041 gtcgcgacgg tttgatgtta acgttgcaga acaacccacc acagtagttc cttctcaccc
    14101 ggaggcaagc acctccggga cctttctgat gcataaaaaa acgcctgctc ttattacgga
    14161 gcaggcgtta aaacaggtct gtatgacaac aagtgggtgc ttcactcaac gttgtgtcca
    14221 tggtgtctga tgaggcataa gcgacatctg tcagtggacg ataagcaccg taaacggctc
    14281 tgcgtcattc cggagtttat gaggcactaa ggcgaacata agagatggaa tgagcatcta
    14341 cccgtttatt atgccacaga gaatcgggaa ataacatccc ttaacacttg ttatgagata
    14401 attctgtaat cctctttgct tcctgagtaa taacttcctg agtgaatatt taacctgagc
    14461 ttgatcctac acatacttat tatgaatgat aaaattcatt caattaataa cacatatatt
    14521 aattgccgtt aaaactaaaa aaagcatcaa taatcaacgc gatataataa acctgcctta
    14581 catatcaact gcgccagagg taggattgaa aacgctctcc tgattttcca attcattttc
    14641 tggataaata aataatttat ttttgtcact attatttatg taatcatcct gtcagggaga
    14701 gggatctcaa ttatcaatgc ttaattacgt catcattttg atgacatgaa accggagcat
    14761 tttgcatgga attttcgttt tcacccaaac ttcttgttgt tgctgtcgcc gccgctcttc
    14821 ctctcatggc cagcgcagca gataccccgt caactgccac cgcacgcaaa ggctttgccg
    14881 gatacgatca cccaaaccag tatccacaga acgctgcaat ttattgaatt tgcacgatca
    14941 tgtaggccgg ataaggcgtt tacgccgcat ccggcaatca accgcaggcg gccacaaaat
    15001 ggccgctttt ttacttactc accaccagca aatgtgcctg cataatgtca ctggccgggc
    15061 gaccgtgcgc cagcaaatca gccattgctt taagatatgg cggtagatgg cggaaataac
    15121 gctgataccc ggcacacaaa taattcagcc ccggtttgcc gctggcatcg agcatgaagc
    15181 ggtgtttcgg gcagcctccc cagcacgctt ttaacacgtt acaactgcga cactgcgccg
    15241 gtaattgctt aaatttatct tcaccaaaca cctgctgttg cggggaatcg atcatttctg
    15301 caattgtttg ctgatgcata ttccccagcc gatattgcgg ataaacatag tgatcgcagg
    15361 cgtaaacgtc gccgttgtgc tcaacaatca ccgagcgccc acaggttggc tgatgatggc
    15421 aaaccgcacc cggcgcaccg acaaaattgg caaacgccca ttcgatattc atcacgaaaa
    15481 tcttgccgac gtcgcgtttg atccagtggt cgaatatcgc caccagaaat tcaccaaatt
    15541 tatcggggcg caccgaccat tccgtcagtt ccccctgaat atcaccaggc gcatgcaatt
    15601 tcagtccatc gcgggcagct gtttcatcag ccagacgctc gaccaccgga ataaactgga
    15661 tgaattcgac tcccgcatcg cacaaaaaat cataaacctg caacggttgc tgcgcgctgg
    15721 tgcggttgac gcagaccagc acgttatagt cgacatgatg tttttgcagc agcgtcaggg
    15781 cacgcatcac cagcttatgc gttggtctgc cacctttggt cacgcgatat tgattgtgga
    15841 tctcagccgg accgtccagc gataacccaa caagaaaatg attttctgcc agaaatgcac
    15901 accatttatc atcgagcagc acgccgttag tctggaagct gttacttatc ttcctgccag
    15961 caccgtattt cgcctgtaac tttacggcac ggcggtaaaa atccagcccg agtaacgttg
    16021 gttcgccgcc ctgccaggta aaagccactt cgttttgcgg ttcgctggca gcgatatagt
    16081 gacggacata cgcttccagc gtgtcatcgt ccatatgcgt gactggcttt tcgcggtaaa
    16141 gggattgttt ttcgagataa aaacagtagt cacagttcag attacaatcg gaaccactcg
    16201 gtttcgccat cacatgaaaa gcacgcgttg gaacctgttg cagcatggtc actccttgcc
    16261 tgaagtcatc cttcaatacg ttaagggcga aatcataccg tggggcaatg agttattacg
    16321 ggcgtttatt gcgaagggag ggtgacgaaa tgcacagaaa acaaaaaacc ccgccgaagc
    16381 ggggttcaaa attggtcggc gagagaggat tcgaacctcc gacccactgg tcccaaacca
    16441 gttgcgctac caagctgcgc tactcgccga aatactgctt tttgaatttt tagttcaatt
    16501 ctttaaagtc gtggtgcgag gggggggact tgaaccccca cgtccgtaag gacactaaca
    16561 cctgaagcta gcgcgtctac caattccgcc accttcgcat tgtcacaact tctaataatg
    16621 gggtggctaa tgggattcga acccacgaca actggaatca caatccaggg ctctaccaac
    16681 tgagctatag ccaccactac aaatcttgtt acgcggtatt actaccaccg cagctcaagc
    16741 gccgggacta aatggcgcgc ccgacaggat tcgaacctga gacctctgcc tccggagggc
    16801 agcgctctat ccagctgagc tacgggcgct tagcgccgtt gcgggggtgg ataatacgga
    16861 cttcccaccc ctctgtctag tcccttttta aataaattgc gcgtttggtt acggtttgtg
    16921 cgttttgctg cttattcctc cagtttatgc gctttcccgt ggcgattaag gccaaaaact
    16981 ttataaatcg ccgtcaccgc cagcataaag atgatgccaa caaacagcga catacgcgta
    17041 tcttcattaa agtacatgcc gatcaaaacg caaatcagga atgccattgt tacgtagttg
    17101 gcccaaggga acaggattga gcggaacgga tggctggcaa tcgccgcttt atgtgcacgc
    17161 cgaaaacgca gctggcttat caatatcaca aaccatggca ccatccccgg aagcacgctg
    17221 gcactgtaga cgtagacaaa cacacgctgc ggattgggaa tgatgtagtt caggcatgag
    17281 ccaattagca gaatagcaat agatactgcc acacccgcaa ctggtacgcc gtgacgggaa
    17341 actttcgcca tcgccgccgg taactgacgg tttttcgcca gtgcgtagag catacgtccg
    17401 caactgtaca tgccgctgtt acagccagag agcgcagccg tcagcaccac aaagttgata
    17461 atgcccgccg ctgcggtaat accgattttg gcaaaagtca gtacgaacgg gctgccgttg
    17521 ctgccaattt cattccacgg gaagatggtg acgataacga aaatcgcgcc tacgtagaaa
    17581 atcaggatcc gccacagcac tttgcctacg gcactgcgca gcgtcacctg cggattcttc
    17641 gcttcaccgg cagtaatgcc aatcagctcc acgccctggt aggacgccac cacaatacac
    17701 agcgcggtca ggaacccttt ccagccaccc gcaaagaaac cgccatgctc tgtgagattg
    17761 ctaaaaccaa tcgactgccc gccattgcca aagccaaaga aaatcacgcc caggccaatg
    17821 acaatcatca cgataatcgt ggtgactttg atcatcgcga accagaactc gatttcgccg
    17881 tacaaccgca ccgccgccag attcgccaac gccaccagcg ccactgcgat caatgcgggt
    17941 atccactgcg ccatctccgg gaaccagaac tggacataaa cgccaatggc ggtgatttct
    18001 gagatcccca ccgccatcca cataaaccaa taagaccagg cggtgagata accaaagaac
    18061 gggctcatat aacgatgcgc ataaacggcg aacgaaccgg taaccggttc gaggaacaac
    18121 atttcgccca ttgaacgcat gatgaaaaag acgaatagcc cggcgatgat ataggccaac
    18181 aatacggatg gcccggccca tttcaaggta ctggcggccc ccataaacag gccgacgcca
    18241 atggtgcccc ccagggcgat gagttcgata tgtcgagctt ccagcccacg ctgtagctct
    18301 ggtttgttat ctgccataaa tcctcgtgtt gtgtttgcat gctttccggt gttaccggtt
    18361 atcgttatgg gtacatcgag tgttgcgaat gttttcgtaa ttcaggagaa atggcaaata
    18421 aagcattaaa aatttgaatg ctttgtgtaa taaaaaagca gacaggcgac gaagtgacca
    18481 ctccgtcgct ttacaaagag aggaaaatca gaggttgccg gtgtaatgcc agcgtaaata
    18541 acgcagcaaa cgaagctgac gcttaatgcg gctcggctgc gaaagcaggc ggtagagcca
    18601 ctccagtccc agcgtttgcc agattttcgg tgcgcgtttt acgtgaccgg tgaaaacatc
    18661 gtaagtcccg ccaacgccca tatacagcgc atctggatgc accagacggc agtcgcgcat
    18721 gaagatctcc tgctttggcg atcccatcgc aacggtgacg atttgcgctc cgctggcatg
    18781 aatgcgttca aacagcgcct gacgctgctc gggtttaaaa taaccatcct gactgccaac
    18841 gatattcaca ttccattggt tgcgcagttt agcttcagtt tgcgccagca cttcaggttt
    18901 accgcccaca agaaataccg gcgtcccttc tttgcctgcg cgcgccatca gctcttccca
    18961 gagatcggca ccggcaacgc gggaaacctg cgcctgcggg tactttttac gtactgaacg
    19021 tacaacgctg atgccatccg catatttaaa ttcggcagcg ttaattaact ccctgacctc
    19081 ggcgttatct tcaatggtca gcattttttc agcattaatg gcaaccagcg ttccctgctt
    19141 aagctgcccg tcagcaaaca gataatcgag ggcgtgctgc atatcacgcc aaccaatcaa
    19201 ctgtaagcca cgcagcgtat aggtcggtgc cgtggtgttg ttattcattg ttatccttca
    19261 acctgcgtcc ggagcgatga ttttgtacgt ttatgaatga gtccggcgct ttcaaaaagc
    19321 cagtacaaca gttttgcgat catcagacat gcgccgaaga ccacgataaa aaagactacg
    19381 cgtgagacaa acgaatccag cccttcacgt gccagcacga tcatattgaa gatcgcccca
    19441 aagcagaaac tgtgcaatat cgcagcctta tagcgattag tctcgcggtt gcccagctca
    19501 tacagccagt cgaaccattt gatgatcagt ccaaccacaa tcgccccgag cgggatgaac
    19561 aacgcgccgc ccatcaccac cagtgagcct ataagcgtag gcgagatcgc cagtccggag
    19621 tggttattca gcacttccca ggtaaagtag ttggctgagt tcagcaccat actcgggcgc
    19681 cccggccaca gccaggaagg gataaagaca tagaaatcgc ggacaattgg agccaggccc
    19741 tggaagtcga tgttgtcgta gttctgcaac agcaacgcca gattctccca cggcgagaag
    19801 gtgtcgcgag tgagatagag aaacgtatag aacgcttcat cgccgctcac attcattcca
    19861 tagcgtttta acgccagcca gaacatgcca acaatcccca gcacgcccgc cgccgccagc
    19921 atccacaacg aaatccagcc gcgaataatg ccaataaaca ggaagatagc gaatgcgatg
    19981 atgatattgg cgcgagtgcc gccgacaatc atataagtca gcaagccaaa agcgaccgtg
    20041 ctgacgagga aaaacagcca cgctttgctg tcctggcgca gaaagtagac caccagcatc
    20101 gccgggatga aaaagtaaaa gaagcgtttt aacgccacgc cggagacttc actggagaag
    20161 atctgactgt aggagttaag ccggaacagc aaaaagccgt tgtgcatgaa gaagatgccg
    20221 acgcttacca gcgcgatgcc catcaggatc acccacgtga gattggtctc cacgcggttc
    20281 atggtaaaca gcggacggcg cggtacatca gcaacgcgtt tgcgtaggcg ggttttgtag
    20341 gtgacatagt aaaccgcgta gaagcagccc gcagaaagca acgcctgcaa caagatttct
    20401 ggcggcgcga caccaacatc aaagcgaaat accagcacgc tggtcagcgg gaagccgaag
    20461 aaaaaggtga gcaaaaacag caatgaaaag aagacattga agttaaagcg cacacggcga
    20521 aactcaaacc aggtcagcgt ggcaataaac agcgtgcaga gcagccagac cacaaacagg
    20581 ccactgaatt gcagcagact catgcgacct ccccggcggc aatcgccaac gcccgctgcc
    20641 agccttgtag atagttaggg ctaaagaagg caatggtgtt tttatccacc gacgccaact
    20701 ggcgctgcgc ttcaagcaca atatcctcgt tgagatcgtc ggtagtaaac agcaccggca
    20761 aatgctgttc cgtcatatcc tgccagaacg gattttcccg gttaagcaca caaggaatgc
    20821 ccgcctgaat cagtaagcac agcgtaccaa tgccctgctg gcgggcaaaa ataaagtaac
    20881 cgagatcgca ctgacgaagt agcgccagat aggcgtcaaa ttccagtttt tcgctcagaa
    20941 cttgcagatt ttcttcgctg aataactcca gccccgcctg acgaacttcc tcaatgtacg
    21001 cttcgttatt aggcggatat cccatcggca ccaccacttt taccgtatcg ccaaattgct
    21061 gatgaacggc acgtaaggcg gcgacatgct cattgctgcg gtcgccggag ttccccacca
    21121 gaatggtcat tttcccttca cgttgccgat cgttcgccat cgtattgaga gaagggtcca
    21181 tccgcgtcgg gaagtacagc agttcgcccc gcacctttgg atgcgttttg gcaaaaaagc
    21241 tcaaatcacc gcgggtggca aatacatagc cgactcgctt ttgcgccagg cgacgtagcg
    21301 ggtaaaaaag cttatatctc aagccactgg aaagctcata caggtctgcc ccccagatat
    21361 gccagaaaaa ctggctgggc ttaatgccac cactcagcag agccagccac agtgtgggat
    21421 tgaactgacc gtggaaaaaa aatcgctgct gacggttagc ttttgctttc gcgatgatcg
    21481 cttccgccag cgattttttc ccagggaaaa attgcacaga aagcgccgga cagctatcac
    21541 ttaagccgtc gtccttgcca acaaccataa actcgcgcgc gtgctcgctc gtcgcggcca
    21601 gcgcgtcatt gaaaaaccgc aaaacggttc ggttatggtg agggatatcc gatcccagta
    21661 cgtgaatcag tacagtcatg cccgcctacg ccagagtaaa aacacgccac aacaaagaga
    21721 aaaatagacg atataagttg ccatatatgc ctgcgccgcg cccagtgcac catgcgcagg
    21781 gattagccag tgggcaaata ccatcaataa agtgaactgg ctgacttccg ccagaatata
    21841 aaaccgcagt gacgctttgg cgatcaccag ataaccaaag acataagcgc ccacttttaa
    21901 cacatcaccc actaactgcc aggcaaagag atcgcgcata gcggtaaatt tattcgacaa
    21961 cagcagccag atagcaaaat cacgcagcaa ccagacggta aaactcgccg ccgccactgc
    22021 cggtaagacg aatttcagcg atttaaccac ttcccgggtg atatcgcgct tttccgttag
    22081 ccgcgacaac gtgggcagca aatatacgct gaacgatgcc gtaataaatt gcaggtaggc
    22141 atcggaaata ctgctcaccc cttgccagat ccccacctca tcccagctat actgcgctgc
    22201 cagcagtttg cgcatcatga tgtaagcaac aggcaaagtc accgacgtaa tcaacgccat
    22261 gagcgtaaat ttgctcaact gccctgccag accgttatcc cagctgggtt tcagatagct
    22321 taacgggatg acaccacgtt tgatcaacat gatggcggca ggaatgacca ccagcgcggg
    22381 aatcagcgcc agacccagca acgccccttc ataaccgccc aaacggtaac tgacgtagta
    22441 cgcgagaacg ccaatcaagc tgccgacaat cagcgataac gcattacctg cggcatcgcg
    22501 aaagcctttc atcagcgcca gtaacaggtt gccccaggcg atccccattt gcaccagcgc
    22561 cactaaacgc accagcccct gatagtcggt attaccaaac aatccctggc tgattggcgc
    22621 agctgccagc acaaaaacca gcgccatcag cgtagagaag ccaagtacca tcgctgatga
    22681 agtgccgacc acgcggcgca gctgttgggg attatcatgg tactgggcaa cgtatttggt
    22741 tacaccgtta aagatgccag ccccggcaag cacgccgagc acggtaatca actggcggaa
    22801 atttgccgcc agcccaagcc ccgccggacc aaatgacacc gccagcaact taccgaccag
    22861 taacccggca ccaatcttga ccagtgtact ggccgccgtc cacaaggacg cttttgccaa
    22921 cgacatatca ggaaaagtag ttcaacaaag tcgcaattac cgtacgctga ttgacgggcg
    22981 acaggttgta gaacagcggc aggcgcagca ggcgctcgct ctctttggtg gtgtagcgat
    23041 cttcaccgtg gaactcacca aagtgttccc ccgcagggca accgtgcagc ggaatgtaat
    23101 gaaacaccgc catgatttcc gcttctttca gaaagttaat caacgcgctc cggtcatcaa
    23161 tatcccgcag tttaatgtag aacatatgcg cgttctgcac gcagccatcg ggaatcgacg
    23221 gcagctcgat acgcccggct ttcgccagag gcgctaacgc atcgtagtag ttttgccaca
    23281 gcgccagacg ttgctggttg atacgatccg ctgcttccag ttgcgcccac aggtatgcag
    23341 cttgcagatc ggacatcaaa tagctggagc caatatcgcg ccaggtatat ttatcgacct
    23401 gaccacggaa gaactggctg cggttagtgc ccttttcacg gatgatctcg gctcgttcga
    23461 ttaacgcttt atcgttaatc agcgtcgcgc cgccttcacc gcccgccgtg tagtttttgg
    23521 tttcatggaa gctaaagcag ccaatatgac caatggttcc cagtgcacgc cctttgtaag
    23581 tggacatcac gccctgagcg gcatcttcta ccacaaacaa attatgcttt ttcgccaacg
    23641 ccataatggt gtccatttcg caggccacac ccgcgtaatg gaccggcacg ataacgcgcg
    23701 ttttgtcggt gatcgccgct tcaatcagcg tttcgtcgat gttcatggtg tccgggcgaa
    23761 catccacaaa aacgattttt gcgccacgca gcacaaaggc attggcggtg gagacaaagg
    23821 tgtagctcgg catgatcact tcatcgccag gctggatatc gagcagcagc gccgccatct
    23881 ccagcgaagc ggtgcaggac ggcgtcagta acactttggc gctgccaaaa cgttgctcca
    23941 gccactgctg gcagcgacgg gtaaaaccgc catcgccaca cagtttgccg ctacccattg
    24001 ccgactgcat atagtcgagt tcggttccca ccaccggcgg tgcgttaaat ggaatcatgt
    24061 gatcacctgt ataaccagta cgcggtgctt tctacattcg caccactttg tatgtatcgt
    24121 ttaagcgcgg cggtgttgcc catttgggtc gccacccgca aagttgtttt accgcgagca
    24181 tacgcccagt ttagcgccgt ttgcatcagc tcagcacctg caccgcgtcc agccagcagg
    24241 ccaattcgcg catctgtcgc attgagttcc cgtaaagaga catagccgcg aatatcgccg
    24301 gacgccgcac gtaaaatcag acattgatga tcaaaggtgc cgcgcacggc attttcaatc
    24361 cactgtgcat aaaagcgact gctggcgtca ggcgcatacc acggcgcacg aaaacggctt
    24421 tgcgcaaatg cggcgctggc taactgacgt aatgcgggaa tatcggtctc ttgtgccact
    24481 acagcaccgc tatcactggc attgttcacg ggtagcgcca aatcaacttc accttctacc
    24541 agggagaatc ccagctgttg cagggcatcc agttcacccg tatttgatgc cgcaattttg
    24601 gcctgcaccc gtgaccacgg cgctaacgcg tctggcgtca ggagcggtgc ttcagacgta
    24661 atgcgcacga tggcgctgtt aacaccaaag aaggcgtttt cccaggttag tggctcaata
    24721 ctggcgcgga cgggcacgaa gtaactccag cagatattgg ccgtagccag ttttcgctaa
    24781 tgaactggca gcacgcttca caccctcgtc atcgagccag ccgttacgcc aggcgatctc
    24841 ttccaggcag gcaatcttaa agccctggcg tttttctact gtctgcacaa aggtactggc
    24901 ttcaatcagg ctgtcgtgag tgccagtgtc cagccaggca aatccgcgcc cgagcagttc
    24961 aacggtcagg ttgcccgcct cgaggtacat ctggttgatg gaggtaatct ccagttcacc
    25021 acgctccgac ggcttcacct gctttgcgta ctccacgact ttactgtcgt agaaataaag
    25081 cccggtcacc gcccagtttg acttcggctg ttttggcttt tcttccagcg agatagcgcg
    25141 gaaattgtcg tcaaactcca ccacgccaaa gcgttccggg tccatcacct gatagccaaa
    25201 aaccgtcgcc ccttccgtgc gcgccgcaac atgacgcagc ttcggactga agccctgacc
    25261 gaagaaaatg ttatcgccca gcaccagaca agaaggttca ccgttgagga aggtttcacc
    25321 gatgataaag gcctgcgcca gaccgtccgg gctgggctgt tcggcatatt ccagctggat
    25381 accgaactca ctaccatcgc ccagcaggcg ctggaaataa cctttatctt ccggcgtagt
    25441 gatgatgaga atttcgcgga taccggccag catcagcacc gacagcggat agtaaatcat
    25501 tggcttatcg taaatcggca acagttgctt cgatacgccg cgcgtaatcg gatgcaatcg
    25561 ggtgccggaa ccgcccgcca ggataatacc tttcatgttt gcctccgctg aaaattagcc
    25621 tttcagacct aaacgctcgc cctgatagct gccgtcctgc acctgcttcc accagctttc
    25681 attagccaga taccactgca ccgttttacg cattccactt tcaaaggttt cctgtggcag
    25741 ccaaccaagt tcacgggcaa ttttcgaagc atcaatggca taacgcagat catgccccgg
    25801 acggtcagcg acaaaggtga tcaagtcacg atagtgcgcc acgccgtgcg gcttgttcgg
    25861 agccagttct tccagcagct cgcaaatggt ttccacaaca tcgagattct tacgctcgtt
    25921 gtgaccacca atattatagg tttcaccgac tttcccggtg gtcgccacgc aatacagcgc
    25981 gcgggcgtga tcttccacat acagccagtc acggatttgc tgcccgttgc catataccgg
    26041 cagcggttta cccgccagcg cgttgaggat catcagcggg atcagttttt ccggaaagtg
    26101 gtaaggaccg tagttattcg agcagttggt gatcagcgtc ggcagaccgt aggtccgcag
    26161 ccaggcgcgc accagatggt cgctgctggc ttttgacgcg gaataagggc tgctcggcgc
    26221 atacggcgtg gtttcggtga agaaatcatc cgtcgagtgc aggtcaccat atacttcgtc
    26281 ggtggagata tgatgaaaac ggaacgctga ttttttatct tccgtcagcg tattccagta
    26341 agcccgcgcc gcttcaagca atgtataagt cccgacaatg ttggtttcaa taaacgctgc
    26401 cgggccgtca atagaacggt caacatggct ttcggctgcc agatgcatga cacagtctgg
    26461 ctgatgctca gtgaatacgc gtgccagttc tgcccgatcg cagatatcaa ctttctcaaa
    26521 ggcaaagcgc tcgctttgcg cgaccggtgc cagcgacatc aggtttccgg cgtaggtcag
    26581 cttatcgacc actaccaccg cgtcgctcgt ttcgttgatg atataacgca ccagcgccga
    26641 gccaataaac ccggcaccac ctgttatcag aatttttctc atcagcgcca gactcctttg
    26701 gcatcgacga catactgctg atggacattg tcgccattga taactttgaa ctgactatga
    26761 tcgaccagca tcaccagcac gtctgccgtt gccagcgcct cgtcaagctg cgccagagta
    26821 caaagcccgg tcagtttttt cggcaactgg tggatgttag gctcaacaac cagagtttcg
    26881 ccgctatgcc actgggcgat cagttcagcg atttccatcg ccgggctttc gcgcaggtca
    26941 tcaatattcg gtttaaacgc cagaccaaag caggcgattt tcagttcact ggcgcgttta
    27001 tcggtagcag ccaggcaatc agccaccgcc gctttcacct gatcgataac ccagaacggt
    27061 ttgtgatcgt tcacttcgcg cgcggtacgg ataagccgcg cctgctgggg gttctgtgcc
    27121 acgataaacc acggatcaac agcaatgcag tgaccgccca cgccagggcc aggctgaaga
    27181 atattaacgc gagggtgacg attcgccagg cgaatcagtt cccagacgtt aatcccctga
    27241 tcggcacaaa tcagcgacaa ttcattagca aaagcgatat tcacatcgcg gaagctgttt
    27301 tcggtgagct tacacatttc cgccgtccgc gagttagtga cgacacactc accttcgagg
    27361 aaaattttgt acagttcgct ggcgcgggcc gaacaaaccg gcgtcatacc accaatcacg
    27421 cgatcgtttt taatcagctc gaccataacc tggcccggta acacgcgttc cgggcagtaa
    27481 gcaatgttga cgtccgcctg ctcgcccacc tgctgtggga aagtgagatc cggacgcatc
    27541 tctgctaacc attctgccat cttctcggtt gaccccaccg gcgaggtgga ttcaaggatc
    27601 accagcgcgc cttttttcag cactggcgca atggagcgag cagccgattc aacgtaggtc
    27661 atatctggct catgatcgcc tttaaacggc gtaggtacag caatcagcca ggcatccgct
    27721 tcaactggcg tcgtgctcgc tcgtaaaaaa ccgccttcta cggcagtttt tactacgctc
    27781 gccaaatcag gttcgacgat atggatttcg ccacgattga tggtatcaac cgcgtgttgg
    27841 ttgatatcga caccaatcac ctgtttttgc cgtgaggcaa acgcagctgc tgtaggcagc
    27901 ccgatgtaac ccagtccgat aacagaaatg gtcgcaaaac tcatagtgat atccgattat
    27961 tttttaacgc ttccagaatg cgagagcatg cctgaccatc accatacggg ttatgggcgc
    28021 ggctcatagc ttgatattcg ttttcgtctt ttaaaagacg cgtcacttcc tcgacaattc
    28081 gctgcttatc cgtgcctacc agacgcaccg tacccgccgt caccgcttcc ggacgctcag
    28141 tggtatcgcg catcaccagc accggtttcc ccagcgaagg cgcttcttcc tgaatgccgc
    28201 ctgagtcggt caaaatcagc caggcgtggt tcatcagcca gacaaacggt aaatactcct
    28261 ggggatcgat cagaatgaca tttttcacat gccccagaat gcgattgacc ggctctctga
    28321 cgttcgggtt gagatgcacc ggatagacaa tctggatgtc ctggtgcgtg gtggcgatgt
    28381 ctgccagcgc gtggcagatt tcttcaaagc cacgaccgaa actttcacgc ctgtgaccgg
    28441 tcaccagaat catcttttta tcggggtcga taaacgggta atttgccgcc agttctgaac
    28501 gcagcgtgtc gctgctcatc acctgatcac gcacccataa cagtgcatca atgactgtat
    28561 taccagtaat gaagattcgg ctatccgcaa cgttttcacg cagcaagttt tgccgggaag
    28621 tttcggttgg agagaagtga tacatcgcca gatgcccggt caatgtacgg ttagcctctt
    28681 ccggccacgg cgaatagaga tcgcccgtgc gtaaaccagc ctcaacgtga ccaacaggaa
    28741 tacgctgata aaacgccgcc aggctggttg ccagcgtcgt tgttgtgtcg ccgtgaacca
    28801 gcacgacgtc tggtttgaac tcggcaagaa taggttttag cccttccaga atccgacagg
    28861 ttatctctgt caggccctgt cctggctgca ttatgttgag atcgtagtca ggtacaatgg
    28921 aaaagagttt cagcacctga tcgagcatct cccgatgctg cgcagtgacg caaactttag
    28981 cctcaaaaaa aggatctttt gccaacgcat gcaccaacgg agccatcttg atggcttctg
    29041 ggcgcgtacc aaatacagtc agtactttca catcgattct cttcgaataa gcggcgagcg
    29101 cctttgcgct cgcagcagca gtgttgctat ttcgagcaac ggcgggttaa tgcgacacca
    29161 gccccgatca gccccccgac aatgccccac ataatcatca ggaaggcacg acgtgggcta
    29221 tcgcgtttta ccggttcttc cggcgtacgc aaatagcgat aggtctgaaa acgcggatcc
    29281 agggttggac caacattcag ggtgtttaac atggcccgat tctgatcata gtcgagatca
    29341 aaggccggac cgacggcctg taaattttcc agtcgagcct ggagcattgg acgcccaagc
    29401 aggaacattt ctgaatcagg taattcctcg gcaggcacat ctgtcgcact gcgcgaaata
    29461 ttatgctgct cagcaatttt cagcgcctgc tcaatgctgt tcatccggcg gtcgtagatg
    29521 gctttcgcca cctcttcctg acgcttcacc tgagctttca tctggatggt gcgtgccgcc
    29581 catgcgcctt tcagctcatc attcagatgg ctggctgcac gctggctggc aaaagcaaca
    29641 tactgacgta acaggttatt agcgtcaggc gcggtttcgg caataagctt cacgctgtca
    29701 ttgaccgcgc gggtaaagtc tccggggata aactggatgt tgttaatcat ttcatccagc
    29761 aacgccgcat cggctttgct gttgcccacc atccgctgtt tgtaatagtc ggtttgcagc
    29821 cagaactctc tgcgggtatc ccacgaggcc agttgcataa caaactcttt ataggcttcg
    29881 tccatgaccg atggttgatc ggcagaagcc atgtttgaac ggacatccag gttacgcaaa
    29941 aattgctgct gcgagtaata tccccccagc atattcaccg ttggacgatc ggtaatcgcc
    30001 gtcgagctcc actcctgacg agcaaaaaaa gtatacgcca gcgcgattaa cgcaaacgcc
    30061 agccccatgc caataatcca tagcttccca gcccacaagg tacgaaacaa cccacgaata
    30121 tccagttcat tttcagcgtc ttcggccggt ttcccaggca ttggttgtgt catcacatcc
    30181 tcatttattt ggttaaattg gggctgccac cacgatttct acgcagtctg cgttttacgc
    30241 gcttaataaa gcgagcaact ttccaggcac gcttaatgca atatccatag aggaagaatg
    30301 ctagcaaaaa gagcaccagc atgacccact ccgggacaaa atgagaatat tctgccagca
    30361 cgccaatgga agcgagcagt gctgcggcaa gggtaatcag cacaaacgcc tgacgggaag
    30421 taaacccggc acgcatgatc aaatggtgaa tatgctgacg gtcaggagag aatgggctca
    30481 tgcctttacg caggcgacgg tacataatcg ccaccatatc cattagcgga atggcgatta
    30541 tccacaaagc ggtaaccggg ctgatgggat gggttttgcc ctgggtcgtt tcgagcagga
    30601 tccagataac ggtaaaacca atcagcgtac tgcccgcatc acccataaag actttgtagc
    30661 ggcgacccag gataccaagg ttaagcatga tgtatggcag gatggcggcg atcatcgcaa
    30721 agcaccagat tgcgaggctg gtttgcccgt cgaaccacaa aatcataccg attgctgcaa
    30781 acgagacgca ggacaacccg cccagcaagc catcaatgcc atcaaccatg ttgaacgcat
    30841 taatggccgc ccagacggca aatagcgtca ggaagtaacc aaacggtccg agcaccatct
    30901 cccaggagcc aaagatataa cccaggctac tgagataaag tttgccgaac accatcataa
    30961 caatgccaac agcggcctgt atggtggcac ggatttttac gctgatatca aaacggtcat
    31021 ccagcgcgcc aatgaaaaca agcacaccgg cacaagcgag atagagagat gcatgcggaa
    31081 tatagtaatc gacaattccg aacgtgaagc aaatccctgc gtaaaccgaa atccccccaa
    31141 cgagaggtat caatccctgg tgacgtttgc ggaagtttgg tttatccact aaaccgactt
    31201 tttttgccac cttacgggca aaaaacagaa acagtgtcgt gaataaaaaa atactgatga
    31261 gatcagtact cactgtcagt aaattcacaa tgcatgctct cagagaaaat tattagcaga
    31321 agtataacca cgaagacctt tattcagaag ggaaagtccg ttccgaaccc accagtcctg
    31381 tgcaaaaatt aaccatttga tatgcaaatt gcataacatg ccagcaaatt ccaggaattt
    31441 tcctaacatc gccagcgcgg cattaagatt acagatataa aagcaaaacg ccacgtaaac
    31501 acgtggcgtt tttggcataa gacaaattta tgagcgtttc atcatttcga agaaatcgtc
    31561 attggtcttg gtcatggcca gtttattaat gaggaattcc attgcatcga tttcgcccat
    31621 cgggtgaatg attttgcgca ggatccacat tttctgcagt tcttcctgag tcgtgagcag
    31681 ctcttcttta cgggtaccgg aacggttgta gtcgatagcc gggaagacgc gtttttcagc
    31741 gatcttacga gagaggtgca gttccatgtt gcctgtacct ttaaactctt cgtaaataac
    31801 ttcgtccatt ttagaaccgg tatcgataag cgccgtcgcg ataatggtca ggctgccgcc
    31861 ctcttccacg ttacgcgccg caccgaagaa gcgtttcgga cgatgaaggg cattggcgtc
    31921 cacaccaccg gtcaacactt tacctgacgc cggaacaaca gtgttgtaag cgcgcgccag
    31981 acgagtgatg gagtcgagca gaatgataac gtctttcttg tgctcaacca ggcgtttggc
    32041 cttctcgatc accatttccg caacctgaac gtggcgagat gcgggttcgt caaaggtaga
    32101 agcaacaact tcacctttta ccagacgctg catctcggtt acttcttccg gacgttcgtc
    32161 gatcagcaga accatcagca cacaatccgg gtggttgtaa gcaatgctct gagcaatgtt
    32221 ctgcagcagc atagttttac cggctttcgg cggtgccaca atcagaccac gctgaccacg
    32281 accgataggt gatgccagat ccagtacgcg agcggttaaa tcttccgtag aaccgttacc
    32341 acgttccata cgcagacgag agtttgcgtg cagcggggtt aagttctcaa agaggatttt
    32401 gttgcgggcg ttttcaggtt tgtcgaagtt aacttcgtta actttcagca gcgcaaaata
    32461 gcgttcacct tctttcggcg ggcgaatctt accagagatg gtatcaccag tgcggaggtt
    32521 gaaacggcgg atttggctag gggaaacgta gatgtcatca ggaccggcga ggtaggagct
    32581 gtctgcggaa cggaggaaac caaatccatc ctgcaatatc tccagtacgc catcaccaaa
    32641 gatatcttcg ccactctttg cgtgctgctt caggatggca aaaataatgt cctgcttacg
    32701 catacgagcc aggttttcca gccccatatt ttcgccgaga gtgatcagct cagaaaccgg
    32761 cgtattcttt aattcggtaa gattcatagt ggtgtgagtt cttaaacttg gggtaaatct
    32821 cgaacttaat gttgtgaatg gtatggcagg atcatccatg cctgtttacg gtcatcgact
    32881 catgtctttt cgctgcctgg tcacaggaga gtacgcagaa ctgaaacgac aagacggatt
    32941 gagtgacgag ccagaaatct gttcacttcg catttaagat aaaacgggaa gcgttggaaa
    33001 ttacaagatt caaacttaat aaggtatgtt taatacgaag tcaacactaa gttagcatga
    33061 ctcacgccgg gcgtccagtt tttagcgacg gggcacccga acatgaaatt cccttacgcc
    33121 aggttagcgt cgaggaactc tttcaactga cctttagaca gtgcacccac tttggttgcc
    33181 gccacttcac cgtttttgaa cagcagcaga gtcgggatac cacggatgcc atatttcggc
    33241 gcagtgccag ggttttgatc gatgttcagt tttgcaacgg tcagtttgcc ctgatattcg
    33301 tcagcgattt catccagaat cggggcgatc attttgcacg gaccgcacca ctctgcccag
    33361 aaatcgacga ggatcgcccc gtccgctttg agtacatccg tgtcaaaact gtcgtcagtc
    33421 aggtgaataa ttttatcgct catatataac tccacaggaa taagcctggc gtgttggttt
    33481 cgttgttggt gtaacattaa ccaactaaag gttgacttta tttcaccgga tacgctttcg
    33541 taaagcaata gtaagctgat attctaccac actatgagca aaacacattt aacagaacag
    33601 aagttttccg acttcgccct gcatccgaag gttgtagaag cccttgaaaa aaaagggttt
    33661 cataactgta cgcccattca ggcactggcc cttccgctga cgctggcggg tcgtgacgta
    33721 gccgggcagg cgcaaaccgg taccgggaaa acgatggcgt ttcttacgtc aacgtttcat
    33781 tatcttctct ctcatcctgc gattgccgat cgcaaggtga atcaaccacg tgccttaatt
    33841 atggcaccga cgcgtgaact tgccgtgcag atccatgccg acgcagaacc gctggcggaa
    33901 gctaccggcc tgaagctggg tctggcttac ggtggtgatg gctacgacaa acagctgaaa
    33961 gtgctggaaa gcggcgttga cattctgatt ggcactacgg ggcgtttaat tgactacgcc
    34021 aagcagaacc acattaacct cggtgccatt caggtggtgg tactggacga agccgatcgc
    34081 atgtacgatc tgggctttat taaagatatc cgctggctgt tccgccgtat gccgcctgca
    34141 aaccagcgcc tcaacatgct gttctccgcc acgctttcgt accgggtacg tgaactggcg
    34201 ttcgagcaga tgaacaatgc cgaatatatt gaagtggaac cggaacagaa aacgggccac
    34261 cgtattaaag aagagctttt ctacccttct aacgaagaaa aaatgcgttt gctgcaaacg
    34321 ctgatcgaag aagagtggcc agacagagcg attattttcg ccaataccaa acaccgttgt
    34381 gaagagatct ggggccacct ggcggcagat ggtcatcgtg tcggtttgtt gacaggcgat
    34441 gtcgcacaga aaaaacgtct gcgtattctt gatgaattta cccgtggcga tctggatatt
    34501 ctggttgcca ccgacgttgc cgcgcgtggt ttgcatattc cggcagtgac gcacgtcttt
    34561 aactacgatt tacccgatga ctgtgaagat tacgttcacc gtattggtcg tacaggtcgc
    34621 gcaggcgcaa gcggtcactc tatcagcctg gcgtgtgaag agtatgcatt gaatttgcct
    34681 gctattgaga cctatattgg tcactcaatt ccggtaagca aatacaatcc ggacgcattg
    34741 atgaccgatc tgccaaaacc gctgcgcctc acgcgcccac gtacaggcaa tggtccgcgt
    34801 cgtactggcg ctccgcgtaa tcgtcgtcgt tcaggttaaa taaaatgtca tactcaaaat
    34861 cattcaaact gtatcaagat ggcaagagaa tgaatcccga tgagcatact gtagtaagtg
    34921 attcaggtga aagaatgcag ccaacacaga gacagattga aggatgaaga gtatgggttc
    34981 cacctcgtcg ctgtatgcag ccattgatct cggttcgaat agttttcata tgctggttgt
    35041 gcgcgaggtg gctggaagca tccagacgct gacgcgaata aaacgcaaag tgcgtctggc
    35101 tgctggcctg aatagcgaaa atgccctgtc taatgaagca atggagcgcg gttggcaatg
    35161 tctgcgcctg tttgctgaac gtctgcaaga tatccctccc tcgcaaattc gcgttgtcgc
    35221 tacggcgaca ttacgtcttg ccgtcaatgc gggtgatttt attgccaaag cgcaggaaat
    35281 cctcggttgt ccggtacagg taatcagcgg tgaagaggaa gcacgtctga tttatcaggg
    35341 cgttgctcac acaactggcg gtgccgatca gcgcctggtg gtggatatag gcggtgccag
    35401 tactgaactg gtaaccggca cgggtgcaca aaccacctcg ttgttcagcc tgtcgatggg
    35461 ctgcgtcacc tggctggaac gctattttgc cgatcgtaat ctggggcagg aaaattttga
    35521 tgctgcagaa aaagcggcac gcgaagtgtt acgtccggtt gccgatgaat tacggtatca
    35581 cggctggaaa gtgtgcgttg gtgcttccgg caccgtgcag gcgttacagg aagtcatgat
    35641 ggcacagggg atggatgaac gcattaccct ggaaaagttg cagcaattga aacagcgagc
    35701 cattcattgc ggtcggctgg aggaactgga gattgacggg ctgacgctgg aacgtgcgtt
    35761 agtgttcccg agtggtctgg cgatcctgat cgccattttt accgaactga atattcagtg
    35821 tatgaccctg gcgggcggtg cgctgcgtga aggcctggtc tacggcatgt tgcatctggc
    35881 cgtcgagcag gatattcgca gccgtacgct gcgtaatatt cagcgccgct ttatgatcga
    35941 tattgatcag gcgcagcgcg tagccaaagt tgcagctaac ttcttcgatc aggtggaaaa
    36001 cgaatggtat cttgaagcaa taagccgcga tttgctcatc agcgcttgtc agcttcatga
    36061 aatcggcctg agcgttgact tcaaacaagc gccgcaacac gctgcttatc tggtgcgtaa
    36121 cctggatctt cccggtttta cccctgcaca gaaaaaactg ctggcgacgc tactgctcaa
    36181 ccagactaac ccggtcgatc tctcatcgct gcatcagcaa aatgccgtac caccgcgcgt
    36241 cgcagaacaa ctctgccgtt tactccgcct ggccatcatt tttgccagcc gtcgtcgtga
    36301 cgatttagtg ccagagatga cattacaggc taaccatgaa ctgttgacct tgacgcttcc
    36361 gcaaggttgg ctaacccaac atccgctggg taaagagatt attgctcagg aaagccagtg
    36421 gcagagctat gtccactggc cgctggaagt gcattaatga gtaagtgccg gatgcgatgc
    36481 tgacgcatct tttccggcct tgattatttc cctcgttttg ccgccatcat cgctttcaga
    36541 ttcgccagat ggctttgccc tttctgcatc cgttcttcgg cgctgaccac tttgcgctcc
    36601 tgttcccaaa tcagatcatc ctgcggcagc tccagcaaaa agcggctcgg ctccgggcgc
    36661 accagctcgc cgtactgacg gcgttcttta cacagcgtaa aggtcaattc cttctgggcg
    36721 cgggtaatgc cgacataggc cagccgccgc tcctcatcga tattatcttc atcgatgctg
    36781 ctctggtgcg gcaaaaaccc ttcttccata ccgaccatgt agacataagg aaactccagc
    36841 ccttttgacg cgtgcagagt catcagttgc acctgatcca gctcttcttc actctcacca
    36901 cgctccatca tgtcgcgcaa agtaaagcgc gtcaccacct gggtgagcgt catcggctca
    36961 tccagttcac tgccttccag catctccgtc atccagctaa acagttggtt gacgttcttc
    37021 atgcgcattt cggcggcttt cgggctgggc gatgtttcgt acagccagga ttcataatcc
    37081 atgccatgga tcagatcacg caccgcagca atcggctccc gctccgccag gcgctggatt
    37141 tctgccaacc agtgagtgaa gcgggtcaat gcttcataac cacgtccgct aagcgtctga
    37201 ctcaggccca tatcaaagct ggcggtaaac atgcttttat tgcgcgtcat cgcccactca
    37261 cccagctttt tcagcgtcgc cgggccaatc tctcgcttcg gcgtgttaac gatacgcaga
    37321 aatgcgctgt catcgtccgg gttagtcagc acgcgcagat aagccagcaa gtccttgatt
    37381 tcaggacgag agaaaaacga cgtaccacca gaaattttgt acgggatgcg gttttgcatc
    37441 aggaattttt caaacacccg cgactgatgg ttaccgcgat aaagaatggc gtaatctttg
    37501 tactgcgttt tattgacgaa gtgatgggcg atcagctcgc cagtaacgcg ctcggcttca
    37561 tgttcctcgt tattcgcgct taaaactttt agctctgtgc cgtaacccag ttcggagaac
    37621 agacgctttt caaagacgtg cggattattg gcgatcagga tgttcgccgc tttcaaaata
    37681 cgtccggaag agcggtagtt ctgctcaagc ttaatcacct tcagcgccgg aaaatcctga
    37741 ctcagcagca ccaggttttg tggacgtgcg ccgcgccagg agtagatcga ctggtcgtca
    37801 tcgcccacca cggtaaagcg cgcgcggctg cccaccagca gtttcaccag ctcatactgg
    37861 ctggtgttgg tgtcctgata ctcatccacc agcagatagc gaattttgtt ctgccagcgc
    37921 tcgcggactt cttcattgcg ttgcagcagc aacgtcggca ataaaatcag atcatcgaag
    37981 tcgagaacgt tacaggcttt caggtgtgca tcatacagcc cataacaatg ggcaaaaata
    38041 cggtcccgct cgccaatcgc actggctgcc gcctgggacg gtgttttgag atcgttcttc
    38101 cagttagaga tggtcgaaat caactgttgc aggagaactt tgtcatcttc aatcagccct
    38161 tcggtcaact ctttgagcaa cgccagctga tcggtatcgt caaacagcga aaagttcgcc
    38221 ttcatcccca gcgccgcata ctcacgcttg atgatatcca gccccagcgt gtggaaggtg
    38281 gagatcatca gcccacgctc ctctttgcga cccagcgtct gcccgacacg ctctttcatc
    38341 tcgcgcgccg ccttattggt aaaggtcacc gccgcgatat gtcgcgcctg ataaccgcaa
    38401 ccgcggatca gatgagcgat tttattggtg ataacacgag ttttaccgga acccgcgccc
    38461 gccagcacca ggcaggggcc ggtaacgaat tcgacagctt gttgttggcc ggggtttaga
    38521 cgcataggtg tattgctcaa tcttcgaacg gggggaggat tgtagcagaa tcgcatttga
    38581 atgtgaccgc gtgtaagccg ctaaccggtt ggctgcggat gtttaaatca gagagagaag
    38641 accgaaaccg gagttcatcg tgaatccatt agacttagaa aatatcgggt cgtaacgttg
    38701 tgttattact ttttcagagt ttactaagga gaggtgtact cgtggcgatt aatttcagtc
    38761 ctaaggttgg tgaaatactg gaatgcaact ttggtaacta tcctgttagc cagaatggac
    38821 agttcagcac cacgtattac gatggccgca ttcctcctga aatgataaaa aacaggttag
    38881 ttgtcgtgct caacggaaaa attaatggca acgcatgtat cgtcattcca ttatcaacca
    38941 ctcgcgatca tgacaagtta aatcgtggaa tgcatgttga aatagccagt aatgttatca
    39001 atggcctgca attctttgat cagcagattc gttgggccaa agccgacctg gtacaacaag
    39061 tcagtcgaaa ccgattaaat agagcaagga cttacagagg gtatcttaat caatgtttgc
    39121 cacatgagtt ggttgcagac atacagaggg cggtcatcaa gtcaatcaat gccatatcgc
    39181 tcataaattg acaataaaat caccatcccc gataatgact ttgtagccca tagaggccct
    39241 aagttttctc cccttgaggg acctgcgaag cccggatttt ccgggctttt gccatatatg
    39301 taccagcccc tccctgactg acaattacgt tagcatctgc ggatcatgct aaaatcgccg
    39361 cctgacaatt attcactcaa ggcacgatca tggcaaaaac agcagcagca ctgcatatcc
    39421 ttgtaaaaga agagaaactg gctctggatc ttctcgagca gattaagaac ggtgccgatt
    39481 tcggcaagct ggcgaagaaa cactccattt gcccatcagg caaacgcggc ggtgatttag
    39541 gtgaattccg ccagggtcag atggttccgg cgttcgataa agtggttttc tcttgtccgg
    39601 tactggagcc gaccggcccg ctgcacaccc agttcggata tcacatcatt aaggtgctgt
    39661 accgcaacta atagcaaggc cttctccagg agaaggcctt gagtgttttc tccctctccc
    39721 tgtgggagag ggtcggggtg agggcatcag cgcgcactta acccgcaaca gcaatacgtt
    39781 tcatatctgt catatagccg cgcagtttct tacctacctg ctcaatcgca tggctgcgaa
    39841 tcgcttcgtt cacatcacgc agttgcgcgt tatctaccgc gccttccgga atagctttac
    39901 ccaagtcgcc cggttgcagc tctgccataa acggtttcag caacggcaca caagcgtaag
    39961 agaacagata gttaccgtac tcagcggtat cagagataac cacgttcatt tcgtacagac
    40021 gcttacgggc gatggtgttg gcaatcagcg gcagctcgtg cagtgattca taatatgcag
    40081 actcttcaat gatgccggaa tcgaccatgg tttcgaacgc cagttcaacg cccgctttca
    40141 ccatcgcgat catcagtacg cctttatcga agtactcctg ctcgccgatt ttgccttcat
    40201 actgcggcgc ggtttcgaag gcggttttgc cggtctcttc acgccaggtc agcagtttct
    40261 tatcatcgtt ggcccagtcc gccatcatac cggaggagaa ttcgccggag atgatgtcgt
    40321 ccatatgttt ctggaacagg ggtgccatga tctctttcag ctgttcagac agcgcgtaag
    40381 cacgcagttt cgccgggtta gagagacggt ccatcatcag ggtgatgccg ccctgcttca
    40441 gcgcttcggt gatggtttcc caaccgaact gaattagttt ttctgcgtat gccggatcgg
    40501 tgccctcttc caccagcttg tcgaagcaca gcagcgagcc agcctgcaac ataccgcaca
    40561 ggatggtttg ctcacccatc aggtcagatt tcacttccgc aacgaaggac gattccagca
    40621 cgcccgcacg gtgaccaccg gttgctgccg cccatgcttt agcgatcgcc ataccttcgc
    40681 ctttcggatc gttttccggg tgaacggcaa tcagcgtcgg tacgccgaat ccacgtttgt
    40741 actcttcacg cacttcggtg ccagggcatt tcggcgcaac catgactacg gtgatatctt
    40801 tacggatctg ctcgcccact tcgacgatgt tgaaaccgtg cgagtaaccc agcgccgcgc
    40861 cgtctttcat cagtggctgt acggtgcgca ctacatcaga gtgctgcttg tccggcgtca
    40921 ggttaaccac cagatccgcc tgcgggatca gttcttcgta agtaccaact ttaaaaccgt
    40981 tttcggtcgc tttacgccag gacgcgcgct tctcggcaat cgcttcttta cgcagagcgt
    41041 aggagatatc gagaccagaa tcacgcatgt tcaggccctg gttcagcccc tgtgcgccac
    41101 agccgacgat aaccactttt ttaccctgaa ggtagctcgc gccgtcggcg aattcgtcgc
    41161 gccccataaa gcgacatttg cccagctgtg ccagctgctg gcgcaggttc agtgtattga
    41221 agtagttagc catggtgatt cctcgtgatg ttgtgcttct tattgttcgg ttcgcgtttg
    41281 cgagattgaa ttcactatat gacaggaaat ttattgcgga aattgatata ttcacaacgt
    41341 cacattgcaa tttctgcaac gtcaacattg agggctgcgc cattggattt acgcgatctg
    41401 aaaaccttcc tgcatctggc ggaaagccgc cattttggcc gcagcgcgcg ggcgatgcac
    41461 gttagcccat ccacgctctc acggcagatt cagcgcctgg aagaagatct tggtcagccg
    41521 ctatttgtgc gcgataaccg cacggtaacg ctgaccgaag cgggcgaaga gttacgcgtt
    41581 ttcgcccagc aaacattgtt gcagtatcag caactgcgcc acaccatcga tcagcaaggg
    41641 ccgtcgctct ctggcgagtt acatattttc tgctcggtga ccgccgctta cagccatcta
    41701 ccgccgattc tcgaccgctt ccgcgcggaa cacccgtcgg tggagatcaa actcaccact
    41761 ggtgatgcgg cagatgcaat ggaaaaagtg gtcacgggag aagcggatct ggcgattgcc
    41821 gggaaaccgg agacgctacc cggcgctgtg gcgttttcga tgctggagaa tctggcagta
    41881 gtgctgattg cccccgcgct gccctgcccg gtgcgtaatc aggtatctgt cgaaaagccc
    41941 gactggtcaa cggtgccgtt tattatggcc gatcaggggc cggtacgccg ccgcattgaa
    42001 ctgtggtttc gacgcaataa aatcagcaac ccgatgattt acgccacggt tggcgggcat
    42061 gaagcgatgg tatcgatggt ggcgctcggc tgtggtgtgg cgctgttgcc ggaagtggtg
    42121 ctggaaaaca gcccggagcc tgtacgcaac cgggtaatga ttttagatcg cagcgatgag
    42181 aaaacgccgt ttgaactggg cgtgtgtgca caaaaaaagc ggctgcatga gccgctgatt
    42241 gaggcgttct ggaggatttt gccgaaccac aaatgacgtt gtcgcgcggg taggcctgat
    42301 aagcgaagcg ctatcaggca tttttcccta acccgccaaa aagaacctga acgccggatt
    42361 attggtttcg tcgtggcaat cgtagcccag ctcatttagc cgggtttcga aatccggttc
    42421 atggtcgccg agttcgaacg ccgccagtac gcgcccgtag tcggtgccat ggctgcgata
    42481 gtggaacaaa gaaatgttcc agtgcgtacc cagcgtgttg aggaagcgca gcagcgcgcc
    42541 cggtgattcc gggaattcga agctgtagag gcgttcctgc aacggatgtg acggacgccc
    42601 gccgaccata tagcgcacat gcaacttcgc catttcgtcg tcggagagat ccaccacgct
    42661 gtagccgccg tcgttgagca tctgcaaaat ttctttgcgc tcttcgaggc cgcggctcag
    42721 gcgcacgccg acaaagatgc aggcgttttt ggcatcggca aaacggtagt tgaactcggt
    42781 gaccgatcgc ccgccaagca gttggcagaa tttgaggaag ctgccttttt cttccggaat
    42841 ggtcaccgcc aacaacgctt cacgctgttc gcccagttcg cagcgttccg agacgtagcg
    42901 caggccgtgg aagttcacgt tggcaccgga aagaatatgc gccagccgtt cgccgcgaat
    42961 gttgtgctgg gcgatatatt ttttcattcc cgccagcgcc agcgcgccag agggttccgc
    43021 caccgcgcgc acatcttcga ataaatcctt catcgccgca cagatcgcat cactatcgac
    43081 ggtgatgatg tcgtcgagat actcctggca taaacggaag gtttcgtcac cgatgcgttt
    43141 taccgctacg ccttcagcaa atagccctac gcgcggcaga tcaaccggat gacccgcatc
    43201 cagcgctgct ttcaggcagg cggagtcttc cgcttctacg gcgatcactt tgatttgtgg
    43261 catcagttgt ttgatcagca ccgcaacgcc cgccgccaga ccgccgccgc cgactggcac
    43321 aaatacgcgg tcgagatggg cgtcctgctg gagcagttcc agcgccagcg tgccttgccc
    43381 ggcaatcacc atcggatggt cgaacggcgg cacccaggtg aacccctgct gctgtgacag
    43441 ttcgatcgct ttagctttcg cttcgtcaaa gttcgcgcca tgaagcagca cttcgccgcc
    43501 gaatccacgt accgcatcga ctttgatgtc ggcggtggcg gttggcataa cgatcagggc
    43561 cttcacgcct aaccgcgcag aagaaaacgc gacgccctgc gcgtggttac ccgcagaagc
    43621 agtgatcacg ccgtgcgctt tttgttcttc cgtcaggccc gccatcatgg cgtatgcgcc
    43681 gcgcagctta aagctgtgca ctggctggcg atcttcgcgc ttcaccagaa tgacgttatc
    43741 aagacgcgac gacagttttt ccattttttg tagcggcgta acctgcgccg cctcgtaaac
    43801 cggcgcgcgc agcactgctc ttaaatattc ggcaccttcc ggagcaccgg acaggggttg
    43861 cgagtcagcc attattaacc ccccagtttc gatttatcgc gcaccgcacc tttgtcggcg
    43921 ctggttgcca ggctggcgta ggcgcgcagg gcaaaggaaa cctgacgttc acggtttttc
    43981 ggcgtccagg ctttgtcacc ccgagcttcc tgcgcttcac gacgcgccgc cagttcggca
    44041 tcgcttacct gtaactgaat gccacggttc gggatgtcga tagcgatcag gtcaccatct
    44101 tcaatcagac caatgctgcc gccgcttgcc gcttccggtg agacgtggcc gatggaaaga
    44161 ccagaggtgc cgccagagaa acgaccgtcg gtgatcagcg cacaggcttt accgagaccc
    44221 attgatttca ggaagctggt tgggtagagc atttcctgca tgccagggcc gcctttcggg
    44281 ccttcatagc ggatcaccac cacatcgccc gcgacaactt tgccaccgag gatcgcttct
    44341 accgcgtcgt cctggctttc gtacactttc gccgggccgg tgaatttgag gatgctgtca
    44401 tcgacgcccg cggttttaac gatgcagccg ttttctgcga aattaccgta gagcaccgcc
    44461 aggccgccgt ctttgctgta ggcgtgttcc agcgagcgga tacagccatt ggcgcgatcg
    44521 tcatcgagag aatcccaacg gcaatcctgc gagaatgcct gtgtggtacg aatgcctgca
    44581 ggacctgcgc ggaacatatt ttttaccgcg tcatcctggg tcagcataac gtcgtattgt
    44641 tccagcgttt gcggcaacgt caggccaagt acgtttttca catcacggtt cagtaacccc
    44701 gcgcgatcca gttcgccgag aataccgata acaccaccag cacggtgaac atcttccata
    44761 tggtatttct gggtgctcgg cgcaacttta cacagctgtg gaaccttgcg ggaaagctta
    44821 tcgatatcac tcatggtgaa gtcgatttcc gcttcctgcg ccgccgccag caggtgaagt
    44881 acggtgttag tcgatccacc catcgcgata tccagcgtca tggcgttttc aaacgccgcc
    44941 ttactggcga tattacgcgg cagtgcactt tcgtcgtttt gctcgtaata acgtttggtc
    45001 aattcaacaa tgcgtttacc agcattaagg aacagctgct tacggtcggc gtgggttgcc
    45061 agcagcgagc cgttgcccgg ctgcgacagg cccagcgctt cggtcaggca gttcattgag
    45121 ttagcggtaa acatcccgga gcaggaaccg caggttgggc aggcggaacg ttcaacctga
    45181 tcgctctggg agtcagaaac tttcgggtct gcgccctgga tcatcgcatc aaccagatcg
    45241 agtttgatta tctgatcgga cagtttggtt ttcccggcct ccatcgggcc gccggaaaca
    45301 aagatcaccg gaatattcag gcgcagggaa gccatcagca tccccggggt gattttgtcg
    45361 cagttagaga tgcagaccat ggcgtcggcg cagtgggcgt tgaccatata ctcaacggaa
    45421 tcagcgatca gttcgcgaga tggcagtgaa taaagcatcc ccccgtggcc catggcaatc
    45481 ccatcatcca ccgcaatggt gttgaactct ttggcaacgc cgccagccgc ttcaatttgt
    45541 tcggcgacca gtttaccgag atcgcgcaga tggacgtgac ccggtacaaa ttgggtgaac
    45601 gagttcacaa ccgcgataat cggcttaccg aaatcggcgt cggtcattcc ggtggcgcgc
    45661 cacagcgcac gagcacccgc catattacga ccatgagtgg tggtggcgga acggtactta
    45721 ggcatacttt atttactccc agtgtctgtc tcgtaaatgg gacggtgcgt gccgtcccat
    45781 tttttgtatt tattgattaa cttgatctaa ccagccccat ttatcttcgg tttcgccagt
    45841 gaagaggccg aagaaggctt gctgaatgcg tttggtaacc gggccacaac ggccttcgcc
    45901 aacctgaata ccgtctacgc tgcgcactgg cgtgatttct gccgccgtac cggacataaa
    45961 cacttcatcc gccaggtaca gggattcgcg cgacagcacc tgctcacgta cttcaattcc
    46021 cagctctttc gccagtttga tgatggcatc acgggtaata cccggcagcg cggaggaggt
    46081 gaacggtggg gtgaacagca caccatcttt cacttcaaac aggttttcgc ctgcgccttc
    46141 agagatataa ccgttcacat ccagcgcgat accttcctga taaccgtggc ggcgcgcttc
    46201 gctacccacc agcagggaag agaggtagtt accaccggct tttgccgccg tcgggatggt
    46261 gtttggtgct gcgcggttcc aggaggaaac catcgcatcg atcccctgct ccagcgcttc
    46321 tgcgcccaga tacgctcccc acgggaaagc agcgataatc acgtcggttg agtatcccgc
    46381 tggcgggttt actcccatgc caacatcacc gacgaagatc agcggacgga tataggcgct
    46441 ggtgagatta tttttgcgga tcacgtcacg acaagcttcc atcagctcat caatgctctg
    46501 cgaaaccggg aagcgataga ttttggcgga gtcatgcaga cgctgcatat gctcacgatg
    46561 gcggaataca accggtcctt tgtgcgagtc gtagcaacgg atgccttcaa aaaccgaagt
    46621 gccatagtgc agcgcgtgcg acatcacatg caccttcgcg tcttcccagc gaaccatctc
    46681 cccattgaac caaatgtaat cagctttctt cgtggtcatt tttatattcc ttttgcgctc
    46741 aggcgcggat ttgttgtgat gtggttgtgc tctggcagat ggcaacgtgt gcgacgtcca
    46801 ccagtttatt taactgacta aacagtaagt cgaccgaccg tgggctggca acggtcaatt
    46861 cgatatttat attttgtgca tcgctggcgg cggccatatt cattgagcag acgtggaaac
    46921 cacgatgacg caccacgcgt aaaacacgtt ctaaggtttc cggattgaag cgagccgata
    46981 cattgacctg atgttgcatc atgataattt ctccaacatt tctgaattac tgtcaccagg
    47041 cggcaccagc ggccagacgt tctcaagttc gtcgattgag acatgaagca ggtatggccc
    47101 atcactgttc agcatggtgt cgagtgccgc ttcaacctgg tctttacggg tgatgtgttg
    47161 gccagggatg ccgaaggcgc tggctaacat gagaaaatcg gggttatcgg taagggtggt
    47221 ttcgctatat cgttcctgga aaaacagttg ctgccattgt cgaaccatcc ctaaccgttg
    47281 gttatcgagt aagacgattt tcaacggtaa ctgcttacgt tttacggtgc ccagctcttg
    47341 tacattcatc atgaaagagc cgtcaccgga gatacagacg acggtatcgt tcggtcgcgc
    47401 gacttgcgcg ccaaccgccg ccggtaaacc gaaacccatg gtgcctaagc cgctggaggt
    47461 gatgaaattt tccgggcgag tatgggcgat gtgctgcgca gcccacatct ggtgctgccc
    47521 cacatctgtg gtcacgacgc aatccgcagg tttacgatcc gacagttgtt ttaacaacaa
    47581 tggcgcgtag atagcgtcac cgggatggtc gtaacgccag gcatgttcat cacgcagctg
    47641 cgcgcagtgt agctgccagt cattgatatt taacggctgc tgtaatgctg gtaacagagc
    47701 atttaaatca ccttgtaatg ccacatgtgc ctgacgcagc ttgttcattt ctgccgggtc
    47761 gatatccata tggataacac tggcgtgtgg tgcgaaggtg ttcagtttgc cggtcacccg
    47821 gtcatcaaaa cgtgcaccca cggcgatcag caagtcgcac tcctgcaccg cgaagttcgc
    47881 cgctttggtg ccatgcattc ccagcatgcc cagatagtac ggataatctg cttcaactgc
    47941 gcccagccct ttcagcgtgc aggtggcagg catttttgtg gtagcgagaa attctcgtaa
    48001 agcaggaact gcctgcgcca tacccacgcc accaccaacg tacagcatcg gtttttgcgc
    48061 ttttgccagc atctggcgcg cttgctcgac ttcggcatgt gggaaagtca cttcgttttc
    48121 aacggtggtg aaccacggtt ccaggtcacc gctggctagc tggatatctt ttgggatatc
    48181 gaccagaacc ggaccaggac gacctgagct ggcaacgtcg aatgcttcag ccatgatgcg
    48241 cggcaattct tccagcgact gcaccagaaa gctgtgcttg gtacaggcta acgacaatcc
    48301 caggacatcc acttcctgaa atgcgtcagt gccgataaac ggtgcggaca cttgaccggt
    48361 gatggcaaca acagggatag aatctaacag tgcgtccgca agcccggtta tcaggttggt
    48421 tgcgcccgga ccagacgtgg cgatacatac gccagttttg ccggtagcac gggcataacc
    48481 gatagccgcc attgccgcac cctgctcatg tcggcacagc aagtgctcca cgccgccgtc
    48541 atacaatgca tcgtaaaccg gcataattgc gccacccgga taaccgaaaa cggtgttcac
    48601 accctgtgcc cgcaacgcat gtaccaccca ctgtgcgcca ttcatagtta gttccccgtc
    48661 ctgaatcttg agaaacagaa ttttgtgctg ttattcattg tctgctcctc ggttatgttt
    48721 ttaaggtcaa aaaaaacccc cggacctttc ggtgcggggg tcttagttcg ttaaggcttg
    48781 atctctaagc ctttcctcgt ccaagtgcag ccccgcacgg tgggataata atcaccacca
    48841 cgctaatcac gaccaggcta atcactcgta gaagggctgt cattttgtct tttcttgcat
    48901 cttgttcgaa ggaatgccta aagagttacc ataggttttg taaatagtac aagatatttt
    48961 ttggccgatt tttatgatgc gtttaataat ttcaatataa attgttgttt tttaaaaata
    49021 aaatagtgag tgaaaaaaat tcaatttcag tatgaatgtt gttcccttcc tcgtagttca
    49081 tcctttgtcc gctgcatttt tgcgagcatc attccaccgc cagaacaact caacttcact
    49141 tgcacaaata aacaggaatc agccactaaa attcacgaat tagcgggcta cagcagcgat
    49201 atcttatgca cataatggtg actcaaggag ggcttatgtc actgtcaatt gttcataccc
    49261 gcgcagccct gggagtaaat gcgcccccga tcactgttga ggtacatatc agtaaaggtc
    49321 tacccggctt aacgatggtg ggcttaccag aaacaacggt aaaagaagct cgcgatcgcg
    49381 tgcgcagcgc cattatcaat agcggatatg aatatccggc gaaaaaaatc accatcaacc
    49441 tggctccagc cgatctgcca aaagaagggg gacgatatga tttacctatc gccattgcgt
    49501 tgctggcggc ctcagaacag cttacagcca ataagttaga tgaatatgaa ttagtcggag
    49561 aactggctct tacaggcgct ctgcgtggcg ttcccggtgc aatctccagt gcaactgaag
    49621 ctattaagtc gggcagaaaa attatcgtcg cgaaagataa cgaagatgaa gtggggctaa
    49681 ttaacggtga aggatgcctg atagccgatc atctgcaagc tgtctgtgcg tttctggaag
    49741 gtaagcacgc tctcgaacgc ccgaaaccaa ctgatgcagt atcccgggcg ctacaacatg
    49801 atctcagtga tgttgtcggt caggaacaag gaaagcgagg actggaaatt accgccgctg
    49861 gcgggcacaa ccttttactg attgggccgc cgggaacagg taaaacaatg ctcgccagcc
    49921 gtattaatgg cctgttgcca gatttaagca atgaagaggc actggagagc gctgcgatat
    49981 taagtctggt aaatgctgaa tcagtacaaa aacaatggcg gcagcgcccg ttccgctcac
    50041 ctcatcacag tgcgtcgtta actgcgatgg tgggcggtgg tgcaattcca gggccaggtg
    50101 aaatttcgtt ggcgcataac ggcgtgcttt ttcttgatga gctacctgaa tttgaacggc
    50161 gtacactgga tgccttgcga gagccgattg aatccgggca gatccatctt tcacgcacac
    50221 gagcaaaaat aacctatcca gcccgtttcc agcttgttgc ggcgatgaat cccagcccta
    50281 ccggacatta tcagggaaac cataaccgct gcacgccaga acagacatta cgttatctca
    50341 accggctctc ggggcccttt ctcgaccgct tcgatctctc actggagatc ccattaccac
    50401 cccccggcat tttgagtaaa acggtagtgc cgggagaaag cagcgccacc gttaaacaac
    50461 gcgtaatggc cgccagagag cgccaattta agcggcagaa taaactgaat gcctggctgg
    50521 atagtccgga aatacgccaa ttctgcaaac ttgagagcga agatgcgatg tggttggaag
    50581 gaacactgat ccatctgggg ttatcgattc gtgcctggca gcggttattg aaagttgcac
    50641 gaaccattgc tgatattgat cagtctgaca ttatcacacg tcagcatttg caggaggcag
    50701 ttagctatcg tgcgattgac cgtttgctca tccatctgca aaaactactg acataaaaaa
    50761 agggcatttc gcccttttta ttaatcatca gaatcggtgt agtcttcagc accttcaacc
    50821 tgcggtttac cgccggaaag ggtgtgaaaa cgttttggac gcttaatacg cgtcatatac
    50881 ttggaccaca cgcgttctgc ttctgtcact ggctcacgtt cgccacggca tactgctacg
    50941 aagagtttct cttcctcggt aaccggctcg cgtttgccaa gatccaactc attgaaggca
    51001 taaccatgac gctcaagcag ttgtgcctct ttgatggtga aatcaccatg acgagagaat
    51061 ccacgtggat aatgtttatt gtcgaaatat cgattagtcg tcgtaaagct ttccgccatc
    51121 ctgcacgctc ctaattcttt gaccgagcta gttatggcgc ggagtattag ttacgcttga
    51181 cagagtgtaa aacaaaacat ttaaatcata acgacaaata attttgcgga gagcactgtg
    51241 gatacggaat tgttaaaaac tttcctggaa gttagccgaa cgcgtcactt tggtcgagcg
    51301 gctgaatcgc tctatctgac ccagtcagca gtgagctttc gaatcagaca actggaaaat
    51361 caactgggtg tgaacctttt cacccgccac agaaacaata tccgtttaac cgctgccggt
    51421 gaaaaactac tgccttatgc agaaacgctc atgagcacct ggcaggccgc ccgtaaggag
    51481 gtggcgcata cctcacgaca taacgagttt tctatcggtg ccagcgcctc gttgtgggaa
    51541 tgtatgctta atcagtggct gggacgcttg tatcaaaatc aggatgccca tacaggctta
    51601 cagttcgaag cgcgaattgc ccaacggcag tctctggtaa aacagctgca tgaacgccag
    51661 cttgatcttc ttattaccac tgaagcgccc aaaatggacg aatttagtag tcagttgctg
    51721 ggatatttca ctttagcgct ttataccagt gccccttcaa aactaaaggg agatcttaat
    51781 tatctgcgac ttgagtgggg gccagatttt caacagcatg aggcaggttt gatcggtgct
    51841 gacgaagtgc ccattctgac aaccagttct gctgaactgg cacagcaaca gattgcgatg
    51901 cttaatggtt gcacctggct acccgtcagc tgggcgcgta aaaaaggcgg cctgcatacc
    51961 gttgtcgata gcacaacact ttcacggccg ctttatgcca tatggctgca aaatagcgat
    52021 aaaaatacgt tgattcgcga tcttttgaaa attaacgtgc tggatgaagt gtattaatat
    52081 gaatggctgg caaggatgcc ggtagaagga tttacttcgg agagggttat ttcagataaa
    52141 aaaaatcctt agctttcgct aaggatgatt tctggcaggg gcggagagac tcgaactccc
    52201 aacacccggt tttggagacc ggtgctctac caattgaact acgcccctaa ttagggtggc
    52261 ggaacggacg ggactcgaac ccgcgacccc ctgcgtgaca ggcaggtatt ctaaccgact
    52321 gaactaccgc tccaccgaat tcttttacaa ccaccggttt tatgaccggc ttactgctta
    52381 atttgatgcc tggcagttcc ctactctcgc atggggagac cccacactac catcggcgct
    52441 acggcgtttc acttctgagt tcggcatggg gtcaggtggg accaccgcgc taaggccgcc
    52501 aggcaaattc tgttttatca gaccgcttct gcgttctgat ttaatctgta tcaggctgaa
    52561 aatcttctct catccgc
//
LOCUS       DAJOWB010000019        49396 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJOWB010000019 DAJOWB010000000
VERSION     DAJOWB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.019"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(26..925)
                     /gene="pgrR"
                     /locus_tag="OHO85_001277"
     CDS             complement(26..925)
                     /gene="pgrR"
                     /locus_tag="OHO85_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCQ2670680.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            1063..1983
                     /locus_tag="OHO85_001278"
     CDS             1063..1983
                     /locus_tag="OHO85_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCQ2670681.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDSRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            2054..2191
                     /locus_tag="OHO85_001279"
                     /pseudo
     CDS             2054..2191
                     /locus_tag="OHO85_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
     gene            2319..3047
                     /gene="mpaA"
                     /locus_tag="OHO85_001280"
     CDS             2319..3047
                     /gene="mpaA"
                     /locus_tag="OHO85_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCQ2670682.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCANPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(3022..3987)
                     /gene="ycjG"
                     /locus_tag="OHO85_001281"
     CDS             complement(3022..3987)
                     /gene="ycjG"
                     /locus_tag="OHO85_001281"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCQ2670683.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADIDGPTWLAVDVEPALQFTTGELHL"
     gene            4106..4612
                     /gene="tpx"
                     /locus_tag="OHO85_001282"
     CDS             4106..4612
                     /gene="tpx"
                     /locus_tag="OHO85_001282"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCQ2670684.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(4656..6197)
                     /gene="tyrR"
                     /locus_tag="OHO85_001283"
     CDS             complement(4656..6197)
                     /gene="tyrR"
                     /locus_tag="OHO85_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCQ2670685.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(6345..7406)
                     /locus_tag="OHO85_001284"
     CDS             complement(6345..7406)
                     /locus_tag="OHO85_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCQ2670686.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(7403..8800)
                     /locus_tag="OHO85_001285"
     CDS             complement(7403..8800)
                     /locus_tag="OHO85_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCQ2670687.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8955..9953
                     /locus_tag="OHO85_001286"
     CDS             8955..9953
                     /locus_tag="OHO85_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCQ2670688.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(10064..10969)
                     /gene="ompG"
                     /locus_tag="OHO85_001287"
     CDS             complement(10064..10969)
                     /gene="ompG"
                     /locus_tag="OHO85_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HCQ2670689.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHFAGVGVNYSF"
     gene            complement(11014..12096)
                     /locus_tag="OHO85_001288"
     CDS             complement(11014..12096)
                     /locus_tag="OHO85_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2670690.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIALR"
     gene            complement(12110..12769)
                     /gene="pgmB"
                     /locus_tag="OHO85_001289"
     CDS             complement(12110..12769)
                     /gene="pgmB"
                     /locus_tag="OHO85_001289"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCQ2670691.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISLDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQN
                     V"
     gene            complement(12766..15033)
                     /gene="ycjT"
                     /locus_tag="OHO85_001290"
     CDS             complement(12766..15033)
                     /gene="ycjT"
                     /locus_tag="OHO85_001290"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCQ2670692.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(15030..16085)
                     /locus_tag="OHO85_001291"
     CDS             complement(15030..16085)
                     /locus_tag="OHO85_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCQ2670693.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLRDQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(16095..16883)
                     /locus_tag="OHO85_001292"
     CDS             complement(16095..16883)
                     /locus_tag="OHO85_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCQ2670694.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(16901..17953)
                     /locus_tag="OHO85_001293"
     CDS             complement(16901..17953)
                     /locus_tag="OHO85_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCQ2670695.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17984..18826)
                     /locus_tag="OHO85_001294"
     CDS             complement(17984..18826)
                     /locus_tag="OHO85_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCQ2670696.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(18813..19694)
                     /locus_tag="OHO85_001295"
     CDS             complement(18813..19694)
                     /locus_tag="OHO85_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCQ2670697.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(19715..21007)
                     /locus_tag="OHO85_001296"
     CDS             complement(19715..21007)
                     /locus_tag="OHO85_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2670698.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELVEQH"
     gene            complement(21021..22700)
                     /gene="ycjM"
                     /locus_tag="OHO85_001297"
     CDS             complement(21021..22700)
                     /gene="ycjM"
                     /locus_tag="OHO85_001297"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCQ2670699.1"
                     /translation="MKQKITDHLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVYLLPFYPWSSDDGFSVID
                     YQQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(22913..23227)
                     /gene="pspE"
                     /locus_tag="OHO85_001298"
     CDS             complement(22913..23227)
                     /gene="pspE"
                     /locus_tag="OHO85_001298"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCQ2670700.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(23302..23523)
                     /gene="pspD"
                     /locus_tag="OHO85_001299"
     CDS             complement(23302..23523)
                     /gene="pspD"
                     /locus_tag="OHO85_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCQ2670701.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(23532..23891)
                     /gene="pspC"
                     /locus_tag="OHO85_001300"
     CDS             complement(23532..23891)
                     /gene="pspC"
                     /locus_tag="OHO85_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCQ2670702.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGVANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(23891..24115)
                     /gene="pspB"
                     /locus_tag="OHO85_001301"
     CDS             complement(23891..24115)
                     /gene="pspB"
                     /locus_tag="OHO85_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCQ2670703.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(24169..24837)
                     /gene="pspA"
                     /locus_tag="OHO85_001302"
     CDS             complement(24169..24837)
                     /gene="pspA"
                     /locus_tag="OHO85_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCQ2670704.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKDREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            25004..25981
                     /gene="pspF"
                     /locus_tag="OHO85_001303"
     CDS             25004..25981
                     /gene="pspF"
                     /locus_tag="OHO85_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCQ2670705.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(26112..27377)
                     /gene="puuE"
                     /locus_tag="OHO85_001304"
     CDS             complement(26112..27377)
                     /gene="puuE"
                     /locus_tag="OHO85_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCQ2670706.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLTNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALRD"
     gene            complement(27415..28695)
                     /gene="puuB"
                     /locus_tag="OHO85_001305"
     CDS             complement(27415..28695)
                     /gene="puuB"
                     /locus_tag="OHO85_001305"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCQ2670707.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTATGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKMLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(28697..30184)
                     /gene="puuC"
                     /locus_tag="OHO85_001306"
     CDS             complement(28697..30184)
                     /gene="puuC"
                     /locus_tag="OHO85_001306"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCQ2670708.1"
                     /translation="MNFHHLAYWQDKALSLAIETRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(30334..30891)
                     /gene="puuR"
                     /locus_tag="OHO85_001307"
     CDS             complement(30334..30891)
                     /gene="puuR"
                     /locus_tag="OHO85_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCQ2670709.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(30918..31682)
                     /gene="puuD"
                     /locus_tag="OHO85_001308"
     CDS             complement(30918..31682)
                     /gene="puuD"
                     /locus_tag="OHO85_001308"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCQ2670710.1"
                     /translation="MENIMNNPVVGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            31893..33311
                     /gene="puuA"
                     /locus_tag="OHO85_001309"
     CDS             31893..33311
                     /gene="puuA"
                     /locus_tag="OHO85_001309"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCQ2670711.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            33460..33624
                     /locus_tag="OHO85_001310"
     CDS             33460..33624
                     /locus_tag="OHO85_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670712.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            33614..34999
                     /gene="puuP"
                     /locus_tag="OHO85_001311"
                     /pseudo
     CDS             33614..34999
                     /gene="puuP"
                     /locus_tag="OHO85_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
     gene            35133..35378
                     /locus_tag="OHO85_001312"
     CDS             35133..35378
                     /locus_tag="OHO85_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCQ2670713.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            35691..37334
                     /gene="sapA"
                     /locus_tag="OHO85_001313"
     CDS             35691..37334
                     /gene="sapA"
                     /locus_tag="OHO85_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCQ2670714.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYDPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            37331..38296
                     /gene="sapB"
                     /locus_tag="OHO85_001314"
     CDS             37331..38296
                     /gene="sapB"
                     /locus_tag="OHO85_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCQ2670715.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            38283..39173
                     /gene="sapC"
                     /locus_tag="OHO85_001315"
     CDS             38283..39173
                     /gene="sapC"
                     /locus_tag="OHO85_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCQ2670716.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            39173..40165
                     /gene="sapD"
                     /locus_tag="OHO85_001316"
     CDS             39173..40165
                     /gene="sapD"
                     /locus_tag="OHO85_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCQ2670717.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            40167..40973
                     /gene="sapF"
                     /locus_tag="OHO85_001317"
     CDS             40167..40973
                     /gene="sapF"
                     /locus_tag="OHO85_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCQ2670718.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            41041..41394
                     /locus_tag="OHO85_001318"
     CDS             41041..41394
                     /locus_tag="OHO85_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCQ2670719.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            41762..42550
                     /gene="fabI"
                     /locus_tag="OHO85_001319"
     CDS             41762..42550
                     /gene="fabI"
                     /locus_tag="OHO85_001319"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCQ2670720.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            42694..43821
                     /locus_tag="OHO85_001320"
     CDS             42694..43821
                     /locus_tag="OHO85_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCQ2670721.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            43889..45823
                     /gene="rnb"
                     /locus_tag="OHO85_001321"
     CDS             43889..45823
                     /gene="rnb"
                     /locus_tag="OHO85_001321"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCQ2670722.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(45901..46005)
                     /locus_tag="OHO85_001322"
     CDS             complement(45901..46005)
                     /locus_tag="OHO85_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670723.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            46059..48044
                     /gene="pdeR"
                     /locus_tag="OHO85_001323"
     CDS             46059..48044
                     /gene="pdeR"
                     /locus_tag="OHO85_001323"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCQ2670724.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            48191..48364
                     /locus_tag="OHO85_001324"
     CDS             48191..48364
                     /locus_tag="OHO85_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670725.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            48454..49203
                     /locus_tag="OHO85_001325"
     CDS             48454..49203
                     /locus_tag="OHO85_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCQ2670726.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
BASE COUNT        11703 a        13117 c        12406 g        12170 t
ORIGIN      
        1 taaattaatg tatcagcaag tagtgctaaa cagcaccttt atacttcagc ctatctataa
       61 ataatgagaa tgccgagcca gcatgtcgac ggtgtggata gtacaggtga taaccgggta
      121 aatctggtgt gaatttatcc aaaacacgta tcagtttttt ttctttaata gcccgctcaa
      181 cctgatcata aggtagatac gccaatccat gcccatcaat tgcagcatca atgatcaggt
      241 ctatcgtatt cagtaaaagc tgaccttcca tgcgaacacg aacttcacgt ccaccgcgta
      301 ttaatctcca gcgatttgct gtacccgatg tgggaagata caaattaatt gcctgatgat
      361 ctattaattg tgacactgac gttggaacac ttcggcgaga aaaataatcc ggtgagccaa
      421 caatagccat tggtatatct ggcccgattc gaatggcgat catatcttta tccatctccc
      481 cacccagacg gacgcctgca tcaaaacgtt cagaaacgac atcggtcaaa ccataatcaa
      541 tggtgagctg aatatcaatt tcaggatgcg atttcaggaa tgtgcgcatt gctggtaaca
      601 atatcgtttt tgctgcatgt tctacagtag taatacgtat tgtcccggat gggcggttct
      661 gcagctcgct cagggatgcc agggctgaat ctatgtcatg caacataggg ccaagaacag
      721 acaaaagatg ctcgcccgct tcagttggaa caacgctgcg cgtggttcgc gtcagaagcc
      781 gcaatcccaa tcgttcttct atacgacgca ctatctggct taaagctgac tgcgccatgc
      841 tcaggcgggc tgctgcacga gtgaagctac gctcctctgc aacgacgaca aacgccatca
      901 gatcagcgat ttcttcacgc ttcattcagt atctccatgc aatctattaa tcatttaaat
      961 gataggtcta atcatgattt tgggtctaat caacttaatg atcaatatct atagtgatat
     1021 caccaaccgt taaaacgaac cgacaactcc atgaggaacg gaatgatgaa taataaagtc
     1081 agcttcacta acagcaataa tccaaccatc tctttgtctg cagtgatcta tttccctcca
     1141 aaatttgatg aaacccgtca gtatcaggca attgtggtat ctcacccagg tgggggcgtt
     1201 aaagaacaaa cggccggaac ctatgccaaa aaactggcgg aaaagggatt tgtcactatt
     1261 gcttatgacg catcttatca aggtgaaagt ggcggcgagc cgcgtcagtt agaaaaccca
     1321 tacattcgta ctgaagacat cagtgccgtg attgattacc tgacaacgct ttcttatgtc
     1381 gacaataccc gaattggtgc gatgggaatt tgtgccggtg caggatatac agccaatgct
     1441 gcaattcagg atagccgcat caaggccatc ggtaccgtta gtgcggtcaa tattggttcg
     1501 atgttccgta atggttggga aaacaatgtg aaatctattg atgccctgcc atacgttgaa
     1561 gcgggttcaa acgcccgaac cagcgatatt agcagcggag aatatgccgt tatgccactg
     1621 gcaccaatga aggagtccga tgcgccgaac gaagaactgc gtcaggcctg ggagtattac
     1681 cacacccctc gcgcgcagta tccaacagca ccgggttacg ctactttgcg cagccttaac
     1741 cagattatta cctatgatgc ttaccatatg gcggaagtgt acctgactca gccgatgcag
     1801 attgtggcgg gcagccaggc agggagcaaa tggatgagtg acgacctgta cgatcgagca
     1861 tcaagccagg ataaacgcta tcacattgtt gaaggcgcaa accatatgga tctttatgac
     1921 ggcaaagcct atgtggctga agcaatttca gtattagctc cgttctttga ggaaactctg
     1981 taatgaaaca tattcaaatc cgcaatagtg atatggcctg gcatattgct gccaataatc
     2041 tgggataatt ttcatgaaaa aggtactgat tcttggtgct ggcggtcaga tagcccgcca
     2101 tgtgattaat caactggcag ataagcaaac cattaaacaa actttatttg cccgtcagcc
     2161 agcaaaaatc cacaaaccct atccaactaa taaaatgcaa acaacttccg gcaaataaag
     2221 tgatacaaga tcgatgacat agcgttgaag aaaaggattt gataatccgc aaaatttgca
     2281 caacactgcc tgtaatactt cctgaaccag agacgatcat gaccgtaacc cgcccacgcg
     2341 ccgaacgcgg cgcatttccg cccggaacag aacattacgg acgttcatta ttgggcgcgc
     2401 cgttaatctg gtttcctgcc cctgcagcca gccgcgaaag tggtttgatt ctggctggca
     2461 ctcacggtga tgaaaactct tcggtcgtca cgctctcctg tgctctgcgg acgttgacac
     2521 cttctttgcg ccgccatcat gtggtgctgt gtgcgaatcc tgacggctgc cagctggggt
     2581 tacgggccaa tgctaatggt gtggatttaa acagaaactt tccggcggcg aactggaagg
     2641 aaggtgaaac ggtttatcgc tggaacagcg ccgctgaaga acgtgatgtc gtattactga
     2701 caggtgacaa acctggctca gaacctgaaa cccaggcgct gtgccagctt atacaccgca
     2761 ttcaacctgc ctgggtggtc tcattccacg atccactggc ctgtattgaa gatcccagac
     2821 acagcgaatt aggcgaatgg ctggcccagg cgtttgaatt gcctctggta accagcgtcg
     2881 gttatgaaac gcctggctct ttcggcagtt ggtgtgcaga tctcaacttg cattgcatca
     2941 ccgcagagtt tccgcccatc tcctccgacg aagccagcga aaaatatctc tttgcgatgg
     3001 caaacttgct gcgctggcat cctaaagatg caattcgccc gtcgtgaact gaagcgccgg
     3061 ttccacatct accgccagcc aggtcggtcc gtcaatatcg gcgaaactga cctgcggcac
     3121 cagcggtaaa gcggcgctaa tggcacgaga ggtacacaac atgcagccca gcatcagact
     3181 gaaaccttgt gcacgcgctt cagtcgccag cgccagcgct tccgtcagac caccggtttt
     3241 atcgagctta atgttaacca tctcatagcg ccctttcagc gccttcagat tgctacgtgt
     3301 atgacaactc tcatcagcac aaatcggcaa cggatgaata aaattctcca gcgccgcatc
     3361 gtcctgcgca ggaaggggtt gttcaagcat cgcaacgcct aaatccgcca atagctggca
     3421 acgcgccgcc aacccttctg cacgccagga ttcatttgca tcaacgatca gcgtcgcatc
     3481 gggcacagct gtgcgaattg ccaccatccg ctcactaata agatggttat ccagcttcac
     3541 tttcagtaat ttcgcgcctg cctgccagag tgttgatgca ctattggcca tctgatcagg
     3601 cgtaccgatg actaccgtct gtgcagtgat aactgtctcg ggaagcgtta tgccgatcaa
     3661 atcagccagc gattgctgct gttttcgcgc ggccagatcc cacaatgcac aatccagcgc
     3721 attacgtgct gcgccggcag ggagaatttt ttgcaactcc tcccgtgtca gccctttctc
     3781 taattgcgac acgacgctca taatttgcgc cattaccgag gcatcacttt ctccataacg
     3841 cggatacggc gtgcattcgc cggtgccttt aataccctct tcttccagtt caaccaccac
     3901 cacgcgcgct tcactgcgac ttccccgggc aatcacaaac ggggtatgta agggccaggc
     3961 ttcctcaaat accttaacgg ttctcatctt tactccttgt tgtcgcggtg gtctgcagat
     4021 taagcgcaaa tagcgtttgc tgtgttattg acagttagca taaactaggt gtgacgttaa
     4081 ctatatgtaa acaggaaaga taatcatgtc acaaaccgtt catttccagg gcaacccggt
     4141 tacagtcgcc aattccatcc cgcaggcggg tagcaaagcg cagactttta ctctcgtggc
     4201 aaaagatctg tctgacgtca ccctcggtca gtttgcgggt aaacgcaaag tgctgaacat
     4261 tttcccgagt attgataccg gtgtttgcgc cgcatcagta cgtaagttta accaactggc
     4321 aactgagatc gacaacaccg ttgtgctgtg tatctctgcc gatctgccgt tcgcccagtc
     4381 tcgtttctgc ggcgcagaag gtctgaacaa cgttatcacc ctctccactt tccgtaacgc
     4441 tgaatttctg caagcttacg gtgtggcaat tgctgatggc ccactgaaag gtctggcagc
     4501 gcgtgccgtt gtggttattg acgaaaatga caatgtgatt ttcagccagc tggtggatga
     4561 aatcaccacc gagccggatt acgaagcagc tctggctgta ctgaaagcat aatttaatat
     4621 gcctgatggt gttgcaccat caggcatatt cgcgcttact cttcgttctt cttctgactc
     4681 agaccatatt cccgcaactt attggcaatc gcggtatgtg aaacgccgag acgttttgcc
     4741 agtttgcgcg tgctgggata attgcgataa agctgggtta ataccgagcg ttcaaaacgg
     4801 ctggtgattt cgtccagcga accttccatc gcatcttcgc ccacggctac cgttgcggcg
     4861 tcataatccg gcaacaaaat atcctgtgga cgcagctcat aaccgtccag ttgtgtcagt
     4921 gcgcgataga tagcgttctt taactgccgc acatttcccg gccacgcata acgcgtaagt
     4981 acagtattca ggtcagcggc cagtttcgga cgcggcacgc cctgctcgtc ggcaaagcgg
     5041 gcgacgaaca gctcagttaa cggcatgatg tcctgcggac agtcacgtag cggcggcaga
     5101 ttgagcgtca acacgttcag acgataatag agatcttcac ggaacatgcc tttttgcacc
     5161 agttcgacca gattcttctg cgtagcgcaa atcacccgca catcgacatg cacctcatgg
     5221 tcttcgccaa cccgacggaa agtgccatca ttaaggaaac gcagtaattt cgcctgcatc
     5281 cgtggtgaca tttcccctat ttcatccaac agcaccgaac caccgttcgc ctgctcaaag
     5341 aatcctttct tcccttccgg agcatgacca aacagttcac tctcgaccgc atcttccggt
     5401 atagacgcac agttcagcgc caggtaaggt ttgcccgctc tggggcttgc ctgatggcag
     5461 gcgtaggcaa agagatcttt acctgtacct gtgtcacccg taatcagcag cggcgcgctt
     5521 agcatcgcca gtttctgcgc ctgttcgaca acatgcttca ttttcgggct gacggcgaca
     5581 atttgactga aggcgctgac gtcctgggcg gcgacatttt gcaactggcg gcccatacga
     5641 atcgttgatc gcaacatcac caccgcaccg gtcaggacgt gttgatcatt ttcatcctga
     5701 agataaacag gcgtaatctc catcaggaaa ttctgcccat taataacgac atgctcgtta
     5761 tgcgaatctt gcggttcgct ttccagccaa cgtaaaaaat taaagccgtt aatcaattgt
     5821 gcggcggtat ggttgcgcag gcgatccaat ttttgcccaa aaagctgaca gctcgccggg
     5881 ttcgccatat ccactttgct tttcatatcg acagagagca caggttcagg caacgcctcc
     5941 agtaacgcgc tcaacgccag atgctcacgt tcggaaggca tccacgggac agtacgcaca
     6001 tcggtaacac ccgcaatacg gcgtatttcg gccatcagac tgctgaaact ctcaaactcc
     6061 agttcagcaa aattgaggta gattcgccca atgggatcaa tctcaatacc gcgtaaatca
     6121 atgcctctta gcacgagtag atcgagtaat tcgcgggtca gaccgagtcg gtcttcacaa
     6181 aagacttcca gacgcatggg aaccttcacc tgaaaaaaga acaattaata tgatgatatg
     6241 acactatttg gatagcagga aggtttctgt caacaatcat tgacacaaag acggaaaaac
     6301 gggcacttcg gcgtaaagat atccggtcag cacccgatat tgcattattt ttcgctgggc
     6361 gttttgcctt tttgcagcgt ttctttcacc tgaccgataa gctgacgacg gaaatccccg
     6421 aggcgaggtt tgtcatcgtc aatccacggc agcgggcggc aaagctccat agctttaatc
     6481 ccgagtcgtg ccgtcagaag tcctgcacca atcccctgag ctgcgcgggt agacaaacga
     6541 gcagcgagat cttgcgacat ccagtccatc cccacttcgc gcaccagttc gctggctccg
     6601 gcaaaagcga tattcagcaa taccagctta aacagacgca aacggctgta ataccccagt
     6661 tcaatgccat acagcgtggc gatgcgatta attaaacgca gattgcgcca ggcgataaac
     6721 gccatatcga ccaacgccag cgggctgacc gcaatcatca acgttgattc cgccgccgaa
     6781 cggctgattt cgcgccgcgc ctgggcatct aaaactggct ggaccaaatg cgcatacagg
     6841 ctgaccactt cacggtcgtt ttgcgtttca tggattgagg cataccagcg ttgcagcgcc
     6901 ggatgcgact gatcaatacc cgcctgctgc gccagttttt cgcaaaatgc gcggcctttg
     6961 cccgtgccgt ggctatgcaa taaatcacgc gcttcgtcgc gttcatgggc gcgctgtcgc
     7021 aagcgccata agcgccgcca ctctgttacc acagaaccta cgccagcgcc gataatcaat
     7081 gccccagcgg cacatccacc cagcgccacc cagtcctggg tttgccaggc attcattgtc
     7141 cactgtacac cctgcccgac aacgcttgcg ccaaacagag ccagcccgcc catcaccatt
     7201 ttgcgccaca ggctgcgttt cggacgtaac gctgcgtcca ttaccgcttc gacttgcccc
     7261 tcttcttcct gcgcttcgtc gagcgtggcc ggggcaaaat tttgcgcctg attttcgtca
     7321 aaggtctgct gcgccctgaa tttaggattc tgatcgacct ccagaggacc gtcgaaatca
     7381 atacgtggtt ttaacggttc ggtcatcgca atttatctcc tattaaaaat tccagcgcag
     7441 catcaagacg aatatgcggt agtggtttgt cgacatccat cacctgcgga cgaaatgcct
     7501 caaactggaa gccttgctta tcccagaacg cctgaccagg caaacgtgcg ggaacttcgc
     7561 caggataaac agtgagcggt gcgccatcgc taagtcgatt accacgcagc gccgggattt
     7621 tctcaccgtt aacatcaata atgccgctgg tggtcgcctg aactgacgcc agccccaggc
     7681 agtccatgct gatcccttcg aacgccgcat tttgccaggc atcctgaatc agttgttgca
     7741 gtaatgaaac catattagcg tgctgatcga tggtcacatg gtccgcttta gtggcagcaa
     7801 acaatagctt atcgataacc ggcgaaaaca aacgccggaa cagggtacgc tgcccgtagt
     7861 gaaaactttg catcagctgc gtcagtgcca gacgcatatc attaaatgcc tgtggcccac
     7921 tgttgagagg ttgcaggcaa tccaccagca caatctggcg gtcaaagcgc agaaaatgat
     7981 tcttatagaa ccccttcacc accttctcgc agtaataatt aaagcgctcg cgcagcattc
     8041 cggcattggt atgcttatcg gcctgcgcca gtttggactc gccccaggta tcgacatccg
     8101 gccacgggaa gaattgcagc gcgggcgcac ctgccatatc tcccggcaag acaaagcgcc
     8161 caggctgaat aaagtgcagc ccctgctgtt tacagtggtg gagataatcg gtccacgcgg
     8221 cggcaatgtc cgccagccgg ttttcgtcgg caggcgctag cgggtcaagt ccttcgcaca
     8281 tcattcgcca tttcgccgac cattcgccgc gctgaccatt gagtaagccc gtcatctggc
     8341 gcgaccagct taagtaatcc tgcgccagca tcggcaggtc gagcaaccat tcgccgggat
     8401 aatccacaat ctccagatac agcgtggagg tgtccttgaa gtggcgtagc agcgaatcgt
     8461 tcgatttaaa acgtagcgcc aggcggattt cactgacgcc gcgcgttggc gtcggccagg
     8521 cgggaggatc gccatataac tgcgccagtc cttcatcata tgtgaagcgc ggaatgccaa
     8581 agtcacgctg aggaatgcgt tttacgccca gcaggcgctc ttcacgcacc gcgcttaaca
     8641 gcggcaagcg tgctccggcg tgaatattga gcaactgatt gaccatcgca gtgataaacg
     8701 ctgttttgcc gctgcggctt agtccggtta cggcgaggcg cagatgtctg tcgacacccc
     8761 gattcaccag cgcattaagt tcatttttaa gtcgcttcat tgctgtcctg tgctgctctg
     8821 aagaccagaa aaaaatcatt cgtaaggata tgaatgtggg ggccaggcca ttgattatca
     8881 actgcccgtg ttgtcatcga cgggcgaact ccataagatg tgcagcattc acgttcggac
     8941 ggagttcctg acctatgtcc cctactattt atgatattgc cagggttgca ggcgtatcaa
     9001 aatccaccgt atcacgcgtg ctgaataagc aaactaatat ctccccggaa gcgcgcgaaa
     9061 aagtgttacg ggccattgaa gaattacagt atcagccaaa caagctggcc cgcgcgctga
     9121 cctcttcggg ttttgatgcc attatggtga tttctacccg ttcgaccaaa actacggcgg
     9181 gtaatccgtt tttctctgaa gttttgcatg ccatcaccgc caaagctgaa gaggaaggtt
     9241 tcgacgtgat attgcagacg tcgcacaatc cggcagaaga cttacaaaaa tgtgaaagca
     9301 aaattaagca gaaaatgatt aaaggcatta ttatgctaag ttcgccagcg gatgagtcat
     9361 tttttgccca acttgataaa tacgatattc ctgtcgtggt gattggcaaa gttgaaggtc
     9421 aatatgccca tgtttattct gtcgataccg ataattttgg cgacagcatt gcgttgaccg
     9481 atgcattaat tgaaagtggg catcaaaata ttgcctgcct gcatgctccg cttgatgtcc
     9541 atgtttcagt ggatcgggta aatggttata agcagagcct ggctgcgcat aatattgcag
     9601 tacgtgatga atggattgtt gacggcggtt atacccatga aacagcactg caagccgcgc
     9661 gggaattatt aagccagtca ccgttgcctg aggcagtgtt tgctactgac agcctgaaat
     9721 taatgagtat ttatcgtgcg gcagcagaga aaaatattgc tattccgcag cagttagcgg
     9781 tggtgggtta tagtaatgaa acgctgtcgt ttattttaac gcctgcgcca ggcggtattg
     9841 atatcccaac acaggagtta ggtcaacaaa gctgtgagtt attattccgc ttaatttctg
     9901 gaaaaccgtc accacagaat attaccgttg ccacgcatat gacgctgaaa taaataccag
     9961 ctaccttgcc tgaatgccgg atgcgatgct agcgcatctt atccggccta catttatagt
    10021 tcggataaag tactcgtgcc gcatccggca atttagccca ttatcagaac gagtaattta
    10081 cgccgacacc tgcaaaatgg aatttatcgc tgtcgccttc gtcgtgatcc tgccactcaa
    10141 acgcgtattc cagcgaaacg gaaagcccgt tctggaaatc ataaccgtaa aataaaccta
    10201 cacggttaaa atcatggcct tcacgttcaa tatcatcctg ccagtcccag ttactccagc
    10261 gatccagccc aatgcgcgta tacggcgtca ccgagtggtt gccgagcgtc agcggcaaat
    10321 aggcgcgaat ttcttgcgta gaaaactcac cgttattgcg gctgtcgtcc atattaaagc
    10381 cgcgctcgag ataatagttc actcgcaagg caaccgtttc gttgaaggta tattgcaggc
    10441 ctgtttccgt ttcgactcgg gtatcagcgt aaccggtcgt gttcagatcg ttggcaaatt
    10501 tatacatcga taaccaaccg ttgaaacgta aatcgtccgt cagtttcaca tcccagtctg
    10561 gcgcgatttt ccagcgctgc atattcgccg tgtctttacc cggttcatca acgtagtgat
    10621 aaccataatt acggaaacca ccggtcaggc cgaaactgaa atcatcgttt tcgaggaact
    10681 gataatgcac ctccagctcc gggcgatcaa accacgttcc acgtttaccc gcgctataat
    10741 ctaccggccc ttcctgataa taggccagag caattctcca cggcccgttg gcggcattaa
    10801 aatagactga aggctccgcc agcccatcca tatcttcgcc ataaccctcg acgttttcta
    10861 tttcgtacat cgcgccgata ttaaagtgcc agtcgttctt ttcctcggcc tgtgcgcagg
    10921 ccattcccgc acacatcacc agtgcggtac agggtaataa ctttttcatt attgttcctt
    10981 gtattatcct gggggaatgc cttccccctg tatttagcga agtgctattt ccgtttctgc
    11041 atcaaagaaa tgacatttcg tcatatcaaa atgaatagtg atattttctc ctgcatgata
    11101 atcatttaac gcaccagcac ggaccactaa ctcgtgcccc ccaaccgtgg tgtagagcat
    11161 aaattccgca ccggttaatt ctgccacgct aattttggcg gaaatgttat tttcctcttg
    11221 cgcgtccgga tgaatatctt ccggacgtat tcccatcacg atgggcttat gcaaacttcc
    11281 ctgtgttttc agaaccgcta atttctcttc gggaatggtt aatttaagcg tttccgtaac
    11341 gaatttatcg ccatcgatcg tgccacgaat aaaattcatc gccggtgatc caataaatcc
    11401 ggcaacaaac atattcgctg gttggttata aaccgttttc ggcgcaccaa cttgctgaac
    11461 aatcccatcc ttcataatca caatccgcgt cgccatggtc atcgcttcgg tctgatcgtg
    11521 ggtcacgtag atcatggtgg tattcagctt ctggtgcagc ttgctgatct ctgcgcgcat
    11581 ttgcacgcgc agcttggcat caaggttaga gagcggttca tccattaaaa acacgcccgc
    11641 ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt tgcccgccgg aaagcgcgcc
    11701 cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc gccgcccagt tcacccgctc
    11761 atcaatcacc tctttggcga ttttttgcat cttcaggcca aacgccatgt tgtcgtaaac
    11821 cgtcatatgc ggatacaacg cgtagttctg gaacaccatc gctatattgc gtgctttggc
    11881 tggaacgtca ttcattcgtt tgccgtcgat caacagatcg ccgccgctga tctcctcaag
    11941 cccggcaatc atgcgcaggg tggtcgactt accgcagccc gacgggccga caaacacgat
    12001 gaactcttta tcggcaattt ccaggttgaa gtccttcacc acatgcacct ggttatcgta
    12061 gattttttga atatgttgta acgaaagctg agccatgttg attcctttgc tatacgtttt
    12121 gccagaaggc cgataaccgc ggccaggtga gtgattccgt tgaaggcaac agtaattgcg
    12181 ccccggttaa gcccgcgccg atccccaccg agcgcatacc gctggcgtta atggcgtcaa
    12241 tgcccgcctg cgcatcttca atgccgatac atgcctgcgg cggcacgccc agcccggcac
    12301 aggcggcgag aaagatttcc gggtccggtt tcgagttttt aagttgggaa gcatccgcgc
    12361 agaaggtgaa aaactcgcgc agctccagcg ccgctaaaat cgtcggcgca ttcagggaga
    12421 cagaagccag cccaaccggg atctgctgtg cacggagatc tgccagcaaa gagcgaatgc
    12481 cgggtagaac agcgctgacc gtcaactcgc gcagtgagtg gacatagagc agatttttgc
    12541 gatacgccag ttgcgccctc tcctgcgagt taaagtcgcc ctctttgccc ccgtgttgca
    12601 gaatgcgccg cagagactca tcgaggctga tcccttttag ggattcgtta aactgcgcat
    12661 caatgctgat gccaatttca gcggcaatct gctgccacgc ctggaaatgc agatgcgcgg
    12721 tatcggtgat tacaccatcc agatcgaaaa ttaccccttg cagtttcatt catcctcctg
    12781 atgtgtggta gcggtcccat tgaagggcaa aataaaatca cccaaacaga aaacagattc
    12841 ttcagccacg gatataagct gaccgttcaa acgcagtgaa acgggcgcag aagttcgaat
    12901 cgcaatacgc tgcgcgtcaa gagtgacctg taattcgcag ccctgccaga acagagggaa
    12961 agacaactgt tgccactgct caggtaacgc cggattgaga tgcaattcac cgtcacgcac
    13021 gctcaccccg gcaaaaccct gaatcgcccc cagccagata gcgccagttg cggcagcatg
    13081 gataccatca tcacaactat gcggatcagc accaagatcg atttcagtcc cctcgcgcca
    13141 gaactgataa ctttgggtca gcaggccaca gcgtgcggca acaatgccgt ggattgcttt
    13201 acttaatgac gagtcgtgaa tagtgcgcgg ttcataaaat tgcagattgg caagacacga
    13261 tgccgctgag aactgctccg gcagcatgta attgagcatc accacatcag cttgtttgag
    13321 gatttgcatc tcgttcactt ctgcgcgtga ataatccagc agaatggttt gcttccccgc
    13381 cgccgctttg tatttcgcca gattaatcgc cggcttagcc ataaacgaat catcctgcgg
    13441 caaaacgccg tcgggctgaa tttctggcag ccgcagttct ttaaggaaca tttcggcgcg
    13501 atggataaac gcatcgtcgc tacagccaaa ctggcgggca atactcagcg cctgctggac
    13561 gttgtaatac gccatatagc tggtgaaggc gttattattg acatgttcgg tatattcgtc
    13621 aggcccaata acatcatgaa tttccagacg gtcgttaacc ctcaccgcgc ggctaatcca
    13681 gaactttgca gtttccagaa gtagcgccat gccttcatgc gcaatgaaac tttcatcccc
    13741 cgtggtctgc cagtattgaa taaccgccca ggcgatatcg gccaccagat gatgttccgc
    13801 ctgcgccgag gccacttttt gccgcagccc ggtgcgaatg ttaatggcgg caaattccgg
    13861 cgtctcttct tcgccgctgc gcgcgctttc ccacggaaat agcgcgccct gccagccgtt
    13921 gcgtcgcgct ttctcctgcg cgcctggcaa gttgtgccag cgataacgca gtaaacttcg
    13981 ggcaaccgtc ggatcgctaa acagatgaaa cggtaacaaa aatacttctg tatcccagaa
    14041 aacgtggcct ttgtagcctt cgccggttaa gccttttgcc gcaatgctgc tgcgctcgtc
    14101 gtgggcaggc gtcatgatgc gcagatgata aagcgcataa tctaacgctt gctgatcgtg
    14161 cgcttcgccg ccatttaccg tgatacgacg tttctgccac cattgacgcc agttttctgt
    14221 tgatactgca agaagttggt cgtaactctg ctgcgcgcac atttcaagct ggcgaagcga
    14281 cgcgctgccc cactcgtcta aaacggcttg cctgtcatcc cgccagtcga tccacaccag
    14341 tttttgcaac gtcactgtct cgcctgcatg aagctgcgca ctggtatgtt gctgcaaacg
    14401 gcgctctttg gcggtatagc attgctgcac atcaccgctc accatacagc aacagctgat
    14461 ggccacatca ctgcgtccat cctgggtggt gtagatcccc tgcatcagat gctgaccaaa
    14521 cacccgcacc tgggtttcgt cgagatgttg gcgaccgtgg ttggtttgcg tggcgtcgat
    14581 acctgttgaa atcagcactg acgcgtcggc gtccagtggc gtaatagtga tttccagcac
    14641 aatgagcggc agttggtctg ccgaaacaaa gcgacggctg gcgatggtgt aacctgtgcc
    14701 gttgctggta cgccagacaa cgctacggcg taattctcca ctggcaaagt caagttcacg
    14761 ctgccaggct tcgcgggata acgagaaaac ctcaccattt atggcaatct ccatccccaa
    14821 gatatcaggc aggttcacca gttcgttgat ttcacctttt cccgcccgat gatacagccc
    14881 cgccagatac atccctcgcg tctggcgcgt gtaatcttct tcatggctgg cgcgaagccc
    14941 aagatagccg ttcccctgcg ccatcagcga tgcatacttg ttcagggtat gctggctgaa
    15001 atggggttct gataacgtta ctggcctggt cataattcca cacgcgtccc tgtttctgcg
    15061 gattgataca acgccgccac cagttgctgg atgatgtacc cctgctcggc gtcggctatc
    15121 atcacgggct tgccctgtac gtgattgata aaggcttcca tgctgcgcaa atggcggttg
    15181 tcgtctgcca gttcccgttg catcagcgtc attaattcac cgttgttatc ggtgtagata
    15241 tgtgctggaa acagcgtcgc accggcttta tcaccacaaa agctgacgtt cataatcgac
    15301 tgttcgcgga tgttgagtgc aaatgacgtt tccagccaca gaatgccgcc gttatgaaat
    15361 tcaatggtgc caaacagcga atcttcgacg ctataagttg ccggatccca ctcgccaaat
    15421 tgaccacagc tcttttgcgt gccgatcttt tgaaagctat gcgcattcac gcttttcacc
    15481 gccggaaagc ccagcacata catcgcagca tccagcatat gaatgccgat gtcgatcagc
    15541 gggccaccac cctgcagttc tttattggta aagacgcccc agccaggaac gccgcagcga
    15601 cgcagggcgc gggcggtggt aacgtaaatc tctcccaaaa cgccgttggt cacctggtca
    15661 cgcagctgtt gcgtatcgag cgcaaaacga tggtgaaagt cgtaggccag caccttgccc
    15721 agtttgcgcg cggtatcgca catttcccgc gcctgttctg gcgtcatggc gggcggtttt
    15781 tcgcacatca catggcagcc cgcttccagt gccatcaggg tatgttcgta atgaaaacgg
    15841 ttaggtgagc agacgctaac cacatcaggt ttcaccgcca gcagcatggc ctgcggatcg
    15901 tcccacacgg atgcattccc gtatttttct gccagcgcct gcgcctggga aaggcggctg
    15961 tcacagacag ccaccagttc cagatcgttg cgggtgcagt agtacgaagc atgaacctta
    16021 tccgccacct ggcctgcgcc tattatcgcg acccgcagcg gagagcttgt cattgcactt
    16081 ttcacaaaga cctcttagca ggtacgcaac caggccaacg aatcacggta cgcctgggca
    16141 ggatcttccg cccggatacg cccttcgtac actacatagc cctgatagtt atccgcgcgc
    16201 agctgttcaa acagcgcgtg gaaatccagg gtgccgctgc ccggctggta gcgatggtta
    16261 tccgcaatat gcacatgacc gagcaggtcg cggttgtcat gcagcgcctg cgccaggtta
    16321 tcttcttcga tattcatgtg atagaaatcg ccgataatct gtacatgttt aagatcgttt
    16381 tcgacgatgt aacggcgggc atcggcgagg gtgttgatca tatgatcctg atagcggttt
    16441 aacggttcga gatacaccac ggttccggta cgcgcggcga cctgttccag tacgcgcagg
    16501 gaatcactca ccattttgcg gtcgccgtcc aggctacgcg gcgaggtcat cggcggtaag
    16561 cggaaggtaa acatgcccca cgctgccgga acgacgatac ctttaccccc cacttctgcc
    16621 agcgcttcga gaatgcgttc gatctgcttt aagccattaa gacgacgctc ttcgataaag
    16681 tcgccaatcc aaccgtcata gccaccgcag gcggtggtca ccggtaaacc ggtttctttg
    16741 attgctgctt tgacttcttc gagattgttg accagcagtt tgccgtcaat ctcaaaacca
    16801 tcgaagccca tctctttgat ataacgaaat ttctccagaa tgttttccgg aaagaatgcc
    16861 tgattctgtg tgcctatttt cattttttca ttcctttgaa ttaaaacgta acgcccattt
    16921 tgatgctctg ttccggatgc tgatcgacat actgcatgta gctttccggg ctattggcaa
    16981 aggtcactac cgggtcgatt aaatcttcgc aattgagata accgttcatc agcagttccc
    17041 agcaggtttc ttcaatacgc ttacggctcc agcgcggata atccgggttc ggttcgctgc
    17101 acgcgcggga gaaaacaatt ttggcgttat tgaaatgcgc ttcgcgtccg aggttaaaac
    17161 cttcagcaaa tggtttggca aacgcgacat aagagatggt gccgccataa gcaaggccac
    17221 gcagcgccga ttgcagcgcg tcggcgtaac cgctggtttc gataatcaca tcggcaccct
    17281 gcttgccggt cagcgttttg atctcttttc ctacgtcagt gccaatgggg ttaaggcaga
    17341 aatcagcacc gtggcgacgg gcaatatcac agcgataggc gataggatcg acgccaatca
    17401 ccaccgacgc gccagcgcgt ttcgccagtt ggatggctat ttgaccaatc gcgccaagcc
    17461 ctaccaccac cacaaaatca cccacgcgca cgttggcatc gcgcacgccg ctcatggcaa
    17521 actgcgccgg gtcgtaacaa acggcgtttt tccacgagct gccttgcggc attttgcgca
    17581 gcttgtagtt attcaccgcg ttaatgatga ccgtctcgga gagcgggccg tagccgcata
    17641 cgctgtcgcc caccgcgtag tcggtaacgt cgctgccgca ctcgataatg tcgccaacca
    17701 ccatgttgcc aagctggaat ttgccaaact caatgccgcg cggcgcatct gccggacgcg
    17761 gagtgaacat ctgccattcg ccgttaaagt cttcatcgat aaacgggctg gcggcgcgga
    17821 agtcgaccac ttccgttccg tgtttcggtg cgccgaaacg cacgcggatt ttcacttcat
    17881 tggctaaaat cgcccgatct tcatactcaa ccagcgcagc aacacgcggt gctgtggcta
    17941 ctaacttttt catttaaaac tccttgtaaa gaaactggcc gctttagccc ttcacgccac
    18001 cggcggtcaa accactctta ataaaacgtt ccgaaagcgc atacataatc accaccggca
    18061 atgcggtcac cagtgaggcc gccatcatcc gtccccagat gtaatctggc gtactgaaca
    18121 gcgcgttcag gcccaccggt aaggtgaaat tgctggcgct ggagaggaag atcgacgcaa
    18181 acagatagtc gttccacgcc accataaagc agtagacgaa cacggatatc agcccggaca
    18241 tcgccaacgg cacggtgatg cggaagataa tttgcagacg gttgaggcca tccatcatcg
    18301 ccgcttcttc gatctcatcg gggatggtgt cgaagtagct tttcagcata aacacggcgg
    18361 tgggcagagt ttgcgtcacc atggtgatga tcagcgccat ctcagtgtcg taaatgccca
    18421 gcgcggtgat gattttgaac agcggcacca ccagcaaaat gccagagaac atatacaccg
    18481 tgtaaaagct ggcgttgatg gtcatccgac ctttaaagcg caggcgggaa agcgcgtaag
    18541 ccccaagaat gccgaggaat accgccacca cggatgaaac cacagacacc accagactgt
    18601 tacggaagta gtcgacaaac gggaaaatca tcgggttaaa aatgtcgacg taatgctcca
    18661 gcgtccactg ctgcggcagc agcgtaggat gcagtgagat cgcctctttc gcgcccttga
    18721 acgaggtcat cagcatcaca aaaaaaggaa acagcgtgat gatgagaaac agcgccagcc
    18781 cgcagtaaaa accgatgcga ctgagtgtgc gtttatttgt tgccattgag gttcacccgt
    18841 ttcctggtca gcagaatgac agcgaaaata atgatgaaga gcaccaccga aatcgccgcc
    18901 gctttcccta agtcattaaa ggcaaaggcg gttttgtaga gatagacgcc gagaatatcg
    18961 accttggtgg tcagcaaata aacatccgcg aacatgtaga acatccagat ggtgcgcagg
    19021 gtcaccaccg tcgccaggac cggcataatc gcgggcagcg tgacgatacg aaaccgttgc
    19081 caggcattag cgccatccat ctctgccgct tcgtacagcg atttgtcgat ggtctgcaaa
    19141 atcgccagaa acgagataaa ggcatacggg aagtagcgcc agatggcgaa cagcaccacc
    19201 agcacaaagc tactgcccgg attatcgaac cacagcggtg cctgctcata gagatgcaga
    19261 agatcgacgc cgaggtagtt cacaatgccg tagccgttgt tgaacatgta tttccaggcg
    19321 aacaccagcg aaatggacgg cgttacgtag gagaggatca ccagcgaacg cgcggttttg
    19381 cgcaggcgga actcgcgatt gaaaaacatc gccaccgcca gcccgagcac ggtgctgccc
    19441 gccaccacca gcgcggtgta ccagaccgtc atccacagtg agtgccagaa gccgggatcg
    19501 gagaggatac gcacatagtt gctcacccca acaaaggttg actcgatgtt gggattgagc
    19561 ggcagacgta aaaaactaat ttcgatattc gacaccatcg gccacgccac cagaccgccc
    19621 agcagtaata agctgggcgc gagaagcaac agcgcaaagg gcatatcgga acgacctgaa
    19681 aaaagcctgt tcattgcgga ttcctgtcct gggtttagtg ctgttcgacc agttcatcca
    19741 gctttttctg gctcgcttgc agcgtgccag ggagatcggc tttacccacg gtgacgttat
    19801 gcaccatgga gctcaccacg ccagaccccg tcacatcacc catgcgggta aagtttttat
    19861 cccctactgc accaaagacc tgaatatttg gcagttcacc gattagctga ttcggtagtt
    19921 cacccagcgc cttaataacg tcgttgtctt tccaggtggc ggtagtgaca accgctttat
    19981 tcaccggcag cgcagcacct ggcgacatca tcacccagtc ggcaatgttg tctgcctgct
    20041 ccataaaggt gacaaatttt tctgctgctt ccgtctcttc ggtcttttgc ccggcggtaa
    20101 tggtcagcga ggtcaacatg ccgtagaccg cagagttttt ctcggttggc accacgaaac
    20161 cgacgttttt cgggtcgcct tctttaatca cagccggaag gatataggtg gagtaaatcg
    20221 ccatcggcgc ggtgccgttc ataaaggcgt ctttcacttc catgatgtcg ttagaacccg
    20281 gcatagtgtt ggcagcaagg tcgcggtaat aggtcagtgc ctgcatcatc tctggtgtat
    20341 caagggtgat tttgccttcg gcgttaaaga cgttagcctg gttggataac gcaaactggg
    20401 agaaggattg ttccgtcaac acgctttctg ctgtaggcag cgcaatgccg tattttttat
    20461 tcgccgggtc attcagtttc tgtgcaacgt ccagcagctg ttgccagttt ttcggctcct
    20521 caagccccgc ttttgccagc acatctttgc gataccagat accgccaatc caggcgctga
    20581 caggaacacc ggtccatgcg ctaccatctt cggtacgcac aatacgcagt acgccatcgt
    20641 aaaacgcgcc ttcaccaacg ttgctgatga ctgtggcaac cgctttgcga tcgataagct
    20701 gctctttgtc catcactttg gcgtagtcat ggctggtttc gatcacttcc ggcagcgaac
    20761 cgctacgtga aagagtaatg actttagtgt tataggcatc ttcttccacg ggcacttgct
    20821 taacggtgat gccagggttt tctttttcaa aacgggcaat caatttactg ataacggcct
    20881 ggcgctcctg ctccaccgaa gaatgcataa attcgatgga tacattcgtt ttattttctt
    20941 ctttacagcc tgaaatcagg gcgcaggaaa ccagtgctga taacagcacg atttttgatt
    21001 taatcatgag atgttccttt ttatttaatc cacataacct gccaggggcg cagcgttatt
    21061 tcattaccca atatatcaac ttcactaatc agatcccgac catgcagatt agtaatattt
    21121 acatgctgaa tatttttact gacattaaac aatccagtca gacaattacc atcagcgtta
    21181 cttctttgaa tacgcattac ggatgaatta atcgtatcaa tggtaaaatt attatccgga
    21241 tgaaactcgt tatggctgcg acgaagtgta attaaacgcg acaactcatg atataccgca
    21301 tgccttaatg tagcttcatc gttcagttct cgggttatct ctttactgtg atatttttta
    21361 cggttaatcg cacggttata tccgagtttt tcgacacctg cgtaatcatt acgcgagcca
    21421 agaatacttt gaatatatat cgctggcaca ccggggaaac ttaacaaaat cgcatgggcg
    21481 agaataaacc tggcgcaacg ttcttcatcg ctactctcac ggcggcttaa cgcatccata
    21541 taggtcacat ttatttcata cggactgcgt gtaccgtcgg gattattttt ccagtttact
    21601 aatgcacctt cctgctgtaa cgcctcgacc agctctaata tttcgctttc aggcaacaag
    21661 ccccgtagcg ggtttagccc gatgccatcg tgagaggcga ggaagttaaa ccaggtggtg
    21721 ttgctggaag gtagtgtcag gttttgcgcc catgcacaaa gcgcctcaac gttctgtttt
    21781 tgcaccgcat gcagcaccag tggcggcagc gagaactggt acaccatatg tgcttcgtca
    21841 tcgcctgcgc caaagtaagc aatgttgtct ttatgcggaa cattggtctc ggtaatgatc
    21901 actgtacctg gcgcaacgtt atcaataatc gaccgtaaca gtttgataat cagatgtgtt
    21961 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
    22021 cggacatact cagcaccttt cgcaaggtaa cagagtaaaa catccaccat cgccagcaac
    22081 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
    22141 cgcgttgaat gatcgcgcat ctggaatggc gttaataacg gtaacgcacg cgggcgagtg
    22201 acggcgctga gatcggtttg cgggtcaacg gcaataaaaa aatcttcaaa acctggatgc
    22261 tgttgtaaat agtttttaaa ccattcactt tttgccgaca tatggttgca gacaaaatca
    22321 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
    22381 gcgacctgtt gataatcaat taccgaaaag ccatcatcag atgaccatgg ataaaacggc
    22441 aacaaataaa catgtgaaaa aatgctttgc agccattgat ggtaaaactg attaaatgtg
    22501 ggtaatggtt ttaaatcatt gctgtgaaat tgatcggcat aggtaattaa cacgacatca
    22561 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
    22621 gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccaggtga
    22681 tccgtaattt tctgtttcat gttgcctttc cgtggtagcg gtcccatgaa acgagagact
    22741 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
    22801 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatggg gatgtaaata
    22861 aggtgtgaca gaaaaaaaaa cggcgcataa gcgccgctca tggtgaattc ttttaacctt
    22921 tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg gtatatccca
    22981 tctcgctaag gatctctttt gcttgccctg actggcgtcc ggcattgcaa tacactttca
    23041 cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc ttcaggggaa
    23101 tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga acatcgatcc
    23161 agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt aagccttttt
    23221 taaacatgta agcgtccttc tatggatgaa aattgccatc cattataggc ctgagattta
    23281 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
    23341 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
    23401 ttttatcgcc cagcccgcta cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
    23461 cttgcctgct aatttgaaac caggctttac cttttgcccg gcctgttgcc agcgagtatt
    23521 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
    23581 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
    23641 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
    23701 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaagaaaatc
    23761 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaaccccg
    23821 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataatttttt attgagatta
    23881 atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaatg gattccagcg
    23941 cctgaatacg ttcgcgcatc cgttttgctt catcagccag ttgcgctaat cgctgctgct
    24001 cactttgcga caattcactg cgaccagaac gattgctgta atgtagccat aaccagatcg
    24061 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
    24121 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
    24181 ttgcttcatt ttggctttta attgtgccag ttgctcgctt attgcatcat cggctttcag
    24241 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
    24301 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
    24361 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat ggcgtaacat
    24421 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc cgatctcttt
    24481 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccagtg acttaatcag
    24541 atcggtcagt ttctgttttt caattaacgc agcacgcgcc aggtcttctc tatctttcag
    24601 cagtgccagt tcggcttttt cctgccattc aacttcacga gccgacgctt gttcaatgcg
    24661 gcgagtaagc tgtttctttt ccgccagcgc acgcgccgaa gtagaacgca cttcaaccag
    24721 cgtgtcttcc atttcctgga tcatcagacg caccagtttc tgcggatctt ccgctttctc
    24781 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
    24841 gttgtcctct tgatttctgc attcaggatt gtcctgctga actgttaata caatttgcgt
    24901 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
    24961 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggcag aatacaaaga
    25021 taatttactc ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
    25081 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agctgatcgc
    25141 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
    25201 ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
    25261 cggtgcgcaa aaacgtcatc caggaagatt tgaacgtgcc gacggcggta cgctatttct
    25321 tgatgaactc gctacagcac cgatgatggt gcaggaaaaa ttattgcgcg tgattgagta
    25381 cggtgaactg gagcgcgttg gcggcagcca gccgttgcag gtgaatgtgc ggttggtatg
    25441 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
    25501 cgaccgactg gcttttgatg ttgtacaact gccgccacta cgcgaacgtg aaagcgacat
    25561 tatgttaatg gcggaacact ttgccatcca gatgtgtcgg gaaatcaagc tgcctctgtt
    25621 cccgggtttt accgagcacg ccagagaaac attgctgaat tatcgctggc cgggaaatat
    25681 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
    25741 gcttgatgac atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt
    25801 ttcagaaaac acttcgcttc caacactgcc gctggattta cgtgagtttc agatgcagca
    25861 ggaaaaagag ttgctgcaac tcagtttgca acaagggaaa tataaccaga agcgcgcagc
    25921 tgaattgctg ggcttaacct atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
    25981 gcgaagttgg gcaatacaac ttgccggatg cggcgtgaac gccttatccg gcatacataa
    26041 tcaatacgtc gtttgcaggt ctgataagcg cagcatatca ggcattattg cgttgcccct
    26101 gcttctgcgt attaatctct cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc
    26161 gcatccggga tggtcagcgg atacaggaaa cgaatcacgt tgccgtatgc gccacaggtc
    26221 agtagaagca gtccctgcgc cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac
    26281 ggctcgcccg tttgcggatc gttaaactct gccgcaatca tagaccccag accgcgtacc
    26341 gccgcgatgg ccggaacgct ttctttggca tcaatcagcg tgtttgtcag acgctggccc
    26401 agttgattcg cgcgttcgca gagtgattct ttgtcgataa tgttgagcac cgcgtgcgcg
    26461 gcagccaccg ccagcgggtt accggcgtag gtgccgccaa gcccgcccgg cgcgggtgcg
    26521 tccataatat tcgcgttacc gaccacgccc gaaagcggca tcccgcccgc gaggcttttc
    26581 gccatcgtca ttaaatccgg cttatcggcg taatgatcca tggcaaacag cttaccggta
    26641 cgcgcaaagc cgctttgcac ttcatcagca atcatcacaa taccgtgctc gtcgcacagg
    26701 cggcgaatgg cggcaaccag ctcttttggc gcaacgttga aaccgccctc gccctgcacc
    26761 ggttcgaaaa taatggccgc gacctgcttc gcttcgatgt ctgatttaaa caagcgttcg
    26821 atggcgtcga gggagtcctg tgttgaaatg ccgtgtaaat ctgacggata aggtacgtga
    26881 tacaccgaac cagggaacgg gccgaagccg attttgtacg gcgcgacctt tccagttaac
    26941 gccatagtca tatacgtacg accgtggaag ccgccgctaa acgcaatcac gccagggcgt
    27001 ccggtatggg cgcgggcgat tttcaccgcg ttttccaccg cttccgcacc ggtggtgaag
    27061 aacgcggttt tggcctgccc gctcaccggg gcaagggcgt tgattttctc cgccagggtg
    27121 acgtagcttt catatggcac aatctgatac gcggtgtggg taaactgttg cagctgctgc
    27181 tccaccgccg cgaccagatc agggtggcga tgtccggtat tcagcaccgc aatgcctgcg
    27241 gcgaaatcga tgtactcgtt gccctcaaca tccttcagcg tggcgttttc agccgactgg
    27301 gcgaagaagt tacacatcac gccaaccccg cgcggggtgg cagaaagacg acgctgatgg
    27361 aattcattgt tgctcatgtt tcacaccttt tgactcttca ctgttaatcg gaaattaaac
    27421 gcccagacga tcgcgcaggc tgtaatacgc cgcgcccatc gcggtaaacg gtacgcgcag
    27481 cgtgcgcccg ccggggaacg ggtaatgcgg cagattggcg aaggcatcga aacgttcggc
    27541 gtcgccgcgc agcagttcgg caatcagacg cccggcgagg tgggtacagg tgacgccgtg
    27601 gccgctgtag ccctgcatgt aatagatgtt ggtatcgagg cgaccaaact gcggcatacg
    27661 cgacagggtc agcaggaagt tgcccgtcca gcggtaatca attttcacgc ccttcagctg
    27721 cgggaaggtt ttcagcattt tcggcaccac aaggcgctca acatcgtccg ggtcgcgagc
    27781 gccatacact acgccgccgc cgtacagcag gcggttatcg gcggtaagac ggtagtaatc
    27841 aagcagatag ttacagtctt cgacgcagta gtttttcggg ataagggaac gggctaaatc
    27901 ttccgacagc cgctcggtgg tgatcacctg cgtgccgcac ggcatgctgc gtttcgccag
    27961 ctccggctcc actttatcgc ccaggtacgc attcccggcg acaatcacat acttcgccgt
    28021 cacctgacct gtggctgtac gcactacggc gggcgtggtg tgctgaatct gcgtcactgc
    28081 ggaaagctca tacacgcgcc cgccgttcag gcggatagcg tccgcttcgc caatggcgag
    28141 gttcaacggg tgaatatgtc cgccgctgtg atccaacagc gcgccggtgt agcggtcgct
    28201 ggccacttca cggcgaatgg cgttggcgtc cagcagttcc agttgtttat tgccgtagcg
    28261 ttcccagttc tctttctgct cttcgagtgt ggcgagttgt ttatcattca tcgccacaaa
    28321 cagaccgccg gggcggtagt cgcagtcaat ctgataacgt ttgatacgtt cgcggatgat
    28381 ctcaccacct tcgaacatca tgctgccgag catacgggcg gtgtccatgc cgtagctttt
    28441 ttcgatcaca tcgatgtcgc ggctgtagga gttcacaagc tgcccgccgt tgcgtccgct
    28501 ggcgccaaag ccgatgcgtg aggcttcgag aaccactacg tcaaagcccg cttctgccag
    28561 atgcagtgcg gaggaaagcc cggtatagcc gccgccgacc acgcaaacgt cgcaggaaat
    28621 cgactcattc agcgtgtcga atggcgcgta tttattcgca ctggcggcgt agtaactgct
    28681 ggtatgttcg gtcatttcag gcctccaggc ttatccagat ggttttcagt tcggtgaatt
    28741 tttcaagggc atgcagggat ttgtcgcgac cgttgccgct ctgcttatag ccgccaaacg
    28801 gcacggtcat atcgccgtcg ttgtagttat tgacgaagac ggaaccggct ttcaggcgtc
    28861 ggctcatgcg gtgcgcgcgg gagaggtcgc gcgtccatac cgccgcgcca aggccgtact
    28921 ggctgtcgtt ggcaagctgt agcgcctgtt cttctgatgt gaaacgcgtg accaccagca
    28981 ccggaccgaa aatctcttcg cggcttaagg acgcattcgg atctacatcc acaaagatgg
    29041 tcgggccgat ggcggcagcc agcccggcgt tacggccatc caacaacagt tgccctttgc
    29101 tttcgccttc ccgaataaag ctatggaccg agtcggcgtg ggcgcagtcg attaaggtgc
    29161 ccatggtggt tgcgggatca agtggatggc ccggctgcca gttttgcgcc tgctgtttta
    29221 acagggctaa gaattcatcg gcgatgctct cttccagcaa caggcgcgtt ccggcgatgc
    29281 acacctgacc ctggttgtag aagatgcctg ctgcggtggc gcttgccgcc tgttgcaaat
    29341 ccgggcagtc agcgaaaacg atgttggcgc ttttgccgcc cgcttccagc cagacgcgtt
    29401 tcatgttgct gtcgcccgca tctttcagca gctgtttccc ggtacgggtt gaaccggtaa
    29461 aggcgatggc atcgatatcg ttatgacgcg aaagcgcctg ccccgcttca tgaccaaaac
    29521 ccgttactac gttcagtacg ccatccggca gacctgcttc tttcgccagc ccggcgagac
    29581 gaatcgcact gagcggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca
    29641 gcgccgggcc gagtttccag caagtcagca gcagcgggaa gttccacggc acgatggcgg
    29701 caattacgcc gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca
    29761 cttcgccata cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg
    29821 gaatatcatc acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg
    29881 ccagctcttc ggcgtgggct tccattaaat cggcgagttt attcagtaca gctttacgtt
    29941 tagccggaga agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg
    30001 cacggtcgat atcgacgctc ttgccgcggg caattttggc cagcggtgcc tgggtgaccg
    30061 gatcaacggt ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata
    30121 agcgggtttc aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat
    30181 tcattatgac tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcggggcgca
    30241 gagatcccca ggccgtatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg
    30301 cattgcgctt acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct
    30361 gataattcgg caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat
    30421 ggcataactt tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc
    30481 accttccagt actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt
    30541 gcccggctgg tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac
    30601 cagcttcatt gacacaccct gactgcccat ctcaattaag tcgtcctgat taatcacgac
    30661 ctgcggctca tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac
    30721 cttcagcagc ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact
    30781 gatagcactg tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg
    30841 ctggcggatt tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt
    30901 tccttaaact gtagtggtca gagtcgctgt ttttcagcga tatggtgctg acaagcggtg
    30961 ataaagccct cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc
    31021 cactgtacgc ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc
    31081 ggcgagcgag cttcaacacg caaccgtggg ctaacgacct tcgccccttg cccatgcaga
    31141 gagtttaccc aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca
    31201 acctgaactt catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga
    31261 tgttccagca attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc
    31321 accagttcct gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca
    31381 ttaattaacg ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt
    31441 tcaccatata ggtgcggctg cacattgctg ggactaccag gaagataaat gccatcgagt
    31501 ttcggcaaaa gttgttcaag taatgacggt tccgccagcg catgtggtag cgcaattggc
    31561 aagccgcctg cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga
    31621 cccttaagcc tgttcctgca cattacgaca ccgacaaccg gattgttcat tatattttcc
    31681 atgctcaatc tcacaaagtg gactaaatta tcgccattac tgcggtttaa atgaccttta
    31741 tgttcaatat tttttcaatc tagcagtggg ttttcatttt tgcaaactca attaacattt
    31801 gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta cgctttgata
    31861 acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga
    31921 actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct
    31981 acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt
    32041 gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gttggagaaa gggtgttatt
    32101 tcccggcctc ggtgtttgca atggatattc tgggcaacgt agtagaagaa gcgggtctgg
    32161 gtcaggaaat gggcgagccg gatcgtactt gtgttcctgt tctcggttcc ttaacccctt
    32221 ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg
    32281 ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctacgccagc
    32341 gcggattgtt cccggttgta gcggtagagc tggagttcta tttactggat cgtcagcgcg
    32401 acgctgaagg gtatctgcaa ccgccctgcg cgcccggcac cgatgaccgc aatacacaaa
    32461 gccaggttta ctccgttgat aacctcaacc acttcgccga tgtgctcaat gatattgatg
    32521 aactggcgca gttacagtta attcccgccg acggtgcggt cgccgaagcc tcgccgggtc
    32581 agtttgaaat caacctttac cataccgata acgtgctgga agcctgcgat gatgcgctgg
    32641 cgctaaaacg cctggtgcgt ctgatggcag aaaagcataa gatgcacgcc acttttatgg
    32701 cgaagccgta tgaagagcac gcgggcagcg gaatgcatat ccatatcagt atgcaaaata
    32761 accgtggcga gaacgtgctt tctgacgcgg aaggcgaaga ttcgccgctg ctgaaaaaga
    32821 tgctcgccgg gatgattgac ctgatgccgt cgtcgatggc gttgctggca ccaaacgtga
    32881 actcgtatcg ccgcttccag ccgggaatgt atgtgccgac gcaggcgtcg tggggccata
    32941 acaaccgcac cgtcgccctg cgtattccgt gcggcgaccg tcataatcac cgcgtggaat
    33001 atcgcgtggc gggtgccgat gccaacccat atctggtgat ggcagcgatt tttgccggta
    33061 ttttgcatgg ccttgataac gagctgccgt tgcaggaaga agtcgaaggc aacgggctgg
    33121 aacaggaagg cttacccttc ccgattcgcc agagcgatgc cctgggtgag tttatcgaga
    33181 atgatcacct gcgccgctat ttaggcgaac ggttttgcca tgtgtatcac gcctgtaaaa
    33241 atgatgagct gttgcagttt gagcgcctca tcacagaaac agaaattgag tggatgttga
    33301 aaaacgcgta ataccccttg cggggttgtt tcatcggcct gagcggagcc aggcctgaca
    33361 tttgctggta atccagacat ttcccggatg tcgcgttatg gagcgcgggc ggcaacgggc
    33421 ttccgttaac gattaagact gtgacgagga aatgagatga tgccgatgat taaatccccg
    33481 catggcgagg gaggctgcgt atgcgcccct cctgcgactg actggactcc gccccctttg
    33541 cttcccttgc taaacaggtt tgatttcagg tcgacacgac cgcaaacctt attacgcagg
    33601 ggaggcagca attatggcta ttaattcacc actgaatatt gctgcgcaac ccggcaaaac
    33661 ccgtctgcga aaatcactga aattgtggca ggtggtaatg atgggtctgg cctatctcac
    33721 gccgatgacc gtatttgata cttttggtat tgtctccggc attagcgacg gtcacgttcc
    33781 agcgtcctat ttgctggcgc tggcgggcgt gctgtttacc gctatcagct acggcaaact
    33841 ggttcgccag tttccggagg ccggttcggc ctatacctac gcgcaaaagt cgattaatcc
    33901 gcacgtcgga tttatggtcg gctggtcatc actgctggat tatctctttt tgccaatgat
    33961 caacgttttg ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc cgccgtgggt
    34021 gtaggtggta accttcgtcg ccattttaac cgccgcgaat ctgaagagcg tcaacctggt
    34081 cgctaacttc aataccctgt ttgtactggt gcaaatctcc atcatggtgg tgtttatctt
    34141 cctggtggtt cagggactgc ataaaggaga aggcgttggc accgtctggt cacttcagcc
    34201 gtttatcagc gagaacgcgc acctgatccc gattattacc ggtgcgacga ttgtctgttt
    34261 ctcgttcctc ggtttcgatg cggtgaccac gctttcggaa gagacgccgg acgccgcacg
    34321 cgtgatcccg aaagccatct tcctgacggc ggtatacggc ggcgttatct ttatcgcggc
    34381 gtcgttcttt atgcagctgt tctttcccga tatcagccgc tttaaagacc cggacgccgc
    34441 actgcctgaa attgcgctgt acgtcggcgg caagctcttt cagtcgattt tcctctgcac
    34501 cacgtttgtg aacacattag cgtctggcct ggcttcacat gccagcgtgt cgcgtctgct
    34561 gtatgtgatg gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg tgcacccaaa
    34621 atggcggact ccggcactca acgtcattat ggtcgggatt gtcgcgctgt cggcgctgtt
    34681 ctttgattta gtcaccgcga cagcactgat taacttcggt gctctggtgg cgtttacctt
    34741 cgtgaatctg tcggtgttta atcatttctg gcggcgtaaa ggaatgaata aaagctggaa
    34801 ggatcacttc cactatttgc tgatgccgct ggttggcgcg ctgacggtgg gtgtgctgtg
    34861 ggttaaccta gagtcaacgt cactgacact cggtctggta tgggcttcgt taggcggcgc
    34921 atatttgtgg tatctgatcc gccgctatcg caaagtgccg ctgtacgaag gtgacagaac
    34981 gccggtgagc gaaacgtaat agccggatgc gcggcgcatt atcctgcctg agggtaacgc
    35041 gccgcgccat taactataaa aagatgtatg acccggatca cgagtgctat catccgccct
    35101 tatgcttagc gcattaaccc caggggcaaa taatgaacca cgatatcccg ctaaaatatt
    35161 ttgatattgc cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagag gctgaacgca
    35221 caccgctggc ccactacttc cagttactgc tcacccgttt gatgaacaat gaagagatca
    35281 gcgaagaagc ccaacatgaa atggctgctg aagcggggat taatcccgta cgcattgatg
    35341 agatcgcaga gtttttgaat caatggggta atgagtaatt atttaattgc agacgatcgg
    35401 tcagtatcgt tgtccggata aagcgttcac gccgcatccg gctaacaatg acacgcactg
    35461 cagcgtgctt taatgggttt ctcacgatcg taccgctctg ctcctcgcta agttgttctg
    35521 tgagttatag ttatagagaa atcacgggta cgaatgttga acagatggcg acgccacctg
    35581 ttcaacgacc aaccacacgg ccctcattac gacccctgaa cacaaacggt aatggcaaat
    35641 gtcattaaat attagtaaga tagcctcttt acataaaatc ccttaatatt atgcgccagg
    35701 tattatcgtc tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg
    35761 aatccccccc gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag
    35821 tcaacacctt taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc
    35881 agttttatga tcgactgctg gatgtcgatc cctataccta tcgcctgatg ccggaacttg
    35941 ccgaaagctg ggaagtactc gacaacggcg cgacctatcg cttccacctg cgtcgcgatg
    36001 ttccgtttca aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg
    36061 tgtttacctt ccagcgaatt tttgaccgca acaacccgtg gcataacgtc aacggcagca
    36121 acttccccta cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc cgcaaactgg
    36181 ataatcatac cgttgagttc cgactcgccc agccggatgc ttcttttttg tggcacctcg
    36241 caacccatta tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc
    36301 gccaggagca actcgaccgt caaccggtcg gcaccggtcc gtatcaattg tcggaatacc
    36361 gcgccgggca atttattcgc ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa
    36421 tgccgcaggt ggtggtggat ttaggctccg gcggcaccgg acgtctgtcg aaactcctga
    36481 ctggtgaatg cgacgttctg gcctggcctg ctgccagcca gctatccatt ttgcgtgacg
    36541 acccgcgctt gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca
    36601 ccgccaaacc gccgctaaat aatcccgctg tccgccatgc gctggcactg gcgattaata
    36661 accagcgcct gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac
    36721 cgcgcgcctc gtgggcctat gacaacgagg ctaaaattac tgaatacgat ccggcgaaat
    36781 cgcgcgaaca gttgaagtcg ttggggctgg aaaatttaac gctgaaactg tgggtgccca
    36841 cacgttcgca ggcgtggaac cccagtccac tgaaaactgc cgaactgatt caggcggata
    36901 tggcgcaggt tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt caggaggcgc
    36961 ggttgatgga tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc
    37021 cggacagttt cttccgtcct ttactgagct gcgcggcaat tcattcacag accaacctcg
    37081 cccactggtg cgatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc
    37141 tggcagcgcg tattgaagcc tatgacgaag cgcagagtat tctggcgcag gaattgccca
    37201 ttttgccgct ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg
    37261 tacttagccc gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa caggatgagg
    37321 tgaaaaagcc atgattatct tcaccttacg ccgcattttg ttattgattg ttaccttgtt
    37381 cctgctgacc ttcgttggct tcagtttgag ctatttcact ccacacgcgc cgttacaagg
    37441 cgcgtcactg tggaatgcct gggtgttctg gtttaatggc ctgatccact gggattttgg
    37501 tgtttccagc attaacggtc aaccaattgc cgaacagtta aaagaggtct tcccggcgac
    37561 gatggaactg tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtggggat
    37621 gattgccggg attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttact
    37681 gggcttttca atcccggtgt tctggctggc gcttctgttg acgctgtttt gttcactcac
    37741 gctaggttgg ttgcccgttt cagggcgttt cgatctgctc tacgaagtga aaccgattac
    37801 cggttttgcg ttgattgatg cctggctttc ggactcacca tggcgggatg aaatgatcat
    37861 gagcgcaatc cgccatatga tattgcccgt gatcaccctg tcggttgcgc caacaactga
    37921 agtgatccgc ctgatgcgta tcagcactat cgaagtgtat gaccagaact atgtaaaagc
    37981 ggcggcgacc cgcggtttgt cgcgctttac tattttgcgt cgccacgttc tgcataacgc
    38041 gttaccgccg gttattcctc gcctgggctt acagttttcc accatgctga cgctggcgat
    38101 gatcaccgaa atggttttta gctggccggg tttagggcgc tggttaatta acgccatccg
    38161 ccagcaggat tacgcagcca tttccgccgg agtgatggtg tgtggctcac tggtcattat
    38221 tgttaacgtg atttctgata ttttgggtgc catggctaac cctctgaaac ataaggaatg
    38281 gtatgcctta cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct
    38341 ggcgcaaatt ttatagtgac gcctctgcga tggtcggcct gtacggctgc gcggggctgg
    38401 ctgtactgtg tatttttggc ggctggtttg cgccttacgg catcgaccag caatttctcg
    38461 gttatcaatt attgccgccg tcatggtcgc gctatggcga agtttctttc ttcctgggga
    38521 ctgacgacct cggacgcgat gttttaagcc gcttactgag cggagctgcg ccaaccgtgg
    38581 gtggcgcatt tgtggtgacg cttgccgcga cgatctgtgg cctggtactg ggcaccttcg
    38641 ccggggcgac acacggcctc cgctcagcgg tgcttaacca tattctggat accttactgg
    38701 cgatcccttc gctgctgttg gcaattatcg ttgtagcgtt tgccggaccg agtttgtccc
    38761 acgctatgtt tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg atttacagca
    38821 tggtgcatga cgaactggaa aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa
    38881 cgctgaatat tctctggttt gctgtgatgc caaacatcac cgccgggctg gtcaccgaga
    38941 tcacccgcgc actgtcgatg gcaattctcg atatcgccgc gctgggcttt ctcgatctcg
    39001 gcgcacaact cccctcgcct gaatggggag cgatgctcgg tgatgcgctg gaactgattt
    39061 atgtcgcccc gtggactgtc atgctgccag gtgcggcaat tatgattagc gtgttgttgg
    39121 ttaacctcct tggcgatggc gttcgtcgtg cgattattgc gggggtggaa taatgccatt
    39181 actcgatatt cgtaacctga ccattgaatt taaaaccggt gatgagtggg ttaaagccgt
    39241 cgaccgggta agtatgacct taaccgaagg tgaaatccgc ggtcttgttg gtgaatccgg
    39301 ttccggcaag agtttgattg cgaaagcgat ttgtggggtg aataaagata actggcgtgt
    39361 tactgctgac cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg cacgcgaacg
    39421 gcgcaaactg gttggccata acgtgtcgat gattttccag gaaccgcagt cgtgtcttga
    39481 cccttcagaa cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga cctataaagg
    39541 ccgttggtgg cagcgttttg gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt
    39601 ggggattaaa gatcacaaag atgcgatgcg cagttttccc tatgagttga ccgaaggtga
    39661 atgtcagaaa gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga
    39721 cgaaccgacc aactcaatgg agccaacaac ccaggcgcaa atctttcgcc tgctgacgcg
    39781 tctcaaccaa aacagcaata ccactatttt gcttatcagc catgacttac aaatgcttag
    39841 ccaatgggcg gataaaatta acgtgcttta ctgcggtcaa acagtggaaa ccgcgccgag
    39901 taaggagttg gtgacgatgc cacatcatcc ttatacccag gcgctgatcc gcgcgatacc
    39961 agacttcggc agcgcgatgc cgcataaaag tcgcctcaat acgctgcccg gcgctatccc
    40021 actgctggaa cagttaccga ttggttgtcg tctggggcca cgttgcccgt atgcacaacg
    40081 agaatgcatt gtgacgccac gtttgacggg ggcgaaaaat catctctatg cctgtcattt
    40141 cccgctgaac atggagaaag agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa
    40201 gtaaaacgtt ccgctaccgg accggctggt ttcgtcgtca gaccgtagaa gcggtaaaac
    40261 ccttgagctt tacgctacgt gaaggccaga cactggcgat tattggcgag aatggttcgg
    40321 gtaaatccac gctggcaaaa atgctggcgg gaatgataga gcctaccagc ggcgagttat
    40381 tgattgacga tcatccactg cattttggcg attattcctt ccgcagtcag cgtattcgca
    40441 tgatttttca ggatccttcg acctcgttga atccccgtca acgtatctcg caaattctgg
    40501 attttccact gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa cagattattg
    40561 aaacgatgcg tatggttggg ctgctaccgg atcacgtcag ttactatccg catatgttgg
    40621 cacccggaca aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc ccaaaagtca
    40681 ttattgccga tgaagccctc gcttcactgg atatgtcgat gcgttcgcag ttgattaatc
    40741 tgatgctgga gttacaggaa aaacagggca tttcgtatat ttatgttacc cagcatatcg
    40801 gaatgatgaa gcacattagc gaccaggtgc tggtgatgca tcagggcgag gttgtcgagc
    40861 gaggcagcac cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa cggctgattg
    40921 ccgggcactt tggcgaagca ttgacggcgg atgcgtggcg taaagatcgc taattttctg
    40981 aatcctcctt ccagattatc cacctcattc tgacaagatt taatctcttg tcagaatgag
    41041 gtgatggata aaataaaatc aaatgcgcgt gatttaagac gcaatctcac tttgcaggaa
    41101 cgaaagctct ggcgatatct tcgcagccga cgttttggtg atttcaaatt tcgccgtcaa
    41161 catccggtgg ggagctacat tctcgatttt gcttgctgct cggcgcgcgt agtcgttgag
    41221 ctggatggtg ggcagcatga tttggcagtt gcctatgata ccaggcgcac tagctggctt
    41281 gagtcgcagg gctggaccgt gctgcgtttc tggcataacg agattgattg taatgaggag
    41341 gcggtgctgg agattattct gcaggaactg aaccgccgat caccctcacc ctgaaagggc
    41401 gagggggccg accgagccga atagctgttg tggtgaaaac atggagacag tgctggagaa
    41461 tattcggcaa ggtctgaacc atcccagcca tcgccataaa agggttaggg gctgtatgag
    41521 cctgtttgtt gctggggtaa caatatttgc acaatatggt cccctcgccc ctctggggag
    41581 agggttaggg taaggggaaa agcgcccacc ctgccgcagc ctgctccggt cggacctggc
    41641 aactatagct actcacagcc aggttgatta taataaccgt ttatctgttc gtactgttta
    41701 ctaaaacgac gaatcgcctg attttcaggc acaacaagca tcaacaataa ggattaaagc
    41761 tatgggtttt ctttccggta agcgcattct ggtaaccggt gttgccagca aactatccat
    41821 cgcctacggt atcgctcagg cgatgcaccg cgaaggagct gaactggcat tcacctacca
    41881 gaacgacaaa ctgaaaggcc gcgtagaaga atttgccgct caattgggtt ctgacatcgt
    41941 tctgcagtgc gatgttgcag aagatgccag catcgacacc atgttcgctg aactggggaa
    42001 agtttggccg aaatttgacg gtttcgtaca ctctattggt tttgcacctg gcgatcagct
    42061 ggatggtgac tatgttaacg ccgttacccg tgaaggcttc aaaattgccc acgacatcag
    42121 ctcctacagc ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc
    42181 cctgctgacc ctttcctacc ttggcgctga gcgcgctatc ccgaactaca acgttatggg
    42241 tctggcaaaa gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga
    42301 aggtgtgcgt gttaacgcca tctctgctgg tccgatccgt actctggcgg cttccggtat
    42361 caaagacttc cgcaaaatgc tggctcattg cgaagccgtt accccgattc gccgtaccgt
    42421 tactattgaa gatgtgggta actctgcggc attcctgtgc tccgatctct ctgccggtat
    42481 ctccggtgaa gtagtccacg ttgacggcgg tttcagcatc gctgcaatga acgaactcga
    42541 actgaaataa tcgttctgtt agtaaagatg ggcggcgttc tgccgcccgt tatctctgtt
    42601 atacctttct gatatttgtt atcgccgatc cgtctttctc cccttcccgc cttgcgtcag
    42661 gataacgatt tcctttacga ccaaggagcg cccatggaac aacgccacat caccggcaaa
    42721 agccactggt atcatgaaac gcaatccagt actgcggagt atgacgttct gcctctggtc
    42781 ccggaagccg caaaggtcag cgatcccttt ctgctcgacg tgatccttga tgaagaaacg
    42841 ctggctccct tcctttcatg gctggtccct gcgcgcgttc ttgcagtgga attgttccct
    42901 gaccagctta ccgtgacccg ttcacagacc ttcaccgctt atgaacgctt gtctacggcc
    42961 ctgacggttg ctcaggtttg cggcgtccag cggttatgta actactattc ggcgcgactt
    43021 acgccgctcc ccgggcctga ttcctccagg gaaagcaatc ataggttggc acaaatcacg
    43081 caatatgccc gccaactggc tagctcgcct tctattatcg acaaccgatc gcgccagcat
    43141 ctgaatgacg tcggtcttac tgcctgggac tgtgtgatca ttaaccaaat cattggtttt
    43201 attggctttc aggcgcggac cattgcgaca tttcaggctt atctcgggca cccggtacgc
    43261 tggttacccg gtctggaaat acaaaactac gccgacgcgt cactgtttgc tgatgaatca
    43321 atacgctggc gaagcagcta tgaggtggaa aaactacctg aagagtacac aaaaagttca
    43381 acagcagaac tttgccaact ggccgaaaca ctctctctcc accctatttc actttccctt
    43441 ctcgaaaggt tgttaaacag tacacgggtt aatacacagc cggataataa gcttgcggcg
    43501 ttgttatgcg cgcggataaa tggcagtcct gcttgttttg ccgcctgtat ggattcatca
    43561 aatgaatata aaaaaatcag cccccttctg cgcaagggcg aaaatgaaat taaccaatgg
    43621 gctgaccgtc attctgttga gcacgctacc gttcaggcga tacaatggct gacccgagca
    43681 cccgatcgct ttagcgccgc ccagttcagc cctttactcg aacacgaaaa atcatcaacg
    43741 cagattatta atctgctggt atggagcggg ctgtgtggct ggataaatcg cttaaaaatc
    43801 gcgttgggtg agacatatta accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc
    43861 gctctaatgg ccaccaaaat agacaattat gtttcaggac aacccgctgc tagcgcagct
    43921 taaacagcaa ctgcattccc agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa
    43981 aggctttggc ttcctggaag tcgacgcgca aaaaagttat ttcattccgc cgccgcagat
    44041 gaaaaaagtc atgcatggcg accgaattat cgcggtgatc cacagtgaaa aagaacgtga
    44101 atccgcagag ccagaagaac tggttgaacc gttcctgact cgtttcgtgg gtaaagttca
    44161 gggcaaaaat gaccgtctgg ccatcgttcc tgatcatcca ctcttaaaag acgccattcc
    44221 ttgccgcgca gcccgtggcc tgaaccacga gtttaaagaa ggcgactggg cggttgccga
    44281 aatgcgccgt catccgctga aaggcgatcg ttctttctat gctgaactga cacaatacat
    44341 cacttttggt gacgaccact ttgtaccgtg gtgggttacc cttgcgcgcc ataatctgga
    44401 aaaagaagca ccagacggcg tcgctaccga aatgctcgat gaaggtctgg ttcgtgaaga
    44461 tctgaccgcg ctggattttg tcaccatcga cagtgccagc acagaagata tggatgacgc
    44521 ccttttcgct aaggcgttgc cggatgacaa acttcagctg attgtggcga ttgccgatcc
    44581 aaccgcgtgg attgctgaag gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac
    44641 caactatctg cctggcttca acatccctat gctgcctcgc gagctttctg acgatctctg
    44701 ctcactgcgc gccaatgaag tccgcccggt actggcatgc cgcatgacgc tctccgctga
    44761 tggcaccatt gaagataata tcgaattctt tgccgccacc atcgaatcca aagcaaagct
    44821 ggtgtatgac caggtttctg actggctgga aaataccggt gactggcagc ctgaaagtga
    44881 agcaattgcc gaacaagtcc gtttgctagc gcaaatttgc caacgccgcg gcgagtggcg
    44941 tcataaccac gcactggtgt ttaaagatcg cccggattac cgctttattc tcggtgaaaa
    45001 aggtgaagtg ctggatatcg tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga
    45061 agcgatgatt gccgctaaca tttgtgcggc ccgcgtactg cgcgataagc tcggttttgg
    45121 catctataac gtgcatatgg gctttgatcc ggcgaatgcc gacgcgctgg cagcgttgct
    45181 gaaaacgcac ggtctgcatg tcgatgccga agaagtgctc acgctggacg gtttctgcaa
    45241 actgcgtcgt gaactggacg cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt
    45301 ccagtcattt gctgaaatta gcactgaacc cggtcctcac tttggcctcg gtctggaagc
    45361 atacgccacc tggacttcgc cgatccgtaa atatggcgac atgatcaacc accgtctgct
    45421 gaaagcggtt atcaaaggcg aaactgcgac gcgcccacag gatgaaatca ctgtccaaat
    45481 ggccgagcgt cgccgtctca accggatggc agaacgtgat gttggtgact ggttatacgc
    45541 acgcttcctg aaagacaaag ccgggaccga cacccgtttc gcagcggaaa ttgtcgatat
    45601 cagccgtggc ggcatgcgtg ttcgtttggt tgataacggc gctatcgcct ttattccggc
    45661 acctttctta cacgctgtgc gcgatgaact ggtttgcagc caggaaaacg gcactgtaca
    45721 aattaaaggt gaaacggttt acaaagtgac tgacgttatt gacgtcacta ttgccgaagt
    45781 ccgcatggaa acccgcagca ttattgcgcg cccggtcgcg taatctcctt tcacggccca
    45841 ttcctcatgg atgggccgtt tatttccccg ctctaccttc gttatatcca ggcagatttt
    45901 ttaactgact ttcgtttgaa aactggcgtt tttccaaaac tggattacaa ataaatatat
    45961 aaaaaaaaca atgcgtttag ccgccgttaa tttcatgtaa gtcatgttgt tccttttatt
    46021 taaccgcgga ctccgcagtt aaccggagga tatgcatcat gaaaaccgtt agggagtcca
    46081 caacgttgta caactttctc ggatcgcaca atccatactg gcggttgacg gaaagcagcg
    46141 atgttttgcg cttttctacc accgaaacca cagaacctga tcgtactttg cagttatctg
    46201 ccgaacaggc tgctcgcatc agggaaatga cggtcatcac ctccagcctg atgatgagtc
    46261 tgaccgtcga tgagagcgat ctttctgtgc atctggtagg acgaaaaatc aataaacggg
    46321 aatgggctgg caacgcgtct gcatggcatg acacaccggc ggttgctcgt gatttatcac
    46381 acgggctttc ctttgctgag caggtagttt ctgaagcaca ttccgcaata gtcattctcg
    46441 acagccgggg gaatattcaa cgcttcaatc ggttatgtga agattacaca gggttgaaag
    46501 aacacgacgt cattgggcaa agcgtgttta aactgtttat gagccgtcgt gaagctgcgg
    46561 catccaggcg caataaccgt gtattttttc gaagcggcaa tgcatatgaa gtcgaactgt
    46621 ggataccaac atgtaaaggc cagcggctgt ttctgtttcg caataaattt gtccacagcg
    46681 gcagtggcaa aaacgagatt tttttaatct gttccggcac cgacattacc gaagagcgcc
    46741 gcgctcagga gcgactgcgt attctggcaa ataccgacag tatcaccgga ctgccgaatc
    46801 gtaacgcaat gcaggattta atcgatcacg ctattaatca tgcagataac aataaagttg
    46861 gggttgtgta tcttgatttg gataatttca aaaaggtcaa cgacgcctat gggcatttgt
    46921 ttggtgacca gttattacgc gacgtgtcat tggctatttt aagctgtctc gaacatgacc
    46981 aggtgttggc gcgtccaggt ggggatgagt ttctggtact ggcatccaac acctcacaaa
    47041 gcgcgctgga agcaatggca tcacgaattt tgacccgctt acggctcccc tttcgcattg
    47101 gtttaattga agtttatacc agctgttcag taggtattgc actctctccc gaacatggtt
    47161 cagacagcac ggctattatt cgtcacgccg acacagcaat gtacacagcg aaggaaggcg
    47221 gacgaggaca attttgcgtt tttaccccag aaatgaatca acgggtattt gaatatctct
    47281 ggctggatac caacttgcgt aaagcactgg aaaacgatca gttggttatt cactatcaac
    47341 cgaaaatcac ctggcgtggc gaagtgcgca gtctggaagc actagtacgt tggcagtcac
    47401 ctgaacgtgg gttgattcca ccgttggact tcatttccta cgccgaagag tcagggctaa
    47461 ttgtgccttt aggccgttgg gtgattctcg atgtcgtacg ccaggtggca aagtggcggg
    47521 ataaaggcat aaacctgcga gtggcggtaa atatttctgc acgtcagctc gccgatcaaa
    47581 ccattttcac cgccctgaaa caggttctcc aggaactcaa ttttgaatac tgccctatag
    47641 atgttgaact gacagagagt tgtctgattg agaatgatga actggcactg tctgttattc
    47701 aacaatttag ccaactaggt gcgcaagtgc atctggatga ttttggtacc ggctactctt
    47761 cactttcgca actggcgcgc tttccgatcg atgccatcaa acttgaccag gtttttgttc
    47821 gagatattca caaacaacct gtctcgcagt cactggtccg ggcgatcgtc gctgtggccc
    47881 aggcattgaa tcttcaggtg atcgccgaag gtgtagagag tgcaaaggaa gatgcttttt
    47941 taaccaagaa cgggatcaat gagcggcaag gatttttgtt tgccaaaccg atgcccgccg
    48001 tcgccttcga acgctggtat aaacgctatc tgaagcgcgc ataagtagat gaatatatat
    48061 acttagcgct atatcccgtc attatatatc gtattcctgg atgataatta gccgttgctg
    48121 ttaaacactt ctcttttagc ggcaattttt gcattattca ttttccactt ttgctataag
    48181 gatgtccttg atgtctgaat ttgatgccca gcgcgttgcc gaacgtattg atattgtgtt
    48241 agatatcctt gtggcaggcg attaccactc tgcgatccat aatctcgaga tacttaaagc
    48301 agaactcttg cgccaggttg ctgaatctac gccagacatc ccgaaggcac cgtgggagat
    48361 ttaacaatcg tgccttcagg ttttcctatt tgaggatgcc cggcgcgtca gtattttttt
    48421 atttagtatt ataacgttat aagaattaca gcgatgaatt cccgacaaca aactattcta
    48481 cagatggtca ttgaccaggg tcaggttagc gtaaccgatc tggcaaaagc cactggagtt
    48541 tctgaagtta ccattcgcca ggatctcaac accctcgaaa aactgagtta cctccgccgt
    48601 gcacatggct ttgcagtttc gcttgatagt gatgacgtcg aaacccgtat gatgagcaac
    48661 tatacgctga agcgtgaact cgccgagttt gccgcgtcac tggttcaacc gggcgaaacc
    48721 atctttatcg aaaatggtag cagcaatgcc ctgctcgccc ggactctggg ggagcagaag
    48781 aaaaatgtca ctatcatcac ggtcagcagc tacatcgcgc atttgctgaa agacgcgcct
    48841 tgtgaagtca ttttgctcgg tggcgtgtac cagaaaaaaa gcgaaagtat ggttggcccg
    48901 ttgacacgcc agtgcatcca gcaggtgcat ttcagcaaag catttattgg tattgatggc
    48961 tggcaacctg aaactggatt caccggtcgc gatatgatgc gtaccgatgt ggtcaatgcc
    49021 gtgctggaaa aagagtgcga agcgatagtc ctgactgaca gctcgaaatt tggtgctgta
    49081 cattcatact ccatcggtcc cgttgagcga ttcaatcgcg tgattaccga ttcgaaaata
    49141 cgcgccagcg atctgatgca tcttgagcac agtaaactca ccgttcacgt cgttgacatt
    49201 taaccatttc ccttctctac ggatgatttg cagtttggca aatcatccgc tctaagatga
    49261 ttcctggttg ataattaaga ctatttacct gttattaaca cgcttaagat ataaaattat
    49321 tatcagcgat ataacaggaa gtcattatca cctgcgtgat ataaccctgc gcgcgagcag
    49381 atttcacgga ataatt
//
LOCUS       DAJOWB010000020        48827 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJOWB010000020 DAJOWB010000000
VERSION     DAJOWB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.020"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            140..586
                     /locus_tag="OHO85_001326"
     CDS             140..586
                     /locus_tag="OHO85_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCQ2670727.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            597..2048
                     /locus_tag="OHO85_001327"
     CDS             597..2048
                     /locus_tag="OHO85_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCQ2670728.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTLVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2038..3108
                     /locus_tag="OHO85_001328"
     CDS             2038..3108
                     /locus_tag="OHO85_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCQ2670729.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAAFKNCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            3108..4856
                     /locus_tag="OHO85_001329"
     CDS             3108..4856
                     /locus_tag="OHO85_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCQ2670730.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNSVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNCITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(4906..5961)
                     /locus_tag="OHO85_001330"
     CDS             complement(4906..5961)
                     /locus_tag="OHO85_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HCQ2670731.1"
                     /translation="MKRNLLSSAIIVAIMSLGLTGCDDKKAETETLPPANSQPAAPAP
                     EAKPTEAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYADW
                     LKDFKQGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAV
                     ALGNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKR
                     QLAELKNIEEREGKTFHYYSLAVMISAKQINNLISQDKFDAEAAMKKVSELETLVAQA
                     KEADKGGMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFP
                     RALREYNEMVDDYNSLR"
     gene            complement(6114..6947)
                     /gene="fdhD"
                     /locus_tag="OHO85_001331"
     CDS             complement(6114..6947)
                     /gene="fdhD"
                     /locus_tag="OHO85_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCQ2670732.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDGALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7141..10191
                     /gene="fdnG"
                     /locus_tag="OHO85_001332"
     CDS             7141..10191
                     /gene="fdnG"
                     /locus_tag="OHO85_001332"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:7726..7728,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCQ2670733.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            10204..11106
                     /gene="fdoH"
                     /locus_tag="OHO85_001333"
     CDS             10204..11106
                     /gene="fdoH"
                     /locus_tag="OHO85_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCQ2670734.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNVGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            11103..11738
                     /gene="fdoI"
                     /locus_tag="OHO85_001334"
     CDS             11103..11738
                     /gene="fdoI"
                     /locus_tag="OHO85_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCQ2670735.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            11735..12664
                     /gene="fdhE"
                     /locus_tag="OHO85_001335"
     CDS             11735..12664
                     /gene="fdhE"
                     /locus_tag="OHO85_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCQ2670736.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKDPKIEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(12994..13236)
                     /locus_tag="OHO85_001336"
     CDS             complement(12994..13236)
                     /locus_tag="OHO85_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670737.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(13454..13672)
                     /locus_tag="OHO85_001337"
     CDS             complement(13454..13672)
                     /locus_tag="OHO85_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCQ2670738.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(14529..15470)
                     /gene="fabY"
                     /locus_tag="OHO85_001338"
                     /gene_synonym="yiiD"
     CDS             complement(14529..15470)
                     /gene="fabY"
                     /locus_tag="OHO85_001338"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCQ2670739.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(15515..15952)
                     /gene="dtd"
                     /locus_tag="OHO85_001339"
     CDS             complement(15515..15952)
                     /gene="dtd"
                     /locus_tag="OHO85_001339"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCQ2670740.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(15949..16821)
                     /locus_tag="OHO85_001340"
     CDS             complement(15949..16821)
                     /locus_tag="OHO85_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCQ2670741.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(16815..17414)
                     /gene="yihX"
                     /locus_tag="OHO85_001341"
     CDS             complement(16815..17414)
                     /gene="yihX"
                     /locus_tag="OHO85_001341"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCQ2670742.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(17513..18298)
                     /locus_tag="OHO85_001342"
     CDS             complement(17513..18298)
                     /locus_tag="OHO85_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCQ2670743.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(18332..19228)
                     /gene="yihV"
                     /locus_tag="OHO85_001343"
     CDS             complement(18332..19228)
                     /gene="yihV"
                     /locus_tag="OHO85_001343"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCQ2670744.1"
                     /translation="MIRVACVGITVMDRIYYVKGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            19396..20292
                     /gene="yihU"
                     /locus_tag="OHO85_001344"
     CDS             19396..20292
                     /gene="yihU"
                     /locus_tag="OHO85_001344"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCQ2670745.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     SGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            20316..21194
                     /gene="yihT"
                     /locus_tag="OHO85_001345"
     CDS             20316..21194
                     /gene="yihT"
                     /locus_tag="OHO85_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCQ2670746.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            21211..22452
                     /gene="yihS"
                     /locus_tag="OHO85_001346"
     CDS             21211..22452
                     /gene="yihS"
                     /locus_tag="OHO85_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCQ2670747.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            22590..23492
                     /locus_tag="OHO85_001347"
     CDS             22590..23492
                     /locus_tag="OHO85_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCQ2670748.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            23691..25727
                     /gene="yihQ"
                     /locus_tag="OHO85_001348"
     CDS             23691..25727
                     /gene="yihQ"
                     /locus_tag="OHO85_001348"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCQ2670749.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAAALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            25773..27158
                     /locus_tag="OHO85_001349"
     CDS             25773..27158
                     /locus_tag="OHO85_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2670750.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            27201..28604
                     /locus_tag="OHO85_001350"
     CDS             27201..28604
                     /locus_tag="OHO85_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2670751.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFGNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            28672..29364
                     /gene="ompL"
                     /locus_tag="OHO85_001351"
     CDS             28672..29364
                     /gene="ompL"
                     /locus_tag="OHO85_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCQ2670752.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(29455..30720)
                     /locus_tag="OHO85_001352"
     CDS             complement(29455..30720)
                     /locus_tag="OHO85_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2670753.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMISVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(30822..31802)
                     /locus_tag="OHO85_001353"
     CDS             complement(30822..31802)
                     /locus_tag="OHO85_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCQ2670754.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(31810..32520)
                     /locus_tag="OHO85_001354"
     CDS             complement(31810..32520)
                     /locus_tag="OHO85_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCQ2670755.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPKTLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(32737..34560)
                     /gene="typA"
                     /locus_tag="OHO85_001355"
     CDS             complement(32737..34560)
                     /gene="typA"
                     /locus_tag="OHO85_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCQ2670756.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            34933..36342
                     /gene="glnA"
                     /locus_tag="OHO85_001356"
     CDS             34933..36342
                     /gene="glnA"
                     /locus_tag="OHO85_001356"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCQ2670757.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            36661..37710
                     /gene="glnL"
                     /locus_tag="OHO85_001357"
     CDS             36661..37710
                     /gene="glnL"
                     /locus_tag="OHO85_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCQ2670758.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            37722..39131
                     /gene="glnG"
                     /locus_tag="OHO85_001358"
     CDS             37722..39131
                     /gene="glnG"
                     /locus_tag="OHO85_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCQ2670759.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            39243..39353
                     /locus_tag="OHO85_001359"
     CDS             39243..39353
                     /locus_tag="OHO85_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCQ2670760.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(39543..40916)
                     /gene="hemN"
                     /locus_tag="OHO85_001360"
     CDS             complement(39543..40916)
                     /gene="hemN"
                     /locus_tag="OHO85_001360"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCQ2670761.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(41105..41614)
                     /gene="yihI"
                     /locus_tag="OHO85_001361"
     CDS             complement(41105..41614)
                     /gene="yihI"
                     /locus_tag="OHO85_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCQ2670762.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVIEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            42196..42828
                     /locus_tag="OHO85_001362"
     CDS             42196..42828
                     /locus_tag="OHO85_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCQ2670763.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(43210..45996)
                     /gene="polA"
                     /locus_tag="OHO85_001363"
     CDS             complement(43210..45996)
                     /gene="polA"
                     /locus_tag="OHO85_001363"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCQ2670764.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPNDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            46360..47292
                     /locus_tag="OHO85_001364"
     CDS             46360..47292
                     /locus_tag="OHO85_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCQ2670765.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEDLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(47333..48763)
                     /locus_tag="OHO85_001365"
     CDS             complement(47333..48763)
                     /locus_tag="OHO85_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCQ2670766.1"
                     /translation="MIHKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVATETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRD
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT        11920 a        12499 c        12058 g        12350 t
ORIGIN      
        1 taaccattat gtgcattact ccgcaaatct gacctttcac tctgtctcaa attgtcattg
       61 tttaccgaca tatcgcctct catcacttaa tcgctccacc attacaattg attaaaatat
      121 attttatgga gtccgattaa tggcagctct tactgcaagc tgtattgacc tgaatattca
      181 gggcaatggc gcttattccg ttctgaagca gttggcgaca atagcgttac aaaacggttt
      241 tatcaccgac tcacaccagt tcctgcaaac cctgctgctg cgcgaaaaaa tgcactctac
      301 tggatttggt tccggtgtcg ccgtgccgca cggtaaaagc gcctgcgtta aacaaccgtt
      361 cgtattattc gcccgcaaag cgcaggctat tgactggaaa gccagcgatg gcgaagacgt
      421 caattgctgg atctgcctcg gcgtgccgca aagcggcgaa gaggatcagg tcaaaatcat
      481 cggcacactg tgtcgcaaaa ttatccacca ggattttatt catcaactgc aacagggcga
      541 cgccgaccag gtgcttgcct tgttaaatca aaccctcagc tcataaggaa gtggcgatgg
      601 agtcatcctt acgtattgtc gcgatcacca actgccccgc cgggatcgct cacacctaca
      661 tggtggcgga agccctggaa cagaaagccc gttctctcgg ccataccata aaagtggaaa
      721 ctcaagggtc cagtggcgtt gaaaaccgct tatccagcga agagattgcc gctgccgatt
      781 acgtcattct cgctaccggg cgtggcctga gcggtgatga tcgcgcgcgg tttgccggga
      841 agaaagttta tgagattgcc atctcccagg cgttgaaaaa tatcgaccag attttcagcg
      901 aattaccgac aaactcgcag ctttttgccg cagatagcgg cgtgaagctt ggcaaacagg
      961 aagtgcaaag cggcagcgtg atgagccacc tgatggcggg cgtctctgcg gcgctgccgt
     1021 ttgtcatcgg cggtggtatc ctggtggcgc ttgccaacat gctggtgcag ttcggtttgc
     1081 cgtataccga tatgtcgaaa ggcgcgccgt cgtttacctt ggtagttgaa tccatcggtt
     1141 accttggctt cacctttatg atccccatca tgggcgctta tattgcctcg tcgattgccg
     1201 ataaaccggc tttcgctccg gcgtttctgg tttgctatct ggcgaacgac aaagcgctgc
     1261 tcggcaccca gtcgggtgcg ggattcctcg gcgcggtggt gctggggctg gcgattggct
     1321 atttcgtctt ctggttccgc aaagtgcgcc ttggcaaagc attacaaccg ctgctcggtt
     1381 caatgctgat cccgttcgtt actctgctgg ttttcggcgt gctcacttac tacgttatcg
     1441 ggccggtgat gtccgacctc atgggcgggc tgctccactt cctgaatacc attcctccat
     1501 caatgaagtt tgcggcagcg tttctggtcg gtgcgatgct ggcgttcgat atgggcggcc
     1561 ccatcaataa aaccgcctgg ttcttctgct tctcactgct ggaaaaacac atttatgact
     1621 ggtacgccat cgtcggcgtt gtcgcgttga tgccgcccgt tgcggcgggt cttgcgacgt
     1681 tcattgcgcc caaactgttt acccgacagg aaaaagaggc tgccagtagt gccattgtgg
     1741 tcggtgccac agtcgcaacc gaaccggcta taccttacgc tctggcagct ccgttgccga
     1801 tgattaccgc caatacgctg gcgggtggca ttaccggcgt gctggtgatc gctttcggaa
     1861 tcaaacgtct ggcacctggt ttgggtatct ttgacccgct gatcggcctg atgtcgccgg
     1921 tgggttcgtt ttatctggtg ctggcgattg gcctcgcgct gaacatttca ttcattatcg
     1981 tcctgaaagg attgtggtta cgccgcaaag cgaaagccgc gcaacaggag cttgtccatg
     2041 aacattgagt tactgcaaca gttgtgcgaa gccagcgccg tcagcggcga tgaacaggaa
     2101 gttcgcgaca ttctgataaa cacgctggaa ccttgcgtta atgagatcac ctttgatggt
     2161 ctgggcagct ttgttgcccg taaggggaat aaaggtccaa aagttgccgt tgtcgggcat
     2221 atggatgaag tcggctttat ggtcacccac atcgacgaga gcggttttct gcgtttcacc
     2281 accattggtg gctggtggaa tcagtcgatg ctcaaccacc gggtaaccat acgcacacac
     2341 aagggagtga aaatccctgg tgtgattggt tccgtcgcgc ctcatgcgtt aacggaaaag
     2401 caaaggcaac agccgctgtc atttgatgag atgttcattg atattggcgc gaacagtcgc
     2461 gatgaggtgg aaaaacgcgg tgtggcgatg ggcgatttta ttagcccgga agccaatttt
     2521 gcctgctggg gcgaagataa agtggtcggc aaggcgctgg ataaccgcat cggctgcgca
     2581 atgatggctg aactattgca gacggtgaat aatcccgaaa ttacgctgta tggcgtcggc
     2641 agcgtggaag aagaagttgg gctacgcggg gcacaaacct cggcggaaca cattaaaccg
     2701 gatgtggtga ttgtgctgga taccgctgtc gcaggtgatg ttccgggcat tgataacatt
     2761 aaatacccgc tgaaactggg ccaggggccg gggctgatgc tgtttgacaa gcgctacttc
     2821 cccaaccaga aactggtagc agcgtttaaa aactgtgcca cgcagaatga tttaccgctg
     2881 caattttcca ccatgaaaac cggagcgacg gatggcgggc gctacaacgt gatgggcggc
     2941 gggcgtccgg ttgtcgcgct gtgtctgcca actcgttatc tgcacgccaa cagcggtatg
     3001 atttcaaaag ccgattacga tgctctgctc acgctgatac ggggttttct gacgacctta
     3061 actgcggaga aagtcaacgc gtttagccag ttccgtcagg tggattaatg ctaaacgaac
     3121 gccagttaaa gattgtcgat ctgctggagc aacagccgcg cacgcctggc gagctggcgc
     3181 aacagactgg cgtttcaggc aggaccatcc tgcgtgatat tgactatctc aacttcaccc
     3241 ttaacggcaa agcccgcatt ttcgccagtg gcagtgcggg ctatcagctg gaaatcttcg
     3301 agcgccgcag cttttttcag ttgctgcaaa agcacgataa cgacgatcgg ctgctggcgc
     3361 tgttattact gaatactttc actccccgtg cgcaactcgc ctcggcgctt aatttgccag
     3421 aaacgtgggt agcagagcgt ctgccccggt taaaacagcg ttatgaacgc acttgttgcc
     3481 tggccagccg ccctggtttg ggccatttca ttgatgagac agaagagaaa cgcgttatct
     3541 tgctggcgaa cttgctgcgc aaagatccgt ttttaattcc gctggcgggc ataacacgag
     3601 acaaccttca gcatttatcc acggcctgcg acaaccaaca ccgctggccg ctcatgcagg
     3661 gtgattatct ctccagcctg attctggcga tttacgccct gcgtaatcaa ctgaccgatg
     3721 agtggccgca atatcccggt gacgagataa aacaaatcgt tgaacatagc ggtctgtttc
     3781 tcggtgataa ttctgtaaga accctgacgg gtttgataga gaaacagcat cagcaagcgc
     3841 aggtaatttc agccgatcat gtgctggggt tgctgcaaag ggtgccaggc atcgcgtcat
     3901 tgaatattat tgatacgcag ctggttgaga atattaccgg gcatttatta cgttgccttg
     3961 ccgcaccggt gtggattgct gagcaccgcc aaagcagcat gaataacctg aaagcagcct
     4021 ggccagcggc ctttgatatg agtctgcact ttattacgct actgcgtgaa cagctcgata
     4081 ttcccctttt cgacagcgat ctgatcggtt tgtattttgc ctgtgcgctg gaacggcatc
     4141 aaaacgaacg ccagccgatc attttgctct cggaccagaa cgcgattgcc actattaatc
     4201 agctcgccat tgagcgcgat gttttacatt gtcgggtgat tattgcccgt agcttaagcg
     4261 aacttgttgc cattcgcgaa gagattgagc cgttattgat cattaacaac agccattatt
     4321 tactggatga cgcggtaaat aattgcatca ccgtaaaaaa tatcattacg gctgccggta
     4381 tcgaacaaat aaaacatttt ctggcgacgg catttattcg ccagcaaccg gagcgttttt
     4441 tctctgcccc cggaagtttt cattattcga atgtacgcgg tgaaagctgg caacatatta
     4501 cccggcaaat ttgtgcgcaa ttagtggcac aacaccatat taccgccgat gaagcgcaac
     4561 ggatcatcgc ccgcgaaggc gaaggtgaaa acctgattgt taatcgcctc gccatcccac
     4621 attgctggag cgaacaggag cgacgttttc gtggattttt tattaccctc gcccaaccag
     4681 ttgaggtgaa taacgaagtc attaaccatg tcttgatcgc ctgcgccgcc gccgatgcgc
     4741 gtcacgagtt gaaaatattc agctatctgg caagcgtatt gtgtcagcat ccggcagagg
     4801 ttattgccgg gttaacagga tatgaggcat ttatggagtt acttcacaag gggtgaactg
     4861 tgtcggatgc gccgcatccg acactgcatt tatcatcaaa aacgtttaac gcaggctatt
     4921 atagtcatca accatttcgt tgtactcgcg taatgctctc gggaaagagt cttcgaccat
     4981 ccagcttgag tttgcgtcct gaagttgctc tttatcccag tcagagtacg ggactttatc
     5041 tctgatgcgg cgaacgtatt ttttagcctc gagttgatac tggcctgccg aattaataaa
     5101 cgagaaattc atgccgcctt tatccgcttc tttggcctgc gccaccagcg tttccagttc
     5161 agacactttc ttcattgccg cttctgcatc aaacttatct tgcgatatca ggttattaat
     5221 ttgtttcgct gaaatcatga ctgccagaga gtagtagtgg aatgttttcc cttctctttc
     5281 ttcaatattt ttcagttccg caagctgacg cttatcatta atttcctgaa tcgccgcatg
     5341 ataagattcg gctacaggtt caaaggcttc cagatttttt aagaatgtct ggtgcagcgt
     5401 tttccctttc gcaaacgcat cgtctttata attttcctgg gtgtaatatt tatccatttc
     5461 gttaatggta ttacccaacg ccacggcagc atcgatataa ctcaccgcca cgccatcaat
     5521 tggttgcagc gcaggcgtta acgccacagc actttttacg cctttttcac actcagcgag
     5581 gttggattca ctaatgccgt agatgccata aacagtacgc tcttcaccgg taggcccctg
     5641 tttaaaatct ttcagccagt cagcataacg cgccagactg cgctgtaccg ggatctgtaa
     5701 cttgttgtag cacttgatat agacatccat tttttcgtcg aaaaccgctt gttcatcgac
     5761 caccggttgc gcaggtgttt caggtttagc ttctgctttt gcaacgggag cttcagtagg
     5821 tttcgcttca ggagctggtg cggcaggttg actattggca ggcgggagcg tttctgtttc
     5881 ggctttttta tcatcacaac ccgtcagacc gagggacatg atggcgacta taatagcgga
     5941 agataataaa ttccttttca tttaattgcc ttataaatat atagataaga gagtgaacat
     6001 aataaatgaa aaaagaaaac gccactacac gcattttgtt tataaataaa ataataaaat
     6061 tcacgaaatg aataagtcct gcaatttaat atatttcgca ggacttattt taattaattg
     6121 ctcaaacgct gcggatgggt ataaaccgtt gcccgacccg gtttacaaaa acctaccagt
     6181 gtcagattac agcgctcagc cacttctaca gcaagcgtgg tcgcggcaga caccgcaaac
     6241 aaaatctcta cgccgcacat cgccgacttt tgcaccattt cataactggc acgactggaa
     6301 accagtaccg caccttgctg ccagctttcc ccttcttgtg accggcggcc taacagtttg
     6361 tccagcgcca catggcgacc cacgtcttca tgcccgccga ccagttcgcc agatggcaac
     6421 atccaggcag cggcgtgagt acaaccagtc agttgcccca ctggctgaaa atcgttgaga
     6481 tgacgtaatg caccatccag tttgttgaga tcaaacgtct gggtgaacgg tagcggctgc
     6541 accggttttc cgatgtcatt aagttgctcc acgccgcata cgccacagcc cgtacgtccc
     6601 gccagcgccc ggcggcgctc cttcaacccc ataaagcggc ggctggaaag ctcaatttgt
     6661 acttcaagac cattacagga aggaacgaca tccatgccga agatatcgcg cggactttcg
     6721 ataatcccct cggaaagcga aaaaccgagc gcaaagtact caaggtcttt gggcgacgcc
     6781 atcatcacca catgcgaaat gccgttgtag accagcgcaa cgggaacttc ttccgcgacc
     6841 tcatctaacc gtgggtgttg taaatcatca cgccgccata actcaatttg acgaacacct
     6901 gtgatatttg tcacattttc gatttctttt cgctgtgttt ttttcacttt ctcttaacca
     6961 gataagaaca cacacaccaa cattatggta ttctgttaca aacccttcct ggatggaggg
     7021 aaattgagcc aattctggac ctttgcggcc ccttccgcaa agaaaaataa ctcccactcc
     7081 ctgcacacgc agcaagcgaa tgtaaatggg acgtgacaat gtcgaaacaa ggagcaatcc
     7141 atgcaggtca gcagaaggca gttctttaag atctgcgctg gcggtatggc aggcaccacg
     7201 gcagcggcac tgggttttgc acccagcgta gcactcgcgg aaacccggca gtataaactg
     7261 ctgcgcaccc gcgaaacccg taatacctgc acctattgtt ccgtaggctg tgggctgttg
     7321 atgtacagcc tcggtgacgg agcaaaaaac gccaaagcat ctatcttcca tatcgaaggc
     7381 gatccggatc acccggtcaa ccgcggtgca ctttgtccga aaggcgctgg cctggtggat
     7441 ttcatccact ccgaaagccg tctgaagttt ccggaatacc gtgcgccagg ttctgataaa
     7501 tggcagcaaa tcagttggga agaggcgttt gatcgcatcg ccaaactgat gaaagaagac
     7561 cgcgatgcta actacattgc gcaaaacgcc gaaggcgtga ctgttaaccg ctggctctcc
     7621 accgggatgc tgtgtgcttc cgcgtcgagt aacgaaaccg gctatttaac gcaaaaattc
     7681 tcccgcgcgc tgggtatgct cgcggtcgac aaccaggcgc gtgtctgaca cggaccaacg
     7741 gtagcaagtc ttgctccaac atttggtcgc ggtgcgatga ccaaccactg ggtcgacatc
     7801 aagaacgcca acctcgtcgt ggtgatgggc ggtaacgccg ctgaagctca cccggtcggg
     7861 ttccgctggg cgatggaagc caaaattcac aacggcgcga agctgattgt gatcgatcct
     7921 cgctttacgc gtacggcggc ggtagctgac tactatgccc ctattcgttc cggtactgac
     7981 attgctttcc tgtcaggcgt attgctgtac ctgctgaaca atgaaaaatt caaccgcgaa
     8041 tacaccgaag cctataccaa cgccagcctg atcgtgcgtg aggattacgg ctttgaagat
     8101 ggcctgttca ccggctacga cgcggaaaaa cgcaagtacg ataaatcctc ctggacttat
     8161 gaactggacg aaaacggctt cgccaaacgc gataccacgc tgcaacatcc gcgctgcgtg
     8221 tggaacttgc tgaaacagca cgtttcccgc tacacgccag atgtggttga aaacatctgt
     8281 ggtacgccaa aagacgcgtt cctgaaagtc tgcgaataca tcgcagaaac cagtgctcac
     8341 gataaaactg cctcgttcct gtatgccctc ggctggacgc aacactccgt tggtgcgcaa
     8401 aacattcgta cgatggcgat gatccagctg ctgctcggca acatggggat ggcaggcggc
     8461 ggcgttaacg ccctgcgcgg tcactccaat attcaggggc tgacggacct ggggctgctg
     8521 tcgcagagcc tgccaggtta catgacgctg ccaagcgaga agcagaccga tctgcaaacc
     8581 taccttaccg ccaacacgcc aaaaccgctg ctggaaggcc aggtaaacta ctggggcaac
     8641 tacccgaaat tcttcgtctc catgatgaag gccttctttg gtgataaagc gacggcggaa
     8701 aatagctggg gctttgactg gttgccgaag tgggataaag gctacgacgt cctgcaatac
     8761 ttcgagatga tgaaagaggg caaggtcaat ggctatatct gccagggctt taaccctgtt
     8821 gcctcgttcc cgaacaaaaa caaagtgatc ggttgtctgt cgaaactgaa gttcctcgtc
     8881 accatcgacc cgctgaacac tgaaacctct aacttctggc agaaccacgg tgagctgaac
     8941 gaagttgact cgtcgaagat ccagaccgaa gtgttccgtc tgccatcgac ctgcttcgca
     9001 gaagagaacg gttcaatcgt taactcaggt cgctggttgc agtggcactg gaaaggtgcg
     9061 gacgccccgg ggattgcgct gactgacggt gagatcctct ccggtatctt cctgcgcttg
     9121 cgcaagatgt atgccgaaca gggtggcgcg aacccggatc aggtgctgaa catgacctgg
     9181 aactacgcta ttccgcatga gccatcttca gaagaagtgg cgatggagag caacggtaag
     9241 gcgctggccg atattaccga tccggcaacc ggtgcggtta tcgtcaagaa aggccaacaa
     9301 cttagctcgt ttgcccaact gcgcgatgac ggtacaactt cctgtggttg ctggattttc
     9361 gccggtagct ggacgccgga aggcaaccag atggcacgcc gtgataacgc cgatccgtct
     9421 ggcctcggta acacgctggg ctgggcatgg gcatggccgc ttaaccgccg cattctgtat
     9481 aaccgcgcct ccgcagatcc gcagggtaac ccgtgggatc cgaagcgtca gttgctgaaa
     9541 tgggacggca ctaagtggac cggctgggat attccggact acagcgcagc gcctccgggc
     9601 agcggcgtcg ggccgtttat catgcagcag gaaggcatgg gacgtctgtt tgccctcgat
     9661 aagatggcgg aaggtccgtt cccggaacac tacgagccgt ttgaaacgcc gctgggaact
     9721 aacccgctgc atccaaacgt tatctcgaac ccggctgcgc gaatctttaa agacgacgcc
     9781 gaagcattgg gtaaagccga taagttcccg tatgtcggaa ccacctatcg tctgaccgag
     9841 cacttccact actggaccaa acacgcgctg ttgaacgcga ttttgcagcc agagcagttt
     9901 gtggaaatcg gtgagtcgct ggcgaataaa cttggcattg cccagggcga taccgtgaaa
     9961 gtctcctcca accgtggcta tatcaaagcc aaagcggtgg tgaccaaacg tattcgcacg
    10021 ctgaaagcga acggcaaaga tatcgatacc atcggtattc cgattcactg gggctatgaa
    10081 ggcgtagcta aaaaaggctt tattgccaat acgttaacgc cattcgttgg tgatgcgaac
    10141 acgcagacgc cggagtttaa gtccttcctt gtgaatgtgg aaaaggtgta acggagacga
    10201 catatggctt atcaatctca agatatcatt cgtcgttccg cgactaacgg tctgaccccc
    10261 gcgcctcaag cgcgggactt ccaggaagaa gtggcgaaac tcatcgacgt caccacctgt
    10321 atcggctgta aagcctgtca ggtggcgtgt tcagagtgga acgacattcg cgataccgtc
    10381 ggcaataacg ttggggtgta cgacaacccc aatgatttaa gcgccaaatc gtggacggta
    10441 atgcgcttct cggaagtgga gcagaacgac aaactggaat ggctgatccg caaagacggc
    10501 tgtatgcact gttccgatcc aggctgcctg aaagcgtgcc cggcggaagg tgcaattatt
    10561 cagtatgcca acggtatcgt cgacttccag tccgagcagt gcattggctg cggttattgc
    10621 attgcgggct gtccgttcga cattccgcgc ctcaacccgg aagacaaccg cgtctacaaa
    10681 tgtacgctgt gcgttgaccg cgtggtggtt gggcaagaac cagcctgcgt gaagacctgc
    10741 ccaacgggcg cgattcactt cggtacgaaa gagtcgatga aaacgctggc gagcgagcgc
    10801 gttgctgagc tgaaaacccg cggttacgac aatgcgggtc tgtacgatcc ggcaggcgtc
    10861 ggtggtacac acgtcatgta cgtgctgcac catgctgaca agccaaatct gtatcatggc
    10921 ttgccggaga acccggaaat cagcgaaacc gtgaaattct ggaaaggcat ctggaaaccg
    10981 ctcgcggctg ttggctttgc ggctaccttt gcggccagta tcttccacta cgtgggtgtc
    11041 ggtccgaacc gtgcggatga ggaagagaat aatctgcacg aagagaaaga cgaggagcgc
    11101 aaatgaaacg acgtgacacc atcgtgcgct acaccgcgcc ggaacgtatc aaccactgga
    11161 tcaccgcctt ctgcttcatc ctggcggcgg tgagcgggct gggctttttg ttcccgtcct
    11221 tcaactggtt gatgcaaatc atgggcacac cgcagctggc gcgaattctg cacccgtttg
    11281 tcggcgtggt tatgtttgcc tcgttcatca tcatgttttt ccgttactgg catcacaacc
    11341 taatcaatcg ggatgatatc ttttgggcga agaatattcg taagatcgtc gtcaacgagg
    11401 aagtaggtga caccgggcgt tataacttcg gtcagaaatg cgttttctgg gcggcgatta
    11461 ttttcctggt tctgctgctg gtgagcggcg tgattatctg gcgtccttat tttgcgcctg
    11521 ctttctcaat cccggtgatc cgattcgcgt taatgctgca ttcatttgcc gcagtggcgt
    11581 taattgtggt tatcatggtg catatctacg ccgccctttg ggtgaaaggc acgattaccg
    11641 ccatggtgga aggatgggtt accagcgcct gggcgaagaa acatcacccg cgctggtacc
    11701 gtgaggtccg caagacaacg gaaaagaaag ctgaatgagt attcgcataa tcccgcaaga
    11761 tgagctgggt tcaagcgaga aacgtacggc ggatatgatt ccgccgttat tgttccctcg
    11821 gctcaagaat ttatacaacc gccgcgccga gcgtctgcgc gagctggcag aaaataatcc
    11881 tctgggtgat tacctgcgct ttgctgcgct tatcgcccac gcccaggaag tggtgctgta
    11941 cgaccatccg ctggagatgg atctgactgc gcgcattaaa gaagccagcg cacaaggcaa
    12001 gcccccgctg gatattcacg ttctgccgcg tgataagcac tggcaaaagc tgctgatggc
    12061 gctgattgct gagctgaaac ctgaaatgag cggcccggcg ctggcagtga ttgagaatct
    12121 ggagaaggca tcgactcagg agctggaaga tatggccagc gcgctgtttg cctctgattt
    12181 ctcgtccgtc agcagcgata aagcgccgtt tatctgggct gcactgtcgc tctactgggc
    12241 gcagatggcc aatctgatcc ccggcaaagc ccgcgctgaa tacggcgagc aacgtcaata
    12301 ttgcccggtt tgtggttcta tgccggtgtc cagcatggtg caaattggca ccactcaggg
    12361 gctacgttac ctgcactgca acctgtgtga aactgaatgg cacgtagtgc gcgtaaaatg
    12421 cagcaactgc gagcagagcg gcaaactgca ttactggtcg ctggatgacg aacaggccgc
    12481 gattaaagcc gaaagctgcg atgactgcgg cacttacctg aaaattctct atcaggaaaa
    12541 agatccaaaa atcgaagccg tagcagatga cctcgcctct ctggtactgg acgcacgaat
    12601 ggagcaagaa ggctatgccc gcagttccat caacccgttc ctgtttccgg gtgaaggaga
    12661 gtaatctctg acgttttccg ggtggcgcag gtcgcccgga ttacagctca tagggtgtga
    12721 cgacaccatc tctcgtattc cgcgttacca cctcaagcta tgccgccagt gccggtttgg
    12781 cctcaattta cccaccacca catcaataag gttatgggca tgagactgat gacaagcgta
    12841 agattgcctg atgcgctgcg cttatcaggc ctaccaataa actgcaatac attgaattta
    12901 tacgactttg taggccggat aaggcgttca cgccgcatcc ggcatgaaca acgcgcacgt
    12961 tgtcagcaat ctgattgaca tcattttttc ccattaccat tcctcgcgca gcttacgctg
    13021 atattcgaca ccatcttcct cacacccttg ccagatgcca ggaacactgg ttcttgctgt
    13081 ttgcgattga tttatcaggt attgatctac cgcttccctt aataggtccg cacgaggaag
    13141 attacgctgc acctcgagat catcaagttg cttaatcacc tcattcgata aatcgagtaa
    13201 aattctgccc atatctatac ctgccagaga gttcataaat atctcgccaa tatcattttt
    13261 gaatctatgg agagaaaagt acccttgccg aatctttaaa gaaagctcat ttacgcatca
    13321 ttttttattt ttgcgaggcg ttttccagat atgatgtaac agttgcagtt attataagtt
    13381 caagtaaaac agccttgtat taataccaga ataagggcat ctgtttttta tattcaagaa
    13441 tgaaaaattt ttgtcattcc ttatgctcct tacactgatc atcaacacca actacctccc
    13501 ccttttgcct gcctcccttt gctttacgca ccgggaattg ctgacgttcc agatattgct
    13561 ctaccgcttc gcgtaaaatt gcagatcgtg aaaccttacg ccagccccga agcttgttca
    13621 gccgtttaat tgcctcttct gataaatgaa gaaaaaccgt attcatcgcc attaccacca
    13681 ccttacctat attactcatt aatgtatgtg cgaaaattac gccggaaaat catttaaaaa
    13741 aagcggattt ttgcatgttg ttttgttttt gcgaggcgtt ttccagaaat aataagagtg
    13801 ttgcagttgt ttcatttatt actcaaaaat ggtttagtta tcgcccagga tgaattatgg
    13861 gtgctatgaa tatattatat tcagagatat ttatttgcat aatctgaatg gtgtagccga
    13921 aaaacgtcag catcctaaca gcaccaggat ttaaggtgaa attaatcttt catcacagac
    13981 cggtcagtac tgccaccaac cggtctttac ttgctcatcg ttataatttt gttggtaaca
    14041 gttcgggaaa gttgtttttc taaattaaca acagaataac tttcttgttt aatgagatta
    14101 atattggcaa ccattatagc ctccttccca cggtttaaga gcaaacatac aacataatta
    14161 ttcataacat acagagcagt aaatgaatga tactgacaat gatgctagtc cattatcttt
    14221 gcataatatt tgttagctat agattttaaa aatatttatg agaaaagtat ggcaccagag
    14281 ggggttttga ctatcaaaaa tcgctcaaac ctgaactaag atgatgagtt aagcattatc
    14341 tgcgataatt gccactcagc tctacaaata atatttaaat gctcctgaac agtgatgtag
    14401 caagacacgt atggagccaa cgaagaataa aaatttatag agttcttatt caatataacc
    14461 ggaatagtca ggggctgatg taaatgaccg gaatgagtaa gagaggcagc accgcccctc
    14521 tccatcagct actcttcttc gttcccgccc tcttcatacg ggccaaatgg cttcgcgggc
    14581 agaacgatat acgtgccttc aaacactgca cccggcgtct cgtcgccaaa gatttcaact
    14641 tgcatctgca cccgtgcttt tcgtccgcgc gccagacggt cgagatcgcc gcttaaggca
    14701 ccaaggtcgg ctaccgcatg aggtttaccg ctaatcggct tgctgtagcg gatatgcgca
    14761 tccgccagaa taatcgttcc gccgagatgg cgttcgcgca gcatcagcca gataagcccc
    14821 caaccggtga gcgttgccag tgagaataaa ctcccggcaa acagcgtatg gtgcggattc
    14881 tgattaccgg tttccggcat ggtggtgata aatttttgcc cggtatattg ttgaatacgc
    14941 acgcccattt tttcactaag cgggatatgt tcgtaccacg cctgttgcag ctgcgcgcac
    15001 cagtcgccgc gatgcaaaat atcatccaga gtggcaacgg gtttaatcat caaaaaatgg
    15061 cgaatcggcg tggtagttgg ggtggtgatc tccccctgat taatgaatcc cagcttggcg
    15121 aaaaactcca ccgcgtcttc acgggcgcta caggtcacgc gctttacgcc ttcctgacgc
    15181 gccaccgact ccagggtcat cgccatcagc gtgcctaacc ctttgtcctg cacgtccggg
    15241 tgaacggcca taaagcgaat ggacgcttca ttgtcggcat taatatacaa tcggcctacc
    15301 gccaccagat taccctgctc gtcgacgacc atctggtgat gcgccatcgc atcccacgcg
    15361 tcgcgttccg aaccttttgg ttgatgcagg ggcttacgca acatttccca gcgaaactga
    15421 tagtaacgct ctaattcttc ttctgtttgt ggaacccgaa ggtgatacat agcgatactc
    15481 tctcttgttt cccgtgacaa ccctggaagc tggctcatac ctgcaaccag aatgtcacgg
    15541 ggccatcatt gaccagcgat acctgcatat ccgcagcgaa gcgtcctgtt tgcgtgttca
    15601 tctcttgctg acggcagcgt tcgacgaaat agtcatataa cgcctctgcg cgatccggtg
    15661 atgcaccttt ggagaaactt gggcgcatcc cccgttcggt atctgcggcg agggtaaact
    15721 gggaaaccac cagcacactg ccgcccgcct gttgcacgtt gaggttcatc ttgccttcgg
    15781 catcgctaaa gatgcggtag ccgagcacac gctcgcacag acggtttgct ttctgttcgt
    15841 cgtcatcctt ttcgacaccc aataacacca aaagtcccgc gccaatttcg cccgtcactt
    15901 ctccctccac ggtgacgctg gcacgggtta cgcgttgaat taatgcaatc atggttcgtc
    15961 gtcttcttct tgttcagctg cttgttttag tttgcggtat tccccgagag tgacagtaat
    16021 ttccgcgcca agcaagacga tacaccatgt ccagtagacc caaacaaaga gaatggggat
    16081 caccgccaac acgccgtaaa tgagctgata tgacggaaac atggtgatat aaagcgcgaa
    16141 acctttcttt cctgcttcga acaggagcgc ggcgacaaac gcgccgacaa tcgcgtcgcg
    16201 gttaggtacg cggatggtag gaacaatgct gtacagcaac cagaaggaga tccacgacaa
    16261 cagcagcgga aaaatacgca gcacgttatc gatgacagta ttgagatcgc tcgcccagcg
    16321 cagggagagc aaataggaac tgatcgccag actggcccct gccagcagcg gccccagcgt
    16381 taaaatcatc cagtacacgg cgaacgagta aatttttggt cgcgctcgtt tactgcgcca
    16441 gatggtattc aacgcgctat cgatggagta catcagcaat aacgcggtga cgatcagccc
    16501 gcacgcccca acggcggtca tcttgttgga attggcaaca aattgttcga tataccgctg
    16561 aataacatcg ccagtagcag gcagaaagtt ggcaaaaata aagtgacgta actgaatgct
    16621 gacgtcggaa aacatgggaa aagcggcgaa aagcgcaaaa acaacggcaa ccagcggcac
    16681 taatgagagc aacgacacat aggcaaggtt acctgccagg gttgtcatgt tgtcctcatc
    16741 aatgcgttgc cagagtagtt ttagccaggc ccatagtgga cgggtacggt gcctggcttt
    16801 gtcctgaatg gtttttagca taacaccttc gcgaaatagt ccgggatggt ggttttatct
    16861 ttcaccagaa tactggtaat gcccagctga ttggctcctt ctatattatc ggcgttatcg
    16921 tcgaaaaaga ccgtatcgct gggtgaaaaa ccttccgcct gcaaaacatg ctggtaaatt
    16981 cgtgcttcag gtttgcgcat ccccagatct tgcgacagat agatatggtc agcagcatca
    17041 cgaatttccg ggtattcttc cggccagaag gtggtatgca ggcggttggt attggaaagc
    17101 accaccacgc gatgcccctg ctcacgcagt ttatgcatga tggcgatcac ttccgggcgc
    17161 agcgcaacaa acaccgcctg ccagccgtga gagaactgct cgtagcttag cggtagagcc
    17221 atctcatgac acagcgcctc tgcgaacgct tcgtcgctaa tttccccacg ctcatgctga
    17281 tgaaacgcct cccccatatg aaaactcttc ttaagcgatg ccagcggaat acgcgttaaa
    17341 tcgctccagg ctcccagcac acggttaaag tcgatatcga caatcacatt acctaaatca
    17401 aagatataga gcatttttgc ctcctttcgc gccacaagaa attaactgta gcgggaaagg
    17461 atgggtttgg ctatgtggca aggtatgaag ccaggttaac ggtcacaaaa atctacgcgt
    17521 cttcctgggg aagaatgatt tcaatttggc tgtcttgtaa gcgtgatttt agcgcagcgg
    17581 gcggcagctc gtcggtaaag agcgcagtga cctgtgccac gttaccaatt tcaaccgccg
    17641 ccgaagcatg atacttagtg tgatcggcga ccagcagaat atttctcgcg tgcgccatca
    17701 tcgttttcac cacgttagct tcgtttacat caaactccat caacgcgcca tcgctctcaa
    17761 tcgccccaac gcttgttacc agataatcag cacgaaaatc agccacaaag gacgccgctg
    17821 aagggccaat gatcccgcta ttatgagagc gcaacgtacc gccgggcacc atcacttcaa
    17881 agcgcgggtt gtggtaaaga atatgcgcca cacgcaggct gttggtgatt atccgcaaat
    17941 gattatggtt aagtaacgcc cgggcaacat gctcaacagt cgtaccaatg gtgataaata
    18001 ttgttgaacc atcaggaata tagtctgcca ccgcttcggc aatcgctttt ttttcctcgg
    18061 tttgcgaaac ctcacgctgc tcgaacgccg tattaacgac gctggaagcc cgacccgcgc
    18121 caccgtgatg gcgcgtaatc aggccttgct cgcttaattt acgaatatcc cggcggaccg
    18181 tctgcgtgga gacatccagc agatttgcca gctcatcaat attcatatac ccacgctcgg
    18241 cgatcagcat gaggagttgg tcgtgccgcg ggttaccggt tagttcggta aggctcatga
    18301 aaattcctcg aaaaaccatc accctggcat tttatacaaa aagtgacaaa aaagatcggg
    18361 tttgatcaca gtcagggatc ccggctcgtc caccgggacg tgtgcatttt aacgccgcta
    18421 caccgctggc gaagcggact gactccgcta aatccccact tgttgccagc gccaccgcca
    18481 aagcgccgtg aaaaacatca cccgcacctg tggtatctac cacatcaact ttgaaggccg
    18541 gttgatgctg acgcccacca ttttccagcc agtcgcagcc tgcgctaccc tgggtcacat
    18601 agacatgacc atttgtgagc gtttgtgcct gttttagcgc actggccatc tctttcacgc
    18661 ccgttaagcg cgccagaccc ggttctgaaa aggccgcgtg atcgcttaat gccaccagct
    18721 cactgatatc ctgcggcgta atgtccccgt ccagaacggt catcacaccc gcctgacggg
    18781 ccagggtgaa ggctttttta gcgccgtcgt gccagcgtac atctgccagc acaacatccc
    18841 actgagagaa atcaatttcc tccaaccact ctgcgtcagg cagcagatcc gggctggggt
    18901 agttaatgat tatccgctcg cctttggtat ccaccatgat ggcggattgc gaagatttcg
    18961 cctggttata ccgtttggtg taacgggtgt taacgcccca ggattccagc tctgccagca
    19021 ggctgttgcc cgtgtcgtca tcacctacgc gaccaataaa atcgacctgc gcccccagcc
    19081 ttgccgccgc aaccgctgcc gtcgccgctg gcccgccacc aacttccgta taatttctcg
    19141 ccacgtattt accgctctcc gtcggtaacc ctttcacgta atagatgcga tccatcacgg
    19201 ttatacctac acaagcaaca cgaatcatgg tcattcctta aacattttgg gatgcgttca
    19261 ttttaatttc accaattgtt taaaaagtga cggctgtcaa ttttttgacg ataaattaca
    19321 aatacgatca aaaacagaca agcaaagcat gtcataaatg acaaaaagtg acatcactgt
    19381 attcaggaga gggttatggc agcaatcgcg tttatcggtt taggacaaat gggttcgcca
    19441 atggcgagca atttattgca gcaagggcat caacttcgcg tctttgatgt gaacgccgaa
    19501 gctgtgcggc atctggtaga caaaggtgcg actcccgccg ccaacccggc gcaggccgct
    19561 aaagatgccg aatttatcat taccatgttg ccgaatggcg atctggtgcg caacgtgttg
    19621 ttcggtgaaa acggcgtttg cgaaggctta tctaccgatg cgctggtcat tgatatgtcc
    19681 accatccatc cgctgcaaac cgataaattg attgccgata tgcaagccaa aggcttcagc
    19741 atgatggatg ttccggtagg ccgtacttct gcaaatgcca ttaccggtac tctgttactg
    19801 ctggctggcg gcaccgctga acaagttgaa cgtgccacac cgatcctgat ggcgatgggc
    19861 agtgagttga tcaactctgg cggtccgggc atggggatcc gcgttaagct catcaacaac
    19921 tacatgagca tcgcgctcaa tgcgctttcg gcagaagccg ccgttttgtg cgaagccctg
    19981 aatcttccct tcgatgttgc cgtcaaagtg atgagcggta ccgccgccgg taaaggtcac
    20041 ttcaccactt cctggccaaa caaagtcctc agcggagatc tttctcccgc cttcatgatc
    20101 gatcttgccc ataaggatct tggcatcgcc cttgatgtcg ccaaccagct gcatgtgcca
    20161 atgccgctgg gggccgcctc acgggaggtt tatagccagg cgcgcgcagc gggtcgcggt
    20221 cgccaggact ggtccgccat tctggaacag gtccgtgtca gtgccgggat gactgccaaa
    20281 gtaaaaatgt aacgactgga taaaggaata aatgaatgaa taagtacacc atcaacgaca
    20341 ttacgcgcgc atcgggcggt tttgccatgc tggcggtcga tcagcgcgaa gccatgcgca
    20401 tgatgtttgc cgcggctggg gcacccgctc cggtagccga tagcgtttta actgatttca
    20461 aagttaacgc tgcaaaggcc ctctcgcctt atgcctcggc gattctggta gatcaacaat
    20521 tctgctaccg ccaggtggtt gagcaaaacg cgattgccaa aagttgcgcc atgattgtcg
    20581 ccgccgatga gttcattcct ggcaacggta ttccggtcga tagcgtggtt attgaccgca
    20641 aaatcaatcc gctacagatc aaacaagacg gtggcaaagc cttaaaactg ctggtgctgt
    20701 ggcgtagtga tgaagatgcg cagcaacgtc tggatatggt gaaagagttc aacgaactgt
    20761 gccactcaca cggtctggta agcatcattg agccagtcgt ccgcccaccg cgtcgtggcg
    20821 ataaattcga tcgcgaacaa gcgatcatcg atgccgccaa agagctgggc gacagtggcg
    20881 ctgacctcta caaagttgaa atgccgcttt atggcaaagg tccgcaacaa gagcttctct
    20941 gtgcttcaca acgtctgaat gaccatatca atatgccatg ggtgatcctc tcttccggtg
    21001 tcgacgaaaa actgttcccg cgtgccgtac gcgtggcaat gacggcgggc gcatcgggat
    21061 tcctggcagg ccgtgcagtc tgggcatcgg tcgtcggttt accagacaac gagctgatgc
    21121 tgcgtgacgt ttgcgcaccg aaattacaac aacttggcga tatcgtcgac gaaatgatgg
    21181 ctaaacgccg ctaagatgag gataaaagga atgaaatggt ttaacaccct aagccacaac
    21241 cgttggctgg aacaggaaac cgaccgcatc tttgattttg gtaaaaattc cgtagtgccg
    21301 actggttttg gctggttagg caataaaggg caaatcaaag aagagatggg cacccatctg
    21361 tggatcaccg ctcgtatgtt gcacgtttat tccgttgctg cggcgatggg tcgacctggc
    21421 gcttactcgt tggttgatca cggtatcaaa gccatgaacg gcgcactgcg tgataaaaaa
    21481 tatggcggct ggtatgcctg cgtgaatgac gagggcgtgg tggatgcctc caaacagggc
    21541 tatcaacact tctttgctct gctgggtgcc gccagcgccg tcacaacggg tcacccggaa
    21601 gcgcgcaagc tgctcgatta caccattgaa attatcgaga aatatttctg gagcgaagaa
    21661 gagcagatgt gcctggaatc ctgggacgaa gccttcagca aaaccgaaga gtaccgcggc
    21721 ggcaatgcca atatgcacgc ggtggaagct ttcttgattg tttatgacgt cactcacgac
    21781 aaaaaatggc tggatcgcgc gattcgcgtg gcttccgtga ttatccacga cgtcgccaga
    21841 aataatcatt atcgcgttaa cgaacacttc gatacccagt ggaatccgct gccggattac
    21901 aacaaagata acccggcgca tcgcttccgc gcgtttggag gtacaccagg ccactggatc
    21961 gaatggggcc gtttaatgct gcacatccat gctgccctgg aagcccgttg cgaacaacca
    22021 ccagcatggc tgctagaaga tgccaaaggt ctgtttaacg ccaccgtgcg cgatgcctgg
    22081 gcacccgatg gcgcggacgg gattgtttat accgttgact gggaaggaaa accggtggtc
    22141 cgcgaacgtg tacgttggcc tatcgtcgaa gcaatgggta cggcctacgc gctctacacc
    22201 gtcactggcg atcgccagta tgaaacctgg tatcaaacat ggtgggagta ctgcattaag
    22261 tacctgatgg actatgaaaa tggttcctgg tggcaggagc tggatgcgga caataaggtc
    22321 accaccaaag tctgggacgg caaacaggat atttatcacc tgctgcattg cctggtgatc
    22381 ccgcgtatcc cgttagcgcc cggcatggct ccagcggttg cagcgggtct gctggatatt
    22441 aatgcgaaat aactgatctg aaaatagggt gcgggttttc ccctcaccct aaccctctcc
    22501 ccaaaggggc gaggggaccg tccggtgcag tttttgggtt tgacatcagc ctcacgggag
    22561 gctgtatgtc gttaattaag gtgccttgta tgcaaatcac caatatgcat tgcagtggac
    22621 agaccgtttc acttgccgcg ggggattatc acgcgaccat tgtgaccgtc ggtgctggtc
    22681 tggctgaatt aacctttcag ggttgtcatt tagtcatccc gcacaaaccc gaagagatgc
    22741 cgctggcgca tctgggtaaa gtgcttattc cgtggccgaa ccggatcgcc aacggctgtt
    22801 accgctatca ggggcaggaa tatcagttac ccattaatga acatagttca aaagccgcta
    22861 ttcacgggct gcttgcctgg cgagactggc aaattagtga attaaccgcc accagcgtta
    22921 ccctgacagc ttttctgccg cccagttacg gctatccgtt tatgctggct tcacaagttg
    22981 tttattcgct caacgcacac acgggtcttt ccgttgagat cgcaagccag aatatcggca
    23041 ctgtcgcagc gccttatggc gtgggtattc atacttattt aacctgcaac ttaacatctg
    23101 ttgatgaata tctgttccag cttccggcaa accaggttta tgccgttgat gaacacgtaa
    23161 atccgacaac gcttcaccac gttgatgagc tggatttaaa tttcacgcag gcgaaaacaa
    23221 tagccgcgac aaaaatcgac cacaccttta aaaccgctaa tgatctgtgg gaaataacca
    23281 ttacccatcc acaacaggcg ctttcggtta gcctttgttg cgatcaaccc tgggtacaaa
    23341 tttatagcgg tgaaaaatta cagcgtcagg gactggcagt tgaacctatg agttgcccgc
    23401 ctaacgcctt taattcaggt atcgacttat tattactgga accaggaaaa ccgcaccgcc
    23461 tgttttttaa catttacgga cagagaaaat aacttgtatt gattagcagt caaaatcatt
    23521 gaagaacatt tcactcgcta aaggcaacat tatgtatctt caatttatta agttaaaaac
    23581 agctcagctt ctgtaaagtt tttatatatc agttttatga gaatcatttt acataagcct
    23641 gtaataacag gaaggcaaca atctcgttct caactctatg gagattagtt atggatacgc
    23701 cacgtccaca gttattagat tttcaatttc atcagaataa cgacagtttt accctacatt
    23761 ttcaacaacg tcttatttta acccatagca aagataatcc ttgtttatgg attggctcag
    23821 gtatagcgga tatcgatatg ttccgcggta atttcagcat taaagataaa ctacaggaga
    23881 aaattgcgct taccgacgcc atcgtcagcc agtcaccgga tggttggtta attcatttca
    23941 gccgtggttc tgacattagc gccacgctga atatctctgc cgacgatcag ggccgtttat
    24001 tgctggaact acaaaacgac aaccttaacc acaaccgtat ctggctgcgc cttgccgctc
    24061 aaccagagga ccatatctac ggctgcggcg aacagttttc ctacttcgat ctgcgtggca
    24121 aaccgttccc gctatggacc agtgaacaag gcgttggtcg caacaaacaa acctatgtca
    24181 cctggcaggc cgactgcaaa gaaaatgcgg gcggcgacta ttactggact ttcttcccac
    24241 agcctacgtt tgtcagcacg cagaagtatt actgccatgt tgataacagt tgctatatga
    24301 acttcgactt tagtgccccg gaataccatg aactggcgct gtgggaagac aaagcaacgc
    24361 tgcgttttga atgtgctgac acatacattt ccctgctgga aaaattaacc gccctgctgg
    24421 gacgccagcc agaactgccc gactggattt atgacggagt aacgctcggc attcagggcg
    24481 ggacggaagt gtgccagaag aaactggaca ccatgcgtaa cgcgggcgtg aaggtcaacg
    24541 gcatctgggc gcaggactgg tccggtattc gtatgacctc ttttggcaaa cgcgtgatgt
    24601 ggaactggaa gtggaacagc gaaaactacc cgcaactgga ttcacgcatt aagcagtgga
    24661 atcaggaggg cgtgcagttc ctggcctata tcaacccgta tgttgccagc gataaagatc
    24721 tctgcgaaga agcggcacaa cacggctatc tggcaaaaga tgcctctggc ggtgactatc
    24781 tggtggagtt tggcgagttt tacggcggcg ttgtcgatct cactaatcca gaagcctacg
    24841 cctggttcaa ggaagtgatc aaaaagaaca tgattgaact cggctgcggc ggctggatgg
    24901 ctgacttcgg cgagtatctg cccaccgaca cttacctgca taacggcatc agcgccgaaa
    24961 ttatgcataa cgcctggccc gcgctgtggg cgaaatgtaa ctacgaagcc cttgaagaaa
    25021 cgggcaagct cggcgagatc cttttcttta tgcgcgccgg ttctaccggt agccagaaat
    25081 actccaccat gatgtgggcg ggcgaccaga acgtcgactg gagtctcgac gatggcctgg
    25141 cgtcggttgt cccggcggcg ctgtcgctgg caatgaccgg acatggcctg caccacagcg
    25201 acattggcgg ttacaccacc ctgtttgaga tgaagcgcag caaagagctg ctgctgcgct
    25261 ggtgcgattt cagcgccttc acgccgatga tgcgcaccca cgaaggtaac cgtcctggcg
    25321 acaactggca gtttgacggc gacgcagaaa ctatcgccca ttttgcccgt atgacctccg
    25381 tcttcaccac cctgaaacct tacctgaaag aggctgccgc gctgaatgcg aagtccggcc
    25441 tgccggttat gcgcccgctg ttcctgcatt acgaagacga tgcgcacact tacaccctga
    25501 aatatcagta cctgttaggt cgcgacattc tggtcgctcc ggtgcatgaa gaaggccgta
    25561 gcgactggac gctctatctg ccggaggata actgggtcca cgcctggacg ggtgaagcgt
    25621 tccggggcgg ggaagttacc gttaatgcgc ccatcggcaa gccgccggtc ttttatcgcg
    25681 ccgatagcga atgggcggca ctgttcgcgt cgttaaaaag catctaatca catccgcccg
    25741 tggggaaact catgggcaaa gggagaacat cgatgagtca catcacaacg gaagatccag
    25801 caactttacg cctgcccttt aaagagaaac tctcttacgg tattggcgac ctggcctcta
    25861 acatcctgct ggatatcggt acactttatc ttttgaagtt ttataccgac gttctggggc
    25921 tgccaggcac ctatggcggc attatctttt tgatttcaaa attctttact gcgtttaccg
    25981 atatgggtac cggcattatg ttggattccc gacgcaagat cggtccaaaa ggtaagttcc
    26041 gtccttttat tctgtatgcg tcattcccgg tcaccttact ggcgatcgcc aactttgtcg
    26101 gcacaccgtt tgatgtcacc ggtaaaacgg tgatggccac tattctgttt atgctttacg
    26161 gactgttttt cagcatgatg aactgctcct acggcgcaat ggttcccgct atcaccaaaa
    26221 accccaacga acgcgcctca ctggcggcat ggcgtcaggg cggcgctacg ctgggcctgc
    26281 tgctgtgcac ggtgggattc gtgccagtga tgaatcttat cgagggtaat cagcaacttg
    26341 gctatatctt cgccgccacg ctgttttcac tgttcggcct gctgtttatg tggatctgct
    26401 actcaggcgt gaaagagcgt tatgtcgaaa cccaaccaac caatccggcg caaaagcctg
    26461 gcctgttgca atctttccgc gcaattgccg gtaaccgccc actgttcatt ctgtgtattg
    26521 ccaacctctg caccttaggg gcgtttaacg tcaagctcgc cattcaggtc tattacaccc
    26581 agtacgtact taacgatccc atcctgttgt cgtatatggg atttttcagc atgggctgta
    26641 ttttcatcgg cgtgttcctg atgcctggcg cagtcagacg ttttggtaag aagaaggtct
    26701 atatcggcgg cctgctgatt tgggtgctgg gcgatctgct caactatttc ttcggcggcg
    26761 gctcggtcag ctttgtggcg ttctcctgcc tggcgttctt cggctcagcg tttgttaaca
    26821 gcctgaactg ggcgctggtt tccgacaccg tcgagtacgg cgagtggcgc accggcgtgc
    26881 gttcggaagg cacggtctac accggcttca ccttctttcg caaagtgtct caggcgctgg
    26941 ctggtttctt ccccggctgg atgctgacgc aaattggcta tgtgccgaac gtcgcacagg
    27001 ctgaccacac cattgaaggg ttacgccagt tgatcttcat ctacccaagc gcactggcgg
    27061 tagtcaccat tgtggcgatg ggttgcttct acagcctgaa cgagaagatg tatgtccgca
    27121 ttgtggaaga gatagaagca cgtaaacgca cggcgtaata ataataaacg acgccctgcg
    27181 gggcgttata aggagtgatt atgtctgacc ataatccact gacattaaaa ctgaatctgc
    27241 gggaaaaaat cgcctatggt atgggcgacg tcggttcgaa tttaatgctc tgcatcggta
    27301 ctctgtatct cctcaaattt tataccgatg aattagggat gcctgcttac tatggcggga
    27361 ttatctttct ggtggcgaag ttttttaccg cgtttaccga tatgctcacc ggatttttac
    27421 tcgactcgcg gaaaaatatt gggcctaaag gcaaatttcg tccctttatt ttatatgcag
    27481 cagttccggc ggcgttaatt gcaacgctcc agtttatcgc caccaccttt tgtttgccgg
    27541 ttaaaacgac gattgccacc gcgctgttta tgatgtttgg actttcatac agtctgatga
    27601 actgctcgta tggtgcgatg atcccggcaa ttaccaaaaa cccgaatgag cgcgcgcagc
    27661 tcgcggctta ccgccagggt ggtgcaacca tagggctatt gatttgtacc gtggcgttta
    27721 ttccgttgca gtcgcttttt tctgactcaa ccgtcggtta tgcctgtgcg gcacttatgt
    27781 tctccattgg cggctttatt tttatgatgc tgtgctacag aggcgtcaaa gagcattatg
    27841 tggacacagc gccaaccgga cataaagcca gtattctcaa atctttttgc gcgatatttg
    27901 gtaatccacc attgctggtt ttatgcattg ctaacctgtg tactctggcg gcatttaata
    27961 tcaaactggc gattcaggtc tattacaccc agtatgtgct gaatgatatt aatttattgt
    28021 cgtggatggg attcttcagt atgggatgca tcctcgtcgg cgtattactg gtgccagtaa
    28081 ctgtaaaatg ttttggtaaa aaacaggttt atttagctgg catggtgctg tgggcggtgg
    28141 gtgatatact gaattatttc tggggaagta actctttcac tttcgtcatg ttctcttgtg
    28201 tcgccttttt tggcacggcc tttgtaaaca gcctgaactg ggcactggta ccagataccg
    28261 tagattacgg tgaatggaaa accggtattc gtgccgaagg ttctgtttat accggttata
    28321 ccttctttcg taaaatttct gccgcacttg ctggcttctt gccaggcatt atgctgacgc
    28381 aaattggtta tgttcccaac atcgcgcaaa gcgatgcgac attacagggt ttgcgtcagc
    28441 tcatttttat ctggccttgt gcactggcaa ttattgctgc attgacgatg ggattctttt
    28501 acacactcaa tgaaaaacgg tttgcattga ttattgagga aatcaaccaa cgcaaaaata
    28561 aagagatggc gacagaagaa aaaacggctt ccgtaacgtt ataaatgtca atagccgcta
    28621 tttccatcct ggtgggtggc ggcctcccta cgtttaaaaa atggacttat tatgaaaaag
    28681 attaatgcaa taattttatt atcttctctg acctctgcct cggtatttgc tggcgcatac
    28741 gtcgaaaaca gggaggcata taatcttgcc tccgatcagg gcgaggttat gctacgtgtg
    28801 ggttataact tcgatatggg cgcgggtatt atgttaacca atacctacac ctttcagcga
    28861 gaagatgaac taaaacatgg ctataatgaa attgaaggtt ggtatccttt attcaaacca
    28921 accgataaat taaccatcca gcccggtggc ttaattaatg ataagagtat cggttcgggt
    28981 ggtgcagtgt atctggacgt caactataaa tttgtaccat ggtttaatct gacagtacga
    29041 aatcgctata accataataa ctatagttca acagatttga gcggggaact ggataataat
    29101 gacacctatg aaattggcac ctactggaat tttaaaatca ccgataaatt ttcctataca
    29161 tttgagccac attatttcat acgcgtaaat gactttaata gcagcaatgg aaaagatcat
    29221 cactgggaaa tcactaatac cttccgttat cgcattaatg aacactggct gccttatttc
    29281 gaattacgtt ggttagatcg taatgtcgaa ccgtaccacc gcgagcaaaa ccagattcgt
    29341 atcgggacga agtatttctt ctgatgtgaa actgccggat gcggcgtaaa cgccttatcc
    29401 ggcctacggt tgatacggtt ttcgttggcc ggattaagca catccggcca ttaatcaagc
    29461 ttcagccgcc agcatctctt tcttacgttg gctaattcgc ttaaccagca ttacggaaac
    29521 caccgcacca cagaatgcga agcaggccat aatgccaaac acgattttgt agccaataat
    29581 ccccggattt aaatccagaa tgtagccata cagactgaag cagaacatcg ccggagcgta
    29641 accaataaag ctacccaacg ccatcgccgc gcctgtttta ttttcagcaa tttttgcttc
    29701 gccgataggt gcaaaaaata cggctcgctg tgtaaagact atcgcgccaa agcccagcgt
    29761 acatgccatc cctaaataga ccggcatact ttcgtgcggc agcataatca acagtacgag
    29821 cgcagcggta ctgatgataa aggtgtagca tagatattta ctcggcgatt tcaggatctt
    29881 atctgaaatc atgccaccaa tcggtccacc aatcattttc agacagtatt ggttaatgat
    29941 gccgtaagcc cccaccagcg caacgggcaa tagatagatg tttttcagga atggaatgaa
    30001 gaatgtcagg ccacagtaaa ccgcgtaaac aaagaagacg ttaaaagcga taagccagat
    30061 ggttttgtct ctcagcaccg agatcatact ggtgttttgc gatgctccgt cttctttttt
    30121 cacctcaacg gacggtgcct cttctttgtc attcaggaca aagaaaataa taatgcctac
    30181 ggcaatcaca ataagggaat agaaccagat gcctgcttta aaacctaata agccactgcc
    30241 aaaccaggta aatactgcca acgcagaaaa tgccaccacg gtatcgacaa tgccacgccc
    30301 tgtttcgaag aagccaaaca accgaccttg ttgttcgcta ttgcccaatc gacttaccga
    30361 cttgagcaag accggccagt tcatcatgtc gcaagtaacg ccaaatagcg cccagacaaa
    30421 gaggatgccc caataccccg gcattgtcgt caaataaaca cccagtaatc ctgtcgcaat
    30481 gagtgagaat gacatggtgt atctgcgcgg tagtttatcg gcaaaataaa tagacagaaa
    30541 aaagcccact gtggtgacaa atgagtttac cgacatagca ttaccaattt gaccattggt
    30601 caaatggaaa tattcctgca tcgggatata aaacgcatct ttcagcgacg gtaatttata
    30661 aattgtaccg ccacacagtg ttaatagact aaataacgcc catttctttt tcgtgagcat
    30721 aacatcccct aagcgatttt taaaatcact gttttaaagg caataagata ccgaccattc
    30781 atatagaatg gttttattaa ctgcgtatga gttaaaactt attagtggtt cagaagatgg
    30841 tttgcctctt gtttgatttg ttgtagtacg ttacgcacat gatttatctg attaattgca
    30901 tcttcttttt ctttacgtaa ccgcgcatcc agtaaatgat tttctggtag gggtgtttca
    30961 ctcatctggc gataagggat ccacggatta tcatcttcgt cttcaaagcg gaacgccggc
    31021 gcatagtaat caacctcttc ttccaggcca tatgccgtca cctgcggctc atcatcacca
    31081 aggcagataa ggtgcgttaa taacgctttg aacggcatat caccctgacc tgaaatacag
    31141 gctttatgac ccaggccacc ctgttcttta acgatcaagg catctttaat atggacctgg
    31201 gtaatatgcg gtgccatcgt ttttaaggcg tcgatgggat gctcatttgc attgatcatg
    31261 ttcgcaaaat caaacagtaa ggaaagagac tccatctcac tttctttgac cagcgacacc
    31321 aactcatgac tttttaaatc ttcatgctgc tcgatcgtaa aagtcaggcc gctgtcctga
    31381 tacgtttccc gtacataggc aatgtcgtta gcgataatcg ataatacgtc gcgcaaatta
    31441 ccttcataac gtggataaaa acgtacggac gatgccccag ttttcaacgc gatggcgacg
    31501 gcttcgtcaa tagatgcctt atctgaggcg ctggtttcaa tatgaatatc aaggttcaga
    31561 cggcgggctt tatccccaaa ggcagagagt tctttatcgt ccatattacc aagagaaaaa
    31621 cgctcgccat ccagaacatg gattttgacg ccacgtagat tattttcact tgcgatatca
    31681 agtaaatcag caggcaacac tctttccagc cgcatattta aatgaaaggc ataagcatgt
    31741 aaataaagag gaagagtgtc gacacgttgt agaatctttc ttgcgttatt tattgtcacc
    31801 ataaaacacc tacagattaa tgatgaggtc tacggcctca aatcgccaat attcaatatc
    31861 tatctggaca atattgcctt gctggtcggc gcgatgcttt tcaattaaga tcgctggcat
    31921 acccgcagaa ccgccaagat acttgctgat atcgcccggc attctgacgg gtttgaaaat
    31981 caattttttt actaccgtct ctttttggta gcacttttcc cagactgcag aaaatgagtg
    32041 gttttcaagt tgttcaataa aacccggagc aacttcagga ttaatatatg tttcgtgata
    32101 gaaaacttta tgtccttcca gcgcccccca gccggtgatg cgatagagtt cagttgatgg
    32161 tgtcactgca atcagtggcg cgagcgtctt gggaatatcg ctggtacggt ttttctcggt
    32221 aaacccccag gaaggctctc gtccttgctc aattgcggcc cgctgaaagc tcgccgacaa
    32281 ctccggactg taattaaatc gcggctgggt cacgaaccac cccttacgat ccttacggaa
    32341 gattttggat tctgcctcga ggttcaacaa cgcctggcgc agcgtcatac gtttaatgcc
    32401 cagcagttct cccagctctc tttctgaagg gagttttcca cccggcgtgg tgatgccatc
    32461 tttcaaccac cgatccagtt tctcttttgc attttctacg gtggattgat tctccgccat
    32521 tgtttaatcc ttttggttta aaccagatga agcatcttac gcttacgcca aaaaagaaag
    32581 caattaacgg cgtatgactt tgcagataat tgtgacaaag ctctctattg acgtaatccc
    32641 agacttataa gggagatact cgcattggtg tgcgctcacg acaggtaaaa aaaaacctgc
    32701 cagcgatggc aggtttttta taactaaaaa acgaaattaa tcgtctttcg gtgcgcggtt
    32761 ggcgcgacga cgatcgtttt cagtcaggtg acgtttacga atacggatag aggtcggagt
    32821 cacttctacc agttcgtcat catcgatgaa ctccagagct tgttccagag tcatgcggat
    32881 aggcggaacc agaacaacgg cttcgtcagt accggaagca cgcatgttgg tcagtttctt
    32941 accagtcagg cagtttaccg tcaggtcgtt agagcggctg tgaataccga taatctgacc
    33001 ttcgtaaact tctgcaccgt gaccgaggaa cagcttaccg cgatcctgca gaccgaacag
    33061 cgcgaacgcg accgctttac cctgaccgtt agagatcagt acgccgttct gacgctgacc
    33121 cacttcaccc ggacgtacgt cgtcgtagtg gctgaaggtg gagtacagca gaccagtacc
    33181 ggaagtcatg gtcatgaact cagaacggaa gccaatcaga ccacggcttg ggatcacgta
    33241 gtcgagacgt acgcggcctt taccgtctgg attcatgttt ttcaggtcgc ctttacgttc
    33301 gcccagcgcc tgcattacag aaccctgatg ctgttcttca acgtccagag tcacgttttc
    33361 atacggctct tgtttacgac cgtcgatttc acggaagata actttcggac gggataccgc
    33421 cagttcgaaa ccttcacgac gcatgttttc gatcagaaca gacaggtgca gttcgccacg
    33481 accagaaacg cggaacgcat cggcgtcttc ggtttcttct acgcgcagcg caacgttgtg
    33541 taccagttct ttgttcagac gatccaggat ctgacgagac gttacgaact taccttcttt
    33601 accgcagaac ggcgaggtgt taacgcagaa gaacatagaa acggtcggct catcaacgga
    33661 gagtgccggc agcgcttcaa cgttttgcgt gtcgcaaacg gtgtcagaaa tgttcagttc
    33721 gccaaggccc gtgatcgcaa cgatatcgcc agcttccgcc agatcggttt cgatacgttc
    33781 cagaccgagg tggcccagca ctttaccgac tttcgcgttg cgggttttgc cttcgctatc
    33841 gatgatagtg acctgctggt tcggcttcac tttaccgcgc ttgatgcggc caatgccgat
    33901 aacgccaaca tagctgttgt aatcgagctg agaaatctgc atctggaacg gaccgtcaag
    33961 gtcaacgtcc ggcgcaggaa cgtgatcaac aatcgcctga tacagcgggg tcatgtcttc
    34021 cgccatatct tcgtggtcca gacccgcgat accgttcagc gcagaagcgt aaacgatcgg
    34081 gaagtccagc tgctcgtcgg tcgcgtcgag gttaacgaac agatcgaata cctgatccac
    34141 aacccaatca ggacgcgcgc cagggcggtc aactttgttg ataacaacaa tcggcttcag
    34201 gccgtaagca aacgcttttt tggttacgaa gcgcgtttgc ggcatcgggc cgtcaaatgc
    34261 gtcaaccacc agcagcactg agtctaccat ggacattaca cgttcaactt caccaccgaa
    34321 gtcggcgtgc cccggggtat caacgatgtt gatacggtaa tcattccatt tgatagcggt
    34381 gtttttcgcg aggatggtaa tcccacgctc tttctccaaa tcgttggagt ccatcacgcg
    34441 ctcttgggtt tcggcacgag agtcgaacgt accggattgt tggagcagct tgtctaccag
    34501 ggtggtttta ccatggtcta cgtgcgcgat gatggcgata ttacgcaatt tttcgatcac
    34561 aactttgcct caggcattag aaatagcgcg ttattgtaca cggattaatc gcactacaaa
    34621 acaggatcac aaacatcctc cgcaaacaag tattgcagag tccctttgtg atcgctttca
    34681 cggagcataa aaagggttat ccaaaggtca ttgcaccaac atggtgctta atgtttccat
    34741 tgaagcacta tattggtgca acattcacat cgtggtgcag cccttttgca cgatggtgcg
    34801 catgataacg ccttttaggg gcaatttaaa agttggcaca gatttcgctt tatctttttt
    34861 acggcgacac ggccaaaata attgcagatt tcgttaccac gacgaccatg accaatccag
    34921 gagagttaaa gtatgtccgc tgaacacgta ctgacgatgc tgaacgagca cgaagtgaag
    34981 tttgttgatt tgcgcttcac cgatactaaa ggtaaagaac agcacgtcac tatccctgct
    35041 catcaggtga atgctgaatt cttcgaagaa ggcaaaatgt ttgacggctc ctcgattggc
    35101 ggctggaaag gcattaacga gtccgacatg gtgctgatgc cagacgcatc caccgcagtg
    35161 attgacccgt tcttcgccga ctccaccctg attatccgtt gcgacatcct tgaacctggc
    35221 accctgcaag gctatgaccg tgacccgcgc tccattgcga agcgcgccga agattacctg
    35281 cgttccactg gcattgccga caccgtactg ttcgggccag aacctgaatt cttcctgttc
    35341 gatgacatcc gtttcggatc atctatctcc ggttctcacg ttgctatcga cgatatcgaa
    35401 ggcgcatgga actcctccac ccaatatgaa ggtggtaaca aaggtcaccg tccggcagtg
    35461 aaaggcggtt acttcccggt tccgccggta gactctgctc aggatattcg ttctgaaatg
    35521 tgtctggtga tggaacagat gggcctggtg gttgaagccc atcaccacga agtagcgact
    35581 gctggtcaga acgaagtggc tacccgcttc aataccatga ccaaaaaagc tgacgaaatt
    35641 cagatctaca aatatgttgt gcacaacgta gcgcaccgct tcggtaaaac cgcgaccttt
    35701 atgccaaaac cgatgttcgg tgataacggc tccggtatgc actgccacat gtctctgtct
    35761 aaaaacggcg ttaacctgtt cgcaggcgac aaatacgcag gtctgtctga gcaggcgctg
    35821 tactacattg gcggcgtaat caaacacgct aaagcgatta acgccctggc aaacccgacc
    35881 accaactctt ataagcgtct ggtcccgggc tatgaagcac cggtaatgct ggcttactct
    35941 gcgcgtaacc gttctgcgtc tatccgtatt ccggtggttt cttctccgaa agcacgtcgt
    36001 atcgaagtac gtttcccgga tccggcagct aacccgtacc tgtgctttgc tgccctgctg
    36061 atggccggtc ttgatggtat caagaacaag atccatccgg gcgaagccat ggacaaaaac
    36121 ctgtatgacc tgccgccaga agaagcgaaa gagatcccac aggttgcagg ctctctggaa
    36181 gaagcactga acgaactgga tctggaccgc gagttcctga aagccggtgg cgtgttcact
    36241 gacgaagcaa ttgatgcgta catcgctctg cgtcgcgaag aagatgaccg cgtgcgtatg
    36301 actccgcatc cggtagagtt tgagctgtac tacagcgtct aatagttgaa gttgtactac
    36361 ccggcgcaac aacgccggag gttcaaacca ggcccatctg gcgtaattgt tgcagccagt
    36421 ttgaacacgg acagcgcgca gaacccggag cgtacactgg tacgtgagga gttcgagcac
    36481 tgcccaggtt caaaatggca aataaaatag cctgatggga ctggttttta gttgccgtgg
    36541 aaatttcagc ccatctctgc atgggctttt ttctccgtca attctctgat gcttcgcgct
    36601 ttttatccgt aaaaagctat aatgcactaa aatggtgcaa cctgttcagg agactgcttt
    36661 atggcaacag gcacgcagcc cgatgctggg cagatcctca actcgctgat taacagtatt
    36721 ttgttaatcg atgacaacct ggcgatccat tacgccaacc ctgccgcgca acaactgctc
    36781 gcccaaagct cccgcaaatt gtttggtaca ccgttaccgg aactgttgag ctacttctca
    36841 ttaaatatcg agctgatgca agaaagtctg gaggcggggc aaggttttac cgataacgaa
    36901 gtgacgctgg tcatcgacgg gcgctcgcat atcctttctg tgacggccca gcgtatgccg
    36961 gacggcatga tcctgctgga gatggctccg atggataacc agcgccgctt aagtcaggaa
    37021 cagctacagc acgcccagca ggttgctgcc cgtgatttag tgcgcggcct ggcacatgag
    37081 attaaaaatc cgcttggcgg tttacgtggc gcggcgcagc tactcagcaa agcgttacct
    37141 gacccatcac tactcgaata taccaaagtg attatcgaac aggcggaccg gctgcgaaat
    37201 ctggtcgacc gtctgttggg gccgcagctg cccggtacgc gcgttaccga aagtattcac
    37261 aaagtagctg aacgcgtggt aacgctggtg tcgatggaac tgccggacaa cgtgcggttg
    37321 attcgtgatt acgatcccag cctgccggaa ctggcgcacg acccggatca aattgaacag
    37381 gtcttgctga atattgtgcg caatgcgcta caggcgctgg ggccggaggg tggtgaaatc
    37441 attctgcgta cccgcaccgc gtttcaactg accttacacg gcgagcgcta ccggctggcg
    37501 gcgcggattg atgtggaaga taacgggccg ggcattccgc ctcatttgca ggatacgctg
    37561 ttttacccga tggtcagcgg ccgcgaaggt ggcaccgggc ttggcttatc catcgcccgt
    37621 aatttgattg atcagcattc aggcaaaatt gaatttacca gttggccagg tcataccgag
    37681 ttctcggttt acctgcctat caggaaataa aggtgacgtt tatgcaacga gggatagtct
    37741 gggtagtcga tgacgatagt tccatccgtt gggtgcttga acgtgcgctc gctggagcgg
    37801 gtttaacctg tacgacattt gagaacggcg cggaagtact ggaggcgctg gcgagcaaaa
    37861 cgccggatgt gctgctttca gatatccgta tgccgggaat ggacgggctg gcgctgctca
    37921 agcagattaa acagcgccat ccaatgcttc cggtcatcat tatgaccgca cattccgatc
    37981 tggatgctgc cgtcagcgcc tatcaacaag gggcgtttga ttatctgccc aaaccgtttg
    38041 atatcgacga agccgtcgcg ctggttgagc gcgccatcag tcattaccag gaacagcagc
    38101 agccgcgtaa tattcagctt aacggcccaa cgaccgatat catcggcgaa gcgccagcca
    38161 tgcaggacgt gttccgtatt atcggtcggc tttcgcgttc ttctattagc gtgctgatta
    38221 acggcgaatc cggcaccggt aaagaactgg tcgctcatgc cctgcatcgc cacagtccgc
    38281 gcgccaaagc gccgtttatc gcgctgaata tggcagctat cccaaaagat ttgatcgaat
    38341 cagaactgtt tggccacgag aaaggcgcgt ttactggcgc gaatactatt cgtcaggggc
    38401 gttttgaaca ggccgatggc ggtacattat tcctcgacga aattggtgat atgccgctgg
    38461 atgtgcagac gcgtttgctg cgcgtgctgg cagacggtca gttttaccgc gttggcggct
    38521 atgcgccggt gaaagtggat gtgcggatta tcgctgccac tcaccagaat ctcgaacagc
    38581 gagtgcagga aggtaagttc cgtgaggatc tgttccaccg cctgaacgtt atccgcgttc
    38641 atctgccgcc gctgcgcgaa cgtcgggaag atattccccg tctggcgcgc cattttttac
    38701 aggttgccgc gcgcgaactg ggcgtagaag cgaagttact gcatccggaa accgaagctg
    38761 ctctgacgcg tctggcgtgg ccaggcaacg tgcgccagct ggaaaacacc tgccgctggc
    38821 taacggtgat ggccgccggg caggaagtgt tgattcagga tttgcccggc gaactgtttg
    38881 aatcaacggt tgcggagagt acttcgcaaa tgcaaccgga cagctgggcg acgcttcttg
    38941 cgcagtgggc agacagagcg ctgcgttccg gtcatcaaaa tctgctttcc gaagcgcagc
    39001 cagagctgga gcggacgtta ctgacgaccg cgttgcgaca tacgcagggg cataaacagg
    39061 aagcggcgcg gctactcggc tggggccgca acaccctgac gcgtaagtta aaagagctgg
    39121 ggatggagtg attcacggcg tgtgtgtaaa gattgattat tgagcgcaaa ttgctggtat
    39181 tttacgcttt actgttccga taagttcagt atgatcttgc ccggaaaacg gggagagtca
    39241 ttatgctgga atcaataatt aatctggtat cgagtggcgc agttgacagc cacacaccgc
    39301 aaactgccgt tgctgccgtg ctgtgtgccg caatgattgg gctgtttagt tgagtttcag
    39361 attgctgatg aagtgctctt tgttcatgcc ggatgcggcg tgaacgcctt atccggccta
    39421 caaaagcaat attttcaaca ggttttgtag gcctgataag cgtagcgcat caggcaatct
    39481 tgttctcagc acattgatgg cgctttcgtt ttctactttg taaacgaagc gccattcact
    39541 atttaaatca cccgtgagaa ctgctgcatc cgcgctttct ggcgcagata ggtatcaaag
    39601 cacatgcaaa tgttgcggat cagcaagcga cctttcgccg tcacctgtat tcccttctca
    39661 tccacatcca ccagcccatc ttttgctaac ggggcgagca gcttgagatc ttccgcaaag
    39721 taatcagcga agtgcaaatc ccactgtttc tcaatagggg cgtaatccag acggaagttg
    39781 cagatgagcg acttaatcac atcgcggcga atacagtcat cacgcgttag cgcaatacca
    39841 cgccacagcg cattgccttg ttcatccact tgctgatagt actgcttcaa ctctttctgg
    39901 ttctgcgcgt agcagtcgcc aatcatgctg atggcggaaa cgcccatccc cagcagatcg
    39961 gtatcgccct gagtggtgta gccctggaag ttacgatgca gcacgccttc acgctgggcc
    40021 accgccagct cgtcatccgg acgggcaaag tgatccatac cgataaactg atagcccgat
    40081 tgcgtcagga aggcgatggt ttcctgcagg atatcgagtt tttgctgcgg actcggcagg
    40141 tcagcatctt tgattttgcg ctgagcagca aaaatggtcg gcagatgcgc gtagttaaag
    40201 acactcagac gatcggggtt cagctccgcc acacgtttca gggtaaaggc gaaactctcc
    40261 ggcgtctgtt tcggcaggcc gtaaatcagg tcgatgttgg tggaggtaaa accaatctca
    40321 cgcgcatggt taagcagtgc aaagatgaac tcttcatcct gctcgcggtt aaccagacgt
    40381 tgcacttctt tgttgaagtc ctgcacgccc atgctcaggc gattaaagcc ttcggcgcgt
    40441 aaatgatcga gtacatccag ttcgatttcc cgcggatcga cttcgatcga aatctccgca
    40501 tcggcattga actggaagtt ttcgcgcagc agcttcatca ggcggctgat ttgcgcttta
    40561 ttcagatacg tcggcgttcc gccgccccag tgcaattggc tgacgtgacg cccggcgaac
    40621 agcggtgcac gatggacgat ttcttgctcc agcgcgtcca gatactgatc ggccttgtgc
    40681 tgctggcgag taacaatctt attgcaaccg cagaagtaac aaagcttatg gcagaacggg
    40741 atatgtacgt agagagataa tggacgctca ggatagcgcg ccacggcttg taaaaacgcc
    40801 tgttcgccga agtcttctga aaactccagc gcggtcgggt acgaggtgta tcgtggcccg
    40861 gaatagttat atttctggat cagggccaga tcccagtcga tttgctgtac agacatgctc
    40921 actccttccg atggcgtctc tggcggctac ggcgtaccgg cggatgcccg tttcgggtca
    40981 tcagccggtt gcgcagccac ttttgtcgcc gcgacaaccg ctgtagttta acgaataacc
    41041 acaccagata acatataacc ggaagggtta tcagcagaac gggaaggccc acggcggcaa
    41101 tccgttagtt gccccgcagc agacgcatca tgtcttcttg cttctcgtct tcttcctctt
    41161 cttcgtcatc gtcataagag aggccgagtt tctgcatcaa ctcatcaata cgatccagtt
    41221 tagcatccac ccaggattgc tcttcggcgc tcagggtttc gcctgcttcc agacgttcca
    41281 gcagcgcatc cagacgctca tccgtttcca gtaattccaa ctccgcctgc ggtgaaagca
    41341 taggtttctc actcttcggt ttgtgctgtt tggtgacttt ttcaatcacg cccaatggaa
    41401 taggggtttt actgccaata cgtggatctt ttggtgcgtt ctggcctttg ctgcctgacg
    41461 tggtgtttcc gcccgctgcg cggctgcccg gcgcatgacc acgacgtttt ttctgacgct
    41521 tgcgatcacg agcttcctga tccagctcct cgcgtgtttt tcgacgcgct tttgcatgac
    41581 ctttgctgcg tgagtttgaa gatgatggtt tcataatggt tcgtctttta gccgttttat
    41641 tcagtataga tttgcggcgg aatctaacag aaagcaagca aagaaaaaag gcgacagatt
    41701 actctgtcgc cttttttcct gactcataac ccttaacggg tcttacaatc cttgcaggcc
    41761 gccaacacac cctgtttttc ctttagccgg aacaatccgt gttgattcca tttccgttta
    41821 attacgtctc cggacgtttg tcttcctgac aatcctctgt cttcgcctcc tggcgctcct
    41881 gaccctcatc ctgagtcatt gttcctgtta gcgtcctcgc tctatgggtg ctactttacg
    41941 cctttgcttt aaacaaacaa tcaaccgcca tcagtatttg cacattttca gagttttata
    42001 taaaagcaat tcactgattt atatagctta tttgacatgc atcgcagaat tttctctgct
    42061 atttcgcata aagactatcg gcaatgtctc acaaagcgta tggcttttac ttacaacgct
    42121 cacgccgaaa aatccgcctt ttattatggt tcaggtataa tcgccacaaa gctttgactg
    42181 acggagacga ccgctttgac taatttgaat tatcaacaga cgcattttgt gatgagtgcg
    42241 cctgatattc gccacctacc ttccgatacc ggaattgaag tggcttttgc aggccgttcc
    42301 aacgcaggta aatccagcgc gctgaacacg ctgactaacc agaaaagcct ggctcgtacc
    42361 tcaaaaaccc cagggcgcac ccagcttatc aacctgtttg aagtggctga cggcaagcgt
    42421 ctggttgact tgcctgggta cggttatgcg gaagtcccgg aagagatgaa gcgcaaatgg
    42481 cagcgtgcgc tcggcgaata cctcgaaaaa cgtcagagcc tgcaaggtct ggtggtgcta
    42541 atggatattc gccatccgct gaaagatttg gatcagcaga tgattgagtg ggcggtagac
    42601 agcaatatcg ccgttctggt gctgctgacc aaagcggaca aactggcaag cggcgcacgt
    42661 aaagcgcaat tgaatatggt gcgtgaagct gtactggcgt ttaacggtga tgtgcaggtt
    42721 gaaacgtttt cttcgttgaa gaaacaaggc gtggacaagc tgcggcagaa actggatacc
    42781 tggtttagcg agatgcagcc tgtagaagaa acgcaggacg gcgaataatt ttcttgcctt
    42841 aatgcttgtg ccggatgtgg cgtatccggc ccgtaaattc aatagattac aattctaccg
    42901 ttggcctgat aaacatagcg catcaggcat tacggatctt ttctttcgcc caataaaaaa
    42961 cgccccagtc attactgact ggggcggcta aaatattcag ccaaatccga ttacgtgaag
    43021 taaaaggtct gaaagataga acatcttacc tctgtaccct acgcgactaa ctttactctt
    43081 ttttgttcag ttcagaaagc actttttgta attttttttc agttgttgca taggaatttc
    43141 taatagccat cacaaaacgt gacagcttat gttgcttact tacgaaaaaa gggcatgttc
    43201 aggcgaatct tagtgcgcct gatcccagtt ttcgccactc cccacttcca ccagcaacgg
    43261 cacatccaga cgggtacagt tttccatcag ttgatgaatc tgcttcgcga cggcatcaac
    43321 atcatcttta tgaacttcaa ataccagttc atcgtgtacc tgcatgatca tacgtacacg
    43381 cggttgctca gcctgtaacc acgcatcaac ggcaatcatc gcccgtttga taatgtcggc
    43441 ggcggttccc tgcattggcg cgttaatggc tgcacgttca gccgctgcac gacgagcacc
    43501 attgctggat ttgatatccg gcagatacag acggcgtccg tccagcgttt caacgtagcc
    43561 ctgctctttc gcctgagcac gggtgcgttc catatactcc agcacgccag ggtagcgttc
    43621 gaagtaaagg tccatgtact tctgcgcttc tttacgtgga atgttcaatt gccgcgccag
    43681 accgaaagca ctcatgccat aaatcagacc aaagttgatc gctttcgcgc tacggcgttg
    43741 ctcgctggtg acggtttcca gtggcaaacc aaacacttct gccgccgttg cccggtggat
    43801 atcttttcct tccgcgaatg cggtcagcaa gcctttgtca cgcgagagat gcgccataat
    43861 gcgcagttca atctgcgagt agtccgctga gacaatcaca taatcctctg gcgcaataaa
    43921 cgcctggcgg atacgacgac cttcttcgtt acgcaccgga atgttttgca ggttaggatc
    43981 ggttgacgat aaacgtcccg ttgcggttac tgcctggtga taagaggtat gcacacgccc
    44041 ggttttcggg ttgatcatca gcggcagctt gtcggtgtaa gtcgacttca gcttcgccag
    44101 accacgatac tccagaatca cttttggcaa cggatagtcc agcgccagtt cttccagtac
    44161 ctcttccgac gttgacggcg cgccacccgg cgttttcttc agcggtttaa taccctgttt
    44221 ttcaaacaga atggtttgca actgcttggt ggaagaaagg ttaaattctt cacctgcaat
    44281 ttcatgcgct ttcttttcca gctcagccag acgcaaggtg agctcttcag aatgattgtg
    44341 cagcactttc ggatcgatct tcacaccgtt acgttcaatg cgtgaaagca ctggcaccag
    44401 cggcatttcg atattctcga agacgttcaa cggccctttg tgtttttgca gatccggcca
    44461 cattttcaga tgcaactgca aggtgacatc agcatcttcg gcggcgtaac gtccggcctc
    44521 ttcgagggca atctggttaa aggtcagttg atttttgcct ttacccgcaa tctcttcaaa
    44581 agtgatggtt ttgtgcttca accaacgttc cgcgaggctg tccatatcgt gacgcccggc
    44641 aacgctattg agaatgtagg actccagcat ggtatcaaac gcaatcccac gcagctcaat
    44701 gccgtaattc gccagaatac cgcgatcgta tttcaggttt tgcccgacct tcagcgcctt
    44761 ttcatcttcc agcagcggtt ttagcaactc gagtgcacgc tcgcgagaga tttgatcggg
    44821 cgcgtcaaga taatcatgag caaccggaat atatgccgct acgcctggct cgatagcaaa
    44881 agaaagcccg accaggttag cagagatgtt atcaaggctg tcggtttcgg tatcaaatgc
    44941 aaataccggc gctttttcca gcttcgcaat ccacgctttc agtgtttctt catcaaggat
    45001 ggtgacgtag ttgtcataag aaatcaccgt tgccgtcact tctggcgctt cgtctgcaac
    45061 actggtttcc tgcggcttcg cggctggttt tgcccctttg gcctgtaacc atttgcccgc
    45121 ttcgacatca gcagtccagc gtttgaactc atactttttg aacagcccca acaactcttc
    45181 cgctgccggt tgctgcactt ccagttgttc acaggtcagc tccagttcaa cgtcggtttt
    45241 aatcgtcgcc agctggtatg agagataagc aacttctttg ttttgctcga gcttcgctgc
    45301 cattgttttc gcgccacgga agctcaaccc agcaattttt tctggctcgg catacagcgt
    45361 atccagcccg ccaagacctt gcagcaatgc ctgcgcggtt ttttcaccga cgcccggtac
    45421 gccaggaatg ttatcggagg agtcacccat cagcgccagg aaatcgatga tcagttctgg
    45481 cggcacgccg tacttattca ccacctcttc cggtccgagg atggtattcg tcatggtatt
    45541 gataagcgta atatttggcg tcaccagctg cgccatatct ttatcgccag tgctgatcag
    45601 caccggacgc ccggcttttt cggcttcgcg cgccagagta ccgataacgt cgtccgcttc
    45661 tacgccagaa accgccagca gcggcagtcc catcgcttta accatcgcgt gcaggggttc
    45721 tatttgtgct cgcagatcgt tcggcattgg cgggcgatgt gatttgtaat gttcaaacag
    45781 ttcatcacga aaggtttttc ccttggcgtc aaagaccacc gctgcatgcg tcggtttata
    45841 ttgcatgatc agactgcgca gcatgttgag gacaccatac atcgcaccgg tcggctcgcc
    45901 tgcgctgtta gtcagcgggg gaaacgcgtg atatgcgcga taaagataag atgaaccatc
    45961 tacaaggata agtggatttt gggggatctg aaccataatg tccgtgcctg tttatcagat
    46021 tatgggtaaa ggatgccaca gacaggatga aaacatgagt ttttcaccgc ttttcgtgaa
    46081 aatcttttgt agatcttctg gatcgctcgc aaatcgtcat gtggataact ttgtgcataa
    46141 attatttaca tcgcttttaa tttattatca tgctttttac agcatgatgt ttgtctttgt
    46201 ttttcatgca gttaaaaata aagccatccc taataattga gataatggga ttttttctaa
    46261 tgtggataga tatgaattat ttttctcctt aaggatcatc cgttatttgg gtcgttttct
    46321 gctaaaatct gcgccttgcg cttctttagc gcactgtcta tggctaacct tttgaataaa
    46381 ttcattatga cgagaatact cgctgcgata acccttttgc tgagtatcgt attgaccatt
    46441 ttggtcacta ttttttgttc cgtcccgatc atcattgccg ggattgtaaa actattgcta
    46501 cctgtgccag tcatttggcg aaaggtttcg cgtttttgtg attttatgat gtattgctgg
    46561 tgtgaagatc tggcggtatt actgcatctg aacccacacc tgcaatggga agttcacggg
    46621 ctggaagggt taagtaagaa gaactggtat ctgcttattt gtaaccaccg tagttgggca
    46681 gatattgtcg tactgtgcgt gctgtttcgt aagcacattc cgatgaataa atatttcctc
    46741 aagcaacagc tggcctgggt gccatttctt ggcctggcgt gctgggcgct ggatatgccg
    46801 tttatgaagc gttattcccg cgcttattta ttacgtcatc cggagcgacg tggtaaagat
    46861 gttgaaacca ctcggcgttc ttgtgaaaag tttcgcctgc atcccaccac cattgtcaat
    46921 ttcgttgaag gctcccgttt cacgcaagaa aaacatcagc aaacccactc cacttttcaa
    46981 aacttgttgc caccaaaggc ggcaggcatt gcgatggcgc taaatgtact gggtaaacaa
    47041 ttcgataaac tgcttaatgt tacgctgtgt tatccggaca ataaccgtca gccgttcttc
    47101 gatatgttaa gcggtaaact gacgcgaatt gtcgttcatg tggatttaca gcctattgcc
    47161 gatgagttac atggcgacta catcaatgac aaaagcttta agcgccattt tcagcaatgg
    47221 ctgaactcac tgtggcagga aaaagaccga cttctgacgt cgttgatgtc atcacagcgg
    47281 caggacaagt aaattttgct cgcctctgaa tgagcagagg cgagtgagta ttttaatgaa
    47341 caaaacgtcc ggcacgagac ataaattctt cttcgctcat ctcctgtccg ttaaaaacaa
    47401 ctttacccgg tgtatcacgc agttgcagcg aggcggtatt gttgtcaatc gtgattaact
    47461 gaaacatctg acctaaggtc atcatcccgc tgatttgttt atcagcccgc ttttgcgctt
    47521 tttccgcatc cattccttca gataaattaa gctgttttgc ggtttctgtg gcgacattaa
    47581 gcggtaactt cacatcaaaa ttgagcgatt taatatcttt gtttgtggat gatgaagatt
    47641 tggctgggtc ggcaatactg atatccagat tggcatttag ttcgccgagt gcgttcttcc
    47701 attttaccgg ttgtttaatg gtcggctcac ttttttgtaa taacggtaaa tactctttga
    47761 acaatgcagc attcacttct tgcagagcaa cttcatcgtt tgctaactca gggtgtgcag
    47821 caagctgctt ctgcattgca atgttatact ggatgataaa ttggcgcacc gctgagggat
    47881 caatagattc agcaatcaat gaccactcac cactaccgag ttgctgattc ccttttgtta
    47941 acttatcaag ttcgtaggtc agattgacat tgatataatc tttgtcttta acgcgatcca
    48001 gcatggtttt tgcaacgaaa gcatcgattt gggcaacgtc ttccccgtga ttgataatgt
    48061 taatctgatt aaatttcgac tcgctttcac caaccggaaa tttcttttct tctaatcgcg
    48121 ccagagaatc aatggtaaaa ctttttgccg ttactttgtt cattggtgat aattgcagaa
    48181 caatatcttc gactttgcct tcaatatcta ggttagacaa tgaactacca tcaccattaa
    48241 aagtaaactg gccctcacca aaagaaaact ttccatactc tgcaggggca acatctaatg
    48301 tcgtagagaa tgagccacca aacgcgaatc gcaatgtcgc ttccacgggc gatttatttt
    48361 tcgcggcgat aaatagcggt tgtgtcagtt cattattcac taagttcact tttgccgctg
    48421 ccagtgctgg gatgacatta ccatgcatta acattgtgat gggcaacgga ccgtgctcaa
    48481 catccacatc aaatacaact ttctggcctg gcttgatatt gagatcgggt gcaccgttat
    48541 cgaatgtcat ttgcatttga aaacgagaat taaaaaaacc tttgtcaaaa tttttataac
    48601 ttaacgtcat atcgtaggca tgttcacctt tctttttagc atcgttaaaa tctttaataa
    48661 acttttcgac accaggctga atttgcgtac ctgtgtacca tgtgccacca ccccagatta
    48721 cggctaacgc aacaataaca cctgtagctg acttatgtat catataaata ttccctgata
    48781 aaacagcaaa actttaaata tccgcttatg ctgaaataat gtccagc
//
LOCUS       DAJOWB010000021        48438 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJOWB010000021 DAJOWB010000000
VERSION     DAJOWB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.021"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            321..1538
                     /gene="alaA"
                     /locus_tag="OHO85_001366"
     CDS             321..1538
                     /gene="alaA"
                     /locus_tag="OHO85_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCQ2670767.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1622..2221
                     /gene="yfbR"
                     /locus_tag="OHO85_001367"
     CDS             1622..2221
                     /gene="yfbR"
                     /locus_tag="OHO85_001367"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCQ2670768.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     ATIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2280..4112)
                     /locus_tag="OHO85_001368"
     CDS             complement(2280..4112)
                     /locus_tag="OHO85_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCQ2670769.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4199..4849)
                     /gene="hxpA"
                     /locus_tag="OHO85_001369"
     CDS             complement(4199..4849)
                     /gene="hxpA"
                     /locus_tag="OHO85_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCQ2670770.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLSHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4860..5354)
                     /locus_tag="OHO85_001370"
     CDS             complement(4860..5354)
                     /locus_tag="OHO85_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCQ2670771.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5437..5892)
                     /locus_tag="OHO85_001371"
     CDS             complement(5437..5892)
                     /locus_tag="OHO85_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCQ2670772.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6230..7432
                     /gene="ackA"
                     /locus_tag="OHO85_001372"
     CDS             6230..7432
                     /gene="ackA"
                     /locus_tag="OHO85_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCQ2670773.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7507..9651
                     /gene="pta"
                     /locus_tag="OHO85_001373"
     CDS             7507..9651
                     /gene="pta"
                     /locus_tag="OHO85_001373"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCQ2670774.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9841..11361
                     /gene="yfcC"
                     /locus_tag="OHO85_001374"
     CDS             9841..11361
                     /gene="yfcC"
                     /locus_tag="OHO85_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCQ2670775.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11394..11936)
                     /gene="yfcD"
                     /locus_tag="OHO85_001375"
     CDS             complement(11394..11936)
                     /gene="yfcD"
                     /locus_tag="OHO85_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCQ2670776.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11994..12545)
                     /gene="yfcE"
                     /locus_tag="OHO85_001376"
     CDS             complement(11994..12545)
                     /gene="yfcE"
                     /locus_tag="OHO85_001376"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCQ2670777.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12601..13245)
                     /gene="yfcF"
                     /locus_tag="OHO85_001377"
     CDS             complement(12601..13245)
                     /gene="yfcF"
                     /locus_tag="OHO85_001377"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCQ2670778.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13381..14028
                     /gene="yfcG"
                     /locus_tag="OHO85_001378"
     CDS             13381..14028
                     /gene="yfcG"
                     /locus_tag="OHO85_001378"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCQ2670779.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14085..14447
                     /gene="folX"
                     /locus_tag="OHO85_001379"
     CDS             14085..14447
                     /gene="folX"
                     /locus_tag="OHO85_001379"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCQ2670780.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14468..15361
                     /locus_tag="OHO85_001380"
     CDS             14468..15361
                     /locus_tag="OHO85_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCQ2670781.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15409..16299)
                     /gene="rpnB"
                     /locus_tag="OHO85_001381"
     CDS             complement(15409..16299)
                     /gene="rpnB"
                     /locus_tag="OHO85_001381"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCQ2670782.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16496..17269)
                     /gene="hisP"
                     /locus_tag="OHO85_001382"
     CDS             complement(16496..17269)
                     /gene="hisP"
                     /locus_tag="OHO85_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCQ2670783.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVSGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17277..17993)
                     /locus_tag="OHO85_001383"
     CDS             complement(17277..17993)
                     /locus_tag="OHO85_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2670784.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17990..18676)
                     /gene="hisQ"
                     /locus_tag="OHO85_001384"
     CDS             complement(17990..18676)
                     /gene="hisQ"
                     /locus_tag="OHO85_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCQ2670785.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18766..19548)
                     /gene="hisJ"
                     /locus_tag="OHO85_001385"
     CDS             complement(18766..19548)
                     /gene="hisJ"
                     /locus_tag="OHO85_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCQ2670786.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19736..20551)
                     /gene="argT"
                     /locus_tag="OHO85_001386"
     CDS             complement(19736..20551)
                     /gene="argT"
                     /locus_tag="OHO85_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCQ2670787.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGDWYVAGKLYLME"
     gene            complement(20817..21386)
                     /gene="ubiX"
                     /locus_tag="OHO85_001387"
     CDS             complement(20817..21386)
                     /gene="ubiX"
                     /locus_tag="OHO85_001387"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCQ2670788.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21481..22998)
                     /gene="purF"
                     /locus_tag="OHO85_001388"
     CDS             complement(21481..22998)
                     /gene="purF"
                     /locus_tag="OHO85_001388"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCQ2670789.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23035..23523)
                     /gene="cvpA"
                     /locus_tag="OHO85_001389"
     CDS             complement(23035..23523)
                     /gene="cvpA"
                     /locus_tag="OHO85_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCQ2670790.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23782..24444)
                     /gene="dedD"
                     /locus_tag="OHO85_001390"
     CDS             complement(23782..24444)
                     /gene="dedD"
                     /locus_tag="OHO85_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCQ2670791.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24434..25702)
                     /gene="folC"
                     /locus_tag="OHO85_001391"
     CDS             complement(24434..25702)
                     /gene="folC"
                     /locus_tag="OHO85_001391"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCQ2670792.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25772..26686)
                     /gene="accD"
                     /locus_tag="OHO85_001392"
     CDS             complement(25772..26686)
                     /gene="accD"
                     /locus_tag="OHO85_001392"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCQ2670793.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26842..27501)
                     /locus_tag="OHO85_001393"
     CDS             complement(26842..27501)
                     /locus_tag="OHO85_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCQ2670794.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27584..28396)
                     /gene="truA"
                     /locus_tag="OHO85_001394"
     CDS             complement(27584..28396)
                     /gene="truA"
                     /locus_tag="OHO85_001394"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCQ2670795.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     LVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28396..29409)
                     /locus_tag="OHO85_001395"
     CDS             complement(28396..29409)
                     /locus_tag="OHO85_001395"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCQ2670796.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEACDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29475..30611)
                     /gene="pdxB"
                     /locus_tag="OHO85_001396"
     CDS             complement(29475..30611)
                     /gene="pdxB"
                     /locus_tag="OHO85_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCQ2670797.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30710..31705
                     /gene="flk"
                     /locus_tag="OHO85_001397"
     CDS             30710..31705
                     /gene="flk"
                     /locus_tag="OHO85_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCQ2670798.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31702..32880)
                     /locus_tag="OHO85_001398"
     CDS             complement(31702..32880)
                     /locus_tag="OHO85_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HCQ2670799.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33164..34384)
                     /gene="fabB"
                     /locus_tag="OHO85_001399"
     CDS             complement(33164..34384)
                     /gene="fabB"
                     /locus_tag="OHO85_001399"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCQ2670800.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34543..36549
                     /gene="mnmC"
                     /locus_tag="OHO85_001400"
     CDS             34543..36549
                     /gene="mnmC"
                     /locus_tag="OHO85_001400"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCQ2670801.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTIPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36670..36948)
                     /locus_tag="OHO85_001401"
     CDS             complement(36670..36948)
                     /locus_tag="OHO85_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCQ2670802.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(36982..37530)
                     /gene="epmC"
                     /locus_tag="OHO85_001402"
     CDS             complement(36982..37530)
                     /gene="epmC"
                     /locus_tag="OHO85_001402"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCQ2670803.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37530..38339)
                     /locus_tag="OHO85_001403"
     CDS             complement(37530..38339)
                     /locus_tag="OHO85_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCQ2670804.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(38339..39163)
                     /gene="mepA"
                     /locus_tag="OHO85_001404"
     CDS             complement(38339..39163)
                     /gene="mepA"
                     /locus_tag="OHO85_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCQ2670805.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39167..40252)
                     /gene="aroC"
                     /locus_tag="OHO85_001405"
     CDS             complement(39167..40252)
                     /gene="aroC"
                     /locus_tag="OHO85_001405"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCQ2670806.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40287..41219)
                     /gene="prmB"
                     /locus_tag="OHO85_001406"
     CDS             complement(40287..41219)
                     /gene="prmB"
                     /locus_tag="OHO85_001406"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCQ2670807.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41385..41936
                     /gene="smrB"
                     /locus_tag="OHO85_001407"
     CDS             41385..41936
                     /gene="smrB"
                     /locus_tag="OHO85_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCQ2670808.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42058..42930)
                     /locus_tag="OHO85_001408"
     CDS             complement(42058..42930)
                     /locus_tag="OHO85_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCQ2670809.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(42917..43441)
                     /locus_tag="OHO85_001409"
     CDS             complement(42917..43441)
                     /locus_tag="OHO85_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2670810.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(43438..43908)
                     /locus_tag="OHO85_001410"
     CDS             complement(43438..43908)
                     /locus_tag="OHO85_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2670811.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(43905..44453)
                     /locus_tag="OHO85_001411"
     CDS             complement(43905..44453)
                     /locus_tag="OHO85_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2670812.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(44428..45180)
                     /locus_tag="OHO85_001412"
     CDS             complement(44428..45180)
                     /locus_tag="OHO85_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCQ2670813.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTPYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            complement(45200..47845)
                     /gene="yfcU"
                     /locus_tag="OHO85_001413"
     CDS             complement(45200..47845)
                     /gene="yfcU"
                     /locus_tag="OHO85_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCQ2670814.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEEFSGDDTQKKWEWSRYYAWRALPSLKAKLALGEDYLNSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNMSVSLTGY
                     RYEYDNRADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDD
                     IKPVIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(47927..>48438)
                     /locus_tag="OHO85_001414"
     CDS             complement(47927..>48438)
                     /locus_tag="OHO85_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2670815.1"
                     /translation="TSTATIAAGNNGTARFYGTIEDSVCSIVPDDHKLEVDMGDIGAE
                     KLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSANSGNYYTIFNTDTGAAFNN
                     VSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNFKAWLVGAADAPDLGNFEA
                     NTTFQITYL"
BASE COUNT        11985 a        13041 c        11990 g        11422 t
ORIGIN      
        1 aaactctccg taatgcggaa atatcatcat cgcgtttcat tagtgaattc ttaaatgagg
       61 cattttcaca caattatctt acagataaaa aaaccagact tacaattaag aatcagaaca
      121 attcactaca taacattgca tgtaaagcat atacacctca ttattttgtc attattaagt
      181 tattaacagc acaatcgagc cttcccctct ggcaaaatct tattctgcag accttcaaaa
      241 caccgtcctg ggggagtaca ttgttctaag ctgacttcca cggcagggag tggcgataac
      301 agcaaaaaag gtcaagattc atgtccccca ttgaaaaatc cagcaaatta gagaatgtct
      361 gttatgacat ccgtggtccg gtgctgaaag aagcaaaacg cctggaagaa gaaggtaaca
      421 aggtactgaa actgaacatc ggcaacccag ccccgttcgg ttttgacgcg ccagatgaaa
      481 tcctcgttga cgtgatacgc aacctgccta ccgctcaagg gtattgcgat tccaaaggtc
      541 tttactccgc gcgtaaagcc atcatgcagc actaccaggc tcgtggcatg cgtgatgtta
      601 ccgtggaaga tatttacatc ggcaatggtg tatcggagct tatcgttcag gcaatgcagg
      661 cattgctgaa cagcggggac gaaatgttgg ttcctgcacc agattaccca ctatggaccg
      721 cggcggtttc gctttccagc ggtaaagcgg tgcattatct ttgcgatgaa tcctctgact
      781 ggttcccgga cctcgatgat attcgcgcta aaattacgcc tcgtacgcgt gggatcgtta
      841 ttatcaaccc aaataaccca accggcgcgg tatattccaa agagctttta atggagattg
      901 tggagattgc acgtcagcat aatctcatta tcttcgccga tgaaatttat gacaaaattc
      961 tttacgacga cgctgagcat cactcaattg cgccgctggc acctgacctg ctgaccatta
     1021 cctttaacgg actgtcgaaa acgtaccgcg ttgcaggctt ccgtcagggg tggatggtgt
     1081 tgaacgggcc gaaaaaacac gccaaaggct acatcgaagg tctggaaatg ctggcttcaa
     1141 tgcgcctgtg tgctaacgtt cctgcgcaac acgccattca gaccgcgctg ggtggttatc
     1201 agagcatcag tgaatttatt acccctggcg gtcgtcttta tgagcagcgt aaccgcgcgt
     1261 gggaactgat caacgatatt ccgggcgttt cctgcgtgaa acctcgtggt gcgctgtata
     1321 tgttcccgaa aatcgacgcc aaacgcttta acattcacga cgatcagaaa atggtgctgg
     1381 atttcctgtt gcaggaaaaa gttctgttgg tgcaagggac ggcattcaac tggccgtggc
     1441 cggatcactt ccgcattgtc acgctaccgc gtgtcgatga tatcgagctg tctttgagca
     1501 agttcgcgcg tttcctttct ggttatcatc agctgtaatc ttaatttcac tgccggagat
     1561 tgcatccggc agcgttatcc cgccacaatg acctgatgat gtcatcatac gtaaggtcac
     1621 tatgaaacag agccattttt ttgcccatct ctcccgcctg aaactcatta accgctggcc
     1681 gctcatgcgc aacgtgcgga cggaaaatgt gtccgaacac agtttgcagg tagcgatggt
     1741 cgcccatgcg ctggcaacta tcaaaaatcg gaaatttggc ggtaatgtca acgccgaacg
     1801 tatcgcttta ctggcgatgt accacgatgc ctcagaagtg ctcaccggcg atctccctac
     1861 tccggtgaaa tacttcaatt cgcaaatcgc tcaggagtac aaggctattg aaaaaatcgc
     1921 tcagcaaaaa ctggtcgata tggttccgga agagctgcgg gatatctttg cgccgttaat
     1981 tgacgagcat gcatatagcg atgaagaaaa atcgctggtg aaacaggcag atgcactgtg
     2041 tgcatatctg aaatgtctgg aagaactcgc ggccggaaat aatgaattct tgctggcaaa
     2101 aacgcgactg gaagcgacgc ttgaagcgcg tcgcagccag gagatggact acttcatgga
     2161 agtatttgtt cccagcttcc atctttcgct cgatgagatt agccaggatt caccgctgta
     2221 agcagccgga gtctgcgtcg catcaggcaa taagcgccgg atgcgacatc aggctcttgt
     2281 caaaacggaa acagcatcgg gatcatcacc acacaaaccg ccatcacgat aatggtgaac
     2341 ggtaccccca acttcacaaa gtcactgaag ctgtaatttc ccggacctaa aaccagtgtg
     2401 ttaacaggtg aagaaaccgg tgtcataaag gcggcggatg ctgccatcgc cacgaccatc
     2461 gcgaatggat aaggcgacac ccccatcgtt ttggcagcag ccagcgcaat cggagccatc
     2521 aacaccgccg tcgcggtatt agagataaat agcccaataa ccgccgacaa gacaaacaaa
     2581 caacccagca tcatatgtgg cccgtaacca ccgccaatgt ccatcagccc tttcaccgcc
     2641 agcgcgacac ctcccgtttt ctgtaatgcc acagcaaacg gcatcatccc aacgatcaaa
     2701 ataatgctcg gccagtgaat ggatttatag gcgctttcag catctataca gcggaatttc
     2761 cccatcagca ggcaggcgat gatagcggca acaggattag gaatttcatc tgtcagcatt
     2821 aacgccacca ttagcaccag acagaaaatg gcatggggtg cctggctgtg cgcgggcgat
     2881 gcttcactca cctcttccgg taagttcagc gctacgaagt cgcggccctg tttggccagc
     2941 ataccgatca gtttccagtt acccacaacc aggatgatat cgcccagcag cagaggctca
     3001 tccgccagcg aaccttccag cgccacgcca ttgcgcttta gccccaccac attcagtccg
     3061 tagcgggtac gaaaaccaat ttcgcgcacc gatttaccaa tcagttctga ctcaggaatt
     3121 aatgaaatct ctgccatgcc cacatcaagg gcctggtcag aaaaatactc gccgcgcagt
     3181 accatcggct ccagcaattg ctcactacaa aattgccgga gatcgacatc agccgcagac
     3241 atatcaataa gcaaaacgtc acgcgcgcga aattcagaaa ccccattaac gttcacgata
     3301 acgcgacgaa aacgccgcca gcgttcaaca ccgatgacgt tagcgccata acgctcacgt
     3361 aatttgagat catccagccg ttgacctatc attggcgatc cggggcgaat agccagacgt
     3421 cgcgcacgcc cggtcagtcg atattcacgg ataagatcgc gaaaggttcg acgcgtccag
     3481 ccttcgcgct gcggggtctg ggtatcccct ttcagcatga aacgcatcac taacatatac
     3541 aaaataccca gcaccagcac gaccaggcca ataggtgtta cgctaaagaa actgaagccg
     3601 tgatagcctt cacgcagcaa ttcactgttg actaccaggt tcggcggcgt cgccaccagc
     3661 gtcatcatgc cgcttatcag cccggcaaaa ctcaacggca tcatcagacg tgacggcgaa
     3721 gtttgcatac gcatggcaac gcttaacacc acgggaataa agatagcgac aacgccggtt
     3781 gaactcataa acgcgccaag ccccgcgacg gtcagcatca acaaaaccaa cattttgatt
     3841 tcactgttgc ccgcaacttt gaccagccat gttcccatta cggtggcaac accggtacgg
     3901 accaaaccat cgccaataat aaacaaggcg gcaatcagga caacgttagg atcagaaaag
     3961 ccggaaaata cttcggggac tgtcagcgtt ccgcttaatg caaacgcgac aataacaaac
     4021 aaagcgaccg catccatacg cactctgccc gtcgcaaaca agacaatggc aacggccagt
     4081 aatgaaagaa cccaaatcaa ttcaccgttc acaacttatc cttgttaatt gagggagatg
     4141 tcttgattct gccataaaaa agccccgacg agacggggct aaatcatgat caggtgtttc
     4201 actgaataat aacatcgcca tttggctgtt tggtcacagt aatttgctcc agactgtgga
     4261 ggaccaaatc gacctcattc aggcgcgggg tatctgccgg agcgttaacc gcaatgacat
     4321 ggcaacccgc cgccaggcca gaaagcacgc cagcgggagc atcttccacc accacacact
     4381 cctgcggcgc aagccccagc agctgcgcac ctaacagata cgcatcaggt tctggttttc
     4441 cgcgcttcac tcgctcagcg gttacaaaca cctctggtgc gggaagccca gctattttat
     4501 ggcgcgctcg cgctaccggc atggagccag aagtcacaat ggcccacgga atacctgctt
     4561 tattcaaatg actgagtaag gcgattgccc ccggaagcgc ggtaatacct tcggtttccg
     4621 tggcctcgat ttgctccaga cgcgtaaact cggcggcaat atcagcctcg gatttgcccg
     4681 ccataaaatg gcgcagagag gtgatcgcct gtttaccgtg aatgaaagcc agcacctctt
     4741 ccggcgctaa cccatgacgt ctggcccagt tgctccacgc ccgttctacc gcaggcaggg
     4801 aatccaccag cgttccatca agatcaaaca gaaaaccttt gcaccgcacg cgggcctcct
     4861 caggcgttga taatttgatt aatttcgttg gcgctcaaat ggtactgacg cgggcaggca
     4921 tgccacacat taagcatgcg ctgatatttt tcccacattg gcgtctgggc gttaaaaccg
     4981 tgagttccgg cgtcaaaatg ggtatagcgc ccttccacat taaccataaa gcggacataa
     5041 ccgaggtaac gtgcttcagt ggcggcgtca aagccgagga aggtgacacg acgttcatcg
     5101 atggattgct ggtcctgcaa attagaccag gaaacatgca acgcatgata catctccata
     5161 atgtcgatga tagtgcggca ggtttcttct ttcagctcgc caaactcgcg atccaattca
     5221 cgcatctgta atccgtaacc acgctcaata attgtttgca ggcgacggta acgttcagca
     5281 tttgccggat cgagcatagt catcatcttg tactgattag acaaaataag acgttgcgcg
     5341 ttggtcattt ccattgttga ctcctgtatc actatactac ggtgaaaaaa aagaaggctg
     5401 agtatgcctt cttttatatg cgtaatcagg ggtcaattac aaatcatcaa ggaaagtttt
     5461 atccagttgt ttgaaggcgc gcttaagcgt gtcagctaat gcctggtaat caggcttgcc
     5521 ttcaacgggt gccaacacct gtccagactc ctgcaattta ccgcgaactt cataaaacca
     5581 gttaaggatt gcagggggta atggcgttac agaacgcttg cccagccacc acaatccctg
     5641 catgggtaaa cttaaggcga acagcgcagt ggcaactgcc ggcccaagct gaccgcccag
     5701 ggcaatctgc cagcagagag taaatacggc gatcggcggc ataaaacgga tcgcataacg
     5761 cgtcatcttg ataacgcgat tttcgacaaa gaccggggca aggcgttttt ccagtggcca
     5821 cgtctttgag taatgctgtc cgcggcgaaa caagctaaaa aaattaacag aacgattatc
     5881 cggcgttgac atgcttcacc tcaacttcac atataaagat tcaaaaattt gtgcaaattc
     5941 acaactcagc gggacaacgt tcaaaacatt ttgtcttcca tacccactat caggtatcct
     6001 ttagcagcct gaaggcctaa gtagtacata ttcattgagt cgtcaaattc atatacatta
     6061 tgccattggc tgaaaattac gcaaaatggc atagactcaa gatatttctt ccatcatgca
     6121 aaaaaaattt gcagtgcatg atgttaatca taaatgtcgg tgtcatcatg cgctacgctc
     6181 tatggctcac tgacgttttt ttagccacgt atcaattata ggtacttcca tgtcgagtaa
     6241 gttagtactg gttctgaact gcggtagttc ttcactgaaa tttgccatca tcgatgcagt
     6301 aaatggtgaa gagtaccttt ctggtttagc cgaatgtttc cacctgcccg aagcacgtat
     6361 caaatggaaa atggacggta ataaacagga agcggcttta ggtgcaggcg ccgctcacag
     6421 cgaagcgctc aactttatcg ttaatactat tctggcacaa aaaccagaac tgtctgcgca
     6481 gctgactgct atcggtcacc gtatcgtaca cggcggcgaa aagtatacca gctccgtagt
     6541 gatcgatgag tctgttattc agggtatcaa agatgcagct tcttttgcac cgctgcacaa
     6601 cccggctcac ctgatcggta tcgaagaagc tctgaaatct ttcccacagc tgaaagacaa
     6661 aaacgttgct gtatttgaca ccgcgttcca ccagactatg ccggaagagt cttacctcta
     6721 cgccctgcct tacaacctgt acaaagagca cggcatccgt cgttacggcg ctcacggcac
     6781 cagccacttc tatgtaaccc aggaagcggc aaaaatgctg aacaaaccgg tagaagaact
     6841 gaacatcatc acctgccacc tgggcaacgg tggttccgtt tctgctatcc gcaacggtaa
     6901 atgcgttgac acctctatgg gcctgacccc gctggaaggt ctggtcatgg gtacccgttc
     6961 tggtgatatc gatccggcga tcatcttcca cctgcacgac accctgggca tgagcgttga
     7021 cgcaatcaac aaactgctga ccaaagagtc tggcctgctg ggtctgaccg aagtgaccag
     7081 cgactgccgc tatgttgaag acaactacgc gacgaaagaa gacgcgaagc gcgcaatgga
     7141 cgtttactgc caccgcctgg cgaaatacat cggtgcctac actgcgctga tggatggtcg
     7201 tctggacgct gttgtattca ctggtggtat cggtgaaaat gccgcgatgg ttcgtgaact
     7261 gtctctgggc aaactgggcg tgctgggctt tgaagttgat catgaacgca acctggctgc
     7321 acgtttcggc aaatctggtt tcatcaacaa agaaggtacc cgtcctgcgg tggttatccc
     7381 aaccaacgaa gaactggtta tcgcgcaaga cgcgagccgc ctgactgcct gatttcacac
     7441 cgccagctca gctggcggtg ctgttttgta acccgccaaa tcggcggtaa cgaaagagga
     7501 taaaccgtgt cccgtattat tatgctgatc cctaccggaa ccagcgtcgg tctgaccagc
     7561 gtcagccttg gcgtgatccg tgcaatggaa cgcaaaggcg ttcgtctgag cgttttcaaa
     7621 cctatcgctc agccgcgtac cggtggcgat gcgcccgatc agactacgac tatcgtgcgt
     7681 gcgaactctt ccaccacgac ggccgctgaa ccgctgaaaa tgagctacgt tgaaggtctg
     7741 ctttccagca atcagaaaga tgtgctgatg gaagagatcg tcgcaaacta ccacgctaac
     7801 accaaagacg ctgaagtcgt tctggttgaa ggtctggtcc cgacacgtaa gcaccagttt
     7861 gcccagtctc tgaactacga aatcgctaaa acgctgaatg cggaaatcgt cttcgttatg
     7921 tctcagggca ctgacacccc ggaacagctg aaagagcgta tcgaactgac ccgcaacagc
     7981 ttcggcggtg ccaaaaacac caacatcacc ggcgttatcg ttaacaaact gaacgcaccg
     8041 gttgatgaac agggtcgtac tcgcccggat ctgtccgaga ttttcgacga ctcttccaaa
     8101 gctaaagtaa acaatgttga tccggcgaag ctgcaagaat ccagcccgct gccggttctc
     8161 ggcgctgtgc cgtggagctt tgacctgatc gcgactcgtg cgatcgatat ggctcgccac
     8221 ctgaatgcga ccatcatcaa cgaaggcgac atcaatactc gccgcgttaa atccgtcact
     8281 ttctgcgcac gcagcattcc gcacatgctg gagcacttcc gtgccggttc tctgctggtg
     8341 acttccgcag accgtcctga cgtgctggtg gccgcttgcc tggcagccat gaacggcgta
     8401 gaaatcggtg ccctgctgct gactggcggc tacgaaatgg acgcgcgcat ttctaaactg
     8461 tgcgaacgtg ctttcgctac cggcctgccg gtatttatgg tgaacaccaa cacctggcag
     8521 acctctctga gcctgcagag cttcaacctg gaagttccgg ttgacgatca cgaacgtatc
     8581 gagaaagttc aggaatacgt tgctaactac atcaacgctg actggatcga atctctgact
     8641 gccacttctg agcgcagccg tcgtctgtct ccgcctgcgt tccgttatca gctgactgaa
     8701 cttgcgcgca aagcgggcaa acgtatcgta ctgccggaag gtgacgaacc gcgtaccgtt
     8761 aaagcagccg ctatctgtgc tgaacgtggt atcgcaactt gcgtactgct gggtaatccg
     8821 gcagagatca accgtgttgc agcgtctcag ggtgtagaac tgggtgcagg gattgaaatc
     8881 gttgatccag aagtggttcg cgaaagctat gttggtcgtc tggtcgaact gcgtaagaac
     8941 aaaggcatga ccgaaaccgt tgcccgcgaa cagctggaag acaacgtggt gctcggtacg
     9001 ctgatgctgg aacaggatga agttgatggt ctggtttccg gtgctgttca cactaccgca
     9061 aacaccatcc gtccgccgct gcagctgatc aaaactgcac cgggcagctc cctggtatct
     9121 tccgtgttct tcatgctgct gccggaacag gtttacgttt acggtgactg tgcgatcaac
     9181 ccggatccga ccgctgaaca gctggcagaa atcgcgattc agtccgctga ttccgctgcg
     9241 gccttcggta tcgaaccgcg cgttgctatg ctctcctact ccaccggtac ttctggtgca
     9301 ggtagcgacg tagaaaaagt tcgcgaagca actcgtctgg cgcaggaaaa acgtcctgac
     9361 ctgatgatcg acggtccgct gcagtacgac gctgcggtaa tggctgacgt tgcgaaatcc
     9421 aaagcgccga actctccggt tgcaggtcgc gctaccgtgt tcatcttccc ggatctgaac
     9481 accggtaaca ccacctacaa agcggtacag cgttctgccg acctgatctc catcgggccg
     9541 atgctgcagg gtatgcgcaa gccggttaac gacctgtccc gtggcgcact ggttgacgat
     9601 atcgtctaca ccatcgcgct gactgcgatt cagtctgcac agcagcagta atctcgtcat
     9661 catccgcagc tttgcgctgc ggatatctga accggaaata atcactattt ccggtttttt
     9721 attctcttaa tctgcattaa tcctttctga ttatcttgct taactgcgct gcatcaatga
     9781 attgcgccat ttcactttgc atacttacca ctttgttttg tgcaagggaa tatttgcgct
     9841 atgtccgcaa tcactgaatc caaaccaaca agaagatggg caatgcccga tacgttggtg
     9901 attatctttt ttgttgctat tttaaccagc cttgccacct gggtagttcc ggtggggatg
     9961 tttgacagtc aggaagtgca gtatcaggtt gatggtcaaa caaaaacacg caaagtcgta
    10021 gatccacact catttcgcat tctgactaac gaagcaggcg aacctgagta tcaccgcgta
    10081 cagctgttca cgacgggcga tgaacgcccg ggcctgatga acttcccgtt tgaaggatta
    10141 acctcaggat cgaaatacgg gacagctgtt ggcatcatca tgtttatgct ggtgattggc
    10201 ggcgcgtttg gcattgtgat gcgtacagga accattgata acggtatcct ggcgcttatt
    10261 cgccataccc gcgggaatga aattctcttt attcctgcgc tgtttattct gttttcactt
    10321 ggcggcgcgg tatttggtat gggagaagag gccgtcgcct ttgccattat catcgcaccg
    10381 ctaatggtcc ggctgggcta tgacagtatt accaccgtcc tggtgaccta tattgccacg
    10441 caaatcggtt ttgccagttc gtggatgaac ccgttttgtg tggtcgttgc tcaggggatt
    10501 gccggcgttc cggtgctttc tggctccggg ttgcgcatcg tggtgtgggt tatcgccact
    10561 ctgattggcc tgatctttac catggtgtac gcctcacgag tgaaaaagaa tcctcttctg
    10621 tcacgcgtgc atgagtccga ccgcttcttt cgtgaaaagc aggctgatgt tgaacaacgt
    10681 ccgtttacct ttggtgactg gctggtattg attgtcctga ccgccgtaat ggtctgggtg
    10741 atttggggcg tgatcgttaa tgcctggttt attccagaaa ttgccagcca gttcttcacc
    10801 atgggtctgg tgattggcat catcggcgtt gttttccgcc ttaacggcat gacggttaat
    10861 accatggctt catcctttac cgaaggggcg cgaatgatga tcgcccctgc cctgctggtg
    10921 ggtttcgcca aagggatttt gctgctggtc ggtaatggtg aagcaggtga tgccagcgtg
    10981 ttaaatacca tcctcaacag cattgccaat gccattagcg gtctggacaa cgctgtcgcg
    11041 gcctggttta tgttgctctt ccaggcggta tttaatttct ttgtgacatc cggttctggt
    11101 caggcggcgt taaccatgcc gttactggca ccgcttggcg atctggtcgg tgttaaccgt
    11161 caggttaccg tgctggcatt ccagtttggt gatggcttca gccacatcat ttacccaacc
    11221 tcagcttcgt taatggcgac gctcggtgtt tgccgggtgg acttccgtaa ctggctgaag
    11281 gtgggcgcga cactgcttgg actgctgttt attatgtcca gcgtcgtggt gatcggcgct
    11341 cagttgatgg gctaccacta aaaatgttaa gagccgcaat tgcggctctt ttttcattct
    11401 gccgtttcag tctctacggc ttcatttttg gcattgcgct tcatccacaa cgccagcgct
    11461 ttcagcgagt ctggagtgaa ctcatcgcag cgtgcggtga tttcttccgg cgtcagccag
    11521 caaacttcac tgacttcatc ttcctgtagg gcgaagggac cgtgagagac gcagctgaac
    11581 aatgcgcccc agacacggca atttttatct tcgaaataga actgcccgtg ctcagcaaag
    11641 gggacaccgg caatgcccaa ctcttcttcc gcttcgcgac gcgcggattc cagcagttgc
    11701 tcatcggcct ggaccacacc gcctgcggtc gcatctaaca tgccgggtaa aaagtctttt
    11761 gtctcggtac gacgctggac cagaattttg cccatgccat catgcacgac gatgtaagtt
    11821 gcacgatgac gcagacactg tgcccgcatt tgttcccggc tggcttgtgc aatgacttcg
    11881 ttctcttcat tgacaatatc cacccattca gtacttgcca aacgacgctg ttccattatc
    11941 aggaaacctt ctttttctgg cgctcttacg gcgcattttg agttgtgggt aaattacgga
    12001 ttaatcgcga cctgcgcaat gatactttga tcattgagtg cgattacact aagtacgtca
    12061 ttatccagca tgccataact cgccggatta ccgcctttcg gaatactcac cgagccgggg
    12121 ttgaagtgga aaatctcccc ccgctgttct gccactggca gatgggtatg accgtacacc
    12181 agcacatcgt tctggtttaa agcaggtaga ttttccgggc caaaaagatg accatgcgtc
    12241 aaaaacagac gttgtttttc cagtaatacc tgttgccacg gcgcggttat cgggaaatgc
    12301 agcagcattt gatccacttc gctgtcgcag ttgccgcgca cagcgataac cttatgtgcc
    12361 acttcattaa gccgttcagc gactttggct ggcgcgtaac cctccggtaa agcattacgc
    12421 gggccgtgat tcaacacgtc gccaaggatc accagccact gggcaccgct ttgggcaaac
    12481 aactccagaa cacgttccgt cgccggtaac gacccatgaa tgtccgatgc aaacatcagt
    12541 ttcatcactc actcctcgtc gaaaaaacgt aagctatgat actggattct gtcgttgcta
    12601 tcagccagat tgcttcgccg aaagtgcaat aaaacgctgg acagacgctc gctgccactg
    12661 gaatgtcgca taatccacca ggcgttctgg cacctcatcg ccatgtagta ccaggcggtt
    12721 aatcattagc gccagatcag tatcagcaat gcaccattca ccaaataaat tcggctgacc
    12781 cagtgctaac aaatgttctg ccatcgcgaa cagtttctct gcactggctt ttccctcggc
    12841 cgttagtggc gcttttttcg cccccgcaaa gacaacatcc gtcggacgct cttcgcggat
    12901 gggcatcaga tcgctgcgca gccaggcctg aatctgtcgt gcacgcgcac gattttctaa
    12961 atcaagcgga taaatacgtt cccaggtcgg tggcgcaaat cgatcttcca gatactccgc
    13021 aatggcagaa gattcactca actcaaaatc atcgatttgt aataacggca cacggcgtgt
    13081 ctgaccgtaa ccttgccacg tcggctgcaa atgttcaccg ctgtcgaggt cgatggtctt
    13141 gatatgaaac gacaggcctt tttcctgcaa cgccacccag gcggataaca cataagggga
    13201 gaaaaagtgg gcatctgacc aaagcgtgat agcgggttta ctcattatgt cctcggcaat
    13261 aaagggggct ggctatcaaa ctatagagcc tttttgtcac tgtcacgtga cgaatactca
    13321 cgtaagtcgc cgcgctttct atacttgtgg tgttcagatg attaaacaac cggagctgca
    13381 atgatcgatc tctatttcgc cccgacaccc aatggtcaca aaattacgct gtttctcgaa
    13441 gaagcagagc ttgattatcg cttgattaag gtagacctgg ggaaaggcgg tcagtttcgt
    13501 ccggaatttt tgcgcatttc gcccaacaac aaaattccgg caattgttga tcattctcct
    13561 gccgatggcg gcgaaccgtt aagcctcttt gagtctggtg ccattttgtt gtatctggct
    13621 gagaaaacag gactcttttt gagtcatgaa acgcgtgagc gcgccgccac attacagtgg
    13681 ttattctggc aggtaggcgg actggggccg atgcttgggc aaaatcatca ttttaatcac
    13741 gcagcccccc aaaccattcc ttacgctatt gaacgttatc aggttgaaac tcagcgtctt
    13801 taccatgtac tgaacaagcg gctggaaaac tcgccctggc tgggaggcga gaactacagc
    13861 attgcggata ttgcctgctg gccgtgggtt aatgcctgga ctcgccagcg aattgacctc
    13921 gcaatgtatc cggcagtcaa gaactggcat gagcggatcc gttcgcgccc tgccaccggg
    13981 caggcactgc taaaagcaca actcggtgat gagcgttcgg atagttaaca gaaacaggtt
    14041 ctcgtgtatt atttcatcct aagtaaaaca acggagaacc tgcaatggca caacctgccg
    14101 ctattattcg tataaagaac cttcgtttac gtacgtttat cggaattaag gaagaagaaa
    14161 ttaacaaccg tcaggatatt gttatcaatg tgacgatcca ctaccccgcc gataaagcgc
    14221 gcactagcga agatatcaac gatgcgctga attatcgcac cgtaacgaaa aacattattc
    14281 agcatgtaga gaataaccgt ttctctttgt tggaaaaatt aactcaggat gtgctcgata
    14341 tcgcacgtga acatcactgg gtgacgtatg ctgaagtgga gatcgataaa ctgcacgcgc
    14401 tgcgctacgc cgattcggta tccatgacct taagctggca gcgttaaccg ccatatcggg
    14461 aggctgcatg aacatagtga tcaccggagg gacgggatta attggtcgcc atttgattcc
    14521 gcgtttgctg gagctgggcc atcaaatcac ggtagtgacg cgtaacccac agaaagccag
    14581 ttccgttctc ggccctcggg tgacgctatg gcaagggcta gccgatcaaa gcaacctcaa
    14641 cggcgttgat gcggtaatca acctggccgg agaaccgatt gctgataaac gctggactca
    14701 cgagcaaaaa gagcgtctct gccaaagccg ctggaatatc acgcaaaaac tggtcgattt
    14761 gattaatgcc agcgacacgc cgccgtcggt actcatttcc ggctcggcaa cgggctatta
    14821 tggcgactta ggtgaagtgg tggttaccga agaggaaccg ccgcataacg aatttactca
    14881 taaactctgc gcccgctggg aagagattgc ctgccgggcg caaagtgaca aaacgcgagt
    14941 gtgcctgctg cgaaccggcg tggtactggc accggatggc ggtattctcg gtaaaatgct
    15001 gccgccgttt cgtcttggcc tgggcgggcc gattggctct ggtcggcagt atctggcctg
    15061 gattcatatt gatgatatgg tcaacggtat tctctggctg ctggataacg agctgcgcgg
    15121 gccatttaat atggtttcgc cctacccggt acgcaatgaa caatttgccc atgcgctcgg
    15181 tcatgcgctg catcgcccgg ccattttgcg cgtccccgcg accgccattc gactgttaat
    15241 gggcgaatct tcagtactgg tattaggtgg acaacgcgcg ctgcctaaac ggctggaaga
    15301 agcgggtttt gcgtttcgct ggtacgattt agaagaggcg ctggcggatg tcgttcgctg
    15361 atgtggttta cagcaaacat ccgccagtta actcccggtg ttacaggatt agtggctttg
    15421 cgctataaga tcgtctggcg atagtcgggt caccatcata actaactctc tgtctaaacc
    15481 tctatccagc atctcctgag caatacgcag ggcttcttcg tgtttgccct gcattgcgcc
    15541 ttcttcacgt aatctgtcag caatggtcat cagtttctcc ttttcttgtg gtgcgcgttc
    15601 tgttatctca ccaataaatg cacggaaacg ctgggcatcc cctgtttgta atacgtaatt
    15661 aaacagggct tttagctgtc tgtcattagt gttccctgta actagcagcg aaacaatttg
    15721 gtcgactaat cccaacagat cgcgctgacg aatatgtttc tgaattaact ccaacagcgc
    15781 cattttgcgg tgttgcataa tctcgtcatc cggcaccacg gtaatatcca ccaacggaaa
    15841 cgctgatgaa tatattttgc gggctatagc aggctcggca aattcatcaa gccagcagag
    15901 tgaataagga taaggacttc tgcaaccatg ataaaacagc atcgggatca ccaatggaag
    15961 ctctttatag cccgcatcaa gatggttttg cattgccgca atggaataac gcatcatgcg
    16021 aaaagccatt aattcttccg gcttactttg gtgctctatc actacataaa tataacccac
    16081 tccctcctgc gttttcacag accacaagag gtcggaataa tattgccgca ggtcgtcatc
    16141 aataaaactg tttggttcca gtttaagcgt cgttaaatca cacagtttgc gcagcggcgc
    16201 gggaagatga atatcaataa aatcccgcgc ggtgtctgga tggcgtaaaa aagatttaaa
    16261 taccgcatca tgcggcgtgg aagttgtcga tattgtcatg gcgttccgtc acccctcaaa
    16321 tttaagatga cgcgacaata accacgatta acacccaatg cagcaggcag tttttatctt
    16381 tggacagcga attcagaagt atttttagcg gactgcaact tttacatttc agcggaagct
    16441 gactcgcaaa ttccccatga aaatactccg gcagcgttag ccaccggaga ggaaattatt
    16501 tcagcgatcc cttaaggaac tgttgcagac gagggctttg cgggttgcca aataactgct
    16561 ccggcgcgcc ctcttcttct attttcccct gatggaggaa aatgacgtga gtagaaacat
    16621 ggcgagcaaa gcccatttcg tgagtcacta ccaccatggt tttcccctct tctgccagtt
    16681 gctgcataat acgtaacact tcgcctacca gttcaggatc gagcgccgag gtaggttcat
    16741 caaacagtaa aacttccggt tccatcgcca gcgcccgcgc gatagaaaca cgctgttgct
    16801 gaccgccgga aagatgcacc ggatatttcc cctgcgcacg ttcgtctatc ccgactttcg
    16861 ccagatactt caccgcccgc tcgcgcgctt cctgcttgct caggcccaac acctgaatcg
    16921 gcgcttccat gacgttttcc agcaccgtca tatggctcca gagattgaag tgctggaata
    16981 ccatcgtcag gcgtgtgcgc agtaagcgca gttgattttt atcggcgact ttgagttgac
    17041 catctttgtc gcgcaccaga ttgatcgtct ggccactgac cacgatcgat ccttcgctcg
    17101 gtttttcgag gaagttaatg cagcgcagaa aggtactttt ccccgatccc gacgatccga
    17161 tgatgcttat tacatctccg gcattcgctt gcagtgacac ccctttcagc acttcgtgtt
    17221 cgccatagcg tttgtgcaaa tcgataacgt ttaatttatt ctcggacatc gtattctcag
    17281 tgcgttgaag aaggtttcac atgctgcaac cagcgttttt ccgctctgcg aaagaggctg
    17341 atcaggacat aagagatgat taaatagagc accgcggcaa tgccgaaggc ggtaaaaggt
    17401 tgatacgtgg cggcgttaat atcgcgggct attttcagca gatccggcac cgtggccgta
    17461 aacgccagcg cagtagagtg cagcatcagg atcacttcat tgctgtatgc cggtaacgca
    17521 atacgcagcg cagaaggcaa aataatgcag cgatacattt taaaggtcga gaagccatag
    17581 gctctggcgg cttcaatttc cccatgcgga accgaacgga ttgccccagc aaaaatctcg
    17641 gtagtgtaag cgcaggtgtt aagcgtcagc gccagcacgg tacagttcag gccactgcgg
    17701 aagaaagcgt taaggaattc ggttccctta acaatctcaa gcgtgtacat gccggaatag
    17761 aacaccagca actgaacata cagcggcgta ccgcgaaaaa tataggtaaa taaccagatt
    17821 ggaaactgga tgtatttatt actggagaca cgaccaatcg ccagaaacag cgccaggact
    17881 ccgcctatca ctaccgacaa aataagcagc cacagagtga tcgccacacc agtaaagcga
    17941 taaccgtcgg tccacagcag cggtttccag tattcatgta agatttcgat cacaggtcag
    18001 ccctcttcac acccacggag tagcggcgct caaggaacag cagcacacca ttggaaacag
    18061 tggtgaaaac caggtaaatc acgccacaga cgatggcgaa atagaacggt tcccaggtac
    18121 ttttgcctgc cagttgggtg gctttgacca catcttccag gccgagtaac gaaaccagtg
    18181 cggtagattt gaggatcacc tgccagttgt tgccaatgcc tggcagggcg taacgcatca
    18241 tcgccggaaa catgatccgc cgaaacactt gcccacgagt aaaaccgaac gccgtcgccg
    18301 cctctatatg tcctttcggc actgccataa aagcaccacg gaacgtttcg gtaaaataag
    18361 caccgtagat aaaaccgaga gtgataatac cagcgaccat cggatcgata tcaatctgcc
    18421 cgacgcccat cgcctccgtc accgtgttta gcgcaatctg caaaccgtag aaaatcagca
    18481 gcatcaacac taagtccggc acgccacgaa tcagcgtggt gtacccttcg aaaatcagcc
    18541 ccgaaagccg attttgcgag agcttaccgc cagcgccaat taaaccgatg attacagcga
    18601 gcactacaga gctgatagcc agctccagcg tgacgagcgc accctgtaaa ataacacctg
    18661 aaaacccata caacatgctg cctgtcctgt cgtgtgtggt gaattaccgc ctcttgtctc
    18721 cctccggtta cccgaaggga gcgagctttc tttcaccgat ggcgattaac cgccataaac
    18781 atcaaaatcg aagtactttt tcgctaattt ctcgtaagta ccgtcagcgc gcatttcggc
    18841 aaaggctttg ttcagtgcct cgcgcagttc gttatcttct ttacgcaggc ccatgccggt
    18901 ccctacacca aacagttttt catctttaac agacgggcca ccgaatttgt aatctttacc
    18961 gacgggttgt ttgaggaaac cttcactggc agcgacctca tcctggaacg cggcatcaat
    19021 acgtccggca gtcaggtcag aataaatgtt gtcctgcccc tgatacgaga cgatttcaat
    19081 gccttttggt gcccagtgtt cgttaccgaa cgtctcctgg gtggtgccct gcaatacgcc
    19141 tacccgcttg cctttcagcg actcgactgt cggctgaatg tcggaatttt tcgccaccac
    19201 cagacgagaa tcggcagcgt acagtttgtc ggtgaaggca atttcttgct gacgtttttc
    19261 cgtaatggaa agcgatgaca tgatggcgtc aatcttcttc gcttttaagg acgggattaa
    19321 cgcatccagc ggattttcga caaacgtaca ttgcgtattg atgcgtttgc ataattcctt
    19381 tgccagatcg atatcgaagc caaccagttc gccttgtgaa ttttttgatt caaatggcgc
    19441 ataggtcggg tcggtaccga tgcggatgtt ttgcggaatc gcagcaaatg ccgcagttgc
    19501 gctggagaag gccagaacca gagagagcga tagcaccagt tttttcataa tgtcctcaac
    19561 tgacagtctt tttataggga atatttacag gtttgttaca cttatcgtgc cataaaattg
    19621 accatcaagg caaaatatcc cgccccgtta gacgtattat ttttcattaa aaatgcttaa
    19681 gtatgcattt caatgcacca cgatagtgca acaaacctgg cgtgcaccat gatgatcatt
    19741 ccatcaggta cagcttccca gcgacgtacc agtcaccgta gacattaaag tcgaaatact
    19801 ttttcgccat cttgtcgtag gtgccgtcct gacgcagctc gccaagcgcc ttattgaagg
    19861 cagccgtcag ttcagcatca tctttacgta gccctacacc ggtgccatca ccgaagtatt
    19921 ttttgtcttt tactgatgag ccagcaaagg cgaaatcttt accagcaggt tgcttgagga
    19981 atccttcgct ggcagcaact tcatcttgta acgcagcatc cagacgtcct gcagccagat
    20041 cggaatagac caaatcctgg ttggcatagg ccaccacatc cacgcctttg ctacgccagg
    20101 tctcgttagc gtaagcttcc tgggttgatc cctgcagcac accaacatgt ttacctttca
    20161 gtgaatccag cgttggctga atcggtgaac ctttggcagc aatcaaacga gaatctgcgg
    20221 cgtacagctt gtcggagaag gcaatctcct gctgacgttt atcggtaatg gaaagcgacg
    20281 aaataatggc gtcgattttt ttcgctttca gtgaggggat cagcgcgtca aagtcactgg
    20341 caacccaggt acatttcacc tgcatccgtt tgcacatctc gttaccgaga tcgatatcaa
    20401 agccaacaaa atcaccttta gcatctttcg acgagaacgg cgcataggtg gtatcggttc
    20461 cgatacgtac cgtctccggt agcgccgcat agctggaagc cgctgcggag agaccgacta
    20521 acaaagacag agcgagaatc gacttcttca tacataaccc tcaagtgaaa atggcttttt
    20581 tatgttgtgt attgtgttgt gtgtttgcag gctctttcat gcaggtctta tgccatcttg
    20641 acgacaacag taacattcaa cgttaaatat gttaataaga cgttgcatta ttgtcctgaa
    20701 gttgaagata gcaggtatag cggttgaatc gcagcgtttc gattgaatgg cagaaacaaa
    20761 atgtcgagga tttgatcgcg gttgcaaaat tgccctgaaa cagggcaaca gcggagttat
    20821 gcgccctgcc agcgggcaaa gagatcttca ggaagggtta tcgcaaactg gtcaagaaca
    20881 cgattaaccg tctgatttat cacatcatca agggattgcg ggcgatgata aaacgccgga
    20941 acgggaggca taatcaccgc accgatttct gccgcctgag tcattaaacg cagatggcct
    21001 aagtgcaatg gtgtttcacg cacgcagagc accaacgggc gacgctcttt cagcaccaca
    21061 tctgccgcac gggtcagtaa gccatcagta tagctatgga caatgccgga aagggttttg
    21121 attgaacagg gtaaaatcac catccctagc gtctggaaag aaccggaaga gatgctggcg
    21181 gcaatatcgc gcgcatcgtg cgtgacatcg gctaatgcct gcacttcgcg cagagaaaaa
    21241 tccgtttcga gggataaggt ctggcgcgct gcctggctca tcaccagatg cgtttcgata
    21301 tctgtgacat cgcgcagaac ctgtaataag cgcacgccat aaatcgcgcc gctggcaccg
    21361 ctgatgccta caatgagtcg tttcataaat agttgccctg tcagacttgc aggcagactt
    21421 tgcaggattt cggcgggagt tgcaagtcag ggtgccagac cggcaccctc agcgaaggca
    21481 tcatccttcg ttatgcattt cgagattttc cacttcgttc tgacgttgca ctgctttggc
    21541 gtcatcatta cgtaacgtat cgaggaaatc gaggtagccc tgatcaacat ctttggtgac
    21601 gtagacgccg ttgaacaccg agcattcaaa ctgctggata tccggatttt cagcgcgaac
    21661 ggcgtcgatc agatcgttca gatcctggaa aatcaacccg tcagcaccga tgatctggcg
    21721 aatttcatca acttcgcgac cgtgagcgat cagttccgtg gcgctcggca tatcaatacc
    21781 ataaacgttc gggaagcgaa tttccggtgc cgcagaagcg aggtacactt tcttcgctcc
    21841 ggcttcgcgt gccatctcga taatctgctc agaagtggtg ccacggacga tggagtcgtc
    21901 gaccagcagg acgtttttat cgcggaactc ggcgcggttg gcattcagtt tacggcgcac
    21961 ggacttacga cgcagctgct ggcccggcat gataaaggtg cggccaacat agcggttttt
    22021 aacgaagccc tggcggtacg gtttgcccag aatacgagca atttccagcg cgatatcaca
    22081 cgaggtttct gggatcggga tcaccacgtc gatatccaga tcttcccatt cgcgggcaat
    22141 tttctcgccc agtttcgtgc ccatattcac acgcgcgctg taaacggaaa ttttgtcgat
    22201 gaacgagtcc gggcgagcaa agtatacata ctcaaacagg cacggattgc tgaccggatt
    22261 gtcagcacat tgacgggtaa acaactgccc ttcttcagtg atgtaaatcg cttcgcccgg
    22321 cgcgacgtca cgcaggaaat caaagcccag cgtatcgagc gctacgcttt cggaagcgac
    22381 catatattct gtacggttct cgtcaatatc acgttttccc agtaccagcg gacgaatccc
    22441 gtttggatcg cggaaagcaa ccataccgtg gccgataatc atcgccacac aggcatatgc
    22501 gccgcggatt aagcggtttg tggcagcaat ggcagcgaaa atattgtcgg cttccagcgg
    22561 gtagtggcgg aagttgtcca gctcgctggc gaagatatta agcagaattt ccgagtcgga
    22621 agtggtgttg atgtggcggc gtttttcttc aaacagtttt ttacgcaact cgtgagcgtt
    22681 ggtcagattg ccgttgtggg caagcgtaat gccatacggg gagttaacgt aaaacggctg
    22741 cgcttcagag gcgctggagc tgccagccgt agggtaacgc acatgaccaa tgcccatatt
    22801 gccctgcaaa cgctgcatat ggcgagcttc aaatacatcg ctcaccagcc cgttcgcttt
    22861 acgcaaacgg aagcagttat tggcatctat ggtgatgatg ccggcggcat cctgaccgcg
    22921 atgctgaagc accgttaagg catcataaat cgactggtta accggcataa caccggcgat
    22981 accgacaata ccgcacatac gtctttttcc tcgttaagcc acatctcaga gcacttacgc
    23041 tctgggcaag aaacttgacg agctttgcag ataatcaaaa aaccatctga tgataaaact
    23101 gaactgcggg atcagctgtg atttgctcca gtcttcgctt ttcgacaccc cggtaaagga
    23161 gtcgagaaag aatagaatgg cggcaacaat caacacaccg cgcaacgcac cgaaacagac
    23221 gcccagcacc cgatcggtgc ctgacaaccc cgttttctcc accagctggc ctatcacgaa
    23281 gttcacgata gcaccaacga tcagggtagc gataaatagt accgcgatgg caatcccatt
    23341 tcgaaccagt tcgtcttcaa agcccgtaaa ccagactgac aggtaagtgt agtaatgact
    23401 ggcaacaaag aaagcacaac cccatgtcac cagcgataac gcttcacgaa caaagccgcg
    23461 gatcaggcta accagagagg aaaaagcaat caccgcgatt atggcgtaat caatccagac
    23521 catatgtgtc ccacgatttt acgccctgtc atcctgttcg gggcgcattc taacagaaaa
    23581 agaaaacgtt tgcgtaggga tttccttccc gcgcatcaat aaaaatggcg ctgaaaaaat
    23641 attcaacgcc atcgactttt tatgcctttg cggcatcgtg caatgcgtgt cggatgcggc
    23701 gtaaacgcct tatccgacct acggttctaa cactgcccag gcctgataag acgcgccagc
    23761 gtcgcatcag gcaagactgt attaattcgg cgtatagccc attaccacgc cacttaagcc
    23821 agaaagttgc ttcaactcac ccagcgaacc tttcagctta tctttcgagg catccggccc
    23881 aaccagaata cgggtaattt taccctgcac tggcgtggat ggcgacgtat aaacccgata
    23941 accggcaccg cgcagcttac cgacaatctc attcacttta tcggcatttt tcagcgcacc
    24001 cagttgcaca acataggctt tacccgttgg cgctgctttt tcttccacga ccggcttcgg
    24061 ttctggcttc ggcgcaggtg gtgcttcaac cttcggttta ggcggctcca ccggtttcgg
    24121 cttcggtggg gcgaccggtg caggttccgg ttcaaactcg gtgttattgg ctgcaatagt
    24181 ggccggatcg agcgacggtg ctgcggcatc acctgcccgc acctcttccg ctgcgccttc
    24241 cggcggctgc gtcggcaacg cctgggtggc ggctggcatc atatcaggct catcacgatc
    24301 gcccgctttc ggcaccagcg ggatagccgc gaactcatcc tgataatgtt ttttctgccc
    24361 gtccagcagc cctggaagta caatcacccc cagcgccacc agcacgatcg tgcccactaa
    24421 ccgattctga aacttacttg ccaccgcttc tcctcgcgtc aatcacttcc atgacatgtg
    24481 cgaccgtgtg gaaagaacca cacaccagca cggtgtcttc cgctttagcg tccgccattg
    24541 cggcatccca tgcctgcgca acgctatcaa atgatttgcc gttacccaaa tgctcaagca
    24601 gttgttctgc cgtggcaccg cgcggccctt ccagtggcgc acaataccag tcatcaacca
    24661 cgcttttcaa ccaggccaga gttccggcaa tatctttatc atgtagcata ccgataaccg
    24721 ccagcacgcg cccgtttttc ggtagcgctt tcatacgccc ggtgagatat tccgccgcat
    24781 gtggattatg cgcgacatca aaaataacgc gtggcgactc gctcacaatc tggaaacgtc
    24841 ccggcaaaat tgcgctggca atcccgtcgc gaatggcatt ttcactgact tccagcccgc
    24901 tggcacgcag tgccgccagc gctgttgcgg cattcggttg cgggacaagc ggcaacggca
    24961 gattttccag cgtgccgtga gcatcgctaa acgcccagtc atgatcggtg acggaatagt
    25021 tccactcaac gccccgacgt tgtaacagtg cacctttttc ctgcgccaca tcagcaatgg
    25081 tagaaggcat ttccggctca ccgacaattg ccggttttgc gctgcgaaag atgcctgctt
    25141 tctcgcgacc aatactttcg cgatctggtc ccagccagtc ggtatgatcc agcgcaatgc
    25201 tggttactac agccacatcg gcatcgacaa tattggttgc gtccagacga ccgcccagcc
    25261 ctacttccag aatcactacg tcaagttgtg cctgtttgaa cagccacaac gccgacagcg
    25321 taccgtactc gaaataggtc agggaaatat caccgcgtgc cgattcaatc tccgcaaaag
    25381 aggcggtgtg ggccgattcc ggcaattcct gcccctgcac acgtacgcgc tcggtataac
    25441 gcaccagatg cggtgaactg tagacgccca ctttgtaccc tgccgccatc agaatcgact
    25501 ccagcgtacg gcaggtggtg cctttgccat tcgtacccgc aacggtaaac acaaatggcg
    25561 ctggtttcag gacgccaaga cgcgccgcga cctggctcac gcgctcaagg ccgagatcga
    25621 tagttttact gtgcaggttt tccagataag aaagccacga agccagaggc gacgcggctt
    25681 gaggagtgcg tttgataatc atggtatccg ctgattcgtt acggtgagaa tagcaaaagg
    25741 gcagagccag tggccctgcc cttatcagtt atcaggcctc aggttcctga tccggtaccg
    25801 ggggtaccac tacgccttca cgcggcgctt caggattcgg cgctggcaga ttcatcaact
    25861 tcgccagaat gctcgccagt ttcaggcgca tttccggacg acggacgatc atgtcgatcg
    25921 cgcctttctc gatcaggaat tcactgcgct ggaatcctgg cggcagtttt tcgcgaacgg
    25981 tctgttcgat aacacgcgga ccggcaaagc cgattaacgc tttcggttca gcgatgttga
    26041 gatcgcccag catggcgaaa cttgcagaaa caccgcccat cgtcgggtcg gtcagcacgg
    26101 agatgtacgg caagccgcgc tcctgcattt ttgccagtgc cgcagaggtt ttcgccatct
    26161 gcatcagcga catcagtgct tcctgcatac gtgcgccacc agaggcggag aagcagatca
    26221 gcgggcagtt atcttccagc gcctgctcaa cggcacgcac gaaacgtgca cccacaacag
    26281 accccattga accgcccata aaggcgaact cgaatgccgc agcgacaacc ggcattccat
    26341 acagagtgcc tttcatcacc accagcgcat ctttttcgcc agtttctttc tgcgcagatg
    26401 ccagacggtc tttatacttc ttggagtcac gaaacttcag cacatctttc ggctcaagct
    26461 cgctacccag ctccacaagg cttccttcat ctaacaggct atgcaggcga ttacgcgctg
    26521 tcatacgcat gtgatggtca cacttcggac agacctcaag attacgttcc agctcagcgc
    26581 ggtataaaac ctgaccgcag ctatcacact tagtccacac cccttcagga atgctcgcct
    26641 tgcgggtggg agtaatgttg cttttaattc gttcaatcca gctcattagg gacctttctg
    26701 tctgaacctg gttcgatgcc agttttatct ttggggacgc ataatgccat ttttgccccc
    26761 aacagaccat gaatgttgca cattaaaaca taacagcccg aaactttgga taaaaaagtg
    26821 gtcgaaccgc ggagttactt tttattttgc ggcgcgtgcc gcagcgcgtt tgtgacgaat
    26881 aaattcgaaa accccaggca atattgaaac aaaaataatg ccaacaatca tcagcttaag
    26941 gttgctctga atgaagggga gcgtgccgaa gaaataaccc gcgtaggtaa aaatcagtac
    27001 ccacaacagt gcgccgatca cgttataagc ggcgaaatga cggtacgaca tgtggcccat
    27061 tcccgcaaca aacggcgcga acgttctgac gatcggtaca aaacgggcga gaataatcgt
    27121 tttgccgcca tgtctctcgt aaaactgatg agtcttatca agataactgc gacgaaagat
    27181 tttcgaatcc gggttactga acagtttgtc accaaataac cgtccaatgg tatagttcac
    27241 tgcatcaccg acaatagcgg caatcaacat aagcaccacc atcatatgga cattgagatc
    27301 gttagtttcc agcgacgcca atgcaccagc aacaaacagc agcgaatcac caggcaggaa
    27361 tggcgttacc accagccccg tttcgcagaa cagaatcaaa aacagaatgg cataaaccca
    27421 gacgccgtac tccgcgacca gctctgccag atgcacgtca atgtgcagga taaaatcgat
    27481 gaggaaataa atcaggtcca tatttgccta tgccttgtac tcgtcatctt tcaggctgta
    27541 actacgttgg ctacagcctg aaatgttccg agcattattc tcgttagtcc gccagaaata
    27601 gcgggcccat tggcggtttt ggaagatcat accggtcagg gtaatccacc gcgaccagat
    27661 acagcccttc cgcttttgcc gttgctgccg ccagcgttct gtcctttgcc gccagcaact
    27721 ctgctatcca gctctccggc tggttgtggg caccgacttc catcaggctg ccgacaatat
    27781 tcctgaccat atgatgtaca aaggcattcg ctttgatatc taccaccaca taaggaccgt
    27841 gacgcgtgac attaatatgc ataacgttgc gccacggggt tcgggactgg cactgcaccg
    27901 cacggaacga ggtgaaatca ttctcgccca gcaagcattg cgcagcccga tgcatccgtt
    27961 cagcgtccag cggttcgtaa aaatgggtta cccctttact cagtaccgcc gggcgcagcc
    28021 gatgattgta gatgatgtag cgataacggc gagccgtggc gctaaatcgg gcatgaaaat
    28081 catcaggtac agctttaacc caacgcacgg cgatgtcacc aggtaaattc gcatttacgc
    28141 ccaaggtcca cgccgcgtct ttgcgctgcg cggtcgtttc gaaatgcact acctgcccgg
    28201 taccgtgtac accagcgtca gtacgcccgg cgcagaagac ggtaatgggt tcgttcgcca
    28261 ccagggagag cgccttttcc agcttctcct gcacactgcg gacttcattc tgccgttgcc
    28321 agccgtaata cttactgccg tcgtactcaa tgcccagcgc aattttataa actggcggtt
    28381 gttgctggtc ggacattagt acagatactc ctgcaccagt ttctcggcga tttttactgc
    28441 catcagcgcg ccgccaaagc gaacgttatc ggccaccgac cagaactgga cttgctccgg
    28501 cataccgtag tcattacgca cgcagccaac agaaagatgc ggcgtacccg aagcatcccc
    28561 tacctgagtc gggaattcgt tctcttcaga gagcacaata tcttcgccct gagcaaacgc
    28621 atcacacgct tcttctgccg ccagcggacg cagtgcttca aagttgacca tctgggcatg
    28681 accgtagaat accggtgcct ggacgacgct tgccgaaatc atcagccctt cgtcctgcag
    28741 gattttgcgt acttcgtcaa cgatacgacg ttcttcacgc acgctacctt cgctatccgg
    28801 caacaacggc agcatattga acgccagttg acgaccgaag aaatcttctt cgtcaatcgg
    28861 aatgccgttg agcaatttcg cactctgccc cgctaacgca tcgaccgctt ttttgccctg
    28921 ggcggaggct gaaatcaggc tggtaacact gatacgcgac aaaccgccct gatcgattaa
    28981 cggtttcagt gccgccagca gctggctggt cagactgtct ggtacggcga tgacattccg
    29041 gttgcggtaa tcggtcagta caaacgggtt tacttccggc accaccagcg gtacgtcggg
    29101 ttcgagagca aacagtccgc tgctgtcgat caccaggcaa cctgagttgg tcgcttcttc
    29161 aacccaggca gcggtagctt ctttgcctgc gacaaaaaat gccagctgcg cctgcgtcca
    29221 gtcgaattca gcggcatcct gcacggtgat tgtcttacca ccaaagcgca gttgttcgcc
    29281 tgcgctttcg ttacgtgcca gtgcataaat ttccccaacc gggaactgac gttcagccag
    29341 cgtttcaagc agggcttcgc ccacagcgcc agttgcgccc aggacggcaa tgttccagcc
    29401 ttcagacatg gtggtttact ccagaaatag caaagctccc tgccaatgtt acagcagaga
    29461 gcatgaagaa gagattaacg tgccggatga tgaacggcgt taaaacccag tttacacagc
    29521 aatgatgccg cactggcgtc atcacaaatt acatacagag atgaccattc acggcgctca
    29581 agatagtttt tgcgcagttt atcgaactca cccggtatcc cggcgacttt acgcagcggt
    29641 gcgtcatcgc ggcgcacatc atacaccaaa tgcaccagcc ttttcagcgt cggttgatcg
    29701 agcgggccat gcagcgtaat gcgaccaaac tctggcgcgg gcagtaatgt atccagcgca
    29761 acgtgctgtt catgcccaat aaacttgcta taggcttcaa acacttgcgt agtaccgcgt
    29821 gctttacctt ccagggtata acctgcgata tgcggcgtgc cgatatccac ttttgtcagc
    29881 aactccacgt taagttccgg ttcgccttcc cagacatcca gcactacgct taacttctgg
    29941 ccttcattca ggcaggtcag caacgcagta ttatcgacga ctgcgccacg gcaggcgtta
    30001 atcagaatcg ctcccggctt caggctacgg atcagttttt catccgccag atgcagcgtt
    30061 ttgtacggcc catctttgaa gagtggcgta tggaaagtca gaatatccgc atgctgaact
    30121 aattcatcca gcgagcggaa atcaccctca tccccacggt cagcgcgagg cggatcgcaa
    30181 agcaaggttt taatccctaa cgcttccagt cgcgcttgca atcgacgtcc aacgttacca
    30241 acgcccacga tccccaccgt acggtcgtgc agtgaaaatc catcgcgttc ggcaagcatc
    30301 agcagggagg agaaaacata ttccaccacc gcaatcgcat tacagccagg tgccgcggaa
    30361 aaaccaattc ccgcctgctt taaccatgct tcatcgacat ggtcggtccc cgctgtggca
    30421 gtaccaacaa atttaatggg ttttcctgcc agcaaagatt cattcacttt cgtgaccgaa
    30481 cgcaccatca gcgcatccgc gtctgccagt tgagcgacgg ggattggacg cccgggaacc
    30541 gcggtcacct cacccaaacg gctaaataag tcgcgggcat aaggcatatt ttcatcaaca
    30601 aggattttca cgtttgtgtt acctgtatga gacgagagtt aaccggacaa gtgtgccata
    30661 atctcgcggc caggcatact tgcgaagatt tcaggtataa ggatacgtaa tgatacaacc
    30721 tatttccggc cctcctcctg ggcagccacc aggtcaggga gataacctgc cgtctggcgc
    30781 gggcaatcag cctttatcca gtcagcaacg tacttcgctg gaaagcttaa tgacgaaagt
    30841 gacctcactg acgcaacagc aaagagcaga actgtgggcg ggtatcaggc acgatattgg
    30901 tctgtcggga gattcaccgc tgctttcgcg tcacttccct gccgctgaac acaacctggc
    30961 gcaacgtctg ctggccgcgc aaaaaagcca ttctgcccgc cagcttttag cgcaattagg
    31021 ggagtattta cgtctgggga ataatcgtca ggcggtcacg gattatatcc gtcataactt
    31081 tggtcagacg ccgctgaatc agctctcacc ggagcaatta aaaaccattc tcaccctgtt
    31141 gcaggaaggg aagatggtta ttccgcaacc gcagcagcgc gaggcgaccg accgtccttt
    31201 attaccggcg gagcacaatg cgctcaaaca gctggtgacc aaacttgcgg cggcaacggg
    31261 ggaacccagc aaacagatct ggcaatcgat gctggaactt tccggggtga aagatggcga
    31321 gttaattcca gcgaaactgt ttaaccatct ggtgacctgg ctgcaggcgc gccagacgtt
    31381 aagccagcaa aatacgccga cgctggaatc actacagatg gcgctaaaac aacctttaga
    31441 tgccagtgaa ttggcggcgt tatcggcata tatccagcaa aaatatggcc tttctgcgca
    31501 atcatcgctt tcttctgccc aggccgagga tattcttaat cagctttatc aacggcgggt
    31561 taaagggatt gatccgcgtg atatgcaacc gctgcttaat ccttttccac cgatgatgga
    31621 cacgttgcaa aatatggcaa cgcgtcccgc gctgtggata ctgttagtcg cgattatcct
    31681 gatgctggtc tggctggttc gttagccccg acgaaatgac agtatcgtga caataattcc
    31741 cagcaccgca gagatcgccc cggcaagaaa taccgaagag taaccaaacg tggtcgccag
    31801 cattcccgcc agcggcccgg agacgccgag ggcgatatcc tgaaacgcgg cgtaaccgcc
    31861 cagtgcggtg ccgcgaactt gtgaggggac gcgtttaacc acctccacgc ccagcgcagg
    31921 aaagataagc gaacatccgg ctccggttaa cgccgcgccc gctaatgcga cccacgcccc
    31981 tggggcttgc cagagcagca acaagcccac cgtttctaca agcagagaga caatcgccac
    32041 tttcacgccg ccaaaacggt ccggcatcca gccaaacatg acgcgcatca cgacaaatgc
    32101 gccgccaaac gcggtaagag taaagcccgc catcgcccat cctttgctgg caaagtagag
    32161 cgaaacgaaa gtcccgataa ccgcaaaacc aacgccttgt agtgccagcc ctaaccctgg
    32221 tttccagata agcccgacaa cgctccacag cgatggacgt tctcccgcca aggccggtac
    32281 tttgcgcact gtgccgttac aggcccatgc cagtaagggt aataccattg tggtgatcgc
    32341 cagtgcggca aaaccgtaat ggctatgaat caacaggcca agcggagcac caacagcgag
    32401 ggcaccgtaa atcgccattc cattccatga catcactttg ccagagtgtt ttggccctac
    32461 gatgcctaac ccccaggtca gagcgcctgt cagtaactgg ctttcaccaa agccaagaat
    32521 caaacgcccg acgaccaaca gggcaaattt gaacggtgcg gagacaggca aaatcgccgc
    32581 cagcagcaac gcgccgccag ccagaccaca agctaacatc ccctgaagcg ccgaacgttt
    32641 tgcaccatat tgatcggcca gtcgcccggc atagccacgc gtcagcaccg tagccagaaa
    32701 ctgaatcccg acggcaatcc cgaccatggt attgccatag cccagatcat gatgaacaaa
    32761 cagcgggata accggcaacg gcagccctac ggtcatgtag gtgagaaaaa ccgcaaaagc
    32821 gatgcggaag agcgaaaaat tggcagaaga tcgtgtttcg gtttggctta cagcagtcat
    32881 gcattactcc agaatgcagc gcaaggcgag gagtatcccc gtctcatctc tctggtttca
    32941 gggttacggt gcgttggcag gatttaacgc gtacgtcttt tcagaaggaa atcgacaaag
    33001 cgggaagttt gcctggaaca ggcggcgatt gtcaatgatg tgaaaaaggg aaccatcagg
    33061 ttcccttttg cgttagtgcc ggaggccgca ttagcgcgta acgtcggatg cgacgctggc
    33121 gcgtcttatc cgacctactc cgactactcc gacctactgc gaattaatct ttcagcttgc
    33181 gcattaccag cgtggcgttg gtgccgccga agccgaagct gttagacata acggtagtca
    33241 gttcgcgatc ggtcgtttcg gtcacgatgt tcagacccgc agcctgctcg tccagctctt
    33301 caatgttgat gctcggggcg ataaagccgt gttccagcat cagcagagag tagatagctt
    33361 cctgtacgcc agcagcgccc agagagtgac cggtcatggc tttggttgca gaaatcgccg
    33421 ggctcttatc gccgaacact tcacggatag ctgccagttc tttcacgtcg ccaaccggag
    33481 tcgaagtacc gtgagagttc aggtaatcga ttggggtatc aacgccatgc atcgccatct
    33541 gcatacaacg tactgcgcct tcgccagacg gagcaaccat gtctgcacca tcagaggttg
    33601 cgccgtagcc aacgatttca gcatagatgt gagcaccacg cgccagcgcg tgttccagct
    33661 cttcaaccac taccataccg ccgccgccag cgataacgaa accgtcacgg tgagcgtcgt
    33721 aagtacggga ggctttttcc ggggtgtcgt tgtatttagt agacagcgca cccatcgcgt
    33781 cgaattcgca ggccatttcc cagcacagct cttcgccgcc gccagcaaac acgatgtcct
    33841 gtttgcccag ttggatctgc tctactgcgt taccgataca gtgtgcggaa gtcgcacacg
    33901 cggagctgat ggagtagtta acgccatgaa ttttaaacgg cgtggcgagg caggcagaaa
    33961 cgccggatgc catcgctttg gtgaccacat acgggccaac cgctttcagg ccgcgcgggc
    34021 cgcgcattgc gtcagcgccg aatacctgga aacgcgggga accgccgcca gaacctgcaa
    34081 tcaggccaac gcgcgggtta ttctggtaag cttccggaga aaggcccgca tctgcgattg
    34141 cctgctccat agaaaggaat gcataaatgg atgcgtcgct cataaagcgc acaactttgc
    34201 ggtcaatgag gccagtggta tccagtttta cgttgcccca gacgtggcta cgcatgccgg
    34261 aatccttcag ctcctgagag aaagtgatcc ctgaacgtcc ttcacgcaga gatgccagga
    34321 cttcctgctg gttattaccg atgctggaaa caatgcccag gccagtaatc actgcacgtt
    34381 tcattcaata cctctgtaag tcgcacatag agtaagtttc gaatgcacaa tagcgtacac
    34441 ttgtacgccg aacaagtccg atcagccatt taatagagaa atttgcgcag ccttacacac
    34501 atcgctaaga tcgagccacc gcctgtaaga cgagtaactt acgtgaaaca ctactccata
    34561 caacctgcca acctcgaatt taatgctgag ggtacacctg tttcccgaga ttttgacgat
    34621 gtctattttt ccaacgataa cgggctggaa gagacgcgtt atgtttttct gggaggcaac
    34681 caattagagg tacgctttcc tgagcatcca catcctctgt ttgtggtagc agagagcggc
    34741 ttcggcaccg gattaaactt cctgacgcta tggcaggcat ttgatcagtt tcgcgaagcg
    34801 catccgcaag cgcaattaca acgcttacat ttcattagtt ttgagaaatt tcccctcacc
    34861 cgtgcggatt tagccttagc gcatcaacac tggccggaac tggctccgtg ggcagaacaa
    34921 cttcaggcgc agtggccaat gcccttgccc ggttgccatc gtttattgct cgatgaaggc
    34981 cgcgtgacgc tggatttatg gtttggcgat attaacgaac tgaccagcca actggacgat
    35041 tcgctaaatc aaaaagtaga tgcctggttt ctggacggct ttgcgccagc gaaaaacccg
    35101 gatatgtgga cgcaaaatct gtttaacgcc atggcaaggt tggcgcgtcc gggcggcacg
    35161 ctagcgacat ttacgtctgc cggttttgtc cgccgcggtt tgcaggacgc cggattcacg
    35221 atgcaaaaac gtaagggctt tgggcgcaaa cgggaaatgc tttgcggggt gatggaacag
    35281 acattaccgc tcccctgctc cgcgccgtgg tttaaccgca cgggcagcag caaacgggaa
    35341 gcggcgatta tcggcggtgg tattgccagc gcgttgttgt cgctggcgct attacggcgc
    35401 ggctggcagg taacgcttta ttgcgcggat gaggcccccg cactgggtgc ttccggcaat
    35461 cgccaggggg cgctgtatcc gttattaagc aaacacgatg aggcgctaaa ccgctttttc
    35521 tctaatgcgt ttacttttgc tcgtcggttt tacgaccaat tacccgttaa atttgatcat
    35581 gactggtgcg gcgtcacgca gttaggctgg gatgagaaaa gccagcataa aatcgcacag
    35641 atgttgtcaa tggatttacc cgcagaactg gctgtagccg ttgaggcaaa tgcggttgaa
    35701 caaattacgg gcgttgcgac aaattgcagc ggcattactt atccgcaagg tggttggctg
    35761 tgcccagcag aactgacccg taatgtgctg gaactggcgc aacagcaggg tttgcagatt
    35821 tattatcaat atcagttaca gaatttatcc cgtaaggatg actgttggtt gttgaatttt
    35881 gcaggagatc agcaagcaac acacagcgta gtggtactgg cgaacgggca tcaaatcagc
    35941 cgattcagcc aaacgtcgac tatcccggtg tattcggttg ccgggcaggt cagccatatt
    36001 ccgacaacgc cggaattggc agagctgaag caggtgctgt gctatgacgg ttatctcacg
    36061 ccacaaaatc cggcgaatca acatcattgt attggtgcca gttatcatcg cggcagcgaa
    36121 gatacggcgt acagtgagga cgatcagcag cagaatcgcc agcggttgat tgattgtttc
    36181 ccgcaggcac agtgggcaaa agaggttgat gtcagtgata aagaggcgcg ctgcggtgtg
    36241 cgttgtgcca cccgcgatca tctgccaatg gtaggcaatg ttcccgatta tgaggcaaca
    36301 ctcgtggaat atgcgtcgtt ggcggagcag aaagatgagg cggtaagcgc gccggttttt
    36361 gacgatctct ttatgtttgc ggctttaggt tctcgcggtt tgtgttctgc cccgctgtgt
    36421 gccgagattc tggcggcgca gatgagcgac gaaccgattc cgatggatgc cagtacgctg
    36481 gcggcgttaa acccgaatcg gttatgggtg cggaaattgt tgaagggtaa agcggttaag
    36541 gcggggtaat ctgctctcgc agtgtttacc ggatgcggcg tgaacgcctt atccgaccta
    36601 cgtgtgacgt gtaggcctga taagacgcga taagcgtcgc atcaggcatg gtgctccaga
    36661 cgccgcaact tactgctgtg acgcctgctg aaacaggttt tcccacatat cggtcaccag
    36721 cgcctggtca cgcggcgaca attcaccggc accaatggct ttttccagac tctggctaac
    36781 ggtcgtatgc accgcctgag cggagtggtc atcaccactt tccagttctg cgatggctaa
    36841 tgtcaggtgg ccacgcaaat acccactggc aaacaactca tcatcactgg catggtcaac
    36901 cataccgtcg attaatgcca gaatgcgtga ttcaaactcc gcgatcatct tctttcctca
    36961 ttgtaaaacg tggcgctggc tttagttgag cgcttccggc cacgggaact gttccgccgt
    37021 aagttccggc gtgtgataat aattttgtag tgctttaatg aagcgtgccg ggcgttcagg
    37081 aatgccgttt gctaggtaat ccatcacctg cgcatgtacc cgccgttgaa acaccacacg
    37141 atccggttcg aagtcgcctt ccagattgtc gcaactgacg ttaaacggat atcccgccgc
    37201 cacacagaac aaccagtcga gtgcctgcgg cttcacttca acatcttcaa actggctttg
    37261 cgtttgggca tcacgcccat ccgggcaata ccagtagccg aaatcaacca gctcacggcg
    37321 cgcttttccg gcaatacacc agtgcgaaat ctcatgaatt gcgctggcat aaaagccgtg
    37381 ggcaaagaca attcggttat acggtacttc cgcatcagca ggaagataga tcggttcgtc
    37441 gtcgccttta atcagacggg tattaaattc atcggcaaag cagctattaa aaatttcaat
    37501 caactgctca tagtggtgtg tactgttcat tagttcatcc ccaaccagtg gaggatctcc
    37561 tgtccgtggc tatcgtaaag taatttggca ctcatcaccg ccgagacgat aacaatcatc
    37621 gggcggatca gcttttgtcc tttgctcaac accagccgtg aacccatgcg cgcgccgagg
    37681 aactgcccca ccagcatgac aaagcccgtc gcccaaatca ctttgccgcc gagaataaac
    37741 agcagcagac cgccaatgtt tgacgttgcg ttgagtaatt tggcgtgagc cgtggctttg
    37801 gcgaggttga atccgcacag cgtaacgaag gccagcgcgt aaaacgatcc ggcagccggg
    37861 ccaaagaatc cgtcataaaa accgacgcag ccaccggcaa ttaacgcgaa gggtaaaccg
    37921 tacatccggc gctggcgatc ttcttcaccc agctttggca tcagcaagaa atagaggccg
    37981 atacaaatca ccagaatggg caaaatctgc cgtaagacat cagcctgaac gtactgtacc
    38041 aacagtgcgc cgctcattga gccgacaaag gtcatggcaa tattgagttt ctgatcgctt
    38101 aaactaacca ctttgcggcg aataaagtag atagtggcgg aaatagagcc gccgcaggct
    38161 tgcagtttat tggttgccag cgcattagcg ggagacatcc ccgctgccat caatgccgga
    38221 atggtgagta acccaccgcc accggcaatc gagtcgataa atcccgccag catggcgaca
    38281 aaaaagagaa ctcccagcaa cagcggggaa accataaaca ggctattaaa cgtttccatt
    38341 agatcacgtg ctcatccagt agcgcctggc aggaaggcgg caacggaggc ggtgtcttct
    38401 tctcaggctt tgttgttccc ggttttggag gtgcaaacca gctttgcagt tctgccccgc
    38461 aaccatcgcc tggtggcggt aaaggttgat cttcacactc cagactatcg gcaggacaac
    38521 gtaatcgtac atgcatatgc gcgcgatgct ggaaccaggg tcgcactttg cgcaaccagt
    38581 cgcgatcggt gcccgcatca aggcaaagtt gttgtttaat cgccggatta acaaaaatgc
    38641 gtgtgacgtc tttgtcctgg gcggcgagtt tgatcaagct gaaaatttct ggcttccaca
    38701 gcgtggagac aacgtgttta ccgtcgcggg aaactaagtc cagtgcttgc gggcgcaaga
    38761 gctgcgcgga ggtccagcga gttttcggca gttgcagaaa gatatcgaca tccagtccgg
    38821 tctggtggct ggcatgaccg ccgttgaaac gcccaccagc gggcatcccc atatcgccaa
    38881 tcagcaccgt acccatgccc agattgctca cctggctact cagacgctgg ataaacatca
    38941 ccagatccgg gtgaccgaaa tagcgacgct gatcggtacg catgacctga taatgttcgg
    39001 actgtatcgg cagcgtgtca gcaccgacga tacagccatt agaaaaactg cctatcgatt
    39061 gtgcgctacc cggcacaggt tgggttattt tttgccacgg cgtcgctgcc aggctagcgc
    39121 tactggcaag cagagccagc agcgcaatcg cggttttgtt cattttttac cagcgtggaa
    39181 tatcagtctt cacatcggca ttttgcgccc gctgccgtaa caggtgatcc attaaaacga
    39241 tcgccagcat cgcttcagcg atcggcactg cacggatccc gacacaggga tcgtgacggc
    39301 ctttggtgat catctcaact tcttcgccaa agcggttaat agtgcgcccc ggcacggtaa
    39361 tgctggaggt cggtttcagc gccatatgag caatgatttg ctgcccgctg ctgataccgc
    39421 cgagaatgcc gcccgcatgg ttgctctgga aaccgtcttt ggtgatttcg tcgcggttct
    39481 ggctgccgcg cagcgccact acgtcaaaac cgtcgccaat ttccacgcct ttcaccgcgt
    39541 tgatgctcat cagcgcatgg gcgatgtcgg catccaggcg gtcaaagacc ggctcgccaa
    39601 gtccggcggg gacaccactg gcaacaacgg tgactttcgc gccgatggag tcgccctctt
    39661 ttttcagcgc gcgcatcagt tcatctaacg cgtcgatttt gtcggggtcc gggcagaaga
    39721 acggattttg ttcaaccagc gaccagtctt tgatttccag cggaatgtcg cccatctggg
    39781 tcaagcagcc gcggatttca ataccaaatt tctcggcgag atattttttg gcaatcgccc
    39841 ctgccgccac gcgcatggcg gtttcgcggg cggaagaacg tccaccgccg cgataatcgc
    39901 gcagaccgta tttttgttcg taggtgtaat cagcatggcc tgggcggaaa acgtctttaa
    39961 ttgcgctgta atcctgagag cgctggtcag tgttttcgat caacaagcca atgctggtgc
    40021 cagtagtaac gccttcaaaa acaccggaga gaattttgac ctgatccggc tcgcggcgct
    40081 gggtggtata gcgcgatgtc ccagggcgac gacggtcgag gtcatgttgc aggtccgctt
    40141 ccgtcagcgg aatgcctggc ggaacaccat cgacgatgca gccgagcgcc agcccatgcg
    40201 attcgccgaa ggtggttacg cgaaagagtt gtccaattgt gtttccagcc atcacggctc
    40261 cgttattgtt gtgtttgcgt gtttacttaa tctttataaa tcgcgaaata ttcacgggcc
    40321 gccagcagct gctctttggt gagcataaac acaccatcgc cgccgttatc aaactccagc
    40381 caggtgaacg gaacatccgg atattgttcc ataagatgta ccatgctgtt gccgacttca
    40441 caaatcaaca cgccatcatc agcaaggtaa tctgccgcgt ttccaagaat gcgacgtgtc
    40501 agtttaaggc cgtcggtgcc agatgccagg cccagttccg gctcgtgacg gtattcgttt
    40561 ggcaggtcgg acatatcttc cgcgtcgaca tacggcgggt tagtgacaat cagatcgtat
    40621 tgcactttcg gcaaatcacg gaacagatcg gaacgaatcg gaatgacgtt gtggatcaga
    40681 ccgtgttctt cgatgttctg ttcggcaacc gccagcgcgt cgggagagat atctaccgcg
    40741 tcgacttccg cttccgggaa ggcataagca caggcaatgg cgatgcagcc gctaccggta
    40801 cacatatcta aaatatgctg cggttgcttg ctgatgagtc cggcaaattt attgttgatc
    40861 agttcaccaa tcggcgagcg cggcaccagc acgcgttcat cgacgtaaaa ttcatgaccg
    40921 cagaaccagg ctttgttggt caggtaagcc accggaatgc gctcgttgac gcggcggatc
    40981 acgcgttcaa caatacggtg tttttcgctg gaggtcagac gcgcggtgcg catatcttcc
    41041 gggatatcca gcggcaggta gagcgatggt aacaccagct gtacggcttc atcccacggg
    41101 ttatcggtgc cgtgaccgta ccagatattt gccgcgctga agcggcttac cgaccagcgc
    41161 aacatgtcct gaatggtttg cagctcattt actgcttcat caacgaaaat tttatccacg
    41221 tattcctcca gggcatgatc gcaataattt cggcggctag tttgccatga agatgacgat
    41281 aaatcagcat tcacgcgcgg tgagtgagga aaaatacgtt taaaacgatc gattgtgcta
    41341 cgagtcgggt aaactgtagg aaaattagaa atagagacag ataaatgaaa aagaaaacaa
    41401 cacttagcga ggaggaccag gctctgtttc gccagttgat ggcggggact cgcaagatta
    41461 agcaggacac gattgtccac cgaccgcagc gtaaaaaaat cagcgaagtg ccggtgaaac
    41521 gcttgatcca ggagcaggct gatgccagcc attatttctc cgatgagttt cagccgttat
    41581 taaataccga aggtccggtg aaatatgttc gcccggatgt cagccatttt gaggcgaaga
    41641 aactgcgccg cggcgattat tcaccggaat tgtttttgga tttacacggt ctgacgcagc
    41701 tgcaggccaa gcaggaactg ggcgcgttga ttgccgcctg tcgccgcgaa catgtgtttt
    41761 gtgcctgcgt gatgcatggg cacgggaagc atattttgaa gcaacaaaca ccgctgtggc
    41821 tggcacaaca tccgcatgtg atggcatttc accaggcacc gaaagagtac ggcggtgatg
    41881 cggctttgtt ggtgttgatt gaagtggaag agtggttgcc gccggagttg ccctgaatgc
    41941 tggcagcaaa tgccggagca gcgtaaacgc cttatccggc ctacaaatat gtgataattc
    42001 aataaattgc aattcacttc gtaggcctga taagacgcat cagagcaata ctacgaacta
    42061 cagttcattc aatacatact gaaaacgaca cgtcgaatca atcggttgcc cactactggt
    42121 attcacattc accacaatat cgtgtagtac tttatccgtt accggatgac gagttggcgt
    42181 aaccgttacc gttgatttat cacaaccgta agaggtaaag gttaacaatt ctgataccgc
    42241 gcggcagtat gctcctctgc catcattatc agaaaaatta ctgccatcac agagtctgcc
    42301 gccttccatc aagtaggtcg aaagcgtggt ggtcactgtt gaccccgtgg actgaattcg
    42361 taacgcctta ttatggctga cataattttc actccaggag atatcgtatg aagcgaccgg
    42421 tatcgttggg catattgtgt tatgcactgt aggggccgta tagcttccaa atccgggtaa
    42481 atctgtatag gaataaaagg atgcgatcca agggcaacca taatcatcag atgcaacggt
    42541 aattttaaac gtcatatatg cacaacgaaa aattttcgta tctatgccag ggcaatttga
    42601 gttattaatt gtatcaaagt cagtcagtac caaagttgga gctgaacccg acccagacca
    42661 ggaaataacc cgcaacgtat cgcctttata cgcaaaagaa tcatatttcc catatgcaac
    42721 ctcctttggc gttatcaccg taacattgaa aacactgctc gctacagcac cggttacctt
    42781 gtccatggca taataaactc tcacactgtc aaaactcgca ctcataagtt tatctccagc
    42841 ataactctta gatgcgccga caacactcag aacgagcagc attaggctcg cgaaaattat
    42901 ggtccatttt ttcatttcag aaatactcaa aggttgccgt cactgtggca ctaaagtctc
    42961 cggacgttac atctcgcccg ctctttgcct gtaaccaggt attaaaattt aggctgttat
    43021 ttccctgatt cagcgggagc gtgacgcctg ttgtattgtt gattggcacc gatgtgcctg
    43081 ccgcagtttc aataccaatc ccagcgccgg atgctgatga cgaggagtca aaggccagaa
    43141 agccgggtag agcactatct tctgttcctg tcaacgttac tttgacatta aacaatgtag
    43201 aactatgaca atctttcaat tgaaacacca atggcactcg ctctgactgc ccaaggttca
    43261 ttaaatcgct ggcggcaatg gcaggaaaat gtacttctgc caactggcta ccattaatca
    43321 ctggcgtaca cgatttactg attaaattgc cagtaaagtg caaattatct cctgctattg
    43381 tggttccact gcccagcaac atcaggctac taataaaaaa caacgtacgc tttttcttca
    43441 ttggtaatct acctgtaacg tggcccaggc ctcaaagtcc gcctctggca gaacgacacc
    43501 actctttttt actggcacag ccgttaacac cggtaaatcg gtttcattca ccacaagtgg
    43561 cgtattgatc gtaaaactct gccctgcctg tttgagctga atgcctaaac cagtgatatt
    43621 gctgcttact gcagcattat caaaatcgct tggtgtgcca ctccagctca aggtcatttc
    43681 cattaatggg tcgcgaaccg ccgtatcgca ggtaatggaa tacggcacgg tttgctcata
    43741 attgctgcca ttaatgtcat caataagcac atcctgaaaa gttatttcaa tggtttgagc
    43801 gttgctaatc gtgcagttag gtggctgcac taaataaccg tgaaaagaga cgtttgagag
    43861 tgccatcgag caaaagcccc acagcaaaat aagcgagatc cgtttcattg gtaatccacc
    43921 acaattgtgg cactggcgtt aaattcagct gccgctaacg ttgtaccact ctgtttcacc
    43981 ggcacagctt ccagaacggg aactcccgag gaaaaattaa atggcaacca tgagccagag
    44041 gttaagtcga gtattgtgtg atcactactt ttctgaaccc gaataccaaa cccaggaatc
    44101 cccgtgctga taactgtttc accgttaatc acgaccgttg tcgcctgcat ctgtagacgc
    44161 aggtcatcca ttgccagatt gttacaaaca agcgagtagt caatgggacg tttataatca
    44221 accccattga tcttattaat gaatacatta ccaaactcaa ctgccgcacc atttaccgta
    44281 caaggaggag gggcatcgac aagcacccgt aaattcatac ttactgactt actcgctgca
    44341 actgcaaagc cgctaccaac acaacatatc agtaacccag ttaccgccca gacagaacaa
    44401 gatcctttca tactggctct cccggcctca gcctttgcgg ctctgctctt catcgacctg
    44461 gcaactgtta ccgttgcaag tgaaaaagag cggcatccga gcgccatagt cgttgacata
    44521 ggtcagtacc ggcacgctac ccatcttcac attcagcggt gcagaggttt tcggcgacat
    44581 cactattggc gagaaaccgg ctgccggatt gccatttttc tgtgtgctgg cattactgaa
    44641 aatgacgtaa tacggtgtcg ggttattgac ggtaaattcc tgaccgttgc gggttaaagt
    44701 aaccttatat tgccaggggt ttttcatgct gacgttctcc agcgccttcg gtcgccagaa
    44761 caacttaata cgcgtttgta gtgctatttg cagcgtgttg gctttgtttg attttggcgg
    44821 gatctcacgc acgttgaagt agaacaggct ttcacgatcg gcaggcaatt tgttgatatc
    44881 cggcattccc tgcactttta cctgcccgtt catcattgaa tcgatgcgtt gcaccggtgg
    44941 cagaacagtt aacggtgaac tgatcttgtt acccttttca tcttcgatcc agctttgggc
    45001 cagataaggc agtttcggat cgttattgcg caacgtcacg ctgattgatt tgtcgctttc
    45061 attaaatatc aaacgggtac gatctggcgt tacagaagcc tgggcagaca aactggcagc
    45121 gctaagcagt aacgctagcg tcgtagcacc caatttggca gagcaaagta aatcagacat
    45181 ggatcattcc ttaatcacgt tattggttag ctgaaacaga aacaggggga ttggtgggtg
    45241 tcacctgttg cgtctgctcc tgaatgacag gctttatgtc atcaggaaca accggcgcga
    45301 cgttgccttt atgctggcat ggcagtaaca agccgttaaa cagatcggca ggcagcgggt
    45361 ccggcaggtt gatatcgcaa tgcgcaacac cactccagaa cacactcatg tgttcgccag
    45421 gtttcacccc agccagataa acattgccgt catcatcgac aaggcccact gtctgctcgt
    45481 tatcattttt tacttctgcg ccaaacggag gatggctgcc gtcttgtaaa cgcagcaccg
    45541 ccatcgcttt ttgaccactg atgacggcaa atttgcggta gccaatcgcc ccttcagtta
    45601 gcgtggcttg taccaccgac tgggttgctt cggcgttttc cggcagtttg ttgaggtcga
    45661 tatacgcctg attgcggtaa tagttattga cgtcagaaac gacggcttta ccaaacatat
    45721 tggtataaac agcagcaccg ttaccttcta ccggaacatc ggcaacgcca tcggcatcaa
    45781 tcaacaagcg tgtcccgccc atattctggg tacggtgaag tgcgccaccg tgggcagtaa
    45841 gcgttgcgcc gccctgtaac gacaagcccg cagaggtgta ttgcccttca tggtagttcg
    45901 cgctgaggtc aacctgcgcc agcgaaccat catggctgta atagccgtca acgctggtgt
    45961 gtttgtcact ggtgccgacg ttcaactgat agtgagtcgc gtcatcgaca cggctgaaat
    46021 aaccgacctg actgctgtcc gtcccactgc catagttgcc gttatagcta acggtactgt
    46081 tgtcgcccca cggcatactg agcgaaatgt acatgccctt atccgcccgg ttgtcatact
    46141 cgtagcggta gccagtcagg gaaacgctca tattacgaat gctgcccata ttgaaatagt
    46201 gggagagcat gatgttgtag tttgtctgct cctcacgatc ccagtaagta tggcgggtat
    46261 agttgagata aaccgaaaca cccgcatcgc ggaagttctg gttataagtg gcggtgtaca
    46321 tctctttgtc gttgcccgtg cgtaccattc cgctgtcact ggcatccaga tactcgctca
    46381 tggtcataaa gttctcttcc gagaagcgat atccagcaaa agtaacgcgg ctgttgagct
    46441 ggtcaaagtc tttggaataa ctcacacgga aggagttacc gtccagcgaa cctttgccat
    46501 aagcggtgtc tttatccagt ttggtgtgcg agtgagtaac atcaaacgcg accgcgccga
    46561 acgtagacaa atcgcgacca acgccaagtg cggcagattg gtagttttca tcccccagcg
    46621 cgccaccata gagcgaccaa ccattagcga tcccccagga agcttcagca ccagagaaaa
    46681 attcgccttc aacatgatgc ccccactctt gcggacgacc catcatgatc ttatagcgca
    46741 cctgacctgg acgagtgagg tatggcatcg aggcggtgct gatgtcatat tcctgcacct
    46801 ggccgttctg ttcttcaatg cgaatatgca acgtaccgga gacagaatca ccaagatcct
    46861 gaatacgaaa cggtccagcc ggaacctgcg tttcgtaaat cacacgcccc atctggctga
    46921 cggtcacttt tgctgtggtg tgtgccacgc cggaaatgtc tggcgcgtag ccgcgcaggt
    46981 tgggaggcaa catttgatcg tcagtgctga cactgccacc aacataatta aaaccgtcga
    47041 aaatatcgga attgaggtaa tcttcgccca gcgccagttt ggcttttaat gacggtaacg
    47101 cccgccaggc ataatagcga ctccactccc attttttttg tgtgtcatcg ccgctaaatt
    47161 cctcgtcatc atcattactg cgggtatgtt gatagttggt ctgccagtca gcgcgcatac
    47221 gccatggccc caggttaacc ccgaccgtcc cgttgccgct gatctcgtta ctatcatcac
    47281 cgccattttc ttcgtgccgg gtttgtgcgt tgatgctgta gtccgcgacg atcccggaga
    47341 tgccgtcatc ccaacgtgaa ggcggatccc agtcgggata agtatattcg aggtaagcct
    47401 gtggcagtga aatgactaat gcggactggc ttaaatcagc cttaatttcc atgccttcca
    47461 gttgaccgga tttcaggcat ttagcatcgt ggctccattg cagatttttc gccacgtctt
    47521 cttttaaacc aaactgcgct accagttccg gtgtcagaca agcatagctt ttgctcgcgt
    47581 catcttcacc agcataccag taaatatcgt actcttccgc caatggctgt ttatttagtt
    47641 gaacctgtaa attgtatttt ccgggctcaa catatccctg gctggaaaaa cgcttcagat
    47701 caatttttgt gtcgcctttt aattccagaa aacgggaatc aaactgaatg tcgtcttcag
    47761 cccagacact gctataactc cctgacattg ccagcgcaat gcaccacgga agaatccgta
    47821 atcgaaaaag agaatggtca ggcatacacg tttctatccg tttttattct tgttcggtca
    47881 aaatccattt aaatccccca tagtagtgtc gctatggggg tatgccttac aggtaagtaa
    47941 tctggaaggt ggtatttgct tcgaaattac ccagatctgg cgcatcagct gcgccgacca
    48001 gccaggcttt aaagttcagt gtctgcttcg ctttgccttt attggtagaa gtgtctttca
    48061 ctattttctg gtcaataccc atgccgcttt tgtaagaggt acccagagag tcaccgatcg
    48121 ccaggctaac attgttaaat gccgcaccgg tatcggtatt gaaaatggtg taataattgc
    48181 cgctatttgc agaagaaacg gtaccagtaa aggtagtagt cattgtttcc tgagtatcaa
    48241 atacgcaatc ttgcagacga atctggaaac ttttcggcgt ggtggtgccg ttatttttca
    48301 gtttttcggc accgatatca cccatatcaa cttccagttt gtgatcgtcc ggaacgatag
    48361 aacacactga gtcttcaatg gtgccgtaaa aacgtgcagt accgttgtta cccgcagcga
    48421 ttgttgccgt agaggtga
//
LOCUS       DAJOWB010000022        47438 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJOWB010000022 DAJOWB010000000
VERSION     DAJOWB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.022"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            84..1409
                     /locus_tag="OHO85_001415"
     CDS             84..1409
                     /locus_tag="OHO85_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HCQ2670816.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLLVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREERDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKRLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGKRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            1518..1754
                     /locus_tag="OHO85_001416"
     CDS             1518..1754
                     /locus_tag="OHO85_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCQ2670817.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKTILKHISVSSVSASPTVLE
                     DAIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            1766..2359
                     /gene="rclC"
                     /locus_tag="OHO85_001417"
     CDS             1766..2359
                     /gene="rclC"
                     /locus_tag="OHO85_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HCQ2670818.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(2519..2647)
                     /locus_tag="OHO85_001418"
                     /pseudo
     CDS             complement(2519..2647)
                     /locus_tag="OHO85_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            2950..3801
                     /locus_tag="OHO85_001419"
     CDS             2950..3801
                     /locus_tag="OHO85_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2670819.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLINFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCEYADMPKEWTTTRGLY
                     ASFRYEGNWENYPDWVRNIYLIELPARGLARVNGSDIERFYYNEDFVEKDGNDVVCEI
                     FIPVRPV"
     gene            complement(3941..8197)
                     /gene="fdeC"
                     /locus_tag="OHO85_001420"
                     /pseudo
     CDS             complement(3941..8197)
                     /gene="fdeC"
                     /locus_tag="OHO85_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001635811.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
     gene            9312..9413
                     /locus_tag="OHO85_001421"
     CDS             9312..9413
                     /locus_tag="OHO85_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670820.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            9777..10040
                     /locus_tag="OHO85_001422"
     CDS             9777..10040
                     /locus_tag="OHO85_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCQ2670821.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            10040..10180
                     /gene="ykgO"
                     /locus_tag="OHO85_001423"
     CDS             10040..10180
                     /gene="ykgO"
                     /locus_tag="OHO85_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HCQ2670822.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(10215..10442)
                     /locus_tag="OHO85_001424"
     CDS             complement(10215..10442)
                     /locus_tag="OHO85_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670823.1"
                     /translation="MQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            11265..11807
                     /gene="ecpR"
                     /locus_tag="OHO85_001425"
                     /gene_synonym="matA"
     CDS             11265..11807
                     /gene="ecpR"
                     /locus_tag="OHO85_001425"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HCQ2670824.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLSTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRLLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            11882..12469
                     /gene="ecpA"
                     /locus_tag="OHO85_001426"
                     /gene_synonym="matB"
     CDS             11882..12469
                     /gene="ecpA"
                     /locus_tag="OHO85_001426"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HCQ2670825.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFTII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            12527..13195
                     /gene="ecpB"
                     /locus_tag="OHO85_001427"
                     /gene_synonym="yagY"
     CDS             12527..13195
                     /gene="ecpB"
                     /locus_tag="OHO85_001427"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HCQ2670826.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKTANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            13221..15746
                     /gene="ecpC"
                     /locus_tag="OHO85_001428"
                     /gene_synonym="yagX"
     CDS             13221..15746
                     /gene="ecpC"
                     /locus_tag="OHO85_001428"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HCQ2670827.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            15736..17379
                     /gene="ecpD"
                     /locus_tag="OHO85_001429"
     CDS             15736..17379
                     /gene="ecpD"
                     /locus_tag="OHO85_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HCQ2670828.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            17348..18058
                     /gene="ecpE"
                     /locus_tag="OHO85_001430"
     CDS             17348..18058
                     /gene="ecpE"
                     /locus_tag="OHO85_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HCQ2670829.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            18371..18700
                     /locus_tag="OHO85_001431"
     CDS             18371..18700
                     /locus_tag="OHO85_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCQ2670830.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(18948..19562)
                     /locus_tag="OHO85_001432"
     CDS             complement(18948..19562)
                     /locus_tag="OHO85_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HCQ2670831.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            19980..20669
                     /gene="paoA"
                     /locus_tag="OHO85_001433"
     CDS             19980..20669
                     /gene="paoA"
                     /locus_tag="OHO85_001433"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit"
                     /protein_id="HCQ2670832.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            20666..21622
                     /gene="paoB"
                     /locus_tag="OHO85_001434"
     CDS             20666..21622
                     /gene="paoB"
                     /locus_tag="OHO85_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HCQ2670833.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFAQVSVAAIIHPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            21666..23831
                     /gene="paoC"
                     /locus_tag="OHO85_001435"
     CDS             21666..23831
                     /gene="paoC"
                     /locus_tag="OHO85_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015696197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HCQ2670834.1"
                     /translation="MDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEEAPNAAYGYIVG
                     SAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGGPTIEHYHQAIA
                     LVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEGTPDKNVGDFDGAFSS
                     AAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRTDLAKTLKVPVE
                     NVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPTIPNNTTHRPATLQ
                     HLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHTGLRLATLDLPE
                     GNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADPTRRFSRRQLIE
                     CLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGARVHLEPNGTVTV
                     ETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGGQWGANTSTSGV
                     YAACVKLREMIASAVGFDPEQSQFADGKITNGTRSAMLHEATAGGRLIAEESIEFGTL
                     SKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARSQVIGAMTMGMG
                     AALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPISSPMKAKGVGEL
                     GLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            23841..24797
                     /gene="paoD"
                     /locus_tag="OHO85_001436"
     CDS             23841..24797
                     /gene="paoD"
                     /locus_tag="OHO85_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HCQ2670835.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPTSASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            24848..25186
                     /locus_tag="OHO85_001437"
                     /pseudo
     CDS             24848..25186
                     /locus_tag="OHO85_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308332.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(25313..25388)
                     /locus_tag="OHO85_001438"
     tRNA            complement(25313..25388)
                     /locus_tag="OHO85_001438"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25353..25355),aa:Thr,seq:cgt)
     gene            complement(25503..26756)
                     /gene="proA"
                     /locus_tag="OHO85_001439"
     CDS             complement(25503..26756)
                     /gene="proA"
                     /locus_tag="OHO85_001439"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCQ2670836.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPVGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MEESGVALHADAAALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(26768..27871)
                     /gene="proB"
                     /locus_tag="OHO85_001440"
     CDS             complement(26768..27871)
                     /gene="proB"
                     /locus_tag="OHO85_001440"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCQ2670837.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            28159..29214
                     /gene="phoE"
                     /locus_tag="OHO85_001441"
     CDS             28159..29214
                     /gene="phoE"
                     /locus_tag="OHO85_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCQ2670838.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(29253..29654)
                     /gene="crl"
                     /locus_tag="OHO85_001442"
     CDS             complement(29253..29654)
                     /gene="crl"
                     /locus_tag="OHO85_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCQ2670839.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(29712..30956)
                     /gene="frsA"
                     /locus_tag="OHO85_001443"
     CDS             complement(29712..30956)
                     /gene="frsA"
                     /locus_tag="OHO85_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCQ2670840.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(31048..31506)
                     /gene="gpt"
                     /locus_tag="OHO85_001444"
     CDS             complement(31048..31506)
                     /gene="gpt"
                     /locus_tag="OHO85_001444"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCQ2670841.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDNYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            31767..33224
                     /gene="pepD"
                     /locus_tag="OHO85_001445"
     CDS             31767..33224
                     /gene="pepD"
                     /locus_tag="OHO85_001445"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCQ2670842.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQDILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIKSVGHYWTLLTELLKEIPAK"
     gene            complement(33281..33633)
                     /gene="prfH"
                     /locus_tag="OHO85_001446"
                     /pseudo
     CDS             complement(33281..33633)
                     /gene="prfH"
                     /locus_tag="OHO85_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="similar to release factor 1 and 2; frameshifted;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(33629..33835)
                     /locus_tag="OHO85_001447"
                     /pseudo
     CDS             complement(33629..33835)
                     /locus_tag="OHO85_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
     gene            complement(34154..34606)
                     /locus_tag="OHO85_001448"
     CDS             complement(34154..34606)
                     /locus_tag="OHO85_001448"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCQ2670843.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVMMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFISRIERHIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQIVKQQHVECRGAWFTNFYMRYKPQH"
     gene            complement(34603..35658)
                     /gene="dinB"
                     /locus_tag="OHO85_001449"
     CDS             complement(34603..35658)
                     /gene="dinB"
                     /locus_tag="OHO85_001449"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCQ2670844.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELHLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(35729..36514)
                     /gene="lafU"
                     /locus_tag="OHO85_001450"
     CDS             complement(35729..36514)
                     /gene="lafU"
                     /locus_tag="OHO85_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCQ2670845.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFSGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIIIGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            36486..38198
                     /locus_tag="OHO85_001451"
                     /pseudo
     CDS             36486..38198
                     /locus_tag="OHO85_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(38516..39013)
                     /gene="rayT"
                     /locus_tag="OHO85_001452"
     CDS             complement(38516..39013)
                     /gene="rayT"
                     /locus_tag="OHO85_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCQ2670846.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRHAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(39189..39938)
                     /locus_tag="OHO85_001453"
     CDS             complement(39189..39938)
                     /locus_tag="OHO85_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCQ2670847.1"
                     /translation="MSSFLLGRFLHPGVFSLCVLLPLFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GENIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            40239..40979
                     /locus_tag="OHO85_001454"
     CDS             40239..40979
                     /locus_tag="OHO85_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCQ2670848.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(40950..41717)
                     /locus_tag="OHO85_001455"
     CDS             complement(40950..41717)
                     /locus_tag="OHO85_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCQ2670849.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(41923..42501)
                     /gene="lpcA"
                     /locus_tag="OHO85_001456"
     CDS             complement(41923..42501)
                     /gene="lpcA"
                     /locus_tag="OHO85_001456"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCQ2670850.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            42741..45185
                     /gene="fadE"
                     /locus_tag="OHO85_001457"
     CDS             42741..45185
                     /gene="fadE"
                     /locus_tag="OHO85_001457"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCQ2670851.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGTPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLCSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(45228..45674)
                     /gene="ivy"
                     /locus_tag="OHO85_001458"
     CDS             complement(45228..45674)
                     /gene="ivy"
                     /locus_tag="OHO85_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCQ2670852.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            45855..46625
                     /gene="yafV"
                     /locus_tag="OHO85_001459"
     CDS             45855..46625
                     /gene="yafV"
                     /locus_tag="OHO85_001459"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCQ2670853.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQNDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWQDADEFRLR"
     gene            complement(46667..>47438)
                     /locus_tag="OHO85_001460"
     CDS             complement(46667..>47438)
                     /locus_tag="OHO85_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCQ2670854.1"
                     /translation="RGAIHVISAFSTMHSLVIGQIRTDEKSNEITAIPELLNMLDIKG
                     KIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYA
                     MSEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVR
                     YYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIA
                     INILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
BASE COUNT        11759 a        12191 c        11885 g        11603 t
ORIGIN      
        1 tcaagcgttg tgaagaaatg ttatttgctc ttttgcgtct atagtcatga tgtcaaatga
       61 acgcgtttcg acaggaaatc atcatgaata aatatcaggc agtgattatt ggttttggca
      121 aggctggaaa aacattagcc gtcacgctgg caaaagcagg ttggcgagtg gctctcatcg
      181 aacaatcaaa tgcaatgtat ggcgggacct gtattaatat cggctgcatc ccaaccaaaa
      241 cattggttca tgacgcacag cagcacacag attttgtccg tgccatacag cgtaaaaatg
      301 aagtggttaa ttttttacgt aataagaatt ttcataatct tgcggatatg cccaatatcg
      361 acgtgatcga cggccaggcg gagtttatca ataatcatag cctgcttgtt catcggcctg
      421 agggaaatct ggaaattcat ggcgagaaaa tttttattaa taccggtgca caaaccgtgg
      481 ttccgccaat tcctggaatt accaccacgc caggagtata tgacagcacc ggattactta
      541 atctaaaaga attgcctggg catttaggta ttttgggcgg cggatatatt ggcgttgagt
      601 tcgcctctat gttcgctaat tttggcagca aggtaaccat tttagaagca gcttcactgt
      661 ttttgcctcg ggaagaacgg gatattgctg ataatatcgc gacgatttta cgcgatcagg
      721 gcgtcgatat tatcctcaat gcccatgtgg agcgaatcag tcaccatgaa aatcaagtgc
      781 aagtgcatag cgagcacgcc caactggcgg tggatgcact gttaatagct tccggtcgtc
      841 aaccggctac cgcttcgtta catccagaaa atgccggtat cgcagtaaac gagcgcgggg
      901 caattgtcgt tgacaagcga ttacatacca ccgcagacaa tatttgggcg atgggagatg
      961 ttaccggcgg gctgcaattt acttacatat cactggatga ttaccgcatt gtacgtgatg
     1021 agttactggg tgaaggcaaa cgtagtactg atgatcggaa aaatgtgcct tattccgtat
     1081 ttatgacacc gcccctgtcc agggttggta tgacagaaga acaagccaga gagagtggtg
     1141 ctgatattca ggtggtgaca ttgcctgtag ctgcaattcc gcgcgccaga gtgatgaacg
     1201 atacccgtgg ggtattaaaa gcgattgttg ataataaaac ccaacgtata ttaggggcat
     1261 cactgctgtg tgttgactcc cacgagatga ttaatatagt gaaaatggtg atggatgccg
     1321 ggctgcctta tagcatatta cgcgatcaga tatttactca tccgtcgatg agcgaatcac
     1381 tcaatgatct attttcatta gtcaaataaa ctcaaaatca gacgccagaa caaatattct
     1441 ggcgtctcag agaaaagaat cttattaatt ccctgttact ctataacttc ttaatcactt
     1501 cattgatggt attttatatg tttaaaaaat ccgttttatt tgcaacacta ttatctggcg
     1561 ttatggcatt ttccaccaat gcagatgata aaacaattct gaaacatatc agcgtctcgt
     1621 cagtatcagc atcaccgaca gttctggagg atgccattgc tgatatagcc agaaaatata
     1681 atgcttcatc ctggaaagtc acatcgatgc gaattgataa taattcaacc gcaacagcag
     1741 tattgtataa ataaggatgt tcacaatgga aaaatacctg cacctgttaa gtcggggaga
     1801 taaaattggc ctgacattga ttcgtctgag tattgcaatt gtttttatgt ggattgggtt
     1861 attaaagttt gtcccttacg aggcagacag cattacacca ttcgtcgcaa acagtccact
     1921 aatgtcgttc ttttatgaac acccggaaga ctataaacag tatctgactc acgaaggcga
     1981 atacaaacca gaagcaaggg catggcaatc ggccaataat acctatggtt tttccaacgg
     2041 tcttggcgtc gtggaggtga ttattgctct gctggttttg gctaatcctg tcaatcgctg
     2101 gttaggttta ttgggagggc tgatggcatt taccacaccg ttggtaacac tctcattttt
     2161 aatcaccacc ccggaggcat gggtacccgc attgggtgac gctcatcatg gtttccctta
     2221 tttatccggt gctggtcgcc tggtattgaa agatactctg atgctggcag gtgcagtcat
     2281 gataatggca gattcggcgc gggaaattct taaacaacgc agtaatgaat ccagttcaac
     2341 gttaaaaact gaatattgat aatccgaaca ctgtctgttg ttcacttttt caggcgtcag
     2401 gccccctttc tgatgatgaa gcctgacgcc atattaccgg agatatgaca gagtgaagat
     2461 caatatcact cccctgttaa aaggtttttg tcgtcttagg taaaatacat gattaatatc
     2521 aatcatgtat ttttacagca agacaaccac gaacaaattc aggattaaaa tgttttaccg
     2581 actccccaac ataacccaaa tcaagcgaac taatttgtgc catttcttta tctgtcagtg
     2641 agaaatcgat ttttgatggg catctgcaat actctgaagc agactatacg gaaaattcca
     2701 ctgtcagtcc ctccattagg catgaacaat gagtctacgt taaaacgtaa cctcaaagta
     2761 gtatgtggat tttgatatca cttatgcaaa aaattcatta ataatgtagg actgaaacct
     2821 ctctattttc ggggacaacg aagcagacgc taccagtgct tttgccttcg cccttgctat
     2881 ttttgataca cttagggccc agggtataac gaaaatgtgc gatatgacaa agaattaacg
     2941 gagaatgaga tgatcaggca gaagattcta cagcagctcc tggagtggat tgagtgcaat
     3001 cttgagcacc ctatttcaat cgaagatatc gcacagaaat ctggctacag cagacgcaac
     3061 atccagcttc tgttccgaaa tttcatgcat gtgcctttgg gagaatacat tcgcaaacga
     3121 aggctttgtc gtgccgccat tcttgtccgg ctcaccgcga aatctatgct tgatattgca
     3181 ctctctttgc attttgattc acagcagtca ttcagccgcg aatttaaaaa gttattcggc
     3241 tgctctcccc gtgaataccg ccaccgtgat tattgggatc tcgcaaatat cttcccttct
     3301 tttttaatac gtcaacagca aaagacggag tgtagattaa tcaactttcc tgagacacct
     3361 atttttggca actcatttaa atatgacatt gaagtgtcga ataaatcacc ggatgaagaa
     3421 gtcaaactac gacgtcatca tttagccaga tgtatgaaga attttaagac ggatatctat
     3481 ttcgtttcca cgtttgaacc gtcaacaaaa tcggtcgatt tgctcacggt tgaaactttt
     3541 gctggtacgg tatgtgaata tgctgacatg ccaaaagagt ggacaacgac ccgaggactt
     3601 tatgcctctt tccgttatga aggaaactgg gaaaattatc ctgactgggt gcgtaacatc
     3661 tatctgatag agttacctgc cagggggtta gccagagtga acggcagcga tattgagcgc
     3721 ttttattaca atgaagattt cgtagaaaag gatggcaatg atgttgtttg cgaaattttt
     3781 attcccgttc gtccggttta gttggtcact atctcttatt gagttttatc cttgcctgat
     3841 actatttaat cagtatcagg caaggtattc agtgaataat ggcgttgaat atttcaacac
     3901 cattattctt tattagatcg taactttcat actattcaaa ttatggcatc tcctcctcgc
     3961 cattttcagt ctctgcagca acggcgttaa ttgtcacctt cacaggcgtc atttcgtacc
     4021 cgccatacgt cagtgcgttg aatgtgaacg tatagacacc tggcttgctg gtactaaatg
     4081 tacgcgtgat tttcccacct gagaaatggt cagccttcga cggcaggaat tgataatcct
     4141 tctccgtcac tccttccgga gcctcaatgt cgacccacat acttcctcct acgggattga
     4201 ctcccgccat cagggtaacc gtcattgttg cagtagtctt cccgtccgcc accagagaat
     4261 ctacacgttc tggagatgcg gcgagcaccg cgtttttatc atccgcgacg aatttagatt
     4321 cagcagtttt cgtcgatacc tgaccgttgg cctgttccac tttcgccgtg agtgtatatg
     4381 tcgctgcgat agtggtagag ccggtgaaaa cagcctgtcc ttgctcatta gttttcgccg
     4441 tccctttagg atccagaact aaattttccg agctggcagt cagcgtcacg tcgctatcct
     4501 tcaacaggtt gttattggca tcagtcaccg taactttata cgtctgtttg gttttgccat
     4561 ccgccactgc acggttgcca atcacctcaa tactggagat ggtcgccgta gtcttatcgg
     4621 ccacaaacgt tacagactgc gcattcgaag gctggtcatt tcctgccgac gccgtgatct
     4681 catacgttcc ggcagtcacg gagaccactt tcagttccgc tttaccctcc ttgtcggcgt
     4741 tcaccatgat attaccgtct gcaagcggtt tgacgccccg aggcagatta aagacgacca
     4801 cagcgccagg cagaaggttg ccgtattgat ccttaaccgt tgccgtcagc gtaaaggcat
     4861 cattgtcgtt tgccactttt ggcgtgctgc catcaacctc cagggtcgcc tgaccggtac
     4921 tgaaatccgc cttgaatttc accgtaaccg tcttctgagc attattcact gaggccgtga
     4981 tgctgtgtta ccccgcaacc gttgacataa gctcaatgtc cactttccct gccgcattgg
     5041 ttgttactgt attccccgtc ttgctggtca caccctgcgg taaagtcagc gtaacttctt
     5101 gcccctgcaa cgggttacca tagctgtcca cgacggtcag ggttatctgg ttagcagact
     5161 gtccattagc cagttggtta ttaaccttca ctgtcatatc acgaatctca gccttagatg
     5221 cgtcacctgc aacgttcagc accagtggct gagcaacggc attttggccg ttcactcgcg
     5281 gcatcacaga caactgaccc gcggcagatc ccagcgttaa ggtcgccacg tagaccccat
     5341 tacctttctc tgtccagttt cctgctgaag gacgctcact ccccgtgctg gcggcaccgc
     5401 taaacactgg tgcatctggc ttcagcccgg tgaccgggtt cccgtacgca tccttcacgg
     5461 tgaaggtgag ttccgtcgtc attttgacgg tcggagcctt attgtccgca acaagcgtag
     5521 agttcgctga tgacatctct ccggcgatga ccgtcaactg cgcggcttcg gtggctgctg
     5581 gctggccgtt gaagagcggc atgacacgaa gctcgcccgt ctttccgcct gtagttaacg
     5641 tagcaacata ggaaccgtca cctttttcgg tccaactgga aacggtcgcc ccttcagagg
     5701 cggtccccgt caaacttgcc gacaacgaaa gaccactgat agtgttgcca tgtgcatctt
     5761 ttgcaataag cgtcacggtt gtgctttcgc cggcttttac gtgatcttct gcgacagaga
     5821 ctttcgactg gtttgaagat aacgcatcag ccaccacggt tacttttgcc gcatttgctg
     5881 ccgcatcctg tccatttagc ttcggcataa cttctaattc acccgccgta gagccgagag
     5941 taatctgcgc agtccacgtc ccatcatcat tatttgtcca gccagatgcc gtagaaccta
     6001 cagcagcggc acccgctaat gacggcgctt ccggcgtgag gctggtcaca gggttgtcat
     6061 aggcatcttt tgccgtccag attgccgtaa ctgtaccgcc aaccaccggt ttatcaggat
     6121 tcagggtgat ggacgaatgt gctgcatcaa gcggaccggc aacaaacttc agcgtttgtt
     6181 gtaatgtgcc cagctgatac tgctcgctga aagcacgcac aacaacgttt ccggcacggg
     6241 tcgaagaaac cgtggcgctg taaacgcctg gttttatttc cgaaatggca ccaacggtta
     6301 caccattagt gtcttgcgga acaaatcgca agcggctggc ttctcccgtc accggattac
     6361 cctcggagtc caccgcagtc aacgtcaacg tataggcttg ctgaccatca gcaaccacat
     6421 cacctgacgg ctcgttagcg ctcagggtgg agtttgccac atccatcatc gtggcccgca
     6481 gttctgcagt gacggttttg ctcatgccat caacgctaac agtaatcgtt gcctcacctg
     6541 actgcgttcc ggtagtaaag acagactgat acacccctgc ttcagtttcg gtgaactcac
     6601 ccagtgttgg ctttgcctgt gatttagtgg ccttcaggga acgagtcaca atatttccag
     6661 ccggtttgaa ggctagttca gtcttgatct gatctttcat gcccgtgact ggctgcccct
     6721 cgacgtcgcg cagagacagc accagcggtc tttgctcatt accgttagca agcatttgaa
     6781 tacggctctg accgtcaagc gttaacgccg tgcgatcggc gctcatacct gctccggtaa
     6841 tgaccacctc tgtctgcacg cgttttgagg cattgccttt gttatcgtag gcaaccgcag
     6901 aaatcgcata ataattgtct ttgcctggac gataagccgg gagcgttact tgccactgac
     6961 taccctgacc ggtaattttg ccaccctcag ccagtaatga cggcgcttcc cactgcacat
     7021 ttttcagtcc gtgagttgct ttgctgacca taagccccag ggaaagtgtc tgaccaccct
     7081 taccttcaat acgttcaggc agagcaatac ggatcacttc agatttgcgg tactcaagaa
     7141 cgatgttgtt attacgctca accaggtcat agcggctgcc tgccagtacc cgacgctcgc
     7201 gaatgctgtc tgtatcgagt tgtttcgcca gaggttcgcc aatccgataa ttaacttcca
     7261 ggccaaagcg agtgtcattc tcaccactct tgccctgctt atgcccggcg ctcagtgtca
     7321 gaagaggcac tggcgtatag gtcacctcgg cagaaatagc atgcgggtct ttctggcgct
     7381 tatctttacc aaacagcccg acttcatcgc cataatactg ttcatacatc aggcttgcgc
     7441 caagctgcgg ccaggcgggt aaatagccct cagcacgaat atcccagcca ttcgccgggc
     7501 gttcctgata atcctcaaca tccggcgatt ttttccagcc agaagcccgg atataaccat
     7561 tggcgctcag tttcagataa tcgcgccagt attccgcacc aacaccaatg cgggtatgac
     7621 tacgggataa atcatggtcg ataaaggtgt tcacccccgc catccagtca tttcctgaaa
     7681 aatgacgcca gccaaaacca atatttgact gagtacgatc gtcggtacga tgtattgctc
     7741 cctgagtgaa caacatattt gttggcgtat cataaatcgg ataaagcatt tccagcgaag
     7801 aatccttcag cgagaaatct ttatcgacat tcagtttgac gcgcgcagta ccatatttcc
     7861 cgagccactc ctgtatttcc tggttagctt tagctgtggc cattccggta ataaagttac
     7921 gtgtcgcatc gctatctggc tgactgctta aaaatgtccc ggcatttgcg gcaaacgacg
     7981 cgacattttt ctccacgtta ttatcagcag ttaccgtagt atttcccatg ctcaaccgtg
     8041 gctgaaccgc atgctgcgca cgtgccgcca ttactggggt aaaggtgaca gcgagtggaa
     8101 aaagaacctg aacagagata tttgcccacg ccacgcagcg ggccagaact gaataacgaa
     8161 atcgtggttg tttatgacct gttttataat gtgacattga gataatttcc tgatgaacga
     8221 attaacgaac ataggcagac acaaataatg gcgtctgctt gtcagaaaaa ccggggattt
     8281 cccgatttcc ttttccgcag aaaaggaaat acctttatgt aagaaagtgc accgccacct
     8341 aatatatcca gtgcctgtag ccactcatca ggtggttggg cgcgggatta tatttttatt
     8401 tatgggggaa aatttagctt tttgtgcaag tcattaaata gatacctaag ttatatttct
     8461 tactctgcgg acagtgaata attcactatt cagagtgaat aacaatgacc tgcctttaat
     8521 taaaactatt ggaaatacgt cgaaataggt atatatgcca ggataaacca atatattttt
     8581 tatggatatt cattttcctt catcggactg taaattgcgg agatatcgaa cttgcactac
     8641 ccatcgcaac actcaaacag atttatgaat ttcgttcata acccctgaca aattctgccg
     8701 tctaatcgag cttttccctt tacgttatgc ttctcatgaa cgataacaca acttgttcat
     8761 gaattaacca ttccggataa actatgggat caaattcagg catcgaaagg aactggctgg
     8821 tttccgttaa tggattaaat atctattatt ctcccgagag taaaactatc aaatcaatga
     8881 gtaaccgata cagcctcaca aattctttca gataattaac ccgtcactgc ggcacgttct
     8941 ctcataaggc gtgcctgcag tgtaatccag tatgtatccg gcatagttga cggtttaata
     9001 gaactcaatt cctctcccag gtcaccaaca acgatatctt gtataacacc ccaggagata
     9061 cgacattatg atgagatgcg atatgctcct cagcaccaat caacgcccgg actgggccag
     9121 tcttgagatc agactcattc ccggaactga cttcaggcga catatatcaa tgacgttggt
     9181 gcaataaaag atagttgatg taatacatct attcaaattt gtgatacatg tcaaaaaatt
     9241 ggttgaccaa actcgttatt tatataaggg cacttacgaa gtgcactctt ttttaaagcg
     9301 aggaagtacc aatgaaagag aataaagtac agcaaatcag tcataaactg attaatatcg
     9361 ttgtttttgt cgcaattgta gaatacgcct atttatttct ccatttctat taataacgga
     9421 aataaactgt tcacttcagt gatatttaaa atatgcatcc tctccctttt ttgtaagtaa
     9481 ttattatatc cgtgggagag gaatacacgc tgtcaggtaa tcaatcatac tgcgataaat
     9541 catcggccag taaagtggag ataacctcca ttctcgaaaa atccatactc tcagcaaaac
     9601 catcatcaat cactcatcca ggcgtttatg ggagcgtcac caatggctgc taacaatgcc
     9661 agagttcccc gttgcgaaaa ttccacatcc acaaagagtc acagggattg agtgttgaaa
     9721 tgatccggat gagcatgtat ctttatggtt atgttataac ataacaggta aaaatgatga
     9781 agcccaatat ccatcctgag tatcgtactg tggtgttcca cgacaccagt gttgatgagt
     9841 actttaaaat cggctcgact atcaaaacag accgtgagat tgagctggat ggcgtaacgt
     9901 atccatacgt gacaattgat gtctcttcta aatcgcaccc gttctataca gggaagctga
     9961 gaacagtggc atcagaagga aatgttgcac gattcaccca acgttttggt cgttttgtta
    10021 gcacgaaaaa gggggcgtga tgaaagttct taactctctg cgtaccgcaa aagaacgcca
    10081 tccagactgt cagattgtga agcgaaaagg acggctatat gtgatttgta aatctaatcc
    10141 acgttttaag gccgttcagg gtcgtaagaa aaaacgttga ttcaaaattc gacggattaa
    10201 cgatatttgt ctgattaata atcagatcgg attaatgttg gtgtgtttat aacaccaaca
    10261 ttaattttcc tggggatata ttcttcctgt tcatttgagg ccaactgcct gacgtttctc
    10321 tccgaatatt ccattatctt aatgttgact tgttgaccag cttcgcccct gtatgctggc
    10381 atcaaccctc ttttagactg aacacgccac tcagtctcct ccctttgcgg cgcagcctgc
    10441 attttcactc aaactgttaa gatgataaat gtggtaaatc tgttggtact aacataaaaa
    10501 cgtttacgcc acaggaacag tctgatccac cggtaacccc gtcgccgacg ttcgagtgcc
    10561 agttagagta acgcgcacag ataactgaat gcagtgccct gacaaaaagg ccatcgttcc
    10621 tgtgacagct ggcagccttc gtttaacttc acttaatctg gctcttgggg gcttaccgaa
    10681 cagatgacgt acatacgccc gttcaatttt ccattactta ttggaatgaa cacctgtaac
    10741 cattttgtgc ggcatgttaa tccattaaaa tatcttactg attggcaaat catcttcaat
    10801 gacagctcat catagtttta tattctatcc cttaccctta aaacttgttt ttttactagt
    10861 ccatcacaca gcgcattaag actattccta acacttcagg gcaaagttcc tgaccaatat
    10921 aaaatgcaag taagaattga acgttatatt gccaataacc ttatgaaacc aaatgtcttt
    10981 ttcttcttat caaaaaagca atattttcag tttttctaaa tattgactta accattgaat
    11041 tccttttccg ttcacatatt gacactcatc gggaaaaaaa cataaattta agcccaatcg
    11101 aaaataatta aacttaatct cgtttaacct ttattgatat gtactacgta tcttatttac
    11161 ttccggttta ctaaggaaac tgaatgcacc tgtaaaaatt acaggtttgg aaagtagtga
    11221 catggcaaag tgattacagt agggactatg aggttaaaaa ccatatggaa tgtcaaaacc
    11281 gttctgataa atacatctgg tctccccatg acgcctactt ctataaagga ctatctgaac
    11341 tgattgtgga tatcgacaga ttaatttatc tatcgttgga gaaaattaga aaagatttcg
    11401 tgtttatcaa tctcagtacg gattctttat ctgaatttat aaaccgtgat aatgaatggt
    11461 tatccgcggt aaaggggaaa caggtcgtat tgattgcggc cagaaagtca gaagccttag
    11521 caaattattg gtattacaat agcaatatta ggggcgtggt atacgctgga ctgagtcgtg
    11581 atattagaaa agaactggcc tatgtgatta atggcaggct cctgagaaaa gatattaaga
    11641 aagataaaat cacggaccgg gaaatggaaa ttatccgcat gacggcccag ggaatgcaac
    11701 ctaaatcgat tgccagaatt gaaaattgta gtgtgaagac agtgtatacc catcggcgta
    11761 atgctgaggc caagctgtac tcaaaaatat ataagttggt tcagtaaact ccaggcaagt
    11821 tagttttaaa aaatgactca ctgggacatc acgtcctcaa ttcaactcgg gaagaaatgc
    11881 aatgaaaaaa aaggttctgg caatagctct ggtaacggtg tttaccggca tgggtgtggc
    11941 gcaggctgct gacgtaacag ctcaggctgt agcgacctgg tcggcaacag ccaaaaaaga
    12001 caccaccagt aagctggttg tgacgccact cggtagcctg gcgttccagt atgccgaagg
    12061 cattaaaggt tttaactcac agaaaggtct atttgacgtg gctatcgagg gtgactcaac
    12121 ggctaccgcc tttaaactga cctcacgtct tatcaccaac acattaaccc agttggatac
    12181 ctcaggttcc acactgaatg tgggcgtgga ttataacggc gcggcagtcg aaaaaactgg
    12241 cgataccgtg atgatcgata ccgccaacgg cgtactgggc ggcaacctta gcccgctggc
    12301 taacggttac aatgccagca atcgtaccac cgcacaggat ggtttcacct tcaccatcat
    12361 cagcggcacc accaatggta ccaccgcagt aaccgattac agcactctac cggaaggcat
    12421 ctggagcggc gacgttagcg tacagttcga cgcgacctgg accagttaat ctctctgatg
    12481 taccagcagg ggtagccccc ctgctttatt cctggacgga attgttatga aaaagcacct
    12541 tctgcctctc gctctgctgt tttccggaat atctccggcc caggcgctgg atgtcggcga
    12601 tatatcatcg tttatgaaca gtgacagcag cacgctgagc aaaacgatca aaaacagtac
    12661 cgacagtggt cgccttatca atatccgtct cgaacggctc tcttcaccgc ttgacgacgg
    12721 gcaggttatc tcaatggaca agccggatga gttgctactc actcccgcca gcttgctgct
    12781 acccgcccaa gccagcgaag tgatccgctt cttctataag ggacccgcag atgaaaaaga
    12841 gcgctactac cgcattgtct ggtttgatca ggccctcagt gatgcacagc gcgataatgc
    12901 caaccgcagc gctgtggcca ctgcttccgc ccgcatcggc accattctgg tcgtcgcacc
    12961 ccgccaggca aactaccact ttcagtacgc caacggctcc ctgacaaata caggaaatgc
    13021 gacgctgcgg atcctcgcct acggcccctg cctgaaaacc gccaatggta aagagtgtaa
    13081 agagaattac tacctaatgc cgggcaagtc gcgtcgtttt acccgcgtgg acacggcgga
    13141 taacaaagga cgggttgcac tttggcaggg tgataagttc attcccgtga aatagatagc
    13201 tgtgcagatg gataacgaca atgcctttac gacggttctc cccaggactg aaagcccagt
    13261 ttgccttcgg catggtcttt ttgttcgttc agcccgatgc cagcgctgct gacataagtg
    13321 cgcagcaaat aggtggggtg attattccgc aggccttcag tcaggcgctt caggacggca
    13381 tgagcgtccc gctctatatt catctcgccg gtagccaggg tcgccaggac gatcagcgaa
    13441 tcggcagcgc ttttatctgg ctggacgatg gacagctacg catccggaaa atacagctgg
    13501 aagagagtga agataacgcc agtgtcagcg aacaaactcg acagcagctg atggctctgg
    13561 cgaacgcccc gttcaatgag gcccttacca tccccctgac tgacaacgcg cagctggatc
    13621 tcagcttgcg ccaactgctg ctgcagctgg tggtcaagcg cgaagcgctg ggcaccgtac
    13681 tacgctcacg tagcgaagac atcgggcagt ccagtgttaa caccctcagc agtaatctga
    13741 gctataactt gggcgtctat aacaaccagt tgcgtaacgg cgggagcaac acatccagct
    13801 atctgtcgct gaataacgtt actgcgctgc gcgaacatca tgtggtgctc gacggctcgc
    13861 tgtacgggat cggtagcggt caacaggaca gtgaattata taaagcgatg tatgaacgcg
    13921 attttgccgg tcaccgattt gccggtggaa tgctcgacac ctggaacttg cagtccttag
    13981 ggccgatgac cgccatttca gcagggaaga tttacggcct ttcctgggga aaccaggcca
    14041 gctccaccat cttcgacagc agccagtcag ccacgccagt gatcgccttt ttaccggcgg
    14101 cgggcgaagt acatctcacc cgtgatgggc ggctactaag cgttcagaac ttcactatgg
    14161 gcaatcatga agtggatacc cggggtctac cgtacgggat ttacgatgtg gaagttgagg
    14221 tgatcgttaa cggtcgcgtg atcagcaaac gcacccagcg ggtcaataag ctgtttagcc
    14281 gggggcgcgg cgtcggtgca ccactggcgt ggcaggtatg gggcggtagc tttcatatgg
    14341 atcgctggtc ggaaaacggg aaaaagacgc gaccagctaa agagagttgg ctagcaggtg
    14401 cctcgacctc cggctcattg agtacgctta gctgggcggc aacgggatat ggatacgata
    14461 atcaggcggt gggtgaaacc cgtctgacgc tgccgcttgg gggggcgatc aacgttaacc
    14521 tgcaaaatat gctggccagt gacagctcat ggagcagcat cggcagcatc agcgccactc
    14581 taccgggagg ctttagttcg ctgtgggtta atcaggaaaa aacccgcatt ggcaatcaat
    14641 tgcgacgtag cgatgccgat aaccgtgcaa tcggcggcac actcaacctg aactcactgt
    14701 ggtcgaagct gggcacattc agcatcagct acaatgatga ccgccgttac aacagccatt
    14761 attacacggc agattactat caaaatgtct acagcggtac ctttggttcg cttggcctgc
    14821 gggccggtat tcagcgctat aacaacggcg acagcaacgc caatacaggg aaatatatcg
    14881 ctctcgatct ctcgctacca ctgggcaact ggtttagcgc agggatgacc catcaaaacg
    14941 gctacaccat ggcaaacctg tcagcacgca aacagtttga tgaaggaacc attcgcactg
    15001 ttggtgccaa tctgtcacgt gccatctccg gcgataccgg tgatgacaaa accctcagcg
    15061 gtggggcgta tgcacagttc gacgctcgtt acgccagcgg aacgctgaac gtcaatagcg
    15121 cggcggacgg ctacatcaat accaacttga ccgccaatgg cagcgtcggc tggcagggta
    15181 aaaacatcgc tgccagcggg cggactgatg gcaacgctgg ggtgatattc aacaccgggc
    15241 tggaggacga cggtcagatc agcgccaaaa tcaacgggcg gattttcccg cttaacggca
    15301 agcgtaacta tctcccgctc tctccctatg gaagatatga ggtggagtta cagaacagca
    15361 aaaactcact cgacagctac gatatcgtca gcggccgcaa aagccatctg actctctatc
    15421 caggcaatgt cgctgtcatt gagccagagg tgaagcagat ggttaccgtc tccggtcgta
    15481 tccgtgcgga agacggcaca ctgctggcta acgcacggat taacaaccat atcggccgaa
    15541 cccgaaccga tgaaaacggc gagtttgtca tggacgtgga taagaaatat cccactatcg
    15601 attttcgcta cagtggcaat aaaacctgcg aagtggcact ggaactcaac caggcgcgcg
    15661 gtgccgtctg ggtcggtgat gtggtctgca gcggcctctc atcgtgggcg gcggtgacgc
    15721 agacaggaga agagaatgag agttaaccta ctaataacga tgataatttt cgcgctaatc
    15781 tggccagtaa ctgagctcag agcggcagtg agcaaaacaa cctgggcgga tgcaccggca
    15841 cgcgagtttg tgtttgtcga aaacaactca gacgacaact ttttcgtcac tcctggcggg
    15901 gcgctggatc cgcgcctgac cggtgccaac cgctggaccg gtttaaaata cactggttca
    15961 ggaaccatct atcagcaaag cctcggctac attgataacg gttacaacac cggcctttat
    16021 accaactgga agtttgatat gtggctggaa aattcaccag tttcatctcc tttaactggc
    16081 ttgcgctgca tcaactggta cgctgggtgt aatatgacca ccagtcttat cctgccgcaa
    16141 accaccgacg ccagtggatt ttatggcgcg acggtgacca gcggcggcgc gaagtggatg
    16201 cacggcatgt tgtcagacgc gttttaccag tatctgcaac aaatgcccgt cggcagcagc
    16261 tttacaatga ccatcaatgc ctgccagacc tctgtgaact atgacgccag cagcggcgca
    16321 cgctgtaagg atcaggcctc cggcaactgg tatgttcgca acgtcaccca tacgaaagca
    16381 gcaaatctgc ggttaataaa tacccactcg ctggcggagg tatttatcaa cagcgacgga
    16441 gtaccgactc tgggcgaagg gaacgccgac tgccggacac agaccatcgg cagcctttca
    16501 ggattaagtt gtaagatggt caactatacc ctgcaaacaa acggactcag caacacctca
    16561 atccatatat tcccggcgat cgccaactcg tcgttagcct cggccgtcgg ggcgtacgat
    16621 atgcagttca gtctgaatgg cagttcatgg aaaccggtga gcaataccgc ctattactac
    16681 accttcaacg agatgaagag cgcagactcg atctatgttt tcttctcgag caacttcttt
    16741 aagcagatgg tgaacctcgg gatcagcgat atcaacacca aagatctatt caactttcgc
    16801 tttcagaaca ccacatcacc ggagtctggc tggtatgaat tttctacctc caacacgctg
    16861 attatcaaac cccgtgattt cagcatcagt attatctccg atgaatatac tcagacaccg
    16921 tcgcgggagg gatatgttgg cagcggcgag tcggcactcg atttcggcta tatcgtaacc
    16981 accagcggta aaacagctgc cgacgaagtg ctgatcaagg tgaccggacc cgcgcaggtg
    17041 attggcgggc gctcctattg tgtcttcagc tccgatgacg gtaaggcgaa agtaccgttc
    17101 ccggcgacac tttcctttat tacccgcaac ggagctacaa aaacctacga tgccgggtgc
    17161 gatgatagct ggcgggatat gaccgatgcg ctgtggttga ccacaccgtg gactgatatc
    17221 tctggcgaag tggggcagat ggataagacc acagtcaaat tttcgattcc aatggataac
    17281 gccatttcgc tgcgtacggt agatgataac ggctggtttg gcgaagtcag cgcttcagga
    17341 gagattcatg ttcaggcgac gtggcgtaac attaactaag gccctgctga cagcggtctg
    17401 tatgctggcg gcacctttga cacaggcgat ttcggtcggc aatctgacat tttcgctgcc
    17461 gtccgagact gactttgtca gcaaacgtgt agtgaataac aacaaaagcg cacggatata
    17521 ccgtattgcc atcagtgcta ttgatagccc gggcagcagt gaattgcgca cccgaccggt
    17581 ggatggtgaa ctgcttttcg ccccccgcca actggcgttg caggctggtg agagcgagta
    17641 ttttaaattt tactatcatg gtccacggga taaccgcgag cgctactacc gggtctcatt
    17701 tcgcgaagtc cccactcgta accagacaag acgtagccca accggcggcg aggtcagcac
    17761 ggagccggtg gtggtgatgg ataccattct ggtagtacga ccacgtcagg ttcagtttaa
    17821 atggtccttc gacaaggtga caggaacggt aagtaacact ggcaacacat ggtttaagct
    17881 actgattaag ccaggatgtg attcgaccga agaggaaggc gatgcctggt atctacgtcc
    17941 gggagacgtg gttcatcagc ctgagttacg tcagccgggg aatcattatc tggtctataa
    18001 cgacaaattc attaagatta gcgattcctg tccggctaag cccccttcgg cggactaaga
    18061 cttatccatc ggtcgaggga agaattccac ctcagagctc caaaaatcgt ttaaatgatg
    18121 tggaagcgat cgtcaatgtc acctgtaacg atgggctttc tcatatgcac accatagtgt
    18181 tcaggagaca cggaaaggta tcaggcagcc tgctaacgag tggtttaatc acgaattagt
    18241 acgtaaaatc ggtaacggct ggaaatcatt caatactcac actatcgaaa gttccccagc
    18301 caaccgcggt acgttcttac atacgatgta ccgctgttct ctttacgatt tttagctgta
    18361 ctggtgaatt atgagcaatc tgaatccatg catgacgtgt ggtgcctgtt gtgcattttt
    18421 ccgcgtctct ttttactggg ccgaagccga cgatgctggc ggtactattc ccgccaggct
    18481 cactgaacaa atatcccctt ttcaccgatg catgagcggt accaatcaga aaaacccccg
    18541 atgtattgcc cttgcaggaa ccccgggcaa aaatgcctgc tgcacgatat ataaaaatcg
    18601 atcgtccaca tgcagagaat tcgccatgtc tggtgagaac ggagtcgtca atgaggcttg
    18661 caatcgtgca agggctaaat acgggctgac accgttataa acatacaata attaattgca
    18721 ctgccccgcc agcgataata gcggggcttc gtttttcagg ggtaacaaaa cccgatactt
    18781 cttctatttg ccagcaacaa tgcctctctt ctgtagcgtt cttcggatct acatcatcct
    18841 gaagtagcgc gatatactca ctgactatct gcatcagaat ataaaaagca atgtttttaa
    18901 cctataaaaa tggcgctgta tttgcgccat ttttatcatt caatgcatta tctgtttgag
    18961 cctaaaggga tctcagggtc tggctcatga gtaattctgt ttcgtaaatc tctgcgaata
    19021 atttcaatag accagaacca gactaaatgt ccaaaaattt cagaaacatt ctcataccac
    19081 gggagatcaa acagaggtgg cgtcagtccc atgagaggga atgaaatcat atgaacaaaa
    19141 agttgggcta aagcacctgc cagtaagccc tgccagagtt taatttttgg aaatacttca
    19201 gcgaccacac aataaccgac agcaaacact atcgaaaaga taatgtgcgt aacaccaacc
    19261 cagttaaaga catgcccggc aaaggtataa acagccgcat tgggatctgt cagccccaac
    19321 cagtctcgaa gaaaaatata cggtggattg agaaaattac gcgagcaatc aatttggcct
    19381 gcagccctga ttaatgattc cgggccacac gctgcattaa acatatccac cgggctacgt
    19441 ggcggcaatg gaacttcagc cccccacttc acgaatgcgg aaacaacgcc agcaatcagc
    19501 ccaatgaatg cagcaagacc ataacgtctg cggttcggtg gagtttgttc aaatatattc
    19561 atatctaccc tgcttgtacc attatgttat acacctcttc aggagtattc ataaaacaag
    19621 gcaaatgtaa agaactgtat tgttttgtat aacaagatgg tttcctaatc gccaatgaat
    19681 ataagctcca tcatttctcc ctatttttat attaaaagtg acagagattt gcagggtgat
    19741 gcagagctga aaatcacagg tttccttatt ggtttttgca tcgtacaact aaagcaataa
    19801 accagctccc atcatcatca acatcccggc gaacatgaat ttattagccg ggctcatagc
    19861 tgcattcgcg ttttaagaat aatcctcctg ctgtcgccga ctatgcttaa catttaaaaa
    19921 agcatcagca ctctcgcaac gcactcttat tttccccttt agaataccgg aggcctggta
    19981 tgagcaacca aggcgaatac cccgaagata atcgggttgg gaagcacgag ccgcacgatt
    20041 taagtttgac ccgtcgcgat ctgattaaag tgagcgccgc aacagcggcg accgccgtgg
    20101 tttatcctca ttctacgctg gcggcaagcg ttccggcagc tacacccgcg ccagagataa
    20161 tgcccctgac actgaaggtg aacggcaaaa ccgagcagct tgaggtggat acccgaacca
    20221 cgctactgga cgctttgcgt gaaaatctgc atttgatcgg taccaagaaa ggttgcgatc
    20281 acggacagtg cggagcctgt accgtgctgg tcaatggtcg caggcttaat gcctgcctga
    20341 cgcttgcagt catgcatcag ggggccgaga tcaccaccat tgaaggcctg ggctcgccag
    20401 ataatcttca ccccatgcag gcggccttta tcaagcatga tggcttccag tgcggctact
    20461 gcacctccgg gcaaatttgc tcatcagtag cggtgctaaa agagattcag gacggcattc
    20521 ccagtcacgt cacggtcgat ttggtttccg ctccagaaac aactgccgat gagatccgtg
    20581 aacgtatgag cggcaacatc tgtcgctgtg gtgcatacgc taacatcctt gccgccattg
    20641 aagatgctgc gggggagata aaatcatgaa ggcgtttacc tatgaacgag tgaacacccc
    20701 agccgaggcg gcacttagcg ctcagcgcgt acccggcgca aaatttatcg cgggcgggac
    20761 caatctgctg gacctgatga agctggaaat tgaaacgccc acccacctta tcgatgtgaa
    20821 cggcctcggg ctcgataaga ttgaagtgac cgacgcgggt gggctgcgca tcggcgcact
    20881 ggtacggaac accgacctgg cggctcacga gcgcgtgcgt cgtgattacg cggtactctc
    20941 ccgcgccctg ctcgctggcg catctggtca gttacgtaat caggcaacca ccgcaggtaa
    21001 tctgctccag cgcacgcgct gcccctattt ttacgacacc aatcagccct gcaataagcg
    21061 cctgcccggg agcggctgcg cggcgcttga aggctttagc cgtcagcacg cggtggtagg
    21121 cgtaagcgaa gcctgcattg ccacccatcc gagcgatatg gcggtcgcaa tgcggttgct
    21181 ggatgcggtg gtggaaacca tcacgccgga gggaaagact cgcagtatca cactggctga
    21241 tttttatcac cctccgggaa aaacgccgca cattgaaacc gccctgcttc ccggtgagct
    21301 tatcgttgcg gtgacgttac ctccgccgct cggcggaaaa catatctacc gtaaggtgcg
    21361 cgatcgcgcc tcctacgcct ttgcccaggt atcggtcgcg gcgattattc accctgacgg
    21421 cagcgggcgc gtcgcgctgg gcggagtagc acataagccc tggcgcattg aggctgcgga
    21481 tgctcagcta tcccaggggg cgcaggccgt atatgacacg ctgttcgcca gcgcccatcc
    21541 caccgctgaa aacaccttta aactcctgtt ggcgaagcga acgcttgcct ccgtactggc
    21601 tgaagcgagg gcacaggcat gaaatttgat aaacccgcag gggaaaaccc gcaggggaaa
    21661 acccgatcga tcagctgaag gttgtcggtc gtccccatga ccgcatcgac ggaccgctga
    21721 aaactaccgg cacggcacgc tacgcctacg aatggcatga agaggccccc aacgccgcct
    21781 atggctatat cgtcggttcc gccattgcca aaggacgcct caccgccctt gatacggacg
    21841 ccgcgcaaaa agcgccgggc gtactggctg tcattaccgc cagtaacgcc ggggcactcg
    21901 gcaaaggcga caaaaacacc gccagactgt taggcggccc cactattgag cactatcatc
    21961 aggccattgc gctggtagtg gccgagacct tcgaacaggc gcgagcggcg gcctcgctgg
    22021 tgcaggcgca ctatcgccgt aataaaggag cttactccct ggcggacgaa aaacaggccg
    22081 tcaatcagcc gccggaaggc acgcccgaca aaaacgtcgg tgactttgac ggggctttct
    22141 cctccgctgc ggtgaagatt gatgctacct acacgacccc ggaccagagc catatggcga
    22201 tggagccgca tgcctcgatg gccgtctggg atggaaataa gcttactctc tggacctcaa
    22261 atcagatgat tgactggtgc cgcaccgatc tggcaaaaac gctgaaagtg cccgtggaga
    22321 atgtgcgtat tatctccccg tatatcggcg gagggtttgg cggcaagctg ttcctgagaa
    22381 gcgatgcgct gctggcagcc ctcgccgccc gagcggtgaa acgtccggtt aaagtgatgc
    22441 tcccccgccc cactattccc aataacacca cgcaccgccc cgcaaccctt cagcacctgc
    22501 gtattggtgc tgaccagagc gggaaaatca ccgctatctc acatgaaagc tggtccggaa
    22561 acctgcccgg cggcacgccg gaaacggcgg tacagcaaag cgaattactc tacgccgggg
    22621 caaaccgtca taccggcctg cggctcgcca cgcttgattt gccggaaggg aacgccatgc
    22681 gtgcgcccgg cgaagccccc ggtctgatgg cgctcgaaat cgcgatcgac gaactggcgg
    22741 aaaaagcggg catcgatccc gtcgagtttc gcatcctgaa tgacactcag gttgaccccg
    22801 ccgacccgac gcgccgcttc tctcgccgtc agcttatcga gtgcttgcgc accggagcgg
    22861 ataaatttgg ctggaagcag cgcaacgcca cacccggaca ggtgcgcgac ggggagtggc
    22921 tagtcggcca cggcgtcgcg gcgggctttc gcaataatct gctggaaaaa tcgggggctc
    22981 gggttcacct cgaaccaaac ggcaccgtta ccgtggaaac ggacatgacc gacattggca
    23041 ccggcagcta caccattctg gcccagacgg cagcggaaat gcttggcgta ccgctggagc
    23101 aggttgcggt tcacctcggc gattccagtt tcccggtttc tgcgggttct ggtggacaat
    23161 ggggcgcgaa tacctccacc tccggcgttt acgccgcctg tgtgaagctt cgcgaaatga
    23221 ttgcctcggc agtcgggttt gatcctgagc agtcgcagtt tgccgacggc aagattacca
    23281 acggtacccg aagcgccatg ctacatgagg ccaccgcagg cggcagactg atagcggaag
    23341 agagcattga attcggaaca ctgagcaagg agtaccagca gtcgaccttt gccgggcatt
    23401 ttgtggaggt cggcgtgcat agcgcgacgg gagaagttcg ggtccggcgt atgctcgctg
    23461 tgtgtgctgc aggacgcatc ctgaatccga aaactgcacg cagccaggtc attggcgcaa
    23521 tgactatggg catgggcgcg gcactgatgg aggagctggc ggtggatgac cgtttgggct
    23581 acttcgttaa tcacgatatg gcggggtatg aggtgccggt ccatgcggat atcccaaaac
    23641 aggaggtgat tttcctggat gataccgacc ccatatcctc cccgatgaag gccaagggtg
    23701 tcggtgagct gggcctgtgc ggcgtgagcg cggctatcgc caacgcggtg tataacgcca
    23761 ccggtattcg ggtacgcgat tatcccatca ctctggataa gctgctcgat aagctgccgg
    23821 atgtggttta aggagaaaca atgtcatacc cgctttttga taaagacgaa cactggcata
    23881 agccagagca ggcgtttctc accgatgacc accggaccat tctgcgcttc gccgtagagg
    23941 cgctaatgtc cggtaaagga gcggtgctgg tgacgctggt ggagatacgc ggcggcgcgg
    24001 cgcgcccgct cggggcgcag atggtggtgc gcgaagatgg tcgttactgc ggttttgtct
    24061 ctggcggctg cgtggaggcc gctgccgctt ttgaagcgct ggagatgatg ggctcaggcc
    24121 gcgatcgcga aattcgctat ggcgaaggtt cgccgtggtt tgacatcgtt ctgccctgcg
    24181 gcggtgggat cacgctgacg ctccataaac tacgctcggc acagcctctg ctcgccgtgc
    24241 tgaaccgcct ggaacagaga aaaccggcgg ggctgcgcta cgatccgcaa gcacaatcgt
    24301 tggtgtgcct gcccacgcaa acccgaacgg gctggaatct caatggcttt gaggtggggt
    24361 tcaggccatg cgtcaggctg atgatttacg gacgttctct tgaggcgcag gcaaccgcga
    24421 gtcttgcagc agccacaggc tatgacagcc atatcttcga tctttttccg acctcagcca
    24481 gcgctcagat cgataccgat acggcggtca ttttgctgtg ccatgatctc aaccgggagc
    24541 tgccagtgtt gcaggccgcg cgagaagcaa aaccctttta tctcggcgca ttgggcagct
    24601 atcgaaccca cactttacgt ctgcaaaagc tccacgagct gggatggtcc agggaggaga
    24661 cagcgcaaat ccgggcaccc gtcgggatat ttcccaaagc ccgggatgcg catactctgg
    24721 cactctccgt gctggcagaa gtcgcctctg tacgtctcca tcaggaggag gattcatgcc
    24781 tgcccccgtc gtcctgatcc ttgcggccgg gcgtggagag cgctttctcg cctccggggg
    24841 aaatacccat aagtgtatcg gctggcgtca gtccccggag gttgcgcctt atcgctggcc
    24901 atttgaagaa aacgggagaa ctttcgacct tgcgattgaa ccgcagatta cgactaatga
    24961 tctgcgtctg atgttgcagc tggctcttgt cggtgaagga ataacaattg ccactcagga
    25021 aactttcagg ccatatattg aaagcggtaa gcttgtatcg ctgcttgatg actttcttcc
    25081 acaatttcca ggcttctatc tgtatttccc acagcgtcgc aatattgcac caaagctccg
    25141 cgccctgatt gaccacgtca aggaatggcg gcagcaattg gcttaaatgt ctgcacctgc
    25201 attgcctgat gtcagaacag tattttgatg aattgccagg gttacaatgg cacaaatacg
    25261 gcacaggagg aaacgtggtg tatttaaata tggggtaact tattgattta aatggtgccg
    25321 ataataggag tcgaacctac gaccttcgca ttacgaatgc gctgctctac caactgagct
    25381 atatcggccc tgaaaggaca tgttcacgaa cgtgaatcac ggtggacaag gttaaaacta
    25441 accgggcgat gcgtcaatgg ccttgtgaat caaatggcta cttttgcatc acccggtttt
    25501 atttacgcac gaatggtgta atcaccaata ccgatccact tgtaagtggt cagtgcttcc
    25561 agccccattg ggccacgcgc gtggagtttt tgtgtgctta ccgccacttc cgcacctagt
    25621 ccaaactggc cgccgtcggt aaaacgcgta gaggcgttaa cgtaaacagc ggacgaatcc
    25681 acttcgttaa caaaacgctg ggcgttgcgc atatcgcggg tcaggatcgc atcggagtgt
    25741 tgtgtgccgt gttcacgaat atgggcgatg gcatcgtcaa gatcactgac gattttgacg
    25801 ttcaaatcta atgacagaaa ctcatcgtca tactcttccg ctttaacagc caccaccttc
    25861 gcggggcctg tctgcaactg cgccagcgca gctgcatctg cgtgtaatgc cacgccgctt
    25921 tcctccattt gtttgcttaa tgcgggcagg aagctatcgg cgatgttttt attcaccagc
    25981 aacgtttcta ccgtattaca tgtgctcgga cgctgagttt tcgcgttgac gatcactttt
    26041 aatgcttcag caatctctac actttcatca acataaatat ggcatacgcc tataccacct
    26101 gtgatcaccg ggatcgtcga ctgttcgcgg cacagtttat gcaaaccagc gccaccacgc
    26161 gggatcagca tgtcgatgta tttatccata cgcagcattt cactgaccag cgcacggtca
    26221 ggattatcaa tcgcctgcac ggcacccacc ggtaagccac aggatttcag ggcgtcctga
    26281 atcaccgcca ccgttgccgc gttagtgcga caggtttctt taccgccacg cagaatcact
    26341 gcgttaccgg ttttcaggca cagcgaagcg acatcaaccg tcacgttcgg gcgcgcttca
    26401 taaatcacgc caataacccc cagcggtacg cgacgacgct caagacgcag gccgctgtcc
    26461 agtacgctgc catcgattac ctgccccacc ggatcggcga ggttacacac ctggcgcaca
    26521 tcatcggcaa tgcctttcag ccgtgcgggc gtcagtgcca gacggtcaag catcgcttcg
    26581 ccaaggccat tggcacgcgc gtcagcaaca tcctgggcgt tagcgttgag gatgatttcg
    26641 ctttgtgctt ccagttcatc ggcgattttt tccagcacgc gatttttttc gcggctggag
    26701 agttgcgcta atttatacga ggcttgcttc gcggcaatgc ccatttgttc cagcatcagc
    26761 ctgctcctta acgggtaatc atgtcatcac ggtgaacggc aaccgggccg tattcatatc
    26821 ccagtattgc atcaatttct tgcgagtggt gcccggcaat acggcgtaat gcatcgctgt
    26881 tgtaacgact gacgccgtgg gcgatatcgc gaccttcgag gttgcaaatg cggatgactt
    26941 caccacgcga gaaattgcca gtcacgcttt taatgccttt cggcaacagg gagctgccgc
    27001 gttcaagaat ggcggcagtt gccccttcat ctaccgtgat ttcacccgcc ggcggcgcac
    27061 cgaaaatcca gcgtttacgg ttttcaagcg gagtcgcctg ggcatggaac agcgtaccga
    27121 cggaaatgcc ttccatcaca tcaccaataa cgcccggctt gctgcccgcg gcaataatgg
    27181 tgtcgatacc cgcacggcaa gccacgtcag cggcctgcaa tttggtactc atgccgccag
    27241 ttccgaggcc tgaaacgctg tcaccggcaa tcgcgcgcag tgcgtcatca atgccgtaaa
    27301 catctttaat cagttctgcc tgcggattgc tgcgcggatc agcggtatac aaaccttttt
    27361 gatcggtcag cagcaacagt ttatcggcac ccgccagaat cgccgccagc gcagaaaggt
    27421 tatcgttatc gccgacctta atctctgccg tagcgacagc atcgttctca ttgattaccg
    27481 gaacgatatt gttatcgagc aacgcacgca gggtgtcgcg ggcgttcagg aagcgttcac
    27541 ggtcttccat atcagcacgg gtcagcagca tctgcccgac gtgaatgcca taaatcgaaa
    27601 acagctgttc ccacagttga atcagtcgac tctgccctac cgccgccagc agttgtttcg
    27661 aggcgatagt cgctggcagt tccgggtacc ccaggtgctc acgtccggcg gcgatcgcgc
    27721 ccgacgtcac aataacaatc cgatgcccgg cggcatgtaa ctgcgcgcac tggcgaacaa
    27781 gttcaacgat atgggcacgg ttcagacggc gcgatccgcc tgttagcaca ctggtgccga
    27841 gttttaccac cagcgtctgg ctgtcactca tgattctctg ccattcaatt ttaggaaaaa
    27901 tgatatcaaa cgaacgtttt agcaggactg tcgtcggttg ccaaccatct gcaagcaaag
    27961 catggcgttt tgttgcgcgg gatcagcaag cctagcggca gttgtttacg cttttattac
    28021 agatttaata aattaccaca ttttaagaat attattaatc tgtaatatat ctttaacaat
    28081 ctcaggttaa aaactttcct gttttcaacg gggctctccc gctgaatatt cgcgcgttaa
    28141 ttaaaatcag gaatgaaaat gaaaaagagc actctggcat tagtggtgat gggcattgtg
    28201 gcatctgcat ccgtacaggc cgcagaaata tataacaaag acggtaataa actggatgtc
    28261 tatggcaaag ttaaagccat gcattatatg agtgataacg acagtaaaga tggcgaccag
    28321 agttatatcc gttttggttt taaaggcgaa acacaaatta acgatcaact gactggctat
    28381 ggccgttggg aagcggagtt tgccggaaat aaagcggaga gtgatactgc acagcaaaaa
    28441 acgcgtctcg cttttgccgg attgaagtat aaagatttgg gttctttcga ctatggccgt
    28501 aacctgggcg cgttgtatga cgtggaagcc tggaccgata tgttcccgga atttggtggc
    28561 gactcctcgg cgcagaccga caactttatg accaaacgcg ccagcggtct ggcgacgtat
    28621 cggaacaccg acttcttcgg cgttatcgat ggcctgaact taaccctgca atatcaaggg
    28681 aaaaacgaaa accgcgacgt taaaaagcaa aacggcgatg gcttcggcac gtcattgaca
    28741 tatgactttg gcggcagcga tttcgccatt agtggtgcct ataccaactc agatcgcacc
    28801 aacgagcaga acctgcaaag ccgtggcact ggcaagcgtg cagaagcatg ggcaacaggt
    28861 ctgaaatacg atgccaataa tatttatctg gcaacttttt attctgaaac acgcaaaatg
    28921 acgccaataa ctggcggctt tgccaataag acacagaact ttgaagcggt cgctcaatac
    28981 cagtttgact ttggtctgcg tccatcgctg ggttatgtct tatcgaaagg gaaagatatt
    29041 gaaggtatcg gtgatgaaga tctggtcaat tatatcgatg tcggggctac atattatttc
    29101 aacaaaaata tgtcagcgtt tgttgattat aaaatcaacc aactggatag cgataacaaa
    29161 ttgaatatta ataatgatga tattgtcgcg gttggcatga cctatcagtt ttaatgatta
    29221 ttgccggatg tgatgcatcc ggcacatttc actcacgccg ttaacttcac cggctcgtca
    29281 cgaaaatcat ccgccggttc cagcttcaga ttcagcgtcg tcagcagttc acgcagcttc
    29341 tcgtgaaact cacgcagggt gtgctccagt cgttcaacca cttcagtgtc ttttaccgga
    29401 acgctcttcc agtcgcctgc tttatcgaac agaccaaact ggtagctgta ggtaaaacgg
    29461 gattcctgcg cttccagctc catccaccag ccccagaatt cacgcacttc cggtgccggt
    29521 ttcacgttga cgcatacagc cagacaatcg aaaaagaatc gattatcttc gcacttacct
    29581 tcacgaatat acgggcctag tgcggtaaat tttttgatca atctgctctt cgggtgtcca
    29641 ctcggtaacg tcattgctat ctcctgttgt gatgcaactg ttttaccaaa ttggcaaaat
    29701 ttagcaaatt tttaacacaa gcgtttttcg atccagccgg tgatttcctg aagacctttg
    29761 tcaaaattcc gatacaccgg gttaaatggg atctctaata atttaccgtc agcagatgat
    29821 gaggtgatta agcgtgagtc ctcttccggg ctgaacggat cgttcttcca gtagcctgat
    29881 aacattggcg ttgggcagcg acgtcccagc aatccttgca cttttaatga atagcgattc
    29941 agctccacgc gcaacgcgtc atcggaagca tcatgcatcc ccaaacgact cgccagaacg
    30001 tcaagataca tttccggcac ctgttgctgg cacttaaaat cactcaacag ggtatgaact
    30061 accggaccaa gacaggcaac cgctttcaga cgcggcgatt caaggtatgc cagacgcacg
    30121 gcaacgttag cgccgaaacg gaaaccaaag gccgcgacgc gagtgtgatc cacccacggt
    30181 acgttaggca gcgcctttaa gacgtgctga tgcaacaggc tggagtcctg ggtgagcttc
    30241 cattttgaag aaaagcccac cgacggcata tcaatagtca gcatcgcaat gccgcgcggc
    30301 gcaaaataac gttcatacag gctgtaatag tccgtctgca tcgcatccag accaccacac
    30361 attaatactg tcgggaacgg gccatcgcct ttcggcatat gcaaaaagcc ggtgatgggc
    30421 gcaccgccgg gtacggtaaa ctccatctgc cgcatcgtgc ccggcagacg ctgggcggcc
    30481 tcttcatagg cgcggtttga taaagcctgc gcttgctcgg ccaggtcatc tcctttcaga
    30541 tgaggatagg cggcaatgtt gtacaacgta gccgcatgca gccagtgtcg accactgagt
    30601 tgcggatctt cttccgcaca ggctttttgt tgccacacca tcgcctgggt ggcccactca
    30661 taaatccagt tgccaccacg gtagccaatc acggtatcgt ataaatcatc atcggtacgt
    30721 tcagcatcgc tcatcacaat acgtgcctgg acgtcgagga tttcacgtgg gtcaatgccg
    30781 cgccagatcc acatcagacg gttaatcatg cgataccagt gagggatggt tttaccatca
    30841 agggccgact gcacaggcgg ttgtgcgccg tgattaaagc ggcggactag cgtcgaggtt
    30901 tcaggatgtt taaagcgggg tttgaacagg gtttcgctca ggtttgcctg tgtcatggat
    30961 gcagcctcca gaatacttac tggaaactat tgtaacccgc ctgaagttaa aaagaacaac
    31021 gcccggcagt gccaggcgtt gaaaagatta gcgaccggag attggcggga cgaatacgac
    31081 gcccatatcc cacggctgtt cgatccaggt atcttgcggg atatcaacaa cataattatc
    31141 aaccagcgga cgaccagccg gttttgcaaa gatggtgaca aagtgcgctt ttggatacat
    31201 ttcacgaatc gcaaccgcag taccaccggt atccaccagg tcatcaataa cgatgaagcc
    31261 ttcgccatcg ccttctgcgc gtttcagcac tttaagctcg cgctggttgt cgtgatcgta
    31321 gctggaaata caaacggtat cgacatgacg aatacccagt tcacgcgcca gtaacgcacc
    31381 cggtaccaga ccgccacggc ttacggcaat aatgcctttc cattgttcag aaggcatcag
    31441 tcggcttgcg agtttacgtg catggatctg caacatgtcc caggtgacga tgtatttttc
    31501 gctcatgtga agtgtcccag cctgtttatc tacggcttaa aaagtgttcg aggggaaaat
    31561 aggttgcgcg agattataga gatctggcgc actaaaaacc agtatttcac atgagtccgc
    31621 gtctttttac gcactgcctc tccctgacgc gggataaagt ggtattctca aacatatctc
    31681 gcaagcctgt cttgtgttga caacattttc tgctaaccct gtgacctgca atactgtttt
    31741 gcgggtgatc gacaaggaga cttaacgtgt ctgaactgtc tcaattatct ccacagccgc
    31801 tgtgggatat ttttgccaaa atctgttcta ttcctcaccc gtcctatcat gaagagcaac
    31861 tcgctgaata cattgttggt tgggcaaaag agaaaggttt ccatgtcgaa cgcgatcagg
    31921 taggtaatat cctgattcgt aaacctgcta ccgcaggtat ggaaaatcgt aaaccggtcg
    31981 tcttgcaggc ccacctcgat atggtgccgc agaaaaataa cgacaccgtg catgacttca
    32041 cgaaagatcc tatccagcct tatattgatg gcgaatgggt taaagcgcgc ggcaccacgc
    32101 tgggtgcaga taacggcatt ggtatggcgt ctgcgctggc ggttctggct gacgaaaacg
    32161 tggttcacgg cccgctggaa gtgctgctga ccatgaccga agaagccggt atggacggtg
    32221 cgtttggctt acagagcaac tggttgcagg ctgatattct gattaacacc gactccgaag
    32281 aagaaggtga aatctacatg ggttgcgcgg ggggtatcga cttcacctcc aacctgcatt
    32341 tggatcgtga agcggttcca gctggctttg aaaccttcaa gttaacctta aaaggtctaa
    32401 aaggcggtca ctccggcggt gaaatccacg ttggcctggg taatgccaac aaactgctgg
    32461 tgcgcttcct ggcgggtcat gcggaagagc tggacctgcg ccttatcgat ttcaacggtg
    32521 gcacactgcg taacgccatc ccgcgtgaag cctttgcgac cattgctgtc gcagctgata
    32581 aagtcgacgc cctgaaatct ctggtgaata cctatcagga catcctgaaa aacgagctgg
    32641 cagagaaaga gaagaatctg gccttgttgc tggactctgt agcgaacgat aaagctgctc
    32701 tgattgcgaa atctcgcgat acctttattc gtctgctgaa cgccaccccg aacggtgtga
    32761 tccgcaattc cgacgtggca aaaggtgtgg tcgaaacctc cctgaacgtc ggtgtggtga
    32821 ccatgactga caataacgta gaaattcact gcctgatccg ttcactgatc gacagcggta
    32881 aagactacgt ggtgagcatg ctggattcgc tgggtaaact ggctggcgcg aaaaccgaag
    32941 cgaaaggcgc atatcctggc tggcagccgg acgctaattc tccggtgatg catctggtac
    33001 gtgaaaccta tcagcgcctg ttcaacaaga cgccgaacat ccagattatc cacgcgggcc
    33061 tggaatgtgg tctgtttaaa aaaccgtatc cggaaatgga catggtttct atcgggccaa
    33121 ctatcaccgg tccacactct ccggatgagc aagttcacat caaaagcgta ggtcattact
    33181 ggacactgct gactgaactg ctgaaagaaa ttccggcgaa gtaattattt gatttgctgc
    33241 cggatggcgt ttaatcgcct tccggcagtt tcatccttca ttatccttcg ataaaagcca
    33301 tccctgtaaa tgtccgtcgc gggttgccac gttcaatctg gtgatggaac attcgccgct
    33361 gcgatttcag cgccgcgcta ttttcctgtt gctgctgctc cagcttccag gcaatcagca
    33421 atcgcgccag ccgtttgtta gcatgcccag aaaccagttt ttgcgcccat gatgagaccg
    33481 atacggactc ggacaaatcc actgaatagt gccgcaccag ctttcgctta atgcccatgc
    33541 gttatcgcca tccagagaaa ccagcgccga acgcagcgtg tcagagtagc ggcccgtttc
    33601 tgtttccagc accgttaccg cgacgtcttg ccggataaaa acgaccaaca gagaagaacg
    33661 ctgcgccaat cggatagccg cgtccgggat gtaaatctgg catccccact acgcgctgca
    33721 tgttgggtaa atttgccgtg gtgtgtaatt gttggatcgc taaactctcg atccacaggt
    33781 catcagatgc gatggtaaat accgcatcgg ataagggacg aatatatttg cccatgtacc
    33841 attccattta tactcgtcat acttcaagtt gcatgtactg cgactgcgtt cgttcacccc
    33901 agtcacttac tttagtaagc tcctggcgat tcactcgctt gtcaccttcc tgcaactcga
    33961 attatttaga gtatataaat attaaaaatc aggaaatggc agacgaatcc agggcaagaa
    34021 aaaaagaatt aatcctggaa aggtctgcaa agagtcattg ataaaggatt tgtcatgatt
    34081 tacctccttg cagtttgtgg aataagaaaa cgcgcacata ccaggcgggc gttattttca
    34141 ttgcaagctg gatttaatgt tgcggtttat atcgcatata aaaattagta aaccacgctc
    34201 cccggcattc aacgtgctgc tgcttaacta tctgaaaacc ataacgttca aaaaagggtt
    34261 ttgcggttat gctggcgtcc accgtaagtt cggattcaga cttaatcaaa ggttttaaca
    34321 aggcgctggc aaccccgcgg cgggtgtatt cagggtcaac aaataacata tcgatatgac
    34381 gttcaatgcg ggaaataaaa ccaaccggtt gtgcattaat gactgcaacc cgcacttgtg
    34441 atttcgcgag tttctccttc cagcgagatt cgtcaatctg cgcccaggcg gcaatttgtt
    34501 gtggtgaata atgctgactg gcggtcatca taaccgctct aatgaaaata gcgcatagtt
    34561 gctgaaaatc gccaggctga tagtttctta tttgtatgtt attcataatc ccagcaccag
    34621 ttgtctttcc atttgagggt caagcaacgt cacatgcagc cccaccagtc gcacaccgcg
    34681 cccgccgcgg cgttcatccc aggttttacg cgcggtggcg attagatcag ctttattcag
    34741 ccgcggccag acgtgctcct gggtggtttg ctgaaaatcg tcgaacttta atttcacccc
    34801 ctggcgagca atcagtaaat caggttttac ctttgccaga cggcgttcaa gttccggata
    34861 cagccgctcg ataatcgctt cacattcaga ccagtgatga atatcttccg ccatcgtgcg
    34921 ttccacaccg acggattttc gcaaccgttc gctgttaacg tcgcgttcgt caatcccctg
    34981 actacgctcc cacaaaatgc ggccaaattt gccaaagcgt ttaagcagaa tcaccagatc
    35041 acacttttgc acatcaccgc aggtccgcag ccccatcgct tccagttttg ccgccgagac
    35101 tttgccgacg ccggggattt ttgccaatgg taaggtttgc agaaatgccg gaacttctgc
    35161 cggagtaatc acaaactggc cgttgggttt attcatgtcg gaggcgattt tggcgagaaa
    35221 cttgaccggc gcaacgcccg cagacgccgt caggtgcagc tcgttgaaaa tcgtctggcg
    35281 gatttcctgg gcgatgaggg tcgcagaacc gtggcaatgg acgctatcgg tgacatcgag
    35341 ataagcctca tccagtgaca acggttcaat gcgcgaggtg tagcgcgaga agatttcacg
    35401 gatatgattt gaggcttctt tgtaggcgtc aaagcgcccc ggaagcaagg tgagatgtgg
    35461 gcataatttg agcgccatcc cagtaggcat agcgctacgt acgccaaatt tacgcgcggg
    35521 ataattggcg gtgctgatca ccccccgacg ttcgcggctg ccgccaatag caatagggat
    35581 atcgcgcagg gcgggattgt cgcgcatctc cactgcggcg aaaaagcagt ccatatccac
    35641 atgaatgatt ttacgcattg ctcacctctc aacactggta aagtatacag tgatttcagg
    35701 gtttgagaaa tgcgtaaaga ttcagcattt agcgcattcg ctgcgaaagc acctgctgct
    35761 tatccagctt ttgcaccagc ggctgcacca ctttatcccc atgctgacca aagtattgat
    35821 acagcgtatc ggacgcactt ttggtcagca ccataatctc aatacgccga ttgcccgcgc
    35881 tttgcggatt tttggcatcc agcagcatct ggtccgccat tgcgcttacc tgcatcactt
    35941 tatcttccgg cattccggcc tcttccagca cccgacgagc tgaaagcgcg cgatcgcccg
    36001 aaaggttcca gttgttgtag atattgtttt tgtaggccat cgcatcggta tgcccgataa
    36061 taataatttt gttatcgagc gagtcgaaca ctggcgcgag ctccaccagc aaagttttaa
    36121 agaacggcat aatctgcgcg ctgccgcgtt cgaacatatt gcggttctgg tcatctttaa
    36181 tcagcacgcg caatccctgg ggaacaatct ccatctccag attcgcttcc atatgcgcat
    36241 cacgggcaat ggtgttgata ctggttgcca gctcgccaag ctcggtggcg gatttcttct
    36301 tcagcagcgc tgttttctcg ttgacgtctc gcgctttttt ctccgtctct tccggcaccg
    36361 ccacggtggc aggttttggc ggatgagacg ggctgatttt atttaacggc gacaatccgc
    36421 cgccactgaa aatcgactgc ccgtgcagcg cggcgataat gctttcacgc tcggatttac
    36481 tgacgctgtt gacaatcaac tttatcgtcg tcaccaaagg ggccgagcgt atttccgagg
    36541 tttctgcccg ctttaccctg gacgcgatgc ccggcaaaca gatggcgatt gacgccgacc
    36601 ttaacgccgg gttgatcaac caggcgcagg cgcaaacccg gcgtaaagat gtcgccagcg
    36661 aggccgattt ctacggcgcg atggacgggg cgtcaaagtt tgtgcgcggg gatgccatcg
    36721 ccgggatgat gattctggca atcaacctga tcggcggcgt ctgtatcggg atcttcaaat
    36781 acaacctgag cgccgatgct gccttccagc agtatgtgct gatgaccatc ggcgacgggc
    36841 tggtggcgca gatcccttcc ctgctgctct ccaccgcggc ggcgattatt gtcacccgca
    36901 tcagcgacaa cggcgatatc acccatgacg tgcgccacca gctgctggca agcccgtcgg
    36961 tgctctacac cgctaccggg atcatgttcg tgctggcagt ggtgccgggg atgccgcacc
    37021 tgccgttttt gctgttcagc gccctgcttg gctttaccgg ctggcggatg agcaaacgcc
    37081 cgcaggcggc ggaggcggaa gaaaaaagcc tcgaaacgct gacccgcacc atcaccgaaa
    37141 ccagcgagca acaggtcagt tgggagacga taccgctgat cgagcctatc agcttaagcc
    37201 tcggctacaa gctggtggcg ctggtggata aagcgcaggg caacccgctc acccagcgga
    37261 ttcgcggcgt gcggcaggtg atatccgacg gtaacggcgt gctgctgccg gagatccgta
    37321 ttcgggaaaa cttccgcctc aagcccagcc agtacgctat tttcatcaac ggcattaagg
    37381 ctgatgaagc ggatattccg gcggataaac tgatggccct accctccagc gaaacctacg
    37441 gcgaaattga cggcgtgctg gggaacgacc cggcgtacgg gatgccggtg acctggatcc
    37501 agcccgcgca gaaagcgaag gcgctgaata tggggtatca ggtgatcgac agcgccagcg
    37561 tgattgccac gcatgtgaat aagattgtgc gtagctatat tcctgattta tttaactatg
    37621 acgatattac gcagttgcat aaccgtttgt cgtcgatggc accgcgcctg gcggaagatt
    37681 taagcgcggc gctgaattac agccagttgc tgaaagtgta ccgcgcgctg ctgaccgaag
    37741 gcgtttccct gcgcgatatc gtcaccattg ccaccgtgct ggtcgccagt agcgcggtga
    37801 ccaaagatca tattctgctg gcagctgatg tgcgcctggc gctgcggcga agcattaccc
    37861 atccgttcgt tcgcaagcag gagctgacgg tgtatacgct gaataatgag ctggaaaatc
    37921 tgctgaccaa cgtggtgaat caggcgcaac agggcgggaa agtgatgctc gacagcgtgc
    37981 cggtcgatcc gaatatgctc aaccagttcc agagtacgat gccgcaggta aaagagcaga
    38041 tgaaagcggc ggggaaagat ccggtgctgc tggtgccgcc tcagctgcgc cctttgctgg
    38101 cgcgttatgc aaggttgttt gcgccggggc tgcatgtgct gtcgtataac gaagtgccgg
    38161 acgagctgga gttgaagata atgggggcgt tgatgtaagt atccggcatt ggtgcacaac
    38221 tgccggatgc ggcgtgaacg ccttatccgt cctactgttc aggcacgatt cgtaggccgg
    38281 ataagcgcaa gcgcatccgg caccagcgca caaccgccgg atgcggcgta agcgccttat
    38341 ccggcctacc gatccagcac aggtttgtag gcatgataag acgcgtcagc gtcggatcag
    38401 gcatctgcgc ataaccgccg gatgcggcgt aaacgcctta tccggcctac cgatccggca
    38461 caggtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg cataattata
    38521 aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg atgggatata
    38581 accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact tgctttaccc
    38641 aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa tctttggtat
    38701 tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca caagcatggg
    38761 taaattgctt tttaatttcc cgccagcgcg aggaaaaatc atcatcgcct tcaggtaatg
    38821 tccagataca gtgcatatgc tctggcaaaa cgacccaggc gttgatttca aaaggcctgt
    38881 ctcgcttaac tttaataatg gcgtgacgga gcatctggta ctgggtggtc aagagttggc
    38941 ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg taataacgac
    39001 gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa aggtcggtaa
    39061 tgaggtgatg gaataacagg aaagcggctc gcaaaaacgc acagatttgt aggcatgata
    39121 agacgcgtca gcgtcgcatc aggcatccgt gcacaaccgc cggatgcggc gtaaacgcct
    39181 tatccggcct acaaaatcgt ctcttccgtc aagatccgcc gcgcgcccaa aaaatgatcc
    39241 tgccagaaag gttcggctaa ccggcttacc cgaatgtttt ccccggtgcg cggcgactcg
    39301 ataaactgcc catcgcccag atacaccccc atatgatcgg caatttcacg gctgtggata
    39361 tggaaaaaca gcaaatctcc ccggcgcagg tcgttattcg ccacaatcgt tgcccgatga
    39421 tagtgataca tctcattggc cgtgcgcggg agcttcgcct caaggatctt gttgtaggca
    39481 taaaaaacca acccgctaca gtcgaagcct tgatcgggcc gcgtgccgcc ccagacgtaa
    39541 ggcttgccaa gctgttgctc cagacggtga atcgccactt cggtgatgtt atgcagatgg
    39601 tcgctgctta aaaagtgatt gttctccgcc agcgcctgcc agcgtcggtc gctcgccttg
    39661 agcggcgcag gaaaccattc tggatgctgt tttatctgct cccggttgtg ctggcgtagc
    39721 gcccttctgc tttcggcatt gccttccaca atatagctgg cctgtttttt cagatgggtt
    39781 tggtactgtt ttaataaacg cgcacggttt tgcatccgct ggcgggcggc gtagctgaaa
    39841 gagatatgtg atgtggtggc gctggcgaat aaagggagca aaacgcataa cgaaaacacg
    39901 cctggatgca gaaatctgcc gagcaagaac gaagacatga aagacataaa ccggctgaat
    39961 gaaggtgaga attttaaaag accggaggag taaacgggat tattaattgc ttatttgcat
    40021 attactattg aggtttcata atcttaggaa ataaatgata catgcgcttt tcggactatt
    40081 gaaaactgcc cctgtgctaa atttgtaaac cctgcgccga caggcttgcg taaaactctt
    40141 ttggtgataa tgtgagcctt ccaaatccag actattccga tttgggatcc attttgccgt
    40201 cctggcgtgt gtaaccgttt tatcaaggaa taagcagtat gcgtaaaatc gcattaattc
    40261 ttgcgatgtt gttgatcccg tgcgtttcgt ttgccggttt gctgggcagc agtagctcga
    40321 ccacgccagt gagcaaagag tataagcagc agttgatggg atcccctgtc tacatccaga
    40381 tcttcaagga agaacgtacg ctcgatctct acgtcaaaat gggcgagcaa tatcagctgc
    40441 tcgacagtta taaaatctgt aaatattccg gcggcttagg gccaaaacag cgtcagggcg
    40501 atttcaaaag cccggaaggg ttttatagcg tccagcgtaa tcagttaaaa ccagacagcc
    40561 gttactacaa agcgattaat attggtttcc ccaatgccta tgaccgtgcg catggttatg
    40621 aagggaaata cctgatgatc cacggcgatt gtgtttccat cggctgctac gcgatgacca
    40681 atcagggcat tgacgagatt ttccagttcg ttactggcgc actggtattt ggtcagccga
    40741 gcgtgcaagt gagcatttac ccgttccgca tgaccgacgc taatatgaag cgccacaaat
    40801 attccaactt taaggacttc tgggagcaac tgaagccggg ttacgactac tttgagcaaa
    40861 cccgtaagcc accgaccgtt tctgtggtca atggtcgtta cgtggtcagc aagccgttaa
    40921 gccacgaagt tgtgcaacca cagctggcat caaactacac gctccccgag gcaaaataag
    40981 cgccattcgc ctggcataat tttttgccag gtttcattgc ccgtcagcgg ctgtgtcgca
    41041 atcaccgtga ccacatcatt cggtgtggtc tgcgagctga agtcgatttc cacatcctga
    41101 tccagcaacg ttgccacgcc aaacggcgcg cggcgggtga tccagtgtaa attagtcgag
    41161 caatacgcca ttacatagcg cccgtcagaa agcagcatgt tgaaaacgcc cttctgccgc
    41221 agttcatccg ccagtgaggc gatatattta aataccgccg ccatgttgcc cggtgtgcgc
    41281 gggtaacgct gcgttaattt atgcaggagc cagcaaaagg ctttttcgct gtcggtttcg
    41341 cctaccgggc ggaagttgcc ggtttccagt gatttgtagc ccgtcagttg tccgttatgg
    41401 gcataagtcc agttacgccc ccataactcg cgggtaaatg ggtgagtatt ttccagcgcc
    41461 acctcgcccc gattagcctg gcgaatatga gccaccaccg aacaggattt tatcgggtag
    41521 tcctggacaa gtttggcgat gggggaatta aagctgggtt gtggatcttt aaatgtgcga
    41581 cagcctttac cttcgtaaaa ggtaatgccc cagccatctt tatgtggccc ggttccacca
    41641 ccacgctgta caagcccggt gaaactaaag cagatatcgg tagggacgtt ggcgctcatc
    41701 ccgagcaatt cgcacatggg taacctccac aacagcggga gcgcatggcc cccggcgaag
    41761 attgatttaa gaattttcag gtcggatgcg gcgtgaacgc attattcggc ctacggttcg
    41821 cgtacagatt tgtaggcatg ataagacgcg tcagcgtcgc atcaggcatc ggtgcacaat
    41881 tgccggatgc ggcgtaaacg tcttatccgg cctacgccag acttacttaa ccatctcttt
    41941 ttcaatcaac tggatcagga tatggatcac tttaatgtga atctcctgaa tgcggtcggc
    42001 ataaccaaag tgcggtacgc gaatttcgat atccgccgtg ccagccattt tgccgccgtc
    42061 tttaccggtc agggtgatca ctttcattcc cttctcacgc gccgctgcga tcgctttgat
    42121 cacgtttgca gagttaccgg aggtggagat ccccagcagt acatcgcctt cgcgacctac
    42181 cgcttcaacg taacgggaga aaatatcatt gaaaccgaaa tcgttaccga cgcatgaaat
    42241 atgactaacg tcagaaatag caatcgccgg gtagcccgga cggttttcac ggtagcgacc
    42301 ggtcaactct tcggcaaagt gcatagcgtc gcaatgggaa ccgccgttgc cgcaggaaag
    42361 cactttgcca ccggctttaa agctgtctgc taacaggacc gccgcgcgct gaatggcgtg
    42421 aatattggcg tcatctttta aaaagttagc cagcgtttcc gccgcttcgt tcagttcgtt
    42481 acgaataaga tcctggtaca tgaggatatc cttcagcata aatgtaatag acaaaatgca
    42541 gtgtaccgga taccgccaaa agcgagaagt acgggcaggt gctatgacca ggactttttg
    42601 acctgaagtg cggataaaaa cagcaacaat gtgagctttg ttgtaattat attgtaaaca
    42661 cattgctaaa tgtttttaca tccactacaa ccatatcatc acaagtggtc agacctccta
    42721 caagtaaggg gcttttcgtt atgatgattt tgagtattct cgctacggtt gtcctgctcg
    42781 gcgcgttgtt ctatcaccgc gtgagcttat ttatcagcag tctgattttg ctcgcctgga
    42841 cagccgccct cggcgttgct ggtctgtggt cggcgtgggt actggtgcct ctggccatta
    42901 tcctcgtgcc atttaacttt gcgcctatgc gtaagtcgat gatttccgcg ccggtatttc
    42961 gcggtttccg taaggtgatg ccgccgatgt cgcgcactga gaaagaagcg attgatgcgg
    43021 gcaccacctg gtgggagggc gacttgttcc agggcaagcc ggactggaaa aagctgcata
    43081 actatccgca gccgcgcctg accgcagaag agcaagcgtt tctcgacggt ccggtagaag
    43141 aagcctgccg gatggcgaat gatttccaga tcacccatga gctggctgat ttgccgccgg
    43201 agttgtgggc gtaccttaaa gagcatcgtt tcttcgcgat gatcatcaaa aaagagtacg
    43261 gcgggctgga gttctcggct tatgcccagt ctcgcgtgct gcaaaaactc tccggcgtga
    43321 gcgggatcct ggcgattacc gtcggcgtgc cgaactcatt aggcccgggc gagctgctgc
    43381 aacattacgg cactgacgag cagaaaaatc actatctgcc gcgtctggcg cgtggtcagg
    43441 agatcccttg ctttgcactg accagcccgg aagcgggttc cgatgcaggc gctatcccgg
    43501 ataccgggat tgtctgcatg ggcgaatggc agggccagca ggtgctgggg atgcgtctga
    43561 cctggaacaa acgctacatt acgctggcac cgattgcgac cgtgcttggg ctggcgttta
    43621 aactctccga cccggagaaa ttgctgggtg gcgcagaaga tttaggtatt acctgtgcgc
    43681 tgatcccaac caccacgccg ggcgtggaaa ttggtcgtcg ccacttcccg ctgaacgtgc
    43741 cgttccagaa tggaccgacg cgcggtaaag atgtcttcgt accgatcgat tacattatcg
    43801 gcgggccgaa aatggccggg caaggctggc ggatgctggt ggagtgcctc tcggtaggcc
    43861 gcggcatcac cctgccttcc aactcaaccg gcggcgtgaa atcagtagcg ctggcaacgg
    43921 gtgcgtatgc gcacattcgt cgtcagttca aaatctctat tggtaagatg gaagggattg
    43981 aagagccgct ggcgcgtatt gccgggaacg cttatgtaat ggatgctgcg gcatcgctga
    44041 ttacctacgg cattatgctc ggcgaaaaac ctgccgtgct gtcggctatc gttaagtatc
    44101 actgtactca ccgcgggcag cagtcgatta ttgatgcgat ggatatcact ggtggcaaag
    44161 gcattatgct cgggcaaagc aacttcctgg cgcgtgctta ccagggcaca ccgattgcca
    44221 tcaccgttga aggggctaac attctgaccc gcagcatgat gatcttcgga caaggagcga
    44281 ttcgttgcca tccgtacgtg ctggaagaga tggaagcggc gaagaacaat gacgtcaacg
    44341 cgttcgataa actgttgttc aaacatatcg gtcacgtcgg tagcaacaaa gttcgcagct
    44401 tctggctggg cctgacgcgc ggtttaacca gcagcacgcc aaccggcgat gccactaaac
    44461 gctactatca gcacctgaac cgcctgagcg ccaacctcgc cctgctttct gatgtctcga
    44521 tggcagtgct gggcggcagc ctgaaacgtc gtgagcgtat ctcggcacgt ctgggtgata
    44581 ttttaagcca gctttacctc gcctctgccg ttctgaagcg ttatgacgac gaaggccgta
    44641 atgaagccga cctgccgctg gtgcactggg gcgtacagga tgcgctgtat caggcagaac
    44701 aggcgatgga cgatttactg caaaacttcc cgaaccgcgt ggttgccggg ctgctgaatg
    44761 tggtgatctt cccgaccgga cgtcattatc tggcaccgtc tgacaagctg gatcataagg
    44821 tggcgaagat tttacaagtg ccgaacgcca ctcgctcacg tattggtcgg ggtcagtacc
    44881 tgacgccgag cgagcataat ccggttggtt tactggaaga ggcgttggtg gatgtgattg
    44941 ccgccgaccc gattcatcag cggatctgta aagagctggg taaaaatctg ccatttaccc
    45001 gtctggatga actggcgcac aacgcgctgg cgaaggggct gattgataaa gatgaagccg
    45061 ctattctggt gaaagcggaa gaaagccgtc tgtgcagtat taacgttgat gactttgatc
    45121 cggaagagct ggcgacgaag ccggtaaagt tgccggagaa agtgcggaaa gttgaagccg
    45181 cgtaattgaa taacggagcc gaaaggctcc gtttctttat ccgctaatta tttaaaatta
    45241 aagccatccg gatggttttc caggctgccg gtcaacgccg cgaacagcac cgttttacca
    45301 tcaatcgaaa gcgcatcgtt cacattcagc caggtgagtt tctcttgcga cgttttctca
    45361 tcaatagtcg agaacagccc cgtcatctga ttagatttct cggaccacat cacagcgata
    45421 cgttgcgagc cacagtcatg cggtttgcac gcgctcatca cctgatacgt ctcatctccc
    45481 aacgttacgg tttgtgcggg agtataagta ccgcctttca tcacccatgc aggtagctta
    45541 tgcccttgca ccatctgatt aaacgcagct ttggtggttt cgccctttgc caggctgcta
    45601 atggttaaat catcctgcgc cattgcactg gtggcgatga ccagagcggc gactgtcgtt
    45661 attgccttaa acatcattcc tcccgagctt atcctgccca tgttattaac ctcctaaagc
    45721 ctggcgataa cacgataatt tgcaaatcag atatttccaa tatcagcatt tgttatcaat
    45781 ttattctgcg ctaaggcaac tgcctgataa ggtgttaaag ttataaaacc cttcgcataa
    45841 aggagttgta caccgtgcct ggtttgaaga ttacgctttt gcagcaacca ctggtgtgga
    45901 tggatggccc tgccaacctg cgtcattttg atcgtcaact ggaaggtatt accgggcgcg
    45961 atgtgatcgt tctaccggag atgtttacca gcggctttgc catggaagcg gcagcttcgt
    46021 cgctagcaca aaatgacgta gtgaactgga tgacagctaa agcgcagcag tgcaatgcgc
    46081 tgattgcagg cagtgttgca ttacaaacgg agtctggttc ggttaaccgc tttttgctgg
    46141 ttgagccggg cggcacggta catttttatg ataagcgtca tctgttccgc atggcagatg
    46201 agcatctgca ttataaagcg ggcaatgcgc gagtgattgt ggaatggcgc ggctggcgta
    46261 ttttgccgct ggtgtgctac gacttacgtt ttcctgtgtg gtcgcgcaat ctcaacgatt
    46321 atgacctcgc catctacgtt gccaactggc ctgctccgcg ctctctgcac tggcaggcat
    46381 tgctgacggc ccgcgcgatt gagaatcagg cgtatgtggc gggatgcaat cgcgtcggca
    46441 gcgatggcaa cggctgccat tatcgcggtg acagccgggt gattaacccg cagggcgaga
    46501 ttatcgctac tgccgacgcg catcaggcaa cgcgcattga tgcggagctg tcgatggtgg
    46561 cattgcggga atatagagaa aagtttccgg catggcagga tgcggatgag tttaggttgc
    46621 ggtgagtggg ggttgtgttt aaggctgggg gagagtcggg gtaagactac gaaagcccgc
    46681 tctccgcaag gactgacgcc agatagtttc tgtccatagc tgcttttcgc atcttacgtc
    46741 ttaaccctgc cttgaatacc ttatcattcg tcaaaatatt aatagcgatg tgccgtatcc
    46801 ctgaaaataa ttctgcggcg tttcctcttc ttattttgca gtcgtcttca ttcattacca
    46861 cgtccagacg ccagtgcagc ttattctcca cgtgccagtg gtttcggatt gctgtggcga
    46921 acttttctgc ggttaaatca gcagaactga tataatatct gaccgtcatt tctggctctt
    46981 tcttttgttc tgctattatt gaccgaaagg agactgccac gcataatttc ttcagtcctt
    47041 tccattcaaa cgtgaaatca ataagttcat cagggacatc gcaaacaata tgaagacgga
    47101 tttcttctct gccgtgactc ttttcactca ttgcgtaact gtcatgctct ggattattta
    47161 attctttcag cggaaatttt tcctcaaagg ctttatttag ccgcccctgg tttcctttta
    47221 ccgcgaataa ataatcacct ccctgttttt gtatcttctc tgcaatatct ttctggcaac
    47281 ccatcgcatc agttgtgatg atttttcctt taatatccag catgttaaga agttctggga
    47341 tagctgtaat ctcattagat ttctcatccg tcctgatctg tccgatgacc agactgtgca
    47401 ttgttgagaa cgcactaatg acatgaatcg ctcccctg
//
LOCUS       DAJOWB010000023        47175 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJOWB010000023 DAJOWB010000000
VERSION     DAJOWB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.023"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(93..1343)
                     /locus_tag="OHO85_001461"
     CDS             complement(93..1343)
                     /locus_tag="OHO85_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCQ2670855.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(1437..2375)
                     /gene="psuG"
                     /locus_tag="OHO85_001462"
     CDS             complement(1437..2375)
                     /gene="psuG"
                     /locus_tag="OHO85_001462"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCQ2670856.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEHTINAAIDQAVAEAEAQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(2363..3304)
                     /locus_tag="OHO85_001463"
     CDS             complement(2363..3304)
                     /locus_tag="OHO85_001463"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCQ2670857.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(3728..5419)
                     /gene="fruA"
                     /locus_tag="OHO85_001464"
     CDS             complement(3728..5419)
                     /gene="fruA"
                     /locus_tag="OHO85_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCQ2670858.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(5436..6374)
                     /gene="fruK"
                     /locus_tag="OHO85_001465"
     CDS             complement(5436..6374)
                     /gene="fruK"
                     /locus_tag="OHO85_001465"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCQ2670859.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(6374..7504)
                     /gene="fruB"
                     /locus_tag="OHO85_001466"
     CDS             complement(6374..7504)
                     /gene="fruB"
                     /locus_tag="OHO85_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCQ2670860.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGGDAEQALKAIGDAIAAGLGEGA"
     gene            7872..9053
                     /gene="setB"
                     /locus_tag="OHO85_001467"
     CDS             7872..9053
                     /gene="setB"
                     /locus_tag="OHO85_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCQ2670861.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLLAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(9050..9304)
                     /locus_tag="OHO85_001468"
     CDS             complement(9050..9304)
                     /locus_tag="OHO85_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCQ2670862.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            9459..10031
                     /gene="yeiP"
                     /locus_tag="OHO85_001469"
     CDS             9459..10031
                     /gene="yeiP"
                     /locus_tag="OHO85_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCQ2670863.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            10254..11720
                     /locus_tag="OHO85_001470"
     CDS             10254..11720
                     /locus_tag="OHO85_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCQ2670864.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            11838..12824
                     /gene="yeiR"
                     /locus_tag="OHO85_001471"
     CDS             11838..12824
                     /gene="yeiR"
                     /locus_tag="OHO85_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCQ2670865.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            12863..13576
                     /gene="lpxT"
                     /locus_tag="OHO85_001472"
     CDS             12863..13576
                     /gene="lpxT"
                     /locus_tag="OHO85_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCQ2670866.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            13988..14554
                     /gene="mepS"
                     /locus_tag="OHO85_001473"
     CDS             13988..14554
                     /gene="mepS"
                     /locus_tag="OHO85_001473"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCQ2670867.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            14735..16291
                     /locus_tag="OHO85_001474"
     CDS             14735..16291
                     /locus_tag="OHO85_001474"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCQ2670868.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHRHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            16373..18187
                     /locus_tag="OHO85_001475"
     CDS             16373..18187
                     /locus_tag="OHO85_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCQ2670869.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            18188..19282
                     /locus_tag="OHO85_001476"
     CDS             18188..19282
                     /locus_tag="OHO85_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCQ2670870.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            19282..20307
                     /locus_tag="OHO85_001477"
     CDS             19282..20307
                     /locus_tag="OHO85_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2670871.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVMRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            20309..21898
                     /gene="yejF"
                     /locus_tag="OHO85_001478"
     CDS             20309..21898
                     /gene="yejF"
                     /locus_tag="OHO85_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCQ2670872.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(21902..22246)
                     /locus_tag="OHO85_001479"
     CDS             complement(21902..22246)
                     /locus_tag="OHO85_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670873.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(22579..23769)
                     /gene="bcr"
                     /locus_tag="OHO85_001480"
     CDS             complement(22579..23769)
                     /gene="bcr"
                     /locus_tag="OHO85_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCQ2670874.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(23797..24492)
                     /gene="rsuA"
                     /locus_tag="OHO85_001481"
     CDS             complement(23797..24492)
                     /gene="rsuA"
                     /locus_tag="OHO85_001481"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCQ2670875.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            24642..26402
                     /locus_tag="OHO85_001482"
     CDS             24642..26402
                     /locus_tag="OHO85_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCQ2670876.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            26527..26811
                     /gene="rplY"
                     /locus_tag="OHO85_001483"
     CDS             26527..26811
                     /gene="rplY"
                     /locus_tag="OHO85_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCQ2670877.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(26950..27957)
                     /gene="yejK"
                     /locus_tag="OHO85_001484"
     CDS             complement(26950..27957)
                     /gene="yejK"
                     /locus_tag="OHO85_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCQ2670878.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGAREGLNAKAQNRGLLQAVDDFTAQAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            28139..28366
                     /locus_tag="OHO85_001485"
     CDS             28139..28366
                     /locus_tag="OHO85_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCQ2670879.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            28386..30146
                     /gene="pbgA"
                     /locus_tag="OHO85_001486"
     CDS             28386..30146
                     /gene="pbgA"
                     /locus_tag="OHO85_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCQ2670880.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            30221..30297
                     /locus_tag="OHO85_001487"
     tRNA            30221..30297
                     /locus_tag="OHO85_001487"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30255..30257,aa:Pro,seq:ggg)
     gene            complement(30399..32768)
                     /locus_tag="OHO85_001488"
     CDS             complement(30399..32768)
                     /locus_tag="OHO85_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCQ2670881.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVVLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGISSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGAANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGG
                     LAIAVDQKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYELDDGSGLIVMEDTQAI
                     DTILDEHATMQSLGKDTGTRVQANAVYDLGRSDQNGSITYSSKAISENMVINNGRANV
                     WAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGASFRTHGAVDTSKADVSL
                     ENSVWTIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRSSVTASAENFITLTTNT
                     LSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASPAAGDSLTLVTTGGGDA
                     AFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTTDVLNMAAAQPLVFDAE
                     LDTVRERLGSVKGVSYDTAMWSSAINTRNNVTTDAGAGFEQTLTGLTLGIDSRFSREE
                     SSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNGAYVDGVVKVDRFANTI
                     HGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAFTGFTTDGQDYTLSNGM
                     RADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEYADSNQVKVNDDGKFNN
                     DVAGARGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQAGVVWTF"
     gene            33088..33735
                     /gene="narP"
                     /locus_tag="OHO85_001489"
     CDS             33088..33735
                     /gene="narP"
                     /locus_tag="OHO85_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCQ2670882.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(33770..34822)
                     /gene="ccmH"
                     /locus_tag="OHO85_001490"
     CDS             complement(33770..34822)
                     /gene="ccmH"
                     /locus_tag="OHO85_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCQ2670883.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(34819..35376)
                     /gene="dsbE"
                     /locus_tag="OHO85_001491"
     CDS             complement(34819..35376)
                     /gene="dsbE"
                     /locus_tag="OHO85_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCQ2670884.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(35373..37316)
                     /gene="ccmF"
                     /locus_tag="OHO85_001492"
     CDS             complement(35373..37316)
                     /gene="ccmF"
                     /locus_tag="OHO85_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCQ2670885.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(37313..37792)
                     /gene="ccmE"
                     /locus_tag="OHO85_001493"
     CDS             complement(37313..37792)
                     /gene="ccmE"
                     /locus_tag="OHO85_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCQ2670886.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(37789..37998)
                     /gene="ccmD"
                     /locus_tag="OHO85_001494"
     CDS             complement(37789..37998)
                     /gene="ccmD"
                     /locus_tag="OHO85_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCQ2670887.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(37995..38732)
                     /gene="ccmC"
                     /locus_tag="OHO85_001495"
     CDS             complement(37995..38732)
                     /gene="ccmC"
                     /locus_tag="OHO85_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCQ2670888.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(38774..39436)
                     /gene="ccmB"
                     /locus_tag="OHO85_001496"
     CDS             complement(38774..39436)
                     /gene="ccmB"
                     /locus_tag="OHO85_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCQ2670889.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(39433..40050)
                     /gene="ccmA"
                     /locus_tag="OHO85_001497"
     CDS             complement(39433..40050)
                     /gene="ccmA"
                     /locus_tag="OHO85_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCQ2670890.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(40069..40671)
                     /gene="napC"
                     /locus_tag="OHO85_001498"
     CDS             complement(40069..40671)
                     /gene="napC"
                     /locus_tag="OHO85_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCQ2670891.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(40681..41130)
                     /gene="napB"
                     /locus_tag="OHO85_001499"
     CDS             complement(40681..41130)
                     /gene="napB"
                     /locus_tag="OHO85_001499"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCQ2670892.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(41127..41990)
                     /gene="napH"
                     /locus_tag="OHO85_001500"
     CDS             complement(41127..41990)
                     /gene="napH"
                     /locus_tag="OHO85_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCQ2670893.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(41977..42672)
                     /gene="napG"
                     /locus_tag="OHO85_001501"
     CDS             complement(41977..42672)
                     /gene="napG"
                     /locus_tag="OHO85_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCQ2670894.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(42679..45165)
                     /gene="napA"
                     /locus_tag="OHO85_001502"
     CDS             complement(42679..45165)
                     /gene="napA"
                     /locus_tag="OHO85_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCQ2670895.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(45162..45425)
                     /gene="napD"
                     /locus_tag="OHO85_001503"
     CDS             complement(45162..45425)
                     /gene="napD"
                     /locus_tag="OHO85_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCQ2670896.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(45415..45909)
                     /gene="napF"
                     /locus_tag="OHO85_001504"
     CDS             complement(45415..45909)
                     /gene="napF"
                     /locus_tag="OHO85_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCQ2670897.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            46018..46182
                     /locus_tag="OHO85_001505"
     CDS             46018..46182
                     /locus_tag="OHO85_001505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670898.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCPLIRI"
     gene            46317..46805
                     /gene="eco"
                     /locus_tag="OHO85_001506"
     CDS             46317..46805
                     /gene="eco"
                     /locus_tag="OHO85_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCQ2670899.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
BASE COUNT        11472 a        12837 c        11645 g        11221 t
ORIGIN      
        1 cagccgcagc acatgcaact tgaagtatga cgagtatagt acgcgattgc cggatgcaac
       61 gcttaataag cgagtacatc cggcaatgaa actcaagcta aaccaataaa gaacccggca
      121 atggtcgcgc tcatcaggtt ggaaagcgtc gccgccgcca gcgcccgtaa accaagctgg
      181 gcgatttccg gcgcgcgttt tggcgaaata gccgaaaacg ctccaacgac aacaccgata
      241 gaaccaaagt ttgcaaaccc acagagggcg aacgagataa tggcaatggt cttgacatcc
      301 agcgtgccgc tcgtttgcag atagggcgag aaattgagat aagcaacaaa ctcattaatc
      361 gccagtttct gcccaatcaa acttccggca agatttgcat cactccagtc cacccccata
      421 atccatgcca acggggccag cacatagccg aaaatgcttt ccagagagac attggcaaaa
      481 ccaaaccagc cgccaactcc accaataatg ccgttgatca gcgcaatgat cgccacaaat
      541 gccattacca ccgtcgccac accggcggcg attttcagcc cggtcattgc accgttcgcc
      601 gcagcttcaa taatgctttt tggcggtgtt tcgctaaacg acagattttc aaacgtaact
      661 tgcgaaggtt ccgtcgccgg actaagaata cgtgcaaaca ggataccgcc aggaatagcc
      721 atcagcgaag ccgctaacag gtagtcaatt ggtacgccca ttccggcata accaatcatc
      781 atcgaaccag caatggacgc catcccgcta caaattgcgg taaacaactc gttgcgattc
      841 atgcgatcga taaacggttt aacgatcgcc gggatctcat tttgcccgag gaaaatagtt
      901 gtgactgcga caaaagattc gattttgctg atgttaagag ctttctggaa aatgctgcca
      961 aggatgcgaa tcagcagccc catcacgcca atgtagtaca gcagactgat gagcgcagta
     1021 acgaaaataa tcgccggaag tacgcgaaag gcgaagataa aacccgcacc gtcaaacagg
     1081 acatccattt tcggcccaac cagcgaacca aaaatgaagg cgctaccggc atcactgtaa
     1141 gacatcactt tatgaacgcc taatgccgcc tgttctactg cccactttcc cggtgggaag
     1201 tagagcatga tgccaccaat agcgatttgc agcagcagtg cggctccaac cgtgcgcaaa
     1261 ctgatgctct ttttattcac tgacaacaga aatgctattg ccagtaacac caccatcccc
     1321 acaacacttc tcattatatc cataatgatt ttcccttcat gccagtaaac ccggcgtcag
     1381 cgccaggttt tggtatgctt gatgagtacg ggcgacggct ttctgcccgt cagaaattaa
     1441 cccgcgagac gctggtattc tttggcaatt tcactcgcca gaatggcgtt gttgaacacc
     1501 agctggatat tggatttcag gctgtcaccg ccggtcagct cagcaacgcg cgccagcagg
     1561 aacggtgtac tttctttacc aataacgccc tgcgcttcag cttcagctac cgcctgatcg
     1621 atcgccgcat tgatagtgtg ttccggcatc gcaaactgtt ccgggatcgg gttcgctacc
     1681 acgaggccac cgttcagccc gctttgccac ttcaccgcca ttgcacgggc aatttcactg
     1741 gcgctgtcga gacgaatgct gacgtcaaac gggctggtac ggcagaaaaa cgcaggcagc
     1801 gcttttgtct gatagccaat taacggcaca ccgaaggttt ctaaatattc tgttgttaat
     1861 cctaaatcga gaatagattt cgccccggca caaacaacgg tgacattagt atttgccagt
     1921 tcttgcaaat cggcagaaat atcgaaggta tgttccgccc cgcgatgcac accaccaatt
     1981 cccccggtgg caaatacttt aattccggca agcgccgcaa taatcatcgt tgaagccaca
     2041 gtggttgcgc cattttttcc ggcggcaaca acaaaaggta aatcgcgacg actaactttg
     2101 gtcacgttat gcccttcacg acccagtaat tcaatttctt ctttgcttaa acccactttc
     2161 atcacgccgc caataatggc gatagtggca ggtactgcgc cctgtttacg aatagtttct
     2221 tctacttcaa ttgcggtctg ggcattttgt gggaacggca tcccgtgaga aataatggtc
     2281 gattccagcg ccacaaccgg ttttttgttt tttaaagcgt cctgcacttc cggggaaatt
     2341 tgtaataatt caggggaaat ttttaattca gacattctgc gttctccact aacgatataa
     2401 cgttggcaat cgataaatcg gggttattgg tgtattcaca ggagagcgcc attgacgaac
     2461 atccctgtgc gaaacgaaca gattcggcaa acggcattcc gtctacccaa cacgaagcaa
     2521 gtcccgccat catggcatcg cccgctccgg taacattaat aacattggtt ttgatcggcg
     2581 cagaccagcc attttcaccg ctgatatcgc tgtaataaac gccgtcgccg cccatgctca
     2641 ataccagtcg gttcaggcca tgttgatgga accaggcagc aacttttgcc acatcgtcac
     2701 gccctgacag cgcaatccca ctcagggttt ccgcttcaag gcggtttggc ttgagagtgt
     2761 ggatctgatt tagacggtcg cgcactttga cacatttcca cgctgaaacc ggatcgacaa
     2821 atacgggtac gttggcggca ttatccagaa tccatgccag tgcctcttca ctgatattac
     2881 agtctgcgac aatgaccttt gccctctgaa taaattcacg gtgctgtgcg agatattcag
     2941 ctgtaatagc gttgctaata ttcatgtcat ttatagcaac cagcatttca ccggtattat
     3001 cgagtaatga taaatagctt gacgtatttt ctcctggcac aatcaggcat ttatcgacat
     3061 aaacgccaga ttgattggtt tgcgttagca gcgattgacc ataaaagtca ctgcctacgg
     3121 cgctcagtag ccaggctttg ttccccagca acgccaggtt ttgtgcaata ttgcgcccga
     3181 ctccaccagg cgtaaatttt attttacctg gatttgaatc cgcataattt aatgattcat
     3241 gtgaatatcc ggcgacatca atattcgccg agcctataat tacgacataa tccttttcgc
     3301 gcatagcacg tcctctggta aaaaattacc aaccctgtca atccacaaga atatagatac
     3361 tgcaaagacg gtttaaacat gtgttcacat ttaaacatgt gctcatagta tgcatgtttt
     3421 acgtaaagta aattaccgcc agagaggcta aatgggcaga tttgagcagg ctcacaaaat
     3481 gcgattaaat gcctgaatta caaaagcgag gaatttgtga cgggatgcac agaacaggaa
     3541 caataaaaaa gggcagaaaa taatctgccc ttgaggaaat aaccttagtg tttgatgaac
     3601 gtttcaagtt tctactgaaa gataaaacca tttaaattca taaaattaca ttcacaagct
     3661 ggtcattccc tttgcgctca tccaatgata cagggcagga aatttctgcc ctgtaacaca
     3721 ccttttatta cgctgctttc gctactgcgt ccacttccgg acgtttcagg aaggcatagg
     3781 ccaaacccgc caccagcgta ccggcaataa ttgctaccag gtaacccagt accggcgtaa
     3841 tagcgccagg gatcagcaga acaaacagac caccgtgtgg tgccatcagt ttcgcaccaa
     3901 tcgccattga gattgcgcca gtcagcgcac cacccacgat acagcacggt agcacacgca
     3961 tcggatcacg agcagcaaac ggaattgcac cttccgaaat gaagcacagt cccaatacca
     4021 gagcggcttt gccaccttcc tgctgcgctt tgtcgaattt gcgacgcgcc accattgttg
     4081 ccagacccat tgccagcggt ggcaccatac ctgccgccat aatcgccgcc atcgggccat
     4141 aggtttgagt actcagcaga cccacaccga atgcgtacgc tgctttgttt accggaccgc
     4201 ccatgtcagt acacatcatg ccaccgagga tcgcccccag cagaaccgca ttcgcagtcc
     4261 ccatggtctg cagccagtga gtcagccctt cgagaatgcc agcaaccggt ttaccgatca
     4321 ggtagatcat cgccagaccg accaccagac tggaaattag cggaatgatc aggattggtt
     4381 tcagcgcctc catactctgt ggcagtttca gttgcgtact gattaacttc gcaatgtaac
     4441 cagccaggaa gcccgcaata ataccgccaa tgaagccaga accggtgctg acagccagca
     4501 taccgccaat cagccccggc gtgaggcccg gacgatcggc aatggagaag gcaatataac
     4561 ctgccagtac cggcaccatc agcgcaaagg ctgaaccacc gccaatctgc atcagcgccg
     4621 ctgccaacgt acccggctct ttaaacgctt cgataccaaa agcaaaagaa agcgcgatac
     4681 acagaccacc tgcaacaacc atcggcagca tgtaagagac gcccgtcagc aagtgacggt
     4741 aagcgcctgc actctctttc ttaccttcag tggtcgctgt ttgagctttg cccgctggtt
     4801 catacggcgt tgcttcagca accgctttat ccagttcctg cgcggttttc ttcagcgcca
     4861 gaccggtaga ggtacgatac atcggtttac cagcaaattt cgccagatcc acttcgatat
     4921 ctgccgccac aatcaccaga tccgctgcgg ctacttcctc gggagtgatt gcattacccg
     4981 cgccaacaga accacgggtt tcaactttca cccaccagcc acgttttttc gcttcggttt
     5041 caatggcttc agccgccata aaggtgtgtg ctacgccagt cgggcaagca gtcaccgcaa
     5101 ctacgcgttt cggaccgctg gcggcaaccg gtgctgtcgc agtgaccggc gcagtgtaag
     5161 gtttcgcatg gcctttggct tcactcagga acagctcagg gtgcgcaact gcccgggaaa
     5221 tatcacccag ccagacattt ttaccgttca gcgcgctgtc gttcgggatg gaatcaccga
     5281 gaacaatcgc catttcagcg tcgttcggat tgtcgatgat ttccagtttt gcttttcgcg
     5341 ccgccgcgcc cagcagggtc ttcgccatat aggcgcgtgc ctgaccgaga ttagcgtcaa
     5401 taatcagcag cgttttcatt atgcctctcc tgctgtcagt taaaaggttg taagtcgacg
     5461 cgcgccatca ttgcggccaa ctgcggacga tcggtaatac ccacattgct ttgacttacc
     5521 gccagggctg caacagctgt cgccagacgc agtgtgtgtt cactggattc acgcatcagc
     5581 aagccataaa tcaggccacc aaccatagaa tcccctgcgc caacggtgct tacgacatcg
     5641 actgacggtg gtttggcgat ccattcgccg gaggcattaa cccaaagcgc gccttcggca
     5701 cccagtgaaa taacaacatg cgcgatgcct tgttcacgta gcgcatgtgc agcttcaatc
     5761 acatctttca tttcaggcag tttacggcct gcccagattt ccagctcgcg gcggttaggt
     5821 ttcaccagcc acggtgccgc tttcaaacct gctactaacg cttcacggct actatcaaag
     5881 ataatgcaag gacactgact acgcaggcga gtcatccagt cggtgaacgc ttccgggctg
     5941 acgcctgacg gtaagcttcc gctgacacag accatatcga actgaccgag ccagctcaga
     6001 gaatcagtca caaagcgttc ccagtcggcg ggggtgactt caaaacccga gaagttgaag
     6061 tcggtcactt caccgtcttt ttccgtcagc ttaacgttaa ttcgggtgcg cccctgtaca
     6121 acctggaaac ggttggcaat gcccagctcg ctgaacagtt gctgaaaacc atcctgattg
     6181 tctttaccga ggaagccgcc aacggtgaca tcaattccca ggtcttttaa tactttggcc
     6241 acgttgatgc ctttacccgc cgcatgcaga ccggtggttt tcaccaggtt cacttcgccg
     6301 cgttcaattt ccgggcagaa accaacaagg tcataagccg gattaagggt gatagtagca
     6361 acacgtctgc tcattatgcg ccctccccaa gaccagcagc gatagcgtcg ccaatggctt
     6421 tcagcgcctg ttcagcatct ccaccctggg cggtaaagcg taggcgatga cctttcttaa
     6481 cgccaagtgc cacaactttc atcagactac gtccgtttgc cggtttgccg gtgccatcaa
     6541 ggtttgtcac ggtaatatca ctgttaaatt gtttaatggt attgaccagc atggtacctg
     6601 gacgagcatg caggccgtgt tcattgcgca ccacaaactc cgcgcttaac acgtcgtcgg
     6661 tcggcgcatc atcgctggtc agcagtgcca gcaacgttgc cgcatccgct ttcagcaagc
     6721 ggtcagcttt attgtcgagc aacaaatcag cgagacgctt aagaaccgcg atgggctgat
     6781 cgtcattcat cgctacactc accagcatgg ctgccgtttc gccgtccaca tcaaaagcat
     6841 ttgccgcacg gcttaccgca atcgcgctac gcagattgcc ttcggcgcta tcgctcagcc
     6901 agatgccctg tccgaggttc agcggttgtt cattgatggc tttggtgacg aaagtggcgt
     6961 caactgcccc cgcctctttc agacgcgcag cgttcagcgc ctgaagagtc agcagatcgc
     7021 tggcgacgat atccagtgtc agcatttcgt tgtcgagctt cagctgctca ctctgctttt
     7081 cgcccatcag caatgcgcga agttcttctg ctgttgttgc tgacttcagt tgttcagcaa
     7141 cggaatcatc actcagtacg tgggtcagct ggcgtagcag gcccagatgt tcatccgagc
     7201 tggcagcaat accgatcgcc acgtacgcta cctgaccgtc accccaggtg acgccttccg
     7261 ggaactgaaa tacctgaacg ccggttttca gcacctgatc gcgggtgtcg gtggtgccgt
     7321 gtggaatagc aataccattg ccgaggaacg ttgaggtttg ctgttcgcgc gccagcatgc
     7381 cattgacgta gccttctgct acgttaccgg cctgcaccag cgccgcagcg acctggcgaa
     7441 tcgcctcttc tttgtctccg gccttttcgc ccggatggat gtcctgtacg gataactgga
     7501 acatagttct cctctcttgc tgaattgaaa cgattcagcc tctatgagaa aaaaagcgcc
     7561 aacctggctt agggttaaag acaagatcgc gctgaaacgt ttcaagaaag cataatactt
     7621 ctgattcagc acgcaaggaa agccgtaaag ttatgagcaa aagtttgatg tgctgcacaa
     7681 ttttttgtca tttttcctaa ttgctgatgg gaaaatccgt tatcagcaat ttcatttcag
     7741 cagcgtcagc agccaatcag caacagtgaa atggcatttt gattattcgt ggcgcttttg
     7801 atttcgctgt ctgtttattt tctgacaagc agcgtaaact ccgcgtcttc ctcttccagt
     7861 gatcgaccag catgcataac tcccccgcag tctccagcgc gaaatcgttt gacctgacct
     7921 cgacggcgtt tttaatcgtt gcctttctca ccggtattgc gggcgctctg caaaccccga
     7981 cactcagtat ttttcttact gatgaagtac atgcccgtcc ggcgatggtg ggattcttct
     8041 ttaccggcag cgctgtcatt gggattctgg taagtcagtt tctcgccggg cgctctgata
     8101 agcgcggcga tcgcaaatcg ctgattgtct tttgctgcct gttaggcgtg ctggcctgca
     8161 ccctttttgc ctggaatcgc aactactttg ttttgctatt cgttggcgtc tttcttagca
     8221 gctttggctc gaccgctaac ccgcaaatgt ttgcccttgc ccgtgaacat gccgacaaaa
     8281 ccggacgtga agcggtgatg ttcagctctt ttttacgcgc tcaggtttca ctggcatggg
     8341 tcattggccc accgctggct tatgccttag cgatgggttt cagctttacg gtaatgtatc
     8401 tgagcgcagc ggtagcattt attgtttgcg gcgtgatggt gtggctgttt ttaccgtcga
     8461 tgcaaaaaga gcttccgctg gcgaccggca cggttgaagc gccgcgccgt aaccgtcgcg
     8521 atacgctgct gctgtttgtc atttgtacat tgatgtgggg ctcgaacagc ctgtacatca
     8581 tcaacatgcc gctatttatt atcaacgaac tgcatcttcc cgagaaactg gccggtgtga
     8641 tgatggggac cgccgccggg ctggaaatcc cgacgatgtt gcttgccgga tatttcgcca
     8701 aacgtctggg taagcgtttc ttaatgcgcg ttgctgccgt gggtggcgtc tgtttttacg
     8761 caggaatgct gatggcgcat tcacctgtca ttctgttggg cttgcagctg ctaaatgcta
     8821 tttttattgg cattctgggc ggcatcggga tgctctattt tcaggatctg atgcccggtc
     8881 aggcgggttc agccaccacg ctctatacca acacgtcgcg cgtgggctgg atcatcgcag
     8941 gatcagtggc gggcatcgtc gccgagatct ggaattatca cgctgtgttc tggtttgcga
     9001 tggtgatgat tatcgccact ctgttttgct tactgcggat taaagatgtt taaggcgcgg
     9061 tcagcatttc cagatcgata agccaggtca tggcctgctg acgcgagtta ccgcacattt
     9121 cagcgctggc ttgtaagcct gcacagactt tcggtcgcag tggcgaggtg aaaattttac
     9181 agcgttgctg ttcatcaagc tgaatgcagg gcgtattggc gggcttgcca tctggcatac
     9241 cgggaatagg gctggaaatt gaaggggcag tgcaacacgc cccgcaaccc ggacggcatt
     9301 ccatacgttg ttctcttaag gttaaccacc ggattgatga gcgcgcacag taccaccttt
     9361 tttgcaccag caaaagtgcg aataccactt gcccgaaagg cccgtcgcga gtactttgtc
     9421 gcgatatttt tgacattttc gactacagga atttttcgat gccaagagcg aacgaaatta
     9481 aaaaaggtat ggtactgaat tacaacggca aactgctgct ggttaaggat attgatattc
     9541 agtcgcccac tgcccgcggt gccgctacgc tgtacaaaat gcgtttttct gatgtccgta
     9601 ccgggctgaa agtagaagag cgtttcaaag gtgatgatat cgttgacacc gtgacgctga
     9661 cccgccgtta cgttgacttc tcctatgtcg atggcaacga atatgtcttt atggataaag
     9721 aagactatac cccgtatacc ttcaccaaag atcagattga agaagagttg ctgtttatgc
     9781 cagagggcgg catgccggac atgcaggtgc tgacctggga cggtcaactg ctggcgcttg
     9841 agctgccgca gaccgttgat ctggaaatcg ttgaaaccgc accaggcatc aaaggggcat
     9901 ctgccagcgc ccgtaacaaa ccggcgacat tgagcactgg tctggtgatt caggtaccag
     9961 aatacttaag cccgggcgaa aaaattcgta tccatatcga agaacgccgt tatatggggc
    10021 gtgctgacta acttcagccg catgcagaaa agggatagct caggctgtcc cttttttaat
    10081 ttattatacc aatcttctat tttgcgcttc ctgaacacct tacgccctca attttcactc
    10141 gttgattgat cgccctcaca cttcatcgca ttaacaatcc agaccaattt caattgctgt
    10201 catataactt tacactgtca ttgttaattt atcgttacta agacgtgact cctatgaaga
    10261 caattgcctc cgttacgctc ccgcatcatg tacacgctcc acgctacgat cgccagcagt
    10321 tgcaatcacg tatcgttcat tttggctttg gcgcctttca ccgcgctcat caggcgttac
    10381 tgaccgatcg tgtgctgaat gcccagggcg gcgactgggg gatctgtgaa atcagcttgt
    10441 tcagcggtga tcaactgatg agccagctcc gcgcacagaa ccatttatat accgtgctgg
    10501 agaaaggtgc ggacggcaat caggtgataa ttgtcggtgc cgttcacgaa tgccttaatg
    10561 caaaactgga ttccttagcg gcaattattg agaaattttg cgagccacag gtggcaattg
    10621 tttccctgac gattaccgaa aaaggctatt gtattgaccc ggccaccggt gcactcgaca
    10681 ccagtaatcc gcggattatt cacgatctac aaacccctga agaacctcac tccgcaccgg
    10741 gtattctcgt cgaagcactg aaacgccgcc gtgagcgcgg ccttacaccg tttaccgtgc
    10801 tctcctgcga caatattccc gacaatggcc atgtggtgaa aaacgcggtg ctgggaatgg
    10861 cagaaaaacg ttcgccagaa ctcgccgggt ggataaaaga acacgtcagt tttccgggaa
    10921 ccatggtcga ccgcattgtt ccggctgcaa ccgacgaatc actggcagaa atcagccagc
    10981 atctgggggt aaatgatccc tgcgcgatta gctgcgaacc gtttatccag tgggtggtgg
    11041 aagataactt cgtcgctggg cgtcctgcct gggaagtcgc aggtgtacaa atggtgaatg
    11101 atgtcctgcc atgggaagag atgaaactgc gtatgcttaa tggcagccac tcttttctcg
    11161 cttatctggg ttacctttca ggattcgccc atatcagtga ttgcatgcag gatcgcgcat
    11221 ttcgccatgc cgccagaaca ttaatgctga atgagcaagc gccgacacta caaattaaag
    11281 atgtcgattt aacacaatat gcggacaagt taattgcacg ttttgctaat ccggcgctga
    11341 aacataagac ctggcaaatc gcaatggatg gtagccagaa attaccgcaa cgtatgctgg
    11401 caggtattcg catacatctg gggcgcgaaa cggactggtc gttgctggca ttaggcgttg
    11461 ctggctggat gcgttacgtc agcggcgttg atgatgccgg aaatgccatt gatgttcgcg
    11521 atccgcttag cgataaaatt cgcgaacttg ttgcgggcag cagcagtgaa caacgcgtaa
    11581 ccgccctgct ttccctgcgt gaagttttcg gtgatgatct gccagataac ccgcattttg
    11641 tgcaggccat cgaacaagcc tggcaacaaa tcgtacaatt cggcgcacat caggcgctat
    11701 taaacaccct caaaatttaa cgatttctgc ggttaaagcg gatgaagctc accttcgtcc
    11761 gctctcccct tctcttttct gcctttttta gccaggatta acgctcagtt aacttaccag
    11821 agtattgcgg agccgttgtg accaggacca acctcatcac cggttttctc ggcagcggga
    11881 aaaccacgtc gattcttcat ctgttagccc ataaagatcc caacgaaaaa tgggcggtac
    11941 tggttaatga atttggggaa gtcggaattg atggtgcttt gctcgccgat agcggcgcat
    12001 tgctgaaaga gatccccggc ggctgcatgt gctgcgttaa tggtttaccc atgcaggtag
    12061 ggttgaatac cttactgcgt cagggaaaac cagaccgctt gttgatagag ccgaccgggc
    12121 tgggccatcc gaaacagatc ctcgatctgt taaccgcacc agtctatgaa ccgtggatag
    12181 atctgcgcgc caccttgtgc attctcgatc cacgcctgct gctggacgaa aaaagcgcca
    12241 gcaatgaaaa cttccgtgac cagctggctg ccgcagacat cattgtcgcc aataaatccg
    12301 accgtgcgac gcccgaaagt gagcaagcgc tacagcgttg gtggcagcaa aatggtggcg
    12361 atcgacaatt aattcacagt gagcatggga aagttgacgg tcatcttctg gatttgccgc
    12421 gtcgcaattt agccgagttg cccgccagcg ccgcgcattc tcatcagcat gtcgtgaaaa
    12481 aagggttagc agcgttaagc ctgccagagc atcaacgctg gcgtcgcagt ctgaacagcg
    12541 ggcaaggata tcaggcctgc ggctggatat tcgacgctga tacggtattc gacaccattg
    12601 gcattctgga atgggcgcga cttgcaccgg tggaacgcgt caaaggcgtg ctgcgtattc
    12661 ccgaagggct ggtacgaatc aaccgtcagg gcgatgacct gcacattgaa acgcaaaacg
    12721 ttgcgccacc ggacagccgt attgagctga tttccagcag cgaagctgac tggaatgctt
    12781 tgcagagcgc gctgttgaag cttcgtttag cgactaccgc gtaaggttgc ctgcgttttt
    12841 cagtaagata attagagaaa atatgattaa aaatttgccg caaatagtgt tgttgaatat
    12901 tgtcggcctc gcgctgtttc tttcctggta tattcccgtt aatcatggat tctggttgcc
    12961 aattgatgcg gatatttttt atttctttaa tcagaaactg gtcgaaagta aggccttttt
    13021 gtggctggtt gcattgacca acaatcgcgc tttcgacggt tgttctctgc tggcgatggg
    13081 aatgttgatg ctgagtttct ggctgaaaga aaacgcccct ggcagacgac gtatcgtgat
    13141 tatgggtctg gtcatgctat taactgcagt ggtattaaac cagctgggtc aggcattaat
    13201 tcctgtaaaa cgggccagcc caacattgac ttttaccgat attaaccgcg tcagcgaact
    13261 gctctctgtt cccacgaaag atgcctcacg agatagcttt cccggcgatc acggcatgat
    13321 gctgcttatt ttttcggcat tcatgtggcg ttatttcggc aaagttgcag gccttatcgc
    13381 ccttattatt tttgtggttt ttgcatttcc cagagtaatg attggcgcac actggtttac
    13441 tgacatcatt gtcggttcga tgaccgtgat attgatcggt ttgccctggg tgttgctgac
    13501 gccattaagt gatcgattaa tcaccttttt tgacaaatca ctaccgggaa aaaacaaaca
    13561 tttccaaaac aaataactca cagtaattaa catcatcagg cttattttta tagtgaggat
    13621 aatcctgatg atgcgcaccg tgctttcatc catcgcgcgc aaaaatcatt ctctaagtaa
    13681 atgaatggat tgcatgcgtt tcactcaatc gtactttaat tgaccaatcc cgcttattaa
    13741 ctttctgtat cactttttct tataaaaaat catgtaaaac cgctcgccaa gaccgcacca
    13801 atcgggtaat ctcgaactcg ttttgcctcg gcggtagatt atcctcacag catataattt
    13861 tgtgcgttag tccacagatt tggccttaag gaattgtttc aacatgccca ggtaattagt
    13921 ctcgtgtcgc ttggcatttt tttataacga tatttgtcgt taaggacttc aagggaaaac
    13981 aaacaacatg gtcaaatctc aaccgatttt gagatatatc ttgcgcggga ttcccgcgat
    14041 tgcagtagcg gttctgcttt ctgcatgtag tgcaaataac accgcaaaga atatgcatcc
    14101 tgagacacgt gcagtgggta gtgaaacatc atcactgcaa gcttctcagg atgaatttga
    14161 aaacctggtt cgtaatgtcg acgtaaaatc gcgaattatg gatcagtatg ctgactggaa
    14221 aggcgtacgt tatcgtctgg gcggcagcac taaaaaaggt atcgattgtt ctggtttcgt
    14281 acagcgtaca ttccgtgagc aatttggctt agaacttccg cgttcgactt acgaacagca
    14341 ggaaatgggt aaatctgttt cccgcagtaa tttgcgtacg ggtgatttag ttctgttccg
    14401 tgccggttca acgggacgcc atgtcggtat ttatatcggc aacaatcagt ttgtccatgc
    14461 ttccaccagc agtggtgtta ttatttccag catgaatgaa ccgtactgga agaagcgtta
    14521 caacgaagca cgccgggttc tcagccgcag ctaataaacc gtttggatgc aatcccttgg
    14581 ctatcctgac gagttaactg aaagcactgc ttaggcagtg cttttttgtt ttcattcatc
    14641 agagaaaatg atgtttccgc gtcttgatcc aggctatagt ccggtcattg ttatctttta
    14701 aatgttgtcg taatttcagg aaattaacgg aatcatgttc atacgcgctc ccaattctgg
    14761 acgtaagctc ctgcttacct gcattgttgc aggcgtgatg attgcgatac tggtgagttg
    14821 ccttcaattt ttagtggcct ggcataggca cgaagtcaaa tacgacacac tgattaccga
    14881 cgtacaaaag tatctcgata cctattttgc cgatctgaaa tccactactg accggctcca
    14941 gccgctgacc ttagatacct gccagcaagc taaccccgaa ctgaccgccc gcgcggcgtt
    15001 tagcatgaat gtccgtacgt ttgtgctggt gaaagataaa aaaacattct gttcatctgc
    15061 gaccggtgag atggacattc cactcaatga attgattccg gcgctcgaca ttaataaaaa
    15121 cgtcgatatg gcgatcttac ccggcacgcc gatggtgccg aacaaacccg caatcgtcat
    15181 ctggtatcgc aaccctttgc tgaaaaatag cggcgtcttt gccgctctga atctcaacct
    15241 gacgccttct ctcttttata gttcacggca ggaagattac gatggcgtcg ccctcattat
    15301 tggtaatact gcgctatcta ccttttcttc acgtttgatg aacgttaacg aattaaccga
    15361 catgccagtc cgtgaaacta aaattgcggg cattcctctg accgttcggc tttatgcaga
    15421 tgactggaca tggaacgatg tgtggtacgc atttttactg ggcggcatga gtggaactgt
    15481 cgttggcctg ctctgctatt acctgatgag cgtacgtatg cgccccggca gagaaatcat
    15541 gaccgccatc aagcgcgaac aattttacgt tgcgtatcaa ccggtggtgg atacacaagc
    15601 tttgcgagta acgggcctgg aagtactgct acgctggcgg catcctgtag cgggagaaat
    15661 tcccccggat gccttcatta actttgccga atcgcaaaag atgattgtgc cgctgactca
    15721 gcacctgttt gagttgattg cccgcgatgc cgcagaatta gaaaaagtac tgccggtagg
    15781 cgtcaaattt ggcattaaca ttgcgccgga ccatctgcat agcgaaagct ttaaagctga
    15841 tatccagaaa ctgctcacgt ccttgcccgc acaccatttc cagattgtgc tggaaattac
    15901 cgagcgcgat atgctgaaag agcaagaagc cacacaactc ttcgcctggc tgcactcggt
    15961 cggcgtagaa attgctattg atgacttcgg caccgggcac agcgcgctta tctatcttga
    16021 gcgttttacg ctcgattatc tgaaaattga ccgtggattt atcaacgcca tcggtacgga
    16081 aacgatcact tcccccgtac ttgacgcggt gctgacgctg gcgaaacgcc tcaatatgct
    16141 gacggttgct gaaggggtcg aaacgccgga acaggcgcga tggctaagcg aacgcggcgt
    16201 taatttcatg caaggctact ggattagtcg cccgttaccg ctggacgatt ttgttcgctg
    16261 gctaaagaaa ccgtatacgc cgcagtggta aggtgtgctt acgtccctta ttattcatag
    16321 tgaaagcatg ccggattgcg gctaatgatg agtaaaagga aatccgttgc agatgattgt
    16381 gcgcatactg ctgctgttta tcgctctgtt cacctttggt gtgcaggcgc aggctatcaa
    16441 ggaaagctat gcctttgccg tgctgggcga accccggtac gcgtttaatt tcaaccattt
    16501 tgattatgtg aaccccgccg cgccaaaagg tgggcagata acgttgtcag ccctcggcac
    16561 cttcgataat ttcaaccgct atgcactgcg cggcaacccg ggcgcacgca ccgagcagct
    16621 gtacgacacg ctatttacga cttccgatga cgaaccaggc agttattacc cgctgattgc
    16681 tgaaagcgca cgctatgctg acgattattc ctgggtggag gtcgctatta atccgcgcgc
    16741 ccgttttcat gatggttcgc ccattactgc ccgcgatgta gagtttactt ttcaaaaatt
    16801 tatgaccgaa ggcgtgccgc aatttcgtct ggtctacaaa ggcaccaccg tcaaagccat
    16861 tgcgccgtta accgtgcgca ttgagttagc taaacccggc aaagaagata tgttgagtct
    16921 gttttcgctg ccggtatttc cagaaaagta ctggaaggat cacaaactta gcgacccgct
    16981 cgccacgcct ccgcttgcca gtggtccgta ccgcattacg tcctggaaaa tggggcaaaa
    17041 tattgtctat tcccgcgtga aagattactg ggcagcaaac ttaccggtaa accgcggacg
    17101 ctggaatttc gacaccattc gctacgatta ttacctcgat gataatgtcg cctttgaagc
    17161 gtttaaagca ggtgcctttg atttgcgtat ggaaaacgac gccaaaaact gggccacgcg
    17221 ttataccggt aaaaatttcg ataaaaaata catcatcaaa gatgagcaaa agaacgaatc
    17281 agcccaggat acgcgctggc tggcgtttaa tatccaacgt ccagtattca gcgatcgccg
    17341 ggtgcgggaa gcaatcactc tcgcctttga ctttgaatgg atgaacaagg cgttgtttta
    17401 caatgcctgg agtcgcacga acagttattt tcagaatacc gaatacgcgg ccagaaatta
    17461 ccccgacgcc gcggagctgg tgcttctggc accaatgaaa aaagatctac cgccagaagt
    17521 cttcacacaa atctaccagc cgccggtatc caaaggcgat ggctacgatc gtgacaacct
    17581 gttaaaagcc gacaaacttc tcaacgaagc gggctgggtg ctgaagggtc agcaacgcgt
    17641 taatgccaca acgggtcagc cactcagctt tgaattattg cttcccgcaa gcagcaatag
    17701 tcagtgggta ttgccgttcc agcacagcct gcaacggctg ggtatcaaca tggacattcg
    17761 caaggtggat aactcgcaaa tcactaaccg catgcgcagt cgcgactatg acatgatgcc
    17821 gcgcgtatgg cgggcgatgc cgtggcccag ttccgattta cagatttcct ggtcatcgga
    17881 atatatcaat tccacttata atgcccccgg cgtgcaaagc ccggttatcg actcgctgat
    17941 caaccaaatt attgccgcgc agggaaataa agaaaaatta ctgccgttgg ggcgagcact
    18001 ggatcgcgta ttaacgtgga attattacat gctgccaatg tggtacatgg cggaagaccg
    18061 tctcgcctgg tgggataaat tctcccagcc ggccgtgcgc cccatctata gcctcggtat
    18121 cgatacctgg tggtatgacg tcaataaagc ggccaaactg ccgtccgcca gcaaacaggg
    18181 agagtagatg ggcgcttacc tgattcgccg tctgttgctg gtgatcccaa cattatgggc
    18241 gattatcacc atcaactttt tcatcgtgca aattgcgcct ggcggtccgg tcgaccaggc
    18301 catcgccgcc attgagtttg gtaatgccgg agtattaccc ggcgcaggcg gtgaaggtgt
    18361 tcgtgccagc catgcgcaaa cgggtgtcgg caatatcagc gacagtaatt accgtggcgg
    18421 acgcggatta gatccagaag tgatcgctga gatcactcat cgctacggtt ttgataagcc
    18481 gatccacgaa cgttacttca aaatgctctg ggactacatc cgctttgatt ttggcgatag
    18541 cctgtttcgc agcgcctcgg tgctgacgct gattaaagac agtctgccgg tttccatcac
    18601 cctcggattg tggagcacgc tgattatcta tctggtgtcg attccgttag gcattcgcaa
    18661 agctgtttat aatgggagcc gctttgacgt ctggagtagc gcatttatca tcatcggcta
    18721 cgccattccg gcctttttgt ttgccatcct gctgattgtc ttcttcgcgg gcggcagcta
    18781 tttcgacctg ttccctctac gcggcctggt ttccgctaac tttgattcgc tgccgtggta
    18841 tcagaaaatc accgattatc tgtggcatat cacgctgccg gtgctggcga cagtgattgg
    18901 tggctttgcg gcgctgacca tgctgacaaa aaactcattc cttgatgaag tgcgcaagca
    18961 atacgtggtg accgcgcgtg cgaaaggggt aagtgaaaaa aatattctct ggaaacatgt
    19021 gttccgcaac gccatgctgc tggtgattgc cggttttccg gcgacgttta tcagcatgtt
    19081 ttttaccggc tcgctgctga ttgaggtgat gttttcactc aatggtctgg gcttactggg
    19141 ctacgaagcg accgtctcgc gcgattatcc tgtaatgttt ggtaccttgt atattttcac
    19201 cctgattggc ctgctgctga atattgtcag tgatatcagc tatacgctgg ttgatccgcg
    19261 tattgatttt gagggacgtt aatgtcgcga ctcagccccg tcaatcaggc ccgatgggcg
    19321 cgttttcgtc ataaccgtcg cggctactgg tcgttatgga ttttcctcgt cttgtttggt
    19381 ttgagtttgt gttctgaact tatcgccaac gataaaccgt tgctggtgcg ttatgacggc
    19441 agttggtatt tcccgttatt gaaaaactac agcgaaagcg attttggcgg cccgctggca
    19501 agtcaggctg attatcagga cccgtggctg aaacaacggc tggaaaataa cggctgggta
    19561 ctgtgggcac cgattcgctt tggtgctacc agtatcaact ttgctaccga taagcccttc
    19621 ccttctccac cctcccggca aaactggctg ggaacggatg ccaacggcgg cgatgtgctg
    19681 gcacgcattc tctatggcac gcggatctcg gttctgtttg gcctgatgct gactctctgt
    19741 tccagcgtga tgggcgtgct ggcgggggcg ctacaaggct attacggcgg taaagtcgat
    19801 ctctggggac aacgctttat tgaagtatgg tcggggatgc cgacgctgtt tttgattatt
    19861 ttactttcca gcgtcgtaca gcctaacttc tggtggctgc tggcaattac tgtcttgttt
    19921 ggctggatga gtctggtcgg cgtgatgcgg gcggagtttt tacgtactcg taatttcgac
    19981 tacattcgtg cggcacaggc gcttggcgtc agcgatcgca gtatcatcct gcgtcatatg
    20041 ttgcctaatg ccatggtcgc gaccctcacc tttttaccgt ttattttatg tagttcgata
    20101 accaccctga cctcgctcga tttcctcggc ttcggtctac cgctcggttc accgtcactc
    20161 ggtgaactgc tgttacaagg gaaaaataac cttcaggccc cgtggcttgg gatcaccgcc
    20221 ttcttgtcgg tggcgatatt attgtctttg ctgatcttta ttggtgaagc cgtccgcgac
    20281 gcatttgatc ctaataaggc ggtgtagcat gacgcaaact ctgttagcga ttgaaaattt
    20341 gtcggtgggt tttcgccatc agcaaaccgt acgtacagta gtcaatgatg tttcactaca
    20401 gattgaggct ggcgaaacgc tggcgctggt gggtgagtca ggttcaggca aaagcgttac
    20461 cgcgctgtca attttacgcc tgctcccttc cccgccggtt gaatatctct ccggcgatat
    20521 tcgttttcat ggcgaatcgc tgcttcatgc cagcgatcaa acgttgcgcg gtgtacgcgg
    20581 taataagatc gccatgattt ttcaggaacc gatggtgtcg ttaaatccat tgcataccct
    20641 ggaaaaacag ctttatgaag tgctttcact ccaccgcggg atgcgtcggg aagcggctcg
    20701 tggcgaaatt cttaactgcc ttgatcgcgt tggtatccgc caggcggcaa aacggctgac
    20761 agattatccg catcagctct ccggcggcga acgccagcgg gtgatgattg cgatggcgct
    20821 gttaacgcga ccggaattat taattgccga tgaaccgacc accgcactgg acgtctctgt
    20881 ccaggcgcag attttacagc tgttgcgcga actgcaaggc gagctgaata tgggcatgct
    20941 gtttattact cataacctca gcattgtcag aaaactggcc caccgcgtgg cggtaatgca
    21001 aaacggtcgc tgtgtcgagc aaaataacgc cgctacgcta tttgcctcac ccactcatcc
    21061 ttacacacaa aagctactca acagtgaacc gtcaggcgac ccggtgccgt tgccagaacc
    21121 tgcctcaacg ttgctggatg ttgaacagct tcaggttgcc ttccccattc gcaaagggat
    21181 tttgaagcgc attgtggatc ataatgtggt ggtgaaaaac atcagtttta cgctacgagc
    21241 gggtgaaaca ctgggtttag tgggcgagtc cggttccggg aaaagtacga cgggactggc
    21301 gctgctgcga ctgattaatt ctcagggcag catcatcttt gacggtcagc cattgcaaaa
    21361 tttaaatcgc cgccagctgt tacctattcg tcatcgcatt caggtggtat ttcaggatcc
    21421 aaactcctcg ctcaacccac gactcaacgt tttgcagatt attgaggaag gcttacgggt
    21481 tcaccagccg acgctttctg ccgcacaacg cgaacaacaa gtgatagccg tgatgcatga
    21541 agtgggatta gatcctgaaa cacgccaccg ttatccggcg gagttctctg gtggtcagcg
    21601 acaacgtatt gcgattgcca gggcattaat tcttaagccc tcgctgatca tacttgatga
    21661 accgacatca tcactcgaca aaacggtaca ggcgcaaata ttgacgctat tgaaatcatt
    21721 gcaacaaaag catcaactgg cctatttgtt tatcagccac gatttgcacg ttgtccgcgc
    21781 gttatgtcat caggttatcg tactgcgaca aggggaagta gtggaacaag gaccgtgcgc
    21841 gcgcgtgttt gccgcaccgc agcaggagta tacgcgtcag ctactggcgt tgagctgacg
    21901 cttaaaaagg attgtagttt gaaaagggtt cggcaattgc cacaccaaaa tttttcaatc
    21961 ggcatgttgc agcaaactcg tcctggcgat ttacaaacag gcacggctca ccttcacact
    22021 ccagcaccga acatggaact tcgatgtcgc ttaacgcctg gcttagtttg tacatcaccg
    22081 accatgcatt atcaccatcc gggctaagca atttaagcgc caccaggctg ttgtcaccgc
    22141 acggtccatt ttgcggaatc ggttcaacct tcgaacctgc gaaaccagca gaccagcgaa
    22201 agttctgcgg tagacgatga acgattgaga gttgtaatga agtcacttat tttccccgga
    22261 agtacattta cttcacaatt tgtttacatc aattttaaca catcatcaac aatccgtctt
    22321 tatacagatc gtaaagattg atgcctcgcc tgcgtggcct ctatggctct gatttaagta
    22381 atatctgcgg gcgtggtcac gtttgcgggg ctatatgtac ccgtttctgc gatctaactc
    22441 aaccttttta actacaatga tgtgactttt tacataaatt gattttacat aaaataaaca
    22501 tatatcgggg gaatgataga tttgtggttg acatgcatca acaaaggaag ccttttagct
    22561 tcctcgttgt gcaatagatc accgtttttt cggtcgactg gcgtacagac agaagagaat
    22621 ggagctggtt gcgcagaatg caattgacca aatcatcggc catgcagagt taaaggtcgc
    22681 aagagaaagc aatgcgccaa caattgcccc tatgccaaaa cggaaggttc ctgccagcga
    22741 agatgccgtt cccgccatat ggggaaactc atcaagaatg accgccatcg cattggatga
    22801 caccatcgac acgcagccca caaacgccgc aacgccaacc accagcgacc agaatcccag
    22861 ccccagcagc gcactgatga ccatccacgc cgccataata aactgtatcc acaaccccga
    22921 gcggaacata tttaacgcgc caatacggcg gacgaagcgg ctgttaaaaa tggtcatcac
    22981 gaacaggaaa acaatgttta gtgcaaagta ataaccaaag ttttccggcg cgacgtggtt
    23041 aatttcaatg tagacaaacg gtccggcgct caggaacgag aacattccgg caaagctgaa
    23101 accgctggcc agcatataac tcaggacacg cttatgacgg aacagcgccg caaagttacc
    23161 aatagtggta cgaatgtgaa aaggctggcg acgctccggt ggtaaggttt ctttgatcag
    23221 aaagaagatc attgccgaag ccagaatcgc cgccacggcg aggatccaga agatgtaatg
    23281 ccagctcagc cacaccagca cccagccgcc aactatcggt gccatcagcg gtgcaatggt
    23341 tgtcaccagc atgacaaacg acatcatccg cgagaactct tctttcgggt agatatcacg
    23401 catcaaagcg ttgataacca cgctggccgc agccgccgcc agcccgtgga agaaacgcat
    23461 cacaatcagc tgatcgatgg tttgtgccaa cgcacacgcc accgcggcgg cggcaaacac
    23521 cagcgtaccg ccaagaacca ccggcttacg cccgaagctg tctgccatcg gcccgtagat
    23581 taactgcccc aacgcaaagc ccagaatata agtactgagg gtcatctgcg tactgcccgc
    23641 cggtacgcca aactgcgctg aaattaccgg tagcgcgggc agatacatat caatcgacag
    23701 cggcatcaac atggccagca ggccaaggat aaaaacaata gcaaacgacg aatgctgtcg
    23761 ggtggtcaca acgggctcct gaaagtcatt gaaaagttag acgacgctgg caatttcttc
    23821 ttcagttaac ggacgatatt caccgggggc taaatcagca tccagcgtaa tgccgccaat
    23881 acgttcacga tgcagctcaa ccacatggtt acccacggcg gcgaacatgc gtttcacctg
    23941 atgataacgc ccttcgctga tggtcagacg aacctgcgtt ggggtaatca cttccagcac
    24001 cgccggctta gtgagatctt tttcgttatg cagctgcacg cctttagcaa attgctctgc
    24061 cgtatcgtca gctacgggtg attccagcgt caccagatag gtcttctcgc aatgatggcg
    24121 cggagaagta atgcggtgcg accactgacc atcatctgtc atcagcacca gaccggtggt
    24181 atcgatatcc agacgccctg cggcgtgcag tttccaggct accggttcat caagaaaata
    24241 gagcaccgtt gggtgatcag ggtcgtccgt ggagcaaacg tagccctgag gcttattgag
    24301 catgaagtaa cgtggaccgt gttgctgcgc cagcgggttg ccatcgtaag cgacatcatg
    24361 ttcaggaagc agcttgaacg ctgcattacg gacgatttcg ccatcgacgg tgacacgatt
    24421 gccgcggatt tcacgcccgg caatagcacg gctaacgccg agttgctgtg cgataaattt
    24481 atcaagtcgc atgtgtgtga ttttgcctgt aaaaaacgga ggtcgggtaa tgacccgaaa
    24541 atctgaactg ttgtctgccc agtatagcgg tctaataaca tccctcaagg gaaaaagatc
    24601 catggcatac tattagcaga ataatctacc tacgcgagac catgattttt acacttcgcc
    24661 catatcagca agaagccgtg gatgccacgc tcaaccattt tcgtcgtcat aaaacccctg
    24721 ccgttatcgt gctgcctact ggtgcaggta aaagcctggt gatagcggaa ctggcgcggc
    24781 tggcgcgtgg tcgcgtgctg gtgctggcac acgttaaaga actggtggcg caaaaccatg
    24841 caaaatatca ggcactggga ctggaagccg atatttttgc cgccgggcta aagcgcaaag
    24901 agagccacgg taaagtggta tttggcagcg tgcagtcggt cgcccgtaat cttgatgcct
    24961 ttcagggtga attttcgctg ttgattgtcg atgaatgtca ccgtattggt gatgatgaag
    25021 agagccagta tcagcaaatc ctcactcacc tgacaaaagt gaatccccac ttacgcctgc
    25081 tggggctgac tgccacgcct tttcgattgg gcaaaggctg gatctaccag ttccattatc
    25141 acggcatggt acgcggcgat gagaaagccc ttttccgtga ctgcatttat gagctgccgc
    25201 tgcgttatat gattaaacac ggctatctga cgccgccaga acgactggat atgccagtag
    25261 tacaatacga tttcagccgc ttgcaggcac agagtaacgg gctgttcagc gaagctgatc
    25321 tcaaccgtga gctgaaaaaa cagcaacgca ttacgccaca tatcatcagc cagatcatgg
    25381 agttcgccga aaagcgcaaa ggggtaatga ttttcgccgc gacggtcgaa cacgcaaaag
    25441 agattgtggg attactgccc gccgaagatg ctgcactgat tactggcgac acccccggcg
    25501 ctgagcgcga tgtgttaatt gaagatttta aagcccagcg ttttcgctat ctggtcaacg
    25561 tcgcagtact gaccaccgga tttgacgccc cgcacgtcga tcttatcgcc attctgcgcc
    25621 ctaccgaatc agtgagtctt taccaacaaa ttgtcgggcg cggtctgcgt ctcgctcctg
    25681 gcaagactga ttgcttaatt cttgattatg cgggtaatcc tcacgatctc tacgcgccgg
    25741 aagttggtac accgaaaggc aaaagtgaca acgtcccggt acaggttttc tgccctgcct
    25801 gcggttttgc caacaccttt tgggggaaaa cgaccgccga cgggacattg attgaacact
    25861 ttggtcgtcg ctgtcaggga tggtttgaag atgacgacgg tcatcgcgaa cagtgtgact
    25921 tccgtttccg ttttaaaaat tgcccgcaat gtaacgcaga aaatgatatt gccgcccgcc
    25981 gctgccgcga atgtgacacc gtactggttg atccggacga tatgttaaaa gcggcgctac
    26041 gactgaaaga cgcgctggta ttacgctgta gcggcatgtc tttgcaacat gggcacgacg
    26101 agaaaggcga atggttgaaa atcacctatt acgatgaaga cggcgcggat gtgagtgagc
    26161 gtttccgtct gcaaacgccc gcccagcgaa ctgccttcga gcagcttttt atccgcccgc
    26221 atacgcgcac accgggcatc cctctgcgct ggatcaccgc cgccgatatc ctcgcccagc
    26281 aagcgttact gcgacacccg gattttgtcg tcgcccgcat gaaaggtcag tactggcaag
    26341 tgcgtgaaaa agtgttcgat tacgaaggtc gttttcgtcg ggcgcacgaa ttacgcggtt
    26401 aatggtagtt ttgattgatg tataaggcga ttgagtatag aatctcgccc gcttttgcat
    26461 acgcaaagca gatcacttac ctgttgctgg gtcgcctgta gcaggattaa tttaagagag
    26521 aaagaaatgt ttactatcaa cgcagaagta cgtaaagagc agggtaaggg tgcgagccgc
    26581 cgcctgcgtg ccgctaacaa gttcccggca atcatctacg gtggcaaaga agcgccgctg
    26641 gctatcgagc tggatcacga caaagtcatg aacatgcaag ctaaagctga attctacagc
    26701 gaagttctga ccatcgttgt tgacggtaaa gaaatcaaag ttaaagctca ggacgtacag
    26761 cgtcacccgt acaaaccgaa gctgcagcac atcgacttcg ttcgcgctta attgctgaat
    26821 aagttgtaaa aaaccccgct ccggcggggt tttttgtatc tgcagattat gcctgattac
    26881 ggtattgcta ttttttgcag gccagataag gcgttcacgc cgcatctggc attcagaaca
    26941 caaaaccgat taattgccgc cagacgtccg gcgctgtaat tggtcgcgca aattcggcgg
    27001 cgtgccttta atggtcaagg tatcggttgc tggatcccag aagatgcgtt cacctaacag
    27061 catcgcatca aagttaatag tcaagccacc gccgctaccg gcaaacttcg tcaactgacg
    27121 cagtgtgcta cggtctgccg ggaagctctc ttccagttcg tagccttttt cagccgcaaa
    27181 ctccgtgaaa ctcacttcgc tgacgcctgc cagctctttc gacagcgact ccagttcaat
    27241 ctcttcgcct gcttgcagct gctcgttaca gtagctataa acctgctggc gcacattctg
    27301 ccgctcggct ttatccagtt gcgcttgtgc agtgaaatca tccaccgctt gcagcaagcc
    27361 acggttttgc gcctttgcat tcagcccttc tctggcacca aggaaatcca taaagaaatc
    27421 ggcgaccttg cgccctaccc gccctttcag gaaagttaaa tagcgggtcg attccgggtt
    27481 ggtttcccat tcggttaaat cgatgcgcgc gacgatatcc gcatggttga tatcgagata
    27541 gtgcgttggg ttgatgtcga gattttcgtt aacgcgcata ctgctcaggt tgctcaggac
    27601 tgccaccagc agatactcca ccgccagata acggtagtgg caaaacagca cgaatccacc
    27661 gtcagcaaaa ggatatttcg ctaattcgtc acgcaggcga ccagttgccg cgcggctaaa
    27721 tgccagaaag tcctcttcgc cctgacgctg taagcgcagc gtctgcgcca gttcactctc
    27781 ttcgctaaac aagccgtaag ctttattttt ggcgctatag acccgatgca gttctgccac
    27841 catctcaacg acggtttctg tcggttccag caatgaatcg cgcaacacca gctcaaggtt
    27901 ctgctcatca cgcttgataa gctggtgcag agcaatctgg ttgatatcca gactcatgat
    27961 aaactctcct ttaagaccgg gcggtattca accaccgccg gttaagcgac gcaataaaag
    28021 atggcggtca ttttacagaa ggcagcgtaa cgagaatgat ttaacgcata aaaaagcaga
    28081 aaaaaaaccg ttgctacggt aatatgttgc cctttcatca actaactgat ttcgatttat
    28141 gccacaaatt tcccgctaca gtgatgaaca ggttgaacaa ttgctcgcag agctgctcaa
    28201 cgtactggaa aaacataagg ctccgaccga cctttccctg atggtgttag gcaacatggt
    28261 cactaacctt atcaacacca gcattgcccc ggcccaacgc caggcaattg ccaactcttt
    28321 tgcccgcgcc ttacagtcct ctatcaacga agacaaagcg cactaaggga aacagataac
    28381 aggttatggt aactcatcgt cagcgctacc gtgaaaaagt ctcccagatg gtcagttggg
    28441 ggcactggtt tgcactgttc aatattctgc tttcgctcgt cattggcagc cgttacctgt
    28501 ttatcgccga ctggccgacc acgcttgctg gtcgcattta ttcctacgta agcattatcg
    28561 gccatttcag cttcctggtg ttcgccacct acttgctgat cctcttcccg ctgaccttta
    28621 tcgtcggctc ccagaggctg atgaggtttt tgtccgtcat tctggcaacg gcgggaatga
    28681 cgctattact gatcgatagc gaagtcttta ctcgtttcca tctccatctt aatcccatcg
    28741 tctggcagtt ggttatcaac ccagacgaaa atgagatggc gcgcgactgg cagctgatgt
    28801 tcatcagcgt gccggttatt ttattgcttg aactggtgtt tgcgacgtgg agctggcaaa
    28861 agctgcgcag cctgacgcgt cgtcgacgct tcgcgcgccc gctggccgca ttcttattta
    28921 tcgcctttat cgcctcgcat gtggtgtata tctgggccga tgccaacttc tatcgcccga
    28981 tcaccatgca gcgcgctaac ctgccgcttt cgtacccgat gacggcgcga cgttttcttg
    29041 agaagcatgg cctgcttgat gcgcaggagt atcaacgccg tcttattgag caaggtaatc
    29101 cagacgccgt ttccgttcag tatccgttaa gcgaactgcg ctatcgcgat atgggcacag
    29161 ggcagaatgt cttgttgatt actgtcgatg gcctgaacta ctcacgcttc gagaagcaga
    29221 tgcctgcgct ggcaggtttt gctgagcaaa atatttcgtt cacgcgccat atgagctccg
    29281 gcaacactac agacaacggc atctttggcc tgttctatgg catctcgccg agctatatgg
    29341 acggcattct gtcgacccgt acgcctgcgg cattaattac tgcgcttaat cagcaaggct
    29401 atcagctggg gttattctca tcagatggct ttaccagccc gctgtatcgc caggcattgt
    29461 tgtcagattt ctcgatgccg agcgtacgca cccaatccga cgagcagacc gccacgcagt
    29521 ggatcaactg gctgggacgc tacgcacaag aagataaccg ctggttctcg tgggtttctt
    29581 tcaatggtac taacattgac gacagcaatc agcaggcatt tgcacggaaa tatagccggg
    29641 cggcaggcaa tgtcgatgac cagatcaacc gcgtgctcaa tgcactgcgt gattctggca
    29701 aactggacaa tacggtggtg attatcactg ccggtcgggg tattccactg agcgaagagg
    29761 aagaaacctt tgactggtcc cacggtcatc tgcaggtgcc attagtgatt cactggccag
    29821 gcacgccggc gcagcgtatt aatgctctga ctgatcatac cgatctgatg acgacgctga
    29881 tgcaacgcct gctacatgtc agcacacctg ccagcgaata ttcgcaaggt caggatttgt
    29941 tcaaccctca acgccgtcat tactgggtta ccgcagcgga taacgatacg ctggcaatta
    30001 ccaccccgaa aaagacgctg gtgctgaaca ataacggtaa ataccgcact tacaacttac
    30061 gtggtgaaag agtgaaagat gaaaaaccac agttaagttt gttattgcaa gtgctgacag
    30121 acgagaagcg ttttatcgct aactgattaa ttataaatca gttagcgaaa tatcttactt
    30181 gcaatcggtg tggaaaacgg tagtattagc agccacgagt cggcacgtag cgcagcctgg
    30241 tagcgcaccg tcatggggtg tcgggggtcg gaggttcaaa tcctctcgtg ccgaccaaaa
    30301 atcccaaaaa aaaccaacca ttacggttgg tttttttata tctgcaatta attcgataaa
    30361 cagaccgtga cacatcacag cctgtttatt ttctgttatc agaacgtcca gaccacaccc
    30421 gcctgagtat tccacggcga ttctacccct gcgccattgc catagctgac tgacaaatga
    30481 ccgcttaacg tcggggtaaa cgatgacctt atcccagcct gataaacgcc acgggctcca
    30541 gccacatcat tattaaattt gccatcgtca ttaactttca cctggttaga atcggcgtat
    30601 tcctgacgta cggcggcttt cagccagggt tccagcgtcg taccattttg caggtccatg
    30661 tgatagctta ccgccgttcc cgcttcagcg cgtaatatcc gggtatttcc cacatcagcg
    30721 cgcatgccgt ttgataacgt gtagtcctga ccatctgtgg taaagccggt aaaggccaga
    30781 tagggtctaa cactccacaa tccgtcaacc caacggaacc cgctctcaac atgagcaccc
    30841 gcgccgttac tattgtaatc gccaaacgct gttgccccat tactcatctt gccatggatg
    30901 gtgttggcaa aacggtcaac tttcaccacc ccatcaacat aggcaccgtt ctgatgctcc
    30961 caaccggcat aagcccccag ggtatagcta tcgatattac cttttccgcc gcgatcaaaa
    31021 ccaatatcag aatgagagta accaaagaac aagccgcgaa ttgtactgct ttcttcacgg
    31081 gagaaacggc tatcgatacc gagcgtcagg cccgtcaatg tttgctcaaa accagctccc
    31141 gcatcagtgg tcacgttgtt gcgggtgtta attgccgaac tccacatcgc cgtatcgtaa
    31201 ctaacgcctt ttacgctacc aagacgctca cgcacggtgt ccagttctgc atcaaatacc
    31261 agcggttgtg cggccgccat attcagcaca tcagtggttg aaggggtaat ttgcgcgcga
    31321 ttctctgcca aactccagct atggttgcca ttatccagca aggtatattc atacgtaccg
    31381 atatcaacaa cgcctccagc attgcccaac gtaaatgcag catcaccgcc gcccgttgtt
    31441 accagtgtaa ggctatctcc tgctgccggg ctggcaccgg tgtccgtcac gaatattttg
    31501 aaatcacctg ttgcctgacc ggtgacgttg agctgatcgc tctgatgatt agccatatcg
    31561 gtacgcatat aaaaattgcc gtttcccgac agggtattgg tggtcaacgt aatgaaattt
    31621 tccgcacttg ccgtcactga tgaacgagtc actggctcat ccatcataat cacatttgcg
    31681 tcagacatcg caaggttggc taagttgagg agggtgtttt ggttcgtcgt agtgatatcg
    31741 gcaatgatgg tccatacgct attttcgagc gaaacgtccg ctttgctggt atccacggca
    31801 ccatgcgttc taaaagaagc gccctcagaa accactacct tacccaccaa cattgcgggt
    31861 gcataattta tttgcggctt catgacctca agaatgccat cattccctct gactgaaacg
    31921 ttaaccattg tgccagccca gacgttagcg cggccattgt tgataaccat attttcagag
    31981 atggcttttg aggaatacgt gatacttcca ttctgatctg atcgaccgag atcatacacc
    32041 gcatttgcct gcactctcgt cccagtatcc tttcccagcg attgcatagt ggcatgctca
    32101 tcaaggatag tatcaatggc ctgcgtgtcc tccataacaa tgagcccgga accatcatcc
    32161 agttcataat tttttgacac accatctttt atactaaatt gacctttact gtttgtacca
    32221 ctcacttcca gcgcattcgt tgagacaatt aattttccgc ctgctttctg atctaccgca
    32281 atcgctaatc ccccctcttt aacctccagt aatcctccct tttcgatagc ggcaaggacg
    32341 gtaatcccat ctttttcaac gattaagcgc ccgcctttaa tcgtgggatc attcgctgcg
    32401 ccataaacct gtagcacacc attattgagg gtagtctggt ttgccgcgcc acccatttga
    32461 acaatttgat aaccgtcatt taatactgag tattctgcgg tgccattcat ttcaatgtac
    32521 tgatacccgc cgttaatttc agtattacta cttataccaa attctttaat atgctgttct
    32581 ccgccaggat tgattttcgt attattggcg gtcccataaa ccagctggat atctttttcc
    32641 aggacaacac catcaactgt ctcaccatac tcaactgttg atgccaatgc tatgggtgaa
    32701 tataacgctg tagccaccag aacagatatt gcggaacgac aaagagaaac agaaccagat
    32761 tgatgcattg agctttcatc ctatgaaatt aattgcagtt aaaagcattg ggtacagaaa
    32821 atacccatag ctccataccc ggagtcagtt tttaaaaact gtttaaagaa atgcacaagt
    32881 attgtgattg attttttagt tgtttttctt gatgagaagc tgatgcaaaa ttccgtcttt
    32941 ataatgaaaa tgatgccaaa gcgaacgaca aggttgtagt tttcactaca tgtccataca
    33001 taaaatgggg taacattcac gcgcctggta gcgttaccaa cgctacgctc aaacataatg
    33061 attctaataa aacctcagga gactactatg cctgaagcaa cgccttttca ggtgatgatt
    33121 gtggatgatc atccacttat gcgacgcggt gttcgtcagt tactggagct tgatcctggc
    33181 tttgaagtgg tcgccgaagc gggcgacggc gcgagcgcga tcgatctggc gaatagactg
    33241 gatatcgacg tgatcttgct ggatctcaat atgaaaggta tgagtggcct ggataccctc
    33301 aatgccttgc gcagggatgg cgttaccgcg caaattatta tcctgaccgt atccgatgcc
    33361 tccagcgatg tctttgcgct gatagacgca ggcgcagacg gttatctgtt gaaagacagc
    33421 gacccggaag tattgctgga agcgattcgt gccggagcga aaggcagcaa agtctttagc
    33481 gaacgcgtca atcagtactt acgtgaacgt gaaatgtttg gcgcggaaga agatcccttc
    33541 agcgtgctga cggagcgcga gctggatgtt ctgcacgagc tggcacaagg gctgtcaaat
    33601 aaacagattg cctcggtgtt gaatatttcc gagcagacag taaaagtaca tattcgcaat
    33661 ctgctgcgta aactcaatgt ccgctcacgc gtggcggcca ccattctgtt cctgcaacaa
    33721 cgcggggcac aataaaaata gcccgatggc atcaccatcg ggcctctttt tatttactct
    33781 cctgcggcga caaatgttgc atcgcctgcg cgatactacg ttcaatcacc gcacggcgag
    33841 tatcgttggc aggtaagagt ttcaacatca tctcccacgc ggcaacggct tcgccaaatc
    33901 gctgctgctc aaaggcgtta aacgcataca tgcttaacac acgaatattg ctatggtccg
    33961 ttctcaccaa ctggcgcagc agttcgccgc cgagacggtt gtcgttggga tcagatgaac
    34021 gtgtcaacgc ttcagcgtat cccagtgcag catcgctgtt cttcggatcg aggcgatacg
    34081 cagtggcgta tgcatcggtg gcgatactgg cgttacccag cgccatgcca acgcggccca
    34141 acataatcca gccttctata tcacccggat ttttttgcag ttgagtacgc atccccagcg
    34201 caaggcgcga catctcttct tcgttgagcg gatcggcttt cggatccagc gccctgtcca
    34261 gcaacgccgg agcctgtgcc gtggcctgct gccagatttt cacctgctga taattgccag
    34321 tctggtagta gctgacgcca gccacaatta gcgccaccac aatacccggc agataaacaa
    34381 catatccggc acgcttacct tccggcacgc tttgttcagg aaacgcttcc ggcaccacgc
    34441 gtacccgacg ccgcgaacgg gcgtaaatga cccaaccgcc aatgccaata gccactaccg
    34501 gaagcaccca cagcagcacg gtcagcggcg ttaacggcgg atcgtaagtg acgaagttgc
    34561 cgtaacgcgc caccatataa tcgacaatct cttttttact tttaccttcc tgcatcagtt
    34621 catacacttt ctggcgcagg tcggtggcaa tcatcgagtt ggaatcggca atgctgttgt
    34681 tctggcattt cgggcagcgc agttcttcag tgagctgacg gaactgttgt tcctgtgctt
    34741 catctttaaa ctgcaacacg tcgatggtcg ccagcgctga gccggagatc atcagcatca
    34801 gcacgcccaa taaaaacctc attgtgcggc ctccttactg tatttctccc acagcggctt
    34861 gatctcttct tcccagacgc gaggattcag atcgcccgca tggcgatagc gaatgatgcc
    34921 gttgccgtca ataaggaacg tttctggcgc gccatagaca ccgagatcca gccctaacat
    34981 gccgtcgcca tcaaacaggc ttagcgcgta aggattgccc agctctttca gccagctgat
    35041 tgccttctgg cgatcgtctt tatagttcat gccgaccacg cgaatgccct gcgctgaaag
    35101 ctgattcaga tattgatgtt ccgcacggca ggtcggacac caggtcgccc agacgttaag
    35161 cagtactggt ttgccctgag tcagcacatc cgcctgataa aactgccccg gattgtccag
    35221 tgattcgaga cgaaacttcg gcacaggctt gccaatgagc gccgattcca gattggtcgg
    35281 atcatcccct tcggcattac gcgccagctg ccacagcagc gccgcggcaa tcgccaggaa
    35341 gataatcaac ggaattaaca atactttgcg cttcatacgg cctccggcgc agttttttgc
    35401 ggactcacgc gcttacgata gcgaggatca aacagacaca gtagtccgcc caacgccatc
    35461 atcagcccgc ccgcccaaat ccagcgaaca aatggtttgt agtaaagacg cacggcccac
    35521 gcgccgtttt ccagctcttc accgagcgcg gcgtacaggt cacgcgtgat gccgccgtca
    35581 attgccgctt cggtcatcat cgacccggca gtgttgtaat aacgtttttc cgcatacagc
    35641 accgtttccg gtttaccctc tcgcgttacg ccaatagtcg ccacaccgcc gcgccagttc
    35701 gggccagtca cctctttgac atcacggaag gtgaagcgat attcatgaat atcgacgcta
    35761 tcgccggact tcatgcgcac atcacgctca acgctataat tctggctaaa ggcaatgcca
    35821 acaattgtca ctgccagccc caggtgagcc gccaccatcc cccaataact gaaggtggtt
    35881 ttcgtgccgc gtgaaatacg tagcgcagct tccgcaattg ccagcaccgc aatccagcag
    35941 gccattgcca ggccgagcac cgtcatcgcc accactttgc tttcgaacag ccacggcaac
    36001 agcagagaca gcaccagcgt agagatgaag gcgataatca ataaattgcg gattttacgt
    36061 gggcgatcgc gcccccagcg caccagagga ccgacaccca gcagtagcgc aaacggcacc
    36121 atcagccagg taaacatggt gttgaagaac ggttcgccaa tcgaaatact gcccagtccc
    36181 agttgcttgt gcaccagcgg cagcagcgtc cccagcaaca ccaccagcat cgcggcgacc
    36241 agcaaaacgt tgttcgctaa cagcagagat tcccgcgacc acagcgcatt gtttacgcgt
    36301 gagcgaactt tgtgtccacg cgcggcaaac agcagcagcg aaccgccaat caccagcacc
    36361 ataaaggcga ggataaacat accgcgcgcc ggatcagacg caaacgcgtg taccgatacc
    36421 agcacgccgg aacgcacgag gaaagtcccc aacagacaca acgagaaggc actgattgcc
    36481 agcaataatg tccacgcttt gaagctggcg cgttgttcag tgaccgccag tgagtgcatc
    36541 agcgcagtcc ccaccagcca cggcataaac gaggcgtttt ctaccggatc ccagaaccac
    36601 cagccacccc agccgagttc gtaataggcc catgcggaac cgagcacaat gccgagcgtc
    36661 aggaaaatcc acgccgccag cgtccacggg cgagtaaaac gcgcataagt gctgtccaga
    36721 cgcccgctca gcaaagaagc aatggcaaaa gcaaacgcca ccgagaaacc cacgtacccc
    36781 atatagagca gaggcggatg gaagatcagc cccggatcct gtaacagcgg gttaagatcg
    36841 cgcccttcaa tcgggaagtt cggcagcgtg cgtgagaatg ggttagaggt aaagagaatg
    36901 aacagcaaaa agccgacgct gaccatcccc attatcgcca gtacgcgggc cacaatatcc
    36961 agcggaatac gctgactaaa aatcgccacc gcaaaggtcc agccgctcat cagcagcacc
    37021 cacagcagca gtgagccttc atgtgcgccc caggtcgccg ccacgcgata ccacaccgga
    37081 agctgggtat tggagttgct ggcaacatag gtgacggtaa agtcgttgac cacgaaagca
    37141 ttgaccagca ccagaaatgc gccagccaca gacataaaca gcagccaggc aaacaagcgg
    37201 gaagacgcca tcatgcgcgc atctccgcgc gccacgcccc atagcggata cacggacagc
    37261 agcagcgcaa tccctaacgc caggcacagc agcccgtttc caatttccgg catcatgatg
    37321 ctgggtcctt ataaacactc gccgggcgac ggtggttagc ttccatcgct ttctcaactt
    37381 ctggcggcgt gtagttttcg tcgtgtttcg ccagcacttc ttttgcgagg atatgattgc
    37441 ctttttccag ctcgccctgc accacaacgc cctgcccttc acggaacaga tccggcaaaa
    37501 tgccttcgta agagacatcc actgagcctt cagcgtcgta aatggtgaag gtcactttca
    37561 gcgaattggg atcgcgctgc acactacccg gcatcaccat cccgccaacg cgcagacgct
    37621 gaccgacttc cggcatttgc tgagtttcac gcttgccgta gagaatttcc cccggcgtat
    37681 aaaagagatc gatattcgag cgcagcgcat atagcaccag accgatagtc agcgccagcc
    37741 ctgccaacac ggcacaggca atccacaagc ggtttttacg gcgaatattc atgcagcctc
    37801 ctgctgttgc gcagcacgta aacgcgcctc acgcgcccgc tgttgcgcca cgccacgcag
    37861 aattgcgcga tgttgcatca ccgagtgcac gaccaaaacc accagcggaa taacggtcat
    37921 caccaccgcc agccagacaa aaaaggcgta accgcccatt gcgaaaaatt cattccagga
    37981 agcaaatgca ggggtcattt acggcctctt ttcagtatca gttcactcac ccacggacgg
    38041 cgtttttcca tcagcaaaat caaattacgc atccgcatca gcgtcagcgt ggcagacagt
    38101 agcaggaagc caaaaatcga ccagcgcagc ggcgaacgca tcgccggatc gatactttgc
    38161 tgcatccgcg ttgatccctg atgcagggtg ttccaccact ccacggagta atgaataatc
    38221 ggcagattca ccacgccaat cagcaccaga atacctgccg cacggcccgc cagacggcgg
    38281 tcgtcgaagg cgtgccacag ggcaatcaca cccacataca aaaacagcag caccagttca
    38341 gaagtcagac gtgcatccca tacccaccag gtgccccaca tcggttttcc ccatgcagag
    38401 ccggtaacca gggcaataaa ggtaaacacg gcaccaatgg gggccatcgc cgccaccgcc
    38461 aggttggcca ttttcatctg ccagacaagg ccaataaacg ccgccaccgc cattgatgca
    38521 taaatgccca tcgaccagat cgccgcaggc acatgcaggt agataatgcg gtagctattt
    38581 ccctgctgat aatcagccgg agcaaagccg aatccccaga tccagccgac ggtaagcacg
    38641 accacactgg caattgccag ccacggtata aaccagccac agatttgata cagccgtggt
    38701 gggatcgcca gttgatgcag tgttttccac atagtttcga taccagactc gaacaaaaat
    38761 cagtaatcca gcgttattga atgctgattc gtaacgctgc cgccgtcgca aaaggactta
    38821 atgtcgcggt gcctgccagc aacgcgccta aaattgccag atacccgtca acgggcaaat
    38881 gcatggaagc cgcgtccatc gcggcggtgg caaagatgag taatgggata gtcagcggta
    38941 acaccagtat gctgagcagc acaccaccgc gcttaagtcc cactgtcaac gccacacccg
    39001 gtgcaccgag aaagccaagc gtaggcgttc ccagcagcag cgtcagcgcc atcacttgcc
    39061 agccataaac atccattccc agtagcattg ctaccagtgg cgaaaggatg agtaacggca
    39121 gaccggttac catccagtgc gccatcacct tcgccagcac aacggcgggc aagggtaacg
    39181 gcaacaacat caattgttca agactgccgt cctgcaaatc gtcacggaac agtcgttcca
    39241 gcgccagcaa ggatgaaagc agcgcagcaa cccagataat gcccggtgca atacgcgcca
    39301 gcagttgcgg ctccggaccg atactgagcg gaaaaagggt aattacaatc aggaagaacc
    39361 acagcgggtt ggcgatttcg gcgctatggc gaaacgctac acgcagctca agacggaaaa
    39421 tgcgccagaa catcatgcgg ccctcgtttg cgtcagtgaa atgcggcgaa ttttactttc
    39481 agcaacgttg agcggctggt gggtagtcag aatcacaatc cccccctgct ccgtatgctg
    39541 cgccatacgc tgggtcagac gatcgacgcc gttaacgtca atcgcggtaa aaggctcgtc
    39601 gaggatccat aacgtggcac gggtcagcca cagacgcgct aaagcgacgc ggcgttgttg
    39661 cccggccgag agctgattta caggaatatc ttcgaatccg gcaagcccgg cctgcgccag
    39721 ggcttccaga cattgtgcgg tatcgccatc gcgatgataa aagtgcagat tttctaacgc
    39781 cgtcagccgg gttttgatcc ccggctgatg gcctatccat aacaggtttt gatggtagct
    39841 gtcgcgtacc tgatgcaagg gctgcccttg ccagagaacc tcgcctgcgt cagggcgaga
    39901 caaccccgtc agcaaacgga gaagcgttgt cttccccgcg ccgttgctac cggtgatttg
    39961 tacccactct cctgcgttca gcgtaaatga caagccacta aataaggttc gttcatcccg
    40021 ctcacaaagt aactctctgg cttcaagcat acccacgcaa taaccctgtt aaaaacctgg
    40081 ctcgacttca cgcatatcgg gcagcttgtg cgctatccct ttatggcaat caatacaggt
    40141 ttgcccatct ttcaccgcct ggtcatgcat cttcgcggca accgatttct gggcggttgt
    40201 atccatatac tcgaagttgt gacagttacg gcactcctgc gagttattgt ccttcatgcg
    40261 ccgccactca ttctgtgcca tcgtcagacg atgagcttca aatttctgcg gcgtgtcaat
    40321 aacgccaaaa attttaccat acagctcttt acttgctttg agcttgcgta tcatcttcgg
    40381 cacaaactcg tgcggaacgt gacaatccgg acaggtcgca cggacgccgc tacggttgtt
    40441 gtagtgcacg gaatccatgt attcctgata caccgtgttg cgcatttcgt ggcagctaat
    40501 gcagaactct tcggtattgg ctttttccat cccggtgtta aagccacccc agaagacgat
    40561 gccgccaaca aaaccgatca acagcagcgt ccccagcgcc agacggctgg gggtacgcca
    40621 ccatttccac aggcgcttaa tcagaccagg cttacggtca gaatttccca taataacctc
    40681 ttatttcccg taaccttttg atggggtaaa ggtattcccc acgattggcg cggtatcggc
    40741 ctgcggtacg tgacattgca gacagaaata acgacgcgga gccacttccg cgcccacttt
    40801 gccgtcgctg tccataaagt gagtaggact gatacgcggc gcgccagtgg tgcgatagct
    40861 ttcgacaccg tggcactgca agcagcggtt ggtattggtc gttacctgat aaccttcaac
    40921 gctatgcggg atcattggcg gctgattcac atagttcagc ggcatccgtt cctgctcttt
    40981 tggcatccga atggcccctt cctgtgtccc ggatacttcc ggcgactggc taaaatcgac
    41041 gccattagcc gcccaaaccg cgccgcttac caccaaggcc agcatcgccg tccattgaca
    41101 cagcgctttc ttcaggtcat ggcttttcat gatttcgctc ccgaactcca tcgtgtagtt
    41161 attgtaaata catcctcaga acagacatcc acgcagcgac cgcacgtcat gcaatcgcgg
    41221 ctggtgacct gcaccgggct ttgttcatcc agcaccgggg cacgtagcac atgcggttcc
    41281 gggcaaacat gaaaacaatc catacagcgg ttacatttct gccgatcggt ggccgcaacg
    41341 gtaatcacac ctttgctacc cagcacgcca tacagcgcgc caaccggaca aatgtgcccg
    41401 caccagccgt gttcaacgac cagtagatca aataaaaaca gcgcgagaat aagcagcgcg
    41461 ccgctgccga agcccattac caggctacgc cccatcagag aaacggggtt gatccattcc
    41521 caaatgagcg tgccagttaa ggctgatccc accagaatga ccaccaacag cacgtagcga
    41581 atgtggcgag ggatcgtcgc agactgattg aggtcaaagc gcctgcgtaa ccagttcgct
    41641 aagtcggtaa tcgggttcag cgggcagacc cagctgcaaa ataatcgctt tcccgccagg
    41701 gcgtagagca cggtgataat caccgcgccg gtcaacgcca ccgtggcagg cagatgacca
    41761 ctggcaagac tttgcagcgt catcagcgga tcggtcagcg gaacggtgtc gaataacagg
    41821 ctactgctgt agttgccgtg caagatccac acaccaaacc atggaccgct caaaaacatc
    41881 cccagcacga agaactgaca aaggcgacgt aacaccagcc aacggtgact gcgccaccag
    41941 ccttttttct ccagcgcctc gcgcccggcg tcacgtttac gatttgccat tgttcccctc
    42001 cagccagccg aagcggtaat ggtgacctaa ctcccctttc gccagtgaca gcggtaacac
    42061 cttgattgcc ggttgttcca gcacgcatac tttttcgcac ttaccgcaac cggtacaggc
    42121 gtcgctgtga accgtcggca gaaagcgggc gtgcttaccg gtacgcgtgt tgcgctccag
    42181 ctccagggtg atggcctcat caattttcgg gcattcgcga taacaaacat cgcagcgcag
    42241 cccctgaaag ttgagacagt tttcctggtc caccagtacc gccagcccca tccgcgcgtc
    42301 gtcgatcgat tcaatctcac gatccagcgc accgcttggg cacactttgg cgcacggaat
    42361 gtcctcacac atttcgcaag gaatatcccg tgcgacaaaa tatggcgtgc ccgccgacag
    42421 accagaggcc agcgtcgcca gttttaaggt gtcgtaaggg caagcctgaa cacactgacc
    42481 gcaacgcaca caggcactgg caaaggcgtt ctcgtttatg gctccgggcg ggcgcaaccg
    42541 cacgccagat gcgcgtgcgg tttgctgttg taaccccagc gccacaccca cggcagccag
    42601 cccgcctgct gtgcgaacaa catcgcgcag aaagcggcgg cgaccatttt gaggtttcgc
    42661 tgaccgggac ataatggctt acaccttctc cagtttgacc gcgcacttct tgaagtccgt
    42721 ctctttcgag agcggatcgg tcgcatccag cgtcagtttg ttaaccagct gtgcggcgtc
    42781 gaagaacggc atgtacacca ggccctgtgg cggacggtta cgaccgcgcg tttcaacaat
    42841 cgagatcact tcgccacggc gagaaaccac tttcactttg tcgccacggc gcagatcgcg
    42901 cgctttcgca tccagcgggt gaataaacaa gaccgcttcc gggaaggcgc ggtgcagttc
    42961 cggcacacgg cgagtcatac tgccggtgtg ccagtgctcc aggacgcgtc cggtagagag
    43021 ccacaggtcg tactcttcat ccggtgcttc cgccgccggt tcgaacggca gcgcgaagat
    43081 caccgctttg ccatccggtt taccgtagaa cttgtagcct tcgcccgctt tcacgtacgg
    43141 gtcgttacct tcgctgtaac gccactgcgt ttctttaccg ttcaccaccg gccagcgcag
    43201 accgcgcgct ttgtggtagt catcgaacgg tgccagatcg tgaccgtgac cgcgaccaaa
    43261 ccatgcgtac tcttcgaaca gccctttttg cagatagaag cccagctcgc gggattcatc
    43321 gttcagctga tcttccgcca gttcggatac cgggaatttg ctcacttcgg gggtggcata
    43381 cagaacttcg tacagcgttt tgccacgcag ttccggtttc ttcgccagca gctcttccgg
    43441 ccatacttct tcagttttga agcggcggga gaactggact aactgccaga gatccgattt
    43501 cgcttcgccc ggtgcctgta cctgctgacg ccagaactga gtacggcgtt cggcgttacc
    43561 gtaagcgccc tctttctcta cccacattgc ggtcggcagg atcaagtcgg cggccagcgc
    43621 actgactgtc ggatacggat cggagacgat gatgaagttg cgcggatcgc gccagcccgg
    43681 catacgctct tcattgatgt tcggcccggc ctgcatgttg ttggtacaca tggtccagta
    43741 aacattcagc ttgccgtctt tcagcgcacg gtcttgtgct accgcatgca gaccgatttt
    43801 cgccggaatg gtgccgctcg ggatattcca cttcttctcg cagatatcac gatgtttctc
    43861 gttagtcacc accatgtccg caggcagacg gtgagcaaag gtgcccactt cacgcgcagt
    43921 accacacgcg gaaggctgcc cggtcaggga gaacggaccg caacccggct gggaaatttt
    43981 gccggtcagc aggtgcaggt tgtagaccag gttgttggcc cacacgccac gagtatgctg
    44041 gttgaagccc atcgtccagt aggagatgac tttcttgttc ggatcggcat acagctgcgc
    44101 cagttgttct aactggtctt tcggcacgcc ggtcatttcg gcagtttttt ccagcgtata
    44161 ctcggcaacg aaggctttgt aatcttcaaa gctcatcggt tcggaggcgt cagaacccgg
    44221 attcttcgct gctttttcca gcggatgggt cggacgtaaa ccgtagccga tgtccgtcgc
    44281 ccctttgcgc aggttaacgt gcttgctgaa gaagtcctga tttatcgcat tgttttgaat
    44341 gatatagttg gcgatgtagt tcaggatcac caggtcagat tgcggcgtaa agatgatgcc
    44401 gttatccgcc agctcgaagc tacgatgctg gtaggtagaa agcaccgcca cggtgacgtt
    44461 ctggttagag agacgacggt tagtgatgcg tgaccagagg atcgggtgca tctccgccat
    44521 gttagcgccc cacagcacaa acgcgtcagc ctgctcgatg tcgtcatagc agcccatcgg
    44581 ctcatccata ccaaaggtac gcataaagcc aactactgcc gacgccatac agtgacgtgc
    44641 gttcgggtcg atgttgttcg aacggaagcc cgctttgaac agcttggacg cggcataacc
    44701 ttcccagata gtccactgac cagaaccgaa cataccgatc gattccggtc ctttttcttt
    44761 cagggcggtt ttgaatttct cttccatcac atcgaaggcc tgatcccagg tgattggggt
    44821 aaattcgcct tctttgtcat atttaccgtt tttcatacgc agcagcggct gcgtcaaacg
    44881 gtctttaccg tacatgatct tgggcaggaa atagccctta atgcagttca ggccacggtt
    44941 aaccggtgcg tccgggtcgc cctgacaggc caccacacgc ccctgctgcg ttccgaccag
    45001 aacgccgcaa ccagtaccgc agaaacggca cggcgcttta tcccatttaa tggcttcctg
    45061 ctgaccaaca acggcgcggg caacgcccgg cacgctgaga ccggcagccg ccgcagcggc
    45121 cgcaacggcg ttagctttca taaagctacg acgactgagt ttcatggtgt ttcctcacct
    45181 tgctcttcct gctggtgata aaccagcgac accgccagca cgccctctac gttgcgtact
    45241 gactcaatgg tttggatcag cgtttcgctg tcttctgctt ccaccaccac aatcaactga
    45301 ccgctcggcg cgtcgctgac agcaacttca cagccgggaa aggcgttcag ttgggtgctg
    45361 atgtctgaaa ttcgttcgct tttggcctgc acgaccaggc tgcaaacttg ccagttagtg
    45421 tgcatggaga tactccgcag ttatggctga taccggacag ctggctgcac atgctccaca
    45481 tccgttgcaa agttggctat taagttgcgg ctggtagatt ccggagagcg tcgggcgaaa
    45541 gataattgcc attggctcac agctatcctg acagcggcgg cattcaactg actgatacgc
    45601 caggcacgcg tccccaatgg tgaactgcaa atcccaggcc ctggtgtggc gcggagaaaa
    45661 tagcgattcg gggcaagcct gcgcgcaggc gtaacagaag ctgcactcgt tatttttgaa
    45721 attaacgctc ggatagccgc ccgcgccgcg ttgcagaatg ttgttttcac aagcattaat
    45781 acaggcgtcg caacgggtgc aatgggtcag aaaatgagat tcatcaccgg accagggcgg
    45841 acggataccg ttactggctt tgcgccagcg accagtgagt atgccccgac gggatgcatc
    45901 aatcttcaca ttgaccttcc atcattaacg cgctctgaaa attgagagcg accaaataaa
    45961 ccgcataatt aataagccat ttttatagcc gctaagatat taaaggatgt gtcaaagatg
    46021 cataccccga tcggggtaaa acctgtagca ggatcaaaag agtggcggga agcgtggcaa
    46081 aaacgggctt ttgctcacat ttcaaatggt tataaacata tttatatagc gattaattca
    46141 ccagagatat ttctgctggt ttgccccctc atcagaattt aacactaaaa gagcaggtaa
    46201 aattgtctga atgttcttta agttattcat aaagcaaatt aataaatctg atgaatatgt
    46261 taaccttcag cgacatcatc ggtgaaaacc tataaatgaa gaaggaaagc aaaaaaatga
    46321 agaccattct acctgcagta ttgtttgccg ctttcgctac cacttccgcc tgggcggcag
    46381 aaagcgtcca gccactggaa aaaatcgcgc cttatccaca agctgaaaaa gggatgaagc
    46441 gtcaggtgat tcagttaacc ccgcaagaag atgaatctac cctgaaagta gaactgttaa
    46501 tcggtcagac gctggaagtc gattgcaatt tgcatcgtct cggcgggaag ctggaaagca
    46561 aaacgctgga aggctggggc tatgattatt atgtctttga taaagttagc tcgccggttt
    46621 ccacgatgat ggcctgcccg gatggcaaga aagagaagaa atttgtcacc gcgtatctgg
    46681 gcgatgctgg aatgctgcgt tacaacagca agctgccgat cgtggtgtat acgccagaca
    46741 atgtagatgt gaagtaccgc gtctggaagg cggaagagaa aattgacaac gcggtagttc
    46801 gctaaactgc cgtgaagtgc ggcaccccgt aggtcagaca aggcggtcac gccgcatccg
    46861 acatccaacg cccgagccgg ttgcctgatg cgacgctggc gcgtcttatc aggcctacac
    46921 cgctgtgaag tgctccaccc cgtaggtcgg ataaggcggt tacgccgcat ccgacatcta
    46981 acgcccaagc cggttgcctg atgcgacgct ggcgcgtctt atcaggccta caccgctgtg
    47041 aagtgctcca ccccgtaggt cggataaggc ggttacgccg catccgacat ccaacgtccg
    47101 agccggttgc ctgatgcgac gctggcgcgt cttatcaggc ctacaccgct gtgaagtgct
    47161 ccaccccgta ggtcg
//
LOCUS       DAJOWB010000024        46844 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJOWB010000024 DAJOWB010000000
VERSION     DAJOWB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.024"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(143..685)
                     /locus_tag="OHO85_001507"
     CDS             complement(143..685)
                     /locus_tag="OHO85_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001502553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCQ2670900.1"
                     /translation="MIYPTNTGKSGEHLRLATLESVWIQGKLRMWGRWSYIGGGRSGN
                     MFNQLLASKKLTKTAINEALRRMKKAGIEKAELEAFLREMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFSTCEKRIANWL
                     AVAEYALYIPMRESFAQKTA"
     gene            complement(682..972)
                     /locus_tag="OHO85_001508"
                     /pseudo
     CDS             complement(682..972)
                     /locus_tag="OHO85_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
     gene            complement(972..1571)
                     /locus_tag="OHO85_001509"
     CDS             complement(972..1571)
                     /locus_tag="OHO85_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCQ2670901.1"
                     /translation="MAHIQLVKQTSSGLLLTATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPAGGAITPRERELVSGFVDYLCDSVGREHTPALSDAAEQ
                     YLNTVATRRTRDTALLKSFDAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     EFQQVYKAVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
     gene            complement(1643..1855)
                     /locus_tag="OHO85_001510"
     CDS             complement(1643..1855)
                     /locus_tag="OHO85_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670902.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
     gene            complement(2131..2286)
                     /gene="hokD"
                     /locus_tag="OHO85_001511"
     CDS             complement(2131..2286)
                     /gene="hokD"
                     /locus_tag="OHO85_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCQ2670903.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(2829..3392)
                     /locus_tag="OHO85_001512"
     CDS             complement(2829..3392)
                     /locus_tag="OHO85_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670904.1"
                     /translation="MTELTKEQLIEEAKLKIAIAKCHPNSGLARVEGELFKIALASLE
                     AEPIAWECGENDSCLTLTQLKHTRNCAEITPKPLFTATPVPLTPEGLVKAVRFYEQVK
                     RENPPVETGAWKDAVDWVLKEACCAVILGKADNPLASSNQVGELTMLVKRLVSQLKKA
                     NPDCKLPEMAMDYLKRNELISAEDVLR"
     gene            complement(3539..4012)
                     /locus_tag="OHO85_001513"
     CDS             complement(3539..4012)
                     /locus_tag="OHO85_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCQ2670905.1"
                     /translation="MSAPATILDMCCGSRMFWFDKNDDRAIFSDIRKEEHTLCDGRRL
                     IISPDLIADFRALPFADASFSMVIFDPPHLERVGDNAWMGKKYGRLNKDTWRDDLRQG
                     FKEAFRVLRPSGVLIFKWNETQIPVRQILILTDRKPVIGQRTGKNDKTHWIIFMK"
     gene            complement(4009..4431)
                     /locus_tag="OHO85_001514"
     CDS             complement(4009..4431)
                     /locus_tag="OHO85_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCQ2670906.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKTVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEVAQ"
     gene            complement(4447..5208)
                     /locus_tag="OHO85_001515"
     CDS             complement(4447..5208)
                     /locus_tag="OHO85_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCQ2670907.1"
                     /translation="METVFDALKAMGKATSIELAARLDISREEVLNELWELKKAGFVD
                     KSAYTWRVADNNVQQEQPAQAELPEETTTATVAKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKFRYCIPGDNLPAEPKAASVTETDGKAFPQ
                     PAGVALPVQEAATQEDIKTETVADIVQSLPSFTETRADDLILPSLHMANRELRRAKSH
                     VQKWARVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            complement(5231..5977)
                     /locus_tag="OHO85_001516"
     CDS             complement(5231..5977)
                     /locus_tag="OHO85_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCQ2670908.1"
                     /translation="MKNIATGGVLERIRRLAPPHVTAPFRTVAEWREWQLAEGLKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVVQPGIAK"
     gene            complement(5984..7114)
                     /locus_tag="OHO85_001517"
     CDS             complement(5984..7114)
                     /locus_tag="OHO85_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670909.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNADCNARGVTKSAIDRITFMAGFADALIQVGWLVENDGGLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAGSVTQTDQKALPEEEEEEDINTDLTHPKPFPSGRE
                     FRDFVAGVLEGRLSGGTAAEFCNSAVAALQAAGLDVCREYPVPERGDGCGGRIDIVVT
                     DRNGVRCGIELDRNSPRQKSLLKIGAVETGICVLRRSDIARHTEQGILVIGGAVRQKK
                     FDPLSVALPDWLPETLWHEWVQFRQALRKPIRTEHGANGAIRELEKFRQQGFSPEQVI
                     RHSIANEYQGLFAPKGVRPETLLRQVNTVSLPDSAIPPGFRG"
     gene            complement(7192..7614)
                     /locus_tag="OHO85_001518"
     CDS             complement(7192..7614)
                     /locus_tag="OHO85_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670910.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            complement(7611..7853)
                     /locus_tag="OHO85_001519"
     CDS             complement(7611..7853)
                     /locus_tag="OHO85_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2670911.1"
                     /translation="MNKISTYRKQLGLSQRQLAVQLGWIQSRLANYEANFRTPGLEEC
                     RKIVSTLNRLGAHCGLDDVFPPDGKHSENSIGAVDS"
     gene            7951..8340
                     /locus_tag="OHO85_001520"
     CDS             7951..8340
                     /locus_tag="OHO85_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2670912.1"
                     /translation="MKTIAEQIGERLKATRLNRGLSMGNLAKLCGWSGSSRIANYEAG
                     TRSVGAEDAIILGQVLGISPAELMFGEQENTYSWLNDNQKKMLELFNQLPGSEQARMI
                     DLFEIRLQELDEYVEKYLRSRQQKKSS"
     gene            8651..8806
                     /locus_tag="OHO85_001521"
     CDS             8651..8806
                     /locus_tag="OHO85_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCQ2670913.1"
                     /translation="MDTIYLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            8808..9011
                     /locus_tag="OHO85_001522"
     CDS             8808..9011
                     /locus_tag="OHO85_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670914.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            9692..9913
                     /gene="kilR"
                     /locus_tag="OHO85_001523"
     CDS             9692..9913
                     /gene="kilR"
                     /locus_tag="OHO85_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HCQ2670915.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            9907..10083
                     /locus_tag="OHO85_001524"
     CDS             9907..10083
                     /locus_tag="OHO85_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2670916.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            10158..10434
                     /locus_tag="OHO85_001525"
                     /pseudo
     CDS             10158..10434
                     /locus_tag="OHO85_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10534..13680
                     /gene="recE"
                     /locus_tag="OHO85_001526"
     CDS             10534..13680
                     /gene="recE"
                     /locus_tag="OHO85_001526"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCQ2670917.1"
                     /translation="MSTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRG
                     AVAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIAPS
                     SSAQDKPASTDNTNVNGEDMTEIEENMLLPISGQELPIRWLAQHGSEKPVTHVSRDEL
                     QTLHIARAEELPAVTALAVSHKTSLLDPLEIRDLHRLVRDTDKVFPNPGNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLA
                     RDVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFIAMPGGLDYSRAI
                     VVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEE
                     EKQDDEEKPQPSCAMADEQATAETVEPDATEHHQDTQPLDAQSQVNSVDAKYQKLRAE
                     LHEARKTIPPKNPVDADKLLAASRGEFVEGISDPNDPKWVKGIETRDSVNQNQQESEQ
                     NDQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCNACGQSGGDNCPDCGAVMGDATYQ
                     ETFDDENQVEVRENEPEKMEGAEHPHNENADSDPHRNCSDETGEASAPVATEIMWPSY
                     FEPGRYENLPNEVYHSANGISSTMLKDARISLMYYHGRHIAGTIPNEESDALLRGRII
                     HSYVLETDKFADEYAIPVPVPEYVVTTSNELIAIIKKHNASLPALMTPEQMKEWIESY
                     NSTLIQPLSVSAGAEETGILYGSLPVEFRRIPEGEKHTASAMKACIKEYNASLPPLLK
                     TSGAREQLLDQIETVDPELAKKERAKSLPYNISGTKEQLTEIARKIRPELVTLEDWQK
                     RQQEENAGKTFISPDMYEQAKNIHAALQNNTDAARLLNHPDRKSEISYFGFDEETGLE
                     IRVRPDIEIRLPYESICADVKSVSLGYVRQERLKDRLHREIIERDYHLSAAMYCDVAN
                     LDKFFWIFVNKDAGYHWVAVVEASQELLELGRQEYRRTLHQINEALETNNWPAPITES
                     YTDELNDFDLRRLEALSI"
     gene            13692..14744
                     /locus_tag="OHO85_001527"
     CDS             13692..14744
                     /locus_tag="OHO85_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HCQ2670918.1"
                     /translation="MNELTQQENINSNVAVFSPQSLAAIQTFSQVMASGMATVPEHLR
                     GNPSDCMAITMQAMQWQMNPYAVAQKTFVVNGVLGYEAQLVNAVISTRGPLTGRIEYD
                     WFGPWEKIIGKFEIRKNDKGKEYRVPGWKLVDENGIGVRVQATLRGESKPRVLELLLA
                     QARTRNSTLWADDPRQQLAYLALKRWARLYCPEVILGVYTRDELDEPQEKIINPVQEH
                     KNTSACRAERETTIIEQDVGENWISAFRERIEQAQSTGETTALRQEVEEHKNTLGALY
                     TELKGKVVQRHHRLNAIARIEKMINDLPSSGDPEAEQKFTALENTLNAARPHLGELYE
                     AYKTTLTDMKPEYIGS"
     gene            14808..15002
                     /gene="ralR"
                     /locus_tag="OHO85_001528"
     CDS             14808..15002
                     /gene="ralR"
                     /locus_tag="OHO85_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
                     /protein_id="HCQ2670919.1"
                     /translation="MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYSGSEK
                     EALARWNKRTTGNNNGGVHV"
     gene            14995..15183
                     /locus_tag="OHO85_001529"
     CDS             14995..15183
                     /locus_tag="OHO85_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HCQ2670920.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLLSS"
     gene            15290..15571
                     /locus_tag="OHO85_001530"
     CDS             15290..15571
                     /locus_tag="OHO85_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCQ2670921.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLLEKLKHAAEKIRSQFA"
     gene            15537..16613
                     /locus_tag="OHO85_001531"
     CDS             15537..16613
                     /locus_tag="OHO85_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCQ2670922.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     IAQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRGN
                     QLETIKEKLGGILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGAVVERCRMVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKLTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(17006..17347)
                     /locus_tag="OHO85_001532"
     CDS             complement(17006..17347)
                     /locus_tag="OHO85_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCQ2670923.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(17360..18232)
                     /gene="copD"
                     /locus_tag="OHO85_001533"
     CDS             complement(17360..18232)
                     /gene="copD"
                     /locus_tag="OHO85_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HCQ2670924.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(18236..18610)
                     /gene="yobA"
                     /locus_tag="OHO85_001534"
     CDS             complement(18236..18610)
                     /gene="yobA"
                     /locus_tag="OHO85_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCQ2670925.1"
                     /translation="MVSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            18749..18979
                     /gene="holE"
                     /locus_tag="OHO85_001535"
     CDS             18749..18979
                     /gene="holE"
                     /locus_tag="OHO85_001535"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCQ2670926.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            19081..19737
                     /locus_tag="OHO85_001536"
     CDS             19081..19737
                     /locus_tag="OHO85_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCQ2670927.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASTRR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            19761..20423
                     /gene="exoX"
                     /locus_tag="OHO85_001537"
     CDS             19761..20423
                     /gene="exoX"
                     /locus_tag="OHO85_001537"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCQ2670928.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(20420..22480)
                     /gene="ptrB"
                     /locus_tag="OHO85_001538"
     CDS             complement(20420..22480)
                     /gene="ptrB"
                     /locus_tag="OHO85_001538"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCQ2670929.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWIFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFYAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFKEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(22689..23348)
                     /locus_tag="OHO85_001539"
     CDS             complement(22689..23348)
                     /locus_tag="OHO85_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCQ2670930.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(23675..24031)
                     /locus_tag="OHO85_001540"
     CDS             complement(23675..24031)
                     /locus_tag="OHO85_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCQ2670931.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(24098..24388)
                     /gene="yebG"
                     /locus_tag="OHO85_001541"
     CDS             complement(24098..24388)
                     /gene="yebG"
                     /locus_tag="OHO85_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HCQ2670932.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            24522..25700
                     /gene="purT"
                     /locus_tag="OHO85_001542"
     CDS             24522..25700
                     /gene="purT"
                     /locus_tag="OHO85_001542"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCQ2670933.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHIINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(25756..26397)
                     /gene="kdgA"
                     /locus_tag="OHO85_001543"
                     /gene_synonym="eda"
     CDS             complement(25756..26397)
                     /gene="kdgA"
                     /locus_tag="OHO85_001543"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCQ2670934.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEVGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(26434..28245)
                     /gene="edd"
                     /locus_tag="OHO85_001544"
     CDS             complement(26434..28245)
                     /gene="edd"
                     /locus_tag="OHO85_001544"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCQ2670935.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEVGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKISLVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(28480..29955)
                     /gene="zwf"
                     /locus_tag="OHO85_001545"
     CDS             complement(28480..29955)
                     /gene="zwf"
                     /locus_tag="OHO85_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCQ2670936.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            30293..31162
                     /gene="hexR"
                     /locus_tag="OHO85_001546"
                     /gene_synonym="yebK"
     CDS             30293..31162
                     /gene="hexR"
                     /locus_tag="OHO85_001546"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCQ2670937.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            31290..32732
                     /gene="pyk"
                     /locus_tag="OHO85_001547"
     CDS             31290..32732
                     /gene="pyk"
                     /locus_tag="OHO85_001547"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCQ2670938.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(32863..33834)
                     /gene="lpxM"
                     /locus_tag="OHO85_001548"
                     /gene_synonym="msbB"
     CDS             complement(32863..33834)
                     /gene="lpxM"
                     /locus_tag="OHO85_001548"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCQ2670939.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(33954..35276)
                     /gene="mepM"
                     /locus_tag="OHO85_001549"
     CDS             complement(33954..35276)
                     /gene="mepM"
                     /locus_tag="OHO85_001549"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCQ2670940.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(35292..36224)
                     /gene="znuA"
                     /locus_tag="OHO85_001550"
     CDS             complement(35292..36224)
                     /gene="znuA"
                     /locus_tag="OHO85_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCQ2670941.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            36303..37058
                     /gene="znuC"
                     /locus_tag="OHO85_001551"
     CDS             36303..37058
                     /gene="znuC"
                     /locus_tag="OHO85_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCQ2670942.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            37055..37840
                     /gene="znuB"
                     /locus_tag="OHO85_001552"
     CDS             37055..37840
                     /gene="znuB"
                     /locus_tag="OHO85_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCQ2670943.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(38084..39094)
                     /gene="ruvB"
                     /locus_tag="OHO85_001553"
     CDS             complement(38084..39094)
                     /gene="ruvB"
                     /locus_tag="OHO85_001553"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCQ2670944.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(39103..39714)
                     /gene="ruvA"
                     /locus_tag="OHO85_001554"
     CDS             complement(39103..39714)
                     /gene="ruvA"
                     /locus_tag="OHO85_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCQ2670945.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATNDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(39853..39918)
                     /gene="yobI"
                     /locus_tag="OHO85_001555"
     CDS             complement(39853..39918)
                     /gene="yobI"
                     /locus_tag="OHO85_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCQ2670946.1"
                     /translation="MYIFITHFFTEYVILKYLLPK"
     gene            39989..40591
                     /locus_tag="OHO85_001556"
     CDS             39989..40591
                     /locus_tag="OHO85_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCQ2670947.1"
                     /translation="MNINYPAEYEIGDIAFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(40593..41114)
                     /gene="ruvC"
                     /locus_tag="OHO85_001557"
     CDS             complement(40593..41114)
                     /gene="ruvC"
                     /locus_tag="OHO85_001557"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCQ2670948.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(41149..41889)
                     /locus_tag="OHO85_001558"
     CDS             complement(41149..41889)
                     /locus_tag="OHO85_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCQ2670949.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(41918..42370)
                     /gene="nudB"
                     /locus_tag="OHO85_001559"
     CDS             complement(41918..42370)
                     /gene="nudB"
                     /locus_tag="OHO85_001559"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCQ2670950.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(42488..44260)
                     /gene="aspS"
                     /locus_tag="OHO85_001560"
     CDS             complement(42488..44260)
                     /gene="aspS"
                     /locus_tag="OHO85_001560"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCQ2670951.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEDILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            44570..45136
                     /locus_tag="OHO85_001561"
     CDS             44570..45136
                     /locus_tag="OHO85_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCQ2670952.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWSAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILNAL"
     gene            45133..45627
                     /locus_tag="OHO85_001562"
     CDS             45133..45627
                     /locus_tag="OHO85_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCQ2670953.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIF
                     IRHV"
     gene            45680..>46844
                     /locus_tag="OHO85_001563"
     CDS             45680..>46844
                     /locus_tag="OHO85_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCQ2670954.1"
                     /translation="MKRVISYIRFSSEKQAKGDSLRRQSKMVTDWLKAHPEYYLDQDL
                     SFRDLGVSGFTGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQAVDRASELLR
                     NILRSGVDVVTLSDGEHYTRESLRDPLALIKSILIMQRAHEESLRKAERIQAAWDAKK
                     EAAKAGIKVSKRCPAWLKLNDDRKSFSIIPEKVETVKRCFQLRLEGKSIWEIVRTLND
                     EGHQSMSQYKPGKFGQTSVQDLLKNRSVIGYFTPKGCEEISGYYPAIIDETTFYKVQQ
                     LSYARYGRKPASDKPLSVNLFKGVIRCSECGHALIITGFSDTRSGIYRCPLRNENRCS
                     AKGLSRKQTDGTLLALLSRLDRFDSDTTNTIDMLRLHREHISSNIEKLVQLSLE"
BASE COUNT        11917 a        11903 c        11192 g        11832 t
ORIGIN      
        1 taaatgtcgt ttcttatatc acaccacaaa aacaacaaaa cccgctcgat gcgggttcta
       61 ttaaagttta attgcgcttg attcgccacg cgatacagct ttgcgaagcg tagcaaaatt
      121 gaagcagttt atgcgtaaaa aatcaagccg ttttttgagc gaatgattct cgcatgggaa
      181 tgtatagcgc atactcagca acggccaacc aattagcaat tcgcttttcg catgtgctaa
      241 aacaccactc tgggtgtgca tcatttagca attcagccat tttgcgtttg gtcatccccc
      301 gcccctcata ccgttgccga aggacgctaa tcaatcctgg atgctctgcc agcacctcac
      361 ttatgactcg atcaatacat aacgcctctg catcagtaca atgcgccagc cagctctttt
      421 gcttgccgtt gatcatctct ctcaaaaacg cttccagctc agctttctct attcccgctt
      481 ttttcattct gcgcagggct tcattgatgg ctgttttcgt caattttttg gatgccaaca
      541 actgattgaa catatttcct gacctgccac cgccaatata cgaccagcgc ccccacatac
      601 gcagtttgcc ctgaatccag acactttcca gcgtggcgag acgaaggtgt tctccgcttt
      661 ttcctgtatt cgttgggtaa atcataaata accttccttt ctccagattt cttgtgtgcg
      721 aaaaacacct tctgcatgca tcaggcgtaa ttcttctttg gtgtaatcgc tggtttttac
      781 ccgcccgtcg attaaatcgt ggcacgagct acaggcaatc gctgcctgca tatcgtgtgg
      841 ttttatcgcc gctccacacg ttcccgccag tcggtaatgc gccagcacag acgtttcagg
      901 attgtgattg cagtatccag ggattctgac ctgtcacatc tggccccgcg ccgctttacg
      961 taaattcacc attacgcaaa ctccagcagc tgcgcggcca cattttcgac ttcctccgga
     1021 gaggaaaatt tacgaaacag gatccagttc cacagcacat tcagtacggc tttataaacc
     1081 tgctgaaact cggtttcgtc catattcgca aaagcaatgg atttcgcccg acgcccgcgg
     1141 ctaccgtccg gataaaaatg ctcggtgtaa aatccggcct gaatggttac ccattcgcgg
     1201 aaagcgtcaa atgacttaag caatgccgta tcccgggttc tgcgtgtcgc aacagtattc
     1261 agatattgct ctgcggcatc actcagagct ggcgtatgtt ccctgcctac cgagtcacac
     1321 aggtaatcaa cgaaaccgga caccagttct cgttcgcgag gcgtgatcgc cccaccggcc
     1381 ggagtccagt aatcgaatcc cagttgcagg agtttgaaaa aacgcttatg gaatgcgtag
     1441 ttacgcacac gcttaaagtc tgcgtgtatc cactcgccta tttttatttg atgcaaaaaa
     1501 tcgcaactct ccggcgtcgc cgtgagaagt aatccggaag aggtttgttt gaccagttgt
     1561 atatgcgcca tcgtagttct ccgctggcgc agtagaatgg gtgttcagcc cgttatgtag
     1621 tataccagaa ttaatgccaa tactaacagg atgctctgac tcgcaattca tccagcagtt
     1681 tatcatttcc cataatgtca cttaccctca tcggtaaaaa aattgccttt cgaccattac
     1741 gatacatcat tgattttttg gtttcaggga agtaatccat ttcgactata actgacaggt
     1801 catcacgacg tatgactgca tatttgctac taaatagttt ctttattttt tccacgatgc
     1861 ctccaggttt ataagtacaa acggttatat tcacatagag acaaaaatat taatctgaaa
     1921 aatatttatt tcacgtcgta tatttgatta tttaatgtgc aggtgcaatg acttttattt
     1981 tttgttgtgt atataatcaa atatatggtt atttttcacc ctgcgcattc agcgcgcaac
     2041 aaaaaacccg ccgaagcggg tttggtgcgg gtgcgttgag gatgcctgac acatgagagg
     2101 tggcgaggga tttctccccc gcctggtctc ttactcctca ggttcgtaag ctgtgaagac
     2161 agcgacctcc gtctggccgg ttcggattcg aacctcgcag aggtctttcc tcgttaccag
     2221 tgccgtcaca atgacggtta aacagatgac gatcagggcg attaacatcg ccttttgctg
     2281 cttcatagcc tgcttctcct tgcctttcgg cacgtaagag gctaacctac gtttgtgaag
     2341 catagattgg gcctcagatt aatgttaaac gtcttgcagg acgcgtaatg ttaactgggg
     2401 cttttctcta tctgccgttg gtgttcatgc ccgaggcaga tagcctcaag cacccgcagc
     2461 cattctactt aactcccatc acctcgccaa tatgaaatca atcagagagg tgattcataa
     2521 gaacgataac aagacaataa attgccatta ctgccgcaac agccagcgca catttcagaa
     2581 ccagcacgat gacctcccat atttgacgta cacgcgcatg attcaatatg tagcaaccta
     2641 tttcttgctt caatataaaa tcaggtattg ttgtataact atttaactaa ctccctatcc
     2701 ccacatctca ggcgatcaac atctgtgtac ggggcgtttt ttaatacctt acccccagcg
     2761 gcaaatcgaa tacaccacca gcgccaccgc cattgcaatt cctaccgttg tgaatgcttc
     2821 aggccaggtc atcgcaaaac atcctccgcg cttatcagtt cgttccgctt caggtagtcc
     2881 atcgccatct ccggtaattt gcagtctgga ttagcttttt tcagttgact gaccagtcgt
     2941 ttaaccaaca ttgttaattc gccaacctga ttactggatg ctagtggatt gtcggcttta
     3001 cccagaatga cagcacagca ggcctctttg agtacccaat caacagcatc tttccatgct
     3061 cctgtttcga ctggtggatt ctcacgcttt acctgttcat aaaagcgcac ggctttaacc
     3121 agtccttctg gtgtcagtgg cacaggcgta gcggtgaata gaggtttagg tgttatctct
     3181 gcacagtttc gcgtatgctt caactgtgtc agggttaaac aggaatcgtt ttcgccgcat
     3241 tcccacgcta tcggctctgc ttccagcgat gccagagcaa ttttgaataa ctcaccctct
     3301 actcgtgcca accctgaatt ggggtggcat ttcgcaatcg ctatttttaa tttggcctct
     3361 tcgattaatt gctctttggt taattcagtc atttttcact accgcccttt cgggcggcct
     3421 cctgatgatt tgagggtgca gaaatccctc cgattaagga ttaaatttta tttacagaac
     3481 taaatttaat tattcagata tacgtatctg taaccttacg aacctactca ctggatgcct
     3541 atttcataaa aataatccag tgggttttat cgttttttcc tgttcgttga ccgataacag
     3601 gttttctgtc ggtcagtatc aatatctggc gaacaggtat ttgcgtttca ttccatttaa
     3661 aaatcagaac gccggatgga cgcaacacac gaaaggcttc tttaaatccc tgccgcaaat
     3721 catcacgcca ggtatcttta ttcagccgtc catatttctt tcccatccag gcgttatcac
     3781 caacacgctc aagatgcgga gggtcgaata taaccatcga aaaagatgcg tctgcaaatg
     3841 gtagtgcacg aaaatcagct atcagatcag gactgataat caagcgtcgt ccatcacaca
     3901 atgtgtgctc ttcctttctg atatcgctaa atatcgcccg gtcgtcattc ttatcgaacc
     3961 agaacatgcg actgccacag cacatatcaa gaatggttgc cggtgcactc actgcgctac
     4021 ctcctgaaaa ttaccctgat agaaagccag tacacgctgc atagcttcgc tcttccggca
     4081 ctcgcgacag attatattca ggcgcctgtc gtagcggcgt atttctccgt ctggtaatga
     4141 ccagataagg tcaggatcaa ccacaaccgt ttttttcacc tttgccctgg agagtttttt
     4201 gcgggcgttt tgccagtcct tacgggcctg ttcagacgga aataacccgt aaccagagtt
     4261 gtatacatcg ccactggcaa caagctctct ggcgagaacg ctcatcagat atcttgtcgc
     4321 acctgtcttg gcttccagtt gccgtaacgt ctcgcgccca ctccggcgta ctagctcaac
     4381 aacctgccct ttaatttttt cccgctcttc ttgtgtaaat acttttgcca taagtgcctc
     4441 cggcaatcac ttttccgata caacacggcg ggaagaatca gtaatctgtc gaacaatatc
     4501 ccggtgcttg ttcagctccc gcagcgcggc gcagactcgc gcccacttct ggacatgact
     4561 tttcgcccga cgcagttcgc ggtttgccat atgcagcgat ggtaaaatca ggtcatccgc
     4621 tcgcgtttcg gtgaacgatg gcagcgactg cacaatgtcc gccacagttt ctgttttaat
     4681 atcttcctgt gttgccgctt cctgtactgg taacgcaaca cctgcgggct gaggaaaggc
     4741 cttaccatca gtttccgtta ccgatgcagc tttcggctct gctggtaaat tatcgcccgg
     4801 tatgcagtaa cgaaatttac cgttctgatt tacgcgaatc agacgcccct tgctgattgc
     4861 cattgccagc gttgaagcta ctttgcgtga tgtggtacca aacaatgtag ccagctcatc
     4921 agccgtttgt ggtccgcgtt gttcaatcgt tgcggttaaa tcgcactctg agattttcgc
     4981 tactgttgct gtggtggttt cttccggcag ttctgcctgc gctggctgtt cctgctgaac
     5041 gttgttatca gccacacgcc aggtgtacgc gcttttatca acaaaaccag cctttttcag
     5101 ttcccatagt tcgttcagca cttcttcacg actgatatca agtcgcgcag caagttctat
     5161 ggatgtggct tttcccattg ctttcagtgc gtcaaaaaca gtctccatta aaatttcctc
     5221 ccggtaaaaa ttacttcgca attcctggct ggacgacatt cggacgccag ctctcccagt
     5281 taaaattcac ccatcgcccg ccgttcatgg tcatgcgatc cataatcctc tcgccgagca
     5341 atgttttcat ggcctcatag ttcaggtttg tcagcatccc cacgctgcgc atagacgctg
     5401 tccggcgatc aacaatttgg tgcagcacca cctgctcgtt ttttgtctcg cgctgaatgc
     5461 caatttcatc aagaaccagc agatccactt cgcacagttc ccgcaaaaat ttttcacctg
     5521 attgcccgtc gtcatagctg gcgtgtagag cactcatgac atcagccacg gtaaccacaa
     5581 tcacggtctg gccatctttc agcaggcgat tcccgatagc cgccgccaga tggttttttc
     5641 cggtaccagg ttttccgctg aacgcaaaat ttgtacaccc ggtcatcagt tcatcagcga
     5701 tagatttcgc ctggctcaac gcgtatcgct gaccgtcgtt ctgcacctgg taattcgcaa
     5761 acgagcattt acggtgcaac ggctggatgc ctgagcgatt cagaattttt tccacccgca
     5821 actggcgatt caggcggttg atctcctcgc tacgtttcag gccttcagca agttgccact
     5881 ctcgccactc cgctaccgtt ctgaatgggg cggttacatg tggcggggcc agtctgcgga
     5941 tacgttcaag cacgccgcct gtcgcaatat ttttcatggt cagttacccc ctgaagcctg
     6001 gcgggatcgc actatccggt aacgagacgg tgttaacctg tcggagtaac gtctcaggtc
     6061 gaacaccttt cggcgcgaac aagccctggt attcattggc gatgctgtgt cgaatcacct
     6121 gctcaggtga aaaaccctgc tggcggaatt tttccagctc ccgtatcgcc ccgttagcgc
     6181 cgtgctccgt tcgaattggt tttcgcaatg cctgcctgaa ctggacccac tcatgccaga
     6241 gtgtttccgg caaccaatcg ggcagagcaa ctgacaacgg gtcgaatttt ttctggcgaa
     6301 ccgccccacc gataaccaga attccctgct cggtgtgcct tgcgatatca ctgcgccgca
     6361 agacacatat cccggtttca acagcaccga ttttgagcaa tgatttttgt cgtggagaat
     6421 ttcggtcaag ttcgatccca caccggacac cgttcctgtc agtcacgacg atatcaatcc
     6481 gccctccgca gccgtctcca cgctctggta ccggatactc acgacagaca tccaggccag
     6541 cagcctgcaa cgccgccacc gcagaattac aaaattctgc tgcagtaccg ccagacaatc
     6601 tcccctcaag cactccagcc acaaaatccc gaaactccct tccggaaggg aagggtttgg
     6661 gatgggttag atcagtattt atatcttcct cttcctcttc ctctggtaac gctttttgat
     6721 ccgtttgtgt aacgctgcca gcgttacctt ttcgtttcag ttcgcgtatt tttgttactc
     6781 gctcgtttgt aaccgcccgt tttttagagc ttttcccgtt atgacgttca aagttaggta
     6841 gagaaagccc accgtcattt tcgaccagcc atccaacctg aattaacgca tcagcaaaac
     6901 cagccataaa agtgatgcga tctattgcac tttttgtaac gccgcgagcg ttacaatctg
     6961 cattaccgtc tatcatttgt tgatccgccc atgcccagaa gcgaataacc ttccctaatg
     7021 cggcatctgg atcaatattc agaatctcag caagcctgaa tatttccggc ttatccggcg
     7081 taataacctc gagctttatc cagtttgaag ccatttgttt tcaccttgta acgctcgcag
     7141 cgttacattt aactgatacc gaacaaaaca atccggcacg attaatttca atcaatgcac
     7201 tacgacagaa tcgccgggcg acccaccacc gctgaaatgt gctttccggt aaacggcctg
     7261 gactgcatca tcatgcgcat caattgccgt actcaacgct tcctgcgccg ccagtaatgc
     7321 acggcgttcc agggtatcga agatgcagag tcggtgacgc agctcgcgcg gaagaattgc
     7381 cagaaccgca gggatcagtt tctgaatttt ttccctttgc gctttcgttt cacctttcaa
     7441 ccaacggtga tagatattct gctgattatt ccagtccttg cctggtacca ggggcaattc
     7501 gccgcccccc tggcgcagat attcttcagt aattgcgtta gcgacccacg cctgcccttt
     7561 ttcggctgcc agggctaaca acactgattc gatgtgctca tgcctgattt tcatgaatca
     7621 accgctccta tgctgttttc gctatgctta ccgtctgggg ggaatacatc gtcaagtcca
     7681 caatgagcgc caagccgatt aagggtagaa acaatttttc tgcactcctc tagtccaggg
     7741 gtacgaaaat ttgcttcgta atttgccagt cggctttgta tccaccctaa ctgaacagca
     7801 agttgtcttt gagacagccc aagctgtttt cgatatgttg aaattttgtt catcattacc
     7861 tcctgtttct attattcaca tttcgtgagt gagatcaact acagatacgt gagtaccgcc
     7921 aatttcacac atcgtgataa aatcggaact atgaaaacga ttgcagaaca aataggcgag
     7981 cgcctaaaag ccactcgcct aaatcgagga ttaagtatgg gaaacctggc aaaactatgt
     8041 ggttggtcag gttcttctcg tattgctaac tacgaagctg gcaccaggag tgtgggggct
     8101 gaggatgcga tcatcctcgg ccaagtacta ggcatttccc ccgcagaatt aatgttcggt
     8161 gaacaggaaa acacgtactc atggttaaat gataaccaga aaaaaatgct agagctgttt
     8221 aatcagctcc cgggatcaga acaagcacgc atgattgatc tgttcgaaat ccgtcttcaa
     8281 gagcttgatg agtatgtgga aaaatatcta agaagcaggc aacagaaaaa atcctcctaa
     8341 acctttaatc tccctcgatt ccaatcaaaa agctctcgct attagcgagg gctttttttg
     8401 tccatcaaaa atccaaaaac aacattgttc acatattgag ttgacaaatc actcacgata
     8461 tgtgagcatt cgtcatatcc aaacaacgcc ccaccagaga acggcaggac aatacctcga
     8521 gttatccagc cactgaacag ggctaagtag ccagcctgag gcatacgaac atgacggcag
     8581 ttgttgattg atacaaagcg cagtagataa aacgttccgc cacccggcgt taaggggaaa
     8641 tgaggtcaac atggatacta tctatcttgg caacaacgaa tctctggtat gtggcgtgtt
     8701 tcccaatcag gacggcacat tcaccgccat gacgtatacc aaaagcaaaa cgtttaaaac
     8761 cgaagctggc gcacgtcgtt ggttgggaag acattcaggt gagtaaaatg aacgagacag
     8821 aattaaaaca cgttatcgct ctactcctgg aagatgcaaa acgcctccag caactggagc
     8881 caaatgcagg cactggggca cgcatctggc tggctaaaga agcactggaa tctggcgatt
     8941 atgatagcga agaagccttc tacaaagcag aaggccgtgc aggatattca ccgggtcttg
     9001 gcggggtata aataccatgc gcattgactg aattcgcaaa caaaaacaga cgcgcgatac
     9061 ctggatagtc ggtctgcata gtcagatatc tggctgcgaa tttgataaat atcaccgccc
     9121 ttttcggaaa cataagttcc gtccgggagt tgattatacg aaccatctcc atgggggatc
     9181 gttctcctga atcctagtga gagcatatat ttatgaagcc cttcgtaatc ctctggctca
     9241 gcattatata gttctactct ggcgagatac gttggcatat tcatttcctt actggttgtg
     9301 tgagaacttc agtaaagata ccaccaaagc ccggaggtgg tgaaataaaa ccgggcacaa
     9361 cacgaaggcg catttccggt attcataaag agtcggtctt gtctgttaaa tttaaatggt
     9421 gggagtgcgc ctccggttgt aaataacgac attgctgtgt gtagtcctgg cggcatcagt
     9481 ttttttcttg aagttcggct gatgtccgcc ctttttaaag tgaattttgt gatgcggtga
     9541 atgcggctaa gcgcacgtgg cacagttaaa agtcatgtta gtccttattg gtttgggtgg
     9601 gaaagccgac tgtaattgtt aactggttgc agtcacctgg aggcaccagg caccgcatca
     9661 acaaagttca tttgtaaaaa tggagataat tatgattgca catcacttcg gaactgatga
     9721 aataccacgt cagtgtgtga cccctggcga ttatgttctt catgaaggtc ggacatatat
     9781 cgcctcggca aacaatatta aaaagcgaaa actttatatt cgtagcctga ctacaaaaac
     9841 atgcatttct gactgcatga ttaaagtctt cctcggtcgt gatggtttac ctgtaaaggc
     9901 ggagtcatgg taatgactaa gaaaataaaa tgtgcttatc acctttgcaa taaagaaatt
     9961 gaagaaagca aaatcattac aagaccactt catttcatgc gtggagttat accaacgacg
    10021 gaaatgaaaa aatattgtag tgaaatctgt gccgaaaaag accagatggc acacgaactt
    10081 taattaactg actatccgaa actgaattta tgccagcaat ggcagggatt cgctcaacct
    10141 taattaagga gaaaaacatg attaccagtt atgaagccac tgttgttact actgatgaca
    10201 ttgttcacga agtcagcctg gaaggaaagc gtattggcta cgtgattaag acagaaaata
    10261 aagaaacccc attcactgtg gttgatatcg acggtccatc aggcaacgtt aaaacactta
    10321 acgatggtgt taaaaaaatg tgtctggtgc acataggaaa gaatctgccc gcagaaaaaa
    10381 aaagccgaat ttctggcaac tctgattgca atgaaattaa aaggtgaaat ctgaaaaaaa
    10441 gaaagcctgc acactgtgca ggcctgagtg aagaacctgg gacatttatt catcactcgc
    10501 agtaatttta atctgagttg aggttaaaaa acaatgagca ccgataaaca agtttaccca
    10561 ctgtattacg aagcaaaaaa tgacaaagta agaaaacgtc tcggtattaa aggcggtttc
    10621 tactgggctg aagcgaaaaa attatccatt gccatctccc gtggtgctgt tgcgattgac
    10681 gatgctggct acgatgaaga tgactttaaa aaacctgttc gcgtcaattt gcccgttgtt
    10741 gatgaccttc caccagaagg cgtatttgat acggaattct gcaaccgtta cgaaaaaggc
    10801 ggggaagatg gcatcacaat ggtatttatc gcgccctcat cctctgcgca ggacaaacca
    10861 gccagcactg acaataccaa tgttaatggc gaagacatga ctgagattga ggagaatatg
    10921 ctactcccga tttctggcca agagctgccc attcgctggc ttgctcaaca cggcagcgaa
    10981 aaaccggtaa cgcacgtttc acgcgacgaa ctccagacat tacatattgc acgggctgaa
    11041 gaactaccag ctgttactgc cctggctgtt tcccacaaaa ccagcctgct cgacccgctg
    11101 gagattcgcg atcttcacag actggttcgt gatactgaca aagttttccc taatccaggc
    11161 aattcaagtc tggggctgat gactgctttt ttcgaagcat acctggacgc agactacacc
    11221 gatcgcggtc tgctgacaaa agagtggatg aaaggaaatc gtgtttcacg catcactcgc
    11281 acggcttccg gtgctaatgc tggcggcggg aacctcaccg atcgcggcga aggtttcgtc
    11341 cacgatctga cgtcactggc acgcgatgta gccactggcg tactggcccg ttcaatggac
    11401 gtggacattt ataaccttca tccggcacac gctaaacgtg tcgaggaaat tatcgctgaa
    11461 aataaaccgc ccttttctgt tttccgcgac aaattcatcg ccatgcctgg cgggctggat
    11521 tattcccgcg ccatcgtggt tgcgtccgta aaagaagcac caattgggat cgaggttatc
    11581 cccgcacacg tcactgaata tctgaacaaa gtactgactg aaaccgatca tgccaaccct
    11641 gatccggaaa tcgtggatat tgcctgcggt cgctcctctg ccccgatgcc gcagcgggta
    11701 acagaagaag aaaaacagga tgatgaagaa aaaccgcaac catcttgcgc aatggcagat
    11761 gaacaggcaa cggctgaaac agtggaaccg gatgcaactg aacatcatca ggacacgcag
    11821 ccgctggatg ctcagtcaca ggtaaattct gttgatgcga aatatcagaa actgcgggca
    11881 gaactccatg aagcccggaa aaccattccg cccaaaaatc ctgtcgatgc cgacaaattg
    11941 ctggctgcct ctcgcggaga atttgttgag gggattagcg acccgaatga tccgaaatgg
    12001 gtgaagggga ttgaaacccg cgattcagtg aaccagaacc aacaagaatc ggaacagaac
    12061 gaccagaaag cggaacaaaa cagcccaaat gcgttacaaa acgagccaga aacgaaacag
    12121 cctgaaccag tggcgcaaca ggaagtggaa aaagtttgca atgcctgcgg tcagtctggc
    12181 ggggataact gccctgactg tggtgcggtg atgggcgacg caacatatca ggaaacattc
    12241 gatgacgaga atcaggttga agttcgggaa aatgagccgg agaaaatgga aggcgctgaa
    12301 catccgcaca atgagaatgc tgacagcgat ccgcatcgca attgcagtga tgaaactggc
    12361 gaagcgtcag ctcctgtagc aactgaaatc atgtggccgt catatttcga gccaggccgc
    12421 tatgaaaacc tcccgaacga ggtttatcac tccgccaacg gaataagcag cacgatgctg
    12481 aaggatgccc gtatcagcct gatgtattac cacgggcggc acattgccgg aactattccg
    12541 aacgaggaaa gtgatgcact gctgcgtggg cggatcattc acagctatgt tctggaaacg
    12601 gataaattcg ctgatgaata tgccattccg gtaccggttc ctgaatatgt ggttactact
    12661 tctaacgaac tgatcgccat cattaaaaaa cacaatgcca gtctgccagc actgatgaca
    12721 ccagagcaga tgaaagagtg gatcgaaagc tacaacagca ctcttataca gccactgtct
    12781 gtaagtgctg gggccgaaga aacaggcatc ctttacggtt cgcttccggt ggaatttcgg
    12841 cgtattcctg agggggaaaa acacacagca tcagcaatga aagcctgtat taaagaatac
    12901 aacgcaagcc tccctcctct gttgaaaacc agtggagcac gggagcagct tctggatcaa
    12961 attgaaactg tagatccaga actggcaaaa aaagaacgtg ctaaatcttt gccttacaac
    13021 atcagtggca caaaagagca attaaccgaa atcgcacgga aaattcgccc ggaactggtg
    13081 acactggagg actggcaaaa acgccagcaa gaagaaaacg ccgggaaaac gtttatcagt
    13141 ccggatatgt atgaacaggc aaaaaatatt cacgcggcac tgcaaaacaa taccgatgca
    13201 gcaaggctac tcaaccaccc ggatcgcaaa tctgaaatca gctatttcgg gtttgatgaa
    13261 gaaaccgggc tggaaatcag ggtccgtcct gatatcgaaa tccggctgcc atacgaaagc
    13321 atttgcgctg acgtgaagtc agtcagcctc ggttatgtgc ggcaggaacg acttaaagat
    13381 cgcctgcacc gtgaaattat tgagcgtgat tatcacctca gcgccgcaat gtattgcgat
    13441 gtggcaaacc tggacaaatt tttctggatc ttcgtcaaca aagatgctgg ctatcactgg
    13501 gtggcagtcg tggaagcctc gcaggaactc cttgaacttg gtcgacagga atatcgccgg
    13561 acgctacacc agataaacga agcgctggag acaaacaact ggccagcacc gattaccgaa
    13621 agttataccg acgaattaaa cgactttgat cttcgtcgtc ttgaagcact gagcatctga
    13681 ggaaggacac aatgaacgaa ttaactcaac aagaaaatat taactctaat gttgcggttt
    13741 tcagccctca gtctctggct gcaattcaga cgttttctca ggtaatggct tccggcatgg
    13801 ctactgtacc ggaacacctc cggggaaatc catcagactg catggccatc accatgcagg
    13861 cgatgcagtg gcaaatgaac ccttacgcag tagctcagaa aactttcgtt gtgaatggtg
    13921 tactcggata tgaagcgcaa ctggttaatg ccgtaatcag tactcgtggg ccgttaaccg
    13981 ggcgtattga atatgactgg ttcgggccgt gggaaaaaat tatcgggaaa tttgaaatca
    14041 ggaagaacga caaagggaaa gaatatcgtg tacctggctg gaagctggtc gatgaaaacg
    14101 ggatcggagt tcgcgtccag gcaacactac gcggagagag caaaccacgc gtactggagt
    14161 tacttctggc gcaggccaga acacgtaact caacgttatg ggccgatgat cctcgtcagc
    14221 agcttgccta tctggcactg aaacgctggg cgcgccttta ttgccctgaa gtgattcttg
    14281 gagtgtacac ccgggacgaa ctggacgaac cacaggaaaa aatcattaat ccggttcagg
    14341 aacataaaaa cacatccgcc tgccgtgcgg aacgtgaaac aacaattatt gagcaggatg
    14401 tcggggaaaa ctggatcagt gctttccgtg aacgtattga gcaggcacaa agcaccggag
    14461 aaacaacagc acttcggcag gaagtggaag agcataaaaa tacacttggc gctctctata
    14521 cggaacttaa aggaaaagtg gttcagcgtc atcaccgtct caatgctatt gcccgtattg
    14581 agaagatgat aaatgacctg ccttcatcag gtgatccgga agcagaacaa aaatttactg
    14641 ctctggaaaa tacgctgaat gctgcacgac cacatctggg agaattatat gaggcgtata
    14701 aaacgacact gacagatatg aaaccagaat atatcggctc ctgatattta ctatggcggt
    14761 gtagcctcgc cgccataaca aaactttatt ttatgagaga aaagacaatg cggtatgaaa
    14821 aagtcaaacc atgtccattt tgtggttgtc catcagtaac ggtgaaagcc atttcaggat
    14881 attaccgagc gaagtgtaac gggtgcgaat cccgaaccgg ctatagtgga agtgaaaaag
    14941 aagcacttgc aagatggaat aaacgaacta ctggaaataa taatggaggt gttcatgtat
    15001 aaaattaccg ccactattga aaaggaaggt ggcacaccta ctaactggac aagatattca
    15061 aaatctaaat taacgaaatc agaatgcgaa aaaatgctct cagggaaaaa agaagcaggc
    15121 gtttccagag agcagaaagt aaaactgata aattttaatt gcgagaaact tctgtcctcg
    15181 tgagctgcat tatatacaaa ttagaacttc atagctgatt attaaaaatc aaccacaccc
    15241 gccagtattc tgtatattta ctggcggtca tatcgtaaga ggtatggcaa tgaatcttgt
    15301 gacactcaaa acgtggggaa aactcagata tccggataac ccaccatcaa tatcaacgct
    15361 gagacgatgg gcaaggaatg gaaacattta tcctgcacct gaactacacg ggaggagtta
    15421 cagggtggtt ccggaggctt tctatatcaa cccaaataag gttgataccg atataacaca
    15481 ccatcagcct aatgggcgac aagggagaga cagtccgtta ctggagaagt taaaacatgc
    15541 agcggaaaaa atacgatccc aatttgccta ggaacttaac atatcgaagg agggacaaag
    15601 catattactg gcgcaaccct ctgacgaaag aagaatttac actaggtaaa atttcaagaa
    15661 gagatgcaat cgcacaggca attgaagcaa atcattatat atacaaaaac tactctcctg
    15721 ctgccttaat tgaaaaactt aaagggttcg actcatttac tatggcagac tggattgaac
    15781 gttacaaaac gattcttata aggagaaaag tgtccagaaa tacttataaa attcggggaa
    15841 atcaactgga aacaataaaa gaaaaattag gagggatctt actgacagaa ataaccactc
    15901 gccatattgc cgagtttctt gatttgtgga ttgaaggagg gaaaaacacg atggcaggat
    15961 caatgcgttc tgtgttatct gatatgttcc gcgaagccat tgttgaaggg cgtatatctc
    16021 aaaatccagt aacgccaaca agagcaccga aaatagtagt tacaagagaa cggctgaaac
    16081 taaagacata caactgcatc agggaggcag cagatcaact tccggcatgg ttcccattag
    16141 ctatggactt agcccttgta acaggacaac gtcgcgaaga cataacgaat atgcggttca
    16201 gtgatattta tgatgatcgt ctccacatca ggcaaattaa gacaggaatg atgattgcta
    16261 tccccctgtc actcagcctt ccggtcgctg gtttacggct tggtgcagta gttgaacggt
    16321 gccgcatggt aagtcggggg gattatctaa tcagtgccgg aattagaaaa aacagccctg
    16381 acggcagcat tcacccggac ggcctgacaa aaaaatttgt cgcagctaga aaattaacag
    16441 gtatccagtt cagtgaaaac ccaccaactt ttcacgagat cagaagcctg gctggacgat
    16501 tgtacaaaga aacatgtggg gaagaatttg ctcaacgtct acttggccac acatcggaga
    16561 agacaacaaa aatgtatctt gatgagagag aaaaaacgta cttactgctc tgattttaac
    16621 gtaaatggat tgttaaatgt attttggttg tgatataacc aaaaaagacc ggaatacaga
    16681 aattcgagta aatttcgggg aatttcgggg agacgtttgc aaccaattga ttttaaatac
    16741 aattaaaaaa agaccgaata cgattcctgt attcggtcca gggaaatggc tcttgggaga
    16801 gagccgtgcg ctaaaagttg gcattaatgc aggcttagtt gccttgcact ttaagaatag
    16861 atgacgacgc caggttttcc agtttgcgtg caaaatggtc aataaaaagc gcggtggtca
    16921 tcagcttaaa tgttaaaaac cgcccgttct ggtgaaagaa ctgaggcggt ttttttattg
    16981 gaaatcaaaa ggctatttta ggtaattaac agagtttttc agctcgttct ataaacggtg
    17041 ccagactcat tttttcgccg ggattgttag gatcatcaat ctgaatcacc gaaatgggtt
    17101 gggctttagt cttcccactg gcaacttcct tttgtgcgat atcgtttaaa ggatactgca
    17161 cgagggtact tgggttgatg acatacaaag cattacccgg tcggcaagtc agcatcacct
    17221 cttcgcgatt aaacgcccat ttgtctttac ccacttcaaa acggctgacg gtaatcacct
    17281 gcggtgccgc cagcgccgct gcagaactgg tgagtaacag aaacgccaga atactttttt
    17341 tcatcatttt cgtaatccat caaaaaggtt cgagagtcgc aagcaggctg atgatcacca
    17401 gcactaccgc acctatcgcc cattcgagtt tagtcatcca cacaaaccag ggtgccgcac
    17461 gatcttcatc ctgcctcatg cgcggtacaa gaacataacg attagccagc gcgatgacca
    17521 ccataatcat caccaggata gctttgagca ataagagttg gccccagtaa gtcgtaagtg
    17581 tggggggaaa tccggtaatc aacaatgcat taagcacgcc gcttgccagt acgccgatca
    17641 ccgcaaaatg cccgcaccag gaaaaacgca tcagcgcctg aatagcctgg tgtcgccaac
    17701 ggcctttgat gagttgcata caccagagca ccggcagtaa tcccccaaac cacgccgccg
    17761 cacaaatcag gtgaatcgcg tgattagtct gatggatttt cgccgttacc ccttcattca
    17821 gcgtcgcatg ccccactccc gccagcagaa taaattgcgc ggtagtgagc ataaacagca
    17881 aacgtggcat attccgcggt tgcataaggg cgacgatcaa cgttacgagg gcgagaacaa
    17941 tttgccataa ccagacgcca ccaaactgcg tttgcaaaac agcctgccaa atgtttggcg
    18001 aaaatacatc tgtccatccc gttcccatca acccgccctg aacggcgagc attgcagtgg
    18061 cactgatcaa actccagacg gcagcgtgct gttgtaaacg cagaaaacgc ttcgccagca
    18121 agcgacgaat cgttaagggg gccagccagg caccgtacat tgcgaagcca aaaaccagca
    18181 tcagcgaggt gaaatggata aatcgcagcg cgatccaggt aaacgccagc atgatttatt
    18241 tcacgctaaa ggtgtagtgt cctttcgttt tatgcccgtc caccgaaaca acatgccagt
    18301 caacggtgta ggttccgggt ttcagcgaat cggccagtgg aacaatcagt tgcttttggt
    18361 cctgctcatt tcgcttcgcc ggtaatgttt taatattttc gttttttggc cccgtgattt
    18421 ttgcaccgct gaatcctgtt tcaacacctt ccgagaagtt taaagtgatt gcctgcgggg
    18481 cagctgtcac ttgcgcgttt gccgcaggat actgatgcgt taaatgcgca tgtgcccaga
    18541 cggaaggtgt caccaacgaa gtggtcagga ttgccagcgc gtagcgaagg gagcgtgcag
    18601 ttgaaaccat attatctatt cctttttgta ataacttttt tacagagcat aaccttgtct
    18661 aatgtctgag tcgaggatca tcaattccgg cttgccatcc aggctcactc ttagtaactt
    18721 ttgcccgcga atgatgagga gattaagaat gctgaagaat ctggctaaac tggatcaaac
    18781 agaaatggat aaagtgaatg tcgatttggc ggcggccggg gtggcattta aagaacgcta
    18841 caatatgccg gtgatcgctg aagcggttga acgtgaacag cctgaacatt tgcgcagctg
    18901 gtttcgcgag cggcttattg cccaccgttt ggcttcggtc aatctgtcac gtttacctta
    18961 cgagcccaaa cttaaataaa acttatacag agttacactt tcttacataa cgcctgctaa
    19021 attatgagta ttttctaaac cgcacccata atttgtagtc attttgaaaa ggaagtcatt
    19081 atgtcgttct ggaaagttgc agcagcgcaa tatgagcctc gtaaaacctc actcaccgaa
    19141 caggtagctc atcatctgga gtttgtgagg gccgccgcca ggcaacaatg tcagctgtta
    19201 gtttttccat cgctttcttt actggggtgt gattattcgc gacgtgccct tcctgcccca
    19261 cccgatctct cactgttaga cccgctttgt tatgccgcaa cgacctggcg aatgaccatc
    19321 atagccggcc ttcccgttga atataacgat cgctttattc gtggaattgc ggtgttcgca
    19381 ccgtggcgaa aaacgccggg gatctaccat cagagtcatg gtgcatgtct gggccgtcgt
    19441 tccagaacca tcacggtggt agatgaacaa ccgcaaggta tggatatgga cccgacctgt
    19501 tcactgttta cgacagggca atgtctgggg gaacccgacc tactggcatc tacccgccgc
    19561 ttacaatttt tttcacatca gtactccatt gccgtgctga tggcaaatgc ccgtggtaac
    19621 agtgcactat gggatgaaca tggtcgtctc atcgttcgcg ccgatcgtgg ttcattattg
    19681 ttagtcggtc agcgttcatc acagggttgg caaggcgata tcattccatt acgctaggct
    19741 ttttcggcct ggagcatgcc atgttgcgca ttatcgatac agaaacctgc ggtttgcagg
    19801 gagggatcgt tgagattgcc tctgttgatg tcattgacgg aaaaatcgtc aaccccatga
    19861 gccacctggt acgccccgat cgtcctatta gtccacaagc gatggcgatt catcgcatca
    19921 ccgaagccat ggtcgccgat aaaccgtgga ttgaagatgt gatcccacac tattacggta
    19981 gtgaatggta tgtcgcgcat aacgccagct ttgaccgccg cgtactgcct gagatgcccg
    20041 gtgagtggat ttgcactatg aaactggccc gtcgtttgtg gcctgggatc aagtacagca
    20101 atatggcgtt atataaaaca cgcaagctca atgtacagac gccgccgggc ctgcatcatc
    20161 accgcgcgtt gtatgactgt tatatcaccg ccgcgttgct tatcgatatt atgaacacct
    20221 ccggctggac ggcagaacag atggccgata tcaccggacg tccgtcgttg atgacgacct
    20281 tcacctttgg caaataccgt ggcaaagcgg tttccgacgt tgccgaacgc gatccgggct
    20341 atctgcgctg gttatttaat aacctggaca gcatgagccc ggagctgcgt ttaacactga
    20401 aacattatct ggaaaatact tagtccgcag gcgtagcggg taatgttccc tgcgccagcg
    20461 cgaccagaaa agcatattcc atcgctacgc cttcgtacga tttaaagcga ccagatttac
    20521 cgccatggcc tgagtccatg tcggtacaga gcaataaaag atggtcatcg gttttcagct
    20581 cgcgcaattt agcgacccat tttgccggtt cccaatattg cacctgagaa tcgtgcaaac
    20641 cggtcgttac cagtaaatgc ggataagcct gtgcggtgac gttgtcatac gggctgtagc
    20701 ttttcatgta ctcgtaatat tgcggatcct gcgggttacc ccactcttta aactcaccag
    20761 tggtaagagg aattgattca tcaagcatcg ttgttacaac atcaacaaac ggtacctggg
    20821 cgataacgcc gtggaataat tccgggcgtt gattaattgc aacgcccatc aacatgcccc
    20881 ccgcactccc gcccatcgca taacaaagcg aaggagagcc atagcccagt tttaacaatg
    20941 catcgcaggc atcaagataa tcattaaacg tatttttctt cttcagaaat tttccgtctt
    21001 cgtaccattg ttgccccagc tcaccaccgc cgcgaacatg gacaatggcg tagacaaagc
    21061 cacgatctaa caaactcaag cggctaaaac tgaaatcggc atcaatactt gcgccgtaag
    21121 aaccatagcc atacaccagc aacgggttgt gtcctttgcg aaaatgtttg cgatggtaga
    21181 ccaacgaaac cggaacttcg acgccatcac gggcgactat ccacaggtgt tcactgcggt
    21241 aattcgccgc ataaaaacca ggaacttccg tttgttttaa tacacgacgc tcaccggtat
    21301 ccatatccag ttcaaacaaa gtgtctggtg tagtcatgga agaataacca taacgcaatc
    21361 gcgcggtttc aggttctgga ttgtaggcaa tccaggtcac ataggccgga tcatcaaagg
    21421 caataccaat gacttcccgg gtcttgcggt taatttggcg caaactggtt aacccgcgct
    21481 gacgctcttc aaccaccagc cagtcggtaa acagcgtaaa cccttccagc atgatgtttt
    21541 cgcgtggcgg aattaactct tcccactgtt gctcatcacg catacgggta cggtataagc
    21601 caaagttttt gccgtggcgg ttggaacgca gataaaaacg atgctggtag tgatcaaggc
    21661 tgtattcgtg atctttgcgg cgcggcagaa aaacaaacgg ctcggcatcg gccatttccg
    21721 cgtccagcag gcgaacttca ctggtggtgg cgctggccaa atgaatgact acatagtgct
    21781 tcgaggtcgt tttatgcagg ctgacgtaat aggtatcgtc tttttcttcg tagatcagtt
    21841 tatcttgcga tgctggcgta ccgatggcgt gacgccagac ctgataaggc agcagcgtca
    21901 ccggatgctt gcgaacatag tagaaaatcc aggagtcatt tgcccagaca aagctgggtt
    21961 caacgttatc cagcagttcc gggtaccagt taccagtttc cagattacga aaacgaatgc
    22021 cgtactggcg tcgggaaaga aaatcttctg ccagcgccat aatggtgtta tcgggcgtaa
    22081 tcgccattcc gcccatcgaa taaaactcac tatgagctgc gcgcttattg gcatcgagca
    22141 atgtttccca ctcatcccac tcttcactga atgccgattg acgctggtag atagcatatt
    22201 cacagcctgg ttcataaata tgccgatagc ggtagccatt tttgatgtag ggcgcagaaa
    22261 cttctcgttg cggaatgcgg tcgatgattt cctttaagat gcgatcctgc aaggcttgtt
    22321 gtgaggccat cacccgatga ccgtaactat tttcttgttg caggtagtcc aggacttctg
    22381 gctgagaacg cgtatcgtcc cgcagccagt agtaattatc gatgcgcgta tcgccatgaa
    22441 gcgtcatggc gtggggaatg cgggcggctt ttggtagcat gttattgttc tttctggttg
    22501 aaacatctta taagggtggc aaaactcacc ggggatgcaa gcgaaacagg gtcgtcattg
    22561 cttagatgat gacaggtaat ggcgcggata tcgaatgtta tgcagacaga gaaaatcagc
    22621 ctgttcaaat ggctgtgcgg ttctggatag cccgaaatag tcaacttcag gctatccaga
    22681 gagcggaatt attccgccaa agtgcgtttt tgctgttcga gatcgcgttc aatgccgtca
    22741 cgaacatcct gggggatttt cagcgcgtca cccagtgcat tcaggtaact gcgttccata
    22801 aaatggtcaa tatcaatagc cgcgcaactc aggaaataga tttctagcgc ctcttcttca
    22861 ttgcggaccc cggtagccag gcgttgtgga tccagcggtt gttcgattgc ctgctcaatg
    22921 agtacacgcc cctgctcttc cacgccggct tcacgcaatt gctggtcgat tgccgcacgt
    22981 tctttggcat caatatgacc atcactttta gcggcaaaga ccagcgcaag gatcaaacgt
    23041 tccgtacgct catccagcgg cgtactttgc gcgccaaact gcggttcgtc ctgatgcgcc
    23101 gcgcgaattt tatctttgta tttattccac agcaccgtac ccgctaccgc tccgccgcca
    23161 accagtaacg cgttggtgcc atatttcgta agaagtttac gtgctgattt atttgcgacc
    23221 agcagcccag ccagtccgcc taatgcgcct ggcactaaca gtttgcccaa tccctgatcc
    23281 gcagacgaag aggtagaaga actgctttgc ccaagcaggg attgcagttg atttaaccag
    23341 ttagccatat ttgctcctca ataaccattt ttagtgcctg tagcatagcg gatgaggatg
    23401 tcagaaaatg tatatcgaga caaaagatgc gcaaataagc ggggcggtag gccgttattc
    23461 aaagaaattc gcgccaggta aggtatttac tgtatatcag gtgataaggg tattttaggt
    23521 gaaagtagtt tgtcgttttg agtggcattg tttgatgctg attgcccgat gcgacgctgt
    23581 tgcgtcttct caggcctacg attgtggtgc gttctggggc ggacaaggca ctcgcgccgc
    23641 atccgccaac ggtcaatcga attatcagaa gccattaacg ccgctgatat tccgccattc
    23701 ccgctttgca gtccacgctg acctggtaat gaatatcggc acttttacca cgcacggtta
    23761 gcggtactga ccatttatca tctttaccct gaatgtcctg caaactgacc caagccacgg
    23821 gatcggcctg accgacaatt ttttgatcat ctgcccagcg cgccacgcga ttttgttgat
    23881 aatcacgttt tacgctcgcg gcaattccgg cagcatccag accttcacac tttgggaaag
    23941 tgaccgactt gctggtttca ttattggcag cgaaaactga tgcgcaggca gaaaccaaca
    24001 acagccctaa aaacgcccct ctttttttca tgtttttctc catagcacaa tgattcagga
    24061 gaaagcatgg tacaaattgt caggagcgca agttgcttca ggccgcgtgt gaggcatctt
    24121 cctcttcgga ttcagcaccg acgacctgtg gggacggtaa tttgccggtt ttcagaatgg
    24181 tgctcagcac atctttttgt tctgccagcc atagcgaaag ggcttcacgt tgctcgtctt
    24241 ccatttgcac tggagaattt gtcagccagg tgtcaagcag atccgccgta tcaagcattt
    24301 tatcataagc atcggcttcc tttttgctgg taaacgacat tttctcttcg ccctcacgaa
    24361 tgactacgta tttaacttca accgccattt gcagcctctc ataataactg tgattttata
    24421 cagtatattt cttttcggtt gagaaatcaa catcagcaat aaagacacgc gcaaacgttt
    24481 tcgtttatac tgcgcgcgga attaatcagg ggatattcgt tatgacgtta ttaggcactg
    24541 cgctgcgtcc ggcagcaact cgcgtgatgt tattaggctc cggtgaactg ggtaaagaag
    24601 tggcaatcga gtgtcagcgt ctcggcgtag aggtgattgc cgtcgatcgc tatgccgacg
    24661 caccagccat gcatgtcgcg catcgctccc atatcattaa tatgcttgat ggtgatgcat
    24721 tacgccgtgt ggttgaactg gaaaaaccac attatatcgt gccggagatc gaagctattg
    24781 ccaccgatat gctgatccaa cttgaagagg aaggactgaa tgttgtcccc tgcgctcgcg
    24841 caacgaaatt aacgatgaat cgcgagggta tccgtcgcct ggcggcagaa gagctgcagc
    24901 tgcccacttc cacttatcgt tttgccgata gcgaaagcct tttccgcgag gcggttgccg
    24961 ccattggcta tccctgcatt gtaaaaccgg tgatgagctc ttccggcaag gggcagacgt
    25021 ttattcgctc tgcagagcaa cttgctcagg catgggaata cgctcagcaa ggcggtcgcg
    25081 ccggagcggg ccgcgtaatt gttgaaggcg tcgttaagtt tgacttcgaa attaccctgc
    25141 taaccgtcag cgcggtggat ggcgtccatt tctgtgcacc agtaggtcat cgccaggaag
    25201 atggcgacta ccgtgaatcc tggcaaccac agcaaatgag cccgcttgcc cttgaacgtg
    25261 cgcaggagat tgcccgtaaa gtggtgctgg cactgggcgg ttatggtctg tttggtgtcg
    25321 agctgtttgt ctgtggtgat gaggtgattt tcagtgaggt ctcccctcgt ccacatgata
    25381 ccgggatggt gacgttaatt tctcaagatc tctcagagtt tgccctgcat gtacgtgcct
    25441 tcctcggact tcctgttggc gggatccgtc agtatggtcc tgcagcttct gccgttattc
    25501 tgccacaact gaccagtcag aatgtcacgt ttgataatgt gcagaatgcc gtaggcgcag
    25561 atttgcagat tcgtttattt ggtaagccgg aaattgatgg cagccgtcgt ctgggtgttg
    25621 cgctggcgac cgctgagagt gttgttgacg ccattgaacg cgcgaagcac gccgccggac
    25681 aggtaaaagt acagggttaa acccggcaaa aaaacgctac aaaaatgccc gatcctcgat
    25741 cgggcatttt gacttttaca gcttagcgcc ttctacagct tcacgcgcca gcttagtaat
    25801 gcggtcgtaa tcgcccgctt ccagcgcatc tgccggaacc agccaggaac caccgatgca
    25861 cagcacgctt ttcagcgcca ggtagtcacg gtagttagcc ggagaaatac cacccgtcgg
    25921 gcagaaacgg acctgggaga acggacccgc gatcgcctgc agggctttca cgccgccgtt
    25981 agcttcagcc gggaagaatt tgaactcttt caaaccgtag tccataccca gcatcagttc
    26041 ggaaacagtg ctgatccccg gaatcagagg aatagtccct tcggtagcag ctttcagcag
    26101 cggctcggtc agacccgggc taattgcgaa ctgtgcaccc acttcagtga cttctgccag
    26161 ctgctgtgga ttcagcaccg taccggcacc cacaatcgct tcaggcactt ctttggcgat
    26221 agcacggata gcatcaactg cacactcggt acgcagagtc acttccagaa cgcgcacccc
    26281 accagcaacc aacgcttttg ccatcggcac tgcgtgttcc agttttttta ccacgataac
    26341 cggtacaacc gggccggtgg tcaggattga ttctgcactt gttttccagt ttttcatcag
    26401 agttttctct cgcctgatta caaatttgtc gtcttaaaaa gtgatacagg ttgcgccctg
    26461 ttcggcaccg gacagttttt cacgcaaggc gctgaataat tcacgtcctg tccccacgcg
    26521 tgacgcgctc aggtcaggaa tgtgcggttc gcgagcagcc agttccgctt cgtctaccag
    26581 cagcgtcagt tcgcctgtct gtccattcac acgaatgatg tccccgtcgc gcacttttgc
    26641 cagcagcccg ccatcgtagg cttctggtgt tacgtggata gctgacggca ctttacctga
    26701 agcgccggag agtcgtccat cggtaactaa cgaaattttg aaacaccggt ccaataatac
    26761 accaagtggc ggcatgagtt tatgtaattc tggcattccg ttcgcttttg gcccctgatg
    26821 acggacaaca acgacacaat cgcggtccag caaacccact tcaaaggccg gcataacgtc
    26881 atgctggctt tcaaaaacaa ccgctggcgc ttcaatcacc tggttctcaa ccggcacggc
    26941 agaggttttc ataaccgcac ggcccaggtt accgcttaac acttttgtcc caccatgatg
    27001 agagaaaggt tgttcgaagg aagcgatcac attgctgtcg agtgattttt ccgccccttc
    27061 ccgccagtcc agttcaccat tattcagcca tggttcaagg gtataacgag acagaccaaa
    27121 gcctgccacc gtattgacat cttcatgcag caggcctgct ttgagcagtt cacgcaccag
    27181 aaccggtacg ccacctgccg cctggaagtg gttaatatcg gccggaccgt tcgggtagag
    27241 acgtgccatc agcggtacaa catcagaaag gtcagagaag tcatcccagt taatctgaat
    27301 accggccgcg cgcgccatcg ccaccaggtg catggtgtgg ttagtggaac caccggtcgc
    27361 cagcagtgca acgataccgt tcaccaccac tttctcatcg atcatcttac cgatcggcat
    27421 ccattcatta ccattaccgg tcatgcgtgt aacctgacgc gcagcggcag cggtcaaagc
    27481 atcgcgcagc ggagaatccg gatgaacaaa agaagagcct ggcaactgca tccccataaa
    27541 ctccaccacc atctggttgg tgttggcagt accgtagaaa gtacatgttc ccggcgcatg
    27601 gtaagacgcg gcttctgact ccagtaaggc catgcggtcc actttacctt cggcataaag
    27661 ctggcgaata cgcacttttt ctttatttgg caaaccgctt gccatcggtc cagacggcac
    27721 aaacaccgca ggcaaatgac caaacgacag ggctgccatc gtcagacccg ggacaatctt
    27781 gtcgcacaca ccgaggaaca gagcaccatc aaacatgtta tgggacagcc ccaccgccgc
    27841 agacatcgct atcacttcgc ggcttagcag cgacaattcc attccatcct gcccctgggt
    27901 gacaccatca cacatcgccg gaacaccgcc cgcaacctga ccaaccgcat tcgcttcatg
    27961 cagggcttta cgaatgattt ctggatagtg ttcataaggc tggtgcgcgg agagcatgtc
    28021 gttataggag gtgatgatgg cgatattgtt acgcaacatg cttttcaaag aggctttgtc
    28081 ttctggctgg caggcagcga aaccgtgtgc caggttaccg catgccaact gcgaacgatg
    28141 aacggtcgac gttttcgctt gttctatccg ggcgagataa gcagagcgag tctcgcgcga
    28201 acgttcaatg attcgatttg ttacgcgtaa caattgtgga ttcataaagg ctcctgaaat
    28261 tgagttgtca gagcaggatg attcacaacg cgtttcattc agaggattta tgactgaaac
    28321 gcctgtaacc ggagctcata gggcaaaaac gtttcagtca gtgtaataaa aaaagcctcg
    28381 tgggtgaatc cgcacgaggc ctgaaagtgt aaaaattgtt ctacaatctg cgcaagatca
    28441 tgttaccggt aaaataacca taaaggataa gcgcagatat tactcaaact cattccagga
    28501 acgaccatca cgggtaatca tcgccaccga ggcaacgggt ccccaggttc cggcctgata
    28561 cggtttcggc gcatcattgt ccatcgccca cgcctcagta atggagtcta cccatttcca
    28621 ggcttcttcc acttcatcac gacgtacaaa cagtgcctga ataccacgca tggtttccag
    28681 cagcagacgt tcataggcat ccgccagatg cgtctgatta aaggtttctg aatagctcag
    28741 atccagcttg gtgatttgca ggttatgttt gtggtcaagg ccaggaactt tattcagtac
    28801 ctggatatcc acgccttcat caggttgcag acggatagtc agtttattct gcggcagatc
    28861 ctgccacgac tctttaaaca gattcagttc aggtgttttg aaatagacca cgacttcaga
    28921 acatttggtc ggcagacgtt taccagtacg caggtagaat ggcacaccgg cccagcgcca
    28981 gttatcaatg tcgacgcgga tcgccacgaa ggtttctgta ttgctgctct tgttcgcgcc
    29041 ctcttcttcc agatatcccg gcactttttt gccctgggcg aagcccgcag tatattgccc
    29101 acgtacggtt ttttcgcgta cgttggagcg gtcgatgcgg cgcagggact tcagtacttt
    29161 cactttttca tcgcggatgc tgtctgcgct caggtcagat ggcggagaca tcgcaatcat
    29221 gcagagaatt tgcagcaggt ggttttggat catatcgcgc atctgaccgg ctttatcaaa
    29281 atagccccag cgcccttcga tccccacttc ttctgccacg gtaatctcaa catgatcaat
    29341 ggtgcgattg tcccagttat tcacaaacag ggagttagca aaacgcagcg ccaacaggtt
    29401 cagcaccgtt tctttaccaa gatagtggtc gatacggtaa acctggcact cctcgaagta
    29461 ttcgccaacc tgatcattga tttcctgcga ggtcgccagc gacgtcccca gcggtttctc
    29521 catgactacg cgtgccggtt tagcattcag ttttgcctcg ccaagccctt tgcaaattgc
    29581 gccaaaagtg ctgggcggca tggcaaagta gttaatggtg atacgatttt tttgatccag
    29641 catcgcgccg agacggttga atgcagcagt gtcattgaca tcgagattac aaaaatccag
    29701 acgtgcactc agggtgtccc ataaaccttc atcaatggtt tctttcatga aagtttcgag
    29761 cgcctcgcgg acaactttgg tatacgccgc tttatcccag tcagcacgcc ctacgccgat
    29821 aatccgggtg tccgggttga gctgaccggc tttttccagt tgatacaggg aaggcagcaa
    29881 tttacgacgc gcaaggtcgc ctttcgcgcc gaaaatgacc aggtcacagg cctgggctgt
    29941 ttgcgttacc gccatgtcat tctccttaag ttaactaacc cggtacttaa gccagggtat
    30001 acttgtaatt ttcttacggt gcactgtact gcttttacga gcttgcgaaa actgtaaacg
    30061 cttatccacc cgtgcgatta cgggaaaagc gcgcaaagtg cggcaaaact gataaaaaaa
    30121 tcatcgtttt ccattcggta aaaatctgac actgatcatg ttatgaaaaa aaataacaac
    30181 ttttttatct gcttttgtca ttaacacggc acacaggcgt aatatctgac aaaactgcat
    30241 ttcgatttct ttcagtgcgg aaatcgtcat tacccgtgag tctctttaca tcatgaatat
    30301 gctggaaaaa atccagtctc agctggaaca tttgagcaaa tcagagcgca aagttgccga
    30361 ggtcattctg gcttcgcccg ataacgcgat ccattcgagt attgctgcta tggcactgga
    30421 agccaatgtt agcgaaccga cggtgaatcg tttctgtcgc agcatggaca cgcgcggttt
    30481 tcctgatttt aaacttcatc tggcacagag tctggcgaat ggcactccct atgttaatcg
    30541 caacgtcaat gaagatgaca gcgttgaatc atacacaggg aaaatatttg agtccgcaat
    30601 ggcaacgctc gatcatgtcc gtcattcact ggataaatct gccatcaacc gcgccgtcga
    30661 cttgctcact caggcaaaaa aaatcgcctt tttcggatta ggctcttcag ccgccgttgc
    30721 ccacgatgcg atgaataagt tctttcgttt taatgttccg gtggtgtact ccgatgatat
    30781 tgtgctgcaa cgcatgagtt gtatgaattg tagcgacgga gacgtggtgg tgctgatttc
    30841 tcacactgga agaacaaaaa atctggtcga gctggcgcag ctggctcgcg aaaacgacgc
    30901 aatggtgatt gccctcacct ctgcgggtac cccgctcgcc cgggaagcaa cgctggcaat
    30961 taccctcgac gtaccggaag atactgacat ttatatgccc atggtttctc gacttgcaca
    31021 gctgaccgtg atagatgtgc tggcgacagg atttactttg cgacgcggtg caaaattcag
    31081 agataacttg aagcgggtca aggaagcgct gaaggaatcg cgttttgata agcagttact
    31141 taatttaagt gacgatcgct aaaaacgact gtcactgtcc taatcttata cgacatccga
    31201 atgagattaa tttatcgcca tcgcggcgtt atttcattcg gatttcatgt tcaagcaaca
    31261 cctggttgtt tcagtcaacg gagtattaca tgtccagaag gcttcgcaga acaaaaatcg
    31321 ttaccacgtt aggcccagca acagatcgcg ataataatct tgaaaaagtt atcgcggcgg
    31381 gtgccaacgt tgtacgtatg aacttttctc acggctcgcc tgaagatcac aaaatgcgcg
    31441 cggataaagt tcgtgagatt gccgcaaaac tggggcgtca tgtggctatt ctgggtgacc
    31501 tccaggggcc caaaatccgt gtatccacct ttaaagaagg caaagttttc ctcaatattg
    31561 gggataaatt cctgctcgac gccaacctgg gtaaaggtga aggcgacaaa gaaaaagtcg
    31621 gtatcgacta caaaggcctg cctgctgacg tcgtgcctgg tgacatcctg ctgctggacg
    31681 atggtcgcgt ccagttaaaa gtactggaag ttcagggcat gaaagtgttc accgaagtca
    31741 ccgtcggtgg tcccctctcc aacaataaag gtatcaacaa acttggcggc ggtttgtcgg
    31801 ctgaagcgct gaccgaaaaa gacaaagcag acattaagac tgcggcgttg attggcgtag
    31861 attacctggc tgtctccttc ccacgctgtg gcgaagatct gaactatgcc cgtcgcctgg
    31921 cacgcgatgc aggatgtgat gcgaaaattg ttgccaaggt tgaacgtgcg gaagccgttt
    31981 gcagccagga tgcaatggat gacatcatcc tcgcctctga cgtggtaatg gttgcacgtg
    32041 gcgacctcgg tgtggaaatt ggcgacccgg aactggtcgg cattcagaaa gcgttgatcc
    32101 gtcgtgcgcg tcagctaaac cgagcggtaa tcacggcgac ccagatgatg gagtcaatga
    32161 ttactaaccc gatgccgacg cgtgcagaag tcatggacgt agcaaacgcc gttctggatg
    32221 gtactgacgc tgtgatgctg tctgcagaaa ctgccgctgg gcagtatccg tcagaaaccg
    32281 ttgcagccat ggcgcgcgtt tgcctgggtg cggaaaaaat cccgagcatc aacgtttcta
    32341 aacaccgtct ggacgttcag ttcgacaatg tggaagaagc tattgccatg tcagcaatgt
    32401 acgcagctaa ccacctgaaa ggcgttacgg cgatcatcac catgaccgaa tcgggtcgta
    32461 ccgcgctgat gacctcccgt atcagctctg gtctgccaat tttcgccatg tcgcgccatg
    32521 aacgtacgct gaacctgact gctctctatc gtggcgttac gccggtgcac tttgatagcg
    32581 ctaatgacgg cgtagcagct gccagcgaag cggttaatct gctgcgcgat aaaggttact
    32641 tgatgtctgg tgacctggtg attgtcaccc agggcgacgt gatgagtacc gtgggttcta
    32701 ctaataccac gcgtatttta acggtagagt aagtacgttg ccggatgcgg cgaaaacgcc
    32761 acatccggcc tacagttcaa tgatagttca acagatttcg aatattctga agcaaacttg
    32821 aacttatcat caggcgaagg cctctcctcg cgagaggctt ttttatttga tgggataaag
    32881 atctttgcgc ttatacggct ggatttcgcc cggtttgcga gttttcagca attttagtat
    32941 ccaggtgtat tgttctggtc gcggaccaac aaaaatctcg acttcttcat tcatccgccg
    33001 cgcaatcgta tgatcatccg cctctaacag atcatccatc ggtgggcgca cctgaatcgt
    33061 cagacgatgc gtcttgccat cataaatcgg aaacagcggt acaacgcgcg cacggcacac
    33121 tttcatcaaa cgaccaatcg cgggcaacgt cgctttatag gtggcaaaga aatccacaaa
    33181 ttcgctgtgc tctgggccat gatcctgatc gggtaaataa tatccccagt acccctgacg
    33241 taccgactgg atgaatggtt taataccgtc atttctcgca tgcagacgac cgccaaagcg
    33301 acgacgcacc gtgttccaga cataatcaaa aaccgggttg ccctgattat ggaacatcgc
    33361 tgccattttc tgcccttgcg aggccatcag catggcagga atatcgacgg cccaaccgtg
    33421 cggcaccaga aagataactt tctcgttatt acgccgcatc tcttcgatga tctccagccc
    33481 ttgccagtca acgcgcggct gaattttctc cggcccgcgt attgccaact cagccatcat
    33541 tgccatcgct tgcggcgcgg tggcaaacat ctcatcaaca atcgcttcgc gttcagcttc
    33601 actacgttct ggaaagcaga gcgacagatt gattaacgca cgacggcgtg agctttttcc
    33661 cagtcgtccg gcaaaacgtc ccagccgtgc cagaatggga tcacggaact ttggcggcgt
    33721 taaagcgata cccgccatcg ctgctacgcc cagccatgct ccccagtagc gcgggtggcg
    33781 aaaggattta tcaaactcag gaatgtattc gctattattt tttttcgttt ccatgctttt
    33841 ccagtttcgg ataaggcaaa aatcaatctg gtgatagtgt agcggcgcaa cttgccccgc
    33901 accaaataaa aaaagccggt actgactgcg taccggctgc gaatggatgt taattaatca
    33961 aaccgtagct gcggcacaat ctctttggcc tgcgccagga attcgcgacg atcggagccg
    34021 gtcagccctt cggtacgcgg cagttttgcc gtcagcgggt ttacggcctg ctggtttatc
    34081 catacttcat agtgcagatg cggcccggtt gaacgtccgg tattaccgga aagcgcgata
    34141 cggtcgccac gtttcacctt ctgtcccggt ttcaccagaa tcttgcgcaa gtgcatataa
    34201 cgcgtggtgt agctgcgacc atgacgaata gccacataat aacctgctgc gccactgcgt
    34261 ttggcaacca ccacttcacc gtcacccact gaaagcactg gcgtaccctg cggcatggcg
    34321 aaatcaacac ctctgtgtgg tgcaacgcga ccggtcaccg gattagtacg acgcgggtta
    34381 aagttagacg agatacggaa ctgtttcgcc gtcgggaatc gcaagaatcc tttcgccaga
    34441 ccagtaccgt tacgatcgta gaatttgcca tcttcagcgc ggatagcgta ataatcttta
    34501 ccttctgaac gcaaacgtac gcccagcagc tggctttgct cacgtttacc atcaagcatt
    34561 tctcgagaca ttaacaccgc aaattcatcg ccttttttca gtttgcggaa atccatttgc
    34621 cactgcatgg ctttaatcac tgcgctcact tcggcgctgg ttaaaccggc gtttctggcg
    34681 ctggcaacaa agcttccccc gacggtacct ttcagcagat tgttaaccca ctctccttgc
    34741 tgcatttcgc tggtcatttt aaaaccgtta gcggcagtac ggtcataggt tcgggtttca
    34801 cgacgagaca cttcccaggt gaggcgctgc agttcgccgt ccgcggttaa tgtccaggag
    34861 agttgttgac cgattttcag gttacgcaat tctttgtcgg ccgcagccag ttgggtgata
    34921 tcacccatat caataccata ctgattgaga atgctgctta gcgtatcgcc agtggaaaca
    34981 acatattcat gcacgcccgc ttcaccggcg attttgtcat ccagttcgtc ctggggaatg
    35041 gcttcatctt cttgtgcagc ttgatcaatc ggctcactgg cttcaggtaa gagcgaacga
    35101 atttcgttct gttccagctc aatggttttg acaattggcg tggcatcacg gtgataaaca
    35161 taaggccgcc agacagcgac ggccagagta agaacggtga gcgaccccaa cataacgcgg
    35221 tgtggtcgcg gtaaattatt aaacgccagg gcgacagagc gggctatctg ttgcacgtaa
    35281 tcacttcctc attaatctcc tttcaggcag ctcgcatact ggttggctaa ttgactcagg
    35341 aattctgaat agcttgtttt acccagtttg atattcgtcc ccaggggatc caacgttccc
    35401 atacgaacgg atgtccctcg tgcgacgctc tcaacgaccg ctggcctgaa ctgtggctca
    35461 gcaaaaacgc aggttgcttt ttgctcaacc aactgtgttc ttatttcatg taaacgctgc
    35521 gcgccaggtt gaatctcagg gttaacggta aaatgaccaa gcggtgtcag tccaaactgt
    35581 ttttcgaaat agccgtaagc atcgtgaaaa acgaaataac ctttcccctt gagcggcgcg
    35641 agctcgttac caacctgcgt ttcggttgag gctaattgtg cctcaaaatc cttcaggttg
    35701 gcgtcaagtt tggctcgact ttgcggcata agttccacta attttccatg gattgcaacc
    35761 gctgtagccc gcgctatctc tggggaaagc caaagatgca tgttgaaatc gccgtgatgg
    35821 tgatcttcgt cacttttttc cgcgtggtcg tgatcatcat catcgccgtg aatacttttc
    35881 atcagtagcg gtttcacatc ttcaagctgc gcaatcgtta cctgcttcgc ttctggtaat
    35941 ttgcttaccg gtttttgcat aaacgcttcc atctccgggc caacccaaac gactaagtcc
    36001 gcgttctgta agcgttttac atccgatgga cgcagtgaat aatcatgttc agaagcgccg
    36061 tcaggcagta aaacctccgt ttctgttaca ccatcagcaa tggcagaagc gatgaaccca
    36121 acgggtttaa gcgaagcgac aacggcggca tctgcggcct gtgttgcacc tccccagaga
    36181 gcggcggata atgctgcgaa aagaagcgtt tttttatgta acataatgcg accaatcatc
    36241 gtaatgaata tgagaagtgt gatattataa catttcatga ctactgcaag actaaaatta
    36301 acatgacaag tctggtttcc ctggaaaatg tctcggtttc ttttggccaa cgccgcgtcc
    36361 tctctgatgt gtcgctggaa cttaaacctg gaaaaatttt gactttactt gggccaaatg
    36421 gcgcaggtaa gtcgacactg gtacgggtag tgctcgggct ggtaacaccc gatgaagggg
    36481 ttatcaagcg caacggaaaa ctacgcatcg gctatgtacc gcagaagctg tatctcgaca
    36541 ccacgttgcc actgaccgta aaccgttttt tacgcttacg ccctggcaca cataaagaag
    36601 atattttgcc tgcactgaaa cgtgtccagg ccggccacct gattaacgca ccgatgcaaa
    36661 agctctcggg tggcgaaacg cagcgtgtac tgttagcgcg agcattgtta aatcgcccgc
    36721 aattattagt gctggatgaa cccactcaag gcgtggatgt aaatggccag gtggcgttat
    36781 atgaccttat tgaccagttg cgtcgcgaac tggattgtgg cgttttaatg gtttctcacg
    36841 atctgcatct ggtgatggca aaaaccgatg aagtgctgtg cctgaatcac cacatttgtt
    36901 gttccggcac accggaagtt gtttccctgc atccggagtt tatttcaatg tttggtcctc
    36961 gtggtgctga acaactgggt atctatcgcc atcatcataa tcatcgtcac gatttacagg
    37021 gacgaattgt tttgcgtcgg ggaaatgatc gctcatgatt gaattattat ttcccggttg
    37081 gttagccggg atcatgctcg cctgtgccgc gggtccgctg ggttcgtttg tagtctggcg
    37141 tcgtatgtct tatttcggtg atacgctggc tcatgcctca ttacttggcg tcgcgtttgg
    37201 tttgttgctg gacgtgaatc cattctatgc ggtgattgcc gttacgctgc tgctggcggg
    37261 cggtctggta tggctggaga agcgtccaca gctggcgatc gacacgttat tagggattat
    37321 ggcgcacagt gccctgtcgc tgggcctggt ggtcgttagt ctgatgtcta atattcgtgt
    37381 tgatttgatg gcttacttgt tcggtgattt gctggcagtg acgccggaag atctcatctc
    37441 catcgccatt ggcgtggtca tcgtggtggc tattttgttc tggcaatggc gcaatttgct
    37501 gtcgatgacg attagcccgg atctggcgtt tgttgatggt gtgaaattac agcgcgtgaa
    37561 attgttattg atgctggtga cggcattgac gattggtgta gcgatgaaat ttgtcggtgc
    37621 gttgattatt acttcgttgc tgattattcc tgcagctact gcgcgtcgct ttgcccgcac
    37681 gccggaacag atggctggtg tcgctgtttt ggtggggatg gtggcagtga ctggcggttt
    37741 aaccttttcc gcattttacg atacgccggc gggtccgtcg gtggtgctat gtgcggcact
    37801 gttatttatt ctcagtatga tgaaaaagca ggccagctaa tctatcgctg aacacatttg
    37861 tcggatgcgg cgcgagcgcc ttatccgacc tacggttcgc tatctctggc tggcctgata
    37921 agacgcgaac agcgtcgcat caggcacact gccaatgtcg gatgcggtgc gagcgcctta
    37981 tctgacctac ggttcgctat ctctcgtagg ccggataaga cgcgaatagc gtcgcatcag
    38041 gcacactgcc aatttcggat gcggtgcgag cgaccaatcc gacttacggc atttctggcg
    38101 gcgttatgcc aaagtgattc cacgcccgcg tcgtcgccat acgcccacgc ggtgtacgct
    38161 gcaaaaagcc ttgctgaatc aaataaggtt ccagcacatc ctcaatggtt tcacgttctt
    38221 cgccaatggc tgccgccagg ttatccagac ctacaggtcc accaaagaac ttatcgatta
    38281 ccgccagcaa caatttgcgg tccatataat cgaaaccttc agcatcgaca ttcaacatat
    38341 ccagcgcctg agcagcgata tctgccgaga tggtgccatc gtgcttcact tcggcgaaat
    38401 cacgcactcg acgcagcaga cggttggcaa tgcgcggcgt accgcgagcg cgacgagcaa
    38461 cttccagcgc gccgtcatca ctcatctcaa gccccataaa gcgtgcgctg cgactgacga
    38521 tatattgcag atccggcacc tgataaaact ccagacgttg cacaatacca aaacggtcgc
    38581 gcaacggtga tgtcagcgaa cctgcgcgcg tggttgcacc aatcagggta aacggcggca
    38641 aatcaatttt aatggagcgt gccgccggac cttcaccaat catgatatcc agttggtagt
    38701 cttccattgc cgggtacagc acttcttcaa caactggcga tagacggtgg atctcatcaa
    38761 taaacagcac gtcatgcggt tcaaggttag tgagcatcgc agccaaatcg cccgcctttt
    38821 ccagcaccgg accagaagtc gtgcgtaaat taacgcccat ttcattggcg acaatgttgg
    38881 caagcgtagt tttacccaac cccggaggac caaaaatcaa caaatgatcg agggcatcgc
    38941 cgcgcagttt cgctgctttg atgaaaatct ccatctgtga acgaacctgc ggctgaccaa
    39001 catactcttc cagtaatttg gggcgaatgg cgcgatctgc tacatcttcc ggcaaagtgg
    39061 taccggcaga aatcagacgg tctgcttcaa tcatccttta cctcataacg cggcgcgtag
    39121 ggcttcgcga attaatgttt cactgctggc gtcagggcga gcgattttgc tcaccatgcg
    39181 gcttgcttct tgtggtttat agcccagcgc caccagcgcg gcaaccgctt cctgttcagc
    39241 atcgttggtt gccgggctgg caggcgacgt gagcaccaga tcggcggctg gcgtaaagag
    39301 atcgccatgc agacctttaa atcggtcttt catttcaaca atcaagcgtt cggcggtttt
    39361 tttgccaata cccggcagtt tcaccagcgc ccccacttct tcacgctcaa cggcattaac
    39421 gaactgctgc gctgacattc cggagaggat cgccagcgcc aacttcgggc cgacgccgtt
    39481 ggttttgatc aactctttga acaacgtgcg ctcttgttta ttgttaaaac cgtacagcag
    39541 ctgcgcgtct tcacgcacca caaagtgggt gaaaacgatc gcttcctgtc ccgcttcagg
    39601 gagttcataa aaacaggtca tcggcatatg cacttcatag cctacgccgc ccacttcaat
    39661 taacaccagc gggggttgtt tttcaatgat gatgcctctg agtctgccta tcacatgacg
    39721 ctcctgcgta atgaatcaaa gataatgctg tatgataaaa aaatgctgga tagatatcca
    39781 gcgaaggatg aagaaaactt gcgaggtgtc tcgatgatct gaaaaatggc acagtataat
    39841 ttattctaca ggttattttg gaagcaaata ttttaatatt acatattcag tgaaaaaatg
    39901 cgtaataaaa atatacattg cgcctcctga aaaaataaat tttttatgct attacgtata
    39961 ttcgtatcta tttcaatgga atgacaacgt gaatattaat taccctgctg aatatgaaat
    40021 tggagatatc gcctttacat gtataggtgc tgccttattt ggtcaaatat cagctgcatc
    40081 aaattgttgg agtaatcacg tcgggatcat tatcggtcat aacggtgaag acttcctggt
    40141 tgcagaaagc cgggttcccc tctcaaccat cactacgcta tcccgtttta ttaaacgctc
    40201 tgctaatcaa cgctatgcta taaagcgatt agacgctggg ctgacagaac aacaaaaaca
    40261 acgaattgtt gaacaggttc cctcccggct acgcaaactt taccacaccg gttttaaata
    40321 cgaatcttcg cgccagttct gttcaaaatt tgtctttgat atttataaag aggcgctatg
    40381 tattccggtg ggtgaaatag agacgtttgg agaattgtta aatagcaatc cgaatgcaaa
    40441 actcactttc tggaaattct ggttcctggg ttctattccg tgggagcgta aaaccgttac
    40501 gccagccagt ttgtggcatc atccgggttt ggtgttgatt catgcggagg gagtggaaac
    40561 gcctcagccg gaactgaccg aggcggtata acttaacgca gtcgccctct cgccaggttc
    40621 agccgcgatt cgctcatctg catcgcattc tgactaacgt ggcagtgggt gatggcaatc
    40681 gccaacgcat cagcggcatc cgcctgtgga ttagcgggca gtttcagcaa ggtgcggacc
    40741 atatgctgca cctggctttt ttcggcgcta ccaataccca ccaccgtttg cttcacctga
    40801 cgtgcagcat attcaaatac cggcaactcc tgattcaccg ccgccacaat cgccacgccg
    40861 cgcgcctggc ccagtttcag ggctgagtca gcgttctttg ccataaagac ttgttcaatg
    40921 gcgaaataat caggctggaa ctgggtgatg atttccgtca cgcccgcata gatgagtttc
    40981 agacgagacg gtaaatcatc cactttggtg cgtatgcatc cgctacccag gtaggacagt
    41041 tgcctaccta cctggcggat gacgccgtag ccggtcacgc gcgaacccgg atcaatgccg
    41101 agaataatag ccatcacgcg tctccgtttt gctgtttagc aggcctcatc agagagtcgc
    41161 tgcgacctca tcagagattt caccgttatg gtaaacttcc tgcacgtcgt cgcaatcttc
    41221 cagcatatcg atcagacgca tcagtttcgg tgcggtttct gcatccatat cagctttggt
    41281 agacgggatc atggaaactt ccgcgctgtc tgctttcaga cctgccgctt ccagagcgtc
    41341 gcgcacttta cccatttctt cccatgcagt gtagacatca atcgcgccgt catcataggt
    41401 cacaacgtct tcagcacctg cttccagtgc tgcttccatg atggtgtctt catcgccttt
    41461 ctcgaaggag atcacgcctt ttttgctgaa cagataagct acggaaccat cagtaccgag
    41521 gttaccgcca catttgctga atgcatgacg cacttcagca acggtacggt tgcggttgtc
    41581 agacagacat tcaatcatga ttgccgtgcc gccaggaccg taaccttcgt agatgatggt
    41641 ttccatgttt gcatcatcat caccgcccac accacgtgca attgcgcggt tcagtgtgtc
    41701 acgggtcatg ttgttagaca gtgctttatc caccgccgca cgcagacgcg ggttagcgtc
    41761 cggatcgcca ccgcccagct tagccgcggt taccagctca cgaatgattt tagtgaagat
    41821 tttaccgcgc ttagcatcct gcgcagcttt acgatgtctg gtgttggccc atttactatg
    41881 acctgccata aaaatatctc cagatagccc tgcctgttca ggcagcgtta attacaaact
    41941 gttcaatcgc ctgccggttg ctccaggact tagtgagcgc cgccgcagca ggcgcatcaa
    42001 gccacttgta agccagatgt tcagtgaaaa cgatctgccg ctcgtgcgga agcgcaagac
    42061 agaaccatga ttccgtatta cgcgtcacgc ccggcgcata gcgatgacgt aaatgtgaaa
    42121 aaatttcaaa ctctaccgtg cgctgacagt caattaaggt cagttgttca gcgacaacat
    42181 caatggtgac ctcttccttt acttcgcgca tggcagcttg cggcgcggtt tcaccctctt
    42241 ccacgctgcc ggttaccgac tgccagaaat cgggatcgtc acgccgctgc aacatcagca
    42301 cccgtttcgt atcttgtgcg tagatgacca ctaagatcga aacgggacgc ttatacactt
    42361 tatccttcac gctgcctctt cgttgactgc cttcgctcat cccattcaca tagttatcta
    42421 tgctcatggg agttcactca gttgccgcct cgatgcaacg cgaatgattt cgtgtatttg
    42481 agtcatatca gttattctca gccttcttca caacctgaat gctcagctca gccagtgcag
    42541 tcgggttagc aaagctcggt gcttcagtca tcagacacgc tgccgccgtg gttttcggga
    42601 aggcgataac gtcacggata ttgtcggtgc cggtcagcag catggtcaga cggtcaagac
    42661 cgaatgccag acctgcgtgc ggcggagtac cgtatttcag agcgtcgagc aggaagccga
    42721 atttctcgcg ctgttcctct tcgttgatac ccagaatacc aaacaccgtc tgctgcatat
    42781 caccattatg gatacgtact gaaccaccgc ccacttcgta accattgatg accatatcgt
    42841 aagcgttcgc caccgcattt tccggtgcag ctttcagttc tgcagccgtc atatctttcg
    42901 gtgaggtgaa cggatggtgc attgccgtca ggccgccttc accgtcgtct tcaaacatcg
    42961 ggaagtcgat aacccacagc ggtgcccatt tgctttcgtc ggtcagacca aggtctttac
    43021 ccactttcag gcgcagtgca cccatcgcgt cggcaacaat tttcttgttg tcggcaccga
    43081 agaaaatcat atcgccatct tgcgcggcag tacgatccag gatgtcttcg atgatttctg
    43141 cattaaggaa cttcgctacc gggctgttga taccttccag acctttcgcg cgttcgttaa
    43201 ctttgatgta agccagacct ttcgcgccgt agattttaac gaagttaccg tattcgtcga
    43261 tctgcttacg ggtcagcgat gcgccgcccg gaacgcgcag agccgctacg cgacctttcg
    43321 gatcgttcgc cggacctgca aatacagcaa actcaacaga tttcagcaga tcagcaacgt
    43381 cagtcagttc catcgggtta cgcagatccg gtttatcaga accataacgg cgttctgctt
    43441 ccgcaaaggt cattaccggg aaatcgccca gatccacacc cttcacttcc agccacagat
    43501 gacgcaccag cgcttccatc acttcacgca cttgcggcgc ggtcatgaaa gaagtttcca
    43561 catcgatctg agtaaattca ggctgacggt cagcacgcag gtcttcgtca cggaagcatt
    43621 taacgatctg atagtaacgg tcaaaaccgg acatcatcag cagctgtttg aacaactgcg
    43681 gggattgcgg cagtgcgtag aatttacctt tgtgcacacg agaaggcacc aggtagtcac
    43741 gcgcgccttc cggcgtggct ttggtcagca tcggagtttc gatgtcgagg aagccgtggt
    43801 catccataaa acggcgcacc aggctggtga ttttagcgcg ggttttcagg cgctgagcca
    43861 tttccggacg acgcaggtcg aggtagcggt atttcagacg cgcttcttcg gtgttgacgt
    43921 ggttagagtc aagcggcaga acatctgcgc ggttgatgat agtcagcgag gacgccagca
    43981 cttcgatttc gccggtcgcc atatcgcggt taatattttt ttcgtcacgc gcacgtacgg
    44041 tgcccgtgac ctgaatgcag aactcattac gcagttcaga ggccagcttt aacgcgtccg
    44101 cacgatccgg atcgaaaaat acctgcacga taccttcgcg gtcgcgcata tcgatgaaga
    44161 tcaggctacc aagatcacga cgacggttga cccaaccaca cagagtcacc tgctgcccca
    44221 cgtgggacaa acggagctgt ccacaatatt ctgtacgcat gagatatccc ttaacttagc
    44281 tgccggcgga tgccccctgc tgcgcaggtg accaagtcgc agcgttagct gtatgtcaca
    44341 actgaatgaa aaaaggcggc tattatactg gaaattctgc cgcaccgtaa gagcctggcc
    44401 cgcgctggaa cgcctcgtta ccactttata tcgggcctga aatcagactc tacgccagtt
    44461 tgctataaag gtgttgcccg aactcataaa aattaacaaa atttgtcgtt ccgccatcgg
    44521 ctaatcgcat taaggtgtga ggcacgattt tgttttttca ggagtcatta tgcttgaact
    44581 taatgctaaa accactgcgc tggtggtgat cgatttacaa gaaggcatct taccttttgc
    44641 cggtggtcca catactgccg atgaggtagt taatcgcgcc gggaagctgg cggcgaaatt
    44701 tcgcgccagc ggtcagcccg tgtttctggt gcgcgttggc tggtctgccg attacgctga
    44761 agcattaaaa cagccggtcg atgcgccctc accggctaaa gtgttgcccg aaaattggtg
    44821 gcaacatcct gctgcattag gtgcaaccga cagcgatatc gaaatcatca aacgtcaatg
    44881 gagtgcgttt tacggtacgg atctggagtt gcaattacgc cgccgaggta tcgatacaat
    44941 agtgttatgt gggatctcga ccaatatcgg tgttgaatcc accgcccgca atgcctggga
    45001 acttggcttt aatctggtga ttgccgaaga cgcctgtagt gccgccagcg ccgagcagca
    45061 caataacagt atcaaccata tctacccgcg catcgcccgt gtgcgtagcg tggaagagat
    45121 cctcaacgcg ttatgattta catcggtcta ccgcaatggt cgcatcctaa atgggtgcgg
    45181 ttggggatca ccagccttga agagtatgcc cgccacttta actgcgtgga gggcaacacc
    45241 acgctttacg ccctgccgaa acccgaggtt gtcctgcgct ggcgtgagca gaccacagat
    45301 gacttccgct tctgttttaa gtttccggcg accatttcgc atcaggcagc attacggcat
    45361 tgcgatgatt tagtgactga atttttgacc cgcatgtcac cgttggctcc gcgcattggg
    45421 caatactggc tgcaactgcc tgccacattc ggcccacggg agctgcctgc gctttggcat
    45481 tttctcgatt ctcttcctgg cgaatttaat tatggcgtgg aagtccgcca tccacagttt
    45541 ttcgccaaag gagaagagga acaaacgctt aatcgcggtt tacatcagcg cggcgttaat
    45601 cgggtgattt ttattagaca cgtttaaata cactacctaa agcgatacaa tctaggtatc
    45661 agttttgagg tggtaaacaa tgaaacgtgt catttcatac attcgtttct catcagagaa
    45721 gcaagctaaa ggcgactcac tccgcagaca atctaagatg gtcactgatt ggcttaaggc
    45781 tcatcctgag tactaccttg accaagattt atcttttcgt gatttaggcg ttagcgggtt
    45841 tactggtaag cacttgaaag gtggcttagg tgacttctta accgctatcg agaaagggct
    45901 tgttaaagct ggtgatacgc tcttgataga atctcttgac cgtctctcac gtcaggccgt
    45961 agaccgagca agtgagctat taagaaacat cctgcgttct ggtgtagatg tggtcacctt
    46021 gtccgatggt gaacattaca caagggaatc tcttagagac ccgctagctc tgattaaaag
    46081 cattttgata atgcaaaggg cgcatgagga atcattacgt aaggctgagc gtatacaggc
    46141 tgcatgggat gccaagaaag aggctgctaa agctggtatc aaagtttcta aacgttgtcc
    46201 tgcatggctt aagcttaatg atgaccgtaa aagcttctct ataatccctg agaaggtaga
    46261 gacagtaaag agatgctttc agctacggtt agagggcaaa tctatctggg agattgtcag
    46321 gactcttaat gatgaaggtc accagtcgat gagccagtat aagccgggta agtttggtca
    46381 aacctcagtg caagaccttc tgaaaaatcg ctctgtaatt ggttacttca cacctaaagg
    46441 ctgtgaggag atatctggtt attatcctgc aattatagat gaaacaacat tctacaaagt
    46501 ccagcaacta tcatatgctc gttacggtag aaaacctgct tcagataaac cattgagtgt
    46561 gaatctcttt aaaggggtta tccgttgctc tgaatgtggt catgctctga ttatcacagg
    46621 ctttagcgat acaagaagcg ggatttatcg ttgtcctctt cgtaatgaaa accgttgtag
    46681 tgctaaagga ctttcacgta agcaaaccga tggtacatta cttgccctgc tatctcgtct
    46741 tgaccgattt gatagcgata cgacgaatac catagatatg ttgaggctac accgtgaaca
    46801 catctcaagc aatatagaaa agctggtaca actatcactt gaaa
//
LOCUS       DAJOWB010000025        41842 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJOWB010000025 DAJOWB010000000
VERSION     DAJOWB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.025"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            336..1904
                     /gene="cydA"
                     /locus_tag="OHO85_001564"
     CDS             336..1904
                     /gene="cydA"
                     /locus_tag="OHO85_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCQ2670955.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGVSAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1920..3059
                     /gene="cydB"
                     /locus_tag="OHO85_001565"
     CDS             1920..3059
                     /gene="cydB"
                     /locus_tag="OHO85_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCQ2670956.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3074..3187
                     /gene="cydX"
                     /locus_tag="OHO85_001566"
     CDS             3074..3187
                     /gene="cydX"
                     /locus_tag="OHO85_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCQ2670957.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3187..3480
                     /gene="ybgE"
                     /locus_tag="OHO85_001567"
     CDS             3187..3480
                     /gene="ybgE"
                     /locus_tag="OHO85_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCQ2670958.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3630..4034
                     /gene="ybgC"
                     /locus_tag="OHO85_001568"
     CDS             3630..4034
                     /gene="ybgC"
                     /locus_tag="OHO85_001568"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCQ2670959.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4031..4723
                     /gene="tolQ"
                     /locus_tag="OHO85_001569"
     CDS             4031..4723
                     /gene="tolQ"
                     /locus_tag="OHO85_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCQ2670960.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4727..5155
                     /gene="tolR"
                     /locus_tag="OHO85_001570"
     CDS             4727..5155
                     /gene="tolR"
                     /locus_tag="OHO85_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCQ2670961.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5220..6485
                     /gene="tolA"
                     /locus_tag="OHO85_001571"
     CDS             5220..6485
                     /gene="tolA"
                     /locus_tag="OHO85_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCQ2670962.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6618..7910
                     /gene="tolB"
                     /locus_tag="OHO85_001572"
     CDS             6618..7910
                     /gene="tolB"
                     /locus_tag="OHO85_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCQ2670963.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7945..8466
                     /gene="pal"
                     /locus_tag="OHO85_001573"
     CDS             7945..8466
                     /gene="pal"
                     /locus_tag="OHO85_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCQ2670964.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8476..9267
                     /gene="cpoB"
                     /locus_tag="OHO85_001574"
     CDS             8476..9267
                     /gene="cpoB"
                     /locus_tag="OHO85_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCQ2670965.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9432..9507
                     /locus_tag="OHO85_001575"
     tRNA            9432..9507
                     /locus_tag="OHO85_001575"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9465..9467,aa:Lys,seq:ttt)
     gene            9643..9718
                     /locus_tag="OHO85_001576"
     tRNA            9643..9718
                     /locus_tag="OHO85_001576"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9676..9678,aa:Val,seq:tac)
     gene            9721..9796
                     /locus_tag="OHO85_001577"
     tRNA            9721..9796
                     /locus_tag="OHO85_001577"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9754..9756,aa:Lys,seq:ttt)
     gene            9946..10021
                     /locus_tag="OHO85_001578"
     tRNA            9946..10021
                     /locus_tag="OHO85_001578"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9979..9981,aa:Val,seq:tac)
     gene            10025..10100
                     /locus_tag="OHO85_001579"
     tRNA            10025..10100
                     /locus_tag="OHO85_001579"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10058..10060,aa:Lys,seq:ttt)
     gene            10247..10322
                     /locus_tag="OHO85_001580"
     tRNA            10247..10322
                     /locus_tag="OHO85_001580"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10280..10282,aa:Lys,seq:ttt)
     gene            10356..10431
                     /locus_tag="OHO85_001581"
     tRNA            10356..10431
                     /locus_tag="OHO85_001581"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10389..10391,aa:Lys,seq:ttt)
     gene            10462..10587
                     /locus_tag="OHO85_001582"
     ncRNA           10462..10587
                     /ncRNA_class="other"
                     /locus_tag="OHO85_001582"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            10836..11879
                     /gene="nadA"
                     /locus_tag="OHO85_001583"
     CDS             10836..11879
                     /gene="nadA"
                     /locus_tag="OHO85_001583"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCQ2670966.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSISV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            11917..12636
                     /gene="pnuC"
                     /locus_tag="OHO85_001584"
     CDS             11917..12636
                     /gene="pnuC"
                     /locus_tag="OHO85_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCQ2670967.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12633..13574)
                     /gene="zitB"
                     /locus_tag="OHO85_001585"
     CDS             complement(12633..13574)
                     /gene="zitB"
                     /locus_tag="OHO85_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCQ2670968.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13688..14068)
                     /locus_tag="OHO85_001586"
     CDS             complement(13688..14068)
                     /locus_tag="OHO85_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCQ2670969.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14384..15436
                     /gene="aroG"
                     /locus_tag="OHO85_001587"
     CDS             14384..15436
                     /gene="aroG"
                     /locus_tag="OHO85_001587"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCQ2670970.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(15594..16346)
                     /gene="gpmA"
                     /locus_tag="OHO85_001588"
     CDS             complement(15594..16346)
                     /gene="gpmA"
                     /locus_tag="OHO85_001588"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCQ2670971.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(16551..17591)
                     /gene="galM"
                     /locus_tag="OHO85_001589"
     CDS             complement(16551..17591)
                     /gene="galM"
                     /locus_tag="OHO85_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCQ2670972.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(17585..18733)
                     /gene="galK"
                     /locus_tag="OHO85_001590"
     CDS             complement(17585..18733)
                     /gene="galK"
                     /locus_tag="OHO85_001590"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCQ2670973.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18737..19783)
                     /gene="galT"
                     /locus_tag="OHO85_001591"
     CDS             complement(18737..19783)
                     /gene="galT"
                     /locus_tag="OHO85_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCQ2670974.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19793..20809)
                     /gene="galE"
                     /locus_tag="OHO85_001592"
     CDS             complement(19793..20809)
                     /gene="galE"
                     /locus_tag="OHO85_001592"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCQ2670975.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(21070..22542)
                     /gene="modF"
                     /locus_tag="OHO85_001593"
     CDS             complement(21070..22542)
                     /gene="modF"
                     /locus_tag="OHO85_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCQ2670976.1"
                     /translation="MSSLQILQGAFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            complement(22610..23398)
                     /gene="modE"
                     /locus_tag="OHO85_001594"
     CDS             complement(22610..23398)
                     /gene="modE"
                     /locus_tag="OHO85_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCQ2670977.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VIMSLPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            23527..23676
                     /gene="acrZ"
                     /locus_tag="OHO85_001595"
     CDS             23527..23676
                     /gene="acrZ"
                     /locus_tag="OHO85_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCQ2670978.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23843..24616
                     /gene="modA"
                     /locus_tag="OHO85_001596"
     CDS             23843..24616
                     /gene="modA"
                     /locus_tag="OHO85_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2670979.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVAIFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            24616..25305
                     /gene="modB"
                     /locus_tag="OHO85_001597"
     CDS             24616..25305
                     /gene="modB"
                     /locus_tag="OHO85_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCQ2670980.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            25308..26366
                     /gene="modC"
                     /locus_tag="OHO85_001598"
     CDS             25308..26366
                     /gene="modC"
                     /locus_tag="OHO85_001598"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCQ2670981.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(26367..27185)
                     /gene="ybhA"
                     /locus_tag="OHO85_001599"
     CDS             complement(26367..27185)
                     /gene="ybhA"
                     /locus_tag="OHO85_001599"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCQ2670982.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            27340..28335
                     /gene="pgl"
                     /locus_tag="OHO85_001600"
     CDS             27340..28335
                     /gene="pgl"
                     /locus_tag="OHO85_001600"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCQ2670983.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYSIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(28376..29329)
                     /locus_tag="OHO85_001601"
     CDS             complement(28376..29329)
                     /locus_tag="OHO85_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2670984.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            29513..30565
                     /locus_tag="OHO85_001602"
     CDS             29513..30565
                     /locus_tag="OHO85_001602"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCQ2670985.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            30641..32074
                     /locus_tag="OHO85_001603"
     CDS             30641..32074
                     /locus_tag="OHO85_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCQ2670986.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPQVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            32257..34518
                     /locus_tag="OHO85_001604"
     CDS             32257..34518
                     /locus_tag="OHO85_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCQ2670987.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYKIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGYTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(34752..36035)
                     /locus_tag="OHO85_001605"
     CDS             complement(34752..36035)
                     /locus_tag="OHO85_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCQ2670988.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(36187..36663)
                     /locus_tag="OHO85_001606"
     CDS             complement(36187..36663)
                     /locus_tag="OHO85_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCQ2670989.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(36722..38011)
                     /gene="bioA"
                     /locus_tag="OHO85_001607"
     CDS             complement(36722..38011)
                     /gene="bioA"
                     /locus_tag="OHO85_001607"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCQ2670990.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGKR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            38098..39138
                     /gene="bioB"
                     /locus_tag="OHO85_001608"
     CDS             38098..39138
                     /gene="bioB"
                     /locus_tag="OHO85_001608"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCQ2670991.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            39135..40289
                     /gene="bioF"
                     /locus_tag="OHO85_001609"
     CDS             39135..40289
                     /gene="bioF"
                     /locus_tag="OHO85_001609"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCQ2670992.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIAADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQMVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            40276..41031
                     /gene="bioC"
                     /locus_tag="OHO85_001610"
     CDS             40276..41031
                     /gene="bioC"
                     /locus_tag="OHO85_001610"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCQ2670993.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPKGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            41024..41701
                     /gene="bioD"
                     /locus_tag="OHO85_001611"
     CDS             41024..41701
                     /gene="bioD"
                     /locus_tag="OHO85_001611"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCQ2670994.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT        10017 a        10373 c        11157 g        10295 t
ORIGIN      
        1 atgtataagt cttggaaatg ggcatcaaaa agagataaat tgttctcgat caaattggct
       61 gaaaggcggt aatttagcta taaattgatc accgtcgaaa aatgcaaatt tgcttcaaca
      121 aaaacctgtt tattgtaagg attttgcggc gtaatatata cgtgggatca atttgagttt
      181 ttattaacat gtttgcaacc tttctttacg ccgtttttgt gtgcattcac atggtatgat
      241 gaaagtgttc aaacaaattt ctattggggc atgcgtgtga ccctttctaa cggggttcac
      301 tctcggagtc ttcatgcgat gagcaaggag tcatgatgtt agatatagtc gaactgtcgc
      361 gcttacagtt tgccttgacc gcgatgtacc acttcctttt tgtgccactg acgctcggta
      421 tggcgttcct gctggccatt atggaaacgg tctacgtcct ctccggcaaa cagatttata
      481 aagatatgac caagttctgg ggcaagttgt ttggtatcaa cttcgctctg ggggtggcta
      541 ccggtctgac catggagttc cagttcggga ctaactggtc ttactattcc cactatgtag
      601 gggatatctt cggtgcgccg ctggcaatcg aaggtctgat ggccttcttc ctcgaatcca
      661 cctttgtagg tctgttcttc ttcggttggg atcgtctggg taaagttcag catatgtgtg
      721 tcacctggct ggtggcgctc ggttcaaacc tgtccgcact gtggattctg gttgcgaacg
      781 gctggatgca aaacccaatc gcgtccgatt tcaactttga aactatgcgt atggagatgg
      841 tgagcttctc cgagctggtg cttaacccgg ttgctcaggt gaaattcgtt cacactgtag
      901 cgtctggtta tgttactggc gcgatgttca tcctcggtgt cagcgcatgg tatatgctga
      961 aaggtcgtga cttcgccttc gctaaacgct cctttgctat cgctgccagc ttcggtatgg
     1021 ctgctgttct gtctgttatt gttttgggtg atgaatccgg ctacgaaatg ggcgacgtgc
     1081 agaaaaccaa actggctgct attgaagccg agtgggaaac gcaacctgcg cctgctgcct
     1141 ttactctgtt cggcattcct gatcaggaag aggagacgaa caaatttgcg attcagatcc
     1201 cttacgcact gggcatcatt gcaacgcgtt ccgtggatac cccggttatc ggcctgaaag
     1261 agctgatggt gcagcatgaa gaacgcattc gtaacgggat gaaggcgtac tctctgctcg
     1321 aacaactgcg ttctggttct accgaccagg cggttcgtga ccagttcaat agcatgaaga
     1381 aagacctcgg ttacggtctg ctgctgaaac gctatacgcc aaacgtggct gatgcgactg
     1441 aagcgcagat tcaacaggca accaaagact ccatcccgcg tgtagcgccg ctgtactttg
     1501 cgttccgtat catggtggcg tgtggcttcc tgcttctggc aatcatcgcg ctctctttct
     1561 ggagtgtcat ccgcaaccgc attggcgaga aaaaatggct tctgcgcgcc gcgctgtacg
     1621 gtattccgct gccgtggatt gctgtagaag cgggctggtt cgtggctgaa tatggccgcc
     1681 aaccgtgggc tatcggtgaa gtgctgccga cagctgtggc gaactcgtca ctgaccgcag
     1741 gcgatctcat cttctcaatg gtgctgattt gcggcctgta taccctgttc ctggtggcag
     1801 aattgttctt aatgttcaaa tttgcacgcc tcggcccaag cagcctgaaa accggtcgct
     1861 atcactttga gcagtcttcc acgactactc agccggcacg ctaagacagg agtcgtcaaa
     1921 tgatcgatta tgaagtattg cgttttatct ggtggctgct ggttggcgtt ctgctgattg
     1981 gttttgcagt cactgacggt ttcgacatgg gggtgggcat gctcacccgt ttcctcggtc
     2041 gtaacgacac cgagcgtcga attatgatta actccattgc accacactgg gacggtaacc
     2101 aggtttggct gatcaccgcg ggcggcgcac tctttgctgc ctggccgatg gtctatgccg
     2161 ctgcgttctc cggcttctat gtggcgatga tcctcgtgct ggcgtctttg ttcttccgtc
     2221 cggtcggttt tgactaccgc tccaagattg aagaaacccg ctggcgtaac atgtgggact
     2281 ggggcatctt cattggtagc ttcgttccgc cgctggtaat tggtgtagcg ttcggtaacc
     2341 tgttgcaggg cgtaccgttc aacgttgatg aatatctgcg tctgtactac accggtaact
     2401 tcttccagtt gcttaacccg ttcggcctgc tggcaggcgt ggtgagcgta gggatgatca
     2461 ttactcaggg cgcaacctat ctgcaaatgc gtaccgtggg cgaactgcac ctgcgtaccc
     2521 gtgcaacggc tcaggtggct gcgctggtga cactggtctg tttcgcactg gctggcgtat
     2581 gggtgatgta cggtatcgat ggttatgtcg tgaaatcgac aatggaccat tacgcagcct
     2641 ctaacccact gaataaagaa gtggttcgtg aagctggcgc atggctggtt aacttcaaca
     2701 acacgccaat tctgtgggct attccggcac tgggtgtggt tctgccgctg ctgaccatcc
     2761 tgactgcacg tatggataaa gccgcgtggg cgtttgtgtt ctcctccctg acgctggcct
     2821 gcatcatcct gacagccggt atcgcaatgt tcccgtttgt gatgccgtcc agcaccatga
     2881 tgaacgcaag tctgacaatg tgggatgcaa cttccagcca gctgacgctt aacgtcatga
     2941 cctgggttgc ggtggttctg gtaccgatca ttctgctcta caccgcctgg tgttactgga
     3001 aaatgttcgg tcgtatcacc aaagaagata ttgaacgtaa cacccactct ctgtactaag
     3061 taaggagcta aaaatgtggt atttcgcatg gattctggga acgcttcttg cctgttcgtt
     3121 tggggtaatc accgcgctgg cgcttgaaca cgtcgaatca ggcaaagccg gtcaagaaga
     3181 catctgatga gtaagattat cgcgactttg tatgcggtaa tggacaagcg ccccctgcgg
     3241 gcgctttcct tcgtgatggc gcttctgtta gcaggatgta tgttttggga cccatctcgt
     3301 tttgccgcga agaccagtga actggaaatc tggcatggtt tattgctgat gtgggccgtc
     3361 tgtgctggtg tgattcacgg cgtgggcttt cgtccgcaga aggttctttg gcaagggatt
     3421 ttttgcccat tgcttgccga tattgttctc attgtcgggc tgattttctt cttcttttaa
     3481 atcagaattc tctttaaaaa attatgggcc gctccaggcc cataaatttt tacgctccct
     3541 taacttgccc tcattcccaa acctcaatcg cgcgcgtata gtagcagcgt ttaaaagctc
     3601 taacttttgt tgcattaccg ggatgtaaag tgaatacaac gctgtttcga tggccggttc
     3661 gcgtctacta tgaagatacc gatgccggtg gtgtggtgta ccacgccagt tacgtcgctt
     3721 tttatgaaag agcacgcaca gagatgctgc gtcatcatca cttcagtcag caggcgctga
     3781 tggctgaacg cgttgccttt gtggtacgta aaatgacggt ggaatattac gcacctgcgc
     3841 ggctcgacga tatgctcgaa atacagactg aaataacatc aatgcgtggc acctctttgg
     3901 ttttcacgca acgtattgtc aacgccgaga atactttgct gaatgaagca gaggttctgg
     3961 ttgtttgcgt tgacccactc aaaatgaagc ctcgtgcgct tcccaagtct attgtcgcgg
     4021 agtttaagca gtgactgaca tgaatatcct tgatttgttc ctgaaggcta gccttctggt
     4081 taaacttatc atgttgattt tgattggttt ttcaatcgca tcttgggcca ttattatcca
     4141 gcggacccgt attcttaacg cagcggcgcg cgaagccgaa gcgtttgaag ataaattctg
     4201 gtctggaatc gaactctctc gcctctatca agagagccag gggaaacggg ataatctgac
     4261 tggttcggaa caaatctttt acagcgggtt caaagagttt gtgcgcctgc atcgtgccaa
     4321 tagccatgcg ccggaagccg tagtggaagg ggcgtcgcgt gctatgcgta tctccatgaa
     4381 ccgtgaactt gaaaatctgg aaacgcacat tccgttcctc ggtacggttg gctccatcag
     4441 cccgtatatt ggtctgtttg gtacggtctg ggggatcatg cacgccttta tcgccctcgg
     4501 ggcggtaaaa caagcaacac tgcaaatggt tgcgcccggt atcgcagaag cgttgattgc
     4561 gactgcaatt ggtctgttcg ccgcaattcc ggcggtaatg gcttataacc gcctcaacca
     4621 gcgcgtaaac aaactggaac tgaattacga caactttatg gaagagttta ccgcgattct
     4681 gcaccgccag gcgtttaccg ttagcgagag caacaagggg taagccatgg ccagagcgcg
     4741 tggacgaggt cgtcgcgatc tcaagtccga aatcaacatt gtaccgttgc tggacgtact
     4801 gctggtgctg ttgctgatct ttatggcgac agcgcccatc atcacccaga gcgtggaggt
     4861 cgatctgcca gacgctactg aatcacaggc ggtgagcagt aacgataatc cgccagtgat
     4921 tgttgaagtg tctggtattg gtcagtacac cgtggtggtt gagaaagatc gcctggagcg
     4981 tttaccacca gagcaggtgg tggcggaagt gtccagccgt ttcaaggcca acccgaaaac
     5041 ggtctttctg atcggtggcg caaaagatgt gccttacgat gaaataatta aagcactgaa
     5101 cttgttacat agtgcgggtg tgaaatcggt tggtttaatg acgcagccta tctaaacatc
     5161 tgcgtttccc ttgcttgaaa gagagcgggt aacaggcgaa cagtttttgg aaaccgagag
     5221 tgtcaaaggc aaccgaacaa aacgacaagc tcaagcgggc gataattatt tcagcagtgc
     5281 tgcatgtcat cttatttgcg gcgctgatct ggagttcgtt cgatgagaat atagaagctt
     5341 cagccggagg cggcggtggt tcgtctatcg acgctgtcat ggttgattca ggtgcggtag
     5401 ttgagcagta caaacgcatg caaagccagg aatcaagcgc gaagcgttct gatgaacagc
     5461 gcaagatgaa ggaacagcag gctgctgaag aactgcgtga gaaacaagcg gctgaacagg
     5521 aacgcctgaa gcaacttgag aaagagcggt tagcggctca ggagcagaaa aagcaggctg
     5581 aagaagccgc aaaacaggcc gagttaaagc agaagcaagc tgaagaggcg gcagcgaaag
     5641 cggcggcaga tgctaaagcg aaggccgaag cagatgctaa agctgcggaa gaagcagcga
     5701 agaaagcggc tgcagacgca aagaaaaaag cagaagcaga agccgccaaa gccgcagccg
     5761 aagcgcagaa aaaagccgag gcagccgctg cggcactgaa gaagaaagcg gaagcggcag
     5821 aagcagctgc agctgaagca agaaagaaag cggcaactga agctgctgaa aaagccaaag
     5881 cagaagctga gaagaaagcg gctgctgaaa aggctgcagc tgataagaaa gcggcagcag
     5941 agaaagctgc agccgacaaa aaagcagcag aaaaagcggc tgctgaaaag gcagcagctg
     6001 ataagaaagc agcggcagaa aaagccgccg cagacaaaaa agcggcagcg gcaaaagctg
     6061 cagctgaaaa agccgctgca gcaaaagcgg ccgcagaggc agatgatatt ttcggtgagc
     6121 taagctctgg taagaatgca ccgaaaacgg ggggaggggc gaaagggaac aatgcttcgc
     6181 ctgccgggag tggtaatact aaaaacaatg gcgcatcagg ggccgatatc aataactatg
     6241 ccgggcagat taaatctgct atcgaaagta agttctatga cgcatcgtcc tatgcaggca
     6301 aaacctgtac gctgcgcata aaactggcac ccgatggtat gttactggat atcaaacctg
     6361 aaggtggcga tcccgcactt tgtcaggctg cgttggcagc agctaaactt gcgaagatcc
     6421 cgaaaccacc aagccaggca gtatatgaag tgttcaaaaa cgcgccattg gacttcaaac
     6481 cgtaatcgcg atgttgactg ttcggacggt caacatcagg caccggttgc cacggggttc
     6541 tggtagtttt gtgtatttta gtttgttaac attctgctaa attatcgtgg gccatcggtc
     6601 cagataaggg agatatgatg aagcaggcat tacgagtagc atttggtttt ctcatactgt
     6661 gggcatcagt tctgcatgct gaagtccgca ttgtgatcga cagcggtgta gattccggtc
     6721 gtcctattgg tgttgttcct ttccagtggg cggggcctgg tgcggcacct gaagatattg
     6781 gcggcatcgt tgctgctgac ttgcgtaaca gcggtaaatt taatccgtta gatcgcgctc
     6841 gtctgccaca gcagccgggt agtgcgcagg aagtacaacc agctgcatgg tccgcactgg
     6901 gcattgacgc tgtagttgtc ggtcaggtca ctccgaatcc ggatggttct tacaatgttg
     6961 cttatcaact tgttgacact ggcggcgcac cgggtactgt acttgctcag aactcgtaca
     7021 aagtgaacaa gcagtggctg cgttatgctg gtcataccgc cagtgatgaa gtgtttgaaa
     7081 aactgaccgg cattaaaggt gcgttccgta ctcgtattgc ctacgttgtt cagaccaacg
     7141 gcggtcagtt cccgtatgaa ctgcgcgtat ctgactatga cggttacaac cagtttgtcg
     7201 ttcaccgttc accgcagccg ctgatgtctc cggcgtggtc accagacggt tctaaactgg
     7261 cttatgtgac cttcgaaagc ggtcgttccg cgctggttat tcagacgctg gcaaatggcg
     7321 ctgtacgtca ggtggcttca ttcccgcgtc acaacggtgc acctgcattc tcgccagacg
     7381 gcagcaaact ggcattcgcc ttgtcgaaaa ccggtagtct gaacctgtac gtaatggatt
     7441 tggcttctgg tcagatccgc caggtgactg atggtcgcag taacaatacc gaaccgacct
     7501 ggttcccgga tagccagaac ctggcattta cttctgacca ggccggtcgt ccgcaggttt
     7561 ataaagtgaa tatcaacggc ggtgcgccac aacgtattac ctgggaaggt tcgcagaacc
     7621 aggatgcgga tgtcagcagc gacggtaaat ttatggtaat ggtcagctcc aatggtgggc
     7681 agcagcacat tgccaaacaa gatctggcaa cgggaggcgt acaagttctg tcgtccacgt
     7741 tcctggatga aacgccaagt ctggcaccta acggcactat ggtaatctac agctcttctc
     7801 aggggatggg atccgtgctg aatttggttt ctacagatgg gcgtttcaaa gcgcgtcttc
     7861 cggcaactga tggacaggtc aaattccctg cctggtcgcc gtatctgtga taataattaa
     7921 ttgaatagta aaggaatcat tgaaatgcaa ctgaacaaag tgctgaaagg gctgatgatt
     7981 gctctgcctg ttatggcaat tgcggcatgt tcttccaaca agaacgccag caatgacggc
     8041 agcgaaggca tgctgggtgc cggcactggt atggatgcga acggcggcaa cggcaacatg
     8101 tcttccgaag agcaggctcg tctgcaaatg caacagctgc agcagaacaa catcgtttac
     8161 ttcgatctgg acaagtacga tatccgttct gacttcgctc aaatgctgga tgcacatgca
     8221 aacttcctgc gtagcaaccc gtcttacaaa gtcaccgtag aaggtcacgc ggacgaacgt
     8281 ggtactccgg aatacaacat ctccctgggt gaacgtcgtg cgaacgccgt taagatgtac
     8341 ctgcagggta aaggcgtttc tgcagaccag atctccatcg tttcttacgg taaagaaaaa
     8401 cctgcagtac tgggtcatga cgaagcggca tactccaaaa accgtcgtgc ggtactggtt
     8461 tactaagaga attgcatgag cagtaacttc agacatcaac tattgagtct gtcgttactg
     8521 gttggtatag cggccccctg ggccgctttt gctcaggcac caatcagtag tgtcggctca
     8581 ggctcggtcg aagaccgcgt cactcaactt gagcgtattt ctaatgctca cagccagctt
     8641 ttaacccaac tccagcaaca actttctgat aatcaatccg atattgattc cctgcgtggt
     8701 cagattcagg aaaatcagta tcaactgaat caggtcgtgg agcggcaaaa gcagatcctg
     8761 ttgcagatcg acagcctcag cagcggtggt gcagcggcgc aatcaaccag cagcgatcaa
     8821 agcggtgcgg cggcatcaac gacgccgaca gctgatgctg gtactgcgaa tgctggcgcg
     8881 ccggtgaaaa gcggcgatgc aaacacggat tacaatgcag ctattgcgct ggtgcaggat
     8941 aaatcccgcc aggatgacgc aatggtggca tttcagaatt tcatcaaaaa ttaccctgat
     9001 tcaacttacc tgccaaacgc caattattgg ctgggtcagt taaactacaa caagggtaaa
     9061 aaagatgatg cggcgtacta ttttgcttcg gtagtgaaaa actatccgaa gtcaccaaag
     9121 gctgctgatg cgatgtttaa agtcggcgtc atcatgcagg acaaaggtga caccgcaaaa
     9181 gcgaaagccg tataccagca ggttatcagt aaataccctg gtactgatgg cgctaaacag
     9241 gcacaaaaac gtctgaacgc gatgtaatgc ataacacacg accagaagtc gcattatttc
     9301 tggtcgtgtc gtgcgaatca taagcagttg agtgatctgc atcgaaattt ttgttgcgct
     9361 caagtctgaa atcagtaata tatgccgccg ttgccacggg atatcaaaca aaccgaaagc
     9421 aacgaaaaag tgggtcgtta gctcagttgg tagagcagtt gacttttaat caattggtcg
     9481 caggttcgaa tcctgcacga cccaccaatc gctaaggtgg aagcggtagt aaaacgtgaa
     9541 ggataacgtt gcatcagcaa cggcccgaag ggcgagacga agtcgagtca tcctgcacga
     9601 cccaccacta acatagttag ttgtagtatc cagcgtagta ttgggtgatt agctcagctg
     9661 ggagagcacc tcccttacaa ggagggggtc ggcggttcga tcccgtcatc acccaccacc
     9721 gggtcgttag ctcagttggt agagcagttg acttttaatc aattggtcgc aggttcgaat
     9781 cctgcacgac ccaccagttt tagcatcgaa gacagatgtt aagcgtgtag gataacgttg
     9841 cgtcagcaac ggcccgtagg gcgagcgaag cgagtcatcc tgcacgaccc accactaatt
     9901 acggtgggtt cggtggaagt agtttgtagt atccagcgca gtatcgggtg attagctcag
     9961 ctgggagagc acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc
    10021 actcgggtcg ttagctcagt tggtagagca gttgactttt aatcaattgg tcgcaggttc
    10081 gaatcctgca cgacccacca attttaacat caaactcaga tgttaagcgt gaaggataac
    10141 gttgcgccag caacggcccg tagggcaagc gaagcgagtc atcctgcacg acacaccaat
    10201 cttaaagatt ggccccgagt agaaatcttt caggtaacac ccgtatgggt cgttagctca
    10261 gttggtagag cagttgactt ttaatcaatt ggtcgcaggt tcgaatcctg cacgacccac
    10321 catcctgaat gattaaagca gcataatccc gcaaggggtc gttagctcag ttggtagagc
    10381 agttgacttt taatcaattg gtcgcaggtt cgaatcctgc acgacccacc aatgtaaaaa
    10441 agcgccctaa aggcgctttt ttgctatctg cgatactcaa agattcgaac ctgcagcagg
    10501 tttgagttga gcgcagcgaa acaacggagc cgctcgcggc gacggcccga agggcgagcg
    10561 aagcgagtca tcctgcacga cccaccaatg taaaaaagcg ccctaaaggc gcttttttgc
    10621 tattcaggca tcctcaattt cactttgtaa acctgatgac atcgtcagag cttactgtgc
    10681 aagcaactct acgtcggtgg aattaggcgt aattcgagtg acttttcccc accattcgac
    10741 tatcttgttt agcatataaa acaaattaca ccgataacag cgaatattac gctaatgtcg
    10801 gttttaacgt taagcctgta aaacgagatg gtaagatgag cgtaatgttt gatccagaca
    10861 cggcgattta tcctttcccc ccgaagccga cgccgttaag cattgatgaa aaagcgtatt
    10921 accgcgagaa gataaaacgt ctgctaaaag aacgtaatgc ggtgatggtt gcccactact
    10981 ataccgatcc cgaaattcaa caactggcag aagaaaccgg tggctgtatt tctgattctc
    11041 tggaaatggc gcgcttcggt gcaaagcatc ccgcttctac tttgttagtc gctggggtga
    11101 gatttatggg agaaaccgcc aaaattctca gtccggaaaa aacaattctg atgccgacac
    11161 ttcaggctga atgttcactg gatctcggct gccctgttga agaatttaac gcattttgcg
    11221 atgcccatcc cgatcgtact gtcgtcgtct acgccaacac ttctgctgcg gtaaaagcgc
    11281 gcgcagattg ggtggtaact tcaagcattt ccgtcgaact tattgatcat cttgatagtt
    11341 tgggtgaaaa aatcatctgg gcacccgaca aacatctggg gcgttacgtg caaaaacaga
    11401 cgggtggaga cattctatgc tggcagggtg cctgtattgt gcatgatgaa tttaagactc
    11461 aggcgttaac ccgcttgcaa gaagaatacc cggatgctgc catactggtg catccagaat
    11521 caccacaagc tattgtcgat atggcggatg cggtcggttc caccagtcaa ctgatcgctg
    11581 ctgcgaaagc attgccacat cagcggctta tcgtggcaac cgatcggggc attttctaca
    11641 aaatgcagca ggcagtgcca gataaagagt tactggaagc accaaccgca ggtgagggtg
    11701 caacctgccg cagctgcgcg cattgtccgt ggatggccat gaatggcctt cagtccatcg
    11761 cagaggcatt agaacaggaa ggaagcaatc acgaggttca tgttgatgaa aggctgcgag
    11821 agagggcgct ggtgccgctc aatcgtatgc tggattttgc ggctacacta cgtggataac
    11881 gaataataag gcgtaacgtt acgctttggg ggaaagatgg atttttttag tgtgcagaat
    11941 atcctggtac atataccaat aggggcaggc ggttatgatc tctcatggat cgaagcggta
    12001 ggcacgatcg ccgggttgct gtgtattggc cttgccagtc tggagaagat cagcaactac
    12061 ttctttggcc tgatcaacgt caccttgttt ggcattattt tctttcagat tcagctgtat
    12121 gccagcctgc tattacaggt gtttttcttt gccgcgaata tttacggttg gtatgcgtgg
    12181 tcgcgacaaa ccagtcagaa cgaggcggag ttgaaaattc gctggttgcc attgccgaag
    12241 gcactcagct ggttggcggt ttgcgttgtt tcgattggtc tgatgacggt atttatcaat
    12301 ccggtgtttg catttttgac ccgcgtggca gtcatgatca tgcaagcatt aggattacag
    12361 gttgtgatgc ctgaactgca accggacgct ttcccgttct gggattcatg catgatggtg
    12421 ttatctatcg tggcaatgat tctgatgacg cgtaagtatg tggaaaactg gctgttgtgg
    12481 gtgattatta acgtgattag cgtcgttatt tttgcacttc agggcgttta cgccatgtct
    12541 ctggagtaca tcatcctgac ctttattgcg ctcaacggca gccggatgtg gatcaacagc
    12601 gcacgtgaaa gaggctcacg cgcgctgtcc cattaatggt gatgatgtga atggccagac
    12661 accccttcat tcaggtggca gtctggccca tgacaaggtt gatattccat ctgaatggtg
    12721 gcgtgctcaa tctgatagtg atccatcagg tagtgttgga tctgatccaa caaggcatcg
    12781 tgatcgtgtg gcgggatcac ctgcacatgc aacgtcatca ccggcttctc gcctaccatc
    12841 catacatgta catggtgcac attgcggact tccgggattt cccggcacat acgacgcttc
    12901 agttcagcga tatccagcga taccggtgca ccttcaagta attcattcac gctatctttc
    12961 aacagtcgcc acgcgctacg cagaaccaga agcgacacca gtatcgagag aatggggtca
    13021 gcaggtgtcc agccagtcca gataataatc aacgcggcaa taatagcccc aaccgaaccc
    13081 agcaggtccc cgagcacatg cagtgccgct gctcgtacgt tgaggttttt ctcttcgctg
    13141 ccgtgatgaa gtaaccagaa agaaagtata tttgccagca acccagccac ggcaattgcc
    13201 atcatcatgc cgccctcgac cggacgcggc gtgcggaagc gttctatcgc ctcccagaca
    13261 atcaaaatgg taattaccac gagggctatg gcattcacaa acgccgcgag cgtggtcagt
    13321 cttagccaac cgaaagtgtg gcgaatggtg ggaggacgac gtgaaaattg cacggcgagt
    13381 agggcgaaaa gcagggcggc agtatcggtc aacatatgac ccgcatcggc cagcaatgcc
    13441 agagaaccag aaagaaaacc accaacgact tctaccagca taaacccggc agtcacgccg
    13501 aaagcataca acaagcgacg agcattatta tcttctggca ggtgtgagga cgtgtgtgag
    13561 tgtgagtgcg ccatgacgag tcatccctta attattctca cttaacttta tgacatcata
    13621 ctgcttttag aagtgaaaaa ttaaaaggga gagactccgc tctcccatta ttggctattt
    13681 tgcagggtta ctgcgtggta ccgtcggttt tggtatcgac atcattattg atgccatcac
    13741 cggtttgtac ctttttattg atatccggac agcgaccatc tttgcacatg gtgttcttgt
    13801 gctcttcatc tttggtcatt ccgtcattgt tcattgaaga accatccgaa tgcagcattg
    13861 tgccaccaga accggtattt accccgttat tgtcgacgtt atttggcgcg acattttcac
    13921 gggcgtcagg ggctacctgg cccgcatcag ctgcggcgtt tgcctggccg ttattagttt
    13981 gcgctccgct atcggcagcc agtgcggcac cgctggcaag gcttagagtg gcagtcagaa
    14041 ataatgtggc cagttttgtc attttcatag gatgctcctg ttatggtcgt tatgtcggat
    14101 aacctcttcc aacagtgcat ttgcaggtga atataaggca ttggtttaag atttcagcca
    14161 ggttatgaaa cgcagcagag aatcttgaaa taattaacaa acaaaggagt tacagttaga
    14221 aattgtagga gagatctcgt ttttcgcgac aatctggcgt ttttcttgct aattccagga
    14281 ttaatccgtt catagtgtaa aaccccgttt acacattctg acggaagata tagattggaa
    14341 gtattgcatt cactaagata agtatggcaa cactggaaca gacatgaatt atcagaacga
    14401 cgatttacgt atcaaagaaa tcaaagagtt acttcctcct gtcgcattgc tggaaaaatt
    14461 ccccgctact gaaaatgccg cgaatacggt tgcccatgcc cgaaaagcga tccataagat
    14521 cctgaaaggt aatgatgatc gcctgttggt ggtgattggc ccatgctcaa ttcatgatcc
    14581 tgtcgcagca aaagagtatg ccactcgctt gctggcgctg cgtgaagagc tgaaagatga
    14641 gctggaaatc gtaatgcgcg tctattttga aaagccgcgt accacggtgg gctggaaagg
    14701 gctgattaac gatccgcata tggataatag cttccagatc aacgacggtc tgcgtatagc
    14761 ccgtaaattg ctgcttgata ttaacgacag cggtctgcca gcggcaggtg agtttctcga
    14821 tatgatcacc ccacaatatc tcgctgacct gatgagctgg ggcgcaattg gtgcacgtac
    14881 caccgaatcg caggtgcacc gcgaactggc gtctggtctt tcttgtccgg taggttttaa
    14941 aaatggcaca gacggtacga ttaaagtggc tatcgatgcc attaatgccg ccggtgcgcc
    15001 gcactgcttc ctgtccgtaa ctaaatgggg gcattcggcg attgtgaata ccagcggtaa
    15061 cggtgattgc catatcattc tgcgcggcgg taaagagcct aactacagcg cgaagcacgt
    15121 tgctgaagtg aaagaagggc tgaacaaagc aggcctgcca gcacaggtga tgatcgattt
    15181 cagccatgct aactcgtcca aacaattcaa aaagcagatg gatgtttgtg ctgacgtttg
    15241 ccagcagatt gccggtggcg aaaaggccat tattggcgtg atggtggaaa gccatctggt
    15301 ggaaggcaat cagagcctcg atagcgggga gccgctggcc tacggtaaga gcatcaccga
    15361 tgcctgcatt ggctgggaag ataccgatgc tctgttacgt caactggcga atgcagtaaa
    15421 agcgcgtcgc gggtaaggtt taattgtcgg atgcgccgtc agagtggcgt atccgatgaa
    15481 tcaccacagg cctgataagt cgcgcagcgt cgcatcaggc aatgtgctcc attgttagca
    15541 acaaaaaagc cgactcactt gcagtcggct ttctcatttt aaacgaatga cgtttacttc
    15601 gctttaccct ggtttgcaac cgccgctgct ttcgctgcga tctcgtcagc attacccaga
    15661 taatagcgtt tcagcggttt gaaattctcg tcgaactcat acaccagcgg cacgccagtc
    15721 gggatattaa gctcaagaat ctcttcttcg ctcatgttat caagatattt caccagcgca
    15781 cgtaaagagt taccgtgtgc agcgatgatc acgcgctcac cgctcttcat acgcggcaga
    15841 atagtttcat tccagtaagg gatcacgcgg tcaatggtca gcgccaggct ttccgtcagc
    15901 ggcagttctt tttcgctcag tttcgcgtaa cgcggatcgt gacccggata acgctcatca
    15961 tctttagtca gttccggcgg agtcactgca aaaccacgac gccactgttt cacctgctcg
    16021 tcgccatact tttcagcagt ttccgctttg ttcagaccct gcaacgcacc gtagtgacgt
    16081 tcgttcagtt tccaggattt ctcaacgggc agccatgcct gatccagttc gtccagcaca
    16141 ttccacaggg tatggatagc gcgtttcagc acagaggtgt aagcaaagtc aaagctgtaa
    16201 ccttcctctt tcagcagctt acctgctgct tttgcttcgc ttacgccttt ctcagacaga
    16261 tccacgtcgt accaaccggt gaaacggttt tctttgttcc actgactttc gccatgacga
    16321 accagaacca gcttagttac agccatatac ttactcctca aatcattttt taatgataat
    16381 aattctcatt atattgccgt gacgaagcaa cagcaatgct tacgcataac catagcgaaa
    16441 atagtgtcgc agtgtaaggt tgttgtgaat attgagttgc aaatatgtcg gtgtttgctg
    16501 gtgatttgag caatataagg ataaagccct cattacgagg gcttcaggtg ctactcagca
    16561 ataaactgat attccgtcag gctggaatac tcttcgccag gacgcaggaa gcagtccggt
    16621 tgcggccatt cagggtggtt tgggctgtcc ggcagaaact cgctttccag tgccaggcct
    16681 tgccagtcgg cgtaaggttc agttcctctt gatggcgtgc cgccgaggaa gttgccggag
    16741 tagaattgca gagccggagc ggtggtgtag accttcagct gcaatttttc atctgctgac
    16801 cagacatgcg ccgccacttt cttgccatcg cctttggcct gtaacaagaa tgcgtgatcg
    16861 tagcctttca ctttgcgctg atcgtcgtcg gcaagaaact cactggcgat gattttggcg
    16921 ctacggaaat caaaagacgt tccggcgaca gatttcaggc cgtcgtgcgg aatgccgcct
    16981 tcatcaaccg gcagatattc gtccgccaga atctgcaact tgtgattgcg cacgtcagac
    17041 tgctcgccgt caagattgaa atagacgtga ttagtcatat tcaccgggca aggtttatca
    17101 actgtggcgc gataagtaat agagatacgg ttatcgtcgg tcagacgata ttgcaccgtc
    17161 gcgccgagat tacccgggaa gccctgatcg ccatcatctg aactcagggc aaacagcacc
    17221 tgacgatcgt tctggttcac aatctgccag cgacgtttgt cgaacccttc cgggccgccg
    17281 tgcagctggt taacgccctg acttggcgaa agcgtcacgg tttcaccgtc aaaggtataa
    17341 cggctattgg cgatgcggtt ggcataacga ccaatagagg cccccagaaa cgcagcctga
    17401 tcctgatagc attccgggct ggcacagccg agcagcgcct cgcggacgct gccatcggaa
    17461 agcggaatac gagctgaaag taaagtcgca ccccagtcca tcagcgtgac taccatccct
    17521 gcgttgttac gcaaagttaa cagtcggtac ggctgaccat cgggtgccag tgcgggagtt
    17581 tcgttcagca ctgtcctgct ccttgtgatg gtttacaaac gtaaaaagtc tctttaatac
    17641 ctgtttttgc ttcatattgt tcagcgacag cttgctgtac ggcaggcacc agctcttccg
    17701 ggatcagcgc gacgatacag ccgccaaatc cgccgccggt catgcgtacg ccacctttgt
    17761 cgccaatcac agctttgacg atttctacca gagtgtcaat ttgcggcacg gtgatttcga
    17821 aatcatcgcg catagaggca tgagattccg ccatcaactc gcccatacgt ttcaggtcgc
    17881 cttgctccag cgcgctggca gcttcaacgg tgcgggcgtt ttcagtcagg atatgacgca
    17941 cgcgtttcgc cacgattggg tccagctcat gtgcaacagc gttgaactct tcaatggtga
    18001 catcgcgcag agctggctgc tggaagaaac gcgcaccggt ttcgcactgt tcacgacggg
    18061 tgttgtattc gctgccaacc agggtacgtt tgaagttact gttgatgatg acgacagcca
    18121 cacctttcgg catggaaact gctttggtcc ccagtgaacg gcagtcaatc agcaaggcat
    18181 gatctttctt gccgagcgcg gaaattagct gatccatgat cccgcagtta cagccaacaa
    18241 actggttttc tgcttcctga ccgttaagcg cgatttgtgc gccgtccagc ggcagatgat
    18301 aaagctgctg caatacggtt ccgaccgcca cttcgagtga ggcggaagaa cttaacccgg
    18361 caccctgcgg cacattgccg ctgatcacca tgtccacgcc gccgaagctg ttgttacgca
    18421 gttgcagatg tttcaccacg ccacgaacgt agttagccca ttgatagttt tcatgtgcga
    18481 caatgggcgc atcgagggaa aactcgtcga gctgattttc ataatcggct gccatcacgc
    18541 gaactttacg gtcatcgcgt ggtgcacaac tgatcacggt ttgataatca atcgcgcagg
    18601 gcagaacgaa accgtcgttg tagtcggtgt gttcaccaat caaattcacg cggccaggcg
    18661 cctgaatggt gtgagtggca gggtagccaa atgcgttggc aaacagagat tgtgtttttt
    18721 ctttcagact catttcttac actccggatt cgcgaaaatg gatatcgctg actgcgcgca
    18781 aacgctctgc tgcctgttct gcggtcaggt ctcgctgggt ctctgccagc atttcataac
    18841 caaccataaa tttacgtacg gtggcggagc gcaacagagg cggataaaag tgcgcgtgca
    18901 gctgccagtg ttgattctct tcgccattaa atggtgcgcc gtgccagccc atagagtagg
    18961 ggaaggagca ctggaagagg ttgtcataac gactggtcag ctttttcaac gccagcgcca
    19021 aatcgctgcg ctgggcgtcg gtcaaatcgg tgatccgcaa aacgtgggct ttgggcagca
    19081 gtagcgtttc gaacggccag gcagcccagt aaggcacaac ggctaaccag tgttcggttt
    19141 cgacaacggt acggctaccg tctgccagct cgcgctgaac ataatccacc agcattggtg
    19201 atttctgttc ggcaaaatat tctttttgca ggcggtcttc gcgctcagct tcgttaggca
    19261 ggaagctatt tgcccaaatc tgtccgtgcg gatgcgggtt agagcagccc atcgccgcac
    19321 ctttgttttc aaagacctgc acccacgggt acgtttttcc cagctctgcg gtttgctcct
    19381 gccaggtttt gacgatttcc gtcaatgccg caacgctcag ttctggcaac gttttactgt
    19441 gatccggtga aaagcagatc acccggctgg taccgcgcgc gctctggcaa cgcatcaaag
    19501 gatcgttgct ttctggcgca tctggcgtgt cagacatcaa ggccgcaaag tcattagtga
    19561 aaacgtaagt cccggtgtaa tcggggtttt tatcgcctgt cacccgcaca ttacctgcgc
    19621 agaggaagca atctggatcg tgcgcaggta acacctgttt ggctggcgtt tcctgcgccc
    19681 cctgccaggg gcgcttagcg cggtgcggtg aaaccagaat ccattgcccg gtgagcgggt
    19741 tgtagcggcg atgtggatga tcaacgggat taaattgcgt catggtcgtt ccttaatcgg
    19801 gatatccctg tggatggcgt gactgccagt gccaggtgtc ctgcgccatt tcatcgagtg
    19861 tgcgcgttac gcgccagttc agttcacggt cggctttgct ggcgtccgcc cagtaggccg
    19921 gaaggtcgcc ctcgcgacgc ggtgcaaaat gataattaac cggtttgccg caggctttgc
    19981 tgaaggcatt aaccacgtcc agcacgctgt tgcctacgcc agcgccgagg ttgtagatgt
    20041 gtacgcctgg cttgttcgcc agtttttcca tcgccacgac gtgaccgtcc gccagatcca
    20101 ttacgtggat gtaatcgcgt acgccagtac catcttcggt cggataatcg ttaccaaaaa
    20161 tcgccagcga gtcgcgacgg cctacagcaa cctgggcgat gtatggcatc aggttattcg
    20221 gaatgccttg cggatcttcg cccatatcgc ccgacggatg cgcgccaacc gggttgaagt
    20281 agcgcagcag ggcaatgctc cagtccggct gggctttttg cagatcggtg aggatctgtt
    20341 ccaccatcag cttgcttttg ccgtaagggc tttgtggtgt gccggtcggg aagctttcaa
    20401 cgtatggaat tttgggctga tcgccataaa cggtggcgga ggagctaaaa ataaagtttt
    20461 tgacgttagc ggcgcgcatg gcgctaatca ggcgcagagt gccgttgaca ttgttgtcgt
    20521 aatattccag cggtttttgt accgattcgc caacggcttt cagcccggcg aagtggatca
    20581 cggtgtcgat agcgtgatcg tgcagtatct cggtcatcaa cgcttcgtta cgaatatcgc
    20641 cttcaacaaa cgttggatgt ttgccgccta aacgctcgat aacaggcagt acgctgcgct
    20701 tactgttaca gaggttatca agaatgatga catcatgacc gttttgcagt aattgcacac
    20761 aggtatgact tccaatgtaa ccgctaccac cggtaaccag aactctcata attcgctcca
    20821 ttaggcttat ggtatgaaat aaccatagca taacaaagat gcgaaaagtg tgacatggaa
    20881 taaattagtg gaatcgttta cacaagaatt tagccgtttt ttatgcgcga ttaagtgatt
    20941 ataaaacaga gggtttatga atgattgcgc tttttatctg aaaaaagaag cggtttcatg
    21001 cctgcatgcg tcgaaccgtt ggccggagag ggcgcttagg ccgcctccgg caaggtcagc
    21061 actaccgact caatatattt ttgtcagcac atagcgatag agtccaccgt ccggcacgaa
    21121 ctcaaggcga tgggtaatac aggcaggcgc atcttcagcg tggtgcgaaa caaacaacaa
    21181 ttgcgtttca ccttcgctaa tcagcacatc aacaaaacgg cggataagct ggcgattgag
    21241 cggatcaagc ccctgtagtg gttcatcgag aataagcaac gtcggatgtt tcaccagtgc
    21301 gcggacgatc agcgccagac gctgctgtcc ccaggaaaga ctatggaacg gagcgtcagc
    21361 cgtacgttta tcaatgccga gaatatccag ccactgctgc accagttttt gctggcgatc
    21421 cgaaacggcc tgataaatgc caatcgaatc aaaatagcca gaaagaatca cattacgcac
    21481 agtagtactg acccggtaat ccagatgcaa actactgctg acgtaaccga tatgcttttt
    21541 gatatcccag atggtttcgc cgctgccgcg acgtcgtccg aaaagcgtca aatcgttgct
    21601 gtaaccttgc ggatgatcgc cagtaaccag gcttaataac gtcgattttc ctgcaccatt
    21661 tggcccgaca atttgccagt gttcgcctgg attcacctgc cagctaaggt tattaagaat
    21721 ggggcgatcg ttataagaaa ccacgccatt gttcagcaca atgcgcggtt cgttggcggg
    21781 taaggcgtga cgtgctgaag gttcatccgg ctccggcagt tgcacacctt caagctgttc
    21841 actatgcgcc agttgcgcga cgagtgcttg ttggagcagt tcctctttag cgccagtttc
    21901 cgctaacgtg caatccgcca gcacgccagc aaactggaca aactccggga tctcatcgaa
    21961 gcgattgagc accagtacca gagtaatacc ggactgatgt aacgaggcga gtcgctcagc
    22021 cagctgctga cgtgaggcaa catccaggcc atcgaacggc tcatcaagaa tcaacaagtc
    22081 aggctccgac atcagcgcct gacacagcag ggtttttcgc gtctcgccag tggaaaggta
    22141 tttaaagcgt cggtcgagga gggcggtaat accgaactgc tgcgccagtt gcatgcaacg
    22201 cggtgcatcc tttacttcat cctgaatgat ctcagccgta gtgcgtccgg tgtcatcttc
    22261 gccagggccg agcatatcgg tgttattccg ctgccattcg tcgctgacga gtttttgcaa
    22321 ttgctcgaag gagagacgag tgatgtggga aaactggctt tgccgttcac ctttcaaaag
    22381 cggaagttcc cccgccagcg cgcgggccag cgccgatttc ccgcttccat tcgaaccgac
    22441 aaacgcccaa ctatcacccg cgtttaacgt tagctgaggc aattgcagcg tttttgtgtc
    22501 gctaagacga aacgcgcctt gcaaaatttg caacgatgac attttatatc ccactttgtg
    22561 cagcgattac tgacagggat acgtgtttca taacaaattg tcaacacgct tagcacagcg
    22621 tggcgataat cacgctgtcg gcattaaagt aggccgtgac attctgtcct tgctgaagag
    22681 aagtcgcttc atttaccggc actgtggcgc acagtgtttg cccgtccggt agcgacatta
    22741 ttacttcgca ctgctctgcg ccgcgctcaa tatggctaat aatacccggt aattggttgt
    22801 cagcgttttg cgcgaccgct tcgtcctgag taatgcctac ccacggcgct tttagcaata
    22861 tcaacacttc tttgccttca tccagcccca gacgcgcgcc gctttgtgcg gtaattgcga
    22921 ctttcaggcg tgtttttccg tcagccagta agacatcaac atgctgttga acgtcatcat
    22981 gatcgcgggc ggtgatggta ccgaaccact ggttacgggc gctggtttgc agtgaaaaac
    23041 gtgagatcgc ggccaacagg ctgttcagcg gcagggcgtc atcgtcactt aacacatcaa
    23101 aggctttttg ctggatttgc gccagtaagt catagagctg aatcagtcgc tgaccatagc
    23161 gggtcagtac tgcgccgccg ccacctttac cgcctgttgc gcgctcgacc agaatatgct
    23221 cacttaactg attcatctcg ttaatggcat cccaggcgct tttatagctg ataccggcat
    23281 ctttcgctcc ctggctaatg gaaccggaaa gcgcaatgtg ttttagtagc gaaatgcggc
    23341 gcgggtcggc gaataatttt tgttggagct taagggtgag aaggatttcg gcctgcataa
    23401 caatgtcctg gcaaaagtct tattgtgacg gaaaacgaac gccacgcaaa gctgaccgca
    23461 caaaagggga gtgcttttct gtgcttagcg gttagaatag tctcatgact atatctggag
    23521 ttgaccatgt tagagttatt aaaaagtctg gtattcgccg taattatggt acctgtcgtg
    23581 atggccatca tcctgggtct gatttacggt cttggtgaag tattcaacat cttttctggt
    23641 gttggtaaaa aagaccagcc cggacaaaat cattgattcc ctgaatgccc gcttagtcgg
    23701 gcattttctt tttctcaact tcctgctttt cctgccgata ttttttctta tctacctcac
    23761 aaaggttagc aataactgct gggaaaattc cgagttagtc gttatattgt cgcctacata
    23821 acgttacatt aaggggttac caatggctcg taaatggttg aacttgtttg ccggggcggc
    23881 actctctttc gctgttgctg gcaatgcact ggcagatgaa gggaaaatca cggtgttcgc
    23941 cgccgcatca ctgactaacg caatgcagga cattgctacg cagtataaaa aagagaaagg
    24001 cgtggatgtg gtttcttctt tcgcttcgtc atctactctc gcccgtcaga ttgaagcggg
    24061 tgcccctgcg gatctgttta tttctgccga tcagaaatgg atggattatg cggttgataa
    24121 aaaagcgatc gatactgcta cgcgtcagac actgctcggc aatagcctgg tcgttgtagc
    24181 accgaaagcc agcgagcaga aagatttcac catcgacagc aaaactaact ggacttcgct
    24241 gctgaatggc ggtcgcctcg cggttggcga tccggaacat gtccctgctg gcatttatgc
    24301 aaaagaagca ctgcaaaaac tgggcgcatg ggatacgctc tccccgaaac tggctccggc
    24361 ggaagatgtt cgtggcgcgc tggcgttggt cgaacgcaat gaagcgccgt tgggcattgt
    24421 ttacggttct gacgcagttg ccagcaaagg ggtaaaagtg gttgccatct tcccggaaga
    24481 ttcacataaa aaagtggaat atccggttgc tgttgtggaa gggcataaca atgcgacagt
    24541 gaaagcattt tatgattatc tgaagggacc gcaggctgcg gaaatcttta aacgttacgg
    24601 atttacaacc aagtaatgat actgaccgat ccagaatggc aggcagtttt attaagcctg
    24661 aaagtttctt ccctggctgt gctgtttagc ctgccgtttg ggatcttttt tgcctggtta
    24721 ctggtgcgtt gcacgtttcc gggcaaagct ctgctcgaca gcgtactgca tctaccgctg
    24781 gtgttaccgc ccgtggtcgt cggttactta ttattagttt cgatgggacg gcgcggattt
    24841 atcggtgaac gtctgtatga ctggtttggt attaccttcg cctttagctg gcgcggcgcg
    24901 gttctcgctg ccgccgtcat gtcgtttccg ctgatggtgc gggcaattcg tctggcgctg
    24961 gaaggggttg atgtcaaact ggaacaggcc gcaagaacac tgggggccgg gcgctggcgc
    25021 gttttcttta ctatcacgtt accgctgacc ttaccgggaa ttattgttgg tacggtactg
    25081 gcttttgctc gttctctcgg tgagtttggt gcaaccatca cctttgtgtc gaacattcct
    25141 ggtgaaacgc gaaccattcc ttctgccatg tataccctga tccagacccc cggcggggaa
    25201 agtggagcgg cgagactgtg cattatttct attgcgctgg cgatgatctc cctgttgatt
    25261 tcagaatggc tggccagaat cagccgtgaa cgggcggggc gctaatcatg ctggaactga
    25321 atttttccca gacgttgggc aaccattgcc tgactattaa tgaaacgctg cccgccaatg
    25381 gcatcactgc tatctttggc gtctccggtg ccggaaaaac ttcgctgatt aacgccatca
    25441 gtggactgac gcgcccgcaa aaagggcgga ttgtcctcaa tgggcgggta ctaaatgatg
    25501 ccgaaaaagg tatctgcctg acgccggaaa agcgtcgcgt tggctatgtt tttcaggatg
    25561 cgcggctgtt cccgcattac aaagtgcgtg gcaatctgcg ctacggcatg tcgaaaagta
    25621 tggtcgatca gttcgataag ctggtggcgc ttttaggcat tgaaccgttg cttgaccgtt
    25681 taccaggcag cctgtccgga ggcgaaaaac agcgcgtggc gattggtcgg gctttgctga
    25741 cagcaccgga attgctgttg ctggatgaac cgctggcgtc actggatatt ccgcgtaaac
    25801 gcgaactgtt gccttatctg caacggctga cacgggaaat caacattccg atgttgtatg
    25861 tcagccattc gctggatgag atcctccatc tggcagacag agtgatggta ctggaaaacg
    25921 gtcaggtgaa agcctttggc gcgctggagg aagtgtgggg cagtagcgtg atgaatccgt
    25981 ggctgccgaa agagcaacaa agtagcattc tgaaagtgac ggtgcttgag catcatccgc
    26041 attacgcgat gaccgcgctg gcgctgggcg atcagcattt gtgggtcaat aagctggacg
    26101 aaccgctgca agctgcgcta cgtatccgca ttcaggcttc cgatgtttct ctggttttac
    26161 aaccgccgca gcaaaccagc attcgtaacg tattgcgggc aaaagttgtt aatagttatg
    26221 acgacaacgg ccaggtggaa gtggaactgg aagtcggcgg taaaacgctg tgggcgcgta
    26281 tcagcccgtg ggccagggat gaactggcga tcaaacctgg cctgtggctg tacgcgcaaa
    26341 ttaaaagtgt gtcgataacc gcctgattaa atcaggtggc tataaatgaa ctgggcaatg
    26401 ctgtcggtgg tgttatcacc aatcacaatg ttggcgcgcg cttttaccgc gtcatcggcg
    26461 ttgcccatcg ccacgcctgt accagcggct tccagcatac tgatatcatt aaagttatcg
    26521 ccgaatgcca cgacgttttc catcgaccaa ccttgcgcct caacccattt cgtcaaacgt
    26581 ttacctttgc tgttgccgcc gcgtgcaata tcaacctgat cgtgccagga ccattcacac
    26641 tccagtccca gttcatgttc gacatgctta ccaaaatgct gcaattgcgg caggtcatcg
    26701 tgcgtcaggg cgaacttcca tacggcgtta acttgttgcg ccgtttcagc cagagaagcg
    26761 acttgtgtga aagtcggacg ctgttccggc ggcagggttt gcgcccagtt agacgtgcga
    26821 atgacatgcc cggtcgggtg ctcatagacc attgcatcat ccacatacat cagaccgtga
    26881 atgtggtgtt cattcagcat ctcaatgagt tgcagggctt tattaacggg cattgggtcc
    26941 gcttccagca cggtttttgc atgataatca tacaaatagg tgccattaca gcaaatagca
    27001 ggtgtatcca gcgccagcgc ctgataaaaa ggatgaatag cgacgtgatg gcgacctgtg
    27061 acgatgatta attgatagcc tgcttcgcga gcgcgggcca gggcttctat cgatgaagga
    27121 agcagggtct ttttcggggt caataaggtg ccgtctaagt cgagagcaat cacgcgtgtg
    27181 gtcatggcgt attccagatt aaggttaaga attttcgtct gcgcgaatgg tacaccgata
    27241 ccactatcgg acaaaattct gcgttttaat tcagcattca ccgccaaaag cgactaattt
    27301 tagctgttac agtcagttgc taaatgcaaa ggagcattca tgaagcaaac agtttatatc
    27361 gccagccctg agagccagca aattcacgtc tggaacctga atcatgaagg cgcactgacg
    27421 ctgacacagg ttgtcgatgt gccggggcag gtgcagccga tggtggtcag cccggacaaa
    27481 cgttatctct atgttggtgt tcgccctgag tttcgcgtcc tggcgtatag tatcgccccg
    27541 gacgatggcg cactgacctt tgccgcagag tctgcgctgc cgggtagtcc gacgcatatt
    27601 tccaccgatc accaggggca gtttgtcttt gtaggttctt acaatgcggg taacgtgagc
    27661 gtaacgcgtc tggaagatgg cctgccagtg ggcgtcgtcg atgtggtcga ggggctggac
    27721 ggttgccatt ccgccaatat ctcaccggac aaccgtacgc tgtgggttcc ggcattaaag
    27781 caggatcgca tttgcctgtt tacggtcagc gatgatggtc atctcgtggc gcaggaccct
    27841 gcggaagtga ccaccgttga aggggccggc ccgcgtcata tggtattcca tccaaacgaa
    27901 caatatgcgt attgcgtcaa tgagttaaac agctcagtgg atgtctggga actgaaagat
    27961 ccgcacggta atatcgaatg tgtccagacg ctggatatga tgccggaaaa cttctccgac
    28021 acccgttggg cggctgatat tcatatcacc ccggatggtc gccatttata cgcctgcgac
    28081 cgtaccgcca gcctgattac cgttttcagc gtttcggaag atggcagcgt gttgagtaaa
    28141 gaaggcttcc agccaacgga aacccagccg cgcggcttca atgttgatca cagcggcaag
    28201 tatctgattg ccgccgggca aaaatctcac cacatctcgg tatacgaaat tgttggcgag
    28261 cagggactac tgcatgaaaa aggccgttat gcggtcgggc agggaccaat gtgggtggtg
    28321 gttaacgcac actaaccgct gatttaaccg gcgcagtctc tcctgcgccg gtgtattaac
    28381 ctatctcctg taacgcatgt ctctggcgtt cgacgatatt ggtccacaaa ttgtctttat
    28441 cgtcagtcca caaattaatc agcaaggcaa aaaagcgttc tgctgccgga gaaagcacgg
    28501 catctttgcg gcgaataatc cccaatgtcc gacgtataac gggttccacc agtggaatac
    28561 caataatcga agaatagggc gcatggggca ttgccagacc aggaagcgcc gaaatcccca
    28621 gtcccgcctc taccagtcct aatgacgtcg aaagatgacg cacttcgtaa aaccagtcca
    28681 gcttccaggg cttgtcggct agctgttgtt ctatcaacaa tcggttgccg ctggaggaac
    28741 gtacgccaat cattttgtaa cccactaact cctgccattc tacaagctgc tttttggcca
    28801 atggatggtc acgccgacag gccagcacga atggctcgtt gaccagtggg gtaaaatcaa
    28861 tggatgaatt tgtgacgttg ttcatgttta tgccaaagtc tgattcgtta cacagcaccg
    28921 attccatgca attattcgtg ccttgttcca gaatccgcac tttaatattg ggatacagct
    28981 cattaaattt accgattgcc agcggtaaaa aataaaatac tgcggtcgga atacacgcta
    29041 acgtcaccat accacgatga taagcattca tatcacgaat attaaaaagc gtttcatcga
    29101 atttctttat taattcccgc gcctccggga gcaacctttt tcctgctttt gttaacgtaa
    29161 ccttgcgagt tgtacgctca aatagttgaa tatgtaaatc ttcctccatc ttttttattc
    29221 ttcgcgttaa tgcaggttgc gtaatattga gtaatttagc agcattgtta aatgaactgg
    29281 actctgccag tatgacaaat gccttcatac ttgataattc atgcttcatt attactccgg
    29341 aaaatggaag cgacgatttt gggtggctgg tcgttaaaaa ttttaactgc atttagccaa
    29401 cttaaattaa tgaaaaaatg ttattaatcg ttgagctaaa gtcattagag atgctttgtc
    29461 cttaatgtaa ccatatcgca ataagttatg tttttaaatt gagggcatta ttatgaaaaa
    29521 aataccctgc gtgatgatgc gaggtggaac ctcgaggggc gcgttcctgt tagcggaaca
    29581 tttacccgaa gatcaaacgc agcgcgataa aatattgatg gcaattatgg gttccggtaa
    29641 cgatctggaa attgacggta ttggcggcgg taatccactg accagtaaag tcgccattat
    29701 tagccgttcc agcgatccgc gtgctgatgt tgattatctg tttgctcaag ttatcgtcca
    29761 tgagcaacgt gtcgatacca cgcctaactg cggcaatatg ctttctggcg ttggggcatt
    29821 cgccattgaa aatggtttga ttgcagcgac atcaccggtc acccgtgtgc gtatccgcaa
    29881 cgtcaatacg ggtacgttca tcgaagctga tgtgcaaacg ccaaatggtg ttgtcgagta
    29941 cgagggtagt gccagaattg acggcgtacc gggtactgcc gcaccggttg cgctcacttt
    30001 cctgaatgcc gctggaacca aaaccggaaa agttttcccg actgataatc agattgatta
    30061 ttttgacgat gtcccggtga cctgtatcga tatggcgatg ccagtcgtca ttattccggc
    30121 tgaatatctg ggaaaaacag gttatgaatt accggcggaa ctggatgccg acaaagcatt
    30181 attagcccgc attgaatcta tccgtctaca agcgggtaaa gcaatgggct taggtgatgt
    30241 cagtaatatg gttatcccta aacctgtgct tatttctcca gcgcagaaag gcggggcaat
    30301 taatgtgcgt tattttatgc cgcattcttg ccatcgcgcg ctggcgataa ccggtgctat
    30361 tgctatttcc agtagttgtg cattggaagg caccgtcacc cgacaaatcg tcccttctgt
    30421 aggatacggc aatatcaata ttgaacaccc cagtggtgcg ctcgacgttc atttaagtaa
    30481 tgaaggtcag gatgccacga cgttacgcgc atctgttatt cggacgacca gaaaaatatt
    30541 ttccggtgaa gtttatcttc cctgaaaaaa ttcgttgtca agataaggat aatcaataaa
    30601 ggacttctgt atgagtcata cagaaagaac aggattttaa atgaataaga aatcgttatg
    30661 gaagctaatt ctgatattag cgatcccatg tattattggt tttatgccag ctccggcagg
    30721 attaagcgaa ctggcgtggg tgctttttgg tatttacctg gcggccattg tggggctggt
    30781 tatcaagcct ttcccggaac ctgtcgtact gttaattgcc gttgctgcct ccatggtggt
    30841 ggtcggtaac ttatccgacg gtgcgtttaa gaccaccgcc gtattaagcg gttactcttc
    30901 aggtactacc tggctggtgt tctcggcgtt taccttaagc gccgcatttg tgaccaccgg
    30961 tttaggtaaa cgtattgcct atctgctgat tggtaaaatc ggtaacacca cgctgggtct
    31021 gggttacgtt acggtattcc tcgatctggt actggctccg gcaacaccgt ctaacaccgc
    31081 gcgtgcgggc ggtattgtgt taccgatcat caacagcgtg gcggtggctt tggggtccga
    31141 accggaaaaa agtccgcgtc gtgtcggaca ttacctgatg atgtccattt acatggtcac
    31201 caaaaccacc agctatatgt tctttaccgc aatggcgggg aacattctgg cgctgaaaat
    31261 gatcaacgac attctgcacc tgcaaattag ctggggtgga tgggcactgg ccgccggatt
    31321 gccgggcatc attatgctgc tggtcacccc acaggtgatt tataccatgt atccgccaga
    31381 aattaagaag gtggataata aaaccatcgc taaagcgggc cttgccgaac tgggaccgat
    31441 gaaaatccgc gaaaaaatgc tgctcggtgt ctttgtgctg gcgctactgg gctggatttt
    31501 cagtaagtct ctgggggttg atgaatccac tgtggcaatc gttgttatgg cgaccatgct
    31561 gctgctgggt atcgttacct gggaagacgt ggttaaaaat aaaggcggct ggaatacctt
    31621 aatctggtac ggcggtatta tcggattaag ctccttatta tcgaaagtta aattcttcga
    31681 atggttagct gaagtcttta aaaataacct ggcatttgat ggtcacggta acgttgcttt
    31741 cttcgttatt attttcctca gcattatcgt gcgttatttc ttcgcttccg gtagtgccta
    31801 tatcgttgct atgttaccgg tatttgccat gctggcgaac gtctccggcg caccgttaat
    31861 gttaaccgcg ctggcactgt tgttctccaa ctcctatggc ggcatggtta ctcactatgg
    31921 cggcgcggca ggtccggtca tctttggcgt gggttataac gatattaaat cctggtggtt
    31981 ggtcggtgcg gtactgacga tattaacctt cctggtgcat atcaccctcg gcgtgtggtg
    32041 gtggaatatg ctgatcggct ggaacatgct gtaaatattc tcgtcatact tcaagttgca
    32101 tgtgctgcgt ctgcgttcgc tcaccccagt cacttactta tgtaagctcc tggggattca
    32161 ctcgcttgtc gccttcctgc aactcgaatt atttagagta tatccattta ttatctttct
    32221 gcgcacttca cggtgcgcag atatctggag catttgatga tcaagttatc tgaaaaaggc
    32281 gtgtttctcg ccagtaataa cgaaataatt gccgaagaac atttcaccgg cgaaattaaa
    32341 aaagaagaag ccaaaaaagg cactattgcc tggtctattc tctcttcgca taatacgtcc
    32401 ggaaatatgg ataaacttaa aattaagttt gattcattag cctctcacga tattaccttt
    32461 gttggtattg tacagaccgc taaagcgtcc ggtatggaac gtttcccgct gccgtatgtg
    32521 ctgaccaact gccataactc actctgcgcc gtcggcggca ctattaacgg tgatgaccat
    32581 gtttttggtt tatcggcggc ccagcgttat ggcggtattt ttgtgcctcc gcatattgcg
    32641 gtcatccatc aatatatgcg tgagatgatg gcaggcggcg gcaaaatgat cctcgggtca
    32701 gacagccaca cccgttacgg tgcattaggg acaatggcag tcggtgaggg cggcggtgag
    32761 ttggtaaaac agctgcttaa tgacacctgg gatatcgact atccgggcgt ggttgcggtg
    32821 catctgaccg gaaaacctgc gccgtatgtg gggccacagg atgtggcgct ggctatcatt
    32881 ggcgcggtgt tcaaaaacgg ttacgtcaaa aacaaagtca tggagttcgt tggaccgggc
    32941 gttagcgcgc tctctaccga tttccgtaac agcgttgacg tgatgaccac ggaaaccacc
    33001 tgtttaagct ccgtctggca aactgatgaa gaagtgcata actggctggc gctgcacggt
    33061 cgcggccagg attactgcca gcttaaccct caaccgatgg cgtactacga tggctgcatc
    33121 agcgttgatt taagcgccat caaaccaatg attgcgctgc cgttccaccc gagcaacgtg
    33181 tataaaatcg acacactgaa ccagaacctg accgacattc tgcgtgagat tgaaattgag
    33241 tccgaacgcg tggcgcacgg taaagccaaa ctctcgctgc tggataaagt ggaaaatggt
    33301 cgcctgaaag tgcagcaggg gattatcgcg ggctgttctg gcggtaacta cgaaaacgtc
    33361 atcgcggcgg cgaatgcact gcgcggtcaa tcctgtggca atgacacctt ctcgctggca
    33421 gtttacccgt catcacagcc ggtgtttatg gatctcgcca aaaaaggtgt ggtagcagat
    33481 ttgattggcg caggcgcaat catcagaacc gcgttctgcg gcccatgctt tggcgcgggc
    33541 tatacgccaa tcaacaacgg tttgagtatt cgccacacca cgcgtaactt cccgaaccgc
    33601 gaaggctcta agccagctaa tgggcagatg tcagcggtgg cgttgatgga cgctcgttct
    33661 atcgctgcga ctgcggcaaa cggtggctat ttaacctctg ccagcgaact tgattgctgg
    33721 gacaacgtgc cggagtacgc cttcgatgta acgccgtata aaaaccgtgt ttatcagggc
    33781 tttgtgaaag gggcaactca gcaaccgctg atttacggac cgaacattaa agactggccg
    33841 gaattgggtg cgctgactga caatatcgtc ctgaaagtgt gctcgaagat cctcgacgaa
    33901 gtgaccacca ccgacgaact gattccttcc ggtgaaacct cttcttatcg ttcaaatccg
    33961 attggtctgg cggagtttac cctgtcacgc cgcgatcccg gttatgttgg cagaagtaaa
    34021 gcgactgctg agctggaaaa tcagcgtctg gcggggaatg tcagcgagct gacagaggtg
    34081 tttgcgcgca ttaagcagat tgctggtcag gagcatattg atccgctgca aactgaaatt
    34141 ggcagcatgg tatatgcggt gaaaccaggc gatggttctg cgcgtgaaca ggcggcgagc
    34201 tgccagcgtg tgattggcgg tctggcgaat attgccgaag agtacgcgac taaacgctac
    34261 cgttctaacg tcatcaactg ggggatgtta ccgctgcaga tggcggaagt gccaaccttt
    34321 gaagtggggg attacattta catccctggc attaaagcgg cgctggataa tccgggtacg
    34381 acgtttaaag gttatgtgat ccatgaagat gcgccggtaa cggaaattac gctctatatg
    34441 gaaagtctca ctgctgaaga gcgcgagatt atcaaggcgg gtagtttgat taacttcaat
    34501 aaaaaccgtc agatgtaaaa agcgccatgt gaatgtaggt cgcattcggc acttattgtc
    34561 ggatgcgatg cttgcgcatc ttatccgacc tacgaatcgc atcgaatctg taggccagat
    34621 aaggcatttt cgcagcatcc ggcacttatt gtcggatgcg atgcttgcgc atcttatccg
    34681 acctacaaat cgcatcgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcaaata
    34741 gttaattgct cttacttctt cgcctctgca accactttac tacccacgcc gcggttattg
    34801 tattcccaca tgcggttgta gttagtgtca ttcagattgc gctgtatttc gtcgttatca
    34861 tctacgctgc cggtattacc cgcaaacgga cgattagaga tcaccgcatc ggcccacggt
    34921 ttagccgtgt taaaaccttc gttgatggcg ctatcacgga tcaccacctg accgttggta
    34981 ttggcatcaa catccagcga gcggcccagt tgcgccacac catcaccgaa agcattgaaa
    35041 cggctgttta cggcgaggaa accgtagtaa atgttggaca gcgtagccgg tgcaaacaca
    35101 tacgcttctt gctgagtacg tgagttcacc acgcggaatt cggtgttatc gaacaccact
    35161 gcgccgcgac cagaaacgat atccacatcc ccttcaatgt agctgttggt caccagcgta
    35221 cgcggctgac gattcgtttc cagacggttc tgcacaccgc tgttggtgac aaagaaggtg
    35281 ttctgacgac cgagaatgtt aacgttgtta atctgtacct ggtcaccatc agtacgcagt
    35341 gccaccgccg gatggttacc tgcatctacg ctatcgccca gcgtgttttc gatggtcaga
    35401 ttttgcagtt gcaggccatt gttttgtgac cagaagaccg cagagcagag aacaccgata
    35461 ctgtcgctgc gtttgctctg gcagctatcg tacatatacc acgctggttt acctggcata
    35521 tatttgccgc gcgggttgac gtcgtgacgc cagtcggcag ggctcatgcc accatcaagg
    35581 gaaagcccaa tcttcacatc aatcggtttt tcacctgtac cgtacagagt aattccaccc
    35641 ggagcggcag ggacatatac cgttccctga tactcaccag gcatcacggc aatatactgg
    35701 cgcttgttgg tacgcttgat aattgccgca tctaccgccg cctgaatcgt ggtatgcgtt
    35761 acaccttgag tgcccgccgg gccgacaaca aagtcaggtt gcgcaggcag ggtaatcggg
    35821 gaaggattcc acgctgctgc acctggtgtc agggatgcaa aatagtgttg agcatcgaaa
    35881 ttctgcgctt cttttgccga cagaatcggg cgagaagagg taccaggcgc ggtttgatca
    35941 gaaggacgtt gatcgggcgg ggttgagcta caggcggtca gcgtcacgcc aaaagccaat
    36001 gccagcgcca gacgggaaac tgaaaatgtg ttcacaggtt gctccgggct atgaaataga
    36061 aaaatgaatc cgttgaagcc tgctttttta tactaacttg agcgaaacgg gaaggtaaaa
    36121 agacaaaaag ttgtttttaa tacctttaag tgataccaga tggcattgcg ccatctggca
    36181 gagtgattaa ctaaacatcg cagtaatcga ggcgcttgcc agagagtgga aatgaacgtt
    36241 aaacccgacc atcgcgccgc tggcaccttc atcgacatca atacgttcta tatccagcgc
    36301 gtgaacggta aaaatgtagc gatgagtttc gcctttcggc ggtgctgcgc catcgtaccc
    36361 ggttttacca aagtcggtac gcgtctgcaa aacgccgtct ggcattgcta ccagaccaga
    36421 gccaaaccct tgcggtaata cgcgggtatc agcgggtaag ttaacaacta cccagtgcca
    36481 ccagccggag ccggttggcg catccgggtc gtagcaggtg acaacaaaac ttttcgttcc
    36541 cgcaggaaca tcatcccacg ccagatgcgg tgaaatatta tcgccatcgt aacccatgcc
    36601 gttaaagaca tgacgatgcg gcaatttatc gccatcgcgc agatcgttac tgatgagttt
    36661 catgaaccct cctttcttgt ttgcagaaag tgtagccaga aaccctcacg cggacttccc
    36721 gttattggca aaaaaatgtt tcatcctgta ccgcgcggtt aaccgctgcg gtcagacgct
    36781 gcaactgttg cgggagaata atatagggcg gcatcaggta aatcagtttg ccaaaaggcc
    36841 ggatccagac accctgttcg acaaagaatt tttgcagcgc cgccatattc accggacgag
    36901 tggtttcgac cacaccgatt gcccccagta cgcgcacatc ggcaaccatt tcggcatcac
    36961 gggctggtgc cagttgctcg cgcagctgca cttcaatagc cgccacctgc tgctgccatt
    37021 cgccggattc gataatcgcc aggctggcgt ttgctgccgc gcaggccagc ggattgccca
    37081 taaaagttgg cccatgcata aagcagccgg cttcgccgtt actgatggtt tctgcaacct
    37141 cgcgcgtggt aagtgtggcg gaaagggtca ttgtgccgcc ggttaaggct ttaccgaggc
    37201 acaaaatgtc cggcgcgatt tctgcatatt cacaggcaaa cagtttgccg gtacgaccaa
    37261 atccggtggc gatctcgtcg gcaatcagca agataccttc gcgatcgcac attttgcgga
    37321 ttcgttttaa ccattccgga tggtacatgc gcatcccgcc tgcgccctgg acaatcggct
    37381 caatgatcac cgccgcgatt tcatgacgat gcgccgccat caggcgggca aagcccacca
    37441 tatcgcgctc atcccattcg ccatccatgc ggctttgcgg ggcgggagca aacaggtttt
    37501 ctggcaggta gcctttccac agactgtgca ttgagttatc cggatcgcac accgacatcg
    37561 cgccaaaggt atcgccatga taaccattgc ggaaggtcag aaaacgctgg cgcgcttcgc
    37621 ctttggcttg ccagtactgc aacgccattt tcatcgccac ttccaccgct acggaaccgg
    37681 agtccgcgag aaaaacgcac tccagcggtt gcggcgtcat cgccaccagt ttgcggcaca
    37741 gctcaatggc tggcgcatgg gtgataccgc caaacatcac atgcgacatg gcatcaattt
    37801 gcgacttcat cgccgcatta agctgcgggt gattgtagcc gtggatcgcc gcccaccagg
    37861 acgacatacc gtcaaccagg cgtttgccgt cagacaaaat cagctcgcaa ccttcggcgc
    37921 tcaccaccgg ataaaccggc agaggggagg tcatggatgt gtatgggtgc cagatatggc
    37981 gttggtcaaa ggcaagatcg tccgttgtca taatcgactt gtaaaccaaa ttgaaaagat
    38041 ttaggtttac aagtctacac cgaattaaca acaaaaaaca cgttttggag aagccccatg
    38101 gctcaccgcc cacgctggac attgtcgcaa gtcacagaat tatttgaaaa accgttgctg
    38161 gatctgctgt ttgaagcgca gcaggtgcat cgccagcatt tcgatcctcg tcaggtgcag
    38221 gtcagcacgt tgctgtcgat taagaccgga gcttgtccgg aagattgcaa atactgcccg
    38281 caaagctcgc gctacaaaac cgggctggaa gccgagcggt tgatggaagt tgaacaggtg
    38341 ctggagtcgg cgcgcaaagc gaaagcggca ggatcgacgc gcttctgtat gggcgcggcg
    38401 tggaagaatc cccacgaacg cgatatgccg tacctggaac aaatggtgca gggggtaaaa
    38461 gcgatggggc tggaggcgtg tatgacgctg ggcacgttga gtgaatctca ggcgcagcgc
    38521 ctcgcgaacg ccgggctgga ttactacaac cacaacctgg acacctcgcc ggagttttac
    38581 ggcaatatca tcaccacacg cacttatcag gaacgcctcg atacgctgga aaaagtgcgc
    38641 gatgccggga tcaaggtctg ttctggcggc attgtgggct taggcgaaac ggtaaaagat
    38701 cgcgccggat tattgctgca actggcaaac ctgccgacgc cgccggaaag cgtgccaatc
    38761 aacatgctgg tgaaggtgaa aggcacgccg cttgccgata acgatgatgt cgatgccttt
    38821 gattttattc gcaccattgc ggtcgcgcgg atcatgatgc caacctctta cgtgcgcctt
    38881 tctgccggac gcgagcagat gaacgaacag actcaggcga tgtgctttat ggcaggcgca
    38941 aactcgattt tctacggttg caaactgctg accacgccga atccggaaga agataaagac
    39001 ctgcaactgt tccgcaaact ggggctaaat ccgcagcaaa ctgccgtgct ggcaggggat
    39061 aacgaacaac agcaacgtct tgaacaggcg ctgatgaccc cggacaccga cgaatattac
    39121 aacgcggcag cattatgagc tggcaggaga aaatcaacgc ggcgctcgat gcgcggcgtg
    39181 ctgccgatgc cctgcgtcgc cgttatccgg tggcgcaagg agccggacgc tggctggtgg
    39241 cggatgatcg ccagtatctg aacttttcca gtaacgatta tttaggttta agccatcatc
    39301 cgcaaattat ccgtgcctgg cagcaggggg cggagcaatt tggcatcggt agcggcggct
    39361 ccggtcacgt cagcggttat agcgtggtgc atcaggcact ggaagaagag ctggccgagt
    39421 ggcttggcta ttcgcgggca ctgctgttta tctctggttt cgccgctaat caggcagtta
    39481 ttgccgcgat gatggcgaaa gaggaccgta ttgctgccga ccggcttagc catgcctcat
    39541 tgctggaagc tgccagttta agcccgtcgc agcttcgccg ttttgctcat aacgatgtca
    39601 ctcatttggc gcgattgctt gcttccccct gtccggggca gcaaatggtg gtgacagaag
    39661 gcgtgttcag catggacggc gatagtgcgc cactggcgga aatccagcag gtaacgcaac
    39721 agcacaatgg ctggttgatg gtcgatgatg cccacggcac gggcgttatc ggggagcagg
    39781 ggcgcggcag ctgctggctg caaaaggtaa aaccagaatt gctggtagtg acttttggca
    39841 aaggatttgg cgtcagcggg gcagcggtgc tttgctccag tacggtggcg gattatctgc
    39901 tgcaattcgc ccgccacctt atctacagca ccagtatgcc gcccgctcag gcgcaggcat
    39961 tacgtgcgtc gctggcggtc attcgcagtg atgagggtga tgcacggcgc gaaaaactgg
    40021 cggcactcat tacgcgtttt cgtgccggag tacaggattt gccgtttacg cttgctgatt
    40081 catgcagcgc catccagcca ttgattgtcg gtgataacag ccgtgcgtta caactggcag
    40141 aaaaactgcg ccagcaaggc tgctgggtca cggcgattcg cccgccaacc gtacccgctg
    40201 gtactgcgcg actgcgctta acgctaaccg ctgcgcatga aatgcaggat atcgaccgtc
    40261 tgctggaggt gctgcatggc aacggttaat aaacaagcca ttgcagcggc atttggtcgg
    40321 gcagccgcac actatgagca acatgcagat ctacagcgcc agagtgctga cgccttactg
    40381 gcaatgcttc cacagcgtaa atacacccac gtactggacg cgggttgtgg acctggctgg
    40441 atgagccgcc actggcggga acgtcacgcg caggtgacgg ccttagatct ctcgccgcca
    40501 atgcttgttc aggcacgcca gaaggatgcc gcagaccatt atctggcggg agatatcgaa
    40561 tccctgccgt tagcgactgc gacgttcgat cttgcatgga gcaatctcgc agtgcagtgg
    40621 tgcggtaatt tatccacggc actccgcgag ctgtatcggg tggtgcgccc caaaggcgtg
    40681 gtcgcgttta ccacgctggt gcagggatcg ttacccgaac tgcatcaggc gtggcaggcg
    40741 gtggacgagc gtccgcatgc taatcgcttt ttaccgccag atgaaatcga acagtcgctg
    40801 aacggcgtgc attatcaaca tcatattcag cccatcacgc tgtggtttga tgatgcgctc
    40861 agtgccatgc gttcgctgaa aggcatcggt gctacgcatc ttcatgaagg gcgcgacccg
    40921 cgaatattaa cgcgttcgca attgcagcga ttgcaactgg cctggccgca acaacagggg
    40981 cgatatcctc tgacgtatca tctttttttg ggagtgattg ctcgtgagta aacgttattt
    41041 tgtcaccgga acggataccg aagtggggaa aaccgtcgcc agttgtgcac ttttacaagc
    41101 cgcgaaggca gtaggttatc ggacggcagg ttataaaccg gtcgcctctg gcagcgaaaa
    41161 gaccccggaa ggcttacgta atagtgacgc gctggcgtta cagcacaaca gcagcctgca
    41221 gctggattac gcaacggtaa atccttacac cttcgcagaa cccacctcgc cgcacatcat
    41281 cagcgcgcaa gagggcaggc cgatagaatc attggtaatg agcgccggat tacgcgcgct
    41341 tgaacaacag gctgactggg tgttagtgga aggtgctggc ggctggttta cgccgctttc
    41401 tgacactttc acttttgcag attgggtaac acaggaacaa ctgccggtga tactggtagt
    41461 tggtgtgaaa ctcggctgta ttaatcacgc gatgttgact gcgcaggcaa tacaacacgc
    41521 cggactgact ctggcgggtt gggtggcgaa cgatgttacg cctccgggaa aacgtcacgc
    41581 tgaatatatg accacgctca cccgcatgat tcccgcgccg ctgctgggag agatcccctg
    41641 gcttgcagaa aatccagaaa atgcggcaac cggaaagtac ataaaccttg ccttgttgta
    41701 gctatcctgt atttggttaa attgcgagcg agatcgcgtc ttcgattgac tgcaatttaa
    41761 ccaattaaat tctaaaataa tcacgaaaaa aattttactt ccgcctcatg cggcgaatgt
    41821 gggaattgcc caggcggcgg gg
//
LOCUS       DAJOWB010000026        41533 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJOWB010000026 DAJOWB010000000
VERSION     DAJOWB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.026"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            11..517
                     /locus_tag="OHO85_001612"
     CDS             11..517
                     /locus_tag="OHO85_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCQ2670995.1"
                     /translation="MTCISQPKKTTLRDKAMVNYAFDYLRSPGSLPFTTAATELSAIH
                     GHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKGLGVKP
                     DTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQKWLKLAAKQHYKNAER
                     LLAGLPAH"
     gene            complement(527..1954)
                     /locus_tag="OHO85_001613"
     CDS             complement(527..1954)
                     /locus_tag="OHO85_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCQ2670996.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYAQSWREAEPDNEDAPYYEYAQRVYCGEGESLLAELCD
                     HWREYPSTQADALMLQWCRQHRVDYYPLLVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQACQPT
                     EESLMPIVSRLDAENRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1951..2658)
                     /locus_tag="OHO85_001614"
     CDS             complement(1951..2658)
                     /locus_tag="OHO85_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCQ2670997.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            2822..3799
                     /locus_tag="OHO85_001615"
     CDS             2822..3799
                     /locus_tag="OHO85_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCQ2670998.1"
                     /translation="MIFTSTCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3869..4351)
                     /locus_tag="OHO85_001616"
     CDS             complement(3869..4351)
                     /locus_tag="OHO85_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCQ2670999.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4586..7168
                     /gene="leuS"
                     /locus_tag="OHO85_001617"
     CDS             4586..7168
                     /gene="leuS"
                     /locus_tag="OHO85_001617"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCQ2671000.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7183..7764
                     /gene="lptE"
                     /locus_tag="OHO85_001618"
     CDS             7183..7764
                     /gene="lptE"
                     /locus_tag="OHO85_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCQ2671001.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7764..8795
                     /gene="holA"
                     /locus_tag="OHO85_001619"
     CDS             7764..8795
                     /gene="holA"
                     /locus_tag="OHO85_001619"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCQ2671002.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8797..9438
                     /gene="nadD"
                     /locus_tag="OHO85_001620"
     CDS             8797..9438
                     /gene="nadD"
                     /locus_tag="OHO85_001620"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCQ2671003.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9462..10073
                     /locus_tag="OHO85_001621"
     CDS             9462..10073
                     /locus_tag="OHO85_001621"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCQ2671004.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSTIDINQKFATLRVLNSRAIGVENA"
     gene            10333..10650
                     /gene="rsfS"
                     /locus_tag="OHO85_001622"
                     /gene_synonym="rsfA"
     CDS             10333..10650
                     /gene="rsfS"
                     /locus_tag="OHO85_001622"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCQ2671005.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10654..11121
                     /gene="rlmH"
                     /locus_tag="OHO85_001623"
     CDS             10654..11121
                     /gene="rlmH"
                     /locus_tag="OHO85_001623"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCQ2671006.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11152..13053
                     /gene="mrdA"
                     /locus_tag="OHO85_001624"
     CDS             11152..13053
                     /gene="mrdA"
                     /locus_tag="OHO85_001624"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCQ2671007.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFANAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13056..14168
                     /gene="mrdB"
                     /locus_tag="OHO85_001625"
                     /gene_synonym="rodA"
     CDS             13056..14168
                     /gene="mrdB"
                     /locus_tag="OHO85_001625"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCQ2671008.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14179..15267
                     /gene="rlpA"
                     /locus_tag="OHO85_001626"
     CDS             14179..15267
                     /gene="rlpA"
                     /locus_tag="OHO85_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCQ2671009.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15407..16618
                     /gene="dacA"
                     /locus_tag="OHO85_001627"
     CDS             15407..16618
                     /gene="dacA"
                     /locus_tag="OHO85_001627"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCQ2671010.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16729..16992
                     /locus_tag="OHO85_001628"
     CDS             16729..16992
                     /locus_tag="OHO85_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCQ2671011.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17093..17734
                     /gene="lipB"
                     /locus_tag="OHO85_001629"
     CDS             17093..17734
                     /gene="lipB"
                     /locus_tag="OHO85_001629"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCQ2671012.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17993..18946
                     /locus_tag="OHO85_001630"
     CDS             17993..18946
                     /locus_tag="OHO85_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCQ2671013.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19155..20120
                     /gene="lipA"
                     /locus_tag="OHO85_001631"
     CDS             19155..20120
                     /gene="lipA"
                     /locus_tag="OHO85_001631"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCQ2671014.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20221..20424)
                     /gene="tatE"
                     /locus_tag="OHO85_001632"
     CDS             complement(20221..20424)
                     /gene="tatE"
                     /locus_tag="OHO85_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCQ2671015.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20553..21341)
                     /locus_tag="OHO85_001633"
     CDS             complement(20553..21341)
                     /locus_tag="OHO85_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCQ2671016.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21434..21817
                     /gene="crcB"
                     /locus_tag="OHO85_001634"
     CDS             21434..21817
                     /gene="crcB"
                     /locus_tag="OHO85_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCQ2671017.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21871..22080)
                     /gene="cspE"
                     /locus_tag="OHO85_001635"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21871..22080)
                     /gene="cspE"
                     /locus_tag="OHO85_001635"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCQ2671018.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22255..22815)
                     /gene="pagP"
                     /locus_tag="OHO85_001636"
     CDS             complement(22255..22815)
                     /gene="pagP"
                     /locus_tag="OHO85_001636"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCQ2671019.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23405..24790
                     /gene="dcuC"
                     /locus_tag="OHO85_001637"
     CDS             23405..24790
                     /gene="dcuC"
                     /locus_tag="OHO85_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCQ2671020.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24831..25511)
                     /gene="dpiA"
                     /locus_tag="OHO85_001638"
                     /gene_synonym="citB"
     CDS             complement(24831..25511)
                     /gene="dpiA"
                     /locus_tag="OHO85_001638"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCQ2671021.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(25480..27138)
                     /gene="dpiB"
                     /locus_tag="OHO85_001639"
     CDS             complement(25480..27138)
                     /gene="dpiB"
                     /locus_tag="OHO85_001639"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HCQ2671022.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVTNFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            27518..28576
                     /gene="citC"
                     /locus_tag="OHO85_001640"
     CDS             27518..28576
                     /gene="citC"
                     /locus_tag="OHO85_001640"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCQ2671023.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            28591..28887
                     /gene="citD"
                     /locus_tag="OHO85_001641"
     CDS             28591..28887
                     /gene="citD"
                     /locus_tag="OHO85_001641"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCQ2671024.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            28884..29792
                     /gene="citE"
                     /locus_tag="OHO85_001642"
     CDS             28884..29792
                     /gene="citE"
                     /locus_tag="OHO85_001642"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCQ2671025.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGAEVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            29803..31335
                     /gene="citF"
                     /locus_tag="OHO85_001643"
     CDS             29803..31335
                     /gene="citF"
                     /locus_tag="OHO85_001643"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCQ2671026.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            31339..31890
                     /gene="citX"
                     /locus_tag="OHO85_001644"
     CDS             31339..31890
                     /gene="citX"
                     /locus_tag="OHO85_001644"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HCQ2671027.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDDSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            31865..32743
                     /gene="citG"
                     /locus_tag="OHO85_001645"
     CDS             31865..32743
                     /gene="citG"
                     /locus_tag="OHO85_001645"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCQ2671028.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            32794..34257
                     /gene="citT"
                     /locus_tag="OHO85_001646"
     CDS             32794..34257
                     /gene="citT"
                     /locus_tag="OHO85_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCQ2671029.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            34371..35177
                     /gene="rna"
                     /locus_tag="OHO85_001647"
     CDS             34371..35177
                     /gene="rna"
                     /locus_tag="OHO85_001647"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCQ2671030.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            35407..35817
                     /gene="rnk"
                     /locus_tag="OHO85_001648"
     CDS             35407..35817
                     /gene="rnk"
                     /locus_tag="OHO85_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCQ2671031.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(36048..37286)
                     /locus_tag="OHO85_001649"
     CDS             complement(36048..37286)
                     /locus_tag="OHO85_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCQ2671032.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            37507..37935
                     /gene="uspG"
                     /locus_tag="OHO85_001650"
     CDS             37507..37935
                     /gene="uspG"
                     /locus_tag="OHO85_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCQ2671033.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(38056..39621)
                     /gene="ahpF"
                     /locus_tag="OHO85_001651"
     CDS             complement(38056..39621)
                     /gene="ahpF"
                     /locus_tag="OHO85_001651"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCQ2671034.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(39792..40355)
                     /gene="ahpC"
                     /locus_tag="OHO85_001652"
     CDS             complement(39792..40355)
                     /gene="ahpC"
                     /locus_tag="OHO85_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCQ2671035.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            40726..41472
                     /gene="dsbG"
                     /locus_tag="OHO85_001653"
     CDS             40726..41472
                     /gene="dsbG"
                     /locus_tag="OHO85_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCQ2671036.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT        10345 a        10597 c        10260 g        10331 t
ORIGIN      
        1 agttgcagca attacctgta ttagccaacc caagaaaacg acgttgcgtg ataaagccat
       61 ggtgaattat gcctttgatt atttaagatc accgggcagt cttccattca ccacggcagc
      121 cacggagctt tccgcgattc atggtcactc aacgtcgcaa tatcgccttg gagaatttta
      181 tcttcatggt agcgacggta aaccactgga ttatacacag gcgagatact ggtatgagca
      241 atcagcggaa caggaaaatc cacgcgcgca aagtaaactg gggtggatct acctcaaagg
      301 tctgggggtc aaacccgaca cccgtaaagc aattctctgg tataaggaag cagctgaaca
      361 agggtatgct catgctcaat atactttagg tttgatctac agaaatggct caggtattaa
      421 tgttaaccat tatgaatctc aaaaatggtt aaaactggcc gccaaacaac attacaaaaa
      481 tgcggaaaga ttacttgccg ggcttcccgc acattaacgt cgactgtcac ccgaacagat
      541 ggagtatttt ccctatcaaa ccagcaaaga taaaaatata gaaaataatt ttaatgatgc
      601 ccagacagcc tggcccacca tcgtcaacgg gcattggctg cggttgcggc tccggttcaa
      661 cagtttgctt ctcggtaaaa ttcgccgcct gaattaataa tcgacgaata attaatgctt
      721 ccagtggaca acgattttct gcatcaaggc ggctcacaat cggcattaat gattcttctg
      781 taggttggca ggcctgcact aattgtttgg caactagata taatggccat aagggatgtt
      841 ctgtatcacg cgtgagtgag atccgcggct tacgtccttt gtgcaaaacc atctcgacca
      901 gtgaacgccc cagcgcagag agtttttcat cgctgagtat ttcgtataaa tgaaaacgcg
      961 tacgggcgct gtcgccgggg acataaagta gcggttttcc ctgatcgtta accagatcac
     1021 gcgcttcaat catcatcacc agtaatgggt aataatcgac ccgatgctga cggcaccatt
     1081 gcaacattaa agcatccgcc tgggtggagg gatactcgcg ccagtggtca caaagttctg
     1141 ccaacaggct ttcgccttct ccacaataga cgcgttgcgc gtattcataa taaggcgcat
     1201 cttcattatc aggttcagct tcccgccatg actgggcgta atccagaagt tcggggattc
     1261 ccaagcggct tgcgctatac cagcggagta aatttctgtg cagttttgca tcatcgggga
     1321 tgatcactgc gcgatgttgc gctaaataca atgtcagcca ttctggatgc gatgaccaaa
     1381 tccgagcgaa catttggtaa aaatcgatgg tgtcattttg cacggcaacg ggcagatgca
     1441 gaatggtctg gaagttaaaa acattcccct tcgcaatggc atataaaaag gattccagtt
     1501 cttcttcgtc tatttccccg acgttttcct cctgcaacca ccgcaatctt tctgctaata
     1561 acaccacgca ctcgaaggat aaatgggcag tgttcatggc gattgtgcac agcgaccagc
     1621 gtaattcatc aatctcttcc atcgagcaat aatttaattg ctgaatgaat cgctgccagg
     1681 cggagggcag aaaacgttcg ctatcagagg caagtaacgc gcgaaaggcg agcagaattt
     1741 catgttctgc tacctcatat tcttctggtt gccaaacaga tttagcaggc gactgcgcaa
     1801 tccggagcgc ctcctcatac gcctgacgaa gttgtttaaa accctgcgga tcggtttctg
     1861 gatgaaagga cggtaacagc gccagataag cgcggcggat aatatcgacg tcagtcgttt
     1921 cctctatatc gaggatcttc cagcaatttt tcataggtcg ctcatatcct taagcgtttc
     1981 cgggcatgca gaatagtaag ccaatggttg ccaggacaag gtagggtaat acgcatcatc
     2041 actggcaaat aattgataaa acatatcatt gccggaatcg tcgtattctt tacgcgctaa
     2101 gaattcgcgt aataaaggca gatgatcatc ttcgggagac tgccaatatt gccgaccaaa
     2161 ggtataaccg gcaaagtatt gccgccagtt actataataa cgtagagctc gtagatgaat
     2221 gcgcgactgg atccatagac tttcttcttc gctcaaccag ttattaagta cgcccattcg
     2281 gcttaaaaaa cccatccgga cataatccca ggctttgatt cccccttcgc cacagcattc
     2341 ggcggtgctg gcgacaaatt gcgcataagc ctgaccttgc tcatttagtt cagcaagata
     2401 gtcacgccat tcacatggcg aataacgaaa ccagcggtga taaaaccctg ccaaccgagc
     2461 ggcatgaccg ttatcgatca ttcggtgaat tgtgcgcagc aggtcatcac ggtcatcaat
     2521 tccccatgag gaagaaaggt caaaaaagca tgtacctaaa tacattttgg gtgatgaatg
     2581 cgaaggtaaa cagcattcat tgcgtatttc catcggagtg gaaagtgcaa acaatatttt
     2641 ttgcgactcc atgtccatat aatttatcct taaatattca ttaaatgaat attagccgca
     2701 ttgcgaggct cgtttattta tacttttctg acgcgacata atacgcataa aataaggctt
     2761 acgcaaaaat ggaacacata aaaatataat atgttttatc tgcaaccata aggaaatgat
     2821 aatgattttc acgtcaactt gctgcgataa tttatcaata gatgaaatta tcgaacgtgc
     2881 tgaaaaaggc gattgcgagg ctcaatatat tgttgggttt tattataatc gcgatagcgc
     2941 aattgattct ccggacgacg aaaaagcctt ttactggctg aagctggccg ccgagcaagg
     3001 tcattgtgaa gcacaatatt ccttaggaca aaagtatact gaggataaaa gccgtcataa
     3061 agataatgag catgcaatct tttggctgaa aaaagctgcc ctacaaggcc atactttcgc
     3121 ttccaacgcc cttggctgga tactggatcg tggagaagac cccaattata aagaagcggt
     3181 tgtctggtat cagatagccg cggagagcgg aatgtcttat gcgcaaaata atcttgggtg
     3241 gatgtacaga aatggcaacg gagtcgcaaa agactatgcg ctggcatttt tctggtacaa
     3301 acaagctgca ttacaaggcc atagtgacgc gcaaaacaat ctggccgatc tttatgaaga
     3361 cggaaaaggc gttgctcaaa acgagacact cgccgcattc tggtatttga aaagcgcaca
     3421 gcagggtaat cggcatgctc agtttcaaat tgcatgggat tataacgctg gcgaaggggt
     3481 ggaccaggac tataagcaag cgatgtactg gtatctgaag gctgccgctc aggggagcgt
     3541 cggcgcttac gtcaacatcg gttatatgta taaacacgga caaggcgttg agaaagatta
     3601 tcaggctgcc tttgaatggt ttacgaaagc cgctgaatgc aatgacgcca ctgcctggta
     3661 taacctggcc attatgtatc actacggaga aggaagacct gtcgatctcc gacaggctct
     3721 cgacctgtat cgtaaagttc agtcatccgg aaccagagat gtcagtcagg aaattcgtga
     3781 gactgaagat ttactgtaga gataaaaaca agggccgggg aatttcattc ggcccatatt
     3841 ctctttattg ctgtgcgaaa tcagactatt acggctcaaa cgggcgtctc tggaactggt
     3901 catgaccaca tttcgggcat agcgtcagca cttccggtgt gtagatcggg agatggaagt
     3961 gacatttctc gcagaccaga tttcccagcc cgaccacttc tccgctgtga taaaccccat
     4021 gatgattgag gtcctggaaa acttcgcgcc attcaagctg cgttttatcg gtgatatctg
     4081 ccagctcctg ccataagctt tctttaatca cccgcataaa gacgctgtca gattcttctt
     4141 tcaggctctc ttcatagctc atggcgaact cttccaggtc acgtcttaca gctcgcgtca
     4201 gctcatcgac ctcggttcgc gttaactccc ctgtttttat tacgcgctcg cgcgcctgtt
     4261 ccaccagtgc gtcgatatca cgttcgccat tgcgcaggcg ttcgctcagt gacgcgacca
     4321 gttcacggta atattgagca accttgttca tcgtttcatc tcccgggtcg ttaactgact
     4381 ataataatag acgttctttg ttccttgctt tgctaatacg cccacaaact ctgtaaatta
     4441 atgtatgaat aatccgggca tcatttcaac gtcgagtgcg cgaaaggctg ttttgacgcg
     4501 gacgtttggg ctatgctatg cggatctgaa aaaccacctc aacgctacat ttgtagccgt
     4561 attgaaaaca ggaccactgg ctgccatgca agagcaatac cgcccggaag agatagaatc
     4621 caaagtacag cttcattggg atgagaagcg cacatttgaa gtaaccgaag acgagagcaa
     4681 agagaagtat tactgcctgt ctatgcttcc ctatccttct ggtcgactac acatgggcca
     4741 cgtacgtaac tacaccatcg gtgacgtgat cgcccgctac cagcgtatgc tgggcaaaaa
     4801 cgtcctgcag ccgatcggct gggacgcgtt tggtctgcct gcggaaggcg cggcggtgaa
     4861 aaacaacacc gctccggcac cgtggacgta cgacaacatc gcgtatatga aaaaccagct
     4921 caaaatgctg ggctttggtt atgactggag ccgcgagctg gcaacctgta cgccggaata
     4981 ctaccgttgg gaacagaaat tcttcaccga gctgtataaa aaaggcctgg tatataagaa
     5041 gacttctgcg gtcaactggt gcccgaacga ccagaccgta ctggcgaacg aacaagttat
     5101 cgacggctgc tgctggcgct gcgataccaa agttgaacgt aaagagatcc cgcagtggtt
     5161 tatcaaaatc actgcttacg ctgacgagct gctcaacgat ctggataaac tggatcactg
     5221 gccagacacc gttaaaacca tgcagcgtaa ctggatcggt cgttccgaag gcgtggagat
     5281 caccttcaac gttaacgact atgacaacac gctgaccgtt tacactaccc gcccggacac
     5341 ctttatgggt tgtacctacc tggcggtagc tgcgggtcat ccgctggcgc agaaagcggc
     5401 ggaaaataat cctgaactgg cggcctttat tgacgaatgc cgtaacacca aagttgccga
     5461 agctgaaatg gcgacgatgg agaaaaaagg cgtcgatact ggctttaaag ctgttcaccc
     5521 attaacgggc gaagaaattc ccgtttgggc agcaaacttc gtattgatgg agtacggcac
     5581 gggcgcagtt atggcggttc cgggtcacga ccagcgcgac tacgagtttg cctctaaata
     5641 cggcctgaac atcaaaccag ttatcctggc tgctgacggc tctgagccag atctttctca
     5701 gcaagccctg actgaaaaag gcgtgctgtt caactctggc gaattcaacg gtcttgacca
     5761 tgaagcggcc ttcaacgcca tcgccgataa actgactgcg atgggcgttg gcgagcgtaa
     5821 agtgaactac cgcctgcgcg actggggtgt ttctcgtcag cgttactggg gcgcgccgat
     5881 tccgatggtg acgctggaag acggtaccgt aatgccgacc ccggacgacc agctgccggt
     5941 gatcctgccg gaagatgtgg tgatggacgg cattaccagc ccgattaaag cagatccgga
     6001 gtgggcaaaa actaccgtta acggtatgcc agcgctgcgt gaaaccgaca ctttcgacac
     6061 ctttatggag tcctcctggt actatgcgcg ctacacttgc ccagagtaca aagaaggtat
     6121 gctggattcc gaagcggcta actactggct gccggtggat atctacattg gtggtatcga
     6181 acacgccatt atgcacctgc tctacttccg cttcttccac aaactgatgc gtgatgcagg
     6241 catggtgaac tctgacgaac cagcgaaaca gttgctgtgt cagggtatgg tgctggcaga
     6301 tgccttctac tatgttggcg aaaacggcga acgtaactgg gtttccccgg ttgatgctat
     6361 cgttgaacgt gacgagaaag gccgtatcgt gaaagcgaaa gatgcggcag gccatgaact
     6421 ggtttatacc ggcatgagca aaatgtccaa gtcgaagaac aacggtatcg acccgcaggt
     6481 gatggttgaa cgttacggcg cggacaccgt tcgtctgttt atgatgtttg cttctccggc
     6541 tgatatgact ctcgaatggc aggaatccgg tgtggaaggg gctaaccgct tcctgaaacg
     6601 tgtctggaaa ctggtttacg agcacacagc aaaaggtgat gttgcggcac tgaacgttga
     6661 tgcgctgact gaagatcaga aagcgctgcg tcgcgatgtg cataaaacga tcgctaaagt
     6721 gaccgatgat atcggccgtc gtcagacctt caacaccgca attgcggcga ttatggagct
     6781 gatgaacaaa ctggcgaaag caccaaccga tggcgagcag gatcgcgctc tgatgcagga
     6841 agcgctgctg gccgttgtcc gtatgcttaa cccgttcacc ccgcacatct gcttcacgct
     6901 gtggcaggaa ctgaaaggcg aaggcgatat cgacaacgcg ccgtggccgg ttgctgacga
     6961 aaaagcgatg gtggaagact ccacgctggt cgtggtgcag gttaacggta aagtccgtgc
     7021 caaaatcacc gttccggtgg acgcaacgga agaacaggtt cgcgaacgtg ctggccagga
     7081 acatctggta gcaaaatatc ttgatggcgt taccgtgcgt aaagtgattt acgtaccagg
     7141 taaactcctc aatctggtcg ttggctaagc gcgggaggaa gcgtgcgata tctggcaaca
     7201 ttgttgttat ctctggcggt gttaatcacc gccgggtgtg gctggcatct gcgtgatacc
     7261 acgcaggttc cttccactat gaaggtcatg atcctggact caggcgatcc gaacgggcca
     7321 ttaagccgtg cggtgcgtaa ccagttacgt ctgaatggtg tcgagttgct tgataaagaa
     7381 accacgcgta aggacgttcc atccctgcgt ttgggtaaag tgagcatcgc gaaagatacc
     7441 gcatcggtat tccgtaacgg tcaaacagca gagtatcaga tgatcatgac ggtgaatgcg
     7501 accgtgttga tccccggccg tgatatctac ccgattagcg ccaaagtctt ccgttcgttc
     7561 tttgataacc cgcaaatggc gttagcgaaa gataacgaac aagacatgat cgtaaaagag
     7621 atgtacgacc gtgctgccga acagctgatt cgtaagctgc caagcatccg tgctgcggat
     7681 attcgttccg acgaagaaca gacgtcgacc acaacggata ctccggcaac gcctgcacgc
     7741 gtctccacca cgctgggtaa ctgatgattc ggttgtaccc ggaacaactc cgcgcgcagc
     7801 tcaatgaagg gctgcgcgcg gcgtatcttt tacttggtaa cgatcctctg ttattgcagg
     7861 aaagccagga cgctgttcgt caggtagctg cggcacaagg attcgaagaa caccacactt
     7921 tttccattga tcccaacact gactggaatg cgatcttttc gttatgccag gctatgagtc
     7981 tgtttgccag tcgacaaacg ctattgctgt tgttaccaga aaacggaccg aatgcggcga
     8041 tcaatgaaca acttctcaca ctcaccggac ttctgcatga cgacctgctg ttgatcgtcc
     8101 gcggtaataa attaagcaaa gcgcaagaaa atgccgcctg gtttactgcg cttgcgaatc
     8161 gcagcgtgca ggtgacctgt cagacaccgg agcaggctca gcttccccgc tgggttgctg
     8221 cgcgcgcaaa acagctcaac ttagaactgg atgacgctgc aaatcaggtg ctctgctact
     8281 gttatgaagg taacctgctg gcgctggctc aggcactgga gcgtttatcg ctgctctggc
     8341 cagacggcaa attgacatta ccgcgcgttg aacaggcggt gaatgatgcc gcgcatttca
     8401 ccccttttca ttgggttgat gctttgttga tgggaaaaag taagcgcgcg ttgcatattc
     8461 ttcagcaact gcgtctggaa ggcagcgagc cggttatttt gttgcgcaca ttacaacgtg
     8521 aactgttgtt actggttaac ctgaaacgcc agtctgccca tacgccactg cgtgcgttgt
     8581 ttgataagca tcgggtatgg cagaaccgcc ggggcatgat gggcgaggcg ttaaatcgct
     8641 taagtcagcc gcagttacgt caggccgtgc aactcctgac acgaacggaa ctcaccctca
     8701 aacaagatta cggtcagtca gtgtgggcag agctggaagg gttatctctt ctgttgtgcc
     8761 ataaacccct ggcggacgta tttatcgacg gttgatatga aatctttaca ggctctgttt
     8821 ggcggcacct ttgatccggt gcactatggt catcttaaac ccgtggaaac gctggcgaat
     8881 ttgattggtc tgacgcgggt cacaatcatc cctaataatg ttcctccgca tcgtccccag
     8941 ccggaagcga acagcgtgca gcgtaaacac atgcttgaac tggcgattgc cgacaagcca
     9001 ttatttactc ttgatgaacg cgagctaaag cgcaatgccc cctcttacac tgcgcaaaca
     9061 ctgaaagagt ggcggcagga acaagggccg gacgtgccgc tggcgtttat tattggtcag
     9121 gattcattgc tgacctttcc gacctggtac gaatacgaaa cgatactcga caatgcacat
     9181 ttgatcgtct gtcggcgtcc aggttaccca cttgaaatgg cgcaaccgca ataccagcaa
     9241 tggctggaag atcatttgac acataacccg gaagatcttc accttcagcc tgccggtaaa
     9301 atttatctgg ctgaaacgcc gtggtttaac atctcggcga ccatcatccg cgaacgtttg
     9361 caaaacggtg aatcgtgtga ggatttattg ccggaaccgg tactgactta cattaaccaa
     9421 caaggcttgt atcgctgata cctgtcgtta cggagggcat gatgcgactg tggttaattc
     9481 gtcatggcga aacgcaagcg aatgtcgatg gtctgtacag cggtcatgcg cccacacccc
     9541 tgaccgcacg cggtattgag caagcgcaaa atctgcatac gctgctacat ggtgtttcct
     9601 ttgatctggt tttatgcagt gaactggaac gggcacagca taccgcgcga ctggttctca
     9661 gtgaccgcca gctccccgtg caaatcatcc ctgaactcaa cgaaatgttt tttggcgact
     9721 gggagatgcg acatcatcgc gacctcatgc aagaagatgc cgaaaactat agcgcgtggt
     9781 gcaatgactg gcagcatgca atccccacga acggtgaagg atttcaggca ttttcgcaac
     9841 gtgtggaacg ctttatcgca aggcttagtg aatttcagca ctatcagaat attttagtcg
     9901 tcagccatca gggtgtactg agtctgttaa tcgcccgttt aattggcatg cctgccgagg
     9961 ctatgtggca ttttcgcgtt gaccaggggt gttggagcac cattgatatc aaccaaaaat
    10021 tcgcgacgct acgcgtcctc aatagccgtg ccatcggggt cgagaatgca tgacttttct
    10081 gtttttttac gggtaagccg caacgaccat tgacagaccc gggcaggctg atattctccg
    10141 cagccagact tttttcgcca gacacgactt tgtagaaatt gttttacaaa aatggcgatg
    10201 caatctgcgg cgcggggtgg gatgatagcc cactttcgaa agccgattcg gcgacaattg
    10261 tcccgaaatc gcctctggtt caggtatact gacagaccat ttttatctat ttgattcacc
    10321 cagggggaaa acttgcaggg taaagcactc caggattttg ttatcgacaa aattgatgac
    10381 ctcaaaggtc aggacatcat cgccttagac gttcaaggca aatccagcat caccgactgc
    10441 atgatcatct gtacgggtac gtccagccgt catgttatgt ccattgctga ccacgttgtg
    10501 caggagtctc gcgcagcggg cctgttaccg ctcggcgtag aaggtgaaaa cagcgccgac
    10561 tggattgtcg tggatttggg cgatgtgatt gtccatgtca tgcaggaaga gagccgtcgc
    10621 ctgtatgaac tggaaaaact ctggagttaa tgcgtgaagc tgcaacttgt cgccgtggga
    10681 acgaaaatgc cggactgggt acaaaccggt tttaccgagt acctgcgtcg ttttccgaaa
    10741 gatatgccct tcgagctgat tgaaattccg gccggaaaac gcggcaagaa tgcggacatc
    10801 aagcgcatac tcgacaaaga gggtgagcag atgttggcgg ccgcaggcaa aaaccgcatt
    10861 gtcaccctcg atattccagg caagccctgg gatacaccgc agttagccgc tgagctggaa
    10921 cgctggaagc tggatggtcg cgacgtcagt ctactgattg gcgggcctga agggttgtcg
    10981 cctgcctgta aagcggcggc ggagcagagc tggtcgctgt cggcgcttac cctcccccat
    11041 ccgctggttc gcgtgctggt cgcagagagt ctgtaccggg cgtggagcat caccaccaac
    11101 catccttatc accgtgagtg ataagggagc tttgagtaga aaacgcagcg gatgaaacta
    11161 cagaactctt ttcgcgacta tacggcagag tccgcgctgt ttgtgcgccg ggcgctggtc
    11221 gcatttttgg ggattttgct gctgaccggc gtgcttatcg ccaacctgta taatctgcaa
    11281 attgttcgct ttaccgacta ccagactcgc tctaatgaaa accgcattaa gctggtgcct
    11341 atcgcgccca gccgcggcat tatctacgac cgtaatggta tccctctggc cctcaaccgc
    11401 actatctacc agatagaaat gatgccagag aaagtcgata acgtgcagca aacgctggac
    11461 gctttgcgca gcgtggttga tctgaccgat gacgatattg ctgcattccg aaaagagcgc
    11521 gcacgttcac accgtttcac ctctattccg gtgaaaacta acctgaccga agtacaagta
    11581 gctcgctttg ccgtcaatca gtaccgtttt ccgggtgtcg aagttaaagg ctataaacgt
    11641 cgttactatc cttacggttc ggcgttgacc cacgtcatcg gctatgtgtc gaaaatcaac
    11701 gataaagacg tcgaacgcct gaataatgac ggcaaactgg ccaactatgc ggcaacgcat
    11761 gatatcggta agctgggcat tgagcgttac tatgaagacg tgctgcacgg tcagaccggt
    11821 tatgaagagg ttgaagttaa caaccgtggg cgtgttatcc gccagttaaa agaagtacca
    11881 ccgcaagccg gacacgatat ttacctgacg ctggatctca aactccagca atatattgaa
    11941 acgctgctgg cgggtagccg cgcagctgtg gttgtcaccg atccgcgtac aggtggggtg
    12001 ctggcgctgg tttccacgcc tagttatgac ccaaacctgt ttgttgacgg tatctccagc
    12061 aaagattatt ccgccttgtt gaacgacccg aatacaccgc tggtgaaccg cgccacacag
    12121 ggggtttatc ctcccgcgtc tacagttaaa ccctatgtgg cggtttcggc attgagcgcc
    12181 ggggtgatca cgcgcaatac gacgctgttt gacccaggct ggtggcaact gccaggttcg
    12241 gaaaaacgtt atcgtgactg gaaaaaatgg ggccacgggc gtctgaatgt cacaagatcg
    12301 ctggaagaat ctgcggatac cttcttctat caggtggcct acgatatggg gatcgatcgc
    12361 ctctccgaat ggatgggtaa attcggttat ggtcattaca ccggtatcga cctggcggaa
    12421 gaacgttccg gcaacatgcc tacccgcgaa tggaaacaga aacgctttaa aaaaccgtgg
    12481 tatcagggtg acaccattcc ggttggtatc ggtcagggtt actggacagc gaccccaatc
    12541 cagatgagta aggcactgat gatcctgatt aatgacggta tcgtgaaggt tccgcatttg
    12601 ctgatgagca ccgccgaaga cggcaaacag gtgccatggg tacaaccgca tgaaccgccc
    12661 gtcggcgata ttcattccgg ttactgggag ctggcgaaag acggtatgta cggtgttgct
    12721 aaccgcccta acggtacggc gcataaatac tttgccaacg caccgtacaa aattgcggcg
    12781 aaatctggta ccgctcaggt cttcggtctg aaagcgaacg aaacctataa tgcgcacaaa
    12841 attgccgagc gtttacgtga ccacaaactg atgaccgcct ttgcgccata caacaatccg
    12901 caagtggctg tcgccatgat tctggagaac ggcggcgcgg gtccggcggt tggtacgctg
    12961 atgcgccaga tcctcgacca cattatgctg ggtgataaca acaccgatct gcctgcggaa
    13021 aatccagcgg ttgccgcagc ggaggaccat taatcatgac ggataatccg aataaaaaaa
    13081 cattctggga taaagtccat ctcgatccca caatgctgct gattttactg gcattgctgg
    13141 tttacagcgc cctggttatc tggagcgcca gcggtcagga tattggcatg atggagcgca
    13201 aaatcggcca aatcgcgatg ggtctggtca tcatggtggt gatggcgcaa attcctccac
    13261 gcgtttatga aggctgggcc ccctatctct atatcatctg tattattttg ctggtggcgg
    13321 tagatgcttt cggtgccatc tctaaaggtg ctcaacgctg gctggacctc ggtattgttc
    13381 gttttcagcc gtcggaaatt gccaaaatag ccgtaccact gatggttgcg cgctttatca
    13441 accgcgacgt ttgcccgcca tcgttgaaga acactggcat cgcgctggtg ctgatattta
    13501 tgcccacgct gctggtggct gcacagcctg acctgggaac atcaatcctc gttgcgcttt
    13561 ccggtctgtt tgtactgttc ctctctggcc ttagctggcg tctgattggc gtcgcagtag
    13621 tgctggtagc ggcgttcatt ccgattctgt ggttcttcct gatgcatgat taccagcgcc
    13681 agcgcgtaat gatgctcctg gacccggaat cagacccact aggcgcgggc tatcacatta
    13741 ttcagtctaa aattgctatt ggctccggcg gattacgcgg caaaggctgg ctgcacggca
    13801 ctcagtcaca gcttgaattt ctccccgaac gccataccga ctttatcttc gcggtactgg
    13861 cggaagagct gggattagtg ggcattctga ttctgctcgc tctctacatt ctgctgatca
    13921 tgcgcgggct gtggatagcc gccagagcgc aaaccacctt tggtcgcgtc atggctggtg
    13981 gcttaatgct gatattattc gtttatgtct tcgtaaatat tggtatggta agcggtattc
    14041 tgccggttgt aggggttccg ctcccactgg tcagttatgg aggatcggcg ctaattgtgc
    14101 tgatggctgg gttcgggatt gtaatgtcaa tccacaccca caggaaaatg ttgtcgaaaa
    14161 gcgtgtaaga ggtgcgcaat gcgtaagcag tggctcggga tctgcatcgc ggcaggaatg
    14221 ctcgcggcat gtacaagcga tgatggtcag caacagacgg taagtgtacc gcagcctgcg
    14281 gtatgtaacg gccccatagt tgaaattagc ggggcggacc cgcgtttcga accactgaac
    14341 gcgacggcaa atcaggatta ccagcgcgac ggtaaaagct acaaaatcgt gcaggatccg
    14401 tctcgattta gccaggcggg actggcggca atctatgatg ccgaacctgg cagtaacctg
    14461 acggcctctg gcgaagcttt cgatccgaca cagttgacgg cggcccatcc aacgcttccg
    14521 atccccagct acgccagaat cactaacctg gctaacgggc gaatgatcgt ggtgcgcatt
    14581 aatgatcgcg gcccctacgg caacgaccgc gtaatttcgc tttctcgcgc agcagctgac
    14641 cgtcttaaca cgtcaaacaa caccaaagtt cgtatcgatc cgattattgt cgcccaggat
    14701 ggttcgcttt ctggtcctgg tatggcttgt accacagtcg ccaaacagac ttacgccctg
    14761 cctgcacctc ccgatttaag cggtggcgcg ggggcaagtt cagtgtctgg cccgcagggt
    14821 gacattcttc cggtcagtaa ctcgacgcta aaaagcgaag atccgaccgg cgcgccggta
    14881 accagcagcg gtttcctcgg cgcaccaacg accttagcgc ctggtgtact ggaaagcagc
    14941 gaaccgacac ctgcaccaca gcccgttgtt acagctccgt cgacaacgcc tgcaacctcg
    15001 cctgcaatgg tgacaccgca agccgcctcg caaagcgcca gcggcaactt tatggtgcaa
    15061 gtcggggccg ttagcgatca ggctcgtgcg caacagtacc aacagcaact gggacagaag
    15121 ttcggcgtcc ccggtcgcgt aactcaaaat ggcgcggtct ggcgtatcca gcttggccca
    15181 ttcgccagta aagccgaagc cagtaccttg cagcaacgtt tgcaaaccga agcccaatta
    15241 cagtcattta ttaccaccgc gcagtagcgt aaagcaggca tctgatgtgt caatttctgt
    15301 aaatgacgtt aacaaagtca cgcggaaagt cagatgcctg ccggtagtgc atttgctata
    15361 gtagggcact ttttttaatt ccatcacgga tgtcgtagtt cagaccatga ataccatttt
    15421 ttccgctcgt atcatgaagc gcctggcgct caccacggct ctttgcacag cctttatctc
    15481 tgctgcacat gccgatgacc tgaatatcaa aactatgatc ccgggtgtac cgcagatcga
    15541 tgcggagtcc tacatcctga ttgactataa ctccggcaaa gtgctcgccg aacagaacgc
    15601 agatgtccgc cgcgatcctg ccagcctgac caaaatgatg accagttacg ttatcggcca
    15661 ggcaatgaaa gccggtaaat ttaaagaaac tgatttagtc actatcggca acgacgcatg
    15721 ggccaccggt aacccggtgt ttaaaggttc ttcgctgatg ttcctcaaac cgggcatgca
    15781 ggttccggtt tctcagctga tccgcggtat taacctgcaa tcgggtaacg atgcttgtgt
    15841 cgccatggcc gattttgccg ctggtagcca ggacgctttt gttggcttga tgaatagcta
    15901 tgttaacgcc ctgggcctga aaaacaccca cttccagacg gtacatggtc tggatgctga
    15961 tggtcagtac agctccgcgc gcgatatggc gctgatcggc caggcgttga tccgtgacgt
    16021 accgaatgaa tactcgatct ataaagaaaa agaatttacg tttaacggta ttcgccagct
    16081 gaaccgtaac ggcctgttat gggataacag cctgaatgtc gacggcatca aaaccggaca
    16141 cactgacaaa gcaggttaca accttgttgc ttctgcgact gaaggccaga tgcgcttgat
    16201 ctctgcggtg atgggcggac gtacttttaa aggccgtgaa gccgaaagta aaaaactgtt
    16261 aacctggggc ttccgtttct tcgaaaccgt taacccactg aaagtaggta aagagttcgc
    16321 ctctgaaccg gtttggtttg gtgattctga tcgcgcttcg ttaggggttg ataaagacgt
    16381 gtacctgacc attccgcgtg gtcgcatgaa agatctgaaa gccagctatg tgctgaacag
    16441 cagtgaattg catgcgccgc tgcaaaagaa tcaggtcgtc ggaactatca acttccagct
    16501 tgatggcaaa acgatcgagc aacgcccgct ggttgtgttg caagaaatcc cggaaggtaa
    16561 cttcttcggc aaaatcattg attacattaa attaatgttc catcactggt ttggttaaaa
    16621 attgaacact tgaaagtgta atttccgtcc ccatatacta agcatcagta aaaaaactcc
    16681 cgccttctgg cgggagttgc tatttaatta cgttacgccg gagctgacat gaaaaccaaa
    16741 cttaacgaac tgcttgaatt ccctactcct tttacttaca aagttatggg gcaggcgtta
    16801 cctgagctgg ttgatcaggt ggttgaagtg gtacagcgcc atgcgccagg tgactacacc
    16861 ccaacggtaa aaccaagcag caaaggcaac taccactcgg tatctatcac tatcaacgcc
    16921 actcatatcg agcaggttga aacactgtat gaagaactgg gcaaaatcga tattgtccgc
    16981 atggttctgt aactcgcttc tccgttaccc ggctcctggt cgggtaactc ccctctcctc
    17041 gctgtgatat acttttcccc cacttttact cattctccac ggagatgccg ttttgtatca
    17101 ggataaaatt cttgtccgcc agctcggtct tcagccttac gagccaatct cccaggctat
    17161 gcatgaattc accgataccc gcgatgatag tacccttgat gaaatctggc tggtcgagca
    17221 ctatccggta ttcacccaag gtcaggcagg aaaagcggag cacattttaa tgccgggtga
    17281 tattccggtg atccagagcg atcgcggtgg gcaggtgact tatcacgggc cgggacaaca
    17341 ggtgatgtat gtgttgctta acctgaaacg ccgtaaactc ggtgtgcgtg aactggtgac
    17401 cttgcttgag caaacagtgg tgaataccct ggctgaactg ggtatagaag cgcatcctcg
    17461 ggctgacgcg cctggtgtct atgtcgggga aaagaaaatt tgctcactgg gtttacgaat
    17521 tcgacgcggt tgttcattcc acggtctggc attaaacgtc aatatggatc tttcaccatt
    17581 tttacgtatt aatccttgtg ggtatgccgg aatggaaatg gctaaaatat cacaatggaa
    17641 acccgaagcg acgactaata atattgctcc acgtttactg gaaaatattt tagcgctact
    17701 aaacaatccg gacttcgaat atattaccgc ttaattccat acatcaatgg cccaatttac
    17761 actgggtcat tactcataat acctgctaat gctttacatc tccttcgcgt ttgtatattt
    17821 tctttatcac ttgcagaaat gcggtgccac gccttgctcc gtaactcacc ccattgatta
    17881 acctattgaa atagttgatt atggtgagca aaagcgttcc gttccaatta gacataagat
    17941 tattgtaatt ttttaaatat tatttttcca tgaataaaat tggagtaagt gcgtggatag
    18001 taataatcaa attgaaccct gtttaagcag aaagtcgtca gaaggtaaac cacaaatatt
    18061 tacaacctta cgaaatattg atcttaacct tctgactatt tttgaagctg tatatgtaca
    18121 taaagggatc gttaatgcag cgaaagtgct taatctgacc ccctcggcaa tcagtcagtc
    18181 tattcagaaa ctgcgcgtta tattccctga cccattgttt attcgcaaag gccagggtgt
    18241 cactcctacc gcatttgcga tgcatctaca tgagtatatc agtcagggcc ttgagtccat
    18301 tcttggcgcg ctggatatcg aaggaagcta tgataagcaa cgaacgataa ctattgcgac
    18361 cactccctcg gtcggagccc tggtccttcc tgtcatctat cgggcgatta aaactcacta
    18421 tccgcagctt ttactgcgca acccgcccat cagcgacgcg gaaaaccaac tcagtcagtt
    18481 tcaaaccgat ctcatcatcg ataacatgtt ttgcaccaat cgtacggtgc aacatcatgt
    18541 tctgttcacc gacaatatgg tgttaatttg ccgcgaggga aatccactac tctctttaga
    18601 agatgacaga gagactatcg acaacgctgc gcatgtactt ctgttaccgg aagagcaaaa
    18661 tttcagcggt ctgcggcaga gagttcaaga aatgtttccg gaccggcaaa tcaatttcac
    18721 cagctacaac attttgacaa tcgctgcact ggttgccaac agtgacatgt tagcgattat
    18781 tcccagccgt ttttataacc tgtttagccg ctgctggccg ctggaaaaac tgccttttcc
    18841 gtccttaaat gaggagcaaa tagatttctc tatccactac aacaaattca gcctgcgtga
    18901 tcccatcctg cacggagtaa tagatgttat ccgtaatgca ttttgaaaaa gtattgttac
    18961 agccagatca cagcgcagaa cgataaaatg gcacaatcca acaacaattt tacattttcg
    19021 cgaccgcttt ggctgctttc aggtccgttt caatgatata ctgccagtcg ttaattcaaa
    19081 aatagttgat aattacaaca atctattgaa ttgaaacgct ttccttcgta attcgcaact
    19141 ggaacacgca cgctatgagt aaacccattg tgatggaacg cggtgttaaa taccgcgatg
    19201 ccgataagat ggcccttatc ccggttaaaa acgtggcaac agagcgcgaa gccctgctgc
    19261 gcaagccgga atggatgaaa atcaagcttc cagcggactc tacacgtatc cagggcatca
    19321 aagccgcaat gcgcaaaaat ggcctgcatt ctgtctgcga ggaagcctcc tgccctaacc
    19381 tggcggaatg cttcaaccac ggcacagcaa cgtttatgat cctcggcgct atttgtaccc
    19441 gccgttgtcc gttctgtgat gttgcccacg gtcgcccggt agctcctgat gccaatgaac
    19501 cagtgaaact ggcgcagacc attgccgata tggcgctgcg ttatgtggtt atcacctccg
    19561 ttgaccgtga tgacctgcgc gatggcggtg cccagcactt tgcggattgc attactgcca
    19621 ttcgggaaaa aagcccgcaa atcaaaattg aaactctggt gccggatttc cgtggtcgta
    19681 tggatcgtgc tctggatatt ctgactgcaa cgccaccaga tgtgttcaac cataacctgg
    19741 aaaacgtacc gcgtatttac cgtcaggtac ggcctggtgc agattacaac tggtcgctga
    19801 agctgctgga acgctttaaa gaagcgcatc cggaaatccc gaccaagtct ggtctgatgg
    19861 tgggactggg tgaaaccaat gaagaaatta ttgaggtaat gcgcgacctg cgccgtcatg
    19921 gtgtgacgat gttaacgctg gggcaatatt tgcagccaag ccgccatcac ctgccggttc
    19981 aacgttacgt tagcccggat gagttcgacg aaatgaaagc cgaagcgctg gcgatgggct
    20041 ttacccatgc tgcatgcggt ccgtttgtcc gctcttctta ccacgccgat ttgcaggcga
    20101 aagggatgga agttaagtaa tcctgtaata attacttaca tctgtctgat aaaaaacccg
    20161 ctttttgaag cgggtttttt gtatcgaaca agatattgag ggagcgtcct gctcgccacg
    20221 tcactcttta tgagagagct tttcagcctg aagatcaacg tctgcgcctt ttttcgccgc
    20281 agcatcgtca tcattcatcg ccttcttgaa ccctttaatg gccgctccaa ggtctccgcc
    20341 cagcgtacgt aacttcttag tcccaaacag cagaacgacc agcgccgcaa ctaccagcag
    20401 tttggtaata ctaatctcac ccatagatac cttcttgaca tataaccggc aacagaacgc
    20461 gccttaaaaa ccagagaata ttaacggtca ttcgacgcgc gcacacaata gcaatctgta
    20521 acaaggtgaa tcaagcgccg agtagaaaaa catcataata attgcggcgg cgcaaagcga
    20581 cggttgttta agacgggcag ttgcgcgcgc acctgacgca cacgttcggg cgtcacttcc
    20641 gccataatga gtgcaggcat ttctgacgct gccgcaatgg tgacgccaaa gggatctata
    20701 atccggcttt gaccgatatt tttgttcccg cactcccccg ccgccaccat ataacaggtg
    20761 gtatccagcg cacgagcggc aagcaacgtt gaccagtgat gctctttgag cggcccgcga
    20821 acccaggcgg caggaagtac caggatttca gctccctgta atgcctgtgc cagcgccagc
    20881 tctggaaagc gtaagtcata acaggtcatc agaccgacct tcatcccttc cacctccagt
    20941 aacggagcga tttcattacc agcatcaaca cggcgtgatt cctgaatggc aaatgcatca
    21001 tagagatgca gtttggcata acgggcgacg atgtttcctg cctgaagtgc caccagcata
    21061 ttccatgcgc gccccggcgt tgaaggaaca tgaatcgtca gaattgtcgt catcatgtta
    21121 cgtttacttt ctcgccgtaa acgtccgagg aattcgcctt ccagcagctg tgctgattta
    21181 accgatagat ctgcatcatg atcatcgcgc gccagcaatg cttccggcag gacaaacagc
    21241 gatacgtcgt tttccgccgc ctgcgccatc aacgaggcac aaatctcagc gttcttttcc
    21301 cacacagatg taacagcaaa ctgtcctgcg gcaaccagca ttgccacctc ctgtcagagt
    21361 cgtaaaattc gccaacgacg attattcggc gttacactta tcactcatac aaatcaaata
    21421 gcaggatttt gcagtgttac aacttctttt agcagttttt attggcggtg gtacgggaag
    21481 cgtggcgaga tggctgttaa gtatgcgatt taacccactg catcaggcga ttccgttggg
    21541 gacgctgaca gcaaacctga ttggggcatt catcatagga atgggattcg catggttcag
    21601 caggatgacg aacattgatc cagtgtggaa agtattaatc accaccggat tttgtggcgg
    21661 tctaacaacc ttctcaacat tttcggcaga agtggtgttt ttgttacaag agggccgctt
    21721 tggctgggca ttactgaacg ttttcgtcaa ccttctgggg tcttttgcca tgaccgcact
    21781 ggcattctgg ctgttttcgg cctcaaccgc acactaaaga ccaaaaaaaa cccgctgatt
    21841 aagcgggttt tgaattcttg ctgacgtatc ttacagagcg attacgtttg cagcagaagg
    21901 gcctttggca ccgttagtga tttcgaactc tacgcgctga ccttcagcaa gagttttaaa
    21961 accattagtc tggattgcag agaagtgtac gaacacgtct ttgctgccgt cttccggagt
    22021 aatgaaaccg aatcctttgg actcattaaa ccacttaacg ttacctttaa tcttagacat
    22081 caaaattacc tttacatgaa aaatagacac aaatgctgtg tcggttacca gtacaccaat
    22141 tgtggtacgc tttgtccagt gcaactttac taaaaagtga taaaagtcgc gttatttttg
    22201 atgttactta ttttagctat tgattttaaa gaagttacta aaacttcatt tgcctcaaaa
    22261 ctgaaagcgc atccaggcaa agtacacatt gccattgttg taggtacccg gaatgtaggt
    22321 catctgaaaa gtcactgggc cataacccac ggaggccaat ggcagtagaa ccgggagagg
    22381 gatgtaattc cagttatcgc gtgccgttac gccagcggtg aatcccagac ctaaatgaaa
    22441 attttcatcc gccagcggtc gccaggtact ttcccatccg tatccggcaa tcggttccca
    22501 tttgttccac gagtccttaa atgccatggc atacaggcca tgccagtttc ctttttcatc
    22561 ccaacgcgac aggccaaaac cgccacccca cggtcgctcg ttatagcgat cggttttttc
    22621 tttgtcgtaa gcgaaacgtg catgccaggt gatggcagga atatataaat cataatgttc
    22681 aggctgttgc caggtttgtg caatattttc tctaaacgtt gtcatccact catctgcgtt
    22741 agcaaaaact ttaccaacgc tgattaactg aataaaaaca aaggaaaaga tagcgacata
    22801 tttactcacg ttcatttgtg accataaaac atttatcaaa aatctactac tagcatagca
    22861 aagctacaat taacataacc ttaatagacc caacataaag aataatctga atagctgctc
    22921 tatttatttc cgcttgttca agttaaatat ttccgcgcat cgattaaaga tgatgcgtcg
    22981 ttttctgcga tgggaatagt caaaaaagaa aaaccaagtc ttttttgatg acaaaatgca
    23041 atcaaggaaa aattatttta ttttttaacg agatacaaca atcatcttaa cgaagtatat
    23101 aatattatat cgtcaaccta tgctcatatt agattgtttt ttgagcgcac agccgcagct
    23161 aacaccattg caattaacaa atttgcatca atccaccatc aatttgcaca cattattatg
    23221 tgataattgc caaccgctaa atatgcgttt tgttatctat gtataaaaac agcaacttca
    23281 atgtcttaat ggcagttttt cttgatttta atcagcattc atcgccaatt tattgggcat
    23341 attttttcct taagctttag gaatttttta ttatttactt tggggcctgg agacaggaaa
    23401 aattatgctg acattcattg agctccttat tggggttgtg gttattgtgg gtgtagctcg
    23461 ctacatcatt aaagggtatt ccgccactgg tgtgttattt gtcggtggcc tgttattgct
    23521 gattatcagt gccattatgg ggcacaaagt gttaccgtcc agccaggctt caacaggcta
    23581 cagcgccacg gatatcgttg aatacgttaa aatattacta atgagccgcg gcggcgacct
    23641 cggcatgatg attatgatgc tgtgtggatt tgccgcttac atgacccata tcggcgcgaa
    23701 tgatatggtg gtcaagctgg cgtcaaaacc attgcagtat attaactccc cttacctgct
    23761 gatgattgcc gcctattttg tcgcctgtct gatgtctctg gccgtctctt ccgcaaccgg
    23821 tctgggtgtt ttgctgatgg caaccctatt tccggtgatg gtaaacgttg gtatcagtcg
    23881 tggcgcagct gctgccattt gtgcctcccc ggcggcgatt attctcgcac cgacttcagg
    23941 ggatgtggtg ctggcggcgc aagcttccga aatgtcgctg attgacttcg ccttcaaaac
    24001 gacgctgcct atctcaattg ctgcaattat cggcatggcg atcgcccact tcttctggca
    24061 acgttatctg gataaaaaag agcacatctc tcatgaaatg ttagatgtca gtgaaatcac
    24121 caccactgct cctgcgtttt atgccatttt gccgttcacg ccgatcatcg gtgtactgat
    24181 ttttgacggt aaatggggtc cgcaattaca catcatcact attctggtga tttgtatgct
    24241 gattgcctcc attctggagt tcctccgcag ctttaatacc cagaaagttt tctctggtct
    24301 ggaagtggct tatcgcggga tggcagatgc gtttgctaac gtggtgatgc tgctggttgc
    24361 cgctggggta ttcgctcagg ggcttagcac catcggcttt attcaaagtc tgatttctat
    24421 cgctacctcg tttggttcgg cgagtatcat cctgatgctg gtattggtga ttctgaccat
    24481 gctggcggca gtcacgaccg gttcaggcaa tgcgccgttt tatgcgtttg ttgagatgat
    24541 cccgaaactg gcgcactctt ccggcattaa cccggcgtat ttgactatcc cgatgctgca
    24601 ggcgtcaaac ctgggtcgta ccctatcacc cgtttctggc gtagtcgttg cggttgccgg
    24661 gatggcgaaa atctcaccat ttgaagtcgt aaaacgcacc tcggtgccgg tgcttgttgg
    24721 gctagtgatt gttatcgttg ctacagagct gatggtgcca ggaacggcag cagcggtcac
    24781 aggcaagtaa atagtaatgc cggaggcaag tttcctccgg ctttatcatg tcacccactg
    24841 tggtatatgc gttgtggtct gccaactttg ccgtgaacaa tttcagcaat aatcagatgg
    24901 cggctggcgc aatattcaag ataacgcctg gcagtggtgc gactgatggt cagtgcctgc
    24961 gccaccgttt ctgccgtatg ttgcacacca ggctctttaa acagttttcg caccgcgttt
    25021 agcgtcaagg gatcaatgcc ggtcggcagt tcgtctttag gttcaccgcg agcataggca
    25081 ttaaacatct catcaatttg cttctggctg gcgctatcaa tactttccag catatgttta
    25141 cgctggcgga aacgggttag cgtttgcccc agccgttcat aggcaatggg cttaatgaga
    25201 taatcaaata caccacaacg tacggcttca gacaccgttt ccatatcgct ggctgcagtg
    25261 gtaaacacca cgtcgccggg ataatgcgcc tgcaccagtt catgcaataa attaatccct
    25321 ctgccgtcag gaagatagtt atcgagcaag attagccccg gcttaaaacg ctcgatcatc
    25381 attcgggcct gcgccaggtt tcccgccagt aatatctgac tgaatccggg aatgtgacga
    25441 atatattccg catgcatctc tgccagcggc gtttcgtcct caacgatcaa tagggttaat
    25501 ggagctgtca ttaggtttca ctttcggaat atagattgaa aacaaggtac cgcagggatc
    25561 attatcttcg agagtgataa caccaccgca gcgcgttacg tagctggcaa tcaagtacaa
    25621 cccaatgcca tgttcaccgg gctcgtcagc acgcgtactg accccctgct caaatatttt
    25681 gtctcgtaga gactctggaa cgccgcagcc ctgatcggcg acttcaatca ccacatcatc
    25741 gccttcatcg ctgaggaata attcaacgat cttgtttcct tcatcgctac gcaggctggc
    25801 ttcgaaggcg ttatcaagta aattgcccac aatggctgca aactcggtgc tatccagtcc
    25861 tggcggcagt tgcgaaagct ggctaccggg gacaatgatc atttttagcc ccagttcccg
    25921 ggcgcgctgc actttaccaa aaagcagccc cgccacctgg cgatcggcaa acgcctcacg
    25981 caggctgtca ataagctgtt gctgggcctg agactccccc tgcaccatcg ccagcacgcg
    26041 atcatactct ttcatctgca acagaccatt gagcgtcgac atccaattga gatgctcgtg
    26101 tcgcaatgta cgaaggctct cgacgtattg ttttatttgc gtcagttgcg cattgagggt
    26161 ggaaatttcg tctttactac gaaagctgat aatggccccc agcaaatcat cacctgaacg
    26221 aatagcttcc cgattggcaa taacgctcag accgttaaag ttagtcacca catcctgacg
    26281 tttttcatca atctgttcgg taaagaaatc ggcgggcctg accacttcag caatgggttt
    26341 acccaaccat tgccgtccgg gagagctcag ccccagcatc tttcttgcgt tacgattgat
    26401 ggcggtaatg taaccatgcg gatccaccgc aatcagccct tcataaaccg aactaaacag
    26461 cgcctcttgc tgacggacca cgcgtgcgat ttgctttggc tccatgccca tcatttgccg
    26521 acggatatgc gctgccagga accacgacag caacatcaga atccctaaca gcacgacaaa
    26581 cacgcctgcc atcggtaata aaaactcagc ccgccagcta tcgattttac tcaccaggta
    26641 gccaatcgac accacgccga tgacttttcc atcgtcatca aagattggcg ttttggcgcg
    26701 catcgccatc cccattgacc ctttcccggt aatgaagtag ctctcccctt tctccagcgc
    26761 gccctgtttg gtgaactgca taggataacc aattttctcc ggattaggat ggtaaaggcg
    26821 gatcgagtgc cggtccccaa tcaccacata atcaaaatcg gtatctcttt gtaatttgtt
    26881 agcgatggtc gccagccgtt tgtagtcacg cgttttcacc gcagagatga cactgtcatt
    26941 ggaggcaata attttcgcct gattcattgc catgtcgcgt acatgaagcg ttaaatagtc
    27001 ctcaaaactg gccgtaaaat attgcgccag ggctgcaatg acaaatattg agaacaccag
    27061 aatcagcaaa aagatacgca gcggaaatgc cagtctttgg aaaaatgcaa actgtttatt
    27121 ctcgttaagc tgcaacattg ttttccttgc gggttgtccc gagcctcttt gacggatgta
    27181 attaaaatgt tgaatcaaaa tacatagata ttaattatga tcgtggcaaa ttttattcat
    27241 taaatcaatt aaatcactta aacctgcagg tcatttcgta attacattaa ttaaaaaact
    27301 taaaaccatt aaataaataa aaaaccacta actctatgtg aaataaatca aaatttcacg
    27361 cagaaatact ccttaggatg tatagcgaaa ggagaaaaag atatacctcg atcacccccc
    27421 tttctcccaa gtgaaaataa aaggttatca gtttgcaaca ttgaacaaca ttcgttgcaa
    27481 atcgataaca acatgcacct tcaggatact atttattatg ttcggcaatg atattttcac
    27541 ccgcgtaaaa cgttcagaaa ataaaaaaat ggcggaaatc gcccaattcc tgcatgaaaa
    27601 tgatttgagc gttgacacca cagtcgaagt atttattacc gtaacccgcg atgaaaagct
    27661 tatcgcgtgc ggtggaattg ccggaaatat tattaaatgc gttgcgatca gtgaatccgt
    27721 tcgcggtgaa ggactggcgc tgacattagc cactgaactg ataaacctcg cctatgagcg
    27781 gcacagcacg catctgttta tttataccaa aaccgaatac gaggcgctgt tccgccagtg
    27841 cggtttttcc acgctgacca gcgtacccgg cgtgatggtg ctgatggaaa acagcgccac
    27901 gcgactgaaa cgctatgccg aatcgctgaa aaaatttcgt catccaggga acaagattgg
    27961 ctgcattgtg atgaacgcca atcccttcac gaatggtcac cgttatctga ttcaacaagc
    28021 tgcggcacag tgcgactggt tgcatctgtt tttagtcaaa gaagattctt cacgcttccc
    28081 ctatgaagac cggttggatc tggtgttaaa aggcaccgcc gatattccac gcctgactgt
    28141 gcatcgcggc tccgaataca tcatctcccg cgctacgttc ccttgctact tcattaaaga
    28201 acagagcgtc attaaccatt gttacaccga aattgatctg aagattttcc gtcagtacct
    28261 cgctcccgcg ctgggtgtaa ctcaccgctt tgtcggtact gaaccctttt gtcgcgttac
    28321 cgcccagtac aaccaggata tgcgctactg gctggaaacg ccgactatct ccgcaccgcc
    28381 catcgaactg gttgaaattg agcggctgcg ttaccaggag atgccgatat ccgcttcccg
    28441 ggtacgtcaa ctgctggcga aaaacgatct cacggctatc gcgccgctgg tccctgcagt
    28501 cacgctgcat tatttgcaga acctgcttga gcactcccgc caggacgcgg cagctcgtca
    28561 aaagaccccc gcatgagaaa caggtgaaaa atgaaaataa accagcccgc cgttgcaggc
    28621 acccttgagt ctggggatgt gatgatacgc atcgccccac tcgatacgca ggatatcgac
    28681 ctgcaaatca atagcagcgt tgagaaacag tttggcgatg caattcgcac caccattctg
    28741 gacgttctcg cccgctacaa cgtgcgcggc gtacagctga atgtcgatga taaaggcgca
    28801 ctggactgca ttttacgtgc acgactggaa gccctgctgg cacgcgccag cggtatcccg
    28861 gctctgccat gggaggattg ccaatgattt ccgcttcgct gcaacaacgt aaaactcgca
    28921 cccgccgcag catgttgttt gtgcctggtg ccaatgccgc gatggtcagc aactccttca
    28981 tctacccggc tgatgccctg atgtttgacc tcgaagactc cgtagcattg cgtgaaaaag
    29041 acaccgcccg ccgcatggtt taccacgcgc tgcaacatcc gctgtatcgc gatattgaaa
    29101 ccattgtgcg tgtcaacgcg ctggattccg aatggggtgt caacgacctg gaagccgtcg
    29161 ttcgcggtgg tgcggaagtt gtgcgtctgc cgaaaaccga taccgctcag gatgttctgg
    29221 atatcgaaaa agagatcctg cgtatcgaaa aagcctgtgg tcgtgaaccc ggcagcaccg
    29281 gcctgctggc ggcgattgaa tctccgctgg gcattacccg cgcagtggaa atcgctcacg
    29341 cttccgagcg tttgatcggt atcgccctcg gtgcagaaga ctatgtgcgc aacctgcgta
    29401 cagaacgctc cccggaagga actgaactgc tgttcgcacg ctgttccatt ttgcaggccg
    29461 cgcgctctgc gggtattcag gcgttcgata ccgtctattc cgacgctaac aacgaagccg
    29521 gattcctgca agaagccgcc cacatcaaac agctgggctt tgacggcaaa tcgctgatca
    29581 acccgcgtca gattgatctg ctgcacaacc tctacgcacc gacccagaaa gaagtggatc
    29641 acgcccgccg cgtcgtagaa gccgctgaag ccgccgctcg cgaaggcctc ggcgtggttt
    29701 ccctgaacgg caagatggtg gacggtccgg ttatcgatcg cgcccgtctg gtgctctccc
    29761 gtgcagaact ttccggcatc cgcgaagaat aaggcaatca aaatgacgca gaaaattgaa
    29821 caatctcaac gacaagaacg ggtagcggcc tggaatcgtc gcgctgaatg cgatcttgcc
    29881 gctttccaga actcgccaaa gcaaacctac caggctgaaa aagcgcgcga tcgcaaactg
    29941 tgcgccaacc tggaagaggc gattcgtcgc tctggtttac aggacggcat gacggtttcc
    30001 ttccatcacg ctttccgtgg cggtgacctg accgtcaata tggtgatgga cgtcatcgcg
    30061 aagatgggct ttaaaaacct gaccctggcg tccagctccc tgagtgattg ccatgcgccg
    30121 ctggtagaac acattcgcca gggcgtggtt acccgcattt atacctctgg cctacgcggt
    30181 ccactggcgg aagaaatctc ccgcggtctg ctggcggaac cggtgcagat ccactctcac
    30241 ggcggtcgtg tgcatctggt acagagcggc gaactgaata tcgacgtggc tttcctcggc
    30301 gtcccgtcct gtgatgaatt cggtaatgcc aacggctaca ccggtaaagc ctgctgcggc
    30361 tccctcggct atgcaatggt tgatgccgac aacgcaaaac aggttgtgat gcttaccgaa
    30421 gaactgctgc cttatccgca taatccggca agcattgagc aagatcaggt tgatttaatc
    30481 gtcaaagttg atcgcgttgg cgatgctgca aaaatcggcg ctggtgctac ccgtatgacc
    30541 actaacccgc gcgaactgct tattgcccgt agcgctgcgg atgtgattgt caactcaggc
    30601 tacttcaaag aaggtttctc catgcaaacc ggcaccggcg gcgcatcgct ggcggtaacc
    30661 cgtttcctgg aagacaaaat gcgtagccgc gatattcgcg ccgacttcgc cctcggtggt
    30721 attaccgcga cgatggttga tctgcacgaa aaaggtctga tccgcaaact gctggatgtg
    30781 cagagctttg acagccatgc tgcgcaatcg ctggcccgta accccaatca catcgaaatc
    30841 agcgccaacc agtacgctaa ctggggttcg aaaggcgcat cggttgatcg tctcgacgtg
    30901 gtggtactga gcgcgctgga aattgacacc cagttcaacg ttaacgtgct gaccggctct
    30961 gacggcgtac tgcgtggtgc ttccggtggt cactgcgata ccgcgattgc ctctgcgctt
    31021 tccatcatcg tcgcgccgct ggtacgcggt cgtattccga ctctggtgga taacgtgctg
    31081 acctgcatca ccccgggctc cagtgtcgat attctggtca cagaccacgg tatcgcagtt
    31141 aacccggcac gtccggaact ggcagaacgt ctgcaggaag cgggcattaa agtggtttcc
    31201 attgagtggc tgcgcgaacg tgcgcgtctg ctgaccggtg aaccacagcc gattgaattc
    31261 accgaccgcg tcgttgccgt tgtgcgttac cgcgatggct cggtgatcga tgttgtgcat
    31321 caggtgaagg aataagccat gcacctgctt cctgaactcg ccagccacca tgcggtgtca
    31381 attcccgagc tgctcgtcag ccgggatgaa aggcaagcac ggcaacacgt ctggctcaag
    31441 cgccatcctg ttccactggt ctcctttacc gtggttgcgc ctgggccgat taaagacagc
    31501 gaggtcacac gccgaatttt taatcatggc gtgacagcct tgcgtgcctt agccgcaaaa
    31561 cagggctggc aaattcagga gcaggctgca ctggtttccg ccagcgggcc ggagggcatg
    31621 ttgagcattg ccgccccggc tcgcgacctc aagctcgcca ccattgagct tgaacatagt
    31681 catcctctcg ggcggttatg ggatatcgat gtcctgacgc ccgaaggcga aattctctcc
    31741 cgccgcgacg attcactgcc gcctcgccgc tgcctgttgt gcgagcaaag cgcagccgtc
    31801 tgcgcgcgtg gaaaaaccca tcaactgacc gatttactca accgcatgga ggcactgctg
    31861 aacgatgtcg atgcctgcaa cgtcaactaa aaccacaaag cttgcgacgt cattaatcga
    31921 tgagtacgcc ctgctgggct ggcgcgccat gctgactgaa gtcaatctgt caccgaaacc
    31981 aggcctcgtg gatcgcatta actgcggtgc gcacaaagat atggcgctgg aagatttcca
    32041 ccgcagcgcg ctggcgattc agggctggtt accccgtttc attgaatttg gtgcctgtag
    32101 tgcagaaatg gcaccggaag cggtactcca cggattacgc ccaattggta tggcttgcga
    32161 aggtgatatg ttccgcgcca ctgcgggcgt aaacacgcat aaaggcagca ttttttcttt
    32221 agggctgcta tgtgcggcaa ttggccgttt gcttcagctc aaccagccgg taacgccaac
    32281 aaccgtttgt tctacggcgg caagtttctg ccgtggcctg accgatcgcg aactgcgtac
    32341 caataattca caactgacgg caggtcaacg gttgtaccag cagcttggcc ttaccggcgc
    32401 acgcggtgaa gccgaagcgg gttatccact ggtgatcaat cacgccttgc cgcattacct
    32461 cactctgctg gatcaggggt tagatcctga actggcattg ctcgatacct tgctcctact
    32521 gatggcgatc aacggcgata ccaacgttgc atcgcgcggt ggcgaggggg gcctgcgctg
    32581 gctacagcgc gaggcgcaaa cattattgca aaaagggggc attcgaaccc ccgccgatct
    32641 cgattatctc cggcagttcg acagggagtg tatcgaacga aatctcagtc caggcggcag
    32701 tgccgacctg ctgatcctta cctggttttt agcacagatt taattattta agcacttgat
    32761 aaatttggaa atattaattt tcggagaacc cgtatgtctt tagcaaaaga taatatatgg
    32821 aaactattgg ccccactggt ggtgatgggt gtcatgtttc ttatccctgt ccccgacggt
    32881 atgccaccac aggcatggca ttacttcgct gtgtttgtgg caatgattgt cggcatgatc
    32941 ctcgagccaa ttccggcaac agcgatcagt tttattgcgg ttactatttg cgttattggc
    33001 agtaattacc tgctctttga tgccaaagaa ttagctgacc cagcgtttaa tgcgcaaaaa
    33061 caggcgctga aatggggtct ggctggtttt tccagcacca cggtatggct ggtatttggc
    33121 gcatttattt ttgcattagg gtatgaagtt tccgggttag gtcgtcgcat tgcccttttc
    33181 ctggtgaaat tcatgggcaa acgcacgctg acgttgggtt atgcgattgt cattatcgac
    33241 attctgctgg caccgtttac accgtccaac accgcgcgta ccgggggtac ggtttttccg
    33301 gtcattaaaa acctgccgcc gctgtttaaa tcattcccca acgatccgtc cgcgcgtcgt
    33361 attggcggct atttgatgtg gatgatggtc attagtacca gtctgagttc gtccatgttt
    33421 gtcaccggtg cggcaccaaa cgtgctgggt ctggagttcg tcagcaaaat tgccggtatc
    33481 cagattagct ggttgcagtg gttcctctgc ttcctgccgg ttggggttat cttgcttatc
    33541 attgcgccgt ggctttccta cgtgctgtac aaaccggaaa tcacacacag tgaagaagtg
    33601 gcaacctggg cgggtgatga actaaaaacc atgggtgcgc tgacacgcag agagtggacg
    33661 ctgattggcc ttgtattgct cagcttaggt ttgtgggtat ttggcagtga agtcattaat
    33721 gctactgcgg ttggtctgct ggcagtttcg ctaatgctgg ccctgcacgt tgtgccttgg
    33781 aaagatatta cccgctataa cagcgcatgg aacacgctgg tcaacctggc aactctggtt
    33841 gtgatggcta acggcctgac tcgttctggc tttattgact ggttcgccgg taccatgagt
    33901 acgcacctgg aaggattctc accaaacgca acggtgattg tactggttct ggtgttctac
    33961 tttgcacact acctgtttgc cagcctgtct gcgcacaccg caaccatgct gccggttatt
    34021 ctggccgtcg gtaaaggtat tccgggcgta ccaatggaac aactgtgtat cctgctggtg
    34081 ctgtctatcg gtatcatggg ctgtctgacg ccgtatgcaa ccggtcctgg ggtgattatt
    34141 tacggctgtg gctatgtgaa atcaaaagat tactggcgtc ttggcgcaat cttcggggtg
    34201 atttacatct ctatgttgct gttggttggc tggccgattc tcgccatgtg gaactaatac
    34261 gttcgccaaa cgctgaataa atcatcacgc ccgccaggta agcccacctg gcgggctttt
    34321 ttatgattta atagatagta ttcagtgtat gtatgagttc cacacccatt atgaaagcat
    34381 tctggcgtaa cgccgcgttg ctcgcggttt ctctgcttcc cttctcttct gccaacgcct
    34441 tagcgttgca ggcaaaacag tatggcgatt ttgatcgcta tgtcctggcc ctctcctggc
    34501 aaaccggatt ttgccagagt caacacgatc gaaatcgtaa cgaacgagat gaatgtcgcc
    34561 tgcaaaccga aacgaccaac aaagctgatt ttctgaccgt acatggtctg tggccaggat
    34621 tgcctaaatc ggttgctgcc cgtggtgttg atgaacgccg ctggatgcgc ttcggttgcg
    34681 ctactcgccc aatcccgaat ctaccagaag cgcacgccag ccggatgtgt tcatcgccgg
    34741 aaaccggatt atcactggaa acggccgcta aactaagtga agtcatgcca ggagctggcg
    34801 gacgttcctg cctggaacgc tacgaatatg ccaaacacgg tgcctgcttt ggttttgatc
    34861 cggacgcata cttcggtacg atggtacgcc tgaatcaaga aattaaagag agcgaagccg
    34921 gaaaattcct tgcggataat tacggtaaaa cagtgagccg ccgtgacttt gacgccgcct
    34981 ttgccaaaag ctggggaaaa gagaacgtga aagcagttaa gctaacgtgc cagggtaacc
    35041 ctgcgtattt gactgaaatt cagatctcga tcaaagctga cgccatcaac gctccgcttt
    35101 ctgcaaactc attcttgcca caaccacacc caggcaactg tggcaaaacc tttgtgattg
    35161 ataaagtggg ttattaatca tctgatttac aaaacacgct aattcgtctt ccataaaccg
    35221 tgacgattaa gaattataca taatgcgctg cattgaggaa ttccctgaat gtgacgcaaa
    35281 tcacttcaaa gagtgagcaa cagtcagtat catcatgtga gttaaaccct cgccgcctga
    35341 cggtgagggt tttcttttgg gtatgtttat ctgcgacact cgcagtaccg acgacatgga
    35401 gtaaaaatgt ccagaccaac tatcatcatt aacgacctgg atgccgaacg catcgatatt
    35461 ctgctggagc aacccgccta tgctggtttg ccaatcgccg acgcgttaaa cgcagagttg
    35521 gatcgcgccc aaatgtgttc gccagaagag atgccacacg acgtggtgac aatgaacagc
    35581 cgggttaaat tccgcaatct tagcgatggc gaagtgcgtg tgcgcacgct ggtgtatccg
    35641 gcaaaaatga ccgatagcaa tactcagctt tccgttatgg ctccggtagg tgccgcactg
    35701 ctggggctgc gcgttggcga ttccattcac tgggaacttc cgggcggcgt tgcaacccac
    35761 cttgaagtgc tggaactcga ataccagcca gaagctgctg gcgactacct gctttaatcc
    35821 catctgcaca agcgataact cgctttgccc agaggatgca ctcgcatcct ctttacgcaa
    35881 aatcagcaat ttttcctggt tctcactgtt cacttttgct gcaaaattat tccgtttcgt
    35941 ttgtgggaat aaccatggca gaagcatttt acatcctgat aggatttctg attatggcgg
    36001 cgattatcgt catggctgtc ctttacctcg aaaaccactc ctgacgatca tattgttccc
    36061 cccggcatcg cattcaccag acctgaaacc gccttctgcg ccgcctctgc gctttgtgca
    36121 ccgggtacca gaatcacctt acggcactcc tcttcacgtt tttcgaaaat ctcatatccc
    36181 cgggcggcct cttcaaacgg catatagtgg gtaacaattt cttctggttt cagtaatcct
    36241 ttctcaatta acggtaataa ttctcccagc catgcgtgaa cgtgggtctg tcccatttta
    36301 aacgtcaacc ctttatcaaa ggcgtcgcca aacaggaaac cgtgaataaa tccagcgtag
    36361 acgcccggta cgctaacaat gccgccacgc ctgaccgccg caatacactg gcgcaacgct
    36421 ttaccgctgc tgccctccag tttcaggtta gtcagcaccg tttccgtggt gctgcctttc
    36481 gcttcaaaac cgacggcgtc tattactgca tccacgcccc ggtgacctgc cgtttgttca
    36541 ataattgact gtgccggatc gctatcttca tcgaaattaa tcgggatcgc gccgtagcgg
    36601 tcagcggcga aacgcaagcg gtagggatga tgatcaacaa caaaaatctg ttccgcaccg
    36661 agcaaccgtg cacaggcgat tgtcaacaat cccactggac cggcaccata gactgcaacg
    36721 cttgaacctt gttggatctg cgcatttttt gctgcctgcc atgccgttgg cagaatatca
    36781 gaaaggaaaa gggctttatc atctgaaagc aaaggcggta ctttaaacgg ccccacattc
    36841 cctttaggga cgcggacata ttccgcctgt ccaccaggaa cgccaccata caggtgacta
    36901 taaccaaaca atgccgcggg agctggtatc tgttttttat tgagcgcagc gcctttaccc
    36961 gcattggtat tttcgcaggc ggcatattgc tgcattcgac agaaaaaaca gtcgccacaa
    37021 gcaatgacaa acggaattac cacccggtcg cctttttgca aattttttac gtcctttccg
    37081 gtttcaacga cttcccccat aaattcatga ccaaaaatat cgccatgttt aacctggggt
    37141 attttgcctc gataaagatg gaggtcagag ccacagatcg ccgttgccgt aatacgcaga
    37201 ataatatcat ctgcctgttc aatgcccgga tcgggaacat tttctacctg aacgtgatgt
    37261 gggccgtgat aagtcaatgc tttcatgcaa cctccagaga cagttcaaaa aagagtcagt
    37321 ctttaagggt agctatcaca gcgggaaaac gggcggtacc gcaggttttg ggagaattcc
    37381 tggtgttcag tgagaatata ttaatcacag aaaccgttcg taaggaaaaa gaaccagaca
    37441 aatatcaggg ataaatatta taatcatggc gaatgatttg ttcatgatta acagggagaa
    37501 agggttatgt ataagacaat cattatgcca gttgatgtat ttgaaatgga attgagcgac
    37561 aaagctgttc gccacgctga attcctcgcc caggatgacg gagttattca tctacttcac
    37621 gtactacccg ggtcagccag cctgagcctg caccgttttg ccgctgatgt gcgtcgtttt
    37681 gaagagcatc tgcaacatga agcagaagaa cgtctgcaaa cgatggtcag ccacttcacc
    37741 atcgatcctt cccgcattaa acaacatgtc cgttttggta gcgtgcggga tgaagtcaat
    37801 gagttggcag aagaactggg ggctgatgtt gtagttattg gttctcgcaa cccatcgatt
    37861 tcgacccatc tgttaggttc taacgcctcg agcgtgatcc gccatgccaa tctgccggtg
    37921 ctggttgtgc gttaattgcc agaataaata tcccgccctg cccggagcca tcccggcagg
    37981 gctttttaat gccataaaaa agccgccagg tttgaccccg gcggctaagc aattgcaggt
    38041 gaatcttact tcttcttatg cagttttggt gcgaatcagg tagtcaaaag cactcagaga
    38101 ggctttggca ccttcgccag tggcgatgat aatctgcttg tacggaaccg tcgtacagtc
    38161 acccgctgcg aacacgcctt tgacgttggt ttcgcatttc gcatcaatga taatctcacc
    38221 catgcggtta cgttcgactg cgccttccag ccagttggtg ttcggcagca gaccaatctg
    38281 gacgaaaata ccggccagtt cgatgttgtg aatatcgccg ctgacacgat ctcgatattc
    38341 cagaccaacg actttgctgc cgtcgccttt cacttccgtg gtttgcgcat tcagaataat
    38401 gtcgacgttt ttcaggctgc gcagtttgtc ctgcagaacc tggtcggctt tcatttctgg
    38461 cgcaaattcg agcagcgtta cgtgctcaac gatacccgcc aggtcgattg ccgcttccac
    38521 gccggagtta ccgccgccga taaccgctac gcgtttgcct ttaaacagcg ggccgtcgca
    38581 gtgcgggcag taggtcacgc ctttggtgcg atactgatct tcgcccggaa cgttcatgtt
    38641 gcgccatttt gcaccggtcg ccacgataat gctgcgtgct ttcagtaccg cgccagaagc
    38701 tgtttcaatc tggtgtagac caccttcaac cgctgccggg atcagtttgc tggcgctctg
    38761 gctgtcgatc acatcaacgt cgtattcatc aacgtgaact ttcagtgcgc ctgccagttt
    38821 ctgcccttca gtcttcggta cagaaatgta gttttcgata tcaacggtat cgaggatctg
    38881 accaccaaaa cgttcgccca tcagaccggt acggatgcct ttacgtgcgg agtaaattgc
    38941 tgccgctgca cccgccgggc cggaaccgac gattaatacg tcataagcat cacgcttgtt
    39001 cagctcttct gccgcacgtt tttccgcgcc agtatcaatt ttggcaacga tttcagtcaa
    39061 cgtcatgcgg ccctgaccaa actctttccc gtttacgaac actgccggaa cgcccatcac
    39121 gttgcgatcg gtgatttcgt tctggaaggt gccgccgtca attgcagtgt gcttgatgcg
    39181 cgggttcaat acgctcatca ggttcagcgc ctgcaccacg tccgggcagt tatggcaaga
    39241 gagcgagtaa taggtttcga attcaaaatc accgtcaata tggcgaatct gctccagcag
    39301 agactgcgct tctttcgacg gatgaccacc ggtccacagt aacgccagta ccagcgaggt
    39361 gaactcgtgg cccagcgggg agcctgcaaa acgtggcccc tggttggaac ctgggttggt
    39421 gatcaggaaa gacggcttac gcaccggcaa gctgttatct tctttaaagg tgactttgtc
    39481 tgacagttct gcgatttcag ccaacagttc cttgatttct gccgatttag cgctgtcatc
    39541 cagcgtggca attaactcaa caggcttggt caatttctca aggtaagcct tgagttgagt
    39601 tttcatattt gtgtcgagca tgtttatctc ctgggcttta aacatcatca tgcaagcggc
    39661 ctctgcggct acctggatgc agcttgcatc atggtgcggg gaagaaaatc gggtgccgca
    39721 gcacccgaag aattaggtga atttccgaag tatttcgcgc cggagttggt agcgcgaaag
    39781 acgaaggaaa tttagatttt accaaccaag tccagagacg gagccagagt tgcttcacct
    39841 tctttccatt tagccgggca aacttcacct gggtgagaag ctacgtactg tgctgctttg
    39901 attttacgca gcaggtcaga cgcgtcacgg ccaatgcctt cagcggtaac ttcgattgcc
    39961 tggatgatac cctgcgggtc aacaacgaag gtcgcacgat cagccagacc ttcatcttca
    40021 cgcatgttgt cgaagttacg ggtcagggcg ccagtcgggt cgccgatcat cgcatatttg
    40081 attttagcga tggtttcaga gctgctgtgc catgctttgt gggtgaagtg agtatcggta
    40141 gatactgcgt atacgtctac gcccagtttc tgcagttctt cgtagtggtc agcaacgtca
    40201 cccagttcgg tcgggcatac gaaagtaaag tcagccgggt agaagaagaa gacgctccag
    40261 cggccttctg tatctttttc ggtgatttcg atgaattcgc cgtttttgaa tgcctggttt
    40321 ttaaaaggtt taattttggt gttaatcaag gacatctata cttcctccgt gttttcgatg
    40381 agatgtaagg taaccaattt ttgccgattc ggctaatgcg tttccattat caaatcgata
    40441 agttttacct tacaaccttc gtaagacaac tttgtgaact ttgcaccgcc agcggcgata
    40501 aaaagtaaaa tggccccctt tccaggtgac cgtgtaatct gaactaccct ttggtatctt
    40561 cagcgactgc ctgcaggctg agtgttgccc ttacaaatgc aacaacgaca tggattacaa
    40621 caccctcata aacaaagggc aatcacctga tctaagctct tacctatgac agtgataggt
    40681 tatgcctttt actcgacttt tgcactgact gaaaaggaca aattaatgtt aaaaaagata
    40741 cttttactgg ctctgcttcc tgcaatcgcc ttcgcagagg aacttcctgc tccagtaaaa
    40801 gcgattgaaa aacagggcat tacaatcatc aaaacgttcg atgcccccgg aggaatgaaa
    40861 ggttatctcg gaaagtatca ggatatgggc gtcaccatct acctgactcc agatggtaag
    40921 cacgctatct ctggttacat gtacaacgag aaaggtgaaa acctgagtaa cacacttatc
    40981 gaaaaagaaa tttacgcacc agccggacgc gaaatgtggc aacggatgga acaatcccac
    41041 tggctcctcg acggtaaaaa agatgcgccg gtcattgtct acgtcttcgc cgatccgttc
    41101 tgcccatatt gtaaacagtt ctggcagcag gcgcgcccgt gggtagattc tggcaaagtg
    41161 caattaagaa cattgttggt tggggttatc aagccagaaa gcccggcgac agcagcggca
    41221 attcttgcct ccaaagatcc cgcaaaaacc tggcaacaat atgaagcctc cggtggcaag
    41281 cttaagctaa acgtgcctgc aaacgtaagt acagagcaaa tgaaagtgtt aagtgacaat
    41341 gagaaactga tggacgatct gggggcaaat gtcacgccgg ctatctatta catgagtaag
    41401 gaaaatacgc tacaacaggc cgtggggttg cccgatcaga aaacgcttaa tatcattatg
    41461 gggaataaat aaaagataat tcatggatgg ttgaaataca tctctgcttt tcaaccatcc
    41521 taaaaaagca tga
//
LOCUS       DAJOWB010000027        41323 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJOWB010000027 DAJOWB010000000
VERSION     DAJOWB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.027"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(40..321)
                     /locus_tag="OHO85_001654"
     CDS             complement(40..321)
                     /locus_tag="OHO85_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCQ2671037.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(584..1693)
                     /gene="apbC"
                     /locus_tag="OHO85_001655"
     CDS             complement(584..1693)
                     /gene="apbC"
                     /locus_tag="OHO85_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCQ2671038.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1825..3858
                     /gene="metG"
                     /locus_tag="OHO85_001656"
     CDS             1825..3858
                     /gene="metG"
                     /locus_tag="OHO85_001656"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCQ2671039.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3999..7793
                     /locus_tag="OHO85_001657"
     CDS             3999..7793
                     /locus_tag="OHO85_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HCQ2671040.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQDNELHISWGKIGTHGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIEWDYANNVIIILPSGIAPSYLEQYSRFELR
                     LRKHLSLTEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKAIAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCSADSLAGFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDETRANDAVNRYKLLKKDARTIAAQQVAR
                     LESAMCLRRRWSLENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWIKGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            7803..11435
                     /locus_tag="OHO85_001658"
     CDS             7803..11435
                     /locus_tag="OHO85_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HCQ2671041.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRKWPGEPVPVITESIIQ
                     TLGDKLLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPARTMAALVCIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFITRCYESDPLLVTLQYQDEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESSWQRCADKLIAALPGIPKIRRPFIALLLPEKPEIANELASL
                     ESSRSSLHSKEWLKVIATDNTAVKKLERYWGLDVFSDREASYMSQENRFGYAACASLL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLMASQPQLAAE
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHTLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIIPPLRQLTRRTVLLTPDESASNSLNRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYSDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11496..11813
                     /locus_tag="OHO85_001659"
     CDS             11496..11813
                     /locus_tag="OHO85_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2671042.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     EIE"
     gene            12120..13208
                     /locus_tag="OHO85_001660"
     CDS             12120..13208
                     /locus_tag="OHO85_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCQ2671043.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            13219..15498
                     /locus_tag="OHO85_001661"
     CDS             13219..15498
                     /locus_tag="OHO85_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2671044.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEVDDSPDTQEESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRLREAFMARWIGWAMQQNNGDVLVVCGGWHAPVLAKMWRECPQEIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            15491..16627
                     /locus_tag="OHO85_001662"
     CDS             15491..16627
                     /locus_tag="OHO85_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCQ2671045.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAMEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            16624..18624
                     /locus_tag="OHO85_001663"
     CDS             16624..18624
                     /locus_tag="OHO85_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCQ2671046.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRTLQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMSCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            18749..19210
                     /locus_tag="OHO85_001664"
     CDS             18749..19210
                     /locus_tag="OHO85_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCQ2671047.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(19251..19721)
                     /locus_tag="OHO85_001665"
     CDS             complement(19251..19721)
                     /locus_tag="OHO85_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCQ2671048.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(19768..20487)
                     /gene="yehT"
                     /locus_tag="OHO85_001666"
     CDS             complement(19768..20487)
                     /gene="yehT"
                     /locus_tag="OHO85_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCQ2671049.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(20484..22169)
                     /gene="btsS"
                     /locus_tag="OHO85_001667"
     CDS             complement(20484..22169)
                     /gene="btsS"
                     /locus_tag="OHO85_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCQ2671050.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            22391..23122
                     /gene="mlrA"
                     /locus_tag="OHO85_001668"
     CDS             22391..23122
                     /gene="mlrA"
                     /locus_tag="OHO85_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCQ2671051.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            23182..23289
                     /locus_tag="OHO85_001669"
     CDS             23182..23289
                     /locus_tag="OHO85_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCQ2671052.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(23270..24001)
                     /gene="yehW"
                     /locus_tag="OHO85_001670"
     CDS             complement(23270..24001)
                     /gene="yehW"
                     /locus_tag="OHO85_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCQ2671053.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(24006..24932)
                     /gene="yehX"
                     /locus_tag="OHO85_001671"
     CDS             complement(24006..24932)
                     /gene="yehX"
                     /locus_tag="OHO85_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCQ2671054.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(24925..26082)
                     /gene="yehY"
                     /locus_tag="OHO85_001672"
     CDS             complement(24925..26082)
                     /gene="yehY"
                     /locus_tag="OHO85_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCQ2671055.1"
                     /translation="MTYLRINPVLALLLLLTVIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSIFALILVQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(26089..27006)
                     /gene="osmF"
                     /locus_tag="OHO85_001673"
     CDS             complement(26089..27006)
                     /gene="osmF"
                     /locus_tag="OHO85_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCQ2671056.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(27217..29514)
                     /gene="bglX"
                     /locus_tag="OHO85_001674"
     CDS             complement(27217..29514)
                     /gene="bglX"
                     /locus_tag="OHO85_001674"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCQ2671057.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            29710..31425
                     /gene="dld"
                     /locus_tag="OHO85_001675"
     CDS             29710..31425
                     /gene="dld"
                     /locus_tag="OHO85_001675"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCQ2671058.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(31463..32395)
                     /gene="pbpG"
                     /locus_tag="OHO85_001676"
     CDS             complement(31463..32395)
                     /gene="pbpG"
                     /locus_tag="OHO85_001676"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCQ2671059.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKAIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(32569..33156)
                     /locus_tag="OHO85_001677"
     CDS             complement(32569..33156)
                     /locus_tag="OHO85_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCQ2671060.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            33326..33904
                     /locus_tag="OHO85_001678"
     CDS             33326..33904
                     /locus_tag="OHO85_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCQ2671061.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(34034..34795)
                     /locus_tag="OHO85_001679"
     CDS             complement(34034..34795)
                     /locus_tag="OHO85_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCQ2671062.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(34848..36284)
                     /gene="mdtQ"
                     /locus_tag="OHO85_001680"
     CDS             complement(34848..36284)
                     /gene="mdtQ"
                     /locus_tag="OHO85_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCQ2671063.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            36508..36591
                     /gene="yohP"
                     /locus_tag="OHO85_001681"
     CDS             36508..36591
                     /gene="yohP"
                     /locus_tag="OHO85_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCQ2671064.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(36962..37909)
                     /gene="dusC"
                     /locus_tag="OHO85_001682"
     CDS             complement(36962..37909)
                     /gene="dusC"
                     /locus_tag="OHO85_001682"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCQ2671065.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            38148..38546
                     /locus_tag="OHO85_001683"
     CDS             38148..38546
                     /locus_tag="OHO85_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCQ2671066.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            38543..39238
                     /locus_tag="OHO85_001684"
     CDS             38543..39238
                     /locus_tag="OHO85_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCQ2671067.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            39368..40252
                     /gene="cdd"
                     /locus_tag="OHO85_001685"
     CDS             39368..40252
                     /gene="cdd"
                     /locus_tag="OHO85_001685"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCQ2671068.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVTLECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            40402..41121
                     /gene="sanA"
                     /locus_tag="OHO85_001686"
     CDS             40402..41121
                     /gene="sanA"
                     /locus_tag="OHO85_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCQ2671069.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            41124..>41323
                     /locus_tag="OHO85_001687"
     CDS             41124..>41323
                     /locus_tag="OHO85_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCQ2671070.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGI"
BASE COUNT        10055 a        10998 c        10450 g         9820 t
ORIGIN      
        1 tttcggcttt ctaaatgcaa ttaaaatcag gttgccaaat taatcgtagc gatttaacac
       61 gattcgacgt ttgctatcgc tacctgcaac aaccacttct gtcgtaacac ctttgacggg
      121 cgaagaaacc tgatgacggg tgtcaacatt atacgttaac tgaccacaat gctgttgtgg
      181 ttgactatta accatagcca tactgcatgt tgggggactg atcctgccat taatcgttaa
      241 tgtggcagtc tctgaagaga atgcgggaga agccaatcca taggcaagaa aaataattcc
      301 tgacagccaa tacttattca ttgaacgtta tccctgtagt aaaggttatg cctggataga
      361 atgagtgcat aacaaactat agctgtacat ccactacaca gccacgaagg atgataatga
      421 agcattgcct gtatgatcaa tcgactttgt agagtttcgg acgaaggtcc gcagaatatt
      481 cgcagtatta aataagtgtt cattaatgcg gcaaaaacta atacaccgca tcaatgtaac
      541 atctctgcgg tgtatgaaga ataaccagaa tggttaatag gcgttagacc gcgcggaagg
      601 aaatctcgcc tggaatgact tcaccctgcc agtagagctg agctgcaacg cggtcagcca
      661 gctggcggta gatagcggta aattcgctct ccggacggct gataaccgtt ggcgtgcctt
      721 tatcgagatc ctcacgtaag gaaatgtgta acggcatctg ccccagcagc tgtgtgtggt
      781 atttttcagc cagtttctct gcaccaccgg taccgaaaat cggttcatgg tgaccacagt
      841 tactgcaaat atgcacactc atgttctcga caataccaag caccggaacc tcgactttct
      901 cgaacatcac aatgcctttt ttcgcatcga tcaacgcgat gtcctgcggg gtggtgacca
      961 cgactgcacc agttactgga atgttttgcg ccagcgtcaa ttgaatgtca ccggtgcctg
     1021 gcggcatatc aagcaccaga tagtcgagat ccggccacaa ggtttcctgc aacatctgca
     1081 ttaacgcctt gctcgccatc ggtccacgcc acaccatcgc attgtcgtca gtgaccagat
     1141 aaccaattga attggttgcc aggccatgag acataatcgg tgccatatgg gtaccgtccg
     1201 gcgaagttgg acgctgattt tctgcgccca gcatggtggg aattgatggg ccgtagatat
     1261 cggcgtccag aatgccgact ttcgcccctt ccgccgccag tgccagcgcc aggtttaccg
     1321 ccgtggagga tttacccacg ccgcctttac cggaactgac cgcaatgatg ttcttcacac
     1381 cattaatacc cggctgattc ttcacgcgtt ttaaggtggc gatattgtgc gacagtttcc
     1441 agtcgatagc tttcgccccg gtgatacgca gcagttcggc gctacattgc tcttttaact
     1501 cttcaaacgc gctatgccag acgaatggca taaccagttc cacatgcaac gtgtcgtcca
     1561 tccaggcgac atggtgcaac gctttcagcg tggtgaggtt atgcttcagg gttgggtgct
     1621 gaaaattggc cagtgtcccg gcgaccattg cgcgcaaggc ttctggtgat ttggcctggg
     1681 attgttcgtt catcccgact ccttttttgt atagataaac catcagctga tagtttacct
     1741 gaagaatata gagaagtact tacttaacat tttcccattt ggtactatct aacccctttt
     1801 cactattaag aagtaatgcc tactatgact caagtcgcga agaaaattct ggtgacgtgc
     1861 gcactgccgt acgctaacgg ctcaatccac ctcggccata tgctggagca catccaggct
     1921 gatgtctggg tccgttacca gcgaatgcgc ggccacgagg tcaacttcat ctgcgccgac
     1981 gatgcccacg gtacaccgat catgctgaaa gctcagcagc ttggtatcac cccggagcag
     2041 atgattggcg aaatgagtca ggagcatcag actgatttcg caggctttaa catcagctat
     2101 gacaactatc actcgacgca cagcgaagag aaccgccagt tgtcagaact tatctactct
     2161 cgcctgaaag agaacggttt tattaaaaac cgcaccatct ctcagctgta cgatccggaa
     2221 aaaggcatgt tcctgccgga ccgttttgtg aaaggcacct gcccgaaatg taaatccccg
     2281 gatcaatacg gcgataactg cgaagtctgc ggcgcgacct acagcccgac tgaattgatc
     2341 gagccgaaat cggtggtttc tggcgctacg ccggtaatgc gtgattctga acacttcttc
     2401 tttgatctgc cctctttcag cgaaatgttg caggcatgga cccgcagcgg tgcgttgcag
     2461 gagcaggtgg caaataaaat gcaggagtgg tttgaatccg gcctgcaaca gtgggatatc
     2521 tcccgcgacg cgccatactt tggttttgaa attccgaacg cgccgggcaa atatttctac
     2581 gtctggctgg acgcaccgat tggctacatg ggttctttca agaatctgtg cgacaagcgc
     2641 ggcgacagcg taagcttcga tgaatactgg aagaaagact ccaccgccga gctgtaccac
     2701 ttcatcggta aagatattgt ttacttccac agcctgttct ggcctgccat gctggaaggc
     2761 agcaacttcc gcaagccgac caacctgttt gttcatggct atgtgacggt gaacggcgca
     2821 aagatgtcca agtctcgcgg cacctttatt aaagccagca cctggctgaa tcattttgac
     2881 gctgacagcc tgcgttacta ctacactgcg aaactctctt cgcgcattga tgatatcgat
     2941 ctcaacctgg aggatttcgt tcagcgtgtg aatgccgata tcgtgaacaa agtggttaac
     3001 ctggcctctc gtaatgcggg ctttatcaac aagcgttttg acggcgtgct ggcaagcgaa
     3061 ctggctgacc cgcagttgta caaaaccttc actgatgccg ctgaagtgat tggtgaagcg
     3121 tgggaaagcc gcgaatttgg taaagcagtg cgcgaaatca tggcgctggc tgatctggct
     3181 aaccgctatg tcgacgagca ggctccgtgg gtggtggcaa aacaggaagg ccgcgatgcc
     3241 gacctgcagg caatttgctc aatgggcata aacctgttcc gcgtgctgat gacttacctg
     3301 aagccggtac tgccgaaact gaccgagcgc gcagaagcat tcctcaatac agaactgacc
     3361 tgggatggta tccagcaacc gctgctgggc cacaaagtga atccgttcaa agcgctgtat
     3421 aaccgcatcg atatgaagca ggttgaagca ctggtggaag cgtctaaaga agaagtgaaa
     3481 gcagctgccg cgccagttac tggcccactg gcagatgatc cgattcagga aaccatcacc
     3541 tttgacgact tcgcaaaagt tgacctgcgc gtggcgctga tcgaaaacgc agagtttgtt
     3601 gaaggttctg acaaactgct gcgcctgaca ctggatctcg gcggtgaaaa acgcaatgtc
     3661 ttctccggta ttcgttctgc ctacccggat ccgcaggcac tgatcgggcg tcacaccatt
     3721 atggtggcca acctggcacc gcgtaaaatg cgctttggta tctccgaagg catggtaatg
     3781 gctgctggtc ctggcgggaa agatatcttc ctgttaagcc cggatgctgg tgctaaacca
     3841 ggccatcagg tgaaataatc cccctttaag gcgctgcatc gacagcgcct tttctttata
     3901 aattcctaaa gttgttttct tgcgattttg tctctctcta acccgcataa atactggtag
     3961 catctgcatt caactggata aaattacagg gatgcagaat gagacacttt atctatcagg
     4021 acgaaaaatc acataaattc tgggcagttg agcaacagga taatgagctt catattagct
     4081 ggggcaaaat cggtacccac ggacaaagtc agataaaaag tttttcagat gctgcggcag
     4141 cggcaaaagc ggagcttaag ctgattgcgg agaaggtgaa gaaggggtat gtggagcaag
     4201 cgaaggataa ttctttgcaa ccttcccaaa cggtaacggg ctctctcaag gtagcggact
     4261 tatccaccat tattcaggaa caaccctctt tcgtagcaga aacccgtgcg cctgacaaaa
     4321 atacagatgc tgttttaccg tggctggcga aagatattgc tgtcgttttt ccgcccgaag
     4381 ttgtacacac cacgttaagt catcgccgct ttcccggagt tcctgttcag caagcagaca
     4441 aattgaccca attacgtcgc ttagcctgta gtgtgtcgca acgggataat aaaacagcca
     4501 catttgactt cagcgcctgt tctttagaat ggcaaaacac cgtcgcccag gcgatcagtc
     4561 agatcgacgg cctgaaaaca acacagttac catcaccagt aatggctgta ctcacggcac
     4621 ttgaaatgaa atgcacaaga tataaagtgc gtgaggatgt tatggatcag atcgtccagg
     4681 aaggcggtct ggaatatgct actgatgtaa taatacacct tcaacagatt gatattgaat
     4741 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct agctacttgg
     4801 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactgacg gaagagtctc
     4861 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
     4921 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
     4981 gtctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
     5041 agcacattct tgcctcatta gaaaaatatc acgaaccata tgccattttt gatgattact
     5101 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
     5161 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
     5221 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgga
     5281 tgacgaaagc cattgctgcg ttcccacatg cagcaatggc ggcactgacg gaacttcttg
     5341 ggcaaaaaga agagaacagt tggcgcatta tgctaatgac aatgcttatc tcacaaccag
     5401 cactggcaga acaggtcatt ccctggctct cgacacccgc agttgccgta ctgaaatcat
     5461 gccagcaaca actgacacag ccctcaaacc atgccagcgc cgatctactg ccagccgtag
     5521 tagtctcccc tccctggctt tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag
     5581 cgccattagg cattgagcca atctgttatc tgacagaaga aatcagtaat caacttttgg
     5641 cgaaatatat ctggtattca aaacacatca cggttagcca tgaagaaagt actaccaacc
     5701 tgttggcaag gatgggtttt caacgacgga tcgctggtac atatattaaa gctcccgaag
     5761 cggtagttga ggcatggcta aatgaagatt attcaacctt actaagtgaa tttaaggtgt
     5821 ttcattcacc taccgggcat tattggcagt tggggatttt gacaacattg ccgctggaga
     5881 aagcagtaaa agcatggaat gcccttaccc tatctccaca taccgatacc gaatacgcca
     5941 tgttacattt tggactcaaa gggttacctg ggttagtaaa ctcacttgca cgctatccac
     6001 aagaagcctt gcccatcacg aattacttcg cagcgagtga gctggctcct gccgtcgccc
     6061 gtgccttcaa caaactgaaa acgctacgcg aaaacgcccg tagctggctg ttgaaatacc
     6121 cggaacatgc ccttaccggc ctgctgcctg cggcgctcgg caaagccggt gaagcacagg
     6181 ataacgcccg cgctgccttg cgtatgctta ccgaaaacgg tcatcagcca ttactgcaag
     6241 aaatcgcccg acgttataac cagccggaag taaccgatgc ggtgaacgct ctgcttgcgc
     6301 tcgatccctt agataatcac ccgacaaaaa tccccactct tccggccttt tatcagccat
     6361 cgctctggac gcgcccggta ttaaaagcaa atgcccaatc actgccagat agcgccctcc
     6421 tccacctcgg tgaaatgctc cgcttccctc aggaagaggc tctgtatccg ggattattgc
     6481 aggtgaaaga cgtctgttcc gccgactcac tggcgggatt tgcctgggat ctgtttaccg
     6541 cctggcagac cgctggcgcg ccgtcgaaag agagttgggc gttcactgcg ttaggcgttc
     6601 tcggtaacga tgacaccgcc cgcaaactga cgccattaat acgcgcctgg cctggtgaat
     6661 cccagcataa acgcgccacc gttgggttgg atattctcgc tgctatcggt agtgatatcg
     6721 cccttatgca gcttaacggc atcgcccaga aactgaaatt caaagcatta caggagcggg
     6781 caaaagaaaa aattgccgac attgccgaga gccgcgaact cacggtggcg gagcttgaag
     6841 atcggttagc accggatctc ggtctggatg ataacggttc gctgctgctg gattttggcc
     6901 cacggcagtt caccgtcagc tttgatgaaa ccttaaaacc gtttgtgcgt gatgtttccg
     6961 gcagccgcct gaaagacctg cccaaaccga acaaaagcga tgatgaaacg cgggcgaacg
     7021 atgcggttaa ccgctacaaa ttgctgaaaa aagatgcgcg taccatcgcc gcccagcagg
     7081 tagcaaggct ggaatccgcc atgtgcctgc gccgccgctg gtcgctggaa aacttccagc
     7141 tcttcctggt tgagcatccg ctggttcgtc acttaacccg ccgtctgatt tggggcgttt
     7201 atagcgccga aaaccagcta ctggcttgct ttcgcgtagc agaagataac agctacagca
     7261 ccgctgacga tgatcttttc accctgccgg aaggcgatat ctctatcggc actcctcacg
     7321 ttctggaaat atcaccaacg gatgctgccg cctttggtca gctttttgcc gactacgaac
     7381 tgctaccacc gttccgccag ctcgaccgta acagctacgc cctgacagaa gccgagcgca
     7441 atgccagtga actgacccgc tgggcaggca gaaaatgccc gagtggtcgg gtcatggggc
     7501 tggcgaataa aggctggata aagggcgaac cacaggatgg aggctggatc ggatggatga
     7561 tcaaaccttt gggtcgctgg tcgttaatca tggaaatcga cgaaggtttt gcggtgggta
     7621 tgtcaccagc cgaacttagc gctgagcagc tcttaagcaa gctgtggtta tgggaaggca
     7681 aagcggaaag ctatggctgg gggagtaatt caacacagga agcgcagttc tccgtactcg
     7741 atgccatcac cgccagcgag ctaattaacg atattgaagc cctgtttgaa taaggaaaga
     7801 gcatggacaa ggaattaccg tggctggcgg ataacgccca actggaactg aaatataaaa
     7861 aaggcaaaac accactcagt catcgcaaat ggccgggcga accggtgcct gttatcactg
     7921 aaagcatcat tcagacactg ggtgataaat tgctacaaaa agctgagaag aaaaaaaaca
     7981 ttgtctggcg ttatgaaaat ttttcactgg agtggcagtc cgccatcacg caggccatca
     8041 acttgatcgg cgaacacaaa ccctcaatcc cggcccggac aatggcggcg ctagtctgta
     8101 tcgcgcaaaa tgacagccaa cagttgctcg acgaaatcgt ccaacaagag ggactggaat
     8161 atgcgactga ggtggtgatt gcacgccagt ttattacacg atgttatgag agtgatcctc
     8221 tgctagtgac attgcagtat caggacgagg attatggcta tggttatcgc tcagaaacct
     8281 ataacgaatt cgatctccga ctgcgtaagc atctctctct ggcagaggaa agcagctggc
     8341 agcgttgcgc cgacaaactc attgccgcac tgccaggaat acccaaaatt cgccgtcctt
     8401 ttattgcact actcctcccg gaaaaacctg aaatagccaa tgaactcgcc agtcttgaaa
     8461 gctcccggtc aagtctccat tcaaaggagt ggttaaaggt cattgctact gataatacgg
     8521 cggtaaaaaa actcgaacga tactggggcc tggacgtatt tagcgatcgc gaagccagct
     8581 acatgtcaca agaaaaccgc ttcggctacg cggcctgcgc ctcccttttg cgcgaacaag
     8641 gactggcagc cgttccgcgc ctggcaatgt atgcccataa agaagattgc ggcagtctgc
     8701 tggtacaaat taaccatccg caagtcatcc gcaccttgct actggtggct gataaaaaca
     8761 aacccagcct gcaacgtgtg gctaaataca gtaaaaactt cccccacgcg acgctcgccg
     8821 cactggcaga actgctggcg ttaaaagaac caccagcccg cccaggttat ccaatcatcg
     8881 aagacaaaaa gctgcctgca cagcaaaaag cacgcgatga atactggcgt acgctgttac
     8941 aaacgctgat ggcatcgcag ccacaactgg cagcagaagt gatgccgtgg ttaagtactc
     9001 aagccagggc agtgctgaat agttatttat cggcaccgcc aaaaccggtt attgatagca
     9061 ccgataacag cagtctgcct gaaatgctcg tttcaccacc gtggcgtagt aagaaaaaaa
     9121 tgacagctcc acgtcttgat ttggcaccgc tcgaattaac tccgcaaatt tactggcaac
     9181 caggcgaaca agagaggctt gccgccactg agtctgcccg ttatttcagc acggaatctc
     9241 ttgcggaacg catggaacaa aaaagtggac gagttgtatt acaggaactg ggttttgggg
     9301 atgatgtatg gctgtttctg aattatatac tccccggaaa actggatgct gcacgcaatt
     9361 cactcattgt tcagtggcat tactaccagg ggcgggttga agagatcctg aatggctgga
     9421 actccccgca agcacaatta gcagaacagg cgctccgcag cggtcacata gaagcgttaa
     9481 ttaacatatg ggaaaatgac aacttctcac gttatcgtcc ggaaaagagt gtctggaacc
     9541 tgtatttatt ggcacagttg ccgcgtgaga tggctttgac cttctggctg cgtatcattg
     9601 agaaaaagca tctgttcgcg ggtgaggact attttctcag tatcctcgga ttggatgcgc
     9661 taccaggtct gctgttggct ttttcacatc gtccaaaaga aacatttccg ttaattttaa
     9721 attttggcgc aacagaactg gcgctgcctg ttgcccgcgt ctggcgacgt ttcgcggcgc
     9781 agcgtgatct ggctcgccag tggattttac aatggccgga acatacggct agtgcactta
     9841 tccctcttgt ctttaccaaa cccagcgata atagcgaagc cgcattactt gccctgcgtt
     9901 tactgtacga acagggacat ggcgaattgc tacaaaccgt ggcaaaccgc tggcagcgta
     9961 cagatgtatg gtctgccctg gagcagttgc ttaaacaggg tccaatggac atttacccgg
    10021 cacgcattcc aaaagcccct gatttctggc atccggcaat gtggtccagg cctcgactta
    10081 tcactaataa tcagcctgtt accggtgacg ctctggaaat tatcggcgaa atgctgcgct
    10141 ttacccaggg gggacgtttt tatagcgggc tggaacaact gaaaacgttc tgccagccac
    10201 aaacgctggc agcttttgcc tgggatctct tcactgcgtg gcaacaagct ggtgcccccg
    10261 caaaagacaa ctgggcattt ctggcgttaa gtctctttgg tgacgaaagc acggcacggg
    10321 atctgacgac acagatcctc gcctggccac aagaaggcaa atctgcccgt gctgtcagcg
    10381 gcctgaacat ccttaccctg atgaataatg atatggcgct gatacagctg catcatatat
    10441 cgcaacgggc taaatcccgc cccttacgtg ataacgcggc ggaatttctt caggtggtcg
    10501 cagaaaatcg cgggctaagc caggaagagc tagcggacag attagtccca accctgggcc
    10561 ttgatgatcc gcaggcgttg agttttgatt ttggtccccg gcagtttacc gttcgcttcg
    10621 atgaaaacct caacccggtt atctttgatc agcaaaacgt tcgccagaaa agcgttcccc
    10681 ggttgcgcgc cgatgacgat caactgaaag cgcccgaggc actggcccga ctaaaagggc
    10741 taaaaaaaga tgctactcag gtgagcaaaa acctgctccc gcgtcttgaa gctgccctac
    10801 gtaccacccg acgctggtcg ctggcagatt ttcatactct gtttgttaat catcccttta
    10861 cccgcctggt tacccagcga ttaatatggg gcgtgtatcc ggcaaatgaa ccgcgtcgtt
    10921 tactcaacgc ctttcgtgtg gccgcagagg gggagttctg caatgcgcag gatgagccaa
    10981 ttgacctgcc tgcggatgct ctgattggca ttgcccaccc gttagaaatg acagcagaaa
    11041 tgcgcagtga atttgcccag ctttttgccg attacgaaat tataccgcca cttcgccagt
    11101 tgacacggcg cacggtgctg ctcacgcctg acgagtcagc cagtaatagt ttgaatcgct
    11161 gggaaggtaa atctgctacc gttgggcaac ttatgggaat gcgatacaaa ggctgggagt
    11221 caggctatga ggacgcattt gtctatgacc tgggtgagta ccggctggtc cttaagtttt
    11281 cacccggttt taaccactac aatgttgata gcaaagcgct aatgagcttc cgttctcttc
    11341 gagtgtacag tgacaataaa tccgtcactt ttgccgaact tgatgtgttt gatttgagtg
    11401 aggcgttaag cgcacctgac gtcattttcc attaaaacac agcgggcagt gatgcaactg
    11461 cccgttatca acacgactta ccagcgggga aagcgatgat cgtgcaaaaa gagctggttg
    11521 ctatttacga ttatgaggtc cctgtacctg aagatccgtt ttccttcaga cttgagatcc
    11581 ataaatgctc tgaattattt acaggttccg tctatcgact ggagcgattc cggctacgtc
    11641 caacatttca tcaacgtgat cgagaagatg ctgacccgct aataaatgat gcgttgattt
    11701 atataagaga tgagtgtatt gatgagcgga aattacgagg tgaatcacct gaaactgtaa
    11761 tagcaatttt taatcgtgaa ctacagaata tattcaacca agaaatagaa taatatactc
    11821 taaataattc aaattggtcc gatccggcgc aacgtcccaa cggtctggat tataaatctc
    11881 attatcttaa ttgcaacggg gtccagccgt ggttataatt cgttcgttgg aacacgatgt
    11941 tgcacaggct gtggtgttag gcctgaaaat agtaaataaa agaacctgcc tcaccagcag
    12001 gtttttttat ttactgcgat ctgctttcca gatatttttt gctcaaacaa ctaatgcgcc
    12061 aaacatttat tgcgcgtaaa atatcgttta tttcattaat acatttcagg gatgaatata
    12121 tgtcaccgca gaataatcat ctgcagcgtc cgcctgctgc tgtgttatac gccgatgaac
    12181 tggcaaaatt aaaacaaaat gataacgctc cttgcccgcc cggttggcag ttaagtttgc
    12241 ctgcggcccg tgcttttatc cttggcgata gcgcgcaaaa tattagccgt aaagtggtga
    12301 ttagtccctc cgctgtcgaa cgcatgttag tcactcttgc caccgggcgc ggtttgatgt
    12361 tggtggggga acctggcacc gcaaaatctc tcctgtctga attactggca accgctatta
    12421 gcggcgatgc cgggttaacc atccagggcg gcgcatctac taccgaagat caaatcaagt
    12481 acggctggaa ctacgccctg cttatcaacc atggtccgtc aacggaagca ctggtccctg
    12541 ccccgcttta ccagggaatg cgcgacggca aaatcgtccg ttttgaagag ataacgcgta
    12601 caccgctgga agtacaagac tgtctgttag gtatgctttc tgacagggtg atgacggtgc
    12661 cggaactcac cggtgaagcc agtcagctct atgcgcggga aggctttaat attattgcca
    12721 ctgccaatac ccgcgatcgc ggcgtcaatg agatgagtgc cgcgctaaag cgtcgcttcg
    12781 attttgaaac cgtctttccg attatggatt tcgcccagga gctggaactg gtcgccagtg
    12841 cttcggcgcg tttgctggcc catagcggta ttccgcataa agtgcctgac gcagtactgg
    12901 agttactggt acgcaccttc cgcgatctgc gtgccaacgg cgaaaagaaa acgtcaatgg
    12961 atacactaac agcgattatg tccaccgctg aagccgtgaa cgtggcccac gccgtaggcg
    13021 tccgcgcctg gtttttagcg aatcgcgcag gtgagccagc cgacctggtg gactgcattg
    13081 ccggaaccat cgtcaaagat aacgaagaag atcgcgctcg tctgcgccgt tacttcgaac
    13141 aacgagtcgc cacacataaa gaagctcact ggcaggctta ttatcaagcc cgccaccgcc
    13201 tgccgtgagg aaagatgcat gagcgagccg ttaattgtcg gcatccggca tcatagtccg
    13261 gcctgcgccc ggctggtgaa atcgttaatc gaaagccagc ggccacgata cgtattgatt
    13321 gaaggcccgg ctgattttaa tgaccgggta gacgaactgt ttttagccca ccagcttccg
    13381 gtagctattt acagttattg ccagtatcag gacggtgcag cccccgggcg tggtgcctgg
    13441 acgccatttg ctgaattttc gccggagtgg caggcgctac aagccgcacg tcgtattcag
    13501 gcacaaactt acttcatcga tttgccttgc tgggcgcaga gtgaagaagt ggacgattcg
    13561 cctgatacgc aagaggaaag ccaggccttg ctgctgcgtg ccacccgcat ggataacagc
    13621 gacaccctgt gggatcactt gttcgaagat gaaagccagc aaactgcatt accctctgca
    13681 ctggcgcact attttgccca actgcggggc gactcccccg gagatgctct caatcgtctg
    13741 cgcgaagcct ttatggcccg ctggattgga tgggcgatgc agcaaaataa tggcgacgtg
    13801 ttagttgtct gcggtggctg gcacgctccg gtactggcaa aaatgtggcg cgaatgcccg
    13861 caggaaatta acacgccaga attgccgtcg ctggcagatg ccgttacagg ttgttatctc
    13921 accccctaca gtgaaaagcg ccttgatgtg ctggcaggat acctttccgg aatgcctgcc
    13981 ccggtctggc aaaactggtg ctggcagtgg ggcttacagc aggcgggaga acaactgcta
    14041 aaaacggttc tcacccgttt gcgccagcac aagttgcctg cttcgaccgc ggatatggct
    14101 gccgctcatc tgcatgcgat ggcactggca cagttgcgcg gtcatacact accgttacgc
    14161 actgactggc tggatgccat agcaggatcg ctgattaaag aagccctgaa tgcgccgttg
    14221 ccgtggagct atcgcggcgt tattcatccc gacaccgatc cgattctgct aacgttgata
    14281 gacacattag cgggtgacgg attcggtaaa cttgcccctt ctacgccaca acctcctctg
    14341 ccaaaagatg tcacctgcga actggaacgt accgcaatct cccttccggc ggagcttacc
    14401 ttaaatcgct ttaaccccaa tggactagcg caaagtcagg tgttacatcg gctggcaata
    14461 ctggagatcc cagggattgt gcgccagcag ggaagcacac tgacacttgc aggcaacggt
    14521 gaagaacgct ggaaattaac ccgcccgctt agccagcatg cggcattgat tgaggccgcc
    14581 tgctttggtg caacactcca ggaagccgca cgccataaat tagaagccga tatgctggac
    14641 gcgggtggaa tcggcagtat caccacatgt cttagccagg cggcgttagc gggtctggcg
    14701 tccttcagtc aacaattact ggagcaactc acattattaa tcgcccagga aaatcaattt
    14761 gccgaaatgg gccaggcgct ggaagtgcta tatgccttgt ggcggctgga tgaaattagc
    14821 ggtatgcaag gcgcgcagat attacaaacg acgttatgcg cggctatcga tcgcacgctg
    14881 tggctatgtg aatctaacgg cagaccagat gaaaaggagt ttcacgctca cctgcatagc
    14941 tggcaagcgt tgtgtcatat cctgcgcgat ctacatagcg gtgtgcaact ccccggcatt
    15001 tctctttctg cggcggtagc cttactggag cgacgcagtc aggcaattca tgccccggcg
    15061 ctggatcgcg gcgcggctca tggcgcactg atgcgtctgg aacatcctaa cgccagtgcc
    15121 gaagcggcgc tgacgatgct ggcgcagtta tccccggcac aatccggcga agcgctgcac
    15181 ggtttgctgg cgctggcccg ccatcaactg gcctgtcagc cgacatttat cgccggtttc
    15241 agcagtcatt taaatcaact aagtgatgcc gattttatca atgccctgcc cgatttacgc
    15301 gcggcgatgg cctggctacc accacgagaa cgcgggacgc tggcgcatca ggtgcttgag
    15361 cattatcaac tggcgcaact tcccgtttcg gcgctgcaaa tgccgttgca ttgtccacca
    15421 caggccattg cacatcatca acaactcgaa cagcaggcac tggcatcgct gcaaaactgg
    15481 ggagttttcc atgtctgaac tgaacgatct tctgaccacc cgtgagctac aacgctggcg
    15541 attaattctt ggcgaagcgg cagaaacgac gctttgtggg ctggatgaca acgcccggca
    15601 gatagaccac gcgctggagt ggctgtatgg gcgcgatcct gaacggctcc agcgtggtga
    15661 acgctctggt ggattaggtg gctcaaatct caccacccct gagtggatca acagtattca
    15721 cactctgttt ccgcaacagg tgattgagcg actggaaagc gatgccgtac tgcgctacgg
    15781 cattgaagat gtggtgacga atctcgacgt gctggaacgt atgcagcctt cggaaagcct
    15841 gctacgcgcc gttttgcaca ccaaacatct gatgaatccc gaagtactgg ctgccgcccg
    15901 ccagatagtg cgccaggttg ttgaagaaat tatggcgcga ctggcaatgg aagttcgtca
    15961 ggctttttct ggtgtccgcg atcgccgtcg ccgctcatta attccactgg cgcgaaactt
    16021 tgatttcaaa agtactctgc gcgccaacct gcaacactgg cacccgcaac acggcaagtt
    16081 gtatatcgaa tccccccgct ttaacagccg cattaaacgc caaagcgaac aatggcaact
    16141 ggtcttactg gttgatcaaa gcggatcgat ggtggactcg gtgatccact ctgcggtgat
    16201 ggcggcctgt ttgtggcagt tacccggcat tcgtacccat ctggtggcgt ttgacacaag
    16261 cgtggttgat ctcacggcag acgttgccga tccggtagag ttattaatga aagtacagtt
    16321 gggcggcggg accaatatcg ccagtgccgt ggagtatggt cggcaactta ttgaacaacc
    16381 agcaaaaagc gtcattatcc tcgtgagcga tttttacgaa gggggttcat catcattgct
    16441 gacgcatcag gtgaaaaagt gtgtccagag cggcatcaaa gtgctgggac tggcagcgct
    16501 cgatagcacc gcaacgcctt gctatgaccg cgatacggcc caggcgctgg ttaatgtcgg
    16561 cgcacaaata gccgccatga cgccgggcga gctggcatca tggcttgcgg agaatcttca
    16621 gtcatgaatt cactacgtcc ggaattatta gaactgacac cgcaggcact gacggcgtta
    16681 agcaatgccg gttttgttaa gcgcagtctt aaggaactgg aaaatggcaa cgtcccggaa
    16741 atcagccatg agaacggcgc tttaatcgcc accttcagtg acggtgtccg tacccagctg
    16801 gcgaacagcc aggcactgaa agaggctcag tgcagctgcg gggccagcgg catgtgccgt
    16861 catcgcgtga tgctggtgtt aagttatcaa cgactttgtg ctaccgtcca gcccacggaa
    16921 aaagaagaag agtgggatcc ggcaatctgg ctggaagaac tggctaccct gcccgatgcc
    16981 acccgcaaac gcgcgcaggc gctggtcgct aaaggcatca ccattgagtt gttctgtgcg
    17041 ccgggcgaaa ttccctctgc ccgcttaccg atgagcgatg tgcgttttta ttcccgtagc
    17101 agtattcgtt tcgcccgttg tgattgtatt gaaggcacac tttgcgaaca tgtcgtactg
    17161 gctgtgcagg ccttcgtcga ggccaaagcg cagcaagcag aatttaacca tttaatctgg
    17221 cagatgcgca gcgaacacgt cacatcatct gacgatccgt ttgccagcga agaaggccaa
    17281 acgtgtcgtc aatatgttca gcaattaagc caggcattat ggcttggcgg catcagccag
    17341 ccgcttattc actacgaggc agcattcaac cgcacattgc aggcggcaga ggcctgcaac
    17401 tggcgctggg tgagtgaatc actacgacag ctacgggcaa gcgttgatgc cttccacact
    17461 cgcgccagcc actatcatgc cggagaatgc ttacgtcagc ttgcggcatt aaacagtcga
    17521 ttaaattgcg cacaagagat ggcccggcgc gacagtgttg gtgaagttcc tcctgtgccg
    17581 tggcgcacgg tcgttggctc tggcattgcc ggagaagcaa agcttgatca tctgcggctg
    17641 gtgtctttag gtatgagttg ctggcaggat attgagcatt atggtttacg catctggttt
    17701 accgatcccg acaccggcag tattttgcac ctttcgcgca gttggccgcg aagtgaacag
    17761 gaaaactcac cggcagctac gcgtcggctg tttagttttc aggctggcgc actggcgggt
    17821 gggcaaattg tttcacaagc agcaaaacgc agtgccgatg gcgagctgct gttagctacc
    17881 cgcaaccgct taagcagcgt tgtgccgctg tcgcctgatg cctggcaaat gttgagcgcg
    17941 ccgttacgcc agccgggcat tgtggctttg cgggaatatt tacgccagcg tccccccgcc
    18001 tgcatacggc ctcttaatca ggtcgataac ttatttattc tgccggtcgc tgagtgtatt
    18061 tcgctcggtt gggatagcag ccgccagacg ctggatgcgc aggtaatcag cggcgaaggt
    18121 gaagataatc tcctgacgtt atcccttccg gcatctgcct gttctccttt tgccgttgaa
    18181 cgcatggcgg cgcttttgca acaaacagac gaccccgtga gtctggtttc tggctttgtc
    18241 agttttgttg atgggcaatt gacactggaa ccacgggtga tgatgacaaa aacccgcgcc
    18301 tgggcgctgg acgcagaaac tgcgcctgtg gcaccgctac cttctgccag cgttttgcct
    18361 gtgccgtcta ccgctcatca gttgctgatg cgctgccagg cgttacttat tcaactgctc
    18421 cataacggct ggcgctatca ggaacagagc gctattagtc aggcagagtt gctggcgaat
    18481 gacctctccg cggtcggttt ttatcggctg gcacatgtgt tgggacaatt tcgtaataca
    18541 gaaagcgagg cacgggtaga agcaatgaat aacggtgttt tactttgcga acaattattc
    18601 cccatgcttc agcaacaagg atgaaatagt gctttttact aagagttcta ctccagttcc
    18661 ggactgctca cgtcacggta ttaggcatat tctatatagc ccctggtgag agtcaccagt
    18721 ttcttgatta aataaaatgg agttttacat gaaggctttc aataagctgt tttccctcgt
    18781 tgttgcatct gttctggttt tctctcttgc tggctgcggt gacaaagaag aatcgaagaa
    18841 attcagcgcc aatctgaacg gcactgaaat tgccattacc tatgtctaca aaggtgacaa
    18901 ggtgcttaag caatcttctg aaaccaaaat tcaatttgct tctattggtg caaccaccaa
    18961 agaagacgct gccaagacac ttgagccgtt aagcgccaaa tacaaaaaca tcgcgggtgt
    19021 tgaagaaaaa ttaacctata cagataccta cgcgcaggaa aacgtgacta tcgatatgga
    19081 aaaagtggat tttaaagccc tgcagggtat ttcaggaatc aacgtttctg ctgaagatgc
    19141 caaaaaaggt atcactatgg cgcaaatgga actggtgatg aaagccgctg gttttaaaga
    19201 agtgaaataa tctgtcggcg gccatgtttc gcatggccgc catacccgtc ttagcttttc
    19261 ttcacatgct ggcgcgctgc cagtccacgc agaaaataac gtaaaaattg atcgccgcat
    19321 tcgcggaagt ttttatgatc cggtgcgcgc atcatcgctg taatttccgg catcgaaacg
    19381 cggaactgct gttcggtgag gatagccaga atgtcatcgg ttttcagcga aaacgcgatg
    19441 cgtaattttt tcagcacgat gttgttatta atgcgacgtt ccggctccag tgccggagca
    19501 gactcatcct tgccgcgttt ttcataaatc aggccattga ggaatgacga caaaacaatg
    19561 tccggacaac gctgaaaacc ctcttcgtct tctttacgta gccagacggc gatctgttcc
    19621 gcggtggctt cgacattacc cagcgccaga atacgcacca ggtcattatt attggctttc
    19681 aaaatgtagc gcacgctgcg cagaatatcg ttacttagca tgaggccttc aggtgttgat
    19741 gaggcaaaaa gccattttag cagtctttta caggccaatc gcctctttta agcttttcag
    19801 ataacggcgg ctgaccggca cggttaagcc attacgcaaa atcaactcgg cctggccgtt
    19861 atcttccaga cgaatctcct gtaaatgcgc gaggttaacc agatactgac gatggcagcg
    19921 cagtagtggt gtacgacttt ccagggtacg taatgtcaat tcggtaaagc cctctttccc
    19981 ttcgtggctg gtaacgtaga caccgctcat ccgactgctg acaaatgcca catctttcat
    20041 ttgcagcaaa taaatccgac tatgccccgt acaagggata aatttcagcg cctgttgatt
    20101 ttccggtaac agcgaaacat cctgcttgct gcgttcctga cgcaaacgcg ccagcgtttt
    20161 ctccagtcgc gcttcatcaa ttggcttcag cagataatca aaggcatgtt cttcaaaggc
    20221 tttaatagcg tattcgtcaa acgcggtgag aaaaacaata tacggacgat gttccgggtc
    20281 gagcatcccc accatttcca gaccactgat gcgcggcatc tggatatcga gaaacagcac
    20341 atccgggcgc agtttatgca ccgcgccgat cccttccacg gcgtttgaac actctccaac
    20401 gatttcaata tcgctctgct cctgcaaaaa tacgcgcagg ttctcccgtg ctaacggttc
    20461 atcatcgaca attaagactt taatcatgcc tcgtccctcc atggtagtcg taacgttatt
    20521 cgggtgtaac tatcaggctc acaggcgacg cttattccat agtcatcgcc aaaccgttca
    20581 cgtaaacgct tatccaccag attcatcccc agcccactgg cattggttac cggttgatac
    20641 aaaccggcat tgtcttcgat ctccagcatc aaatgttgcc cctcacgtcg ggcgctgatt
    20701 gccactcgcc ctgtatccag cagttgtgat gtcccatgtt taatggcgtt ttccactatc
    20761 ggttgcaggg taaacgcggg caattgctgc tgggataatt cttgcggaat agcaatgttg
    20821 acctgcaacc gcgactggaa gcgcgccttt tcaatttgca gataagcgtt cacatgttca
    20881 atttcgtcgg cgagagtaac aaactccgaa ggccgcttta agtttttgcg gaaaaaagtg
    20941 gaaagatact gcaccagctg gctggcctgt tcgctgtcgc ggcggatcac cgctttaatg
    21001 gtgttaagcg cattaaacaa aaaatgagga ttcacctggg cgtgaagcag tttgatttct
    21061 gactgggtga gcatcgcttt ttgccgctca tattgcccgg caagaatctg cgccgaaagc
    21121 agttgcgcaa tcccctcgcc cagcgtgcgg tttattgaac tgaataaacg gtttttggct
    21181 tcatacaatt tgatggtgcc catcacgcgc tgattttcgc cacgcaacgg aattaccagc
    21241 gtcgacccca gtttgcattg cggatgcaaa gagcaacgat aaggtacttc gttgccatca
    21301 gcgtagacca cttcaccggt ttcaatcgct tttaaggtgt aagtcgaaga aatcggtttg
    21361 ccgggtaaat ggtggtcgtc accaattccg gtaaaggcca gcaatttctc tcgatcggta
    21421 atcgcgactg caccaatatc cagctcctga tacagcacct gagccacttt catgctgttc
    21481 acttcgttaa acccctgtcg caaaatgcct tccgtcgagg ctgccacttt cagcgcagtg
    21541 gcagaaaaag cagaagtgta tttttcaaac atcgcgcgtt tatcgagcaa tatacgcata
    21601 aacagcgccg cgccgacggt attggtgacc atcattggtg cagcaatatt actcaccaga
    21661 cgcaccgcat cttcataagg tcgggcgatc gcaaggatga tcagcatttg caccatttca
    21721 gcgacgaacg tgacggcacc ggcggtaatg gggttaaaga ctttatcagt gcgcccgcga
    21781 cggatcagga tgctgtgtac caggccgccg agcaatcctt caacgatggt tgagatcatg
    21841 caacttagcg cggtcatgcc ccccatcgaa tatcgatgta agccgccggt cagaccaacc
    21901 agcccaccga cgaccggacc gccgagtaag ccgcccatta ccgcgcctat cgcacgggta
    21961 ttggcaatag aatcgtcaat gtgcaaccca aaccaggtgc ccatgatgca gaagatggaa
    22021 aagacgatgt agcagagaaa tttatgcggc agacgaaccg tgacctgcat taacggtatg
    22081 aataatggcg ttttactcat taaccacgca atgactaaaa aaacgcacat ctgctgaagc
    22141 agcagcaaca ccagattaaa atcgtacata cccgcaaacc acacttccct ttaaaacgcg
    22201 taacatacat tgcctgcgtt taactttctt tgaactcttg cagaaaaatg agaattcgtg
    22261 agtacgatca ctcaaaatcg cctggcaaaa ataaaatcac cctatagatg cacaaaaaac
    22321 gggcaaaact acctggttcg caaaactgcg tctaaagtta aaccgggacc tcgcgagcaa
    22381 gggtgagacg atggcgcttt acacaattgg tgaagtggcg ttgctttgtg atattaaccc
    22441 tgtcacgtta cgcgcgtggc agaggcgtta cgggttgctg aaaccgcaac ggacagacgg
    22501 cggtcatcga ctgttcaacg atgccgatat tgaccgtatc cgcgagatca aacgctggat
    22561 cgacaacggc gtgcaggtca gcaaagttaa aatgctgctc agtaatgaaa atgttgatgt
    22621 gcagaacggc tggcgcgatc agcaagaaac attactgacc tacctgcaaa gcggcaatct
    22681 gcatagcctg cgaacgtgga tcaaagagcg cggtcaggat taccccgccc agacactcac
    22741 cacacatctg tttattcctc tgcgccgacg acttcaatgc caacaaccga ctctccaggc
    22801 gctgctggcg atcctcgacg gcgtactgat caactacatc gccatttgtc tggcttcggc
    22861 acgtaaaaaa cagggtaaag atgcgctggt ggttggctgg aatattcagg ataccacccg
    22921 tctgtggctg gagggctgga ttgccagtca acaaggatgg cgcattgatg tcctcgccca
    22981 ctcgctcaat caactacgcc ctgaactatt cgaaggccgt acattgctgg tgtggtgcgg
    23041 tgaaaatcga acctccgccc aacagcagca actcaccagt tggcaagaac aaggccatga
    23101 tattttccca ctcggcattt aatgattcgt taacaaatgc gctttactgt acaatccttt
    23161 cgttaacata aggagtgcaa tatgcgcata gctaaaattg gggtcatcgc cctgttcctg
    23221 tttatggcgt taggcggaat tggtggcgtc atgctcgcag gttatacctt tattttgcgt
    23281 gctggctaag cgcctgcacc agcctttcaa acaggcggtc tgcgatgatc gccgccagtg
    23341 ccaccagtaa cgccccctgg atcacatacg cggtattaaa tccgctaagc ccgatgatga
    23401 tgggcgtacc cagcgtgctg gcccctaccg ttgaggcgat cgtcgccgta ccaatgttga
    23461 taatcaccga agttcgcacg cccgccagaa tcaccggagc cgccagcggt agctcgacct
    23521 tacgcagtcg ctgaccacga ctcattccca tacctttcgc aacttctgtc acgctggcat
    23581 caatcgctcc cagcccggca agtgtcgcct gcaggacggg cagcacaccg taaaggatca
    23641 aggcgataat cgctggttgc agaccaaagc cgatcaccgg aacggcaatc gccagcactg
    23701 cgacaggcgg aaaagtctgt ccaacggcgg caatagtttc caccagtggg cgaaattccg
    23761 cgccccacgg gcgagtgaca gcaattccgg caccagtgcc aatgatcacc gcaaacaaac
    23821 tcgaaattcc caccagccag aaatgagcca gtgccagagc tgcaaaactt tcttgctgat
    23881 aaacgggtcg tggcagttgt gggaacaagg cagcaaacag cggctggctg taaggcagcc
    23941 agaaaatcag tgccacaaac aaagcaatga gccagaacag cggatcgcgc aacatcttca
    24001 tacgcttacg cctcctccag cagatcctga aaatgcagcg tgccgcaagg ctggccctgc
    24061 gtgttcacca ccggcagcac ctcgcatccc cgcgcgacaa acagggagag cgcatcgcgt
    24121 agcgtcatct cttctgccag tgcctcacct tctgcccgtt cttcgcgacg cacgtaatcc
    24181 gccacactac gtaacgaaag caggcgcaca cccagttcac tacgtccaaa aaactggcgg
    24241 acaaaatcat tcgccggacg agtcagcatc gtcagcggat tcccctgctg caccacttca
    24301 ccgtgatcca tcaataccag atgttctgcc agccttagcg cctcatcaat atcatgagtg
    24361 accagcacaa tagtgcgccc cagcaaacgg tgaatgcgcg tcatctcttg ttgcaacgcg
    24421 ccgcgcgtta ccgggtccag tgcgccaaaa ggttcatcca tcagtaagac ttgcggatcg
    24481 gcagccagtg cgcgcgccac tcccacacgt tgctgctgac caccggaaag ctgatgcgga
    24541 taacgctcac gcaaatttga ctccagcccc agtagcgcca ttaattcgtc gatacgatcg
    24601 tcaatccgcg cccgcgacca tttttgcaat tgcggcacgg tagcgatgtt ttgcgccacg
    24661 ctccagtggg ggaacaggcc aatagattga atggcatagc ccatccggcg gcgcaactcc
    24721 agtactggca gcgagcgaat ttcttctccg gcaaagcgga tcacgccgct gtcatgttcc
    24781 accaggcggt taatcatttt cagggtggtg gatttgccgg agccagatgt gccaatcagc
    24841 accgaaaaac tcccttcctg aaaattgaga ttgagatcgt taacggcttt ttgtgcgcca
    24901 aacagtttgc tgacatggct aaattcaatc attacgtttc accttcagca gtgcgataag
    24961 caaatcgaac agcgcgtcgg taagcaccgc cagaacaatt accgggatca cccccagcaa
    25021 cactaaatca atggcgctgc ttagcagccc ctggaaaacc agcgcaccaa aaccgcctgc
    25081 gccgattaac gccgcaatca ccgccatacc tacagtttgc accatcacca cccgcaggct
    25141 gcgcagaaat accggtaacg ccagcggtaa ctgaacatgc aggaaacgct gcgccccgct
    25201 catccccatc gctctggcgc tctccagcac atcgcgcggg atctggttca aaccgactac
    25261 cacgccgcgc accagcggca gcaaggcata gagcaccagc gcaatcagtg cgggtgtcat
    25321 tccggttcct gctatgccga gcttccccag ccacggaaag gccgtaacca gcgcggcaag
    25381 cggcgcaatc aacaggccaa agagcgccac cgaaggcacg gtctgaatga cattgagcag
    25441 agaaaaaatt gccccctgcc gcgcagtgga aaagtagcac cagatgccca acggcacacc
    25501 aatcactaac gcaggcagca ccgcaccaaa cagcaacgtc agatgttgtg ccagcgcgtc
    25561 gtcaaacaca tcctgacggt tggcgtattc tttcattagt gagagatcgt taagcgtgcc
    25621 ggagtacagc aaccacagcg gaataatggc aatctgcata tgcaacaacc agcgccacag
    25681 cggatgcgtg gagattcggc ggatggcatc gctacaggcc agcaatgcca gcgccgcagc
    25741 cagccagaaa ccactgccga ggctggtacg cgccagcgca ctgccatttt gcgccagttg
    25801 ggtcgccgcc tttccagctc cccacaccag caatacgaag acgaattgca ccagaatgag
    25861 tgcaaaaatg ctgccttttt taccgggaat aaaacaggct gtcagccagg cgcaaccaac
    25921 gcccaccagc atccagatcg tttgcggcca cagctgccag agatgtcgcc cttcacccga
    25981 gactaaacga ttaggcgcgt aactgataaa cggcagcgcc gctgcgatta ccgtcagcaa
    26041 cagcagcagc gccagaacag gattaatacg tagataagtc acgggaaatt acttcgccca
    26101 ccctttttgt ttcaggtagt cggcagccac ttttttggca tccagtcctt ccactgcaat
    26161 gctggcattc agttgctgca atgtttttgc atcgaggctg gcgaagactg gctgtagcca
    26221 ctgtgccatt tgcggatact ccttcaacac cgattcacgc accactggtg caggcgcata
    26281 gataggttgc acgccttgcg gatcgcttaa ggtttgcagc cccagcgccg ctaccgggcc
    26341 gtcagtgccg taagccattg cagcattaac gccagaggtt tgctgggcag cggctttgat
    26401 cgtcaccgcc gtgtcgccac cagccagtga cagcaactga tcctgaccga gcttaaagcc
    26461 ataggctttt tcaaacgcgg gtaacgcatc ggcgcgttcg ataaactctg ccgaggctgc
    26521 cagtttgaag gtgccgccct cttgcagata acgactcagg tcggcaagcg aagtgagttt
    26581 atttttctct gccacatcct gacgcacggc gatagtccag gtgttatttg caggcgcggg
    26641 cgtcagccag attaacttgt tgtgctccga atcgagcttt ttgactttct cgtaaccttg
    26701 ctgcgcgttt ttccacgctg catcgttttc atctttaaag aagaaagcgc cattgccggt
    26761 atattccgga tagatatcca gttcaccgga agtaatcgcc ccgcgcacca caggagtcgt
    26821 tccgagttgc actttattca ccgtcggtac gccgtggctt tccagcacct gcaaaatgat
    26881 attgccgagt agcgcacctt cggtatcgat ttttgaaccg actttaacgg gggaagccgc
    26941 ctgtagcggc aggctcacgg ctgccaacat aaccagtgaa cctgcccaga gctttaagag
    27001 tggcatgatg ctttcctcat tcttttactg ttgttttcag cgaattaaga gaaaagcata
    27061 gttgataatg gcggggttag cttgagcgga aggattcagt tgcagaatca gataaatact
    27121 taaggggcat attcggtccg gcgtttcctg ccggatgcgg cgcgagcgcc ttatccggcc
    27181 tacaaagggc gcaaacgtcg cgcccttact aaagcattac agcaactcaa actcgccttt
    27241 cttaacgcgt gcggaatcag tgccgataaa tacattgaac ttgccaggct cggcgtcata
    27301 tttcatctgt tgattccaga acttcagcgc ctcaatatcg atcgggaagc tgacagtctg
    27361 agtttcgccc ggtttcaggg tgattttctc aaagcctttc agctgtttca cagggcgact
    27421 catggaagcc gtcacatcct gcaagtacat ctgcactacc gtggccccct cgcgcttacc
    27481 ggtgttcgtc acctgcacgc tggcagtcac tttgccgtca cgcttcatgg tcggcgcaga
    27541 aagtttcaca tcagagacgg tgaaagtggt gtagctcagc ccatagccga acggatacaa
    27601 cgccccgtta gcttcatcaa aataacgcga agtgtatttg ttcggcttgt cggcattata
    27661 cgggcgaccg gtattcagat ggctgtagta caccgggatc tgcccgacag aacgcgggaa
    27721 ggacattggc agcttgccgg acgggttgta atcgccaaac aatacatcgg caattgcatt
    27781 accgccttca gtccccgcaa accaggtttc cagaatcgca tcagcctgct gatcttcttt
    27841 caccagcgcc agcggacgcc cgttcatcag caccagcacc agcggtttac cggtggcttt
    27901 cagcgccgca atcaagtcac gttggctttg cggaatagtg atatcggtcc ggctggaggc
    27961 ttcgtgcgcc atcccctgtg cttcaccgac tacagccacc accacatcag attgtttcgc
    28021 cgtctgcacc gcttcatcaa tcatctcttg cggcgaacgc ggatcgactt tgaccgcttc
    28081 ttcatactga ttcaggaaat cgataatgcc tttgtcactg gtaacgttcg cccctttggc
    28141 atacagcact ttaccgtttt caccgacgga atttttaatc ccggtcagta cggtcacgga
    28201 ttgatcggca acaccggctg cggaccagct gcccatcacg tcacgtttac tgtccgccag
    28261 tggcccaacc accgcaatgg tggccgattt tttcagcggt aacgtttcga gacggttttt
    28321 cagcaacacc aggctttcgc gcgccacttc acgcgcttct ttacggtgca ggcggctttc
    28381 ggcattggta tccaccgggt cagactcttt cggccccaaa tggctgtatg ggtcgttaaa
    28441 caatcccata tcatatttaa cgttcagtac atggcgggca gcatcgtcca gctcttccat
    28501 cgtcactttg ccggatttga tcaacccagg cagatacttc gagtaatact cgtcgctcat
    28561 actcatgttg attccggatt tcagcgccac gcgcaccgca tcttccgggt ctgccgccgt
    28621 gccatgttta atcagctctt tgattgcacc gtgatcggaa acggtgatgc ctttaaagcc
    28681 ccactggtcg cgcagaacat ctttcagcag ccaggaatcg gaggtggctg gcgtgccgtt
    28741 cagcgagttc agcgccacca tcaccgcgcc gctgcctgcg tccagccccg ctttgtacgg
    28801 cggcatataa tcattaaaca ggcgctgcgg actcatatcg acggtgttgt actctttacc
    28861 gccttctacc gcgccgtatg cggcaaagtg tttgacgctg gtcatcaccg agtagcgatc
    28921 tgccgggctt ttaccctgca tcgcttccac catggtttta cccattgttg aggtgagata
    28981 cgtatcttcg ccaaaacctt cggaagcacg tccccagcgc ggatcgcgcg agacatcgac
    29041 catcggtgcc caggtcatat tcaggccatc atctgccgct tcataagcag agacacgtcc
    29101 gaccgttttc actgcatcga ggttaaaaga cgaggccaga ccgaggctaa tcgggaacac
    29161 cgtgcgctga ccgtgcagca cgtcgtaagc aaagaaaaga ggaattttca ggcggcttaa
    29221 ttccatcacc tgatcctgca tggcgcggat atcctgacgg gttacggtgt tgaaaatcgc
    29281 cccaacctga ccgtctttga tcatctcgcg gatcgcctct ttcgggttat ccgggccgac
    29341 gctgattaag cgcagctgac caattttctc atcaactgtc attttcttaa gcagttcggt
    29401 gacgaacgca tcccgcgctt cgggcgttaa tggatggttg ccgaataaat catccgccag
    29461 tgctggctgc agggccagac tcaccgcgat tcctactgaa catagccatt tcatatggat
    29521 ttttcttcct ctgttgcccg acgaggcagc gaaaatggtg caaaaaccgt agtttgccat
    29581 aagcatgatg gagagagaaa aagaatgctc agtttattgt ctgaattttc aaaatattca
    29641 ctcgctgaat tgtcatacaa ggcgctattc tagtttgtga tattttttcg ccaccacaag
    29701 gagtggaaaa tgtcttccat gacaacaact gataataaag cctttttgaa tgaacttgcc
    29761 cgtctggtcg gtcattcaca cctgctcacc gatcccgcaa aaacggcccg ctatcgcaag
    29821 ggcttccgtt ctggtcaggg cgacgcgctg gctgtcgttt tccctggctc actactggaa
    29881 ttgtggcggg tgctgaaagc ctgtgtcacc gccgacaaaa ttattctgat gcaggctgcc
    29941 aatacaggcc tgaccgaagg atcgacgcca aacggtaacg actatgatcg cgatatcgtg
    30001 atcatcagca ccctgcgtct cgacaagctg cacgttcttg gcaagggcga acaggtgctg
    30061 gcctatccgg gcaccacgct ctattcgctg gaaaaagccc tcaaaccgct gggacgcgaa
    30121 ccgcactcgg tgattggatc gtcgtgtata ggcgcatcgg ttatcggcgg tatttgtaac
    30181 aactccggcg gctcgctggt gcaacgtggc ccggcgtata ccgaaatgtc gttattcgcg
    30241 cgtataaatg aagacggcaa actgacgctg gtgaaccatc tggggattga tctgggcgaa
    30301 acgccggagc agatccttag caagctggat gatgatcgca tcaaagatga cgatgtgcgt
    30361 cacgatggtc gtcacgccca cgattatgac tatgtccacc gcgttcgtga tattgaagcc
    30421 gacacgcccg cacgttataa cgccgatccg gatcggttat ttgaatcttc tggttgcgcc
    30481 gggaagctgg ccgtctttgc ggtacgtcta gataccttcg aagcggaaaa aaatcagcag
    30541 gtgttttata tcggcaccaa ccagccggaa gtgctgaccg aaatccgccg tcatattctg
    30601 gctaacttcg aaaatctgcc ggttgccggg gaatatatgc accgggatat ctacgatatt
    30661 gcggaaaaat acggcaaaga caccttcctg atgattgata agttaggcac cgacaagatg
    30721 ccgttcttct ttaatctcaa gggacgcacc gatgcgatgc tggagaaagt gaaattcttc
    30781 cgtccgcatt ttactgaccg tgcgatgcaa aaattcggtc acctgttccc cagccattta
    30841 ccgccgcgca tgaaaaactg gcgcgataaa tacgagcatc atctgctgtt aaaaatggcg
    30901 ggcgatggcg tgggcgaagc caaatcgtgg ttggtggatt atttcaaaca ggccgaaggc
    30961 gatttctttg tctgtacgcc ggaggaaggc agcaaagcgt ttttacaccg tttcgccgct
    31021 gcgggcgcag caattcgtta tcaggcggtg cattccgatg aagtcgaaga cattctggcg
    31081 ttggatatcg ctctgcggcg taacgacacc gagtggtatg agcatttacc gccggagatc
    31141 gacagccagc tggtgcacaa gctctattac ggccatttta tgtgctatgt cttccatcag
    31201 gattacatag tgaaaaaagg cgtggatgtg catgcgttaa aagaacagat gctggaactg
    31261 ctacagcagc gcggcgcgca ataccctgcc gagcataacg tcggtcattt gtataaagca
    31321 ccggagacgt tgcagaagtt ctatcgcgag aacgatccga ccaacagcat gaatccgggg
    31381 atcggtaaaa ccagtaaacg gaaaaactgg caggaagtgg agtaaaaatt acggatggca
    31441 gagtatcgcc atccgaattc acttaatcgt tctgtgccgt ctgccccgcc gccgccattt
    31501 gggcggcttt ttgtttttta tagctcaacg ctgctgccgg cacaggcatc actttaccgg
    31561 tttcaatcca ggtacgcagg cggctggcat cggcaaaatg ggtatatttg ccaaacgcgt
    31621 ccatcactac cagcgccacc ggtttattat tgataaccgt acgcatcacc agacaatggc
    31681 ccgccgcatt ggtaaagccg gttttggtta actgaatatt ccagttatcg cgatacacca
    31741 gatgattagt attgcggaac ggcagcgtat acgtcgggtt agagaaggtt gccatatctt
    31801 cccgggtagt acttaactgc ccgatcaacg gatattgttt gctggcaatg agcagtttgg
    31861 ttaagtcacg ggcagttgaa acgttatgca ccgacaatcc ggtaggttca acaaagcgcg
    31921 tgttgttcat tccgagcgat ttcgctttcg cattcattgc cttaataaag gctttgtaac
    31981 caccgggata atggtgtgca aggcttgccg ccgcgcggtt ttctgaagac atcagcgcca
    32041 gcaacagcat atctttacgg ctgatttcgc tattcagtcg tacgcgcgaa tagacccctt
    32101 tcatctccgg cgtctggctg atatccactt ttagtttttc atccagcggc agtcgtgcat
    32161 ccagcacaac catcgcggtc attaatttgc tgatagacgc aatcggacgc accagatccg
    32221 ggtggttcga atagatcgct ttgttggtat tcagatcaac aatcatcgcg ctaccggagg
    32281 caatttccgg ttgtgaagcg gtggtagcgg ctgccgtttt cgcaacggcc tgcggtgcaa
    32341 aaggcacagc cagcatcagg gccaggctaa ataaagaaac tcgaaatttc ggcatgatga
    32401 gcattcagat agtggttcac gcgcacgggt tgcgcaccgc cggagtaagg atttactgag
    32461 gctagcgacg ccatcataac gagcaaaaag tgcgatcgtc aaaggagaat cgtgaggaaa
    32521 tgctgcattg ctgacattta cgccagcaat gcaacgtcag agaactttct agaacaaacg
    32581 ataaccgtag ccccatagta taacggttag ggcgagcagc acttccagta ccagcacgcc
    32641 aatcgccagc gtcgaactag agaagctaag gccttcctct ttgttgatat tcaggaagct
    32701 cggaatacca aggtaaagca ggtagccggt gtaaaacagc gccaccgtgc cgaccagcgc
    32761 acacaaccag accagtggat aaagcgccac cagaccgctt aaaaacagcg gagttgcaac
    32821 gtagcccgcg aagaccatgc agtgcgcaag tgacggacgc tgcggataat tacgcgccat
    32881 ccaccagatg acccgcccca tcaccgcgac cccagccagc ataacgccgt aaaacaagac
    32941 agccagcgcc agtccggtaa accaggataa cttcaggata gtgccatcgc caaaattcca
    33001 gccaatctgt gtagtgccaa tgaaggcgca aatcaccgga atcgccgcca tcagcaaaac
    33061 gtggtgggtg taatggtgag aaatcgtttc gttttcgcga ttaatcacct gcatttcacg
    33121 atcgggatgg gaaaacagtc cccagacatg gctcataccg cccccttgtt gtgagttcat
    33181 gaacctgaca gttcaagtat aagtcagctt gtgattattt tttgttcgcc catgtaattt
    33241 tcactgtctg atgaacgtcc tttttcagcg tgaatgattc acagggtgta tgcttactgg
    33301 caaccaaagg gagacagact ggcctatgga tctcaataca cttatctcac aatatggtta
    33361 tgccgcgctg gtgatcggta gcctggcgga aggtgaaacc gtgactttgc tgggaggcgt
    33421 tgcggcgcat caggggctat taaagttccc gctggtggta ctttctgtgg cgcttggcgg
    33481 catgattggc gaccaggtgc tctatctgtg cgggcggcgg tttggcggca agctgttacg
    33541 ccgtttctcg aaacatcagg ataaaattga gcgggcgcag aaacttatcc aacgccatcc
    33601 gtatctgttt gtcattggta cgcgctttat gtatggcttt cgggtgattg gcccgacgct
    33661 gattggtgcc agccagctgc cgccgaaaat ctttctgccg ctgaatattc tcggcgcatt
    33721 tgcctgggcg ttgattttta ccactattgg ttacgctggt ggtcaggtga ttgcgccgtg
    33781 gttgcacaat ctcgaccagc atttgaagca ctgggtctgg ttgattctgg tcgtggttct
    33841 ggtggtgggc gtgcgctggt ggctgaaacg gcgcgggaag aaaaagccgg atcatcaggc
    33901 gtaaaaccat tgccctgggt aaggcgttcg cgccgcatcc gacatccggt gcaccatgcc
    33961 tgatgcgacg cttgtgcgtc ttatcatgcc taccaaaatt ttacccattg aaataaccac
    34021 gcacctcact gcgctattct gggttgaact gcggattcgc caacataaag ccgccatcca
    34081 ctatcaacga ctgcccggtg gtgtaatttg cgccctctga gcacagccac gctaccaggc
    34141 tggcaatctc atgcgttgcg ccaaaacgcc gcagaggaat cgaaggctcc gcgtcgggct
    34201 tcacgtcgct gtcatccatg ccattcattg gcgtggcgat cgccccaggc gcgactgcgt
    34261 tcaccaaaat cttatgcctg accagctcca gcgccatcgt tttggttaac ccgccgagcg
    34321 catgtttagc ggctgtgtaa gcgctggcat ccggcagcgg cgtatgttca tgtaccgacg
    34381 taatgttgat gatgcgaccg ccctgccctt gtttcaccat ctggcgggcc gcaatttgtg
    34441 agcataaaaa tgcaccatcg acatcaacgg taaaaatctt gcgccactca tcgaaagcca
    34501 tatcaagaaa cggcgctttg gtcattgcgc ccgcattatt caccagcaca tcaatacgcc
    34561 ccagccgttg aatgagtttc tccagcgcct gcgcaccttc tggcagttta ccgagatcca
    34621 gctgcacgat ctccgcacgt acgccgtggc taactacctc acgcgcggta tcttttgccc
    34681 cttcttcatc tgagtgccag gtaatgccaa tatcaaaccc ctgctgcgcc agtaataacg
    34741 cgcactcttt gccgatcccc gaatcggagg cggtaataat cgcaacctgt gccatcgagt
    34801 tctccactta acgctgaata aacgttaagt atagaaggcg catatcatca gcgtttgtaa
    34861 cccccgccca acgcaccagt gagttgaatg gaagcatcca gccactgccc ttgcaataac
    34921 aggccattgg cccgctcacg cagcgcgggg attctggctt cgctgacgcg ggaaccagca
    34981 atgatgcccg cgttaaagcg cgcctgcgca agacctacca cacgcaaggc atcgcgctca
    35041 atttgcgcct gatgctggtt tttctccgca agtgtctgaa cctgactggc tgcccgcgcc
    35101 acgtcattca ccgcttcaac caccgctttg ttgtagctgg cgatagacaa gttgctttcg
    35161 gcttttgcga tatcgagatt ggcgttaaga cgaccactat cgaaaatggg tagcgtcagg
    35221 cctgccgtaa cgcccatttg ctgcgcggaa tgacggaaca gatcgcttaa gtgcaacgca
    35281 tcctgttgca ggaaggccat caggttgatg tcaggataaa atgccgcttt tgccgcatca
    35341 atggtgctta gcgatgactc aacgtaccag tgcgccgcct gcaaatctgc ccgccgggcc
    35401 agtaaggagt accccagttc atcaggaagc tggcttgcca ctttcggcaa cgcgaccggt
    35461 ttaagcttca atgactttgt ctggttattt gtaagtgcgc ttaaccgtgc ctcaataatt
    35521 ttcattttcc ccgcgacatc gttgagctgc tgccgggttt tgctggcatt aatatcggtt
    35581 tccacacctt caactgaaga agtaatcccg ttctgatata gctggcgatc ggtcgcgata
    35641 atggtgttct gctctttttc tatttgctgc aagaccgtgt ttaacgccgc ctgggtttgc
    35701 cactcccagt acaggcgggc tacgctgcca gccagcaatt ggcgggtttg ctcgcgttcc
    35761 gccgcccgtg ctttaaccgt acccaggcgg gcagtaacct ccgcccgatt ctttccccag
    35821 atatcgagat gccagcccgc cgttaagcca aaagttccgt tggtgtacca cggaccggtc
    35881 gtacctgcgg ccggatcgtt cagagcaaac ggccccatta agccttctgc cgacattttt
    35941 tgccgctcca tatccgcaga aaagtcgatc tgcggaccat cctgagtagc aattgccttc
    36001 gcctgggctt cagctagctg aatgcgctgt tcagccacct gcatatccgg tgcgttctgt
    36061 agtgcattgt taattaagga agtgagttga ttatcgtgat actccagcca ccattggctg
    36121 tctggccaac catttttcag cgccgtgggt aatgcggtgt caacttgtgc agcaggcgtt
    36181 tgctggctta acgcctggcg ggtttcatgc ataggtgcac acccggccag catcagtaac
    36241 agcggaaaac aggcgatggc tggataaaag gaatcacgat tcatggggga ataatcaggt
    36301 aagaaaaggt gcgcggagat taccgtgtgt tgcgatatat tttttagttt cgcgtggcaa
    36361 tacatcagta gcaataaaac gacatatcca gaaaaatata gactaagtaa atgatatctt
    36421 ccgatttatc ttaatcgttt atggataacg gcaaagggct tcgttttttc ctatacttat
    36481 tcagcactca caaataaagg aacgccaatg aaaattatac tctgggctgt attgattatt
    36541 ttcctgattg ggctactggt ggtgactggc gtatttaaga tgatatttta aaattaatta
    36601 atgtcatcag gtccgaaaat aacgagaata tttcagtctc tcatcctgtt gcgctcctgt
    36661 catgtgcatt gcttcatata atcactggcg caaggagcgc gcagggcggc caatcgccgc
    36721 cgccccctgc acccccgggc tctggcgaac aaaatcgccg ctgcgcggtg ccctcggctt
    36781 atcccttacg gctaccgggt cgggcgcgag gtaacatccc tgtaaaacgc gccctcagcc
    36841 cacatccatg tgggctgccc cggccttcag ggaacgcctc ggcaattttg acgccaccaa
    36901 acaaccgtgc ggcctattga taaagagcta acacattgtc aaaaaacatc actatggttt
    36961 tttagagttt ctcgatatca attgcctgaa tagcccttgc aatatcaggg gaattattca
    37021 acacccgaac atgctgaaat aattccgttg cttcatcgta ttctttacgc aaataactca
    37081 accactgttt aatccgcgca acgtgatata acccggtatc gccctgcttt tccagacggg
    37141 tatatttttg cagcaaagca accacctccg gccacggcat tcgcggttcg ttatatttta
    37201 ctacccggct caggttggga atattgagcg ccccgcgacc aatcatcact gcgtcgcagc
    37261 cgctgatcgc aatgcattgt tgcgcgctct gccagtccca gatttcgccg ttggcaatca
    37321 ccggaatatt cagccgctgg cgaatctcgc caatcgcctg ccagtcaata tgctccgcgc
    37381 ggtaaccctg ctcttttgtc cgtccatgca ccaccagctc cgtagcgcca gcctgttgaa
    37441 ccgcatcggc gatttcaaat ttcttctcac cgctgtccca gcccagacgc acttttacgc
    37501 tgacgggcaa atgcgctggt acagcttcac gcatcgcttt tgcaccctgg tagatgagtt
    37561 caggatcttt gagtaacgtc gcgccgccgc cgctaccgtt aaccgttttc gacgggcagc
    37621 cgcaattgag atccacgccc caggaaccta actccaccgc acgggcggcg ttctctgcca
    37681 gccattgtgg gaactgacct aacaactgca cgcgcaccag cgtaccagat ggtgtccggc
    37741 tggcgttttg tagctcaggg caaatgcgat gaaagacttt taccggcagc agttgatcca
    37801 ccacgcggac aaactcggtg atgcacagat cgtagtcgtt aacttcggtc agcaattcac
    37861 gcaccagaga gtcaagcaca ccctccatcg gagccagtaa cacacgcata tcttcacccg
    37921 caaaaaaatg aggcgccatg ttagcgccta tggtcaacgg attaaaggtc tgcaaagagg
    37981 gattgctgca aactgtaatc cctctctatg cttgatgtat gcctgagttc gttgcgcatt
    38041 gcgtaatgcc ggggagcgaa aaaataagcg ctgatttctt aatgtgatcg gtagcacgtt
    38101 ttacgaatta attgtatgat gaatccatct catctggggt gttgattatg agtaagacac
    38161 tgaacattat ctggcaatat ttacgcgctt tcgtcctgat ttatgcctgc ctgtatgcag
    38221 gcattttcat tgcttccctg ctaccggtaa ccattccggg cagcatcatc ggtatgctga
    38281 tcctgtttgt cctgctggcc ttgcaaattc ttccggcaaa atgggtcaat ccggggtgct
    38341 acgtactgat tcgctatatg gcgctattgt ttgtgccgat tggcgtaggc gtcatgcaat
    38401 attttgattt gctccgcgca cagtttggcc cggtagtggt ttcctgtgca gtcagtacgc
    38461 tggtggtttt tctggtggtg agctggagtt cacaactggt acacggtgaa cgtaaagtcg
    38521 taggtcagaa aggatcagaa gaatgatggc gaatatctgg tggtcattac cgctgacgtt
    38581 gattgtcttt tttgccgccc gcaaactggc ggcacggtat aagttccccc tgcttaaccc
    38641 gttgctggta gcaatggtgg tgatcattcc ttttttaatg ctcaccggca tctcttacga
    38701 cagctacttt aaaggtagcg aagtgctaaa cgatttgctg caaccggcgg ttgtcgcgtt
    38761 ggcttatcct ttatatgagc agctacacca gatccgcgcg cgctggaaat cgatcatcac
    38821 catctgtttt atcggcagcg tggttgcgat ggtgacgggg acttccgtgg cattgttgat
    38881 gggcgcttca ccggaaatcg ccgcgtcaat cctgccgaaa tcagtcacca cgcctattgc
    38941 aatggcggtt ggcggcagta ttggcggtat tccggcaatc agcgcagttt gcgtgatttt
    39001 cgtcggcatc ctcggcgcgg tatttggcca tacattgctt aatgcgatgc gtattcgtac
    39061 caaagctgcg cgcggtctgg caatggggac tgcctcgcac gccctcggta cggcgcgctg
    39121 cgccgagctg gattatcagg aaggtgcatt tagttcgcta gcgctggtgt tatgcgggat
    39181 aattacttcg ctgatcgcac cgttcctttt cccgattatt ctggcagtaa tgggctaaaa
    39241 tttgcgatgc gtcgcgcatt tttgatgtaa gtttcacgcg ttgcataatt aatgagattc
    39301 agatcacata taaagccata acgggttcgt aaactgttat cccattacat gattatgagg
    39361 caacgccatg catccacgtt ttcaaaccgc ttttgcccaa cttgcggata acttgcaatc
    39421 tgcactggaa cctattctgg cagacaagta cttccccgct ttgttgaccg gggagcaagt
    39481 ctcatcgctg aagagcgcaa cggggctgga cgaagacgcg ctggcattcg cactacttcc
    39541 gctggcggcg gcctgtgcgc gtacgccatt gtcgaatttt aatgttggcg caattgcgcg
    39601 cggtgtgagc ggaacctggt atttcggtgc caatatggaa tttattggtg cgacaatgca
    39661 gcaaaccgtt catgccgaac aaagcgcgat cagccacgcc tggttgagtg gtgaaaaagc
    39721 gcttgcagcc atcaccgtta actacacgcc ttgtggtcac tgtcgtcagt ttatgaatga
    39781 actgaacagc ggtctggatc tgcgtattca tctgccgggc cgcgaggcac acgcgctgcg
    39841 tgactatctg ccggatgcct ttgggccgaa agatctggag attaaaacgc tgctgatgga
    39901 cgagcaggat cacggctatg cgctgactgg tgatgcgctt tctcaggcgg cgattgcagc
    39961 ggcaaaccgt tcgcacatgc cttacagtaa gtcgccaagc ggtgtcacgc tggaatgtaa
    40021 agacggtcgt attttcagcg gcagctacgc tgaaaacgcc gcattcaacc cgactctgcc
    40081 accgttgcag ggagcgttaa ttctattgaa tctcaagggt tatgattacc cggatatcca
    40141 gcgcgcggtt ctggcagaaa aagccgatgc gccgttgatt cagtgggatg ccacctccgc
    40201 aacactgaaa gctctcggct gtcacagtat cgaccgagtg cttctcgctt aagcctggtg
    40261 ccggatgcgg cgtgaacgcc ttgtccggct tgccagccct ctcctggtgt cgaaattccc
    40321 ggcaaacagt ttgccgtttc ttgcgcaaaa ccagcgggta aagtagcctg atggaaattt
    40381 tccttagatc gagtctcctg catgttaaag cgcgtgttcc tcagcctgtt agtcctgatc
    40441 ggcttgctgc tgttgactgt gctcggcctc gatcgctgga tgagctggaa aaccgcgcct
    40501 tatatctacg acgaattgca ggatctcccc taccgccagg tcggtgtggt gctcggaaca
    40561 gcaaaatatt atcgtactgg cgtaattaat cagtattatc gctaccgcat tcaaggagcg
    40621 attaatgcct ataacagcgg taaggtaaat tatctattac tgagcggcga taacgcattg
    40681 caaagttata atgagccgat gaccatgcgc aaagatttaa tcgctgctgg tgtcgaccca
    40741 tcagatattg ttctcgatta cgcaggcttt cgtacgctgg attccatcgt gcgtacacgc
    40801 aaagttttcg atactaatga tttcattatt atcacccaac gtttccactg tgagcgggca
    40861 ttatttattg cgctgcatat ggggattcag gctcagtgtt atgccgtacc gtcaccgaaa
    40921 gatatgctgt cagtacgtat tcgcgaattt gccgcccgtt tcggtgcgct ggctgacctt
    40981 tatattttta aacgtgaacc gcgtttttta gggccgctgg tccctattcc ggctatgcac
    41041 caggtgccgg aagatgcgca ggggtatccc gccgtcacac ccgaacagtt acttgaatta
    41101 caaaagaaac aaggaaagta gttatggatg tacagcagtt ttttgtcgtt gccgtttttt
    41161 tccttattcc gatattttgt ttccgcgaag catggaaagg ctggcgcgca ggcgcgattg
    41221 ataaacgggt taaaaatgca ccggaaccgg tgtatgtctg gcgagcaaaa aatcccggac
    41281 tctttttcgc ttatatggtg gcatatatcg gcttcggaat ttt
//
LOCUS       DAJOWB010000028        40710 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJOWB010000028 DAJOWB010000000
VERSION     DAJOWB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.028"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(22..387)
                     /locus_tag="OHO85_001688"
     CDS             complement(22..387)
                     /locus_tag="OHO85_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCQ2671071.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(680..1666)
                     /gene="yqjG"
                     /locus_tag="OHO85_001689"
     CDS             complement(680..1666)
                     /gene="yqjG"
                     /locus_tag="OHO85_001689"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCQ2671072.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQNEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DTLGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQEAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(1736..2128)
                     /locus_tag="OHO85_001690"
     CDS             complement(1736..2128)
                     /locus_tag="OHO85_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCQ2671073.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(2314..2613)
                     /locus_tag="OHO85_001691"
     CDS             complement(2314..2613)
                     /locus_tag="OHO85_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCQ2671074.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(2603..3007)
                     /locus_tag="OHO85_001692"
     CDS             complement(2603..3007)
                     /locus_tag="OHO85_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCQ2671075.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(3010..3315)
                     /locus_tag="OHO85_001693"
     CDS             complement(3010..3315)
                     /locus_tag="OHO85_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCQ2671076.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(3353..3721)
                     /locus_tag="OHO85_001694"
     CDS             complement(3353..3721)
                     /locus_tag="OHO85_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCQ2671077.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(3868..4251)
                     /gene="mzrA"
                     /locus_tag="OHO85_001695"
     CDS             complement(3868..4251)
                     /gene="mzrA"
                     /locus_tag="OHO85_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCQ2671078.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(4255..4917)
                     /locus_tag="OHO85_001696"
     CDS             complement(4255..4917)
                     /locus_tag="OHO85_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCQ2671079.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(5262..6038)
                     /gene="exuR"
                     /locus_tag="OHO85_001697"
     CDS             complement(5262..6038)
                     /gene="exuR"
                     /locus_tag="OHO85_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCQ2671080.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            6334..7050
                     /locus_tag="OHO85_001698"
     CDS             6334..7050
                     /locus_tag="OHO85_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCQ2671081.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            7080..7580
                     /locus_tag="OHO85_001699"
     CDS             7080..7580
                     /locus_tag="OHO85_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2671082.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            7672..10356
                     /locus_tag="OHO85_001700"
     CDS             7672..10356
                     /locus_tag="OHO85_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCQ2671083.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            10353..11441
                     /locus_tag="OHO85_001701"
     CDS             10353..11441
                     /locus_tag="OHO85_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCQ2671084.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(11529..12827)
                     /gene="exuT"
                     /locus_tag="OHO85_001702"
     CDS             complement(11529..12827)
                     /gene="exuT"
                     /locus_tag="OHO85_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCQ2671085.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            13310..14722
                     /gene="uxaC"
                     /locus_tag="OHO85_001703"
     CDS             13310..14722
                     /gene="uxaC"
                     /locus_tag="OHO85_001703"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCQ2671086.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            14737..16224
                     /gene="uxaA"
                     /locus_tag="OHO85_001704"
     CDS             14737..16224
                     /gene="uxaA"
                     /locus_tag="OHO85_001704"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCQ2671087.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            16307..16858
                     /locus_tag="OHO85_001705"
     CDS             16307..16858
                     /locus_tag="OHO85_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCQ2671088.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEVSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(16863..18107)
                     /gene="sstT"
                     /locus_tag="OHO85_001706"
     CDS             complement(16863..18107)
                     /gene="sstT"
                     /locus_tag="OHO85_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCQ2671089.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(18506..19471)
                     /gene="alx"
                     /locus_tag="OHO85_001707"
     CDS             complement(18506..19471)
                     /gene="alx"
                     /locus_tag="OHO85_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCQ2671090.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(19754..20740)
                     /locus_tag="OHO85_001708"
     CDS             complement(19754..20740)
                     /locus_tag="OHO85_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCQ2671091.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFRADSVKL"
     gene            complement(20819..21502)
                     /locus_tag="OHO85_001709"
     CDS             complement(20819..21502)
                     /locus_tag="OHO85_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCQ2671092.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(21579..22082)
                     /locus_tag="OHO85_001710"
     CDS             complement(21579..22082)
                     /locus_tag="OHO85_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCQ2671093.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            22167..23303
                     /gene="rlmG"
                     /locus_tag="OHO85_001711"
     CDS             22167..23303
                     /gene="rlmG"
                     /locus_tag="OHO85_001711"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCQ2671094.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            23494..23577
                     /locus_tag="OHO85_001712"
                     /pseudo
     CDS             23494..23577
                     /locus_tag="OHO85_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            23574..23990
                     /gene="higA"
                     /locus_tag="OHO85_001713"
     CDS             23574..23990
                     /gene="higA"
                     /locus_tag="OHO85_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCQ2671095.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(24035..26053)
                     /gene="fadH"
                     /locus_tag="OHO85_001714"
     CDS             complement(24035..26053)
                     /gene="fadH"
                     /locus_tag="OHO85_001714"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCQ2671096.1"
                     /translation="MSYPSLFASLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSAFQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPSVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(26279..28630)
                     /gene="ygjK"
                     /locus_tag="OHO85_001715"
     CDS             complement(26279..28630)
                     /gene="ygjK"
                     /locus_tag="OHO85_001715"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCQ2671097.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDHLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(28647..29717)
                     /gene="ygjJ"
                     /locus_tag="OHO85_001716"
     CDS             complement(28647..29717)
                     /gene="ygjJ"
                     /locus_tag="OHO85_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HCQ2671098.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            complement(29851..31284)
                     /locus_tag="OHO85_001717"
     CDS             complement(29851..31284)
                     /locus_tag="OHO85_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCQ2671099.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(31347..31796)
                     /locus_tag="OHO85_001718"
     CDS             complement(31347..31796)
                     /locus_tag="OHO85_001718"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCQ2671100.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(31793..34885)
                     /gene="ebgA"
                     /locus_tag="OHO85_001719"
     CDS             complement(31793..34885)
                     /gene="ebgA"
                     /locus_tag="OHO85_001719"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCQ2671101.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLSASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            complement(35069..36052)
                     /gene="ebgR"
                     /locus_tag="OHO85_001720"
     CDS             complement(35069..36052)
                     /gene="ebgR"
                     /locus_tag="OHO85_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCQ2671102.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQLLAQEDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            36271..36603
                     /locus_tag="OHO85_001721"
     CDS             36271..36603
                     /locus_tag="OHO85_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCQ2671103.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            complement(36645..38024)
                     /gene="ygjG"
                     /locus_tag="OHO85_001722"
     CDS             complement(36645..38024)
                     /gene="ygjG"
                     /locus_tag="OHO85_001722"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCQ2671104.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            38442..39962
                     /gene="aer"
                     /locus_tag="OHO85_001723"
     CDS             38442..39962
                     /gene="aer"
                     /locus_tag="OHO85_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCQ2671105.1"
                     /translation="MSSHPYVTQQNTPLAGDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYILCALVMLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(40116..>40710)
                     /gene="nfeR"
                     /locus_tag="OHO85_001724"
                     /gene_synonym="yqjI"
     CDS             complement(40116..>40710)
                     /gene="nfeR"
                     /locus_tag="OHO85_001724"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCQ2671106.1"
                     /translation="HEGQPRHEGCCKGEKSEHEHCGHGHQREHGQCCGGRHGRGGGRR
                     QRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYPTLDFLQEQSL
                     ITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQNPQMKRALDN
                     FKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
BASE COUNT        10133 a        10314 c        10512 g         9751 t
ORIGIN      
        1 catccggcga gcagatttgt tttaaggttc cagtttcgga tctggtccaa agcggttttc
       61 gcctggcgta cccgcctggc agttaaagac gatgataata agccagccga taaacgggat
      121 taagaacaac agtgcccacc acgccgagcg gtcggtgtcg tgcaggcggc ggaactgaac
      181 tgcccaccac ggtaaaaaca ccagaatacc gtagattgtc gtcaggatgc cttcgccacc
      241 agcgcgttgc cagcctaaca ttttatccag cagccccagc acgaacgtaa agatgatgtt
      301 gaccagaata aacatccagt actctttgcg ccgcgcacga ccccggaaac cgacataatt
      361 ttttagtact ttcagatacc agtccatttt tgcctcaatt ttgctcaatc acttgttttt
      421 aaagcagcaa attaagaata gacgtgaatg aaatggcagt aaatattcgt tcaaggaatt
      481 aataaagtgc tgtgaaggcg cgctaagact gatgacaagc gcaaaatcgc cagatgcgct
      541 acgcttatct ggcctgcgcg gtcattgcaa catattgaat ataaatgttt tgtaggtttg
      601 ataaggcgtt cacgccgcat ccgacatgaa caacatgcac ttagctaatt atcagaggca
      661 cctcgtaagg tgcctttttt taaccgaagc gaacatctcg tccatgcggt tcatcgagat
      721 cctgccatgg accaattgaa ataatccccg tagggttgat ggtcttatgg ctgcggaagt
      781 aatgattacg gatgtgatcg aaattgactg tttcggcaat tcccggcatc tggtagatat
      841 cacgcaggaa gccatacaga ttaaggtaat cgctgatgcg gtgcttatca cacttgaagt
      901 gggtcacata caccggatca aaacgcacca gcgtggtcca caggcgaata tcggcttcgg
      961 ttagctgatt gccggtcagg taacgatgct ggcctaaaat ctgctccagt cgcgccagcg
     1021 attcaaacac tttcgccacc gcctcgtcgt aagcttcctg gctggtggca aaaccagctt
     1081 tatacacgcc gttgttaacg gtgtcataaa tccagccgtt aagttcgtca attttcgttt
     1141 gcagggctgg tgggtagtaa tcacccgctt tcgcgcccag cgtatcaaac gcggtattga
     1201 acatgcggat gatttctgct gattcgttgc tgacgatggt gtggttcttt ttgtcccaca
     1261 gcacgggaac agtaactcgt ccgctgtagt gtggatcggc gtggagataa agctgataca
     1321 gaaattcatt ttgatagagc gtatctccgg ttgctcccgg aaaactgtca tcaaaggtcc
     1381 agccgttttc cagcatcagc gggttcacta cggaaacgga aataaacggt tccagtcctt
     1441 tgagtttgcg catgatcagc gtgcggtgcg cccacgggca ggcgagtgaa acatagagat
     1501 gataacgatc tttctctgcg ataaaaccgc ctgtgccagt ggggccaggt gcgccatcgg
     1561 cagtgagcca gttacgaaat gcggaagctg aacgttgaaa tttaccgccg gtagatttgg
     1621 tatcgtacca ggtgtcatgc cagacgccgt caatcagttg acccattttt ctctcctcca
     1681 atagcaaaaa cgaggagctt cctcctcgtt ttttatcgtt aagtatagcg cgtgcttacc
     1741 actttttatt cagcaggcgg tcgatgctat acgcgcccgg accggtaatt gccagcagca
     1801 ggaatccgcc agaaattgtc aggtttttca tgaacatcag cgagttgacg ccttcagcaa
     1861 agttgctgtg aaataaaaat gccgtcagca gcgtaaagcc cgcagtaaac agggctgtgg
     1921 tgcgagtcag gaaaccgaac aggattgcca gaccaccacc gaactcaagc agaatcacca
     1981 gcggcagcat aaaacccggg acacccattg cttccatata ttgttgggta ccagagtaag
     2041 cggtgatttt tccccagcct gcggtaataa acagaatcgg cattaaaatg cgcgctacca
     2101 gtacaccaac atcttctaat tttttcatca tactctccac tgcgccactc aggctgctga
     2161 ttgttttttt gtgcaagggc gcggtattag cgtcgttgct gtcgatggag agaatcataa
     2221 acgtggtgaa tgatgattgt tagcaaggaa aactgtcaaa aatcttcaaa aaatttgagg
     2281 gataaggccg gaattgctcc ggccagaggg aagttaaccg cgaagctgtt gctgcttgag
     2341 ggtcgtttta accagacgcc aggcgctcca tacgccaaaa ccgcgtctgg cccagcggac
     2401 cagcatatta ggatggcgaa tcgtccagat cgccatcacg ctactgccaa ccagcgccca
     2461 ggagcgcaga cttagcagca tattccagcg acgatcgtaa gcgcctgttg cctccagcca
     2521 ttcacgacga ctggcggaaa gatccagccg ttgttgctgg atctggctaa gcagttgcgc
     2581 cttacgtcgt tcacgttcga ctttactgct cacgggactc ctcctcgagc agttgccgat
     2641 cgtttgccag ctcatggcgt gtatggcgca gcaaggtgga tttacgcgat ttacgtaacg
     2701 tccagatacc gccaattagt gccagtagca gcaacaccac ggtggtggca atcatcgcat
     2761 tcaggcgata ttgcggatca accgcccaaa taattagcac catcaggctc ataagaccaa
     2821 atgcagcgaa aagcatcgtc aggcccagca tcagtaaaag ttgaaagaga ttagcttttt
     2881 cctcttccag ctccaccacc gccagccgca gacgtgtttc caccatttca accatgatgg
     2941 aaacaattcg ctgcccgatg cccagaacgc ttttaccggg cccttgcgcg tgatgagtgt
     3001 ccgccataat taacgacgcg acagcagaac gccgagcact acaccgattg cagcgccaat
     3061 tcccacgccg gtccacggat tttcacgcac atactcatca gcacgcgccg ccgcgacacg
     3121 ggtttgtttg gcgatggcat caccggtttc acccaggcga taacggctct gtttcagtgc
     3181 ctgctccgct ttgctacgaa tcttactcaa ctcttctttc gacttctcgc cagatgagct
     3241 aagcacctct tccagcgtat cggaaaggga tttcaactca gcacgcagat gttccgtagt
     3301 gtgttctttc gacatagttt tctccaggtg agtaagtagt gactattgtg agttagtagt
     3361 cgcgcgcttc cagctttttc agctcttcct gcgcttctgc cagtttccgt tcgcgtttgg
     3421 caatcttatc ggcatcgcct ttttgcttcg cctcggctaa atcttgctgg cgttccgcca
     3481 cctcatcttt ctgctttgcg attttcttgt gatgatcggc acgcagctgg ctatctgaac
     3541 agttggcccg gacttcactc agggctttat tcagaccgtc aatacgattc tggttttggt
     3601 gtttttcggc atagctgatc tccttaagga tattttgctc cttttcctga cacagggtag
     3661 tggcataact accggcacta agagcaaaga gagaaacagc taaagcgatg cggtatttca
     3721 ttcttgaacc ttccattgtg attcggacct attacaaagg aacgattgtc cagacaacga
     3781 agcggtagtg ttaaccaccg cctcattaat catagacagt tacggtgaaa tcaccaaagt
     3841 gagtgatatg tttcggataa caggaagtta tccgaagcga tgagagttat cccgtaaccg
     3901 ggtcagccac tgcatagcct gactgttatt gtcctgctgc gcaatgatgt agccatgggg
     3961 caatgttctg tcgaggaccg cttttgcggc ggcgctctgg tcgctggagt caaatgtaat
     4021 taacagagtg tcgtttttgg gggtgatact tttgaaagga atgccatgag cgtcaaggtg
     4081 atgccagatc gaaaaaccgt ctggcatcgt tgtgccttga tgaacggcac gaatcgccag
     4141 cgtagactct tgctggcgaa ccgcggacca ggccagcaac agcgtgccga ccagaagtaa
     4201 aacagtgccg gaccaggcta gctggcgaag cgacatgcga ggtatttgca tcccttaccc
     4261 ccgatttcca tatttctttt tccataacac gaccagagaa cctgccaggc caaacaccag
     4321 cagcaccacc gggagcagca tcaggcatga catcagctgg tcctcgtact ttaaaaatac
     4381 cggcgttttg ccgagcatgt aacccagagt tgtcaggatc aatacccaca gcagaccgct
     4441 catccagttg aaaaactgaa agcgcgcgtt attcagccct gataacccgg caatcgtcgg
     4501 cagcagtgtt ctgacaaacg caataaagcg accaattaac agcgccgata aaccgtgttt
     4561 atgaaaaaga tggtgtgcgc gttgatgata atgcgcgggt aaatgagata gccagttttg
     4621 tacggtgcgg gtattgccca gccatcgccc ctgaatatag ctgacccagc agccgaggct
     4681 ggcggcaacg gtcagcagca gaatcgtttg cgggtaaccc atcgcgcctt tcgcaatcaa
     4741 cacgccgacc aataccagta aactgtcgcc cggtaaaaag gccgctggaa gcaagccgtt
     4801 ttcaaggaac aaaattacaa acaagacaaa atacaacatg ccaatcatcg atggattggc
     4861 cagggtttca aaatcctgcg cccacagggc ttgcagcaat tgggtcaaaa gttccattca
     4921 ttattcctgg tgaatcggtt aacgccacgc tggttttaaa agatgcacta ccgcaatgtt
     4981 attgctgttt tggtatgttc tgcgggtttg atacatcaac acagcctgaa ctattccctg
     5041 ttaaatcgtg agataagaaa gcattcaaaa acataaattc aaattgaatc taattgtaac
     5101 aaaggtcaac gttgttcgtt atcaaaatta catgtccgtg gagtctgatt ttgctgaatg
     5161 caggggaggg tggcgagagg atttacacag gctgacactt tatacttttc gcttacccac
     5221 gagacaaaca ggcgcgtcgc agcgcctgtc ggacaggtat ttcatttact gccactggtg
     5281 gccgtatcga gatgcaccac cgggttttcg gcgaatagat agcggtcggc attgaactcg
     5341 aagtcgtcgc tggtttcgtt gaataacatg atcttggtgt tttccaggtg ctgccacatt
     5401 gccagcttag cagcatgagg atctttgcga atcagcgcct tgaggatttg atcgtggtca
     5461 tcacaccagt tatcgacggt acgggaatca atgtgttcgt gcagtttttt ccagtacggg
     5521 ttatggctac gttgtgtcca cattttttca acgatggccg ccagggcgga gttctgcgtc
     5581 gccagggcta cctgaatgtg gaactgtaaa tcccactcgg aatcacggaa gcattgttcg
     5641 ccgcgcgcct gttcctgaat ggccatcagt ttcatgatgt cctgtttcgt tacctgagtt
     5701 gccgcgaatt cggcaatatt actttcgatg agctggcgag cctgaagcaa ctcaaacgga
     5761 ccgtaattgg cgaattccat attattgtca gctgcctgct gatggcgcgg ctggttggaa
     5821 accacatgaa tacctgaacc tttacgcact tcgacatagc cttcaacttc cagcatgatg
     5881 atggcttcac gaactaccgt acggctgacg ttcttttcat cggcaataaa gcgttctgca
     5941 ggcagtttat cacctaccag atagacgccc tgttcgatgc gctctttcag gtcagcggca
     6001 agttgttgat acaaacggcg tggttcagtg atttccatat gcgtctcccg gagtggaatg
     6061 cggacgaagt tatcatacca ctttattcat gggtatgcca ttggtcatat ataaatttgg
     6121 tgattgaatt aatgtattga attaatattg tgaatattta taaattccta aaaaataagt
     6181 atcgtcattg tgaaagctac taaatttcag taagttaatt cccagccttt aaatttaata
     6241 ttaactaaaa atgctttttt atattgactt acaataaatc tacatctcta atacaccaac
     6301 tttttggaaa caatattgta agatcaaatt tatatggtgg cgaaaattaa tctcttatca
     6361 cggcttacgc cgcttttatt cgtgttcgct ccttttttgg cacagtcaaa tatgactgtt
     6421 tatcctatgg ctgtttccat taatagtcag ggtgaaggca acgttcgcgt aatatctaaa
     6481 agtaatgaag ttcagtacat caaggcgaca gtattccgta tcgataatcc atcgacgcct
     6541 caggaaaatg aagttgagat taaatccggt gatgcaaatc atctggttgt tatgccacct
     6601 aaattcgctt taccagccgg tagtagcaaa accgtacgtt ttgttgcgat ggaaccagag
     6661 caaaaagaga aaaattatcg cgttaaattt gaagcggttc ccagtattga tgacgttgcc
     6721 acagataaaa aagatctctc tatgcagtta acagttaact taatttgggg gattgttgtt
     6781 agtgttccac ctcagcaacc tattgctaag ttagaagtaa atgctgccca aaaattagtt
     6841 aatgcaggga atcaacgctt aaagatttta acgatcgctt attgtaaaaa taatagcaaa
     6901 gaaaattgta agatacagac cgtaaataaa aacatcttcc ctggtcagga aagaaatctt
     6961 gaaagcatat ctggctacga caaaatcgtt gtcaaatata ataactggat caccaaagat
     7021 aatggcgagt ttgaactggc ggtccattaa ttatcattgt aataaacgaa ggaatagtaa
     7081 tgaaaaaggt atttgcaaaa tctcttctgg tcgcagcgat gttttctgtt gcaggctccg
     7141 cattggcagt acaaaaggat attactgtaa cggccaacgt tgatgccgct ctggatatga
     7201 cacagaccga taacaccgcg ttgccgaaag cagtagaaat gcaatatctg ccggggcagg
     7261 gtctgcaatc ttaccagttg atgaccaaaa tctggtctaa cgacgttacc aaagatgtaa
     7321 aaatgcagtt agtgtcccca gcgcaactgg ttcaaagcct ggatgctagc aagattgttc
     7381 cgctgacagt aacctggggc ggtgaagaaa ttaaagctga tgcggcaacc acttttaccg
     7441 caaccaaaat ctttgcttcc gatgcgttaa ctaacggttc tctggctaaa aacctgatgt
     7501 ttgctcaaac caccaaaggt gttctggaga caggcatcta ccgtggtgta gtaagcattt
     7561 atctgtctca ggatatctaa tccagataac agaattaaca cgtaacaaag ggaggggtag
     7621 ccctccctta ttttaactgt tttagtattt gttatggatt tcaggattgc catggataaa
     7681 aaattactgg ctcttttgat cctggcgagt ctcagcccgg cagaggcgac attaaccaaa
     7741 attcccgcag ggtttgaggt tattgctcag ggacagcagg agtatatcga ggtttatttt
     7801 tcagggaaaa gtctcggtaa atattatgca atggttaatc ttgataccgt aacatttctt
     7861 gatccagcaa gtctatataa caagctggag ctggatgtag acgatcagaa aatcgcgcat
     7921 atagtgaaag aaaaattatc gcagccgcta gctcgccacg gtgaattggc ttgcggttat
     7981 gtacgtactg actcagggtg tggttttctg aataccgata cgctggaaat aatctataat
     8041 gatgaagaaa gttcggcaac gttgtttatt aatccgcaat ggaattcagc tttcgatgcg
     8101 aagtcattat atttaaatcc agacaaaaat actgttaatg cttttataca tcagcaagac
     8161 atcaatgttc tggcacagga tgattaccaa tcgttgtcta ttcagggaaa cggtgcgctg
     8221 ggaataacag aaaatagcta tattggtgca cactggaatt tcaacggtta tgatgcagat
     8281 gatgtcagtg acagtaatgc tgacgtcagc gatctctatt atcgttatga ttttttacgt
     8341 cgttattatg tgcaggcggg tcgcatggac aaccgcacac tatttaatgc acaaggcggg
     8401 aactttacct ttaactttct gccactcggt gcaattgatg ggatgcgtat cgggtcgaca
     8461 ctgagctatt taaaccaggc gcaaagccag caaggaaccc cagtaatggt tctgctttcg
     8521 cgcaattctc gtgttgacgc ttatcgtaat gagcaacttt tgggatcgtt ttatctcaat
     8581 agtggttcgc aatttattga taccagttcc tttccgcctg gcagctacag cgtggcatta
     8641 aaagtctacg aaaataacca actcacccgc accgagcttg tgccgtttac caaaaccggc
     8701 ggtctgactg acggaaatgc gcaatggttc ttacaggcag gtaaaactac atcacaggtt
     8761 tctgatgatg aaagttcagc ttatcagcta ggagtacgcc tgccattaca tccgcaatat
     8821 gagctctacg cagggctggc gaatgccgat gatgtgagtg ctttcgagtt aggtaataac
     8881 tggacggcag atttaggcgg ggtggggaat cttgcaatca gcgccagcgt gttccgtaac
     8941 gatgacggcg gcaaaggtga tatgcaacag gccaactgga gtaatccggg atggccgacg
     9001 ttgggctttt atcggaccaa ctctgacggc gatgcttgta caaccgacag cagagagagc
     9061 tataacgcct taagctgtta tgaaagtatt tccgcgacgg tttcacagaa ttttgtcggc
     9121 tggaatatga tgctgggtta tacccgcaca caaaataaca ctgatgatag tttgcgttgg
     9181 gataaacagc agagctttga aaataactat cttcgccaga caacagcgca aagtatctct
     9241 gaaactgtac aacttagcgc ttcccgcgct tttgtgatgc gtgactggat tttgagtacg
     9301 tcagtcggtg ttttccatcg taatgacaac ggtggcgata acgacgacaa cggcttgtac
     9361 ttatcgtttt cgttatctga cacgccaacg atggatagca ataacaacag ccattcaaca
     9421 aatgtgtcta cggattatcg ttatagcgaa caggatggcg atcaaacgtc atggcagtta
     9481 tcgcatacct tttataacga ttcattcagc cataaagaac ttggcgtaac cgtcggaggc
     9541 ctgaacaccg ataccataaa cagcgcggtt aacgggcgtt gggatgggca atacggaaat
     9601 gtctacgcta ccgtatctga tagttatgac cgtaagaatc atgatcatct ttcggccttt
     9661 acgggcactt atagctctac gctggctgtc agtcgctatg gcgttaattt gggagccagt
     9721 ggtacagacg atttgctggg tgcggtactg gtggatgtga aaggcttctc tgaacaggat
     9781 gaagagagtc aggatctgca actcgaagcg cgggtggcag gcagccgaac tttgcagctt
     9841 ggtcaaagtg acagtgtgtt gttcccttat cctggatttc agtctggttt tgttgaggtt
     9901 aacgacagta gccagggcaa tcagcagggg acaacaaaca tcattaacgg tgcagggaat
     9961 cgtgaattaa tgttgttgcc tggcaagctg cgctatcgcg aagtgtctgc cagctttaac
    10021 tacaactata tcggtcgttt attactgccg gcagcggtga aaaaattccc gattgtgggg
    10081 ctgaacagcg ccatgttact ggtagctgaa gatggcggat ttacacttga aattaacggc
    10141 agcgaaaaag agctctatct gctttccggg cagcaattcc ttaagtgtcc actaagtgtt
    10201 gtaaagaaac gcgccagcat tcgttacagc ggagatgtta cttgtagtgt ggtgacttat
    10261 tcacaattac cggagtcgat tcaggttcag gcacagttga aacagcctaa attacgtgga
    10321 aacgttcaga cggcgcaaag ggaggttgca ccatgagaaa tcgattgatt gcggcgatat
    10381 tgggcttgtt tggcacgctc actggcgttc aggcagctcc tgacgtgacc agtgaaatta
    10441 cgtatgattt ggcatctggt agagcggatt attacttctg gaaggatgaa gcgtcggcag
    10501 gaaataatgg atatatgtgg tatgaatgtt cgtatcctga cctccaacaa acctgtacag
    10561 ctaatggaaa tatatcgaca gtacaaatct atttaactga acaacgcagt gggatgcgtt
    10621 ggccggtaaa actcaaagga tttaaaacag ccattgtaag tagcgatgaa gcgccgccag
    10681 gatgcaaggg gggcaaaggg cttcagacga atcttaagga ttctaataga tcttcatgta
    10741 cagaagatgg tcaacattat tatatatacg atacaaagtt tcttacgctc taccttgagc
    10801 agacagagat gaagaatttg ccgattggtg gcgtctggaa ggggaaagtt aaattacatt
    10861 cgaacagccc ggcccaggac tatttcgcaa atattaccct gaatacgctc gaccccaacc
    10921 atattgacgt gttcttcccg gagttcgccc acgccacgcc aagggtgcag ttagacttgc
    10981 atccaacagg aagcgttaac ggcagcaact acgcgcaaga tctgaccatg ttggacatgt
    11041 gcctgtacga tggttttaac ggtaatgcca tcagttatga aatcatgctc aaagatgaag
    11101 ggcgaccagc tgcagggcgc agagacggtt acttctctat ctatcgtcag ggagggacca
    11161 ccaccgacga gggagaacgc attgattacc gggtcaaaat gtacaacccg gaaaccggtg
    11221 ggcaaattga tgtgcgcaat aatgaaaata tggtctggaa cagcattaac ctgaaacgtg
    11281 tgcgtccggt ggtactgccc ggtattcgct atgccgtgat gtgtgtgccg acacccttga
    11341 ctctggcagt cgataaattc agcgtgatgg ataaacaggc cgggtactac atgggcaaat
    11401 tgtcagtaat ctttacgcct tccttgccaa ccatcaatta accctgcaaa gcggacaaac
    11461 gcttttttct atataaacga cgaagccgtc tgataaagcg gcttcgtcat tacaacttaa
    11521 gttaaacgtt aatgttgcgg tgcgggatcg ttatgtgttt cctgcgccac ctcaatcgcc
    11581 ggtttgttct gcaacacggt ccagataacc agcgcaccta acaggtcaaa tactgccaga
    11641 actgcgaaca gcgggctgaa gccgatggtg tcagccagtg caccgacaac cagcgcaaac
    11701 agcgtacttg ccagccatgc ggacatcccg gttaaaccgt tcgccgtcgc cacttcgtta
    11761 cgaccgaaca cgtcagaaga gagcgtaatc agcgcgccag acagtgcctg gtgggcaaaa
    11821 ccaccgatac acagcagcat aattgcgaca tacgggttgg tgaacagacc gatcataccc
    11881 gggccaatca tcagcacagc acccagcgtt acgaccatct tacgggaaac gatcaggttc
    11941 acaccaaacc aacgctggaa cagcggcggc aggtaaccac cgaggataca accgaggtca
    12001 gcaaacagca tcggcatcca ggcgaacatc gcgatctctt tcaggttaaa gccgtaaact
    12061 ttaaacatga acagcgggat ccacgcgtta aaagtacccc aggccggttc tgccaggaaa
    12121 cgcggcagcg cgatacccca gaactgacgg ttacgcagga tctgaccaac ggacattttc
    12181 ttcgccgtgc tcacctggtg ctgggcttcc tgaccattaa taatatagtc gcgttcttca
    12241 tcggtcagat gcttctggtc gcgcggatgt ttatagaaaa tcagccatgc catcgcccag
    12301 ataaagctca atgcaccgga gatgataaat gccatctgcc agctgtgcat tacgattgcc
    12361 cataccacca gcggcggcgc aatcatcgca ccaatcgaag aacctacgtt aaagtaacct
    12421 accgcgatgg aacgctcttt cgccggaaac cattcggagc tggctttcag acccgccggg
    12481 atcatcgctg cttccgcggc accgactgca ccacgagcaa tcgccaggcc accccagcta
    12541 ccagccagcg cggttgcacc acagaatacg gcccacagca cagcaaacat tgcataaccg
    12601 attttcgtac ccagcacatc cagtacatag cccgctaccg gttgcatgac cgtataagca
    12661 gcagaatagg ctgcgatgat ataggaatac tgttgggtgg agatgtttaa ctcttccatc
    12721 agagttggcg cagctgccgc cacagtgtta cgcgtcaggt aaccaagcac ggtgccaagc
    12781 gtcaccagtg cgatcatata ccaacgtaac cctttaattt tacgcatgta aaacctcatc
    12841 gttttgttat atccgcagga agcgcggcca tcatcccgcc agaggctgaa gatgttagtt
    12901 acgaccgggt aaccgaaaaa aaaccggcac gccccgaacg ttgccatgaa agtttcgtgc
    12961 taattcggct tccgtaccgg ttaacccgat tcatctaaca aatgaatgaa tcggcaattt
    13021 gcattccgtc gacttatgat ttgcgacggc agaaagataa cttgtcatac aactttaaaa
    13081 ggtgagagcc atcacaaatg tgcgaatatt tgtagggaca ttacctgacg acagcgaggc
    13141 cagtactggc gcggcctgca gcgagattta ccactttgag agtaattttt ttaactacgt
    13201 ttattgatct aactcacgaa aatatcttcg gactctggaa attggtgtga taactttgtc
    13261 agcatcgcac cataagcaag ctagctcact cgttgagagg aagacgaaaa tgactccgtt
    13321 tatgactgaa gatttcctgt tagataccga atttgcccgc cgtctgtatc acgactacgc
    13381 aaaagaccag ccgattttcg attaccattg ccatttgccg ccgcagcaga ttgcggaaga
    13441 ctatcgtttt aaaaacctgt atgacatctg gctgaaaggc gatcactaca aatggcgcgc
    13501 tatgcgtacc aacggtgtgg ctgagcgtct gtgtactggt gatgcgtctg accgtgaaaa
    13561 atttgacgcc tgggcggcga ctgttccgca cactatcggc aacccgttat accactggac
    13621 gcacctcgaa ctgcgtcgtc cgttcggtat cactggcaaa ttgctttctc cgtcaactgc
    13681 cgatgaaatc tggaacgaat gtaacgaact gctggcgcag gataacttct ccgcgcgcgg
    13741 catcatgcag cagatgaacg tgaaaatggt cggcaccacc gatgacccga tcgattctct
    13801 ggagcatcac gcagagatcg ccaaagacgg ctctttcacc atcaaagtgc tgccgagctg
    13861 gcgtccggac aaagccttta acattgaaca ggcgaccttt aacgactaca tggcgaagct
    13921 gggcgaagtt tccgataccg acattcgccg ctttgctgac ctgcaaactg ccttgaccaa
    13981 acgtctggat cacttcgccg ctcacggctg taaagtgtct gaccatgcgc tggacgtggt
    14041 gatgtttgct gaagcgaacg aagcggaact ggacagcatt ctggcgcgcc gtctggctgg
    14101 cgaacccctg agcgagcacg aagtggcaca gttcaaaact gcggtactgg tgttccttgg
    14161 tgccgaatat gcacgtcgcg gctgggtaca gcagtaccac attggcgcgc tgcgtaataa
    14221 caacctgcgt cagttcaaac tgctggggcc ggatgtaggc tttgactcca tcaacgaccg
    14281 tccgatggcg gaagagttgt ctaagctgct gagcaagcag aacgaagaaa acctgctgcc
    14341 gaaaaccatt ctctactgcc tgaacccgcg cgataacgaa gtgctgggca ccatgatcgg
    14401 taacttccag ggcgaaggta tgccgggcaa aatgcagttc ggttccggct ggtggtttaa
    14461 cgatcagaaa gacggtatgg aacgtcagat gacccaactg gcgcagctcg gtttgctgag
    14521 ccgctttgtc ggtatgctga ctgacagccg tagcttcctg tcatacaccc gtcacgaata
    14581 cttccgccgc attctgtgcc agatgatcgg tcgctgggtg gaagcgggcg aagcaccggc
    14641 ggacatcaac ctgctgggcg agatggtgaa aaatatttgc tttaacaatg cgcgtgacta
    14701 cttcgccatt gaactgaact aaggtctggg gttgatatgc aatacatcaa gatccatgcg
    14761 ctggataacg tcgcggtcgc tttagcagat ttggctgaag gcacagaagt cagtgtcgat
    14821 aaccagactg ttacgctgcg ccaggatgtt gctcgtggac ataaatttgc gttaacggat
    14881 atcgcaaaag gggccaatgt catcaaatat ggcctgccga ttggttatgc attggcggat
    14941 attgcggcgg gggaacacgt ccacgcccac aatacgcgca cgaatctgag cgatctggat
    15001 cagtatcgct atcaacctga ttttcaggat ctgcctgcgc aagcggcaga tcgtgaagtg
    15061 cagatctatc gtcgcgctaa cggcgatgtc ggggtgcgta acgagctgtg gatcctgcca
    15121 accgtgggct gtgtcaacgg catcgcgcgg caaatccaga accgtttcct gaaagagacc
    15181 aacaacgccg aaggtaccga cggcgtgttc ctcttcagcc acacctacgg ctgctcacaa
    15241 ctgggcgacg atcacatcaa cacccgcacc atgctgcaaa acatggtgcg ccacccgaac
    15301 gcgggcgcag tgctggtgat tggtctgggc tgtgaaaaca accaggttgc cgcattccgt
    15361 gaaacgttgg gcgatatcga tcctgaacgc gttcatttca tgatctgcca acagcaggat
    15421 gatgagatcg aagccggaat cgagcatttg catcagctgt ataacgtgat gcgcaacgat
    15481 aaacgcgagc caggcaaact cagcgaactg aagtttggtc tggagtgcgg tggttctgac
    15541 ggtctttctg gtattactgc taacccgatg ctggggcgtt tctctgacta cgtgattgct
    15601 aacggcggta ctaccgtact gaccgaagtg ccggagatgt ttggcgcaga gcagttgctg
    15661 atggaccatt gccgcgacga agcaacgttt gaaaaactgg tcaccatggt caacgacttc
    15721 aaacagtact ttattgccca tgaccagccg atctatgaaa acccatcgcc ggggaacaaa
    15781 gcgggcggta tcaccacgct ggaagataaa tcccttggct gtacccagaa agcgggttcc
    15841 agcgtcgtgg ttgacgtgct gcgttacggc gagcgtctga aaacgccggg gctgaacttg
    15901 ttaagtgcgc cgggtaacga tgccgtagcg accagcgccc tggcgggtgc gggctgccat
    15961 atggtgctgt tcagtactgg tcgtggcacg ccatacggcg gctttgtacc gacggtgaaa
    16021 atcgctacca acagtgaact ggcggcgaag aaaaaacact ggatcgactt tgacgcgggt
    16081 cagctgatcc acggtaaagc gatgccgcag ttgctggaag aatttatcga caccatcgtt
    16141 gagtttgcca acggtaaaca aacctgcaac gagcgtaacg acttccgcga actggcgatc
    16201 ttcaaaagcg gcgtaacgct ataaagtcga aatgagttgt taataaaaaa cggcgtttca
    16261 taatgtgaag cgccgtttgc ttttccatac ctgctaagga tttatcatga ccgcgtattg
    16321 gctggcccag ggcgtgggtg tcatcgcctt tctgattggt atcacaacat ttttcaatcg
    16381 tgacgaacgt cgcttcaaaa agcagctttc ggtctatagc gccgttattg gcgtacattt
    16441 ttttcttctg ggcacctatc ccgctggtgc cagtgccatc cttaatgcca ttcgtacatt
    16501 gattacctta cgcacgcgca gcttatgggt aatggcgatt tttattgtgc tgactggcgg
    16561 aattggcctc gcgaagttcc atcatcctgt cgaactattg ccggttatcg gtacgattgt
    16621 cagtacctgg gcgctgttcc gctgtaaagg gctcaccatg cgctgcgtga tgtggttttc
    16681 aacgtgttgc tgggtgattc acaacttctg ggcggggtcg ataggcggca caatgattga
    16741 ggttagtttt ctgcttatga atggcctgaa tatcattcgt ttctggcgga tgcagaaaag
    16801 gggaattgat ccgtttaaag tagagaaaac ccctcccgcc gtagacgaaa ggggttaaac
    16861 aattaattac gcagggcgct atttgccaga cgatcgtctt ccgcctggca agctgccgca
    16921 gtgaacagca cgtcagttga agagttcagc gcggtttcgc aagagtcctg caatacgccg
    16981 atgataaagc cgacggcaac cacctgcatg gcgatatcgt tcgaaatacc gaacatatta
    17041 caggccagcg ggatcagcag cagagaaccc cccgccacgc cggatgcgcc acaggcacac
    17101 agagaagcca ccacgctcaa cagcagcgcc gtgggcagat cgaccgggat acccagcgta
    17161 ttaaccgcag ccagcgtcaa cacggtaatg gtgattgctg cgcccgccat attgatggtg
    17221 gcacccagcg gaatagaaac ggaataggta tcgcgatcca gattcagctt ttcacacagt
    17281 gccatattca ccggaatgtt cgccgcagag ctgcgggtga agaaggcata cacgccgctt
    17341 tcgcgcaggc acagcagcac cagcgggaac gggttacgac gaattttcca ccacaccagc
    17401 aatgggttaa ccaccagcgc caccagtaac atacagccaa ccagcacgac cagcagttgc
    17461 gcgtagcccc acagtgtgga gaaaccggtg gttgccaggg tagaagaaac cagcccaaaa
    17521 ataccaatcg gtgcgaagcg aatgaccagt ttcaccataa aggtaacggc attcgacata
    17581 tcgttaacca ggtttttggt ggtctcgtta ccgtgacgca gtgcgaagcc gaggccgatc
    17641 gcccacacca gaatcccgat gtagttacct ttcagcagcg cgtcgatggg gttggaaacc
    17701 atgctcatta ccagcccgcg catcacttcg acaatgcctg acggcggcga aatatcaccc
    17761 gcgctactgg ataaatgcag ggtagaaggg aaggcaaagc tgaagactac tgcggccaga
    17821 gcagcagaga aggtgcccag tagatagagg aacaaaatag ggcggatatt ggttttctgc
    17881 ccgtgctggt ggttagcaat agatgccatt accagcatca acaccaggat gggggcaacg
    17941 gctttcagtg cgccgacgaa caaagtacct aacagaccaa cagcttccgc cgcgggtttt
    18001 gagatccatg ccagaagaat ccccagaaca aggccgacca ggatttgttt taccaggctg
    18061 ccatgagcca gacgccggaa tagccccggt gaacgttgcg tagtcatttt tcgatccttt
    18121 cattgtgttg tcgcacatcc ctggtgcgtt ctgtcgacgc atctttcagg atgtaacaaa
    18181 gtgtttgcaa gatcgagtat aaggaaagtt acagcttcgg gaagcgaaaa atgctggatt
    18241 ttaaagagag gtcatatttt gcaacttaat ggttacattt atttgacata aaaataaagc
    18301 caacgtgcgg cttccagact gatgacaaca tcaaaaaagg actcggtcag tcccctcgcc
    18361 ccgtttgggg agagggttag ggtgagggga acaggccagc atcggtgcga aaaattaccc
    18421 tcaccccgac cctctccctg gaagggagag ggagaaaacg gtgctcatgt taatgacagg
    18481 gtacacggct taggcagatt aaaaattatc caccccgctg cttatcatgc cgataattca
    18541 cccaggcgtt gataataaac gtcatcacca gaatgccaaa caccacgccc agcgagacgg
    18601 cgattggaat atggtagaag tcgacaatca gcatcttgat accgataaac accaggatca
    18661 ccgccaggcc atatttgagc atcgagaaac gctctgctac gcccgccagc aggaaataca
    18721 tcgcacgcag gccgaggatc gcaaacaggt ttgaggtcag cacaatgaac gggtcagtgg
    18781 tcacggcgaa gatagccgga atgctatcca cggcgaaaat cacgtcgctc aattccacca
    18841 gaatcagtac cagcatcagc ggtgtggcgt acagcaatcc attcttacgc acaaagaaat
    18901 gctcgttgtc gatggtgtcg gtcatgcgca aatgaccgcg tagccagcgc accagcggct
    18961 tgtcgccaat gcctgattca tcttcatggg cgagcgccat cttcacgccg gtaaacagca
    19021 ggaaggcacc gaagatatac aggatccagt cgaactgcga aattagccag ctgccagtga
    19081 agatcatgat ggtacgcaga acaatcgccc cgagcacgcc atacaccagc acgcggcgtt
    19141 gtaatgccgc cggaacagag aaatagctga acaacatcag ccagacaaag acgttatcga
    19201 ccgccagcga tttctcaatc agataacctg tgagaaaggc cagtgcctgt ggatcggcaa
    19261 cggcgcgacc ttcggtttgc accagatacc accagaaagc ggcgttaaac agtaacgaca
    19321 gcgtcaccca gaccagcgac caggccgcag cctgtttcat ggtcatggca tgtgccccac
    19381 gacgcccctg caacaacagg tcgatagcca gcataatggc gacaacaaca gcgaatccgc
    19441 cccatagcaa cggcgtgccg acagtattca tagaagttcc ttacacataa aaaaacggcc
    19501 aacgtcggaa gacgtcagcc gctgcttttt atgcatagac ctcgccttcc ggcaaggtct
    19561 cacttacaac aaaaaaggaa tacgtctgcg tattcctttc gggttgcccg gtgaccggat
    19621 gtggtttttc acacatcgta atgacgatcg accggcaatg aagttactcc cctttgcagg
    19681 taacaaagta tgacagacca ttcagccagt caatggtctg ttacatacct tttacactgt
    19741 tttactttgg caattatagt tttacgctat ctgcccgaaa aatcacccca gtctggcggc
    19801 ggatctcggt cagcagtttg gcggtgatgc gactgaccgc cagccccgga tgatccacca
    19861 gatgctcatc caccagggta gcgaacagct ctgcttcgta gagcatggta ttaatatgct
    19921 gcggctgggt gagatcctgc atttggctgc cacgcggcac gaagcacact ttctggcatt
    19981 cagacagttt ttcaatcacc agcgatcctg cttcgccctg aatctcgctc gccaggacag
    20041 aatcactgac ttttgagtgc tgcaaggtga cgctgaaatc accgtaatcc atcaccacca
    20101 cgccgtgggc atctacaccg cttgccagca aactggcggt tgcctgcacg cttttcggct
    20161 caccaaataa cgccaccgcc gacgccaggc agtaaaagcc gatatccata attgaaccgt
    20221 tagagaatga cggattaaag gtgttgggat tttcaccgtc caggtaacgt tgatagcgcg
    20281 aggaatactg gcaatagttg aaaaagactt tacgcaattt gccgactttc ggcagcgcct
    20341 ggcgcagcaa atgaaagttc ggcaggcagg cggttttaaa tgcctcaaac agcaccacct
    20401 gattttcccg cgcacaggca atggcggcat ccacttccgc cagattcgac gccagtggtt
    20461 tctcgcaaat cacatgaatt ttatggctaa ggaaaagttg tgtctgggaa aagtgcaggg
    20521 cattcgggct ggcaatatac accgcgtcaa tggcatcgct ttccgccatc gcttccagcg
    20581 aggtaaacag atgctcgaca gaaaaatcat tggcgaagtg ctgagcctgc tcaaggctgc
    20641 gggaatatac ggcggttaac ttgtatttac cgctctcatg ggcggcctcg acgaactggc
    20701 gagtgatcca gttcgtacca atcacagcga aacgtatcat aacgcgtgga tactccataa
    20761 agggattctg tcgtcaatgt agcacgcctt tttcatcttc gacgtgcgca gatacgcatt
    20821 attcttttgc cggacagcca tgctcactaa acggcccaat catgaccgat ggcccgagat
    20881 atttcggctg gcgatggagg attttggcgt caatcacggc gctgacacga gcgagtttct
    20941 cccgcagttg agtataaaaa ccgtgcttca tattgaggtc aggcgcgtta aaaccgatag
    21001 cgtcgatgcc gtactgtttt gccagccaga tggcgcgttg attatgaaat tcttgcgaga
    21061 taatggtgat atggttttca ccaaaaacct ttttggcacg cactaccgaa tccagcgttg
    21121 agaatccggc atagtcgcag aagatcactt tcgccggtac gcctttagcg atcaaagcct
    21181 gctgcatacc tgaggcttca tcgtaatttt tacgtccgtt atcgccactt accagtagcc
    21241 atttcacttt tccggcgtgg tacaacgctg ctgcggtatc aatacgtcgg gtaaagtagc
    21301 gattacccgg cctggccccc agcaataagc ccacgttacg cgccggaact gcgttgacat
    21361 cgctccaggt cagctgcttg ctggcgttga ccataacgcg atcggcaata aaaatggtcg
    21421 ctcctgctat aagcaacaac aaacagccaa tcttaagagt ccggcgagaa aaacaaatac
    21481 ggagaagaag gcggggctgc atggtcgtta tccttaacga aaggggggag gtcgatttta
    21541 ggcgctaaag aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc
    21601 gacaactgcg ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata
    21661 tggcagcaca actggtaaaa cgctttgtta tgctctttct ccttcaggtg cgccagttcg
    21721 tgcaccacga tcatacgtaa aaacggttcc ggcgcgttgc gaaacacagt agcgacgcgg
    21781 atctctgctt ttgctttcag cttgccgccc tgcacacgag aaacagcggt atgtaacccg
    21841 agcgcgtttt ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca
    21901 ttgcgcagaa actgattttt tagatcctga gtatattgcc agagggcttt atcggtggcg
    21961 tagtcgtgtg ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc
    22021 agcgttcgca cctgggaaag tagctgctcg ggataaccct gaagataagt aagattgctc
    22081 ataaccgccc acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt
    22141 cgaaatttta tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga
    22201 cactacaacg ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcgg
    22261 atgaatattt gctgcaacag ttggacgaca cagaaatccg cggcccggtg ttgatcctga
    22321 atgatgcctt tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg
    22381 actcatacat cagtgaactg gcgacgcgcg agaatttacg cctcaacggg attgatgagt
    22441 cgagcgtgaa gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga
    22501 tcaaagtgcc gaaaacactg gcattgctgg aacagcaact gcgtgcgctg cgcaaagtgg
    22561 tcacgtcgga tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc
    22621 tggagctgtt cgagaaagtg ctcggcccga ccaccactac gctggcatgg aaaaaagcgc
    22681 gcctgattaa ttgcactttc aatgaaccgc cgctggccga tgcgccgcag accgttagct
    22741 ggaagctgga aggtactgac tggactatcc acaaccatgc gaatgtcttc tcccgcaccg
    22801 ggctggatat tggcgcacgc ttctttatgc aacatctgcc agagaatctc gaaggtgaga
    22861 ttgtcgatct cggctgtggt aacggcgtta ttggcctgac gctgcttgat aaaaacccgc
    22921 aggcgaaagt ggtgtttgtc gatgaatcgc cgatggcggt cgcttccagc cgaatgaatg
    22981 ttgaaaccaa catgccagag gcgttggatc gctgcgagtt tatgatcaac aacgcgctct
    23041 ccggagtgga gcctttccgc tttaatgctg tgctctgcaa cccgccgttt catcagcaac
    23101 atgcgctgac cgataacgtc gcctgggaga tgttccacca cgcccgccgc tgcctgaaaa
    23161 tcaacggcga actgtatatc gttgccaacc gtcacctgga ttacttccat aaactgaaga
    23221 agattttcgg caactgcacc accatcgcca cgaataataa atttgtggtg ctgaaaacag
    23281 tgaagctggg gcgtcgtcgg taagattggg agttacctgc cggatgcggc gttaacgcct
    23341 tatccggcct acgttcagca cgttactccc aggccggata agacgcggca agcgtcgcat
    23401 ccggcaatgt ctgcccagtt ctggaatcgc gcttccataa aacaaaaaat ccctgtcacc
    23461 atcctctaca ttctcttgtt tagcgttgtt caacgtttat gctacatacg tgaagttatg
    23521 acgcataaag aatacgattt ctttaccgct gttcatcgta ctaaggggaa aaaatgattg
    23581 cgattgccga catcttgcaa gcaggagaaa agctaactgc ggtggcacct tttctggcgg
    23641 gtattcagaa cgaggaacaa tacacccagg cgctggaact ggtagatcat ctgctgctca
    23701 acgatcctga aaaccccttg ctggatctgg tgtgtgccaa aataaccgcg tgggaagaat
    23761 cagcgcccga atttgcggaa tttaatgcca tggctcaagc catgcctggc ggtatagccg
    23821 tgattcgtac ccttatggat caatatggtt taaccctttc cgatctgccg gaaattggca
    23881 gtaaatctat ggtgtcacgc gttttgagcg ggaagaggaa attaacgctg gaacacgcta
    23941 aaaaattggc aacgcgattc ggcatttctc ccgccttgtt tattgattaa cgcgacgtgc
    24001 cggatgcgtt gcatccggcg cttcaaataa cgatttaaat ctccagcgcc agccgtgttc
    24061 cctgggcaat tgcccgtcgt gcgtccagct ccatagccac atcgcagccg ccaattaaat
    24121 gcacagtttt cccgctatca atcagcggtt gcgccagcgc gcggtttggc tcttgccctg
    24181 cgcagatcac cacattgtcc actgctaata cctgcgtttc gccgttgatc accacatgca
    24241 gcccgtcatc gtcaatcttc tgataactta cgcttgggat cattttcaca ccccgcgaga
    24301 gcagggtggt gcgatggatc cagccggtgg ttttgcctaa cccctgtccc ggtttgctgg
    24361 ctttgcgctg gagcatcaca atttgccgtg ggctacgcgg gatctgcatt ccctgcgggc
    24421 ttaagccacc agcctgttgt aggctgctgt cgatccccca ctcattacag aacctggcga
    24481 tattctggct ggtggattcg cccggctgac ttaaatacat cgccgtatca aaaccaatcc
    24541 cgccacaacc gatgatggca accttgttgc ctaccggcgc tttgtcgcgc agtacatcga
    24601 gataactcaa taccttcgga tgatcgatcc cgtcgatagg cggaacgcgc ggcacgatcc
    24661 cactggcgag gatcgtttca tcgaacgcct gtaactgatc cgccgtcacg gtgtgattga
    24721 gtttcagcgt cacgcccgtc acttcgatca tccggtggta atagcgcagc gtttcgtaaa
    24781 actcctcttt gccggggatc tgtttggcga tattaaactg cccgccgatc tcgctgtggg
    24841 catcaaacaa tgttacctga tgcccacgcg ccgccgcgtt aatggcaaac gccagcccag
    24901 caggtcccgc accgaccacc gccagatttt ttttctgcac ggcaggaagg attggcattt
    24961 tggtttcgtg gcaggcgcga ggattcacca ggcacgaggt gactttgcca acgaagatct
    25021 gatcgaggca ggcctgattg cagccaatgc aggtgttgat ctcatcggct cgccccgatt
    25081 gcgcttttga cagcagttcc gcatcagcaa gaaacggtcg tgccatcgac accatatcgg
    25141 catcgccgcg cgagagaatg tcgtcggcaa cctgcggatc gttaatccgg ttggtggtca
    25201 ccagcggcag cgagacgtgg cctttcagtt tgcgcgtgac ccaagtaaat gcgccgcgcg
    25261 gcacgggcgt ggcaatggtc ggaatacgtg cttcatgcca gccaatgccg gtgttgataa
    25321 tggtcgcgcc cgccgcttca atggcctgcg ccagttctac cgtttcggca aaagtgccgc
    25381 cgccttcgac caggtcgagc atcgacaacc ggtagataat aatgaagtcg ttgccgacgc
    25441 gttcgcgcac cgcacgcact acttctacgg caaatcgcat ccggttgcgg taatcgccgc
    25501 cccactggtc actacgctga ttggtgcgca gcgtcagaaa ttcgttgatc aaataccctt
    25561 cggaacccat cacctctaca ccgtcgtatc ctgcctcccg cgccagttgc gcgcagcggg
    25621 cgaaatcgtc gatcagttgc aggatctctt catgggtgag ttcgtgagga acgaaacggt
    25681 tgatgggggc ctgaaatgcg gagggagcga ccagatgcgg ttggtagctg tagcgcccgg
    25741 tatgcaaaat ttgtaaggca attttgccgc cttcctgatg tacggcttcg gtaatggtgc
    25801 gatggtgtgg gatctggctg gcgtcattga gcattgcgcc accttccatg ccgacgcctg
    25861 ttaaatctgg tgcaatgccg ccgctaacaa tcagcgccac gccgtgacgg gcgcgttcgg
    25921 cataaaacgc agccagccgt tcagcaccgt ccgggtattc ctccagcccg gtgtgcattg
    25981 aacccatcaa cacgcggttt tttaacgtgg taaaacctaa atccagcgag gcgaacagcg
    26041 acgggtagct cataaaattg tccagtatgt aaaataattg ttatgtggtc ggatgagttc
    26101 taatttagcc gtcgccggag aaaagggaaa aggggagtgc ggggtttgtg atgggattca
    26161 aaaagaatac gcccggtggt aacgccggat gcgacgcttg ccgtgtttta tccggcctgg
    26221 gggattgcac atgtagggcg gataaggcgt ttacgccgca tccgccagca gttgcaaatt
    26281 attgcttacg gaaaaaatcg ttatacaaca tatacaaatg cgcggcactc caggagaaat
    26341 ttggtgcgcc ttgctgtgcg cctgtcagcg ggttgtaatt ttcctgaatt ggtccatcgg
    26401 cggttaaccc tttggcgtgc cggaagaacg tatccgccag cttcagggca tcatcacgat
    26461 aaccgtaacg ctccatccct ttcagaccaa accagaactg atccacccat acgcgcccac
    26521 gccagtagat atcagcgcca aaagccggat tggttaacgc cgccgttccc agcgggacaa
    26581 aggtgttgaa ctctttagga tcgagcatca ccttcaccac cgcgtcggcg ttggcctgcg
    26641 ttgccgcacc gttaaacagc ggcgaccagc cttccggccc tttaccgcgc tcaacaatcg
    26701 gtttgcccgc gcagccgttc gccagcggtt tatcttcaat acgcacgtca tagtagaact
    26761 gggtagtcgg gtcgaacata caggtgttga tgtagtccgc gagctgctgt gccaactggc
    26821 gatagcgttt ggcctcttct ggcttaccga ggatggttgc catctccgcc agataatggt
    26881 tatcgctgta catatagctg gcctgatcca ccgactcctg caatagcgag tagcccagca
    26941 acgttccgtc ctgactgcgg ttttcggcga atttcaccgt ccagtcgcta cgtttgccgc
    27001 cattagcgac atatttatcc aactgttctt tgtcgataaa cccaaagacg gcggcatcat
    27061 cacgccccga ttcccacgat gcagcaacct gtgccggaat ttccagactg tcgtactggc
    27121 ctttctccac cacgcgggcg tagttgttca gcccagactg cgtctcttct ttatcgcctt
    27181 ttttcaccgt aaacagcatc tcgccgctct cagtgttgtg ggctttgtcg cgggtcgcgc
    27241 catattccgg cacgccgttg ccgttgtgat cgcggttacg taaccaccag tcgtgatagg
    27301 ccaccagttt cgggtacatc tctgccaccc aggttttatc ttgggtgacg ttatacactt
    27361 ccatcaccga ccaggcggca aggctgggtt tggtattacg ttcgttccag ttgccgccat
    27421 cgccaccacg ctcggggcta aggttccacg ctatcaggtc ggggacaaag cccacatcct
    27481 gcggacgcac gcggtcgcct ggctggatct gccaggagaa gaccgcgcgg atattctctt
    27541 tggcgatgtc cggattgaaa tgcgccatcg caaacgcctg cttccaggta tcccacggcc
    27601 aggtctgatt gccggagaac cagcgcccgg tcaccgacgg tgtgacggtg ttaaatttca
    27661 ccgccccgcc aggcgaacgc cagttaccgt tgagcgtttc gatcgctttc accgcgacgc
    27721 gcgtctgttc cggcgtcgca tccggattgg ttaacccttt cttcagatac tcttcccagc
    27781 gttgctgcga ggcggtgaga taaaacgccg gacgcgccag aatatcgcgg atctgcattt
    27841 gctctttgct aacttcctga gaggtcagca gatgggaata ggtggtatag agcgtcgtcg
    27901 aaccgttgat atgtgcctta ctggtaaagc gattgccgtt gatttcagtc tgcaccggca
    27961 gggatttatg cacctgatat tctgattcac cggaggtcag cagatcccag gtggcgcgca
    28021 ctttgccaaa ggtgactttc aggccatcac gggtggcgct gattttgcgc tggtagtcag
    28081 ggtattcgcc agcaatggtt ttatcggaaa gcggtttccc ttctttcgct tccagttttt
    28141 ccagcagttc gccatcccac accagatcca gcggtttatt gctgatgatt ttagtttcca
    28201 gtagtgacgt gcgcggcgtg gcgaagcgca gagtcatttc gacctgcaca tcttttgctg
    28261 tcagtttttg caccagcgcg ccgggaatac tgtatgcctc cagcgtgaag tcgactttct
    28321 tgccgtcctg ccagacggtc aggtggtcga aattgctggc cataaagttg atgtactctt
    28381 ccgtcagcag cgcaacgcct ggaaaaccgc ccatggtgtt agggccgtct ggcaacagat
    28441 gaccatgcca ggctccgaga tcgaaaaacg gattaaaacg ctgatgatcg tcgtaatcgt
    28501 aatccttcat gtactgcggc gcaccagtac ggttaatcac gtttttataa ttgtcggcgt
    28561 tagcggcatg ggcggaaaaa cttatcagca gagcgcaggt tactggcgtt aaaatagttt
    28621 tgattttcat gaaattacag tccttattac cagtagaaat attgcatcag gaagatttgg
    28681 tcgctgggat cgtgagaggc atttttcatg tagaagcggc tatcaaaagc cgttgcgaaa
    28741 cgtccgccgc cgtattcgta ccagatacca aactgcatga aagaggtggt ttcatcgtca
    28801 gcgtggtcag ggcgatgttt ggcgtaggtg taagcggtgg agagataaac gccctttaat
    28861 gcttcataca tggcgctcac catcatccca ctttcacttc ccggtacgtc cagcccgtcg
    28921 gcacgtccgg tatggtgcca ggcgcgagcg gcaaagttag gggtaagcag gccggtgaga
    28981 tagagttgtc ctgtgccgtc ggtaatttcc agaccattca tccaggtgat gccttcggca
    29041 aggtcatact ggatgtaacc gttcatcatt gccggatagg tgtatttgtc gtcgtagcga
    29101 tcgtacttgc caaaatgtag ccaggctttg ctttcgtcat gatgtccggc aggtgtggcg
    29161 gtgacgctat aacgaaagtt gccgcttaag ttttgcacct tcagcgcagt cataatgtcg
    29221 cgggtattgg gaatgacgta gcccagatcg ggcgtgaaat cgccccacca ttgcaggtca
    29281 tccagcgagg aatcctgacg cagactgagc atgatttcgg tgccatcatt ggtacgataa
    29341 ccgccataga aacggttaat tccgccctcg aaaccacccc agctatggtc aggcacccag
    29401 gcattgccgt cgtggtcggc ctgcacggtc cagccttcgc cgtaaatcag gccgaaccag
    29461 gagccgtact tcatctccag gccaccttca atataggtgc cgtcactgta tttatgttta
    29521 ttctcaccct ctaaatccat atatccacca acccctaatt cgccgtaaaa acgaaatgaa
    29581 ggagaagatg tcgaggtaat ggcgggtgtt tttgtgtcgt catgttctgc cggacgtgct
    29641 tcttctcctg ccgcagaaaa ggcgtagcaa aacggcagag caagtaatgc tctgcatggt
    29701 gcagtgatta atttcattgt gttaatccat gagctaaaga aaaagtacag ccttttatcg
    29761 ttgtaattat aaaaatataa aggcagaaac tattttccct ctcctcatcc aggaagaggg
    29821 aaaatgcctg ctcatttttt attattctgc ttaatcaaca ttgctggctg gcgtcgcttc
    29881 aatatggcgc tcttcagccg ttaatccctt tatatattta ctgtatttcc accacgcaaa
    29941 gccaaggaag ataacaatac cgccgacgtt ataaaaaatg atggtcagaa tattcgcgcc
    30001 agtcgggaat gtcgaagcga caaaccctac ggcaaatatc gcaatcagca ttgaaacaac
    30061 gataataccg gtgcggcggg agcccatacg gaaatcgcgt ggcaggtgat ctaatttggc
    30121 gcgtaaattc aggtaagcca gcatgatgaa taacggcgga agcatggacg ctgcggcagt
    30181 catattaata atagtattca tcagatcctg cacggtattg gaaccgagca tcgggataat
    30241 catcagcggg atgacgatca ggaactggat ccacgctgcg cgcgccggaa cgccgttttc
    30301 gttcagttcg acggttttct taccaaagat gccttccggg atttcggaga agaaaatttt
    30361 caccggcgtt gcggtccaca tcagcaggga accgaacatc gcggtaaagg acaccagacc
    30421 gacaaagcga ttcatcaacg cttccggtag accaaaatac gccgccatgc cgtggaatac
    30481 ctgcaccgat ccgccggtaa atttcaactc tttgctgctg acgaagacgt taatcagcac
    30541 cgaggagacg gaatacagta cgccgataaa aatcccggcg aggatgatca ctttaacgaa
    30601 cgatttcgaa ccgcctttga cgtcgttaac gtacacagcg acggactccg cgccacctgc
    30661 ggccataaag atccaggtgg taacgccgag gaacgcccag ttgaagttcg ggatcatcgc
    30721 gtcaacggtg atggggtcag caggctgtac gccgccaacc agcgccgtac ctgccagtaa
    30781 aatgtaggag agcgttaaca gcagcatcag cgttgaagtg acggaggtaa ttggccccaa
    30841 cattttcgcc ccgttggtgg aaacccaggt ggagaaggcg aacagcacca tactgataat
    30901 ggtggtggca accggcgtca taatatattc gtagccgagg aaggcatacg aagcataagc
    30961 aataacgcgc ggcaacagtg aggtgaaaaa gaacaggttt acgaaccagt aggtataggc
    31021 agtaataaat gcccaacgtc cgcccagcga actttttacc cacgcgtaga caccggcttc
    31081 tgagttttta tttaacgaaa caaattctgc gatgatcaga cagaagggaa taaaataaaa
    31141 aatcgtcgcg aggaaaaaca tcggtgccga ggccagtcca agctcaatat tattattgat
    31201 aacgttgtta aagctgtaaa cggcggcaaa agtcagcgag agcaagccga atttgccgat
    31261 tgtattacgt ttggtatcag acataacaca tctccgaaaa tgtgtagggt agcggtggcg
    31321 gcaaactcct tttgccgccg tagttgttat ttgttatgga aataaccatc ttcgatggtg
    31381 actttgagaa ccactttttt gaccgcgtta ttgcagataa accgatacgc ttcatggata
    31441 tcgcaaataa cgatttgccc ttcgtggacc tcaacggttt ctccgcagcc ttttaaatat
    31501 tcacggtcag tttcatcacg ataatattcc actacctgta atgtctcttt cggcgcatat
    31561 tcaatttttt gctgcccttg caggtagtaa tgcacttcaa aatagcgtcg atgcccggta
    31621 aatagcgcat cggtcgcgga gtctgtctcc acgcggtaag tcaatgagtc accgatggag
    31681 tgggcgacgc caggctgaat gttgtcgata ttttcaatcg cttcaacgca gcgttgccac
    31741 ttcttgccag aggcgtaaat ctggcggaac tgttctaagt tatcgatgat cctcattgct
    31801 tattctcgct gtgcaaattc gtggaaaaga accctgcgcc gaactcatac gacgccaggc
    31861 tttgcgcggt agcttctccg ccagaaaccg gcagcaacgt aaagccgtag ctgaagtcac
    31921 ggaaccagac gcgccaggag tccagcacct cgctgcccca ggagttggag ccgaggccaa
    31981 gcagctggtg atcgaggttc agggtgatgt catcactgcg ctgcagctcg ttacagtgct
    32041 gggcagcgtg gatgttttcc tgggtatagt gccaggcgct gaagttaatt gggcgctgcg
    32101 gaaccaccag caaaccgttg ccgtggcggt tagtcagtgc cgtccagcgg acatgctgac
    32161 ggttgccgtt gttctgcggg aagggatagt tctcgaacat ggcatcgacg gtgctgcgcc
    32221 agatatcgat gatgttagcc tgctggctgt cggcgtagtt ttcgcccggt ccacgaccgt
    32281 aatacgccac ctgatcgtat tcgccgttaa tccccatggt gaaaccgatg cacggaatga
    32341 tgtgcggata gtcgccgtaa cgctcgccgg aaagcgccac gttaacctgg ccatcggcag
    32401 cgatgcgcca gatgtaggtg cagcgcatcc cgaagtcaaa caccggcggg gcaataactg
    32461 tgcggctgat gatcaacact tcaccatcgc tctgttctac ggcaaagtcg cgcagatgtt
    32521 cctgcatgat ctgcaaatga ttcggttgcc acagcccttc gtactcctgc ttgtggttgt
    32581 cgatcattgg cttgaagaag ttgatctttg gctcgcgggt cagcagcgac tcgccattca
    32641 cctgccagga tgtcggtttg ccactcattt ttgagaaggt gatcgcgaag ttgtagccgc
    32701 gaacggtgca gctcaaacga tcgtcttcca gcgtcagcgg acgcgcatta tttggtgcga
    32761 aaggcactgg ttgcgcggtg ttttccttca gcgggaactg ataagtggcg atagaatgcc
    32821 cggcttcgct gtagcgggtg cgggaatctt tggtcaccgt aatgttgagg aacgcttcgc
    32881 gggcgtccag ctgcggcagc gtgatctgca agggggcttc gctgttcggc gcaacgtcgc
    32941 gcagtttaat ctgctgcgtc gcgagcgttt caccttcggc gcgcacctct gcgtgcaggg
    33001 tgtagtcatc aagcgtggta aaccacagtt tattttcgac tttcagctcg ccgcgagtca
    33061 gatccagcgc gtggattttt accggcgcga taacctgttt gtactctttc aggcctggtc
    33121 ccggcgtctg atcggaatag atcaaaccat caagacagaa gttatagttg ttgggatagt
    33181 cgccgtagtc gccgccgaat ttataccaga cattgccgtt gtcatcctgc gcctggatcc
    33241 cgtggtcgca ccactcccaa acataatgtc cctgaataca atcgtgctta tagaagacgt
    33301 tctggtactc cgtcagcccg cccggtccgt tccccatcgc atgagcatat tcacagatga
    33361 tgcgcggctt cggatgcggg tattcaccaa actcattcat cagcggcacg cgggtgtaca
    33421 tggtggaaat aatatcgacc acttcagcat cgcgatcttc ttcgtaatgc accagtcgcg
    33481 tgtcatccag cgccttcgct gcgtggtaca tcgcgcggat gttacagcca tagccggatt
    33541 cattgcccag cgaccagatg atgatcgacg gatggttttt ctgcgcgtga atatggcgaa
    33601 caatgcgctc gacgtagact ttttcccact gcggatcgtc ggtaatacgg ctgatatcgc
    33661 cgacattagc aaagccgtgc gattcgacgt cggtttccgc catcacaaac aagccgtaga
    33721 tatcacacag ttcgtaaaaa cgcggatcgt tcgggtagtg agcggtacgc acggagttga
    33781 tgttgtgctg cttcatcaac tggagatctt tctcgacgcg atccattcca acggcgcggc
    33841 ctttgcgatg atcgttgtcg tgacggttga cgccgtgcag catcacataa cggttattga
    33901 tccagaacag accgtcgcgc actttgatat cacggaagcc aacgcgttgt ggtaccactt
    33961 ccagaacgtt gccgttggcg tctttcagcg tcatgaccag atggtaaaga taaggggatt
    34021 ctgctgacca ttgctgcggc tgttcgacag taaaagcaaa gctggcgctg gtcagttttt
    34081 caattgccaa atgatcaatg gcgctgctgt gcaccacgcg ttcgccatca aacagggtat
    34141 attccagcgt cgtgacgaca ggggaggcgg agagattttc cagcaccact tcgcaggaaa
    34201 gcgtggcatc gcaataggct tcgtcaaagt cggtacgaac ggtgaaatcg ttaatatgtg
    34261 ttagttgttt tccgatcaga taaacatcgc ggaagatccc cgctgaccac cacatatcct
    34321 ggtcttccac gtaggtagag tccgcccatt gcatcacgcg cacacacaac aggttgtcgc
    34381 cggttttaac catcgcgctg atgtcaaact ctgcggtcag gcgactgccc ttgctgaaac
    34441 ccacatactg accgttaacg tagacttcaa aataggtttc gacgccgtca aatttaatca
    34501 gcgtctgttt accctgccag ccgtcgctga gggtgaaaat acgttgatag gcaccggttg
    34561 ggttatcgct ggggacaaac ggcacatcga tggggaacgg aaaaccttcg tcggtatatt
    34621 gcagtttgcc gtgaccttcc atttgccaca tggcggggac ggtaatatgc ccccagtcag
    34681 ccattaactc agaggtgaag gcttcaggca cttgcagcgg atggtcaaaa aagtggaaat
    34741 tccactggcc gcttaagggc agaaacaggc tgctggtttc gcgggcaaag gtacgcgctt
    34801 gcgcaacaga atcatatgaa aaaaagtacg cacgtggcgc aagtcggttt tcgtgggtga
    34861 gctgaatgtt ttcccagcga ttcatagggc ctcccggtag agagaaatcg gaaccattca
    34921 tggtgatggt agtttgtgta attgctgggg agtttagtaa aatttttact aaaaggtagc
    34981 gggtaaatgc atttacttta actggatcac gaaaataaac ggcagcactg tccggcaaaa
    35041 acgattgccg gacagcgtgc aaggggattt aacgggtcgt gccgcgcagt tttaatttgc
    35101 tgggaacgaa gaccaacagc ggcagcgcgc gaccatcgcg ggctttttca tacaccaggt
    35161 taacgccctg acttcccatc atctcggaat ggatgcgcac ggtggagagc ggcggaaagg
    35221 taaatcgcgc ggtggggata tcgttaacgc tgataagcga aatatcctgt gggatgttca
    35281 ggccacgttc atgaattgcc cgcagtacgc cgatagcaat ggaatcagaa gcaacaaaca
    35341 gtgccttcgg atagtcttcc tgcgccagca gctgttttgc cagttcatag cccgacgaac
    35401 tggaaaagcc tccgcgccag atatcctctt cgcgtaccac ttgtttcagt cggccatatt
    35461 ccgcaaaggc gacctcacga atatccgctt tgcctggctc atcttcaccg ccaataaaac
    35521 caatacgatt aacgccctga ttgatataga agtcgatgat ttctttactg atgcgtgcca
    35581 gatcgatatc caccgcatcg taaccgctgc cgggttcgtg aaagtcgata aaacagatat
    35641 tgtcggtcaa cgcacaggcg gcggcgcgca gggcgggcgt gggtttgccg acaattaaaa
    35701 taccggtgac gtttttaatg tctggtaatc cgctgtgttc ataacagttg gtgagctcaa
    35761 tgcccagctt ttcgcactga gtttcaatgc cgtggcggat cgccagatag taaggatcgt
    35821 tgatctccag ctcctgttgg tagctgtaga tagccagaat atggtgttgg ttgactgcgc
    35881 ctgtctggag tttacgggca ctactggtct tgtactccag cttttcggcg atctcgagaa
    35941 tgcgatgttt cgtctcttct ttcacattca atgtcggatc gtcatttaag accctggata
    36001 ctgtcgccag ggatacgcca gcttcgattg cgatgtcttt tagtgttgcc atgtttacct
    36061 tttttacttt ccctgatgtg cgcccccatt gtagaggaag tgcttatcgg gtcacctgtt
    36121 ttcagtaaat aaagcgcgtt tctttgacgc tgtaacggta acacagcggc tcacgaatgt
    36181 gatcacgcct gcattctcca tttttagcct acaggattga gaaaaacttt tgctatagtt
    36241 tagtaaaatt ttacttgaag ggaggaagca atggaaaccg tggcttacgc tgattttgca
    36301 cgtctggaaa tgcgcgtcgg aaagattgtg gaagtgaaac gccatgaaaa cgccgacaag
    36361 ctgtacatcg tacaggttga tgtgggggaa aaaacactgc aaaccgtgac cagcctggtg
    36421 ccgtactaca gcgaagaaga gctgatgggg aaaacggtgg tggtcttatg caatctgcaa
    36481 aaagcgaaaa tgcgtggtga aacgtcggaa tgcatgctgt tgtgtgcgga aaccgatgac
    36541 ggcagtgaaa gcgtgttgtt aacgccggag cggatgatgc ctgcgggcgt acgcgtcgta
    36601 taaaaagatc ggatggcgac gtcgtatcgc catccgattt gatattacgc ttcttcgaca
    36661 cttactcgca tggccgccag cgccttacgc gccgctttga tcaccagttc acactgttca
    36721 atggtcagtg tcagtggcgg ttcaatgcgg atcgttttgg cgttattgag cgttccggcc
    36781 accagtacgc gctggcggaa catctcgctg gcaaagttat agccgatttc gttatcaaca
    36841 aactcaattg ccatcaacat ccctttacca cgcgcttcct gtaccagatc gggatattcc
    36901 cgcgccagtt gacggaaacc gtccagcaac atatcgcctt tttgctcagc ctgagccggt
    36961 aagttctgct ccagcaacac attgatggtc gccagcgccg ccgcacaggc cagcgggttg
    37021 ccgccaaagg tggtggtatg caggaatggg ttgtcgaaca gaactgaaaa cacctcttca
    37081 gtggcgatgg tcgcgccaat cggcatcacg ccgccgccga gcgctttggc aaggcagagg
    37141 atatccggct gtacgttctc atgctcgcag gcgaacatct tgcccgtgcg ccccatgccc
    37201 gtttgtactt catcgaggat catcagtgcg ccgaactcat cgcatagctt acgtacggcg
    37261 gtgagatagc ccggcggcgg cagaattacg ccaccttcac cctgaatcgg ttcgaggatc
    37321 accgcagcca catcatcacc ggtttttttg cactcgttaa gagccgtgcg catggcttcg
    37381 atattgccaa acggcacatg acggaagccc ggcagtaacg gcataaacgg tttgcggaag
    37441 gtcgatttcg ccgtggccga cagcgcgcca agtgatttac cgtggaacgc gccgctggtg
    37501 gcaataaaag taaacttgcc gcgcggtgac tggtaagctt tcgccagctt cagcgctgct
    37561 tcaacggact cggtgccgct attacagaag aagctgtatt tcagtttacc gggcgttagc
    37621 gcagcaaggg ttttcgccaa catcgcccgt aacggatcga gcagctcctg gctgtgcagc
    37681 ggttgtttcg caagttgatt ctgtacggcg gaaaccacaa ctggattacg gtgccccacg
    37741 ttgaaaattc caaaacctcc caggcagtcg ataaactcct gtccctgggt gtcgacaagc
    37801 gtatttaaac ctcccgcttg ccactctacg gctccgtaat ccccgccggc ggtaacagat
    37861 ttgcgatact ctaaaaaccc cggattgaca tgctctttga agtattcaat cacctctcgg
    37921 ttaagtgctt tcatctcctc atgatccagc gttcgcttct caatgagatt cagggcgtgg
    37981 gcgctgcacg ctaaagccga tgcgctcgaa ggtaacctgt tcaaaatatg ctccggaggc
    38041 tcgcgtatca catgataccg atttaagtat tgcagggatt gcgccactcc ggctgcgatc
    38101 gcgaaaatcg ggataaacac aaatgtggtt atcatttgcg caatatttaa tgctatttgt
    38161 taacatctgt tgttatttcg cacggttttt gtcgggtatg gatttgatga ttgctgcgca
    38221 aatgcacgtt ttcagggcaa taattgcctg atttttgggc aagataatta acgttttcgt
    38281 atagtaacag agtgaaagta ttttaaatca aagaattaga tttgtgcttt aatcgcaaat
    38341 tgcgatctaa atcaaattaa tcggttaaag ataaccgcag cggggccgac ataaactctg
    38401 acaagaagtt aacaaccata taacctgcac aggacgcgaa catgtcttct catccgtatg
    38461 tcacccagca aaataccccg ctggcgggcg ataccactct gatgtccact accgatctgc
    38521 aaagctatat cactcatgct aatgacactt ttgtgcaggt gagcggcttt accttgcaag
    38581 agttacaagg gcagccgcac aacatggtgc gtcacccgga tatgccaaaa gcggcgtttg
    38641 cggatatgtg gttcaccctg aaaaaagggg agccctggag cggcatcgtg aaaaatcgcc
    38701 gcaaaaatgg tgaccattat tgggtgcggg ccaatgcggt accgatggtg cgcgagggaa
    38761 aaatcagtgg ctatatgtcg attcgtaccc gggcgacgga tgaagagatc gcggcggtgg
    38821 agccgctgta caaagcgctg aacgccggac gtaccggtaa gcgtattcat aaaggcctgg
    38881 tggtgcgtaa aggctggctg ggtaaactgc cttcattacc gcttcgctgg cgggcgcgtg
    38941 gagtgatgac cctgatgttt atcttgctgg cggccatgct ttggtttgtt gctgccccgg
    39001 tggtgacgta tatcctctgt gcgttagtga tgttgttggc aagcgcctgt tttgaatggc
    39061 agattgtccg cccgatagaa aatgtcgccc gtcaggcact gaaggtggcg accggagagc
    39121 gtaatagtgt tgagcatctg aatcgcagcg atgagctggg gctgacatta cgcgcggttg
    39181 ggcagcttgg cctgatgtgc cgttggctaa ttaacgatgt ctcaagccag gtgtccagtg
    39241 tcagaaatgg cagtgagacg ctggcgaaag gcaccgatga actgaacgaa catacccagc
    39301 agacagttga taacgttcag caaacggtgg cgaccatgaa ccaaatggcg gcgtcggtga
    39361 aacagaactc tgccacggcg tcggctgccg ataaactttc tatcaccgcc agtaatgcgg
    39421 cagtgcaggg cggggaggcg atgactacgg tgatcaagac aatggacgat attgccgaca
    39481 gtacccagcg cattggcacc attacttcgc tgattaacga tattgcgttt cagaccaata
    39541 ttctggccct gaatgcggcg gtggaagcgg cgcgtgccgg cgaacagggc aaaggttttg
    39601 cagtggtggc gggggaagtg cgtcatttag ccagccgcag cgctaatgct gccaacgata
    39661 ttcgcaagct gattgatgcc agtgctgata aggtgcaatc cggttcgcag caggtacatg
    39721 ccgccggacg gacgatggaa gatattgtgg cacaggtgaa aaacgtcacc cagttgatcg
    39781 cccagattag ccattcaacg ctggaacagg ccgatgggct ttccagcctg acccgtgcag
    39841 tggatgagct taacctgatc acccagaaaa atgccgagct ggtggaagag agtgcgcagg
    39901 tgtcggcgat ggtgaaacac cgcgccagcc gactggaaga cgcggtgacg gtgctgcatt
    39961 aatcggtacg ccggatccgg ctgttggagc acaatgcctg atgcgatgct ggcgcatctt
    40021 atcaggccta cggggtgtgt agcgggtgta ggccggataa ggcgcgtcaa gcgtcgcatc
    40081 cggcaattgc accgcgccac tggcggatgc ggcgattaat ccagttgcgt aatatcaaaa
    40141 gcggcgcggt cgatcaccgc aatgatcttt tttatttgtg catcagtgat atcgctctgg
    40201 ttgacgcgta aatccagcac cgctttaaaa ttatccagcg cccgcttcat ttgcgggttc
    40261 tggcgcagcg ccgcgccaac gcaacgcgct ttgatgcgtt cttcaatcat ctccacctgt
    40321 tcgcggtttt cttccagcca ctgcgcgcct tgttcggtca gtgcaatctg cttcttacca
    40381 ccttcctctt cgcggatggt aatcagcgac tgctcctgca gaaaatccag cgtcgggtag
    40441 atgacgcccg ggcttggggt gtaattcccc tgggttagat tctcaatcgc tttaatcaat
    40501 tcgtaaccgt ggctgtcatc gcgcgagaga atatccagaa tcaccagacg caattcaccg
    40561 tgaccaaaga aacgttgccg acgaccgccg ccgcgaccgt ggcgaccacc gcagcattga
    40621 ccgtgttcac gctggtgacc gtgtccgcag tgctcgtgtt ctgacttctc acctttgcag
    40681 cagccctcat ggcgtggctg gccttcatgt
//
LOCUS       DAJOWB010000029        40490 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJOWB010000029 DAJOWB010000000
VERSION     DAJOWB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.029"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            290..376
                     /gene="leuL"
                     /locus_tag="OHO85_001725"
     CDS             290..376
                     /gene="leuL"
                     /locus_tag="OHO85_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCQ2671107.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            469..2040
                     /gene="leuA"
                     /locus_tag="OHO85_001726"
     CDS             469..2040
                     /gene="leuA"
                     /locus_tag="OHO85_001726"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCQ2671108.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2040..3131
                     /gene="leuB"
                     /locus_tag="OHO85_001727"
     CDS             2040..3131
                     /gene="leuB"
                     /locus_tag="OHO85_001727"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCQ2671109.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3134..4534
                     /gene="leuC"
                     /locus_tag="OHO85_001728"
     CDS             3134..4534
                     /gene="leuC"
                     /locus_tag="OHO85_001728"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCQ2671110.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4545..5150
                     /gene="leuD"
                     /locus_tag="OHO85_001729"
     CDS             4545..5150
                     /gene="leuD"
                     /locus_tag="OHO85_001729"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCQ2671111.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5199..6377)
                     /gene="setA"
                     /locus_tag="OHO85_001730"
     CDS             complement(5199..6377)
                     /gene="setA"
                     /locus_tag="OHO85_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCQ2671112.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6479..6610)
                     /gene="sgrT"
                     /locus_tag="OHO85_001731"
     CDS             complement(6479..6610)
                     /gene="sgrT"
                     /locus_tag="OHO85_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCQ2671113.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6699..8354
                     /gene="sgrR"
                     /locus_tag="OHO85_001732"
     CDS             6699..8354
                     /gene="sgrR"
                     /locus_tag="OHO85_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCQ2671114.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8388..8487
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8518..9501
                     /gene="thiB"
                     /locus_tag="OHO85_001733"
     CDS             8518..9501
                     /gene="thiB"
                     /locus_tag="OHO85_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCQ2671115.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9477..11087
                     /gene="thiP"
                     /locus_tag="OHO85_001734"
     CDS             9477..11087
                     /gene="thiP"
                     /locus_tag="OHO85_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCQ2671116.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11071..11769
                     /gene="thiQ"
                     /locus_tag="OHO85_001735"
     CDS             11071..11769
                     /gene="thiQ"
                     /locus_tag="OHO85_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCQ2671117.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11883..12647)
                     /locus_tag="OHO85_001736"
     CDS             complement(11883..12647)
                     /locus_tag="OHO85_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCQ2671118.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12755..13612)
                     /gene="araC"
                     /locus_tag="OHO85_001737"
     CDS             complement(12755..13612)
                     /gene="araC"
                     /locus_tag="OHO85_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCQ2671119.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKGE"
     gene            13951..15651
                     /gene="araB"
                     /locus_tag="OHO85_001738"
     CDS             13951..15651
                     /gene="araB"
                     /locus_tag="OHO85_001738"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCQ2671120.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15662..17164
                     /gene="araA"
                     /locus_tag="OHO85_001739"
     CDS             15662..17164
                     /gene="araA"
                     /locus_tag="OHO85_001739"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCQ2671121.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17364..18059
                     /gene="araD"
                     /locus_tag="OHO85_001740"
     CDS             17364..18059
                     /gene="araD"
                     /locus_tag="OHO85_001740"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCQ2671122.1"
                     /translation="MLEDLKRLVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIATGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18134..20485
                     /gene="polB"
                     /locus_tag="OHO85_001741"
     CDS             18134..20485
                     /gene="polB"
                     /locus_tag="OHO85_001741"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCQ2671123.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDIRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYIRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20650..23556
                     /gene="rapA"
                     /locus_tag="OHO85_001742"
     CDS             20650..23556
                     /gene="rapA"
                     /locus_tag="OHO85_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCQ2671124.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23568..24227
                     /gene="rluA"
                     /locus_tag="OHO85_001743"
     CDS             23568..24227
                     /gene="rluA"
                     /locus_tag="OHO85_001743"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCQ2671125.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24344..25159)
                     /gene="djlA"
                     /locus_tag="OHO85_001744"
     CDS             complement(24344..25159)
                     /gene="djlA"
                     /locus_tag="OHO85_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCQ2671126.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQTGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25414..27768
                     /gene="lptD"
                     /locus_tag="OHO85_001745"
     CDS             25414..27768
                     /gene="lptD"
                     /locus_tag="OHO85_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCQ2671127.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNSL"
     gene            27821..29107
                     /gene="surA"
                     /locus_tag="OHO85_001746"
     CDS             27821..29107
                     /gene="surA"
                     /locus_tag="OHO85_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCQ2671128.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATADIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29107..30096
                     /gene="pdxA"
                     /locus_tag="OHO85_001747"
     CDS             29107..30096
                     /gene="pdxA"
                     /locus_tag="OHO85_001747"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCQ2671129.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRTYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPLLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30093..30914
                     /gene="rsmA"
                     /locus_tag="OHO85_001748"
     CDS             30093..30914
                     /gene="rsmA"
                     /locus_tag="OHO85_001748"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCQ2671130.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30917..31294
                     /gene="apaG"
                     /locus_tag="OHO85_001749"
     CDS             30917..31294
                     /gene="apaG"
                     /locus_tag="OHO85_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCQ2671131.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31301..32143
                     /gene="apaH"
                     /locus_tag="OHO85_001750"
     CDS             31301..32143
                     /gene="apaH"
                     /locus_tag="OHO85_001750"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCQ2671132.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32221..32700)
                     /gene="folA"
                     /locus_tag="OHO85_001751"
     CDS             complement(32221..32700)
                     /gene="folA"
                     /locus_tag="OHO85_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCQ2671133.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32892..34754)
                     /gene="kefC"
                     /locus_tag="OHO85_001752"
     CDS             complement(32892..34754)
                     /gene="kefC"
                     /locus_tag="OHO85_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCQ2671134.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTMMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34747..35277)
                     /gene="kefF"
                     /locus_tag="OHO85_001753"
     CDS             complement(34747..35277)
                     /gene="kefF"
                     /locus_tag="OHO85_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCQ2671135.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35385..36716)
                     /locus_tag="OHO85_001754"
     CDS             complement(35385..36716)
                     /locus_tag="OHO85_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2671136.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(36774..37061)
                     /gene="fixX"
                     /locus_tag="OHO85_001755"
     CDS             complement(36774..37061)
                     /gene="fixX"
                     /locus_tag="OHO85_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCQ2671137.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37058..38344)
                     /locus_tag="OHO85_001756"
     CDS             complement(37058..38344)
                     /locus_tag="OHO85_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCQ2671138.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38395..39336)
                     /locus_tag="OHO85_001757"
     CDS             complement(38395..39336)
                     /locus_tag="OHO85_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCQ2671139.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(39351..40121)
                     /locus_tag="OHO85_001758"
     CDS             complement(39351..40121)
                     /locus_tag="OHO85_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCQ2671140.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         9752 a        11136 c        10635 g         8967 t
ORIGIN      
        1 catataattc aatacaaatc atcaggatag gttttgcaac gcgtgcattt tgtccccttt
       61 ttcctcgttg attagatgca aaaatttatg ctgaaatatg tcaaccgatg aaaagcgtcg
      121 gtagttaagc agaaattaat atcgcttact ttaaccaccg cagcacaatt agctaatttt
      181 acgggtgcag aactcacgct ggcgggacgt ttttattgcg tcagggttga catccgtttt
      241 tgtatccagt aactctaaaa gcatatcgca ttcatctgga gctgatttaa tgactcacat
      301 cgttcgcttt atcggtctac tactactaaa cgcatcttct ttgcgcggta gacgagtgag
      361 cggcatccag cattaagcca gcacgcagtc aaacaaaaaa cccgcgccat tgcgcgggtt
      421 tttttatgcc cgaagcgagg cgctctaaaa gagacaagga cccaaaccat gagccagcaa
      481 gtcattattt tcgataccac attgcgcgac ggtgaacagg cgttacaggc aagcttgagt
      541 gtgaaagaaa aactgcaaat tgcgctggcc cttgagcgta tgggtgttga cgtgatggaa
      601 gtcggtttcc ccgtctcttc gccgggcgat tttgaatcgg tgcaaaccat cgcccgccag
      661 gttaaaaaca gccgcgtatg tgcgttagct cgctgcgtgg aaaaagatat cgacgtggcg
      721 gccgaatccc tgaaagtcgc cgaagccttc cgtattcata cctttattgc tacctcccca
      781 atgcacatcg ccaccaagct gcgcagcacc ctggacgagg tgatcgaacg cgctatctat
      841 atggtgaaac gcgcccgtaa ttacaccgat gatgttgagt tttcttgcga agatgccggg
      901 cgtacaccca ttgccgatct ggcgcgagtg gtcgaagcgg cgattaatgc cggtgccacc
      961 accatcaaca ttccggacac cgtgggctac accatgccgt ttgagttcgc cggaatcatc
     1021 agcggcctgt atgaacgcgt acctaacatc gacaaagcca ttatttccgt acatacccac
     1081 gacgatttag gcctggcggt cggcaactca ctggcggcgg tacatgccgg tgcacgccag
     1141 gtggaaggtg caatgaacgg gatcggcgag cgtgccggta actgttcgct ggaagaagtc
     1201 atcatggcga ttaaagttcg taaggatatt ctcaacgtcc acaccgccat taatcaccag
     1261 gagatatggc gcaccagcca gttagttagc cagatttgta atatgccgat cccggcaaac
     1321 aaagccattg ttggcagcgg cgcattcgct cactcctccg gtatccacca ggatggcgtg
     1381 ctgaaaaacc gcgaaaacta cgaaatcatg acaccagaat ctattggtct gaaccaaatc
     1441 cagctgaatc tgacctctcg ttcggggcgt gcggcggtga aacatcgcat ggatgagatg
     1501 gggtataaag aaagtgaata taatttagac aatttgtacg atgctttcct gaagctggcg
     1561 gacaaaaaag gtcaggtgtt tgattacgat ctggaggcgc tggccttcat cggtaagcag
     1621 caagaagagc cggagcattt ccgtctggat tacttcagcg tgcagtctgg ctctaacgat
     1681 atcgccaccg ccgccgtcaa actggcctgt ggcgaagaag tcaaagcaga agccgccaac
     1741 ggtaacggtc cggtcgatgc cgtctatcag gcaattaacc gcatcactga ctataacgtc
     1801 gaactggtga aatacagcct gaccgccaaa ggtcacggta aagatgcgct gggtcaggtg
     1861 gatattgtcg ccaactacaa cggtcgccgc ttccacggcg tcggcctggc caccgatatt
     1921 gtcgagtcct ccgccaaagc catggtgcac gtactgaaca atatctggcg tgccgcagaa
     1981 gtcgaaaaag agttgcaacg caaagctcaa cacaacgaaa acaacaagga aaccgtgtga
     2041 tgtcgaagaa ttaccatatt gccgtattgc cgggggacgg tattggtccg gaagtgatga
     2101 cccaggcgct gaaagtgctg gatgccgtgc gcaaccgctt tgcgatgcgc atcaccacca
     2161 gccattacga tgtaggcggc gcagccattg ataaccacgg gcaaccactg ccgcctgcga
     2221 cggttgaagg ttgtgagcaa gccgatgccg tgctgtttgg ctcggtaggc ggtccgaaat
     2281 gggaacattt accaccagac cagcaaccag aacgcggcgc gctgttgcct ttgcgtaagc
     2341 acttcaaatt attcagcaac ctgcgcccgg caaaactgta tcaggggctg gaagcattct
     2401 gtccgctgcg tgcagacatt gccgctaacg gcttcgacat cctgtgcgtg cgcgaactga
     2461 ccggcggcat ctatttcggt cagccaaaag gccgcgaagg tagcggacaa tatgaaaaag
     2521 cgtttgatac cgaggtgtat caccgttttg agatcgagcg tattgcccgc atcgcgtttg
     2581 aatctgctcg taaacgtcgc cacaaagtca cctcaatcga caaagctaac gtgctgcaat
     2641 cctctatttt atggcgtgaa atcgtcaacg agatcgccac ggaatacccg gatgtcgaac
     2701 tggcgcatat gtacatcgac aacgccacca tgcagctgat taaagatcca tcacagtttg
     2761 acgttctgct gtgctccaac ctgtttggcg acattctgtc tgacgagtgc gcaatgatca
     2821 ctggctcgat ggggatgttg ccttccgcca gcctgaacga gcaaggtttt ggtctgtatg
     2881 aaccggcagg cggctcagca ccagatatcg caggcaaaaa catcgccaac ccgattgcgc
     2941 aaattctatc gctggcactg ctgctgcgct acagcctgga tgccgatgat gcggcttgcg
     3001 ccattgaacg cgccattaac cgcgcattag aagaaggcat tcgcaccggg gatttagccc
     3061 gtggcgctgc cgccgttagt accgatgaaa tgggcgatat cattgcccgc tatgtggcag
     3121 aaggggtgta atcatggcta aaacgttata cgaaaaatta ttcgacgctc acgtagtgta
     3181 cgaagccgaa aacgaaaccc cgctgttata tatcgaccgc cacctggtgc atgaagtgac
     3241 ctcaccgcag gcgttcgatg gtctgcgcgc ccacggtcgc ccggtacgtc agccgggcaa
     3301 aaccttcgcc actatggatc acaacgtctc tacccagacg aaagacatta atgcctgtgg
     3361 tgaaatggcg cgcatccaga tgcaggagct gattaaaaac tgcaaagaat ttggcgtcga
     3421 actgtatgac ctgaatcacc cgtatcaggg gatcgtccac gtaatggggc cggaacaggg
     3481 cgtcaccttg ccggggatga ccattgtctg cggcgactcg cataccgcca cccacggcgc
     3541 gtttggcgca ctggcctttg gtatcggcac ttccgaagtt gaacacgtac tggcaacgca
     3601 aaccctgaaa cagggccgcg caaaaaccat gaaaattgaa gtccagggca aagccgcgcc
     3661 gggcattacc gcaaaagata tcgtgctggc aattatcggt aaaaccggta gcgcaggcgg
     3721 caccgggcat gtggtggagt tttgcggcga agcaatccgt gatttaagca tggaaggtcg
     3781 tatgaccctg tgcaatatgg caatcgaaat gggcgcaaaa gccggtctgg ttgcaccgga
     3841 cgaaaccacc tttaactatg tcaaaggccg tttgcatgcg ccgaaaggca aagatttcga
     3901 cgacgccgtt gcctactgga aaaccctgca aaccgacgaa ggcgcaactt tcgataccgt
     3961 tgtcactctg caagcagaag aaatttcacc gcaggtcacc tggggcacca atccaggcca
     4021 ggtgatttcc gtgaacgaca atattcccga tccggcttcg tttgccgatc cggttgaacg
     4081 cgcgtcggca gaaaaagcgc tggcctatat ggggctgaaa ccgggcattc cgctgaccga
     4141 agtggctatc gacaaagtgt ttatcggttc ctgtaccaac tcgcgcattg aagatttacg
     4201 cgcggcagcg gagatcgcca aagggcgaaa agtcgcgcca ggcgtgcagg cactggtggt
     4261 tcccggctct ggcccggtaa aagcccaggc ggaagcggaa ggtctggata aaatctttat
     4321 tgaagccggt tttgaatggc gcttgcctgg ctgctcaatg tgtctggcga tgaacaacga
     4381 ccgtctgaat ccgggcgaac gttgtgcatc caccagcaac cgtaactttg aaggccgcca
     4441 ggggcgcggc gggcgcacgc atctggtcag cccggcaatg gctgccgctg ctgctgtgac
     4501 cggacatttc gccgacattc gcaacattaa ataaggagca caccatggca gagaaattta
     4561 tcaaacacac aggcctggtg gttccgctgg atgccgccaa tgtcgatacc gatgcaatca
     4621 tcccgaaaca gtttttgcag aaagtgaccc gtacgggttt tggcgcgcat ctgtttaacg
     4681 actggcgttt tctcgatgaa aaaggccaac agccaaaccc ggacttcgtg ctgaacttcc
     4741 cgcagtatca gggcgcttcc attttgctgg cacgagaaaa cttcggctgt ggctcctcgc
     4801 gtgagcacgc accgtgggca ttgaccgact acggttttaa agtggtgatt gcgccgagtt
     4861 ttgctgacat cttctacggc aatagcttta acaaccagct gctgccggtg aaattaagcg
     4921 atgcagaagt ggacgaactg tttgcgctgg tgaaagccaa tccggggatc catttcgacg
     4981 tggatctgga agcgcaagag gtgaaagcgg gagagaaaac ctatcgcttt accatcgatg
     5041 ccttccgccg ccactgcatg atgaacggtc tggacagtat tgggcttacc ttgcagcacg
     5101 acgacgccat tgccgcttat gaagcaaaac aacctgcgtt tatgaattaa tccccttgcc
     5161 cggtcaaatg accgggcttt ccgctatcgt ccacgtcatc aaacgtcttt aacctttgcg
     5221 gttaaaaata atgcgacaac agaaataacc gcaattaccc agtagacagc aaagtgcccc
     5281 caactttgtg caattgctcc ctgaataacg ccagccagaa ttaccccggt agaaatactg
     5341 ttagtaaata aggtggtagc tgcccccgct cttccaggca ttaaatcctg aaaccatagc
     5401 atcccaatac ccgcaacaat gccgataaat acagcgttaa aaagttgcag cgtcatcaac
     5461 gccatacggc tatgaaagaa aatcaatccg gtgtaaaaca gtactcctgc cgccactgct
     5521 atgaccatca ttcgccgctt accataacgt ttgacatagt agccagccag aatcattgct
     5581 ggtatttcca gtccagctgc cgtccccatc aggaaacccg ccagtttgtc tggcaatcct
     5641 aactcgctac tgatccacaa cggcatatca ataatgtaca tggtgttgca ggtccacatt
     5701 aacgtcgagg cgacaaataa catccgtacg ttactatcct gccagccacc ttgcattgat
     5761 aaagcatttt ccgacggcag ttctacccgc gccacagacg gaagcataaa tgcaatcaat
     5821 accagactga gtgtgaatat cccggcggca atcgaaaaca tcaccgtaaa gccgtaattc
     5881 aacgccagca taaaggccaa cggtggaccg ataacccatg ccagagaaag ctgcgcacgc
     5941 atcaccgagc taaacatcac cacttctcgc gccgagttat ccgcatattc ccgcgccaga
     6001 gcaaataact gtggcattgc cgtattggcc agagatgcca gaagcacacc acaggtgata
     6061 agcgtcagat aatgacgatt aaatgcaaac aatagcgcat tgccgatagc catcaaacag
     6121 caaaatataa tcagttttcg ccgatcgccc tgactgtcag aacgttttgc caaccagagg
     6181 cttacgccga tcccagcaat agcattcacc gtataaaaga gtccgatcca gaaaggttgc
     6241 gcgccaacct cacgactcag aaataagctc aatgtaggag cctgtagcgc cccggccacc
     6301 cccatcataa aagcgaccag cataaatgcc gcgtaaacac cgttcatacg gcgagccatc
     6361 gtcattatcc agatcatacg ttcccttttt agcgcggcga gaataaaaaa aaccagcagg
     6421 tataatctgc tggcgggtga ttttacacca atactcagtc acacatgatg caggcaagtc
     6481 aactttcaga attgcggtca tcccactgca tcagtccttc aagcatggtt aatcgttgtg
     6541 ggacgcttaa ccaacgcaac cagcacaact tcgctgtcgc ggtaaaatag tgctgataaa
     6601 actgacgcat ggggcacctc cttgcttcat cgttaggaat tattgactta atatagggaa
     6661 aataaaattg ctgtcttttg cgcaggagtt ccccttttat gccatctgct cgtctgcaac
     6721 aacagttcat ccgcctgtgg caatgctgcg agggtaaatc gcaggacaca acgctcaacg
     6781 aactggcagc gttattgagc tgctcgcgtc gtcatatgcg taccctgctc aacaccatgc
     6841 aggatcgcgg ctggctgacg tgggaagcgg aagtcgggcg cggtaaacgc tcgcgtctga
     6901 cattcctcta taccgggctg gcgcttcagc aacagcgggc ggaagacctg ctggagcagg
     6961 atcgtatcga ccaactagtg cagttggttg gcgacaaagc gactgtgcgg caaatgctgg
     7021 tttctcatct gggccgcagc ttccgccagg ggcggcacat cctgcgcgtg ctctactatc
     7081 gtccgttgcg taatctgcta cctggcagcg cattgcgccg ttccgaaacc catattgccc
     7141 ggcaaatctt cagttcgcta acgcgcataa atgaggaaaa tggggaactg gaagcagaca
     7201 tcgcccacca ctggcagcaa atatcaccgc ttcactggcg tttctttttg cgtccaggag
     7261 tccattttca tcatggtcgt gaactggaaa tggacgacgt gatcgcctct ttaaaacgaa
     7321 tcaatacgct gccgctctat tcgcatattg cggacattgt gtcgccgacg ccctggacgc
     7381 tggatatcca tctcacgcaa ccggaccgct ggttaccgtt actgctggga caagttccgg
     7441 cgatgatcct gccgcgcgaa tgggaaaccc tcagtaactt tgccagccat cccatcggca
     7501 ccggtccgta tgcggtgatc cgcaacacca ccaatcaact gaaaattcag gcattcgatg
     7561 acttcttcgg ttaccgggca ttaatcgatg aagttaacgt ctgggttctg ccggaaattg
     7621 ccgacgagcc agctggaggt ctgatgctaa aaggtccaca gggcgaggaa aaagagattg
     7681 aaagccgcct ggaggaaggt tgctactatt tactgttcga cagccgcacc catcgcgggg
     7741 cgaatcagca agtcagggac tgggtaagct atgtgctttc tccaactaat ctggtctatt
     7801 tcgctgagga acagtaccag caactgtggt tcccggctta tggactgctc ccccgttggc
     7861 accatgcccg caccataaag agcgaaaaac cggctggcct ggaaagcctc accctgacct
     7921 tttatcagga tcacagtgag catcgggtga ttgccgggat catgcagcag attctggcaa
     7981 gtcaccaggt cacgctggaa atcaaagaga tcagctacga tcagtggcat gaaggagaga
     8041 tcgagagcga tatctggctt aatagcgcca actttacgct gccgctggat ttttcgctgt
     8101 tcgcgcactt gtgcgaggtg ccgctgctcc aacactgtat tccgatcgac tggcaagccg
     8161 acgccgcccg ctggcgcaat ggcgaaatga acctggcgaa ctggtgccag caactggtcg
     8221 ccagtaaagc aatggtgccg cttatccacc actggctgat cattcaggga caacgcagta
     8281 tgcgcggcct gcgtatgaat accctcggct ggttcgattt taaatcagcg tggtttgcgc
     8341 cgccggatcc atgattgctg gtagataaca aaatcactac actaacgccg ttctcaacgg
     8401 ggtgccacgc aaacgcgtgc gctgagaaaa tacccgtcga acctgatccg gataacgccg
     8461 gcgaagggat ttgaggctcc ttctcaagtc ctttgccact cttttttgag gtgcaaagtg
     8521 ttaaaaaaat gtcttcccct gctgttgctg tgcacagcgc ccgttttcgc taaacccgtt
     8581 ctgactgttt atacctacga ttccttcgcc gccgactggg ggcctggtcc aaagatcaaa
     8641 aaagcttttg aagccgactg taactgcgaa ctgaaactgg tggcgctgga agatggcgtt
     8701 tcgcttctca accgtctacg gatggaaggc aaaaatagta aagccgatgt ggtgctgggg
     8761 ctggataaca atctgttaga cgccgccagt aaaaccggac tatttgccaa aagcggtgtg
     8821 gcagcggatg ccgttaacgt tcccggcggc tggaataatg acactttcgt cccgtttgat
     8881 tacggctatt tcgccttcgt ctatgacaag aacaaactga aaaacccgcc gcaaagcctg
     8941 aaagaattgg ttgagagcga tcaaaactgg cgggtgattt atcaggatcc gcgcaccagt
     9001 acgccggggc tgggcctgct gttgtggatg caaaaagtct atggcgatga cgccccacaa
     9061 gcctggcaaa aactggcgaa gaaaaccgtc acggtcacca aaggctggag cgaagcctac
     9121 ggcctgtttt taaaaggtga aagcgatctg gtactgagtt acaccacctc tccggcttat
     9181 cacattctcg aagagaaaaa agataactac gccgccgcga actttagcga aggtcactat
     9241 ctgcaggtgg aagtcgccgc ccgcaccgct gccagcaagc agccggagct ggcgcaaaaa
     9301 ttcctccagt ttatggtttc tccggctttt cagaatgcga tcccaaccgg taactggatg
     9361 tatccggtgg caaatgtcac gctgcctgcc gggtttgaac aattgaccaa accggccacc
     9421 acgctggagt tcacaccagc tgaagtggcg gcacaacgtc aggcatggat tagcgaatgg
     9481 caacgcgccg tcagccgtta attcccggct ggttaattcc aggtgtaagc gccgccacgc
     9541 tggtggtagc ggttgcgctc gcggcgtttc tcgccctgtg gtggaacgcg ccgcagggta
     9601 actgggtggc agtctggcag gacagctacc tgtggcatgt ggtgcgcttc tccttctggc
     9661 aggcgtttct ctcggcgcta ctctctgtcg tacccgcgat attcctcgcc cgcgccctct
     9721 atcgcaggcg ctttccgggt cggctggcgc tgttgcgtct gtgcgcaatg accttgatcc
     9781 tcccggtgct agttgctgtt ttcggcattc ttagcgtcta tggtcgtcag ggctggctgg
     9841 catcgctctg ccaatcgctc ggtctggagt ggaccttttc gccctacggc ctgcaaggta
     9901 ttttgctggc gcacgtattt tttaatctgc cgatggcgag ccgcttatta ctccaggcac
     9961 tggaaaacat tcccggcgaa caacgtcagc ttgccgccca gcttgggatg cgcggctggc
    10021 attttttccg cttcgtcgaa tggccgtggt tacggcgaca aatcccgccg gttgctgcac
    10081 ttatctttat gctctgtttc gccagcttcg ccaccgtgct atcactgggc ggcggtccgc
    10141 aggcgaccac tatcgagctg gctatttatc aggcgctgag ttacgactac gatcctgccc
    10201 gcgcggcgat gctggcgctg atccagatgg tgtgttgcct tgggctggta ctgttgagtc
    10261 agcgattgag taaggccatt gcgccaggca ccacgctgct gcaaggctgg cgcgacccgg
    10321 acgatcgtct gcatagccgc atttgcgaca cggtgttaat tgtgctggcg ctgctgctgt
    10381 tgctgccacc gttactggcg gtgatcgtcg atggcgtaaa tcgccagttg ccggaagtgc
    10441 tggcacaacc ggtgctgtgg caggcgctgt ggacctcgtt gcgtattgcg ctggcggcag
    10501 gtgtattgtg cgtagtgctg accatgatgc tgctatggag cagtcgcgaa cttcgggcgc
    10561 ggcagaaaat gctggcgggt caggcgctgg agatgagcgg catgttgatc ctcgccatgc
    10621 cggggattgt gctggctacc ggcttctttt tactgctcaa caacaccatt ggcctgccgc
    10681 aatctgctga cggcattgtg attttcacca atgcgttaat ggcgatccct tatgcgctga
    10741 aagtactgga aaacccgatg cgcgatatca ccgcccgcta cagtatgttg tgtcagtcgc
    10801 tgggcattga aggctggtcg cgcttaaaag tggtcgagct gcgcgccctg aaacgtccac
    10861 tggcgcaggc gctggctttt gcctgcgtgc tgtcgattgg tgattttggc gtagtggcgt
    10921 tgttcggtaa cgatgatttc cgtaccctgc cgttttatct ctaccagcaa attggctcct
    10981 atcgcagcca ggacggtgcg gtcaccgcgt taattctgct actactctgt tttctgctgt
    11041 ttaccgtgat tgaaaaacta ccggggcgaa atgttaaaac tgactgatat cacctggctt
    11101 tatcaccatt tgccgatgcg ttttagctta acggtagaac gcggcgagca ggtggcaatc
    11161 ctcgggccaa gcggcgcggg taaaagtacc ctgctgaatt tgatcgccgg ttttctgacg
    11221 ccagccagcg gttcgctgac tatcgacggc gttgatcaca ccacgacgcc gccgtcacgc
    11281 cgtccggtat cgatgctgtt tcaggagaac aacctgttca gccacctgac ggtcgcgcag
    11341 aacatcgggc tggggcttaa tccgggattg aagctgaacg cggcgcagca ggagaaaatg
    11401 cacgctatcg cccgacagat ggggattgat aatttaatgg cgcggttacc gggcgagctt
    11461 tccggcggtc agcgacagcg cgtggcgtta gcgcgttgtc tggtgcgcga acagccgatt
    11521 ttattgctcg atgaaccgtt ctctgcgctc gatccggcgt tacgtcagga gatgctgacg
    11581 ctggtgagca cgagctgcca gcagcaaaaa atgacgctat tgatggtgtc gcacagtgtg
    11641 gaagatgcgg cgcggatcgc cacacgctcg gtggtggtcg ccgacgggcg catcgcctgg
    11701 cagggtaaga ccaatgagtt gttgagcggt aaggcgagtg cttcggcact attggggatt
    11761 acgggttagt atgcactgtc ggatgcggcg tgaacgcctt atccgaccta cgggtagtgc
    11821 gttttgtagg cctgataaga cgcggtcagc gtcgcatcag gcaacccgca caagactatc
    11881 tcctaacccc caaccacttt acgcaaaata tcgatataca ccggcatcaa cgggtggcga
    11941 attaacacca ccagcgccac cacgccgatg gcagaaatta acggcgtcag ccacaacaaa
    12001 cggccgcgcg acaaataatg actcaaacgg tcagtcgctt taccactgcg ccataaccgc
    12061 cagcacagcc agccaccaac ccacaaaaac accgctgttg ccagcagcaa ccatttaaac
    12121 tcaccgctct gcattccggc tggaatatcg atcgccgcgc ccgccagaat ccctggcagg
    12181 aagtaaaacg gtggccacag caggcagccg ataatattcg gcgtaatgaa ttttgccacc
    12241 ggcagatcca gcattcccgc caccattggc accagcggac gcgtcgggcc aacaaaacga
    12301 ccgaccagaa tggtgaacat gctgtgttga tgcaacgcat gttcagtttt atcgagtaat
    12361 gctttgtttt tcttcagaaa tgaccagcga tgcaacggct ttttaaaacg ccagcccagc
    12421 cagaaggaga tccagtcgcc cagcaggcaa ccgacgatcc ctgccagcca ggcgtgccag
    12481 aaacttaact cgccgctgcc aatcagcgct cccagccccg ccatcagcac cgtaccgggt
    12541 agaatcaaac cgaccagcgc cagcgactcc agaaaggcca ccaacaccac cgccatcaat
    12601 gaatacacgg tagattgggt aataaagtgt tccagcaatg cttgcataat gtgcctgtca
    12661 aatggacgaa gcagggattc tgcaaaccct atgctactcc gtcaagccgt caattgtctg
    12721 attcgttacc aattatgaca acttgacggc tacatcattc acctttttct tcacaaccgg
    12781 cacggaactc gctcgggctg gccccggtgc attttttaaa tactcgcgag aaatagagtt
    12841 gatcgtcaaa accaacattg cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa
    12901 gcagcttcgc ctggctgata cgttggtcct cgcgccagct taagacgcta atccctaact
    12961 gctggcggaa aagatgtgac agacgcgacg gtgacaagca aacatgctgt gcgacgctgg
    13021 cgatatcaaa attgctgtct gccaggtgat cgctgatgta ctgacaagcc tcgcgtaccc
    13081 gattatccat cggtggatgg agcgactcgt taatcgcttc catgcgccgc agtaacaatt
    13141 gctcaagcag atttatcgcc agcagctccg aatagcgccc ttccccttgc ccggcgttaa
    13201 tgatttgccc aaacaggtcg ctgaaatgcg gctggtgcgc ttcatccggg cgaaagaaac
    13261 ccgtattggc aaatattgac ggccagttaa gccattcatg ccagtaggcg cgcggacgaa
    13321 agtaaaccca ctggtgatac cattcgcgag cctccggatg acgaccgtag tgatgaatct
    13381 ctcctggcgg gaacagcaaa atatcacccg gtcggcaaac aaattctcgt ccctgatttt
    13441 tcaccacccc ctgaccgcga atggtgagat tgagaatata acctttcatt cccagcggtc
    13501 ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc gccaccagat
    13561 gggcattaaa cgagtatccc ggcagcaggg gatcattttg cgcttcagcc atacttttca
    13621 tactcccgcc attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact
    13681 gcgtctttta ctggctcttc tcgctaaccc aaccggtaac cccgcttatt aaaagcattc
    13741 tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg
    13801 gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt
    13861 tatccataag attagcggat cctacctgac gctttttatc gcaactctct actgtttctc
    13921 catacccgtt tttttggatg gagtgaaacg atggcgattg caattggcct cgattttggc
    13981 agtgattctg tgcgagcttt ggcggtggac tgcgccagcg gtgaagagat cgccaccagc
    14041 gtagagtggt atccccgttg gcaaaaaggg caattttgtg atgccccgaa taaccagttc
    14101 cgtcatcatc cgcgtgacta cattgagtca atggaagcgg cactgaaaac cgtgcttgca
    14161 gagcttagcg tcgaacagcg cgcagctgtg gtcgggattg gcgttgacac aaccggctcg
    14221 acgcccgcac cgattgatgc cgacggtaac gtgctggcgc tgcgcccgga gtttgccgaa
    14281 aacccgaacg cgatgttcgt attgtggaaa gaccacactg cggttgaaga agcggaagag
    14341 attacccgtt tgtgccacgc gccgggcaac gttgactact cccgctatat tggcggtatt
    14401 tattccagcg aatggttctg ggcaaaaatc ctgcatgtga ctcgccagga cagcgccgtg
    14461 gcgcaatctg ccgcatcgtg gattgagctg tgcgactggg tgccagctct gctttccggt
    14521 accacccgcc cgcaggatat tcgtcgcgga cgttgcagcg ccgggcataa atctctgtgg
    14581 cacgaaagct ggggcggcct gccgccagcc agtttctttg atgagctgga cccgatcctc
    14641 aatcgccatt tgccttcccc gctgttcact gacacctgga ctgccgatat tccggtgggc
    14701 accttatgcc cggaatgggc gcagcgtctc ggcctgcctg aaagcgtggt gatttccggc
    14761 ggcgcgtttg actgccatat gggcgcagtt ggcgcaggcg cacagcctaa cgcactggta
    14821 aaagttatcg gtacttccac ctgcgacatt ctgattgccg acaaacagag cgttggcgag
    14881 cgggcagtga aaggtatttg cggtcaggtt gatggcagcg tggtgcctgg atttatcggt
    14941 ctggaagcag gccaatcggc gtttggtgat atctacgcct ggtttggtcg cgtactcggc
    15001 tggccgctgg aacagcttgc cgcccagcat ccggaactga aagcgcaaat caacgccagc
    15061 cagaaacaac tgcttccggc gctgaccgaa gcatgggcca aaaatccgtc tctggatcac
    15121 ctgccggtgg tgctcgactg gtttaacggc cgccgcacac cgaacgctaa ccaacgcctg
    15181 aaaggggtga ttaccgatct taacctcgct accgacgctc cgctgctgtt cggcggtttg
    15241 attgctgcca ccgcctttgg cgcacgcgca atcatggagt gctttaccga tcaggggatc
    15301 gccgtcaata acgtgatggc gctgggcggc atcgcgcgga aaaaccaggt cattatgcag
    15361 gcctgctgcg acgtgctgaa tcgcccgctg caaattgttg cctctgacca gtgctgtgcg
    15421 ctcggtgcgg cgatttttgc tgccgtcgcc gcgaaagtgc acgcagacat cccctcagcc
    15481 cagcaaaaaa tggccagtgc ggtagagaaa accctgcaac cgtgcagcga acaggcacaa
    15541 cgctttgaac agctttatcg ccgctatcag caatgggcga tgagcgccga acaacactat
    15601 cttccaactt ccgccccggc acaggctgcc caggccgttc cgactctata aggacacgat
    15661 aatgacgatt tttgataatt atgaagtgtg gtttgtcatt ggcagccagc atctgtatgg
    15721 cccggaaacc ctgcgtcagg tcacccaaca tgccgagcac gtcgttaatg cgctgaatac
    15781 ggaagcgaaa ctgccctgca aactggtgtt gaaaccgctg ggcaccacgc cggatgaaat
    15841 caccgctatt tgccgcgacg cgaattacga cgatcgttgc gctggtctgg tggtgtggct
    15901 gcacaccttc tccccggcca aaatgtggat caacggcctg accatgctca acaaaccgtt
    15961 gctgcaattc cacacccagt ttaacgcggc gctgccgtgg gacagtatcg atatggactt
    16021 tatgaacctg aaccagactg ctcatggcgg tcgcgagttc ggcttcattg gcgcgcgtat
    16081 gcgtcagcaa catgccgtcg ttaccggtca ctggcaggat aaacaagccc atgagcgtat
    16141 cggctcctgg atgcgtcagg cggtctctaa acaggatacc cgtcatctga aagtctgccg
    16201 atttggcgat aacatgcgtg aagtggcggt caccgatggt gataaagttg ccgcacagat
    16261 caagttcggt ttctccgtca atacctgggc ggttggcgat ctggtgcagg tggtgaactc
    16321 catcagcgac ggcgatgtta acgcgctggt cgatgagtac gaaagctgct acaccatgac
    16381 gcctgcaaca caaatccacg gcgaaaaacg acagaacgtg ctggaagcgg cgcgtattga
    16441 gctggggatg aagcgtttcc tggaacaagg tggcttccac gcgttcacca ccacctttga
    16501 agatttgcac ggtctgaaac agcttccagg tctggccgta cagcgtctga tgcagcaggg
    16561 ttacggcttt gcgggcgaag gcgactggaa aactgccgcc ctgcttcgca tcatgaaggt
    16621 gatgtcaacc ggtctgcagg gcggtacctc ctttatggag gactacacct atcacttcga
    16681 gaaaggcaat gacctggtgc tcggctccca tatgctggaa gtctgcccgt cgattgcagt
    16741 agaagagaaa ccgatcctcg acgttcagca tctcggtatt ggtggtaagg acgatcctgc
    16801 ccgcctgatc ttcaataccc aaaccggtcc agcgattgtt gccagcctga ttgatctcgg
    16861 cgatcgttac cgtctgctgg ttaactgtat cgacacggtg aaaaccccgc actccctgcc
    16921 gaaactgccg gtggcgaatg cgctgtggaa agcgcaaccg gatctgccaa ctgcttccga
    16981 agcgtggatc ctcgctggtg gcgcgcacca taccgtcttc agccatgcgc tgaacctcaa
    17041 cgatatgcgc cagttcgccg agatgcacga cattgaaatc acggtgattg ataacgacac
    17101 ccgcctgcca gcgtttaaag acgcactgcg ctggaacgaa gtgtattacg gatttcgtcg
    17161 ctaagtagcc acatcaggta tgtaacgcct gatgcgacgc tgacgcgtct tatctggcct
    17221 acgcgctgtg tttttgtagg ccggataagc aaagcgcatc cggcattcaa cgcctgatgc
    17281 gacgctggcg cgtcttatca ggcctacgcg ctgcgatttt gtagaccgga taagcaaagt
    17341 gcatccggca cgaaggagtc aacatgttag aagatctcaa acgcctggta ttagaagcca
    17401 acctggcgct gccaaaacac aacctggtca cgctcacatg gggcaacgtc agcgccgttg
    17461 atcgcgagcg cggcgtcttt gtgatcaaac cttccggcgt cgattacagc gtcatgaccg
    17521 ctgacgatat ggtcgtggtt agcatcgcaa ccggtgaagt ggttgaaggt acgaaaaagc
    17581 cctcctctga cacgccaact caccggctgc tctatcaggc attcccgtcc attggcggca
    17641 ttgtgcatac gcactcgcgc cacgccacca tctgggcgca ggcgggtcag tcgattccag
    17701 caaccggcac cacccacgcc gactatttct acggcaccat tccctgcacc cgcaaaatga
    17761 ccgacgcaga aatcaacggt gaatatgagt gggaaaccgg taacgtcatc gtagaaacct
    17821 tcgaaaaaca gggtatcgat gcagcgcaaa tgcccggcgt cctggtccat tctcacggcc
    17881 catttgcatg gggcaaaaat gccgaagatg cggtgcataa cgccatcgtg ctggaagagg
    17941 tcgcttatat ggggatattc tgccgtcagt tagcgccgca gttaccggat atgcagcaaa
    18001 cgctgctgga taaacactat ctgcgtaagc atggcgcgaa ggcatattac gggcagtaat
    18061 gactgtataa aaccacagcc aatcaaacga aaccaggcta taatcaagcc tggtttttta
    18121 ttggattttc aacgtggcgc aggcaggttt tatcttaacc cgacactggc gggatacccc
    18181 gcaagggaca gaagtctcct tctggctggc gacggacaac gggccgttgc aggttacgct
    18241 tgcaccgcaa gagtccgtgg cgtttattcc tgccgatcag gttccccgcg ctcagcatat
    18301 tttgcagggt gaacaaggct ttcgcctgac gccgctggcg ttaaaggatt ttcaccgcca
    18361 gccggtgtat ggcctttact gtcgcgccca tcgccagttg atgaattacg aaaagcgcct
    18421 gcgtgaaggt ggcgttaccg tttacgaggc cgatgtgcgc ccgccagaac gctatctgat
    18481 ggagcgtttt atcacctcgc cagtgtgggt cgagggtgat atacgcaatg gcgctatcgt
    18541 taatgcccgt ctgaaaccgc atcccgacta tcgtccgccg ctcaagtggg tttctattga
    18601 tattgaaacc acccgccacg gtgagctgta ctgcatcggc ctggaaggtt gcgggcagcg
    18661 catcgtttat atgctggggc cagagaatgg cgacgcctcc gcgctcgatt ttgaactgga
    18721 atacgtcgcc agtcgcccgc agctactgga aaaactcaac gcctggtttg ccaactacga
    18781 tcctgatgtg atcatcggtt ggaacgtggt gcagtttgat ctgcgaatgc tgcaaaaaca
    18841 tgccgagcgt tatcgtattc cgctgcggct ggggcgcgat aacagtgagc tggagtggcg
    18901 cgagcacggc tttaaaaacg gtgtgttttt tgctcaggct aaaggtcggc taattatcga
    18961 cggtatcgag gcgctgaaat ccgcgttctg gaatttctct tcattctcgc tggaaactgt
    19021 cgctcaggag ctattaggcg aaggaaaatc tatcgataac ccgtgggatc gaatggacga
    19081 aattgaccgc cgtttcgccg aagataaacc tgcgctggcg acttataacc tgaaagattg
    19141 cgagctggtg acgcagattt tccataaaac tgaaatcatg ccgtttttgc tcgaacgagc
    19201 aacggtgaac ggcctgccgg tagaccgcca cgggggatcg gtggcggcgt ttggtcatct
    19261 ctattttccc cgtatgcacc gcgctggtta tgtcgcgcct aatctcggcg aagtgccgcc
    19321 acacgccagc cctggcggct acgtgatgga ttcacggcca gggctttatg attcagtgct
    19381 ggtgctggac tataaaagcc tgtacccgtc gatcatccgc acctttctga ttgatcccgt
    19441 cgggctggtg gaaggcatgg cgcagcctga tccagagcac agtaccgaag gttttctcga
    19501 tgcctggttc tcacgagaaa aacattgcct gccggagatt gtgactaaca tctggcacgg
    19561 gcgcgatgaa gccaaacgcc agggtaacaa accgctgtcg caggcgctga aaatcatcat
    19621 gaatgccttt tatggcgtgc tcggcaccac cgcctgccgc ttcttcgatc cgcggctggc
    19681 atcgtcgatc accatgcgtg gtcatcagat catgcggcaa accaaagcgt tgattgaagc
    19741 acagggctac gacgttatct acggcgatac cgactcaacg tttgtctggc tgaaaggcgc
    19801 acattcggaa gaagaagcgg cgaaaatcgg tcgtgcactg gtgcagcacg ttaacgcctg
    19861 gtgggcggaa acgctgcaaa aacaacggct gaccagcgca ttagaactgg agtatgaaac
    19921 ccatttctgc cgttttctga tgccaaccat tcgcggagcc gataccggca gtaaaaagcg
    19981 ttatgccgga ctgattcagg agggcgacaa gcagcggatg gtgtttaaag ggctggaaac
    20041 cgtgcgcacc gactggacgc cgctggccca gcagtttcag caggagctat acctgcgtat
    20101 cttccgtaac gagccatatc aggaatacat ccgcgaaacc atcgacaaac tgatggcggg
    20161 tgaactggat gcgcggctgg tttaccgtaa acgccttcgc cgtccgctga gcgaatatca
    20221 gcgtaatgtt ccgcctcatg tacgcgccgc tcgccttgcc gatgaagaaa accaaaagcg
    20281 cggtcgcccc ttgcaatatc agaatcgcgg caccattaag tacgtatgga ccaccaacgg
    20341 cccggagccg ctggactacc aacgttcacc actggattac gaacactatc tgacccgcca
    20401 gctacaaccc gtggcggagg gaatactccc ttttattgag gataattttg ctacacttat
    20461 gaccgggcaa cttgggctat tttgagcaaa aaaaagagtt cgccagatac cattttgatg
    20521 cgtgacgaat gctttgccat ccagtaccat agcgcccttt ccattcctgg acctgaataa
    20581 cgccactacc tcataagcac ggtagcgggt ggttattgcc tgcaattaaa gatatagagc
    20641 cgaacacata tgccttttac acttggtcaa cgctggatca gcgatacaga aagcgaattg
    20701 ggacttggaa ccgttgtcgc ggtggatgcg cgaactgtca ctttactttt cccatctact
    20761 ggtgaaaacc gtctgtacgc acgcagtgat tcccccgtga cccgcgtgat gttcaaccct
    20821 ggtgatacca ttaccagcca tgacggctgg cagatgcaag tcgaagaagt aaaagaagaa
    20881 aatggcttgc tgacctatat cggtactcgc ctggatactg aagagtccgg cgtagccctg
    20941 cgtgaagttt tccttgatag caaactggtg ttcagcaaac cgcaggaccg tctgtttgcc
    21001 gggcagattg accgtatgga ccgctttgcg ctgcgttatc gcgcgcgtaa gtattccagc
    21061 gaacagttcc gtatgccgta cagcggcctg cgcggtcagc gtaccagcct gattccgcat
    21121 cagctcaaca tcgctcatga tgttggtcgc cgccacgcgc cgcgcgtcct gctggctgac
    21181 gaagtgggtt tagggaaaac cattgaagcc gggatgatcc tgcatcagca actgctctct
    21241 ggcgctgctg aacgtgtgct gattatcgtc ccggaaacct tacagcatca gtggctggta
    21301 gaaatgctgc gccgtttcaa cctgcgcttt gcgctatttg atgatgagcg ttatgccgaa
    21361 gctcagcacg atgcttacaa cccgtttgac accgaacagc tggtgatttg ctcgctggat
    21421 tttgcccgtc gtagcaaaca gcgcctggaa catctctgtg aagccgaatg ggacctgctg
    21481 gtggtcgatg aagcgcatca cctggtgtgg agcgaagatg cgccaagccg tgaatatcag
    21541 gccattgaac aactggcaga gcacgtgccg ggcgttctgc tgctgaccgc gaccccggaa
    21601 cagctgggga tggaaagcca cttcgcccgt ctgcgtctgc tggacccgaa ccgtttccac
    21661 gattttgcgc agttcgttga agagcagaaa aattatcgtc cggttgcgga cgccgttgcc
    21721 atgctgctgg caggtaacaa actgagcaat gacgaactga acatgctcgg cgagatgatc
    21781 ggcgagcagg atatcgagcc gctgttgcag gcagcaaaca gcgacagcga agatgcccag
    21841 agcgcccgtc aggagctggt ttcgatgctg atggatcgcc acggcaccag ccgcgtgctg
    21901 ttccgtaaca cgcgtaacgg tgtgaaagga ttcccgaaac gcgagctgca caccattaaa
    21961 ctgccgctac cgacgcagta tcagacggct attaaagtct ccggcattat gggcgcacgt
    22021 aaaagtgcgg aagatcgtgc tcgcgatatg ctctacccgg agcgtattta tcaggaattt
    22081 gaaggtgata acgccacctg gtggaacttc gatccgcgcg ttgagtggct gatgggctac
    22141 ctgaccagcc atcgctctca gaaagtgctg gtgatctgcg ccaaagctgc cactgcgctg
    22201 caactggagc aggtactgcg cgaacgtgaa ggtattcgcg ctgcggtgtt ccacgaaggt
    22261 atgtcgatta tcgaacgtga ccgcgctgcc gcctggtttg ccgaagaaga caccggcgca
    22321 caggtactgc tgtgctcaga aatcggttct gaaggacgta acttccagtt cgccagccac
    22381 atggtgatgt ttgacctgcc attcaacccg gatctactgg agcagcgtat tggtcgtctg
    22441 gatcgtatcg gccaggcgca cgatattcag atccatgtgc cttatctgga gaaaaccgct
    22501 cagtcggtgc tggtgcgctg gtatcacgaa ggtctggatg catttgagca cacctgcccg
    22561 accggacgca ctatttacga tagcgtatac aacgatctga ttaactatct ggcttcaccg
    22621 gatcaaaccg aaggctttga cgatctgatc aaaaactgcc gcgagcaaca tgaagcgctg
    22681 aaagcacagc tggaacaggg tcgtgaccgc ctgctggaaa tccactccaa cggtggcgaa
    22741 aaagcccagg cactggcaga aagcattgaa gagcaggatg acgataccaa cctgatcgcc
    22801 ttcgccatga acctgttcga tattatcggt atcaatcagg acgatcgcgg cgacaacatg
    22861 atcgtgctga cgccgtccga tcatatgctg gtgccggact tccctggcct gtcggaagat
    22921 ggcatcacca tcacctttga tcgtgaagtg gcgctggcgc gtgaagatgc acagtttatt
    22981 acctgggagc atccgctgat ccgcaacggt ctggatctga ttctttctgg cgataccggt
    23041 agcagcacga tttcactgtt aaaaaacaaa gcgttgccgg taggtacgct gttggtggaa
    23101 ctgatttatg tggttgaagc ccaggctccg aagcagttgc agctcaaccg cttcctgcca
    23161 ccgacgccgg tacgtatgct gctggataaa aacggcaaca acctggcggc gcaggtagag
    23221 tttgaaacct ttaaccgcca gcttaacgcg gttaaccgtc acaccggcag caaactggtt
    23281 aacgccgtgc agcaggatgt tcacgctatc cttcaactgg gtgaagcgca gatcgagaaa
    23341 tctgcccgtg cattgattga tgcagcgcgt aacgaagccg acgaaaaact gtctgccgag
    23401 ctgtctcgtc tggaagctct gcgtgcagtg aacccgaaca ttcgtgacga cgaactgacc
    23461 gccattgaga gcaaccgtca gcaggtaatg gaaagcctgg atcaggcagg ttggcgtctg
    23521 gatgccctgc gtttgatcgt tgtaacgcat cagtaacgga gccgaaaatg gggatggaaa
    23581 actacaatcc accgcaggaa ccctggttgg ttatcctgta tcaggatgac catattatgg
    23641 tggtcaacaa gccgagcggt ttgttgtcag tgccgggtcg tctggaagag cacaaagaca
    23701 gcgtgatgac gcgcattcag cgtgattatc cgcaggcaga atcggtgcat cgtctggata
    23761 tggctaccag cggcgtgatt gtagtggcgc tgaccaaagc cgcggagcgg gagttaaaac
    23821 gccagttccg cgagcgcgag ccgaaaaagc agtatgtggc ccgcgtctgg gggcatccat
    23881 cccccgcaga aggtctggtg gatctgccgc tgatttgcga ctggccaaac cgcccgaaac
    23941 agaaagtctg ttacgaaacg ggtaaacctg cgcagacgga atatgaagtg gtggagtatg
    24001 cggcggataa cacggcaaga gtggtgttaa aaccgattac cgggcgttcg catcaattgc
    24061 gtgtgcatat gctggcgctg gggcatccga ttctcggcga tcgtttttat gcatcaccag
    24121 aagcgagagc gatggcaccg cgtttgttgc tacatgcaga gatgttgacg attacccatc
    24181 cggcgtatgg caatagtatg acgtttaaag cgccagcgga tttttaatcg tcacgctggc
    24241 gcagttcggt aggcggataa ggcgttcacg ccgcatccgg caattgtgcg ccgattgcct
    24301 gatgcgacgc gggccgcgtc ttatcaggcc tacagtttaa aacttattta aaccctttct
    24361 gctgctttat cagctcatat gcctgctgaa tttcctgcgc tttctgcttc gccatctcca
    24421 tcatctcagg cggcaaacct ttcgccacca gcttatcggg atggtgttca ctcatcagct
    24481 tacggtaggc acgtttgatg gtggtcgcat catccgtcgg cttcacgccc agcacattac
    24541 aggcatcttc cagcgttggg ccacgctgcg cttgctgcca gttaccaccg ccagtttgct
    24601 gctgataacc gccgccaaac tgtgcaccgc cctgcatcat gcgcaaaaac tggtcaaact
    24661 gagcgcggga gatccctaat tcttctgcaa tgacatacag caccgcccgt tcattcgggt
    24721 gcagtgaacc atcagcaaac gccgcctgaa tctggatctc cagaaacata cgaattaagt
    24781 caaaacgacc aaagcagaca ctgcgaaact ggcgcatctt ttcgcgcagc gggtaattgt
    24841 ctgattttcc cacccggaac gcattttgcg ccgcagtacg ggaagcgcca tgaagattca
    24901 ttcggtccat caactggctg gcgatatgaa tatcagcctc cgtgacgcga cctttggatt
    24961 tggttaaatg ccccatcact tcaaaagtgg tggcaaaaaa cagcgcctga cgctcacgct
    25021 ggttggcgaa ccacgccatt ttacggctac gggctttatc aaacatatgg ccaattaaca
    25081 ggcctaacac tacgccccaa aagccgccgc ccatcagtaa ggccacggcc acgccaatga
    25141 tttttcccca atactgcata tattccccaa atcgacacac ggatatcagg gctatctccc
    25201 acaatataaa ggtgctttta ccgttttccg gctgcggtca attgtgggac atcgcctata
    25261 atttgcatta tcatacctgt cattcaatgc cgtgcctaac accactgacg ctattcggac
    25321 aggattaaca ctagcgtaga gatgacgagt acgttagtct ctgaccgttt gtcacgcgca
    25381 acgttaccga tgatggaaca ataaaatcaa cgtatgaaaa aacgtatccc cactctcctg
    25441 gccaccatga ttgccaccgc cctttatagt caacagggac tggcagccga cctcgcctca
    25501 cagtgcatgt tgggcgtgcc aagctatgac cgtcctctgg tacagggcga taccaatgac
    25561 ttacccgtga ctatcaatgc tgaccacgcg aaaggggact acccggatga cgccgtgttt
    25621 actggcagcg tggatatcat gcagggtaac agccgtctgc aggccgacga agtgcagctc
    25681 catcaaaaag aggcaccagg acaaccggag ccggtacgta ccgttgatgc gctcggtaat
    25741 gtccattacg acgataacca ggtgatcctc aaagggccga aaggctgggc gaatctgaac
    25801 accaaagata ccaacgtctg ggaaggtgat taccagatgg tgggtcgcca gggtcgcggt
    25861 aaagcggacc tgatgaaaca acgtggcgaa aaccgctata ccattctgga taacggtagc
    25921 tttacctcct gtctgccggg ttctgacacc tggagcgtgg taggtagcga aattattcat
    25981 gaccgcgaag aacaagttgc ggagatctgg aacgcccgct ttaaggtggg tccggtaccg
    26041 atcttttata gcccctattt gcagttgccg gtgggtgaca aacgtcgctc tggtttcttg
    26101 atcccgaacg ccaagtacac caccaccaac tactttgagt tctacctgcc atattactgg
    26161 aacatcgcgc caaatatgga tgccaccatc acgccgcatt atatgcatcg tcgtggcaac
    26221 atcatgtggg agaacgaatt ccgctacctc tcccaggcgg gcgctggctt gatggaactg
    26281 gactatctgc cttcagataa agtctatgaa gatgaacacc cgaacgatga cagttcacgt
    26341 cgttggttat tctactggaa ccactccggg gtcatggatc aggtgtggcg tttcaacgtc
    26401 gactacacca aggtcagcga tcctagctac ttcaatgatt tcgataacaa gtacggttcc
    26461 agtactgacg gctacgcaac gcaaaaattc agcgttggct atgcggtgca aaacttcaat
    26521 gccaccgttt caaccaagca gttccaggtt ttcagcgaac agaacaccag tagctactcg
    26581 gcagagccgc agttagacgt taattactac cagaatgatg ttggtccgtt tgatacgcgt
    26641 atttacggcc aggcagtgca ctttgttaac accagagacg acatgcctga agcaacccgt
    26701 gttcacctgg aaccgaccat caatttgccg ctctctaata actggggcag catcaatacc
    26761 gaagcgaagt tgctggcaac ccattatcag caaactaatc ttgactggta taactccaga
    26821 aacacgacca agctggacga atccgttaac cgcgtaatgc cgcaattcaa agttgacggc
    26881 aaaatggtct ttgaacgcga tatggaaatg ctggctccgg gttataccca aacgctggaa
    26941 ccgcgcgcgc agtatttgta cgtgccgtat cgcgatcaga gcgacatcta taactacgac
    27001 tcatctctgc tgcaatctga ctactctggc ctgttccggg accggactta cggcggtctt
    27061 gaccgtattg cctccgctaa ccaggtgacg accggtgtca catctcgcat atatgatgat
    27121 gctgccgttg aacgttttaa tatttccgtt ggtcaaatct actatttcac ggagtctcgc
    27181 actggcgatg acaacataac atgggagaat gacgacaaaa cgggctcact ggtgtgggca
    27241 ggcgatactt actggcgtat ctccgagcgt tggggattgc gtggcgggat tcagtacgat
    27301 acacgtctgg ataacgtagc gaccagtaac tccagcattg aataccgtcg ggatgaagac
    27361 cgtctggtac agctgaatta ccgttacgcc agcccggaat atattcaggc tacgctgcct
    27421 aagtactatt ccactgctga gcaatataag aatggtattt cgcaggtagg tgctgtcgcc
    27481 agctggccaa ttgccgatcg ttggtccatt gttggggcct actactacga caccaatgcg
    27541 aacaagcaag ctgactctat gttaggtgtg caatacagct cctgctgcta tgcaattcgc
    27601 gtcggttacg agcggaagct gaacggttgg gataacgata aacaacatgc ggtatatgac
    27661 aacgcaatcg gctttaacat cgaacttcgc ggcctgagct ccaactacgg tctgggtacg
    27721 caagagatgc tgcgttcgaa cattctgccg tatcaaaact ctttgtgatt tgttgattta
    27781 ccacgtaatc cgcagtgcgg ttaattgaaa tggaaaaagt atgaagaact ggaaaacgct
    27841 gcttctcggt atcgccatga tcgcgaatac cagtttcgct gccccccagg tagtcgataa
    27901 agtcgcagcc gtcgtcaata acggcgtcgt gctggaaagc gacgttgatg gattaatgca
    27961 gtcggtaaaa ctgaacgctg ctcaggcaag gcagcaactt cctgatgacg cgacgctgcg
    28021 ccaccaaata atggaacgtt tgatcatgga tcaaatcatc ctgcagatgg ggcagaaaat
    28081 gggagtgaaa atctccgatg agcagctgga tcaggcgatt gctaacatcg cgaaacagaa
    28141 caacatgacg ctggatcaga tgcgcagccg tctggcttac gatggtctga actacaacac
    28201 ctatcgtaac cagatccgca aagagatgat tatctctgaa gtgcgtaaca acgaggtgcg
    28261 tcgtcgcatc accatcctgc cgcaggaagt cgaatccctg gcgcagcagg tgggtaacca
    28321 aaacgacgcc agcactgagc tgaacctgag ccacatcctg atcccgctgc cggaaaaccc
    28381 gacctctgat caggtgaacg aagcggaaag ccaggcgcgc gccattgtcg atcaggcgcg
    28441 taacggcgct gatttcggta agctggcgat tgctcattct gccgaccagc aggcgctgaa
    28501 cggcggccag atgggctggg gccgtattca ggagttgccc gggatcttcg cccaggcatt
    28561 aagcaccgcg aagaaaggcg acattgtggg tccgattcgt tccggcgttg gcttccatat
    28621 tctgaaagtt aacgacctgc gcggcgaaag caaaaatatc tcggtgaccg aagttcatgc
    28681 tcgccatatt ctgctgaaac cgtcgccgat catgactgac gaacaggccc gcgtgaaact
    28741 ggaacagatt gctgctgata tcaagagtgg taaaacgact tttgctgccg cagcgaaaga
    28801 gttctcgcag gatccaggtt ctgctaacca gggcggcgat ctcggttggg caacagcgga
    28861 catttttgat ccggctttcc gtgacgccct gacccgcctg aacaaaggtc aaatgagtgc
    28921 accggttcac tcttcattcg gctggcattt aatcgaactg ctggataccc gtaatgtcga
    28981 taaaaccgac gctgcgcaga aagatcgtgc ataccgcatg ctgatgaacc gtaagttctc
    29041 ggaagaagca gcaagctgga tgcaggaaca acgtgccagc gcctacgtta aaatcctgag
    29101 caactaatgg ttaaaaccca acgtgttgtg atcactcccg gcgagcccgc cgggattggc
    29161 ccggacttag ttgtccagct tgcacagcgt gagtggccgg tcgaactggt tgtttgtgcc
    29221 gatgccactc tccttaccga ccgggcagcg atgctcggtt tgccgctcac cctccgcact
    29281 tattccccca actctcctgc acaaccgcaa actgcgggca cattaacgct acttcctgtc
    29341 gcgctacgtg aatctgtcac tgcggggcag ttagcggttg aaaatgggca ttatgtggtg
    29401 gaaacgctgg cgcgagcgtg cgatggctgt ctgaacggtg aatttgctgc gctgatcaca
    29461 ggccccgtgc ataaaggcgt cattaacgac gcaggcattc cgtttaccgg tcataccgag
    29521 tttttcgaag agcgttcgca ggcgaaaaaa gtggtgatga tgctggcgac cgaagaactt
    29581 cgcgtggcgc tggcaacgac gcatttaccg ctgcgcgata tcgcagatgc tatcacccct
    29641 gcgcttttgc acgaagtgat tgctattttg catcacgatt tgcggaccaa atttggtatt
    29701 gccgaaccgc gcattctggt ctgcgggctg aatccacacg cgggcgaagg cggtcatatg
    29761 ggtacggaag agatagacac cattattccg ctgctcgacg agttgcgggc gcaggggatg
    29821 aaacttaacg ggccgctgcc tgccgatacc ctgtttcagc cgaaatatct cgataacgcc
    29881 gacgccgtgc tggcgatgta ccacgatcag ggtcttcccg tgctaaaata ccagggcttc
    29941 gggcgcggtg tgaacattac gctgggcctg ccctttattc gcacatcagt ggaccacggc
    30001 accgcgcttg aactggcggg acgtggcgaa gccgatgtcg gcagttttat tacggcgctt
    30061 aatctcgcca tcaaaatgat tgttaacacc caatgaataa tcgagtccac cagggccact
    30121 tagcccgtaa acgcttcggg caaaactttc tcaacgatca gttcgtgatc gacagcattg
    30181 tttctgccat taacccgcaa aagggccagg caatggtcga aatcggcccc ggtctggcgg
    30241 cattgaccga gccggtcggc gaacgtctgg accagctgac ggtcatcgaa cttgaccgcg
    30301 atctggcggc acgtctgcaa acgcatccat tcttaggccc gaaactgacg atttatcagc
    30361 aggatgcgat gacctttaac tttggcgaac tggccgagaa aatgggtcag ccgctgcgtg
    30421 ttttcggcaa cctgccttat aacatctcca cgccgttgat gttccatctg tttagctata
    30481 ctgatgccat tgccgacatg cactttatgt tgcaaaaaga ggtggtgaat cgtctggttg
    30541 caggaccgaa cagcaaagcg tatggtcgat taagcgtcat ggcgcaatac tattgcaacg
    30601 tgatcccagt gctggaagta ccgccgtcag cctttacacc accacccaaa gtggattccg
    30661 ccgtcgtgcg cctggttcct catgcaacga tgcctcaccc ggttaaagat gttcgtgtgt
    30721 tgagccgcat caccaccgaa gcctttaacc agcgtcgtaa aaccattcgt aacagcctcg
    30781 gcaacctgtt tagcgtcgag gtgttaacgg gaatggggat cgacccggcg atgcgagcgg
    30841 aaaatatctc tgtcgcgcaa tattgccaga tggcgaacta tctggcggag aacgcgcctt
    30901 tgcaggagag ttaacgatga tcaattcgcc ccgagtgtgt attcaggttc aaagcgtcta
    30961 cattgaggca caatcttcac ctgataatga acgttacgtt tttgcttata ccgtaaccat
    31021 acgcaatctg gggcgagcgc cagtgcagtt gttggggcgt tactggctga tcaccaatgg
    31081 caatggccgt gaaaccgaag tccagggcga aggagtggtt ggcgtccagc cacttatcgc
    31141 gcctggcgaa gagtaccagt acaccagcgg tgcaatcatt gaaaccccgc tgggcaccat
    31201 gcagggtcac tacgaaatga tcgatgaaaa tggcgtccct ttcagcatcg acattcccgt
    31261 attccgactc gccgttccca cactcattca ttaaaagaat atggcgacat accttattgg
    31321 cgacgttcat ggttgttacg atgaactgat cgcattgctg cataaagtag aatttacccc
    31381 tgggaaagat accctctggc taacgggcga tctggtcgcg cgcgggccgg gttcgctgga
    31441 tgttctgcgc tatgtgaaat ccttaggcga cagcgtacgt ctggtgctgg gcaatcacga
    31501 tctgcatctg ctggcggtat ttgccgggat cagccgcaat aaaccgaaag atcgcctgac
    31561 accgctgctg gaagcgccgg atgccgacga gctgcttaac tggctgcgtc gccagccttt
    31621 gctgcaaatc gacgaagaga aaaagttggt gatggcccac gcagggatca cgccgcagtg
    31681 ggatctacag accgccaaag agtgcgcgcg cgatgtagaa gcggtgctat cgagtgactc
    31741 ctatcccttc tttctcgacg ctatgtacgg cgatatgcca aataactggt caccggaatt
    31801 gcgggggctg ggaagactgc gttttatcac caacgctttt acccgtatgc gtttttgctt
    31861 cccgaacggt caactggata tgtacagcaa agaatcgccg gaagaggccc ctgccccact
    31921 gaaaccgtgg tttgcgattc ctggccctgt cgctgaagaa tacagcatcg cctttggtca
    31981 ctgggcatcg ctggagggca aaggtacgcc ggaaggtata tacgcgctgg ataccggctg
    32041 ctgctggggt ggtacattaa cctgcctgcg ctgggaagat aaacagtatt ttgtccagcc
    32101 gtcgaaccgg cataaggatt tgggcgaagc ggcggcgtct taaacacagc ctgatatagg
    32161 aaggccggat aagacgcgac cggcgtcgca tccggcacta gccgtaaatt ctatacaaaa
    32221 ttaccgccgc tccagaatct caaagcaata gctgtgagag ttctgcgcat cagcatcgtg
    32281 gaattcgctg aataccgatt cccagtcatc cggctcgtaa tccgggaaat gggtgtcgcc
    32341 ttccacttct gcgtcgatat gcgtcagata cagtttttgc gcttttggca agaactgttc
    32401 ataaacgcga ccgccgccaa tcaccatgat ttctggtacg tcaccacacg ccgcgatggc
    32461 ttcatccacc gacttcaccc acgttacgcg atcgtccgta cccggttgac tgctgaggat
    32521 aatatttttg cgtcctggca acggacgacc gattgattcc caggtatggc ggcccataat
    32581 cacgggttta tttaaggtgt tgcgtttaaa ccaggcgaga tcggcaggca ggttccacgg
    32641 catggcgttt tccatgccga taacgcgatc taccgctaac gccgcaatca gactgatcat
    32701 tgagatttcc cgataaaaaa aattgtcgcc actatacgta aagcgtaaac cgtcgtcgac
    32761 tggtgcgagg atgatgttga ggaaaatttt atattctgct ggcgagtcca cgctctctcc
    32821 gtggactcgc cgcattacaa tgaaacaaaa acaaacagtt agctgtaaag tgtgatttac
    32881 gtcactcttt attaggatga gggtttcgtt tccggttcat ccgccatgtt gccggtatgt
    32941 ttaccttctt cggttccctg ccagccatgt cgttgaatta atgacagatg ttcgcggtcc
    33001 tcggtaatga tctcacttaa catcgcgctg gtgcgtttat agaccgccgc gcgggctttg
    33061 gtgtcgttct caaccattgc catctcttcc accatctgaa tattaaagcg gcggaacaca
    33121 tcggcacgtt ctcgcgcttc atacggcccc agacctaaac tttccagtgc cagacgcccg
    33181 gttttcagcg caccttcgaa ggtttcacgc tccggctttt caacgcctgc ctgacgcaaa
    33241 cgaatgtagt ggtcgacatc gcgggcgcgg gcaataatct gcaaatgcgg gaaatgttct
    33301 ttcaccatct ctgtcagttg caggttggtt tgcggatcgt cgatggcgtt aatcagcact
    33361 tccgctttcg ccgctccggc agattccagt aaatccatcc gcgtggcatc gccataaaac
    33421 actttcatac caaatttacg caaggtttcg atatggtccg gatcgtgatc gagtaccacc
    33481 attttcaccc cgctggagag cagtaaacgt ccggtaatct gcccaaaacg accgaatccg
    33541 gcgataatca cgcgcggctg ttcttcgtcg atctcatcgg cttcacgcgc ttcctcagta
    33601 gaagattgct caaggcgatt gaggatcacc agcagaatcg gcgttgctgc catcgacagc
    33661 gccaccgcca gggtcagcga tttcgcccac tccggctcca gcacattcgc catctgcgcc
    33721 gcgccaaata ccacaaaggc aaactcactg ccctgcccta acaacaccgc aaaccaacga
    33781 cgctgtttat ttggcacttg caacggtcgg gcaatcagcc acagcatggc gattttgatg
    33841 atgaggaaac cgagcagcaa aatgacaatg cgcaatgggt tttcaagcag cgtgccaaag
    33901 tctatcgaca tgccaacacc gatgaaaaac agccccaaca gcaaaccttt aaatggttcg
    33961 atatcgctct ccagcgcatg acggtattcc gagcttgcca gcagtacgcc cgccagaaac
    34021 gcgcccatcg ccatcgacaa gccgacctct tccagcagca aaccaaagcc aaacacgagg
    34081 aataacgcca cggcactaaa cacttcccgc aagccagagc gggctacaaa acgcagcgcc
    34141 ggacgcgtga catagcgccc cagcaatacc accagcacca gcgcacccgc cacttttaac
    34201 gccgagagag caaatgcgcc catcatcgtc gaggcactgc tcgttgccag tagcggaatc
    34261 atcgccacca gcgggatcgc cgcgatatcc tggaacagca gcaccgcaaa ggcactgcga
    34321 cccatttgcg tcaccatcag attgcgttca ttcatcgcct gcatggcaat cgccgtagag
    34381 gagagcgcca gcgtcatgcc gatcaattcc gcgacctgcc agcgcagccc aagtaacatg
    34441 cagaacagcc ccagcaggcc accgcaaatc accatctgca atgcgccacc gccgaacact
    34501 gccgcacgca gcttccacag cctttgtgga tcgagttcga ggccgataat aaacagcatc
    34561 agcaccacgc caatctcggc aaagtgcaga atagattcgg catcggtcac cagtcgcagc
    34621 ccccacgggc caataatgca gccggcgatc aggtagccaa gtaccgatcc cagaccaaga
    34681 cgtaccgcaa tgggtacaat cagcgctgcc gaaccgagat aaatcagcgc ctgaatcagc
    34741 gtatggctat ccatgatggg cctcctgcca ttccagcaga cgttgcttat agtgacgcgc
    34801 ctgcccttcg agggtttcgt cgtcacaaat aaaggtgcag tgcatggcaa acggtggcag
    34861 ccagttcagc ccgcagtaga ttgccgtcgc ctgtagcggc tgcgacagca catcaaagcc
    34921 cggatgcgca ccaatttcaa aatggctttc cccgccgccg gtcgtcaccg cccacagcaa
    34981 atgtttgcca tgcagcgccg tgccgccgtg accgtaagcc cagccgtgcg agaaaacttt
    35041 atcgatccaa agtttgagga gcggtggaat gctgtaccac tgcatcgggt gctgccagac
    35101 gatcagatcg gcgcgagaca gcgcctcctg ctcggcggca atatcgatat tgaagtcagg
    35161 atagagttga taaagagagc gaatttcgac gccttccagc gtccttgcct gttcaagcat
    35221 ccgtttattc gcatgggaat gatgcggata cggatgcgca taaattataa gaatcatagg
    35281 tagcccgcgt gattacgcct ttgttttccc acaaagtgta gtcatatatt cacgcggctt
    35341 atagtcaata atattgattc agaaaagaga gaaatctgaa caaattaccc cattctctgc
    35401 ccgcggatcg tcatattgct ggtctgcgcc agtgacatcc ctcgagtctc cggggcaaac
    35461 gctacggaaa tcaacaagcc aaacagcgag atacccgccc ccattagcat cgtgttactg
    35521 ataccgtaat tattgataaa gatcggcagt gcccaggtcg aaacaatggt gccaatacgg
    35581 cttaaggaca taatcacgcc cacggcagag gcgcggatat ctgtcgggaa gagttcatta
    35641 ggatagagcc actgcaaatt acccggcccg ccagagaaaa aggcatacac cgcaaaggcc
    35701 atcactacca gccagatccc catatccggg atcagcccca aaaccgccag cgccagcgtc
    35761 atcatggcaa agctgccaat caacaatgga cgccgtccgg cagtgtttaa ccacagcatc
    35821 ggcggaatac agccgagcat aaagaacagg ctaatcacca cgttccccag tgccgcgttt
    35881 ttgccaaccc ccaatcccaa caaaccaacg atttgcgggc caaaggtgta aatggcgaac
    35941 attgggatca cctggcaggt ccagatggcg gcaacaaaca gaacaaaagg aaaatggcgg
    36001 cgattaaaca gatcgcgaaa acgggtttgc tgcggctgct cttcatcgaa agccaccggt
    36061 tcgccaaaca gttttatcat catctcttcg cactctttta ctcgcccttt gcgtaataac
    36121 cagcggggag attcaggcag ttcgaatcga ccaatcaaaa tcaacaaaca ggggatcgcc
    36181 gcgctaccca gcatccagcg ccagccgcct tccacatcat aaagccagta gccgaccaga
    36241 tcggcgcagg tcgcgccgac ataccacatg gcggcgataa agctgatgga aaacgcccgc
    36301 tgacgggtac tggagaactc ggtgatcatt gaggtggcga tgggataatc tgcaccgatg
    36361 acaatgccga taagtacccg catcaccaac agttcgacag gggatgagac aaacatcgtc
    36421 gccaccgata tcacgccgat ggcgatgata tcaatgagga acattttgcg ccgtccgact
    36481 ttatcggaga tatagccaaa cagcgatgtg ccaacgaaca gcccggcgag cgttcccgca
    36541 cccagcaagc caatccagtc agcgtccagt ttcagcgccg gagtcagttg ctccagcgcc
    36601 acgccaatca ttaccagtac ataaccatcc agaaacggac caccgcttcc ccacagcaaa
    36661 atgcggcggt gaatagagga gaatttgaga tcgtcaaagt ttctggacgg ttgcatgggt
    36721 tattcctgtt ttttgcaatg agagtcagtg cggcgttacg tatcaaacca acatcagccg
    36781 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    36841 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    36901 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    36961 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    37021 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    37081 tgccatcctt gattagattg atgaagccca ctttcttacc gtggcggagg attttcttgc
    37141 gcatcagttc tggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    37201 gctccgggta gccgctgaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    37261 tgcgcatatc gcgcagcggg ccactctcaa gatgctgacg atattccgcc agtttttgct
    37321 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    37381 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    37441 cgccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    37501 gcactacgtg agcggaatat tccaccagct tgccacccgc gatcagcggt gcaacggccg
    37561 gatgttgttt gaaatcttcc agcatttgcg gcaccgattt ttttgcgtca tgcagatgat
    37621 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    37681 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    37741 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    37801 ccacatccgt cggtttaacg cgttttgcca tccccagctt ttcggcaagg atggagttca
    37861 ctccatcagc aaggatcacc gttttcgctt caatcacatc gccatcggct tctacaccga
    37921 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    37981 ccgcttcttc ggcctgctcc atcagccagg catcaaattt actgcgcaaa acggagtaag
    38041 aacgctggga tggcgaggtt tcgtcaccat tgcagtagtc catagtcatc gctgactttt
    38101 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gagtcggcga
    38161 aaccaggaat aatgtgttcc aggctgtggg catagagacg cccgccggtg acgttcttgg
    38221 cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    38281 gtgcggcaac cgaaccggca agcccagcac cgacgatgat ggcgtcaaag atatcttcgg
    38341 acataagagc tccctgtcag cggcagggcc aaaatgcagc cctgccagag tggatcaacg
    38401 cgctaaagct gcggtcagcg ccggaaggat cttcacggcg tcaccaacaa tgccgtaatc
    38461 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgatgc
    38521 attagcgcca accatgtgct ggatctgccc ggagatcccc accgccaggt acagttcagg
    38581 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    38641 cgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    38701 cgcaatgttc tctttgctgc caataccgcg accgacgctg accaccagac gggctttgtc
    38761 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    38821 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    38881 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    38941 cgccagacca ccgtaaacca tgtgtttcac tgtcgcttta ccgtcctgta cgctgacggt
    39001 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    39061 gccgcgacgg gtgtttggca gcagcaccag gccgtctgcg ccgtgctggc gaatagtgtc
    39121 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    39181 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggt
    39241 gttgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagaaggggt
    39301 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    39361 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    39421 cgctgacgtt cgcgctgttt cggcgcggca acctgttgtt ctgaccaggc tgcctctgcg
    39481 ttaaaaccaa tatccgccgc cgaccatacc tggacgggct ttttcgccgc gccgagaatg
    39541 gctttcatcg aaggaatttg tggggaattg atatcagtgg aaacagcaac aaccgcaggc
    39601 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    39661 gcagtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    39721 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaaa
    39781 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    39841 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    39901 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggccgt cacctgcgcc
    39961 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttcagatc gtattggctt
    40021 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    40081 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    40141 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    40201 ataaaacttt aatcaccaat attgaaaatg tcacacgcag ataaatccaa ctttcaatat
    40261 tgttaagttc ctcaccaata ttgaaaacat ggcgtagcaa aaagaaattt tcaatattgc
    40321 tttatggatc accaatattg aaagtcagat gctatttttt tactcacaac acaacataac
    40381 aaactgatta ataatcaaaa ttaacgagaa aagcgccctc tattcacccg aaacaaaaat
    40441 gtgataacaa tcacagaatg cagcttattg aatacccatt atgagttagc
//
LOCUS       DAJOWB010000030        39717 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJOWB010000030 DAJOWB010000000
VERSION     DAJOWB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.030"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..1350)
                     /locus_tag="OHO85_001759"
     CDS             complement(<1..1350)
                     /locus_tag="OHO85_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCQ2671141.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRAPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     "
     gene            complement(1476..1781)
                     /locus_tag="OHO85_001760"
     CDS             complement(1476..1781)
                     /locus_tag="OHO85_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCQ2671142.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(1889..1965)
                     /locus_tag="OHO85_001761"
     tRNA            complement(1889..1965)
                     /locus_tag="OHO85_001761"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1929..1931),aa:Val,seq:gac)
     gene            complement(1970..2046)
                     /locus_tag="OHO85_001762"
     tRNA            complement(1970..2046)
                     /locus_tag="OHO85_001762"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2010..2012),aa:Val,seq:gac)
     gene            2354..3610
                     /locus_tag="OHO85_001763"
     CDS             2354..3610
                     /locus_tag="OHO85_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCQ2671143.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(3651..5024)
                     /gene="mdtK"
                     /locus_tag="OHO85_001764"
     CDS             complement(3651..5024)
                     /gene="mdtK"
                     /locus_tag="OHO85_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCQ2671144.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            5239..5880
                     /gene="ribE"
                     /locus_tag="OHO85_001765"
     CDS             5239..5880
                     /gene="ribE"
                     /locus_tag="OHO85_001765"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCQ2671145.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(5920..7068)
                     /gene="cfa"
                     /locus_tag="OHO85_001766"
     CDS             complement(5920..7068)
                     /gene="cfa"
                     /locus_tag="OHO85_001766"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCQ2671146.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(7359..8570)
                     /locus_tag="OHO85_001767"
     CDS             complement(7359..8570)
                     /locus_tag="OHO85_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCQ2671147.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            8683..9615
                     /locus_tag="OHO85_001768"
     CDS             8683..9615
                     /locus_tag="OHO85_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2671148.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(9612..10637)
                     /gene="purR"
                     /locus_tag="OHO85_001769"
     CDS             complement(9612..10637)
                     /gene="purR"
                     /locus_tag="OHO85_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCQ2671149.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            10936..11025
                     /gene="ynhF"
                     /locus_tag="OHO85_001770"
     CDS             10936..11025
                     /gene="ynhF"
                     /locus_tag="OHO85_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCQ2671150.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            11191..12360
                     /locus_tag="OHO85_001771"
     CDS             11191..12360
                     /locus_tag="OHO85_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2671151.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(12595..13176)
                     /gene="sodB"
                     /locus_tag="OHO85_001772"
     CDS             complement(12595..13176)
                     /gene="sodB"
                     /locus_tag="OHO85_001772"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCQ2671152.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(13304..14119)
                     /gene="mepH"
                     /locus_tag="OHO85_001773"
     CDS             complement(13304..14119)
                     /gene="mepH"
                     /locus_tag="OHO85_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCQ2671153.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTFADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            14453..14800
                     /gene="grxD"
                     /locus_tag="OHO85_001774"
     CDS             14453..14800
                     /gene="grxD"
                     /locus_tag="OHO85_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCQ2671154.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(14851..19467)
                     /locus_tag="OHO85_001775"
     CDS             complement(14851..19467)
                     /locus_tag="OHO85_001775"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCQ2671155.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEKA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIEEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPRVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(19560..20207)
                     /gene="rnt"
                     /locus_tag="OHO85_001776"
     CDS             complement(19560..20207)
                     /gene="rnt"
                     /locus_tag="OHO85_001776"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCQ2671156.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPTAEEV"
     gene            complement(20310..20717)
                     /gene="gloA"
                     /locus_tag="OHO85_001777"
     CDS             complement(20310..20717)
                     /gene="gloA"
                     /locus_tag="OHO85_001777"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCQ2671157.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(20798..21895)
                     /gene="nemA"
                     /locus_tag="OHO85_001778"
     CDS             complement(20798..21895)
                     /gene="nemA"
                     /locus_tag="OHO85_001778"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCQ2671158.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(21932..22531)
                     /gene="nemR"
                     /locus_tag="OHO85_001779"
     CDS             complement(21932..22531)
                     /gene="nemR"
                     /locus_tag="OHO85_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCQ2671159.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            22634..22873
                     /locus_tag="OHO85_001780"
     CDS             22634..22873
                     /locus_tag="OHO85_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCQ2671160.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            22922..23818
                     /locus_tag="OHO85_001781"
     CDS             22922..23818
                     /locus_tag="OHO85_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCQ2671161.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSTEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            23899..24420
                     /gene="sodC"
                     /locus_tag="OHO85_001782"
     CDS             23899..24420
                     /gene="sodC"
                     /locus_tag="OHO85_001782"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCQ2671162.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(24421..26433)
                     /locus_tag="OHO85_001783"
     CDS             complement(24421..26433)
                     /locus_tag="OHO85_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCQ2671163.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDVYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHTRLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(26433..27290)
                     /locus_tag="OHO85_001784"
     CDS             complement(26433..27290)
                     /locus_tag="OHO85_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCQ2671164.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVKAGDLLLTIDKTPFQIAELNAQAQLAKAQSDHAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(27293..27529)
                     /locus_tag="OHO85_001785"
     CDS             complement(27293..27529)
                     /locus_tag="OHO85_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCQ2671165.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            27730..28164
                     /gene="slyA"
                     /locus_tag="OHO85_001786"
     CDS             27730..28164
                     /gene="slyA"
                     /locus_tag="OHO85_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCQ2671166.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(28211..28678)
                     /gene="slyB"
                     /locus_tag="OHO85_001787"
     CDS             complement(28211..28678)
                     /gene="slyB"
                     /locus_tag="OHO85_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCQ2671167.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            28952..30061
                     /gene="anmK"
                     /locus_tag="OHO85_001788"
     CDS             28952..30061
                     /gene="anmK"
                     /locus_tag="OHO85_001788"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCQ2671168.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            30159..30488
                     /gene="mliC"
                     /locus_tag="OHO85_001789"
     CDS             30159..30488
                     /gene="mliC"
                     /locus_tag="OHO85_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCQ2671169.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            30547..31203
                     /gene="pdxH"
                     /locus_tag="OHO85_001790"
     CDS             30547..31203
                     /gene="pdxH"
                     /locus_tag="OHO85_001790"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCQ2671170.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            31332..32606
                     /gene="tyrS"
                     /locus_tag="OHO85_001791"
     CDS             31332..32606
                     /gene="tyrS"
                     /locus_tag="OHO85_001791"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCQ2671171.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            32665..33528
                     /gene="pdxY"
                     /locus_tag="OHO85_001792"
     CDS             32665..33528
                     /gene="pdxY"
                     /locus_tag="OHO85_001792"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCQ2671172.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(33572..34177)
                     /gene="gstA"
                     /locus_tag="OHO85_001793"
     CDS             complement(33572..34177)
                     /gene="gstA"
                     /locus_tag="OHO85_001793"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCQ2671173.1"
                     /translation="MKLFYIPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRLDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGFEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(34283..35785)
                     /gene="dtpA"
                     /locus_tag="OHO85_001794"
     CDS             complement(34283..35785)
                     /gene="dtpA"
                     /locus_tag="OHO85_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCQ2671174.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(36396..37031)
                     /gene="nth"
                     /locus_tag="OHO85_001795"
     CDS             complement(36396..37031)
                     /gene="nth"
                     /locus_tag="OHO85_001795"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCQ2671175.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(37031..37726)
                     /gene="rsxE"
                     /locus_tag="OHO85_001796"
     CDS             complement(37031..37726)
                     /gene="rsxE"
                     /locus_tag="OHO85_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCQ2671176.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(37730..38350)
                     /gene="rsxG"
                     /locus_tag="OHO85_001797"
     CDS             complement(37730..38350)
                     /gene="rsxG"
                     /locus_tag="OHO85_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCQ2671177.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(38354..39412)
                     /gene="rsxD"
                     /locus_tag="OHO85_001798"
     CDS             complement(38354..39412)
                     /gene="rsxD"
                     /locus_tag="OHO85_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCQ2671178.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(39413..>39717)
                     /locus_tag="OHO85_001799"
     CDS             complement(39413..>39717)
                     /locus_tag="OHO85_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2671179.1"
                     /translation="NAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRK
                     AAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAAAAAIARAQAKKAAQQKVVNED"
BASE COUNT         9993 a         9972 c        10311 g         9441 t
ORIGIN      
        1 cactttaagc ggacgctgtc cgcgggcatc aataaaaacg tcaaacgagt agctgttgtc
       61 ttccgttttc aggacggtgc gcgactcatt aatttccatt ttgtagtctt caccgagggc
      121 gagaatatga atgattcccg cttcacgtaa ggcaagtagg cgacgaatag actctgaagg
      181 gattgccgca taattatcga tgaaaaccct ggcaaggcct ttactgaacc gtttatggtc
      241 gtgttcattc agatgaggaa caatttcctg tacggcttca tgcaggcgca gaatgacata
      301 acgccaggga acagtatgtt tttctcgttt attgcgttcg acttcctgga gatttttttc
      361 tgcccagtca aatggggcgc gttgtttgcg ctcggcaaac caggcttgag caaaggaatc
      421 gacattcagg ctctctaagg ctatgcgttg gctccagtct ggatcagcaa acttgatttc
      481 ctctactatc aatctaaata cccgatccaa aaggccctct tcaccttttt gaatctcagc
      541 atttaatgcc tgatcggtga cgatgtgtaa gggctcgtag ggaatagggc aatagaaatc
      601 ggcttcgggt aaaatacccg tgcgcgacat caacgtaata tttagctttt cacttgcgtt
      661 atcgcggtta aaaacgacgt gttgtttatc atcttcaatg aacgaaccat gctgaatagc
      721 cactgccatt gccgcatcca gtccgctcaa ggatgttccc ataataccca cgttacacgc
      781 atcgaccttt gcttccatca ggcctgacca cggactggga aaatacgttc tggttgcttc
      841 ttcttcatca ggccagacgt gacccgtggc aatcaccgct aaatcaaacg tctcgctggg
      901 taaatcctga tttgtagcga gcatgacgcc agcatttgta atttgcagat cggtaacctg
      961 gcatgattca taaacagcca ctgcaaattt ttgctgtcgt gcctggtcta ctaatcgtaa
     1021 aaattgatcg cggaaatatt cgcccagcag aattcgcggt aaaaactgac gatcgtgcaa
     1081 ggtttctttt ttaacgccat aacgctggag atggctggct tcttgctttt gtagccattc
     1141 gagatacgta caattaatcg gcggtatttc aatactggca atatttgcca gcatcatttt
     1201 tgagttttcc tcatcactgt atggcatccc gacaccggcc tcgtcagcct gctcgaagat
     1261 agaaatagaa agtggagttt gttgctgtag aagcgagaat aaggtgtaga tccccgtagg
     1321 cccggcaccc acaatagcaa tttttttcat tgttacttac ctgtacgcat tctttcgaat
     1381 ttagtgttat cagataagtt tggttgagat ttttagaatt tgagaaattt cagacaggga
     1441 tgaaaagggc gctaaccgcc cttgattaaa ttctgtcagg cgagttttgc ctgattgatt
     1501 tgactcagtg gctcattgac atcgaaaact ttggcgacaa cttcctcaac gccgagattt
     1561 ttaagtcggg cagtatgttt ttccagatag gcaagggcgc ttttttcatc agtaaaaaga
     1621 tagatcccac cggcttcgtg gttcttttca ctttctgtcc ataccttcca cagaaaacca
     1681 ggttcctgat taatcgactc agcaagcggt ttaagctgct cagccattgc gtcgccaaat
     1741 gggccattga aagcaaaatg aagttgtaac aaagttgcca tagcgattct ccttctgata
     1801 acgcatacga agacagtgta aatcttttaa cgtattggat acagagaaga aacagaggga
     1861 aaaaggagaa gaccagatta agaaaatctg gtgcgtccga gtggactcga accaccgacc
     1921 cccaccatgt caaggtggtg ctctaaccaa ctgagctacg gacgcaggat ggtgcgttca
     1981 attggactcg aaccaacgac ccccaccatg tcaaggtggt gctctaacca actgagctat
     2041 gaacgcaacg ttgtaggtga caacggggac gaatattagc ggcagagtgg gaaggtggca
     2101 agaggcaaaa cgtaattttc tgcgctattt cgaccgtttg cagagctttt aagcaaattg
     2161 gctatatttt gttgatttgc aagggcgatt tttattcagg atcgcattta catctgatac
     2221 aacccaataa ttcgtgtatc agagtttaaa cagagttgtt tgccaccatc tcaaatctgt
     2281 cgtggataaa tcctccgtag cctttacact taggtagcaa taaaggcaca cagatgcagc
     2341 aaaggaataa atcatgggat ctgatgcgaa aaacttgatg agcgacggga atgtgcaaat
     2401 tgttaagacc ggcgaggtca ttggcgcgac gcaacttact gaaggcgagt tgattgttga
     2461 agctggcgga agagccgaaa ataccgtggt cacgggggct ggctggttga aagtggcaac
     2521 cggtgggatc gccaaatgca cacagtacgg taacaatggc acgctatcgg tcagcgacgg
     2581 tgccattgcc acagatattg ttcagtccga gggaggcgca attagtctct ctacgctcgc
     2641 tacggttaat ggccgccatc ccgaaggtga attcagcgtt gatcagggtt atgcctgcgg
     2701 tttgttgctg gaaaatggcg gtaacctgcg tgtactggaa ggacatcgcg cggaaaaaat
     2761 cattctcgat caagagggcg gcctgttggt taatgggaca acctcagcgg tcgtggtaga
     2821 tgaaggtggt gaattgttgg tgtatccagg tggggaagcc agcaattgtg agattaatca
     2881 gggcggcgtt tttatgctgg ccgggaaagc cagtgatacg ttgcttgctg gtggcaccat
     2941 gaataatctc ggtggtgaag actctgacac tattgttgag aatggatcca tttatcgtct
     3001 ggggacggat ggccttcagc tctacagttc cggtaagacg caaaacctgt ccgtgaatgt
     3061 gggtggtcgg gctgaagtgc atgccggtac gctggaaaat gcggtaatac aaggtggaac
     3121 agtgatcctg ttgtcaccca ccagcgcgga cgaaaatttt gtcgtagagg aagatcgcgc
     3181 accggttgaa ctgaccggga gtgttgcatt actggacggc gcttcaatga ttattggcta
     3241 tggcgcagat ctgcaacaat caacgattac tgtacagcag ggcggtgtgt tgattctcga
     3301 cggcagtacg gtaaaaggtg acggtgtcac ttttattgtt ggtaacatca atctgaatgg
     3361 cggaaaactg tggctgatca ctggtgcggc aacgcatgtg caactgaaag tgaaacgcct
     3421 gcgcggagag ggagcgattt gcctgcaaac cagtgcgaaa gaaatctcac ctgacttcat
     3481 caatgtgaaa ggggaagtta ccggggatat acacgttgag ataacagatg ccagtcggca
     3541 aactctgtgc aacgctctga aattacagcc agacgaagac gggattggcg caacgctcca
     3601 gcctgcgtaa aaacacaggc gcaacccgtg aaggttgcgc cttgtcttta ttagcgggat
     3661 gctcgttgca gaatgataac tgacggcaga cgttgcagga accgcatacg cagcatcatc
     3721 ataatggctg ccgacgtcag gccaataata aagcctatcc agaagcctgc tggccccata
     3781 ggttcaacga ccagatcggt cagtgccaga atatagccgc ttggcaagcc cagcacccag
     3841 taagccgtaa aggtgatata gaaaatggaa cgcgtatctt tataaccacg caaaatccca
     3901 ctgccaatca cctggattga gtcagaaatc tgatataccg ccgccagcaa catcaaatgc
     3961 gcagccagcg ttacaacctc gggattgtcg ttgtacaaca gggcgatttg ctcccgcagt
     4021 gaaaccgtga atatggccgt cagggttgcc atacagacac ccaccataag cccggtccgc
     4081 gcagcggttt gcgcatccag cgttgagccc tgacccagac gataacctac gcggatagtt
     4141 accgctgccg ccagcgacat tggaagcaca aacatcagtg aactaaagtt cagggcaatc
     4201 tggtgtcctg cgacatcaac aataccgagc ggagacacta acagagccac gacggcaaac
     4261 agtgtcactt caaagaatag tgccagcgca atcggcaaac cgagttgaat cagtcgtttc
     4321 ataaccgcag gatcgggttt cgcggtgcct ttttcgttac gaatatcgcg catggagcgg
     4381 gcgcgtttaa tgtaagaaac catggcaagg aacatgaccc aatacaccgc cgcagtagcc
     4441 acgccacaac caacgccacc gagctcaggc ataccgaaat gaccataaat aaagatatag
     4501 ttcaccggaa tgttcaccag caggccgata aagcccatta ccatacccgg cttggttttt
     4561 gccagacctt cacattggtt acgggcaacc tggaagaaca gatagcccgg cgcgccccac
     4621 aacaacgcac gcagataacc cacggctttg tccgccagag ccggatcgat gttttccatg
     4681 gagcggataa tgtaacctgc attccacagc acaagcataa tgagaacgga aacaaaacct
     4741 gccagccaga aaccttgtcg cacctgatgc gcaatgcgct cacgtcgacc ggaaccattt
     4801 aattgcgcga taaccggcgt taatgccagc agcagtccgt gaccaaagag gatcgccgga
     4861 agccagatag aagtaccgat agcgaccgcc gccatgtcgg tggcactata gccgcccgcc
     4921 atcacggtat cgacaaaacc catcgcagtt tgggcgattt gcgcgagaat caccgggatt
     4981 gctaatgcta ataacagacg cgcttcactg atatacttct gcacgtgaac accttttatt
     5041 tgtagttata tgagagacta aaaagccgcc tgaatgggca gcaaaaagaa gaagcagggg
     5101 aaattcagtc tattgtagcg aggatttatt aattctccag tgaaaaaata gtcattctta
     5161 ccccttcact ggtaagggcg tttgccacct gctattgtgc tgaacagaat gtcttaactg
     5221 atttcaggag ttgtaagtat gtttacgggg attgtacagg gcaccgcaaa actggtgtcg
     5281 attgacgaga aaccaaattt tcgtacgcat gtggtggagt tacccgacca catgctggac
     5341 ggcctggaaa caggtgcttc cgtggcgcat aacggttgct gcctgaccgt gacggaaatt
     5401 aacggcaacc atgtcagttt tgacctgatg aaagaaacgt tacgcattac caatcttggc
     5461 gatttaaaag tgggggattg ggtaaacgtt gagcgtgcgg cgaaattcag tgatgaaatt
     5521 ggcggacact taatgtcagg tcatattatg accaccgctg aagtggcgaa aatattaacc
     5581 tcggaaaata atcgccagat ctggtttaaa gtccaggata gtcagttgat gaaatatatc
     5641 ctgtacaaag gatttattgg catcgacggt attagcctga ccgtcggcga agtcacgcca
     5701 acgcgttttt gcgtccattt aattccggaa actctggaac gcacgactct tgggaagaaa
     5761 aaactcggcg cacgcgtcaa cattgaaatt gatccacaaa ctcaggcagt ggtagatacg
     5821 gtagaacgtg tgctggcggc acgagaaaat gccatgaatc aaccaggtac agaagcctga
     5881 tctgaaatcg cccccggaga gggggcgata gaatagcctt tagcgagcga ctcgaaggcc
     5941 gttttcaaca ccgcgtgaga acacgacctg ccagagctga atatcacggg cgcggaaagc
     6001 acctgcacag gcattcagat aataggtaaa cattcgttta aagcgttcac tgtagttatc
     6061 cgcaatttct ggccatgcgg cgaggaatcg ttcataccac gccatcaacg tagtatcgta
     6121 atcagcaccg aagttatgcc agtcttccat cacgaagtgg ggttcgctgg attgagcaat
     6181 ctggcgtaca gagggcaggc aaccgttcgg aaaaatatat ttattaatcc agggatcaac
     6241 attcagatcg gtttttttcg aaccgatagt atggagcagg aatatacctt ccggtttcaa
     6301 attacgatcc accaccgcaa aataggtatc gtaatttttc ggtccgacgt gctcgaacat
     6361 ccccacagaa acaatacgat caaactggtc gttcaggtca cgataatctt gcagcaaaat
     6421 ggtgacatcc aggccttcac agcgttcctg agccattttt tgctgttcgg cagaaatggt
     6481 gacgccgacc acgcttacgt catagttaga tgccatgtag tgcgccagtc cgccccagcc
     6541 gcagccaata tccagtacgc gcatccctgg ttttaactgc aatttttcac aaatcatttt
     6601 gagcttcgcc tgctgggcag attccagatt atcggcatct ttccagtaag cgcaggaata
     6661 ttgcatgaag ggatcaagca tgcggctgaa caagtcatta cccaaatcgt aatgctcctt
     6721 gccgactatc caggcacgtt ttttactctg cagattgaag agacgagcgc tggcaatacg
     6781 cagcgtgtct ttgaaatgat gggggagttg gttctcgaga cctgcgcgta agactttgct
     6841 aaaaaacata tccagtcggt cacattccca ccagccatcc atataacttt cgcctaaccc
     6901 caaagagcct tcttgcagaa cgcgtttaaa aaaatcgggg tttttcacac gaatatccgc
     6961 cggggcagaa ccgttaatgg ctataccggc acggctaagt aattcgttgg cgatacggta
     7021 ccagttgtca tccggtacac tgacttcttc tatacacgat gaactcatag tttctccatc
     7081 actggtcggt gatcagaacc ttaaaacagc gtagacgctt tttgggcttt gtgagaaatc
     7141 tcgcagaaaa aaccgcgagt ctataatccg acgtagaaca gagggaaagg aggaaccttt
     7201 gccgaaatga ccatccatag tgagtcggga acgatcctgt ccccgtaaaa acataatatt
     7261 tatcgtagct ttaacaactc gaaattagta taggccgcta atttcaatca tacaacaaaa
     7321 ttttgttagc agcctaagta taagtatatc gatatagatc agtgtgattc gctatgagcg
     7381 acttcggcat tgccatgatt ctggcagcca acttcttcac aacgttgcat catgtaaccc
     7441 agagcgacca gcactactgt tgataacatc acgctggtgg tggtgagcaa tggcgtgctg
     7501 atactgatca gccaggaaac taccagactt gcgaggaagc acagacccag ttgtagagtg
     7561 ttctgcaacg ctgcggcgcg accagttgcg tgtgggaagg gacgcagcgc ctgggcgaca
     7621 acaatcgggt agatcgcgcc attggcaatc gccatcacac agaatgggat caggatttcg
     7681 accagcgaca catggctaat gaagcctgca gcccaggtcg caatgacgct gactgcaaac
     7741 agcaccagca accacggtaa taactgcttg ccttgccatt tctgcagcgc ggcgcgacag
     7801 ccataaccac caatcagaaa cgcgatagtt tgcgggacat aacttaaacc aataactgcc
     7861 gggctgtagc ccatttcact aaggatgaac ggtgaaccgg tcagccatgc aaaaaaactg
     7921 gctgaacagg ccgcgtatat cagcacgttg ccgcgatagg ttttagaacg tagcaggtcg
     7981 gtaaaggtca gaccatcctg actattgtta cgggccttcg tcgtgggttt gagccagaaa
     8041 ataggcagaa tcagcaccac ggtaatggca aacagggtgg cgaaaatcgc ctgccaggaa
     8101 aaatggacca gcagccagct tcctaacaga ggagccaggg ccggagatag acccaccagc
     8161 ggcatgatgg tcgcaaaaat tcggttaact ttctgtgaag gataataatc cgtcactaac
     8221 gcttgccaga taaccgccgc ggcgcagaca cccacagcct gtacaaaacg caataccagc
     8281 agcgtagcgg cgttttctac ccacagcatc cccagactac ctaacgcaaa aattgtcagg
     8341 ccgattaata acaccggttt acgaccataa cggtcggaga gtggccccca cagaagctgg
     8401 gctgcggcaa aaccggcaag gaacagacta aggctggcac tgacagcaga cgcaggcgtt
     8461 tgcaggtcgg cctgtatggc ggcgaaagca ggcagataca tatcggttgc cagaaaaccg
     8521 agtacgctca aacccgccag ccagactaaa aatcttttcc caggttgcat gtatattctc
     8581 taaatctctc aattaaacac cgccgcaaag tgtagggagt gaaattagtg ttgtgaaacg
     8641 gtaatatttg atgactggtt tcaaaaattt tgcaggcaga ctatgtggtc agaatattca
     8701 ctcgaagttg ttgatgcggt agcgcgtaat ggtagtttta gcgctgcggc acaggagctg
     8761 catcgcgttc cttctgcggt cagctatacc gtgcgtcagc tggaagagtg gctggcggtg
     8821 ccgctctttg aacgacggca ccgtgatgtg gagctgaccg ctgctggagc gtggtttctc
     8881 aaagaagggc gctctgttgt caaaaaaatg cagatcaccc gccagcaatg tcagcagata
     8941 gcgaacggct ggcgcggtca gttagctatc gcagtggata atattgtcag gccagaacgt
     9001 acacggcaga tgatcgttga tttttatcgc cattttgatg atgtcgaact tcttgtcttt
     9061 caggaagtgt tcaacggtgt ctgggatgcg ctttccgacg ggcgcgtgga actggcgatt
     9121 ggcgcaacac gcgcgattcc ggtaggcggt cgttatgcct tccgggatat ggggatgcta
     9181 agctggagtt gcgttgttgc cagccaccac ccgctggcgt tgatggatgg cccgttcagc
     9241 gatgatacgt tgcgcaactg gccgtcgttg gtgcgcgaag acacctcgcg aacgctacct
     9301 aaacgtatta cctggttgct ggataaccaa aaaagagttg tcgtgcctga ctgggaatca
     9361 tcggcaacct gtatctcggc aggattatgc atagggatgg tgccaacaca tttcgccaaa
     9421 ccgtggctta atgaggggaa gtgggtggca ttagaactgg aaaacccctt tccggattcg
     9481 gcatgttgtc tgacatggca gcaaaatgat atgtcgcctg cgttaacctg gctgcttgag
     9541 tatctcggcg atagtgagac gctgaataag gagtggctgc gggagccgga agagactccc
     9601 gcaacgggtg attaacgacg atagtcgcgg aacgggccgt cagccacgga gcggcgttca
     9661 atcaagcgcg gatgcacttc aatggactgc ggttcttcac gtttgttgac gatacgatcc
     9721 aacagcatgt tgaacgctgt ttcacccagc gaatcttttg gctggtggat cgtggtcagc
     9781 gccggcgtaa aatagcgggc gttgcgcacg ttatcataac cgatcagcga aacatcctgc
     9841 gggacgcgca gacccatttc atcagcagca caaagtgcgc ccattgccat gatatcgcca
     9901 ccacagaaga cggcagtagg gcgatgcggc tgcgacagta tttgctgcat ggcgcgataa
     9961 ccagattcgg gttcaaagtc accctgaaca atccagcttt ccggcacctt gatcatcgct
    10021 tcttccatcg ccttcataaa accggcaagg cggcctgcgc cggtgttacg ttccagcggg
    10081 ccggggatga cgccgatttc gcggtgaccg cgttcaatca gataacgccc ggccatgtag
    10141 ccgccttcga acgcgttatc aatgaccgca tcggtgaagt cagcttttgc ttcaccccag
    10201 tccatcacca ccattgggat atggcgatac tcttccagca tcgccagcaa cggctctggg
    10261 tactcagaac acatcaccag cagaccatcg acgcgttttt gcgccatcat cgacagataa
    10321 gcccgctgtt tctcaagatt gttccacgca ttacccagaa tcagggtgta acctttctgg
    10381 aagcaatttt tttcaaccgc ttcaatgatc tcggcaaaat aggccgcttc gctgctggtc
    10441 gccagcaaac cgatagactt ggtgtggtta accttcaggc tacgcgccac cgcgctaggg
    10501 gagtagtgta attctttaat cgctgcccac acggcgttgc gcgtttcttc agcgacgaaa
    10561 cgtgttttgt tgatcacgtg tgacacagtt gtagtggaaa cgtttgctcg tttcgctaca
    10621 tcttttattg ttgccattcc atttcactcc agaccctaac ttcagctcct gcaaaatcgc
    10681 aaggtaaacg tttgccttta cacaccttac gcacaacaaa agtgttgcgg tacgccggaa
    10741 aaacgacacg gagcgtcagg aaggggtcaa tggccagtac gcaaatagtg taagcgtgga
    10801 attttggccg atcttgacga aaaggggaag tggtaaaaac acttatcatt ataaatgacg
    10861 ggagattttt tcatcacagt gtgtaaaaat gcgtaatatc actttttgtg tggtaataaa
    10921 aaaggagaaa acttgatgag caccgacctt aaattttcac tggtaacaac gattatcgtc
    10981 ctcggtttga tcgtagccgt gggtttgact gccgcgctgc actgatttcc tctcgaggga
    11041 gcgcgttctc cctcgcatct ccttcctgtt tgttagcaaa atggcaaaag tgttttctct
    11101 ttttgttatt ttcatttttt tgtgattgct gtcatgcttt cgccacttta atgaatatcg
    11161 cgtaatgacg cgaagaggtg gagataaggc atgaaaatta actatccgtt gctggcgctg
    11221 gcgattggcg cgtttggtat cgggacaacg gagttctcgc caatgggctt gttgcccgtc
    11281 attgcgcgcg gtgtggatgt ctcgattccc gctgccggaa tgttaatcag tgcctatgca
    11341 gttggcgtaa tggtgggcgc gccgctgatg acgcttctac tttctcatcg tgcccgccgc
    11401 agtgcgttga ttttcctgat ggcaattttc acgctaggca acgttctttc cgccatcgcg
    11461 ccggattata tgaccctgat gctttcacgc attttgacca gcctgaatca cggagcattt
    11521 tttggtttgg gttcagtcgt ggccgcaagc gtggtgccaa aacataaaca ggccagcgca
    11581 gttgccacta tgtttatggg gttaaccctg gcaaatatcg gtggcgtgcc ggcggcgacc
    11641 tggttgggtg aaaccatcgg ctggcggatg tcatttctgg caacggcggg gctgggagtg
    11701 atttcaatgg taagtctgtt cttctcatta cctaaaggtg gtgcaggggc acgacctgaa
    11761 gtgaaaaaag agctggcggt attaatgcgt ccgcaggtgc tgtctgcatt gctgacgacg
    11821 gtactgggag ctggtgcaat gtttactctc tacacctata tctctccggt actgcaaagt
    11881 attacccacg caacaccggt gttcgtcacg gcaatgctgg tgctgattgg tgtcggattc
    11941 tctatcggta actatctcgg cggcaaactg gcagatcgtt cagttaacgg cacgttgaaa
    12001 ggctttttgt tgctgctgat ggtgattatg ctggcaatcc cgttcctggc ccgcaatgag
    12061 ttcggcgcag ctattagcat ggtggtgtgg ggcgcagcaa cctttgcggt cgtaccgccg
    12121 ttacagatgc gcgtgatgcg tgtcgccagt gaagcgccgg gtctgtcttc atcagtcaat
    12181 attggtgcct ttaatcttgg aaatgcgctg ggagcagctg ctggtggtgc ggtaatttcc
    12241 gctgggctgg gatacagctt tgtgccggtg atgggagcga ttgtcgcggg actggcatta
    12301 ttgctggtgt ttatgtcagc cagaaaacaa cctgaaacag tttgcgttgc taacagctaa
    12361 ttaaacaaaa acgcagaagg ataaccttct gcgttacagt tgctgacaac gtgcgattta
    12421 tcttgccgga tgcggtgtta acgactcatc cgcgttgaca tgaaaagacg cgttaggcgt
    12481 cgtatcatgc agcgcttgtc ggatgcggcg agtgccttat ccgacctaca tcgatgatcc
    12541 gcgtaggcct gataagcgta gcgcttcagg caatgctgca tttgccatca gttattatgc
    12601 agcgagattt ttcgctacga attcccagtt caccagcgcc cagaagtgct ccagatagcc
    12661 aggacgtgca ttgcgatagt cgatgtaata agcgtgttcc cagacatcaa cggtcagcag
    12721 cggagtcgca tcggtggtca gcggagtacc cgcgttagag gttgaaacga tagccagttt
    12781 gccatcgctg tttttcacca gccaggtcca gccagaacca aagtttttga tcgctgcatc
    12841 agtaaactgc gctttgaaat cggcaaagct gccaaaagat gcggcgatag cttcagcgac
    12901 ttttccagtc ggttcgccac cggcgttcgg tgccaggcag ttccagtaga aagtatggtt
    12961 ccagacctga gctgcgttgt tgaatacgcc accttcagag ctgcgaataa tctcttccag
    13021 tgatttacct tcaaacgcgg tacctttaat caggttgttc aggttagtga cataagtctg
    13081 atggtgcttg ccgtagtgat actcgatggt ttccgcagaa atgtgcggtg ccagagcatc
    13141 tttagcatat ggtagtgcag gtaattcgaa tgacattgct actctccttt attattaatt
    13201 tgcatacgta caatagcctt attgtgcgta tggatagggt agcaaattaa aaggtgagac
    13261 aaaagatgaa cttaccctgt tgccgtaaca acagggtaaa gttttagcga agtgtttttg
    13321 gggtcattac ccgacgagcg ccaacatagt ggcgctgcca gtagtcttca ctgagagaag
    13381 tgatttgaat ttcctgacct gtgcgcggtg actgaataaa tttgccgttg ccgacataca
    13441 cgccgacatg atcggctgtg ccgcgtccct gagtacggaa aaagaccagg tcgccgtttt
    13501 tcagttcact acgttcgatt ggcgctgcat cacgcaggtg atacatttca ttcgccgtac
    13561 gcggaatacg aattttcacc aaatctttat aagcgtaata aaccaggccg ctgcaatcaa
    13621 aaccggtacg cggtgagctg ccaccccaac gatatggctt accaatttgc tgcatcagtt
    13681 tattcattgc cactttcgta gctttttgca ccttcgcttt gtgcgcgtcg gcaaaagttc
    13741 ctgacgcggc atttttgact ttcacacaat gcgatttata gcccttacgt ttggtacact
    13801 tttcggtaaa gctggctgag gccgttttat tgacggcgtg tttgctgcga gttttggcgt
    13861 ttttagaagc ggtaatggaa gattttttaa cggaagaagc ggttttgctt ttggtcgtac
    13921 ttttactggc ggcttttttc gctgttttct gggtcttttt ggtggttgcc gtgcttttct
    13981 ttttatctgc ttttttggtg atatgagtgg tagcagaact ctccctggct tgctttgaag
    14041 catgggccat aggcgttaaa ggcagggtgg taaaaagtaa agcacagagc gtgatcgaaa
    14101 tacggtttat ccgcgccact gagcgtccct ctgttaaggc aggtctatca tgcctgcgtc
    14161 tgatttacaa aacttgatga ttctaatgca tagaagaaga gaaagttaat agcttttatt
    14221 gttaaaaaat acgtttcgtt ggcgagcgca aaaaaatgca gcagtgaaag ctaaaaatgg
    14281 ttataaatac gccagtttgc cggcatcaga gaagaaacga atatttgaaa tgcaacaaat
    14341 agagcaaagg gataaaatag caaaagcgtg atgaaaatgt tattttccga tgccgcactg
    14401 acccgctaca atgtcagatt actgccgtaa agaagtttga ggaagcaaga caatgagcac
    14461 cactatcgaa aaaatccaac gccagattgc tgaaaacccg atcctgctgt acatgaaagg
    14521 ttcaccgaaa ctgccgagct gcggtttctc tgcccaggca gtccaggcgc ttgccgcatg
    14581 tggcgaacgt tttgcctatg ttgatattct gcagaatccg gacattcgtg cggaactgcc
    14641 gaaatatgct aactggccga ccttcccgca actgtgggtt gacggcgagc tggtcggcgg
    14701 ttgtgatatc gtgatcgaaa tgtatcagcg tggcgaactg cagcagctga tcaaagaaac
    14761 tgccgctaaa tacaagtctg aagagccgga cgcggaataa tccgtcctgc aatactgacg
    14821 gatgcgaacg ggcatccgtc agtcctttct ctatccccaa tccagccctt gtggcgaact
    14881 tgaaaatccc gcctcacgca gcagcgtaaa catcggcgtt tgccggaccg gtagatcatt
    14941 cacttctgtt agcgtaaagc gcagccgtgg ttcgcgacgc agtgcggtag tcagcgcgtg
    15001 gaaaacctct ggggcgagta attcctcttt ttcctgccag accagcattt ttttgccacc
    15061 ttgcgccaga tagagtaaca atttgccgcc agaaacgacc accagcgcac ccgcccgacg
    15121 cgttggaacc agcgttgccg gatgtgcagg ccagggcaga agatttcccc acacattagc
    15181 cggatcgttg gcagagagcg ccactggtgt atagtggcgc gtttgcgtgg cttgtgtcgc
    15241 cagatcgcgc aatcggtcaa tagtcagacg ttcagcgaat tgcgcgccac ccagaccttc
    15301 tacaaaacga cctcgcataa ttcgcccgga gtcttccata cttcgacaaa gcgtttgcat
    15361 cgatggaaac ccgccaggga tattttcggc tatcactgcc tgacgactga tgatgccgta
    15421 gcggtcgagc atattttccg ccagcgccag catcctttcg gtatcgttta gtggctccac
    15481 ctgcaataac gaccagcgtc cagccagatt tggtgtgttg taagataccc gcggcgagac
    15541 gggttgcgca tagacaggac gtccacgacg agcccggtga ctgcggcgag ttgaggtgcg
    15601 tgcgttggaa ctgctgcggg tgagggcgcg taacggtgcc caaatgtcgc tggtgatgac
    15661 gccttgccag accagcgccc ataacgcctc ttgcagggca gagagatcaa ccgattcgcc
    15721 gattttgtcg cgtatccgtt ggctgatttg ttgtgcaaac caggctcctc cgtcagccag
    15781 aacagcgact atcgcttgct gcagcgccga acgattcgcc tgatccgctt cggcgggagt
    15841 gaacgattct gcagcatatt cctgtagatg cagtgccacc aggccgtcat cttcacccag
    15901 ttttttttgc cccgaccaga taaccgcacc ggttgccagt aattcatcga gcatttctga
    15961 cgaatagtcg cgtacgcggg caggcaggat ctggctttcc cagagtgagg cgggtaaacc
    16021 gactccggca agctgttcga tcacccgcat cacgccatct acgccctcat aaacgcctgg
    16081 cgatgttgag gcaaagagcg ccgggctacc atcggtggcg ggtaatacgc cctgacgttc
    16141 cagcagcaat cgcgcatagg tcgtggctgc aacgggacgc gtcgcttctc tggcggcttg
    16201 cagcgagcgc aaacgcagac gacgaaatac ttcatcgctc acccagatgt cttgttgcag
    16261 attcatcacc agaccctgtt cacgcagttg ctgaagctgc tcttcgacaa tggcaatacc
    16321 gagactaaac tcatgagcca gttgttcagc cgtgaccaga gcatgagccc ggagatagcg
    16381 cagaaagagg tcgcgaagcg ggtaactgac tctatgtaaa taaatctctg gcaatgactc
    16441 tggtagtcgt acgccgaggg catcacgcag cctggcggca tcatccatac aggcaagacg
    16501 ctcctgtccg ctaatcatcg ctgggaagat tcgttttact gcaagaagat tttccagata
    16561 cgacgcaacc tcttcactgc tgcctgtatg ccgttgcgcc aggtcttcaa cggtcattgg
    16621 ccccagttcg cgcagcaggt cgaacaatcc ttcttcacct ttcgctcttc tgccaggagc
    16681 cagtcgttgc aactcttctt ccacctggcg aatgacctgc gggtcgagta attcccccgg
    16741 atcgacctgt ccgagtagat tgcgcagtaa ctcgctgtcc agcgacagta cggatgcccg
    16801 gcgctctgcc agcggggcgt cgctctggta cataaattcc gcgacatagc cgaacaataa
    16861 actggtggcg aaaggcgagg gcgtagtagt cgttacatcg gatatttgaa tttcgccacc
    16921 gttcaggcga cgcatcaaac gttccagtgc gggaagatca taaacatctt gcagacattc
    16981 gcgtagggtt tcgagaatga ccggaaaatc cggatatccc tgagcgattt ccagcaactg
    17041 actggcgcgc agccgttgtt gccaaagcgg ggtgcgatgg cccggcgtgc ggcccggcat
    17101 taataatgcc cgcgcggcgc attcgcgaaa acgggcggcg aaaagtgccg aactgcctac
    17161 cgcctcgcgg acaatttgca gcaacttttc tggttcaaac aaaaaaatcg cggcatcggg
    17221 caatttacca tcggtgtcag gaatacgtgc aacaatgccg tcatcactgg cgaccaccga
    17281 cgcgtcagcg ccccataggg catgtattcg cccggcaatc gccagcgccc agggttcatg
    17341 cacccgtctt ccatagggag agtgcaaaat aatacgccag tcaccaattt catcacggca
    17401 ccgttcgagg accagatggc gactccccgg aacgatgccc gtcgcgttgc gctgctcctc
    17461 aatcaacccc tgaatattgg cgatcgtatt ttcttctgcc agccacgggg gaatagtccc
    17521 ggaaaagaac gcgccatccg ccagcaaatg aagaaaatcg ccgatcatct cgcctaattc
    17581 agccggacgt ccgttacctt caccacgcca gaaggggagc cgggcagaac gacccggagc
    17641 aggagtcaca atcacctgat cgcgggtgat ttgctggatc cgccatgagg tagcgccgag
    17701 agtgataatg tcgttcaccc gcgactcata taccatctcc tcatccagtt cacccacccg
    17761 ccgcgaaccg gccttttctt caccttcggg taataacacg ctatacatgc cacgatccgg
    17821 aatggtgccg ccgctggtaa ccgccaacaa ttgagcgcca ggtcgggcgg tcaatatccc
    17881 ggtctccctg ttccagacca gtttggggcg aaaagcagaa aaatcgccag agggatagcg
    17941 cccggaaagc atatccagcg tggcgtcaaa aacacgtctt ggcagatctt tccacggtgc
    18001 ggcacggcgt acgcgggagt accattcgtc tacctgtaat gcatccatcg ccgcggcggc
    18061 aacggtttgc tgcgcaagga cgtcgagagg attatgcggt ggtgtcaggt tttccagcct
    18121 gcctgcgaac atacactcta caatgactgc ggaatcgact aaatcacgcc gggtacgggg
    18181 gaaaaacagc cctttagata caccgccaac ctgatgtccg gcgcgaccaa tgcgttgtaa
    18241 cccactggca acagaaagcg gcgttgccac ctgaatcacc agatccaccg cgcccatatc
    18301 aatccccagt tcaagactgg aggttgcgac cacgcagcgt aattcacccg atttcagcgc
    18361 ctgttcggtg attgctcgtt gttctttaga gacggagccg tggtgtgagc gggcaataaa
    18421 aacgtcgcta ctttgtacac ggttagaggt tgcgccggag gtcgactcga aatgggccgc
    18481 atcaacggcg atagacgggg aacgctgtaa gcgtgcggcg taaagctcat ttaatcgtgc
    18541 cgtcagtttt tccgccagcc cccgcgaatt agtaaagaca atggtcgagc gatggcgcaa
    18601 cacttcatca aggatgcccg tttcaatata tggccagatg gagccttccc ggccggcatg
    18661 gctgtcttcg ccagtgccgc tggcgaccga tgagacatca tccatattgg cgaccggtac
    18721 gacaatccgt atctgcggat gacgcattgc gggcgggttg actaccgtaa ccgggcgatc
    18781 gccaccaaga aatgctgcca catcgctggc tgagcgcaca gtggcagaaa ggccaattcg
    18841 ctgtgctgag gtgtggagca gcgcatcgag ccgctccaga cttaacgcca gatgcgcacc
    18901 acgtttactg cccgctaccg cgtggacttc atcaataatt accgtttcga cgccgcgtag
    18961 cgtttcgcgc gcgcgggagg tcagcatcag atagagtgat tcgggtgtgg tgatcagaat
    19021 atccggcgga ttacgggtga gtttgctgcg ttcctgtgca ggcgtatcgc cagtacggat
    19081 ccctacgcga agattgactt ccgtttcgcc gcgccgccgc cgttcatcgg caataccctt
    19141 caacgggatc tgcaagttgc gctgaacgtc ggtgcccagg gcttttatcg gtgaaatata
    19201 gaggatgcgt gaggttttac gcttatgcgc ctcgcgggta tcttcgccgc cttcgcggaa
    19261 gagccgatcg agggcgtaga gaaatgctgc cagcgttttc ccggagccag tcggtgcaat
    19321 caccagcgca tgttcgcctc gcgccgccac atgccaggtt tgcgactgga cagcggtcgg
    19381 ctgtttaaag gcgcgaagaa accagtcgcg ggtcgccggt gaaaacacgt ccggcaggag
    19441 cgatgaaggg tctggattat ctgccattaa cgctattccc cgtgaatatc atgcaggatg
    19501 cgtggaatag cctcactata gatgcctgaa tcattatatg tcatcaggca tcgactcgat
    19561 tacacctctt cggcggtggg tagcggccag cctcccagac gtttccagcg gttgacgatt
    19621 tcacaaaaca gcacagcagt gcgttcggtg tcgtacagcg cggagtgcgc ctgggtgctg
    19681 tcgaagtcca tgccagcggt ctggcaagcc tttgacaata cggtttgtcc gagtgccagc
    19741 ccggccagtg cagcagtgtc aaaagtggcg aaagggtgga acgggttacg tttcagcgag
    19801 gcgcgttctg cggcggccat cataaagctg tgatcaaaat tggcgttgtg cgccaccata
    19861 atggcgcggt tacagccgct cgctttaata cctttacgta caactttaaa aatttcgtgc
    19921 agcgcctcgt attcgctgac cgcgccgcga tcgggatcgt tcgggtcaat gccgttgaag
    19981 gcgagggctt ctggttgcaa atttgcgccg acaaatggtt cgacgtggaa atgtaatgtg
    20041 gtgtccggca tcagccagcc ttgttcatcc attttcaggg tgatggcggc aatctcaagc
    20101 agcgcatcgg ttttggcgtt aaatccggct gtttcaacat cgatcacaac aggataaaaa
    20161 ccacgaaaac ggtcgcacag accggtaagt tgagcgttat cggacatctg attctcttaa
    20221 tacggataaa ttgcagcgcg cattatgaca aatattactt gtcgatgcag taaagatgcg
    20281 ggcgcgatga gttcacgccc ggcaggagat tagttgccca gaccgcgacc ggcgtctttt
    20341 tcttcgatta actcaatttt gtaaccgtcc ggatcttcca caaacgcgat aaccgtagta
    20401 ccgcctttta ccggacccgc ttcacgggtc acgttacccc cgttttgacg gattttttcg
    20461 cacgcttcag cggcgttatc tacgctaagc gcgatgtgac cataagcagt gccgagttcg
    20521 tatttatcca cgccccagtt gtaggtcagt tcaatcaccg cttcttcggt ttccgggccg
    20581 tagccaacaa acgccagtga gtatttgtat tccgggtttt cgctggtacg cagcagtttc
    20641 atgcccagca ctttggtata aaaatcgatg gagcgttgca aatcgccaac gcgcagcatg
    20701 gtatgaagaa gacgcatctt tttatcctca aaatggctaa cagattcaaa atgttgtttt
    20761 agtatagcgg cggctttacg ccgctcgcaa tgttggatta caacgtcggg taatcggtat
    20821 agccttccgc gccgccaccg tagaaacttt cggcacgctg tgggttaagc tcagctttgc
    20881 gctgcaagcg ggcgaccaga tccgggttcg caatccagtc acgaccaaat gccaccgcat
    20941 caattaaccc tttgccgatc agcgtttcag ctttttctac tgtgtatgca cctgcgccga
    21001 taatcggacc gtggaaacgg gcgcgtactt tttcgcggaa cgcatcagta tacggttcac
    21061 cccccgccca atctggttct gacatatgca gataagcaat gccgcgttta cccagttgtt
    21121 caatcagata cagtgcatcg gcttcttcat tcgggccgtt atccgtgttc tggaaagtac
    21181 cgattggcga aatgcgaatg ccaatgcgat cggcacccca ttcttcaatc ccggcatcga
    21241 ccacttccag taccagacgt gcgcgatttt ccacgctgcc gccgtactga tcggtacgat
    21301 ggtttgaaga aggagaaagg aactgatgca gcaaataacc gtgagcagag tggagctcta
    21361 ccagatcaaa accggcttca cgcgcgttag caatggcctg acggaaatca ttgacgatac
    21421 ctggaatctc ttccagttca agcgcacgcg gcatggatgt ttcaacacgg atcgcctgac
    21481 cattttcatc gcgcagagaa gtacgtgttc ccgcgctaag tgctgaaggc gctaccggtg
    21541 cctgaccgcc aggttgcagg ctggcgtgag aaatgcgtcc ggtgtgccac agctgcacgg
    21601 ccatatgacc attttcagca tgaacgccag cggtgatttt tttccatgcg gcaatttgct
    21661 ccggactatg gatgccaggc gcacctgcat atccttttgc ctgggcagaa atttgcgtgg
    21721 cttcactaat aatcaaaccg gcactggcac gttggcgata gtattccgcc atcaacgggg
    21781 taggaatgtc acccggttca atactgcgca gacgcgtcag cggtgccata aaaatacggt
    21841 ttgccgccgt gatcgcgccc actttcagtg gggaatacag tttttcagat gacataaagg
    21901 ctcccggtcg tctggtgata aaaaatgcct gctaaacggc aggcgtcgca ataatgtttt
    21961 ttacatgggc cagcgcgttt tccagtggct cgaaactgcg cgaaattttg gcctgcagat
    22021 tcgcgccaag ccacagtgcg taaagcactt gtgcctgttg cagcggttcg ccacaaaagg
    22081 ttaaacaatg gttctcacgg ccattttcca gcgcctgaga gagcagggcg atcacgccgc
    22141 gagcaccttt atccatcgcg ctgcgcatat cttctgacag atcgcacact tcggcagaga
    22201 gttttactgt caggcaacca ctgatggttc catgttggca aaactggttc agtgtttgct
    22261 ggtaataagc cagtatgcgg tcgcggtagt taccttcgcc ggattgcagc aactcagtca
    22321 gtcgctggtg atatgcggcg taatgacgct caagcatggc aacgccaaac gcttctttag
    22381 agcgaaagta gtgatagaag gaccctttcg gcacttcagc ggtttttagt aattcgctta
    22441 gccccatccc ggtgaatcca cgttgcaggc aaagttgctc gcccgtcgcc aggagatgtt
    22501 cgcgagtatc atgttcggtg tgtttgttca tgcgttggag tgtagtagac cagtcggtct
    22561 agtgcaagca aagttgctac tttggacatt aacagcttca ataatggtaa taatcttatc
    22621 tgaggagggc gaagtggcgg agcaattaga gttctttccc gtccagagcc cgtgccgggg
    22681 aatttgccag tctgacgaac gcgggttttg tcgcggctgt tttcgtagtc gtgatgagcg
    22741 ttttaactgg aataaaatga gcgatggcga aaaacaagag gtgctgcgcc tttgtcggca
    22801 gcgattaatg cgcaaattac gcgcaaataa gccagcgtca tcagacgaac cggagcaacc
    22861 atcactcttt tgaacctaat cttgcgtata ctcctctcat atctgataag aggaagcgat
    22921 tatggttcag cgtattacta ttgcgccgca aggcccggag ttttcccgtt ttgtgatggg
    22981 ctactggcga ttgatggact ggaatatgtc cgcccgccag ctggtcagtt ttattgaaga
    23041 gcatctggat ctcggcgtga ccaccgtgga ccatgctgat atttatggtg gctatcagtg
    23101 cgaagcggcg tttggcgagg cactgaaact ggcacctcac ctgcgtgaac ggatggaaat
    23161 cgtcagtaaa tgcggtatcg cgacgaccgc gcgtgaagaa aacgtcattg gtcattacat
    23221 cactgaccgc gatcacatca ttaagagcac cgaacagtcg ctaattaatc tcgcgaccga
    23281 tcatctggat ctgctgttaa tccaccgacc agacccgtta atggatgccg atgaagtggc
    23341 ggacgcgttc aaacatctgc atcagagcgg caaagtgcgt cattttggcg tatcgaactt
    23401 tacgcctgcg caatttgccc tgttgcaatc acgtctgccg tttacccttg ccactaatca
    23461 ggtggaaata tccccggtgc atcagccgtt actgctggat ggcacgctcg accaactaca
    23521 acaactgcgt gttcgtccga tggcgtggtc ctgccttggt ggtggtcgtc tgtttaatga
    23581 tgattatttc cagccgctgc gtgatgaact ggctgtggtg gcagaggagt taaacgcggg
    23641 ctcgattgaa caggtggttt acgcctgggt attacgttta ccatcgcagc cgctgccaat
    23701 tatcggttca ggtaaaattg agcgcgtacg ggcagctgtc gaagcagaaa cactgaaaat
    23761 gacccgtcaa caatggtttc gtatccgtaa agcggcactg gggtacgacg taccgtaagc
    23821 acttttagga atagccgccg ttcaaaaatg tgtcactggt ttacacttat tcaggaatgc
    23881 acaatgaacg gaggtcctat gaaacgtttt agtctggcta ttctggcgct ggttgttgca
    23941 accggcgcac aagctgccag tgaaaaagtc gagatgaacc tcgtcacgtc gcaaggggta
    24001 gggcagtcaa ttggtagcgt caccattact gaaaccgata aaggtctgga gttttcgccc
    24061 gatctgaaag cattaccccc cggtgaacat ggcttccata ttcatgccaa aggaagctgc
    24121 cagccagcca ccaaagatgg caaagccagc gccgcggaat ccgcaggcgg gcatcttgat
    24181 ccacaaaata ccggtaaaca tgaagggcca gaaggtgccg ggcatttagg cgatctgcct
    24241 gcactggtcg tcaataatga cggcaaagct accgatgccg tcatcgcgcc tcgtctgaaa
    24301 tcactggatg aaatcaaaga caaagcgctg atggtccacg ttggcggcga taatatgtcc
    24361 gatcaaccta aaccgctggg cggtggcggt gaacgctatg cctgtggtgt aattaagtaa
    24421 ttaagaggcc agcgtacctt gcggtggtgc ttgctcaagt tgcgaaagcg agcagtacag
    24481 ccgccagaca attgccgcca gctcacgggc ggcaggttga tgatgacggg caaggctgtc
    24541 gcaaatccgc tgtaattctt caagtgtggc cgccagtgat ttttgctgaa cgccacgctc
    24601 actcatcact ccccgcaaca gtgaaataca gttatcccgt actcgcgata acggatcgga
    24661 acgcgattcc cagtcgcgca attgccagac aacatgagaa cagttcagca gcactacacc
    24721 ccagcgtaat aaccaacggc gagccagcgc atcctggctg ttactcaact gactgacgtg
    24781 atgataagtg agcgattcaa attcgctttc actcagtgtt ggatggcggc ttagctgatc
    24841 gacaaaatcc cggcgcagcg cgcgaatatg gcggcggctt ttacgagcat ccgatcctgg
    24901 acgcagaatg gcgaacgcta accacgccaa cgcgacgcca acgatttttg ccagattatc
    24961 gttaagaaaa tcagcaaaat catacaccgg gggattagtg acagcgataa aagaacccat
    25021 aaaaacaatc agttgccccc acaatgcggc aaattttggc atctgcaatt taagaagctg
    25081 cattgtcgcc agcagtggaa agagaaacag taaaaattgc cacagatcgc taatctggac
    25141 catcagacca aatttgacca caaagctgaa tagcgaaagt aacaccagcg tgcgcatcag
    25201 aagtgacaac gacttaaacg gtgctgcgac ggcggagtag agtacgcagc taattgctgc
    25261 cagcgttaat gcattggcac cggcatccca ttgcgaagca atactccatg cgccaatcat
    25321 catcaacgta caaaatgtac gcagcccgct ccacatagct tcggcgttat cggtatgacg
    25381 agccaggccg gatgtccgtg ggagtctggt atgatcatct acaccgcttt gcagacgatg
    25441 taattcctga ctactttgca gataaaggcg gcaaaaataa cgtagtcgct gccagaaggc
    25501 gacgtgccga tagtcggcga cgttggtcgg gcgtagcggg gcgataatac gtgcgacggt
    25561 gtaaacatct gtttgcgaac tggcgagcgc cgtcagcaac tgttcgagaa tttctcgtgt
    25621 ggcacctggc ggtgagggcc agttgagcaa catacgtcgc aggctagaga tgacactggt
    25681 catacgtaat tgctggtgga gcagcgcatt aaggcgcgcg ttttgctggc gaaaacgata
    25741 gtggctccag aaagcctgga tacgcagcaa attcatggtc agtatctgcc caatcacccc
    25801 ttcatgtgct gcacgaatgg catcggttgt ttcaggctgc cagagtaaac tggcatgttc
    25861 aagtagtcgg gcgtgcatgt ttttcaatgc ggttaaaagg gctgtagcat cggaactgct
    25921 aggcaggatc atcatcatca tgccgccgca caaaatgccg acaatcacct cgcaaacgcg
    25981 cgcctgagcg atatcccaca gctggctggc ctcagtaata ttaaccatcg gaaaggcgat
    26041 gatggcagcc gtgtagcccg ccagttgaaa tgcatacgcg acgttattcg tgaagtgcgc
    26101 acaggcccag gtacaaaagc caagccacgc cgacatgctc aatagaaaaa accacggctc
    26161 attgagcgta tgcccggcaa gaagcagtgc cgcaatggct ccgagcaaac tgccagcgat
    26221 gcgtccgagg cttttgctga taacaccgcc aacggtggga aagctaacca ctgcagccga
    26281 ggtcatcgcc caatagggtt catccagatt taaataatag gcaaccgtca gcgccagaca
    26341 catggcaatg gtattgcgta acgcataacg ccattgcgcc agcgtggccc tgaaccaggg
    26401 caaattgcgc aaggaccatg acgatgcgtt cattattgac ctacggcaat gctacaggtg
    26461 gtgcccgaca ccaacgtgac atcgcctggg actttatcca gcgcaaagcg gacgggaacg
    26521 cgttgggcga gtcgaaccca ggggacgtta ggtttaacat ccggaataag gcttgagtca
    26581 ctttcaacgc tctgatcata aatcgcccga ccgatactgg aaacgtgacc ctgtaacgtt
    26641 ttgttatcac tatagagggt aatttgtgct ggtgcacctt cgcggatatg gcgcaacttg
    26701 gtttcttcaa aataaccaat gacgtaaaac gagtggctgt cgacaagggc aaacagcggt
    26761 ttcccggtgt cagcgtaatc gccgatgcgg gtagtgaggt tagtcaccca accactcacc
    26821 ggggcgcgaa tttccgtttg cgccagttgc cattgtgtct gtttcagcgt ggcctgtgcg
    26881 gcattaacac tggcctgcat cgctttaaca ttgaggttag cggtatcgag ttcttcggca
    26941 gaaataaaat tttgtgagag atgacgacga cgattcgctt cgttattggc ttttgcgtgg
    27001 tcagattgcg ctttcgccag ctgagcctgt gcgttcagct cggcgatctg aaagggcgtt
    27061 ttatcgatcg tgagcagaag atcccctgct ttaaccagct ggttgtcttt tatattcagc
    27121 tcaacaatac gtcccgacac ctgtggggtg atagaaacct gttctgcgcg tattttcccg
    27181 tcgcgtgtcc acggagattg catgtaataa ttccacagcc accatccggc aagaaccgca
    27241 actaccgcta caatgattgt tgagaaatac ttaattgttt taattgacat agttaccacg
    27301 caatcagtat tgccagagca aggcaaacgc aaatcgcaaa cagcgataaa tccattaaca
    27361 agggatgcca gatgtcaccg gcgtagatcc agccacgaag caggcggtgt acgacaagcc
    27421 agatgacgaa tccaaacgcg aaggctttga aaaacggagg aaagtagacg gacgcaccga
    27481 acaccagatc ttgtaagggc aatcctgttg cattgagcat aaacttcaca aagtagattc
    27541 ctttacgacc gaatatattg cgttagatta ataaatattc tttaagtgcg aaaaatttac
    27601 gcgcaatttc tgaaaaatat gtctgacggt aaccaaatgc agcaatacat ttgttttagc
    27661 aatacaattg ctgcacacta ttctaaaagc cgcataatat cttagcaagc taattataag
    27721 gagatgaaat tggaatcgcc actaggttct gatctggcac ggttggtgcg catatggcgt
    27781 gctctgatag accatcgcct gaaaccgctg gagttaacac aaacccattg ggttacgtta
    27841 cacaatatcc atcagttacc tccagaccag tcgcaaattc aactggcaaa agcgattggc
    27901 atcgagcagc catcactggt ccgtactctg gaccaactgg aagaaaaagg gttaatttcg
    27961 cgtcaaactt gtgccagcga tcgtcgggct aaacgtatta aactgacgga aaaggcagag
    28021 ccactgatca gcgaaatgga agctgttatt aacaaaaccc gcgcggaaat attacatggc
    28081 atctccgcag aggaactgga gcaactgatt acgctcatcg caaaacttga gcataatatc
    28141 attgagttac aggccaaagg gtgaaatgaa gggggcgtgt ggtcagtaac ctgaccacac
    28201 gcaaacttat ttagcgcgga gaaacggtca cctgactgcc attgctggcc agtacgacac
    28261 gttggcccgg agagaaacga gtgttgcctt gtttctgtac caccatgatg gtattaccat
    28321 cgtctttacg aatttccagc tcgacaccct gcgttttgtt cattgcactc tgtacgccct
    28381 gaccagctac gccacctgca acagcgcctg ctgcagtagc cagagaacgc ccggttccgc
    28441 caccaacagt attccccagg aaaccaccaa gaacagcacc gccaattgca ccgataacgt
    28501 tggaatcatc accgccctga atctgtaccg gacgtacgtt aacgatggtg ccatagctga
    28561 cattctgtac ttgtttcgct tcagaagcgg tataaacatc ccctgacagg gtgtcgttat
    28621 taacacaacc gacaagagac agacctacca ttgaaacaac caatacgcgt ttaatcattg
    28681 aaaaatctcc tgttcaccat gaaacgccac gcaagcatcc ctcatggtca aagtatatgg
    28741 catatgtgaa tccatggtta catgttctac caaatatgag caaatcataa tgaaagtagg
    28801 atcaaccaat tataaacaaa acattcatga tgattattga tttgaagata atgttttact
    28861 accaacggta aaaatcggtc attccgcatg gtattcacga aaagtttatg tttgagtggt
    28921 gcgcaaacgt cagattgaaa aaggatgaac tatgaaatcg ggccgcttta ttggcgttat
    28981 gtcaggcacc agccttgatg gtgttgatgt tgtgttggcg acaatagatg aacaccgggt
    29041 cgcacagctg gcaagcttga gctggccgat cccggtgtct ctgaaacagg ctgtactgga
    29101 tatttgccag ggccagcagc ttacactttc gcagtttgga cagcttgata ctcaactcgg
    29161 gcgacttttt gctgatgcgg tcaatgcctt gcttaaggaa caaaacctgc aggcgagaga
    29221 tatagttgcg atcggctgtc acggtcaaac cgtctggcat gaaccgacgg gcgtggcacc
    29281 acacacttta cagattggcg ataacaatca aattgtggca cgcaccggaa ttacggttgt
    29341 cggtgatttt cgccgtcgcg atattgcctt gggaggacaa ggcgcaccgc tggtacctgc
    29401 gttccatcat gccttgctgg ctcactcaac cgagcgacga atggtgctca atattggcgg
    29461 catcgccaat ctgtcactgc tcattcctgg gcagccggtt ggcggctacg ataccggtcc
    29521 tggtaacatg ctgatggatg cctggatctg gcgtcaggcc ggtaaacctt acgataaaga
    29581 tgccgagtgg gcacgggcgg gtaaagttat tctcccactg ctgcaaaata tgctcagcga
    29641 cccatatttc tcgcaacctg caccgaaaag caccggacgc gaatacttta actacggctg
    29701 gctggagcgc catttacgcc attttccggg tgttgatccc cgtgatgttc aggccacact
    29761 ggcagaactc accgccgtga ccatttctga acaagttttg ttgagcggtg gctgcgaacg
    29821 attgatggta tgtggtggag gtagtcgtaa tccgctactc atggcgcgtc tggcggcatt
    29881 actgccaggc acagaagtca ccaccaccga tgccgttggc attagtggcg atgacatgga
    29941 agcattggct ttcgcctggc ttgcctggcg gacgctggcg ggattaccag gaaatctgcc
    30001 ttccgtcact ggcgcaagcc aggagacggt actgggggct attttccctg ctaacccgtg
    30061 atatcagagt taactgaatt tttcttaccg cgtctgccgt tacacttgga acgttaggga
    30121 gggcgtattg ccctccagac caggaaagtc ttcgggaaat gtctatgaaa aaactgttac
    30181 tgatctgttt gccggtgctt ctcaccggct gtagcgcgtt caatcaactg gttgagcaca
    30241 tgcaaaccgc tacgctggaa taccagtgtg atgaaaaacc gttgacggtc aaactgaata
    30301 atccgcgcca ggaagtcagt tttgtttacg ataatcaact actgcatctc aaacagggca
    30361 tttcagcgtc tggtgcacgt tacactgacg gaatctatgt tttctggtcg aaaggcgatg
    30421 aagcgactgt ctataaacgc gaccgcatcg tattgaataa ctgtcagtta caaaatccac
    30481 agcgttgaga tttttccagg ggcggcgcac aatagcgtca cccactgaca atccgtaaag
    30541 aaaaccatgt ctgataacga cgaattgcag caaatcgcgc atctgcgccg tgaatacacc
    30601 aaaggcgggt tacgccgtcg cgatcttccc gccgatccat taaccctttt tgaacgttgg
    30661 ctctctcagg cgtgtgaagc caaactggca gaccctaccg cgatggtggt cgctaccgtg
    30721 gatgaacatg ctcagcctta tcaacgcatc gttttactca aacattacga cgaaaaaggc
    30781 atggtgtttt acaccaacct cggtagccgt aaagcacatc aaattgaaaa taatccgcgc
    30841 gttagcctgc tgttcccgtg gcataccctt gaacgccagg tgatggtgat cggtaaagca
    30901 gaacgacttt cgactctcga agtgatgaaa tattttcata gccgcccgcg tgatagccag
    30961 attggtgcat gggtttcgaa gcagtccagt cgcatttctg cccgcggtat ccttgaaagt
    31021 aaattcctgg agctgaagca gaagtttcaa cagggcgaag tgccattgcc gagcttttgg
    31081 ggcggttttc gcgtcagcct tgaacagatt gagttctggc agggcggtga gcatcgcctg
    31141 catgaccgct ttttgtacca gcgagaaaat gatgcgtgga agattgatcg tcttgcaccc
    31201 tgaaaagatg caaaaatctt gctttaatcg ctggtactcc tgattctggc actttattct
    31261 atgtctcttt cgcatctggc gaaaagtcgt gtaccggcaa aggtgcagtc gttatataca
    31321 tggagatttt gatggcaagc agtaacttga ttaaacaatt gcaagagcgg gggctggtag
    31381 cccaggtgac ggacgaggaa gcgttagcag agcgactggc gcaaggcccg atcgcgctct
    31441 attgcggctt cgatcctacc gctgacagct tgcatttggg gcatcttgtt ccattgttat
    31501 gcctgaaacg cttccagcag gcgggccaca agccggttgc gctggtaggc ggcgcgacgg
    31561 gtctgattgg cgacccgagc ttcaaagctg ccgagcgtaa gctgaacacc gaagaaactg
    31621 ttcaggagtg ggtggacaaa atccgtaagc aggttgcccc gttcctcgat ttcgactgtg
    31681 gagaaaactc tgctatcgcg gcgaacaact atgactggtt cggcaatatg aatgtgctga
    31741 ccttcctgcg cgatattggc aaacacttct ccgttaacca gatgatcaac aaagaagcgg
    31801 ttaagcagcg tctcaaccgt gaagatcagg ggatttcgtt cactgagttt tcctacaacc
    31861 tgttgcaggg ttatgacttc gcctgtctga acaaacagta cggtgtggtg ctgcaaattg
    31921 gtggttctga ccagtggggt aacatcactt ctggtatcga cctgacccgt cgtctgcatc
    31981 agaatcaggt gtttggcctg accgttccgc tgatcactaa agcagatggc accaaatttg
    32041 gtaaaactga aggcggcgca gtctggttgg atccgaagaa aaccagcccg tacaaattct
    32101 accagttctg gatcaacact gcggatgccg acgtttaccg cttcctgaag ttcttcacct
    32161 ttatgagcat tgaagagatc aacgccctgg aagaagaaga taaaaacagc ggtaaagcac
    32221 cgcgcgccca gtatgtactg gcggagcagg tgactcgtct ggttcacggt gaagaaggtt
    32281 tacaggcggc aaaacgtatt accgaatgcc tgttcagcgg ttctttgagt gcgctgagtg
    32341 aagcggactt cgaacagctg gcgcaggacg gcgtaccgat ggttgagatg gaaaagggcg
    32401 cagacctgat gcaggcgctg gtcgattctg aactgcaacc ttcccgtggt caggcgcgta
    32461 aaactatcgc ctccaatgcc atcaccatta acggtgaaaa acagtccgat cctgaatact
    32521 tctttaaaga agaagatcgt ctgtttggtc gttttacctt actgcgtcgc ggcaaaaaga
    32581 attactgtct gatttgctgg aaataatgca ttaagtggaa agggggagtg agaaatcact
    32641 ccccctggtt tttatacagg gaacatgatg aaaaatattc tcgctatcca gtctcacgtt
    32701 gtttatggtc atgcgggtaa cagtgcggca gagtttccga tgcgccgcct gggcgcgaac
    32761 gtctggccgc tgaacaccgt tcaattttct aatcacaccc aatacggcaa atggactggc
    32821 tgcgtgatgc cgcccagcca tttaaccgaa attgtgcaag gcattgccgc cattgataaa
    32881 ttacacacct gtgatgccgt attaagtggc tatctgggat cggcggagca gggtgaacat
    32941 atcctcggta tcgtccgtca ggtgaaagcc gcgaatccgc aggcgaaata tttttgcgat
    33001 ccggtaatgg gtcatccgga aaaaggctgt atcgttgcac cgggtgtcgc agagtttcat
    33061 gtgcggcacg gtttgcctgc cagcgatatc attgcgccaa atctggttga gctggaaata
    33121 ctctgtgagc atgcggtaaa taacgtcgaa gaagcggttc tggcagcgcg cgaactcatt
    33181 gcgcaagggc cacaaattgt gttggttaaa cacctggcgc gagctggcta cagccgtgac
    33241 cgttttgaaa tgctgctggt caccgccgat gaagcctggc atatcagccg tccgctggtg
    33301 gattttggta tgcgccagcc ggtaggtgtt ggtgatgtga cgagcggttt actgctggtg
    33361 aaactgcttc agggggcaac gctgcaggag gcgctggaac atgtgaccgc tgcagtctac
    33421 gaaatcatgg tgaccaccaa agcaatgcag gaatatgagc tgcaagtggt ggctgctcag
    33481 gatcgtattg ccaaaccaga acattacttc agcgcaacaa agctctgaaa tatctcattt
    33541 cggcccacaa cgacctgtgg gccgatggct attactttaa gccttccgct gacagcgcgt
    33601 cttgtacttc cggacgttca gccatacgtt gcataaatgc tgcaatgtgc tcaaaccctt
    33661 ccagattcag tttcaccgcg tatgcccagc gcagaaccgt aaacagatag gcatcagcaa
    33721 ttgtaaatct ttgcccgcag atccagtgct catccttcag tgcctcgttc acatattgca
    33781 gcttcttctc cagctgcgcg cgaactgtcg gtttgtactc ttccggtgta tcaaggcgaa
    33841 acagaggtgt gaaaccttta tgcagctcgg tggcgatgta attcagccat tcgatggttt
    33901 tatagcggga aatactgttt accggtgcca gcaactggcg gtcggggacg ctgtcggcaa
    33961 gatactgcat aatcgctacg ccttccgtca gcaaagtacc gtcatccagc agcaatgcag
    34021 gcacctgccc cttagggtta acggcaaagt aatcgtcacc gttttcgaga cgttttttca
    34081 ttaaatccac actgacgagg gtaaaatcct ttccgctctc acgcagggtg atatgggaag
    34141 cgagagagca ggcacccggt atgtagaaca atttcatcgg taactccttt tgtctgtggt
    34201 ttaaagtatg gtagtgcgtt gctgaacaaa aaaaaagccg ctaacgaaaa gttagcggct
    34261 tgtaaaagag tttccctgaa ttttacgcta cggctgcttt cgccgctttg tctgcagcgt
    34321 catcctgcgt catgcggtgc agtttcggcg cggtcagcag catcagtact gcaataacgg
    34381 cagtagcgac accaatctgc aagaatacgc gaccatagac ttccagtgac atcagcggat
    34441 cggtaacgtt atccggcaca gccatcatac ccgcaacata accaccaatc aggtttgcac
    34501 cggcagtggt caggaaccag ctacccataa tgaagcccat cagacgctgc ggaacgagtt
    34561 gagcaaccat tgccagaccc agaccagaga tcatcagttc cccgatgctc tgcaggccat
    34621 agcttgcgac caaccagctt acagacacga taccagcgtc agacgcgaat ttcgcaccca
    34681 gcggcagaat caggaacgca ccagaacaca tcaccatgcc gattgcaaac ttggttggca
    34741 tcggcagggt atcgcccatc ttgttataga tagcggccag aatcggacta ccgatgatga
    34801 tccagaacgg gttcagtgcc tgatactgct caggttctac ggccagaccc agaatggagt
    34861 gctcaacgtt acgaatcgca aagaagttca gtgacgttgg catctggctg tacagcacga
    34921 agaagataat ggcttcgagc atcaggatga aggcaacgat cattttacga cgcgcagcac
    34981 ctttcatcgc gaaggcttct ttaccgaaga taaccacgat accgaaggca acaacgccca
    35041 gcgccatacg cgcaacttcc tgattgtgca gcagccaggt ggcgatagcg atcagtgcca
    35101 caacaccaat aatggtcagc agcaggttac ggtagttgat aggctcgaag tctggttttg
    35161 aaccgtactg tttaacccag cgttggcaga aggcgaagtt aacgatagtg atcagcaggc
    35221 ctacaacgct caacgcaaac gcaacactcc agccgtattt cgcggccagc cacggcgtag
    35281 caatcataga gaagaaagag ccgatgttga cggacatgta gtacatggtg aatgcaccgt
    35341 ccagacgcgg gtcgtttttc tcatagcatg tagaaagcag agaagacggg ttagctttaa
    35401 acaggccgtt accgaccgca atagccgcca tacccatata aacgataccg gcgtcgtgac
    35461 cagaccaggc aaccagagca taaccaatcg ccagcacaat agcgccgagc ataattacgc
    35521 gtttagtacc cagtaccttg tcacctaacc agccgccgat agcgaccaga ccataaacca
    35581 gggcactaaa ggaagagaaa agggtgattg aatccgcttc agacataccc agttgtttaa
    35641 ccaggtaaac agccataatt ccttgtaggc cgtaataacc aaaacgttcc cataactcaa
    35701 tcgagaagat gagatagaac gccttcggtt gtttgaaagc gttcaaactg acgctttcag
    35761 ttggtttttg gtttgcagtg gacactttta cctctttttt tacatcccat attaacgggg
    35821 gtgctttctc gcgttatgta taaaaaacat acgcgcaatt gttatagtgg gaggggaaac
    35881 ggcaggtaat gttcactatc ttgcactttc agacaagtcc tttgtaataa tctgttacat
    35941 ataactgagg cggggaattc aaccattccg ggaaatgtta tccagcgtta aatttttcaa
    36001 aaaacgtaac ggcagatttt ttcactgcac tgagaggggg gtgatggctt aacggcgata
    36061 tgttctgcta tttagcgttt ttactagtga gatagtccag tttctgaaaa atagccagtg
    36121 taatgttttg taggtcaata tttgttgcgt gtgtaaattt aaatgatttt aacctgattc
    36181 ttgcaatgtt tttacattgc gacagcatcg tgatcttgat cacgaatgaa tagaaaattt
    36241 cgctattaaa aaaacaatta acctgcattt ttggttatcc gcaaacggat tacctgatga
    36301 taacgctaag caatggcatc attgacgtta aaaagatgtt ttaaaagagg ataaagaaag
    36361 gttatcaatg gggtaatcgg tgttacccct tttcttcaga tgtcaacttt ctctttgtat
    36421 tcacaaagat cttcaataat acaagagcca cagcggggct tgcgggcaat gcaggtataa
    36481 cgcccgtgca ggatcaacca atggtggcag tcgactttaa actctgccgg aaccactttc
    36541 agtagctttt cttctacctg ttcgacgttt ttccccggcg caaattgagt acgattacaa
    36601 acgcggaaaa tgtgcgtgtc gacagcaata gtcggccagc cgaatgcagt gtttaatacg
    36661 acgttggctg ttttacgacc tacgccgggc agggcttcaa gcgcagcacg atcttccgga
    36721 acctcgccat tatgctgctc cagcaagata cggcaggttt tgatgatatt ttctgctttg
    36781 ctgttataaa gcccaatcgt tttgatatag gttttcaccc cttcaacgcc cagttcaagc
    36841 atcgctgcag gcgtattcgc caccgggtag agtttcgccg tcgccttatt aacactgaca
    36901 tcggtcgcct gagcggaaag cagtacggca atcagcaatt caaaaggcga actgaaatta
    36961 agctcggtgg tgggatgagg attgttctca cgcaggcgag tgaggatctc caggcgtttt
    37021 gctttgttca tcagacattc cctgtttcac cgtttggcaa tgcacgttcg gcagctgctt
    37081 cagcacggcg ctttttcatt ctttcatcaa tcaggtattt tcctgccagc atcagtccca
    37141 ggccaataaa tgcacctgga ggcagcatcg ccagcaggaa aggggagtcg gtgtggaaaa
    37201 tttccacgcg taatactttt gcccagctac ctaacagcgc atctgcaccg tcaaacaatg
    37261 tgccattgcc gataatttcg cgtagtgaac ccagcacgaa catggcgcag gttgcgccca
    37321 taccaattga aaagccgtcc agtgccgaaa gcgccggacc ttttttggcg gcgaaggctt
    37381 cagcgcggcc cacaacgata cagttagtga caatcagcgg aataaaaatc cctaatgatt
    37441 gatataggcc aaaggcgtag gcgttgatca gcatctgtac agcgctgacc accgaggcga
    37501 tgatcatcac gtaaatggga atgcggatct cggctggcgt ccagtgacgc agcgtcgaaa
    37561 tggtcaggtt ggtcagcgtc agtaccagcg tagtcgcaag tcctaaaccc agagcgttag
    37621 tggcagtgga cgtgaccgcc aacagaggac aaaggccgag caactggacc agagcagagt
    37681 tgtttttcca caacccctga acaataacgt ctttaatttc gctcacgctt tattctccac
    37741 aggcaggaag ttgagaaagt tgtgccggta acgtctgagc gtacaatccg gcgcgtttta
    37801 ccgcattaac caccgcgcgg ggagtaatcg tcgcgccggt gaactggtcg aaatcaccac
    37861 catctttctt caccgcccag tgcgcatcat ccgcaccact gattttttta cccgcaaaat
    37921 gggtgatcca gtcagaaagg cgcagttcga ttttatcgcc aagccctggc gtttcgtggt
    37981 gctctgtcac gcgcgtgcca agtaccgtgc cgttaaaatc ggctcccacc agcagctgaa
    38041 tcgcaccgga atagccatct ggcgcggttg cttccagaac ggcggctacc ggtttgtcat
    38101 cctgtttggc gatgtaaacc cgatgctcac ctttacctaa ctctggcgca gttaccagat
    38161 agcaactctg tgccagcgca ttgttatagc gttcggctgg cagcacctga tcaaataatg
    38221 ccttttgttg cagactggcc tgttcagcaa tcgtcgtttt ggtcatctgg ttgatggccg
    38281 cagttaaccc tgttgaaccc gctgcaaata gcgctaacgt aatgccgtgt tttcggatag
    38341 ttttcagcat ggtttaccct ttgcgatggc cgtagacgcg cggacgcgtg tagtaatcga
    38401 tcagaggaac cgtgatgttc gccagcagga cggcaaaagc cacgccgtca ggatagccgc
    38461 cgaaactgcg gatcaaccag actaataagc ccgccagcgc gccgaaaata agacgaccac
    38521 gattggtcgt agaagcggta accgggtcag tcaaaataaa gaatgcgccg agcatggtcg
    38581 caccagacag cagatgaatt tgcggtgctg ccagtgtttc tggtgagaac aaccagccca
    38641 acgttgcgca taacgccagc gttactaaga agctgagggg aatatgccag cgaatcgctt
    38701 tctgccatag caaccacagg ccgccagcca gccaggcgag atttacccat tgccaaccag
    38761 cgcccgccag aataccgctg tagatcggat attgcataat ctgttcaacc gaatgaccgg
    38821 cacggacaga ggttttaaat gtatccagcg gtgtcgcctg actaatgcca tcaataccta
    38881 agcgtagtgt gttcatatca ccaccactgg cggtatgtcc gctgaaaata acctgtatgg
    38941 cgtcgataaa accagggatg ttgaccgcaa tttcatgtgg cggtaaccag ctggtcatct
    39001 gcaccgggaa ggagatcagt aagaccacat aaccaatcat tgccggatta aacgggtttt
    39061 gccccagacc gccatacaac tgtttagcga tgatcaccgc aaacaccgta cccagcacga
    39121 ccatccacca tggcgcgagg gggggaatac ttaccgccag caataagcct gtcagcaatg
    39181 ctgagttatc tttcaacgtt gcggctaccg actgcttgcg tagtttgagt acgagagctt
    39241 cggctaacag agcactaacc gatgccaaca ggatctgaac gagagtaccc caaccaaaaa
    39301 accacagttg cgctgcgatt cctggcacgg ctgcgagcaa caccaacagc ataatgcgcg
    39361 atgtctggcg ctggttatgg gtataagggg agctagctat tctgaatacc atttagtcct
    39421 cgtttacaac cttctgctgg gcggcttttt tggcctgagc gcgggcaata gccgcggcag
    39481 ctgccgcttt gcgcggatca acctgttctt ctggttctgc gttagtctgt tgctgttcca
    39541 gcttgcgcgc tttggcacgg gcaatagcgg cttcgacggc ggctttgcgc ggatcaacct
    39601 gttcttctgg ttccgcatta gcctgttgct gttccagttt gcgcgctttg gctcgggcaa
    39661 tagcggcttc gacggcggct ttgcgcggat caacctgttc ttctggttcc gcattag
//
LOCUS       DAJOWB010000031        39369 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJOWB010000031 DAJOWB010000000
VERSION     DAJOWB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.031"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(64..2055)
                     /gene="tkt"
                     /locus_tag="OHO85_001800"
     CDS             complement(64..2055)
                     /gene="tkt"
                     /locus_tag="OHO85_001800"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCQ2671180.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2333..3091
                     /gene="loiP"
                     /locus_tag="OHO85_001801"
                     /gene_synonym="yggG"
     CDS             2333..3091
                     /gene="loiP"
                     /locus_tag="OHO85_001801"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCQ2671181.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKALSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(3297..4217)
                     /gene="speB"
                     /locus_tag="OHO85_001802"
     CDS             complement(3297..4217)
                     /gene="speB"
                     /locus_tag="OHO85_001802"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCQ2671182.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4353..5084)
                     /locus_tag="OHO85_001803"
     CDS             complement(4353..5084)
                     /locus_tag="OHO85_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2671183.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGMDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(5230..7206)
                     /gene="speA"
                     /locus_tag="OHO85_001804"
     CDS             complement(5230..7206)
                     /gene="speA"
                     /locus_tag="OHO85_001804"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCQ2671184.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7215..7346)
                     /gene="yqgB"
                     /locus_tag="OHO85_001805"
     CDS             complement(7215..7346)
                     /gene="yqgB"
                     /locus_tag="OHO85_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCQ2671185.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            7482..7697
                     /locus_tag="OHO85_001806"
     CDS             7482..7697
                     /locus_tag="OHO85_001806"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2671186.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            8001..9155
                     /gene="metK"
                     /locus_tag="OHO85_001807"
     CDS             8001..9155
                     /gene="metK"
                     /locus_tag="OHO85_001807"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCQ2671187.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            9590..10984
                     /gene="galP"
                     /locus_tag="OHO85_001808"
     CDS             9590..10984
                     /gene="galP"
                     /locus_tag="OHO85_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCQ2671188.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11061..11558
                     /locus_tag="OHO85_001809"
     CDS             11061..11558
                     /locus_tag="OHO85_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCQ2671189.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            11653..12360
                     /gene="endA"
                     /locus_tag="OHO85_001810"
     CDS             11653..12360
                     /gene="endA"
                     /locus_tag="OHO85_001810"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCQ2671190.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            12440..13171
                     /gene="rsmE"
                     /locus_tag="OHO85_001811"
     CDS             12440..13171
                     /gene="rsmE"
                     /locus_tag="OHO85_001811"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCQ2671191.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13184..14134
                     /gene="gshB"
                     /locus_tag="OHO85_001812"
     CDS             13184..14134
                     /gene="gshB"
                     /locus_tag="OHO85_001812"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCQ2671192.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            14243..14806
                     /locus_tag="OHO85_001813"
     CDS             14243..14806
                     /locus_tag="OHO85_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCQ2671193.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            14806..15222
                     /gene="ruvX"
                     /locus_tag="OHO85_001814"
     CDS             14806..15222
                     /gene="ruvX"
                     /locus_tag="OHO85_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCQ2671194.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(15385..16365)
                     /locus_tag="OHO85_001815"
     CDS             complement(15385..16365)
                     /locus_tag="OHO85_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCQ2671195.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGCVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            16383..17087
                     /gene="yggS"
                     /locus_tag="OHO85_001816"
     CDS             16383..17087
                     /gene="yggS"
                     /locus_tag="OHO85_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCQ2671196.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17105..17671
                     /gene="yggT"
                     /locus_tag="OHO85_001817"
     CDS             17105..17671
                     /gene="yggT"
                     /locus_tag="OHO85_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCQ2671197.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            17668..17958
                     /locus_tag="OHO85_001818"
     CDS             17668..17958
                     /locus_tag="OHO85_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCQ2671198.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            17966..18559
                     /locus_tag="OHO85_001819"
     CDS             17966..18559
                     /locus_tag="OHO85_001819"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCQ2671199.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            18552..19688
                     /gene="hemW"
                     /locus_tag="OHO85_001820"
     CDS             18552..19688
                     /gene="hemW"
                     /locus_tag="OHO85_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCQ2671200.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(19843..20850)
                     /locus_tag="OHO85_001821"
     CDS             complement(19843..20850)
                     /locus_tag="OHO85_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCQ2671201.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(20967..22013)
                     /gene="ansB"
                     /locus_tag="OHO85_001822"
     CDS             complement(20967..22013)
                     /gene="ansB"
                     /locus_tag="OHO85_001822"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCQ2671202.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(22189..22908)
                     /locus_tag="OHO85_001823"
     CDS             complement(22189..22908)
                     /locus_tag="OHO85_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCQ2671203.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(23092..23418)
                     /locus_tag="OHO85_001824"
     CDS             complement(23092..23418)
                     /locus_tag="OHO85_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCQ2671204.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(23418..24137)
                     /gene="trmB"
                     /locus_tag="OHO85_001825"
     CDS             complement(23418..24137)
                     /gene="trmB"
                     /locus_tag="OHO85_001825"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCQ2671205.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            24298..25350
                     /gene="mutY"
                     /locus_tag="OHO85_001826"
     CDS             24298..25350
                     /gene="mutY"
                     /locus_tag="OHO85_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCQ2671206.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            25378..25653
                     /locus_tag="OHO85_001827"
     CDS             25378..25653
                     /locus_tag="OHO85_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCQ2671207.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            25718..26797
                     /gene="mltC"
                     /locus_tag="OHO85_001828"
     CDS             25718..26797
                     /gene="mltC"
                     /locus_tag="OHO85_001828"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCQ2671208.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            26999..28255
                     /gene="nupG"
                     /locus_tag="OHO85_001829"
     CDS             26999..28255
                     /gene="nupG"
                     /locus_tag="OHO85_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCQ2671209.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(28305..30440)
                     /gene="speC"
                     /locus_tag="OHO85_001830"
     CDS             complement(28305..30440)
                     /gene="speC"
                     /locus_tag="OHO85_001830"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCQ2671210.1"
                     /translation="MKSMNIAASSDLVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVEPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTMRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            30838..31536
                     /locus_tag="OHO85_001831"
     CDS             30838..31536
                     /locus_tag="OHO85_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCQ2671211.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWFA"
     gene            31642..31717
                     /locus_tag="OHO85_001832"
     tRNA            31642..31717
                     /locus_tag="OHO85_001832"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31675..31677,aa:Phe,seq:gaa)
     gene            31894..33078
                     /locus_tag="OHO85_001833"
     CDS             31894..33078
                     /locus_tag="OHO85_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2671212.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQATDILLQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSIQ
                     ES"
     gene            33228..34238
                     /locus_tag="OHO85_001834"
     CDS             33228..34238
                     /locus_tag="OHO85_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCQ2671213.1"
                     /translation="MSLKAIATALGVSVTTVSRALGGYADVSASTRARVEAEARRRGY
                     RPNTQARRLKTGKTDAVGLVYPGRDVPFNSGVFMDMVSCISRELAHHDIDLLLIADDE
                     HADCHSYMRLVESRRVDALIIAHTLDDDPRITHLHKAGIPFLALGRVPSGLPCAWFDF
                     DNHAGTGQATQKLIALGHKRIALLSEDTSHSYVVARRQGWLDALNEHELEDTWLRLVS
                     PTRRAGYQAVMELMSLPIPPTAIITDNDLSGDGAAMALQLSGRLSGKDAVSLVVYDGL
                     PQDSIIELDVAAVIQSTRSLVGRQISEMIYQIITDSSSKPLQVVWNPVFYPGKTIHPP
                     CS"
     gene            34334..36460
                     /locus_tag="OHO85_001835"
     CDS             34334..36460
                     /locus_tag="OHO85_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCQ2671214.1"
                     /translation="MVSKYCRLSSPRSDLIIKTRPHAEIIWWGSALKHFSPDDCASLE
                     RPVANGRLDIDTPLTLMAENALGLFSSPGLEGHRNGLDASPVFYTVDVEHTENTLRLT
                     SEDSVAGLRLVSELVMTPSGILKVRHALTNLREGDWQINRFAITLPLAERAEEVMAFH
                     GRWTREFQPHRVRLTHDAFVLENRRGRTSHEHFPALIVGTPGFSEQQGEVWAVHLGWS
                     GNHRMRCEAKTDGRRYVQAEALWMPGEKALRKNETLYTPWLYACHSADGLNGMSQQYH
                     RFLRDEIIRFPEQKPRPVHLNTWEGIYFNHNPDYIMQMAERAAALGVERFIIDDGWFK
                     GRNDDRAALGDWYTDEQKYPNGLMPVIKHVKSLGMEFGIWVEPEMINPDSDLFRLHPD
                     WVLSMPGYSQPTGRYQYVLNLNIPEAFAYIYERFLWLLGEHPVDYVKWDMNRELVQAG
                     HEGRAAADAQTRQFYRLLDLLRERFPHVEFESCASGGGRIDFEVLKRTHRFWASDNND
                     ALERCTIQRGMSYFFPPEVMGAHIGHRRCHATFRQHSIAFRGLTALFGHMGLELDPVA
                     ADAKESDGYRRYALLYKEWRQLIHTGVLWRVDMPDPSIQVQGVVSPDQSQALFMISQL
                     AMPDYTLPGILRFPGLAAEVRYRLRVIDHPDLQVVGEGGHTMRKLPVWMNQSLEASGE
                     WLAQGGIQLPVLDPESAILIALERAV"
     gene            36523..37800
                     /locus_tag="OHO85_001836"
     CDS             36523..37800
                     /locus_tag="OHO85_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2671215.1"
                     /translation="MNSASTHKNPDFWIFGLFFFLYFFIMATCFPFLPVWLSDVVGLS
                     KTDTGIVFSCLSLFAISFQPLLGVISDRLGLKKNLIWSISLLLVFFAPFFLYVFAPLL
                     RFNIWAGALTGGVFIGFVFSAGAGAIEAYIERVSRSRGFEYGKARMFGCLGWALCAAM
                     AGMLFNVDPSLVFWMGSGSALLLLLLLFLARPSTSQTAMVMNTLGANSSLISTRMVFS
                     LFRMRQMWMFVLYTIGVACVYDVFDQQFATFFRSFFDTPQAGIKAFGFATTAGEICNA
                     IIMFCTPWIIHRIGAKNTLLVAGGIMTIRITGSAFATTATEVVILKMLHALEVPFLLV
                     GAFKYITAVFDTRLSATVYLIGFQFSKQLAAILLSTFAGHLYDRMGFQNTYFVLGMIA
                     LTVTVISVFTLSSSRGSVHPSVEKAPAAHSEIN"
     gene            37797..39230
                     /locus_tag="OHO85_001837"
     CDS             37797..39230
                     /locus_tag="OHO85_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCQ2671216.1"
                     /translation="MMKQRLALAQSALEKLCARRGNAWYPIFHLAPPAGWMNDPNGLI
                     YFNGRYHAFFQHHPASAYQGPMHWGHATSTDMLHWQHEPIALAPGDKYDRDGCFSGSA
                     VDDDGVLSLIYTGHICLDDRGNDSIIREVQCLATSHDGIHFEKQGCVLTPPEGIMHFR
                     DPKVWHEDGSWWMVIGARDASDNGQVLLYRGTSLRDWHLEHVLAHSAAGKSYMWECPD
                     FFRCGNFHWLMFSPQGMPPSGYRFRNLFQSGVLAGSWKPGSVFALKGGFEELDYGHDF
                     YAPQSMLAEDGRRIIMAWMNMWDSPVPTRSEAWAGCLTLPREVFERDGRLCQRPVREV
                     ESLRKKCQPLSPVRLQGLQLLTENVQAAELLVTWHTVDSHAEHYGVRLGDGLRLYVDN
                     QAGRLVLWRYYPEEGLDGYRSVELPDTEYLTLRIFLDRSSVEVFVNDGEATLSSRIYP
                     QADSRQLSLYAAHGDAILTDGTLWMLT"
BASE COUNT         9391 a        10150 c        10020 g         9808 t
ORIGIN      
        1 caaacggaca tatcaaggta ataaaaaagg tcgccgaagc gaccttttta cccgaaatgc
       61 taattacagc agttcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc
      121 aaacagcagc tctgccggag cagattcacc gaaggtggtc ataccgacga tagcaccgtt
      181 caggccaaca tacttgtacc agtagtcagc aatacccgct tctacagcaa cgcgtgcagt
      241 aaccgctttc ggcagtacgg attcacggta agcagcatcc tgcttgtcaa atgcgtcggt
      301 agacggcatg gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc
      361 aacagccagt tcaacttctg aaccggtagc gatgaaaatc agttccggct gaccggcgca
      421 gtctttcagc acataaccac cgcgcgcgat gtttgccagt tgctcttcag ttcgttcctg
      481 ctgcgccagg ttctgacggg agaggatcag tgcggtcggg ccgtcctgac gctcaacacc
      541 gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg
      601 ggttacgcgc agagaagcga cctgctcaac cggctggtga gtcgggccgt cttcgcccag
      661 accgatggag tcgtgggtgt aaaccatcac ctgacgctgt ttcatcagcg cagccatacg
      721 tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaaacc
      781 accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg
      841 gatgtagtta cccgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt
      901 agacggtgcc aggtcagcag aaccgccgag gaattccggc aacagcggac cgaaagcttc
      961 gatagcattc tgagacgctt tacggctggc gattttcgcc gggttagcct gcagtttagc
     1021 aatgaactct tttgctttcg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt
     1081 aaattcagcg gcttcctgcg gataagcttt cgcgtaagca gcgaatttct cgttccatgc
     1141 ggattctttc gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat
     1201 ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc
     1261 cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat
     1321 ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt ctactgcgcg
     1381 tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc
     1441 gaaacgcatt gcggtgtcgt cggtgaacca gccttcaacg tgaccatcaa tagagatacc
     1501 gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac
     1561 ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc
     1621 gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat
     1681 acctactgcg ttggcaatac cctgccccag cggaccggtg gtggtttcca caccagcggt
     1741 gtaacccact tccgggtggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc
     1801 ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg
     1861 gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt
     1921 caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg
     1981 accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc
     2041 tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg
     2101 acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg
     2161 tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg
     2221 cgcaatctac tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac
     2281 ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat
     2341 tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga
     2401 ctccaacgga ctgctttcat caggagcgga agcttttcag gcttacagtt tgagtgatgc
     2461 gcaggtgaaa gccctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc
     2521 gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa
     2581 tatcaacggt cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc
     2641 aatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga cggataacga
     2701 agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg
     2761 gatgcaggtg gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat
     2821 tgtcggcagt ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca
     2881 attctcccag cgccaggaag cagaagccga tgattattct tacgatcttc tacgccaacg
     2941 cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaaac tggaagaagg
     3001 ccgccaaagc tcaatgtttg acgaccatcc ggcatccgcc gaacgcgccc agcatattcg
     3061 cgatcgtatg agcgcggatg gggttaagta aagcctggtg gtgtcgaacg taggtcagat
     3121 aaggcgttca cgccgcatct gacatgaatg gcacatttgt taccttgtgc gcattgccgg
     3181 atgcgatgct ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt
     3241 tcacgccgca tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac
     3301 tcgccctttt tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga
     3361 gcggtgattt ccgactgatc atatgccgga gccacttcca ctacgtccat cccaacgatg
     3421 ttgagatctt tcaggccgcg taccagttta atagcgcgat cggaggtcag gccgccaatc
     3481 actggcgtac cggtgcctgg tgcaaaagca ggatccaggc agtcgatatc aaaggtcagg
     3541 taaaccggca tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctacga
     3601 tcgttcacct ggcaggcgtc cagtacggta aagccgttgt ctttatcaaa ctcggtacga
     3661 ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac
     3721 atggtgccgt ggtcaaattc acaaccgttc gcgtaggtat cggtgtgggc gtcgaagtgt
     3781 accagcgcca ttttgccgaa atgcttagca tgagcacgca gcagcggcag cgtaacaaag
     3841 tggtcaccac cgaaagagag catacgctta ccggcagcca gcaacttctc ggcgtgtgcc
     3901 tgcagttttt cgctcatctc acgggcatcg ccgaaggcat ataccagatc accgcagtcc
     3961 acgacgttca ggcgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc
     4021 agattcgtcg aaacctgacg gatcgctgcc ggaccgtgac gaccaccggc acggccagag
     4081 gtggccatgt cgaacggcac gccggtgatc acccagtcgg catcgctgtc atacggctgg
     4141 aagttcatcg gcaggcgtaa aaaaccaaag gcgttggaaa ccagtgagtt atcgtattga
     4201 tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg
     4261 cgtcgttagg cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc
     4321 gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag
     4381 ccattgttat ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg
     4441 ccttgtaatt caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc
     4501 gaattggctg cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc ggtatgttta
     4561 tctttgcgga atcctttaat cgctaacgaa tctccggcat tcacaatata gccgttgtta
     4621 ttcagcgaac cttgcttgcc gttcagcaca tccatgccat caaccgtagc aacgatctcg
     4681 tagttggtgt tacgactgta attacggaca tataattgat aattcattcc gactctggca
     4741 tggacaatat agcgtggttc attattggat gaatcataaa cccgcgtaat aggtagagaa
     4801 ttaaaattac catcgcgaat agcatattcc agttcgttaa tacgaatgct ataaacgcgg
     4861 tcgtagccgg aaggaacatt agtcgaataa ctaataacga tcatctctgc cggatcgcgc
     4921 caggctcgct cagcagatac gccctgaacc gttgagtgca catccccacc ccaggcagtg
     4981 ccgacattgc ccgatgcact gctattatac tgcgcattac tggaacaacc ggccagcagc
     5041 acgattaatg ccaccaacgc gctacgtact ttccattttt tcataaccgt tccctctgca
     5101 tttaattaat gcagcgattg tagggagatt atttaataag aaaagcagcg agattatttt
     5161 taagaatata taaaaagaaa gcgggccgat ttacgtaaat aaatcgaccc gccagcggaa
     5221 taatcttaat tattcatctt caagataggt ataaccgtac aaacctgcct cgaactcttc
     5281 aaggaactgt tgttgcagtt cagcatcaag atcggttttc ttcacttgat cgcggaactg
     5341 ggttaacagc gttttcggat cgagttgtac atattgcagc atgtccgcca cggtatcgcc
     5401 ttcgtcagac agttctactt ctacgctacc gtcagggaac acgaacacgt caaccgcttc
     5461 ggtatcaccg aacaggttgt gcatattgcc gaggatctcc tgatatgcgc cgaccataaa
     5521 gaaaccgagc atcggcggat tctctggatc gtactccggc attggcattg tcgtggcaat
     5581 accatcacca tcaatatagt ggtcgatagc accgtcagag tcacaggtaa tatccagcag
     5641 cacagcgcga cgttccggca cttgatccaa cccttccagc ggcagaaccg ggaacaactg
     5701 gtcgatcccc cacgcatccg gcatcgactg gaacagcgag aagttgacgt acattttgtc
     5761 cgccatacgt tcctgcagct cgtcgataat cggacgatga gcacggtttt gcggatccag
     5821 ttgcttctgc acttcatggc acatgctcag atagagttgt tctgcccatg cacgttcttg
     5881 caggctaaag gtgccggaag agtagccgat atgaatgtcg tgcagatcca tctgactgtc
     5941 gtgtaaccat tcacgcagag aacggcgagt tcccggttcg tgcatctcct gccaggtttc
     6001 ccacatgctt tgcagcgcgc gcggcgcatc ttctgcaggc gcggtcggca ccgtgtattc
     6061 gttacgttcc acgccgatga tattagatac cagcacggtg tgatgcgcag tcaccgcacg
     6121 acccgattcg gtgattaccg tcggatgcgg cagaccgttt tcttcacacg catcaccaat
     6181 cgcccagatg atgttgttgg catattcatt gaggccgtag ttcaccgaac agtcggactg
     6241 cgaacgggtg ccttcatagt ccacgcccag accgccgccg acgtcgaagc actgaatatt
     6301 gacgcccagc ttgtgcagtt ccacatagaa acgcgcggat tcacgaacgc ctgtcgcgat
     6361 atcgcgaata ttcgccatct gcgaaccgag gtggaagtgc agcagttgca ggctgtcgag
     6421 acgcccggct tcacgcaggg tttcaacaag ttgcagtacc tgagtcgcag ccaggccgaa
     6481 cttcgatttt tccccgccgg aggactgcca tttacccgag ccctgcgaag ccagacgtgc
     6541 acgcacgccc agacgaggaa cgacattcag acgttctgct tcatccagca caatggcgat
     6601 ttctgacatc ttctcaatga ccagatagac cttgtgcccc atcttctcgc caattaatgc
     6661 caggcggata tattcgcggt ctttataacc gttgcagacg atgacgctac gggtcatgcc
     6721 agcatgcgcc agcaccgcca tcaactctgc tttcgagcct gcttccagcc ccaacggttc
     6781 gccagaatga atcagggact caatcacgcg gcggtgctgg ttaactttga tcggataaac
     6841 aaggaagtaa tcgccgttat agccgtagga ttcccgcgca cgtttgaacg cggcgttaat
     6901 ggaacgcaaa cggtgctgca ggatctgtgg gaaacagaac agtgcaggca gacgctgacc
     6961 ctgtgcttca cgagttttca ctaactgcgc gagatcgacg cgagcttccg ggacgtccgg
     7021 gtccgggcac acgctgatgt ggcccagctc gttaacgtca tagtagttat tgccccacca
     7081 ggcaatattg taagtacgca gcatcttgct ggcttcctgg gagctcattg caacctcctg
     7141 catggagcgt agtacaccgt gttcgcccgc tgacgaaggc aaacccatag acatgtcgtc
     7201 agacatagcg aacctcaaat tattttatta agtgtaaaac agttaacgac tatcgcagcc
     7261 tggaactgcg ataacaaccc ataagcacat ggattttcca gcagtgaatg ctgacgctcc
     7321 aactgcgcaa ccggtttctt tttcataaca ttattaagca cataaccgaa cgtaagtgtg
     7381 acagttcggc gaaaccacga gaaaactctt gtttttacaa gagcgccctt gttcagtcct
     7441 cagtaactgt aaccagctct tgaatcctga gaagcgccga gatgggtata acatcggcag
     7501 gtatgcaaag cagagatgca gagtgcgggg aacgaatctt caccagaacg gtgagacagg
     7561 ttaagcagca gacaacggtt cattatttcg tatcacctcc acggccgcct gttaagacga
     7621 acccacaagc caaaactctg atttcaaccc ggctggaagt ggcaacacga aagaaacgtc
     7681 gtgtgctttt tatttaagcc gcgcgccgcg ttttataccc cacaatggca gaaaattgca
     7741 aaagataaat acgcagaatg ccggcattgt caggaaaaat ttccagccac gtttttaaca
     7801 gaatgagaca cgattcaaaa aaaagtggaa atcgggtgaa gaattgacct aaaatagcca
     7861 tccagatgtt aatccatcca taccgattaa cactcagact gccagtgttt ttaacttgca
     7921 gagtcgtggt aggatccgct accacagaaa atccacacaa cagtttgagc taaccaaatt
     7981 ctctttaggt gatattaaat atggcaaaac acctttttac gtccgagtcc gtctctgaag
     8041 ggcatcctga caaaattgct gaccaaatct ctgatgccgt tttagacgcg atcctcgaac
     8101 aggatccgaa agcacgcgtt gcttgcgaaa cctacgtaaa aaccggcatg gttttagttg
     8161 gcggcgaaat caccaccagc gcctgggtag acatcgaaga gatcacccgt aacaccgttc
     8221 gcgaaattgg ctatgtgcat tccgacatgg gctttgacgc taactcctgt gcggttctga
     8281 gcgctatcgg caaacagtct cctgacatca accagggcgt tgaccgtgcc gatccgctgg
     8341 aacagggcgc gggtgaccag ggtctgatgt ttggctacgc aactaatgaa accgacgtgc
     8401 tgatgccagc acctatcacc tatgcacacc gtctggtaca gcgtcaggct gaagtgcgta
     8461 aaaacggcac tctgccgtgg ctgcgcccgg acgcgaaaag ccaggtgact ttccagtatg
     8521 acgacggcaa aatcgttggt atcgatgctg tcgtgctttc cactcagcac tctgaagaga
     8581 tcgaccagaa atcgctgcaa gaagcggtaa tggaagagat catcaagccg attctgcccg
     8641 ctgaatggct gacttctgcc accaaattct tcatcaaccc gaccggtcgt tttgttatcg
     8701 gtggcccgat gggtgactgc ggtctgactg gtcgtaaaat tatcgttgat acctacggcg
     8761 gcatggcacg tcacggtggc ggagcattct ctggtaaaga tccatcaaaa gtggaccgtt
     8821 ccgctgcgta cgcagcacgt tatgttgcaa aaaacatcgt tgctgctggc ctggccgatc
     8881 gttgtgaaat tcaggtttcc tacgcaatcg gcgtggctga accgacctcc atcatggtag
     8941 aaactttcgg taccgagaaa gtgccttctg aacaactgac tctgctggta cgtgagttct
     9001 tcgacctgcg cccatacggt ctgattcaga tgctggatct gctgcacccg atctacaaag
     9061 aaaccgcagc atacggtcac tttggtcgtg aacatttccc gtgggaaaaa accgacaaag
     9121 cgcagctgct gcgcgatgct gccggtctga agtaatcttt cttcacctgc tttcaaaggc
     9181 cagcctcgcg ctggcctttt tcttttacct caccgcggtg gataggcatt cacgccgcat
     9241 ccggcaaaaa gctgcccgca caataacatc attcttcctc aacacgtttt atcgcaaatt
     9301 atcatcacta ctgaaaccga ttacaccaat aacaccagcc aatatttgta atattttcat
     9361 attattattc agttttcaca gttgttacat ttcttttcag taaagtctta attgcagata
     9421 acagcgttta atctatgatg atataactca attattttca tgcacttaaa ccataactaa
     9481 gataaatgtt agtgtaagcg attacactga tgtgatttgc ttcacatctt tttacgtcgt
     9541 actcacctat cttaattcac aataaaaaat aaccatattg gagggcatca tgcctgacgc
     9601 taaaaaacag gggcggtcaa acaaggcaat gacttttttc gtctgcttcc ttgccgctct
     9661 ggcgggatta ctctttggcc tggatatcgg tgtaattgct ggcgcactgc cgtttattgc
     9721 agatgaattc cagattactt cgcacacgca agaatgggtc gtaagctcca tgatgttcgg
     9781 tgcggcagtc ggtgcggtgg gcagcggctg gctctccttt aaactcgggc gcaaaaagag
     9841 cctgatgatc ggcgcaattt tgtttgttgc cggttcgctg ttctctgcgg ctgcgccaaa
     9901 cgttgaagta ctgattcttt cccgcgttct gctggggctg gcggtgggtg tggcctctta
     9961 taccgcacca ctgtacctgt cagaaattgc gccggaaaaa attcgcggca gtatgatctc
    10021 gatgtatcag ttgatgatca ctatcgggat cctcggtgct tatctttctg ataccgcctt
    10081 cagctacacc ggtgcatggc gctggatgct gggtgtgatt atcatcccgg caattttgct
    10141 gctgattggt gtcttcttcc tgccagacag cccacgttgg tttgccgcca agcgccgttt
    10201 tgttgatgcc gaacgcgtgc tgctacgcct gcgtgacacc agcgcggaag cgaaacgcga
    10261 actggatgaa atccgtgaaa gtttgcaggt taaacagagt ggctgggcgc tgtttaaaga
    10321 gaacagcaac ttccgccgcg cggtgttcct tggcgtactg ttgcaggtaa tgcagcaatt
    10381 caccgggatg aacgtcatca tgtattacgc gccgaaaatc ttcgaactgg cgggttatac
    10441 caacactacc gagcaaatgt gggggaccgt gattgtcggc ctgaccaacg tacttgccac
    10501 ctttatcgca atcggccttg ttgaccgctg gggacgtaaa ccaacgctaa cgctgggctt
    10561 cctggtgatg gctgctggca tgggcgtact cggtacaatg atgcatatcg gtattcactc
    10621 tccgtcagcg cagtatttcg ccatcgccat gctgctgatg tttattgtcg gttttgccat
    10681 gagtgccggt ccgctgattt gggtactgtg ctccgaaatt cagccgctga aaggccgcga
    10741 ttttggcatc acctgctcca ccgccaccaa ctggattgcc aacatgatcg ttggcgcaac
    10801 gttcctgacc atgctcaaca cgctgggtaa cgccaacacc ttctgggtgt atgcggctct
    10861 gaacgtactg tttatcctgc tgacattgtg gctggtaccg gaaaccaaac acgtttcgct
    10921 ggaacatatt gaacgtaatc tgatgaaagg tcgtaaactg cgcgaaatcg gcgctcacga
    10981 ttaatctcct caggcttcct cccatcgcgg aggaagccac ctcttgcagt catcttttct
    11041 tcgctctatc ctctgccgct atgaaaacat cccgtctccc tatcgccatc cagcaggccg
    11101 ttatgcgccg cctgcgggaa aaactcgccc aggccaacct gaagctaggg cgtaactacc
    11161 cggagccaaa actctcttac acccagcgcg gaacctccgc cggaacagcc tggctggaaa
    11221 gctatgaaat ccgcctcaat cccgttttgc tgttggaaaa cagtgaagct tttattgaag
    11281 aagtggtacc gcacgaactg gcacatttgc tggtatggaa acatttcggc cgcgtagcgc
    11341 cacatggcaa agagtggaag tggatgatgg aaagcgtgct gggcgttccc gcccgtcgta
    11401 cgcatcagtt cgaactgcaa tccgtgcgtc gcaacacctt cccctaccgc tgcaagtgcc
    11461 aggagcatca gcttaccgta cgccgccata atcgcgtagt tcgtggcgag gccgtctatc
    11521 gctgtgttca ctgcggtgaa cagctggttg cgaaataacc atctgaacta tcaggaactt
    11581 tcctgatctg gctgattgca taccaaaaca actttcgcta cgttgctggc tcgttttaac
    11641 acggagtaag tgatgtaccg ttatttgtct attgctgcgg tggtactgag cgcagcattt
    11701 tccggcccgg cgttggccga aggtatcaat agtttttctc aggcgaaagc cgcggcggta
    11761 aaagtccacg ctgacgcgcc cggtacgttt tattgcggat gtaaaattaa ctggcagggc
    11821 aaaaaaggcg ttgttgatct gcaatcgtgc ggctatcagg tgcgcaaaaa tgaaaaccga
    11881 gccagccgcg tagagtggga acacgtcgtt cccgcctggc agttcggtca ccagcgccag
    11941 tgctggcagg acggtggacg taaaaactgc gctaaagatc cggtctatcg caagatggaa
    12001 agcgatatgc ataacctgca gccgtcagtc ggtgaggtga atggcgatcg cggcaacttt
    12061 atgtacagcc agtggaatgg cggcgaaggc cagtacggtc aatgcgccat gaaggtcgat
    12121 ttcaaagaaa aagctgccga accaccagcg cgtgcacgcg gtgccattgc gcgcacctac
    12181 ttctatatgc gcgaccaata caacctgaca ctctctcgcc agcaaacgca gctgttcaac
    12241 gcatggaaca agatgtatcc ggttaccgac tgggagtgcg agcgcgatga acgcatcgcg
    12301 aaggtgcaag gcaatcataa cccgtatgtg caacgcgctt gccaggcgcg aaagagctaa
    12361 cctacactag cgggactctt tttgttaacc cctaccccac gcgtacaacc gcgtggggag
    12421 acgacgcgga tttttaacta tgcgtatccc ccgcatttat catcctgaac cactgaccag
    12481 ccattctcac atcgcgcttt gcgaagatgc cgccaaccat atcgggcgcg tactgcgcat
    12541 ggggccgggg caggcgttgc aattgtttga cggtagcaac caggtctttg acgccgaaat
    12601 taccagcgcc agcaaaaaaa gcgtggaagt gaaggtgctg gaaggcaaga tcgacgatcg
    12661 cgaatctccg ctgcatattc acctcggtca ggtgatgtcg cgtggtgaaa aaatggaatt
    12721 tacgatccag aaatcgatcg aactcggtgt aagcctcatt acgccacttt tttctgagcg
    12781 ctgcggcgtt aaactggata gtgaacgtct gaacaagaag ctccagcagt ggcagaagat
    12841 tgcaattgct gcctgtgagc agtgtggtcg taaccgggtg ccggaaatcc gcccagcgat
    12901 ggatctggaa gcctggtgtg cagagcagga tgaaggactg aaactgaatc ttcacccgcg
    12961 cgccagtaac agcatcaata cgttgccgtt accggttgaa cgcgtccgcc tgctgattgg
    13021 cccggaaggc ggtttatcgg cagatgaaat tgccatgact gcccgctatc aatttactga
    13081 tatcctgttg ggacctcgcg ttttgcgtac agagacaact gcgctcaccg ccattaccgc
    13141 gctacaagta cgatttggcg atttgggcta acggagaaga ataatgatca agctcggcat
    13201 cgtgatggac cccatcgcaa acatcaacat caagaaagat tccagtttcg ctatgttgct
    13261 ggaagcacag cgtcgtggtt acgaacttca ctatatggag atgggcgatc tgtatctgat
    13321 caatggcgaa gcccgcgccc atacccgcac gctgaacgtg aagcagaact acgaagagtg
    13381 gttttcattc gtcggtgaac aggatctgcc gctggccgat ctcgatgtga tcctgatgcg
    13441 taaagacccg ccgtttgata ccgagtttat ctacgcgacc tatattctgg aacgtgccga
    13501 agagaaaggg acgctgatcg ttaacaagcc gcagagcctg cgcgactgta acgagaaact
    13561 gtttaccgcc tggttctctg acttaacgcc agaaacgctg gttacgcgca ataaagcgca
    13621 gctaaaagcg ttctgggaga aacacagcga catcattctt aagccgctgg acggtatggg
    13681 cggcgcgtcg attttccgcg tgaaagaagg cgatccaaac ctcggcgtga ttgccgaaac
    13741 cctgactgag catggcactc gctactgcat ggcgcaaaat tacctgccag ccattaaaga
    13801 tggcgacaaa cgcgtgctgg tggtggatgg cgagccggta ccgtactgcc tggcgcgtat
    13861 tccgcagggg ggcgaaaccc gtggcaatct ggctgccggt ggacgcggtg aacctcgtcc
    13921 gctgacggaa agtgactgga aaatcgcccg tcagatcggg ccgacgctga aagaaaaagg
    13981 gctgattttt gttggtctgg atatcatcgg cgaccgtctg actgaaatta acgtcaccag
    14041 cccaacctgt attcgtgaga ttgaagcaga gtttccggtg tcgatcaccg gaatgttaat
    14101 ggatgccatc gaagcacgtt tacagcagca gtaacccacc ttagcgagaa ggatctcgtt
    14161 gagactctga gtgacagcgc ccttctttcc acgcatactg ggcgctgttg cttttttgaa
    14221 ccaggaaaca gaacctctga caatgaattt acagcatcac tttcttattg ccatgcctgc
    14281 tctccaggat ccgattttcc gtcgttccgt ggtctacatt tgcgaacata ataccaatgg
    14341 tgcaatgggg atcatcgtca acaagccgct ggaaaatctc aaaattgaag ggattctgga
    14401 aaagctgaag atcacgccgg agccgcgtga tgaatcaatc cgtctggata aaccggttat
    14461 gctcggcggt ccgctggctg aagatcgcgg gtttattttg catactccgc cctccaattt
    14521 tgcttccagt attcgcattt cagacaacac agtgatgacc acttcccgcg atgtgctgga
    14581 aacgctcggc accgataaac aaccgtctga cgtattggtg gctctgggtt atgcctcctg
    14641 ggaaaaaggt caactggaac aagaaattct cgataacgcg tggctaacgg ccccggcgga
    14701 tctgaatatt ctgttcaaaa caccgattgc cgaccgctgg cgcgaggcgg caaaactgat
    14761 tggtgtggat attctcacca tgcctggtgt ggcagggcac gcctgatgag tggaacctta
    14821 ctcgccttcg acttcggtac caaaagcatt ggtgtagcgg tcggccaacg cattaccggc
    14881 accgctcgcc ctttgcctgc aattaaagca caggacggta cgccggactg gaacattatc
    14941 gagcgtttac tgaaagagtg gcagccggac gaaatcatcg tcggtttgcc gctgaatatg
    15001 gacggcaccg agcaaccatt gactgccaga gcgcgtaaat ttgccaaccg tattcatggt
    15061 cgtttcggtg ttgaagtaaa gctccatgac gaacgtctta gcaccgtgga agcccgttcc
    15121 ggtctgtttg aacagggcgg ctatcgggcg ctaaataaag gcaaagtcga ctctgcctct
    15181 gcggttatta ttctcgaaag ctatttcgag cagggatatt aaagcgattt aaacgcctgc
    15241 tggagtgtat aatatcatcg cgccttacgc cttatccggc ctacatgtgg cagcggttgt
    15301 aggtctgata agacgcgcaa gcgtcgcatc agacgtttat tgcctttgta ggcccgataa
    15361 gcttgcacat cgggcatggc aacgtcacaa acgcccttcc cccactcgct gctgaaaact
    15421 ctgctgaaac gttatcatcc ccacctgctg cccggtttga ataacatgcg gtaactggtg
    15481 ggttttccct tcgcgaatca aattccccac cgcgggagtg ttaatcaata attcaaacag
    15541 cgccacgcat ccttcctgtt tatccacttc cagcttttgt gacaacacgg cccgtaaact
    15601 acctgccagt tgattacgta cgggatcttt ttcctgcgcc ggaaatgaat ccaccagtcg
    15661 ctcaactgcc tgcgcggcac cacgcgtatg taaggttgcc agcaccagat gcccggtttc
    15721 tgccgccgtt agcgccagac ggattgtctc actgtcacgt agctctccga gcaaaatcac
    15781 atcaggatct tcccgcaatg cagcccgcaa ccccgatgcg aaagtcatac agtgcaaacc
    15841 aatttcccgc tgctggatca aacatcgctg gctggcatag agatattcca caggatcttc
    15901 cagcgtcaga atatgcgcat cggcatgttg attaagatag ccaaccatcg ccgccagcgt
    15961 ggtagatttg ccgctccccg tcgcccccgt caccagaatc aggccattct cgctcttgag
    16021 taattccggc aataccggtg gcgcaccaag ctgttcgagc tgtggacagt gcgaaggtaa
    16081 caaccgtaat gccagagaaa taccttgccg ttgcgcgaac gcactgccac gcaaccgctg
    16141 gttttccgcc agcgacacgg caaaatccag ctggccattt tccagcaata ttgcccgctg
    16201 atcgtcatcc agccactccc gcagtagctc ttcgacgtcc ggcgcatcaa acggcgcagc
    16261 ttccattctg ccacgaatgc gccatcgtgc gggccaggcg ctgcacaggt gtagatccga
    16321 gacgttatgc tttacactaa gggccacaat ttcttccata ttcatactaa gatcctcgga
    16381 aaatgaacga tattgcgcat aacctggcac aggtccggga caaaatctca gcggctgcaa
    16441 cgcgttgcgg ccgttctcca gaagaaatta cgctgcttgc agtcagtaaa acaaaacctg
    16501 cgagcgccat cgcagaagcc attgatgccg ggcagcgtca atttggtgaa aactacgttc
    16561 aggaaggggt agataaaatt cgccactttc aggaactggg cgtaacagga ttagaatggc
    16621 attttattgg cccgttgcag tctaataaaa gccgcctggt ggcagagcat ttcgactggt
    16681 gtcataccat cgaccgtttg cgcattgcta cccgcctcaa cgatcagcgc ccggcagaac
    16741 ttccccctct taacgtactg attcaaatta atattagtga tgaaaacagt aagtccggga
    16801 ttcaactggc agaactggac gagctggcag cggcggtcgc tgaactaccg cgtttacgtc
    16861 tgcgcgggct gatggcaatc cctgcgcctg agtcagaata tgtaaggcag tttgaagttg
    16921 cacgccaaat ggctgtagca tttgccggac tgaaaacgcg ctacccgcat atcgacacgc
    16981 tctctctggg aatgtcggac gatatggaag ccgccattgc ggcaggtagc acgatggttc
    17041 gtatcggcac tgcaattttt ggtgcgcgtg attactctaa aaaataagga attaaaggaa
    17101 cgccatgaat acgttgactt tcctgctttc aacggtcatt gagctgtata ccatggtgct
    17161 gttattacgc atctggatgc agtgggctca ttgtgatttt tacaacccct tctcacagtt
    17221 tgtagtgaag gtaacgcagc caattatcgg gccactgcgc cgcgttattc cggcaatggg
    17281 tcccattgac agcgcctcgc tgctggttgc ctatattctc agttttatca aagccattgt
    17341 gctgtttaaa gtggtgacct tcctgccaat catctggatt gccggtttac tgattctgct
    17401 gaaaaccatc ggcttgctga ttttctgggt cctgctggtg atggcgatta tgagctgggt
    17461 aagccagggt cgtagtccga ttgaatacgt gctgattcag ctggccgatc cgctgctgcg
    17521 cccgattcgc cgcctgctac cggcaatggg tgggattgat ttctcgccga tgatcctcgt
    17581 tctgctgctg tatgtcatca atatgggtgt cgcagaagta ttacaggcaa ccggaaacat
    17641 gctgctgccg gggctgtgga tggcgttatg agtgccgtaa cagttaatga tgacggtctg
    17701 gttttacggc tctatattca gccgaaagcc agccgtgatt ctattgtcgg tttacatggc
    17761 gacgaagtta aagtcgccat taccgcgccg ccggttgacg gccaggccaa cagtcatctg
    17821 gtgaagtttc tcggtaagca attccgggtt gccaaaagcc aggtggtgat tgaaaaaggc
    17881 gaacttggcc gccacaaaca aattaaaatc attaatccgc aacaaatccc gccagaaatc
    17941 gcggcgttaa ttaattaggt atcctatgca aaaagttgtc ctcgcaaccg gcaatgccgg
    18001 taaagtgcgt gagctggcgt cgctgcttag cgacttcggt cttgatatcg tggcccaaac
    18061 agacctcggc gtagattccg ctgaagaaac cggcctgacc tttatcgaaa acgcgattct
    18121 gaaagcgcgc catgcggcaa aagtgaccgg tttaccggca attgccgacg actctggtct
    18181 ggcggtagat gtgcttggcg gcgcgccggg catttactcc gcgcgttatt ccggtgaaga
    18241 cgcgaccgat ctaaagaatc tgcaaaaact actggaaaca ctgaaagacg taccggacga
    18301 ccaacgtcag gcgcgtttcc actgcgtgct ggtatatctg cgtcacgcgg aagatccgac
    18361 tccgctggtg tgccacggta gctggccggg cgtgattact cgtgaaccag cgggcactgg
    18421 tggctttggt tatgatccaa tcttcttcgt accttccgaa gggaaaaccg ctgccgaact
    18481 gacccgcgaa gaaaagagcg ccatttccca ccgtggtcag gcattgaaac tgctgttgga
    18541 cgctttacgt aatggttaaa ttaccgccgc tgagtctcta cattcacatc ccgtggtgcg
    18601 tgcagaaatg cccgtactgc gatttcaact ctcacgcgtt gaaaggagaa gtgccgcacg
    18661 acgattacgt tcagcatctg ctttgcgatc tggacaatga tgtggcttac gctcagggcc
    18721 gtgaagtaaa gacaatcttt attggcggtg gtacgccgag cctgctttcc ggcccggcga
    18781 tgcaaacgct gctggacggc gtgcgtgcgc gtttgccgct ggcagcggat gcagaaatta
    18841 ctatggaagc gaaccctggt acggtagaag ccgatcgctt tgtcgattat cagcgtgctg
    18901 gtgtgaaccg catctctatt ggtgtgcaga gttttagcga agaaaagctg aaacgacttg
    18961 ggcgtattca tggcccgcaa gaagcgaaac gcgcggcaaa gctggcgagc gggctggggc
    19021 tgcgtagctt taacctcgat ttgatgcatg gactaccgga tcaatcactg gaagaggcgc
    19081 ttggcgatct acgccaggcc attgaactga atccgccgca tctttcctgg tatcaactga
    19141 ccatcgaacc taatacgctg tttggttcac gcccgccggt actgccggac gacgacgcgc
    19201 tgtgggatat attcgaacag gggcatcagt tattaaccgc agcgggttat cagcaatatg
    19261 aaacttccgc ttacgccaaa cccagttatc agtgccagca caatctcaac tactggcgct
    19321 ttggcgacta catcggtatt ggctgcggcg cgcacggcaa agtgaccttc ccggatgggc
    19381 gcattctgcg taccactaaa acgcgtcatc cgcgtggttt tatgcaagga aggtatctgg
    19441 aaagccagcg tgatgtcgaa gccgcagata agccgtttga gttctttatg aaccgctttc
    19501 gcttgctgga ggccgcgcca cgcgcagagt ttagtgcgta taccgggctt tgcgaagatg
    19561 tgattcgccc acagttagac gaggcgattg cccagggtta tctcaccgaa tgtgcggatt
    19621 actggcagat aacggaacat gggaagctgt ttttaaattc gctgctggag ctttttctgg
    19681 ctgagtaaac ttgtattgcc ggatgcggcg tgaacgcctt atccagccga catgtggcag
    19741 cggttgtagg tctgataaga cgcgcaagcg tcgcatcaga cgttgattgc cggatgcggg
    19801 gtcaacgccg catcctgcta caaatcgtgc actatatcaa acttacttaa tcaccccatt
    19861 gcgccgcagt tgttcggtat cttttttcca gcgttcatta gcttggttga tatcgtcaat
    19921 tttcatcaaa atcgcgactt ttttactgtt tatttctgaa actttcatcg ttaacggata
    19981 ttgccctttt tcgagcgtta cccagtcatc ctgcaattta cggctgagtt tttgtgattg
    20041 cagaaaagtc tgtcgccgtt gttcgttgat atcgaaatct cgtttctgct gaatcggcac
    20101 ttcataacac tcttcactct gtttatgaca tgccgcaatc gccggttgat aaacctcttt
    20161 gtcatattta accgcgaacg cctcgctatc gttctgttta ttaaactcat cacgctgttg
    20221 gtgaattttt ttaaaggctt cttcgcgggt catttgctta ccgtttgcat cttttcccca
    20281 atatgcatcc gctgctttct gcgccgccac catttgctta tcaagcgctt tcacttcctt
    20341 ttccagcgca ggaatcgagt tcttctgggt attcaacgca gattcaagct gactaaagcg
    20401 ataattgaaa tcttcgctat tcacgatcag atatttatta ttcgttttga tatcgtcgac
    20461 cacccgccca cgatcgctgg ctgccgcctg aaaagagtaa aagttcaccg accagccaga
    20521 cgcgggcgtt cctttactgg ttaacatcgc cgagaatttt accggtttat ctttcgtcca
    20581 ggtctgttcg agcagttcgt aatctgccaa ctgaccgacc caggtataga gatcgtcact
    20641 ggaagagaca tcgccttccg ctgaccaggt ggcctgatta cccttagcat caagattttt
    20701 taaggtgata gcatcaagct tgagaatgcc gtgatactgc tttttgaatt gatcttttaa
    20761 gatattttca gttggcggtt cgccatctgc ccaggcatta ccagtcatca acataagcag
    20821 tgccgtgcca acaatccatt gctttttcac ccgaaaaacc tcacgcggaa taatttattc
    20881 cggtgattat cattagggta atgaaaattg gcaatggacg taagtctgaa gtgaaaaagc
    20941 cccggcacga taccggggcg aggtgattag tactgattga agatctgctg gatctgctgc
    21001 ggatctttgg tttgcgtcag agccagttgc agcagaacgc gcgctttttg cgggttcagc
    21061 gtgccagagg cgacgaagcc gtatttcgca tcatccactt cggcatcctg agtggtagcg
    21121 cccgtcggta cgcgggaaga acgcacgact gcagtaccgt ttttcgcggc ggtcgccagc
    21181 gtgtcgaaca cagatttata caggttgccg ttacccacac cagcgctaac gatgccatca
    21241 tagcccgcat ctaccagtgc tttagccgga agatcggatg cgttagcgta gttataaaca
    21301 atgccgactt tcggcagttc attcagctta gagacatcga atggcgtgtc gctggtatgc
    21361 ttacgtgccg gggtacgctg gtagtcaatc ttaccgttgt gaatgtaacc cagaggaccg
    21421 tagttaacag acttgaaggt cgctacgtcg gtggtgttgg ttttggtgac gtcacggcca
    21481 tcaagcacgg tgtcattcat cactaccagc acgccacggt tggcggaggc tttatcagct
    21541 gcggtcacta ccgcgttata caggttgaat ggaccgtctg cgctcataga cgtggacgga
    21601 cgcattgcgc cgaccatcac caccggtttg tcgcatttca ccgtcaggtc gaggaagtaa
    21661 gcagtttctt ccatcgtgtc ggtaccgtgg gtaatgacga agccgtcggt cttatcgcag
    21721 tcggtgttaa tttttttcgc cagtgtcagc cagacattat cgttcatgtc ctgggagccg
    21781 atattcacta cctgctcgcc tttaacgttc gcaatgtcct tcagttgcgg caccgcatta
    21841 accagatttt ctacgccaac tttacccgct gtgtagttag atttggttgc ggagtcacca
    21901 ccaccggcaa tggtcccgcc ggttgctaaa atggtgatat tgggtaatgc caatgctgca
    21961 ccactaaaac ccataaccag tgcggcaagt gccgtctttt tgaaaaactc catttcattc
    22021 ctccagttac gtgaacgcta cgcattatcc cttagctctg tatgggaaat ttgacgttaa
    22081 acaatttaca acgtgaatat attttggaga tctacaaagt tagaggcagg taacaaaacg
    22141 aagaattaaa cggcataaaa aagtattatg ccgtcttaaa atagaggatt attttaaatt
    22201 cccgaccagg gctttgcggc tatcttccag agtcacaacg cggctacaaa catctttgcc
    22261 aaactgctgg aaatcttttt cctgcttttt ccactcggtt tggattgagg attgcagccc
    22321 ccccaggctt cccagcacat tctgtaatgg gttaccgccg cttttcagca ccgctttcgc
    22381 gcccatttca ttaatgctgt cctgtaaaat tccacccatt gcctgattca ctaattgctg
    22441 gccttcggcg cgaacctgat caatggcttt atagtgaaac gtcaggccat cgctgcgcgt
    22501 ttcgataatg cggttcatct gctctttcag ctgcgcatca agtttggtca gacggctgcg
    22561 cattttgctg ctttcgccca tctcctgaac gataatttta tccagcgcaa tacgggcttt
    22621 ttcgacgcgg cttttcgcgc cttcatcaat ccacggcagc gtgctgcgta gttcagcctg
    22681 ataatccttc gcctgctcgc gctgggcggc attcagggaa tattgcttac cgttatacat
    22741 cacgttgccg tctggcgtga tcaccagatt gccgttttcg cccttcacct gcacggtttg
    22801 cgggctgaca atcacatcgt cacgcggcgt gacgctgcac tggtagtcgg cgtgagcggt
    22861 cattgccgtc actgaaagtg ctgccgccag cagcattttg cgcatcatag tcttccctca
    22921 agaaaaaatc aggccagcat ttgctggccc cagattgata acaaagtacg cgttttccat
    22981 gccggatgcg gcgtaaacac cttatcccgc ctactcgatc gtgcatcttt ccaggcctga
    23041 taagcgtagc gcatcaggca gttttgcatt tgtcatcgcc cgtatgcttt cttagtccca
    23101 ccaaacgtcg aaaagttcgc tggtgcgtac ctcatccagt ttgcgctctt ccagccactt
    23161 acgcacaatc gcctgatgtt cttcggtgca tttgccgatt tcctgcatgc agatcagacc
    23221 ttcccaggcc agataaccgc tgccgtcaaa ggccagtttg ttcggttcga taacctcgtt
    23281 aataaaatca tcaacggttt tatcaatctg ttcttccgat gtaccttccg ggaatcgcca
    23341 tgccaccgaa aatcctaatt cctggaattc gtcgatgtgc atttttttac gcagacgacg
    23401 gctacggttc tttgccatta tttcaccctc tcgaacatta agtcccatac tccgtgacca
    23461 agacgatgac cacgttgttc aaatttcgtc accggacgtg atgccggacg cggtacgtaa
    23521 tcattgctct ctgacaggtt tttataaccg tcaatagaag acatcacttc aagcatatgt
    23581 tccgcataag gttcccagtc ggtcgccata tggaataccc cccccagctg cagtttgctt
    23641 tttaccagtt cggcaaacgg cacctgtacg atacggcgtt tattatggcg cgctttgtgc
    23701 cacgggtcag ggaaaaagag ctgcaccatg cgcaatgaat tgtcaggaat cattttatgc
    23761 agcacttcaa ccgcatcgtg acacatcact cgcaggttgc ttaaaccttc ttcatgcgca
    23821 gaagccaggc acgcaccaac gcccggtgaa tgcacttcaa tgccgaggaa gtcctgctcg
    23881 gggcgatctt tagccattgc caccagcgat gcccccatgc caaaaccaat ctcaagcgtc
    23941 accggcgctt cacggccaaa aagcgcgggg aaatccagca tatcttcgct gaactcaacg
    24001 cccatcaccg gccagtagtt ttccagcgca tgttcctggc ctttggtcag tcgcccctgg
    24061 cggcgcacaa aactacggat acggcgcagt gggcggccgt tttcatcaaa ttccggtgaa
    24121 atgacgtcgt ttttcataaa gatttagtcg cttgtgaaag tgttctgaaa acgggcatta
    24181 tccaaagtta gttgccggat gcaagcatga taaggccgtg gctgcggaaa gttccggttt
    24241 acaccctgcc gtcgctgtgc tgcaatcttg cccccaacaa cagtgaattc ggtgaccatg
    24301 caagcgtcgc aattttcagc ccaggttctg gactggtacg ataaatacgg gcggaaaacg
    24361 ctgccctggc aaattgacaa gacgccctac aaagtatggc tctcagaagt gatgttgcaa
    24421 caaactcagg ttgcgaccgt tatcccctat tttgaacgct ttatggcgcg cttcccgacg
    24481 gtgaccgatc tcgccaatgc gccgctcgac gaagttctcc acttgtggac cgggcttggc
    24541 tattacgccc gcgcgcgcaa cctgcataaa gcggcacaac aagtggcgac cttacacggc
    24601 ggtaaattcc cggaaacctt tgaagaagtc gcggcgttac cgggcgtcgg gcgttccacc
    24661 gcaggtgcga ttctctcgct ttctctgggt aagcactttc cgattctcga cggtaacgtc
    24721 aaacgggtgc tggcgcgctg ctatgctgta agcggctggc cagggaaaaa agaggtcgag
    24781 aataaattat ggagtttgag cgagcaggtg acgcccgcgg ttggcgtgga acggtttaat
    24841 caggcgatga tggatttggg tgcgatgatt tgtacgcgct cgaaaccgaa atgttcgctc
    24901 tgtccgctac aaaacggatg tattgccgcc gccaacaata gctggtcgct ttatccgggc
    24961 aaaaaaccga aacagacgct gccggagcgc accggctact ttctcctgtt acagcacgaa
    25021 gatgaagtat tgctggcgca gcgtccgccg agcggattgt ggggcggttt atactgtttc
    25081 ccgcagtttg ccgacgaaga aagtttgcgg cagtggctgg cgcaacggca gattgctgcc
    25141 gataacctga cgcagctgac cgcgtttcgg cataccttca gccatttcca cttagatatt
    25201 gtgcctatgt ggcttcccgt gtcgtcattc accggctgca tggatgaagg caatgcgctc
    25261 tggtataact tagcgcaacc gccgtcagtt ggcctggcgg ctcccgtgga gcgtttgtta
    25321 cagcagttac gcactggcgc gccggtttag cgcgtgagtc gataaagagg atgatttatg
    25381 agcagaacga ttttttgtac tttcctgcaa cgtgaagcag aaggtcagga ttttcagctg
    25441 taccccggcg agctgggaaa acgcatctat aacgagatct ccaaagaagc ctgggcgcag
    25501 tggcagcaca aacaaaccat gctgattaat gaaaagaaac tcaacatgat gaatgccgag
    25561 caccgcaagc tgctggagca ggagatggtc aacttcctgt tcgagggtaa agaggtgcat
    25621 atcgagggct atacgccgga agataaaaaa taaaaacagt gccggagcac gcctccggca
    25681 acttgcataa aaacaaacac aacacgcacc cggaatgatg aaaaaatatc tcgcgctggc
    25741 tttgattgcg ccgttgctca tctcctgttc gacgaccaaa aaaggcgata cctataacga
    25801 agcctgggtc aaagatacca acggttttga tattctgatg gggcaatttg cccacaatat
    25861 tgagaacatc tggggattca aagaggtggt gatcgccggt cctaaggact acgtgaaata
    25921 caccgatcaa tatcagaccc gcagccacat caacttcgat gacggtacga ttactatcga
    25981 aactatcgcc gggacagaac ctgccgcgca tttgcgccgg gcaattatca aaacgttgct
    26041 gatgggtgac gatccgagtt cggtcgatct ctattccgac gttgatgata ttacgatttc
    26101 gaaagaacct ttcctttacg gtcaggtggt ggacaacacc gggcagccga ttcgctggga
    26161 aggtcgcgcg agcaacttcg cggattatct gctgaaaaac cgtctgaaga gccgcagcaa
    26221 cgggctgcgt atcatctaca gcgttaccat taacatggtg ccgaaccacc ttgataaacg
    26281 tgcgcacaaa tatctcggca tggtccgcca ggcgtcacgg aaatatggcg ttgatgagtc
    26341 gctgattctg gcgattatgc agaccgaatc ttcctttaac ccatatgcgg tcagccgttc
    26401 cgatgcgctg ggattaatgc aggtggtaca acatactgcc gggaaagatg tgttccgctc
    26461 gcaggggaaa tccggcacgc cgagccgcag tttcttgttt gatcctgcca gcaatattga
    26521 taccggcacc gcgtatctgg cgatgctgaa caatgtttat ctcggcggaa ttgataaccc
    26581 aacatcgcgg cgttatgccg tcatcaccgc ctataacggc ggcgcaggca gcgtgctgcg
    26641 agtcttttcg aatgataaga ttcaggctgc caatattatt aacaccatga cgccgggcga
    26701 tgtttatcag acgctgacga cccgccatcc ctctgcggaa tctcgccgtt atctttataa
    26761 agtgaatacc gcgcaaaaat cctaccgccg ccgataattc cattaaccgc ccctgacgat
    26821 gctcaggggc aaaaatgtta tccacatcac aatttcgttt tgcaaattgg gaatgtttgc
    26881 aattatttgc cacaggtaac aaaaaaccag tccgcgaagt tgatagaatc ccatcatctc
    26941 gcacggtcaa atgtgctttt tcaaacactc atccgcatca cgatgtgagg aaattaacat
    27001 gaatcttaag ctgcagctga aaatcctctc ttttctgcag ttctgtctgt ggggaagttg
    27061 gctgacgacc ctcggctcct atatgtttgt taccctgaag tttgacggtg cttctattgg
    27121 cgcagtttat agctcactgg gtatcgccgc ggtatttatg cctgcgctgc tggggattgt
    27181 ggccgacaaa tggttaagtg cgaaatgggt atatgccatt tgccacacca ttggcgctat
    27241 cacgctgttc atggcggcac aggtcacgac accggaggcg atgttccttg tgatattgat
    27301 taactcgttt gcttatatgc caacgcttgg gttaatcaac accatctctt actatcgcct
    27361 gcaaaatgcc gggatggata tcgttactga cttcccgcca atccgtatct ggggcaccat
    27421 cggctttatc atggcaatgt gggtggtgag cctgtctggc ttcgaattaa gccacatgca
    27481 gctgtatatt ggcgcagcac tttccgccat tctggttctg tttaccctga ctctgccgca
    27541 tattccggtt gctaaacagc aagcgaatca gagctggaca accctgctgg gcctcgatgc
    27601 attcgcgctg tttaaaaaca agcgtatggc aatcttcttt atcttctcaa tgctgctggg
    27661 cgcggaactg cagattacca acatgttcgg taataccttc ctgcacagct tcgacaaaga
    27721 tccgatgttt gccagcagct ttattgtgca gcatgcgtca atcatcatgt cgatttcgca
    27781 gatctctgaa accctgttca ttctgaccat cccgttcttc ttaagccgct acggtattaa
    27841 gaacgtaatg atgatcagta ttgtggcgtg gatcctgcgt tttgcgctgt ttgcttacgg
    27901 cgacccgact ccgttcggta ctgtactgct ggtactgtcg atgatcgttt acggttgcgc
    27961 attcgacttc ttcaacatct ctggttcggt gtttgtcgaa aaagaagtta gcccggcaat
    28021 tcgcgccagt gcacaaggga tgttcctgat gatgactaac ggcttcggct gtatcctcgg
    28081 cggcatcgtg agcggtaaag ttgttgagat gtacacccaa aacggcatta ccgactggca
    28141 gaccgtatgg ttgattttcg ctggttactc cgtggttctg gccttcgcgt tcatggcgat
    28201 gttcaaatat aaacacgttc gtgtcccgac aggcacacag acggttagcc actaattacg
    28261 caaagaaaaa cgggtcgcca gaaggtgacc cgtttttttt attcttactt caacacataa
    28321 ccgtacaacc gtttcatgcc atccgcatcg gtttcgctat aaacaccttg cagctccggc
    28381 gaaaatcccg gcaacaaatt caccccttct tccagtgcaa ggaaataacg ttgaaccgcc
    28441 ccaccccaga cttccccggg taccacgcaa agcacgccag gtggataagg caacgcccct
    28501 tctgccgcaa ttcgcccttc ggcatcacga atccgcacca actccacttc accgcgaata
    28561 taagcgctat gcgcatcctg ggggttcatc accactgacg ggaaactctg ctggcggaac
    28621 atcgcttttt gtaggtcttt gacgtcgaaa ctgacataca gatcgtgcat ctcctgacac
    28681 aactggcgca tggtgtagtc gcgatagcgc accggatatt tgttataaac gctcggcaac
    28741 acctcagcca gcggcgagtc atcctcaata tgctgttcaa attgcgccag catcgccacc
    28801 agttgtgcca gcttctcgtg gctttccgcc ggagttaata agaacagaat ggagttgaga
    28861 tcgcacttct ccggcacaat gccgttctca cgcagatagt gcgccagaat cgtcgccgga
    28921 acgccaaagt cgctatattc gccagtttcg gcattgatac ctggtgtagt gagtaacagc
    28981 ttgcacggtt caacaaaata ctgatccgcg gcatatcctt caaagccgtg ccacttcgcc
    29041 cccggctcaa aactgaaaaa acggcggtcg ctggctaaca cagatgtcgg ataatcctgc
    29101 cacaatttgc catcaacaac gggcgggata aacgggcgga acagcttaca gcgcgcaaga
    29161 atagccttgc gcgactcaat acccaactca acgcactcag cccacagccg acgcccactc
    29221 tctccttcat gaattttagc gttaacgtcc agcgcggcaa acagcgggta gaacgggctg
    29281 gtagaagcat ggagcataaa ggcattattc aaccgcttat gcgggcaaaa acgcgcctgt
    29341 ccgcggatat ggttatcttt tttatggatc tgcgacgtct gtgagaatcc cgcctgctgt
    29401 ttgtgcaccg actgagtcac aaagatcccc ggatcgtttt cgttaagttc taacagcagc
    29461 ggcgagctat ccgccatcat cgggataaat tgttcataac cgacccacgc ggaatcaaac
    29521 agaatgtaat cacacagatg cccaacggta tcgatcacct gacgggcgtt atagacagtg
    29581 ccgtcatagg ttcccagctg aataatcgcc aggcgaaacg ggcgtggcaa ctcagctttt
    29641 tctggcgcaa cgtcgcgaat ttgctggcgc agatactctt cattaaaaca gtgcgcatca
    29701 ataccgccaa tgaaaccaaa cgggttgcgt gaagcttcca gatagaccgg cgtcgccccc
    29761 gcctgaatca gcgcgccgtg atgattcgac ttatggttgt tacggtcgaa gagcaccaga
    29821 tcgccacgcg ttaacagcgc attcgtcacc actttattcg ctgccgatgt gccgttcagc
    29881 acaaaatagg ttttatcggc atgaaagact ttggctgcga atttctgcgc atctttcgcc
    29941 gatccttcat gaataagcag atcgcccaat tttacgtcag cgttacacat atcggcgcga
    30001 aagacgttct caccaaagaa atcgtaaaaa tggcgtccgg caggatgctt tttaaaaaac
    30061 gcaccatgtt gatgtccagg gcaagcaaag gtgctgttgc ccatctcaac gtactgcgtc
    30121 agcgtgtcat aaaacggtgg cagcaaattc tcttcatact gacaggctgc ggattccagc
    30181 tccagccatt gctgctcgtt gccgttgatt accgccgtaa cgcccgcagg taattcaaca
    30241 gcatgttcgg aatacaaaaa caccggtaga tgaaaaccgg tgcgcttaag caacgcaaga
    30301 atgccactgc gactatccgc agcggtaatg acgactgccg cgacgtccgt aaaatcagta
    30361 tctcccaacg ccaccacgcg acgatgagaa gaaagtcggg ataccagatc actactggcg
    30421 gcaatattca ttgatttcat aagcgcaaac ccgtttcggg gaagtaagaa taccggacaa
    30481 ggtggaaaac cctgccccat gagatatggg tcaaactggt caccagctcc gaccgccaga
    30541 catcagtaaa agcagaaacg ctctgatttt actgttgtcc tgcagtgagc gtgcgttaac
    30601 ttcaccgcat gagcagtaac atagaaaggg aaacgtttcg cgcgaaacgg cgataagcga
    30661 gagaatgtaa ggaggtggcg tgcatcgggc aaactccgta cagagaggag aaaattcgcg
    30721 caatcatggc acctttcgct aaagcgtgca agccaggact ttgcgaacaa acaagccatc
    30781 gacggcacca aacggtcata ataagaaaat caaacaatac agttaacagg aactcttgtg
    30841 gtcatcggcc cttttatcaa cgctagtgca gtattactgg gtggcgttct cggcgcactg
    30901 ctcagccaac gcttaccgga acgtatccgc gtctccatga catctatttt tggtctggca
    30961 tcgctgggga ttggtatttt actggtggtg aaatgtgcca accttccagc gatggtttta
    31021 gctacgctac ttggcgctct aattggcgaa atttgtttgc tggagaaagg tgtcaataca
    31081 gcagtcacca aagcacaaaa tctgtttcgt cactcacgta agaagccagc gcatgaatct
    31141 tttattcaga attatgtcgc gattattgtc ctgttttgcg ccagcggcac cgggatcttc
    31201 ggggcgatga acgaagggat gaccggcgat ccgagtattt taatcgccaa gtcatttctt
    31261 gatttcttta cggcgatgat cttcgcctgc tcgctgggta ttgcggtgtc ggtaattagt
    31321 atcccattac tgatcatcca gttaacgctg gcgtgggctg ccgcgctgat attaccgctg
    31381 accacaccgt cgatgatggc agacttcagc gccgtaggcg gtttattgct gctggcaacc
    31441 ggattacgca tctgtggcat taaaatgttc ccggtggtca acatgcttcc agcactcctt
    31501 ctggcaatgc cgctttccgc cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt
    31561 gatgatagat tgtgcagtct gcagtaaatt gaagaaattt gattgacgag acgaggcgaa
    31621 tcaggtttaa tgcgccccgt tgcccggata gctcagtcgg tagagcaggg gattgaaaat
    31681 ccccgtgtcc ttggttcgat tccgagtccg ggcaccactt tcacttccag attcatctcc
    31741 cgaactccac gaaactcaag tataatcaca tcgttacaag caatctgaac attctgaact
    31801 ccaccagact ctactgaact ctctctcaat caagcgttca ttgggggccc cggtgggggc
    31861 tcattgtgtt cagtcagttt caggagcccc catatggcac ttacagccag acaggtcgaa
    31921 acagccagac ctaaagaaaa agactataaa ctctctgacg aacgtggttt atatctgctg
    31981 gtaaaaacca cgggtgcccg ctactggcgg cttaaatacc ggatagcagg aaaagagaaa
    32041 aaactggccc tcggcgtcta tcccgacgtc tcccttgctg aggccagaat caaacgcgac
    32101 gatgcccgaa aaatcatctc cgaaggtggt gacccgggcg aaaagaagcg aaaggaaaaa
    32161 ctcactcaga aaatctctgc caccaatacg ttccatgccc tcgctacgga atggcaccag
    32221 cataaatctt tgtcatggtc tgaaagttac gccagaagcg tactggaagc gctggataaa
    32281 gatattttcc cgtatctggg caaacgaagc gttacggata tcctcccgct ggaaatgctg
    32341 gaaattctgc gccgcataga aaaacgtggc tcgctggaaa aacttcgtaa ggtgcgtcaa
    32401 tactgtaatc agatttttcg ttatgccatc gccaccggac gagccactgt caatccggca
    32461 tctgaactga ccagtacgct ggcggcgcca aaagctgcac atttccccca cctgagagca
    32521 gatgagctcc ctgtttttct ccggaagctc gctgagtatc atggcagtcc tgttacccgc
    32581 atggcgacaa atctgctgct tctgacaggt ctcagaacca ttgagctacg gtccgctgaa
    32641 tggtcagaaa ttgattttga taatgcactg tggacaatcc ctgaaagccg catgaaaatg
    32701 cgacgtaaac atgtcgtacc actgtcacga caggccactg acattctgct gcagctcaaa
    32761 actttctccg ggcaataccg gctggttttt ccgggacgtt gtgatatcaa caagccaatg
    32821 agcgaagcca gcatcaatat ggtgctcaaa cgtatcggtt acgatggcag ggcaaccggt
    32881 catggttttc gtcacaccat gagtaccatt ctgcacgaac agggctttaa ttctgcctgg
    32941 attgaaatgc agttagctca tgtggataaa aattccatca ggggtaccta taatcatgcc
    33001 ctgtatctcg atggtcgccg tgaaatgatg caatggtacg ctgactacat tgattcactt
    33061 tccatccagg agagttaatt acactttgtc ctgaaattat tctcacaatc aataaccttt
    33121 ccctctgatc tgtaagatca gagggattcc gttaacttac aataagtgat attggttgtc
    33181 tggagattta gggggacggt ctactataat aattagcagg agataatatg tcacttaagg
    33241 cgattgccac tgcactcgga gtttctgtca ccactgtcag tcgggctctt ggaggctatg
    33301 cagatgtgtc agcttccacc cgtgcacggg tggaagctga agctcgtcga cgcggttacc
    33361 gtcctaatac tcaggcaaga agactcaaaa ccggtaaaac cgatgctgtc ggtctggttt
    33421 atcctggacg tgatgtgcca tttaacagtg gtgtttttat ggatatggtc agttgcatca
    33481 gccgggaact tgctcaccat gatattgact tactactgat agctgatgat gagcatgccg
    33541 attgccacag ctatatgcgg cttgttgaaa gtcgcagggt tgatgctctc atcattgcac
    33601 acactctgga tgacgatccc cgtatcacac accttcataa agcaggtatt ccatttctgg
    33661 ctcttggtcg ggtaccatct ggtctgccct gtgcgtggtt tgactttgat aatcatgccg
    33721 ggactggaca ggcaacccag aaactgattg ctttgggaca taaacgtatt gcattgttga
    33781 gtgaagacac ttcacatagc tatgttgttg caagacgtca gggatggctt gatgcactga
    33841 atgagcatga actggaagat acatggctgc ggctggtttc tcccacacgg cgagcaggat
    33901 atcaggccgt gatggagtta atgtcactac cgataccacc aacagctatt attactgaca
    33961 atgacctgag tggagatggt gctgccatgg cactgcagtt gagcggtcgt ctttcaggaa
    34021 aagatgcggt atctctggtt gtatatgatg gtttgccaca ggacagtatt attgagctgg
    34081 atgtggctgc agttattcag tcaacacgaa gcctcgttgg tcgccagatt tccgagatga
    34141 tatatcagat aattactgat tcatcatcaa aaccactaca ggttgtctgg aacccggtat
    34201 tttatccggg aaagacgatt catcctcctt gctcctgatt tttttatcca gctcacatat
    34261 ccgaaacgtt ttggttgata cccgaaacgt ttcggatcaa cagtaaggca tccctgagaa
    34321 aggagaatgt cttatggttt caaagtactg cagactgagc agtcctcgct ctgatttaat
    34381 tattaaaacc cgtccacatg cagaaattat ctggtggggc tctgcactga aacatttctc
    34441 accggatgac tgtgccagcc tggaaagacc ggttgcgaat ggtcgtctgg atattgatac
    34501 gccactaaca ctgatggctg aaaatgccct gggacttttt agctctccgg gactggaagg
    34561 acacaggaat gggctggatg catctcctgt tttttataca gttgacgtgg aacataccga
    34621 aaacactctg agacttacca gtgaagattc ggtagccggc ctgcgtctgg tcagcgagct
    34681 ggtgatgacg ccatcaggaa ttctgaaagt tcgtcatgca ctgaccaacc tcagagaggg
    34741 ggactggcag ataaatcgtt tcgccatcac tttaccttta gcggaacgtg cggaagaagt
    34801 catggctttt cacggacgct ggactcgtga atttcagccg cacagggtac gtctcactca
    34861 tgatgctttt gttctggaaa atcgcagagg gcggacatct catgagcatt ttccggcgct
    34921 gattgtcggc acgccaggct tctcggaaca acagggagag gtgtgggctg tgcatctggg
    34981 gtggagtgga aatcaccgca tgagatgtga ggcaaaaact gacggcaggc gttacgttca
    35041 ggctgaggct ctgtggatgc cgggtgagaa ggctctcagg aagaatgaaa ccctgtacac
    35101 cccgtggcta tatgcctgcc actctgcgga tggcctgaat ggaatgagtc agcaatacca
    35161 tcgttttttg cgtgatgaaa ttatccgttt ccctgagcaa aaaccccgcc ctgtacatct
    35221 caatacctgg gaaggtattt atttcaatca caatcctgat tacatcatgc agatggctga
    35281 gcgtgcagca gcactgggcg ttgaacgttt cattattgat gatggctggt ttaaaggacg
    35341 taacgatgac cgcgcggctc tgggcgactg gtataccgat gaacagaaat acccgaacgg
    35401 gctgatgccg gttattaaac atgtgaaatc tctcggtatg gaatttggca tatgggttga
    35461 gccggaaatg attaacccgg attctgacct gtttcgtctt catcctgact gggtattgtc
    35521 aatgcctgga tattcccagc caaccggaag atatcagtat gttcttaacc tgaatattcc
    35581 agaggccttt gcttacattt atgaacgttt cttatggtta ctgggagaac atccggttga
    35641 ttatgtgaaa tgggacatga atcgtgagct tgtacaggca gggcatgaag gccgtgcggc
    35701 agcagatgca cagacccgtc agttctatcg attgcttgat ctcctccgtg aacgttttcc
    35761 acatgttgag tttgagtcct gtgcttccgg tggggggcgt attgacttcg aagtcctgaa
    35821 acgcacacac cggttctggg catctgacaa taatgatgcc ctggagcgct gcaccataca
    35881 acgtggcatg agttactttt tccctcctga ggtgatgggg gcgcatattg gccatcgccg
    35941 ctgccatgca actttccggc agcacagcat cgcttttcgt gggctgacgg cattgttcgg
    36001 ccatatgggg ctggagctgg atccggtggc cgcagatgcg aaggaatctg acggttatcg
    36061 ccggtatgcc ttgctctata aagaatggcg acaactgatt catacaggag ttctctggcg
    36121 tgtggatatg ccagatcctt cgatacaggt tcagggagtc gtcagccctg atcagtctca
    36181 ggcacttttt atgatcagcc agcttgcaat gccggattac accttaccag gcatacttcg
    36241 ttttcccggt ctggcggcgg aagtgcgtta ccggcttcgg gttattgatc acccggacct
    36301 ccaggtggtt ggtgaaggcg gtcataccat gcgcaaatta cctgtctgga tgaatcagag
    36361 ccttgaggcc agtggtgaat ggctggcaca gggagggatt cagctccccg tactggatcc
    36421 ggagagtgcg attttgatag cacttgaaag agctgtgtga agagaggtac ggagcctcag
    36481 ctccgtcctg tttctcaata ttatcccgtg agggtaagaa taatgaattc tgcaagtacg
    36541 cataaaaatc ccgatttctg gattttcggt ctgttcttct ttctctactt tttcatcatg
    36601 gcaacctgtt ttccgttttt gcctgtatgg ctgtccgatg tggtcggact gagtaaaacg
    36661 gatacaggta tagtcttttc atgtctttct ctgtttgcca tcagtttcca gccattgctt
    36721 ggggtcatat cagatcgcct gggactgaaa aaaaacctga tctggagtat cagcctgtta
    36781 cttgtatttt tcgccccctt ttttttatat gtatttgcgc ccctgctgcg cttcaatatc
    36841 tgggcaggcg cactgactgg cggtgtcttt attggttttg tgttttctgc aggtgccgga
    36901 gctattgaag cttatataga gcgggtcagt cgcagtcgtg gatttgaata cggtaaagcg
    36961 aggatgttcg ggtgtctggg ctgggcgtta tgtgcggcta tggctggaat gctttttaat
    37021 gtcgatcctt ctctggtttt ctggatgggg tcaggaagcg cattattgtt gcttcttctg
    37081 ttgtttctgg cgcgccccag taccagccag acggcaatgg ttatgaatac actgggtgcc
    37141 aattcttccc tgatttcgac cagaatggtc ttcagcctgt ttcgcatgcg tcagatgtgg
    37201 atgtttgttc tctacacgat tggtgtggcc tgtgtctatg atgtatttga tcagcagttt
    37261 gccacatttt ttcgttcatt ctttgacact cctcaggcag gaataaaagc attcggattt
    37321 gctaccactg cgggggagat ttgtaatgcc attatcatgt tctgtacacc atggataatt
    37381 catcgcattg gtgccaaaaa taccctgctt gttgcggggg gaattatgac catccgcatt
    37441 accggttctg cttttgccac caccgcgaca gaagtggtga ttctgaaaat gcttcacgct
    37501 cttgaagttc catttttgct ggttggggcg ttcaaatata ttacggcagt gtttgacacc
    37561 cgactgtcag cgaccgttta tttaataggt tttcagtttt ccaaacaact tgctgcaata
    37621 cttctctcta cctttgccgg ccacctgtat gatcgtatgg gattccagaa tacgtatttt
    37681 gtgctcggga tgattgctct gactgttacc gtgatatcag ttttcacgct gagttcttcc
    37741 cgcgggagcg tacacccttc tgtagaaaaa gcccctgcag cgcattcgga gattaactga
    37801 tgaaacagcg tcttgctttg gcacagtctg cccttgaaaa actttgcgca cgtcgtggta
    37861 atgcctggta cccgattttt catctggctc cacctgccgg ctggatgaat gatccaaatg
    37921 gccttattta cttcaatggg cgttaccatg cgttcttcca gcatcatcct gcaagcgcat
    37981 atcaggggcc aatgcactgg gggcatgcca ccagtaccga catgttgcac tggcaacacg
    38041 aacctatcgc gctggcaccc ggagataaat atgatcgtga tggctgtttt tcagggagtg
    38101 ccgtggatga tgatggcgtg ctatcactta tttatactgg tcatatttgt ctcgatgatc
    38161 gtggtaatga cagcattatc cgtgaagtac agtgtctggc tacaagtcat gatggtattc
    38221 actttgagaa gcagggctgt gtgctgacac ctccggaagg tataatgcat ttccgtgatc
    38281 ccaaagtctg gcacgaagac ggctcctggt ggatggtcat tggtgcccgg gacgcttctg
    38341 acaatgggca agttctgttg tatcgcggga catctttgcg ggactggcat ctggagcatg
    38401 ttcttgctca ttccgcagcc ggaaaaagtt atatgtggga atgccccgat ttcttcaggt
    38461 gtggtaattt tcactggctg atgttctcac cacaggggat gcccccttcc ggttatcggt
    38521 tccgtaacct ttttcagagc ggtgtgttgg cagggagctg gaagcctggt tctgtctttg
    38581 cgctgaaagg gggatttgaa gagctggatt atggtcatga cttttatgct ccacagtcca
    38641 tgctggctga ggacggcagg cgtatcatta tggcatggat gaatatgtgg gattcacccg
    38701 tgcccacccg cagtgaagcc tgggcaggat gtctgacgct gcccagagag gtttttgagc
    38761 gcgatggccg gctgtgccag cgacctgtgc gtgaagtcga atctctgcgc aaaaaatgcc
    38821 agccattatc ccctgtaagg ttacagggtt tgcaattact gaccgaaaat gtacaggccg
    38881 cagaattatt ggtgacgtgg catacggttg acagtcatgc ggagcactat ggcgtccgcc
    38941 ttggagacgg tctgcgactt tatgtggata atcaggccgg gcgattggta ctgtggcgct
    39001 attaccctga ggaagggttg gatggttacc gcagtgttga acttcctgat acagaatatc
    39061 tgactcttcg tattttcttg gatcgttcat ctgttgaagt gtttgttaac gatggtgagg
    39121 caaccttatc aagtcgtatt tatccgcaag cggactcgag acaattatcg ttatatgccg
    39181 ctcatggcga tgcgatatta actgatggca ctttatggat gctgacctga gacatatcga
    39241 tatcagtatt ttttctgaca ttatctgtca ttactttgcc agtacacatt aattatcttt
    39301 gctgtgtgct tttggtaatg tgttgcgatt atatatacca gtctggtgaa gaatatgaat
    39361 ggtattttg
//
LOCUS       DAJOWB010000032        38915 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJOWB010000032 DAJOWB010000000
VERSION     DAJOWB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.032"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(70..261)
                     /locus_tag="OHO85_001838"
     CDS             complement(70..261)
                     /locus_tag="OHO85_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCQ2671217.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            279..431
                     /locus_tag="OHO85_001839"
     CDS             279..431
                     /locus_tag="OHO85_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2671218.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVDLIIYND
                     DVIMMN"
     gene            613..2856
                     /gene="pdeF"
                     /locus_tag="OHO85_001840"
     CDS             613..2856
                     /gene="pdeF"
                     /locus_tag="OHO85_001840"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCQ2671219.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2895..4436)
                     /gene="ppx"
                     /locus_tag="OHO85_001841"
     CDS             complement(2895..4436)
                     /gene="ppx"
                     /locus_tag="OHO85_001841"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCQ2671220.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4441..6507)
                     /gene="ppk1"
                     /locus_tag="OHO85_001842"
     CDS             complement(4441..6507)
                     /gene="ppk1"
                     /locus_tag="OHO85_001842"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCQ2671221.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6678..7316)
                     /gene="purN"
                     /locus_tag="OHO85_001843"
     CDS             complement(6678..7316)
                     /gene="purN"
                     /locus_tag="OHO85_001843"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCQ2671222.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7316..8353)
                     /gene="purM"
                     /locus_tag="OHO85_001844"
     CDS             complement(7316..8353)
                     /gene="purM"
                     /locus_tag="OHO85_001844"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCQ2671223.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8678..9304
                     /gene="upp"
                     /locus_tag="OHO85_001845"
     CDS             8678..9304
                     /gene="upp"
                     /locus_tag="OHO85_001845"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCQ2671224.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9390..10679
                     /gene="uraA"
                     /locus_tag="OHO85_001846"
     CDS             9390..10679
                     /gene="uraA"
                     /locus_tag="OHO85_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCQ2671225.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10729..11475
                     /gene="hda"
                     /locus_tag="OHO85_001847"
     CDS             10729..11475
                     /gene="hda"
                     /locus_tag="OHO85_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCQ2671226.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11613..11972)
                     /gene="arsC"
                     /locus_tag="OHO85_001848"
     CDS             complement(11613..11972)
                     /gene="arsC"
                     /locus_tag="OHO85_001848"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCQ2671227.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVESEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11993..13456)
                     /gene="bepA"
                     /locus_tag="OHO85_001849"
     CDS             complement(11993..13456)
                     /gene="bepA"
                     /locus_tag="OHO85_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCQ2671228.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13669..14730
                     /locus_tag="OHO85_001850"
     CDS             13669..14730
                     /locus_tag="OHO85_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCQ2671229.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14768..15616)
                     /locus_tag="OHO85_001851"
     CDS             complement(14768..15616)
                     /locus_tag="OHO85_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HCQ2671230.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHYIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(15638..17650)
                     /gene="hyfR"
                     /locus_tag="OHO85_001852"
     CDS             complement(15638..17650)
                     /gene="hyfR"
                     /locus_tag="OHO85_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCQ2671231.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNVYYRYHDDGSDVTATAEYQGEMIDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17680..18093)
                     /gene="hyfJ"
                     /locus_tag="OHO85_001853"
     CDS             complement(17680..18093)
                     /gene="hyfJ"
                     /locus_tag="OHO85_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCQ2671232.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQAPAIYLMARKIA"
     gene            complement(18086..18843)
                     /gene="hyfI"
                     /locus_tag="OHO85_001854"
                     /pseudo
     CDS             complement(18086..18843)
                     /gene="hyfI"
                     /locus_tag="OHO85_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(18840..19385)
                     /gene="hyfH"
                     /locus_tag="OHO85_001855"
     CDS             complement(18840..19385)
                     /gene="hyfH"
                     /locus_tag="OHO85_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCQ2671233.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAQQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(19395..21062)
                     /gene="hyfG"
                     /locus_tag="OHO85_001856"
     CDS             complement(19395..21062)
                     /gene="hyfG"
                     /locus_tag="OHO85_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCQ2671234.1"
                     /translation="MNVNSSSNCGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(21052..22638)
                     /gene="hyfF"
                     /locus_tag="OHO85_001857"
     CDS             complement(21052..22638)
                     /gene="hyfF"
                     /locus_tag="OHO85_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCQ2671235.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            complement(22643..23293)
                     /gene="hyfE"
                     /locus_tag="OHO85_001858"
     CDS             complement(22643..23293)
                     /gene="hyfE"
                     /locus_tag="OHO85_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCQ2671236.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(23305..24412)
                     /locus_tag="OHO85_001859"
                     /pseudo
     CDS             complement(23305..24412)
                     /locus_tag="OHO85_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(24424..24534)
                     /locus_tag="OHO85_001860"
     CDS             complement(24424..24534)
                     /locus_tag="OHO85_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCQ2671237.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFYSPH"
     gene            complement(24551..25495)
                     /gene="hyfC"
                     /locus_tag="OHO85_001861"
     CDS             complement(24551..25495)
                     /gene="hyfC"
                     /locus_tag="OHO85_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCQ2671238.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLTPLFTGISRQIRARMHSRRGP
                     GIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGGG
                     DLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIAG
                     STHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTEY
                     SGAGLALAKWGLGLKQVVMAALFVALFLLFGRAQELSLACLLTSLVVTLLKVLLIFVL
                     ASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25506..27523)
                     /gene="hyfB"
                     /locus_tag="OHO85_001862"
                     /pseudo
     CDS             complement(25506..27523)
                     /gene="hyfB"
                     /locus_tag="OHO85_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(27523..28140)
                     /gene="hyfA"
                     /locus_tag="OHO85_001863"
     CDS             complement(27523..28140)
                     /gene="hyfA"
                     /locus_tag="OHO85_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCQ2671239.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVLPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(28393..28863)
                     /gene="bcp"
                     /locus_tag="OHO85_001864"
     CDS             complement(28393..28863)
                     /gene="bcp"
                     /locus_tag="OHO85_001864"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCQ2671240.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28863..29435)
                     /locus_tag="OHO85_001865"
     CDS             complement(28863..29435)
                     /locus_tag="OHO85_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCQ2671241.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29581..30459
                     /gene="dapA"
                     /locus_tag="OHO85_001866"
     CDS             29581..30459
                     /gene="dapA"
                     /locus_tag="OHO85_001866"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCQ2671242.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30476..31510
                     /gene="bamC"
                     /locus_tag="OHO85_001867"
     CDS             30476..31510
                     /gene="bamC"
                     /locus_tag="OHO85_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCQ2671243.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31723..32436
                     /gene="purC"
                     /locus_tag="OHO85_001868"
     CDS             31723..32436
                     /gene="purC"
                     /locus_tag="OHO85_001868"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCQ2671244.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32590..32817
                     /gene="vapB"
                     /locus_tag="OHO85_001869"
     CDS             32590..32817
                     /gene="vapB"
                     /locus_tag="OHO85_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCQ2671245.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            32817..33215
                     /gene="vapC"
                     /locus_tag="OHO85_001870"
     CDS             32817..33215
                     /gene="vapC"
                     /locus_tag="OHO85_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCQ2671246.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            33362..34225
                     /locus_tag="OHO85_001871"
     CDS             33362..34225
                     /locus_tag="OHO85_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCQ2671247.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            34240..36255
                     /gene="tmcA"
                     /locus_tag="OHO85_001872"
     CDS             34240..36255
                     /gene="tmcA"
                     /locus_tag="OHO85_001872"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCQ2671248.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            36329..37027
                     /gene="ypfH"
                     /locus_tag="OHO85_001873"
     CDS             36329..37027
                     /gene="ypfH"
                     /locus_tag="OHO85_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCQ2671249.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(37137..37337)
                     /locus_tag="OHO85_001874"
     CDS             complement(37137..37337)
                     /locus_tag="OHO85_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCQ2671250.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(37365..38492)
                     /gene="dapE"
                     /locus_tag="OHO85_001875"
     CDS             complement(37365..38492)
                     /gene="dapE"
                     /locus_tag="OHO85_001875"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCQ2671251.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38496..38852)
                     /locus_tag="OHO85_001876"
     CDS             complement(38496..38852)
                     /locus_tag="OHO85_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCQ2671252.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9492 a         9979 c        10229 g         9215 t
ORIGIN      
        1 aattccaaag agcaaaagca tccgacagcg aagtgccgtt aggttctgtg aaagtggaga
       61 aggtaaccgc taacgttgta ccggtgattc gacggagagt gtggattgct gagcttcttt
      121 tttgctctga tggtgctgcc aggcaccgac ggacgagtag ataaaacggc caaagaagaa
      181 gataaagctg atgagcagta cgatacgggt catgcgactg ttaaatcggt gtcgttttcg
      241 catactggtt gcctgactca caaaaggttc cttgaagtat gtcccacgct gtggacggta
      301 cttacattaa ggcacaacag gacaaaatgg tcaattcttc gttatgtaaa aaagcgtcag
      361 tggatacata ttttaatgtt atggaagttg atttaattat ttacaatgat gatgtaataa
      421 tgatgaatta acatgataaa cgtaataatt cattaatcag ggttattttg tttgatatat
      481 atcaattgga actttcatat gacacttaga atcaatgctc tctatagtga tgaataatca
      541 tcattcgaag tcaggtggga tgcctgtctg aatacacctc cttcaggatg tgggggattc
      601 tgctgagcat ctatgaaact gaatgcaact tatataaaaa tacgtgataa atggtggggg
      661 cttccgctgt tcctgccttc tttaatcttg cccattttcg cccacattaa tactttcgcg
      721 catatttctt ccggtgaggt ttttctcttt tatctgcctc tggcactgat gatcagcatg
      781 atgatgtttt tcagctgggc ggcattgcca gggatcgcct tagggatttt tgtccgcaaa
      841 tatgcagagc tgggttttta cgaaacgcta tcattaacgg ctaattttat tatcattatc
      901 attctctgtt ggggcggtta cagggtcttt actccccggc gtaacaacgt ttcacatggt
      961 gatacccgtt taatttccca gcgtatattc tggcagattg tgtttcctgc aacgctgttt
     1021 ctgatacttt tccagtttgc tgcatttgta ggattactgg cgagcagaga aaatctggtc
     1081 ggtgtcatgc cttttaacct cgggacttta atcaattatc aggccttact ggtgggtaat
     1141 ctgatcggtg tcccgctgtg ctacttcatc attcgggtag tgcgaaatcc attttattta
     1201 cgtagctatt attcgcaatt aaaacagcag gttgatgcca aagtcaccaa aaaagagttc
     1261 gcgctctggc tactggcatt aggtgcttta ttgttgctgt tatgcatgcc gttaaatgaa
     1321 aaaagcacaa tttttagcac caattatacc ttgtcattat tgctgcccct gatgatgtgg
     1381 ggagcgatgc gctatggtta taagctgatt tcgctgctct gggcggtcgt gttgatgatc
     1441 agcatccaca gctatcaaaa ttacattccc atttatcctg gctataccac gcagctgacc
     1501 ataacctcct ccagttatct ggtattctct tttattgtca attatatggc tgtactggca
     1561 actcgtcagc gagcggtagt cagacgcatt cagcggcttg cgtatgtgga cccggtggtt
     1621 catctgccaa atgttcgcgc cctgaatcgc gcgttgcgtg atgccccctg gtctgcgctt
     1681 tgttatttac gcatccctgg catggaaatg ctggttaaga actatggcat catgctgcgg
     1741 attcaataca agcaaaaact ttctcactgg ctgtcaccct tgctggaacc gggtgaagat
     1801 gtttatcagc tttcgggtaa cgatctcgcg ctgcggctga atacagaatc gcaccaggag
     1861 cgcattaccg cactggatag ccatctcaag caatttcgtt tcttttggga tggcatgccg
     1921 atgcaaccgc agattggcgt cagctactgc tatgtgcgct cgccagtgaa tcatatctac
     1981 ctgctgctgg gagagctaaa tacggtcgcc gaactttcca tcgtgaccaa cgccccggaa
     2041 aatatgcagc gtcgcggggc aatgtatttg caacgcgaat tgaaagataa agtcgcgatg
     2101 atgaatcgac tacagcaggc gctggaacac aaccattttt tcctgatggc ccagccgatt
     2161 accggtatgc gtggtgatgt ttaccatgaa attcttctgc gcatgaaagg tgagaatgat
     2221 gaactgatcg gccccgatag cttcttgccg gtcgcgcacg aatttggttt atcgtcgagt
     2281 atcgacatgt gggtcattga gcatacgctg caatttatgg ctgaaaacag agcgaagatg
     2341 cccgctcacc gttttgctat taatctgtct ccaacctcgg tatgtcaggc tcgttttcct
     2401 gttgaagtca gtcagttgct ggctaaatat cagattgaag cgtggcaact tatttttgaa
     2461 gtcaccgaaa gtaatgctct gaccaatgtt aagcaggcgc aaatcacctt gcagcatctt
     2521 caggaattag gctgccagat tgcgattgat gatttcggca ccggctacgc cagctatgca
     2581 cggcttaaaa atgtgaatgc cgatctgctt aaaattgacg gcagttttat ccgcaatatt
     2641 gtgtcaaata gtctggatta tcagatagtg gcatcgattt gccacctggc gcgaatgaag
     2701 aaaatgcggg tagtggcaga gtacgttgaa aacgaagaga tccgcgaggc ggtgctctct
     2761 ttggggatcg attatatgca gggttatctt attggtaagc cgcagccgtt aattgatacg
     2821 ttgaatgaaa tcgaacccat tcgcgaaagt gcctgaataa tgcgggccga catttctcgt
     2881 cggcccgcaa agcattaagc ggcgatttca ggtgtacttt cttcttcaat tttcaaccgc
     2941 cagccagcca cgccttccca gtattcttgc tccttttcca gatcaagcag taccagcgca
     3001 ttctgactaa accagtcatg cgggaaacgc agtgtccagt gactgtcatc agtaatcagt
     3061 gtcaatgttg gcggtgtggt ggttgcctga cgttgattgt ttagtaatac gccaaggcgc
     3121 aatagctgta tcagtggcag gaactgtttc ttcttaaaca aggtaaagcg cggcagatcg
     3181 tcgagcttaa tcgctttacg gtgatagcgc accagtgtcg ccatcatcag ctgctgttcc
     3241 tgattaaaac ccggcaagtc actgttttgc agaatataag cggagtggcg atgcaaaccg
     3301 ctgtggttga tattcaaccc gacctcatgc agcatggcgg cccatcgcag tagcgcctcc
     3361 agttgcggat gcgccagctt cggttgctgt tcccgccact gttcgtacat ttgcatagtg
     3421 gtatccagca cccgtcgggc ctgctcgctg tcgatgtgat actggttggc gaggctgctg
     3481 gcggtgcgac tacgcacatc ctgatgacgg aaacgtcctt ccatttcata cagtacgcct
     3541 tcgcgtaaag ccccgtcaga aaggcgcagt tcacggatgg ctaaagcatc aaacacaccg
     3601 cataaaatcg ccagtccagg aacgaagact gttttccgct cttcggaaag acccggtaaa
     3661 ctcagcgatg cgaaattacg gtgacgtaaa acttctttta ccagtttttc cagacgttcc
     3721 ggggtaatta tcccgtcttt ctcgcccatt tccattaaca cttcatgggc ggcttttatg
     3781 gtaccggaag cgcccattgc cacgttccag ccctgaatac ggaattgcca ggttaaagtt
     3841 tccagttttt gtgctgccgc catacgagcg cgctgaaaat tctctttatt gatgaccccg
     3901 ccagggaagt aaagctgggc aaagctgaca caacccatcc ggcggctttc aacgagaata
     3961 ggttcgaaat tttcaccaat caccagttcc gtagatccgc cgccaatatc aataaccagt
     4021 ttgcgacctt tctccggttg ggtatgttcc acgcccataa aaatcagacg ggcttcttca
     4081 ttaccggaaa taatttcaat cgggtaggga atgacctttt ccgcgcgttt cagaaagtca
     4141 gtggcgttca gcgcctgacg cagcgtatgg gtgccgacta tacagacgct ggcaggagaa
     4201 aacccttgta gccgttcggc aaacagcgac agacagttca aaccgcgcgt cattgcctct
     4261 tcactcaaca tattatctgg ccccaggccg tccgccagat gcacccgctg tttcaggcgg
     4321 ccaataatct gcatggcacc atctaccaca cgggctatga ccatgtgaaa actgtttgaa
     4381 ccaagatcga ccgcagcaaa ctcctgcgga cgaggggatt tatcgtgtat tggcataggg
     4441 ttattcaggt tgttcgagtg atttgatgta gtcgtaaatc gccaactgcg cccgtacttt
     4501 gcggcgattg ccgcggggaa cgtagcgatt actgagttct ttatcgatat aacgtgcttt
     4561 gaccgtatcg ctgaacaata tgtcgatgat gtccagcacc cgctgcttca ggcgcggatc
     4621 gagcagcggt gtcgccactt caatacgata atcaatattg cgcgtcatcc agtcggcgga
     4681 agaaaggtag acctttttat cgccgccatt ttcaaaaata taaacccggt catgttcaag
     4741 gtaacggtca acaatactga tggcacgaat gttgtcgcta atgccttcca gattggggat
     4801 cagcgaacac attccgcgaa ccagcagatt aaccggtacg ccggagctgg aggccgcata
     4861 cagacgatca accaggcctt tatcgacaag gttatttagc ttcagggtga taccactggg
     4921 cagcccttgc tgcgcgttgg cgatttcgcg gtctaccatt tcatataaca gacggcgaga
     4981 gttttgcggt gacaccatta aataatcaaa tgtcaccggg cggtatgggt tttcaataaa
     5041 gttaaatacc cgccgtactt cgttggtgat acgcgcatct gcggtcagca acgaatagtc
     5101 agtataaaga cgcgcggttt tttcgttaaa gttcccggtc ccgatgtgtg cgtaacggac
     5161 cacttcaccg ttttctttac gtgaaatcag gaacagtttg gcgtgaattt tcagccccgg
     5221 cgcagagaag ataacgtgca cgcctgcttc ggtcaggcgc ttcgcccagt gaatgttggc
     5281 ttcttcgtcg aaacgcgcct gtaactcaac caccacggtg actttcttac cgttatgtgc
     5341 ggcgtggatc atcgagtcga tgatgcgtga atctttcgcc acgcggtaaa tgttaatttt
     5401 aatcgccagt acgctcgggt cgaacgaagc ctgacgcagc agttccagca catgctcaaa
     5461 ggtgtgataa ggataataga gcaacacatc gcgttcgcga atggcatcaa aaccattgcg
     5521 gaactgggct ttatcaaacc aaatgtggcg taaacgcggc agtggtttgt tcaccagatt
     5581 ggctttgccg acattgggga aattaataaa gtctttaaaa ttatgataac gaccgccggg
     5641 gacgatggag tcgtagcggg aaatagtcag tttttcgcgt aacacttcaa ccagcgcatt
     5701 gggcatatcg cgctgataaa caaaacgcac cggctcagca gttaaacgct gcttgagact
     5761 ggaagacatc aactccatca ggctggcttc catctcatgc actaaatcgt attcggcatc
     5821 gcgggtcatc ttcattgaat aggcattcag cgcgtcataa tcaaagaagc ctttgaaaat
     5881 atcatcaagg cagtaacgca gaatgttatc cagaagaatc atcggcttgc gtcgacgcgg
     5941 tgcttctggc ggtaaattca caaagcgcgg cactttatct gatgggatct ccagcagcgc
     6001 gtaacggatg gtatcgccac ggataatttc caccgccaga taggtgtaat catctttcag
     6061 gaactgcact aagtcagtgt caggattgat taaaatcggc gtaatgtgct gacgcagata
     6121 ctgcttaaaa taatgacgaa gccagttttg ttgattgacg gagagctggc gttcattaat
     6181 caggaagatc tggttacgcg ccatctccag cagcagctcg ttgtagaggc cgtcgaattc
     6241 ctgatcggct ttcagcaccc gggactgaat tttgcccagt aaatggcggg aatgagagtt
     6301 ggagccttgt tcttcgctaa taatgatgcg tcgcttcagt tcagcgaagc ggactttata
     6361 gaactcatca aggttattgg aatagatccc caggaaacgc atcctttcaa tcagcgggtt
     6421 agatttgtcc gccgcttcct gaagcacgcg ttcattgaac gataaccaac tgagctcttt
     6481 ttcgatgtat agcttttcct gacccattac cacttttact ccgttttatt cacgggacac
     6541 tgcctggata ttatggcgag caatattggc agatgtccaa ctatgccaga aaagggggaa
     6601 aaaaactgct ggagcttgcg atttacaggt gaattgaaaa acgccagcgg cagagctggc
     6661 gctttaacta cggggcatta ctcgtcggca gcgtagccct gcggcggcag acgttgacca
     6721 tccagccacg cggcgttttc gtgcattttc agacgaccat cggcaaacca gctaatcacc
     6781 agtggataaa tagcgtgttc ctgggtttgc acgcgggcgg tgatgtcatc ttccgtatca
     6841 ccagcaaata ccgggacttt cgcctgtaaa ataaccgggc caccgtccag ttcatcggtg
     6901 acgaaatgca ccgatgtacc gtgctcttca tcaccatttt ccagcgcctg acgatgggtg
     6961 tgtaatccgg gatatttcgg cagcagagaa gggtgaatgt tcagcaaacg cccggcatag
     7021 tgggagacaa acgccgggct gagaatgcgc ataaaaccag ccagcacgac cacatcgggt
     7081 gcgtacatgt cgatttcatg aatcaactcc cggtcatagg cttcacgact gtcaaacgcg
     7141 ctggcgatga gcgtatgcgt tgcaataccc gcctggcggg cgcgttcaag gccgaacgcg
     7201 tcggccttat tgctgaaaac tgcccgtacg gtgcctttaa ttttgttagt tttacaggcg
     7261 tcaataattg cctgtaaatt acttccgttg ccggaaataa gcaccacaat attcattatt
     7321 cgataaccac gcgttgttcg gaatcagagg ctttgatgat accgattttc cacgcgtttt
     7381 caccgttggc gtttagcagg gcgagggctt tgtccacttc cggagcaggc agggcgataa
     7441 tcatcccgac gccgcagttg aaggtgcgat acatttcatg gcgctcaacg ttacctgccg
     7501 tttgcagcca gttgaacact tccggccact gccaggaaga ttcatcaatc actgcctgag
     7561 tattatctgg caatacgcgt ggaatgtttt cccagaagcc gccgccggtc aggtgcgcaa
     7621 tggcatgcac atcgaccttt tcaatcaact ccagcactga cttcacgtaa atgcgggtcg
     7681 gtgccagcag atgatcggct aatggcttac catcaagttc ggtggtttgc ggatcacaac
     7741 cgctgacttc aagaattttg cgcaccagcg aatagccgtt cgagtgcgga ccgctggaac
     7801 cgagtgcaat cagcacatcg ccgtcgctga ctttagagcc gtcgatgatt tctgattttt
     7861 ctaccacgcc aacgcagaaa cccgcgacat cgtaatcctc accgtgatac atccccggca
     7921 tttctgccgt ttcgccaccc accagtgaac agcccgattg cagacatcct tccgcgatac
     7981 cgctgatcac cgctgaagcg gtatcaacat ccagttttcc ggttgcgtaa tagtcgagga
     8041 aaaacagcgg ctctgcacct tgcaccacca ggtcattaac gcacatggcg accagatcaa
     8101 taccaatggt gtcgtgacgt tttaagtcca ttgccagacg cagcttggta cctacgccgt
     8161 cagtgccgga aaccagcacg ggttcacgat atttttgcgg caatgcacac agcgcaccga
     8221 agccgcccag accgcccatc acttccggac gacgcgtttt cttcactacg cctttgattc
     8281 ttccaaccag agcattaccc gcgtcaatat caacaccggc atctttgtag ctaagagagg
     8341 ttttatcggt cactgcttgg gtccccacgc gttacttgcg gtagaaaaat aaaattcggc
     8401 gcaattctaa cagggaaagc aaacgtttgc gagactgctt tacacaacct ttttgcacgt
     8461 cttttgccca ggcacgcggc gaaagaagac ttgtgccagg gtaaaggtta gttttcggat
     8521 ggaataatct tctttcataa ccatttgaat ataaaataac tttatctcaa atcgttatca
     8581 ttttgactaa agtcaacgaa aagaatattg ccgccttgaa gaaaggaggt ataatccgtc
     8641 gatttttttt gtggctgccc ctcaaaggag aaagagtatg aagatcgtgg aagtcaaaca
     8701 cccactcgtc aaacacaagc tgggactgat gcgtgagcaa gatatcagca ccaagcgctt
     8761 tcgcgaactc gcttccgaag tgggtagcct gctgacttac gaagcgaccg ccgacctcga
     8821 aacggaaaaa gtaactatcg aaggctggaa cggcccggta gaaatcgacc agatcaaagg
     8881 taagaaaatt accgttgtgc caattctgcg tgcgggtctt ggtatgatgg acggtgtgct
     8941 ggaaaacgtt ccgagcgcgc gcatcagcgt tgtcggtatg taccgtaatg aagaaacgct
     9001 ggagccggta ccgtacttcc agaaactggt ttctaacatc gatgagcgta tggcgctgat
     9061 cgttgaccca atgctggcaa ccggtggttc cgttatcgcg accatcgacc tgctgaaaaa
     9121 agcgggctgc agcagtatca aagttctggt gctggtcgct gcgccagaag gtatcgctgc
     9181 gctggaaaaa gcgcacccgg acgtcgaact gtataccgca tcgattgatc agggactgaa
     9241 cgagcacgga tacattattc cgggcctcgg cgatgccggt gacaaaatct ttggtacgaa
     9301 ataaagaata aaaataatta aagccgactt taagagtcgg cttttttttg agtaaagcgc
     9361 ctataacaca taatacagag gataatacta tgacgcgccg tgctatcggg gtgagtgaaa
     9421 gaccgccact tttacagaca atcccgctta gtttgcaaca tttgttcgcc atgtttggtg
     9481 caaccgtcct ggtgcccgtc ttatttcata ttaacccggc gactgtactg ttatttaacg
     9541 gtattggaac gctgctgtat ctcttcatct gtaaagggaa aattccggct tatcttggtt
     9601 ccagctttgc ctttatttca ccggtattgt tactgttgcc gttagggtat gaagtcgcgc
     9661 tgggcggctt tattatgtgc ggcgtgctgt tctgcctggt ttcttttatc gtgaagaaag
     9721 cggggaccgg ctggctggac gtgctgtttc cacctgcggc aatgggcgca atcgttgccg
     9781 tcatcggtct ggagctggcg ggcgtagctg ccggtatggc gggtttactc ccggctgaag
     9841 ggcaaacgcc agactccaaa accatcatca tctctattac caccctggcg gtcacggttt
     9901 taggttccgt gctgtttcgt ggtttcctgg caattatccc gattttaatt ggtgtgctgg
     9961 tggggtacgc gctctctttc gcaatgggaa ttgtcgatac cacgccgatt attaatgctc
    10021 actggtttgc gctgccaacc ctctatacgc cgcgcttcga gtggtttgcc attctgacta
    10081 ttctgccagc ggcgttagtg gttattgccg aacacgtagg gcacctggta gtaacggcta
    10141 atatcgtcaa aaaagatctg ctgcgcgatc caggtctgca ccgttcgatg tttgctaatg
    10201 gcttgtcgac cgtgatttcc ggcttctttg gctctacgcc aaataccact tacggagaaa
    10261 acattggcgt gatggcgatc acccgtgttt acagtacctg ggttatcggc ggggcggcga
    10321 ttttcgctat cctgctttcc tgcgtcggta aactggctgc cgctatccag atgatcccat
    10381 tgccggtgat gggcggcgtt tcgctgctgc tttatggtgt catcggtgct tccggtattc
    10441 gtgttttgat cgaatcgaaa gtggattaca acaaagcaca gaacctgatc ctgacttccg
    10501 tgattttgat catcggcgtc agtggcgcga aggtaaacat cggcgcggcg gagttaaaag
    10561 gtatggcgct ggcgaccatc gtcggtatcg gcttaagttt gattttcaaa ctgatcagtg
    10621 tgctgcgccc tgaagaagtg gtgctggacg cagaagacgc ggatataaca gacaaataat
    10681 gtaccgaccg ggcagtgttg ctgcccggtt caaacatcat gggattctgt ggtaaacttc
    10741 tcgcgatttt gtgaaatcct ggttgaggta tctctgaaca caccggcaca gctctctttg
    10801 ccactttatc ttcctgacga cgaaaccttt gcaagtttct ggccggggga taactcctct
    10861 ttactggctg cgctgcaaaa cgtgctgcgt caggaacata gcggttacat ctatctctgg
    10921 gcacgcgaag gcgcggggcg cagccatctg ctgcacgcgg cttgcgcgga attgtcgcag
    10981 cgtggcgatg cggtgggcta tgtcccgctg gataaacgca cctggtttgt tccggaagtg
    11041 ctcgacggta tggagcattt gtcgctggtc tgtatcgaca acattgagtg tattgcaggc
    11101 gatgagttgt gggagatggc gattttcgat ctctacaatc gaattctgga atcgggcaaa
    11161 acacgattgt tgatcaccgg cgatcgtcca ccgcggcagt tgaatctggg attaccggat
    11221 ctcgcgtcgc gactcgactg ggggcagatc tacaaattgc agccactttc tgatgaagat
    11281 aagttgcagg cgctacagtt acgcgcgcgt ttgcgtggtt ttgaactgcc ggaagatgtg
    11341 gggcgtttct tgctgaagcg gctcgacaga gaaatgcgca cgctatttat gacgttggat
    11401 cagttggatc gtgcgtcgat taccgcgcaa cgtaagctga ccattccgtt tgtgaaagaa
    11461 attctgaagt tgtagataag gtgtttattg tcggatgcgg cgcgaacgcc ttatccgaac
    11521 tacgcgaagt taatccatag tattcgtagg ccggataaga cgtggcaagc gtcgcatccg
    11581 gcaatgtgct tcaactagcg atggagcaca tcttaaccca cgatttccag tacctgctcc
    11641 ggtggacgac caatccgcgc tttgccattc gcgaccacaa tcgggcgctc cattagcttc
    11701 ggattatcca ccattgcctg aatcagcgcc tcttcgctaa gcgaactgtc agccaggttc
    11761 agttctttat aaagatcctc tttctggcgc atcaattcac gggcgctatt catccccagc
    11821 attttcagca aatcgcgcag cgttgccgca tcagcgggtg tctcaaggta gagcaccact
    11881 tctgactcca cgccgttttc tttcagcagg ttcagcgttt cccggctctt tgaacagcgt
    11941 gggttatggt agatttttac ctgtttggtc attgttcttc ctttaatgcg aattacatct
    12001 tggtataagg cttaaagcgt tcctgcagct ggcgcaactg gtcgatgcgc gcatcgtaac
    12061 gcgcttgttg caggctgcct aatttcacct gcgaactggc gctactcaac agcgaaatgg
    12121 cctgatcgag tcgtccggcg agcgcataac cttctgctcg cgcagccagc tcctgatcac
    12181 ggttatttag cgcggcttcc gcctgtgcca gcaaatccca gccgttgctg tcatctttat
    12241 tattaaaggt gtagcgattc agaatattgg ccgcttcttg tggttgaccg ccttgcagat
    12301 aagcgttcgc caggttgagc tgcaacaccg gatttgtgcg taaatcgcgg gcatttttca
    12361 ggcgattgat cgcttcattg gctttgtttt gcccaagatc gatatcagta gccagatcga
    12421 gataccatgc gttgccaggt tctgccgcca gtaacggttg cagcgtttta cgcgcctcgt
    12481 cgtatttatt ggcttccatc gcctgtaaag cacgaccata ttgcgccgct cgttgctgac
    12541 gaacgtttcc tttcgcccat tcatccagca aatcactggt gagctggtta cgtccggaat
    12601 tatacatccc cagtgtgcgc gctttcgcca gatagaaatc ttccgacgac tgcaccacca
    12661 tcggtcgcat ctgattagca cggttgcggg catctgccag acgactttcc ggcaacgggt
    12721 gagtcaataa aatttccggc gggcgcgagg agtaacgcgc ctgatcgagt aatttttcga
    12781 ggaaggttgg catcgcctgc ggatcgaatc ccgagcgttg cagcacctga ataccaatgc
    12841 ggtccgcttc ctgttcattt tgctgggtga aactgatcat cccctgacgc gttcccgcca
    12901 gtgtaccggt cagcgccgcc atccccgcct gcggactggc catcgccagt aaaatagaac
    12961 ctaacgcgcc gacccaggtc agcggcgcac tgcgttgttg atcttccatc gctcgcgcca
    13021 ggtgacgttg ggtgacgtgg gagatttcgt gcgccataac tgaagccagt tgactttcgt
    13081 tatcggaata acggaacagg gcagagtgca gcaccacgtt gccgccaaag aaagcaaagg
    13141 cgttaatttc gtcgttattg atcagaaaaa aatgaaacgg tgtcttaacc gaattggcat
    13201 gcgaaaccag acgcatcccc agcgaattaa tatattgcgt taacagcggg tcattaatta
    13261 acggcgcgct gccgcgtagc tggcggacat aatagtcgcc catctgcatt tcctgaccaa
    13321 tggaaagcgt gcttcctgcg gaggttccca tatccggcaa ggtgtctgcg ctgtcggcaa
    13381 acgccggggc tacctgacca atggtcatag cagcaatgag ggttgcaacc aggttttttt
    13441 tcaactgcct gaacataacc tctatcctgt atttctgaag atagtcattt gaccgatact
    13501 gttaaatatc gttcccaccg cgccgccttt gagtgtaacg gcgaagatta gcccactcaa
    13561 atgacttttc agtttcctga atccgtcata aaaagcgaag tcttttttgt gacatttcga
    13621 tacaattgcg gatcgcaatc ccgctcttga ggttcaggga aggtttttat gctcgaaatg
    13681 ttgatgcaat ggtatcgccg ccgttttagc gacccggaag cgattgcctt gctggttatt
    13741 ttagttgccg gatttggcat tatctttttc tttagtggcc tgcttgctcc gttgctggtg
    13801 gctattgtgc tggcctattt gctggaatgg ccaaccgtgc gcctgcaatc tattggctgc
    13861 tcccgccgct gggcgacgtc gattgtattg gtggtttttg tcggtatatt gctactgatg
    13921 gcgttcgtgg tactgcctat cgcctggcaa cagggcatct acttaatccg cgatatgccg
    13981 gggatgctca ataagctttc tgactttgcc gccacgttgc cgcgccgcta tccggcgtta
    14041 atggacgcgg gcattattga tgcaatggcc gaaaatatgc gcagtcggat gctgaccatg
    14101 ggcgattcgg tggtgaaaat ttccctcgcc tcgctggtcg gtttgctgac cattgccgtc
    14161 tatctggtgc tggtgccatt gatggtcttc ttcctgctga aagacaaaga gcagatgctg
    14221 aacgccgttc gtcgggtgct gccgcgtaac cgtggactgg caggacaggt gtggaaggag
    14281 atgaatcaac aaatcaccaa ctatatccgc ggcaaagtgc tggagatgat cgtggtgggg
    14341 atcgccacct ggctggggtt cttgctcttt gggctgaact attcgctgct gctggcggtg
    14401 ctggtcggct tctcggttct tattccgtac attggcgcat ttgtggtgac cattccggtg
    14461 gttggcgtgg cgctattcca gtttggcgca ggcacggaat tctggagctg cttcgcggtg
    14521 tatctgatta ttcaggcgct ggacggcaac ctgctagtac cggtgttgtt ctccgaagcg
    14581 gttaacctgc atccgctggt gattatttta tcggtggtga tcttcggtgg tttgtgggga
    14641 ttctggggcg ttttcttcgc cattccattg gctacgctga tcaaagccgt gatccacgcc
    14701 tggcccgatg ggcaaatcgc gcaagaataa tcttcagccc gacgaactgt ttcgccgggc
    14761 tgatttttca ggattcctga cgtaaataaa tagcccgata acaaatactc accagcactg
    14821 caccgccgat aatattaccc agcatcaccg ggagcagatt ggcggtaata aaatggctga
    14881 ccgtcagtgc cggaaaattg tctgcgctac tgtgcgccag ttgccagaag gaggtagggg
    14941 cgaaatggcg aatggcaatg gcgaacggaa tcacaaacaa attggcgata tagtgctcaa
    15001 agccactggc gacaaacagg gtgatgggca aaatcatggc gacgattttg tcgcataacg
    15061 aacgcccgca ataactcatc cacagcgcca ggcaaaccat cagattgcac ataatgccga
    15121 ggctgacaga ttcagtaaat gtatgatgca ttttgccctc ggcgcagtgt aaaaccgcga
    15181 ctccccacat cgcgttttca ctcatcacca gcccggaaaa ccagattaac aaactgaaac
    15241 aggcaatacc tgccagatta ccgcaggcca ccagaagtgc gttaatcagc caagttcgcc
    15301 aactaataac gccccgactt tttgccatta ccgtcattac cgacgaggtg aacaggttgg
    15361 tgccgcaaac cgccagcaaa ataaagccaa gtgtaaagca aaggccgccc accagatggg
    15421 ttaatgcctg tgaagacggg gcatcggcga taactgaaag gtaaaaagta aatccgatcg
    15481 ccataaacac gccagcggac atcgccatta aaaagaccga cactttactg cgggcaattt
    15541 tatgggcagc aatccgttca gcgattgccg cttttctggc gctcaactgc aagtcgaaag
    15601 agagtttgtt tctcatgcat actcctgcaa aagcagatta caacacctcg cgaaccgaga
    15661 ttcccagacg ctgcattcgt gacagcagcg tggtgcgctt catccctaat cgtgtcgccg
    15721 cgccacgggg cccggcaaca atgccattgg tttctcgcaa tacctgaaca atgcgctgac
    15781 gttcttcttc gtcattctct ggcgtcgtcg ggtgcagcaa ctgcgccatt gaactgcgaa
    15841 gcgctgaatc ttcatttagc gtcggcagta aacggctttg tcggacatta agatgtaaat
    15901 tcagactgtt accgcgagtc agcagtaccg ctcgctcaat cacgttttcc agctcgcgca
    15961 cgttgcccgg ccagtcccac gacatcaact ggcgtagcgc ctcggtcggg atggcgtcaa
    16021 ttgaacgatt catatggcgc gccatttttt gcgtgaaatg ctttgctaaa agagggatat
    16081 cttccggacg gtcgcgcagc ggcggcaatt ccagtgggaa gacattcagg cgataaaaga
    16141 gatcgctgcg aaactggcga tcttcaacca tttgccacaa atcacggttg gtggcggcaa
    16201 tgactctgac atttaccggg atcgttctac tcccgccgag acgctcaatc tcccgttcct
    16261 gcaatacgcg cagcagttta ggctgaagtt ctaacggcag atcgccaatt tcatcgagaa
    16321 acaacgtgcc gccatcggca atttcaaaac gaccacgatg ggtattaatc gcaccagtaa
    16381 acgccccttt gtcatgaccg aataactcac tttccagaag actggcgggg atggcagcgc
    16441 agttgatttt gaccagcggc ttgtcgcgtc gcgggctaag ttgatggatc gctctggcga
    16501 tcacctcttt gccggttccg gtttcaccgc aaatcaacac tgtactgtcg ctcttcgcca
    16561 caatatctac ctgctggagt aggtcttcca ttgcctggct ttgatagata atgtcaccga
    16621 tgcccagatt cgaaaggagc tgctcgctaa gctggtggtt ttcttgctgc aaactttcct
    16681 gcaaatcggt catgctacgc caggcatcgg cattgccaac ggcaatagcg atgcgatcgg
    16741 ctatgtgttg tagtagctgg cagttttcct cactaaagag agtggaagag gtatgcgcca
    16801 gcaacaatgc acccggtgta tggttgccaa aggtaagcgg tataaggagc gcagattcgc
    16861 agccgttaga tgcaagtaag agtaataacg ggtcgcgttg ccagagaaac agatcgtctg
    16921 cacggtgcgt caaggtcggt tgtcgggttt gtagcgtttg tgtcagcaat acactgtcgc
    16981 caggcatatt gcgtggcaga cacgcacaat gtgaggcaga aagatcgctg caccacaggc
    17041 tgaacttctc gttctttcga tgatcgccca gtaccatact gactgaagcc agaccgaaaa
    17101 aatgatggat ctcacgagcg acgtcagcga tcagatcgtc gagatcaaga tgtgacagca
    17161 ctgcattagt gatatccacc agtacccgat gatgatcgcg atctttctcc agcaaacgca
    17221 gcgccacctg ctgttcttca tcattgagcc atgattgcag aaccggggtg ataatgcgtg
    17281 ccagccagtt aaacagtgtg caatcctggg gagtaaaacg gcagccgtgg gtgcgaataa
    17341 agcgtagctc cgccattcca ctactgccga gaaggacggc gtgacgcgaa taatcgatca
    17401 tctcgccctg atactctgct gttgccgtca catcgctgcc atcatcatgg tagcggtaat
    17461 agacgttgct tttgagaaca tgcagttcaa taagttgtac cgggctgaac ggcggcggca
    17521 gcgggatgaa ggcgcgtaat aaagacgcct taccgtgttt ctgcgctaac cgctccgcga
    17581 gcattccgtt taccgcttca attgttattc cttgcggcgg ggcaaacatc gcctcgtctg
    17641 acatagccat caacagtcct tattaatgag aaatccttct cacgcaattt ttctggccat
    17701 cagatagatg gccggtgcct gcacaatttc atccaacaga tgaatgaatt gaatgctacg
    17761 cgcctgccac acagacgttg cgtcatctgc cagtggcgcg aatgcccggg tcaacagggc
    17821 ggtgtggctg gcgtcaataa caatctcacc aaaagtcatc acccccagca tcttacgtcg
    17881 ggcttgctcc tcttcgacct gggcaagcca atcttcgtat tccgtcagtg ggcagcggaa
    17941 ggcgacattc agacaatcaa tcacgccaac gtgatggccg atagccagcg agtaatacat
    18001 tacctgagag ctgtgttccg gcatctcgtc gctactggcg acaaactttt ttcgcagcgt
    18061 ccagaaaaca atttccccgc actcttcagt catgtaaccc ccggagtacg cgaaagagtt
    18121 gctgaacgat actattgaga cgtggatcgt cggcatcgcg caaccaggtg ttaacccgat
    18181 ttcctgtagg atcgtcgctt aaatggcgca ggagccgatc gcaaatttct cgcccctgac
    18241 gatagcccgc cagccgccgc gcttctcgct caatggcgat acgctgtgat ggcgggatct
    18301 gcggccacaa cggttgcata gtcaccccgg tgggatcgcg ataatccacc gcgtgaatct
    18361 tctgttgcag caaaccgagc gccacggcga aaccgtgaat ggtggcggcc ggtgttggcg
    18421 ggcagccggg gatccaaaca tcaatgggga caatggtgtc gctgccgccc cagacgctgt
    18481 agagatcgtg gaaaatccgc cgccggcacc gcacgcgccg taggaaacac agattttatg
    18541 atcgggggca gactcatacg cccgaagtgc aggcatacgc atcgcccggg tgactgcgcc
    18601 agtaaataac aaaatatcgg cgtgacgcgg tgatgaaaca accttaatgc caaaacgttc
    18661 tgcgtcgaat actggtgtaa tggcggcaaa aatttcgatt tcacaggcgt tgcagccacc
    18721 gcaatcgacg cgataaacgt aggctgagcg acggatatcc tgtagcaaat gccgcttcat
    18781 cttttgcgtt tgctcgtcca gcgtgatggg ctggctgaca tgttgtgtaa gcactggact
    18841 catagctgct ccttagccac cagcggtaca tctgtatcgt cgttgagcag cgtcgcgcgt
    18901 tgtttgcact ccgggcagac gctcgcttgc gcccataaca tttcgcggtt ttgtggcgca
    18961 ttttgttgct gtgccaacaa ttcagcagcc agtgcgacgg ttttttgctg ggcaaacggg
    19021 cgttcgcaac ggctgcaacg ttgtagatgg aacgtcgcgc gggtatagag atcggcttta
    19081 ttggtgacgg tcagttcaaa gttattggta agctggatgg ctctggtcgg gcacacttct
    19141 tcacaacgtc cgcagtaaat acaacgcccc agatagagct gccaggtgcg cgaattttgc
    19201 tggtcgtcgg tctggatagt cagcgcattt gccggacaag cacaggcgca ggcaccgcag
    19261 gcaatacatt ggctgggcat caggtccggt ttcccgcgaa agcctgggct gacctccagt
    19321 ggcgcgaagg gatatttcac cgtcgcggtt ccggcgcgca taatagtttt cagtaacttc
    19381 agcatcttct gtccttactt cagcggcgaa cggttacgat caatgccgta gcgttcgatc
    19441 tctttatacg gcacggtttt tgactggcgc ttgcgcacat ctaccagcgt cacacggtcg
    19501 gtacaggagt agcagggatc aaggctaccg ataatcagcg gtgcgtcaga aacggtattg
    19561 ccgcgcagca tgtaacgcaa caccggccag ttggcgtagg tggcggcacg gcagcgccag
    19621 cggaacaatt tttggttatc accgagcatg ctccagtgca cgtcttcacc gcgtggcgct
    19681 tcaacaaagc ccagcgcgaa tgcgtgaggt ttatagctaa agccttcggt cagcagtggg
    19741 gtatccggca tgttgtcgag ggcaaattcc agcattgcca gcgaatcaaa cgtctctttg
    19801 acacggacca tcacgcggga gaaaacatcg ccgccggtaa aggtaaacag tgtttttgga
    19861 atattgccgt agtcggcgta ggggtgatca aagcgcaaat cacgggcgat ttgtcggtcg
    19921 agaatgccaa tgccctgagt gcgttgttcc atattcggcg tagcaagcag catctctacc
    19981 agctccgaca cgtcggcgcg catctcgcgc accagtttca gcgtttgcag acgttgctct
    20041 ttgagaatat cgcggcgaac accaccaatc agattcagac cgtaggtttt acgcgacccg
    20101 atcagcaatt ccgccatcgt catcgacttt tcccgcacgc ggaaaaattg cataaagccg
    20161 gtatcaaaac caacgaaatg gcaggagagg ccaaggttaa gcaaatgact gtgtagccgt
    20221 tcgacttcca gcagaatcga gcgaatagta tgtgctcgtt gcggcacctc aatccccagt
    20281 gcattttcaa ccgaattggt ataggccaca ctgtgggcaa aaccgcaaat cccacacacg
    20341 cggtccgata agaaggtcac ttcgttgtag cccatccgcg tttctgccag tttctccatg
    20401 ccgcgatgga cataaaacag gcggtaatca gcatcgacaa tttgctcgcc atccacaaac
    20461 aagcggaagt gacccggttc atcggaggtg atatgcagcg ggccgacagg gatcacccgc
    20521 gcatcgctgt tgccctcatt gataaacgca tacgtttcgg aatctgtcgt cggttcaggg
    20581 cgcagtcgat aatccatcgc atctttgcgc agcggatgca tatcttccgg ccagtcatcg
    20641 ggcaacacca ggcgacgctg atccggcagg ccaaccggaa tcagcccgta catatcgcga
    20701 atttctcgct cgccccagac cgcggcaggg acgcgcggtg tgactgacgg aaactcccga
    20761 ctgtcggcat cgaccagtgc cttcaccacg atccagcatt tttcggcccc ttccattgaa
    20821 agggcataat aaaccgcgta atgaccgtta agtgtccgct cgtcgttgcc aaacaggact
    20881 ggaagccagc catcatgttg ataataaaga taatgtacaa cgtcaggcag cagattgatt
    20941 ttcaccgtaa tggtgacctg ttcaggcgtt tgtcgctctt catccagcac cgcgccgggg
    21001 aactgcgttt tcagggcggc gagaatcgct tcgccacaat ttgacgatga attaacgttc
    21061 actgtgtttc tccgaaacag atgcggtgcc tgaaggcaaa aaatcatgcc aggtgctacg
    21121 ttgtgcaggc aggtcgtgcg tccctgagag gactatagtg gaagcgcccg ccaggatcct
    21181 gatgacaggt tgtggaatat gcgttcccat cgccagcatc atgaccagca gaatcaccat
    21241 tggcgaggtg gtcaaccagc cgagatcacc ccggttaacg gcctgcggcg gtttcgccat
    21301 taacacccgc gcagccatcc gtaccaggcc cgccagcacc agcgttaaca gcaataacag
    21361 caggacgata atcagcaggt gattacgtgc cagtccggcg gtaacggtca taaattcgct
    21421 aagaaaaatg ttgaagggcg gcatccctgc cagcgccagc gccagcgcac cgccgccaaa
    21481 cagcacggcg gtaaatggca tgattttgag catcccacag acgacgttga gatcgcgcgt
    21541 gccgtacttg agcagtacat tgccggaacc gcagaacagc agcgtttttg ccagactgtg
    21601 gtttaaggtg tgcagcagcg cggcaaaaat tcccagtggc ccgccaatgc ctagcgccac
    21661 cgcgaccagt cccatgttct ccacgctgga gtacgccagc agacgcttaa tgtcccgctg
    21721 taccagaatg aaaaatgccg ccacggcaac cgacaacatg ccgaagatga gcaacaaccg
    21781 gttggggaaa tcgctgccga tggcctggca gataatgatg taatagcgaa ttagcaccaa
    21841 cagcgcgcag ttcagcaata cggcggagag cagggcgctg accggactcg gtgcttcact
    21901 gtgagcatcc ggcagccagg cgtgcatggg aaatagcccg gttttggtgc caaagccaat
    21961 tagcacaaac acaaaggcca acagcattaa tgttgggtca agcaaggacg attgcttaag
    22021 aacctcgctc cagaatatcg ccatttctgc ctgcggcata acgctggcga cgttggcgta
    22081 taccagcacg gtaccgaaca gaccaaaagc gacaccaaca gtacaaataa tgatgtactt
    22141 ccatgctgct tccagcgatg aacgctgacc gtaaatgcct accagaaacg ccgagcttaa
    22201 ggtggtggct tcgatcgccg cccacatcac aatcaggtta ttgctggtaa caaccagcag
    22261 catggtaaac aaaaacagat ggaagaaacc gtagtaatcg cacagcgtaa cgggggaaag
    22321 ctcgccgtgt gccacttcat gacgcatgta gccaatcgag taaacaccgg tgagaaagcc
    22381 aatcacacca agaatggcga ggaacaaacc gcccagacca tcaatatgaa gccacagtcc
    22441 cgcagcgaat atttctcctg catcagcggc agtttggacc acccagagtg ccagaatcag
    22501 cagcagtgtg atccctaagc tatgtaatac agtcaccgta caagtcgcag agagcccccg
    22561 tttccggcag gcaaaacaga gcagcgaaaa aagcagcggc gtgagcagga gtaaagcgaa
    22621 catcacagaa taactcatct cattatccct tcagcgcggt caagttgttc acgtccagcg
    22681 tgccgtgggt acgccatatt tttcttgcca gtaacaccat cacaatgacg gcgaagatgg
    22741 cgtcggtagc gatacctatt tccaccagtt ccggtgctcg ccaggcaaga agcgccagca
    22801 ccagatggga gccgttttcc atcaggcagt aaccaaaaat ttgccgcagg atattgcgct
    22861 ggctgacaat gcacagcagg ccaagcagaa aatgacctaa cgctaccgcc agcgccggtt
    22921 tcagcccggt agccatcggt agcttcacgg gctgaacgac aaatgcgcaa agcaggacaa
    22981 ttaacgccgc gagcagtgcc atcattgccg gaccgaataa cgctttttcc gggatgttct
    23041 ggggaatatt tcgtgcagcg taagtcatga ttaacggtac cagcagcact ttggtgataa
    23101 aggcactggc ggaccagatc agcagttgct ctgcggcgaa caggcacgag agagtggcga
    23161 aaatagacac cagcaccagc gactggcagg cgtaaaatcc gcatgacagg cgatagcttt
    23221 tgacgctaat cacaaacagc gatgtcagca tcatcagtcc cgccagatta tttacgatca
    23281 tagaaccggt cattcatcat ctccttactg caaccaggtc gcggcgatta cgctggaaat
    23341 cagcgacatc acaatcagta caatcaacac caggcgcatt gaccctggca gcggtgcggc
    23401 atcggcgacg gcctcgctcg gtttgccagg cacaacgcga ccaaaccagc gaataaacca
    23461 ggcgaaactg gcgaccgatt caatcatcag cagaatcatg gcgggcagca ggatccagta
    23521 ctccactgac aacgcaaaac aggcggcaaa cagcgggaat ttactgaaga agccgttgaa
    23581 cggcggcaca ccggtaatcg ccagcgctgc cacgcagaaa cccacgcctg gcaacggcag
    23641 ggtgtgcaat acgccacgca gacgcggcaa caagcgcgtg ccgcagctgt aactcagcgc
    23701 acctgctaca aggaaaaaca ggcttttagc gaacgcgtgg ttgacgatgt aggcgatgct
    23761 gccctccagt gccagccgcc cactttcacc atcgatgcgg cgtggagata ggcgctgatc
    23821 ggtgttggcg cttccattgc gtccggtagc cacgcttgca tcggtagctg ggccgatttc
    23881 ccccacgcgg caaacaggat gccgccataa accagataac gtgcgtcgcc atgtaactcg
    23941 ctcatcgcgc taagcacaaa cgttccggtc tgcaaaaaca gcgtggcggc ggcaagatac
    24001 aaccccaacg aaccgatatg ggtgataagc aacgctttta gtgctgaacg ctgcgcttta
    24061 tcgctctggt aataactgat caacgcccag gagcagccgc cctaatttca aaaaacaaca
    24121 actgaccgag cagcgtcgag gagagtacca gtcccgccat cgcgccgata aacaccagca
    24181 aaaatgcgta ataacgattc gtgccgttat gcgggtgttc gcgattttta tccgtcagat
    24241 aacccgtgga gtagatcgtg accagcaaac cgagaaacac caccacaaac agaatcagcg
    24301 tactcacgcg atcaatgacc aggccaaaca atgccacatc ccccacgttg accaacgtca
    24361 acgtgacggc aactttatcg gcctgataaa acgcggagat cagcgacaac atgcacagcg
    24421 tggtcagtgc ggcgaataaa acccccattc ggcggcccga cgttgtggcg aaaacgaaac
    24481 gaccagtgcg ccgataaaag gcagcattaa cgtcgtcaga gcaagatttt ccatattccg
    24541 tcagtgctcc ttacagaccg gttaaccaga agacccatgc aagcgcagca aggctgaagc
    24601 caagccaggt cacatggtga atgagtaaaa aacgcccgcg tgccagcgtg ttttctgcga
    24661 ttgaggccag tacaaaaatc agcaaaacct tgagcagcgt aacgacaagt gaagtcagca
    24721 ggcaggcgag agaaagttct tgcgcgcgcc caaagagcag aaacagggcc acaaacagtg
    24781 ctgccatcac gacctgtttc agccccagcc cccatttcgc tagcgccagc ccggcaccgg
    24841 aatattcggt cagcgggcct tcctgtaatt cctgttctgc ttcagcaaca tcaaagggaa
    24901 tttttcccat ctcaatgaag caggcaaaac cacaggccag taacgccagt acggtggtta
    24961 gcggcgagtt ccagcccgtt gccagcgtat tgctgatcat ctcgatatgc gtggaacctg
    25021 ctatcagcgc caataccagc agtgagagaa tgagcatggg ttcgaccaga atgccgagcg
    25081 tcaactcgcg actggcaccg actcccgcaa acgggcttcc ggtatccagc ccggaaagag
    25141 caaagaaaaa acgaaacagg gcaagaagat agataagggt gatcagatcg ccgccgcccg
    25201 caaaagggga aacggtaata aaaagtggta gggccatcgc cagcaccagc atgctgctga
    25261 ttaataccca cggcatcagg cggaacatca gacctgaaga tataggcgca acttcctggc
    25321 gtttaaacag tttgtggata tcgcgataat cctgccagat ccccggcccg cggcgggagt
    25381 gcatacgcgc gcgtatctgc cgggaaatac ccgtaaaaag cggggttagc agcagaatca
    25441 cggcctgtgc taacgcaaaa atgaccagat atccgtcgca aagagtttgt ctcatggtga
    25501 tttccttaga cggcaatagc gattagcagc acaaccagtg cggcgaccac atacaggcaa
    25561 tagagacgaa agtcgccgct ttgcagatgc tgtatttctt tggccagccg ttgggtggcg
    25621 ctcacgatgg ggcggatcac ccgctcatcc cagaagggtt ctgtgctctg agccctggcg
    25681 gtgacgtgcg caagaccttt atttagcctc agcgtagggt cgaactgttg tcgtagacga
    25741 aatagcgcag aaaagaccac acgcagcggc tgcatcacgc cattgcctga cggggccatc
    25801 gcattttccc agccgtagcc gcatgcccag gcatctcctg tgcgacgaaa cgctgcgcgg
    25861 cgcgaacgac accacagcca gtaaaggcca ggcagcaaag gcatcgccag tagtaacagc
    25921 aacagtaatg agggggtgac ccgtgtatga aacgtgccgg gtacaagtgc tattccgctg
    25981 gcgacagtga cgggaggggt attggtaaac gcatgggcaa tatgcattat cttcggtgcc
    26041 agccaactgg cactaagcgc aattaatacg cagagtgccg cgagcagtag catggcgacg
    26101 atcatcgtat ttggcacttc ctgtgcctct tcagcgtgtg tactgcgcgg cgcaccacag
    26161 aaagtaatac cgtacatttt gacgaagcac attactgcca gcccaccagt gactgccagc
    26221 attaccatag caataggacc cgcaagttgt agcgctacgg cttccacacg acttagtgag
    26281 aacagcgact gccaggtgta ccattcgctg ataaaaccat tcagcggagg aatggctgat
    26341 atcgcgaggc aaccaatcag gcatgctgcg gctgtccacg gcatccgttt cgccagtgcc
    26401 cccatttttt ccatgtcgtg ggtatgcaaa cgcgaaataa tcgcacccgc gccgagaaat
    26461 agcagccctt tgaacagcgc atggttgagc agatgaaaca gtgcgccgag cagtccaacc
    26521 acggtgagca gcgggtcgtg cagtgacaga ccgtccatcg ccacaccgac cgccagcaaa
    26581 ataatgccga cgttttcgac ggtactccag gccagcagcc gtttgatatc ctgttccgcc
    26641 agtgcataca gcacgcccag gagcgcggag attgcgccga tcgccatcac cagagtgccc
    26701 caccacagag gcaaacccgt ttgcgccagc agatccatcg ctactttcag gatgccgaaa
    26761 ataccgattt tgaccattac gccagacatc aacgccgaag cgtgcgatgg tgcggcaggg
    26821 tgagcgcgcg gcaaccagct gtgcaacggc atcatcccgg ctttcgcgcc aaaaccgaaa
    26881 aaggccagca gaaacaccgc cgacgccagc cccggagaaa gtgaaagcgt gcgaaactgg
    26941 caaaatcgag gctgccgctt tcgcgcccca tcagcaagaa ggcgatcata atcagcaccg
    27001 atccggcgtg ggcgataaaa aagtagagca tgccagcgtt gatcgacgtt ttatcctgcc
    27061 tggcaatgac cagaaaccag gaagacagcg acatcatttc aaacagcacg atgaaccaaa
    27121 aagcgttgtc catcaccagc agggcaacca tcgatgcgat gaaaagattc ataaagaagc
    27181 ccatcgccgc cgcgcctttg ccctcgtatt cgcgcatata agtcaatgaa tagagcgaac
    27241 aaaccaccac cagcaaggag ataaccagca ccataaatgc cgagaggcta tccattcgca
    27301 gggttaaatc ggcaaagtca aaaggggtgg caaaatgggc gactaaagtt acaccagcat
    27361 gtaattgcgt aattccgctg atgatgccaa tcacgccgcc caccagcgat gtgatgccgg
    27421 aaagcttaat agccagtctg tccagaccga gtaaaaacag cgaagccaga ctggcaaaca
    27481 gatagaggat cagcgaccag gttaataatt gcagggcatc cattagtgtt gctcctgagt
    27541 gagggaaagg ggatcctccc cgccattggc aaaccagctt gcggcaaggc gctgtttttc
    27601 tttcatctga cgttgcaggc tatcgtcggt gatcagccgt aacgcctgat tcgggcaagc
    27661 gcgaacgcag gccggacctt ctggcaagaa atcacacagg tcgcatttca ccgcgacggt
    27721 ctggacaccc ggttcccagc gcagcaacgc atgttggggc agcacgtttt cttcgccgtc
    27781 tttgagtgag ccttcagcct gaaaaacata ttgcgcatgg gcattcaccg gacggcttcc
    27841 tgaagcgctg attgcgccaa atgggcagac cacggcgcaa agcttgcagc caatacagag
    27901 gctttcgttg agttggatcg catcatccct ctgagagatg gcattgaccg ggcagacctg
    27961 caggcaaggg gcttcctcac agtgatgaca cacgacaggg gcagtgattg ttgacgtctt
    28021 cgccagggcc aggcgcgggt gttgctgtaa accttgcgtt ttatgcacgt ccgaacaggc
    28081 agcgagacag gtattacatc ctgtacacca cagtggttcg gccaccacaa agcggttcat
    28141 gtcggctcct tgaaaatgat atgaaacatt gcatttcata ttgcataaag gatgccattc
    28201 tgattaactc aatatattca ataagttatg tattgactgg attctgaacg gtacaaaaat
    28261 gatggtcatt aacgttcagc aaaaaatgga ttcgacaccc gtgtcgcagg ggaaagcgag
    28321 aatgtgatct tgatcacgag aagtgaggta tgctggccgc aagcgcagcc agcacggaat
    28381 ggagcaaagt aatcaggcgt gttctttcag ccagttcagc acaacgtcgt ggtgattgct
    28441 ggttttgaaa tcgtcaaaga catgttcgat tttgccatca gcgtcaatca ggaagctgat
    28501 gcgatgaatg ccatcgtagg ttttgcccat gaaggacttt tcaccccaga cgccgaattg
    28561 ttcgcacacc tggtggtcct catcagacag gagcgtaaag ttaagcagct ctttttccgc
    28621 aaaacgggag agtttttcgg gtttatcggt gctgataccc agcacatcaa cgcccgcttt
    28681 tttcaactca tccatgttat cgcgtaagcc gcaggcctgt acggtacagc cgggggtcat
    28741 ggctttcggg tagaaataaa ccagaacacg ctgtccctgg aagtcggtca aattaacttg
    28801 ttctccgtct tgatccggca agctaaattt cggtgcgata tcaccggctt tcagtggatt
    28861 cattacttaa ctccatcctg ttcatcatgt tgggaataat tgacgacgtt aatactgcct
    28921 tgtgcattga gttctgtaca tagggctttg aacgcttgct caatatttgc tgcgtccgca
    28981 gatgcggggc tgtgggcggt tatctgaata tgcaactgcg cagcccgttc attttcagca
    29041 ggttgcgtgc gcgacaccag ctccgcaatg ttcatatgat gcgcgtcgaa aagtgctgtg
    29101 aagcgttcaa ttaaatgcgg ggagtctgcc acatcgacct gaacccagac agatgctggc
    29161 attggcggac gcggacgcgc cgtcgtgcgc ttcatcacga ttaaaagatc cagttcggca
    29221 cctttcaacg gtaacgttga ttcaatcaga gtaatggcat tccatgaacc ggaaagcagc
    29281 ataataaacg tgaactcttc tcccagcatc gccaggcgac tgtcttcaat attacagccg
    29341 caactactga catgacgggt gatggtgttc acaattccag ggcgatcggc acccaacgca
    29401 gtgatcacca gataatgttg cgatgacagt gtcaaactgg ttattccttt aaggggtgag
    29461 ttgttcttaa ggaaagcata aaaaaaacat gcatacaaca atcagaacgg ttctgtctgc
    29521 ttgcttttaa tgccatacca aacgtaccat tgagacactt gtttgcacag aggatggccc
    29581 atgttcacgg gaagtattgt cgcgattgtt actccgatgg atgaaaaagg taatgtctgt
    29641 cgggctagct tgaaaaaact gattgattat catgtcgcca gcggtacttc ggcgatcgtt
    29701 tctgttggca ccactggcga gtccgctacc ttaaatcatg acgaacatgc tgatgtggtg
    29761 atgatgacgc tggagctggc tgatgggcgc attccggtaa ttgccgggac cggcgctaac
    29821 gctactgcgg aagccattag cctgacgcag cgcttcaatg acagtggtat cgtcggctgc
    29881 ctgacggtaa ccccttacta caatcgtccg tcgcaagaag gtttgtatca gcatttcaaa
    29941 gccatcgctg agcatactga cctgccgcaa attctgtata atgtgccgtc ccgtactggc
    30001 tgcgatctgc tcccggaaac ggtgggccgt ctggcgaaag taaaaaatat tatcggaatc
    30061 aaagaggcaa cagggaactt aacgcgtgta aaccagatca aagagctggt ttcagatgat
    30121 tttgttctgc tgagcggcga tgatgcgagc gcgctggact tcatgcaatt gggcggtcat
    30181 ggggttattt ccgttacggc taacgtcgca gcgcgtgata tggcccagat gtgcaaactg
    30241 gcagcagaag ggcattttgc cgaggcacgc gttattaatc agcgtctgat gccattacac
    30301 aacaaactat ttgtcgaacc caatccaatc ccggtgaaat gggcatgtaa ggaactgggt
    30361 cttgtggcga ccgatacgct gcgcctgcca atgacaccaa tcaccgacag tggtcgtgag
    30421 acggtcagag cggcgcttaa gcatgccggt ttgctgtaaa gtttagggag atttgatggc
    30481 ttactctgtt caaaagtcgc gcctggcaaa ggttgcgggt gtttcgcttg ttttattact
    30541 cgctgcctgt agttctgact cacgctataa gcgtcaggtc agtggtgatg aagcctacct
    30601 ggaagcggca ccgcttgcgg agcttcatgc cccggctgga atgattttgc cggtgacctc
    30661 cggtgattat gcaatcccgg tgaccaacgg tagtggtgct gtcggtaagg cgctggacat
    30721 tcgtccacca gcccagccgc tggcactggt ttctggcgcg cgtacccagt tcacgggcga
    30781 taccgcttca ttgctggtgg aaaatggtcg tggcaatact ctgtggccgc aggtggttag
    30841 cgtgctgcag gcgaaaaact acaccatcac ccaacgtgat gatgctggtc agacactgac
    30901 caccgattgg gtacaatgga accgtctgga cgaagacgag cagtatcgtg gtcgttatca
    30961 aatctctgtt aagccgcagg gttatcagca ggcggttacg gttaaactgc tgaacctgga
    31021 acaggcgggc aaaccggttg cagacgcggc ttccatgcag cgttacagca cggagatgat
    31081 gaacgttatt tccgccggtc tggataaatc tgccactgac gccgcgaacg ctgcgcaaaa
    31141 tcgtgcctcc accactatgg acgtacaaag tgcagctgat gacaccggtt taccgatgct
    31201 ggtcgtacgc gggccgttca atgtggtttg gcaacgtctg ccagcggcgc tggaaaaagt
    31261 gggcatgaaa gtgaccgaca gcacccgttc gcagggtaac atggccgtaa cttataagcc
    31321 gctgtctgac agcgactggc aggaactggg cgcgagcgat ccaggcctgg catccggtga
    31381 ctataaactg caggttggcg atttagataa ccgcagcagc ctacagttca tcgatccgaa
    31441 aggtcatact ctgactcaga gtcagaacga cgcgctggta gctgtcttcc aggctgcgtt
    31501 tagcaagtaa aaatacaggg ctggaatcat ccggcccttt tttctgatat gatacgcaaa
    31561 cgtgtgcgtc ggcaggaaaa cgcgatttta gcggtaattc gcacgaaatt tgtttgtcgg
    31621 acgtagttcg gataaggcgt tcacgccgca tccgacaaaa catccggcac accagacagc
    31681 aaaagatttt aaaacgttaa ttcacaccca ggagtgataa agatgcaaaa gcaagctgag
    31741 ttgtatcgtg gtaaagcgaa aaccgtatac agcacggaaa acccggacct attggtgctc
    31801 gaattccgca atgatacgtc agcaggggat ggcgcgcgca ttgagcagtt tgatcgcaaa
    31861 ggtatggtga acaacaagtt caattacttc attatgagca aactggctga agcgggtatc
    31921 ccgactcaaa tggagcgtct gctctccgat accgaatgtc tggtgaaaaa gctggatatg
    31981 gtgccggttg agtgtgtcgt gcgtaaccgt gctgctggct ctctggtgaa acgtcttgga
    32041 atcgaagaag gtattgagct gaacccgccg ctgttcgatc tgttcctgaa aaacgacgcc
    32101 atgcacgatc cgatggtcaa cgaatcttac tgcgaaacct ttggctgggt gagcaaagag
    32161 aacctggcgc gtatgaaaga gctgacctac aaagcgaacg acgtgctgaa aaaactgttc
    32221 gatgatgctg gtctgattct ggtcgacttc aagctggagt ttggcctgta caaaggtgaa
    32281 gtggtactgg gcgatgagtt ctccccggac ggcagccgcc tgtgggacaa agaaacactg
    32341 gagaaaatgg acaaagaccg tttccgccag agcctcggtg gcctgatcga agcctatgaa
    32401 gccgtcgccc gccgcctggg tgtacagctg gactgatttt tctggttgct tgtttcttta
    32461 gccggagggt actccggcta aatgtctttg atacccccgc tacaatactt ctcattatct
    32521 ccctttgaca ggaaagtcat accaggccaa tatgtatata cataacgtat atcccaaaaa
    32581 gggggggcta tggaaactac tgtattttta agtaatcgca gtcaggccgt cagattaccg
    32641 aaagcggtcg ctctgccaga agacgttaag aaagtagaaa ttattgcgat tggtcgtaca
    32701 agaatcatca ctcccgccgg tgagtcctgg gacagttggt ttgatggcga aaatgtgagt
    32761 gcggatttta tggatatccg tgatcaaccc gcaatgcagg aaagggaatc attttgatgc
    32821 tgaaattcat gctggatacc aatatctgta ttttcactat taagaataaa ccagtacatg
    32881 tcagagagcg gtttaacctg aattctggcc ggatgtgtat aagttctgta acattgatgg
    32941 agcttatcta tggggcagaa aaaagtcaga tgccagaacg caatttggcg gttattgaag
    33001 gtttcgtatc tcgtttggta gtgcttgatt atgatagtgc tgctgcgacg cataccgggc
    33061 aaatcagggc cgagcttgcc cggcagggaa ggcctgttgg accttttgac cagatgatag
    33121 cgggacatgc tcgcagtcgc ggtttgattg tggttaccaa caatacccga gagtttgagc
    33181 gagtggcggg aatcagacta gaagactgga gctaacattc tcttttactt ctaacattcg
    33241 taaattatat aacgtggcat gaagccttaa ttcctccgcc atttcatccc gctttcctgt
    33301 ggtaatcttc ctctgaacca cctacgatca tttctataat gaatatattg ttgaggtatc
    33361 tatgcgttgg caagggcgac gtgaaagtga caatgttgaa gacaggcgca acagctccgg
    33421 tggtccgtct atgggcggtc ctggttttcg cctgccaagc ggtaaaggcg ggctgatttt
    33481 actgttagtc gtgctggttg caggctacta tggtgttgat ttaaccgggt taatgactgg
    33541 acagccggtt tcccaacaac aatcaacgcg gtccattagc cctaatgaag acgaagctgc
    33601 aaaattcacc tcggtgattc tggcaaccac ggaagacacc tggggacaac agttcgagaa
    33661 gatgggtaag acctatcagc aaccgaagct ggtcatgtac cgtggaatga cgcgcaccgg
    33721 ctgcggggct ggtcagtcca ttatggggcc gttctattgc ccggcggatg gcacagttta
    33781 tatcgatctc tccttctatg atgacatgaa agacaaactt ggcgcggatg gcgattttgc
    33841 ccaggggtac gttatcgccc atgaagtcgg tcatcatgtg cagaaactgt taggcatcga
    33901 gccgaaagtt cgtcaactgc aacaaaacgc gacgcaggcg gaagtgaacc gcttatctgt
    33961 gcgtatggaa ctccaggccg actgttttgc cggtgtctgg gggcataaca tgcagcagca
    34021 aggcgttctg gaaaccggcg atctggaaga ggcgctgaac gcggcgcagg ccatcggcga
    34081 tgaccgttta caacagcaaa gtcaggggcg agtagtacca gatagtttca cacatggcac
    34141 ttctcagcaa cgctacagct ggtttaaacg tggtttcgac agcggcgatc cggcacaatg
    34201 caatactttt ggtaaaagca tttaacttcc ggggcaggga tggctgaact gactgcgctt
    34261 cacacattaa cagcgcaaat gaaacgtgaa gggatccgcc gcttgctggt gttgagcggg
    34321 gaagagggtt ggtgttttga tcatgcgctt aagttgcgtg atgccttacc tggcgactgg
    34381 ctgtggattt cgccgcagcc agatgctgaa aaccactgtt caccctcggc gctacaaact
    34441 ttacttgggc gcgagttccg gcatgcggta ttcgacgccc gccacggctt tgatgccgct
    34501 gcctttgcag cacttagcgg aacgttgaaa gcgggaagct ggctggtttt gttactccct
    34561 gtatgggaag agtgggaaaa ccaacctgat gccgactcgc tgcgctggag tgattgccct
    34621 gaccctattg cgacgccgca ttttgtccag catctcaaac gcgtacttac ggcggataac
    34681 gacgctatcc tctggcggca aaaccagccg ttctcgttgg cgcattttac tccccgtact
    34741 gactggcacc ccgcgaccgg cgcaccacaa ccagaacaac agcaactctt acagcagtta
    34801 ctgaccatgc cgccgggcgt ggcagcggta acggctgcgc gtgggcgcgg taaatcggcg
    34861 ttggcagggc aactcatttc tcgtattgcg ggcagtgcga ttgtcaccgc gcccgcaaaa
    34921 gcggcaacgg atgtattggc acaatttgcg ggcgagaagt ttcgctttat tgcaccggat
    34981 gccttgttag ccagcgatga gcaagccgac tggctggtgg tcgacgaagc cgcagccata
    35041 cctgcgccgt tgttgcatca actggtatcg cgttttcctc gaacgttgtt aaccactacg
    35101 gtgcagggct acgaaggcac cggacgtggt tttttgctga aattttgcgc tcgctttccg
    35161 catttacacc gttttgaact gcaacagccg atccgctggg cacagggatg cccgctggaa
    35221 aaaatggtta gtgaggcact ggtttttgac gatgaaaact tcacccatac accacaaggc
    35281 aatatcgtca tttccgcatt tgaacaaacg ttatggcgaa gcgagccaga aacgccgtta
    35341 aaggtttatc agttattgtc tggtgcgcac taccggactt cgccgctgga tttacgccgg
    35401 atgatggatg caccagggca atatttttta caggcggctg gcgaaaacga gattgccgga
    35461 gcgctgtggc tggtggatga ggggggatta tctcaacaac tcagtcaggc ggtatgggca
    35521 ggttttcgtc gcccgcgggg taatctggtg gcccagtcgc tggcggcgca cggtagcaat
    35581 ccactggcag cgacattgcg tggacggcgg gtcagccgga tagcagttca tccggcgcgt
    35641 cagcgggaag gcacagggca gcaacttatt gctggtgctt tgcaatatac gcgtgacctc
    35701 gactatcttt cggtgagttt tggttacacc ggggagttat ggcgtttctg gcaccgctgc
    35761 ggttttgtgc tggtgcggat gggtaatcat cgtgaagcca gcagcggttg ctatacggcg
    35821 atggcgctgt taccgatgag taatgcgggt aaacaactgg ctgaacgtga gcattaccgt
    35881 ttacgtcgcg atgcgcaagc tctcgcaaag tggaatggcg aaacgcttcc tgttgatcca
    35941 ctaaacgatg ccgtcctttc tgacgacgac tggcttgaac tggccggttt tgctttcgct
    36001 catcgtccgc tattaacatc gttaggttgc ttattgcgtc tgctacaaac cagtgaactg
    36061 gcattaccgg cgctgcgtgg gcgtttacag aaaaacgtca gcgacgcgca gttatgtacc
    36121 acacttaaac tttcaggccg caagatgtta ctggtccgtc agcgggaaga ggccgcgcag
    36181 gcgctgttcg cacttaatga tgttcgcact gagcgtctgc gcgatcgcat aacgcaatgg
    36241 caattttttc actgactcct tcaattttct cccatggtca ttgtgagtca gcggcgtaaa
    36301 attaatgaca tcagttaagg agtttgccat gaaacatgac cattttgttg ttcaaagccc
    36361 ggataaacct gcgcaacagt tgttactgct tttccacggt gtcggggata acccggtggc
    36421 aatgggggaa ataggtagct ggtttgcacc gctgtttcct gatgcgctgg tggtgagtgt
    36481 cggtggcgcg gagccgagcg gtaatccggc ggggcgtcag tggttttcgg tgcagggtat
    36541 tacggaagat aatcgccagg cgcgtgtgga tgcgatcatg ccgacgttta ttgaaacggt
    36601 gcgctactgg cagaaacaga gcggagtagg ggctaacgcc acggcgctca tcggtttctc
    36661 tcaaggcgcg attatggcgc tggagagcat taaagccgaa ccgggccttg cttcacgcgt
    36721 cattgctttt aacggacggt atgcgagcct gccggagact gcgtctacag ccacgacaat
    36781 tcacctgatt catggcggtg aagatccggt tatcgatctg gcgcatgcgg ttgcggcgca
    36841 ggaagcgtta atcagtgccg gtggtgacgt gacgctggat atcgtggagg atttaggcca
    36901 tgcgatcgac aatcgcagta tgcaatttgc cctggatcac ttgcgttata ccattccgaa
    36961 acattatttc gacgaagcgc taagtggcgg taagcctggc gatgatgatg tgattgaaat
    37021 gatgtaaaag acagattacc tggcctgata agacgcgtga gcgtcgcatc aggcatcgtt
    37081 caccattgcc ggatgcggcg ttaacgcctt atccggccta cggttgagca ctcagcttac
    37141 ttcttcggtt gatccttttt cggccagtcg tcgtcatcgt cccacttatc gttgaaatca
    37201 cgatgcgggg gaagctccgg cttgttggcg agaaatttct tatggtcgac gcgcttgaga
    37261 tctttaatca cgttcagcag gacgcctacc aaaaagacaa tcaccagaat ccaccaatat
    37321 ttagccagcc agtccatgct tatttcctct tgcagaacca ctcatcaggc gacgagctgt
    37381 tccatgatac gttgatacat acgggcaagt agctgcaggt cggcagcgtt cacacattca
    37441 ttaattttat gaatagtggc attgaccggc ccgagttcca ccacctgcgc ccccatgcga
    37501 gcaataaagc gcccgtcgga cgttccgcct gtggtcagta gctgcggttt aatttcatta
    37561 tagtgctcaa ccgcgttaac gaccgcatcc accagtttac cgcgcgcggt caaaaatggc
    37621 tgcccggaaa gccaccagtc caccgtataa cgcagttgat gtttttcaag cagggcaagc
    37681 acctgtgctt tgatcatctc atcagtcagt tcggtgctga agcggaagtt aaactgcaca
    37741 aacagttcac ccggaataac attgttactg cccgttcccg cctgaatatt agcaatctgc
    37801 atactggtcg ccgggaaaaa ttcattgccc tgatcccact caatagccac taattcatta
    37861 aggaaaggtg ctgcgcgatg tactggattg tcagccagat gcgggtaggc cacatgcccc
    37921 tgaacgccat gaatggtaag gttgcaggtt aacgatccgc gacgaccatt tttcaccaca
    37981 tcaccaacca cttcgatact cgacggttcg ccgaccaggc agtaatcgag acgctcatta
    38041 cgtgccatca acgcttcgac gacttttacc gtaccgttgt gggcactggc ttcttcatca
    38101 gaggtgatca gaaatgccag tcgccccgta tggttgggat gttgtgcgac aaagcgttct
    38161 gccgctacca ccatcgccgc cagcgagcct ttcatatctg ccgcaccgcg tccgaataac
    38221 atgccatcac gaatggtggg ttcaaacggc ggattgatcc aacgatcggc gtcgccaggc
    38281 ggcaccacgt cggtatgccc ggcaaaggcc aacgtttcac cttgcccacg ccatgcccaa
    38341 aaattctgcg tatcggcaaa atccatgcgt tcaacggtaa aaccgatcgc ctgcaaacgt
    38401 tcaatcaaca aagcctggca tcctgcgtca tcaggactca gggaagggcg gcgaataagc
    38461 tgttgtgtca gctcaataac cgggcacgac atagactaca cctcatggaa aaattgctga
    38521 taactggaat cactgaaacc cagcagcata ggcttaccag gcgcgcagag caatggacgt
    38581 ttgataattg caggcatttc agtcattaat gccgccgcag aggccgcatc ggtgatttta
    38641 ttgcgggtgg tttcgtccag tttacgccag gttgtaccac gggtgttcag taacgcttcc
    38701 cagcctaatt cgttgataaa accgttcaat aattcgctgt ccagcccatc gacgcggtaa
    38761 tcatgaaaac gatagtcgat gttattggct tccagccaac ggcgagcctt tttaatggtg
    38821 tcacaatttt tgatgccgta aagtgtaacc ataatgaatc ctttttcgat ctatgagaat
    38881 aaatactcat ttaagggtga aaacattttg cctgc
//
LOCUS       DAJOWB010000033        37346 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJOWB010000033 DAJOWB010000000
VERSION     DAJOWB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.033"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            124..3876
                     /gene="yfaL"
                     /locus_tag="OHO85_001877"
     CDS             124..3876
                     /gene="yfaL"
                     /locus_tag="OHO85_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCQ2671253.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAVTDSCQGYDVKASCQ
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIVNNTAFTNNTAEGYGGAIYTNSVTAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSSAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQN
                     QAELNVGSTQQTFVHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITSDVDTRSDAAGHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILHYASSLWVGDGATFVTGADQDIQSIDAISSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSNVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYLNNLRAANQAF
                     MMERRDHAGGDGQTLNLRVIGGDYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGTDGEW
                     MLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWFQHGNQKQGAWLDSWLQYA
                     WFSNDVSEQEDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQLDDFTAA
                     NRARVSQSQGDDIQTRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAV
                     KQRGEIKVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(4004..4726)
                     /locus_tag="OHO85_001878"
     CDS             complement(4004..4726)
                     /locus_tag="OHO85_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCQ2671254.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            4873..7500
                     /gene="gyrA"
                     /locus_tag="OHO85_001879"
     CDS             4873..7500
                     /gene="gyrA"
                     /locus_tag="OHO85_001879"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCQ2671255.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7649..9337
                     /locus_tag="OHO85_001880"
     CDS             7649..9337
                     /locus_tag="OHO85_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HCQ2671256.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERAAEKTPVRQRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGIAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9334..9957
                     /locus_tag="OHO85_001881"
     CDS             9334..9957
                     /locus_tag="OHO85_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCQ2671257.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANEALKVHDSKWLKSNGIASQYLPPEMTLKPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWILNDSNPNFIGIYRLNFLAR"
     gene            9981..14495
                     /locus_tag="OHO85_001882"
     CDS             9981..14495
                     /locus_tag="OHO85_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCQ2671258.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPFEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGKELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     IGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVTLNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVRVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQAIGVTQQPEYWQHMLDSYAEQADNVPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDPSDINDSLIFDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYARDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14496..16145
                     /locus_tag="OHO85_001883"
     CDS             14496..16145
                     /locus_tag="OHO85_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCQ2671259.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYARLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMAAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKKPQWRRILQAETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16150..16926
                     /locus_tag="OHO85_001884"
     CDS             16150..16926
                     /locus_tag="OHO85_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCQ2671260.1"
                     /translation="MRKIFLPLLLVALSPVVHSEGVQEVEIDAPLSGWHPVEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(17000..18184)
                     /gene="atoB"
                     /locus_tag="OHO85_001885"
     CDS             complement(17000..18184)
                     /gene="atoB"
                     /locus_tag="OHO85_001885"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HCQ2671261.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(18215..19537)
                     /locus_tag="OHO85_001886"
     CDS             complement(18215..19537)
                     /locus_tag="OHO85_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HCQ2671262.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAVKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(19534..20184)
                     /gene="atoA"
                     /locus_tag="OHO85_001887"
     CDS             complement(19534..20184)
                     /gene="atoA"
                     /locus_tag="OHO85_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HCQ2671263.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(20184..20846)
                     /gene="atoD"
                     /locus_tag="OHO85_001888"
     CDS             complement(20184..20846)
                     /gene="atoD"
                     /locus_tag="OHO85_001888"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HCQ2671264.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(21042..22427)
                     /gene="atoC"
                     /locus_tag="OHO85_001889"
     CDS             complement(21042..22427)
                     /gene="atoC"
                     /locus_tag="OHO85_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HCQ2671265.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(22424..24250)
                     /gene="atoS"
                     /locus_tag="OHO85_001890"
     CDS             complement(22424..24250)
                     /gene="atoS"
                     /locus_tag="OHO85_001890"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HCQ2671266.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            24417..27266
                     /gene="rcsC"
                     /locus_tag="OHO85_001891"
     CDS             24417..27266
                     /gene="rcsC"
                     /locus_tag="OHO85_001891"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCQ2671267.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(27466..28116)
                     /gene="rcsB"
                     /locus_tag="OHO85_001892"
     CDS             complement(27466..28116)
                     /gene="rcsB"
                     /locus_tag="OHO85_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCQ2671268.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(28133..30811)
                     /gene="rcsD"
                     /locus_tag="OHO85_001893"
     CDS             complement(28133..30811)
                     /gene="rcsD"
                     /locus_tag="OHO85_001893"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCQ2671269.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEQDATATGNNDNDNEKEGTDSVSIHF
                     NSTKIEIASALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRH
                     FSSRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLP
                     HLNLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKL
                     KQAQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLV
                     DEIQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRR
                     GDRDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDN
                     LHFPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAD
                     PEVEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTD
                     NPSNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESP
                     LGGDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLV
                     PGKQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            31550..32653
                     /gene="ompC"
                     /locus_tag="OHO85_001894"
     CDS             31550..32653
                     /gene="ompC"
                     /locus_tag="OHO85_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCQ2671270.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            32765..33820
                     /gene="apbE"
                     /locus_tag="OHO85_001895"
     CDS             32765..33820
                     /gene="apbE"
                     /locus_tag="OHO85_001895"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCQ2671271.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            33894..34958
                     /gene="ada"
                     /locus_tag="OHO85_001896"
     CDS             33894..34958
                     /gene="ada"
                     /locus_tag="OHO85_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCQ2671272.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPEKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            34958..35608
                     /gene="alkB"
                     /locus_tag="OHO85_001897"
     CDS             34958..35608
                     /gene="alkB"
                     /locus_tag="OHO85_001897"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCQ2671273.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            35684..37327
                     /gene="yojI"
                     /locus_tag="OHO85_001898"
     CDS             35684..37327
                     /gene="yojI"
                     /locus_tag="OHO85_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCQ2671274.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         9127 a         9203 c        10022 g         8994 t
ORIGIN      
        1 tattcatcaa ataataacaa aatagccatt gcaccgggtt gaacaattta caaaagaaag
       61 atattccata tcgtataatg cgattaaata cgccgtctta tagaaaatga tggttagatt
      121 aatatgcgga ttatctttct acgcaaggag tatttatctt tactcccgtc aatgattgca
      181 tctcttttct ctgctaacgg tgtcgcggcg gtcactgatt catgccaggg atatgatgtc
      241 aaagcgagtt gtcaggccag caggcaaagc ctttcaggca ttacgcagga ctggagtatc
      301 gctgatgggc aatggctggt tttttcggat atgaccaata acgccagcgg tggggccgta
      361 tttttgcaac aaggagcgga attttcacta ttaccagaaa atgaaactgg aatgactctg
      421 tttgccaata acaccgttac aggagaatat aataacggcg gggccatatt tgctaaagaa
      481 aactcaacgc tgaatcttac tgatgttatt ttttccggta acgtcgcagg cggctatggt
      541 ggcgcaatct attcttctgg tactaacgat actggtgccg tcgatttacg tgtcactaac
      601 gccatgtttc gcaataacat cgctaatgat ggcaaaggtg gcgcaattta taccattaat
      661 aatgacgttt atttaagtga tgttattttt gataacaacc aggcatatac atcaacaagt
      721 tacagtgatg gcgatggcgg ggcaatcgat gttaccgata ataatagcga cagcaagcat
      781 ccttcaggtt atacgatagt aaataacact gcctttacaa ataacactgc cgaaggttat
      841 ggcggggcga tatataccaa tagcgtgacg gctccctatc ttattgatat ttctgttgat
      901 gacagctaca gccagaacgg aggcgtgtta gtcgatgaga acaatagcgc agcaggctat
      961 ggagatggtc cttcctctgc ggcgggtggc tttatgtatc tcggcttaag tgaagttacc
     1021 tttgatattg ccgacggaaa aacgctggtt attggcaata cagagaatga cggagctgtt
     1081 gactctattg ctggtaccgg gttaatcacc aaaacaggtt ccggcgatct ggtacttaat
     1141 gcagataaca atgactttac tggtgagatg cagattgaaa acggtgaagt taccctgggc
     1201 cgcagcaact ccctgatgaa tgtcggcgat acgcattgcc aggacgatcc gcaagactgc
     1261 tacggtctga cgatagggag tattgatcag tatcagaatc aggctgagct aaacgttggc
     1321 tcgacccaac aaacttttgt gcacgcattg acgggctttc agaatggcac tttaaatatc
     1381 gatgctggtg gcaacgttac tgttaatcag ggcagttttg ctggcatcat cgaaggtgct
     1441 ggtcagctca ccattgcgca aaacggcagc tacgtgctgg caggggcgca gtcgatggcg
     1501 ctaaccggcg atatagtcgt tgatgatggt gcggtgcttt cgctggaagg cgacgcggca
     1561 gatcttaccg ctctccagga cgatccgcag tcgatcgtgt taaacggcgg tgtgctcgat
     1621 ctctctgatt tctccacctg gcagagcggc acatcataca acgatggcct tgaagtcagt
     1681 ggcagcagcg gaacggttat cggcagtcag gatgtggtag atcttgcagg tggcgacaat
     1741 ttgcatatcg gcggcgacgg gaaagatggc gtctacgtgg tggtcgatgc gagcgacggg
     1801 caggtaagtc tggcaaacaa taatagttat ttgggcacaa cacaaatcgc ctccggtacg
     1861 ctgatggtga gcgacaactc gcagcttgga gatacccact ataaccgcca ggttatcttt
     1921 accgataagc aacaagaaag cgtgatggag attacctccg acgttgacac gcgttcagat
     1981 gcggcaggcc acggacgtga tattgaaatg cgcgccgacg gtgaagtggc agttgatgcg
     2041 ggggtagaca cgcagtgggg cgcactgatg gctgacagca gcgggcagca tcaggatgag
     2101 ggtagcacat tgactaaaac gggggctggt acactggagc tgaccgccag cggtacaacg
     2161 cagtcggcgg tacgtgtcga agaaggcacc ctgaaaggtg atgttgctga tatccttcat
     2221 tatgcttcgt cactgtgggt tggtgatggg gcaacgttcg ttactggcgc ggatcaggat
     2281 attcagtcaa ttgatgctat ttccagcggc actatcgaca tcagcgatgg tacggttttg
     2341 cgcctgaccg ggcaggatac ttccgtcgcc cttaatgcct cactatttaa cggcgatggg
     2401 acgctggtga atgccaccga tggtgtgacg ttgacaggtg agcttaatac caaccttgaa
     2461 actgacagcc tgacttatct ttccaacgtg acggttaatg gcaatctgac caatacgtcc
     2521 ggtgcggtta gcctgcaaaa tggcgtcgct ggcgatacgc tgacggtaaa cggtgattat
     2581 accggcggcg gtacgctact gctcgatagc gaattaaacg gcgatgactc ggtaagcgat
     2641 caattggtga tgaacggtaa tactgctggc aacacaactg tggtggttaa ctccattaca
     2701 gggattggtg agccgacatc gacaggcatt aaagtggttg atttcgcagc tgatcccacg
     2761 cagtttcaaa acaatgcgca gttcagtctg gcaggcagcg gctacgtcaa tatgggagcg
     2821 tatgactaca cgctggtgga agataacaac gactggtatc tgcgatcgca agaagtaacg
     2881 ccgccatcgc cacctgatcc agacccgact cccgatcctg atcccacgcc ggatcctgat
     2941 ccaacacccg acccggaacc tacgcctgct taccagccgg tgttgaatgc caaagttggc
     3001 ggttatctca ataacctgcg ggcggcaaat caggcgttta tgatggagcg acgcgatcac
     3061 gcaggtggcg atggtcagac gctgaattta cgtgttatcg gcggagatta tcattacaca
     3121 gcagcggggc aactggctca acatgaagac acttctacgg tgcaacttag cggcgatctg
     3181 tttagcgggc gctggggcac ggatggcgag tggatgcttg ggattgttgg tggctacagc
     3241 gataaccagg gcgacagccg ctcgaatatg accggaactc gcgccgataa ccagaaccac
     3301 ggttatgccg ttgggctgac atcaagctgg tttcagcacg gtaatcagaa gcaaggggcc
     3361 tggctggata gctggctgca atacgcgtgg tttagcaatg atgtttccga acaagaagat
     3421 ggcacagatc attaccactc gtcggggatt atcgcctcgc tggaggcggg gtatcagtgg
     3481 ttaccggggc gtggtgtggt gattgaaccg caggcgcagg tgatttatca gggcgtgcag
     3541 ctggatgatt ttaccgccgc taaccgtgcg cgcgtgtcac aatcgcaggg tgatgatatt
     3601 cagacgcggc tgggtttaca cagcgaatgg cgtaccgctg ttcatgtcat accaacatta
     3661 gatctgaatt attatcacga tccccattcg acggaaattg aagaggatgg cagcactatc
     3721 agtgacgatg cggtgaagca acggggtgaa ataaaagtgg gagtcacggg caatatcagt
     3781 cagcgagtgt ccctgcgcgg cagcgtggcg tggcagaaag ggagtgatga ttttgcccag
     3841 acggcagggt ttttgtcgat gacggtgaaa tggtaatcac tgtctagtcg gcacggttgc
     3901 cggatgcggc gtaaacgcct tatccggcct acggggagca tttgtaggcc tgataagacg
     3961 cggcaagcgt cgcatcaggc attggcgcgg ccaaacatca acctcactta ttctgcgtgt
     4021 gcagcatata gttcacatcc acgccggggc cgagtttaaa agtattagtg atcgggttgt
     4081 aatgcagccc agtgatatgc cgctctttta aactggtctg atccacccag cccagcaatt
     4141 ctgccggttt aataaacttc ttcacatcat gcgtgccttt gggcaccatg cgcaaaatat
     4201 attccgcacc aaccaccgcc atcagccatg acttgccgtt gcggttaagt gtcgagaaaa
     4261 agacatcgcc gcctggtttc accagttgcg cacaggctct gaccactgac tgcggatcgg
     4321 ggacgtgctc cagcatctcc atgcaggtca ccacatcata ctgcccggca tgttttgccg
     4381 cgtgctcttc cacggtttcc tgcacataat ccacctgaat gccgctttcc agcgcgtgca
     4441 gttttgccac ctgcaatggc tcaaagccca tatccagacc ggtcaccgtc gcgccttcgc
     4501 gcgccatact ctcggccaga atgccgccgc cacaaccgac atcgagcacc tttttgccaa
     4561 ataaaccgcc agcacgctcg gcaatatagc ccagacgcag cgggttaatg cggtgcaacg
     4621 gtttgaactc accttcaaga tcccaccagc gggaggcgac ggcttcaaat ttagcgatct
     4681 cttcgtggtc tacgttatgg ttttccggcg atttttcggc attcattggc acttctactc
     4741 cgtaattggc aagacaaacg agtatatcag gcattggatg tgaataaagc gtataggttt
     4801 acctcaaact gcgcggctgt gttataattt gcgacctttg aatccgggat acagtagagg
     4861 gatagcggtt agatgagcga ccttgcgaga gaaattacac cggtcaacat tgaggaagag
     4921 ctgaagagct cctatctgga ttatgcgatg tcggtcattg ttggccgtgc gctgccagat
     4981 gtccgagatg gcctgaagcc ggtacaccgt cgcgtacttt acgccatgaa cgtactaggc
     5041 aatgactgga acaaagccta taaaaaatct gcccgtgtcg ttggtgacgt aatcggtaaa
     5101 taccatcccc atggtgactc ggcggtttat gacacgatcg tccgtatggc gcagccattc
     5161 tcgctgcgtt acatgctggt agacggtcag ggtaacttcg gttccatcga cggcgactct
     5221 gcggcggcaa tgcgttatac ggaaatccgt ctggcgaaaa ttgcccatga actgatggcc
     5281 gatctcgaaa aagagacggt cgatttcgtt gataactatg acggcacgga aaaaattccc
     5341 gacgtcatgc caaccaaaat tcctaacctg ctggtgaacg gttcttccgg tatcgccgta
     5401 ggtatggcaa ccaacatccc gccgcacaac ctgacggaag tcatcaacgg ttgtctggcg
     5461 tatatcgatg atgaagacat cagcattgaa gggctgatgg aacacatccc ggggccggac
     5521 ttcccgacgg cggcaatcat taacggtcgt cgcggtattg aagaagctta ccgtaccggt
     5581 cgcggcaagg tgtatatccg cgctcgcgca gaagtggaag ttgacgccaa aaccggtcgt
     5641 gaaaccatta tcgtccacga aattccgtat caggtaaaca aagcgcgcct gatcgagaag
     5701 attgcggaac tggtaaaaga aaaacgcgtg gaaggcatca gcgcgctgcg tgacgagtct
     5761 gacaaagacg gtatgcgcat cgtgattgaa gtgaaacgcg atgcggtcgg tgaagttgtg
     5821 ctcaacaacc tctactccca gacccagttg caggtttctt tcggtatcaa catggtggca
     5881 ttgcaccatg gtcagccgaa gatcatgaac ctgaaagaca tcatcgcggc gtttgttcgt
     5941 caccgccgtg aagtggtgac ccgtcgtact attttcgaac tgcgtaaagc tcgcgatcgt
     6001 gctcatatcc ttgaagcatt agccgtggcg ctggcgaaca tcgacccgat catcgaactg
     6061 atccgtcatg cgccgacgcc tgcagaagcg aaaactgcgc tggttgctaa tccgtggcag
     6121 ctgggcaacg ttgccgcgat gctcgaacgt gctggcgacg atgctgcgcg tccggaatgg
     6181 ctggagccag agttcggcgt gcgtgatggt ctgtactacc tgaccgaaca gcaagctcag
     6241 gcgattctgg atctgcgttt gcagaaactg accggccttg agcacgaaaa actgctcgac
     6301 gaatacaaag agctgctgga tcagatcgcg gaactgttgc gtattcttgg tagcgccgat
     6361 cgtttgatgg aagtgatccg tgaagagctg gagctggttc gtgaacagtt cggtgacaaa
     6421 cgtcgtactg aaatcaccgc caacagcgca gacatcaacc tggaagatct gatcactcag
     6481 gaagatgtgg tagtgacgct ctctcaccag ggctacgtta agtatcagcc gctttctgaa
     6541 tacgaagcgc agcgtcgtgg cgggaaaggt aaatctgccg cacgtattaa agaagaagac
     6601 tttatcgacc gactgctggt ggcgaacact cacgaccata ttctgtgctt ctccagccgt
     6661 ggtcgcgtct attcgatgaa agtttatcag ttgccggaag ccactcgtgg cgcgcgcggt
     6721 cgtccgatcg tcaacctgct gccgctggag caggacgaac gtatcactgc gatcctgcca
     6781 gtgaccgagt ttgaagaagg cgtgaaagtc ttcatggcga ccgctaacgg taccgtgaag
     6841 aaaactgtcc tcaccgagtt caaccgtctg cgtaccgccg gtaaagtggc gatcaaactg
     6901 gttgacggcg atgagctgat cggcgttgac ctgaccagcg gcgaagacga agtaatgctg
     6961 ttctccgctg aaggtaaagt ggtgcgcttt aaagagtctt ctgtccgtgc gatgggctgc
     7021 aacaccaccg gtgttcgcgg tattcgctta ggtgaaggcg ataaagtcgt ctctctgatc
     7081 gtgcctcgtg gcgatggcgc aatcctcacc gcaacgcaaa acggttacgg taaacgtacc
     7141 gcagtggcgg aatacccaac caagtcgcgt gcgacgaaag gggttatctc catcaaggtt
     7201 accgaacgta acggtttagt tgttggcgcg gtacaggtag atgactgcga ccagatcatg
     7261 atgatcaccg atgccggtac gctggtacgt actcgcgttt cggaaatcag catcgtgggc
     7321 cgtaacaccc agggcgtgat cctcatccgt actgcggaag atgaaaacgt agtgggtctg
     7381 caacgtgttg ctgaaccggt tgacgaggaa gatctggata ccatcgacgg cagtgccgcg
     7441 gaaggggacg atgaaatcgc tccggaagtg gacgttgacg acgagccaga agaagaataa
     7501 ttttacttct tcatgccaaa agggagctat ctcccttgtt tgaattgaaa agtccaggct
     7561 gcaaagtctg ggcttttgtc gtattagggc acggtaaagt ttggctgtgc ccgtaaaaaa
     7621 tggctggcta tacacaagga atgtggcaat gagtggtgaa aaaaaggcga aaggctggcg
     7681 gttctatggt cttgtaggtt ttggcgcaat agcactgctt tccgctggcg tctgggcgtt
     7741 gcaatatgct ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct
     7801 gcaaatcgat ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc
     7861 caaagatctc ctcaccattc cgtttctcca cgatgttctg agcgaagatt tcgttttcta
     7921 ttatcagaat catgccgatc gtctgggcat tgaaggcagc attcgtcgta ttgtctatga
     7981 acacgatctc acgctgaaag ataagctctt ttcgtcactc ttagatcagc ccgcgcaggc
     8041 agcgctgtgg cacgataaac aaggccatct ttcacattat atggtgctga tccagcgcag
     8101 tggtttaagc aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag
     8161 caaaacggaa atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg
     8221 ctataacggc aataacgccc tgatgttcgc gacatatcag gacaagatgc tggtgttttc
     8281 cagcacggat atgctgttta aagatgatca gcaggatacc gaagccacgg cgatcgcaag
     8341 tgatttgttg agcggcaaaa aacgctggca agccagcttt ggcctggaag agcgtgctgc
     8401 cgaaaaaacg ccagtacgcc agcgcatcgt ggccagcgcc aggttgctgg gttttggcta
     8461 ccagcgatta atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca
     8521 cagttttgtg gcgttaaatg atgaatccgc cagcgtagat gccagtttcg attttacgcc
     8581 ggtctggaac agtatgcctg ccggggccag cttctgtgtg gcggtgccgt attcacacgg
     8641 tattgccgaa gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt
     8701 agacggtgcc gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt
     8761 cggtcagttt gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa
     8821 tattggtgcg cacgaaagca aagcgccaga aggtgttttg ccggtaagcc agactcagca
     8881 gggcgaagcg caaatctggc gtcgcgaagt gagttcccga tacggccagt atccgaaagc
     8941 gcaggcggcg caacccgatc aattaatgtc ggattatttt ttccgtgtgt cgctggcgat
     9001 gcaaaacaaa acgctgcttt tctccctcga tgacacgctg gttaataacg cgctgcaaac
     9061 actgaataaa acccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct
     9121 ctatatcaat ccacaaggaa tagcgaaact gctgcgtaac gaaacgttga ccagtctgcc
     9181 gaagaatctc gaaccggttt tttataacgc cgcacaaact ttattaatgc cgaagctgga
     9241 cgctttatct caacaaccgc gttatgtcat gaagctggcc cagatggaac ccggtgccgc
     9301 ctggcagtgg ctacccataa cctggcagcc gctatgaggc acgggctgct ggcgctgatt
     9361 tgctggctgt gttgtgttgt tgcccatagc gaaatgctga atgtcgaaca atccgggctg
     9421 tttcgcgcct ggtttgtacg cattgcacaa gaacagctcc gccaggggcc aagtccacgc
     9481 tggtatcagc aggattgtgc gggcctggtg cgatttgcag cgaacgaggc gctgaaagtt
     9541 cacgacagca aatggctaaa aagtaacggc atagcaagtc agtatttgcc gcctgaaatg
     9601 acgttaaaac ctgaacagcg tcaactggcg caaaactgga atcaggggaa cgggaaaacc
     9661 ggtccctatg tgaccgcgat taatttgatt cagtacaaca gccagtttat tggccaggac
     9721 ataaaccagg cgctgcctgg cgatatgatt tttttcgatc agggcgatgc ccagcactta
     9781 atggtctgga tggggcgtta cgtcatctac cacaccggaa gcgccacgaa aactgacaac
     9841 ggaatgcgcg cagtcagtct gcaacaactt atgacatgga aggacacccg atggatactc
     9901 aacgattcca atcccaattt cattggcatt tatcgtttaa attttctggc gcgatagcag
     9961 cgtgcttgtc cctgtctctt gtgggaacag ggcttgctaa tgctgatgat tcgcttcctt
    10021 ccagtaacta tgcgccgccc gcagggggaa cattcttttt gcttgctgac agcagtttta
    10081 gcagcagtga agaggcgaaa gtgcgactgg aagcgccggg gcgtgattat cggcgctatc
    10141 agatggaaga gtacggcggc gtggacgttc gcctgtatcg tattcctgac ccgatggcat
    10201 ttttgcgcca gcagaaaaac ctgcatcgca ttgtggtgca accgcaatat ttgggcgacg
    10261 ggctgaacaa tacgctaacc tggctgtggg ataactggta cggcaaatct cgccgcgtga
    10321 tgcagcgtac tttctcttct cagtcaaggc agaatgtgac tcaggcgtta cccgaattac
    10381 agctcggcaa tgccattatt aaaccttccc gttatgtaca gaacaaccag ttttccccgc
    10441 tgaaaaaata tcccctggtg gaacagttcc gttatccact atggcaggct aaaccgttcg
    10501 agccgcagca aggggtaaaa ctggaaggcg catccagcaa tttcatctcg ccgcagccgg
    10561 gtaacattta tattcctctc ggccaacaag agccgggact gtacctcgtc gaggcgatgg
    10621 ttggtgggta tcgggcgacg acggtggtgt ttgtttccga taccgtggcg cttagcaaag
    10681 tgtcaggcaa agagcttctg gtgtggaccg cgggtaaaaa acagggtgaa gcgaagcccg
    10741 gctcagagat cttgtggact gacggtcttg gcgtgatgac ccgcggtgtg accgatgaca
    10801 gcggtacctt gcagttacaa catatatcgc cagaacgttc atacattctg ggtaaggatg
    10861 ctgaaggcgg cgtttttgtc tccgagaact tcttctacga aagcgaaatc tacaacaccc
    10921 gcttgtatat ttttaccgat cgcccgctat atcgcgcagg cgatcgtgtc gatgttaaag
    10981 tgatcggccg cgagttccac gatccgttgc attcatcccc catcgtcagc gccccggcga
    11041 agctttcggt gctggacgcc aacggcagtc tgttgcaaac cgtcaatgtc acgctggatg
    11101 cgcgcaatgg cgggcaggga agtttccgcc tgccagaaaa tgccgtagcc ggaggttatg
    11161 agttacgtct tgcttaccgc aatcaggtct atagcagcag ttttcgcgtg gcaaactaca
    11221 tcaagccaca tttcgagatt ggtttagctc tcgacaaaaa agagttcaaa actggcgaag
    11281 cggtcagcgg caaactgcaa ctgctctacc cggatggcga gccggtaaaa aatgcccgcg
    11341 tgcagttaag tttgcgcgct cagcaattat caatggtcgg taacgatttg cgttatgccg
    11401 gacgtttccc cgtgtcgctg gaaggcagcg aaacggtgtc cgacgccagt ggtcatgtga
    11461 cgttaaatct ccccgccgcc gataaaccga gccgctattt gttaaccgtc tccgccagtg
    11521 acggcgcggc gtatcgcgtc accaccacca aagagatcct cattgaacgc ggcctggcgc
    11581 attactcttt aagtaccgcc gcacaataca gtaatagcgg cgagtcggtt gtgttccgtt
    11641 atgccgcgct ggaatcttca aaacaggttc ctgttacgta tgaatggttg cgtctcgaag
    11701 accgcacgag ccatagcgga gagctaccgt caggcggcaa atcctttacc gtcaatttcg
    11761 ctaaacctgg caactacaat ctgacattac gcgataaaga cggcttaatt ctcgctgggt
    11821 taagtcatgc cgtcagcggt aagggcagca cggcgcatac tggtacggta gatatcgtgg
    11881 cggataaaac gctgtaccag ccaggcgaaa ccgcgaagat gctgattacc tttccggagc
    11941 caattgatga agcattattg acgctggaac gcgatcgcgt ggaacagcag tcgctgcttt
    12001 cgcatccggc aaactggcta acgctacaac gtttaaacga tacccagtat gaagcccggg
    12061 ttcgagtgag caattccttt gcgcctaaca tcactttttc ggtgctgtat acccgtaatg
    12121 gtcagtacag ttttcagaac gccgggatca aagttgccgt tcctcagctt gatatccggg
    12181 tgaaaacgga caaaacccat taccagcctg gtgaactggt caatgtcgaa ttaacctcgt
    12241 cgctgaaagg taaacctgtt tctgcgcagc taacggtagg cgtggtcgat gaaatgatct
    12301 acgcgctgca accagaaatc gcgccgaata tcggcaaatt tttctatccg ctggggcgta
    12361 acaatgtgcg taccagctcc agtctgtcgt ttatcagcta cgaccaggcg ctctccagcg
    12421 agccggttgc gcctggcgcg actaaccgca gcgagcggcg agtaaaaatg cttgaacgtc
    12481 cacggcgtga agaggtggat accgcggcat ggatgccgtc actcacaacc gataaacaag
    12541 gcaaagcgta tttcacgttc ctgatgcctg attcgttaac ccgctggcgt atcaccgcgc
    12601 gtgggatgaa cggcgacggg ctggtcgggc aggggcgtgc ttatctgcgt tcggaaaaaa
    12661 atctctacat gaagtggagt atgccaacgg tgtatcgcgt gggcgacaaa ccggcggcag
    12721 gactgtttat cttcagtcag caggataacg aaccggtggc gctggtgact aaatttgcag
    12781 gcgctgagat gcgccagacg ctgacgctgc acaaaggggc gaattatatt tcgctgacgc
    12841 agaatattca gcaatctggc ttgttaagtg cagaactgca acaaaatggg caagtgcagg
    12901 acagcattag cacaaaactg tcttttgtgg ataacagctg gcccgttgaa cagcagaaaa
    12961 atgtcatgct cggtggtggc gataacgcgc tgatgttgcc cgagcaggcg agcaatatcc
    13021 ggctacaaag tagtgaaacg ccgcaggaga ttttccgcaa caatcttgat gcgttagtcg
    13081 atgaaccgtg gggtggcgta atcaacaccg gtagccgtct gatcccgctc agtctcgcct
    13141 ggcgttcgct tgccgatcat caaagtgccg ccgctaacga cattcgtcag atgattcagg
    13201 ataaccgtct gcggctgatg caactggcgg ggcccggagc gcgctttacc tggtggggtg
    13261 aagatggcaa tggtgacgcc ttccttacgg catgggcatg gtacgccgac tggcaggcca
    13321 gccaggcgat cggcgtaacg caacaaccgg aatactggca gcatatgctc gacagttatg
    13381 ccgagcaggc agataacgtg ccgttattgc atcgggcgct ggtgctggcg tgggcacagg
    13441 agatgaatct gccgtgcaaa acgttgttga aagggttgga tgaagctatc gcccggcgcg
    13501 gaactaaaac tgaagatttc tctgaggaag accccagcga tatcaatgac agcctgatct
    13561 tcgatacacc ggaatctcca ctggcagatg cggtggcaaa cgtcttaacc atgacgttgc
    13621 tgaaaaaagc gcagttgaag tccacggtga tgccacaggt tcagcaatat gcgagggata
    13681 aagcggcaaa cagcaatcag ccgctggcgc acacggttgt gctgcttaat agcggtggcg
    13741 acgctaccca gacggccgct attttaagtg gtttgaccgc tgagcaatcc actattgaac
    13801 gcgcgctggc catgaactgg ctggcgaaat atatggcgac aatgcctccg gttgtgttgc
    13861 ctgcgcctgc gggcgcatgg gccaaacata agttaactgg agggggcgaa tactggcgtt
    13921 gggttggtca gggtgtgccg gacattctct cttttggtga cgaattatcg ccgcaaaatg
    13981 tgcaggtccg ctggcgtgaa ccggcaaaaa cggctcaaca aagtaacatt ccggtaaccg
    14041 ttgaacgcca gttgtatcgg cttatccctg gtgaagaaga gatgagcttt actctgcaac
    14101 cggtgaccag caatgagatt gacagcgatg cgctgtatct cgatgaaatc acgcttacca
    14161 gcgagcagga tgcagttctg cgctacggtc aggtggaagt accgctccca ccgggagccg
    14221 acgttgagcg cacaacatgg ggcatttcgg tcaataaacc caacgccgcg aaacaacagg
    14281 ggcaattgct ggaaaaagca cgtaatgaaa tgggcgaact ggcctatatg gtgccggtga
    14341 aagaactgac gggaacggtc actttccgcc atttgctgcg cttctcgcaa aaagggcaat
    14401 tcgttctgcc tcctgctcgt tatgtgcgtt cctatgcacc tgcacagcaa agtgttgcgg
    14461 cagggagtga atggaccggg atgcaggtga aataagtgaa ctggcgcaga atcgtctggc
    14521 tattggcgtt ggttactctg ccaacgcttg cggaggagac gccgttacaa ctggcgctgc
    14581 ggggcgcgca gcacgatcaa ctgtatcagt tgtcgtcatc aggcgtgacc aaagtctcgg
    14641 cattacctga cacgctcacc acgccgttag ggagtttgtg gaaactgtat gtttacgcca
    14701 ggctggagga tacccatcaa ccggagcagc cctaccagtg tcgcggaaac tccccggaag
    14761 aggtttattg ttgtcaggct ggggagagca taacgcgcga taccgcgctg gtacgttcct
    14821 gtgggctcta ttttgcgccg cagcggttgc atattggcgc agatgtgtgg gggcaatact
    14881 ggcaacagcg tcaggcacct gcgtggttag cgtctcttac aacgctgaag ccagaaacct
    14941 cggtaacggt taaatcgctg ctcgattcat tagcgacgtt acctgcgcaa aacaaagctc
    15001 aggaagtctt actggatgtg gtgctggacg aggcgaaaat cggtgtcgca tccatgctcg
    15061 gtagtcgggt acgggtgaaa acctggagct ggtttgccga tgataaacag gaaatccgcc
    15121 agggtggttt tgctggctgg ttaaccgatg gtacaccgct gtgggttact ggcagtggca
    15181 ccagcaaaac ggtactaacc cgttatgcga cggtgcttaa tcgggttttg ccagtgccga
    15241 cgcaggttgc cagtgggcaa tgtgtagagg tggagctgtt tgcccgctat ccgctgaaga
    15301 aaatcacagc ggaaaagagt acgacggcag taaaacccgg cgtacttaat gggcgctatc
    15361 gtgtgacgtt tactaatggt aaccacatta cgtttgtcag ccacggtgag acgaccttac
    15421 tgagcgaaaa gggcaaactg aaattgcagt cacatctcga tcgtgaagag tacgttgcca
    15481 gagttctgga tcgcgaggcg aaaagtacgc cccctgaggc agcgaaagcc atgaccgtgg
    15541 cgattcgcac ttttttacag caaaacgcca atcgggaagg cgattgcctg accatccctg
    15601 acagcagcgc cacgcagcgt gtttcggctt cgccagctac gacgggggcg cgaacgatgg
    15661 ccgcctggac gcaggatctc atctatgcgg gcgatccggt tcattatcat ggcagtcgcg
    15721 ctaccgaagg gacgctttcc tggcggcaag ccacggccca ggcggggcag ggagagcgtt
    15781 acgaccagat actcgcgttt gcttatcccg acaacagcct tagccgctgg ggagcgccac
    15841 gctcaacctg ccagttatta cccaaagcga aagcctggct ggcgaaaaaa aagccgcagt
    15901 ggcggcgtat attacaagct gagacggggt acaacgaacc agacgtgttt gcggtttgcc
    15961 gtttagtctc aggtttcccc tataccgatc ggcagcagaa acggctgttt attcgtaact
    16021 tctttacgct tcaggatcgg ctggatttaa cccatgagta tctgcacctg gcctttgatg
    16081 gttatcccac cgggcttgat gagaactata tcgaaacgct gacccgacaa ttattaatgg
    16141 actaatgcta tgcgaaagat ttttcttccg ttgttactgg tggcactttc gcctgtcgtt
    16201 cacagtgaag gtgtgcagga agtcgagatt gacgcaccgc tctctggctg gcatccggta
    16261 gagggcgaag atgccagttt ttcacaatcg atcaattacc cggcgtcgtc cgtcaacatg
    16321 gcagatgatc aaaatatctc agcgcagatc cgcggcaaaa taaagaatta tgctgcggcg
    16381 ggtaaagttc agcagggccg gctggtggtc aacggtgcca gtatgccgca gagaattgaa
    16441 tccgatggtt catttgcacg cccttatatt ttcactgaag gcagcaacag cgtgcaggtc
    16501 atcagcccgg atgggcaaag ccgacaaaaa atgcagtttt actcaacgcc gggtacagga
    16561 acgattcgtg cacgtttacg gctggttctc tcgtgggata cggacaatac cgatctcgat
    16621 ctccatgttg tcacgcccga tggcgaacac gcctggtacg gtaacacggt gctgaaaaac
    16681 agtggtgcac tggatatgga tgtcacgacg gggtacggac ccgagatttt cgccatgcca
    16741 gcgccagttc acggacgcta tcaggtgtat atcaactact atggcggacg cagcgaaacg
    16801 gaattaacca ccgcccaact gacgctgatc accgatgaag gatcggtcaa tgagaaacag
    16861 gaaacattta ttgtaccgat gcgtaatgct ggcgagctga cgctggtgaa aagttttgac
    16921 tggtgattaa tggggttacc ttcgctgtcg atgttcaaga aaacgcccga taactttcgc
    16981 tatcgggcgt ttttgttcgt taattcaacc gttcaatcac catcgcaatt ccctggccgc
    17041 caccaatgca cagtgttgcc agccccagcg ttttatcgcg tgcctgcatg gcatgtaata
    17101 gtgtgaccag aatacgagca ccactggcac cgataggatg cccgagcgcg atggccccgc
    17161 cgttgacatt cactttctca ggatcaaagc ccaaggtttt cccaacggca aggaactgtg
    17221 cagcaaatgc ttcattagct tcgatgagat caatgtctgc cagttgcagc cccgccagtt
    17281 gtaacgcttt ttgcgtggca ggtactggcc ccatacccat caatgcggga ggcacgccac
    17341 cgctggcata acttttaatg cgagccaggg gggtaaggcc tgctgccagc gccgcagatt
    17401 cttccataat caccagagcg gcagcaccgt cgttaatacc agacgcgttc ccagcggtga
    17461 ctgttcctgc tttatcgaag gccgggcgca atgcacctaa cgcttcagcc gttgaattcg
    17521 ctttcgggaa ttcgtcttga ctgaagacga aggttttctt ccgagtgaca acatttaccg
    17581 ggacgatttc ggctgtaaaa gcaccggact caattgcggc tgccgcttta cgctgtgaat
    17641 gtagcgccag ttcatcctgc atttcacggg taattccgta ctctttagcc acgttttcgg
    17701 cggtaatccc catatgataa ccatgggtgg cgcacatcag gccatcgcgc aggattacgt
    17761 cataaacctg tccgtctcca agacgataac cagagcgtgc ttttgcatcg agtaagtagg
    17821 gcgctaaact catattttcc ataccccccg ccacaatgct ctgcgcctga cctgcctgaa
    17881 ttgcctgggc ggcaagcgcc acacttttaa gacccgaacc acacacttta ttgaccgtga
    17941 atccgcacac cgtttctgcc agcccgcttt ttaacagtgc ctgacgtgcc ggattttgcc
    18001 ccaacccggc ttgcaacacg ttacccataa tcacttcatc aatgtgtagt gaatcgattt
    18061 ttgcacgttc aatggcggct ttaattactg tcgcccccag gtcgatggcg ctggtggaag
    18121 cgagtgaacc gttaaaacta ccgatagcag tacgtaccgc actgacgatg acacaatttt
    18181 tcattttata ttccttctgt ctgtagggtt gccgtcagaa cagcgttaaa ccaatgacga
    18241 aaatgacgcc ggagaagagc agggcagtga tgcaatagcc catgatgtcg cggacaccga
    18301 gtccggcgat tgccagtgct ggcagcgccc agaatggttg tgccatgttc atccattgct
    18361 cgccgtaggc gatcgccatt accgatttac cgagatcagc gcccagcgcc tgggctgcgg
    18421 gtatcacgaa aggtccctga ataacccagt gaccgccgcc agacggaacg gcgaagttaa
    18481 tcagtgcaga actaaaaaag gtcattaccg ggaaggtgtc tttgttcgca acattgatga
    18541 agaattcggt aatgagtcca cccagaccag aatgctccat catcagttgg atcccagcgt
    18601 agaaggggaa ttgcaccaga ataccggcag tactgcgtgc tgccgcgctg atagcacgca
    18661 tataagccat tggcgtttta tgtagcaaca gacccgcaat cataaacatc aggttgacgg
    18721 tattgatggt gatgttgaag ccatgttcgc tgaagtacat cgccaggtaa gcgataccga
    18781 gtgcgccgat gatcaacgtc agaatgcggc tttcttccag tcgctcggat ggtggggcat
    18841 ctttcggtag ctgcttttga aaatcagcct cttccatgag tagtttcgga tcgatactca
    18901 ccacgtcaga cggttttggc atcatcatgc gggtgataaa tggcatcacc acaatcaacg
    18961 tcacagtgat gaaaatgtta aaaccactga acagagtatc gcccaccggg atcagcccgg
    19021 cgatatgctc aaccgggttg cccggtgttg cagccaacag aggcattgat ccagagaagc
    19081 cgccacccca ggtgagaaaa ccaatgtagg cgcaggcaat gagcaacgga taatcagaac
    19141 cggggactcg ccgggcgact tcacgggcaa acattgcgcc gacaaccaaa ccaaatcccc
    19201 agttgatgac acaagcgact gaaccgaaga aagtgaccag catgacgccc tgtacgggcg
    19261 tctttacggc ggaggcggca gtacgcagca aacttttcac cggagcagag ctggcaaggg
    19321 catgaccggt aacgatgata agcgccatct gcataccaaa cgccagcaag ttccagaaac
    19381 cgtcacccca cattttcacc atgctgatcg gcgtttgtgg tgttaaccaa agcgcgatca
    19441 cgaatgttag caatgtcagc aacatggcaa agatcagtgg atcgggaagc caccggctga
    19501 caaaacgcgt cataaaacgc gatatgcgac caatcataaa tcaccccgtt gcgtattcag
    19561 atcggcggcg acttcaaacc gagcttctgt tttggcacgc acggtggcta aatcacaccc
    19621 gtcggcaatt tcggtgagcc acattttgcc gtcaataaaa cgaaagacag ccagttcagt
    19681 aaccagcata tgcaccgcat gttgcgcagt gagtggcatg gtgcagcggc gcaaaatttt
    19741 tgctgaacca tctttggcgc aatgttccat ggcgatgatc actttgcgcg acccggtcac
    19801 cagatccatc gcgccaccca taccgggcac cattttccca ggcactaccc agttcgcgag
    19861 gtttgcttct tcgtctactt gcaaaccgcc gagcacgcag gcatcaatat gaccgccacg
    19921 gattagcgca aatgacatgg cgctatcaaa catggctgca ccgggtaaaa caccgcacgg
    19981 ttgcccgcca gcgttcacca gatctggatg cgctgtcgtg accgggccta aaccgaggaa
    20041 gccgttttcc gattgcagag tgatatgaat accctccggt aaataattgg cgaccattgt
    20101 gggtaaaccg atccctaagt taacgatgtc accatcacga agctcttgcg ccacacggcg
    20161 cgcaatacgt tgtttcgcat ccattatttg ctctcctgtg aaacgatgat gtggtcgata
    20221 acggcaccag gggtgacaat atggtcaggt tgcagctcgc cggtttcgac cagttcatct
    20281 ggctctacca gcgtgatatc agccgcaagg gctatcaggg ggttaaagtt gcgggcgcta
    20341 agttgatagg tcaggttgcc aagtgtgtcg caacgatgag cgcgaattag cgccaggtcg
    20401 gcgcgcagtg ggcgttcgag cagccaagtt ttaccgtcga gtgtcagtgt ctgtttgcct
    20461 tcctctacga cggtgccgac acccgttggg gtgagaaaac caccaagtcc agctccacca
    20521 cagcgaattt gctcgattag cgtaccttgc ggcaccagaa cgacgtccat ctcaccagat
    20581 atcatgcgcc gacctgtttc cgggttggtg ccgatatgtg aagcaatcac tttgcggact
    20641 cgaccattga cgatgagcgg accgatgccg gtatcaacaa acgcggtatc attggctatc
    20701 aatgtcaggt cgcgaacacc agattccagt aatgcttcaa ccaggcggga tggagtgcca
    20761 atccccataa atccgcccac catgatggtc atgccgtcac gaaagaagcc ggtggcgtct
    20821 tgtaatgtca tcaattttgt tttcattttt tatccctctt gcatacaccg ttgtgggtac
    20881 agtcaggcaa tagcaagggg agtgccagaa tgagattatt ggatttagtt atttaactaa
    20941 ttgaatatga agggataaat tggatgggga tttataagga gaatgagcgg ctatgcagaa
    21001 aattgcgcac tgtgcaaatt tctgcatagc aagttttggt gttatacatc cgccggatcg
    21061 ataccgtatt cctggagttt atacatcaat gcacggcgac tgatgcccag cattaaagca
    21121 gtgcgggttc ggtttccttc ttgttgttcc agcacttcca taatgatgcg tttttcgacg
    21181 cgtttaattt cctcttttaa attacgctca ccgacagggg ctgtttttac ctcgccagca
    21241 ttacagactg gctgacgaat ctgtggcgga agatcctcag aaaaaatgat cgggcctgaa
    21301 ttcatcacga cggcgcgttc aataacgttg gaaagctctc gaatatttcc cggccatgac
    21361 caggcggtaa gcagtgacat tgccatcgga tcgatgtcga taatatcgcg ctgattctca
    21421 ctactgaatt tttgcaaaaa gtgattagct aacagggaaa tatcttcccg gcgatcgcgc
    21481 agaggcggca gtattaaatg aataacgtta aggcgataaa agagatcttc acggaaggta
    21541 ccttctttta ccattgcctg caagtcgcgg ttggtggcag caatgatgcg gatatcaact
    21601 tttatggtct gatggccgcc aatccgttca aattcccgtt cctgtagaat gcgtagtaat
    21661 ttggcttgta gtaccagcgg catttcgcca atttcgtcga ggagcagagt accttcgttg
    21721 gctcgttcaa ataatccctg acgcaaggtt tgtgcaccag taaatgcacc tttttcatga
    21781 ccaaacagtt cactttcgag caacgattcc ggcagcgccg cgcagttgac tttaatgaac
    21841 ggcccctttg cccgccgcga attgtagtga atcgctctgg caatcaactc tttcccggtg
    21901 ccgctttcac cgctaatcaa gacgctggcc tgagaaaggg caattttggc ggtgtctttg
    21961 cagatgtcca tcatcgccgg gctgttggtg agaatgtgcc cccattgcca gctggtgctc
    22021 agtgcctggt gcagatgacg gatctctttt ttcattgact ggagttgtaa agcgcgctga
    22081 acgattaaat tcaactcatc gagatcaaac ggtttaataa catagtcgaa ggctccgcag
    22141 cgtagcgctt cgacggcggt ttccacttcc gcataggccg tcatcagaat aacgggtgtc
    22201 cgggtctcat ggctgcgcat ctcctttagt gccttgatgc cgtccatctc tggcatgcgg
    22261 atatccatca acaccacatc agggtgaata tcggcaaaca ggtgtaatgc tgtgcgtccg
    22321 ttgttcgcac aatgtgtttc gaatccttgt agtgcaaaag cggtgctcag catacggcga
    22381 acattatctt catcatccac aataaggatg cgattaatag cagtcataca gtctgatttc
    22441 cctgcgggtt gatcggtaaa ataagcgtga aggttgcgcc gtagcccggc aaactggcga
    22501 cgcgaatatc accctgatgg gcattaatga tgcgttgact taacgccaga ccaagcccgg
    22561 ttcctgaggc tttggtggtg aaaaagggat cgaagatctt tttttgcagc gagagatcaa
    22621 tgccacagcc gttgtcctct atcgaaatgg cctgttgtga gtcgctgtat tgccaggttt
    22681 gaatgcgaat tttccctcgt gcgctgatag cctggacggc attgatcagg atattcagta
    22741 gtacctgttt gagcagttca cgatcggcgt taatcgggct taattcatta tccagttcgc
    22801 ttatgaagtc gacccgcgct tgtacgccgg cggtttgtac cagtaccaga gtttcttcaa
    22861 ccaatgcatt gaggctgact tgttgccatt gactgtggcg tggacgtgaa aattcgagca
    22921 attgctgaat aactttgtta attgaatcga tttctttgag tactacggac agatattcct
    22981 gatgtattgg gtcactggtt tgttggcgca agatctgtac ataaccacga atagccgtta
    23041 acggattacg tacttcatgc gcgacgccag ccatcagctc acccagtgtg gcgaggcgtt
    23101 ctgcttgcgc catgcggcgc tgggtttctt tgcgggcagt taaatcagag aaaatcacca
    23161 aagcacctat catttcaccg tgcgtgttat gaatacgact ggtagtgaca ctgagttcaa
    23221 tggtgcggtc acgacctgga aaactgatct ccagcgccac atgttcggtg ccatgttcca
    23281 gcgtatccag tactggactg tagaactgag tattgtcgaa caacatggag taaggctgcc
    23341 ctaccagttc atggcgttga tagccagtga taacttctgc tgctgggttc atggtggtta
    23401 catcaccctg gcggtcaatg gcaatgacgc catcggcagc gttttcaata atcagatcgt
    23461 taagtgtccg cgtttcacgc agtgcctggg cgaggttatt aacactctga ctgatttgcc
    23521 ccatttcacc gggcaattgt ggtaatcgag tgggaatatt ttgtgccaga gtcgagaggc
    23581 catcggtgat gatatcaata ttggcgctca ggcgacggga gaaaaggaca atcaatagca
    23641 ggcttatcag caaaccggcg gtgagcacaa tgataatcct cacatccatt ttccaggcct
    23701 ggcggcgaat atcttcggtt aattcattgg cccagatata gccgaggatt tcaccattac
    23761 gctcaatggg gagcattgaa ttcaaaatat cgccgcgcac ctgcctgcct gaataaacca
    23821 aaggggtatt tgtacgcatg acttcgcgac cagggtgatc tgcggcaatg gtaacgccga
    23881 cattattctg atatagcgct gaaggcgcgt aggtgattat cgcatccagc attttgttgt
    23941 aataaccagc accgatgcca gggaaggcgt gagtgatatt ttcggtaatg ggggcaagtt
    24001 ctgcatttaa tgcgcggata cgctcctcac gtggtaagtc gatgtagaga tcatagcgat
    24061 cgcctagtgc ctgattaagc aggttgacca cggcagaaag ttttttctct ttttcagata
    24121 agactgctga acgtccttcc gtttctacga tataaccaat agtaagcgtt gggacaatga
    24181 ccatcaggat tgccatcagg atcatttgat tgcgtaagcg gcgtggataa atccacttca
    24241 tataatgcat gctgaaatcc actagtcttg tccggtatat gacgattatc agaggttaag
    24301 gtgatgattt ctcggcggtg tatcatattc cagagaagag agaacattgc ggtaacacgc
    24361 ttttaccgct accttaacca cactccatcg gtcacctgag gcggagcttc gcccctttga
    24421 aataccttgc ttcttttcgt acaaccctga aagcctcgcg ctacatgttc agagcattgg
    24481 cgttagtgct ctggctgttg attgcttttt catccgtttt ttacatcgtt aatgcgttac
    24541 atcagcgaga atcggaaatt cgtcaggaat ttaatctgag ttccgatcag gctcagcgct
    24601 ttattcaacg cacctctgat gtgatgaaag agctgaagta catcgccgaa aatcgcttat
    24661 cggcagaaaa cggtgtgctt tccccgcgtg gacgagaaac gcaggcggat gtgcctgcgt
    24721 ttgaaccgct gtttgccgac tccgattgtt ccgcaatgag taacacctgg cgaggttctc
    24781 tggagtcatt ggcgtggttt atgcgctact ggcgcgataa tttttctgcg gcttacgatc
    24841 taaaccgggt atttttaatc ggcagcgata acctctgcat ggccaatttc ggtctgcgtg
    24901 atatgccagt ggaacgcgat accgcgttga aagctttgca tgaacgcatc aataaatatc
    24961 gaaatgcacc acaagatgat agcggcagta acctctactg gatcagcgaa ggtccgcgcc
    25021 ctggcgtcgg gtatttttac gcgttgacgc cagtttatct ggcgaaccgg ttgcaggcgc
    25081 ttttgggtgt cgagcagacc atccggatgg agaacttttt cttaccgggt acgttgccga
    25141 tgggggttac cattcttgat gaaaatggtc ataccctgat ttcgcttacc ggaccagaaa
    25201 gtaaaattaa gggcgatcct cgctggatgc aggaacgctc ctggtttggc tatacggaag
    25261 ggttccggga gctggtgctg aagaaaaatc tgccaccctc atcgctaagc atcgtgtatt
    25321 cggtgccggt tgataaggtg ctggaacgca ttcgcatgtt gatccttaac gcaattttgc
    25381 tgaatgtgct tgccggagct gcattgttta ctctcgcacg gatgtacgag cgacgtattt
    25441 tcattccggc ggaaagcgac gccctgcgac tggaagaaca tgagcagttc aatcgcaaga
    25501 ttgtcgcctc cgcgccagtg ggtatctgca ttttgcgtac cgctgatggc gtcaatattt
    25561 taagtaacga actggcgcat acctatctca atatgcttac gcatgaggac cgccaacgac
    25621 tgacgcaaat tatctgtggg cagcaggtca attttgttga tgtcctgacc agcaacaata
    25681 ccaatctgca aatcagcttc gtccattcgc gctatcgtaa tgaaaacgtg gccatttgtg
    25741 tgctggtgga tgtttcttcg cgcgtgaaga tggaagagtc gttgcaggag atggcacaag
    25801 cagcggaaca ggcgagccag tcaaaatcga tgttccttgc caccgtcagt catgagctgc
    25861 gaacgccgct gtatggcatt atcggtaacc tggatctgtt gcaaaccaaa gagttaccga
    25921 aaggcgtcga tcggctggtg acggcaatga acaactcttc cagcctgttg ttgaaaatta
    25981 tcagcgatat tctcgatttc tcgaagattg aatcggaaca gttgaagatc gaaccgcgtg
    26041 agttttcacc gcgtgaagtg atgaaccaca tcaccgccaa ctatttaccg ctggtggtac
    26101 gcaagcagct aggcttgtac tgctttattg aaccggatgt gccagtggcc ttaaatggcg
    26161 acccgatgcg tttacagcag gtcatctcca acctgttgag taacgccata aaattcaccg
    26221 ataccggctg tatagttttg catgtccgcg ctgatggcga ttatctctct atccgtgttc
    26281 gcgataccgg cgtggggatt ccggcgaaag aagtggtgcg cttgtttgat cccttcttcc
    26341 aggtcggaac gggcgtacag cgaaacttcc aggggaccgg tctgggtctg gcgatttgtg
    26401 aaaaactgat cagcatgatg gacggcgata tctcggtaga ttcagaaccg ggaatgggca
    26461 gccagtttac cgtgcgtatt ccgttgtacg gcgctcagta cccgcagaaa aaaggcgtgg
    26521 aagggttgag tggtaaacgc tgctggctgg cggtccgcaa tgcgtcgctc tgtcagttcc
    26581 tggaaaccag tttgcagcgc agcggcatcg tcgttacaac atacgaaggg caggaaccga
    26641 ctcccgaaga tgtgttaatt actgacgagg tagtgagtaa aaaatggcag ggtagagcgg
    26701 tagtgacctt ctgtcgtcgc catattggta ttccgctgga gaaagcgcca ggggagtggg
    26761 tacacagtgt ggctgccccg catgagctac cggcattgtt ggcgcgtatt tatttgatcg
    26821 agatggagag cgacgatcct gctaacgctc tgccgtcgac ggacaaagcg gtcagcgata
    26881 atgacgatat gatgattctg gtcgtggatg atcatccgat taaccgacgt ctgctggcag
    26941 atcagttggg atcgttgggc tatcaatgta aaaccgcgaa tgatggtgtc gatgcgctta
    27001 atgtacttag caagaatcat attgatatcg tgcttagcga cgtcaacatg ccaaatatgg
    27061 atggttaccg cttgacgcaa cgcattcgtc agttgggact gacgttgccg gtaatcggag
    27121 taactgctaa tgcgttggct gaagagaagc agcggtgtct ggagtccggt atggacagct
    27181 gcctgtcgaa gccggtaacg ctggatgtga taaaacagac gctgacggta tatgccgaga
    27241 gggtcaggaa atcgcgggaa tcgtaggacg gataaggtgt ttatgccgca tctggcattc
    27301 agtgcaaatg ccagatgcga cgctgacgcg tcttatctgg cctactttaa tgtaatctgt
    27361 tgaaataatg ggaatcgtag gccggataag gcgtttacgc tgcatccggc aatcggtgca
    27421 aatgccagat aagacactaa cgcgtcttat ctggcctaca gatgattagt ctttatctgc
    27481 cggacttaag gtcacagaag agagataatt cagcagggcg atatcgttct cgacacccag
    27541 cttcatcatc gcagatttct tctggctact gatggtttta atactgcggt tcagcttttt
    27601 agcgatctcg gtcaccagga agccttccgc aaacaggcgc agaacttcac tctcttttgg
    27661 cgagagacgc ttgtcaccgt aaccaccagc actgattttt tccaacaggc gagaaacgct
    27721 ttccggggta aatttcttcc ctttctgcag cgcggcgaga gctttcggca gatcggtcgg
    27781 tgcaccttgt ttcagcacga tcccttcgat atccagatcc aataccgcac taagaatcgc
    27841 cgggttgttg ttcatagtca gaacaatgat cgacaggctt gggaaatggc gcttgatgta
    27901 cttgattaag gtaatgccat cgccgtactt atcgccaggc atggagagat cggtaatcaa
    27961 cacatgcgca tccagtttcg gcaggttgtt gatcagtgct gtagagtctt caaattcgcc
    28021 gacaacattc acccactcaa tttgctcaag tgatttgcga ataccgaaca agactatcgg
    28081 atggtcatcg gcaataatta cgttcatatt gttcatgtaa taggctacct tgctacagca
    28141 agctcttgac ataactgtca atgtcgctga tgtatttttc tattcctgga acatccttct
    28201 cacgaatcag atgttccagc gtttcacata actgcttgcc gggtaccaga tttagcatgg
    28261 caaatacgcc tttaagacga tgagccgttt gggctaacgc agcaaagtca ctggttgctg
    28321 cttcagtata cagcctctta acatcatccg gtactgtgtc tacaaagagc gcataatagc
    28381 cgctggcatg gagttgcgca ttttcatctc cgcccagagg cgattctgtc acctcttcct
    28441 gcgccagttg cacttcaatt aattgtaaga ccgcttcctg catagcgttg ctcatattga
    28501 agttgacgca caattgacca ggcccaattt cccgtacgcc agactcatca tcgcttaaaa
    28561 gcaagccaga ggcagtaaga ttagacggat tatccgttaa aaagatatca taatcttgac
    28621 taattaatct ttcatcgggt gtgatacagg ttgcacccca attttctaac tggcgagtga
    28681 caatattccg aatttctgcc gaagtaacat ccaccattac gcagacatca tccagtaaac
    28741 gctcttcttc ctcttcaact tccgggtcag cggcgagcat tttgatatgc acagagtagc
    28801 gtgtaccaag cccatcccgc gttttgatgt ttaaatgacc gcccagttta cgtgccagtt
    28861 gatcgctcag ccagaatgcc agcgggtccg cctttccata acgatcgttt tgggtctggt
    28921 tgataaacgg gaagtgcaaa ttatccattt catgaatact tacgccttct cccgtgtcca
    28981 gaatgcgaaa cgtcaggcgg tcttcggagg actcatcctg atcaacctca agggtgattt
    29041 ttcccaattg cgttgaggtc acggcatatt gcatcagtag cagcaaaata cgtcgtaagg
    29101 catcgcgatc gccacggcgc atatcgtgtg ctttcagatg attgttaatc agcagttgca
    29161 gacctttacg cttgatggca ggcaacactg aaggcacaac ttcatcaatt aaatcctgca
    29221 cggagaacag caccgtctca cttttccagc tatcgtccgc aagcatgttc gctaactgta
    29281 tttcatcgac cagccgcacc agcacatctg cctgattcgc cagttgtttg ctttccgggg
    29341 cgttgagttt agccgcgctc tccgccaggg actgtgcagg ttctttcagc gcatcgccaa
    29401 tgtttttcat aaaggtcatc cggccctgct ggtttttctc atacagacgc tgcgcctgct
    29461 tgagtttctt gtttaccagc acttcgcgat cctgatcgcg aatgatgaaa atttgtgtgc
    29521 gcggcgcgac ctggctgcgg aacatgcgga tctcatacag ctcgttattg atcgtcgcct
    29581 gaataatccc ctgatgctgt tccgccatgg tggtgatgtt ttgcagattc aaatgcggca
    29641 gcaaatgatc ggcaattttg ttacttatga cagtgcggtt cgattcctga tcgtgaacca
    29701 gcaggccgag cggcagcagt gagactatct cttcattgat tgcccgtaaa atgcgcaatt
    29761 cgttattgac cgccgtgttg ggtagacttt ctgtactacg actggagaaa tggcggaatg
    29821 tggtatagcc aaataacgcc agcgccagca aaccgatgtt cagcagcagt ggcagcagaa
    29881 tgttttgcaa cgtatccagc aataaggtgc cataaggaac ctgccagacc aggcgcatat
    29941 cggtagagtt gagtgccgag gcgatttcaa tcttcgtact gttaaagtgg atactgacgc
    30001 tatccgtccc ttctttctca ttatcattat cattgtttcc cgttgccgtc gcgtcttgct
    30061 caaggcggaa actgtccagc ggcatacccg gtgggatcaa atcattaatc ggcagatcaa
    30121 aagccacgac cgttgccaga tgtcccggct ggttgaacgt ggtacgcaag gtaaagtaat
    30181 gaccgttctg ccaggccagg cggcgcatgt tagaaaaact ttcgcgttca tcgagggcgt
    30241 tggcctgttg caacatttct gcacgacgtg aatcaacaat gtcactgacg gtcgattctt
    30301 taaatccgga ggtgagatct ttgaggggca gggttgagat cagcaccaga ctgttatcct
    30361 gaccattcag gtaatacatt gaccacggta cattttctgc gccccacaat gtgtccagat
    30421 aggtggacat ccgttgagtc atctcaagcg ttgagttgtc gtgagagcca aagattaacg
    30481 cttccgtttt gcggcgcggt ttttccagat agtagacatc ctgtttcagg cgcgtctctt
    30541 gtaaaccttc gccggaggag ggggaggtcg tcgcggcaat attgtcgtag atctgccagg
    30601 tcacgtaacg ccaggtatcg acgcgctttt ggatagcgtg ggtaatgtcg acaatctggt
    30661 aacttttatc tttcagccag gcgttaacgg cgctttgtac cattacaccc atcgtcacca
    30721 gtaacacaat gatcaacagt aaaaagaagc gggtaatgct ccccggtagg agtgaaaagc
    30781 gggtcgtggc cgttgtctct ttctgacgca ttcgtgttta tgacctgtta aaacttcgcg
    30841 aagtgatagg gcagtataaa gggtacagtg tgaaattcca gactcttacc tgattgatga
    30901 ttctctattc tcccgtgata tttatcatcc tgcattgggg gaaatagatg tacaagcgcc
    30961 attttgctgt acaaataacc tacaaaaagc ccaacaaaaa taattgcaaa taaacaaaga
    31021 ttgctggaaa ttatgctgat gttaattatt tgtgaaatag ttaacaagcg ttatagtttt
    31081 tctgtggtag cacagaataa tgaaaagtgt gtaaagaagg gtaaaaaaaa ccggatgcga
    31141 ggcatccggt tgaaataggg gtaaacagac attcagaaat gaatgacggt aataaataaa
    31201 gttaatgatg atagcgggag ttattctagt tgcgagtgaa ggttttgttt tgacattcag
    31261 tgctgtcaaa tacttaagaa taagttattg attttaacct tgaattatta ttgcttgatg
    31321 ttaggtgctt atttcgccat tccgcaataa tcttaaaaag ttcccttgca tttacatttt
    31381 gaaacatcta tagcgataaa tgaaacatct taaaagtttt agtatcatat tcgtgttgga
    31441 ttattctgca tttttgggga gaatggactt gccgactgat taatgagggt taatcagtat
    31501 gcagtggcat aaaaaagcaa ataaaggcat ataacagagg gttaataaca tgaaagttaa
    31561 agtactgtcc ctcctggtcc cagctctgct ggtagcaggt gcagcaaacg ctgctgaagt
    31621 ttacaacaaa gacggcaaca aattagatct gtacggtaaa gtagacggcc tgcactattt
    31681 ctctgacaac aaagatgtag atggcgacca gacctacatg cgtcttggct tcaaaggtga
    31741 aactcaggtt actgaccagc tgaccggtta cggccagtgg gaatatcaga ttcagggcaa
    31801 cagcgctgaa aacgaaaaca actcctggac ccgtgtggca ttcgcaggtc tgaaattcca
    31861 ggatgtaggt tctttcgact acggtcgtaa ctacggcgtt gtttacgacg taacttcctg
    31921 gaccgacgta ctgccagaat tcggtggcga cacctacggt tctgacaact tcatgcagca
    31981 gcgtggtaac ggcttcgcga cctaccgtaa cactgacttc ttcggtctgg ttgacggcct
    32041 gaactttgct gttcagtacc agggtaaaaa cggtagcgta agcggcgaag gcatgaccaa
    32101 caacggtcgt ggtgctctgc gtcagaacgg cgacggcgtt ggcggatcta tcacttatga
    32161 ttacgaaggc ttcggtatcg gtggtgcgat ctccagctcc aaacgtactg atgatcagaa
    32221 cagcccgctg tacatcggta acggcgaccg tgctgaaacc tacaccggtg gtctgaaata
    32281 cgacgctaac aacatctacc tggctgctca gtacacccag acctataacg caactcgcgt
    32341 aggttccctg ggttgggcga acaaagcaca gaactttgaa gctgttgctc agtaccagtt
    32401 cgacttcggt ctgcgtccgt ctgtagcata cctgcagtct aaaggtaaaa acctgggtac
    32461 catcgctggt cgtaactacg acgacgaaga tatcctgaaa tatgttgatg ttggtgctac
    32521 ctactacttc aacaaaaaca tgtccaccta cgttgactac aaaatcaacc tgctggacga
    32581 caaccagttc actcgtgacg ctggcatcaa cactgataac atcgtagctc tgggtctggt
    32641 ttaccagttc taatctcgat tgatatcgaa caaagggcct gcgggccctt ttttcattgt
    32701 tttcagcgta caaacacagt tttttggtgt actcttgcga ccgttcgcat gaggataatc
    32761 acgtatggaa ataagcttta cccgcgtggc actgctggct gccgcgctct tttttgttgg
    32821 ttgcgatcaa aaaccacaac ccgccaaaac ccacgctact gaagttaccg ttcttgaagg
    32881 caaaaccatg ggtaccttct ggcgtgccag catcccgggc attgatgcca aacgcagtgc
    32941 cgaacttaaa gaaaagattc agacccagct ggacgccgac gatcagctgc tttcgaccta
    33001 taaaaaagat tccgcgctga tgcgctttaa cgattcgcaa agtttatcgc cgtggccggt
    33061 aagtgaagcg atggccgata tcgtcaccac ctcgctgcgc attggcgcga agaccgatgg
    33121 cgcgatggat ataaccgtcg ggccgctggt gaatctgtgg ggctttggcc cggaacaaca
    33181 gccggttcaa attccgagcc aggaacagat cgatgcgatg aaagccaaaa ccggcttaca
    33241 gcacctgacg gtcattaatc agtcgcatca gcaatatctg caaaaagacc tgccggattt
    33301 aaatgtcgat ctctctaccg tcggcgaagg ttatgcggcg gatcacttgg cacgcttgat
    33361 ggagcaggaa gggatttccc gctatctggt gtcggtgggc ggcgcgctga acagccgtgg
    33421 tatgaacggt gaaggccagc cgtggcgggt agcgattcaa aaaccaaccg ataaagaaaa
    33481 cgcggttcag gctgtggtgg atatcaacgg tcatgggatt agcacctctg gcagctaccg
    33541 taactattac gaactggacg gcaaacgtct ttcccatgtt atcgatccgc aaaccgggcg
    33601 tcccatcgaa cacaatctgg tatccgtgac ggtgattgct ccgacggcgc tggaagccga
    33661 tgcctgggat actggcttga tggtactcgg gccggagaaa gccaaagagg ttgttcgccg
    33721 ggaagggctg gcggtttata tgatcaccaa agaaggcgat agctttaaaa cctggatgtc
    33781 accacagttt aaaagcttcc ttgtcagcga aaaaaattaa agcgcaagat tgttggtttt
    33841 tgcgtgatgg tgaccgggca gactaaaggc tatccttaac cagggagctg attatgaaaa
    33901 aagccacatg cttaactgac gatcaacgct ggcaatctgt cttagcccgc gacccgaatg
    33961 ccgacggcga attcgttttc gccgtgcgta ccacaggcat cttttgccgt ccgtcttgcc
    34021 gcgccagaca tgctttgcgg gaaaacgtct ccttctacgc aaatgccagc gaggcactcg
    34081 ccgctggctt tcgcccctgc aaacgttgtc agccagaaaa agccaatgcc cagcaacatc
    34141 ggttggataa aatcacccac gcgtgtcgac tgctggaaca ggaaacgcct gtaacgttgg
    34201 aagccttagc cgaccaggtg gcgatgagtc catttcatct acatcggttg tttaaagcga
    34261 ctaccggaat gacgcctaaa gcctggcaac aggcctggcg cgctcgccgt ttgcgcgaat
    34321 cgctggcgaa aggggagagc gtgacgacgt ctattcttaa cgccggattc cccgacagca
    34381 gcagttacta tcgcaaagct gacgaaacgc tgggcatgac ggctaaacaa ttccgtcacg
    34441 gtggcgaaaa tctggcggtg cgttacgcgc tggctgattg tgagctgggt cgttgcctgg
    34501 tggcagaaag cgagcggggg atttgcgcga tattgctggg cgatgatgac gcgacactaa
    34561 tcagcgagtt gcagcagatg tttcccgctg ccgacaacgc gcctgccgat ctgatgtttc
    34621 agcaacatgt gcgtgaagtg atcgccagcc tcaatcaacg cgatacgccg ctgacgttac
    34681 cgctggacat tcgcggcact gcttttcagc aacaagtctg gcaggcactg cgcacgatac
    34741 cttgcggtga aaccgtcagt tatcagcaac tggctaacgc catcggcaaa ccgaaagcgg
    34801 tacgggccgt tgccagcgcc tgtgccgcca acaagctggc tatcataata ccctgtcatc
    34861 gggtggtccg tggtgatggc acactttccg gttaccgctg gggcgtgtcg cgtaaagcgc
    34921 aactgctgcg ccgcgaagct gaaaatgagg agaggtaatg ttggatctgt ttgccgatgc
    34981 tgaaccgtgg caagagccac tggcggctgg agcggtaatt ttgcaccgtt ttgcttttaa
    35041 cgctgcggag caactaatcc gcgatattaa tgacgttgcc agccagtcgc cgtttcgcca
    35101 gatggtcacc cccgggggat ataccatgtc ggtggcgatg accaactgtg ggcatctggg
    35161 ctggacgagc catcggcaag gttatctcta ttcgcccatt gatccgcaaa caaataaacc
    35221 gtggcccgcc atgccacaga gttttcataa tttatgtcaa cgtgcggcta cggcggcggg
    35281 ctatccagat ttccagccag atgcttgtct tatcaaccgc tacgctcctg gcgcgaaact
    35341 gtcgctgcat caggataaag acgaaccgga tctgcgcgcg ccaattgttt ctgtttctct
    35401 gggcttaccc gcgatttttc aatttggtgg cctgaaacgt aatgatccgc tcaaacgttt
    35461 gttgttggaa catggcgatg tggtggtatg gggcggtgaa tcgcggctgt tttatcacgg
    35521 tattcaaccg ttgaaagcgg ggtttcatcc actcaccacc gactgccgct acaacctgac
    35581 attccgtcag gcaggtaaaa aagaataaaa ataagaatta ttattgctgt gcgcgcgaag
    35641 atgtttaaac tgcgggctgt aattcattgt ccgggttttc tgcatggaac ttcttgtact
    35701 tgtctggcgg cagtatcgct ggccatttat cagtgtgatg gcgctaagcc tcgccagtgc
    35761 ggcattaggc attggcttaa ttgcttttat caatcagcgc cttatcgaaa cggcggatac
    35821 cagtctgctg gtgttgccgg agtttcttgg attattactg ctgttgatgg cagtcactct
    35881 cggatcgcaa ctggcgctca ccactttggg gcatcacttc gtttaccgac tgcgtagtga
    35941 atttatcaag cggattctgg atacccacgt cgagcgcatt gaacaactcg gtagcgcctc
    36001 gttgctggcg ggattaacca gcgatgtgcg caatatcacc attgcttttg tgcgtcttcc
    36061 ggaactggtg caggggatca ttctcactat cggttccgcg gcgtatctgt ggatgctgtc
    36121 gggcaaaatg ttgctggtaa cggctatctg gatggcgatc accatctggg gtggttttgt
    36181 actggtggcg cgggtgtaca aacatatggc gaccctgcgt gaaaccgaag acaagctgta
    36241 cacggatttt caaaccgtac tcgaagggcg caaagagctg actctgaacc gggaacgcgc
    36301 cgagtatgtg tttaacaacc tctacatccc cgatgcgcaa gagtatcgcc atcatattat
    36361 tcgcgcagac accttccatc ttagcgccgt gaactggtca aacatcatga tgctgggcgc
    36421 tatcggcctg gtgttctgga tggcgaacag tctcggttgg gctgatacca acgttgctgc
    36481 gacctattcg ttgacgctgt tattcctgcg tacgccgctg ctttcggcgg ttggcgcatt
    36541 gccgacgctg ctgacggcgc aggtggcgtt taacaagctg aacaaattcg cgctcgcgcc
    36601 tttcaaagct gagtttccgc gcccgcaggc ttttcccaac tggcaaacgc tggagctgcg
    36661 taacgtgacg tttagctatc aggataacgc gttttccgtt gggccgatta acctcaccat
    36721 taaacgtggc gagctgctgt ttctgattgg tggcaacggt agtggaaaat cgacgctggc
    36781 gatgttgctg acgggcttgt accagccaca aagcggcgaa atcttgctgg atggcaaacc
    36841 tgtcagtggc gaacaaccgg aagattatcg caaactgttt tcggcagtgt ttaccgatgt
    36901 ctggctgttt gatcaactgc tggggccgga gggtaaaccc gctaacccgc aactggttga
    36961 gaagtggctg gcgcagctga aaatggctca taagcttgag ttaagcaacg ggcgtattgt
    37021 taacctgaag ttatcaaaag ggcagaaaaa acgcgtggcg ctgttgctgg cgctggcaga
    37081 agaacgcgat attatcctgc tggatgaatg ggcggcggat caggatccac acttccgtcg
    37141 tgagttttat caggtgttgc tgccgctgat gcaggagatg ggtaaaacta ttttcgctat
    37201 cagtcatgat gatcattact ttatccacgc cgaccgcctg ctggaaatgc gcaatgggca
    37261 acttagcgag ctgacgggcg aagagcgcga tgccgcttcg cgtgatgccg ttgcccggac
    37321 ggcataatat tacgacgctg acctgg
//
LOCUS       DAJOWB010000034        37252 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJOWB010000034 DAJOWB010000000
VERSION     DAJOWB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.034"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            292..1179
                     /gene="rhtA"
                     /locus_tag="OHO85_001899"
     CDS             292..1179
                     /gene="rhtA"
                     /locus_tag="OHO85_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCQ2671275.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            1478..1981
                     /gene="dps"
                     /locus_tag="OHO85_001900"
                     /gene_synonym="pexB"
     CDS             1478..1981
                     /gene="dps"
                     /locus_tag="OHO85_001900"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCQ2671276.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            2385..3131
                     /gene="glnH"
                     /locus_tag="OHO85_001901"
     CDS             2385..3131
                     /gene="glnH"
                     /locus_tag="OHO85_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCQ2671277.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            3270..3929
                     /gene="glnP"
                     /locus_tag="OHO85_001902"
     CDS             3270..3929
                     /gene="glnP"
                     /locus_tag="OHO85_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCQ2671278.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            3926..4648
                     /gene="glnQ"
                     /locus_tag="OHO85_001903"
     CDS             3926..4648
                     /gene="glnQ"
                     /locus_tag="OHO85_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCQ2671279.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            4909..7134
                     /gene="ybiO"
                     /locus_tag="OHO85_001904"
     CDS             4909..7134
                     /gene="ybiO"
                     /locus_tag="OHO85_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCQ2671280.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGASVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(7131..8057)
                     /gene="rlmF"
                     /locus_tag="OHO85_001905"
     CDS             complement(7131..8057)
                     /gene="rlmF"
                     /locus_tag="OHO85_001905"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCQ2671281.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISSNPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVTFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            8333..8593
                     /locus_tag="OHO85_001906"
     CDS             8333..8593
                     /locus_tag="OHO85_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCQ2671282.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            8858..11140
                     /locus_tag="OHO85_001907"
     CDS             8858..11140
                     /locus_tag="OHO85_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HCQ2671283.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQNNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLMNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            11182..11859
                     /gene="ybiX"
                     /locus_tag="OHO85_001908"
     CDS             11182..11859
                     /gene="ybiX"
                     /locus_tag="OHO85_001908"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCQ2671284.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            11933..12199
                     /gene="ybiI"
                     /locus_tag="OHO85_001909"
     CDS             11933..12199
                     /gene="ybiI"
                     /locus_tag="OHO85_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCQ2671285.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            12464..12724
                     /locus_tag="OHO85_001910"
     CDS             12464..12724
                     /locus_tag="OHO85_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCQ2671286.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(12953..14038)
                     /gene="hcxB"
                     /locus_tag="OHO85_001911"
     CDS             complement(12953..14038)
                     /gene="hcxB"
                     /locus_tag="OHO85_001911"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCQ2671287.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(14179..15141)
                     /gene="ybiB"
                     /locus_tag="OHO85_001912"
     CDS             complement(14179..15141)
                     /gene="ybiB"
                     /locus_tag="OHO85_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCQ2671288.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            complement(15169..17319)
                     /gene="dinG"
                     /locus_tag="OHO85_001913"
     CDS             complement(15169..17319)
                     /gene="dinG"
                     /locus_tag="OHO85_001913"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCQ2671289.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVTKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            17439..17921
                     /gene="ybiA"
                     /locus_tag="OHO85_001914"
     CDS             17439..17921
                     /gene="ybiA"
                     /locus_tag="OHO85_001914"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCQ2671290.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            complement(18153..19517)
                     /gene="rhlE"
                     /locus_tag="OHO85_001915"
     CDS             complement(18153..19517)
                     /gene="rhlE"
                     /locus_tag="OHO85_001915"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCQ2671291.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            19746..20417
                     /gene="cecR"
                     /locus_tag="OHO85_001916"
     CDS             19746..20417
                     /gene="cecR"
                     /locus_tag="OHO85_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCQ2671292.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            20420..21415
                     /gene="hlyD"
                     /locus_tag="OHO85_001917"
     CDS             20420..21415
                     /gene="hlyD"
                     /locus_tag="OHO85_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCQ2671293.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLILA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            21408..23144
                     /locus_tag="OHO85_001918"
     CDS             21408..23144
                     /locus_tag="OHO85_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2671294.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVDGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            23137..24270
                     /locus_tag="OHO85_001919"
     CDS             23137..24270
                     /locus_tag="OHO85_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2671295.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            24281..25387
                     /locus_tag="OHO85_001920"
     CDS             24281..25387
                     /locus_tag="OHO85_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2671296.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(25349..25759)
                     /locus_tag="OHO85_001921"
     CDS             complement(25349..25759)
                     /locus_tag="OHO85_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCQ2671297.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            25892..26653
                     /locus_tag="OHO85_001922"
     CDS             25892..26653
                     /locus_tag="OHO85_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCQ2671298.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            26650..27891
                     /gene="clsB"
                     /locus_tag="OHO85_001923"
     CDS             26650..27891
                     /gene="clsB"
                     /locus_tag="OHO85_001923"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCQ2671299.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            27891..28847
                     /locus_tag="OHO85_001924"
     CDS             27891..28847
                     /locus_tag="OHO85_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCQ2671300.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(28883..29596)
                     /locus_tag="OHO85_001925"
     CDS             complement(28883..29596)
                     /locus_tag="OHO85_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCQ2671301.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVTTAGLAWVAKYL
                     EITATKYDSPQMYVAIGLLLLCMYGLSKDINKINAVIAGVMYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(29801..30505)
                     /locus_tag="OHO85_001926"
     CDS             complement(29801..30505)
                     /locus_tag="OHO85_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCQ2671302.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(30642..31094)
                     /gene="moaE"
                     /locus_tag="OHO85_001927"
     CDS             complement(30642..31094)
                     /gene="moaE"
                     /locus_tag="OHO85_001927"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCQ2671303.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(31096..31341)
                     /gene="moaD"
                     /locus_tag="OHO85_001928"
     CDS             complement(31096..31341)
                     /gene="moaD"
                     /locus_tag="OHO85_001928"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCQ2671304.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(31334..31819)
                     /gene="moaC"
                     /locus_tag="OHO85_001929"
     CDS             complement(31334..31819)
                     /gene="moaC"
                     /locus_tag="OHO85_001929"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCQ2671305.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(31822..32334)
                     /gene="moaB"
                     /locus_tag="OHO85_001930"
     CDS             complement(31822..32334)
                     /gene="moaB"
                     /locus_tag="OHO85_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCQ2671306.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(32356..33345)
                     /gene="moaA"
                     /locus_tag="OHO85_001931"
     CDS             complement(32356..33345)
                     /gene="moaA"
                     /locus_tag="OHO85_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCQ2671307.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(33333..33477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            33742..34650
                     /gene="yvcK"
                     /locus_tag="OHO85_001932"
     CDS             33742..34650
                     /gene="yvcK"
                     /locus_tag="OHO85_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCQ2671308.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(34842..36863)
                     /gene="uvrB"
                     /locus_tag="OHO85_001933"
     CDS             complement(34842..36863)
                     /gene="uvrB"
                     /locus_tag="OHO85_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCQ2671309.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         8848 a         9853 c         9564 g         8987 t
ORIGIN      
        1 aactcttcgc gatttgtgat gtctaacggg ccatttcatg taacagaacg tttccataca
       61 ccgctatcca tctaaattta aatcactttt tcagagaact gcgtaagtat tacgcatgtt
      121 ttccctgtca ttcatccaga ttattcctaa tcaccagact aatgattcca tcaatcctgg
      181 cgcattttag tcaaaacggg ggaaaatttt ttcaacaaat gctcaaccag cattgggtat
      241 atccagtaca ctccacgctt tacttaagtc tagatatttg tgggagaaag gatgcctggt
      301 tcattgcgta aaatgccggt ctggttacca atagtcatat tgctcgttgc catggcgtct
      361 attcagggtg gagcctcgtt agctaagtca ctttttcctc tggtgggcgc accgggtgtc
      421 actgcgctgc gtctggcatt aggaacgctg atcctcatcg cgttctttaa gccatggcga
      481 ctgcgctttg ccaaagagca acggttaccg ctgttgtttt acggcgtttc gctgggtggg
      541 atgaattatc ttttttatct ttctattcag acagtaccgc tgggtattgc ggtggcgcta
      601 gagttcaccg gaccactggc ggtggcgctg ttctcttctc gtcgcccggt agatttcgtc
      661 tgggttgtgc tggcggttct tggtctgtgg ttcctgctac cgctggggca agacgtttcc
      721 catgtcgatt taaccggctg tgcgctggct ctgggggccg gggcttgttg ggctatttac
      781 attttaagtg ggcaacgcgc aggagcggaa catggccctg cgacggtggc aattggttcg
      841 ttgattgcag cgttaatttt cgtgccaatt ggagcgcttc aggctggtga agcactctgg
      901 cactggtcgg ttattccatt gggtctggcg gtcgctattc tctcgaccgc tctgccttat
      961 tcgctggaaa tgattgccct cacccgtttg ccaacacgga catttggtac gctgatgagc
     1021 atggaaccgg cgctggctgc cgtttccggg atgattttcc tcggagaaac actgacaccc
     1081 atacagctgc tggcgctcgg cgcaattatc gccgcttcaa tgggttctac gctgacagta
     1141 cgcaaagaga gcaaaataaa agaattagac attaattaaa tttacatttc tgcatggtta
     1201 tgcataacca tgcagaattt ctcgctactt ttcctctaca ccgtctttat atatcgaatt
     1261 atgcaaaagc atatttattc cgaaaattcc tggcgagcag ataaataaga attgttctta
     1321 tcaatatatc taactcattg aatctttatt agttttgttt ttcacgcttg ttaccactat
     1381 tagtgtgata ggaacagcca gaatagcgga acacatagcc ggtgctatac ttagtctcgt
     1441 taattactgg gacataacat caagaggata tgaaattatg agtaccgcta aattagttaa
     1501 atcaaaagcg accaatctgc tttatacccg caacgatgtc tccgacagcg agaaaaaagc
     1561 aacagtagag ttgctgaatc gccaggttat ccagtttatt gatctttctt tgattaccaa
     1621 acaagcgcac tggaacatgc gcggcgctaa cttcattgcc gtacatgaaa tgctggatgg
     1681 cttccgcacc gcactgatcg atcatctgga taccatggca gaacgtgcag tgcagctggg
     1741 cggtgtagct ctggggacca ctcaagttat caacagcaaa accccgctga aaagttaccc
     1801 gctggacatc cacaacgttc aggatcacct gaaagaactg gctgaccgtt acgcaatcgt
     1861 cgctaatgac gtacgcaaag cgattggcga agcgaaagat gacgacaccg cagatatcct
     1921 gaccgccgcg tctcgcgacc tggataaatt cctgtggttt atcgagtcta acatcgaata
     1981 aatccatcgc tgatggtgca gaactttagt acccgataaa agcggcttcc tgacaggagg
     2041 ccgttttgtt ttgcagccca cctcaacgca cttatttagt gcatccatct gctatctcca
     2101 gctgattaag taaatttttt gtatccacat catcacacaa tcgttacata aagattgttt
     2161 tttcatcagg ttttacgcta aataatcact gtgttgagtg cacaatttta gcgcaccaga
     2221 ttggtgcccc agaatggtgc atcttcaggg tattgcccta taaatcgtgc atcacgtttt
     2281 tgccgcatct cgaaaaatca atgagttgca aaactggcac gattttttca tatatgtgaa
     2341 tgtcacgcag gggatcgtcc cgtggataga aaaaaggaaa tgctatgaag tctgtattaa
     2401 aagtttcact ggctgcactg accctggctt ttgcggtttc ttctcatgcc gcggataaaa
     2461 aattagttgt cgcgacggat accgccttcg ttccgtttga atttaaacag ggcgataaat
     2521 atgtgggctt tgacgttgat ctgtgggctg ccatcgctaa agagctgaag ctggattacg
     2581 aactgaagcc gatggatttc agtgggatca ttccggcact gcaaaccaaa aacgtcgatc
     2641 tggcgctggc gggcattacc atcaccgacg agcgtaaaaa agcgatcgat ttctctgacg
     2701 gctactacaa aagcggcctg ttagtgatgg tgaaagctaa caataacgat gtgaaaagcg
     2761 tgaaagatct cgacgggaaa gtggttgctg tgaagagcgg tactggctcc gttgattacg
     2821 cgaaagcaaa catcaaaact aaagatctgc gtcagttccc gaacatcgat aacgcctata
     2881 tggaactggg caccaaccgc gcagacgccg ttctgcacga tacgccaaac attctgtact
     2941 tcatcaaaac cgccggtaac ggtcagttca aagcggtagg tgactctctg gaagcgcagc
     3001 aatacggtat tgcgttcccg aaaggtagcg acgagctgcg tgacaaagtc aacggcgcgt
     3061 tgaaaaccct gcgcgagaac ggaacttaca acgaaatcta caaaaaatgg ttcggtactg
     3121 aaccgaaata ataacgctac acctgtaaaa cgcactggca gttccctctc ccctatgggg
     3181 agaggattag ggtgaggggc gcaaacccgc tccggggcca ttaattaccc tgaatttgat
     3241 tatttacacc acggtaacag gaacaacata tgcagtttga ctggagtgcc atctggcctg
     3301 ccattccgct tctgattgaa ggtgccaaaa tgaccctgtg gatttcggtc ctcggtctgg
     3361 caggcggtct ggtaatcgga ttgctggcag gttttgcacg caccttcggt ggttggatag
     3421 ccaaccacgt cgcgctggtc tttattgaag tgatccgcgg cacacctatc gtcgtccagg
     3481 tgatgtttat ctatttcgcc ctgccgatgg cgtttaacga cttacgcatc gacccattta
     3541 ctgcggcggt ggtcaccatc atgatcaact ccggcgcgta cattgcggaa atcacgcgtg
     3601 gtgcggtgct gtctattcac aaaggttttc gtgaagcagg actggcgctc ggtctttcac
     3661 gttgggaaac cattcgctac gtcattttac cgctggcact gcgtcgtatg ctgccgccgc
     3721 tgggtaacca gtggatcatc agcattaaag acacctcgct gtttattgtt atcggcgtgg
     3781 cggaactgac ccgtcagggg caagaaatta ttgccggtaa cttccgcgcc cttgagatct
     3841 ggagcgccgt ggcggtgttc tatctgatta ttaccctggt gctgagcttt attctgcgtc
     3901 gtctggaaag aaggatgaaa atcctgtgat tgaatttaaa aacgtctcca agcattttgg
     3961 tccaacccag gtgctgcaca atatcgattt gaacattgct cagggcgaag tcgtggtgat
     4021 tatcgggccg tccggttccg gtaaatcgac cctgctgcgc tgcatcaaca aactggaaga
     4081 aatcacctcc ggcgatctga ttgtcgatgg cctgaaggtt aacgatccga aagttgacga
     4141 gcgcctgatt cgccaggaag cgggtatggt gttccagcag ttttacctct tcccgcatct
     4201 gacggcgctg gaaaacgtca tgtttggtcc gctacgcgtg cgtggcgcga acaaagaaga
     4261 ggcggaaaaa ctggcacgtg agctgctggc gaaagtcggt ctggcagaac gtgcacatca
     4321 ctacccttcc gaactttctg gtggtcaaca gcagcgtgtg gcgattgccc gcgcgctggc
     4381 ggtgaagccg aaaatgatgc tgtttgatga accgacttcc gctcttgacc cggaactgcg
     4441 ccatgaagtg ctgaaggtta tgcaggatct ggctgaagaa gggatgacga tggtgatcgt
     4501 gacccacgaa atcggttttg ccgagaaagt agcttcgcgg ctgatcttta tcgacaaagg
     4561 ccggattgcg gaagatggca atccgcaggt gttgatcaag aacccgccga gccagcgctt
     4621 gcaggaattt ttgcagcacg tctcttaata agacacattg cctgatgcgc tacgcttatc
     4681 aggcctacag gatatctggc aacttattaa aattgcatga acttgtagga cggataaggc
     4741 gttcacgccg catccggcaa aaaagcccgc acgttgtcag caacctgctt aatatccctt
     4801 cctccctttc acccgaaagg gaggcacacc agattcctct catttaaaat cgcccctcct
     4861 ccagcatcta tacttatctt tttgctctat tttctcactg gaggagtcat gcggtggatc
     4921 ctgttcatcc tcttctgcct gctgggcgca cctgcccacg cggtatccat acccggcgtt
     4981 acaaccacaa cgacaacgga ctcaacgact gaaccggccc cggaaccgga tatcgaacaa
     5041 aaaaaagcgg cctatggcgc actggcggat gtgctggata atgacacctc gcgtaaagag
     5101 ttgatcgacc agttgcgcac cgttgccgct acgccccctg ctgaaccggt accaaaaata
     5161 gtgccgccga cgctggtcga agagcaaacc gtgctgcaaa aggtcaccga agtcagccgc
     5221 cattatggtg aagccctttc cgcccgcttc gggcaacttt atcgcaatat caccggctcc
     5281 ccgcataagc cgtttaatcc acaaaccttc agcaatgcgc tgacccattt ttcaatgtta
     5341 gcggtattag tgtttggttt ttactggctg attcgcctgt gcgcactgcc gctgtatcgc
     5401 aaaatgggtc agtgggcgcg gcaaaaaaat cgtgagcgca gcaactggtt gcagcttccg
     5461 gcgatgatta tcggggcgtt tattatcgac ctgctgttac tggccctgac attatttgtc
     5521 ggccaggtat taagcgacaa cctgaatgca ggcagtcgca ccatcgcttt ccaacaaagt
     5581 ttgtttctca acgcctttgc cctcattgaa tttttcaaag ccgtactacg cctgattttt
     5641 tgcccaaacg tggcggagct gcgcccgttc acgattcagg acgagagcgc ccgttactgg
     5701 agtcgtcgcc tgagctggtt aagcagtttg ataggttatg gcctgattgt ggccgtgccg
     5761 attatctcta atcaggtgaa tgtacagata ggtgcgctgg cgaacgtcat cattatgctg
     5821 tgcatgaccg tctgggcgtt gtacctgatc tttcgtaata aaaaagagat tacccagcat
     5881 ttgctcaact tcgcggagca ttcgctggcc tttttcagcc tgtttatccg cgcctttgcg
     5941 ctggtgtggc actggctggc aagcgcctat tttatcgtgc tgtttttctt ttcgttgttc
     6001 gatccgggca acagcctgaa atttatgatg ggtgcatcgg tgcgcagcct ggcgattatt
     6061 ggtatcgcag cgtttgtttc cggtatgttt tcccgctggc tggcgaaaac catcactctc
     6121 tcgccacata ctcagcgtaa ctatccggag ctgcaaaaac ggttgaatgg ctggctgtcg
     6181 gcggcgctga aaacggcgcg tattctgaca gtctgcgtgg cggtaatgtt gctgttgagc
     6241 gcatggggat tgttcgattt ctggaactgg ctgcaaaacg gcgcggggca gaaaaccgta
     6301 gatatcctga tccgtatcgc actcattctt ttcttctcgg cggttggctg gacggtgctc
     6361 gccagtttga tcgaaaaccg gctggcttcg gatattcatg gccgcccgct acccagcgcc
     6421 cgcacgcgta ccctgcttac gctgtttcgt aacgcgctgg cggtgattat cagtaccatc
     6481 accatcatga ttgtgttgtc ggaaatcggc gtcaatatcg cgccattgct ggcaggtgcc
     6541 ggggcattag gtctggctat ctcgtttggt tcgcaaacgc tggtgaaaga tattatcacc
     6601 ggggtattta ttcagtttga aaacggcatg aacactggag atttggtgac tatcgggccg
     6661 ttgaccggca cagtggaacg gatgtcgatt cgctccgtgg gcgtacgcca ggataccggg
     6721 gcgtatcaca tcattccgtg gtcttcgata accacctttg ctaactttgt ccgcggcatt
     6781 ggttcggtgg tggcaaatta tgatgttgat cgccatgaag atgctgataa agccaatcag
     6841 gcactgaaag atgcggtagc ggaattaatg gaaaacgaag aaattcgcgg gctgattatt
     6901 ggtgaaccga attttgccgg gattgtcggc ttaagcaata ccgcgtttac actgcgtgtt
     6961 tcgttcacca cgctgccact caaacagtgg acggtacgct ttgccctcga cagccaggtg
     7021 aaaaaacatt tcgacctggc gggcgttcgc gcgccagtgc agacttatca ggtgctgcct
     7081 gctccgggcg cgaccccggc tgaaccgtta ccgccggggg aaccaacgct ttaacgctgg
     7141 cgattgacaa aacggcggcg ctgctcgtcg ttcataaagg tccaggcaat aaagcgactc
     7201 tgcttttgcc cctgggccat ctctttttta accaccttca ccgcgcccac gtccgtcagg
     7261 gcacgataca acggcggtaa gttttcacca cgagatacca gtgatgtaaa ccacatcacc
     7321 tgcttcgcga agcctttgct ctcttcaatc atctttttga taaaggtgac ttcaccgcct
     7381 tcacaccaca actcctgttg ctggccgcca aagttcagtg catcgtcttt gttcagcccc
     7441 aggttacggc gtttacgctc actacctgcc cgtgccgcag cagcggaatc gtggaatggc
     7501 gggttacaca aggtcgcgtc gtattgctcg tttttatgga tgatgccgtt aaaaatcgcc
     7561 ccactctctt tttgccgacg cagacgaatg gcgcggttaa gccccggatt agaactgata
     7621 atcgcctgcg cactgcttaa cgcctggctg ctggtttcac taccggtaaa acgccagcca
     7681 tattcatgta cgccaatcag cggataaata cagttcgcac caacgccgat atccagaatg
     7741 ctggcattcg ctggaattgt tccgcttgct tctgccagta aatcggcaag gtgatgaata
     7801 taatccgccc ggcccggtac tggtgggcag agaaaaccgt cggggatatc ccagttcgct
     7861 acggcgtaaa aatgggccag caacgcctta ttgagcgcct tcaccgccag cggattggca
     7921 aagtctacgc tttgctcccc ggcgggtgta agcgtgagga attgcctgag ttcaggattg
     7981 acctgacaaa gcgtggcgag atcgtagcgg ctgtgatgac ggttgcgcgg atgcaacccc
     8041 ggtttctggg cggacatagc attctccttt aagctgcggc gtaagatacc cgcataacgc
     8101 gacagggtaa atatctggct gcgtttatca ttccccaaaa ccttaacatg gctatggttt
     8161 atggcaattc atcataaaaa tcagcattat gcatgtcatt gatttgtctt attttgtaaa
     8221 aatatatcgt tcaaaaaata agcacatctc tgtcaatttt ttgcgtcatt ttcatcgccc
     8281 aggcgaccta cacttatcag gcactacccg ttgtatttat gaggatgtca ttatgaaaaa
     8341 gtgcctcaca ctactgattg ccaccgtcct gagcggaatc tctctcacgg cttatgccgc
     8401 gcaaccgatg agtaacctgg acagcggtca gctacggccc gccggtaccg tttcggcaac
     8461 tggcgcatca aacctaagcg atctggagga taaactggca gaaaaagcgc gcgagcaagg
     8521 cgcgaaaggt tatgtcatta attccgcagg cggaaatgac cagatgttcg gtactgcaac
     8581 catctacaaa taaccccacc aacgtaactg cattcgcctc tggatgcagt tttcgcctgt
     8641 tttgtaatca aaaaacatca aatatgaacg caatgtaaat aaatgtattt ctttttcgcg
     8701 caatgggtga tagaaaatcg cgccaagtga taatgcttat aaaaattatt atcacttgca
     8761 cgaacactat cacgagatta acagtggcat cgcatccgca gagaggcttt ctcgtggcag
     8821 tgaaaatttc aacatataaa aaaaagtcac ctgcaaaatg gaaaacaatc gcaatttccc
     8881 tgccagacaa tttcattcgc tcacgttctt tgccggtctt tgtattggca tcacgcctgt
     8941 ggctcaggca ctcgccgccg aagggcaaac taacgcggat gacacgctgg ttgtcgaagc
     9001 atcaacgcct tcgctttatg cgccacaaca atctgccgat ccgaaattct cgcgtccggt
     9061 agcggatact acccgcacga tgacggtgat ttctgaacaa gtgattaaag atcagggcgc
     9121 aaccaacctt accgacgcgc tcaaaaacgt ccccggcgtg ggtgcgtttt ttgcgggtga
     9181 gaacggtaac tccaccactg gcgacgccat ttatatgcgc ggtgctgata cctctaacag
     9241 tatttatatt gatggcattc gcgatatcgg cagcgtctcg cgcgacacct tcaatactga
     9301 gcaggtcgaa gtgattaaag ggccttccgg caccgactac gggcgcagcg cgccgacggg
     9361 ctcgatcaat atgatcagca agcagccgcg caatgattcc ggcattgacg cctccgccag
     9421 tattggcagc gcctggttcc gccgcggcac gctggacgtc aatcaggtca ttggtgatac
     9481 cactgcggtg cgcctgaatg tgatgggcga aaaaacgcac gatgccggac gcgacaaagt
     9541 caaaaatgag cgttacggcg tcgccccttc tatcgctttt ggccttggta cagcgaatcg
     9601 tttgtatctt aattatctgc atgtcaccca gcacaacacg ccagacggcg gcattccgac
     9661 catcggtttg ccgggttatt ctgccccatc tgcgggaaca gcggctctga atcattccgg
     9721 aaaagttgat actcataact tttacggcac ggattccgat tacgacgatt cgaccaccga
     9781 caccgccacc atgcgttttg agcacgacat caacgataac accaccattc gcaatactac
     9841 ccgttggtcg cgcgtaaagc aggattacct gatgacggcg attatgggcg gggcgtcgaa
     9901 tattactcag cccaccagcg atgtgaatag ctggacctgg tcacgcacgg cgaataccaa
     9961 agatgtgagt aataaaattc tcaccaacca gaccaacctg acctcgacgt tctataccgg
    10021 ttctatcggt catgatgtca gtaccggcgt ggaatttacc cgtgaaacgc agaataacta
    10081 cggcgttaat ccggtgacgt tacccgcggt aaatatttat catcctgaca gcagcattca
    10141 tcccggcggc ctgacgcgca acggcgcaaa cgccaatggt cagacggata ccttcgcaat
    10201 ttacgccttt gatacgctgc aaatcacccg tgattttgag ctgaacggcg ggatccgtct
    10261 ggataattat catactgaat atgacagtgc caccgcctgc ggcggcagcg gacgcggtgc
    10321 catcacctgc ccaactggtg tggcaaaagg ttctccggtc accaccgtcg acaccgccaa
    10381 gtcgggcaat ctgatgaact ggaaagccgg ggcgctgtat cacctgacgg aaaacggcaa
    10441 tgtctatatt aactatgccg tttcccagca gcctccgggc ggcaacaact tcgcccttgc
    10501 gcagtctggc agcggtaaca gtgccaaccg caccgatttt aaaccgcaaa aagccaacac
    10561 cagcgagatt ggcaccaaat ggcaggttct ggataaacgt ctgttgctca ccgccgcgct
    10621 gttccgtact gatatcgaaa atgaagttga gcaaaatgat gacggaactt actcgcaata
    10681 cggtaagaaa cgcgtcgaag gctatgagat atccgtggcc gggaatatca ctcccgcgtg
    10741 gcaggtgatt ggcggctata cccagcaaaa agcaaccatc aaaaacggca aagatgttgc
    10801 ccaggatggt tcctcatcgc tgccgtatac cccggagcac gccttcacct tatggagcca
    10861 atatcaggca accgacgata tctctgttgg cgcgggcgca cgctatatcg gcagtatgca
    10921 taaaggttca gacggcgcgg tgggaacgcc agcgtttacc gaaggttact gggtcgccga
    10981 tgccaaactg gggtatcgag ttaatcgcaa tctcgacttc cagctaaacg tttacaacct
    11041 gtttgatacc gattacgtcg cctcaatcaa caagagcggc taccgttatc acccgggcga
    11101 gccaagaacc ttcttgctca cagccaatat gcatttctga ttcagatgtg gggcgcaggc
    11161 cccacttttt ggagaaattg tatgatgtac cacattcccg gcgtgttatc gccacaggac
    11221 gtcgctcgtt ttcgcgaaca actggaacaa gccgaatggg tggatggacg cgtcaccacc
    11281 ggcgcacaag gtgcgcaagt taagaacaat caacaggtcg acacccgcag cacgttatac
    11341 gccgccctgc aaaatgaggt gctgaacgcg gttaaccaac atgctttatt ctttgccgcg
    11401 gccttgccgc gtaccctttc cacgccgctg tttaatcgct atcagaacaa tgaaacctat
    11461 ggtttccatg tggatggcgc agtacgcagc catccacaaa acggctggat gcgtactgac
    11521 ctttctgcca cgctgttttt aagcgatcca caaagctacg acggcggcga actggtcgtt
    11581 aatgacacct tcggacaaca tcgggtaaaa ctcccggcag gcgatctcgt gttgtatccc
    11641 tccagcagcc tgcattgcgt gacacccgta acccgcggcg tacgagtggc atcatttatg
    11701 tggatccagt cgatgatccg cgatgataaa aagcgcgcca tgctgtttga actggacaac
    11761 aatattcagt cgctgaaaag ccgctacggc gaaagtgaag agatcctgtc gctgcttaat
    11821 ctttatcata atctgctgcg ggaatggtcg gagatctgat gcttgaactg cccgagttcg
    11881 tccctggatt agctacactt aactgtacaa gtattgatat ggggaggtcg atatggcatc
    11941 cggttgggct aacgatgacg ccgtcaacga acagatcaac agtacaattg aagatgcgat
    12001 tgcccgcgct cggggtgaaa ttccgcgcgg cgaaagcctg gatgaatgtg aagagtgcgg
    12061 tgcccccatc ccgcaggccc gtcgggaagc cattcctggc gtgcgcttat gtattcattg
    12121 tcagcaggag aaagatttac aaaaaccagc ttatacagga tataatcgca gaggttcgaa
    12181 agacagccag ttacgttaac tacaactggc gagatgcata gcgagtataa tttgtatttt
    12241 gcgtggcatt ccgtgccttt aacgctgctg tgactgccac gcggtataaa acaagttcat
    12301 aagtacaaca aataaatggt ttatcagtag gttagatatc aatcaattta tttgaacaag
    12361 gcggtcaatt ctcttcgatt ttatctctcg taaaaaaacg tgatactcat cacatcgacg
    12421 aaacaacgtc acttatacaa aaatcacctg cgagagatta attatgaaaa ctatcaatac
    12481 tgttgttgct gctatggctc tttcaactct gtcatttggc gtgttcgcgg cggaaccggt
    12541 aacggcatcc caggcacaga acatgaataa aatcggcgtg gtttctgccg atggcgcatc
    12601 caccctcgat gccctggaag cgaaactggc tgagaaagcc gcggcagccg gtgccagtgg
    12661 atacagcatc acttccgcca ccaacaacaa taaattaagc ggtactgcgg taatttataa
    12721 gtaattcgta ggacggataa ggcgtttacg ccgcatccgg caacagatgc ctgatgcgac
    12781 gctaccgcgt cttatcaggc ctacaaaatc cgaaccgttg gacggatcag gcgtttacgc
    12841 tacgtccagc acaagtattt ccctgtctgt ttgccgacag acgcatatgc tctaaccctc
    12901 attgatccta tgttaccctt gtttgcccgt ccgccactgg acgggctttt ttttagctgg
    12961 ctaactgctg acagaaagcc tgcaacgtct cttccggcat accaatctgc cgcgctgcat
    13021 cacaaatggc ctgccagctt cccgcatcca gtggaatccc ctgcttctgc cgttcgcgac
    13081 gcgtgttcac ttcccactcg cccggtagca aaatcggctt atcatcatca tgcggcgagg
    13141 ctttcaccca ctcggcaaag gcttcggtct gcgcgttaca atccggcgcg ccgaagagtt
    13201 ccgggttgat gatgatagtg gtcatgcagt taagaatggc atcgggactg gtttgtaacg
    13261 tttcctgatg cgtcgtttta ccgccggaaa gcgccccgcc aagaatttca cacatcgctg
    13321 caagggcgta gcctttatgt tcggcaaagg tcaacagcga acccaacggc gactcctgca
    13381 ttaccgccgg attggtcgtc ggcacgccgt taacgtcaat caggcaacct ggcggcacgg
    13441 ggacgccttt atgccaggcg acgcgggttt tgccaaatgc aatggcgctg gtggcgtaat
    13501 caagcaacag cgggaaatta tctttacgag ggaaaaccac acagaacgga ttggtgccaa
    13561 agcggctgtc gcgaccgtgg aacggcgcga ccattggaat accgaccacg ctaacaaagt
    13621 ggatagagac aaaccccgcc gctgcacact gctccgccca gtaaccgata cggccgatat
    13681 gatgcgagtt atgtagcgcc acggcggcaa taccgtgctg atgcgctttc tcaatcccca
    13741 gcgccatcgc ttcatgtgcc gcgacctgac caaatgcgcg atcgccatcg agcgtgaccg
    13801 ccgcccccgc ctctttaacg gttttggcat gatggttaat ttgcaggtgc ccctgactcc
    13861 aggagcgtac atagcttggg atcatgccaa taccatgtga atcatgccct gccaggtttg
    13921 ccgcgattaa atgatcggca actaatttcg cttcttgttc ctcgctaccc atctgacgaa
    13981 atacagcctg aataaaactg tgcagcgtct gagcatcaaa gcgatgacca ctttccatgg
    14041 ttaactcctg tttatgttat gtgtttgttg tatttttatg ctgcacgccg ggcgtgggaa
    14101 ggtcaataaa gatgcaggaa tttcatattg cggtggggta aaagccggat gacatggctc
    14161 atccggctga gaaaagaatt aaaatgcctg attaacgcgc gccaggccgt cgctgatagt
    14221 tgccgcttca cccgtagcca ccaggcagca agccatctgg attttcagcg attcgggaat
    14281 cggttcgctg ccagcaaggc aacgctcaat ccactgcgcc gtggtttccg gatcttttgc
    14341 ttgtggcagt aactcgctac cagcagtgtc ctgtttttca tacagcaccc gcatcccttc
    14401 acggtcaatg agattgatct gcgggcagcg ctgcggatta gcatacactt caccttcagt
    14461 accatgcatt agtagcgccc gcccgccgat atcgctaaag aacttcgcga cgcgtccaat
    14521 gtattccgga tgcgaaacgc tggaaagacg cagcgcctca ccttcggcaa atggcgtcgc
    14581 cagtttcgcc agggtatgtg cactgttacg cacgcccatc cgccagcgca tcgccagttg
    14641 tttttccagc ggcgggcaaa acgcgccgac tggcataaac accggttgat gttcgtcgag
    14701 cttcgcctgc gcctgcccgc cgtgcaacgt tggcgtaata cccatcaatt caaaaatggt
    14761 ttcagtcagc acgcgggttg gatcttcgct aaccccgtga accaccacag gaaaaccgag
    14821 tttgtgcagg agaatcgcca acaacggcgt caggttggcc tgtttacgcg cgccgttgta
    14881 actggggatg acaatcggca tcggcttgcc tgctggcggc gtcagcttga tggtgtgatt
    14941 ttgcatggct tcgtaaaagc cgagcatctc tgcttcccct tctcctttga tacgcagcgc
    15001 aatcaatacg ccgcccaact caaggtcagg gacttcacca ttgagcatat gagcatacag
    15061 accgcgcgca gtatcccggt ctaaatcgcg cgcgtggttt ttcccgcgcc cgatctcttt
    15121 aatgattttg cgatagtcca tttacgactc cttacctgac tcacatcatt aacgccgccg
    15181 acggcgtggg gatttcgttt tttctttctt tttaactata ccttcaggga cttccggttg
    15241 ctctatcgga aataccggta atgcatccag tagtcgcttg ccataatttt tggtcagcaa
    15301 gcgtttgtcg tagataacca cttcgcccca gcaaccgtgg cttcgaatca gtcgcccaac
    15361 ctgctgaatc aggttaaacg aggcgctcgg caggctttgc acctcaaacg gatagcggtt
    15421 gaggcttttc agccattccc cttcggtgat caccaccggg ctgtcgatgg gcggaaaagc
    15481 gattttgtgg atatgcacct ggctgagcag atcacctttc aaatcaagcc cttcggcaaa
    15541 tgactgtaag cccaccagca cgctgcgctc accgttggcg acgcgtttgc ggtgcagttc
    15601 aactaaacgg taacgcggct gatcgccctg aaccagcaac atcagacgta aatccgtcac
    15661 atagtcgaga aagcgctgca tcgcccgtcc gctggcaaac agtaccaaca taccgagatg
    15721 ttttttgctc tccacctgct tacggaaaaa ggccgccatt tcggcaatat gctgctcttc
    15781 gttgtcgatg gaaggctcaa cgcgcatccg gggaataaca attttgccct gttcgcagtg
    15841 gttaaagggg gaatccagcg ccacaaaacg gtcgcccgct ttctctttca gaccactcat
    15901 ctcctgcaaa cgcgaaaaac tgttcagcga acgcaaggtt gcggaggtga caataatgtg
    15961 cggaatactg cgccacagca gcctttccag ctgatcgctg acacgtattc ccacgcagtg
    16021 aaaccagagg tgtagctgcc cttcgcgctc ttcccgcgtc gcccatttgg tcaccggtgc
    16081 accggaagat tgcgccagcg aagccagccg ccagagtttg ctttgcgcct cgaacatccc
    16141 caacgcgcgg ttcatctgca aaatcaaccg atgcagacgt acaatgtcat ggctgccggt
    16201 tttctcactt aaatcgttaa gaaataactc cgccaggcca cgcagcatct cggtgagttt
    16261 tgccagccgc tggcagatct ccagcacttc atctggcagt tcgcccatcg caaaacggtg
    16321 ctctgcctcc tgcccggcag gcatgtagag attgagaatg ttgtttaacg aggcgataag
    16381 ctcatacaac tcttcacaat gcgcattcaa acgttcaggg atcgccagcg gtgggatggt
    16441 cttcgggcga aactgctcca tgcaggtagc gaccagtttc gtgaacaagt ccagctgtag
    16501 tcgataccag ggcgcggtga tttcagcgct catctccagc gcatcccggg ccacatctgg
    16561 caggtgatga ccttcgtcca gcaccagcag taaatttttc gggtcaggca ataccgcttc
    16621 gctttccatc gccgccatca ccagcgcatg gtttgccacc accacttccg cttcctgaat
    16681 ctcccgacga gcgacaaaaa acgggcattc acgatagtag taacaattgc ggttgaggca
    16741 gctggctttg tcggtactta aacgacgcca gagatcgtca tctatagcga tatcagtatg
    16801 atcgcgcaga ccatcccatt tataagtgtc gagatcgccc ttcagcttcg cacaacgttt
    16861 ttgctcttcc tgattgttcg gcgtcagttc gtcgtcaaga aacgccagca gatcctgttg
    16921 cgtgggctca gtgctggcaa gcgccgtcag attacgcggg caaacgtagc gcccacgacc
    16981 aaaagcggca gtgaatttaa gatcgggaat gatctttttc agcagcggta aatctttgct
    17041 gtaaatctga tcctgcaacg ccacgttggc ggtactcacc accagcgttt tttgctcttc
    17101 gcgggcaatg gcgatgccgg ggatcaaata ggagagcgtt ttcccaacgc cggtgggggc
    17161 ttcaatcgcc agatgccgcc cttcttctcc ggccagcgtt ttggtgacgt ccgcaatcat
    17221 ctgccgctgc ggcgcacggg gaataaagtc ggggatctgt tcctgaagcg ccttatacca
    17281 ggcggcaatt tgcgctttaa gcgcggcggt taatgccatg agaaaacctg aaatactgta
    17341 taaacagcca atattgtggc atttttcggt tttcaggggt aatggttttt ctgtttctgg
    17401 aaagcggctc gcaataacca ccaacataat gcggtttaat gcccgttcga gcacaaagga
    17461 tccaacacgt catgcaagac accatcataa atttctacag caccagcgat gactacggtg
    17521 atttttctaa ctttgccgcc tggcccatca aggttgacgg gaaaacctgg cccacctcag
    17581 aacactattt tcaggcacaa aaattccttg atgaaaaata ccgcgaagag atccgtcggg
    17641 tttcttcccc tatggtcgcc gcacgcatgg ggcgcgatcg ttctaagcct ctgcgtaaaa
    17701 actgggagtc ggtcaaagaa caggtgatgc gaaaagcact tcgcgctaaa ttcgaacagc
    17761 atgcagaact gcgtgcgctc ttgctggcaa ccgcgcccgc aaaactggtt gagcatacgg
    17821 aaaacgatgc ttactgggga gacggtggta atggtaaggg caagaatcga ctgggctacc
    17881 ttttaatgga gttgcgcgaa caattggcta tagagaagta acgtctcggt ggcttatggg
    17941 cggtaaaaca gataaatttt ttcccgtgtt ctcaaattgc tgacaagtgc ttgttgtgaa
    18001 atgccggatg cggcgtaaac gccttatccg gcctacaaaa gcatgcaaat tcaatatatt
    18061 gcagagttca cgtaggcctg ataagcgtag cgcatcagac agttttgcgt ttgttcatca
    18121 gcctgatgcc gggcatagcc cggcataaaa gattactgcg cagcggcagg tttacgcgga
    18181 cggcggcgac gttgttgttc gcctgccggt ttggcatcgc cgaggcggcg agacggtttt
    18241 tctgcaggtt tcgcgcttgc agattttgcg ccaccttccc cacggcgtgg ttgctgttga
    18301 ccgcgaccac caccttgccc acgaccgccg ccgccacgtt gctggcgacc gttctggatc
    18361 ggttcggctt tgattgacgg gtccggctca tagcccggaa tcgcaatgcg cgggatctct
    18421 tttttcagca gtttttcgat atcacgcagc agtttgtgtt catcaacaca caccagcgac
    18481 aacgcttcac cggtagcagc cgcacgaccg gtacgcccga tacggtggac ataatcttcc
    18541 ggtacgtttg gcagttcata gttgacgacg tgcggcaact cttcgatatc cagaccgcgc
    18601 gcagcgatat cggtcgccac cagtacgcga atgtcacccg atttaaaatc agccagcgca
    18661 cgggtacgcg caccttgcga tttattcccg tggatcgccg cactacggat gccatcttta
    18721 ttgagctgct cagccagatg gttagcgccg tgtttggtac gggtaaacac cagcacctgc
    18781 tgccagttcc ctttaccaat catgtgcgac agcaattcgc gtttgcgttt cttatcgaca
    18841 aagtgaacgt gctgggtcac ctgatcagac gcggtattgc ggcgtgccac ttcgatttcc
    18901 agcgggttgt gcaacagttt ttccgccagg gctttaatat cgtcagagaa ggtcgcagag
    18961 aacagcaggt tctggcgttt cgctggcagt tttgttaaca cgcgacggat atcgtggata
    19021 aagcccatgt cgagcatgcg gtcagcttca tcgaggacga ggatttcaac ctgatccagc
    19081 ttcactgcat tttgatgttc caggtccagc aaacgtcccg gtgttgccac cagaacatca
    19141 acgccgccgc gcagcttcat catttgcggg ttaatgctga caccgccaaa caccaccagc
    19201 gaacgaatgt tcaaatattt gctgtaatca cggacgtttt cgccaatctg cgcagccagt
    19261 tcacgggtcg gggtaagaat gagcgcacgt accggacgac gccctttggc gtgcggctgg
    19321 cgagtgatca ggtgttgcaa cagcggcagc gtaaagcccg ctgttttacc ggtaccggtc
    19381 tgggcgctag ccatcaggtc gcggccttcc agcaccgcag ggatcgcctg ctgctgaata
    19441 ggggtgggtt cacggtaacc ctgctcggca acggcgcgca ggatatcagg gcttaaaccc
    19501 aaagaatcga aagacataac tactccgaac cgccccgacc gttaccggtg tagttttcag
    19561 ggagatactg aaaaggaaaa tgacaaaaac cacaatgtca tgagggagcg gagtgtagca
    19621 gtttttgtga cgcagcgcat aaattatccc tgtgactggc gtagctaaaa ttcttagcca
    19681 tactggacaa ctcccatatg gagtcataat cttaatcaat catttgatta agaatggact
    19741 gtgcgatgaa taatcctgcc atgacaatca agggtgaaca ggcgaaaaaa cagctgattg
    19801 ctgccgcact ggcgcagttt ggtgaatatg gaatgaacgc caccacccgc gagatagccg
    19861 cccaggccgg gcagaatatc gctgccatca cctactactt cggttcgaaa gaagatttgt
    19921 acctcgcctg cgcccagtgg attgccgatt ttattggcga gcagttccgt ccgcatgccg
    19981 aggaagccga acgcttgttc gcccaaccac agcctgatcg ggctgccatc cgtgaactga
    20041 tccttcgcgc ctgcaggaac atgattaagc tgctcaccca ggatgacacc gtcaacctca
    20101 gcaagtttat ctcccgtgag cagctctctc ccacggcagc ctaccacctg gtgcacgaac
    20161 aggtgattag tccgctacac agccacctga cacgcctgat tgccgcctgg accggcagcg
    20221 acgccaatga cacccgcatg atccttcata cccatgcgtt gattggtgag attctggcgt
    20281 tccgtcttgg caaagaaaca attctgttac gtaccggctg gaccgcgttc gatgaagaaa
    20341 agaccgaact gatcaaccag acggtgacct gtcatatcga cctgattttg caaggattat
    20401 cgcaaaggag tttgtagtga tgaaaaaacc tgtcgtgatc ggactggcgg tagtggtact
    20461 tgccgccgtg gttgccggag gctactggtg gtatcaaagc cgccaggata acggcctgac
    20521 gctgtatggc aacgtggata ttcgtacggt gaatcttagt ttccgtgttg gggggcgcgt
    20581 tgaatcgctg gcggtggacg aaggtgatgc tatcaaagcg ggccaggtgc tgggcgaact
    20641 ggatcacaag ccgtatgaga ttgccctgat gcaggcgaaa gcgggtgttt cggtggcgca
    20701 ggcgcagtat gacctgatac ttgccgggta tcgcgatgaa gaaatcgctc aggccgccgc
    20761 agccgtaaaa caggcgcagg ccgcctatga ctatgcgcag aacttctaca accgccagca
    20821 agggttgtgg aaaagccgca ccatttcggc aaatgacctg gaaaatgccc gctcctcgcg
    20881 cgaccaggcg caggcaacgc tgaaatcagc acaggataaa ttgcgtcagt accgttccgg
    20941 taaccgtgaa caggacatcg cccaggcgaa agccagcctc gaacaggcgc aggcgcaact
    21001 ggcgcaggcg gagttgaatt tgcaggactc aacgttgata gccccgtctg atggcacgct
    21061 gttaacgcgc gcggtggagc caggcacggt cctcaatgaa ggtggcacgg tgtttaccgt
    21121 ttcactaacg cgtccggtgt gggtgcgcgc ttatgttgat gaacggaatc ttgaccaggc
    21181 ccagccgggg cgcaaagtgc tgctttatac cgatggtcgc ccggacaagc cgtatcacgg
    21241 gcagattggt ttcgtttcgc cgactgctga atttaccccg aaaaccgtcg aaacaccgga
    21301 tctgcgtacc gacctcgtct atcgcctgcg tattgtggtg accgacgccg atgatgcgtt
    21361 acgccaggga atgccggtga cggtgcaatt cggtaacgag gcaggacatg aatgatgccg
    21421 ttatcacgct gaacggcctg gaaaaacgct ttccgggcat ggacaagccc gccgtcgcgc
    21481 cgctcgattg taccattcac gccggttatg tgacggggct ggtggggccg gatggtgcag
    21541 gtaaaaccac gctaatgcgg atgctggcgg gattactgaa acccgacagc ggcaatgcca
    21601 cggtgattgg ctttgatcca atcaaaaacg acggcgcgct gcacgccgtg ctcggctata
    21661 tgccgcagaa atttggtctg tatgaagatc tcacggtgat ggagaacctc aatctgtacg
    21721 cggatttgcg cagcgtcacc ggcgaggcac gtaagcaaac ttttgctcgc ctgctggagt
    21781 ttacgtctct tgggccgttt accggacgcc tggcgggcaa gctctccggt gggatgaaac
    21841 aaaaactcgg tctggcctgt accctggtgg gcgaaccgaa agtgttgctg ctcgatgaac
    21901 ccggcgtcgg cgttgaccct atctcacggc gcgaactgtg gcagatggtg catgagctgg
    21961 caggcgaagg gatgttaatc ctctggagta cctcgtatct cgacgaagcc gagcagtgcc
    22021 gtgacgtgct gctgatgaac gaaggcgagt tgctgtatca gggggaacca aaagccctga
    22081 cacaaaccat ggccggacgc agctttctga tgaccagtcc acacgagggc aaccgcaaac
    22141 tattgcaacg cgccttgaaa ctgccacagg tcagcgacgg catgattcag gggaaatcgg
    22201 tacgtctgat cctcaaaaaa gaggccacac cagacgatat tcgccatgcc gacgggatgc
    22261 cggaaatcaa tatcaacgaa actacaccgc gctttgaaga tgcgtttatt gatttgctgg
    22321 gcggtgccgg aacctcagaa tcgccgctgg gtgcaatatt gcatacggta gacggcacac
    22381 ccggcgagac ggtgatcgaa gcgaaagaac tgaccaagaa atttggtgat tttgccgcca
    22441 ccgatcacgt caactttgcc gttaaacgtg gggagatttt tggtttgctg gggccaaacg
    22501 gcgcgggtaa atcgaccacc tttaagatga tgtgcggttt gctggtgccg acctctggtc
    22561 aggcgctggt gctggggatg gatctgaaag agagttccgg taaagcacgc cagcatctcg
    22621 gctatatggc gcaaaaattt tcgctctacg gcaacctgac ggtcgaacag aatttacgct
    22681 ttttctctgg tgtgtatggc ttacgcggtc gggcgcagaa cgaaaaaatc tcccgcatga
    22741 gcgaggcatt cggcctgaaa agtatcgcct cccacgccac cgatgaactg ccattaggtt
    22801 ttaaacagcg gctggcgctg gcctgttcgc tgatgcatga accggacatt ctgtttctcg
    22861 acgaaccgac gtccggcgtt gatcccctca cccgccgtga attttggctg cacatcaaca
    22921 gcatggtaga gaaaggcgtc acggtgatgg tcaccaccca ctttatggat gaagcggaat
    22981 attgcgaccg catcggcctg gtgtaccgtg ggaaattaat tgccagcggc acgccggatg
    23041 atttgaaagc gcagtcggcc aacgacgagc aacccgatcc caccatggag caagccttta
    23101 ttcagttgat ccacgactgg gataaggagc atagcaatga gtaacccgat cctgtcctgg
    23161 cgtcgcgtac gggcgctgtg cgttaaagag acgcggcaga tcgttcgcga tccgagtagc
    23221 tggctgattg cggtagtgat cccgctgcta ctgctgttta tttttggtta cggcattaac
    23281 ctcgactcca gcaagctgcg ggtcgggatt ttactggaac agcgtagcga agcggcgctg
    23341 gatttcaccc acaccatgac cggttcgccc tacatcgacg ccaccatcag cgataaccgt
    23401 caggaactga tcgccaaaat gcaggcgggg aaaattcgcg gtctggtggt tattccggtg
    23461 gattttgcgg aacagatgga gcgcgccaac gccaccgcac cgattcaggt gatcaccgac
    23521 ggcagtgagc cgaataccgc taactttgta caggggtatg tcgaagggat ctggcagatc
    23581 tggcaaatgc agcgagcgga ggacaacggg cagacttttg aaccgcttat tgatgtacaa
    23641 acccgctact ggtttaaccc ggcggcgatt agccagcact tcattatccc cggtgcggtg
    23701 accattatca tgacggtcat cggcgcgatt ctcacctcgc tggtggtggc gcgagaatgg
    23761 gaacgcggca ccatggaggc tctgctctct acggagatta cccgcacgga actgctgctg
    23821 tgtaagctga tcccttatta ctttctcggg atgctggcga tgttgctgtg tatgctggtg
    23881 tcagtgttta ttctcggcgt gccgtatcgc gggtcgctgc tgattctgtt ttttatctcc
    23941 agcctgtttt tactcagtac cctggggatg gggctgctga tttccacgat tacccgcaac
    24001 cagttcaatg ccgctcaggt cgccctgaac gccgcttttc tgccgtcgat tatgctttcc
    24061 ggctttattt ttcagatcga cagtatgccc gcggtgatcc gcgcggtgac gtacattatt
    24121 cccgctcgtt atttcgtcag caccctgcaa agcctgttcc tcgccgggaa tattccagtg
    24181 gtgctggtgg taaacgtgct gtttttgatc gcttcggcgg tgatgtttat cggcctgacg
    24241 tggctgaaaa ccaaacgtcg gctggattag ggagaagagc atgtttcatc gcttatggac
    24301 gttaatccgc aaagagttgc agtcgctgct gcgcgaaccg caaacccgcg cgattctgat
    24361 tttacctgtg cttattcagg tgattctgtt cccgttcgcc gccacgctgg aagtgactaa
    24421 cgccaccatc gccatctacg atgaagataa cggcgagcat tcggtggagc tgacccaacg
    24481 ttttgcccgc gccagcgcct ttactcatgt gctgctgctg aaaagcccac aggagatccg
    24541 cccaaccatc gacacacaaa aggcgttact actggtgcgt ttcccggctg acttctcgcg
    24601 caaactggat accttccaga ccgcgccgtt gcagttgatc ctcgacgggc gtaactccaa
    24661 cagtgcgcaa attgccgcca actacctgca acagatcgtc aaaaattatc agcaggagct
    24721 gctggaagga aaaccgaaac ctaacaacag cgagctggtg gtacgcaact ggtataaccc
    24781 gaatcttgac tacaaatggt ttgtggtgcc gtcgctgatc gccatgatca ccactatcgg
    24841 cgtaatgatc gtcacttcac tttccgtcgc ccgcgaacgt gaacaaggta cgctcgatca
    24901 gctactggtt tcgccgctca ccacctggca gatcttcatc ggcaaagccg taccggcgtt
    24961 aattgtcgcc accttccagg ccaccattgt gctggcgatt ggtatctggg cgtatcaaat
    25021 ccccttcgcc ggatcgctgg cgctgttcta ctttacgatg gtgatttacg gtttatcgct
    25081 ggtgggattc ggtctgttga tttcatcact ctgctcgaca caacagcagg cgtttatcgg
    25141 cgtgtttgtt tttatgatgc ccgccattct cctttccggt tacgtttctc cggtggaaaa
    25201 catgccagta tggctgcaaa acctgacgtg gattaaccct attcgccact ttacggacat
    25261 taccaagcag atttatttga aggatgcgag tctggatatt gtgtggaata gtttgtggcc
    25321 gctactggtg ataacggcca cgacagggtc agcggcgtac gcgatgttta gacgtaaggt
    25381 gatgtaactt cttatctttc gccagcaaag acactaccgc cgggccggca aggattgcca
    25441 gccctgcaat tgccagcaaa aagttgtttt ccagtacacg ggacaacagc cacagcacaa
    25501 tcagcgctgc gccaaaatac caggtcgtgg tcagctcttc cagcacgtct gccacctcac
    25561 gccagcgttg ctcggggtgg cgctgaaaca ccaacatcat cactaccgtg acacaataca
    25621 gtgcgcataa tcccacgccg acgtgaacca gagtcttact catccagccc accaccagca
    25681 gcgccactac cagtaaatgc aacataatct gccagcaact tagaccagtt gcgacacgaa
    25741 cacgttgttg ccacttcatg gcttctctcc tgaagaattt atctctactt actcagagta
    25801 ccgaacttta cggaaaattc cgtaagaccg cacctttttg tgacgccgac tacactattt
    25861 caatgagata agaggcaaag acaggaaaat tatgcccgat caaacacaac aattttcgtt
    25921 caaggtgctc accatcaaca ttcacaaagg ttttaccgcg tttaaccgac gcttcatttt
    25981 gccggaactt cgcgacgcgg tgcgtaccgt cagcgccgat attgtttgcc tgcaggaagt
    26041 gatgggcgcg cacgaagttc atccgctgca tgtggaaaac tggcccgata cctcgcacta
    26101 cgagtttctc gccgacacga tgtggagcga ttttgcctac ggtcgcaatg ccgtataccc
    26161 ggaagggcat cacggcaacg ccgtactgtc gcgttatccc attgaacatt atgagaatcg
    26221 cgatgtttcg gtcgatggtg cggaaaagcg cggcgtgctc tactgccgca ttgtgccacc
    26281 gatgaccgga aaagcgattc atgtgatgtg cgtacatctg ggcctgcgtg aggcgcaccg
    26341 tcaggcgcag cttgcgatgc tcgccgaatg ggtgaatgag ctaccggacg gcgaaccggt
    26401 attggtggcg ggtgatttca atgactggcg gcaaaaagct aatcatccgt taaaagtgca
    26461 ggccggactg gatgagattt ttacccgcgc ccacggacgc ccggcgcgca cgtttccggt
    26521 gcaatttcct ctactacgac tggacaggat ctacgtcaaa aatgccagcg ccagcgcgcc
    26581 aaccgcgttg ccgctgcgga catggcgaca cctttctgat catgcccctt taagtgcgga
    26641 gattcattta tgaaatgtag ctggcgcgaa ggcaataaga tccagttgct ggaaaacggc
    26701 gagcaatatt atcccgcggt gtttaaggcg attggcgagg cacaagaacg catcattctt
    26761 gaaacgttta tctggtttga ggatgacgtc ggcaaacagc tgcatgcggc actactggca
    26821 gcagcgcaac gcggggttaa agcggaagtc ttgctggatg gctacggttc gccggatctc
    26881 agcgatgagt ttgtcaatga actgacggca gctggcgtgg tatttcgcta ctacgatccc
    26941 cgccctcgcc tttttggtat gcgcaccaat gtgtttcgcc ggatgcatcg caaaattgtg
    27001 gtgatcgacg cgcgtatcgc ctttattggc gggctgaatt actccgccga gcatatgtcc
    27061 agctacggtc cagaggctaa acaggattac gcggtacgcc ttgaagggcc gattgtcgaa
    27121 gatattctcc agtttgagct ggaaaacctg cctggacaga gcgcggcacg acgctggtgg
    27181 cgacgtcatc acaaagcgga agagaaccgc cagccgggag aagcgcaggt attgctggtc
    27241 tggcgcgata acgaagaaca tcgcgatgat attgaacgtc actatctgaa aatgctcact
    27301 caggcgcggc gagaagtcat tatcgccaac gcctacttct tccccggcta tcgattttta
    27361 cacgccttgc gtaaagcggc acggcgcggg gtgcggatca aactgatcat tcagggcgaa
    27421 ccggatatgc cgattgtcag agtcggtgcg cgcttgctgt ataactatct ggttaaaggc
    27481 ggcgttcagg tatttgagta ccgccgccgc ccgctccacg gcaaagtggc attgatggac
    27541 gatcactggg cgacagtagg gtccagtaat ctcgatccgc tcagtttgtc actgaatctc
    27601 gaagcaaatg tcatcatcca cgatcgtcat tttaaccaga cgctgcgcga taatctgaac
    27661 ggcattattg ccgcagattg tcagcaggtc gatgagacca tgctgccgaa acgcacctgg
    27721 tggaacctga ccaaaagcgt gctggcgttc cactttttac gccacttccc ggcgctggtt
    27781 ggctggcttc cggcacacac accacgtctg gcgcaggttg atccgcccgc acaaccgaca
    27841 atggaaacgc aggatcgggt agaaactgaa aacacggggg taaaaccctg atgagtaaat
    27901 cacacccgcg ttggcgctta gcaaagaaga tcctcacctg gctgtttttt atcgcggtga
    27961 ttgtgttgct ggtggtctac gccaaaaaag tggactggga agaggtctgg aaggtcatcc
    28021 gcgactacaa tcgcgttgcg ctgcttagtg cggtcgggct ggtggtcgtc agctatctga
    28081 tttacggctg ctatgacctg ctcgcccgct tttactgcgg tcacaaactg gcgaagcgcc
    28141 aggtgatgct ggtgtcgttt atctgctacg ccttcaacct gacgctcagt acctgggtcg
    28201 gcggcattgg tatgcgctat cgtttgtact cccgactagg gttaccgggc agcactatta
    28261 cgcggatttt ctcgctcagt attaccacca actggctggg ctatatttta ctggcaggga
    28321 ttatctttac cgcaggcgtg gtggagttgc cggatcactg gtatgtcgat caaactacgc
    28381 tgcgtattct cggcattggc ttactgatga ttatcgcggt ttatttgtgg ttttgcgctt
    28441 tcgcgaagca ccgccatatg accatcaaag ggcaaaagct ggtgctgcct tcatggaaat
    28501 tcgccctcgc tcagatgctg atttccagcg ttaactggat ggtaatgggg gcgattatct
    28561 ggctgttact tggtcaaagc gtgaactatt tctttgtact gggcgtgttg ctggttagta
    28621 gtattgctgg tgtcatcgtg catattccgg cgggaattgg tgtgctggaa gcggtgttta
    28681 tcgcgctact ggctggggag catacatcca aaggcacaat tattgccgcc ctactcgctt
    28741 atcgcgtcct gtattacttt atcccgctgc tgctggcgct gatctgttac ctgctactgg
    28801 aaagccaggc gaagaagcta cgggcgaaaa atgaagcggc gatgtgaagt ttagttaagt
    28861 tctttagtat ctgcatttac gactaatgaa aaaaacgcgt atgcctttgc cagacaagcg
    28921 ttatagctat cgcggcaagt atcccaaaga agccgataaa atagtaatag agtgttaatg
    28981 cacctgtaac agcgcaacga accggactcc acaactcttc actatggcat ttccccgcca
    29041 attcaaggag cttatttcgt ccatgcgaga taatgcctga ccataacaca atcattaagc
    29101 aacttattac ccaaacgggt cgttcactca ttaatgcggc attcatgatg atcaccaggg
    29161 ccaaccctgt caacgtcatc ataatgataa aacggtgaga accaggatca acattaaata
    29221 ataaaccggc cagcatactg attaaaaaca tcgcgcccgc agtgctgaac acgacgataa
    29281 tggcaggtac aggagctaaa ctagcaacga caatcgccac caaagagagt aaaaatagat
    29341 acattacgcc tgcgatgacg gcatttatct tgttgatatc cttacttaag ccatacatac
    29401 aaagtaataa taatcctatg gcgacataca tttgcggtga atcatatttt gttgccgtaa
    29461 tttcgagata cttcgctacc caggccagtc ctgcggttgt caccaaacct aaagcggtaa
    29521 tgagccatat accgtttaat atcctggtct cgtgctgaaa atctaattta ttgctgtttt
    29581 gtgagtatga ttccattctt ttagttgtcc ttagaaaaaa tattaatatt caccatcaat
    29641 aaatttaaaa ccacgcatac aaaatacttt tgtatttatg gatacggaaa acgcttccgc
    29701 tcgccataag gcgtatttta tacctggtac ttgttaagtg tcgtttttag taagaaaaaa
    29761 aatcccgaca catggccggg atattgatgg tgaaaaagaa ttaacggcgg ttgccaaaaa
    29821 ttcgcagcaa catcaggaac aggttgatga agtccagata caaggttaac gcgccaagaa
    29881 tggaatattt gcgcaggttc gacgtgtcgc gggtatcaat ctgctcaccc atatttttca
    29941 atttctgagt gtcatacgcc gtcaatccga caaagacaat cacgccgatg taggtaactg
    30001 cccacatcaa tgcttcgctt ttcagccaga agttgaccag cgatgccagc acaatgccga
    30061 ttaacgccat aaacagcata ttgccaaagc cacttaaatc gcgcttcgtg gtgtaaccgt
    30121 acaggctcat tgcgccgaac atcccggcag taacgacgaa agtactggcg atagaagcag
    30181 cggtatagac aatgaatata ctggaaagcg taagacccgt cagcgccgaa taaagcataa
    30241 agagcatcgt cgttacacct gcgctcagct tttgaatcat cgctgataac acaataacca
    30301 atgctaattg cgcgatgatc agaccgatta aaaagacacg gttagtaaac aacagctcca
    30361 tcacggccgc ggaattagcc gcataccagg caacaaatgc ggtcagcaac aagccaacgg
    30421 tcatccagcc atagacttgc gccatataag tttgcaagcc agcccggggt tgtacgattg
    30481 aatcagaacg tgggaatctg tccatgacga tctcctgaag atataaggaa tatcttaagg
    30541 atactgcaaa atgatgaggc tgtgcatcga cgcagcgtaa acgcatgtac tgagcggtga
    30601 aattgccgga cgcagcggtg ccttatccgg ctaacaaaaa actaccagcg ttttgccgcc
    30661 tgctgatcgc tctcccgagc ttcaacccag cggtcgcctt ccggcgtggc ttcgcgcttc
    30721 cagaacggtg cgcgggtttt gagataatcc ataataaact gcccggcttc aaacgcactg
    30781 ctgcgatgcg cactggtgac accgacaaaa acgatttcat cgcccggcca taattccccg
    30841 atgcggtgaa tcacagtgac gcgccccagc ggccagcggt tacgcgcttc atcaacaatt
    30901 tctgccagtg ctttttcagt catccccgga tagtgttcga gggttaatgc tttgacgctg
    30961 tcgcccaggt tatggttgcg caccttacca gtaaaggtga ctaccgcacc gtcttcgtca
    31021 cgctccgcca gccacgggta ctcttctcct acgctgaacg gctgcggacc aacaacaatt
    31081 ttggtttctg ccatcttaac ctccggttac cggcgggaag aaagctactt cgtcgccgtc
    31141 agtcagcgga tggtcaaaac tcaccagcgt ctggttgacg gcagccagta atttgccatc
    31201 ttccagcgcc agcgcccagc gatcgctctg cgcagccatg tgctggcgta acgcttcaac
    31261 agttgggaaa tccgcagcca cttcggttgc atctgttccc accaactcgc gcacctgggc
    31321 gaaaaaaaga actttaatca tccgcttcca ccttaaagtc acccgacttg ccgccgctct
    31381 tcgccagcaa acgtaccgga ccaatcacca tatctttttg caccgctttg cacatgtcat
    31441 aaatggtcag cgccgccacg gaggccgcgg ttaatgcttc catttcgaca ccggttttcc
    31501 cggtcaggcg gcataaggtt tctatacgca cccgattgtg ctccggctcg gcctgtaaat
    31561 tgacttcaac tttgctgagc atcagcggat gacagagcgg gatcagatcc caggtgcgtt
    31621 ttgccgcctg aataccggca atacgcgcag tggcaaatac gtcgcctttg tggtggcgac
    31681 catcaataat catcgccagc gtctcgctgc gcatggtgac aaaggcttcc gcccgcgctt
    31741 cacgcacggt ttccgctttg gcggagacat ccaccatgtg cgcttcgcca gcggcgttga
    31801 tatgggtcag ttgcgacata cttatttctt caaatgtgga tggaaattac acggacgcgt
    31861 acgggcatcc agctgcggcg cgatgatatt ttcccatgcg gtacggcacg ctttggtcga
    31921 acccggcatg gcgaaaatca gcgttttgtt ggcgacgccc gctaccgcac gagattgcaa
    31981 cgtggaagtg ccaatctctt caaacgacaa catacggaac acttcaccaa aaccttcaat
    32041 ttcacggtcg aacaacggca gcaatgcttc gggagcctga tcaccttccg tcaggccagt
    32101 accacccgta atcaatacca cttgtacatc gtcgctggcg atccacgcag atacctgagc
    32161 gcgaatagcg tagcggtttt ctttcacaat ggctttatcg acaacgtgat ggcccgcttc
    32221 ttgcgccgaa tcgcgcagat agtgaccgga ggtatcgtct tcttcaccgc gacgattaga
    32281 aaccgtaaga atagcaatac gggtcgggat aaattcagtg cttacctgac tcatctgatc
    32341 tctccttttg acgttttagc cgccaatgta cgataagttt tgcgtaatac cggtgttgtt
    32401 ttgatgcagg aaatgggtct gtttcttctc ccgcagcgcc gctgaaatac gcgcttccag
    32461 cgcctgttgc tgggtatcgt cttccagcag atcgcgcagg ttaacgccgc cttcaccaaa
    32521 caggcagaga tggagtttac caatggagga aacgcgcagg cggttgcaag tggcgcagaa
    32581 gtctttttca tacggcatga taaggccaat ctctccggcg taatctggat ggcaaaagac
    32641 ttgcgcggga ccgtcgctgc gttgacgtaa ttggtggatc cagccgcgac gcagtagctc
    32701 gtcacgcaga acctgaccag agatgtgatg cttacggaag agctcgctgc cctcgcccgt
    32761 ttccatcagt tcgatgaaac gcagctggat agggcgatgc tggatccagt tcagaaaggt
    32821 gtcgagctgg tgatgattaa catcacgcat cagcacggta ttgactttga ccttctcaaa
    32881 accggcctca aatgcagcat caatccccgc catgacctgg ttgaatttat cctgcccggt
    32941 aatagcgtga aactggcggg cgtccagact gtcgacactg acgttaatgc cagtaagtcc
    33001 cgcatcgcgc cagttcgcca catcgcgttc cagacggtaa ccattggtgg tgaccgcaat
    33061 ctggcggata gcgtcgtttt cccgcacagc ggcgatgata tcggtaaagt cgcggcgtaa
    33121 agacggctca ccgcctgtca ggcgcacttt ttcggtgccc agactggcga aggcgcgcgt
    33181 aacccggcga atttcatcga cggtaagaaa gcctttattg gtgacgccgc tcggtttgta
    33241 gccatccggc aggcagtagg tgcaacgaaa gttacacaca tcggtaatcg acaggcgcaa
    33301 gtagtaaaac ttacgcgcaa atgcatcagt cagttgtgaa gccatgtaca cctttccaga
    33361 tacgggaggc gaagtcattt cttccttcgc cctggtggca atctttacga gcattgtcac
    33421 ggccaaagca ccgtatcagt tgacccaggt gcagaggcta gagtgtttag tggttatgcc
    33481 gatactagcg tgtaaatgag atttttacca tccacacttt cgctatgtaa tcatgtatat
    33541 agcatcatga tcgtgctatt tcgctacaga ataagggaaa atggcggctt tactttgata
    33601 tacatcatca gggaggcata gcgtgcatcg tcctttagag gtagaagaaa atagtttgta
    33661 tcctcaatat tggcaggtta attgctgttt ccccgcaatt tgcgctactg tagcgcggat
    33721 aatctgactc caggaaacta tatgcgcaat cgtacgctgg ctgatcttga tcgtgtcgtt
    33781 gctctcggcg gagggcatgg actgggacgc gttctctcat cactttcgtc tttgggttct
    33841 cgtttaacgg gtatcgtcac caccaccgat aatggtggct cgacggggcg tattcgccgt
    33901 tcagaaggcg gcattgcctg gggcgatatg cgcaactgcc tcaaccagct gataacggaa
    33961 ccgagcgtcg cctccgcgat gtttgaatac cgttttggtg gcaatggcga actttccggt
    34021 cataatctcg gaaacttgat gttaaaggcg ctggatcacc ttagcgtgcg gcctctggaa
    34081 gccatcaatt taattcgtaa tctgctgaaa gtggatacgc atttgattcc aatgtcagag
    34141 catcctgttg atctgatggc gattgacgat caggggcatg aagtttacgg cgaggtcaat
    34201 atcgaccagt taactacgcc gattcaagag ttattgttaa cgcctaatgt acccgcaacg
    34261 cgtgaggcgg ttcacgctat caatgaagcg gatctcatca ttattgggcc tggcagtttt
    34321 tataccagcc tgatgccaat tctgctgctg aaggaaatcg cccaggcatt acgccgcacg
    34381 ccagcgccga tggtttatat cggcaatctg gggcgtgagt tgagtttacc tgcggctaat
    34441 ttgaagctgg aaagcaagct ggcaattatg gagcagtatg ttggtaaaaa ggtcattgat
    34501 gcggtcatcg tcgggccaaa agtggatgtc tcggcggtga aagagcggat tgtgatccag
    34561 gaggtactgg aggccagcga tattccgtat cgtcatgacc gccagttgtt acataacgcg
    34621 ctggaaaagg cgttacaggc tttaggttaa cggaaatccg aaggaaaatt ccggcttcct
    34681 attgaagaca aagtgcgcgt tgtttatgcc ggatgcggcg tgaacgcctt atccggccta
    34741 caaaccgcgc aaattcaata tattgcggag aaaatgtagg cctgataagc gtagcgcatc
    34801 aggctgtttt ccgtttgtca tcagtcttct tcgctatcct gttacgatgc cgcgataaac
    34861 agctcacgca gctgatgcaa ctggtcacga atttgcgccg cttcttcgaa ctccagattc
    34921 tgcgcgtgtt gcatcatcaa cccttccagc tcatggattt tctgctgcaa cgctttaggc
    34981 gacatatcca tcggcacatt atccggctca acaatcggtc gcgattttcc tctgcccttc
    35041 gctttggttt tagcaatgtt ctgccccaac gccaggatat cgaccacttt cttgttcaag
    35101 ccttgcggcg taatgccgtg ttcctcgttg tacttctgct gtttctcgcg gcgacgttcg
    35161 gtttcgccaa tcgctttcgc cattgatggg gtgatcttat cgccgtagag aatcgcttta
    35221 ccgttaacgt tacgtgccgc acgaccaatg gtctggatca acgaacgttc ggaacgcagg
    35281 aagccttctt tgtcagcgtc gaggatcgcc accagcgaaa cttccggcat atccagacct
    35341 tcacgcagta agttgatccc taccaatacg tcgaactcac ccagacgcaa gtcgcggata
    35401 atctccatac gctcgacggt gtcgatatct gagtgaagat aacgcacgcg ctcaccgtgt
    35461 tcttcgagat attcggtaag atcttccgcc attcgcttgg tcagtgtggt gaccagtacg
    35521 cgttcgttaa ttgccgctcg ctgacgaatc tccgaaagaa gatcatcaac ctgtgtcgcc
    35581 accggccgca cttcgataat cgggtcaagc aatccggttg gacgcaccac ctgatccacc
    35641 acatcgccgc cggatttttc cagctcgtaa ttacccggcg tcgccgaaac atagatggtt
    35701 tgcggcgcta atgcttcgaa ctcttcaaac ttaagcggac ggttatccag cgctgatggc
    35761 aggcggaagc cgtactccac cagtgtctct ttacgcgccc ggtcaccgcg atacatgccg
    35821 ccaatttgtg gaatggtgac gtgagattca tcgaccacca gcagcccatc agcagggagg
    35881 taatcaaaca gcgtcggcgg tggctcaccc ggtccacgac cggagaggaa gcgcgagtag
    35941 ttttcaatcc ccgaacagta gcccagctcg ttcatcatct ccagatcaaa ctgggtacgc
    36001 tgggtcagcc gctgctcttc cagcagtttg ttgttttcca acagcacttt gcgtctggcg
    36061 gccagctctt ctttgatctc ctccatcgcc tgtacgatgc gctcgcgcgg tgtgacgtag
    36121 tgcgttttcg ggtagatggt aaaacgtgga atagtggaaa caatctgccc ggtcagcggg
    36181 tcaaataacg acaatcgttc cacttcctcg tcaaacagtt ccacgcgaag tgcaatgtca
    36241 tccgattctg ccgggaagat atctatcacc tcgccacgaa cgcggaaagt accacgctgg
    36301 aatgcttgat cattacgagc gtattgcagc tccgccagtc ggcgcagaat cgcgcgctga
    36361 tcgataatca taccgaccgt gagatggagc atcatcttga gatataaatc aggatcgccc
    36421 agaccataaa tcgcggaaac agacgccacc acaaccacat cacgccgctc cagcatcgct
    36481 ttggtggcgg acaaacgcat ctgctcaata tgttcgttaa ccgaggcatc tttctcaatg
    36541 aaagtgtcgg aactcggtac ataggcttcc ggctgatagt agtcgtagta ggaaacgaaa
    36601 tattccaccg cgttttccgg gaagaactct ttcatttcgc catacagctg ggccgccagc
    36661 gttttgttgg gcgcaagtac catggttggg cgctgaaggt cagcaatgac attggcaatg
    36721 gtgaaggttt tcccggagcc agtcacgcca agtaacgtct ggtgcgccag gccatcttcc
    36781 agcccctctt cgagacgtcg aatcgcctct ggctgatcgc cagaaggttt aaaagcggaa
    36841 ttcagtttga acggtttact catgagtcgc tacctgaagg agttgggcgg gcaggtatgt
    36901 aattttactc gtcgtactta attatgccaa caaattatac tggataaaaa aacagttcat
    36961 caccataata tttctgttac agcgtaaact ccgctgtcaa tcatgagcaa aatttactct
    37021 gtggcgagat aaaactccgg ccttaccggg ttatccccaa agcaacggct tttttaacat
    37081 ttgtcaagat gagtgatgac agttttatgg caagagatgc ctgttcagtg actgccattg
    37141 catttatcta accagttaaa aaataaaaga tatttctttg agccgtcttt aacgccaatt
    37201 cgcgtgcaag ccgcgtattc tctcgcttgc ctcgtgtttt ctaactctaa ta
//
LOCUS       DAJOWB010000035        35888 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJOWB010000035 DAJOWB010000000
VERSION     DAJOWB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.035"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(124..2391)
                     /gene="adiA"
                     /locus_tag="OHO85_001934"
     CDS             complement(124..2391)
                     /gene="adiA"
                     /locus_tag="OHO85_001934"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCQ2671310.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHSDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2590..3498)
                     /gene="melR"
                     /locus_tag="OHO85_001935"
     CDS             complement(2590..3498)
                     /gene="melR"
                     /locus_tag="OHO85_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCQ2671311.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3781..5136
                     /gene="melA"
                     /locus_tag="OHO85_001936"
     CDS             3781..5136
                     /gene="melA"
                     /locus_tag="OHO85_001936"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCQ2671312.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5239..6660
                     /gene="melB"
                     /locus_tag="OHO85_001937"
     CDS             5239..6660
                     /gene="melB"
                     /locus_tag="OHO85_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCQ2671313.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6799..7428)
                     /locus_tag="OHO85_001938"
     CDS             complement(6799..7428)
                     /locus_tag="OHO85_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCQ2671314.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GAGDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7551..9197)
                     /gene="fumB"
                     /locus_tag="OHO85_001939"
     CDS             complement(7551..9197)
                     /gene="fumB"
                     /locus_tag="OHO85_001939"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCQ2671315.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKSQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9275..10615)
                     /gene="dcuB"
                     /locus_tag="OHO85_001940"
     CDS             complement(9275..10615)
                     /gene="dcuB"
                     /locus_tag="OHO85_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCQ2671316.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11186..11905)
                     /gene="dcuR"
                     /locus_tag="OHO85_001941"
     CDS             complement(11186..11905)
                     /gene="dcuR"
                     /locus_tag="OHO85_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCQ2671317.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11902..13533)
                     /gene="dcuS"
                     /locus_tag="OHO85_001942"
     CDS             complement(11902..13533)
                     /gene="dcuS"
                     /locus_tag="OHO85_001942"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCQ2671318.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13714..13944
                     /locus_tag="OHO85_001943"
     CDS             13714..13944
                     /locus_tag="OHO85_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCQ2671319.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            13956..14228
                     /locus_tag="OHO85_001944"
     CDS             13956..14228
                     /locus_tag="OHO85_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCQ2671320.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            14455..14751
                     /gene="ghoS"
                     /locus_tag="OHO85_001945"
     CDS             14455..14751
                     /gene="ghoS"
                     /locus_tag="OHO85_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCQ2671321.1"
                     /translation="MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            14779..14952
                     /gene="ghoT"
                     /locus_tag="OHO85_001946"
     CDS             14779..14952
                     /gene="ghoT"
                     /locus_tag="OHO85_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HCQ2671322.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(15071..16588)
                     /gene="lysS"
                     /locus_tag="OHO85_001947"
     CDS             complement(15071..16588)
                     /gene="lysS"
                     /locus_tag="OHO85_001947"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCQ2671323.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(16825..18282)
                     /gene="dtpC"
                     /locus_tag="OHO85_001948"
     CDS             complement(16825..18282)
                     /gene="dtpC"
                     /locus_tag="OHO85_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HCQ2671324.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMVKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(18341..20488)
                     /gene="cadA"
                     /locus_tag="OHO85_001949"
     CDS             complement(18341..20488)
                     /gene="cadA"
                     /locus_tag="OHO85_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HCQ2671325.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(20568..21902)
                     /gene="cadB"
                     /locus_tag="OHO85_001950"
     CDS             complement(20568..21902)
                     /gene="cadB"
                     /locus_tag="OHO85_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HCQ2671326.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(22268..23806)
                     /gene="cadC"
                     /locus_tag="OHO85_001951"
     CDS             complement(22268..23806)
                     /gene="cadC"
                     /locus_tag="OHO85_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HCQ2671327.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(24610..26145)
                     /locus_tag="OHO85_001952"
     CDS             complement(24610..26145)
                     /locus_tag="OHO85_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCQ2671328.1"
                     /translation="MFSRLFVKKHTPAMVAGSLIFLFIILLTFFWKKAELVNDSQIRV
                     NFALGYIENILNQNNSISQEAEHLLLNNCNADTQHELSNLLLKRPQLRALSLARQEAV
                     FCSTHPGLPVGPVAEKEQWRHDMLIRFPEDTGTLPWILLRTPYKNGTVITATDYYFIQ
                     DIISVVHAVPAIRFRLGNTVLSASGKNVTLLPDDSGIQKESHSKKYPFSLIYIIPVKM
                     QLTYAWKQAWYMIPVAIFGGILTAFLLSRRRPSSPLDMLKNALAHGEFRPYFQPIISA
                     KNHQLTGCEVLIRWHHPVSGIIPPDQFIPLAESTGLIAPITQQLMSQVEHSLNSVAHF
                     LPNQFHIGINISPAHFLSPGLEDSCMKFLSTFPKGKVKLVLELTERNQLSVTAESKAL
                     FAKLHQQGVLFALDDFGTGYATHSYLQSFPVDYIKLDKGFVQMVGVDEISGHIVENVI
                     ELARRLGIDIIAEVIETESQELFMTEKGSCYLQGYRYAPPLPAEQFIAEWVYNSGNGK
                     NQR"
     gene            complement(26216..27061)
                     /locus_tag="OHO85_001953"
     CDS             complement(26216..27061)
                     /locus_tag="OHO85_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCQ2671329.1"
                     /translation="MSDITISRPEVVTGHTDVICSTSIRHVVTVRNAALQQTETLIRQ
                     LAEISVLTAGIGGKTALDWAMKQNFRCGCWLMEKPEAAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNIDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLYLFSGKPIPDNR
                     ENITIHLDDHIHSVQGKACYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKYYPE
                     TLSPV"
     gene            complement(27146..27343)
                     /locus_tag="OHO85_001954"
     CDS             complement(27146..27343)
                     /locus_tag="OHO85_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2671330.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDVRAVRHLQLLHQNR"
     gene            complement(27355..27843)
                     /locus_tag="OHO85_001955"
     CDS             complement(27355..27843)
                     /locus_tag="OHO85_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2671331.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYL
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRCLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(27840..28217)
                     /locus_tag="OHO85_001956"
     CDS             complement(27840..28217)
                     /locus_tag="OHO85_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCQ2671332.1"
                     /translation="MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRNNYRMVNNITQGKHPEAKQ"
     gene            complement(28264..28641)
                     /gene="cbtA"
                     /locus_tag="OHO85_001957"
     CDS             complement(28264..28641)
                     /gene="cbtA"
                     /locus_tag="OHO85_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HCQ2671333.1"
                     /translation="MSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTITLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPAITV"
     gene            complement(28720..28941)
                     /locus_tag="OHO85_001958"
     CDS             complement(28720..28941)
                     /locus_tag="OHO85_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCQ2671334.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(29010..29486)
                     /locus_tag="OHO85_001959"
     CDS             complement(29010..29486)
                     /locus_tag="OHO85_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCQ2671335.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNKLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(29501..29986)
                     /locus_tag="OHO85_001960"
     CDS             complement(29501..29986)
                     /locus_tag="OHO85_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCQ2671336.1"
                     /translation="MKTVSQNTCTLSASVVTASENDQPQLVATLVPHEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDHLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECNAIMRII
                     D"
     gene            complement(30077..30895)
                     /locus_tag="OHO85_001961"
     CDS             complement(30077..30895)
                     /locus_tag="OHO85_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCQ2671337.1"
                     /translation="MQLASRFGHVNQIRRDRPLTREELMQVVPSVFGEDKHTSRSENY
                     TWIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMTETLLENMR"
     gene            complement(30985..31218)
                     /locus_tag="OHO85_001962"
     CDS             complement(30985..31218)
                     /locus_tag="OHO85_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCQ2671338.1"
                     /translation="MSDCHPVLLPEGPFSREQAVAVTTAYRNVLIEDDQGTHFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNSYLASEGILRQ"
     gene            complement(31224..31901)
                     /locus_tag="OHO85_001963"
     CDS             complement(31224..31901)
                     /locus_tag="OHO85_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2671339.1"
                     /translation="MNTRALFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLSSRISINGNLIPTSQMCNQQTGFRAVEVEIGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARQAYAREYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVTVTVGADGNATGA
                     SVEFIGK"
     gene            complement(32049..32729)
                     /locus_tag="OHO85_001964"
     CDS             complement(32049..32729)
                     /locus_tag="OHO85_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HCQ2671340.1"
                     /translation="MSDNRSRHDRLAVRLSLIISRLMTGESLSLKTLSDEFGVTERTL
                     QRDFHQRLVHLDLEYRNGRYSLRRQSSPGAIPEMLSFIQNTGIARILPLRNGRLITCL
                     TDNQEPSPCLIWLPAPDITATFPECFSQLILAIRQCIHISLMAERWYPSLEPCRLIYY
                     SGSWYLIALQKGKLQVFPLADIKSVSLTSERFERRGHIHSLVAEERFISALPHFSFIH
                     KLINTFNL"
     gene            complement(32932..33816)
                     /locus_tag="OHO85_001965"
     CDS             complement(32932..33816)
                     /locus_tag="OHO85_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCQ2671341.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHSVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQLGERRMTLVDLPGIGETPQH
                     DQEYRTLYRQLLPELDLIIWILRADERAYAADITMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNATEKCPSRQQALSLATVTARVATLFPSSFPVLSVAAPVGWNLPAFVSLMI
                     HALPPQATSAVYSHIRGENRSEQARKHAQQTFGETIGKSFDDAVARFSFPAWMLQLLR
                     KTRDRIIHLLITLWERLF"
     gene            33922..34884
                     /locus_tag="OHO85_001966"
     CDS             33922..34884
                     /locus_tag="OHO85_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2671342.1"
                     /translation="MTAQQSDALREIANKARVTTILQCKAWKDTQRILKRSGLVCRER
                     SEPFDPEKHFDCYTVRYLYLLNIMALELKSDTRIKVEVGQWYRMTGKRLSLNVPPFML
                     IPRNIRRKVDGFRQSRQSEDEATKNPPQPFTGSLYKVLSRDSDSAELDAWFAEPPLTR
                     QEVWEGRRVTDFDPWALSSFICRSESPTFELFYQEYKRLGLKSLFVSGVMFEQFLTGL
                     SFRKYGDWVESQLLESLGNVMFFMLLYDMENLDKFIKELMDINVQSEDSKEKGKSRKE
                     RMLEYINSYIRNVYGRFLCTSKERYEQHKRKNSSKKKNGSGGTH"
     gene            complement(34881..35735)
                     /locus_tag="OHO85_001967"
     CDS             complement(34881..35735)
                     /locus_tag="OHO85_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory RepB family protein"
                     /protein_id="HCQ2671343.1"
                     /translation="MSVHSPDESDHVFSRMEYEYYRHNDKSPSVNADNAGRDVVPEWV
                     QKDPVAAFWVWMFIYHVPDSIVRLSGEQYADYGTSCLYARLFATEAAPANHQARIRVT
                     RKWLQELIRLTGTENVSDFLYVVHFLWSYAEKVNPMTWLGSRRIHIKWAWAYLQKHCT
                     DEERHCPDISEFKASGESRLFIIAYVRRVFMKIYSVGTEPQKLKELVYQQNDFLRRMK
                     CSYQKMFRYEKTDSRVQISALISPEARNALRKMAKKNNLTQAEMLDKLILSADRLFMQ
                     KTAGDYSR"
BASE COUNT         9108 a         8443 c         9002 g         9335 t
ORIGIN      
        1 ccgcgcaaaa agtatgacat ttttgtttgc agcaattttt atgaaaacgt tgccatactc
       61 aaaaggccta aggccaggcg gcgtaatgtt atttaaacaa ttacgccttc agcggaatag
      121 tggttacgct ttcacgcaca taacgtggta aataccgtca ataatttcag tcccttcagt
      181 ttcgtgttca aatccaggga aatggtggtc ccaggattgc agcgagcgta aataacttac
      241 ttgcggactg tttttatcgc cgaagttttc accagacagc agcatcggga ttcctggcgg
      301 atacgggata actgagtttg ccgcgatgcg tcctggcaga ttttcaatgg ataccagttc
      361 gacattgttg tcgacaatcg cgttgtacgc ttcacgcggg gtgatttccg ccatcggcag
      421 gccggaatag gcttcgttca accgtgcgcc agggttgttt tctttcagcc aggcaaacat
      481 ggtgtcaccc agatcgtgaa tccccatgtt cgcgtaagtg tcaggatatt gttcaacaag
      541 ttccggcatc acctgcgcca gcggtgtgtt ggcgtcatag tggcgtttga aggagcaaag
      601 ggtgttaacc agagtccccc atttcccacg ggttacgccc atagagaaca ggaacataat
      661 ttggaagtca gtggtgcggg taggcacaat gccgtggcga ccaagccagg cagtgaccag
      721 cgccgccgga acaccggttt cttccagttc accatcttca cccattcccg gagcaaggat
      781 gctgacttta attgggtcga gcatactcca gttatccgga atatctttga agccgtgcca
      841 gctttcgccc ggatgcatta cccagcagtc ctgaacggtg gtcagcagtt tggttggtgc
      901 gtcagcaaag tcataggttt tgccggtttg tgggtcggtg acgacttctt tgttccacgg
      961 tttgaagaac cagctaccgt cagcggtgaa ctctttatat agccgcgcca tcgcctgacg
     1021 gaaatcaacc gcttcgtcaa tcacttcctg tgtcagtgac aggccgctgt tgccgtccat
     1081 catcgacacc gccacgtcgt tggatgcgca gatggcatac agcggggagg tggtggcatg
     1141 catcatgtag gcctggttga agcgggagaa gttaatcgcc ccacgacctt cacgtacatg
     1201 aatataagaa gcctgtgaca gcgcattcag cagtttgtgg gtggagtggg tggcgaaaac
     1261 ggtaggaccg ttgtgatcgc caggttcgcc gcgcatggca tagtgatcgg catagatcgg
     1321 gttgaaacgt gcatagccgt accaggcttc gtcaaagtgc agacgatcgg aggttttttc
     1381 cagcagatcc tgcgcttctt tagcgttata acacacgccg tcataggtgc agttggtcac
     1441 cacgcagtaa gacggttttt gcccggcttt gtctttggtc agcgggcttt cactgatttt
     1501 cttctgcaag gtttcaggtt gcatttcctg cggatagatt ggcccgataa tgccgtagcg
     1561 gttgcggctt ggcaccatat agaccggttt cgcgcctgtc agcatcaagc cttgttcgat
     1621 ggatttatgg caattacggt caacgaccac gacatcgtta tcggtcatgc aagcctgcat
     1681 gatggtgcgg ttagagccgg aagtaccgac gactaccgac caggagcgat cggcaccaaa
     1741 tacgcgtgcg gcatattttt cgctttcgcc aaatgcgcca gtatggtcaa gcaaagaacc
     1801 gagggaagtt cgttcgatgc ccatgtcggt gcggaacaga ttttcaccat agtagtcatg
     1861 gtagaaacgt ccggcgggtg ttttggtaaa accaacgccg ccctggtggc ctggcgctgc
     1921 ccaggaatat tcatggatgt cactatattt catcagcgcg ctgaacagtg gcggcaacag
     1981 ctgctggcgg tagcgggtca tcgcggcaac ggcgcgtccg gcgataaagt cggcggtatc
     2041 ttccagaatc caggcgaatt catcgacaag ctccagcaga tcccggtcca ttgcggcgag
     2101 ggctttttcc cgatcgccca acaggaagac cggcacgttt tgttggcgct catgaagctt
     2161 accgatcaat tgtctgacgt tttgatgttc gtccgaatgt tccatttgat agctgaacat
     2221 caggcagtca atggcttcgt ttgaagagag aatggcaaaa ccatcatcaa aggaggtgga
     2281 tttaatcacg gtaacatttt gctggcttaa agcatctgcc agacgctcaa cggcgttacc
     2341 gacccaggtg tcttgatgga gaaactcgct ttcaacaatt aatactttca tcattgctta
     2401 cccggttatg aaggaatctt cgttgcgggc aagtatcttc cggccttttc gggctgtaaa
     2461 gcgcgtgaaa aacaaacatt tttgaaatat ttagttttat aaacaatacg ttatgtgatt
     2521 attttaaatt attttcactg cttattatcg acggctaaac tatttttttg gctgatactg
     2581 atatcgtctt tagccgggaa acgtctggcg gcgctgttgg ctaagtttgc ggtattgttg
     2641 cggcgacatg ccgacatatt tgccgaacgt gctgtaaaaa cgactacttg aacgaaagcc
     2701 tgccgtcagg gcaatatcga gaatactttt atcggtatcg ctcagtaacg cgcgaacgtg
     2761 gttgatgcgc atcgcggtaa tgtactgttt catcgtcaat tgcatgaccc gctggaatat
     2821 ccccattgca tagttggcgt taagtttgac gtgctcagcc acatcgttga tggtcagcgc
     2881 ctgatcatag ttttcggcaa taaagcccag catctggcta acataaaatt gcgcatggcg
     2941 cgagacgctg tttttgtgtg tgcgcgaggt tttattgacc agaatcggtt cccagccaga
     3001 gaggctaaat cgcttgagca tcaggccaat ttcatcaatg gcgagctggc gaatttgctc
     3061 gttcggactg tttaattcct gctgccagcg gcgcacttca aacgggctaa gttgctgtgt
     3121 ggccagtgat ttgatcacca tgccgtgagt gacgtggtta atcaggtctt tatccagcgg
     3181 ccaggagaga aacagatgca tcggcagatt aaaaatcgcc atgctctgac aggttccggt
     3241 atctgttagt tggtgcggtg tacaggccca gaacagcgtg atatgaccct gattgatatt
     3301 cactttttca ttgttgatca ggtattccac atcgccatcg aaaggcacat tcacttcgac
     3361 ctgaccatgc cagtggctgg tgggcatgat atgcggtgcg cgaaactcaa tctccatccg
     3421 ctggtattcc gaatacagcg acagcgggct gcgggtctgt ttttcgtcgc tgctgcacat
     3481 aaacgtatct gtattcatgg atggctctct ttcctggaat atcagaatta tggcaggagt
     3541 gagggaggat gactgcgagt gggagcacgg ttttcaccct cttcccagag aggcgagggg
     3601 agcgtccgag catcatgagg ccgaaaactc tgcttttcag gtaacttatt accataaact
     3661 cagatttact gctgcttcac gcaggatctg agtttatggg aatgctcaac ctggaagccg
     3721 gaggttttct gcagattcgc ctgccatgat gaagttattc aagcaagcca ggagatctgc
     3781 atgatgactg cacccaaaat tacatttatc ggcgctggtt cgacgatttt cgttaaaaat
     3841 attcttggtg atgtgttcca tcgcgaggcg ctgaaaacgg cgcatattgc cctgatggac
     3901 attgatccca cccgcctgga agagtcgcat attgtggtgc gtaagcttat ggattcagca
     3961 ggggccagcg gcaaaatcac ctgccacacc caacagaaag aagccttaca ggatgccgat
     4021 tttgtcgtgg tggcatttca gattggcggt tatgaacctt gcacggtgac tgatttcgag
     4081 gtctgtaagc ggcatggtct ggaacaaacc attgccgata cgttggggcc gggcggtatt
     4141 atgcgcgcgc tacgtaccat tccgcatctg tggcaaattt gcgaggacat gacggaagtc
     4201 tgccccgatg ccaccatgct caactatgtt aacccaatgg cgatgaatac ctgggcgatg
     4261 tatgcccgct atccgcatat caaacaggtc gggctgtgcc attcggtgca gggaacggcg
     4321 gaagagctgg cgcgtgacct caatatcgac ccagctacgc tgcgttaccg ttgcgcaggt
     4381 atcaaccata tggcgtttta cctggagctg gagcgcaaaa ccgccgacgg cagttatgtg
     4441 aatctctacc cggaactgct ggcggcttat gaagcagggc aggcaccgaa gccgaatatt
     4501 catggcaata ctcgctgcca gaatattgtg cgctacgaaa tgttcaaaaa gctgggctac
     4561 ttcgtcacgg aatcgtcaga acattttgct gagtacacac cgtggtttat taagccaggt
     4621 cgtgaggatt tgattgagcg ttataaagta ccgctggatg agtacccgaa acgctgcgtc
     4681 gagcagctgg cgaactggca taaagagctg gaggagtata aaaacgcctc ccggattgat
     4741 attaaaccgt cacgggaata tgccagcaca atcatgaacg ctatctggac tggcgagccg
     4801 agtgtgattt acggcaacgt ccgtaacgat ggtttgattg ataacctgcc acaaggatgt
     4861 tgcgtggaag tagcctgtct ggttgatgct aatggcattc agccgaccaa agtcggtacg
     4921 ctaccttcgc atctggccgc cctgatgcaa accaacatca acgtacagac gctgctgacc
     4981 gaagctattc ttacggaaaa tcgcgaccgt gtttaccacg ccgcgatgat ggacccgcat
     5041 actgctgccg tgctgggcat tgatgaaata tatgctcttg ttgacgacct gattgccgcc
     5101 cacggcgact ggctgccagg ctggttgcac cgttaaaacg cgactaaacg ctactgcgcc
     5161 gggggattta ttccggcgca cacctctgac gataccaata acagaaggcg ggcgttggta
     5221 acagcgaccc gataccctat gagcatttca atgactacaa aactcagtta tggatttgga
     5281 gcgttcggga aggattttgc gatcggcatt gtgtatatgt acctcatgta ttactacacc
     5341 gatgtcgttg ggctgtctgt gggtttggtc ggtactttgt ttctggtggc gaggatctgg
     5401 gatgctatta acgatccgat tatgggatgg attgtaaatg ctacgcgatc gcgatggggt
     5461 aagttcaaac cctggatcct gatcggtacg ttggcaaact ctgtaatctt atttctcctc
     5521 tttagtgcgc atctgtttga aggtactact cagattgtct ttgtttgcgt gacctacatc
     5581 ctctggggca tgacttacac cattatggat attcccttct ggtcgctggt tccaaccatc
     5641 acgctcgata aacgtgagcg cgaacaactg gttccttatc cgcgtttttt tgccagtctg
     5701 gcaggctttg ttacggcagg tgtgacgcta ccatttgtta attatgtcgg cggtggcgat
     5761 cggggatttg gctttcagat gttcactctg gtactaatcg ccttttttat tgtttcaacc
     5821 atcatcactc tgcgcaatgt gcatgaagtc ttttcgtcag acaatcaacc gtctgctgaa
     5881 ggaagccatc tgacacttaa agccatcgtt gcgctaattt ataaaaacga tcagctttca
     5941 tgcctcttgg gtatggctct tgcttataat gtagccagca acattattac cggatttgct
     6001 atctattatt tctcatatgt tatcggtgat gcggatttgt tcccctatta tctgtcgtat
     6061 gcgggagctg ctaacctggt gacgttagta ttcttcccac gcttagttaa atcattatcc
     6121 cgacgcattt tatgggccgg agcatctatt cttccggtgt taagctgtgg tgttctcctg
     6181 ttaatggcat taatgagcta tcacaacgtc gtcctcattg tgattgcggg tattttgctg
     6241 aatgtgggaa cggcgctttt ctgggtatta caggtcatca tggtggcaga taccgttgat
     6301 tacggtgaat ataaactgca cgtacgctgt gaaagtatcg cttactccgt gcagactatg
     6361 gtggtgaagg gcggttcagc ctttgcggct tttttcattg cggttgtgtt agggatgatt
     6421 ggctatgtac cgaatgttga acagtctacg caagccctat taggtatgca gtttattatg
     6481 attgctctac caactctgtt tttcatggta acgctgattc tctacttccg tttctatcgc
     6541 ctcaatggcg acacgctgcg caggatccag attcatctgc tggataaata tcgcaaagta
     6601 ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag tgagtgatgt gaaagcctga
     6661 cgtggacaat agctaacgaa aatggcctga tgcgatatgt ttatcaggcc acgtcttaat
     6721 gtaatgcttt ggatttgcaa attttgtaga ccggataagg aattcacgcc gcatccggca
     6781 tcaacaaagc gcacgttgtt atccggttat caagccaaag cgccgtagct ggcggcaatg
     6841 aaagcgagcg aggaatatca ccgtcgttaa tgcgccaagc agcgcgcaga acatatcgga
     6901 ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca cccgctccct ggcccattgc
     6961 cagcgccgcc caccactcga ttaattcata catcgcgctt atcgccagcg ccacgcagca
     7021 gaccaggaac gccaccattt tacgtccgcg cacgtacatc ccgcgcacga gaatttctcg
     7081 tgccaccagt gcaggcacca gcccctggaa aaaatgcccc agcttgtcat acggattacg
     7141 gctcaacccc agccattcct gcacctcaaa accgatgggg actttcgcgt aggtgtattg
     7201 cccgccgacc atcaggatga tggcgtgaaa gaaaatgagc gtatagagca gcggcgttaa
     7261 cggataacgt ttagcggtgg caagcagtag cggcacgaca ataatcaccg gtgtcacttc
     7321 catcagccag gtgagtttgt catgggccga aatgccggta taaatcagga ttaacgttag
     7381 cgccagtgcg ccggtgttaa gaattaacgg cttgagtgtg cgggtcatgg tgagttcgca
     7441 agtcagggaa aatactgact attcaccagc aagcgtgaaa ttacaatcgc aaaagaggtg
     7501 gcccaggggg atcacctggc agcatgctgc caggcgctgg gccgaagagg ttacttagtg
     7561 cagttcgcgc actgtttgtt gacgatctgc tggaagaagt cgttaccttt gtcatcgacc
     7621 aggataaacg ccgggaaatc ttctacttcg attttccaga tagcttccat acccagctcc
     7681 ggataagcga cgcattccag atgcttgatg ctctgctgcg ccagtaccgc cgccggaccg
     7741 ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg cgtcggtaac ctgctgactg
     7801 cggttacctt tcgccagcat gatcatgctg ccgccgtggg attgcagcag atccacgtag
     7861 gagtccatac ggcctgcggt ggttgggcca agtgaacctg atggataacc ggcaggggtt
     7921 ttcgccggac ccgcgtagta gatcgggtga tctttgatgt actgcggaag ttctttaccg
     7981 gcgtcaatca gctctttcag cttggcgtgt gcaatatctc ggcccacgat aatggtgccg
     8041 gtgagcgaca aacgagtgga taccgggtat tgcgaaagct gggcgaggat ctctttcatc
     8101 gggcggttaa ggtcaacttt caccgcttcg ccttcaccgg cctggcgcag ttcttgtgga
     8161 atgtactggc ctgggttgtg ttccagtttt tcgatccaga taccttcgcg gttgattttc
     8221 gctttaatgt tacggtcagc ggagcaggag acgcccatgc cgaccgggca ggatgcgccg
     8281 tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt atttaccgcc aaactgcgcg
     8341 ccaagaccga gtttctgggc ctcttccagc agttcctgtt ccagctggac atcgcggaac
     8401 gcctgaccat gttcgttccc ttccgtcggc agttcatcgt aatagtgagc gcttgctaac
     8461 ttgacggttt tcaggttggt ttccgcagac gtaccgccaa tcacaaacgc gatatggtac
     8521 ggcgggcagg ctgcagtacc gagggtacgc attttctcga cgaggaagtt tttcagtttg
     8581 ccgggagtca gcagggcttt ggtttcctgg tagagatacg ttttgttggc agagccgccg
     8641 cctttcgcaa cgcaaaggaa tttgtactca tcgccatcta ccgcgtacag gtcgatttgc
     8701 gcaggcaggt tagtgccggt gttgacctct ttgtacatgt ccagcgccgc attctgtgaa
     8761 tagcgcaggt tatcttcgat ataggtgtta tagacgcctt tcgacagcgc ttcttcatca
     8821 ccgccgccgg tccacacgcg ctggcttttt ttaccgacga tgatcgcggt gccggtatcc
     8881 tggcaggtcg gcagcacgcc tttggcggcg atttcggagt ttcttaagaa ttgcagcgcc
     8941 acgtacttgt cgttttcgct ggcttctgga tcgtgaagaa tagccgcaac ctgtttctgg
     9001 tgtgccgggc ggagcataaa agaagcgtcg tgaaaggctt gctgcgccag cagggtcagg
     9061 gcttctggtt ccactttcag gatggtttcg ccgtcgaagt cggcaacgct aacgtaatcg
     9121 gaagtgagta gatagtattc ggtattgtct ttccccatcg ggaaaggtgc ctggtagata
     9181 aagggtttgt ttgacatagc ttccagcctg taactctgta tttgttattc gaaaatggcg
     9241 tgccgcgtag tcacgggcgg cacgcaaagt gcatttataa gaacccgtac atcgcggcga
     9301 agatccagcc gaagacgcac gatacgctca caccaatcaa ccccggcaga ataaagctgt
     9361 ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg gtcaaactga atcgctgcca
     9421 gatcgctcgg ataagtcggc aggatgtaat aaccgtagca agccggtgct gaagccacga
     9481 tgtatgccgg atcaacgccg atcgccagcg cgaccggaac aatcgccgcc agcgccgcag
     9541 cctgagagtt tacaaacttg gaaaccagca gcagaacaat ggcataggcc cacggatact
     9601 ctttcaccat ttcacccagt acgccctgaa tttcagacat atgcgcaccg aacatggttt
     9661 ctgccatcca tgcgataccg tacaccgcca cgatggcgat cataccggaa cggaagactt
     9721 cgttttttga gatagacgcg ggattggttt tggtcaggat aataatcagc gccccggtca
     9781 gcagcataaa catctgaata accagtacca tcgacagcgg tttgccgccg aaggatggac
     9841 gcaggtccga atcagcacca agaagggcga ctacagcgat tgcaccgagg aaaatccaca
     9901 ttgccagcca gttgcttttc ggcagttttt tatcgagcag cgtcgcggta tcaccgtaaa
     9961 catactcacg gttttccggt acggagatga atttctggaa ctcttcgtct ttatccagat
    10021 ctttaccgcg gaaccagctg aagataccga tcgccaggat accgattaac gtcgatggaa
    10081 tggtgattgc cagcaggtcg aggaactcaa gatggcgacc atcaaaggtg acattaccca
    10141 gcattgcaac cagagacacg accgcaaccg acaccggact ggcgataatc cccatctgtg
    10201 caccgataga acttgccgcc atcggacgtt ccggacggat gttgttctta atggcgacgt
    10261 cgtagatgat cggcagaatg gtgtaaacca catgacccgt accgcaaaga atggtcagtg
    10321 tacaggtcac aaacggcgcg acaattgaga catatttcgg gttgcggcgc agcaacttct
    10381 cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg caaggtcgcc gatgccgcca
    10441 ccaccgcaat gataaccagc atgacatcaa ctggtggttt acctggctga aggtggaaga
    10501 cgaagaccag aatgaccaga ccgataccgc ctaataaacc cagcgcgata ccaccctttc
    10561 tggcaccata aaacagacat atcagtatta tgataagttg gatagtaaat aacatgtgtg
    10621 aaccctcgcg ataatcctat ttaaattttt gctgaataga tcacagtcac gttctgtttt
    10681 gtatgaactg ttttcagagc ctgactaaat atccgtttgg tcgttaccgg ctttagcaaa
    10741 tacctcacag taaatattgg ctgattgaag cagcggcaaa tttatgcact gtgataagcg
    10801 gctttttcag agagaaaaac tctctggcga tgggtttata gtttgtactg cgcagatgtt
    10861 aatgttttgt ttgctgttat cttattgata tttatcggtc tattttttgt tctcagggca
    10921 gatatcatta ggtttaaaga ttaatctgat ctacccattt gtgggtaaaa atacacataa
    10981 tgcgggtgac ataatagtta attaactttt gttagcgttt tgaaattaaa aacaccgttc
    11041 acctgaagag atattaattt ttagcgatga tggagggata attatatttg atctggcaca
    11101 agttttactg atgaaggatg taacttgtgc caggggtatt ttgcattacg gtaataatta
    11161 ttacttacag ataaccagcg atccgttatt ggcaatattg tttcagtagt gagtagtgtt
    11221 ctgcctgaat acggtaacgg taaaccggac gccccgtgac gccataatgg atactggtga
    11281 acaagatgtg gcagttgacc agccagatga ggtatttacg gcaggaaaca cgcgagatgt
    11341 taacctcgtt agccagctcg tcggttgaaa actcatagtc ctgatgcgcg tcaatccact
    11401 ggcacagcgt gcgtaacgtc tgcggcgtta agccttttgg caagcgacga ggatcctgtt
    11461 cgttggagct gctgccgtgg attagctgat caagctcggc ctggtcataa tactgatgtt
    11521 tttccagcgc cattttcttt tgccgccagc cggtgagcgc ctcttcaaag cgggaagcct
    11581 ggaagggttt gatcaggtaa tccacgacac cgtaatgcag cgaatcttta atggttgccg
    11641 catcggctgc ggaggagatg acaatcacat cacttttgca acgcgcgtta tgcaggacag
    11701 gcagtaaatc gagcccgttc tctttttgca tatagatatc gagcaatatc aggtcgatag
    11761 gcgtatcgct attgaagata atctctttgg ctttctccag cgtcgaggct gttccacagc
    11821 attgaaagcc tgggatttgt gctacgtatc ggcgattcag ctccgcgacc attgcgtcgt
    11881 catcgataat taatacattg atcatctgtt cgacctctcc ccgtcccagg gtatctggac
    11941 aaaaaattgt gtgaaaatcc cgggttccga ttccacggcg atgctgccgc cgagattttc
    12001 tacctgttgt ttgacaagtg ctaaaccgac gcctcgctcg cttccttttg tcgagacacc
    12061 tttgtcaaaa atgtgatcga ttttgtcggg tgcgatcccc ggtccatcat cgttaacttc
    12121 gcagtgcagc cagccgtgac ggtagtgcaa tgttacgcta atttcgccac cgggttccgg
    12181 ccctaatgct tccagcgcgt tttctatcag atttcccaat gtggtaatca gcgtcgcgac
    12241 ctggtcctca ctgccgctgt ctggcagctg gctttcactg tttaaaatca gcgtatggcc
    12301 taaatcggtc gcgcgattaa tcttgctgat taaaaaacca gcgataaccg gagatttgat
    12361 cttacccagc agagagccaa tctcttcctg atagttattg gctgttttga gaatgtaatc
    12421 ttccaactgc ttataactct tcagatgcaa taatccgaga atcacatgca atttattcat
    12481 aaattcgtgg gatcgttcac gaagtgcatc agcatagttg accagaccgt cgagtcgctg
    12541 catcagttta cgaacttcag ttttgtccct gaaggttgaa atggcaccga tgataacgcc
    12601 attactgcgc accggaacgg tgttgatcag taatagccgg tctttaatcg taatctcttc
    12661 gtcgcggcgc ggcgtaccgt cgcgtaacac ttccgagaca tctaccacct gtgaccatga
    12721 gtggctgagc gtcgacagtt tctcatcgtc ctgcgactta cggtaattca gcaattcttg
    12781 tgcggcatcg ttgatcagcg tgacctcgcc gcgatcgtcc acggcaacga cgccttcttt
    12841 gatagactgc aacatggcct ggcgctgctc aaacagcgtg gagatttcgt agggttccag
    12901 gccgaaaagg atttttttca gtaccttaac cagaatgcag gtgccaatca gtccgaccag
    12961 catgccaaat aataccgacc agataatgct ccagcgactg tcattgattt gttgggtcac
    13021 gcggcttaac tcaaggccga tcgccaccac gccaatttgt ttatgatttt catcgtagat
    13081 gggggtaaat acgcgtaaag cctgcgccag aaaaccgcga ttgatagcga cattttcttt
    13141 gccattcagc gctttaagga tgtcatcacc tttaaatggc tgaccaatac gctgggcttc
    13201 aggatgcgag tagcgaagac tttgcatatc ggtaacgaca ataaacagca gatcgttgcg
    13261 tttgcgcacg gcttccgcga tggcctggat tccgctctcc tgcggttttt tctgcaagcc
    13321 ctgacggatt tccggcgagt cggcgagggt acgcgccact gccagtgcct tgttggccag
    13381 cccgtctcgc gtcatatcac tgatttgcga gaagtaaatc agatgcacca ccaatagcac
    13441 cgagaacagt accgcgctga ccattaagat cactgtggta ctcaatttca tcggacgttt
    13501 gcgtaacatg cggtagggca atgaatgtct catcagcttc cttgtgtgac aaatttctta
    13561 agcattatct ctgatgaggc gggtaattca aagggagtaa gaatgattgg ctatataggg
    13621 gaagagactc tggcaacgga aactgccagt gctgtatgaa gattccaggg ctatgcttat
    13681 agcgataatc atactgatga gagagggaaa gtcatggatc aggcgctact ggacgagggt
    13741 tatcgctgtt ataccggcga aaagatcgat gtctatttca acactgcgat atgtcagcat
    13801 tctggcaatt gcgtacgtgg caacggcaag ttatttaatc tcaaacgaaa gccgtggatc
    13861 atgccggatg aagtcgacgt cgtcactgtg gttaaagtga ttgatacgtg cccgagcggc
    13921 gcgctgaaat accgtcataa ataagcgagg gtaaaatgga aatacgcgaa ggccacaata
    13981 aattttacat taatgatgaa cagggcaagc aaatcgctga aattgtcttt gtgccgaccg
    14041 gagagaattt agcgattatc gaacataccg atgtcgatga aagcctgaaa gggcaaggga
    14101 ttggtaaaca gctggttgcg aaagtcgtgg aaaaaatgcg tcgggaaaaa cgaaaaatta
    14161 tcccattatg tccatttgcg aaacacgaat ttgataaaac gcgggagtat gatgatattc
    14221 gcagttgatg ggagagtaca gattcacgat atttttcatg ctctctgcgg tgtgatgcag
    14281 gagagcatct gaagggtagg gggatgcaca aagaatgggc agagagcgcg tttttttgtc
    14341 ccaagtcatc ccctttactg agcaaaaaaa agaatatctc ctatatgaga atcatcaatc
    14401 ggggttaata agttttgcgt ccccagagtg ttcaatattg ataggagtca tattatggaa
    14461 ggtaaaaaca agttcaatac ttatgttgtt tcttttaatt atccatcatc ttattcctca
    14521 gtgttcttaa ggttaagatc attgatgtat gatatgaatt tctcctctat cgtggctgat
    14581 gaatatggga taccacgaca attgaatgaa aactccttcg caataacgac atcgttagcc
    14641 gcaagtgaaa tcgaagattt aatcaggctt aaatgcttag acttaccgga tattgacttt
    14701 gacctcaaca ttatgacagt tgatgactat ttccgtcagt tttacaagta gccaggaagg
    14761 ataagaagaa cataaaaaat ggcactattc tctaaaatat taatttttta tgtgattggt
    14821 gtgaacatat cctttgtcat tatctggttt atctcacatg agaaaacaca tattcgttta
    14881 cttagtgcat tcctggtcgg aataacctgg ccaatgagtc tgcctgtggc attacttttt
    14941 tctctctttt aggagctgta gttgagtgga cccgctggtt atcgtgaaac gaagcgtctc
    15001 tcctcaaaac tggatttgcg gaacgatgaa cgaagcagtc gcctgactgc ttcgttcatt
    15061 aaagtgaaat ttatttctgt gggcgcatcg ccgggaagag aataacgtca cgaatagtat
    15121 gactgtttgt aaacagcata atcattcggt cgatgccaat acccagacca gcggttggcg
    15181 gcagaccata ttccagcgca gtcacgtaat cttcgtcata gaacatggct tcgtcgtcac
    15241 ctgcagcttt agcgttaacc tgttcctgga aacgttcagc ctgatcttct gcgtcgttta
    15301 attcgctaaa accattaccg atttcacgac caccgatgaa gaattcaaaa cggtcggtga
    15361 tttccgggtt aacatcatta cggcgcgcca gcggggacac ttctgccgga tattccgtaa
    15421 taaaggttgg ctgaatcaga tgtgcttctg ccacttcatc aaagatctcc gtgacaatac
    15481 gtcccagccc ccagcttttc tctaccgtaa ttccgataga ttcagctaat gctttagcag
    15541 catcaaaatt atccaggtcg gccatatcgg tttctggacg atattttttg attgcttcgc
    15601 gcatggtgag tttttcaaac ggtttgccga aatcaaacac atgctcgcca taagtgactt
    15661 tagtggtacc cagaacctct tgtgccaggg tgcggaacag tgactctgtc agttcaatca
    15721 aatcgtggta atccgcatac gccatgtaga gttccatcat tgtgaactca ggattatggc
    15781 gaacagaaat accttcatta cggaagttac ggttgatttc gaatacccgt tcaaaaccgc
    15841 ctacaaccag acgtttcaga tacagctccg gcgcgatacg cagatacata tctaaatcca
    15901 gagcattatg atgggtaata aacgggcgag cagatgcccc acctggaatt acctgcatca
    15961 tcggggtttc tacttccata aagccgcgcg cgaccatgaa ctggcggata gcggccagaa
    16021 tttttgaacg gacaacaaac gtttgacggg atttatcgtt agcgatgagg tccagataac
    16081 gttgacgata acggacttcc tgatcctgca gaccatggaa tttatctggt aaaggacgta
    16141 gtgcttttgt cagcaggcgc aactcagtac agtgaatgga aagctcaccg gtttgcgtct
    16201 taaacagcgt accgcgggca ccgataatgt cacccagatc ccatttttta aactgatcgt
    16261 tataaacacc ttctggcagg ctatctcttg caacgtacag ttgaatacgg ccaccgacat
    16321 cctgcaacgt tacaaaggag gctttcccca tgatacgacg ggtcatcatt cggccagcaa
    16381 ccgagacttc aatgtttaag gattccagtt cctggttatc cttcgcatca aactcttcgt
    16441 gcagctggtc agaggtatgg tcgcggcgaa aatcattggg aaacgccaca ccttgctgac
    16501 gtagtgccgc cagtttttcg cggcgatttc tcagttcatc gttaaaatca atagcctcat
    16561 tggctccccg tgtttcttgt tcagacattt tggttcctct aaatccagct ttcaatttag
    16621 cgtagataaa gagacagatc ggtacactgt attatctgcc tcgactatta ataactcagt
    16681 ttatcttatc gaaactaacg cttacctcaa gttgatatca ctaataaata accatcaaaa
    16741 tggtttaaat gacttattaa tctagatttg ctagtgttat aaactgaaag ttaatttatt
    16801 cataaccaat tgtttttacg acagttaatc gttgctctcc tgtatcatat tcgtcggcgt
    16861 gctaaacaga aaacgggtag caaaggccaa tacgacgatg atcgcgacac aagccaacgt
    16921 ccactctccc atctgagaaa agaatcgctg gtaagctgca attgccatac cgctaatttg
    16981 cgactctgtc gtctgctgtg ccacgacgcc tgccagccag ttggcgaccg cgcctgttgc
    17041 cagcatataa ataccggtta atacgccaga cattttcaga cgcgtgattt gcgcaatcgc
    17101 caccgggtca ataaagagtt cggcaaagcc cattagcgcc aacccggata tcatcacgcc
    17161 cattgacgct tgaccgtcag ccgctgcatg tcgggcatca aatgccaaca acataaagcc
    17221 acaagccatc agcagtaagc caaaggcaaa cttcagccag acgcgcaatg ttgagttgcc
    17281 gcggctttct ggcgacgcca gccaggccag tacaacccca gcgagcatca ccgcaattgc
    17341 attcaccgac tggaatagtg ctgtaggtac ttcaatattg aatgcctgac gattcacaaa
    17401 gcgatcgata aacaagctga tggtactgcc gccctgttgt gccagtaccc agaacaatgt
    17461 cccgacaaac atcaacaata caatttgcca aagagcacgg cggtgttcgg ggaatttgac
    17521 catcatgcgg gcaatgattt gtgcggcaat gaggcaaacg atcgccagca aatatccgga
    17581 ccagtcgttc tccagcagca gggtaaaaaa tactggggct aaacagagca tcaccactaa
    17641 ccagctccat accggtaagg caaatttgac gctagtgagc gcttttttat ccatactacg
    17701 cgtggactgg aaatgacgat gcccgcttaa gaaaatcaac aaaccgataa acatgccgcc
    17761 acccgcaagg gcaaagccaa catgccatcc ataccactga gcagccaggc cgcaggcgat
    17821 tggggcggcg atagaaccga tattgcccgc agcatacagc agcgaaaaac cgccatcacg
    17881 tcgatgatcg ttctcgtcgt aaagctcgcc aagcaaacag ctgatgtttg atttgaataa
    17941 accgtagcca caaataatga ttgccagcgc cagatacagg ctaaaggttg aatttgtatc
    18001 aatgcccagc accacatggc caagggtcat taacagcgcg ccggcaatca ctgcagtgcg
    18061 gttgccgagc aggcggtcgg caagccagcc gccgagaata ggggtaacgt aaaccagaga
    18121 agcatatgcg ctgaacaggc tgatggcatg gttatcatca aaaccaagct gatgggtgag
    18181 atagagaatg agtaaggcac gcatgccgta aaaactgaag tactcccaga tttggatcgc
    18241 cacgatatag tatatcgcgc gcggctgtga gggtgttttc atgtgttctc cttatgagca
    18301 aaaaagggaa gtggcaagcc acttcccttg tacgagctaa ttattttttg ctttcttctt
    18361 tcaatacctt aacggtatag cggccatcag cctgacggta tgcaccgtga atatcggttt
    18421 caaagcccgg atagtgagcg ccgatttcac acagcatctg caggaactcc agaaccggac
    18481 ggctttcttc ggtgatcatt tcacccggca ttaccagagg aactcccggc ggatatggaa
    18541 ggatcatatt ggcgttaata cgacctacca tttcgtcgag gtaaacttct tcggtcatac
    18601 cgtgcagctc tttctggaac gcagcatacg gagtcattac catcgtcggc agcacttcaa
    18661 atgcgcgata catcagatcc ggcagattgt ggtgaacaat cagtttgtgg atattctgag
    18721 ccagttcctg aatacgcatg ttttcataga attcaggatc ttcacgatac agagacggca
    18781 gcatgttttt cacacgcagg ttcaggtcga acgcacgttt gaagtcagtc agagcacgca
    18841 gcaggctcag tgctttggtc ttatcgatac cgatgctgaa caggaacagc aggttatacg
    18901 gaccggtttt ctcaacaacg atgccatgtt cgtcgaggta tttcgccacg atgctggccg
    18961 gaataccaaa gtcgctcatg gtgccgtctt tttccatccc cggagtcagc agggtgactt
    19021 tgatcgggtc aagatacatg tgctcgttat cgatgttttt gaagccgtgc caggtgctgt
    19081 cagaacgcag cggccagcat tcagtggtat cgatatgatc cggctgccat acatcaaaga
    19141 accagccatc agattccgtt ctcagacgtt tgatctcttt acggaatttg atcgcacgtt
    19201 caatggaacc gttgatcaga cgcttaccag cattgccttt catcatcgcc gctgcggttt
    19261 cagtggacgc cacgataccg tagtgcggag aagtggtggt gtgcatcatg taggcttcgt
    19321 taaaggtttc ttcgtttacg tcacctttaa cgtggatcat ggaagcctga gagaacgccg
    19381 ccagcagttt gtgagtggac tgggtttcgt aaatcacttt cccttctaca cggccaccgc
    19441 tcataccgca tttaccttcg taaatcggtg agaagttggt gtaaggcacc cacgcggagt
    19501 caaagtggat ggatttcaca tccagtgttt tcttgataaa gtcggtgttg tacagcagac
    19561 catcataggt agagttggta attacagcat gtaccggcca ggttgcgttt ggtgtttctt
    19621 tcacgcgctt agcaatggta gcgtgctgga attcactctg tgggatacca ccaagaatac
    19681 cgtaagcgtt acgggtcggg cggaaataga ttggcgtaac atcgctcatc atcatcaggt
    19741 gggtcagcga tttgtggcag ttacggtcaa tcagaatggt gctgcctgct ggagcagagt
    19801 acataccaac aattttgttc gcagtggaag taccgttggt caccatgtag ctgcggtctg
    19861 cgttaaagac gcgagcgata tactgttctg cttctttgtg tggaccactg tgatccagca
    19921 gagaacccag ttcagatact gaaatggaaa tatcagattt catggtgttc ggaccaaaga
    19981 aatcatagaa caggctacct accgggcttt tctggaatgc agtaccgccc atgtgaccag
    20041 gagtacagaa agtatattta ccttcacgaa catatttaaa cagtgcttta gtcagcggag
    20101 gcagaatagt gttgatatat tcgtcagtgg tctgcttgat cttattagca atatcttcag
    20161 cagcacccag cgcatattca aagaagctaa tctgtaaacg caggtcattc aggcttacat
    20221 cgagagtgga atacgtatta gcgaacgcgt acaacggcag gttctcgttc attttgctaa
    20281 tttcttcgca cagctcgaga ttatatttat cccagtcaaa aataacgccg cacagacgcg
    20341 cattgttttc gatcagtttt aataagtcgt cacggtcgtt cgggtaaaca atctggaagt
    20401 tcagacgttc aagcgcgcga tgaagttcac ggatgggttc ttctttaaaa taaaccccca
    20461 tgtgattcaa tattgcaata acgttcatag tcatatctcc aggtaaaaaa ggcccctccc
    20521 aacacatggg acaaaatgaa aggaggagcc tcggaaaata cttttaatta atgtgcgtta
    20581 gacgcggtgt ggttatccat tgagtggctc tggcgctcgt gcattttgcg agcgtagaac
    20641 atcaggataa tcaggctgac gatgaaggta cctgccagct cgaaggagct tgcgcccatc
    20701 agcgcgatga agcagaacac gcaacccagt acagagcaga tcaggctgac aaagttgcgg
    20761 atgttaacgc cttcaaaacg aatcaggtca acgcaagagt agaagtacgg cagcatagtc
    20821 agcagtactg cgataccggt cagttcaccg aacaggtcag atgctttacc accggcagag
    20881 ttcatcagag tgatgaggat catcagggca gtcattttca ctgcagccag cagcagacct
    20941 tttttcggaa taccgttgct gtcgacttca ccataaactt tcgggaagtt accgtcgtta
    21001 gcggcacgta cacctgcctg gcctaccaac atcatccagg agcccagaga agtcaggcac
    21061 gcaaaggcgg tgaatgcaga aaccagcggc gcagcccagt taccgaggat agttgaagca
    21121 ctgattgcaa acggagcacc ggaagccgcc attacagaag acggatacat accggaaagc
    21181 acctgagtcg cagcgatata aacaatacct gctaaaccag tacccagcat ggttgccagc
    21241 ggaacggtac gtttcgggtt tttaaccata ccagtactta ctgctgcgga ttcaacaccc
    21301 acgaaggccc acaggcagag cagaatactt ttaatgatcg catgaccatc agtggtatcc
    21361 gcagtattcc agttagctgc ataagttgcc gcatcaaacc aatgccagcc aacaatagca
    21421 gtcatcacca caggaataag aaccagcacc agaccaatag tggttaaacg gcttacccag
    21481 gtaccgccga gcatatttac aaaggtaaat acccagacga tagcaataca ggcgataccc
    21541 gccggaacag gatcatttaa tactgggaag aaggtggaaa gataagatac agcggtaata
    21601 ccaatcgcca ggttaccaat ccagttagca tggtaataaa gaacacctgt ctgaaaacca
    21661 aatgcagggg aaatttctcc ggcataagca attgggccac cttgttgcgg gttttttgtt
    21721 gccagtcggg catatacata cgccagcgac attgcaccaa taatagagat aatccaaccc
    21781 cagatagcaa taccaccgat acttgctagg ttcgcaggta ataatgcaat accgctcccc
    21841 atcatattac cggcaacaac accggtacag gcaaatagcc cgatcttctt ggcagaactc
    21901 atgctcttct cctaatttca tttttgaatt tggagtccgg gtcatgatgt ataactattt
    21961 cctgaccaga ccaaactggc gctaagaata ctcacgaaaa aaggattaat cctaaagatt
    22021 aggtgaaata acacaaaagt ttctgtaagt gagaacttga ggttttttat taacacatca
    22081 ggatcgcaag ttgatatcat gaaaagataa acattcaatg tttacaatgg attgcgggat
    22141 attctctggt taaatttatg taataaaaat tatgcggcaa ataaattgcc gcaacatatt
    22201 ataccaacag aaacatacaa aaactcaaca acaaatattc ccgagcataa atcaacccgg
    22261 gagttactta ttctgaagcg agaaatttgt cgagataagg tacaacataa ggaacagaag
    22321 tctggaatat accattttca atccagtaaa gggtgtttgc ccctgggcgt aaattaaagg
    22381 cggtgagata tgcatcagcc gcttcccggt tcatcccctt catttcataa accttgccaa
    22441 gcaacacata atttagccag gacatttcaa gatcaatgcc agtatttatc gcctggtaag
    22501 actcatctgt tttacctttt accagagcac tgaccgcttt tatttgatat ataatggata
    22561 ggttattcaa ttccggtagt gtaacaatgt tgtctatttc tgtgttcagt gctgctaatt
    22621 gtttttcatc taaagggtgt tgagaatggc gcacgatatc aactaatgct ttttctgctc
    22681 tcgcgtaggt aaattctggg gatgattgaa caatctcacc taataattca ctggcacggt
    22741 tcaatgattt atcatcgcca tgcagtaaat aatcatgtgc ctgataaaaa ttagttaata
    22801 acgcaccacg atgaggcaaa atttgctgga gcgtctcctg cattcgttgt ggccacggtt
    22861 ggtttaatgc ttttgataaa ctctccagta aatcattttg aatcgccagc tgattaccgt
    22921 tagtgatgac ataacgttta tccagcatgg ttgaaccatc tgcattgtct accaatttta
    22981 tcgacataaa gcattgttga gcacggtatt ggcgctgatt aacaaaggca atagataatg
    23041 ttttaccgga actgctcggt tcatcaatgt tgtagttgat tttgtcatgc accataaagg
    23101 tggagaaggt gttaagtgat gtcgccacca aatcacccac gcctatcgcg taagagagct
    23161 gatacgggga actccagctg ttacaacttt tatttaccat attaatgtca atatcgcgtg
    23221 gattgagcaa aatacgcgat ttgctcatag gaagacgtgt atcaagagtt gaaaacgcta
    23281 ccagtgctac acagatacct aacgacaaca ggaaaaaaaa ccatacccaa aaggtagtga
    23341 atcgtttgct tttaactggg gattgttcag gtggcgttgc ggtgttttga atgttaagac
    23401 tgtgggaggg agaatctgtg gcaggaaccg cctctggtat agggggaggc gaagatagca
    23461 ttatttcctc tccctcttct tcgctgtacc agataaccgg caccattaat ttatagccgc
    23521 gctttggtac agtagcgata tagacaggac tatcttcatc attatctttt aatgacttac
    23581 gtagttctga gatactctgc gtcacaacat gattggtgac aatacttctc ttccagacat
    23641 tatcgataag ttcatccctg ctaagtactt cgccactgtg ttgagcaaag aaaaccagaa
    23701 gatcgattaa tctcggctca agggtaagtt gacgcccatt gcggctaatt tggtttatgg
    23761 acggagtaac aagccattcg ccaacgcgaa ctacaggttg ttgcataata gaaactcatt
    23821 cgaaaaggga atgatgcaat gataattgcc acaacctatt tttaccatct atagatgggt
    23881 ttatttacat attattggtg aatgtaagac gttattttta ccagccataa acttcttgat
    23941 tacatagtat tacgaaagga ttttactgag aaccagaagt aatattcctt accatcaaaa
    24001 ttcatcatct ttgccaaaga aaaatgttca gaaaataatc catggaaaat tgtccggagc
    24061 gcttactatt ttaatggatt gttagtcttt gcatgagcaa gcggactgat acatttctct
    24121 ttgttctcat tcagaaaatc tcatcagtcg gcgttcagac cggtgttgtg ctttatggat
    24181 gcgttacgct cctgatgacg tcatttgacg ttcaacaaca tcacggggcc gcacgacatt
    24241 tcacgtcagt tagtgctatc gctcaggaac aaattttccc gaattgggat atgcccgcaa
    24301 attgctggtg atgtggggag aatctggttg agttcgggag aattgatttg gaggcagaac
    24361 gcttaaattg tggcgtccag aaacgaaaaa cggacctccg tggaggtccg ttgatatgaa
    24421 tttggtgccg gactcggaat cgaagattgc ttctatcatg ttgagattgg ttaattttgt
    24481 ttggtttggt taaaaatgta ccctcttttg taccctcagt cccttttgct gtgaaaaagg
    24541 aatcaaaccc gggagttggg agatgaggtg aaaggggggg acgcatatcc tggtccattt
    24601 cagtatgtgt tatctctggt ttttgccgtt tccgctgttg tatacccact cagcaataaa
    24661 ctgttcagcg ggtagtggag gtgcatagcg gtatccctgc agatagcagc ttcctttctc
    24721 tgtcatgaac aattcctgtg attcggtttc aatgacttcg gcaatgatat caatacctaa
    24781 ccggcgagcc agctcaataa cattttctac tatatgcccg gagatttcat ccacgccgac
    24841 catttgtaca aaccctttat cgagtttgat gtaatcgaca ggaaacgact gcaggtagct
    24901 gtgggttgca tagccagtac caaaatcatc cagtgcaaac aatactccct gttgatgtaa
    24961 tttcgcaaat aacgcttttg attctgcggt gacggatagc tggtttcgtt ctgtcagttc
    25021 cagaaccagt ttcactttcc ctttcgggaa ggtggataga aacttcatac agctatcctc
    25081 cagtcccgga gacagaaaat gcgctggtga aatattgatc ccaatatgaa attgattcgg
    25141 taaaaaatgc gcaacagaat taaggctatg ttcgacctga gacatcaact gttgcgttat
    25201 cggggcaatt aatccggtgg actctgccag cgggataaac tgatctggtg ggataattcc
    25261 gctgacgggg tgatgccagc gaatcagtac ttcgcagccg gttagctggt gatttttagc
    25321 ggaaatgatg ggctgaaaat aaggtctgaa ctctccgtga gcaagtgcat ttttcagcat
    25381 atcgagcggg gaagatggac gtcggcggga cagcagaaag gctgtgagta ttccaccgaa
    25441 gatggcaaca gggatcatat accaggcctg tttccaggcg taggtaagtt gcattttcac
    25501 cgggatgata taaatcaggg agaacggata tttttttgaa tgactttctt tttgtatacc
    25561 gctgtcgtcc gggagcagag tgacattttt tccgctggct gacaggaccg tgtttcccaa
    25621 acggaatcgt attgcaggaa ccgcatgaac cacagagatg atgtcctgaa taaaataata
    25681 atccgtggcg gtaatgaccg tgccgttttt gtaaggtgtt cttaacagga tccagggcag
    25741 agtacctgta tcttcaggaa aacgaatcag catatcgtgc cgccactgtt ccttttcagc
    25801 aactggaccg acaggtaaac cggggtgggt gctacaaaat acagcctctt gtcttgccag
    25861 gctcagtgca cgaagttgtg ggcgtttcag cagcagattg cttaattcat gctgagtatc
    25921 tgcgttacag ttattcagaa gcaaatgttc tgcttcctgg ctgatgctgt tgttctgatt
    25981 gagaatgttt tctatatagc caagtgcgaa attaactctg atctggctgt cattcaccag
    26041 ctctgctttt ttccagaaaa atgtcagcag aataatgaac agaaatatca gactacctgc
    26101 caccatagct ggagtatgtt tttttacaaa taatctggaa aacatgtttt tttgctctga
    26161 atgacgccag atttatcaga atgtttttgt gtatgtactg agtctgtgct ttgggttaaa
    26221 cgggcgataa ggtttccgga tagtatttcg caataatatc attcagtctg tcaaccagct
    26281 ctggtttcct gaacgtgatg tgcgcggagc ccttcttaaa gtatctgatg ctgaacatct
    26341 catcttcata gcacgcttta ccctgaactg agtggatgtg atcatccaga tgaatggtta
    26401 tgttttcccg gttgtcagga atcggtttgc cgctgaacaa atacagcatt ctttccaggt
    26461 cggcaagtcg atctgcctgt tgcccggtga taagatgaaa tccccaccgg tcccatctca
    26521 ccagattgtt gacgataatt ttactgccaa atttacaggg gcaattagtt ttgtaattcc
    26581 agttcagtcc cctgaagacg ttgatcaccc ctcgctcaaa cacttcatct atgttctggt
    26641 gcagttgttc aaatgtgctc agaatgttcg cttcactgat ttccggaaaa ttatcatatt
    26701 ccagtgatcg gtaccatgta tcacgggcct gtgcatccat taaggaaagc atccctgaac
    26761 gttgcatcag gtcacgccag atttctcgat cgagattgcg ggtgatggct ttcattgctg
    26821 cttccggttt ttccatcagc cagcagccac agcgaaaatt ctgtttcatc gcccagtcca
    26881 gggcggtttt gccgccaatg cctgctgtca gcactgagat ttctgccagt tgccggataa
    26941 gcgtttcggt ttgttgcagt gctgcattcc gtaccgtcac aacgtgtcgg atactggtgg
    27001 agcagataac gtccgtatgc ccggtgacca cttccggacg ggagattgtg atgtctgaca
    27061 ttatcggttt tccttttggt ttatagacga aaacgccagc tgtattcagg ctggcgtcag
    27121 gggaatgaag tatggtgagt gatattcacc ggttctggtg cagaagctgc agatgacgaa
    27181 cagcacggac atcctcccgg gcctgttcga tgcagggcaa cagtgctgct gaatccgtct
    27241 tatcctcatc gttgtcgaag ataatcccgg attcacagtc gatattgtcc tgcagccacg
    27301 caatcagaat atccagtgct gtttccgtgg ttaatgattt catgttgttc ctggttacca
    27361 gttgaaagtg ggtaaacccg gcaggcattc agcgctggca tccagatgaa tgagactgac
    27421 gccataacgt cgggtaagtg tgacaaccag acaacggaac gtttttgaca ggcccaggcg
    27481 cttaagccgc agaaccgggt atgaccacgc atcggtacgt aacagatagc cgctgcctgt
    27541 aaaatgaatc cattctgatt cgccgtcgtc attagtctgg tgtgacagcg agtacagcca
    27601 ggcgttgtcc gtttccgtga tatgtgcggt gctgcaacgt atgcaggtaa gggttgttaa
    27661 cggtggtggt gtactggttg cgtgttggtc ggatttatcg accacgcgga gtaaataacc
    27721 gttttcagag accttgttaa tcagttcact gagattaaca ccatcgacgt caacgacaat
    27781 gcgccccatg ttcagtgcct gcacgttaac gctgtcggct tccagcgtca gggccagttt
    27841 cattgttttg cctccggatg cttgccctgg gtaatgttat ttaccattct gtaattgttc
    27901 cgggtcatca ggccggttgc cctgcgagcc cggagaatat caatgctgtt gattaactgc
    27961 gacggggctc ctgcgctgaa tcccggctgg tcggtacgta ccagcgcgta tttttcaacg
    28021 agaaagttca ccgcgtcaca cagtgaaatc cctgcctcaa tatgctgctc aatcacacgt
    28081 tcatcagcga acggtgtgtc attcagcgtg aggccatagt gctggtccag cagtcgggtg
    28141 agcagtgtct gccagatggt gacgggagac gggcagcccg atgcctcccg tacgtgagtg
    28201 tcgggtaatg tattcatctt aagttctctg taacaggtgg tgataaaggg gagatatccg
    28261 gggttatacg gtgattgcgg gcgctgccgg tgtcggataa acagccagat aaacgtaacc
    28321 acaactgcca agggtatcgg cttcgcaggt cagccctttc gcatacagtg tgatggtatg
    28381 ctgatggcgg ggattcagtt caccgctggt gagcatgagt tccagttgtt tcatcagcag
    28441 cggaaaggcc tggtccaggt ggtatgcatc cgcgtcgctg aaccggcctc tgataccggc
    28501 acggtcggca aggtaatgca accggttacc ctcctgcacc agacgtgccc cgaaacaggg
    28561 cgtcacggtg cagggcagcc cccaccaggg gcggtcgtga ttgtcgtcgg gatgcgttgt
    28621 cccgggcagt gtgtctgaca cgataaagtc ctcacagaaa tcagttaaga atggttcgat
    28681 ggtggcgata attcggctca tcagaatgcc cttcctgact cagttcaggg tgacgctcat
    28741 cagccggaca tacgggccaa aactgtcttt acggcgttct gcaaacacag ccagcacacc
    28801 gggaatatcc tgcacttcac gaccggtata cgcttcagtg ctgccgtgcc agcggtattt
    28861 gccggtacag aacggaaaaa gacgggatgc cggatgttgt tggtgaatac gcatggcttc
    28921 accacgggtg atgattttca tagtgtgata cctctgaaga cagaagataa aagcgaaaac
    28981 aggtatgatg tggttgtaac ggtgacgggt taaagcagac cgtgttccgc aaaggagaaa
    29041 acctggctgc caccgactat cagatggtcc ggcacccgga tatccaccag gcccagtgcc
    29101 tgtaccagac gttccgtgat aaggcggtct gccttgctgg gtgtgacttc accggacggg
    29161 tgattgtgcg ccagtaccac ggcagcggca ttgtggtaca gggcgcgttt aatcacttcc
    29221 cggggatgga cttccgtgcg gttgatggtg ccggtgaaga gggtttcacc ggcaatcagc
    29281 ttattctggt tgttcagata cagcacccgg aattcttcac gctccagtcc cgccatgttc
    29341 agaatcagcc attcccgtgc cgcacgggtg gaggtgaagg ccacaccggg ttcatgaaga
    29401 tggcggtcca gggttttcag ggcccgctga atgaggctgc gctcgccggg cgtcatctct
    29461 cctggcagaa aggaaagctg ttgcattgtt ccagcccctt tcagtcaatg atgcgcataa
    29521 tggcgttgca ttccggatgc tgcagggcgt agtcccgcag acggtaataa tgaaccgtca
    29581 tggcataaca ctccgtgcga caggcatgat gactgtacgt catcagacag gcagcaatgc
    29641 cggcggcttc cgggctcatt tcagcacggt taccgttcat ggcattgaac agtacccatg
    29701 tctcgtcatc atcgtcatcc ggttcgggtg ccataaatgc cccgccgttg ttcagggtgt
    29761 acagattcca gataccaccg cagtagtctt cgcaaagatg gtccatccag ccgaagacac
    29821 ggggttccag ggtcacccac tgtggaatga ggccaaaatg ctgcggccag aagctgatgc
    29881 gctgttcatg aggaacgaga gtggcaacca gttgaggctg gtcattctct gaagctgtga
    29941 ctacggaagc agaaagtgta caggtgttct gagaaacagt tttcatgagt gaaatcctta
    30001 taaaacaaat aaatgcaggg ttttcgggga gcagggggta ggggagcctg aatcacgtct
    30061 tcagggatgt gctgcatcag cgcatgttct ccagcagcgt ttctgtcatt acccacaatg
    30121 cgcgattgag cttaatgtcc gtgtcgatgc tgtgaatggc acgggtgtgg atacgttttc
    30181 ctttagcgct gcgaccggaa atcccgcctt tcagcatatt ctcctggatg gtctgatatg
    30241 cgctccacag gtccttaccg taatcctccc ggcgtcgcgg cgtcagaatg tcggcggtgg
    30301 tgacgggctg atgttcgtca ccataacggt aagtcagtgc cgcctgtgcc agcgcctggc
    30361 gtgccggtgg cggcaggacc agcgactgca tggcgtcacg cttttcttca atccggtcaa
    30421 aaacccccac cacctcgtaa gccccttcga taactttctc cactacattt ccccggtgtg
    30481 gaacacgcac ttcccccaga gactgaccac agacgcatcc gttctggcag acgaacctga
    30541 agtaacccgg cagcatctgg tagctggagg taccgtcatg ggagttcagc aggatgattt
    30601 cagggacatg ttgtccgttt atctccccgt cacggcgcag acgcagcatg tgtttggtgt
    30661 atccccggcg gcccgggtcg cgcacacggg tctggcaggc gaagaatggc tgaaaacctt
    30721 cccgctgcag gctttccagc actgtgatgg tggggatcca ggtatagttc tcgcttctgg
    30781 atgtgtgctt atcttccccg aatacgctgg ggacgacctg catcagttct tcacgagtta
    30841 agggacgatc acggcgtatc tgatttacat gaccaaaacg actggctaat tgcatacttt
    30901 gctccttatg aatgagttag cggtataaat gaaaaggccg cgtctcccgg agaagacgcg
    30961 gcctgatgga tgaaatgaag acgtttattg cctgagaatt ccctcactgg cgagatacga
    31021 atttagctgt tttccggcat caggttcaaa attccagcac cgccagcgta gctgcccttc
    31081 ggcattgcgg ataaccagcc ggaaatgcgt tccctggtca tcctcaataa gcacattgcg
    31141 gtaagctgtt gtgacggcaa ccgcctgttc gcgtgagaaa gggccttcgg gcaataaaac
    31201 agggtggcag tccgacatac cggctatttt ccgataaatt caacgctggc gccggtggca
    31261 ttaccgtcag caccaaccgt gacggtcact ttgtcgtacg cacggtcttc aggattgaat
    31321 acttcaaact caaccttatt gccttttact tcgtaagagc caaaatcagc ggtttctgtt
    31381 gtttcctcgt catattcccg agcgtatgcc tgccgagcag cctgcaggac ggaatcttta
    31441 ttaatagtgg tcgtctgttt cggggaaaaa ccggcaagtg atgtgacaaa tttcggacag
    31501 accttcagca tatcaccgcg ggtgaattcc attcgtacac caaatgcctg taaaacggac
    31561 acatcacgct ctgctatgga gtgaacggct tttttgacca tttccggcgc ctgtccgatt
    31621 tccacctcaa cagccctgaa tcccgtctgc tgattacaca tttgtgatgt ggggatcagg
    31681 ttaccgttaa tactgatacg gctgctcagg agcgtctgta caccattcgg gcatccctgc
    31741 atcaggccgg taataccaat cgtgccattt ttcagcatta ccagcgcctg accatcttcc
    31801 gtcatggtct gacaccagcc gtttttgaca gcccagttgc cggcggaggc ggagaatgat
    31861 gccacagtga acagcagggg aaaaagagct ctggtattca tgactgacct ctgaaatgaa
    31921 cgtacggtgt tattgataat gatggaaggt atgcagtgac tgttcaggga atgacgtagc
    31981 cgtatattta gttttctgtt caacatattg tctccatacc tgttgggacg gctttggcag
    32041 gccggcgatc acaggttaaa ggtgttgata agtttatgga tgaaagagaa atgtggcagg
    32101 gcggagataa aacgctcttc agcgaccaga ctgtggatgt ggcctctccg ttcaaaccgt
    32161 tctgatgtca ggctgactga tttgatatct gccagaggaa agacctgcag ttttcccttc
    32221 tgtaacgcga tcagatacca gctaccgctg taataaatga gccggcaggg ctccagtgac
    32281 ggataccatc gctcagccat cagagagatg tggatacact gtcttattgc caggatgagt
    32341 tgcgagaaac actcggggaa cgttgcagtg atatccggcg ccggtagcca gataaggcag
    32401 ggagagggct cctggttgtc ggtaagacag gttatcagtc gtccgttccg gagcggaagt
    32461 atccgtgcga tcccggtatt ctgtataaaa gaaagcattt cagggatcgc acccgggctg
    32521 ctctgtcgtc tgaggctgta cctgccattt ctgtactcta aatccaggtg aaccagacgc
    32581 tgatgaaaat cgcgctgtaa agtacgttct gtaacgccaa attcatctga cagtgttttt
    32641 agtgacagag attctccggt catcagtcgg ctgataatga gtgataagcg aaccgccagg
    32701 cgatcatgcc gggaacgatt atcagacata gatgtgcact ccgtaagaaa acatctttta
    32761 tttcatgatg ttattatgca tcatgatgtt gaaaggatgt gtcgcatccg gataatcagg
    32821 aataaaatgc agcagaaaaa atattctttt aattatctga tttttaagca tgatttcata
    32881 aaaagaagaa tgctcgttaa tccagcgaca catcctgtcg gcgtgagtgt gtcagaacag
    32941 acgctcccac agtgtgatca gcaggtggat aatgcggtcc cgggttttgc gcagaagctg
    33001 taacatccag gccggaaaac tgaaacgggc aacggcgtcg tcaaaacttt tcccgatggt
    33061 ttcaccaaaa gtctgttgtg cgtgtttccg ggcctgttca gagcggtttt ctccccggat
    33121 atgtgaatac accgcgctgg ttgcctgtgg tggcagtgcg tggatcatca gcgacacaaa
    33181 cgccggaagg ttccagccga caggtgcagc aacggagagt actggaaatg atgaagggaa
    33241 cagggttgcc acccgggctg tcaccgtcgc cagtgagagc gcctgctgac gggacgggca
    33301 tttttctgtg gcattccatt cttcagcagg aaacacgcga tcggcatggc tgagaacaaa
    33361 cagaaaacgc gagggatctg cgccttcatt cagtaaaaac tgatgcatgg taatgtcggc
    33421 ggcatacgcg cgttcatctg cccgcaggat ccagataatc agatccagtt ccggtagcag
    33481 ctgacgataa agtgtccggt attcctgatc atgctgcggt gtttcaccaa tgccgggcag
    33541 atcgaccagc gtcattctgc gttcaccgag ttgcagggta agacgatgag cctggcgggt
    33601 gcagccgttc aggggatgcg tggcgcagat acgggactga aaaatggcat tacacaggct
    33661 gcttttgccg gtgccggatt tacccataat gccaatcacg ggctcgtaat gaataacact
    33721 gtggagatgt tcaaggattt tctggcgaat ttcttcagga agaaacgtca gatatgattt
    33781 cacttgtgcg gagagggaag aatggctgaa tgacataccg gtatcctgtt gatttcactg
    33841 aaccggatca cactaatccg gtttatcatt caggtaatat gggcgtgaaa aaatctggag
    33901 tgacagagtg cgggaggtga gatgacggca caacaaagtg atgcgctgcg tgagattgct
    33961 aataaggcca gagttaccac cattcttcag tgtaaggcct ggaaagatac ccagcgaata
    34021 ctgaagcgca gcggactcgt gtgcagagaa cggtcagagc cgtttgatcc ggagaaacat
    34081 tttgactgtt atacggtgcg ttatctgtac ctgcttaaca taatggcact tgagctaaag
    34141 tcagatacac ggattaaggt tgaggtgggg caatggtatc gcatgacggg taaacgttta
    34201 tcacttaatg tcccgccttt tatgctgatc ccgcgtaata tccggagaaa agtggacgga
    34261 ttcagacaat ccagacaatc agaagatgaa gcaaccaaaa atccgcctca accgttcacg
    34321 ggcagtttgt ataaggtttt gagcagggac agcgattcgg ctgagctgga cgcctggttt
    34381 gctgaaccgc cgctgacacg gcaggaagtg tgggagggga ggcgtgttac agattttgat
    34441 ccatgggcgc tgtcttcgtt tatttgccgt tcagaatccc cgacgtttga acttttttac
    34501 caggaatata agcgactggg attaaaatct ctttttgtat caggggtaat gtttgaacag
    34561 tttttaacag ggctaagttt cagaaaatat ggggactggg ttgaatctca gttactggaa
    34621 tcactgggaa atgtgatgtt tttcatgctg ctttatgaca tggaaaatct ggataagttt
    34681 ataaaagaac tgatggacat taatgttcaa agtgaagatt cgaaggaaaa agggaaatca
    34741 cgaaaagaga gaatgctgga gtatatcaac tcatatatcc gcaatgttta tggaagattc
    34801 ctttgtacgt caaaagaaag gtacgaacag cataaacgca aaaacagcag caaaaagaaa
    34861 aacggttctg gtggaactca ttaacgggaa taatcccctg ctgttttctg cataaacaga
    34921 cgatcagctg ataagatgag tttatccagc atttcagcct gcgtcagatt attctttttt
    34981 gccattttcc ggagtgcatt cctggcttcc ggagaaatca gggcacttat ctgtacccgg
    35041 gaatcggttt tctcataccg gaacattttc tggtatgagc acttcatgcg cctgagaaaa
    35101 tcattctgct gataaactaa ttcttttaac ttttgaggtt cagtaccgac agaatagatt
    35161 ttcatgaaga cacggcgcac ataagctatt atgaacagac gtgactcccc tgaagcttta
    35221 aattctgaaa tatcaggaca gtgacgttct tcgtcagtgc aatgtttttg cagatacgcc
    35281 catgcccatt tgatatgtat ccgccgggaa ccaagccatg tcatggggtt cactttttca
    35341 gcatatgacc ataaaaaatg aacgacatac aagaagtcag atacattttc tgtcccggtt
    35401 agccttataa gctcctgtag ccattttctc gtcacacgaa tccttgcctg atgattagct
    35461 ggagctgctt ctgtggcaaa cagtctggca tacagacagg aagttccgta atctgcatat
    35521 tgctcacctg agagtcggac aatactgtca gggacatgat atataaacat ccatacccag
    35581 aatgcggcta cagggtcttt ttgcacccac tccggaacaa cgtctctgcc tgcattatca
    35641 gcattaacag aaggtgattt atcgttatgt cggtaatatt catattccat ccgactaaaa
    35701 acatgatcag attcatctgg tgaatgcaca gacatgaact ccctgggcat aaatgcacaa
    35761 ccaactctat agaactcttt aacccccctc ttatcacctc tgtcagtaaa tttcgcctga
    35821 atcccctttt atccatacat cagaaaaaat gcaataacgt tcatggactc ttttctctga
    35881 tttacata
//
LOCUS       DAJOWB010000036        35499 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJOWB010000036 DAJOWB010000000
VERSION     DAJOWB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.036"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(148..1260)
                     /locus_tag="OHO85_001968"
     CDS             complement(148..1260)
                     /locus_tag="OHO85_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS421 family transposase"
                     /protein_id="HCQ2671344.1"
                     /translation="MNYSHDNWSAILAHIGKPEELDTSARNAGALTRRREIRDAATLL
                     RLGLAYGPGGMSLREVTAWAQLHDVATLSDVALLKRLRNAADWFGILAAQTLAVRAAV
                     TGCTSGKRLRLVDGTAISAPGGGSAEWRLHMGYDPHTCQFTDFELTDSRDAERLDRFA
                     QTADEIRIADRGFGSRPECIRSLAFGEADYIVRVHWRGLRWLTAEGMRFDMMGFLRGL
                     DCGKNGETTVMIGNSGNKKAGAPFPARLIAVSLPPEKALISKTRLLSENRRKGRVVQA
                     ETLEAAGHVLLLTSLPEDEYSAEQVADCYRLRWQIELAFKRLKSLLHLDALRAKEPEL
                     AKAWIFANLLAAFLIDDIIQPSLDFPPRSAGSEKKN"
     gene            complement(1407..2537)
                     /gene="dnaJ"
                     /locus_tag="OHO85_001969"
     CDS             complement(1407..2537)
                     /gene="dnaJ"
                     /locus_tag="OHO85_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCQ2671345.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(2626..4542)
                     /gene="dnaK"
                     /locus_tag="OHO85_001970"
     CDS             complement(2626..4542)
                     /gene="dnaK"
                     /locus_tag="OHO85_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCQ2671346.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQARNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLIEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4919..5323
                     /locus_tag="OHO85_001971"
     CDS             4919..5323
                     /locus_tag="OHO85_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCQ2671347.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            5349..6062
                     /gene="msyB"
                     /locus_tag="OHO85_001972"
     CDS             5349..6062
                     /gene="msyB"
                     /locus_tag="OHO85_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCQ2671348.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            6211..6777
                     /gene="satP"
                     /locus_tag="OHO85_001973"
     CDS             6211..6777
                     /gene="satP"
                     /locus_tag="OHO85_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCQ2671349.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6812..7399)
                     /gene="mog"
                     /locus_tag="OHO85_001974"
     CDS             complement(6812..7399)
                     /gene="mog"
                     /locus_tag="OHO85_001974"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCQ2671350.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(7514..8467)
                     /gene="tal"
                     /locus_tag="OHO85_001975"
     CDS             complement(7514..8467)
                     /gene="tal"
                     /locus_tag="OHO85_001975"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCQ2671351.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            8746..10176
                     /locus_tag="OHO85_001976"
     CDS             8746..10176
                     /locus_tag="OHO85_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HCQ2671352.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            10246..11022
                     /gene="yaaA"
                     /locus_tag="OHO85_001977"
     CDS             10246..11022
                     /gene="yaaA"
                     /locus_tag="OHO85_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCQ2671353.1"
                     /translation="MLILISPAKTLDYQSPLTTMRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(11175..11471)
                     /locus_tag="OHO85_001978"
     CDS             complement(11175..11471)
                     /locus_tag="OHO85_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCQ2671354.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(11685..12971)
                     /gene="thrC"
                     /locus_tag="OHO85_001979"
     CDS             complement(11685..12971)
                     /gene="thrC"
                     /locus_tag="OHO85_001979"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCQ2671355.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12972..13904)
                     /gene="thrB"
                     /locus_tag="OHO85_001980"
     CDS             complement(12972..13904)
                     /gene="thrB"
                     /locus_tag="OHO85_001980"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCQ2671356.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13906..16368)
                     /gene="thrA"
                     /locus_tag="OHO85_001981"
     CDS             complement(13906..16368)
                     /gene="thrA"
                     /locus_tag="OHO85_001981"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCQ2671357.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLTAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(16394..16513)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(16449..16514)
                     /gene="thrL"
                     /locus_tag="OHO85_001982"
     CDS             complement(16449..16514)
                     /gene="thrL"
                     /locus_tag="OHO85_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCQ2671358.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(16728..17414)
                     /locus_tag="OHO85_001983"
     CDS             complement(16728..17414)
                     /locus_tag="OHO85_001983"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCQ2671359.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERAMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(17814..17954)
                     /locus_tag="OHO85_001984"
     CDS             complement(17814..17954)
                     /locus_tag="OHO85_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2671360.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            18050..18766
                     /gene="arcA"
                     /locus_tag="OHO85_001985"
     CDS             18050..18766
                     /gene="arcA"
                     /locus_tag="OHO85_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCQ2671361.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(18825..20177)
                     /gene="creD"
                     /locus_tag="OHO85_001986"
     CDS             complement(18825..20177)
                     /gene="creD"
                     /locus_tag="OHO85_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCQ2671362.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(20235..21665)
                     /gene="creC"
                     /locus_tag="OHO85_001987"
     CDS             complement(20235..21665)
                     /gene="creC"
                     /locus_tag="OHO85_001987"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCQ2671363.1"
                     /translation="MRIGMRLLLGYFLLVAVAVAAWFVLAIFVKEVKPGVRRATEGTL
                     IDTATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDS
                     QGKVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGS
                     RLIGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRY
                     ADSVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAI
                     RGAAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAA
                     LFRRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRIT
                     LSAEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARL
                     FNGEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(21665..22354)
                     /gene="creB"
                     /locus_tag="OHO85_001988"
     CDS             complement(21665..22354)
                     /gene="creB"
                     /locus_tag="OHO85_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCQ2671364.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(22367..22840)
                     /gene="creA"
                     /locus_tag="OHO85_001989"
     CDS             complement(22367..22840)
                     /gene="creA"
                     /locus_tag="OHO85_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCQ2671365.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            23051..23920
                     /gene="robA"
                     /locus_tag="OHO85_001990"
     CDS             23051..23920
                     /gene="robA"
                     /locus_tag="OHO85_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCQ2671366.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23917..24564)
                     /gene="gpmB"
                     /locus_tag="OHO85_001991"
     CDS             complement(23917..24564)
                     /gene="gpmB"
                     /locus_tag="OHO85_001991"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCQ2671367.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            24616..25128
                     /gene="yjjX"
                     /locus_tag="OHO85_001992"
     CDS             24616..25128
                     /gene="yjjX"
                     /locus_tag="OHO85_001992"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCQ2671368.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            complement(25171..25497)
                     /gene="trpR"
                     /locus_tag="OHO85_001993"
     CDS             complement(25171..25497)
                     /gene="trpR"
                     /locus_tag="OHO85_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCQ2671369.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(25587..27524)
                     /gene="sltY"
                     /locus_tag="OHO85_001994"
     CDS             complement(25587..27524)
                     /gene="sltY"
                     /locus_tag="OHO85_001994"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCQ2671370.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            27735..29402
                     /gene="ettA"
                     /locus_tag="OHO85_001995"
     CDS             27735..29402
                     /gene="ettA"
                     /locus_tag="OHO85_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCQ2671371.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(29709..30941)
                     /gene="nadR"
                     /locus_tag="OHO85_001996"
     CDS             complement(29709..30941)
                     /gene="nadR"
                     /locus_tag="OHO85_001996"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCQ2671372.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30962..32344)
                     /gene="radA"
                     /locus_tag="OHO85_001997"
     CDS             complement(30962..32344)
                     /gene="radA"
                     /locus_tag="OHO85_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCQ2671373.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(32393..33361)
                     /gene="serB"
                     /locus_tag="OHO85_001998"
     CDS             complement(32393..33361)
                     /gene="serB"
                     /locus_tag="OHO85_001998"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCQ2671374.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            33467..34111
                     /locus_tag="OHO85_001999"
     CDS             33467..34111
                     /locus_tag="OHO85_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCQ2671375.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            34139..35155
                     /gene="lplA"
                     /locus_tag="OHO85_002000"
     CDS             34139..35155
                     /gene="lplA"
                     /locus_tag="OHO85_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCQ2671376.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            35187..35336
                     /locus_tag="OHO85_002001"
     CDS             35187..35336
                     /locus_tag="OHO85_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2671377.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
BASE COUNT         8353 a         9666 c         8830 g         8650 t
ORIGIN      
        1 accatctgaa gaatgcgacg cctcggtgcc tcgttaagac gatgcctcgc gttcttcaat
       61 tgcgttttgt aggctgtcag ggatactgtc ccacgaatgg ccacctgtaa gctccagatg
      121 accatttttg ttattctcca caacgagtta gttcttcttt tcggatccgg cacttctggg
      181 ggggaaatcc agcgatggct ggattatgtc gtcaattaaa aatgcggcga gtagattagc
      241 aaatatccac gctttcgcga gttcaggttc ctttgcacgc aaagcatcca ggtgcagcaa
      301 acttttgagc cgcttaaaag ccagttcaat ttgccatcgc agacggtaac aatcagccac
      361 ttgctctgct gaatattcat cttccggtaa tgatgttagc aatagcacat ggcccgctgc
      421 ttccagcgtt tccgcctgaa ctactcgtcc ttttcgacga ttctcgctga gcagccgggt
      481 tttactgatt aatgcttttt cgggaggaag tgatacggca atgagacgtg ccggaaaggg
      541 agctccggct tttttattac ctgaattgcc tatcattaca gtggtttcac cgttcttacc
      601 gcaatccagc ccgcgcagaa aacccatcat gtcaaagcgc attccttctg cagttaacca
      661 gcgcaatcct cgccagtgaa cccggacgat ataatcagct tctccaaaag caagtgagcg
      721 gatacattcg ggacgcgaac cgaatccccg gtcagcaatg cgtatctcgt ctgccgtttg
      781 cgcaaatcgg tccagccgtt cagcgtctct gctgtcggtt agctcaaaat cagtgaactg
      841 acaggtatga ggatcatatc ccatatgtag tcgccattca gcgctgccgc ccccgggcgc
      901 actgattgct gttccatcga caagacgcaa tctctttccg cttgtacaac ccgtaactgc
      961 ggcgcgtaca gcaagtgttt gtgcggcaag tatgccaaac cagtcggcgg cattccgcag
     1021 ccgcttcagg agagccacgt cagataatgt tgcaacgtca tggagctgag cccatgcagt
     1081 gacttcacgt aatgacatcc ccccggggcc gtaagccagc cccagacgta gcagagttgc
     1141 agcatcacga atttcgcggc ggcgggttag agccccggca ttacgtgccg aagtatccag
     1201 ttcttcgggc ttaccaatat gggccagaat tgctgaccag ttatcgtgag agtaattcat
     1261 cggcacgtta aatcatatca ggcgtaatac cacaaccctt aagttagcgc ttatgggagt
     1321 gatccccgcc actttacagg tgctcgcata tcttcaacgc accctatttt tacccaggcc
     1381 tgcccacggg caggcttttg gggaggttag cgggtcaggt cgtcaaaaaa cttcttcaca
     1441 ccatcaaaga agctctttga gcgcgggctg ttgtgctcgc cggttgggcc accgaagctt
     1501 tcttgcagct cttgcagcag ctgtttctgc ttctcgttca ggcctaccgg tgtttcgacg
     1561 acaacgcggc acagtaaatc accctgtgcg ccaccgcgga cagacttgac gcctttaccg
     1621 cgcatacgga acagcttacc agtctgggtt tcgccaggca ctttcagttt gacgcgacca
     1681 tcaagggtcg gtacttcgat ttcgccaccc agcgccgcca tagcgaagtt gatcgggact
     1741 tcgcaataca ggttgttgcc ttcacgctcg aaaatcgggt gctgtttaac ctgaacctga
     1801 acgtacagat cgcctgccgg tgcgccatgc tcgcccgctt caccttcgcc cgcaagacgg
     1861 atgcggtctc cagtgtccac ccctgccggg attttaacgg acagcgtttt gctgcgctca
     1921 acacgaccat gaccatgaca tttgttgcac ggatctttga tcagcgtacc gcgaccctga
     1981 cagtgtggac aggtctgctg cacggcaaag aaaccctgac gcatctgcac ctgaccagaa
     2041 ccatgacagg tcggacaggt ctgcggctgt gtacctggtt ttgcaccgct accgtggcaa
     2101 acgtcacact cttccagagt cggaatgcgg atctctttgg tcacgccacg tacagcttct
     2161 tcgagggtga gctccatgtt atagcgtaaa tcagcaccgc gcgccgcacg ttgacgacca
     2221 cgtccgccgc caaaaatatc gccgaaaacg tcaccaaaaa tatcgctgaa gtctgcgccg
     2281 ccgccaaaac cgccgccgcc cataccacct tgctcaaacg cagcatgacc atactgatcg
     2341 tatgccgcac gtttttgcga gtcggtcaga acttcataag cttccttgat ctctttaaat
     2401 ttcgcctcgg cctctttgtc accctggtta cggtccgggt ggtatttcat ggccaggcgt
     2461 ttgtaggcct ttttgatttc acgctcttcc gctgttttgg aaacgcctaa aatctcgtaa
     2521 taatcttgct tagccatctt ttttaaattg cccctagatg aatgcacggg cggagaggaa
     2581 attccccttc gcccgtgtca gtataattac ccgtttatag ggcgattatt ttttgtcttt
     2641 gacttcttca aattcagcgt cgacaacatc gtcatctttc gcgttgtttg cagaagcatc
     2701 agcaccggca gtctgctgct gggcatgttg ctgctgggcg atttcaatca gtttctggga
     2761 aacctgtgcc agctcctgca ttttcgcttc gatagcggct ttgtcttcac ctttcagagc
     2821 agtttccagt gcagtcagtg cagactcgat agcagttttg tcgtcagccg gcagtttgtc
     2881 gcctgcttct tcaacctgct tacgggtgct gtgcagcaga tggtcgccct ggttgcgagc
     2941 ctgtaccagc tcttcaaact tacggtcagc ttcggcgtta gcttctgcgt cgcgtaccat
     3001 tttctggatt tcatcttcgt tcagaccaga agaagccttg atggtgatct tctgctcttt
     3061 accgctgttt ttatctttcg cggaaacgtg caggataccg tcagcatcga tatcgaaggt
     3121 aacttcgatc tgcggcatgc cgcgcggtgc cgggttgata ccatccaggt tgaactgacc
     3181 cagagatttg ttatcagccg cacgtttacg ttcaccctgc agcacatgga tggttaccgc
     3241 agactggttg tcttcagcgg tagagaacac ctggctgtgc ttggtcggga tagtggtgtt
     3301 tttcgcgatc agcgtcgtca tcacaccgcc catggtttcg atacccagag acagcggggt
     3361 aacgtccagc agcagtacgt ctttcacgtc accagtcaga acaccaccct gaacagcagc
     3421 accgattgct acagcttcgt ccgggttaac gtctttacgc ggctctttac caaagaactc
     3481 agcaactttc ttctgaacca ttggcatacg agtctgacca ccaacgagga taacgtcgtc
     3541 gatatcagat acggacaggc cagcgtcctg cagtgcaact ttcagcggct caatggaacg
     3601 gtttaccaga tcttcaacca ggctttccag tttcgcacga gtcactttga tgttcatgtg
     3661 tttcggaccg gtcgcatctg cagtgatgta cggcaggtta acgtcggtct gctgagcgga
     3721 agacagttcg attttcgctt tttctgccgc ttctttcagg cgctgcattg ccagcggatc
     3781 gttgcgcagg tcaatgccct gatctttctt gaattcttca accaggtagt tgatcagacg
     3841 gctgtcgaag tcttcaccac ccaggtgggt atcaccgttg gtagccagaa cttcgaaggt
     3901 tttttcgccg tcaacttcgt cgatttcgat aatagaaata tcgaaagtac caccacccaa
     3961 gtcataaacc gcgatagtac ggttgccagt acctttgtcc agaccgtaag ccagcgcagc
     4021 tgcggtcggt tcgttgatga tacgttttac ttccagacca gcgatacggc ctgcgtcttt
     4081 ggttgcctga cgctgagcat cgttaaagta tgccggtacg gtgataacag cttcagttac
     4141 cggttcaccc aagtaatctt cagcggtttt cttcattttt ttcagcactt cagcagaaat
     4201 ctgcggcggt gccattttct ggcctttaac ttcgacccat gcgtcgccgt tatcagcagc
     4261 aataattttg aacggcatga tggaaacatc acgctgtact tcttcgtcct ggaagcggcg
     4321 gccaatcagg cgtttaatcg caaacagggt gttttgcggg ttcgtcactg cctgacgttt
     4381 agccggctga ccaaccagag tttcaccatc ctgggtatag gcaataatag aaggcgtggt
     4441 gcgatcgcct tcggcgttct ccagcacgcg aggagtggtg ccatccataa tcgctacaca
     4501 agagttggta gtacccaggt cgataccaat tattttaccc atctaaacgt ctccactata
     4561 tattcggtca tcatgtggtt gtgagtctgt aataggggcg aaacgtctgg tttcaactgc
     4621 ccaatttcat ttttttgcag actcactcac tgcggttgac tactaaatgg ggtcgtaacc
     4681 cacgtcatca agggggagat gcaaaaattt tttgtgcttt tgcgcagaaa tttcgcgata
     4741 tagcaggaca agacgtgaaa ctgacagcaa ttttattcat ataaagaatg aatcgcagcc
     4801 aagacattca tcaaaaaatt gtaataaaaa gaaaacatta cgcacctgaa tcttctcttt
     4861 atcagcagta aactagtggg tatccatccc cctacctctc cccactaaga gaatccttat
     4921 gaaatccgtt tttacgattt ccgccagcct ggcgattagt ctgatgctgt gctgcacggc
     4981 gcaggcaaac gaccataaaa tcctcggcgt cattgcgatg ccacgtaacg aaaccaacga
     5041 tctggcgctg aaacttcctg tttgccgaat tgttaaacgc atacaactct ctgccgacca
     5101 tggcgattta cagttaagcg gtgcatcggt ttatttcaaa gccgcccgta gcgccagtca
     5161 gagcctgaat attccttcag aaataaaaga agggcaaacc actgactgga tcaacattaa
     5221 cagcgataac gacaataaac gctgcgtctc aaaaatcacc ttttccggtc atacggtgaa
     5281 ctcatcggat atggccacgc tgaaaattat cggcgacgac taacgccaga atatagaagc
     5341 cacaaaaaat gaatgttaat tacctgaatg attcagatct ggattttctc cagcattgca
     5401 gtgaggaaca gttggcaaat ttcgcccgat tgctcaccca taatgaaaaa ggcaaaactc
     5461 gcctctccag cgtactgatg cgcaacgaac tgtttaaatc gatggaaggg catcccgagc
     5521 aacatcgccg caactggcag ctgattgctg gagaattaca gcattttggt ggcgatagta
     5581 tcgccaacaa actgcgcgga cacggtaaat tgtatcgggc cattttgctc gatgtttcaa
     5641 aacgattgaa gctgaaagcc gacaaagaga tgtctacgtt tgagattgag caacagttac
     5701 tggaacaatt tctgcgtaac acctggaaga atatggacga ggaacataaa caggagtttc
     5761 tgcacgcggt cgatgcgagg gtgaatgagc tggaagagct gctgccgctg ctgatgaaag
     5821 acaaattatt ggcaaaaggc gtgtcgcatt tgctttccag ccaactgacc cgcattttac
     5881 gcacccacgc agcaatgagc gtacttgggc atggtttgct gcgcggcgcg gggctgggag
     5941 gccctgtagg tgcggcacta aatggggtta aagcggtcag cggcagcgcc tatcgcgtga
     6001 cgattccagc cgtactgcaa atcgcctgcc tgcgccggat ggttagcgcc actcaggtct
     6061 gataccggaa accggggaaa attttcattt tcccctcaaa agatcgtaga cactgcccca
     6121 ctggctgatt attatgccgc gccctgaaaa cactacagtt attcagggaa attatttcac
     6181 cattcattcg atgatgattt ttgaggaatt atgggcaaca ctaagttggc taatccggca
     6241 ccgctgggcc tgatgggctt cggcatgacc accattctgc ttaacctgca caacgtgggt
     6301 tatttcgctc tggacggtat tattcttgcc atgggcattt tctacggcgg catcgcgcaa
     6361 atttttgctg gtctgctgga gtacaaaaaa ggcaacactt tcggtttaac cgcattcacc
     6421 tcttacggtt ctttctggct gacgctggtt gcgattctgc tgatgccgaa actgggtctg
     6481 accgatgcgc caaatgcaca gttccttggt gtctatctgg gtctgtgggg cgtatttacg
     6541 ctgtttatgt tcttcggcac gctgaaaggc gcacgcgttc tgcaattcgt tttctttagc
     6601 ctgaccgtgc tgtttgccct gctggcgatc ggtaacattg ccggtaacgc cgcaatcatc
     6661 cactttgccg gctggattgg gctgatctgc ggtgccagcg caatctatct ggcgatgggt
     6721 gaagtactga acgagcagtt tggtcgcacc gttctgccga ttggtgaatc ccactaattg
     6781 ttttgagatc cccccgctcg gggggatttt tttattcgct aacgtcgcgt cttgcactct
     6841 tcggtctgaa tgctgcaacc acttccggtg ccgtttcaac gtatggccct tccagcaact
     6901 gaatgcagta cggtacgctg gcaaaaatac cgtgtaccac aacgttaccc gcagcgtcct
     6961 tcacaccttc cagcgtctct ttaatagact tcggctgacc gggtaagtta aggatcagcg
     7021 cctgtttgcg aatcacacca acctgacgcg aaaggatcgc agttggtaca aaatgcaggc
     7081 ttatctggcg catctgttca ccaaagccag gcatctcgcg gtccgctact gccagcgtcg
     7141 catcgggcgt tacgtcacga cgcgccgggc cagttccgcc cgtggtgagc accagatggc
     7201 aactcatttc atccactagc tcacacaacg tttgctcgat gatcgcctgc tcatcgggga
     7261 ttaagcgggt ttccagttca aacggcgtgg ttagcgccga tgtcagccat tcttccagcg
     7321 cagggatgcc tttatcctga taaacgccgc tggatgcgcg atcagagatg gaaactaagc
     7381 caatacgtaa agtattcatc aagatttttc cgtttaaaca gtctcgttaa acagaatgat
     7441 acactgcgaa gggagtgaca gacaggcttc agaagaggta gcgtgaccga cttcccggtc
     7501 acgctaagaa tgattacagc agatcgccga tcattttttc cagtttttcc tggtcaatag
     7561 caaacttacg gataccttcc gccagtttat ctactgccat tggatcctgg ttgtgctgcc
     7621 acaggaactc ggactcagtg atacgcgccg gacgcgcttt cacttcgccg gtgtaagaca
     7681 gtttacgttc gatagcccct tcgctctcag ccagctcttt cagcagtgcc ggtgcgatgg
     7741 tcagacggtc gcagcctgcc agttccagaa tttcgccgat gttacggaag cttgcgccca
     7801 taaccacggt ttcataaccg tgctctttgt agtactggta gatttcagat acagaaacca
     7861 cgcccggatc ttctgccgga gcgtactctt tcttatcggt attcgctttg taccagtcaa
     7921 gaatacggcc aacaaacggc gagatcagga acacgcccgc ttccgcacaa gcacgagcct
     7981 gagcgaagga gaacagcagg gtcaggttac agttgatgcc ttctttttcc agctgttctg
     8041 cagcacggat accctgccag gtagaagcca gtttgatcag aatacgatcg ttgctaatac
     8101 cagcatcgtt gtagagtttg atcaggcgtt ttgctttcgc aattgacgct tcagtgtcat
     8161 aggaaagacg cgcatcaact tcagttgaga tacggcccgg aaccagtttc aggatttcca
     8221 gaccaatatt tactgccagt ttgtcggtcg cgtccacgat ctgctgcgcg cgatcgttgc
     8281 tctgctgttt cgcccaggcg acagcatcat caatcaactt acggtattcc ggaatctgcg
     8341 ctgcgttaag aatgagagaa gggttggttg tggcatcctg cggttgatac agcttcattg
     8401 ccgcgatgtc cccagtgtcg gccactacgg tggtgtactg acgaagggag gtcaatttgt
     8461 ccgtcatgat agtatttctc tttaaacagc ttgttagggg gatgtaaccg gtctgccctg
     8521 atgatatcac gacgcaacgt cagcgcaacc gccgacaaga cggttatcgc caggcgagac
     8581 tgtttcggat ttctgacact gcgttgatat cgcccgccat ttttatacaa aacctcatgt
     8641 atgctacgca gaagttatca agtacctcgt agcgtatata cttcttaaac aattggtaac
     8701 gtttacacag gaaagtcatc gcgaccggca ataagaggga tatgcatgcc agattttttc
     8761 tcctttatta acagcgtcct ttggggatcg gtaatgattt acctgctctt cggcgcaggt
     8821 tgttggttca cttttcgcac cggatttgtg cagtttcgct acatccgcca gtttggcaaa
     8881 agtcttaaaa atagcattca tccacagcca ggcggtttaa cctcatttca gtcattgtgt
     8941 accagtcttg cggcgcgcgt gggtagcggc aacctggccg gcgttgcgct ggcaattacc
     9001 gccggtggac ctggagccgt cttttggatg tgggttgccg cgtttatcgg catggcgacc
     9061 tcgtttgccg aatgttctct ggcacaactt tataaagaac gtgacgtcaa tggacagttt
     9121 cgtggcggac cggcatggta tatggcgcgc gggctgggga tgcgttggat gggcgttctg
     9181 ttcgccgtct ttttgctcat cgcctatggc ataattttca gcggagttca ggcgaacgcc
     9241 gttgcccgcg ccctaagttt ttcttttgat tttcctccgc tggtgacagg cattattctc
     9301 gctgtctttg ctctgctggc aatcactcgc ggtcttcatg gcgtcgcccg gctcatgcag
     9361 ggttttgtcc cgttgatggc gataatctgg gtactgacca gcctggtgat ttgcgtaatg
     9421 aatatcgggc aacttcccca cgtcatttgg tctatttttg agagtgcttt tggctggcag
     9481 gaagcggcag gcggcgcggc gggatatacc ttaagccagg cgattactaa cggttttcag
     9541 cgcagtatgt tttccaatga ggcgggaatg ggttctacac caaacgcagc agcggcagcg
     9601 gcgtcctggc ctccgcatcc ggcagcgcaa gggattgtcc agatgattgg catttttatc
     9661 gacaccctgg tcatctgtac ggcaagcgcc atgctgatat tactggcggg taacggcaca
     9721 acctacatgc cgctggaagg tattcagctt atccagaagg cgatgcgggt gctaatgggt
     9781 tcctggggtg ctgagtttgt taccctcgtg gttattctgt ttgccttcag ctccatcgtt
     9841 gccaactaca tttatgccga aaacaatctc ttctttttac gcctgaacaa ccctaaagcg
     9901 atctggtgtt tgcggatctg caccttcgca acggtcatcg gcggcacctt gctaagtctt
     9961 ccgctgatgt ggcaactggc agatatcata atggcctgca tggctattac caatttgacc
    10021 gccattttac tgctctcgcc tgtggttcat accattgcca gtgattatct acgccagcgt
    10081 aaactcggcg tgcgcccggt gtttgatccg ttgcgttatc cggatatcgg tcgccagctt
    10141 tctccggacg cgtgggatga tgtttcgcag gagtaatcac aactatcgat caactcattc
    10201 tctttttttg ctaaagtcgg cataaatttc ctgcaaggac tggatatgct gattcttatt
    10261 tcacctgcga aaacgcttga ttaccaaagc ccgttgacca ccatgcgcta tacgctgccg
    10321 gagctgttag acaattccca gcagttgatc catgaagcgc ggaaactgac gcctccgcag
    10381 attagcacgc tgatgcgcat cagcgacaaa ctggcgggta tcaacgccgc tcgctttcat
    10441 gactggcagc cagatttcac gccggagaat gcccgccagg cgattctggc gtttaaaggt
    10501 gatgtctaca ccggcttgca ggccgaaacc ttcagcgaag acgatttcga ttttgcccaa
    10561 cagcatttgc gaatgctttc cggcttgtat ggcgtactgc gcccgctcga tttaatgcag
    10621 ccttatcgtc tggaaatggg gatccgtctt gagaatgccc gagggaaaga tctgtatcaa
    10681 ttctggggag atatcatcac caacaagctg aacgaggcgc tcgcagcaca aggcgataat
    10741 gtggtgatta atctggcgtc agatgagtat tttaaatcgg tgaagccgaa gaaattgaat
    10801 gccgagatta tcaagccggt gttcctcgat gagaagaacg gcaaatttaa gataatcagc
    10861 ttctacgcta agaaagcacg cggtctgatg agtcgtttta ttattgaaaa tcggctgacc
    10921 aaaccagagc aactgactgg ttttaatagc gaaggttact tctttgatga agcttcctcc
    10981 agcaatggcg aactggtgtt taaacgctac gagcagcgtt aagtttgtag tcaatgccgg
    11041 atgcggcgtg aacgccttat ccggcctaca aaagcatgca gattcaatat attgcagaat
    11101 tcacgtaggc ctgataagac gcgccagcgt cgcatcaggc gctgaatgcc ggattgttac
    11161 ccggcatttg tcatttagcg gtgatgtttg cctggaccat gaccgccgtg atgatcatga
    11221 ggagctttct tatggtggcg cggcggtggc ggcggtccgt gtgggtgcca gcgattgcct
    11281 cgccattcat aatgttgttt ccaccagccg tggtcgcgcc agtgacctcc atcccagtaa
    11341 tagccacgat tatcacgatc gcctatctgt aattttactg acgggactaa cgtaatttcc
    11401 gcagcctgtg ctgccatggg agcgaccaga accagggaaa gtgcgagtac gatagattgc
    11461 atctttttca caggttactc cttttatcat tgcccgttat gggtcgatat ccggagatta
    11521 cgcgatgaga acgacggttg ttattctccg caatcctaat ctctaagtta ccgcatttat
    11581 tgagaatttc tcctttttcc ctgtcgtttt tctccataat ttcttcataa aaaagccggg
    11641 ctgcataaaa gcaaacccgg cctgattgag ataatgaata gattttactg atgattcatc
    11701 atcaatttac gcaacgcagc aaaatcggcg ggcagattat gtgaaagcaa gggtaaatca
    11761 gcacgttctg ccagctcttt tggcagatcc aacgtttcac cgagaatcgc ttccacgctc
    11821 tctttaaatt tcgccggatg cgcggtgccg aggaacaagc catattcgcc tggattcaac
    11881 tggtcacgca gcgcacgata cgctacggca gcgtgcggct ccgaggtgta gcccagttct
    11941 tttaactcac gcattgtctg ttgcgtggtt tcatcatcca cggctgcata acccagctct
    12001 ttcagttgcc agattttgcg gcggaacaac tcttccacac gcggccagtt gttcggctga
    12061 ctcacgtcca tcgcgttgga taacgtcgcc tgagtcgctt tgggtgacca ctgaccgtcg
    12121 tgcaggaaac gtggcacggt atcgttcacg ttggtcgcag caataaaacg tttcaccggc
    12181 agaccgagtg acttcgccag cagacccgcc gtcaaatcgc cgaagtttcc gcttggcacc
    12241 gagacaacca gctggttgcg cgcttcctgc ggcagctgcg caacagcttc aaagtagtag
    12301 caaatctgcg ccagcaaacg gctgatgtta atcgagttag ctgagtttaa ccccagcgcc
    12361 actttcagtt cttcatcatc aaacgcctgc ttcaccagcg cctgacaggc atcgaaatcg
    12421 ccgtcgatgg caacagtttc gatattgccg cccaacgtac agaacagttt ttcttgcagt
    12481 ggactgattt tgcctcgtgg atagaggata accactttca cattcggtaa accgtagaaa
    12541 gcatgagcca ctgccgctcc ggtatcaccg gaggtcgcgg tcagaatggt cactggctta
    12601 tcgcccgcaa tatgggtcag catttgtgcc ataaagcgac cgccgaaatc tttaaatgcc
    12661 agcgttggcc cgtggaacaa ttccagacaa ccgacatcgc tttcaacatt ggcgaccgga
    12721 gccgggaagg caaacgccgc gcgcacgcgc tcttccagga tttcctgcgg gatttcatca
    12781 ccaataaacg ccgagaggat cttcgcactg cgggtgacaa aatccagctt cagcatctca
    12841 tcaatttcag tcaggctgaa ttccggcagg tcatgcggaa aaaacagccc ctgatttttg
    12901 cccaacccct gggttacggc ttgcgcaaag ctgacctgct cgttgtgatc tttcagattg
    12961 tagagtttca tttagttttc cagtactcgt gcgcccgccg tatccagccg gcaaatatga
    13021 acaaaacctt cctgattttg caggtagttc ttacccaacc agtcggcaac gcgctgggcg
    13081 gtatccggct tgtcacatag agcgaacaag gtcgggccgg agccggagat accgctcgct
    13141 accgcgccga tttccgcgac cgcctgccgc gcctgccgga agccaggcag taaccgttca
    13201 cggtagggtt cagcgataac atctttcatc agcttcgcgg caagctcagg ctgacgggaa
    13261 tagcaggcgt gaatgaagcc agccagatgt cgcccgtgcg caatgcaatc ctggcggcga
    13321 tactgcgccg gtaaaatagc cctggcttct gccgtcgaga ctttaatccc cggatacgcc
    13381 agcacccaca gccactcatc aaaccctggc acttgctggc tgatgatgtc gttttcttcg
    13441 atcatcaact gcataccacc aagaaaacac ggtgccacgt tgtcgtaatg aatgctgccg
    13501 gagattcgtc cttccagctc gcccatcaaa gccagcaaac gagtgtcatt aagcggcttg
    13561 ccacagtgtt cattcatcgc catcagcgcc gcgaccaccg aacaggcgct ggagcctaag
    13621 cccgaaccga ttggcatatt cttttccaga gtcatcgcca ctggaatttg cttgccaagc
    13681 tcctggcaaa aacgctccca gcactgataa acgatatttt cccgtggttc tgacggcagc
    13741 ttatcggcaa agcgtccgag gttgttgaga ctgaatgtct ctgccgcctc aaccgtgact
    13801 acatctccga gcaatgcacc atcaacaggt gtcaccgccg ccccgagcac atcaaacccg
    13861 acgctcatat tggcactgga agccggggca taaactttaa ccatgtcaga ctcctaactt
    13921 ccatgagagg gtacgtagca gatcggcaaa gacaccggca gctgtaacgt cattgcccgc
    13981 accatatccg cgcagcacca acggcagcgg ctgataatag tggctataaa aggccagggc
    14041 gttttcgcca tttttcactt tgaacagcgg atcattacca tccacttcgg caatcttcac
    14101 gcggcaggca ccatcttcat caatattgcc aacatagcgc aaaacttttc cttcatcacg
    14161 ggccttcgcc acgcgcgcgg caaagagatc gtcgagctgt gacagattcg ccataaaagc
    14221 ggcaacatca ccctcagcgt taaactctgc gggcagcaca ggttcaattt caatatccgc
    14281 cagctccagt tcacgtcccg tttcacgagc gagaatcaat agcttacgcg ctacatccat
    14341 accagaaaga tcatctcgcg gatccggttc ggtataaccc atttcccgcg ccagagtagt
    14401 cgcctcggag aaactcatgc cttcgtctaa cttgccgaag atataagaaa gcgaacctga
    14461 aagaatgccg gagaacttca tcaattcatc accagcattg agcagatttt gcaggttctc
    14521 aataaccggt aatccagccc caacgttggt gtcatagagg aatttacgcc gcgatttttc
    14581 cgccgcatga cgcaacagat ggtagtaatc catcgacgag gtgttggcct ttttgttcgg
    14641 cgtgacaacg tggaaacctt cgcgcaagaa gtcggcatat tgatccgcca ctgcctggct
    14701 ggaggtgcag tcaacaatga ccgggttcag cagatgatat tctttcacga ggcgaattaa
    14761 gcgcccgaga ttaaacggct ctttggcttg cgccagttct tcctgccagt tttccagatt
    14821 taggccatgc acattggtga gcagtgcctt cgagttggca acaccgcaga cacgtaagtc
    14881 gatatgttta ttcttcagcc agctttgttg acgcttcagt tgctccagca gcgcaccgcc
    14941 aacgccaccg acgccaatca caaacacttc gataacctga tcggtattga acagcatctg
    15001 atgagtaacg cgcacgccag tggtcgcatc atcgttattt accacgacag agattgagcg
    15061 ttcagaagat ccctgagcaa tggcgacaat gttgatattg gcgcgggcca gcgcggcaaa
    15121 gaatttcgcc gagatcccac gcaaggtgcg cataccatca cctaccaccg agataatggc
    15181 cagccgttcc gtcaccgcca gcggctccag taagccttct ttcagttcca ggtagaactc
    15241 ttcctgcatt gcccgttcag cccgcacaca gtcgctttgc ggaacgcaga aactgatact
    15301 gtattcggaa gatgattgcg taatcagcac cacggaaata cgggcgcgtg acatcgcggc
    15361 aaagacgcgc gccgccatgc cgaccatccc tttcattccc ggaccagaaa cgctgaacat
    15421 tgccatgtta ttcagattgg aaatgccctt gaccggtaat tcgtcttcat cacggctggc
    15481 accaatgagc gtacctggtg cttgaggatt tccggtattt ttaatcaggc aagggatctg
    15541 gaactgggcg atgggggtaa tggtgcgggg gtgaagaact ttagcgccga agtaggaaag
    15601 ctccatcgct tcctggtagg acatcgactt caacaacctc gcatcgggca cctgacgcgg
    15661 gtcgcaggta tagaccccgt caacgtccgt ccaaatctcg caacaatcgg cgcgtaaaca
    15721 ggcagccagc accgccgcgg agtagtcgga accgttgcgt ccaagtacca ccagttcgcc
    15781 tttttcatta ccggcggtga agcctgccat cagcaccatg tgatcagccg gaatacgact
    15841 tgccgcaata cggcgggtgg actcagcaat atcgacggta gattcgaggt aatgccccac
    15901 tgccagtagt ttttcgaccg gatcgataac ggtaacgttg tgaccacgcg cttctaacac
    15961 gccggccata atggcgatcg acattttctc gccacggcaa atcagcgcag cgttgatgct
    16021 atccgggcac tgccccaaca aactaatgcc atgcaggaca tgttttattt gggcaaattc
    16081 ctggtcgacg aaagttttca gttgcgccag cgggaatccc ggctgggcgg cggtgagtcc
    16141 cgtcagaagt tcggcaaaaa tacgttcggc atcgctgata ttgggtaaag catcctggcc
    16201 gctaatggtt ttttcaatca tcgccaccag gtggttggtg attttggcgg gggcagagag
    16261 gacggtggcc acctgcccct gcctggcatt gctttccaga atatcggcaa cccgcagaaa
    16321 acgttctgca tttgccactg atgtaccgcc gaacttcaac actcgcatgg ttgttacctc
    16381 gttacctttg gtcgaaaaaa aagcccgcac tgtcaggtgc gggctttttt ctgtgtttcc
    16441 tgtacgcgtc agcccgcacc gttacctgtg gtaatggtga tggtggtggt aatggtggtg
    16501 ctaatgcgtt tcatggatgt tgtgtactct gtaattttta tctgtctgtg cgctatgcct
    16561 atattggtta aagtatttag tgacctaagt caataaaatt ttaatttact cacggcaggt
    16621 aaccagttca gaagctgcta tcagacactc tttttttaat ccacacagag acatattgcc
    16681 cgttgcagtc agaatgaaaa gctgaaaaat acttactaag gcgtttttta tttggtgata
    16741 tttttttcaa tatcatgcag caaacggtgc aacattgccg tgtctcgttg ctctaaaagc
    16801 cccaggcgtt gttgtaacca gtcgaccagt tttatgtcat ctgccactgc cagagtcgtc
    16861 agcaatgcca tggctcgttc gcgtaaagct tgcagttgat gttggtctgc cgttgtgtca
    16921 cttttcgccg gttgttgtat taatgttgct aattgatagc aatagaccat caccgcctgc
    16981 cccagattga gcgaaggata atctgccacc atcggcacac cagtaagaac gtcagccaac
    17041 gctaactctt cgttagtcag cccggaatct tcgcgaccaa acaccagcgc ggcatggctc
    17101 atccatgaag atttttcctc taacagcggt accagttcta ctggcgtagc gtagtaatga
    17161 tatttcgccc gactgcgcgc agtggtggcg acagtgaaat cgacatcgtg taacgattca
    17221 gccagtgtcg ggaaaacttt aatattatca ataatatcac cagatccatg tgcgacccag
    17281 cgggtggctg gctccaggtg tgcctgacta tcgacaatcc gcagttcgct aaaacccatc
    17341 gttttcattg cccgcgccgc tgccccaata ttttctgctc tggcgggtgc gaccagaata
    17401 atcgttatac gcatattgcc actcttcttg accaaataac cgcgaacagg gtgatcactg
    17461 tcaacttatt acgcggtgcg aatttacaaa tccttaacgt aagtcgcaga aaaagccctt
    17521 tacttagctt aaaaaaggct aaactatttc ctgactgtac taacggttga attgttaaaa
    17581 aatgctacat atccttctgt ttacttagga taattttata aaaaataaat ctcgacaatt
    17641 ggattcacca cgtttattag ttgtatgatg caactagttg gattattaaa ataatgtgac
    17701 gaaagctagc atttagatac gatgatttca tcaaactgtt aacgtgctac aattgaactt
    17761 gatatatgtc aacgaagcgt agttttattg ggtgtccggc ccctcttagc ctgttatgtt
    17821 gctgttaaaa tggttaggat gacagccgtt tttgacactg tcgggtcctg agggaaagta
    17881 cccacgacca agctaatgat gttgttgacg ttgatggaaa gtgcatcaag aacgcaatta
    17941 cgtactttag tcatgttacg ccgatcatgt taatttgcag catgcatcag gcaggtcagg
    18001 gacttttgta cttcctgttt cgatttagtt ggcaatttag gtagcaaaca tgcagacccc
    18061 gcacattctt atcgttgaag acgagttggt aacacgcaac acgttgaaaa gtattttcga
    18121 agcggaaggc tatgatgttt tcgaagcgac agatggcgcg gaaatgcatc agatcctctc
    18181 tgaatatgac atcaacctgg tgatcatgga tatcaatctg ccgggtaaga acggtcttct
    18241 gttagcgcgt gaactgcgcg agcaggcgaa tgttgcgttg atgttcctga ctggccgtga
    18301 caacgaagtc gataaaattc tcggcctcga aatcggtgca gatgactaca tcaccaaacc
    18361 gttcaacccg cgtgaactga ctattcgtgc acgcaacctg ctgtcccgta ccatgaatct
    18421 gggtactgtc agcgaagaac gtcgtagcgt tgaaagctac aagttcaatg gttgggaact
    18481 ggacatcaac agccgttcgt tgatcggccc tgatggcgag cagtacaagc tgccgcgcag
    18541 cgagttccgc gccatgcttc acttctgtga aaacccaggc aaaattcagt cccgtgctga
    18601 actgctgaag aaaatgaccg gccgtgagct gaaaccgcac gaccgtactg tagacgtgac
    18661 gatccgccgt attcgtaaac atttcgaatc tacgccggat acgccggaaa tcatcgccac
    18721 cattcacggt gaaggttatc gcttctgcgg tgatctggaa gattaatcgg ctttaccacc
    18781 gtcaaaaaaa cggcgctttt tagcgccgtt tttatttttc aaccttattt ccagatacgt
    18841 aattcatcgt ccgttgtaac ttctttactg gctttcattt tcggcagtga aaacgcatac
    18901 cagtcgatat tacgggtcac aaacatcatg ccggccagcg ccaccaccag cacactggtt
    18961 cccaacaaca gcgcgctatc ggcagagttg agcagtcccc acatcacacc atccagcaac
    19021 aacagcgcga gggtaaacaa catgctgttg cgccagcctt tcaataccgc ctgcaaataa
    19081 ataccgttca ttaacgcccc aatcagactg gcgattatcc atgccacggt aaaaccggta
    19141 tgttcagaaa gcgccagcaa gagcaaataa aacatcacca atgaaagccc caccagcaaa
    19201 tattgcattg ggtgtaagcg ttgcgcggtg agcgtttcaa aaacaaagaa cgccataaaa
    19261 gtcagcgcaa tcagcagaat ggcatactta gtcgcccggt cagttaattg gtattgatcg
    19321 gctggcgtcg tcactgcgac gctaaacgcg gggaagtttt cccagccggt atcattgcct
    19381 gaagcaaaac gctcaccgag attatttgca aaccagctgc tttgccattg cgcctgaaaa
    19441 cctgactcgc taacttcccg tttggctggc aaaaaatcac ctaaaaaact gggatgcggc
    19501 cagttgctgg ttaaggtcat ttcgctatta cgcccggcag gcaccacaga aagatcgccg
    19561 gtaccgctta aattcagggc catattcagc ttcaggttct gcttgcgcca gtccccttcg
    19621 ggtaaaggga tatgcacgcc ctgcccgcct tgctctaacc cggtaccggg ttcgatggtc
    19681 agcgctgttc cgttaacttc aggcgctttc accacaccaa taccacgcgc atccccgacg
    19741 ctaatcacaa taaatggctt gcctaaggtg atatttggtg cgtcgagttc gctaagacgc
    19801 gaaacatcga aatcggcttt tagcgttaaa tcactgtgcc agacctgacc ggtataaatc
    19861 cctatcttgc gttcttcaac gttctgattg ccatcaacca tcaatgactc aggtaaccaa
    19921 aaatggataa aacttcgttt ccgctccacg gttttatcgt cttcctgcac cgtataaagc
    19981 tcggtcacag gaatagcgat gagcggccca acgagttttt gcggcccgct ggtactttgg
    20041 cgaatcgcat cttccacatc gctacggtaa tcggcacgtt cgacaatcac ctgccgaatc
    20101 agcattatcg gaatcaacaa cagcaatact gcaccaaaca aggtggtcat tttccagaac
    20161 aggggggatt tcaacatagt cttctccttt gcaatggcag caggatacga aggctatgtg
    20221 ggaagaattt gaagctatgt gaagtgacgg tgaagtcgta gcgaggccag cacgccacct
    20281 tcctgcacgt tgcgcagcgt gacttcgccg ttaaacaaac gggcgacctc actgacgaac
    20341 gccaacccca gaccgctgct tttttgccca tttgcacgcg gcaaagagta aaagcgttca
    20401 aaaatacgtg acagcgcgta gtcaggaata ccactaccgg tatccagcac cttaagggcg
    20461 acgtgttcct gatccacttc ggcgcttagc gttatgcgac cgctctcggg ggtaaaatcg
    20521 atggcgttat ccagtaaatt ccccagcgcc tgctccagta acgccggttc agcagcaaca
    20581 ttaacctcag tgggcataac atgtagagtg atgttttttt ctgccaactg cacggtgcgc
    20641 gcttcgctga cgcggcgaaa taatgccgcc acatcaacaa cagtcagaac gacttcctga
    20701 cgattctcca gtcttgcctg gcgtagtaac gtttctacca atgcctgcat acgcgcattt
    20761 tgcgtcagaa tgttgtcggt aaaacgagcc accacttccg gtggcggacc ttcgcgtaaa
    20821 atttccgccg cgccacgaat cgccgccagt gggcttttta gctcatgggt taacgcataa
    20881 acatactgct caatatagtt tttcccttcc agcttcacgc gcatactttc cagcgcctgc
    20941 gcaagcttac gcaactcgct actgccgagt tcaggaagag gaacgggctt attgtcagtg
    21001 acggaatcgg catagcgagt gagccgggca atggagcggt tgatccacca aaccatgcct
    21061 gcgccaatca ccagtgcaat ccccagcaaa atggcgctgg cccataaaat tcgccgctcg
    21121 ctacgcttaa tgaccggagc catcgccgcg ttcggtttgc ctacgctcaa aacgccaata
    21181 agccgcgagc cgtccataat cggcgcggca acatacatca ccgaactttc gggatcggca
    21241 ggattttgca acgtgctgcg cgcaccatac tgaccacgca acgttagcca gacgtcattc
    21301 cagcgcgaat aatcctgccc aacggcttta tttgccgaat cgaacaatac tttaccctgc
    21361 gaatcggtca tatagacatg atattcattg cgcactttgt taatgccacc gatattggcg
    21421 cgaaacgggc gatgttgtag ctgattaaac gcctgcgcca gttgcccatg cgttgggtcc
    21481 ccagagagca aatcgggacg cgccagctcc gccagcaacg ttgcggtatc gattaacgtc
    21541 ccctccgttg ctcttcgcac gcccggttta acttctttga caaaaatagc cagtacgaac
    21601 caggccgcca ccgccaccgc caccagtaaa aaatagccca gcagcaaccg catgccgata
    21661 cgcattacag gcccctcagg ctatatccca tgccgcgatg agtattaatc ggtgaaagat
    21721 cggggttgat ggcgcgcagc ttggcacgca acgttttaat gtgggtatcg acggtgcgat
    21781 cgtaggtgtc ctgcgcatct tcccacacgc tatccattag ttgctggcgg gaccagacgc
    21841 ggcctggcga cttgagtaac gtcttcagca ataaaaattc ataccgcgtc agcgttaatg
    21901 gcgtgtcaaa ccagctgatc tgcgccgcgg gttcattcaa ttcaaaatga ccaatacgga
    21961 tgacgggaga cggcgacgag aacttcttca cccgacgcag taaggtgcgc accctggcgc
    22021 acacttcgcg gggtgaaaac ggtttagcca catagtcgtc agcgccaatt tccagcccaa
    22081 gcaggcgatc gacctcttca cttcgggccg tcaggaacag tacaggtaac gccggatgga
    22141 gcgccagtaa ctggcggcac aattcaaagc cgctgatatc aggcaaacca acatcgagaa
    22201 tcatgacgtc gggaacctgc tggcgagctt tatccagcac cggcaagcct cgctcaaaga
    22261 cctcgacggc aaaaccttcc tgctgcaaca tgtagaccag cgtgtcggct atcccttgct
    22321 catcttccac taaccagacc gtttcccgtt gcataaatcg cctctgttat tgccgccacg
    22381 gcatgacagg aaccgcgcta atcgcgtttt tcggcgaacc ttctacaact ttgtcggagt
    22441 aagccagata agcgagcgcg ttgcgtttgg cgtcataaaa gcgcacgacc tgtaacgact
    22501 taaagaccag ggacgtgcgt tttttgaata ctacctcgcc ctgagctttg ccgtttttaa
    22561 tacgatccga cagttcaatc ggcccgactt gctgacaaga aatagccgca tcggaggtat
    22621 cttccgccag acccaatccc cctttaatac cacctgtttt cgcccggctc acataacagg
    22681 tgacattttt cacatcggga tcgtcaaagg cttccacaac aattttgtga tccgggccga
    22741 tcattttaaa tacggtgtcg accgaaccaa tctcttctgc atgagccaat ggccccagca
    22801 ttatcaggct taaagacagg atcaaatgct tgtatttcat attgttacca tttttatcta
    22861 ttacacgagc aattagttcg tcacggtaaa taacatattg attaagaaca aaatctcaat
    22921 acttttattt ccgtcaagcc ctaaaacata ctctactaag aaaaaaacac tgaatgctaa
    22981 aacagcaaaa aatgctatta tccaattacc tgatgtcagg tgctcgttgt tgaaaggatg
    23041 aggatatttt atggatcagg ccggcattat tcgcgacctt ttaatctggc tggaaggtca
    23101 tctggatcag cccctgtcgc tcgacaatgt agcggcgaaa gcaggttatt ccaagtggca
    23161 cttacagaga atgtttaaag atgtcactgg ccatgctatt ggcgcgtata ttcgtgctcg
    23221 tcgtttgtcg aaatcggcgg tcgcactacg cctgactgcg cgtccgattc tggacatcgc
    23281 gctgcaatac cgcttcgact ctcaacagac atttacccgc gcattcaaga agcagtttgc
    23341 ccagactcct gcactttacc gccgttctcc tgaatggagc gcctttggta ttcgcccgcc
    23401 gctacgtctg ggtgaattca caatgccaga gcacaaattt gtcaccctgg aagatacgcc
    23461 gctgattggt gttacccaga gctactcctg ttcgctggag caaatctctg atttccgcca
    23521 tgaaatgcgt tatcagttct ggcacgattt tctcggcaac gcgccgacca ttccgccagt
    23581 gctctacggc ctgaatgaaa cgcgtccgag tcaggataaa gacgacgaac aagaggtatt
    23641 ctataccacc gcgttagccc aggatcaggc agatggctat gtactgacgg ggcatccggt
    23701 gatgctgcag ggcggcgaat atgtgatgtt tacctatgaa ggtctgggaa ccggcgtgca
    23761 ggagtttatc ctgacagtat acggaacgtg catgccaatg ctcaacctga cgcgccgtaa
    23821 aggtcaggat attgagcgat actacccggc agaagacgcc aaagcgggag atcgcccaat
    23881 taatctgcgc tgtgaactgc tgattcctat ccgccgttaa cgctgcagct catctaatgc
    23941 aggggcgtcc agatgcgaga tgtcccctgc ggtttcaacg acccagccag acgccagcca
    24001 caggctttcc tgataatcca cgcgcgaaat agaacagtta cgcagacgta agcggcgctc
    24061 tgcccatgct ggtaatccga gaatcgtact caccaggcat cccagtgcaa taccatgact
    24121 caccagcagc ggtcggcttc cctgcggtaa gtcccggcaa gattccagtg ccgcattgac
    24181 gcgatcgctg agctcctgca ttgactcgcc ttcaggaata cgcccgtcaa cggtgccatt
    24241 gaccagctgc cgacgccagt tctcttcttc ttcggtcaga gaatcgatat gtcttttttc
    24301 cagcacaccc atgtttaatt cacgcaggcg agaatcaaag atgatgtcac agccgcaggc
    24361 ctgggcgatg atttccgccg tacgccgggt gcgtcctaaa tcgctactga taatatgcgt
    24421 aatgccaagc tctttggcac gggttgccac ctgcatcgcc tgttgctcac ctttggcggt
    24481 cagcgggctg tcagactggc cctgaatacg tcgctcggcg ttccactgcg tttcaccgtg
    24541 gcggactagg tatacctgta acatgctttt tttccgtata ctgctgtaac gttaattttc
    24601 tgagtaatgc tgattatgca ccaagttgtc tgtgcgacca ccaatcccgc taaaattcag
    24661 gccattctgc aggcatttca cgagatcttc ggcgaaggat cctgccatat tgcatccgtt
    24721 gccgtcgaga gcggtgtacc ggaacagccc tttggcagtg aggaaacgcg cgctggcgca
    24781 cgaaatcggg tagcaaatgc ccgccgttta cttccagagg ctgatttttg ggtggcgatt
    24841 gaagctggca tcgatggtga cagcactttc agttgggtgg tgattgaaaa caccagccag
    24901 cgcggcgagg cgcgttcggc tactttgccg ttacctgcgg tgattctgca gaaagtgcgt
    24961 gaaggtgagg cgcttggtcc cgtgatgtcg cgttataccg gtattgatga gattggtcgt
    25021 aaggaagggg cgatcggcgt gtttaccgcc gggaaactca ctcgcgccag cgtctatcac
    25081 caggcggtga tcctggcgct gagtccgttt cataatgccg tgtattaagc gcctgatgcg
    25141 acgctgccgc gtcttatcag gcctacaaaa tcaatcgctt ttcagcaaca cctcttccag
    25201 ccactggcgc agctccacgg gcgcggcttt caggctgtta gatccacgcg taatcgtcgc
    25261 gatgcccgcg ccgagttcat tttttaactc acgctgactc atttcgccac gcaacagctc
    25321 ttcgacaata cgcacgcgag tccccaacgc ttcgcgctca tctggcgtca gcatcaggtt
    25381 taacaacggt aaatggagat cgttttggta ggcattctta agcaggtcga caaaacgtaa
    25441 ccactcctgg tgacgctgtt ctgccatcgc tgctgaatag ggtgattgtt gggccataat
    25501 atgtcgccat tgttagcggg ggaagcaaaa tgcctccccc ggttgtactc gctaaagagt
    25561 acgatagcat atcataaacg tgcggatcag taacgacgtc cccattccgt ggcgctcatc
    25621 aacgtcggtt tatcccccat gaaatagcgg tagtaagcgt cataagccag cacgttcttc
    25681 acataaccgc gcgtctcgga gaacggaata ctctcgacaa atgccactgc gtcgatacgc
    25741 ccggcgctgt tgccaagcca ggttcgcacc cgccctggtc cggcgttata agctgctgag
    25801 gagaaaatac ggttattgcc aaactgctga taaacatatt gcagataact ggtgccaatg
    25861 ttgatattcg tttccggatc cagcaattgc ccagggctac tataaccggg aatagagaac
    25921 atcttcaccg tatgggtcgc tgtaccaggc ataatctgca tcaggccgct ggcccctacc
    25981 ggtgatttca cttttggatt ccaggcgctc tcctgacggg caatcgccat cgcatagctt
    26041 tgcgggatct ccttaccgct ggtgtagcgt ttgaaaagat cgttgtaagc cagcgggaat
    26101 cgctcttcca gatgatccca tagcttcccg gcgatcgttg cctgaacgct aagatcccac
    26161 cattggttgt tgaaagcata ccgcgccagt tgagcctgct ctgtttttga cttgctcttc
    26221 accagattgg cccactcgct acgcgcggtg ttatcgagat tccagtacat caactcgcgc
    26281 acgcgcgcca tctccggccc ctgagtcagg gcgctgtcaa cattctgcgg cgctttatca
    26341 atcttcagct catactcttc tccgatgcgt tgtgctgcaa ccatcgggta gaaaccacgc
    26401 tgttgcatga gttgatgcaa aatctctttc gcttcagctt cacgtccgcg ttccagcaat
    26461 aaatccgcct gccagtaacg ccattcatct ttctcttttg cttccatcgg cagacgcgcc
    26521 agccaggtat tcaggccgcg acgatcgccg gtgccaagtg ccattcgcac acggcgttca
    26581 atcaacgacg tggattgcga gcgcataatg gcgtcatcgc gccatttcgc ctgctcgtcg
    26641 gtgacatcgt tgcccatcaa acgccaggcg acgatatcgc gcagctcctg aatctgatct
    26701 tcattaagct gctgcgcctg ggcaagcgac gggatcatca gccgtgcatt ttcagcatcc
    26761 tgccgcgcca cactggcaaa cgccaccgct gccatttgac gggtaaaatc ggtcgctccg
    26821 gttgtacgcg cgaaggtcag taccgtatta gggttgttcg ccagtgaaat gattgccgag
    26881 gcgatagtct ggtaatcggc aggcatctgc cctgccagca ctgttaccag gccagtgtta
    26941 cccgctttca tcgccagacg gatacgctct aaatacgcca gcggatcttg tttacctgac
    27001 gcacgccaga cgctaaataa cttgtcacag gcgttaggct ggctcttgcc ggttagccac
    27061 agctctttcg ccccttgcca ggcttcttca ctctgcccgg tgttccattt cgcatagtag
    27121 taattacatt gcgcttcggt agttccgggc ttttccgggc taaaggctaa caagccacgc
    27181 cagtcttcac gccgcgccag ttcattgacg aaacgagatt gcagcgtgcg agcgggagga
    27241 agcgtggggt tagcgcgaac aaagttagtg accgtcaccg ccggttgatt catcagatca
    27301 tcggtgatct ggcggtattc caggtaggga taaagcggat aatccttcag tccaggcatc
    27361 atttgttcga ccacatccat ttgtcgatta tcccaggcct gcttgatttg cgcgtaacgg
    27421 ctacgctgct catccagtga gtcggctcgc gccacgctgc tgaccgtcag cagacagaca
    27481 ccggcagcca acagccgcca ggtaacttgt ttggcttttt ccacaagcgc atcctctaag
    27541 tgtaaataca tcaatgcagc gtcatgccgc gtaatagatt tatgctaacc agtcattgcc
    27601 gtttacgcca cgttacggac acttttttac ttttactgcg aaggtgatcg accaactggg
    27661 attaggcggg gaaaaaggct acacttcgcc tttgaaaact catcatcacg ataaacatag
    27721 aggcgaagtc caacgtggct caattcgttt ataccatgca tcgtgtcggc aaagttgttc
    27781 cgccgaaacg tcatattttg aaaaacatct ctctgagttt cttccctggg gcaaaaattg
    27841 gtgtcctggg tctgaacggc gcgggtaagt ctaccctgct gcgcattatg gcgggcattg
    27901 ataaagacat cgaaggtgaa gcacgtccgc agccagacat caagattggt tatctgccgc
    27961 aggaaccgca gctgaacccg gaacacaccg tgcgtgagtc cattgaagaa gcggtttctg
    28021 aagtggttaa cgccctgaaa cgcctggatg aagtgtatgc gctgtacgcc gatccggatg
    28081 ccgattttga caagctggcc gctgaacaag gccgtctgga agagatcatt caggctcacg
    28141 acggtcataa cctgaacgta cagctggagc gtgcggcgga tgcgctacgt ctgccggact
    28201 gggacgcgaa aatcgctaac ctctccggtg gtgagcgtcg tcgcgtagcg ttgtgccgcc
    28261 tgctgctgga aaaaccagac atgctgctgc tcgacgaacc gaccaaccac ctggatgccg
    28321 aatccgtggc ctggctggaa cgcttcctgc acgacttcga gggcaccgtg gtggcgatta
    28381 cccacgaccg ttacttcctc gataacgttg caggctggat cctcgaactt gaccgcggtg
    28441 aaggtattcc gtgggaaggc aactactcct cctggctgga gcagaaagat cagcgcctgg
    28501 cgcaggaagc ttcacaagaa gcggcgcgtc gtaagtcgat cgagaaagag ctggagtggg
    28561 tacgtcaggg aactaaaggc cgccagtcga aaggtaaagc acgtctggca cgctttgaag
    28621 agctgaacag caccgaatat cagaaacgta acgaaaccaa cgaactgttt attccacctg
    28681 gaccgcgtct gggcgataaa gtgctggaag tcagcaacct gcgtaaatcc tacggtgatc
    28741 gcctgctgat tgatgacctg agcttctcga tcccgaaagg ggcaatcgtc gggatcatcg
    28801 gtccgaacgg cgcgggtaaa tcgaccctgt tccgtatgat ctctggtcag gaacagccgg
    28861 acagcggcac catcacttta ggtgaaacgg tgaaactggc atcggttgat cagttccgtg
    28921 actcaatgga taacagcaaa accgtttggg aagaagtttc cggcgggctg gatattatga
    28981 agatcggcaa caccgagatg ccaagccgcg cctacgttgg ccgctttaac tttaaagggg
    29041 ttgatcaggg taaacgcgtt ggtgaacttt ccggtggtga gcgcggtcgt ctgcatctgg
    29101 cgaagctgct gcaggttggc ggcaacatgc tgctgctcga cgaaccaacc aacgacctgg
    29161 atatcgaaac cctgcgcgcg ctggaaaacg ccctgctgga gttcccgggc tgcgcgatgg
    29221 ttatctcgca cgaccgttgg ttcctcgacc gtatcgccac gcacattctg gattaccagg
    29281 atgaaggtaa agttgagttc ttcgaaggta actttaccga gtacgaagag tacaagaaac
    29341 gcacgctggg cgcagacgca ctggagccga agcgtatcaa gtacaagcgt attgcgaagt
    29401 aatgcgtaaa atgccggatg cggcgtgaac gccttatccg gcctacaaac caagaaattt
    29461 caatacgttg tgaatctcca ggcctgataa gacgcggtaa gcgtcgcatc aggcattgag
    29521 caccgaatgc cggatacggc gtgaacgcct tttccggcct acaacagcct tagtatccgc
    29581 aaattcgtag cgggtcaccc gtgctgattc ttttaaacct tgtttaatct cgacagcctc
    29641 ttccagagag gttttcaact catcaaaaaa cttattttat acctcgcctt tgagccgttt
    29701 catcgcggtt atctctgctc ccccatcatc tcccgcacca gctccacgca gcgcaggaaa
    29761 cggctgtcat aatcgtcctc ttcaacccgc acgaattcga tattgttctc ttccagcatc
    29821 tccaccagca agttctggaa ctctttgcga tccaccgaac tgccgaggct gcgtaaacca
    29881 tccgccaccc acggcgtgtt gttctccagc aggatcacca gatcgaaacg gtattcatca
    29941 atcaacgcct gtacgaacgg atgctcacgc ccttcgtact ttttgcagaa cgcctgggta
    30001 gtgacaaaat cagtgtcaat aaacgccact ttattggcat atttcactgc aaaatcaatg
    30061 tattgtgcgt ggcccagcgc gattttatcg taatcggaat actgcaatgc gatctcatca
    30121 ccgccgaggt gtgaaaagac ataatcgcga ccatattccc acgcactggt ggtattgaag
    30181 atattggcaa gtttgtttac cagggtggat ttaccgctcg actcgccacc aaggatcgcc
    30241 acggtacgta caaagaacgg cttcacttcc gtaggaatat attcccagta gcggaacggg
    30301 ttttcgcgga tctgcgcacc gctgatactc ataaaggtac gtttcggatc gaccagcacc
    30361 gtatcgatcc ccagatgttc catatactgt ggcgcatcgg cttcttccga ggtgtagatc
    30421 aggtccggct gaatcccctt ttcagccata aactttttga tgccgttgct ccacacatcc
    30481 cagccgtgcg gatacggctc catgccctct tcgttgaaag catgaatgcg aatatttttc
    30541 tgatatttaa aagtttgtaa taaccagcgc agacgatccg gcaccgtggg ctgctgcgac
    30601 atggcgctgt cttcaaacaa cgcgcggtcg cgggtatcgt caaaacccat aatgatatgc
    30661 agctcgtcaa cctggctaca ggcgcgctgg ataaggtaga tatgtccggt atgcagcggg
    30721 tagaacttac cgaatacgac accaatggtt ttcttctgtc ggggaaattc aagccccaaa
    30781 aaacggtgca acgcctccag cttttgcgcg ctggggcttt taattttggc attcagtaac
    30841 tggcttaaat accctttggt cataccgctg gcatcagcta cctgctgtag cgtgcagccc
    30901 tgttgcttga tggcagtttt caggtaatca aatgacgaca tatctccctc cgtatctctc
    30961 attataagtc gtcgaacacg ctaagcgcgt cggagagttt tttaacgcca aaaatctgca
    31021 tcccttccgg cgcttttttc ggtacgttag ccgccggaac aatcgcccgg cgaaaaccgt
    31081 gtttcgccgc ttctgaaatt cgttcctgac cgctgggcac cgggcggatc tcccctgcca
    31141 gcccgacttc accaaacacc accagatcct gtggcagcgg tctgtcacgc aggctggaaa
    31201 ccatcgccag cagtaacgct aaatcggcac tggtttcggt taccttcacg ccgccgacca
    31261 cgttcacaaa tacatcctga tcggccattt gcagaccacc gtgacggtgc aagacagcca
    31321 gcaggattgc cagacggttt tgttccagcc ccactgccac gcggcgcgga ttcgccatca
    31381 tcgagtgatc gaccagcgcc tgaatctcca ccagcaacgg acgcgttcct tcccacacca
    31441 ccatcacgga gctgccggag gtcacttcat ctccgcgact taagaaaatt gccgaagggt
    31501 tgctgacttc acgcagcccc tgttcggtca tcgcgaagac gcccagctca ttcaccgcgc
    31561 cgaagcggtt tttatggctg cgcaaggtgc gaaaacggga gtcggcatca ccatccagaa
    31621 gcaccgaaca gtcgatacag tgttccagca ctttagggcc agccagcgag ccatctttgg
    31681 ttacgtgccc aaccatgaca atcgccacac cgcgcgtttt ggcgaagcgc gtcagataag
    31741 ccgccgtttc acgcacctgc gccacgctac caggcgatga ttgtacatcc gccatatgca
    31801 tcacctggat cgagtcaatt accatcagtt tcggttgctc ttcttcggca atcaggcaga
    31861 tctgttcgat gctggtttcc gacaacatat tgagattgtc agtcggcagg ccaaggcgat
    31921 gagcgcgcat tgccacctgt tgcagcgact cttcgccagt gacatacagc gttttcatct
    31981 gctgggccag tttgcacagc gtttgcagta gcagcgtgga tttccccgca cctgggttac
    32041 cgccaatcag aatggcgctt cctggcacca cgccgccgcc tagtacgcgg tcgaactctt
    32101 taaatccggt ggaaaaacgc ggcagctctt caaggctgat atcggagagt ttctggactt
    32161 ttgccacccc ggcgctaccg gcatagccgc tgagacgctc gttacgcgcc accgttggcg
    32221 acgcagcaag acgcacctcg gtgatggtgt tccaggcatg acaggcactg cactgcccct
    32281 gccagcgcgg ataatcggcc ccgcattcat tacaaacaaa ggcgcgtttt ggagcttttg
    32341 ccacggtgta cctcgttaat gctgtgccgc ccgcaggatg acgggcgagc aattacttct
    32401 gattcaggct gcctgagagg atgcagaata cccccatcag gtcagcgtga cggatggtga
    32461 cttccgtctt ttcattcact tttggcttgg catggtaggc aatccccagc cctgctgctt
    32521 tgatcatcgg caggtcattg gctccatcgc caatcgccac ggtctgcgcc agcggaattt
    32581 catactcttg cgcgaggcgg gtcagagttt tcgctttgta ctgcgcgtca acgatgtcgc
    32641 cgatcacatt gccggtaaat ttaccgtcca tgatctccag ttcattggct accacggcgg
    32701 tcaggcgcag cttgtcgcgc aggtattcag caaagaaagt aaagccgccg gaggcaatcg
    32761 ccactttcca gcccagcgtt tccagcttga gcaccagttg cgttaagcct ggcatcagcg
    32821 gcagattttc acgcacctgt tgcagaatat tggcgtcagc gcctttcagc gtcgccacac
    32881 ggctgcgcag gctggcggta aaatcgagtt cgccgcgcat cgcccgttcg gttacttccg
    32941 ccaccatctc gcccgttccg gccagtttgg caatttcatc aatacattca atctggatgg
    33001 cggtggaatc catgtccatc accagcaaac ccggcgtgcg caggtgcggg attttcccca
    33061 gcggggcgac atccagttgc gcttcatggg ccaggcgcgt agcccgtgcg gtgagtgaac
    33121 ctgccagacg aatcacctga taatcttcca cgcaccaggc ggcaacaatc accatcgccg
    33181 cacccagttt gctctggtat tgggtcagac gctgtttatc cagcccacga ccatacagca
    33241 gccagccgct acgacctgcg tggtaatcca gtggcatcac ttcatcacca cttaatgaaa
    33301 gaggcagacc cggccataaa gagacatctt caggcaggtc gcaccaggta atgttaggca
    33361 ttaaggctcc tgtaaaatcg ttcgaagcag ggaaaataac gcatgaggct accttgtatc
    33421 cattgcttct ggcaacatta agtctcaaat tttcaaaggg tggaagatgg ctcgcacaaa
    33481 actgaaattc cggctgcatc gggcagtgat tgtcctgttc tgtcttgcct tgttagtggc
    33541 actgatgcag ggggcgtcat ggtttagtca aaaccaccag cgacagcgta atccacagct
    33601 ggaagaactg gcccgcaccc tggcgcgtca ggtgacgctg aacgttgcac cgctgatgcg
    33661 taccgactca ccagatgaaa aacgcattca ggcgatcctc gatcagttaa cggatgaaag
    33721 ccgcatcctc gacgcgggtg tgtatgacga acaaggcgat cttatcgccc gttcaggcga
    33781 aagcgtcgaa gtgcgcgacc ggctggcgct cgacggtaaa aaagcaggcg gctattttaa
    33841 ccagcagatt gtcgagccaa ttgcgggtaa aaacggaccg ctcggctatc tgcgcctgac
    33901 gctcgacacc catacgctcg ccaccgaagc ccaacaggtg gataacacca ctaacatttt
    33961 acgcctgatg ttgctgctct cactggcaat cggcgtagtg ctgacccgca cgctgctaca
    34021 gggtaaacgc acccgctggc agcaatcgcc cttcctgtta accgccagca aaccggtgcc
    34081 ggaagaggaa gaaagcgaga aaaaagagtg acccattact acaagaaagg aaatcgttat
    34141 gtccacatta cgcctgctca tctctgactc ttacgacccg tggtttaacc tggcggtgga
    34201 agagtgtatt tttcgccaaa tgcccgccac gcagcgcgtt ctgtttctct ggcgcaatgc
    34261 cgacacggta gtgattggtc gcgcgcagaa cccgtggaaa gagtgtaata cccggcggat
    34321 ggaagaagat aacgtccgcc tggcgcggcg cagtagcggt ggcggcgcgg tgttccacga
    34381 tctcggcaat acctgcttta cctttatggc tggcaagccg gagtacgata aaaccatctc
    34441 tacgtcgatt gtgctcaatg cgctgaacgc gctcggcgtc agcgccgaag cgtccggacg
    34501 taacgatctg gtggtgaaaa cagtcgaagg cgaccgcaaa gtctcaggct cggcctatcg
    34561 cgaaaccaaa gatcgtggct tccaccacgg cactttgctg ctcaatgccg accttagccg
    34621 cctggcaaac tatctcaatc cggataaaaa gaaactggcg gcgaaaggca ttacctcagt
    34681 gcgttcccgc gtgaccaacc tcaccgagct gctgccgggg atcacccatg agcaggtttg
    34741 cgaggccata accaaggcct ttttcgccca ttatggcgag cgtgtagaag cggaaatcat
    34801 ctccccggac aaaacgccag acttgccaaa cttcgccgaa acctttgccc gtcagagtag
    34861 ctgggaatgg aacttcggtc aggctccggc attctcgcat ctgctggatg aacgctttag
    34921 ctggggcggc gtggaactgc atttcgacgt tgaaaaaggc catatcaccc gcgcccaggt
    34981 gtttaccgac agcctcaacc ccgcgccgct ggaagccctc gccgggcgac tgcaaggctg
    35041 cctgtaccgc gcggatatgc tgcaacaaga gtgcgaagcg ctgttggttg acttcccgga
    35101 ccaggaaaaa gagctacggg agttgtcgac gtggatagcg ggggcggtaa ggtaactcgc
    35161 tgattaaaat accaaaggaa gggaatatga tcgatctgga aaaccaggag cgggagatta
    35221 tcaatttaat gttaagccag cgtatttcct ggctagccgc cgtgcgtatc aggcataaat
    35281 tatcgcttgc tgaagtaagt aaaatgttag gtatctcgat taattctcta aaatagatcg
    35341 aaaagacgga acggtttagc tcaaacatca aaagcaaaat ggcagaaatt tatggttgtc
    35401 caccagagtt gctgatatgc ccttcctgga tgacggctga acataaatga atagattcgt
    35461 gaatagatac ttgaatagat tttattttct atccatatg
//
LOCUS       DAJOWB010000037        35181 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJOWB010000037 DAJOWB010000000
VERSION     DAJOWB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.037"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(102..1286)
                     /locus_tag="OHO85_002002"
     CDS             complement(102..1286)
                     /locus_tag="OHO85_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2671378.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(1586..1680)
                     /locus_tag="OHO85_002003"
     tRNA            complement(1586..1680)
                     /locus_tag="OHO85_002003"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1644..1646),aa:Sec,seq:tca)
     gene            1973..3355
                     /locus_tag="OHO85_002004"
     CDS             1973..3355
                     /locus_tag="OHO85_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCQ2671379.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3365..5683
                     /gene="yicI"
                     /locus_tag="OHO85_002005"
     CDS             3365..5683
                     /gene="yicI"
                     /locus_tag="OHO85_002005"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCQ2671380.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPATDGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(5736..7445)
                     /locus_tag="OHO85_002006"
     CDS             complement(5736..7445)
                     /locus_tag="OHO85_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCQ2671381.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPKL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(7566..8957)
                     /gene="xanP"
                     /locus_tag="OHO85_002007"
     CDS             complement(7566..8957)
                     /gene="xanP"
                     /locus_tag="OHO85_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCQ2671382.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVLSRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            9237..10442
                     /gene="gltS"
                     /locus_tag="OHO85_002008"
     CDS             9237..10442
                     /gene="gltS"
                     /locus_tag="OHO85_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCQ2671383.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            10445..11311
                     /locus_tag="OHO85_002009"
     CDS             10445..11311
                     /locus_tag="OHO85_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2671384.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(11296..13377)
                     /gene="recG"
                     /locus_tag="OHO85_002010"
     CDS             complement(11296..13377)
                     /gene="recG"
                     /locus_tag="OHO85_002010"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCQ2671385.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELQLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(13383..14072)
                     /gene="trmH"
                     /locus_tag="OHO85_002011"
     CDS             complement(13383..14072)
                     /gene="trmH"
                     /locus_tag="OHO85_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCQ2671386.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(14079..16187)
                     /gene="spoT"
                     /locus_tag="OHO85_002012"
     CDS             complement(14079..16187)
                     /gene="spoT"
                     /locus_tag="OHO85_002012"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCQ2671387.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(16206..16481)
                     /gene="rpoZ"
                     /locus_tag="OHO85_002013"
     CDS             complement(16206..16481)
                     /gene="rpoZ"
                     /locus_tag="OHO85_002013"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCQ2671388.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(16536..17159)
                     /gene="gmk"
                     /locus_tag="OHO85_002014"
     CDS             complement(16536..17159)
                     /gene="gmk"
                     /locus_tag="OHO85_002014"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCQ2671389.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17417..19099
                     /gene="ligB"
                     /locus_tag="OHO85_002015"
     CDS             17417..19099
                     /gene="ligB"
                     /locus_tag="OHO85_002015"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCQ2671390.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAELPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGNWLAAQQITGFEP"
     gene            complement(19096..19713)
                     /locus_tag="OHO85_002016"
     CDS             complement(19096..19713)
                     /locus_tag="OHO85_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCQ2671391.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(20004..20606)
                     /gene="dinD"
                     /locus_tag="OHO85_002017"
     CDS             complement(20004..20606)
                     /gene="dinD"
                     /locus_tag="OHO85_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCQ2671392.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQANTTHFD
                     VGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(20827..21690)
                     /locus_tag="OHO85_002018"
     CDS             complement(20827..21690)
                     /locus_tag="OHO85_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCQ2671393.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            21817..22533
                     /gene="rph"
                     /locus_tag="OHO85_002019"
     CDS             21817..22533
                     /gene="rph"
                     /locus_tag="OHO85_002019"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCQ2671394.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            22599..23240
                     /gene="pyrE"
                     /locus_tag="OHO85_002020"
     CDS             22599..23240
                     /gene="pyrE"
                     /locus_tag="OHO85_002020"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCQ2671395.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            23538..24497
                     /locus_tag="OHO85_002021"
     CDS             23538..24497
                     /locus_tag="OHO85_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HCQ2671396.1"
                     /translation="MDATRTTLLALDLFGSPGWSADKEIQRLHALSNHAGRHYRRIIL
                     SKRHGGQRLVLAPDYLLKTVQRNILKNVLSQFPLSPFATAYRPGCPIVSNAQPHCQQP
                     QILKLDIENFFDSISWLQVWRVFRQAQLPRNVVTMLTWICCYNDALPQGAPTSPAISN
                     LVMRRFDERIGEWCQARGITYTRYCDDMTFSGHFNARQVKNKVCGLLAELGLSLNKRK
                     GCLIAACKRQQVTGIVVNHKPQLAREARRALRQEVHLCQKYGVISHLSHRGELDPSGD
                     LHAQATAYLYALQGRINWLLQINPEDEAFQQARESVKRMLVAW"
     gene            complement(24543..25139)
                     /gene="slmA"
                     /locus_tag="OHO85_002022"
     CDS             complement(24543..25139)
                     /gene="slmA"
                     /locus_tag="OHO85_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCQ2671397.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(25246..25704)
                     /gene="dut"
                     /locus_tag="OHO85_002023"
     CDS             complement(25246..25704)
                     /gene="dut"
                     /locus_tag="OHO85_002023"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCQ2671398.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(25682..26902)
                     /gene="coaBC"
                     /locus_tag="OHO85_002024"
     CDS             complement(25682..26902)
                     /gene="coaBC"
                     /locus_tag="OHO85_002024"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCQ2671399.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPARVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDEFTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            27074..27742
                     /gene="radC"
                     /locus_tag="OHO85_002025"
     CDS             27074..27742
                     /gene="radC"
                     /locus_tag="OHO85_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCQ2671400.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            27959..28195
                     /gene="rpmB"
                     /locus_tag="OHO85_002026"
     CDS             27959..28195
                     /gene="rpmB"
                     /locus_tag="OHO85_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCQ2671401.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            28216..28383
                     /gene="rpmG"
                     /locus_tag="OHO85_002027"
     CDS             28216..28383
                     /gene="rpmG"
                     /locus_tag="OHO85_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCQ2671402.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            28481..29290
                     /gene="mutM"
                     /locus_tag="OHO85_002028"
     CDS             28481..29290
                     /gene="mutM"
                     /locus_tag="OHO85_002028"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCQ2671403.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(29329..29808)
                     /gene="coaD"
                     /locus_tag="OHO85_002029"
     CDS             complement(29329..29808)
                     /gene="coaD"
                     /locus_tag="OHO85_002029"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCQ2671404.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(29816..31093)
                     /gene="waaA"
                     /locus_tag="OHO85_002030"
     CDS             complement(29816..31093)
                     /gene="waaA"
                     /locus_tag="OHO85_002030"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCQ2671405.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            31506..32564
                     /gene="rfaQ"
                     /locus_tag="OHO85_002031"
     CDS             31506..32564
                     /gene="rfaQ"
                     /locus_tag="OHO85_002031"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCQ2671406.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            32561..33685
                     /locus_tag="OHO85_002032"
     CDS             32561..33685
                     /locus_tag="OHO85_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCQ2671407.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            33678..34475
                     /gene="rfaP"
                     /locus_tag="OHO85_002033"
     CDS             33678..34475
                     /gene="rfaP"
                     /locus_tag="OHO85_002033"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCQ2671408.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            34491..>35181
                     /locus_tag="OHO85_002034"
     CDS             34491..>35181
                     /locus_tag="OHO85_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,3-galactosyltransferase"
                     /protein_id="HCQ2671409.1"
                     /translation="MSAHYFNPQEMITKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKVLYLDADIACQGNLKPLITMDLANNIAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSG"
BASE COUNT         8334 a         8661 c         9357 g         8829 t
ORIGIN      
        1 agacgcaaaa aaaataatat aataacaaaa atcctccagc cttgtaggcc agaggataaa
       61 atacttaatt gaggaaaata tcagggaaaa gtatttctga tttaactttc ccggctggag
      121 agtgaatcaa tgtaatcagc gtaccattgc atcatttcac ggcggccatc gagatacagg
      181 gcatgattat aggtacccct gatggaattt ttatccacat gagctaactg catttcaatc
      241 caggcggaat taaagccttg ttcatgcaga attgtactca tggtgtgtct gaaaccgtgc
      301 cctgtgagtc ttcccttata tccaagtaat tcaattactt ggttaatgct ttccttacta
      361 attggtttgc ggggatcatt tctaccgata aagactaggg gatgatgttt tgagattggt
      421 tcaagctgct taaatagcat aatgacctgc tcagagagcg gcacaatatg aggtcgcttc
      481 atcttcatta cttctgccgg gatctcccac aattttgtct caaaatcgat atcttcccag
      541 cgtgcaaaac gcagttcttg tgttcgtacg ccggttaaca taatgatctg agtagctgtc
      601 ttagtgatta tacttccggt ataacccgcc agatcgttga ggaagtgggg aagttcatta
      661 gcagtaagaa agggaaaatg tacttttttc ggtgtagcta aagcactggc aagatcgggg
      721 gctgggttat agtctgcccg gccagtaaca attgcatagc ggaacacctc accacagcgc
      781 tggcggacct tgcgcatttt ctccagagct ccacgttttt ccattttacg cagtgcttcc
      841 agtaactcca ttggtttgat ttcggcaata gggcgcttgc caatgtaggg aaaaatgtct
      901 ttttcaaagg tatcaatgat ttcatcacga tagcgtaatg accaccgatc agcttttgac
      961 tggtgccatt cgcgagcaat agcctcgaac gtatttttca gactcatttg ctgcgcgatt
     1021 ttttcttcct tttttgcctc ccctggatcg cctccagcag cgaggagttt cctggcttca
     1081 ctgcgtttct cgcgagcatc ggctaacgtt acatcggggt atacgccaat agaaagcatc
     1141 ttttctttac cagcaaagcg gtacttcatg cgccagtatt tagaaccatt agtgttaatc
     1201 agtagataca taccgccgcc atcagcaagc ttatagggtt tgtctttggg ttttgcggtt
     1261 ttcactttga tatcggttaa tgccatgaca tcctccggct tttgatgaat tcatcttcct
     1321 gagggggatt tctgccagta actaacctga tacccccaat acaccccttg tataacaaag
     1381 atattgttaa aagccgttgt tatgtatcat atttttccta ttatgggggt gcttgtattt
     1441 caataggaaa taacaccccc taaagtaccc ccaaaagaaa cttgatatgg ggtgaagtag
     1501 gttgacctca ggatacaaat aaatactgat atacaaggga ttagaagaga tttattgaca
     1561 ttgggagagt tcagaagagg taaactggcg gaagatcaca ggagtcgaac ctgcccggga
     1621 ccgctggcgg ccccaactgg atttgaagtc cagccgcctc accggagacg acgatcttcc
     1681 gcgcctcgat tgctacatgg aggcggggcg cattatagct acttccttga gtttctacat
     1741 cccccagatc agttttttcc gcctaataca cgcagcgtat tatcacgaaa gcattatagt
     1801 tcatataaaa cccatatttt acaatgatat atgagcggaa ttaacgctac cacgatcaca
     1861 taaaacaaga atcataaatt aataaccaga tatcggaata ttcgctctca cagggatggt
     1921 ttacaaaatg cgttatcggt ctcagcaccc ctatagcatc aaggaaaagc agatgaagag
     1981 tgaagtgttg tccgttaaag agaaaattgg ttatggcatg ggagacgccg ccagccacat
     2041 tattttcgat aacgtaatgt tatatatgat gttcttttat accgatattt ttggcattcc
     2101 tgccggtttt gtcggaacca tgtttttggt cgctcgtgca ctggatgcga tttccgatcc
     2161 ttgcatgggg ttgttggccg atcgaacgcg ctctcgctgg ggtaaatttc gtccgtgggt
     2221 actgtttggc gcactgccat tcgggatcgt ctgtgtactg gcctatagca cgccagatct
     2281 cagtatgaac ggcaaaatga tctatgcagc aattacttac accctactta ccttacttta
     2341 taccgtcgtc aatatccctt actgcgcatt gggtggtgta atcaccaatg acccgactca
     2401 gcgtatctcg ctgcaatcct ggcgttttgt gctggcgacc gcgggaggca tgctttctac
     2461 tgttctgatg atgccactgg ttaatttaat tggcggtgat aataaaccac tcggtttcca
     2521 gggcggtatc gcggtccttt ccgtggtggc attcatgatg ctggcatttt gtttcttcac
     2581 cactaaagaa cgcgttgaag caccacctac aacaacgtct atgcgggaag atttacgtga
     2641 tatctggcaa aacgaccagt ggcggattgt cggtttacta accattttca atatcctggc
     2701 ggtgtgcgta cgcggtgggg cgatgatgta ttacgtcaca tggattttgg gcacgccgga
     2761 agtgtttgtc gcttttctca ccacttattg cgtgggtaac ctgattggtt ccgcactggc
     2821 aaaacctctg accgactgga aatgtaaagt cactatcttc tggtggacga acgccctgct
     2881 ggcagtgatt agcctcgcga tgttctttgt tcccatgcag gccagcatca ctatgtttgt
     2941 cttcatcttc gtgattggtg tgttgcatca actggtgaca cctatccagt gggtaatgat
     3001 gtccgatacc gtcgactacg gcgagtggtg caatggtaaa cgcctgaccg ggatcagttt
     3061 tgctggcacg ctgtttgtgc tcaaactggg gttggccttc ggcggcgctc ttatcggctg
     3121 gatgctggct tatggcggat atgatgcggc agaaaaagcg cagaacagcg ccacgattag
     3181 catcattatt gcgctattca cgattgttcc ggcgatctgt tatttgctga gcgcgattat
     3241 cgctaaacgc tactactcac tcacgacgca caatctgaaa accgttatgg aacagctggc
     3301 tcagggtaaa cgccgttgcc agcaacaatt cacctctcaa gaagtgcaga actaaggaac
     3361 ggcaatgaaa attagcgatg gaaactggtt gattcaacct ggcctcaatt tgattcaccc
     3421 gcttcaggtg ttcgaggttg aacagcagga taatgaaatg gtggtctatg ctgccccccg
     3481 tgatgtgcgt gaacgtacct ggcagcttga tacgccttta tttacgttgc gctttttctc
     3541 cccacaggaa ggtattgtcg gtgtgcggat tgagcatttt cagggggcgc tgaataacgg
     3601 tcctcattat ccgctcaata ttttgcagga cgtgaaggtc acaatcgaaa acacagaacg
     3661 ttatgctgag tttaaaagtg gcaacttaag cgcgcgtgtc agcaaaggtg agttctggtc
     3721 actggatttt ctgcgcaacg gcgaacgtat taccggtagt caggtgaaaa ataatggcta
     3781 cgtgcaggac acgaataatc aacgcaatta tatgtttgag cggcttgatc ttggcgttgg
     3841 cgaaacagtt tacggtctgg gagagcgctt tactgccctg gtgcgcaatg gccagacggt
     3901 agagacctgg aaccgggacg gcggcacaag tactgaacag gcgtataaaa atatcccgtt
     3961 ctacatgact aaccgtggtt atggggtact ggtcaatcat ccccagtgtg tctcttttga
     4021 agtgggatcg gagaaagtct ccaaagtgca gttcagcgtt gagagtgaat atctcgaata
     4081 ctttgttatc gacggcccga cgccgaaagc ggtacttgat cgttataccc gctttactgg
     4141 tcgtccggcg ctgccgcccg cgtggtcctt cggcctgtgg ctaaccactt catttaccac
     4201 caactacgac gaagcgacgg taaacagctt tatcgatggt atggcggaac gcaatctgcc
     4261 gctgcatgtt ttccactttg actgtttctg gatgaaagcc ttccagtggt gcgattttga
     4321 gtgggacccg ctgactttcc ctgacccgga agggatgatc cgccgcctga aagcgaaagg
     4381 gctgaaaatc tgcgtctgga ttaaccccta tatcggtcaa aaatcccccg tctttaaaga
     4441 gttacaagag aaaggctatt tactcaaacg ccccgacggt tcgctatggc agtgggataa
     4501 atggcagcca ggtctggcga tttatgactt taccaatccg gatgcctgca aatggtacgc
     4561 cgacaaactg aaaggtctgg tcgcgatggg cgttgattgc tttaagaccg actttggcga
     4621 acgtatccca actgatgttc agtggtttga cggttccgat ccgcagaaaa tgcataacca
     4681 ttatgcgtac atctacaacg aactggtgtg gaacgtgctc aaggacaccg ttggtgagga
     4741 agaagctgtc ttgtttgccc gctcggcctc cgtcggtgcg cagaaattcc cggtacactg
     4801 gggtggcgat tgttacgcta actacgaatc aatggcggaa agcctgcgcg gtggtttgtc
     4861 tattggcctt tcaggttttg gcttctggag ccacgatatc ggcggctttg aaaataccgc
     4921 tccggcgcac gtttacaaac gctggtgcgc gtttggtttg ctctccagcc atagccgttt
     4981 acacggtagc aaatcttatc gtgtgccgtg ggcctacgat gatgagtcct gtgatgtggt
     5041 gcgcttcttc acgcaactga aatgccgcat gatgccgtat ctgtatcgtg aagctgcgcg
     5101 tgcgaacgcg cggggtacgc cgatgatgcg ggccatgatg atggagttcc cggacgatcc
     5161 ggcttgtgat taccttgacc gtcaatacat gttaggcgac aacgtgatgg ttgcgccggt
     5221 gttcactgaa gcgggcgatg tgcagttcta cctgccggaa ggtcgctgga cacacctgtg
     5281 gcacaacgat gaactcgacg gtagtcgctg gcataaacag cagcacggct tcctgagtct
     5341 gcccgtttat gtgcgtgata acactctact ggcgctgggc aacaacgatc aacgtcccga
     5401 ttacgtgtgg cacgaaggca cggcattcca cctctttaat ctgcaagacg ggcatgaagc
     5461 cgtctgtgaa gtgcccgcta ctgacggatc ggtgatcttt actttaaaag cagcacgtac
     5521 tggcaacacg attactgtga ctggtgcggg cgaggcgaag aactggacgc tgtgcctgcg
     5581 caatgttgtg aaagtaaatg gtctgcaaga cggttcgcag gctgaaagtg agcaggggct
     5641 ggtggtgaag cctcaaggga atgcgctgac aattacgttg taatctattg atggaatgcc
     5701 gggtacgtct gagtcgtacc cggcgagatt gatgtttacc gcaccggctc cgtcgatgta
     5761 acttcaccgc tggaaacgat gccagcattc atggtttgag tcacttgctg ctttgcggcg
     5821 ttcacggcat gcaattgccc gctaaccgta ggtttcaacg ggacattggc ctgaatatcg
     5881 ccactggcgg taagctgaat attgccgtcg ccagtcaacg gtaatttagg ccatccccac
     5941 tgttgcaaaa tattcaccgg cacaccgcgt ccattcaggc tgatatgtgt ctgacgttgt
     6001 ggcgtttgtg aaacactggc ggtggcttcc agaatgcctt tttcagtaaa tgcactcagt
     6061 tcgctgatat tcaccgtgct gctgttggcg gtcagcgcca gcgacgggcg acgaacatca
     6121 acacgattga atgtcgcggc ggcggcattc agattcgccg agccactcca gacgccccat
     6181 ttatgatcgg taaccagcgt caggttggca ccgtaaccat cgagcgtggt gagctgccac
     6241 gggaagtcag ggtcgatatc aatgatcaga ttgcggctgg cgctaaatct cttcagttgc
     6301 aggctgttta accaaccggg tgtcgtttcc atccacaact gttgccagtt tttcggcaag
     6361 gtatattcca gcccggcaat tgccgcatca tcaaggatca acgttttccc gtcacgcagc
     6421 cagttccctg acgttctgac cataccccct tcccagcggc tggtgaactg gcgcagcgct
     6481 acgccctgcg gggaaaattc cgcgtttata atcgggtcaa ataaatgcag cgaaccataa
     6541 atgaactcgc tagcgttcat cgacagtttg ccttcttgtg tctgccagtc atctttactg
     6601 aaggtcatgt tgcgcaagct gagatcgagg tcggtcaccg cccagtccgg accttgcaaa
     6661 cgagcatcga tcacttccag gcgaccaatc tgcaacgacg ggacagagcg taatggcgca
     6721 aagaagtcgg ttagcgattt ttcgctttgt agacgtatat ccgccatgcg caggttttcc
     6781 acttgccagc tgccgtcggc gttacgctgc gcgtttccgg ttaatgtccc gcgggcgatg
     6841 tcggcaccca ggttagtcag cgtaacgcga tcattatcaa tactgccttc aatcagtaca
     6901 ttggtggcag gaacatcatt gagcgaaagc gatccggcac taaactgaat ctgcgccttc
     6961 gtacccagca ctttaccggc ttctggtgac cacggaacca cgccgccatt tacccgctgc
     7021 gcgctcagtt tccattcgct attcgggcta ttaaacgcca tatcacgcag ttgcagacga
     7081 tcggctttga acggtagcgg cgcggtctgg tcggtgagat tcagcgtccc gttttccagc
     7141 aggatggtat cgacatggcg tggttcggtc agttgccgac tgcttagcgc aatgtcgaca
     7201 ctttttgcca ccagggtcgc gggctgacca tcacgaccaa acgtgacgtt ctccagcacg
     7261 atatgagatg gcgcggaaaa acggtgatcc atcgccccga aggccagatg atagtcgcta
     7321 ttctcggaaa cccatgcgct gatatgttct gctccccagc gggtttgcag aagaaaatag
     7381 aggccagcga tcaccactaa cagagcgatg agaatgtaga gaagcagctt cccaataaat
     7441 ttcatggtct tccatcccgt gaaatgcaca taagggagtt atgcacgatt tacgcgcaat
     7501 cctcaaggcg ggaatggtga aagaggtgag actgcggcgg taattatctt accgccgcca
     7561 gtgaattact gtttttctgg tgggaaaatc agattcagca cgatggcagt aataccgccc
     7621 gcggcgatcc cggaggagag caggtttttc agccattcag gggcaaactg caaaatcagc
     7681 ggctgctgag acacgcccag accaaccgcc agcgacagcg cgataatcag aatcgcccga
     7741 cggttcagcg gctcacgaga aacgatacgc acaccggagg cggcgatggt gccaaacatt
     7801 acaagcgttg cgccgcccag aaccggttct ggaatgtgtt gtacaaaacc gctcactgcc
     7861 gggaacagac ccagcacgat caacatcagc gcgacgacaa aaccgacata gcggctggca
     7921 acaccagtca actggatcac tccgttgttt tgcccgaagc aggagttcgg gaaggtgtta
     7981 aacaccgccg aaacaaacga gttcaggccg tttgccagca cgccgccttt caggcgtttc
     8041 atgtacagcg gaccggacac tggctgttcg gaaacgtcag aggtcgccgt gatatcgcca
     8101 atggtttcca gcgaagtgat cataaagacc agcatcagcg gcagcagcag actccattca
     8161 atgccaagac cgtaatagag cggcgttggc accataatca gttcttgcgt catcggttcg
     8221 ttgctttctg gcaacatgcc cataaaccac gccagcgcat atccggccgc catcgcaatc
     8281 accagtgagg ccacgcgtaa gtaagggtta cgttgacggt taagcaggat aattaaggct
     8341 aagaccacgc ctgccagcag cagatttttc ggtgcgccga aggtgttatc gctcatggct
     8401 gcgtaaccgc cgccaatgga cgttaaccca acctgaatta gcgacaggcc gataatcatc
     8461 accacaacgc cagaaaccag cggcgtaata atgcggcgcg ccagatgcag aacgcgggag
     8521 agcaccatct cggtgcaact tgccagcatc aacgtgccga acaaagccgc catcatggta
     8581 ggaacatcag caccaccggt tttcagcgcg gtaccgccca taatcagcgg ggcaacaaag
     8641 ttgaagctgg tgccctgaat agacaacagc ccggagccaa ccggacccca ggccttaatt
     8701 tgaataatcg atgccacacc ggaggcaaac agcgacatac taataatgtg ttgcgtgtct
     8761 tgtgccggta aacccagcgc ctggcagatt aatagcgctg gcgtgatcac cgcaacgaac
     8821 atcgccagca gatgctgaca ggcggcaaac agggtttgag gaagcggcgg acgatcttca
     8881 agacggtaaa tcagttcgct gttttgagtc tgcgcaaccg gttgcgcatt ttctgactcg
     8941 agggtggaaa cagacatcga aaacaatccc gtggtagcaa agcgggcatt ttagctgacc
     9001 gaaagagaaa agcaaacgtt tgccacatcc tcatctaaat atttaaatga gataaatgca
     9061 cttttttata taacttttgg tatttttctg cttaaaatcc atgccatttg ttcattaaca
     9121 gctgagaatt tgacccagga aagcataact cgatacccgt catagttcat gaagttgcca
     9181 ctgcgcgatg aagtatgacg agtatgaaag agtgatgcgg atacaaagga gtaactatgt
     9241 ttcatctcga tactttagca acgcttgttg ccgcaacgct gacgttgctg ctcgggcgta
     9301 agttggtcca ttccgtctcc tttttgaaga aatacaccat accggaacct gttgcgggtg
     9361 gtttgttggt ggcgctggcg ctactagtac tgaaaaaaag catgggctgg gaagtcaact
     9421 ttgatatgtc cctgcgcgat ccgttaatgc tggctttctt cgccaccatt ggcctgaacg
     9481 ccaacattgc cagtttgcgt gccggtgggc gtgtggttgg catcttcttg attgtggttg
     9541 ttggtctgtt ggtgatgcaa aatgccattg gcattggtat ggctagcctg ttagggcttg
     9601 atccgctgat ggggctgttg gccggttcta ttacgctttc cggcggtcac ggtacgggcg
     9661 ctgcgtggag taaattgttc attgaacgtt atggcttcac caatgcgacg gaagtggcga
     9721 tggcctgtgc aacgttcggt ctggtgctgg gcggcttgat tggcggtccg gtggcgcgct
     9781 atctggtgaa acactccacc acgccgaacg gtattccgga tgaccaggaa gtcccgacgg
     9841 cgtttgaaaa gccggatgtg ggccgcatga tcacctcgct ggtgctgatt gaaactatcg
     9901 cgctgattgc tatctgcctg acggtgggga aaattgttgc gcaacttttg gctggcactg
     9961 cttttgaact gccgaccttc gtctgtgtcc tgtttgttgg cgtgattctg agcaacggtc
    10021 tgtcaatgat gggcttttac cgcgtctttg agcgtgcggt atccgtgctg ggtaacgtaa
    10081 gcctgtcgtt gttcctggcg atggcgctga tggggctgaa actgtgggag ctggcttcgc
    10141 tggcgctgcc gatgctggcg attctggtgg tacagaccat cttcatggcg ttgtatgcca
    10201 tcttcgttac ctggcgcatg atgggcaaaa actacgatgc ggcagtgctg gctgcgggtc
    10261 actgtggttt tggcctcggt gcaacgccaa cggcaatcgc caacatgcag gcgatcactg
    10321 aacgctttgg cccgtcgcac atggcgtttt tggtggtgcc gatggtcggt gcgttcttta
    10381 tcgatatcgt caatgcgctg gtgattaagc tgtatttaat gttgccgatt tttgccggtt
    10441 aaaaatgaaa aagcgtcgga aggaaccgga aacgctacgt gaacactgtc gccatatttt
    10501 tggtgatgag ccgccagtgc tctgtgtatg ggaaacggaa tttgattacg cagatgccga
    10561 actcaaggcg ctggcggcta aagagtggca gcagatcagc gaatgggatt tgagtgctta
    10621 ctatgtactc aatctcgttt ataacgaacc gatgcaaata gagttgtttc gctatctgtt
    10681 tccgctctgc ctggcgcaat ggcatgaaac cgttttagct ggcggttatg gtgatcactt
    10741 cgaagaatcc ttaatgaagg cattgtgtcg gccctatctc tggcaggaga tgatgaatgc
    10801 ttcccaacgc cagcaggtac ggcaattttt actggacacc gcgttgcagc gtatggataa
    10861 cgagcgtggt ttcaataacg ttctttgctg gctggcggtg ttcaatactc tgggtggggc
    10921 cgcgccgtta attcgttctc tctggtcgcg ctggtgggcg ctggatacgc caggaaaagc
    10981 ggtgtgtgct attcagtatg ccgcgcatct aatttatccc atagaagcca atccgttatg
    11041 gtcgcaagaa tggataggct gggggcatcc attaggccat aaagatggct ggtcctctga
    11101 taatcgcgct tttcttcgcc aaatgttaac acctgaaatg attgtggctg gcgtacaggc
    11161 agcggctgaa attctgcgtg gtgaaccgga aggcgcaatg gcggcgcgaa ttgcacagga
    11221 tgcgtatgag gcaatggata tcctcactat ccagattgaa gatttactgc gtgacttgtc
    11281 ttgtgatgag tctggtcatg cgttggagta acgttccgtc tccggcatcc agcgttctat
    11341 cagggctttt gcttgttgtg ggtaacgttc gtgaatatgt cgcgccaggc gctgaacttc
    11401 cgggatcatc gcctgatcgc gcagtaaatc cgccacttta aattcagcat tacccgtctg
    11461 acgcgtgcct aacaattcgc cagggccgcg aatctccaga tctttttgcg caatcacaaa
    11521 accgtcgtta ctgtcgcgca gcacttgcag gcgaatttgc gccgttttgg agagcggcgt
    11581 tttgtagagc agcacgcagt gagaagccac cgcgccacga cctacgcgcc cgcgcagctg
    11641 gtgtaactgc gctagaccca gacgctcagg gttttcgata atcatcagac tggcgttagg
    11701 cacatcaacg ccgacttcaa taacggtagt ggcaaccagc agttgtagct caccttgttt
    11761 aaacgacgcc atcaccgcct gtttctcggc tggtttcatc cgcccgtgta ccaggccaac
    11821 attcaattct ggtagcgcca gtttcaactc ttcccaggta gcttccgccg cctgcgcttc
    11881 cagtaactct gactcttcaa tcaacgtaca aacccagtac gcctgacgac cttcagtcat
    11941 gcaggcgtgg cgcacgcggt caatgatgtc ggtacggcgg gtatcaggaa tagcgaccgt
    12001 agtcactggc gtgcggcctg gcggcagctc atctatcacc gaggtatcga gatcggcata
    12061 cgccgtcatt gccagcgtgc gggggatcgg cgtggcggtc atgatcaact gatgcggatg
    12121 gaagccctgc tgctggcctt tctcccacaa tgccagacgc tgatgcacgc caaaacgatg
    12181 ctgttcgtcg ataatcacca gcgccagacc gttaaactgc acctgttcct ggaagatggc
    12241 gtgtgtaccg acaatcatct gcacctgacc gctggcgatg gcttcctgct gtgccagtcg
    12301 cgctttacct ttctgcttac cggcgagcca gcccacttcg ataccgagcg gtgcaaacca
    12361 gttgcggaag ttattggcgt gctgctcggc gagtaattcg gttggtgcca tcagggccac
    12421 ctgtttgccg tgggcaatcg cacgcagcgc ggcgagggcg gcgaccagtg ttttaccgga
    12481 acctacatcg ccctgcacca ggcgcatcat tggaacatcc agcgccatat catgctcgat
    12541 ctccgccact acgcgtgcct gtgcgcccgt tggcttgaac ggtaaggcgg cgaggagttt
    12601 atttttcagc gcgtcattgg cgctcagcgg ctgggcatga aaacgctgtg ctccggcacg
    12661 taaggctaac atgctgaggt tgtgcgccag cagttcttcc agaatcagac gacgttgcgc
    12721 cggatgctgc ccggtttcca gatcgctaag ctgtagcgtc ggcggcgggc ggtgcaaagt
    12781 gcgcaacgct tccggtagcg tcatcattcc ttgtgacagt tccggcggca ggagttcttc
    12841 aatggcgcag gtgtcgagca gatccagcgc ctggtcggtt aatttacgca gcgtggcctg
    12901 ctttacgcct tccgttgttg gataaaccgg cgtgagcgtt tcctgtaatt ctggcgtgct
    12961 gagatcgccc tgcacgcggt attccgggtg gatcatctcc gcaccatatt taccgcgctt
    13021 tgcttcgcca taagccagta cacggcggcc cgtcgccagg ctatttttca ttgccgcgct
    13081 gaaattgaaa aagcgcatgg tgaggatgcc ggaaccgtcg ctgatctggc aggtcatcat
    13141 ccgccgaccg ccgaaggaga tattgcagtt cagcacttcg ccttccaccg tggcataaac
    13201 gcccggcagt agttctccga tggggtagag atgggtgcga tcttcgtagc gcaggggaag
    13261 gtgtaagagt agatcctgca cggtatgcag gttgattttc gccagtttgt tactaagtgc
    13321 tgcgccaacg cccgttaggg aactgagtgg gacagcatct aacaggcgac ctttcatggc
    13381 acttaccctg cagcctgcat agtagcccac cagtcggcat cagcttcgat ctcgccttgc
    13441 tgattgacgt ggggataagg caggcctttg cgttttgcga ctttcgccag caccggatag
    13501 ccgccttcaa acaacaggcg ttgttgctct gcttccggca acatgctgtt ttcacgcagg
    13561 tacatgcctg cattttgccg ctgacgctgg gcttcgtaaa gaatgagggc tgaggcaacg
    13621 gaaacattca gcgactgcac catgccgatc atcggaatga tgatgtcctg atccgccagg
    13681 gccaatgctt cctgcgtgat gcccgttttc tcctgtccca tcaaaatgca ggtcgggcga
    13741 gtgtaatcaa tttcgcggaa atcgacagcg ttatcagaaa gatgggttgc cagaatctgc
    13801 atgccctggc ctttgagatg agcgacggca tcgccaatgg tgcggtgtgt tttcacctgt
    13861 acccagctgt tactacccgc cgctgccgaa gccatggtgc gcatgcggct accaggccag
    13921 acggcgtgaa cttcatgtac gccaacggca tctgcggtac gaataatcgc agaaacgtta
    13981 tgaggtttgt ggacctgctc catgcagacg gtcagatcag gctgccgcct ggcgagcatt
    14041 tcgcagatgc gtgcataacg tgttgggttc ataaaacatt aatttcggtt tcgggtgact
    14101 ttaatcacgt ctggcatcac gcggattttg cgcatgatat tcgccagatg cacacggtca
    14161 cgggcggtca gacgaataaa ggcgctgtag acgcgaccat ctttctcttc cgtattcaaa
    14221 ctttgaatat tcgaagtcgt ggtgttaatt gccgccgtca ggtttgccag cgcgccctga
    14281 tgattgaaca tctccacctt gatttcggtg atgaactcct gcgccgtctc tttatcccat
    14341 tccacagcca taaacttctc tggctctttc tggtagccac ggatattacg gcaggattca
    14401 tggtggatca ccagaccttt accggggctg acgtgggcga taatcgggtc gccaggaata
    14461 gggcggcagc atttcgcaaa ggtgatcagc acgccatcgg cacctttaat gggcagatgt
    14521 ccgtggcttt gggttgccgg tggaatggag gcgtccccat gttgcagatt tttcgcgacc
    14581 accacgctca ttgcgttacc aagtccgatt tctgccagca gatcgtcaag cgttgccagc
    14641 ttcatgcgat ccagctcgcg ctgaatattt tcctgcggga tttcattcag cttacggcta
    14701 ccacccaaag catggttgag cagacgacgg cccaggctta cagaatcatc acgcttgagg
    14761 tttttcagca actgacgaat tttggcgcgc gcttttgagc taacgacaaa gttcagccac
    14821 gcggcattcg ggcgagcgcc cggtgcggta atgatttcaa cggtttgacc gctggtaagc
    14881 ggctgcgaca gcgggtaagg ctggcggtca acgcgtgcgc ccacgcaggc atgaccgata
    14941 tcggtatgca ctgcataagc gaagtcgacg ggcgttgcac cggcaggcag ctcgacaatt
    15001 cgcccttccg gtgtgaaaac gtaaatctca tccgggaaga gatcggattt aacgctctcg
    15061 ataaattcaa acgaactacc ggcgctctgt tgcagctcca gcaggctttg catccagcgc
    15121 tgggcgcgga tttgcgcggt ggtgctggtt tcgccgtgct ctttataagc ccagtgcgcg
    15181 gcaacaccca tctccgccat ctgatccata tcttcggtac ggatctggac ctcaaccggc
    15241 acaccgtgcg ggccgatcat cgaggtgtgc aaagactgat agccgttcgc ttttggaatg
    15301 gcgatgtagt ctttcacgcg acccggacgc ggcttgtaca ggctatgcat ttgcccgagc
    15361 acgcgataac aggtgtcaga atcattgacg atcacgcgga aagcgtagat gtccatgatt
    15421 gagtgaaaac gctgctcttt gagcaccatt ttgcagtaaa tcgaataaag atgcttttcg
    15481 cgaccactga cgcggcacgg tattcccgct tcctgcaaac gcccttcgat ttcagagagg
    15541 attttttgga tcatctcttt acggttgccg cgcgcggctt tcaccacttc tttaattacg
    15601 cggtaacgat tgggatacag cgcctcaaaa cccagctctt cgagttcggt tttaatgtgg
    15661 tggataccta aacggtgcgc cagcgggcta taaatttcga gagtttcacg ggcgatgcgg
    15721 cgacgtttgt ccgggcgaag tgagcccagc gtgcgcatgt tgtgggtacg gtcggcaagt
    15781 ttgatgagga tgacgcggat atcctgcacc atcgccataa tcatcttgcg aaagttttcg
    15841 gcctgcgcct ctttcttatc gcggaacttg agtttatcaa gtttcgacac cccctctacc
    15901 agctcggcga cgcttttacc aaaaagctgt tccatatcct ggtaggtggc gggagtatct
    15961 tcaatcacgt catgcagcag cgccgccatc agcgtttcat agtcgagttt catctcggcc
    16021 agaatgcagg caaccgctac cgggtgcgtg atatagggtt caccgcttga acgtgtttgc
    16081 ccctcgtgag catcacgtgc aacgagatac gcctgccgca gacgcttgat ttggtcttcc
    16141 ggcaggtagg tttgaatcag ttgattcagg ctttcaaaca gatacaaggg cgacccgctt
    16201 tgtgattaac gacgaccttc agcaatagcg gtaacggctt gtaattcagc ggcttcctgc
    16261 tcttgctgtt cctggcgttc gcgaacgtcg aggatctggt tgttgatcag accttcttcg
    16321 atttcgcgca gcgcgattac agtggtttta tcgttttctt ccggtaccag cggatccttt
    16381 ccgcctacct gcatctgacg agcgcgacgc gcggcgacca gtaccaggtc aaaacggtta
    16441 ccaattttct ctacagcgtc ctgaacagtt acgcgtgcca tacttaaaaa gctccacagg
    16501 tgaagaaatg actgggcatg atactgaaat caggttcagt ctgccaacaa tttgctgatt
    16561 aaagcgtcat gacgctgctt ttggcggctc atgcgcagac gttcggcgcg aataatggtc
    16621 ttcaaatcgg tcaacgcggt atcgaagtca tcattcacaa tcagataatc atattcggcg
    16681 taatggctca tttctgcaac agcttgcgcc atacgctttg caatgacctc ttcgctgtcc
    16741 tgaccgcgac cgcgtagacg gcggtccagt tcaattttgg acggcggtaa aataaagata
    16801 ctccgcgcgt gcggcatctt ctggcgaatt tgctgcgcac cctgccagtc gatatcgaga
    16861 aaaacatcga caccggtcgc cagtacttgc tcaatggcct cacgcgaagt gccatagtaa
    16921 ttaccaaaaa cttctgcgtg ttcgaggaac gcatctctgc taatcatttc tttaaattca
    16981 tcatgattaa caaagaaata atgttcaccg tggacttcac caggacgcgg ttggcgtgtg
    17041 gtgtgtgaaa cagaaacctg ggtgtcatac aacggttggg tttttaataa agcctgaatc
    17101 aggctggatt tacccgcgcc actgggggca gaaacaatat aaagcgtgcc ttgagccatg
    17161 agtatctttc gtatgtgatt agcgaaataa agcctacata cgagcttatt atacacggcg
    17221 cggccatgtg acgtagccct tgctcacact ttttgcgcaa agttgttgcg ttttgcgtag
    17281 ctgtttccgc atttgcctgt gatttactgc aataaagacg attctcagga agcgtcctcg
    17341 cagactccgt ttttgccact cggctttgcc ttcaccacgt tgtatatctg ccagcctgac
    17401 tacagcggag gtgaagatga aagtatggat ggcgatatta ataagtatct tgtgctggca
    17461 atcatctgtg tgggcggtct gtccggcctg gtcgccagcc agagcacagg aagaaatttc
    17521 ccgcctgcaa cagcaaataa aacagtggga cgatgactac tggaaggaag gaaaaagtga
    17581 ggtggaagac ggtgtttacg atcagttaag cgcccgtctt acgcagtggc aacgctgctt
    17641 tgggagcgag ccccgcgacg tgatgatgcc gcctttaaat ggtgcggtta tgcatccggt
    17701 tgcccatacg ggtgtacgca aaatggtgga taaaaacgca ttaagtctgt ggatgcgaga
    17761 acgtagcgat ctttgggtgc agccaaaagt tgatggcgtt gcggtaaccc tggtttatcg
    17821 ggacgggaaa ctgaacaaag caatcagtcg cggtaatggc ctgaaaggcg aggactggac
    17881 gcagaaagtt agcttaattt ccgctgtgcc gcaaaccgtt agcgggcctt tagccaacag
    17941 tacgcttcag ggggaaatat ttctccagcg cgaggggcat atccaacaac aaatgggggg
    18001 aataaatgcc cgcgcaaaag ttgctggctt gatgatgcgc caggacgata gcgacacgct
    18061 gaattctctg ggcgtttttg tctgggcatg gccggatgga ccgcagttaa tgtctgatcg
    18121 tttaaaagag ctggctaccg caggttttac tctaacgcag acgtataccc gtgcggtgaa
    18181 aaatgctgat gaagttgcgc gcgtacgcaa tgagtggtgg aaagcggaat tacccttcgt
    18241 caccgatggc gtagttgtgc gaggggcgaa agaacccgaa tcccgccatt ggctaccagg
    18301 ccaggcagaa tggctggtgg cctggaaata tcaacctgta gctcaggttg ccgaagtgaa
    18361 ggcgattcag tttgcggtgg gtaagagcgg taaaatatcg gtggttgcgt cactcgcacc
    18421 tgtcatgctg gatgataaaa aagtccagcg ggtgaatatt ggttccgtca ggcgctggca
    18481 ggagtgggat attgcgcctg gtgatcagat cctcgtcagc cttgccggtc agggtattcc
    18541 tcgcattgat gatgtggtct ggcgcggtgc agaacgtaca aaaccgacac caccagaaaa
    18601 ccgctttaac tcgttgacct gctactttgc ttctgatgtt tgtcaggaac agttcatttc
    18661 acgcttagtc tggctggggt caaaacaggt tctggggctg gatggcattg gtgaggccgg
    18721 ctggcgcgcg ctgcatcaga ctcatcgctt tgagcatatc ttttcctggc ttttattaac
    18781 gccagagcaa ttacagaaca cgccggggat cgcgaaaagt aaaagtgcgc agctatggca
    18841 tcagtttaat ctggctcgta agcagccttt tactcgctgg gtgatggcaa tgggaatacc
    18901 gctaacccgg gcggcgctta atgccagtga tgaacggtcc tggtcgcaac ttttatttag
    18961 cacggagcag ttctggcagc agctgccggg gactggatcg gggcgcgcca gacaggttat
    19021 tgaatggaag gaaaatgcgc aaatcaagaa gctgggcaat tggctggctg cccagcagat
    19081 cacaggtttt gaaccttagt gtccttcgtg gctgtagtaa aagaccggta atcccagctt
    19141 aaggcgtagc gccagtaagc gggcaaaaaa gccgaatacc agggtggaga taattaccac
    19201 atcatggtta gaaacatagt gttgcagcgc gatgtacagc acggcggagg caaatgagac
    19261 accggcatac aactctttct ggaataccag tgggatacgt ttacagaaca tatcgcgcaa
    19321 aacgccgcca aacacgccgg tggttaccgc cgcgacaacg gcaataattg ggccgtgccc
    19381 catatccagt gcaacctgtg cgccgatgat agaaaagacc accagtccaa gcgcatcgag
    19441 caccagaaac actttgcgca ggtaaggcat tacaggagca acgatagtgg tgagtactgc
    19501 ggcggtagcg acgataatca catattccgg gtgtttgacc cagccgagcg gatagtggcc
    19561 cagcagaata tcgcgcactg accctccgcc aattgcggtg gcggtcgcaa taataattac
    19621 gccaaatgta tccatgcggc gtcgcccggc cgccagagcc cccgtcatgg cttcggcagt
    19681 aatgccaacc aaatacaaaa tgtgtaacag catggttctc tccctaaaaa tctgtctggc
    19741 agggtagcga tttgtgcgta ttgtcacgat tgagattttt taaggtgaga ggaaattgat
    19801 aacttgtagt tatgtgaaat cgctattttc tgtagcgtag atgcattctt ctgacttctt
    19861 gtctgagaaa atgcataaga aaaaataggt taaaactgag gggggaaaag aaaggtgcaa
    19921 tgtttagcca ttgtgttcac tgcacctgga ccttggctta cgcatcgggt gtctcaatca
    19981 acaaaaagag tttttcgttt ctgctatttc ttctctgtaa tttttacact gttttccaac
    20041 tgcttgatgc tgacctgcgg ggtcggcaac tcctcaggca tggttccgcc aagttcctga
    20101 atggtttgcc tcaccttgct acccacgtca aagtgtgtgg tattagcttg ctgtgctgcc
    20161 tcaaccaatt gtttgttatg ttctttcaac tcatttctca gaaacagacg cttttcgtct
    20221 tcacgaagtt gcttaaatgc ttcatcgtca gcaagctcct gccgtcgggt ctggatagca
    20281 aaataagtct gccctgccgc aatgaccggt ttcgcggggt cgccgttttg tactaccagg
    20341 taacaggcat agcgggagag tacgatgtct tttaatgctc ttttggcatt tgagccaatt
    20401 ttgaccatat cgaggatctc ctcgaaatgg tcatcaattg tgtgaccact gttctcacag
    20461 gcctctttgg cgcgcgttaa tacaggtata aagtgacgat actctgaata acccagtagt
    20521 ttcccaagtt gtcttgctga ccattgttct gctccgtttg cattaatcag ttttatctct
    20581 tcaaaaggtt gatgatgttc gttcattgcc cactccatgt gatatcgatt gcaaagaaag
    20641 taaatctgta actgtattta tatacagttg tgagtatctc acgttctcag tgtgctgggc
    20701 aaaaaaacat cgcaaaatct ggagcaggca cgcaaaacag aattgaagtt gcggggaaga
    20761 aaaatgccgc tctgtttgag tgggcggcat ttgtgaaggc tgaaaagtaa gaattagtgt
    20821 tacgagttac tcaatattct gaatctgctc gcgcatctgc tcaatcaaca ctttcagctc
    20881 gatggcggag tttgtcactt cggcattgat agatttcgac gcaagagtgt tcgactcgcg
    20941 gttgaattcc tgcatcataa agtccagacg acgaccaacg gcttcttttt tcttcagaat
    21001 gttgtaggtc tctttgacat gcgcttccag gcggtccagt tcttcggcaa cgtcaattcg
    21061 ttgtgccagc agaaccaact cctgctccag acggttgttt tccaactgaa cctgagcatc
    21121 ttccagcttc gcgaccagac gctcacgctg ccattgcagg atttccggca tatgggcgcg
    21181 gactttgacc acttcggcgg tgacgccttc cagacgctgc tcgatcaatg ctttcagcgc
    21241 ctgaccttcg gtttcgcgcg cgacgataaa gtcgtccagg gtaccatcca acgccgcgag
    21301 aatttcagcg gcaatcgcgt cgagatcctg ctcctgggct gccatcacgc ccggccagcg
    21361 tagaatatca accgggttga tttccccttc gtcactctgc atttttaccc agttcgcggc
    21421 agttaccagc tgtttagcca gtttttcgtt gaggatcagc tcaccttgtg cgctaacatc
    21481 tggctcatag cgcagggtac attccacttt accgcgcgtc aggcgagaac gaatacgctc
    21541 gcgaacgaca ggttcaaggc tacggaactg ctccggcaga cgaaagtaag tttccagata
    21601 acgctggttt accgagcgca tttcccaggt tgcgctcccc cattcaccct tgatttcacg
    21661 ccgggcgtag gcggtcatac tgcggatcat agacgttcct gtttataaaa ggagaggtgg
    21721 gaggattata gccatcgatg ccttgtcagg ataggaataa ccgccggaag tccgtataat
    21781 gcgcagccac atttgtttca agccggagat ttcaatatgc gtccagcagg ccgtagcaat
    21841 aatcaggtgc gtcccgttac cctgactcgt aactatacaa aacatgcaga aggctcggtg
    21901 ctggtcgaat ttggcgatac caaagtgttg tgtaccgcct ctattgaaga aggcgtgccg
    21961 cgcttcctga aaggtcaggg ccagggctgg atcaccgcag agtacggtat gctgccgcgt
    22021 tctacccaca cccgtaacgc tcgtgaagca gcgaaaggta agcagggtgg acgcacaatg
    22081 gaaatccagc gtctgatcgc ccgtgcactt cgcgcggcag tagatttgaa agcgctgggt
    22141 gagttcacca ttacgctgga ctgcgacgtg cttcaggctg atggtggcac gcgtaccgct
    22201 tcgattacgg gtgcctgtgt ggcgctggca gatgcgctac agaagctggt ggaaaacggc
    22261 aagctgaaaa ccaatccaat gaaagggatg gtagccgcgg tttctgtcgg tattgtaaac
    22321 ggtgaagcaa tttgcgatct ggaatacgtt gaagactcag ccgcagagac cgacatgaac
    22381 gtagtgatga ccgaagacgg gcgcattatt gaagtgcagg ggacggcaga aggcgagccg
    22441 ttcacccatg aagagttact caccttgttg gcgctggccc gagggggaat cgaatccatt
    22501 gtagcgacgc agaaggcggc gctggcaaac tgatttttaa ggcgactgat gagtcgcctt
    22561 ttttttgtct gtagaaaagt aagatgagga gcgaaggcat gaaaccatat cagcgccagt
    22621 ttattgaatt tgcgcttagc aagcaggtgt taaagtttgg cgagtttacg ttgaagtccg
    22681 ggcgcaaaag cccctatttc ttcaacgccg ggctgtttaa taccgggcgc gatctggcac
    22741 tgttaggccg tttttacgct gaagcgttgg tggattccgg cattgagttc gatctgctgt
    22801 ttggccctgc ttacaaaggg atcccgattg ccaccacaac cgccgtggcg ctggcggagc
    22861 atcatgacct ggacctgccg tactgcttta accgcaaaga agcaaaagat cacggtgaag
    22921 gcggcaatct ggttggtagc gcgttacaag gacgcgtaat gctggtagat gatgtgatca
    22981 ccgccggaac ggcgattcgt gagtcgatgg aaattattca ggccaatggc gcgacgcttg
    23041 ctggcgtgtt gatttcgctc gatcgtcagg aacgggggcg cggcgagatt tcggcgattc
    23101 aggaagttga gcgtgattac aactgcaaag tgatctctat catcaccctg aaagacctga
    23161 ttgcttacct ggaagagaag ccggaaatgg cggaacatct ggcggcggtt aaggcctatc
    23221 gcgaagagtt cggcgtttaa aaatatgcgc tgtgcagggt ttttgctgtg cgcagcgtga
    23281 tgcgcttcaa gatatcgtgt taatctgctt tcgccagcag tggcaatagc gtttccggcc
    23341 ttttgtgccg ggagggtcgg cgagtcgctg acttaacgcc agtagtatgt ccatataccc
    23401 aaagtcgctt cattgtacct gagtacgctt cgcgtacgtc gcgctgacgc gctcagtaca
    23461 gttacgcgcc ttcgggatgg tttaatggta ttgccgctgt tggcgcctct tttggccgcc
    23521 gtgatgtgga gagtggaatg gatgctaccc ggacaaccct tctggcgctc gatttgttcg
    23581 gctcgccggg ctggagcgcc gataaagaaa tacagcgact gcatgcgctc agtaatcatg
    23641 ccggacgcca ttaccgacgc attattcttt ctaaacgcca cggtggtcag cggctggtgt
    23701 tagcccctga ttacttgctc aaaaccgtac agcgcaacat tcttaagaac gtcctttcac
    23761 aatttccgct ttcccctttt gctacagcct accgaccagg ttgcccaatc gtcagcaacg
    23821 cgcagccaca ctgccaacag ccgcagatcc tgaaactcga tatcgaaaac tttttcgata
    23881 gcattagctg gttacaggtc tggcgtgtgt ttcgccaggc ccagttgcca cgtaatgtgg
    23941 taaccatgct gacctggatt tgttgttata acgacgcgtt accgcagggg gcaccaactt
    24001 cgccagccat ttccaatctt gtgatgcgcc gttttgatga acgcataggg gaatggtgtc
    24061 aggctcgggg aattacctac acccgctact gcgatgacat gaccttttca ggtcacttca
    24121 atgcccgcca ggttaaaaat aaagtgtgcg gattgttagc ggagctgggc ctgagcctca
    24181 ataaacgcaa aggctgcctg atagctgcct gtaagcgcca gcaagtaacc gggattgttg
    24241 ttaatcacaa gccacagctt gcccgtgaag cgcgccgggc gctgcgtcag gaggtgcatt
    24301 tgtgccaaaa atatggcgtt atttcgcatc ttagtcatcg tggtgaactt gatccttctg
    24361 gcgatctcca cgcacaggca acggcgtatc tttatgcttt gcagggaaga ataaactggt
    24421 tattgcaaat caaccctgag gatgaggcct ttcaacaggc gagagagagt gtaaagcgaa
    24481 tgctggttgc atggtaagaa aagcgtcagg cagacgtttc tgcctgaccg tttaggggag
    24541 aattactgca actgcgcggc aattagcggc cagcgggcgt caaaatcatc cgtcgggcgg
    24601 tatttaaact cgctgcggac aaaacgtgac agcatacctt cacagaaggc caggatctgg
    24661 cttgccagca gggtttcatc ggtggtgtaa ccttcaccct cacgcattct cttttcacgc
    24721 aatacctgac gcagctgcgc ttcaatacgc tcgaacagct ggttgatgcg cccttgcagg
    24781 cgatcctgtt caaacattag cgcatgcccg gtaaggatgc gggtcaggcc aggattacgc
    24841 tcaccaaaac cgagaagcag caacacaatc agacgcaggc gcgctgtggt gtctttctca
    24901 tctttcagaa tcaggttgat gcgagtaatc aggctatctt cgataaactc aatcaggcta
    24961 tcgaacatgc gggtcttact ggggaagtgg cgatatagtg ccgcttcgga aacgccgaca
    25021 gaggcagcca gttttgccgt cgtgatacgt tggcttccat cgctggattc cagcatcagc
    25081 gccagagact gaagtatttc ctcgcgacgg ttccttttcg cagtttgttt ttctgccatg
    25141 ttacaaaata cccctgaaaa taagcacttg ccaggcggca cccacgctat gaccgcaaac
    25201 gaaatgtttg cggctatgtt atgacgttat tcggatgcgt atgtgttact gacgaccaga
    25261 gtgaccaaag ccgccttcac cgcggtcggt ggcgtcgaaa tcttccacca gattaaattc
    25321 agcctgtact accggaacaa aaatcatctg ggcgatgcgt tcgccaggtt gaatggtgaa
    25381 gctgtcctga ccacggttcc acacggaaat catcaactgg ccctgatagt cggaatcgat
    25441 caatcctacc aggttaccaa gcacgatacc gtgcttatgt cccaatccgg agcgcggcag
    25501 catcattgcc gccagtgaag gatcggcaat atgaatcgcc agcccggtcg gaaccagcgt
    25561 agtgtcaccc ggagccagtt ctacggcgtc gtcgagacag gcacgcaggt caagtccggc
    25621 agagccagag gtggcataag tcgggagcgg aaattccttc ccaacgcgcg ggtccagaat
    25681 cttaacgtcg atttttttca tcataacggg tcacgatctc gtcgagtaat aattggccaa
    25741 ggagctcttt gcgctcaagc ggtaagactt tatctccgtc ctgccagaaa aggtgtaatg
    25801 cgttgttgtc gctgttaaat ccttgagttg gctgggaaac atcgttcgcg cagatcagat
    25861 caaggttttt acggatacgt ttttgccggg cgtattcttc cacattattt gtttcggcgg
    25921 caaatccaac gacgtagggt cgatggtctt ttagtgcggc aacgcctgcg acgatatcgg
    25981 ggtttttaac catttttatt gtgaattcat caccctgcgt ggcctgcttt ttgatttttt
    26041 ctggggccac ggtagctgcg cgataatccg ccacggcggc gcaaccgata aaaatatttt
    26101 gctgctgtac agaagcattc acggcggctt ccatttccag cgcggtcatc acatcaacac
    26161 gtttaacaaa cggtggtgtc ggtagtgaaa ctggacctga taccagcgtg acgttcgcgc
    26221 cacgacgggc agcggcggca gcgatagcaa aacccatctt gccggagctg tgattagaga
    26281 tataacgcac cggatcgagc ggttcacgcg tcggaccggc ggtaatcata atgttcagat
    26341 gtttcaggtc gttgacgggc gaaaaatgcg ctaccgccat atccacaatg gttaacggat
    26401 cgagcattcg cccagggccg atatcaccac aagcctgact gccactgtct ggcccccaga
    26461 tgagcaaacc acgggaggca agcacctcta aattatgctg cgtggcagcg gcacggtaca
    26521 tctgctggtt catggcgggg agcacggcta cacgcgcagg tgtagccaga caaatcgtcg
    26581 ataccagatc atttgccatt ccggcagcaa cacgggcaat caaatctgcc gtggcagggg
    26641 cgagaatcac taaatcagcc catttaccca gctcaatatg gcccatagcg gcttcggctg
    26701 ccgggtccag cagactgtcg gaaacgggat aaccagaaac cgcctgcaaa ctaagtgggg
    26761 tgataaaggc ttttgccgct tcggtcatgg ctacgcggac gtcggccccg cgatcgcgca
    26821 aacgacgcac cagttcaggg gttttatagg cagcaatacc gccgctaacg ccgagaacga
    26881 tttttttacc ggccaggctc atgatgattt ttcctgttgg gtgacaccag aagttggcga
    26941 ttttatcaca atacttttgt tgtcgtgcct tcacctgaga ttcactttgc gaggcgcttt
    27001 ccaggattga aaactggccg tcgatttaac ggaacggcta tgacaggatg cgagcaccac
    27061 aaaggaggtg aaggtgaaaa acaatgcaca gctgttgatg ccgcgcgaaa aaatgctgaa
    27121 gtttggtatt agcgccttaa cggatgtcga gctgctggcg ctatttctgc gtaccggaac
    27181 gcgcggtaaa gatgtattaa ccctggcaaa agagatgttg gagaatttcg gctctcttta
    27241 tggcttgtta acctctgaat atgaacaatt tagtggtgtt catggaattg gcgtagcgaa
    27301 atttgcccag ttaaagggga ttgctgaact ggcgcggcgt tactacaacg tgcggatgcg
    27361 tgaagaaagc cctttactca gcccggagat gacgcgggaa tttttacaaa gccagctcac
    27421 gggtgaggag cgggagatct ttatggtgat ctttctcgac tcacaacacc gggttataac
    27481 gcatagccga cttttttccg gcacgctaaa ccatgttgaa gtccatcctc gggaaattat
    27541 ccgcgaagcg ataaaaataa acgcctcggc gctgatcctt gcacataatc acccttcggg
    27601 ttgtgctgaa cccagtaaag cggataaact cattactgaa cggataataa agagttgtca
    27661 gttcatggat ttacgcgtgc tcgaccatat cgtgattggg cgtggagagt atgtttcttt
    27721 tgccgaacgc ggctggattt aacccgctat gcgcgatcct tcgggatctt tgtctgttcg
    27781 ggacttgagc acatcgctga gtcagcgtat actacgccac ctttgagaat ctcgggtttg
    27841 gcatttgggc ctggcaatcg agagttcaca tagaactgcg atgaccgggc tgtaaagcct
    27901 gacgaggcgc caatacccca tacgaagctc gagctaattt gatttttgga gaatagacat
    27961 gtcccgagtc tgccaagtta ctggcaagcg tccggtgacc ggtaacaacc gttcccacgc
    28021 actgaacgcg actaaacgcc gtttcctgcc gaacctgcac tctcaccgtt tctgggttga
    28081 gagcgagaag cgttttgtca ccctgcgcgt atctgctaaa ggtatgcgtg taatcgataa
    28141 aaaaggcatc gatacagttc tggctgaact gcgtgcccgt ggcgaaaagt actaagtact
    28201 tagaggaaat aaatcatggc taaaggtatt cgtgagaaaa tcaagctggt ttcttctgct
    28261 ggtactggtc acttctatac cactacgaag aacaaacgta ctaagccgga aaaactggaa
    28321 ctgaaaaaat tcgatccagt tgttcgtcag cacgtgatct acaaagaagc gaaaatcaaa
    28381 taattctcgc tttgatgtaa caaaaaacct cgctccggcg gggttttttg ttatctgctt
    28441 gcccccatat tgattgcatc tgttcattcc tggagatgct atgcctgaat tacccgaagt
    28501 tgaaaccagc cgtcgcggca tagaaccgca tctcgttggt gcaaccattc ttcatgcagt
    28561 ggtgcgcaac ggacgcttgc gctggccggt ttcagaagag atctaccgtt taagcgacca
    28621 accagtgctt agcgtgcagc ggcgggctaa atatttgctg ctggagctgc ctgagggctg
    28681 gattatcatt catttgggga tgtctggcag cctgcgcatc cttccagaag aacttccccc
    28741 tgaaaagcat gaccatgtgg atttggtgat gagcaacggt aaagtgctgc gctacaccga
    28801 tccgcgccgc tttggtgcct ggctgtggac caaagagctg gaagggcata atgtgctggc
    28861 ccatcttgga ccggagccgc tcagcgacga tttcaatggt gagtatctgc atcagaagtg
    28921 cgcgaagaaa aaaacggcga ttaaaccgtg gctgatggat aacaagctgg tggtaggggt
    28981 agggaatatc tatgccagcg aatcactgtt tgcggcgggg atccatccgg atcggctggc
    29041 gtcatcactg tcgctggcag agtgtgaatt gttagctcgg gtgattaaag cggtgttgct
    29101 gcgttcgatt gagcagggtg gtacaacgct gaaagatttt ctgcaaagtg atggtaaacc
    29161 gggctatttc gcgcaggaat tgcaggttta cgggcgaaaa ggcgagccgt gtcgggtgtg
    29221 cggtacgccg attgtggcga ctaaacatgc gcagcgggca acgttttatt gtcggcagtg
    29281 ccagaagtaa ttcatgcgcg ccggatggca taccatccgg cataaacgct acgctaactt
    29341 cgccatcagc gcctgatgga cattctccgg caggaaatgg gtgacatcgc cctgatggcg
    29401 cgccacctct ttcaccaacg atgaagagat aaacgaccac tctttcgacg gcatcagaaa
    29461 cacactttcc agttccggca ttaagtggcg attcatatgc gccagctgca tttcatattc
    29521 aaaatccgcc accgcacgca ggccacgaat cagcaccgta gcgtgttgat tacgggcgaa
    29581 gttcgccatc aaatcactaa acccgaccac ttccacgttc cccagatgcg cggttgcctg
    29641 ctgtgccagc gccacacgct cttccagggt aaacatcggt tttttactgg ggctggcggc
    29701 aatcgccaga ataacgtgat cgaacatttg cgtggcgcgc gtcacgatat cgatatgacc
    29761 attggtaatg ggatcgaaag taccaggata aatcgcccgt ttttgcataa caacctcaat
    29821 gcgttttcgg tggcaggtaa ggttccagca gttgaagcag acgctgtagc gcgccctggt
    29881 tttgatacag tacttcaacg gcatgacggc catagaaact acggtaatcg gcgtcggtga
    29941 gtaaagagga cacctctttt gcaagcgtag tggcatcggt aacggtaatc agcccgcttg
    30001 cctgctccag ccgcgcgcaa atgtctttaa agttaaaagt atgcggcccc atcaataccg
    30061 gaatagcgtg tgcggcagct tccagcggat tatgcccacc acgttcaacc agtgaaccgc
    30121 caacaaaggc gagatcggca atgccgtaca gtaacatcaa ctcgcccatc gtatcgccaa
    30181 ccacaacctg cgtgctggtg gaggggactt cccctgaaga gcgtgtgata tagcttagtc
    30241 cagcctggcg gacaagatta atcgcatccg ggaagcgttc tggatgacgg ggtaccagga
    30301 tgagcaataa attcgggaat tgctgtaaca atgcctgatg tgcggcgatc accacactct
    30361 cttcgccttc gtgagtgctg gtggcaatcc ataccgggcg gtgtggtgcc cactggcggc
    30421 gcagcgtcac ggctttagca gccaactgcg gcgttacaga aatatcgaat ttcaggctac
    30481 cggtaacggt cacctgatta ttttttgcgc ccagcgccac aaaacgtgca ccatcttctt
    30541 cattttgcgc agcaatcagc gtaatacgac gcagcaagcg acggacgaat ttacccagtt
    30601 tggcataacc tgcggccgag cgggcagaga gtcgcgcgtt agcgatcacc agcggaattt
    30661 tacgtttgtg tagcgccgca atcaggttag gccatagttc ggtttccata atcaacacca
    30721 gtttggggtc gactttattc aggaaacggt tgagcgcatc gggcaggtca tacggcagat
    30781 aaacgtgctg aacatccttc ccgaaagccg attgtacgcg ctccgaaccg gttggcgtca
    30841 tggttgtaac ggtaatcggt aaatcaggat aacgatggcg cagcgcgcgc accaacggga
    30901 tcgccgccag agtttcaccg acggagacgg agtgcagcat aatgccgcct ggttttagcg
    30961 gatggcggta aaaaccgtaa cgttcacccc agcgttttcg ataggccgga gccttacgtc
    31021 cgcgcaccca gagccgtatc cagatcagcg gctgaataag gtagagaagg gcggtgtaaa
    31081 gcaattcgag catagtaaat agctgactta tggatgtgct ggggattcta tgtatttagc
    31141 tgtggcttta ccattacttt tcccgttttt gacttaaata gcaatttacc cgccaattct
    31201 tgctgaacat ctatactttt tttgtatttt tatgcgattc attgatttct gctgtacgtg
    31261 caggaaggaa tcgcagagca acagtgaaag aatatggttg ctaatgattt tatttgattg
    31321 atttctttat tgaaaatcaa caatgtgctt aaaaatcatt cgggtcggat tgccgcgaaa
    31381 gaagtgatac actagcgcgt caatatcagt gcggtatcag tattcaggta gctgttgagc
    31441 ctggggcggt agcgtgcttt ttctgcttca ccttaatcgg ataatctcaa caaaaagagt
    31501 tacttgtgga taagccattt cgaagaattt tgctcattaa gatgcgtttt catggggata
    31561 tgttattaac tactcccgtc attagttcgc tgaaaaaaaa ttaccctgac gcaaaaatcg
    31621 atgtgctgct ttatcaggac accatcccga tcctgtctga aaatccagag attaacgcgc
    31681 tctacggcat aaaaaataaa aaagcaaaag cctcagaaaa aattgccaac ttttttcatc
    31741 tcatcaaggt attacgtgcc aataagtatg accttatcgt caatcttacc gatcaatgga
    31801 tggttgctat actggttcgc ttattaaatg cccgtgtgaa aatttcccag gattatcatc
    31861 atcggcagtc tgctttttgg cgtaaaagtt tcacccattt ggtgccgttg cagggtggaa
    31921 atgtggtgga aagtaactta tccgtgctga caccattggg acttgattcg ttggtgaagc
    31981 agacaaccat gagttacccg cctgcaagct ggaaacgtat gcgtcgcgaa cttgatcacg
    32041 ctggtgttgg acaaaattat gtggttatcc aacctacggc gcggcaaatc ttcaaatgct
    32101 gggacaacgc caagttttcc gctgtgattg atgccttaca tgctcgtggt tatgaagtcg
    32161 ttctgacgtc cggcccggat aaagacgatc tggcctgcgt caatgaaatt gcgcagggat
    32221 gccagacgcc accagtaacg gcgctggctg gaaaggtgac cttcccggaa cttggtgcgt
    32281 taatcgatca tgcgcagctg tttattggcg ttgattccgc accggcgcat attgccgctg
    32341 cagttaatac gccgctgata tcgctgtttg gtgcgacaga ccatattttc tggcgtccct
    32401 ggtcaaataa catgattcaa ttctgggcgg gagattaccg ggaaatgcca acgcgcgatc
    32461 agcgtgaccg aaatgagatg tatctttcgg ttattccggc ggcagatgtc attgctgctg
    32521 tcgataaatt actgccctcc tccacgacag gtacgtcgtt atgatcgttg ctttttgttt
    32581 atataaatat tttccctttg gcggtttgca gcgtgatttt atgcgtattg ctcagacagt
    32641 cgccgcccga ggtcatcatg ttcgggttta tacccagtcg tgggaaggcg aatgccctga
    32701 tgtatttgaa ctgatcaaag tgccggttaa atcgcatacc aatcacgggc gcaatgcgga
    32761 gtattttgcc tgggtgcaaa aacatttacg cgaacatccc gtcgataaag tcgttggatt
    32821 caacaaaatg ccggggctgg acgtttatta tgccgctgat gtttgttatg ccgagaaagt
    32881 agcgcaggaa aaaggctttt tctatcgcct gacgtcacgt tatcgccatt atgccgcctt
    32941 tgagcgggca accttcgaac agggtaagcc gacacagttg ctgatgctga cagataagca
    33001 aatcgccgat ttccagaaac attatcagac tgaagcggag cgttttcata ttctgccacc
    33061 ggggatttat cctgatcgta aatatagcca gcagccagcc aatagccgtg aaatcttccg
    33121 taagaagaat ggaataaccg aacaacaata tttattgttg caggtcggtt cagacttcac
    33181 gcgtaaaggt gtcgatcgtt ccattgaagc acttgcttcg ttaccggatt cgctgcgcca
    33241 caacacattg ctatatgttg ttgggcagga taaaccgcga aaatttgagg cgctggcaga
    33301 aaaacgcggc gtgcgcagta atgttcactt tttctcgggg cgcaatgatg tctcggaatt
    33361 aatggcggcg gcggatttat tactgcatcc tgcctaccag gaagcggcgg gaattgtgct
    33421 gctggaagcg attactgcag gattaccggt actaacaaca gccgtttgtg gctatgcgca
    33481 ttatattgtc gacgccaatt gcggcgaggc tattgctgag ccattccgcc aggaaacatt
    33541 gaatgagatt ttacgcaaag cgttaacgca atcttcattg cgccaggcct gggcggaaaa
    33601 tgcgcgacat tatgctgata cacaagattt atacagtctg ccagagaaag cggcggatat
    33661 cataacgggt ggtctggatg gttgaactta aagagccgtt tgccacgtta tggcgcggca
    33721 aagatccttt tgaggaagtt aaaaccttgc agggtgaggt atttcgtgaa ctggaaactc
    33781 gccgtactct gcgctttgaa atggcgggca aaagctattt tctcaaatgg catcgcggca
    33841 cgaccctgaa agagataatc aaaaatttac tctcattgcg gatgccagta ttaggcgcag
    33901 accgcgaatg gaatgcgatt catcgactgc gggatgtcgg cgttgatact atgtatgggg
    33961 tggcattcgg cgaaaaaggc atgaatccgc tgaccagaac ctcgtttatt attaccgaag
    34021 atctgacacc aaccataagt ctggaagatt actgtgctga ctgggcgact aaccctccag
    34081 atgttcgcgt aaagcgtatg cttattaagc gtgtcgcgac gatggtgcgc gatatgcatg
    34141 ctgcgggcat taaccaccgt gactgttata tctgtcattt cctgctgcac ttgccttttt
    34201 ccggtaagga agaggagtta aaaatttcgg taattgacct gcaccgggcg cagcttcgca
    34261 cgcgcgttcc acgtcgttgg cgggataaag atcttattgg gctttatttt tcttcgatga
    34321 atatcggcct gactcagcgg gatatctggc ggtttatgaa agtgtatttt gccgccccgc
    34381 ttaaagacat tctcaagcag gaacaaggac tgctgtcgca agcagaagca aaagccacaa
    34441 aaatcaggga aagaacgatt cgaaaatcgt tgtaatttcc ggagagaaat atgagtgccc
    34501 actattttaa tccacaagag atgatcacta agacaatcat cttcgatgaa aggccagcgg
    34561 cgtcagtggc atcatcattc catgttgctt atggcattga taaaaacttt ctttttggtt
    34621 gtggtgtttc aatcacgtca gttttgttac ataacaacga cgtgagtttt gttttccacg
    34681 tttttattga tgatatccct gaagccgata tccagcgttt agcccaattg gcgaaaagct
    34741 atcgtacctg tatccagatc catctggtaa attgtgaacg gcttaaggca ttaccgacga
    34801 ccaaaaattg gtctattgcc atgtatttcc gttttgtaat tgcagattac tttattgatc
    34861 aacaagataa ggttctttac ctggatgctg atatcgcctg tcagggaaac ttaaagccgc
    34921 tgataacaat ggatcttgcc aataacattg ctgctgttgt tactgaacgc gatgctaact
    34981 ggtggtcgtt acggggtcaa agtctgcagt gtaatgaact tgaaaagggt tactttaatt
    35041 caggtgtcct gttaattaat acactagcgt gggcgcagga gtccgtttct gctaaagcga
    35101 tgtcgatgct tgctgataaa gccgtcgttt cccgtttaac ctatatggat caagatatac
    35161 ttaatcttat cctgtcaggg a
//
LOCUS       DAJOWB010000038        34397 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJOWB010000038 DAJOWB010000000
VERSION     DAJOWB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.038"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(134..472)
                     /locus_tag="OHO85_002035"
     CDS             complement(134..472)
                     /locus_tag="OHO85_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2671410.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLI
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKV"
     gene            842..1792
                     /gene="corA"
                     /locus_tag="OHO85_002036"
     CDS             842..1792
                     /gene="corA"
                     /locus_tag="OHO85_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCQ2671411.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1835..2215)
                     /locus_tag="OHO85_002037"
     CDS             complement(1835..2215)
                     /locus_tag="OHO85_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCQ2671412.1"
                     /translation="MSKEYMNDGSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2229..2645)
                     /locus_tag="OHO85_002038"
     CDS             complement(2229..2645)
                     /locus_tag="OHO85_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2671413.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRRRYIFSFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIVLHYLVINMNCQRLRDSGFIYIKTYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2704..3594)
                     /gene="rarD"
                     /locus_tag="OHO85_002039"
     CDS             complement(2704..3594)
                     /gene="rarD"
                     /locus_tag="OHO85_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCQ2671414.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3646..4113)
                     /locus_tag="OHO85_002040"
     CDS             complement(3646..4113)
                     /locus_tag="OHO85_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCQ2671415.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4278..5147
                     /gene="pldA"
                     /locus_tag="OHO85_002041"
     CDS             4278..5147
                     /gene="pldA"
                     /locus_tag="OHO85_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCQ2671416.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5280..7109
                     /gene="recQ"
                     /locus_tag="OHO85_002042"
     CDS             5280..7109
                     /gene="recQ"
                     /locus_tag="OHO85_002042"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCQ2671417.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7173..7793
                     /gene="rhtC"
                     /locus_tag="OHO85_002043"
     CDS             7173..7793
                     /gene="rhtC"
                     /locus_tag="OHO85_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCQ2671418.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7855..8475)
                     /gene="rhtB"
                     /locus_tag="OHO85_002044"
     CDS             complement(7855..8475)
                     /gene="rhtB"
                     /locus_tag="OHO85_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCQ2671419.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQMMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8586..9608
                     /gene="pldB"
                     /locus_tag="OHO85_002045"
     CDS             8586..9608
                     /gene="pldB"
                     /locus_tag="OHO85_002045"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCQ2671420.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9616..10416
                     /gene="yigL"
                     /locus_tag="OHO85_002046"
     CDS             9616..10416
                     /gene="yigL"
                     /locus_tag="OHO85_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCQ2671421.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTAHGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10492..11391
                     /gene="bioP"
                     /locus_tag="OHO85_002047"
     CDS             10492..11391
                     /gene="bioP"
                     /locus_tag="OHO85_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCQ2671422.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11279..12232)
                     /gene="metR"
                     /locus_tag="OHO85_002048"
     CDS             complement(11279..12232)
                     /gene="metR"
                     /locus_tag="OHO85_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCQ2671423.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12350..14611
                     /gene="metE"
                     /locus_tag="OHO85_002049"
     CDS             12350..14611
                     /gene="metE"
                     /locus_tag="OHO85_002049"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCQ2671424.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14650..15465)
                     /locus_tag="OHO85_002050"
     CDS             complement(14650..15465)
                     /locus_tag="OHO85_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCQ2671425.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15727..16488
                     /gene="udp"
                     /locus_tag="OHO85_002051"
     CDS             15727..16488
                     /gene="udp"
                     /locus_tag="OHO85_002051"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCQ2671426.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            16629..18056
                     /gene="rmuC"
                     /locus_tag="OHO85_002052"
     CDS             16629..18056
                     /gene="rmuC"
                     /locus_tag="OHO85_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCQ2671427.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQYAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18151..18906
                     /gene="ubiE"
                     /locus_tag="OHO85_002053"
     CDS             18151..18906
                     /gene="ubiE"
                     /locus_tag="OHO85_002053"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCQ2671428.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18920..19525
                     /gene="ubiJ"
                     /locus_tag="OHO85_002054"
     CDS             18920..19525
                     /gene="ubiJ"
                     /locus_tag="OHO85_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCQ2671429.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19522..21162
                     /gene="ubiB"
                     /locus_tag="OHO85_002055"
     CDS             19522..21162
                     /gene="ubiB"
                     /locus_tag="OHO85_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCQ2671430.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21241..21510
                     /gene="tatA"
                     /locus_tag="OHO85_002056"
     CDS             21241..21510
                     /gene="tatA"
                     /locus_tag="OHO85_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCQ2671431.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21514..22029
                     /gene="tatB"
                     /locus_tag="OHO85_002057"
     CDS             21514..22029
                     /gene="tatB"
                     /locus_tag="OHO85_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCQ2671432.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22032..22808
                     /gene="tatC"
                     /locus_tag="OHO85_002058"
     CDS             22032..22808
                     /gene="tatC"
                     /locus_tag="OHO85_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCQ2671433.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22850..23632
                     /gene="tatD"
                     /locus_tag="OHO85_002059"
     CDS             22850..23632
                     /gene="tatD"
                     /locus_tag="OHO85_002059"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCQ2671434.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23629..24117)
                     /gene="rfaH"
                     /locus_tag="OHO85_002060"
     CDS             complement(23629..24117)
                     /gene="rfaH"
                     /locus_tag="OHO85_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCQ2671435.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24284..25777
                     /gene="ubiD"
                     /locus_tag="OHO85_002061"
     CDS             24284..25777
                     /gene="ubiD"
                     /locus_tag="OHO85_002061"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCQ2671436.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25823..26524
                     /gene="fre"
                     /locus_tag="OHO85_002062"
     CDS             25823..26524
                     /gene="fre"
                     /locus_tag="OHO85_002062"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCQ2671437.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26708..27871)
                     /gene="fadA"
                     /locus_tag="OHO85_002063"
     CDS             complement(26708..27871)
                     /gene="fadA"
                     /locus_tag="OHO85_002063"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCQ2671438.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27881..30070)
                     /gene="fadB"
                     /locus_tag="OHO85_002064"
     CDS             complement(27881..30070)
                     /gene="fadB"
                     /locus_tag="OHO85_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCQ2671439.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLQFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFRE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30260..31591
                     /gene="pepQ"
                     /locus_tag="OHO85_002065"
     CDS             30260..31591
                     /gene="pepQ"
                     /locus_tag="OHO85_002065"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCQ2671440.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31591..32205
                     /locus_tag="OHO85_002066"
     CDS             31591..32205
                     /locus_tag="OHO85_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCQ2671441.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32244..33695
                     /gene="trkH"
                     /locus_tag="OHO85_002067"
     CDS             32244..33695
                     /gene="trkH"
                     /locus_tag="OHO85_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCQ2671442.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33707..34252
                     /gene="hemG"
                     /locus_tag="OHO85_002068"
     CDS             33707..34252
                     /gene="hemG"
                     /locus_tag="OHO85_002068"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCQ2671443.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8085 a         8715 c         9306 g         8291 t
ORIGIN      
        1 cttgcaagta tgggacaact tgtgaaatta ccgacaagaa gggaaagggc cttcccgaga
       61 gtggtaaagg agaggccctg gaaatacccc acagccccga aaaagagcca gtcagttgct
      121 taactgactg gcattacacc ttctgctgac cgttttcgat gtacaaaccg agcggcgcat
      181 agctttcatc atagatgccg ccgttcatcg ccatctgcac ctgaccctga ctattcatat
      241 ccaccagcag agcatgtaaa gttccccacg cttcgccatt ggctttttgc cagtacattt
      301 tcacccgctc tgtttgagga ttaacggtat acgcctgtac gatcagcatc ggatctgaga
      361 gtgcgcaatc atcagcggca acagcaaaca agggaagtaa ggtgagggcg agaaaaatcc
      421 gtttgagatt caaggtgatc attcctttac caatgagtag ccgatgcgcc attataggtc
      481 ctggatgtgg gattttttta tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt
      541 tcaactattg ttctgtggtg ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca
      601 tgcgcgcacg atcactctaa gaggacattc gccttggaca cacccagtag atactggctc
      661 actatcctgt catccaggat caactcctaa ggctatccct ttttgctgat agccttagcg
      721 gttgtcagcg acctcaattt ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac
      781 ccaatttgtt tctgtgtgcc caccgaactg tccgatattt taagcattgg gagtcccggt
      841 catgctgagc gcatttcaac tggaaaataa ccgactgacc cggctggaag tcgaagagtc
      901 acaacccctt gtaaatgcag tatggattga tcttgtcgaa ccggacgacg acgagcgact
      961 gcgcgtacaa tctgaacttg gccagagcct ggcaacccgc ccggaactgg aagacatcga
     1021 agcatcggca cgtttctttg aagacgacga cggcctgcat attcactcct tcttcttctt
     1081 tgaagatgcg gaagatcacg ccggtaactc cactgtggca tttaccatcc gtgatggtcg
     1141 tctgtttact ctgcgtgagc gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg
     1201 tagccagtcg atggtagacg gtaacgccta cgagctgctg ctggatctgt tcgaaaccaa
     1261 aatcgaacag ttggcagatg aaattgaaaa tatctatagc gacctggagc agttgagccg
     1321 ggtgattatg gaagggcatc agggcgatga gtacgacgag gcgctctcca ctctggcgga
     1381 actggaagat atcggctgga aagtgcgcct gtgtctgatg gatacccagc gtgcgctcaa
     1441 cttcctggtg cgtaaagcgc gtttaccggg tgggcaactg gagcaggcgc gtgaaatcct
     1501 gcgagatatc gaatccctgc tgccgcataa cgaatccctg ttccagaagg tgaacttcct
     1561 gatgcaggcg gcaatgggtt ttatcaacat cgagcagaac cgcatcatca aaattttctc
     1621 ggtggtatcc gtcgtgttcc tgccgccaac gctggttgct tccagctatg ggatgaactt
     1681 tgagtttatg ccagaactga agtggagttt cggctaccct ggcgcgatta tctttatgat
     1741 cctcgcgggc ctggcaccgt atctgtactt taagcggaag aactggttgt aaaaaacgag
     1801 agcggtggct tagtctggct aagccacctg ttattcaaag gctccaggta tttaaccctt
     1861 ttacctcttt ctcatagaac catttgttcg tgttaacagc aacataggct gctacggcaa
     1921 ttcccagaat gttaacgcca attaatgcac caacaaatag agatagaatg ccaagtaata
     1981 gaactataat tgcttttttc cataagccca gaacaaataa atagatccag gaacagaaga
     2041 aagcaatgaa attcatttga atagttaatc gttgtcttac ttttaatgct ttaaatgctg
     2101 ctttatattc tggtgttgct ccccagaatc caggaaagcc atgttgatca taaaaattaa
     2161 atcggtattt ccatttttca cttaatgaac catcgttcat atattcctta ctcataaata
     2221 ctccataact attgttttga tgaatcagta ggtgcaagca ttagcatact gaaagtggaa
     2281 aaataacaaa tcaaaaaaat catcgaacca ttgcctgaac aggcaaaatc ttcggctatc
     2341 attgtgatga tagagatgat atatactgct aatgtaccaa aaacataagt ttttatatag
     2401 atgaaaccac tatcacggag tcgctggcaa ttcatgttga tgacgagata atggagtacg
     2461 atggtagaga ctataacaag aaaacctgct tccccatcgt taaaaaagat aataagaaag
     2521 gcaaaaatga aatttattaa aataaatgaa aatatatatc ggcgtctgga aatcttaccg
     2581 ttagatgttg ggataaacat acgtaacata aattttacat ccttgtgtga gtctcctgtc
     2641 agcatggcaa tatgcccact ctcatacaag agtcggcata ttttttcaga atatatttat
     2701 tttttatttg gacgttctac gctgcgtata aatcgcatcc atcacaaaaa ttgccagcgc
     2761 cacccaaata aaggcgaaag tcaccatctt atcggcaccc ggtttttcac cataaaacgt
     2821 cacagccagc aggaacatca gcgtcgggcc aatgtactgg aaaaagccta acgttgagag
     2881 acgcaggcgc gtggcagcgg cagtaaaaca caacagcggt acggtagtga caataccggc
     2941 ggcgatcagc agtaaattca gcgacatcgg gttttgcccc atatggctgg ttgagctgtc
     3001 ggcaatagca aacaggtaaa tcgccgccac gggcagcagc cacatggttt caattaacat
     3061 gccggtttgt gcttctaccg caatcttctt gcgcaccagg ccgtagaagg caaaactaaa
     3121 tgccagcccc agcgcgataa taggtagcga accaaaagtc cacagctgga ctaacacgcc
     3181 acatatcgcc agaatcaccg ccagccattg catccggcgg aatcgctcgc cgaggaaaat
     3241 catccccagc acaatgttca ccagcgggtt aataaagtaa ccaaggctcg cttccagcat
     3301 atggtgattg ttcaccgccc agataaacag tagccagttg ccaccaatca gcacggcaga
     3361 gactgccagc ataaaaattt tctgtggcgt ctgaatcagc gtttttaaat aggaccactg
     3421 gcggcaaatg ctcatcagca ccaccataaa gaaaaacgac cagatcacgc gatgcgtcag
     3481 gatttcatcg gcgggcacgt agtaaatcaa cttgaagtac gctggcgcta taccccaaat
     3541 aaaataagcg gcaagagcga gtaatacgcc ctgccgcgtt tgttttgcat ccatcgggaa
     3601 tactcatttt taattggtaa cagcagttta cctgctttta tgtcttcaac ctaccatata
     3661 ggtggcggtg gcactggcaa tataaagctg ttcttcattg tgtaattcaa cgcgggcgac
     3721 ggcgacttta ttgcctgcac gcaacaggct actggtagca gtaaaacgct cgcccctgcc
     3781 tgggcgcaga taatcaacgc gaagatcaat ggtccccatc cgcgatagcc gctggcgtag
     3841 ttcatcttca ctgatggttt cgtggcgggt taaggtactt cccacgcaca ccagaccggc
     3901 ggcgacatcc agcgccgacg caatgactcc gccgtgcaaa atgctttgcg cccagttacc
     3961 caccatcatc ggctgatttt taaaggccag ctgtgcgaac tctttttcgt aacgctccag
     4021 ctccatccct aacgcgcggt taaatggcat gtgataaaca aacatctcac ccactaattt
     4081 cagggcttgt tcagcggtca gtacggcaga catatcatcc ttatacttca ttggttaatg
     4141 aaatgttgat tttatgcttc tttgttgttg gtttctactt taggaaggga taactaacgg
     4201 caatggagtt aagtatgtaa aatagcccgc agaaaaacat tcaccttatc aataattcgt
     4261 tacggagaac acgaccgatg cggactctgc agggctggtt gttgccggtg tttatgttgc
     4321 ctatggcagt atatgcacaa gaggcaacgg tgaaagaggt gcatgacgcg ccagcggtgc
     4381 gtggcagtat tatcgccaat atgctgcagg agcatgacaa tccgttcacg ctctatcctt
     4441 atgacaccaa ctacctcatc tacacccaaa ccagcgatct gaacaaagaa gcgattgcca
     4501 gttacgactg ggcggaaaat gcgcgtaagg atgaagtaaa gtttcagttg agcctggcat
     4561 ttccactgtg gcgtgggatt ttaggcccga actcggtgtt gggtgcgtct tatacgcaaa
     4621 aatcctggtg gcaactgtcc aatagcgaag agtcttcacc gtttcgtgaa accaactacg
     4681 aaccgcaatt gttcctcggt tttgccaccg attaccgttt tgcaggttgg acgctgcgcg
     4741 atgtggagat ggggtataac cacgactcta acgggcgttc agacccgacc tcccgcagct
     4801 ggaaccgcct ttatactcgc ctgatggcag aaaacggtaa ctggctggta gaagtgaagc
     4861 cgtggtatgt ggtgggtaat actgacgata acccggatat caccaaatat atgggttact
     4921 accagcttaa aatcggctat cacctcggtg atgcggtgct cagtgcgaaa ggacagtaca
     4981 actggaacac cggctacggc ggcgcggagt taggcttaag ttacccgatc accaaacatg
     5041 tgcgccttta tactcaggtt tacagcggct atggcgaatc gctcatcgac tataacttca
     5101 accagacccg tgtcggtgtg ggggttatgc taaacgattt gttttgatga acggttgagt
     5161 ggttggcaaa tctggaatcc agcatccagg attaccctct cagagactaa aagcattgca
     5221 gtttctcgcg caggcgctga aaatagcgcc tgtttttatt tcaggcaatc ggggtgaatg
     5281 tggcgcaggc ggaagtgttg aatctggagt ccggagctaa acaggtttta caagaaacct
     5341 ttggctacca acagtttcgc cccggccagg aagaaattat cgacactgtg ctttccggtc
     5401 gcgattgcct ggtcgtcatg cccaccggtg gcggaaaatc cctttgctat caaattcctg
     5461 ccttattgct aaacggcctt accgtggttg tttcaccgct gatttcgttg atgaaagatc
     5521 aggtggatca actgcaagcc aacggcgtgg cggcggcgtg ccttaactcg acgcaaaccc
     5581 gcgagcagca acttgaagtg atgacaggct gtcgcaccgg gcaaatccgc ctgctttata
     5641 tcgcgccgga acgcctgatg ctggataact ttcttgagca tctggcgcac tggaatccgg
     5701 tgttattagc cgttgatgaa gcgcactgta tctcccaatg gggccacgat ttccgcccgg
     5761 aatatgccgc gctcggtcag ttgcgccagc ggttcccgac gctgccgttt atggcgctga
     5821 ccgccacagc cgacgacacc acgcgccagg atatcgtgcg cctgctgggg ctgaacgatc
     5881 cgctgattca aatcagcagt tttgaccgtc cgaatattcg ctacatgctg atggagaaat
     5941 tcaaaccgct cgatcagttg atgcgctacg tgcaggaaca gcgcggtaag tccggcatta
     6001 tctactgcaa cagccgggcg aaagtagaag acaccgctgc gcgcctgcaa agcaagggta
     6061 ttagcgcggc ggcctatcat gccgggctgg aaaataatgt ccgcgccgac gtgcaggaga
     6121 aattccagcg cgatgacctg caaattgtgg tggcgacggt ggcgttcggc atgggcatca
     6181 ataaaccaaa cgttcgcttc gtggtccact ttgatattcc gcgcaatatc gaatcctatt
     6241 atcaggaaac cggtcgcgcc gggcgtgatg gcctgcctgc ggaagcgatg ctgttttacg
     6301 atccggctga tatggcgtgg ctgcgccgtt gtctggaaga gaagccgcag gggcagttgc
     6361 aggatatcga gcgccacaaa ctcaatgcga tgggcgcgtt tgccgaagcg caaacttgcc
     6421 gtcgtctggt attgctgaac tattttggcg aagggcgtca ggagccgtgc gggaactgcg
     6481 atatctgcct cgatccgccg aaacagtacg acggttcaac cgatgctcag attgcccttt
     6541 ccaccattgg tcgtgtgaat cagcggtttg ggatgggtta tgtggtggaa gtgattcgtg
     6601 gtgctaataa ccagcgtatc cgcgactatg gtcatgacaa actgaaagtc tatggcatgg
     6661 gccgtgataa aagccatgaa cattgggtga gcgtgatccg ccagctgatt cacctcggcc
     6721 tggtgacgca aaatattgcc cagcattctg ccctacaact gacagaggcc gcgcgcccgg
     6781 tgctgcgcgg cgaatcctct ttgcaacttg ccgtgccgcg tatcgtggcg ctcaaaccga
     6841 aagcgatgca gaaatcgttc ggcggcaact atgatcgcaa actgttcgcc aaattacgca
     6901 aactgcgtaa atcgattgcc gatgaaagca atgtcccgcc gtacgtggtg tttaacgacg
     6961 caaccttgat tgagatggct gaacagatgc cgatcaccgc cagcgaaatg ctcagcgtta
     7021 acggcgttgg gatgcgcaag ctggaacgct ttggtaaacc gtttatggcg cttatccgcg
     7081 cgcatgttga cggcgacgac gaagagtagt cagcggcata aaaaagtgcc aggatgaaga
     7141 ctccgtaaac gtttcccccg cgagtcaaat gtatgttgat gttatttctc accgtcgcca
     7201 tggtgcatat tgtggcgctt atgagccccg gtcccgattt cttttttgtc tctcagaccg
     7261 ctgtcagtcg ttcccgtaaa gaagcgatga tgggcgtgct gggcattacc tgcggtgtaa
     7321 tggtttgggc tgggattgcg ttgcttggcc tgcatttgat tatcgaaaaa atggcctggc
     7381 tgcatacgct gattatggtt ggcggtggcc tgtatctctg ctggatgggt taccagatgc
     7441 tacgcggtgc actgaaaaaa gaggctgtat ctgcgcctgc accacagatt gagctggcga
     7501 aaagcgggcg cagtttcctg aaaggtttac tgaccaatct cgctaatccg aaagcgatta
     7561 tctactttgg ctcggtgttc tcattgtttg tcggtgataa cgttggcact accgcgcgct
     7621 ggggcatttt tgcgctgatc attgtcgaaa cactggcgtg gtttaccgtc gttgccagcc
     7681 tgtttgccct gccgcaaatg cgccgtggtt atcaacgtct ggcgaagtgg attgatggtt
     7741 ttgccggggc gttatttgcc ggatttggca ttcatttgat tatttcgcgg tgatgccaga
     7801 cgcgtcttca gagtaagtcg gataaggcgt ttacgccgca tccgacatta tttttcacgc
     7861 atgcctcgcc gatgctaaca gcgctcccac cagcataaac aacgagccga aaatcttatt
     7921 cagcgccttc atctgctttg gtcctttaat ccatagagca atccgttgag caagggtggc
     7981 gtaaccgatc atcacaataa tatcgactac aatagtggtg acgccgagca cgatatactg
     8041 catcatttgc ggctgctgtg gcatgatgaa ttgcggaaat agcgccgcca gaaacacaat
     8101 acttttggga ttggtgagat tcacaaaaac tgcgcgctgg aacaaatgtc gacgcgattg
     8161 agtagaggcc agcgatttaa ggtcaattgc accagcggcg cgccactgct ggattcccag
     8221 ccaaatcaag taagccgcgc ctgcccactt caacacttca aacgcaatca ctgagcggga
     8281 aaatagcgtc cccaacccca cgccaaccag cacaatatga atcgccagtc cggtctgaag
     8341 cccagcaata gacgccaccg cgccgcgata accgtggttg agcgaggtgg tcatagtgtt
     8401 gattgcacca gagcctggcg acaggcttaa aatgatcgat gtcagcaggt aggcaaacca
     8461 ccattctaag gtcatgatga actcccggtg tgtctatttt tgtgccacaa tacgctactg
     8521 tcgcagcgtt gtgtcaggca cgctaaaaaa aacgatttta cgtggtttaa gaggcagatt
     8581 acccgatgtt tcagcagcaa aaagactggg aaacaagaga aaacgcgttt gctgctttta
     8641 ccatgggacc gctgactgat ttctggcgtc agcgtgatga agcagagttt actggtgtgg
     8701 atgacattcc ggtgcgcttt gtccgttttc gcgcacagca ccatgaccgg gtggtagtca
     8761 tctgcccggg gcgtattgag agctacgtaa aatatgcgga actggcctac gacctgttcc
     8821 atttggggtt tgatgtctta atcatcgacc atcgcgggca gggacgttcc ggtcgcctgt
     8881 tagccgatcc gcatctcggg catgttaatc gctttaatga ttatgttgat gatctggcgg
     8941 cattctggca gcaggaggtt cagcccggtc cgtggcgtaa acgctatata ctggcacatt
     9001 cgatgggcgg tgcgatctcc acattatttc tgcaacgcca tccaggtgta tgtgacgcca
     9061 ttgcgctaac tgcgccaatg tttgggatcg tgattcgtat gccgtcattt atggcacggc
     9121 agatcctcaa ctgggccgaa gcgcatccac gtttccgtga tggctatgca ataggcaccg
     9181 ggcgctggcg cgcgttgccg tttgctatca acgtactgac ccacagcaga cagcgatatc
     9241 gacgtaactt acgcttctat gctgatgacc caacgattcg cgtcggtggg ccgacctacc
     9301 attgggtacg cgaaagtatt ctggctggcg aacaggtgtt agccggtgcg ggtgatgacg
     9361 ccacgccaac gcttctcttg caggctgaag aggaacgcgt ggtggataac cgcatgcatg
     9421 accgtttttg tgaactccgc accgccgcgg gccatcctgt cgaaggagga cgaccgttgg
     9481 taattaaagg tgcttaccat gagatccttt ttgaaaagga cgcaatgcgc tcagtcgcgc
     9541 tccacgccat tgttgatttt ttcaacaggc ataactcacc cagcggaaac cgctctacag
     9601 aggtttaaat ttcttatgta ccaggttgtt gcgtctgatt tagatggcac gttactttct
     9661 cccgaccata cgttatcccc ttacgccaaa gaaaccctga agctgctcac cgcgcacggc
     9721 atcaactttg tgtttgcgac cggtcgtcac cacgttgatg tggggcaaat tcgcgataat
     9781 ctggagatta agtcttacat gattacctcc aacggcgcgc gcgttcacga tctggatggc
     9841 aatctgattt ttgctcataa cctggatcgc gacattgcca gcgatctgtt tggcgtagtc
     9901 aacgacaatc cggacatcat tactaacgtt tatcgcgacg acgaatggtt tatgaatcgc
     9961 catcgcccgg aagagatgcg cttttttaaa gaagcggtgt tcaaatatgc gctgtatgag
    10021 cctggattac tggagccgga aggcgtcagc aaagtgtttt tcacctgcga ttcccatgaa
    10081 caactgctgc cgctggagca ggcgattaac gctcgttggg gcgatcgcgt caacgttagt
    10141 ttctctacct taacctgtct ggaagtgatg gcgggcggcg tttcaaaagg ccatgcgctg
    10201 gaagcggtgg cgaagaaact gggctacagc ctgaaggatt gtattgcgtt tggtgacggg
    10261 atgaacgatg ccgaaatgct gtcgatggcg gggaaaggct gcattatggg cagtgcgcac
    10321 cagcgtctga aagaccttca tcccgagctg gaagtgattg gtactaatgc cgacgacgcg
    10381 gtgccgcatt atctgcgtaa actctattta tcgtaatcgt tctttatttg gtcagttgtc
    10441 aacctgatac ttcgctacaa tggatactcg ttaatcaaag agttttccat tgtggcgcta
    10501 cttatcatca ccacgattct gtgggccttc tcctttagct tttatggcga gtaccttgcg
    10561 gggcacgtcg atagctattt tgcggtgctg gtgcgcgttg gcctggcggc actcgttttt
    10621 ctgccgtttc tgcgtacccg tggcaatagc ctgaaaacag tcggcctgta tatgctggtg
    10681 ggcgcgatgc agctcggcgt gatgtatatg ctgagtttcc gcgcttatct ctacctgacg
    10741 gtttccgaac tgctgctgtt caccgtactg acgccgctct acatcacgct gatttatgac
    10801 atcatgagta agcgccgtct gcgctgggga tatgctttta gtgccttgtt ggcggtgatt
    10861 ggtgccggga ttattcgcta tgatcaggtc accgaccatt tctggactgg cttgttgctg
    10921 gtgcaactct ccaatatcac ttttgccatc ggcatggtgg gttacaaacg cctgatggaa
    10981 actcgcccga tgccgcagca taacgccttt gcgtggttct atcttggcgc gttcctggtg
    11041 gcggtaattg catggttctt gctgggaaat gcgcagaaaa tgccgcaaac cacgttgcaa
    11101 tggggcattc tggtgtttct tggcgtggtg gcttccggga ttggctactt tatgtggaac
    11161 tacggcgcga cgcaggtgga cgccggaacg ctgggcatta tgaataatat gcacgttccg
    11221 gcagggctgc tggtaaacct ggctatctgg caccaacagc cgcactggcc aacgttcatt
    11281 acaggcgcgc tggtgatcct ggcctcactg tgggtgcatc gtaagtgggt cgctccgcgc
    11341 tcttcacaaa cggcagatga tcgcaggcgt gattgcgcgc tgagcgaata aatgcttctg
    11401 taatcggctg acgctgctcg ccatcgcgca cggcggcgta cagtcggctc cacaagcctt
    11461 cgcccagggt tttggtcacc accagaccct ggcgctcaaa actctctact acccaatgcg
    11521 gtagcgcggc aatacccatc cgcgcggcaa ccatctgaat caacaataag gtgttatcga
    11581 cgcttttcag tgacgggctg acgcctgccg gctgaagaaa atgccgccag acatccagtc
    11641 gactacgctg caccggataa atcaacagcg tctcgctggc gagatcttcc ggtgtaattc
    11701 gcgttttcgc cgccagtgga tggtcaggtg ctaacaccag acgcacttca tagtcgaaca
    11761 tcggcgaata atgcaggcca ctgcgcggca ggatatcgga ggtcatcacc agatccagct
    11821 cgccctgttg caacgcgggc tgcgggtcaa acgtcacgcc cgatttaaaa tccatctcta
    11881 cctgcggcca gttcttatgg aaattttcta acgcgggcgt cagccactga atacagctat
    11941 ggcactcaat ggcaatgcgc agacgcgtct gctgcggttc attgcaggct tgcagtgcct
    12001 ggctaatttg cggcagcacc tggtttgcca gttgcaacag gatttctccc tgcggtgtaa
    12061 agcgtagcgg ctggctctta cgcacaaata gccggaagcc aaggcgttgt tccagatcgc
    12121 taaactggtg agacagggcg gattgcgtct gatgcaacgt cgccgcagcg gctgcgagag
    12181 agccgcagtt ccgcaacgct tgtagcgttt tcaggtgttt tacttcgatc atgaaagtcc
    12241 ttcacttcgg catgaataat ttgcgcttga ggaatataca gtaaccgcca attatggatg
    12301 tgtaaacatc tggacggcta aatactaaaa attccataag gggcatataa tgacaattct
    12361 taatcacacc ctcggtttcc ctcgcgttgg cctgcgtcgc gagctgaaaa aagcgcaaga
    12421 aagttattgg gcggggaact ccacgcgtga agaactgctg acagtagggc gtgaactgcg
    12481 tgctcgtcac tgggatcaac aaaagcaagc gggtatcgac ctgctgccgg tgggcgattt
    12541 tgcctggtac gatcatgttc tgaccaccag tctgctgctg ggtaatgttc cgccacgtca
    12601 tcagaacaaa gacggttcgg tagatatcga caccctgttc cgtattggtc gtggacgtgc
    12661 gccgactggc gaacctgcgg cggcagcgga aatgaccaaa tggtttaaca ccaactatca
    12721 ctacatggtg ccggaattcg ttaaaggcca acagttcaaa ctgacctgga cgcagctgct
    12781 ggaggaagtg gacgaggcgc tggcgctggg ccacaacgtt aagcctgtgc tgctggggcc
    12841 ggttacttac ctgtggctgg ggaaagtgaa aggtgaacag tttgaccgcc ttagcctgct
    12901 gaacgacatt ctgccggttt atcagcaagt gctggcagaa ctggcgaaac gcggcatcga
    12961 gtgggtacag attgatgaac ccgcgctggt actggaacta ccacaggcgt ggctggacgc
    13021 atacaaaccc gcttacgacg cgctccaggg acaggtgaaa ctgctgctga ccacctattt
    13081 tgaaggcgta acgccaaatc tcgacacgat tactgcgctg cctgttcagg gtctgcatgt
    13141 tgaccttgta catggtaaag atgacgttgt tgaactgcac aagcgcctgc cttctgactg
    13201 gctgctgtct gcgggactga tcaatggtcg taacgtctgg cgcgccgatc ttactgagaa
    13261 atatgcgcaa attaaggaca ttgtcggcaa acgtgatttg tgggtggcat cttcctgctc
    13321 gttgctgcac agccccatcg acctgagcgt ggaaacgcgt cttgatgcag aagtgaaaag
    13381 ctggtttgcc ttcgctctac aaaaatgcca tgaactggca ctgctgcgcg atgcgctgaa
    13441 cagtggtgac acggcagctc tggcagagtg gagcgcccca attcaggcgc gtcgtcactc
    13501 tactcgtgta cataatccgg cggtagaaaa gcgtctggcg gcgatcaccg cccaggacag
    13561 ccagcgtgcg aatgtctatg aagtgcgtgc tgaagcccag cgtgcgcgtt ttaaactgcc
    13621 agcgtggccg accaccacga ttggttcctt cccgcaaacc acggaaattc gtaccctgcg
    13681 tctggatttc aaaaagggca atctcgacgc caacaactac cgcacgggca ttgcggaaca
    13741 tatcaagcag gccattgttg agcaggaacg tttgggactg gatgtgctgg tacatggcga
    13801 ggccgagcgt aatgacatgg tggaatactt tggcgagcac ctcgacggat ttgtctttac
    13861 gcaaaacggt tgggtacaga gctacggttc ccgctgcgtg aagccaccga ttgtcattgg
    13921 tgacattagc cgcccggcac cgattaccgt ggagtgggcg aagtatgcgc aatcgctgac
    13981 cgacaaaccg gtgaaaggga tgctgacggg gccggtgacc atactctgct ggtcgttccc
    14041 gcgtgaagat gtcagtcgtg aaaccatcgc caaacagatt gcgctggcgc tgcgtgatga
    14101 ggtggccgat ctggaagcgg cagggattgg catcatccag attgatgaac cggcgctgcg
    14161 cgaaggttta ccgctgcgtc gtagcgactg ggacgcctat ctccagtggg gcgtagaggc
    14221 cttccgtatc aacgccgccg tggcgaaaga tgacacacaa atccacactc acatgtgtta
    14281 ttgcgagttc aacgacatca tggattcgat tgcggcgctg gacgcagacg tcatcaccat
    14341 cgaaacctcg cgttccgaca tggagttgct ggagtcgttt gaagagtttg attatccaaa
    14401 tgaaatcggt cctggcgtct atgacattca ctcgccaaac gtaccgagcg tggaatggat
    14461 tgaagccttg ctgaagaaag cggcaaaacg cattccggca gagcgcctgt gggtcaaccc
    14521 ggactgtggc ctgaaaacgc gcggctggcc agaaacccgc gcggcactgg cgaacatggt
    14581 gcaggcggcg cagaatttgc gtcgggggta aatccaaacc gggtggtaat accacccggt
    14641 cttttctcat tacagcgact tcttcccacc atactgctta aaccactcca gcatacgctg
    14701 ccagccatct ttcgcagatt cggcatgata gctcgggcga taatcggcgt tgaatgcatg
    14761 cccggcatcc gggtagacga taatctctgc tttcgcatta gcagcccgca gcgcctggcg
    14821 catggtttca acgctctcct gcggaatgct gttatcctga ccaccatata agccgagaac
    14881 cggcgcgtta agatcggttg cgatatcaac aggttgtttc ggtgaattca gcgacttgtc
    14941 gcccgtcagt ttgccgtacc acgccactgc agcttttagc tgtggattat gcgcggcata
    15001 cagccaggtg atacgtccac cccagcagaa tccggtgatc attaaacgat gaacatcgcc
    15061 gccgttgcgg gaagcccaac tggcgacatg atcgagatcg gccagcacct gcgagtcagg
    15121 cacttttgct accagaccgc taagcaacgt ggggatatcg gcaaaatcat tcggatcgcc
    15181 ttcgcggaag taaagttcag gtgcgatagc cagatacccc tccagcgcca gacggcgaca
    15241 aatgtcgcgg atatgttcat gcacgccaaa aatttcctgc actacaatga ccactggcag
    15301 tgggccatcg ctttgctttg gtctggcatg gtaagcaggc atgttatccc cttgtgaagg
    15361 gatagaggtg aagcccgcca caatcgcgtc gtccggggtc tgaacgatgg tcgaagcgag
    15421 aggcgatgca gcaggtgcaa atccagattg ttgtgttgtt gccatggtat tctccgtacc
    15481 cttataaaaa tattgcgcaa tgttaactat agtcagcatg caacaaatca cattgcctga
    15541 atcggctcat cttttatgca gtcctgcaga atgaagagtg atttatgtga tttgaatcac
    15601 ttttggtggg taaatttatg caacgcattt gcgtcatggt gatgagtatc acgaaaaaat
    15661 gttaaaccct tcggtaaagt gtctttttgc ttcttctgac taaaccgatt cacagaggag
    15721 ttgtatatgt ccaagtctga tgtttttcat ctcggcctca ctaaaaacga tttacaaggg
    15781 gctacgcttg ccatcgtccc tggcgacccg gatcgtgtgg aaaagatcgc cgcgctgatg
    15841 gataagccgg ttaagctggc atctcaccgc gaattcacta cctggcgtgc agagctggat
    15901 ggtaaacctg ttatcgtctg ctctaccggt atcggcggcc cgtctacctc tattgctgtt
    15961 gaagagctgg cacagctggg cattcgcacc ttcctgcgta tcggtacaac gggcgctatt
    16021 cagccgcata ttaatgtggg tgatgtcctg gttaccacgg cgtctgtccg tctggatggc
    16081 gcgagcctgc acttcgcacc gctggaattc ccggctgtcg ctgatttcga atgtacgact
    16141 gcgctggttg aagctgcgaa atccattggc gcgacaactc acgttggcgt gacagcttct
    16201 tctgatacct tctacccagg tcaggaacgt tacgatactt actctggtcg cgtagttcgt
    16261 cactttaaag gttctatgga agagtggcag gcgatgggcg taatgaacta tgaaatggaa
    16321 tctgcaaccc tgctgaccat gtgtgcaagt cagggcctgc gtgccggtat ggtagcgggt
    16381 gttatcgtta accgcaccca gcaagagatc ccgaatgctg agacgatgaa acaaactgaa
    16441 agccaagcgg tgaaaatcgt ggtggaagcg gcgcgtcgtc tgctgtaatt ctcttctcct
    16501 gtctgaaggc cgacgcgttc ggccttttgt atttttgcgt agcgcctcgc aggaaatgcc
    16561 tttccaactg gacgtttgta cagcacaatt ctattttgtg cgggtaagtt gttgcgtcag
    16621 gaggcgttgt ggatttctca atcatggttt acgcagttat tgcgttggtg ggtgtggcaa
    16681 ttggctggct gtttgccagt tatcaatatg cgcagcaaaa agccgagcaa ttagctgaac
    16741 gtgaagagat ggtcgcggag ttaagcgcgg caaaacaaca aattacccaa agcgagcact
    16801 ggcgtgcaga gtgcgagtta ctcaataacg aagtgcgcag cctgcaaagt attaacacct
    16861 ctctggaggc cgatctgcgt gaagtaacca cgcggatgga agccgcacag caacatgctg
    16921 acgataaaat tcgccagatg attaacagcg agcagcgcct cagtgagcag tttgaaaacc
    16981 tcgccaaccg tatttttgag cacagcaatc gccgggttga tgagcaaaac cgtcagagtc
    17041 tgaacagcct gttgtcgccg ctacgtgaac aactggacgg tttccgccgt caggttcagg
    17101 acagcttcgg taaagaagca caagaacgcc ataccctgac ccacgaaatt cgcaatctcc
    17161 agcaactcaa cgcgcaaatg gcccaggaag cgatcaacct gacgcgcgcg ctgaaaggcg
    17221 acaataaaac ccagggcaac tggggcgagg tagtattgac gcgggtgctg gaggcttccg
    17281 gtctgcgtga agggtatgaa tatgaaaccc aggtcagcat cgaaaatgac gcccgctcgc
    17341 ggatgcagcc ggatgtcatc gtgcggctgc cgcagggaaa agatgtggtg atcgacgcca
    17401 aaatgacgct ggtcgcctat gaacgctatt ttaacgccga agacgactac acccgcgaaa
    17461 gcgcgctaca ggaacatatc gcgtcggtgc gtaaccatat ccgtttgctg ggacgcaaag
    17521 attatcaaca gctgccgggg ctgcgaactc tggattacgt gctgatgttt attcccgttg
    17581 aacccgcttt tttactggcg cttgaccgcc agccggagct gatcaccgaa gcgttgaaaa
    17641 acaatatcat gctggttagc ccgactacgc tgctggtggc gttgcgtact attgccaacc
    17701 tgtggcgtta tgagcatcaa agccgcaacg cccagcaaat cgccgatcgt gccagcaagc
    17761 tgtacgacaa gatgcgtttg ttcatcgatg acatgtccgc gattggtcaa agtctcgaca
    17821 aagcgcagga taactaccgc caggcaatga aaaaactctc ttcaggtcgc ggaaatgtgc
    17881 tggcgcaggc agaagcgttt cgcggtttag gagtagaaat taaacgcgag attaatccgg
    17941 atttggctga acaggcggtg agccaggatg aagagtatcg acttcggtcg gttccggagc
    18001 agccgaatga tgaagcttat caacgcgatg atgaatataa tcagcagtcg cgctagccca
    18061 ttaggggtag ttaagccggg tagaaatcta gggcatcgac gcccaatctg ttacacttct
    18121 ggaacaattt tttgatgagc aggcattgag atggtggata agtcacaaga aacgacgcac
    18181 tttggttttc agaccgtcgc gaaggaacaa aaagcggata tggtcgccca cgttttccat
    18241 tccgtggcat caaaatacga tgtcatgaat gatttgatgt catttggtat tcatcgtttg
    18301 tggaagcgat tcacgattga ttgcagcggc gtacgccgtg ggcagaccgt gctggatctg
    18361 gctggtggca ccggcgacct gacagcgaaa ttctcccgcc tggtcggaga aactggcaaa
    18421 gtggtccttg ctgatatcaa tgaatccatg ctcaaaatgg gccgcgagaa gctgcgtaat
    18481 atcggtgtga ttggcaacgt tgagtatgtt caggcgaacg ctgaggcgct gccgttcccg
    18541 gataacacct ttgattgcat caccatttcg tttggtctgc gtaacgtcac cgacaaagat
    18601 aaagcactgc gttcaatgta tcgcgtgctg aaacccggcg gccgcctgct ggtgcttgag
    18661 ttctcgaagc caattatcga gccgctgagc aaagcctatg atgcatactc cttccatgtg
    18721 ctgccgcgta ttggctcact ggtcgcgaac gacgccgaca gctaccgtta tctggcagaa
    18781 tccatccgta tgcatcccga tcaggatacc ctgaaagcca tgatgcagga tgccggattc
    18841 gaaagtgtcg actactacaa tctgacggca ggggttgtgg cgctgcatcg tggttataag
    18901 ttctgacagg agaccggaaa tgccttttaa acctttagtg acggcaggaa ttgaaagtct
    18961 gctcaacacc ttcctgtatc gctcacccgc gctgaaaacg gcccgctcgc gtctgctggg
    19021 taaagtattg cgcgtggagg taaaaggctt ttcgacgtca ttgattctgg tgttcagcga
    19081 acgccaggtt gatgtactgg gcgaatgggc aggcgatgct gactgcaccg ttatcgccta
    19141 cgccagtgtg ttgccgaaac ttcgcgatcg ccagcagctt accgcactga ttcgcagtgg
    19201 tgagctggaa gtgcagggcg atattcaggt ggtgcaaaac ttcgttgcgc tggcagatct
    19261 ggcagagttc gaccctgcgg aactgctggc cccttatacc ggtgatatcg ccgctgaagg
    19321 aatcagcaaa gccatgcgcg gaggcgcaaa gttcctgcat cacggcatta agcgccagca
    19381 acgttatgtg gcggaagcca ttactgaaga gtggcgtatg gcacccggtc cgcttgaagt
    19441 ggcctggttt gcggaagaga cggctgccgt cgagcgtgct gttgatgccc tgaccaaacg
    19501 gctggaaaaa ctggaggcta aatgacgcca ggtgaagtac ggcgcctata tttcatcatt
    19561 cgcacttttt taagctacgg acttgatgaa ctgatcccca aaatgcgtat caccctgccg
    19621 ctacggctat ggcgatactc attattctgg atgccaaatc ggcataaaga caaactttta
    19681 ggtgagcgac tacgactggc cctgcaagaa ctggggccgg tttggatcaa gttcgggcaa
    19741 atgttatcaa cccgccgcga tctttttcca ccgcatattg ccgatcagct ggcgttattg
    19801 caggacaaag ttgctccgtt tgatggcaag ctggcgaagc agcagattga agctgcaatg
    19861 ggcggcttgc cggtagaagc gtggtttgac gattttgaaa tcaagccgct ggcttctgct
    19921 tctatcgccc aggttcatac cgcgcgattg aaatcgaatg gtaaagaggt ggtgattaaa
    19981 gtcatccgcc cggatatttt gccggttatt aaagcggatc tgaaacttat ctaccgtctg
    20041 gctcgctggg tgccgcgttt gctgccggat ggtcgccgtc tgcgcccaac cgaagtggtg
    20101 cgcgagtacg aaaagacatt gattgatgaa ctgaatttgc tgcgggaatc tgccaacgcc
    20161 attcagcttc ggcgcaattt tgaagacagc ccgatgctct acatcccgga agtttaccct
    20221 gactattgta gtgaagggat gatggtgatg gagcgcattt acggcattcc ggtgtctgac
    20281 gttgcggcgc tggagaaaaa cggcactaac atgaaattgc tggcggaacg cggcgtgcag
    20341 gtgttcttca ctcaggtctt tcgcgacagc tttttccatg ccgatatgca ccctggcaac
    20401 atcttcgtaa gctatgaaca cccggaaaac ccgaaatata tcggcattga ttgcgggatt
    20461 gttggctcgc taaacaaaga agataaacgc tatctggcag aaaactttat cgccttcttt
    20521 aatcgcgact atcgcaaagt ggcagagcta cacgtcgatt caggctgggt gccaccagat
    20581 accaacgttg aagagttcga atttgccatt cgtacggtct gtgaacctat ctttgagaaa
    20641 ccgctggccg aaatttcgtt tggacatgta ctgttaaatc tgtttaatac ggcgcgtcgc
    20701 ttcaatatgg aagtgcagcc gcaactggtg ttactccaga aaaccctgct ctacgtcgaa
    20761 ggggtaggac gccagcttta tccgcaactc gatttatgga aaacggcgaa gcctttcctg
    20821 gagtcgtgga ttaaagatca ggtcggtatt cctgcgctgg tgagagcatt taaagaaaaa
    20881 gcgccgttct gggtcgaaaa aatgccagaa ctgcctgaac tggtttacga cagtttgcgc
    20941 cagggcaagt atttacagca cagtgttgat aagattgccc gcgagcttca gtcaaatcat
    21001 gtacgtcagg gacaatcgcg ttattttctc ggaattggcg ctacgttagt attaagtggc
    21061 acattcttgt tggtcagccg acctgaatgg gggctgatgc ccggctggtt aatggcaggt
    21121 ggtctgatcg cctggtttgt cggttggcgc aaaacacgct gattttttca tcgctcaagg
    21181 cgggccgtgt aacgtataat gcggccttgt ttaatcatca tctaccacag aggaacatgt
    21241 atgggtggta tcagtatttg gcagttattg attattgccg tcatcgttgt actgcttttt
    21301 ggcaccaaaa agctcggctc catcggttcc gatcttggtg cgtcgatcaa aggctttaaa
    21361 aaagcaatga gcgatgatga accaaagcag gataaaacca gtcaggatgc tgattttact
    21421 gcgaaaacta tcgccgataa gcaggcggat acgaatcagg aacaggctaa aacagaagac
    21481 gcgaagcgcc acgataaaga gcaggtgtaa tccgtgtttg atatcggttt tagcgaactg
    21541 ctattggtgt tcatcatcgg cctcgtcgtt ctggggccgc aacgactgcc tgtggcggta
    21601 aaaacggtag cgggctggat tcgcgcgttg cgttcactgg cgacaacggt gcagaacgaa
    21661 ctgacccagg agttaaaact ccaggagttt caggacagtc tgaaaaaggt tgaaaaggcg
    21721 agcctcacta acctgacacc cgaactgaaa gcgtcgatgg atgaactacg ccaggccgcg
    21781 gagtcgatga agcgttccta cgttgcaaac gatcctgaaa aggcgagcga tgaagcgcac
    21841 accatccata acccggtggt gaaagataat gaagctgcgc atgagggcgt aacgcctgcc
    21901 gctgcacaaa cgcaggccag ttcgccggaa cagaagccag aaaccacgcc agagccggtg
    21961 gtaaaacctg ctgcggacgc tgaaccgaaa accgctgcac cttccccttc gtcgagtgat
    22021 aaaccgtaaa catgtctgta gaagatactc aaccgcttat cacgcatctg attgagctgc
    22081 gtaagcgtct gctgaactgc attatcgcgg tgatcgtgat attcctgtgt ctggtctatt
    22141 tcgccaatga catctatcac ctggtatccg cgccattgat caagcagttg ccgcaaggtt
    22201 caacgatgat cgccaccgac gtggcctcgc cgttctttac gccgatcaag ctgaccttta
    22261 tggtgtcgct gattctgtca gcgccggtga ttctctatca ggtgtgggca tttatcgccc
    22321 cagcgctgta taagcatgaa cgtcgcctgg tggtgccgct gctggtttcc agctctctgc
    22381 tgttttatat cggcatggca ttcgcctact ttgtggtctt tccgctggca tttggcttcc
    22441 ttgccaatac cgcgccggaa ggggtgcagg tatccaccga catcgccagc tatttaagct
    22501 tcgttatggc gctgtttatg gcgtttggtg tctcctttga agtgccggta gcaattgtgc
    22561 tgctgtgctg gatggggatt acctcgccag aagacttacg caaaaaacgc ccgtatgtgc
    22621 tggttggtgc attcgttgtc gggatgttgc tgacgccgcc ggatgtcttc tcgcaaacgc
    22681 tgttggcgat cccgatgtac tgtctgtttg aaatcggtgt cttcttctct cgcttttacg
    22741 ttggtaaagg gcgaaatcgg gaagaggaaa acgacgctga agcagaaagc gaaaaaactg
    22801 aagaataaat tcaaccgccc gtcagggcgg ttgtcatatg gagtacagga tgtttgatat
    22861 cggcgttaat ttgaccagtt cgcaatttgc gaaagaccgt gatgatgttg tagcgcgcgc
    22921 ttttgacgcg ggagttaatg ggctactcat caccggcact aacctgcgtg aaagccagca
    22981 ggcgcaaaag ctggcgcgtc agtattcgtc ctgttggtca acggcgggcg tacatcctca
    23041 cgacagcagc cagtggcaag ctgcgactga agaagcgatt attgagctgg ccgcgcagcc
    23101 agaagtggtg gcgattggtg aatgtggtct cgactttaac cgcaactttt cgacgccgga
    23161 agagcaggaa cgcgcttttg ttgcccagct acgcattgcc gcagatttaa acatgccggt
    23221 atttatgcac tgtcgcgatg cccacgagcg gtttatgaca ttgctggagc cgtggctgga
    23281 taaactgcct ggtgcggttc ttcattgctt taccggcaca cgcgaagaga tgcaggcgtg
    23341 cgtggcgcat ggaatttata tcggcattac cggttgggtt tgcgatgaac gacgcggact
    23401 ggagctgcgg gaacttttgc cgttgattcc ggcggaaaaa ttactgatcg aaactgatgc
    23461 gccgtatctg ctccctcgcg atctcacgcc aaagccatca tcccggcgca acgagccagc
    23521 ccatctgccc catattttgc aacgtattgc gcactggcgt ggagaagatg ccgcatggct
    23581 ggctgccacc acggatgcta atgtcaaaac actgtttggg attgcgtttt agagtttgcg
    23641 gaactcggtg ttcttcacac tgtgcttaat ctctttatta ataagattaa gcaatagcat
    23701 ggagcgagcc tcaccatcgg gttcggtgaa aatggcctga aagccttcga acgcgccttc
    23761 ggtaataatc accttatcac ccggataagg ggttgccgga tcgacaatgt ctttcggttt
    23821 atataccgat agctgatgaa tcaccgccga tgggactatc gctggcgacg cgccaaagcg
    23881 cacgaagtgg ctgacaccgc gggtcgcgtt gatagtcgtg gtatgaatca cttctggatc
    23941 aaattcgaca aacaggtagt tgggaaacaa tggctcactg actgcagtac gttttccacg
    24001 cacgattttt tccagggtga tcatcggtgc caggcaattc acagcctgtc tttcgaggtg
    24061 ttcctgggca cgttgaagtt gcccgcgctt gcagtacagt aaataccagg attgcataat
    24121 gactcttatc cgtttaatcg gggcgcaagg atagcaaaag ctttacgcta agttaattat
    24181 attccccggt ttgcgttata ccgtcagagt tcacgctaat ttaacaaatt tacagcatcg
    24241 caaagatgaa cgccgtataa tgggcgtaga ttaagaggct acaatggacg ccatgaaata
    24301 taacgattta cgcgacttcc tgacgctgct tgaacagcag ggtgagctaa aacgtatcac
    24361 gctcccggtg gatccgcatc tggaaatcac tgaaattgct gaccgcactt tgcgtgccgg
    24421 tgggcctgcg ctgttgttcg aaaaccctaa aggctactca atgccggtgc tgtgcaacct
    24481 gttcggtacg ccaaagcgcg tggcgatggg catggggcag gaagatgttt cggcgctgcg
    24541 tgaagttggt aaattattgg cgtttctgaa agagccggag ccgccaaaag gtttccgcga
    24601 cctgtttgat aaactgccgc agtttaagca agtattgaac atgccgacaa agcgactgcg
    24661 tggtgcgccc tgccaacaaa aaatcgtctc tggcgatgac gtcgatctca atcgcattcc
    24721 cattatgacc tgctggccgg aagatgccgc gccgctgatt acctgggggc tgaccgtaac
    24781 gcgcggcccg cataaagagc ggcagaatct gggcatttat cgtcagcagc tgattggtaa
    24841 aaacaaactg attatgcgct ggctgtcgca tcgcggcggc gcgctggatt atcaggagtg
    24901 gtgtgcggcg catccgggcg aacgtttccc ggtttctgtg gcgctgggtg ccgatcccgc
    24961 cacgattctc ggtgcagtca cccccgttcc ggatacgctt tcagagtatg cgtttgccgg
    25021 attgctacgt ggcaccaaga ccgaagtggt gaagtgtatc tccaatgacc ttgaagtgcc
    25081 cgccagtgcg gagattgtgc tggaagggta tatagaacaa ggcgaaactg ctccggaagg
    25141 gccgtatggc gaccacaccg gttactataa cgaagtcgat agtttcccgg tatttaccgt
    25201 gacgcatatt acccagcgtg aagatgcgat ttaccactcc acctatactg gtcgtccgcc
    25261 ggatgaaccc gcggtgctgg gtgtcgcact gaacgaagtg tttgtgccga ttctgcaaaa
    25321 acagttcccg gaaattgtcg atttttacct gccgccggaa ggctgttctt atcgtctggc
    25381 ggtagtgacg ataaaaaaac agtacgccgg acacgcgaag cgcgtcatga tgggcgtctg
    25441 gtcgttctta cgccagttta tgtacactaa gtttgtgatc gtttgcgatg atgacgtcaa
    25501 cgcacgcgac tggaacgatg tgatttgggc gattaccacc cgtatggatc cggcgcggga
    25561 tactgttctg gtagaaaata cgcctattga ttatctggat tttgcctcgc ctgtttccgg
    25621 gctgggttca aaaatggggc tggatgccac gaataaatgg ccgggggaaa cccagcgtga
    25681 atggggacgt cccatcaaaa aagatccaga tgttgtcgcg catattgacg ccatctggga
    25741 tgaactggct atttttaaca acggtaaaag cgcctgatgt gcgtttgttt tgccctattt
    25801 atcgatccga cagagaaagc gcatgacaac cttaagctgt aaagtgacct cggtagaagc
    25861 tatcacggat accgtatatc gtgtccgcat cgtgccagac gcggcctttt cttttcgtgc
    25921 tggtcagtat ttgatggtag tgatggatga gcgcgacaaa cgtccgttct caatggcctc
    25981 gacgccggat gaaaaagggt ttatcgagct gcatattggc gcttctgaaa tcaaccttta
    26041 cgcgaaagca gtcatggacc gcatcctcaa agatcatcaa atcgtggtcg atattcccca
    26101 cggagaagcg tggctgcgcg atgatgaaga gcgtccgatg attttgattg cgggcggcac
    26161 cgggttctct tatgcccgct cgattttgct gacagcgttg gcgcgtaacc caaaccgtga
    26221 tatcaccatt tactggggcg ggcgtgaaga gcagcatctg tatgatctct gcgagcttga
    26281 agcgctttcg ttaaagcacc ctggtctgca agtggtgccg gtggttgaac aaccggaagc
    26341 gggctggcgt gggcgtactg gcaccgtgtt aacggcggta ttgcaggatc acggtacgct
    26401 ggcagagcat gatatctata ttgccggacg ttttgagatg gcgaaaatag cccgtgacct
    26461 gttttgtagt gagcgtaatg cgcgggaaga tcgtctgttt ggcgatgcgt ttgcatttat
    26521 ctgagatata aaaaaacccg cccctgacag gcgggaagaa cggcaactaa aactttattc
    26581 agtggcattt agatctatga cgtatctggc aaaccatgcc cgatgcgacg ctgtcgcgtc
    26641 ttatcgtgcc tacaaatagt ccgaaccgta ggccggataa ggcgtttacg ccgcatccgg
    26701 caagtggtta aacccgctca aacaccgtcg caataccctg acccagaccg atacacatcg
    26761 tcgccagacc aaactgaacg tctttgcgtt ccatcagatt cagcagcgtg gtgctgatac
    26821 gcgcaccgga acagcccagc ggatgaccca gcgcgatcgc accaccattg aggttgatct
    26881 tctcgtcaat ctgctccatt agcccgagat ctttaatgca cggcaggatc tgcgcggcaa
    26941 aggcttcgtt catctcaaac acgccgatat cgctggcaga aagccccgct tttttcagcg
    27001 ccagcttcga ggccggaacc gggccgtaac ccataatcga tgggtcacaa ccaacgaccg
    27061 ccatcgaacg cacacgagcg cgcggcttaa gacctaattc atgggcgcgg ctttcactca
    27121 tcaccagcat ggcagctgcg ccatcggaaa gtgcagaaga tgtgcccgcc gttaccgtac
    27181 cgtttactgg atcaaacgcc ggacgcagcg tggcgagggc ttccacggtg gtttccgggc
    27241 gaatcacttc gtcgtaatta aactgcttca ggacgccgtc ggcatcgtga ccaccagttg
    27301 ggatgatttc atttttaaat gcggccgact gcgtggcggc ccaggcgcgg gcgtgtgacc
    27361 gcgcggcaaa ggcatcctgc atttcacggc tgataccgtg catacgcgcc agcatttctg
    27421 ctgttaagcc catcatgccc gccgctttgg cgacattgcg gctcaggccg gggtgaaaat
    27481 cgacgccgtg actcatcggc acatggccca tatgctccac gccgccaacc agacatgcct
    27541 gcgcatcgcc ggtcatgatc attcgtgctg cgtcatgcag tgcctgcatg gatgaaccac
    27601 acaagcgatt gacggtaacc gccgggacag agtgcggcac ttctgccagc agcgccgcgt
    27661 tacgggcgat gttaaaaccc tgctccagcg tctgctgcac acaaccccag taaatatcgt
    27721 cgagggccgc cgcttccagc gccgggttac gcgccagcag gctacgcatt aaatgagcgg
    27781 agagatcttc cgcacgcacg ttacgaaaag caccgccctt cgaacggccc atcggggtgc
    27841 gaattgcatc gacaatgaca acctgttcca ttgtgactcc ttaagccgtt ttcaggtcgc
    27901 caaccggacg ggctggctca accgggggat agtacggttc gttatgacgt gctttattac
    27961 gcagaccttc cggcacttcg tacagcgggc cgaggtgctg atattgctgt gccatatcga
    28021 ggtattttgc gctaccgagg gtgtccagcc agcggaacgc gccgccgtgg aacggaggga
    28081 agcccaggcc gtagaccagc gccatatccg cttccgccgg agtggcgata atgccttcct
    28141 ccagacagcg caccacttcg ttgaccatcg ggatcatcat gcgggcgata atctcttctt
    28201 ctctgaaatc gcgcttcggc tggctcactt ctgccagcag gtcttcaacg gcggcgtctt
    28261 cttctttctt cggcttacct ttgctgtctt ctttataacg ccagaaaccg aggccgttct
    28321 tctgaccaaa gcggttggca tcaaacagcg cgtcgatggc atcgcggtaa tctttctgca
    28381 tccgctgcgg gaagcctgct gccatgacag cctgagcgtg atgcgcggta tcaatgccca
    28441 cgacatccag cagatatgcc ggacccatag gccagccaaa ctgtttttcc atcactttgt
    28501 cgatcttgcg gaaatccgcg ccgtcgcgca gcagctggct gaaaccggcg aaatacggga
    28561 acagcacgcg gttaacaaag aagccggggc agtcgttaac cacaatcggc gtcttgccca
    28621 tcttgctcgc ccaggcgaca actttcgcga tggtttcgtc ggagcttttc tcgccgcgaa
    28681 taatttctac caacggcatc cggtgtaccg ggttaaaaaa gtgcattccg cagaagtttt
    28741 ccgggcgttc cagcgcgttg gccagttcgc tgataggaat ggttgaagtg ttagacgcca
    28801 gcacggtatc cgggcgtact ttttgttcgg tttctgccag tacggctttt ttcactttcg
    28861 ggttttcgac aaccgcttct accacaacat ccacgcggtc aaacccggcg tagtcgagcg
    28921 ttgggtggat tgtggagatc acgccagcca gtttcagacc atcgatcttg ccgcgctcaa
    28981 gctgcttgtt cagcagtttc gcggcttcgg tcatgccgag ggttaacgac ttgtcgttga
    29041 tatctttcat gacaaccggc acgcctttcc acgcagactg gtaagcgatg ccgccgccca
    29101 taatgcctgc acccagcacc gcggcctgtt tcggggtttc aacgtctttg gtgagtttct
    29161 tcgctttgcc ttttacatat tgatcgttaa ggaaaatgcc gaccagtgcg cgggcttcgt
    29221 tggtatgcgc cagcgggaca aaacttttgt tttccaggtt taaggcttct tcacgaccaa
    29281 aacgggccgc agcttcaatg gtttttactg cggtgatggg ggccggataa tgtttccccg
    29341 ctgtttgtgc gaccatccct ttagcgatgg tgaagctcat ggtggcttca atcttgctca
    29401 gttttagtgg ttccagcttc ggctgacgtt ttgctttcca gtcgaggtcg ccgttaatgg
    29461 cctggcgtaa aaccgccttt gcgccttcaa ccagtttttc tgctttgact acgccatcca
    29521 ccagaccgat tttcagcgcc tgatccgcgc cgacatcttt accggcggca atgatttcca
    29581 gcgcactgtc agcgcccagc atacgtggca tacgtacaga accgccaaag cccggcatga
    29641 tacccagttt ggtttccggc agaccgatgc gcagatccgg cgtcgccaga cgataatcgg
    29701 tcgccagcac gcattcgcag ccaccgccca gcgcatagcc gttgacggca gcaatggtcg
    29761 gcaccggcag atcttccagg cgattaaaca cgctattggc aaattgcagc cactgactta
    29821 actgttcttc aggaacgagg aacagggaca aaaattcggt gatatcagca ccgacgataa
    29881 aggctgcttt gttcgaacgc agcagcagcc cttttagatc tgattgctgt tccagcacgc
    29941 cgatggcctc gccgaggctg gcgacggtcg cagtgtcgag tttattaact gaacctgggg
    30001 catcaaatac cagttcggca atgccatctt ccagccagtc aaggtacagg gtgtcgcctt
    30061 tgtaaagcat gtcagtctcc tgaatccgca aggtgatctg gtcgtaccag atgagtcgaa
    30121 gtgtgtattt tgtgttaaaa atatgcaaac aaaagattaa agaaatgccg atctgatcac
    30181 gctcggcaga aatcacgctc tggatgaacg atgtgctaag atgcggagac ttaaggtcaa
    30241 aaaaacagaa gggtaaaaaa tggaatcact ggcctcgctc tataaaaatc atatagctac
    30301 cttacaagaa cggactcgcg atgcgctggc gcgcttcaag ctggatgcgt tacttattca
    30361 ctccggcgag ctgttcaatg tttttctcga cgatcatccc tatccgttta aagtgaaccc
    30421 gcaattcaaa gcgtgggtgc cggtaactca ggtgccaaac tgctggttgc tggtggatgg
    30481 cgtgaacaag ccgaaactgt ggttttatct gccggttgat tactggcaca acgtcgaacc
    30541 gctgccgacc tccttctgga ctgaagatgt ggaagtgatc gcgctgccga aagccgatgg
    30601 cattggtagc ctgctgcctg ctgcgcgcgg caatatcggt tatatcggtc cggtgccgga
    30661 gcgtgcgctg caactgggta ttgaggccag caacatcaac ccgaaagggg ttatcgacta
    30721 cctgcattac tatcgctcct tcaaaaccga gtacgaactg gcctgtatgc gtgaagcgca
    30781 gaaaatggcg gtcaacggtc atcgtgcggc agaagaagcg ttccgttctg gcatgagcga
    30841 gttcgatatc aacattgcct atctgaccgc gaccggtcat cgtgataccg acgtacctta
    30901 cagcaacatt gtggcgctta acgaacacgc tgcggtgctg cattacacca aactggatca
    30961 tcaggcgccg gaagagatgc gcagcttcct gctggatgcc ggggccgaat ataacggcta
    31021 tgcggctgac ctgactcgta cctggtcggc aaaaagcgac aacgactacg cacagctggt
    31081 gaaagacgta aatgatgaac aactggcgct gatcgccacc atgaaagcag gcgttagcta
    31141 tgtggattac cacatccagt tccatcagcg catcgccaaa ctgctgcgta aacatcaaat
    31201 catcaccgat atgagtgaag aagcgatggt cgaaaacgat ctcaccggac cgtttatgcc
    31261 gcacggtatc ggtcatccgc tgggcctgca ggtgcatgac gtagccggtt ttatgcagga
    31321 tgatagcggt acacacctcg cggcaccggc aaaatatccg tacctgcgct gcacccgtat
    31381 tctccagccg ggcatggtgt taaccatcga accgggtatc tacttcattg aatcgctact
    31441 ggcaccgtgg cgtgaagggc agttcagcaa gcacttcaac tggcagaaaa ttgaagcact
    31501 gaaaccgttc ggcggcattc gtatcgaaga caacgtggtg atccacgaaa acaacgtgga
    31561 aaacatgacc cgggatctga aactggcgtg atggaaagct ggttaattcc tgcggcaccg
    31621 gtcacggtcg ttgaagagat caaaaagagc cgcttcataa cactgttggc gcataccgat
    31681 ggcgttgagg cggcgaaagc gtttgttgaa tcggtgcggg cagaacaccc cgatgcccgc
    31741 caccattgcg tggcgtgggt ggcgggtgcg ccggatgatt ctcaacagct gggtttctct
    31801 gacgacgggg agccggcagg aacggcaggt aaaccgatgc tcgcccagct aatgggcagc
    31861 ggcgtcgggg aaattaccgc tgtggtagtg cgctactacg gtggcatatt gcttggcacc
    31921 ggtgggttag tgaaagcgta tggcggcggc gtgaatcagg cgctgcgcca gctaacgacc
    31981 caacgcaaga cgccattaac cgaatatact ttgcaatgtg aatatagtca gttaaccggc
    32041 attgaagcgt tgctggggca gtgtgacggc aaaattatca acagtgatta tcaggcattc
    32101 gttctgctgc gggtggcgct tccggcggcg aaagtggctg aattttccgc aaagctggcg
    32161 gattttagcc gtggttcatt gcaattgtta gcgattgaag aataatcccc acttcgtttt
    32221 gcagaactaa ggaagcggca gagatgcatt ttcgcgccat tacccgaatc gttggactac
    32281 tggtcatctt attttcaggg accatgatta tccctgggct ggtagcactc atctaccggg
    32341 atggagcggg ccgcgctttt acccagacct tttttgtcgc cctcgccatt ggttctatgc
    32401 tgtggtggcc gaaccgtaaa gagaagggcg aactgaaatc ccgtgagggg tttctgatag
    32461 tggtgctgtt ctggaccgtg ctgggtagtg tcggtgcgct cccttttatc ttctcggaaa
    32521 gcccgaacct cacgattacc gatgcgtttt ttgaatcttt ctctggcctg accaccaccg
    32581 gggccactac gctggtgggg ctggattcgc tccctcacgc catcctcttt tatcgccaga
    32641 tgctgcaatg gtttggcggg atggggatca tcgtgttagc ggttgcgata ctgcctatcc
    32701 tcggcgtggg tgggatgcag ctctatcgcg cagaaatgcc cggcccgctg aaagataaca
    32761 aaatgcgccc gcgaattgcg gaaacggcga aaaccctgtg gttgatttat gtcttgctga
    32821 ccgtcgcctg tgcgctggcg ttgtggtttg ccggaatgga tgcctttgat gccatcggcc
    32881 atagctttgc gactatcgct attggcggct tctcgacaca tgatgccagt atcggttatt
    32941 tcgacagccc gactattaac actatcattg ctatcttcct gctgatctcc ggctgtaact
    33001 acggtctgca cttttcactg ttaagtgggc gtagtctgaa ggtttattgg cgcgatccgg
    33061 aatttcgcat gtttatcggc gtacagttta cgctggtggt tatttgtacc ctcgtactgt
    33121 ggtttcacaa tgtctacagt tcggcgctga tgacaattaa ccaggcgttt ttccaggtgg
    33181 tgtcgatggc gacaaccgcc gggtttacga ctgacagcat tgcccgctgg ccgctctttt
    33241 tgccggtact gcttttatgt tcagctttta tcggcggttg tgccgggtca acgggcggtg
    33301 gcctgaaagt gatccgcatc ctgctgctgt ttaagcaggg gaaccgtgag ctgaaacgac
    33361 tggtgcatcc gaacgccgtc tatagcatta agctggggaa tcgcgcactg ccggaacgta
    33421 tcctcgaagc cgtttgggga tttttctccg cctatgcatt ggtgtttatt gtcagtatgc
    33481 tggcgattat cgccacgggc gtggatgact tttctgcttt tgcctcggtt gttgcgacat
    33541 tgaataacct ggggccgggg cttggcgtgg ttgctgataa ctttaccagt atgaacccgg
    33601 tggctaaatg gatcctgatt gccaacatgc tgtttggtcg tctcgaggtc tttacattgc
    33661 tggtgctctt taccccgact ttctggcgtg aatgatggag taatacgtga aaacattaat
    33721 tcttttctca acaagggacg gacaaacgcg cgagattgcc tcctacttgg cttcggaact
    33781 gaaagaactg gggatccagg cggatgtcgc caatgtgcac cgcattgaag aaccacagtg
    33841 ggaaaactac gaccgtgtgg tcattggtgc ttctattcgc tatggtcact accactctgc
    33901 gttccaggaa tttgtcaaaa agcatgcgac gcggctgaat tcgatgccga gcgcctttta
    33961 ctccgtaaac ctggtggcgc gtaaaccgga gaagcgcact ccacagacca acagctacgc
    34021 gcggaagttt ctgatgaact cgcaatggcg tcccgatcgc tgcgcggtca ttgccggggc
    34081 gctgcgttac ccacgttatc gctggtacga ccgttttatg atcaagctaa ttatgaagat
    34141 gtcaggcggt gaaacggata cgcgcaaaga agttgtctat accgattggg agcaggtggc
    34201 gaatttcgcc cgagaaatcg cccatttaac cgacaaaccg acgctgaaat aagcataaag
    34261 aataaaaaat gcgcggtcgg aaaattattt taaatttcct cttgtcaggc cggaataact
    34321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    34381 ttctcctgag aattccg
//
LOCUS       DAJOWB010000039        34347 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJOWB010000039 DAJOWB010000000
VERSION     DAJOWB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.039"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..442)
                     /locus_tag="OHO85_002069"
     CDS             complement(<1..442)
                     /locus_tag="OHO85_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCQ2671444.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVL"
     gene            complement(602..814)
                     /gene="rpmE"
                     /locus_tag="OHO85_002070"
     CDS             complement(602..814)
                     /gene="rpmE"
                     /locus_tag="OHO85_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCQ2671445.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            1017..3215
                     /gene="priA"
                     /locus_tag="OHO85_002071"
     CDS             1017..3215
                     /gene="priA"
                     /locus_tag="OHO85_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCQ2671446.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDSEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQVGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3371..4396
                     /gene="cytR"
                     /locus_tag="OHO85_002072"
     CDS             3371..4396
                     /gene="cytR"
                     /locus_tag="OHO85_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCQ2671447.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4488..5447
                     /gene="ftsN"
                     /locus_tag="OHO85_002073"
     CDS             4488..5447
                     /gene="ftsN"
                     /locus_tag="OHO85_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCQ2671448.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVTRAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5540..6070
                     /gene="hslV"
                     /locus_tag="OHO85_002074"
     CDS             5540..6070
                     /gene="hslV"
                     /locus_tag="OHO85_002074"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCQ2671449.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            6080..7411
                     /gene="hslU"
                     /locus_tag="OHO85_002075"
     CDS             6080..7411
                     /gene="hslU"
                     /locus_tag="OHO85_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCQ2671450.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7478..8404
                     /gene="menA"
                     /locus_tag="OHO85_002076"
     CDS             7478..8404
                     /gene="menA"
                     /locus_tag="OHO85_002076"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCQ2671451.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8497..8982
                     /gene="rraA"
                     /locus_tag="OHO85_002077"
     CDS             8497..8982
                     /gene="rraA"
                     /locus_tag="OHO85_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCQ2671452.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(9067..9312)
                     /gene="zapB"
                     /locus_tag="OHO85_002078"
     CDS             complement(9067..9312)
                     /gene="zapB"
                     /locus_tag="OHO85_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCQ2671453.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9738..10583
                     /gene="glpF"
                     /locus_tag="OHO85_002079"
     CDS             9738..10583
                     /gene="glpF"
                     /locus_tag="OHO85_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCQ2671454.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10606..12114
                     /gene="glpK"
                     /locus_tag="OHO85_002080"
     CDS             10606..12114
                     /gene="glpK"
                     /locus_tag="OHO85_002080"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCQ2671455.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            12249..13259
                     /gene="glpX"
                     /locus_tag="OHO85_002081"
     CDS             12249..13259
                     /gene="glpX"
                     /locus_tag="OHO85_002081"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCQ2671456.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13356..14102
                     /gene="fpr"
                     /locus_tag="OHO85_002082"
     CDS             13356..14102
                     /gene="fpr"
                     /locus_tag="OHO85_002082"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCQ2671457.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(14107..14535)
                     /gene="uspD"
                     /locus_tag="OHO85_002083"
     CDS             complement(14107..14535)
                     /gene="uspD"
                     /locus_tag="OHO85_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCQ2671458.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14562..14861)
                     /locus_tag="OHO85_002084"
     CDS             complement(14562..14861)
                     /locus_tag="OHO85_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCQ2671459.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(15073..15513)
                     /locus_tag="OHO85_002085"
     CDS             complement(15073..15513)
                     /locus_tag="OHO85_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCQ2671460.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15614..16213
                     /locus_tag="OHO85_002086"
     CDS             15614..16213
                     /locus_tag="OHO85_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCQ2671461.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16321..17088
                     /gene="tpiA"
                     /locus_tag="OHO85_002087"
     CDS             16321..17088
                     /gene="tpiA"
                     /locus_tag="OHO85_002087"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCQ2671462.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(17143..17898)
                     /gene="cdh"
                     /locus_tag="OHO85_002088"
     CDS             complement(17143..17898)
                     /gene="cdh"
                     /locus_tag="OHO85_002088"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCQ2671463.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVRKQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(18005..18994)
                     /gene="sbp"
                     /locus_tag="OHO85_002089"
     CDS             complement(18005..18994)
                     /gene="sbp"
                     /locus_tag="OHO85_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCQ2671464.1"
                     /translation="MNKWGVGLTFLLAATNVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGENVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19313..20275)
                     /gene="pfkA"
                     /locus_tag="OHO85_002090"
     CDS             complement(19313..20275)
                     /gene="pfkA"
                     /locus_tag="OHO85_002090"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCQ2671465.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20456..21358)
                     /gene="fieF"
                     /locus_tag="OHO85_002091"
     CDS             complement(20456..21358)
                     /gene="fieF"
                     /locus_tag="OHO85_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCQ2671466.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21507..22007)
                     /gene="cpxP"
                     /locus_tag="OHO85_002092"
     CDS             complement(21507..22007)
                     /gene="cpxP"
                     /locus_tag="OHO85_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCQ2671467.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            22157..22855
                     /gene="cpxR"
                     /locus_tag="OHO85_002093"
     CDS             22157..22855
                     /gene="cpxR"
                     /locus_tag="OHO85_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCQ2671468.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22852..24225
                     /gene="cpxA"
                     /locus_tag="OHO85_002094"
     CDS             22852..24225
                     /gene="cpxA"
                     /locus_tag="OHO85_002094"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCQ2671469.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24331..25005)
                     /gene="yiiM"
                     /locus_tag="OHO85_002095"
     CDS             complement(24331..25005)
                     /gene="yiiM"
                     /locus_tag="OHO85_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCQ2671470.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(25154..26137)
                     /gene="kdgT"
                     /locus_tag="OHO85_002096"
     CDS             complement(25154..26137)
                     /gene="kdgT"
                     /locus_tag="OHO85_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCQ2671471.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPIITSIWSRKVKARAAKIEILGTVK"
     gene            complement(26397..27017)
                     /gene="sodA"
                     /locus_tag="OHO85_002097"
     CDS             complement(26397..27017)
                     /gene="sodA"
                     /locus_tag="OHO85_002097"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCQ2671472.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27302..28336
                     /gene="rhaT"
                     /locus_tag="OHO85_002098"
     CDS             27302..28336
                     /gene="rhaT"
                     /locus_tag="OHO85_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCQ2671473.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLITHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28333..29199)
                     /gene="rhaR"
                     /locus_tag="OHO85_002099"
     CDS             complement(28333..29199)
                     /gene="rhaR"
                     /locus_tag="OHO85_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCQ2671474.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(29255..30091)
                     /gene="rhaS"
                     /locus_tag="OHO85_002100"
     CDS             complement(29255..30091)
                     /gene="rhaS"
                     /locus_tag="OHO85_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCQ2671475.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVAEQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30379..31848
                     /gene="rhaB"
                     /locus_tag="OHO85_002101"
     CDS             30379..31848
                     /gene="rhaB"
                     /locus_tag="OHO85_002101"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCQ2671476.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLKERKINDLPALIVATQSLPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVARIHSTRKTKELCA"
     gene            31845..33104
                     /gene="rhaA"
                     /locus_tag="OHO85_002102"
     CDS             31845..33104
                     /gene="rhaA"
                     /locus_tag="OHO85_002102"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCQ2671477.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            33187..34011
                     /gene="rhaD"
                     /locus_tag="OHO85_002103"
     CDS             33187..34011
                     /gene="rhaD"
                     /locus_tag="OHO85_002103"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCQ2671478.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWSFHGVFGSG
                     PTLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            34021..34335
                     /gene="rhaM"
                     /locus_tag="OHO85_002104"
     CDS             34021..34335
                     /gene="rhaM"
                     /locus_tag="OHO85_002104"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCQ2671479.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         8387 a         8965 c         8938 g         8057 t
ORIGIN      
        1 tcaggacgcc gccgcgcacc agccacagtg actcgctgcg gctgtaaccg tcctcaccgg
       61 gaaacaggtg ctcaaaatac aggcttctgc cgccgttatc actgagtatc agtgtgttat
      121 cgcgcagctg taagcggata tccgcaggca ttttccagcc ggggccgagg ctccccaccg
      181 gcgcgggcgt ttttgtccgg taactgctgt aggtgcggga gagaatgaac ggcagcgggc
      241 cgggcagggc gatgtcggtt tcaccgggaa ggacctttgc accgagcagc ggattgaccg
      301 gatggccgga cgtcaccccg ccggggcaca ccgaacaggc cacgccggtg ggggcaccga
      361 tgcgcacccc ggctgaaccc tggacaatgc taccgccata ctgcgtcatg tcgccctgac
      421 gcgccgccgg ttttccgctc atcgtaaaat ccttttgaaa taaataagaa ccagaagcct
      481 agcgccagtc aacaacagga aatgataatg agttaacacc ccggaatttt tcagaattta
      541 taaaaggcaa attacaggca caaaaaaagc gccgtgcggc gcttttttcg gaaatccggt
      601 cttatttgct gcccgggatg ttgaaacgct tgttgaagcg gtcaacacgg ccaccggtag
      661 caacatcacg ctgtttgcca gtgaagaacg ggtggcactt gctgcacacg tcgaggttca
      721 ggtcatgacc aacggtggag cggattttca ttacgttacc gcaagagcag ctagcagtaa
      781 tttcttcgta tttcgggtga atatcttttt tcatgggaaa acctcggttt aaggccgcgt
      841 cgctcttcca gccctaacgc cagacaccac gcgatgttaa aagtatagct tcaatacgat
      901 catttcgtac gaaggcgcga aatcatacag aaattaacca gcgtatgcaa actgatccgc
      961 actcttctac ggcaatgtgt atactaaccc accgaatttc aagtcaggat gatgctatgc
     1021 ccgttgccca cgttgccttg cccgttccgc ttcctcgtac ctttgactat ctgctgccag
     1081 aaggcatgac ggttaaagct gggtgtcgcg tgcgcgtgcc gtttggcaaa cagcaggagc
     1141 gcatcgggat tgtggtatca gttagcgatg ccagcgaact gccgctcaat gagctaaaag
     1201 cggtagtcga agtgctggat agtgagccgg tgtttactca ctccgtctgg cgattgctgc
     1261 tatgggcggc agattactat catcatccga ttggcgatgt gctgtttcat gccttgccga
     1321 ttttactacg ccaggggcgg cctgcggcga acgcgccgat gtggtactgg tttgccactg
     1381 aacaaggtca ggcggtggat ctgaacagcc tgaaacgctc acccaagcaa caacaggcgc
     1441 tggcagcgtt acggcaaggc aaaatctggc gcgaccaggt tgccacgctc gaatttaatg
     1501 atgccgcgtt gcaggcgctg cgcaaaaaag gtctgtgtga tttagcaagt gaaacaccag
     1561 agtttagcga ctggcgaacg aactatgccg tttctggtga gcggttgcga ttgaataccg
     1621 aacaggccac cgccgttggc gcaattcata gtgcggcgga taccttctct gcctggctgc
     1681 tggcgggcgt taccggttcc ggtaaaacgg aggtttatct cagtgtactg gaaaacgtgc
     1741 tcgctcaggg caaacaggcg ctggtgatgg tgccggaaat cggcctgaca ccgcaaacta
     1801 tcgcccgttt tcgtgaacgt tttaatgccc ccgtagaagt tctgcattcc ggcttgaacg
     1861 acagcgagcg tctttcggcg tggttgaaag cgaaaaatgg tgaggcggcg attgtgatcg
     1921 gcacccgctc cgcgctgttt acgccgttta aaaatctcgg tgtgattgtc attgatgaag
     1981 agcacgacag ttcctacaag cagcaggaag gctggcgcta tcatgcccgc gacctggcgg
     2041 tttatcgtgc gcacagcgag caaatcccga ttattcttgg ctccgcaaca cccgcactgg
     2101 aaacgttatg caacgtccag cagaaaaaat accgcctgct gcgcctgacg cgtcgggcgg
     2161 ggaatgcgcg tccggcaatt caacacgtgc tggatttaaa aggtcagaag gtgcaggtgg
     2221 ggctggctcc ggcgttaatc acccgtatgc gccagcattt acaggccgac aaccaggtta
     2281 tcctctttct taaccgccgt ggctttgcgc ctgccttgct gtgccacgac tgcggctgga
     2341 ttgccgaatg tccacgttgc gatcactact acacgctgca tcaggcgcaa cagcatttgc
     2401 gctgtcacca ctgtgacagc caacgcccgg tgccgcgcca gtgcccttcc tgcggttcca
     2461 cgcacctagt acccgtgggg ctgggcaccg aacagcttga acagacgctt gcgccgatgt
     2521 tccccggcgt gcccatttca cgtatcgacc gcgataccac cagccgcaaa ggggcgctgg
     2581 aacagcaact ggcggaagtg catcgcggcg gcgcgaggat cttgattggc acgcaaatgc
     2641 tggcgaaagg tcaccacttt ccggatgtga cgctggtggc gttactggat gtggacggcg
     2701 cgctgttttc tgccgatttt cgctcggcag aacgtttcgc ccagctttac acccaggtcg
     2761 ccggtcgtgc cgggcgtgcg ggtaaacagg gcgaagtggt gctgcaaacg caccatccgg
     2821 aacatcctct gttgcaaacg ttgctctata aaggctacga cgcctttgcc gaacaggcgc
     2881 tggctgagcg acgcatgatg cagctgccgc cgtggaccag ccatgtgatt gtgcgtgcgg
     2941 aagatcataa caatcagcat gcgccgctgt tcctgcaaca actgcgtaat ctgatccttt
     3001 ccagcccgct ggcagacgac aaactgtggg ttctcggccc ggttccggct ctggcaccta
     3061 agcgtggtgg ccgctggcgc tggcagatct tgttgcagca cccttcccgc gtgcgcttgc
     3121 aacacatcat tagtggtacg ctggcgctta ttaatacaat accggattcc cgcaaggtga
     3181 aatgggtgtt ggatgtcgat cccatcgaag gctaatgcgc aaacgttgcg aggcggatcg
     3241 aaaaattcaa tattcatcac acttttcatg aaaattctgt aaccgttttc acgcgctatc
     3301 tgctaaaaat gttgccgatg tgaagtaaac atggatgtag tacgcctgac gtgccaggcg
     3361 aggagtgagt gtgaaagcga agaagcagga aactgccgcg accatgaaag acgttgccct
     3421 caaggcaaaa gtctctacag cgactgtctc ccgagcatta atgaatcccg ataaagtctc
     3481 ccaggccacc cgtaatcggg ttgaaaaagc ggcccgggaa gtgggttatt taccgcaacc
     3541 tatggggcgc aacgtcaagc gtaatgaatc ccgcaccatt ctggtgattg tcccggatat
     3601 ctgcgatccc ttctttagcg aaattattcg cggtatcgaa gttacggcgg caaatcacgg
     3661 atatctggtg ctgattggcg actgtgcgca tcaaaatcag caggaaaaaa cctttatcga
     3721 tttgatcatc accaagcaaa ttgatggcat gttgctgctg ggctcaaggc tgccgtttga
     3781 tgccagcatt gaggaacagc gtaatctgcc gccgatggtg atggcgaacg aatttgcacc
     3841 ggaactggag ctgcctaccg ttcatatcga caatctgacc gccgcatttg atgcagtaaa
     3901 ttatttatat gagcaagggc ataaacggat tggctgtata gccggtcccg aagagatgcc
     3961 actgtgtcac taccgcctgc aaggctatgt tcaggcgctg cgtcgctgcg gcattatggt
     4021 tgatccgcaa tacatcgccc gtggcgactt caccttcgaa gccggaagca aagcgatgca
     4081 gcagctgctt gatcttccac aaccgcctac tgctgtcttc tgccatagcg atgtgatggc
     4141 gctcggcgca ctttctcagg caaaacgcca ggggctgaaa gtcccggaag acctttccat
     4201 aatcggtttt gataacatcg acctgacgca attttgtgat ccgccgctga caaccatcgc
     4261 gcagccgcgt tacgaaatcg gtcgggaagc tatgctgtta ttgcttgatc aaatgcaggg
     4321 gcaacacgtt ggcagtggct ctcgtttaat ggactgcgaa cttatcatcc ggggatcaac
     4381 acgcgcgtta ccttaaagta aacctttaag actgccgtga ctggtcaaag gcccgccgct
     4441 taagtaacat ggcgggctga cgaacgaata aatacagcga aacgatagtg gcacaacgag
     4501 attatgtacg ccgcagccaa ccggcacctt cgcggcgaaa aaagagcacc tcacggaaaa
     4561 agcaacgaaa tctgcctgcg gtttctcccg ctatggtcgc tattgctgcc gccgttcttg
     4621 tgacctttat cggtggtctg tacttcatta cgcatcacaa gaaagaagag tccgagacgc
     4681 tgcaaagcca gaaagtgacc ggaaacggac taccaccaaa accagaagaa cgctggcgct
     4741 acatcaaaga gctggaaagt cgccagccgg gtgtgcgtgc gcccacagaa ccttctgctg
     4801 gtggtgaagt gaaaacgccg gagcaactga caccagaaca acgtcagctt cttgaacaaa
     4861 tgcaggctga tatgcgccag cagccaacgc agctggttga ggttccgtgg aatgaacaga
     4921 cgccggaaca gcgtcagcaa acgctacagc gccaacgtca ggcgcagcaa ttagcggaac
     4981 agcaacgcct ggctcagcag tcgcgtacga ctgaacaaag ctggcagcag cagacgcgta
     5041 cgtctcaagc cgcgccggta caggcgcaac cgcgccagtc taaaccggct tctactcagc
     5101 agccgtacca ggatctgctg caaacccctg cgcacacaac cgcgcagtcg aagccgcagc
     5161 aagctgcgcc ggttactcgt gccgctgacg cgccaaaacc gacggcggag aaaaaagacg
     5221 aacgccgctg gatggtgcag tgcggttcgt tcagaggcgc ggaacaggca gagacggtac
     5281 gtgctcagct ggcgttcgaa ggatttgact cgaaaatcac caccaacaat ggctggaatc
     5341 gtgtggtcat tggcccggtg aaaggcaaag agaacgcaga cagcaccctc aatcggttga
     5401 agatggcggg tcatacaaac tgcattcggc tcgccgccgg gggttgaaac cctcaaaatc
     5461 ccccccatct ataattgcat tatgccccgt acttttgtac ggggtttgta ctctgtattc
     5521 gtaaccaagg ggtcagctcg tgacaactat agtaagcgta cgccgtaacg gccatgtggt
     5581 catcgctggt gatggtcagg ccacgttggg caataccgta atgaaaggca acgtgaaaaa
     5641 ggtccgccgt ctgtacaacg acaaagtcat cgcgggcttt gcgggcggta ctgcggatgc
     5701 ttttacgctg ttcgaactgt ttgaacgtaa actggaaatg catcagggcc atctggttaa
     5761 agccgccgtt gagctggcaa aagactggcg taccgatcgc atgctgcgca aacttgaagc
     5821 actgctggca gtcgcggatg aaactgcatc gcttatcatc accggtaacg gtgacgtggt
     5881 gcagccagaa aacgatctta ttgctatcgg ctccggcggc ccttacgccc aggctgcggc
     5941 gcgcgcgctg ttagaaaaca ctgaacttag cgcccgtgaa attgctgaaa aggcgttgga
     6001 tattgcaggc gacatttgca tctataccaa ccatttccac accatcgaag aattaagcta
     6061 caaagcgtaa ggatctccca tgtctgaaat gaccccacgc gaaatcgtca gcgaactgga
     6121 taagcacatc atcggccagg acaacgccaa gcgttctgtg gcgattgctc tgcgtaaccg
     6181 ctggcgtcgc atgcagctca acgaagagct gcgccatgaa gtgaccccga aaaatatcct
     6241 gatgatcggc ccgaccggtg tcggtaaaac tgaaatcgcc cgtcgtctgg ctaagctggc
     6301 gaatgcgccg ttcatcaaag ttgaagcgac caaattcacc gaagtgggct acgtcggtaa
     6361 ggaagtggat tctattattc gcgatctgac cgatgccgcc gtgaaaatgg tacgcgtcca
     6421 ggctatcgag aaaaaccgtt atcgcgctga agaactggca gaagaacgta ttctcgacgt
     6481 gctgatccca cctgctaaaa acaactgggg acagaccgaa cagcagcagg aaccgtccgc
     6541 tgctcgtcag gcattccgca aaaaactgcg tgaaggccag cttgatgaca aagaaatcga
     6601 gatcgatctt gccgcagcac cgatgggcgt tgaaattatg gctcctccag gcatggaaga
     6661 gatgaccagc cagctgcagt ccatgttcca gaacctgggc ggccagaagc aaaaagcgcg
     6721 taagctgaaa atcaaagacg ccatgaagct gctgattgaa gaagaagcgg cgaaactggt
     6781 gaacccggaa gagctgaagc aagacgctat cgacgctgtt gagcagcacg ggatcgtgtt
     6841 tatcgacgaa atcgacaaaa tctgtaagcg cggcgagtct tccggtccgg atgtttctcg
     6901 tgaaggcgtt cagcgtgacc tgctgccgct ggtagaaggt tgcaccgttt ccaccaaaca
     6961 cgggatggtc aaaactgacc acattctgtt tatcgcttct ggcgcgttcc agattgcgaa
     7021 accgtctgac ctaatcccgg aactgcaagg tcgtctgcca atccgcgttg aactgcaggc
     7081 actgaccacc agcgacttcg agcgtattct gaccgagccg aatgcctcta tcaccgtgca
     7141 gtacaaagca ctgatggcga ctgaaggcgt aaatatcgag tttaccgact ccggtattaa
     7201 acgcatcgcg gaagcggcat ggcaggtgaa cgaatctacc gaaaacatcg gtgctcgtcg
     7261 tttacacact gttctggagc gtttaatgga agagatttcc tacgacgcca gcgatttaag
     7321 cggtcaaaat atcactattg acgcagatta tgtgagcaaa catctggatg cgttggtggc
     7381 agatgaagat ctgagccgtt ttatcctata atcgcgttca atcattttca tcattgtttg
     7441 atggggctga aaggccccat ttttattggc gcgtattatg actgaacaac aaattagccg
     7501 aactcaggcg tggctggaaa gtttacgacc taaaaccctc cccctcgcct ttgctgcaat
     7561 tatcgtcggg acggcgctgg catggtggca aggtcacttc gatccgctgg tcgccctgct
     7621 ggcactaatt accgccgggc tattacagat cctttctaac ctcgccaatg attacggcga
     7681 tgcggtaaaa ggcagcgata aacctgaccg cattgggccg ctacgcggca tgcaaaaagg
     7741 ggtcattacc cagcaagaga tgaaacgggc gctcattatt accgtcgtgc tcatctgtct
     7801 ctccgggctg gcactggttg cagtggcatg ccatacgctg gccgattttg tcggtttcct
     7861 gattcttggc gggttgtcga tcattgccgc tatcacctac accgtgggca atcgtcctta
     7921 tggttatatc ggtctgggtg atatttccgt actggttttc tttggctggt tgagcgtcat
     7981 ggggagctgg tatttacagg ctcatacatt gattccggca ctgatccttc cggcgaccgc
     8041 ctgtggcctg ctggcaacgg cagtactgaa cattaataac ctgcgtgata tcaatagcga
     8101 ccgcgaaaat ggcaaaaaca cgctggtggt gcgcttaggt gaagtgaacg cgcgtcgtta
     8161 tcatgcctgc ctgctgatgg gctctctggt atgtctggcg ctgtttaatc tcttttcgct
     8221 gcatagcctg tggggctggc tgttcctgct ggcggcacca ttactggtga agcaagcccg
     8281 ttatgtgatg cgggaaatgg acccggtggc gatgcgacca atgctggaac gtactgtcaa
     8341 gggagcgtta ctgactaacc tgctgtttgt tttagggata ttcctaagcc agtgggcagc
     8401 ataactgaca aatatcaatt aacaattgat gattttgcca acagcccaca tagcgcgata
     8461 tactgaaaat tctcgcagca actgaatgtt aagcctatga aatacgatac ttccgagctt
     8521 tgtgacatct atcaagaaga tgttaacgtc gtggaaccgc tgttctccaa ctttggcgga
     8581 cgggcgtcgt ttggcggaca aataatcacg gtaaaatgtt tcgaggacaa cgggttgctg
     8641 tacgatctgc tcgaacagaa tggccgtggt cgtgttcttg tcgtcgatgg cggtggttct
     8701 gttcgtcgcg cactggtcga tgctgaactg gcgcgtctgg cagtacaaaa tgaatgggaa
     8761 ggtctggtta tttacggcgc ggtgcgtcag gtagatgacc tggaagagtt ggatatcggc
     8821 atccaggcga tggcggcaat tccggttggt gccgctggcg aaggcattgg cgaaagcgat
     8881 gtccgcgtca attttggcgg tgtcaccttc ttctccggcg accatcttta tgccgacaat
     8941 accgggatta ttctttcaga agatccgctg gatattgaat gataagaaag gcaccgcaag
     9001 gtgccttttt tctgcgttac ctgttggcct acacagtaaa gaaattacgc gaaagatgaa
     9061 gcgtaatcag acctcttcca tgcgacccag cagggcctgc agacgttcct gccagccgtt
     9121 ctgctgttct ttcagatggt tgttctcacg ctccagctct tcgcgctgat gctgggcatt
     9181 ttgaacttcc tgcgacagtg agttgttttt ttctttcagc tcttcgattt ccatctgcaa
     9241 cagagtgatg gtatcaatcg cctgctgtac ttttgcttcc agtttctcaa acacttctaa
     9301 tgacattgtc atacctctcc tgaattgcaa ggcgttgatg gataaaaaat cctcgtcccg
     9361 attaccggtg acgccttaat aaatacgagc gcactttagt tagctccgat tgtatgaagc
     9421 cgcgccatcg ctgtccagcg gcacgccttg cagattacgg tttgccacac ttttcatcct
     9481 tctcctggag acataatcca caccaatcga aaatgttaat aaatttgttg cgcgaatgat
     9541 ctaacaaaca tgcatcatgt acaatcagat ggaataaatg gcgcgataac gctcatttta
     9601 tgacgaagca cacacatttt aagttcgata tttctcgttt ttgctcgtta acgataagtt
     9661 tacagcatgc ctacaagcat cgtggaggtc cgtgactttc acgcatacaa caaacattaa
     9721 ctcttcagga tccgattatg agtcaaacat caaccttgaa aggccagtgc attgctgaat
     9781 tcctcggtac cgggttgttg atttttttcg gtgtgggttg cgttgcagca ctaaaagtcg
     9841 ctggtgcgtc ttttggtcag tgggaaatca gtgtcatttg gggactgggg gtggcaatgg
     9901 ctatctacct gaccgcaggg gtttccggcg cgcatcttaa tcccgctgtt accattgcat
     9961 tgtggctgtt tgcctgtttc gacaagcgca aagttattcc ttttatcgtt tcacaagttg
    10021 ccggcgcttt ctgcgctgcg gctttagttt acgggcttta ctacaattta tttttcgact
    10081 tcgagcagac tcatcacatt gttcgcggca gcgttgaaag tgttgatctg gctggcactt
    10141 tctctactta ccctaatcct catatcaatt ttgtgcaggc tttcgcagtt gagatggtga
    10201 ttaccgctat tctgatgggg ctgatcctgg cgttaacgga cgatggcaac ggtgtaccac
    10261 gcggcccttt ggctcccttg ctgattggtc tactgattgc ggtcattggc gcatctatgg
    10321 gcccattgac gggttttgcc atgaacccag cgcgtgactt tggtccgaaa gtctttgcct
    10381 ggctggcggg ctggggtaat gtcgccttta ccggcggcag agacattcct tacttcctgg
    10441 tgccgctttt tggccctatc gttggcgcga ttgtaggtgc atttgcctac cgcaaactga
    10501 ttggtcgcca tttgccttgc gatatctgtg ttgtggaaga aaaggaaacc acaactcctt
    10561 cagaacaaaa agcttcgctg taatatgact acgggacaat taaacatgac tgaaaaaaaa
    10621 tatatcgttg cgctcgacca gggcaccacc agctcccgcg cggtcgtaat ggatcacgat
    10681 gccaatatca ttagcgtgtc gcagcgcgaa tttgagcaaa tctacccaaa accaggttgg
    10741 gtagaacacg acccaatgga aatctgggcc acccaaagct ccacgctggt agaagtgctg
    10801 gcgaaagccg atatcagttc cgatcaaatt gcagctatcg gtattacgaa ccagcgtgaa
    10861 accactattg tctgggaaaa agaaaccggc aagcctatct ataacgccat tgtctggcag
    10921 tgccgtcgta ccgcagaaat ctgcgagcat ttaaaacgtg acggtttaga agattatatc
    10981 cgcagcaata ccggtctggt gattgacccg tacttttctg gcaccaaagt gaagtggatc
    11041 ctcgaccatg tggaaggctc tcgcgagcgt gcacgtcgtg gtgaattgct gtttggtacg
    11101 gttgatacgt ggcttatctg gaaaatgact cagggccgtg tccatgtgac cgattacacc
    11161 aacgcctctc gtaccatgtt gttcaacatc cataccctgg actgggacga caaaatgctg
    11221 gaagtgctgg atattccgcg cgagatgctg ccagaagtgc gtcgttcttc cgaagtatac
    11281 ggtcagacta acattggcgg caaaggcggc acgcgtattc caatctccgg gatcgccggt
    11341 gaccagcagg ccgcgctgtt tggtcagttg tgcgtgaaag aagggatggc gaagaacacc
    11401 tatggcactg gctgctttat gctgatgaac actggcgaga aagcggtgaa atcagaaaac
    11461 ggcctgctga ccaccatcgc ctgcggcccg actggcgaag tgaactatgc gttggaaggt
    11521 gcggtgttta tggcaggcgc atccattcag tggctgcgcg atgagatgaa gttgattaac
    11581 gacgcctacg attccgaata tttcgccacc aaagtgcaaa acaccaatgg tgtgtatgtg
    11641 gttccggcat ttaccgggct gggtgcgccg tactgggacc cgtatgcgcg cggggcgatt
    11701 ttcggtctga ctcgtggggt gaacgctaac cacattatac gcgcgacgct ggagtctatt
    11761 gcttatcaga cgcgtgacgt gctggaagcg atgcaggccg actctggtat ccgtctgcac
    11821 gccctgcgcg tggatggtgg cgcagtagca aacaatttcc tgatgcagtt ccagtccgat
    11881 attctcggca cccgcgttga gcgcccggaa gtgcgcgaag tcaccgcatt gggtgcggcc
    11941 tatctcgcag gcctggcggt tggcttctgg cagaacctcg acgagctgca agagaaagcg
    12001 gtgattgagc gcgagttccg tccaggcatc gaaaccactg agcgtaatta ccgttacgca
    12061 ggctggaaaa aagcggttaa acgcgcgatg gcgtgggaag aacacgacga ataatgtaaa
    12121 tgccgaatga agcgtttatg ccgcatccgg tagtcccgaa acgtgcgggg gcaaccccgc
    12181 acacatcaat aatccctccc ttcccctgtg ctacacttcg cgccattcct tactgcttag
    12241 agtttgctat gagacgagaa cttgccatcg aattttcccg cgtcaccgaa tcagcggcgc
    12301 tggctggcta caaatggtta ggacgcggcg ataaaaacac cgcggacggc gcggcggtaa
    12361 acgccatgcg tattatgctc aaccaggtca acattgacgg caccatcgtc attggtgaag
    12421 gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa agtcggtact ggtcgcggcg
    12481 acgcggtaga tattgctgtt gatccgattg aaggcacgcg catgacggcg atgggccagg
    12541 ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg cttcctcaat gcgccggata
    12601 tgtatatgga gaagctgatt gtcgggccgg gagccaaagg caccattgat ctgaacctgc
    12661 cgctggcgga taacctgcgc aatgtagcgg cggcgctcgg caaaccgttg agcgaactga
    12721 cggtaacgat tctggctaaa ccacgccacg atgccgttat cgctgaaatg cagcaactcg
    12781 gcgtacgcgt atttgctatt ccggacggcg acgttgcggc ctcaattctc acctgtatgc
    12841 cagacagcga agttgacgtg ctgtacggta ttggtggcgc gccggaaggc gtggtttctg
    12901 cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg tctgctggcg cgtcatgacg
    12961 tcaaaggcga caacgaagag aatcgtcgca ttggcgagca ggagctggca cgctgcaaag
    13021 cgatgggcat cgaagccggt aaagtattgc gcctgggcga tatggcgcgc agcgataacg
    13081 tcatcttctc tgccaccggt attaccaaag gcgatctgct ggaaggcatt agccgcaaag
    13141 gcaatatcgc gactaccgaa acgctgctga tccgcggcaa gtcacgcacc attcgccgca
    13201 ttcagtccat ccactatctg gatcgcaaag acccggaaat gcaggtgcac atcctctgat
    13261 tgatttgatc gattgagcct tccagtcctt cgggactgga atttttttgt tcggagaacg
    13321 aagataaggc aagttaatca aaacaggaga aaaacatggc tgattgggta acaggcaaag
    13381 tcactaaagt gcagaactgg accgacgccc tgtttagtct caccgttcac gcccccgtgc
    13441 ttccgtttac cgccgggcaa ttcaccaagc ttggccttga aatcgacggc gaacgcgtcc
    13501 agcgcgccta ctcctatgtt aactcgcccg ataatcccga tctggagttt tacctggtca
    13561 ccgtccccga tggcaaatta agcccacgat tggcggcact gaaaccaggc gatgaagtgc
    13621 aggtggttag cgaagcagct ggcttctttg ttctggatga agtaccggac tgcgaaacgc
    13681 tatggatgct ggcaaccggt acagcgattg gcccttattt atcgattctg caactaggca
    13741 aagatttaga tcgcttcaaa aatctggtcc tggttcacgc cgcacgttat gccgccgact
    13801 taagctattt gccactgatg caagaactgg aaaaacgcta cgaaggaaaa ctgcgcattc
    13861 agacggtggt cagtcgggaa acggcagcgg ggtcgctcac cgggcggata ccggcgttaa
    13921 ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat gaataaagaa accagccatg
    13981 tgatgctgtg cggcaatcca cagatggtgc gcgatacaca acagttgctg aaagagaccc
    14041 ggcagatgac gaaacattta cgtcgccgac cgggccatat gacagcggag cattactggt
    14101 aagcggttac ttatcgataa acggcacgat gagcaaatcc gcactcatct tattgatcat
    14161 cccgcgatat gccggcatca aacggttgat aaatgagtga tgatgaccac agacaaggag
    14221 gtcgcactgc tctttttgca taatttccag cagtgtttcc ggcatttctc cgcgttcaat
    14281 acgcagtttt gtcttcggcc attgaatatt tttcgtcagt ttatacagct tgttatccga
    14341 cttattcttc aacaattgaa gaatatcttc tgttgcaggg aagtagatac ccggatacaa
    14401 ctcgctcaag ccatcatcaa tatgaattaa cgtcaggtga gcgtcattat gtctggcgag
    14461 ctccagagct ttattcacca gtaaggcatc ttcttcattc ccggaaattg ccacgccaat
    14521 gtgtttataa gccatgttta actccttcta aagcaactcc atcaagctaa cgaacgcagt
    14581 gataactcaa aaataatcat ctcggcctgg catgagaaag tgaaggccgc atcaagctca
    14641 acttgcccct ccagatcctt aatcttgtag gccacgctgg ccccttcatc tgcagaggat
    14701 gcagcggcgg caagctcttt cagtttggtc ataaaagact ctgcttcttc gcgggtggca
    14761 aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa tcaccgtacc gatatcaatc
    14821 gcacatcctt tagtactgca tttatctacg acatctttca tagggcacct ctgtatattc
    14881 gcctctctgt cgattacgac ttaagaataa tgcgccgtta agggaagaac aaacaagtaa
    14941 tgatcttatg gattaccatt ttttttgata ttgcttaagg tgaagagttc agaaggattt
    15001 tccgggtatt gtcatttgca tcgtggaaat aacaatcccg gaaaaaaata gcagtaagag
    15061 atatattaaa ggttaattac ttgatttatt gtcggcttta tacttcacat cctgagtatc
    15121 tttaccatat ttattttcgc cttgtgtgcc aacaaacgca ccaagatcga taagcatcat
    15181 caccagaatc aacgtcggga caaaacgccc caccgcccat tgccagacac ccggtaaaat
    15241 cgcccagtta cccgccagca gcatccacgc cacaatcatc agaaatgccc atgcgccgga
    15301 acgcccgcga tcatgcaagc gcttaacagt tactgccgct gttggccaga gcaagcacac
    15361 aaggcaaaac gccgcggtct gaatatcgag tagattctta cccgccagtg agaaaagcac
    15421 cagcatgcct gcgaaccaca ggcctatcca aatccagaaa tcacggcgtc caatacgccc
    15481 tttaaatgag aataaccatt gctgtatggt catgtaagtt ccttgatggt tgtcttttcc
    15541 aggattctac ccttttgaca agggcgacag atatcgtttt aatcggagcc agtcataaca
    15601 aaaggtactg tcaatgaagc cagggtgtac gctgtttttt ctcttatgtt ctgcattaac
    15661 cgttacaaca acggcgcatg cacaaacacc agatacggca acgaccgcgc cttatctgct
    15721 ggctggagcc cctactttcg atctctccat cagccagttt cgagaagact ttaacagcca
    15781 gaatcctagc ctgccactga acgaatttcg tgccatcgac agcagtcccg acaaagccaa
    15841 tctcactcgt gctgcaagta aaattaacga gaacttgtat gcttctacag cgctggagcg
    15901 cggtacctta aaaatcaaaa gcattcaaat gacctggcta cccatccagg ggccagagca
    15961 aaaagcagcg aaagcgaaag ctcaggaata catggcagca gtgatccgca cactcacccc
    16021 attaatgacc aaaacacaaa gccagaaaaa actgcagtcg ctgctaacgg cggggaaaaa
    16081 caaacgttat tacaccgaga cagaaggtgc actgcgttat gttgtcgcgg acaacggcga
    16141 aaaggggctg accttcgctg ttgaaccgat taagctggcg ctatctgaat cgcttgaagg
    16201 tttgaataaa tgacaaaaag caaagccttt gtgccgatga atctctatac tgtttcacag
    16261 acctgctgcc ctgcggggcg gccatcttcc tttattcgct tataagcgtg gagaattaaa
    16321 atgcgacatc ctttagtgat gggtaactgg aaactgaacg gcagccgcca catggttcac
    16381 gagctggttt ctaacctgcg taaagagctg gcaggtgttg ctggctgtgc ggttgcaatc
    16441 gcaccaccgg aaatgtacat cgatatggcg aagcgcgaag ctgaaggcag ccacatcatg
    16501 ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat tcaccggtga aacttctgcc
    16561 gctatgctga aagacatcgg cgctcagtac atcatcatcg gccactctga acgtcgtact
    16621 taccacaaag agtctgacga actgatcgcg aaaaaattcg cggtgctgaa agagcagggc
    16681 ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa acgaagcggg caaaactgaa
    16741 gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc agggtgctgc ggcattcgaa
    16801 ggtgcggtta tcgcttacga acctgtatgg gcaatcggta ctggcaaatc tgcaactccg
    16861 gctcaggcac aggctgttca caaattcatc cgtgaccaca tcgctaaagt tgacgctaac
    16921 atcgctgaac aagtgatcat tcagtacggc ggctctgtaa acgcgtctaa cgctgcagaa
    16981 ctgtttgctc agccagatat cgacggcgcg ctggttggcg gtgcttctct gaaagctgac
    17041 gctttcgcag tgatcgttaa agctgcagaa gcggctaaac aggcttaagt ctgacaggtg
    17101 ccggatttca tatccggcac ttactttcct taactcttcg ccttaacgca aaatctcaca
    17161 ctgatgatcc tgaatttcct cggctgaagc acggttaagc gtcagtagat ttcgttgtgt
    17221 cgccagcaat acaaatgagt tatcactctg ccgtaccatc gccagcccgt agcttcccat
    17281 atgttcccgc gcctcaggta cttcttctgc cagcatcata aatgggctgc gttgtaccag
    17341 ttcgctttcc gttacccgac gcgccaggta ttcatgcccg cgcaaaccac ctggcagtgg
    17401 caaccagcgg ctgctgatgt tcgccagatt gttatccagt tgtttgcgca catcaggacg
    17461 aatacaagag atatgaatat gaaaatggtt ttgcgtacgc ccggtgcggg agttgatcgc
    17521 caaagaaacc gcgcgatcgg gaaccggctg gccgtatttt ttgctcataa aatcacgcgc
    17581 ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg gtcaacaaag gactttcagt
    17641 accgttaata cgatacgttg gcatcaacag atattgcagt gggccattaa gatcttttaa
    17701 aaccacgtat ccggcattgg gtttgacttc cgcacatggc gaaggatttt gattttgctg
    17761 ctgattgggc aaacattcct caaggacaat cttacgtaat gtatccgact cttcaccggt
    17821 taatttccag taaccaatac cggcagccac aacggcgata actatcatca ccaaaaaaag
    17881 aagacccgct tttttcatct ttttttccct gtacctcaaa gagatgcaag ggtaacgcaa
    17941 aatcgtgaca aataaaaaac ccggtcgcga gaaaccaccg ggtttgataa ttatcctgga
    18001 gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt agcaaaatgc tctttttgcg
    18061 ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa cttcagcttt ggaaacgcat
    18121 tttcgtactt tttcgctact tcagcgtcgc gcggacggta gtagtttttc gcggcaattt
    18181 cctgaccttc tggcgagtag agatatttca ggtaggcttc cgccacctct ttggtgcctt
    18241 ttttctcgac cactttatcg accaccgaca cggttggctc tgcgaggata gactcactcg
    18301 gcgtgacgat ttcgaattta tctttcccca gttcattcgc tgccagcaga gcttcgtttt
    18361 cccaagcgat cagtacatcg ccaatcccgc gctcgacaaa ggtgttagtg gagccgcgcg
    18421 cgccagaatc cagaacttcg acgtttttat acagtgcccg aacaaaatcc tgtgcttttg
    18481 cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc agccaggtag ttccagcgtg
    18541 cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac acccggttta atcagatcgt
    18601 tccagtcatg gatctgcttc ggattgccct tacgcaccag gaaaacaatg gtggaagtgt
    18661 acggtgcgga gttatccggc agacgtttga tccactcttt atcaatccgc ccgcgttccg
    18721 caatcgcgtc cacgtcatag gccagagcca gcgtgacaac atcagcttca ataccgttga
    18781 ttaccgacgt cgcttgttta cctgaaccac cgtgtgactg acgaatcacc acgttttcac
    18841 cagtttgctg tttccagtgg gcgctgaatg ccttgttgta ctgttcgtac aattcgcgcg
    18901 ttggatcata tgaaacgtta agaagctgaa tatcctttgc cataacgttg gttgccgcca
    18961 gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg actctcttat gttgtgttgt
    19021 gatgagcaaa gcgtgccaga aggttaacca aacattaaag aataaaaaaa gattggctat
    19081 aacttgcggg tatatgttgg gggttaaaaa ggcggaagaa atccgcctca tattgctgac
    19141 aaagtgcgct ttatcgatgc cggatgcggc gcgaacgcct tatccggcct acaaaaattt
    19201 gtaaattcaa taaattgcag aattcacgta ggcctgataa gcgaagcgca tcaggcattt
    19261 ttgcttctgt catcggtttc aggctaaagg aatctgcctt tttccgaaat cattaataca
    19321 gttttttcgc gcagtccagc cagtcgcctt tgaacggacg cttcatgttt tcgatagcgt
    19381 cgatgatgtc gtggtgaacc agctgttcgt tctggatacc tacgcaacga ccgccgtaac
    19441 ctgccagcag cagatcgata gcgtaagcgc ccatacggga agccagaata cggtcgtaag
    19501 gcaccggaga accaccgcgc tggatgtggc ccagcacagt tgcgcgggtt tcacgaccgg
    19561 tttctttctc gatgaaatgc gccagttcgt caacatcaca catatgttcg gtaatcgcca
    19621 cgatcgcgtg ttttttacct ttcgcgatac ccgctttgat ttcgtttacc agatcttcac
    19681 ggctgaattc aacttccgga accacaacga attcacagcc accggcaatg gccgcagcca
    19741 acgtcagatc gccacaataa cggcccatca cttccaccac ggaaatacgc tggtgagaag
    19801 aagaggtgtc acgcagacgg tcgatcgctt ctacaacggt gctcagcgca gtgaagaaac
    19861 cgatagtgta gtcagtgcct ttgatgtcgt tgtcgatagt gccaggcaga ccgatgcacg
    19921 ggaagcccat ttcggtcaga cgcattgcac ccatgtagga accgtcaccg ccgataacca
    19981 ccagcgcgtc gataccacgt tttttcaggt tttcgatagc cacggcgcgg atgttctcgt
    20041 cgcggaattc cgggaaacgc gcagaaccga ggaacgtacc accacggttg atcatgtcag
    20101 aaacgctgta acggtctagc tgtaccatac ggtcttcata cagacccaga tagccgtcat
    20161 aaatgcccat tacttccaga ccttctgtca gcgcagaacg aacaaccccg cgaattgcgg
    20221 cgttcatgcc tggcgcatca ccgccgcttg tcaacacacc gattttctta atcatgacta
    20281 cctctgaact ttggaatgca aaataaaatc tgttgccgga agtcttcttg cacatcgaag
    20341 tgatccaacg aatgtgcaaa tagtataaca atcacttcct gctgaattga ttcaggtcag
    20401 gccaaatggc ggtattttat acacaaaatg cgggtctggc tctcttttat actgattatg
    20461 aaagcataga ccgtttaccc tccctgggta cgacggaaca ggggtcctga tggataatta
    20521 catccgatcc cggaaaacgc cgtaaaatag cctgctctac ctgatccgcc accatatgtg
    20581 cctgaaccaa aggcagagag tcttccattt ccaaatgaat ctgaataaag cgggtcggcc
    20641 ctgactgccg cgtgcgaaga tcgtgagcgc cgctaacacc cggccaggaa gtcacgatat
    20701 caataatttc ttgccgttcc tcatcaggca atgcgcgatc cagtaatgac tgtaccgcct
    20761 catatcccat gcgtaacgcg ctatataaaa tatagatgcc gattcccaat gcaaacagag
    20821 catcggcgcg atgccagccg taccaggaca accccagcgc cagcagaatt gcgccgttca
    20881 tcataacatc agactggtaa tgtagcatat cagcccgcac cgcctggctt tgcgtccggc
    20941 gcaccaccca acgctgaaac gagacaagga taatcgtaca aattagcgcc acaattgtca
    21001 cgataacccc gacgcctgga tctgtcatcg gtgttggaga tatcagatgt tgaatacccg
    21061 tcaaaaacag gaatagtgcc gaaccggaga taaacatact ttgcgccagc gccgcgaggg
    21121 actctgcttt accgtgacca aacgagtgat tatcgtcggc aggttgcagg gaatatcgca
    21181 ccaccagtaa attcgtcaac gacgcgccga tatccaccag cgaatccacc agcgcggcga
    21241 gaatactcac cgacccggta taccaccatg caaaaatttt aatcagcaat agcaacgaag
    21301 ccatcgccgt cgcagcaatc gccgcccgac tgaccagccg tccataagat tgattcataa
    21361 atactcccgc tatcaactga cgctagtata acggaagcaa atcatctgca atgcattaag
    21421 cagcaggcaa attgaggata aaaaaaaccc ccacatcatg tgggggaaga cagggatggt
    21481 gtctatggca aggaaaacag ggtttactac tgggaacgtg agttgctact actcaatagc
    21541 ttcaacgatg aacttttttg ccattgcgtc acgtcacgca actgctccat tcgttgttga
    21601 tgtttctcgt ttaaaaccgc ttgctgctcc ggcgttaaca ggcgatacat ttggttgcgg
    21661 acttttgcca tctcaacctg acgagcaatt tgctcattcg ccattttttc tgcctgtgcg
    21721 cgcacagcgt tttcatcaaa attttctgcg gtgacaaggc gatgcattgt ctccagttcg
    21781 ctaacattaa caggaggctg ttcgtgccgg gcctgttgca taagatctcg catctgctga
    21841 cgctgatgtt cggttaaact tatgccgtcg aacatatggc tctgcgtact gcgctgcgta
    21901 agttcttcac ccggatgcca gttatcgcct gaaccgactt cagcagcgtg gcttaatgaa
    21961 ctgactgcca gcgttgaggc catgacggca gcggtaacta tgcgcatcat ttgctcccaa
    22021 aatctttctg tcgcgattca acgatagaga gtttacgatt caggctgcaa acatgcgtca
    22081 gggggtgtaa aacaacgtaa agtcatggat tagcgacgtc tgatgacgta atttctgcct
    22141 cggaggtatt taaacaatga ataaaatcct gttagttgat gatgaccgag agctgacttc
    22201 cctattaaag gagctgctcg agatggaagg cttcaacgtg attgttgccc acgatgggga
    22261 acaggcgctt gatcttctgg acgacagcat tgatttactt ttgcttgacg taatgatgcc
    22321 gaagaaaaat ggtatcgaca cattaaaagc acttcgccag acacaccaga cgcctgtcat
    22381 tatgttgacg gcgcgcggca gtgaacttga tcgcgttctc ggccttgagc tgggcgcaga
    22441 tgactatctc ccgaaaccgt ttaatgatcg tgagctggtg gcacgtattc gcgcgatcct
    22501 gcgccgttcg cactggagcg agcaacagca aaacaacgac aacggttcac cgacactgga
    22561 agttgatgcc ttagtgctga atccaggccg tcaggaagcc agcttcgacg gacaaacgct
    22621 ggagttaacc ggcactgagt ttaccctgct ctatttgctg gcacagcatc tgggtcaggt
    22681 ggtttcccgt gaacatttaa gccaggaagt gctgggcaaa cgcctgacgc ctttcgaccg
    22741 cgctatcgat atgcacattt ccaacctgcg tcgtaaactg ccggatcgta aagatggtca
    22801 cccgtggttt aaaaccttgc gtggtcgcgg ctatctgatg gtttctgctt catgataggc
    22861 agcttaaccg cgcgcatctt cgccatcttc tggctgacgc tggcgctggt gttgatgttg
    22921 gttttgatgt tacccaagct cgattcacgc cagatgaccg agcttctgga tagcgaacag
    22981 cgtcaggggc tgatgattga gcagcatgtc gaagcggagc tggcgaacga tccgcccaac
    23041 gatttaatgt ggtggcggcg tctgttccgg gcgattgata agtgggcacc gccaggacag
    23101 cgtttgttat tggtgaccac cgaaggccgc gtgatcggcg ctgaacgcag cgaaatgcag
    23161 atcattcgta actttattgg tcaggccgat aacgccgatc atccgcagaa gaaaaagtat
    23221 ggccgcgtgg aactggtcgg tccgttctcc gtacgtgatg gcgaagataa ttaccaactt
    23281 tatctgattc gtccggccag cagttctcaa tccgatttca ttaacttact gtttgaccgc
    23341 ccgttattac tgctgattgt caccatgttg gtcagtacgc cgctgctgtt gtggttggcc
    23401 tggagtctgg caaaaccggc gcgtaagctg aaaaacgctg ccgatgaagt tgcccaggga
    23461 aacttacgcc agcacccgga actggaagcg gggccacagg aattccttgc cgcaggtgcc
    23521 agttttaacc agatggtcac cgcgctggag cgcatgatga cctctcagca gcgtctgctt
    23581 tctgatatct ctcacgagct gcgcaccccg ctgacgcgtc tgcaactggg tacggcgtta
    23641 ctgcgccgtc gtagcggtga aagcaaggaa ctggagcgta ttgaaaccga agcgcaacgt
    23701 ctggacagca tgatcaacga tctgttggtg atgtcccgta atcagcaaaa aaacgcgctg
    23761 gttagcgaaa ccatcaaagc caaccagttg tggagtgaag tgctggataa cgcggcgttc
    23821 gaagccgagc aaatgggcaa gtcgttgaca gttaacttcc cgcctgggcc gtggccgctg
    23881 tacggcaatc cgaacgccct ggaaagtgcg ctggaaaaca ttgttcgtaa tgctctgcgt
    23941 tattcccata cgaagattga agtgggcttt gcggtagata aagacggtat caccattacg
    24001 gtggacgacg atggtcctgg cgttagcccg gaagatcgcg aacagatttt ccgtccgttc
    24061 tatcggaccg atgaagcacg cgatcgtgaa tctggcggta caggattggg gctggcgatt
    24121 gttgaaaccg ccattcagca gcatcgtggc tgggtgaagg cagaagacag cccgctgggc
    24181 ggtttacggc tggtgatttg gttgccgctg tataagcgga gttaaactcc gcatttgtag
    24241 gcaggataag gcgtttacgc cgcatccggc atttgagcag gatgcctgat gcgacgctga
    24301 tagcgtctta tcaggcctac actccggggg ttattttccc cacaaccggc gggaaaaatc
    24361 ttcgatcttg ccgctcagtc ggcgcttttg catcgtccgc gtccagcttt tcgataaccc
    24421 ggcggcggag agtaaacggt gatactgatc gtcatcaaac ggcatatgcc atgcgatggc
    24481 ggcagcctcc tgcacggtca catcgctgac acgggaaacc aactccagcg gcgcgtccgc
    24541 agacactttc cctggcgcta tcacgctata caaccacccc accttacctg tgttttgcat
    24601 caactgcgct atatcgctga tatcaaaatg gtaattgagt ttatagcaag gcgagcgcgg
    24661 ctggctgacc tgaattaatg cctctcccca gcggaaaata tcgcccatat gaacattact
    24721 ttccgtgaga ccgtcggttg agagattttc gccaaacgca ggcgcaacaa acaactccgc
    24781 ctgttccgga aattcccgca cccagtagag atagtgctca cgaggataat gacacagcgc
    24841 tctgtctggc ccgccgtgaa cctttttctc cgcctgctcg tcaccttcca gccccagctc
    24901 cgtcagcatc aactcaccat caacctggat tttggcaatt gcgctgggtt tgccttcggg
    24961 ataagcctga atcttgcctg tgtatacatc aaccggatat cgcatctgtt ccctctccac
    25021 gagaaatttc cccattagac catatttatt ttatgtcagg cgtgctgttt tcgcggtaag
    25081 tatccaggca ggaactcaat agcaaaccgg acgaacgtta gttcatccgg ctgtggggat
    25141 taaggaagca ggtttatttc accgtaccta aaatttcgat tttcgctgct ctggctttga
    25201 ctttacgtga ccagatagag gtaataattg gcaccagaat cgaagtcaca atgaccgccg
    25261 tcgctaccag cgaagttgct gccggagcca tcggtttaaa cgcaggcacc atttctgcaa
    25321 tcagcacagg tgtcgctacc gcggcccctg cggaactgga agcggcaatt ccggcagtgc
    25381 catcgccacc gccaatcaat ttatcggcga taatcaacgg aataccggtc acgataatta
    25441 ctgccacacc caacaggatc cccagcaaac cagtctgggc aattacagtc aaatcaatgg
    25501 tattgcccag tgcgaaggca aagaatggaa tcagcgtctg caccgctttg ctgaaaaatt
    25561 ctcgtaattc agggtcaagg ttccccaggg caaagcccac caggaacggt aatactgcgc
    25621 cgacgaaaac atgcggttca aacgaggcaa tccctgcagt gcccagaata atcatcgtca
    25681 tgagcggccc ggactccaac gacatcaaca caaatgcccc agcttcttct tttgtgccgt
    25741 actgctgcat gatggaagcg taaagtccgc cattggtcat atccatcgcc gccaccagcg
    25801 ccagcgttga aagtccggca aagaatccaa cttcaacacc atgttccgga ataatgcgcg
    25861 aggcaattgc cgcaaccacc cacgcgacgg caatttttgt taccaccagc gtaccggatt
    25921 tacgcaatac cgttcccgtc gcgcttaatt ttattgacgc ccccatgcaa aaaaaccaca
    25981 ccgccagaat gggcaccgta ccggtaatca tcccgttggt gaatgatcca aaatatttcc
    26041 ccgcccccgg cgagaaggtg tggcacagtg cgccaaggaa tagcgggacg agcatcatcc
    26101 cccccgggat tttctcaatc gagcgtttta tctgcatttc catcacctgt cggcatattt
    26161 ttaagggctg atgcgtcatt tctcaatgga gcatcagtgt tgatggaaac gaagatagac
    26221 attcatttaa tcaataaaag tgatcaaaac cacatcaatt gaaacactgt tttattttta
    26281 tcggatcatt gtttttattt tgaaattgat cacaaaaaaa ccaccgccgt tggcgatggt
    26341 tcatttttta agctgatatg cggcgtaaac gcctcattgc agctggcggc aaatgattat
    26401 tttttcgccg cgaaacgcgc cgcagcttcg tcccagttca ccacgttcca gaactctttg
    26461 atgtagtccg ggcggcggtt ctggaatttc aggtagtaag catgttccca cacatccagg
    26521 cccagaatcg ggaagccgga agcgccagaa atagcttcac ccatcagcgg agaatcctgg
    26581 ttagcagtag aaaccaccgc cagtttatcg cctttcagca ccagccatgc ccagccggaa
    26641 ccaaagcggg aagctgccgc tttttcaaat tctgctttga agttatcaac ggagccgaag
    26701 tcacgttcga tagccgcttt caggtcaccc tgcagggtgg tgcctttttt cagacctttc
    26761 cagaacaggc tgtggttagc gtgaccgcca gcgttgttgc gcagtacggt tttcttgtct
    26821 gctggcagct ggtccagttt ggtgatcagc tcttcaaccg gcaggttggc aaattctggc
    26881 aggctttcca gcgccgcgtt ggcgttgttt acgtaggtct gatggtgttt ggtgtggtgg
    26941 atttccatgg tctgcttatc gaagtgcggt tccagggcat cgtaagcata cggcagggat
    27001 ggcagggtat agctcatatt catctccagt attgtcgggc ggccgattgt taatgccgcg
    27061 taagcagttg gttcattata gttaattaaa tgatattgaa aatgattatc aatgccgtac
    27121 ttttcgtaag ggtatggttt tgcaggaaaa tgcccgagat gtgaagcaaa tcacccactt
    27181 aatgccgtga ttgccagtaa atcgacaacg gcggcaacag gcgaaaggtt aatcgacagc
    27241 acgattttta cactcatctc gtcggagatg tgaagcgacg aaaaatgatg aggataagaa
    27301 gatgagtaac gcgattacga tggggatatt ttggcatttg atcggcgcgg ccagtgcagc
    27361 ctgtttttac gctccgttca aaaaagtaaa aaaatggtca tgggaaacca tgtggtcagt
    27421 cggtgggatt gtttcgtgga ttattctgcc gtgggccatc agcgccctgt tactaccgaa
    27481 tttctgggcg tattacagct cgtttagtct ctctacgctg ctgcctgttt ttctgttcgg
    27541 cgctatgtgg gggatcggta atatcaacta tggcctgacc atgcgttatc tcggcatgtc
    27601 gatgggaatt ggcatcgcca ttggcattac gttgattgtc ggtacgctga tgacgccaat
    27661 tatcaacggc aatttcgatg tgctgattaa caccgaaggc ggacgcatga cgttgctcgg
    27721 cgttctggtg gcgctgattg gcgtagggat tgtaactcga gccgggcagt tgaaagagcg
    27781 caagatgggc attaaagccg aagagttcaa tctgaaaaaa gggctggtgc tggcggtgat
    27841 gtgcggcatt ttctctgccg ggatgtcctt tgcgatgaac gccgcaaaac cgatgcatga
    27901 agccgctgcc gcactaggcg tcgatccact gtatgtcgct ctgccaagct atgtcatcat
    27961 catgggcggc ggcgcgatca tcaacctcgg tttctgtttt attcgtctgg caaaagtgaa
    28021 ggatttgtcg ctaaaagccg acttctcgct ggcaaaaccg ctgatcactc acaatgtgtt
    28081 actctcagca ctgggcgggt tgatgtggta tctgcaattc tttttctatg cctggggcca
    28141 cgcccgcatt ccggcgcaat atgactacat cagttggatg ctgcatatga gcttctatgt
    28201 attgtgcggc ggtatcgtcg ggctggtgct gaaagagtgg aacaatgcag gacgccgtcc
    28261 ggtaacggtg ttgagcctcg gttgtgtggt gattattgtc gccgctaaca tcgtcggcat
    28321 cggcatggcg aattaatctt tctgcgaatt gagatgacgc cactggctgg gcgtcatccc
    28381 ggtttcccgg gtaaacacca ccgaaaaata gttactatct tcaaagccac attcggtcga
    28441 aatatcactg attaacaggc ggctatgctg gagaagatat tgcgcatgac acactctgac
    28501 ctgccgcaga tattgattga tggtcattcc agtctgctgg cgaaattgct gacgcaaaac
    28561 gcgctcactg cacgatgcct catcacaaaa tttatccagc gcaaagggac ttttcaggct
    28621 agccgccagc cgggtaatca gcttatccag caacgtttcg ctggatgttg gcggcaacga
    28681 atcactggtg taacgatggc gattcagcaa catcaccaac tgcccgaaca gcaactcagc
    28741 catttcgtta gcaaacgaca catgctgact actttcatgc tcaagctgac cgataacctg
    28801 ccgcgcctgc gccatcccca cgctacctaa gcgccagtgt ggttgccctg cgctggcgct
    28861 aaatcccgga atcgctccct gccagtcaag attcagcttc agccgctccg ggcaataaat
    28921 aatattctgc aaaaccagat cgttaacgga agcgtaggag tgtttatcgt cagcatgaat
    28981 gtaaaagaga tcgccacggg taatgcgata agggcgatcg ttgagtacat gcagaccatt
    29041 accgcgccag acaatcacca gctcacaaaa atcatgtgta tgttcggcaa aaacatcttg
    29101 cggataacgg tcagccacag cgactgcctg ctggtcgctg gcaaaaaaat catctttgag
    29161 aagttttaac tgatgcgcca ccgtggctac ctcggccaga gaacgaagtt gattattcgc
    29221 aatatggcgt acaaatacgt tgagaagatt cgcgttattg cagaaaacca tcccgtccct
    29281 gacgaatatc acgcggtgac cagttaaact ctcggcgaaa aagcgtcgaa aagtggttac
    29341 tgtcgctgaa tccacagcga taggcgatat cagtaacgct ggcctcgctg tggcgtagta
    29401 gatgtcgggc tttcatcagt cgcaggcggt tcaggtaacg ctgaggcgtc agtcccgttt
    29461 gctgcttaag ctgccgatgt agcgtacgca gcgaaagaga aaattgctcc gccacggcat
    29521 cccaattcac ctcatcggca aaatggtcct ccagccaggc cagaagcaag ttgagacgtg
    29581 atgcgctgtt ttccaggttc tcctgcaaac tgcttttacg cagcaagagc agtaattgca
    29641 taaacaagat ctcgcgactg gcggtcgagg gtaaatcatt ttccccttcc tgctgttcca
    29701 tctgtgcaac cagctgtcgc acctgctgca atacgctgtg gttaacgcgc cagtgggaag
    29761 gatactgccc atccagctct tgtggcagca actgattcag cccggcgaga aactgaaatc
    29821 gatccggcga gcgatacagc acattagtca gacacagatt atcggtatgt tcatacagat
    29881 gccgatcatg gtcgcgcaca aaacagaccg tgccaccggt gatggtatag ggctgcccat
    29941 taaacacatg aatacccgtg ccatgttcga caatcacaat ttcatgaaaa tcatgatgat
    30001 gttcaggaaa atccgcctgc gggagccggg gttctatcgc cacggacgcg ttaccagacg
    30061 gaaaaaaatc cacactatgt aatacggtca tactggcctc ctgatgtcgt caacacggcg
    30121 aaatagtaat cacgaggtca ggttcttacc ttaaattttc gacggaaaac cacgtaaaaa
    30181 acgtcgattt ttcaagatac agcgtgaatt ttcaggaaat gcggtgagca tcacatcacc
    30241 acaattcagc aaattgtgaa catcatcacg ttcatctttc cctggttgcc aatggcccat
    30301 tttcctgtca gtaacgagaa ggtcgcgaat tcaggcgctt tttagactgg tcgtaatgaa
    30361 attcagcagg atcacattat gacctttcgc aattgtgtcg ccgtcgatct cggcgcatcc
    30421 agtgggcgcg tgatgctggc gcgttacgag cgtgaatgcc gcagcctgac gctgcgcgaa
    30481 atccatcgtt ttaacaatgg gctgcatagt cagaacggct atgtcacctg ggatgtggat
    30541 agcctggaaa gtgccattcg ccttggatta aacaaggtgt gcgaggaagg gattcgtatc
    30601 gatagcattg ggattgatac ctggggcgtg gactttgtgc tgctcgacca acagggtcag
    30661 cgtgtgggcc tgcccgttgc ttatcgcgat agccgcacca atggcctgat ggcgcaggca
    30721 caacaacaac tcggcaaacg cgatatttat caacgtagcg gcatccagtt tctgcccttc
    30781 aatacgcttt atcagttacg ggcgctgacg gagcaacaac ctgaacttat tccacacatt
    30841 gctcacgctc tgctaatgcc ggattatttt agttatcgcc ttaccggcaa gatgaactgg
    30901 gagtacacca acgccacaac cacgcaactg gtcaatatca atagcgacga ctgggacgag
    30961 tcgctactgg cgtggagcgg tgccaacaaa gcctggtttg gtcgcccgac gcatccgggt
    31021 aatgtcatag gtcactggat ttgcccgcag ggtaatgaga ttccggtggt cgccgttgcc
    31081 agccatgata ccgccagcgc ggttatcgcc tcgccgttaa acggttcacg cgccgcttat
    31141 ctctcttctg gtacctggtc attgatgggc ttcgaaagcc agacgccatt taccaatgac
    31201 acggcgctgg cagccaacat caccaatgaa ggcggggcgg aaggtcgcta tcgggtgctg
    31261 aaaaatatta tgggcttatg gctgcttcag cgggtgctta aggagcggaa aatcaacgat
    31321 ctcccggcgc ttatcgtcgc gacacagtca cttccggcct gccgcttcat catcaatccc
    31381 aatgacgatc gctttattaa tcctgaggcg atgtgcagcg aaattcaggc tgcgtgtcgg
    31441 gaaacggcgc aaccgatccc ggaaagtgat gctgaactgg cgcgctgcat tttcgacagt
    31501 ctggcgctgc tgtatgccga tgtgttgcat gagctggcgc agctacgcgg tgaagatttc
    31561 tcgcaactgc atattgtcgg cggaggctgt cagaacacgc tgctcaacca gctatgtgcc
    31621 gatgcctgcg gtattcgggt gatcgccggg cctgttgaag cctcgacgct cggcaatatc
    31681 ggcatccagt taatgacgct ggatgaactc aacaatgtgg atgatttccg tcaggtcgtc
    31741 agcaccaccg cgaatctgac cacctttacc cctaatcctg acagtgaaat tgcgcactac
    31801 gtggcgcgga ttcactctac acgaaagaca aaggagcttt gcgcatgacc actcaactgg
    31861 aacaggcctg ggagctggcg aaacagcgtt tcgcggcggt ggggattgat gtcgaggagg
    31921 cgctgcgcca acttgatcgt ttacccgttt caatgcactg ctggcagggc gatgatgttt
    31981 ccggttttga aaacccggaa ggttcgctga ccggggggat tcaggccaca ggcaattatc
    32041 cgggcaaagc gcgtaatgcc agtgagctac gtgccgatct ggaacaggct atgcggctga
    32101 ttccggggcc gaaacggctt aatttacatg ccatctatct ggaatcagat acgccagtct
    32161 cgcgcgacca gatcaaacca gagcacttca aaaactgggt tgaatgggcg aaagccaatc
    32221 agctcggtct ggattttaac ccctcctgct tttcgcatcc gctaagcgcc gatggcttta
    32281 cgctctcgca tcccgacgac agcattcgcc agttctggat tgatcactgc aaagccagcc
    32341 gtcgcgtttc ggcctatttt ggcgagcaac tcggcacacc atcggtgatg aacatctgga
    32401 tcccggatgg tatgaaagat atcaccgttg accgtctcgc cccgcgtcag cgtctgctgg
    32461 cagctctgga tgaggtgatc agcgagaagc tggatccggc gcaccatatc gacgccgttg
    32521 agagcaaatt gtttggcatt ggcgcagaga gctacacggt tggctccaat gagttttaca
    32581 tggggtatgc caccagccgc cagactgcgc tatgcctgga cgccgggcac ttccacccga
    32641 ctgaagtgat ttccgacaag atttccgccg ccatgctgta tgtgccgcag ttgctgctgc
    32701 acgtcagccg tccggttcgc tgggacagcg atcacgtagt gctgctggat gatgaaaccc
    32761 aggcaattgc cagtgagatt gtgcgtcacg atctgtttga ccgggtgcat atcggccttg
    32821 acttcttcga tgcctctatc aaccgcattg ccgcgtgggt cattggtaca cgcaatatga
    32881 aaaaagccct gctgcgtgcg ttgctggaac ctaccgctga gctgcgcaag ctggaagcgg
    32941 cgggcgatta cactgcgcgt ctggcactgc tggaagagca gaaatcgttg ccgtggcagg
    33001 cggtctggga aatgtattgc caacgtcacg atacgccagc aggtagcgaa tggctggaga
    33061 gcgtgcgggc ttatgagaaa gcgattttga gccagcgtgg gtaaacactg ccggatgcgg
    33121 cgcgagcgcc ttatccggcc tacgggtcgt gcatctgaca acaccgaatt tacaggaaca
    33181 cagaacatgc aaaacattac ccagtcctgg tttgtccagg gaatgatcaa agccaccacc
    33241 gatgcctggc tgaaaggctg ggacgagcgc aacggcggca acctgacgct gcgcctggat
    33301 gacgccgata tcgcgcttta tcacgacaac ttccacccac aaccgcgcta tattccgctc
    33361 agccagccca tgcctttact ggcaaatacg ccgtttattg tcaccggctc cggcaaattc
    33421 ttccgtaacg tccagcttga tcctgcagct aacttaggcg tcgtaaaagt cgacagcgac
    33481 ggcgcgggct accatattct ctgggggtta accaacgaag ccgttcccac ttccgaactt
    33541 ccggctcact tcctttccca ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg
    33601 atcatgcact gccacgccac caacctgatc gccctcacct atgtactgga aaacgacacc
    33661 gcggtcttta ctcgccaact gtgggaaggc agcaccgagt gtctggtagt gttcccggat
    33721 ggcgttggca ttttgccgtg gatggtgccc ggcacggacg aaatcggcca ggcgaccgca
    33781 caagagatgc aaaaacattc gctggtgttg tggtccttcc acggcgtctt cggcagcggg
    33841 ccgacgctgg atgaaacatt cggtttaatc gacaccgcag aaaaatcagc acaagtatta
    33901 gtgaaggttt attcgatggg cggcatgaaa cagaccatca gccgtgaaga gttgatagcg
    33961 ctcggcaagc gcttcggcgt tacaccacta gccagtgcgc tggcgctgta aggagcaaac
    34021 atgatccgca aagcctttgt catgcaggtt aaccccgacg cccacgaaga gtatcagcgt
    34081 cggcataacc ccatctggcc ggaactggaa gcagtgttga aatctcacgg tgcgcataac
    34141 tacgccattt acctcgacaa agcgcgtaat ctgctgtttg ccacggtaga gattgaatct
    34201 gaagaacggt ggaatgcggt agccagtact gatgtttgcc aacgttggtg gaaatatatg
    34261 accgatgtta tgcccgctaa cccggataac agcccggtga gtagcgagct gcaagaagtg
    34321 ttttacctgc cgtaattgcc caatgtg
//
LOCUS       DAJOWB010000040        33585 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJOWB010000040 DAJOWB010000000
VERSION     DAJOWB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.040"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            37..1551
                     /gene="norR"
                     /locus_tag="OHO85_002105"
     CDS             37..1551
                     /gene="norR"
                     /locus_tag="OHO85_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCQ2671480.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLSRATRNGDEVILEAQHFAFPEVTLPTPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            complement(1548..2513)
                     /gene="gutQ"
                     /locus_tag="OHO85_002106"
     CDS             complement(1548..2513)
                     /gene="gutQ"
                     /locus_tag="OHO85_002106"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCQ2671481.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(2506..3279)
                     /gene="srlR"
                     /locus_tag="OHO85_002107"
                     /gene_synonym="gutR"
     CDS             complement(2506..3279)
                     /gene="srlR"
                     /locus_tag="OHO85_002107"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCQ2671482.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            complement(3346..3705)
                     /gene="gutM"
                     /locus_tag="OHO85_002108"
     CDS             complement(3346..3705)
                     /gene="gutM"
                     /locus_tag="OHO85_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCQ2671483.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            complement(3810..4589)
                     /gene="srlD"
                     /locus_tag="OHO85_002109"
     CDS             complement(3810..4589)
                     /gene="srlD"
                     /locus_tag="OHO85_002109"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCQ2671484.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            complement(4593..4964)
                     /gene="srlB"
                     /locus_tag="OHO85_002110"
                     /gene_synonym="gutB"
     CDS             complement(4593..4964)
                     /gene="srlB"
                     /locus_tag="OHO85_002110"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCQ2671485.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            complement(4975..5934)
                     /gene="srlE"
                     /locus_tag="OHO85_002111"
                     /gene_synonym="gutE"
     CDS             complement(4975..5934)
                     /gene="srlE"
                     /locus_tag="OHO85_002111"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCQ2671486.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            complement(5931..6494)
                     /gene="srlA"
                     /locus_tag="OHO85_002112"
                     /gene_synonym="gutA"
     CDS             complement(5931..6494)
                     /gene="srlA"
                     /locus_tag="OHO85_002112"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCQ2671487.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAVFEKKMGIQLEQKVHLAGATS"
     gene            6750..7835
                     /gene="mltB"
                     /locus_tag="OHO85_002113"
     CDS             6750..7835
                     /gene="mltB"
                     /locus_tag="OHO85_002113"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCQ2671488.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7980..8477
                     /gene="pncC"
                     /locus_tag="OHO85_002114"
     CDS             7980..8477
                     /gene="pncC"
                     /locus_tag="OHO85_002114"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCQ2671489.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            8557..9618
                     /gene="recA"
                     /locus_tag="OHO85_002115"
     CDS             8557..9618
                     /gene="recA"
                     /locus_tag="OHO85_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCQ2671490.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            9861..10361
                     /gene="recX"
                     /locus_tag="OHO85_002116"
     CDS             9861..10361
                     /gene="recX"
                     /locus_tag="OHO85_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCQ2671491.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLVAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            10489..13119
                     /gene="alaS"
                     /locus_tag="OHO85_002117"
     CDS             10489..13119
                     /gene="alaS"
                     /locus_tag="OHO85_002117"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCQ2671492.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            13354..13539
                     /gene="csrA"
                     /locus_tag="OHO85_002118"
     CDS             13354..13539
                     /gene="csrA"
                     /locus_tag="OHO85_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCQ2671493.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            13855..13947
                     /locus_tag="OHO85_002119"
     tRNA            13855..13947
                     /locus_tag="OHO85_002119"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13889..13891,aa:Ser,seq:gct)
     gene            13951..14027
                     /locus_tag="OHO85_002120"
     tRNA            13951..14027
                     /locus_tag="OHO85_002120"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13985..13987,aa:Arg,seq:acg)
     gene            14225..14301
                     /locus_tag="OHO85_002121"
     tRNA            14225..14301
                     /locus_tag="OHO85_002121"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14259..14261,aa:Arg,seq:acg)
     gene            14365..14441
                     /locus_tag="OHO85_002122"
     tRNA            14365..14441
                     /locus_tag="OHO85_002122"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14399..14401,aa:Arg,seq:acg)
     gene            14640..14716
                     /locus_tag="OHO85_002123"
     tRNA            14640..14716
                     /locus_tag="OHO85_002123"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14674..14676,aa:Arg,seq:acg)
     gene            14997..15563
                     /gene="yqaB"
                     /locus_tag="OHO85_002124"
     CDS             14997..15563
                     /gene="yqaB"
                     /locus_tag="OHO85_002124"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCQ2671494.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVGKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            15560..15988
                     /locus_tag="OHO85_002125"
     CDS             15560..15988
                     /locus_tag="OHO85_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCQ2671495.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            16061..17617
                     /gene="gshA"
                     /locus_tag="OHO85_002126"
     CDS             16061..17617
                     /gene="gshA"
                     /locus_tag="OHO85_002126"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCQ2671496.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            17767..18282
                     /gene="luxS"
                     /locus_tag="OHO85_002127"
     CDS             17767..18282
                     /gene="luxS"
                     /locus_tag="OHO85_002127"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCQ2671497.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGKGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(18346..19884)
                     /gene="emrB"
                     /locus_tag="OHO85_002128"
     CDS             complement(18346..19884)
                     /gene="emrB"
                     /locus_tag="OHO85_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCQ2671498.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(19901..21073)
                     /gene="emrA"
                     /locus_tag="OHO85_002129"
     CDS             complement(19901..21073)
                     /gene="emrA"
                     /locus_tag="OHO85_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCQ2671499.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(21200..21730)
                     /gene="emrR"
                     /locus_tag="OHO85_002130"
     CDS             complement(21200..21730)
                     /gene="emrR"
                     /locus_tag="OHO85_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCQ2671500.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(21821..22156)
                     /gene="ygaH"
                     /locus_tag="OHO85_002131"
     CDS             complement(21821..22156)
                     /gene="ygaH"
                     /locus_tag="OHO85_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCQ2671501.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALTYG
                     LVWKVMAII"
     gene            complement(22146..22883)
                     /gene="ygaZ"
                     /locus_tag="OHO85_002132"
     CDS             complement(22146..22883)
                     /gene="ygaZ"
                     /locus_tag="OHO85_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCQ2671502.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(23007..24191)
                     /locus_tag="OHO85_002133"
     CDS             complement(23007..24191)
                     /locus_tag="OHO85_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2671503.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(24483..25475)
                     /gene="proX"
                     /locus_tag="OHO85_002134"
     CDS             complement(24483..25475)
                     /gene="proX"
                     /locus_tag="OHO85_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCQ2671504.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(25533..26597)
                     /gene="proW"
                     /locus_tag="OHO85_002135"
     CDS             complement(25533..26597)
                     /gene="proW"
                     /locus_tag="OHO85_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCQ2671505.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(26590..27792)
                     /gene="proV"
                     /locus_tag="OHO85_002136"
     CDS             complement(26590..27792)
                     /gene="proV"
                     /locus_tag="OHO85_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCQ2671506.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(28147..29106)
                     /gene="nrdF"
                     /locus_tag="OHO85_002137"
     CDS             complement(28147..29106)
                     /gene="nrdF"
                     /locus_tag="OHO85_002137"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCQ2671507.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(29116..31260)
                     /gene="nrdE"
                     /locus_tag="OHO85_002138"
     CDS             complement(29116..31260)
                     /gene="nrdE"
                     /locus_tag="OHO85_002138"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCQ2671508.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFHTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVELVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYKIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(31233..31643)
                     /gene="nrdI"
                     /locus_tag="OHO85_002139"
     CDS             complement(31233..31643)
                     /gene="nrdI"
                     /locus_tag="OHO85_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCQ2671509.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIAQKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(31640..31885)
                     /gene="nrdH"
                     /locus_tag="OHO85_002140"
     CDS             complement(31640..31885)
                     /gene="nrdH"
                     /locus_tag="OHO85_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCQ2671510.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(32133..32462)
                     /locus_tag="OHO85_002141"
     CDS             complement(32133..32462)
                     /locus_tag="OHO85_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCQ2671511.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            32614..32958
                     /locus_tag="OHO85_002142"
     CDS             32614..32958
                     /locus_tag="OHO85_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCQ2671512.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(32995..33444)
                     /gene="alaE"
                     /locus_tag="OHO85_002143"
     CDS             complement(32995..33444)
                     /gene="alaE"
                     /locus_tag="OHO85_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCQ2671513.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         8286 a         8486 c         8949 g         7864 t
ORIGIN      
        1 gacaatatct atagtcaaat tgacagtgag gcaaagatga gtttttccgt tgatgtgctg
       61 gcgaatatcg ccatcgaatt gcagcgtggg attggtcatc aggatcgttt tcagcgcctg
      121 atcaccacgc tacgtcaggt gctggagtgc gatgcgtctg cgttgctacg ttacgattcg
      181 cggcagttta ttccgcttgc catcgacggt ctggcaaagg atgtactcgg tagacgcttt
      241 gcgctggaag ggcatccacg gctggaagcg attgcccgcg ccggggatgt ggtgcgcttt
      301 cccgcagaca gcgaattgcc cgatccctat gacggtttga ttcctgggca ggagagtctg
      361 aaggttcacg cctgcgttgg tctgccattg tttgccgggc aaaacctgat cggcgcactg
      421 acgctcgacg ggatgcagcc cgatcagttc gatgttttca gcgacgaaga gctacggctg
      481 attgctgcgc tggcggcggg agcgttaagc aatgcgttgc tgattgaaca actggaaagc
      541 cagaatatga tgccaggcga tgccacgccg tttgaagcgg tgaaacagac gcagatgatt
      601 ggcttgtccc ctggcatgac gcaactgaaa aaagagattg agattgtggc ggcgtccgat
      661 ctcaacgtcc tgatcagcgg tgagacggga accggtaagg agctggtggc gaaagcgatt
      721 catgaagcct cgccacgggc ggtgaatccg ctggtctatc tcaactgtgc cgcactgccg
      781 gaaagtgtgg cggaaagtga actgttcggg catgtgaaag gagcgtttac tggcgctatc
      841 agtaaccgca gcgggaagtt tgaaatggcg gataacggca ctctgtttct ggatgagatc
      901 ggcgagttgt cgttggcatt gcaggccaag ctgctgaggg tgttgcagta tggcgatatt
      961 cagcgcgttg gcgatgaccg cagtttgcgg gtcgatgtgc gcgtgctggc ggcgactaac
     1021 cgcgacttac gcgaagaggt gctggcaggg cgatttcgcg ctgacttgtt tcatcgcctg
     1081 agcgtgtttc cactttcggt gccgccgctg cgtgagcggg gcgatgatgt cattctgctg
     1141 gcggggtatt tctgcgagca gtgtcgtttg cggctggggc tctcccgcgt ggtattaagt
     1201 gccggagcgc gaaatttact gcaacactat cgttttccgg ggaacgtgcg cgaactggaa
     1261 catgctattc atcgggcggt agtgctgtcg agagccaccc gcaacggcga tgaagtgatt
     1321 cttgaggcgc aacattttgc ttttcctgag gtgacgttgc cgacgccaga agtggcggcg
     1381 gtgcccgttg ttaagcaaaa cctgcgtgaa gcgacagaag cgttccagcg tgaaactatt
     1441 cgtcaggcgc tggcacaaaa tcatcacaac tgggctgcct gcgcgcggat gctggaaacc
     1501 gacgtcgcca acctgcatcg gctggcgaaa cgtctgggaa tgaaggatta aataatcccg
     1561 gcctgataga aatcctgcag gttaattgcg ccggtgagtt tgccgttttc atctaccacc
     1621 ggtgcggcag tgattttgcg cttcatcagg atctctttgg cgtcgatggc gcgactttgc
     1681 gattgcaacg tggtgccgcc gaccgtcatc gcttcattaa ctggcgtggt gagtgcgccg
     1741 ccgccaacca gccagcgacg taaatcgccg tcggtaaaga cgccttgtac ctgttgttga
     1801 gcgtcacata ccgccaccag ccccagaccg gtgcggctga gttccagcat cgcatccatc
     1861 acgctggcgg ttaacgccac ctgtgggatg gcatcgtcac ggcgcatcag atgatgcact
     1921 ttattcagca agcgagcgcc cagtgccccg gctgggtggg agcgggcaaa atcttcttca
     1981 ttaaatccgc gcgcctgcat gaccgccatc gccagcgcgt cacccatcat cagggtattg
     2041 acggtgctgg aggtcggcgc aaggtgcatc gggcaggctt cgcgttctac ggagatatcc
     2101 agcaccgctt tcgccgccag gcccagcggt gacgtcggtt tgccggtcat cgccagcagc
     2161 gcgatagatt tatcttccag acgcggaata atcagatcca gttccttcgc gccaccggag
     2221 taagagataa acagcatcac atcgcggctt tcaatcatcc ccagatcgcc gtgcagcgct
     2281 tctgccggat ggacaaaaaa agccggtgtg ccggtactgg caagtgttgc ggcgattttc
     2341 ttaccaatgt ggcccgattt gccaattccc gaaaccacca ctttgccttc gcagtgcagg
     2401 atgatattgg cggcgcgaac aaaatcatcg cccagacgtt ccggtaaacg gcttgcttcc
     2461 tgcaactcca gcattaacgt ctgacgtccc gcgttcagta gtgcttcact cattgctctc
     2521 tccggttatg atcacatcga tccctttctc ttccagcgcc tgacgaaacg ccggatcgat
     2581 acctgcgtcg gtaatcagct tatcgacgct ttcaagactg caaactacgt tggggctttt
     2641 acggccaaac tttgatgagt ccgccatcaa aatcacttcg cgcgcggcat tgcacattgc
     2701 cttactgacg gtataaacct cgttgaaggt ggttacgccc gcattgagat cgatgccgtc
     2761 ggtgcccata aacaatttat cgaaactgaa atgttcgaag gcattctctg ccagctgtcc
     2821 gtgaaatgag gccgattttt tacgaaacgt tccgcctggc atcaggatag tttgttcgtt
     2881 atccagttcg gatagcgcat tgacgatatg caggctgttg gtcatcaccg tgatgttatt
     2941 aaagcgcgag agcaggggaa ccatctgcaa aacggtactg ccagcatcaa gaatgatcga
     3001 atcgccatca tggataaaac taacggcagc ttctgcaatc agctctttct tgtgggtgtt
     3061 gatgagcgtt ttatgatcga taggcggatc ggattcctct ttattcaaca ccactccgcc
     3121 ataagtacga atgacggttc cggcatgttc cagaatgacc agatctttgc gaatggttgt
     3181 gcctgtggtg tcaaagtatt gcgccaattc ttcaaccgag catttaccct gcttttgcag
     3241 atactccaga atggcggcct gacgctgacg aggtttcatg ggcgtgatta cctgatttag
     3301 gtttcaaaat gataacttcg caagcaagta acattcacaa cgaaattatc cacgtttcag
     3361 tttaagcgcc aatgatagag cattctgtga tagtggatca tggggaaaga tcacatcggg
     3421 ctgtaaatca cccgcatgca taccggtaat tgcgggaatt ttttgcggtc gggcgaagac
     3481 ggtcagtcct ttcataaaca aggtgtcgac gatgcgctgc tgatcgtcca gcgcgatggc
     3541 gaccacgacc cgcggtttaa agcgcccgct ggaacggccc acgccaaccc gcccttgctg
     3601 gcatagtgtg tcgaaggcac ggttaaaacg agaaatttgc cagccgccta aggccagttg
     3661 cgcacaccag gcgataacgg cgacggtgat gagtgcggat accataactt ttccttaatg
     3721 tagtggcgga agtcctgtat gcctgatgag cgtagcgcat caggcattct atagtctcac
     3781 ggagatcctt aacggatctc cgctgttaat cagaacatca cctgaccgcc ggtgacattg
     3841 atcgactgtc cggtgcagta cgacgcctta ggactggcgt agaacagcag catattcagc
     3901 acgtcctgat aatcgcagcc gcgtttgagc ggtactttgt cgatgtaata ctgctcgact
     3961 tgatccggtt tgatacccag cttggtcgcg tattgtggca acagtgactg gaacatcggc
     4021 gatttcagca ggttaccgag catcagtgaa tgcaccgtaa tgccgtactc cgccagatcc
     4081 agcgccagtg attgagtcag cccgacgcca ccaaatttcg ctgcgctgta gccagagttg
     4141 tgtttgctgc ccactttgcc ggatttcgag ttgatctgaa taatgcgacc ctgaatcccg
     4201 tcgcggatca tcaaacgcga aaattcacgg gcacacagga aataacccac cagattcact
     4261 tgtagcgaac ggtcaaaatc gccgagctgg aagtcgctga taaaggctgc ttttgctatt
     4321 ccggcgctat agaccagcaa atccacgcga ccaaagattt catctacccc acgagagagc
     4381 gccagaacgc tttgctcgct agtggcgtca gcaccaaaac cgtacgccat actttcacca
     4441 tattcggcgt taatttcttg tgccacattt gcggctttgt cgctctgaat atcgacaacc
     4501 gcgacgcgat acccctcggc agccagaccg tggcacagga acgcgcctaa tgtttgccca
     4561 ccaccgatga caacggcaac ctgattcatt ttttactcct taacagattc aaacttcaaa
     4621 accgatcccg gcgcgatatc gtcgggtaca gggcctgcca catggacagt gcccggaaat
     4681 tccgcttcgt ttaaaccatc gaagcgcagg gtgacatgac ccagttcgcg aaggttgtct
     4741 tccgccacgc tgccaacggc ggtcaccgga tagcgatgct gcccgagcgt aaattgcaaa
     4801 ccgggatgga gtgcaccttt caactcgccg tggcaatgaa tgaagcaata ctcttcgagg
     4861 tccgctggcg cgccttcacg aaaggtgatg agcatctggt cgctgagggc gtcagtggca
     4921 ctcgcgccga tacgggtgat ggtggtctga taaataacgg tcatgtttca gcctctattg
     4981 atagataaaa ccggagacaa accaggcgat cagtacagtc ggtgcgccgg ttaaaaagcg
     5041 gctcaccagt acagaaggga caccgacgcg aaccgtgtcc tgacgggctt ccgccagcga
     5101 caaaccgacc gggatgaagt cgcaggccgc ctgcgcgttg atggcaaaca gtgccggtaa
     5161 agccagatgc ggcggaatat tgccgagacc aatctgcacg ccaatcaata cgccgataac
     5221 ctgtgcgata actgcgcctg ggccgaggaa aggtgaaagc agtgggaagg agcagatgag
     5281 cgccagcatg accagaccca gtggatggct cgccagcgga gcaagaccgt gggcaatcca
     5341 gtcaccaagg ccagaagcca taatgatgcc aatgagcgcc gagacgaatg ccataaacgg
     5401 cagaatggtt tttaatacag tgtcgatggt gtcacgacca gattgaaaca acacggcaac
     5461 ggcagaaccc atccccattc ccaccttcgc cagtaaacca tcgctttgtt cggtgatttt
     5521 cttgctggtg tcatagtcac ggcccacgga agagggttgt ggtgtcgcat cacccactac
     5581 agtgatgttt tcttctttta cgccagagac ataaatatct tccacaatgt actgcgccag
     5641 tggaccggac ttgcccgtcg agtggatatt aatggtggga atacgccgtt tcggatagat
     5701 gccgcagcgt aatgtgccgc cacagtcgat taccgcgaca ccaatttccg cctccgcggg
     5761 ttcaccttct ttaaatccgt caatagcctg ccagccagta agttgtgcca gtttgtcaac
     5821 aatcgcaggc cgggtaccgg cggtgatata gacgattttt ttgcccggcg tggcttccag
     5881 ctcaagcggg ccgccccagc cacccgttcc tttttcgatc cgaatacgcg tcatgatgtt
     5941 gctcctgcca ggtgaacttt ttgttcaagt tgaatgccca tctttttctc aaaaacggcg
     6001 gtggtcagat cggtcaccca gccgcggaag aaattggtga ccagaccaac cagcagataa
     6061 ctcaccgcca gtgggccaag tggcaggttc agcgttgtca gaccgctggc aatgccaaga
     6121 taaacaaaca gttcgccagg gttgatatgg gggaagaggc cattcattga gtggcagcta
     6181 taagaggccg ccgcgtagta gctgggtttg tacttttccg gcataaagcg acccaggctt
     6241 aaggtcatcg gattgcaaaa gacaaacgtg ccaatgcacg gtaacagtag gtaacgggaa
     6301 acagggttac cggcgcaacg ttgagcaaaa cgttcaatac gatgctgacc gataaaatta
     6361 atcagtgcgt tcatgataac cagcaggcta atcaacagcg gaagaatgcc ggtcaccatc
     6421 ccggtaaaca cctctccgcc cttttggaac agcccgataa accactctgc accatgagta
     6481 atggtttcta tcattgttct ctccttcagg atttattgtt ttattaccaa acggcaacct
     6541 aatctaatca gattgaaaga tttaaaagtg ttattttgat ctcaaaatga aagataaata
     6601 ttttaatttg aaagtttgag taaaaggata gcgaggggaa tgagttgagt tatgtaaagt
     6661 ccgtatcggg cagtgactac cgcttccttg tgtggggcgt gatgctttac catacttgcc
     6721 cctggttgaa tctgttaaat ggacccctca tgttcaagcg tcgttatgta acattgcttc
     6781 ccctttttgt gttgcttgcc gcctgtagca gcaagccaaa acctactgag actgaaacga
     6841 ccaccggaac gccgtctggc ggcttcctgc ttgagccgca gcacaatgtg atgcagatgg
     6901 gcggcgattt cgctaataac ccgaatgccc agcagttcat cgacaaaatg gtgaacaaac
     6961 acggtttcga tcgtcagcag ttgcaggaaa ttctctccca ggcgaagcgt ctggattcgg
     7021 tactgcggct gatggataac caggcaccaa ccacatcggt gaaaccgcca tcaggtccga
     7081 acggcgcatg gctccgttat cgcaaaaaat ttattacgcc ggacaacgtg cagaacggtg
     7141 tggttttctg gaatcagtat gaagatgcgt tgaatcgcgc gtggcaggtg tatggcgtac
     7201 cgccggaaat tatcgtcggg attatcggcg ttgaaacccg ctgggggcgc gtgatgggga
     7261 aaactcgcat cctcgatgcg ctggcaacgc tgtcatttaa ctacccacgc cgcgcggagt
     7321 atttctctgg cgagctggaa accttcctgt tgatggcgcg cgacgagcag gacgatccgc
     7381 tcaatctgaa aggttccttt gccggggcga tgggctacgg acagtttatg ccgtcgtctt
     7441 acaaacaata tgcggtagat ttcagcggcg acgggcatat caacctgtgg gatccggttg
     7501 atgcgatcgg tagcgtggcg aactatttca aagcgcacgg ctgggtgaaa ggcgatcagg
     7561 tcgcggtaat ggcaaacggt caggctccag gcttgccaaa tggcttcaaa accaggtaca
     7621 gcatttcgca gcttgccacg gcaggtttaa cgccacagca gccgctgggc aaccatcaac
     7681 aagccagcct gctgcgtctg gatgtcggta ccggttacca gtactggtac ggtctgccga
     7741 acttctacac cattacccgt tataaccaca gcacccatta cgcaatggcg gtctggcagt
     7801 taggacaagc cgtggcgctg gcgcgcgtgc agtaactatt tgcaagaggg gggcgtgagc
     7861 ttccctctgg ctaatcagct tttttctgaa tcctcctcgt aaaattgcaa cgccaacacc
     7921 atcttcctga cgaaagtgct atcttgtccg gcataaattt ttgactgaca gaggttgtga
     7981 tgactgacag tgaactgatg cagttaagtg aacaggttgg gcaggcgctg aaagcccgtg
     8041 gcgcaaccgt aacaactgcc gagtcttgta ccggtggttg ggtagcgaaa gtgattaccg
     8101 atattgccgg tagctccgcc tggtttgaac gcggatttgt cacctacagt aacgaagcca
     8161 aagcgcagat gatcggcgta cgcgaagaga cgctggcgca gcatggcgcg gtgagtgaac
     8221 ccgtcgtggt ggaaatggcg ataggtgcac tgaaagcggc tcgtgctgat tatgccgtgt
     8281 ctattagtgg tatcgccggg ccggatggcg gcagtgaaga gaagcctgtc ggcaccgtct
     8341 ggtttgcttt tgccactgcc cgcggtgaag gcattacccg gcgggaatgc ttcagcggtg
     8401 accgtgatgc ggtgcgtcgt caggctactg cgtatgcatt gcagaccttg tggcaacaat
     8461 ttctacaaaa cacttgatac tgtatgagta tacagtataa ttgcttcaac agaacatatt
     8521 gactatccgg tattacccgg catgacagga gtaaaaatgg ctatcgacga aaacaaacag
     8581 aaagcgttgg cggcagcact gggccagatt gagaaacaat ttggtaaagg ctccatcatg
     8641 cgcctgggtg aagaccgttc catggatgtg gaaaccatct ctaccggttc gctttcactg
     8701 gatatcgcgc ttggggcagg tggtctgccg atgggccgta tcgtcgaaat ctacggaccg
     8761 gaatcttccg gtaaaaccac gctgacgctg caggtgatcg ccgcagcgca gcgtgaaggt
     8821 aaaacctgtg cgtttatcga tgctgaacac gcgctggacc caatctacgc acgtaaactg
     8881 ggcgtcgata tcgataacct gctgtgctcc cagccggaca ccggcgagca ggcactggaa
     8941 atctgtgacg ccctggcgcg ttctggcgca gtagacgtta tcgtcgttga ctccgtggcg
     9001 gcactgacgc cgaaagcgga aatcgaaggc gaaatcggcg actctcacat gggccttgcg
     9061 gcacgtatga tgagccaggc gatgcgtaag ctggcgggta acctgaagca gtccaacacg
     9121 ctgctgatct tcatcaacca gatccgtatg aaaattggtg tgatgttcgg taacccggaa
     9181 accactaccg gtggtaacgc gctgaaattc tacgcctctg ttcgtctcga catccgtcgt
     9241 atcggcgcgg tgaaagaggg cgaaaacgtg gtgggtagcg aaacccgcgt gaaagtggtg
     9301 aagaacaaaa tcgctgcacc gtttaaacag gctgaatttc agatcctcta cggcgaaggt
     9361 atcaacttct acggcgaact ggttgacctg ggcgtaaaag agaagctgat cgagaaagca
     9421 ggcgcgtggt acagctacaa aggtgagaag atcggtcagg gtaaagcgaa tgcaactgcc
     9481 tggctgaaag ataacccgga aaccgcgaaa gagatcgaga agaaagtacg tgagttgctg
     9541 ctgagcaacc cgaactcaac gccggatttc tctgtagatg acagcgaagg cgtagcagaa
     9601 actaacgaag atttttaatc gttcgttatt gatgataata gataatgtgt gcacggacgg
     9661 tcccctcgcc cctttgggga gagggttagg gtgaggggaa caggccagca ctggcgtgaa
     9721 aaattactct cacctcgatc ctctaacgaa ttgggcctgc gggaacattc tgcgcactca
     9781 ttgactatta aatctgttta tcagcaaaac ctgggtcgca tctgcggccc ttttgctttt
     9841 ttaagttgta aggatatgcc atgacagaat caacgtcccg tcgcccggca tatgctcgcc
     9901 tgttggatcg tgcggtacgc attctggcgg tgcgcgatca cagtgagcaa gaactgcgac
     9961 gtaaactcgt ggcaccgatt atgggcaaaa atggcccaga agagattgat gctacggcag
    10021 aagattacga gcgcgttatt gcctggtgcc atgaacatgg ctatctcgat gacagccgat
    10081 ttgttgcgcg ctttatcgcc agccgtagcc gcaaaggtta tggacctgca cgtattcgtc
    10141 aggaactgaa tcagaaaggt atttcccgcg aagcgacaga aaaagcgatg cgtgaatgtg
    10201 acatcgactg gtgcgcactg gcgcgcgatc aggcgacgcg aaaatatggt gaacctttgc
    10261 caactgtctt ttcagaaaaa gttaagatcc agcgttttct gctctatcgt ggctatctga
    10321 tggaagatat ccaggatatt tggcgaaatt ttgccgactg aacgcatacg gtattttact
    10381 tcccagtcaa gaaaacttat cttattccca cttttcagtt accagcccgg cggttaagac
    10441 acgctggagc tggtggcgat atttcgttag cttgatttca ggataattat gagcaagagc
    10501 accgctgaga tccgtcaggc gtttctcgac tttttccata gtaagggaca tcaggtagtt
    10561 gccagcagct ccctggtacc ccataacgac ccaactttgt tgtttaccaa cgccgggatg
    10621 aaccagttca aggatgtgtt ccttgggctc gacaagcgta attattcccg cgctaccact
    10681 tcccaacgct gcgtgcgtgc gggtggtaaa cacaacgacc tggaaaacgt cggttacacc
    10741 gcgcgtcacc ataccttctt cgaaatgctg ggcaacttca gcttcggcga ctatttcaaa
    10801 cacgatgcca ttcagtttgc atgggaactg ctgaccagcg aaaaatggtt tgccctgccg
    10861 aaagagcgtc tgtgggttac cgtctatgaa agcgacgacg aagcctacga aatctgggaa
    10921 aaagaagtcg ggatcccgcg cgaacgtatt attcgcatcg gcgataacaa aggtgcgcca
    10981 tacgcatctg acaacttctg gcagatgggt gacactggtc cgtgcggccc gtgcaccgaa
    11041 atcttctacg atcacggcga ccacatttgg ggtggccctc cgggaagtcc ggaagaagac
    11101 ggcgaccgct acattgagat ctggaacatc gtcttcatgc agttcaaccg ccaggcagat
    11161 ggcacgatgg aaccgctgcc gaagccgtct gtagataccg gtatgggtct ggagcgtatt
    11221 gctgcggtgc tgcaacacgt taactctaac tatgacatcg acctgttccg cacgttgatc
    11281 caggcggtag cgaaagtcac tggcgcgacc gatctgagca ataaatcgct gcgcgtaatc
    11341 gctgaccaca ttcgttcttg tgcgttcctg atcgcggatg gcgtaatgcc gtccaatgaa
    11401 aaccgtggtt atgtactgcg tcgtatcatt cgtcgcgcag tgcgtcacgg caatatgctc
    11461 ggcgcgaaag aaaccttctt ctacaaactg gttggtccgc tgatcgacgt tatgggctct
    11521 gcgggtgaag acctgaaacg ccagcaggcg caggttgagc aggtgctgaa gactgaagaa
    11581 gagcagtttg cccgtactct ggagcgcggt ctggcgttgc tggatgaaga gctggcaaaa
    11641 ctttctggtg atacgctgga tggtgaaacg gctttccgtc tgtacgacac ctatggcttc
    11701 ccggttgacc tgacggctga tgtttgtcgt gagcgcaaca tcaaagttga cgaagctggt
    11761 tttgaagcag caatggaaga gcagcgtcgt cgcgcgcgcg aagccagcgg ctttggtgcc
    11821 gattacaacg caatgatccg tgttgatagt gcatctgaat ttaaaggcta tgaccatctg
    11881 gaactgaacg gcaaagtgac cgcgctgttt gttgatggta aagcggttga tgccatcaat
    11941 gcaggccagg aagctgtggt cgtgctggat caaacaccat tctatgcgga atccggcggt
    12001 caggttggtg ataaaggcga actgaaaggc gctaacttct ccttcgcggt ggaagatacg
    12061 cagaaatacg gccaggcgat tggtcacatc ggtaaacttg ctgcgggttc tctgaaagtg
    12121 ggcgacgctg tgcaggctga tgttgatgag gctcgtcgcg cccgtattcg tctgaatcac
    12181 tccgcaacgc acctgatgca cgctgcgctg cgccaggttc tggggactca tgtatcgcag
    12241 aaaggttcac tggttaacga caaggtgctg cgcttcgact tctcacacaa cgaagcgatg
    12301 aaaccggaag agattcgtgc ggtcgaagac ctggtgaacg cacagattcg tcgcaatttg
    12361 ccgatcgaaa ccaacatcat ggatctcgaa gcggcgaaag cgaaaggtgc gatggcgctg
    12421 ttcggcgaga agtatgatga gcgtgtacgc gtgctgagca tgggcgattt ctccaccgag
    12481 ctgtgtggcg gtactcacgc cagccgcact ggtgatattg gtctgttccg catcatctct
    12541 gaatcgggta ctgctgcagg cgttcgtcgt atcgaagcgg taaccggaga aggcgctatc
    12601 accaccgttc atgcagacag cgatcgctta agcgaagtcg cgcatctgct gaaaggcgat
    12661 agcaataatc tggcggataa agtgcgctca gtactggaac gtacgcgtca gttagaaaaa
    12721 gaactacaac agcttaaaga acaagctgcc gcacaggaga gcgcaaatct ttccagtaag
    12781 gcaattgatg ttaatggtgt taagctgttg gttagcgagc ttagcggtgt tgagccgaaa
    12841 atgttgcgta ccatggttga cgatttaaaa aatcagctgg ggtcgacaat tatcgtgctg
    12901 gcaacggtag ccgaaggtaa ggtttctctg attgcaggcg tatctaagga cgtcacagat
    12961 cgtgtgaaag caggggaact gattggtatg gtcgctcagc aggtgggcgg caagggtggt
    13021 ggacgtcctg acatggcgca agccggtggt acggatgctg cggccttacc tgcagcgtta
    13081 gccagtgtga aaggctgggt cagcgcgaaa ttgcaataat ataagcgtca ggcaatgccg
    13141 tggactcgct tcacggcatt cgcattaacg ctatcgacaa cgataaagtc aggttgaagt
    13201 tgtgtatatc ggctaaactt aggtttaaca gaatgtaatg ccatgactgc ttacatgtaa
    13261 tgtgtttgtc attgcttact ttttggcgtt atatgatgga taatgccggg atacagagag
    13321 acccgactct tttaatcttt caaggagcaa agaatgctga ttctgactcg tcgagttggt
    13381 gagaccctca tgattgggga tgaggtcacc gtgacagttt taggggtaaa gggcaaccag
    13441 gtacgtattg gcgtaaatgc cccgaaggaa gtttctgttc accgtgaaga gatctaccag
    13501 cgtatccagg ctgaaaaatc ccagcagtcc agttactaat ctttccgcgt ctcatcttta
    13561 tcggtgagac gcaccctcaa aatttctcct tcactctata gtcttttcgc tttactcccg
    13621 ttcattcaac ttaagtctcc atttttttgc attactacta tctgtcagac ctccattttt
    13681 ctgttgataa aacactcttt ttgacgtttt tacagactaa ttgaacgtga agtgtgcaaa
    13741 cgataaaagt gtaggaaaaa ttgtttgact tataagtctc agaaagtaat atgtgcgcca
    13801 cgcagcgacg atgagcgata aacaagttct tcgaagcact cgtaagaggc gtgtggtgag
    13861 gtggccgaga ggctgaaggc gctcccctgc taagggagta tgcggtcaaa agctgcatcc
    13921 ggggttcgaa tccccgcctc accgccattt gcatccgtag ctcagctgga tagagtactc
    13981 ggctacgaac cgagcggtcg gaggttcgaa tcctcccgga tgcaccatat tctacgtact
    14041 ttcagcgatg aaggtatgga agaggtggcg gtataaccgc aggcaccagg gaggataacg
    14101 ttgctttagc aacggcccga agggcgagcc gcaaggcgag taatcctccc ggatgcacca
    14161 tctcttactt gatacggctt tagtagcggt atcaaagaat ctgcagtaaa gtaagtttcc
    14221 cgatgcatcc gtagctcagc tggatagagt actcggctac gaaccgagcg gtcggaggtt
    14281 cgaatcctcc cggatgcacc atctcttact tgatatggct ttagtagcgg tatcaatatc
    14341 agcagtaaaa taaattttcc cgatgcatcc gtagctcagc tggatagagt actcggctac
    14401 gaaccgagcg gtcggaggtt cgaatcctcc cggatgcacc atattctccg aaatcttcag
    14461 caatgaaggt atcgaagagg tagcggaatt aaccgcaggc accagggatg ataacgttgc
    14521 tttagcaacg gcccgaaggg cgagccgcaa ggcgagtaat cctcccggat gcaccatctc
    14581 ttacttgata tggctttagt agcgatatca atatcagcag taaaataaat tttcccgatg
    14641 catccgtagc tcagctggat agagtactcg gctacgaacc gagcggtcgg aggttcgaat
    14701 cctcccggat gcaccatatt ctccgtaacc ttcagcaatg aaggtatcga agagatagcg
    14761 gtattaaccg caggcaccag ggaggataac gttgcgtcag caacggcccg aagggcgaac
    14821 gccagtgagt aatcctcccg gatgcaccat cttctcccaa aaatttcctt caaaaatatc
    14881 tcatttctaa ataactactt tatccatttc ctgaattttc ttctccttcg ccatcagcga
    14941 caaaatcaag caattacgct aaagtagcgt catatttttc gacttgcgag aagaccatgt
    15001 acgagcgtta tgcaggttta atttttgata tggatggcac aatcctggat acggagccta
    15061 cgcaccgtaa agcgtggcgc gaagtattag ggcactacgg tcttcagtac gatgttcagg
    15121 caatgattgc gcttaatgga tcgcccacct ggcgtattgc tcaggcaatt attgagctga
    15181 atcaggccga tctcgacccg catgcgttag cgcgtgaaaa aacagaagca gtaagaagta
    15241 tgctgctgga tagtgtcgag ccgcttcctc ttgttgaagt ggggaaaagt tggcatggtc
    15301 gtcgcccaat ggctgtagga acggggagtg aaagcgccat cgctgaggca ttgctggcgc
    15361 acctgggatt acgccgttat tttgacgccg tcgtcgctgc tgatcacgtc aaacaccata
    15421 aacccgcgcc agacacattt ttgttgtgcg cgcagcgtat gggcgtgcaa ccgacgcagt
    15481 gtgtggtctt tgaagatgcc gatttcggta ttcaggcggc ccgtgcagca ggcatggacg
    15541 ccgtggatgt tcgcttgctg tgagtgaagc gttatcgctt ttctcgttgt ttgccagtag
    15601 ttttctcagc gctacactat tacccggcaa ctccgaagtc gtgctggtgg caatgttgct
    15661 ttccgggatc agttatccct gggttttagt tttaacagca acaatgggta atagccttgg
    15721 agggttaact aacgttatcc ttgggcgttt ctttccattg cgtaaaacat cgcgctggca
    15781 agagaaagct accggctggc tgaaacgcta tggtgcagtc acgctattat taagctggat
    15841 gcccgtggtt ggcgatttac tgtgtctgtt agcgggatgg atgcgcatct cctgggggcc
    15901 agtaatcttt tttttgtgcc ttggtaaagc gttacgctat gttgcagttg cagcagcgac
    15961 cgttcagggc atgatgtggt ggcactaatt gtaggcctgc acatatggtc accattacag
    16021 ttatgctaat taaaacgatt ttgacaggcg ggaggtcaat ttgatcccgg acgtatcaca
    16081 ggcgctggcc tggctggaaa aacatcctca ggcgttaaag gggatacagc gtgggctgga
    16141 gcgcgaaact ttgcgtgtta atgctgatgg cacactggca acaacaggtc atcctgaagc
    16201 attaggttcc gcactgacgc acaaatggat tactaccgat tttgcggaag cattgctgga
    16261 attcattaca ccagtggatg gtgatattga acatatgctg acctttatgc gcgatctgca
    16321 tcgttatacg gcgcgcaata tgggtgatga gcggatgtgg ccgttaagta tgccatgcta
    16381 catcgcagaa ggtcaggaca tcgaactggc acagtacggc acttctaaca ccggacgctt
    16441 taaaacgctg tatcgtgaag ggctgaaaaa tcgctacggc gcgctgatgc aaaccatttc
    16501 cggcgtgcac tacaatttct ctttgccaat ggcattctgg caagcgaagt gcggtgatat
    16561 ctcgggcgct gatgccaaag agaaaatttc tgcgggctat ttccgcgtta tccgcaatta
    16621 ctatcgtttc ggttgggtca ttccttatct gtttggtgca tctccggcga tttgttcttc
    16681 tttcctgcaa ggaaaaccaa cgtcgctgcc gtttgagaaa accgagtgcg gtatgtatta
    16741 cctgccgtat gcgacctctc ttcgtttgag cgatctcggc tataccaata aatcgcaaag
    16801 caatcttggt attaccttca acgatcttta cgagtacgta gcgggcctta aacaggcaat
    16861 caaaacgcca tcggaagagt acgcgaagat tggtattgag aaagacggta agaggctgca
    16921 aatcaatagc aacgtgctgc agattgaaaa cgaactgtat gcgccgattc gtccaaaacg
    16981 cgttacccgc agcggcgagt caccttctga tgcgctgtta cgtggcggca ttgaatatat
    17041 tgaagtgcgt tcgctggaca tcaacccgtt ctcgccgatt ggtgtagatg aacagcaggt
    17101 gcgattcctc gacctgttta tggtctggtg tgcgctggct gatgcaccgg aaatgagcag
    17161 tagcgaactt gcctgtacgc gcgttaactg gaaccgggtg atccttgaag gtcgcaaacc
    17221 gggcctgacg ctgggtatcg gctgcgaaac cgcgcagttc ccgttactgc aggtgggtaa
    17281 agatctgttc cgcgatctga aacgcgtcgc gcaaacgctg gatagcatta acggcggcga
    17341 agcgtatcag aaagtgtgtg atgaactggt tgcctgcttc gataatcccg atctgacttt
    17401 ctctgcccgt atcttaaggt ctatgattga tactggtatt ggcggaacag gcaaagcgtt
    17461 tgcagaagcc taccgtaatc tgctgcgtga agagccgctg gaaattctgc gcgaagagga
    17521 ttttgtagcc gagcgcgagg catctgaacg ccgtcagcag gaaatggaaa ccgctgatac
    17581 cgaaccgttt gcggtgtggc tggaaaaaca cgcctgacag aaaagaaaaa ggccactcgt
    17641 gagtggccaa aatttcatct ctgaattcag ggatgatgat aacaaatgcg cgtctttcat
    17701 atactcagac tcgcctggga agaaagagtt cagaaaattt taaaaaaaat taccggaggt
    17761 ggctaaatgc cgttgttaga tagcttcaca gtcgatcata cccggatgga agcgcctgca
    17821 gttcgggtgg cgaaaacaat gaacaccccg catggcgacg caatcaccgt gttcgatctg
    17881 cgcttctgcg tgccgaacaa agaagtgatg ccagaaagag ggatccatac cctggagcac
    17941 ctgtttgctg gttttatgcg taaccatctt aacggtaagg gtgtagagat tatcgatatc
    18001 tcgccaatgg gctgccgcac cggtttttat atgagtctga ttggtacgcc agatgagcag
    18061 cgtgttgctg atgcctggaa agcggcaatg gaagacgtgc tgaaagtgca ggatcagaat
    18121 cagatcccgg aactgaatgt ctaccagtgt ggcacttacc agatgcactc gttgcaggaa
    18181 gcgcaggata ttgcgcgtag cattctggaa cgtgacgtac gcatcaacag caacgaagaa
    18241 ctggcactgc cgaaagagaa gttgcaggaa ctgcacatct agtcagtaaa ctatcttcac
    18301 aattaattga aaaaagccag ttcaaatgaa ctggcttagt tgtacttagt gcgcaccgcc
    18361 tccgccgcca cctgcgccaa atggcggttt agcaaaccac accagcccca gcagtacgag
    18421 gaatatcccg gctgacatcc agaagatctc attggcggaa ataatcagcc cctgattggt
    18481 gatctgctgg gcaatccagc ctgatgcctg ctgttgcgtc atcccaagcc cttccagttg
    18541 actgtacatc gcctgggcat tcgggttgaa cgggtttacc gactcagtca actgcgcatg
    18601 gtgcatcgac tcgcggttgg tccacatggt cgtggttatc gacgtgccga tagaccccgc
    18661 cagcgttcgc gtaaagttag agaggctcga tgccgccgcc agtcgttccg gtggcaaacc
    18721 agacagcgta atggtggtca gcggcataaa gaagcaggcc accgcaaacc cctggataaa
    18781 ctgcggccag gccgacgcgc caaaatccat acctggttca aaggtatagg cacgccagta
    18841 gaagcagacg gcatacataa taaagctgaa ggttaccagc cgccgcatat ccagtttatg
    18901 cgcgaagcgg ccgataatcg gcgacaggat caccggaata atccctaccg gcgcagaggc
    18961 caaacctgcc caggtcgccg tgtaaccgta gacctcctgc aacaactgcg gcagcagaac
    19021 aatagcgccg aagtagagca tatacgcgag gctgatacac aagcagccga tggtgaagtt
    19081 gcgcgactta aacaacgaca gatcgactat cgggttatcg tcggtcagct cccagacaat
    19141 caggaagcag atagccacca ccgccaccac ggtaagaatg ataatttcct gtgatgaaaa
    19201 ccagtccagc tctttaccgc ggtcgagcat aatctgcagg ctgccgatac caataaccag
    19261 cagtgccagc cccacggcat caatccgccg ccgttcggtg cgggtttcac gtccgcgcag
    19321 agtttgcagt gtcatcaaca ccaccgccac gccaatcggc acgttgatga agaatatcca
    19381 gccccagtgg taattatcgc tgatataacc gccgaggatc gggccgcaaa ttggcgcgac
    19441 aatcaccgtc atcgaccaca acgccagcgc gatcgagcgt ttggctggcg ggtagttatt
    19501 cagcaataga ctttgcgaaa gcgggatcaa cggcccggcg acaatcccct gaatcacgcg
    19561 gaagaagatc agcatattca ggctgctgga gacaccacac gcccacgacg caatagcaaa
    19621 ggcgatggtg gaccaaagga acagtttcac ttccccgacg cgctttgcca gccagccggt
    19681 aagcgggatc gagatggcat tcgccacccc gaaagaagtg attacccacg ttccctggct
    19741 gagcgatgag cccagattcc cggcgatagt ggggatcgcc acgttagcaa tggtggagtc
    19801 cagcacctgc atgaatgtcg ccagtgacag cgcaatcgtc ataatgacca gttgcgcgcc
    19861 ttccagcggt ttttgctgtt gcatcacacg cacctctgga ttagccagcg ttagctttta
    19921 cgatatcgtc gatcagttta ttgacaggtg ccaggctgat ttcacgcgcg gtacttaccg
    19981 cgaccggtgt tgaacgtact ttatttgcca gtacctgacc gtcacggtta gtggtattga
    20041 cgctcaccag cgtggacaaa ccaatgcgca gcgggtattg ctccagctgt ttctggtcca
    20101 gttcgatacg cacaggtaga cgctgaacga ctttgatcca gttaccggtc gcattttgcg
    20161 ccggaagcag tgagaacgcg ctacctgtgc ccatatccag accaaccact ttaccggtgt
    20221 atttcacatc atcgccgtaa atatcggtgg tgatggtgac cggctgaccg atgcgcatat
    20281 tggcaatctg cgtctcttta aagttggcat ccacccacat attggtggct ggaacgaccg
    20341 ccatcagcgg cgtcgttggg ctaatttgcg cacccggttg cacggcacga cgggaaacat
    20401 aaccagtcat cggactgaca atacgggtac gctccagcgc cagccaggcg ttacgtactt
    20461 cggtggcagc ctgttgcacg gcaggctgat cttccagttt agtccccaga atcatcgcct
    20521 gattggcatt gtattgttga atagcgacgt ccagttgcgc ctgggcactg gtgacggcgt
    20581 cgcgggcgtg ttgcagctct tcgcgaccaa tcaggttggc attgcccagc ggcacacggc
    20641 ggttgtagtc gctttgtgct ttcgcgaggg cgattttctg cacctcaata ttcgcctgca
    20701 actgcttgct gttaatcatc agctggtggg tttggcgaac gctggaagcc agtgcagttt
    20761 tggctttttc aaacgcctgg cgagcatctg tcgggtcgag agtgaccaga acatcgcctt
    20821 cttttacaaa atcggtgtta tcggcccaga ctttcgtcac gctgccagac acctgagaca
    20881 taatttgaat ctgattccct gccacgtatg cgtcatcggt ttcttcgaaa tgacgcagta
    20941 ccaaaaacca ataaatccct atcgctacgg caataattat aaagagcaag gtgagaagga
    21001 ggagcagacg cttacgtttg ccgctcttct ttaccggttg ctgcggggtt tgagtctccg
    21061 catttgcgct catattgttc tccacgatct tcttaactca tcggctgagc cgacctgctt
    21121 gccaaaaagg ccagcatgtt gcgatgctgg ccagtcattt tttcttttat aaatctggat
    21181 ttttgagcga gatgacgcgt tagctcatcg cttcgagaac cacaccgtct tgttccatct
    21241 ggtcgagacg ggagagcaat ttgcgggtga tttgctcgag ctgatctttt tctgttgtgc
    21301 tgagcgcgga ccagagttga tgcaggcagt tatgctgcgg tggtaaaacc tcgcgcaaaa
    21361 actcgtgacc tttttccgtt aattgcagat gcaggcagcg gcgatcgtta tcgctttcac
    21421 gacgttcgat ccaaccgcgt ttttccagtt catcggcaat acgcgtcgcg ttggtacggg
    21481 atgatccaag agcacaactt aattcagaag gctgaatact gtggttttcc tgagactcca
    21541 gcgtaatcaa cgccataaac aacgtctcgt taatcccctg agccttcagc attttattgc
    21601 ggttctccag cagcttgctt tgcatgtgca tgcaaagacg agtcagaagg atctcctgat
    21661 aaggaaaatc ttcgtggcgg ctggcgcgaa attttagcat ttgttcaatg ggcgtaaacg
    21721 aactatccat ttgggtatga cctcattaat ttcagccgat atagtaacga cagtgacaaa
    21781 taaagtaaat gtattgtttt agaaaaatga ttcttgtgga ttatataatc gccatcactt
    21841 tccagacgag cccgtaggtc agcgcactaa gcagtgttgg gatgataatg ctgcgtgttt
    21901 tatagaaact ggcacccagt accgcgaagc cgaccagcgt gggcacgaaa cggcgtgtat
    21961 cgtgcatcac ttctggtgcg gtagagacaa ccagcagagc gcatatcgag gcgatgccaa
    22021 tggtgtcgag caaaatacct accgcgccac gtttggttgg gcgggcatta cccacacgca
    22081 ggcgcagcgg caaatagcgg aagcaataat tcgccgcgcc aaccagtaac ccaagcagca
    22141 gaacctcata gctcatcggg cgctccttgc cagaatgcct ggattaacgc agtgaggcag
    22201 ccacagacaa tgcctgccag aatggcgacg ggaatagaaa atagcgttac gcctgcaagg
    22261 gcaccaacta acgctgcggt aacgcaaaga gattgtttgc gctggaaaga ggcgagcagg
    22321 aaactcataa agagtgccgg aagcataaaa cctaatgctg cttcaacggc gggataacct
    22381 tgcagcaagc cgctgccgga gaatgcccct attaccgtac caaataccca cgatgaccat
    22441 gaactgaagg caatgccgat catccagttc tcgctccagc ggcgattatt gcgtaccagt
    22501 tttgcggttg cggcggcaaa aacctcatcc gtcaggccaa acgcccacag ggcggttttc
    22561 gatttttgca gacgctgaat aatacggcta cgcagtgacg ggccatacaa cacatggcga
    22621 acatccattg ccatgacggt cagtgcagca acccacaaac tactcccggc tgccagcatc
    22681 gcggtaatga cgaactggct cgcgcctgca taaatgatgc aggagaaaaa aacgctttcg
    22741 agaggagaga atcccagacg ggtcgcattc agaccgaacg caaaggccac cggaatataa
    22801 ctaataacaa tcggtaaact gtctttgcat ccttccatga aggtcgccga accaggagca
    22861 ggctgtggag tagggctttc catagagtgt acgcttaacg attgttaata ttataaatag
    22921 actgaatgaa tatcttaacc ttatcagact gatgggcttc ttaacaccct tataagtgta
    22981 aagccacgaa aacggttgct gattgcttaa tttgcggctt cctgacgatg aaaacctcgc
    23041 caccagacca gtaagttaac cagggcaatc gtcgcgccag ccagacaaac gccagcccaa
    23101 ccgccatgtt gccaggctga ggctgaaatt agcgaaccgg cggcaccgcc aataaagtag
    23161 ctagtcatgt aacctgcggt caggcgatta cgcgcatcag gatgtatccg ataaattacc
    23221 gtctggttag tgatatgcac gccctgcacg gtgagatcca gcaccaggat tccgataatc
    23281 aacgccagta cggaagtgtg accaaaccag atcgccagcc atgaaagtaa tagcagcagc
    23341 agaccgaaag ttgtggtgtg gtgcgatttg cccttatcgg caaaaccgcc cgccggacga
    23401 gcgcccaacg ctccggcagc tcccgcaagt ccaaacagac caattacacc atcgctgtag
    23461 ttaaaaggtg gagcggcaag caaaaaggcc attgaggtcc agagaatgct gaaattggca
    23521 aaggtcaggc agcccagcaa cgcgcgggta cgcagaattt tatcgctgat aaacatactg
    23581 aaaacggaac ccaacaactg tgggtagttg aggtgggttt ctgatttcat ttgtggcaga
    23641 ccacgccata atgccagcgc catcagtgcc attaacaccg aagcaaccca aaagacggtg
    23701 cgccagccgc cgagattcgc cagcaatccg gcaactgtcc gtgccagcaa gatccccaac
    23761 agcagaccgc tcataatagt gccaaccact ttgccgcgtt tgtccggtga agccagcgtc
    23821 gctgccagcg gaaccagaat ttgtgccacg acagagaata aaccggttaa tgccgtaccg
    23881 aggatcatca tcgccagcga ctgactgctg gcagtgatta acatgccgcc tgccgccagt
    23941 aaggtcatcg agacaatcag gcggcggcgt tcaaacatat caccgagggg aacaagaaac
    24001 agcagacctg ctgcatagcc caattgcgcg gcggtaacaa taaagcctgc cgaactggcg
    24061 gaaagggaaa agttacgcgc gatggtgtcg agcaatggct gagcgtaata gttactggcg
    24121 accgccagac cggtggcgat agacatcagc acgatcagcg ccgggctaag ctcatgatta
    24181 ggtttagtca ttatttttgt tcatcaggtg atgattaagc tggaaatcaa taatagcgga
    24241 tgttgatgaa tatgggatga aagatagctt gttgaaatgt gcgatgtggt ttgtaggcat
    24301 gataagacgc gccagcgtcg catcaggcat tgtgcacaga acggcggatg cggcgaaaac
    24361 gccttatccg ccatacggaa tgagtgtatt ttgtaggcat gataagacgc gtcagcgtcg
    24421 catcaggcat tgtgtacaga gcggcggatg aggcaaaaac gccttatccg cctgaataaa
    24481 aattacttct gcgctgccag cgcctcattc acccagccat cgaactgctg ctggtgggct
    24541 ttgatccagc catcaacgtg gccctgaata tcgccttctg aggctttgcc gtcatgcata
    24601 atggcgttct gggcgttaat atctgccact ggcaactgca taatggcaaa cagtttcgct
    24661 gctgccgggt ttttctcggc ccaggctttg ttggcaacga tatgcatggt gctgaccggg
    24721 aagccataat tcgcaccatt cggcagtttg gtatcggcgt ttttatcgcc cggcagtgcg
    24781 gagaacggca cctgcaacca gacgacatct ttgcccggct tcagttcgtt actcacccag
    24841 tacggcgtcc aggtgtagta aaacaccggt ttgccctctt tgtagcgact gatggtgtcg
    24901 gccatcatcg ccgcgtagtt cccctgatta tgcgtcacgg tatgggtcag ttcatacgcg
    24961 gcaagctggt ggttgatcgc accttcgcag ccccagccag ggttacaacc ggttaaatcc
    25021 gcttttccgt cgccgttggt atcgaacagt ttggcgatct tcggatcttt cagttgtgcg
    25081 atgttggtga ttttgtactg gtcggcggtt ttcttatcga tcaggtaacc ctgtgccgcg
    25141 ccgttaacaa ataccccttc acgataaaat ttcttatcgc caccggcagc ttcgtacatg
    25201 ttgtcatgca gtggcgtcca gttcacggcg gtgaaggttg catcgccgga agcaagcgag
    25261 gtgtagccaa cgttgtaatc cacttcgctg ggtttgttga cggtataacc taatttctcc
    25321 agcgcacgac tgaccagcag cgtctggaag gtttcttcgg tgatggtgct ttgaactgga
    25381 ttaacagtaa tacctttgcc cggcagatcg gcagcaaaag tttgtgtaga gataagcgtg
    25441 gcaaacgctg tcgcaaaaag tacgctatgt cgcattgtta ttcctttttt tggcagggtg
    25501 ataatgcccg gcgtcaccgg gcaagtgcag agttacttaa tgaatgggcg ggtcagcaga
    25561 ccaacagggc cagtggtgta ccagcgacgg ttgccgcgac tgcgtgagtc gcgcccaacg
    25621 gcctgcgtca ggcgatcgag gataatggcg aggatcacaa tcccgacgcc gccaacggtg
    25681 gcaagcccca tatccagacg accgataccg cgaagtacca tctgacccag cccgccgacg
    25741 gcaatcatcg aggcaatgac caccatagaa agggccagca tcagcgtctg gttaacgccc
    25801 gccataatgg tcggcatcgc cagcggtaac tgaactttga acagcatctg gcgcgggctg
    25861 gcaccgaatg agcgcgaggc ttcaatcaga tccgccggaa cctggttaat ccccagaatg
    25921 gtcagacgga taatcggcgg cagagcaaag atgatcgtca ccaccacgcc cggcacgtta
    25981 ccgataccaa atagcatgac gattggcacc agataaacaa acgctggcgt ggtctgcatg
    26041 gcatcaagca gtggacgaat aattttcgcc gctcgcggac ttctcgccag ccagatcccc
    26101 aacggcaaac cgatgacgat acagaacagc agggcggtta acaccagcgc cagagtaacc
    26161 attgcctgcg accaggcacc gattgcgccg atggcaatca gcgaaaccag cgtcgccacg
    26221 cccattccga ccccggaaat ctgccaggcg atgagagcga aaacgataat cgccaccggt
    26281 gcgggcatac ccagcagcaa ctgctggaaa ccgttgagga tataatcaac cggaacgcgc
    26341 acgccctgga agacgggacg gaaatgggta acaacccagt cgatcccttc agtgacccaa
    26401 ctgtcgagcg ggatcagcgt tttatggaac ggatcgagaa tattaaaatg ctcgacgttt
    26461 ggcgcaggcg tactggtcag ccagtcagca ccaccgccgt cagtcggtgc agtcgccggt
    26521 gtaccccagg cgtctgcgga ttgcgcggca ctgtccgccg ctggcgtggt atcccacgga
    26581 ttattttgat cagccattat ttaccccctc acgatctaaa gcgcgcagca gcattccttt
    26641 cgaaatgatg ccgacatact gttggtcctc gtcgaccacg ggcaccgcac agggtgcctg
    26701 tccgacatga gagagcaact cgctaagagg cgtttgtgca tcgactgcta acggcgcatc
    26761 aatcagcgcc gcatcaagac cttgctgctg cgttaacgcg gttttaagcg aatcgatgga
    26821 gactgcgccg acaaacttat taccgcgttc gataacgtag ccatattcgc gatcttcatc
    26881 ctgcaataat ttcagtgccg aacgtgggcc gaagccaggg gttttacgaa ttaagccatt
    26941 cggtgtccgg cgggcaatat ctttcgcact gaatacctga ctaatatcaa cgccacggaa
    27001 gaaggtacgg acataatcat tcgccggatt attgagaatt tcatccggtg tgccgacctg
    27061 taccacttca ccattttgca taatggcaat tcggtcgcca atacgcatag cttcatcgag
    27121 atcgtgggaa ataaagacaa tggtgcgctg atgtttcgcc tgtaatttta ccagctcatc
    27181 ctgcatctcg gtgcgaatta atggatcgag cgccgagaag gcttcgtcca ttaataatat
    27241 atccggatta atcgctaacg cgcgggctaa tcccacacgt tgacgcatcc cgccagagag
    27301 ttcatccggg tagctgtggg cataattttc cagcccgacc tgacgcagtg catcaagggc
    27361 tttttcccgg cgttcttcgg cattaattcc ggccaattcc ataccgaacg cagtattgtc
    27421 cagcacggtc atatgcggca ttaaggcaaa ggactggaag accatcgcaa tcttttttct
    27481 gcgcacctca cggagttcgg catcggatat tttggcaata tccacaccat caatcagcac
    27541 ttgcccgcgg gtgggttcaa tcaggcgatt gagaaggcgt accattgtgg atttacccga
    27601 gccggataat cccatgatga caaatatctc gccttcttca atggccagac tggcgtcttt
    27661 tacgccaagc gatagcccag ttttttccag aatttgttct ttcgaaagtc cttgttcgat
    27721 atatttgaac gctcgctgtg gatgctcacc aaatatttta taaagatttt taatttctaa
    27781 tttaattgcc atgcaataga aagattcctt tatttgttta tgtcgatata ttacccaatg
    27841 gaaatagtca cttttttcta ccctaacata ctgagaatct gaggcaaccc ctgagggcaa
    27901 atgtggcatg aatattgatg cgagtaaatc gccggaattt cccgtgatat aagggctgag
    27961 agcaaatcga gaaaagttga tattttttgt caaagatagc gaaaaaacgc ctgaatattg
    28021 tggtttcagg cgaataatgc aacagagtat cagtgtagat caccacaaaa tatttgcgtg
    28081 ataaaaagct atttggcggg aattatttcc ctgtcgcggg tagtgatatt tttgaaaata
    28141 acaccctcag aaattccagt cttcatcttc tgtttcaacc gctttcccca tcacataaga
    28201 ggagcctgaa ccggaaaaga aatcgtgatt ttcatcggca ttcggcgaaa gcgcggcgag
    28261 gattgccgga ttcacttccg ccatttctgc gggaaataaa ggttcgtagc ccagattcat
    28321 caaagcctta ttggcgttgt aacagagaaa cgctttcaca tcgtcagccc acggggtttc
    28381 ggcgtacagc tcatcggtgt attgcaactc gttgtcgtag agttccagca acaaatcgaa
    28441 ggcgaaactc ttcaactctt cacgttgtcc cagagatatc ttttccatgt ttttctgata
    28501 tttatagcct atgtagtaac cgtgtactgc ttcatcgcgg ataatcagac ggatcaggtc
    28561 tgcggtattg gtcagctttc cgcggctgga aaaatacatc ggcagccaga aaccggaata
    28621 gaacaaaaaa gattcaagaa acacactggc gattttcttt ttcagcggat catcaccgcg
    28681 ataatgttgc tgaataatct gagcttttcg ctgcaacggt gcgttttctt cactccaggc
    28741 gtaggcggca tcgacatctt tggtctggca cagcgtcgag aaaatcgaac tgtaagagcg
    28801 ggcatgaacc gcttccataa agctgatatt cgataatacc gcttcttcat gaggcgtgag
    28861 tgcatcgggc atcagcgaag gcgcgccgat aacattttgc agcgtgtcga gcagcgtcag
    28921 gccagtaaaa acgcgcatcg tcagttgttg ttctacgaca gttaatgtct gccaggcagg
    28981 aatatcgttc gacagcggca ccttttctgg tagccagaaa ttgctggtca ggcgattcca
    29041 cacctccaga tctttatcgt cagatatctt gttccagttg atggcgctga tacgtgagag
    29101 tttcatagat attccttaaa gtgcacagga gacgcagcct tcaatttcag tgccttccag
    29161 cgccatctga cgcaggcgga tgtaatagag cgttttgata cctttgcgcc aggcgtatat
    29221 ctgcgctttg ttgatatcgc gagtggtggc ggtatcgggg aaaaacagcg tcagcgacag
    29281 cccctgatcg acatggcgag tcgcttccgc gtaggtgtcg atgatctttt ctgcgccaat
    29341 tttgtaagcg tcctgataca gcgccagatt ttcgttagtc ataaacgggg cagggtagta
    29401 aacgcgtcct gttttgccct ctttgcgtat ctctactttc gccacaatcg gatgaatact
    29461 cgacgtagca tggttgatat aagagataga accggttggc ggcaccgcct ggagattctg
    29521 gttgtatatg ccgtagcgca tcacgtcgtc gcgcagttgc gcccacatct cacgggtagg
    29581 taacgtaata ccgctgcggg caaacagttc gccaactttc gccgttttcg gctgccagtt
    29641 accttgcaga tattggctga aatattcacc actggcatag cgtgactgtt tgaacccggc
    29701 gaaggtttca ccgcgttcgc gcgccagcaa catcgaggta cgcagtgcgt gccaggtgat
    29761 ggtatagaaa tagagattgg tgaaatccag ggcttccggc gaaccataag cgatgccttc
    29821 cctcgccaga taaccatgta aattcatctg ccccagtccg atggcgtgcg aggcggcatt
    29881 tccggcttcg atggacggca cgctgcggat atgactcata tctgacaccg ctgttaaacc
    29941 acgtacggca gtctctaccg tgcgggcaaa gtcgggggaa tccatggtgt gagcaatatt
    30001 caacgaacct aaattgcagg aaatatcatg gcctgtacgg gcatagtcga gattctcgtc
    30061 atactctgag gcgctgttaa cctgcaaaat ttctgagcag agattactca tatttatgcg
    30121 cccggcgata gggttagcac ggtttaccgt gtcttcatac atgatgtagg gatagccgga
    30181 ctcaaactgg atttctgcca gtcgctggaa gaaatcacgg gcgttgaggt attttttgcg
    30241 aatgcgttca tcggcaacca gttcgtcata gtgttggctg atggccacat cggcaaacgg
    30301 cttgccataa actagctcaa cgtcataggg cgaaaacagc gccatctgcg cattctcttt
    30361 tgccagatgg aaagtgatat ccgggatcac cacgcccagc gacagtgttt taatgcggat
    30421 tttctcgtcg gcattttccc gtttcgtatc gagaaaacgc agaatatcgg gatgatgggc
    30481 atgtaaatag actgcaccag ccccctgacg agcgccgagc tggttggcat aggaaaatgc
    30541 gtcttccagc attttcatca ccggaatcac gccagaagat tgattttcaa tacgtttaat
    30601 tggcgcgccc gcttcccgca gattcgacag caaaaatgcc acgccgccgc cgcgtttcga
    30661 cagttgcagt gcggaattta ccgcccggcc aatcgactcc atattgtctt caatacgcag
    30721 caaaaaacag gaaaccagtt cgccgcgctg ctgcttacca cagttgagga atgtcggtgt
    30781 tgcaggctga aagcgtcctg acagcatctc atcggtcagt tgcaacgcca gtgtttcatc
    30841 gccttgtgcc agcgttagcg ccaccatcgt gactcgatcg gcaaaatctt ccagataacg
    30901 cttaccgtcg aatgtcttca acgtatagct ggtgtaaaac ttccatgccc cgaggaatgt
    30961 atggaaacga aaaccgctgg tgtgtgcgtg ggcaaacagc gtaatgacaa agtcgcgaga
    31021 gtagcgatta agaacgcttt catcatagta accttcgttg accagccagt tcaggcgctg
    31081 ctgctggcta ctgaaggtca cactgttcgg acgcacatgc gtcgccataa aggcgtcaac
    31141 ggcctggcgg tctttatcga actgaatgcg acctgcgcta tcgtagaggt taagcatcgc
    31201 attcagcgcg tggtaatcca tcgtttcctg cgtcaggcat tctgcggttg tcgttgccaa
    31261 aattcggtta ctcctttacg aacgttttcg atatcgcttt gcgtacccat gagttcaaaa
    31321 cggtacagcc acggcacgcc gcatttctga gcaatcacat ctccggcacg gccatacgcc
    31381 tcaccaaagt tgcgattacc cgaagcaata acgccgcgaa gcaacgcccg gttgtgctcg
    31441 tcgtttaaaa agcgaattac ctgtcgtggc accgcgccag ccgtaccgcc gccgccgtaa
    31501 gagggcacga tcaggatgta aggctcgtct acctgaatcc gttcccgctc attgagcggg
    31561 atgcgcaccg cgggcagacc taaacgttcg ataaaacgct gcgtattttc ggagctgctg
    31621 gagaagtaga cgagctggct catgcactgg ccgcgtgtgg cgctggatgc agacggttaa
    31681 tcatgtccgg acggaaacca gaccagctaa gatcgccagc aatcactacc ggcaactgac
    31741 gaaagccctg agcacgcaac gcttccgctg cttcaggaac gcgatcgaca ttaatcattt
    31801 caaaatcaaa gccccggttt tccatcgccc gtttggtggc gtggcactga acgcaatcgt
    31861 tacgagtgta aatagtaatg cgcatgattc gtatttccgt ttaaaatgaa gatacggcgc
    31921 gatgatacgc gtcgggttgt ctctctgttg atacagagat actagatgta gttgaaaaaa
    31981 gattcaacca cacaatatat agcaaatagt ggtcgaaatt accctggata tgagcgtgac
    32041 ggggtagggg gatttttgtg attcaccagg caaaaagaaa ccccgaagac aggcttcggg
    32101 gtcaaagacg cgtatttatt atcatttttg cactacgatt tgcgcatgct taacagtgcg
    32161 ccgataaaaa tacctaccgc agctgctgta cccacgctac accagggtct ttcacgaaca
    32221 aaagaatccg cgcagccaac ggcatcgcgc gcggcttgct ggacgcgagt acgaccatgc
    32281 attcgcgccc tggtttcttt cagcaatgcc tgagctttac tgcgtgcggc ttcagcttcc
    32341 cctttggcgt cgctgcccca ggactttaat acagattcga ggctgtcagc taattgattg
    32401 acatcattct ggatatcctg cacgccatca tcaacgtcgt tgcggttcgg tcggttaaac
    32461 atatgatccc ccattagaat tcgatgtaat tttcagtgta gaccataatt ttcttaactg
    32521 aggttcatcg cggctttatg gacttttctg aaagcgttgt gaatgcgcaa tcaggtaagg
    32581 taggcccgcg gttgataata acgaggatca aatatgtatt tacgaccaga cgaggtggcg
    32641 cgcgtacttg aaaaagtcgg ttttactgtc gatgtggtaa cccagaaagc gtatggttac
    32701 cgccgtgggg aaaattatgt ctatgttaat cgcgaagcgc gaatgggacg taccgctctg
    32761 gtaattcatc caacattaaa agaacgcagt tcaacactgg ctgaacccgc ttccgatatt
    32821 aaaacttgcg atcattacca gcaatttccg ctctatttag caggcgagcg acacgagcat
    32881 tacggtatcc cgcatggctt cagttcgcgt gttgcgcttg aacgttattt gaatggttta
    32941 tttggcgaag ccagttaaag acgcgactgg cgatgccagt cgcgaaaaga agagtcaggc
    33001 ttttacctgc tggtaacggc tgactttaaa cagtcggcgg caataatcga ggaagtagcc
    33061 ataaaccgcc cccatcaaca tcgaaacaac gatgtttgaa ctgaccgccg ccataatctg
    33121 atgccagtct gcgcccacca ctaacaagat cgccacatac accggtgact ggaacgtcac
    33181 ataagccagg atatcagcca gattttttat ccagcccgac gggctaactt tgcgtgccgc
    33241 gcgcataaac agatcgcggt acataccgta tggccatgca attaagatgt tcaccggaat
    33301 cgctaccaat ctggaataaa aagactgttc gaagctcatt ccggagagga aaacttcaat
    33361 acacatgttc acgacagaac agtaaacaac catcgcgaac gtatctgcaa ctgcatgacg
    33421 caagcgtgac tgcggtgaga acatgctaat gctccttaat aaaagcgtaa tgggatgtta
    33481 atggagatga aagcagtgga tctcattgcc atctgcattt tcgcatagcg tatatcgctg
    33541 actatgacta atcaattagt gaaaaacttt tatttagtca gtttt
//
LOCUS       DAJOWB010000041        33001 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJOWB010000041 DAJOWB010000000
VERSION     DAJOWB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.041"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(236..1828)
                     /gene="lsrK"
                     /locus_tag="OHO85_002144"
     CDS             complement(236..1828)
                     /gene="lsrK"
                     /locus_tag="OHO85_002144"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HCQ2671514.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     CLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1907..2860)
                     /gene="lsrR"
                     /locus_tag="OHO85_002145"
     CDS             complement(1907..2860)
                     /gene="lsrR"
                     /locus_tag="OHO85_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCQ2671515.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3109..4644
                     /gene="lsrA"
                     /locus_tag="OHO85_002146"
     CDS             3109..4644
                     /gene="lsrA"
                     /locus_tag="OHO85_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCQ2671516.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMTDRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4638..5666
                     /gene="lsrC"
                     /locus_tag="OHO85_002147"
     CDS             4638..5666
                     /gene="lsrC"
                     /locus_tag="OHO85_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCQ2671517.1"
                     /translation="MLKFIQNNREITALLAVVLLFALPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFLNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5666..6658
                     /gene="lsrD"
                     /locus_tag="OHO85_002148"
     CDS             5666..6658
                     /gene="lsrD"
                     /locus_tag="OHO85_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCQ2671518.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6670..7692
                     /gene="lsrB"
                     /locus_tag="OHO85_002149"
     CDS             6670..7692
                     /gene="lsrB"
                     /locus_tag="OHO85_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HCQ2671519.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7719..8594
                     /gene="lsrF"
                     /locus_tag="OHO85_002150"
     CDS             7719..8594
                     /gene="lsrF"
                     /locus_tag="OHO85_002150"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCQ2671520.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8618..8908
                     /gene="lsrG"
                     /locus_tag="OHO85_002151"
     CDS             8618..8908
                     /gene="lsrG"
                     /locus_tag="OHO85_002151"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCQ2671521.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8965..9723
                     /gene="tam"
                     /locus_tag="OHO85_002152"
     CDS             8965..9723
                     /gene="tam"
                     /locus_tag="OHO85_002152"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCQ2671522.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLQQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPVQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9727..10641)
                     /locus_tag="OHO85_002153"
     CDS             complement(9727..10641)
                     /locus_tag="OHO85_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family inner membrane protein"
                     /protein_id="HCQ2671523.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10848..12299)
                     /gene="uxaB"
                     /locus_tag="OHO85_002154"
     CDS             complement(10848..12299)
                     /gene="uxaB"
                     /locus_tag="OHO85_002154"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCQ2671524.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12526..13944)
                     /locus_tag="OHO85_002155"
     CDS             complement(12526..13944)
                     /locus_tag="OHO85_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCQ2671525.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRMLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14083..14442)
                     /locus_tag="OHO85_002156"
     CDS             complement(14083..14442)
                     /locus_tag="OHO85_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCQ2671526.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14442..15368)
                     /gene="glsB"
                     /locus_tag="OHO85_002157"
     CDS             complement(14442..15368)
                     /gene="glsB"
                     /locus_tag="OHO85_002157"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCQ2671527.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15432..16820)
                     /gene="sad"
                     /locus_tag="OHO85_002158"
     CDS             complement(15432..16820)
                     /gene="sad"
                     /locus_tag="OHO85_002158"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HCQ2671528.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16921..17802
                     /locus_tag="OHO85_002159"
     CDS             16921..17802
                     /locus_tag="OHO85_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2671529.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17880..18994
                     /gene="yneK"
                     /locus_tag="OHO85_002160"
                     /pseudo
     CDS             17880..18994
                     /gene="yneK"
                     /locus_tag="OHO85_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            19144..20334
                     /gene="ydeA"
                     /locus_tag="OHO85_002161"
     CDS             19144..20334
                     /gene="ydeA"
                     /locus_tag="OHO85_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCQ2671530.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGSGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(20359..21024)
                     /gene="marC"
                     /locus_tag="OHO85_002162"
     CDS             complement(20359..21024)
                     /gene="marC"
                     /locus_tag="OHO85_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCQ2671531.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            21236..21670
                     /gene="marR"
                     /locus_tag="OHO85_002163"
     CDS             21236..21670
                     /gene="marR"
                     /locus_tag="OHO85_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCQ2671532.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21690..22073
                     /gene="marA"
                     /locus_tag="OHO85_002164"
     CDS             21690..22073
                     /gene="marA"
                     /locus_tag="OHO85_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCQ2671533.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            22105..22323
                     /gene="marB"
                     /locus_tag="OHO85_002165"
     CDS             22105..22323
                     /gene="marB"
                     /locus_tag="OHO85_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCQ2671534.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(22380..23819)
                     /locus_tag="OHO85_002166"
     CDS             complement(22380..23819)
                     /locus_tag="OHO85_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCQ2671535.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(23844..25517)
                     /locus_tag="OHO85_002167"
     CDS             complement(23844..25517)
                     /locus_tag="OHO85_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HCQ2671536.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(25573..25884)
                     /locus_tag="OHO85_002168"
     CDS             complement(25573..25884)
                     /locus_tag="OHO85_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCQ2671537.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(25912..27234)
                     /locus_tag="OHO85_002169"
     CDS             complement(25912..27234)
                     /locus_tag="OHO85_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCQ2671538.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            complement(27349..27660)
                     /locus_tag="OHO85_002170"
     CDS             complement(27349..27660)
                     /locus_tag="OHO85_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCQ2671539.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            27859..28557
                     /locus_tag="OHO85_002171"
     CDS             27859..28557
                     /locus_tag="OHO85_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCQ2671540.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(28602..29501)
                     /gene="eamA"
                     /locus_tag="OHO85_002172"
     CDS             complement(28602..29501)
                     /gene="eamA"
                     /locus_tag="OHO85_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCQ2671541.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            29696..30883
                     /gene="ydeE"
                     /locus_tag="OHO85_002173"
     CDS             29696..30883
                     /gene="ydeE"
                     /locus_tag="OHO85_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCQ2671542.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVKLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            31010..31105
                     /gene="mgtS"
                     /locus_tag="OHO85_002174"
     CDS             31010..31105
                     /gene="mgtS"
                     /locus_tag="OHO85_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCQ2671543.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(31324..32214)
                     /gene="dgcZ"
                     /locus_tag="OHO85_002175"
     CDS             complement(31324..32214)
                     /gene="dgcZ"
                     /locus_tag="OHO85_002175"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HCQ2671544.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(32469..32861)
                     /gene="ydeI"
                     /locus_tag="OHO85_002176"
     CDS             complement(32469..32861)
                     /gene="ydeI"
                     /locus_tag="OHO85_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCQ2671545.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         8660 a         8166 c         7636 g         8539 t
ORIGIN      
        1 agtttattaa accccttttg catccattag tgtctttagg aatattcgct ataaaataag
       61 ggttgtgctt aatgctttaa gaaaaatagc aattttccct tgaatatagt actggtgatg
      121 gaacgatgaa tctgcaagct ggcttttaac aagccagctc taaaagaagg gaaataagaa
      181 taactatact caaaaactaa cagccacggt catcatgatg tggctgtcaa tgaaactata
      241 acccaggcgc tttccataac gacgtcgtca gtccatgatc aaccagcccc agctgatcct
      301 gataaactgc ctgccactta tcgcgtgaat cctgataaag ttcatgcttt tccgggtctg
      361 gtgtgtgcgt ccgttcccag cgaaccaggc gttctccggt ttctgccatt gatgaaaaaa
      421 ttccggcacc gacgccagct gcaatggcac atcctaatgc agtggcttct ttgaccaccg
      481 gaatattgac gggtaatccc gagacatcag cgagaatttg actccataat ttcccttttg
      541 aacctccgcc tgcaaagact aacgatgaag gatgaatatt cgagaaatca gcaatttgct
      601 gcaagttaca cgctgataca atcgccgcat tttcttccag cgcacggaac aatgtcgctt
      661 tgttacattt atccgggtca atggacaagt taataaagga aggcgcagcg tgataccagg
      721 ttttaaagcg cattctgtcg gagaagatcg gcattacgcc ccacgaccca ggcggcaccc
      781 gactggccat ctcttccagc agcgtatagg tgtcgatgcc taaacattcg gcaatcagtt
      841 tttcttcggc acagaaagca tcgcggaacc agcgcatggt gagtccggta aaaaagctta
      901 tagattcagc ttgtaccatg ccaggaataa catgaggatt aacgcgcacg ttcatttctg
      961 ggtctgtcac cggcgcggct aaatttacaa cttgctgcca gaatgtgccg ccaagaaccg
     1021 cggtttgtgc cggacgcaca acgcctaacc caaggcaacc aagctgcacg tcgcctcctc
     1081 caacgaccac cggagtgccc gccttcagac cgcagagttc cgccgcttgt gaacttacca
     1141 cgcccagcaa tgtgccggtt tctttgacag gagaaagaat atcggcacgt aggccagcca
     1201 tatccagcaa tgcaggtttc cagtcacggg tggttagatc aagaagtccc gtggtgccag
     1261 cgttagaggg atccaccgcc agttcgccgc tgagcatata ggccagccag tcgctgatca
     1321 tggtgatggt tgatgcctga cggtaaatat cggaacgatg gtgcgccagc caaagtaatc
     1381 tggggatggc acttaaagcc agtgtttgtc cggtcgcgcg ataaacttcg ttttcaaagg
     1441 tattgttgtg cagttcttta agttcgctaa cttcgcgtgc cgctctggca tccacattgg
     1501 cgcaggccca gatcggggct ccttcattat tatataaaac aatgccttca cgcatcgaac
     1561 atgccgaaac ggcagcgata tactccgggg ctatgccggc gttgtgcagc gcctggcgca
     1621 tacactcaca cgccagttgc cagtttttgt tgagatcaaa ttccatagaa ccaggaacgt
     1681 ccggtactgc caggtgccgc cactccgcct gtcccactgc tatttgattg ccttccaggt
     1741 cgaatatcac agcccgaata cttccggtac ctgcatccag cgccatcagg taatactttg
     1801 attctgaagg ggtaaagagt cgagccatta gattatcctc ggcttatgcg ttctgtctgt
     1861 tcctctatac gttctccatc attcccggta ataaggtcat gcaaatttaa ctacgtaaaa
     1921 tcgccgctgc tgtgtcctga tcggtaacca gtgcgttgat ataaccgcct ttcattgcag
     1981 cggcaattgc ttcggcttta ttttctcccc ctgccacgcc aacccggacg ggtatggtct
     2041 tcagcgagct taaaggtaag ccaatcagtt cgttatgtat tttaatatcc gtgacaacgt
     2101 caccttttgc atcaaaaaag tagcctaaaa tgtcgccaac cgcccctttt cgaccaatca
     2161 ttaactgttc gccctggctg atataaccgg agcgaatgat cgtcgcgtcg tcctgttgac
     2221 tgacggcccc aatgccgaca atcgccacat cggctgcttg cgcggctaac agaacatctt
     2281 tgacgcaatt ttcatttttt agcgtacggg caatgtcagc ggaggatgcc cgcaacggag
     2341 ccggaataat attcacactg cacgccgcgt taagctgccc gatacccgtc atataagaac
     2401 cgacgccacc ggagagcgtg accaggcgaa tttgctgtga cgaaataaaa ccacttaagc
     2461 gttgcagcgt attcatggtt gcctcgccaa aaccaatcgc cagcatctgt tgtggttgaa
     2521 gtaaactcat caacatatgc gccgcgccta tccccagtcg cccgccgaca tcagcatccg
     2581 caagcccagg gatcacccgg acatgttgca gcgaaaactg acgacgtaat tgagtttcat
     2641 attccagaca gccttcaaag cgagaattaa tctgtacgcg aataatgccg gactgatgcc
     2701 ctttctccag caagcgcgac actttcaaac gggtcaggcc gagacgatcg ctgatctcgc
     2761 tctgggtcag cccgtcgtga tagtaaaacc acgcgatccg cgcgacctgt tcttcttcac
     2821 acattccctg ttctgaaatt gccgaatcgt tgattgtcat aattcattct tcactttgaa
     2881 catatttaaa tctttaatgc aattgttcag ttcttgcgca tttatatctg tgatggcagc
     2941 cacagtttga ctctacgagc atgaacaaac gcaaccgtga aaatcaaaat agcataaatt
     3001 gtgatctatt cgtcggaaat atgtgcaatg tccacctaag gttatgaaca aattaaaagc
     3061 agaaatacat ttgttcaaaa ctcacctgca aaactgaacg ggggaaatat gcaaacgagt
     3121 gatacccgcg cgttaccgct actttgcgcc cgctcggttt ataaacagta ttcaggggtc
     3181 aatgtcctga aaggcatcga ttttacgttg catcaggggg aggtccacgc cctgctcggc
     3241 ggcaatggtg ccggtaaatc gacgttaatg aagattattg ccggtattac ccctgctgat
     3301 agcggtacgc tggagattgg gggcaacaac tacgccagat taacgccagt tcatgctcat
     3361 cagctgggca tttatctcgt tccccaggaa ccgctgcttt tcccaagcct gtcgataaaa
     3421 gaaaacatcc tgtttgggat ggcaaaaaaa cagctctcca tgcaaaaaat gaagaacttg
     3481 ctggcggcgc tgggctgcca gtttgatctg catagtctgg caggatcgct ggatgtcgcc
     3541 gatcgccaaa tggtggaaat cctccgcggg ctgatgcgcg actcgcggat tctgatcctc
     3601 gatgaaccta ccgcctcgct tacccctgcg gaaaccgaac gcttgtttag tcgcttgcaa
     3661 gagctgcttg ctactggcgt gggtattgtt tttatctcgc ataagctgcc ggaaattcgc
     3721 cagattgccg atcgaattag cgtgatgcgc gacggaacca tcgccttaag cggcaaaacc
     3781 agcgaactgt ctaccgacga cattattcag gccatcaccc cagcggtacg ggaaaaatcg
     3841 ctctctgcca gccaaaaatt atggctggag ttacctggta accgcccaca acatgctgcc
     3901 ggaacgccgg tgctgacact ggaaaatctg accggcgaag gtttcaggaa tgtcagcctg
     3961 acgctcaatg ccggagaaat tctgggcctg gctgggctgg tgggagccgg acgcacagaa
     4021 ctggccgaga cgctctatgg tctgcgtact ttgcgtggcg gacgcattat gctgaatggt
     4081 aaagagatca ataaattatc cactggagaa cgtttactgc gcggtctggt ttatctgccg
     4141 gaagatcgcc agtcatccgg actgaatctc gatgcttcgc tggcctggaa cgtctgcgcc
     4201 cttactcata accttcgtgg attctgggcg aaaaccgcga aagataatgc caccctggaa
     4261 cgttatcgtc gggcgctgaa tattaaattt aaccaaccgg aacaagctgc acggacatta
     4321 tccggtggca accagcaaaa aatcctcatt gccaaatgcc tggaagcttc gccgcaagta
     4381 ttgattgtcg atgagccgac gcgcggcgtg gatgtctcgg cacgtaatga tatctaccag
     4441 ctgttgcgca gcatcgccgc acagaatgtg gctgtgctgc ttatctcctc tgatctggaa
     4501 gagatcgaac tgatgacaga tcgtgtgtat gtgatgcatc agggcgaaat tacccactct
     4561 gcactgaccg ggcgcgatat taatgtcgag actattatgc gcgttgcctt cggcgatagt
     4621 cagcgtcagg aggcatcatg ctgaagttta ttcagaacaa ccgtgaaatc acggcactgc
     4681 tggcggtggt gctgctgttt gcattacccg gttttctcga ccgccagtat ttaagtgtgc
     4741 aaacgctgac catggtttat agcagcgcgc aaatcctgat cctgctggca atgggcgcga
     4801 cgctggtaat gcttacgcgc aatattgatg tttcagtggg ttcgattacc ggaatgtgcg
     4861 cagtgctgtt ggggatgtta ctgaacgcgg ggtattcact acctgttgct tgtgtcgcga
     4921 ctttactgct tggtttgctc gcgggatttt tgaacggtgt cctggtcgcg tggctaaaaa
     4981 tccctgccat tgttgccacc cttggcacgt tagggttgta cagaggcatc atgttgctgt
     5041 ggactggcgg taaatggatt gaagggttac ccgccgaact gaaacagctc tccgcaccgc
     5101 tgctgtttgg cgtttccgcg attggttggt tgacgataat tctggtggca tttatggcct
     5161 ggctgctggc aaagacggcg tttggacgca gtttttatgc cacgggcgat aatttacagg
     5221 gcgctcgtca actgggtgtt cgtacggaag ccattcgcat tgtggcattt tcgttgaacg
     5281 gctgcatggc ggcactggcg ggaattgtgt ttgcttcgca gattggtttt atccccaacc
     5341 agaccggtac cgggctggag atgaaagcaa ttgcagcctg cgtgctgggc ggcattagtt
     5401 tgctcggcgg ttccggtgcg atcatcggtg cggtactcgg cgcatggttc ctgacgcaga
     5461 tcgatagcgt actggtgctg ttgcgcattc cggcatggtg gaacgatttt atcgcgggcc
     5521 tcgtgctgct ggcggtgctg gtgtttgatg gacgcctgcg ttgtgcgctg gaacgtaatc
     5581 tacggcggca aaaatatgcc cgctttatga cgccaccgcc atccgttaaa cccgcttcgt
     5641 caggtaaaaa acgggaggcc gcataatgcg tattcgctac ggttgggaac tggctcttgc
     5701 cgcactgctc gttattgaga ttgtcgcatt tggtgcaatt aacccgcgaa tgttagatct
     5761 caatatgttg ctgttcagca ccagtgactt tatctgcatt ggcattgtcg ccctaccgct
     5821 gacgatggtg attgtcagtg gcgggatcga tatttcgttt ggttcgacca tcggcctctg
     5881 cgccattgca ttgggcgtac tgtttcaaag tggtgtgccg atgccgctgg cgattctcct
     5941 gaccttactg ctcggcgcat tgtgcgggct gatcaacgcc ggattaatta tctataccaa
     6001 agttaacccg ctggtgatta cgcttggcac gctgtatctg tttgccggaa gcgccctact
     6061 gctttccggg atggccggag cgacggggta cgaaggtatt ggtggattcc cgatggcgtt
     6121 tacagatttc gctaacctgg atgtgctggg actccccgtt ccgctgatta tcttcctgat
     6181 atgtctcctc gttttctggc tctggctgca taaaacccat gccggacgta atgtgttttt
     6241 gattgggcaa agcccgcgcg tggcgcttta tagcgcgatt ccagttaacc gcaccttatg
     6301 tgcgctctat gccatgacgg ggctggcgtc tgcggtcgcc gctgtgctgc tggtgtcgta
     6361 ttttggttca gcacgttccg atctcggtgc gtcgtttctg atgcccgcca tcaccgccgt
     6421 ggtgcttggc ggtgccaata tttatggtgg ttccggttcc attatcggca ccgccattgc
     6481 ggttttatta gtgggatatt tgcaacaagg tttgcaaatg gcaggagtgc caaatcaggt
     6541 gtccagcgcc ctttccggtg cgctacttat cgtcgttgtc gtaggtcgtt ccgttagcct
     6601 gcatcgccag caaattaaag agtggctggc gcgtcgggcc aataacccat tgccataaag
     6661 gatatcttca tgacacttca tcgctttaag aaaatcgcct tacttagcgc tcttggcatt
     6721 gccgcaatct ctatgaatgt gcaggccgca gagcgtattg catttattcc caaactggtt
     6781 ggcgtgggat tttttaccag cggtggcaac ggcgcacaac aagcgggtaa agagctgggc
     6841 gttgatgtga cctacgacgg gccgacagaa cccagtgttt ctggtcaggt acagttgatt
     6901 aataacttcg tcaatcaagg ttataacgcc attatcgttt ctgcggtttc gcctgatggc
     6961 ttgtgtccgg cactgaaacg cgccatgcaa cgtggtgtga gagtgctgac ctgggactct
     7021 gatactaaac cggagtgccg ctcttactac attaatcagg gaacgcccgc ccagttggga
     7081 ggtatgttgg tggatatggc ggcgcgtcag gtgaataaag acaaagccaa agtcgcgttt
     7141 ttctactcaa gccccaccgt tacggaccaa aaccagtggg tgaaagaagc gaaagcgaaa
     7201 atcgccaaag agcatccagg ctgggaaatt gtcactacgc agtttggcta taacgatgcc
     7261 actaaatcgt tacaaacagc agaaggaata ttaaaagcgt atagcgatct cgacgccatt
     7321 atcgcccccg atgccaacgc cctgcccgct gccgcacaag ccgcagaaaa cctgaaaaat
     7381 gacaaagtag cgattgtcgg attcagtacg ccaaatgtga tgcgcccgta tgtagagcgc
     7441 ggcacggtga aagaatttgg cctgtgggat gtggttcagc aaggcaaaat ttcagtgtat
     7501 gtcgcggatg cattattgaa aaaaggatca atgaaaacgg gcgacaagct ggatatccag
     7561 ggagtaggtc aggtggaagt ctcgccaaac agcgttcagg gctatgacta cgaagcggat
     7621 ggtaatggca tcgtactgtt accggagcgc gtgatattca acaaagagaa tatcggcaaa
     7681 tacgatttct gatgtgcatt acttaaccgg agtaagttat ggcagattta gacgatatta
     7741 aagatggtaa agattttcgt accgatcaac cgcaacaaaa tatccctttt accctgaaag
     7801 gttgcggtgc gctggattgg ggaatgcagt cacgcttatc gcggatattt aatccgaaaa
     7861 cgggtaaaac cgtgatgctg gcttttgacc atggttattt tcagggaccg actaccggac
     7921 ttgaacgcat tgatataaat atcgccccgc tgtttgaaca tgccgatgta ttaatgtgta
     7981 cgcgcggcat tttgcgcagc gtagttcccc cggcgaccaa taagccggtg gtactgcggg
     8041 cgtcaggtgc gaactctatt ctggcggaat taagtaatga agccgtggcg ttatcgatgg
     8101 atgatgccgt gcgcctgaac agttgcgcgg tggcggcgca ggtttatatc ggcagcgaat
     8161 atgaacatca gtcgatcaaa aatattattc agctggtcga tgccggaatg aaagtgggaa
     8221 tgccgaccat ggccgtgact ggcgtgggca aagatatggt gcgcgatcag cgttatttct
     8281 cgctcgcgac tcgaatcgcc gctgaaatgg gggcgcaaat tatcaaaacc tattatgtcg
     8341 aaaaaggttt tgaacggatt gttgccggat gtccggtacc cattgttatt gctggcggta
     8401 aaaaattacc ggagcgcgag gcgctggaaa tgtgctggca ggctatcgat cagggcgctt
     8461 ctggtgtgga tatggggcgt aatattttcc agtctgacca tccggtggcg atgatgaaag
     8521 ccgtacaggc ggtggttcac cataacgaaa cggctgatcg ggcatatgaa ctctatctga
     8581 gtgaaaaaca gtaactgcgg atttaaggag aagaattatg cacgtcacac tggttgaaat
     8641 taacgttcat gaagacaagg ttgacgagtt tatcgaagtt tttcgccaga accacctggg
     8701 ctcggtacag gaagaaggca atttgcgctt cgatgtctta caggacccgg aagtgaattc
     8761 gcgcttttat atctacgaag cctataaaga tgaagacgca gtggcattcc ataaaaccac
     8821 gccccactac aaaacctgtg tcgcgaaact ggaatcttta atgaccgggc cgcgtaaaaa
     8881 acgtctgttc aatgggttga tgccgtgagg cgaatttatc aattttatct acaattgggg
     8941 taacgcgctg acgggagtaa aaaaatgtct gactggaacc cctctttata tctgcacttt
     9001 gccgctgaac gatcgcgtcc ggcggtggag ctgcttgcca gagtgccgct ggagaatgtc
     9061 gaatatgttg ccgatcttgg ctgtggccca ggtaacagca ccgcccttct acaacaacgt
     9121 tggcctgctg ccaggataac gggcatcgac tcgtctccgg cgatgattgc tgaagcgcgc
     9181 agtgctttgc cagactgcca gttcgtggaa gcagacatcc gcaactggca accggtacag
     9241 gcactcgatc tgatttttgc taatgcctca ctgcaatggc tgcccgacca ctacgaattg
     9301 tttcctcatc tggtttcttt acttaatccg cagggcgtgc tggcagtaca gatgccagat
     9361 aactggctgg agccgaccca tgtgctcatg cgcgaagttg cctgggaaca aaactacccg
     9421 gatcgtgggc gtgagccgtt ggctggcgtt catgcttact acgatatttt gagcgaagcc
     9481 ggatgtgagg tcgatatctg gcgaaccacc tactatcacc agatgccgtc acaccaggcg
     9541 attatcgatt gggtgaccgc cactggatta cgtccgtggt tacaggatct gaccgagagc
     9601 gaacagcagc tttttcttaa gcgctaccat cagatgctgg aagagcagta tccactgcaa
     9661 gagaacggac agatactgct ggcatttccg cgtcttttta ttgttgcccg ccgtacggag
     9721 taaatatcac gtcagctggt aatgacgatc gggaagaatt tttgctggaa tttcggcttc
     9781 atcgttcatc tgtaacaggt caatttcaat agcgttgcag atggcatcca gcggtaaatc
     9841 attgttttca gtaccgaacg gatcttccag ttcttccgcc agacaatcca gcgaaataaa
     9901 agtgtaggaa atcagcacag agataaaagg cgtcatgtaa tgcaggtcca cgaccagcgc
     9961 gaacggcagc atgatacaaa acagataaac ggtacgatgc aaaatcaggg tgtaggcaaa
    10021 gggaattggc gtataggcaa tgcgctcaca gcctgccaga accgctgaaa tatcattaag
    10081 ccgatcgttg aggctaataa acaggatatc tgaaagctgt ccattgcggc gctgaaccgc
    10141 caaccattct cccattatta acaagatacg gttagctgga gagttcgaag ccagtacacg
    10201 ctgaagatct tcagtcttga gataatgagc cagcacttcc gcctgtggct gtttgcgtaa
    10261 tgtcatgcgt aaacagtggg cgaaggcgat ttgcagccgg gcaaactccc ttacacttgc
    10321 cgaatccggc aatgtcgttt ttacctcgcg cagtaacgac cgtgaggcaa tcatcaactg
    10381 cccccaaagt tttcgcgctt caacgtaacg ggcgtacccg gcattattac gaaaaccaag
    10441 aaaaatggcg atggcgacac cgagaatgct gaacggtgcg agggtgaatt tgatgcccag
    10501 atgcgtgtac cagggcagca tgaatataac agcgatagaa aagagaaaat tgagtagtaa
    10561 gcgcgaggat atcttggata atactgagcc gtgccagaca aaaatacggc gcagccagtg
    10621 ttgttgtgga cgaacaatca tggttatctt caggcgtgga aaagtcgccc tattaaacgt
    10681 gattacgatc acattctcaa gacgctactt acaaattacc tacaagctta caacattaac
    10741 aggcgctgca tgtggcacct gttaatgata aaggcacata ggatgttgta actaatatag
    10801 tgaaataaga aacccggtcg aaaccgggtt cagaagtaac ggtgttatta gcacaacgga
    10861 cgtacagctt cgcgcatccc tttttcaaga atcgcatcca ggtcattagc aacctgctcg
    10921 accagaccag gcacttgcgt caggtcctgc tcccagtggt ctttttctgc caaaacaatc
    10981 gctaccagtt cctgggtgcc gataacacgg tcacgatgct ggctccagag ttgctgataa
    11041 cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt ctccgttacg ctcaccgcga
    11101 tagaaggcaa tcaatgccgc taatgcgaaa gtaaggcgcg ccggaagtgt gccttttgcc
    11161 ttctgccctg ccagcagctg cggcaggatg cgggtgcgga acttggtcat accgttgagc
    11221 gcgatagaca gcagctgatg cttaatgtac gggttacgga aacgcccggt gactgcactg
    11281 gcgaaagatt ccaattcatc acgaggcaaa tccagtaccg gaataatttc ttcgtagata
    11341 gctttttcaa cgaatgcgca aatttcagca tcgttcatcg cttcacctac ggtatccagc
    11401 cctgcctgga acgccaccgg caccagcgcg gtgtgagcac cgttgaggat tgccactttg
    11461 cgctctttat acggtttaat atcgtcaaca atcagcacgt tgagtggata tttgtccagg
    11521 cgcagctcag tcgctaagga tttcggcccc tgaatcacaa acaggtaaaa gtgttcagcg
    11581 gtgtcgagga aaccatcgtg ataacccagc tcttcttcca gtttagccac ttcatcgcgc
    11641 ggataaccgg taacgatacg gtccaccagc gtagaacaga agctgttagc ctgatccagc
    11701 cattgaataa atgcttctgg cagtgcccac tcttgcgcat agcgcagcac cagttcacgc
    11761 aacgcgtcgc cattgtagtc gatcaactca cacgggatga tgatccaacc tttatccagc
    11821 gcaccgttga aatggctaaa acgttcgaac agcagacggg tcagttttgc cggataactt
    11881 accgctggcg cgtcatcgaa tttatcgccc gcatggtagc tgatacccgc ttcggtggta
    11941 ttagagaaaa caaagcgcat ttccgggttg tgagccagtt tcaggaattc atcgtattca
    12001 ctgtagacgc tgatttcacg attaaccgag cgaatcagac gcgcgtcgct gaccgcttcc
    12061 cccttctcat tcaggccacg gataatggtg gtatacagac catcctgcgt gctcagtgac
    12121 ggtgggaatg aagtttcaat cggacgaaca acgaccacac cagaattcag atcggtgtgc
    12181 tcattcagga gatcgatttg ccagtcaaca aaggcgcgca ggaagttacc ttcaccaaac
    12241 tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc gacgatttag tgttttcaca
    12301 atgggttccc ttctgattag tcatacaacc tgtttgaatt ggtacgacag gttagcaaac
    12361 tttaatacgc cgaacccctg ttttgatcaa ctcctgatga ttaatgagca gttttatgag
    12421 aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg ccttttcctg gatcataaag
    12481 tggtagaaca cattgcattc aaatcgcgcg gaatgaataa agatgtcaga caacttcctc
    12541 accgtaacgc atagtgctgg tacggttgcg cccatctttc ttcgagcgat acagacacgt
    12601 atcggcttcg accatcaatt tattaaaatc atcggtcagc attcggtacg atgcgcgacc
    12661 actaccgacg ccaatactta ccgtgagata aagcgttttt tgttgccagg tgaatggttg
    12721 cagttcaacg cctttacgga ttttttccgc cattagcaga ccatcgacag gattcaccga
    12781 cggcactgca acagcaaatt cttcgccgcc catgcgcgcc accagcccct tatcaccgac
    12841 aatcttctga atatgccggg caaacacgct taacacttta tcgccacatt catgtccata
    12901 gttatcgtta atgcttttga aatagtcgat atcaagcagc atgaccgtca gatgttgtgt
    12961 ctgtttcagc gatggacttt tcagcgcctc ataaagaccg gaccgtgagt aaacctgagt
    13021 cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt agcgaattga tcgctgccac
    13081 gctaaaagaa accataattg ggcatatcgc catcgtggca ataccgagac gtgcggagaa
    13141 catttccgga atggagaacg gcgaaccgac cgagatatca atcaccgaat ttgccaccag
    13201 tacgatttcc accgcaccgg tgacaaaggt caacagacat gttacctgcg gcgtatagcg
    13261 cactgcacac cagattaatg caggcagcgg gaacgccaga ctccccgctc cgccaatgac
    13321 taccgaggca ataaccgaca caataagcgc gatggcgggc atcatctgtt ctgctttaaa
    13381 gcgcggcagc actccaggaa tagccaacgt cagcatacaa ggcacgatca acacgcccgt
    13441 tgagaattgc tcactgaacc agtccgcaag caaaggccag aaatccagac tatcaatact
    13501 gaccgaacca atcgccccga caatagcgca taataatgcg gcaatcagac agtaattaaa
    13561 tagccgtaag gcacttactg gctcatactt atttttcccc agacgcttat cccgcacgac
    13621 cagtaaggcg acggtaacaa taaacatcat attggagaaa ttgataacca gtgaaaccag
    13681 tccccattcg gtggttatgg catcataaac cagcatcgcc acataactga tcgcatagta
    13741 atgcagtcga ttaagccaga catagcgggc aaacaccccc gccattacgc cattcagcgg
    13801 ccagaaaaga gaaagttcgt gaaccagccg taactctgca ccaataaaat agaacagcgt
    13861 ggttagcgca aaaatagcga tgctattacg tagcagatga ccttcctgga acaaacggaa
    13921 agttgaaatg gggtgtacat gcattaatta tctgtccata caattgcggg tggcaattat
    13981 ctcaacgtaa taagtaaatt ctcataaagg atagcatgtt cgctaacagc ggcggcaaat
    14041 aaaggtaccc tgggaatgct attttatcgt tttccgcaat tctcatggct gattcatctg
    14101 tacaaccagc cagtgataga ttaccgccac gatataacgt tgttgttctt cgcttaacga
    14161 aacaccctgc ggaatgttat gccacttagc cagacaaccg cgacagcagg tcgcggtggc
    14221 gtgctgagcg ataaacaccg gatgcccgcg catgggggtt tgcttaccgt cattagccgg
    14281 taatgccgga gcaagacgtt tagcgacaaa atccgccgca tgttgttcga tgactggcgc
    14341 gcctttctcc aggcaatact gacgctcttt catgcccaga cgaaagcgag agcgaaattt
    14401 tgaacgggat aaacgcgcga agagtggatc gagagactgc attaataaac cgaacgccct
    14461 aactgtttcg tcaattgttc aaggacggca atacccgcaa gcgagttacc tgcatcatcc
    14521 agttccggac tccagacagc gatggccatt tcatgcggaa caatcgccac aataccgcca
    14581 ccaacgccag atttcgccgg tagccccacc cgccaggcaa actcccccgc gttctggtac
    14641 ataccactgg tcgccatcag cgcgttaatt tgccgcgcct gcattggcgt caccacaggt
    14701 tcatcaatat gaatagcttt cccctgatta gccagaaaga caaacgtcct ggccagctct
    14761 acacagctca ttttcagagc gcagtaatga aagtagtttt gcagaacggt tgtcacatca
    14821 tgatggaaat tgccaaacga cttcatcagc caggcgatag ccgcatttcg cgcggaatgt
    14881 tcaaattcgg aacgcgctac caccgtatcg tatgaaatat cagacacacc gcttaagccg
    14941 cgcacgactt ccagcatacg ttgccgtggt gcgcttaatc gcccttgcaa catatcgcaa
    15001 accaccagcg caccagcatt aataaacgga ttacgcggaa taccctgctc catttccagt
    15061 tgcactaaag aattgaacgg tgatccagac ggatctttgc cgacgcgttg ccagatttcc
    15121 tcttcggagt aatgacgcat ggcgacaacg agactcagca ctttggaaat agactgaatg
    15181 gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc cgtcaacggt acagatagca
    15241 atgcccaatc gggaaccgtc tactgtagcc agcgccggaa tataatccgc gactttaccc
    15301 tgaccaatga gcggccgcac ttgccgcaag atgttttcta aaattgcatt atccatggcg
    15361 actgccactt tctactcctg gaccgcaggt ctgaaaagac ctgcgagtat atcagagctg
    15421 aatatgtcgc gtcagatccg gtctttccac accgtctgga tattacagaa ttcgtgtaag
    15481 ccgaaatggg aaagctcacg accaaagcca ctctttttca cgccaccaaa ggccactcgc
    15541 gcgtcgctgg cacaataacc attgataaac accccaccgc attccagacg tgccgccatc
    15601 tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg aaaggccgaa ctcactatca
    15661 ttagccagtt ccagcgcatg ttctgcatct ttcgcaacgg tgattgccgc aacagggcca
    15721 aacatttctt cccgaaacgc ggtcatttct ggggtaacat tcgccagaac cgttggcgga
    15781 tagtagttac ctgccccagc catcttttcc ccgcccagta acaaacgcgc accctgcgcc
    15841 agggttttct ccacctgatg atgcagctca tcacgtaaat caaaacgagc cattggtccg
    15901 agagcgttct cttcatcacg gggatcgccc attttcaagg ctgccgcagc tgccacaaaa
    15961 cgttcggtaa atgcagaagc aattccctct tcgataataa agcgttttgc cgctgcacaa
    16021 acctgtcctg tattctgata acgtccggct accgccgctt taaccgccag ttccagatcg
    16081 gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt ccagtacgca ttttttcagt
    16141 gccgctccag cctgtgcgcc aatagccgct cccgcacgaa cacttccggt caccgtgaca
    16201 gcagcaatgc gcgaatcttt aatcatttga ctgacaccgt cgttgtcggc attcagccag
    16261 ccatatacgc cttgcgggat tcccgcatct ttaaacacct gggcaatgag ctgagcacag
    16321 cccatcacat tcggcgcatg tttaagtaag tagccgttac ctgcaagaat gatgggaacc
    16381 gcgccacgca tcacctgcca taacggaaag ttccacggca taatcgccag aatcgtcccc
    16441 aacggtcgat actcaataac tgcctgctga ttttccacca gcgtaggttc cgccttcagc
    16501 attgccggac catgttctgc ataccagtca cacaaattcg ccgatttcgc cacttcagcg
    16561 cgcgcctgat tgattggttt gcccatttca cgggtgatca tttgcgccat ttcttcgcta
    16621 cgggcgcgca gagccttacc gataccacgc agtttttcag cacgataatc tatatttgtc
    16681 tcgcgccagt cgcgaaagcc tgctgccgcc agctgaagtg cgttttcgat atcgttagcg
    16741 ccagcccacg gcagcacaga aagttgttca cccgtggcag gatttatcga aattgcatga
    16801 gttgccggag taatggtcat cggggtatct cctttatgag tcatggtatg aagatacgca
    16861 gatttactct tgctttaaaa tgaataatat taagccactt attcacgaat cgagaatatt
    16921 atggatctga cccaactgga gatgttcaac gcggttgccg aagctggcag cataacccag
    16981 gcagcagcaa tagtgcatcg cgtgccgtcg aatctcacta cccgtctacg ccagctggaa
    17041 acagaactgg gggtagatct gtttattcgc gagaatcagc gtttacgtct ctctcctgcc
    17101 gggcataact ttttacgcta tagccaacaa attctcgcgt tagtggatga agcgcggagc
    17161 gttgtcgctg gcgatgagcc gcaaggttta ttttctcttg gttcgctgga aagcaccgca
    17221 gcagtgcgca ttccggccac gctggcggaa ttcaaccatc gttatcccaa aattcagttt
    17281 tcactttcca ccggcccttc cggcactatg ctggaaggag tgctggaggg aaaactgaat
    17341 gcggcattta ttgatgggcc cattaaccat actgccattg acgggatacc ggtataccgc
    17401 gaggagctga tgatcgtcac gccacaaggt catgcgccag taatccgcgc cagtcaggtt
    17461 aacggcagca acatttatgc cttccgcgcc aattgttcgt atcgtcgcca cttcgagagc
    17521 tggtttcatg ctgacggtgc cgctccggga actattcacg agatggagtc gtatcacggg
    17581 atgttggcct gtgtggtcgc aggagcaggc attgcgctta ttccacgctc tatgctggaa
    17641 agtatgccgg ggcatcacca ggttgaagcg tggccgttag ctgagcaatg gcgttggtta
    17701 acaacctggc tggtctggcg tcgtggtgcg aaaacgcgtc cgcttgaggc atttattcaa
    17761 ctactggatg tgcctgactc ggcaaaacag ggttaccaat gagctatttt tgatagttct
    17821 tgcagctcaa atgccctata tagtgatgtt ccgatgactt atgactatat ggggcaaata
    17881 tggttacgcc agtaagcatc agtaattaca tatctcttcc tgatgatttt cccgtacgta
    17941 atattgcacc tcaggtaaaa gaagttttaa aggattttat tgatgcactc agtaccataa
    18001 tatgtgatga agagtggcgc acgtctttaa acaccaactc agccacaaaa aagatattta
    18061 acaaccttga taacttatca tatattcaga ggacttcttt tcggggtaac gacacactat
    18121 acaatgaaaa ggttcaattt aaactcactt attccgtcaa gaatggaaga cacaaagaaa
    18181 atattgaatt tcaggtagta ataaatttaa gccctattta tttagataat tttcgccatg
    18241 atggagaaat taatattttt gcgctcccca cccaaagcct gtcactatgg ggcgcgtatt
    18301 tcagaccggc gtcgagcgtg tgctatttct gtttctgaat gattttattg aacaatttcc
    18361 aatgatcaac cttggcgttc ccatcaaaag agcgcataca ccacatattg aacccctgcc
    18421 tccggatcgc cataccgctg cagattactt acgccagttt gatttgcttg tcctgaattt
    18481 tatctctcgc ggtaattttg tcatactccc ccgattatgg agtaactctg aggttcacag
    18541 atggttcgtc aataaagatc ctaacttgat caccgccatt ctcgacataa cagacggcga
    18601 attaaaagag gatttattgc aaagcctgat ggattcatta ggttctaaca aacatgtaca
    18661 acccgaagtc tgcatctgct ttttatccct tttagcagaa caagaatcac ctcattttca
    18721 ggactttttt ttatttttcg ccaatatgtt attgcactat caccaattta tgaatcccaa
    18781 tgaaagtgat ctgaatgacg tgttgatgcc agcatcatta agtgatgata aaattatcaa
    18841 acatatggcg cgcaggaccc tcaaactgtt tgtaaaaaat gaaacgccgc caaaagttac
    18901 tcacgaagac ctggtgaaaa acaggcctcg ctcccctgtc agaccaccta tacccgcaac
    18961 cgccaaaacg ccagacctcc ctgaacgtca ttaaaccgtg atgttaccga ctctctgacg
    19021 cggaacagaa tcagcgtcag agaaacggaa aacgcgatcc agatcacaaa tgcattgtat
    19081 tcacatcatt aaccgtttta agatcatttc atcacttttt cgcaactcac ccgataatct
    19141 gttatgacaa caaacactgt ttcccgcaaa gtggcgtggc tacgggtcgt tacgctggca
    19201 gtcgccgcct tcatcttcaa caccaccgaa tttgtccctg ttggcctgct ctctgacatt
    19261 gcgcaaagtt ttcacatgca aaccgctcag gtcggcatca tgttgaccat ttacgcatgg
    19321 gtagtagcgc taatgtcatt gccttttatg ttaatgacca gtcaggttga acggcgcaaa
    19381 ttactgatct gcctgtttgt ggtgtttatt gccagccacg tactgtcgtt tttgtcgtgg
    19441 agctttaccg ttctggtgat cagtcgcatt ggtgtggctt ttgcacatgc gattttctgg
    19501 tcgattacgg catctctggc gatccgtatg gctccggccg ggaagcgagc acaggcattg
    19561 agtttaattg ccaccggtac agcactggcg atggtcttag gtttacctct cgggcgcatt
    19621 gtgggccagt atttcggttg gcgaatgacc ttcttcgcga ttggtattgg ggcgcttatc
    19681 acccttttgt gcctgattaa gttacttccc ttactgccca gtgagcattc cggttcactg
    19741 aaaagcctcc cgctattgtt ccgccgcccg gcattgatga gcatttattt gttaactgtg
    19801 gtggttgtca ccgcccatta cacggcatac agctatatcg agccttttgt acaaaacatt
    19861 gcgggattca gcgccaactt tgccacggca ttactgttat tactcggtgg ttcgggcatt
    19921 attggcagcg tgattttcgg taaactgggt aatcagtatg cgtctgcgtt ggtgagtacg
    19981 gcgattgcgc tgttgctggt gtgcctggca ttgctgttac ctgcggcgaa cagtgaaata
    20041 cacctcgggg tgctgagtat tttctggggg atcgcgatga tgatcatcgg gcttggtatg
    20101 caggttaaag tgctggcgct ggcaccagat gctaccgacg tcgcgatggc gctattctcc
    20161 ggcatattta atattggaat cggggcgggt gcgttggtag gtaatcaggt gagtttgcac
    20221 tggtcaatgt cgatgattgg ttatgtgggc gcggtgcctg cttttgccgc gttaatttgg
    20281 tcaatcatta tatttcgccg ctggccagtg acactcgaag aacagacgca atagttgaaa
    20341 ggcccattcg ggcctttttt aatggtacgt tttaatgatt tccaggatgc cgttaataat
    20401 aaactgcaca cccatacata ccagcaggaa tcccatcaga cgggagatcg cttcaatgcc
    20461 acccttgccc accagccgca taattgcgcc ggagctgcgt aggcttcccc acaaaataac
    20521 cgccaccagg aaaaagatca gcggcggcgc aaccatcagt acccaatcag cgaaggttga
    20581 actctgacgc actgtggacg ccgagctaat aatcatcgct atggttcccg gaccagcggt
    20641 acttggcatt gctagcggca caaaggcaat attggcactg ggttcatctt ccagctcttc
    20701 cgacttgctt ttcgcctccg gtgaatcaat cgctttctgt tgcggaaaga gcatccgaaa
    20761 accgataaac gcgacgatta agccgcctgc aattcgcaga ccgggaatcg aaatgccaaa
    20821 tgtatccatc accagttgcc cggcgtaata cgccaccatc atgatggcaa atacgtacac
    20881 cgaggccatc aacgactgac gattacgttc ggcactgttc atgttgcctg ccaggccaag
    20941 aaataacgcg acagttgtta atgggttagc taacggcagt aacaccacca gccccaggcc
    21001 aattgcttta aacaaatcta acattggtgg ttgttatcct gtgtatctgg gttatcagcg
    21061 aaaagtataa ggggtaaaca aggataaagt gtcactcttt agctaacctt gcatcgcatt
    21121 gaccaaaact tgaaccgatt tagcaaaacg tggcatcggt caattcattt atttgactta
    21181 tacttgcctg ggcaatatta tcccctgcaa ctaattactt gccagggcaa ctaatgtgaa
    21241 aagtaccagc gatctgttca atgaaattat tccattgggt cgcttaatcc atatggttaa
    21301 tcagaagaaa gatcgtctgc ttaacgagta tctgtctccg ctggatatta ccgcggcaca
    21361 gtttaaggtg ctctgctcta tccgctgcgc ggcgtgtatt actccggttg aactgaaaaa
    21421 agtgttgtct gtcgaccttg gagcactgac ccgtatgctg gatcgcctgg tctgtaaagg
    21481 ctgggttgaa aggttgccga acccgaatga taagcgcggc gtactggtaa aacttaccac
    21541 cagcggcgcg gcaatatgcg aacaatgcca tcaattagtt ggccaggacc tgcatcaaga
    21601 attaacaaaa aacctgacgg cggacgaagt ggcaacactt gagcatttgc ttaagaaagt
    21661 cctgccgtaa acaaaaaaga ggtatgacga tgtccagacg caatactgac gctattacca
    21721 ttcatagcat tttggactgg attgaggaca acctggaatc gccactgtca ctggagaaag
    21781 tgtcagagcg ttcaggttac tctaaatggc acctgcaacg gatgtttaaa aaagaaaccg
    21841 gtcattcatt aggccaatac atccgcagcc gtaagatgac ggaaatcgcg caaaagctga
    21901 aggaaagtaa cgagccgata ctctatctgg cagaacgata tggcttcgag tcgcaacaaa
    21961 ctctgacccg aaccttcaaa aattactttg atgttccgcc gcataaatac cggatgacca
    22021 atatgcaggg cgaatcgcgc tttttacatc cattaaatca ttacaacagc tagttgataa
    22081 cgtgacaacg tcactgaggc aatcatgaaa ccactttcat ccgcaatagc agctgcgctt
    22141 attctctttt ccgcgcaggg cgttgcggaa caaaccacgc agccagttgt tacttcttgt
    22201 gccaatgtcg tggttgttcc cccatcgcag gaacaaccac cgtttgattt aaatcacatg
    22261 ggtactggca gtgataagtc ggatgcgctc ggcgtgccct attataatca acacgctatg
    22321 tagttattgt caggctctga tcacagagcc tgacatctcc gtaatatctt cctcaagtct
    22381 caaatatttt cgccattgtt ggcgatgaga tcccgatacc agtaaaacga cgctttacgt
    22441 atccgttcca gcgttccctt cccttcgttg tctttatcga cataaatcat tccgtagcgc
    22501 tttttcattt cccctgttcc ggcagaaacc aggtcaatgc agccccacgg ggtgtagcca
    22561 attaagtcaa caccatcttc aactacggct tttttcattt cccgaatatg ggaagcaaag
    22621 taatcaatgc gatagtgatc gttcaccgtg ccgtcagctt gtctctggtc aaccgcacca
    22681 aatccatttt cgacaataaa cagcggcaac tggaaatgat cccagaacca gttgagtgaa
    22741 taacgtagcc ctgccggatc aatttgccat ccccattcgg atgtatcgat ataagggttg
    22801 ctgaccaaat ctcgtggttc aacataatcc agttgcgggt tatcgggcga aaattgtgtc
    22861 gtaaaagaca tgtagtagct aaagccgata aagtcgacac aaccactggc aagaatcgcg
    22921 ttatcttctg gtgtgatatc gaggttgaat cctttcctgg caaagtaatt cagcatatgt
    22981 tgcggataat atccacgagc atgaacatca gtaaaccagt aacgacgatg cattgctttc
    23041 gtggccatca tcatatcgtt gggtgcacac gtcagaggat agatgggaca catggcgatc
    23101 atacagccaa tgttaaattc aggattgatc gattttccag tctgtaccgc cagggcactg
    23161 gcaactaact cgtaatgtac cgcctggtac attatttgtt cgcgctcatt gatatcttcc
    23221 tctggcgaat acaagatacc ggaattagta aatggacaca ggctttcgct gaaattcacc
    23281 tgattattga tttcgttaaa cgtcatccag tactttactt tttctttata gcgcgtgaag
    23341 accgttgatg cgaagcggat aaaaaagtcg atcagtttac ggtttcgcca gccaccatat
    23401 tttgtcacca gatgataagg catctcaaaa tgtgaaagcg tcaccacagg ttccattccc
    23461 tgcttaaggc attcatcgaa cagatcatca taaaattgta aaccctcttc attcggctcc
    23521 tgttcgtcac cttgcggaaa gattcgtgtc caggcaatgg aagttcgaaa gcatttgaat
    23581 cccatctcgg caaataactg aatatctgtt ttatagcgat gataaaaatc aattgcttca
    23641 tgattgggat aattaagccc gtcgataacg ccttctgtca cttcacgcgg caccccgtga
    23701 gcgccagcag tcatcacatc agcgatgctg atgccttttc ctccttcatt ccagccacct
    23761 tccaactgat gcgcggctac cgcaccgccc cataaaaaac cttttttaaa tcctgacata
    23821 cacaactcct taactaaaat agattaccac cagacttccg cctggatgcc ggccataaac
    23881 tcgtctttgc tgttctcatt aaacttaaaa ttcgataatt cgttatccag aatattgata
    23941 tacgttccat agaagcgaat ttctggtcgt gaacctaaaa tgctttcacc cactttcaat
    24001 gcatgccaga gtgtcgtttt ataagccgat tcatttagtg ttacaccctg ctgagttttg
    24061 ttctgttgct taaaccagcc taattcaagc cccgtctgat tccatgtatt ccagatccag
    24121 gccggtctta ttacagtgcg aatactgtca aaatcactat gagcaccact ttcataacta
    24181 taaacatctt cgccgtgaga atagacaagc gcgttagcca taataatatt gtcagtaaga
    24241 tacatctcgc cctgagagat taaacgccag gcgatcccat tggtatgatc accatagtaa
    24301 tagcgaccat gcgccatcgt attactggca tctgagaaac tggcaaaact gctggcatag
    24361 gaattattgg caacctgtaa cgtaaattca ttaaacgtat cgcgttgcag gttttgccgt
    24421 aaaacagaag tcagcatcca gctatctttc atttcaaaat aactgttgtc attttcgtta
    24481 tcttgttgtt gatccgtttt attaggtgcg gaatatttag ccattaatga taatgtcgca
    24541 tcatcccata acgggatatt gcgataacga acatccacag aattggtatt catctggctg
    24601 gtacgcgaaa aatcacgaga gtaaacatcg acatcatcac ggcttaagga catatcaaac
    24661 agccctacac caagtgccca gttttcaatc cccacacccg cggcgacatc cgtggttaag
    24721 gttttccagt ccagcatctg gatctcatat tgcgggagtt tatgtttgcc gacccagaag
    24781 tctgcctctg gtgcgaaggg taaaaaacct cgcgttgtca ggtaaatatc gctgaactgc
    24841 atgatatttt cattggcact gtcaccaaac caggcatcgt tatactgctc acccacgttg
    24901 ccatcatagg taacgaccgc atttgccgtt ttaccgtcct gattataaac acgctgattt
    24961 aaggtcaggt caaaccaacc gctcatctca ttaccaaaac gtcccagaga acccgctgca
    25021 taagtttgtg gtgagccgtg attaccagta ccccagcctg agcggaagta cccctgatag
    25081 ctgaacccaa tatcatcctt tatatattta ctgatatctt tcagggtcac gctttcgata
    25141 gtggttttgt cgcctttact ttcaataacg gcaacttgct gcgatccatt tatagtatgc
    25201 gctgcggtaa acgagttctg ttcaccgttt tcatttttga tgttatcagc gacgggagat
    25261 gtcgctgata tttcagcaag cgaggctgat ttatatttgt tttccgtgat gcttttttgt
    25321 aaggtcgaaa gtcgggattt atatactccc agttcattct gtgttgcttt cagctcttgt
    25381 ttattttgcg acaattcatt ttcaagcaat tcaaggcgct gctctatggt taattttgtt
    25441 gccatcgcgt tcaatggaaa caatgctgta ataatagaaa aagacaaaag gctgacgtta
    25501 agcgtcttta tattcatgac ttatccttaa gagtataata atttcctcca gctatcgcac
    25561 tggaggaaaa aatcaggcat ggagttcttt tcttaatgaa ataatttccc tggctaaatc
    25621 ctggcataat attgcattca tcaaatgatc ctgtgcatga atcagaatta aattaacttt
    25681 tattttgcca cagccttcat cctgactaat taatgcggtc tgaatcttat gtgcctgaag
    25741 acaggcagat tcagaactgg ccattagctg agttgcgccc tgccagtctt tttgcccggc
    25801 acaacgtatg gcttccatcg cgtcagaacg tgcctgcccc gcgttgacca gtaactccat
    25861 aacaagttct tcatctgcaa acatcacaat ctccttcctg ggtaaatgga attatccgtt
    25921 agttgtcatg ttattgactt tgttggcggc aataacaaaa ggtaaataga tcattgtcgc
    25981 cacacctaaa cataaaaaac caaccagtaa agccagccag ttccccccgg ttccgaggaa
    26041 agcaattaaa ggtccgggcg tagtccaggg cacggcataa gcaaccggtg gaataatttc
    26101 catcgaaaca aagaagtaac caatggcaca gttaacaatg ggaaccataa taaacggcac
    26161 catcatgatg gggttaagta caataggaag gccaaacatt atcggttcat tgatattgaa
    26221 aataccgggg ataaatgaca tttttgccag atcacggtat tccgcacgct tagaagcgat
    26281 aaaaatagcc aacaataacc ccaacgtcat acctgaaccg ccgcagttgg cgaatgcatc
    26341 attaatagaa gtccaggtaa tcggatatgg cgcgccccag gtagtgccat gttgagcggc
    26401 ccaggagaga ttctccagat tagcctcaga aaaaacggtt tctcgaattg ccgatgtggt
    26461 attaggtccg tggatcccca gcacccacag gaaattaccc acagccccca ggataattac
    26521 cgcaaagata ttcgttccca tatgtttgag cggcgtctgg attaatgtgt aaataagatc
    26581 gtttagtcct gccggggata tcagtgtcag gcaataatta agtgcggaaa agaacaccat
    26641 gacaaaaaat attggcaata aaactttaaa tgaacgcgct accgcaggcg gtacagctgc
    26701 cggcatcgtt atggtgattt ttgggtttcg agctaagcga caaaatattt cactggtgat
    26761 caatgcaacg ataacagcaa caaacaaccc ctgcgcgccg agatatttgg tcgtcaggaa
    26821 tgcttgccca tctatcatct gatatggcgt ataaacaata aaaaatgctc ctattgccgt
    26881 taatccgcat aaaagatcat cttgctcata gctaatcgcc atattcctgg cgaccaggaa
    26941 agcaatcata attgacatga tatttacaga accattcatt accggagtaa aaatagcttg
    27001 tgctttttca aggttgggga aaatgctatt cagatgcagt aatccggcaa taaaaccgtc
    27061 tggcgataat atggcaaagt taattaatat aattaatgaa cttgccatga taattggaaa
    27121 cgtaaaaacg aatccatctc tcaccgctgc aacatgtttt tgtgagttta acttgatagc
    27181 aactggaaca agaaaacgtt ccattccacg ttcgaatgag gccattaatc ccattttttt
    27241 atacctgttg ttttggatat ttatacatcc caataaagca tgtatgccat atccatttta
    27301 tgatgtgtga aaagttagtt caggtaatag tttaataaaa ttagattctt actcttgcag
    27361 tagtttcatc gcaaattcaa gaactgcctg tccgttcatg gtgccgtagt ctttcatctc
    27421 gataacggct acaggcattt tcccctgtgc aacggcttca atctctggtt tctgaaagcg
    27481 aacttgcggg ccaagtaaaa caacgtcaac ttcgttattt ttaattttcc ctttagcctc
    27541 tgcaatcgcc aatgcggaaa tattaacttc aagtgaaata gcgttagcat gatcaatcat
    27601 acgtttaacc agcatactgg ttgacatgcc cgcagcgcaa actaacaata tttttttcat
    27661 attttccctt actggtatat aacagattac attgtttaaa tatcatatcc cgcagtagct
    27721 atacgtcctg gtacattgat cacagtaata cagtgataaa gttgttcaac atcagggtga
    27781 ttattaaacg actcggccat acgataaacc aatcaggact caactccttg ttttcaatgg
    27841 ctcttaaaag gtgcaaaagt gatttttcaa aagattgccc gcttacttaa atccgaaatc
    27901 aatggcaatt catggcatgt tggtgatttg ttgccgtcag aagcggaact cgctgttcgt
    27961 tataatgttt cgcgtaatac cctgcgtaag gcattatcct tgctggaagg cgaaggaatt
    28021 attcacagaa agcatggttc aggaacatac attcaaaaaa agaattttgt tgcacacatt
    28081 gatcacatga acagtttcag tgaaattgcg cataaaagcg gcaaagaggc agggagtcag
    28141 attatgaaat ttgaagtgca ggatgcttca cccactattg cgactgagct gaatttagtg
    28201 actggtgagc aggtttatta cataaagaga ctgcgattca ttgaggataa tgcagcacaa
    28261 ttagaagaaa cgtggatgtc agtagcacgt tttcctgatt taaccgtgtc acatatgcaa
    28321 aaatccaaat tttcatatat tgagaatgaa tgcgggatca aaatcattgg cacctttgaa
    28381 actttctccc ctacttttcc taccccagaa atcgccagta ttttacggat cagcccacgg
    28441 gatcccatac ttaaaattca gacccaggct gtggacagta actctattcc gctggattat
    28501 tcgttacttt acagcaatat tttcgagttc caggtaaagt actttttccc gcgataaaag
    28561 cagacgcaca gagtgctgac atcacattct gtgcgtatgg tttagcctct aacctttacc
    28621 gctttacgcc accgcaagcc aaatacattg atatacagcc cggtcataat gagcaccgca
    28681 cctaaaaatt gcagacccgt taagcgttca tccaacaata gtgccgcact tgccagtccg
    28741 actacgggca ccagtaacga taacggtgca acccgccagg tttcatagcg tcccagtaac
    28801 gtcccccaga tcccataacc aacaattgtc gccacaaacg ccagatacat cagcgacaag
    28861 atggtggtca tatcaatagt aaccagactg tgaatcatgg atgcggaacc atcgagaatc
    28921 agcgaggcaa caaagaaggg aatgattggg attaaagcgc tccagattac cagcgacatc
    28981 accgccggac gcgttgagtg cgacatgatc tttttattga agatgttgcc acacgcccaa
    29041 ctaaatgctg cagccagggt caacataaag ccgagcatcg ccacatgctg accgttcaga
    29101 ctatcttcga ttaacaccag tacgccaaaa atcgctaagg cgatccccgc caattgtttg
    29161 ccatgcaatc gctccccgaa agtaaacgcg ccaagcacga tagtaaaaaa cgcctgtgcc
    29221 tgtaacacca gcgaagccag tccagcaggc ataccgaagt taatggcaca aaaaagaaaa
    29281 gcaaactgcg caaaactgat ggttaatcca taccccagca gcaaattcag tggtactttc
    29341 ggtcgtgcga caaaaaagat agccggaaaa gcgaccagca taaagcgcaa accggccagc
    29401 atcagcggtg gcatgttatg aagccccact ttgatgacca caaaatttag cccccatacg
    29461 accactacca atagcgccaa caccccatct tttcgcgaca ttctaccgcc tctgaatttc
    29521 atcttttgta agcaatcaac ttagctgaat ttacttttct ttaacagttg attcgttagt
    29581 cgccggttac gacggcatta atgcgcaaat aagtcgctat acttcggatt tttgccatgc
    29641 tatttcttta catctctaaa acaaaacata acgaaacgca ctgccggaca gacaaatgaa
    29701 cttatcccta cgacgctcta ccagcgccct tcttgcctcg tcgttgttat taaccatcgg
    29761 acgcggcgct acgctgccat ttatgaccat ttacttgagt cgccagtaca gcctgagtgt
    29821 cgatctaatc ggttatgcga tgacaattgc gctcactatt ggcgtcgttt ttagcctcgg
    29881 ttttggtatc ctggcggata agttcgacaa gaaacgctat atgttactgg caattaccgc
    29941 cttcgccagc ggttttattg ccattccttt agtgaataac gtaaagctgg tggtgctctt
    30001 ttttgccctc attaactgcg cctattctgt ttttgctacc gtgctgaaag cctggtttgc
    30061 cgacaatctt tcgtccacca gcaaaacgaa aatcttctca atcaactaca ccatgctaaa
    30121 cattggctgg accatcggtc cgccgctcgg cacgctgttg gtgatgcaga gcatcaatct
    30181 gcccttctgg ctggcagcta tctgttccgc gtttcccatg cttttcattc aaatttgggt
    30241 aaagcgcagc gagaaaatca tcgccacgga aacaggcagt gtctggtcgc cgaaagtttt
    30301 attacaagat aaagcactgt tgtggtttac ctgctctggt tttctggctt cttttgtaag
    30361 cggcgcattt gcttcatgca tttcacaata tgtgatggtg attgctgatg gggattttgc
    30421 cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc atggtggtta cgttgcaata
    30481 ttccgtgggc cgccgactta acccggctaa catccgcgcg ctgatgacag caggcaccct
    30541 atgtttcgtc atcggtctgg tcggttttat tttttccggc aacagcctgc tattgtgggg
    30601 tatgtcagct gcggtattta ctgtcggtga aatcatttat gcgccgggcg agtatatgtt
    30661 gattgaccat attgcgccgc caggaatgaa agccagctat ttttccgccc agtctttagg
    30721 ctggcttggt gccgcgatta acccattagt gagtggcgta gtgctaacca gcctgccgcc
    30781 ttcctcgctg tttgtcatct tagcgttggt gatcattgct gcgtgggtgc tgatgttaaa
    30841 agggattcgt gcaagaccgt gggggcagcc cgcgctttgt tgatttaagt cgaacacaat
    30901 aaaaatttaa ttcagccttc gtttaggtta cctctgctaa tatctttctc attgagatga
    30961 aaattaaggt aagcgaggaa acacaccaca ccataaacgg aggcaaataa tgctgggtaa
    31021 tatgaatgtt tttatggccg tactgggaat aattttattt tctggttttc tggccgcgta
    31081 tttcagccac aaatgggatg actaatgaac ggagataatc cctcacctaa ccggcccctt
    31141 gttacagttg tgtacaaggg gcctgatttt tatgacggcg aaaaaaaacc gccagtaaac
    31201 cggcggtgaa tgcttgcatg gatagatttg tgttttgctt ttacgctaac aggcattttc
    31261 ctgcactgat aacggatcgt tgacacagta gcatcagttt tctcaatgaa tgttaaacgg
    31321 agcttaaact cggttaatca cattttgttc atcaataaac atgcagcgat ttcttccggt
    31381 ttgcttaccc tcatacattg cccggtctgc tcttccaatg accacatcca gaggctcttc
    31441 aggaaatgcg cgactgacac ctgctgtcac ggtaatgttg atatgccctt cagaatgcgt
    31501 gatggcatgg ttatcgacta actggcaaat tctgacacct gcacgacatg cttcttcatc
    31561 attagccgct ttgacaataa tgataaattc ttcgcccccg tagcgataaa ccgtttcgta
    31621 atcacgcgtc caactggcta agtaagttgc cagggtgcgt aatactacat cgccgattaa
    31681 atgcccgtag gtatcattaa ccaatttaaa tcggtcaata tccaacaaca ttaaataaag
    31741 attcagaggc tcagtgttgc gtaactgatg atcaaaggat tcatcaagaa cccgacgccc
    31801 cggcaatccc gtcaaaacat ccatattgct acggatcgtc agcaaataaa ttttgtaatc
    31861 ggttaatgcc gcagtaaaag aaagcaaccc ctcctgaaag gcgtcgaaat gcgcgtcctg
    31921 ccagtgattt tcaacaatag ccagcattaa ttcccgacca cagttatgca tatgttgatg
    31981 ggcagaatcc attagccgaa cgtaaggtaa ttcatcgtta tcgagtggcc ccagatgatc
    32041 aatccaccga ccaaactggc acagtccata agaatggtta tccgttattt ctggcttact
    32101 ggcatctctc gcgaccacgc tgtgaaacat actcaccagc cactggtagt gggcatcgat
    32161 agccttattg agatttaaca agatggcatc aatttccgtt gtcttcttga tcattgccac
    32221 tcctttttca cagttccttg tgcgcgctat tctaacgaga gaaaagcaaa attacgtcaa
    32281 tattttcata gaaatccgaa gttatgagtc atctctgaga taacattgtg atttaaaaca
    32341 aaatcagcgg ataaaaaagt gtttaattct gtaaatcacc tctgcattat cgtaaataaa
    32401 aggatgacaa atagcataac ccaataccct aatggcccag tagttcaggc catcaggcta
    32461 atttattttt atttctgcaa atgagtgacc cgaacgacgg ccggcgcgct tttcttatcc
    32521 agactgccgc taatgttgat catctggtcc ggctgaactt ctcgtccatc aaagacggcc
    32581 gcaggaataa cgacattaat ttcaccgctc ttatcgcgaa aaacgtaacg gtcctctcct
    32641 ttgtgagaaa tcaaattacc gcgtagtgaa accgaagcgc catcgtgcat gttttttgcg
    32701 aaatcaacgg tcattttttt tgcatcatcg gttccgcgat agccatcttc tattgcatga
    32761 ggcggcggtg gcgctgcatc ctgttttaaa ccgccctggt catctgccaa cgcataaggc
    32821 atgacaagaa aacttgctaa tacaatggcc tgaaatttca tactaactcc ttaattgcgt
    32881 ttggtttgac ttattaagtc tggttgctat ttttataatt gccaaataag aatactgcca
    32941 attgttataa ggcatttaaa atcagcccac tagctgtcaa atattcagag aatttaactc
    33001 a
//
LOCUS       DAJOWB010000042        32964 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJOWB010000042 DAJOWB010000000
VERSION     DAJOWB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.042"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(97..963)
                     /locus_tag="OHO85_002177"
     CDS             complement(97..963)
                     /locus_tag="OHO85_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCQ2671546.1"
                     /translation="MVSRIVTDLRVPRMLLSVLTGAGLAMVGALLQTTTRNDLADPFL
                     FGLSSGASAGAVLVITRFGDRLGVLTLPVSAFVGGVCSAIAVMLLFHFIKQRGAEHLI
                     LCGLAISFLFGALTSYFIFSGDQRAASSVLFWSLGGLGLATWNNLPFAVFSLVLLFAF
                     VLLRWRSLDGVLAGEQTALSLGINVSRLRIEIFLCCALATSLMVALTGVIGFVGLMVP
                     HLCRHFAGVKHLLLLPLCGVWGAILLCGGDIVSRTILAPQELPIGIITAGIGGLFIIM
                     LLARNSSRNTAC"
     gene            complement(1116..2072)
                     /locus_tag="OHO85_002178"
     CDS             complement(1116..2072)
                     /locus_tag="OHO85_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCQ2671547.1"
                     /translation="MKKLFISLLAVLSASVASAADFPVTIESCGTPVTFAGPPKRAVI
                     NDLNMSEMAFALHLQDRIVGLTGISGWYKMTPEFKKAMGSIPELAPKYPTLETLLAAE
                     PDFFFAGWNYGMKVGGEVTPDTLSKYGIKTFVLSESCVFTTAHKNKATMDLLYNDVLT
                     LGKIFGKRNDAQSLVSGWKKRLNELPKPAAGTRPLKVFVYDSGEDKPFTSGKYAMPTA
                     IIEAAGGKNVMEAVDTSWGTTSWESVAATEPDFIILLDYQTGSGADALRHFLENHPLM
                     KLTPAVQHHRYLKLQYAELTPGPANVDAVEKLARAMYPSTAK"
     gene            complement(2094..2411)
                     /locus_tag="OHO85_002179"
                     /pseudo
     CDS             complement(2094..2411)
                     /locus_tag="OHO85_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851089.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2585..3847
                     /locus_tag="OHO85_002180"
     CDS             2585..3847
                     /locus_tag="OHO85_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2671548.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
     gene            complement(4041..5345)
                     /gene="ybtS"
                     /locus_tag="OHO85_002181"
     CDS             complement(4041..5345)
                     /gene="ybtS"
                     /locus_tag="OHO85_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     YbtS"
                     /protein_id="HCQ2671549.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCLARLHINADGTQATFIDDAGEQQWAVDSITDCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGGRRLCEWVKEASTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCLPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDMRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(5373..6653)
                     /gene="ybtX"
                     /locus_tag="OHO85_002182"
     CDS             complement(5373..6653)
                     /gene="ybtX"
                     /locus_tag="OHO85_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HCQ2671550.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LMIAAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRAGYGWG
                     NSVQVGGSYLGMMCGGGVFLMLSAASGWPVAMLMMAMLIMALSFPLWRITEPTRTAPI
                     PHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTFMMAPGHLLLPILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGVFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(6646..8448)
                     /gene="ybtQ"
                     /locus_tag="OHO85_002183"
     CDS             complement(6646..8448)
                     /gene="ybtQ"
                     /locus_tag="OHO85_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HCQ2671551.1"
                     /translation="MKDNNPADNLAWRVNWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIERFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVHNRTVIIIAHRLSTIAGAGNILVIEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARAWRDDGVSASGEWVHE"
     gene            complement(8435..10147)
                     /gene="ybtP"
                     /locus_tag="OHO85_002184"
     CDS             complement(8435..10147)
                     /gene="ybtP"
                     /locus_tag="OHO85_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HCQ2671552.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            10404..11363
                     /gene="ybtA"
                     /locus_tag="OHO85_002185"
     CDS             10404..11363
                     /gene="ybtA"
                     /locus_tag="OHO85_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HCQ2671553.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHGGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRVM"
     gene            11554..17661
                     /gene="irp2"
                     /locus_tag="OHO85_002186"
     CDS             11554..17661
                     /gene="irp2"
                     /locus_tag="OHO85_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HCQ2671554.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQKLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNHHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDDARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLHPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     VLAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLAGVKQATVLAIGEKEKTLAAYVVPQGEAFCITDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPIRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     AFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     IVRDGQQQVLEQTPPWVIPAHTLHTPEEALRVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPEFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQQQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEQSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTKRPRPVRRRQRRI"
     gene            17749..27240
                     /gene="irp1"
                     /locus_tag="OHO85_002187"
     CDS             17749..27240
                     /gene="irp1"
                     /locus_tag="OHO85_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HCQ2671555.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWAGEKSGALVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPERFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDQPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENPPWETLHQGLSAVSLSQRWLAARGDTLWLPSLAPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFNLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIAGHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAIVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGRCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDTPTEPAKPTPVAGGNPA
                     LEKQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFSHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQ
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGEQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTAGMV
                     SQNLTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPARWTPAETEWQGW
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSMQPENTL"
     gene            27237..28337
                     /gene="ybtU"
                     /locus_tag="OHO85_002188"
     CDS             27237..28337
                     /gene="ybtU"
                     /locus_tag="OHO85_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HCQ2671556.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITKMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSHLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            28334..29137
                     /gene="ybtT"
                     /locus_tag="OHO85_002189"
     CDS             28334..29137
                     /gene="ybtT"
                     /locus_tag="OHO85_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HCQ2671557.1"
                     /translation="MTQSAICIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTALQKQPSTSER"
     gene            29141..30718
                     /gene="ybtE"
                     /locus_tag="OHO85_002190"
     CDS             29141..30718
                     /gene="ybtE"
                     /locus_tag="OHO85_002190"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HCQ2671558.1"
                     /translation="MNSSFKSLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETVSDDFTPFFSLHGER
                     QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            30849..32870
                     /gene="fyuA"
                     /locus_tag="OHO85_002191"
     CDS             30849..32870
                     /gene="fyuA"
                     /locus_tag="OHO85_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HCQ2671559.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
BASE COUNT         7163 a         9988 c         9075 g         6738 t
ORIGIN      
        1 aaggattgtc ggttgctttc ataattggcg tcaacaacct caaccttcag aatgaataaa
       61 agacatcaga gccttatttt tctgtcttat atgtgcttaa caggccgtat ttctggacga
      121 atttcgtgcg agtaacatga taataaacaa ccctccaatg cccgccgtaa taatgccaat
      181 tggtagttcc tgcggtgcca gtatcgtccg gctgactata tcgccgccac acagtaatat
      241 tgcaccccag acgccacaca aaggtaacaa caacagatgc ttaactcctg caaaatgccg
      301 acacagatga ggcaccatta agccaacaaa tccaatcacg ccggttaatg ccaccatcaa
      361 cgatgttgcc agtgcgcaac aaagaaaaat ctcgatgcgc agacgactca cgttaatccc
      421 tagcgacaac gctgtctgtt ctcctgccag cacaccatcc agagagcgcc accgtaacag
      481 gacaaaagca aacagcaaca ccaggctgaa cactgcgaaa ggcaaattat tccaggtggc
      541 taatcccaga ccacccagtg accagaacag cacagagctg gcagctcgct gatcgcccga
      601 aaaaatgaaa taactggtca gcgccccgaa caaaaaagag atggcaagac cgcagagaat
      661 tagatgctca gctccgcgtt gctttataaa atgaaacaac agcattactg ctatggcgga
      721 acacacacca ccaacaaacg ccgatacggg gagcgttaat acaccaagtc gatcaccaaa
      781 acgggtgatc accagcaccg cccccgctga cgcgccggag gatagcccga acaaaaaagg
      841 atcggccaga tcgttacggg tggtagtttg taacaacgcc cctaccattg ccagtcccgc
      901 ccctgtcagc acagagagca acatcctcgg tacgcgtaaa tccgtcacaa ttctgctgac
      961 catggcagat accggctcat ccaccagctt cagtgctccc agcacctaca ttagcgacag
     1021 tgaaactgtc cctctggtaa tactgaataa catcaaccca atcagcacta ccatggctcc
     1081 ccacatcgcc cagtgataac ggtacgacat gagcatcact tcgccgttga cgggtacatc
     1141 gcccgcgcga gtttttccac cgcatcaacg ttggcaggcc ccggtgtcag ttcagcatat
     1201 tgtaatttca ggtatcgatg atgctgaaca gccggagtca gtttcattaa cggatgattc
     1261 tcaagaaaat gacgcagtgc atccgcaccg ctgcccgtct ggtaatccaa caaaataatg
     1321 aagtccggct cagtagcggc cacgctttcc caggaagtcg ttccccaact ggtatccacc
     1381 gcctccatta catttttacc accagccgcc tcaattatgg ccgtaggcat cgcatattta
     1441 ccactggtaa acggtttatc ctcgcctgag tcgtagacga acacctttaa aggacgggta
     1501 cctgccgctg gcttcggcag ttcattcaac ctctttttcc agccgctgac cagggactgt
     1561 gcgtcattgc gcttgccaaa aatcttaccc agcgtgagga catcgttata cagcagatcc
     1621 atggtggctt tatttttgtg cgcagtggtg aacacacagc tttcactcag aacaaaagtc
     1681 ttgatgccat atttgcttag cgtatccggc gtcacttcgc cacccacctt catcccatag
     1741 ttccagccag cgaaaaagaa atcaggttcg gctgccagca atgtttccag agtgggatat
     1801 ttgggtgcca gttctgggat cgagcccatc gcctttttaa attccggcgt cattttgtac
     1861 cagccactaa taccagtcag cccaacaata cgatcctgta aatgcagtgc aaacgccatt
     1921 tctgacatat taaggtcgtt gataacagcc cttttcggcg gaccggcgaa tgtgacagga
     1981 gtcccacaac tttcaatagt aacaggaaaa tcagcagcac tggctactga ggcagacagt
     2041 accgccagca gggatataaa caattttttc atcactgact ctcagaaagt aatttatgca
     2101 caatgcggaa cttcaaaaat acgcaacgcc tttcctgtat ggggatgcgg tacggtaaaa
     2161 ctggtcaagc caaagatggg ataaaggtat tctgatacca ataccctctc cggtgtgtca
     2221 cagatcactg attgcccctg cgacaagagg agaacacggt cagcaaatga ttctgtcagt
     2281 gtcagatcgt gcaataccgc cacgacggta attcccttgg tttttacaag ggaaagtagt
     2341 tcctcgcgcc cgggaggatc gagatgattg gtgggttcat caagcaatac caggtcgggc
     2401 gtctgtgcca gtcagaggag ccaattttct gttttcatgc ttccttgcga atccttatct
     2461 gatgttgatt caacaggtta gtgtgaaaac tcttcccggt gcattttgat tttaccctac
     2521 gcatcgggaa aaattggtgg tcaaatctgg ggtcaggtta gttcgataat ggagtgaccc
     2581 ccatatgtcc cttaccgacg caaaaatccg caccctcaag ccttctgata aaccctttaa
     2641 agtctccgat tcccacggtc tgtatctgct ggtcaagccg ggtggctccc gccactggta
     2701 tctcaaatac cgtattagcg gtaaagaatc ccgcattgcg ctgggtgcct atccagccat
     2761 ctccctgtct gatgcgcgac agcaacgtga aggtatccgt aaaatgctgg cgctgaatat
     2821 caacccggta cagcagcggg ctgctgaacg tggctcacga acaccggaga aagtttttaa
     2881 aaacgtggcg ctggcgtggc ataaaagtaa caggaaatgg tcgcagaaca ccgccgaccg
     2941 tctgcttgcc agcctgaaca atcacatctt tccggtcatc gggaacctac ctgtatcaga
     3001 acttaaaccc cgtcatttca ttgacctgct gaaagggatc gaggaaaaag gtctgctgga
     3061 ggttgcgtcc cgcacacggc agcacctgag taacataatg cgccatgcgg tccatcagga
     3121 gttaatcgat acgaaccctg cagcaaacct tggcggcgtg accacacctc ctgtcagacg
     3181 gcactatcct gccctgccgc tggagcggct gcctgaactg cttgaacgta ttggggcata
     3241 tcatcagggc cgtgaactga cccggcatgc cgttctgctg atgctgcatg tgttcattcg
     3301 ctccagtgaa ctgcgtttcg cccgctggtc agagattgat ttcacaaacc gagtctggac
     3361 gatacccgcg acgcgagaac ccattattgg cgtgcgttat tccggccgcg gggcaaaaat
     3421 gcgaatgccg catatcgtcc ccctctcaga acagtccatc gccattctga aacagattaa
     3481 ggatatcacc ggtaataatg aactgatctt ccccggcgac cataacccgt ataagccaat
     3541 gtgtgaaaac acggtcaata aggcactgcg ggtgatgggt tacgacacga aaaaggatat
     3601 ctgcggtcac ggcttccggg caatggcatg cagtgcgctg atggaatcgg gtttatgggc
     3661 aaaggacgca gtagaacgcc agatgagtca tcaggagcgc aataccgtgc gcatggctta
     3721 tattcataag gcagagcacc tagaagcccg caaagcgatg atgcagtggt ggtcggatta
     3781 tctggaagca tgccgagaat cttatgcacc gccttataca attggtaaaa ataagtttat
     3841 cccatagtat actaacgaat cccacactct ggcaaattac caccacctct acaggaggtg
     3901 gttctatata gacttttatc aacttcatga cgaaaaacac ctattttatc caaaaagaag
     3961 acaatagagt cacagaggaa tcaaagagcg actcaattgt ctttggacag tctggttgtg
     4021 aggttagaaa acagttactc ctacaccatt aaatagggcg caatgctcgc taatttctcc
     4081 cgggtttccg tcagttcgcg ttccggtgta gattgcgcga tgattcccgc ccccgcttgt
     4141 atccagcagc gctggctgtc ctgaaatacg gaacgcagaa ctagcgccgc atcgaagcgc
     4201 atatcgtcca gcagcagaat tgcgccggaa tagagctctc gcggcgtctt ctcaatttgc
     4261 ataatcgcgt tcagggcagc gtttttaggg atacctgagg cggtaatcga cggaaacagc
     4321 acggtaaacg catcccaggc atccttgttt tccgcaagct gaccgctcac gccggacccc
     4381 agatgctgaa cgctgccgcg ctggcgaacc gacattaaat cttcaaccac cacgctgccc
     4441 ggcaggcaaa cggcctccag ttcagcaatc gcttctttga cagaaaggat atgctcaagc
     4501 acctctttac tgtcgtgcag cagttctgcc tctttcgcct tattatgctc cgggtttccc
     4561 atgcgatcgc gggtgcccgc aagcggttca gtgaccactt tattgcccgt cactgacatc
     4621 accagttccg ggctaaagcc cagcgcttcg cgtccttcct gacggaacat aaacgagcgc
     4681 acaggtgtat ttgcctgccg cccgtataac agcgtggcgg gcatatcaat ccgcgatggc
     4741 aggggaatag cgcgcgagac aatcactttg acatactcgc cacggcggat ctccgcaacg
     4801 gcgcgtgcaa cctgttgttt atacgcctcg ccattgaggg cggtatccac cgccagtggt
     4861 gcgttctgcg tcgttgtact ggcctctttt acccactcac acaaacgtcg gcccccgtcg
     4921 gcggagtccg cataaacggt gacatttccc ttttcaaaaa taagttcttc acggggaacg
     4981 gttaacgtca gcagcggcca ctccccggcg ttaaaggcaa tcccccgcgc atgcgccgca
     5041 aagttgaacc caacctgtcc ataaacccga cgtcctttga cctgaggatg tgccataaaa
     5101 cgacgcgcgc agtcggtaat ggaatccacc gcccattgtt gctccccggc atcatcaata
     5161 aatgtcgcct gcgttccgtc ggcattaatg tgcaaccgtg ccaggcaccc tttgcctaaa
     5221 taccaacagg gttgacgctc atagacataa cactcttctt ctgcgaactg gcgtaaaacg
     5281 ccagaaatcg tcggcagcca ttgttcctct ggtaacgcca ggtgtaaaaa ttcactgatt
     5341 ttcatccctt acctctctgt gttattcccg gatcaatcaa ttgcgcttgt catcagttct
     5401 cttgccgaat gcagccggat ataagcaacc cacgccgcca gcaacgtgaa taccccggca
     5461 aacaggaagc aggccgcata gccaaaatgt tgagcaacaa cgccgccgat aacaccagcc
     5521 aggatggcga ttgccgcgtc agtacattga aagagggtga agtcgacacc ggcctgcaaa
     5581 ggcgatgaaa gcgacatcag cgtggcgtaa agacagacca gcgcgcaggc cagcgaaatg
     5641 gactggacaa tgaccagaca ctggagaatc ggcagcaaca gatgacccgg cgccatcatg
     5701 aacgtcatca ccaccgccag cagcgcgatc ccctggacgc cataagccgt caacagcgct
     5761 ctgccgggtg aggtgtattt catcagtaat ccgccggcca gcgttcctgc tatgcccgct
     5821 gcaatattgc cgccgctgaa cagcgcgccc aacgcagaca tgctcaaccc atgatccaac
     5881 aacagcggcg ccagaagagg cagcacaaac cgcatgcctg aattcagcat taataccagc
     5941 agtaagccca ggcgcgcctg cttcctcctt agcgcataac ctaacgccgg aacatgcggg
     6001 ataggcgctg ttcgcgtcgg ctccgtaatg cgccacagcg ggaatgacag cgccataatc
     6061 agcatcgcca tcatcagcat ggcgacaggc cagccggatg ctgccgacaa catcaggaac
     6121 accccgccgc cgcacatcat tcccagatag ctgccgccga cctgcacgct gtttccccag
     6181 ccgtaacccg cgcgagtcag ttgatcgacg caaaagccgt cgctggcgat atcaatggtg
     6241 gaagcgaccg tgcctgccac cataaacaga acaaataacg cgacgatttg cgtatcggga
     6301 aatcctcctt ctcgcccaaa ccagccaatc gctgcggcaa tcatcaggat cgccgctagc
     6361 gcgacctgac cacgcaggat taacatccgc gaacggcgtt cctggctacc gggcggaagc
     6421 cgccagcgtt cgatccacgg cgcccaaata aacttcagcg cccagggcag catgaacagg
     6481 gttgtcgcac cggcaagcgc cagcgatccg ccagcagcgc gcaccagcgc gggtaaggac
     6541 tgcatagaca gcgccgatac cagactctgc gtcacataga cgccggcaat agcaaaaatc
     6601 acccgcccgg tcgtcaacgt cagcggtttc acattcgact gaacatcact catgcaccca
     6661 ctctccagac gcggaaaccc cgtcgtcgcg ccacgcgcgc gcggccattt gcgcctgcca
     6721 cagcgcctga taacgtccat gatgtgagag caattgcgca tgagtaccct gctcaaccac
     6781 ctgtccctct tctatcacca gaatgtttcc ggccccggcg atggtggata aacggtgagc
     6841 gatgataatc accgtccggt tgtgaaccag gttatcgatc gctttttgca ccgccagctc
     6901 gctttcaata tccagcgcgg cagtcggttc atcgagaatg acgaccggcg cgtttttcaa
     6961 taacgctctg gcaatggaaa tccgctggcg ctcgccgccc gatagctggc cgcccatctc
     7021 tcccattggc gtcagccagc cttgcggaag acgggaaata aactcaaggc actgcgccgc
     7081 gcgggcggcc tcttctacct cctgccgcgt cgcctgtggg cgcgcgatac ggatattcgc
     7141 cagcagcgtg tcatcaaaca gccagacatc ctggaaaaca acagaaatca ggctattgag
     7201 ctgttccggc gtcaggcggc gaatatcgac gccgccaata gaaatctgcc cttgctgcgg
     7261 atcggcatag cgcattaaca gtttggtgac cgtagttttg cctgcgccgg aggcacccac
     7321 cagcgcgctc atactggctg ccgggaacgt caaagaaaca tgattaagcg cgtggccgtc
     7381 gccttcttcg tagcgatagc tgacgttgtc aaagcggata tcgtaacgtt ccggcatctc
     7441 gctttgctct gcgacgggaa gcggtgctat cgccataaac cgctcaatcc gttgcagggc
     7501 gctggcgatc agttccacaa ccgaggtgta gctgataaac atcgccatcg gttctgcgaa
     7561 gcgcataatc atcgcgacgg cggcaatcaa aaaggcgagg ttcagagtgc ccgtcactac
     7621 ccagacgatc ccggataaca ccaccacctg taggcccaac tccacgacgc tggcgatcag
     7681 catcgtcgcg ccagcgccct gacggtgagt gcgggtctgt aagttttcca gcgcattgaa
     7741 atgcgccagc agcgcccggc ttttatcggc atcgctgccg caggtgcgca ataccatcat
     7801 cccctgagca aattcaacga tatcgccgct cagacgctgg tgcgcttccc ccagcgtctg
     7861 catttgtcgc cgcatcgccg ggcggcgcca gtaataaaac ggcaccagca gagggaagat
     7921 cagcaacatc accagcccca gccgccagtc tatccacaat gtcgccagcg acgccgtcag
     7981 cggcgtgaca atggtgagca gcaaaatatt ggctatcgca ataacataat tgaggttttc
     8041 atccacgctg cccagcagca aggcgttcat ttcacccgcc ctgccgcgct ggagcttctc
     8101 cagcggcacg cggcgtaact gctcgccaag tcgcaggcgc aactcatggg tggcctgcgc
     8161 cagatgacca cggtattcaa agcccaggcc ataccagcgt agcaccagcg tcacaattgc
     8221 ggcgacgctg aaggccatag cccagttaag aagttgcggc gcgtctcccc gtaacagcgc
     8281 atcaatgatc ggataaagac aggcaaaggc gatcccctgc atgaatgccg ccagcaacag
     8341 cgccagcata ctgcgtcgca gcatcctggc ctgactgcca acgctggaga taagctggcg
     8401 ccagttgacg cgccaggcca ggttatccgc aggattattg tctttcattt tccaccacct
     8461 cttcctgtgt tccacccggc acccaatgct gcgcctgctg atagtgttgc cagagccgtt
     8521 gatacaggcc gccctgcgcc aacagttgcg tatggttccc catttccctg agctgtccgt
     8581 cggaaaacag caatatcaca tcggcctgag tcaccattga gagacgatgc gcgaccatga
     8641 tgaccgtccg gccacgcatg gccgccgcga gcgccttgat aagcgccgct tcgttttccg
     8701 ggtcagcaaa cgccgtcgcc tcatcgagca ccaggatggg gcgatcctgc aaaagcgccc
     8761 gggcgatagt aatgcgctgc cgctggccgc cggagagaaa taccccacgt tccccgactc
     8821 gtgtgttgta accttctggc agagcgctaa taaaatcatg ggcctgcgcc actctggccg
     8881 ccgctattac cgcctccagc ggcgtatccg gcgcgcccag acgaatgtta ttggctatcg
     8941 tgtcggcaaa aaggaagttg tcctgaaaca caaacgagag ttgcttcatc agggtgtccg
     9001 tctgcatatc acgcagatcc acgcctccaa tacggatatg gcctttgtcc gggtcggcgt
     9061 aacgcagcag caagcgcgcc acggtgcttt ttccggcgcc gcttggcccg accagcgcca
     9121 caatttgccc ggcaggcaca tgaaagctca cctcctgcaa cgcggcgcca gtacgcgctt
     9181 gcggataatg aaagctcacc tgctcaaagg taatgctcgc ctcttgcggc tgctggtcag
     9241 actgcggcag cgataactcc ggcatcgcga gaacctgata aatacgctga atgcttaaac
     9301 gcgtttgcgc gaccaggtta ttgagcatca tcattggcat tacggcttcg gccattccgc
     9361 tgcccagcag taaaacggcc acccacgcga taaaatcgaa actgccatag tgcagcaggc
     9421 cgtatcccga ccagatcagg acaaacaggg tcgggagagg attcagaatc gagaaggaaa
     9481 aacgcgctga aaaaccggct ttgcgatacc aggttttgag cacatcgacc cactcttcaa
     9541 gggcgcgttg atagcgcaaa aaactggtgc tgccgctatc aaacgttctc accacgggca
     9601 tggcctgcac gaactcaatc accgccgcgc taacctgctc gcgcgcctta tgataacgct
     9661 gagccatatc ttccgaacgc tggcgggcga gcacgagaac aaccgatcca aacgccagta
     9721 cccccagcgt cgcgattgcc agccgccagt ccagccagaa caagataacg attgtcgcca
     9781 gcggcatgat gatcgcgcgg gcatagagcg gcgtgctgtc ggccacaaaa atatgtaacg
     9841 acttcacgtc atccagcatc actttcgcca ctgacccgct gcccatttgc tgtaacacgc
     9901 caggcggaag ctgcaatgct ttacgggcta acctgctgcg cagaatgttc tccagatgaa
     9961 aagccgcgta atgagaggtg ttgaacgcct gtaagcgcag aacaaaagtt aagaccacgc
    10021 agccgatcac gccgcccagc ggccaggccg gaatagcgtc aggcgtcgcg cgaatgtcac
    10081 gcaggctcca cgccagaaaa aggagcgccc ccaggctggt taatgccgcc agccccgcca
    10141 gcgccatagc taaaataacc ttcctgcgaa ccggtgtaat cacctgccag agaggaaatc
    10201 gggacagcga ttcagtattg gatgattgag atgacatggg agtaactgaa tttcctgatg
    10261 aatttttacg gacgccaaaa acgacaattc agggtggcgt tctgagaatt aatgtgaata
    10321 ataaccatta tcaataactc atctacccca ttcggttcta tttatacccg cattggtcta
    10381 agccacaggg agataaccag gtcatgacgg agtcaccgca aacgcaatct gaaatctcta
    10441 ttcaccagtt ggtggtcggg aaacctgcaa acgatggcaa tattcctgct cagtgtgaac
    10501 tgctgcgctg ttcgttgcag gaaggtatgg atattttgct ctggaggggc cattttgcgc
    10561 ggcctgagac gttgcagcta cacgacgatc tgggcaggat taacttctcg tgcatcctgg
    10621 aaggcacttc gcgctttgcg attcagggac tccggcgcca caccgactgg gagcttgcgc
    10681 gcaatcgcca ttacattacc catacgccag actgtcgcgg cagcgcctcc tattgtggcc
    10741 gattcgagag cattaccctc tccttcagcc ctgaaaccct cgcgctgtgg gtgccggata
    10801 ttagcgcggt cattaagaat aagatcgact cccactgctg ttgccagcag cagcgttgta
    10861 atgctgaaac ccacttaacg gctcaggcct tgcgtcacgc gctgatgcgc atgcacggcg
    10921 gcttcagtca tgaacaaaag ccgtcgacgc tatggttgca ggggcaaagt ctggtcatgc
    10981 tcagtctggt gctggatgaa catcgcgaag acgccagttg cctctcctgc cacttcaacc
    11041 cgatggaacg ccagaaactg ctgcgggcga aggatctgct gctggctgat ctgacgcaag
    11101 cgccgggcgt cgcggagctg gccagagagt ccggtctgag cgttctcaaa attaagcgag
    11161 gcttccgcgt tctgttcaac aacagcgtct atggcctgtt tcaggctgaa cgaatgcagg
    11221 aagccagaag acgcctggcc aacggcaata cctccgtcat gacggtggcg gccgatctgg
    11281 gctatgccaa cgccagccat ttctctgccg ccttccagaa acagtttggc gtaacccctt
    11341 cgacctttaa acgcgtgatg taaccggggt cgcgcccccc taaactcctc cctgacagag
    11401 gcccggatac cccatccggg cctctgttct ttccgcctgt taccaacccg aaaggggcat
    11461 aaataaaccc gtttcgggta gcatccaatt gttaataatt attattctca tatgagcaat
    11521 gcttttcggt aagacgtgcc atcaggagga agaatgattt ctggcgcacc atctcaggat
    11581 tcgctgttac cggacaaccg ccacgcggct gattaccaac aattacgcga gcggctcata
    11641 caggaactga atttaacgcc gcagcagtta catgaagaga gcaacctgat ccaggccggc
    11701 ctggattcca taagattgat gagatggtta cactggtttc gtaaaaatgg ctaccgcctt
    11761 acccttcgcg agctgtatgc cgcccccacg ctggcggcat ggaaccagtt aatgctcagc
    11821 cggtcgcctg agaacgcgga agaagaaacg ccgcccgacg aatcatcctg gccgaacatg
    11881 accgaaagta cccccttccc attgacgcca gtacagcacg cctacctgac gggccgcatg
    11941 ccggggcaga agcttggcgg cgtgggttgc cacctgtatc aggagtttga aggccattgt
    12001 ctgacggcgt cgcagctgga gcaggccatc acgaccttgc tgcaacgcca cccaatgctg
    12061 catatcgcct ttcgccccga cgggcagcag gtctggctac cgcaacctta ctggaacggc
    12121 gtcaccgttc atgatttacg ccataacgac gctgaaagcc gccaggccta tctggacgca
    12181 ctgcgccagc gcctgagcca ccgtctttta cgcgtggaaa tcggcgaaac gtttgatttt
    12241 cagctgacgc tcttgccgga caatcaccac cgcctccatg tcaatattga cctgctgatt
    12301 atggatgcct ccagctttac gcttttcttc gatgagctta acgccctgct ggccggagaa
    12361 tcgctgccgg ctatcgacac ccgctatgat ttccgctcgt atttgctgca ccagcagaag
    12421 atcaatcaac cactgagaga cgacgcgcgc gcttactggc tggcgaaagc atcgacgctt
    12481 ccccccgcgc ccgtcttgcc gctggcctgc gaacccgcca cgctacgtga agtccgtaat
    12541 acccgacgcc gcatgattgt cccggcaaca cgctggcacg cctttagcaa ccgggccggc
    12601 gagtatggcg tgacgccgac aatggcgctg gcgacctgtt tttctgccgt gctggctcgc
    12661 tggggcggcc tgacgcgtct gctgcttaac atcaccttat tcgaccgcca gccgctgcac
    12721 ccggcggttg gcgcgatgct tgccgacttc accaatattc ttctgctgga taccgcctgc
    12781 gatggcgata ccgtcagcaa cctggcgcgt aaaaaccagc tcacgtttac ggaggactgg
    12841 gagcatcgcc actggtccgg cgtcgaatta ctccgtgaac tcaaacgcca gcagcgctac
    12901 ccccacggcg ccccggtggt atttaccagc aatctggggc gttctctcta cagcagccgc
    12961 gcagaatcgc cgttgggcga gccggaatgg ggcatctcgc aaacgccgca ggtctggata
    13021 gatcatctgg cgttcgagca tcacggcgag gtctggctac aatgggacag caacgacgcg
    13081 ctgttccctc cggcgttagt cgaaacattg ttcgacgcct actgccagtt gattaaccaa
    13141 ctctgcgatg acgaaagcgc ctggcaaaag ccgttcgcag atatgatgcc cgccagccag
    13201 cgcgcgatac gcgaacgggt caacgccacc ggcgccccca ttcccgaagg cttgctgcat
    13261 gaaggcattt tccgtatcgc tctgcaacag ccgcaggcgc tggcggtaac ggacatgcgt
    13321 tatcagtgga attatcatga gctgacagac tatgcccgcc gttgcgcggg caggttaatc
    13381 gagtgcgggg ttcagcccgg cgataatgtg gctatcacga tgtcgaaagg cgcaggacaa
    13441 cttgttgcgg ttctggccgt cctgctggcc ggggcggttt acgttccggt ttcgctggat
    13501 cagcctgccg cacggcgcga gaaaatctac gctgacgcca gcgtccggct ggtgctcatt
    13561 tgtcagcacg acgccagcgc cgggtcagac gatattcccg tccttgcctg gcagcaggcc
    13621 attgaggcgg agccgatcgc caacccggta gtacgcgccc ccacgcaacc ggcctacatt
    13681 atctacacct ccggctctac cggtacgccg aaaggggtag tcatttctca ccggggagcg
    13741 cttaacacct gttgcgatat caatacccgc tatcaggttg gcccgcatga cagggtgctg
    13801 gccctctccg ccctacattt tgatttatcg gtttacgaca tttttggcgt actgcgcgcg
    13861 ggcggcgcgc tggtgatggt gatggaaaat caacggcgcg atcctcacgc atggtgtgag
    13921 ctgatccagc gccatcaggt cacgctctgg aacagcgtcc cggcgctgtt cgatatgctg
    13981 ctgacctggt gtgaaggttt cgccgacgcc acgccggaaa acctgcgcgc agtgatgctt
    14041 tccggcgact ggatcgggct tgacctcccc gcccgttatc gggccttccg gccacaagga
    14101 caatttatcg cgatgggcgg cgccaccgag gcgtctatct ggtctaacgc ctgcgaaatt
    14161 cacgacgtcc ccgcccactg gcgttccatc ccttacggtt ttccgctaac caaccaacgc
    14221 taccgggtgg tggatgaaca gggccgggac tgccctgact gggtgccggg tgaattatgg
    14281 attggcggca ttggggtcgc ggaaggctat ttcaacgatc ccctgcgtag cgagcagcaa
    14341 tttttgacgc tcccggacga gcgctggtat cgcaccggcg atctcggctg ctactggcca
    14401 gatggcacaa tcgagttcct cggtcgtcgc gacaagcagg tcaaagtcgg aggatatcgc
    14461 atcgagctgg gcgaaatcga aagcgcgctc agccagctgg cgggggtgaa acaagcaacc
    14521 gttctggcga tcggcgaaaa agaaaaaacg ctggcggcat acgttgttcc tcaaggcgag
    14581 gctttttgca ttaccgatca tcggaacccg gcactgccgc aggcgtggca cacgcttgcg
    14641 ggaacgttgc cctgttgcgc tatctcgcca gagatctccg cagaacaggt agccgatttc
    14701 cttcagcatc gcctgctaaa actgaagccg ggtcacaccg ctggcgccga tcctctcccc
    14761 ctgatgaact cactcgctat ccagccgcgc tggcaggccg tggtggaacg ctggttagca
    14821 tttctggtga cacaacggcg actgaagccc gctgctgaag gttatcaggt ctgcgctggt
    14881 gaagaacgcg aggatgagca cccgcacttc agcggacatg atttaacgtt atcgcaaatt
    14941 cttcgcggtg cccgtaacga actgtcgtta ctgaacgacg cgcagtggtc gccggaaagc
    15001 ctggccttta accatccggc cagcgccccg tatattcagg aactggcgac aatttgccaa
    15061 cagcttgcac agcgcttaca gcgcccgata cgcctgcttg aggtgggaac ccgcactggc
    15121 cgcgccgcag aatcgctgtt agcacagctc aacgccggac agattgagta tgtcgggctt
    15181 gagcagagcc aggagatgct gctgagcgcc cggcagaggc tcgccccctg gcctggcgcc
    15241 cgtctgtccc tctggaatgc agacacgctg gcggcgcacg ctcactcggc ggacattatc
    15301 tggcttaata acgccctgca tcgtctgctg ccggaagatc ccgggctcct tgcgacatta
    15361 caacagcttg ccgttcccgg cgcgctgctc tacgtgatgg agtttcgcca gttaacgccg
    15421 tccgccctgc tcagcacgct cctgttaacc aatgggcagc cggaggcctt gctgcataac
    15481 agcgccgact gggcggcatt atttagcgcg gccgccttca actgtcagca tggcgatgag
    15541 gtcgcggggt tacaacgctt cctcgtacaa tgtcctgaca ggcaggtgcg ccgcgatccc
    15601 cgtcaacttc aggccgccct cgctggacgt ctgccggggt ggatggtgcc gcaacggatc
    15661 gtcttcctcg acgccttacc gctgacggct aatgggaaaa ttgactacca ggcgctgaag
    15721 cgtcgtcata cccctgaagc ggaaaacccg gccgaagcgg atttacccca gggcgacatt
    15781 gaaaaacagg ttgccgccct ctggcagcaa ctcttatcaa ctggcaatgt caccagagaa
    15841 accgacttct tccagcaagg cggcgatagc ctgctggcga cccgtctgac cgggcaactt
    15901 catcaggcag gttatgaagc gcaattaagc gacctgttta atcatccccg gctggcggat
    15961 tttgccgcca cgctgcggaa aaccgacgtc ccggtcgaac aaccattcgt ccactcccct
    16021 gaagatcgct accagccctt tgcgcttacc gacgtgcagc aggcttacct ggtggggcgt
    16081 cagccgggct ttgccctggg cggcgtcggc tcacatttct ttgttgaatt tgaaattgcc
    16141 gatctggacc tcacccggct ggagacggtc tggaaccgat taatcgcccg ccacgatatg
    16201 ctgcgcgcca tcgtgcgtga tggacagcaa caggtgctcg aacagacgcc cccctgggtg
    16261 atacccgcac acaccctcca tacgcctgaa gaggcgttgc gggtgcgcga aaaactggcg
    16321 catcaggtac tcaaccccga agtgtggccg gtattcgatc tccaggtcgg atacgtggac
    16381 gggatgcctg cccgcctgtg gctgtgtctg gataacctgt tgcttgacgg tctgagcatg
    16441 cagatcctgc tggcggagct ggagcacggc taccgctacc cgcaacagct gcttccgccg
    16501 ctgcccgtca ccttcaggga ttatctgcaa caaccctcgc tacagtcgcc caatccagat
    16561 tctctggcat ggtggcaggc gcagcttgat gatattcctc cggcgcctgc gttgccgctg
    16621 cgctgcttgc ctcaggaggt tgaaacaccg cgcttcgccc gcctgaacgg cgcgctggac
    16681 agcacgcgct ggcatcggct gaaaaaacgg gcggctgacg cccatctcac cccgtcggcc
    16741 gtactgttgt cggtgtggtc aacggttctc tctgcatgga gtgcacagcc tgagttcacg
    16801 cttaacctta cgcttttcga caggcgaccg ctgcacccgc aaatcaacca gattctgggc
    16861 gatttcacct cgctgatgct gctgagctgg catcccggcg aaagctggct gcacagcgcg
    16921 cagtcactac agcagcggct gagccagaac ctcaaccacc gcgatgtgtc agccatccgc
    16981 gtgatgcgtc aactggcgca acagcaaaac gtgcctgccg ttccgatgcc cgtcgtcttt
    17041 accagcgcgc tgggctttga gcaggataac ttcctcgccc ggcgtaatct gctcaaaccg
    17101 gtctggggca tctcccagac gccgcaggtc tggctcgatc accagattta tgaatccgaa
    17161 ggcgaactgc gctttaactg ggattttgtc gccgcgctgt ttcctgccgg gcaggtggag
    17221 cgccagtttg aacagtattg cgcattgcta aaccgaatgg ccgaggatga aagcggctgg
    17281 caactgccgc tcgccgcgct ggtgcctccc gttaaacacg cagggcaatg cgcagagcgc
    17341 tcaccgcgcg tatgccctga gcaatctcag ccacacattg cggcggacga gagcaccgtc
    17401 agcctgattt gcgacgcctt ccgcgaggtg gttggcgagt ctgtcacgcc cgcagaaaac
    17461 ttctttgagg cgggcgcaac gtcgctgaat ctggtgcaac tgcacgtttt gttacaacgt
    17521 cacgaatttt ccaccctgac gttgcttgac ctcttcaccc acccttctcc tgctgccctg
    17581 gccgattatc tggccggcgt cgccacggtg gagaaaacaa aacgacctcg ccctgttcgc
    17641 cgtcgtcagc ggcggatata gcgcgaagca aactgatttt ccccggaacg ccatcgcgaa
    17701 cgcatggcgt tccattgact ttgtgaatct taggaaacgg gaccgattat ggataacttg
    17761 cgcttctctt ctgcgccgac agcagattcc attgatgcat cgatcgctca acactacccg
    17821 gactgcgaac ctgtcgcggt tatcggctac gcctgccatt ttcctgaatc gccggatggc
    17881 gaaacgttct ggcaaaatct gctggaaggt cgtgaatgca gccgacgctt tacgcgcgaa
    17941 gagcttctgg ccgtcggtct ggatgccgcc atcattgacg atcctcatta tgtcaatatc
    18001 ggtacggtgt tagacaacgc cgactgcttc gacgccaccc tgtttggcta ttcgcgacag
    18061 gaagcggagt cgatggaccc gcagcagcgc ctatttttgc aggcggtctg gcatgcgctg
    18121 gaacatgccg gttatgcccc cggcgccgtc ccccataaga ccggcgtttt cgcctcttcc
    18181 cggatgagta cctaccccgg tcgcgaagca ttgaacgtga cagaagtcgc gcaggtaaaa
    18241 ggtctgcaat ctctgatggg caatgataaa gactatattg ccacccgcgc cgcgtacaaa
    18301 ctcaacctgc acggcccggc gttatcggta cagaccgcct gctccagctc gctggttgcc
    18361 gtgcatctgg cctgtgaaag cctgcgcgca ggcgaatccg atatggcggt tgccggcggc
    18421 gtggcgctct ctttccccca gcaggcaggc taccgctacc agcccggaat gattttctct
    18481 cctgatggtc actgtcgtcc ctttgacgcc tcggctgagg gcacctgggc cggtaacggt
    18541 ctcggctgcg tggtgctgcg tcgcctgaga gacgcgctgc tgtcaggcga tccgattatc
    18601 tcggtgatcc tctccagcgc ggtcaacaac gacggcaaca gaaaggtcgg ctataccgcc
    18661 ccttccgtcg cagggcaaca ggcagtcatc gaagaggcgt taatgctggc ggccatcgac
    18721 gacaggcagg taggttacat tgaaacccac ggcaccggca caccgctggg cgacgcgatt
    18781 gaaattgaag cgttacgcaa cgtctatgcg cctcgcccgc aggatcagcg ctgtgcgctc
    18841 ggttccgtga aaagtaacat gggccatctg gataccgcgg cgggcattgc cggactgctg
    18901 aaaaccgttc tggcagtcag tcgcgggcaa attcctccct tactgaattt tcacaccccc
    18961 aacccggcgc tgaaacttga agagagcccc tttaccatac cggtgtcggc acaggcatgg
    19021 caggacgaaa tgcgttatgc gggcgtctcc tcctttggta ttggcggcac caactgccat
    19081 atgatcgtcg cctcgctgcc cgacgcgctc aacgcgcgcc tccccaatac ggatagcggc
    19141 agaaaaagta ccgcgctgct gctcagcgcc gccagcgaca gcgcgttgcg gcggctggcg
    19201 acggattatg ccggggcgct gagagagaat gcggatgcca gctctctggc cttcacagcc
    19261 ctgcacgcgc gccgtctcga tctccccttc cgcctggcgg cgccattaaa ccgtgaaacc
    19321 gccgaggcgc tcagcgcctg ggccggtgag aaatcggggg cgctggttta cagcggccac
    19381 ggcgccagcg gcaagcaggt gtggctgttt accggccagg gctcgcactg gcgcactatg
    19441 ggtcaaacga tgtaccagca ctcaacggcg tttgccgaca cgctggatcg ctgtttttcc
    19501 gcctgtagcg aaatgctcac gccgtcactg cgcgaagcga tgtttaaccc cgattcggcg
    19561 caactggaca atatggcctg ggcgcagccg gcgattgtcg cgtttgaaat cgcgatggcg
    19621 gcgcactggc gtgctgaagg actgaagcca gacttcgcca ttgggcattc cgtcggtgaa
    19681 tttgccgctg ctgttgtctg cggacactat acgattgaac aggtcatgcc actggtttgt
    19741 cggcgcggcg cgctaatgca gcagtgcgca agcggcgcga tggtggcggt atttgcagac
    19801 gaagacacgc tgatgccgct ggctcgccag tttgagctgg atctcgccgc caataacggt
    19861 acgcaacata cggtattttc cgggccggaa gcccgtctcg cagtattttg cgccacgctc
    19921 tcgcagcatg acattaacta tcgtcgcctg agcgtaaccg gtgcggcgca ctccgcttta
    19981 ctggagccga tactcgatcg gttccaggac gcctgcgcgg gactgcacgc ggagccgggg
    20041 caaataccga ttatttccac gctcaccgcc gacgtcattg atgagtcaac gctcaaccag
    20101 gcggattact ggcgccgaca catgcgccag ccggtgcgtt ttatccagag tattcaggtg
    20161 gcgcatcagc tcggcgcccg cgtttttctg gagatggggc ccgatgccca gttggttgct
    20221 tgcgggcagc gcgaataccg cgataacgca tactggatag ccagcgcccg gcgtaacaaa
    20281 gaggcgagcg atgtcctcaa tcaggccctg ctccagcttt acgctgccgg cgtcgcccta
    20341 ccgtgggccg acctgctggc gggcgatgga caacgtatcg ctgcgccatg ttatccgttt
    20401 gatactgagc gttactggaa agagcgcgtc tccccggcct gcgagcctgc cgacgcagcg
    20461 ctgtctgccg ggctggaggt ggcgagtcgc gccgcgacag cgctcgatct ccctcgcctg
    20521 gaagcgctta aacagtgcgc cacgcgactg cacgccatct acgtcgatca actggtacaa
    20581 cgctgtaccg gcgatgccat tgagaacggc gtggacgcca tgaccatcat gcgccgtgga
    20641 cgtctgctgc cccgctacca gcagctactc cagcgcctgc tgaataactg cgtggtcgac
    20701 ggcgattacc gctgcaccga cgggcgatac gtccgcgccc gccccattga acatcaacag
    20761 cgggaatcac tgctgacgga acttgccggt tattgtgaag gttttcaggc tattcccgac
    20821 accatcgccc gtgccggcga tcggttatat gaaatgatga gcggcgcgga agaaccggtg
    20881 gcgattatct tcccgcaaag cgcctccgac ggcgtggaag tgctgtatca ggaattcagc
    20941 tttggccgct atttcaacca aatcgccgcc ggggtattac gcggcattgt ccagacgcgt
    21001 cagccccgcc agccgttgcg tattcttgaa gttggcggcg gaaccggcgg caccaccgcg
    21061 tggctgctgc cggaactcaa cggcgttccg gcactggagt accatttcac cgatatctcg
    21121 gcgctgttca cccgtcgcgc ccagcagaaa ttcgccgact atgattttgt gaagtatagc
    21181 gagctggatc tcgaaaaaga ggcgcagtct cagggtttcc aggcacagtc ttacgatctt
    21241 atcgtggcag cgaacgtgat tcacgccacc cgccatattg gccgcacgct cgataatctg
    21301 cgccccctgc tcaagccggg cgggcgcctg ctgatgcgcg aaatcaccca gccaatgcgt
    21361 ctgtttgact tcgttttcgg cccgctggtt cttccgctac aggatctcga cgcccgcgaa
    21421 ggtgagttat tcctcaccac cgctcagtgg caacaacagt gccgccacgc cggattcagc
    21481 aaagtggcgt ggctaccgca ggatggcagc ccgaccgccg ggatgagcga acatatcatt
    21541 ctcgccacgc tgcccggtca ggcggttagc gccgtaacat tcaccgcgcc atcagaaccc
    21601 gtgttggggc aggcgctgac ggataacggt gattatctcg ccgactggtc tgattgcgca
    21661 ggtcagcccg aacggtttaa cgcccgctgg caggaggcct ggcgtctgct ttcacagcgt
    21721 catggcgacg ctctgcctgt ggaaccgccc cccgtcgccg ccccggagtg gctggggaag
    21781 gttcgcttaa gctggcaaaa cgaagccttt tcccgcggtc agatgcgcgt tgaagcccgt
    21841 catcctgctg gcgagtggct gccgctatcg cccgccgcgc ctcttcctgc gccgcagacg
    21901 cattatcaat ggcgctggac gcccctcaac gtcgccagca ttgaccaacc gcttaccttt
    21961 agcttcagcg ccggtacgct tgcgcgcagc gacgagctgg cgcaatacgg catcattcac
    22021 gatccgcacg cctcttcgcg actgatgatt gttgaggaga gcgaggatac gctggcctta
    22081 gcggagaaag tgatagcagc gctcaccgcc agcgcagccg gattgattgt ggttactcgc
    22141 cgcgcgtggc gagtcgagga aaatgaagca ctctctgcat cccatcacgc gctatgggcc
    22201 ttgcttcgcg tcgcggccaa cgaacagccg gaacggttgc ttgccgccat cgatctcgcc
    22261 gaaaaccccc cgtgggaaac gctgcatcaa gggttgagcg cagtctcact atcacagcgc
    22321 tggctcgccg cacggggtga caccctttgg ctcccttcac tggcgcccaa tacgggatgc
    22381 gccgctgaat taccggcaaa cgtgtttacc ggcgatagcc gctggcatct ggtgaccgga
    22441 gcgtttggcg gattaggccg ccttgccgtg aactggctca gagaaaaagg ggcgcgacgc
    22501 atcgccctgc tggcgccgcg cgtggatgag tcatggctac gcgacgtgga gggcgggcag
    22561 acgcgcgtct gccgttgtga tgtgggcgat gccgggcaac tggccacggt tcttgacgat
    22621 ctggcggcca acggcggcat tgccggagcg attcatgccg ctggcgtatt ggctgacgcg
    22681 cccttgcagg agcttgatga ccaccagctg gctgccgttt tcgcggtaaa agcgcaggcg
    22741 gcaagccagc tgttgcaaac cctgcgcaac cacgacggac gctatcttat tctctactct
    22801 tccgctgccg ccaccctcgg cgcgccgggt cagagcgccc atgcgctggc ctgcggctac
    22861 ctggacgggc tggcccagca gttttccacc cttgatgcgc cgaaaacgct ctctgtcgcc
    22921 tggggcgcat ggggagaaag cggtcgggcg gccacgccgg aaatgctggc gacgctcgcc
    22981 agccgaggta tgggcgcgtt aagcgatgcc gaaggctgct ggcacctgga acaggcggtg
    23041 atgcgcggcg ccccgtggcg actggcgatg cgcgttttta ccgacaaaat gcccccgtta
    23101 caacaggctc tgtttaacat cagcgccaca gaaaaagccg caacgccggt cattcctcct
    23161 gctgatgaca acgcctttaa cggcagcctg agcgatgaaa cagcggtgat ggcatggctg
    23221 aaaaagcgga ttgcggttca gctaaggctg agcgatccgg cgtcactgca tccaaaccag
    23281 gatctgttgc aactcggcat ggactcgctg ctcttccttg aactcagtag cgatattcag
    23341 cactacctgg gcgtacgcat caatgcggaa cgggcgtggc aggatctgtc tcctcatgga
    23401 ctcacgcagc ttatctgttc taagccagag gcgacgcctg ccgcttcgca gccggaagtg
    23461 ttgcggcacg acgccgacga gcgttatgcg cccttccctt tgacgcccat tcagcacgcc
    23521 tactggctgg ggcgaaccca cctcattggc tatggcggcg tcgcctgtca cgtcctgttt
    23581 gagtgggata aacgccacga tgagttcaat ctcgccatac tggagaaagc atggaaccag
    23641 ctcatcgcac gccacgatat gttgcgtatg gtggttgatg ccgacgggca gcagcgaatc
    23701 ctggcgacaa cgccggagta tcacatcccg cgtgacgatc tgcgcgcgct ttccccggaa
    23761 gaacagcgca tcgcgctgga aaaacggcgg catgaactga gctatcgcgt tttgcctgcc
    23821 gaccagtggc ctctttttga gctggtggtc agcgaaatcg acgattgcca ttaccgtctg
    23881 catatgaacc tcgacctttt gcagtttgat gtgcagagtt ttaaagtcat gatggacgac
    23941 ctggcgcagg tctggcgcgg tgaaacgctg gcgccgctcg ctattacctt ccgtgattat
    24001 gtgatggctg aacaggcgcg ccgacagaca tcggcatggc acgatgcctg ggattactgg
    24061 caggaaaaac tgccgcaact gcccttagcg ccagagctgc cggtggttga gacgcccccg
    24121 gaaacgccac acttcaccac cttcaaatcg acgatcggca agacagaatg gcaggccgtg
    24181 aaacagcgct ggcagcagca aggcgtcaca ccgtctgccg cgctgctcac gctgtttgcc
    24241 gccacccttg agcgctggag ccgtaccaca acatttacgc tgaacctgac gttcttcaat
    24301 cgccagccga tccatccgca aatcaaccag ttgattggtg attttacctc cgtcacgctg
    24361 gttgatttta acttctcagc gccggtgacg ttgcaagagc agatgcaaca gacccaacag
    24421 cgcctctggc aaaacatggc gcacagtgaa atgaacggtg ttgaggtgat ccgtgagctg
    24481 ggccgcctgc gcggatcaca acgtcaaccg ctgatgccgg tagtgtttac cagtatgctg
    24541 gggatgacgc tggaaggcat gactatcgat caggcgatga gccatctgtt cggcgaaccc
    24601 tgctatgtat tcacgcaaac gccgcaggtc tggctggatc atcaggtcat ggagagcgac
    24661 ggcgagttga tgtttagctg gtactgcatg gacaacgtgc tggaacccgg cgctgccgag
    24721 gcgatgttta atgactattg cgccatcctg caagccgtca tcgccgcccc tgaaagcctg
    24781 aagactctcg ccagcggcat cgccgggcac attccccgcc gacgctggcc gctgaacgcg
    24841 caggcggact acgatctgcg ggatattgag caggcgacgc tcgaataccc cggcatccgg
    24901 caggccagag cggaaataac cgaacagggc gcgttgacgc tggatatcgt gatggccgac
    24961 gatccgtcgc catcagcggc gatgcctgat gagcacgaac ttacccaact ggcgctgccg
    25021 ttgcctgagc aggcgcagct tgatgagctg gaggcgacct ggcgctggct ggaggcgcgt
    25081 gcgctacagg ggatcgcggc tacgctaaat cgtcacggcc tgtttaccac gccggagatc
    25141 gcccatcgct ttagcgcaat agtacaggcg ctgtccgcgc aagcgtctca ccagcgtctg
    25201 ctgcgccagt ggctacagtg tctgacggaa agagagtggt taatccgcga aggtgaaagc
    25261 tggcgctgcc gcattccgct cagcgagatt cctgagcctc aggaagcgtg cccgcaaagc
    25321 caatggagcc aggcgctggc gcagtatctg gaaacctgca tcgcccggca cgacgccctc
    25381 ttctccgggc ggtgttctcc gctggaattg ctgttcaacg agcagcatcg cgttaccgac
    25441 gcgctgtatc gcgacaaccc cgccagcgcc tgtctgaatc gctataccgc gcagattgcc
    25501 gccttgtgca gcgcagaacg gattctggag gttggcgccg gaaccgcagc cactaccgcg
    25561 ccggtgctga aggccacgcg gaacacgcgg cagtcgtacc acttcacgga cgtctccgcg
    25621 cagttcctca atgacgccag agcccgtttc catgatgaat cgcaggtgtc ttatgccttg
    25681 ttcgacatca accagccgct ggatttcacc gcccacccgg aggcgggtta cgacctgatc
    25741 gttgccgtca atgtgctcca cgacgccagc catgtcgtcc agacgttgcg cagattaaaa
    25801 ctgttgctga aagccggcgg acgtttgctg atcgttgaag cgacggagcg aaacagcgta
    25861 ttccagctgg cgagcgtggg ctttattgag ggattaagcg gataccgcga tttccgccgc
    25921 cgggatgaga aaccgatgct cacccgctcc gcatggcagg aggttctcgt tcaggccggg
    25981 tttgcaaacg agctggcgtg gcccgcgcag gaatcgtcgc cgctgcgcca gcatctgctg
    26041 gtggcgcgtt cgcctggcgt aaatcgcccg gataaaaaag ccgtgagccg ctatttacag
    26101 cagcgctttg gcaccggtct gcccatttta cagatccggc aaagagaagc gttatttacg
    26161 ccgctgcatg ccccgtctga tacgccgact gagccagcca aacccacgcc agttgccggg
    26221 gggaatccgg cgctggaaaa acaggtggct gaactctggc aatcgctgct gtctcgcccc
    26281 gtggcaaggc atcacgactt tttcgaactg ggcggcgaca gcctgatggc gacaaggatg
    26341 gtcgcgcagc taaaccggag agggattgcc agggctaacc ttcaggatct gttcagccat
    26401 tcgacgctga gcgacttctg cgcccatcta caggcggcta cgtcaggaga ggacaacccg
    26461 ataccccttt gccagggcga cggtgaggaa accctgtttg tcttccacgc ttcagacggc
    26521 gatatcagcg cctggctgcc gctcgctagc gcgttgaaca ggcgcgtttt cggcctgcaa
    26581 gcaaaatcgc cgcagcgctt tgccacgctc gaccagatga tcgatgagta tgtcgggtgc
    26641 atccgtcgtc agcagcctca cggcccttat gtgctggcgg gttggtcgta tggcgcgttt
    26701 cttgcggcgg gcgccgcaca gcgcctgtac gccaaaggcg agcaggttcg gatggtgtta
    26761 atcgatcccg tgtgccgaca ggatttctgt tgcgaaaacc gggcggccct gctgcgcctg
    26821 ttagccgaag gacaaacgcc tctggcactg cccgaacatt tcgaccagca gacgcccgac
    26881 agccagcttg ccgactttat cagcctcgct aaaacggccg gtatggtgtc gcaaaacctg
    26941 acgctgcaag cggcagaaac gtggctcgac aacatcgcgc atctgctgcg tttactgact
    27001 gagcatacgc cgggcgaaag cgttccggtc ccctgtctca tggtgtatgc agccgggaga
    27061 cccgcgcgct ggacgccagc agaaaccgag tggcagggct ggataaacaa cgccgacgac
    27121 gctgtgattg aagccagcca ctggcaaatc atgatggaag ccccccacgt tcaggcttgt
    27181 gcgcaacaca ttacgcgctg gctttgcgca acctcaatgc aaccggagaa cacgttatga
    27241 tgccgtccgc ctccccaaaa caacgcgtac tgattgtggg cgccaaattt ggcgaaatgt
    27301 acctgaatgc ctttatgcag cccccggagg ggctggaact ggtcggcctg ctggcgcagg
    27361 gaagcgcccg ttcaagagag ctggctcatg cgtttggcat tccgctgtat acctcgccgg
    27421 aacagataac caagatgccg gatatcgcct gtattgtcgt gcgttcgacc gtcgccggag
    27481 gaaccggtac gcagcttgcc agacactttc tgacgcgcgg cgtacacgtc attcaggagc
    27541 atcccctcca cccggatgac atcagttcct tacaaacgct ggcgcaggaa cagggctgct
    27601 gctattgggt aaacacgttt tatccgcata cgcgcgcggg acgcacatgg cttcgcgatg
    27661 ctcagcagct gcgtcgctgc ctggctaaga cgccgccggt agtccacgcc accaccagcc
    27721 gacagttgct ctactccact ctggatttgt tgttgctggc tctgggcgtt gatgccgccg
    27781 ccgtggagtg cgacgtggtt ggcagcttta gcgattttca ctgtctgaga ctcttctggc
    27841 cggagggaga agcctgcctg ctgcttcagc gctatctcga tcctgacgat ccagatatgc
    27901 atagccttat catgcaccgc ctgctgctgg gctggccgga aggtcatctt tcgctggagg
    27961 cgagctacgg ccccgtcatc tggtcatcca gcctgtttgt cgcagaccat caggagaacg
    28021 cccacagcct ctaccgcaga ccggagatcc tgcgcgatct gccgggtctg acgcgcagcg
    28081 cggcgcccct gagctggcgc gactgctgcg aaaccgtcgg gccggagggc gtcagctggt
    28141 tgctacacca attgcgcagc catctggcgg gcgaacatcc accagcggcc tgccaaagcg
    28201 tacatcagat cgcgttatca cgtttatggc agcagatttt acgtaagacc ggcaatgcgg
    28261 agatcagacg cctgacaccg ccgcaccacg accggttagc cggtttctac aacgatgatg
    28321 ataaggaggc gctgtgacgc aatctgcaat atgcatcccg ctgtggcccg cccggaacgg
    28381 caatactgcg catctggtca tgtgcccttt cgctggcggc agcagtagcg cgtttcgcca
    28441 ctggcaagct gagcaactgg ctgattgcgc gctttctctg gtgacctggc cggggcgcga
    28501 tcgccttcgc catctggaac cgctcagaag cattacacaa ctggcggcac tgctggcgaa
    28561 cgagctggaa gcatccgtat cgcctgacac gccgctttta ctcgccgggc acagcatggg
    28621 ggcgcaggtg gcgtttgaaa cctgccgact tctggagcaa cgggggcttg cgccacaagg
    28681 actgattatt tccgggtgcc atgccccgca tctgcattct gaacgccagc tcagccatcg
    28741 cgatgatgcc gactttatcg ctgagctgat agacattggc ggatgttctc ctgaactgcg
    28801 ggaaaaccag gaattaatgt cgctgtttct tcctcttctg cgcgctgatt tttacgccac
    28861 cgagagctat cactacgact cgcccgacgt ctgtccgccg ctgcgcacgc ctgcgctgtt
    28921 attgtgcggc agccacgatc gcgaagcctc ctggcagcag gtcgatgcct ggcgtcagtg
    28981 gctgagccac gttacaggcc cggtggtgat tgacggcgat catttctatc ccattcaaca
    29041 agcccggtcc ttttttacgc agattgtccg ccattttccc cacgcatttt ctgcaatgac
    29101 cgcgttgcaa aaacagccca gtacttcaga aaggtgacgc atgaattctt cctttaaatc
    29161 tctgattgaa cagtatccct tacccattgc cgaacagttg cgccactggg cggcccgtta
    29221 tgcctcgcga attgccgtcg ttgatgcaaa ggggtcgtta acctacagcg cgcttgatgc
    29281 acaagttgac gaacttgccg caggtctgtc atcactgggt ttgcgttcgg gggagcatgt
    29341 aattgtgcag cttcccaacg acaacgcgtt tgttaccctg ctgttcgcct tgttaagact
    29401 gggcgttatc cccgtgctgg cgatgccctc gcaacgggcg ctggatatcg acgcgctgat
    29461 tgagctggcg caacccgtcg cttacgttat tcacggggaa aaccacgcag agctggcccg
    29521 acagatggcg cacaaacacg cctgcttgcg tcatgttctg gtcgctggag agaccgtgag
    29581 cgacgatttt acgccgttct tctcccttca cggtgagcga caggcatggc cgcagcctga
    29641 tgtttccgcc accgcgttgt tgttgctctc aggcggcaca accggcacgc ccaaactcat
    29701 cccgcgccga catgccgact atagctataa cttcagcgct tctgctgaac tgtgcggcat
    29761 cagccaacag agcgtgtatc tcgccgtcct cccggtggcg cataactttc cgctggcctg
    29821 ccccggtatt ctgggaacgc ttgcctgcgg cggaaaagtg gtgctgaccg acagcgccag
    29881 ctgtgatgag gtgatgcctt taatcgcgca ggaaagagtg actcacgtcg ccctggttcc
    29941 ggcgctggcg caattatggg tgcaggccag ggagtgggaa gacagcgacc tttcgtcgct
    30001 gcgcgtcatt caggcaggcg gcgcccggct cgacccgacg cttgctgagc aggttatcgc
    30061 cacctttgac tgtaccctgc aacaggtttt cggtatggcg gaaggcctgc tctgttttac
    30121 ccggctggac gatccgcatg ccaccattct ccacagccag gggcgcccgt tgtcccctct
    30181 ggatgaaatc cgcatcgttg atcaagacga gaacgacgtc gcgccgggcg aaaccgggca
    30241 attgttaacg cgcggccctt ataccatttc gggctattac cgcgcccctg cccacaacgc
    30301 gcaggccttt accgcgcaag ggttttaccg cacaggcgac aatgtcaggc tggatgaggt
    30361 ggggaacctg cacgttgagg gacgcataaa agagcagatc aaccgcgccg gagaaaaaat
    30421 agccgcggct gaagtggaat cggcactgct gcgtttagcg gaagtgcaag attgcgcggt
    30481 ggtcgccgcg ccggacacgc tgcttggcga gcggatttgc gcgtttatca tcgcgcagca
    30541 ggtgccaact gactatcagc agttgcgtca acaactgacc cgtatggggc tcagcgcgtg
    30601 gaaaattcct gaccaaatcg agtttctgga ccactggccg ctcaccgccg tcggcaagat
    30661 agacaaaaaa cgcctgacgg ctctcgccgt cgaccgttat cgccattctg cccaataagc
    30721 gcaaaccgac ccgaaacagg ttgaaataaa cccgtttcgg gtagcaccac tattagaaat
    30781 agttatcatt ttcaattcac cattgtcggt atttttggcg tttcgccgtc ttacagggac
    30841 tcacaacaat gaaaatgaca cggctttatc ctctggcctt ggggggatta ttgctccccg
    30901 ccattgctaa tgcccagact tcacagcaag acgaaagcac gctggtggtt accgccagta
    30961 aacaatcttc ccgctcggca tcagccaaca acgtctcgtc tactgttgtc agcgcgccgg
    31021 aattaagcga cgccggcgtc accgccagcg acaaactccc cagagtcttg cccgggctca
    31081 atattgaaaa tagcggcaac atgctttttt cgacgatctc gctacgcggc gtctcttcag
    31141 cgcaggactt ctataacccc gccgtcaccc tgtatgtcga tggcgtccct cagctttcca
    31201 ccaacaccat ccaggcgctt accgatgtgc aaagcgtgga gttgctgcga ggcccacagg
    31261 gaacgttata tggcaaaagc gctcagggcg ggatcatcaa catcgtcacc cagcagccgg
    31321 acagcacgcc gcgcggctat attgaaggcg gcgtcagtag ccgcgacagt tatcgaagta
    31381 agttcaacct gagcggcccc attcaggatg gcctgctgta cggcagcgtc accctgttac
    31441 gccaggttga tgacggcgac atgattaacc ccgcgacggg aagcgatgac ttaggcggca
    31501 cccgcgccag catagggaat gtgaaactgc gtctggcgcc ggacgatcag ccctgggaaa
    31561 tgggctttgc cgcctcacgc gaatgtaccc gcgccaccca ggacgcctat gtgggatgga
    31621 atgatattaa gggccgtaag ctgtcgatca gcgatggttc accagacccg tacatgcggc
    31681 gctgcactga cagccagacc ctgagtggga aatacaccac cgatgactgg gttttcaacc
    31741 tgatcagcgc ctggcagcag cagcattatt cgcgcacctt cccttccggt tcgttaatcg
    31801 tcaatatgcc tcagcgctgg aatcaggatg tgcaggagct gcgcgccgca accctgggcg
    31861 atgcgcgtac cgttgatatg gtgtttgggc tgtaccggca gaacacccgc gagaagttaa
    31921 attcagccta cgacatgccg acaatgcctt atttaagcag taccggctat accaccgctg
    31981 aaacgctggc cgcatacagt gacctgacct ggcatttaac cgatcgtttt gatatcggcg
    32041 gcggcgtgcg cttctcgcat gataaatcca gtacacaata tcacggcagc atgctcggca
    32101 acccgtttgg cgaccagggt aagagcaatg acgatcaagt gctcgggcag ctatccgcag
    32161 gctatatgct gaccgatgac tggagagtgt atacccgtgt agcccaggga tataaacctt
    32221 ccgggtacaa catcgtgcct actgcgggtc ttgatgccaa accgttcgtc gccgagaaat
    32281 ccatcaacta tgaacttggc acccgctacg aaaccgctga cgtcacgctg caagccgcga
    32341 cgttttatac ccacaccaaa gacatgcagc tttactctgg cccggtcggg atgcagacat
    32401 taagcaatgc gggtaaagcc gacgccaccg gcgttgagct tgaagcgaag tggcggtttg
    32461 cgccaggctg gtcatgggat atcaatggca acgtgatccg ttccgaattc accaatgaca
    32521 gtgagttgta tcacggtaac cgggtgccgt tcgtaccacg ttatggcgcg ggaagcagcg
    32581 tgaacggcgt gattgatacg cgctatggcg cactgatgcc ccgactggcg gttaatctgg
    32641 tcgggccgca ttatttcgat ggcgacaacc agttgcggca aggcacctat gccaccctgg
    32701 acagcagcct gggctggcag gcgactgaac ggatgaacat ttccgtctat gtcgataacc
    32761 tgttcgaccg tcgttaccgt acctatggct acatgaacgg cagcagcgcc gtcgcgcagg
    32821 tcaatatggg tcgcaccgtc ggtatcaata cgcgaattga tttcttctga ttattgtaaa
    32881 agggataccg aaaaggtatc ccttttacac cactagttaa aaccagtaac tcagcagagt
    32941 cgcaaaaaat attaatccat agtg
//
LOCUS       DAJOWB010000043        32395 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJOWB010000043 DAJOWB010000000
VERSION     DAJOWB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.043"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            40..1329
                     /locus_tag="OHO85_002192"
     CDS             40..1329
                     /locus_tag="OHO85_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCQ2671560.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVGEEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARSVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELLQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            1326..2792
                     /gene="hpaE"
                     /locus_tag="OHO85_002193"
     CDS             1326..2792
                     /gene="hpaE"
                     /locus_tag="OHO85_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCQ2671561.1"
                     /translation="MKKVNHWINGKNFAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPRWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2794..3645
                     /gene="hpaD"
                     /locus_tag="OHO85_002194"
     CDS             2794..3645
                     /gene="hpaD"
                     /locus_tag="OHO85_002194"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCQ2671562.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3655..4035
                     /locus_tag="OHO85_002195"
     CDS             3655..4035
                     /locus_tag="OHO85_002195"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCQ2671563.1"
                     /translation="MPHFIVECTDNIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            4103..4906
                     /gene="hpaH"
                     /locus_tag="OHO85_002196"
     CDS             4103..4906
                     /gene="hpaH"
                     /locus_tag="OHO85_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCQ2671564.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4917..5705
                     /gene="hpaI"
                     /locus_tag="OHO85_002197"
     CDS             4917..5705
                     /gene="hpaI"
                     /locus_tag="OHO85_002197"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCQ2671565.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLLQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            5880..7256
                     /gene="hpaX"
                     /locus_tag="OHO85_002198"
     CDS             5880..7256
                     /gene="hpaX"
                     /locus_tag="OHO85_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCQ2671566.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7266..8156
                     /gene="hpaA"
                     /locus_tag="OHO85_002199"
     CDS             7266..8156
                     /gene="hpaA"
                     /locus_tag="OHO85_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCQ2671567.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8407..9969
                     /gene="hpaB"
                     /locus_tag="OHO85_002200"
     CDS             8407..9969
                     /gene="hpaB"
                     /locus_tag="OHO85_002200"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCQ2671568.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            9987..10499
                     /locus_tag="OHO85_002201"
     CDS             9987..10499
                     /locus_tag="OHO85_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCQ2671569.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10892..13042
                     /gene="btsT"
                     /locus_tag="OHO85_002202"
     CDS             10892..13042
                     /gene="btsT"
                     /locus_tag="OHO85_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCQ2671570.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLKVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13092..13295
                     /locus_tag="OHO85_002203"
     CDS             13092..13295
                     /locus_tag="OHO85_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCQ2671571.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13306..14262
                     /gene="yjiA"
                     /locus_tag="OHO85_002204"
     CDS             13306..14262
                     /gene="yjiA"
                     /locus_tag="OHO85_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCQ2671572.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRSAFAGLKK"
     gene            complement(14340..16879)
                     /locus_tag="OHO85_002205"
                     /pseudo
     CDS             complement(14340..16879)
                     /locus_tag="OHO85_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
     gene            complement(17231..17395)
                     /locus_tag="OHO85_002206"
     CDS             complement(17231..17395)
                     /locus_tag="OHO85_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HCQ2671573.1"
                     /translation="MEFHENKAKTPFIGFVQLWQAVRRWGRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            17572..18984
                     /locus_tag="OHO85_002207"
     CDS             17572..18984
                     /locus_tag="OHO85_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCQ2671574.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITLQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQIYQRNLALYTCWIREYFPCEIY
                     ITRPKGGFLLWIELPEQVDMVCVARQLYRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETNREALKQIGDAVYRAME"
     gene            complement(19138..20058)
                     /locus_tag="OHO85_002208"
     CDS             complement(19138..20058)
                     /locus_tag="OHO85_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCQ2671575.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRQLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMELIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKKFISELTRRMPHHRERIMTIAERIYNDGCIKGEQRILRLFLQNGVDPEW
                     IQKITGLSAEQMQALEQPLPDREHDSWLEY"
     gene            20525..21757
                     /gene="mdtM"
                     /locus_tag="OHO85_002209"
     CDS             20525..21757
                     /gene="mdtM"
                     /locus_tag="OHO85_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HCQ2671576.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGATTISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGIIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            21798..23078
                     /locus_tag="OHO85_002210"
     CDS             21798..23078
                     /locus_tag="OHO85_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCQ2671577.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNERHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            23194..24345
                     /locus_tag="OHO85_002211"
     CDS             23194..24345
                     /locus_tag="OHO85_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HCQ2671578.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTIEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLALRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            24355..25122
                     /locus_tag="OHO85_002212"
     CDS             24355..25122
                     /locus_tag="OHO85_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2671579.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            25119..25376
                     /locus_tag="OHO85_002213"
     CDS             25119..25376
                     /locus_tag="OHO85_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HCQ2671580.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            25441..26301
                     /locus_tag="OHO85_002214"
     CDS             25441..26301
                     /locus_tag="OHO85_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HCQ2671581.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNIYIVSEPNR
                     FYRFTPQSSH"
     gene            26369..27547
                     /locus_tag="OHO85_002215"
     CDS             26369..27547
                     /locus_tag="OHO85_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2671582.1"
                     /translation="MPSSTHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFSWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(27560..28114)
                     /gene="kptA"
                     /locus_tag="OHO85_002216"
     CDS             complement(27560..28114)
                     /gene="kptA"
                     /locus_tag="OHO85_002216"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCQ2671583.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            28364..29047
                     /locus_tag="OHO85_002217"
     CDS             28364..29047
                     /locus_tag="OHO85_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2671584.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            29044..29505
                     /locus_tag="OHO85_002218"
     CDS             29044..29505
                     /locus_tag="OHO85_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCQ2671585.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            29518..30690
                     /gene="iadA"
                     /locus_tag="OHO85_002219"
     CDS             29518..30690
                     /gene="iadA"
                     /locus_tag="OHO85_002219"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCQ2671586.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            30755..31666
                     /gene="hypT"
                     /locus_tag="OHO85_002220"
                     /gene_synonym="qseD"
     CDS             30755..31666
                     /gene="hypT"
                     /locus_tag="OHO85_002220"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCQ2671587.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(31659..32051)
                     /gene="iraD"
                     /locus_tag="OHO85_002221"
     CDS             complement(31659..32051)
                     /gene="iraD"
                     /locus_tag="OHO85_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCQ2671588.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHILAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(32048..32131)
                     /gene="idlP"
                     /locus_tag="OHO85_002222"
     CDS             complement(32048..32131)
                     /gene="idlP"
                     /locus_tag="OHO85_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCQ2671589.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         7475 a         8550 c         8455 g         7915 t
ORIGIN      
        1 catattaatg attaaaatga tcgaatccga ggagtggtaa tgaaaggcac tatcttcgcc
       61 gtagcgttga accatcgcag ccaacttgat gcatggcagg aagcgttcca gcaatccccc
      121 tacaaagccc cgcctaaaac tgcggtctgg tttattaaac cgcgcaatac ggtgattggt
      181 tgcggtgaac cgattccctt tccacagggt gaaaaggtac tgagcggtgc gactgttgcg
      241 ctgattgtgg gaaaaacggc gacgaaagta ggtgaagaag atgcggcaga gtacatcgcc
      301 ggatatgcgc tggctaacga cgtcagcctg ccggaagaga gcttttaccg cccggcaatc
      361 aaagcaaaat gtcgtgatgg attctgcccc attggcgaaa ccgtggctct cagcaatgtc
      421 gataatctga ccatctatac cgagatcaac gggcgtcctg ccgatcactg gaataccgcc
      481 gatttacaac gtaacgccgc gcagttgctg agcgccctga gcgaatttgc cacactgaat
      541 ccaggcgatg ccattctgct cggcacgcca caggcgcgcg tggaaataca gccaggcgat
      601 cgcgttcgtg ttctcgcaga aggtttcccg ccgctggaaa atccggtagt ggacgaacgt
      661 gaagtgacca cgcgcaagag cttcccaacg cagccacacc cgcacggcac gctgtttgcc
      721 ctcggcctga actacgccga ccacgccagc gaactggaat ttaagccacc ggaagaaccg
      781 ctggtgttcc tgaaagcgcc aaataccctc actggcgata accagacctc cgtgcgtcca
      841 aacaatattg aatacatgca ctatgaagcg gagctggtgg tagttattgg caaacaggcg
      901 cgtagcgtca gcgaagccga tgccatggat tatgtcgcgg gctacaccgt gtgtaacgac
      961 tacgccattc gcgactatct ggaaaactac taccgcccta acctgcgggt aaaaagccgc
     1021 gacggactga cgccgatgct ttcaaccatc gtgccgaaag aggcgatccc ggacccgcat
     1081 aatctgaccc ttcgcacctt cgtcaacggc gagttactcc agcaaggcac caccgccgat
     1141 ctgatcttca gcgtgccctt cctgatcgcc tacttaagcg aatttatgac cctgaatccg
     1201 ggcgacatga tcgccaccgg cacaccaaaa ggcttatctg acgtggtgcc tggcgatgaa
     1261 gtagtggtgg aagtagaagg cgtgggccgc ctggtgaacc gaattgtgag tgaggaaaca
     1321 gcgaaatgaa aaaagtaaat cattggatca acggcaaaaa ttttgcaggt aacgactact
     1381 tccagaccac caatccggca acgggtgaag tgctggcgga tgtggcctct ggcggtgaag
     1441 cggagatcaa tcaggcggta gcggcagcga aagaggcgtt cccgagatgg gccaatctgc
     1501 caatgaaaga gcgtgcgcgc ctgatgcgcc gtctgggcga tctgatcgac cagaacgtgc
     1561 cagagatcgc cgcgatggaa accgcggaca ccggcctgcc gatccatcag accaaaaatg
     1621 tgttgatccc acgcgcttcc cacaactttg aatttttcgc ggaagtctgc cagcagatga
     1681 acggcaagac ctatccggtt gacgacaaga tgctcaacta cacgctggtg cagccggtgg
     1741 gcgtttgtgc gctggtatcg ccgtggaacg taccgtttat gaccgccaca tggaaggtcg
     1801 cgccgtgtct ggcgctgggc aataccgcgg tactgaaaat gtcggaactc tccccgctga
     1861 ccgctgaccg cctgggtgag ctggcgctgg aagccggtat tccggcaggc gtgctgaacg
     1921 tggtacaggg ctacggcgca accgcagggg atgcgctggt tcgtcatcat gacgtacgtg
     1981 ccgtgtcgtt caccggcggt acggccaccg ggcgcaacat catgaaaaac gccgggctga
     2041 aaaaatactc catggaactg ggcggtaaat cgccggtgct gatttttgaa gatgccgata
     2101 ttgaacgcgc gctggacgcc gccctgttca ccatcttctc gatcaacggc gaacgctgca
     2161 ccgccggttc gcgcatcttt attcagcaaa gcatctaccc ggaattcgtt aaacgctttg
     2221 ccgaacgcgc caaccgtctg cgcgtgggcg atccgaacga tccgaatacc caggttggcg
     2281 cgcttattag ccagcagcac tgggaaaaag tctccggcta tatccgtctc ggcattgaag
     2341 aaggcgcaac cctgctggcg ggcggcccgg ataaaccgtc cgacctgcct gcgcacctga
     2401 aaggcggcaa cttcctgcgc ccaaccgtgc tggcagacgt tgataaccgt atgcgcgtcg
     2461 cccaggaaga gattttcggg ccggtcgcct gcctgctgcc gtttaaagac gaagccgaag
     2521 gcttacgtct ggcaaacgac gtggagtacg gcctggcatc gtacatctgg acgcaggatg
     2581 tcagcaaagt gttacgcctg gcgcgtggca ttgaagctgg catggtgttc gtcaacaccc
     2641 agaacgtgcg cgacctgcgc cagccatttg gcggcgtaaa agcctccggc accgggcgtg
     2701 aaggcggtga gtacagcttc gaagtgttcg cggaaatgaa gaacgtctgc atttccatgg
     2761 gcgaccatcc aattccgaaa tggggagtct gatatgggta agttagcgtt agccgccaag
     2821 atcacgcacg taccgtcgat gtatctctct gaactgccag ggaaaaacca cggttgccgc
     2881 cagggcgcga tcgacgggca taaagagatc agcaagcgtt gccgggaaat gggcgtcgat
     2941 accattatcg ttttcgatac ccactggctg gtcaacagtg cttatcacat caactgtgca
     3001 gaccattttg aaggcgtcta caccagcaac gagctgccgc attttatccg cgatatgacc
     3061 tacaactacg agggcaaccc ggagttgggg cagcttattg ccgatgaagc cttaaagctc
     3121 ggcgtgcggg caaaagcgca caacattccc agcctgaaac tggaatacgg cacgctggta
     3181 ccgatgcgct acatgaatga agacaagcac ttcaaagtgg tctccatttc ggctttctgc
     3241 acggttcacg attttgctga cagccgcaag ctgggcgaag cgattctgaa agcgatcgaa
     3301 cagtacgacg gcaccgtggc ggtccttgcc agcggttcgt tatcgcaccg ctttattgac
     3361 gatcagcgtg cagaagaagg gatgaacagc tacacccgcg agttcgaccg ccagatggac
     3421 gagcgcgtgg tgaagttgtg gcgcgaaggc cagttcaaag agttctgcaa tatgctgccg
     3481 gagtacgccg actactgcta cggcgaaggc aatatgcacg acacggtgat gctgctgggg
     3541 atgctcggct gggataaata cgacggcaag gtggagttta tcaccgagct attcccaagc
     3601 tctggcaccg gtcaggttaa cgctgttttc ccgcttcccg cgtaaggaac caccatgccg
     3661 cactttatcg tggaatgtac tgataatatt cgtgaagaag cccgcttacc tgagctgttt
     3721 gccagtgtta acactgcact ggcggcaacg gggatttttc cgcttggggg aatacgtagc
     3781 cgggcgcact ggatcgatac ctggcaaatg gctgatggca agcacgatta cgcctttgtg
     3841 catatgacgc taaaaatcgg cagcgggcgc agcctggaaa gccgtcagga agtgggcgaa
     3901 atgctgtttg atctgattaa aacgcacttc gcctcactga tggagagccg ttacctggcg
     3961 ctttcatttg agattgcaga gctgcacccg acgctgaatt ttaaacaaaa caacgtgcac
     4021 gcgttgttta aataaatttg caccagcttc caggccggat gataaggcgc agccgcatcc
     4081 ggcaatgcca caggatatcg ctatgttcga taaacacacc cacaccctga tcgcccagcg
     4141 tctggatcag gcagaaaaac agcgcgaaca gatccgcgcg atctcgctgg attacccgga
     4201 gatcaccatc gaagacgctt acgcggtgca gcgcgaatgg gttcgcctga aaatcgccga
     4261 aggtcgcacg ctgaaaggcc acaaaatcgg cctgacctcg aaagcgatgc aggcaagctc
     4321 gcagatcagc gaaccggatt acggcgcgct gctggatgac atgttcttcc acgatggcag
     4381 cgatatcccg accgatcgct ttatcgtgcc gcgcattgaa gtggagctgg ctttcgtgct
     4441 ggcaaaaccg ctgcgtgggc caaactgcac gctgttcgac gtttacaacg ccacggacta
     4501 tgtgatcccg gcgctggagc tgatcgacgc tcgctgccac aacatcgatc cggaaaccca
     4561 gcgcccgcgc aaagtgttcg acaccatttc tgataacgcc gccaacgccg gagtgatcct
     4621 cggcggtcgt ccgatcaaac cagacgaact ggatctgcgt tggatctccg ccctgatgta
     4681 tcgcaatggc gtgattgaag aaaccggcgt cgccgctggt gtgctgaatc atccggcaaa
     4741 cggcgtggcc tggctggcga acaaactcgc accctatgac gtacaactgg aagccgggca
     4801 aatcattctc ggtggttcgt tcacccgccc ggttccggcg cgtaagggcg acaccttcca
     4861 cgtcgattac ggcaacatgg gttccattag ctgccgcttt gtttaaggag agaacgatgg
     4921 aaaacagttt taaagcggcg ctgaaagcag gccgcccgca gatcggatta tggctggggc
     4981 tgagcagcag ctacagcgcg gagttactgg ccggagcagg attcgactgg ttattgatcg
     5041 acggtgagca cgcgccgaat aacgtgcaaa ccgtgctcac ccagctacag gcgattgcgc
     5101 cctaccccag ccagccggta gtacgtccgt cgtggaacga tccggtgcaa atcaaacaac
     5161 tgctggacgt cggcacacaa accttgctgg tgccgatggt acaaaacgct gacgaagccc
     5221 gtgaagcggt acgcgccacc cgttatcccc ccgccggtat tcgcggtgtg ggcagtgcgc
     5281 tggcccgagc ctcgcgctgg aatcgcattc ctgattacct gcaaaaagcc aacgatcaaa
     5341 tgtgcgtgct ggtgcagatc gaaacgcgtg aggcaatgaa gaacttactg cagattctgg
     5401 acgtggaagg ggtcgacggc gtgtttatcg gcccggcgga tctgagcgcc gatatgggtt
     5461 atgccggtaa tccgcagcac ccggaagtac aggccgccat tgagcaggcg atcgtgcaga
     5521 tccgtgaatc gggcaaagcg ccggggatcc tgatcgccaa tgagcaactg gcaaaacgct
     5581 atctggaact gggcgcgctg tttgtcgccg tcggcgttga caccaccctg ctcgcccgcg
     5641 ccgctgaagc gctggcagca cggtttggtg cgcaggccac cgccgtgaag cccggcgtgt
     5701 attaatgccg gatgcgctgc gcttatccgg cctacactcg caccaaaccg taggccggat
     5761 aaggcgttta cgccgcatcc ggcaaaaagc cgtaccaaat cgcggaccgg ataaggcgtt
     5821 tgtgcgcatc cgccataaac cttgtcgtac cctacaaaaa tcccattaga ggaagaaaaa
     5881 tgagcgacac ctcacctgcc ataccggaga gtatcgatcc ggcgaatcag cataaagcgc
     5941 tgactgccgg acaacaggcg gttattaaga agctatttcg ccgcctgatc gtctttctgt
     6001 tcgtgctgtt tatcttctcg ttccttgatc gcatcaacat cggctttgcc ggactcacga
     6061 tgggacgcga cctcggtctg agcgccacca tgtttggcct cgctaccacc ctgttctatg
     6121 ccgcttatgt catcttcggc attcccagca acattatgct gagtattgtc ggtgcgcggc
     6181 gctggatcgc caccatcatg gtgctctggg gcatcgcctc tactgccacc atgtttgcca
     6241 ctggccccac cagcttgtac gtactgcgta tactggttgg cattaccgaa gccggctttc
     6301 tgcctggtat tctgctgtat ttaaccttct ggtttccggc ctatttccgc gcccgtgcca
     6361 acgccttgtt tatggtagca atgccggtaa cgacagcgtt gggctcgatc gtttccggct
     6421 acattttgtc gctggatggc gtcatggcat taaaaggctg gcagtggctg tttttgctgg
     6481 aaggcttccc gtcggtatta ctcggcgtca tggtgtggtt ctggcttgat gactcaccgg
     6541 acaaagctaa gtggctgacg aaagaagaca aaaaatgcct gcaagagatg atggataacg
     6601 atcgtctgac gctggttcag ccagagggag ccatcagcca ccacgccatg caacaacgca
     6661 gtatgtggcg ggagatcttc actccggtgg tgatgatgta taccctggcg tatttctgcc
     6721 tgaccaacac acttagtgcg atcagcatct ggacaccgca gatcctgcaa agttttaatc
     6781 agggcagcag taatatcacc atcggcctgc tggccgccgt accgcagatt tgtaccattc
     6841 tcgggatggt ctactggagc cgtcactcag atcgccgcca ggaacgaagg catcacaccg
     6901 cccttcctta tttgttcgct gccgcaggtt ggttactggc ttcggcaact gatcacaaca
     6961 tgatccagat gctggggatc attatggctt cgaccggatc attcagcgca atggcgattt
     7021 tctggacaac accggatcag tccatcagcc tgcgggcacg agcgatcggt attgcggtga
     7081 tcaacgccac tggcaacatt ggttcagcgt taagtccgtt tatgatcggc tggttgaaag
     7141 atttgaccgg cagctttaac agtggattgt ggtttgttgc cgcgctgctg gtgattggtg
     7201 cggggattat ctgggcgatt ccaatgcagt cctcccgtcc gcgagcgacc ccgtaaggaa
     7261 cgacgatgtg tgaccgtcag attgccaata ttgatatcag caaagagtac gatgaaagcc
     7321 tgggcacgga cgatgtgcat tatcagtcgt tcgcccgcat ggcggcattt tttggccgcc
     7381 atatgctgcc acatcgccac gaacagtact ttcagatgca tttcctcaat agcggacaga
     7441 ttgagctaca acttgacgat catcgctact cggtggaagc gcccctgttt gtcctgacgc
     7501 cgccgtcagt acctcatgcg tttattacgg agtctgacgc cgacggtcat gtgttgacgg
     7561 tacgggaaga tctgatctgg cccctgctgg aagttcttta tccgggcact cgggaaacct
     7621 tcggcctgcc ggggatttgc ctgtcactgg cagataaacc cgacgaactg gcggcgctgg
     7681 aacactattg gcaactgata gagcgggaat cggtagaaca actgcctgga cgggaacaca
     7741 ccctgacgtt actggcacag gcagtattca ccctactgct gcgtaatgca aaactcgacg
     7801 accatgccgc cagcggaatg cgcggagaat taaaactgtt ccagcgtttt aatatgctga
     7861 ttgaaagcca ttttcatcag cactggacag taccggatta cgctaacgaa ctgcatatca
     7921 ccgaatcacg cctcacggac atctgccgcc gctttgccaa ccgtccacca aaacggttga
     7981 ttttcgacag gcagctgcga gaagccaaac ggctgctgct gttttctgat aacgcagtga
     8041 acaatattgc ctggcaactc ggttttaaag atccggctta ttttgcccgc ttttttaatc
     8101 gcttagtcgg ttgctcgccc agtgcttatc gcgccaaaaa agtacctgtg acgtgaaaat
     8161 cccttagcct taacgggaaa ccaggcacca cctgctattc ccctttctcc gcctggagca
     8221 aggggattta gtaacgtcaa aaatcgctaa aagcgacctc gatcacaaaa ccgtacccga
     8281 cctgaaaagt accagccatt gtcccaaaag tctcttcccg taatcgcccc tgcaagcttc
     8341 aattgaataa caaaaacaac acacaaaatt aacaatcgta ttccgattaa tactgtagag
     8401 gtcgacatga aaccagaaga tttccgcgcc agtacccaac gtccgttcac cggggaagag
     8461 tatctgaaaa gcctgcagga tggtcgcgag atctatatct atggcgagcg agtgaaagac
     8521 gtcactactc atccggcatt tcgtaatgcg gcagcgtctg ttgcccaact gtacgacgcg
     8581 ctacacaaac cggagatgca ggactctctg tgctggaaca ccgacaccgg cagcggcggc
     8641 tatacccata aattcttccg cgtggcgaaa agtgccgacg acctgcgcca gcaacgcgat
     8701 gccatcgctg agtggtcacg cctgagctat ggctggatgg gccgtacccc agactacaaa
     8761 gctgctttcg gttgcgcact gggcgcgaat ccgggctttt acggtcagtt cgagcagaac
     8821 gcccgtaact ggtacacccg tattcaggaa actggcctct actttaacca cgcgattgtt
     8881 aacccaccga tcgatcgtca tttgccgacc gataaagtaa aagacgttta catcaagctg
     8941 gaaaaagaga ctgacgccgg gattatcgtc agcggtgcga aagtggttgc caccaactcg
     9001 gcgctgactc actacaacat gattggcttc ggctcggcac aagtgatggg cgaaaacccg
     9061 gacttcgcac tgatgttcgt tgcgccaatg gatgccgatg gcgtgaaatt aatctcccgc
     9121 gcctcttatg agatggtcgc gggtgctacc ggctcgccat acgactaccc gctctccagc
     9181 cgcttcgatg agaacgatgc gattctggtg atggataacg tgctgatccc atgggaaaac
     9241 gtactgatct accgcgattt cgatcgctgc cgtcgctgga caatggaagg cggttttgcc
     9301 cgtatgtatc cgctgcaagc ctgtgtgcgc ctggcagtga aattagactt cattacagca
     9361 ctgctgaaaa aatctctcga atgtaccggc accctggagt tccgtggtgt acaggccgat
     9421 ctcggtgaag tggtggcgtg gcgcaacacc ttctgggcat tgagtgactc gatgtgttct
     9481 gaagcgacgc cgtgggtcaa cggggcttat ttaccggatc atgccgcact gcaaacctat
     9541 cgcgtactgg caccaatggc ctacgcgaag atcaaaaaca ttatcgaacg caacgttacc
     9601 agtggcctga tctatctccc ttccagtgcc cgtgacctga acaatccgca gatcgaccag
     9661 tatctggcga agtatgtgcg cggttcgaat ggtatggatc acgtccagcg catcaagatc
     9721 ctcaaactga tgtgggacgc cattggcagc gagtttggtg gtcgccacga actgtatgaa
     9781 atcaactact ccggtagcca ggatgagatt cgcctacagt gtctgcgcca ggcacaaagc
     9841 tccggcaata tggacaagat gatggcgatg gttgatcgct gcctgtcgga atacgaccag
     9901 aacggctgga ctgtgccgca cttgcacaac aacgacgata tcaacatgct ggataagctg
     9961 ctgaaataac gcagcaggag gttaagatgc aattagatga acagcgcctg cgctttcgtg
    10021 acgcgatggc cagcctgtcg gcagcggtaa atattatcac caccgagggc gacgccggac
    10081 aatgcgggat tacggcaacg gccgtctgct cggtcacgga tacaccacca tcgctgatgg
    10141 tgtgcattaa cgccaacagt gcgatgaacc cggtttttca gggcaacggt aagttgtgcg
    10201 tcaacgtcct caaccatgag caggaactga tggcacgcca cttcgcgggc atgacaggca
    10261 tggcgatgga agagcgtttt agcctctcat gctggcaaaa aggtccgctg gcgcagccgg
    10321 tgctgaaagg ttcgctggcc agtcttgaag gtgagatccg cgatgtgcag gcaattggca
    10381 cacatctggt gtatctggtg gagattaaaa acatcatcct cagtgcagaa ggtcacggac
    10441 ttatctactt taaacgccgt ttccatccgg tgatgctgga aatggaagct gcgatttaag
    10501 ctccagcccc ttcacgcatt cgtcgaaggg gcatttttat tgtgatctca atcacatatc
    10561 cacccgatga ataattcttg cccaaccgct caccgccatt cctgaccgct tacgtcccta
    10621 aaataaccac tcagttattt accttacttt acgcgcgcgt aactctggca acatcactgc
    10681 aggatagcgg tcaatttacc tcctcaaacg caacgcaaac ctagaacggc ttcggcctat
    10741 gattaacaaa ttactctatg aattttggtt tgcaggaagg cagcaagtga gtgaatcccc
    10801 gggagcttac aacagtaagt gacaggggtg aacgaacgca actgccgcac ctgtaagcca
    10861 aaagacgacg agtaaaatgc caggttttac tatggatacg aaaaaactat tcaagcacat
    10921 accctgggtg attctcggaa tcatcggtgc attctgcctc gcggtagttg cattacgtcg
    10981 gggggagcac gtcagcgccc tgtggatcgt ggtcgcctct gtgtcggtgt atctggtggc
    11041 gtatcgctac tacagtctgt acatcgccca gaaggtgatg aaactcgacc ccacgcgcgc
    11101 gacgcctgcg gttattaaca acgacggtct gaactacgtt ccgaccaacc gttacgtgtt
    11161 gtttggtcac cacttcgccg ctatcgccgg tgctggtccg ctggtcggtc cggttctcgc
    11221 cgcacagatg ggctacctgc ctggcacgct gtggctgctg gcgggggtag tgctggccgg
    11281 tgcggtacag gactttatgg tgctgtttat ctcctctcgc cgtaacggcg catctctcgg
    11341 tgagatgatc aaagaagaga tggggccagt accggggact atcgcgctgt ttggctgttt
    11401 cttaatcatg atcatcatcc ttgccgtcct ggcgctgatt gtggttaaag ccctggccga
    11461 aagtccgtgg ggtgtcttca ccgtttgctc aaccgtaccg attgcgctgt ttatgggtat
    11521 ctacatgcgc tttatccgtc cggggcgtgt gggtgaagtc tctgtcattg gtatcgtgct
    11581 gctggttgcc tctatctact tcggtggcgt gattgctcac gatccgtact ggggtccggc
    11641 actgaccttt aaagacacca ccattacctt cgcgctgatt ggctatgcgt ttgtttccgc
    11701 attgctgccg gtgtggctga tcctcgcacc gcgcgactat ctggcaacct tcctgaaaat
    11761 cggcgttatc gtcggcctgg cgctgggtat cgtggtgctg aacccggaac tgaaaatgcc
    11821 tgcgatgacc cagtacattg acggtactgg cccgctgtgg aaaggtgctc tgttcccgtt
    11881 cttgttcatt accatcgcct gtggtgcggt atctggcttc cacgcactga tctcttccgg
    11941 tacgacgccg aaactgctgg ctaatgaaac cgacgcgcgt ttcattggct acggcgcaat
    12001 gctgatggag tccttcgtgg cgattatggc gctggttgct gcgtccatca tcgaaccggg
    12061 tctttacttc gcgatgaata ccccgcctgc tggccttggc atcaccatgc ctaacctgca
    12121 tgaaatgggt ggcgagaacg cgccgatcat catggcgcag ttgaaagacg ttaccgcaca
    12181 cgcggcagcg accgtcagct cctggggctt cgtgatttcg ccagagcaga tcctgcaaac
    12241 cgcgaaagac attggtgagc cttctgtcct gaaccgtgca ggtggtgcgc cgacactggc
    12301 ggtaggtatc gcgcacgtgt tccacaaagt gctgccgatg gctgacatgg gcttctggta
    12361 tcacttcggt attctgttcg aagccctgtt catcctgacc gcgctggatg cgggtacccg
    12421 ttctggccgc tttatgctgc aggacctgct gggtaacttc atcccgttcc tgaaaaaaac
    12481 cgactctctg gttgctggta tcatcggtac tgcgggctgt gtgggtctgt ggggctacct
    12541 gctgtatcag ggcgtggtcg atccgctggg cggcgttaag agcctgtggc cgctgttcgg
    12601 tatttccaac cagatgctgg cagccgtagc gctggtactg ggcaccgttg tgctgattaa
    12661 gatgaagcgc acccaataca tctgggtaac tgttgttccg gctgtatggc tgcttatctg
    12721 caccacctgg gcgctgggcc tgaaactgtt cagcaccaac ccgcagatgg aaggcttctt
    12781 ctacatggca agccagtaca aagagaagat tgctaacggt actgacctga cggcgcagca
    12841 gattgccaac atgaaccaca tcgttgtgaa caactacacc aacgcaggcc tgagtattct
    12901 gttcctgatt gtggtgtaca gcatcatctt ctacggtttc aaaacctggc tgaaggtgcg
    12961 taacagcgac aaacgtactg acaaagaaac accgtacgtt ccaatcccgg aaggcggcgt
    13021 gaagatctct tcgcaccact aaccgtgttt agccccgctt cggcggggct ttgttcttat
    13081 cagagtgaac tatgtttggt aacttaggac aggcaaaaaa atatctcggc caggcggcga
    13141 agatgttgat tggcattcca gactacgaca actacgtcga gcatatgaag accaaccatc
    13201 ccgacaagcc gtacatgagc tatgaagaat tcttccgcga acgccagaac gcgcgctacg
    13261 gcggcgatgg taaaggcggt atgcgctgtt gttaatggag agagcatgaa cccgattgca
    13321 gttaccctac tcaccggttt tttaggcgcg ggcaaaacca ccctgctgcg ccatattctt
    13381 aacgaacaac acggctacaa gattgccgtg attgaaaacg aattcggcga agtctccgtt
    13441 gatgatcaac tgattggcga ccgcgccacg cagatcaaaa cgctgaccaa cggctgcatc
    13501 tgctgttcgc gctccaacga gctggaagac gcactactcg acctgctgga taatctcgac
    13561 aagggcaata ttcagttcga tcgtctggtc attgaatgca ccggcatggc cgatcccggc
    13621 ccgattattc agaccttttt ctcccatgag attttatgcc agcgttacct gctggacggc
    13681 gtgattgcgc tggtggatgc ggtacatgcc gatgagcaga tgaaccagtt caccatcgcc
    13741 caatcccagg ttggctatgc cgaccgtatt ctgctgacta aaaccgacgt cgcaggcgaa
    13801 gcagaaaaac tgcgtgaacg tctggcgcgc atcaacgccc gcgcaccggt ctacaccgtc
    13861 acccacggcg acatcgacct gggtctgctg ttcaacacca acggttttat gctggaagaa
    13921 aacgtcgtca gcaccaaacc gcgtttccat tttatcgcgg acaaacaaaa cgatatttcg
    13981 tcgattgtgg tggaactgga ttacccggta gatatcagcg aagtttcccg ggtgatggaa
    14041 aacctgctgc tggaatcggc ggataaactg ctgcgttaca aagggatgct gtggattgac
    14101 ggcgaaccta accgcctgct gttccagggc gtccagcgcc tctacagcgc cgactgggac
    14161 cgcccgtggg gcgatgaaaa accgcatagc acgatggtgt ttatcgggat tcagttgccg
    14221 gaggatgaga ttcggtcagc gtttgcggga ctcaaaaagt aaccagccct ccaacatcac
    14281 aatacctgag actttatcac cctcatattc ctccagaata tgagggcatt cgggcttaat
    14341 tatctctgaa cgtgcaacac ttcctgcgac tccttatgcg cctccacatc ctgcttcgtt
    14401 aaccagagcg acttacgacc aaaattttcg tacattttca gctgatcttc atagtgctta
    14461 tcaactgttc catcggaagc aataaaccca ctctgaccgg gtgcgaccac atcccaggca
    14521 agcaccggac gatcgcttgt cgttggtgag aaaacaatca tatcgttttc tgttccacgg
    14581 ttttgatact ccgcctgatg acgcgtttct tccactgcgg cctgcggtac accaaagaaa
    14641 ttatttgccc ggaacgttaa ggccattgca ggtgttttcc agttactcac attattgcca
    14701 tagcgtttgg aaagagtctc ccaggtatct tccagcgcag ccaacacaac ctcctgctgt
    14761 ggtttcccag caaacagatc aaccgcctgt gggattggtg atttgtctcc ctgcaccgcc
    14821 tcatacaaaa tttttgctcc aacacttata ttcagcgaac cagttgggcc gtcctgggtt
    14881 gtttcgtagc cactggcgct gtaccactta tcaaatggca taggtacggc agccactacg
    14941 gtacgcttca acatactggt cagccaaacg ttcaggatgg cagagcctgg ctgctgccag
    15001 gttttaccat cataattaag caaattgatg ccatcccaac gtgttaatgt atccaccaac
    15061 tgacgacgcg gatcgctctg tgtcaaacca gatgtcgctg cttgcagagt aggtaaaaaa
    15121 agcctcaggt taagatcctt acgactggtt tggcgaataa catcccatgc ctgatcagca
    15181 gttaagcgtg gcttttgctc aagcagtcgg tcgatctccg taacgcgatc tgcccccccc
    15241 acaaaaaggc aaacagatcc gaagcgggat aatctttttg gggagaattg ttccagttag
    15301 caatatatcc cgacaggggg ttatacacct tagggttcat ttcaaaaggc aatagccctt
    15361 tccagtccca ttttcccgta ccaggaacgg gtaatcgcgg atcatggcct gattgacgat
    15421 ctggataagc accagtatga acataaccaa tattgccgtt tacatcagca tagtaccagt
    15481 tgatggtcag tgcttgtttc gctgcctgct gtgtccactc ctgccaattt ttggccttca
    15541 tctgatgagt ccaggccagc aaagacgcca cctctttacc atcccatgcg cgggatttag
    15601 cgtaagccgt ttgtgtcgtc tggtcagttt ggagaatgtt gccatgcacc gtacgccaga
    15661 cagtaaaggt ctctgcctga ccatttttca ccgtaatggt ttcctcacgg cttaacattt
    15721 tcacccactt accattatgc aagtagtagc ctggtttctc tgccgacagc cgttcagcaa
    15781 aaatatcgac atcatcgccg aaacctgccg ttgatcccca ggaaatcaca ccattatgac
    15841 caaaaaccag cccaggatag gcaaatggtg tattgccagt gacatcataa ccagcaccgt
    15901 gcagaccaat accataagta tacgcaggcg cataccagcc aaactgcgga ccatttacca
    15961 tgattgcttt cgcatcctgg gctttgcttt tgccgatcac ccacatattg ctggtcgttg
    16021 gataccccgc cagaccattg gcaccaccct gtgcaaattg tgcagcaata gtttcccggt
    16081 tcttccctgc tgttaacgcc agcagtgcgc catccgcccc ttttgctggt cggtcaagca
    16141 ttggtgcagg taaatcgtag cgtggcaaca gagctgctgt ttgcgagttt tgctgattaa
    16201 atttaagtgg gtagctactc tcttgtacgg caatagtggt tggcgctgat gggtttacca
    16261 gccatttcaa ctgattaaat accgccatgc cttgtgatac accatattta tcttttaaag
    16321 ccgttagcag tgccagatta tcaatttcgc tagtgctatc agagaagcgg tttgccatgg
    16381 tgcccacaaa tatcatcgcg acatcaaacg gttcccagcg cttaggagta aagccaaatg
    16441 tattaaactg ttttggtaag agcgtctctg ggttggtatt taccttatca atccaggcat
    16501 tcattccatc agcgtagcct tgcagaatgg acatatcctc tggggaaagg gcagcaattt
    16561 gcgcccggat agcatccggc cagtagttac gacggatatc tttatcaaat ttcacaaaat
    16621 ctttgccaag cacttccgcg acagttcctt gagtactgcg acgtgccatt tccatctgaa
    16681 aaaggcgatc ttgtgctact acatagccat agccataaaa taggtgccat gtatcattgg
    16741 cataaatatg cggcatgccg tattcatcgc gaacaatctt tatctcactt gacgactgct
    16801 cagccagtgc aggcaagctc caacaacaca tcagggaagc agtaacacag ttcacgatca
    16861 tacgatttct atttttcatt gtatcctctg gcaggtgtat aattagcgaa ttgatactag
    16921 caacgaagct tcggttatct atttgcagag aattgtttca ttatgaactg tgagcgcgat
    16981 aactaatttt tgatacaaaa attgcattaa tgtgatctaa aacgaatcgc tttattcacc
    17041 gctgatgatt ttgttctatt cctttttgtg acagatcccc cggacgcata gatgatgagc
    17101 cccagtgaga cagctcaggt cattatctgt atgtaaatca gaaaaatatg ttgtaaacga
    17161 cttttatggt tggattttgt agcaattttg aatgcagaca ggcagcattt gccaacacgc
    17221 cgcccatagt tcactccaca tcctccctgc gtaacccaat atccctcaac cgttcatcac
    17281 tcatctgctg taacactcgt cgggtctgca tttgccgccc ccaacgcctc accgcctgcc
    17341 agagttgtac gaagccgata aacggcgttt tggctttgtt ttcgtgaaat tccatctgct
    17401 gcctcctcat ctttcaaagg cttcatcatc cgccttctgg cagttcacaa tacagatgca
    17461 aatattagtt ttatttaaca tacagacagg ttaaaacagc atctgcatcc ggttttttag
    17521 cttcatctgt accggttttt ctctctgtat ggtctaaata aggaatacag catgacgcgt
    17581 tatcaacatc tggcaactct gcttgccgag cggattgagc aagggctgta tcgtcacggg
    17641 gagaaattgc cgtcggtgcg cagcttaagt caggagcacg gcgtcagcat cagcaccgtg
    17701 caacaggcgt atcagacgct ggaaacgatg aagctcatca ctctgcagcc gcgttcgggt
    17761 tattttgtcg cacaacgtaa agcccagccg cctgtaccgc cgatgacgcg tccggtgcag
    17821 cgcccggtgg aaattaccca gtgggatcag gtgctggata tgctggtggc gcatagcgac
    17881 agttccattg ttccgttaag caaaagcacg ccggatgtcg aaacgcccag cctgaaaccg
    17941 ctctggcggg agctaagccg ggtggtgcag cataatctgc aaaccgtgct cggttatgac
    18001 ttgttagccg gtcagcgggt attgcgagag cagattgccc gcctgatgct cgacagcggc
    18061 tcggtggtca ccgccgatga catcatcatc accagcggct gccataactc gatgtcgctg
    18121 gcgttaatgg cggtgtgtaa accgggcgat attgtcgcgg tcgaatctcc ctgttattac
    18181 ggttcgatgc agatgctgcg aggcatgggc gtgaaagtga ttgaaatccc aaccgatcca
    18241 gaaactggca tcagcgttga agcactggaa ctggcgctgg aacagtggcc gattaaaggc
    18301 atcattctgg tgccaaactg taataatccg ctgggattta ttatgccgga cgcacgcaaa
    18361 cgggccgttc tctctctcgc tcagcgtcat gatattgtga tttttgaaga tgatgtctac
    18421 ggcgaactgg caacggagta tccgcgcccg cggaccatcc attcatggga tatcgacggg
    18481 cgagtgttgt tgtgcagctc gttcagtaaa agtattgctc caggcctgcg cgtgggttgg
    18541 gtcgcaccgg ggcgctatca cgataaactg ctgcatatga aatacgccat cagcagcttt
    18601 aatgtgccgt ccacgcaaat ggcggcggca acgtttgtgc tggaaggtca ctatcatcgc
    18661 catatccggc ggatgcggca gatctatcag cgcaatttgg cgctttatac ctgctggata
    18721 cgggaatatt ttccctgcga aatctatatt acgcgcccga aaggcggatt tttactgtgg
    18781 atcgaattgc ctgaacaggt cgatatggtc tgcgtcgcgc ggcagctgta ccgcatgaaa
    18841 atccaggtgg cagcaggctc gattttctcg gcttccggca aataccgtaa ttgtctgcgc
    18901 atcaactgcg ctttgccgct tagcgaaacc aatcgcgaag cactaaagca aattggcgat
    18961 gccgtgtatc gggcaatgga atgaattcga attagtggcg aagcacctga tttcctctca
    19021 cgaatcgcct gtgactcgtt ttatctttgc agggtcatcg ccacattcca gcatgggtta
    19081 tatagacatg ataagcgtag cgcaccaggc agctttgcgt ttgtcatcag tctctgatta
    19141 gtactcaagc catgaatcgt gctcacggtc aggcaagggc tgctctaatg cctgcatttg
    19201 ctctgccgaa agtccggtaa tcttttgtat ccattcagga tctacaccat tctgcaaaaa
    19261 taatcgaaga atgcgctgtt ccccttttat acatccatca ttataaattc gctctgcaat
    19321 tgtcattatt cgctccctgt ggtgtggcat tcgcctggta agttcgctga taaacttctt
    19381 aaaacgcgct tcatcgccag tcagtaaaat gtaatttaac agcgctgtta tctggctgtc
    19441 attagcacac tctgtaacca gtaatactac cagttgatcg ataagctcca tcagatcgcg
    19501 ctggcgaata tgcttttgga tcaactctaa cagggcgact ctgcgatgct gcacaatctc
    19561 gtcgtctggc acgacagtga catccaccaa cgggaacgct gcgctataaa gcttccgtgc
    19621 ggtagttggg tcggcaaatt cgtccagcca gcacagggac caggggtaag gactacggct
    19681 accgtgataa aacagcatcg ggatgaccag cggtagctgc cggcgtttat catgctcgat
    19741 atggcgctgc atcactgcca tggaatatcg catcaggcga aaggccatat gaatgtcctc
    19801 gcggctctga tgttcaatca ccacataaat atagccatca ccttcacggg tctttaccga
    19861 ccacagaata tcggagtgta gcgcccgcaa tttttcatcg acaaagctgg cggattccag
    19921 ttttaagctg tcgagatcgc acagttcacg taaatctttg ggtaagtgga tctccataaa
    19981 atcccgcgcg gtgtcagggt gcgtgagaaa ggacttaaat aatgcgtcat gcggcgtgct
    20041 ggttgtgaag tttgtcatgg cgatccgtca cctgctaata agatggcccg actgtagcga
    20101 ctaacaaaac cattctcacc cggcaatttc tcgttccccg aggcactttc caggctattt
    20161 ttgttcgtct gcaaacgaaa catttttccg gaaacaggct cgcgttttac atttcctgta
    20221 ccaggcagat attccccttt ccaccgcgct cttttgatta accgcaaatt ttaattacac
    20281 ttaaggtgta tattttctat gcaacccatc aattcaagag gtgtaatgtg ctgatgacta
    20341 tttgtaatcg ttatacattc tgacctggag tcagaaggta tttctctgtc tgtgtgttca
    20401 caggcagtgt ggttgattac atgaaattca gtacatttgt ccttctcgcc tgcctttcgt
    20461 cattaccgac ggtattgaat ttcgttttcc ccgttggggt tctccggaca aggagttgtt
    20521 tgttatgcca cgtttttttg cccgccatgc cgccacgctg tttttcccga tggcgttgat
    20581 tttgtatgac tttgccgcgt acctgtcgac ggatctgatc cagccgggga tcattaatgt
    20641 ggtccgtgat tttaatgccg atgtcagtct ggccccggca gccgtcagtc tctatctcgc
    20701 tggcggtatg gcgttacagt ggctgctggg gccactttcc gacaggattg gccgcaggcc
    20761 ggtactgatt accggggcgc tcatttttac ccttgcctgc gctgcgacaa tgttcaccac
    20821 gtctatgaca cagtttctta tcgcgcgtgc aattcagggc accagtatct gttttattgc
    20881 taccgttggt tatgtcacgg tgcaggaggc gttcggacag acaaaaggga ttaagttgat
    20941 ggcgattatc acctccatcg tactgattgc gccgattatt ggcccgcttt ccggcgcagc
    21001 tctgatgcac tttgtgcact ggaaagtcct ttttgccatc attgcggtta tgggttttat
    21061 ctcatttgtt ggcttactgc tggcgatgcc tgagacggtg aagcgcggcg cggttccatt
    21121 tagcgccaaa agcgtcttgc gcgattttcg taatgtcttt tgcaatcggc tgttcctctt
    21181 tggcgcaaca accatctctt taagctatat cccaatgatg agttgggtgg ctgtctcgcc
    21241 ggtgatcctg attgacgcag gcggcttaac aacttcgcag ttcgcctgga cacaggttcc
    21301 ggtgttcggc gcggtgattg tcgcgaatgc catcgtggcg cgtttcgtta aagatccgac
    21361 cgaaccgcgc tttatctggc gtgccgtacc cattcaactg gttggcctcg cgctgttgat
    21421 tatcggcaat ctgctgtcac cgcacgtctg gctgtggtcg gtgctgggca ccagtttgta
    21481 tgctttcggg attggtttaa tcttcccgac cttattccgc tttacgcttt tttccaataa
    21541 cttaccgaaa ggaaccgtct ccgcatcgct gaatatggtg atcctgatgg tgatgtctgt
    21601 ctcagtcgaa atcggccgct ggctgtggtt taacggcggt cgcttgccgt ttcatctgtt
    21661 agccgtcgtg gcgggcatta tcgtcgtttt caccctggca ggattgctta accgcgtgcg
    21721 ccaacatcag gctgccgagc tggcggagga gcggtgattt ttgcgcgatc ctgccgtcag
    21781 gctctattct taacgttatg aataaactca ttgaactcag acgcgccaaa atgttggcgc
    21841 tctctttact gcttatcgcc gctgctacct ttgtcgttac gctgtttttg ccgcccaatt
    21901 tttgggtgag cggcgtgaag gcgattgctg aagcggcgat ggtcggcgcg ctggcggact
    21961 ggtttgcggt ggtggcgctg tttcgccgcg tgccgattcc gatcatttct cgtcatacgg
    22021 cgattatccc gcgtaataaa gaccggattg gcgaaaatct cggccagttc gtgcaggaaa
    22081 aatttctcga tacccagtcg ctggtggcat tgattcgacg tcacgaaccg gcgttgttga
    22141 ttggcaactg gtttagtcag ccagaaaacg cccgccgcgt tggtcaacat ctgttgcaga
    22201 tcatgagcgg ttttcttgaa ctgaccgatg atgcgcgtat tcagcgcttg cttaagcgcg
    22261 cggtccatcg ggcgattgat aaggtcgatc tttccggcac cagtgcgttg atgctggaga
    22321 gtatgaccaa aaacgaacgt catcaggtgc tgctggatac gctgatcgca cagttgatcg
    22381 cccttctcca gcgcgataaa tcgcgcaagt ttatcgccca gcaaattgtt cgctggctgg
    22441 agagcgagca tccactgaaa gccaaaattt tgcccactga atggctgggc gaacatagcg
    22501 cggagttggt ttctgacgcg gtgaattctt tgcttgatga tatcagccgc gatcgtgcgc
    22561 atcagatccg tcatgcgttt gatcgcgcca cttttgccct gatcgacaag ttgaaaaacg
    22621 atccggaaat ggcagcgcga gccgatgccg taaaaagtta tctgaaagaa gatgaagctt
    22681 ttaaccgcta tctcagtgaa ttgtgggggg atttacggga atggctgaaa gcggatatca
    22741 acagtgaaga ttctcgtgtg aaagaacgta tcgcgcgggc gggtcaatgg tttggcgaaa
    22801 cgttaattgc cgatgatgcc ttgcgggcgt cgttaaatgg tcacctggaa caagccgcgc
    22861 accgcgtcgc gcctgagttt tccgcattcc tgacgcgcca catcagcgat acagtaaaaa
    22921 gctgggatgc gcgggatatg tcgcggcaaa tcgagttaaa tatcggcaaa gatctgcagt
    22981 ttatccgtgt caacggtacg ctggttggcg gttgtattgg gctaatttta tatttgttgt
    23041 cgcagctccc ggccttgttc cccctcggca atttttagaa atccatgaaa aactaagtgg
    23101 ctaaccgcga gagagatcaa ataaaccaca ctaaaagaat ggtaataatg tggcctcttt
    23161 tcattatctc ccgtggtacg gggaaggaaa atcatgtcac ttatcaccga tctacccgcc
    23221 attttcgatc agttctctga agctcgccag aaaggctttc tcaccgtcat ggatctcaag
    23281 gagcgcggca ttccgctggt tggcacttac tgcaccttta tgccgcaaga gatcccgatg
    23341 gcagccggtg cggttgtggt ttcgctctgt tctacctctg atgaaaccat tgaagaagcg
    23401 gagaaagatc tgccgcgcaa cctctgcccg ctgattaaaa gcagctacgg cttcggcaaa
    23461 accgataaat gcccctactt ctacttttcg gatctggtgg tcggtgaaac cacctgcgac
    23521 ggcaaaaaga aaatgtatga atacatggcg gagtttaagc ccgttcatgt gatgcagttg
    23581 ccgaacagcg ttaaggacga tgcctcgcgt gcgttatgga aagccgagat gctgcgcttg
    23641 caaaaaacga tagaagaacg ttttgggcac gagattagcg aagatgctct gcgcgatgcc
    23701 attgcgctga aaaaccgcga acgtcgcgca ctggccaatt tttatcatct tgggcagtta
    23761 aatcctccgg cgcttagcgg cagcgacatt ctgaaagtgg tttacggcgc aaccttccgg
    23821 ttcgataaag aggcgttgat caatgaactg gacgcgatga cagcccgcgt tcgtcagcag
    23881 tgggaagaag gccagcggct ggccctgcgt ccacgcattt taatcaccgg ctgcccgatt
    23941 ggcggcgcag cagaaaaagt ggtgcgcgcg attgaagaga atggcggctg ggttgtcggt
    24001 tatgaaaact gcaccggggc gaaagcgacc gagcaatgcg tggcagaaac gggcgatgtc
    24061 tacgacgcac tggcggataa atatctggcg attggctgct cctgtgtttc gccgaacgat
    24121 cagcgcctgc aaatgctcag ccagatggtg gaagaatatc aggtcgatgg cgtagttgat
    24181 gtgattttgc aggcgtgcca tacctacgcg gtggaatcgc tggcgattaa acgtcatgtg
    24241 cgtcagcagc acaacattcc ttatatcgct attgaaacag actactccac ctcagatgtc
    24301 gggcagctca gtacccgtgt cgcggccttt attgagatgc tgtaaggagt ggcagtgaca
    24361 tattcgattg gcattgattc tggttctacc gccaccaaag ggatcttact ggcagacggc
    24421 gtgattacgc gccgtttcct cgttccaaca ccctttcgtc cggcaacagc aattactgaa
    24481 gcctgggaaa ctctgcgcga agggttagag acaacgccgt ttctgacgct caccggctat
    24541 ggacggcaac tggtggattt tgccgataaa caagtgacgg aaatctcctg tcatgggctg
    24601 ggcgcacggt ttcttgcgcc agcaacgcgc gcggtaatcg acatcggtgg tcaggacagc
    24661 aaagtgattc agcttgatga tgacggtaac ctgtgcgatt tcctgatgaa tgacaaatgc
    24721 gcggcgggca ccgggcgttt cctggaggtg atctcgcgca cgcttggcac cagcgtcgag
    24781 caactcgaca gcattaccga aaatgtcacg ccgcacgcca tcacgagtat gtgcacagtg
    24841 tttgccgaat cagaagtgat cagcctgcgc tcagcgggcg tcgcgccaga agcgattctc
    24901 gccggagtga ttaacgcgat ggcgcggagg agtgccaatt tcattgctcg tctctcctgt
    24961 gaagcgccga ttctgtttac tggtggcgtt agtcattgcc agacgtttgc ccggatgctg
    25021 gaatctcatc tgggaatgcc ggtaaatacc catcctgatg cgcaatttgc tggcgcaatt
    25081 ggcgcggcgg taattggtca acgagtgagg acacgccgat gaaagagttt ttatttttat
    25141 ttcactccac agtcggcgtc atacaaaccc gcaaagcgtt gcaggcagcg ggcatgactt
    25201 ttcgcgtcag cgatattccc cgtgatttac gcggcggctg cgggttatgc atttggctga
    25261 cctgtccccc cggcgaggaa atacaatggg tgatccctgg gctgactgag tcaatttatt
    25321 gtcagcagga tggtgtctgg cgctgcattg cacattaccg ggtttctccg cgttaaggat
    25381 tcattaaggt cgctacgtat acttctcgat atcttattga tttgccttaa agggattacc
    25441 gtgacaaaaa gtatttcgtt aagtaaacgc atctccgtta ttgtgattct ttttgccatt
    25501 gtggcagtgt gtactttctt cgtgcaatct tgcgcccgta aaagcaatca tgcagcatca
    25561 tttcagaatt accacgccac cattgacggc aaagaaattg caggcataac gaataatatc
    25621 tcttcgctga cctggtcggc acaaagcaat actcttttta gcactatcaa caaacctgcc
    25681 gccattgttg aaatgaccac caatggtgat tttatccgca caattcctct ggattttgtt
    25741 aaggatctcg agacgatcga atacattggc gataatcagt ttgtcattag cgatgagcgc
    25801 gattacgcca tttatgtgat ttcactgacc ccaaactcgg aagtaaaaat cctcaaaaaa
    25861 ataaaaatcc cgctgcaaga ctccccaacc aattgcggtt ttgaagggtt ggcttattct
    25921 cgccaggatc acactttctg gttttttaaa gagaaaaatc cgattgaggt ctacaaagtt
    25981 aacgggctgt taagtagcaa tgaattacac atcagcaaag acgaggcact acaacggcaa
    26041 tttactttag atgatgtgtc cggcgcagaa tttaaccagc aaaaaaatac gcttctggtg
    26101 ctgtctcatg aatctcgcgc actccaggaa gtaaccctgg ttggcgaggt tatcggtgaa
    26161 atgtcactaa ccaaaggcag tcgaggtttg tcgcataaca tcaaacaggc cgaaggggtt
    26221 gcgatggatg cctctggtaa tatttatatc gttagcgaac caaatcgttt ttatcgcttt
    26281 acccctcaat catcgcatta acaggctggc atgcttcacc tttttgttat taacttgtta
    26341 acaacaaaaa tgacaaacaa ggttaaacat gccttcgtcc acgcatcccg tagaacgctt
    26401 ttctttcagc accgcgcttt tcgggatgct ggttctgacc ttaggtatgg gtttaggccg
    26461 cttcctttat acgcctatgt tgcccgtcat gatggcggaa ggatcgtttt catttagcca
    26521 gctctcgtgg attgccagcg gcaactatgc ggggtatctg gctggcagtc tgctattttc
    26581 gtttggcgca tttcaccagc cgtcgcgcct acgcccattt ctgttggttt ctgccctggc
    26641 gagcgggttg ttgatcctcg caatggcatg gttgccgcca tttattctgg tgttattgat
    26701 tcgcttcctg gcgggtgtcg ccagcgccgg tatgctgatt tttggttcga cgctgattat
    26761 gcagcacacg cgccatcctt ttgtgctggc agctttgttt tctggcgttg gcattggcat
    26821 tgcactgggc aacgaatatg ttctggcagg cctgcatttt gccttatcct cgcaaacgtt
    26881 atggcaaggc gccggggcgc tttctggcat gatgctgatt gcacttacgc ttttaatgcc
    26941 ctcgaaaaaa cacgccatcg caccaatgcc attggcaaaa acggagcaac agataatgag
    27001 ctggtggtta ctggctattc tgtacggcct ggcgggattt ggttatatca tcgtcgcgac
    27061 ttacctgccg ctcatggcga aagatgcagg ctcaccattg ttaaccgccc atctctggac
    27121 gttagtcggc ttatcaattg tgccaggctg ctttagctgg ctatgggcag caaaacgttg
    27181 gggggctttg ccttgcctga cggcgaattt gctggtgcag gctatcagtg tgctgcttac
    27241 tctcgccagc gactctcctc tcttgcttat cattagtagt atcggttttg gcggtacctt
    27301 tatgggcacg acatctctgg tgatgactat cgcccgccaa ctgagcgttc cgggaaatct
    27361 caatctttta ggctttgtga cgctcattta cggcattgga caaatcctcg gcccggcgct
    27421 gaccagtatg ctcggcaatg gaacatcggc gcttgccggc gccactctct gcggcgcggc
    27481 ggcgttgttt attgctgcgc ttatcagcac agtacaatta tttaagctgc aagtggtcac
    27541 ttcatgagga gtgtttccct taccactcaa gaaattcgac cgcaaccgtt gatgtcagcc
    27601 agactccgtt ttccgcttgc cagaaaggga tgcctcgttt cgccatttcc tgtgctttga
    27661 cggttaagat aaccggcgat ccgtgtcttg ctcccacttt gcgtgccgtt gcttcatcag
    27721 cagagagatg cacgtaatga cgctcccctg caatcagccc ctgttttttt atctcgtcaa
    27781 ggaaacgact tgccgtaccg tggtaaagaa actgtggcgg tgttttttcg gcaaaggaaa
    27841 tagcgacctg cgaagtcgaa tgcccctgaa ccgcgcggat gcacctacca tcactggaat
    27901 aactaaaacg ctttttatcg ctggtggcaa ccacagtatc cagcaatgca cgggttaacc
    27961 gcttgcccgc tttttgggca caaagtatga gcttatcgat atccgcccat ccttcacggt
    28021 ccagtacgat accaatcgcc tccggtttgt gtcggaggac aaaacttaaa aacttgctcg
    28081 tatccgccag ctctttttcg ttgtactttg ccatctcgtt ttcgtccctg gattttctgt
    28141 ttttatttac tgatgtcatt gtcacttggt ttatgaattt tctgcaactc aatcctgcgc
    28201 agagcctttt ccacacagcg taatttttcc gctatgcaaa aaacgcatag ccagaaacat
    28261 acttgcattt tccccatacc cacatggctg ataaggtttt tcagttccag gaattttcct
    28321 ggctcaagca acacacaaac acaaaaaaac aatggggatt gttatgacac aacaagggga
    28381 tgctgtagcc ggggagctgg cgacagaaaa agtgggcatt aaagggtatc tggccttttt
    28441 tctcaccata atcttttttt ccggtgtttt ttccggaaca gacagctggt ggcgcgtttt
    28501 tgatttttcc gttctgaacg gatcgtttgg gcaactgcct ggtgcgaacg gagccaccac
    28561 ctcatttcgc ggcgcgggtg gcgcgggggc taaagatggc tttctctttg cgctggagct
    28621 ggcaccgtca gtcattctct ctctggggat tatttcaatt accgatggtc ttggtggatt
    28681 acgcgccgcg cagcagttaa tgacacctgt gctaaaaccg ttgttgggca ttccagggat
    28741 ctgctcgctg gcgctgatcg ctaacctgca aaacaccgat gccgccgccg gtatgaccaa
    28801 agagctggcg caggaaggtg aaattaccga acgcgataaa gtgattttcg ccgcatatca
    28861 aaccagcggt agcgccatca tcaccaacta cttctcttcc ggcgtcgcgg tgtttgcctt
    28921 tctgggcacg tccgtaatcg tacctttagc cgtcatcctg gtgtttaaat ttgtcggagc
    28981 caacattttg cgcgtctggc ttaacttcga agaacgtcgc aacccaacgc aaggagcgca
    29041 agcatgacaa ctcaggtccg caaaaacgtc atggatatgt ttatcgatgg cgcgcgccgt
    29101 ggctttacta tcgccacgac caacctgctg ccaaacgtgg tgatggcgtt tgtcatcatt
    29161 caggcgctga aaattaccgg cctgctcgac tgggtggggc atatctgtga acccgtaatg
    29221 gcgctgtggg gattacccgg tgaagcggca accgtccttt tagccgcatt aatgagtatg
    29281 ggtggcgcag tcggtgttgc cgccagtctg gcaaccgctg gcgcattaac cggtcacgat
    29341 gtcaccgttt tactgcccgc gatgtattta atgggcaacc cggtgcaaaa tgtcggtcgc
    29401 tgcctgggga cagccgaagt gaatgccaaa tattacccgc atatcattac ggtctgcgtg
    29461 attaacgcat tgctgtcgat ctgggttatg cagcttattg tttaacaagg agttaccatg
    29521 attgattata ccgcagccgg ttttaccctg ctgcagggag cgcatttgta tgcgccggaa
    29581 gatcggggaa tttgcgatgt cctcgtcgct aacggcaaaa ttatcgccgt tgccagcaat
    29641 atcccttctg acattgtacc gaactgcacg gttgtcgatc tcagtgggca gatcctctgc
    29701 ccaggtttta ttgatcaaca cgtccatttg attggcggtg gcggcgaagc aggtcccacg
    29761 acgcgcacgc cggaagtggc gctaagtcgc ctgacggaag cgggcgtcac gtcagtggtt
    29821 ggtctgctgg gcaccgactc tatctctcgc cacccggaat ccctgctcgc caagacccgt
    29881 gcgctcaatg aagaaggcat cagcgcctgg atgctgaccg gcgcttatca tgtcccttcc
    29941 cgcaccatta cgggttccgt ggaaaaagac gtggcgatta tcgatcgtgt gattggcgtg
    30001 aaatgcgcca tctctgatca ccgttctgcc gcaccggacg tttatcacct ggccaatatg
    30061 gcggcagaat cccgcgttgg cggtttgctc ggcggtaaac ctggcgtcac cgtgttccac
    30121 atgggcgaca gtaaaaaggc gttacagcct atttatgacc tgctggaaaa ctgcgatgtg
    30181 ccgatcagca agctgctgcc gacccacgtt aaccgcaacg taccgttgtt tgagcaggcg
    30241 ctggagttcg cgcgcaaagg cggcaccatc gatatcacca gcagcattga cgaaccggtc
    30301 gcccctgccg aaggtattgc ccgcgccgtt caggcgggta ttccgctggc acgcgtcacc
    30361 ctcagctccg acggcaacgg tagccagccg ttcttcgatg acgaagggaa tttaacccat
    30421 atcggtgttg ccggttttga aacgttgctg gaaaccgtgc aggtgctggt caaagactat
    30481 gatttcagta tcagcgatgc cctgcgcccg ctcaccagta gcgtagccgg tttccttaac
    30541 ctgaccggga aaggcgaaat tctgccaggc aatgatgctg acttgctggt catgacgcca
    30601 gaactgcgca ttgagcaggt atacgctcgc ggcaaactga tggtcaaaga cggcaaagcc
    30661 tgcgtgaaag gaacgtttga aacggcttaa acggttgtca gccgcccgaa tggacgtaga
    30721 atgcccgcaa gggcggctga cagagtaaaa cgtaatggat gactgtggtg cgattttgca
    30781 taatattgaa accaaatggc tttatgattt tctgaccctg gaaaaatgcc gcaatttttc
    30841 ccaggcggca gtcagtcgca acgtctcgca accggcattc agccgccgca tccgtgcgct
    30901 ggaacaggcg attggtgttg aattgtttaa ccgccaggta acgccgctgc aactctcaga
    30961 acaagggaaa atcttccatt cgcagatccg ccatctgttg caacagttag agagcaatct
    31021 ggcagagctg cgtggcggca gcgattacgc gcaacgaaaa atcaagatag ccgctgcaca
    31081 ctctctttcc ctcgggctgt taccgtccat tatcagccag atgccgccgc tctttacctg
    31141 ggcaattgaa gctattgatg tcgatgaagc ggtcgataaa ctgcgtgaag ggcaaagtga
    31201 ctgtattttt tcctttcacg acgaagattt gctggaagcg ccgtttgacc acattcgctt
    31261 atttgaatct caattgttcc ctgtctgcgc cagtgacgaa cacggagaag cactttttaa
    31321 cctcgcgcag ccacactttc cgttactgaa ttacagccgc aactcctaca tggggcgatt
    31381 gattaatcgc accctgacgc gccacagtga gttaagtttc agcacctttt ttgtctcttc
    31441 gatgagcgag cttttaaagc aggttgccct cgacggctgt gggattgcct ggctgccgga
    31501 gtacgccatc caacaagaaa ttcgcagcgg gaagctcgtt gtactgaatc gggacgaact
    31561 ggtgatcccg attcaggcct atgcataccg aatgaatacc cgcatgaatc cagttgccga
    31621 acgcttctgg cgtgaactgc gcgagctgga gattgtgctt agctgacatt ctccagcatc
    31681 gcactacacc aaccgccatt atcccagacc agatcaaacg ttagcggttc accgtcgtgg
    31741 tacagcatta tctcaaggct gatcgccaga atgtggcagt acgcttcttt aaaattaacc
    31801 tgcaacgccg caatccgtgg ctcatgaaat aacagtgcag aacgcagctg acggcaatac
    31861 cagctctgga gatgtttgtc gctctggtta tgcacttgcc actctggcat gcctgaaaca
    31921 agtaatgacg gtaatgccga atgaggagaa ttaaaaagag tgaggagatc gctgcatacc
    31981 gatttacgca gcaatgacgt tttattgcct gaaatttcag gtaaaacagc ctgaagtgat
    32041 tggcgcatca ttttgcgcac tcctgacgtt tagcaacata tgcggcttgc cctgaacacc
    32101 gggcaccact gtattgatgc tcattttcca tagccattcc cttttgttca ttacatccat
    32161 gtcagtctga gacaggcctg aatcctgtca gcaaagtata aaaccttact tgcctatgaa
    32221 tatctactga cttttcagga aaaaagtcat gatttgataa aaatcatcaa actcaaaaaa
    32281 aaaccgctag tatattctga atattccgaa tagttacgta gatgaaaata cttgtcaccg
    32341 ctgctactta attccggtgg gaaattattt atttccgcat aatcagccat gtatt
//
LOCUS       DAJOWB010000044        31811 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJOWB010000044 DAJOWB010000000
VERSION     DAJOWB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.044"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(201..1574)
                     /gene="argH"
                     /locus_tag="OHO85_002223"
     CDS             complement(201..1574)
                     /gene="argH"
                     /locus_tag="OHO85_002223"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCQ2671590.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1635..2411)
                     /gene="argB"
                     /locus_tag="OHO85_002224"
     CDS             complement(1635..2411)
                     /gene="argB"
                     /locus_tag="OHO85_002224"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCQ2671591.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(2419..3423)
                     /gene="argC"
                     /locus_tag="OHO85_002225"
     CDS             complement(2419..3423)
                     /gene="argC"
                     /locus_tag="OHO85_002225"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCQ2671592.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3577..4728
                     /gene="argE"
                     /locus_tag="OHO85_002226"
     CDS             3577..4728
                     /gene="argE"
                     /locus_tag="OHO85_002226"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCQ2671593.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            5080..7731
                     /gene="ppc"
                     /locus_tag="OHO85_002227"
     CDS             5080..7731
                     /gene="ppc"
                     /locus_tag="OHO85_002227"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCQ2671594.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7914..9647
                     /locus_tag="OHO85_002228"
     CDS             7914..9647
                     /locus_tag="OHO85_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCQ2671595.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9796..10647
                     /locus_tag="OHO85_002229"
     CDS             9796..10647
                     /locus_tag="OHO85_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2671596.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10634..10975)
                     /locus_tag="OHO85_002230"
     CDS             complement(10634..10975)
                     /locus_tag="OHO85_002230"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCQ2671597.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10977..11855)
                     /locus_tag="OHO85_002231"
     CDS             complement(10977..11855)
                     /locus_tag="OHO85_002231"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCQ2671598.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11821..14118)
                     /locus_tag="OHO85_002232"
     CDS             complement(11821..14118)
                     /locus_tag="OHO85_002232"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCQ2671599.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14169..14489)
                     /locus_tag="OHO85_002233"
     CDS             complement(14169..14489)
                     /locus_tag="OHO85_002233"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCQ2671600.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVANADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14504..15583)
                     /locus_tag="OHO85_002234"
     CDS             complement(14504..15583)
                     /locus_tag="OHO85_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCQ2671601.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLISRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15892..18393
                     /gene="ptsP"
                     /locus_tag="OHO85_002235"
     CDS             15892..18393
                     /gene="ptsP"
                     /locus_tag="OHO85_002235"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCQ2671602.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDKAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18405..19067
                     /gene="fsa"
                     /locus_tag="OHO85_002236"
     CDS             18405..19067
                     /gene="fsa"
                     /locus_tag="OHO85_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCQ2671603.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            19078..20181
                     /gene="gldA"
                     /locus_tag="OHO85_002237"
     CDS             19078..20181
                     /gene="gldA"
                     /locus_tag="OHO85_002237"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCQ2671604.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            20456..21073
                     /locus_tag="OHO85_002238"
     CDS             20456..21073
                     /locus_tag="OHO85_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCQ2671605.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21100..22005)
                     /gene="yijE"
                     /locus_tag="OHO85_002239"
     CDS             complement(21100..22005)
                     /gene="yijE"
                     /locus_tag="OHO85_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCQ2671606.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRAMRPTPFKYTLAIALLQTCGMIGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALVYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGVVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22099..24279)
                     /gene="katG"
                     /locus_tag="OHO85_002240"
     CDS             complement(22099..24279)
                     /gene="katG"
                     /locus_tag="OHO85_002240"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCQ2671607.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24608..25498)
                     /gene="metF"
                     /locus_tag="OHO85_002241"
     CDS             complement(24608..25498)
                     /gene="metF"
                     /locus_tag="OHO85_002241"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCQ2671608.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25847..28279)
                     /gene="metL"
                     /locus_tag="OHO85_002242"
     CDS             complement(25847..28279)
                     /gene="metL"
                     /locus_tag="OHO85_002242"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCQ2671609.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28282..29442)
                     /gene="metB"
                     /locus_tag="OHO85_002243"
     CDS             complement(28282..29442)
                     /gene="metB"
                     /locus_tag="OHO85_002243"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCQ2671610.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29719..30036
                     /gene="metJ"
                     /locus_tag="OHO85_002244"
     CDS             29719..30036
                     /gene="metJ"
                     /locus_tag="OHO85_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCQ2671611.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30220..30828
                     /locus_tag="OHO85_002245"
     CDS             30220..30828
                     /locus_tag="OHO85_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCQ2671612.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            30973..31648
                     /locus_tag="OHO85_002246"
                     /pseudo
     CDS             30973..31648
                     /locus_tag="OHO85_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         7691 a         8849 c         8056 g         7215 t
ORIGIN      
        1 tggggagtgt gaaggtaaag aagacacaat gataaaaagg aatgtgacaa ataatggttc
       61 tggttgggcg ttaaatttaa gctcaccaga atgctgacaa atttctgcct gatgtggcgt
      121 aaacgcctta tccgcctacg aagtgtcgca ctttgtaggc gtggtaagct ccccattttc
      181 aggggagcaa tataaacaaa ttactctaac cgcgcctgcg caaaagcaat tgcctgcgcc
      241 acctgctgcg gtgagacgcc gccttttgcc gcacgcttgt cgaggcacga ttgcagcgac
      301 agaatcggat agacatcttc gtcaatcacc gggctgaatt tctgcaactc gtcgagcggc
      361 agatcttcca gcggtttgcc ctgacgaatg gcttccacca ccgcttcacc aacaatatgg
      421 tgcgcctcgc ggaacggtac gcctttcgcc accagataat ccgccagttc ggtggcgttg
      481 gcgtaaccct gctgagccgc ttcctggcaa cgtggacgtt tcacctgaat gccgtccagc
      541 accagcgccg ccatatgcag gcagtccagc caggtatcga gcgcgtcgaa cagaccttct
      601 ttgtcttcct gcatatcttt gttgtaagcc agcggcaaac ctttcagcgt catcatcatg
      661 ccggttaacg ccccctgcac ccgaccgcat ttaccgcgaa tcagctccag cgcatccggg
      721 tttttcttct gcggcattaa tgatgaaccg gaagtcacgc ggtcagaaag ctccacaaac
      781 cccgcttcgc cggtgttaaa gaaaatcaga tcttcagcaa aacgcgacag atgcaccatg
      841 ccgatagcgg cagcagaaag cagttccaac acatggtcac ggtcagaaac gctgtcgaga
      901 ctgttacggg tcgccgaagc aaagcccagc cagcctgcta actgttcacg gtcgatttca
      961 taggccgttc ccgccagcgc gccacagcct agcgggctga catccagacg cttaagcgca
     1021 tcctgcaaac ggctttcatc acgcgccagc atctcaacat aggccaggca ccagtgcgcg
     1081 aacgtcaccg gctgggcgcg ttgcaggtga gtgtaacctg gcattaccgc gtcctgattg
     1141 ttttgtgcgg tttccaccag cgccgattgc agctgccggt tagccgtcag taactcgcta
     1201 acggtatctt tgcaccacag tttcaggtca gtcgctacct gatcattacg gctacgcccg
     1261 gtatgcagct ttttgcctaa ctggcccact ttgtcgatca gtttgccttc cacccagcta
     1321 tggatatctt cggcgtcgct ttcaaggatt tgttgtggcc tggcgcgaac atcttccagc
     1381 aacacgttca gcgcctcttc cagttgcgcc tgctcttctg cggttaacac gcctaccgtg
     1441 accagggctt tggaccaggc cacagagcca acaatatcct gctccgccag acggtaatca
     1501 aagcgcagtg agtcgttgaa ttgtttgaac cgttgatctg ctgcctgggt aaaacgcccg
     1561 ccccaaagtg ccataactct gtttccttat tttgaaattc aatgccggaa agctgcgcct
     1621 ccggccaaca aaacttaagc taaaatccgc gtacccatcg gcataccgtt aaacagtgcc
     1681 ggaagctgct ccgcatgacg ccaggaggcg atatctaccg gacggcccag cgtgcgggcc
     1741 gcatccagcg ccgcgttcac tttcactatc atgccgtcag taataatgcc ctgctcaatc
     1801 agttgttctg ctttcgcggc ggtcatttcg gcaatgcgtt gccctttgcc gtcgagaatg
     1861 ccgctgacgt cggagagcaa aatcagatcc gcgcccagcg ttgccgccag cgccgttgcc
     1921 gcctggtcgg cattgacgtt catcagttgc ccttcgtctg ttacgccaat ggagctgacc
     1981 accggcagat aaccgttctc cagcaaggag ttgataagct taggcgaacc tggctgcgcc
     2041 agtccaacat gacctaactc ttcatcaagc tgggtcactt tgacgctgtc gccgtcaccg
     2101 agaaacaaac ctacggccgc aatctgatgt ttcttcgccc atgccaacag ggttttattt
     2161 gccgttcccg ccagtgctcc ggtgataatg tctatctgat cagcaggcgt cacccgcagg
     2221 ccgtttttct ttttcaccgg cagattcagc cctttcatca gctcatccac cacgcaaccg
     2281 ccgccgtgca caatcaccag cggacgctga tgtgactcac gataattcac cagtgcgcta
     2341 aacagacgtt ccagcgcctc ttcactatcc agcagtacgc cgcccagttt gataattaat
     2401 ggattcatca ttgcaccctt aaataagaga ctgcgtttcc gcatagccga aacgaatatt
     2461 ggcgcactgt accgcttgtg ccgccgcgcc tttcagtaag ttgtcttcgg tcgccacaat
     2521 aatcagatgc tcgccctgaa cggcaaaccc gatatcgcaa aatggcagcc caacgacatt
     2581 tttcagcgcc ggaacgcctt tgtcatacag ccgcaccagc ggtttatggg catacgcctg
     2641 ctgtaacact tgcgcgactt gcgcctgggt cacacccgat ttcaggcggc aggtaatggt
     2701 ttcgagaatg ccgcgcggga aattgcccag atgtggggtg aagataacgt cagcaccgag
     2761 gtgtgtggcg atctctggtt gatggcgatg agtaaagacg ccatacggtt gcaggctaac
     2821 ttcacaaaag ctgtttgaaa tggccgcttt acgccctgca ccgctcacgc cgctggtggc
     2881 gttgatcacc ggccactgat tgaggtcaag aagatcggca tcaatcaacg gtttcagcgc
     2941 cagctgtgcc gccgtcggat aacagcccgg caccgcaatc aaattcgctt cttttaattt
     3001 attaccgcac cactccgcca gaccgtaggc tgcctgttcc aacagttccg ggtattgatg
     3061 ggtaaagccg taatattttt catagaaggt ggcgtcgtta acacgaaacg cgccggaaag
     3121 gtcgaacacc acgcagcccg cttcaagaaa ctgcggcgct aaatcgtggc taacttcatg
     3181 ggcggtggcg agaaacacta cgtccacccc tgggctaaac tcgctgatat ccgacatcgg
     3241 ctgcaacggc agatcaacga tgccttttag ctgcggatgc aaatcggaga ttaactttcc
     3301 cgcatcattg ctttgcgctg aaacagtcaa agcggttatg ttcatatgcg gatggcgatt
     3361 tacataggtc actagctctg cgccagcgta gccgctggca cccacaatca gcgtattcaa
     3421 catcggggct attcaccttc ttatgtctgg ttgccaggtt aaacgtaaaa cattcacctt
     3481 acggctggtg ggttttatta cgctcaacgt tagtgtattt ttattcataa atactgcatg
     3541 aatattgata ctatcatgac cagaggtgtg tcaacaatga aaaacaaatt accgccattt
     3601 atcgagattt accgcgctct gattgccaca ccttcaataa gcgccacgga agaggcactc
     3661 gatcaaagca atgcagattt aatcactctg ctggcggact ggtttaaaga tttgggcttc
     3721 aatgtggaag tgcagcctgt tccaggaact cgcaacaaat ttaatatgct ggccagttgc
     3781 ggacaggggg ctggcggctt gttgctggcg gggcataccg atacggtgcc atttgatgac
     3841 ggtcgctgga cgcgcgatcc gtttacactg acggagcatg acggcaagct ttacggctta
     3901 ggcaccgccg acatgaaagg cttttttgcg tttatccttg atgcgctacg cgatgtcgac
     3961 gtcacgaaac tgaaaaaacc gctctacatt ctggcgactg ctgatgaaga aaccagtatg
     4021 gccggagcgc gttattttgc cgaaactacc gccctgcgcc cggattgcgc catcattggc
     4081 gaaccgacgt cactacaacc ggtacgcgca cataaaggtc atatctctaa cgccatccgt
     4141 attcagggcc agtcggggca ctccagcgat ccagcacgcg gagttaacgc tatcgaacta
     4201 atgcacgacg ccatcgggca tattttgcaa ttgcgcgata acctgaaaga acgttatcac
     4261 tacgaagcgt ttaccgtgcc ataccctacg ctcaacctcg ggcatattca cggtggcgac
     4321 gcttctaacc gtatttgcgc ttgctgtgag ttgcatatgg atattcgtcc gctgcctggc
     4381 atgacactca atgaacttaa tggtttgctc aacgatgcat tggctccggt gagcgaacgc
     4441 tggccgggtc gtctgacggt cgacgagctg catccgccga tccctggcta tgaatgccca
     4501 ccgaatcatc aactggttga agtagttgag aaattgctcg gagcaaagac cgaagtggtg
     4561 aactactgta ccgaagcgcc gtttattcaa acgttatgcc cgacgctggt gttggggcct
     4621 ggctcaatta atcaggctca tcaacctgat gaatatctgg aaacacggtt tatcaagccc
     4681 acccgcgaac tgataaccca ggtaattcac catttttgct ggcattaaaa cgtaggccgg
     4741 ataaggcgct cgcgccgcat ccggcactgt tgtcaaactc cagtgccgca ataatgtcgg
     4801 atgcgatact tgcgcatctt atccgaccta cacctttggt gttacttggg gcgatttttt
     4861 aacatttcca taagttacgc ttatttaaag cgtcgtgaat ttaatgacgt aaattcctgc
     4921 tatttattcg tttgctgaag cgatttcgca gcatttgacg tcaccgcttt tacgtggctt
     4981 tataaaagac gacgaaaagc aaagcccgag catattcgcg ccaatgcgac gtgaaggata
     5041 cagggctatc aaacgataag atggggtgtc tggggtaata tgaacgaaca atattccgca
     5101 ttgcgtagta atgtcagtat gctcggcaaa gtgctgggag aaaccatcaa ggatgcgttg
     5161 ggagaacaca ttcttgaacg cgtagaaact atccgtaagt tgtccaaatc ttcacgcgct
     5221 ggcaatgatg ctaaccgcca ggagttactc accaccttac aaaatttgtc gaacgacgag
     5281 ctgctgcccg ttgcgcgtgc gtttagtcag ttcctgaacc tggccaacac cgccgagcaa
     5341 taccacagca tttcgccgaa aggcgaagct gccagcaacc cggaagtgat cgcccgcacc
     5401 ctgcgtaaac tgaaaaacca gccggaactg agcgaagaca ccatcaaaaa agcagtggaa
     5461 tcgctgtcgc tggaactggt cctcacggct caccctaccg aaattacccg tcgtacactg
     5521 atccacaaaa tggtggaagt gaacgcctgc ttaaaacagc tcgataacaa agatatcgct
     5581 gactacgaac acaaccagct gatgcgtcgc ctgcgccagt taatcgccca gtcatggcat
     5641 accgatgaaa tccgtaagct gcgtccaagc ccggtagatg aagccaaatg gggctttgcc
     5701 gtagtggaaa acagcctgtg gcaaggcgta ccaaattacc tgcgcgaact gaacgaacaa
     5761 ctggaagaga acctcggcta caaactgccc gtcgaatttg ttccggtccg ttttacttcg
     5821 tggatgggcg gcgaccgcga cggcaacccg aacgtcactg ccgatatcac ccgccacgtc
     5881 ctgctactca gccgctggaa agccaccgat ttgttcctga aagatattca ggtgctggtt
     5941 tctgaactgt cgatggttga agcgacccct gaactgctgg cgctggttgg cgaagaaggt
     6001 gccgcagaac cgtatcgcta tctgatgaaa aacctgcgtt ctcgcctgat ggcgacacag
     6061 gcatggctgg aagcgcgcct gaaaggcgaa gaactgccaa aaccagaagg gctgctgaca
     6121 caaaacgaag aactgtggga accgctctac gcttgctacc agtcacttca ggcgtgtggc
     6181 atgggtatta tcgccaacgg cgatctgctc gacaccctgc gccgcgtgaa atgtttcggc
     6241 gtaccgctgg tccgtattga tatccgtcag gagagcaccc gccataccga agcgctgggc
     6301 gagctgaccc gctacctcgg tatcggcgac tacgaaagct ggtcagaggc cgacaaacag
     6361 gcgttcctga tccgcgaact gaactccaaa cgtccgcttc tgccgcgcaa ctggcaacca
     6421 agcgccgaaa cgcgcgaagt gctcgatacc tgccaggtga ttgccgaagc gccgcaaggc
     6481 tccattgccg cctacgtgat ctcgatggcg aaaacgccgt ccgacgtact ggctgtccac
     6541 ctgctgctga aagaagcggg tatcgggttt gcgatgccgg ttgctccgct gtttgaaacc
     6601 ctcgatgatc tgaacaacgc caacgatgtc atgacccagc tgctcaatat tgactggtat
     6661 cgtggcctga ttcagggcaa acagatggtg atgattggct attccgactc agcaaaagat
     6721 gcgggagtga tggcagcttc ctgggcgcaa tatcaggcac aggatgcatt aatcaaaacc
     6781 tgcgaaaaag cgggtattga gctgacgttg ttccacggtc gcggcggttc cattggtcgc
     6841 ggcggcgcac ctgctcatgc ggcgctgctg tcacaaccgc caggaagcct gaaaggtggc
     6901 ctgcgcgtga ctgaacaggg cgagatgatc cgctttaaat atggtctgcc agaaatcacc
     6961 gtcagcagcc tgtcgctgta caccggggcg attctggaag ccaacctgct gccaccacct
     7021 gagccgaaag agagctggcg tcgcattatg gatgaactgt cagtcatctc ctgcgatctc
     7081 taccgtggct acgtacgtga aaacaaagat tttgtgcctt acttccgctc cgctacgccg
     7141 gaacaagaat tgggcaaact gccgttgggt tcacgtccgg cgaaacgtcg cccaaccggc
     7201 ggcgtcgagt cgctgcgcgc cataccgtgg atttttgcct ggacgcaaaa ccgcctgatg
     7261 ctccccgcct ggctgggtgc aggtacggcg ctgcaaaaag tggtcgaaga tggcaaacag
     7321 agcgagctgg aagccatgtg ccgcgattgg ccattcttct cgacgcgtct cggcatgctg
     7381 gagatggtct tcgccaaagc agacctgtgg ctggcggaat actatgacca acgcctggta
     7441 gacaaagcac tgtggccgtt aggtaaagag ttacgcaacc tgcaagaaga agacatcaaa
     7501 gtggtgctgg cgattgccaa cgattcccat ctaatggccg atctgccgtg gattgcagag
     7561 tctattcagc tacggaatat ttacaccgac ccgctgaacg tattgcaggc cgagttactg
     7621 caccgctccc gccaggcaga aaaagaaggc caggaaccgg atcctcgcgt cgaacaagcg
     7681 ttaatggtca ctattgccgg gattgcggca ggtatgcgta ataccggcta atcttcctct
     7741 tctgcaaacc ctcgtgcttt tgcgcgaggg ttttctgaaa tacttctgtt ctaacaccct
     7801 cgttttcaat atatttctgt ctgcatttta ttcaaattct gaatatacct tcagatatcc
     7861 ttaaggaatt gtcgttacat tcggcgatat ttttttcaag acaggttctt actatgcatt
     7921 ccacagaagt ccaggctaaa ccccttttta gctggaaagc cctgggttgg gcactgctct
     7981 acttttggtt tttctctact ctgctacagg ccattattta catcagtggt tatagtggca
     8041 ccaacggcat tcgcgactcg ctgttattca gctcgctgtg gttgatcccg gtattcctct
     8101 ttccgaagcg gattaaaatt attgccgcag taatcggcgt ggtgctatgg gcggcctctc
     8161 tggcggcgct atgctactac gtcatctacg ggcaggagtt ctcgcagagc gttctgtttg
     8221 tgatgttcga aaccaacacc aacgaagcca gcgagtattt aagccagtat ttcagcctga
     8281 aaattgtgct tatcgcgctg gcctatacgg cggtggcagt tctgctgtgg acacgcctgc
     8341 gcccggtcta tattccaaag ccgtggcgtt atgttgtctc ttttgccctg ctttatggct
     8401 tgattctgca tccgatcgcc atgaatacgt ttatcaaaaa caagccgttt gagaaaacgt
     8461 tggataacct ggcctcgcgt atggagcctg ccgcaccgtg gcaattcctg accggctatt
     8521 atcagtatcg tcagcaacta aactcgctaa caaagttact gaatgaaaat aatgccttgc
     8581 cgccactggc taatttcaaa gatgaatcgg gtaacgaacc gcgcacttta gtgctggtga
     8641 ttggcgagtc gacccagcgc ggacgcatga gtctgtacgg ttatccgcgt gaaaccacgc
     8701 cggagctgga tgcgctgcat aaaaccgatc cgaatctgac cgtgtttaat aacgtagtta
     8761 cgtctcgtcc gtacaccatt gaaatcctgc aacaggcgct gacctttgcc aatgaaaaga
     8821 acccggatct gtatctgacg cagccgtcgc tgatgaacat gatgaaacag gcaggttata
     8881 aaaccttctg gatcaccaac cagcagacga tgaccgcccg caataccatg ctgacggtat
     8941 tttcgaagca gaccgacaaa cagttctata tgaaccagca acgtacgcag agtgcgcgtg
     9001 aatacgacac caacgtgctg aaaccattcc aggaagtgct gaaggacccg gcgccgaaga
     9061 aactgatcat cgttcatctg ctgggtacgc atatcaaata caaataccgc tacccggaaa
     9121 atcagggcaa gtttgatggc aataccgatc atgttccgcc tggattaaac gcggaagagc
     9181 tggagtcata taacgattat gacaacgcta acctgtataa cgatcatgtg gttgccagcc
     9241 tgattaaaga ctttaaagca gcaaacccga acggtttcct ggtttatttc tctgaccacg
     9301 gtgaagaggt ttacgacacg ccgccgcaca aaactcaggg gcgtaacgaa gacaacccga
     9361 cgcgccacat gtacaccatt ccgttcctgc tgtggacgtc ggaaaaatgg caagcgactc
     9421 atccgcgtga tttctcgcag gatgttgatc gtaaatacag cctggcggaa ctgatccaca
     9481 cctggtcaga tttagcaggc ttatcttacg acggttacga tccaacccgt tcagtggtga
     9541 atccgcagtt caaagaaact acccgctgga ttggtaaccc gtacaagaaa aacgcgctga
     9601 tcgattacga cacgctgccg tatggcgatc aggtgggtaa tcagtaaatg acctttgtat
     9661 cgcgtctggc gctatttgcc ggatgcgata ctgccgcaat gtttacatcg aactcccctc
     9721 aggccacact tttcaggtgg ccttttttaa taatgccagt tactgttttc ccgtctacat
     9781 acctaaggga ataacatgta tcacgacgtc agctatctgc tctcccgcct gatcaacggc
     9841 ccgctgtccc tgcgccagat ttactttgcc agcagtaacg gcccggtgcc cgatctcgcg
     9901 tatcaggtcg attttccacg gctggaaatt gtgctggaag gtgagtttgt tgataccggc
     9961 gctggagcaa cgttagttcc cggcgatgtg ctgtacgttc ctgctggtgg ctggaatttt
    10021 ccacaatggc aagcccccgc tactaccttt agcgtgctgt ttggcaaaca gcaactcggc
    10081 ttcagcgtcg tgcaatggga tggcaaacaa tatcaaaatc tggcgaagca acacgtcgcc
    10141 cggcgcggcc cacgcatagg ttcttttctg ctacaaacgc tcaatgaaat gcaaatgcag
    10201 ccgcaggagc agcaaacggc aaggcttatc gtcgccagcc tgcttagcca ctgccgcgat
    10261 ttgctcggca gccaaatcca gaccgcctca cgcagccagg cactatttga agctattcgc
    10321 gattatatcg acgaacgcta tgcctccgcg cttacccgcg aatctgtcgc acaggcattc
    10381 tatatttcac caaactatct ctcgcacctg tttcaaaaaa cgggggccat tggttttaac
    10441 gaatacctga atcacacgcg actggagcac gctaagacgt tattgaaagg ctatgacctg
    10501 aaggtaaaag aagtggcgca cgcctgcggc tttgtcgaca gcaactactt ctgccgtctg
    10561 tttcgtaaaa acaccgaacg ctcgccgtcg gaatatcgtc ggcagtacca cagccaactg
    10621 acagaaaaac cgactactcc agaataagat gggtttgctg cggcgcagaa agcactttgc
    10681 gcaccgcgct cattacccgc tgcggctcac gcaggaatgc gtagatgctg cattgcacat
    10741 agcggcaatg ttcaaatcgc tcggcaccgg caagctcgat atccgtaatc aacagagcaa
    10801 catccgcccg acggatatcc tcgtccgcta aacgattctc cgttcccagc gcgccctgag
    10861 tttcaatgct gacgccccac ttctctaact ggcacaactt ttccagccgt tccgccgcca
    10921 tataagtatg cgccacgccg cttacgcagg cggttactgc caccaggtat gccattttaa
    10981 cctcccacgg taacctgaaa tccggcccgt tctgccattt cgcgcatcgt tgtcacatcg
    11041 gctgacgacg gcgcaggcac ctctttcatc gaccatgttt tccccagcag gcggtatttc
    11101 ggttcgccgt actgatgaaa cggtaacaga tggatctgcc tgattttcag cgggatcagt
    11161 acatccagcg cctgctgcat attctcccgg ctgagcgtga aaccagggat caacggtaaa
    11221 cgcgggatca cgttgacacc ctcactcacc agcaaacgca gattctccag cacgcgtggc
    11281 aggttcatct tcaccacatc ccgcgcctga gtcgcgtcca taatttttaa atcgaacagc
    11341 acttcatcgc acaatttcgc cagtggtaac agcttgctgg ctggcgcgtc tccggcagtt
    11401 tcaatggcgc atgacacgcc ccacaaccgc agccgctgta aaaaacgggt agcaaactcc
    11461 gcctgcatta acacttcgcc gccagaaagc gtgacgccgc cgccggacgt gcgaaagaaa
    11521 atgtcatctt tcatcacttc ccgttccagc gcgtcaaggc tgatatcgcg accaatccgt
    11581 tcaaacgccc cggaggggca ttcatccgca tcacgcaaac atttcgcaca gtgcagacat
    11641 ttcgcctctc tgcgtaccgt ctggattttg ccggagatcg actccggatt agcacaccat
    11701 gggcaaagat gcggacagcc tttaaaaaag accaccgtac gaatgccctc gccgtcattc
    11761 agtgaataac gctgaatgtt gaaaatgcgc gccacatcat tgcggcgcgt ttccacaacg
    11821 ttacagctga tgcgctgtcc ggcggatgat gtcatcctgg atctccttcg acagttcgac
    11881 aaagaaggcg ctgtatccgg caacgcgcac caccagcccg gcataatctt gtgggcgctg
    11941 ttgcgcttcc cgcaacgtgt cggcgttcac cacgttaaac tgaatatgct gcaacttaag
    12001 ctgggtaaac gcccgtaaga agtcggccag tttgcgtaat cccgcttcac cttccagggt
    12061 cgccggagtg aatttcacgt tcagcaacgt accgttagac agcagcgtat tatcgagctt
    12121 actgactgac ttcagtaccg ctgttggccc ttgtgcgtcc tggcccagca taggtgacaa
    12181 gccgccgtct gccagttgtt ccccggcaaa acgaccgtct ggcgtcgcgc caaccaccga
    12241 tcccaacggg acgtgagcag aaacggtata cgatcccggc gtgaagtaac caccgcgcgg
    12301 gttctggtat ttttccactt ctttgcagta gtggcgtaac agttcggcgc tgatgttatc
    12361 cacctcgtcg atatcgttac cgtatttctc gaagcggtta attaagcgag cacggacttt
    12421 ttcgccttct ggcgttgcaa agttggcttt taataccgac agcaattcgt caaaacttaa
    12481 acgctgttgc tcaaaaacca tccctttcaa cgcatggaga gaatcgctca ggttggcgat
    12541 accgatcccc tgtacgccgg agaagttata acgcgcgccg ccatcggtaa tatcgcgacc
    12601 tttttccaga caatcgctga taaaagacga gagcagcggt acaggtgccc agtcgcgatg
    12661 gccgatatca caaatattgc tgccctcaac catcagggtg atgtagtggc tgatcttggc
    12721 gcggatctgc tccagtaaac cttcataagt cagcgcagca ttgccttcat tttcatgcag
    12781 gcagatttcc atcactttca gcagattaaa catcgcgatg tcatgcaagc cgtaggttct
    12841 gccgggaata gataattcca cacagcccac tacggaatag tcgcgcgcat cttccagcga
    12901 aacgccacgg ttgaggaacg ctggtaccac cacttcatcg ttaaagattt gcggaatacc
    12961 ggtgccgagg cgaatggttt cggcggtttt catcaggaac ggcgtgtcga tcaaggcgtt
    13021 agtgcgcacg ccgaggttcg gttgcggtaa ttgcacgctt tgataagcgt caaggcaaag
    13081 gaacgaaagc acgttcaccg cgctacgtcc gttctcggtt aacccgccga gcagtgcggt
    13141 atagccggtc gggaaacctg cgaaataacg cgcgctgctg gtggagcgca acagcacgat
    13201 gtcgttgcat ttcacccata aagattcgag tagttctttc aggaacgccg gatcttcgcc
    13261 ctgggttaaa gatgcctgat agaacggcaa catatactgg tcgaagcgcc ccaacgatag
    13321 cgaactggcg ttggattcgt attgcagaat gatgttcatg taccagaata actggcacgc
    13381 ctgccagaac gtctgcggct tatgttcggc gttatggcgg gagatctccg caatagtcag
    13441 cagttcttcg cgacgctggg catctgtgca gtttgccgcc attgtttccg ccagttcagc
    13501 gtaacgcaga atgtgtttct gcgaggcttc cagcagtaac agcgcggcct gataaaagtg
    13561 attctccggc tgttgctgac aatgttgctg catctgcgcc accagctccc ccagcccgtg
    13621 attcagcagg cgtgggtaat caataataat gtgcccctgg cctttatctg tctggttgat
    13681 gctgaaaatc tgcgtgctgg tcgcggcttt tacttcatct gtcatctgcc cgttgatgaa
    13741 atctttcatc gaacgttttt cccagtacgg gaacaactct tcacggtaga tacgtttatc
    13801 ttcttcactg atagcaaagc ggtcctgcgg acgcgtcggg aattgatcca gctctttcag
    13861 cagccagtaa gggtccattt ccggcgacat aatcccagcg cgcggtttta cggtgcggtt
    13921 accggcaatc agttcttcat cacgaatcga aatttcaaca tgttcaagga tatacgctgt
    13981 tgctttcgcc cggcgcaata tcaccggttc gccttcggtt tgccgatggc tggcggtata
    14041 aagcagcgcc cgctccagcg agatttcacg ggtattggca aacagtgcag ttttgaggcg
    14101 agagatacga ttcgtcatga ggaactcctg tgggaaagcg aacccgccct tgcgagcggg
    14161 agaagaactt aagcggtttg cgccaggtga gcttcaattt tgctcataat ggcgtcagca
    14221 cgttttactg catcgctgat gttgacgcgc acgatggtct tgccagcaaa acgctcttca
    14281 aacttgatgc cgatatcttt ggtcagaatc accatatcag cgttcgccac atcttccgca
    14341 gtcagttcat tttccagacc aattgacccc tgggtttcta ctttcacttc ccagcctttc
    14401 gctttcgcgg cactttccag cgcctctgct gccatatagg tatgggcaac acctgaagga
    14461 catgcggtta ctgcaataat tttcgtcatg gctcgttcct caattaatta atttcaaaat
    14521 ccaaatccag gtcgtcttct ttttcatcag tcgaagaccc atttttacgc gccagacttt
    14581 tcagcacgtt cacacaaaca gccgtcacca ctgctccgac agccactgct gcgatataac
    14641 ccagcttgcc ttcaaccacc ggcagcacaa tcagaccacc ccagcctgca tagcactgcg
    14701 caccgaccag cgccgcagtt actgcaccac aaactgaacc gaccatgatg gaaggaatga
    14761 cacgcagcgg atcggcagcg gcgaaaggaa tcgccccttc agtaacgcca acacagccca
    14821 tcaccagtgc cgctttgccg gtttcgcgct cttctgcgga gaaattttta cgactaatca
    14881 gcgtcgccag ccccatcccc agcggcggga tacaaatacc cacggcagcg atggccacca
    14941 cggtataaac accctgagca acgcaaatca gcatgaaggc ataggccact ttgttaaccg
    15001 gaccgcccat atcgaacgcc agcatcagac ccatgatcac cgccagcata acaatgctgc
    15061 cctgctgcat cccctgaagc cactgagtca ggctgttggt caacgcccct acaggctcgc
    15121 ccaagcccca catcatgatg cctgcggtaa tcagtgtgcc gacgatagga ataatgaaga
    15181 taggcatcac cgagcgcaga actttatgca ccggaatttt cttcaggtaa tgcaccacga
    15241 tgccgccgat aatcccggcg atcagtgcac caaagaagcc cgcaccaaag ctgttaccaa
    15301 cccaggcacc gatagcgcac ggagccagcg cagaacgctc tgcaatggaa taaccgatgt
    15361 acgctgcgag gaaaggcacc atcagcgtca agcccgcaac gccgatatca aacagttttt
    15421 tcagatttgg atcggctacg gcatccggca ctgcgccttt gccatacaac atgacggaaa
    15481 ccgccagcaa aataccgccc gataccacga agggaatcat gtgtgaaacg cccgtcatta
    15541 aatgctgacg ggtatttttc aggatctgca ccaactcatt cataagctgc tcccgggctt
    15601 gcgaaagccc atgtccatat ttttgatgga ggaaacaata ggcaatcgca ggcggagcaa
    15661 acagtggata aaaaggccat ttactggact tttgtaatat cagtacaaaa atgcgatccg
    15721 cctcataact tgcgataaac cggaagcatg ccgcaaaaca gcgtcttgtg agacgcgtcg
    15781 caaattgacg gttacattac atttgtccag cttttggcag atttgtcaca tggcaggccg
    15841 ttgccgtgcg ctcttatcct gtaaacagaa aaccgtaccg ggagagaggc aatggccctg
    15901 attgtggaat ttatttgtga gctacctaac ggcgtacatg cacgtccggc aagccacgtt
    15961 gaaacgctgt gtaatacttt ttcatcacaa attgagtggc ataacctgcg cactgaccgc
    16021 aagggcaacg ccaaaagcgc ccttgcgctg attggcaccg atacgctggc gggcgataac
    16081 tgccagttac tgatttccgg ggccgacgaa caggaagcgc accagcgttt aagccaatgg
    16141 ctgcgcgatg aattccccca ctgcgatgcg ccgctggcgg aagttaaatc tgacgaactg
    16201 gaaccactgc cggtttcact gaccaatctg aatccgcaaa ttatccgcgc ccgcaccgtg
    16261 tgcagcggta gtgcaggcgg cattctgacg ccgatctctt ctttagatct caatgcgctg
    16321 ggtaatcttc ccgcagccaa aggcgttgac gccgagcaat ccgcactgga aaacggcctg
    16381 acgctggtac tgaaaaacat tgagtttcgt ctgctggata gcgacggtgc caccagcgcg
    16441 attctggaag cgcaccgatc cctggctggc gatacttccc tgcgcgaaca tttactggca
    16501 ggcgtcagcg ccggattaag ctgcgccgaa gcgattgtca ccagtgcgaa tcacttttgc
    16561 gaagagttcg cccgttccag cagcagctac ctgcaagaac gtgccctgga cgtacgcgac
    16621 gtctgcttcc agttactcca gcaaatctac ggtgagcaac gcttcccggc accgggcaaa
    16681 ctgacgcagc ccgccatttg tatggctgat gaactgactc ccagccagtt cctcgaactg
    16741 gataaaaatc acctcaaagg tctgttactg aaaagtggcg gcaccacctc acataccgta
    16801 attcttgccc gttcgttcaa cattccgaca ctggttggtg tagatattga tgcccttact
    16861 ccgtggcagc atcaaacgat ttatatcgac ggcaacgctg gggcgattgt ggttgagcca
    16921 ggagaagccg tagctcgtta ttatcagcaa gaagcccgcg tacaggacgc cctgcgtgag
    16981 caacagcgtg tctggctgac ccaacaagcc cgtaccgctg acggtatccg cattgaaatt
    17041 gccgctaaca tcgctcactc cgtggaagcg caggccgcat tcggcaatgg tgcggaaggc
    17101 gttggtttgt tccgcactga aatgctctat atggatcgca ccagtgcacc gggcgaaagc
    17161 gagctgtaca acattttttg tcaggcgctg gagtctgcca acggacgcag cattattgtg
    17221 cgcactatgg acattggcgg cgacaaaccc gttgattatc tgaacattcc ggcagaagct
    17281 aacccgttcc tcggttatcg cgctgtgcgt atttatgaag aatacgcctc gctgttcacc
    17341 acacaactac ggtcgatcct gcgcgcctcc gctcacggca gcctgaaaat catgatcccg
    17401 atgatctcct caatggaaga gatcttatgg gtgaaagaaa aactggcgga agccaaacag
    17461 caactgcgta acgaacacat tccgtttgat gagaagatcc agcttggcat tatgctggaa
    17521 gtaccgtcgg tgatgttcat catcgatcaa tgctgcgaag agattgattt ctttagtatt
    17581 ggtagtaatg acctgacgca atatctgctg gcagtggatc gcgataacgc taaggttact
    17641 cgtcactaca acagcctgaa tccggcattc ttgcgggcgc tcgattacgc cgtgcaggcg
    17701 gtgcatcgcc agggcaaatg gattggtctg tgcggtgagc tgggagcgaa aggttccgtg
    17761 ctgccgttgc tggtcggctt agggctggat gaactcagca tgagcgcacc atcaattccg
    17821 gcggcgaaag cgcggatggc gcaacttgat agccgtgagt gccgcaagtt gctcaaccag
    17881 gcaatggcct gccgtacttc gctggaagtg gaacacctgc tggcgcaatt ccgcatgacc
    17941 caacaggacg caccgctggt caccgccgag tgcatcacgc tggaaagtga ctggcgcagc
    18001 aaagaagaag tgctcaaagg catgaccgat aacctgctgc tggcgggccg ctgccgctat
    18061 ccgcgtaaac tggaagccga cttatgggcg cgcgaggccg ttttctctac cggtctgggc
    18121 tttagttttg ccattccaca cagcaaatca gaacacattg agcaatccac catcagcgtg
    18181 gcgcgtctgc aagcgccggt gcgctggggc gatgataaag cgcaattcat cattatgtta
    18241 accctgaaca aacacgctgc gggcgatcag cacatgcgca ttttctcgcg cctcgctcgc
    18301 cgcatcatgc acgaagaatt ccgtaacgcg ctggttaacg ccgcctctgc cgacgctatc
    18361 gccagcctgc tgcaacatga actggaactg taaaaggaaa catcatggaa ctgtatctgg
    18421 acaccgctaa cgtcgcagaa gtcgaacgtc tggcacgcat attccccatt gccggggtga
    18481 caactaaccc gagcattatc gctgccagca aggagtccat atgggaagtt ctgccgcgtc
    18541 tgcaaaaagc gattggtgat gagggcattc tgtttgctca gactatgagt cgtgacgcgc
    18601 agggtatggt ggaagaagcg aagcgcctgc gcgacgccat tccgggcatt gtggtgaaaa
    18661 tcccggtgac ttccgaaggt ctggccgcca ttaagatgct gaaaaaagag ggtattacta
    18721 cgcttggcac cgcagtgtac agtgctgcac aagggttact ggccgcgctg gcaggggcaa
    18781 aatacgttgc tccgtatgtt aaccgcgtag atgcccaggg cggggacggc attcgtacgg
    18841 ttcaggagct gcaagcgctg ttagaaatgc acgcaccaga aagcatggtg ctggcggcca
    18901 gctttaaaac gccgcgtcag gcgctggact gtttactggc aggatgtgaa tccatcaccc
    18961 tgcccttaga tgtagcgcaa caaatgctca acacccctgc ggtagagtca gctatagaga
    19021 agttcgaaca cgactggaat gccgcatttg gcactactca tctctaaagg agcaattatg
    19081 gaccgcatta ttcaatcacc gggtaaatac atccagggcg ctgatgtgat taatcgtctg
    19141 ggcgaatacc tgaagccgct ggcagaacgc tggttagtgg tgggtgacaa atttgtttta
    19201 ggttttgctc aatccactgt cgagaaaagc tttaaagatg ctggactggt agtagaaatt
    19261 gcgccgtttg gcggtgaatg ttcgcaaaat gagatcgacc gtctgcgtgg catcgcggag
    19321 actgcgcagt gtggcgcaat tctcggtatc ggtggcggaa aaaccctcga tactgccaaa
    19381 gcactggcac atttcatggg tgttccggta gcgatcgcac cgactatcgc ctctaccgat
    19441 gcaccgtgca gcgcattgtc tgttatctac accgatgagg gtgagtttga ccgctatctg
    19501 ctgttgccaa ataacccgaa tatggtcatt gtcgacacca aaatcgtcgc gggcgcacct
    19561 gcacgtctgt tagcagcggg tatcggcgat gcgctggcaa cctggtttga agcgcgtgct
    19621 tgctctcgta gcggcgcgac caccatggcg ggcggcaagt gcacccaggc ggcgctggca
    19681 ctggctgaac tgtgctacaa caccctgctg gaagaaggcg aaaaagcgat gcttgccgcc
    19741 gaacagcatg tagtaacccc ggcgctggag cgcgtgattg aagcgaacac ctatttgagc
    19801 ggcgttggtt ttgaaagcgg cgggctggcg gcggctcacg cggtgcataa cggcctgacc
    19861 gctatcccgg acgcgcatca ctattatcac ggtgaaaaag tggcatttgg tacgctgacg
    19921 cagctggttc tggaaaacgc accggttgag gaaatcgaaa ccgtagctgc acttagccat
    19981 gcggtaggtt tgccaataac tctcgctcaa ctggatatta aagaagatgt cccggcgaaa
    20041 atgcgaattg tggcagaagc ggcatgtgca gaaggtgaaa ccattcacaa catgcctggc
    20101 ggcgcgacgc cagatcaggt ttacgccgct ctgctggtag ccgaccagta cggtcagcgt
    20161 ttcctgcaag agtgggaata acctactcca aactcccggc ttgtccggga gtttgaacgc
    20221 aaaattgcct gatgcgctac gtttatcaag cctacgaaat ctctgcaata tattgaatat
    20281 gcgtgctttt gtaggccgga taaggcgttc actccgcatc cggcactctg tgccagtaat
    20341 ctgaaactcc cacgttgttc aggagttttt tatccgcttc tggacatttt ctccacagaa
    20401 caatcgttag cccggaaggt cgaatctgtg actcttattt tcacggacga accagatgaa
    20461 agcttcactg gcgctgctca gcctgctaac ggcatttacc agccactcct taaaatctcc
    20521 cgccgttcca ccaaccgtgg tacaaatcca ggccaatacc aaccttgcca tcgcagacgg
    20581 tgccagacag caaatcggca gcacgctatt ttacgatccg gcgtatatgc agcttaccta
    20641 tcctggcggt gatgtcccgc aagaacgcgg ggtatgttcc gatgtggtga tccgcgccct
    20701 gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa gatatggcga agaattttgc
    20761 cgagtacccg caaaaatgga agttaaagcg cccggacagc aacatcgatc accgtcgggt
    20821 gcctaatctg gaaacctggt ttagccgcca caacaaaaca cgccccatca gtaaaaatgc
    20881 cggtgactat caagcgggcg atattgtctc ctggcgatta gataacgggc tggcgcatat
    20941 tggggtggtg tcagatggct tcgcccgcga cggtacgccg ctggtgatac ataacattgg
    21001 cgcaggcgcg caggaagaag atgtactgtt tagctggcgg atggtggggc attatcggta
    21061 ttttgttaaa taagcgatcg ccccacatga agaaaaaatt cagatgcttt ttacactgac
    21121 agcttctttt ttcttacggc tcaccagcgc cagtgccagc acaatcagca caataccgct
    21181 accttcaacc acgccaggat tctcgccgag cagccaccag gaaaagagta cgccgcaaac
    21241 gggaacggcc agtgtgctta agctggcaat actggcaggc aagtttttca atacaaacaa
    21301 ccataagctc cacgccagcg ccgtcgccag aatcgcactg tagaccagcg cccagaacac
    21361 agtgggctgc cagtcaattt cacgttgtgg caccagtaaa gcgaccacac tcatcaccag
    21421 cgccgcatac agcatctgcc aggatgttaa cgacaataaa tccacgcgcg gatgacgggc
    21481 atacagacgt ttagcaacaa tcgcgctcgc cccccaacta acaccggaga gaattgccag
    21541 catggcactt ttcatcgaag agaaatcgag ttgccacggc tgcaacacca aaaataagcc
    21601 gaaagcggca atcagtatcg cgaaatattg cccacgtcgc aggcgttcac cgagaaacaa
    21661 cgcggcgaaa atcaccaccc agaacggcat ggtatagctc aggatcgcca ccttccccgc
    21721 acctccgctg accaacgccc attgtgccag gccaatcatt ccgcaggttt gtaacagggc
    21781 aatagctaag gtgtatttaa acggcgtcgg gcgcattgcg cgaccacgta ataaaaggac
    21841 gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg gtgaagtcga aggcaccgat
    21901 gtaactggtg acttgcttca tgaaaatcca gctataactc cagataagtg tgagcacaac
    21961 caggccactg attgccagtg ggttgctctt tcctgcggca gacatgagag atccagtgtg
    22021 tagttttcct gtggacacta tacgccggta aagaaacttc agcgactgcc tgaaagacag
    22081 tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg aggttcatca ctttcaccca
    22141 tgccgccacg aagtctttaa caaacttctc gtgggcatcg ctgctggcgt aaacttccgc
    22201 taccgcacgc aggacagagt tagaaccaaa taccagatcc gcacggctgg ctgtgtattt
    22261 cacttcgccg gtttcacggt cacgaccttc gaacagctct ttcgattcgt cagtcgcttt
    22321 ccactcgtaa cgcatatcca gcaagttcac gaagaagtca ttgctcaata cgccaacgcg
    22381 gtcagtgaag acgccgtttt tgctgccatc gaagttggca cccagtacac gcatcccgcc
    22441 caccagcgca gtcatttccg gcgcggtcag cgtcagttgc tgtgctttgt cgatcagcag
    22501 tgactcggtg gtggaaacgt ccagacgagc gcgatagtta cggaaaccgt cagcaattgg
    22561 ctccagcagc tcaaacatct caatgtcagt ctgatcctga cgcgcatcaa cgcgacccgg
    22621 cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg gctttctcaa caccaaccac
    22681 accagccagc acgatgatat ccgccagcga ggctttacca gactctttct ggattttctc
    22741 cagaacaggc agagcacgaa cggctgcggc gttcacatcc cagtcgcgct gcggcattaa
    22801 tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca ccacggaagg tagaagcaga
    22861 tgcccaggcc accgatacca gctcactaac agacagacca gaatccgcaa tcgcgaattt
    22921 cagatcgata atgtcctgct cggtcgggtt gtagatcggc tgcggcagcg gatcttgcca
    22981 gatcagatct tctttcggta cttccggtcc gatgtaacga gatttcggcc ccatatccct
    23041 gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg aacgcctgcg gatcgttgag
    23101 gaaacgacga gagatcttct cgaactcagg atcaaaacgc agcgtcaggt cggtcaccag
    23161 catcgtcggt ttacgtttct tcgacggatc gaacggatcc gggataattt ccggtgcgtc
    23221 taccgcttcg aactggattg cgccagccgg actgcgggtc tgtacccact catatttgaa
    23281 caggttctcg aagaaatagt tgctccactg ggtcggcgtc tgggtccaga caacttccag
    23341 accagaggta atggcatctg cgccaacgcc gctgccgtaa gtgctcgccc agcctaaacc
    23401 ttgttcttca atcggtgcag cttctggatc aggacctaca tttgatgtcg gaccggcacc
    23461 gtgggtttta cccagcgtat gaccacccgc aatcagcgcc acggtttctt cgtcgttcat
    23521 gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca gaaagcggtt cgccgctgtg
    23581 gtccgggcct tccgggttaa cgtaaatcag gcccatctcg gttgcaccca gcggtgcttt
    23641 cgccagcgct tccggatgac ggtgagtcag ccaggctttt tcatcacccc agttaacatc
    23701 cagatccggt tcccagacgt cttcacgacc ggcaccaaaa ccgaaggtac ggaagccgga
    23761 gttttccagc gccacgttac ctgcgaggat aaacaggtcg gcccaggaga ttttctgacc
    23821 atatttctgt ttgattggcc acaacaggcg acgcgcttta tcgaggctta cgttatccgg
    23881 ccaggagttc agcggtgcaa aacgttgctg accacgaccc gcgccgccgc gtccatcgat
    23941 tgaacggtaa gtacccgcgc cgtgccaggc catacgaata aacagaccgg cgtaactgcc
    24001 ccagtcggca ggccaccacg gctgagattc tgtcagcagg gctttcagat cttttttcag
    24061 gccgtagtaa tctaatttgc tgaattcttt gcggtagtca aagtcctcac ccagtgggtt
    24121 agaacgatta gaatgttggt ttaacaggtc aacacggagt tgatttggcc accagtcgcg
    24181 agtggttgtg cccgcccccg cgctctggtc gtgaccgccc tgatggaacg ggcatttgcc
    24241 agtggctgtg gtgttatgaa tatcgtctga cgtgctcatc aatgtgctcc cctctacagt
    24301 gttaccgtta cgatacacag cgttagagag aagttataat tgaattaatc cacggatgcg
    24361 atagttgaga tcttacatat tgttggttaa agagatgtag atcaaattga tcgtaattaa
    24421 gcttttttga ttatgtccga attcggacaa atgctttata aaaaagggtt ttaggttcat
    24481 atccttcaag taatgaggga gaaaacaagg ctgataacct tattttcccg ccctcatttc
    24541 gaggcagcat tttgtgctct gtttaaaatt tgtgatcact gtgtgatttt cacaaaagcc
    24601 acactattta taaaccaggt cgaacaccca gcgtatggca aatcgcgtaa ctcatttcag
    24661 cgcggttaag cgtatagaag tggaaatctt tcactccttc acggcttaaa atcttcacca
    24721 tatccatggc aatattcgct cccaccagtt tgcgggtttc agcatcgtca tccagcccgt
    24781 caaacatttg tgccatccac gccggaatac gcacgttggt catatcggca aatttcttcg
    24841 cctgtttaaa gttagatacc ggcaaaatcc ccggaataat ttccacatca atgcccgccg
    24901 atacgcagcg gtcacgaaaa cgcaggtagc tttcgacatc aaagaagaac tgagtaatcg
    24961 cgcggttggc tccggcatcg actttgcgtt tcaggttgag caaatccgcc tgagcgcttt
    25021 ttgcttccgg gtgaacttcc ggatacgccg ccacggagat atcgaaatct gccacttctt
    25081 ttaacagcgt caccaggtca gaagcataca tttccggctt accacttccc ggcggcagat
    25141 cgccacgcag cgccacgata tgacgaatac cgttattcca gtagtcgcgt gcaatggtgc
    25201 gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg tgccgcttcc agaccagtgc
    25261 gatctttaat gcctttaata atgctgtgcg tacggtcgcg ctcgccggag ttcgcgccat
    25321 aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg atcgatggag ttccacaggg
    25381 tctgctccat ttcactggta cgcggcggga aaaactcgaa cgaaacgtta atctgcccct
    25441 ggacttctgc caggctctga ttcagggcat cccgctggct ggcgtgaaaa aagctcatac
    25501 cttacctcat caatcgcttg tagttatatg ttgtgttgtg cacatctatc catttagacg
    25561 tccagatgta aagatgaagg aaaagccgaa gggcgtcaac cgaaaaatga ccaatgatgg
    25621 tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt aagaaaaaga aaatccggta
    25681 tgcaaagcaa accggactgc aaatataaac taaccatgta cttattgctt cttgccggat
    25741 tcgtcgtaaa cgcttcatcc ggcctaatcc tatgataatt caatatattt cattagaaac
    25801 catagacccg aaaacatgag taccgggcat tattaaattt ctgaaattac aacaactgtg
    25861 ccagccggtt gatatccgac tgaatcgccc cggcggtgac gtcgcgccca gcgccaggcc
    25921 cacggatcac cagcggatta tcgcggtacc agcggctttc gatggcaaag acgttatcgc
    25981 atggcagcag tgatgccagc ggatggtctt cacgcaccgc ttccacgccc acacgcgctt
    26041 tgccattggc atcgaaacgc gccacgtagc gcagcaccag ccccatttcg cgggccgctt
    26101 ccagccgttg caccatctgc tcgttaagtt catcgccatt ttcaaagaaa tggtcgatgc
    26161 tgccgccttc gcaatgagca ggcaccagcg attccacacg tacctggtcc ggttcaatgt
    26221 tgtaacccgc ttcacgcgcc agaattacca gcttgcgcat tacgtctttg ccggagaggt
    26281 cgtcacgcgg atcaggctcg gttaagccct gctgccacgc ctgatccacc agctcggtaa
    26341 acggcacgct accgtcgaat tgcaggaaca gccaggagag cgtgccggag aagatcccgc
    26401 tgatcgacaa aatagtatcg ccgctgtcga tcagatcgcg cacggtgtgg ttgatcggca
    26461 agcccgcacc gacggtggca ttgtacagcc agtgacgccc ggttttttcg aaggcgtcgt
    26521 ggatctggcg atatttattg ctgtcgctgg ctcccgccag tttgttggcg ctgataacgt
    26581 ggaaaccgtg gctggcgaaa tcaagatact gatcagcaag ctgctggctg gcggtaacgt
    26641 ccagcaccac taaatcatca tacggatggg cgcgcatcca caggaacaac gactcttcat
    26701 cctgctcaac cgcttcatcg ttgaagaagg ctaacgcgcg gctggcgtcc agcccgtcat
    26761 agctcaacag gctgcggcgg ctgtccacca cacctgccag cacaaactca aagccggtac
    26821 gtgccgaaag cgtgctctgc tcacgggcga acagttccag ccaacgggaa ccgatattgc
    26881 ccttaccgaa caataccagg ccgatgcgtt tttctgcgcg gaagacggac tgatgcagcc
    26941 cctgaatcag gctttcggtc gggccggtgc gcagtactgc caccaggctg atgccgtcat
    27001 cggactgcca ggtaaattcg accggctggc ctttcagttg ctgccagaag cggtggcaat
    27061 gcagcgggtt acgggtgacg cctgcaccga ccatcgccac cagcgccagc ccctgacgca
    27121 ggcgcagttc gccaggtaat cccgcttcgt cgaggatttt cagcgcactg tcggccactt
    27181 ctgaggtgta gcaaaattgc agcaactggc gatcgttatg tacgccaacc gccagcgggc
    27241 gtacctgcgc gcgtttcagg atttggtcga tctctttatg cgccagtttg aaatcctgac
    27301 tggcgggcac ctgaaactca atcaaacaga catcatcgtg gctggtgaca atacgcgcac
    27361 cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc ttgatccggc gtgtagctac
    27421 agcgcagttg caggtcgatt tcgctgccag aaaccggctg taaagtacgg gcgtgaagaa
    27481 cgggagccgc caggcgcgcc agttcgctgg cctcatccag acgcagcaac ggcagcaggc
    27541 aggcatcttt cactttacgc gggtcggcac tgtatacccc ggcgacgtcg ctccagatgg
    27601 ttacgcgaga aacacccgcc agcgcaccga tttgtgtcgc ggaatagtcg gaaccgttac
    27661 gccccagcag caccgtttca ccggcgttgt tgcggctgat aaatccggtc accaccagac
    27721 gtttgcccgg atgttgcacc agcagctgtt gcagcaacgg gtaagaaagc ccttcatcaa
    27781 cctgcggttg tgcggcgcgt tcagcgcgta aaaactcgcg ggcatcaagc caggccgctg
    27841 gcagcccttg ttgattaagt accgcagaca tcaggcgtgc cgaccacact tcaccgtgcc
    27901 ccaccacttc cgcatacact gcgtcgttaa taccgctgtc gagcagcgcc gccaggcgct
    27961 caaggtcgct gacaaaagcg ctaatgaggc tatcggcttc ttcagcgggt agcagaccgc
    28021 taatcagatc gcactgatag cgacgcagcg tttgttgaac ctgatgcgca gagagacgat
    28081 cggtctggct tagtttcaac cagttaatca actggttagt ggtgctaccg gcggcggaaa
    28141 ccaccatcat atcgtcaggc tgagagtact ccgccataat gcccgcgaca cgcaaataac
    28201 acttcacatc agccagacta ctgccaccaa atttatgcag ctgacgacct ttcgcccctg
    28261 cctgcgcaat cacactcatt tttacccctt gtttgcagcc cggaagccat tttccaggtc
    28321 ggcaattaaa tcttcgccat cttcaatacc ggtggagata cgcagcagcg tctcggagat
    28381 cccggcggca gcacgcgctt ctggtgccat gcctgcatgt gtcatggttg cggcgtgaga
    28441 gattaaactt tccactcccc ctaatgattc cgccagcgta aacaacgaca gcccgctcag
    28501 gaaacgacgc agcgtctgct catcgccatc cagttcaaaa ctcaacattg cgccaaagcc
    28561 tttttgctga cgcgcggcaa tttcatgccc ctgattttcc ggcagcgacg ggtgatacag
    28621 ttttttcacc aacggctggg tttgcagata tttcacaatc gcctgcgcgt tacgctgggc
    28681 caactccatg cgcggcacca gcgttcgcaa cccgcgtagt agcagatagc tgtcaaacgc
    28741 gccgcccgtc acgccaatat tgtttgccca ccaggcaagt tcagtgacaa cgtccgggtc
    28801 tttagcaatc accacgccgg ccaccacgtc tgagtgaccg ttcagatatt tcgtgcatga
    28861 atgcaacacc agatcggcac ctaatgccag cggattttgt aatgccgggc ttaagaaggt
    28921 gttatccacc acgctcaccg ccccgacttc ccttgccaga tggcagattt tcgcaatatc
    28981 cacgacgcgt aacaatggat tacttgggct ttctaccagt accagtttgg gtttttctgc
    29041 cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca aacaacacgc gatagcaacc
    29101 acgtttcgcc aggctgtcga acaggcgata gctaccgccg tagcagtcgt gcggcgctac
    29161 cagcagatcg ccaggtttca aaaagacggt cgttaccagg tgaatcgcgg acatgccggt
    29221 attagtaagt actgcaccag caccgccttc cagttctgcc agtgctcgct gaaccacatc
    29281 gcgcgttggg ttgccacgac gcgagtaatc atgcgcgcgc ggttcattaa atccggtaaa
    29341 gttataggta ctggaaagat ggatcggtgg gacaacgcaa ccatactgtt cgtcgtcatt
    29401 taacccgcta cgcactgcga tggtggcctg tttacgcgtc atgtgatgaa gttccctggg
    29461 cttggtcggt gaaatgtcag gcaccagagt aaacattgtg ttaatggacg tcaatacatc
    29521 tagacatcta aacttctttg cgtatagatt gagcaaatcc caaatagccg ttaaaattat
    29581 atgcattatc acgccgacag gtgcattaca cgatgtcacg gtaacgcctg tacggtaaac
    29641 tatgcgggtt tacggtcagt acccacataa attgtgtggt ctggtctcaa tttattgacg
    29701 aagaggatta agtatctcat ggctgaatgg agcggcgaat atatcagccc atacgctgag
    29761 cacggcaaga agagtgaaca agtcaaaaag attacggttt ccattcctct taaggtgtta
    29821 aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca acctgcgtca cgctaccaac
    29881 agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg ggcaacctct gccggatgat
    29941 gccgatctgc gtaaagagcg cagcgacgaa atcccggaag cggcaaaaga gatcatgcgt
    30001 gagatgggga ttaacccgga gacgtgggaa tactaaagca aaaaagagcg gcgcggagtg
    30061 gaatcgcctg atgcgctacg cttatcaggc ctacgtcata ttgcaattta ttgaatttgc
    30121 acgaacttgt aggccggata aggcgttcac gccgcatccg gcataaacaa cgagcacgtt
    30181 gtctgcgacc caccgctttt tatacatgga cgtttaacta tgaaaaacag gctgctgatc
    30241 ctcagcctgc tggtttctgt acctgccttt gcctggcagc cgcaaaccgg cgacatcatc
    30301 tttcagatct ctcgctcatc gcaaagtaaa gcgatccaac tggcgaccca ttccgattac
    30361 agccacaccg gtatgctggt gatacgcaac aaaaagccct acgtttttga agcggtcggc
    30421 ccggtaaaat acaccccgct caagcagtgg atcgcccatg gtgaaaaggg taagtacgtt
    30481 gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc aaaaactggc gcaaacggca
    30541 aaacgttatc tcggtaaacc gtatgatttc agcttctcat ggagcgacga tcgccagtac
    30601 tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg gaatgcgcgt gggcgagcag
    30661 cagaagttga aagagttcga tctcagcaac ccgctggtcc aggcgaagct caaagaacgc
    30721 tacggcaaaa atattccgct tgaggaaacg gtagtttcac cacaagccgt tttcgacgcg
    30781 ccacaactca ccacggttgc caaagagtgg ccgctgtttt cgtggtaatg gatgatgaaa
    30841 aaaataattc ccgccgtagt ctgtttgttg ctttgtagtt gcacgacctt tccgcaagct
    30901 gtcgctccgg taaacggtga tgcctccaat tagttgaatt aaggatataa tactcgcttt
    30961 taaggttgtt tcatggtcac agttaacctt cattgccccc gttgccagtc cgttcaggtt
    31021 taccgtcacg ggcagaaccc aaaaagacat gatcgattcc gcggccgtga ctgtcaccgg
    31081 gtatttcagc tcagttactg ttacgagccc cgtaaaccgg gagttaaaga ccaaatcgct
    31141 gaaatgactt tcaacggtgc cggggttcgc gatactgccc gtacgctgaa aatcggcatt
    31201 aatacagtca tccgaacttt aaaaaactct cgccacgaag aataacgtca tccccggtcg
    31261 ctcatgctga tgtggcactc atctgcaaac ttagtcagca atggagcttt gtcggcagta
    31321 aagcgcgaca gcactggctc tggtacgtgt acaacacgaa aactggtggt gttctggcct
    31381 acacttttgg cccccgggct gacgaaacct gtcgtgaatt gcgggcgctg cttacccttt
    31441 taccatcggc atgttgacca ctgacgaatg gggtagttac gctagagagt taccgaagga
    31501 gaagcatctg actggtaaga tttttactca gcacattgag cgtaacaatc tgaccctgag
    31561 aacccgaatc aaacgtctgg cccgcagaac catctgtttc tcgcgttctg tagaacttca
    31621 tgaaaaagtc atcggtgctt ttatcgaaaa tatatgttct actaattgga gtcatcaccc
    31681 ttctgataag ctttccttgg atgattacca gactgaatac acagccaaag atttttacct
    31741 cattctgctc ttttttctca aatgacatat attatatttt acatcatatt cataggcata
    31801 tgacattccc c
//
LOCUS       DAJOWB010000045        31015 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJOWB010000045 DAJOWB010000000
VERSION     DAJOWB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.045"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="OHO85_002247"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="OHO85_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCQ2671613.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="OHO85_002248"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="OHO85_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCQ2671614.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="OHO85_002249"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="OHO85_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCQ2671615.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="OHO85_002250"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="OHO85_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCQ2671616.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="OHO85_002251"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="OHO85_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCQ2671617.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="OHO85_002252"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="OHO85_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCQ2671618.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="OHO85_002253"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="OHO85_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCQ2671619.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="OHO85_002254"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="OHO85_002254"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCQ2671620.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="OHO85_002255"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="OHO85_002255"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCQ2671621.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12889..13428
                     /locus_tag="OHO85_002256"
     CDS             12889..13428
                     /locus_tag="OHO85_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HCQ2671622.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="OHO85_002257"
     CDS             complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="OHO85_002257"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCQ2671623.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="OHO85_002258"
     CDS             complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="OHO85_002258"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCQ2671624.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETCAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="OHO85_002259"
     CDS             complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="OHO85_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCQ2671625.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="OHO85_002260"
     CDS             complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="OHO85_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCQ2671626.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="OHO85_002261"
     CDS             complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="OHO85_002261"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCQ2671627.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="OHO85_002262"
     CDS             complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="OHO85_002262"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCQ2671628.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19283..19383)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="OHO85_002263"
     CDS             complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="OHO85_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCQ2671629.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20006..20779
                     /gene="nudC"
                     /locus_tag="OHO85_002264"
     CDS             20006..20779
                     /gene="nudC"
                     /locus_tag="OHO85_002264"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCQ2671630.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20819..21883
                     /gene="hemE"
                     /locus_tag="OHO85_002265"
     CDS             20819..21883
                     /gene="hemE"
                     /locus_tag="OHO85_002265"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCQ2671631.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLVRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21893..22564
                     /gene="nfi"
                     /locus_tag="OHO85_002266"
     CDS             21893..22564
                     /gene="nfi"
                     /locus_tag="OHO85_002266"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCQ2671632.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22607..23197
                     /locus_tag="OHO85_002267"
     CDS             22607..23197
                     /locus_tag="OHO85_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCQ2671633.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23384..23656
                     /gene="hupA"
                     /locus_tag="OHO85_002268"
     CDS             23384..23656
                     /gene="hupA"
                     /locus_tag="OHO85_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCQ2671634.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23669..24364
                     /locus_tag="OHO85_002269"
     CDS             23669..24364
                     /locus_tag="OHO85_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCQ2671635.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="OHO85_002270"
     CDS             complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="OHO85_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCQ2671636.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25029..26405
                     /gene="zraS"
                     /locus_tag="OHO85_002271"
     CDS             25029..26405
                     /gene="zraS"
                     /locus_tag="OHO85_002271"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCQ2671637.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26402..27727
                     /gene="zraR"
                     /locus_tag="OHO85_002272"
     CDS             26402..27727
                     /gene="zraR"
                     /locus_tag="OHO85_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCQ2671638.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27724..29013)
                     /gene="purD"
                     /locus_tag="OHO85_002273"
     CDS             complement(27724..29013)
                     /gene="purD"
                     /locus_tag="OHO85_002273"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCQ2671639.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29025..30614)
                     /gene="purH"
                     /locus_tag="OHO85_002274"
     CDS             complement(29025..30614)
                     /gene="purH"
                     /locus_tag="OHO85_002274"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCQ2671640.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKLDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPSEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7312 a         8043 c         8476 g         7184 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacac cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttagtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gtatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5101 tcttttgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5341 cgtatcgaaa cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacttgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcact acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaagtga ccgacggtgt tgtaactgac gaaattcact acctgtctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgcga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgacagacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtatatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcgggttaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggggcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gtactgggtc tgtactacat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcta acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggctgatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg ttaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaagggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaata ctgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 tcagaccatt actcgtcaga cagacgaatt gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gcacgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtggggg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaaggaat ttctgaatac
    12781 tcataatcaa tgtagagatg actaatatcc tgaaactgac tgaactaatt gagtcaaact
    12841 cggcaaggat tcgatactat tcctgtgtaa ctttcttaag gaacgagaat gaaacaggaa
    12901 gtggaaaagt ggcgaccttt tggacatccg gatggtgata ttcgtgattt atcatttctt
    12961 gatgctcatc aggctgtcta cgttcagcat catgagggca aagagccttt agagtatcgc
    13021 ttttgggtta cctactctct tcactgcttc acaaaagatt atgaacatca gacgaacgaa
    13081 gaaaaacaat cgttaatgta ccacgcgcct aaagaatctc gtcccttctg ccagcaccgt
    13141 tataacttag cgcgcacaca cttaaaaaga actattttgg cgctgccaga aagcaacgtt
    13201 attcatgccg ggtatggtag ctatgccgtg attgaggtgg acttagacgg aggagataag
    13261 gcattttact ttgttgcgtt cagggctttc agggaaaaga aaaaactccg tttgcatgta
    13321 actagcgctt atcccatttc tgaaaaacag aaaggtaaat cagtgaaatt tttcaccatt
    13381 gcctacaact tattgagaaa taagcagctt cctcagccct caaaataaca aaacccacct
    13441 taaggtgggt ttcgccagag aattatctct ggtattcaga acgccattac cggactttgc
    13501 cttgaccttg cgataatcgc aggttgcggg atgtctgaat ttcttcagtc tgctgcatcc
    13561 tggaagatga gaacatgtgt tcttattttc gtctctatca tagttgagta tttactctct
    13621 tacaatcaga tctctttcat tgctcaacag gcgatggctt cagactttgc attacggaat
    13681 ttttaagaaa ggcagggcga aacgaggaag aagcttttcg ccctggacaa cattcctgct
    13741 gacggcacta ccataaaaaa acagaacgct ttcaacaacc ttgcgttttt catcttccgt
    13801 agcggctcgg aataaattta cgttcttaca ttccgtctca tagtctttgc gaggcccgtc
    13861 ccagatagct gtcccagtct ttccataccg gctgcaaacc ctgagcggtt aacgcggcag
    13921 caaccgcttc cggtctgcga tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat
    13981 cggcatagcc acctggctgc gttttcgaga aggcgctgac gttattgatc gccagcggaa
    14041 taacgcgatc gcgaaaccac ggtgattccc gcgtggagag tgacagttca atctccggtg
    14101 caagcagtcg gaaggcgcag atggtttgca ctaactggcg ttcatccata atcgacgcag
    14161 gctcaatgcc gccagtacac gggcgcaggc gcggaaagga gacagagtaa cggctttgcc
    14221 agtaatgctg ttgcagccat agcaaatgtt cggcaaccat atagcagtca acgcgccagt
    14281 tgtcggaaag gccaattagc gcgccgaggc ctatcttatc aatccccgca cgccccagcc
    14341 gatccggcgt ttccagccgc cagaagaagt cctgtttttt gcctttcaga tgatggcggg
    14401 cataagtcgc ctcgtgatat gtctcctgat aaaccatcac gccatccaga ccaagctgct
    14461 ttaactcggc gtattccgtc tccgccagcg gttgcacttc catttgtagt gaagagaact
    14521 gttcacgcag ggcggggaga tgacgacgaa agtaatccat ccccactttc gcctgatgtt
    14581 caccagtgac taatagcaga tgttcaaagc ccatctcccg tatagcggcg ctttccctgg
    14641 caatatccgc ttcatccagc gttttgcgct tgatgcgatt actcatggaa aatccgcagt
    14701 acgtgcagtc gttagcgcaa agattggaaa gataaagcgg gacgtagaaa ctaactgtgt
    14761 tgccaaatcg ctgacgggtc agacgctgcg cccgttgggc cagttgttcc agatagccac
    14821 tggcggctgg cgataacagc gccatcatat cgtcgcgggt gaattgcgag gcatttagcg
    14881 cccgctctac gtcaacagcc gttttgccgt tgatacgcag gcggatgtca tcccagtcca
    14941 gttgtcgcca gcgatcgctg aaggttttca tgccgatgcc tccagaaatc cagtcagcgg
    15001 gctggtggca tgagcaaaat gactgcggct gcccggtccg gactgacgtg ccagcaggcc
    15061 tgcttctacc gccagacgaa atgccttcgc catgttgacg ggatcgtccg ctacggcaat
    15121 cgccgtattc actaacaccg cgtcggcccc catttccagg gcctgcgcgg catggctggg
    15181 aacgccgatg ccagcatcga caaccaccgg aactgtggcc tgctggataa taatctccag
    15241 catggcacag gtttccagtc cctgattcga gccaatcggc gcgccgagcg gcatcaccgc
    15301 tgcacagccg acttcttcca gacgtttaca caataccgga tcggccccgc agtaaggcag
    15361 cacgacaaat ccctgttgta ccagcatttc ggcggctttc agagtttcga tgggatcggg
    15421 caacagccag cgggcgtcag ggtgaatctc caattttagc cagttagtgc ctaacgcttc
    15481 acgtgccaga tgggcggcga aaatggcttc ttccgctgtt ttcgccccgg atgtatttgg
    15541 cagcagggtc acacccgccg cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa
    15601 gtcgacacgt ttcatcgcca gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac
    15661 catcagttgt gaagaagcga atttccctgt gccggtaaac agatgtgaat caaacgtttt
    15721 gtccgcaata cgtaacattt caaccccctg caataacctg aaaaagcagg atctggtcgc
    15781 catcctgcac gatatgttgc gcccactgct cacgcgggac gatttgctga ttaatcgcca
    15841 gagccgcgcc cgcttgtcgt tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg
    15901 cggcgcactg catcggttga tcgttaaaca ggatctgcat tgcttcctcc gcataccggg
    15961 caaccactgg cgcggcgcaa cgccaggctg cgccactggc tcgatttacc gtcgaacagt
    16021 cggagttctc ccgcaggtgt ctctataccg cttaataact taatggcttc cagtgcctgc
    16081 aaagtgccca taaccccgac caccgggcca accacgcccg ccgtgcggca gttgcgttct
    16141 ggctcctggt tatctggcca caggcagcgg taacacccct gctcccaggg cggcgtcagt
    16201 accatcaact gaccgccaaa tccgaccgcg ctggcggtga taagcggcgt gttgagtgcc
    16261 acgcaggcgg cattaatctc ctggcgagtc gccatattgt cggtacagtc gagcaccaca
    16321 tcggcccgtg caaccgcatc ttttaacgcc tcacccgtta accgttgttg taatgctgtc
    16381 agttgaatgt cgggattcaa ctgtgtcagt cgctgttggc tgacctgcga tttcgggcga
    16441 tcgatatctt cagtggtaaa gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg
    16501 tcatctgcca gtaccagcgt cccgacgcca gcgcccgcca ggtacagcgc agcaggtgta
    16561 cccagcccgc ccagaccgat aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc
    16621 agagcgatat cgtcgagcag gatttggcgg ctataacgca taaagtcacg gtcattcatc
    16681 gccaactcct gcaatttcca gcaactgtgc cgttgccaaa cgccagtctg cagcttgagt
    16741 aatggcgctg acgacggcga tactgccgac acccgttgct atcaccgcag gcgcgcgtgc
    16801 cagactgata ccgccaatcg ccacggtggg ataatccgcc agtcgctcaa catgccgtgc
    16861 cagctgttcc agcccctgcg gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg
    16921 tcccagcgcg atataagagg ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg
    16981 tgtcgaaacg cccagccgca ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa
    17041 atcttcctgc cccaaatgga cgccatacgc ctgatgcttg atcgccagcc gccagtaatc
    17101 gttgataaac aatcgcgcgt tatagcggcg gcccagcgca attgccgcca cgacatcggc
    17161 ttccacctct tcatcgcgcc gatctttgat gcgtagctgg agagtacgta cgcctgcatc
    17221 caacagacgt tcgatccact gtacgctgtc caccaccggg tacagtccta aacgaaaagg
    17281 tacaggagga aaatcaggct gatacatcac gcttcctcct tacgcaggta gatttctccg
    17341 cctctggcac ggaagttctc cgacatatcc gccattccca tttcaatagt ttgcgtggcg
    17401 gcgtaatcac gcacttcctg gctgattttc atcgagcaaa atttcggtcc acacatggag
    17461 caaaaatggg cgactttgcc ggactcttgg ggcagggttt catcgtgata agcgcgggcg
    17521 gtaaacgggt cgagggccag attaaactgg tcttcccagc gaaattcgaa gcgggctttc
    17581 gacatggcgt tatcgcgaat ttgcgcgccc ggatgccctt tcgccaggtc agcggcgtgg
    17641 gcggcaatct tataggtgat aagcccctgc ttaacatctt ctttattagg cagacccaga
    17701 tgctcttttg gcgttacgta acagagcatc gcgcagccaa accagccaat catcgccgca
    17761 ccaatccccg acgtgaagtg gtcatagccc ggcgcaatat cggtagttag cggccccaga
    17821 gtgtaaaacg gcgcttcgtg gcagtgctct aactcctcgg tcatattgcg gcggatcatc
    17881 tgcatcggca cgtggcctgg gccttcaatc atcacctgca cgtcatattc ccaggcaatt
    17941 ttggtcagtt cgcccagcgt atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga
    18001 atagaaccgg ggcgcagacc gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt
    18061 tcacaaattt cgcggaagtg ttgatagagg aaattttcct gatgatggga gaggcaccat
    18121 ttcgccataa tcgaaccgcc gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc
    18181 acatagcgca gcagtacgcc cgcatggata gtgaagtaat ccacaccttg ctcggcctgt
    18241 tccagcagcg tgtcgcggaa cgcttcccag gtaagatctt cggcgatccc gttaaccttc
    18301 tccagcgcct ggtagatcgg cactgtaccg atcggcaccg ggctgttacg caaaatccac
    18361 tcgcgggttt cgtgaatata gcgaccggtg gagagatcca tcaccgtatc cgctccccag
    18421 cgcgtggacc ataccagctt ttccacttct tcttcgatgg aagaggtgac cgccgagttg
    18481 ccgatattgg cgttaacttt taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc
    18541 ggatgattaa tgttggccgg gataatcgca cgtccggcag caacttcatc acggacaaat
    18601 tccgcagtga tattttccgg cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt
    18661 aaaacctcgc tacggatgcg ctcgcggccc atattctcgc ggatggcgat gaattccatt
    18721 tccggcgtga tgatgccctg gcgggcgtag tgcagttggg tgacacggcg tcctgctttg
    18781 gcgcgttttg gtgttagtac gccgctaaaa cgcagttcgt cgaggccatc atctgccagc
    18841 cgcgctttag tgtaatcgga actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg
    18901 atccacggct ggcgtagttt tgccagccct tgctgcacgt taatggcaat ctgcggatca
    18961 ccatacgggc cggaggtgtc gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt
    19021 tctttgctac cgccaattag cgtcgggcta agctggatct cacgcatcgg cacgcgcacg
    19081 ccggggtgtg tgccagtgat ataaatgcgt tttgagttgg gaaaggcggt gccttccagg
    19141 gtgtcgataa aatgttgggc ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac
    19201 atagctcatt ccaaaaagtt aaggacgtgg cttgtcagac gacggatgaa gcaagagacg
    19261 atcgccgcag gggcgatgcg atagcagatt aactcttgtt cccttcgcag gtattagcct
    19321 gatcaggttc cgcggatccc gaataaacgg tctcagccag ttaaggcact ccgacaagaa
    19381 attagccccg caaatggggc attgaatgta aattacgcgt taacagcgca gaactcaagc
    19441 aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc agcttatctt ccagcacaaa
    19501 gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt tgaaactcaa ggtaattatc
    19561 atgatcgatg gcggtttcca gactggaatc gtagtaatcc ataatctgtt gagtattggc
    19621 ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc ccgttgcctt ccagcttatg
    19681 aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag taatcgacca ggctctgaca
    19741 aaaatcatca agggcttttt cgtttagcct catgtacgat tctttgccag gcttaatgcc
    19801 aaccagattg tagtaagcca cgagcagatg cttacgtaca tgtagccagc gatcaaccag
    19861 tttgttactt cctctgacgc gctccgtcag gttatcgagc tggttaagca tgattgactc
    19921 cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa aaaccatgtt aaacatgcca
    19981 gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt gaaaaattag atcacggctg
    20041 gtgggtcgtc agccatgaac aaaaattatg gttgccgaag ggagaattgc catatggcga
    20101 agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa atcggcgaat ggcaggggga
    20161 acctgtttgg ttagtacaac agcagcggcg tcacgatatg gggtcggtac gtcaggtcat
    20221 tgatctcgat gttgggctgt ttcaactggc cggacgaggc gtacaactgg cggagtttta
    20281 ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg tatccgagca aaaccgaatg
    20341 ggcgatgctg tgcagccatt gccgtgagcg ttactacccg caaatcgccc cctgcattat
    20401 tgttgccatc cgtcgcgatg attcgatcct cctcgcccag catacccgcc atcgtaacgg
    20461 tgtccataca gtacttgccg gattcgtcga agtgggcgaa accctcgagc aggcagtcgc
    20521 gcgggaagtg atggaagaga gcggaattaa agttaaaaac ttgcgttacg tgacttctca
    20581 gccgtggccg tttcctcagt ctttaatgac cgcgtttatg gcggaatatg acagcggcga
    20641 catcgtgatc gacccgaaag aattgctcga ggcgaactgg tatcgctatg acgatttgcc
    20701 gttactcccg ccgcccggca ccgtagcgcg ccgtctgata gaagatacgg tggcgatgtg
    20761 tcgggcagag tatgagtgat gatacactga ccgcctgacg cactaaggaa cagccaaaat
    20821 gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg cgccagcccg ttgatgtcac
    20881 tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg gaatataaag ccacgcgcgc
    20941 ccaggcgggc gattttatgt cgctgtgcaa aaacgccgag ctggcgtgcg aagtgacttt
    21001 gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc ttttccgata tcctcaccgt
    21061 gccggacgcg atggggttag ggctctattt tgaagccgga gaaggtccgc gttttacctc
    21121 gccagtcacc tgcaaagccg acgtcgataa actgccaatt ccggacccgg aagatgaact
    21181 gggttacgtg atgaacgcgg tgcgtaccat tcgtcgcgaa ctgaaaggcg aagtgccgct
    21241 gattggtttt tccggcagcc cgtggacgct ggcgacctac atggtggaag gcggcagcag
    21301 caaagccttc accgtgatca aaaaaatgat gtatgccgat ccgcaggcgc tgcacgctct
    21361 gctcgataaa ctggcgaaaa gcgtcacttt gtatctgaat gcgcagatta aagccggtgc
    21421 tcaggcagtg atgattttcg acacctgggg cggcgtgctt accgggcgcg attatcaaca
    21481 gttctcgctc tattacatgc ataaaattgt tgatggttta gtgcgtgaaa acgacggtcg
    21541 ccgcgtaccg gtcacgctgt ttaccaaagg cggcggacag tggctggaag ccatggcaga
    21601 aaccggttgc gatgcgctgg gcctcgactg gacaacggac atcgccgatg cgcgccgccg
    21661 tgtgggcaat aaagtcgcgt tgcagggtaa tatggatccg tcgatgctgt acgcgccgcc
    21721 tgcccgcatt gaagaagaag tagcgactat acttgcaggt ttcggtcacg gcgaaggtca
    21781 tgtctttaac cttggtcacg gcattcatca ggatgtgccg ccagaacatg ctggcgtgtt
    21841 cgtggaggca gtgcatcgac tgtctgaaca gtatcaccgc taaggagtga ttatggatct
    21901 cgcgtcatta cgcgctcaac aaatcgaact ggcttcttct gtgatccgcg aggatcgact
    21961 cgataaagat ccaccggatc tgatcgccgg agccgatgtc gggtttgagc agggcggaga
    22021 agtgacgcga gcggcgatgg tgctgctgaa atatccctcg cttgagctgg tggagtataa
    22081 agttgcccgc atcgccacca ccatgcctta cattccaggt tttctttcct tccgcgaata
    22141 tcctgcgctg ctggcagcgt gggagatgct gtcgcaaaag ccggatttag tgtttgtcga
    22201 tggtcatggg atctcgcatc ctcgccgtct tggcgtcgcc agccattttg gcttattggt
    22261 ggatgtgccg accattggcg tggcgaaaaa acgtctctgc ggtaaattcg aaccgctctc
    22321 cagcgaaccg ggcgcgctgg ccccgctgat ggataaaggc gagcagctgg cctgggtctg
    22381 gcgcagcaaa gcgcgctgta acccgttgtt tatcgctacc ggccatcggg tcagcgtgga
    22441 cagcgcgctg gcgtgggtac aacgctgcat gaaaggctat cgtctgccgg agccaacgcg
    22501 ctgggcggac gcggtggcct cggaacgtcc ggcgttcgtg cgctatacag caaatcagcc
    22561 ctaattcagg taaactgcgg ataatttccg tatttgagaa ctcaacatgt tacaaaaccc
    22621 aattcatctg cgtctggagc gcctggaaag ctggcagcat gtcactttca tggcttgctt
    22681 atgcgaacgc atgtacccca attacgccat gttctgccag caaaccggtt ttggtgatgg
    22741 gcaaatttac cgtcgtattc tcgatctcat ctgggaaacg ctgaccgtta aagacgcaaa
    22801 agtaaatttc gacagccaac tggagaaatt tgaagaagcg attccttcag ccgacgattt
    22861 cgatctgtac ggcgtttatc cggcaatcga tgcctgcgtg gcgttaagtg aactggtcca
    22921 ttcgcgtttg agtggtgaaa cgctcgaaca cgcggtggaa gtgagtaaga cctccatcac
    22981 gaccgttgcg atgctggaaa tgactcaggc tggtcgcgaa atgagcgatg aagagcttaa
    23041 agaaaaccca gctgtagagc aagaatggga cattcagtgg gaaatattcc gacttttagc
    23101 tgagtgcgaa gaacgcgata tcgagctgat aaaaggcctt agggcagacc tgcgtgaggc
    23161 gggtgagagc aatattggta taatttttca gcaataagac cagaaaacgt gatttaacgc
    23221 ctgatttgtc gtacctggag tcttcccttt cgccccccgt ctggtctaca tttggggggc
    23281 gaaaaaaagt ggctatcggt gcgtgtatgc aggagagtgc tattctggca tttccgtcgc
    23341 actcgatgct tagcaagcga taaacacatt gtaaggataa cttatgaaca agactcaact
    23401 gattgatgta attgcagaga aagcagaact gtccaaaacc caggctaaag ctgctctgga
    23461 gtccactctg gctgcaatta ctgagtctct gaaagaaggc gatgctgtac aactggttgg
    23521 tttcggtacc ttcaaagtga accaccgcgc tgagcgtact ggccgcaacc cgcagaccgg
    23581 taaagaaatc aaaattgccg cagctaacgt accggcattt gtttctggca aggcactgaa
    23641 agacgcagtt aagtaagatt gcgtggcagt gaacagtttt aacgaagggg tggtttcacc
    23701 ccttttgtct ttctggcgtc gatcattgat gctggcaggt gcgctgtttc tcactgcctg
    23761 tagccacaac tcttcacttc ctccctttac cgccagtgga tttgctgaag accagggcgc
    23821 ggtacgcatc tggcgaaaag acagcggcga taatgtgcat ctgcttgccg tgtttagccc
    23881 gtggcgcagt ggcgatacca cgacgcgaga gtatcgctgg cagggcgata acctcacgct
    23941 catcaatatc aatgtttaca gcaaaccgcc ggtgaacatt cgcgcgcgtt ttgacgatcg
    24001 cggtgatctg agctttatgc aacgtgaatc cgatggggaa aagcagcagc tttctaacga
    24061 ccaaatcgat ttataccgtt atcgtgctga tcagatccgc cagattagcg atgccttacg
    24121 tcaggggaga gtcgtgctgc gccaggggcg ctggcatgcg atggaacaga ccgtgaccac
    24181 ctgcgaaggg caaaccatta aacctgattt agattcgcag gcgatagcgc atatcgagcg
    24241 ccgccagagc cgctcttctg ttgatgtcag cgtggcatgg ctggaagcgc ccgaaggttc
    24301 gcaattactg ttagtggcaa actctgattt ctgtcgctgg caacccaacg agaaaacgtt
    24361 ctgatttacc agtggcccat acccatatga ccgccaccac cgcagccgcc gtagcccatg
    24421 cccattccgg ctccgcgcgg aatacccgct tcagccatcg cgatatctcg tttcacccgt
    24481 aactcatcta acgactgacg caaattctcc atctctttgg cgaccgcgtt aattttgctg
    24541 ctatccggtg ggttcgcggc taacagggca ttgtattcat aacgcttcgt caccagttgc
    24601 tgttgcagtg cgctgctttg agcgtaaaag tcattatgga ttttctgcca cgctgtctgt
    24661 tgttcgctgg tcaaaggcgc ggcattttgc tgccacatac cgtgtccgcc gtgagcaaat
    24721 gcagatgtcg atcccatcgc cattgctgaa agcgccatca ttaccagggc aattttcgtg
    24781 ttccgtttca tggttaatcc tccagtggtt gtcttacttc gggtattgca tcttccgtgc
    24841 caacgatgaa acgctgatat gacgggtaat ctggcatgat aaacgagtaa aaatgactcg
    24901 cctgctgcgg gtagcgagtc atttttactc attgaaactt aagcctttgt gttacagcgc
    24961 agggtaagcg ctgataaaag atggcatgat ttctgctgtc agaaagggat gagcaggcaa
    25021 agaagaagat gcgttttatg caacgttcta aagactcctt agctaaatgg ttaagcgcga
    25081 tcctccccgt ggtcattgtt gggctggtag ggttgtttgc ggtgacggtg attcgcgatt
    25141 atgggcgcga gactgccgcc gccagacaaa cgctgctgga aaaaggcagt gtacttattc
    25201 gcgctcttga atccggctcg cgcgtcggca tggggatgcg catgcatcat gcgcagcagc
    25261 aggccttact ggaagaaatg gccgggcagc ctggagtacg ttggtttgcg gtcacggatg
    25321 aacaaggaac aatcgtgatg catagcaact ccggcatggt gggaaaacag ctttattccc
    25381 cgcaggaaat gcagcagtta catcctggtg atgaagaagc gtggcggcgg atcgatagcg
    25441 cagacggcga gcctgttctg gaaatttatc gccagtttca accgatgttt gctgctggaa
    25501 tgcaccggat gcgccatatg cagcagtatg ccgcgacacc acaagcaatt ttcatcgctt
    25561 tcgacgccag taatattgtg agtgccgaag atcgtgagca gagaaacacc ctgattatcc
    25621 tcttcgccct ggcgacggtc ttgctggcaa gcgtattgtc attcttctgg tatcgccgct
    25681 atctgcgctc gcgccagctt ctacaagatg aaatgaagcg caaagagaag ctggtggcgc
    25741 tggggcatct tgcggcaggc gttgcccacg aaatccgtaa cccactttcc tcgattaaag
    25801 gactggcgaa atactttgcc gaacgcgcgc cagcaggggg agaagcgcat caattggcgc
    25861 aggtgatggc gaaagaagcc gaccgtttaa accgcgtggt aagcgagttg ctggaactgg
    25921 ttaagccaac gcatctggct ttgcaggcgg tggatctcaa cacgctgatt aaccactcat
    25981 tacagctggt aagccaggat gcaaacagcc gggagatcca gttacgcttt accgccaacg
    26041 acacattacc ggaaattcag gccgatccgg acaggctgac tcaggtcctg ttgaatctct
    26101 atctcaatgc tattcaggcg attggtcagc atggcgtgat tagcgtgacg gtcagcgaaa
    26161 gcggcgcggg cgtgaaaatc agcgttaccg acagcggtaa gggaattgcg gcagatcagc
    26221 ttgaagccat cttcactccg tacttcacca ccaaagccga aggcaccgga ttggggctgg
    26281 cggtcgtgca taatattgtt gaacaacacg gtggtacaat tcaggtcgca agccaggagg
    26341 gaaaaggctc aacgttcacc ctctggcttc cggtcaatat tacgcgtaag gacccacaag
    26401 gatgacgcac gataatatcg atattctggt ggtggatgat gacattagcc actgcactat
    26461 tttgcaggct ttactgcgcg gctggggcta taacgtcgcg ctggcgaaca gcgggcgaca
    26521 ggcgctggag caggtgcggg aacaggtttt tgatcttgtg ctttgcgatg tgcgaatggc
    26581 ggagatggac ggcatcgcca cgctgaaaga gatcaaagcg ttaaacccgg caattccggt
    26641 gctgattatg actgcgtact ccagcgtcga gacggcggta gaggcactga aaactggggc
    26701 gctggattat ctcatcaagc cgctggattt cgataaccta caggcgacgc tggaaaaagc
    26761 gctcgcgcat acgcacagta ttgatgctga aacacctgcg gtgactgcca gccagttcgg
    26821 tatggtcggt aaaagcccgg cgatgcaaca cctgctcagt gaaatcgccc tcgtcgcgcc
    26881 atcggaagcc acggtactga tccacggcga ttccggcacc ggtaaagagc tggtcgccag
    26941 ggcgattcac gccagtagcg cacgtagcga aaaaccgctg gtaacgctca actgtgcggc
    27001 actcaacgaa tccttactgg aatctgaatt gttcggtcac gaaaaagggg cgtttactgg
    27061 agccgataaa cgccgggaag ggcgctttgt tgaggcggac ggcggcacgc tgtttctcga
    27121 tgaaattggc gatatctcgc cgatgatgca ggtgcgtctg ctgcgtgcga ttcaggagcg
    27181 cgaagttcag cgtgtcggta gcaaccagac tatctcggtt gatgtccggc tgattgcggc
    27241 gacccatcgc gatcttgccg cagaggtgaa tgccgggcgt tttcgccagg atctctacta
    27301 tcgcctgaat gtggtggcga ttgaagtacc atcgctgcgg caacggcggg aagatatccc
    27361 tctgctggct ggccattttc tgcagcgctt tgccgagcgt aatcgcaagg cggtaaaagg
    27421 ttttacgccc caggcgatgg atctgttgat tcattacgac tggccgggaa atattcgtga
    27481 gctggaaaac gcggtggaac gggcagtggt gctgctgacc ggggaatata tttccgaacg
    27541 cgagctgccg ctggcgattg ccagtacgcc gatcccgctg ggacaaagtc aggatattca
    27601 gccgttggtg gaagtggaaa aagaggtgat tctggcggcg ctggagaaaa cgggcggcaa
    27661 caaaaccgaa gccgcccgtc agttagggat cacgcgcaaa acgctattgg caaaactgtc
    27721 gcgttagttc tgctcgcgtt cgatagcgcg ccagccgata tctttccggc agaagcagtc
    27781 gtcccagtga atatcggtca ttaaggcata agcgcgtttc tgcgcttctg ccacggtatg
    27841 acccagcgcg gtgacgcaca gtacgcgccc gccgctggtc actacctgct cgtcatccgc
    27901 cagttttgtg cctgcatgga acactttgcc gtcttccact tcttccagcg gcaggccgtg
    27961 gatcacatca ccggtgcggt aatctcccgg atatccaccc gcagccatca ccacgccgag
    28021 agaagcgcgt tcatcccact cggatgtttt ctcgtccagc ttcccttcac aggctgccag
    28081 gcagagctca accagatcgg acttcatgcg cagcataatc ggctgggttt ccggatcgcc
    28141 aaagcggcag ttaaattcga taaccttcgg attgccctgt ttgtcgatca tcaggcccgc
    28201 gtagagaaaa ccggtgtagg tgttgccttc cgccgccatg cctttcacgg ttggccagat
    28261 gatacgttcc atggtgcgct gatgaacttc atcggttact accggcgcgg gggagtaagc
    28321 gcccatcccg ccggtgtttg gtccggtatc tttatcgcct acgcgtttgt gatcctggct
    28381 ggtagccatc ggcagcacat gctctccgtc caccatcacg ataaagctcg cttcttcgcc
    28441 atcgaggaac tcttcgataa cgatgcgatg acccgcgtcg ccaaaagcgt tgcccgccag
    28501 catatcgtga acagccgctt ccgcttcttc cagcgtcatc gccacgataa cgcctttccc
    28561 ggcagccaga ccgtccgctt taatgacgat tggcgcgcct ttctcacgca gatacgccag
    28621 cgcaggttct acctcggtga agttctggta ttccgccgta gggatcttat ggcgggccag
    28681 gaaatctttg gtaaacgctt ttgagccttc cagttgggcc gcacctgcgg ttgggccgaa
    28741 gattttcagc ccggcggcgc ggaaggtatc gaccacgcct ttcaccagcg gcgcttccgg
    28801 gccgacgatg gtcagatcaa tcttttcgtt ttgtgcgaaa tccaacagcg ccgggatatc
    28861 ggttacgccg atagcgacgt tctgtagcgc gggttccagc gcagtgcctg cattacccgg
    28921 agcaacaaaa acagtttcaa ccagcggcga ctgcgccgct ttccaggcca gcgcgtgctc
    28981 gcgcccgccg ttaccaatca ctaatacttt catctattgc tccattaatg gcggaagtgg
    29041 cgcatgtcgg tgaagagcat cgcaataccg tgctcgtcgg cggcggcaat cacttcgtca
    29101 tcacggatag aaccgccagg ctggattacg caggtcacgc ccgcagcggc ggcggcatca
    29161 ataccgtcgc ggaacgggaa gaacgcgtca gaagccatcg aggaaccttt cacttccagg
    29221 ccttcatcgg ccgctttaat accggcgatt tttgcggagt acacgcggct catctggccc
    29281 gcgccaatgc cgatagtcat attgtttttg gcatagacga tagcgttgga tttcacaaac
    29341 ttcgccacct tccagcagaa cagcgcatca cgcagttcct gttcgctcgg ctgacgtttg
    29401 gtcaccacgc gcagttcttc cgcaccgacc atccccaggt cacgatcctg aaccagcaga
    29461 ccgccgttca cgcgtttgaa atcgaggccc ggaacacgct cgccccactg accgcaggtc
    29521 agaacgcgta cgttctgttt ggcggcggtg attttcaggg cttcttcgct ggcggacggc
    29581 gcaataatca cttcgacaaa ctgacgagaa atgatggcct gtgcggtttc cgcatccagc
    29641 tcgcggttaa aggcaatgat gccgccgaat gcggaggttg ggtcggtttt gtacgcgcga
    29701 tcgtaagcat caagaatgga attgccgata gccacgccgc aagggttggc gtgcttcaca
    29761 atcacacatg ccggctcggc gaactctttc acgcactcca gcgccgcatc ggtatcggcg
    29821 atgttgttat aagagagggc tttaccctga acctgggttg cggtagcaac ggaggcttct
    29881 ttcacattct cttctatata gaaggcagcc tgctggtggc tgttctcgcc gtaacgcata
    29941 tccagcttct taatgaagtt caggttcagc gtgcgtggga agcgaccggc ggcttctttg
    30001 ctttcaccgt ggtaagccgg aaccatgctg ccgaagtagt tggcaatcat gctgtcgtag
    30061 gcggcagtgt gttcgaaggc tttgatggcg aggtcgaaac gggttgcaag cgtcagcgat
    30121 ccttcgttgt catccatctc tttaataatg gcgtcgtagt cgctgctctt caccacgatt
    30181 gcgacatctt tatggttctt ggcggcggag cgcaccatcg ttgggccgcc gatatcgatg
    30241 ttctcaaccg catcttccag cgagcaacct tcacgggcca cggtctgggc gaacggatac
    30301 aggttaacaa ccaccatatc gataggctgg atctgatgtt cttccataat ggcatcgtcc
    30361 tggccgcgac ggcccagaat gccaccatgt actttcggat gcagggtctt cacgcgtcca
    30421 tccatcatct ccgggaaacc ggtgtaatcg gaaacttcgg ttaccggcag acctttttct
    30481 gctaacagac gggcagtgcc ccctgtagac agcagctcca caccgcgtgc ggaaagtgcc
    30541 tgggcgaatt cgacgatacc ggctttgtca gaaacactga gcagagcgcg gcggactgga
    30601 cgacgttgtt gcatggtaaa tcccctggat ttgactatta cagagagcgt tagctgaatt
    30661 tttcgcgaaa aactcagcta acgcccctaa cggggcatcc ttatttttcg cccgcattgt
    30721 aacgaaaacg tttgcgcaac gctcgcgaat ttttctcttt caatggtgat cacaattttg
    30781 acagtggtta ccgtgggaaa aatacagaaa ttacattgat gattgtggat aactctgtgc
    30841 gtaaaagggt ataaagcggg gttttgctgg ggaatgcagc agtcagttat ttttctgcaa
    30901 tttttctatt gcggcctgcg gagaactccc tataatgcgc ctccatcgac acggcggatg
    30961 tgaatcactt cacacaaaca gccggttcgg ttgaagagaa aaatcctgaa attca
//
LOCUS       DAJOWB010000046        30915 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJOWB010000046 DAJOWB010000000
VERSION     DAJOWB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.046"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(163..831)
                     /gene="artM"
                     /locus_tag="OHO85_002275"
     CDS             complement(163..831)
                     /gene="artM"
                     /locus_tag="OHO85_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCQ2671641.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(831..1547)
                     /gene="artQ"
                     /locus_tag="OHO85_002276"
     CDS             complement(831..1547)
                     /gene="artQ"
                     /locus_tag="OHO85_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCQ2671642.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1554..2285)
                     /gene="artJ"
                     /locus_tag="OHO85_002277"
     CDS             complement(1554..2285)
                     /gene="artJ"
                     /locus_tag="OHO85_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2671643.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2303..3031)
                     /gene="artP"
                     /locus_tag="OHO85_002278"
     CDS             complement(2303..3031)
                     /gene="artP"
                     /locus_tag="OHO85_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCQ2671644.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3249..3764)
                     /locus_tag="OHO85_002279"
     CDS             complement(3249..3764)
                     /locus_tag="OHO85_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCQ2671645.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKKGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3890..4213
                     /locus_tag="OHO85_002280"
     CDS             3890..4213
                     /locus_tag="OHO85_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCQ2671646.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4210..5040
                     /gene="amiD"
                     /locus_tag="OHO85_002281"
     CDS             4210..5040
                     /gene="amiD"
                     /locus_tag="OHO85_002281"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCQ2671647.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(5037..6050)
                     /locus_tag="OHO85_002282"
     CDS             complement(5037..6050)
                     /locus_tag="OHO85_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCQ2671648.1"
                     /translation="MKVLVTGATSGLGRNAVESLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6149..7579)
                     /locus_tag="OHO85_002283"
     CDS             complement(6149..7579)
                     /locus_tag="OHO85_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCQ2671649.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(7590..8591)
                     /gene="ltaE"
                     /locus_tag="OHO85_002284"
     CDS             complement(7590..8591)
                     /gene="ltaE"
                     /locus_tag="OHO85_002284"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCQ2671650.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(8628..10346)
                     /gene="poxB"
                     /locus_tag="OHO85_002285"
     CDS             complement(8628..10346)
                     /gene="poxB"
                     /locus_tag="OHO85_002285"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCQ2671651.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(10479..11447)
                     /gene="hcr"
                     /locus_tag="OHO85_002286"
     CDS             complement(10479..11447)
                     /gene="hcr"
                     /locus_tag="OHO85_002286"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCQ2671652.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(11459..13111)
                     /gene="hcp"
                     /locus_tag="OHO85_002287"
                     /gene_synonym="priS"
     CDS             complement(11459..13111)
                     /gene="hcp"
                     /locus_tag="OHO85_002287"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCQ2671653.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(13255..14154)
                     /gene="lysO"
                     /locus_tag="OHO85_002288"
     CDS             complement(13255..14154)
                     /gene="lysO"
                     /locus_tag="OHO85_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCQ2671654.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(14649..15344)
                     /gene="aqpZ"
                     /locus_tag="OHO85_002289"
     CDS             complement(14649..15344)
                     /gene="aqpZ"
                     /locus_tag="OHO85_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCQ2671655.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGTTDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            15770..17428
                     /locus_tag="OHO85_002290"
     CDS             15770..17428
                     /locus_tag="OHO85_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCQ2671656.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(17425..18381)
                     /locus_tag="OHO85_002291"
     CDS             complement(17425..18381)
                     /locus_tag="OHO85_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCQ2671657.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            18532..19647
                     /gene="macA"
                     /locus_tag="OHO85_002292"
     CDS             18532..19647
                     /gene="macA"
                     /locus_tag="OHO85_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCQ2671658.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQELDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            19644..21590
                     /gene="macB"
                     /locus_tag="OHO85_002293"
     CDS             19644..21590
                     /gene="macB"
                     /locus_tag="OHO85_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCQ2671659.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(21663..21887)
                     /gene="cspD"
                     /locus_tag="OHO85_002294"
     CDS             complement(21663..21887)
                     /gene="cspD"
                     /locus_tag="OHO85_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCQ2671660.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            22210..22530
                     /gene="clpS"
                     /locus_tag="OHO85_002295"
     CDS             22210..22530
                     /gene="clpS"
                     /locus_tag="OHO85_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCQ2671661.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            22561..24837
                     /gene="clpA"
                     /locus_tag="OHO85_002296"
     CDS             22561..24837
                     /gene="clpA"
                     /locus_tag="OHO85_002296"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCQ2671662.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(25181..25268)
                     /locus_tag="OHO85_002297"
     tRNA            complement(25181..25268)
                     /locus_tag="OHO85_002297"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25232..25234),aa:Ser,seq:gga)
     gene            25502..26440
                     /gene="ghrA"
                     /locus_tag="OHO85_002298"
     CDS             25502..26440
                     /gene="ghrA"
                     /locus_tag="OHO85_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCQ2671663.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGACLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGEKVCGQVDRARGY"
     gene            26495..27232
                     /gene="ycdX"
                     /locus_tag="OHO85_002299"
     CDS             26495..27232
                     /gene="ycdX"
                     /locus_tag="OHO85_002299"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCQ2671664.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            27256..27810
                     /gene="ycdY"
                     /locus_tag="OHO85_002300"
     CDS             27256..27810
                     /gene="ycdY"
                     /locus_tag="OHO85_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCQ2671665.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            27912..28403
                     /locus_tag="OHO85_002301"
     CDS             27912..28403
                     /locus_tag="OHO85_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCQ2671666.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(28467..29300)
                     /gene="csgG"
                     /locus_tag="OHO85_002302"
     CDS             complement(28467..29300)
                     /gene="csgG"
                     /locus_tag="OHO85_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HCQ2671667.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(29327..29743)
                     /gene="csgF"
                     /locus_tag="OHO85_002303"
     CDS             complement(29327..29743)
                     /gene="csgF"
                     /locus_tag="OHO85_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCQ2671668.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(29768..30157)
                     /gene="csgE"
                     /locus_tag="OHO85_002304"
     CDS             complement(29768..30157)
                     /gene="csgE"
                     /locus_tag="OHO85_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCQ2671669.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(30162..30812)
                     /gene="csgD"
                     /locus_tag="OHO85_002305"
     CDS             complement(30162..30812)
                     /gene="csgD"
                     /locus_tag="OHO85_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCQ2671670.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         7594 a         7731 c         8194 g         7396 t
ORIGIN      
        1 accttagtga gttgtccgtc ttcattataa gaatttatgc actatgtagg ccggataagg
       61 cgtccacgcc gcatccggca caggcaccgt gctgatgtct gatgcgacgc tggcgcgtct
      121 tatcagacct acaaaccccc ccggcgaatg tacgcagcta cattaatttc gccgttcgaa
      181 tgccagtgct ttgcgctcga tcagacgcat catcagcgtc agcaggccgt tgacgaccag
      241 gtaaataatc cctgccgcac cgaacaccat tacatcgtag gtgcgtccgt acaacaactg
      301 gctgtatccc atcacttcca tcagcgtaat ggtgtaagcc agagaggtac ttttgaacac
      361 cagcaccact tcgttggaat aagaagagag cgagcgttta aaggcatacg gcagcaggat
      421 cgccagcgta tcttttttgc tcattcccag ggcgctacag gactgccact gaccttctgg
      481 gatcgcacga attgcaccgt aaaacagctg cgtggtatac gccgcactgt tcagcgataa
      541 cgcaatcagc gcacataacc acggttctga caacaaatgc cacagtgccg gatactcctg
      601 caaagtcgga aactggcccg gcccgtaata aatcaggaag atctgcacca gcagcggcgt
      661 accggtaaac agcgtgatat aaccccgcac cagccacacc agcaccggcg ttttcagcgt
      721 caggatgatg gtaaaaatca atgccagaat cagtgccaca atcagcgagg caacggttag
      781 cgtcaggctg gtgtgtagcc ctttcatcag ttcgggtaaa tactcaaaca ttagctgggc
      841 ctccgctcaa aacgtgtcgc gcgcaggtca atgcgtttga gaatgtactg actgagcagg
      901 gtgatcacca ggtaaatcgc cgccgccaca atgtaccagg taaatggttc ctgggtacga
      961 gtagcgatgc tttttgtttg cagcattaaa tcattcacac taatcaaact gaccagcgca
     1021 gtatctttca gcagcaccag ccactggtta ccgaggccag gcagcgcatg acgccacatc
     1081 tgcggcatca ccagacggaa aaagatagcc gatttcgaca gccccagcgc ctgaccagac
     1141 tcccactgac ccaccggcac cgctttcaac gcgccccgaa gcgtttgcga ggcataggcg
     1201 gcatacagga gtgacagagc gatgacacca caaaggaacg ggctaacgtc gaagttctca
     1261 atgtccatct gcactgggat ctgcacgaac ccaagattga tagtgaagcc atccgaaagc
     1321 gtcagcagca gctgcgagga gccaaaatag ataaacagca ccaccagaat ttctggcagg
     1381 ccacgcagaa tggttaccag cgctgaacct gcccacgcga caggacgcca ttttgccgac
     1441 tcccataccg caaagaacat cgccagcgcc agcccgacaa tcaatgcaca aacggcaagg
     1501 ccgacggtca tcccggcggc gcttgctaaa ggaaaaaatt cattcatcag gaattacttc
     1561 tggaaccatt tgttgtagat ggtttcgtaa gtgccatctt tcttcacttt ttccagcgca
     1621 gtgttgagtt tctgctgcag ctcagtgttg ccctgacgta ccgcgatgcc gaggccagtg
     1681 ccgaagtaat ctttatcggt cactttgtcg cccaccgccg ccagtttcgg gttatctttc
     1741 agccactcag tgaccactgc ggtgtcaccg aagacgccgt cgatacgccc gttttgcaga
     1801 tccagttttg cgttctggta gctgtcatac ggaacggtag tgatttccgg gtgcttatcc
     1861 ataatgaatt tctggtgtgt cgtcccgttc tgtacgccga cttttttgcc tttcagctga
     1921 tcaacactgg tgtatttgcc ttgctgaccc acaaacaggg cagagttgtc atagtacggg
     1981 gtggtaaaca gtacctgctt ttcacgctcc ggagtgatat ccatgcccgc catcactgct
     2041 tctacgcgac ggaatttcag gcttgggatc aggctgtcaa acgcctggtt agagaaagta
     2101 caggttgcat caatctcttt acacagcgcc tgtgccaggt cgacgtcaaa accaacgatc
     2161 tggttgtttg catcaatcga ttcaaacggc ggataggagg cttcggtagc aaaacgaatg
     2221 gtttcggcag ctgtggcgga aagactaaaa cctgcaatta acgcggcaat cagaactttt
     2281 ttcattgttg ttatcccgaa tcttagtgag agagatagtt tttaaatgct tcggtttgcg
     2341 gctcggtaaa gcagctcgcg tcgccttgtt ctacgatatg accattttcc atatacacca
     2401 ctcggctggc ggttttacgc gccacttcaa cttcgtgggt gacgatcacc tgggtaatat
     2461 tcgtttctgc cagctcacga atgatgctga cgatttgtgc cgtaatttcc gggtccagtg
     2521 cggcggtcgg ttcatcgaac agcagtacct gcggttccat catcaacgca cgggcaatag
     2581 caacacgctg ctgctgacca ccagaaagat gcagcgggta acgatcgcta taaggtttga
     2641 gacgcagacg ttccagcagt ttttctgcac gggccagcgc ctgatcttta ctcaacccca
     2701 gtacacggca gggcgcttca atcaggtttt gctgcacggt cagatgcggc cacaggttgt
     2761 attgctgaaa caccatgcca acgttacgac gcaaatcgcg aatcgctttg tcagagggtg
     2821 ttttggtgaa atcgaaatgg ttgcctgcaa tgttgagcgt accggagcgc ggcatctcaa
     2881 gcagattgag tacacgcagc agcgagcttt tacccgcgcc gctggggcca agtaacacca
     2941 gcgtttcgcc ctgtgggcaa tccagcgtga tatcgaacag cgcctgatgc gcgccgtaga
     3001 agcaattaat gccgtttaat tgaatactca ttgacactcg tatactggca gtctgatagc
     3061 tattgaggtc gaagatagta cctttgacag aataattatg caatatttct gctttaaaag
     3121 ttaaaagcaa agcgcattat tcaataaaca tagcacaaaa taacgggggc ggtggtcggc
     3181 gagcataaat gtcggcattc ctcacgaaat gccggacaat ttacggggtt tattggttga
     3241 tcaaggcgtt agcgattctc gatggactga cggagcgtac ccgccgtggc atgaacgctg
     3301 ccgcctaaat agcgtacatc gtcgatcacc caacactggc cttcctgaat cattaacact
     3361 tcatcctgcc agctctgatc accctttttt agatccacac gcagaggaat atttcgcgca
     3421 tcacgattcg ggattgttga ggcactggca acacgggcgc tctctggcaa ggttgtgcga
     3481 ctggagaatg gatcgttggt cagtagttcc cgatggttat tatcccggga ggcatcgcta
     3541 agcagtgtcg ccagtttgtc gctcagataa gggcgcaagg cggtgatgtc gttgctgcgg
     3601 tgcaaaatgc ggtagtcata aaattgctgg gccacgttat ccgggcctcc ttcaacgcaa
     3661 ggaccactgc gtgtgccgtt atctttataa gctggagtga ctgtggtgca ggcactgagg
     3721 agcagtgcgc aggggataag cattgtcaat ttgctgtagc gcataatgat ttccttataa
     3781 gcgatcgctc tgaaagcgtt ctacgataat aatgatatcc tttcaataat agcgtatcag
     3841 tctgataatg cttttgagat cgaaggctta gcaaacaagg agatcgatca tgcaattttc
     3901 tacaacccca actctggaag gccagaccat cgttgaatat tgcggtgtgg tgaccggcga
     3961 agcgatttta ggtgccaata ttttccgtga tttctttgcc ggtatccgcg atatcgttgg
     4021 cggacgttcc ggtgcgtatg aaaaagaact gcgtaaagca cgggagatcg cctttgagga
     4081 attaggctcc caggcgcggg cgctgggggc cgatgccgtc gtcggtattg atatcgacta
     4141 cgaaacggtc gggcaaaacg gcagtatgct gatggttagc gtcagcggta cggcggtgaa
     4201 aacgcgtcga tgagaagagt cttctggctg gtcgctgccg ctctgttatt ggcagggtgt
     4261 gcaggcgaaa aaggcattgt cgagaaagag ggatatcagc ttgatacccg acgccaggcg
     4321 caggcggcgt atccgcgcat taaagtgctg gtgatccact acaccgcaga tgattttgat
     4381 agctcgctgg cgacactgac cgataagcag gtcagctcgc attatctggt ccctgcggta
     4441 ccaccgcgat acaacggtaa accgcgcatc tggcaactgg tgccggaaca agaactggcc
     4501 tggcatgcgg ggattagcgc ctggcgcggg gcaacgcgcc ttaacgacac ctctattggc
     4561 attgagctgg aaaaccgtgg ctggcaaaaa tcggccggag tgaaatattt tgccccgttt
     4621 gaaccggcac agattcaggc gcttattcca ctggcgaaag atattattgc ccgttatcac
     4681 atcaagccgg aaaacgtagt ggcacatgcg gatatcgcac cgcagcgcaa agacgatccg
     4741 gggccattat ttccctggca gcaactggcg cagcagggga ttggtgcctg gccggatgcg
     4801 cagcgggtta acttttacct tgccgggcgc gcgccgcaca caccggtaga taccgcgtca
     4861 ttgctggagc ttttggcgcg ctacggttat gacgttaaac ctgatatgac accgcgcgag
     4921 cagcggcgcg tgattatggc attccagatg catttccgcc cgacgttata taacggcgaa
     4981 gcggatgcag aaactcaggc gattgccgaa gcattgctgg agaaatacgg gcaggattag
     5041 cgcggcagtt ttccgtggtc gcgtagccag gcggcagttt tctcgatacc ttcatccagg
     5101 gtgatgaccg gctgataacc taactcttcc tgcgcacgcg taatatccag cgtaaagtca
     5161 aaattcaact tggagacgcc gtagtgggtc agcggcggct cttttgctga cttgcggcct
     5221 aaacgctcca tgctgcgggc gatcatatcc agcatcgggt aggggacgga acgaatacga
     5281 cagtcaatat tcaactcgtc gatcagcttc tgcacgatgc tgcgcagtgt gcgatgctcg
     5341 ccgttggtga tgttgtacac acgcccggaa ggtagcttat cgcaggcttc ctggcttgcc
     5401 agccacattg cgtgcacggc attttcatag taggtcatat cgaccagcgc actgccgcca
     5461 tgcggtaaca gaatactgcc gtagtggtgc atcatatgcg ccagacgggg aataaagact
     5521 ttatcgtgcg gtccgaacag actttgtggg cgcagaatag taaagcgcgt ttgtggattc
     5581 gcctgcgaaa gcatattgat cacttcttcg ctggctgctt tgctgcgggc aaactcgttg
     5641 gcgaagcggt gagggcgaaa atcttcttta atatcgcgat ggtggtgata atcgaagtac
     5701 agggaggggg aagagatatg aataaagtta cgcacacccc aggcgacagc ccattcaccc
     5761 aggcggcgag tggcgcgaac gttagccaga tcgaaagcct gttgtgtccc ccagggtgag
     5821 gtaaagctgg agcagtgcca cagcgtatca atgcccgcga gcatcacttt agcttgtgat
     5881 gaaaccagct cggtcagatc cgccggaaca aactctgcgc ccattttttc cagcaatttg
     5941 cccattgcct cgttgcgacc ggtcgctcgc acgctgatgc ctttctggca taaagactct
     6001 accgcgtttc gacctaagcc gctggtggcg ccggtaacca gtaccttcat atcaatccac
     6061 tgttgttgag aaaataacgt gcgcattctt ccgtgatttc ccccatgatg caatgggaaa
     6121 catgaaagaa taacgcaggt tttgtcgatt aatctgtgct ttgttctgcc agtctggcga
     6181 tttgttttgc cattccgcga aaaataaaca gatgcgcggg gatcatcaat aaccagtaaa
     6241 acagccccgg cataccgtgc ggatgccaga aagcgcggac atcgatagta cgatagtcgc
     6301 ctttatcttc caggctaaaa cacagtcgtc ccagccccgg cgctttcatg ccaaataaca
     6361 acgtaagttg tttttccggt tcaacgacaa tcactttcca gctatctacc gcatcgccag
     6421 tctgtaaata ttcgcgctcc gggcggcctt tcgccagctt atgaccgatc gcgcggtcca
     6481 tcaacgcccg tgtctgccac aaaatattgc caaagaaata acgctcttta ccgccgattt
     6541 ggttcactac ctgccataaa gcagcaaggc tggcggacgt tttaacggta aaccccgcct
     6601 gtttggcaaa ataaccgtac tccggtcgcc agcgggcaaa ggcctgagcg tcgtagcccc
     6661 agtcgctgga gttgaccagt ttttcctcct ctttcaacgt gctacgtacc gcgtcatcga
     6721 aagcgatcag ccgttgtggg atgagtgcac gtagcgcggt atcatccgcc agcagatcgt
     6781 gtttcagccc ctgaatcaac gccctggcgg tggtgggcgg tacggaagta atcacattga
     6841 gaaaccacac cgaaatccag cgggtgggga gggggatggg gatcaaccag cggcgcttac
     6901 cgctcaccgc cataaaatgt tcaaactgtt gctgataact gagcacctct ggtccggcgg
     6961 cttcgaagat gcggtgttcg ctggccggat gatctaacaa cgccaccaga tagtgaagca
     7021 agttttccag cgcgatgggc gtggtgcgtg aacgtaccca gcgtggcggc gttaacactg
     7081 gcaggttgta gaccatatcg cgcatgactt cgaacgccgc tgaacctgcg ccaacgataa
     7141 ttccggcccg aagttcggtc acaggtacat tcgcttcacg aagaatgtcc gccgtagcct
     7201 gacgagcacg cagatgatcc gactgctcat gtggcggggc ctgcaacgaa ctgagaaaga
     7261 ttaattgctt aactggtact tcacgtagcg catcgcggac gttgagagcc acctggcgct
     7321 cctgagcgat aaaatcgccg ccttcgccca tgctgtgcac cagaaaatag acggtatcga
     7381 tatcctgcaa cagggccgga aggttatccg gccagctgag atcgacttta tggcaactga
     7441 cgtttgccag ttgcagcttt gcaagcctgt cgacatgacg tgccgccgcc aagatctgat
     7501 gcccttgctg gctgagtgtg cgcaccagat gctgaccaat gtagccactg gcaccgagaa
     7561 ctaaaatgcg ttgcggcacg tctctctcct taacgcgcca ggaatgcgcg ccagtgggcg
     7621 gcgacttccg ccagttgttc gcgcgagacg tcaagatgcg tcaccaggcg gacaatcggc
     7681 gaggcgttaa tcagcacgtt tctcgctttc atgtattcgc ctaacgcggc agcattttct
     7741 tccccgacgc gaacaaacag catattagtg tcctgacgca tcacatccgc gcctgcttca
     7801 cgcagttgct ccgccatcca ggcggcgttg tcgtgatctt cctgcaaccg tgcgacgtta
     7861 tttttcaggg catacatccc ggcggcagcc agaatcccgg actgacgcat cccgccaccg
     7921 gccattttcc gccagcggat ggcacgttta atgtaatcac gattaccgac gagtaatgaa
     7981 ccgactggcg tcccaagacc tttcgacagg caaatggtga acgaatcaca atattgcgtg
     8041 atctctttca gttcgcagcc gtaagccacc acggcattaa agatgcgcgc accgtcaaca
     8101 tgcagcgcca gattgcgctc gcgggtaaat tcccatgctt ctttcaggta ttcacgcggc
     8161 agcactttgc cgttgtgggt gttttccaga ctgagtaatt tggtgcgggc gaaatggata
     8221 tcgtcgggtt tgattttcat cgccacttta tccagcggta gcgtgccgtc ggcagccgcg
     8281 tcgatgggtt gcggctgaat actgcccagc accgctgcgc caccggcttc aaacagatag
     8341 ttatgcgcgg cctgaccgac aatatactct tcgccacgtt cgcagtgact gagcagagcg
     8401 accaggttgg cctgagtgcc ggtaggcaga aaaatggcgg cttctttacc ggaaagctct
     8461 gctgcatagt cctgcagagc attaacggta gggtcgtctc cgtaaacgtc gtccccaacc
     8521 ggggcggcca tcatcgcttc gagcatggcg cggctcggtc gggtaacggt atcactgcgt
     8581 aaatcaatca tggcatgtcc ttattatgac gggaaatgcc acccttttta ccttagccag
     8641 ttcgttttcg ccagttcgat cacttcatcg ccgcgtccgc tgataattgc gcgcaacata
     8701 tacaggctaa aacctttggc ctgttcgagt ttgatctgcg gtggaatggc taactcttct
     8761 ttggcgacca ccacatccac caacaccgga ccgtcgatag agaaggcgcg ttgcagggct
     8821 tcatcaactt cagacgcttt ttctacacgg atacccgtaa tgccgcacgc ttcggcaatg
     8881 cgggcaaagt ttgtgtcgtg tagttcggtg ccgtcagtca aatagccacc agctttcatc
     8941 tccatcgcca caaagcccag cacgctgttg ttaaagacga caattttcac tggcagtttc
     9001 atctgcacta ctgagaggaa atcgcccatc aacatgctaa aaccgccatc gccgcacatg
     9061 gcgaccacct gacgttctgg ctctgtcgcc tgcgcaccca gcgcctgcgg catggcgtta
     9121 gccatcgaac cgtggttaaa cgaacctaac aggcgacgct tgccgttcat ttttagataa
     9181 cgtgccgccc acaccgttgg cgtaccaacg tcacaggtga aaatagcgtc atcggcggca
     9241 aaatgactaa tttgctgcgc cagatattgc gggtgaatgg ctttctcgct cggtttagct
     9301 aaatcgtcca gccctttgcg ggcgtcgcgg taatcttcca gcgctttatc cagaaacttg
     9361 cgatcggctt tttcttccac caatggaagc aatgcacgca gagtcgactt gatatcgccg
     9421 accagtgcca tatccacctt gctgtgagcg ccgatgctgg ctgggttgat atcaatctga
     9481 atgattttgg catcggtcgg gtagaaggcg cggtagggaa attgcgtgcc gagtagcact
     9541 aacgtgtcgg cgttcatcat ggtatggaaa cctgacgaga agccgattaa cccggtcatt
     9601 ccaacatcat acggattatc gtattcgaca tgttctttac cgcgcagggc atgaacaata
     9661 ggcgctttaa ttttcccggc aaactcaact aactctttat gcgcccccgc gcagccgctg
     9721 ccacacatca gggcgatatt gctggaataa cgcagcagtt gcgccagttt gcgtaactct
     9781 tcttcttccg gcgtcacgac tggttgtggc gcatgatacc agtgcgtggt tgccccttct
     9841 ggcgcaggtt ttaacgccac gtcgcctggt aacacgacaa ccgaaacgcc acggttaagc
     9901 accgctttgc gcatggcaat cgccagtact tgtgggatct gctccgggct ggaaaccagc
     9961 tcgcaatagt gactacattc gcggaatagc tcttgtgggt gggtttcctg gaaatagccg
    10021 ctgccaattt cgctggaggg aatatgagcg gcaatcgcca gtaccggaac gtgattgcgg
    10081 tggcaatcga acaggccgtt gattaagtgc aggttgccgg ggccgcacga tccggcgcag
    10141 accgccagtt ctccgctaag ttgtgcttca gcgccagcgg caaaggccgc cacttcttcg
    10201 tggcgggtgg acatccactc gatggtgccc atgcgattaa gactgtcact aagaccgttc
    10261 agagagtcgc cagtgactcc ccagatgcgt ttcacccctg ccgattcgag tgttttggcg
    10321 atataagctg caaccgtttg tttcatggtt ctccatctcc tgaatgtgat aacggtaaca
    10381 agtttagttc atctgacgga gggggaaggg atgggagaga aaggaggcac taacggttaa
    10441 atagcccgat gaaaggaata tcatcgggta taaggcgatt atgcgagaac caaatccccc
    10501 tgcggatggc aggagcaggc cagtacgtaa ccttcagcga tttcggcgtc ggtcagcgtc
    10561 attgtactgc tcaccgtata ttcaccggaa accacttttg tcttacagca gccgcaaaca
    10621 cccgcacggc aggcagcgac aaccggaacg ttattgcttt ccagcgcctc cagtagcgtg
    10681 gtgccaaccg gggcgtaaaa ttctcgtgcc ggttgcagtt tggtgaattt cagaccgctg
    10741 gtcgccgctt ctgctactgg ggtgaagaat ttctctttaa agaaacgcgt cacgccgagc
    10801 gctttcactt cctgctctac ccaatccata tacggagccg ggccgcaggt catcacggta
    10861 cgtgaagcta agtcaggtac acctgccagc agttcgcgag tgagacgacc agcgataaag
    10921 ccttcggtaa cgttattttc tgccaccagc gttaccggat agttacgcca ctcatcggcg
    10981 aaaataacat cctgcggcgt acgcacgttg tagatcaccc gcacatcggc ctgtggacgg
    11041 ttcttcgcaa gccagcgacg catcgacata atcggcgtga cgccgcagcc tgccgccagc
    11101 aacaggaatt tatcttctgc tttatcgtcg caggtaaatt cccccatcgc gtccgaaagc
    11161 cagagataat caccgcgttt tacatcgcgc gtcagccact gggagccgac accgtcatca
    11221 atccgccgca cggtcagggt gatatattca ctcacgcctg gcgtggagga aatggtgtaa
    11281 gcacgcagcg tttccgctga gtttcgcacg ctgaccagtg catattgccc ggcgcgatat
    11341 gggtagtaat cgtggcaaat cagggaaatc gtccacacat ccggcgtttc ttgcgtaatg
    11401 tgatgaactt gcatccgcca cgggcattga ttcgttggca tcgtcatcga caaactcctt
    11461 acgcgctcaa cagttgcttc atgtcttctt caacagtggt gatagaacgc aggccaaatt
    11521 tctcgttcag caccgccagc aggtctggtg tcaggaaacc aggtgcagtc gggccggtga
    11581 cgatattttt cacgcccaga gaaagcagcg tcagcagaat gacgatcgct ttctgttcaa
    11641 accaggagag caccagcgac agcggcagat cgttgacacc gcagcccagt ttctctgcca
    11701 gagtgacagc cagaataatc gctgagtaag catcgttaca ttgacctgca tctaccagac
    11761 gcggcagacc ttcgatatcg ccaaactcaa gtttgttaaa gcgatattta ccacaggcga
    11821 gggtcaggat caggcagtca tccggcacgc tggtggcgaa atcggtgaag tagtggcgct
    11881 cgccgcgtgc gccgtcacag ccaccaagca ggaagatatg acgcagtttt tcacggctca
    11941 ccagatcaat cagcgtatcc gctgcgccaa gcagcgtctg gcggccaaag cccacggtga
    12001 tcaggtgcgg aatttcgctg tacgggaagc ccgccatttg ttgcgcctgg gcgatgaccg
    12061 cagaaaaatc ttcgccatcc agatgacgta cacccggcca gccaacgatg ctgcgggtcc
    12121 agatacgatc gtcataagcg cctacggttg ggtcgatgat gcagttcgag gtcatcacga
    12181 tggggccagg gaaacgagcg aactccactt gctgattctg ccagccgctg ccgtagttac
    12241 cgaccagatg cttgaattta cgcagctccg ggtagccatg cgcaggcagc atttcgccgt
    12301 gggtgtaaac attaacgccc gtgccttcgg tctgttccag caggttgtag agatctttga
    12361 gatcgtgacc ggaaatcagg atgcatttac ccgccgtcgc tttgacgttg acctgggttg
    12421 gcgtcgggtg accgtattta ccggtttcgc ctgcatccag aatgctcatc actttgaagt
    12481 tcatctggcc gatttccatt gaacactcaa gaagcgcgtt catatcggca ggccaggtcc
    12541 ccagccacgc catgatttta tgatactggg cataaatatc gttgtcgtat tgaccgagaa
    12601 catgcgcgtg ttccatatag gccgctgcac ctttcaggcc atacaggcac agcagacgca
    12661 ggccgagaat gttttcgcca atcgccgctt tatctttgtt aggggtaaat tctgctgcct
    12721 gacgttgcag ctcgccgaga tcgtcgctca ccagttgcag gtcagccatc gggttatcga
    12781 cgcgcgcgtt ggcatctaca gccaggcatt gcgctttcag cgcctcgcgc agggcaatcg
    12841 cttcacgagc gtagccgaca atacgcggag aatcgaagtt aacgttggtc agggttgaga
    12901 aaaaggcacg tggcgcgaag ctgtcaacat cgtggttgat gatgccgtat tcacgcgctt
    12961 ttaccgccca ggcagaaagc ccttgcagcg ccgcgatgag taaatcctga aggtcagaag
    13021 tttccgccgt tttaccacac atcccctgcg cgtatgagca gccgtttcct gccggagtac
    13081 ggatagtttg ttcacattgc acacaaaaca tgatcacacc ttttaaagtt atatttaata
    13141 tacatgttta aggttaagac gcttaacgcg gggataaaag ggatttttca tgcaacttta
    13201 agggagattg atttagcgca attttggcgg cagggatcta ccgccagaga ggtattacgc
    13261 agagaaaaag gcgatgagga tcggcactaa caggctaaga ataaaaccgt gaacaattgc
    13321 cgccgggacc atatccagcc cgccagtacg ttgaagaacg ggcagggtga aatccattga
    13381 tgtggcaccg cataagccca gtgcagtaga gcggctgcgg cgaatcagcc cagggatcaa
    13441 cataatagca atcagttcac gggccagatc attaaaaaac gccgcgctcc cgattaccgg
    13501 accaaaagat tcggtcaata aaataccgga aagagaatac cagccgaaac cggaggccat
    13561 tgccagcgcg gtattgatgg ggagatcaag aataaaggcg ttaattaaac caccaattaa
    13621 tgaactgaca accaccacca cggcgacaat cattccccgg cgattaagga caatctgctt
    13681 taaggtcatg ccattattgc gcaactgaat accaacgagg aaaagtagca aaattaacgt
    13741 gtattcactg gcttcggtcg cgtgttgtaa gaaagccagt ccactcagac caatggcaaa
    13801 accaatcact actacgccgc acagttttag cgactccagc gccatcgcaa tacgcgacgg
    13861 gagtttttct tgctgatggt ggttgcgcca cggcaggcct cgctccagcc acatcagggc
    13921 ggcaatatta cacagtaaaa taacggtaat actgacggca gaataatgca gaatcgccaa
    13981 caggttactg gcgaggttat cgagaaacgc cagactgata cccataaaaa agagaataag
    14041 gtaaaccatc cagcttaata gctgattaat aacttttaac gcagcttgtt ggcgaagcgg
    14101 aatgaggtaa cccacaatca ggggaaccag aatgattaac agcccagaaa acatgaaaac
    14161 ccagtccttg caaagatgaa gtcgaaatgc gcgatgacac actactgaaa gcggaaggac
    14221 gagtaaagtt gcaattaaaa ggaaatgtta tgcataagga gcagtagagt attcgttttc
    14281 atttaaagat attcttgcgc tttaattaca aactgcaccg atgttggtgg cgtcaaaatc
    14341 gccgaggcgt tccctgaagg ccggggcagc ccacatggat gtgggctgag ggcgcgtttt
    14401 acagggatgt tacctcgcgc ccgacccggt agccgtaagg gataagtcga gggcaccgcg
    14461 cagcggcgat tttgttcgcc agagcccggg ggtgcagggg gcggcggcga ttggccgccc
    14521 cctgcgcgct ctttgcgcca gtggcaatat gtggcttagc tcatgaaagg agcgcaacaa
    14581 gatgatgaat caacatataa caacatctta aaaaaaggcc tgacattacg ccaggccttc
    14641 tgcgttaatt aatcacgctt ttccagcagg gtccggtaaa tcagaccacc gataatgccg
    14701 ccgacaattg gcaccaccca gaagaaccac agttgttcta atgcccagcc gccctggaag
    14761 atagcaaccg cggtgctgcg cgccgggtta acagaagtgt tagtcaccgg aatactaatt
    14821 aagtgaatca gggttaaggc cagaccaata gcgatcggcg caaaacctgc cggcgcgaat
    14881 ttgtcggttg tgccgtggat caccaacagg aaacctgcac tcaataccag ttcaactacc
    14941 agcgcggaaa gcatggaata accgcctggt gaatgctcgc cataaccgtt agaagcaaaa
    15001 ccgctggctg ccgcgtcaaa acccgtttta ccactggcaa ttaaatacag cagcgccgct
    15061 gcaacaatac cgccgacaac ctgggcaatt acgtagccaa cgacttcttt tgccggaaaa
    15121 cgtccgccag cccataaacc aatagtgacc gccgggttaa aatgaccacc agaaatatga
    15181 ccaacagcaa aggccatcgt cagaacggtc agaccgaacg ccaacgccac gccggcaaaa
    15241 ccaatgccta attccgggaa gcctgcggcc agtacagcac taccacagcc accaaaaaca
    15301 agccagaaag taccaaaaca ttcagctgct aattttctga acatatccac ctcaattaaa
    15361 aattgaccct gtgaaaaata tggtcgtttt atagggtcgt cgtaaaaaag tgacgacgga
    15421 aataatgcgc ggctatttta aaaacgaagg cgagtcattc accagataaa taaatccagt
    15481 aaatttgatt tagggcaaca gcgggttgcc ccatatagtc atttgtctga ttgacagtgt
    15541 agtgcaagca aaagatttaa tcctttaggc gtaataaaaa ataatttatc atgctaatta
    15601 tttgattttg ttgtttttgc agacttatca gcaagaggga gtataacgcg attattcgct
    15661 catttttcag acatttgcca tgcttaaatg tgatgtcatc acgtattagc aaggcctttc
    15721 ccgttatact gccagcgtaa aggataagtc acatatttct ggaggggata tgattcttga
    15781 gcgcgttgaa attgtgggtt ttcgcggtat caaccgtttg tcgttgatgc tcgaacaaaa
    15841 caacgtcctg attggggaga acgcgtgggg taaatccagc ttgctggacg ccttaaccct
    15901 gctgctatcg ccagaatcag atctctacca ttttgagcgc gacgatttct ggttcccgcc
    15961 gggagatatc aacgggcgag aacatcatct gcatattatt ttgaccttcc gcgaatcgct
    16021 gccaggccga catcgggttc gccgttatcg gccgctggaa gcgtgctgga cgccatgcac
    16081 cgatggctat caccgtattt tttatcgtct ggaaggggag agtgcggaag acggcagcgt
    16141 gatgacactg cgcagttttc tcgataaaga cggacatccg attgatgtcg aggatattaa
    16201 cgatcaggca cgccatctgg tgcgtttaat gccggtgctg cgcttgcgtg atgcccgttt
    16261 tatgcgccgt attcgtaacg gcacggtgcc aaatgttccc aatgtggaag tcaccgcgcg
    16321 ccagctcgat ttcctcgccc gtgagttgtc ctcacatccg caaaatctct ctgatggaca
    16381 gattcgtcag ggactttccg caatggtgca gttgcttgag cattatttct ctgagcaggg
    16441 ggccggacag gcgcgatatc gtttaatgcg gcggcgggcc agcaatgagc aacgaagctg
    16501 gcgctatctg gacatcatca accggatgat tgaccgacct ggagggcgct cgtatcgggt
    16561 tattttgctc ggcctgtttg ctactttatt gcaggcaaaa ggcacattga gactggataa
    16621 agacgcgcgt ccattgttgc tgatcgaaga tccagaaacc cgtttacacc ccattatgct
    16681 ttcagttgcc tggcatctgt tgaatcttct gccattgcag cgcattgcca ccaccaactc
    16741 gggtgagttg ctttcgttaa cgccggtaga gcatgtttgc cgactggtac gtgagtcctc
    16801 gcgcgttgcc gcctggcgtc tggggccgag tggcttgagt accgaagata gccgacgcat
    16861 atcctttcac attcgtttta accgtccgtc atcgctgttt gctcgctgct ggttgctggt
    16921 ggaaggggaa acggaaacct gggttatcaa tgaactggcg cgccagtgtg gacaccattt
    16981 cgatgccgaa gggatcaagg tcattgagtt tgcccagtcc gggctaaagc cactggttaa
    17041 atttgcccgc cgaatgggga ttgaatggca tgtactggtc gatggcgatg aagcagggaa
    17101 gaaatatgcc gctacggtac gcagcctgtt gaataacgat cgggaagccg aacgagaaca
    17161 tttaacggcg ttaccggcgc tggatatgga acattttatg tatcgccagg gattttccga
    17221 tgtgttccac cgcgtggcgc aaatcccgga aaatgtaccg atgaatctgc gcaaaattat
    17281 ctcgaaagcg atccatcgct cttccaaacc cgatcttgcc attgaagtgg caatggaggc
    17341 cggacgtcgt ggtgtggact ccgtaccgac gctgctgaaa aaaatgttct cacgcgtgct
    17401 gtggctggcg cgcggtcgcg cggattaacc gcgaaacatc gtggccattt gtggctgaat
    17461 agcgtcgagc atctcatagc gccgacggta ttcagcccgt tttttactgg cgatttcggc
    17521 aatctctttt cgtgctatct gtgctggaag gcggtaatgg cgttcagcat cacatacgcc
    17581 gccaaccgat tcccagaaag cgttgtaatc ggcatggatc ttgccttctt tatcacgata
    17641 acgcaggctg cggtaaatat gcgtttcatt gctgacggca ataatctgct ctacctgcaa
    17701 acgttgggca aacagacagg ccgcttccat cacgaggcgt ttgggaaata gcccgtggca
    17761 ggctttcgtc gcattctgga tttcctgatg tggaatttcc cattttgcgc cttgcagtcc
    17821 gccaataaac atcgttcttt tcccctgata ttcacacagg gtaaacgtga tctctgccag
    17881 aggaatacct tcgctgttgc gaaacaggat tgtgctgtca ccttctttat ccattgagat
    17941 catcatggtc agctcaagcg tgaactgctc gccgtttttg ccttccagtt tagccagttg
    18001 cagcccgggg gtattcaaat ataagctgaa ttcttccgcc gacatacatc cgcggagtaa
    18061 cgcataatgg taacgtaacg cctccagcaa ttgcttacgg ctaagattcg ccgcaaggta
    18121 agggcgatgc agacgcacag gcagtcgcgg ttggcgcgtt aacaacacat tgagattagg
    18181 ccagtgggaa agttcgttca tccactcaac gcttaaacgc ggcataatca acgagcgcag
    18241 caaaaatttc tggcgaaaac tacggcgatg ccagaattta cccggccgac actgtccacg
    18301 tgccagacta agaaaaagtg acaggctgct gagagattca gatggcgtaa aggtccgttc
    18361 agttagctgc gacatattca tgaaatcaat ggttatacat gacgtcgatt tcaccattgc
    18421 gtatcttaac caaacatcaa tagtgtgatt actaacgtaa attttagggt tttgttgata
    18481 tttcgttgaa gttaatgacc cggattggca tatggagtat tcagaatatt tatgaaaaag
    18541 cggaaaaccg tgaagaagcg ttacgttatt gcgctggtga tagtcatcgc cggactgatt
    18601 acgttatgga gaattcttaa cgcacccgtg ccgacttatc agacactgat tgtgcgcccc
    18661 ggtgatttac agcaaagcgt gctggcgacc ggaaagctgg acgcgctgcg taaggttgac
    18721 gtgggcgcgc aggtcagcgg tcagttgaaa actctgtcgg tggcgattgg cgataaagta
    18781 aaaaaagacc agcttttagg ggttattgat cctgaacagg ctgaaaacca gattaaggag
    18841 gtcgaagcaa cgctgatgga gctacgtgcg cagcggcagc aggcggaagc ggagctgaaa
    18901 ctggcgcggg tgacgtattc ccgtcagcaa cgtctggcac aaacgcaggc ggtttcacag
    18961 caggagctcg acaccgccgc gacggagatg gctgtgaaac aggcgcaaat tggcaccatt
    19021 gacgcgcaaa tcaagcgcaa tcaggcctct ctcgatacgg ctaaaaccaa tctcgattac
    19081 actcgcatcg ttgccccgat ggccggggaa gtcacgcaaa tcaccactct gcaaggccag
    19141 acggtgattg ccgcacaaca agcaccgaac attctgacgc tggcagatat gagcaccatg
    19201 ctggtaaaag cgcaggtttc tgaagcggat gtaatccacc tgaagccggg gcaaaaagcc
    19261 tggtttacgg tacttggcga tccactgacg cgctacgagg ggcaaatcaa ggatgtacta
    19321 ccgacgccgg aaaaggttaa cgacgctatt ttctattacg cccgttttga agtccccaac
    19381 cccaatggtt tgctgcggct ggatatgact gcgcaagtgc atattcagct caccgatgtg
    19441 aaaaatgtgc tgacgatccc tctgtcggcg ttaggcgatc cggttggcga taatcgttat
    19501 aaagtcaaat tgttgcgtaa tggtgaaaca cgcgagcgtg aagtgacgat tggcgcacgt
    19561 aacgataccg atgttgagat tgtcaaaggg cttgaagcgg gcgatgaagt ggtgattggt
    19621 gaggccaaac caggagctgc acaatgacgc ctttgctcga attaaaggat attcgtcgca
    19681 gctatcctgc cggtgatgag caggttgagg tgctgaaggg catcaccctc gatatttatg
    19741 ccggtgagat ggtagcgatt gttggcgctt caggttccgg taaatcgacc ctgatgaata
    19801 ttctcggctg tctggataag gccaccagcg gcacctatcg cgtcgccggt caggatgttg
    19861 ccacactgga cgccgatgcg ctggcgcaac tgcgccgcga gcatttcggc tttattttcc
    19921 agcgttacca tttactttcg catttaaccg ccgagcagaa cgttgaagta cccgccgtct
    19981 atgctggtct tgagcggaaa cagcgactgc ttcgtgccca ggagttgctg caacggctgg
    20041 ggctggaaga ccgtacagag tattatccgg cacagctttc gggtggtcag caacagcgcg
    20101 tcagcatcgc gcgggcattg atgaacggtg gtcaggtaat tcttgccgat gaaccaaccg
    20161 gcgcactgga cagccattct ggcgaagagg tgatggcgat cctgcatcag ctgcgcgatc
    20221 gtgggcatac ggtgattatc gtcacccacg atccgcaggt cgctgctcag gccgagcggg
    20281 tgatcgaaat tcgcgacggc gaaattgtgc gcaatcctcc cgccattgaa aaagtgaatg
    20341 ttgctggcgg gacggagcct gttgtcaaca cggtgtctgg ctggcggcag tttgtcagcg
    20401 gttttaacga ggcgctgacg atggcatggc gggcgctggc agcgaataaa atgcgtactt
    20461 tactgaccat gctggggatt attatcggta ttgcgtcggt ggtttccatt gtcgtggtgg
    20521 gtgacgccgc caaacaaatg gtgctggcgg atattcgttc tattggtacg aatactattg
    20581 atgtctatcc cgggaaagat tttggcgatg acgatccgca atatcagcag gcgctgaagt
    20641 acgacgactt aatcgccatc caaaaacaac cgtgggtcgc ctcagccaca cctgccgtct
    20701 cgcaaaacct gcgcctgcgt tataacaatg ttgatgttgc tgccagtgcc aatggcgtga
    20761 gcggcgatta ttttaatgtc tatggcatga ccttcagtga aggaaacacc tttaatcagg
    20821 agcagctgaa cggtcgtgcg caggtcgtgg ttctcgacag taatactcgc cgccagcttt
    20881 tcccccataa agcagatgtg gttggcgagg tgattctggt cggcaatatg cccgccagag
    20941 tcattggtgt ggcggaagaa aaacagtcga tgtttggtag cagtaaagtg ctgcgtgtct
    21001 ggctacctta cagcacgatg tccgggcgag ttatgggcca gtcgtggctg aactccatta
    21061 ctgtcagggt aaaagaagga tttgacagcg ccgaggcgga acagcaactc acgcgtttac
    21121 tttcactgcg ccacggaaag aaggatttct ttacctggaa catggacggc gtcttgaaaa
    21181 ctgttgaaaa gaccacacgt actttacaac tgtttctgac gctggtggcg gtgatttcgc
    21241 tggtggtggg cggtattggt gtaatgaata ttatgctggt gtcagtgacc gagcggacgc
    21301 gggaaattgg cattcgcatg gctgtaggtg cgcgagcaag cgatgttttg caacagttcc
    21361 tgatcgaagc cgtactggtt tgcctggtcg gtggcgcgtt gggaataaca ctgtcactgt
    21421 taattgcttt caccttgcag cttttcttac ccggctggga gattggtttt tcaccgttgg
    21481 cactgctgct ggcgtttctc tgctcgacgg tcaccgggat tttatttgga tggttacccg
    21541 cacgaaatgc ggcacgactg gatccagtag atgctctggc acgagagtaa tttttgagat
    21601 aaaaatgcca gccgatcggg ctggcatttt gcctttagga tgtacacaat gagacagaag
    21661 agctatgcga ctgccgcttc tacttcgacg ggcacaataa cactggcgtg attgcctttt
    21721 ggcccctggt ggacatcaaa ctgaacggat tgtccagctt ttagcgttct gtaaccatcc
    21781 atctgaatgg tggaataatg agcgaaaata tcttcgccgc cgccttcagg gcagatgaaa
    21841 ccaaaccctt tggcattgtt gaaccactta acagtaccct tttccatgct tcgacatcct
    21901 tcgcaaatct tatacaagta agatggaata aaccggggtc agagaggggg ctgttcaaaa
    21961 cctcgccaac tctagaaata caatttagag aattagggcg agccgtcaag cgtttgacag
    22021 gggccagggg gcaggtatga atcaaaaatt tgaagcagtt aacgctattg acaggaatgt
    22081 gacagatgtc gctgatgcca acgatagatg atagttatct atcatgtgga gtagattggt
    22141 caggcaaata agctcttgtc agcggcaggg cgttctgccg ataaccgtaa ccgaagatga
    22201 taactgacaa tgggtaaaac gaacgactgg ctggactttg atcaactggc ggaagaaaaa
    22261 gttcgcgacg cgctaaaacc gccatctatg tataaagtga tattagtcaa tgatgattac
    22321 actccgatgg agtttgttat tgacgtgtta caaaaattct tttcttatga tgtagaacgt
    22381 gcaacgcaat tgatgctcgc tgttcactac caggggaagg ccatttgcgg agtctttacc
    22441 gccgaggttg cagaaaccaa agtggcgatg gtgaacaagt acgcgaggga gaatgagcat
    22501 ccattgctgt gtacgctaga aaaagcctga atgcaggcat aaaaattggg ggaggtgcct
    22561 atgctcaatc aagaactgga actcagttta aatatggctt tcgccagagc gcgcgagcac
    22621 cgtcatgagt ttatgaccgt cgagcacttg ttactggcgc tgctcagtaa cccatctgcc
    22681 cgggaggcgc tggaagcgtg ttctgtggat ttggttgcgc tccgtcagga actggaagcc
    22741 tttattgaac aaaccacacc cgttctgcct gccagtgaag aggagcgcga cacacagccg
    22801 acgctgagtt ttcagcgtgt actgcaacgt gcggtcttcc atgtccagtc ctccggtcgc
    22861 aatgaggtaa ccggtgcaaa cgttctggtc gctatcttta gcgaacagga gtcgcaggcg
    22921 gcatatctgt tgcgtaaaca tgaagtcagc cgtctcgatg tggtgaactt tatctctcat
    22981 ggcacgcgta aagacgagcc gacacagtct tctgatcctg gcagccagcc aaacagcgaa
    23041 gaacaagctg gtggggagga acgtatggag aatttcacga cgaacctgaa tcagcttgcg
    23101 cgcgtgggcg gaatcgaccc actgattggt cgtgagaagg agctggagcg tgctattcag
    23161 gttctctgcc gtcgccgtaa aaacaacccg ctgctggtgg gggaatctgg tgtcggtaaa
    23221 accgcgattg cggaaggtct tgcctggcga attgttcagg gcgatgtgcc ggaagtgatg
    23281 gctgactgta cgatttactc tctcgatatc ggttctctgt tagcgggcac taaatatcgc
    23341 ggcgactttg aaaaacgttt taaagcgttg ctcaagcagc tggagcagga cactaacagc
    23401 atcctgttta ttgatgagat ccacaccatt atcggtgcgg gtgcagcgtc tggtggccag
    23461 gtcgatgcgg ctaacctgat caaaccgttg ctctccagcg gtaaaattcg cgtaattggt
    23521 tcgacaacct atcaggagtt cagcaacatt ttcgagaaag accgtgctct ggcgcgtcgc
    23581 ttccagaaaa ttgatattac tgaaccgtcg atcgaagaaa ctgttcaaat catcaatggc
    23641 ctgaaaccga agtatgaagc gcaccacgac gtgcgttata ccgcaaaagc ggtgcgtgca
    23701 gcggtagagc tggcggtgaa atacattaac gatcgtcatc tgccggataa agccattgac
    23761 gttatcgacg aagcgggcgc tcgcgcacgc ctgatgccgg taagcaaacg caagaaaacc
    23821 gttaatgtgg cggatattga gtccgtggtg gcccgtattg cgcgcattcc agagaagagt
    23881 gtttctcaga gtgaccgcga taccctgaaa aacctcggcg atcgcctgaa aatgctggtc
    23941 ttcggtcagg ataaagccat tgaggcgctg actgaagcca ttaagatggc gcgtgcaggt
    24001 ttaggtcacg aacataaacc ggttggttcg ttcctgtttg ccggccctac cggggtcggg
    24061 aaaacagagg tgacggtaca gctttcgaaa gcgttgggca ttgagctgct gcgctttgat
    24121 atgtccgagt atatggaacg ccataccgtc agccgtctga ttggtgcgcc tccgggatac
    24181 gttggttttg atcagggcgg tttgctgact gatgcggtca tcaagcatcc acatgcggtg
    24241 ctgttgctgg acgaaatcga gaaagcgcat ccggacgtgt tcaatattct gttgcaggtg
    24301 atggacaacg gtacgctgac cgataacaac ggacgcaaag cggacttccg taacgtggtg
    24361 ctggtgatga ccaccaacgc cggggtacgt gaaactgagc gtaaatccat tggtcttatc
    24421 caccaggata acagcaccga tgcgatggag gagatcaaga agatctttac accggaattc
    24481 cgtaaccgtc tcgacaacat tatctggttt gatcatctgt caaccgacgt gatccatcag
    24541 gtggtggata aattcatcgt cgagttgcag gttcagctgg atcagaaagg tgtttctctg
    24601 gaagtgagcc aggaagcgcg taactggctg gccgagaaag gttacgaccg ggcaatgggc
    24661 gcacgtccga tggcgcgtgt catccaggac aacctgaaaa aaccgctcgc caacgaactg
    24721 ctgtttggtt cgctggtgga cggcggtcag gtcaccgtcg cgctggataa agagaaaaat
    24781 gagctgactt acggattcca gagtgcacaa aagcacaagg cggaagcagc gcattaatct
    24841 gattgttagg taggttggtc aagtccgtaa tctcgaaaga ggttacggac tttttgttta
    24901 tggggtggag gaggttcaga cccttttttt aatgatgatg gtaagttgtt gataattagt
    24961 gctgcgggaa ggtaaggata aaaaagggtg ctgcaggaga atgggatggt tttgctttat
    25021 taacaacggg ctaaacgtgt agtatttgag ttcactgccg tacaggcagc ttagaaatcg
    25081 acggggtgcg gtaaaacctt tgcgaacgcg ttcactgccg tacagacaga taaaatgcga
    25141 aaaaaaagct cgcactttcg tacgagctct tctttaaata tggcggtgag ggggggattc
    25201 gaacccccga tacgttgccg tatacacact ttccaggcgt gctccttcag ccactcggac
    25261 acctcaccaa attgtcgttc ctgtcttgct ggaacgggcg ctaatttagg gaaatcatga
    25321 cctgaggtca acaaactttt tgaaaaaatc gcgcgtttat tcaaacttca atcaatgtgt
    25381 ggttttaata agcgaaatct gctttttttg ccaccgacca cggatttgtt atgctggtgg
    25441 cctttgtaga tcataacgat aagtgcgaat aaatttcgca caacgctttt cgggagtcag
    25501 tatggatatc atcttttatc acccaacgtt cgatacccaa tggtggattg aggcactgcg
    25561 caaagctatt cctcaggcaa gagtcagagc gtggaaaagc ggagataatg actctgctga
    25621 ttatgcttta gtctggcatc ctcctgttga aatgctggca gggcgcgatc ttaaagcggt
    25681 gttcgcactc ggggccggtg ttgattctat tttgagcaag ctacaggcac accctgaaat
    25741 gctgaaccct tctgttccac tttttcgcct ggaagatacc ggtatgggcg agcaaatgca
    25801 ggaatatgct gtcagtcagg tgctgcattg gtttcgacgt tttgacgatt atcgcatcca
    25861 gcaaaatagt tcgcattggc aaccgctgcc ggaatatcat cgggaagatt ttaccatcgg
    25921 cattttgggc gcaggcgtac tgggcagtaa agttgctcag agtctgcaaa cctggcgctt
    25981 tccgctgcgt tgctggagtc gaacccgtaa atcgtggcct ggcgtgcaaa gctttgccgg
    26041 acgggaagaa ctgtctgcat ttctgagcca atgtcgggta ttgattaatt tgttaccgaa
    26101 tacccctgaa accgtcggca ttattaatca acaattactc gaaaaattac cggatggcgc
    26161 gtatctcctc aacctggcgc gtggtgttca tgttgtggaa gatgacctgc tcgcggcgct
    26221 ggatagcggg aaagttaaag gcgcatgctt ggatgttttt aatcgtgaac ccttaccgcc
    26281 tgaaagtccg ctctggcaac atccacgcgt gacgataaca ccacatgtcg ccgcgattac
    26341 ccgtcccgct gaagctgtgg agtacatttc tcgcactatt gcccagctcg aaaaagggga
    26401 gaaggtctgc gggcaagtcg accgcgcacg cggctactaa taaagcatca ggattcctgc
    26461 tatccttggc gggaattgaa tacaggagag agttatgtat cccgtcgacc ttcatatgca
    26521 taccgttgcc agcacacatg catatagcac attgagtgat tacattgccc aggccaaaca
    26581 aaagggcatt aaactttttg cgatcaccga tcatggcccg gatatggaag atgctccgca
    26641 tcactggcac ttcattaaca tgcgtatctg gccgcgagtg gttgatgggg tagggatcct
    26701 gcgcggcatc gaagctaaca ttaaaaatgt tgatggtgaa attgattgca gcggtaaaat
    26761 gtttgactcg ctggatctga ttattgccgg ttttcatgag ccggtttttg caccacatga
    26821 caaagcgacc aatacacaag cgatgatcgc cactatcgcc agcggcaatg tgcatataat
    26881 aagccatccc ggaaatccca aatatgaaat agatgtgaaa gccgttgctg aagcagccgc
    26941 gaaacatcag gtggcgctgg aaatcaataa ttcttcattt ttacactcac gtaagggcag
    27001 tgaagacaac tgtcgtgcgg tagccgcagc ggtacgtgat gctggaggtt gggtggcatt
    27061 aggctcggat tctcacaccg cgtttaccat gggggaattt gaagagtgtc ttaaaatcct
    27121 cgacgcggta gattttccgc cagagcgcat tttgaatgtt tctccgcgcc gcttactgaa
    27181 cttccttgaa tctcgcggta tggcaccgat tgcggaattt gcagaccttt aattactagt
    27241 aatggaaata tattaatgaa cgagttttct atcctctgtc gtgtgctggg ttcgctctat
    27301 taccgccaac cgcaagatcc tttactggtg ccgctgttta ccctgattcg tgaggggaaa
    27361 ctggctgcga actggccact ggagcaggat gagttactga cgcgtttaca gaaaagctgt
    27421 gatatggcgc aagtctctgc cgattacaat gcgttgttta tcggcgatga atgtgccgtg
    27481 ccgccatatc gtagcgcatg ggttgaggac gcaacggaag cggaagtgcg cgcttttctt
    27541 tccgaacgag ggatgccact ggcggatacg ccagccgatc acatcggcac attgctgctt
    27601 gcggcttcct ggctggaaga tcagtcaacg gaagatgaga gcgaagcact ggaaacactg
    27661 ttcagtgagt atctgttacc ctggtgtggt gcgttcctcg gcaaagtgga ggcccatgca
    27721 accacgcctt tctggcgcac catggcaccg ctaacccgtg atgccattag tgcaatgtgg
    27781 gacgagctgg aagaagattc tgaagagtaa atgtgatcat cgtcactcat aactgcaaaa
    27841 gataatttgt tgttgcataa aatgtgtgct cgatctcatt catggccgcg ttttctgcta
    27901 tcatgcgcgg catgaacata cttctctcta ttgcaatcac aacaggcatt ctctccggta
    27961 tctggggatg ggtggctgtt tccctgggct tactaagctg ggcgggcttc ctgggctgta
    28021 cggcctactt tgcctgcccg caaggtgggc tgaaagggct ggcgatctcc gctgcaaccc
    28081 tgcttagtgg cgtggtgtgg gcgatggtca ttatttacgg tagtgcactg gcaccacatc
    28141 tggaaattct cggttatgtc ataaccggta ttgtggcgtt tctgatgtgt attcaggcca
    28201 aacagctgct gctttcattt gttccgggga cgtttatagg cgcatgcgcg acgtttgccg
    28261 ggcagggtga ctggaaactg gtgttacctt cgctggcgct tgggctggta tttggttacg
    28321 caatgaaaaa cagcggcctg tggctggcgg cgcgtagtgc aaagacggct caccgtgagc
    28381 aggaaatcaa aaataaagcg tgaggggcac tcacgctttc gcttaaacag taaaatgccg
    28441 gatgataatt ccggcttttt tatctgtcag gattccggtg gaaccgacat atggcggtat
    28501 ttcaccagaa tgtcgttctg ccgttctgct ttgttctgca aatcccacag accacggtcg
    28561 ataccatcat taatcaggaa aatgacccct gtttcgatag ccgacatcag acacagcata
    28621 acgggttcgt ttgaggtgta acccacttcc ccttccagca agcgctggta gtcaataaag
    28681 cggaaaaccc cggcctgaac ttcataggaa agtatcgtct tactggtgtt caccgaggag
    28741 aggatctcac cggtactcac attgacgacg cgcaggttca cggcaatctg atcgagctgg
    28801 tattgcgtgt ctgcaccgat cccaaaatat cttgccccaa caccgcccga tttaacgttg
    28861 ctttcataac cgataatcga accttcaacc ataatatttg ccgccgttaa agattgcagc
    28921 gggattcggt tattaatggc aaccgtgccg ttttcttgtg ccgcacgaat aatcttgcgc
    28981 tcgttaagca ggttttgtaa gccctggcgc tccagcggta taaaccagcg agaatctttc
    29041 aatgccgtga ccagcattgc cgtggcgctt tgcggaacag cagtggagaa gttacttgcc
    29101 gggtagggtt taaattgccc ggtttcgtcc tgaatgttgt ataccgaaac aaagatctta
    29161 cccgtcggcg ctggcagatg ggtcaaatct ttgtagctct gagctcgagg cattaatgtc
    29221 ggtctggcgg cttctttagg cggggcggtt aagcatccgc tcagtaacat gacggcaacc
    29281 aaaagaaata agcgctgcat gattattttc cttatgaagc tggggcttaa aaatcggttg
    29341 agttattttg taaacccgaa acctggatgg tcgaggtttg tccggtttta cgatctgtca
    29401 cgttcaactg caattgacca tcgcggttag caatatcaac aatataatcg ttggtcacca
    29461 tgcggcccgg tttaccggta ttaatattcg acagtagccc acctaaaatt tgtgactgga
    29521 tggcctgagt aaagttatct aacgctgagg gtgtttcaat accaaagtca tcgttatagc
    29581 tcggatcttt ataagagttt tgggcctgag cgctatttaa taaaaaagcg ccattatttg
    29641 ggttaccacc aaagtttgga ttacggaact ggaaagtcat ggttccagcc caacttaatg
    29701 gcgaaataag catgagtaga actactgcat gtttgacacg cattgcagcc tccgaacaat
    29761 tttttattta gaattcatca tgcgccaaat cgcccgtact taataacgcc tgatttatct
    29821 ggcgacgatt tagtgcttct tcagtttgaa tcagtgcaaa gacgacagtt ttctcgaagt
    29881 ctcttttcaa cggaaataaa aaagtctgga aaataacgtc ctgattgacc gttatagtga
    29941 tccagcttcc ccatcgtgca ctgggccttt cattaatcgt taagttaccc gtatagtcac
    30001 tttcccattt atcactaaag gctcggtaaa aatcatggcc aatagatgaa acagtatggt
    30061 cagttaacaa tcccgggact tctacctcaa cggcgtgaag attccctgcg gcgaacagaa
    30121 attctgccgc cacaatccag cgtaaataac gtttcatggc tttatcgcct gaggttatcg
    30181 tttgcccagg aaaccgcttg tgtccggttt tttacggcta tcttcttgaa aagattataa
    30241 agatgcgttt taaccgtatt ttcgctgatg aacaacgaac gagcgatctc gttattagac
    30301 gcgccgatac gcagcttatt caggatctct ttttcccgat gagtaaggag ggctgattcc
    30361 gtgctgttat aacgatagtt accagaatgt gtaatcaggt aactggcaag cttttgcgta
    30421 aagtagcatt cgccgcgcag gacgccttgc aacccattga caacacgttc ttgatcctcc
    30481 atggcataaa aaacgccgtt gatatgaggc cagttttcaa tgtcgcggta cgggtaatct
    30541 tcaggcgtat ttagcaacaa tattttgata ttgttgtttt tcctgctcaa agtatcctgc
    30601 caataatgga taagcttttt atctgcttcc atcatatcca gaagaataat agagcctgaa
    30661 gatatatcgt ccagagaacg ttgaatatta tgtaattttc ctgtaattgc cagcgattgt
    30721 ttaaggtgct gcaagagagc tgtcgcttgc aaagacggtt tagtgatcaa caataatgta
    30781 tgaccatgaa tactatggac ttcattaaac atgatgaaac cccgcttttt ttattgatcg
    30841 cacacctgac agctgcctct aaaatagaag caccagaagt actgacagat gttgcactgc
    30901 tgtgtgtagt aataa
//
LOCUS       DAJOWB010000047        29785 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJOWB010000047 DAJOWB010000000
VERSION     DAJOWB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.047"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            41..550
                     /locus_tag="OHO85_002306"
     CDS             41..550
                     /locus_tag="OHO85_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCQ2671671.1"
                     /translation="MILSNNVFADEGHGIVKFKGEVISAPCSIKPGDEDLTVNLGEVA
                     DTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQTDLLKNTKETE
                     IGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPSKDATPGNVYA
                     QADYKIAYE"
     gene            610..3114
                     /locus_tag="OHO85_002307"
     CDS             610..3114
                     /locus_tag="OHO85_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCQ2671672.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3130..3879
                     /locus_tag="OHO85_002308"
     CDS             3130..3879
                     /locus_tag="OHO85_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCQ2671673.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3881..4945
                     /locus_tag="OHO85_002309"
     CDS             3881..4945
                     /locus_tag="OHO85_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2671674.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(4988..5188)
                     /gene="glgS"
                     /locus_tag="OHO85_002310"
     CDS             complement(4988..5188)
                     /gene="glgS"
                     /locus_tag="OHO85_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCQ2671675.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            5457..6086
                     /locus_tag="OHO85_002311"
     CDS             5457..6086
                     /locus_tag="OHO85_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HCQ2671676.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            6113..7774
                     /locus_tag="OHO85_002312"
     CDS             6113..7774
                     /locus_tag="OHO85_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCQ2671677.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            8013..8072
                     /locus_tag="OHO85_002313"
     CDS             8013..8072
                     /locus_tag="OHO85_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCQ2671678.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            8389..8448
                     /gene="ibsE"
                     /locus_tag="OHO85_002314"
     CDS             8389..8448
                     /gene="ibsE"
                     /locus_tag="OHO85_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCQ2671679.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(8568..10001)
                     /gene="hldE"
                     /locus_tag="OHO85_002315"
     CDS             complement(8568..10001)
                     /gene="hldE"
                     /locus_tag="OHO85_002315"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCQ2671680.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(10049..12889)
                     /gene="glnE"
                     /locus_tag="OHO85_002316"
     CDS             complement(10049..12889)
                     /gene="glnE"
                     /locus_tag="OHO85_002316"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCQ2671681.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SISAHPEWLTELESQPPQADEWQHYVAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAELHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(12912..14213)
                     /gene="ygiF"
                     /locus_tag="OHO85_002317"
     CDS             complement(12912..14213)
                     /gene="ygiF"
                     /locus_tag="OHO85_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCQ2671682.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     NTHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVSQ
                     AWLENWQGLHHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            14455..15075
                     /locus_tag="OHO85_002318"
     CDS             14455..15075
                     /locus_tag="OHO85_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCQ2671683.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            15139..16377
                     /gene="cca"
                     /locus_tag="OHO85_002319"
     CDS             15139..16377
                     /gene="cca"
                     /locus_tag="OHO85_002319"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCQ2671684.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPE"
     gene            complement(16467..17288)
                     /gene="bacA"
                     /locus_tag="OHO85_002320"
     CDS             complement(16467..17288)
                     /gene="bacA"
                     /locus_tag="OHO85_002320"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCQ2671685.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTSGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(17378..17746)
                     /gene="folB"
                     /locus_tag="OHO85_002321"
     CDS             complement(17378..17746)
                     /gene="folB"
                     /locus_tag="OHO85_002321"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCQ2671686.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            17851..18468
                     /gene="plsY"
                     /locus_tag="OHO85_002322"
     CDS             17851..18468
                     /gene="plsY"
                     /locus_tag="OHO85_002322"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCQ2671687.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(18481..19413)
                     /gene="ttdR"
                     /locus_tag="OHO85_002323"
                     /gene_synonym="dan"
     CDS             complement(18481..19413)
                     /gene="ttdR"
                     /locus_tag="OHO85_002323"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HCQ2671688.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            19620..20531
                     /gene="ttdA"
                     /locus_tag="OHO85_002324"
     CDS             19620..20531
                     /gene="ttdA"
                     /locus_tag="OHO85_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCQ2671689.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            20528..21133
                     /gene="ttdB"
                     /locus_tag="OHO85_002325"
     CDS             20528..21133
                     /gene="ttdB"
                     /locus_tag="OHO85_002325"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCQ2671690.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            21181..22644
                     /gene="ttdT"
                     /locus_tag="OHO85_002326"
     CDS             21181..22644
                     /gene="ttdT"
                     /locus_tag="OHO85_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCQ2671691.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(22687..23700)
                     /gene="tsaD"
                     /locus_tag="OHO85_002327"
     CDS             complement(22687..23700)
                     /gene="tsaD"
                     /locus_tag="OHO85_002327"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCQ2671692.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            23938..24153
                     /gene="rpsU"
                     /locus_tag="OHO85_002328"
     CDS             23938..24153
                     /gene="rpsU"
                     /locus_tag="OHO85_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCQ2671693.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            24264..26009
                     /gene="dnaG"
                     /locus_tag="OHO85_002329"
     CDS             24264..26009
                     /gene="dnaG"
                     /locus_tag="OHO85_002329"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCQ2671694.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            26204..28045
                     /gene="rpoD"
                     /locus_tag="OHO85_002330"
     CDS             26204..28045
                     /gene="rpoD"
                     /locus_tag="OHO85_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCQ2671695.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHATAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(28124..28630)
                     /gene="mug"
                     /locus_tag="OHO85_002331"
     CDS             complement(28124..28630)
                     /gene="mug"
                     /locus_tag="OHO85_002331"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCQ2671696.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            28755..28830
                     /locus_tag="OHO85_002332"
     tRNA            28755..28830
                     /locus_tag="OHO85_002332"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28788..28790,aa:Ile,seq:cat)
     gene            complement(28884..29648)
                     /gene="nfeF"
                     /locus_tag="OHO85_002333"
     CDS             complement(28884..29648)
                     /gene="nfeF"
                     /locus_tag="OHO85_002333"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCQ2671697.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELVID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLEM
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
BASE COUNT         7585 a         7794 c         7294 g         7112 t
ORIGIN      
        1 gtctgctttt aaaaagtcac ttcttgttgc tggcgtagca atgattcttt ccaataacgt
       61 atttgctgac gaaggtcacg gcattgttaa atttaaaggt gaagttatct ctgctccatg
      121 ttcaattaaa ccaggtgacg aagacttaac tgtaaatctg ggtgaagtag cagataccgt
      181 tctgaaaagc gaccagaaat ctttggctga gcctttcact attcacctgc aagactgcat
      241 gttgagccag ggtggaacca cttattcaaa agcgaaggtt actttcacca cagcgaatac
      301 tatgacgggt cagactgacc tgctgaaaaa caccaaagaa actgaaatcg gtggtgccac
      361 aggcgttggc gtacgtattc tggatagcca gagcggcgaa gtgactttag gtactccggt
      421 agtaattaca ttcaacaata ctaacagcta tcaggaactg aacttcaaag ctcgtatgga
      481 atccccaagc aaagatgcta ccccgggcaa cgtttacgct caggctgact ataagattgc
      541 ttacgagtaa tcacattagg ccgggttcgc ccggccacat cattaacgga ttaatgataa
      601 gtggaacaga tgtataaaaa attaaaatta accacaataa gcgaattgat taaaaatatt
      661 tattgttcat tatccgttct catcattggt tgtgcgtcag cttatgccgt tgaattcaac
      721 aaagatttaa tcgaagccga agatcgtgaa aacgttaacc tttcccaatt tgaaactgat
      781 ggccaattac ccgtaggtaa atactcatta aacgctctta ttaataataa aaggacgcca
      841 atccatctgg acctgcaatg ggtattaatt gataaccaaa ctgcagtttg tctgactcca
      901 gagcaattaa cattattagg ctttactgat gagattattg aagaggctca gcaaaacctg
      961 atcgatggtt gttaccctat cgaaaaagaa aaacaaatta caacttatct cgataaaggg
     1021 aaaatgcaat tatccatatc tgcacctcag gcatggttaa aatacaaaga tgcaaactgg
     1081 acgcctcctg aactttggga tcatggtatt gctggggcat ttcttgacta caatttatat
     1141 gcctctcatt atgcaccaca tcagggcgat aattcacaaa atataagttc ctatgggcag
     1201 gctggggtta atcttggggc ctggcgcctg cgtactgatt accagtacga tcagtcattt
     1261 aacaatggca aaagccaggc gaacaacctg gattttccgc gtatttattt gtttcgccca
     1321 atcccagcaa ttaatgcaaa actaactata ggtcaatacg atactgaatc ctctattttc
     1381 gactctttcc atttttctgg cgtttcgttg aaaagcgatg agaatatgtt gccgcctgat
     1441 ctacgtggtt acgcaccgca aatcacgggg gttgcacaaa cgaatgcaaa ggtcactgtc
     1501 tcacagaaca accgtattat ttatcaagaa aatgttcctc ctggcccatt tgctattact
     1561 aatttattca atacattaca ggggcaactg gacgtcaagg ttgaagaaga ggacgggcaa
     1621 gtcacgcaat ggcaagttgc atctaatagt attccttatc tgacgcgtaa agggcagatt
     1681 cgctacacca ctgctatggg taaaccgacc agcgttggtg gtgattcctt acaacaaccc
     1741 ttcttctgga ctggtgaatt ctcatggggt tggctgaaca atgtatccct gtatggtggt
     1801 tcagttttaa caaaccgtga ttatcaatct ctggctgccg gcgttggctt taaccttaac
     1861 tcattgggtt cattatcttt tgatgtcaca cgatctgatg ctcagttgca taatcaggat
     1921 aaagaaacgg gttatagcta ccgcgctaac tattcaaaac gttttgaatc taccggtagc
     1981 cagctcactt tcgctggtta ccgtttctct gataaaaact ttgtgacaat gaatgaatat
     2041 atcaatgaca ctaaccatta cacgaattat cagaatgaaa aagagagtta tattgtcacg
     2101 tttaaccagt atcttgaatc attaaggtta aatacatacg taagtttggc tcgtaatact
     2161 tactgggacg ccagcagtaa tgtgaattat tcattatcac ttagccgcga ttttgatatc
     2221 gggccattaa aaaacgtctc cacttctcta acatttagcc gaataaactg ggaagaagac
     2281 aaccaggatc aactgtacct aaatatttcg attccctggg gaaccagtag aacattgagc
     2341 tatggtatgc aacgaaatca ggataacaag atttcgcata ctgcttcgtg gtatgactct
     2401 tccgatcgaa ataattcctg gagcgtttct gcttcaggcg acaatgatga attcaaagat
     2461 atgaaagcgt cactacgcgc cagttatcag cataataccg agaacggtcg tctctatctc
     2521 tccgggacat cacagcgaga cagttattat tctctgaatg ccagttggaa tggttcattc
     2581 acggcgactc gtcacggagc cgctttccat gactatagtg gtagtgctga ctcgcgtttt
     2641 atgatcgacg cagatggtgc agaagatatt ccgttgaaca ataaacgcgc ggtaactaat
     2701 cggtatggca tcggagttat tccatcagtc agcagttaca taacaacatc attaagcgtt
     2761 gatacccgaa atcttccaga aaatgtggat atcgaaaact cggttatcac caccacctta
     2821 accgaaggtg ctattggcta cgccaaactt gatacccgca agggctatca aatcatgggg
     2881 gttattcgcc tggcagatgg tagtcaccca ccactgggga ttagcgtaaa agataaaacc
     2941 agccataaag aattaggact ggttgctgat ggcggctttg tatacctcaa cggcattcag
     3001 gatgacagca aacttacttt acgctggggt gataaatcct gttttattca accacccaat
     3061 agcagcaact taaccaccgg aacggttatt ttaccgtgta ttagccaaaa ttaattcagg
     3121 atgaaactaa tgcgctactt aaacacaaag aatctcattg cagcaggagt attactcgcc
     3181 tgcatgagca gcattgcctg gggtgccatc atcccggacc gtacccgtat cataatgaat
     3241 gagtcagata aaggggaagc attaaaactt actaatcaaa gcaaaaatct accctatctg
     3301 gcgcaaacat ggatagaaga tacgaaaggc aataaatcac gtgactttat tgtcacggtt
     3361 ccccctatgg tacgtttaaa tcccagtgag caaattcaaa taagaatgat aagtcaggaa
     3421 aaaattgctc aactgcctaa tgacagagaa acgctcttct attttaacgt gcgagaaata
     3481 ccgccaaaaa cagataaaaa aaatgtgatg caagtcacta tgcagcacgc attgaaacta
     3541 ttctggcgac caaaagctat tgaattagaa gatgatgggg taatgtctta cgaaaaagtt
     3601 gaaatcatcc gtagaaatga tggttcgatc cgctttaaca ataagatgcc ttatcacgtt
     3661 acgctgggtt atatcggaac aaacggaatc acaatgttgc cacaaacgca aagtttaatg
     3721 gtaacccctt atagccatgc agatacgcag tttaaaaatg ttccgtcagc ttttcaggtt
     3781 ggttatatca atgattttgg tggattaagt ttttacgaaa taaactgccc gacggttaat
     3841 aactcttgta atgtttccgt agccaaacga gataaataat atgcgctact tattaattgt
     3901 aattatattt attataggtt ttagtgcatt acctgtatgg gcaatggact gctatgctga
     3961 acatgaaggg ggaaataccg tagtcattgg ttacgtacca agaatttcta tccccagtaa
     4021 tggtaaaaaa ggtgataaga tctggcaaag cagtgagtat tttatgaatg tcttctgtaa
     4081 taatgcatta cctgccccat cacctggaga agaataccca tcagcctgga ctaatataat
     4141 gatgttttta ccaggcggtc aggactttta taatcagaat tcctatattt ttggcgtaac
     4201 atacaatgga gttgattatg attcgactgc ccctaatgct atcgcagcac cagaatgcat
     4261 tgatataaaa ggagcgggta cctttaataa tcactataaa aaccccgctg tctgtagtgg
     4321 aggtcctgag cctcaattat cagtaacctt tccagctcgc gtacaactct atattaaatt
     4381 agcaaaaaat gccaatagag ttaataaaaa cctggtgtta cctgatgaat atatcgctct
     4441 agagtttaaa ggtatgagtg gcgcaggagc tatagaagta gacaaaaatt tgaccttcag
     4501 gattcgtgga ttaaataata ttcatgtcct tgactgcttt gttaatgtcg cactggaacc
     4561 agctgatggc gttgttgact ttggtaaaat aaattcccga acaattaaaa ataccagcgt
     4621 tagtgagacg tttagtgtag tcatgacaaa agatcctggt gcggcctgta ctgagcaatt
     4681 caatatttta gggagctttt tcactacgga tattttgagt gattatagcc atctggatat
     4741 gggtaatgga ctgctattga agatatttca taaagatgga acagcaacgg aatttaaccg
     4801 cttctcacaa tttgcttctt tttcatcgtc tagtgcacct tcggtcaccg caccattcag
     4861 ggcagaactt agtgcgaatc cggcagagac tgttgttgag ggaccgttta gtaaagacgt
     4921 catcctcaaa atcacctata actagcatct aatacaaaca ctaaaacggg ccatcaggcc
     4981 cgttatatca gtgctctaac tccagctctc ttgcctgcat catctgttga caataccagg
     5041 cataacgtgc gcaaaaccag gttctatgtt cttcatccat gttaccagta acggcaagaa
     5101 tatcgaccgg gcgaccttct gcgtgcattc tggcaaaact acgcgccagg aaatcgaaat
     5161 tatttaaaga attaagacta tgatccataa gcacatcctc cggttatcgc atacctttta
     5221 aggccatatg ctttttctga tttcagtatt ccccaatcag tgtagtgcct ttaaacataa
     5281 cgtgcgtaaa tatattgtcc ccgatcacac tttttagtga aaaataaaaa accatattaa
     5341 aaaactttct cttccagaac ttctctaaca tgtgttatta ttaacaaaga taaatgtaag
     5401 caaggaaact atcccaaagt aaatattgcg tcactaaatg gacattggag tgacatatga
     5461 ttttattcgc cgactataat accccttatc ttttcgctat ttcctttgta ctgttgatcg
     5521 gccttttaga aattttagcg cttatctgcg gtcacatgct ctccggtgct cttgatgcac
     5581 atcttgacca ttatgattcc ataaccacag gtcatattag ccaggcactt cattatctca
     5641 atatcggaag attgccagct ctcgtcgttc tctgcttgct ggctggtttt tttggtctta
     5701 ttggcatcct gttgcagcat gcctgcatca tggtctggca atcaccactg tcgaacctgt
     5761 tcgtggttcc tgtcagttta ctgtttacga ttattgcggt gcattatacc ggtaaaatag
     5821 ttgcgccctg gattccacgc gaccatagtt cagctattac agaagaagaa tatattggta
     5881 gcatggcatt aattaccggt catcaagcca cttcaggtaa tccctgcgaa ggaaaactca
     5941 ccgatcaatt tggccaaata cattatttac tactggagcc tgaagaagga aaatttttca
     6001 ctaaaggaga caaagtgtta attatttgcc gactttctgc aacgcgatat ttggcggaaa
     6061 acaatccctg gccacaaata ttgtaattta ttaatcaaaa ggaaatttaa taatggatga
     6121 tattgttaat tctgtgccct cctggatgtt taccgcgatt attgccgtat gcattctgtt
     6181 tattattgga attattttcg ccaggctcta tcgtcgcgct tcggcagagc aagcttttgt
     6241 tcgtactggt ttaggtgggc aaaaagtggt gatgagcggt ggcgcaatcg tgatgccgat
     6301 ctttcatgaa ataatcccca tcaatatgaa tactctgaag ctggaagtca gccgctcaac
     6361 cattgatagc ctaattacga aagatcgtat gcgtgtcgat gtagtagtcg ctttctttgt
     6421 acgggtaaaa ccgtctgtag aaggcatcgc cactgcggcg cagacgttgg ggcaacgcac
     6481 gctatcacca gaagacttac gtatgttggt tgaagataaa tttgtcgatg ccctccgtgc
     6541 aacagctgca caaatgacca tgcatgagtt acaggatacc cgcgagaact tcgtgcaggg
     6601 agtacaaaat actgtcgcag aagacctgtc gaaaaacggt cttgagctgg aaagcgtttc
     6661 acttaccaac tttaaccaga cctcgaaaga acatttcaat ccgaacaatg cctttgacgc
     6721 cgaaggttta accaaactga ctcaggagac ggagcgccgt cgtcgcgaac gtaacgaagt
     6781 tgaacaggat gtagaagttg cggtgcgtga gaaaaaccgt gatgcacttt cgcgcaagtt
     6841 ggagattgaa caacaagaag cgtttatgac gcttgagcag gagcagcagg ttaaaacccg
     6901 tactgccgaa cagaatgcac gtattgcggc ttttgaagct gaacgtcgtc gtgaagcaga
     6961 gcagacgcga attctggctg aacgacaaat tcaggaaacg gagatcgagc gcgagcaggc
     7021 cgtccgctca agaaaggttg aggctgaacg tgaagttcgt attaaagaga tcgaacaaca
     7081 gcaggttacc gaaatcgcca accagacgaa atcgatcgct attgccgcca aatcggaaca
     7141 acagtcccag gcagaagcgc gtgctaacct tgcactcgca gaagcagtaa gtgcgcaaca
     7201 aaacgtagaa accactcgcc agaccgcaga agccgatcgt gctaaacaag ttgccctaat
     7261 cgctgccgcg caggatgcag aaaccaaagc ggttgaactg accgtgcggg cgaaagcaga
     7321 aaaagaagcc gcagagatgc aggcggcggc tatcgttgag ttagccgaag ctacacgtaa
     7381 aaagggtctg gcagaagcag aagcacaacg tgcgctgaac gatgctatca acgtactttc
     7441 tgatgaacaa accagcctta aattcaaact ggccttgttg caggcgctac ctgcggtaat
     7501 agaaaaatcc gttgagccga tgaaatctat tgacggtatc aagattattc aggtcgatgg
     7561 tctgaatcgt ggcagcgctg cgggtgatgc aaacacgggt aatgtggggg gcggaaacct
     7621 ggcggaacaa gcattatcag ccgctctctc ttaccgcaca caggcaccgc tgattgactc
     7681 cttgctcaat gaaattggcg tttcaggcgg ctcactggcg gcattgactt cacccttaac
     7741 ctcaacaact cccgtcgccg aaaacgtaga ataaattttc agctatgaac gtccctggca
     7801 caaaaagccg gggatttttt atatctgcgt tccgctaaaa actgcaaatg atcaggtcgt
     7861 tgcaacgttt tgcgtgaccg ctcggggaag gcaaaactgc ctctgggaaa gcgttgcgcg
     7921 aggtccggcg ctcatcaaca atcgggaagt cagcaaaggg ctgaaacggg aaagcccctc
     7981 ccgaggaagg ggccttacag aaggaaaggg ttatgatgaa gctcgtcatc atactgattg
     8041 tgttgttaat cgtaagtttc gcagcttatt aacagccaat cagagggggg agaaatcctc
     8101 cctcaccctt gttcctttac tttaggttga aaaaaaatca gcgtcaagaa ggtttcatta
     8161 acacatatcc ctggcccaaa aagccgggga ttttttatat ctgcgttccg ctaaaaggtg
     8221 caaatgctca ggacgttgca gcgttttgcg tgaccgctcg gggaaggcaa aactgcttca
     8281 gggaaagcgt tgcgcggggt ccggcgctca tcaacaatcg gggagtcagc aaggggctga
     8341 aacgggaaag cccctcccga ggaaggggcc ttatagaagg aaagggttat gatgaagttc
     8401 gtcatcatac tggttgtgtt gttactgtta agtttcccga cttactaaca actgatcaga
     8461 ggggggagaa atcctccctt acccttgttc ctttacttta ggttgaaaaa acaacagcgt
     8521 caataggcct gcctgctacg aagcgagatc tgtgaaccgc tttccggtta gcctttttta
     8581 tcctgttgga tcttcttgat gatgttagtg gtcgagcaac cgtcttcgaa gttgagcacc
     8641 agcacttcgc cgccgttggc ccagacttct ttactcccgg cgatctcttc tggtttatag
     8701 tcgccgccct tcaccagtaa atctggcagg atcccggcga tcaagcgctg cggcgtgtct
     8761 tcttcaaacg acaccaccca gtcgaccgct tccagtgcgc ccagcacaat catacgctgt
     8821 tcgagcgggt taacggggcg ggaatcccct ttcagccgtt tggtggaggc atcgctgttc
     8881 acagcgacaa tcagacggtc acccagtttg cgagcatttg ccagatagga gacgtgcccg
     8941 gcgtgcagga tgtcaaagac accgttggtc attaccacct tttcgccgcg tttacgcgct
     9001 gccgctacag caagcttcag ttcctcttcg gtcatcacac caaagccggt atctgcgcgc
     9061 ccacgcactg cattttccag ctcgatcggc gaaaccgtgg aggttcccag tttgccgacc
     9121 accacgccag ccgccgcatt ggcaaagaag caggcttctt ccagcgaatt acccgctgcc
     9181 agcgttgccg ccaggacgcc aatcaccgtg tcgcccgcac cggtaacgtc atacacttcc
     9241 tgcgcctggg ttggcatatg cagcggcgct ttacccggtt gcagcagcga cataccctgt
     9301 tcggaacggg tcactaacag agccgagagt tcgtaatcgg caatcagttt catgccgcgc
     9361 tcaacaatct cttcttcggt cttacattta ccgacaacag cttcaaattc cgagagattc
     9421 ggcgttaaca gcgtagcgcc gcggtagcgc tcaaaatcgg taccttttgg atcaatcagc
     9481 accggaacac ccgctttacg cgccagttgg atcatctgct gtacgcttgc cagcgcacct
     9541 ttggcgtagt cagaaagcac cagcgcgcca atcgaactca gcgcctgatt aatccgctcg
     9601 tgcagcggct gcggatcaac accttcgaaa ccttcttcaa aatccagacg gatcagctgt
     9661 tggttgcggg aaagtacccg taatttggta atggtcggat gcgtcggtac agaaacgaag
     9721 tcgcatttga cgttgacgtc ggccagagat ttactcagcg cgcgcgctgc atcgtcaatg
     9781 cccgtcaacc cgaccaggcg tgcattagca ccgagagaag cgatattcat cgccacgtta
     9841 gccgcgccgc ccggacgttc ttcgatggta ttcactttaa ccacgggcac cggcgcttcc
     9901 ggcgagatac gactggtggg gccgtaccag taacgatcca gcatcacatc accaaccacc
     9961 atcactcctg cacgttcaaa ctctggcagc gttactttca ttcctgtctc ctgagagatt
    10021 caaaatttgc gcgcgataat accatacttc attcttccac cagccacttc tgccagcttg
    10081 cccgcaccag ttcacgctct gcggtgaagc aatcctccga cacatggccc ggcaattcct
    10141 gtaatgccag atgatgaagt tcatcgcgaa gcgtagtgta agcacgggtc agcgccatcg
    10201 cttcctgctc ttccataatg tcgttttgcg ccagtagttc cagaatacgc acgttgtctg
    10261 accagcgcgt taacttcggt ttttcatgag cgtagcgcaa caccagatat tgggtaataa
    10321 attcgatatc ggtaattccc ccttcatcag ctttgatatc aaagcgatcg cgatgtttat
    10381 tgccgagatg agcgcgcatt ttctcgcgca tttcccgcac ttccgtttgc agagttttac
    10441 cttcacgcgg cagcgtcata atctcgcgac gcactgcgtc aaagtgcgcg gtgagctgcg
    10501 gatcgccgta cactacacgc gcacgcacca gcgcctgatg ttcccacgtc caggcctcgt
    10561 ttttctgata atcggcaaat gcttctgcgg atgtcaccag cattcccgcc gccccggacg
    10621 gacgcagtcg agcatccact tcatacaaaa tgccggaaga ggtacgcgta ctgaacagat
    10681 gcataatgcg ttgcgccaga cgcagataaa actgccgccc gtcgatttcc cgctcaccgt
    10741 cagtcatcgc atccattggg caatcatgga ggaagataag gtcaagatcg gaactgtagc
    10801 ctaactccca gccgcccagc ttgccgtagc cgaccaccgc aaaaccacgc ccttcgcgtt
    10861 cgttcaggtg attcggctta ccgtagcggg caaccatttg aacccacgcc tgctgaacga
    10921 cggcatctat catggcttcc gccagccagg ttaagtgatc gctcactttc atcaccggta
    10981 gcgtaccggc gatatccgct gcggcgatgc gtaacagctg cgcctgtttg aactgacgca
    11041 gcgcctcaag ctgttgctct tcgtcatctt ccggcacgcg cagcaaatac tggcgcaact
    11101 catcgcggta ggcatcggtc gccgtcggct ggtaaagggt gtttggatcg agcaattcat
    11161 ccagcaataa tggataacgc gccagctggc tggcaatcat cggcgacgcg gcacacagag
    11221 aaatcaaatg tttaagcgcc gcggggaatt cactgagcaa ttctaaatag gtggtgcggg
    11281 taacaatccc caccagcaag gcggtaatgc gcgacagcgt aacggcagcg tcttcacgcg
    11341 cacagacatc acttagcaga tgcggcatca gatggtcgag cacctgacgt cctcgcggcc
    11401 cgatggtgcg cttatccagc tctttgcgga aatcggcaat cagcgttagc acctgtttgc
    11461 gatcatcctc gctaagatgc gccagcactg gcgtagtgtc atcttcctgc aacgcatcct
    11521 gccacagctc acgccactgt tccgacagcg actcttcctg agtttcactt tcgtcgtcgc
    11581 caatcaattc attaaacacc cggcgcacat tggtcatatg tgcggtcagc gccccggtca
    11641 attgcggcca gtcagcaaag tccatcgccc acgccagccg cgcacgatta agctcatcag
    11701 aaggaagcgt ctgggtttgt tcgtcgttaa tgctttgcag caggttttcc agacgccgca
    11761 ggaacagata cgccactcgc aattgttcag catcgttttc agaaagcaga tgcagctcgg
    11821 caatggcgct gagcgttggc agtaaagagc gcgattgcag cgacggttcg cgtccgccgc
    11881 gaatgagctg gaacacctga acgataaatt caatttcgcg aatgccgcct gcgccgagtt
    11941 tgatattgtc ggtcaaacca cgtcgacgca cttcacgggc aatcatccct ttcatgttgc
    12001 gcagcgactg aatcacgctg aaatcgatgt aacgacggaa aacaaacggg cgcagcatcg
    12061 cacgcaactc gttagcatag acgccttcgc tatcgcccat aatccgcgcc ttgaccatcg
    12121 cgtaacgctc ccagtcgcgc ccctgctcct ggtaataatc ttccaacgcg gcaaagctca
    12181 gcaccagcgg gccactttcg ccaaacggac gcagccgcat atccacgcga tagacgaagc
    12241 catcctgcgt tggttgatcc agcactttaa tcagccgctg ccccatgcgg gtaaaaaact
    12301 gcgcgttatc cagttcccgg cgtccaccct gcgtacaacc atgttccggc caggcaaaaa
    12361 tcagatcgat atcagaggag aaattcagtt ccccgccgcc cagcttaccc atgcctaaaa
    12421 tcagcagcgg ttgcgcttcg ccctgcgcat tgcacggcgt tccccactcg cggcagcagg
    12481 cgtcatatag ccagtcacgc gccgcaacaa tcagcgtctc cgccagatag ctgagctgct
    12541 gcaatatgct ctcttcagta accagtgcca gcgtttgcgc ccaggcgata cgtaccatga
    12601 tgcgccgacg gaacagccgc agctcgcgca ttaacccggc ttcgtcactc acattactga
    12661 gcgcctcctg caaccatgca acgtaatgct gccattcgtc ggcctgcggc ggttggcttt
    12721 ccagttccgt cagccactct ggatgcgcac ttatgctgtc ctgcacaaaa tcactgaaag
    12781 taagtactga cttcgcctgt gcactaagtg attcctcggc taaaggctct ggcagccgct
    12841 caacaacggt ctgccagtac tgctgtaacg gtgaagagag cggcttcata agcgatttta
    12901 tccttgcctg attaacgttt tccgctgtgc aaccagaacg gttcctgatt gtttgcctca
    12961 ttacggaaat gttcaatttc gatgcgttgc ccggtcgcaa tagcgtgatg cagcccctgc
    13021 cagttctcca gccaggcttg cgagacgaca ggatcatagt aacccgccag caacagtatt
    13081 gagtcaatat cacgcgtcag gcgtggcaac tggtcacggt agcgatcgcc taacggctgg
    13141 cagaaaacgc ttttcagttc agcggcatgg cgggaaagat ggatatcggc aaagcgtttg
    13201 aaggagtcgc tgattttgcc ctgcgctttg gcatctaaaa atggctgcca tgctttgctt
    13261 accaaccatt cggtcaacgc cagcttcgcc attgccgttt cggtagagta gaccgccgtc
    13321 acggcagaca ccgcagaagc aatggtcgcc tcgcattgag tcagcagatc acgtaagtga
    13381 gtgttcgctt tacgcggcac aataccaccg aacagcatca gcgtatgacg gaccaggcta
    13441 atggctgcca gcacctgttc tttcgccgca tcgttgccgc gtacccacag ttcttcatga
    13501 tactgccatt gcgctaacgc cagctcgagc gccgcttcca gcccctgttc cacatcggct
    13561 tttgccgcaa catgcaaaat ggtggtcggt ttgatttcac gcgccggatt gccctgcgcc
    13621 agatgatagc cacgcgccgc tttgctcagg ctgccctggc gtaatccggt ttgcgatacc
    13681 agttggttcg ccagtttcag caccgcgcgc gtgtcgccgc taagcagttc cagttccagc
    13741 tcacagatag gttcagcaaa ttcacccgct ttcacttccc cctggtcgag ggcgatttca
    13801 atttggctac catcgaccgc caccagccat ttttcgcgat aaaaatcggt gctgaacagc
    13861 ggctgcacgc gggaggcgag atcggcgggc aattcgccgt tcggccagac ttccgtcggt
    13921 aactgcgcca ggtcgagcgt cggttcgctc aacgccacgt tatattccgg gcgctgatgt
    13981 aagccgcctg tcactcttcc tgcaactttc atggtcatct catagcgacc attttcgcca
    14041 cgaatacgta agcccatatc gtgcccacgc agccagttat ccggcgtttc gtagtaaata
    14101 ttcagcaact gcacggggtc atggtgctcg ccgcccagcg tattgagatg gtcacgcaac
    14161 gcctcaacgg cactgtgatt aacaataaac tttaattcga tttcctgagc catggccttt
    14221 acttatcggt tatgtcacat aagtgacgat gaacggcgaa cttaatgcga tcttttttgt
    14281 cagtagatag tattttgcgc caaattgcca tgcaacgggc aatttgacgg gcgtaaaagt
    14341 ttgaagcagt ggcaagacct gacacagagg attccgattg atgtcgaatg ctttgcgtag
    14401 cgacactgat gccactacta tcgttccact ttccatgaca ataacgacag cctgatgcca
    14461 aaattacgcc tgatcggatt aactttactc gcacttagcg cgactgccgt ctcacacgct
    14521 gaagaaacgc gctatgtttc cgacgaactg aatacctggg tccgcagcgg tccgggagat
    14581 cattatcgcc tcgtgggcac ggttaacgcc ggcgaggaag tgaccttatt acaaactgac
    14641 gccaacacca attatgccca ggtgaaagac agctctggcc gtaccgcctg gatcccgttg
    14701 aaacaactta gcactgagcc aagcctgcgc tcccgtgtgc cagatctgga aaatcaggtc
    14761 aaaaccctga ccgataaact caccaatatc gataacacct ggaatcagcg caccgcagaa
    14821 atgcagcaaa aagtggcgca gagcgacagc gtgatcaacg ggttaaaaga agaaaatcag
    14881 aaactgaaaa acgagctgat tgtcgcgcag aaaaaggtcg atgccgccag cgtacagctg
    14941 gatgacaaac agcgcaccat catcatgcaa tggtttatgt atggcggtgg cgtgctgggg
    15001 cttggcttgc tgctcggtct ggtactgccg cacctgatcc caagccgcaa acgcaaagat
    15061 cgctggatga actaaatcgc cttcttcgtt gcactgtctt attattagga attgtgtatt
    15121 tgacgaaggg atgatggcgt gaagatttat ctggtcggtg gtgctgttcg ggatgcattg
    15181 ttagggctac cggtcaaaga cagagattgg gtggtggtcg gcagtacgcc acaggagatg
    15241 ctcgacgcgg gctaccagca ggtaggccgc gattttcctg tgtttctgca tccgcaaacg
    15301 catgaagagt atgcgctggc acgtaccgaa cggaaatccg gttccggtta caccggtttt
    15361 acttgctatg ccgcaccgga tgtcacgctg gaagatgatc ttaagcgtcg cgatctgacc
    15421 attaatgcgc tggcccagga cgataacggc gagattatcg acccgtacaa cggtctgggc
    15481 gatctgcaaa atcgtctgtt gcgccatgtt tcccccgctt ttggcgaaga tccgttacgc
    15541 gtattgcgcg tggcgcgttt tgctgcgcgt tatgcccacc tcggttttcg tattgccgat
    15601 gaaactctgg cgttgatgcg cgagatgacc catgcgggtg aactggaaca cctgacgcct
    15661 gaacgggtat ggaaagagac ggaaagcgcc ctcaccaccc gcaatccaca ggtgttcttc
    15721 caggtactgc gcgattgcgg cgcactgcgc gttttattcc cggaaattga cgcactgttt
    15781 ggcgttccgg cccctgccaa gtggcatccg gaaatcgata cgggtattca taccttaatg
    15841 acgctctcaa tggcggcgat gctgagtccg caggtcgatg tccgtttcgc aactttatgc
    15901 cacgatctcg gtaaagggct gacgccgcca gaactctggc cgcgtcatca tggtcatggc
    15961 ccggcgggtg ttaagttagt ggaacaacta tgccagcgtc tgcgcgtgcc aaatgaaatt
    16021 cgcgatttag ccagactggt ggctgagttt cacgatctca tccacacctt cccaatgctg
    16081 aacccaaaaa ccatcgtcaa attatttgat tccatcgatg cctggcgtaa accgcagcgt
    16141 gtcgagcaac tggcgctgac cagcgaggct gacgtgcgcg gcagaaccgg ttttgaatca
    16201 gcggactacc cgcaaggccg ctggttgcgc gaagcctggg aagtggcgca gtcagtgccg
    16261 acaaaagccg tcgttgaagc gggatttaaa ggtgtggaga ttcgcgagga gctgacccga
    16321 cggcggattg cggcggtagc cagctggaag gaacaacgtt gcccaaagcc tgaatgagta
    16381 ttgggcattt tgccggatgc ggcgtgaacg ccttattcgg cctacgcaat cgttgacaac
    16441 gccaagcatc cgacactatt cctcaattaa aagaacacga catacaccgc agccgccaca
    16501 ataaagcgat aaatggcgaa cgggataaac gaaatgcgct taatcaattg caggaaggtt
    16561 ttaatcgcta tcagcgccac cacaaaagcg gtgataaacc caacggcaaa catcgggata
    16621 tcgccgcttg tcaggaagcc ccagcttttg tagagatcga gcgccgttgc gcccatcatc
    16681 atcggcaccg ccagcaggaa cgaaaactcg gaagcagcgt aacggctcac ccccatcagc
    16741 atcccacctg aaatggtcgc cccggaacgg gagaaacccg gccacagcgc cagacactgg
    16801 aaacagccaa tcataaatgc ctgacgatag gtcatatcat caagacccgg cgcacgcggc
    16861 tctttcggct tcaggcattc ggcggcaatc agcaacaaac cgccaacgac cagcgcatac
    16921 atcacattta tcgggttaaa caatgactta atcgtgtcgt ggaacaacag ccccaatacc
    16981 accgccggaa tcatccccag caaaatgtgg atcagcgtta aacgaccttt gctttcacct
    17041 tcgtgctgca acgggcggcc aaagtggatg ccaatcaggc caaacagacg ccgccagaac
    17101 atcactacta ccgccagaat tgatcctaac tggatcacaa cttcaaaggt tttcgccgtg
    17161 tcgccctcaa accccaacaa gtgaccgaca ataatcatat ggcccgtgct ggataccggc
    17221 agaaattctg tcaatccttc gaccacaccc aatattgccg ctatcagcag cgagtgcata
    17281 tcgctcatca ataaacccct aaattattaa aatgtaccgc ttgtccgaac tactgcgtat
    17341 gaccaggtta taaccgtttg gtttaacagc tgtaaaatta attattttct ttcagattat
    17401 tgccacgctc aatgattacg ccaacattcg ccgcccgcgc cactgcgcct ggcttgctga
    17461 gtttgatacg cacccacggc gagttgaagc gtgctaacag cagctccgcc acctcttcag
    17521 ccacgcgttc caccagcgca aaacgcgccc cctcgacgtg gctgaccacc gtttctgcaa
    17581 tgtcagcgta actgaggcaa tccgccacat catcactttt cgccgcttta cggttatccc
    17641 atgccatttc gatatcgaac actaacttct gttcgatggt ctgttcccag tcgtaaacac
    17701 caatagtggt gattaccgaa agttgctcta taaatacaat atccatcacg tcctgcctgc
    17761 tttttggcta accggatacc acttccggcg aaatgtgcgt attatccaca gattaatcgt
    17821 tgaacacgaa ttttcaaaac ggaacagctt atgagtgcaa tcgcgcctgg aatgatcctc
    17881 atcgcgtacc tctgcggctc catttccagt gccattctgg tttgccgctt gtgtgggctg
    17941 cccgatccgc gaaccagcgg ctccggcaat ccaggcgcaa ccaatgtgtt acgtatcggt
    18001 ggcaagggag cagccgtagc agtactgatt ttcgacgttc tgaaaggaat gttgcccgtc
    18061 tggggcgcgt atgaattagg tgtcagcccc ttctggctag gcttaattgc catcgccgcc
    18121 tgtcttggac acatctggcc cgttttcttc ggatttaaag gaggaaaagg cgttgctacc
    18181 gcttttggtg ccatcgcacc cattggctgg gatctcaccg gagtaatggc gggaacctgg
    18241 ttactgaccg tgctattgag cggatactcg tcgctgggag cgattgtcag tgcactgatt
    18301 gctccgtttt atgtctggtg gtttaagcca caattcacct tcccggtttc gatgctctct
    18361 tgcctgatcc tgctgcgtca tcatgacaac atccagcgtc tgtggcgtcg tcaggagaca
    18421 aaaatctgga cgaaattcaa aagaaagcgc gaaaaggatc ccgagtgatt tctggtggat
    18481 ctacatgacc tgatagcctt catcgggctt gcccagccgt tgctggcacc atgccgccag
    18541 aaattccacg cagacacgta atttcatgct gcgatagagc ggctcccggt aaacagccca
    18601 gatattggcg ctttgtgcat actccggcaa tacttgcacc agtttgccac tctccagaaa
    18661 cggcaacaca tcccactcgg aacgcagcat aatccctttg ccctccagcg cccattgcaa
    18721 cactatctcg ccgctattgg aggaaagatg cccgctaacc ttcaccgatt ttttctcctg
    18781 accgttcccc aactcccata ttccgtgggt catatcgcgc tctttggtca ccagacagtc
    18841 atgacgactt aattcctgta atgattgtgg ctgcggatat ttttgcagat actcaggtgc
    18901 tgcgcataat attcttttat ttttcgttaa cagatgcgca atataataat caggaatttc
    18961 gtcattaata cgaatatcca gatcaatatt atcctgcact aaatcaattt gccgatcgaa
    19021 caattcaaaa tgcacctgta actcaggata attgcgcata agttcggtaa tggctggcgc
    19081 aatatggctg cgcccaaaac caaaactaca gccaatacga atcatcccct ccgggcgcgt
    19141 tttgatttgc gtgacgtcat cgaccagtcg ctgatattgg gtgaggattt ccagcgcatg
    19201 ttcgtaacaa cgttggccac tttcagtcag cgccacgccg cgagcggagc ggttcagcag
    19261 cgttgttgcc agcgtatttt caaggatttg gatccgcttg gtgacaaatg caggcgtctg
    19321 cccaagcgtc gccgctgcgg cgctaaaact cccgctgtgg acgatttcta ccagtacctg
    19381 caagtctttg gctaaaggcc agctattcag catcgttaat tatccgcagt tgtgataagc
    19441 gcagtgtatt tcgcaaaaca ttgccattcc ctgctctggc gcaatattca ttcttatttt
    19501 cgcgttgatt cacgaactgt taattacata ttcacggtgg caaaaaatat aaaaccacat
    19561 ttttagtggt agtttgtggc ggtgaatttt tccagacaaa tacaaaaact ggagttgcca
    19621 tgatgagcga aagtaataag caacaggcag tgaataagtt gacagagatt gtcgctaact
    19681 ttaccgccat gatttctacc cgaatgcctg atgacgtggt ggataaacta aaacagctaa
    19741 aggatgccga aacgtcgtcg atggggaaaa ttatctacca tacgatgttc gacaacatgc
    19801 aaaaagcgat tgacctgaat cgtcctgcct gtcaggacac cggggagatt atgttcttcg
    19861 ttaaagtcgg ttcccgcttc ccactgcttg gcgagctgca aagcatactc aaacaagccg
    19921 tggaagaggc aaccgtcaaa gcgccactac gtcacaatgc ggtagaaatt tttgacgaag
    19981 taaacaccgg caaaaatacc ggtagcggcg taccgtgggt cacctgggac atcatccccg
    20041 acaatgacga tgcggaaatc gaagtttaca tggcaggcgg cggctgcacg ctacctggcc
    20101 gctcgaaagt gttaatgccg tcagaaggct acgaaggcgt ggtgaaattc gtcttcgaaa
    20161 atatctccac cctcgccgta aacgcctgtc caccggtact ggtgggcgtg ggcatcgcca
    20221 cctcggtgga aaccgccgcc gtactctcgc gtaaagccat tttgcgcccg attggcagcc
    20281 gccatcccaa tccaaaagcg gcagaactgg agctacgcct ggaagaagga ctcaaccgtc
    20341 tggggattgg tccacaaggg ctgaccggca acagttcagt gatgggcgta catatcgaat
    20401 ctgccgcccg ccatccgtca accatcggcg ttgctgtctc taccggctgc tgggcgcatc
    20461 gtcgcggcac gctgctggtt catgccgatc tcacctttga aaatctgtct cacacccgga
    20521 gcgcgttatg aaaaagatcc tgacaacccc gatcaaagct gaagatctgc aagatattcg
    20581 cgtcggcgat gtgatctacc tgaccggtac gctggtgacc tgccgcgacg tttgtcaccg
    20641 ccgtttgatc gaactgaaac gtccgatccc ttacgatctc aacggcaaag cgattttcca
    20701 cgctggcccc atcgtgcgca aaaacggcga caaatgggag atggtctccg tcggcccgac
    20761 aaccagtatg cgtatggaaa gttttgaacg tgaatttatt gagcagaccg gcgtgaaact
    20821 ggtggttggc aaaggtggta tggggccgct gaccgaagaa ggctgccaga aattcaaggc
    20881 gctacatgtg attttcccgg caggctgcgc ggtgctggcg gcaacccagg tggaagagat
    20941 tgaagaagtg cactggacag agctcggaat gccggagtca ctgtgggtct gccgggtcaa
    21001 agagttcggc ccgctgattg tctctattga tacccacggc aacaacctga tagccgaaaa
    21061 caaaaagctg ttcgccgaac gccgcgatcc catcgtggaa gagatctgcg agcacgtcca
    21121 ttacatcaaa taaccctccc ggagaggctc acccctctcc tttttcgcag gcataacacg
    21181 atgaaacctt ccactgaatg gtggcgatat cttgcgccgc tggcggtcat cgccattatt
    21241 gctctacttc cggttcccgc agggctggag aaccatacct ggctctactt tgccgttttt
    21301 actggcgtga tcgttggact gatcctcgaa cccgtgccgg gtgccgtggt ggcgatggtg
    21361 ggtatatcca tcatcgccat actctctccc tggctgctgt tcagcccgga gcagctcgct
    21421 cagccaggct ttaaattcac tgcaaaatcc ctctcgtggg ccgtttccgg tttttctaat
    21481 tcggttatct ggctgatttt cgccgccttt atgtttggca caggctatga aaaaaccggg
    21541 cttggacgcc gcattgcgct gattctggtg aaaaagatgg gacatcgcac gctgtttctc
    21601 ggctatgcgg tgatgttctc cgagctgatc ctcgcacctg taacaccgtc caactcggcg
    21661 cgtggtgcgg ggattatcta tcccatcatc cgtaacctgc caccgctcta tcaatcacaa
    21721 ccaaacgaca gcagttcgcg cagcattggc tcgtacatca tgtggatggg gattgttgcc
    21781 gactgcgtga ccagcgccat tttcttgacg gcgatggcac caaacttgct gttaattgga
    21841 ctgatgaaaa gcgcatctca cgccacgctg agttggggcg actggttcct cgggatgttg
    21901 ccgctcagca ttttactggt tctgctggtt ccctggctgg cttacgtgct gtacccgccg
    21961 gtactgaagt ctggcgatca ggtgccgcgc tgggcagaga cggaactgca ggcaatgggc
    22021 ccgctctgtt cgcgtgaaaa acggatgctg gggctgatgg taggcgcgct ggtgctgtgg
    22081 attttcggcg gtgattatat cgatgccgcg atggtcggtt acagcgtggt ggcactgatg
    22141 ctgcttctgc gcattatcag ttgggacgac attgtcagta ataaagcggc gtggaacgtt
    22201 ttcttctggc tggcctcgct tatcaccctc gccaccggac tcaacaacac cggttttatt
    22261 agctggtttg gcaaactgtt agcaggcagc ttaagcggtt attcgccaac gatggtgatg
    22321 gtggcgttga ttgtggtgtt ttatctactg cgctactttt tcgccagcgc cacggcgtat
    22381 acctccgctc tcgcaccgat gatgattgcc gctgcgctgg cgatgccgga aatcccgctg
    22441 ccggtgttct gcctgatggt tggtgcggca attggtctgg ggagcattct tacaccatac
    22501 gccaccggcc ccagtccgat ttactacggc agtggttatc tgccaacggc ggattactgg
    22561 cgactggggg cgatttttgg gctgatattc ctcgtattgc tggtgattac cggcttactg
    22621 tggatgcccg tggtgttgct ttaagtgagg acggtgccgg aaattgctct ccggcaccca
    22681 atagttttac gcagccggta actccgccag cggccagcgc ggacgcacgc taacgccgag
    22741 atccgccgtc gcgcctgctt taaaccgcac cattccggca taggcgatca tcgcgccgtt
    22801 atcagtacaa aattccggac gcgcgtagaa cacttcgccg cggcgttttt tcatcatttc
    22861 agccagcttc gcccgtaacg tacggttagc actcacgccg cccgccatga ccagtcgctt
    22921 aaagcccgtc tgatccagcg cccgcttgca cttaatcatc agcgtatcga ccaccgcatc
    22981 ttcaaaggcg cgggcgatat cagcacgcgt ctggtcgtcg gtgccgttgt cacgaatggt
    23041 atttgccgcg aaggttttca ggccggagaa gctgaaatcc agccccggac ggtcggtcat
    23101 cggacgcggg aagacaaagc gcccggcagt accctgagcc gccattttcg acagtaacgg
    23161 cccgccagga taatccagcc ccagcagctt cgcggtttta tcaaacgctt ccccggcggc
    23221 atcatcgata gactcgccga gcagctcgta ctgaccaatg ccagtcacgc tgattaactg
    23281 cgtatgaccg ccggaaacaa gcagcgcaac aaacggaaat tccggcgggt tatcttccag
    23341 catcggcgct aacagatgcc cttccatatg gtgtacaggg atcgccggaa cgtcccaggc
    23401 aaacgccaga gaacgcccca cggtcgcgcc aaccagtagc gcgccgacta atccagggcc
    23461 tgcggtatag gccacagcat caatgtcttt tgccgttaaa ccagactcct ttagcgccgc
    23521 ctggatcaac ggtacggttt tacgcacatg atcgcgggag gccagttcag gcacgacgcc
    23581 gccgtagtca gcgtgcaatt tcacctgact atacaattgg ttggctaaca aacctttttc
    23641 atcgtcgtaa atggcgatgc cggtttcatc gcaggaagtt tcaataccca gtacacgcat
    23701 gacttgtttt acctcgcttt attaccgcgc agtgtaggac caatgcgggt tgatgtaaaa
    23761 ctttgttcgc ccctggagaa agcctcgtgt atactcctca cccttataaa agtccctttc
    23821 aaaaaaggcc gcggtgcttt acaaagcagc agcaattgca gtaaaattcc gcaccatttt
    23881 gaaataagct ggcgttgatg ccagcggcaa accgaattaa tcaaaggtga gaggcacatg
    23941 ccggtaatta aagtacgtga aaacgagccg ttcgacgtag ctctgcgtcg cttcaagcgt
    24001 tcctgcgaaa aagcaggtgt tctggcggaa gttcgtcgtc gtgagttcta tgaaaaaccg
    24061 actaccgaac gtaagcgcgc taaagcttct gcagtgaaac gtcacgcgaa gaaactggct
    24121 cgcgaaaacg cacgccgcac tcgtctgtac taattccccg agagcgttgc tctccgatca
    24181 gaccgagttg tagttgtaag gccgtgcttc cgaaaggaat gcgcggctta ttttcgttta
    24241 tgaattgcta aaaatcgggg cctatggctg gacgaatccc acgcgtattc attaatgatc
    24301 tgctggcacg cactgacatc gtcgatctga tcgatgcccg tgtgaagctg aaaaagcagg
    24361 gcaagaattt ccacgcgtgt tgtccattcc acaacgagaa aaccccgtcc ttcaccgtta
    24421 acggtgagaa acagttttac cactgctttg gatgtggcgc gcacggcaac gcgatcgact
    24481 tcctgatgaa ctacgacaag ctcgagttcg tcgaaacggt cgaagagctg gcagcaatgc
    24541 acaatcttga agtgccattt gaagcaggca gcggccccag ccagatcgag cgccatcaga
    24601 ggcaaacgct ttatcagttg atggacggtc tgaatacgtt ttaccaacaa tctttacaac
    24661 aacctgttgc cacgtctgcg cgccagtatc tggaaaaacg cggattaagc cacgaggtta
    24721 tcgctcgctt tgcgattggt tttgcgcccc ccggctggga caacgtcctg aagcggtttg
    24781 gcggcaatcc agaaaatcgc cagtcattga ttgatgcggg gatgttggtc actaacgatc
    24841 agggacgcag ttacgatcgt ttccgcgagc gggtgatgtt ccccattcgc gataaacgcg
    24901 gtcgggtgat tggttttggc gggcgcgtgc tgggcaacga tacccccaaa tacctgaact
    24961 cgccggaaac agacattttc cataaaggcc gccagcttta cggtctttat gaagcgcagc
    25021 aggataacgc tgaacccaat cgtctgcttg tggtcgaagg ctatatggac gtggtggcgc
    25081 tggcgcaata cggcattaat tacgccgttg cgtcgttagg tacgtcaacc accgccgatc
    25141 acatacaact gttgttccgc gcgaccaaca atgtcatttg ctgttatgac ggcgaccgtg
    25201 caggccgcga tgccgcctgg cgagcgctgg aaacggcgct gccttacatg acagacggcc
    25261 gtcagctacg ctttatgttt ttgcctgatg gcgaagaccc tgacacgctg gtacgaaaag
    25321 aaggtaaaga agcgtttgaa gcgcggatgg agcaggcgat gccactctcc gcatttctgt
    25381 ttaacagtct gatgccgcaa gttgatctga gtacccctga cgggcgcgca cgtttgagta
    25441 cgctggcact accattgata tcgcaagtgc cgggcgaaac gctgcgaata tatcttcgtc
    25501 aggaattagg caacaaatta ggcatacttg atgacagcca gcttgaacga ttaatgccaa
    25561 aagcggcaga gagcggcgtt tctcgccctg ttccgcagct aaaacgcacg accatgcgta
    25621 tacttatagg gttgctggtg caaaatccag aattagcgac gttggtcccg ccgcttgaga
    25681 atctggatga aaataagctc cctggacttg gcttattcag agaactggtc aacacttgtc
    25741 tctcccagcc aggtctgacc accgggcaac ttttagagca ctatcgtggt acaaataatg
    25801 ctgccaccct tgaaaaactg tcgatgtggg acgatatagc agataagaat attgctgagc
    25861 aaaccttcac cgactcactc aaccatatgt ttgattcgct gcttgaactg cgccaggaag
    25921 agttaatcgc tcgtgagcgc acgcatggtt taagcaacga agaacgcctg gagctctgga
    25981 cattaaacca ggagctggcg aaaaagtgat ttaacggctt aagtgccgaa gagcgatcgg
    26041 gaagcccccg acagccgcac tgagaggcag cggcaaatat ataagtacgc cctcgtaatt
    26101 atcgttggcg gtaaacaacc gttggatttc agcgttaacg gctgaaggac atcgggtcaa
    26161 tcgcccaaca ccaacctcat gaaataagtg tggataccgt cttatggagc aaaacccgca
    26221 gtcacagctg aaacttcttg tcacccgtgg taaggagcaa ggctatctga cctatgccga
    26281 ggtcaatgac catctgccgg aagatatcgt cgattcagat cagatcgaag acatcatcca
    26341 aatgatcaac gacatgggca ttcaggtgat ggaagaagca ccggatgccg atgatctgat
    26401 gctggctgaa aacaccgcgg acgaagatgc tgccgaagcc gccgcgcagg tgctttccag
    26461 cgtggaatct gaaatcgggc gcacgactga cccggtacgc atgtacatgc gtgaaatggg
    26521 caccgttgaa ctgttgaccc gcgaaggcga aattgacatc gctaagcgta ttgaagacgg
    26581 gatcaaccag gttcaatgct ccgttgctga atatccggaa gcgatcacct atctgctgga
    26641 acagtacgat cgtgttgaag cagaagaagc gcgtctgtcc gatctgatca ccggctttgt
    26701 tgacccgaac gcagaagaag atctggcacc taccgccact cacgtcggtt ctgagctttc
    26761 ccaggaagat ctggacgatg acgaagatga agacgaagaa gatggcgatg acgacagcgc
    26821 cgatgatgac aacagcatcg acccggaact ggctcgcgaa aaatttgcgg aactacgcgc
    26881 tcagtacgtt gtaacgcgtg acaccatcaa agcgaaaggt cgcagtcacg ctaccgctca
    26941 ggaagagatc ctgaaactgt ctgaagtatt caaacagttc cgcctggtgc cgaagcagtt
    27001 tgactacctg gtcaacagca tgcgcgtcat gatggaccgc gttcgtacgc aagaacgtct
    27061 gatcatgaag ctctgcgttg agcagtgcaa aatgccgaag aaaaacttca ttaccctgtt
    27121 taccggcaac gaaaccagcg atacctggtt caacgcggca attgcgatga acaagccgtg
    27181 gtcggaaaaa ctgcacgatg tctctgaaga agtgcatcgc gccctgcaaa aactgcagca
    27241 gattgaagaa gaaaccggcc tgaccatcga gcaggttaaa gatatcaacc gtcgtatgtc
    27301 catcggtgaa gcgaaagccc gccgtgcgaa gaaagagatg gttgaagcga acttacgtct
    27361 ggttatttct atcgctaaga aatacaccaa ccgtggcttg cagttccttg acctgattca
    27421 ggaaggcaac atcggtctga tgaaagcggt tgataaattc gaataccgcc gtggttacaa
    27481 gttctccacc tacgcaacct ggtggatccg tcaggcgatc acccgctcta tcgcggatca
    27541 ggcgcgcacc atccgtattc cggtgcatat gattgagacc atcaacaagc tcaaccgtat
    27601 ttctcgccag atgctgcaag agatgggccg tgaaccgacg ccggaagaac tggctgaacg
    27661 tatgctgatg ccggaagaca agatccgcaa agtgctgaag atcgccaaag agccaatctc
    27721 catggaaacg ccgatcggtg atgatgaaga ttcgcatctg ggggatttca tcgaggatac
    27781 caccctcgag ctgccgctgg attctgcgac caccgaaagc ctgcgtgcgg caacgcacga
    27841 cgtgctggct ggcctgaccg cgcgtgaagc aaaagttctg cgtatgcgtt tcggtatcga
    27901 tatgaacacc gaccacacgc tggaagaagt gggtaaacag ttcgacgtta cccgcgaacg
    27961 tatccgtcag atcgaagcga aggcgctgcg caaactgcgt cacccgagcc gttctgaagt
    28021 gctgcgtagc ttcctagacg attaatcggt aggccggatc aggcgttacg ccgcacccgg
    28081 cactaggccc tctgcacaaa cgccaccttt tcggtggcgt tttttatcgc ccacgcacta
    28141 ccagcgcctg gtccagctcg cgatacgctt caaccagttt ctccagtgaa acgcgactta
    28201 aaccgctggg atttggcagc acccaaatct gcgtcgaacc aatggtgagc gtttgtttcc
    28261 cccactgtgc accgcgctgg ctgaatccct gttcatatgc ttgtttgccc agaatcgcca
    28321 acgcctgcgg ctgataatct tcaatttttt caatcagctt acgcccgcct gcgtgtagct
    28381 cctgctttga aacttcattg gcttgcaccg ttggacggtc taccagtttg gtgacgccac
    28441 aacgataatc cagcagatgc tgtgcctcct gcggcttcaa ctgacggtcg gtaaacccgg
    28501 cctgatatat caccttccag aagcgatttg ccggatgagc aaagggaaaa ccagtcccgg
    28561 cggatgaaag cccagggttg ataccgcaaa acacgacccg taaccctgga gccaaaatat
    28621 cctcaaccat atttactcct ttctaacatc accaggaaaa gtataacgga ttgaaaatac
    28681 attgtttata aaaacagcag gcgcgcggta atggctggat tgcgacacgg agttacttta
    28741 taatccgcta ccatggcccc ttagctcagt ggttagagca ggcgactcat aatcgcttgg
    28801 tcgctggttc aagtccagca ggggccacca gatatagcaa aggctgacga gaaatcgtca
    28861 gcctttttct ttttatatat cagttacttt gcgtgccagt aagccgctgc acgtacccgc
    28921 tgtgggtcat actgttccgc ttcaaagcgg cggcttaaat tcttaacgac tttaccttcg
    28981 ccggttatcc agatgaagta atcatcggca gggatttgca tctgcgccag acgcgcatct
    29041 accgcctgct catcatgtgc cagccattcg atattaaaac catcaaggtg cgcgagataa
    29101 tcctgacagg cgttatcccg cacgctaacc agcgcactaa cttgcggttt aacggcaagt
    29161 ttgctcaaca tttccaggcg gcggcgcaat gcgggcattc cggattcatc gcagacatac
    29221 agctgatacg cgtaatcttc cggcaccacc agcgaaccgc gcggacctgc caccgtaagt
    29281 ttatcgcccg gttgcgcctg catcgcccag ccgctggcga ccccaccgtc gtgaataaag
    29341 aaatcaatca ccagttcatg gcgtagttcg tcatacagcg gcgtatagtc acgcgacggt
    29401 gggcgtggtc cttccggcca gacgatgccc tcttccgtta ccgttggcgg cacaaagtga
    29461 gcgtcaggtt gaggaaagaa gagtttgctg tgatcgtcaa agccacgcga tgtaaaaccg
    29521 tccagcgcct cgccgccgag gacaatgcgc tgaaaaccgg cgctgatgcg ctcaacgcgt
    29581 aacacagtca gttcacggaa gcgcagatca ttgcgaacgc gctgcgggta gcggggggag
    29641 ttattcattg ttatcgcctt cgtgatggta attagatata tctaaataaa actcgcaaat
    29701 gataatgatt gttaatcatg ataaatgcaa gcgatttgta gaactgatat atctgtagtc
    29761 tgataagacg cgcaacctct tatca
//
LOCUS       DAJOWB010000048        29639 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJOWB010000048 DAJOWB010000000
VERSION     DAJOWB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.048"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(34..1863)
                     /gene="glmS"
                     /locus_tag="OHO85_002334"
     CDS             complement(34..1863)
                     /gene="glmS"
                     /locus_tag="OHO85_002334"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCQ2671698.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2025..3395)
                     /gene="glmU"
                     /locus_tag="OHO85_002335"
     CDS             complement(2025..3395)
                     /gene="glmU"
                     /locus_tag="OHO85_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCQ2671699.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3746..4165)
                     /gene="atpC"
                     /locus_tag="OHO85_002336"
     CDS             complement(3746..4165)
                     /gene="atpC"
                     /locus_tag="OHO85_002336"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCQ2671700.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4186..5568)
                     /gene="atpD"
                     /locus_tag="OHO85_002337"
     CDS             complement(4186..5568)
                     /gene="atpD"
                     /locus_tag="OHO85_002337"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCQ2671701.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5595..6458)
                     /gene="atpG"
                     /locus_tag="OHO85_002338"
     CDS             complement(5595..6458)
                     /gene="atpG"
                     /locus_tag="OHO85_002338"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCQ2671702.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6509..8050)
                     /gene="atpA"
                     /locus_tag="OHO85_002339"
     CDS             complement(6509..8050)
                     /gene="atpA"
                     /locus_tag="OHO85_002339"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCQ2671703.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8063..8596)
                     /gene="atpH"
                     /locus_tag="OHO85_002340"
     CDS             complement(8063..8596)
                     /gene="atpH"
                     /locus_tag="OHO85_002340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCQ2671704.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8611..9081)
                     /gene="atpF"
                     /locus_tag="OHO85_002341"
     CDS             complement(8611..9081)
                     /gene="atpF"
                     /locus_tag="OHO85_002341"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCQ2671705.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9143..9382)
                     /gene="atpE"
                     /locus_tag="OHO85_002342"
     CDS             complement(9143..9382)
                     /gene="atpE"
                     /locus_tag="OHO85_002342"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCQ2671706.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9429..10244)
                     /gene="atpB"
                     /locus_tag="OHO85_002343"
     CDS             complement(9429..10244)
                     /gene="atpB"
                     /locus_tag="OHO85_002343"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCQ2671707.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10253..10633)
                     /gene="atpI"
                     /locus_tag="OHO85_002344"
     CDS             complement(10253..10633)
                     /gene="atpI"
                     /locus_tag="OHO85_002344"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCQ2671708.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11250..11873)
                     /gene="rsmG"
                     /locus_tag="OHO85_002345"
     CDS             complement(11250..11873)
                     /gene="rsmG"
                     /locus_tag="OHO85_002345"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCQ2671709.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11937..13826)
                     /gene="mnmG"
                     /locus_tag="OHO85_002346"
     CDS             complement(11937..13826)
                     /gene="mnmG"
                     /locus_tag="OHO85_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCQ2671710.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14205..14648)
                     /gene="mioC"
                     /locus_tag="OHO85_002347"
     CDS             complement(14205..14648)
                     /gene="mioC"
                     /locus_tag="OHO85_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCQ2671711.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKDSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14738..15196)
                     /gene="asnC"
                     /locus_tag="OHO85_002348"
     CDS             complement(14738..15196)
                     /gene="asnC"
                     /locus_tag="OHO85_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCQ2671712.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15348..16340
                     /gene="asnA"
                     /locus_tag="OHO85_002349"
     CDS             15348..16340
                     /gene="asnA"
                     /locus_tag="OHO85_002349"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCQ2671713.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKVLPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16345..17796)
                     /gene="viaA"
                     /locus_tag="OHO85_002350"
     CDS             complement(16345..17796)
                     /gene="viaA"
                     /locus_tag="OHO85_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCQ2671714.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17790..19286)
                     /gene="ravA"
                     /locus_tag="OHO85_002351"
     CDS             complement(17790..19286)
                     /gene="ravA"
                     /locus_tag="OHO85_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCQ2671715.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19509..21377
                     /gene="kup"
                     /locus_tag="OHO85_002352"
     CDS             19509..21377
                     /gene="kup"
                     /locus_tag="OHO85_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCQ2671716.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21544..21963
                     /gene="rbsD"
                     /locus_tag="OHO85_002353"
     CDS             21544..21963
                     /gene="rbsD"
                     /locus_tag="OHO85_002353"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCQ2671717.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPISKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            21971..23476
                     /gene="rbsA"
                     /locus_tag="OHO85_002354"
     CDS             21971..23476
                     /gene="rbsA"
                     /locus_tag="OHO85_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HCQ2671718.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLGKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23481..24446
                     /gene="rbsC"
                     /locus_tag="OHO85_002355"
     CDS             23481..24446
                     /gene="rbsC"
                     /locus_tag="OHO85_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCQ2671719.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24471..25361
                     /gene="rbsB"
                     /locus_tag="OHO85_002356"
     CDS             24471..25361
                     /gene="rbsB"
                     /locus_tag="OHO85_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HCQ2671720.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25487..26416
                     /gene="rbsK"
                     /locus_tag="OHO85_002357"
     CDS             25487..26416
                     /gene="rbsK"
                     /locus_tag="OHO85_002357"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCQ2671721.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26420..27412
                     /gene="rbsR"
                     /locus_tag="OHO85_002358"
     CDS             26420..27412
                     /gene="rbsR"
                     /locus_tag="OHO85_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCQ2671722.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLSIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27378..28805)
                     /gene="mdtD"
                     /locus_tag="OHO85_002359"
     CDS             complement(27378..28805)
                     /gene="mdtD"
                     /locus_tag="OHO85_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCQ2671723.1"
                     /translation="MSDKKNRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(28828..29520)
                     /locus_tag="OHO85_002360"
     CDS             complement(28828..29520)
                     /locus_tag="OHO85_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCQ2671724.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7120 a         7604 c         7579 g         7336 t
ORIGIN      
        1 cggtcggcag ccctttttta tctatgatag tgattactca accgtaaccg attttgccag
       61 gttacgcggc tggtcaacgt cggtgccttt gatcagcgcg acatggtaag ccagcagctg
      121 cagcggaacg gtgtagaaga tcggtgcaat cacctcttcc acatgcggca tctcgatgat
      181 gtgcatgtta tcgctactta caaaacccgc atcctgatcg gcgaagacat acaactgacc
      241 gccacgcgcg cgaacttctt caatgttgga tttcagtttt tccaacagtt cgttgttcgg
      301 tgcaacgacg ataaccggca tatccgcgtc aatcagcgcc agcgggccgt gtttcagctc
      361 gcctgcagcg taggcttcag cgtgaatgta agagatctct ttcagcttca atgcgccttc
      421 cagcgcgatt gggtactgat cgccacggcc caggaacagc gcgtgatgtt tgtcagagaa
      481 atcttctgcc agcgcttcaa tgcgtttgtc ctgagacagc atctgctcaa tacggctcgg
      541 cagcgcctgc agaccatgca caatgtcatg ttcaatggag gcatccagac ctttcaggcg
      601 agacagcttc gccaccagca tcaacagcac agttaactga gtggtgaatg ctttagtgga
      661 tgccacgccg atttccgtac ccgcgttggt catcagcgcc agatcggatt cgcgcaccag
      721 agaagaaccc ggaacgttac agattgccag cgaaccaagg taacccagct ctttcgacag
      781 acgcaggccc gccagggtat ccgcggtttc gccagactgt gacaaggtga tcatcaggct
      841 gttacgacgc acggcagatt tgcgatagcg gaattcagag gcgatttcga cgtcgcacgg
      901 aatacctgcc agcgattcaa accagtaacg ggaaaccata ccggagttat aagaagtacc
      961 acaggcgagg atctgaatat gctcaacctt cgacagcagt tcgtcggcgt tcggtcccag
     1021 ctcgcttaaa tcaacctgac cgtggctgat gcgcccggta agggtgtttt tgatcgcgtt
     1081 cggctgttcg tagatctctt tctgcatgta gtgacggtaa atacctttat cgcccgcgtc
     1141 atattgcaga ttggattcga tatcctgacg ttttacttcc gcgccagttt tatcgaagat
     1201 gtttaccgaa cggcgagtaa tttccgcaat atcgccctct tcaaggaaga taaagcgacg
     1261 ggtcaccggc aacagcgcca gctggtcaga agcgataaag ttttcgccca tacccaggcc
     1321 aatcaccagc ggactaccag aacgtgccgc cagcagggta tccgggtgac gggagtccat
     1381 gatcactgta ccgtacgcac cacgcagttg cgggatagca cgcagaacag cctcacgcag
     1441 agtaccgccc tgtttcagct cccagttcac cagatgggca attacttcgg tgtctgtttc
     1501 agaaacgaag gtatagccac gcgcttttaa ctcttcgcgc agcggttcat ggttttcgat
     1561 gatgccgtta tgcaccacca caatgtgttc agaaacatgc ggatgcgcat tcacttctga
     1621 aggttcaccg tgggtcgccc agcgagtatg agcaataccc gtaccgccat gcagaggatg
     1681 ttcttccgct gcctgagcca gcatctggac tttaccgagg cgacgcaggc gggtcatatg
     1741 accttccgca tcaacaacgg ccagaccggc agagtcatat ccgcggtatt ccagacgacg
     1801 taaaccttca agaaggattt ctgctacatc acgttgcgcg atcgcgccaa caattccaca
     1861 catagttttt gattccgatt tatatcgttg tcggtcaacc tgtatgcccg ttttttcggg
     1921 cgccccgagc cttgtagaga gtggggttat ttttatagtt actgcttgtg ggagggagat
     1981 tatgttatct cctcatccca gatgaccgga ttacccggcc agaattactt tttctttacc
     2041 ggacgacgcc agccttcttt ctgagtctgc ggcacacggc tgatagctaa tgcattttcg
     2101 ccgacattac gcgtcacagt tgtacccgca gcaatggtcg cgcctttgcc tactgttacc
     2161 ggggccacca gctgagtgtc ggaaccgaca aacacatcgt cgccgataat ggtcttaaat
     2221 ttattcgcac catcgtagtt gcaggtaatg gttcccgcgc cgatgttaac gttatcgcca
     2281 atttccgcat cgcccaggta agtcagatga ccagctttcg agcctttacc cagacgcgct
     2341 tttttcatct caacaaagtt accgacgtgt gcaccttcca gcaactcagc accaggacgc
     2401 agacgggcaa acgggccaat agtacaggcc gctgccagat tcgcgtcttc cacaacggta
     2461 tacggactga tttcgcaatc atcgccaatc acgctgtttt taatcacgca accggtgccg
     2521 attttcacgc gatgaccgag agtcacgttg ccctcgatga taacgttagt atcaatttca
     2581 acatcgcgcc cgtgagtaag cgtaccgcgc agatcaaaac gcgccggatc gcgcagcata
     2641 acgcctgcta acagcagttt ttcagcctgt tcggactggt aaacgcgctc cagacgggag
     2701 agttgcaggc ggttattcac accttctact tcgcttaaac gttgcggatg aacggcgacg
     2761 atttcacgcc cttcctgata cgccagcgca ataatgtcgg tgatgtagta ttcgccctga
     2821 gcattattgt tggtcagctt cgccagccag cgtttcatat ctgcgccgtt agcgatcaga
     2881 atgccggtgt tgatctcctg aatctgacgc tgctcgtcgg tggcatcttt gtgctcaaca
     2941 atgccggtaa ctttgccatt ttcacgggtg atacgtccat aaccggtcgg atcatccagt
     3001 ttcaccgtca gcagaccaat gccaccctgc ggtttagcat cacgcaggcg ctggagtgtt
     3061 tcgacagaga tcagcggcac gtcgccgtag agcattaaaa tgtcttcatc atcggcaaag
     3121 aaaggtgcgg cctgctgcat tgcatgaccc gtacccagct gctctgcctg aagcacccag
     3181 ttaaggttgt cgtctttcag cgcctgttta agcagatcgc cgccgtgacc gtacaccagg
     3241 tgaacgtgcg ctgcgcctaa ttcattcgca gcatcaatga catgctgaac catcgctttc
     3301 ccggcaaggg tatgcagcac tttcggaaga tcggaataca tgcgcgtgcc tttgcctgcg
     3361 gcaaggatca ctacgctcat agcattattc aacatacgcg tcctgactgt aatttgagaa
     3421 cgaatttaaa ccgcttcacc ttgaaaaaac tacatttttt tcatcgtgaa atggacagag
     3481 gataaattgt tcaataacgg attatccccg ctgacgaaag tgccattttc gaccatcgtc
     3541 acgccttttg tctgcagaaa attaaggcag taccttcatt taagcgtcag gtggatgttt
     3601 ttgctcttat ttcgatcaat gaataaacgg gaaaacaggg gtgttttata tcagcaggat
     3661 ctatgtgaac gctcttcagg acggatcacg cgtaaaaaaa agccagcctg tttccagact
     3721 ggcttttgtg cttttcaagc cggtgttaca tcgctttttt ggtcaactcg ataacgcgca
     3781 gctgcgcgat cgctttggcc agttccgcag acgcctgagc gtaatctacg tcgccgtgag
     3841 agctgctaat gtgctcttca gccttacgtt tcgcttccat ggctcgcgct tcgtcgagat
     3901 cctggccgcg aattgcggtg tcggccagaa cggtcacgtt gccaggctgc acttcaagaa
     3961 tgccgccaga cagatagata aactcttcgt gaccgtgctg tttcacgatg cgaatcatac
     4021 caggcttaat ggcggtgagc agcggtgcgt ggccagggta gatccccagt tcaccttcgc
     4081 tacccgttac ctggattttc tcgaccagac cagagaacat ttgttgctct gcgctgacga
     4141 cgtccaggtg gtaagtcatt gccatatcac cctccgatta aggcgttaaa gttttttggc
     4201 tttttccaca gcttcttcga tggaaccgac catgtagaac gcctgctccg gcaggtgatc
     4261 gtattcgcct tccatgatgc ctttaaagcc acggatggtg tctttcaggg agacgtattt
     4321 acccggagaa ccggtgaata cttctgccac gaagaacggc tgggacagga agcgctggat
     4381 cttacgagca cgcgctacca ccagtttgtc ttcttcagac agttcatcca tacccaggat
     4441 ggcgatgatg tctttcagtt cctgataacg ttgcaggatg gactgaacgc cacgcgcggt
     4501 gtcgtagtgt tcctgaccaa ccaccagcgg gtccagctga cggctggtgg agtccagcgg
     4561 gtcaacggcc gggtagatac ccagagacgc gatctgacgg ctcagtacca cggttgcgtc
     4621 aaggtgcgca aaggtggttg ccggagacgg gtcagtcaag tcatccgcag gtacgtatac
     4681 cgcctgtacg gaggtgatag aaccagtttt ggtggaggtg atacgttcct gcagaacgcc
     4741 catctcttcc gccagggtcg gctgataacc taccgctgaa ggcatacggc ccagcagtgc
     4801 ggatacttcc gtaccggcca gggtgtaacg atagatgttg tcaacgaaca gcagaacgtc
     4861 acgaccttcg tcacggaatt tctcagccat ggtcagaccg gtcagagcaa cgcgcagacg
     4921 gtttcccggc ggctcgttca tctggccata caccagggat actttgtcga taacgttgga
     4981 gtcggtcatt tcgtggtaga agtcgttacc ctcacgagta cgttcaccta cgcccgcaaa
     5041 cacagagtaa ccggagtgct cgatcgcgat gttacgaatg agctccatca tgtttacggt
     5101 tttacctaca cccgcaccac cgaacagacc aactttaccg cccttagcga acggacacat
     5161 caggtcgata actttgatac cggtttccag cagttcctga gagtttgaca gctcttcgta
     5221 ggaaggtgct gcgcggtgaa tcgcccaacg ctcttcttca ccgatctcgc ctttcatgtc
     5281 gaccggttca cccagtacgt tcatgatacg gcccagagtc gctttaccta ccgggacttc
     5341 gatcgggtgt tcgaggtctt ttacatccag accgcgacgc agaccgtcgg aggaacccat
     5401 tgcgatggta cgcacgatac cgccgccgag ctgctgctga acttccagca ccagacgctc
     5461 attaccattt tgcacctcaa gagcatcgta cacgcgcggt acggcatcct gagggaattc
     5521 gacgtcaact acggcgccga ttacctggac aatctttcca gtagccatct taaatcctct
     5581 acgaaataac ctgtttaaac cgcggcggcc cccgagacga tctcggtgag ttcctgagta
     5641 atgctggcct gacgagcttt gttgtatacc aactgcagct ctttaatcag gctgccgcca
     5701 ttgtcggtcg cggctttcat cgccaccata cgggcggcct gctcgctggc caggttttca
     5761 accacgccct gataaacctg agattcgaca taacgacgca gcagggtatc cagcaacgcc
     5821 ttcggatcgg gttcgtacag gtaatcccag gatttatgtt tcagatcatc atcatctgat
     5881 gccggtaacg gcagcagctg gctgatggtc ggaacctgag acatggtgtt aataaatttg
     5941 ttgctgacaa tgtaaagctt gtccagacgg ccttcgtcgt aggcctgcaa catcactttt
     6001 accggaccga tcagttcgga cagggaaggg ttatccccca tgccggtgac ctgggcaaca
     6061 acattgccgc ccacggagtt gaagaacgac acgcctttcg agccgatcat tgcgaggtcg
     6121 cattgaacgc ctttgtcggt ccaggtcttc atttccgcca gcagtttttt gaacaggtta
     6181 atgttcaaac caccgcacaa accacggtcg gtcgacacca ccaggtagcc cacgcgttta
     6241 acgtcgcggt cttccaggta agggtgctta tattccagat taccgtgtgc aaggtgacca
     6301 atcactttgc gcatggtttc tgcataagga cggctggccg ccatgcgatc ctgcgattta
     6361 cgcattttgg aagcggcgac catctccatc gctttagtga tcttttgcgt gttctggacg
     6421 cttgcgatct tactacgtat ctcttttgcg ccggccatga gcttctcctc aatgccttgc
     6481 ggcctgccct aaggcaagcc gccagacgtt accaggattg ggttgctttg aaggaatcga
     6541 ggatgccttt cagcttgcct tcgatttcgt cgttgtagcc accggtctgg ttgatctctt
     6601 gcatcaacgg agcgtgatca cggtcgacgt aagccagcag agcggcttcg aagctgccaa
     6661 ttttcgacag ttcaacatcc gccaggtaac cacgttctgc tgcgaacaga accagagact
     6721 gctgcgcaac ggacatcggc gcatactgtt tctgtttcag cagttcggtc actttctgac
     6781 cgtggtcaag ctgcttacgt gttgcatcgt caaggtcgga tgcaaactga gagaacgctg
     6841 ccagttcacg atactgtgcc agagcggtac ggataccacc ggacagtttt ttcatgatct
     6901 tggtctgtgc tgcaccacca acacgggata cggaaatacc cgggttaacc gcaggacgaa
     6961 taccggcgtt gaacaggttg gtttccagga agatctgacc atcggtaatg gagattacgt
     7021 tggtcggaac gaacgcagaa acgtcacccg cctgagtttc gataatcggc agtgcggtca
     7081 gagaaccggt tttccctttc acttcacctt tggtgaaggc ttcaacgtat tcggcgttaa
     7141 cacgcgcagc acgctccagc agacgagagt ggaggtagaa tacgtcgccc gggaatgctt
     7201 cacgtcctgg cggacgacgg agcagcaggg agatctgacg gtaagcaaca gcctgtttag
     7261 acaggtcatc gtaaatgatc agcgcatctt caccgcggtc acggaagtat tcgcccattg
     7321 cgcaaccggc atacggagcc agatattgca gtgcagcgga ttcagacgcg gttgctacca
     7381 caacgatggt gttagccagt gcgccgtgct cttccagttt acgtaccacg ttagaaatgg
     7441 tggacgcttt ctggccgata gcgacataga tacatttgat accggaatcg cgctggttga
     7501 tgatggcatc gatagccagt gcggttttac cggtctgacg gtcaccgatg atcaattcac
     7561 gctgaccacg accaattggg atcatggagt caacggcttt ataaccggtc tgtaccggct
     7621 gatctacgga ctgacgttcg ataacgcccg gagcgattgc ttctacagca gagaagccgt
     7681 cgtgatccag cggaccttta ccgtcgattg gtgcacccag agtgttaacc acacggccca
     7741 gcaggccacg gccaaccgga acttccagga tacggccagt acacttaact ttcatgcctt
     7801 cggcaaggtc agcgtacgga cccataacaa ccgcacctac agagtcgcgc tcgaggttca
     7861 gtgcgatagc gtaacggtta cccggcaggg agatcatttc accctgcata caatcggcca
     7921 ggccgtgaat gcggataaca ccgtcactta cagaaacaat agtaccttcg ttgtgagctt
     7981 cactcacaac attgaactga gcaatgcgct gcttgatcag ttcgctgatt tcggtggaat
     8041 tcagttgcat gctccagtcc ccttaagact gcaagacgtc tgcaaggcgc tcaagacgac
     8101 cgcgtacgct gccatcaatg accatatcac ccgctcggat gataacgcct gccattacag
     8161 acttatcgat tttgcaattc agcttaactt tgcgtgacag acgtttttcc atcgcagcag
     8221 aaattttcgc gagctgttgt tcactcagtg cggcagcgga aatgacgtct acctcagcgg
     8281 tagcctcact cacggcacga aggtgaataa actgctccag aacatccggg agcgcgttaa
     8341 gacgaccatt ttcagccatt acccgaatca ggttctgacc gttttcgtcc agttgctcac
     8401 cacaaactgc gataaacgac tcggcgagcg tttctggcgc aagcgcgcca gagagaagct
     8461 ctgccatttg ttcgtttttg gttacctcgg cggcaaacgc cagcatgtcc tgccagcgtt
     8521 ctacactttg gtgttcgacg gcaaagtcaa aagctgcttt ggcgtagggg cgagctaccg
     8581 taataaattc agacatcagc ccctccctcc ttacagttca gcgacaagtt tatccacgat
     8641 gtcgctgtta gcagcttcat ccacggaacg ttcgatgatc ttctcggcgc cagcaacagc
     8701 caggatagca acttgcttac gcagctcttc acgggcacgt ttacgctcgg cttcaatttc
     8761 tgcctgcgcc tgggccacga ttttagtacg ttcctgttct gcctcagctt tcgcttcgtc
     8821 cagaatctgc gagcggcgtt tgttcgcctg ctcgatgatt acctgggctt ccgctttcgc
     8881 ttttttcagc tggtcggtcg cgctggcctt tgcaaggtca aggtccttat gtgctcgttc
     8941 tgcggaagca aggccgtcag caatttcttt ttgacgtttt tcgatggctg ccattaatgg
     9001 cggccatacg tacttcatgc agaacagaac gaacaggaca aacgcgatgg cctggccgag
     9061 gattgttgcg ttaagattca cagcacaatg cctctattta gttaacgttc tgatattgct
     9121 ctttaaataa aagcaacgct tactacgcga cagcgaacat cacgtacaga cccagaccta
     9181 cagcgatcat cgggatagca tccaccagac ccataacgat aaagaactga gtacgcagca
     9241 gaggaatcag atcaggttga cgcgctgcgc cttccaggaa tttacccccg aggatgccga
     9301 taccgatcgc agcaccgatt gccgccagac ccatcatcac agcggcagcc atgtacagca
     9361 gatccatatt caggttttcc atgacagtct ccagtttgtt tcagttaaaa cgtagtagtg
     9421 ttggtaaatt aatgttcttc agacgccatc gacagataga cgatcgtcag aaccatgaag
     9481 atgaaggctt gcagcgtaat gatcaggatg tggaaaatgg cccacggcac attcaggatc
     9541 cactgtgacc accacggcaa cagaccagca atcagaatga aaatcagctc accggcatac
     9601 atgttaccga acagtcgcaa accaagtgaa actggtttgg acagcaggct taccccttca
     9661 aggattaagt tgacaggaat gaacgcccag tgattgaacg gctgcagcgt caactctttc
     9721 gtgaagccgc cgatgccttt cattttgatg ctgtagaaca gaatcaggat aaatacgccc
     9781 aatgccatag acagcgttac gttcacgtcc gcagacggaa ccacacgcag tgcaggcaga
     9841 cccagtacat gttcagcaat gtacggcagc aggtcgatag gcagtaaatc catcaggttc
     9901 atcaggaata cccagacgaa gatcgtcagg gccagcggag caatcagctt gcttttgcca
     9961 tggtacatgt ctttcacgct accattaaca aagccgatca ccagctcaat cgcggtctga
    10021 aacttacctg gcacaccgct ggtcgccttt ttggctacgc tacggaataa aaccaggaac
    10081 aacagaccca gcaccaccga gaagaacatg gagtcaatat tgattgtcca gaaggtggct
    10141 ggggggtttt gtggatccac cagcgagaat gtacgcaggt ccagctgaag gttattcagg
    10201 tggtgtccta tgtaatcctg cggcgtcata ttttctgaag ccatgatgcc ttttaccctt
    10261 tgttgttaat tacagccggt gccagtatct gaaccaccag caccaaaacc cacgtaacga
    10321 tcagcggcaa gaataccgcc tttaaaaccg ccaacgccac caccagtaac accaacatcg
    10381 ccagaacttt gaaagcttcg ccaaatgcga atgtccaggc cacccggcct ttcgctggtg
    10441 tatgcgcctg gtgacgccag gcaaatatca taaacaaaac gttaggcaga aagactgcca
    10501 ggcccccgct tattgcagag acgccccaga aggggtcttt gaggctgaac agcaatccac
    10561 ttgctatcac caccagtaac tgaacgagca gaagcttccg agcaacgttt cgactcacga
    10621 gcgacacaga catcacgttt ttcactcctg ctcccttcga ggtatgccgc gtgtcgtata
    10681 aaactttctt taaggcttag agtcaagcat caaaaagcgg tcaaattata cggtgcgccc
    10741 ccgtgatttc aaacaataag tagccaaaag gtgaataaat gtttaaatat ttttccagtg
    10801 catacaattg cgacttttct gctaaccctg ttcgatcatg aaaaactgta aataacgcgt
    10861 aaacactggt gataaagcgt gcttcagatc acatattgcg catgttcgcg cacagcatat
    10921 ttatttactt ggcaaatgat gcctttgcaa gtttatgata tttcagtcta aaaacagata
    10981 ctgttttaat aaatgacatt tacacaacaa aaaccaccca ttgacatttt taataatgtt
    11041 ttaacagcca atgatggttc ttagcgccga tttttagcag actgatattt tcactaatga
    11101 cttattttct gcttaccaaa aaaagccacg ttatcttgtt gatgcaaaag agtgaacgtg
    11161 gcgttaaatg taaccagtta tatcagtaga aaacctggtt gttgttaaca gtctaaccgg
    11221 tcaatttttt atgatttttt tgataaaaat taaattttat ttgctttaat caccaccaga
    11281 tgacgttcgc catccagggc tggaacctga agtttaacca ctgattcgac ctgatattct
    11341 tcgggcaaca aagcgatttc atcttccggc atttgccctt tcagcgcgta gaaacggcct
    11401 tgctcaccag gaagatggtg gcaccagctc accatatcgt tcagagaggc aaaagcgcgg
    11461 ctaattacgc catcaaatgg cggctctgaa ggaaactctt ctaccctgct ctgtactggt
    11521 tcaatattct ccagtttaag ctcatgttgc acctgacgaa ggaaacgcac gcgtttacca
    11581 aggctatcca acagagtgaa atgggcttca ggacgcacga tagagagtgg aatgcctggc
    11641 agtcctggtc cggtgccgac atcgataaac cgttcacctt gcagatacgg tgccaccaca
    11701 atgctatcga gaatatggcg taccagcatc tcattaggat cgcggaccga agtcaggttg
    11761 tacgctttgt tccatttatg cagcatattc acgtaggcaa taagctggtt tttctggtga
    11821 tcggtaagcg aaatacctgc gtctttcagc agtaaggaga gtttgttgag cacggtgatt
    11881 acctgttctt gatgcgttgc ctggtaagcg ggtgcttacc aggcattttt aatgcgttat
    11941 gcgctacgac gcagcatacc ctgttttttc agccacacca gcagaatgga gatggccgca
    12001 ggcgtgacgc cagaaatacg cgaagcctgg ccgatagagg ctggtttgtg atcgttaagt
    12061 ttggcgatca cttcgttaga aagaccggat acctggcggt aatccagtgt cgcgggtagc
    12121 agggtgttct cgttacgcag ctgcttttcg atctcatctt gctggcgcgc gatataacct
    12181 tcgtatttaa cctgaatctc aacctgttcc gccgcctgtt cgtctgtcaa cgcaggggca
    12241 aacggcgtca gcgtggttaa tttttcataa gtcatttccg gacgacgcag cagatcttca
    12301 ccactggctt cacgggaaag cggcgcagtc aggtgagcat tcacttcggc tgcagcttcc
    12361 gccgacgggg ttacccaggt cgatttcaga cgctgacgct cacgctcgat attctcaagt
    12421 ttctcgttaa agcgcgccca acgttcgtca tccaccaggc ccagttcacg accgatttca
    12481 gtcaaacgca gatccgcatt atcttcgcgc agcatcagac gatattctgc gcgcgaggta
    12541 aacatacgat acggttcttt ggttcctaaa gtgcacaggt catcaaccag tacgccgaga
    12601 tacgcctgag aacgcgccgg agcccaacct tctttgtcag cagacagacg ggcagcgtta
    12661 agaccggcca gtaaaccttg cgcagcggct tcttcgtaac cggtagtgcc gttaatctga
    12721 ccagcaaaga acagcccctg gataaacttg ctctccagcg tcggtttcag gtcgcgagga
    12781 tcgaagaagt catactcaat ggcataaccc ggacgcacga tcttcgcgtt ttccatcccc
    12841 tgcatagagc ggacgatttg catctgcaca tcgaacggca ggctggtgga gataccgttc
    12901 ggataaattt cgttagaggt cagtccttcc ggttcaagga agatctgatg ctgatttctg
    12961 tcggcgaagc gcatgacttt gtcttcgatc gacgggcagt agcgtgggcc gacaccttcg
    13021 atcacccctg catacattgg gctacgatcg aggttactgc ggatcacatc atgggttttc
    13081 tcgttggtat gagtgatata acacggcacc tgctggggat gctgggacgc attgcccata
    13141 aacgagaata ccggcattgg gttatcgcca tgctgttgcg ccagcacact aaagtcgata
    13201 gttcgagcat caatacgcgg tggtgtcccg gttttcagac gaccaacgcg cagcggcagt
    13261 tcacgcaaac ggcgagaaag cggaatggac ggcggatcac cagcacggcc accgctgtaa
    13321 ttatccagac cgatatgaat tttaccgtcg aggaacgtcc caacggtgag cacgacggct
    13381 ttggcacgga acttcagtcc catttgggta acagcaccga ccacgcgatc gttttcgaca
    13441 ataagatctt caaccgcctg ctggaagatc atcaggttcg gttggttctc cagcgccgta
    13501 cgtaccgcct gacggtagag cacacgatcc gcctgagctc gggtagcgcg aaccgccggt
    13561 cctttgcttg cgtttagtat cctaaactgg atacccgctt gatcgatcgc tttcgccatc
    13621 agaccgccga gtgcatccac ttcttttacc agatgtccct tcccaatacc gccgatcgcc
    13681 gggttgcagc tcatctgccc cagagtgtcg atattgtgtg tcaaaagcag agtctgttga
    13741 cccatacgcg ccgcggccat cgcggcctcg gtgcctgcat gacccccgcc aatgatgatg
    13801 acgtcaaaag gatccggata aaacatggtg attgcctcgc ataacgcggt atgaaaatgg
    13861 attgaagccc gggccgtgga ttctactcaa ctttgtcggc ttgagaaaga cctgggatct
    13921 tgggtattaa aaagaagatc tatttattta gagatctgtt ctattgtgat ctcttattag
    13981 gatcgcactg ccctgtggat aacaaggatc cggcttttaa gatcaacaac ctggaaagga
    14041 tcattaactg tgaatgatcg gtgatcctgg accgtataag ctgggatcag aatgaggggt
    14101 tatacacaac tcaaaaactg aacaaccgtt gttccttgga taactaccgg ttgatccaag
    14161 cttcctgaca gagttatcca cagtagatcg cacgatctgt atacttattt gagtaaatta
    14221 atccacgatc ccagccattc ttctgccgga tcttccggaa tgtcgtgatc aagaatgttg
    14281 atcttcagtg tttcgcctgt ctgttttgca ccggaatctt tgagttctgc ctcgagttta
    14341 tcgatagccc cacaaaaggt gtcatattca cgactaccaa taccgattgc gccaaagcgg
    14401 accgcagaaa gatcgggctt ctgttcctgc aatgcttcat agaaaggaga aaggttgtcc
    14461 ggaatatctc cggcaccgtg ggtggagctg ataatcagcc agatccccga ggcagataaa
    14521 tcttccaaca gcggaccgtg cagcgtttcg gtggtaaaac ccgcctcttc cagcttttca
    14581 gccaggtgtt ctgctacata ttcggcaccg ccgagggtgc tgccgctgat aagagtgata
    14641 tctgccataa accgccacct ttattaagag tggcgtattg tacgctgtga acgcgttggg
    14701 atctacctgt ggaaaagtat gggattaaaa aagccgatca gggcttgata gtacgcatga
    14761 tcgggttttg caggacgatc aatgtctcgg tggactgaat ttcatcaatt gtttggatct
    14821 tgttgataag tacatgctgg agagcgtcga tcgaacggca cattactttt ataaagatgc
    14881 tgtagtggcc tgttgtgtaa taggcttcag tgacttcatc aaggctttcc agctttgcca
    14941 gcgcggaagg gtagtctttg gcgctcttta atataatgcc gataaagcag cctacgtcat
    15001 aaccgagctg cttcgggctg acatcaatac gcgccccggt aatgatcccc gcctgcttca
    15061 ttttctctac ccgaacgtga atcgtccccg gactgacgcc aaattgtttc gccagttcgg
    15121 cgtaagcggt gcgcgcattg cccattaatg cttccaggat gccacggtcc agattgtcga
    15181 tcagataatt ttccatagga ttttcttatg cagattgatg attcattgta ttttagcctt
    15241 cttttttaat gaatcaaaag tgtgttaggc tttttattga atgattattg catgtgtgtc
    15301 ggtttttgtt gcttaatcat aagcaacagg acgcaggagt ataaaaaatg aaaaccgctt
    15361 acattgccaa acaacgccaa attagcttcg tgaaatctca cttttctcgc caactggaag
    15421 aacgtctggg gctgatcgaa gtccaggcgc cgattcttag ccgtgtgggg gatggcacgc
    15481 aggataactt gtcgggctgt gaaaaagcgg tgcaggtaaa agtgaaagtt ctgcctgatg
    15541 cccagttcga agtggttcat tcactggcga agtggaaacg tcagacctta gggcaacacg
    15601 acttcagcgc gggcgaaggg ctgtacacgc acatgaaagc ccttcgcccc gatgaagacc
    15661 gtctttctcc gttgcactcg gtctatgttg accagtggga ctgggaacgc gtaatgggcg
    15721 acggtgagcg tcaattctcg actctgaaaa gcacggtaga ggcgatctgg gcgggaatta
    15781 aagcaaccga agctgcggtt agcgaagagt ttggcctggc accgttcctg ccggatcaga
    15841 tccacttcgt acacagccag gagttactgt ctcgttatcc ggatcttgat gccaaagggc
    15901 gtgagcgggc gatagcgaaa gatcttggcg cggtattcct tgtcgggatt ggcggcaagc
    15961 tgagcgatgg tcatcgccac gacgtgcgcg caccggatta tgatgactgg agcaccccgt
    16021 cagagctggg tcatgcgggt ctgaacggcg atattctggt gtggaaccct gtactggaag
    16081 atgcgtttga gctttcttcc atggggatcc gcgtggatgc cgacacgttg aagcatcagt
    16141 tggcgctgac cggtgatgaa gatcgtctgc agctggaatg gcatcaggcg ctgctgcgcg
    16201 gtgaaatgcc gcagaccatc ggcggcggta tcggccagtc tcgtttgact atgctgctgc
    16261 tgcaactgcc gcatatcggc caggttcagt gtggagtatg gccagctgct gttcgcgaga
    16321 gcgtcccttc tctgctgtaa taatttatcg ccgccagcgt ctgagcaggc ggcttcgcat
    16381 cccggtatca aagcgccaga tatgatcgaa aatgcgcatg atgccgggtt tgccgtgtgc
    16441 cgacatcgcc acggcatgaa agcgatgctg atgtacccgc tgcagctctt tcactttact
    16501 cgtcacgtcg tcaggcaacc gctgagcgat aaaatcagaa atcaccaccg catcggcatc
    16561 aaaccattcc ctgctttgca agcgttccat aatggcgcga aaacaactgg caagatcggt
    16621 gccgccacga aactgctggc ttaaaaaacg gattgcttgt tcgatgcctt gtgggcctga
    16681 aagctcataa cggacgatct cggtggaaaa tagcataata tagcagcgcc ggttttctgc
    16741 gagagcaatg cgcatcaagg ccaggcagaa cgctttcgca cactgttcat taaagccgcc
    16801 cattgagccg gaagtatcca cacagacaat aaacggcccg cgcggctgtt catcgtaatc
    16861 tttatgtacc accggacgtt cgatcacttt ttcacgccac gactcaccgt gcaggcgata
    16921 ggtgagcaac tgtttttcca ccagccgacg gtaaaactca tactccagtt ccgttatccc
    16981 tagtgtcgcc agttctggcg gcaggagacg taaaatatca tcgctttgtt gcagaccatc
    17041 aacctgctca ggaaccgtcg ccggttcgcg caccatggtg cggaaggttt ccatctgcgc
    17101 atcgttgcgc ggtattgatt tggcttcccg agaacgcccc agctgctctg ccaggcgttt
    17161 cagttccggc tgttcgttaa gaaattcacc gtatttcaca atcaactgat agtcgccacg
    17221 tttaagctga ccggcgctca tatcccacag acgaccagct gcagtattgt tatctgcgag
    17281 aatcggttca agttgtccgc tcagcgtcat gcgttcctga acttcactca acagttgttc
    17341 gcgttcttct tctaatagct gttgatttaa cgtcgttgct tgcacgatca gacttaaacg
    17401 ccaacgctgg agaaaaagcg tgtgtaacgc tgaagtgatc gtgctgttag catcaaccaa
    17461 ctgtcgggct tgttctgccc atggagaatt cagacgatgc agtaagtcca ggatctgtgg
    17521 tagctgcaca ataaactgtg gcgtggagag gagctggctt tgctgatagc acatcacctc
    17581 ttcggtgagt tccggcggga ctcgggcatc tttcagccga ctgcgcagcg cttcacgcca
    17641 gcggggaaca tcatcagtga ttgccgcctt caggcgtggg aatttttcaa agaagaccgc
    17701 cagctgcggt gaggccagca gtgcgatgat catctcttcg atcaatccct cttcgctgac
    17761 ggccagcatc acattaagcg tatccagcgt tagcattgtt gtgcctggcg aatctgtgca
    17821 ccgacatcct gtaggctggc ttcaatgcga cctaaccagt cgccagggat aaacagacat
    17881 ttttgctgtt cgctgaataa agcgtgttgc ttgcgccagt cgctttcaag ctcttccagt
    17941 tgttgtttta tttcacctgg cagaccttca gccgatgaac cgggaagtgc cagcgtactg
    18001 ccttgtaaac ttacatcgcg aacgacaaga tgttgggcgc tatcaacttc cagattcagt
    18061 ttctgggcaa agccgatacc gttcagttta ccgcgaattt caccaccttt gctcagccac
    18121 tgttccagcg cgctacgctc aaaggagata tgaaccactt ccatatcatg cagttttaac
    18181 ggtttttgca gcagtagagt cagagtggaa gcagtaacgt taacaggaag ttgatactgc
    18241 tgacgacggc tgaaaatgcc gcccagacga attaccgtta aggctgtttt atcgctttgt
    18301 tgctgctgga gttgcaggtg acgttgcaca atcgcgccaa ggcgagtcaa catcccttgc
    18361 tgttgccagg cgtgaccggt catcaataca tcaatttgtt gttgtatcaa attcaggctt
    18421 tgcgcgtcat accacaggca atctttcagc aaaatgagat ctaccggggc aacagcactg
    18481 cgaccgctaa aaaaggcgct ggcctgcaat aatcggatcg cttttttcca gcgacgatcc
    18541 gagacataag gcgcatccgg taatttatcc agttgctggc gcagcataaa aatcagctca
    18601 aatacatgat cgggcagcgt aatttcacca atctctttct gccagcgttc atattcttca
    18661 tctgtgacct gcaaggcatc aggaaccgga ttgtcgtttt catcctgttg actggtcagc
    18721 atggagcgga aattcgcttt atcctgcact ttatctaacc acagacgaat cagcatgcgg
    18781 tcatataacg cttccagact gctgtctgct tccggcagct cgttggaggc cgccaccagc
    18841 agacgcatcg ggattttttc tacgtgtgca ccgttgcgga actggcgctc gttaatggcg
    18901 gtgagcaagg tattaagaat tgccgggccc gctttccaga tctcatccag aaagacaatt
    18961 tctgcttccg gcaggtaacc gctggttaaa cgttcatagc gcccttcatc ttttagcgcc
    19021 tgaatagaaa ggggaccaaa aacttcttcc ggcgtggaga agcgggtcat cagatattca
    19081 aacgcgcggg cattctgaaa ggcgaatttt aagcgccggg cgatcaaact tttggcaata
    19141 cctggcgggc caaggaggaa cacactttca ccacttaatg ccgctaataa acacaagcgg
    19201 atggcgtggc tacgttcata aagccccttt tccaacgaac tgctcaggcg ggaaattctt
    19261 tccgctaata aatgagggtg agccataatg aagtggcgtc ctttcgtcaa aagttctgcg
    19321 taaattgcga gtatagacgt ttcttgctgg tggctaaaat agtctcaaag ggggggtatt
    19381 tttctttgag ccaggttaat gtggccgcat ttaggagtac gattttgccg ttaatcgtgc
    19441 atactgtgcg cttttttgtg ggccaaggga ctaagcacac atttcatatt tcaacgaaag
    19501 actagtctat gagcactgat aataagcaat cattgcccgc gattaccctc gcggcgattg
    19561 gagttgtcta cggcgatatt ggtaccagcc cgttatatac acttcgtgaa tgtttgtccg
    19621 gccagtttgg ttttggcgtt gaacgcgatg ccgtgtttgg ctttttatcg ctgatcttct
    19681 ggctgctaat ctttgtggtt tccattaaat atctcacctt cgtgatgcgg gcagataacg
    19741 ccggtgaggg ggggatcctg acgttgatgt cgcttgccgg gcgtaatacg tcggcgcgaa
    19801 ccacatcaat gctggtgatt atggggctaa tcggcggcag ctttttctat ggtgaagtcg
    19861 tcataacacc cgctatttcg gtgatgtcag ccattgaagg tctggaaatc gtcgccccgc
    19921 agctggatac ctggatagtt cccctctcaa ttatcgttct cactttatta tttatgattc
    19981 aaaaacatgg cacggctatg gtcggtaagc tgtttgcgcc gatcatgctg acctggtttt
    20041 tgattctggc aggactgggg ttacgtagca ttattgctaa cccggaagtg ctgcatgcgc
    20101 tgaatccaat gtgggcggtg catttcttcc ttgaatacaa aacggtttct tttattgcat
    20161 taggggcagt ggtgttgtcg attacggggg tcgaggcgct gtatgctgat atggggcact
    20221 ttggtaagtt ccctattcgc ctggcgtggt tcaccgtcgt attaccttcc ttgaccctta
    20281 attacttcgg tcagggagcg ctgttgttaa agaacccgga agcgattaag aacccgttct
    20341 tcctgttggc accggactgg gcgctgatcc cgctgctgat catcgccgca ctggcgacgg
    20401 taattgcctc gcaggcggtt atctctggcg tcttctcatt gacgcgtcag gcggtacgtc
    20461 tgggatattt atcgccgatg cgcattattc acacctccga aatggagtca gggcaaatct
    20521 atattccgtt tgtgaactgg atgctctatg tcgcggtcgt gattgtgatt gtcagctttg
    20581 agcactccag caacctggcg gcggcgtacg ggattgcggt gaccggaacc atggtgctga
    20641 cgtctattct ctcgactacc gtggcacgtc agaactggca ctggaataag tattttgttg
    20701 cgctgatcct gattgctttc ctttgtgtcg atattccatt gttcaccgct aacctcgata
    20761 aactgctctc cggcggctgg ttgccattga gcctcggtac ggtgatgttt atcgtgatga
    20821 ccacctggaa gagcgagcgt ttccgcttgc tgcggcggat gcatgaacat ggtaactctc
    20881 tggaagcgat gattgcttcg ctggagaaat caccgcctgt tcgcgtgccc gggaccgcgg
    20941 tgtatatgtc gcgtgcaatc aacgtcattc cctttgcgct gatgcataac cttaaacata
    21001 acaaggtatt gcatgagcgg gtgattctgt taactctgcg taccgaagac gcgccatatg
    21061 tccataatgt ccgtcgggta cagattgaac aactgtcgcc cactttctgg cgcgtggtgg
    21121 caagttatgg ttggcgagaa acgccaaacg tggaagaagt tttccaccgc tgcggtctgg
    21181 aaggattaag ttgccggatg atggaaacct ccttctttat gtcgcatgag tcgttgatcc
    21241 tcggcaaacg cccgtggtat ttgcgtctgc gcggcaagct gtacttgctg ctgcaacgta
    21301 atgcgctgcg tgcaccagat caatttgaaa tcccgccaaa cagggttatc gaactgggta
    21361 ctcaggtcga aatctaacgc cagacgcctc ctttctccat aagggggcgt ttttgttttc
    21421 atggttaatc accatgtaaa acgtttcgag gttgatcaca tttccgtaac gtcacgatgg
    21481 ttttcccaac tcagtcagga ttaaactgtg ggtcagcgaa acgtttcgct gatggagaaa
    21541 aaaatgaaaa aaggcaccgt tcttaattct gatatttcat cggtgatctc ccgtctggga
    21601 cataccgata cgctggtggt gtgtgatgct ggtttaccca tctccaaaag tacaacgcgt
    21661 atcgatatgg cattaaccca gggtgtacct tcttttatgc aggtgctggg cgtcgttaca
    21721 aatgaaatgc aggtcgaggc tgccattatc gcggaagaga tcaaacaaca taatccgcaa
    21781 ctccacgaaa cgttgctcac tcaccttgag cagctgcaaa aacaccaggg aaataccatt
    21841 gaaattcgtt acaccacgca tgaacaattc aaacaacaaa ccgcagaaag tcaggcggta
    21901 attcgcagcg gagaatgttc tccgtatgcg aatatcattc tctgtgctgg cgtgacgttc
    21961 tgaggccgtc atggaagcat tacttcagct taaaggcatc gataaagcct tcccgggcgt
    22021 aaaagccctc tcgggcgcag cgttaaatgt ctatccgggc cgcgtgatgg cgctggtggg
    22081 cgaaaacggc gcgggtaaat ccaccatgat gaaagtgctt actggtatct atgctcgcga
    22141 tgccggcacg cttttatggc tggggaaaga aacgacattt accgggccga aatcttccca
    22201 ggaagccggg attgggatta tccatcagga actgaacctg atcccgcagt tgaccattgc
    22261 cgaaaacatt ttcctcggtc gtgagtttgt taatcgcttt ggcaaaattg actggaaaac
    22321 catgtatgcc gaagcggata aattgctggc taaacttaac ctgcgcttta aaagcgacaa
    22381 gctggtgggc gatctttcca tcggtgacca gcaaatggtt gaaatcgcca aagtgctgag
    22441 ctttgagtcg aaagtcatca ttatggatga accgaccgat gcgctgaccg ataccgaaac
    22501 cgaatccctg ttccgcgtca tccgcgaact aaaatcgcaa ggccgcggta ttgtctatat
    22561 ctcccaccgc atgaaagaaa tcttcgagat ttgcgatgac gttaccgttt ttcgtgacgg
    22621 gcaatttatt gctgagcgcg aagtggcatc actgaccgaa gattcgctga ttgagatgat
    22681 ggtgggtcgc aagctggaag atcaatatcc gcacctgggc aaagcgccgg gagatatccg
    22741 cctgaaagtc gataatctct gcggacctgg cgttaacgat gtctctttta ctttacgcaa
    22801 aggcgaaatt cttggcgtct ctggtttgat gggcgcaggt cgtaccgaac tgatgaaagt
    22861 gctctacggc gcactaccgc gcaccagcgg ttacgtcacc ctggatgggc atgaagtcgt
    22921 tacccgttca ccgcaggatg gcctggcaaa cggcattgtg tatatctccg aagaccgtaa
    22981 acgtgacggt ttagtgttgg gcatgtcagt aaaagagaac atgtcgctga cagcgctgcg
    23041 ctacttcagc cgcgctggcg gcagtttgaa gcatgccgat gaacagcagg ctgtgagtga
    23101 tttcattcgt ctgtttaatg tgaaaacacc gtcgatggaa caggcaattg gtctgctttc
    23161 cggtggcaat cagcaaaaag tggcgattgc ccgcggtctg atgacacgcc ccaaagtgtt
    23221 gatccttgat gaacctaccc gtggcgtaga tgtcggcgcg aaaaaagaga tctatcaact
    23281 gattaaccag ttcaaagccg atggcttgag catcattctg gtgtcatcgg agatgccaga
    23341 agtattaggc atgagcgatc gcatcatcgt catgcatgaa gggcatctca gcggggaatt
    23401 tactcgtgag caggccaccc aggaagtgtt aatggctgcc gctgtgggca agcttaatcg
    23461 cgtgaatcag gagtaaaaaa atgacaaccc agactgtctc tggtcgccgt tatttcacga
    23521 aagcgtggct gatggagcag aaatcgctta tcgctctgct ggtgctgatc gcgattgtct
    23581 cgacgttaag cccgaacttt ttcaccatca ataacttatt caatattctt cagcaaacct
    23641 cggtgaacgc cattatggcg gtcgggatga cgctggtgat cctgacgtcg ggtatcgact
    23701 tgtcggtagg ttctctgttg gcgctgaccg gcgcagttgc tgcatctatc gtcggcattg
    23761 aagtcaatgc gctggtggct gtcgctgccg ctctcgcgtt aggtgccgca attggtgcgg
    23821 taaccggggt gattgtagcg aaaggtcgcg ttcaggcgtt tatcgctacg ctggttatga
    23881 tgcttttact gcgcggcgtg accatggttt ataccaacgg tagcccagtg aataccggct
    23941 ttactgagaa cgccgatctg tttggctggt ttggtattgg tcgtccgctg ggcgtaccga
    24001 cgccagtctg gatcatgggg attgtcttcc tcgcggcctg gtacatgctg catcacacgc
    24061 gtctggggcg ttacatctac gcgctgggcg gcaacgaagc agcaacgcgt ctttctggta
    24121 tcaacgtcaa taaaatcaaa atcatcgtct attctctttg tggtctgctg gcatcgctgg
    24181 ccgggatcat tgaagtggcg cgtctctcct ccgcacaacc cacggcgggg actggctatg
    24241 agctggatgc tattgctgcg gtggttctgg gcggtacgag tctggcgggc ggaaaaggtc
    24301 gcattgttgg gacgttgatc ggcgcattaa ttcttggctt ccttaataat ggattgaatt
    24361 tgttaggtgt ttcctcctat taccagatga tcgtcaaagc ggtggtgatt ttgctggcgg
    24421 tgctggtaga caacaaaaag cagtaataac gactacagga catcttgaat atgaacatga
    24481 aaaaactggc taccctggtt tccgctgttg cgctaagcgc caccgtcagt gcgaatgcga
    24541 tggcaaaaga caccatcgca ctggtggtct ccacgcttaa caacccgttc tttgtatcgc
    24601 tgaaagatgg cgcgcagaaa gaggcggata agcttggcta taacctggtg gtgctggact
    24661 cccagaacaa cccggcgaaa gagctggcga acgtgcagga cttaaccgtt cgcggcacca
    24721 aaattctgct gattaacccg accgactccg acgcagtagg taatgctgtg aagatggcta
    24781 accaggcgaa catcccggtt atcactcttg accgccaggc aacgaaaggt gaagtggtga
    24841 gccacattgc ttctgataac gtactgggtg gcaaaatcgc cggtgattac atcgcgaaga
    24901 aagcgggtga aggtgccaaa gttatcgagc tgcaaggcat tgctggtaca tccgcagccc
    24961 gtgaacgtgg cgaaggcttc cagcaggccg ttgctgctca caagtttaat gttcttgcca
    25021 gccagccagc agattttgat cgcactaaag gtttgaacgt aatgcagaac ctgttgaccg
    25081 ctcatccgga tgttcaggct gtattcgcgc agaatgatga aatggcgctg ggcgcgctgc
    25141 gcgcactgca aactgccggt aaatcggatg tgatggtcgt cggatttgac ggtacaccgg
    25201 atggcgaaaa agcggtgaat gatggcaaac tagcagcgac tatcgctcag ctacccgatc
    25261 agattggcgc gaaaggcgtc gaaaccgcag ataaagtgct gaaaggcgag aaagttcagg
    25321 ctaagtatcc ggttgatctg aaactggttg ttaagcagta gttttaatca ggttgtatga
    25381 cctgatggtg acataaatac gtcatcgaca gatgaacgtg taatataaag aaaagcaggg
    25441 cacgcgccac cctaacacgg tggcgcattt tatggacatc ccgaatatgc aaaacgcagg
    25501 cagcctcgtt gttcttggca gcattaatgc tgaccacatt cttaatcttc aatcttttcc
    25561 tactccaggc gaaaccgtaa ccggtaacca ctatcaggtt gcatttggcg gcaaaggcgc
    25621 gaatcaggct gtggctgctg ggcgtagcgg tgcgaatatc gcgtttattg cctgtacggg
    25681 tgatgacagc attggtgaga gcgttcgcca gcagctcgcc actgataaca ttgatattac
    25741 tccggtcagc gtgatcaaag gcgaatcaac aggtgtggcg ctgatttttg ttaatggcga
    25801 aggtgagaat gtcatcggta ttcatgccgg cgctaatgct gccctttccc cggcgctggt
    25861 ggaagcgcaa cgtgagcgta ttgccaacgc gtcagcatta ttaatgcagc tggaatcacc
    25921 actcgaaagt gtgatggcag cggcgaaaat cgcccatcaa aataagacta tcgttgcgct
    25981 taacccggct ccggctcgcg aacttcctga cgaactgttg gcgctggtgg acattattac
    26041 gccaaacgaa acggaagcag aaaagctcac cggtattcgt gttgaaaatg atgaagatgc
    26101 agcgaaggcg gcgcaggtac tgcatgaaaa aggtatccgt actgtactga ttactttagg
    26161 aagtcgtggt gtatgggcta gcgtgaatgg tgaaggtcag cgcgttcctg gattccgggt
    26221 gcaggctgtc gataccattg ctgccggaga tacctttaac ggcgcgttaa tcacggcgtt
    26281 gctggaagaa aaaccattgc cagaggcgat tcggtttgcc catgctgccg ctgcgattgc
    26341 cgtaacacgt aaaggcgcac aaccttccgt accgtggcgt gaagagatcg acgcattttt
    26401 agacaggcag aggtgacgct tggctacaat gaaagatgtt gcccgcctgg cgggcgtttc
    26461 tacctcaaca gtttctcacg ttatcaataa agatcgcttc gtcagtgaag cgattaccgc
    26521 caaagttgaa gcggcgatta aagaactcaa ttacgcgcca tcagccctgg cgcgtagcct
    26581 caaactcaat caaacacata ccattggcat gttgatcact gccagtacca atcctttcta
    26641 ttcagaactg gtgcgtggcg ttgaacgcag ctgcttcgaa cgcggttata gtctcgtcct
    26701 ttgcaatacc gaaggcgatg aacagcggat gaatcgcaat ctggaaacgc tgatgcaaaa
    26761 acgcgttgat ggcttgctgt tactgtgcac cgaaacgcat caaccttcgc gtgaaatcat
    26821 gcaacgttat ccgacagtgc ctactgtgat gatggactgg gctccgttcg atggcgacag
    26881 cgatcttatt caggataact cgttgctggg cggagactta gcaacgcaat atctgatcga
    26941 taaaggtcat acccgtatcg cctgtattac cggtccgctg gataaaactc cggcgcgcct
    27001 gcggttggaa ggttatcggg cggcgatgaa acgtgcgggt ctcagcattc ctgatggcta
    27061 tgaagtcact ggtgattttg aatttaacgg cgggtttgac gccatgcgcc aactgttatc
    27121 acatccgctg cgtcctcagg ccgtctttac cggaaatgac gctatggctg ttggcgttta
    27181 ccaggcgtta tatcaggcag agttacaggt tccgcaggat atcgcggtga ttggctatga
    27241 cgatatcgaa ctggcaagct ttatgacgcc accattaacc actatccacc aaccgaaaga
    27301 tgaactgggg gagctggcga ttgatgtact catccatcgg ataacccagc cgacccttca
    27361 gcaacaacga ttacaactta ctccgattct gatggaacgc ggttcggctt agatttacgc
    27421 tgtcttttga tcaaattatt accatcggtt gttttcagaa gcatgaacat tgctgctgaa
    27481 gcaacagtaa taatgcccat cgtgataaac gtatagtgga attgttcgac agtcgttgtg
    27541 ccttccatcc cttcataaac gcgaaggacg gccgcactta cagcaacgcc taaactaatc
    27601 gacagttgct gcgtgaccgc cagaacactg ttaccgctgc tggcgttgtc atcggtcaga
    27661 tcggcaagtg tgatggtatt catcgcggta aattgcgtcg acatagccat ccctaatata
    27721 aacaacggca agatcagcat ccatatcgcc attgccggtg attgcaaaga gaactgagcg
    27781 atcattagcc caataatcac cgtgatcccc actaacgtat ggcgatagcc cagacgacgt
    27841 aagacttggg taaccatcga ttttgcaata atggaaccta acgctgtcgg tgccatcata
    27901 cagccggcaa taaacgcctg ataaccaaat cctacctgta acatcaatgg cataaggaac
    27961 ggtacacaac cggtccccag acgggttgca atattgccta cgataccgat cgagaaagtg
    28021 cgggttttaa ataaatctaa tgaaattaat gggtttggcg tgtgtcgcgc atggagaata
    28081 tagagaagca gtaacccgat gctggtgaca attaccgtca aggcaatcca gctggcgaca
    28141 atcttttccc cgaatagctc tattccgctt gagaagagaa caaggctgag gccaaacagc
    28201 aaaaagccag tgatatcgaa tctgcgtcgt gcggtggtga aattgggcat atgtttgcgc
    28261 gcgtaaagaa ggcccgcaat acctatgggg atattgatta aaaatatcca gtgccaggtt
    28321 gcccaggtga ccagcacgcc gccaagaacg gggcctaaaa ttggccccac cagacccggc
    28381 atggcgacaa aattcaatac tggaagaagt tcattacgag gataagcgcg cagtaaggcc
    28441 agccgagcaa caggcatcat cattgcgccg cctatcccct gaataacccg gaagacaacc
    28501 agctgtggta gcgaattaga aagtgcgcag gccagagaac ccaatgtgaa cagactcacg
    28561 gcaagggtaa aaatgcgacg cgtaccgaag cgatcggcta gccatccgct taccggaata
    28621 agcatcgcca ccgtcagcgt ataactgatg atggctgatt gcatcgcgag aggagaacga
    28681 ttaaggctat gagcgattgc gggtaaggcg gtattcagaa tagtggcatc aagtgcctgc
    28741 atgaagaagg ccatcgccgc gatccacggc aaacccgcca tactgcgatt ctttttatcg
    28801 ctcattcaat gtcctgttat cgggttgtca cttatcaggt gagcgtagca gcgcctgaca
    28861 agctttaaat gccgcgtcgc catcgctttg gacaatcgca tcgacaatcg cctggtgcag
    28921 atccagcttt atcactgtgt cgctggtaat tgacgtgaag taagtgtgat aaaccgaatg
    28981 gaatagcgag gcgaatgagg tcaaaaacgg attggcgctc atttcataga tatgctcatg
    29041 ccaggccata tcgacttcga tccagcgttc acggcgaaag ttctctttta atgccgccat
    29101 ttcggccatt aacgtattga gatgcgcctt ctgttccgcg gtgccaaccg ttgcagccag
    29161 taggcaagct tgcggctcca gacagatacg cataaccaga aagtgatcga tgacctgatg
    29221 aaagttctct tctgtcatcc accaggtaag caattcctga tcaagaaaat tccagtttga
    29281 ttgtggcatg acccgggtac caattcgcgg tcgcggtaaa accatccctt ttgccgttaa
    29341 cgttttgacc gcttcgcgta ccgctgtacg actcactcca aattgctcgc ccagctcaat
    29401 ctcaccaggc aaaatggtgc cgggttcata ttcacctttt aagatgcgtt gcgccagctt
    29461 ctcagccaga acatacgaaa ggtttttctg tgcagctaac tgttgtgcgc ttaaaggcat
    29521 tactaatctt cctttttctt tttattcctc cttagtatgc caccaggaag tgtgattacg
    29581 gttgcaaaaa cggcaaattg cttgttttat ggcacattaa cggggctttt gctgaaaaa
//
LOCUS       DAJOWB010000049        29573 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJOWB010000049 DAJOWB010000000
VERSION     DAJOWB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.049"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(71..2140)
                     /gene="glyS"
                     /locus_tag="OHO85_002361"
     CDS             complement(71..2140)
                     /gene="glyS"
                     /locus_tag="OHO85_002361"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCQ2671725.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2150..3061)
                     /gene="glyQ"
                     /locus_tag="OHO85_002362"
     CDS             complement(2150..3061)
                     /gene="glyQ"
                     /locus_tag="OHO85_002362"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCQ2671726.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3156..3455)
                     /locus_tag="OHO85_002363"
     CDS             complement(3156..3455)
                     /locus_tag="OHO85_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCQ2671727.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3630..4625
                     /gene="wecH"
                     /locus_tag="OHO85_002364"
     CDS             3630..4625
                     /gene="wecH"
                     /locus_tag="OHO85_002364"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCQ2671728.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4667..5104)
                     /locus_tag="OHO85_002365"
     CDS             complement(4667..5104)
                     /locus_tag="OHO85_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCQ2671729.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5150..5491)
                     /locus_tag="OHO85_002366"
     CDS             complement(5150..5491)
                     /locus_tag="OHO85_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2671730.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5660..7114)
                     /gene="xylB"
                     /locus_tag="OHO85_002367"
     CDS             complement(5660..7114)
                     /gene="xylB"
                     /locus_tag="OHO85_002367"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCQ2671731.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLHLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7186..8508)
                     /gene="xylA"
                     /locus_tag="OHO85_002368"
     CDS             complement(7186..8508)
                     /gene="xylA"
                     /locus_tag="OHO85_002368"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCQ2671732.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGVFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8874..9866
                     /gene="xylF"
                     /locus_tag="OHO85_002369"
     CDS             8874..9866
                     /gene="xylF"
                     /locus_tag="OHO85_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2671733.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9944..11485
                     /gene="xylG"
                     /locus_tag="OHO85_002370"
     CDS             9944..11485
                     /gene="xylG"
                     /locus_tag="OHO85_002370"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCQ2671734.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11463..12644
                     /gene="xylH"
                     /locus_tag="OHO85_002371"
     CDS             11463..12644
                     /gene="xylH"
                     /locus_tag="OHO85_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCQ2671735.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12722..13900
                     /gene="xylR"
                     /locus_tag="OHO85_002372"
     CDS             12722..13900
                     /gene="xylR"
                     /locus_tag="OHO85_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HCQ2671736.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(14008..14832)
                     /locus_tag="OHO85_002373"
     CDS             complement(14008..14832)
                     /locus_tag="OHO85_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCQ2671737.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15152..17182
                     /gene="malS"
                     /locus_tag="OHO85_002374"
     CDS             15152..17182
                     /gene="malS"
                     /locus_tag="OHO85_002374"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCQ2671738.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17360..18613
                     /gene="avtA"
                     /locus_tag="OHO85_002375"
     CDS             17360..18613
                     /gene="avtA"
                     /locus_tag="OHO85_002375"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCQ2671739.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18764..19237)
                     /locus_tag="OHO85_002376"
     CDS             complement(18764..19237)
                     /locus_tag="OHO85_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCQ2671740.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVNAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19339..20187)
                     /gene="yiaJ"
                     /locus_tag="OHO85_002377"
     CDS             complement(19339..20187)
                     /gene="yiaJ"
                     /locus_tag="OHO85_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCQ2671741.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            20388..21386
                     /gene="yiaK"
                     /locus_tag="OHO85_002378"
     CDS             20388..21386
                     /gene="yiaK"
                     /locus_tag="OHO85_002378"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCQ2671742.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21398..21865
                     /locus_tag="OHO85_002379"
     CDS             21398..21865
                     /locus_tag="OHO85_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCQ2671743.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHKSFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            21983..22456
                     /gene="yiaM"
                     /locus_tag="OHO85_002380"
     CDS             21983..22456
                     /gene="yiaM"
                     /locus_tag="OHO85_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HCQ2671744.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            22459..23736
                     /gene="yiaN"
                     /locus_tag="OHO85_002381"
     CDS             22459..23736
                     /gene="yiaN"
                     /locus_tag="OHO85_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HCQ2671745.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            23749..24735
                     /gene="yiaO"
                     /locus_tag="OHO85_002382"
     CDS             23749..24735
                     /gene="yiaO"
                     /locus_tag="OHO85_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HCQ2671746.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            24739..26235
                     /gene="lyxK"
                     /locus_tag="OHO85_002383"
     CDS             24739..26235
                     /gene="lyxK"
                     /locus_tag="OHO85_002383"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HCQ2671747.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTVHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            26232..26894
                     /gene="ulaD"
                     /locus_tag="OHO85_002384"
     CDS             26232..26894
                     /gene="ulaD"
                     /locus_tag="OHO85_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCQ2671748.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGA
                     RA"
     gene            26887..27747
                     /locus_tag="OHO85_002385"
     CDS             26887..27747
                     /locus_tag="OHO85_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCQ2671749.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTG
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            27741..28436
                     /gene="araD"
                     /locus_tag="OHO85_002386"
     CDS             27741..28436
                     /gene="araD"
                     /locus_tag="OHO85_002386"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCQ2671750.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDDTRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(28468..28569)
                     /locus_tag="OHO85_002387"
                     /pseudo
     CDS             complement(28468..28569)
                     /locus_tag="OHO85_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            28573..28761
                     /locus_tag="OHO85_002388"
                     /pseudo
     CDS             28573..28761
                     /locus_tag="OHO85_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28783..29523)
                     /locus_tag="OHO85_002389"
     CDS             complement(28783..29523)
                     /locus_tag="OHO85_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HCQ2671751.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNKNT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
BASE COUNT         7281 a         7605 c         7428 g         7259 t
ORIGIN      
        1 aaggttgctg tattatttag cgataataaa aaaagcctgc cagatggcag gctttttaat
       61 aacggcgtta ttactgcaac agcgaaatat ccgcaacgcg caggaacagt tcgcgcagtt
      121 tctccagcat ggtcagacgg ttgagacgca attctttgtc atcaaccatg accatcactt
      181 tatcgaagaa agcatcaacc ggctcacgca gctctgccag ttcgaccagc gcatcctggt
      241 aacgaccttc cgcaaagtac ggctccagct tgtcacgtag caccacaacc tgcatcgcca
      301 gtttaatttc ttccggctct ttcagggtag aggcattcac gcggtcgctc agcacttcgt
      361 cagatttcgc caaaatgttg gagacacgct tgttcgccgc cgccagtgca gcagctgctt
      421 caagggtacg gaagtgcgat accgctttca tgcgggcatc gaaatcagcc ggacgagtcg
      481 gacgacgcgc cagtaccgcc tggatggtgt caacggtgta accttcgtcc tgataccagg
      541 cgcggaagcg accgagcata aagtcgataa catcatcaac cacgttggcg ttagtcagct
      601 tatcgccata cagacgcact gcttcttcgg tcagcgtttg cagatcaagg ttgaggttct
      661 tctcaacgat aattcgcagc acgccaagtg cggcacgacg cagcgcaaac gggtctttgt
      721 cgcctttcgg atgctggccg ataccgaaga tacccgccag agtatccatc ttgtcagcaa
      781 tcgccagcgc acaggctacc gggttagacg gcaggtcatc accggcaaag cgcggctgat
      841 actgctcatt cagcgccacc gcgacatctt ccgcttcgcc atcgtgacgc gcatagtgca
      901 tccccataac gccctgggtg tcggtgaact cgaagaccat gttggtcatc aggtcacact
      961 tagacagcag gcccgcacgg gttgcgtggt taacgtcagc gccaatctgt tcagcaatcc
     1021 agccagccag cgcctggatg cggtcagttt tgtcacgcag cgtacccagc tgttgctgga
     1081 acagcacggt ttgcaggcgc ggcaggttat cttccagacg ttttttacgg tcggtgttga
     1141 agaagaactc ggcatccgcc agacgcggac gaacgacttt ctcgttaccg gagataatct
     1201 gctgcggatc tttcgattcg atgttggcaa cgaagataaa gttcggcagc agtttgccgt
     1261 cgttcgcata caccgggaag tatttctggt cacctttcat ggtgtaaacc agcgcttcag
     1321 ccggcaccgc gaggaatttc tcttcgaatt ttgcggtcag aacgaccggc cactccacca
     1381 gcgaagccac ttcttccagc aggctttcgc ttaagtcagc gttaccgcca atcttacgcg
     1441 ctgcttcttc ggcatcggct ttaatcttcg ctttacgttc ttcgtaatcg gcgatgactt
     1501 tcccgcgctc acgcagaatt tccggatact gatcggcgtt gtcgatggtg aattccggtt
     1561 cgcccataaa gcggtggccg cgaatcacgc gatcggactg aatgcccaga atggttgccg
     1621 gaatgacttt gtcgcccagc agcagggtca cggtgtgcac cggacgcacg aagtgcacgt
     1681 cgcttgcgcc ccaacgcatc agtttcggga tcggcagttt cgccagagaa gtcgcaacca
     1741 tattcggcag cagtgcttcg gtgctttcgc ccttcacatg ggcgcgatac agcagccatt
     1801 cgcctttatc ggtagtcaga cgctcagcct ggtcaacggt aataccgcaa ccacgcgccc
     1861 aaccttctgc cgctttgctc ggtttgcctt cagcgtcgaa cgcctgggca atcgccgggc
     1921 cgcgtttttc gatttcacga tccggttgcg cttccgccag gttagctact ttcagcgcca
     1981 gacgacgcgg agcagcaaac cattgaacgg tgccgtgtgc gaggccagcg ttatccagct
     2041 ccgcagtaaa attcgcagca aaggactcag ccaggctgcg cagtgctttt ggtggcagct
     2101 cttcagtgcc gatttccacc agaaaagttt tctcagacat agccgcctct tacttatctt
     2161 tgttgcacat cgggaagccg agggcttcac gggaagcgta gtatgcttct gccactgctt
     2221 tggtcagggt gcgaatgcgc agaatgtagc gctgacgctc ggtgacggag atggctttac
     2281 gcgcatccag caggttgaag ctgtgggcgg ctttcagaat acgctcgtag gctggcagcg
     2341 gcagcggatt ttccagcgcc agcagctgct gcgcttcttt ctcgtactgc tcaaagcagg
     2401 tgaacaggaa gtccacatcc gcgtattcga agttgtaagt ggactgctcc acttcgttct
     2461 gatggaacac gtcgccgtag gtggttttac ccagcgggcc gtcgctccag accaggtcgt
     2521 aaacgctgtc tacgccctga atgtacatgg ccagacgttc cagaccgtag gtgatctcgc
     2581 cggtaaccgg tttacactcc agaccaccaa cctgctggaa gtaagtgaac tgcgtcactt
     2641 ccatgccgtt tagccacact tcccagccca gtccccaggc acccagagtc gggttttccc
     2701 agttatcttc cacgaaacgg atgtcgtgaa tagtcgggtc catgcccagc tctttcagag
     2761 aaccgaggta cagctcctga atattgtccg gcgatggctt aatgaccacc tgaaactgat
     2821 agtagtgctg taaacggttg gggttttcgc cgtagcgacc atcggtcggg cgacgagaag
     2881 gctgaacata agcagccgcc atcggttctg gccccagcgc gcgcagacag gtcattgggt
     2941 gagaggttcc cgcgccgact tccatgtcca atggttgaac aatggtgcag ccctggcgag
     3001 cccagtaatc ctgtaaggtc aggatcaagc cctggaaggt cctggtatca aacttttgca
     3061 tattatttcg tgctggatac gtgtggattt aaaggaaggg atcagtatac ccgctggatg
     3121 gaagatatac agtacgaaac gggaaaaagc agggcttaac gcatggaaag atgcaaaaaa
     3181 tggccatctg cgtcaaaaga acagacaaac tgctctttac ggaaggtata accgcgcatt
     3241 tcatagcttc cctggaactg ttcgaaggcg gtgacgtcga ttttttgctc gccggtgtta
     3301 taacgttttg ccgcctgatc cttgcataac gcttccatat tcaacgaacg cagaggatca
     3361 acctttaccc gttgtgcttt ctgcacgggt gacggtgtag aacaacccac tagcaccata
     3421 agcgccattg ccggaaagaa agcgttcatc atcattaaat taccgtctgg tttgcaggtg
     3481 agtcttatta tttatatcgg tatagtggct gatatcttac gaatttcctt aagcctctat
     3541 aagctgtagt gataaaactc agattattcc agtgcgccaa ctgaatgatt tatcggtcag
     3601 acttttttta accagataca gagacacaga tgcagcccaa aatttactgg attgataacc
     3661 tgcgagggat agcgtgttta atggtggtga tgattcacac cactacctgg tatgtgacca
     3721 atgctcatag tgttagcccc gtcacctggg atatcgccaa tgttctgaat tctgcctctc
     3781 gtgtcagcgt gccgctattt ttcatgatat ccggctatct cttttttggc gaacgcagcg
     3841 cccagccgcg ccatttcttg cgtatcggct tatgcctgat tttttatagc gcaatcgcac
     3901 tgctctacat tgcgctgttt acctccatca atatggagtt agcgctgaaa aacctgctgc
     3961 aaaagccagt gttttaccac ttgtggtttt tcttcgcgat tgcggtgatt tatctggttt
     4021 caccgctgat tcaggtgaag aacgtcggcg gaaaaatgtt gctggtgcta atggtggtga
     4081 ttggcattat cgctaaccca aacacagtgc cgcagaaaat tgacggtttt gaatggctgc
     4141 caattaactt atatatcaat ggcgatactt tttactacat tctgtatggc atgttgggcc
     4201 gcgctatagg gatgatggac acacagcata aagcactgtc gtgggtgagc gccgcgctgt
     4261 ttgcgacggg ggtttttatt atctctcgcg ggacattata tgaattgcag tggcgcggaa
     4321 attttgccga tacctggtat ctttactgtg ggccgatggt ttttatctgc gcaatcgcgc
     4381 tattgactct ggttaaaaac acgctggata cgcgtaccat tcgcggactt ggcttaatct
     4441 cccgccattc attgggtata tacggattcc acgccttgat tatccatgcg ctgcgcaccc
     4501 ggggaattga gcttaaaaat tggccaatac tggatattat ttggatcttt tgcgcgacgt
     4561 tggcagcgag tttgttactt tctatgctgg tacaacgaat cgacagaaac agattagtga
     4621 gttaagtaaa agcccgttca cattggactg accgggctta cgtgaattat tcgctgtatt
     4681 cttcctgggt aacctgtgtt tcttttggcg ggtttattcc ggcatcacga atattcttct
     4741 gcaccgctac tgcaccaaac aagcttaaga agaaagccag tccataaaaa cctttttcac
     4801 tgagtaataa tgtcgcattc cacagaccta ccatcagtaa tgcaacagag ataataaaga
     4861 cagtcaggca ggtcatataa taaatggaag tggtcggtat gccttcatac ttgtcccgaa
     4921 cggtcttttg ataagacgca gcagaaaaca gtcctaacac cagtacggca aaataatacc
     4981 ctttttcatt taactgcatc tctgcattcc atagccctaa cagataggta acgataccac
     5041 caacgagagc tatccatgac acaatactaa aggccggtga ataggttgat attttgttgt
     5101 ccatcacagt attcctttca ttcctgaatg tgtaagagct ttatgttgct cataccgagc
     5161 gaaacaaacg catttgacca aacaagcaga caaaaaaggc gatggggtag atcaccattt
     5221 cataagcatt gataggtgca tttaaaacac cgataaacaa gagaccgata gtgattaacg
     5281 caactaactg gcagatatgg gtaaaaaaat tatccagttg cccgagtttt tctgcgcgaa
     5341 gatatgcata gttaccaaaa gttgctgtca ttaacacgcc aagaaaatag ccttttccac
     5401 ttagcaatgg gcatgagatc catagcccaa ccagataaac cagcgccccg acaacaaata
     5461 atagttttgc cacagttttt gaggttttca taacgatctc catatctacc agcgatacat
     5521 tacgagtaac caacgaaaga caaaactgaa aaatgccatt aacaaatgat tttcagaata
     5581 aattcatact aaatattaat taattagtga gatatataga tgtgaattat cccccacccg
     5641 gtcaggcagg ggataacgtt tacgccatta atggcagaag ttgctgatag aggcgacgga
     5701 acgtttcacg tcgtggctga taagcggcat aacgctgcgc atctggtaga tgcgactgtt
     5761 ctaacggtag ctgcggcaac aactcaatga gcgatttctc tggattcgcc gcaatctgcg
     5821 ccagccttgc tgcgcccagt gctggcccca catccccccc cgtacggtaa tcgagctgct
     5881 gaccgctgat atccgccagc atctgacgcc agtactcact acgcgccccg ccaccaatca
     5941 acgtaacact ttgcggttta atgccgcagg catgcacgac atccatgcca tctgccagcg
     6001 cataacccac gccttccagc actgctcgcg ccagttcatt ggggccatgt tgatgagtca
     6061 aaccaaagaa aacccccttc gcctggggat tattgtgtgg tgtacgctcg ccggaaagat
     6121 aaggcagaaa ccaaactggc tcggcacttt catcagcctg ttgagctgca gcgattaaag
     6181 ctgggacatt gcacaggccg gttaatttcg cgacccaatc cagacacgat gctgcactca
     6241 gcattacaga cattaaatgc caacgctgcg gtagcgcatg gcaaaagctg tgtacagcgc
     6301 tttctggctt gcttaagaac ccttcgctga cagcaaaata gacccccgac gtccccagcg
     6361 ataacattgc ctgattagca tcaaccattc ccacaccaac tgcaccagct gcattgtcgc
     6421 caccgcctgc gacaactggc accgtcgcca taccccacgc tttcgcaact tcaggtaaca
     6481 aagcaccagt aatttcgctg ccttcgtata atgcgggcat ctggtcacga gataagtcgc
     6541 aagcctgcag catgacgtca ctccagtcac gctttgcgac atccagccac atggtgccag
     6601 ctgcgtcaga catatcgctg gcaaactccc ccgtcatacg cagatgcaag taatctttcg
     6661 gtaataatac tttgtcgatt tgacggaata tctccggctc atgccgctga acccatagca
     6721 atttaggcgc agtaaatccg ggcatcatca ggttgccggt aatcactcgt gattgcggaa
     6781 ctctcgcttc cagcaaagtg cactcttgcg cacagcgccc gtcgttccac aaaatggcag
     6841 ggcgcaatac ccgttgttga gcatccagta aggttgctcc atgcatctgg ccggcaatac
     6901 ccaatgcttt aacgtcctgc agagaatgct gatcgcccag agctttcatt gcgcgatcag
     6961 ttgcctgcca ccactgttcc gggtcttgtt ccgaccagag tggatgcggg cgcgaaacgg
     7021 tcagcttttc cgtttgcgaa gcaaccacct caccctgctc gttgagcaaa ataactttta
     7081 cgcccgaggt gccaagatct atcccgatat acatatcgat cgttccttaa aaaaatgccc
     7141 ggtatcgcta ccgataaccg ggccaacgga ctgcacagtt agccgttatt tgtcgaacag
     7201 ataatgattt accagatttt ccagttgctc ctggcgacca ctctgatgca ccggagacaa
     7261 attatgttcc tgagcatatt tggctaaatc tgccagtgac atttggcctt tcaggatttg
     7321 ctggcccaat tcgctattcc agccggaata acgctgcgcg atgcgtttat ccagctcgcc
     7381 atcttcaatc atgcgcgctg caattttcag cgccagtgcc atcgtatcca tcgcgccgat
     7441 atgaccgtaa aacagatcat atttatcagt actttgacga cgtactttgg catcgaagtt
     7501 cagaccaccg gtggtgaaac cgcctgcttt gagaatttca tacatcacca gcgcattctc
     7561 ttccacactg ttcgggaact ggtcggtgtc ccagcccagt tgcgcatcgc cacggttggc
     7621 gtcgacagaa ccgaacaggc caagcgcaat ggcggtggct atttcatgat ggaaagagtg
     7681 acctgccagc gtcgcgtggt tagcttcaat gttcagttta atctcttttt ccagaccaaa
     7741 ctgtttcagg aagccataga ccgtcgcggc atcgtaatca tattgatgtt tagtcggttc
     7801 ttgcggtttc ggttcgataa gcaacgtgcc ctggaagccg attttatgtt tatgctcaac
     7861 caccatctgc ataaagcggc ccagttgttc acgctcctga cgcaagtcgg tatttaacag
     7921 cgtttcgtaa ccttcacgac caccccacag gacatagttt tcaccgccca atttatgggt
     7981 tgcttccatc gctgtaacaa cttgcgttgc cgcccagctg aagacttcag gatctgggtt
     8041 cgtcgccgca cccgcgccgt agcgagggtt tgtaaagcag ttggcggttc cccacagcag
     8101 cttcacgccg ctctcttctt gcttgcctgc caggacatca accatttgcg caaaattatt
     8161 gatgtactct tttaacgacg cgccctcagg ggaaacatcc acatcgtgga agcaataaaa
     8221 tggcacatgt aacttgtgga aaaactcaaa tgcgacatct gctttacgct tcgccaacgc
     8281 cagtgcctca ccaggctgct gccacggacg attaaacacc cccacaccaa acatgtccgc
     8341 cccgttccag cagaaggtgt gccagtagca ggcggcaaaa cgcaagtgct cttccatacg
     8401 cttacccaac accagttcgt cgggattgta gtgacggaat gctaacgggt ttgaggattt
     8461 tgagccttca taacgaacgc gatcgagctg gtcaaaatag gcttgcatat tgaactccat
     8521 aatcaggtaa tgccgcgggt gatggatgat gtcgtaatat tgggcactcc ctttcagttg
     8581 ctcaattatg ttatttcaca ctgctattga gataattcac aagtgtgcgc tcgctcgcaa
     8641 aataaaatgg gatgatgaaa ctgggtaatt cctcgaagat aaaaatgcaa taagtacaat
     8701 tgcgcaacaa aagtaagatc tcggtcataa atcaagaaat aaaccaaaaa tcgtaatcga
     8761 aagataaaaa tctgtaattg ttttcccctg tttagttgct aaaaattggt tacgtttatc
     8821 gcggtgattg ttacttatta aagctgtcct ctaactacag aaggccctac accatgaaaa
     8881 taaagaacat tctactcacc ctttgcacct cacttctgct taccaacgtt gcggcacacg
     8941 ccaaagaagt caaaataggt atggcgattg atgatctccg tcttgaacgc tggcaaaaag
     9001 atcgagatat ttttgtgaaa aaggcagaat ctctcggcgc gaaagtattt gtacagtctg
     9061 caaatggcaa tgaagaaaca caaatgtcgc agattgaaaa catgattaac cggggtgtcg
     9121 atgttcttgt cattattccg tataacggcc aggtattaag taacgttgta aaagaagcca
     9181 aacaagaagg tattaaagta ttagcttacg accgtatgat taacgatgcg gatatcgatt
     9241 tttatatttc tttcgataac gaaaaagtcg gtgaactgca ggcaaaagcc ctggtcgata
     9301 ttgttccgca aggtaattac ttcctgatgg gcggctcgcc ggtagataac aacgccaagc
     9361 tgttccgcgc cggacaaatg aaagtgttaa aaccttacgt tgattccgga aaaattaaag
     9421 tcgttggtga ccaatgggtt gatggctggt taccggaaaa cgcattgaaa attatggaaa
     9481 acgcgctaac cgccaataat aacaaaattg atgctgtagt tgcctcaaac gatgccaccg
     9541 caggtggggc aattcaggca ttaagcgcgc aaggtttatc agggaaagta gcaatttccg
     9601 gccaggatgc ggatctcgca ggtattaaac gtattgctgc cggtacgcaa actatgacgg
     9661 tgtataaacc tattacattg ctggcaaata ctgccgcaga aattgccgtt gaattgggca
     9721 atggtcagga gccaaaagca gatacctcac tgaataatgg cctgaaagat gtcccctccc
     9781 gcctcctgac accgatcgat gtgaataaaa acaacatcaa agatacggta attaaagacg
     9841 gattccacaa agagagcgag ctgtaagcgt tacgccccgg cgcggagcgg gggcgtgatt
     9901 tctctccatg ccgcgtgaat gaattggctt aggtggagtc gttatgcctt atctacttga
     9961 aatgaagaac attaccaaaa ccttcggcag tgtgaaggcg attgataacg tcagcttgcg
    10021 gttgaatgct ggcgaaatcg tctcactctg tggggaaaat gggtctggta aatcaacgct
    10081 gatgaaagtg ctgtgtggta tttatcccca tggctcctac gaaggcgaaa ttatttttgc
    10141 gggagaagag attcaggcga gtcacatccg cgataccgaa cgcaaaggta tcgccatcat
    10201 tcatcaggaa ttggccctgg tgaaagaatt gaccgtgctg gaaaatatct tcctgggtaa
    10261 cgaaataacc cacaatggca ttatggatta tgacctgatg acgctacgct gtcagaagct
    10321 gctcgcacag gtcagtttat ccatttcacc tgatacccgc gttggcgatt tagggcttgg
    10381 gcaacaacaa ctggttgaaa ttgccaaggc acttaataaa caggtgcgct tgttaattct
    10441 cgatgaaccg acagcctcat taactgagca ggaaacgtcg gttttactgg atattattcg
    10501 cgatctacaa cagcacggta tcgcctgtat ttatatttcg cacaaactca acgaagtcaa
    10561 agcgatttcc gatacgattt gcgttattcg cgacggtcag cacattggta cgcgtgatgc
    10621 tgccggaatg agtgaagacg atattatcac catgatggtc gggcgagagt taaccgcgct
    10681 ttaccctaat gaaccacata ccaccggaga tgaaatatta cgtattgaac atctgacggc
    10741 atggcatccg gttaatcgtc atattaaacg agttaatgat gtctcgtttt ccctgaaacg
    10801 tggcgaaata ctgggtattg ccggactcgt tggtgccgga cgtaccgaga ccattcagtg
    10861 cctgtttggc gtgtggcccg gacaatggga aggaaaaatt tatattgatg gcaaacaggt
    10921 agatattcgt aactgtcagc aagccatcgc ccaggggatt gcgatggtac ccgaagacag
    10981 aaagcgcgac ggcatcgttc cggtaatggc ggttggtaaa aatattaccc tcgccgcact
    11041 caataaattt accggtggca ttagccagct tgatgacgcg gcagagcaaa aatgtattct
    11101 ggaatcaatt cagcaactca aagttaaaac gtcgtccccc gaccttgcta ttggacgttt
    11161 gagcggcggc aatcagcaaa aagcgatcct cgctcgctgt ctgttactta acccgcgcat
    11221 cctcattctt gatgaaccta ccaggggtat cgatattggc gcgaaatacg agatctacaa
    11281 attaattaac caactcgtcc agcagggtat tgccgttatt gtcatctctt ccgaattacc
    11341 tgaagtgctc ggccttagcg atcgggtact ggtgatgcat gaagggaaac taaaagccaa
    11401 cctgataaat cataacctga ctcaggagca ggtgatggaa gccgcattga ggagcgaaca
    11461 tcatgtcgaa aagcaatccg tctgaagtga aattggccgt accgacatcc ggtggttttt
    11521 ctgggctgaa gtcactgaat ttgcaggtct tcgtgatgat tgcagctatc atcgcaatca
    11581 tgctgttctt tacctggacc accgatggtg cctacttaag cgcccgtaac gtctccaacc
    11641 tgttacgcca gaccgcgatt accggcatcc tcgcggtagg aatggtgttc gtcataattt
    11701 ctgctgaaat cgacctttcc gtcggctcaa tgatggggct attaggtggc gtcgcggcga
    11761 tttgtgacgt ctggttaggc tggcctttgc cacttaccat cattgtgacg ttggttctgg
    11821 gactgcttct cggtgcctgg aacggatggt gggtcgcgta ccgcaaagtc ccttcattta
    11881 ttgtcaccct cgcgggcatg ttggcatttc gcggcatact cattggcatc accaacggca
    11941 cgactgtttc ccccaccagc gccgcgatgt cacaaattgg gcaaagctat ctacctgcca
    12001 gcactggctt catcattggc gcgcttggct taatggcttt tgttggttgg caatggcgcg
    12061 gaagaatgcg ccgtcaggct ttgggtttgc agtctccggc ctctaccgca gtagtcggtc
    12121 gccaggcttt aaccgctatc atcgtattag gcgcaatctg gctgttgaat gattaccgtg
    12181 gcgttcccac tcctgttctg ctgctgacgt tgctgttact cggcggaatg tttatggcaa
    12241 cgcggacggc atttggacga cgcatttatg ccatcggcgg caatctggaa gcagcacgtc
    12301 tctccgggat taacgttgaa cgcaccaaac ttgccgtgtt cgcgattaac ggattaatgg
    12361 tagccatcgc cggattaatc cttagttctc gacttggcgc tggttcacct tctgcgggaa
    12421 atatcgccga actggacgca attgcagcat gcgtgattgg cggcaccagc ctggctggcg
    12481 gagtgggaag cgttgccgga gcagtaatgg gggcatttat catggcttca ctggataacg
    12541 gcatgagtat gatggatgta ccgaccttct ggcagtatat cgttaaaggt gcgattctgt
    12601 tgctggcagt atggatggac tccgcaacca aacgccgttc ttgattttga taaaaatttt
    12661 ctcaaagccg gttacgtatt accggttttg agtttttgca tgattcagca ggaaaagaac
    12721 catgtttact aaacgtcacc gcatcacatt actgttcaat gccaataaag cctatgaccg
    12781 gcaggtagta gaaggcgtag gggaatattt acaggcgtca caatcggaat gggatatttt
    12841 cattgaagaa gatttccgcg cccgtattga taaaatcaag gactggttag gagatggcgt
    12901 cattgccgac ttcgacgaca aacagatcga gcaagcgctg gctgatgtcg acgtccccat
    12961 tgttggggtt ggcggctcgt atcaccttgc agaaagttac ccacccgttc attacattgc
    13021 caccgataac tatgcgctgg ttgaaagcgc atttttgcat ttaaaagaga aaggcgttaa
    13081 ccgctttgct ttttatggtc ttccggaatc aagcggcaaa cgttgggcca ctgagcgcga
    13141 atatgcattt cgtcagcttg tcgctgaaga aaagtatcgc ggagtggttt atcaggggtt
    13201 agaaaccgcg ccagagaact ggcaacacgc gcaaaatcgg ctggcagact ggctacaaac
    13261 gctgccaccg caaaccggga ttattgccgt tactgacgcc cgagcgcggc atattctgca
    13321 agtatgtgaa catctacata ttcccgtacc ggaaaaatta tgcgtgattg gcatcgataa
    13381 cgaagaactg acccgctatc tgtcgcgtgt cgccctttct tcggtcgctc agggcgcgcg
    13441 gcaaatgggc tatcaggcgg caaaactgtt gcatcgatta ttagataaag aagaaatgcc
    13501 gctacagcga attttggtcc caccagttcg cgtcattgaa cggcgctcaa cagattatcg
    13561 ctcgctgacc gatcccgccg ttattcaggc catgcattac attcgtaatc acgcctgtaa
    13621 agggattaaa gtggatcagg tactggatgc ggtcgggatc tcgcgctcca atcttgagaa
    13681 gcgttttaaa gaagaggtgg gtgaaaccat ccatgccatg attcatgccg agaagctgga
    13741 gaaagcgcgc agtctgctga tttcaaccac cttgtcgatc aatgagatat cgcaaatgtg
    13801 cggttatcca tcgctgcaat atttctactc tgtttttaaa aaagcatatg acacaacgcc
    13861 aaaagagtat cgcgatgtaa atagcgaggt catgttgtag cgatgatgag aattgtcggc
    13921 gtcacatcag ggaactcagc acagccacct gatgcgagca aaagccttat ccgaccgaca
    13981 aatcagataa ggctcaagga aatgcaatta catatgcgcc gcgattaacc gttggttatc
    14041 ctggtacatt gcgaacaggt agttattata actcttccct ttggtcgagt agcccttcag
    14101 cttgtgaatc atcgctgtgg cagtcacttc ctgatccgct ttacgcagct gcgcacgcga
    14161 tttacggaac gaagagtaag ccgggtgcgt attcaggtta gtgacatagg cgctcaccga
    14221 ttctttgaca gaactaaact gtgagtaccc tttcacttta cccggcgcat tggtacaacg
    14281 tcctttcatg catttcatgc cgaacagatt gttattgttg cgcgccagct tcgacgttcc
    14341 ccaaccgctt tctgctgcag ccatcgtcgc caccatactg gtagggataa tgtctacgcg
    14401 ttcaagcaag gtattccacg ggatttttcg cgtattacca gaccacttca ccttgtagcg
    14461 tttggcgatg tctttcagac gcgcacgctc agcaggtgac cattggccct gatactgttt
    14521 tgaaatcagc cagttacgtt ctgcagtaat ggctgcattt tggctggtaa tgtaaggcat
    14581 tacggtccgg agaaacgcct ttttccttgg tgttccggaa gggtattttc gcaaatcagg
    14641 aagtgaacta ctctttgcac tattgcgaga atactcttgt ttactgctta cctgtttatt
    14701 actagcttta gttaagtggg acttttgact cgctgttgtt gtgtgcgtct tcgctaacac
    14761 ctcactcgaa aacaccagag tgagtaacat aagaatcatc gccccatatc gtcgtatggg
    14821 agtcaaaatc atcaggtctc ctggtcggat ttaatcattc caacacctta tatttttcac
    14881 aaatttgaga gttgaatctc aaatcatatc aaaaatagct gtcaagagca ccccaaggaa
    14941 tagtccaaat ctgaaactat gtcacgtgtt aacgattcag attggcgcta aatcgcagaa
    15001 aatgtggggt ttatcgcaaa attcagccgt tttttgcgcg agatcgctca cccttgcttc
    15061 tcatcctgtg gacttaccgc tcagggatga gttttgtctg gcttatcgct ggcaaactgt
    15121 ctgaaatcgc agcaataagg actcatccac catgaaactc gccgcctgtt ttctgacact
    15181 ccttcctggc ttcgccgttg ccgccagctg gacttctccg gggttccctg cctttagcga
    15241 acagggaacg ggaacatttg tcagccacgc gcagttgccc aaaggtacgc gtccactcac
    15301 gctaaatttt gaccaacagt gctggcagcc tgcagatgcg ataaaactca atcagatgct
    15361 ttccctgcaa ccttgtagca acacgccgcc tcaatggcga ttgttcagag acggcaaata
    15421 tacgctgcaa atagacaccc gctccggtac gccaacattg atgatttcca tccagaacgc
    15481 cgccgaaccg gtagcaaacc tggtccgtga atgcccgaaa tgggatggat taccgctcac
    15541 gctggatgtc agcgccactt tcccggaagg agccgccgta cgggattatt acagccagca
    15601 aattgcgata gtgaagaacg gtcaaataac gttacaaccc gctgctacca gcaacggttt
    15661 actcctgctg gaacgggcag aaactgacgc ccctgcccct ttcgactggc ataacgccac
    15721 ggtttacttt gtgctgacag atcgtttcga aaacggcgat cccagtaatg accagagtta
    15781 cggacgtcat aaagacggta tggcggaaat tggcactttt cacggcggcg atttacgcgg
    15841 cctgaccaac aaactggatt acctccagca gttgggcgtt aatgctttat ggataagcgc
    15901 cccatttgag caaattcacg gctgggtcgg cggcggtaca aaaggcgatt tcccgcatta
    15961 tgcctaccac ggttattaca cacaggactg gacgaatctt gatgccaata tgggcaacga
    16021 agccgatcta cggacgctgg ttgatagcgc acatcagcgc ggtattcgta ttctctttga
    16081 tgtcgtgatg aaccacaccg gctatgccac gctggcggat atgcaggagt atcagtttgg
    16141 cgcgttatat ctttctggtg acgaggtgaa aaaaacgctg ggtgaacgct ggagcgactg
    16201 gaaacctgcc gccgggcaaa cctggcatag ctttaacgat tacattaatt tcagcgacaa
    16261 aacaggctgg gataaatggt ggggaaaaaa ctggatcaga acggatatcg gcgattacga
    16321 caatcctgga ttcgacgatc tcactatgtc gctggccttt ttgccggata tcaaaaccga
    16381 atcaactacc gcttctggtc tgccggtgtt ctataaaaac aaaacggata ctcacgctaa
    16441 agtcatcgaa ggctttacac ctcgcgatta cttaacccac tggttaagtc agtgggtccg
    16501 cgactatggg attgatggtt ttcgggtcga taccgccaaa catgttgagt tgcccgcctg
    16561 gcagcaactg aaaaccgaag ccagcgccgc gcttcgcgaa tggaaaaaag ctaaccccga
    16621 caaagcatta gatgacaaac ctttctggat gaccggtgaa gcctggggcc acggcgtgat
    16681 gcaaagtgac tactatcgcc acggcttcga tgcgatgatc aatttcgatt atcaggagca
    16741 ggcggcgaaa gcagtcgatt gtctggcgca gatggatacg acctggcagc aaatggcgga
    16801 gaaattgcag ggtttcaacg tgttgagcta cctctcgtcg catgataccc gtctgttccg
    16861 tgaagggggc gacaaagcag cagagttatt actattagcg ccaggcgcgg tacaaatctt
    16921 ttatggcgat gaatcctcgc gtccgttcgg tcctacaggt tctgatccgc tgcaaggtac
    16981 acgttcggat atgaactggc aggatgttag cggtaaatct gccgccaacg tcgcgcactg
    17041 gcagaaaatc agccagttcc gcgcccgcca tcccgcaatt ggcgcgggca aacaaacgac
    17101 actttcgctg aagcagggct acggctttgt tcgtgagcat ggcgacgata aagtgctggt
    17161 catctgggct gggcaacagt gacttttccg gcttcccgtt cgtcagtacc tcgggaagcc
    17221 gccaaccagg ataaaatgtc agccctaatc agcgttgcag gataaagcac cgctcactct
    17281 ccaacagacc gatttgcacc ccagcaaatg tagcgttatt gttaccttcc ttgctacaga
    17341 gttcgacaga tatcccgcta tgacattctc cctttttggt gacaaattta cccgccactc
    17401 cggcattacg ctgctgatgg aagatctgaa cgacggttta cgcacgcctg gcgcgattat
    17461 gctcggcggc ggtaatccgg cgcagatccc ggaaatgcag gactacttcc agacgctact
    17521 gaccgacatg ctggaaagtg gcaaagcgac tgatgcactg tgcaattacg acggtccaca
    17581 ggggaaaacg gagctactca cactgcttgc cggaatgctg cgcgagaagt tgggttggga
    17641 tatcgaagca cagaatattg cactaacaaa cggcagccag agcgcgtttt tctacttatt
    17701 taatctgttt gccggacgcc gtgccgatgg tcgggtcaaa aaagtgctgt tcccgctggc
    17761 accggaatac attggctacg ctgacgccgg actggaagaa gatctgtttg tctctgcgcg
    17821 tccgaatatt gaactgctgc cggaaggcca gtttaaatac cacgtcgatt ttgagcatct
    17881 gcatattggc gaagaaaccg ggatgatttg cgtctcccgg ccgacgaatc caacaggcaa
    17941 tgtgattact gacgaagagt tgctgaagct tgacgcgctg gcgaatcaac acggcattcc
    18001 gctggtgatt gataacgctt atggcgtccc gttcccgggt atcatcttca gtgaagcgcg
    18061 cccgctatgg aatccgaata tcgtgctgtg catgagtctt tccaagctgg ggctgcctgg
    18121 ctcccgctgc ggcattatca tcgccaatga aaaaatcatc actgccatca ccaatatgaa
    18181 cggcattatc agcctggcac ctggcggtat tggtccggcg atgatgtgtg aaatgattaa
    18241 gcgtaacgat ctgctgcgcc tgtctgaaac agtcatcaaa ccgttttact accagcgtgt
    18301 tcaggaaact atcgccatca ttcgccgcta tttaccggaa gatcgctgcc taattcataa
    18361 accagaagga gccattttcc tctggctatg gtttaaggat ttgcccatta cgaccgagca
    18421 actctaccag cgcctgaaag cacgcggcgt gctgatggtg ccggggcaca acttcttccc
    18481 gggactggat aaaccgtggc cgcatacgca tcaatgtatg cgcatgaact acgtaccaga
    18541 gccggagaaa attgaggcgg gggtgaagat tctggcggaa gagatagaaa gagcctgggc
    18601 tgaaagtcac taaaaattgc ctgatacgct acgcttatca ggcctacata atttctgcaa
    18661 tatattgaat ttggaagaat ttgtaggccg gataaggcgt ttacgccgca tctggcataa
    18721 acaaagcgca ctattccggc gttgagaaac gccggaaaac gtttcaaaca ggctgctgcc
    18781 gtagccgatg ccgctgcact ttctcgacat ccatgcactg caacgccttt gtcgggcagg
    18841 cttcaacaca ggccggcccc gtctcccgat gccagcacag atcgcactta atcgccctcg
    18901 cctttttgcg cgaagaaacg acctccatcg caccaaacgg gcaagccagc atacagcttt
    18961 tacagccaat gcaacgtgtt tgttcaacga aaatatgccc atgttcgcgg cttatcgcgt
    19021 taacagggca gacattcgcg cacggtgcat cttcacactg atgacaggct actgccgtgg
    19081 tccagctgtg gtctttaatg acacgaatac gggaaataaa ctcgtctggt gacaacgcag
    19141 cgcaatcctg attctcctga tgcgacactg cgcaagccac ttcacaggta cggcaaccga
    19201 tacatttcgt cgcatccgca ataataaacc ggttcatgat cactcctttg cactaaaaca
    19261 gcagagtgcc agagagcgcg tccctactgc tttgatccaa cgaaggaaat gttaaagttt
    19321 tggcacttgt ccaaagcgtt atgtgatcgc gccctggtcg tccctgacag taaatcccag
    19381 ttcattagaa atagcctgcg cggtttcacg cagtggtttc aggagatttt tctcccccac
    19441 ctgtttcaga cgtgatgtcg aaagcgaaat cgacacggcg tacggcaccc gcccatgaat
    19501 attaaacacc ggaacagcaa tacaggagac gccgagttcg ttttcttctc tgtccatcgc
    19561 cgctccgctt tcacgaatgt gcgccagttc gtcgaacatc gcgggcagct cggtaatggt
    19621 attgcgggtt aacggctgga tctcatgctg atggttttcc cagtatgact tcacgtagtc
    19681 cgggtgacca aacgccatgt agatcttgcc cattgcggaa cagtagagcg gcatatgctg
    19741 gccaatatag gcacgggttc gcagcatccc ggttgtgggt tccagcttat aaatcaaaat
    19801 agcgtgatcg tcttcgcggc tggagaagtt aatggtttca ccagtggcga tgttcagtgc
    19861 ctcaagatgc ggagcggcga tatgaatgat attcagcgaa gacagcgcct tctgcccgac
    19921 ggcaataaat ttggtagtca ggcggtaact ccctgcggcg ggcgcggtgg tcacatagcc
    19981 gcaggactgt aatccctgca ataagcgatg gacggtactc ttatttaaac cagccagctc
    20041 cgaaagatgc gccaacggac aaccgtttgg atagttgctc aaaatctcaa tcagcatcaa
    20101 cccgcgaaac agactctggc ttccggctgg acgctctttt tcctgcgcca tctcgttctc
    20161 tttttttccc atcacttctt tccccatttt gtcgcgtcct gatggtagcg caaagtgtgc
    20221 cgtagttcac gatctcgaca gataatttat aaccacttga tttttatgtc ttttgaaatt
    20281 catcaatcag attgctttgt taaaaagtga tcgatatatt tgaaatcaag tttcgcatat
    20341 tgaaatttta agccaaaaaa gcgatcaaaa aaacaaggaa gcctgggatg aaagtgacat
    20401 ttgagcagtt aaaagcagcc tttaatcggg tcttaatttc acgcggcgtt gacagcgaaa
    20461 cggctgacgc ctgtgcagag atgttcgccc gcaccaccga atccggcgtt tattctcacg
    20521 gcgttaatcg tttccctcgt ttcattcaac aactggaaaa cggcgatatc attcctgatg
    20581 cccaacccaa acgtataacc agcctcggcg caattgaaca gtgggatgcc cagcgttcga
    20641 tcggcaacct gacggcgaaa aagatgatgg atcgcgccat tgaactggct gccgatcacg
    20701 gtattggtct ggtggcactt cgtaatgcca accactggat gcgcggcggc agctacggct
    20761 ggcaggcggc ggaaaaaggc tatattggca tttgctggac caactccatc gccgtaatgc
    20821 cgccgtgggg cgcaaaagag tgtcgcattg gcactaaccc gctgatcgtc gccattcctt
    20881 ccacgccgat caccatggtc gatatgtcga tgtcgatgtt ctcttacggc atgttagaag
    20941 ttaaccgcct ggcgggccgt cagctcccgg tcgatggtgg ctttgatgat gagggcaatt
    21001 tgaccaaaga acctggcgtt atcgagaaga atcgccgcat tttgccgatg ggctactgga
    21061 aaggttctgg catgtcgatt gtgctggata tgatcgctac tctcctttcc gacggcgcat
    21121 cggttgccga agtcacccag gacaacagcg acgaatacgg catttcacaa atttttattg
    21181 ccattgaagt ggacaagctt atcgacggtc ccacccgcga tgccaagctg caacgtatca
    21241 tggattacgt tactaccgct gaacgcgctg acgaaaacca ggccatccgc ttacccggcc
    21301 acgaatttac taccctgctg gccgaaaacc gccgtaacgg tattaccgtt gatgacagcg
    21361 tgtgggccaa aatccaggcg ttataaggag ataagtcatg atttttggac atatcgcgca
    21421 gcccaatccg tgccgtttgc ccgccgccat tgaaaaggcg ctcgattttc tgcgcgctac
    21481 cgatttcaac gccctggagc cgggcgttgt cgaaatcgac ggcaaaaata tttatgcgca
    21541 aattatcgat ttaaccacac gcgaagcggt ggaaaaccgt ccggaagtgc atcgtcggta
    21601 tatcgatatt cagtttctgg cgtggggtga agagaaaatc ggcattgcta ttgatacggg
    21661 aaataataaa gtcagcgaat cactattaga gcagcgcgat attatttttt atcatgacag
    21721 tgaacataaa tcatttatag aaatgatacc gggcagctac gcaattttct ttccgcagga
    21781 tgttcaccga ccaggttgta ttttgcaaac agcctctgag atacgaaaaa ttgtggttaa
    21841 agtcgcatta acggcgctga attaatcgaa taatcgtcta cgccagaacg actgggtaat
    21901 gttattgctc taactatatt aataatttag gttgctgcac atgcaacttg aattatgaca
    21961 gacatagcct caaggaatag ctatgaaaaa aatacttgaa gcaatactgg cgattaatct
    22021 cgccgtactt tcctgtattg tgtttataaa cattatttta agatatggtt ttcagacaag
    22081 cattttatct gtagatgaat tgtcacgtta tttatttgtc tggttaacat ttattggcgc
    22141 gattgtggct tttatggata acgcccacgt tcaggtcacc tttctggtgg agaaactctc
    22201 ccccgcatgg caacgacgag tcgctctggt tacccattcc ttaatcttat ttatttgtgg
    22261 cgcactggcc tggggcgcaa cgctaaaaac aattcaggac tggagcgatt attcaccgat
    22321 cctcggttta cccatcggcc tgatgtatgc cgcttgtctg cccaccagcc tcgttatcgc
    22381 attctttgaa ctgcgtcatt tatatcaact catcacgcgt agcaattctc taacgtcgcc
    22441 accgcaagga gcttaatcat ggctgtgctg atttttctgg gctgtctgtt gggtggtatt
    22501 gctatcggtt tgcctattgc ctgggcactg ttgttgtgcg gggcggcgtt aatgttctgg
    22561 ctggacatgt ttgatgtcca gatcatggca caaacgctgg tgaacggagc cgatagcttc
    22621 tccctgctgg cgattccgtt ctttgtgctg gcgggtgaaa tcatgaatgc gggcggcttg
    22681 tcaaaacgca ttgttgacct gccgatgaaa ctggtggggc ataaaccagg cgggctgggc
    22741 tacgtcggcg tgctggcagc tatgattatg gcgagccttt ctggttctgc cgttgcggat
    22801 accgccgccg ttgccgctct actggtgccg atgatgcgca gcgccaacta cccggtcaac
    22861 cgggcggcgg ggctgattgc ctctggcggc attatcgcgc caattattcc cccttccatt
    22921 ccgtttatta tcttcggcgt ttccagcgga ttatccatca gtaagctgtt tatggcgggc
    22981 attgcccctg gcatgatgat gggcgcaacg ctgatgctta cctggtggtg gcaggccagt
    23041 cgccttaatt taccccgcca gcaaaaagca acgatgcagg aaatctggca ctcttttgtc
    23101 tccggtatct gggcgctgtt tcttccagtc atcattattg gcgggtttcg ctccggtctg
    23161 tttaccccta ccgaagccgg ggccgttgct gctttctatg cgctgtttgt cgccacagtt
    23221 atttaccgtg aaatgacctt cgccacactt tggcatgtac tcattggcgc ggcgaaaacc
    23281 acctcagtgg ttatgtttct ggtggcctcg gcacaagttt ccgcctggct gattaccatt
    23341 gctgaactgc cgatgatggt ttctgattta ctgcaaccgt tggtcgattc accgcgtctg
    23401 ctgtttatcg tcattatggt ggctatcctg attgtcggca tggtcatgga tttaacgccg
    23461 acggtattaa ttcttacccc ggtattaatg cctttagtta aagaggcagg aatcgatccg
    23521 atttatttcg gtgtcatgtt tatcattaac tgctcaatcg gcttaattac accgcctatc
    23581 ggtaatgtac ttaacgttat ttcaggggtg gcaaaactca aattcgatga tgcagtcaga
    23641 ggcgttttcc cttacgtcct ggttttatat tcattattag tcgtatttgt ttttattccc
    23701 gatctcatca tcctcccttt gaaatggatt aattaaaagg aaaatattat gaaattacgc
    23761 tctgtaacct acgcattatt cattgctggc ctggctgcat tcagcacatc ttctctggcg
    23821 gcgcaatctt tacgtttcgg ttatgaaacc tcacaaaccg actcgcaaca tattgcggcg
    23881 aaaaaattca atgatttatt gcaggagaga accaaaggcg agctgaaatt aaaattgttt
    23941 ccggatagca ccctcggtaa cgcgcaggcg atgatcagcg gcgtacgtgg cggcaccatc
    24001 gatatggaaa tgtccggctc gaataacttt gccgggttat caccagtgat gaacttgctt
    24061 gatgtccctt tcctgttccg cgataccgct cacgcgcata aaacgctcga cggcaaagtc
    24121 ggtgatgatc tgaaagcctc acttgaaggt aaaggactaa aagtactggc ctactgggaa
    24181 aacggctggc gcgatgtcac caactcgcgc gcaccggtta aaacccccgc cgacctgaaa
    24241 gggctgaaaa ttcgcaccaa caatagcccg atgaatatcg ccgcattcaa agtctttggc
    24301 gctaacccga tcccgatgcc gtttgccgaa gtctataccg ggctggaaac ccgcactatc
    24361 gacgctcagg aacacccgat caacgtcgtc tggtcagcaa aatttttcga agtgcagaag
    24421 tacctttctc tgacgcacca cgcctattcc ccgcttctgg tggtgatcaa caaagcgaag
    24481 tttgatggct taaccccgga gttccagcaa gcgctaattt catctgcgca ggaagcgggt
    24541 aactaccagc gcaaactggt tgccgaagat cagcaaaaaa tcatcgacgg catgaaagaa
    24601 gcgggcgtgg aagtcatcac cgatctcgac cgcaaagcct ttagcgacgc actgggtact
    24661 caggtccgcg acatgtttgt aaaagatgtt ccgcagggtg ctgatctact gaaagccgtg
    24721 gatgaggtgc aataaaccat gacgcaatac tggctggggt tagattgtgg cggtagctgg
    24781 ctgaaagccg ggctgtatga ccgcgaaggc cgggaggcag gcgtgcagcg cctgccgctg
    24841 tgcgcattaa gcccgcagcc aggttgggca gagcgcgata tggcagaact gtggcaatgc
    24901 tgcatggctg tcattcgcga actgcttacc cattccggcg ttagcgggga acaaattgtc
    24961 ggtatcggta tctccgcaca aggaaaaggc ttgtttttgc tggataaaaa caacaagccg
    25021 ctcggtaacg ccattttgtc ctcggatcgc cgggcgatgg aaatcgttcg tcgctggcag
    25081 gaagatggca tcccggaaaa actctacccg ttgacccgac aaaccttgtg gaccgggcat
    25141 ccggtgtcgc tgttacgctg gctgaaagag cacgaaccgg aacgctacgc gcaaattggc
    25201 tgcgtgatga tgacgcacga ctacctgcgc tggtgtttaa ccggcgtcaa aggctgtgaa
    25261 gagagcaata tttccgagtc caacctctac aacatgagtc ttggggaata tgacccgtgc
    25321 ctcaccgact ggctggggat tgctgaaatc aatcacgccc tgccgcctgt tgtcggatct
    25381 gccgaaatct gcggggagat caccgctcag acagccgtac tgaccggtct gaaagcgggt
    25441 acgcccgttg tcggcggcct gtttgatgtg gtttccaccg cactctgcgc cgggatcgaa
    25501 gacgaattta cccttaatgc ggtgatgggc acctgggcgg tgaccagcgg cataacccgc
    25561 ggtttacgtg acggtgaagc gcatccgtat gtctatggtc gctacgttaa cgatggtgaa
    25621 tttatcgttc acgaagccag ccccacctct tccggcaacc tcgaatggtt taccgcacag
    25681 tggggagaaa tctcgtttga tgagatcaac caggccgttg ccagcttgcc gaaggccggt
    25741 ggcgatctct ttttcctgcc gttcctgtac ggcagcaacg ccggactcga gatgaccagc
    25801 ggtttctacg ggatgcaggc cattcacacc cgcgcacacc tgttgcaggc catttatgaa
    25861 ggggtggtgt tcagccatat gacccatctc aaccggatgc gcgaacgttt tactgatgtg
    25921 cacaccctgc gcgtcactgg cggcccggcg cattccgatg tctggatgca aatgctggcg
    25981 gacgtcagcg gtctgcgtat tgagctgccg caggtggaag aaaccggctg ctttggtgca
    26041 gcacttgccg cccgcgtcgg caccggggtt tatcgcgatt tcagcgaagc ccagcgtgat
    26101 ttacagcacc cggtgcgcac cctgctgccg gatatgactg tccatcagct ttaccaacaa
    26161 aaataccaac gctatcagca tctcattgcc gcacttcagg gctttcacgc ccgcatcaag
    26221 gagcacatat tatgagccga ccacttctgc aactggcact cgaccactca tcacttgaag
    26281 ccgcacagcg cgacgtgacg cagttaaaag acagcgtcga tattgtcgaa gcgggcacca
    26341 ttctctgttt aaacgaaggg cttggcgcgg tgaaagcctt gcgcgaacag tgcccggaca
    26401 aaatcatcgt tgctgactgg aaggtcgccg acgctggtga aacgctcgcg caacaggcgt
    26461 ttggcgcagg cgctaactgg atgaccatca tctgcgccgc gccgctcgcg acggtagaaa
    26521 aaggccacgc aatggcacaa cgctgcgggg gtgaaattca gatagagctg ttcggtaact
    26581 ggacgctgga cgacgcccgc gactggcatc gtattggcgt gcggcaggcc atttatcatc
    26641 gcggtcgtga tgcacaggcc agcgggcaac agtggggcga agccgatctg gcacgcatga
    26701 aggcgctttc agatatcggc cttgagcttt ccattactgg cgggattacc cctgctgacc
    26761 tgccgctgtt taaagatatc cgcgtgaaag cgtttattgc cgggcgggca ctggcaggcg
    26821 cggcaaatcc ggcgcaagtg gctggcgatt tccatgcgca aatcgacgct atctggggag
    26881 gtgcgcgtgc gtaatcatcc gttagggatt tatgaaaaag cgctggcgaa agatctctcc
    26941 tggccggagc ggctggtgct ggcaaaaagc tgcggttttg attttgtcga aatgtcggtg
    27001 gatgaaaccg acgaacggct ctcacgtctt gactggagcg ccgcacaaag gacttcgctg
    27061 gttgccgcga tgatcgaaac aggtgttggg atcccgtcga tgtgcctgtc cgcacatcgt
    27121 cgctttccct ttggtagccg tgacgaggca gtgcgcgaac gggcgcggga aatcatgagt
    27181 aaagcgattc gcctggcgcg cgatctcggc attcgcacca tccagctggc aggttatgac
    27241 gtctattacg aagaccacga cgaaggcacc cggcaacgtt ttgctgaagg gctggcgtgg
    27301 gcagtcgaac aggcggcggc atcacaagta atgctggcgg tggagattat ggataccgcg
    27361 tttatgaact ccatcagcaa atggaaaaaa tgggacgaga tgctcgcctc gccgtggttc
    27421 accgtctacc cggacgtcgg caacctcagc gcctggggca atgatgttcc cgccgaactg
    27481 aaactgggca ttgaccgtat cgccgcgatc cacctgaaag acacccagcc agtcaccggg
    27541 caaagccccg gacagttccg tgatgtgccg tttggcgaag gctgcgtcga tttcgttggc
    27601 atcttcaaaa cgctgcatga actgaactat cgcggttctt tcctgattga gatgtggacc
    27661 gaaaaagcca aagagccggt gctggagatc attcaggcgc ggcgctggat tgaagcgcgt
    27721 atgcaggagg ctggatttat atgttagagc aactgaaagc cgacgtgctg gcggcgaatc
    27781 tggcgcttcc cgctcaccat ctggtgacgt tcacctgggg caatgtcagc gcggtagacg
    27841 acacgcggca atggatggta atcaaacctt ccggcgtcga gtacgacgtg atgaccgccg
    27901 acgatatggt ggtggttgag atagccagcg gcaaggtggt ggaaggtagc aaaaagccct
    27961 cgtccgatac gccaacgcat ctggcacttt accgtcgcta tgccgaaatt ggtggcattg
    28021 tgcataccca ctcgcgccac gccaccatct ggtcacaggc cgggctggat ctccccgcct
    28081 ggggcaccac ccacgccgat tatttttacg gtgctatccc ctgcacgcga cagatgaccg
    28141 cagaggagat taacggcgaa tatgaatatc agaccggcga agtgatcatt gaaaccttcg
    28201 aagaacgtgg caggagtccg gcacaaatcc cggcggtgct ggtgcattct cacggcccgt
    28261 tcgcatgggg taaaaacgcc gccgatgccg tgcataacgc cgtagtactc gaagaatgcg
    28321 cctatatggg tctattctcg cgccagcttg cgccgcagct ccctgcgatg caaaacgaac
    28381 tgctggataa gcactacctg cgtaagcatg gggccaatgc ctattacggg cagtaatccc
    28441 tcacgccggg gcttcatcgc cccggcacta cgaattgata tgttccttgc tgtaacgccg
    28501 cttccacgct gctggcgtta aaccagtatg tttctgaaaa atctgccgga aatagccaac
    28561 gtcattaaac cgtattgcat ggagcaggcc gacaacgtcg agtcactgca taacctgttg
    28621 ctgcccgccg atgcgccatt tacgacattt gaaggcaagg gattgttcag ccataagatc
    28681 ctgattcaga cgccaggttc ccggtatgaa cagagcaatc cagagcaaca acctttgtgg
    28741 cattacgacg gagcaccagc cgcaccgaca tccaccggtg aattaaaaac gccagctcac
    28801 cccggtgaca aaggtaaaac tctcattacg atcaatcatc gggctgtcgg taatctcttc
    28861 cggcagctcg ctgtatcctg cgctcgccat caatacgacg tgctctccta tcgggtattt
    28921 tgccgtcagg ctgacatagg gcacccaggc atcctgcgcg gaataacttg ccagaccgct
    28981 acgacgggac tcactctctg aaatgccata gtaatagtca ctgaaattct cgtcataata
    29041 gagaacgccc agcgcaggtg tcagcgacag gcgacctatc tgcattttgt ggaataccga
    29101 tagctccccc acccagccgt tgctgttatc cagaacgtcc gcagccgcag aggctttcac
    29161 ggttccccaa cgctcgtggt gataccaggc aacccccgcc atagccgtac tatcacgctt
    29221 atcaagctgt tgcatggcat aatcgtcatt atcggcaggg tcaaattcca gcggcatcca
    29281 ggatgcggtc aggctaagtt catttttttc actttgcgac agaataaaac ctaacgtggt
    29341 ctgacgaaca taaaaagaat caccttcata actaatcagc ggaattgcct ttgtattttt
    29401 attataaccg cgataaggcg attcattata agccgcacca gcaccaatgg ataattcaga
    29461 agcagttgcg cttgccataa aaggcaacgc aaataacgcc acaatattgc ggttaattaa
    29521 catgtccggt attccatttt aaaataagat aaaaaagagt cggcatagta atg
//
LOCUS       DAJOWB010000050        29149 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJOWB010000050 DAJOWB010000000
VERSION     DAJOWB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.050"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(444..707)
                     /gene="cedA"
                     /locus_tag="OHO85_002390"
     CDS             complement(444..707)
                     /gene="cedA"
                     /locus_tag="OHO85_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCQ2671752.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            890..3151
                     /gene="katE"
                     /locus_tag="OHO85_002391"
     CDS             890..3151
                     /gene="katE"
                     /locus_tag="OHO85_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCQ2671753.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKIADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3198..3956)
                     /gene="chbG"
                     /locus_tag="OHO85_002392"
     CDS             complement(3198..3956)
                     /gene="chbG"
                     /locus_tag="OHO85_002392"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCQ2671754.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3969..5321)
                     /gene="celF"
                     /locus_tag="OHO85_002393"
                     /gene_synonym="chbF"
     CDS             complement(3969..5321)
                     /gene="celF"
                     /locus_tag="OHO85_002393"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCQ2671755.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5426..6268)
                     /gene="chbR"
                     /locus_tag="OHO85_002394"
     CDS             complement(5426..6268)
                     /gene="chbR"
                     /locus_tag="OHO85_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCQ2671756.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6276..6626)
                     /gene="chbA"
                     /locus_tag="OHO85_002395"
     CDS             complement(6276..6626)
                     /gene="chbA"
                     /locus_tag="OHO85_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCQ2671757.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKIKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6677..8035)
                     /gene="chbC"
                     /locus_tag="OHO85_002396"
     CDS             complement(6677..8035)
                     /gene="chbC"
                     /locus_tag="OHO85_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCQ2671758.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(8120..8440)
                     /gene="chbB"
                     /locus_tag="OHO85_002397"
     CDS             complement(8120..8440)
                     /gene="chbB"
                     /locus_tag="OHO85_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCQ2671759.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8739..9077)
                     /gene="osmE"
                     /locus_tag="OHO85_002398"
     CDS             complement(8739..9077)
                     /gene="osmE"
                     /locus_tag="OHO85_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCQ2671760.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9279..10106
                     /gene="nadE"
                     /locus_tag="OHO85_002399"
     CDS             9279..10106
                     /gene="nadE"
                     /locus_tag="OHO85_002399"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCQ2671761.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLRTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10336..11223
                     /gene="cho"
                     /locus_tag="OHO85_002400"
     CDS             10336..11223
                     /gene="cho"
                     /locus_tag="OHO85_002400"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCQ2671762.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYTKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(11183..11758)
                     /gene="ves"
                     /locus_tag="OHO85_002401"
     CDS             complement(11183..11758)
                     /gene="ves"
                     /locus_tag="OHO85_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCQ2671763.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11961..12446)
                     /gene="spy"
                     /locus_tag="OHO85_002402"
     CDS             complement(11961..12446)
                     /gene="spy"
                     /locus_tag="OHO85_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCQ2671764.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12776..13744)
                     /gene="astE"
                     /locus_tag="OHO85_002403"
     CDS             complement(12776..13744)
                     /gene="astE"
                     /locus_tag="OHO85_002403"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCQ2671765.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13737..15080)
                     /gene="astB"
                     /locus_tag="OHO85_002404"
     CDS             complement(13737..15080)
                     /gene="astB"
                     /locus_tag="OHO85_002404"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCQ2671766.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(15077..16555)
                     /gene="astD"
                     /locus_tag="OHO85_002405"
     CDS             complement(15077..16555)
                     /gene="astD"
                     /locus_tag="OHO85_002405"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCQ2671767.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHARTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16552..17586)
                     /gene="astA"
                     /locus_tag="OHO85_002406"
     CDS             complement(16552..17586)
                     /gene="astA"
                     /locus_tag="OHO85_002406"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCQ2671768.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELRKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17583..18803)
                     /gene="astC"
                     /locus_tag="OHO85_002407"
     CDS             complement(17583..18803)
                     /gene="astC"
                     /locus_tag="OHO85_002407"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCQ2671769.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTSNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQSSQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            19249..20055
                     /gene="xthA"
                     /locus_tag="OHO85_002408"
     CDS             19249..20055
                     /gene="xthA"
                     /locus_tag="OHO85_002408"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCQ2671770.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            20222..20932
                     /locus_tag="OHO85_002409"
     CDS             20222..20932
                     /locus_tag="OHO85_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCQ2671771.1"
                     /translation="MNAERKFLLACLIFALVIYAIHTFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            20937..21614
                     /gene="ydjY"
                     /locus_tag="OHO85_002410"
     CDS             20937..21614
                     /gene="ydjY"
                     /locus_tag="OHO85_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCQ2671772.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            21629..22336
                     /locus_tag="OHO85_002411"
     CDS             21629..22336
                     /locus_tag="OHO85_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCQ2671773.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            22336..22884
                     /locus_tag="OHO85_002412"
     CDS             22336..22884
                     /locus_tag="OHO85_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCQ2671774.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22894..24060
                     /locus_tag="OHO85_002413"
     CDS             22894..24060
                     /locus_tag="OHO85_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCQ2671775.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEAKLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            24033..25568
                     /locus_tag="OHO85_002414"
     CDS             24033..25568
                     /locus_tag="OHO85_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCQ2671776.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            25568..26221
                     /locus_tag="OHO85_002415"
     CDS             25568..26221
                     /locus_tag="OHO85_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCQ2671777.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            26288..27595
                     /gene="ynjE"
                     /locus_tag="OHO85_002416"
     CDS             26288..27595
                     /gene="ynjE"
                     /locus_tag="OHO85_002416"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCQ2671778.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(27604..28224)
                     /locus_tag="OHO85_002417"
     CDS             complement(27604..28224)
                     /locus_tag="OHO85_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCQ2671779.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            28311..28718
                     /gene="nudG"
                     /locus_tag="OHO85_002418"
     CDS             28311..28718
                     /gene="nudG"
                     /locus_tag="OHO85_002418"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCQ2671780.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLESFMALRAARPAD"
     gene            complement(28684..28956)
                     /locus_tag="OHO85_002419"
     CDS             complement(28684..28956)
                     /locus_tag="OHO85_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCQ2671781.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
BASE COUNT         7200 a         7718 c         7155 g         7076 t
ORIGIN      
        1 gactctgcta tttcatcgca gtcacattga cccgttgata taaagaaagt gagtgccgcc
       61 atggtagact caagcctttc tttaaaagag tcgaaagcca ggcttttaat atttaaatca
      121 ccataattac tctgtattaa gtttgtagaa aacatctccc gccccatatt gttaacaaaa
      181 ttattatctc atttaaatct aagtcactta caatataagt ttaagagcga cgccacagga
      241 tgaactatca aaaatagctc atcatgatta gcaaaactta accattttaa aataaataaa
      301 caattaaaga aataagatca cttatttata gcaatagctc gtcaaaggca gctttttgtt
      361 acaggtggtt tgaatgaatg tagcaacgaa atgcagaatt tcaggtcatg taactcccgg
      421 caaaaccggg aggtatgtaa tccttactca gtcacttccc cttcctggcg gatctgattt
      481 gcccaacgtt gggcagattc aggcacagta aacgccggtg agcgcagaaa tgactctccc
      541 atcagtacaa acgcaacata tttgccacgc agcatccaga catcacgaaa cgaatccatc
      601 tttatcgcat gttctggcgg cgcgggttcc gtgcgtggga catagctaat aatctggcgg
      661 ttttgctggc ggagcggttt cttcattact ggcttcacta aacgcatatt aaaaattaga
      721 aaaactgtag tttagccgat ttagcccctg tacgtcccgc tttgcgtgta tttcataaca
      781 ccgtttccag aatagtctcc gaagcgggat ctggctggtg gtctatagtt agagagtttt
      841 ttgaccaaaa cagcggccct ttcagtaata aattaaggag acgagttcaa tgtcgcaaca
      901 taacgaaaag aacccacatc agcaccagtc accactacac gattccagcg aagcgaaacc
      961 ggggatggac tcactggcac ctgaggacgg atctcatcgt ccagcggctg aaccaacacc
     1021 gccaggtgca caacctaccg ccccagggag cctgaaagcc ccagatacgc gtaacgaaaa
     1081 acttaattct ctggaagacg tacgcaaagg cagtgaaaat tatgcgctga ccacaaatca
     1141 gggcgtgcgc atcgccgacg atcaaaactc actgcgtgcc ggtagccgtg gtccaacgct
     1201 gctggaagat tttattctgc gcgagaaaat cacccacttt gaccatgagc gcatcccgga
     1261 acgtattgtt catgcacgcg gatcagcagc tcacggttat ttccagccat ataaaagctt
     1321 aagcgatatc accaaagcgg atttcctctc agatccgaac aaaatcaccc cagtatttgt
     1381 acgtttctct accgttcagg gtggcgctgg ctctgccgat accgtgcgtg atatccgtgg
     1441 ctttgccacc aagttttata ctgaagaggg tatttttgac cttgttggca ataacacgcc
     1501 aatcttcttt atccaggatg cgcataaatt ccccgatttt gttcatgcgg taaaaccaga
     1561 accgcactgg gcaattccac aagggcaaag tgcccacgat actttctggg attatgtttc
     1621 tctgcaacct gaaactctgc acaacgtgat gtgggcgatg tcggatcgcg gcatcccccg
     1681 cagttaccgc accatggaag gcttcggtat tcacaccttc cgcctgatta atgccgaagg
     1741 gaaggcaacg tttgtacgtt tccactggaa accactggca ggtaaagcct cactcgtttg
     1801 ggatgaagca caaaaactca ccggacgtga cccggacttc caccgccgcg agttgtggga
     1861 agccattgaa gcaggcgatt ttccggaata cgaactgggc ttccagttga ttcctgaaga
     1921 agatgaattc aagttcgact tcgatcttct cgatccaacc aaactgatcc cggaagaact
     1981 ggtgcccgtt cagcgtgtcg gcaaaatggt gctcaatcgc aatccggata acttctttgc
     2041 cgaaaacgaa caagcagcat tccatccagg tcatattgtt cctggtctgg acttcaccaa
     2101 cgatccgctg ttgcagggac gtttgttctc ctataccgat acacaaatca gtcgtcttgg
     2161 tgggccgaat ttccatgaga ttccgattaa ccgtccgacc tgcccttacc acaacttcca
     2221 gcgtgatggc atgcatcgta tggggatcga cactaacccg gcgaattacg aaccgaactc
     2281 gatcaacgat aactggccgc gcgaaacacc gccgggaccg aaacgcggcg gttttgaatc
     2341 atatcaggaa cgcgtggaag gaaataaagt tcgtgagcgc agtccatcgt ttggcgaata
     2401 ttattcccat ccgcgtctgt tctggctaag tcagacgcca ttcgagcagc gccatattgt
     2461 cgatggtttc agttttgagt taagcaaagt cgttcgtccg tatattcgtg agcgcgttgt
     2521 tgaccagctg gcgcatattg atctcactct ggcccaggcg gtggcgaaaa atctcggtat
     2581 cgaactgact gacgaccagc tgaatatcac cccacctccg gacgtcaacg gtctgaaaaa
     2641 ggatccatcc ttaagtttgt acgccattcc tgacggtgat gtgaaaggtc gcgtggtagc
     2701 gattttactt aatgatgaag tgagatcggc agaccttctg gccattctca aggccctgaa
     2761 ggccaaaggc gttcatgcca aactgctcta ctcccgaatg ggtgaagtga ctgcggatga
     2821 cggtacggtg ttgcctatag ccgctacctt tgccggtgcg ccttcgctga cggtcgatgc
     2881 ggtcattgtc ccttgcggca atatcgcgga tatcgctgac aacggcgatg ccaactacta
     2941 cctgatggaa gcctacaaac accttaaacc gattgcgctg gcgggagacg cgcgcaagtt
     3001 taaagcaaca atcaagatcg ctgaccaggg tgaagaaggg attgtggaag ctgacagcgc
     3061 cgacggtagt tttatggatg aactgttaac gctgatggca gcacaccgcg tgtggtcacg
     3121 cattcctaag attgacaaaa ttccggcgta aatatggata aggtcacgtc ccctggggag
     3181 tgaccttacc accttactta cacatcaaga taactaccca accgataacc acgctcagca
     3241 attgcatatt tcaacgacgc tgatgtcagc acttccagct ccgttaaccg tgggaaacag
     3301 taagcactct ggcgaatcgt attatcgaca aacgccggat ggcacatgac ttccagcgag
     3361 cgttccccgc gatggctgga atcatcaagc acttgcagga acagtgcctc gctaatctct
     3421 tcaccataaa acgcgctact gaatccgtgc gaactgcgca gattagccgg taaatcacca
     3481 gcgttagata gcggctgacg gtcgatgcgt aacgcaatcc cctcttcagc cgcaaacctg
     3541 gcaacaatag ggaaaatctg cgggaacata tgcacatgat gatggctatc aagatgcgta
     3601 ggtttgcgtc caaatagctc aatgaaacgc aaatactgac tggcaagctc ctgagtaatt
     3661 tcttccagcg gtaaagcatc ttcttctgcc aactgccaga tccattttcc cagcacacca
     3721 tcgcgggtta accccggcat agctgtcagt ggcttgccca tagtaaggac aaagtgcatc
     3781 cctatggcca gactcggttc atcacgactc aactgcaccg catggtcaat agcctgccca
     3841 ttcaccaacg ccgtcgtcga tgtgacaatc ccattgcgac aggcctcgat aatgccgtag
     3901 ttctgacctt tgcttaagcc aaaatcatcg gcattaacaa tcagtaagcg ttccataatc
     3961 agcctcggtt aatgtgcttt tttaagctct gcgatgcagt cggcaaaatt tggcagccat
     4021 ttctcgtgcg ccagaatcat ctcgcgtgcc agcagctcag catcgcgatc ggaatgcacc
     4081 aacggactaa ggtttagcgc cagtaacaca tcgttaaatt ctccgctaag tgcggcgttg
     4141 ctggcagcaa tctcgaagcc tttaatggtg tgaatcagcc ccatcacttt atcatcgaaa
     4201 tgcgtaatgc gcggatgtgg cgtcgcgcca tcgcgcccca gcttacaggt catttctacc
     4261 gcccagtctg ccggaatatt atcaatatgc ccatgatgcg ggatattaac gtaatgttca
     4321 gcttgcttgt cgttgtagat agcgttgatc acttcgcacg ctgcatcaga gtaataagcc
     4381 ccaccgcgct gttccagttc tttcggctta actttcaact ccggattttt atacagctca
     4441 aaaagttgtt tctcgacttt ctgtactacc tgtgctcgtg cgccgccttt gtagtattcg
     4501 cccatttcaa tagccagcat ctctttctgc ttgaagtaat acagcagata agaacatggc
     4561 agcagattta acgaacgaat taagccctca ctaaatggca gatcgaaaat attttttaca
     4621 gaggacgctt ttaactgccc tgacgccaca ccatcaagca attcggcaaa gcgcgacttg
     4681 ccatttacca gcacatcctt aatgaacacc atatggttga ggccgaacag atcgatagat
     4741 aaatcatcgc tgtctttcag catcagaaca tcgcgaataa acatcttcat gccgatcgga
     4801 atattacaca cgccgataaa gcgtttaaat ccggtatgac gataaacggc ttcagtgacc
     4861 attcccgccg ggttagtgaa gttaatcacc catgcattcg gacaaagttc ttcgacatct
     4921 tttacgatgt caaaaatcac cggaatggta cgcagacctt taaacagacc gcccgcgccg
     4981 ttggtttcct gaccaagata accatgactt aatggaatac gttcatccag ttcgcgcgcc
     5041 ggtaattggc ctacgcgcag ttgggtagta acgaaatcag catctttcaa tgcttcgcgg
     5101 cgatccagcg ttttataaag cttcatcggg acgccagcgt tatcaatcat ccgttggcag
     5161 agttcaaaaa tgatatccag tttctcttta ccatcttcga catccaccag ccataattcg
     5221 ctgaccggca attcgtgata acgcttaata aatccttcca gtaactccgg ggtatagctg
     5281 ctcccgccac caatagtgac gacttttaat ttctggctca taatttctcc cttcagtaca
     5341 gaatactgat atctggcata tctgcccccc cggacataaa taacccagca acaggacaga
     5401 tatgtgaatt gtcaggtata acgacttact gattaaattc agtcaatttc ttacgataac
     5461 tcttaggcgt aaaggacgtt aatttcttaa acgttttaat aaacaaactc gggctactat
     5521 aaccggcctc aaacgcaata tccgtcacgg aatagttggt catttccagt tgttttttgg
     5581 caaaattaat acggatctca ttaataatct gcattggtgt tttgccataa tatcgttgag
     5641 tcgctcgcgt caaatattcc tgtgatttgg ctgacaacgt caccatattc tccagcgccg
     5701 attcactaaa ctgctcttta tcatgcatct tttctaccgt acttttcagc cactgcggta
     5761 catcatcaat cacctgttct tcgcggtaat gacgtaaacg gttaatgaca taaaaagtaa
     5821 ccatctcaac aaactcttcc agccctgttt cgcggaaatt caatgaagaa atcactgttt
     5881 ccacataggt gagaaaagcg ttattggtcc ggtatacctg cgaagcgaca aagcaataag
     5941 gcaacaacgg caggtaatgc tgctcaaaaa agcgtttact gatcccaacg ttcaatatac
     6001 gcgtggcacc aaactcataa aaactttgat ggtgcgaacc taacggaata aaaacaaaat
     6061 cgccccgttc cagtaacacg cgcttaccgt taatctcctg gaaataacgc ccggttaata
     6121 ccagagtaaa ttcataatag tcgtgctgat gcagcccgct gatactctca gttttgttat
     6181 agataaacac atggaagttt ttgccattaa acaactgctg ttctcgggca gtggcaattt
     6241 ccggcgcgtt aatcactggc tgcatcatcg actccttatg ccttcagttt ttcatgaagc
     6301 tcaattaatt cagtaatcag ttcacgcgca agcatggacg tcattaaatg atcctgagcg
     6361 tggaccagca ccagactcac tttaatctta ccttcgcccg catcgccttc aatcagtttc
     6421 gtctgtacca gatgcgcttc attcaatgcc atccgtgact gatccatcat ggcttttgct
     6481 gcagcaaaat cgccctgctt cgcctgtttc agtgccgcat acgccaggct gcgcgcttgt
     6541 ccggagttga tgataagccc catcaccact tcttccagct cttcagcttc tgtttgcgta
     6601 tcaggaatat tatcgagatc catcatacat cgttcctctc tcttcttacc ggcacgatta
     6661 cccgtaccgg catcgattaa aatttcaggg cgttagcgat atcttcttcg ctttcttctt
     6721 tatcaatcgc attttgtgct ttgttagcca ccacaacaaa gggcagataa attaacgttg
     6781 cgatgccaag gttgaagagt gcgaccagca atgcggcgac gctaccgttg gtgttaaaga
     6841 aggctcccag accggttggc atggtccacg gtgcaatatt ggtcaccgga ggaataatgc
     6901 ccatgtagta cgctgcgagg gtgattgccg ccagaatcgg ttgtaccagt acaaacggga
     6961 taaacatcac cgggttcatg ataattggca gaccaaacag aatcggttcg ttaatctgga
     7021 agatgccgga cggcagcgcc agttttgcca cctgacgata atcagcacgg cgagatgcga
     7081 taaagatagc caggatcagg cctaaagtcg caccactgcc accgaggaaa ataaaggagt
     7141 ccagcatcgg cttggcccag atatggaagg tcttaccggc tgccagcgcc gcttcgacgg
     7201 aaccatattg ctgataggtc gcgatatttt ccagcgccca cggcgtcata atgccgttgt
     7261 ccagtgcggt cagcgccagc gcgccatgaa taccgaagaa ccagagcagt ggaacaaaga
     7321 tcacataggc ccagcccacc acgctaccca acgatgccag tggggttgag atggtatcca
     7381 taatgatctg atggaagttg gtgccccagg tattcaacgc ccaggcaata atccccatca
     7441 cggaaagaat aataaagccg ggaattaatg ccgagaagga acgcgatact gaagcaggta
     7501 cgctgtcggg cagtttaatg acccaattgc ggcggacaat aaaggtaaac atttctgcca
     7561 ccaccaggcc aataataatc ccggagatga tattcgcccc acctaaccag tttgcgccaa
     7621 ccgcataggc ctcaccgaca ctatatgggg tgacggtcat aaatgctgca acggataaca
     7681 acccggccgc cagcgcatcg actttacgct cttctgccag cgccatgcca ataaagaacg
     7741 gtgccattaa agacattatc cctaatgttc cgttatatac gttgccgcca atacctttca
     7801 gaccattaag tgtttcaatg gttgaggcgt cgaggcgaat acctaaggaa taaaaaaacg
     7861 acccctcccc aaagcttaga aaaacgttgt taattaatac aaacatggcc cccgcaaggg
     7921 ttaacggcat taagcgaata aagccatttt tgattgcatt aacgtgtggc tgctttccta
     7981 ttttaactgc aaaagggagg agtacctttt caagcgatgc aataacatta ctcatagaaa
     8041 aataccctta aaaaccgcaa tttaaatatt gcggtattga tttatgaaat aactctttga
     8101 cgggaaaatt taaaataaat taatttgctg cggctttttt aatcgctgca accgcagcct
     8161 taagcacgcc taaaccatcg actttgccat aaagcagcga gtcaattact tcaaccggtt
     8221 tgttgggtaa caaacgctgg atttcgggca acatataagc aatctgcggc cctaataaca
     8281 cgacatcggc attctgacct ttttcaccag ccagtgtttc cggaaatgct tcaataatga
     8341 ccggaacttc atatttttct gcctgtgcgc gcatttttga taccagtaaa gaggtagaca
     8401 tgcccgcaga acaaaacaga taaatgtgtt tcttttccat aaaactgccc tcatcgacga
     8461 ttatctgtca gccagacact ccgcaagcct taacctgctt ccatgctctg ggtaacttgc
     8521 gaaaccaaca tgactttttt tgtggctgaa aggagtatac ggtatagacc tgagcaatag
     8581 tatttctttg accttgtaaa ttgtctctcc ttgacctgcg gttatgaccc tcttcacatt
     8641 tcgggcaaat aattcgcgaa gataattaag aactcaggcc aaaaaaaacc ggcgcaatgg
     8701 ccggtttccg ttgttactca agctttcaga cgaagtgatt acttcgcagc ctgtggatca
     8761 gtgtcgtatt cagcacaggt ctgataaccg gagttgatga catgtccggt atcatctaac
     8821 gcgacaaagt aggtttctgc tttaccatca cgttgaccca gaatgtaggt ctggcaagta
     8881 ccacgagcat ggatcatgct cacttcagac gaaggtttac ccgcaatttg tgcaacctgc
     8941 gcccggctca tgcctttttt gacgtctttc accacaggct gtacaaactg gtctttggta
     9001 cgatcataag ccgtacaacc cgccagcatg gttaataccg ccgctgcact cagaattcct
     9061 gccatattct tgttcatatt ccgtcctctt gtttatcagc gtgttagata agcctggaat
     9121 acattgggcg ctttttcaag cccgtgaacg aaacggctcc gctttcagag gattcctgta
     9181 tgacgtttta accaccattc agcccgctgt cgcttgtcgt ttcagtagca acgggttagc
     9241 tttaaggaag ttttgtcttt tctgtctgga ggggttcaat gacattgcaa caacaaataa
     9301 taaaggcgct gggcgcaaaa ccgcagatta atgccgaaga ggaaattcgt cgtagtgtcg
     9361 attttctgaa aagctacctg cgaacttatc cgttcattaa atcactggtg ctcgggatca
     9421 gcggcggtca ggactccacg cttgccggta agctgtgcca gatggcgatt aatgagctgc
     9481 gccaggaaac cggcaacgaa tcactgcaat ttattgccgt acgcctgccc tatggtgttc
     9541 aggccgacga acaagattgc caggatgcca ttgcctttat tcaaccggat cgcgtattaa
     9601 ccgttaatat caagggcgcg gtattggcta gcgagcaggc attgcgggaa gcaggcattg
     9661 aactgagcga ttttgtccgt ggcaatgaaa aagcgcgtga gcggatgaaa gcacaatata
     9721 gcattgcggg tatgaccagc ggtgtcgtgg tgggcaccga tcatgcagca gaagccatta
     9781 ccggattctt cactaaatat ggtgacggcg gtacggatat taatccgctg tatcgtctca
     9841 acaaacgcca gggtaaacag ttgctgacgg cattaggttg cccggaacac ctttataaga
     9901 aagccccaac ggccgatctg gaagatgatc gcccttctct gccagatgaa gtggcactcg
     9961 gcgtgaccta tgacaatatc gacgactatc tggaagggaa aaacgtacct gaacaggtcg
    10021 ccagaacaat agagaactgg tatctgaaaa ccgaacataa acgccgtccg ccaattaccg
    10081 ttttcgatga tttctggaaa aagtaataat ttgcacataa tggtttgtgc gaaaaagagt
    10141 aatttgttca cgccggatgc ggcgtgaacg ccttattcga cctacaaaac tatgcaaatt
    10201 caatatattg caggagtcga ataggcctga caggcgtagc acgtcagacg gtgtaacctt
    10261 tgtcatcgac ccgcctcttt tttaatcgct tcccgcctgt tacactggat agataaccag
    10321 cattcggagt caacagtggt acggcgttta acttctccgc ggctcgaatt tgaagctgcg
    10381 gcaatttatg aatatcccga acatttacgt tcattcctta atgacttacc cacccgacca
    10441 ggggtgtatc tgtttcatgg cgaaagtgac accatgccgc tctatatcgg caaaagcatt
    10501 aacatccgca gccgcgtcct ttctcattta cgtaccccgg atgaagccgc catgctacgg
    10561 caatcccgac gcatcagctg gatctgcacc gcaggcgaaa tcggcgctct gctccttgaa
    10621 gcgcgattaa tcaaagaaca acagccgctg tttaataaac ggttgcgccg caatcgccag
    10681 ctctgtgccc tgcaattaaa tgaaaagcgc gtcgatgtgg tgtataccaa agaggtggat
    10741 ttttcacgag cccccaacct gtttggcctg tttgccaata ggcgcgcagc tttgcaagca
    10801 ttgcagagca tcgctgatga acaaaaactt tgttatggcc tgttgggact ggaaccgtta
    10861 agtcgcggtc gtgcatgttt tcgttcagcg ctaaaacgtt gcgccggagc atgctgcggt
    10921 aaagagagcc atgaggaaca tgcgctacgc ttgcgccagt ctctggagcg tttgcgggtg
    10981 gtgtgttggc cttggcaagg tgcggtggca ctgaaagaac agcacccgga aatgactcaa
    11041 tatcatatta ttcaaaactg gctgtggctg ggggcggtta attcgctgga agaagcgaca
    11101 acgttaattc ggacacccgc cgggtttgat cacgacggtt ataaaattct ttgtaagccg
    11161 ctgctttccg gtaactatga aattactgaa cttgatccgg cgaatgacca gcgagccagt
    11221 tgatttcact gaacaacagt ttaccttgcg gttgtaaaag ccgcagggtg tgcctgccat
    11281 cgaaccagca cgcgccctgg tcggtagtga gcaatttatc gcccagttgc cacgccccgt
    11341 taatcacaaa caccaccccg ccgcgtgagc caaaggtagt aaacgtacgt tcggcaattc
    11401 tgactttcgc cttacagaca tccaggcgtg tcatgatgtt gaaatccatc gacatctgcc
    11461 ctgccgtcag tttcgctttt accacctggt ccgctgcaaa ggcaaaaggc tgtaacggtt
    11521 ttaaggtatg gttaaagcgg tctgcgcttt caaggagcat ctcgccgcct tccagcaacg
    11581 tcactatcct ttccatgccg ggaaacaaag aaaactcccc attcgccgcg atggatgcaa
    11641 tgctggcacg ccagtaaaaa tcacgttttg ccggtgggaa agtgcaaatt tcgcgcgttt
    11701 caccggcagc atttcgccac agattcaccg acattttacg catatcaaag tattccatac
    11761 tcgctcccgg cccgttcttt tcattgttat cgttcaggta accgtggatg gttatcggca
    11821 actcgggtga tttacgttag tggtgatcag gcttttttta caactgtttg agaagagaaa
    11881 agaaaaccgc cgatcctgtc caccgcatta ctgcaaggta gtggacaaga ccggcggtct
    11941 taagtttttt ggctgaaaga ttattcagca gttgcaggca ttttaccttt tgccgctgga
    12001 cgttctgtca gacgcttctc aaaattagca ttaaattgct ttttctgttc cggcgtcagg
    12061 atgttgtaaa ttttgttctg ggtttccatg tgcgccagca tgttagcttt gcgctgttct
    12121 tccatttttg cgatctgcgc ttcagctttt gctttatcga aggtatcgct ggcaatgatg
    12181 tcatgcattg cgcggcgttc ttccagcggc ggacgtttca tctggtcacg ctggcctttc
    12241 atgatttcgc ggatctgctg tttctgcgcg tcggtcaggt tcaggtcttt gaacatcatg
    12301 tcctgatgcg gaccgaactt gcctttgtgg tgcatcatcg gcttcgcgtc agccggtgct
    12361 gcggtagtgg tgtctgcggc atgggccagg ttagccgcgc caagagccag ggtagaggca
    12421 acaaacagtg cagttaattt acgcatattc tatatccttc ctttcagtta tttattacgg
    12481 ctttcttaag tagcgtgccg tgttgacgag attaacttta ctgactttag cgtcaattaa
    12541 tcagagcaac ggtaaaacaa tgaaagtgta aaaaacactt ttgcgccaat tatggagaaa
    12601 aaaagaaaat ttgatggaga gtgatgagag aatattacaa cacgatgatt ttgcagagat
    12661 tatgaagaac tataccggat gactggtgat aaataaagca aataaccagg attaatctgt
    12721 attaatttat aagaaagcaa cttaataccc gcagaatgat ttctgcgggt aagtattagc
    12781 ttattttttc gagcattaat cccgcgcgta atcccaacgc taccaacgga ttagggaata
    12841 acacatactc tacatcatgg gttacggtaa aacgttcctc tccgtcctgc gccagcaatg
    12901 ttcctttctc aaacggcata aaattcagcg tgtcacttgc catatgcatt tcgaaggacg
    12961 gcgagtggcg agtaatttgc gaaaccaccc gataacggag cggcggtgtt ctcacgatac
    13021 cgacactctc accagatagc agcgcagcaa ttgcgctggc agttactgca aactggcgaa
    13081 gatcgttttg cccaaagggc aacgctttgc caagttccag cgtacaggcc agcgcgccaa
    13141 aatgtctggc gctgaaatgg gtaaacgtac caccaggttc ctgatggaac accagcgcct
    13201 ccagccccgc cgcacccagc cacgtcagaa atttctcgtc ccagggaatg tcgcgttgcg
    13261 gtaatacacc gaactgcgga tgcaaggagc cacgaattgc ggtatgtaga tcaaggtgcc
    13321 agcgcacaga ttctttgccc aggtcataaa aatcttccag gcactgttcc agttcgcgcg
    13381 cccgacaggt ttctccgctt tcagcaaata gctgccaacg accgccaaac attcgattca
    13441 tatcgctatg gcaataacgt ttcccttgct tcagcgcagg aggattcccg aggatcacca
    13501 gcaaccgcca acgtaacggg atctcgccgt gagatatcgc gccaagcaac gcgtccagca
    13561 tctccacagg tgccgtctca ttaccgtgta ttcccgctga aatcaccagt gcgccttgcg
    13621 gtggcgttaa tggcgtcagt tccagcacac catcgcccag ccagcgccag cgaacgccgt
    13681 tgatttctcg ctcggtgata accggttttt tacccgttaa ggtcagagca agaaaattat
    13741 ccattgcccc ctccctcgcg ctggaacgga taaaccgaac cgagattcag taattggctc
    13801 aatacatcca gtgcttcccg cccttcgcgc agcaattgcg ggtcggccag atcggcagca
    13861 gtaaggcgat cgcggtagta acgatccacc cagtcattga gcgcattaaa cagcgtatcg
    13921 ttcatcatca ccgccggatt caccgcccgg cgttcttctt ctgtcaatac cacccgcaac
    13981 cgcaggcacg ccgggccgcc gccattcgcc atgctttcac gtaaatcaaa gacttttagt
    14041 tcgctaatcg ggttgtcagc ggcaaggagt tcattgagat aaccccatac tccggcgtgt
    14101 tcccgacact cctgaggcag caccaacatc atggaaccat catcgcggct caacagttgg
    14161 ctgttaaaca gataggtaga caccgcatcc gacacggaaa cctgagttgc cggaacttct
    14221 atcgccataa aaccgttcac ccgcgcacgc aggtttgcca gtaactgtga ctggcgagcg
    14281 aacgcctgtt ggtggcaaaa cagcacctgg cggttactca cggcaatcac gtcattatga
    14341 aaaacgccct ggtcgataac gtccgggttt tgctgggcga aaatcacctg ttggggattc
    14401 acctgattca gccttgccac cgcctcgctg gcttcgcgag tctgtcgcgc cggataacgg
    14461 gaaggccggg tatcattgcc ttcttctcgc ccgtagacaa aaagttgcat acccggctca
    14521 ccgtaatgac cgccgagacg attgtggttt gccgccccct catcaccgag caacgctacc
    14581 tgcggcaacg ccgaatgaac gctaaatttc tcttcgtcgt taaaaatcgc ttttaaaagt
    14641 gattcggtga cgtgcgcttc cagcgagcgg tgaaatttat tgttcaggtt ggcaacggtg
    14701 agatgcactt tgccatccag cgtatcggca gatggcgcga tcgttgccgc attggctacc
    14761 cacattggcg aagcggaact gacgctggaa agccagtgcg gtgcctggcg tgcaactttt
    14821 tccagtacct gctcatcgct accactgaat cccaactgac gcagcaccgg aataaacgga
    14881 cgctcgtgcg gcgggatcac ggcctggggg aatcccgcat cggcaagggc tttcattttc
    14941 agtaagccct gttttgccgc ctgtcgcggg ttagacacct gaaaacggtg acgggtagag
    15001 gcttcattac caaacgacag gccagcgtaa tgatgcgtca gccctaccag cccgtcgaaa
    15061 ttaacttccc aggcgttcat cgcaccacct catcggaaaa atccagcccg gggttaagcg
    15121 ttgcgggcaa agttaacgag tccgactcca ggctcgccat aggccatgcg cagtaatctg
    15181 cggcatacca ggcgctgggg cgatggttac cggatgcacc aatgccgccg aatggcgcgg
    15241 tactggcagc accagtaagc ggtttgttcc agttaacaat ccccgcccgc gcctccagca
    15301 acagttgatc gaatttttcc cgctcggggg aaaccagacc gcaagagagt ccgaagcgag
    15361 tgttattcgc cattagaatc gcttcctcga aagaatcata acgccagacg cgcaataacg
    15421 gtccgaacac ctcttcatct ggtacgccag caacgcctgt catttcaatg atccccggcg
    15481 tcagcaacga tgtctctgat tgtaataagc gcggcgcaag cagggttcgt ccgcccatcg
    15541 cttccagttg ctgccaggca gtaaccacct gctgtgcggc ctgttcagaa atcagcccgc
    15601 caataaacgg ctgcggttcg tcatcccagt tgcccggcgt taatcgctgg ctgacggcaa
    15661 ccagacgagc aagaaacgca tcgccctgcg ctccgctttt gagcaataaa cggcgggcgc
    15721 aggtgcagcg ttgcccggct gtgacaaacg ccgactgaat ggtcagatgg acagccgcgt
    15781 cgatatccgc cacctcatcg ataattagcg gattattacc gcccatctca agggcgagaa
    15841 ttttctccgg ctgaccggag agctggcgat gcagctggta gccggtattg gcgctaccgg
    15901 taaacagcaa accgtcgaga tcctccagcg cactcagcgc ctgacccgtt tcacgcccgc
    15961 cctgcaccag gttcagcacg cccggcggca agccagcctg ctgccataaa cgcattaccg
    16021 cttcgccact ccacggtgtc agttcgctgg gtttaaagat aatggtgtta cctgccagca
    16081 atgccggaac gatatgtccg ttcggcaaat gaccagggaa attatacggc ccaaacaccg
    16141 ccagcacgcc gtgcgggcga tgtcgcaggc tcgccgcgcc gtctggcatt tcgctacgct
    16201 gctcgccggt acgagcgtga tacgccttaa ttgatatcgc gattttattg atcatcgccg
    16261 tcacttcggt tgccgcttcc cagcgcggct tgccggtttc tcgggcaatg attgcggtta
    16321 attcgccttt attcctttcc agcaatccgg caaagcgttc gacaacgacc tgacgttcag
    16381 ccaatgagag ccgcgcccag cgcggaaacg ccgcacgggc tgcccgacaa gcctgctcga
    16441 cctgagcggc atcggcatca ttgccttgcc ataacacctc gcccgatacc ggattacgct
    16501 tcacacgcga tgcgccctgg cccgttatcc agtcaccgtt aatccataaa gtcatgctgt
    16561 tttctcctct gcgcacaggc gcaccagacg aacgcgatcc cctgcgtggc atttgagggc
    16621 atccagttgt gcggcggtta aaatcaaacg ctcggttgcc ggatcggtac gcgccagcac
    16681 cacgcggaaa tggtgataat tttcattggc gaccaggcag gccggaaaat cgccctgcgc
    16741 aggctgccct tctgccactt ccaccagccg acttttacga atggcgcgca cgcggtcgat
    16801 gtcacactca agcgttggcc cgccgtcgaa gatgtcgata tagttgcggt aacgaaaacc
    16861 ttctttctcc agcaccgcgc gggcaggcgc ggtttgcgga tgtacctgac cgatgacgtc
    16921 ctgggcttcc tgggataaaa agtgggtata gatcggatgt ttcggcatca gttctgcaat
    16981 aaatgccttt tgcccggtgc cgcagagaaa atcggcgcgg ctaaaatcca tcgaaaagaa
    17041 gcgtttaccg aggctttgcc agaacggtga atagccgtgt tcgtcaatca ccccgcgcat
    17101 ttcagcaacc actttgtcat taaacttgtc gcgaaaagcc gccataaaca taaagcgcga
    17161 tttcgacagc aaatagccgt tgccctcttt gcgccagtcc gggtcgagaa acagcgtgca
    17221 cagctcgctg ctgccggtgt gatcgttact gagaaacagc gtcggcaatg cgttatagac
    17281 attcagctct tttgaggcgt gaaccaacgt gccgacgcga tagttgtacc agggatcgtt
    17341 cagcccaacc gccacctcaa tggcacaaat ccccgccacg gtgcctgtct cgctatcttc
    17401 cagcacgaac acatagccct gctcactttt gcgcagttcg ccttgccagg ttttgattgc
    17461 cctttcgata cgcgccgaaa gcgtggcttc attggcggga agcgacgtca ggccgccgcc
    17521 cgttttgctg gcaagctgca tcagcgccga gacatctgat cgctcaacgg gacggatgac
    17581 catcatgatg aacctcggct aacaaagtgt tcgcaagcag ctgcaaagcg atccagtccg
    17641 gtcgtcacct cttcttcgct gacattgagc gcaggcgcaa aacgcaccac gttgccaccc
    17701 gcaatcagta ccatcacgcc tgctttcgcc gcttcctgag agctctgttt cgcttgcccg
    17761 gcgtaatcgg cattcagtac acagccaatc agcaaaccta agccgcgaac ttcactgaac
    17821 aaaccatagc ggtgattaat agtattaaga cgctcaacaa accagtcgtg acgctgttta
    17881 acgccattaa gcatctctgg tgtgttgatg agctccagca ctttgcctgc caccgccgag
    17941 gccagcggat taccgccata ggtggtgcca tgagtgccaa cggtcatcac gcgggcgcac
    18001 tcttcggttg ccaacaacgc accgaccggg aaaccgccgc ccagcgcttt ggcggtagtt
    18061 aacagatcag gcgtcacgcc gtagtgcata taggcataca gttccccggt gcgcccgacg
    18121 ccggtttgta cttcatcaaa aatcaacagc gcattgtggc ggttacacaa ttcacgcaga
    18181 ccttgtaaaa acgcgttgct ggctggcacc acaccgcctt ccccctggat gggttcgaca
    18241 atcaccgcac aggtagagtc gtcaatcagc gcgctggcag agttaatatc gttatatgca
    18301 gcatgacgaa tatccgccgg cagtggcgca aaatcctgtg aataggctgg ctgcccaccc
    18361 gcactgacag taaacagcgt gcgaccatga aacgcatttt tgaacgccac gatgccgctc
    18421 ttatggctgc cgtagcggtc gtgagcgaat ttacgcgcca gttttagcgc cgcttcgttg
    18481 gcttccgcac cggagttaca aaagaagacg cgatcggcaa acgtggcgtc gatcaatttt
    18541 ttcgccagtc gcagtaccgg ctcgttggtg taaccgttgc tggtatgcca gaacttactc
    18601 gcctgttcgt tcagcgcttc acgcagttcc ggatgcgcat ggcccagcgc gttcaccgca
    18661 atgccacccg cgaagtcgat atactctttc ccctgctgat cccacaagcg cgaaccttcg
    18721 ccacgtaccg gtataaaggg tgccggagcg taaacaggta tcatccattc atcaaagttt
    18781 tcacgcgtaa ttggctgaga catagcgacc tctacagtaa ataattcgtt atttatatgt
    18841 taacaataag taatgtttgc gctgtaaatg tagattgcag ggttcgtgcc agccagtgat
    18901 aaaagtgcat aaacggcgga ggctaactgg aaatcaagga gttataacca aaccatatgc
    18961 atttaaagtg catataaagt gaatacgttt gcgatgtggg tgaataaaaa gaataaaaaa
    19021 cgcaatgtta tgcagaagta aaatataatt ctggaattgt gatcattgac gaaatttact
    19081 ggaaattact gcgccattct gacgcagcgc gcaccaaaag cgggcatttt ttgcgccatc
    19141 gttgacatca ttaacaacca tcgatcaaat cacttaacaa caggcggtaa gcaacgcgaa
    19201 attctgctac catccacgca ctctttatct gaataaatgg cagcgactat gaaatttgtc
    19261 tcttttaata tcaacggcct gcgcgccaga cctcaccagc ttgaagccat cgtcgaaaag
    19321 caccaaccgg atgtgattgg cctgcaggag acaaaagttc atgacgatat gtttccgctc
    19381 gaagaggtgg cgaagctcgg ctacaacgtg ttttatcacg ggcagaaagg ccattatggc
    19441 gtggcgctgc tgaccaaaga gacgccgatt gccgtgcgtc gcggctttcc cggtgacgac
    19501 gaagaggcgc agcggcggat tattatggcg gaaatcccct cactgctggg taatgtcacc
    19561 gtgatcaacg gttacttccc gcagggtgaa agccgcgacc atccgataaa attcccggca
    19621 aaagcgcagt tttatcagaa tctgcaaaac tacctggaaa ccgaactcaa acgtgataat
    19681 ccggtactga ttatgggcga tatgaatatc agccctacag atctggatat cggcattggc
    19741 gaagaaaacc gtaagcgctg gctgcgtacc ggtaaatgct ctttcctgcc ggaagagcgc
    19801 gaatggatgg acaggctgat gagctggggg ttggtcgata ctttccgcca tgctaatccg
    19861 caaacagcag atcgtttctc atggtttgat taccgctcaa aaggttttga cgataaccgt
    19921 ggtctgcgca tcgacctgct gctcgccagc caaccgctgg cagaatgttg cgtagaaacc
    19981 ggcatcgact atgaaattcg cagcatggaa aaaccgtccg atcacgcccc cgtctgggcg
    20041 accttccgcc gctaatttag cagctctcct ggctcaaact gggtcaggag aattaacctt
    20101 gagaaaaatc aacaaactgt cagtaatgat ttgttgcctg ccgtcctttg ttataccgtc
    20161 actgcgtttt tagttgtctg accacttctc tattatcaag tttgatatag gaaactccac
    20221 gatgaacgct gagcgtaaat ttcttttggc ctgtcttatt tttgcgctgg tcatttacgc
    20281 tatccacact ttcggtttat tcgatctgct caccgattta ccccacttac agacactcat
    20341 ccgccagagc ggattgttcg gttatagcct ctatattctg ttattcatta ttgccactct
    20401 ctttctgtta ccaggaagca tactggtgat cgctggagga atagtttttg gcccgctctt
    20461 agggacacta ctctcattaa ttgccgccac gctcgcctcc tcgtgctcat tcctgctggc
    20521 gcgctggctg ggacgtgatt tactgctgaa atacgttggt catagccata cctttcaggc
    20581 cattgaaaaa ggcattgcgc gtaacggtat tgattttctt attctgaccc gcttaatccc
    20641 gttgtttcct tacaatattc aaaattacgc ttacggatta accacaatcg ccttctggcc
    20701 ttataccctt atttcggcac tcacgaccct gcccggtatt gttatttata ccgtgatggc
    20761 aagcgatctc gccaatgaag gcattacgct gcgctttatt ttacaactct gtctggcggg
    20821 cctggcgctg tttattctcg tccagctcgc aaaactctac gcccgacaca aacatgtgga
    20881 tctgtctgct tcgcgccgca gcccacttac tcacccaaaa aatgaaggat agaacgatgt
    20941 cgcaacatta ttcagtgtca tggaaaaaag gactggctgc actctgctta ctggctgttg
    21001 ccgggcttag cggctgcgat caaaaagaga atgcggcggc aaaagtggaa tacgacggac
    21061 tttcgaacag ccaaccgctg cgcgtcgatg ccaataacca tacggtaacc atgctggtgc
    21121 aaattaatgg tcgtttcctc accgacgaca ctcgtcacgg cattgtgttt aaagatggct
    21181 ccaacggaca taaatcgctg tttatggctt atgcgacccc gaaagcattt tatgaagccc
    21241 tgaaagaggc aggtggtacg ccgggcgaaa acatgacgat ggataataaa gaaacgactc
    21301 atgtcacagg cagcaaactg gatatttcgg tcaactggca aggggcggca aaagcgtatt
    21361 ccttcgatga agtgattgtt gacagtaatg gcaagaaact ggacatgcgc tttggcggta
    21421 atttaacggc agcagaagag aagaaaacgg gttgcctggt gtgtctggat agctgcccgg
    21481 tcggcatcgt cagcaatgca acatacactt atggtgcggt ggaaaaacgc ggcgaagtga
    21541 aattcaaagg caatgcctca gttctcccgg cggataacac gctggcaacg gttaccttta
    21601 aaatcaccga ataaagccag gataaaggat gatgaagata caatcgcgta aaatctggta
    21661 ctaccgtata accctcatca tcctgttgtt cgctatgctg ctcgcatggg cgctgcttcc
    21721 cggcgtccat gagtttatca atcgcagcgt tgcggcgttt gccgccgtgg accaacaggg
    21781 tatagaacgc tttattcagt cttacggcgc actggcggca gttgtctcgt tcttgttgat
    21841 gattttacag gccattgctg caccgctacc tgcgtttttg atcacctttg ccaatgcgtc
    21901 gctgtttggc gcgttctggg gcggcttgct gtcgtggacc agttcgatgg ccggcgcggc
    21961 gctgtgcttt tttatcgcca gagtgatggg ccgcgaagtg gtggaaaaat taaccggcaa
    22021 aaccgtgctt gacagtatgg acggcttttt cactcgctac ggcaaacaca ccatcctggt
    22081 ctgtcggtta ttgccttttg tccctttcga tccaatcagc tatgctgccg gtctgacttc
    22141 aatacgtttt cgctcgtttt ttatcgccac cgggcttggt caattaccgg cgactattgt
    22201 ttattcctgg gcgggcagca tgttaacagg cggtactttc tggtttgtca ccggactgtt
    22261 tattctgttt gccctgaccg tggtgatttt tatggcgaag aaaatatggc ttgagcgcca
    22321 gaagaggaat gcctgatggg tttaccgccg cttagcaaaa ttcctttcat tttacgtccg
    22381 caggcgtggc tgcatcgtcg ccattacggc gaggtgctaa gcccgattcg ctggtggggg
    22441 cggatcccgt ttatctttta tttggtgtca atgtttgttg ggtggctgga gcgcaaacgc
    22501 tcaccgctcg atccggtagt gcgatcgctt gtcagcgcgc gcattgcgca aatgtgcctg
    22561 tgtgagtttt gcgtggatat caccagtatg aaagtcgccg agcgtaccgg cagcaccgat
    22621 aaactgctgg cagtggctga ctggcggcaa agcccgctct ttagcgatga agaacggctg
    22681 gcgctggagt acgccgaagc cgcaagcgtc acaccgccaa cagtcgatga tgccctgcgt
    22741 acccgactgg ctgcgcattt tgacgctcag gcgctcaccg aactgacggc attgatcggc
    22801 ctgcaaaacc tgtcggctcg ttttaattct gccatggaca ttcccgctca ggggctgtgc
    22861 cgtattcctg aaaaacgttc ttaaggagag atgatgcgcc attgtgggtg gttgctggga
    22921 ttgttatcgc tgttttcttt ggcaacacat gccagtgact ggcaggaaat taaaaatgag
    22981 gccaaagggc aaaccgtctg gtttaacgcc tggggcggcg ataccgcaat taaccgctat
    23041 ctcgactggg ttagcggcga gatgaaaacc cattacgcta taaacctgaa gattgttcgt
    23101 ctcgcggatg ccgcagacgc ggtgaagcgc attcagaccg aagctgctgc cggtcgtaaa
    23161 acgggcggct cggtggatct gctctgggtg aacggcgaaa acttccgcac cttaaaagag
    23221 gccaagttat tacaaacggg ctgggcggag actctgccca actggcgcta tgtcgacaca
    23281 cagctgccgg tgcgggaaga tttttcagtg cctacagaag gggctgaatc gccctggggc
    23341 ggcgcacaac tgacgtttat cgcccgccgc gatgttacgc cacagccacc acaaacgccg
    23401 caagccttac tggagtttgc taaagccaat cccggcacgg ttacctatcc gcgcccaccg
    23461 gactttaccg gcacggcgtt tcttgaacag ttgctgatta tgctgacgcc cgatcccgcc
    23521 gcgttaaaag ttgccccgga taaagccact ttcgcccgcg tcactgcgcc cttgtggcaa
    23581 tatcttgacg cgctacatcc ctatttatgg cgcaaaggaa aggatttccc gccttcgccc
    23641 gcgcggatgg atgctctgct gaaagccggc acattgcgcc tgtcgctgac ctttaaccct
    23701 gctcatgccc agcaaaaaat cgccagcggc gatttgcctg caagcagtta cagttttggt
    23761 tttaaccagg ggatgattgg caacgtgcat ttcgtcacca ttcctgctaa cgcaaatgcc
    23821 agtgctgcgg cgaaggtggt tgccaacttc ctgctctcac ccaatgcgca actgcgtaaa
    23881 gcggatcccg ctgtctgggg cgatccttct gttctcgatc cgcaaaaact gcctgatggg
    23941 cagcgcgaaa cattgcaatc aagaatgccg caagatctgc cgccggtact ggctgaaccg
    24001 cacgcaggtt gggtaaatgc gctggaacaa gaatggctac accgttacgg tacgcattaa
    24061 tctttttgct gtgggcgatg gtggcggtga tttatgcacc actgatcccg gcagctctca
    24121 cgctgatatc gcctgctttg tcgttgacac actggcaggc gttatttgcc gatccacagt
    24181 taccgcaggc attactggca acgctggtgt cgacaaccat cgcggcggtc ggggcgttgt
    24241 tgattgccct gctggtgatt gtggcgctgt ggccttggcc gaaatggcag cgtatgtgtg
    24301 cccgtctgcc gtggctgctc gccattcccc atgtggcttt tgccaccagc gcccttctgc
    24361 tctttgctga cggagggctg ctttatgact atttcccgta tttcactccg ctaatggaca
    24421 aattgggtat tgggctgggc ctcacccttg cagtgaaaga aagcgcattt ctgctgtgga
    24481 tcttagcggc agtattgagc gaaaaacggc tgttgcagca ggtcattgtg ctggattcgc
    24541 tgggctacag ccgctggcaa tgcctgaact ggctgctctt gccctccgtc gcacctgcac
    24601 tggcaatggc gatgttggcg attgttgcct ggtcgctttc ggtcgtggat gtggcgatta
    24661 ttcttgggcc aggtaatcca ccgacgctgg cagtaattag ctggcagtgg ttaactcagg
    24721 gcgacgcgga tcaacaaacg aaaggcgcac tcgccagtct gctgctgatg ctgttactcg
    24781 ccgcctacgt tttgctgagc tatctgctat ggcgcagctg gcggcgcact attccccgcg
    24841 tagatggcat tcgcaagcct gccacacctt tattaccagg cactacgctg gcgagttttt
    24901 tacccttaac cggtgtgctg tgtgtggttc tgctggcgat cctcgcggat cagtcgacga
    24961 tcaatagtga agcactcatc aacagcctga caatggggct ggtggcgaca ttcatcgctt
    25021 tgctcctgtt actgctgtgg ctggaatggg ggccacagcg tcgccagttg tggctctggt
    25081 tgcccattct attgcccgct ctgccactgg tggcgggcca gtacacgctg gcgctatggc
    25141 tgaacctgga tggaagctgg acggcggtgg tctgggggca tctgctgtgg gtaatgccgt
    25201 ggatgctgtt tatcctgcaa cccgcctggc agcgcattga ttcacggtta attttgattg
    25261 cgcaaacact gagctggtcg cgggccaaaa tcttctttta cgtgaaatgt ccgcttatgc
    25321 tacgccctac gctgattgcc ttcgcggtgg gattttcagt cagtattgcg cagtatatgc
    25381 cgacgctgtg gctgggcgcg gggcgttttc cgacgctcac cactgaggcg gtggcattaa
    25441 gcagcggcgg cagcaatggt attttcgccg cccaggcttt atggcaactg ctattaccgc
    25501 ttattatttt tgccctgacc gcgttagtcg caaaatgggt aggttatgtc agacaaggac
    25561 tccgctaatg ctctgcgtga aaaatgtttc gctacgttta ccagaaagcc gcttgctgac
    25621 aaacgttaac tttacggtgg ataaaggtga cattgtcacg ttaatggggc cgtctggctg
    25681 tggaaaatcc actctgtttt catggatgat tggtgcactg gctggacagt tttcttgtac
    25741 aggtgagcta tggctcaatg agcaacggat tgacatgcta cccaccgcac agcgtcagat
    25801 tggcattctt ttccaggatg cactgttatt tgaccagttc agcgtcgggc aaaatttact
    25861 gctggcgcta ccgtcgacgc tcaaggggac tgccaggcgc aatgccgtga aagacgcgct
    25921 tgatcgagcg ggactggctg aaacttatca ccaggaccct gcgacattat caggcggtca
    25981 gcgagcgcgg gtggccctgc tgcgcgccct tctcgctcag ccaaaagcgt tactcctgga
    26041 tgagccattc agccgtcttg atgtggctct gcgcgataat tttcgccagt gggtgttcag
    26101 cgaagttcgc gaactggcga tccccgtcgt tcaggtaacg cacgatctcc aggatgttcc
    26161 tgctgatagt tctgttctgg atatggcgca gtggtcagaa aattacaaca aactgcgata
    26221 acgcaaagtt tttctcaatg cgtcagttca gaatggcgca ctcaaaacta caatgtcggg
    26281 attttcgatg aaacgtgttt ctcaaatgac cgcgctggca atggctttag ggctggcttg
    26341 cgcttcttcg tgggccgctg aactggcgaa gcctcttaca cttgaccagc ttcaacaaca
    26401 aaatggcaaa gcgatagata ctcgccccag cgcgttttat aacggctggc cacaaacctt
    26461 aaatggccct tctggtcatg aacctgccgc cttaaacctc tctgccagct ggctcgacaa
    26521 aatgagcacc gaacagctca acgcgtggat caagcaacat aacctgaaag ccgatgcccc
    26581 ggtggcgctg tacggtaatg acaaagatgt cgacgccgtc aaaacgcgac tgcaaaaagc
    26641 aggttttacg catatctcca ttctgagtga cgcgctaagc gaaccttccc gtctacaaaa
    26701 actgccgcac tttgagcagc tggtttatcc gcaatggctg cacgacctgc aacaaggtaa
    26761 agaggttacg gcgaaacctg ccggtgactg gaaagtcatt gaagcggcct ggggcgctcc
    26821 taagctttac cttatcagcc atattcccgg tgctgactac atcgatacca acgaagtgga
    26881 aagcgaaccg ctgtggaaca aagtttctga tgaacaacta aaagcgatgc tggcaaaaca
    26941 cggcattcgc catgacacca cggtcattct gtacgggcgt gacgtatacg ctggagcgcg
    27001 tgtggcgcaa attatgctct acgcaggcgt gaaagatgtg cgtctgctgg atggcggctg
    27061 gcaaacctgg tccgacgcgg gactgcctgt tgagcgagga acgccaccga aagtgaaagc
    27121 ggaaccggat ttcggcgtga agatcccggc acaaccgcag ttgatgctcg atatggaaca
    27181 ggcccgtgga ctgctgcatc gccaggatgc atcgctggtg agcattcgtt cgtggccaga
    27241 atttatcggt acgaccagcg gttacagcta tattaaacca aaaggtgaaa tagccggagc
    27301 acgttgggga cacgctggta gcgactcgac gcatatggaa gattttcata acccggatgg
    27361 caccatgcgc agcgccgatg atattaccgc tatgtggaaa gcgtggaata tcaaaccaga
    27421 gcagcaagtt tcattctact gcggcaccgg ctggcgcgcg tccgaaacct ttatgtacgc
    27481 acgcgccatg ggttggaaga atgtttccgt gtatgacggc ggctggtacg aatggagcag
    27541 cgatccaaaa aatccggtag caaccggtga acgcggcccg gacagtagca aataacattg
    27601 caattactga cgctggtgcg acttcagcgt cagataccca ctccacaccc gcgtaaaggt
    27661 cgtcatccag cacaacgcac cgaagatcca cgcaaaccac gcaaaccatg cagggaataa
    27721 acatcccagc acaaacagta agatcgtttc tgtgccttcg gttaacccgc ccagataata
    27781 aaacgattta tgcgcataac cgggattatc aatctgatgt ttcgccgcca gcgcagcaaa
    27841 agcgagaaaa ctgctgcctg tgccgataaa cgcaaacagc agccagccgc ctgccagggc
    27901 gttttgctgc ggtgcggcga ggataaaccc aaaaggtacc agcgcgtaaa aaagaaaatc
    27961 gagagaaata tcaagaaagc cgcccgcgtc ggtaagccct ctgcgccgcg ccagcgcacc
    28021 gtccagacca tcaagcagcc tgttcaacaa aatgacgaca agcgccgcca gataccagcc
    28081 cagcgccaga aaaggcagtg ccagcacgcc gatggcaaat cccactaatg ttaaaccatc
    28141 tggcgtaatg cccggtttat caagaacccg cacgcactga tgcaacaacg gtttaatccg
    28201 gggatgaaga tggcggtcta gcacaggcac tccttaaata taaagccttt ctgattgagc
    28261 aacagtgcgg atattatggc atttttcgct tatctgcccg tgtgtaattt atgaaaatga
    28321 ttgaagttgt tgccgccatc attgaacgtg atggcaaaat tttactcgcg caacgccccg
    28381 cccagagcga tcaggcggga ttatgggagt ttgccggtgg taaagtcgag ccggatgaaa
    28441 gccagcggca ggcgctggtg cgtgagttac gcgaagaact gggcatcgaa gcaactgtgg
    28501 gtgagtatgt taccagccat cagcgagaag tttcgggccg gattatccat cttcatgcct
    28561 ggcacgtacc cgacttccac gggacgttac aggcacatga acatcaggcg ctggtctggt
    28621 gctcacctga agaggcgctg caatatccgc tggcccctgc tgacattcca ttattagagt
    28681 cgtttatggc tttacgcgcc gccagaccag cggattagtg ctaagggttt tgtcatcccg
    28741 ctggcattgc agcagtattc cctcggcttt aattaccgcg ccttcagaat aatttttatc
    28801 ctgataaacg cagcactgag tacagggctg cgctgactgc ccgcctgaac tgaacacttc
    28861 tggcggtacg tttacctcca cgtccggacg ataatgcggg ttagccagtg caattaatgg
    28921 aaatgctaat actacggcga acaatgctcg actcacaggg aactccttaa cgttattgtc
    28981 tctgctactg ataacggtag ccgggtggca aaactttagc gtctgagtta tcgcatttgg
    29041 ttatgagatt actctcgtta ttaatttgct ttcctgggtc atttttttct tgcttaccgt
    29101 cacattcttg atggtatagt cgaaaactgc aaaagcacat cacataaac
//
LOCUS       DAJOWB010000051        28689 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJOWB010000051 DAJOWB010000000
VERSION     DAJOWB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.051"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(246..464)
                     /gene="infA"
                     /locus_tag="OHO85_002420"
     CDS             complement(246..464)
                     /gene="infA"
                     /locus_tag="OHO85_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCQ2671782.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(749..1453)
                     /gene="aat"
                     /locus_tag="OHO85_002421"
     CDS             complement(749..1453)
                     /gene="aat"
                     /locus_tag="OHO85_002421"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCQ2671783.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="OHO85_002422"
     CDS             complement(1495..3216)
                     /gene="cydC"
                     /locus_tag="OHO85_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCQ2671784.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="OHO85_002423"
     CDS             complement(3217..4983)
                     /gene="cydD"
                     /locus_tag="OHO85_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCQ2671785.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="OHO85_002424"
     CDS             complement(5106..6071)
                     /gene="trxB"
                     /locus_tag="OHO85_002424"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCQ2671786.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            6615..7109
                     /gene="lrp"
                     /locus_tag="OHO85_002425"
     CDS             6615..7109
                     /gene="lrp"
                     /locus_tag="OHO85_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCQ2671787.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            7244..11350
                     /gene="ftsK"
                     /locus_tag="OHO85_002426"
     CDS             7244..11350
                     /gene="ftsK"
                     /locus_tag="OHO85_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCQ2671788.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPV
                     APQPQYQQPQQQVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            11505..12116
                     /gene="lolA"
                     /locus_tag="OHO85_002427"
     CDS             11505..12116
                     /gene="lolA"
                     /locus_tag="OHO85_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCQ2671789.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12127..13470
                     /gene="rarA"
                     /locus_tag="OHO85_002428"
                     /gene_synonym="mgsA"
     CDS             12127..13470
                     /gene="rarA"
                     /locus_tag="OHO85_002428"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCQ2671790.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            13561..14853
                     /gene="serS"
                     /locus_tag="OHO85_002429"
     CDS             13561..14853
                     /gene="serS"
                     /locus_tag="OHO85_002429"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCQ2671791.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15092..17536
                     /gene="dmsA"
                     /locus_tag="OHO85_002430"
     CDS             15092..17536
                     /gene="dmsA"
                     /locus_tag="OHO85_002430"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HCQ2671792.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPQWASQITGIPAEKIIQLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNTGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCVL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            17547..18164
                     /gene="dmsB"
                     /locus_tag="OHO85_002431"
     CDS             17547..18164
                     /gene="dmsB"
                     /locus_tag="OHO85_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCQ2671793.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            18166..19029
                     /gene="dmsC"
                     /locus_tag="OHO85_002432"
     CDS             18166..19029
                     /gene="dmsC"
                     /locus_tag="OHO85_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCQ2671794.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(19065..19691)
                     /locus_tag="OHO85_002433"
     CDS             complement(19065..19691)
                     /locus_tag="OHO85_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCQ2671795.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDTPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            20005..21153
                     /locus_tag="OHO85_002434"
     CDS             20005..21153
                     /locus_tag="OHO85_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2671796.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            21363..22793
                     /locus_tag="OHO85_002435"
     CDS             21363..22793
                     /locus_tag="OHO85_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCQ2671797.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGRGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(22794..23679)
                     /locus_tag="OHO85_002436"
                     /pseudo
     CDS             complement(22794..23679)
                     /locus_tag="OHO85_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345431.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            23787..24377
                     /locus_tag="OHO85_002437"
     CDS             23787..24377
                     /locus_tag="OHO85_002437"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HCQ2671798.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(24459..25199)
                     /gene="pflA"
                     /locus_tag="OHO85_002438"
     CDS             complement(24459..25199)
                     /gene="pflA"
                     /locus_tag="OHO85_002438"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCQ2671799.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(25391..27673)
                     /gene="pflB"
                     /locus_tag="OHO85_002439"
     CDS             complement(25391..27673)
                     /gene="pflB"
                     /locus_tag="OHO85_002439"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCQ2671800.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQTDLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(27728..28585)
                     /gene="focA"
                     /locus_tag="OHO85_002440"
     CDS             complement(27728..28585)
                     /gene="focA"
                     /locus_tag="OHO85_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCQ2671801.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         7110 a         7088 c         7489 g         7002 t
ORIGIN      
        1 ttgctaccaa acctcatggg tggcaacggg gcgctactat agggagttgg agtaaaacgg
       61 tcaagaagaa ttttaatgat aattattgtt tgctcatact gtaaacaagt tgtgcagtat
      121 atctacatcg agacaagtta cggacttata cttccaaagt acttcataca tatcacaaaa
      181 taaaaaggcc ggttaaaccg accttttact cgttctttct cttcgcccat caggcggtaa
      241 aacaatcagc gactacggaa gacaatgcgg cctttgctca ggtcgtacgg ggtcagttca
      301 acagtcactt tgtcgcccgt caggatgcgg atgtagtttt tgcgcatttt accggagatg
      361 tgtgcagtaa ccacgtgacc gttttctaac tctacgcgga acatggtatt aggcaacgtt
      421 tcaagaacgg taccttgcat ttcaatattg tcttctttgg ccatctaatc ctctggggta
      481 tcactaccgt aatttgaacc ggcaagataa tgccgaagtt ctgtaaataa gtaaagattt
      541 gcgcgctaaa tcgcaacaaa caggttcggc acattactcc gaaaacacac ggctaagccg
      601 caccaaaagc gcaacgtata agggagcggt gagataaacg atgggcgtta cctgacgcga
      661 aaaaatcctt atcggcagcg gggtaatgag cgtaaccaac tctgcgaccg caattataac
      721 actctgggga gaaatgtgcc gaaaacattc attcttgtgg tgaaaacaag catcgtggta
      781 cccagaaatt attcggcaat cgtccgaggc gcatttgatt gagataatta aggtaatccc
      841 ggcggggaat ttcgcaggca ccaagcgatg ctgtgtgatc gttaaggacc tggcagtcga
      901 taagcttacc gccatgaccg ataaattcct cacagaatac cagaagcgcc gttttagacg
      961 cattttccat ccggctgaac atggactcgc cacaaaatag cgttccctgg gccacgccgt
     1021 acataccgcc gacaagctca tcttcacgcc agacttcaat ggagtgggca tggccgagtt
     1081 cgtgaaggcg atggtaggct tcgaccacgc cacgcgtgat ccaggttcct tcttcgcgat
     1141 cgctggcaca gccttcaatg acctgaccaa aagcgtaatt catcgtgaca cgatagggcg
     1201 agcgtttatg aaatcgcttc atactacggc tgatatgcag tgattctggc catagcaccg
     1261 cgcggggatc gggcgaccac cagaggatgg ggtcgcctgg agaaaaccac ggaaaaatac
     1321 cacgctggta agccattaac aggcgcgcag ggctaagatc gcccccaagt gccagcaggc
     1381 cgttaggctc acgtaatgcg ccttccgggg aagggaaggc tattgaatgg cgagaaagct
     1441 gaaccaggcg catgaccgca aaactccacg caagtcggat cgttcaataa tagcttacaa
     1501 accctgcttg aactggtaat aacgcccctg tctggcaagc agctctgcgt gagtaccttg
     1561 ctcaataatt tgcccgttgt ccatcactat tatttgttgg aaacgagaga gtccgcgaag
     1621 tcgatgggtg accattaaca ccgttttctc acgcatcatt tctgcaagca attcaaggat
     1681 ctggctttcg gttgtggcat ctaagccttc ggtaggttca tccagcaaca ccagtggcgc
     1741 atcatgtaac agcgcacggg cgatagccag acggcgcagt tcaccaccgg agagctggcg
     1801 tccgccttca cctaaccaac tgttgagacc tgcatcctcg agcagcttct ccagaccaac
     1861 gcgacgcaag ctctccgcca gagcctcatc actactgcca ggcgaggcga gtaacagatt
     1921 atcacgcagc gtggcgctaa acagatgcac tcgctgagga acaacgctga tggtctgtcg
     1981 tagagccgct tcattcaggc tggctatggg gctatcgtta agcaaaatct ctccctgttg
     2041 cgggtcccaa gcgcgggtca gcagttgtaa cagtgttgat ttgccgcatc cggttcgccc
     2101 gagaatcgca atatgttccc cggcgtttac ctgaagagaa atccctttaa gtgcctgttg
     2161 ggattgctcc ggataagtga actgaacatc ccgtaatgtc agcgaaacgc gatcggcaac
     2221 acgagtttgg gtatcaggga aggtgacttc cggtttttga tccgttaaat cagtgatccg
     2281 aacggcagag gcaatgactt gccccagatg ctgaaatgca cccgttactg gtgccagtgc
     2341 ttcaaacgcg gctaacgcgc agaagacaaa cagggcaatt aacgcgccgg gttgagcatt
     2401 gccgccaacg ccgccagacg ccatccacag catcaggatc accgctaacg cgccaatgag
     2461 cagcattatc gcttgcgaca atgcggtcag ttcagattga cggcgttgcg cttccagcca
     2521 ttgaatttct gtattctcta gttgcgtgcg ataacgatcg ctggcgccaa aaatggtcag
     2581 ctcagcttgc ccttgcagcc aggccgtcag ttgttggcga tactgtccgc gaagatgagt
     2641 cagattttgc ccggtgcttt ttcccgcacg ataaaacagc ggtggcatca ggaaaagcgt
     2701 cagtaacata atgccgccca gcgtaaaggc gagggtgaaa tcaaggaaac ttaacccgat
     2761 tgtcaccacc ataatcacca caaaagcgcc caccagcggc gagataacgc gcagataaag
     2821 atgatcgagc gtatcaacat ccgccaccac gcgattgagc aattcgccct gacgatagcg
     2881 cgccagtccg gcaggggaga ggggcagcaa tttgctgaag gtgtaaatgc gcagatgctg
     2941 caacacgcgg aaagtcgcgt cgtgacttac cagacgttca aaataacgcc cggcagtacg
     3001 ggtgattgct gcgccacgca cgcccgcagc gggtagcata tagttgaagc tgtacagtcc
     3061 ggcaaccccc gcaaccgctg aggccgagag gaaccagccg gaaagtgtca acagaccgat
     3121 actggcgagc agcgtgacaa ttgccagcac gataccaaga cttaacatcc atttatgacg
     3181 tttatacagt gccagatagg gtagcaaagc gcgcatttaa atctcctcct gacgatgggc
     3241 cagtaatgtg gcgaatgggc caccagccac acttaattcc gcgtaacgtc cttgctcaat
     3301 aatccggcca tcctgcataa cccaaatgac atcccagtca gcaagatctt ctaactggtg
     3361 ggtgaccatt aacgttgtct ggcgcagaga ggcggcattc agcgcctcca ttacgcgctg
     3421 ttcactgtga gcatcaaggc tggcagcggg ttcatccaac agtaatagcg aacagggatt
     3481 tagtaacgca cgggccaccg ccacgcgctg cgcctgcccc acggaaaggc gggcagcctg
     3541 gtcgccaaca ggtgtatcaa cgccttgtgg caggagcggt agaaactcgc tgacccaggc
     3601 gttatccagc gctgcttgta attcttgttc gctggcatca ggtcgcgcca gtagtacgtt
     3661 atcccgcaat gttgctgccg gtaattgtgg gttttgccca acccaggaga gatgtttacg
     3721 ccatgattct ggtgataaat cgcgtaattc tatcccgttg attcgtaatg atccctgata
     3781 tgagagaaaa ccagaaagcg cgttcagcag tgagctttta cctgaaccgc tgcgaccaac
     3841 caacactgca cgttggcctg ctggcaaagt aaagttcagc ggtccggcca gcgttttacc
     3901 ttccggcgac gtgataaaca gatcctcggc ctcaatggtc accggatcgg tcgatgctaa
     3961 ttccacctcg ccacgctgcg gatgggcgag cggggtttcc ataaacgttt tcagactgtc
     4021 agctgcacca acagcctggg ctttagcatg ataaaacgta ccgagatcgc gcaatggctg
     4081 gaaaaactct ggcgcaagga tcagggccag aaaacccgca gccagcgtca caccggtatc
     4141 gtagtgacca aaatccagct cgccgagata ggaaaaacca aagtagaccg ccaccagagc
     4201 aattgacagc gaggtaaaaa attcgagaat gccggaggat aaaaacgcca gccgtagcac
     4261 ttccattgtc cgttggcgga aatcttccga agcagaacga atactttcaa tttcagcttc
     4321 accacgacca aaaatacgca atgtttccat gccgcgcagg cgatcgagga aatgcccact
     4381 taagcgagca agagcgagaa agttacgccg gttagcatcg gcagccccca tcccaaccag
     4441 cgccataaac aacggaatta acggtgctgt gcccagcaga atgagcgccg cagcccagtt
     4501 agaggggaag atcgccacca caatcagcaa cggcaccgac actgccagcg ccatttgcgg
     4561 caggtagcgt gcatagtaat catgcatatc gtcaatttgc tcgagtacca acgtcgccca
     4621 gctccccgca ggtttaccct gaatccacgc tggccctgct tgttgcagac ggtcgagaac
     4681 ctgacggcgg atggcaaagc ggatatgctg cccggcgtga taacccaccc gttcgcgtaa
     4741 ccagaccacc catgcgcgca gtacaaaggt cagaaccagt aacgtaaagg gaagcagcag
     4801 ggcttcacgg ggaatattct ccataatcat atgttgcaga attcgcgcca tgaaccaggc
     4861 ctgggcaatg atcaatatgc cgcttacaaa gcccagcaga cgagaaatat tcagccaacg
     4921 ttgggagatg acgctttgct gttttaacca gcgggttaac tctttttgac gggatttatt
     4981 cattgcacgc ttagcaggtg agttatcaga attatttgca gagcaatgtt acaacgggga
     5041 aaaaataaag gcgacccata gtcgcatggt gtcgccttct ttacttttgt tactgatttg
     5101 taaaattatt ttgcgtcagc taaaccatcg aggtagcgtt ccgcatcaag tgctgccatg
     5161 cagcctgtac cggccgaagt aatggcctga cgataaatat gatccatcac gtcacccgcg
     5221 gcaaagacgc caggaatgct ggtctgggtg gcattaccat gaatacccga ctgcactttg
     5281 atgtagccgt tttccagttc cagctgccct tcgaaaatgg cagtattcgg gctgtgaccg
     5341 atagcaacaa acagaccagc aacgtcgagt gactcgatgt tatcgctgtt ttgcgtatcg
     5401 cgcagacgaa cgccagtgac acccatttga tcgccggtca cttcttccag cgtacggttg
     5461 gtgtgcagaa tgatgttgcc gttctccact ttatccatca gacgcttaat aaggattttt
     5521 tccgcgcgga aaccgtcacg gcggtgaata agatgcactt ccgaagcgat gttagacaga
     5581 tacagcgcct cttcaaccgc ggtattgccg ccgccgatga ccgcaacttt ctggttgcga
     5641 tagaagaaac cgtcgcaggt tgcacaagca gaaaccccac ggcctttaaa ggcttcttca
     5701 gagggcaggc cgagatagcg tgcagaagct ccggtggcaa taatcagcgc gtcgcaagtg
     5761 tattcgccgt tatcgccatt cagacggaac ggacggtttt gcagatccac cttgttgata
     5821 tgatcaaaaa tgatctcagt ttcaaacttg gtggcatgtt cgtgcatgcg ctccattaat
     5881 aacggaccgg tcagatcgtt tggatcgcca ggccagtttt ccacttccgt ggtggtggtc
     5941 agttggccgc ctttttccat gccggtaatc agcacaggtt gcaggttggc gcgcgccgcg
     6001 tagacagcag cggtgtatcc cgccgggcct gaacccagga taagcagttt actgtgtttg
     6061 gtcgtgccca tgagatcccc atagttgttg gcagacaatg ggcaggattg tagggaattt
     6121 acagacgtaa aaaaagagta tgacgatttt gttaacaatt tgtgcaatcg gcagcatcga
     6181 taagcaggtc aaattctccc gtcattatca cctctgctac ttaaatttcc cgctttataa
     6241 gccgattaaa tgatgaataa acgcccctgt taatgaatat ctggcatgtt gtactaaaaa
     6301 tcgatgtttt gctttgacaa tcccctggtg ttttgcgaaa acattcgagg aagaaaaaaa
     6361 cagtattctt atatgcgcat aaccatgcat gtaaatacca tgtttaccgt gctagtgaaa
     6421 tctacgtatg gcgtggacag acgccattcg tgatgtcgat agctgccaca aggcaacggt
     6481 cttctcaccg tagacccagg cattgcgcgc cgtgaatctt catgatttcg gtctatcgtg
     6541 acgggtagcg actctgaaca gtgatgtttc agggtcagac aggagtaggg aaggaataca
     6601 gagagacaat aataatggta gatagcaaga agcgccctgg caaagatctc gaccgtatcg
     6661 atcgtaacat tcttaatgag ttgcaaaagg atgggcgtat ttctaacgtc gagctttcta
     6721 aacgtgtggg actttcccca acgccgtgcc ttgagcgtgt gcgtcggctg gaaagacaag
     6781 ggtttattca gggctatacg gcgctgctga acccccatta tctggatgca tcacttctgg
     6841 tattcgttga gattactctg aatcgtggcg caccggatgt gtttgaacaa ttcaataccg
     6901 ctgtacaaaa acttgaagaa attcaggagt gtcatttagt atctggtgat ttcgactacc
     6961 tgttgaaaac acgcgtgccg gatatgtcag cttaccgtaa gttgctgggg gaaaccctgc
     7021 tgcgtctgcc tggcgtcaat gacacacgga catacgttgt tatggaagaa gtcaagcaga
     7081 gtaatcgtct ggttattaag acgcgctaac acggaacagg tgcaaaatcg gcgtattttg
     7141 attacactcc tgttaatcca tacagcaaca gtactggggt aacctggtac tgttgtccgt
     7201 tttagcatcg ggcaggaaaa gcctgtaacc tggagagcct ttcttgagcc aggaatacac
     7261 tgaagacaaa gaagtcacat tgacaaagtt aagtagcggc cgccgccttc tggaggcgtt
     7321 gctgatcctt attgtcctgt ttgccgtctg gttgatggct gccttactaa gctttaaccc
     7381 ttcggacccc agctggtcgc aaacggcctg gcatgaacct atccataatt taggtgggat
     7441 gcccggtgcg tggttggcag atacgctgtt ctttattttt ggcgtgatgg cttacaccat
     7501 tcccgtcatt attgtcggcg gttgttggtt tgcctggcgt catcagtcca gcgacgaata
     7561 cattgattat tttgccgttt cgctacgcat cattggcgtt ttggcgctca tccttacctc
     7621 ctgtggtctg gcggcaatca acgctgacga tatctggtat tttgcctccg gtggcgtcat
     7681 tggcagctta ctaagtacta cgctacaacc actgctacac agtagcgggg gaactattgc
     7741 gctgctctgc gtttgggctg cgggcctgac gctgttcacc ggttggtcat gggtgaccat
     7801 tgctgaaaaa ctcggcggct ggattttaaa cattctcacc ttcgccagta atcgtacccg
     7861 tcgcgatgat acctgggtcg atgaagatga atatgaagat gacgaagagt atgaagatga
     7921 aaaccacggc aaacagcatg aatcacgccg tgcccgtatt cttcgcggcg cgctagcgcg
     7981 tcgtaaacgg ttggcggaaa aattcattaa tccgatgggg cggcaaacag acgctgcgtt
     8041 gttctccggt aagcggatgg atgatgacga agagattacc tacactgcac gcggtgtggc
     8101 tgccgacccg gacgacgtcc tattttcggg caatcgtgca acgcagccag aatatgacga
     8161 atacgatcca ttattaaacg gtgcgccaat taccgaacct gtcgctgtag cagctgctgc
     8221 taccacggcg acacaaagct gggctgcgcc ggttgaacct gtgactcaga cgccgcctgt
     8281 tgcctctgtt gatgttccac cctcgcaacc tacagtagcc tggcagcctg taccgggtcc
     8341 acaaacggga gagccggtta ttgctcctgc accggaaggt tacccacagc agtcacaata
     8401 tgcgcagcct gcagtgcaat ataatgagcc gctgcaacaa ccagtacagc cgcagcagcc
     8461 gtattatgca cctgcagctg aacaacctgc gcaacaacct tactacgctc ctgcggcgga
     8521 acaacctgtt cagcaaccgt attacgcaac tgcgccggaa cagcctgcac aacagccgta
     8581 ttacgcccct gcgccagaac aaccggtggc aggtaacgcc tggcaagccg aagagcagca
     8641 atccactttt gctccacagt ctacatacca gactgagcaa acttatcagc aaccagccgc
     8701 tcaggagccg ttgtaccaac agccgcaatc cgttgaacag cagcctgttg tggagcctga
     8761 acccgttgta gaagagacaa aacccgcgcg tccgccgctt tactattttg aagaagtgga
     8821 agagaagcga gcccgtgaac gtgaacaact tgcggcctgg tatcaaccga ttccagaacc
     8881 ggttaaagaa ccagaaccga tcaaatcttc gttgaaagcg ccttctgttg cagcagtacc
     8941 tccagtagaa gccgctgccg ctgtttctcc gctggcatct ggcgtgaaaa aagcgacact
     9001 ggcgacgggg gctgccgcaa ccgttgccgc gccagtcttc agtctggcaa atagcggtgg
     9061 accgcgtccg caagtcaaag aggggattgg cccgcagttg ccacgaccga aacgtatccg
     9121 cgtgccaact cgtcgtgaac tggcgtctta cggtattaag ctgccctcac agcgtgcggc
     9181 ggaagagaaa gcccgtgaag cccagcgcaa tcagtatgat tctggcgatc agtataacga
     9241 tgatgaaatc gatgcgatgc agcaggatga actggcacgt cagttcgccc agacacagca
     9301 gcaacgctat ggcgaacagt atcaacatga tgtgcccgta aacgcagaag atgcagatgc
     9361 tgcggcagag gctgaactgg ctcgtcagtt tgcgcaaact caacaacaac gttattccgg
     9421 cgaacaaccg gctggggcga atccgttctc gctggatgat tttgaatttt cgccaatgaa
     9481 agcgttgctg gatgatggtc cacacgaacc gttgtttacg ccaattgttg aacctgtaca
     9541 gcagccgcaa caaccggttg caccgcagca gcagtatcag cagccgcaac aaccagttcc
     9601 gccacagcag caatatcagc agccgcaaca gccggttgcg ccgcagccgc agtatcagca
     9661 gccacaacag caggttgcgc cgcagccaca atatcagcag ccgcaacaac cggttgcgcc
     9721 acagccgcaa tatcagcagc cgcaacaacc agttgcgccg cagccgcagt atcaacagcc
     9781 gcaacaaccg gttgcaccgc agcagcagga taccctgctt catccgctgt tgatgcgtaa
     9841 tggcgacagc cgtccgttgc ataaaccgac gacgccgctg ccttctctgg atttgctgac
     9901 accgccgccg agcgaagtgg agccggtaga tacctttgcg cttgaacaaa tggctcgcct
     9961 ggtggaagcg cgtctggctg atttccgtat taaagccgat gtcgtcaatt actctccggg
    10021 gccggttatc actcgctttg aacttaacct ggctcctggc gtaaaagcgg cgcgcatttc
    10081 taacttgtca cgggaccttg cccgttcact ttcgacggtg gcggtgcgtg tcgttgaagt
    10141 tattcctggc aaaccctatg taggtctgga gttaccaaat aaaaaacgac aaaccgttta
    10201 tctgcgcgaa gttttggata acgccaaatt ccgcgataat ccgtcgccat taaccgtggt
    10261 gctgggtaaa gatatcgccg gtgagccggt ggttgccgat ctggcgaaaa tgccgcactt
    10321 gttggttgcg gggactaccg gttccggtaa atctgtcggt gtgaacgcga tgatcctgag
    10381 catgctttat aaagcacagc cagaagatgt gcgtttcatc atgatcgacc cgaaaatgct
    10441 ggagctttcg gtttatgaag gcattccgca tctgttaacg gaagtcgtta ctgatatgaa
    10501 agatgccgcc aacgcgctgc gctggtgtgt taacgagatg gagcgtcgtt ataaactgat
    10561 gtctgcgctg ggtgtgcgta atctggcggg ttataacgaa aaaattgctg aagccgatcg
    10621 catgatgcgt ccgattccag acccgtactg gaagccgggt gacagtatgg atgcccagca
    10681 tccggtgctg aaaaaagaac catacattgt ggtgttggtt gacgaatttg ccgacctgat
    10741 gatgacggta ggtaaaaaag tggaagagct gatagcacgt ctggcgcaaa aagcccgtgc
    10801 cgcgggtatc cacctcgtac tggcaactca gcgtccatcg gttgatgtta ttactggtct
    10861 gattaaagcg aatattccga cccgtatcgc ctttaccgta tccagtaaga ttgactcacg
    10921 taccattctt gatcaggctg gcgcggaatc actgctgggg atgggggata tgctctactc
    10981 tgggccgaac tccacgttgc cggtacgtgt ccatggtgct tttgttcgcg atcaggaagt
    11041 tcatgccgtg gtgcaggact ggaaagcgcg tggtcgccca cagtatgttg atggcatcac
    11101 ttccgacagt gaaagcgaag gtggcgcggg cggtttcgat ggcgctgaag aactggatcc
    11161 gttgttcgat caggcggtgc agtttgtcac tgaaaaacgc aaagcgtcaa tttctggcgt
    11221 acagcgtcag ttccgcattg gttataaccg tgcagcgcgt attatcgaac agatggaagc
    11281 gcaggggatt gtcagcgaac aggggcacaa cggtaatcgt gaagtgctgg ccccaccgcc
    11341 gtttgactaa ttaatgcatt gccggataag gcgcggtagc gtcgcatccg gcactctatc
    11401 aactgaaaat tcagtatttt cttctttcct caagctgatt attagcctgg aatagagagt
    11461 agagggaact cccgatcggg agtgacgtaa tttgaggaat aatgatgaaa aaaattgcca
    11521 tcacctgtgc attactctca agcttagtag caagcagcgt ttgggctgat gccgcaagcg
    11581 atctgaaaag ccgcctggat aaagtcagca gcttccacgc cagcttcaca caaaaagtga
    11641 ctgacggtag cggcgcggcg gtgcaggaag gtcagggcga tctgtgggtg aaacgtccaa
    11701 acttattcaa ctggcatatg acacaacctg atgaaagcat tctggtttct gacggtaaaa
    11761 cactgtggtt ctataacccg ttcgttgagc aagctacggc aacctggctg aaagatgcca
    11821 ccggtaatac gccgtttatg ctgattgccc gcaaccagtc cagcgactgg cagcagtaca
    11881 atatcaaaca gaatggcgat gactttgtcc tgacgccgaa agccagcaat ggcaatctga
    11941 agcagttcac cattaacgtg ggacgtgatg gcacaatcca tcagtttagc gcggtggagc
    12001 aggacgatca gcgcagcagt tatcaactga aatcccagca aaatggggct gtggatgcag
    12061 cgaaatttac cttcaccccg ccgcaaggcg tcacggtaga tgatcaacgt aagtagaggc
    12121 acctgagtga gcaatctgtc gctcgatttt tcggataata cttttcaacc tctggccgcg
    12181 cgtatgcggc cagaaaattt agcacagtat atcggccagc aacatttgct ggctgcgggg
    12241 aagccgttgc cgcgcgctat cgaagccggg catttacatt ctatgatcct ctgggggcca
    12301 ccgggtaccg gcaaaacaac cctcgctgaa gtgattgccc gctatgcgaa cgctgatgtg
    12361 gaacgtattt ctgccgtcac ctctggcgtg aaagaaattc gcgaggcgat cgagcgcgcc
    12421 cggcaaaatc gcaatgcagg tcgccgcact attctttttg ttgatgaagt tcaccgtttc
    12481 aacaaaagcc agcaggatgc atttctgcca catattgaag acggcaccat cacttttatt
    12541 ggcgcaacca ctgaaaaccc gtcgtttgag cttaattcgg cactgctttc ccgtgcccgt
    12601 gtctatctat tgaaatccct gagtacagag gatattgagc aagtactaac tcaggcgatg
    12661 gaagacaaaa cccgtggcta tggtggtcag gatattgttc tgccagatga aacacgacgc
    12721 gccattgctg aactggtgaa tggcgacgcg cgccgggcgt taaatacgct ggaaatgatg
    12781 gcggatatgg ccgaagtcga tgatagcggt aagcgggtcc tgaagcctga attactgacc
    12841 gaaatcgcag gtgaacgtag cgcccgcttt gataacaaag gcgatcgctt ttacgatctg
    12901 atttccgcac tgcataagtc ggtacgtggt agcgcacccg atgcggcgct ctactggtat
    12961 gcgcgaatta ttaccgccgg tggcgatccg ttatatgtcg cgcgtcgttg cctggcaatt
    13021 gcttccgaag atgtcggcaa tgccgaccca cgggcgatgc aggtggcaat tgcggcctgg
    13081 gattgcttta ctcgcgttgg cccggcggaa ggtgaacgcg ccattgctca ggcgattgtt
    13141 tacctagcct gcgcgccaaa aagcaacgct gtctacactg cgtttaaagc cgcgctggcg
    13201 gatgctcgcg aacgtccgga ttatgacgtg ccggttcatt tgcgtaatgc gccgacgaaa
    13261 ttaatgaagg aaatgggcta cgggcaggaa tatcgttacg ctcatgatga agcaaacgct
    13321 tatgctgccg gtgaggttta cttcccgccg gaaatagcac aaacacgcta ttatttcccg
    13381 acaaacaggg gccttgaagg caagattggc gaaaagctcg cctggctggc tgaacaggat
    13441 caaaatagcc ccataaaacg ctaccgttaa tgttatcgtt gcggtaatgt tgttactgta
    13501 tccctgtggt cgcaggctgt ggccacatct cccatttaat tcgataagca caggataagc
    13561 atgctcgatc ccaatctgct gcgtaatgag ccagacgcag tcgctgaaaa actggcacgc
    13621 cggggcttta agctggatgt agataagctg ggcgctcttg aagagcgtcg taaagtattg
    13681 caggtcaaaa cggaaaacct gcaagcggag cgtaactccc gatcgaaatc cattggccag
    13741 gcgaaagcgc gcggggaaga tatcgagcct ttacgtctgg aagtgaacaa actgggcgaa
    13801 gagctggatg cagcaaaagc cgagctggat gctttacagg ctgaaattcg cgatatcgcg
    13861 ctgaccatcc ctaacctgcc tgcagatgaa gtgccggtag gtaaagacga aaatgacaac
    13921 gtcgaagtca gccgctgggg caccccgcgt gagtttgact ttgaagttcg tgaccatgtg
    13981 acgctgggtg aaatgcactc tggcctcgac tttgcagccg cagttaagct gaccggttcc
    14041 cgctttgtgg taatgaaagg gcagattgct cgcatgcacc gcgcactgtc gcagtttatg
    14101 ctggatctgc ataccgaaca gcatggctac agtgagaact atgttccgta cctggttaac
    14161 caggacacgc tgtacggtac gggtcagctg ccgaaatttg ctggcgatct gttccatact
    14221 cgtccgctgg aagaagaagc agacaccagt aactatgcgc tgatcccaac ggcagaagtt
    14281 ccgctgacta acctggtgcg cggtgaaatc atcgatgaag atgatctgcc aattaagatg
    14341 accgcccaca ccccatgctt ccgttctgaa gccggttcat atggtcgtga cacccgtggt
    14401 ctgatccgta tgcaccagtt cgacaaagtt gaaatggtgc agatcgtgcg cccggaagac
    14461 tcaatggcgg cgctggaaga gatgactggt catgcagaaa aagtcctgca gttgctgggc
    14521 ctgccgtacc gtaaaatcat cctttgcact ggcgacatgg gctttggcgc ttgcaaaact
    14581 tacgacctgg aagtatggat cccggcacag aacacctacc gtgagatctc ttcctgctcc
    14641 aacgtttggg atttccaggc acgtcgtatg caggcacgtt gtcgcagcaa gtcggacaag
    14701 aaaacccgtc tggttcatac cctgaacggt tctggtctgg ctgttggtcg tacgctggtt
    14761 gcggttatgg aaaactatca gcaggctgat ggacgtattg aagtaccaga agttctgcgt
    14821 ccgtatatga acggactgga atatattggc taatacccaa tttttctgaa tctaaaaagc
    14881 gcctgcgggc gctttttttg tctccctttg ataccgaaca ataattactc ctcacttaca
    14941 cgtaatacta ctttcgagtg aaaatctacc tatctctttg attttcaaat tattcgatgt
    15001 atacaagcct atatagcgaa ctgctataga aataattaca caatacggtt tgttactgga
    15061 atcaatcgtg agcaagcttg agtgagccat tatgaaaacg aaaatccctg atgcggtatt
    15121 ggctgctgag gtgagtcgcc gtggtttggt aaaaacgaca gcgatcggcg gcctggcaat
    15181 ggccagcagc gcattaacat taccttttag tcggattgcg cacgctgtcg atagcgccat
    15241 tccaacaaaa tcagacgaaa aggttatctg gagcgcctgt acagttaact gtggtagtcg
    15301 ctgcccgcta cgtatgcacg tcgtggacgg tgaaatcaaa tatgtcgaaa cggacaatac
    15361 cggcgatgac aattacgacg gcctgcacca ggttcgcgcc tgtctgcgtg ggcgttccat
    15421 gcgtcgccgt gtctacaatc cggaccgcct gaaatatccg atgaaacgag tcggggcgcg
    15481 cggtgaaggc aaattcgagc gcattagctg ggaagaagcc tacgacatca tcgcgaccaa
    15541 tatgcagcgc ctgatcaaag agtacggcaa cgagtctatc tatctgaact atggcaccgg
    15601 tacgctgggc ggcaccatga cccgctcctg gccgccggga aataccctgg tcgcgcgcct
    15661 gatgaactgc tgcggcggtt atcttaacca ttacggtgac tactcttctg cgcaaattgc
    15721 ggaaggtttg aactatacct acggcggctg ggcagatggt aacagcccgt cggatatcga
    15781 aaacagtaaa ttggtagtgc tgtttggtaa taacccaggc gaaacgcgaa tgagtggcgg
    15841 tggggtgact tactatcttg aacaggcacg ccagaaatct aatgcccgca tgatcatcat
    15901 cgatccgcgc tataccgaca ccggtgccgg gcgcgaagat gagtggatcc ctattcgtcc
    15961 aggaacagat gccgcactgg ttaacggtct ggcgtatgtc atgatcaccg aaaacctggt
    16021 ggatcaggca ttccttgata aatattgcgt tggctacgat gagaaaaccc tgcccgctag
    16081 cgcgccgaaa aatggccact ataaagctta tattctgggt gaagggccag atggcgtggc
    16141 taaaacaccg caatgggcct cgcaaatcac cggtatcccg gcagagaaaa tcattcaact
    16201 ggcacgggaa atcggcagta ccaaaccggc gtttatcagc cagggatggg ggccacagcg
    16261 ccacgccaac ggcgaaatcg cgactcgcgc tatctcgatg ctggcgattc tgaccggtaa
    16321 cgtcggcatt aacggcggta acaccggcgc gcgtgaaggt tcctatagcc tgccgtttgt
    16381 ccgtatgccg accctcgaaa acccgatcca gaccagcatt tctatgttta tgtggaccga
    16441 tgccattgaa cgtggcccgg aaatgacggc gctacgtgat ggtgtgcgcg ggaaagataa
    16501 gctggatgtg ccgatcaaaa tgatctggaa ctatgccggt aattgcctga ttaaccagca
    16561 ttctgaaatc aaccgtaccc atgaaatcct tcaggatgat aagaagtgcg tgctgattgt
    16621 ggttatcgac tgccacatga cctcatcggc gaaatatgct gacatcctgc tgcctgactg
    16681 caccgcttcc gaacagatgg actttgcact ggatgcatcc tgcgggaata tgtcttacgt
    16741 gattttcaac gatcaggtga ttaaaccgcg ctttgaatgt aaaaccatct atgaaatgac
    16801 cagcgaactg gcaaaacgtc ttggcgttga gcaacagttt actgaaggcc gtacacagga
    16861 agagtggatg cggcatctgt atgcccagtc gcgggaagcg attcctgaac tgccaacgtt
    16921 tgaagagttc cgcaagcagg ggatctttaa aaagcgcgac ccacaagggc atcacgttgc
    16981 ttataaagcc ttccgtgaag atccgcaggc aaacccactg actacgccat cgggcaaaat
    17041 tgagatttat tcgcaggcgc tggctgacat tgccgctacc tgggaattgc cagaaggcga
    17101 tgtgatcgat ccactgccga tctacacgcc gggctttgaa agttatcagg atccgctgaa
    17161 caaacagtat ccgctgcagc ttacgggttt ccactataaa tctcgcgttc actcaactta
    17221 cggcaacgtt gatgtgctga aagcggcttg ccgtcaggaa atgtggatca acccgcttga
    17281 tgcgcaaaaa cgcggtatca acaacggcga taaagtcagg atctttaacg atcgtggtga
    17341 agttcatatt gaggcgaaag tgacgccacg aatgatgccg ggtgtggtcg cactgggtga
    17401 aggtgcctgg tatgacccgg atgcaaaacg tgtcgataag ggcggttgta ttaacgtact
    17461 gaccactcaa cgtccgtctc ctctcgctaa ggggaatccg tcacatacaa accttgttca
    17521 ggttgaaaag gtgtaaggag taaccgatga caacccagta tggatttttt attgattcca
    17581 gccgttgcac cggttgcaaa acctgcgagc tggcctgtaa agactacaaa gatttgacgc
    17641 cagaagtcag cttccgccgc atttatgaat atgctggcgg cgactggcag gaagataacg
    17701 gtgtctggca ccagaacgtg tttgcctact atctgtcgat ttcatgtaac cactgcgaag
    17761 atccggcttg tactaaagtc tgcccgagcg gtgcgatgca taaacgcgaa gatggttttg
    17821 ttgtggtcga tgaagatgtg tgcattggct gccgctactg ccatatggct tgcccgtatg
    17881 gcgcaccgca atataacgaa acgaaaggcc atatgaccaa atgcgatggt tgttatgacc
    17941 gtgttgcgga gggtaaaaag ccgatctgtg ttgaatcttg tccgctgcgg gcgctggatt
    18001 tcggccctat cgacgagctg cgtaaaaaac atggcgatct ggcggccgtt gcgccgttgc
    18061 cgcgagctca ctttaccaaa ccgaatattg tgatcaaacc caatgccaat agccgcccga
    18121 ccggggatac cactggctat ctggcaaacc cgaaggaggt gtgagatggg aagtggatgg
    18181 catgaatggc cgctgatgat cttcacggtc ttcgggcaat gtgtagcagg tggttttatc
    18241 gttctggctt tggcgctgct caaaggcgac ctgcgagcag aagcccagca gcgtgttatc
    18301 gcctgcatgt ttggtttatg ggtgctgatg ggcattggct ttatcgcctc tatgctccat
    18361 cttggttcac caatgcgcgc ttttaactcg ctcaaccggg taggggcttc agcactcagt
    18421 aacgaaatcg ccagcggttc gatctttttt gccgtaggcg gcatcggctg gttgctggca
    18481 atgctgaaaa agctgtcacc ggcattgcgt acgctgtggc tgatagtgac gatggttctt
    18541 ggcgtcatct ttgtctggat gatggtgcgt gtgtataaca gcattgatac cgttccgacc
    18601 tggtacagca tctggacgcc gatgggcttc ttcctgacga tgtttatggg cggcccgtta
    18661 ctgggttacc tgttgttgag tctggctggc gtcgatggct gggcgatgcg tctgctgcca
    18721 gcaatttctg tactggcact ggtagtgagt ggcgtggtgt cagtgatgca gggcgcagag
    18781 ctggcgacta ttcatagttc tgtgcagcag gccgcagcgc tggtgccgga ctatggtgcg
    18841 ctgatgtcct ggcggatcgt gcttttggcc gttgccctgt gcttgtggat tgcaccacag
    18901 ctaaaaggtt atcagcctgc ggttccgcta ctatccgtct cattcattct gcttctggca
    18961 ggggaattaa tcggtcgcgg cgtattctat ggtttgcata tgaccgtggg gatggccgtc
    19021 gcaagctaat cagtaacaac cggggtttcg gccccggttc tcttttattt ctgcttcgtt
    19081 aacgtgtcat aactggtcat caaattacga taatcaggaa tgtggttaga gaacaacgtc
    19141 gccagtcctt caatatcatt acgccagtcg cgatgcagct cgcacgccac gccgaaccat
    19201 gtcatcaatt gcgcaccagc ttgcgacatg cgatcccatg ccgaatgccg ggtaatttca
    19261 ttaaaggtcc ctgacgcgtc agtcacgaca aagacgtcaa acccttcttc aatggctgaa
    19321 agcgccggga atgccacgca aacttcggtt accacaccgg caataattaa ctgtttttta
    19381 cctgtcgctt tgacagcttt tacaaaatct tcgttatccc aggcgttaat atttcccggg
    19441 cgagcaatgt aaggtgtatc ggggaattgt gctttcagtt ctggaactaa tgggccgtta
    19501 ggaccggttt caaaactggt ggtaagaata gttggtaagt tgaaatactt agccaggtca
    19561 cccagcgcca gcacgttatt tttaaactta tcaggttcga tatcccgtac aagggaaagt
    19621 aaaccagcct ggtgatcaac aagcaaaacg gcagcatcat ttttatcaag acgaacatac
    19681 ggtttggtca tcctcttctc ctttcgagag tcagtggtac gtctgaaatc gatcagacgc
    19741 gatgcattgc tctgaaagca tagacgggaa atatgagttt gctgtgacca tgaaattttt
    19801 cgactgaacg aagcgtatga aatttgtgtt agttcaataa aaacaatcag atgagactaa
    19861 tcatctcttc agcattagcg ttatgaatga gtaatataaa tcgataatac cgccgctggg
    19921 atatcgcgta ttttcaccca ttgacaatgt ttttggcggt ggcatgatgc gcatgaaatt
    19981 tgaacttcct cacggtttta attcatgtcc acgtataccc ggcctgtcat gcttttgctg
    20041 tctggcctgc ttttgttgac tctggcgatt gcggtgttaa atacactcgt gccgctttgg
    20101 ctcgcccagg aacacatgtc cacatggcag gtaggcgttg tcagctcatc ctattttacc
    20161 ggcaaccttg tcggtacatt gctgacaggg tatgtcatta agcgcattgg ctttaaccgc
    20221 agctattatc tggcctcctt catttttgcc gctggctgtg ccggccttgg cctgatgatt
    20281 ggattctgga gctggttggc ttggcgtttt gtcgcgggcg tcggctgtgc catgatttgg
    20341 gtggttgttg agagcgcgct gatgtgcagt gggacgtcac gtaaccgtgg gcgtttgctt
    20401 gctgcgtata tgatggttta ttacgtggga acgtttttag gccagttact ggtcagcaaa
    20461 gtttcaaccg agctgatgtc cgtattgccg tgggttacag gtttgacgtt ggcagggatc
    20521 ttaccgctgt tgtttacgcg tgtgctgaat cagcaggctg aaaaccatga ttcgacgtca
    20581 attacgtcaa tgctaaaact ccgtcaggcg cggcttggcg tgaatggctg cattatctca
    20641 ggaatcgttc tgggatctct atatggcctg atgccgctgt acctcaatca caaaggggtg
    20701 agcaatgcca gcattggttt ctggatggcg gtactggtca gtgcgggtat ccttggacaa
    20761 tggccgattg gacgtctggc ggataagttt ggtcgactgc tggtgttgcg tgttcaggtc
    20821 tttgtcgtca ttctcggcag tatcgcgatg cttagccagg cggcgatggc cccagcgtta
    20881 ttcatcctcg gtgccgctgg ctttacgcta tatccggtgg cgatggcatg ggcttgcgag
    20941 aaagttgaac atcatcaact ggtggcgatg aaccaggcct tactgttgag ctatactgtg
    21001 ggaagtctgc ttggcccgtc atttaccgct atgctaatgc agaatttctc cgataattta
    21061 ttgtttatca tgatcgccag cgtatcgttt atctatttgc tgatgctgct gcgcaacgcc
    21121 ggtcatacgc cgaaacccgt tgctcacgtg taaatgaatt caagcagagt gtgaacttac
    21181 tgtttcacac tctgcttttt tgtttcttct atctgacttg ctttattcca aattttattc
    21241 gtttaaaaat aaaatgtgca gcaggttata attttgcatt tcgctatttc cgcacttctt
    21301 atttgccgcg cataatccct cgttttacag atgccccttt aattttggcg aaggatttgt
    21361 ctatggctgg gaatgttcag gaaaaacagc tgcgatggta caacattgcg ctgatgtctt
    21421 ttatcactgt ctggggtttt ggcaacgttg ttaataacta cgccaaccag gggctggtgg
    21481 ttgttttttc atgggtgttt atctttgcgc tctatttcac accttatgcg ctaattgttg
    21541 gtcagttagg ctcgaccttc aaagatggga ggggcggggt cagtacctgg attaaacaca
    21601 cgatgggacc cggactggct tacctcgccg cgtggaccta ctgggtagtg catattccct
    21661 atctggcaca aaaaccccag gcaatactga ttgcgctcgg ttgggcgatg aaaggcgacg
    21721 gttcgttaat caaagaatat tcagtcgtag cgttacaggg gttaacgctg gtgctgttta
    21781 tcttctttat gtgggttgct tcacgcggta tgaaatcgct gaaaatcgtc ggttctgtgg
    21841 cagggattgc gatgtttgtt atgtcgctcc tgtatgtggc gatggcggta accgcgcctg
    21901 caattactga agtgcatatt gcgaccacaa acattacctg ggaaacgttc attcctcata
    21961 tcgactttac ctacattacc actatttcaa tgctggtttt cgcggttggc ggagcagaga
    22021 agatttctcc ttacgttaat caaacgcgca acccaggaaa agaatttcca aaagggatgt
    22081 tatgcctggc ggtgatggtt gcggtttgtg ccattctggg ctcgctggcg atggggatga
    22141 tgtttgattc gcgtaatatc ccggatgact taatgaccaa cggtcagtat tacgcctttc
    22201 agaagctggg cgagtattac aacatgggta atactttaat ggtgatttac gccattgcga
    22261 ataccctggg acaagtagcg gcgctggtat tctcgattga tgccccgctt aaagtgctat
    22321 taggtgatgc tgacagcaaa tatattccag ccagtttatg tcgtaccaac gcttctggta
    22381 cgcccgttaa tggctatttt ctgaccctgg tactggtggc gattctgatt atgctgccga
    22441 ctctcggcat tggtgatatg aacaatctct ataaatggct gttgaacctt aattcggtag
    22501 tgatgccgct gcgttattta tgggtatttg ttgcatttat tgcagtcgtt cgcctggcgc
    22561 agaaatataa accagaatat gtctttattc gtaataagcc tctggcaatg accgtcggga
    22621 tttggtgttt tgcctttacc gcctttgcct gtttgacggg gatcttcccg aaaatggaag
    22681 ccttcactgc agagtggacc ttccagttgg cgctgaatgt tgcaacgccg tttgtgctgg
    22741 taggattagg actgattttc ccgctgctgg cgcgtaaagc gaatagtaaa taattattgt
    22801 ggtcggtgac gctgactctg gcagatctga cccgttttta cataatcgag aaatgcgcgc
    22861 aagccgcagg acatatactg gcggtttgga taatagatct ggaagcctgg acgctctgtg
    22921 ctccagtctt ccagtacaca ttctaaacga ccagtatcaa ggtactcctt gatctgttca
    22981 tagagcagat acccgatacc agcacccatc aaaacggcct ccagttcagc atcaacatca
    23041 tccagaatga tatttcctgc gacagcaatt tccagctcct ttgcaaattg gtttgccgct
    23101 gggataacga aaaacaacac attgatgatt aagcagatca tgaggatggc ggggtttgcc
    23161 atatcttgca aagtactctg gcgttgctgc tacacataat ttcactggcg ggccgatcgc
    23221 cacgctgatc atatcttttt cgacgatgcc actaagtcga acgccagcgt caaagccctg
    23281 ttggacgata tcgacaagag agtcgtcagt tgtgagctcg actttaatat caggatattc
    23341 acgtgtgaaa ccaaccagta aaggcattaa aaatatacgt gcagctaccc gggcggcatt
    23401 gattttgagc gttcctgtcg gtgtcagacg aaaatcattc atctcatcta acatgatctg
    23461 aatttcgtcg aaagctgggc gcaatcgttc atagagatta gaacccgctt ccgttaacga
    23521 tacgcttcgg gttgtccgat tgaataagcg aattttaaga cgttgttcca gtgttttaat
    23581 actatggcta atggcggacg aggataagcc taactcatcg cccgctgcac gaaagctttg
    23641 attacgggcc acggcaaaga aagtggcaaa gtcagacata tccgcattag tgaaaatcct
    23701 tcattaactc atcctgaatc atcacgttta caggattatg ccgcctgcgt aaagtgcgct
    23761 ccagaactta acgtggaggt aaaattatgc agtctgaacg tatttatttg gtatgggccc
    23821 atcctcgtca tgattcattg accgcacata ttgctgatgc gatccatcag cgggcaatgg
    23881 agcggaaaat acaggtgacg gaactcgatt tatatcggcg taatttcaac ccagtgatga
    23941 cgccggaaga tgaaccagac tggaagaata tggataaacg ttattctcca gaggttcatc
    24001 agctttattc agagctgctt gaacatgaca cgttagtggt ggtttttcct ctctggtggt
    24061 acagcttccc ggcaatgcta aaaggatata ttgacagagt atggaataat gggctggctt
    24121 atggagatgg gcacaaatta ccattcaata aagttcgttg ggtggcgctg gttggaggag
    24181 acaaagaatc atttgtccag atgggctggg aaaaaaatat aagcgattat ttaaaaaata
    24241 tgtgcagtta tcttggtatt gaagatgccg atgtcacttt cttgtgtaat acagtggtat
    24301 tcgatgggga agaacttcac gcgagctatt atcagtcgtt attatctcag gtacgggata
    24361 tggtagatgc actataagat gtgttaaaaa cgctgtagca gaatgaagcg cggaataaaa
    24421 aagcggcaac tcaataaagt tgccgcttta cggggaaatt agaacattac cttatgaccg
    24481 tactgctcaa gaatgccttt cacgcgttcc atggtctctt tcttcggtgg tttaacaccg
    24541 tcgagtttgt actcttcacc cattgccacc catttgtgtt tgcccagctc gtggtagggg
    24601 agaagctcga ttttctcaac gttgcccata tcacgggtaa attcaccgag gcgatgcgct
    24661 gaatcgtcat cgtcagacca gcctgggaca acaacgtagc ggatccacac cttcacattt
    24721 ttgttcgcca gatatttagc gaactccagc gtgcggtggt tggaaactcc aaccagattt
    24781 tggtggatct cgtcgttcat ctgtttgaga tcgagcatta ccaggtcggt tacttccagc
    24841 agttcatcaa tcaccggatc gtaacgacga acaaaaccgt tggtgtccag acaggtatga
    24901 atgccttctt ttttgcaggc gcggaaccag tcacgaacaa actcagcttg caggattgct
    24961 tcaccgccgg atgcggtaac gccgccgccg gaagcgttca taaagtggcg ataggtcacc
    25021 acttccttca tcaaatcttc aacggtaact tctttaccgc catgcgtgtc ccaggtgtcg
    25081 cggttatgac aatacaggca gcgcatcagg cagccctgga aaaaggtgat aaagcgaata
    25141 cctgggccgt ctacggttcc acaggattca aaggagtgaa tgcgaccaat aactgacatt
    25201 gcggtgtttc tccagatgtg gcccatctga ggccgtgttg gtgcgcagct cgaaggctac
    25261 gtcgagtctg ttttggcagt caccttaaag tatagatagc tgacaaaaaa ggctctcgtg
    25321 ctaaaaaagg ccccactttc gtggagcctt tattgtacgc tttttactgt acgatttcag
    25381 tcaaatctaa ttacatagat tgagtgaagg tacgagtaat aacgtcctgc tgctgttctt
    25441 tagtcagcga gttgaaacgt actgcgtagc cagatacacg gatggtcagc tgcggatatt
    25501 tttccgggtt ttccatcgcg tcgagcagca tttcacggtt catcacgtta acgttcaggt
    25561 gctgaccacc ttcgatggat gcttcgtggt ggaagtaacc atccatcaga cctgccaggt
    25621 tggtcttacg aacttcgtcg tctttaccca gtgcgttcgg aacgatagag aaggtgtagg
    25681 agataccatc tttagcgtaa gcaaacggca gtttagcaac ggaagtcaga gacgctacag
    25741 cacctttctg gtcacgaccg tgcatcgggt tagcacccgg tccgaacggc gcgccagcac
    25801 gacgaccgtc tggggtgtta ccagttttct taccatacac aacgttagaa gtgatggtca
    25861 gaacagactg agtcgggata gcgtcacggt aggtgtgcag tttctgaatt ttcttcatga
    25921 aacgttctac caggtcaaca gccaggtcat ctacacgcgg atcattgtta ccaaactgcg
    25981 ggtattcgcc ttcgatttcg aagtcgatag ccagaccgtc ttcgtcacga atcggtttaa
    26041 ctttcgcata tttgattgca gacagggagt cagcagcaac ggacagacca gcgataccac
    26101 acgccatggt gcggataacg tcacggtcgt gcagcgccat cagagaggct tcgtagctgt
    26161 acttgtcgtg catgtagtgg atgatgttca gtgcagtgat gtactgttta gccagccagt
    26221 ccatgaagtg atccatgcgc tccatcactt catcgtagtt caggagatcg cctttgatcg
    26281 gttcagactt cggaccaacc tgcattttca gtttttcgtc aacgccgccg ttgattgcgt
    26341 acagcatggt tttcgccagg tttgcacgcg caccgaagaa ctgcatttgt ttaccaacga
    26401 tcatcgggct tacgcagcaa gcgatagcat agtcatcgtt gttgaagtcc ggacgcatca
    26461 ggtcatcgtt ctcatactgc agagaagagg tgtcgatgga cactttagcg gcgaatttct
    26521 tgaagttcag cggcagtttt tcagaccaca gaatggtcat gttcggttcc ggagacggac
    26581 ccatggtgta cagggtgttc aggaaacgga agctgttttt ggtaaccagg gtacgaccgt
    26641 cgaggcccat accaccgata gattcggttg cccagatcgg gtcgccagag aacagttcat
    26701 cgtattccgg agtacgcagg aagcgaacca tacgcagttt catgaccagg tggtcaacca
    26761 tttcctgcgc ttcttgttcg gtgatcttgc cagctttcag gtcacgttcg atgtacacat
    26821 ccaggaaggt ggaggtacga ccgaaggaca ttgcagcacc gttctgagac ttaacagcag
    26881 ccaggtagcc gaagtaagtc cactggatag cttcctgagc gttggtagcc ggaccagaga
    26941 tgtcgtagcc gtatttcgca gccatttctt tcatctgacc cagagcgcgg tgctgttcag
    27001 cgatttcttc gcgcagacgg atagtctgtt ccaggtttac gccgttttcc agatcagtct
    27061 gcagagaggt gaactgagcg tatttgtctt tcatcaggta gtcgataccg tacagcgcaa
    27121 cgcgacggta gtcaccgatg atacggccac ggccataagc atctggcaga ccggtcagaa
    27181 caccggattt acggcaacgc aggatgtccg gagtgtaaac gtcgaacacg ccctggttgt
    27241 gagttttacg gtattcagtg aagatttttt tgatcatcgg gtccagttcg cggttgtacg
    27301 ctttgcagga accttcgatc attttgatac caccgaacgg gataagagca cgtttcagcg
    27361 gagcttcagt ctgcagacca acaacttttt ccaacgcttt gttgatgtaa ccagcgtcgt
    27421 gagaggtgat ggtagaagca acagctgtgt caaagtcaac tggcgcgtga gtgcggtttt
    27481 ccagtttaac gccttccatt actttgtccc acagggtggt ggtcgcttca gtagcgccag
    27541 ccaggaagga ctcgtcaccc tcgtacggag tgtagttttt ctgaatgaag tcacggacgt
    27601 ttacttcatt ctgccagtca cctttggtaa aaccttccca ggctgtggct aacttttcat
    27661 taagctcgga catgtaacac ctaccttctt aagtggattt tttatttact gcgtacttcg
    27721 acaaccatta atggtgatcg ttttcacgca ggtaaatgac ccagtatgtc aacccaacca
    27781 acaaaccacc gccgataata ttaccgatcg taaccggaat caggttatca gtgatgaagt
    27841 tcatcacggt caggtgagaa aaattttccg gtgcagaacc gacagcggtc cagaattccg
    27901 gagatgcgaa gtcgcggatt acaataccca tcgggatcat aaacatgttt gcgatactgt
    27961 gctcaaaacc gctggcaaca aacatcgcga ccggcagcac cataatgaac gctttgtcca
    28021 tcaggctacg gccagaataa ctcatccata ctgccagaca taccatcagg tttgccagga
    28081 taccaaggca gacggcctca ataaaagtat ggtgcacttt gtggtcggcg gtttgtagga
    28141 cgtttagtcc ccattgacca tttgcggtca tatactcgcc ggaaagccac attaaaagta
    28201 caaacagcag tgcgccgacc aggttgccaa aatagacatt tagccagttt ttcgccaact
    28261 gaccccaggt gatgcgccca ctcgccttag caacaacaat caacactgtg gaagtaaaga
    28321 gatcggctcc gcagacaaca caaagaatca gccccagaga gaagcaaatg ccgccaacca
    28381 gttttgccat gccgaagggc attgtgcctg tgccagtggt tgctgtgata tagaagacga
    28441 atgcgattga gatgaaaaca ccggcggtaa tcgccagata gaaagtctta agcggatgtt
    28501 tcgttgcttt atagacaccc gcctcttcgg ccactttggc cattgcagca ggaagtaaaa
    28561 gatcaaaagg gttgtcagct ttcacactaa ctctctcttt attaagtcgg cgacgagata
    28621 ctaacaaagc attatagatg agaaattgat atagatcata tctcgcctgg cttataggcc
    28681 cgtaactcg
//
LOCUS       DAJOWB010000052        28216 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJOWB010000052 DAJOWB010000000
VERSION     DAJOWB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.052"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..1344
                     /gene="gdhA"
                     /locus_tag="OHO85_002441"
     CDS             1..1344
                     /gene="gdhA"
                     /locus_tag="OHO85_002441"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCQ2671802.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLERQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(1461..2501)
                     /locus_tag="OHO85_002442"
     CDS             complement(1461..2501)
                     /locus_tag="OHO85_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCQ2671803.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGG
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(2629..4590)
                     /gene="topB"
                     /locus_tag="OHO85_002443"
     CDS             complement(2629..4590)
                     /gene="topB"
                     /locus_tag="OHO85_002443"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCQ2671804.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRALL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(4595..5638)
                     /gene="selD"
                     /locus_tag="OHO85_002444"
     CDS             complement(4595..5638)
                     /gene="selD"
                     /locus_tag="OHO85_002444"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCQ2671805.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(5755..6306)
                     /locus_tag="OHO85_002445"
     CDS             complement(5755..6306)
                     /locus_tag="OHO85_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCQ2671806.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6467..8323
                     /gene="sppA"
                     /locus_tag="OHO85_002446"
     CDS             6467..8323
                     /gene="sppA"
                     /locus_tag="OHO85_002446"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCQ2671807.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSSSDTKETAGRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKDFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITKALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDTFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            8490..9506
                     /gene="ansA"
                     /locus_tag="OHO85_002447"
     CDS             8490..9506
                     /gene="ansA"
                     /locus_tag="OHO85_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCQ2671808.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDEYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            9517..10158
                     /gene="pncA"
                     /locus_tag="OHO85_002448"
     CDS             9517..10158
                     /gene="pncA"
                     /locus_tag="OHO85_002448"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCQ2671809.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(10251..11609)
                     /locus_tag="OHO85_002449"
     CDS             complement(10251..11609)
                     /locus_tag="OHO85_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2671810.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(11726..12484)
                     /locus_tag="OHO85_002450"
     CDS             complement(11726..12484)
                     /locus_tag="OHO85_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCQ2671811.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(12621..13601)
                     /gene="ydjG"
                     /locus_tag="OHO85_002451"
     CDS             complement(12621..13601)
                     /gene="ydjG"
                     /locus_tag="OHO85_002451"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCQ2671812.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALIINLSDADATLMREMAEALER"
     gene            complement(13611..14558)
                     /locus_tag="OHO85_002452"
     CDS             complement(13611..14558)
                     /locus_tag="OHO85_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCQ2671813.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFSRFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KTRQMIICADMIKPRLNETLDDICEALSYVDYLFPNYAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(14563..15399)
                     /locus_tag="OHO85_002453"
     CDS             complement(14563..15399)
                     /locus_tag="OHO85_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCQ2671814.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMARKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKEKQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            complement(15420..16463)
                     /locus_tag="OHO85_002454"
     CDS             complement(15420..16463)
                     /locus_tag="OHO85_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HCQ2671815.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLIMAEQLGATVVI
                     NGTKEDTIARCQQFTEDMGADIVFETAGSAVTIKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(16480..17859)
                     /locus_tag="OHO85_002455"
     CDS             complement(16480..17859)
                     /locus_tag="OHO85_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2671816.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIVVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            complement(17886..18962)
                     /locus_tag="OHO85_002456"
     CDS             complement(17886..18962)
                     /locus_tag="OHO85_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCQ2671817.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(19332..19604)
                     /locus_tag="OHO85_002457"
     CDS             complement(19332..19604)
                     /locus_tag="OHO85_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCQ2671818.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(19646..20059)
                     /gene="msrB"
                     /locus_tag="OHO85_002458"
     CDS             complement(19646..20059)
                     /gene="msrB"
                     /locus_tag="OHO85_002458"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCQ2671819.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            20401..21396
                     /gene="gapA"
                     /locus_tag="OHO85_002459"
     CDS             20401..21396
                     /gene="gapA"
                     /locus_tag="OHO85_002459"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCQ2671820.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            21480..22364
                     /locus_tag="OHO85_002460"
     CDS             21480..22364
                     /locus_tag="OHO85_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCQ2671821.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(22415..23269)
                     /gene="yeaE"
                     /locus_tag="OHO85_002461"
     CDS             complement(22415..23269)
                     /gene="yeaE"
                     /locus_tag="OHO85_002461"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCQ2671822.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYIGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(23359..24105)
                     /gene="mipA"
                     /locus_tag="OHO85_002462"
     CDS             complement(23359..24105)
                     /gene="mipA"
                     /locus_tag="OHO85_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCQ2671823.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGSAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            24541..26475
                     /gene="yeaG"
                     /locus_tag="OHO85_002463"
     CDS             24541..26475
                     /gene="yeaG"
                     /locus_tag="OHO85_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCQ2671824.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            26588..27871
                     /locus_tag="OHO85_002464"
     CDS             26588..27871
                     /locus_tag="OHO85_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCQ2671825.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNSTAKD"
     gene            28018..>28216
                     /locus_tag="OHO85_002465"
     CDS             28018..>28216
                     /locus_tag="OHO85_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCQ2671826.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLT"
BASE COUNT         7276 a         7229 c         6576 g         7135 t
ORIGIN      
        1 atggatcaga catattctct ggagtcattc ctcaaccatg tccaaaagcg cgacccgaat
       61 caaaccgagt tcgcgcaagc cgttcgtgaa gtaatgacca cactctggcc ttttcttgaa
      121 caaaatccaa aatatcgcca gatgtcatta ctggagcgtc tggttgaacc ggagcgcgtg
      181 atccagtttc gcgtggtatg ggttgatgat cgcaatcagg tacaggtcaa ccgtgcatgg
      241 cgtgtgcagt tcagctctgc catcggcccg tacaaaggcg gtatgcgctt ccatccgtca
      301 gttaacctct ccattcttaa attcctcggc ttcgaacaaa ccttcaaaaa tgccctgact
      361 accctgccga tgggcggtgg taaaggcggc agcgatttcg atccgaaagg aaaaagcgaa
      421 ggtgaagtga tgcgtttttg ccaggcgctg atgactgaac tgtatcgcca cctgggcgcg
      481 gataccgacg ttccggcagg tgatatcggg gttggtggtc gtgaagtcgg ctttatggcg
      541 gggatgatga aaaagctctc caacaatacc gcctgcgtct tcaccggtaa gggcctttca
      601 tttggcggca gtcttattcg cccggaagct accggctacg gtctggttta tttcacagaa
      661 gcaatgctaa aacgccacgg tatgggtttt gaagggatgc gcgtttccgt ttctggctcc
      721 ggcaacgtcg cccagtacgc tatcgaaaaa gcgatggaat ttggtgctcg tgtgatcact
      781 gcgtcagact ccagcggcac tgtagttgat gaaagcggat tcacgaaaga gaaactggca
      841 cgtcttatcg aaatcaaagc cagccgcgat ggtcgagtgg cagattatgc caaagaattt
      901 ggtctggtct atctcgaacg ccaacagccg tggtctgtac cggttgatat cgccctgcct
      961 tgcgccaccc agaacgaact ggatgttgac gccgcgcatc agcttatcgc caatggcgtt
     1021 aaagccgtcg ccgaaggggc aaatatgccg accaccatcg aagcgactga actgttccag
     1081 caggcaggcg tactgtttgc accgggtaaa gcggctaatg ctggcggtgt agcaacgtca
     1141 ggccttgaaa tggcgcaaaa cgctgcgcgc ctgggctgga aagccgagaa agttgacgca
     1201 cgtttgcatc acatcatgct ggatatccac catgcctgtg ttgagcatgg tggtgaaggt
     1261 gagcaaacca actacgtgca gggcgcgaac attgccggtt ttgtgaaggt tgccgatgcg
     1321 atgctggcgc agggtgtgat ttaagttgta aatgcctgat ggcgctacgc ttatcaggcc
     1381 tacaaatggg cacaattcat tgcagttacg ctctaatgta ggccgggcaa gcgcagcgcc
     1441 cccggcaaaa tttcaggcgt ttatgagtat ttaacggatg atgctcccca gggaacattt
     1501 cttatgggcc aacggcattt cttactgtag tgctcccaaa actgcttgtc gtaacgataa
     1561 cacgcttcaa gttcagcatc cgttaacttt ctgcgatagc agcagatatg ccagtaaaga
     1621 aatcccatta gactattttt ttgataatct tcttcgcttt cgaacaactc gtgcgccttt
     1681 cgagaagcaa gcattatata atgccaggcc agttcttctt caattgtccc gttttgaaaa
     1741 gctgtgcttg atatcgaggt catccatgat aattccgccg cccatattag cttcgccgag
     1801 gatttaccgg agcgattatt agcgcaatca gagatatagt ctgagggaaa aacagcaaat
     1861 ttattcaaca aggcgataac ctgctctggg gcttcctcca tgtttgcttt aaaggtattg
     1921 gctccatggt cgccagaaag aaaatgctcc attaaggcac aataactttc gctatcttcg
     1981 ataccccatt gatcctctaa agactcgcgt cttttactta tgatatcgat cgagtcaaag
     2041 ggaagcacat gatattggaa ggtatctttg ccaggttcag gctttcgcgg ccagaactcc
     2101 agcgtttcag accattgctt atgatagaac cgataaggtg cgatcaattg tagcgcctgt
     2161 aacttctcga tactgagcgg ctcaatacct ttagcctgat aataatgcag ttgttctttt
     2221 tttgctttaa aaccggcccg aacaataagc cccatcataa ttaatagata aagaaaagag
     2281 catcccgcag taatcaggcc tctttcattc aaaccgttgg atgttatcgc tgcgaataca
     2341 aacattacag cgacaacaca tgttaaatac cccccccact tacaaagcag catggcctta
     2401 ttttctttaa tcatccgttc aaaattacta ttaaatattt cccagccatt aaaagaatac
     2461 ttctcgctcc caggatggtt ttgtaataaa acttttttca tcacattccc tgttattaca
     2521 tactttagag gcgataattt tatccatgca aaaaaaatat ccaacgaatt ttcttgatcc
     2581 gctggatatt tcagaatatg actcgaatag cacgaaagat tcgctcgctt acgctatcgc
     2641 cccgcttccg gcttcatctg ctggcggact ttttttcgca ctacgtttac gcggtgcagc
     2701 ctttttctta tcagcactgc caccactgcc cggagccaca atgccgcgaa actgccgcac
     2761 cggagtacgt ttggcttgat caataagctg atatagcgtc cccaccagcg gctgcataaa
     2821 gtcctgatag cgacactgct tttcgctgat ttgcgtcagc accgattccc agtgcgcggt
     2881 catgtccggt cgcgtagcca tttctggcag cgaatggaat aacgcttttc cggcgtcggt
     2941 ggagtggata tagcgccctt ttttggtgag gaaaccacgc ttgaacaaca gttcaataat
     3001 cccggcacgc gttgcttctg tccccagacc atcggtcgca cgaaggatct ttttcagatc
     3061 tttgtcctgc acaaagcgcg caatcccggt catcgccgaa agcagtgttg catcggtaaa
     3121 atggcgcggc ggctgggttt gccgctctac cacttcacct ttttcacaca gtaactcatc
     3181 gcctttcgcc accacaggca atggcgtgcc gtcgttttct tcatcgcgct ctttgctgcc
     3241 taacagcgcg cgccaacctg cttcagcaag aaaacgcgct ttagcgacaa atttgccttt
     3301 ggcaatgtcc agttcgataa cacacttgcg gaacaccgca tccgggcaga actgcatcag
     3361 atactgacgg gcaatcaggt tatagacctt cgcttcgttc tccgtcaggt tgatcgcaga
     3421 actccgtgcg gtcggaatga tggcgtggtg cgcatcgacc tttttgtcat cccaacagcg
     3481 gttgcgtata tctggatcta ccactggctg cggcaacaga tctggtgcat gaacgctgat
     3541 ggcattcatc accgcgtggc gtccggcaaa atgttcttct ggcaaatagc gacaatcgga
     3601 acgcggataa gtgattaact tgtgtgtttc gtacagcttc tggcagatat caagcacgtt
     3661 ctgcgcgctc agaccaaagc gttttgccgc ttcaatctgc aacgccgaaa gcgaaaacgg
     3721 cagcggcgcg gattctgatt cccgtttatc gttatagctg gtgacaatag ccggttgacc
     3781 actaatgcgg ttaaccacat gctccgccag tggacgatgt aacaagcgcc cttcttcatc
     3841 ctggtacggt tcacacgctt cgctcggttg ccagatagcg gtaaaccgct catcggcagg
     3901 tgtcacgata tgcgctttga cttcaaagaa gtctttcgcc acgaagtttt caatctcttc
     3961 atcgcggcgc accaccagcc cgagcacggg tgtctgcacg cgtcccacgg aaagtacgcc
     4021 ctgataaccg gcattgcgac cgagaatggt atacgcacgg gtcatattga tgccgtacag
     4081 ccagtcggca cgcgctcgcg ccagcgcaga aacgcacaat ggcacaaact cactgttgga
     4141 acgaagacgg tcgatcgccc gctcaaccgc ctgcgggttc aggtcgttta tcaagcaacg
     4201 ctgcacctgc tggcgctttt ccggtgccag ttgcagatag tccagcactt catccaccag
     4261 caattgccct tcacgatccg ggtccccggc gtgaacgatt tcgctggctt catgcaggaa
     4321 gcgtttgatg acgttaagtt gtttggtcac ggagggtcgg ggctgtaatt gccacttttc
     4381 cgggacaatc ggcaaatccg caagattcca gcgcgcatag cggctgtcgt aggcgtctgg
     4441 ctgcgcctgc tcaagcaggt gaccgataca ccaggtcacc acctgaccat taccgcactc
     4501 gataaagcca tcgcctttac ggtgcggttt gggcaggaca tcagcaatgg cgcgcgccag
     4561 actcggtttt tcggcaataa acaaccgcat tgaattaacg gatctcaacc atggcacgac
     4621 cgccgcgcgc tggcaccagt tcgccaatcg ccgtcagttc aatgccaaac tcggcggctg
     4681 tagttttaac ctcattttct gcttccggca tgaccgccag cagcaaaccg ccagaagttt
     4741 gcggatcgca cagcagatcg cgcacttcac gcggcatttc acccatcaga tgaccgtagc
     4801 tggcaaagtt acgttcggtg ccgccaggca ctgcgcccaa cttaatgtac tcttcaaccc
     4861 ccggcaattt tgggatcgcg tcatagtcaa cgcgcgcctg cacacctgcg ccctgacaca
     4921 tttcgctcaa atggcccagc agaccaaaac cagtaacgtc ggtcatcgct tttacacctt
     4981 cgatgttggc aaaggacgca cctgcgatgt tcatccggca catcacttcc gtcgccagtc
     5041 cctgatgttc tggtttcaac agtgattttt tctcagccgt ggtaagaacg ccgatcccca
     5101 gcggtttcgt caggaacagt ttgcatccgg cttgtgcggt actgtttttc ttcacccgct
     5161 cggtcggtac gatccccgtt accgccagac caaaaatcgg ctccggcgca tcgatggagt
     5221 gaccgccagc cagcgcaata cccgcctgac ggcatgcata gcgtccacct tcggttactt
     5281 cgcgggcaat ttctggggaa agtttgttaa tcggccagcc gaggatcgca atcgccataa
     5341 tcggtttgcc ccccatcgcg aagatatcgc tgatggcgtt agtcgccgca atgcggccaa
     5401 aatcgaaagg attatcaacg atcggcataa agaagtcggt ggtactgata acgctggtgc
     5461 cattgcccag atcgtacacc gccgcatcgt cgcgggtctc attacccaca agcaaattcg
     5521 gatcaacaaa cttcgcctgc tcactgtgca ggatggtttc caacactttt ggggaaattt
     5581 tacagccgca accagctccg tggctgtatt gggtcaaacg aatcgagttc tcgctcatgg
     5641 acatctcctg tcaatgcaat ccgggtatgg taaccctcat tccgtgaagt gataagtgag
     5701 agtgtctgaa ttcctgcgcc tttgctcaca atccagacag tttcgcgaca attatcagaa
     5761 ataagttaca aacggcgtcg ggtccgggac gttaatcgac gtagatgctt tcaactgcgg
     5821 cgtgccgagg tagagaaaac cgacaatttt atcctgctca cggcaaccga atgcttcacg
     5881 cactaccgga ctttcagtta atgcgccact gcgccagatg ccgccaaatc cttgggcaac
     5941 ggctgccatt tgcatcgcca tgaccgcgca tccggcagac atttcctgtt cccatcgcgg
     6001 gactttatga ttttcttcgc atttcgccac caccgtgatg atgagcggtg cgcggaacgg
     6061 cgcattacgg gctttgtcga tagctttgtc atcactaccg gcagcaatcg ccccctgttc
     6121 cagtacggcg ctgaaacgct cgcgcccttc cccttcaatc acaaaaaaat gccacggttg
     6181 catggactta tggtccggcg cacgcatacc cgcacgcagg atgttttgca gttgttcacc
     6241 cgttggcgcg ggttcagcca ggcgggaggc gctacggcga ttgatcaata gttcgagtgc
     6301 atccatttga ttaactcctg tcgtgatatt tattcacaaa attaacacga gagtggattt
     6361 tgttacagca cagtccgcaa ttcctgctga caagtgccgg ttgggtcatt acgataacca
     6421 catctattgc gcctgtgaca ggtgtgacct taagttggga gaatacatgc gaaccctttg
     6481 gcgatttatt gccggatttt ttaaatggac gtggcgtctg ctgaatttcg tccgtgaaat
     6541 ggtacttaac ctgttcttta tttttctcgt actggttggt gtggggatct ggatgcaggt
     6601 cagtagtagc gataccaaag aaaccgccgg tcgtggcgca ctactgctcg atatttccgg
     6661 cgtgatcgtc gataagcccg acagttcgca gcggtttagt aagttaagcc gccaactgct
     6721 tggcgccagt tccgatcgtc tgcaggaaaa ctcgctgttt gatatcgtga acacgattcg
     6781 tcaggctaaa gatgaccgga acatcaccgg tatcgtgatg gatttgaaag actttgcggg
     6841 cggcgaccaa ccgtctatgc agtacatcgg caaagccctg aaagagtttc gtgacagcgg
     6901 gaaaccggtt tatgccgtcg gcgagaacta cagccagggt caatattatc tcgccagttt
     6961 cgccaataaa atttggctgt caccgcaagg cgtggtggat ttgcacggtt ttgctaccaa
     7021 cggtctgtac tacaaatcgt tgctggataa gctgaaagtt tccacccatg tgttccgcgt
     7081 gggtacgtat aaatcagccg ttgaaccgtt tattcgtgat gatatgtcac cggcagcccg
     7141 cgaagctgac agccgctgga ttggtgagct gtggcaaaac tatctgaata ctgttgccgc
     7201 taaccggcag atccctgctc agcaggtatt ccctggcgcg caagggttgc ttgagggttt
     7261 aaccaaaacc ggtggcgata ccgcgaaata tgcgctggaa aacaagctgg tcgatgcact
     7321 ggcatcgagt gccgaaatcg aaaaagctct gaccaaggag ttcggctgga gtaagactga
     7381 taaaaattat cgcgccatca gttattacga ttacgcattg aaaacgccgg cagataccgg
     7441 tgacagcatc ggtgtcgtct tcgctaatgg cgcaattatg gatggcgagg aaactcaggg
     7501 gaatgttggc ggtgatacca ctgcggcaca aatccgcgac gctcgccttg acccgaaagt
     7561 gaaagcgatt gtcctgcgtg ttaatagccc aggcggcagc gttaccgcgt ctgaagtgat
     7621 tcgcgctgaa ctggcagcag cccgggcagc gggtaagcct gtggttgtat cgatgggcgg
     7681 catggcggca tctggtggtt actggatttc cacaccagct aattacattg tggctaaccc
     7741 cagcaccctg accggttcta tcggtatctt cggcgtgatc accaccgtag aaaatagtct
     7801 ggattcgatt ggtgttcata ctgatggtgt ctcaacttca ccgctggcgg atgtttctat
     7861 cacaaaggca ctgccgccgg aagcgcagca gatgatgcag ttaagcattg agaatggcta
     7921 taaacgcttt atcacgctgg ttgctgatgc gcgtcattcg acgccggagc agattgataa
     7981 aatcgcccag ggccacgtct ggaccggtca ggatgcaaaa gctaacgggc tggtcgatag
     8041 tctcggggat ttcgatgatg cggtcgccaa agcagcagag ctggcaaaag tgaaacagtg
     8101 gcatctggaa tactacgttg atgaaccgac cttcttcgac aaagtgatgg acaatatgtc
     8161 aggttctgtc cgggcaatgt tgccagatac gttccaggcc atgttacctg caccgctggc
     8221 ctcggtagcc tctaccgtta aaagtgaaag tgacaagctg gccgcgttta acgacccaca
     8281 aaaccgttat gcgttttgcc tgacctgcgc caacgtgcgt taagtcttgt actgagtggc
     8341 cgacagatcg tcggccacat tattttttac gtcgacgaat cctcttcccg ctgtctcgcc
     8401 ccatatttcc ttatccgcat agtatcaggt gcgctccccc tgcctcacgt atatactttt
     8461 gctctttcga tatcattcat atcaatatca tgcaaaagaa atcaatttac gttgcctaca
     8521 cgggcgggac catcgggatg cagcgttccg agcagggtta tataccggtg tcaggtcatc
     8581 tacaacgcca actggcgctg atgccggaat tccatcgccc ggagatgccg gatttcacca
     8641 ttcatgaata tacgccgctg atggattctt ctgatatgac gccggaagac tggcagcata
     8701 ttgctgaaga tattaaagcg cactatgacg agtacgatgg ttttgtcatt ctgcacggca
     8761 ccgacacgat ggcgtatacc gcctctgcgc tgtcgttcat gctggagaac ctcggaaaac
     8821 cggttattgt gacagggtca caaatcccgc tggctgagtt acgctctgac ggacaaatta
     8881 atctgctgaa tgcgttgtac gttgcggcga attatccgat caacgaagta acgctctttt
     8941 tcaataaccg attgtatcgc ggcaaccgca ctaccaaagc ccatgccgat ggttttgatg
     9001 cgtttgcctc tcctaacctt cctccgttac tggaagcagg tatccatatt cgtcgtttga
     9061 atacgccacc cgccccgcac ggtgaagggg aattaatcgt tcatccaatc accccacaac
     9121 caattggcgt agtgacgatt tatccgggga tttctgctga cgtcgtgcgc aattttctgc
     9181 gccaaccggt gaaagcattg attctgcgct cctatggcgt gggtaatgcg ccacaaaaca
     9241 aagccttcct gcaggaatta caagaagcca gcgatcgcgg tattgtggtg gtcaacctga
     9301 cacaatgtat gtccggtaaa gtgaatatgg gtggttatgc caccggtaac gccctcgccc
     9361 atgccggcgt tattggcggt gcagatatga ctgtagaagc cacgctaacc aaactgcatt
     9421 acctgctgag ccaggaactg gatactgaaa ccattcgcaa ggccatgagc caaaacctgc
     9481 gcggcgaact gacgccggat gattaaggag actgcaatgc cccatcgcgc cctgttactg
     9541 gtcgatttac aaaatgattt ctgtgctggt ggcgcgctcg ccgtaccgga aggtgacagt
     9601 acggtggagg ttgctaaccg ccttattgac tggtgccagt cgcgcggaga agcggttatc
     9661 gccagtcagg actggcaccc ggcgaatcac ggcagttttg ccagtcagca cggtgtagag
     9721 ccttatacgc caggccaact cgacggtttg ccacaaacct tctggccaga tcactgtgtg
     9781 cagaacagtg aaggcgcaca attacatccg ttactgaacc aaaaagcgat cgcagcggtg
     9841 ttccataaag gcgaaaatcc tttagttgac agttacagtg ccttttttga taacggccgt
     9901 cggcagaaaa cctctctcga tgactggtta cgcgatcatg aaatcgatga attgatcgtt
     9961 atgggcctgg ctactgacta ttgcgtgaag tttaccgtgc tggacgcgtt acagttaggt
    10021 tataaggtga acgtgattac cgatggttgt cgtggcgtga atatccagcc ccaggacagt
    10081 gcacacgcgt ttatggagat gtcagcagct ggggcaacgc tatatacgct ggcagactgg
    10141 gaagagacac aggggtaatt ttacgctggc ctacaattct gtactggcat tgtaggccaa
    10201 ataaaacacg tcagtggtac atctggcaat tgatgccatc aacgaaagat ttaattcacc
    10261 tcagaaatct cttccagcga caccttccgc gtttcgatgc caaaaatgga gagaaccagc
    10321 gcacagagca ctaacatgac accaagcacc ataaacaccg tgatcgaccc ataatgtgtt
    10381 aataatgccg caacgccata gggcgtgaaa actgcgacga tccgtccgac ggcattaacg
    10441 aaacccgaac cgcgcaggcg taaatgcgtt ggccaaagct ccgggatata aaccgctgac
    10501 gcgaagcaaa catacatgta taaaaagaag atcatcacca gaccatagat taaaatcgcc
    10561 cactctgtag tctgaattga atagatatag cctaacacgg caataataat aagtaaggcg
    10621 gagccaaata atcgacgagg aaaatgatca ataattaatg ccgcaataaa tattcctacc
    10681 ggagcaccaa tcataataac agcggtcatt aatattgatt tatcgacatc aatcccggag
    10741 ttaacaaata tggtcggtat ccatacggta atggtataaa gcgaaatgtt cattgcaatt
    10801 aaaacagtta tcgcaactaa tgtacgtcgt aacatttccc ctttaaacag gagccagaag
    10861 gtacccttga ttaccttaac cttgctgttg ctctggttca aagtaagctg gggtaaacga
    10921 atactcttct ccctttcaat ttgctgctct acttcacgaa gttggctttc tgcaccggcg
    10981 atttgccctt tccctgccag ccatcgtggc gactcgataa agtatttacc tgagagaaac
    11041 caggctaaca gtatgccaat accacccaga agaaacatta ttcgccaact aaaaaaagcg
    11101 ataaccacca cgcctatcgc cgcagacagc atgggcgacc agttaccaac aaatgagagc
    11161 cgcgcggacc attttccacg caccgtcgcg gggataaact ccgtaaatga ggcatagcca
    11221 accataatca gcgcccccat tcctgttccc atcaggaagc gaaagaaaat gagccagtac
    11281 atatcaggga caaaagcggc ccctgttgca gcaataccga cgatgagaag atttatgcga
    11341 aacgccctgc gccgcccaaa gtagtcacca ataaacccac cagtaagtga gccgatgaaa
    11401 taaccaaaca ttaatgccga ggtaaaggcg gcattgagga aattatttga ccatccattg
    11461 cttaccagct ttgctaagac gacattacct gagtaactca aaaaccccgt taataacaga
    11521 ctaaagctta ttataccaaa tatacgataa tgaaaccggg ccaaaggcaa gcggtccagt
    11581 cttgcgccaa tttgatcata ttgttccatt aggtgcctcg gatttgctaa aaaggtagca
    11641 attgcggcaa attcacttgc cgcatcttta tcatcatttt atttcaaggc gcaggaacaa
    11701 ataatattga caacgtgtgc ttaaattacc acaccagctg gatattattg tctttgcaaa
    11761 atgctatcca ctctgcaccc ggtgatttat cagttattat gtaattaata tgactaaaat
    11821 cagctaactg gacaaaagct ttgcgatcaa acttagagtg atcaaccaat aacgcaactt
    11881 ctgtcgcctg acggatcatt gtctttttga tttcagcttc cgcttcatta gagtccagcg
    11941 cgccgctgtt aatatcaaga cctttacaac tcattaccat gatatcgaca tgatagcggc
    12001 tgatgatctc tttagtaatt cttccttgca gtgaaagtgt atttttgttt agttccccac
    12061 ccgttgaaac gactttaatt tctgactgag ccagaacatg aatcgcttcc gccgagttgg
    12121 ttagcaacgt caggtcacta cggtcctgta acaatttgag caattccatt acggtactac
    12181 tggaatcggc tgccatggtg gttttattgt cgataaaggg tagtgccttg cgtgcaataa
    12241 gctgcttctc ttcataaaac gatgaagcgc gcttataaaa atggatattc tccgtcaaca
    12301 tcgctgtatt taaaacagca ccaccatagg ttctggtcaa aaagccttca tcttccagct
    12361 tctcaagatc gcggcgaatg gtttcttcgg ttacctgaaa aatcccactc aaatttgaga
    12421 ctgtcacttt tttatcgttg gcaaccattt gcttaattgc ctgaatcctg tcttttgccg
    12481 ccacgattac acccctgtat ctttttacat cacattagcg cgattatcgc ataaccgatg
    12541 tttactttca aaacaacctg tttgaatcac agattttcat cacagttttc acagaaacag
    12601 aggtgaatcg tgttgagtat ttaacgctcc agggcctctg ccatttccct catcaatgtt
    12661 gcgtctgcat ccgataagtt gataatcagt gccgcgacat tttcgcgtac ctgttccggt
    12721 gcagtagccc cactaagaat ggagattaaa tcactctgtt ttaatatcca cgccagtgcc
    12781 agagtgggaa ttgtgcactg ataacgagca caaagtggct gccactgttc aagcatatca
    12841 atcactttca gcatgttttc acgctggaac cagactttat ttgcccgagc gccgcccgga
    12901 acgtaatcac gagtgatggt gccggtcaac aatccctgct ctagcgggga ataaacctga
    12961 accacaatgc cattatcacg acatagcggc agcagttcgt tttccattgc ccggtcgagg
    13021 atactgtatt tcgcctgaat aatatccagt tcaccatatt gcagatactc gcggatatgg
    13081 tcagcatcga cgttagcagc gcctatagcg cgaattttcc cttcagcctt caactcatta
    13141 agcactgcga cagtttcagc gatcggcgta aaaaatggcg gcaccgactg ccagtgcgtc
    13201 atgtagatat cgatgtaatc aatacccaga cgttgcaagc tggcttctac ctcttcgcgg
    13261 atagattccg gggaaaggtt tttatacaac tgccgatcgc caactttgtt gaataaactt
    13321 ccttttcgtt cccagacaat gccgcatttg gtttctacta caacctgttc acggggcagt
    13381 ttttttaacg cctgaccgac gataacttca ctattgccaa agttatatcc tggcgcagta
    13441 tcaatcagat taatgccgca acgatgggct tcaagaatcg tatcaataca tatttgccga
    13501 tcgagatcgc cattccatgc aggaccgccg ccaatggccc atgtccccaa ccccattcgc
    13561 gaaagcgtaa tatccgttgt gcctaaaggt atctttttca tttgccttca ttatccttcg
    13621 tattcttcca gcaattgttc gaccagcttt ctgtttttta cgccggtggt ggcaccgacg
    13681 cttagaaccg agatagccgc cgttgcattg gcaaagcgtg cgcattcacg cagatttttg
    13741 ccttctaaca gtgccgcaat aaaacctgaa gcaaagttat cgcccgcgcc aatggtgtcg
    13801 atggcggtta ttcctgcgac cgccggcacc ttcatcgtca tgtcaccacg cttgataaag
    13861 cagccgtctt tacccgtttt aatcaccacc gtttttacgc cgcacgcaag aaagcagtca
    13921 gcaatttcat ccaatgtctc tttcccggtg agtaattttg cctcggcata attaggaaac
    13981 agataatcga cataactcaa tgcttcgcaa atatcatcca gcgtttcatt caaccgcggt
    14041 ttgatcatat cggcacagat aatcatctgc cgggttttag cttgcgtaaa aatttctgtt
    14101 agcgctttac catccaatag aggactgttg aaaatactgg ccagagataa taatttcgcc
    14161 tgagaaaacc gcgaaaaatc aacatcgtca atattcaatt tccacagact gccgttacgg
    14221 tttgtgacaa acgtccgctc gccatcctcc gtcaccagtc caacgttaat agaggtatct
    14281 atgctgacgt cctgcttcag gctttgaata tcaatattct ctttgcggca atggtcgaga
    14341 ataaactgtc ctgcggcatc tttaccaata cggctcatta atgctgtacg atggcccaga
    14401 cgagaaataa ttgttgcttc atttattgca tcacccccgg tggtcattgc gattctttca
    14461 agaggataag aatccacatc aaagatattt ttactgaccg gttgcaatgg aatatcaaca
    14521 atagcggcac ctatacaaat aacgtcgaga ttatccatat cattattccg ctttgccatc
    14581 agaaccgaac agtttaattt tttccaatgc ccgttctttt acagctttac gtacttcacg
    14641 ttctaaatgc aggaaaggct gatcctgttt ttctttcact gcgaccattg cggcctgaca
    14701 gagctccgta tgaatgttga ttttcgcgat acccaacgaa attgcagttt taatgtcggc
    14761 atcactgatc cccgatgcac cgtgcaaaac cagcggaaca gaaacggcat cgcgtacgcg
    14821 tttgacgacc tcaaagttca attgtggctc tgacgtataa accccatgct ggttgccgat
    14881 ggcgacagcc aatgagtcac agcccgtacg ttcaacaaat tcagccgcct ggtcaggatc
    14941 ggtatagtga tagcccgcca gcgcttcctc ataaacggtt tcattaccga catgccctaa
    15001 ttccgcctct accggaatac ccagcggatg gaagaaatcg acagcctctt tggttaaacg
    15061 aatattttct tcgaaatcaa atgcagaagc atcacgcatt aatgaattca taccatgagt
    15121 ccaggcgtta tgaataatct ccatactccg accatgatcc caatgagtta ttaccggcac
    15181 agttgctttt cgtgccattg ataccatcat gtgagagaaa tcttcaaatg aggtgttgcc
    15241 gacaaaacct gtaccaaaag aaataataac cggtgatttc gcttcttcgg ctgcgtcgat
    15301 aacgcccatc aacatttctg cattccatac gttaaaatgg gcaattgcat aatgtttatt
    15361 tgtggcatcg ttttcccaat atctgatatc tgcgagcata ttctgttctc ccgtattaat
    15421 taatcgctaa ttttaataac gcctttaata atgtcgcgtt tgttgttaac tgactcttca
    15481 aatgcctgtt gtacatcccg ataatcgtaa atatgcgtca ccatcgattt cacatcgaat
    15541 cgccctgaag aaatagcttc aatcgtgacc ggataacgat tggcatagcg gaataccgtc
    15601 tggatagtga cttcgcgatt gattttgagg aaattgattg ccgaatcgcc cggtacagta
    15661 ccaacaatca taattttacc gccgcgcatt accagataag gtgcctgttt aatggtgacc
    15721 gcagaacccg ctgtttcgaa aacaatatct gcgcccatgt cttcggtaaa ttgctgacag
    15781 cgtgcaatag tgtcttcttt tgtgccgtta ataaccactg tcgcaccaag ttgttccgcc
    15841 attatcagac gtttttccag cacatcaacg acggcaattt ccgttgcgcc caggcatttg
    15901 cacgcttgca acgtcatcaa accaatacaa cctgctccca gaataattat cttcttaccc
    15961 ggtttaacat ctgccagcat cgcggcatgc atcccgactg cggcaggctc caccagcgcc
    16021 ccttccatcg tgtccatatt gtctggcagt ttgtaagtaa agctctccgg atgacacaga
    16081 tagtgcgtta atgcgccgcg gtagttgggt tgtgtcgcca taaaatcaac gtccgggcag
    16141 atgttatatt tgccttccag acagtaacga cagtgaccgc aaggaacgcc aggttcgata
    16201 tttacccgat cccccggttt aaatttgcgc acgcggctac ccacagccac aaccgtcccg
    16261 gcgcattcat gacccaggcc aatttcttga tttgggtctt taggcggaat aaacgggcct
    16321 gattcaaaac catgtacatc tgaaccacaa ataccgacat attctacttt aatcaaaact
    16381 tcatcttctt taggcactgg tatttctgct gaaataattt tcattgtgcc cggcacctgc
    16441 aatattgctt ttgaattttt cattttgaat ccacccgtat tatttattgg ctactgcatc
    16501 aatacttaaa ctttcaacgg agacaccttt ggtttcaatt ccaatggtgg cgatagcaat
    16561 tgcgacaaca attgaaacag cccccagaag aataaagact cccgttacgc cataactact
    16621 gagcagcact gcaacggcat aaggtgcggc aataccactg attcgcccta ccgcattcgc
    16681 cagaccggag ccacggagtt tggcctctgt cggccagatt tcagggacat acactgccga
    16741 ggcatagcaa acgtacatat agacgaaagt aatcaggaag aaaccaatca acgttatgag
    16801 caacatactg gtttgcagtg aatagatata tccgagcacc gcaatcagga ttaatagccc
    16861 cacacccatt gttttacgcg gaattttatc catcaccagc atggcaataa aaataccaaa
    16921 tggcgcgcca aacatactca tggtatttaa aacaatcgag tcttttaaat taatcccctg
    16981 ggtcatgaat attgttggca accagttaat tagtgtgtac tgcacaacgt tcatggcaat
    17041 cagcacacaa gagcctaata tcacgcgttt cagtaatact cctgtcagta aggctgaata
    17101 cggcaccgct tgtggcgctt taccgtcatc agcaataacc acaggcggca aaggtttacc
    17161 cgtctggcgt atgacgcctt cttctatact ccgcatgact ttctcggctt cctgataccg
    17221 tccgcgcgat tccagccagc gcggggattc aggaaagtag cgccaggcca gcgccgtagc
    17281 gataagcgac aatattgcag ggataagcag ttgtacccgc cagttccact ctgcactaat
    17341 cagcggcgtg agtcccatcg ctatcaatga acagagcgga tatgaccagt tgccaataaa
    17401 ggaaacccga cttgaccacg ttccacgatt tctaccgggc atatattcgg tgaaaccagc
    17461 aaacagcgta accagtaaag ctcccagccc aactcccatc acaaaacggc aggcaataag
    17521 gaaatccata ttcggtgaga aagcaccgac aaccatcgag gcaatatgaa tggcctcata
    17581 gaggatgaac gcatttctgc gaccggtttt gtcgccaatg atgccgccca ccagcgcacc
    17641 gagaaacatt ccggcggtcg tgattgctga gaatgtggct gtggtggaat tatctgtcca
    17701 gcccaacgct ttcagctgcg cgaggatcaa gccaccaacg gcattactcc agcagacaag
    17761 caagccaaac gcgacgatgg caaacattga cgaatgccag cggcaatccg gtaagcgatc
    17821 cagccgtgca ccacaatgcg gttttgttat ctgttccatg cgataacgtt ccttcaggag
    17881 agtggttatt catcaaagtc gtaagtcatg atcactttga ttgcggtttt atcgaccatc
    17941 gcatcaaacc cttcgcgcca ttgcgacagg ccgatacgat gcgtgatcat cggtttgact
    18001 ttgatagcgc cgctggctaa tagccggata gcgttacgcc atgaggtgga gtcataggcc
    18061 atatgcccaa tgatgctttt attccaggcg gtaatgtcat taatcgagaa atcaagaggt
    18121 ttgaagccca ttccaacgcg taccacttcc ccattcgggc ggagcatttc gatggcttgt
    18181 ttcagtgcga tattggcacc ggagcattca atcaccagtc ccagattatc tttgccacaa
    18241 atttgctggc agcgcgccac cacatcttcg gtagaaccat ttactactgc cgtcgccccc
    18301 agttcttttg caaccgggaa gcggaccgcc acatcttctt gcagaccaac gacgacgata
    18361 tttaccgccc ccataatccg cgccatttgt acggaaaaca gcccgagtgg gccagtgccg
    18421 atgacgacaa catcctgacc aggaaggaat ttcgattgct gcgcgatgga tttataggca
    18481 ttacagatag ggtcaagtac ggctgcgtcc tcataatcaa caccatcagg gatttcccac
    18541 aacgcatgac gatgaatttt gagaatttca ccaggaacca gacaatattt ggaaaaacca
    18601 ccgccccagg tattgttatc cagaccaagg tttacttttt ctgtacaaca cagaaaatca
    18661 ccctgttcac aggccggaca aacgccgcaa acgtgaccgc tgttatccga cacgacgcgt
    18721 tgccccactt tccagtcttt gactttttca ccaacctgcg caatacaacc tgcgaactca
    18781 tggccgcgga tagagttaaa ctcatcagaa ccgctatcga cattgtagtg cttcatgtct
    18841 gcgccgcaga ttgccgcggc tttaatttca atcactacgt cttccgggcc acaaataggt
    18901 tgtgggacat caatcatctt gtagccgcca aaggccttgc caaaccgagc cagtgctttc
    18961 attgcgtcat cctctgtgtt ttgttttgct tagatggcca tcatacttac ccacatgatt
    19021 tttgaggtca acattaaatc tgtaaaacac agataatgat ctgcgtttta caacttagat
    19081 cacaattacg cagaaaatgg ctgggtctgt tacaggttgg tggaaggcgg gacgcaaaaa
    19141 gagcaaattc gagagggatt accggccaaa taggtctgag acttaagttc aggagaagtc
    19201 attcaggcgt gaacgcgact gcaagagatc ggactgggac ttagccaggt cgggtcgaga
    19261 taattgagac gagtcgtggg gtcattgttg gctatcccct tcatagccgc ggcaaaaagg
    19321 ggatgaacca cttacttaaa catagcaata ggctttgctg agataccaaa atcttctttt
    19381 aactgctgtt tgctcttcat gaccatctga ccatttgtgt caattgtcat atgctgtgct
    19441 tcggtattat ggcgagcttg ccacagcatc accagttgca ggcagttcgc tttttgttcc
    19501 tcggtcaacg caacgccatc gggccatttc cccagttcaa cggcggtcga caaacgctgg
    19561 tatacctcag gcgtcatgct gttgataatg tcatcaagat tcataatttt tccgctcctg
    19621 tggaataagt tgctgaatcg ttttttcaac cgttgatttc ttcgccgttt tcgccatcgg
    19681 taaagcgtaa agaggcagag ttaacacaat aacgttcgcc cgttggctgc ggcccgtcag
    19741 ggaagacatg ccccagatgg gcatcacagt taccgcaacg aatttctatg cgctgcattc
    19801 catgtgacaa gtctttgata taacgaatgg attcttcact caccggttcg tagaaactgg
    19861 gccaaccaca gccggaatca tacttggttt gggaatgaaa cagcggggca tcgcagatca
    19921 aacagtgata tacgccgtca cgcttgttat gcagtaaacg acccgtaaat ggcggctctg
    19981 tcccatgatt ctgcgtcacg taaaactgca tctcggacaa attttttttc agttcttctg
    20041 ccgaaggttt attagccatt tgctcacatc tcactttaat cgtgctcaca ttacgtgact
    20101 gattctaaca aaacattaac accaactggc aaaattttgt cctaaacttg atctcgacga
    20161 aatggctgca cctaaatcgt gatgaaaatc acatttttat cgtaattgcc ctttaaaatt
    20221 cggggcgccg accccatgtg gtctcaagcc caaaggaaga gtgaggcgag tcagtcgcgt
    20281 aatgcttagg cacaggattg atttgtcgca atgattgaca cgattccgct tgacgctgcg
    20341 taaggttttt gtaattttac aggcaacctt ttattcacta acaaatagct ggtggaatat
    20401 atgactatca aagtaggtat caacggtttt ggccgtatcg gtcgcattgt tttccgtgct
    20461 gctcagaaac gttctgacat cgagatcgtt gcaatcaacg acctgttaga cgctgattac
    20521 atggcataca tgctgaaata tgactccact cacggccgtt tcgacggtac cgttgaagtg
    20581 aaagacggtc atctgatcgt taacggtaaa aaaatccgtg ttaccgctga acgtgatccg
    20641 gctaacctga aatgggacga agttggtgtt gacgttgtcg ctgaagcaac tggtctgttc
    20701 ctgactgacg aaactgctcg taaacacatc accgctggtg cgaagaaagt ggttatgact
    20761 ggtccgtcta aagacaacac tccgatgttc gttaaaggcg ctaacttcga caaatatgct
    20821 ggccaggaca tcgtttccaa cgcttcctgc accaccaact gcctggctcc gctggctaaa
    20881 gttatcaacg ataacttcgg catcatcgaa ggtctgatga ccaccgttca cgctactacc
    20941 gctactcaga aaaccgttga tggcccgtct cacaaagact ggcgcggcgg ccgcggcgct
    21001 tcccagaaca tcatcccgtc ctctaccggt gctgctaaag ctgtaggtaa agtactgcca
    21061 gaactgaatg gcaaactgac tggtatggcg ttccgcgttc cgaccccgaa cgtatccgta
    21121 gttgacctga ccgttcgtct ggaaaaagct gcaacttacg agcagatcaa agctgccgtt
    21181 aaagctgctg ctgaaggcga aatgaaaggc gttctgggct acaccgaaga tgacgtagta
    21241 tctaccgatt tcaacggcga agtttgcact tccgtgttcg atgctaaagc tggtatcgct
    21301 ctgaacgaca acttcgtgaa actggtatcc tggtacgaca acgaaaccgg ttactccaac
    21361 aaagttctgg acctgatcgc tcacatctcc aaataagttg agatgacact gtgatctaaa
    21421 aagagcgact tcggtcgctc ttttttttac ctgataaaat gaagttaaag gactgcgtca
    21481 tgattaagaa aatttttgcc cttccggtca tcgaacaaat ctcccctgtc ctctcccgtc
    21541 gtaaactgga tgaactggac ctcattgtgg tcgatcatcc ccaggtaaaa gcctcttttg
    21601 cattacaggg cgcacacctt ctctcgtgga aacctgcggg tgaagaagaa gttctgtggt
    21661 tgagcaacaa cacaccgttc aaaaatggcg tcgctattcg cggtggcgta ccggtttgct
    21721 ggccgtggtt tggtccggcg gcacaacaag gtctgcctgc gcacggtttt gcccgcaacc
    21781 tgccgtggac gctgaaatca catcgtgaag atgctgatgg cgtagcgctg acttttgaat
    21841 tgacacaaag cgaagagacg aaaaaattct ggccgcacga ctttacgctg ttagcgcatt
    21901 tccgcgtggg taaaacctgt gaaatcgatc ttgaatcaca tggcgaattt gaaaccacct
    21961 ctgccctgca tacctacttt aacgtgggtg atatcgctaa ggtaagcgtc agtgggctgg
    22021 gcgatcgctt cattgataaa gtaaatgacg cgaaagaaga tgtactgacc gacggtattc
    22081 agaccttccc tgaccgtacc gatcgcgtgt atctgaatcc gcaagattgc agcgtgatta
    22141 atgatgaagc gctgaatcgt attatcgccg taggccacca gcatcatctg aacgttgtcg
    22201 gctggaaccc gggaccggcg ctttcagtta gcatgggcga catgccggat gatggctaca
    22261 aaacatttgt ttgtgtagaa acggcttacg cttcagaaac gcaaaaagtg accaaagaga
    22321 aacctgcaca tctggcgcaa tccattcgcg ttgcgaaacg ttaatttgcg ttaatgttgt
    22381 gtgccgggtg cgatgcatcc ggcacgtatc aacatcacac catatccagc gcagtttttc
    22441 cttttggtgc cggatacgct ttatccagca tggttaattc cgctgacgaa agttcgacct
    22501 caagcacagc cgcattctgt tggacatggg caatcgtggc cgcttttgga atcgccatca
    22561 caccctgatg gctgattacc cacgccaaca atacttgtgc cgcgctgata ttgtgagcat
    22621 gtgcaatttc gttgactacc gcgtttttta acagtccatt gcgcaaccgc ccggcctggg
    22681 ctaatggact gtaagccatc accggcatct gctgttgctg gcaccagggg agtagatcgt
    22741 actcaatccc tcgtgaaccg agatggtaaa gcacctgatt agtggcacac tgatttcccc
    22801 ccggcagctg ccagagttcc tgcatatcag catgatcaag gttagaaacg ccccagcggc
    22861 ggatttttcc ctgggcgatc aatttttcca tcgctgccac agtctcttca aaagcgaaac
    22921 tgccagacca gtgtaataag tagagatcga gataatcagt attgagacgg cgtaaactgg
    22981 cttcgcatgc atttatcgct ttttgcccgc cagcattcca cggatagact ttagagacga
    23041 gaaagacgtt atcgcgcaga ccggttaatg cttccccaac caccttttcg gcaccgccat
    23101 cggcatacat ttcggcggta tcaatgaggg ttaaaccgag ttcaatgccc gcgcgtagtg
    23161 cagcaacttc tgttttgcgc tgactggcat cttcgccaat ataccatgtt ccctgcccta
    23221 tggctggcag tgagacatcg ccactaaatt gaatcatttt ttgttgcatt gtttcctccc
    23281 aggtattgca ccaccgtaat gcaaaacagg gcgtgacgcc ctgtttttat gcacaaaatg
    23341 ccctggaaag atgcattatc agaatttgta ggtgatcccg gtagaaatca ggccagtcca
    23401 ggatttatcc accatcgggc tgtcagtaac ttcatcagac agacgggtat agcgcgcgga
    23461 accgtaaaca ctccagtcgc cgaggaagtt gtagctggcg ctcagctcca ggtaagggct
    23521 ccagctgtcg ttcgggttat agccacgcag accgctgcga gcggactctt tgcgcgatac
    23581 gccataatag tattcgttct ggttttcgct gttccactgc acaccaatac ccggagtcac
    23641 ggtcaggcca ccgttggtgt aacgatacaa ccaggccata tcccagacaa tgccgttgct
    23701 gttatccagg gtatcgccag ccagggtggt acgcaggtaa ccgtactggg taaagtgagc
    23761 ataagacaga ccagccatca tggtgctctt acggtcatcc aggtgacgca tttggtgatc
    23821 gccactgtct ttcgctttga agtaaagcgg cgaccagtaa gcggtaattg aaagtttatc
    23881 cgttgcgtca ttccacaggt agtaaccacc acctaagcca cggaaccaga agttatcgcc
    23941 ttcatagttg attaccggta ctgggtaaac atcggtatcg taatctttat atgggtgctc
    24001 aacgacacct acgcctgcgc ccagggaaaa tttaccttca gcgtgcgcta cgcctgcaga
    24061 cgttgcgata agcactccaa gtgccagaag tttgagtttg gtcacaatta atcattcctt
    24121 aaacaaatgt ttagcgggcg acaaagttta cccgtcaata catcgaaacc caaccttttt
    24181 acgttttcat tttttaaagt aactgtttaa ttttcctgac gcggatgaca ccgcgcttac
    24241 agccaaatga atttagcgtt actggctacc ctggtcttta cattaattat gcaaaattta
    24301 tggatgagtt gttgatatgc cattgaaatt aagaaagccg tgcaggcaag ttttccattt
    24361 gccatctacg cttaattttg aaggtgtatc accgggcacg ttgttctcat cgtcgataaa
    24421 atggcatgag agttgctgtg ttttagcaag agacgtcgtt cagtttacct cttccgggag
    24481 cctctactat tcatatgaac ggctcttaac ctgtgctaaa aaacgaaagg acggcatacc
    24541 atgaatatat tcgatcacta tcgccagcga tatgaagctg ccaaggacga agagttcaca
    24601 ctgcaggagt ttcttaccac ttgtcggcaa gatcgcagtg cttatgccaa cgcggctgag
    24661 cggctattga tggctatcgg tgagcctgtc atggtcgata cagcccagga acccagactt
    24721 tctcgactct tttctaaccg ggtcattgca cgttatccgg catttgaaga gttttacggc
    24781 atggaagacg cgattgaaca gattgtctct tatctgaaac acgcggctca ggggctggaa
    24841 gagaagaaac aaatcctgta tctgctgggg cctgtgggtg ggggtaaatc atcgcttgct
    24901 gagcgactga aatcattaat gcagctcgta ccgatttatg tattgagcgc caacggtgag
    24961 cgtagcccgg tcaacgatca tccgttctgt cttttcaatc cgcaggaaga tgctcagatt
    25021 ctggaaaaag agtatggcat tcctcgccgt tatctcggca ccatcatgtc gccatgggcg
    25081 gcaaaacgcc tgcatgaatt tggtggcgat atcactaagt tccgggtagt gaaggtctgg
    25141 ccgtcaattc tgcaacaaat tgctatcgcc aaaacggaac ccggtgatga gaacaaccag
    25201 gacatctccg cgctggttgg gaaagtcgat attcgtaaac tcgaacacta cgcgcagaat
    25261 gacccggacg cctacggcta ttccggtgcg ctgtgccgcg ccaaccaagg gatcatggaa
    25321 tttgtcgaga tgtttaaagc accgataaaa gtgctgcatc cgctgttaac cgccactcag
    25381 gaaggtaact acaacgggac ggaaggtatc tccgccctgc cgttcaacgg gattattctc
    25441 gcccactcga acgaatccga atgggtcact ttccgtaata acaaaaacaa cgaagccttc
    25501 ctcgaccgtg tttacatcgt gaaggtgccg tattgcttac gcatttccga agagatcaaa
    25561 atctacgaga aattgcttaa tcacagtgaa ttgactcacg ccccctgcgc ccctggcacg
    25621 ctggaaacgc tgtcacgttt ttccattctt tcgcgcctta aagagccaga aaactccagc
    25681 atttattcaa agatgcgggt ttatgatggc gaaagtctga aagacaccga tcccaaagcc
    25741 aagtcgtatc aggaatatcg tgactacgcc ggtgtcgatg aagggatgaa cggtctgtcg
    25801 acgcgttttg cgtttaagat cctctcccgc gtgttcaact tcgatcatgt agaagtggcg
    25861 gcaaacccgg tccatctgtt ctacgtcctg gaacagcaga tcgagcgcga gcagttccca
    25921 caagagcagg cagaacgcta tctggagttc ctgaaaggtt atctgatccc gaaatatgcc
    25981 gagtttatcg gcaaagagat ccagacggcc taccttgaat cctattccga atatgggcaa
    26041 aacattttcg accgttatgt tacctacgcg gatttctgga ttcaggatca ggagtatcgc
    26101 gatccggata ccgggcagct gtttgaccgc gagtctctta acgccgagct ggagaaaatc
    26161 gagaaaccgg cggggatcag taatccaaaa gatttccgca acgagattgt taacttcgta
    26221 ctgcgcgcca gagcgaataa cagcggacgc aatccgaact ggaccagcta tgaaaaactg
    26281 cgcacggtta tcgagaagaa aatgttctcc aataccgagg agctgttgcc ggttatctcg
    26341 tttaacgcca aaacgtcaac cgacgagcag aagaaacacg acgactttgt cgaccgtatg
    26401 atggaaaaag gctacacccg taaacaggtg cgtttactgt gcgaatggta tttgcgcgta
    26461 cgtaaatcgt cttaacaacc ctggcccggt acacatgtta ccgggcctac aacgacagcg
    26521 aaccgtgggc ctgagaagcg gcaacacagg cgtagcatac agttggcaaa tgtagtacgg
    26581 ggggcatatg acctggttta ttgaccggcg tctgaacggc aaaaacaaaa gcatggtgaa
    26641 tcgccagcgt tttttacgcc gttataaagc gcaaattaaa cagtcgatct ccgaggccat
    26701 taataagcgt tcggtgactg acgtcgacag cggcgaatcc gtatccattc ccacggaaga
    26761 tattagcgaa ccgatgtttc atcaggggcg tggcggtctg cgccaccgcg tgcatccggg
    26821 caatgaccat ttcgtccaga acgaccgaat tgaacgtccc cagggtggcg gcggaggttc
    26881 cggtagtggt cagggccagg ccagccagga tggtgaaggt caggatgaat ttgtctttca
    26941 gatttcgaaa gatgagtatc ttgatttgct ctttgaagat ttggccttac cgaatctgaa
    27001 acaaaaccaa caacgccagc tgaccgaata taaaacgcat cgggcgggtt ataccgctaa
    27061 cggcgttccg gcaaatatca gcgttgtgcg ctccttgcag aactcactgg cgcgacgcac
    27121 agccatgacg gcaggcaagc ggcgggaact tcatgcactg gaagagaatt tggccatcat
    27181 cagcaacagt gaacctgcgc agctgctgga agaggaacgt ctgcgcaaag aaattgcaga
    27241 attacgtgcc aaaattgaac gcgtcccttt tattgatacc ttcgatttac gttacaagaa
    27301 ctacgagaag cggcccgatc cctccagcca ggcagtgatg ttttgcctga tggacgtttc
    27361 cggttcaatg gatcaatcca ctaaagatat ggctaagcgt ttttatattc tgctgtatct
    27421 gttcctcagc agaacgtata agaacgtgga agtcgtatac atccgccatc atacccaggc
    27481 gaaagaagtc gatgaacatg agtttttcta ctcgcaggaa acaggcggca ccattgtttc
    27541 cagcgccctg aaactgatgg atgaggtagt gaaagagcgt tataacccgg cacagtggaa
    27601 tatttacgct gcacaagcat cggacggcga taactgggcc gatgactctc cgctttgcca
    27661 tgaaatcctg gcgaaaaaat tattacctgt tgttcgttat tacagctata tcgaaattac
    27721 ccgtcgtgca catcagacat tgtggcgaga atatgagcat ctgcaatcta ctttcgacaa
    27781 ctttgcgatg cagcacatcc gcgaccagga tgatatttat ccggtgttcc gtgaactgtt
    27841 tcataaacaa aattcaacag ctaaagacta aaactatcag ccaggtcatt atcgcctggc
    27901 tgatttttag cttactgtaa attatctcat ttattacata cattagctta caatcgcttt
    27961 aaatatgaca gcataacctt tacataattt agttccagaa aacaatcatt cggaaaaatg
    28021 attcagtcaa cacgtatttc catggggtta ttctttaaat attttttatc gttaacgaaa
    28081 attgatcctg gtcaaaacta tatatctctg ccatcaataa aatccagcac tcacattgct
    28141 ctccttttta tggtttctat gggtacacaa aaattaaaag ctcaaagctt ttttattttc
    28201 agtttattgc tgacgt
//
LOCUS       DAJOWB010000053        27774 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJOWB010000053 DAJOWB010000000
VERSION     DAJOWB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.053"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1865
                     /gene="dhaR"
                     /locus_tag="OHO85_002466"
     CDS             <1..1865
                     /gene="dhaR"
                     /locus_tag="OHO85_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCQ2671827.1"
                     /translation="WERCNKLMKRETWNVPHQAQGVTFASIYRRKKAMLTLGQAALED
                     AWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGFNDGTYCAEGIIGTCALSLA
                     AISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGTIALACPVEQTTAADLPLTL
                     AIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVISWDEQGNLQFINAQAARVLR
                     LDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFESQHQFIDAVITLKPIIETQ
                     GTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQTRRLIHFGRQAARSSFPVLL
                     CGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAALAEEFIGGDRTDNENGRLSR
                     LELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDARRLIPIDVKVIATTTADLA
                     MLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNNKLRSLEKRFSTRLKIDDDA
                     LARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPEHLFTEQATDDVSATRLSTS
                     LSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRKMKQHGIDAGQFKRRV"
     gene            complement(1965..4850)
                     /locus_tag="OHO85_002467"
     CDS             complement(1965..4850)
                     /locus_tag="OHO85_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pertactin family autotransporter"
                     /protein_id="HCQ2671828.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTSGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVASGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHSGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTNNSNLDTLALSHSTVDFAS
                     HASTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPTPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVR
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTEYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYHFSF"
     gene            complement(5694..6785)
                     /gene="ychF"
                     /locus_tag="OHO85_002468"
     CDS             complement(5694..6785)
                     /gene="ychF"
                     /locus_tag="OHO85_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCQ2671829.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVLPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(6902..7486)
                     /gene="pth"
                     /locus_tag="OHO85_002469"
     CDS             complement(6902..7486)
                     /gene="pth"
                     /locus_tag="OHO85_002469"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCQ2671830.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEGVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            7764..8042
                     /gene="ychH"
                     /locus_tag="OHO85_002470"
     CDS             7764..8042
                     /gene="ychH"
                     /locus_tag="OHO85_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCQ2671831.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(8097..9776)
                     /gene="dauA"
                     /locus_tag="OHO85_002471"
     CDS             complement(8097..9776)
                     /gene="dauA"
                     /locus_tag="OHO85_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCQ2671832.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(9901..10848)
                     /gene="prs"
                     /locus_tag="OHO85_002472"
     CDS             complement(9901..10848)
                     /gene="prs"
                     /locus_tag="OHO85_002472"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCQ2671833.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(10999..11850)
                     /gene="ispE"
                     /locus_tag="OHO85_002473"
     CDS             complement(10999..11850)
                     /gene="ispE"
                     /locus_tag="OHO85_002473"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCQ2671834.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGADISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(11850..12473)
                     /gene="lolB"
                     /locus_tag="OHO85_002474"
     CDS             complement(11850..12473)
                     /gene="lolB"
                     /locus_tag="OHO85_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCQ2671835.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLNEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            12687..13943
                     /gene="hemA"
                     /locus_tag="OHO85_002475"
     CDS             12687..13943
                     /gene="hemA"
                     /locus_tag="OHO85_002475"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCQ2671836.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRGQAEQVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            13985..15067
                     /gene="prfA"
                     /locus_tag="OHO85_002476"
     CDS             13985..15067
                     /gene="prfA"
                     /locus_tag="OHO85_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCQ2671837.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            15067..15900
                     /gene="prmC"
                     /locus_tag="OHO85_002477"
     CDS             15067..15900
                     /gene="prmC"
                     /locus_tag="OHO85_002477"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCQ2671838.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGKGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALARERPDCEITAVDRMPDAVALAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            15897..16289
                     /locus_tag="OHO85_002478"
     CDS             15897..16289
                     /locus_tag="OHO85_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCQ2671839.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTMLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            16293..17102
                     /locus_tag="OHO85_002479"
     CDS             16293..17102
                     /locus_tag="OHO85_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCQ2671840.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            17138..17992
                     /gene="kdsA"
                     /locus_tag="OHO85_002480"
     CDS             17138..17992
                     /gene="kdsA"
                     /locus_tag="OHO85_002480"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCQ2671841.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(18140..18247)
                     /locus_tag="OHO85_002481"
     CDS             complement(18140..18247)
                     /locus_tag="OHO85_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCQ2671842.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(18676..18783)
                     /locus_tag="OHO85_002482"
     CDS             complement(18676..18783)
                     /locus_tag="OHO85_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrB"
                     /protein_id="HCQ2671843.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(19188..20288)
                     /gene="chaA"
                     /locus_tag="OHO85_002483"
     CDS             complement(19188..20288)
                     /gene="chaA"
                     /locus_tag="OHO85_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCQ2671844.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            20558..20728
                     /gene="chaB"
                     /locus_tag="OHO85_002484"
     CDS             20558..20728
                     /gene="chaB"
                     /locus_tag="OHO85_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCQ2671845.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVA"
     gene            20946..21641
                     /gene="chaC"
                     /locus_tag="OHO85_002485"
     CDS             20946..21641
                     /gene="chaC"
                     /locus_tag="OHO85_002485"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCQ2671846.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21685..22038)
                     /gene="ychN"
                     /locus_tag="OHO85_002486"
     CDS             complement(21685..22038)
                     /gene="ychN"
                     /locus_tag="OHO85_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCQ2671847.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            22223..23617
                     /gene="ychO"
                     /locus_tag="OHO85_002487"
     CDS             22223..23617
                     /gene="ychO"
                     /locus_tag="OHO85_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCQ2671848.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASIDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(23618..24268)
                     /gene="narL"
                     /locus_tag="OHO85_002488"
     CDS             complement(23618..24268)
                     /gene="narL"
                     /locus_tag="OHO85_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCQ2671849.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(24261..26057)
                     /gene="narX"
                     /locus_tag="OHO85_002489"
     CDS             complement(24261..26057)
                     /gene="narX"
                     /locus_tag="OHO85_002489"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCQ2671850.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            26396..>27774
                     /gene="narK"
                     /locus_tag="OHO85_002490"
     CDS             26396..>27774
                     /gene="narK"
                     /locus_tag="OHO85_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCQ2671851.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGR"
BASE COUNT         6822 a         6889 c         7125 g         6938 t
ORIGIN      
        1 cctgggagcg atgcaataag ctgatgaaac gggagacatg gaacgtacca catcaggccc
       61 agggcgtgac atttgcttct atttatcggc gtaagaaagc gatgctgacg ctcgggcagg
      121 ctgcgctgga agatgcctgg gaatatatgg caccgcgaga gtgtgcgctg tttatcctcg
      181 atgaaaccgc ctgcattctc agccgtaatg gcgatccgca aaccttgcag cagctaagtg
      241 cactgggatt caatgacggc acgtattgcg ccgagggaat tattggtact tgtgcgctat
      301 cgttagcggc tatctctggt caggccgtga aaacgatggc cgatcaacat ttcaaacagg
      361 cactctggaa ctgggccttt tgtgcaacgc cgttgtttga cagcaagggc cgattgacgg
      421 gaacaatagc gctggcgtgt ccggttgagc aaactaccgc agctgatttg ccgttgacgt
      481 tggcaatcgc ccgcgaggtc ggtaatttac tgctgacgga cagtttgctc gctgaaacta
      541 accgtcattt aaatcaactt aatgccctgt tagaaagtat ggatgatggc gtgattagct
      601 gggacgagca gggtaatttg caatttatta atgcccaggc ggcgcgggtc ttgcgccttg
      661 acgcgacggc aagtcaggga cgggcaatca ctgaactctt aacgttaccc gccgtattgc
      721 aacaagcaat aaaacaggca catccgctca aacacgtaga agcaaccttt gaaagtcagc
      781 accagtttat tgatgcggtg ataaccctta aaccgataat agaaacgcag ggaaccagtt
      841 ttattttgtt gctccatcct gtggaacaga tgcggcagtt gatgaccagt caattaggaa
      901 aagtcagcca taccttcgct catatgccac aggacgatcc gcaaacccgc cgcttgattc
      961 attttggtcg ccaggcggcg cgcagtagct ttcctgtcct gctttgtgga gaagagggcg
     1021 tgggcaaggc actgctaagt caggcaattc ataatgaaag tgagcgtgct gcaggtcctt
     1081 atatcgccgt caattgtgag ttatatggtg atgcggcact ggcggaagaa tttattggtg
     1141 gcgatcgcac ggacaatgaa aatggccgtc tgagtcggct ggaactggcg cacggcggca
     1201 cgctgtttct tgaaaagatt gaatatctgg cggtggagtt acagtctgct ttgcttcagg
     1261 ttatcaagca gggtgttatc acgcgactgg atgcgcggcg tttaatacca attgatgtca
     1321 aagtgattgc cacaacgacc gcggacctcg caatgctggt ggaacaaaat cgttttagtc
     1381 gccagctgta ttacgcgctg catgcatttg aaattaccat cccgcctctg cgtatgcggc
     1441 gtggcagcat tccggctctg gtgaataaca aattacgcag tcttgaaaaa cgcttctcta
     1501 cgcggctgaa aattgatgac gatgccctcg ctcgcctggt ttcctgtgca tggcctggca
     1561 acgattttga actttacagc gtcatcgaga atcttgctct gagtagtgat aatgggcgta
     1621 ttcgcgtcag tgatttgccg gaacatctgt ttaccgagca ggcaacagat gatgtcagcg
     1681 ccacacgcct ttccaccagt ctgtcatttg cggaagttga aaaagaggca attattaacg
     1741 cagcccaggt cacaggcggt cgcattcagg aaatgtcggc tttacttgga atcggccgca
     1801 ctacgctgtg gcggaaaatg aagcaacatg gcattgatgc agggcagttt aagcgccggg
     1861 tatgaaagac agagacgatt tctgatacat cagagtgatc tatatttcat tccggcgcac
     1921 gctaacattt ttcagcatcg tttaagggct tgtctacccc gtacttaaaa gctgaagtga
     1981 tatcctccgt tgacttgctt ctgatcaaag aggttaccct gcgtgtaatc cgcttcgagg
     2041 tagaatgtgt gctgtttgtt atactgtgca ctgactccca cgccgttatt ccagccatta
     2101 cctttgaaac tgtacttctc ccgggagttg ttcaacagat attctgtatc ccctgaaaac
     2161 tcgcggatag cgccagtctt cacatacata ttcagctggc tgttgcctgc ggtgatgtca
     2221 tatcccagta tcatgctggc acgccccagc agcgattcgt agtgattcag atgtatattg
     2281 agaccattac tcgccttcat agccatctca ttctggtggc tgtatgtgag ctgggtttgc
     2341 ggctctatat agaacccata accagtaggg gtcaggttga acctctgccc ggcttccagg
     2401 gagatgctca atccattcgc agtgccgttg gcgttaacgc cgttgttctg actgtcccgt
     2461 acgtggaaac tatttttctg gcgcgatgct tttacaacga gatcgctgta aaaaccgttg
     2521 tgtgccatgt aactggcgta cattcccatg tagtctgaac gtgcggtacc gtcgccaccg
     2581 ctatagtccg gcgatgcatg tgttgagcca atatacagac cgacatacaa cggcattaca
     2641 tcagagagac gtttatcccc accaaactgg ataccgctgt aacccatgtc aaagccgctc
     2701 agtttgccac tggcgaagga gtccaggctt cccccataac tgcgcagcca gatattaccg
     2761 tctttactct gatttcgcag gtcacccatt cgttgcatca gcgtacggtt ttcaacatag
     2821 tttaataagt aaccgacatt gagataattg ccgccagcat ctgcggtcgt ggtgggttta
     2881 ggtgtgggag tgggggtagg cgtaggatcg ggttcaggcg taggatcggg gttgactatg
     2941 ggaggctgag cgggagccgg tgttggttca ggattaggag tcggttccgg aactgtcccg
     3001 gaagcgtaaa gctcccagtt agtgccattt ttacgcacat catacagata tccccccaac
     3061 tcaacctgag aagacgcgct gaacgaggcc gcgccgtcag tggtttttac cactgtcaga
     3121 acttcatttc ccgttgtggc ttcgctgccc tggttacgga tagccaatac atgattacca
     3181 gcactactcc cgctgatatt caataaatcc cctttattat taacgccatt accctcacca
     3241 acaacatcag cacgcataat aaaggtactg ttaccgctca ggttctctac gtttaatgtg
     3301 gtaaatgtgc cggcagttga tgcgtggctg gcaaaatcga cagttgaatg gctcagcgcc
     3361 agcgtgtcga gattagaatt atttgttacg ttccagacgc tgttattcat tgcaacgtcc
     3421 agtttcccgc cattgacatt atcgctgagg gaggaacctg tccaaaccga cccagagtgc
     3481 atatccagat tgattagccc acctttggaa agaacgctac cgttgataag catacgaccc
     3541 gaggcgttga tgcggctggc ggcataacca tcgtcatgtt gcgttgcgat ggccatctgg
     3601 tcgggtgtac tcatatcaat gaccagatcg ctcgtgaggt cgatctggct gttggtcatg
     3661 gcataaatcc ccctggctcc tgccgcgccg gtgatagcca aactgtctcc ggttattcta
     3721 ccgccgctga gcgcccacaa acccagcgcc agactgccat tacgatcaac cgtaatatcg
     3781 gtattttgca tgttgataac agccgttgcc gtctgggccg aggcaccata agaaccgccg
     3841 gaaaagatgc tgtttcgttg tgctgccgtg ccagaaaaat tgattgtcgc gtctgaacca
     3901 ctggctacga ggccaccgcc ctgcgcggaa gaaatatggc tatctgcacc gattgtggtt
     3961 gtaccaccac gaacttcgac gccattggcc gcggctccag ttacatcaac agtgagtgca
     4021 ttcgcgctaa cctggccaaa gctccagagg ccgtgtgcat tatcgccact ggttttaatg
     4081 gaactgtttg ttccgagatc gacaacagag tttttctgta cgtttatccc catggcgctg
     4141 tagccctgaa catcgattgt caggtctgtc gccgtaaaac gcgcagcacc attggcgtta
     4201 ttgccgttga gaccaccgat ataaacacct gtacttccat cggtcttgat tttacttccg
     4261 cttccaagat cgacactggt gccatagtca ttgatggtta ggcctatacc gttcgagttt
     4321 tcaatggtga attgagtggc tgttagtgtt gagctatgcc caataatgat gccgtcatcg
     4381 ttactcttaa tggtgctgcc tgtgcctaag tcagcatggg tatagtcacc aattaaatta
     4441 atgccgatgg cagaggtttg accaacaaca tctactgtga gtcggttagc tgtcagggag
     4501 ctgtttttgc cctgaataat gattcctttt gcatatccag aggcgtcgtt gacattgacg
     4561 gtgacatcat tacccaggtt gatattgcca ggcgtgtttc ctgctggggt cattacacca
     4621 tagaggtttc ctgatggatc ggcggtatct cctgtaattc tgtcaccatc attaatagta
     4681 atattggtgc catattgact tcctgtatca gcagctatca gcagggggga aaagataatt
     4741 gcattcaaac caatagcgcc aaattttatc agttgaattg agggcgaacg gatttttaat
     4801 tcagcgagac gatcggcttt ggtattccca ttgtgttgtt tgatgcccat accactccta
     4861 tatagtaccc aggctatggg tctatggatt ttgtcatttt gtttaagtca atgaataata
     4921 tcctcttatc atcatgatat ttgtatggtg caatcatgat gagcttctct gagaataatt
     4981 ctctattacc gggcaaaatc agaaacacag gaattaatat atatttaaca tcccccttac
     5041 aaaggtgagt ttagatcgag ttattcccta agttgtcgtt acttactcca attcgttact
     5101 atgaatatga caaaaacatt acaccaaaca gcggttaaat aatttggtga atatttttgg
     5161 taataatcct aattagttat gggattttag cttgatgtat ctgcataaca aaaacaatat
     5221 gcgccatttt tgcattacta ataagaaaaa gcaaaaaaca tcgatattct tttttgaaca
     5281 atggattttc tggcatatcg ttattatcgt gctattggtt atcattttca tcttgccagg
     5341 atcgcacgta atcacgttat tcggggactt tatctgcggg atattaccct tcaggtaagt
     5401 ccttttctcg ccagcatatt ggagcgtgat caatattgat cagctgtgaa gagggctgat
     5461 gtgtagtgga acaaaaactg acgttatatc actgctaatg agatatccgt catctgaaca
     5521 cttatgtctt taattattct ggtggttcac tgtaggcaat aagcacaaaa gtgtaggatg
     5581 ttacaagaat gattaggact cggtgaaatg aaaaatccac gcaattgcgt ggattttata
     5641 tacttttgcg ctcttcatga gatttagcga aacctcatga gacaacaaat taattagacg
     5701 ttgaaaagga agttcatcac atcgccatct ttaacgatgt aatctttacc ttctgcacgc
     5761 attttgccag cttctttcgc gccttgttca cctttgtaag tgatgaaatc ttcaaacgag
     5821 atggtttgtg cacggataaa gcctttttca aaatcagtat ggattttgcc cgctgcctgc
     5881 ggcgcggttg ctccaaccgg aatggtccat gcacgcactt ctttcacccc agcggtgaag
     5941 taagtttgca ggttcagcag cttataaccg gcacggatca cacggttcag gcccggctct
     6001 tccagcccaa gctcctgcat aaactcgtca cgttcttcgt cgtccagttc ggcaatgtct
     6061 gcttcaacag cagcacaaac cggaagcaca acagaacctt ctttcgccgc gatttcacgc
     6121 acctggtcaa gatatgggtt gttttcaaaa ccgtcttcgt tgacgttggc gatgtacatt
     6181 gttggtttca gcgtcaggaa gctcaggtaa cgaatagccg ctttctcttc agcgcttaaa
     6241 tccagcgcgc gcagcatacc tgcgttttcc agctggggca ggcatttttc caggactgcc
     6301 agctcggctt tcgcgtcttt atcgccacct ttggctttct tctgtacgcg atgaatcgca
     6361 cgttcgcagg tgtcgaggtc tgccagcgcc agttcggtgt taataacttc aatatcgtca
     6421 gccgggttaa ctttgccgga aacgtgaatg atgttgtcat tttcaaagca gcgaacaacg
     6481 tgaccgatcg cttcggtttc acggatgttg gtcaggaact ggttacccag accttcgcct
     6541 ttcgatgcgc cttttaccag accggcgata tcgacaaatt ccatggtcgt gggaagcgta
     6601 cgttgcggtt ttacgatttc agccagttga tccaggcgag gatcaggcat tggtacgacg
     6661 cctgtgttcg gctcaatggt gcagaatgga aagttggccg cttcaatacc ggctttggtc
     6721 agcgcgttga acagggtaga tttcccgacg ttgggcaaac cgacgatacc gcatttgaat
     6781 cccatgattt aaatcacctt aatatcttaa taatcaacct gttattgcta acagattgca
     6841 gaaatggaaa taactttgcc tattatacac ggcactcggc aaaaatgccg cagacaacga
     6901 cttattgcgc tttaaaggcg tgcaatcggt tcgttgcttt ggtcaagcca tctgtaaacc
     6961 acatttcagt acaacgcgcc gcttcgtcga tggcttcatc aattaacttc tgttcactaa
     7021 caggcggttt gcctaacaca aaaccgacaa ctttattttt atcgcccgga tgaccgattc
     7081 cgatgcgtaa acggtgaaag ttagggttat tacccaattt actgatgatg tctttcagtc
     7141 cattgtgacc accatggcca ccgcccaact taaatttggc gacgccagga ggcagatcca
     7201 gttcgtcgtg ggccaccaga atttcgtccg gattaatgcg gaaaaaactg gccatcgccg
     7261 caacggcttt gccgctgaga ttcataaatg tagtcgggac taacaggcgg acaccttcgc
     7321 ctccaagggt gactcgcgaa gtataaccaa agaatttagc ctcttcgcgc agcggagcgc
     7381 gcaaacgctc tgccagtaag tcaacgaacc aggcaccagc attatgtcgc gttgcggcgt
     7441 attcagcacc ggggttcgcc aggccgacaa tcaatttaat cgtcacgttt ttttgtcctg
     7501 agtgtgtaca taactggcgc gtagtttact ggttgcggcc ccgcttgaca aaaaactgcg
     7561 tatcaaatgc agataacgta ataattgcct gagtggacta ttagaaagtc agggtgttca
     7621 ggcgtttatt tgtaaagttt tgttgaaata agggttgtaa ttgtgatcac gcccgcacat
     7681 aacccacagg gtgttgtcta tactttacac ataaggaaga ggggtattcc ctgttacaac
     7741 ccagaaagtt ccggaggtga catatgaaac gcaaaaacgc ttcgttactc ggtaacgtgc
     7801 tcatggggtt gggtctggtg gtaatggtgg tcggtgtggg gtattcaatc ctcaaccagt
     7861 taccacagtt taatatgccc cagtatttcg cacatggtgc agtgctaagt attttcgtcg
     7921 gtgccattct ctggctggcg ggtgcccgtg ttggcgggca tgaacaggtg tgcgaccgtt
     7981 actggtgggt tcgccactat gacaaacgtt gccgccgtag cgataatcgc cgtcatagct
     8041 aacaaataat gcagtttgct gaccagtcag tttgcgctga ctggtcaatc tcgtacttat
     8101 aaatccgcca tcgccgcgcg acgattcggg aagaacgcca ggcgtcccgg gatcggttga
     8161 atgccagcgc gagccatagt gcgcagtggc tggaattcca cgttgcacac gcgcagttca
     8221 catccctcgg gcagacgctt cacaaaacgc tggaacgcat caagaccacc agcatcaagt
     8281 accggaacgg catcccactt cagaatcaca atccgtttgc cttcaagacg tgactccagg
     8341 tccgtgaaca agccttcagc agcagcaaaa aacagcgggc caataacacg cagaaccagg
     8401 acatcgtctg gaacatctac gactaccggt gccaggcgag tcatacgtgc gatacgacgc
     8461 ataaacagca gcgatgccag cacgatcccc acgctgatgg caataaccat atcaaacagc
     8521 acggtcaacg acatgcacag cagcatgaca atgatgtcat ctttcggcgc atgacgcagc
     8581 aagtcgacca ctttgtgcgc ttcactcatg ttccacgcca ccatcaacag cagggctgcc
     8641 atggcggaaa gcggcagcca ggagagcagc ggtgccagta ccagcagggc aagaataacc
     8701 agaatagagt ggatcaccgc cgagataggg gaagttgccc cggcacggac gttagcggca
     8761 gaacgcgcga tggcagctgt agcggtaata ccaccaaaga acggagcgat aatattcccc
     8821 agtccctgtc caaccagttc gctgtttgcc ttgtgttttg tcccggtcat accatccagc
     8881 accacggcgc agagcagaga ttcgattgcg ccgagcattg ccattgagaa tgccgcaggc
     8941 agcagtgtgc gaatagaatc ccaggtaagc gtgaattctg aattaggcag atcccacggc
     9001 agcaccagtt gcggcagcag ttgcggaata ccgttaccct gagaaccatc ggccaggacg
     9061 tagtggaatt gcgaaccgat ggtagcaaca tgtccgccga gcaggttaac aatccccatc
     9121 accgcgcaac cagccagcaa ggccggaagg tgaccgggta aacgaatgcc cagacgcggc
     9181 cagaaaacca gaatgccaag cgtcacaatg ccaatggcag catcacccac attaatggtc
     9241 ggcagcgcca taaataatgc gccgactttt tgtagataat gttccgggac atgggccatt
     9301 tgcagaccga gaaaatcttt aatctgcatg gtaccgatgg tgatcccgat acccgaggtg
     9361 aaacctaagg tgacggaaac cggaatatac tcaatcaggc gaccaaagcg tgccagaccc
     9421 atcagaatca aaaagatccc cgacagcaag gtcgcaacca gcagtcctgc cagtccaaac
     9481 tgttgcgaca cgggatagag aattaccaca aatgccgcag tcggaccgga aacgctaaag
     9541 cgtgatccac ccgtcagagc aatgacaatc cccgcaacag ctgcagtata taaaccgtac
     9601 tggggtgcca caccactacc aatagccaac gccatcgcca gcgggatagc aataatcccg
     9661 acggttatcc cggcaatcag gtcacgggta aaccgtgcgg cagtatattt ttctttccag
     9721 caagcgtcga tcagagcgcg gaaaggcatc acatgtgagg aaaatatttt gttcacaata
     9781 atgtttcatc cgtgagcgca tcatctgtca actaaatggc aggtgaagga ggcataggtc
     9841 atacaaatgg atattacaga caaaaaaacc cgccgcagcg ggtctttgag ccgggttcga
     9901 ttagtgttcg aacatggcag agatcgattc ttcgttgctg atacgacgaa tcgcttcggc
     9961 cagcatacct gacagggtca gagtacgcac gttcggcagt gatttgattt catcgctcag
    10021 cggaatggta tcgcagacaa cgacttcatc aattacagag ttacgcaggt tgttcgccgc
    10081 gttgccagag aagatcgggt gagtcgcgta cgcaaataca cgtttagcac cacgttcttt
    10141 cagagcttca gcagctttac acagcgtacc gccagtgtcg atcatatcat cgaccagtac
    10201 gcagtcacga cctgcaacgt caccgatgat atgcatcacc tgggaaacgt tcgcacgcgg
    10261 acgacgtttg tcgatgattg ccatatcggt atcgttcagc agcttagcga tagcgcgggc
    10321 acgcacaacg ccgccgatgt ccggagaaac cacaattggg ttatccagat tcagctgcag
    10381 catgtcttcc agcaggatcg ggctaccaaa tacgttatca accggaacgt cgaagaaacc
    10441 ctgaatctgt tcagcgtgca gatccactgt cagcacacgg tcaacaccga cgctggagag
    10501 gaagtctgca accactttcg cagtgattgg tacacgagcg gaacggacac gacggtcctg
    10561 gcgcgcatag ccaaagtagg ggataacagc ggtgatacga cctgcggaag cacgacgcag
    10621 ggcatcaacc ataacgacta attccatcag gttgtcgtta gtaggggcac aagtggactg
    10681 gatgatgaaa atatcaccac cgcgtacatt ttcattaatt tgtacgctga cttcgccgtc
    10741 gctaaagcga cctacagcgg cgtcgccgag tgaagtgtac aggcggttgg caatacgttg
    10801 tgctagttcc ggggtggcgt taccagcaaa aagcttcata tcaggcacga gaagaacctc
    10861 aggcatgcgt ccattggtgg aaagaatctg ccgaaaactg tgcgggccag gcatgatcct
    10921 ttccaggcgg tgtatttaag agcgcgatgc aacgtctgga acaaggtgac gttgtcaccg
    10981 aaactcagct tgcccggctt aaagcatggc tctgtgcaat ggggaaagat taacgccttt
    11041 cgccacaaag ccattgagcc attccggggc ttgctctagc acctggcggg cttcagactc
    11101 tgtatcaaat tcagcaaaga cacaggcccc tgtcccagtc aggcgcgacg gggcgtattc
    11161 taacagccag gaaagcaccg catcaacctc gcgaaaacgt tttcttgcga taacctcgca
    11221 atcattgctg aattcacatt ttagcaacgt ttctattgac ctttttggcg tattgcgcgg
    11281 gagttcagga tctttaaaaa tcaccggagt cggaatactt acaccagggt gcgccaccag
    11341 ataccacttc tctggcggat ccaccggcgt tagtatttca ccaacgcctt cggcaaacgc
    11401 ggcatgcccc cgaacaaaga caggaacatc tgcgcccagc gtcagcccca tttccgccag
    11461 ctcatccatg cttagcccgc attgccagag atgatttaat gccaccagga ccgtcgcggc
    11521 attggatgaa ccaccgccga gaccgccgcc cattggcaaa cgcttgtcaa tgctgatatc
    11581 cgcaccgctt cccgtcggaa gacgcccgct gtctgccgca gttttcatca acaaccgcgc
    11641 tgcgcgaacg atcaggttat cttcatgttc cacgccttca acgggcgtta acagacgaat
    11701 atccccatcg tcacgaagct caatgctaat ggtgtcgccg taatcaagaa actgaaacag
    11761 cgtttgcagc gtgtggtaac catccgcacg ctgaccggta atgtataaaa acagattaag
    11821 ttttgccgga gagggccact gtgtccgcat tatttcacta tccagttatc catttttaac
    11881 ttgatgcgtt gaccaccgtc ggtgagttcc atattggctg gcatcgcagg ttgcgttttg
    11941 gtgtcataac caccgtaaac aactttccag tttttgccat tctggctgta ggtaatttcg
    12001 ttcaggcggt actggtcgtc cagtttgtag tcggttgcat cacccggtaa acctaaaatc
    12061 cactggcgca agctgttgag cggaatcggc attccggtca atttgccaat catctcttcg
    12121 gcgtcatcgg cggtataacg ctgaccttta ttgtcgacta actgcacgtt acccggttga
    12181 gcattcagct caagttccgt actgcccaat gggttagtca gcagcagacg gtagcgatcc
    12241 tgaccagttt gctgccagaa gaaacgggcg tacacttttt gttggtcaga aatataagcg
    12301 aacgcgccgc gagtttgata ctgattaaga ttgcgcacgt cttgctgatg ctgacgccat
    12361 tgtggcgaat ccgggctttt accaggacct ttgggcgtgg taacggaaca ggcagtgagc
    12421 acaagagcag ccagcggtag caggcggata agacgaaaat cgggcagggg catagtgatg
    12481 acaagtcctt gagatacgtt gcagttataa cccttaatgc tagcgttacc gtccgctatc
    12541 gtctatgttc aagttgtctt aattgccaga atctaacggc tttcggcaat tactccaaaa
    12601 gggggcgctc tcttttattg atcttacgca tcctgtatga tgcaagcaga ctaaccctat
    12661 caacgttggt attatttccc gcagacatga cccttttagc actcggtatc aaccataaaa
    12721 cggcacctgt atcgctgcga gaacgtgtat cgttttcgcc ggataagctc gatcaggcgc
    12781 ttgacagcct gcttgcgcag ccgatggtgc agggcggcgt ggtgctgtcg acgtgcaatc
    12841 gcacggaact ttatcttagc gttgaagagc aggataacct gcaagaggcg ttaatccgct
    12901 ggctttgcga ttatcacaat cttaatgaag aagatctgcg taaaagcctc tactggcatc
    12961 aggataacga cgcggttagc catttaatgc gtgttgccag cggcctggat tcactggttc
    13021 tgggggagcc gcagatcctt ggtcaggtta aaaaagcgtt tgccgattcg caaaaaggcc
    13081 atatgaaggc cagcgaactg gaacgcatgt tccagaaatc tttctctgta gcgaaacgcg
    13141 ttcgcactga aacagatatc ggtgccagcg ctgtgtctgt cgcttttgcg gcttgtacgc
    13201 tggcgcggca gatctttgaa tcgctctcta cggtcacagt gttgctggta ggcgcgggcg
    13261 aaaccatcga gctggtagcg cgtcatctgc gcgaacataa agtacagaag atgattatcg
    13321 ccaaccgcac tcgcgaacgt gcccaaattc tggcagatga agtcggcgcg gaagtgattg
    13381 ccctgagcga tatcgacgaa cgtctgcgcg aagccgatat catcatcagt tccaccgcca
    13441 gcccgttacc gattatcggg aaaggcatgg tggagcgcgc attaaaaagc cgtcgcaacc
    13501 aaccaatgct gttggtggat attgccgttc cgcgcgatgt tgagccggaa gttggtaaac
    13561 tggcgaatgc ttatctttat agcgttgacg atctgcaaag catcatttcg cacaacctgg
    13621 cgcagcgtaa agccgcagcg gttgaggcgg aaactattgt cgctcaggaa accagcgaat
    13681 ttatggcgtg gctgcgagca caaagcgcca gcgaaaccat tcgcgagtat cgcggccagg
    13741 cagagcaagt tcgcgatgag ttaaccgcca aagcgttagc ggcccttgag cagggcggcg
    13801 acgcgcaagc cattatgcag gatctggcat ggaaactgac taaccgcttg atccatgcgc
    13861 caacgaaatc acttcaacag gccgcccgtg acggggataa cgaacgcctg aatattctgc
    13921 gcgacagcct cgggctggag tagcagtaca tcattttctt tttttacagg gtgcatttac
    13981 gcctatgaag ccttctatcg ttgccaaact ggaagccctg catgaacgcc atgaagaagt
    14041 tcaggcgttg ctgggtgacg cgcaaactat cgccgaccag gaacgttttc gcgcattatc
    14101 acgcgaatat gcgcagttaa gtgatgtttc gcgctgtttt accgactggc aacaggttca
    14161 ggaagatatc gaaaccgcac agatgatgct cgacgatcct gaaatgcgtg agatggcgca
    14221 ggatgaactg cgcgaagcta aagaaaaaag cgagcaactg gaacagcaat tacaggttct
    14281 gttactacca aaagatcctg atgacgaacg taacgccttc ctcgaagtcc gtgccggaac
    14341 cggtggcgac gaagcggcgc tgttcgcagg cgatctgttc cgtatgtaca gccgttatgc
    14401 cgaagcccgc cgctggcggg tagaaatcat gagcgccagc gagggtgaac atggtggtta
    14461 taaagagatc atcgccaaaa ttagcggtga tggtgtgtat ggtcgtctga aatttgaatc
    14521 cggcggtcat cgcgtgcaac gtgttcctgc tacggaatcg cagggtcgta ttcatacttc
    14581 tgcttgtacc gttgcggtaa tgccagaact gcctgacgca gaactgccgg acatcaaccc
    14641 agcagattta cgcattgata ctttccgctc gtcaggggcg ggtggtcagc acgttaacac
    14701 caccgattcg gcaattcgta ttactcactt gccgaccggg attgttgttg aatgtcagga
    14761 cgaacgttca caacataaaa acaaagctaa agcactttct gttctcggtg ctcgcatcca
    14821 cgctgctgaa atggcaaaac gccaacaggc cgaagcgtct acccgtcgta acctgctggg
    14881 gagtggcgat cgcagcgacc gtaaccgtac ttacaacttc ccgcaggggc gcgttaccga
    14941 tcaccgcatc aacctgacgc tctaccgcct ggatgaagtg atggaaggta agctggatat
    15001 gctgattgaa ccgattatcc aggaacatca ggccgaccaa ctggcggcgt tgtccgagca
    15061 ggaataatgg aatatcaaca ctggttacgt gaagcaataa gccaacttca ggcgagcgaa
    15121 agcccgcggc gtgatgctga aatcctgctg gaacatgtta ccggcaaagg gcgtactttt
    15181 atcctcgcct ttggtgaaac gcagctgact gacgaacaat gtcagcaact tgatgcgcta
    15241 ctgacacgtc gtcgcgatgg tgaacccatt gctcatttaa ccggggtgcg agaattctgg
    15301 tcgttgccgt tatttgtttc gccagcgacc ttaattccgc gcccggatac ggagtgtctg
    15361 gtggagcagg cactggcgcg gttgcctgaa caaccttgcc gtattctcga tctcgggacg
    15421 ggtaccgggg cgattgcgct tgcgctggct cgcgagcgcc cggactgcga aattaccgct
    15481 gtagatcgta tgcccgatgc tgtcgccctg gcacaacgta atgcccagca tctggcgatc
    15541 aaaaatatcc acattctgca aagcgactgg tttagcgcgc tagccgggca gcagtttgcg
    15601 atgattgtca gcaatccgcc gtatattgac gagcaggacc ctcatcttca acaaggcgat
    15661 gtccgctttg agccgctcac tgcgctggtt gcggcagaca gtggaatggc agacatcgtg
    15721 catatcatcg aacagtcgcg taacgcgctg gtatccggcg gctttctgct tctggaacat
    15781 ggctggcagc agggcgaagc ggtgcggcag gcatttatcc tcgcggggta tcatgacgtc
    15841 gaaacctgcc gtgactatgg tgataacgag cgcgtaacgc tcggccgcta ttatcaatga
    15901 caagtttttc tacactgctt agtgttcatc ttattagtat cgcgctttct gttgggctat
    15961 taaccttacg tttctggcta cgttatcaga agcatccaca ggcatttgct cgctggacgc
    16021 gcattgtgcc gccggttgtc gatacgatgt tattgttaag cggcattgcg ttgatggcta
    16081 aagcgcacat cctgccattt tctgggcagg cacagtggct gactgaaaag ctgtttggag
    16141 ttatcattta tatcgttttg ggttttattg cactcgatta tcgtcgtatg cacagtcagc
    16201 aggcgcgcat tattgccttc ccgctggcgt tggtggtgct gtacatcatc attaaactcg
    16261 ccaccacaaa agtaccgtta ctggggtaag tcatgagatc gttagctgat ttcgaattta
    16321 ataaagcgcc attgtgcgaa ggcatgatcc tggcttgcga agcaatccgc cgcgattttc
    16381 cctcgcaaga tgtttacgac gaactggagc gtctcgttag tctggcgaag gaagaaatca
    16441 gccagcttct gcctttagaa gagcagctgg aaaaactgat cgcgctgttt tacggcgact
    16501 ggggatttaa agcctcacgc ggcgtttatc gtctttccga tgcattatgg ctggaccagg
    16561 tgttaaagaa tcgacagggc agtgcggtat cattaggtgc ggttttatta tgggtcgcga
    16621 atcgtctcga tttgccgctg ctgccggtga ttttccctac gcagctgata ttgcgcattg
    16681 aatgtccgga tggcgaaatt tggctgatta atccttttaa cggtgaatcg ttaagcgaac
    16741 atatgctgga cgtatggtta aagggaaata tcagcccgtc ggcggaactg ttttatgaag
    16801 accttgatga agctgataac attgaggtaa tccgcaaatt gctggataca ctcaaagcct
    16861 cgttgatgga agaaaatcag atggagctgg cgttacgcac cagcgaagct ttattacaat
    16921 tcaaccctga agatccctat gaaattcgcg atcgcgggtt gatttatgcg caactggatt
    16981 gcgaacacgt tgcgttgaac gatttaagtt atttcgttga acagtgtccg gaagacccga
    17041 tcagcgaaat gatccgtgcg caaataaata acatcgcgca taaacatatt gtgctgcatt
    17101 aattaatcga cattttactc aagattaagg cgatcctatg aaacaaaaag tggttagcat
    17161 tggcgacatc aacgtagcaa atgacctgcc gttcgtactg tttggtggta tgaacgtgct
    17221 ggaatctcgc gatctggcga tgcgcatttg cgagcactac gtaaccgtga cccagaaact
    17281 gggtatccct tacgtgttca aagcctcttt tgacaaagcc aaccgctcct ccatccactc
    17341 ttatcgtgga ccgggcctgg aagaagggat gaaaatcttc caggaattga agcagacttt
    17401 tggcgtgaaa attatcaccg acgttcacga accaagtcag gcacagcccg ttgctgatgt
    17461 cgtggatgtg attcagttgc cggcgtttct tgctcgccag actgacctgg ttgaagccat
    17521 ggcgaaaacc ggtgcggtaa ttaacgtcaa gaaaccacag tttgtcagcc cgggacagat
    17581 gggtaatatc gttgataaat tcaaagaagg cggcaacgaa aaagtgattc tttgcgatcg
    17641 cggtgctaac ttcggctatg acaacctggt tgtcgatatg ctgggcttca gcattatgaa
    17701 gaaagtgtct ggtaactcgc cggtgatttt cgacgtgacc cacgcactgc aatgccgcga
    17761 tccttttggc gcagcttccg gcggtcgtcg tgctcaggtg gctgagctgg cacgagccgg
    17821 tatggcggta ggtctggcgg ggctgtttat tgaagcgcat ccggatccgg aacatgcgaa
    17881 atgtgatggt ccatccgcgc tgccgctggc taaactggaa ccgttcctca agcagatgaa
    17941 agcgattgat gatctggtga aaggtttcga agaactggat accagcaagt aatctttttg
    18001 tttgaaaaat aaagtcttag cgttctgcgt taagactttt tttatgggtg ccggatacaa
    18061 aaaaggccgc aggctgttac ccctgcggcc ggtttcgggc gcatattgcc atcacggcag
    18121 cctgacgccc gttttcgcct tacttccggt tacgccacca gccgacaatc gctgcggtaa
    18181 taattcccgc caggatcggt gctgccaggt cgtgccagaa aatcatggca aactgcgcga
    18241 gcgtcatata gccgccttgt tgtaatgaca acattttgcg gctattcttg aattgttgat
    18301 ggttcaagat tagcccccgt tctgttgtca ggttttacct ctcaacgtgc gggggttttc
    18361 tctttccagc aaccaatgcc accagggata aagcccccgc aacattgcgc ctcaccggat
    18421 aacgccggct tggtgtggat actacgactc aattcatctt cacttcatcc ctgaaatgtt
    18481 tgcaatgaag agtgcattcc ggtttttcaa cagctgttac agtcgtttca tgagtgctct
    18541 ggatgatgct tccagctcgg gttgccaata tttacttgtg gaagtgataa agacaaaaat
    18601 ggccgcaggc tgttacccct gcggccggtt tcgggcgcat attgccatca cggcagcctg
    18661 acgcccgttt tcaccttact tccggttacg ccaccagccg acaatcgctg cggtaataat
    18721 tcccgccagg atcggtgccg ccaggtcgtg ccagaaagtc atggcaaact gcgcgagcgt
    18781 catatagccg ccttgttgta atgacaacat tttgcggcta ttcttgaagt gtctggtttc
    18841 aagattagcc cccgttttgt tgtcaggttt tacctctcaa cgtgcggggg ttttctcttt
    18901 ccagcaacca atgccaccag ggataaagcc cccgcaacat tgcgcctcac cggataacgc
    18961 cggcttggtg tggatactac gactcaattc atcttcactt catccctgaa atgtttgcaa
    19021 taaagagtac attccggctt ttcaacagct gttgcagtgg tttcgtgcag gttgtggtac
    19081 aggctcgcaa ttctgattaa cgacctgtcc aggtagtatg aagaaataca ataagcagga
    19141 atttatatta tctcccccga tttccgtcat cggaggagat ataactgtca ggcaaatatc
    19201 gtcatcaaat aggcggcaaa cagtgccaga tgcgctgcgc cattgagcac gttagtacgt
    19261 ccggtggaga aggagatatg gcacagcact aaagaggcca ccatcaccac catttctggc
    19321 gcaccaagtg caaactgtaa ttcgttaccc gtcataaagg caattagcgt gacgacaggt
    19381 acggtaagcg aaatggttgc taacactgaa ccaaagaaca gattcatcgc gcgctggacc
    19441 tggttgttca acactgcttt taacgcacct aaaccttccg gcgacagaat caacagagcc
    19501 accaggaagc cagtaaaggc gacaggggca ttcatgctgt cgagcaatgt ctccagcggg
    19561 ctggcattca ttttggtcac cgcaataacg gcaatcagat ggataatcaa ccagatagca
    19621 tgccacacgc tgctatgggc agacggttta ccgtgatgcg ggtcatcgtc atcactgtca
    19681 tcttcgtgct cgtagacaaa caaactttga tgcgttttgg tctggatcag caaaaatacg
    19741 ccatacattg ccgcagaaat taatgctacc agtaacgcct gaccggttga aaaattcgcc
    19801 gcaggcagag ccattggaaa taccagtacg attatcgcca gggggaacag cgcaattaaa
    19861 tactgcttga taccaaacag attcatatat tgggtggcaa acttacgacc gcccaacaat
    19921 aatgaaaagc caaccagccc accggtaaca atcataatga ttgaatagag cgtatcacgc
    19981 attagcgttg gcgcggcgtc gccggttgcc attaaagctg aaatcaaact gacttcaaga
    20041 ataaccactg aaaggctaag aataagcgaa ccgtaaggtt ctcccaggcg atgggctaat
    20101 acgtccgcat gacggacaac actaaaggcg ctacttaaaa taccaataag cgcaagaaga
    20161 ttgatggcaa tgaccactgg tagtgtctgg ctgcttcccc acaggaacag cactaccagc
    20221 gccagaaccg ggaaaataag cgaagtctcc ttgtggcggg tttttaccgc ctcttgagca
    20281 tttgacatta tggttatccc tttgcagatg aatttatcga aaatgtaaaa aataggtagg
    20341 aaaaataaca gaaagtgtct ggatatcggt aacattttac gaatttttac cccactgtcg
    20401 attttttact cattgggaca taaaaataag tattacgttt agacaatgtt tgttttagcg
    20461 tttcttaaga acagtctgac atgaaaattc ttatgttttg gcaagagtag atattgttga
    20521 ccacacttaa tgttcaactt tgtaaaagga gtcaacgatg ccgtataaaa cgaaaagcga
    20581 tctgccggaa agcgtaaagc acgttctacc gtctcatgcc caggatatct ataaagaagc
    20641 gttcaacagc gcatgggatc aatataaaga taaagacgat cggcgtgatg acgccagtcg
    20701 cgaagaaacc gcgcataaag tggcctgagc tgctgtgaag catgaatatg ccaaagggga
    20761 tgatgataaa tggcataaaa aatcgtaaaa ccggtagctt agttaaagct attcgtgcgg
    20821 tgttgccttg caagtggtcc gtggattgca tattgtcccg ttagtggttt caaaatgagc
    20881 agtaaaaatg tccggaagac accaaaaagt tgtcgcaggg aagtatgcag tggcggaggt
    20941 gtaaggtgat aacgcgtgat ttcttgatga atgccgattg taaaacggca tttggtgcca
    21001 ttgaagaatc actcttatgg tcagcagagc aacgggcggc ttcgctggcg gcgacgctgg
    21061 cttgtcgacc tgatgaggga ccggtgtgga tcttcggtta tggatcgttg atgtggaatc
    21121 cggcactgga gtttaccgag tcgtgcaccg gtacactggt tggatggcat cgcgcgtttt
    21181 gcctgcgcct gaccgccggg cgcgggactg cgcaccagcc gggacggatg cttgcactga
    21241 aagagggcgg acgcactaca ggcgtcgcct atcgactgcc agaagagacg ctggagcagg
    21301 aactaaccct gttgtggaag cgcgagatga ttaccggctg ttatctgcca acctggtgtc
    21361 agcttgatct tgatgatgga tgcacagtaa acgccattgt gtttattatg gacccgcgac
    21421 atccagaata tgaatctgac actcgcgctc aggtcatcgc gccgttgatt gcggcggcga
    21481 gcggtccgct gggaaccaac gcacaatacc tgttttcact ggaacaggag cttatcaaac
    21541 tgggaatgca ggacgatggg ctgaatgatt tgctggtatc ggtaaaaaaa ctgctggcgg
    21601 agaattatcc ggatggtgtg ttacggccgg gattcgcctg agtaaacttc cctcatagtg
    21661 gggcgtcaga cgccccctca aacattaaaa tgtgagcact ttatcggctg acagcgtcca
    21721 ttgcgccagt tccacaagag taccgatttc caccccatca ataagaggaa gtgtgctaat
    21781 cccgcgcccg tcggtacagg ttttgcacaa tttcaccggt acattctgag cggtaaggat
    21841 ctccagcatt tgctgaatgt tgtagccttc ccctggtttt tgcccgcgca acccggctgt
    21901 gaccgcatca gacatcagga acagacgcag atccagattg ctctcctgct ctcgtaacgc
    21961 aatggccagc cgcaagctgt taaacaagga ttcgctcccg taaggtgcgc cattggcaac
    22021 gatcacgatt ttttgcatta tttactcctg tattcaggga attagacatt catcttctat
    22081 cttactgctt ctgcagcgtc tgaccaatcg gtcacatttt taaggatttt cctgaaagcg
    22141 cgagaaaata cgacaaaagt tgccagtaat cgttattctt taaggctatg gtttttcatt
    22201 attaccggaa gttaccgacg ttttgagccg tttcgttcct cgcattatcc cgttttattt
    22261 actcttgctt gtagcaggcg gtacagctaa cgcacaatct accttcgagc aaaaagcggc
    22321 aaatcccttt gataataaca atgatggtct gccggattta ggcatggctc ccgaaaatca
    22381 tgatggggaa aaacactttg ctgaaattgt gaaagatttc ggcgaaacca gtatgaatga
    22441 taacgggctg gatactggcg agcaggcaaa agctttcgca ttgggaaaag tccgagacgc
    22501 gcttagtcaa caggttaatc agcacgtaga gtcctggcta tcaccgtggg gaaatgccag
    22561 tattgacgtc aaagtggata acgaaggaca tttcaccggc agtcgtggaa gctggtttgt
    22621 gccgttacaa gataatgatc gttatctcac ctggagccag cttggtctta ctcagcagga
    22681 tgatgggttg gtgagcaatg tgggcgttgg gcaacgctgg gcgcgcggca actggctggt
    22741 gggttataac actttttatg acaacttgct ggacgaaaat cttcagcgag cgggctttgg
    22801 tgccgaagcg tggggcgaat atttgcgact atcggcaaac ttttatcagc cgtttgctgc
    22861 atggcatgaa cagacagcca cgcaggaaca acggatggcg cgcgggtatg acctgacagc
    22921 ccggatgcgc atgccgttct atcaacacct caataccagt gtcagcgtag aacagtattt
    22981 tggtgagcgt gttgatttgt ttaactctgg tacgggttat cacaatcccg tcgcgttgag
    23041 tctgggatta aattacaccc ctgtgccatt agtcactgtg acggcccagc ataaacaggg
    23101 tgaaagtggc gagaatcaaa ataacctcgg gctgaatctt aattaccgct ttggtgtacc
    23161 gctcaaaaaa caactttctg cgggcgaggt tgccgaaagt cagtcgttac gtggtagtcg
    23221 ctatgataat ccgcagcgaa ataatctacc gactcttgag taccgacagc gaaaaacgtt
    23281 aacggtgttt ctggcgacac cgccgtggga tctaaaacct ggcgaaacag tgccgctgaa
    23341 attacaaatc cgcagtcgtt atggtattcg gcaactgatt tggcagggcg atacgcagat
    23401 attaagtttg acgccaggcg cacaagccaa cagcgcggag ggctggacgc tgatcatgcc
    23461 tgactggcag aacggggaag gggcgagcaa tcactggcga ttgtcggtag tggtggaaga
    23521 taaccagggg cagcgtgtct cctccaatga gatcacgcta acgcttgtcg aaccgttcga
    23581 cgcattgtca aacgacgaac tgcgctggga accgtaatca gaaaatgcgc tcctgatgca
    23641 cccataccgc tgcttccacg cgagacttga gcttcatttt cttcagcatg tgcttgacgt
    23701 gcacttttac tgtgctttcg gtgatgtcca ggcggcgggc aatcatcttg ttcggcaaac
    23761 cctgggcaat cagcttgaga atatcgcgct cgcgtggggt taactggtta acatcgcgtt
    23821 cagtagtggc acggttagcg cgcaagctgg ctgccagaac aggcgttaat gcctcgctta
    23881 ataccatttc gccagcagct gcctgatgca atgctttcag cagatcttcc ggttccatat
    23941 cttttaatag atagccatcc gcgccgcgtt tcagtgcggt gaccacatct tcttcatggt
    24001 tagagacgct gaataccaca atgcgccctg agagggactt ttcgcgcagt ttatccagcg
    24061 tttccagacc gttcatgccg ggcatattga gatctaacag gatcagatcg ggatcaagag
    24121 attccgccag ttcaataccc tgttcgccat tactcgcttc gccaactacg gtgatatctg
    24181 gtgccatact gataagctgt ttcacgccag ttcgcagcat cggatgatcg tcgatcagca
    24241 ggatagtagc cggttcctga ttactcatgg gtatctcctt ggacgtctgt gaaagttttt
    24301 tcgggaataa aggtgacaac cacttcggtg ccacctgatt cacgacggcg aacgcggcaa
    24361 tcgcctcgta aactttgcgc acggtcgcgc attattatca tgccgtagtg attgctgcgg
    24421 atggcatttt caggcacgcc gcagccgtta tcctggacgg tcagtttgac ctgattatcg
    24481 ttttgcgcca ccgtcaccac gacctcactc gcttgcgaat gtttgagggc gttacttaat
    24541 gcctcacggg caatttgcaa caagtggatt gcctgatgcg aaggcaccag acgaggcggc
    24601 aattgataat ccagcttcac cgggaagcca aatttggcgc tgtactcttc gcaactcgcc
    24661 tccagcgccg gacgtaatcc aggctcggtg agctgcaagc ggaatgtggt gagcaattca
    24721 cgcaactgcg cccaggatgc attcagttcg ttacggatct gacttaacag ttcgcggctg
    24781 ctttctggca gcgcatcgcc ctgcatctgt aaacaactca cctgcatctt catgcaagag
    24841 agagattggg caatagaatc atgcagttcg cgcgcaatgg tggcacgctc ttccatcacg
    24901 atcaactgtt gctgacgttc ctgatgccga tccagcgcca gcgtggcggt gagttgttca
    24961 accagggtat ccaccagttg ttgttgatca tggctaagat gacgcccctg cggcagggtc
    25021 gccagcaaaa taccgtactg cgtatgagag tcagccagcc gccacttcag ggtcgtgccg
    25081 cgatcaccaa cgggtaatac gccgcgcggg cagagctggc agcctttatc atcacaagtc
    25141 atatctggct ggcaggtaaa ctcctgatga ttctcttcat catcagtgtc atacacccgc
    25201 agttcgatat cacgtagcag ggttaaattc tgtaagccgt tgagtacagg tgacaggcgt
    25261 tcacacagcg gggcgcggga atgcaaacgg cggttagcct gccataaaaa agagaggatc
    25321 tgatttttat gctccagccc ggcggttttc tcctgaaccc gctgctcaag tacggcataa
    25381 ctttcggcca gttctgcaga catattgttc aacgcagttc caagcatcgc catttcgttg
    25441 cgcccgctga tgtttgcgcg ttgggtaaaa tcgcgatggc tgacggcact cgccattgcc
    25501 agcagttgcc gccacggttg tagcagtcgc gcccgcaacc agataatagt gaacaccagt
    25561 aaaagtgcca taaataccgc cattacccga tggaccagta ccactgtctc aatgcgcatt
    25621 tccgtggtgc ggtcaaaacc agataccagc tgatcaagcc cggcaacaaa ctggctgaca
    25681 tccgctgaca ccgtttctcg gttttgtgca cgcatcagcg cagggatcag ttcattacgc
    25741 cagtaatctt gtaaaccctg taattgcgcc agttgtccgt ctcgttctgc tgctcgagtc
    25801 aactcggcgc taaatgccgt ttgttccatc tctttaatta agggcttgtc tttctcgctt
    25861 aatggcactg ccgccaacag acggtaactt tgcatgcgca gcgatcccgc tttgttgatc
    25921 gcatgggcgc tgccctgaac gccttgcacc agccagccag aaaccgccat ccctgccagt
    25981 ccaatagcag tagaaagcaa cacaataagc gcaacctgat taaccagggt gagcggagag
    26041 agacaacgtt taagcatgta aacctcttcc ttcaggcttt aaatgagcaa taaccttaat
    26101 gaatgtgacg atacaatctg gaatggcagt attctcggct attggctgaa gtatacccat
    26161 acccggaaag agttactcct tatttgccgt gtggttagtc gctttacatc ggtaagggta
    26221 gggattctac agcaccgtga aaaatctcat aatttttatg aaatcactgt actcactatg
    26281 ggtaatgata aatatcaatg atagataaag ttatcttatc gtttgattta catcaaattg
    26341 cctttagcta cagacactaa ggtggcagac atcgaaacga gtatcagagg tgtctatgag
    26401 tcactcatcc gcccccgaaa gggctactgg agctgtcatt acagattggc gaccggaaga
    26461 tcctgcgttc tggcaacaac gcggtcaacg tattgccagc cgcaacctgt ggatttccgt
    26521 tccctgtctg ctgctggcgt tttgcgtatg gatgttgttc agcgctgttg cggtgaactt
    26581 accgaaagtc ggttttaatt ttacgaccga tcagctattt atgttgactg cgctgccttc
    26641 ggtttctggc gcgttattac gtgttccata ctcctttatg gttcctatct tcggtggtcg
    26701 tcgctggacg gcgttcagca ccggtattct gattattcct tgtgtctggc tgggttttgc
    26761 cgtgcaggat acctccacgc cttatagcgt cttcatcatc atctctctgc tgtgcggctt
    26821 tgctggcgcg aacttcgctt ccagtatggc aaacatcagc ttcttctttc cgaaacagaa
    26881 gcagggtggc gcgctgggtc tgaatggtgg tctgggcaac atgggcgtca gcgtcatgca
    26941 gttggttgct ccgctggtgg tatcactgtc gattttcgca gtatttggta gccagggcgt
    27001 caaacagccg gatgggactg agctgtatct ggcgaatgcg tcctggatat gggtgccgtt
    27061 ccttgccatc ttcaccattg cggcgtggtt tggcatgaac gatcttgcta cctcgaaagc
    27121 ctccatcaag gagcagttgc cggtactcaa acggggtcat ctgtggatta tgagcctgct
    27181 gtatctggca accttcggct cctttatcgg cttctccgcg ggctttgcga tgctgtcaaa
    27241 aacgcagttc ccggatgttc agattctgca atacgctttc ttcgggccgt ttattggtgc
    27301 gctggcgcgt tctgcaggtg gtgcattatc tgaccgtctg ggcggaactc gtgtcacgct
    27361 ggtgaacttt attctgatgg cgattttcag cggcctgctg ttcctgacct taccgactga
    27421 cgggcagggc ggaagcttca tggcgttctt cgcggtcttc ctggcgctgt tcctgacagc
    27481 tgggctgggt agtggttcca ctttccagat gatttcagtg atcttccgta aactgacaat
    27541 ggatcgcgtg aaagcagaag ggggttctga cgaacgtgcg atgcgtgaag cggcaaccga
    27601 cacggcggcg gcgctgggtt tcatctctgc gattggcgcg attggtggct tctttatccc
    27661 gaaagcgttt ggtagctcgc tggcattaac gggttcgcca gtcggcgcaa tgaaggtatt
    27721 tttgattttc tatatcgcct gcgtagtgat tacctgggcg gtatatggtc ggca
//
LOCUS       DAJOWB010000054        27559 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJOWB010000054 DAJOWB010000000
VERSION     DAJOWB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.054"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..96
                     /locus_tag="OHO85_002491"
     CDS             <1..96
                     /locus_tag="OHO85_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCQ2671852.1"
                     /translation="SSQECPPGESKITNKCLHNSCIIKICPYGLK"
     gene            complement(199..1449)
                     /gene="icd"
                     /locus_tag="OHO85_002492"
     CDS             complement(199..1449)
                     /gene="icd"
                     /locus_tag="OHO85_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCQ2671853.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1621..2274
                     /gene="rluE"
                     /locus_tag="OHO85_002493"
                     /pseudo
     CDS             1621..2274
                     /gene="rluE"
                     /locus_tag="OHO85_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
     gene            2284..2745
                     /gene="nudJ"
                     /locus_tag="OHO85_002494"
     CDS             2284..2745
                     /gene="nudJ"
                     /locus_tag="OHO85_002494"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCQ2671854.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2799..3905
                     /gene="mnmA"
                     /locus_tag="OHO85_002495"
     CDS             2799..3905
                     /gene="mnmA"
                     /locus_tag="OHO85_002495"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCQ2671855.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3941..4582
                     /gene="hflD"
                     /locus_tag="OHO85_002496"
     CDS             3941..4582
                     /gene="hflD"
                     /locus_tag="OHO85_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCQ2671856.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4586..5956
                     /gene="purB"
                     /locus_tag="OHO85_002497"
     CDS             4586..5956
                     /gene="purB"
                     /locus_tag="OHO85_002497"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCQ2671857.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6125..6796
                     /gene="phoP"
                     /locus_tag="OHO85_002498"
     CDS             6125..6796
                     /gene="phoP"
                     /locus_tag="OHO85_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCQ2671858.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHI
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6796..8256
                     /gene="phoQ"
                     /locus_tag="OHO85_002499"
     CDS             6796..8256
                     /gene="phoQ"
                     /locus_tag="OHO85_002499"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCQ2671859.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8332..9453
                     /gene="roxA"
                     /locus_tag="OHO85_002500"
                     /gene_synonym="ycfD"
     CDS             8332..9453
                     /gene="roxA"
                     /locus_tag="OHO85_002500"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCQ2671860.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9502..10728)
                     /gene="pepT"
                     /locus_tag="OHO85_002501"
     CDS             complement(9502..10728)
                     /gene="pepT"
                     /locus_tag="OHO85_002501"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCQ2671861.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVESYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            10978..12114
                     /gene="potA"
                     /locus_tag="OHO85_002502"
     CDS             10978..12114
                     /gene="potA"
                     /locus_tag="OHO85_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCQ2671862.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12098..12955
                     /gene="potB"
                     /locus_tag="OHO85_002503"
     CDS             12098..12955
                     /gene="potB"
                     /locus_tag="OHO85_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCQ2671863.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12952..13746
                     /gene="potC"
                     /locus_tag="OHO85_002504"
     CDS             12952..13746
                     /gene="potC"
                     /locus_tag="OHO85_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCQ2671864.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13743..14789
                     /gene="potD"
                     /locus_tag="OHO85_002505"
     CDS             13743..14789
                     /gene="potD"
                     /locus_tag="OHO85_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCQ2671865.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            14847..15308
                     /locus_tag="OHO85_002506"
     CDS             14847..15308
                     /locus_tag="OHO85_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HCQ2671866.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            15305..16093
                     /locus_tag="OHO85_002507"
     CDS             15305..16093
                     /locus_tag="OHO85_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCQ2671867.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(16232..17053)
                     /gene="cobB"
                     /locus_tag="OHO85_002508"
     CDS             complement(16232..17053)
                     /gene="cobB"
                     /locus_tag="OHO85_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCQ2671868.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(17069..17980)
                     /gene="nagK"
                     /locus_tag="OHO85_002509"
     CDS             complement(17069..17980)
                     /gene="nagK"
                     /locus_tag="OHO85_002509"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCQ2671869.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(18009..19253)
                     /gene="lolE"
                     /locus_tag="OHO85_002510"
     CDS             complement(18009..19253)
                     /gene="lolE"
                     /locus_tag="OHO85_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCQ2671870.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(19253..19954)
                     /gene="lolD"
                     /locus_tag="OHO85_002511"
     CDS             complement(19253..19954)
                     /gene="lolD"
                     /locus_tag="OHO85_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCQ2671871.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(19947..21146)
                     /gene="lolC"
                     /locus_tag="OHO85_002512"
     CDS             complement(19947..21146)
                     /gene="lolC"
                     /locus_tag="OHO85_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCQ2671872.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            21408..22481
                     /locus_tag="OHO85_002513"
     CDS             21408..22481
                     /locus_tag="OHO85_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCQ2671873.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLLYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNVARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            22609..26055
                     /gene="mfd"
                     /locus_tag="OHO85_002514"
     CDS             22609..26055
                     /gene="mfd"
                     /locus_tag="OHO85_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCQ2671874.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRMFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARILLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            26199..27161
                     /gene="ldtC"
                     /locus_tag="OHO85_002515"
     CDS             26199..27161
                     /gene="ldtC"
                     /locus_tag="OHO85_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCQ2671875.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(27244..27501)
                     /gene="bhsA"
                     /locus_tag="OHO85_002516"
     CDS             complement(27244..27501)
                     /gene="bhsA"
                     /locus_tag="OHO85_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCQ2671876.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6744 a         7129 c         6956 g         6730 t
ORIGIN      
        1 agcagtcaag agtgtcctcc aggagaaagc aaaataacaa ataaatgttt gcataatagt
       61 tgcattataa aaatatgccc atatgggctc aagtaaaggg ttttatataa atatgctcga
      121 tatgctatta ataatatcca cataaataaa acaacgggcg tgttatacgc ccgtttcaat
      181 atttaacaca tggagagatt acatattctt gatgatcgcg tcaccaaact ctgaacattt
      241 cagcaattta gcgccttcca tcagacgttc gaagtcatag gttacggtct tggcattgat
      301 tgcgccttcc atacctttaa caatcaggtc agccgcttca gtccaaccca tatggcgtaa
      361 catcatctca gcagagagaa taatagagcc tgggtttact ttgtcctgac cggcatattt
      421 cggcgcagta ccgtgggtgg cttcaaacag ggcgcattcg tcaccgatgt ttgcacctgg
      481 ggcgataccg ataccgccaa cctgcgctgc cagggcgtca gaaatgtagt caccgttcag
      541 gttcatacag gcgataacat catattcagc cggacgcagc aggatctgtt gcaggaatgc
      601 atcagcaatc acgtctttaa tgacgatctc tttgccggtg ttcgggtttt taactttcag
      661 ccacgggccg ccgtcgatca gttcaccgcc aaactcttca cgcgccagct ggtagcccca
      721 gtctttaaac gctccttcgg tgaacttcat gatgttgcct ttgtgcacca gagtcacaga
      781 gtcacgatcg ttagcaattg cgtattcgat cgctgcacga accagacgtt tggtgccttc
      841 ttccgaacac ggcttaatac cgataccaca atgttccggg aagcgaattt tcttcacccc
      901 catctcttca cgcaggaatt taatcacttt ctcggcgtcg gcagagtctg ctttccattc
      961 gatacccgca taaatgtctt ccgagttttc acggaagata accatatcgg tcagttcagg
     1021 gtgtttaacc gggcttggag tgccctgata gtaacgtacc ggacgcaggc agatgtagag
     1081 atccagttcc tggcgcaggg caacgttcag agagcgaata ccgccaccaa caggagtggt
     1141 cagcggacct ttaatggcaa cgcgatattc acgaatcaga tcaagggttt cagcaggcag
     1201 ccagacatcc tgaccataaa cctgtgtgga tttttcaccg gtgtaaattt ccatccagga
     1261 gattttacgc tcgcctttat aggctttctc gactgcagcg tcgaccactt tcagcatggc
     1321 tggggttaca tctacaccga ttccatcacc ttcaatgtaa gggataatcg gattttcagg
     1381 aacgttgagt ttgccgtttt gcagggtgat cttcttgcct tgtgccggaa caactacttt
     1441 actttccatt cacctctcct tcgagcgcta ctggtttgct cgtctgccac cacgttgcat
     1501 atgcgtttgc gtcctgcgat acggatgctt tagagcaatt ttttgttaat gatttgtaat
     1561 tggcttgtca atactaccct attgtttgcc gtcatgaaag atgcgcgtta ttaggctata
     1621 atgcggcaat tcataatctc tgaaaatacc atgcaaaaaa cttcttttag aaatcactag
     1681 gttaagcgat tcagctcgca acgttctacc aggcgtaaac ctgaaaacca gcccacgcgt
     1741 gtgatcctgt tcaataaacc ctacgatgtt cttccgcagt tcaccgatga agccggacgc
     1801 aaaacattaa aagaattcat cccggttcag ggcgtttatg cagcaggtcg ccttgaccgc
     1861 gatagcgaag ggttgctggt gctgaccaat aacggcgcgt tgcaggcgcg tttaacccag
     1921 ccgggtaaac gcaccggaaa aatctattat gtgcaggtgg aaggtattcc cacacaagac
     1981 gcacttgaag ccttgcgcaa tggcgtaacc ttaaatgatg gccctaccct gcccgcaggc
     2041 gcggagctgg ttgacgaacc cgcgtggtta tggccgcgga atccaccaat tcgtgaacgc
     2101 aaaagtattc ccaccagctg gctgaagatc accttatatg aaggacgtaa tcgccaggtg
     2161 cgccgcatga ccgcccatgt tggtttcccc acgctgcgac tgattcgcta tgcgatggga
     2221 gattactctt tggataatct tgccaatggc gaatggcgag aagtgacaga ttaaggatgc
     2281 aaaatgttta aaccgcacgt taccgttgct tgcgtggtgc acgcagaagg caaattttta
     2341 gtcgttgaag agacgattaa tggtaaagcg ttatggaacc aacctgccgg gcatctggaa
     2401 gccgatgaaa ccttagtgga agccgccgcc cgtgaactgt gggaagaaac cggcatcagc
     2461 gcgcagccgc aacactttat tcgtatgcat cagtggattg cgccagataa aacgccgttt
     2521 ttgcgtttcc tctttgccat tgagcttgag caaatatgcc cgacccagcc tcatgacagc
     2581 gatatcgact gctgccgttg ggtcagcgcc gaagaaattt tacaggcgtc aaatcttcgt
     2641 tcgccgctgg tggcggaaag tattcgttgt tatcaaagcg ggcaacgtta tccgctggag
     2701 atgattggcg attttaactg gccttttaca aagggtgtca tctaaaaggc tgcgtgatag
     2761 aatacgccgc cttgaagttc aatgtcgtga gtgatccaat gtctgaaacc gcaaaaaaag
     2821 taatcgtcgg catgtccggc ggtgtcgatt cctccgtttc tgcctggctg ttgcaacaac
     2881 agggatatca ggtcgaaggc ctgtttatga agaactggga agaagacgac ggtgaggaat
     2941 attgcacagc ggcagcggat ctggctgatg cccaggctgt ctgcgacaag ctcggcattg
     3001 aactgcacac cgttaacttt gctgccgagt actgggacaa cgtcttcgaa ctgttccttg
     3061 ccgaatataa agccggtcgc acgccgaatc cggatattct gtgcaacaaa gagatcaaat
     3121 ttaaagcctt cctcgaattt gccgccgaag atttaggtgc cgattatatc gctaccggtc
     3181 attacgtacg tcgggccgat gtcgatggca agagccgcct gctgcgtggt ctggacagca
     3241 ataaagacca gagctacttc ctttatacgc tcagccatga gcagattgcg caaagcctgt
     3301 tcccggtcgg cgaactggaa aaaccgcagg tgcgtaagat tgctgaagat cttggtctgg
     3361 tcaccgcgaa gaaaaaagac tctaccggca tttgctttat tggcgaacgt aaattccgcg
     3421 agttcctggg ccgttatctc ccggcgcaac cgggcaaaat cattaccgtc gatggcgatg
     3481 aaattggtga gcaccagggg ctgatgtatc acactctcgg tcagcgtaaa ggtctgggta
     3541 tcggtggcac caaagaaggt accgaagaac cgtggtatgt ggtggacaaa gacgtcgaaa
     3601 acaacattct ggttgtcgct cagggccatg aacacccgcg gctgatgtct gtcgggttga
     3661 ttgcccagca gttgcactgg gtcgatcgcg aaccattcac cggcactatg cgttgcacgg
     3721 taaaaacccg ctatcgccag accgatatcc cttgcaccgt caaggcgctg gacgatgatc
     3781 gcattgaagt gattttcgat gaaccggttg ccgccgtgac gccgggccag tctgccgtct
     3841 tctataacgg tgaagtgtgc ctcggtggcg gtattattga gcagcgtctg ccgctgccgg
     3901 tctgattatt atctttactt aaaacaagga agcagtgaac gtggcaaaga attactatga
     3961 catcaccctc gccctggccg gtatttgtca gtcggcacgc ctggtgcaac aactcgctca
     4021 ccaggggcat tgtgatgccg atgcgctaca cgtctcactc aacagtatta ttgatatgaa
     4081 ccccagctcg acgctggcgg tttttggcgg tagcgaagcc aacctgcgcg tcgggctgga
     4141 aaccctgctc ggcgtgctca atgccagcag tcgccagggc ttaaacgccg aattaacccg
     4201 ctacacactc agcttgatgg tgcttgagcg caaactctcc tcagcgaaag gcgcgctcga
     4261 cactctgggc aaccggatca acggcctgca acgccagctc gaacacttcg atttacagtc
     4321 cgaaacgctg atgagcgcga tggctgctat ctatgttgat gtgattagcc cgcttggccc
     4381 gcgcattcag gtcaccggtt cccctgctgt actgcaaagc ccacaagtgc aggcgaaagt
     4441 tcgcgcaacc ctgctggcag gcattcgcgc cgccgtgctc tggcaccagg tcggcggcgg
     4501 acgtctgcaa ctgatgtttt ctcgtaatcg cctgaccact caggcaaaac aaattcttgc
     4561 tcatttaacc ccggagttgt gatctatgga attatcctca ctgaccgccg tttcccctgt
     4621 cgatggacgc tacggcgata aagtcagcgc gctgcgcggg attttcagcg aatatggttt
     4681 gctgaaattc cgtgtacaag ttgaagtacg ttggctgcaa aaactggccg cgcacgcagc
     4741 gatcaaggaa gttcctgctt ttgctgccga cgcaatcggt taccttgatg caatcgtcgc
     4801 cagtttcagc gaagaagatg cggcgcgcat caaaactatc gagcgtacca ctaaccacga
     4861 cgttaaagcg gttgagtatt tcctgaaaga aaaagtggcg gagatcccgg aactgcacgc
     4921 ggtttctgaa ttcatccact ttgcctgtac ttcggaagat atcaataacc tctcccacgc
     4981 attaatgctg aaaaccgcgc gtgatgaagt gatcctgcca tactggcgtc aactgattga
     5041 tggcattaaa gatctcgccg ttcagtatcg cgatatcccg ctgctgtctc gtacccacgg
     5101 tcagccagcc acgccgtcaa ccatcggtaa agagatggca aacgtcgcct accgtatgga
     5161 gcgccagtac cgccagctta accaggtgga gatcctcggc aaaatcaacg gcgcggtcgg
     5221 taactataac gcccacatcg ccgcttaccc ggaagttgac tggcatcagt tcagcgaaga
     5281 gttcgtcacc tcgctgggta ttcaatggaa cccgtacact acccagattg aaccgcacga
     5341 ctacattgcc gaactgtttg attgcgttgc gcgcttcaac accattctga tcgactttga
     5401 ccgtgacgtc tggggttata tcgcccttaa ccacttcaaa cagaaaacca ttgctggtga
     5461 gattggttct tccaccatgc cgcataaagt taacccgatc gacttcgaaa actccgaagg
     5521 gaatctgggc ctttccaacg cggtattgca gcatctggca agcaaactgc cggtttcccg
     5581 ctggcagcgt gacctgaccg actccaccgt gctgcgtaac ctcggcgtgg gtatcggtta
     5641 tgcgctgatt gcgtatcaat ccaccctgaa aggcgtgagc aaactggaag tgaaccgtga
     5701 ccatctgctg gatgaactgg atcacaactg ggaagtgctg gctgagccaa tccagacagt
     5761 tatgcgtcgc tatggcatcg aaaaaccgta cgagaagctg aaagagctga ctcgcggtaa
     5821 gcgcgttgac gccgaaggca tgaagcagtt tatcgacggt ctggcgttgc cagaagaaga
     5881 gaaagcccgc ctgaaagcga tgacgccggc taactatatt ggtcgagcta tcacgatggt
     5941 tgatgagctg aaataaacct cgtatcagtg ccggatggcg atgctgtccg gcctgcttat
     6001 taagactatc cgctttttat tttttcactt tacctcccct ccccgctggt ttatttaatg
     6061 tttaccccca taaccacata atcgcgttac actattttaa taattaagac agggagaaat
     6121 aaaaatgcgc gtactggttg ttgaagacaa tgcgttgtta cgtcaccacc ttaaagttca
     6181 gattcaggat gctggtcatc aggtcgatga cgcagaagat gccaaagaag ccgattatta
     6241 tctcaatgaa catataccgg atattgcgat tgtcgatctc ggattgccag acgaggacgg
     6301 tctgtcactg attcgccgct ggcgtagcaa cgatgtttca ctgccgattc tggtattaac
     6361 cgcccgtgaa agctggcagg acaaagtcga agtattaagt gccggtgctg atgattatgt
     6421 gactaaaccg tttcatattg aagaggtgat ggcgcgaatg caggcattaa tgcggcgtaa
     6481 tagcggtctg gcttcacagg tcatttcgct ccccccgttt caggttgatc tctctcgccg
     6541 tgaattatct attaatgacg aagtgatcaa actgaccgcg ttcgaataca ccattatgga
     6601 aacgttgata cgcaataatg gcaaagtggt gagcaaagat tcgttaatgc tacaactcta
     6661 tccggatgcg gagctgcggg aaagccatac cattgatgta ctgatgggac gtctgcgcaa
     6721 aaaaattcag gcacaatatc cgcaagaagt gattaccacc gttcgcggcc agggctatct
     6781 gttcgaattg cgctgatgaa aaaattactg cgtctttttt tcccgctctc gctgcgggta
     6841 cgttttctgt tggcaacggc agcggtagta ctggtgcttt cgcttgccta cggaatggtc
     6901 gcgctgatcg gttatagcgt cagtttcgat aaaactacgt ttcggctgtt acgtggcgaa
     6961 agcaatctgt tctataccct tgcgaagtgg gaaaacaata agttgcatgt cgagttacca
     7021 gaaaatatcg acaagcaaag ccccaccatg acgctaattt atgatgagaa cgggcagctt
     7081 ttatgggcgc aacgtgacgt gccctggctg atgaagatga tccagcctga ctggctgaaa
     7141 tcgaatggtt ttcatgaaat tgaagcggat gttaacgata ccagcctctt gctgagtgga
     7201 gatcattcga tacagcaaca gttgcaggaa gtgcgggaag atgacgacga cgcggagatg
     7261 acccactcgg tggcggtaaa cgtctacccg gcaacatcgc gaatgccaaa gttaaccatt
     7321 gtggtggtgg ataccattcc ggtggagcta aaaagttcct atatggtctg gagctggttt
     7381 atctatgtgc tctcagccaa tctgctgtta gtgatcccgc tgctgtgggt cgccgcctgg
     7441 tggagtttac gccccatcga agccctggca aaagaagtcc gcgaactgga agaacataac
     7501 cgcgaattgc tcaatccagc cacaacgcga gaactgacca gtctggtacg aaacctgaac
     7561 cgattgttaa aaagtgaacg cgaacgttac gacaaatacc gtacgacgct caccgacctg
     7621 acccatagtc tgaaaacgcc actggcggtg ctgcaaagta cgctgcgttc tctgcgtagt
     7681 gaaaagatga gcgtcagtga tgctgaaccg gtaatgctgg agcaaatcag ccgcatttca
     7741 cagcaaattg gctactacct gcatcgtgcc agtatgcgcg gcgggacatt gcttagccgc
     7801 gagctgcatc cggtcgcccc actgctggac aatctcacct cggcgctgaa caaagtgtat
     7861 caacgcaaag gggtcaatat ctctctcgat atttcgccag agatcagctt tgtcggtgag
     7921 cagaacgatt ttgtcgaggt gatgggcaat gtgctggata atgcctgtaa atattgcctc
     7981 gagtttgtcg aaatttctgc aaggcaaacc gacgagcatc tctatattgt ggtcgaggat
     8041 gatggacccg gtattccatt aagcaagcga gaggtcattt tcgaccgtgg tcaacgggtt
     8101 gatactttac gccctgggca aggtgtaggg ctggcggtag cccgcgaaat caccgagcaa
     8161 tatgagggta aaatcgtcgc cggagagagc atgctgggcg gtgcgcggat ggaggtgatt
     8221 tttggtcgcc agcattctgc gccgaaagat gaataaatat gtccatactt cacgcattac
     8281 gttaagcatc cgttataatc ggttgcagat accagcctgt ggatgcttaa catggaatac
     8341 caactcactc ttaactggcc cgattttctt gaacgtcact ggcagaaacg cccggtggtg
     8401 ttaaaacgcg gctttaataa ttttattgac ccgatctctc cagacgagtt ggcgggtctg
     8461 gcgatggaaa gcgaagtcga cagtcgactg gtcagtcacc aggatggcaa atggcaggtc
     8521 agccacggtc cgttcgaaag ctacgatcat ctcggtgaaa ctaactggtc attgttagtg
     8581 caagcagtaa atcactggca tgagccgacc gccgcgctga tgcgaccgtt ccgtgaacta
     8641 ccggactggc gtattgatga tctgatgatc tctttttctg tacctggcgg cggcgtcggc
     8701 ccgcatctcg atcagtacga cgtgtttatc attcagggta ccggacgtcg tcgctggcga
     8761 gtgggcgaaa agctgcaaat gaaacagcac tgcccacacc cggatctgtt acaggtcgat
     8821 ccgttcgaag ccatcatcga tgaagagctg gagcctggcg atattcttta tattccgcca
     8881 ggattcccgc atgaaggcta cgcgctggaa aatgcgatga actattccgt gggttttcgc
     8941 gcgccaaata cgcgggaatt aattagcgga tttgccgatt atgtgctgca acgtgaactg
     9001 ggcggcaact actacagcga tccggatgtt ccacctcgcg ctcatcctgc ggacgttctg
     9061 ccgcaagaaa tggataaact gcgtgagatg atgctcgaat tgatcaacca gccggaacac
     9121 tttaagcaat ggtttggcga gtttatatcc cagtcacgtc atgaactgga tatcgcgccg
     9181 ccggaaccgc cttatcagcc agatgaaatc tacgatgcgc tgaaacaagg tgatgtgctg
     9241 gtgcgcctgg gtggtctgcg cgtattgcgc attggcgacg acgtgtatgc caatggtgag
     9301 aagatcgatt ccccgcaccg tccggcactg gatgcactcg ccagcaacat tgcgctgacc
     9361 gcggagaatt ttggcgatgc gctggaagat ccgtcattcc tcgcgatgct cgcggcgctg
     9421 gtcaatagcg gatactggtt cttcgaaggg taagtttgga tttaagccgg atgcggcatc
     9481 acatgccgca tcccttttcg cttacttccg ttgcgccgtt aactcggcaa tacggacgat
     9541 cacctgcacc gctttttcca taccttccag agtcacaaac tcatgcttac catgatagtt
     9601 gtaaccgcca gtgaacaggt tcgggcacgg taatcccata aacgacaact gcgcgccgtc
     9661 ggtaccgccg cggatcggtt tcagttccgg ttcaatatcg cagtcacgca tcgcctgctg
     9721 ggcgatatcg agaatatgcg gatgctcaac cactttctcg cgcatattgt agtaactgtc
     9781 ttcaatcacc agttcaatgt agcaatcagg atgtaaccct ttgcccactt ttttggcgat
     9841 ctccatcatt ttacgtttac gcgcttcaaa ctgtttacgg tcgaaatcac ggatgatgta
     9901 gtgcatatcg gctcggtcaa cggtgccttt catgctcgcc aggtgataga aaccttcata
     9961 gccttctgtc atttccgggc tttcatccgc cggaacttcc gcatgaatac gtgccgccag
    10021 cgacagcgca tttaccatca ctcctttcgc cgtgcccgga tgaacattgt taccgacaat
    10081 tttgatattg accgatgcgg cgttgaagtt ttcaaactcc agttcgccta cgccaccacc
    10141 gtcaacagtg taagcccagc gggcatcgaa ggcatcaaca tcaaaatgtt tcgccccttt
    10201 gcccacttct tcatccgggg taaaggcgac gcgaatatca ccatgcggaa tgtttttctg
    10261 ttgcaatacc gccagcgcgg tcatgatttc tgcaataccg gctttgtcat cggcacctaa
    10321 caaggtttta ccatcggtgg taatcaacgt ctgacccagt agctgatgca gcaccgggaa
    10381 cataaccggt gataaaactt catcgccgat acccagcgca atatcgccac cgcgatagct
    10441 ttcaacaatt tgcggattaa catttttgcc gctgcaatcc ggtgaggtat ccacatgaga
    10501 aataaagcca atcgccggga tatcgccagg gacgttagcc ggtaacgtcg ccatcaaagt
    10561 gcccttctca cttaaggtca cattgataag ccccatttct tcgagctgct ctttcagcag
    10621 atgcaataac ttccattggc cttccgtgct gggaacctgt ctcacccctg cttttgattg
    10681 ggtatccaga gacacgtagt tcaaaaatcg ctcaagtagt ttatccatgt agtcaccctc
    10741 actttttgtg acaacattat taagaagcaa gaaaagacaa atattgcgtc aggtcacttt
    10801 tacccctgca agcgggaata tttatcagca ttaccttaat gaatataaag ctaagccagt
    10861 aattcacaac aaagattggc gattcaagcg gtttgccgta gaattaccgc cctcattaag
    10921 agtcaccaag gtggttaacc acaaaccccg catcggtaag ccatccgttg cgtttacatg
    10981 ggacagagta aaaaattgaa taaacaaccg agttcgcttt caccgctggt gcaattggcg
    11041 ggaattcgca aatgctttga tggtaaagag gtcattcccc agctggatct gactatcaac
    11101 aatggcgagt tcctcacgct gcttggccct tctggctgcg gtaaaacaac cgttcttcgc
    11161 ctgattgcag gtctggaaac tgttgattcc ggacgcatca tgctggataa cgaggacatc
    11221 acccacgttc cggcggaaaa ccgctatgtg aacactgttt tccaaagcta cgcacttttc
    11281 ccccacatga ccgtgttcga aaatgtggcc tttgggttgc gcatgcaaaa aacccccgct
    11341 gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg tgcaactgga aaccttcgct
    11401 caacgtaaac cgcatcagct ctctggtggt caacagcaac gcgtcgccat tgctcgcgcg
    11461 gtggttaaca agcctcgtct gttgttgctg gatgagtcgc tctcagcgct ggattacaaa
    11521 ctgcgtaagc aaatgcagaa cgagctgaaa gcgttacagc gtaagcttgg tattaccttc
    11581 gtctttgtca ctcacgatca ggaagaagcg ctcaccatgt cagacaggat tgtggtgatg
    11641 cgcgagggtc gtattgagca agatggtaca ccgcgcgaaa tttacgaaga gccgaaaaac
    11701 ctgtttgttg ccggcttcat tggcgaaatc aatatgttta acgccactgt catcgaacgt
    11761 ctagacgagc agcgcgtacg cgccaacgtt gaaggccgcg aatgtaatat ctacgttaac
    11821 ttcgccgttg aaccggggca aaaactgcat gttctgctgc gcccggaaga cttacgtgtt
    11881 gaagagatta acgacgacaa ccacgctgaa gggctgattg gttacgttcg tgagcgtaac
    11941 tacaaaggca tgacgctgga gtcggttgtt gaactggaaa atggcaagat ggtgatggtc
    12001 agcgaattct tcaatgaaga cgaccctgac tttgaccact ctctcgacca aaaaatggcc
    12061 attaattggg tagaaagctg ggaggtcgta ctggctgatg aagaacacaa gtaagttcca
    12121 gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg tttgtctttc tgcccaacct
    12181 gatgatcatt ggcaccagct ttttgacccg cgacgacgcc agtttcgtca aaatggtctt
    12241 tacgctggat aactacacgc gtctgctcga tccgctctat tttgaagtgc tattgcactc
    12301 gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg ctgggctacc cgtttgcctg
    12361 gtttctggcg aagctaccac acaaggtgcg tccgctgctg ctgtttctgc tgattgttcc
    12421 cttctggacc aactcactga ttcgcatcta cgggctgaaa attttcctca gcaccaaagg
    12481 ctatctcaac gagtttttgc tctggctggg cgttatcgac acaccaattc gtatcatgtt
    12541 cacgcccagt gcggtgatta tcggtctggt ttacattctg ctaccgttta tggtgatgcc
    12601 gctgtactcc agtatcgaaa aactggataa gccgttgctt gaggcggcgc gcgatctcgg
    12661 tgccagcaag ttacagactt ttatccgtat cattattcca ctgacgatgc cgggaattat
    12721 tgccggatgt ctgctggtga tgctgccagc gatgggcctg ttctatgtat ccgacctgat
    12781 gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag gtccagttcc tcaatattcg
    12841 tgactggccg tttggtgcag ccaccagcat tacgctgact atcgtaatgg gcctgatgtt
    12901 gctggtttac tggcgcgctt ctcgtctgct gaataaaaag gtggaactcg aatgatcggt
    12961 cgactgcttc gcggcggttt tatgaccgct atctacgcgt acctgtatat cccaatcatt
    13021 attttgattg tgaactcctt taacagttcg cgctttggca tcaactggca gggttttacc
    13081 accaaatggt atagcctgct gatgaacaac gacagcctgt tacaggcagc gcagcattca
    13141 ctaacaatgg cggtgttttc ggcgacattt gctacgctta tcggttcact gacggcagtt
    13201 gcgctgtacc gttatcgctt tcgtggtaag ccgttcgtta gcggaatgct gtttgtggtg
    13261 atgatgtcac cagatatcgt gatggcgatt tctctgctgg tgctgtttat gctgctgggt
    13321 attcagcttg gcttctggtc gctgctgttc tcgcatatca ccttctgcct gccgtttgtg
    13381 gtggtgacgg tgtattcgcg cctgaaaggt tttgacgtgc ggatgctgga agcggcgaaa
    13441 gatctcggtg ccagtgaatt taccattctg cgaaaaatca ttctgccact ggcaatgcct
    13501 gcggtggcgg cgggctgggt gttaagcttt accctgtcga tggacgatgt ggtagtttct
    13561 tcgttcgtca ccggaccaag ttatgaaatt ctgccgttaa aaatttattc gatggtcaaa
    13621 gtcggcgtat cgccggaagt taacgcgctg gcaaccatat tactggtgct gtcgctggtg
    13681 atggttattg ccagccagct tattgctcgt gataaaacga aaggtaacac aggggacgtt
    13741 aaatgaaaaa atggtcacgc cacctgctcg cggcgggtgc tctggcactg ggcatgagcg
    13801 ccgctcacgc cgatgacaac aacacgctgt atttctacaa ctggaccgag tacgtgccgc
    13861 caggactgct tgaacagttc accaaagaaa ccggtattaa ggttatctat tcgacttacg
    13921 agtcgaacga aaccatgtat gccaagctga aaacgtacaa agacggtgcc tatgacctgg
    13981 tggttccttc aacctattac gtcgataaaa tgcgtaaaga agggatgatc cagaagatcg
    14041 acaagtcgaa gttaacgaac ttcagcaatc tcgatccaga catgctcaat aagccgtttg
    14101 acccgaataa cgattactcc attccgtata tctggggtgc gacggcgatt ggtgttaacg
    14161 gtgatgcggt ggatccgaaa tctgtcacca gctgggccga tctgtggaag cctgagtaca
    14221 aaggcagcct gctgttgacc gacgatgccc gtgaagtgtt ccagatggcg ctgcgtaagc
    14281 tgggctactc cggtaacacc accgatccga aagagattga agctgcatat aacgagctga
    14341 aaaaactgat gccaaacgtg gcagccttta actccgataa cccggctaac ccgtacatgg
    14401 aaggcgaagt taacctcggc atgatctgga acggttctgc tttcgttgca cgccaggcgg
    14461 gtacgccaat tgacgtggta tggccgaaag aaggcggcat tttctggatg gacagcctgg
    14521 cgatcccggc aaatgccaaa aacaaagaag gcgcgctgaa attgatcaac ttcctgctgc
    14581 gcccggatgt ggcaaaacag gttgccgaaa ctatcggtta tccaacgcca aaccttgcgg
    14641 cgcgtaagct gttaagcccg gaagtggcga acgataaaac gctctacccg gatgctgaaa
    14701 ccattaaaaa tggtgaatgg cagaatgacg ttggcgcagc cagcagcatt tatgaagagt
    14761 attatcagaa gctgaaagca ggacgttaat tcagcaagcg ggcggtggca ttaccgcccg
    14821 ttttttattt agcaaggatg tggctaatga gtcaggacag taaagtgtta tcccgcgctt
    14881 ttctcggtat cggtctgata ttgctcgtta tttctgcaat catcttttat taccatttta
    14941 cttttaccaa aagcgcggtt catacagaag gaatcattgt tgatgccgtt tggtacaaca
    15001 atcactcaaa cgacgtcgat gataatggtt cctggtatcc agtggttgcc tttcgcccta
    15061 cgccagatta cacgctaatt ttcaattcga atatcggtag tgatttttat gaagatagcg
    15121 aaggtgacag agtcaatgtc tattatccgc cagggcatcc agaacaagcc gaaataaaca
    15181 atccctgggt taattttttt aaatggggct ttgttggcat agcaggcatt attttcggta
    15241 gtgtcggtct gataatcacg ctcccctcca ccaaaaaatc tcgcaggaaa cggaagtccc
    15301 gcccatgaaa aaattcatta tactgctcag tttactgata ctcctgcctc tggtagcaac
    15361 cagtaaacca ttgattccaa taatgaagac actttttacg gatgttaccg gtactgtacc
    15421 ggatgctgaa gagatcgcac gtaaagccga gctttttcgc caacaaacgg gaattgcacc
    15481 gtttattgtg attttacccg acattaataa tgaagccagt ctcagacaaa atggtaaagc
    15541 gatgctggcg catgcgtcat cttcattgag taatgtaaaa ggaagtgttc tgttactatt
    15601 tactacccgc gaaccacggt taattatgat caccaacggc caggttgaaa gtggtctgga
    15661 cgataaacat ctcggccttc tgatagaaaa tcacacgctg gcttatttaa atgcagatct
    15721 ctggtatcag ggaatcaata atgcattggc tgttctacaa gcacagatat taaaacaacc
    15781 gacgccgcca ctgacgtatt atccgcatcc aggacaacag catgaaaatg ctccccccgg
    15841 cagcaccaac acattaggtt ttatcgcctg ggctgcgacg tttatcttat tttcctggat
    15901 cttttgctac acgactcgtt ttatttatgc gttaaaattt gcagtggcga tgactattgc
    15961 caatatgggt tatcaggctt tatgtttgta tatcgataat agctttgcga ttaccagaat
    16021 atcgccactc tgggcagggc taattggtgt ctgtacgttt attgccgcat tgttattgac
    16081 gagtaaacga taaaagaggc cggaaaagtg cgcgtgtatt atgccggatg gggcgcgaac
    16141 gccttatccg gcccacaaaa cccgcaaatt caattaattg cgtccccttg taggcctgat
    16201 aagcgtagcg catcaggcaa tgcttcctgc tttataatcc cttcagcaac ttttcaacaa
    16261 actcaggcac cacctggctt gccgggccgt aatatttctc ggcaaattca ttaccgacct
    16321 gacttggttc gagattcagt tccacggtgt gcgcgccatg cagtttcgct tcgtgaacaa
    16381 acccagccgc cggataaaca tgcccggaag taccaatggc aatgaaaata tcagccatcg
    16441 acaacgccat ataaatttca tccatgccga gtggcatttc gccaaaccaa acgacgtgcg
    16501 ggcgcaaggg tgccgggaac tggcaacaat ggcatttatc ttctggcgta acgtctccgg
    16561 tccagtcgag aacctgacca ctttgcgaac aacgaacttt cagcaattcc ccatgcatat
    16621 gaatgacatt ggtattacct gcgcgttcat gcaggttgtc tatattctgc gttaccagca
    16681 aaaagcgatc gccgagggcg tcttgcagtt tagccagcgc aagatgcgcg gcgttaggct
    16741 gaatttctgg ctgctgcagc tgtcgacgac gggcgttata aaacgcttgc accagttcag
    16801 gatcgcgatc gaaaccttcc ggcgttgcca catcttcaac ccgatgttct tcccacaggc
    16861 catctgcggc gcgaaaggta cgaatacctg attccgcaga aatccctgcc cctgtcagta
    16921 cgagtactct tggtttttcc attgcttccg gcaccacttt atctctgaaa aaaatacgct
    16981 gacgcaaacg ctcgcgcagg cggcgtttat ttttacgaaa acgacttaac cgatgacccc
    17041 gacgcgacag catagcaacc tctgttgttt aatcggttag atgtaggaag gccgcaccac
    17101 gcattcctcc cgcatcaccg tggcgcgcgc gttcaatgcg cggaacacga gctacaggta
    17161 agagatgacg aggcagcctg tccgccagtt gcgttgtgat tgccgggaaa ttcgataagc
    17221 caccaccaat gacgaccagg tcagggtcaa caatggtcag gatatttccc agacaaaccg
    17281 ctaataaatc cagataacgc tcaacgtgcg cccttgcctg ctcatcgcct tgatcataaa
    17341 gcgcaataat ttcgggagcc tgcaacggtt gatgataata gtgttgatac agccacgcaa
    17401 aaccgcgacc agacagataa ttttcaatgc agccatgctg accacagccg cagcggcgta
    17461 acgggaaatc caaccccatc atggttaacg catcaaccgg cagacgcata tggccaaact
    17521 cgccggtaat gtagctttta ccggtaatcg gtttgccgtt gaaaatcagc ccgccgccaa
    17581 cgccggtgcc gagaatcaac cccatcacca gtggatattg agtaaattca tcatcccagg
    17641 cttctgaaag ggcaaaacag ttggcatcgt tatcaaggcg tacatcgcga tcaagacgtg
    17701 cgctcaggtc ggcacgcagc ggtttaccgc tggcagcggg gacattggcg gcatacagcg
    17761 taccatcttc tgtttccggc attcccggaa taccgatgcc gacagaacct ttacagccaa
    17821 aacgttggtc agcttcagct accagctcac acactgcatc taaaaatgcg tcatagctgt
    17881 cacgcggtgt cggcacccgc ttttcccact gcaactgccg accgctatca aacacgccaa
    17941 gcgcaatttt tgttccacca atatcaaacc cgtaatacat tgccgctcct tgttttaatg
    18001 tactgccttt actggccgct aaggactcgc gcagggtcaa tattactggc gcgccgcgcc
    18061 ggataccaac ttgccaaaag actcagcaac aatgctgtga ccagtacgta gaagacgtcc
    18121 agccagtgca attccgatgg caggaaatca ataaaataga tatcgctgga gaggaactga
    18181 tgaccgatca acttttcaat ccactcaata atcggggtaa gttgcagtga aaccaccaca
    18241 ccgataatca ccccacacag gctaccgaac agccctgcca gcaatccata ccagacaaag
    18301 atggcgcgaa ttaaaccatc tttcgccccc agcgttctta atactgcgat atcgccactc
    18361 ttgtctttca ccgccatcac taaggtggag acgatgttga aacaggccac gccaatcacc
    18421 agtaccatcg ccagatacat aatggcgcgg atcatctgga tatcgcgata catatagccg
    18481 taagtaccaa tccaactttt aatataaaca tagctgttgg tcacttcacc cgcatcgcgt
    18541 accagcttat tggcgttgaa aacatccgtc attttaaggg caatacctga cacgctggaa
    18601 cccatatcaa gatattgttg ggcatccgcc agcgggatca tggcaaaact gtgatcgagt
    18661 tgaccactca actgcaaaat accggcaatg tgcaaacgca cacgttttgg ctgcatcagt
    18721 ttatgctcgg gattcgagtt ggggatcatg atcgacaccc aatcgccctg cttcactttc
    18781 agcgcatccg ccacgccttt gccgatgata atttgctgtt cgcccgcttt aaaattgcgc
    18841 caggcatccc cctgaacaaa cgagggtaat gcgctcagac gctgttcctg ttgcgggtta
    18901 acgcccttca cctgaattgc gcgcagattg gcaccacttt ctaccagtcc ggtgaaattg
    18961 atatacggcg cagcagcggc aatacccggc actttttgca cgttatccag tgcttcctgc
    19021 cagttagtcc atggctgatt taccgcctca atttcaccat gcggcaccac cgccagaata
    19081 cggttattca gttcgcgttc aaagccgttc atcgcgctta agccgacgat caataccgcc
    19141 acgccgaggg caatgccaat ggtagaaata acggagatca gcgacaccat gccgccgcgc
    19201 cgccgtccgc gactaaaacg caggccaatc aataacgata aaggcatcgc cattactccg
    19261 cccccatcag gctcagttcc gccgtcagac gtccatcacg catttccaat tggcggctca
    19321 tacgtttcgc cagttgcagg tcgtgagtaa ccaccaggaa ggcggtgccc tgcaagcgat
    19381 tcaattcccc aagcaactgg aagatgctgt cggcgttacg cgcatcgagg ttaccggtag
    19441 gctcatccgc aagtaccaga cgcggattat tgaccagcgc acgggcaatc gccacacgct
    19501 ggcgttcgcc gccagaaagt tcagatgggc ggtgattcgc acgatgctcc agccccaccg
    19561 cttttaacat ctccagcgca cggctgttga tttcagcggg ctttttcttg ccaatcagca
    19621 gtggcatagc cacgttttcc agggcagtaa aatccggcag caggtggtga aactgataaa
    19681 taaagcccag cttctggttg cgcagttctg ctttcgccgc cgaagacagt ttgctcatcg
    19741 gttgaccgtt aaagatcaca tcgccggagg ttggtgtatc cagcccgccc agcaggtgca
    19801 gcaaggtact tttaccggaa ccagagctac cgacgatcgc catcatttca ccttcgccca
    19861 cgctgaaact gacattgtgc agtacatcgg tttgcacact gccttcctga tagcgtttgc
    19921 acaggttgtc gcattgcaac aggatcttat tcataacgta aagcctcagc gggttgtgtg
    19981 gcggcagcgc gccatgaagg gtaaagcgta gacagcagcg cgatagccat cgccaccagc
    20041 gcaataacaa tgacctgtaa aggttcgata gccaccggca gcgccgcgcc atcaagcagg
    20101 acgccgatta tcggcatcag attatttaac tggctggcaa gcagtgcgcc aagcgccgca
    20161 ccgaggatcg caccgataat cccggcgctg gccccttgca ccataaagac catcatgatt
    20221 tgtcgcggag ttaagccttg cgtttgcagg atcgcgactt cgccctgctt ctccattacc
    20281 atcagcccta gtgaggtaat aatgttaaac gccgcaacgg cgacaatcag gctcagcagc
    20341 aagcccatca tatttttttc catgcgtacg gcctggaaca gctcgccttt acggtcacgc
    20401 cagtcctgcc atttgctgcc ttcaggcagt ttttgctgac ttaaagagtc aactttcagc
    20461 ggctcatcca gccacaaacg ccagccggta atattgcctg ccggataacg catcagacgc
    20521 gaggcatcct caatattcac cagcatttca tagccatcga cttcactgtt ggcggcgaaa
    20581 gtgccaatca cattgaacag gcgctggctt ggaatacgcc ccatcggcgt gaactggctg
    20641 gcagatggta ccatcacgcg gatttgatca ccgcgattaa cgcctagctg tgaggcaagt
    20701 tgttcgccga ggatgacatt atatttcccc ggctcgaggt cagtttgttt cacattgacc
    20761 agatacggtg taagtggatc tttttgcgcc gggtcgatac cgagcatcac cccgaccgcc
    20821 acgctgcgcg cgctttgcag taccacatca ccggtagtaa taggtgcgac gcgattaacg
    20881 ccgtccagtt tgactgccgt ttccgggagt tgctgcgggt taagagagcc atgctcagaa
    20941 gagagaattg cctgtggcat caggccaagg atgttgtttt gcagctcgcg ctcaaagccg
    21001 ttcatcactg acaatactgt gaccagcgcc atcaccccga gggtaatgcc gatggtagaa
    21061 agccaggaga cgaaacgacc gaagcgatcc gctgcacgcc cacgcatgta acgcaggcca
    21121 ataaatagag cgacaggttg gtacatgaaa tccgtctggt tgctgtagca aagacacgag
    21181 tatataagcg aaagcaagat gggtaaacgt ctgaactgta agtgatcgtc agggctaatc
    21241 tgaaaagccc ccgtaaatca atcggctaaa ttgccatgtt ttatcatcaa tcttgccgcc
    21301 tgatcttaat ctgaaaaatc atccaataca gacagatacc cattacacct cgggtcataa
    21361 cccgcaggat caaccgcatg taaattaaaa aagagcgtgt tgtcctgatg aaacaaaaag
    21421 agctatggat taaccagatc aaagggttat gtatttgtct ggtggtgatt tatcactcgg
    21481 tcattacctt ttatccgcat ctgaccactt ttcagcatcc gttatcggaa gtcctgagca
    21541 aatgctggat ctatttcaat ctttaccttg ccccctttcg tatgccggtt tttttcttta
    21601 tatccggcta tttgattcgc cgctatatcg acagcgtacc gtggggaaat tgtctcgata
    21661 aacggatctg gaacatcttc tgggtgctgg cgctttgggg cgtggtgcag tggctggcgt
    21721 taagtgcgct gaaccagtgg ctggcacctg agcgcgattt aagcaatgcc tccaatgccg
    21781 cttatgccga ttccaccggt gagttcctgc acgggatgat caccgccagc accagcttgt
    21841 ggtatctgta tgcgttaatt gtctatttcg tggtatgtaa aatttttagc cgtctggcgc
    21901 tgccactatt cgccttgttt gtactgctga gtgtggcggt taatttcgtt cccacgccgt
    21961 ggtggggaat gaacagtgtg atccgcaatt tgctttatta cagccttggc gcatggtttg
    22021 gcgcaacaat aatgacctgt gttaaagagg tgccgttgcg ccgccatctg ctgatggctt
    22081 ctttgcttac cgttctggcg gtcggtgcct ggttgtttac tatctcgctg ctgttgtcgc
    22141 tggtatcgat tgtggtgatc atgaagctgt tttatcaata cgagcaacga ttcggtatgc
    22201 gctccaccag cctgctgaat gtgattggct ccaacaccat tgctatctac accacccatc
    22261 gcattctggt tgaaatattc agcttaactc tgcttgcgca aatgaacgta gcacgctggt
    22321 cgccgcaagt cgaactgaca ctcctgctgg tttatccctt tgtcagtttg ttcatctgta
    22381 ctgttgcggg cttgctggta agaaaacttt cacagcgtgc attcagcgat ctgttgttct
    22441 ccccgccttc actgcccgct gccgtcagtt actctcgtta aaccatcgcc ccagccgggg
    22501 cgattcaatt tgctaaacca tgtcgattac ggataatgaa cagcattgct tatcagagat
    22561 atgcccccat atgttgaggc atatcctaac gagaatctga caaccgttat gcctgaacaa
    22621 tatcgttata cgctgcccgt caaagcgggt gagcagcgtc tgctgggcga gttaaccggc
    22681 gcagcctgtg caacgctggt agcggaaatt gccgaacgtc acgccggtcc ggtggtactc
    22741 attgcaccag atatgcaaaa tgctctgcgt ttgcatgatg aaatcagcca gttcaccgat
    22801 cagatggtga tgaatctggc ggactgggaa actcttccct acgacagttt ttcgcctcat
    22861 caggacatta tctcctcgcg cctttccacc ctttaccagc taccgacgat gcagcgtggc
    22921 gtactgattg ttccggtgaa tacgcttatg cagcgcgttt gcccacacag ttttctccac
    22981 ggtcatgcgc tggtgatgaa aaaaggtcag cgcctgtcac gagatgcatt acgaacccaa
    23041 ctggacagcg ccggttatcg ccatgttgac caggtgatgg agcacggcga atacgccacg
    23101 cgcggcgcgt tgctggatct cttcccgatg gggagtgagc tgccttatcg tcttgatttc
    23161 tttgatgatg aaatcgacag cctgcgggtg tttgacgtcg acagccagcg cacgctggag
    23221 gaagtagaag cgatcaatct gctgcccgcg cacgaatttc cgaccgataa agcggcaatt
    23281 gaactgttcc gcagccagtg gcgcgatacc ttcgaagtga agcgcgatcc ggaacatatt
    23341 taccagcaag tgagtaaagg cacattacct gccgggatcg agtactggca gccattgttc
    23401 ttcagcgaac cactgccgcc gctgttcagt tatttccctg ccaatacctt gctggtgaat
    23461 actggcgatc tggaaaccag tgccgaacgt ttccaggctg acacgctggc gcgttttgag
    23521 aatcgcggcg tcgatccgat gcgcccgctg ttgccaccac aatcgctctg gctgcgggtg
    23581 gacgagctct tctcagagct gaaaaactgg ccccgggtgc agctaaaaac tgaacattta
    23641 ccgacaaaag ccgcgaatgc caatttaggt ttccagaaac tgccagacct ggccgttcag
    23701 gcacaacaaa aagcgccgct ggatgcgctg cgtatgttcc tcgagacttt cgacggtccg
    23761 gtggtgttct cggtagaaag tgaaggtcgc cgtgaagcgc tgggtgaact gctcgcacga
    23821 attaaaattg ctccgcaacg cattatgcgt cttgatgaag ccagcgaccg tgggcgttat
    23881 ctgatgattg gcgctgccga acatggtttt gtcgataagg tgcgtaatct ggcgctgatc
    23941 tgcgaaagcg atctgctcgg tgaacgtgtt gcccgccgtc gtcaggattc tcgccgcacc
    24001 atcaaccccg atacactgat ccgtaacctt gcggagctgc atattggtca gccggtggtc
    24061 catctggagc acggtgtcgg gcgctatgcc ggaatgacca cgctcgaagc gggcggcatt
    24121 actggcgagt atttgatgct cacctatgcc aacgacgcca aactgtatgt tccggtgtcg
    24181 tcactgcatc tgattagccg ttacgcaggt ggcgcggaag aaaacgcccc gctgcataaa
    24241 cttggcggcg atgcgtggtc acgcgcgcgg cagaaagcgg cggaaaaagt gcgtgatgtg
    24301 gcggcggaat tgctggatat ctacgcgcaa cgcgccgcca aagagggctt cgcgtttaaa
    24361 cacgatcgtg agcagtatca gttgttctgc gacagcttcc cgtttgaaac cacgccggat
    24421 caggcgcagg ccattaatgc ggtacttagc gacatgtgtc agccgctggc aatggatcgt
    24481 ctggtgtgcg gcgatgttgg ctttggtaaa acagaagtgg cgatgcgcgc cgctttcctg
    24541 gcagtagata accacaagca ggtggcggtg ttggtgccta ccacccttct cgcgcagcag
    24601 cattacgaca acttccgcga ccgtttcgcc aactggccgg tacgtatcga aatgatctcc
    24661 cgtttccgca gcgccaaaga gcagacgcaa atccttgcgg aagtggcgga agggaaaatc
    24721 gatattctga tcggtacgca caaactgctg caaagtgacg tcaagtttaa agatttaggc
    24781 ctgctgattg tcgatgaaga acaccgcttc ggggtgcgtc ataaagagcg tattaaagcg
    24841 atgcgcgcga acgtggatat tctgacgctt actgcaacgc cgatcccacg tacgctgaat
    24901 atggcaatga gcggaatgcg tgacctgtcg attatcgcca cgccgcccgc ccgtcgtctg
    24961 gcagttaaaa cctttgtccg tgagtatgac agcctggtgg tccgggaggc gatcctgcgt
    25021 gaaattttgc gcggaggaca ggtttattat ctctacaatg atgtggaaaa cattcagaaa
    25081 gccgccgaac ggctggcaga actggtgcca gaagcgcgga tcgccatcgg tcacgggcag
    25141 atgcgcgagc gcgaactgga acgggtgatg aatgatttcc atcatcaacg tttcaacgtg
    25201 ctggtttgta ccaccattat cgaaaccggg atcgacatcc cgacagccaa caccattatc
    25261 attgaacgtg cggatcactt cggtctggcg cagctgcacc agttacgcgg tcgcgtcgga
    25321 cgttcgcatc atcaggcata tgcatggttg ctgacgccgc atccaaaagc gatgactacc
    25381 gatgcacaaa aacgtcttga agcgattgcc tcgctggaag atctcggtgc aggctttgcg
    25441 ctggcaacgc acgatctgga gattcgcggc gcgggtgaac tgcttggcga agaacaaagc
    25501 ggctcaatgg aaaccatcgg tttctcgctg tatatggagt tgctggaaaa cgccgtcgat
    25561 gcactgaaag ccggacgcga gccgtcgctg gaagatctca ccagccagca aacagaagtc
    25621 gagctgcgga tgccgtcgct attgccagat gatttcattc ctgacgtgaa tacgcgtttg
    25681 tcgttctata aacgtattgc cagcgccaaa acggaaaacg aactggaaga gatcaaagtc
    25741 gagcttatcg atcgcttcgg cctgctgccg gatccggcgc gtatcctgct ggatgttgcc
    25801 cgtctgcgcc agcaagcgca gaaactgggg atcaggaagc tggaaggtaa tgagaaaggc
    25861 ggggtgatcg aatttgccga gaagaatcac gttaatccgg cctggttgat tggtttgctg
    25921 caaaaacagc cgcagcatta ccgccttgat ggtccgacgc gcctgaaatt tattcaggat
    25981 ttgagtgagc ggaaaacgcg tatcgaatgg gtacgccagt ttatgcgtga actggaagag
    26041 aacgcgatcg cttaatggta aatcgggggc gtttctgcgc ccccatctgc aacatttaca
    26101 aattctttgc acttccctgc actatccgac actgtcacca tccataattc aggcttatct
    26161 gtttattaca ataaccttat atttattatg gatttttggt gatgatcaaa acgcgttttt
    26221 ctcgctggct aacgtttttt acgttcgccg ctgccgtggc gctggcgcta ccggcaaaag
    26281 ccaacacctg gccgctgccg ccagcgggca gtcgtctggt tggcgaaaac aaatttcatg
    26341 tggtggaaaa tgacggtggt tctctggaag ccatcgccaa aaaatataac gtcggctttc
    26401 tcgctctgtt acaggctaac cccggcgttg atccttacgt accgcgcgcg ggcagcgtgt
    26461 taacgatccc gttgcaaacc ctacttccag acgcgccgcg cgaaggcatt gtgatcaaca
    26521 ttgcggagct gcgtctctat tactacccgc cgggtaaaaa ttcggtaacc gtgtatccaa
    26581 taggtattgg tcagttaggt ggtgacacgc tgacaccgac aatggtgacc accgtttcag
    26641 acaaacgtgc aaacccaacc tggacgccaa cggcaaacat ccgcgcccgt tataaagcac
    26701 agggaattga gttgcctgcg gtagtgccgg ctggaccgga taacccaatg ggccatcatg
    26761 cgattcgtct ggcggcctat ggcggcgttt atttgcttca cggtacgaac gccgatttcg
    26821 gcattggcat gcgggtaagt tctggctgta ttcgtctgcg ggatgacgat atcaaaacac
    26881 tctttagcca ggtcacccca ggcaccaaag tgaatatcat caacactccg ataaaagtct
    26941 ctgccgaacc aaacggtgcg cgtctggttg aagtacatca gccgctgtca gagaagattg
    27001 atgacgatcc gcagctgctg ccaattacgc tgaatagcgc aatgcaatca tttaaagatg
    27061 cagcacaaac tgacgcagaa gtgatgcaac atgtgatgga tgtccgttcc gggatgccgg
    27121 tggatgtccg ccgtcatcaa gtgagcccac aaactctgta aatcctggat aaaaaaaatc
    27181 cccgcgacac ggcatgtcgc ggggaaaaaa tcagcagtag caggcattaa tgagggttaa
    27241 tgcttattta taaattactg ctgttccatg gagggtattc ggaccggtta cagaagtaat
    27301 acggaaagat tttgcgccca tctcatccgc tttttgcgcc agctgctctt ccagcgatcc
    27361 cagatttgtc cccgcgttag cactgattgt accgactttt tgttggcctt ctggcgttga
    27421 ctgaacttcg acagccgcaa agctggcaaa tgacatggag cttaaaatcg ccgcagcgat
    27481 gagggttttt acgtttttca taatagtggc cttatgcaga tgaatgacgt cgcaagcaat
    27541 tacttaacga tcgataact
//
LOCUS       DAJOWB010000055        27540 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJOWB010000055 DAJOWB010000000
VERSION     DAJOWB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.055"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            101..832
                     /gene="artJ"
                     /locus_tag="OHO85_002517"
     CDS             101..832
                     /gene="artJ"
                     /locus_tag="OHO85_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCQ2671877.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(1007..2134)
                     /gene="rlmC"
                     /locus_tag="OHO85_002518"
     CDS             complement(1007..2134)
                     /gene="rlmC"
                     /locus_tag="OHO85_002518"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCQ2671878.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRFETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(2175..2663)
                     /locus_tag="OHO85_002519"
     CDS             complement(2175..2663)
                     /locus_tag="OHO85_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCQ2671879.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(2723..3568)
                     /gene="potI"
                     /locus_tag="OHO85_002520"
     CDS             complement(2723..3568)
                     /gene="potI"
                     /locus_tag="OHO85_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCQ2671880.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(3565..4518)
                     /gene="potH"
                     /locus_tag="OHO85_002521"
     CDS             complement(3565..4518)
                     /gene="potH"
                     /locus_tag="OHO85_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCQ2671881.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLSEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(4528..5661)
                     /gene="potG"
                     /locus_tag="OHO85_002522"
     CDS             complement(4528..5661)
                     /gene="potG"
                     /locus_tag="OHO85_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCQ2671882.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5756..6868)
                     /gene="potF"
                     /locus_tag="OHO85_002523"
     CDS             complement(5756..6868)
                     /gene="potF"
                     /locus_tag="OHO85_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCQ2671883.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHVYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(7219..7695)
                     /locus_tag="OHO85_002524"
     CDS             complement(7219..7695)
                     /locus_tag="OHO85_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCQ2671884.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7783..8685)
                     /gene="rimK"
                     /locus_tag="OHO85_002525"
     CDS             complement(7783..8685)
                     /gene="rimK"
                     /locus_tag="OHO85_002525"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCQ2671885.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(8746..9468)
                     /gene="nfsA"
                     /locus_tag="OHO85_002526"
     CDS             complement(8746..9468)
                     /gene="nfsA"
                     /locus_tag="OHO85_002526"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCQ2671886.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(9452..9739)
                     /locus_tag="OHO85_002527"
     CDS             complement(9452..9739)
                     /locus_tag="OHO85_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCQ2671887.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9899..10156
                     /gene="grxA"
                     /locus_tag="OHO85_002528"
     CDS             9899..10156
                     /gene="grxA"
                     /locus_tag="OHO85_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCQ2671888.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(10186..10563)
                     /locus_tag="OHO85_002529"
     CDS             complement(10186..10563)
                     /locus_tag="OHO85_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCQ2671889.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            10833..12518
                     /locus_tag="OHO85_002530"
     CDS             10833..12518
                     /locus_tag="OHO85_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCQ2671890.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(12693..13229)
                     /gene="rcdA"
                     /locus_tag="OHO85_002531"
     CDS             complement(12693..13229)
                     /gene="rcdA"
                     /locus_tag="OHO85_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCQ2671891.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            13313..14521
                     /locus_tag="OHO85_002532"
     CDS             13313..14521
                     /locus_tag="OHO85_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2671892.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHISLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14521..15336
                     /gene="ybjI"
                     /locus_tag="OHO85_002533"
     CDS             14521..15336
                     /gene="ybjI"
                     /locus_tag="OHO85_002533"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCQ2671893.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            15422..15706
                     /locus_tag="OHO85_002534"
     CDS             15422..15706
                     /locus_tag="OHO85_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2671894.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(15747..16979)
                     /gene="mdfA"
                     /locus_tag="OHO85_002535"
     CDS             complement(15747..16979)
                     /gene="mdfA"
                     /locus_tag="OHO85_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCQ2671895.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            17264..17860
                     /gene="ybjG"
                     /locus_tag="OHO85_002536"
     CDS             17264..17860
                     /gene="ybjG"
                     /locus_tag="OHO85_002536"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCQ2671896.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGFTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            17918..18676
                     /gene="deoR"
                     /locus_tag="OHO85_002537"
     CDS             17918..18676
                     /gene="deoR"
                     /locus_tag="OHO85_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCQ2671897.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(18723..19925)
                     /gene="dacC"
                     /locus_tag="OHO85_002538"
     CDS             complement(18723..19925)
                     /gene="dacC"
                     /locus_tag="OHO85_002538"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCQ2671898.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            20172..20798
                     /gene="gstB"
                     /locus_tag="OHO85_002539"
     CDS             20172..20798
                     /gene="gstB"
                     /locus_tag="OHO85_002539"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCQ2671899.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(20795..21910)
                     /gene="yliI"
                     /locus_tag="OHO85_002540"
     CDS             complement(20795..21910)
                     /gene="yliI"
                     /locus_tag="OHO85_002540"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCQ2671900.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(22021..22404)
                     /gene="bssR"
                     /locus_tag="OHO85_002541"
     CDS             complement(22021..22404)
                     /gene="bssR"
                     /locus_tag="OHO85_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCQ2671901.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            22617..23942
                     /gene="rimO"
                     /locus_tag="OHO85_002542"
     CDS             22617..23942
                     /gene="rimO"
                     /locus_tag="OHO85_002542"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCQ2671902.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(23989..25317)
                     /locus_tag="OHO85_002543"
     CDS             complement(23989..25317)
                     /locus_tag="OHO85_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCQ2671903.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(25325..>27540)
                     /locus_tag="OHO85_002544"
     CDS             complement(25325..>27540)
                     /locus_tag="OHO85_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCQ2671904.1"
                     /translation="RFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKM
                     EMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKR
                     PWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNE
                     YLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVP
                     NNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVN
                     TLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGF
                     IKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDI
                     VTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYA
                     EKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDI
                     NTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQR
                     ASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFY
                     LNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIF
                     DLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         7040 a         7102 c         6578 g         6820 t
ORIGIN      
        1 tttaatcata tttattgcat ataaattcac ttgatggcat tgttatccca tgccgcagac
       61 acggccaaaa atcataagat tgacagacgg gagttccatc atgaaaaagt tagttcttgc
      121 cgctttactt gcttccttta ctttcggtgc ttctgccgca gagaaaatca attttggcgt
      181 ttcagccacc tatccaccct ttgaatctat aggtgctaat aatgagattg tcggctttga
      241 tatcgatctg gcaaaagcct tgtgcaaaca aatgcaggca gaatgtactt ttactaatca
      301 cgcgttcgac agcctgatcc cgtcactgaa attcagaaaa tatgacgccg taatctccgg
      361 tatggatatc acgcccgagc gtagcaagca ggtatcgttt accacgccct actatgaaaa
      421 ctcagccgtc gtgattgcca aaaaagatac ctacaaaacg tttgccgatc tgaaaggcaa
      481 acgtattggg atggaaaacg gcaccacgca ccagaaatat attcaggatc agcaccccga
      541 agtgaaaact gtctcctatg acagttatca gaatgccttt atcgatctga aaaatggtcg
      601 tattgatggg gtatttggtg acacagcggt ggtaaacgaa tggctgaaaa ccaatccgca
      661 actgggcgtt gctactgaaa aagtgaccga tccgcaatac ttcggcaccg gtctgggcat
      721 cgctgtacgt ccggataaca aagctctgct ggaaaaactg aataacgcgc tggcagcaat
      781 taaagctgac ggtacatatc agaaaatcaa tgaccagtgg tttccgcagt aattgagact
      841 gatgacaaaa gtaagattgc ctgatgcgct gcgcttatcc ggcccacatc aactctgcaa
      901 tgtattgatt tagcatgatt ttgtaggccg gataaggcgt acacgccgca tccggcatga
      961 acaaagcgca cctgatcagc aatctgaagc cgcacctgcg gcttttttat tgcttcacca
     1021 gcagcgtcag cacttcatag tgcgcggtgt gcgggaacat atcaaaaagc tgtacccgtt
     1081 cgatgcggta acctggcagt tcgcggatat ctttcgccat ggtttgggcg ttacagctgg
     1141 agtagataat aaaacgcggt gccattgttg agagataatc acatagcggt ttaccaatgc
     1201 cgcggcgcgg cggattaacc agcaccagct ccggcacctc cccctgagcg gtggcaaact
     1261 gagtggagtc cagcgcctga aattgcaaac gcgttaagcc cagttcagcg gctgactgct
     1321 ttgcacaggc aatggcctct gatgcaattt cgatcccggt taactgcatg tcaggcgtcg
     1381 cgcagtgtaa accaaagcca cccacaccgc agaacagatc ccacatatgt ttaactggca
     1441 gctgccgtac ccagtcgcgc gcggtagcgt acagctggct ggcgaccgcc ggattagtct
     1501 ggaagaaact ttgcggacgg atccacagcg gtacgtcatt aaaacgctct gccagcgcct
     1561 gttgttcagt aaggtagatc tccgtctccc cttccataat cgccatatgt accggctgaa
     1621 tattgacggt aataactttc agttgcggta gttgttcctg taaccacggc agcgccttac
     1681 gcagttgtgc cagtttggtt tcaaaacgca gtacaaagcg cagcatcatg ccgccatcgc
     1741 tctggctttc agtcagcaga atgtatttca gttcgccacg cttacgcgcc acgttgtagg
     1801 gtgtcagccc cgcacgggcg ataaacggtt ttagcgccgc aaaaacgggc gcaaatgagg
     1861 caggataaag cgggcagtca caaaggtctt ctggtgtgcc gtcgcgatgc agcataccga
     1921 gcagtggttt ttcaacgcta ccactcacca ccattttggc tttattacga aacccttgtt
     1981 ccgggcctga caccggcgcg caccattcct caaccggaaa atcggcgagc agatttttaa
     2041 gatcggcggt tttagcggag agttgctctg gaatcggctg cgttatccac tgacaggaac
     2101 gacagcgacc cgcgtcgtaa agtgcgcact gcatacattg accttcacat catcaggggc
     2161 gacgattata cacattattg caactggaag aaccgccgac tggtcgaggg aacgaacagc
     2221 agcatcagga tgagcatatc cggcagcttc tgcagcatca ggctatggaa gatttcacgt
     2281 tttgattcac cgggaatgct gaacagctcc ggataaccgt accccagcga agccgcccac
     2341 aagtaacttg cggcggtgat ttgtgtcagc agatatagcc agcgcgccca gcgacgccct
     2401 ttcaccagtg aaaacgcaca ccagatctca atgaaaacca gcaccagact gcttaaaaag
     2461 accagcgtta aactccaggt ttgtacgctg cgatgaatga actcgccaat accgcgcacg
     2521 cccagcgtat tgaaaatcat cagcacgtcg aggccacgga tcataataat ggcgagcgcc
     2581 gctacctgca ccagcgcagg cacattcagg cgagcatgag atgaagatgt tttcttaaaa
     2641 aatcccaacg tttcgtcttc catgaaaaca atgccgcggc atgcgcggca ttatgtagcc
     2701 aggttggcaa attttagtgt cttcagccat gtcttgcacg ctggatatcg cgtatccgct
     2761 gtttttctgc gcgagccatc agataccagg cgataaatcc gacaattccg accgcaccga
     2821 gaattaacgt tgcaagggcg ttgatttccg gattcacccc catccgcacg ctggaaaaga
     2881 ccagcatcgg taacgtggtg gctcccggcc cggaaacaaa gctggcgatc accagatcat
     2941 caagcgacaa agtaaaagcc agtaaccagc cagaaatgat cgcgggcatg atcatcggta
     3001 gcgtaatgac gaaaaacact ttcagcggcg tcgcaccgag atccatcgcg gcttcttcta
     3061 tcgagcgatc cagttcccgc aatcttgaag aaataacgac cgccacataa gccgtacaaa
     3121 aagtgacatg cgccagccag atggtgagca taccgcggtc cgcaggccag ccaatggcat
     3181 gagcaagcgc gacgaataat aacaacagcg acaagcccgt gatgacatct ggcattacca
     3241 gcggcgcggt gatcataaag gcaaagccat ttgatccgcg aaacctgcca aagcgcacca
     3301 gcaccaccgc cgcaatagtc ccgaggatcg ccgccgccgt tgccgcacag gcagcaattg
     3361 tcaggcttaa accaaccgca ctcatcatcg cgtcatcgcg caataactca ccataccagc
     3421 gcgttgacca gccggcccac accgtcacca gcttcgagct gttaaacgaa tagataacca
     3481 gcatcagcat tggcgcgtag agaaaggtaa agcccagcag caaaatcaca atccgccagg
     3541 gtgaacgaac taccggtaaa ttattcatcc gtgttctccc acgctttttt gctggtgttt
     3601 gtggaaccac attatcggca caattagcag cagcaacatg atgatcgcta ccgccgaggc
     3661 caccggccag tcgcggttat tgaaaaactc ttgccatagc acgcgcccga tcatgatgct
     3721 gtccgggcca ccgagcagtt ccgggatcac aaactcgccc acagccggga taaacaccag
     3781 catcgatccg gcaataatcc cacctttagt cagcggcacg atcacagtaa agaacgtttt
     3841 cagcggtcgt gcaccgagat ccagcgctgc ttccaccagc gaataatcaa tacgaatcaa
     3901 cgcggtataa atcggcagta ccataaacgg cacgtaggcg taaacaatgc caatataaac
     3961 tgccagattg gtatgaagaa tggtcagcgg ttgatcgata acccccagcc acagcagaaa
     4021 attattcagc acaccgttgt tttttaatat tcccatccag gcataaacgc ggatcagaaa
     4081 cgaggtccac gacggcagga tcaccagtag taataaaata ttacgggtcg aaggcttact
     4141 gtgcgccacc gcccacgcca gcggatagcc gatcagtaaa cagcaaaatg tcgaaatcgc
     4201 agccacctgt agcgactgga gataagcatc gaaatagagc ggatcgtcgg tcagttgcag
     4261 aaaattaccg agattaagag tgatggacag ttgcccgtca gcccactcca tcagttcggt
     4321 ataaggtgga atagcgcgcg ccatctccga caggcttatt ttaaagacaa tcagaaatgg
     4381 cagcagaaac agcaagatca accagatata tggcaacgca atgaccagtt tgcgcccatg
     4441 cttcatttgc agctgcgaca accacagctt aaatccgccc ggcggtttcg actgggcagc
     4501 aggttcaagt gtattcatct ttgctcctta aaccgtcagc accacacagc tgtccacttc
     4561 ccagcacaaa cgcacttcgt cgccccaggt cggtaaccct ttacgatgac gatgggcgtt
     4621 ttgtagctgg gcgctaatca tctgcccact tttcagacga acgtgataca ccgaaagatc
     4681 gccgagatag gcaatgtgta tcacctcccc caccgcgaag ttacaaccat tggcgggcgg
     4741 ctcttcgcaa agcatgattt tttccgggcg cagcgctacg tgtaccggca cgttatcgac
     4801 cactgaagca tccgcatcga ctttcagtgg atgcaccagc cccggcgaat caagcaccag
     4861 gccatcttcc tgacgctctt tgagtacacc ttcaaagaca ttaaccgaac cgataaattc
     4921 agcgctgtag cgggtagttg gatgctcgta gatctcttcc ggctcgccaa tctggacaaa
     4981 tttcccgcga ttcataatgg cgatacgccc cgccatggtc atcgcctctt cctgatcgtg
     5041 ggtgaccatc acacaagtca caccgacgcg ctccagaata tccaccactt caagctgcat
     5101 ccggtcacgc agctttttat ccagcgcgcc catcggctca tcgagcagta atagtttcgg
     5161 gcgtttggca aggcttcggg ccagggccac acgctgtcgc tgaccaccgg aaagctgatg
     5221 cggtttgcgt ttggcgaact cctgcatgtg caccaggccg agcatctcat ggacccgact
     5281 ggcaatttcc gctttcggta gtttgtcctg tttcaggcca aaagcgatgt tctgttccac
     5341 ggtcatatgc ggaaacagcg cgtaagactg aaacatcata ttgatggggc gcaggtaagg
     5401 cggcacctgt gacaaatcga cgccatcaag cattatctgt ccggcagaag gttgttcgaa
     5461 acctgccagc atacgcagca gcgtggactt gccacagccg gatgcgccca gcagcgcgaa
     5521 gatttcacct ttgtagatgg tcaggctgac atcatccacc gcatgttgac catcgtagga
     5581 tttggtgagg ttgcggattt ctaatagcgg cgtcagcgcc ttacgggttt tcgcctgcgg
     5641 gcgagggata gcgtcattca cggctgctct ccggcattga atgtgtgcaa gcgcaccatg
     5701 atggtgcgaa ttgccggcgg gtgtggtgcg cccgcccggc atctaaggct gcggattatt
     5761 ttccgctctt cactttggtc cacgcgcggg tgcgcacacg gtcgattttc gggtcctgca
     5821 ctttcagagt gaacagcttc gcacgaacat ccgcaggcgg ataaataccc gggttctcac
     5881 ggacttccgc actcaccagc ggcgtggctg ctttattggc gttggcatag aacacatggt
     5941 cggaaatatg cgccaccaca tccgggcgca gcaggtaatt caggaactga taggcttcgt
     6001 ctttattttt ggcatcggca ggcatggcga atacatcaaa gaatgccatc gccccctctt
     6061 tcggaatcga gaacgagaca ttcacgccat tcttcgcttc cttcgcgcgg tttgacgcct
     6121 gccagacatc gcctgcccaa ccaatcgcta cgcagatgtc accattcgcc aggtcattaa
     6181 tatattgcga tgaatggaaa taacgaatgt tcgggcgcag ctttaacagc agatcggtag
     6241 ccggtccggt gtaatcatct gctttagtgc tgttgggatc tttgccgaga taattcaaca
     6301 cggtagcaaa aacttcttct ggcgcatcca ggaaagagac accgcagctt ttcagttttt
     6361 ccagattttc aggtttgagg atcaagtccc agctatcgac gggcgcgttt tcgcccagca
     6421 cagctttaac tttatcaacg ttatagccaa tcccggtcgt cgcccacata tagggcatag
     6481 caaatttatt gtcgggatcg tgtttggcga ccagcttcag cagctccggg tcgagattct
     6541 tccactccgg caatttgctt ttgtccagcg gctggaaaac tcccgcagtc aactggcgtt
     6601 caagaaagct ggcagatgga accaccaagt caaagccggt gctacccgcc attaatttgc
     6661 cttccagtac ttcgttagag tcgaaaacat cgtagacgac tttaataccg gtttcttttt
     6721 caaaattggc caccgtgtcc ggggcgatat aatcagacca gttataaacg tggagtgttt
     6781 tttgttcagc cgcgagcgtg ccgacagaga cggccatcag agcacccgca gccagacccg
     6841 aaagccattt tttatttaag gcggtcatat ctctttcctt ctgaaagttc gttaacaaac
     6901 gaacaaaagt tgtcacacta aaagatatgc aataaacgtg catatttttt atcggcgggg
     6961 tgtaagaaaa gagaactctc tcccggccag cactgctcgc tatataaaag catcgcaaga
     7021 ataactttag ccagggtttg agactatagc tcggattaaa aaacggtatt taacaaattt
     7081 ttatgcgtat ttgctctatg tgataaagcc aaatcgacac aagagaagtg aaataatctt
     7141 taagaaaagg ttaaaaacag cagataattt tgcagcgcgc agccactgca ccagcgactg
     7201 cgtgctcgaa aggagttatc agtggaggaa atagttttcg gtaacgttgt tttgcccttc
     7261 atcgtcagtc ggtaacagca gttgatgggc attagcttca agaatgacca tcgaaatctg
     7321 ctcttcgctc tgacgcacga accatgcaaa ctgctcatag gttacgccct gcataacgga
     7381 taaagactgg caaaccacca gctttggcag attatcatcc tgcatatcaa gaaatgcttt
     7441 cacggtcagc gaactggcat tgatggcaga taaatccgcc gccagcggca atacggctga
     7501 aggtcggact tccgccatgg cagaaaacag gatcgtgtta tcgatcagat cgattttggc
     7561 atcaaagaca ccgtcgaaat tctgcatatg gggcagatgc agagcctgac agttatcaca
     7621 ttcaaaaaaa ctcatcccca ggtcatcgag ccattgacgc agcgtatcca gaccaggaac
     7681 gaccagcgat gtcataattt tgtacaacct ctttcgataa aaagaccggc acagcttacg
     7741 caaaaagcgc aggcaaaacc atgatcagta atgtgattgc gactaaccac ccgttttcag
     7801 gcaatattcg gtcgtagcgt ggcgttcgat ccagcggatc attttacccg cgatgtcgat
     7861 accggtggtt ttttctattc cttccagccc cggcgacgca ttcacctcca tcaccaacgg
     7921 cccgcgatta gcacgcagaa tatccacacc agcaacgtcc agcgccatcg ttcgcgcggc
     7981 ttttatcgcg atttcacgct cctgtggtgt gatactggcg acgcttgccg cgccgccacg
     8041 atgcaaattg gaacgaaaat cgccctcttt cgcccgccgt tcaatcgcag cgaccacttc
     8101 atcgccaaca accagacagc ggatatcgca cccttgcgcc tctttgatat attcctgcac
     8161 cagaatatgc gcgttcagac cgcggaaagc gtctatcacg ctttccgccg cctgacgcgt
     8221 ctccgccagc acgacgccaa ttccctgcgt gccttcaacc aacttgacca ccagcggcgc
     8281 accaccgacc atgtcgatta aatcgctggt atcatccggc gaatgcgcaa tgcccgtgac
     8341 aggcaggtcg atgccctgac gcgccagcag ttgcatggaa cgcaatttgt cacgcgcccg
     8401 ggcaatggcg accgactcat tgagcggata gctccccagc atctcgaact ggcgcagtgc
     8461 cgccgtccca taaaaggtaa tggcggtgcc aatacgcggg atcactgcgt caaaatgggg
     8521 taacttgcgg cctttgtagt gaatagaaga cgccgcagga tttatgttca tgtagcaaga
     8581 aagcggatca agaatttcaa ccaggtgacc gcgctgtata gcggcttcac gcagccgctt
     8641 acacgaatag agcgttccat cccgggacaa tatggcaatt ttcaccctgc acctctctgt
     8701 cagacctggt caaaagcccg cgtatcatac atcgacgtgg cggttttagc gcgtcgccca
     8761 accctgtttg tgcaaataat ccagaataaa tgggcggctt tctttaatga ttgttcggcg
     8821 gatatgatcg ctccaggtat cccggcgatt attgctgcca cgggtgaggt aatattccgc
     8881 cagttgctcg tcatactgcg ccagtgcgcc tttatccagc ggttgatagc tgttttcatg
     8941 caccaaaatg gaggccggta aacgcggctt aagatccgga ttatccgcag gccagccaag
     9001 gcacagccca aacagcggca gaacatgctg cggtaattta agcagtttcg tcaccgcttc
     9061 aatattattg cgcaggccgc cgatatatac cccgcccaat cccagcgatt ccgctgcgat
     9121 taatgcattc tgcgccatca ttgccgtatc aacgacaccg agcaacagtt gttccgccaa
     9181 gccgagctga gcatccggac agatctgtaa atggcggtta aagtcggcac agaacaccca
     9241 gaactccgcc gcttgcgcta cgtgtttttg cccgccggtc agcgtcacca gttcttcacg
     9301 taacgctttg tcggtaatgc gaataatgct actgcactgc aaaaaactgg aactggacgt
     9361 cgcacgggcg ctgttaataa tcgcctcacg ctgcgcttcg gaaatgggtt catcagtgaa
     9421 atggcgaatg gagcgatggc cacaaataag ttcaatggtt ggcgtcatta tctttttctc
     9481 tttctgaacg tgaatattgc ggtggacggt tcatcagctg tggggcaaga cgttttgcca
     9541 cctgaagaat aaccaccacc gcagcgggaa gcatgagcaa aacaccgaga aaaatcatca
     9601 gaatctgcac ttctggccga gaaaatggct caggcagcga cagggagtcg cttaccgaca
     9661 gcagcgccac cgccagtagc atcattccga taaattccag tatcaacacg cctttaggca
     9721 atttaccgat cgcgcgcata cgcttccctc tgcaaagtga gccttcagtc taaaactttt
     9781 cactgtattg tgtttaacag ttatagcttt tagcaattaa tgcaacaggt taaacctact
     9841 ttcagcgaat acattttagc gtgatcatta caggcataaa tctatgagga gagaaataat
     9901 gcaaaccgtt atttttggtc gttcgggttg cccttactgt gtgcgtgcaa aagatctggc
     9961 tgagaaattg agcaatgaac gcgatgattt tcagtatcag tatgtagata ttcgtgcgga
    10021 agggatcact aaagaagatc tacaacaaaa ggcaggtaaa cccgtagaaa ccgtgccgca
    10081 gatttttgtc gatcagcaac atatcggcgg ctataccgat tttgctgcat gggtgaaaga
    10141 aaatctggac gcctgatcgt ctgacaagcc ctcgcgttga gggctttact gattttttct
    10201 gtgctgtggt ttaaacaaac tactgataaa taagaaacac agtgccccca gcgcacacca
    10261 gaacaccgcg cttagtaacc atgccagctc ttgccagaat gagcgcgtcg gtgaaaaaaa
    10321 cagccgcata atgagcatcg aacagggtgc cgccagcatt gcgccaaaca gaggtttcag
    10381 gacttctcta cgctgtgaaa agaagctggc gactgctcca ggaagaatga aaaatagcaa
    10441 gccgatttca ggatgcccgg cagcccgaaa agcgcctttc atgtgcgtcg ccagaaaaag
    10501 gcacaccaca atgaagagga caaaacagca gattgccccc gcccaacgtt gtttatgttt
    10561 cactcgttcc tcctgacact gcgtctatcg aacacatttt tcgccagtgt ggcgttcagt
    10621 aagataaagc cgcttcgcat tccatgctaa tataggccaa cgcaattcat atagccgttg
    10681 atacctaatg tgattacact agtaaaatat attgttactt tactatcgtt taggtgcgct
    10741 gaatgaatct gcgccctgaa ttctggtaaa aaacattatc gtaaattacc atttctttca
    10801 acagcttact agtaaacaag aagttagcct ccgtgaatat aaacgtcgcc gaattgttaa
    10861 atgggaatta cattctgtta ttatttgtgg tcctcgcgct tgggctatgt ctcggaaagt
    10921 tacgacttgg ttcgatccaa ctgggtaatt ccattggcgt tttagtcgta tcgctgttat
    10981 taggccaaca acatttcagc attaacaccg atgcgcttaa tcttggcttt atgctgttta
    11041 ttttctgcgt cggggtcgaa gccggaccga actttttttc catttttttt cgcgatggga
    11101 aaaattacct aatgttagca ctggtgatgg ttggcagtgc gctggtgatc gccttagggt
    11161 taggtaagct gtttggctgg gatattggcc tgacggccgg tatgttagca ggctctatga
    11221 cgtcgacacc ggttctggtc ggtgctggcg atacactgcg tcattccggc atggaaagca
    11281 ggcagctctc actggcactg gataatctga gcctcgggta tgccttaacc tatttaatcg
    11341 gtctggtgag tttgattgtt ggtgcgcgtt acttgccgaa attgcagcat caggacttac
    11401 agaccagcgc ccagcaaatc gcccgcgaac gtggcctgga cactgatgcc aaccgtaagg
    11461 tttatttacc ggtgatccgc gcctatcgcg tcggcccgga actggtggcc tggaccgacg
    11521 gcaaaaatct gcgtgaactg ggtatttatc gacaaaccgg ctgctacatt gaacgtattc
    11581 gacgtaacgg gattctggca aatccagacg gtgatgccgt gctacaaatg ggcgatgaaa
    11641 tagcgttggt aggctatccc gacgcccatg cccgactcga tcccagcttc cgtaacggta
    11701 aagaagtttt cgatcgtgac cttctcgaca tgcgtatcgt cactgaagaa gtggtcgtta
    11761 aaaaccataa cgctgtaggt aaacgtctcg cacaactgaa gttgaccgat cacggttgct
    11821 tccttaaccg cgtcattcgt agccagattg agatgccgat agatgacaac gtcgtgctta
    11881 acaaaggtga cgttttacaa gtcagcggcg atgcccgccg cgtaaaaacc atcgccgatc
    11941 gcatcggctt tatctcgatt cacagccagg tcactgacct gctggcattc tgcgccttct
    12001 ttgttattgg gctgatgatc gggatgatca ccttccagtt cagcacattc agtttcggca
    12061 tggggaacgc tgccgggttg ttattcgccg gaattatgct gggctttatg cgtgctaacc
    12121 acccgacctt cggttacatt ccgcaaggtg cattaagcat ggtgaaagag ttcggcttga
    12181 tggtgtttat ggcaggcgtt ggtctgagcg ccggtagcgg tattaataac ggcctgggcg
    12241 cgattggcgg tcagatgttg attgccggat taattgtcag tcttgtgccc gtggttatct
    12301 gtttcttgtt cggtgcttat gtattgcgaa tgaaccgcgc actgttgttc ggcgcaatga
    12361 tgggcgcacg cacctgcgcg ccggcaatgg agatcatcag tgatacagct cgcagtaaca
    12421 tccctgcgct gggctatgcg ggcacctacg caatcgccaa cgtcctgctg acgctggcag
    12481 ggacaatcat cgtcatggta tggccaggat taggataaaa ctgaagttgc cctgaaaatg
    12541 aaattttttt gcacaaccgc agaacttttc cgcagggcat cagtcttaat tagtgccact
    12601 gcttttcttt gatgtcccca ttttgtggag cccatcaacc ccgccatttc ggttcaaggt
    12661 tgatgggttt tttgttatct aaaatttatc tactaccctg caaccctctc aaccatcctc
    12721 aaaatctcct cgcgcgatag cggcttacgg tcggtgacaa aatgcagcgt catcccctca
    12781 ataaacgcat caagcgcgcg ggcggttccg ggttcaaacc attgttcgag cgtttgctga
    12841 ctgcgctgca tccagttttg cattaccgtt tttaatagcg gttttcggct agccagcgcg
    12901 tagagctggt acatcagctc catgttatcc ggcgttgcaa cctgtgagct gtagatcata
    12961 tcggtgatag cctggcatgc gccttgagca tcactaacat cgctaaaaaa tgcctgatat
    13021 tgccgggaca taatctcagt aaaactgctg aacgcctcca gcaacaactc atcaattcct
    13081 gaaaaatagt aggtcatcga tcccaacggt accccggcaa gggtagcaat tttgcggtgc
    13141 gtaacagcat gtattccgta aagtttcacc gcctccagcg tggcctggat aattttttct
    13201 cgccgttgcg gatcgttagc gcgacgcatg tcatttcctc tgtctgcaat tgtgtacaaa
    13261 tgtacacaac cttgctaatg ttgtcttccc tcttcttatt ttgacgtgat ctatgaccgt
    13321 aaattcttca cgtaatgcat tgaaacgccg aacttgggcg ctgtttatgt tcttcttttt
    13381 gccaggcctg ttaatggcgt cctgggcaac ccgtacgcct gctatccgcg atattctctc
    13441 tgtctcgatc gctgaaatgg gcggtgttct ctttggtctg tcgatcggtt cgatgagcgg
    13501 tattctctgc tcggcgtggt tagtgaaacg ctttgggaca cgtaatgtca tcctggtcac
    13561 gatgtcctgc gcattgatcg ggatgatgat attaagtctg gcactctggc tgacatcgcc
    13621 cctgctcttt gccgttggtc tcggcgtctt tggggcaagt tttggttctg cggaagtggc
    13681 gataaacgtt gaaggtgccg ccgttgagcg agaaatgaat aaaacggttt tgccgatgat
    13741 gcacggtttt tatagcctgg gcacgctggc aggcgctggt gtcgggatgg cactgacggc
    13801 ctttggcgtt ccggcaacgg tgcacatttc attggcggcg ctggtaggta tcgcgcctat
    13861 ttatatcgcc attcaggcaa tccctgacgg tacgggcaaa aatgctgccg atggcaccca
    13921 gcatggcgaa aaaggcattc ctttttatcg cgatatccag ttgctgttga ttggtgttgt
    13981 agtgctggcg atggcctttg ccgaaggttc tgccaacgac tggttaccct tattaatggt
    14041 tgacggtcac ggttttagcc ctacttccgg ctcgctgatt tatgccggtt ttaccctggg
    14101 gatgaccgtt ggacgcttca ccggtggttg gttcatcgac cgttacagtc gcgttgccgt
    14161 ggttcgggcc agtgcactaa tgggggcgtt gggtattggg ctgattattt ttgtcgatag
    14221 cgcctgggtc gcaggagtgt ctgttgtact ttggggactg ggtgcctcgc tgggcttccc
    14281 gctgaccatt tctgccgcca gcgataccgg ccccgatgca ccaacccgcg tcagtgtggt
    14341 agcaacgacc ggttatctgg ctttcctcgt cgggccgccg ctgctgggct atctcggcga
    14401 acattatgga ttacgtagtg caatgctggt tgtactggcg ctggttattc tcgcggctat
    14461 tgtcgcgaaa gccgtcgcca aacccgatac caaaacgcag acggcgatgg agaatagttg
    14521 atgagcatta aattaattgc ggtagacatg gatggtactt tcttaagcga tcaaaaaacc
    14581 tataaccgtg agcggtttat ggctcagtat cagcaaatga aagcacaagg cattcgcttt
    14641 gtggtcgcca gcgggaatca atattatcag ttgatctctt tcttccctga aattgcgaat
    14701 gaaattgcct ttgtggctga aaacggcggc tgggtagtga gtgaaggcaa agatgttttt
    14761 aatggcgagc tgtcgaagga tgcctttact actgtcgtgg aacatttgct gacgcgcccg
    14821 gaagtggaaa ttattgcctg cggaaaaaat agcgcctata cgctcaaaaa atatgacgat
    14881 gccatgaaaa cggtggcgga aatgtattat caccgtctgg aatacgtcga taactttgac
    14941 aacttagaag atattttctt taagtttggc ctgaatcttt ccgatgaact gattccacaa
    15001 gtacaaaaag cattacatga ggccatcggc gatattatgg tgccagtcca caccggcaac
    15061 ggcagcatcg atctgattat ccccggcgta cataaagcca atggccttcg ccaactgcag
    15121 aaattatggg gaatagacga cagcgaagtg gtggtctttg gcgatggcgg taacgatatt
    15181 gagatgctgc gtcaggcagg ctttagtttt gcaatggaaa atgccggcag cgcggtcgtc
    15241 gcagcggcaa aataccgggc aggctccaat aaccgtgaag gcgtactgga tgtgatcgat
    15301 aaagtgctta aacacgaagc gccatttgac caataaaaac ggcagcacgc ccggcaacac
    15361 aatacctgaa tgaaggctgc ataattcgcg gaaattaacc cgctgcatta tttcaggcga
    15421 aatgattatg aaaaattgtc tactgttggg cgcgctttta atgggcttta ctggtgtcgc
    15481 gatggcgcaa agtgtcaccg tcgatgtgcc gagtggatac aaagtggttg tagttcctga
    15541 ttcagtcagc gttccacaag cggtcagcgt cgcgactgtg ccgcaaaccg tctatgtggc
    15601 tcctgcgcca gctccggctt accgcccgca tccttatgct cgccatcttg ccagcgtagg
    15661 tgaagggatg gttatcgaac accagattga cgaccatcac cattaattcg agaatgcctg
    15721 atcgcacaaa gcagtcaggc atttttttac ccttcgtgag aatttcccat ctgtttatct
    15781 tttaaaaaga taaccatcag cgacagccac aaaattccgt tgacaaggtt gaagagatta
    15841 aactgtccgt tgcccccgtt cagccaggca tgtttgctga tttcaatacc aacggtaaag
    15901 atcagcattt gcagcattcc catcgccgca gaaaccgtac ctttactcat atcgctggca
    15961 aacagggtta atcgcaccag tcccgcattc gccagaccaa taccgaaagc ataaatactt
    16021 aacccggcag tcatccataa atacgcgtgc gatgagataa ccgttgccgc agcagcgacc
    16081 aacagaccaa tcataatcgg ccagccgccc ataattatca gcgaacgtac ggtgcggcgc
    16141 gaggtcaaac gcgctaacaa caagttaccc gcaattaacg ccccgaaaat aggcacttgc
    16201 agcaagccat attcatagct gctcaactgc tcgccggtaa tgatgataat cggcgactgg
    16261 gcgatccacg ccagcaatgg caggctaacg aatcccagcg ccagcgcccc cgccacaaag
    16321 cggccgttct ttagcaccag cttatagtca cgaccgagtt ctttcagtga cagtttctcg
    16381 cctatacgcg tggcggtttc aggcatagct cgttgcagac cgaaaaagga gatcgctgcc
    16441 aatgcggcaa acaaaacaaa catcccctcc cagggcagca catggatcca cgccgcgccc
    16501 accagcggac caagtagcgg agcaatcagc gccacgttcg ccatcagcgc ggtgatcttg
    16561 atacaaaccg cctcttcgaa ggattcctga attgcggcgt atcccacagc gccaatgaaa
    16621 cagaggctta tgccctgcaa gaagcgcaac agggtgaatt gttcaatgtt ttgcgccagc
    16681 aatattgcca gacaggtgat gataaaccac accacccccg ccagcatcac cggacggcga
    16741 ccaatacgat ccgacagcgg ccccaggagc cattgtaaaa acatcccgcc cgccagatat
    16801 gcggtcattg aagtaggaac ccaatcaatg cccgcctgat attgttccac cacggccaac
    16861 ataccgggtt gaatcatatc gttgccgata taggttgaaa attcgtaaag caccagacag
    16921 agagggaaaa gtaacgcctg acgtccaagc ctggcaccgg aagctaattt attttgcatg
    16981 caatttcttc gccaataata atcgcgcaga gtttaataaa agcacagcta acgagaaagc
    17041 gaattttgta gctgaaacca cggttaagca cattcttaca ttattgcgag tatagctacg
    17101 ctttctttaa gttttattta acctatgccc gttacaatta cccaccgtaa acaggccgct
    17161 tgagggaaat aagacgatgc cgctttaccc agtttaacct gcactttatt ctcaacgact
    17221 tgcctgtatt ggctcccttt taatcacttt gcgtcgggaa gttatgctgg aaaatttgaa
    17281 tctctctcta ttctctctta ttaacgcgac gccagactcg gctccgtgga tgatctcgtt
    17341 ggcgattttt attgctaaag atttgattac cgtggtaccg ctgctggccg cggtgctttg
    17401 gttgtggggg tttacagcac aacggcaact ggtgataaaa atcgctatcg cgctggcggt
    17461 cagcctgttt gtgtcctgga cgatgggaca tctttttccg cacgaccgac cctttgtcga
    17521 aaatatcggc tataacttcc tgcatcatgc ggcggatgac tcattcccaa gcgatcacgg
    17581 tacggtgatt ttcacctttg cactggcatt tttatgctgg catcgcctgt ggtccggctc
    17641 acttttaatg gtgctggccg tcgtcattgc ctggtcgcgc gtttatcttg gcgtccactg
    17701 gccgctggat atgctcggtg gattgctggc aggtatgatt ggctgcctta gtgcccagat
    17761 tatctggcaa gcgatggggc ataaactcta tcaacgtctg caatcgtggt atcgcttctg
    17821 ttttgcatta ccgatccgca aaggctgggt gcgtgactga tctctgctaa aaagtgtagt
    17881 attgagcggc tcgcttcaat aactattcag agggattatg gaaacacgtc gcgaagagcg
    17941 tatcgggcag ctgctgcaag aattaaaacg cagcgataag ttacatctta aagacgccgc
    18001 cgccctgctt ggggtttcgg agatgacgat tcgtcgcgat ctgaacaacc acagtgcgcc
    18061 cgtcgttttg ctcggcggct atattgttct ggaaccgcgc agtgccagcc attacctgtt
    18121 aagcgatcaa aaatcccgcc tggtggaaga aaaacgccgg gcggcaaaac tggctgcgac
    18181 actggtagaa cccgatcaga ccctcttttt tgactgtggc accaccacgc cgtggattat
    18241 tgaagcgatt gataatgaaa tcccttttac cgccgtttgt tattcgctaa atacctttct
    18301 ggcgctgaaa gagaaacccc attgccgcgc atttctgtgc ggtggtgaat ttcacgccag
    18361 caacgccatt ttcaaaccca tcgattttca gcaaacgctg aataattttt gcccggatat
    18421 cgctttttat tctgcggcgg gcgtgcatgt cagtaaaggc gctacctgtt ttaatcttga
    18481 agagttgccg gtaaaacact gggccatgtc gatggcgcaa aagcatgtgc tggttgtcga
    18541 ccacagtaaa tttggcaagg tgcgtccggc gcgcatgggt gacctgaaac gctttgatat
    18601 tgtggtgagt gattgttgcc cggaagatga gtatgtgaag tacgcgcaga cgcagcgcat
    18661 taagttgatg tattaatgac gtataaccgg atgacgtttc gcgccatccg gttatcagaa
    18721 gattaagaga accagctgcc gaaccactga tggaatttca tcatcacgaa atcccacatc
    18781 cgaccaaaga atccgccctc ttccacattt tccatcacga tcagcggacg ctgctcaatg
    18841 gatttaccgt taagctggaa atcaatggtc ccgacaacct gacctttttt cagcggtgcg
    18901 gtaagctgcg gttccgttaa cgtgtagctc gccttcaggt ttttcagctg cccacgcggt
    18961 atggtcactg agcccgcttc gcctgcccca agattcactt cgctcttatc accaaaccag
    19021 acgcgctgag tcacaaaggt ggcatcaggt ttaattggcg tcacggtttc aaagaagcgg
    19081 aaaccccagg tcaataattt ctcagactca ttaaaacgga tacggtcagt tttcgccccc
    19141 agcactacgg agattaaacg catatcgccc tgggtagccg aagcaaccag attatatccc
    19201 gcgcctgccg tggttcctgt cttcatgcca tcaacattca gattgctgct ccacagcaga
    19261 cggttacggt taggctgacg aattttgttg aaggtgaact ctttctcttt atgaatggcg
    19321 tactcttccg gcacatcgtg gatcaatgct ttacccagca atgccatatc gcgcgcggta
    19381 ctgaactgcc ccggcgcatc cagaccgtgc accgtctgga aggtagtgtt ggtcagaccc
    19441 agttttttgg cataaccatt catcaaacca atgaatgact cctggctccc ggcaacgtaa
    19501 tcggccagcg caatacaggc gtcattaccg gactggataa tcacaccttt gttcaagtct
    19561 gccaccgaaa cctgatcgcc cggtttgagg aacattaccg atgaaccacg cagtgccgga
    19621 tttcccgtcg cccaggcatc tttaccgacc gtcaccatat cggtgagttt aatcttatcg
    19681 gccttaagcg cctgcccaac cacatagctg gtcatgattt tagtcaggct cgcgggatcc
    19741 agtttctcat ccgcgttgcc ttctgccagc actttaccgc tggcgtaatc cattaaaatc
    19801 catgcacgcg catccacgct cggcgcttca acggtttgtt ccgccgcgaa tgccgttggg
    19861 gcaaaaagga ataaaaatgc agaacccgct gcaagaccac gaaggagaga ggagtattgc
    19921 gtcataaatg ccacccgagt atccattcaa aaaaaatacg ttatatcgcc gtatgataag
    19981 ttcggtgatt aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa
    20041 agtttatgca atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc
    20101 ttaaaagtta actttacttc accatcggag tcctgttcag cctgtggctg cctgtcggaa
    20161 aaggggtgag tatgattacg ctgtggggtc ggaataattc aaccaacgta aaaaaagtat
    20221 tgctgacgct cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg
    20281 ggataaatca cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc
    20341 gtgacgacga aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg
    20401 cacagtacgg gcaaaaacgt ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa
    20461 aatggatgga ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat
    20521 tagtcagaac accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt
    20581 gcgacgccct gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg
    20641 acgagtttgg tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg
    20701 gcctgacctg gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc
    20761 ccgcagtgcg caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta
    20821 attccccact ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat
    20881 cacgaattcg ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt
    20941 tgacgctcat cacaatgaca tctttatctt tcagcgcgcc aataaataat ttttgctgcc
    21001 actgggggaa tttatcgctg ttatagaagg ccatgccgct cacagcgggc gaatctttcc
    21061 agtaaaaaac aggttgctcg gtcccggcga cgatctcccc tttcgcttcc ggtatcttaa
    21121 agcctgaata gttgattccc caggttgcca gcggccagcc gtagtttttg cctttttgcg
    21181 ggatattaat ttcatcacca ccgcgcgggc catgttcatt cagccacagt gcattactcc
    21241 acggattcat cgccattcct tgcggattac gaatgccata agaccagatc tcggcgcgca
    21301 caccggattc ctttataaaa ggattatcat ccgggatttc gccctggtcg gtcagacgca
    21361 ccagtttgcc ctgtaattta tccagatcct gcgccgtcgg gcgctgattg ttttcgccca
    21421 gagcaataaa aagataacct ttaccgtcga ataccagccg cccgccaaaa tggttgccgg
    21481 tagacagttt tggcatctgg cggaagacgg tgcggaagtc ggtcactttt gagagatcat
    21541 cacttaagcg gccataaccc acagcagttc cggctttgcc atcatcgcca acttcggaat
    21601 aacttaacca gatgcggcga gactgagcaa aatcaggcgc taaaaccacg tccagcaggc
    21661 cgccctgccc gtgcgcccaa acgtccggaa ctccggaaag cggcgcagat aatccttttc
    21721 ctgcttgcca gtgacgcaac tcgccgccgc gcagagtgat taacataccg tgattatcgg
    21781 gtaaaaaggc cagtgcccag ggatggtcga gtttgtcttg cagtacttcg acatttaccg
    21841 ttgcaggagc cgcccagaga gcggaagaaa gacaaataag gggcacaagg aaaaaggatt
    21901 gtcgatgcat aacgcctccg gattgactct ggcttaaagc gtagtcagtg gaggagataa
    21961 caaattcatt tttacaaaaa cttaaacatg aagggggaga cgctttctcc cccttagttt
    22021 tcaggccttc tcaagcatgg cgtgcttctg caggctctgg atactcagcg ttaagctcat
    22081 cagacaattt tcaagcttat cggcgttgac ggtaataaca gtcgggcaat catggtgccc
    22141 actcatcaaa catactgcgg ctgtcgctaa tgcttcttca gcatgatgaa gagcactcca
    22201 ctcttcctga tccagatgaa gattcaaccg cagcgattta tcgtgcagtt cgcgattcag
    22261 tttaaaaaag ttatctcgta gatgattgct ttcgctgacg gacatgtatc cttttgcctt
    22321 cctcagttgc acgtatgcag cgaggtcaac gcgtgcgtcg atcaaccggt tcagcagatc
    22381 gattcgctgt ctgtcaacga acatacttcg atcctcctct ttccagcctt gttgcaatta
    22441 aatagtatgg ctattttttg agcaaaatgc tgtccaggat caattcttca cgccgaatta
    22501 acaaaattta catttccttc gcctccgcca gcgaatgaga ttgcgataca aattttatcc
    22561 agaactctgt agaatccctg ccctgacctt tcctgtaact gaatattttt taagctatga
    22621 gcaaagtaac tccccagccg aaaatcggct ttgtttccct tggctgtccg aaaaaccttg
    22681 tcgattcaga gcgtattctc accgaactcc gcactgaagg ttatgacgtg gtaccgagct
    22741 atgacgatgc ggacatggtg atcgtcaaca cctgcggctt tattgacagc gcggtacaag
    22801 aatcactgga agccattggt gaagcgttga atgaaaacgg caaggtaatt gtgaccggtt
    22861 gtctgggggc aaaagaagat cagatccgcg aagtccaccc gaaagtgctg gaaattaccg
    22921 ggcctcatag ctatgagcag gttctggagc acgttcatca ctacgtgcca aaaccgaaac
    22981 acaacccatt cctgagcctg gtgccagaac aaggtgtgaa actgacgccg cgtcattatg
    23041 cctatctgaa aatttcggaa ggctgtaatc accgctgcac cttctgcatt attccgtcta
    23101 tgcgcggcga cctggtgagc cgtccgattg gcgaagtgtt aagtgaagcg aaacgtctgg
    23161 tagatgcggg cgttaaagag attctggtga tctcgcagga tacttccgcc tatggcgttg
    23221 atgttaaaca tcgtactggc ttccacaacg gcgagccggt aaaaaccagc atggtcagcc
    23281 tgtgcgaaca gttatcgaaa ctggggatct ggacacgtct gcactacgtt tacccttatc
    23341 cgcatgtgga cgacgtcatc ccactgatgg cagaaggcaa aatcctgccg tatctggaca
    23401 ttccgttgca gcacgccagc ccgcgcattc tcaaactgat gaagcgtccg ggttctgtag
    23461 atcgccaact ggcgcgcatc aaacagtggc gcgaaatctg cccggaactg accctacgct
    23521 caacctttat tgtcggcttc cctggcgaga cagaagaaga tttccagatg ctactcgact
    23581 tcctgaaaga agcgcgtctg gatcgcgttg gctgctttaa atacagcccg gttgaaggtg
    23641 cagacgccaa tgccctgcct gaccaggttc cggaagaagt taaagaagaa cgctggaacc
    23701 gtttcatgca gctgcagcag cagatttccg ccgagcgcct gcaagagaaa gtgggccgtg
    23761 aaattctggt gattatcgac gaagtggacg aagaaggcgc gattggtcgc agcatggcag
    23821 atgcaccgga aatcgacggc gcggtttatc tcaatggtga aaccaacgtt aagccgggtg
    23881 atatcctgcg tgtgaaagtc gagcacgccg atgagtacga tttgtggggt agccgggttt
    23941 aattaattca ggccgggcaa aatcatttgt ccggcctgcg aataatgatt actgccagct
    24001 aatctgagcc ctgccgtttt tcttactctt atacagccgc tcatccgcca gcattttcaa
    24061 gcgttcgacg gtggtacatt catttgaatg cgctacaccg atactggtac ttaaattaat
    24121 gcttcccacc tggaaatgaa cgatttcttt tagaatcgat agcaactctt ccagcgttcc
    24181 acccgtataa atcaccgcaa attcgtcacc gccgaagcgg tagattttac ctttatctac
    24241 cgggatctgt gatttaagga ttttcaccac tttcatcaac acttcatcgc ccagcaaatg
    24301 accgaaggtg tcgttaatgt ttttgaattt atcgacgtca aacaggcaca aataaccgcc
    24361 tttttccggc agcgctttta aatcttcgtc aaaagatttt ctgcccattg ctttggtcag
    24421 gtcgtccagg aacgccgagt tatacatact gtagagtttt ttgacatcgg cagccgaaaa
    24481 gaaaagcccg ccatccagta cgcgttcaaa atggttatta atgatggctt ctttctccac
    24541 aataccacga ttaatcactc tgcgtaccag atagaggcaa ctggtacccg atatcactgc
    24601 caccaatgcg gcaaagaaaa taatcaatgt acagacccgc aaataaacat cttttttata
    24661 cgcaaatacg tacaatccgg tcggcaaata ggcgaagtgc tccatcgctt ccgaaacggt
    24721 gaagatggcg tttttagcat tatattcatc accggtcacc ggtttaccgt tcttaaatag
    24781 agagaacatc aggttctcgc tgtccatcgc cagcgatttt gacagtaaag aaaaacgctc
    24841 cagaccaagc aaaaatatca gactacgatt gtgagttacc ggatagaggt aatagacgaa
    24901 ggttttaccg tcaaaagaga taaaccctgt atttttgaca tgtcgatact tcacaacctg
    24961 cataatatct gcacaataat ttttctcgaa ctcctttacc tgaatacatt cgtcactgga
    25021 aatcagataa ggaccgacat cgtacaaata agagggggaa accgagtcgg cgtaagctgt
    25081 gcgcaaacca accaacagct gttcctccgt gggatttagc gtgttgataa gatcaattgc
    25141 cacgctacgc cgatattcag caatatttgc caccgcaaaa gcagtatcaa taacggactg
    25201 tttcaggcta tacacccgtt cctttaccat ttgagtgtag atcccgcagg caactgctga
    25261 aatcatgata aaaatcagca gactgactgt aagtacgaac ttattgattc tggacatacg
    25321 taaactactc ttttactaat tttccacttt tatcccaggc ggagaatggc atgggtttct
    25381 ggtacagata cccctgagcc tgaacacatc ctaatttaat taattcgcgc gcaacgtctt
    25441 cgctctctac gccttccgca atcaccggga tccggttata gcgtgccaga tcgaaaataa
    25501 tgcgcacaat ttttcccgca gtaccgaaaa tatcgccggt aaattgcgag tccaccttta
    25561 tgctgtctgg attaatttca taaaaacgtt tcaggtttga caaaccggtg ccaaagtcat
    25621 caatcgatat ccgtacccct tgtttacgca gttgttcaat attgagataa aaatgttcat
    25681 taattcgctc gatcacggtt tcagtgatct caacacagag gctgttcgcc ttcaggccat
    25741 tttctttcat gatggtggta aaacgctcat gaaagtgcgg ttcgtttaac tgccagggcg
    25801 aaacattaat atgcagtaag aaatcatcct tcagcgacgc acgctgacta aaatgtctga
    25861 attcacccat tgccgtttta ataatctgat aacccagctc attgataaac ccgatatctt
    25921 cagcaatggt aatgaacttc agcggtgaaa tgatcccccg ctccgcagaa acccaacggc
    25981 acaacgcctc cgcgcccaga atggcgcggg tgttaatatc aacgatcggt tgatagacga
    26041 ggaatagttc gccaatttcc attgcatggc gaatattacg cgcaatccag atattctctt
    26101 ctatttcgtt gagtttcgcg tcatcacaaa aaataaactc ggagaacata tggtcacgga
    26161 tactggaaat cgccagtttc agtttttctg cgtaactcca ggcgttttcc ccctgcaaag
    26221 gttcacaaat ggcagactta tggatgcgca gattgcggta gccaaagctg ctgtcaaaat
    26281 cgtttaccat cgctatttta cgggtaaatg tctgtacatt ttcccgacac agaagtaaat
    26341 agagatcggc attgaatgtc acgatatcgc agcattgcgc atatttctcg gcaattcgct
    26401 gacacaattt cctgacgatt ttattgcgtt catcaacacc aaacaccgct tccacggctt
    26461 ccagaccaca cagtttgatg atccccacag ccagatactg attattgttg gactcaataa
    26521 tcgccatatt tttaataaaa ccgctttcct gatcgtaaga gatcagtgag agcaatttat
    26581 ttgaccgttc aatttcggca tctttcattt ccgcgatcga atttttgatg ctgataatct
    26641 ctttcgcatc gccttcgcga attttcagag cctcgatttg atccgttgag tcgggcaaaa
    26701 acgccagcgt attaaccagc tcggtcattt cagaaaattg ccgacggaac acacgagtgg
    26761 tcatgaaata taccaccagc aacacgataa taatgaaagc aaacggtaga taaaaaagtt
    26821 tatctgatgc gttcaataaa ttaccgccag gataataaaa atagatttcg aacaatgcat
    26881 tatttggcac tgctgatgtc tttgcaatat actccgtatc gacagaatca ataatattat
    26941 aattaccttc gctattataa ataatcagtt tttcacgagc gagttgattc ttggcaatga
    27001 tctcatgacc ttgtttcaaa aatagaactt catcattagc gctgatttga tcaagatact
    27061 cgttcagata ccgccagttt aaatcgacat gaattttatc taccgctaac aaatcgtgat
    27121 ctttgccgtt gatacgtagc gtaaaagaga tacatttttt acccggacga tcaaagcaat
    27181 tgtatgccgg attccagctg ttgttagagt catctgaagc caccgaccac ggtcgtttcg
    27241 atgggttaaa atcggcaaaa ataacccgcc ctgactcgtc gacaagcggt ccatttactt
    27301 gttcctgatg ataaacaacg attttatcat tcgccctccg cgaaaagaga taatatttat
    27361 tatcatcaag taccagtccc accgagtcta aaaatggcat catttccatt ttcagaatca
    27421 cggcgcgttg aatctcctct gtatttttta ataaatacag tgagtccttt aatgtcaggg
    27481 ccgaacgttc aatatcttta aagaaatagc caacgttttt ctgaataagt tcaaagcggc
//
LOCUS       DAJOWB010000056        27368 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJOWB010000056 DAJOWB010000000
VERSION     DAJOWB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.056"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            154..1374
                     /locus_tag="OHO85_002545"
     CDS             154..1374
                     /locus_tag="OHO85_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCQ2671905.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YADHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1347..1976
                     /locus_tag="OHO85_002546"
     CDS             1347..1976
                     /locus_tag="OHO85_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCQ2671906.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRINAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1977..3137)
                     /gene="ybdL"
                     /locus_tag="OHO85_002547"
     CDS             complement(1977..3137)
                     /gene="ybdL"
                     /locus_tag="OHO85_002547"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCQ2671907.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3246..4334
                     /gene="hcxA"
                     /locus_tag="OHO85_002548"
     CDS             3246..4334
                     /gene="hcxA"
                     /locus_tag="OHO85_002548"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCQ2671908.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4344..4541)
                     /locus_tag="OHO85_002549"
     CDS             complement(4344..4541)
                     /locus_tag="OHO85_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCQ2671909.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4723..6828)
                     /gene="cstA"
                     /locus_tag="OHO85_002550"
     CDS             complement(4723..6828)
                     /gene="cstA"
                     /locus_tag="OHO85_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCQ2671910.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7009..7422)
                     /gene="entH"
                     /locus_tag="OHO85_002551"
     CDS             complement(7009..7422)
                     /gene="entH"
                     /locus_tag="OHO85_002551"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCQ2671911.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7425..8171)
                     /gene="entA"
                     /locus_tag="OHO85_002552"
     CDS             complement(7425..8171)
                     /gene="entA"
                     /locus_tag="OHO85_002552"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCQ2671912.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8171..9028)
                     /gene="entB"
                     /locus_tag="OHO85_002553"
     CDS             complement(8171..9028)
                     /gene="entB"
                     /locus_tag="OHO85_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCQ2671913.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9042..10652)
                     /gene="entE"
                     /locus_tag="OHO85_002554"
     CDS             complement(9042..10652)
                     /gene="entE"
                     /locus_tag="OHO85_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCQ2671914.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10662..11837)
                     /gene="entC"
                     /locus_tag="OHO85_002555"
     CDS             complement(10662..11837)
                     /gene="entC"
                     /locus_tag="OHO85_002555"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCQ2671915.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12212..13168
                     /gene="fepB"
                     /locus_tag="OHO85_002556"
     CDS             12212..13168
                     /gene="fepB"
                     /locus_tag="OHO85_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCQ2671916.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(13172..14422)
                     /gene="entS"
                     /locus_tag="OHO85_002557"
     CDS             complement(13172..14422)
                     /gene="entS"
                     /locus_tag="OHO85_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCQ2671917.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14533..15537
                     /gene="fepD"
                     /locus_tag="OHO85_002558"
     CDS             14533..15537
                     /gene="fepD"
                     /locus_tag="OHO85_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCQ2671918.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15534..16526
                     /gene="fepG"
                     /locus_tag="OHO85_002559"
     CDS             15534..16526
                     /gene="fepG"
                     /locus_tag="OHO85_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCQ2671919.1"
                     /translation="MIYVSRRLIITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16523..17338
                     /gene="fepC"
                     /locus_tag="OHO85_002560"
     CDS             16523..17338
                     /gene="fepC"
                     /locus_tag="OHO85_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCQ2671920.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17335..18468)
                     /gene="fepE"
                     /locus_tag="OHO85_002561"
     CDS             complement(17335..18468)
                     /gene="fepE"
                     /locus_tag="OHO85_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCQ2671921.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISTLVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDLDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKAHVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGGVLLRYAMASRKQDAMMADHLV"
     gene            complement(18684..22565)
                     /gene="entF"
                     /locus_tag="OHO85_002562"
     CDS             complement(18684..22565)
                     /gene="entF"
                     /locus_tag="OHO85_002562"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCQ2671922.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSELPSAWSVAHYVELTGEVDSPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPDVQAQTHTLATGPVNDLELALFPDVHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVTRWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22562..22780)
                     /gene="ybdZ"
                     /locus_tag="OHO85_002563"
     CDS             complement(22562..22780)
                     /gene="ybdZ"
                     /locus_tag="OHO85_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCQ2671923.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEAHWRTLTPTNFTQLQEAQ"
     gene            complement(22783..23985)
                     /gene="fes"
                     /locus_tag="OHO85_002564"
     CDS             complement(22783..23985)
                     /gene="fes"
                     /locus_tag="OHO85_002564"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCQ2671924.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     PIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRGDRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24228..26468
                     /gene="fepA"
                     /locus_tag="OHO85_002565"
     CDS             24228..26468
                     /gene="fepA"
                     /locus_tag="OHO85_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCQ2671925.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPLDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26643..27263
                     /gene="entD"
                     /locus_tag="OHO85_002566"
     CDS             26643..27263
                     /gene="entD"
                     /locus_tag="OHO85_002566"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCQ2671926.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
BASE COUNT         6483 a         7672 c         7138 g         6075 t
ORIGIN      
        1 tttttgatat ataactacag acagctacta taactttcat ctatttattc acagcgcgaa
       61 tgaaaaataa aacactctgc tttacagtgt ttactttata attaacagtc ttatctcact
      121 gttcaccctg taacgttcag agagcgtctt cagatgtcta tttataaaat tcctcttccg
      181 ctcaatattc tggaagctgc acgagagcgt attacctgga cactcaacac cctaccccgc
      241 gtttgtgtct cattttctgg cggcaaagac tctggtttaa tgctgcatct gacagccgaa
      301 cttgcccgac aaatgggcaa aaaaatctgc gttttgttta tcgactggga ggcgcaattc
      361 tcttgcacca ttaactatgt tcagtccctg cgcgagttgt acaccgatgt catcgaagag
      421 ttttactggg ttgcgctccc gcttacgacg caaaattccc tttcacaata ccaacccgaa
      481 tggcagtgct gggaacctga tgtcgaatgg gtgcgtcagc ccccgcaaga tgcgataacc
      541 gatcctgact ttttctgctt ttaccagcca ggcatgacct tcgaacaatt tgtacgtgag
      601 tttgccgaat ggttttcaca aaaacgtccg gcggcgatga tgatcggcat ccgtgcggat
      661 gagtcctaca accgttttgt cgccatcgcc agtttaaata aacaacgttt tgccgacgat
      721 aaaccctgga ctacggccgc accaggcggt catagctggt acatttaccc catttacgac
      781 tggaaagtgg ctgatatctg gacctggtat gcagatcatc aaagtctctg caacccactg
      841 tataacctaa tgtatcaggc aggcgttcct ctgcgtcata tgcgaatttg cgaacctttt
      901 ggcccggagc aacgacaagg attatggctc tatcacgtta tcgaaccgga tcgctgggct
      961 gctatgtgcg cacgagtcag cggggtaaaa agtggcggca tttacgccgg acatgacaat
     1021 catttctatg ggcatcggaa aatcctcaag ccagaacatt tagactggca agaatatgca
     1081 ttattgctgc tcaatagcat gccggaaaaa acagctgagc attaccgcaa taaaatcgcc
     1141 atttatttgc actggtatca gaaaaaaggc atcgaggttc cacaaaccca gcaaggggac
     1201 attggcgcga aagatatccc ctcctggcgg cggatatgca aagttttact caataacgat
     1261 tactggtgtc gggcattatc atttagtcct acgaaagcga agaactatca gcgttataac
     1321 gaacggataa aaggaaaacg tcaggaatgg gggatactat gcaacaacga ttaacgcagg
     1381 atttaacgca atttctcgcc agcttgccgg aagatgatcg catcaacgcg attaatgaaa
     1441 tccgcatggc gatccatcag gttagcccct ttcgtgaaga gccagtggat tgcgttctgt
     1501 gggttaaaaa cagccaactt atgcctaatg attacaaccc aaacaacgtg gcaccacccg
     1561 aaaaaaaact gctgaaaaaa tccattgaaa tagatggttt tacccaaccc attgtggtca
     1621 cgcatacgga taaaaatgcc ctggaaatcg tggatggttt tcaccgccat gaaattggta
     1681 aaggctcatc atcattaaaa ttacgcttaa aaggttattt gccggtgacc tgtctggaag
     1741 gtacgcgtaa tcaacgcatc gccgcgacta ttcgtcataa tcgcgctcgt ggacgccatc
     1801 aaatcaccgc catgtcggaa atcgtccgtg aacttagcca gttgggatgg gacgataata
     1861 aaattggcaa agagctggga atggacagcg acgaagtgct gcgcctgaag caaattaacg
     1921 gtctgcaaga gctgtttgcc gaccgtcaat attcccgcgc ctggactgtc aaataactac
     1981 agctggcgca gtcgttcagc tgctgccagc aacgtcgatt ccttcttggc aaaacagaga
     2041 cgaatcagtt tatgagggaa gggatcggcg caaaacaccg atagcggaat cgccgctacg
     2101 ccgtgctcct gcgtcagcca ctggcaaaac tcaacatcat ccagggtaga tacggcgctg
     2161 taatccacca gcaaaaagta tgtaccttca cacggtaaaa tctccagccg gctttcattt
     2221 aaagcattca ccagaatatc gcgcttctgg cgataaaagt ccggtaacgc aagataatgc
     2281 tcaggttctg cacgtagcat atcagcaagc gccagctgtg ccggggtatt caccgaaaag
     2341 gtcagatact gatgtacctt gcgaatttcg gcgctgatgg gcgctggcgc aacacaataa
     2401 cccactttcc agccggtcat atgataggtc ttgccaaatg aagaaaccgc cactgcccgc
     2461 tcacgcagct gcggatgcgc cagcacactg gcatggccct gttgtgaaaa gttgatgtgc
     2521 tcgtagactt catcgctaat gacaaaaatc tcgtgcccgg cgatcgcctg ccacaaagcg
     2581 gcgaaatcag cctgctgcca gacagttgca ctggggttat gcggagtgtt gaggatcacc
     2641 agtctggtgc gctcgcttaa caatgcggca aattcctgcc agtcaacgcg aaaatgcggt
     2701 ggttgcagtg ccatacgctt cactattccc ccagaaagcg cgatggcggg ggagtaactg
     2761 tcatagctgg gatcaaaaca aatcacttca tcgccattgc gcaccagtgc ggtaatcgcc
     2821 gcgtataacg cttccgtcgc ccctgccgtt acggtgatat cgctatcggc atctggttga
     2881 tagccataca aacgttccgt tttctgagca atcgcctcgc gcaaggcctg cacgccggtc
     2941 atgggcgcgt attggtttgc cccctgggca acgtggtacg ccagccgctc ctgtaaatag
     3001 cgcggaccat caaaatcagg aaagccttgc gacaggttaa tcgcctggtg ttgctgcgcc
     3061 agcgcgctca tctgggtgaa aatagtggtg ccaagttgtg gaagtttgct ttgtggaatc
     3121 agagggttat ttgtcatttt tattgtacct gacgaagttg cgtgttgaag gcactataac
     3181 acgatgctag tatttggcaa tcaagacgtt tagatgtcta aataaaacaa taaggacaac
     3241 acaacatgcc tcacaatcct atccgcgtgg tcgtcggccc ggctaactac ttttcacatc
     3301 caggaagttt caatcacctg cacgattttt tcactgatga acaactttct cgcgcggtgt
     3361 ggatctacgg caaacgcgcc attgctgcgg cgcaaaccaa acttccgcca gcgtttggac
     3421 ttccaggggc aaagcatatt ttgtttcgtg gtcattgcag cgaaagcgat gtacagcaac
     3481 tggcggctga gtccggtgac gatcgcagcg tggtgattgg cgtcggcggc ggcgcactgc
     3541 tcgacaccgc gaaagccctc gcccgccgtc tcggtctgcc atttgttgcc gctccgacga
     3601 tcgccgccac ctgcgcagcc tggacaccgc tctccgtctg gtataacgat gccggacagg
     3661 cgctgcatta tgagattttc gacgacgcca attttatggt gctggtggaa ccggagatta
     3721 tcctcaatgc accgcaacaa tatctgctgg cggggatcgg tgacacgctg gcgaaatggt
     3781 atgaagcggt ggtgctggct ccgcaaccag aaacgttgcc gctaaccgtg cggctgggga
     3841 tcaataatgc gcaggccatt cgcgacgtct tgttaaacag tagcgaacag gcacttgccg
     3901 atcagcaaaa tcagcagtta acgcaatcat tttgcgatgt ggtggatgct attattgctg
     3961 gtggtgggat ggtcggtggt ctgggcgatc gttttacgcg tgtggcggcg gctcatgccg
     4021 tgcataacgg tctgactgtc ctgccgcaaa ccgagaagtt tcttcacggc accaaagtcg
     4081 cttacggaat tctggtgcaa agcgccttgc tgggtcagga tgatgtgctg gcgcaattaa
     4141 ccggggcgta tcagcgtttt catctgccga ctacgctggc ggagctggaa gtggatatca
     4201 ataatcaggc ggagatcgac aaagtgattg cccacacctt gcgcccggtg gagtccattc
     4261 attacctgcc agtcacgctg acaccagata cgttgcgtgc agcgttcgaa aaagtggaat
     4321 cgtttaaagc ctgacgtgca aatttagcag cagcgcgcgc cgccttttcc accgtagcgc
     4381 gcgtcctgcc gctcacggaa aaactcctcg taggtcatcg gcgtttgatc gggatggtta
     4441 acccgcatat gttcgacata gttgtcgtaa tcaggcatac caatcatcag cttcgccgcc
     4501 tgacctaaat attttccggc ttttgccagt gaatcaaaca tcttggcctc cgctaaccat
     4561 gttgctgaaa gagtgccgga tgcggcgtga acgccttatc cggcctacaa atgctatcaa
     4621 cgcgctcggt cagtcccctc gccccatcgg ggagagggtt agggtgagga ggaaccctcg
     4681 gcactatgcc aacattcgcc aacatccccc ctcactctga ctttagtgtg cgccttttgc
     4741 ctgcgccacg atctcctcga cattttccgg cattggctca tacggcgttt ctttcgccgt
     4801 tggcttcgga tctttcaatg ccgccagtgc cgtcttaatc gagaacagcg ccagaaccac
     4861 cacgaccacc ataaagaaga tggttaaccc ggcatccaga cggttgttga acaccagttg
     4921 cgccagctgt gactcagtat actgcgacgg aatattgccg ctgtcgatca ttgcctggaa
     4981 cttattagca atggccagga agccgacttt cgcatccggg ctaaacgctt tctgccagcc
     5041 tgcggtcagg gtacaaatca gcagccaggc cgttggtacc agcgccaccc aggcgtaacg
     5101 ttgacgtttc atcttgaaca acaccacggc acagagcatc agcgccatcc ctgccagcat
     5161 ctggttggca ataccaaaca gcggccacag agtgttaatg ccgcccagcg gatcgaccac
     5221 gccctgatgg aggaagtagc cccacgccag tacgcacagc gctgttgcca gcaggttagc
     5281 aggcagtgaa tcggtccgct tcaggcccgg agaaaccacg cccagcagat cctgcaacat
     5341 aaagcgcgca gcacgcgtac ccgcatctac cgccgtcaga ataaacagag cttcaaacag
     5401 aatggcgaag tgataccaga acgccacatc catcatacca cccagcgcac cgtgcaggat
     5461 gtaagccatc cccaccgcca gcgtcggcgc accgcccgca cgggaaatga tcgactgttc
     5521 acccacttcg ctggcaatct ggttgagcgt atctggtgta atgctaaagc cccagctact
     5581 caccacctgc gcggcagaag cgactacatc cgccgtccct gccggagcca gcaccgccat
     5641 tgggctgttc atggcaaaat acacgcccgg atcgatgata caggcagaaa ccagcgccat
     5701 aatcgccacg aaggattcca tcaacatccc accgtagccg ataaagcacg cctgcccttc
     5761 gttcgccagc atcttcggcg tggtgccgga agagatcagc gcatggaagc cagacaccgc
     5821 accacaggcg atggtgataa acaggaacgg gaacaggtta ccggtccata ccgggccagt
     5881 gccatcgaca aatttggtca gcgcaggcat ggtcagcgtc gggcgcataa tcaaaatgcc
     5941 taccgccaga ccgacgatag tcccgatttt caggaaggtc gagagatagt cacgcggggc
     6001 cagcagcaac cacaccggca gcaccgccgc cacaaaaccg tagcccacca gcatccaggt
     6061 cagctgcacg ccggtaaagt caaagtacgg tgcccaggtc ggactttctg ccacccagcc
     6121 gccagagata atggcgaaaa tcaggaacac caggccaatc actgacactt cgccaatgcg
     6181 gcccggacgc agatagcgca ggtagattcc cataaaaatc gccagcggaa tggtgaacgc
     6241 aacggtatac gttccccacg ggctatgagt cagggctttc accacgatca tcgccagtac
     6301 tgcaaggata atgaccatga tcataaagca ggctaccagc gcaatcaccc cggcagttgg
     6361 ccccatctct tctttgacca gctcacccag cgatcgaccg tcacggcgcg tagaaacaaa
     6421 cagcaccatg aaatcctgca ccgcaccagc gagaaccacc ccggcgagca gccagatcat
     6481 ccctggcagg taacccattt gcgccgccag taccggccca accaacggac ctgctccggc
     6541 aatggccgca aaatggtgac cgaacagcac tttcttgtcc gtcggcacat agtccagccc
     6601 gtcgttatgg cgcaccgctg gcgtcatacg cgtcgggtca accgccagca catttttggc
     6661 gatatacagc ccataaaaac ggtaagcgat cagatagata cagaccgacg ccaccacaat
     6721 ccacagcgcg ttaatctgtt ccccacgatt taaagcaatg tatcccagag caaatgctcc
     6781 cattacagag agcactgtcc agacgaggta tttccctgat ttgttcatag ttgttatccg
     6841 tgtgcgtgtc catagagatg ttacattttg tttctataac atctttgtac gatttaacaa
     6901 cacggtaacc atgtaaatcg gagttgaaac caatatttaa cttaacaatg ttaactcgat
     6961 cactccgtta accgagtttt cctgcaatct catttaattc tgtcgggctc atcccaaaac
     7021 tgccgtaccc agccgacaag tgcagcaacg ccgcccctgt tcatcgaaaa cgacgatttc
     7081 ccagctctga ttttgccgcc caagatgcag cggctggcag acgccgcgta ccttgccttc
     7141 agacaccggg cgatgatggg tggcgttaag ttccgtaccc accacacact gcccgtcacg
     7201 ggtcatcata aatccggcca tcgatcccag cgtttcagcc agcgccgccg acgcgccgcc
     7261 atgcagcagg ccaaacggct gatgagtacg ggtatcaacc ggcatttcgg cttccagcac
     7321 atcatctccc agacgggtat acacaattcc cagatgcgcc accattgtgt tatcgctggt
     7381 ggcgttcagt tcgtcgagcg ttaaatggcg tttccagatc atgcttatgc ccccagcgtt
     7441 gagccgccat cgaccacaat atcctgtagg gtgatatggc tggcgaggtc agaggcgagg
     7501 aacaaaatcg tgttggcgat ctcttgtgga cgggcgattt tccccagcgg aatgccgagt
     7561 ttaaactgct cgccaaagcc gcgaatacgc tgttcttcgg cgtcatcgct cacccacagc
     7621 gtgcgttgca tatcggtgtc ggtggagcca ggcgaaacca cattacagcg cacgccgcta
     7681 cccgccagtt ccagcccgac gctcaacgcc aggcttttca gcgccgcttt cgatgcgcca
     7741 taagcactca taccaatacg cggcgtgtgt gcggcgtcgg acgccacagt gacaatcgcc
     7801 ccgccccgct gacggcgaaa ctggttcatg gtttgctgga acaggttaaa cgcaccgccg
     7861 acgttaaccg caaaagtctg ctgccagtcc tctttactga gctgatcggt cgcgcccatg
     7921 cgcaaaattc ccgccgcatt gatcagcacg tccagtcgct ccgtttcagc taacagtcgc
     7981 tgacatacct gcgccacctg cccggcgtcg gcaacatcca ttacttcggt cgcaaaggga
     8041 tattgctcct gagtgaacgc ttgatcaaaa cctgtaactt tcgctcccgc ctcaacaaac
     8101 gccagcgccg tggcgtagcc gataccttta cctgcgccgg ttacccagac atttttaccg
     8161 ctgaaatcca ttatttcacc tcgcgggaga gtagcttcca ccaggcgtcg atggtcgggt
     8221 ttttcgccag catgacaaag tcgatatcac catgcacttt gcgccagcgc gccgccagcg
     8281 ccatcatgcg caccgaatcc agaccgtagt cgatcaggtt gtcgtcatcg aacggttcat
     8341 cggactcgtc cagcaacggc aggataacct cacgcagcgc cgctttgctg gcggggacag
     8401 gtgctggcag taattcttca gtcatcacca cccggccaga ccgcccggcc acatatttca
     8461 gcgacatcaa atgctcgtca cggctgaaat cggccagcgc atccgccacc ataaacggtt
     8521 taatatcgcg cataaatgcg tcggttgcgg tggtcataca gccaatgtgg gcatataccc
     8581 cggtaataat cagctggtta cgtccactct ctttgagcat ttgctccagc ggagaacgat
     8641 gaaacgcgct gtagcgccac ttcaccagca ccgtgtcgtc ggcatctggc gtcaggcgat
     8701 ccaccacctt ttgctgttcc ggcgaacggg tcaggcccgg cccccacata tcattcaaca
     8761 gcgcccgatc ttcatcgctc tgctctttcg gctgggcggt gtaataaacc gggatattgt
     8821 gctgtttgca gtagtcgcgc agcgcagcaa tattcgcgat cacctgctcc atcatcgggc
     8881 agttctcgcc ccagaagctg acaaaatagt cctgcatatc atggattaac aacgcggcac
     8941 gttgcggttc aaaggcccag tcaactttat tctgcggaat atcgtgagac tccggcagtg
     9001 cgtaagcctg taattttgga atagccatag tgttctctcc ttcaggctga tgcgcgtgac
     9061 gccagccact gacgtaattg ttttttatcg actttcccga ccgccgtcag cggaagtgaa
     9121 tccacacact ccacgcgatc cggtaattta aattcggcaa taccctgttc acgcaggaaa
     9181 cgacgcacct gcaccgcgcg cagcggctct tttaccacca gataagcgca gcttttttcg
     9241 cccatcagct catcttccat gctcaccagt gcggcataga tcactgccgg atggcgcagc
     9301 agcaggtttt cgatctcttc ggcagcgatc ttctcgccgc cacggttgat ctgatctttc
     9361 tcgcgcccct gcacggtgat gtaaccctct ggatcaatag agatcagatc gccggaacag
     9421 taaaaaccgt tggcatcaaa ggcgctggca ttgtgctgcg gacttttgta ataaccgcgg
     9481 aaggtgtacg gcccgcgcgt catcaggcgt ccgacttccc cttgcggcag tggatttcct
     9541 tcggcatcgg caacccacac ttcatcgtcc ggacacattg ggtaaccctg ggtatggata
     9601 attttctccg cgctatcatc aagacgggtg tagttcacca gcccttccgc catgccaaac
     9661 acctgctgca actgacagcc aatctcagcg ggaatacgcg ccgcaagggt ggcagaaaga
     9721 cgtgcgccgc cgacctgtaa cagtttcagc gaggcaagct gcgcccggct ttcgccttcg
     9781 gtcagcgcct gcaaccacag gctgactgcg ggtggcacca gcgcggtaac gttaacctga
     9841 tgtttttcaa tcaatgggaa acagagcgtg gcgctgggat cggccgccag aacaaccgtt
     9901 cctccggcaa gaaagacgcc cagcgatccc ggcgaactca tggcgtagtt atgagccgcc
     9961 gggatcgcgc acaggtagcg tgtctgttgt gtgaactgac aaatctcgac gctacgacgc
    10021 acgctgtagt agtagtcgtt atgagtgcgc gggatcagtt tcggtgtgcc ggtggtgccg
    10081 ccggaaagct ggaaataggc cacttcatca gcaggtgatg gcgtggcagt aaaatcctca
    10141 gccggatggt taatcgcatc ctgcaagtta tgctcaccgc tgtcgttgtg cagttgcacc
    10201 acgcgaatgg aggaatgttc tgtgacgaaa gtattgagga aatcatcccc gctaaacagc
    10261 gcatgttggc gatcggcaat cagcaatgcg ggttcaatct ggctggcata ggcgttcagt
    10321 tcactacgct gatggctgaa cagcgccagc accggcgcaa cgcccagttt cagcagcgcg
    10381 aaaaaggtaa tatacaattc agcgacgtta cccagttgta ccagcgcggt ttcaccaggt
    10441 ttaatgccct gacggcgtaa actacacgcg aggttatccg ccgcctgatt cagctcccgg
    10501 taactcaact gtcgctcgcc gtcgataacc gcgatgctgt cactcgcagc gtggcgagtc
    10561 agaatgtcgg tcagcggcaa atcctgccag tagccttttt cccgatagcg acgggcaaac
    10621 tcttccggcc agcgggtgaa tggaatgctc atcctcgctc cttaatgcaa tccaaaaacg
    10681 ttcaacatgg tagaaagttt gacgcctgtt tcgcgccact cacccaacgg tgacgacgca
    10741 ggcacaatcc ccgctccggc aaacagacgc acctgatttt cccgcagctt cgcgcagcgg
    10801 atggtcacca cccattcgcc gttaccttcg ctgtcacacc aacccacaat gccgccaaac
    10861 agttcgcggt cgaatggctc cagttcagca ataacctggg tcgcggcctg atgcgggaaa
    10921 ccgctcagcg cgggggttgg atgcagcaga caggccagag tcagtgcgtt ttcttgcgaa
    10981 ttcgctttac cttcaaaggg agttgcgaga tgccacagcg tcggcgtggt aatcaattgt
    11041 ggagaggagg gaacgtgtaa ctcactactg cgttcgcgca gtacctcttt catcgcctga
    11101 gtcaccagtt catgttcatg gcgatctttt tctgacgcca gcagacgatt acccgcttcg
    11161 cgatcgagca cttcatccgg ctgacgacgc gcggaaccgg ctaacggaat ggagctaaaa
    11221 cgctcgccgt ctttacgtag cagcagttcc gggctggccc ccagcaggac gccaccatca
    11281 gccagcggga catggaagtt gtaactaacc gggttttgcg caatcaaccg ttccagcaat
    11341 acgccactat caatggcggc gtcagtggtg atatcaatca atcgtgacaa caccactttg
    11401 tcgacctgcg gcgtggcggt aagtgcggcg gcgcgagcaa ccatctgttc aaacgtggtt
    11461 tgttcaggaa ttgcctggcg ttccaccacg ttcagcgact ggctgcgggt aaaacggcgg
    11521 gctgaggtct gtttttcctg gcgggagaac gactgccagg attcgggaat atacagcgac
    11581 gaaggctgac gtggatcgaa gggaatcgcc ccgaccatca ccggattttt gatgccctgc
    11641 gctttggcat cggcaaacag cgcggcgagt ttttgctgga agggactgtc gggcgaatcc
    11701 ccgttcacag ccggttcatc gaagcgggcg aaacatcctg acgtcgtaaa actgcggtac
    11761 ggcgacataa agaaaaagcg attgggcgca agtgttgcca tggtctgctg tacttcctca
    11821 gccagtgacg tatccatatc atcctccaca aaatgataaa ggctttaata atgattatca
    11881 tttatatttt cggtcgctaa cctaaaagca aacgcgcact atgtcaactc ttgaggtaac
    11941 gcaatctgca actcggggtt gcatctggtt gtggttacaa tgaaaatgag aagcattatt
    12001 gatggattcg cataagcgca atgtgatgtc ctgcgccgtt ctgccccctc tcccttccag
    12061 ggtgagggct ggggtgaggg ttaatgttcg caccagtgct ggctgttccc ctcaccctaa
    12121 ccctctcccc aaaggggcga ggggacggat tgtgcgcttt gtcgaatttg tcattacgcc
    12181 cttaacctta ttaataacag gaagctgatt tgtgagactc gccccgctct accgcaacgc
    12241 ccttctatta acaggacttt tgctttcagg aatagccgca gttcaggccg ctgactggcc
    12301 gcgtcagatt actgacagcc gtggcacaca tacactggaa agccagccgc agcgtattgt
    12361 ttccaccagc gtcaccctga ccggctcact gctggcgatt gatgctccgg tgatcgccag
    12421 cggcgcgacc acgccgaata accgcgtcgc ggatgaccag ggctttttac gccagtggag
    12481 caaggtggcg aaagaacgca aactgcaacg gctctatatc ggcgaaccga gcgccgaagc
    12541 cgttgccgcg caaatgccgg atctgatttt aattagcgca accggcgggg attcggcgct
    12601 ggcactgtat gatcagcttt ccaccatcgc cccgacatta atcatcaatt acgacgacaa
    12661 aagctggcag tcgctgttaa cgcagcttgg cgaaattacc gggcatgaga aacaagcggc
    12721 agagcggatt gcacagtttg ataagcaact ggcagcggcg aaagagcaaa tcaaattacc
    12781 gccgcagccg gtcactgcca ttgtctatac cgccgctgca cacagtgcca atctctggac
    12841 gccggaatca gcacaagggc agatgctgga acaactcggc tttacgctgg cgaagttgcc
    12901 cgcagggtta aacgccagcc aaagccaggg caaacgccat gacatcattc agcttggtgg
    12961 ggaaaatctg gctgctgggt taaatggcga gtcactattc ctgttcgccg gtgatcagaa
    13021 agatgccgat gcgatttatg ccaatccact gctcgcgcac cttcccgcag tacaaaacaa
    13081 gcaggtttat gcgctgggaa ccgagacgtt ccgtctggat tactacagcg ccacgcaagt
    13141 gctggaacga cttaaggcgc tgttttaagc attaactgtc agacgctgtc acctgcggcg
    13201 gcgtctggcg aaaacgtcgc aactccacca gcaccagcaa taacaacacg ccgataatca
    13261 acaaaccaaa accgcttgcg cttgcggaag caaccggtgt catcatcgcg cccaaaccac
    13321 ccagcagcgc cgcgcctatg gcatcgcccg tcacgttctg cgccgtccac aaaccgttaa
    13381 tccgccctaa catcgcttcc ggggtttgcg tttgcagcat tgtatattgc agcaacgaac
    13441 taaccgcgct cagccagccg aacagcgcca gacaaaccac gcctaaaatc cacatcggca
    13501 tcaggccaaa cagaccaatg gcgaggaacg atcccagcgt ggagagcagc atcaataacc
    13561 ctggtcgcgc actatgtgcc agcttcccgc tggttaacgc accaatagcc gcgccgagcg
    13621 ggatcgccgc gtagagaaaa ccaatctgtg ccgctgacat ctgccagttg tcagccagcg
    13681 ccggatacag tacccgcacc gcgctcgcca tcgtcaataa accacccagc agcgcaatcc
    13741 cgcccaccag cgggctggca agcagaaaac gaaatcctgc cagtaatgat ttcaacggat
    13801 gctcacgcgg ctgcggtggc ggtggcaacg ccggaaggct taacaacggt agcaaggtaa
    13861 taaacgtgcc cgccgccgcc agcccgtagt tccaggctac gccaccggtc gccagcaata
    13921 aaccgccaat catgggcgaa atcaccgacc ccagacgcac ggtcaacatg gtgatcgccc
    13981 cggcctgcat taagttttca cgccctacca gtgctggtgt cgccgccaat agcgccgtaa
    14041 cgccaagcga tgcgaaaaaa ccatcccata aaccaagtaa atagattgcc agcaatgacg
    14101 gctccggcag cagtgcatta aggcacagtc caatgaagcc aatgccacag gtgccgcgcg
    14161 ccagcaaaat cacttttttg cgctcatagc gatccgccag cacaccgcca accatcaggc
    14221 caacaaacat cgcgccgccg gtcagcgtca ccgaaagccc cacctgccag gtagaatgcg
    14281 tcatcatctg gatctgcacc ggcaccgcga cgccgagcaa acccagagac acaattgaga
    14341 taaaacgagc gaggaatact gcgcgaaacg ccgggtgcgt tttcaacagg ctgaggttaa
    14401 gcagccagga ttgtttattc attacaatgc cttgccatca aatattttaa atacctattc
    14461 ttaggctgca catgctaaca tatccaaata agatcgataa cgataattaa tttcattatc
    14521 atggaagttc gtatgtctgg ttctgttgcc gtgacacgcg ccattgccgt gcccggattg
    14581 ctgttattac tgattatcgc gacggcatta agcctgctca ttggggcaaa atcactcccc
    14641 gcttccgtag tgctggaggc cctctccggc acctgccaga gcgccgactg caccatcgtg
    14701 ctcgacgcgc gactgccgcg cacccttgcc ggtttacttg caggcggcgc gcttggcctt
    14761 gccggggcgt tgatgcaaac cctcacccga aatccacttg ccgaccccgg cttgcttggc
    14821 gtgaatgccg gagccagctt tgccattgtg ctgggtgcgg cgctgtttgg ttactcatcc
    14881 gcgcaggaac aactggcgat ggccttcgcc ggggcgctgg tggcctcatt gattgttgcc
    14941 tttaccggca gtcagggcgg cgggcagtta agtccggtgc gtttaaccct ggcgggcgtg
    15001 gcgctggcgg cggtgctgga agggctgacc agcggcatcg ccctgcttaa tcctgatgtc
    15061 tacgatcaac tgcgtttctg gcaagccggt tcgctggata ttcgcaatct acatacctta
    15121 aaagtggtgc tgatcccggt gctgatcgcc ggagcaaccg cgctattact gagccgcgcg
    15181 ctgaacagtt taagcctcgg cagcgacacc gcgacggcgc tgggcagtcg cgtggcgcgc
    15241 acacagttaa ttggtctgct ggcgattacc gtgctttgtg gtagtgcgac ggcggtagtt
    15301 ggcccgattg cctttatcgg cctgatgatg ccgcacatgg cgcgctggct ggtaggtgcc
    15361 gatcatcgct ggtcgctgcc cgtcacgcta cttgctaccc ctgccctgct gctgtttgcc
    15421 gatatcatcg ggcgggtgat tgtgcccggc gaactgcgcg tttctgtggt cagtgcgttt
    15481 attggtgcac cggtgctgat cttcctcgtg cgacgtaaaa cgcgaggtgg cgcatgattt
    15541 acgtctctcg ccgattaatc atcacctgtt tgctgttggt ttccgcctgt gtggttgcag
    15601 gtatctgggg attacgcagc ggtgccgtca cgctggaaac ctcgcaggta ttcgccgcgc
    15661 tgatgggcga tgcgccgcgc agtatgacga tggtggtcac cgaatggcgt ttaccacgcg
    15721 tgctgatggc gctgttgatt ggcgcggcac tgggcgtcag cggcgcgatt tttcagtcgc
    15781 tgatgcgtaa cccgctcggc agccctgacg taatgggctt taacaccggg gcgtggagcg
    15841 gcgtgctggt agcgatggtg ctgtttggtc aggacctgac ggctatcgcg ctggcagcaa
    15901 tggtgggcgg cattatcact tcgctgttgg tctggctgct cgcctggcgt aacggcatcg
    15961 acacctttcg gttgattatt atcggtatcg gcgttcgtgc catgctggtg gcctttaata
    16021 cctggctgtt gctgaaagcg tctttagaaa cggcgctaac agcgggtttg tggaatgcag
    16081 gatcgctcaa cggcctgacg tgggcaaaaa cctcgccttc cgcacccatc attatattga
    16141 tgctcattgc cgccgcctta ctggtacgcc ggatgcgctt gctggaaatg ggcgatgata
    16201 ccgcgtgtgc gctgggcgtc agcgtcgaac gttcgcgtct gttaatgatg ctggttgcag
    16261 tggtgcttac cgctgcggca acagcgcttg ccgggccgat ttcctttatt gctttagtcg
    16321 caccgcacat tgcccgacgc attagcggca ccgctcgctg ggggctaacc caggcggcgc
    16381 tatgcggggc gctgttactg ctggcggccg atctctgcgc ccaacaactg tttatgccgt
    16441 atcaacttcc ggttggcgtc gttaccgtca gcctcggcgg tatttacctt atcgtcttgt
    16501 taattcagga gtctcgcaaa aaatgaccga atcagtagcc cgtttgcgcg gcgaacagtt
    16561 aaccctggga tatggcaaat ataccgttgc ggaaaatctg actgtagaaa tacctgatgg
    16621 tcacttcacg gcaattatcg ggccaaatgg ctgcggtaaa tccacgttac tgcgtacctt
    16681 aagccgcctg atgacgcctg ctcatgggca tgtctggctg gatggcgagc acattcaaca
    16741 ttacgccagt aaagaggttg cacgccggat tggtctgttg gcgcaaaatg ctaccacgcc
    16801 gggcgatatc accgtgcagg agctggtggc gcgtggacgt tatccgcatc aaccgctgtt
    16861 tacccgctgg cgcaaagagg atgaagaagc ggtaacgaaa gcaatgcagg ccacgggaat
    16921 aactcatctg gcagatcaaa gcgtggatac cctttctggc ggacaacgcc agcgagcgtg
    16981 gatcgcgatg gtgctggccc aggaaacggc aattatgctg ctcgacgaac ccacgacctg
    17041 gctggatatc agtcatcaga ttgatttgct ggagttgtta agcgaactga accgcgaaaa
    17101 aggctatacc ctggcggcgg tgctgcacga tcttaatcag gcctgtcgtt acgccagcca
    17161 tttgattgca ttgcgggaag ggaaaattgt tgctcaggga gcgccgaagg agattgtcac
    17221 tgctgaactg attgagcgca tttatggtct gcgctgcatg atcattgacg atccagttgc
    17281 cggaacgccg cttgtggtgc cgctcggacg aacggcacct tcaaccgcaa atagttaaac
    17341 taagtggtct gccatcatgg catcctgttt tctggatgcc atcgcatagc gcaacagcac
    17401 gccaccacaa gccaccatcc cgccgatcaa cgcggaaagg atcacaataa tcgccttacc
    17461 cggaccgtct tttttcactg gcaatgacgg gcttaactga tatttaaacg gcgtaaaatt
    17521 cacatcgttg acatgtgctt ttgttaattg ctcgacaaga tactgccgat tacgtaattc
    17581 accgttcagt tccgcaacgt cagtgaccgc tttttctatt tccagtttgc gttcaatacc
    17641 gtctgcaccg agagaaatag aaaaatcgag gtcatcttta acggcctgac cattactgta
    17701 aacgggcttt ttaattcctg ccgcgttggc aatgtcgagt gaataattga ggcgctgaat
    17761 gtttgcatca agttgatttt tcgttttaat gcgatcctga gccagttttt ctttttcaaa
    17821 ctgggttttg atctccagtt tattacggac gttttctagc gactctttca ccaccaacgt
    17881 agagatataa tcgatatacc cgctcaaaac ggtctgcgcc tcttcactgg ttggcgcggt
    17941 aaaacttagc gtccaggagg tatacagtga cggttcatct tttttcttac tggcattgtc
    18001 atcaaccgct ttcatttttt cgctcaatgc gacaattgcg cgatgcaaat ccagttcgtc
    18061 gattttcgcc tcttttaatt ggtccatcac ataaggtgat gaacgcaggt actcttccag
    18121 caagctaacc gactgaaact tcttgataaa caggttaaat gcttctgtac gatcaatttt
    18181 gatatccaga tccagcactc gaagcttagt gaatgttttc tccagctctt gccactgaac
    18241 aggttctgga ggcgtgacaa ccgccgcgct ggtccatttt tgcggcagga tgaaagagat
    18301 cagcaagcct gcgcaggcaa acgcaaaaat gaccgccatg accgtttttt tggcccgcca
    18361 taaaactgcg attagattaa gtaaatcaat ttcattatta ctgggcgacg ccagaggata
    18421 ctcaggaaaa tgagcgtcac ttccctgttt aatattcagt gatgacatgg ggtataacct
    18481 gaaaatgagt ccaatagggg aaatctccac ggcgctcact ttaccagttg gtgataatta
    18541 tccgaagctg aagtttgtaa attccttcca ctgaatgcaa cattcactct atttaagaga
    18601 aatattcatc aaacctataa atcaaacaaa taaaaaaacc cataattatt ttctgtaatt
    18661 atgggtttat agatattatt aatttacctg tttagcgttg cgcgaataat cggcccaatt
    18721 tttacaaacg cccctggaga gataatatcc acatgcgcac aatcctgacg atagatatcc
    18781 agctccgcaa tccacggcga ccaggcacgt tcgggactca taccttcctg aagtgtgcgc
    18841 tcagcaacaa ataacgttgc ttttccatca aacggaacgc tatgggcggt ggttaacaag
    18901 cgcacagcat cagcgtagtt gccttcaatg gtggtaaaca actccgttga agtacttccc
    18961 tgctgtgccg ccaggaaggc ctcgcgttcg cggttaatct ccgccagcac ttccgggtcc
    19021 agaccattag cctctttttc ctgccagttt tgcgtctctg gcggccaggt atccagcaaa
    19081 ccaagaaatg ccacctgttc gccacgggca cgcagtcgcg ccgcaatacc ctgcgccagc
    19141 gtaccgccaa gggaataccc cagcaggtaa taagggccgt gcggttgttg ctcaagtaac
    19201 gttgccagat gcgcttcgca gacttcatcc aggtttgtcg ctgtctgcat gggtccatga
    19261 gggcgcggcg actgaatgcc aataatcgac cattgtggat cgagataacg cgagagcacg
    19321 ctaaactgcc aggcaaaacc ggacgccgga tggaaacaaa acagcgtcgg gccattacct
    19381 tcacgcaacg gcagaatggt ttcgaatccc atgcgccggg agctgtcctc ttcaccatca
    19441 ataatcgttg ccagtttggc gacggttgac gcgaccatca cctgccccgg cgtcacctga
    19501 cgagcaaact gtcgacttaa ctgcgctgct agtttcattg ccagtagcga atgaccgccc
    19561 agcgcaaaga aatcagcatc ggcatcctgc acgtcacaac ccagcaacga cgcgaatgcc
    19621 gcggcgataa tcgtttcact gcccgctttc ggtgcacgtc ccgacgcttg tgtcttcagt
    19681 tcaggcaacg gtaaggcttt gcgatccagc ttgccgttgg cgctaagcgg caactgcgga
    19741 agttgcagca gaacgaccgg caccatatgc ggcggcaatg tttcgcgaag ctgtgcctgt
    19801 aatgcgctgg tatccaacgg cagtcccgat tgcgacacca gatagcccac caactgacgc
    19861 gcatcaccac cggtggctgc cgcctggtta atcacacagg cgtgggtaac tgcctgttcg
    19921 acatccggca gcgcctgcat cacgcgatcg atttcgccca gttcgatacg ctgcccgcga
    19981 atttttagct gatcgtcact gcgcccgagg tactccaccg cgccgttatc cagccagcgg
    20041 gtgacgtctc cggtacgata catccgttca cctggggcaa aaggatcggc aataaaacgg
    20101 ctggcggtca gatcgggtcg tccaagatac ccctgcgcca gttggatgcc ggtgagatag
    20161 agatcacccg ccacacccgg cggcaccgga tgcatcatcg catcaagaat acgcagaccc
    20221 gtattccata ccggataacc aatcggcaca ctgctgccgc gcacctgtgc cagttcctcg
    20281 ccaaaagccg gataccagct gacatctacc gccgcttccg tcgggccata caggttatgc
    20341 aacggcgcgc cggttaactg ttgccattcg cggcataaat cagccggtaa ggcctcacca
    20401 ctacagaaaa cctgtttcaa cgtcgcgcaa ttctggcgag cggtttgcgg cgtcagcgat
    20461 gcaacaaatg ccgccagcat cgacggcaca aagtgcgtgg tcgttacgcc atattcggca
    20521 aagaattgct gcatagcgag cgggtcacgg tgcgcttccg gttcagccat caccagtttt
    20581 gcccctgcga taaacggcca gaaaaactcc cacaccgaga catcaaaact gcacggcgtt
    20641 ttttgggcaa cgacatcttc gcctgtaagc gggtagtgat tttgcatcca taacaggcgg
    20701 ttaacgatag ccgtctgccc gaccattacc cctttcggcc tgccggtgga gccagaggta
    20761 aagatgatat aagccgtgtg gtgcggttgt gaaagttgca gcggcgcact gccctggggg
    20821 gtaagcgggg cgttatagca aaggcttgtt aaattgggaa catcgctaaa gcgcggcagt
    20881 tgatcgtcgg tggtaattaa cagcgacgga cgcgcatctt ccagcatcat tttcaggcga
    20941 tcgtccggat agccggtatc cagcggtagc caggccgcgc cagcttcaac tattgcatgg
    21001 agtgccaggg tcaaaaagac cgagcgcggt agtgccaccg ccacgctgtc ccctggttta
    21061 acgccgcgct cacgcagcag attcgccagc gccaccacct gctcgcgcat ttcccgatag
    21121 ctgaacaggt aacgcgcatc tgccagcgcc ggagcatccg gtgtttttgc cgcttgttct
    21181 gccaccagcg cgctcagcgt ggtttctgga atctcaacct gagtggcgtt gatctgcgcc
    21241 agctgcgcat actcacctgg cagcataata tcgacatcgc cgcacaacag cgccggatcc
    21301 gcggcgaact gggcaatcag cattttcagg cgttcagcat gctggattaa cgttggctca
    21361 tcgtaacgct gtttattggc gaggatctca atactcaaat caccgtgtac atccgggaac
    21421 agggccagtt caaggtcatt aaccggaccg gttgccaggg tatgggtttg cgcctgaaca
    21481 tcaggaatat ccagttggta atcaaatacc ttgatattga gtaccggacc aaacagcggt
    21541 tcatcacctg ccgctcgccc gctgtcacgg acaatttgtt cggcatcgta acgttgatga
    21601 cgacgcattt ttttcagttg tgctgccagt cgggttgcca gttccggcag cgtttcttgc
    21661 gccgcaatgt gaatacccaa cggcaaaacg ttgagcacgg gtccggtagc cgtcagcgcc
    21721 gccgagccca gtcgacgcat aaagataaat ccggcggcgt agtccatacg attgcacaat
    21781 cgccccagcc acaaggctgc cagcgcaagg gctaaatcgg tacgctgcac acctgaaagt
    21841 tgcgtagcca gctggcggaa ttccccgtcg gtaaattcca gtttcaggcg cagaatatct
    21901 gccgaggcgc tgcgccccgg taaaggtgcc ggagaaagtg acgcgggcgg cggcagttga
    21961 cgacgctgtt ctgcccagaa tgccgcatca cgctgccagg cttcgctttc gcggtattgc
    22021 tggtactctt ccactacatc agcgaaaggc gtaaatggcg aagcaggcgt tggttcgcca
    22081 cgcagccatg tgcagtaaat attggcgatc tggcgggtaa tggccgggaa actgaagcca
    22141 tcgaccagca aatggtgata acgctgatac cagtaccagc ggttatccgc cacctgtatc
    22201 agctgatgaa agaccagtgg tttaccgcta tcgacgcgca gatcttgttg caaatccgcc
    22261 tgcattaatg cctgcgcagt accgtgcgga tcaatgttgg ttcgtaggtc gataatttct
    22321 ggcagttcga acgtcagcgc atcatcgacc cactgccaga cttcgccgtt atcttccgta
    22381 aaacgcatcc gcagcgtatc tgcttgcgct agtccggcaa ccaccgcgcg ggccagtaat
    22441 ggcgaatcaa cctctccggt taactcaacg taatgcgcca cgctccaggc ggagggtaat
    22501 tctgacagtt tttctgccat ccagatgccg ggctgtgcgg cgaccaaagg taaatgctgg
    22561 ctcattgtgc ctcctgcaac tgggtaaaat tcgtcggtgt cagagtacgc cagtgggctt
    22621 ccagccactg ctggcaggac gcctgtgact gcggctgaca cacaatgtcc cagcctgccg
    22681 gtaaggcgca ttgttgcggc cacagactga attgcccctg cgcattgcgc aaaatgtaaa
    22741 acgctccctg cggatcatcg aagggattac tgaatgccat attcaactcc tgtcatggaa
    22801 aagtggttgc cagaggtcga ttagcccctg catcaagcca ccgcgccaac aaagcgcatc
    22861 atgtccgccg tcaacctgac gccagaaaat ggattctttt atggggtgta attgcgcata
    22921 cagcgcctga ttggcccgca taatcatcgg ctcgcgaata cccgcttcca gcacaatgcg
    22981 cagaccttcg gcgctaactt caccagcttt aagcttttca agtaacacgc cctcttgctg
    23041 cccgccccga tgcggccacc agtacgatcc tgactggctt aatacacagc caaagcgttc
    23101 aggccagtgc agtccggcat acagcgcgga cagcccacca aaactctgcc cggcaaccac
    23161 ggtgcgatcg ccgcgatcgc taaaaggggc aatagctttc accaggggta ataactcttg
    23221 ctgtactgcc agccagaaat ccgcattaca cggcagttcg tgggcgcggt gcgtggtgtc
    23281 gatagcgtcg atcaacacat acacggcggg aggaagttgc tgacgatggg tcagcgaagt
    23341 cagcacaggc cagacgggca tactttgcgc ccaaaattcg ccatcgagca aaactgccag
    23401 cggacgttct tctgctgtta catcgccggt ggtaaaaatc catacacgac gtgaattttt
    23461 caaccgttca cttttccaga taatttcttt ggctgctatt tctggcgctt gcggacaatc
    23521 ccatcccggt tgcagaggcg cttgcggcat ttcgagtgca gaaacagcgt gccctcgccc
    23581 gcctttccag ctttgtggat tcagcggatc ggctattgcc tggggtaata gttttcgcca
    23641 gccttcacgt aattcgaggc gatcggggga tggtgcagaa aaaatgtcat cgcgttcggt
    23701 gggaataaag cagtagctgc cgcgccagtt ggcattgagt tgtgtcgtcc actgccagac
    23761 atcagtacct gcaattcgct gcatcgactg gggctggctg ttctgatgat gatcggtcac
    23821 accagtgatg tagatccata cgcgctttat cggcgagtat tcttcagaac cttggggatc
    23881 acgccaccag aaagtgacct caaacatttc gtcattcaga cgctgccatt ccgggccatg
    23941 tttcgactgc caccagctct cacttcctac ttttaacgcc gtcacaccat aaccccatgt
    24001 ttactgtgca atttttcatt gattgcagaa atatattgat aatattattg ataactattt
    24061 gcatttgcaa tagcgtaatg gcgcgccgtg ggaagcgcgg acatttatta accaactgca
    24121 ctgcgtgtct ttcaggatca aaggttttcg cggtagcggg atgcgtcgtg ttgaggacga
    24181 ccatgcccga cagttgcaat tcgtggcaaa aatgcaggaa taaaacaatg aacaagaaga
    24241 ttcactccct ggccttgttg gtcaatctgg ggatttatgg ggtagcgcag gcacaagagc
    24301 cgaccgatac tcctgtttca catgacgata ccattgtcgt taccgccgcc gagcagaact
    24361 tgcaggcacc tggcgtttcg accatcaccg cagatgaaat ccgcaaaaac cccgttgccc
    24421 gcgatgtgtc ggagatcatc cgtaccatgc caggcgttaa cctgaccggt aactccacca
    24481 gtggtcagcg tgggaataac cgacagattg atattcgcgg tatgggtccg gaaaacacgc
    24541 tgattttgat tgacggcaag ccggtaagca gccgtaactc ggtgcgtcag ggctggcgtg
    24601 gcgagcgcga tacccgtggt gatacctcct gggtgccacc tgaaatgatt gaacgtattg
    24661 aagttctgcg tggtccggca gctgcgcgtt atggcaacgg cgcggcgggc ggcgtggtta
    24721 acatcattac caaaaaaggc agcggtgagt ggcacggctc ctgggacgct tatttcaatg
    24781 cgccagaaca taaagaggaa ggtgccacta aacgcaccaa cttcagcctg accggtccgc
    24841 tgggcgacga attcagcttc cgcttgtatg gcaacctcga caaaacccag gctgacgcgt
    24901 gggatatcaa ccagggccat cagtccgcgc gtgccggaac gtatgccacg acgttaccag
    24961 ccgggcgcga aggggtgatc aacaaagata ttaatggcgt ggtgcgctgg gacttcgcgc
    25021 ctctgcaatc actcgaactg gaagcgggtt acagccgcca gggtaacctg tatgcgggcg
    25081 atacccagaa caccaactct gacgcttata ctcgctcgaa atatggcgat gaaaccaacc
    25141 gcctgtatcg ccagaactac tcgctgacct ggaacggtgg ctgggataac ggcgtgacca
    25201 ccagcaactg ggtgcagtac gaacacaccc gtaactcgcg tattccggaa ggtctggcgg
    25261 gcggtaccga agggaaattt aacgaaaaag cgacacagga tttcgtcgat atcgatcttg
    25321 atgacgtgat gctgcacagt gaagttaacc tgccgcttga tttcctcgtt aaccagacgc
    25381 tgacgctggg tacggagtgg aatcagcaac ggatgaaaga cttgagttcc aatacccagg
    25441 cgctgaccgg gacgaacacc ggtggcgcta ttgatggcgt gagtgccacc gaccgtagcc
    25501 cgtattcaaa agcagaaatt ttctcgctgt ttgccgaaaa caacatggag ctgactgaca
    25561 gcaccatcgt aacgccgggg ctgcgtttcg atcatcacag tattgtcggc aataactgga
    25621 gcccggcgct gaacatatcg caaggtttag gcgatgactt cacgctgaaa atgggcatcg
    25681 cccgtgctta taaagcgccg agcctgtacc agactaaccc gaactacatt ctctacagta
    25741 aaggtcaggg ttgctatgcc agcgcgggcg gctgctatct gcaaggtaac gatgacctga
    25801 aagcagaaac cagcatcaac aaagagattg gtctggagtt caaacgcgac ggctggctgg
    25861 cgggcgtcac ctggttccgt aacgattatc gcaataagat tgaagcgggc tatgtggctg
    25921 tagggcaaaa cgcagtcggc accgatctct atcagtggga taacgtgccg aaagcggtgg
    25981 ttgaaggtct ggaaggatcg ttaaacgtac cggttagcga aacggtgatg tggaccaata
    26041 acatcactta tatgctgaag agtgaaaaca aaaccacggg cgaccgtttg tcgatcatcc
    26101 cggagtatac gttgaactca acgctgagct ggcaggcacg ggaagatttg tcgatgcaaa
    26161 cgaccttcac ctggtacggc aagcagcagc cgaagaagta caactataaa ggtcagccag
    26221 cggttggacc ggaaaccaaa gaaattagtc cttacagcat tgttggcctg agcgcgactt
    26281 gggatgtgac gaagaatgtc agtctgaccg gcggcgtgga caatctgttc gacaaacgtt
    26341 tgtggcgtgc gggtaatgcc cagaccacgg gcgatttggc aggggccaac tatatcgccg
    26401 gtgccggggc gtatacctat aacgagccgg gacgtacgtg gtatatgagc gtaaacaccc
    26461 acttctgatg ctaacgtcag attgttgaca aagtgcgcgt cgttcatgcc ggatgcggcg
    26521 tgaacgcctt atccggccta caaaaatctt gccaattcaa tatattgcag gaccatgttg
    26581 gcctgataag cgtagcgcat caggcagttt tgcgtttgtc atcagtctcg aatatggtcg
    26641 atatgaaaac tacgcatacc tccctcccct ttgccggaca tacgctgcat tttgttgagt
    26701 tcgatccggc gaatttttgt gagcaggatt tactctggct gccgcactac gcacaactgc
    26761 aacacgctgg acgtaaacgt aaaacagagc atttagccgg acggatcgct gctgtttatg
    26821 ctttgcggga atatggctat aaatgtgtgc ccgcaatcgg cgagctacgc caacctgtct
    26881 ggcctgcgga ggtatacggc agtattagcc actgtgggac tacggcatta gccgtggtat
    26941 ctcgtcaacc gattggcatt gatatagaag aaattttttc tgtacaaacc gcaagagaat
    27001 tgacagacaa cattattaca ccagcggaac acgagcgact cgcagactgc ggtttagcct
    27061 tttctctggc gctgacactg gcattttccg ccaaagagag cgcatttaag gcaagtgaga
    27121 tccaaactga tgcaggtttt ctggactatc agataattag ctggaataaa cagcaggtca
    27181 tcattcatcg tgagaatgag atgtttgctg tgcactggca gataaaagaa aagatagtca
    27241 taacgctgtg ccaacacgat taattgacaa catctggtac gattcgcccg cagccatcac
    27301 tgaccacggg cgaaagtgta aagcaggtgc cttaccatcc tgacctgaca accggatatg
    27361 cggggatt
//
LOCUS       DAJOWB010000057        27201 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJOWB010000057 DAJOWB010000000
VERSION     DAJOWB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.057"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(287..2860)
                     /gene="clpB"
                     /locus_tag="OHO85_002567"
     CDS             complement(287..2860)
                     /gene="clpB"
                     /locus_tag="OHO85_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCQ2671927.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2990..3721)
                     /gene="pgeF"
                     /locus_tag="OHO85_002568"
     CDS             complement(2990..3721)
                     /gene="pgeF"
                     /locus_tag="OHO85_002568"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCQ2671928.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAADAKASTAFIQHGDKYLADIYLLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3718..4698)
                     /gene="rluD"
                     /locus_tag="OHO85_002569"
     CDS             complement(3718..4698)
                     /gene="rluD"
                     /locus_tag="OHO85_002569"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCQ2671929.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIIINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4833..5570
                     /gene="bamD"
                     /locus_tag="OHO85_002570"
     CDS             4833..5570
                     /gene="bamD"
                     /locus_tag="OHO85_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCQ2671930.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5841..6182
                     /gene="raiA"
                     /locus_tag="OHO85_002571"
     CDS             5841..6182
                     /gene="raiA"
                     /locus_tag="OHO85_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCQ2671931.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6285..6410
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6286..6333
                     /gene="pheL"
                     /locus_tag="OHO85_002572"
     CDS             6286..6333
                     /gene="pheL"
                     /locus_tag="OHO85_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCQ2671932.1"
                     /translation="MKHTPFFFAFFFIFP"
     gene            6432..7592
                     /gene="pheA"
                     /locus_tag="OHO85_002573"
     CDS             6432..7592
                     /gene="pheA"
                     /locus_tag="OHO85_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCQ2671933.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTCLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSKNVVPVDPT"
     gene            complement(7635..8756)
                     /gene="tyrA"
                     /locus_tag="OHO85_002574"
     CDS             complement(7635..8756)
                     /gene="tyrA"
                     /locus_tag="OHO85_002574"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCQ2671934.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8767..9837)
                     /gene="aroF"
                     /locus_tag="OHO85_002575"
     CDS             complement(8767..9837)
                     /gene="aroF"
                     /locus_tag="OHO85_002575"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCQ2671935.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            10047..10412
                     /locus_tag="OHO85_002576"
     CDS             10047..10412
                     /locus_tag="OHO85_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCQ2671936.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10562..11080
                     /locus_tag="OHO85_002577"
     CDS             10562..11080
                     /locus_tag="OHO85_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCQ2671937.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            11070..12296
                     /gene="dgcN"
                     /locus_tag="OHO85_002578"
     CDS             11070..12296
                     /gene="dgcN"
                     /locus_tag="OHO85_002578"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCQ2671938.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISITSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12312..12794
                     /locus_tag="OHO85_002579"
     CDS             12312..12794
                     /locus_tag="OHO85_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCQ2671939.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12870..13217)
                     /gene="rplS"
                     /locus_tag="OHO85_002580"
     CDS             complement(12870..13217)
                     /gene="rplS"
                     /locus_tag="OHO85_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCQ2671940.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13259..14026)
                     /gene="trmD"
                     /locus_tag="OHO85_002581"
     CDS             complement(13259..14026)
                     /gene="trmD"
                     /locus_tag="OHO85_002581"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCQ2671941.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(14057..14605)
                     /gene="rimM"
                     /locus_tag="OHO85_002582"
     CDS             complement(14057..14605)
                     /gene="rimM"
                     /locus_tag="OHO85_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCQ2671942.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14624..14872)
                     /gene="rpsP"
                     /locus_tag="OHO85_002583"
     CDS             complement(14624..14872)
                     /gene="rpsP"
                     /locus_tag="OHO85_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCQ2671943.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15009..16370)
                     /gene="ffh"
                     /locus_tag="OHO85_002584"
     CDS             complement(15009..16370)
                     /gene="ffh"
                     /locus_tag="OHO85_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCQ2671944.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16537..17328
                     /locus_tag="OHO85_002585"
     CDS             16537..17328
                     /locus_tag="OHO85_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCQ2671945.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17349..18635
                     /locus_tag="OHO85_002586"
     CDS             17349..18635
                     /locus_tag="OHO85_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCQ2671946.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18690..19283)
                     /gene="grpE"
                     /locus_tag="OHO85_002587"
     CDS             complement(18690..19283)
                     /gene="grpE"
                     /locus_tag="OHO85_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCQ2671947.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19406..20284
                     /gene="nadK"
                     /locus_tag="OHO85_002588"
     CDS             19406..20284
                     /gene="nadK"
                     /locus_tag="OHO85_002588"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCQ2671948.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20370..22031
                     /gene="recN"
                     /locus_tag="OHO85_002589"
     CDS             20370..22031
                     /gene="recN"
                     /locus_tag="OHO85_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCQ2671949.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22180..22521
                     /gene="bamE"
                     /locus_tag="OHO85_002590"
     CDS             22180..22521
                     /gene="bamE"
                     /locus_tag="OHO85_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCQ2671950.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22583..22873)
                     /locus_tag="OHO85_002591"
     CDS             complement(22583..22873)
                     /locus_tag="OHO85_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCQ2671951.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22863..23300)
                     /gene="ratA"
                     /locus_tag="OHO85_002592"
     CDS             complement(22863..23300)
                     /gene="ratA"
                     /locus_tag="OHO85_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCQ2671952.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            23471..23953
                     /gene="smpB"
                     /locus_tag="OHO85_002593"
     CDS             23471..23953
                     /gene="smpB"
                     /locus_tag="OHO85_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCQ2671953.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24168..24530
                     /gene="ssrA"
                     /locus_tag="OHO85_002594"
     tmRNA           24168..24530
                     /gene="ssrA"
                     /locus_tag="OHO85_002594"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24715..25914
                     /locus_tag="OHO85_002595"
     CDS             24715..25914
                     /locus_tag="OHO85_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2671954.1"
                     /translation="MARTTRPLTNTEVLRAKALEKDLTLHDGDGLFLIVKTSGKKLWR
                     FRYQRPATKQRTMMGLGAFPALSLADARGLRADYLALLANGIDPQIQAEVAEEQQQIA
                     LDSIFSTVAANWFQLKSKSVTHDYAKDIWRSLEKDVFPTIGEIPVQQIKARTLVEALE
                     PIKARGALETVRRLVQRVNEIMIYAVNTGLIDANPASGIGMAFEKPKKQNMPTLRPEE
                     LPMLMRSLVMSNLSVPTRCLIEWQLLTLVRPSEASGARWAELDLDAKLWTIPAERMKA
                     KREHIVPLSPQALEILDVMKLISAHREHVFPSRNDPKQPMNSQTANAALKRIGFGGRL
                     VAHGLRSIASTALNEAGFNADVIEAALAHSDKNEVRRAYNRSTYLEQRIGLMNWWGQK
                     VYERNQY"
     gene            26088..26402
                     /locus_tag="OHO85_002596"
     CDS             26088..26402
                     /locus_tag="OHO85_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2671955.1"
                     /translation="MINNTTDPELIAAITYFKEHPPKKQIIEDDSLVWDDSLPQEKDL
                     AKFLFLLIRRVRNNLFHGGKFNGKWFEPERSEALLQHGLVILRHCGTRHSEVSKAYSG
                     IA"
     gene            complement(26495..>27201)
                     /locus_tag="OHO85_002597"
     CDS             complement(26495..>27201)
                     /locus_tag="OHO85_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2671956.1"
                     /translation="SLTFPHELVVRHPGISPLLMQNLWDRFSRDTNKPVERLLLSEPG
                     SNDALDSYVSAFSRISDTLSKKLGYNSKGAYVQALLVSKWMKGYPLARLISDRIRYNK
                     KKNIVFKEATLIRGVMKDVEEIARYQAPRLLGCYNDLLKSFYLSIDRTDLAKDVEDIG
                     VYLELGVSLKTQISLIGLGFSRTAAVMISELIPNDSLDEEECINWIKDNKVDMEELPQ
                     LVQNEIHNIFRNFWVQ"
BASE COUNT         7002 a         7072 c         6334 g         6793 t
ORIGIN      
        1 ttttcccagc ggcgcggggt gtgcataata cgctttcccg ctacagagtc aagcattttt
       61 tttgcttttc tctgttgaga ttctcaggag aaccccgctg ccccggcggc gtgtttgccg
      121 ttgttccgtg tcagtggtgg cgcattatag ggagttattc cggcctgaca agcgaaaaag
      181 ataaaaactt tatcgttcgc tcatttttca ggcaaaacat cttaaatata gtcttttccg
      241 tctaacttat agacaaaaac gagccccgaa gggctcgttt tatcatttac tggacggcga
      301 caatccggtc ttcattaact tccaggcgaa tcactttacc cggaaccaat tcaccagaca
      361 gtatttgctg tgccagcggg ttttcgatct gctgctgaat tgcacgtttc agaggacgtg
      421 caccatagac cggatcgtaa ccgttctcgc tcagcagttt cagcgcctcg tcagaaatgt
      481 ggatttcata accacgttct tccagacgtt tatacagacg tttcaactga atctgcgcaa
      541 tcgacgcaat gtgctgttca cccagcggat ggaagaccac cacttcatcg atacggttaa
      601 tgaattccgg acggaagtta tggctaacca caccgagcac cagctctttc atgtgcgcat
      661 aatccagttc accgaagcgt tcctgaatca gatcggaacc gaggttagag gtcataatga
      721 cgaccgtatt acggaagtcg accgttctcc cctgcccgtc agtcagacgc ccatcatcca
      781 gtacctgcaa cagaatgttg aagacatccg gatgcgcttt ttccacttca tccagcagga
      841 tgacggaata cggacgacga cgcaccgctt cggtcaggta gccaccttct tcataaccga
      901 catatcccgg aggcgcacca accaaacgag acaccgagtg tttctccata aactcggaca
      961 tatctatacg gaccatcgcc tcgtcgctat caaacataaa gttcgccagc gccttacaaa
     1021 gctctgtttt ccccacaccc gttgggccga ggaacaggaa tgaaccaatc gggcgatttg
     1081 gatccgccaa ccccgcacgg ctacggcgaa tagcgttaga taccgcatca accgcttcgt
     1141 tctgaccaat gacgcgatgg tgcagttctt gctccatacg cagcagtttt tcgcgctcgc
     1201 tttccatcat gcgagaaacc ggaatccccg tccaacgcgc cagcacttca gcaatttcgg
     1261 cgtcggtcac tttattacgc aacagacgca tagttttgcc ttcgagctgc gttgcggctt
     1321 ccagttgctt ttccagttcc gggattttgc cgtattgcag ttcagacatc cgcgccaggt
     1381 cccccacacg gcgagcctgt tcaatagcga ttttcgcctg ttccagttcc gctttaatgg
     1441 tctgcgtacc agaaagcgat gccttctctg ctttccactc ttcttctaac tcggagtact
     1501 gacgttcttt gtcgctcagt tcttcgttga gcatatccag gcgtttttta ctggcttcat
     1561 cagactcttt cattaacgcc tgttgttcca gtttgagctg gatgatacga cgatcgagtc
     1621 ggtcgagttc ttctggtttt gagtcaatct gcatacgaat gctggatgct gcttcatcga
     1681 tcaggtcgat ggctttatcc ggcagctgac ggtcagcaat gtagcgatga gacaacgtcg
     1741 ccgctgcaac aattgccggg tcagtaattt gcacatggtg gtgcaattcg taacgttctt
     1801 tcaggccacg cagaatcgca atggtatctt caacagaagg ctcggcaaca aacactttct
     1861 ggaaacgacg ttccagcgca gcatcttttt cgatgtactg gcgatattcg tcaagcgtcg
     1921 tggcacctac gcagtgcaat tcaccacgcg ccagcgccgg tttcagcatg tttccggcgt
     1981 ccattgcgcc atcggcttta cccgcgccga ccatggtatg taattcgtcg ataaacagga
     2041 tgacgttgcc ttcctgtttg gcaagatcgt taagcacgcc ttttaaacgt tcttcaaact
     2101 caccgcgata tttcgcccca gccaccagcg cgcccatatc cagcgccagt acgcggcggc
     2161 ctttcaatcc ttccggcact tcgccgttga taatacgctg cgccagacct tcaacgatgg
     2221 cagttttacc gacgccgggt tcaccaatca gtaccgggtt atttttagta cgacgttgca
     2281 gcacctgaat ggtacggcga atttcttcat cacgaccaat caccggatcg agtttgccct
     2341 gttcggctcg ttcggtaagg tcgatggtat attttttcaa agcctgacgt tggtcttcag
     2401 caccttgatc gttcacgctt tcacctccac gcatttgttc aatcgcttga gtaatgttgg
     2461 cggtggtcgc ccctgctgct ttcaggatgt cggccagcgt gccgcgagac tcaagtgccg
     2521 ccagaacgaa cagttctgac gagataaagt tatcaccacg tttttgcgcc agcttgtcgc
     2581 aaagattaag aacgcgcacc agatcctgtg atggctggac atcaccacca gtaccttcaa
     2641 cctgcggtaa acgatttaat gcctgattga tatctgtgcg caactggcca gcatttatgc
     2701 cagctgatgt taacaaagga ctaaccgaac ccccttcctg attcagcagg gcgctcatta
     2761 aatgaagtgg ttcgatgaat tggttgtcgt gcccgagtgc aagtgattgg gcatcggcaa
     2821 gagcaagctg gaatttatta gtaagacgat ccagacgcat aactcctccc ataacggatc
     2881 aaagttgcta ctggagatta aatgaggtca tccctcaatt attcaaggtt attgaccaga
     2941 ttaatgtgaa aagaaaatca cgcgtaccgg atcgtcttga ttctttaggt tatatcagcc
     3001 aaatgaaact tgccatacgc ccggtggtct tgtcgcgacg ataagagaag aaagtctcat
     3061 tttccgtata tgtacaacgg tcgccgccga aaatttgttc aacaccaacg ctcgccagac
     3121 gctgccgggc aagcagataa atatccgcca gatacttatc accatgctga atgaaagctg
     3181 tactcgcttt ggcgtctgct gccataaacg cctcgcgaac ctccgccccc acttcgaacg
     3241 cgcgtggacc aattgccggc cctaaccagg cgagaatatt ttccggatta tcagcaaaac
     3301 aggaaactgt ctcttccagc acgcctgcgc acagtccacg ccagccagca tgagcggcgg
     3361 cgacttccgt tcccgctcga ttgcaaaaca gcacagggag gcagtcggca gtcatcactg
     3421 cgcaaaccgt gccaggcgta ttgctataag aggcatctgc tcgttttgag gcataaggtt
     3481 cgccagtaag cttaagcaca tctttgccgt gtacctgctc aagccagacc ggtttagaag
     3541 gcaaattgcc cgcagcaaaa agtcgcttgc gattctcctc aacgtgatcc gggttatcgc
     3601 cacaatgggc accgaggttg agcgagtcat acgggggcaa gctcacgccg ccgatacgag
     3661 tagagctaca agccgcaaca ccctttggca gcggccactg cgggacaatc agcttactca
     3721 taaccagtcc acttcatcct tatgttcttc gaaatcggcg cgcatcacct caatcagctc
     3781 caccatatct tgtggaatag gcgcatgcca ttccatttcg atgccggaga tcgggtgata
     3841 aagacgcagc atggttgcat gtagcgcctg gcggtcaaac ttacgcagcg tggagataaa
     3901 tgcttccgaa gcaccttttg gcggacgcgg acggccacca taaaccggat cgcccaccag
     3961 cggatgagtg atatgggcca tatgcacgcg gatctggtgc gtacgtccag tttccagacg
     4021 caaccgcaga cgcgtgtgca cacggaagtg ttccatgatg cgatagtgag tcaccgctgg
     4081 tttgcccatc ggatgcaccg ccatatgggt acgtttggtc gggtggcgac tgattggctc
     4141 gtccaccgtg ccacctgcgg tcatatgacc aatcgccacc gcttcatact cacgagtaat
     4201 ttcacgccgt tgcaaagatt cgactaaacg cgtctgagcc ggaacggttt ttgccacaac
     4261 catcaggcca gtggtgtctt tatccagacg atggacgatg cccgcacgcg gtacatcggc
     4321 aatgggtgga tagtaatgaa gcaacgcatt cagtaccgtg ccatccgggt tacccgcgcc
     4381 aggatgtacc accaggtcgc gcggtttatt aatgataata atgtcttcat cttcatagac
     4441 gatatccagc gggatatcct gcggttcaaa acgcgcttct tcttcaatct cagcgttgat
     4501 ggcaacctgc tcgccaccca atactttttc tttcggctta tcacaaactt tgccgttaac
     4561 cagcactcgc tggtcgagga tccattcttt tattcgcgaa cgtgaataat ccgggaacat
     4621 ttcggccaaa gcctgatcta agcgttgacc gagttggttt tcggacaccg ttgcagtgag
     4681 ctgtactcgt tgtgccatat atactgcttc ttcgtttatc gttggggttt tacggctttg
     4741 ccgtttaata tagtgtgcta ttgtagctgg tcttaaccgg gagcaggaac agagaatctc
     4801 ccgtattaca ttttgaggaa agtcaaaacg tcatgacgcg catgaaatat ctggtggcag
     4861 ccgccacact aagcctgttt ttggcgggtt gctcggggtc aaaggaagaa gtacctgata
     4921 atccgccaaa tgaaatttac gcgactgcac aacaaaagct gcaggacggt aactggagac
     4981 aggcaataac gcaactggaa gcgttagata atcgctatcc gtttggtccg tattcgcagc
     5041 aggtgcagct ggatctcatc tacgcctact ataaaaacgc cgatttgccg ttagcacagg
     5101 ctgccatcga tcgttttatt cgccttaacc cgacccatcc gaatatcgat tatgtcatgt
     5161 acatgcgtgg cctgaccaat atggcgctgg atgacagtgc gctgcaaggg ttctttggcg
     5221 tcgatcgtag cgatcgcgat cctcaacatg cacgagctgc gtttagtgac ttttccaaac
     5281 tggtgcgcgg ctatccgaac agtcagtaca ccaccgatgc caccaaacgt ctggtattcc
     5341 tgaaagatcg tctggcgaaa tatgaatact ccgtggccga gtactataca gaacgtggcg
     5401 catgggttgc cgtcgttaac cgcgtagaag gcatgttgcg cgactacccg gatacccagg
     5461 ctacgcgtga tgcgctgccg ctgatggaaa atgcataccg tcagatgcag atgaatgcgc
     5521 aagctgaaaa agtagcgaaa atcatcgccg caaacagcag caatacataa cagaaacctg
     5581 aaacacaaaa cggcagccct tgagctgccg tttttttatt ctgtcagttg tgaaactgaa
     5641 ccgatttagt cgctatcgat ctcatcaaat atggctcgct ttgagatatt cctcaagtaa
     5701 aaaaacacct cttcctgcga tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat
     5761 tatgagattt tcatcacaca ttttgacatc aggaacggta tgctgaattc accaagacgg
     5821 gaagacaaga ggtaaaattt atgacaatga acattaccag caaacaaatg gaaattactc
     5881 cggccatccg ccaacatgtc gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc
     5941 tgattaatcc acatatcatt ctgtccaaag agccacaagg gtttgttgct gacgccacaa
     6001 tcaatacacc taacggcgtt ctggttgcca gtggtaaaca tgaagatatg tacaccgcaa
     6061 ttaacgaatt gatcaacaag ctggaacggc agctcaataa actgcagcac aaaggcgaag
     6121 cacgtcgtgc cgcaacatcg gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt
     6181 agtcctttat attgagtgta tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt
     6241 gaaaacagta cgggtactgt actaaagtca cttaaggaaa caaacatgaa acacacaccg
     6301 tttttcttcg cattcttttt tatcttcccc tgaaagggag gcgtttcgtc gtgtgaaaca
     6361 gaatgcgaag acgaacaata aggcctccca aatcgggggg ccttttttat tgataacaaa
     6421 aaggcaacac tatgacatcg gaaaacccgt tactggcgct gcgagagaaa atcagcgcgc
     6481 tggatgaaaa attattagca ttactcgcag agcggcgcga actggccgtc gaggtgggaa
     6541 aagccaaact gctctcgcat cgcccggtac gtgatattga tcgtgaacgc gatttactgg
     6601 aaagattaat tacgctcggt aaagcgcacc atctggacgc ccattacatt actcgcctgt
     6661 tccagctcat cattgaagat tccgtattaa ctcagcaggc tttgctccaa caacatctca
     6721 ataaaattaa tccgcactca gcacgcatcg cttttctcgg ccccaaaggc tcctattcac
     6781 atcttgccgc tcgtcagtac gctgcccgtc actttgagca attcattgaa agtggctgcg
     6841 ccaaatttgc cgatattttt aatcaggtgg aaaccggcca ggccgactat gccgtcgtac
     6901 cgattgaaaa taccagctcc ggtgccataa acgacgttta cgatctgctg caacatacct
     6961 gcttgtcgat tgtgggcgaa atgaccctta ccatcgacca ctgcctgctg gtttccggca
     7021 ccacagattt atctaccatc aacacggtct acagtcatcc acagccgttc cagcaatgca
     7081 gcaaattcct taatcgttat ccgcactgga agattgaata taccgaaagt acgtctgcgg
     7141 caatggaaaa ggttgcacag gcaaaatcac cgcatgttgc tgcgttggga agcgaagctg
     7201 gcggcacttt gtacggtttg caggtactgg agcgtattga agcgaatcag cgacaaaact
     7261 tcacccgatt tgtggtgttg gcgcgtaaag ccattaacgt gtctgatcag gttccggcga
     7321 aaacgacgtt gttaatggcg accgggcaac aagccggtgc gctggttgaa gcgttgctgg
     7381 tgctgcgcaa ccacaatctg attatgaccc gtctggaatc acgcccgatt cacggtaatc
     7441 catgggaaga gatgttttat ctggatattc aggccaatct tgaatcagcg gaaatgcaaa
     7501 aagcattgaa agagttaggg gaaattaccc gttcaatgaa ggtattgggc tgttacccaa
     7561 gtaagaacgt agtgcctgtt gatccaacct gatgaaaagg tgccggatga tgtgaatcat
     7621 ccggcactgg attattactg gcggttgtca ttcgcctgac gcaataacac gcggctttca
     7681 ctctgaaaac gctgtgcgta atcgccgaac cagtgctcca ccttgcggaa actgtcaata
     7741 aacgcctgct tatcgccctg ctccagcaac tcaatcgcct cgccgaaacg cttatagtaa
     7801 cgtttgatta acgccagatt acgctctgac gacataataa tgtcggcata aagctgcgga
     7861 tcctgagcaa acagtcgccc gaccatcgcc agctcaaggc ggtaaatcgg cgaagagagc
     7921 gccagaagtt gctcaagctg aacattttct tctgccagat gcagcccata agcaaaagta
     7981 gcaaagtggc gcagagcctg aataaacgcc atattctgat cgtgctcgac agcgctaata
     8041 cgatgcagcc gagcgcccca gacctgaatt tgctccagaa accattggta tgcttccggc
     8101 ttacgtccat cacaccagac cacaacttgc tttgccaggc taccgctgtc cgggccgaac
     8161 atcgggtgta accccagtac cgggccatcg tgcgccgcca gcatggcctg taatggtcca
     8221 tttttcactg atgccagatc aaccagaata caatctttcg gtaaaggcgg taatttgccg
     8281 ataacttgct cagtaacgtg gattggcaca ctaacaatca ccattccggc atcggcaaca
     8341 atatcagccg ctcgatccca gtcatgttgc tccagaatcc gcacctgata acccgatagt
     8401 gtcagcatct tctcgaacag gcgtcccatc tgaccgccac cgccgacgat aaccaccgga
     8461 cgcagtgacg gacaaagtgt tttaaatcct ttgtcgtttt cactggagta agattcacgc
     8521 atcacccgac gcaaaacatc ctcaatcaga tctggcggta cacccagagc ttccgcctct
     8581 gcgcgccgcg aggccaacat agatgcctcg cgctccggaa cataaatagg cagtccaaag
     8641 cggcttttca cctcgcccac ttcagcaacc agttccagac gcttcgctaa taaattcagc
     8701 agcgctttat cgacttcatc aatttgatcg cgtaatgcgg tcaattcagc aaccataata
     8761 aacctcttaa gccacgcgag ccgtcagctg cccgttcaga tcctgatgaa tttcacgcag
     8821 caaggcatcg gtcatttccc agctaatgca ggcatcggtt acggatacac cgtatttcat
     8881 ttcactgcgc ggttgctcgg aagactgatt gccctcgtgg atattacttt cgatcatcag
     8941 accaataatt gagcgattgc catctttgat ttgagcaacc acggattctg ccaccgcagg
     9001 ctgacggcga taatctttat tggaattacc gtggctgcaa tctaccatca gagacgggcg
     9061 cagtcccgcc tgttccatct ctttttcaca ttgcgcaaca tccgcagggc tatagttcgg
     9121 cgctttacca ccgcgcagga tcacatggcc gtccggattc ccctgagttt gtagcaacgc
     9181 aacctgccct gcctggttaa tgccaacaaa acggtgcggc tgggcggcgg cgcgcatagc
     9241 gttaattgct gttgccagac tgccgtcggt gccgttttta aaaccaaccg gcatggaaag
     9301 cccggaggcc atttcacggt gagtttgcga ttccgttgta cgagcaccaa ttgctgacca
     9361 gctaaacaga tcgcccaggt attgcgggct attcggatct aacgcttccg tcgccagtgg
     9421 cagtcccata ttcaccagct caagcagcaa tttacgcgcg atctgcagcc cggcttctac
     9481 atcaaaagag ccatccatat ggggatcgtt aattaaccct ttccagccga cagtggtacg
     9541 gggtttttca aaatagacgc gcattaccag atagaggcta tcgctgacct ctgcggcaag
     9601 ggctttaaat cgacgagcat attccagagc agtttccgga tcatgaatgg aacaaggacc
     9661 acatactacc agcagacgag gatcgcgccc ggcgataata tctgaaatgg ttttacgcga
     9721 gtcagcaatc tgggcttctt gttgcaggct caatggaaaa gcggccttca gttgttccgg
     9781 agtcattaaa acctgttcgt cggtaatatg tacgttattc agcgcgtctt tttgcatgat
     9841 ggcgatcctg tttatgctcg tttgcgatag ttgatcctca gcgaggatga cgtaacgata
     9901 acacataaag taaagttttc aatccatatt tcgtacattt ttatttacac aggcaattta
     9961 gtcgcgattt caacccttac ttctgtatag ataaatttac actccctttg aaaacaatcc
    10021 gctatgcttt gaaaaaggag aaagaaatga tgaaaaagtt tatcgccccc ttgttggctt
    10081 tactggttag cggatgtcag attgatcctt atactcacgc gccaaccttg accagcaccg
    10141 actggtatga tgttggtatg gaagatgcga tatcgggcag cgccataaaa gatgacgatg
    10201 catttagcga ttcacaggcg gatcgcggtc tataccttaa aggatatgcc gaaggacaaa
    10261 agaaaacttg ccagaccgat tttacttatg cccgaggact ttccggtaaa agctttcctg
    10321 ccagctgcaa caatgttgaa aatgccagcc aactgcatga agtatggcaa aaaggggctg
    10381 atgaaaatgc cagcacaata cgattaaatt aattaaaacc ccacaaataa attaaacata
    10441 agattttact cattgtgttt tatttctcac attgatgacg atcgcatgaa aaaatgatat
    10501 tttaatgagc attctgaaaa aatatcgatg catttcgagc gaagatggtg gggatccctg
    10561 aatgcgtttt tctcaccgac tgttcttact tctgattctc ctgctgacgg gagcaccaat
    10621 actcgctcag gagccttctg acgttgcgaa aaacgtgcgt atgatggttt ccggtattgt
    10681 cagttacacc cgctggcctg cgctatcagg gccaccaaag ttatgtatct tttcatcttc
    10741 acgttttagt accgccctac aagagaatgc cgcgacatct ttgccttatc tgcctgtcat
    10801 cattcacact caacaagagg cgatgatttc aggctgcaat ggtttttatt ttggcaacga
    10861 gtcaccgaca ttccagatgg aattaaccga acaatatccg tcaaaggcgt tattattaat
    10921 tgccgaacaa aataccgagt gcattattgg tagcgccttt tgtctgatca tccacaataa
    10981 tgacgtcaga tttgccgtaa acctggatgc cttatcgcgt agcggtgtaa aggtcaaccc
    11041 ggatgtctta atgctcgcac ggaagaaaaa tgatggataa cgataattct cttaataagc
    11101 gccccacgtt taaaagagca ttacgcaaca tcagtatcac cagcatattt atcactatga
    11161 tgctgatctg gttgctgctt tccgtgacct cggtgctgac cctgaaacag tacgcgcaaa
    11221 aaaacctggc actgacagca gcaacaatga cttacagtct ggaagcagct gtcgtttttg
    11281 ccgatggccc tgcagcaact gaaacactgg cagcgctggg ccagcaaggg caattttcaa
    11341 ctgcagaagt acgtgataag cagcaaaata ttctggcgtc ctggcattac acccgtaagg
    11401 atccaggcga tactttcagc aatttcataa gccactggct cttccccgcc cccatcattc
    11461 agccgattcg tcacaatggt gaaaccattg gcgaagtacg cttaaccgct cgcgacagtt
    11521 caatcagcca ttttatctgg ttttcgctcg ccgtactgac cggttgtatt ctgctggcat
    11581 caggcatcgc aattaccctc acccgccatt tgcacaatgg cctggtggaa gcactgaaaa
    11641 atatcaccga tgtcgtacat gatgtgcgtt ccaaccgcaa tttttcccga cgagtttcgg
    11701 aagaacgtat cgctgagttt caccgcttcg ctctcgactt caacagtctg ctggatgaaa
    11761 tggaagagtg gcagcttcgt ttacaggcta aaaatgcgca gcttctacgt accgcgctac
    11821 atgacccatt aaccgggctg gctaaccgcg cagcgtttcg tagcggcatc aacacgttga
    11881 tgaacaattc cgatgcccga aaaacgtcgg cgttactatt tcttgatggc gataatttca
    11941 aatacatcaa tgatacctgg ggtcatgcga cgggcgatag agtcttgatt gaaatcgcaa
    12001 aacggttagc tgaagttggc gggctgcgac ataaagcata ccgcctgggc ggcgatgaat
    12061 tcgctatggt gctctatgat gtacagtcag aatctgaagt gcagcagata tgctcagcac
    12121 tgacacaaat ctttaatctc ccgtttgatc ttcataatgg tcatcagacc accatgacat
    12181 taagcattgg ttacgcgatg accattgagc acgcctctgc ggaaaaatta caagagcttg
    12241 ccgatcacaa tatgtatcag gccaaacacc agcgtgccga aaagctggtg agataacaag
    12301 gatatatcga tatgataaag cacctggtag cacccctgat tttcacctca ctaatactga
    12361 ctggctgcca gtcccctcag ggaaagttta ctcctgagca agtcgccgct atgcaatctt
    12421 atggatttac tgaatccgcc ggcgactggt cgctgggctt atcagatgcc attctgttcg
    12481 caaaaaatga ctacaaattg ctcccggaaa gccagcaaca gatccaaacc atggcagcca
    12541 aattggcctc gacagggcta acacatgccc gtatggatgg acacaccgat aactatggtg
    12601 aagacagtta caacgaaggc ttatcattga aacgggcgaa tgtcgtggcc gatgcatggg
    12661 ctatgggtgg acaaattcca cgcagcaatc tcaccacaca gggtttagga aaaaaatatc
    12721 ccatagccag taacaagacc gcccagggcc gcgccgagaa ccgccgcgtc gcagtggtga
    12781 ttactacccc ttaaaacacc agcaaacaga taaaaaaggg ccagccaatt ggccagccct
    12841 tcttaacagg atgtcgctta agcgaaatct tagttaagac gctctttgat acgagcagcc
    12901 ttaccagtac gctcacgcag gtagtacagt ttagctttac gaacagcacc acgacgtttg
    12961 acagaaatgc tgtcaactac cggagagtga gtctggaaga cacgctcaac gccttcgccg
    13021 ttggaaattt tacgaacagt gaatgcagag tgcagaccgc ggttacgaat agcgataacc
    13081 acgccctcga atgcctgcag acgttttttg gaaccttcaa caacccatac tttcacttcc
    13141 acggtatcac ccggacggaa ggaaggtacg tcctgcttca tctgctcttg ttcaagttgc
    13201 ttaataatgt tgctcataat ttaatctctt atcctgggta aactgatatc tcgggggctt
    13261 acgccatccc atcatgttta tgttgctgtt gtgcgtgttc cgttttgaac tccgccagca
    13321 accttgcttg ctcttcagtc agagccaggt tttccagaag ttcaggtctt ctaagccagg
    13381 tacggcccag cgactgtttc aaacgccagc gacgtatctc ggcatggttg cccgacagta
    13441 acactggcgg aacttccatc ccttctaaca cctcaggccg cgtatagtgc gggcaatcca
    13501 gcaatccttc agcaaaggaa tcttccgttg ccgaggcttc atgtcccagt actcccggaa
    13561 taaaccggga aacggagtca atcagcgtca ttgctggtaa ctcaccacca ctgagaacgt
    13621 aatcgccgat tgaccattct tcgtcaattt cggtttggat cacgcgctca tctatacctt
    13681 cgtagcgacc gcacaccaga atcaattttt gattcgttgc cagttcgctg acgcccgctt
    13741 gatcaagctt gcgtccctgt ggtgacagat aaatcacctt tgcgccttca cccgccgcgg
    13801 cttttgctgc atgaatggcg tcccgcaagg gttgcaccat cattaacatc cccggtccgc
    13861 cgccgtaagg acgatcgtcc acggtacggt gccggtcatg cgtgaagtcg cgaggactcc
    13921 agctctggat gctcagcagg ccatttttaa ctgcccggcc agttaccccg taatcggtaa
    13981 ttgcgcggaa catttcagga aacaggctaa ttatgccaat ccacatagcg ccgtctttta
    14041 ccgtttatcc ggtggtttaa aaaccaggat cccaatctac ttcgattgaa cgagtagtga
    14101 gatcgacttt cttgataacc tgcccatcga ggaacggtac gagacgttcc ttgataccaa
    14161 acgcatcttt caggtttgcc ttaatgacga gaacgtcatt agatccggtt tccatcatat
    14221 cgacgacttt accgagatcg tagccttcag tggttactac ctggcagccc atcaggtctt
    14281 tccagtagta gtccccctct tcaagctgag gcagctgcga tgaatccacg acaatttcac
    14341 aattcgtcag caggttcgcc gcatcacgat cgtcaacgcc tttcagcttg atgatcatgt
    14401 cctgattgtg gtgcttccag ctttccagct ggacttgctg ccactgaccc gccttctgga
    14461 taaaccaggg ctgatagtca aaaatgcttt cggcgtcttc ggtggaagaa aacactctga
    14521 gccacccacg aataccgtaa gacgaaccca tttttcccaa aacgatggga tcaacaggtg
    14581 cttgcgcggt gagttgtttg ctcatcatga ccaccgtgac agattaagct gctttgttta
    14641 cttctttgat cagcgcagca acgcgatcag aaatagttgc gccctggcca acccagtgag
    14701 cgatgcgatc cagatccagg cgagtgcctt cttctttttc gctagcgatt gggttgaaga
    14761 aaccaacgcg ctcgatgaag cgaccgttgc gtgcattacg gctgtcagcg acaacaacct
    14821 ggtagaacgg acgcttttta gcgccgtgac gtgctaaacg aatagttacc ataacatcct
    14881 cttgtgtgaa taaaacaacc ggaccccatc gaggaacgga gtccggtgtc atattaaaag
    14941 cccgaaaatt ttactgattt ctggagaaaa tgcaatcagc agttaataac tctgtcgcat
    15001 aaggtcattt agcgaccagg aaatcccggc ggcatcatcc ccttcatgct tctcatcatc
    15061 ttcgccattc cgcccttctt cattttcttc atcatgcgct gcatgtcgtc gaactgcttc
    15121 agaaggcggt taacgtcctg cacctgcata ccgcaaccgg cagcgatacg acgcttacgc
    15181 gaacctttga tgatttctgg cttagcgcgc tctttcatcg tcatcgagtt gatgatggct
    15241 tccatacgca ccagcacttt atcgtccatc tgcgacttaa cattatccgg gatctgcccc
    15301 atgcccggca gcttgcccat caggctagcc atgccgccca tatttttcat ctggcgcagt
    15361 tgctcaagaa aatcgttgag atcgaagccg tcgccttttt tcagcttgct ggctaatttc
    15421 tctgcctgcg cgcggtcaac tttgctttcg atatcttcga tcagcgacag tacgtcgccc
    15481 atgccgagaa tacgcgacgc gatgcggtcc ggatggaacg gctccagcgc ctcagttttc
    15541 tcgccaacac cgagaaactt gatcggtttg ccagtgatgt gacgaataga gagcgccgca
    15601 ccgccgcggg catcgccgtc cactttggtc aacactacgc cggtaagcgg taacgcttca
    15661 ttgaatgctt ttgccgtatt ggccgcatcc tgaccggtca tggcgtcaac cacaaacagg
    15721 gtttcaaccg ggttaatcga cgcatggact tgtttgatct cgtccatcat cgcttcgtca
    15781 acgtgcagac gaccagcggt atccaccagc agcacgtcgt agaatttcag tttggcttct
    15841 ttcagcgccg cgttaacgat atctaccggc ttctgaccaa catcagaagg gaagaaatca
    15901 acgcccacct gctctgccag cgtctcaagc tgtttgattg ccgccgggcg ataaacgtcg
    15961 gcagaaacca ccagcacttt cttcttgtgc ttctcgcgca ggaacttacc gagtttacca
    16021 acgctggttg ttttaccggc accttgcagg cccgccatca gcacgaccgc aggcggttgc
    16081 gccgccaggt tcagggtctg gttctcttcg cccatcgccg caaccagttc gttacggacg
    16141 attttgacga actcctgccc cggcgtcagg ctcttattaa cttcatgacc aaccgctttc
    16201 tcttttacgc gattgataaa ctcacgcact accggcagag cgacgtccgc ctccagcagc
    16261 gccatgcgca cttcgcgcag cgtatctttt acgttgtctt cagtgaggcg tccacggcca
    16321 ctgatattgc gcagcgtgcg cgacaaacga tcggttaaat tatcaaacat tgtctctcgc
    16381 ctggggtgga aacggttggc cgcaatcgcg acacatcatc agtattttgc cgcagtataa
    16441 catgaaggcg tctttgttgt tatgcaacgg ttggagcagc gttcacctga cgctatactg
    16501 cttctctttc ttattgctca aactgtcgac attactatgc ccgtttttgc tctgctcgcg
    16561 cttgtcgcct actccgtcag tcttgcgctg attgttcccg gtctgctgca aaaaaacggc
    16621 ggctggcggc gcatggctat tatttctgcg gtcattgcgc tggtctgcca cgcaatcgct
    16681 ctggaagccc gcatcctgcc cgacggtgat agcggacaaa acctcagcct gctgaacgtt
    16741 ggttcattgg tcagtttgat gatctgtacg gtaatgacca ttgtggcttc tcgcaatcgt
    16801 ggctggttgc tgctacccat tgtctatgcc tttgcgctta tcaacctggc gctggcgacc
    16861 ttcatgccca atgaatacat cacccatctg gaagcaacgc ctgggatgct ggtgcacatt
    16921 ggcttatcgc tcttttccta tgccacgcta attatcgccg ccctgtacgc gctgcaactg
    16981 gcgtggattg attaccaact gaagaacaag aagctggcat ttaaccagga aatgccgcca
    17041 ttgatgagta tcgagcgtaa aatgttccac atcacgcaga ttggcgtggt gctgctaacg
    17101 ctcacgcttt gcactggcct gttctacatg cacaacctat ttagcatgga aaatatcgac
    17161 aaggctgtgc tctctatcgt ggcgtggttt gtctatattg tgctgctgtg gggacattac
    17221 catgaaggat ggcgtggacg ccgcgtcgtc tggtttaacg ttgcgggcgc ggtaattctg
    17281 acactggcct acttcggcag ccgaattgtc cagcagttaa tcagctaaac ccagaaaagg
    17341 agtttcccct ggaacacatt tctactacta cgttgatcat tattctgatc atcatggtgg
    17401 tcatttcagc ctatttttcc gggtccgaaa ccggaatgat gaccctcaac cgctatcgtc
    17461 tgcgacatat ggcgaaacag ggtaatcgct cggccaaacg cgtcgaaaaa ttgctgcgta
    17521 agccagaccg cctgataagc ctggtgttaa tcggcaataa cctggtcaat attcttgcct
    17581 ccgcgctcgg cactattgtt gggatgcgtt tgtacggcga tgcgggcgtg gcaattgcga
    17641 ctggtgtgct gacttttgtc gtactggtat ttgctgaggt attgccgaaa accattgccg
    17701 cgctgtaccc ggaaaaagtc gcttatccga gtagttttct gctggctccg ctgcaaattt
    17761 tgatgatgcc gctggtctgg ttgctgaatg ctatcacccg tatgctgatg cgcatgatgg
    17821 gtatcaaaac cgatatcgtg gttagcggct ctttgagcaa agaagagttg cgcactatcg
    17881 tgcacgaatc acgctcacaa atttcccgtc gcaatcagga tatgctgctg tcggtgctcg
    17941 atctggaaaa aatgaccgtt gatgacatca tggtgccgcg cagtgaaatt atcggtattg
    18001 atatcaacga tgactggaaa tcgattctgc gccaactctc ccactcacct cacgggcgca
    18061 tcgtgctcta ccgtgattcg ctggacgacg ccatcagtat gctgcgagta cgtgaagcct
    18121 ggcggttgat gtcggagaaa aaagagttca ccaaagaaac catgctgcgc gccgcggacg
    18181 agatctattt tgtgccggaa ggtacgccgc tcagcacgca gttggtaaag tttcagcgca
    18241 acaaaaagaa agtcggcctg gtcgtcaacg agtatggaga cattcagggg ctggtgacgg
    18301 ttgaagatat tctggaagag attgtcggcg atttcactac gtcgatgtcg ccaacacttg
    18361 ccgaagaggt cacgccgcaa aacgacggtt cggtgattat cgatggcacc gccaacgtgc
    18421 gggaaatcaa caaagccttt aactggcatc taccggaaga tgatgcccgc acggttaatg
    18481 gcgtcattct tgaggcactg gaagagatcc ctgtcgcagg cacccgcgtg cgtattggcg
    18541 agtacgatat agatattctc gacgtacagg acaatatgat taagcaggta aaagtttttc
    18601 ctgtgaaacc gctgcgcgag agtgtggcgg agtaacgaaa acggccctgc attcgcatgc
    18661 agggccgtga attattacga aaacagaaat tacgcttttg ctttcgctac agttaccatc
    18721 gccgcacgaa tcgtacgacc attcagcgta taacccttct gcataatgcc cagtacgtta
    18781 cctggcgcaa cgtcatcaga ttccaccatt gcgatagcct gatgcacatt cgggtccagc
    18841 ggcacgttag tttcggcgat cacttcaaca ccaaacttac gcacaacatc cagcatcgac
    18901 ttcagcgtca gctcaatgcc ttcaaccatc gcggacatat ccgggttagc tttatcagcc
    18961 acttccagcg cacgatccag gctatcaatc accggcagca attcgttgat gaatttctcc
    19021 agcgcgaatt tgtgggcttt ttcaatatcc agttcagtac gtcgacgcag gttttccatt
    19081 tcggctttta cacgcaaaat gccgtcacgt tcacgggtct gggcttcagc cagctgagct
    19141 tcgagattcg caattttttc atcgcgcggg tccacctgct cagcagaagc ttctggctca
    19201 actgcctcaa tctcttcgtg ctgatccatg ataatttctt ccggggcttg cccctcaggc
    19261 gttttctgtt ctttactact catgaatttc tccgcgtttt tttcgcattc atctcgctaa
    19321 cttcgcttat tatggggatc agtttcaggg tttcaaggga agcactcaca ttgtcatcaa
    19381 tcttcgcaac aaggacctcg gaaaaatgaa taatcatttc aagtgtattg gcattgtggg
    19441 acacccacgg caccccactg cactgacaac acatgaaatg ctctaccgct ggctgtgcac
    19501 aaaaggttac gaggtcatcg ttgagcaaca aatcgctcac gaactgcaac tgaagaatgt
    19561 gaaaactggc acgctcgcgg agattgggca actagctgat ctcgcggtag tcgttggtgg
    19621 cgacggtaat atgctgggcg cggcacgcac actcgcccgt tacgatatta aagttattgg
    19681 aatcaaccgt ggcaacctgg gtttcctgac tgaccttgac cccgataacg cccagcaaca
    19741 gttagccgat gtgctggaag gccactacat cagcgagaaa cgttttttgc tggaagcgca
    19801 agtctgtcag caagattgcc agaaacgcat cagcaccgcg ataaatgaag tggtgcttca
    19861 tccaggcaaa gtggcgcata tgattgagtt cgaagtgtat atcgacgaga tctttgcgtt
    19921 ttctcagcga tctgatggac taattatttc gacgccaaca ggctccaccg cctattccct
    19981 ctctgcaggc ggtcctattc tgaccccctc tctggatgcg attaccctgg tgcccatgtt
    20041 cccgcatacg ttgtcagcac gaccactggt cataaacagc agcagcacga tccgtctgcg
    20101 tttttcgcat cgccgtaacg acctggaaat cagttgcgac agccagatag cactgccgat
    20161 tcaggaaggt gaagatgtcc tgattcgtcg ctgtgattac catctgaatc tgattcatcc
    20221 gaaagattac agttatttca acacattaag caccaagctc ggctggtcaa aaaaattatt
    20281 ctaattttac gccagcctct ttactgtata taaaaccagt ttatactgta cacaataaca
    20341 gtaatggttt ttcatacagg aaaacgacta tgttggcaca actgaccatc agcaactttg
    20401 ctatcgttcg tgagcttgag attgattttc atagcggcat gaccgtaata actggcgaga
    20461 ccggcgcggg taaatctatt gcaatagatg ccctcggtct ttgtctcggt ggtcgcgctg
    20521 aagccgacat ggtgcgtacc ggcgctgctc gcgctgacct gtgcgcccgt ttttctctga
    20581 aagatacgcc agcggctctg cgctggctgg aagaaaacca gcttgaagac gggcatgaat
    20641 gtttgcttcg tcgcgtgatc agcagcgatg gtcgctcccg tggtttcatc aacggtacag
    20701 ctgttcctct gtcacaactg cgcgaactgg gtcagttgct gattcagatc catggtcagc
    20761 acgctcatca gttactcacc aaacccgagc accaaaagtt cctgcttgat ggctatgcca
    20821 atgaaacctc tctactccag gaaatgacag cacgttatca gttgtggcat caaagctgcc
    20881 gtgacctcgc gcatcatcaa cagttaagtc aggaacgcgc tgcccgtgcg gaactgctgc
    20941 aataccaatt aaaagaactt aacgaattta atccgcagcc cggagagttt gaacaaatcg
    21001 acgaagagta caaacgtctg gcgaacagcg gtcaattgct gaccaccagc cagaatgcat
    21061 tggcattaat ggccgacggt gaagacgcaa acctgcaaag tcagctttac acggctaaac
    21121 aactggtgag cgaattgatt ggcatggaca gcaaactgtc cggcgtactt gatatgctgg
    21181 aagaagctac catccagatt gctgaagcca gcgatgaact gcgccactac tgcgatcgtc
    21241 tggatctcga tcccaaccga ctatttgaac ttgaacagcg catctcaaaa cagatttcgc
    21301 tggcacgtaa acatcacgtc agccctgagg cattgccaca gtattaccag tcgctactgg
    21361 aagaacagca gcaactggac gatcaggccg actcacaaga aacgcttgcg ctggcggtaa
    21421 cgaaacatca tcagcaggca ctggaaatcg cgcgcgcatt acaccaacaa cgccagcaat
    21481 atgcagaaga acttgcacag ctgatcaccg acagtatgca tgcgctctca atgccgcatg
    21541 ggcagtttac gatcgatgtt aaatttgacg agcatcacct gggcgctgac ggtgccgatc
    21601 gtattgagtt tcgggtaacc accaacccag gtcagccaat gcagcctatt gccaaagtcg
    21661 catccggtgg tgaattgtcc cgcatcgcac tggcaatcca ggtcatcacg gcgcgtaaaa
    21721 tggaaacccc ggcactgatt tttgatgaag tggatgtagg gattagcggt ccaacagcgg
    21781 cagttgtcgg caaactgctg cgtcaactcg gcgaatcaac tcaggtgatg tgtgttaccc
    21841 acctgccaca agtcgcggga tgtggtcatc aacactattt tgtcagcaaa gaaaccgatg
    21901 gtgcgatgac agaaacgcat atgcaatccc tgaataaaaa agcgcggtta caagagctgg
    21961 cgcgcctgct tggtggcagt gaagtcacac gtaatacact ggcgaatgcg aaagaactgc
    22021 ttgcagcgta aacttttttc ctgcttcacg gtcagagtaa acagcaaaac gccgtaagac
    22081 cggaaagcaa aaggttttaa agtgatgaaa ggtctattat catcggcata ttacagatga
    22141 gccacgtact gctcgggccc gaaaaggaat caaatcacta tgcgctgtaa aacgctgact
    22201 gctgcagcag cagtactatt gatgttgacc gcaggctgtt ccactctgga gcgagtggtt
    22261 taccgtcctg acatcaacca ggggaactat ctgaccgcta acgacgtatc caaaatacgt
    22321 gttggcatga cgcaacaaca agttgcgtac gcattgggta caccgctgat gtccgatcca
    22381 tttggtacga atacctggtt ctatgtcttc cgccagcaac caggtcatga aggtgtaacg
    22441 cagcaaacgc tgacgctgac ctttaacagt agcggtgtgt tgaccaatat tgataacaaa
    22501 cctgcgctga gtggtaacta ataataaagt tgctctcaaa gacgttaaaa aaggtgctca
    22561 atgagcacct tttttctgtc tgttatttat tcgctgattt ttctgctcgt tgcctgcgaa
    22621 gctctttcgg atcggcaatg agagggcgat aaatctccac ccgatcgcca tcatgcacac
    22681 tatcgcttag ttttgccgga cggctgtaaa tgccgacttt gtttttagtt aaatcgatat
    22741 cggtacgcaa ttccagcaag ccactggcgc gaatagcttc ttcaaccgtc gcgccctcct
    22801 gcagcgtcac tcgctgcagg tactgcttct caggtagcgc ataagccacc tcaacggcaa
    22861 ttttacctgg cactgtaaac ctctttcgca cgaaccgtaa aagcctggac catattagcc
    22921 gccagctctt taaacacgcg accaaaggcg agttcaatca acttattggt aaactcaaag
    22981 tcgagatgaa actcgatacg acacgcctcc tggctcagcg gcgtaaactt ccatccacca
    23041 atcaatttct tgaacggccc atccaccaga ttcataagaa tactttggtt actggtcaac
    23101 tggttgcggg tagtaaacgt tttgctgatc ccagccttag agacatctac cgccgcagtc
    23161 atctgcccag gagtggactc cagaatccga cttccggtac aacccggcaa aaactgagga
    23221 taagactgaa cgtcattcac taactgatac atttgctccg cgctgtaggg taccagtgcg
    23281 gtccggctaa tctgaggcat aacaatttcc atcaacaaaa atccaacaaa taatatcatt
    23341 tatcctgctg taaaaaaaac gctatcccgg cgctgggtaa catcgggttc atgctaagat
    23401 agagccttgt cccccgcagg attgatatgg ggtgttttcg atttcagatt accgatgatt
    23461 cacgacgctt atgacgaaga aaaaagcaca taaacctggt tcagcgacca tcgcgcttaa
    23521 caagcgcgcc cgtcacgaat actttatcga agaagagttc gaagcgggac ttgccctgca
    23581 aggctgggag gttaaatccc tgcgcgcagg aaaagccaat atcagcgaca gctacgtcct
    23641 tctgcgtgac ggagaggcat ttctgtttgg cgctaacatc acgccaatgg ccgtggcctc
    23701 cacgcatgtg gtgtgcgatc ctacccgtac ccgcaagtta cttctcaacc aacgcgaact
    23761 ggactcattg tacggtcgcg tcaatcgaga aggctatacc gtagtggcgc tctccctgta
    23821 ctggaaaaat gcctggtgca aagtgaaaat cggcgtcgcc aaaggtaaaa aacagcacga
    23881 taaacgttca gatatcaaag aacgcgaatg gcaggtggat aaagcacgta tcatgaaaaa
    23941 cgcccaccgt taaacctgca ctccaattat tgaccagttc ctcaccgcgc ctccctctcc
    24001 ggcggcgcga atgaacatct tattggctat cacatccgac acaaatgttg ccatcccatt
    24061 gcttaatcga ataaaaatca ggctacatgg gtgctaaatc tttaacgata acgccattga
    24121 ggctggtcat ggcgctcata aatctggtat acttaccttt acacattggg gctgattctg
    24181 gattcgacgg gatttgcgaa acccaaggtg catgccgagg ggcggttggc ctcgtaaaaa
    24241 gccgcaaaaa atagtcgcaa acgacgaaaa ctacgcttta gcagcttaat aacctgctta
    24301 gagccctctc tccctagcct ccgctcttag gacggggatc aagagaggtc aaacccaaaa
    24361 gagatcgcgt ggaagccctg cctggggttg aagcgttaaa acttaatcag gctagtttgt
    24421 tagtggcgtg tccgtccgca gctggcaagc gaatgtaaag actgactaag catgtagtac
    24481 cgaggatgta ggaatttcgg acgcgggttc aactcccgcc agctccacca aataatgatc
    24541 cggatacgtc cggtgaagta cagaaagccc gcatggcaca agccctgcgg gcttttttgt
    24601 gtttgtcgtt gtccgagaac atccggctaa atccgatgat tattggtata cgtttcggta
    24661 tacggtagga tgtataccta aaagcgtata ccaattcatg aaggagcggc cacagtggca
    24721 cggacaacac gccccctgac caacaccgaa gttctgcgcg cgaaagcgtt agagaaggat
    24781 ctaacgctgc atgatggcga tgggcttttc ctgatagtga aaactagcgg caagaaactc
    24841 tggcgctttc gctatcaacg cccagcaaca aaacagcgga ctatgatggg acttggtgct
    24901 tttcctgccc tttctcttgc tgatgcccga gggttaagag cagattacct tgccctatta
    24961 gccaacggaa tcgacccgca aattcaagct gaagttgcag aggaacagca gcaaatcgct
    25021 ctggacagta ttttttcgac ggtcgccgct aactggttcc agctcaaaag caaaagcgtt
    25081 acccatgatt acgctaaaga tatatggcgt tcactggaaa aagacgtatt ccccaccatc
    25141 ggggagatcc ctgtccagca aatcaaagcc cgaacactgg ttgaagccct tgaaccgatc
    25201 aaagctcgtg gggcgctgga gacggtgcgc cggttggtgc agcgtgttaa cgagataatg
    25261 atttatgccg taaatactgg tcttattgat gccaatccag catcaggcat tggaatggcc
    25321 tttgagaagc caaaaaaaca aaatatgcct acgttgcgcc cagaagaatt gcctatgctg
    25381 atgcgttctc tcgtcatgtc aaatttgtct gttccgactc gctgcctcat tgagtggcaa
    25441 ctcctgactc tagttcgtcc ttctgaggct tccggtgcta ggtgggctga gctcgatctc
    25501 gatgcaaagc tctggactat cccagctgaa cgaatgaagg ctaagcggga acacatagtc
    25561 cctctatctc ctcaggcatt agagattctt gatgtgatga agctaatcag tgcccatcgt
    25621 gaacatgttt tcccgagtcg gaatgaccca aagcaaccaa tgaatagcca gacggcaaat
    25681 gctgctttaa aaaggattgg atttggtggt agattggttg ctcatggatt gcgttcaatt
    25741 gccagcaccg cattaaatga agcgggtttt aatgcagatg taattgaggc tgctcttgca
    25801 catagtgata aaaatgaagt gcgcagagca tataatcgtt caacttacct tgagcaacga
    25861 ataggactta tgaattggtg gggccaaaaa gtgtacgagc gcaatcaata ttagttaact
    25921 tgttatacat ggagatatag ttcatggaga ataatctaga taatttatgt tatgaattct
    25981 ttagagagtt ttcacgttat gaatattgcc ttaaggcatg tggacttcat cataaaataa
    26041 aagatgctaa agcgaactgg gatgagtatg caaagcaagt ttctgaaatc ataaataaca
    26101 caactgatcc agagctaatt gctgctatca cctactttaa agaacaccca cccaaaaaac
    26161 aaatcataga agatgactct ttagtttggg atgattcttt accacaagaa aaagatttag
    26221 caaaatttct ttttttgtta atccgccgtg taagaaacaa tctttttcac ggaggaaaat
    26281 ttaacgggaa atggtttgaa cctgaacgta gtgaggcgtt actgcaacat ggccttgtta
    26341 ttttgaggca ttgcggcaca aggcatagtg aagtaagcaa agcctattct ggaatagcct
    26401 gagcggtgtt ataaaagaga agtttttgtt actggaagaa tgataaaatt caaaaacttc
    26461 tctattgatt gtttaaaaaa tcgtctaaat aagactattg tacccaaaag ttacgaaata
    26521 tattatgtat ttcgttttga actaattgtg gcagttcttc catgtcaact ttattgtcct
    26581 taatccaatt aatgcattct tcctcatcta aactatcatt aggaataagc tctgaaatca
    26641 ttacagcagc agttcttgaa aagccaagcc ctattagtga aatctgagtt tttaaactta
    26701 cacccagttc taaataaaca ccgatatctt caacatcttt tgctaagtct gtgcgatcta
    26761 tcgaaagata aaagcttttt aataaatcat tataacatcc taaaagcctt ggagcttgat
    26821 agcgagcgat ctcttcaaca tccttcataa cccctcgtat taaagtcgct tctttgaaaa
    26881 caatattttt ctttttgtta tatcttattc tatccgagat caatcttgct aggggataac
    26941 ctttcatcca cttggaaact aacaaggcct gtacgtaagc tccttttgaa ttataaccta
    27001 gcttcttact cagagtatca ctaattctag agaatgcgga tacatatgaa tctaacgcat
    27061 cattactacc aggttcagaa agcagtaatc tctctacagg tttgtttgta tcccgcgaaa
    27121 atctatccca aaggttttgc attaaaagcg ggcttatacc aggatgccgc acaaccaatt
    27181 cgtgtggaaa agttaaagaa t
//
LOCUS       DAJOWB010000058        26887 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJOWB010000058 DAJOWB010000000
VERSION     DAJOWB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.058"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            4..237
                     /locus_tag="OHO85_002598"
     CDS             4..237
                     /locus_tag="OHO85_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCQ2671957.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            337..1155
                     /locus_tag="OHO85_002599"
     CDS             337..1155
                     /locus_tag="OHO85_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCQ2671958.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            1237..1716
                     /locus_tag="OHO85_002600"
     CDS             1237..1716
                     /locus_tag="OHO85_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCQ2671959.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFSAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            1732..2208
                     /locus_tag="OHO85_002601"
     CDS             1732..2208
                     /locus_tag="OHO85_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCQ2671960.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            2271..2492
                     /locus_tag="OHO85_002602"
     CDS             2271..2492
                     /locus_tag="OHO85_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCQ2671961.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DITGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            2566..2934
                     /gene="cbtA"
                     /locus_tag="OHO85_002603"
     CDS             2566..2934
                     /gene="cbtA"
                     /locus_tag="OHO85_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HCQ2671962.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            3024..3398
                     /locus_tag="OHO85_002604"
     CDS             3024..3398
                     /locus_tag="OHO85_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCQ2671963.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            3395..3589
                     /locus_tag="OHO85_002605"
     CDS             3395..3589
                     /locus_tag="OHO85_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2671964.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            3602..3715
                     /locus_tag="OHO85_002606"
     CDS             3602..3715
                     /locus_tag="OHO85_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HCQ2671965.1"
                     /translation="MQGIVPLTTETALLSCIKQAREDVLTLRHLQLLHQNR"
     gene            complement(4004..4132)
                     /locus_tag="OHO85_002607"
                     /pseudo
     CDS             complement(4004..4132)
                     /locus_tag="OHO85_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            4204..4386
                     /locus_tag="OHO85_002608"
     CDS             4204..4386
                     /locus_tag="OHO85_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCQ2671966.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(4487..4816)
                     /locus_tag="OHO85_002609"
     CDS             complement(4487..4816)
                     /locus_tag="OHO85_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCQ2671967.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(4988..6046)
                     /locus_tag="OHO85_002610"
     CDS             complement(4988..6046)
                     /locus_tag="OHO85_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCQ2671968.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(6245..6718)
                     /gene="sbmC"
                     /locus_tag="OHO85_002611"
     CDS             complement(6245..6718)
                     /gene="sbmC"
                     /locus_tag="OHO85_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HCQ2671969.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(6837..8003)
                     /gene="dacD"
                     /locus_tag="OHO85_002612"
     CDS             complement(6837..8003)
                     /gene="dacD"
                     /locus_tag="OHO85_002612"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HCQ2671970.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            8212..9639
                     /gene="sbcB"
                     /locus_tag="OHO85_002613"
     CDS             8212..9639
                     /gene="sbcB"
                     /locus_tag="OHO85_002613"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCQ2671971.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAACIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(9682..9909)
                     /locus_tag="OHO85_002614"
     CDS             complement(9682..9909)
                     /locus_tag="OHO85_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCQ2671972.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(9923..10981)
                     /gene="yeeE"
                     /locus_tag="OHO85_002615"
     CDS             complement(9923..10981)
                     /gene="yeeE"
                     /locus_tag="OHO85_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCQ2671973.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYLAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(11160..12518)
                     /gene="plaP"
                     /locus_tag="OHO85_002616"
     CDS             complement(11160..12518)
                     /gene="plaP"
                     /locus_tag="OHO85_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCQ2671974.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(12508..12570)
                     /gene="yoeI"
                     /locus_tag="OHO85_002617"
     CDS             complement(12508..12570)
                     /gene="yoeI"
                     /locus_tag="OHO85_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCQ2671975.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(12785..13714)
                     /locus_tag="OHO85_002618"
     CDS             complement(12785..13714)
                     /locus_tag="OHO85_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2671976.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(13760..14584)
                     /locus_tag="OHO85_002619"
     CDS             complement(13760..14584)
                     /locus_tag="OHO85_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCQ2671977.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLRAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(14667..14921)
                     /gene="yoeB"
                     /locus_tag="OHO85_002620"
     CDS             complement(14667..14921)
                     /gene="yoeB"
                     /locus_tag="OHO85_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCQ2671978.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(14918..15169)
                     /gene="yefM"
                     /locus_tag="OHO85_002621"
     CDS             complement(14918..15169)
                     /gene="yefM"
                     /locus_tag="OHO85_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCQ2671979.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            15452..15502
                     /gene="hisL"
                     /locus_tag="OHO85_002622"
     CDS             15452..15502
                     /gene="hisL"
                     /locus_tag="OHO85_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCQ2671980.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    15478..15601
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            15648..16547
                     /gene="hisG"
                     /locus_tag="OHO85_002623"
     CDS             15648..16547
                     /gene="hisG"
                     /locus_tag="OHO85_002623"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCQ2671981.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            16553..17857
                     /gene="hisD"
                     /locus_tag="OHO85_002624"
     CDS             16553..17857
                     /gene="hisD"
                     /locus_tag="OHO85_002624"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCQ2671982.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGNEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQEMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLASAERLTAHKNAVTLRVNALKEQA"
     gene            17854..18924
                     /gene="hisC"
                     /locus_tag="OHO85_002625"
     CDS             17854..18924
                     /gene="hisC"
                     /locus_tag="OHO85_002625"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCQ2671983.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIIAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            18924..19991
                     /gene="hisB"
                     /locus_tag="OHO85_002626"
     CDS             18924..19991
                     /gene="hisB"
                     /locus_tag="OHO85_002626"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCQ2671984.1"
                     /translation="MSQKYLFIDRDGTLISEPPGDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            19991..20581
                     /gene="hisH"
                     /locus_tag="OHO85_002627"
     CDS             19991..20581
                     /gene="hisH"
                     /locus_tag="OHO85_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCQ2671985.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            20581..21318
                     /gene="hisA"
                     /locus_tag="OHO85_002628"
     CDS             20581..21318
                     /gene="hisA"
                     /locus_tag="OHO85_002628"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCQ2671986.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKNLVAGVNVPVQVGGGVRSEDDVAALLEAGVARVVVG
                     STAVNSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            21300..22076
                     /gene="hisF"
                     /locus_tag="OHO85_002629"
     CDS             21300..22076
                     /gene="hisF"
                     /locus_tag="OHO85_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCQ2671987.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLDDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDETRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGDLKAYLATQGVEIRIC"
     gene            22070..22681
                     /locus_tag="OHO85_002630"
     CDS             22070..22681
                     /locus_tag="OHO85_002630"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCQ2671988.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVNDRFELKNEASDLVYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(22777..23435)
                     /gene="wzzB"
                     /locus_tag="OHO85_002631"
                     /pseudo
     CDS             complement(22777..23435)
                     /gene="wzzB"
                     /locus_tag="OHO85_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
     gene            complement(23490..23747)
                     /locus_tag="OHO85_002632"
                     /pseudo
     CDS             complement(23490..23747)
                     /locus_tag="OHO85_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23913..25079)
                     /gene="ugd"
                     /locus_tag="OHO85_002633"
     CDS             complement(23913..25079)
                     /gene="ugd"
                     /locus_tag="OHO85_002633"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCQ2671989.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDILNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKLIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GEHSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDFATFKQQADVIISNRMAEELKDVADKVYTRDLFGCD"
     gene            complement(25326..26732)
                     /gene="gndA"
                     /locus_tag="OHO85_002634"
     CDS             complement(25326..26732)
                     /gene="gndA"
                     /locus_tag="OHO85_002634"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCQ2671990.1"
                     /translation="MSQQHIGVYGMAVMGSNLALNIESRGYTVSIFNRSREKTEEVIE
                     GNPGKNLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFLDTIRRNRELSAEGFHFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRYVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT         6849 a         6983 c         6623 g         6432 t
ORIGIN      
        1 gatatgccgg gttacacaga gtatgtcctg gctgaaggat ctttctcata cgggcaggcg
       61 gttgctgtca taaccgctta ccgcaatgtg tttatccagg atgacccggg aatgcatttc
      121 cgtcgggtta tccgcaacgc ggaaggtcag cgacgctggc gctgccggaa ttctgaaccc
      181 gatgccggaa aggtgcttaa tacgcggctc gccagtgacg gtcttctcag acaataaacg
      241 tcattcattt cacccatcag gccgcgtctt ctccgggaga cgtggctttt tcatttatta
      301 caccagtaaa tcttaaccac cgataaggag caaattatgc gactagccag tcgttttggt
      361 tatgcgaacc agatacgccg tgaccgtccg ctgacacacg aagaactgat gcactatgtg
      421 ccggggattt tcggggaaga taaacacacg tcccgaagcc agaactatac gtacatcccc
      481 accatcaccg tactggaaag cctgcagcgg gaaggctttc agccattctt cgcctgccag
      541 acccgtgtgc gcgacccggg ccgccgggga tacacaaaac acatgctgcg tctgcggcgg
      601 gccggagaga taaacggaga acatgtccct gaaattattc tgctcaactc tcatgacggt
      661 acctccagct accagatgct gccggggtac ttcaggttcg tctgccagaa cggctgcgtc
      721 tgtggtcagt ctctggggga agtacgtgtt ccgcaccggg gaaatgtggt ggacagagtc
      781 attgaagggg cttacgaggt ggtgggcgtg tttgaccgga tagaggagaa gcgtgatgcc
      841 atgcagtcgc tggtcctgcc gccaccggca cgccaggcgc tggcacaggc ggcactgact
      901 taccgttatg gtgacgaaca tcagcccgtc accaccgccg acattctgac gccgcgacgc
      961 cgggaggatt acggtaagga cctgtggagc gcatatcaga ccatccagga gaatatgctg
     1021 aaaggcggga tttccggtcg cagtgcaaaa ggaaaacgta tccatacccg ggccattcac
     1081 agcattgata ccgacattaa gctcaatcgc gcattgtggg tgatggcaga aacgatgctg
     1141 gagagcctgc gctgatgccg tttccctgag tgagggaatc attcagcttt cgttcctgaa
     1201 aaacacacat ttatttgtta tttaaggagt tatctcatga aaaccgtttc tcagaatacc
     1261 cccacaattt attcagctac aacaccagag aataatccgc ctcagttggt tgccagcctc
     1321 gtccctgatg aacagcgcat cagcttctgg ccgcagcatt ttggcctcat tccacagtgg
     1381 gtgaccctgg agccccgtgt cttcggctgg atggaccgtc tgtgcgaaga ctactgcggt
     1441 ggtatctgga atctgtacac cctgaacaac ggcggggcat ttatggcacc cgaaccggat
     1501 gacgatgatg acgaaacatg ggtactgttc agtgccatga acggtaaccg cgcagaaatg
     1561 agcccggaag ccgccggtat tgccgcctgt ctgatgacgt acagtcatca tgcctgtcgt
     1621 acggagaatt atgccatgac ggtccattat taccggttgc gggattacgc cctgcagcat
     1681 ccggaatgca gcgccattat gcgcatcatc gactgaatgg agagaagcac aatgcaacaa
     1741 ctttcctttc tgcccggaga gatgacgccc ggcgagcgca gcctcattct gcgggctctg
     1801 aaaaccctgg accgccatct tcatgaaccc ggcgtggcct tcacctccac ccgtgcagca
     1861 cgggaatggc tgattctgaa catggcggga ctggagcgtg aagaattccg ggtgctgtat
     1921 ctgaacaacc agaatcagct gattgccggt gaaaccctct tcaccggcac catcaaccgc
     1981 acggaagtcc atccccggga agtgattaaa cgcgccctgt accacaatgc cgctgccgtg
     2041 gtactggcac acaatcaccc gtccggtgaa gtcacaccca gtaaggcaga ccggcttatc
     2101 accgaacgtc tggtacaggc actgggcctg gtggatatcc gggtgccgga ccatctgata
     2161 gtcggtggca accaggtttt ctcctttgcc gaacatggtc tgctttaacc cttcacaacc
     2221 acatcacacc tgttttcact tttatcttct gtcttcagag gtatcccatt atgaaaatta
     2281 tcacccgtgg tgaagccatg cgtattcacc aacaacatcc gacatcccgt ctttttccgt
     2341 tctgtaccgg taaataccgc tggcacggca gtgctgaagc gtataccggt cgtgaagtgc
     2401 aggatattac cggtgtgctg gccgtgtttg ctgaacgccg taaggacagt tttggtccgt
     2461 atgtccggct gatgagcgtc accctgaact gagtgggaat tctgatgagc agaattatcg
     2521 ccactaccgg accattctta gccgattttc tgtaaggatt ttatcgtgtc agacacactc
     2581 cccgggacaa cacttcccga cgacaatcac gaccgcccct ggtggggact accctgcaca
     2641 gtgacgccct gtttcggcgc ccgtctggtg caggagggta accgcctgca ttaccttgcc
     2701 gaccgcgccg gtatcagagg cctgttcagc gatgcagatg cgtaccacct ggaccaggcc
     2761 tttccgctgc tgatgaaaca actggaactc atgctcacca gcggtgaact gaatccccgc
     2821 catcagcata ccgtcacgct gtatgcaaaa ggtctgacct gcaaagctga cactctcggt
     2881 agcggtggtt acgtttatct ggctgtttat ccgacgcccg aaacgaaaaa gtaaaccacc
     2941 ggaataacct tctgctccgg cctggtgctt tcaccacgcc acttttccat ttttcatctc
     3001 tgcatatcag gaaaatcttc agtatgaaaa cattacccga tacacacgta cgggaggcat
     3061 cgagctgccc gtctcccgtc accatctggc agactctgct ctcccgacta ctgggccagc
     3121 attacggcct tacactgaat gacacaccgt tcgccgatga acgtgtgatt gagcagcata
     3181 ttgaagctgg catttcactg tgtgatgcgg tgaattttct cgtggaaaaa tacgcgctgg
     3241 tgcgtaccga ccagccggga ttcagcgcct gtacccgctc tcagttaata aacagtattg
     3301 atatcctccg ggcacgccgg gcaaccggcc tgatgacccg cgacaattac agaacggtaa
     3361 ataacattac cctgggtaag tatccggagg cgaaatgatg acactggaag ccgacagcgt
     3421 taacgtacag gcgctggata tggggcacat tgtcgttgac attgatggtg ttaatatcac
     3481 tgaactgatt aataaggccg ctgaaaacgg ttattcactc cgcgtggtgg atgaccgtga
     3541 ctctaccgaa acaccggcaa cttatgccag ccctcaccag ttgctgtaag acaatgcagt
     3601 gatacagggt atagtgccat taaccacaga aacagcactg ttgtcctgta ttaagcaggc
     3661 aagagaagat gtccttaccc tgcgccatct gcagcttctg caccagaacc ggtgaatctc
     3721 actcaacagg cttcatttct cctgacgcca gcctgaatac agctggcgtt ttcatttata
     3781 aacagaaagg aaaaccgata ataatggaat ggacgccccg accatgaagc aacggtagga
     3841 tattactgat aaaccttcca gtggaggtgc gttatggccg ttttaacgat tagcattgac
     3901 ctgacaaaaa atgaattcca aatccacggt ctgggtagga acagaaaaat ctaagctcag
     3961 aaaacggatt aaagtaagcg tcaacggagc accgtattga cgcttattta ttggtgagaa
     4021 ctacgttcca tggtaggagt tcgtcaacac ggttggaggg ccattccggc agtatgctca
     4081 gaatatggcg aagatacgct tccggatcga taccgttcag acggcaggtg cctgcttttc
     4141 tggcaacaag caggaatctc agcgctgaag caggaattag aggtaaaaac gccctaccgg
     4201 gccatgaatc acccggtcat tggagtagta accaaagcag atttagccag catggaacaa
     4261 atatctttgg tgaaaagttg gttacgggaa gctggagcgc acaacgtgtt agtaaccagt
     4321 gcagttaata acaatggagt tacagaactt tttgccttgc tgcatacaga agaaggctgt
     4381 tgttaatttc gattggttct cggatgagaa tgatgacggt gatacacaga taagaatcaa
     4441 tgagcaatga gggttgccgg gcaaccctca ttgaataaaa cgggaattac ttcgcttcgc
     4501 cgtttttcat ttcgcccata gctttcagct ttttggagat atcgcggcgt tctttggaga
     4561 gctcggcatt tttgatgatg taatcgtcaa cgcgatcttc atagtcacct ttcatgctgg
     4621 cgatgatgcc ctggattgct tcaacgctca tccccggctt gatgtaatcg ctcaggttgt
     4681 ccagcagcag gacgcgcttc tggttgtcac ggatcttttt ctcaacgtcc tgaatttcac
     4741 gttgcagttt gttcttacga cggaacagac gaacaaattc cagtacgtcc tggaatgaag
     4801 gcttggtagt ttccatttgt atacccctga taatgtgaga gtcggattcg tttaagcaac
     4861 cgctattcgt tagggccaac attactcata gctgccgcag atgacaatgc ttttatccct
     4921 tttcactatc atacccttta tccttgctga atcgaagcag cagcaagatg attctgaagt
     4981 tcaggaatta tttgcgcaag gcccggcaaa tcagattcga tagcaactca agctgatgtg
     5041 ccgtttccat gttcagccac acataaccat agattggtgt ttccactacc ttcaggtcat
     5101 ggtggttatt gagcaactga cgcagctctt ccacagcatc gttcaatttt tccgtattgg
     5161 caaaaaccgg ctgcggatta ccttcgtaca gcgcatgaac aaggctcagc agtatttgct
     5221 gcatcatgtg ctgggtatca cgaagttttt gcgcgttcaa taacacgaaa tggctggggc
     5281 gcgtggccca gtacgcattg atttgcaact ccagcataca aaccagattg cggttaatgg
     5341 tctggatacc ttcatatatc gattttggaa tacgggtttc tttgctggcg ggcgcaatca
     5401 gcccacgcat tttcacggca tcggtcagga gtttttgtag atggctttcc agacgtgggc
     5461 gttcgagtaa gttcggtgag aatgcagatt gatagacccg attatactcg gtcagacttt
     5521 tcgccagttg aatgcgccag tggatgaacg cccgttgtgg ccagataccg gtaaacaaca
     5581 ttgccagtaa agagccgagg atcacatcgc cgcttcgcca taacgccgta tcaatttcac
     5641 ctgtcgggga accaacaaca attgccagcg tcaccccaat caataaacct tgatacggtt
     5701 tcttgcccag cgccagccaa ccgcaaagga acatggccgc cgcgcaccag actaacatca
     5761 gcggtaacga gattaactcc agttgcagag cgataagacc taaaatcgaa cccaacaccg
     5821 taccgccaat acgctcaaag gcgcgaggga caacgttacc ccagaacgag attggcccca
     5881 taatcaccac catggtgacc agcggccagg tgctttccgg gatagtaaac aggcggataa
     5941 tgagaaaagt gagcaggaat gccagcgcga cccgagtacc atgcacaatg cggtaatggc
     6001 ggtatacccg gatttcaaac gggcttaatg acttatcggc acgcacgcag aaactccgta
     6061 atgaagatta aaagcgctca taacacatct gtcggccaga aggcgcgcct ctgagttatc
     6121 cgacggacat accgggaaca gtgtaatttc ccggcaggag cgaaaaaatt gaaaggcgca
     6181 tcttactctt ttcgctttca aaaaaaagag tggtcatcgc gttaacacac cgccctgaga
     6241 tgaattagtg atgttttggc tgcaccgcaa catacatttc gatatcccag tacccatctt
     6301 ccgcgccatt gttcaaataa acctcgaagc agggctttgg taacatttca taagcactgt
     6361 cctgcaagag gctattaaag aactgatacc agggtttagc aaaatcatca ccgactacac
     6421 gagccaccgc aaccgcatac tgaccacctg aaatttctgt cagaatgacg ccctcactgt
     6481 tttcgggaag cgtaaagtta ttcggtaccg tcacaacggt gtcgcagcgt aatttttcgg
     6541 cgggtgtttc atctggattg tcgtaataga cagcaaccca ctccttcggc acaatatttt
     6601 tgttatctac ccacatcatc aactgctcaa agcctttctt taccgtctgt tcccacgggc
     6661 caacgagatg gaaacctgca acggtacgtt tctcttcctg cttaatctcg tagttcatga
     6721 cgcctccatt aacactgttt ttatatacag tatagttgca atattaaaac cacaaggaat
     6781 gagtgttgat tatgcgagca gactcgcact cctgccagtc tgctgcaaaa gaaaggtcag
     6841 gccttatggt ggaaataatc actcaggcgg gaaaacatgc cgccttcccc gacagattcc
     6901 agggtaacca gcggccagtg cgccacctgt ttatcacggt cgtaaagttc aatttcccct
     6961 acccgctgat gggcgctaat tggcgcggtg agctctttac catcaagggt atatttggct
     7021 ttgatatgtg gaatttcggc tttcggtagc accatccaga actcttgttc cgttcccagg
     7081 tcgatatttt ctttatcgcc ataccagatg cgttccgttc cgaccttttt cccacggtgc
     7141 aaaatttgca ccgtagtaaa gttttgttgc ccccaacgca gtaattttct tgcctcttcc
     7201 tcacgacctt ttgcactgtc agcccccatt accactgcaa tgagacgacg ctgcccatct
     7261 acagccgaag caatgagatt aaacccggca ccagaagtat gacccgtttt caggccgtca
     7321 acattcatgg ttttatccca caacaacccg ttacggtttt gctgggtgat accgttccag
     7381 gtgagacttt tctcactgta catatgataa aactcgggct cgccgtggat gatagcgcga
     7441 gaaagcacag ctaaatcata agccgagcta tgctggccag gtgcatccag accatgcact
     7501 gtttcaaaat gcgtatcctt gagatgcagc ttctcggcat agttgttcat catttcaaca
     7561 aactgccgtt gcccaccggc aatatagtca gccagagcaa cacaagcgtc atttccggaa
     7621 tccacaatta aaccacggct taaatcacgt accgatacgc gatcgccctc tttcaaaaac
     7681 atcagtgaag aaccgacaaa caccggatta tctttcgccc acgcatcgcg ccccacggtg
     7741 acaatatcgt ctggcgtaat gcgatgacta tcgatagcgc gatccacgac ataacccgtc
     7801 atcagctttg tcaggctggc gggattgcgc tgttgatgct cattacccgc ggtgaggatc
     7861 tgaccggtgg tgtaatccat caatacccag gacccggcat gaatctctgg aggctgaggt
     7921 gaaaaaggaa tgttttccgc cgcaaaacca gacgataagt taaaaacgaa caaagaagca
     7981 gcaataataa gacggcgttt caacagcaaa ccctcaggag tttcaaatag ctgttctttt
     8041 tacggaaata cttatgaact ggctggaata aagtgcaaga aaatgtgact accctctcat
     8101 ttttatctga catgatctgt tgccactcgc tgccaaattg tggcgctaaa gctgattagc
     8161 acggtgatat ttgatactct ggcagacagc agaaataacg gatttaacct aatgatgaat
     8221 gacggtaagc aacaatctac ctttttgttt cacgattacg aaacctttgg cacgcacccc
     8281 gcgttagatc gccctgcaca gttcgcagcc attcgcaccg atagcgaatt caatgtcatc
     8341 ggcgaacccg aagtctttta ctgcaagccc gctgatgact atttacccca gccaggagcc
     8401 gtattaatta ccggtattac cccgcaggaa gcacgggcga aaggagaaaa cgaagccgcg
     8461 tttgccgcct gtattcactc gctttttacc gtaccgaaga cctgtattct gggctacaac
     8521 aatgtgcgtt tcgacgacga agtcacacgc aacgtttttt atcgtaattt ctacgatcct
     8581 tacgcctgga gctggcagca tgataactcg cgctgggatt tgctggatgt tatgcgtgcc
     8641 tgttatgccc tgcgcccgga aggaataaac tggcctgaaa atgatgacgg tctaccgagc
     8701 tttcgccttg agcatttaac caaagcgaat ggaattgaac atagcaacgc ccacgatgcg
     8761 atggctgatg tgtacgccac tattgcgatg gcgaaactgg taaaaacgcg tcagccacgt
     8821 ctgtttgatt atctctttac ccatcgtaat aaacacaaac tgatggcgtt gattgatgtt
     8881 ccgcagatga aacccctggt tcacgtttcc ggaatgtttg gggcatggcg cggcaatacc
     8941 agctgggtgg caccgctggc gtggcatcca gaaaatcgca atgccgtaat tatggtggat
     9001 ttggcaggag acatttcgcc attactggaa ctggatagcg acacattgcg cgagcgttta
     9061 tatactgcaa aagccgatct tggcgataac gccgccgttc cggttaagct ggtgcatatc
     9121 aataaatgtc cggtgctggc ccaggcgaat acgctacgcc cggaagatgc cgaccgactg
     9181 ggaattaatc gtcagcattg cctcgataac ctgaaaattc tgcgtgaaaa tccgcaagtg
     9241 cgcgaaaaag tggtggcgat attcgcggaa gccgaaccgt ttacgccttc agataacgtg
     9301 gatgcacagc tttataacgg ctttttcagt gacgcagatc gtgcagcaat gaaaattgtg
     9361 ctggaaaccg agccgcgtaa tttaccggca ctggatatca cttttgttga taaacggatt
     9421 gaaaagctgt tgttcaatta tcgggcacgc aacttcccgg ggacgctgga ttatgccgag
     9481 cagcaacgct ggctggagca ccgtcgccag gtcttcacgc cagagttttt gcagggctat
     9541 gcagatgaat tgcagatgct ggcacaacaa tatgccgatg acaaagaaaa agtggcgctg
     9601 ttaaaagcgc tttggcagta cgcggaagag atcgtctaat ttgaagcctc cgccgctggt
     9661 acggcagagg acaatgttga gtcaggcttt ttgaacggtg atgctccacg ctgcatcgcc
     9721 aatctgctga taatcggtga tggcatgtcc ttcttctgcc gcccactgtg ggattgcttc
     9781 agttgcctgg gtgcaatcaa actcaattac caactcatcg ccgctggcca tttctgccag
     9841 tgctgctttt gcttctatga gtggaaacgg acagacctgc gtcaccacat ccagcttttt
     9901 aatcgccatc tttacacctt acttaatttg ccgcagcagt cgccagtcgc gccttacgct
     9961 gcggtcgaac atacacaagc caggacgcag tccagactcc gagaatcata aatacaaggc
    10021 caatccagcc ctgccaggtc atcattgcag tcataaccag tccattaccg atagagcaac
    10081 cacctgcaat actggcaccg aagcccatca gtacaccacc taacccgcta cgtaatgttg
    10141 tttgtgcatc agctgcgcga acgcggaact cacggctcgc tttggctgca ataaatgacc
    10201 ccacgaagat ccctaacacc aggaaaacgc cccagttaat gtatttcacg tcacccgcga
    10261 ccagaaattg cagaatattg gccgttgggg aagtgattcc taacccaaac atgcgtccgg
    10321 ttgcttcact caggggccag gctaaaagcg caatcaaacc gatgagtaca gcagtgacaa
    10381 agggatgcca gcgtttttca aacagaatat gagcgatccc ggttcggcgc ggcggtaagg
    10441 tcgcgacttt gagttttggc ttcttcaact ctttcatcac cacccagagc gttatcacca
    10501 gcaaaacggc aaccaacggc cacacagaca aattaaaagt ctcagcaata gagttatgtt
    10561 cagtactgta gtgctgcaag gtttgattta aaccactggc atgtggagaa cgcatcaccg
    10621 cactcatcac catataagtg aaaagcgcga tccaactgcc gatcaatccc tcacccgcgc
    10681 gataccaggt cccggtggca catccacccg ccagaacaat tcccagccca aagatatacc
    10741 cacctataac agtaccaagc cacgggaacg ccccggcttc gtaagtcagt aggcccgcct
    10801 gaattaacgc aaaaaccccc acgctttgta ccgaaattgc aatcagcaag gcgtaaaaca
    10861 tgcgattatt tttcgcgaga tacatatcgc gaaaaccacc tgtcaggcaa aaacgcccac
    10921 gctgcatgac aaatcccagc agagcaccac aaattagccc gcttaatatc attgaaaaca
    10981 taaattaatt aaccagatga atgttaatag ggaaattatt catgactggt ggaatgcaga
    11041 ccaataacaa aattctctaa attagaacaa atggttatta atgaggatgt cgataaaaaa
    11101 gaataaaaaa accggagcca aatattcatt cgactccggt ttattattac aaaagatgat
    11161 tacgctacgt cttcgtactg cggaaccggg ttacggaaac tcttggtgac gcaagccagg
    11221 taaatcagac cgatagccgc ccagatcaga cccagaacca ttgagctttc ttccaggtta
    11281 acccacagcg caccaacggt cagcgcacca cacatcggca ggaacagata ctggaagtga
    11341 tctttcagcg tcttgttacg cttctcacgg atccagaact gcgagatgac cgacaggtta
    11401 acgaaggtga acgccaccaa cgcaccaaag ttgatcagcg ctgtagccat taccaggtcg
    11461 aagttgattg ccagcaaggc aatcgcgcca accaggatga tgttcatcgc cggagtacgc
    11521 catttcgggt gtacataacc gaagaagctt ttcgggaata cgccgtcacg acccattacg
    11581 tacatcagac gcgcaacacc tgcgtgcgcc gccataccgg acgccagtac ggtaatggtg
    11641 gagaagatca gcgcaccaac ctggaatgct ttacctgcca cgtacagcat gatttcaggc
    11701 tgtgacgcat ccggatcttt aaagcgagag atatccggga agtacagctg caggaagtaa
    11761 gttgcaaaga tgaagatcat gccgccaatc agcgcggtca ggaaaatcgc acgcgggatc
    11821 acgcgctctg catctttggt ttcttccgac aggttgctga tgccgtcaaa gccggtaaag
    11881 gagaagcaca ggattgtcgc cccggtaatc atcgggataa catgcgcatc gccagaccag
    11941 aacggacgag tgctcgccag cgtaccagcg ccttcacctt caaatacgcc ataaacaacc
    12001 atgcccagaa tcaccgcgat cagcactacc tgcaacacga cgattacagt gttgaagttc
    12061 gctacggatt tcagactacg caggttaaag gcggtcataa aggccaccag cgccaccaca
    12121 aacatccacg atggaatgga aggcaccaga gcttcaaaat agattttcgc cagcagaatg
    12181 ttgatcatcg gcgcgaacag atagtcgagc agagaagacc aacccaccat aaagccgaca
    12241 gtcgggctaa tggatttctg ggcgtaagtg tatgcagagc cagcagaagg atagcggcga
    12301 accagcttcc cgtagctcag agccgtaaac aggatcgcaa tcaatgcgaa cgcataggct
    12361 gtcggcacat gaccatccgt gaggcctgaa acgataccaa atgtatcaaa cagcgtcatc
    12421 ggctgcatat aggctagacc catcattaca accggaacta acgtaagcgt tttacgcaat
    12481 tccacgcgag aggtgtttgg agtaacgtta tgcgacatgg tcattctcct ttacggtgat
    12541 aaccgtcgcg taagcaaaaa attgccccat ttttttggat tcctcagcga caacaactgt
    12601 cgatttttag taaatatcta tccggtacga agcccggcct cttggtatga atgattggtt
    12661 tgaagcaaaa aataaccgac gctaattaaa cgtcggtttt tagtcatttt ttgacagcgg
    12721 cgcattgtgc ctaaatgggg gggaaatgac aagagaatga gaggcttgtc ataataattt
    12781 ttctttaaat ggctgatttt ccgtcatcag attttgcata aacaccggcg atcgcactat
    12841 ttgctaaaat ttcgtcccgc caccaaccac tggcaagccc cgcggtctgt tcattccagc
    12901 caatccagac gggttcaaag agggtttggg cgaccacttt cttctcgatt aacgcaccac
    12961 tatcgagaaa acgttgcgcc agataacggg gtaaatagcc gcagccgagg ccgctaattt
    13021 gcagctccag cttggtttta aaatcgaaaa cggtaatagc ttcttgttcg tcgagaagtt
    13081 gtgaggccgt agaagcgccg gcctgtgcag tgtcccccac cacaatcgcc cgatatcgtt
    13141 taatgatacg acggtttaac ggttcttctt ccagggctaa cggatgatga ggcgcgaccg
    13201 caaagacttg ttccagatcg cccaaccgag agaaaccaaa ctcactggaa gatggtggct
    13261 catgcatcgc gccaacaata atatccgctc gcccctgagt cagggcatcc catgaaccac
    13321 cgagtacacc attgataaac ttcaggcgcg tcacgctatg atgttgataa aaagcctcaa
    13381 tgagtggcgc aagcagagaa aaagggaagg tgtcgtccac gccaatcacc agctcgtttt
    13441 cccagccttc atgcagtttg atcgcctgct tttccagttc tcgcacggta tgcagaactt
    13501 cccgcccttt ctctaataac atttttccgg tgcgggtgaa tttagcgcgg tgtccgctgc
    13561 gatcaagcaa ttgaatatta aggtcgcttt ccagcttgtg aacggtgtaa ctcagagcgg
    13621 acggcgtctt gtagagtttg gccgacgccg ccgcaaaact gccttctttt tctaaggcat
    13681 cgaggatcat caacacatcc agcagtggtt tcatacacgc ccccttgcag tgcatgcggc
    13741 gtcctgctgg cgtgatcgtt tactccagcg gcattaccag cggatcggga tactggtatt
    13801 caaaccccag ttcattacaa atccgactgc catcaataat cttacctttg ccgctgtcca
    13861 gactatttct gaactgcgga ggttccagcc ccagtaaacg ggccatctgc ggatagaaaa
    13921 cattacgcgc agggtgagct ggcgcacata tattatagat gtgtccgcct ttaggtgccc
    13981 gtaacaacag agtgatagcg ccaataacat cttctaaatg gactaaatta acaccatgtt
    14041 caccatcagg cgcggttttt ccggcaaaga agcgtccggg atgacgtccc ggtccgacca
    14101 ggcccgcaag acgcagaata tcgaccgaag taccgggtaa attgtgcagc cagtcttcga
    14161 gttcttctaa tactcgtcca ctgttggtca ccggattacg cggggtggtt tctttcaccg
    14221 tgccttgcgc gtcgccatag acagatgtcg agctggtaaa aataatgcgg ggaatacgat
    14281 gggccagcgc gctatccact aactcttgta ccgcttgtaa atagaactca tcgccggggc
    14341 cgctacgacg tgccggaagc gtaatgacca gcgcatcggc atccatcagg gcatccagat
    14401 cgtcagaatc gcaaactaac tcaggctcca tgcgaagcag atagctatca atgccactca
    14461 ttcgggccgc ttcgacacca tcttgtgtgg ttttactccc ggtgacttgc cagcctcgcg
    14521 ctgaaagtga catcgccagc ggcatgccta accaccctaa cccgacaatt gcgacctttt
    14581 tcatccgtta tctcctgata caccttagat ctataaggct acgctagcgt atcaaaactg
    14641 acaattcatt ctatgaatga atctgttcaa taatgataac gacatgctgc aatgagcagt
    14701 gaatcatcag taaccgcgta taccagacgg tgctcctctg taatgcgtcg ggaccagaaa
    14761 cctgacaaat tatgtttcag gggttctggt ttccccttac cttcaaatgg tgttctgcgg
    14821 gtatctttga taagttcatt gatcttttta acaattcgct tatctgtttc ctgccagtac
    14881 agataatcat cccatgattc ctcagaccag attagtttca ctcaataatg tccttttccg
    14941 ttcctttgcc tgatttcagg ctatcgattg agtccatcaa tctccgggcg ttagcggggg
    15001 aacgcagtag ataagccgtc tcttccagcg agttgtattc ttcgagtgac atcagaacac
    15061 aagcctctcc attctgacga gtaatgagga tcggggcatg atcttcaacg gctttcatca
    15121 ttgttgccga caaattctga cgcgcttcgc tgtagctaat tgtacgcatg taaatctcct
    15181 cttttgtaca gttcattgta caatgatggg tgttaattca ctatttatta attagtttgt
    15241 agatcaagac attgccagcg agacgaaaaa tcagactttg ctctttttgg tgctgtcaag
    15301 ttagaggaca gtcctcttag ccccctcctt tccccgctca ttcattaaac aaatccatcg
    15361 ccataaaata tataaaaaag cccttgcttt ctaacgtgaa agtggtttag gttagaagac
    15421 atcagttgaa taaacattca cagagacttt tatgacacgc gttcaattta aacaccacca
    15481 tcatcaccat catcctgact agtctttcag gcgatgtgtg ctggaagaca ttcagatctt
    15541 ccagtggtgc atgaacgcat gagaaagccc ccggaagatc accttccggg ggctttttta
    15601 ttgcgcggtt gttaacggtt cagacaggtt taaagaggaa taagaaaatg acagacaaca
    15661 ctcgtttacg catagctatg cagaaatccg gccgtttaag tgatgactca cgcgaattac
    15721 tggcgcgctg tggcattaaa atcaatcttc acacccagcg cctgatcgcg atggcagaaa
    15781 acatgccgat tgatattctg cgcgtgcgtg acgatgacat tcccggtctg gtaatggacg
    15841 gcgtggtaga ccttgggatt atcggcgaaa acgtgctgga agaagagctg cttaaccgcc
    15901 gcgcccaggg tgaagatccg cgctacttta ccttgcgtcg tctggatttc ggcggctgcc
    15961 gcctttcgct ggcaacgccg gttgatgaag cctgggacgg tccgctctcc ttaaatggta
    16021 aacgtatcgc cacctcttat cctcacctgc tcaagcgtta tctcgaccag aaaggtatct
    16081 cttttaaatc ctgcttactg aacggctctg tcgaagttgc gccgcgtgcc ggactggcgg
    16141 atgcgatttg cgatctggtt tccaccggtg ccacgctgga agctaacggc ctgcgcgaag
    16201 tcgaagttat ctaccgctcg aaagcctgcc tgatccagcg cgatggcgaa atggaagaat
    16261 ccaaacagca actgatcgac aaactgctga cccgtattca gggtgtgatc caggcgcgcg
    16321 aatcaaaata catcatgatg cacgcaccga ccgaacgtct ggatgaagtc atcgccctgc
    16381 tgccaggtgc cgaacgccca accattctgc cgctggcggg tgaccaacaa cgcgtagcga
    16441 tgcacatggt cagtagcgaa accctgttct gggaaacgat ggaaaaactg aaagcgctgg
    16501 gtgccagttc aatcctggtc ctgccgattg agaagatgat ggagtgatcg ccatgagctt
    16561 taacacaatc attgactgga atagctgtac tgcggagcaa caacgccagc tgttaatgcg
    16621 cccggcgatc tccgcttctg aaagcattac ccgcactgtt aacgatattc tcgataacgt
    16681 gaaagcacgc ggcaatgagg ccctgcggga atacagcgcg aagtttgata aaaccacggt
    16741 taccgcactg aaggtgtctg ctgaggaaat tgccgccgct agcgaacgcc tgagcgacga
    16801 gctaaaacag gagatggcgg tggcagtaaa gaatattgaa accttccaca ctgcgcaaaa
    16861 actgccgccg gtagatgtag aaacgcagcc aggcgtgcgt tgccagcagg tcacgcgccc
    16921 ggtcgcttca gttgggttgt atattcctgg cggctccgcc ccgctcttct caacggtatt
    16981 aatgctggca actccggcgc gtattgcggg ctgtaaaaaa gtggtgttgt gctcaccgcc
    17041 gccgattgcc gatgagatcc tttatgcggc gcagctgtgc ggtgtgcagg acgtgtttaa
    17101 cgtcggcggc gcacaggcca ttgccgcgct ggcgtttggt acggaatctg tgccgaaagt
    17161 ggacaaaatc ttcgggccgg gtaacgcctt tgtcaccgaa gcgaaacgtc aggtgagcca
    17221 gcgtctggac ggtgcggcga tcgatatgcc cgcaggcccg tcggaagtgc tggtgattgc
    17281 tgacagcggc gctacgccgg atttcgtggc ttctgatttg ctttctcagg ctgaacacgg
    17341 cccggactca caggtgattt tactgacgcc cgatgctgat atggcgcatc aagttgccga
    17401 ggccgtcgaa cgccaactgg cagaactgcc gcgtgccgaa accgcccgcc aggcactgag
    17461 cgccagccgc ctgatcgtga ctaaagattt agcgcagtgc gtagagattt ccaaccagta
    17521 cggcccggag cacctgatca ttcagacccg caacgcccgc gaactggtcg atggcatcac
    17581 cagcgccggt tcggtatttc ttggtgactg gtcaccggaa tcggcaggcg actatgcctc
    17641 cggcactaac catgttctac cgacttacgg ttacaccgcc acctgttcca gcctcgggct
    17701 ggcagatttc cagaagcgca tgaccgtgca ggaactctcg aaagaaggct tttccgcgct
    17761 ggcttcgacc attgaaacac tggcctccgc cgagcgcctg accgcccata aaaatgccgt
    17821 tactttgcgt gttaacgccc ttaaggagca agcatgagca ccgtgactat taccgattta
    17881 gcgcgtgaaa acgtccgcaa cctgacgccg tatcagtcgg cgcgtcgtct gggcggtaac
    17941 ggcgatgtct ggctgaacgc caacgaatac cccactgccg tggagtttca gcttactcag
    18001 caaacgctca accgctaccc ggaatgccag ccgaaagcgg tgattgaaaa ttacgcgcaa
    18061 tatgcaggcg taaaaccgga gcaggtgctg gtcagccgtg gcgcggacga aggtattgaa
    18121 ctactgattc gcgctttttg cgaaccgggt aaagacgcca tcctctactg cccgccaacg
    18181 tacggcatgt acagcgtcag cgccgaaacg attggcgtcg agtgccgcac agtgccgacg
    18241 ctggacaact ggcaactgga cttacagggc atttccgaca agctggacgg cgtaaaagtg
    18301 gtctatgttt gcagccccaa caacccgacc gggcaactga tcaacccgca ggattttcgc
    18361 accctgctgg agttaacgcg cggtaaggcg attgtggttg ccgatgaagc ctatatagag
    18421 ttttgtccac aggcatcgct ggctggctgg ctggcggaat atccgcacct ggctattttg
    18481 cgcacactgt cgaaagcttt tgctctggcg gggcttcgtt gcggatttac gctggcaaac
    18541 gaagaagtca tcaacctgct gatgaaagtg atcgccccct acccgctctc gacgccggtt
    18601 gccgacattg cggcccaggc gttaagcccg cagggaatta tcgccatgcg tgagcgggtg
    18661 gcgcaaatta ttgcagaacg cgaatacctg attgccgcac tgaaagagat cccctgcgtg
    18721 gagcaggttt ttgactctga aaccaactac attctggcgc gctttaaagc ctccagtgca
    18781 gtgtttaaat ctttgtggga tcagggcatt atcttacgtg atcagaataa acaaccctct
    18841 ttaagcggct gcctgcgaat taccgtcgga acccgtgaag aaagccagcg cgtcattgac
    18901 gccttacgtg cggagcaagt ttgatgagtc agaagtatct ttttatcgat cgcgatggaa
    18961 ccctgattag cgaaccgccg ggtgattttc aggtggaccg ttttgataaa ctcgcctttg
    19021 aaccgggcgt gatcccggaa ctgctgaagc tgcaaaaagc gggctacaag ctggtgatga
    19081 tcactaatca ggatggtctg ggaacacaaa gtttcccaca ggcggatttc gatggcccgc
    19141 acaacctgat gatgcagatc ttcacctcgc aaggcgtaca gtttgatgaa gtgctgattt
    19201 gtccgcacct gcccgccgat gagtgcgact gccgtaagcc gaaagtaaaa ctggtggaac
    19261 gttatctggc tgagcaagcg atggatcgcg ctaacagtta tgtgattggc gatcgcgcga
    19321 ccgacattca actggcggaa aacatgggca ttaatggttt acgctacgac cgcgaaatcc
    19381 tgaactggcc gatgattggc gagcaactca ctaaacgaga ccgttacgcc catgtagtgc
    19441 gcaacaccaa agagacgcaa attgacgtcc aggtgtggct ggatcgcgaa ggtggcagca
    19501 agattaacac cggtgttggc ttctttgatc acatgctgga tcagatcgcc acccacggcg
    19561 gtttccgtat ggaaatcaac gtcaaaggcg acctctatat cgacgatcac cacaccgtcg
    19621 aagataccgg cctggcgctg ggtgaagcgt taaaaattgc ccttggcgac aaacgcggta
    19681 tttgccgctt tggttttgtg ctaccgatgg acgaatgcct tgcccgctgc gcgctggata
    19741 tctctggtcg cccgcacctg gaatataaag ccgagtttac ctaccagcgc gtgggcgatc
    19801 tcagcaccga aatgatcgag cacttcttcc gttcgctctc atacaccatg ggcgtgaccc
    19861 tgcacctgaa aaccaaaggt aaaaacgatc accaccgtgt agagagcctg ttcaaagctt
    19921 ttggtcgcac cctgcgccag gccatccgcg tggaaggcga taccctgccc tcgtcgaaag
    19981 gagtgctgta atgaacgtgg tgatccttga tactggctgc gccaacctga actcggtgaa
    20041 gtctgccatt gcgcgccacg gttatgaacc caaagtcagc cgtgacccgg acgtagtgtt
    20101 gctggccgat aaactatttt tacccggcgt aggcaccgca caagcagcga tggatcaggt
    20161 gcgtgagcgc gaactgtttg atctcatcaa agcctgtacc caaccggtgc tgggcatctg
    20221 cttagggatg caactgcttg ggcggcgcag cgaagagagc aacggcgtcg atttgctggg
    20281 catcatcgac gaagacgtgc cgaaaatgac cgactttggt ctaccgctgc cccatatggg
    20341 ctggaaccgc gtttacccgc aggcaggcaa ccgcctgttt caggggattg aagacggcgc
    20401 gtacttttac tttgttcaca gctacgcaat gccggttaat ccgtggacca tcgcccagtg
    20461 taattacggc gaaccgttca ccgcggcggt acaaaaagat aacttctacg gcgtgcagtt
    20521 ccacccggag cgttctggtg ccgctggcgc taagttgctg aaaaacttcc tggagatgtg
    20581 atgattattc cggcattaga tttaattgac ggcactgtgg tgcgtctcca tcagggcgat
    20641 tacggcaaac aacgcgatta cggtaacgac ccgctgccgc gcttacagga ttacgccgcg
    20701 cagggtgccg aagtgttgca cctggtggat ctgaccgggg caaaagatcc ggcaaaacgt
    20761 caaatcccgc tgattaaaaa cctggtcgcg ggcgttaacg ttccggtaca ggttggcggc
    20821 ggcgtgcgta gcgaagatga cgtggcggcg ttactggaag cgggcgttgc gcgcgtggtg
    20881 gtcggctcca ccgcggtgaa ttcaccagaa agggtgaaag gctggtttga acgtttcggt
    20941 gccgatgcct tagtgctggc gctggatgtc cgtattgacg agcaaggcaa caagcaggtg
    21001 gcagtcagcg gctggcaaga gaactcgggc gtttcactgg aacaactggt ggaaacctat
    21061 ctgcccgtcg gcctgaaaca tgtgctgtgt accgatatct cgcgcgacgg cacgctggca
    21121 ggttctaacg tctctttata tgaagaagtg tgcgccagat atccgcaggt ggcatttcag
    21181 tcatccggcg gcattggcga cattaacgat gttgctgccc tgcgtggcac tggcgtgcgc
    21241 ggcgtaatag ttggtcgggc attactggaa ggtaaattca ccgtgaagga ggccatcgca
    21301 tgctggcaaa acgcataatt ccgtgtctcg acgttcgtga tggtcaggtg gtgaaaggcg
    21361 tacagtttcg caaccatgaa atcattggcg atatcgtgcc gctggcaaaa cgctacgcag
    21421 aggaaggtgc agacgaactg gtgttctacg atatcaccgc ttccagcgat ggccgtgtgg
    21481 tagataaaag ctgggtatct cgcgtggcgg aagtgatcga cattccgttt tgtgtggcgg
    21541 gtgggattaa gtctctggat gacgccgcca aaattctttc ctttggcgcg gataaaattt
    21601 ccatcaactc tcctgcgctg gcggatccga cgttaattac tcgcctggcg gatcgctttg
    21661 gcgtgcagtg tattgtggtc ggcattgata cctggtacga cgctgaaacc ggtaaatatc
    21721 atgtgaatca atataccggc gatgaaaccc gcacccgcgt cactcagtgg gaaacgctcg
    21781 actgggtaca ggaagtgcaa aaacgcggtg ccggagaaat cgtcctcaac atgatgaatc
    21841 aggacggcgt gcgtaacggt tacgacctcg aacaactgaa aaaagtgcgt gaagtttgcc
    21901 acgtcccgct gattgcctct ggtggcgcgg gtaccatgga acacttcctc gaagccttcc
    21961 gcgatgccga cgttgacggc gcgctggcag cttccgtatt ccacaaacaa ataatcaata
    22021 ttggtgattt aaaagcgtac ctggcaacac agggcgtgga gatcaggata tgttaacaga
    22081 acaacaacgt cgcgaactgg actgggaaaa aaccgacgga cttatgccgg tgattgtgca
    22141 acacgcggta tctggcgaag tgctaatgct gggctatatg aacccggaag ccttagacaa
    22201 aaccatcgaa agcggcaaag tcaccttctt ctcgcgcact aaacagcgac tgtggaccaa
    22261 aggcgaaacg tcgggcaatt tcctcaatgt ggtgagtatt gcgccggact gcgacaacga
    22321 cacgttactg gtgctggcga atcccatcgg gccaacctgc cacaaaggca ccagcagctg
    22381 cttcggcgac accgctcacc agtggctgtt cctgtatcaa ctggaacaac tgctcgccga
    22441 acgtaaatct gccgacccgg aaacctccta caccgccaaa ctgtatgcca gcggcaccaa
    22501 acgcattgcg cagaaagtgg gcgaagaagg cgtggaaacc gcactggccg caacggtaaa
    22561 tgaccgcttt gagctgaaga atgaggcatc tgacttggtg tatcacctac tggtgttgtt
    22621 gcaggatcag gatctggatt taacaacggt gattgagaac ctgcgtaaac ggcatcagtg
    22681 agttgcgtgc tgaccggatg cgatgttgtt gtcgcatccg gcaaaaaaac gggcaaggtg
    22741 tcaccaccct gcccgttttc tttaaaaccg aaaagattac ttcacgctgt aattacgcag
    22801 agcgttacgc cccagcataa tccctgcacc caccataccg cccagcagta ctgccagaat
    22861 caatgtaatt gcctttttcg ggctatcgcg acgaataggt aacgtcggtt tcattacata
    22921 gcggtaagca tgaatatcaa gatcatcaac ctttaagttt tcaatatcca acaggttttg
    22981 acgtgtctga tagtagtttg gtgagaacac caacggacga gtcgcttgat gcttaatcat
    23041 cgactccagc gcttcgctcc cccagtctgc tgaacctgtg gctttgtcac ctgcgcctga
    23101 ttcgcatact gcaacgcttc ctgaatctga cggatatgca gatctttctg ttcttgagcg
    23161 acaacttcct gcgtcttcag cgaatcctgc aagtttttca ttcgcagcgc gatgttgtct
    23221 ttcaggtctt gttccagtac ttcatttacc tggtcgtcaa cttgctggat atacttcgcc
    23281 agctgtttct gcgcaccttc aggtgtttca ccaacataag aaactgctaa aggtaatgac
    23341 tgatttttga cggtaggctc aatggtcaac ttttctgctt cttcctgatt atccagcgtt
    23401 tctgctaatg cagaaaatgt cgtgctgaaa cctccgcaac cccatgtttt cacataactg
    23461 ttgcgttgac caattgaatc tacagtagcc ttttttaata tttcattctc catttcaatg
    23521 cgttgtagct ttttcctcag ctcacgtatt tcgatttgtt ctggtgttat cggagaggct
    23581 tttggggttt tgccctgacg ctcatcacac agttgtttga cccatcttgt cattgtggaa
    23641 aggccaacat ccatagcttt ggcggcatct gccaccgtgt agttctggtc aacaaccagt
    23701 tgagcggatt cgcgtttaaa ctctgcgctg aaatttcttt ttttcattgg agcacctgtg
    23761 ttgttctgag gtgagcatat cacctctgtt caggtggcca aattcagtgt gccacttcac
    23821 catgaatgct ataggataaa tgataaaaat aatgtattat cgcaaagcaa atataagaca
    23881 atcagggcga aaatcgccct gataacagga tgttagtcac agccaaagag atcgcgggtg
    23941 tagactttgt ctgccacatc cttaagctct tctgccatac ggttggagat aatgacgtcg
    24001 gcctgttgtt tgaaagtggc gaaatcacgt tccaggcgag agttgaagaa ggagtcttct
    24061 ttcatcaccg gctcgtaaat gatcacttca acgcctttcg ccttgatacg cttcataatc
    24121 ccctgaatgg aagacgcgcg gaagttatct gacccgctct tcataatcag acgataaata
    24181 cccaccactt gcggtttgcg tgacaaaatg gcatcggcaa taaaatcttt tcgcgtgcgg
    24241 tttgcatcga caattgccga aatcaggtta ttcggcacag actggtagtt agccagtaac
    24301 tgcttggtat ctttcggcag acaataacca ccgtagccaa acgacgggtt gttgtaatgg
    24361 ttaccaatac gcggatcgag acaaacacct tcgataatct ggcgagtatt cagacctaaa
    24421 ctttccgcat aactatccag ctcgttaaag tacgccacgc gcatcgccag ataggtgttt
    24481 gcaaaaagtt taatcgcttc tgcttcagtg gagtcggtaa acagtgtagg gatattttgc
    24541 ttaatcgctc cttcctgtaa taacgcagca aaacgttctg cgcgctccga atgctcaccg
    24601 atgacaatac gtgacggatg aaggttatcg taaagggctt taccttcacg gagaaattcc
    24661 ggagaaaaaa taaggttatc gatacccaga cgttctttaa ttaatttggt gaaacctacc
    24721 ggaattgtag atttaatcac cattaccgca ttgggattaa tttctgtcac atcgcgaatg
    24781 accgcttcca cgcttgaggt attaaaataa tttgttttcg ggtcataatc ggtaggcgtg
    24841 gcaataataa cgtaatcggc atttttatac gcgtcatact tatctgtcgt agcgcggaaa
    24901 ttgagatctt tagtcgccag atactcttca atctccttat caacaatcgg tgactgcttc
    24961 ttgttaagaa tgtccacttt ggcctgaacg atatccagtg caaccacttc gtgattttgc
    25021 gcaatcagaa taccatttga aagaccaaca taacctgttc ctgaaattgt tattttcatt
    25081 agctctgact tcttccggtt aaacatttag tgtgatcatt aattcaccac tacattttca
    25141 tctactgcgg gatttttaac gctgtagtag atgaactgtc aaggcagcga tcctgctgtg
    25201 cggctctgta ttatatcgct tttttaaccc caaattataa aaaaaggcca taacctaccg
    25261 ctttgtataa aaaaagcccg gagggtttct ccgggccttg cattgattaa ttgattaaaa
    25321 tcagattaat ccagccattc ggtatggaac acaccttctt tatcaatgcg cttataagta
    25381 tgcgcaccga aatagtcacg ctgtgcctgg attaggttcg caggcagaac tgcggcgcgg
    25441 tagctgtcgt aataggcaac cgcagcagcg aaggtaggaa cagggatacc gttctgtact
    25501 gcataagcga cgacatagcg cagcgcctgc tggtagtcat cggcaatttg cttgaagtac
    25561 ggagccagca gcaggttagc gatctgcgga ttttcggcat atgcatcagt gattttctgc
    25621 aggaactgcg cacggataat gcagccagca cggaaaatct tcgcgatttc accgtagttc
    25681 agatcccagt tgtactcttc agacgcagca cgcagctgag agaaaccctg ggcgtaagaa
    25741 acgattttgc ccagatacag agcacgacga actttttcga tgaactcagc cttgtcgcca
    25801 gctggctgcg cttgcgggcc agagagaact ttagatgcgg caacgcgctg atctttcaga
    25861 gaagagatgt atcgtgcaaa cacagactcg gtaatcagcg acagcggttc gccgagatcc
    25921 agtgcgctct ggctggtcca tttaccggta cctttgttag ccgcttcatc aaggatcaca
    25981 tcaaccaagt agttaccgtc ttcatctttt ttggtgaaga tatctttggt gatgtcgatc
    26041 aggtagctgc tcagttcacc gttattccac tcggtaaagg tctgcgccag ttcttcgttg
    26101 gtgaggttca agccaccttt aagcagggaa taggcttcag caatcagctg catatctcca
    26161 tattcaatac cgttgtgaac catcttcaca tagtgacctg cgccatcagc accgatatag
    26221 gtaacacatg gctccccgtc ttcagccact gcggcgattt tagtcaggat cggcgcaacc
    26281 agttcatagg cttctttctg cccaccaggc ataatggaag gacctttcag cgcgccctct
    26341 tcaccgccgg aaacaccggt accaatgaag tgaaagcctt cggcagaaag ctcacggtta
    26401 cgacgaatag tgtccaagaa gaaggtgtta ccaccatcaa tgatgatgtc acctttatcg
    26461 agatatggtt tgagggaatc aattgcagca tccgtacctg cacctgcttt caccattaaa
    26521 aggatgcgac gaggcgtttc cagagattca acaaactctt tcaccgtata gtaaggaact
    26581 agattcttgc ctggatttcc ctcgatcact tcttccgtct tttcacgaga acgattaaaa
    26641 atagagacag tgtaaccacg actttcaatg ttgagcgcaa ggttgctccc catcacagcc
    26701 ataccgtaaa caccaatatg ttgttgtgac atttattctc ctattttaaa catagttacc
    26761 ctattcatag aatagtataa cgtaaaaaat caaaatccaa tcacatacag taaaggtgta
    26821 gctaatgagc caatagttac tcagaatccc catagaaaga ttatatttgg cttttctaac
    26881 ccgttat
//
LOCUS       DAJOWB010000059        26466 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJOWB010000059 DAJOWB010000000
VERSION     DAJOWB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.059"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            47..1516
                     /gene="gspA"
                     /locus_tag="OHO85_002635"
     CDS             47..1516
                     /gene="gspA"
                     /locus_tag="OHO85_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HCQ2671991.1"
                     /translation="MSTRREVILSWLCEKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSIGRLTEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIALTCGWLLLSSFTATLPVPAWLIPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTHKWFESVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1518..1937
                     /gene="gspB"
                     /locus_tag="OHO85_002636"
     CDS             1518..1937
                     /gene="gspB"
                     /locus_tag="OHO85_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HCQ2671992.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWVLWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPGVA
                     VENAPSSSEDEENTVEESDEKAGLRERVKNALNELER"
     gene            2175..2486
                     /gene="rpsJ"
                     /locus_tag="OHO85_002637"
     CDS             2175..2486
                     /gene="rpsJ"
                     /locus_tag="OHO85_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCQ2671993.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2519..3148
                     /gene="rplC"
                     /locus_tag="OHO85_002638"
     CDS             2519..3148
                     /gene="rplC"
                     /locus_tag="OHO85_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCQ2671994.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3159..3764
                     /gene="rplD"
                     /locus_tag="OHO85_002639"
     CDS             3159..3764
                     /gene="rplD"
                     /locus_tag="OHO85_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCQ2671995.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3761..4063
                     /gene="rplW"
                     /locus_tag="OHO85_002640"
     CDS             3761..4063
                     /gene="rplW"
                     /locus_tag="OHO85_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCQ2671996.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4081..4902
                     /gene="rplB"
                     /locus_tag="OHO85_002641"
     CDS             4081..4902
                     /gene="rplB"
                     /locus_tag="OHO85_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCQ2671997.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4919..5197
                     /gene="rpsS"
                     /locus_tag="OHO85_002642"
     CDS             4919..5197
                     /gene="rpsS"
                     /locus_tag="OHO85_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCQ2671998.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5212..5544
                     /gene="rplV"
                     /locus_tag="OHO85_002643"
     CDS             5212..5544
                     /gene="rplV"
                     /locus_tag="OHO85_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCQ2671999.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5562..6263
                     /gene="rpsC"
                     /locus_tag="OHO85_002644"
     CDS             5562..6263
                     /gene="rpsC"
                     /locus_tag="OHO85_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCQ2672000.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6276..6686
                     /gene="rplP"
                     /locus_tag="OHO85_002645"
     CDS             6276..6686
                     /gene="rplP"
                     /locus_tag="OHO85_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCQ2672001.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6686..6877
                     /gene="rpmC"
                     /locus_tag="OHO85_002646"
     CDS             6686..6877
                     /gene="rpmC"
                     /locus_tag="OHO85_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCQ2672002.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6877..7131
                     /gene="rpsQ"
                     /locus_tag="OHO85_002647"
     CDS             6877..7131
                     /gene="rpsQ"
                     /locus_tag="OHO85_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCQ2672003.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7296..7667
                     /gene="rplN"
                     /locus_tag="OHO85_002648"
     CDS             7296..7667
                     /gene="rplN"
                     /locus_tag="OHO85_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCQ2672004.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7678..7992
                     /gene="rplX"
                     /locus_tag="OHO85_002649"
     CDS             7678..7992
                     /gene="rplX"
                     /locus_tag="OHO85_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCQ2672005.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8007..8546
                     /gene="rplE"
                     /locus_tag="OHO85_002650"
     CDS             8007..8546
                     /gene="rplE"
                     /locus_tag="OHO85_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCQ2672006.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8561..8866
                     /gene="rpsN"
                     /locus_tag="OHO85_002651"
     CDS             8561..8866
                     /gene="rpsN"
                     /locus_tag="OHO85_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCQ2672007.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8900..9292
                     /gene="rpsH"
                     /locus_tag="OHO85_002652"
     CDS             8900..9292
                     /gene="rpsH"
                     /locus_tag="OHO85_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCQ2672008.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9305..9838
                     /gene="rplF"
                     /locus_tag="OHO85_002653"
     CDS             9305..9838
                     /gene="rplF"
                     /locus_tag="OHO85_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCQ2672009.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9848..10201
                     /gene="rplR"
                     /locus_tag="OHO85_002654"
     CDS             9848..10201
                     /gene="rplR"
                     /locus_tag="OHO85_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCQ2672010.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10216..10719
                     /gene="rpsE"
                     /locus_tag="OHO85_002655"
     CDS             10216..10719
                     /gene="rpsE"
                     /locus_tag="OHO85_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCQ2672011.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10723..10902
                     /gene="rpmD"
                     /locus_tag="OHO85_002656"
     CDS             10723..10902
                     /gene="rpmD"
                     /locus_tag="OHO85_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCQ2672012.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10906..11340
                     /gene="rplO"
                     /locus_tag="OHO85_002657"
     CDS             10906..11340
                     /gene="rplO"
                     /locus_tag="OHO85_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCQ2672013.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11348..12679
                     /gene="secY"
                     /locus_tag="OHO85_002658"
     CDS             11348..12679
                     /gene="secY"
                     /locus_tag="OHO85_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCQ2672014.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12711..12827
                     /gene="rpmJ"
                     /locus_tag="OHO85_002659"
     CDS             12711..12827
                     /gene="rpmJ"
                     /locus_tag="OHO85_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCQ2672015.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12974..13330
                     /gene="rpsM"
                     /locus_tag="OHO85_002660"
     CDS             12974..13330
                     /gene="rpsM"
                     /locus_tag="OHO85_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCQ2672016.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13347..13736
                     /gene="rpsK"
                     /locus_tag="OHO85_002661"
     CDS             13347..13736
                     /gene="rpsK"
                     /locus_tag="OHO85_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCQ2672017.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13770..14390
                     /gene="rpsD"
                     /locus_tag="OHO85_002662"
     CDS             13770..14390
                     /gene="rpsD"
                     /locus_tag="OHO85_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCQ2672018.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14416..15405
                     /gene="rpoA"
                     /locus_tag="OHO85_002663"
     CDS             14416..15405
                     /gene="rpoA"
                     /locus_tag="OHO85_002663"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCQ2672019.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15446..15829
                     /gene="rplQ"
                     /locus_tag="OHO85_002664"
     CDS             15446..15829
                     /gene="rplQ"
                     /locus_tag="OHO85_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCQ2672020.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15936..16304
                     /locus_tag="OHO85_002665"
     CDS             15936..16304
                     /locus_tag="OHO85_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCQ2672021.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16315..16740
                     /gene="zntR"
                     /locus_tag="OHO85_002666"
     CDS             16315..16740
                     /gene="zntR"
                     /locus_tag="OHO85_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCQ2672022.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16796..17014
                     /gene="arfA"
                     /locus_tag="OHO85_002667"
     CDS             16796..17014
                     /gene="arfA"
                     /locus_tag="OHO85_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCQ2672023.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(17011..17421)
                     /gene="mscL"
                     /locus_tag="OHO85_002668"
     CDS             complement(17011..17421)
                     /gene="mscL"
                     /locus_tag="OHO85_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCQ2672024.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17551..18927)
                     /gene="trkA"
                     /locus_tag="OHO85_002669"
     CDS             complement(17551..18927)
                     /gene="trkA"
                     /locus_tag="OHO85_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCQ2672025.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18949..20238)
                     /gene="rsmB"
                     /locus_tag="OHO85_002670"
     CDS             complement(18949..20238)
                     /gene="rsmB"
                     /locus_tag="OHO85_002670"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCQ2672026.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGTEEGDGFFYAKLIKK"
     gene            complement(20284..21231)
                     /gene="fmt"
                     /locus_tag="OHO85_002671"
     CDS             complement(20284..21231)
                     /gene="fmt"
                     /locus_tag="OHO85_002671"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCQ2672027.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21246..21755)
                     /gene="def"
                     /locus_tag="OHO85_002672"
     CDS             complement(21246..21755)
                     /gene="def"
                     /locus_tag="OHO85_002672"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCQ2672028.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21885..23009
                     /gene="dprA"
                     /locus_tag="OHO85_002673"
     CDS             21885..23009
                     /gene="dprA"
                     /locus_tag="OHO85_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCQ2672029.1"
                     /translation="MVDTDIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22981..23454
                     /locus_tag="OHO85_002674"
     CDS             22981..23454
                     /locus_tag="OHO85_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCQ2672030.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23483..24025
                     /locus_tag="OHO85_002675"
     CDS             23483..24025
                     /locus_tag="OHO85_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCQ2672031.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCLACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            24030..24602
                     /gene="tsaC"
                     /locus_tag="OHO85_002676"
     CDS             24030..24602
                     /gene="tsaC"
                     /locus_tag="OHO85_002676"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCQ2672032.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24607..25425
                     /gene="aroE"
                     /locus_tag="OHO85_002677"
     CDS             24607..25425
                     /gene="aroE"
                     /locus_tag="OHO85_002677"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCQ2672033.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALSMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKCNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25422..25679
                     /locus_tag="OHO85_002678"
     CDS             25422..25679
                     /locus_tag="OHO85_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCQ2672034.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGSVWLP"
     gene            complement(25655..26209)
                     /locus_tag="OHO85_002679"
     CDS             complement(25655..26209)
                     /locus_tag="OHO85_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCQ2672035.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTEP"
BASE COUNT         6749 a         6252 c         6923 g         6542 t
ORIGIN      
        1 ttatttttat gattaaggat aatcaataat taaaacagga agttctatgt ctacgagaag
       61 agaagttatt ctttcctggt tgtgtgagaa acgtcaaacc tggcgtctat gctatttgtt
      121 gggtgaggct ggaagtggaa aaacctggct ggcgcagcaa ctgcaaaaag ataaacatcg
      181 ccgtgtgatt actttaagcc tcgttgtttc ctggcaaggt aaggccgcat ggatcgttac
      241 cgacgataac gcggctgaac agggctgccg tgacagcgcc tggacgcgag atgagatggc
      301 ggggcaatta ctgcatgcgc ttcatcgaac tgatagtcgc tgccctctta ttattattga
      361 aaatgctcac ctgaatcacc gccgcatact tgatgatctc caacgcgcaa tatctcttat
      421 tcctgacggt caatttttgc tgatcggcag acccgatcga aaagtcgaac gtgattttaa
      481 aaaacagggc attgaacttg tctcgatagg acgtctgacg gagcacgaac ttaaggcaag
      541 catccttgaa ggtcagaata ttgaccaacc cgatctcctt ttaaccgcca gagttctgaa
      601 acggatagct ttattatgtc ggggcgatcg cagaaagctg gcgcttgccg gcgaaacaat
      661 tcgcttatta cagcaagccg agcagaccag cgttttcaca gcaaaacaat ggcgaatgat
      721 ttaccgcata ttgggtgata accgcccccg gaaaatgcag cttgccgtgg tgatgagtgg
      781 aaccattatc gctcttacct gtggttggct actgctgtcc tcttttacag ctacgttacc
      841 cgttcctgcg tggctgatac ccgtaacgcc agtagttaaa caagatatga ctaaggatat
      901 tgctcatgtg gtgatgcgcg atagtgaggc gctaagcgtg ttatacggcg tatgggggta
      961 cgaggtccca gctgattcgg cctggtgcga tcaggcagta agagcggggc tggcatgtaa
     1021 aagtgggaat gcttctttac aaacattggt agatcagaat ttgccctgga tcgcatcgct
     1081 taaagtcggg gacaaaaaat tgcctgttgt ggtcgttcgc gtcggtgagg ctagcgttga
     1141 tgtcctcgta ggtcagcaaa cgtggacgct gacacataaa tggtttgagt cggtatggac
     1201 gggtgattat cttctgttgt ggaaaatgtc gccggaaggg gagagtacca ttacgcgtga
     1261 tagcagcgag gaagagattc tctggctgga aacgatgtta aaccgcgcat tgcatatttc
     1321 gacagaacct tcggcggagt ggcgtccctt gttggtagaa aaaataaagc aatttcaaaa
     1381 aagccaccac ttgaaaactg acggtgttgt tggcttttca acgttagtac atctctggca
     1441 ggtggcaggg gagagtgcct acttatatcg ggatgaagca aatatttccc cggaaacaac
     1501 agtgaagggg aaatgacatg tttgaattct atatcgccgc gcgtgagcag aaagaaacag
     1561 gtcaccctgg tatcttctcc cgacaaaaac acagcaccat aatatatgta atttgtttat
     1621 tactgatttg cctgtggttt gcaggaatgg tgctggttgg tgggtatgcc aggcagctat
     1681 gggtgctttg gatagtaaaa gccgaagtca ctgtagaggc tgaaacgccc gcttttaaac
     1741 aatcaacaca acactatttc ttcaaaaaac agccactccc cgtcgtcgaa tccgtcgagg
     1801 aggaagatga cccaggcgtc gcggttgaga atgccccctc ttcttctgaa gacgaagaaa
     1861 atactgtgga agaaagcgac gagaaagccg gtttaagaga gcgtgtcaaa aatgcactga
     1921 acgaacttga gagataaccc gaaggctgtt tactttacta gcaatacgct tgcgttcggt
     1981 ggttaagtat gtataatgcg cgggcttgtc gtagttgaca gcaggttcaa tctgaacctc
     2041 agttagccga atttggctac ctaacaatgc tcccaatcgg ggagctacgt aagaacggtt
     2101 acactctccc atcaatcgta atgggtctga ggagtaatca ttttcgttta taaaataatt
     2161 ggagctctgg tctcatgcag aaccaaagaa tccgtatccg cctgaaagcg tttgatcatc
     2221 gtctgatcga tcaagcaacc gcggaaatcg tcgagactgc caagcgcact ggtgcgcagg
     2281 tccgtggtcc gatcccgctg ccgacacgca aagagcgctt cactgttctg atctccccgc
     2341 acgtcaacaa agacgcgcgc gatcagtacg aaatccgtac tcacttgcgt ctggttgaca
     2401 tcgttgagcc aaccgagaaa accgttgatg ctctgatgcg tctggatctg gctgccggtg
     2461 tagacgtgca gatcagcctg ggttaatcag gtcattgagc gattgagagg ttgaaacaat
     2521 gattggttta gtcggtaaaa aagtgggtat gacccgtatc ttcacagaag acggcgtttc
     2581 tatcccagta accgtaatcg aagttgaagc aaaccgcgtt actcaggtta aagacctggc
     2641 taacgatggc taccgtgcta ttcaggtgac caccggtgct aaaaaagcta accgtgtgac
     2701 caagcctgaa gctggccact tcgctaaagc tggcgtagaa gctggccgtg gtctgtggga
     2761 attccgcctg gctgaaggcg aagagttcac tgtaggtcag agcattagcg ttgaactgtt
     2821 tgctgacgtt aaaaaagttg acgtaactgg cacctctaaa ggtaaaggtt tcgcaggtac
     2881 cgttaagcgc tggaacttcc gtacccagga cgctactcac ggtaactcct tgtctcaccg
     2941 cgttccgggt tctatcggtc agaaccagac tccgggcaaa gtgttcaaag gcaagaaaat
     3001 ggcaggtcag atgggtaacg aacgtgtaac cgttcagagc cttgacgtag tacgcgttga
     3061 cgctgagcgc aacctgctgc tggttaaagg tgctgtcccg ggtgcaaccg gtagcgacct
     3121 gatcgttaaa ccagctgtga aggcgtaagg agatagcaat ggaattagta ttgaaagacg
     3181 cgcagagcgc gctgactgtt tccgaaacta ccttcggtcg tgatttcaac gaagcgctgg
     3241 ttcaccaggt tgttgttgct tatgcagctg gtgctcgtca gggtactcgt gctcagaaga
     3301 ctcgtgctga agtaactggt tccggtaaaa aaccgtggcg ccagaaaggc accggccgtg
     3361 cgcgttctgg ttctatcaag agcccgatct ggcgttctgg tggcgtgacc tttgctgctc
     3421 gtccgcagga ccacagtcaa aaagttaaca agaagatgta ccgcggcgcg ctgaaaagca
     3481 tcctgtccga actggtacgt caggatcgtc tgatcgttgt cgagaagttc tctgtagaag
     3541 cgccgaaaac taagctgctg gcacagaaac tgaaagacat ggctctggaa gatgtgctga
     3601 tcatcaccgg tgagctggac gaaaacctgt tcctggctgc gcgcaacctg cacaaggttg
     3661 acgtacgcga tgcaactggt atcgacccgg ttagcctgat cgccttcgac aaagtcgtaa
     3721 tgactgctga tgctgttaag caagttgagg agatgctggc atgattcgtg aagaacgtct
     3781 gctgaaggtg ctgcgtgcac cgcacgtttc tgaaaaagcg tctactgcga tggaaaaatc
     3841 caacaccatc gtactcaaag ttgctaaaga cgcgaccaaa gcagaaatca aagctgctgt
     3901 gcagaaactg tttgaagtcg aagtcgaagt cgttaacacc ctggtagtta aagggaaagt
     3961 taaacgtcac ggacagcgta tcggtcgtcg tagcgactgg aaaaaagctt acgtcaccct
     4021 gaaagaaggc cagaatctgg acttcgttgg cggcgctgag taagtcggag gagtaataca
     4081 atggcagttg ttaaatgtaa accgacatct ccgggtcgtc gccacgtagt taaagtggtt
     4141 aaccctgagc tgcacaaggg caaacctttt gctccgttgc tggaaaaaaa cagcaaatcc
     4201 ggtggtcgta acaacaatgg ccgtatcacc actcgtcata tcggtggtgg ccacaagcag
     4261 gcttaccgta ttgttgactt caaacgcaac aaagacggta tcccggcagt tgttgaacgt
     4321 cttgagtacg atccgaaccg ttccgcgaac atcgcgctgg ttctgtacaa agacggtgaa
     4381 cgccgttaca tcctggcccc taaaggcctg aaagctggcg accagattca gtctggcgtt
     4441 gatgctgcaa tcaaaccagg taacaccctg ccgatgcgca acatcccggt tggttctact
     4501 gttcataacg tagaaatgaa accaggtaaa ggcggtcagc tggcacgttc cgctggtact
     4561 tacgttcaga tcgttgctcg tgatggtgct tatgtcaccc tgcgtctgcg ttctggtgaa
     4621 atgcgtaaag tagaagcaga ctgccgtgca actctgggcg aagttggcaa tgctgagcat
     4681 atgctgcgcg ttctgggtaa agcaggtgct gcacgctggc gtggtgttcg tccgaccgtt
     4741 cgcggtaccg cgatgaaccc ggtagaccac ccacatggtg gtggtgaagg tcgtaacttt
     4801 ggtaagcacc cggtaactcc gtggggcgtt cagaccaaag gtaagaagac ccgcagcaac
     4861 aagcgtactg ataaattcat cgtacgtcgc cgtagcaaat aattttagag gataagccat
     4921 gccacgttct ctcaagaaag gtccttttat tgacctgcac ttgctgaaga aggtagagaa
     4981 agcggtggaa agcggagaca agaagcccct gcgcacttgg tcccgtcgtt caacgatctt
     5041 tcctaacatg atcggtttga ccatcgctgt ccataatggt cgtcagcacg ttccggtatt
     5101 tgtaaccgac gaaatggtcg gtcacaaact gggtgaattc gcaccgactc gtacttatcg
     5161 cggccacgct gctgataaaa aagcgaagaa gaaataaggt aggaggaaga gatggaaact
     5221 atcgctaaac atcgccatgc tcgttcttct gctcagaagg ttcgccttgt tgctgacctg
     5281 attcgcggta agaaagtgtc gcaggctctg gatattttga cctacaccaa caagaaagcg
     5341 gctgtactgg tcaagaaagt tctggaatct gccattgcta acgctgaaca caacgatggc
     5401 gctgacattg acgatctgaa agttacgaaa attttcgtag acgaaggccc gagcatgaag
     5461 cgcattatgc cgcgtgcaaa aggtcgtgca gatcgcatcc tgaagcgcac cagccacatc
     5521 actgtggttg tgtccgatcg ctgagactct ggagactagc aatgggtcag aaagtacatc
     5581 ctaatggtat tcgcctgggt attgtaaaac catggaactc tacctggttt gcgaacacca
     5641 aagaattcgc tgacaacctg gacagcgatt ttaaagtacg tcagtacctg actaaggaac
     5701 tggctaaagc gtccgtatct cgtatcgtta tcgagcgtcc ggctaagagc atccgtgtaa
     5761 ccattcacac tgctcgcccg ggtatcgtta tcggtaaaaa aggtgaagac gtagaaaaac
     5821 tgcgtaaggt cgtagcggac atcgctggcg ttcctgcaca gatcaacatc gccgaagttc
     5881 gtaagcctga actggacgca aaactggttg ctgacagcat cacttctcag ctggaacgtc
     5941 gcgttatgtt ccgtcgtgct atgaagcgtg ctgtacagaa cgcaatgcgt ctgggcgcta
     6001 aaggtattaa agttgaagtt agcggccgtc tgggcggcgc ggaaatcgca cgtaccgaat
     6061 ggtaccgcga aggtcgcgta ccgctgcaca ctctgcgtgc tgacatcgac tacaacacct
     6121 ctgaagcgca caccacttac ggtgtaatcg gcgttaaagt gtggatcttc aaaggcgaga
     6181 tcctgggtgg tatggctgct gttgaacaac cggaaaaacc ggctgctcag cctaaaaagc
     6241 agcagcgtaa aggccgtaaa taaggagcgt cgctgatgtt acaaccaaag cgtacaaaat
     6301 tccgtaaaat gcacaaaggc cgtaaccgcg gtctggcgca gggtacggat gttagcttcg
     6361 gcagcttcgg tctgaaagct gttggccgtg gtcgtctgac tgcccgtcag atcgaagcag
     6421 cacgtcgtgc tatgacccgt gcagttaagc gtcaaggtaa gatctggatc cgtgtgttcc
     6481 cggacaaacc gatcactgaa aagccgctgg cagtgcgtat gggtaaaggt aaaggtaacg
     6541 tggagtattg ggttgccttg attcagccgg gtaaagtcct gtatgaaatg gacggtgttc
     6601 cggaagagct ggcccgtgaa gcattcaagc tggcagcagc gaaactgccg attaaaacca
     6661 cctttgtaac taagacggtg atgtaatgaa agcaaaagag ctgcgtgaga agagcgttga
     6721 agagctgaac accgagctgc tgaacctgct gcgtgagcag ttcaacctgc gtatgcaggc
     6781 tgcaagtggc cagctgcaac agtctcacct gttgaagcaa gtgcgtcgcg atgtcgcacg
     6841 cgttaagact ttactgaacg agaaggcggg tgcgtaatga ccgataaaat ccgtactctg
     6901 caaggtcgcg ttgttagcga caaaatggag aaatccattg ttgttgctat cgaacgtttt
     6961 gtgaaacacc cgatctacgg taaattcatc aagcgtacga ccaaactgca cgtacatgac
     7021 gagaacaacg aatgcggtat cggtgacgtg gttgaaatcc gcgaatgccg tccgctgtcc
     7081 aagactaaat cctggacgct ggttcgcgtt gtagagaaag cggttctgta atacagtaca
     7141 ctctctcgat acgaataaac ggctcagaaa tgagccgttt attttttcta cccatatcct
     7201 tgaagcggtg ttataatgcc gcgccctcga tatggggatt tttaacgacc tgattttcgg
     7261 gtctcagtag tagttgacat tagcggagca ctaaaatgat ccaagaacag actatgctga
     7321 acgtcgccga caactccggt gcacgtcgcg taatgtgtat caaggttctg ggtggctcgc
     7381 accgtcgcta cgcaggcgta ggcgacatca tcaagatcac catcaaagaa gcaattccgc
     7441 gtggtaaggt caaaaaaggt gatgtgctga aggcggtagt ggtgcgcacc aagaagggtg
     7501 ttcgtcgccc ggacggttct gtcattcgct tcgatggtaa tgcttgtgtt cttctgaaca
     7561 acaacagcga gcagcctatc ggtacgcgta tttttgggcc ggtaactcgt gagcttcgta
     7621 gtgagaagtt catgaaaatt atctctctgg caccagaagt actctaagga gcgaatcatg
     7681 gcagcgaaaa tccgtcgtga tgacgaagtt atcgtgttaa ccggtaaaga taaaggtaaa
     7741 cgcggtaaag ttaagaatgt cctgtcttcc ggcaaggtca ttgttgaagg tatcaacctg
     7801 gttaagaaac atcagaagcc ggttccggcc ctgaaccaac cgggtggcat cgttgaaaaa
     7861 gaagccgcta ttcaggtttc caacgtagca atcttcaatg cggcaaccgg caaggctgac
     7921 cgtgtaggct ttagattcga agacggcaaa aaagtccgtt tcttcaagtc taacagcgaa
     7981 actatcaagt aatttggagt agtacgatgg cgaaactgca tgattactac aaagacgaag
     8041 tagttaaaaa actcatgact gagtttaact acaattctgt catgcaagtc cctcgggtcg
     8101 agaagatcac cctgaacatg ggtgttggtg aagcgatcgc tgacaaaaaa ctgctggata
     8161 acgcagcagc agacctggca gcaatctccg gtcaaaaacc gctgatcacc aaagcacgca
     8221 aatctgttgc aggcttcaaa atccgtcagg gctatccgat cggctgtaaa gtaactctgc
     8281 gtggcgaacg catgtgggag ttctttgagc gcctgatcac tattgctgta cctcgtatcc
     8341 gtgacttccg tggcctgtcc gctaagtctt tcgacggtcg tggtaactac agcatgggtg
     8401 tccgtgagca gatcatcttc ccagaaatcg actacgataa agtcgaccgc gttcgtggtt
     8461 tggatattac cattaccact actgcgaaat ctgacgaaga aggccgcgct ctgctggctg
     8521 cctttgactt cccgttccgc aagtaaggta gggttactaa atggctaagc aatcaatgaa
     8581 agcacgcgaa gtaaaacgcg tagctttagc tgataaatac ttcgcgaaac gcgctgaact
     8641 gaaagcgatc atctctgatg tgaacgcttc cgacgaagat cgttggaacg ctgttctcaa
     8701 gctgcagact ctgccgcgtg attccagccc gtctcgtcag cgtaaccgct gccgtcaaac
     8761 aggtcgtccg catggtttcc tgcggaagtt cgggttgagc cgtattaagg tccgtgaagc
     8821 cgctatgcgc ggtgaaatcc cgggtctgaa aaaggctagc tggtaattgt caccaattga
     8881 atcacgggag taaagacaga tgagcatgca agatccgatc gcggatatgc tgacccgtat
     8941 ccgtaacggt caggccgcga acaaagctgc ggtcaccatg ccttcctcca agctgaaagt
     9001 ggcaatcgcc aacgtgctga aggaagaagg ttttattgaa gattttaaag ttgaaggcga
     9061 caccaagcct gaactggaac ttactctgaa gtatttccag ggcaaagctg ttgtagaaag
     9121 cattcagcgt gtcagccgcc caggtctgcg catctataaa cgtaaagatg agctgccgaa
     9181 agttatggcg ggtctgggta tcgcagttgt ttctacctct aaaggtgtta tgactgatcg
     9241 tgcagcgcgc caggctggtc ttggtggcga aattatctgc tacgtagcct aatcggagga
     9301 aaaaatgtct cgtgttgcta aagcaccggt cgttgttcct gccggcgttg acgtaaaaat
     9361 caacggtcag gttattacga tcaaaggtaa aaacggcgag ctgactcgta ctctcaacga
     9421 tgctgttgaa gttaaacatg cagataatac cctgaccttc ggtccgcgtg atggttacgc
     9481 agacggttgg gcacaggctg gtaccgcgcg tgccctgctg aactcaatgg ttatcggtgt
     9541 taccgaaggc ttcactaaga agctgcagct ggttggtgta ggttaccgtg cagcggttaa
     9601 aggcaatgtg attaacctgt ctctgggttt ctctcatcct gttgaccatc agctgcctgc
     9661 gggtatcact gctgaatgtc cgactcagac tgaaatcgtg ctgaaaggcg ctgataagca
     9721 ggtgatcggc caggttgcag cggatctgcg cgcctaccgt cgtcctgagc cttataaagg
     9781 caagggtgtt cgttacgccg acgaagtcgt gcgtaccaaa gaggctaaga agaagtaagg
     9841 taacactatg gataagaaat ctgctcgtat ccgtcgtgcg acccgcgcac gccgcaagct
     9901 ccaggagctg ggcgcaactc gcctggtggt acatcgtacc ccgcgtcaca tttacgcaca
     9961 ggtaattgca ccgaacggtt ctgaagttct ggtagctgct tctactgtag aaaaagctat
    10021 cgctgaacaa ctgaagtaca ccggtaacaa agacgcggct gcagctgtgg gtaaagctgt
    10081 cgctgaacgc gctctggaaa aaggcatcaa agatgtatcc tttgaccgtt ccgggttcca
    10141 atatcatggt cgtgtccagg cactggcaga tgctgcccgt gaagctggcc ttcagttcta
    10201 aggtagaggt gtaagatggc tcacatcgaa aaacaagctg gcgaactgca ggaaaagctg
    10261 atcgcggtaa accgcgtatc taaaaccgtt aaaggtggtc gtattttctc cttcacagct
    10321 ctgactgtag ttggcgatgg taacggtcgc gttggttttg gttacggtaa agcgcgtgaa
    10381 gttccagcag cgatccagaa agcgatggaa aaagcccgtc gcaatatgat taacgtcgcg
    10441 ctgaataacg gcactctgca acaccctgtt aaaggtgttc acacgggttc tcgcgtattc
    10501 atgcagccgg cttccgaagg taccggtatc atcgccggtg gtgcaatgcg cgccgttctg
    10561 gaagtcgctg gggttcataa cgttctggct aaagcatatg gttccaccaa cccgatcaac
    10621 gtggttcgtg caactattga tggcctggaa aatatgaatt ctccagaaat ggtcgctgcc
    10681 aagcgtggta aatccgttga agaaattctg gggaaataaa ccatggcaaa gactattaaa
    10741 attactcaaa cccgcagtgc aatcggtcgt ctgccgaaac acaaggcaac gctgcttggc
    10801 ctgggtctgc gtcgtattgg tcacaccgta gagcgcgagg atactcctgc tattcgcggt
    10861 atgatcaacg cggtttcctt catggttaaa gttgaggagt aagagatgcg tttaaatact
    10921 ctgtctccgg ccgaaggctc caaaaaggcg ggtaaacgcc tgggtcgtgg tatcggttct
    10981 ggcctcggta aaaccggtgg tcgtggtcac aaaggtcaga aatctcgttc tggcggtggc
    11041 gtacgtcgcg gtttcgaggg tggtcagatg cctctgtacc gtcgtctgcc gaaattcggc
    11101 ttcacttctc gtaaagcagc gattacagcc gaagttcgtc tgtctgacct ggctaaagta
    11161 gaaggcggtg ttgtagacct gaacacgctg aaagcggcta acattatcgg tatccagatc
    11221 gagttcgcga aagtgatcct ggctggcgaa gtaacgactc cggtaactgt tcgtggcctg
    11281 cgtgttacta aaggcgctcg tgctgctatc gaagctgctg gcggtaaaat cgaggaataa
    11341 gtagcagatg gctaaacaac cgggattaga ttttcaaagt gccaaaggtg gcttaggcga
    11401 gctgaaacgc agactgctgt ttgttatcgg tgcgctgatt gtgttccgta ttggctcttt
    11461 tattccgatc cctggtattg atgccgctgt acttgccaaa ctgcttgagc aacagcgagg
    11521 caccatcatt gagatgttta acatgttctc tggtggtgct ctcagccgtg cttctatctt
    11581 tgctctgggg atcatgccgt atatttcggc gtcgatcatt atccagctgc tgacggtggt
    11641 tcacccaacg ttggcagaaa ttaagaaaga aggggagtct ggtcgtcgta agatcagcca
    11701 gtacacccgt tacggtactc tggtgctggc aatattccag tcgatcggta ttgctaccgg
    11761 tctgccgaat atgcctggta tgcaaggcct ggtgattaac ccaggctttg cattctactt
    11821 caccgctgtt gtaagtctgg tcacaggaac gatgttcctg atgtggttgg gcgaacagat
    11881 tactgaacga ggtatcggca acggtatttc aatcattatc ttcgccggta ttgtcgcggg
    11941 actcccgcca gccattgccc atactatcga gcaagcgcgt caaggcgacc tgcacttcct
    12001 cgtgttgctg ttggttgcag tattagtatt tgcagtgacg ttctttgttg tatttgttga
    12061 gcgtggtcaa cgccgcattg tggtaaacta cgcgaaacgt cagcaaggtc gtcgtgtcta
    12121 tgctgcacag agcacacatt taccgctgaa agtgaatatg gcgggggtaa tcccggcaat
    12181 cttcgcttcc agtattattc tgttcccggc gaccatcgcg tcatggttcg ggggcggtac
    12241 tggttggaac tggctgacaa caatttcgct gtatttgcag cctgggcaac cgctttatgt
    12301 gttactctat gcgtctgcaa tcatcttctt ctgtttcttc tacacggcgt tggttttcaa
    12361 cccgcgtgaa acagcagata acctgaagaa gtccggtgca tttgtaccag gaattcgtcc
    12421 gggagagcaa acggcgaagt atatcgataa agtaatgacc cgcctgaccc tggttggtgc
    12481 gctgtatatt acctttatct gcctgatccc ggagttcatg cgtgatgcaa tgaaagtacc
    12541 gttctacttc ggtgggacct cactgcttat cgttgttgtc gtgattatgg actttatggc
    12601 tcaagtgcaa actctgatga tgtccagtca gtatgagtct gcattgaaga aggcgaacct
    12661 gaaaggctac ggccgataat tggtcgcccg agaagttacg gagagtaaaa atgaaagttc
    12721 gtgcttccgt caagaaatta tgccgtaact gcaaaatcgt taagcgtgat ggtgtcatcc
    12781 gtgtgatttg cagtgccgag ccgaagcata aacagcgcca aggctgattt tttcgcatat
    12841 ttttcttgca aagttgggtt gagctggcta gattagccag ccaatctttt gtatgtctgt
    12901 gcgtttccat ttgagtatcc tgaaaacggg cttttcagca tggaacgtac atattaaata
    12961 gtaggagtgc atagtggccc gtatagcagg cattaacatt cctgatcata agcatgccgt
    13021 aatcgcatta acttcgattt atggcgtcgg caagacccgt tctaaagcca tcttggctgc
    13081 agcgggtatc gctgaagatg ttaagatcag tgagctgtct gaaggacaaa tcgacacgct
    13141 gcgtgacgaa gttgccaaat ttgtcgttga aggtgatctg cgccgtgaaa tcagcatgag
    13201 catcaagcgc ctgatggatc ttggttgcta tcgcggtttg cgtcatcgtc gtggtctccc
    13261 ggttcgcggt cagcgtacca agaccaacgc acgtacccgt aagggtccgc gcaaaccgat
    13321 caagaaataa tcggggtgat tgaataatgg caaaggcacc aattcgtgca cgtaaacgtg
    13381 taagaaaaca agtctctgac ggcgtggctc atatccatgc ttctttcaac aacaccatcg
    13441 tgactatcac tgatcgtcag ggtaacgcgt tgggttgggc aacagccggt ggttccggtt
    13501 tccgtggttc tcgcaaatcc actccgtttg cagctcaggt tgcagcagag cgttgcgctg
    13561 acgccgtgaa agaatacggc atcaagaatc tggaagttat ggttaaaggt ccgggtccag
    13621 gccgcgaatc tactattcgt gctctgaacg ccgcaggttt ccgcatcact aacattactg
    13681 atgtgactcc gatccctcat aacggttgtc gtccgccgaa aaaacgtcgc gtataacgcc
    13741 tcgttttcca ggtttgttgg agaaagaaaa tggcaagata tttgggtcct aagctcaagc
    13801 tgagccgtcg tgagggcacc gacttattcc ttaagtctgg cgttcgcgcg atcgatacca
    13861 agtgtaaaat tgaacaagct cctggccagc acggtgcgcg taaaccgcgt ctgtctgact
    13921 atggtgtgca gttgcgtgaa aagcaaaaag ttcgccgtat ctatggtgtg ctggagcgtc
    13981 agttccgtaa ctactacaaa gaagcagcac gtctgaaagg caacaccggt gaaaacctgt
    14041 tggctctgct ggaaggtcgt ctggacaacg ttgtataccg tatgggcttc ggtgccactc
    14101 gtgcagaagc acgtcagctg gttagccata aagcaattat ggtaaacggt cgtgttgtta
    14161 acatcgcttc ttatcaggtt agtccgaatg acgttgtaag cattcgtgag aaagcgaaga
    14221 agcagtctcg cgtgaaagcc gctctggagc tggctgagca gcgtgaaaag ccaacctggc
    14281 tggaagttga tgctggcaag atggaaggta cgtttaagcg taagccggag cgttctgatc
    14341 tgtctgcgga cattaacgaa cacctgatcg tcgagcttta ctccaagtaa agcttagtac
    14401 caaagagagg acacaatgca gggttctgtg acagagtttc taaaaccgcg cctggttgat
    14461 atcgagcaag tgagttcgac gcacgccaag gtgacccttg agcctttaga gcgtggcttt
    14521 ggccatactc tgggtaacgc actgcgccgt attctgctct catcgatgcc gggttgcgcg
    14581 gtgaccgagg ttgagattga tggtgtacta catgagtaca gcaccaaaga aggcgttcag
    14641 gaagatatcc tggaaatcct gctcaacctg aaagggctgg cggtgagagt tcagggcaaa
    14701 gatgaagtta ttcttacctt gaataaatct ggcattggcc ctgtgactgc agccgatatc
    14761 acccacgacg gtgatgtcga aatcgtcaag ccgcagcacg tgatctgcca cctgaccgat
    14821 gagaacgcgt ctattagcat gcgtatcaaa gttcagcgcg gtcgtggtta tgtgccggct
    14881 tctacccgaa ttcattcgga agaagatgag cgcccaatcg gccgtctgct ggtcgacgca
    14941 tgctacagcc ctgtggagcg tattgcctac aatgttgaag cagcgcgtgt agaacagcgt
    15001 accgacctgg acaagctggt catcgaaatg gaaaccaacg gcacaatcga tcctgaagag
    15061 gcgattcgtc gtgcggcaac cattctggct gaacaactgg aagctttcgt tgacttacgt
    15121 gatgtacgtc agcctgaagt gaaagaagag aaaccagagt tcgatccgat cctgctgcgc
    15181 cctgttgacg atctggaatt gactgtccgc tctgctaact gccttaaagc agaagctatc
    15241 cactatatcg gtgatctggt acagcgtacc gaggttgagc tccttaaaac gcctaacctt
    15301 ggtaaaaaat ctcttactga gattaaagac gtgctggctt cccgtggact gtctctgggc
    15361 atgcgcctgg aaaactggcc accggcaagc atcgctgacg agtaaccgga tcacaggtta
    15421 aggttttact gagaaggata aggtcatgcg ccatcgtaag agtggtcgtc aactgaaccg
    15481 caacagcagc catcgccagg ctatgttccg caatatggca ggttcactgg ttcgtcatga
    15541 aatcatcaag acgactctgc ctaaagcgaa agagctgcgc cgcgtagttg agccgctgat
    15601 tactcttgcc aagactgata gcgttgctaa tcgtcgtctg gcattcgccc gtactcgtga
    15661 taacgagatc gtggcaaaac tgtttaacga actgggcccg cgtttcgcga gccgtgccgg
    15721 tggttacact cgtattctga agtgtggctt ccgtgcaggc gacaacgcgc cgatggctta
    15781 catcgagctg gttgatcgtt cagagaaagc agaagctgct gcagagtaat ctgaagcaac
    15841 gtaaaaaaac ccgccccggc gggttttttt atacccgtag catccccact tatctacaat
    15901 agctgtactc tttttgttca tcccctggag tatttatgtg gttacttgac cagtgggcag
    15961 agcgtcatat agcagaagcg caagcgaaag gtgagtttga taacctggca ggtagcggcg
    16021 aaccattgat actggatgat gattctcacg tgccaccgga attacgtgcg gggtatcgct
    16081 tgctgaagaa tgccggttgc ttaccgccag aacttgagca acggagagaa gcaattcagc
    16141 ttctggatat cctgagaggt atccgtcacg atgatccgca atatcaagag gttagccgtc
    16201 gattgtcatt actggaattg aagctgcgac aagctggatt gagtaccgat tttttacgcg
    16261 gcgattatgc tgacaagttg ttggacaaaa tcaacgataa ctagtggagt atgtatgtat
    16321 cgcattggtg agctggcaaa aatggcggaa gtaacacctg acacgattcg ttattacgaa
    16381 aaacagcaga tgatggagca tgaagtgcgt actgaaggcg ggtttcgcct gtataccgaa
    16441 agcgatctcc agcgattgaa atttatccgc catgccagac aactaggttt cagtctggag
    16501 tcgatccgcg agttgctgtc gatccgcatc gatcctgaac accatacctg tcaggagtca
    16561 aaaggcattg tgcaggaaag attgcaggaa gtcgaagcac ggatagccga gttgcagagt
    16621 atgcagcgtt ccttgcaacg ccttaacgat gcctgttgtg ggaccgccca tagcagtgtt
    16681 tattgttcga ttcttgaagc tcttgaacaa ggggcgagtg gcgttaagag tggctgttga
    16741 ttttttgcac tggcaggatt acactcgcgc cgtaaaataa ccaattggag tttttatgag
    16801 ccgatatcag catactaaag ggcagataaa ggataatgcg atagaagcat tactacatga
    16861 tcccttattc cgacagcgcg tagagaaaaa taagaagggg aaaggcagtt acatgcgaaa
    16921 aggaaaacat ggcaatcggg ggaactggga ggccagtggc aagaaagtaa atcacttttt
    16981 taccactggc cttctgcttt caggcgtttg ttaagagcgg ttattctgct ctttcagcaa
    17041 atcacgaatt tctgtcagta atacttcttc tttagttggt gctggtgcgg ctgctggttc
    17101 ttcttttttc cgattcagtt tgttgattag cttaatcgcc ataaagatgg caaaggccac
    17161 aatcagaaaa tcaaagacgt tttgaatgaa gacaccgtaa tgcatcacaa cagcagggat
    17221 atccccctgc gcatcgcgta gcgtgacagc aaactgttta aagtcgatcc cgccaattaa
    17281 taagcccaga ggtggcatga tgatatcggc aaccagtgaa gatacaatct tcccgaatgc
    17341 cgcaccgata atgacaccca ccgccaaatc caccacgttc ccgcgcatcg caaattcgcg
    17401 aaattcttta ataatgctca tgttattctc cctatgaagc cgacaaccat aagtctaaca
    17461 aatgttaagc cattttcctg ccaggaatga atttaattaa tccttgataa tcaaagggaa
    17521 ataataaggc gtcattagac gccttattaa ttacaagaag aaagggcttg gctggaagag
    17581 tctttcgacg tcggtaataa actttttatc tgtgaggaac ataattacgt gatcgccttg
    17641 ctcaatgcgc agattgtcat tggcaatcat cacgtcgttt ccacgtacca ccgctccaat
    17701 aatcgttcct ggcggtagct tgatttcgtc aatgactctg ccgacaacgc gtgaggtgct
    17761 ttcatcaccg tgagcaacgg cttcaatagc ttctgctacg ccgcggcgca atgaggaaac
    17821 accaacaata tctgcttttc gcacatggct aagcaacgca gaaatagttg cttgttgtgg
    17881 tgaaatcgca atatcgatca cgctcccctg aaccagatcc acataagcgc gacgctggat
    17941 caataccatc acctttttcg cacccatacg tttggcaagc atggcggaca tgatattggc
    18001 ctcgtcatcg ttggtgacgg caataaacag atcaacttga tcgatatgtt cttcggccag
    18061 cagttcttga tccgacgcat caccaaaaaa gacgatcgta ttctgcaact tttccgccag
    18121 ttcggcagcg cgctgctgat tacgttcgat gagtttaacg ctgtaatctt tttccagacg
    18181 acgcgccagc cctgcaccga tattaccgcc accaaccagc atgatccgct tatacggttt
    18241 ttccagtcgc tgtaattcac tcatcaccgc acggatatgc tgtgaagcgg caataaagaa
    18301 cacttcatca ccagcttcca caatggtcga accttgtgga cgaatggggc gatcgtggcg
    18361 gaaaattgct gccacacgag tatcgatatg tggcatatgt tcgcgcatgg tcgaaagtgc
    18421 gttaccaatc agcgggccgc cataataggc tttaaccaca gcgaggctga ctttaccctc
    18481 agcgaagttc accacctgca atgcgccggg atactcaatc agtcggtaaa tattatcgat
    18541 aaccaactgc tctggtgcga tcagatgatc aatcggtaca gcatctgaat gaaatagctt
    18601 atcggcatcg cgcacgtagt ctggtgagcg gatgcgagcg atgcgattag gggtgttgaa
    18661 aagtgagtag gctacctggc aggcaaccat attggtttca tctgaactgg ttacagcaac
    18721 cagcatatcg gcgtcgtcgg cacctgcctc ccgcaatacg cgtggatgag agccatgccc
    18781 ctgcacgact cgcaggtcaa atttatcctg caaggtccgc agacgctcac cgttggtatc
    18841 gacaacagtg atatcgttgt tctcgccaac caggttttcc gccagtgtgc cgccaacctg
    18901 gccggcaccc agaatgataa ttttcatcag tcgcgacccg ttatctcatc actttttgat
    18961 tagcttagcg taaaagaagc cgtcgccctc ttcggtacca ggcagatttt gtttacccgg
    19021 ttgctctggt gttcctgttt cgcaaagttc ggcatcagcg gtacgttgca aaaaggcttt
    19081 aatctgcagg ctattctctt ccggtaacac cgaacaggtg gcatagacca gggttccacc
    19141 ggattttaaa tgcggccaaa tggcgtcgag aatttcagac tgcaattgcg cgagttccgg
    19201 gatatcgcga tcgcgacgta accatttaat atccggatgg cgacgaatca caccggttgc
    19261 tgaacaaggc gcatctaata aaatgcgatc aaactgttgc tcgccacacc attgggaagg
    19321 gtagcggcca tcaccttgtt tcacggtcgc tttcatacca aggcgtttta aattgtcgta
    19381 aacgcgagag aggcgctgtt cgtcaatatc aaccgcaaca acctgcgctt ctggtgccac
    19441 ctcaaggata tgcgttgttt taccgccggg ggcggcacaa agatccagaa tgtgttcacc
    19501 gttttgtggc gcaagccagg tcatgcaacc ttgtgctgat gcatcctgaa cggtaaccca
    19561 tccgtcttca aaaccaggta gcgcatgaac aggtgcaggt gtttccagcc gtacagcatc
    19621 agggtaatcc gcatgcggga aacctttcat tcctgcttca tccaacaatg caagccagct
    19681 gtcgcgggaa tgatgcgtac gattaacgcg cagccacatt ggcggacgct ggttattggc
    19741 ttcgacgatg gattgccact gctctggata cgctttttgc agacgcttca gcaaccagga
    19801 aggatgcaga taacgtgcat cactggcatt aaactcggct aataactctt cttgctgacg
    19861 ctggaactgg cgtaatacac cgtttatcaa ccctttaagt tgcggacgct taattgcgat
    19921 agcgccttca accgtttcag ccagcgcagc atgaggtgga atgcgggtat aaagcagttg
    19981 atacaaacca accataatca ggtaatgcac ggtccgctgt ttgccggtca tcggacgggc
    20041 cattaactta ttaatcagcc agtctaactg cgaaagcgta cgcagtacgc caaagcacaa
    20101 ctcttgaaga agtgctttgt ctttatcgga aactttttgc tggagcggtg gcagaatgtt
    20161 gcttaatgat tgcccttgct cgacgacttg ttcaacggcc tgggccgcca tgctacgtaa
    20221 attacgttgt tttttcataa gtataaaaat gcccggcaag accgggctta gaagagtgga
    20281 ctatcagacc agacggttgc ccggaacaaa ccattcccga cgagagttca ggaggtcttg
    20341 cgcgctcatc gctttcttac ccgcaggttg taacgagagc aggttcagga tgccatcacc
    20401 agtcgcaacc tgaatgcctt gtttattggc ttcaaggatc gttcctggtg cagcgttggt
    20461 tgccgtatca atgaccgacg ctttccagac tttaaccggt tgtccttcaa tttccagcca
    20521 gctcattggc catgggttga aagcgcgaat gcagcgttca agctgcgctg ccgaaagcga
    20581 ccagtcaata cgcgcttctt ctttactcaa cttctcggcg taagtgacaa gagtttcgtc
    20641 ctgaacttct ggtttcgccg tgccgtctgc cagttgtttc aacgtggtga taagcccttg
    20701 tgggccaagc tctgccagct tgtcgtacag cgtaccactg gtatcttctg cagtaatcgg
    20761 gcaggagagc ttatagagca tatcaccggt gtctaaaccg acatccattt gcataatggt
    20821 cacaccagtt tctgcatcac ccgcccatag tgagcgttgg attggtgcag caccgcgcca
    20881 gcgtggcagc agtgaaccat gaacgttgat acagccaaga cgcggcatct ccagcactgc
    20941 tttaggcaga attaagccat aggcgacgac gaccataaca tcagcctgca gatcggcgac
    21001 cagttgctgg ttttcttgtg gacgcaggga aacaggttga aaaacgggca gacctttttc
    21061 ctcagccaga actttaaccg ggctgggcat cagtttttta ccgcgtcctg ccggtcggtc
    21121 tggctgggtg aacacgccaa cgacgttatg accagaagac aacagcgcgt cgagatgacg
    21181 cgctgcaaag tcaggtgtac ccgcaaaaat aatacgtagt gattctgaca cgttagttct
    21241 tatccttaag cccgggcttt cagacgatcc agtttttcaa ctttctgacg aatacgttgt
    21301 tgtttcaacg gtgacagata atccataaac agtttgccga ccaggtgatc catctcatgc
    21361 tgaatacaga tggctaacag accgtctgct tccagttcaa atggtttacc gtcgcggtcc
    21421 agggcgcgaa ttttaacttt ctctgcgcgt ggcactaaag cacgttgttc agggatcgac
    21481 aggcaacctt cttcaatgcc tgtttcgccg cttttttcta aaagctccgg attgattaac
    21541 accagccgtt cgtcacggtt ttccgaaaca tcaataacaa tgatacgttg atggatatca
    21601 acctgggttg ccgccaggcc aataccttct tctgcgtaca tcgtctcgaa catatcatcg
    21661 acgatacgct gaatttctgc attcacttct tctaccggtt tagcaacttt gcgaagccgc
    21721 tcgtccggaa tatgtaacac ttgcaaaact gacataaatc tccagagatg tgttcaggag
    21781 ttagaaagat tatttcttct attctagaca aatccccctc tgattgacag catcactgac
    21841 caatcgcaaa gattgctaag gctgcttatg gcagggagat aaggatggtc gatacagata
    21901 tttggctgcg tttaatgagc atcagcagct tgtacggcga tgatatggtc cgtatagctc
    21961 actggctggc aaaacagtcg catattgatg cggttgtatt gcagcaaaca gggcttacat
    22021 tgcggcaggc acaacgcttt ctttcatttc cgcgaaagag tatcgaaagc tcactttgtt
    22081 ggttggagca acccaaccat catttaatcc ctgcggacag cgaattttat cctcctcaac
    22141 ttctggtgac gacagattac cccggcgcac tgtttgttga aggagaactg cacgcgctgc
    22201 attcatttca gcttgccgta gtggggagtc gggcgcattc atggtatggc gagcgatggg
    22261 gacggttatt ttgcgaaact ctggcgacgc gtggagtgac aattacgagt ggactggcgc
    22321 gtggaatcga tggtgtggcg cataaagctg ccttacaggt aaatggcgtc agcattgctg
    22381 tattggggaa tggacttaat accattcatc cccgccgcca tgcccgactg gctgccagtc
    22441 tgcttgaaca tggcggagct ctcgtctcgg aatttcccct cgatgttcca ccccttgctt
    22501 acaatttccc acgaagaaat cgcattatca gtggtctaag taaaggtgta ctggtggtgg
    22561 aagcggcttt gcgcagtggt tcgctggtga cagcacgttg tgcgcttgag caggggcgtg
    22621 aagtttttgc cttgccaggt ccaataggga atccgggaag cgaagggcct cactggttaa
    22681 taaaacaagg tgcgattctt gtgacggaac cggaagaaat tctggaaaac ttgcaatttg
    22741 gattgcactg gttgccagac gcccctgaaa attcatttta ttcaccagat cagcaagacg
    22801 tggcattgcc atttcctgag ctcctggcta acgtaggaga tgaggtaaca cctgttgacg
    22861 tcgtcgctga acgtgccggc caacctgtgc cagaggtagt tactcaacta ctcgaactgg
    22921 agttagcagg atggatcgca gctgtacccg gcggctatgt ccgattgagg agggcatgcc
    22981 atgttcgacg tactaatgta tttgtttgaa acctatattc acacagaagc tgagttgcgt
    23041 gtggatcaag acaaacttga acaggatctt accgacgcag gatttgatcg agaagatatc
    23101 tacaatgccc tgctttggct ggaaaagctt gcggattatc aggaaggact ggcagaaccg
    23161 atgcaactgg cctctgatcc actctccatg cgtatttata cccctgagga gtgtgaaaga
    23221 ctggatgcca gctgtcgtgg ttttctgctt ttccttgagc aaattcaggt gcttaacctt
    23281 gaaacccgtg aaatggtgat agagcgagtg ctcgcgctgg ataccgcaga gttcgacctg
    23341 gaagacctga aatgggtaat cctgatggtg ttgttcaata ttccgggctg cgaaaatgcg
    23401 taccagcaaa tggaagaatt actctttgaa gtgaatgaag gtatgctgca ttaatcattt
    23461 ctttaattca gcataagttg ttatggcgaa atcagcactg ttcacggtgc gtaataatga
    23521 gtcctgccca aagtgcgggg ctgaactggt tattcgatcc gggaaacacg gtccgtttct
    23581 tggatgctca cagtatccgg cgtgtgacta cgtccgtcct ctgaaatctt cagcggatgg
    23641 acatattgtc aaagttctgg aggggcaggt ttgccttgca tgtggcgcaa atctggtatt
    23701 acgccaggga cgctttggta tgtttattgg ttgcagtaac taccctgaat gcgaacatac
    23761 cgaacttatc gataaaccgg acgaaacagc aattacatgc ccccaatgcc ggacgggcca
    23821 tctggtccag cgccgctccc gttatggcaa aacatttcac tcctgtgatc gctacccgga
    23881 gtgtcaattt gccattaact tcaaacccat agctggagaa tgccctgagt gtcattatcc
    23941 actactcatc gaaaagaaaa ccgcgcaggg tgtaaaacac ttttgtgcca gtaaacaatg
    24001 tggaaagccg gtttcggcgg aataataacg tgaataataa cctgcaagga gacgctatcg
    24061 cagctgcgat agatgtcctc aatgaagaac gtgtcatcgc ctatccaacg gaagccgttt
    24121 tcggtgttgg gtgcgatcct gatagcgaaa cagcagtgat gagactgttg gagttaaaac
    24181 agcgtccggt tgataagggg ctgattttaa tcgcggcaaa ttacgagcag cttaaaccct
    24241 atattgatga caccatgctg actgatgcgc agcgtgaaac cattttttcc cgctggccag
    24301 gtcctgtcac ctttgtcttt cccgcgcctg cgacaacacc gcgctggttg acgggccgct
    24361 ttgattcgct tgctgtacga gtcaccgacc atccgttggt ggttgctttg tgccaggctt
    24421 atggtaaacc gctggtttct accagtgcca acttgagtgg attgccacct tgtcgaacag
    24481 tagacgaagt tcgcgcacaa tttggcgcgg cgttcccggt tgtgcctggt gaaacggggg
    24541 ggcgtttaaa tccttcagaa atccgcgatg ccctgacggg tgaactgttt cgacaggggt
    24601 aacataatgg aaacctatgc tgtttttggt aatccgatag cccacagcaa atcgccattc
    24661 attcatcagc aatttgctca acaactgaat attgaacatc cctatgggcg cgtgttggca
    24721 cccatcaatg atttcatcaa cacactgaac gctttcttta gtgctggtgg taaaggtgcg
    24781 aatgtgacgg taccttttaa agaagaggct tttgccagag cggatgagct tactgaacgg
    24841 gcagcgttgg ctggtgctgt taataccctc aagcggttag aagatggacg cttgcagggt
    24901 gacaataccg atggtatagg tttgttaagc gatctggaac gtctgtcttt tatccgccct
    24961 ggtttacgta ttctgcttat cggcgctggt ggagcatctc gcggcgtact actgccactt
    25021 ctttccctgg actgtgcggt gacaatcact aatcggacgg tatcccgcgc ggaagagttg
    25081 gctaaattgt ttgcgcacac tggtagtatt caggcgttga gtatggacga actggaagga
    25141 catgagtttg atctcattat taatgcaaca tccagtggca tcagtggtga tattccggcg
    25201 atcccgtcat cgctcattca tccaggcatt tattgctatg acatgttcta tcagaaagga
    25261 aaaactcctt ttctggcatg gtgtgagcag cgaggctcaa agtgtaatgc tgatggttta
    25321 ggaatgctgg tggcacaggc ggctcatgcc tttcttctct ggcacggtgt tctgcctgac
    25381 gtagaaccag ttataaagca attgcaggag gaattgtccg cgtgaatcag gccatccagt
    25441 ttccggacag agaagagtgg gacgagaata aaaaatgtgt atgttttccc gctctcgtga
    25501 atggcatgca actgacatgt gcgatctctg gcgagagtct ggcgtatcgt tttactggag
    25561 atacgccaga acagtggtta gcgagttttc gtcagcatcg ctgggatctg gaagaagaag
    25621 cggaaaactt aattcaggaa caaagtgaag atgatcaagg ctcggtctgg ttaccctgat
    25681 ccagatattc gtccttccat ttcacgtaat tattcgcgga atagcgtaac ccagccttct
    25741 cttcatcact taacgggcgg atctgtttga cggggctacc gagatacaga tatccgctct
    25801 ccagccgttt attttgtggg accagactac ccgcaccaat catcacatca tcttctacta
    25861 ttgcgccatc aagtaaaatt gagcccatcc caaccaaaac tcgattgcca atggtgcagc
    25921 cgtggagcat caccttgtga ccaacagtga catcttcgcc aatggttaat gggttgccat
    25981 ctgggttgta cgaggattta tgagtgacat gcaacatact gccatcctgg atattggtgc
    26041 gtgctccgat ctgtacataa tgtacatctc cacgaatcac aacgagcggc cagatcccca
    26101 catcatcagc cagacgaacg tcaccaatca cgacactgct atcgtcgatc attacgcgct
    26161 gaccgatttg tggaaaaaga tcgcggtatg ggcgtaaaac atcagacata cttacctcag
    26221 caataaatga tttactaatg actttggggg cattattggc cttgtgcaag tcttttagta
    26281 tgcaaaaaag caccgttttg tgtgcgattg cagcaaaaag ggtgaaaaaa caacaaacag
    26341 aaaaaaagat caaaaaaata cttgtgcaaa aaattgggat ccctataatg cgcctccgtt
    26401 gagacgacaa cgtgaaacac ttcacaagat ggtcgtaaca acgaagagaa aaaatcctga
    26461 aattca
//
LOCUS       DAJOWB010000060        25811 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJOWB010000060 DAJOWB010000000
VERSION     DAJOWB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.060"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(404..826)
                     /locus_tag="OHO85_002680"
     CDS             complement(404..826)
                     /locus_tag="OHO85_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCQ2672036.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDRRMNIICRECRKSEVMQRILAFYKGNIRTLPR"
     gene            complement(867..1832)
                     /locus_tag="OHO85_002681"
     CDS             complement(867..1832)
                     /locus_tag="OHO85_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672037.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDDGKLSESIGSKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDPQTTEQDFLTRNSDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVGLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSSVQRCVNHISEPDTEIPPGFRG"
     gene            complement(1813..2334)
                     /locus_tag="OHO85_002682"
     CDS             complement(1813..2334)
                     /locus_tag="OHO85_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672038.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            complement(2318..2545)
                     /locus_tag="OHO85_002683"
     CDS             complement(2318..2545)
                     /locus_tag="OHO85_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCQ2672039.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKTKRAGRLNNENHS"
     gene            2623..3030
                     /locus_tag="OHO85_002684"
     CDS             2623..3030
                     /locus_tag="OHO85_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2672040.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            3223..3378
                     /locus_tag="OHO85_002685"
     CDS             3223..3378
                     /locus_tag="OHO85_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCQ2672041.1"
                     /translation="MDTIDLGNNEYLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            3380..3955
                     /locus_tag="OHO85_002686"
     CDS             3380..3955
                     /locus_tag="OHO85_002686"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672042.1"
                     /translation="MDFDTIMKKAYEEYFEGLAEGEEALSFSEFKQALRIRMCSHNDA
                     EHKYEKQNQTAENFVLEPGETLFKIPVTCPICGFTSEELDDSCNNQETTKYVEDDTEC
                     ARRTIISTSPNSRTNKSHFERVINPLPQTNKKDAGGQKTQWNNGISVKKRRSIKDKQR
                     SANNCKRFIPVINFHVLKRIYWWLRHCLMNP"
     gene            4435..4623
                     /gene="dicB"
                     /locus_tag="OHO85_002687"
                     /gene_synonym="ftsT"
     CDS             4435..4623
                     /gene="dicB"
                     /locus_tag="OHO85_002687"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HCQ2672043.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKV
                     CIVSMLARLRLQPKGYAQ"
     gene            4620..4811
                     /locus_tag="OHO85_002688"
     CDS             4620..4811
                     /locus_tag="OHO85_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HCQ2672044.1"
                     /translation="MNPVFALILTVFLVSGEPVDIAVSVHRTMQECMAAATEQKIPGN
                     CYPVDKVIYQNNNEIPAGF"
     gene            4902..7340
                     /locus_tag="OHO85_002689"
     CDS             4902..7340
                     /locus_tag="OHO85_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCQ2672045.1"
                     /translation="MSTDKEEFALYCEAKNDKVRKRLGIKGGFYWTTAKKLSVAISRC
                     ITAMDDNDYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGKDGITMTFIGPS
                     PSVQDKPASTDNTNINGEDMTEIEESMLLPVSGQELPIRWLAQHGSKKPVTHVSRDEL
                     QALHIARAEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDKVFPNPGNSSLGPMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPNLVHTLDTLD
                     VEIAAATLPMDFNIYEIPGSVYRRAKEVVLKKESPFKEWSAALRATPGILDYSRAAIF
                     ALIRNAHPEFYHYPGRLQGYINAYLTETDHENPSKETLTVARHTPEKDILEEINREVV
                     TERETEEKPQPSDAMAGERTTTETMEPDTTEHSQDAQSLDAQSQVSSANQVKVTADEV
                     NKIMQAANINQPDADKLLAASRGEFVAGISDPNDPKWVKGIETRDSVNQNQQESERND
                     QKAEQNSSNALQNEPKTKQPEPEEQQEPEKVCTACGQTGGGNCPDCGVVMGDATYQET
                     FNEENQVEVQENDPEEMEGAEHPHKENPGGNQHHDSDNETGEATDPLINVNGHHEITS
                     TSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRD
                     TIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNTILR
                     RSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYV
                     SVIWQKLIPSQADS"
     gene            7413..7664
                     /locus_tag="OHO85_002690"
     CDS             7413..7664
                     /locus_tag="OHO85_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCQ2672046.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            7699..8979
                     /locus_tag="OHO85_002691"
     CDS             7699..8979
                     /locus_tag="OHO85_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCQ2672047.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RASVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTIPHNEAMKN"
     gene            complement(8981..9109)
                     /locus_tag="OHO85_002692"
     CDS             complement(8981..9109)
                     /locus_tag="OHO85_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCQ2672048.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFKSHTCQLFDKY"
     gene            complement(9167..10186)
                     /locus_tag="OHO85_002693"
     CDS             complement(9167..10186)
                     /locus_tag="OHO85_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HCQ2672049.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(10198..11412)
                     /gene="rspA"
                     /locus_tag="OHO85_002694"
     CDS             complement(10198..11412)
                     /gene="rspA"
                     /locus_tag="OHO85_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCQ2672050.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(11618..11944)
                     /locus_tag="OHO85_002695"
     CDS             complement(11618..11944)
                     /locus_tag="OHO85_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCQ2672051.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            12079..12420
                     /locus_tag="OHO85_002696"
     CDS             12079..12420
                     /locus_tag="OHO85_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCQ2672052.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            12455..13015
                     /gene="speG"
                     /locus_tag="OHO85_002697"
     CDS             12455..13015
                     /gene="speG"
                     /locus_tag="OHO85_002697"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HCQ2672053.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(13018..13728)
                     /locus_tag="OHO85_002698"
     CDS             complement(13018..13728)
                     /locus_tag="OHO85_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCQ2672054.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            13836..14141
                     /locus_tag="OHO85_002699"
     CDS             13836..14141
                     /locus_tag="OHO85_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCQ2672055.1"
                     /translation="MKLSTCCAALLLALASPVVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            14340..16766
                     /locus_tag="OHO85_002700"
     CDS             14340..16766
                     /locus_tag="OHO85_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HCQ2672056.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAATV
                     QQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGTRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            16827..19250
                     /locus_tag="OHO85_002701"
     CDS             16827..19250
                     /locus_tag="OHO85_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HCQ2672057.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGKEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            19261..19878
                     /gene="dmsB"
                     /locus_tag="OHO85_002702"
     CDS             19261..19878
                     /gene="dmsB"
                     /locus_tag="OHO85_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCQ2672058.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            19880..20734
                     /locus_tag="OHO85_002703"
     CDS             19880..20734
                     /locus_tag="OHO85_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCQ2672059.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLACIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWVCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            20777..21391
                     /gene="dmsD"
                     /locus_tag="OHO85_002704"
     CDS             20777..21391
                     /gene="dmsD"
                     /locus_tag="OHO85_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCQ2672060.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            21550..22842
                     /gene="clcB"
                     /locus_tag="OHO85_002705"
     CDS             21550..22842
                     /gene="clcB"
                     /locus_tag="OHO85_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCQ2672061.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFHHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVGLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(22795..23490)
                     /gene="bioD"
                     /locus_tag="OHO85_002706"
     CDS             complement(22795..23490)
                     /gene="bioD"
                     /locus_tag="OHO85_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCQ2672062.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(23615..24835)
                     /gene="mlc"
                     /locus_tag="OHO85_002707"
                     /gene_synonym="dgsA"
     CDS             complement(23615..24835)
                     /gene="mlc"
                     /locus_tag="OHO85_002707"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCQ2672063.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(24970..>25811)
                     /locus_tag="OHO85_002708"
     CDS             complement(24970..>25811)
                     /locus_tag="OHO85_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2672064.1"
                     /translation="HFGRAAARLNISQPPLSQQIQALEQQIGARLLARTNRSVLLTAA
                     GKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSAPFIRAVSDTLSLFRRDY
                     PDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHAVIVHEPLMAMIPHDHPL
                     ANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLTPVITQEVGEAMTIIGLV
                     SAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPKHHEQSPAARNFRIHLLN
                     ALR"
BASE COUNT         6897 a         6443 c         6093 g         6378 t
ORIGIN      
        1 tgttggaata atcttgagta gcgtaacgcg atacgccgca gtttggaagt aatatacatt
       61 ccgtttgatt acactcgata ttacagactt gcatgcaaag aaatccaagc tgttaggtaa
      121 atagagaccg cagaggaaag tgatatagtc tataaaaacc aacgaatagc ggcatgtctt
      181 ctgcgacctc catttaccca aatagcgctt atctagtaaa attcaatgat gtattgcata
      241 gtatttcgta tgaattgata tagataagaa tcagtcttaa ttcattagca aatattgaaa
      301 ctcaaaatgt ctgtttttga tacaaagctg acaaccagac cagttctagc catgtgccat
      361 caaaatgtca attcatatct gaagtaatgc tctttaattt cgttcaccgt ggtaatgttc
      421 gaatatttcc cttataaaat gccaaaatcc gctgcataac ttcgctcttc cggcactcgc
      481 gacagattat gttcatacgc ctgtcgtagc gacgtatttc tccgtcaggt aatgaccaga
      541 taaggtccgg atcaaccaca gcaggtttct tcacctttgc cctcgagagt tttttgcggg
      601 cgttttgcca gtccttacgc gcctgttcag aggggaataa cccgtagccg gagttgtata
      661 catcgccact ggcaaccagc tctctggcaa gaacgctcat cagatatctt gtcgcacctg
      721 ttttagcttc cagttgccgt agcgtctcac gaccgctctg gcgcacgagt tccaccacct
      781 gccctttaat tttttcccgc tcttcttgtg taaaaacttt tgccacaagt cccccttaaa
      841 attacctcat gacctgaaat caacacttat cccctgaaac caggcggaat ttctgtatcc
      901 ggttcagaaa tatgattcac acaacgctgt acagacgaac gccccagacg gatgaccagt
      961 tcatcccatt tttcacgaag ttttgacggg ctcatgatgt tttttaccca gaatggatct
     1021 cgctgtaccc gaccaaacat ttcgcaaatt tgtctgtggc ttctgccatc cagcatccgc
     1081 attgtgcgca cgtcattggc ccaggcagtc cagttaggct cttttggtcg catgatctcg
     1141 ccatcatcac tggctgcctg ttcgtagaga cccacgatcc gcccccagat ccactgcgca
     1201 cacgccaaat cctccctgct accccactgg cgttttttcg cactaaacac aaccgcgtcg
     1261 gagttccggg ttaaaaaatc ctgttcagtc gtctgcgggt ccggttgcga agcttccgga
     1321 cgaaaagtgt ttttattctc tgtagtaatc tctgttgtat tctctgtaag atcatcaggc
     1381 cattttgacc cgatgacatt gggtcgtttt gaaccaatgg agcgtttcat tttgacctct
     1441 tccatcgtgt cattttgacc tgatggagcg gcgcattttg aaccgatgga ttcgctcaat
     1501 ttgccatcat ctaaaagctc actcccgtag ttgattgtgt agaaattggt catatcgcgc
     1561 tttgatttat tgagcttttc acaacgcaaa agccccagcg ttttcagact tgcaaacgcg
     1621 cgctttaacg ttgactctga ccagaatggg aactgttcca gccattgttc cgttgtgttg
     1681 taaatccagc gaacaccatc acattccatg ccggaattgg tatctctcaa ccagtaatgc
     1741 aactgctgca acacaatggc ttcatttaag ccaatcttca tcgcaagctg tgtgtttata
     1801 accagtgggc gttcagcaaa aagaaggctc ataattccat ccagcttttt gttggtattg
     1861 ctgtcgatac gcaagtttga aagcaattgc tttttctata agttcgtcag tttcacgatc
     1921 cactacggca ggatcagcaa aaagcagtcc ggactccacc acatcgccat attctttgtt
     1981 taacccggcg atcatgtacg tgatgctttt tccgtcacta atttcacgat acaacctgaa
     2041 atcattaatc cggatagcct ccataattgc aggcactagc gccgtgaact tttcacgctt
     2101 atccctggtg tcgatagcct tccagcgttc gaatatcttc actcgattaa cgccaagcgc
     2161 tcgctgatca accgcgccac cttcatctgt gacacgctga acatcgatgt tcgggcgctc
     2221 tttcaaagcc cagaatgctt cagtgattaa tatcgtcgcc tgctcctgtg tcattcctgg
     2281 tcgacatatc caggcatcca gagcctcacg agcctgttca ggagtgattt tcattgttca
     2341 accgccccgc ccgcttcgtc ttacgatatt cgtcataaac cttgggatca tactgaagct
     2401 cgccgccaga tgcctcctgt agacgcatcg cgcgaccttc aggaaccagc tcttgccatt
     2461 gagaaacagc agatgggtca acgccagcag ctttcgctac tttggctttc gtaccgtaaa
     2521 aattaattac gtctgattta aacatcgcac ctccaatatt gagttttctc aatgctaatc
     2581 actcaaggaa tctcaagtca agggttatta agatatctaa atatgaacga gaaaacttta
     2641 ggtcaacgca ttagagaaag acgtaagcag gtaggcttaa gtcaaaacgg tttaagcaaa
     2701 gctgctggcg tatctggctc atcaatttca ttatgggaaa gtgaccatac agccccgcgc
     2761 gggcaaaatt tgcatcgcct cgccgaggta ttgcaatgtt caccaacctg gatactgttt
     2821 ggcgacgagg acaaaacacc agctccccct gtttcactcg atagtgcctt agacttatcg
     2881 gaagatgagt tagagatgct gcgtttgtat cgcgcacttc ctaaatcaga gcaacaagca
     2941 caactcagcg aactccgcgc ccgcgttgag aattttaatc gcctgttcac cgagctatta
     3001 gaggctcgca aacgcaacaa gcatcaataa tcatcccttc acaaaatttt aaagccttac
     3061 atttcaatgt attggcttta tcttgcgcca atacttgagt tttctcatca aaaaaccttg
     3121 acgaaaaata atgagaaaac taaattacct ccatcaagac accgcacggt gttctcagca
     3181 aacagttccg ctaccccggc gttaagggga aatgaggtca gcatggatac tatcgatctt
     3241 ggcaacaacg aatatctggt gtacggcgtg tttcccaacc aggacggcac gttcaccgcg
     3301 atgacatata ccaaaagcaa aacgtttaaa accgaaaatg gtgcccgtcg ctggctggaa
     3361 agaaactcag gtgagtgata tggatttcga cacaatcatg aaaaaggctt acgaagaata
     3421 cttcgaaggc cttgccgaag gcgaagaagc tctcagcttc agtgagttta aacaggcact
     3481 cagaataaga atgtgctctc acaatgacgc ggagcacaaa tatgagaagc aaaatcagac
     3541 cgcagaaaat tttgttctgg aacccggtga aacgcttttc aaaattcccg ttacgtgccc
     3601 catttgcggt tttacatcag aagaacttga cgactcctgt aacaatcagg aaacaaccaa
     3661 gtatgtcgaa gatgataccg agtgcgcacg aagaacgatt atatccacga gtccaaactc
     3721 caggaccaat aaatctcact ttgagagggt gattaatcca ctcccccaaa ccaataaaaa
     3781 agatgccgga ggacaaaaaa cccaatggaa caacgggata tctgtcaaaa aaagacgttc
     3841 cattaaagac aaacaacgca gcgccaacaa ctgtaagcgc tttataccag taatcaattt
     3901 tcatgttctt aagcggattt attggtggtt gcgacattgc ttaatgaatc cttaaaactg
     3961 tggtgatttt aaggatacca cctcgcctga cgtggttaaa agcaggcaca taacgcgaaa
     4021 gcgcacggcg aggtagctgg ttcatagata gcctgtcgtt aaattttcgt cgaccgtgcg
     4081 cttccggttg tggcactccg cgaaatggcg cggcggtaag tatggcgggg ttattccttc
     4141 cccgttgagg acaccgggtt gtcaggttga ccatacgctt aagtgacaac cctgccacaa
     4201 cactccatgt tgattggtac ctttggcggc atcagtttca ttgctggctg atgtgcgccc
     4261 tttttaaagt gaattttgtg atgcggtgaa tgcggctgag cgcacgcgga acagttaaaa
     4321 ccaaaaacag tgttatgggt ggattctctg tatccggcgt taattgttaa ctggttaacg
     4381 tcacctggag gcaccaggca ctgcatcaca aagttcattg ttgaggacgc gataatgaaa
     4441 acgttattac caaacgttaa tacgtctgaa ggttgttttg aaattggtgt cactatcagt
     4501 aacccagtat ttactgaaga tgccattaac aagagaaaac aagaacggga gctattaaat
     4561 aaagtatgca ttgtttcaat gctggcccgt ttacgtctgc agccaaaagg atatgcacaa
     4621 tgaatccagt atttgcactt attctgacgg tttttcttgt ttccggagaa ccagttgata
     4681 ttgcagtcag tgttcacaga acaatgcagg aatgtatggc agcagcaacc gaacagaaaa
     4741 ttccaggcaa ctgttatccg gtcgataaag ttatttacca gaataataac gaaatcccgg
     4801 caggatttta aaacagcacc gtaataaata tccagtttca ttcttatatg tcagcaatgg
     4861 cagagatttg ttcaccctta aatctgtaat gaggtttatc aatgagcact gataaagaag
     4921 aatttgcact atattgcgaa gcaaaaaatg acaaagtaag aaaacgcctg ggaattaaag
     4981 gtggttttta ctggactaca gcaaaaaaat tatctgttgc aatctcccgc tgcattaccg
     5041 caatggatga caacgattat gatgaagacg actttaaaaa acccgtccgc gtcaatttgc
     5101 ccgttgttga cgaccttccg ccagaaggcg tgtttgatac ggaattctgc aaccgctatg
     5161 aaaaaggcgg gaaagatggc atcacaatga catttatcgg cccttccccc tcagttcagg
     5221 acaaaccagc cagcactgac aataccaaca tcaacggcga agacatgact gagattgagg
     5281 agagcatgct tctgcctgtt tccggtcagg aactgcccat tcgttggctt gctcaacacg
     5341 gcagcaaaaa accggtaacg cacgtttcac gcgacgaact ccaggcatta cacattgcac
     5401 gggctgaaga actaccggct gttactgccc tggctgtttc ccacaaaacc agcctgctcg
     5461 acccgctgga gattcgcgac ctccacaaac tggtgcgtga tactgacaaa gttttcccta
     5521 atcctggcaa ttcaagcctg gggccgatga ctgctttttt cgaagcatac ctagacgcag
     5581 actacaccga tcgcggtctg ctaacaaaag agtggatgaa aggaaatcgt gtttcgcgta
     5641 tcacccgcac ggcttccggt gctaatgccg gtggcgggaa caaaaccgat cgcaatccga
     5701 atttagtaca caccctcgac acactggatg tggagattgc agcagccaca cttccgatgg
     5761 attttaatat ttatgaaatt ccgggcagcg tttatcgtcg cgcaaaagaa gtcgtcctga
     5821 aaaaagaaag tccgttcaaa gaatggtccg cagcacttcg tgcaaccccg ggtattctgg
     5881 actattcccg cgccgctatt tttgcactta tccgaaacgc acaccctgaa ttttatcact
     5941 acccgggacg ccttcagggg tatatcaacg cctatttgac ggaaactgat cacgagaacc
     6001 ccagcaagga aactctcaca gttgcccggc atacgccgga aaaagatatc ctggaagaaa
     6061 ttaaccgcga ggtggttact gagcgtgaaa cagaagaaaa accacaacca tctgacgcaa
     6121 tggcaggtga acggacaaca actgaaacaa tggaaccgga tacaactgaa catagccagg
     6181 acgcgcagtc gctggatgct cagtcgcagg tgagttccgc taaccaagta aaagtcaccg
     6241 ctgacgaagt aaacaaaatt atgcaggcag ccaatatcaa ccagcctgac gccgataagt
     6301 tgcttgctgc atcacgtggt gaatttgttg cagggattag cgacccgaat gatccgaaat
     6361 gggtgaaggg gattgaaacc cgcgattctg tgaaccagaa ccagcaagaa tcggaacgga
     6421 acgaccagaa agcggaacaa aacagctcaa atgcgttaca aaacgagcca aaaacgaaac
     6481 agcctgaacc agaagagcaa caagaaccgg aaaaagtctg taccgcctgc ggtcagaccg
     6541 gcggcggcaa ctgccctgat tgtggtgtgg tgatgggcga cgcaacatac caggaaacat
     6601 tcaatgaaga gaatcaggtt gaagttcagg aaaatgatcc ggaggaaatg gaaggcgctg
     6661 aacatccaca caaggagaac cctggcggca atcagcatca cgatagcgat aatgaaactg
     6721 gcgaggcgac agatccctta attaatgtga acggtcatca cgaaatcaca tccaccagca
     6781 ggacgtgtga ccatctaatg atcgaccttg aaaccatggg aaaaaatcct gatgccccga
     6841 tcatctcaat aggtgcaata tttttcgatc cgcaaaccgg agatatggga ccggaattta
     6901 gtaagactat cgatctggaa actgctggcg gagtcattga tcgggacacc attaaatggt
     6961 ggcttaagca atcacgcgaa gcgcaatctg ccattatgac cgatgaaatc ccgttagatg
     7021 atgcactgtt acaattgcgg gaatttatcg acgaaaactc cggtgaattt tttgttcagg
     7081 tctggggaaa tggagccaac ttcgacaaca cgattttgcg ccgttcatac gaacggcagg
     7141 ggatcccctg cccgtggcgt tactacaacg atcgcgatgt acgcacaatc gttgagctgg
     7201 ggaaagccat agacttcgat gccagaacgg ctattccatt cgaaggtgag cgccataatg
     7261 cacttgatga cgcccgttac caggcaaaat acgtttcagt tatctggcaa aaactgatcc
     7321 cgagtcaggc tgattcttaa tgttcaactg tcgccggttg tgactggtat tctgcaaccg
     7381 gcgctcgtct gatgtaagag ataaagaaat cgatgagcga agtaatcatg attgtctctc
     7441 ccggcaaatg ggtatccgaa gagcagttaa ttgcgctgaa aggaataaaa aaaggtacgt
     7501 taaaaaaggc ccgggaaaaa tcgtttatgg aaggaaggga atataagcat gtcgctcatg
     7561 atggtatgcc atgggataac agtccatgct tttacaacct ggaagaaatt gatcgctgga
     7621 ttgagcgcca ggcatcagca agaccaagac gtcatcttac ttgactaaaa gccacactaa
     7681 ctaatgagag aagttgaaat gaaatatccg acaggcgtgg aaaaccatgg agggaaatta
     7741 cgtatctggt ttgtttataa aggcgtaaga gtcagggaaa acctgggggt tcctgacaca
     7801 gcaaaaaaca ggcgcgttgc aggtgaacta cgcgcctctg tttgttacgc aataaaaact
     7861 ggtgttttcg actatgcaaa acagtttccc tcctcacgca atctggaaaa atttggtgag
     7921 gcccgacaag atttaaccat aaaagaactg gctgaaaaat ttctggcact gaaagaaact
     7981 gaagttgcca aaacatcact caacacatac cgtgccgtca tcaaaaatat cctgagcata
     8041 atcggtgaaa aaaatcttgc ctcatcgatt aataaagaaa aattactgga ggttcgtaaa
     8101 gagctactga ctggatacca gatccccaaa agtaactata ttgttacaca accagggaga
     8161 tcggctgtaa cagtaaataa ttacatgaca aatcttaacg ccgtgttcca gtttggtgtt
     8221 gataacggtt acctggcaga taacccgttt aaggggatct cgccattaaa ggaatcaaga
     8281 accattccgg atcctctttc gcgggaggaa ttcatccgtc ttatcgatgc gtgcagaaat
     8341 cagcaagcca aaaatttatg gtgtgtttct gtttatactg gcgttcgccc tggtgagctg
     8401 tgtgcacttg gatgggagga catagatctg aaaaatggaa caatgatgat caggagaaat
     8461 ttagcaaaag accgtttcac ggtaccaaaa acacaggcgg gaaccaatcg ggtcattcat
     8521 cttattaagc cagcaatcga cgctctccgg agtcagatga cattaacgag actgagcaaa
     8581 gagcatatca ttgatgttca cctcagagag tatggcagaa cagaaaaaca aaaatgcacc
     8641 tttgtttttc aacctgaagt atcagcgaga gtaaaaaatt atggtgacca ttttaccgtt
     8701 gactcaataa ggcagatgtg ggacgcagcg ataaaacgtg ccggactccg ccatcgaaaa
     8761 tcatatcagt cgagacatac ttatgcctgc tggtcgctga ctgctggtgc taacccggca
     8821 tttatagcaa accagatggg ccatgcagat gcgcaaatgg tatttcaggt atacggaaaa
     8881 tggatgtctg aaaacaataa tgcacaggta gctttgttaa atacacagtt aagcgagttt
     8941 gccccaacca taccccataa cgaagcaatg aaaaattaat ttaatattta tcaaatagtt
     9001 gacacgtatg actcttgaaa tccataaatt caagcgcagt gcccagccat cccgatactg
     9061 ctgctttcac caaatcctta gtgcttcttt cgtgtttttc tattgtcata atggttatct
     9121 ctaaaaaaga ggtaagatgc gtactactta ctcgccgtta ttggtattat tcagaaaaag
     9181 tgagtaagac tttgcagcaa tgtttttgat cctgttcaaa taaactaatg gcatcagcaa
     9241 catgctggaa atcaaacgta tgggtaatta atttttctgg tttaattaac cctttactta
     9301 accagtcgat aacaaccgga aatttatttg catttaagcg tgaagagaaa atagagagtt
     9361 cttttccggt aattccttgc tgaatcactt cagacggttc actggagaag cccatcaata
     9421 caatacgtgc cgctggagaa gccagcgtta cggcctcttt caggatagaa ggatgacaag
     9481 ccgcatcgat aattaatgtc ggcttgatgc ctttttcagc gaaaatctcg ccaagcggtg
     9541 tctggctgtt attaatcgcc cagtctgccc cgctctcttt cgctttttcc agtcgttcat
     9601 caatgcgatc ggcaacaatc acatttttaa cgttatagac gccttttaat acctgaacga
     9661 tcgtcaggcc gattggaccg gcaccataaa ccagaacggt atcattttca gtcggttgac
     9721 catgaccggt aacgttagcc gcaatggtaa aaggttcgat catcaccgca tattgatcgg
     9781 ccactgcttc aggaattttc cacgcatttt ttgccggaac cacggcatat tcactgaaac
     9841 caccgtcagc gtgcacacct aatacagcaa gtgtcgtaca aacgttcggc ttacctatag
     9901 agcacggata gcaatgccca cagctgacca ccggatcgac agcaacgcgt tcaccgactc
     9961 tggcgctttc cacaccttca cccaccgcat caatgacgcc aaagaattca tgaccaatga
    10021 cgcgcggata tttcgcaaaa ggattatgcc cacggtaaat atggctatct gaaccacaaa
    10081 ttccggcaag tttcactttt actcgtactt cacccgctga cggggtgggt atttcacgtt
    10141 cgataatcga cagttgattc ggtttttcaa ttaaaatgct tttcattacc ttactcctta
    10201 ccagttccac agcgtgccat cttccagacg tgcgactggc agataagcag gttcataggg
    10261 atatttcgcc gccagctttt catcgaattc gatgccaaga cccggtttgt ctcccggatg
    10321 catatagccg ttatcgaaag tccagttgtg cgggaagact tcgagcattt gttcggaata
    10381 acccatgtac tcctggacac cgaaattggg gacccacaga tcaaagtgca gcgccgcagc
    10441 catgcagact ggtgacaaat cggaaggacc gtgtgagcca gtacgtacct gatacagcga
    10501 agcaaaatcg gcaatccggc gcataccggt aattccgcct gcatgggtca gcgtggtgcg
    10561 gatataatcg atgagttgct cttcaatcag ttgtttgcag tcccagatgc tgttgaagac
    10621 ttcacccact gcgatgggtg tgacggtatg ttggcgaatg agacggaagc attcctggtt
    10681 ttccgcaggc gtcgggtctt ccatccagaa catgcgataa tcttcaatgc ttttaccaaa
    10741 gcgcgccgct tcaataggcg ttaagcgatg gtgcatgtca tgcagcaaat gttcattaaa
    10801 accaaacttg ttacgtaccg cgtcaaacaa tttcggcatg aaatcgaggt atttctccgt
    10861 cgaccacagc tgctcttccg gccactgtcc tttggttgcg ggttcataag ccagaccttt
    10921 acctttcgac atgccgtagg tggttttcat accagggatt ccgcactgca cgcggatggc
    10981 tttgaatccc agctcctgat gacgggcata atcatccaga gcttcatcaa tactgtgacc
    11041 ggtggtatgg caataaacca tcaccccttc acgagacgcg ccgccgagta actggtaaag
    11101 cggcatgttg gcagctttgg ctttaatatc ccacagcgcc atatcaaccg ctgaaatggc
    11161 cgacatcgta accggaccgc gacgccagta agcaccttta tagaaaaact gccagatatc
    11221 ttcgatacgg tgcgcatcgc gaccaataag ctgcggacaa aggtgatcct gcaaataaga
    11281 ggccacggaa agctcacgtc cattgagggt ggcatcccca aggcccgtaa taccgtcctc
    11341 agtggtgatt tttaatgtga cgaaattacg ccccggacag gtaacaaaaa cttcagcctt
    11401 tacgatcttc atgttcgatt ccttgcatcg cttgtcgtga tgcatgaaat ctacgcaact
    11461 gagctactac catacaagta tgaagatcga aaaaagccgg agtgatcaca aaaaaaggcg
    11521 tatatttgcg ctgtgaatgg ttgacaaaag atgaaataga ataccttttg tcagctgaca
    11581 cttcctctta tcttattgat aaaatggatt tatgttccta cgtgcgcccc cagcccgcaa
    11641 caatgatcaa catgccgcaa agcgcaatca acgcacccgt ccagtcataa agagtcagtt
    11701 tcacgccatc cacaacgcgc agccacatca acgccgtgca gacataaacg ccaccataag
    11761 ccgcgtaaac acgcccactc gccgctggat gcaacgttaa caaccagaca aacagcgcca
    11821 gtgaaatccc cgccggaagc aacagccaga tactggcgtt tcgttttaac cacaaccagg
    11881 gcagaaagca tccaataatt tcacacagcg cagtagcaaa aaatagtaac gttgttttaa
    11941 tcatctttgt ctcttattga catcatgtat agttataggg cgacataata tcatcaatat
    12001 aaacaccctc ctggtacgtt ttgcgtccgc agtggatggt gtagaatcac ctttaatcat
    12061 tcatacaggg aatgaattat gaaaatcact ctcagcaaac gaatcggcct gctcgctatt
    12121 ctgctgcctt gcgcactggc attgagcaca actgttcatg ccgaaactaa caaactggtg
    12181 attgagtctg gcgacagtgc acaaagccgc cagcacgccg ctatggaaaa agagcaatgg
    12241 aatgacacgc gcaatctgcg ccagaaagtg aataaacgca ctgaaaaaga gtgggataaa
    12301 gccgacgccg cttttgataa ccgcgataaa tgtgagcaaa gcgccaacat caatgcctac
    12361 tgggagccca atactttgcg ctgcctggac cgtcgaactg gccgcgttat taccccctaa
    12421 cctgttattg atttaaggaa tgtaaggaca cgttatgcca agcgcccaca gtgttaagct
    12481 acgcccgctg gagcgtgaag atttacgcta tgtacatcaa ctcgacaaca acgccagtgt
    12541 gatgcgttac tggtttgagg aaccctacga agcctttgtt gaactctctg atctgtatga
    12601 taagcatatt cacgatcaga gcgaacggcg ctttgtggtg gaatgtgacg gcgaaaaagc
    12661 cggtctggtg gagctggtgg aaattaacca tgttcatcgc cgcgcagaat ttcagataat
    12721 tatctccccg gagtatcagg ggaaaggtct ggcaacccgt gccgccaaat tagcaatgga
    12781 ctatggcttt accgttctca atctctataa gctgtatctg atcgttgata aagagaatga
    12841 aaaagcgatt cacatttacc gcaagcttgg cttttcggtt gaaggtgaat tgatgcacga
    12901 gttctttatt aatggtcaat atcgtaatgc cattcgcatg tgtatattcc agcatcagta
    12961 tctggcagag cacaaaacac cgggtcagac tctcctgaag ccgaccgcac aatagcatta
    13021 ataataatcg atcgtatttt tgatggtgta aacccgttcg acggcgggtt ttactccttc
    13081 atcaacaatg attagctgac agtccaccgg attagcgtga ctgtcatatt cacagctctg
    13141 ttttacatta ccaacccgtt gattattcag taaagtaacc gctgtgtaat ctaatttttt
    13201 gatcggatcc gttgatggcg tggcgctgac agataatgtt ttgtcgttac ttttcgtggt
    13261 tttacccagc ggataaccct gatcatcata gcgatattcc atctgcattt gtttgctgct
    13321 ggctttaatc acgaagccat tatcatcggt ttcccaactc accccggcag aaggtaattc
    13381 tgctagctgg cattttccct gtaaacgtac tctcttctcc agcgtctcgg catcacggta
    13441 ataattggcg tccagtacca gagcgaccac ggtattattt tccagatcca gtaattcgag
    13501 tgaatcaaaa cagccttctt ccgacaaagt cccagaaaca cgtttcgtca cttcaccttg
    13561 ctcatccatt aatgtctgag tgaaatcttt taccggacca cgcagcggat caaaatcgaa
    13621 ttcattagag aaactggcca tctcaggggt aaatgaaagt gttacctctg tgcggtcaca
    13681 tcctgtgagg aatatcgcga gtaagcatgg tagtaatttg tatttcacaa cagtcaccag
    13741 agagtagaga tgattctaaa tcatagtagc aaatacagta ctttacacgt taaatgctat
    13801 gcttaaagaa gttatcttcg cgtaaggagc ttatgatgaa actctcaacc tgctgtgccg
    13861 cgcttctgct cgccctcgcc tcacccgtgg tactcgccgc gccgggatcc tgtgagcgca
    13921 tacagagcga tatatcacag cgcattatca ataatggcgt acccgaaagc agcttcacgt
    13981 taagtattgt acccaatgac caggttgatc agcctgattc ccaggtcgtc ggccattgcg
    14041 ctaatgatac gcataaaatt ctctataccc gcaccaccag cggtaacgtc tctgctcccg
    14101 cgcagtctac ccaggatggc gcgcctgccg aaccgcagta atacattatc gactgaacgc
    14161 cggatacgac acatccggcg atttgaacat acaacataat cccaccttat tactcatacc
    14221 cttctattga tatggattaa taattcttaa cccaaaatgg gtagactccc tctattgtta
    14281 gcgcgctaaa tattcaatat ataaacattt atataacgat aaagaacagg gagtgagtta
    14341 tgtccaaaaa tgaccgaatg gtgggcatca gccgcagaac actcgttaaa tcaaccgcga
    14401 taggttctct ggcgctggct gcaggcggtt tttctttacc gttcaccctg cgcagtgcag
    14461 cggcaacggt acaacaggcc agcgaaaaag tgatctgggg tgcctgttcc gtcaactgtg
    14521 gtagccgctg tgcacttcgt ctacatgtta aagataatga agtgacctgg gtggaaactg
    14581 acaataccgg cagcgatgag tacggcaacc atcaggtacg cgcctgtttg cgcggtcgct
    14641 ccatccgccg gcgtattaat catcccgatc gcttgaatta cccaatgaaa cgcgtgggta
    14701 cacgcggtga aggcaaattc gaacggatta gctgggatga agccctggat actatcgcca
    14761 gtagcctgaa aaaaaccgtc gaacaatatg gcaatgaggc tgtatatatt cagtactctt
    14821 cggggattgt tggcggcaat atgacccgct cttcgccatc agcctcggcg gtcaaacgcc
    14881 tgatgaactg ctacggcggt tcactcaatc agtatggctc ctacagcacc gcgcaaattt
    14941 cctgtgccat gccctacacc tacggcagta atgatggcaa cagcaccacg gatattgaaa
    15001 acagcaagct ggtcgtgatg tttggtaaca acccggcaga aacccgcatg agcggcggtg
    15061 gcatcactta tcttcttgaa aaagcgcgcg agaaatcgaa cgccaaaatg attgttatcg
    15121 atccgcgata taccgatacg gctgctggtc gtgaagatga atggctccct attcgcccgg
    15181 gcaccgatgc cgcgctggtt gcgggtattg cctgggtatt gattaacgaa aatctcgttg
    15241 atcaaccttt tctcgataaa tactgcgtcg gctatgacga aaaaacctta cccgcagatg
    15301 cacccaaaaa tggtcactat aaagcctata ttcttggtga aggtgatgat aacacagcga
    15361 aaacgccgca gtgggcttcg caaattaccg gtatcccggt agaccgtatc atcaaactgg
    15421 cgcgtgaaat tggcacagca aaacccgctt atatctgcca gggctggggg ccgcaacgcc
    15481 aggcaaacgg cgaactgact gcacgcgcta ttgctatgtt acctattttg acgggcaatg
    15541 tcggcatcag cggcggaaat agtggcgcgc gtgaatcgac ctataccatt accatagaac
    15601 gcctgccggt gctggataat ccggtcaaaa cgtcaatctc ctgcttcagc tggacagatg
    15661 ctatcgatca tggtccgcaa atgacggcaa tccgcgacgg cgtccgcggc aaagataaac
    15721 tggatgtgcc cattaagttc atctggaact acgcgggaaa taccctcgtt aatcagcatt
    15781 ctgacatcaa caaaacgcat gaaattctgc aagacgaatc gaaatgcgaa atgattgtgg
    15841 tcatcgaaaa ctttatgacc tcatcggcaa aatatgccga cattctgctg ccagacctga
    15901 tgaccgttga gcaggaagat attattccta acgactacgc cggtaacatg ggatatctca
    15961 ttttcctcca gccagtcacc agcgaaaaat tcgaacgtaa accgatttac tggatcctga
    16021 gtgaagtcgc gaaacgtctt ggacctgacg tctatcaaaa gttcactgaa ggtcgcacgc
    16081 aggaacaatg gttacaacat ctgtacgcca aaatgcttgc caaagatccg gcgttaccgt
    16141 cttacgacga actgaaaaaa atgggtatct ataagcgtaa agatcccaat ggtcattttg
    16201 tcgcctacaa agcatttcgt gacgaccccg aggcaaatcc acttaaaaca ccttccggta
    16261 agattgaaat ttattccagc aagctggcgg aaattgcccg tacctgggaa ctggaaaaag
    16321 atgaagtgat aagcccactg ccagtttatg cctctacctt tgaaggctgg gactcccctg
    16381 aacggagtac cttcccactg caactgtttg gtttccatta caaatcccgt acgcactcga
    16441 cctacggtaa cattgatgtc ctgaaggcag cctgccgtca ggaagtgtgg atcaacccta
    16501 tagatgcaca gaaacgtggg attgccaacg gcgatatggt gcgggtgttt aaccatcgtg
    16561 gcgaagttcg gctaccagcc aaagtgacac cacgcattct ccctggggtt agcgctatgg
    16621 gccagggagc ctggcacgag gccaatatgt ctggcgataa aatcgaccat ggcggctgtg
    16681 tgaatacgct aaccactctg cgcccttcac cactggcgaa gggaaacccg cagcacacta
    16741 atctggtcga gatcgaaaaa atataaccca cgacaaccat aaattctggc atgacatttt
    16801 gtttgaaaag caataagtga gtaatgatga aaatccatac cacagaggcg ctcatgaagg
    16861 ctgaaatcag ccgtagaagt ctgatgaaaa cctccgcact tggcagtctt gcgctggcaa
    16921 gcagtgcttt cactctgcca ttttctcaaa tggtccgggc ggcagaggct ccggtagaag
    16981 agaaagcggt ctggagttcc tgcaccgtta actgcgggag ccgctgtctg ttacgtttgc
    17041 atgtgaaaga tgacaccgtg tactgggtgg agtctgatac gacaggtgac gacgtctacg
    17101 gtaatcatca ggttcgcgcg tgtttacgcg ggcgctctat ccgccgacgg atgaatcatc
    17161 ctgataggtt gaaatatccc atgaagcgcg tcggcaagcg cggtgaaggt aaatttgaac
    17221 ggataagttg ggacgaagcc ctggatacca tcagtgataa tcttcggcgg atcctaaaag
    17281 attacggcaa tgaggctgta catgtcctgt acggaacagg cgtagatggc ggaaacatca
    17341 ccaactcaaa cgtcccgtac cgtctgatga actcttgcgg tggttttctc agtcgctatg
    17401 gcagctacag taccgcacag atcagtgccg caatgagtta tatgttcggt gccaatgatg
    17461 gcaacagccc ggatgatatc gccaatacga aactggtcgt tatgttcgga aataacccgg
    17521 cagaaacgcg gatgagcggc ggtggtgtca cttactacgt cgagcaagcc cgcgaacgtt
    17581 caaacgcacg catgatcgtc atcgatccac gttataacga cacagctgcc gggcgtgaag
    17641 atgaatggct gcccattcgc ccaggcaccg atggcgcact ggcctgtgcg attgcctggg
    17701 tactgattac tgaaaacatg gtcgatcagc catttctcga caaatattgt gttggttacg
    17761 atgaaaaaac tctgcccgcc aacgcaccac gtaacgcgca ttataaagcc tatattctgg
    17821 gcgaagggcc tgacggcata gctaaaacgc cggaatgggc agcaaaaatc accagcatcc
    17881 cggcagaaaa aattatccag ttggcacgag agatcggttc agcaaaacct gcttatattt
    17941 gtcagggttg ggggccacaa cgacattcca acggcgagca aacgtcccgc gctattgcca
    18001 tgctttccgt tctcaccggt aacgtcggca taaacggcgg caactcaggt gtacgcgaag
    18061 gtagctggga tctgggggta gaatggttcc cgatgcttga gaaccctgtt aaaacgcaga
    18121 tttccgtctt tacatggaca gatgccatcg accatggtaa ggaaatgacc gcgacccgtg
    18181 atggtgttcg tggaaaagaa aaactggatg tccccatcaa gtttttatgg tgctacgcca
    18241 gtaacacatt gatcaatcaa catggcgaca tcaatcacac ccatgaggtg cttcaggatg
    18301 acagcaagtg cgagatgatt gttggcattg accacttcat gacggcctcg gctaagtatt
    18361 gcgatatcct gttgcccgac ctgatgccaa cagagcaaga agaccttatc tctcatgaat
    18421 ctgcagggaa tatgggctat gtgatcctcg cccaacccgc aacctctgca aaatttgaac
    18481 gcaaacccat ctactggatg ctgagtgaag tcgccaaacg cttaggacca gacgtttatc
    18541 aaacctttac tgaaggtcgc agtcagcatg aatggatcaa atatctccat gcgaaaacga
    18601 aggaacgtaa ccctgagatg cccgactacg aggagatgaa aacgaccggg atctttaaga
    18661 aaaaatgccc ggaagaacac tacgtcgctt tccgcgcatt ccgtgaagat ccccaggcaa
    18721 acccgttgaa aacaccttcg gggaaaatcg aaatttattc tgaacgactg gcgaagattg
    18781 cggatacctg ggaattgaaa aaagatgaaa ttattcatcc ccttccagct tatacgccag
    18841 gttttgatgg ctgggacgat cccctgcgga aaacctatcc actgcagtta acgggcttcc
    18901 actacaaggc gcgtacccac tccagctacg gcaatattga tgtgttacag caggcctgcc
    18961 cacaagaggt gtggatcaac cccattgatg ctcaggctcg cggtatccgt catggcgata
    19021 ccgtgcgggt atttaacaat aatggagaaa tgctgattgc cgcaaaagtg actccgcgta
    19081 ttctgcctgg cgttaccgcc atcgggcaag gtgcgtggct taaagcggat atgtttggtg
    19141 accgggtcga tcacggcggc agtatcaata ttctgacctc tcaccgccct tcaccgctgg
    19201 caaagggaaa cccgtcgcac agcaatcttg tccagatcga aaaggtttaa agagtagccc
    19261 atgaccacac aatatggatt ttttatcgat tccagccgct gtactggctg taaaacctgc
    19321 gaactggcgt gcaaagattt taaagatctt ggcccggaag tcagtttccg ccgtatttat
    19381 gaatatgctg gcggcgactg gcaggaagat aatggcgtct ggcaccagaa cgtgttcgct
    19441 tattatctct ccatctcctg taaccattgc gatgacccag catgtacaaa agtctgcccc
    19501 agcggcgcaa tgcataagcg tgaagatggc tttgtggtgg ttgatgaaga tgtttgtatc
    19561 ggctgccgct actgccacat ggcttgtccg tacggcgcac cacagtacaa tgctgaaaaa
    19621 gggcacatga cgaagtgcga tggttgttat tcgcgcgtcg ccgaggggaa acaacccata
    19681 tgcgtcgaat cctgcccgct gcgtgcactt gagttcggtc caattgaaga gctgcgccag
    19741 aagcacggca ctctggctgc cgtcgcgcca ctgccgcgcg cgcatttcac aaaacccaat
    19801 atcgttatca aacctaacgc caacagccgc ccgaccggcg ataccactgg ttatctggct
    19861 aatccggagg aagtgtaaaa tgggaaatgg atggcatgaa tggccactgg ttatctttac
    19921 cgtattgggt caatgcgtag taggcgcgct gattgttagc ggaattggct ggttcgccgc
    19981 caaaaatgac gcagaccgtc agcgtattgt ccgtggcatg tttttcctct ggctgttgat
    20041 gggcatcggg tttattgcct ctgtcatgca cctgggatcg ccactgcgag cgtttaactc
    20101 gctgaatcga atcggagctt ctggtctgag taacgagatt gctgccggtt ccatattttt
    20161 tgccgttggc ggactatggt ggctggtggc ggtcatcggt aaaatgccgc aagcgttggg
    20221 gaaactctgg ttacttgtca gcatggcgct cggcgtcatt ttcgtctgga tgatgacctg
    20281 cgtgtatcaa atcgacaccg tgccaacctg gcataacggt tacactacgc tggctttctt
    20341 cctgacggta ttgttgagcg gtccaatact tgctgccgca attctgcggg cagctcgcgt
    20401 tacatttaat accacaccat ttgccatcat tagcgttctg gcgctgatcg cttgtgccgg
    20461 ggtgattgtg ctgcaaggtt tgtctctggc gtgtattcac tcatccgtac aacaagccag
    20521 cgcactggta ccagattacg cctccttaca ggtctggcgt gtggtattgt tatgcgcagg
    20581 tctaggatgc tgggtttgtc cgctcattcg tcgccgtgaa cctcacgttg ccggacttat
    20641 tctggggtta atcctcattc ttggcggtga gatgatcggg cgtgtgctct tttatggcct
    20701 gcacatgacg gtaggaatgg ctattgcagg ttaacaaatt ggtgcggggc aacccgcaca
    20761 tttcaggatg ttaggaatga cccatttttc acagcaagat aatttttctg tcgcggcgcg
    20821 cgtgttgggc gcgctgtttt attacgctcc agagagtgcg gaagccgcac ctctggttgc
    20881 ggtactcacc agtgacggct gggaaactca gtggcctcta ccagaagcgt cattagcgcc
    20941 gctggtgacg gcttttcaga cacagtgtga agagactcac gcccaggcct ggcagcgttt
    21001 gtttgtcggc ccgtgggcac tgccgtctcc gccgtggggg tcggtctggc tggatcgcga
    21061 atctgtgctg tttggcgatt caacattggc acttcgtcag tggatgcgcg agaaaggcat
    21121 tcagtttgaa atgaagcaaa acgaaccgga agatcatttt ggttcactgc tgttgatggc
    21181 tgcgtggttg gcggagaatg gtcgccagac agaatgcgaa gaattgctgg catggcacct
    21241 ttttccgtgg tcaacacgtt ttcttgatgt ttttatcgaa aaagcagaac accctttcta
    21301 ccgtgcactg ggtgaactgg ctcgcttaac gctggcgcaa tggcaatcac aactgttgat
    21361 tcctgtcgcg gttaaaccgc tgtttcgata ggatcaccgt aatattgccg gatggtgacg
    21421 attacgcgca atccggcaat aataggttac agtgtcacgt ttttttatct cttaaagcac
    21481 gcactgcttt tgcggctggc ctcttttgtc gcaaaatagt cgcccgtgtt tcattgccca
    21541 tttctgctca tgcatcatct acacatctat ccggatctgc gcactatgtt ccgccgtctg
    21601 cttatcgcaa cagtcgtcgg tattctcgcg gcctttgccg ttgccgggtt tcatcatgcg
    21661 atgctgctac tggagtggtt gttcctcaat aatgactccg gcagtctggt caatgcagcg
    21721 acaaaccttt ccccctggcg acggctgcta actccggcgc tcggcggact ggcggcgggt
    21781 ttgttgctga tgggctggca gaaatttacc caacaacgcc ctcatgcgcc gaccgattac
    21841 atggaagcgt tgcaaaccga tggacagttc gattacgcag caagcctggt taaatcgctt
    21901 gcctcgctgc tggtagtaac cagcggcagt gcaattggtc gcgaaggtgc gatgattctt
    21961 ttagctgccc ttgccgcctc ctgttttgcc caacgtttta cgccacgcca ggagtggaaa
    22021 ttatggatcg cctgtggggc cgcggcggga atggctgcgg cctatcgtgc cccgcttgct
    22081 ggcagtttat ttatagccga agtgctgttt ggcactatga tgttggcctc tctcggcccg
    22141 gtgattattt ccgccgtcgt ggcattgctg gttagcaatc tgattaatca tagcgacgcg
    22201 ttactctaca acgtacaact ctcagtgacg gttcaggctc gtgactatgc gctgattatc
    22261 agtacaggtg tgctggcagg tctgtgcgga ccactgttgt taacgttaat gaacgcctgt
    22321 catcgtggat ttgtgggtct caaacttgcg ccgccctggc aactggcact aggcgggttg
    22381 atcgtgggtc tgctttccct gttcacacct gcagtgtggg gcaacggcta tagcaccgta
    22441 caatcctttt taaccgcccc gccactgtta atgatcattg ccgggatctt cctttgtaaa
    22501 ctgtgtgccg tgctggcgag tagcggttcc ggcgcacccg gtggtgtctt tacaccgacg
    22561 ctatttatcg gtcttgccat tggcatgttg tatggtcgta gcctgggatt atggttccct
    22621 gatggcgaag aaattacgct tttactcgga ttgaccggga tggcgacact gttggcggca
    22681 accacgcacg cgccgattat gtcgacgttg atgatatgtg aaatgaccgg ggagtatcag
    22741 ctactccccg gtttattgat tgcctgcgta attgcgtcgg taatctcgcg gacgttacac
    22801 cgtgactcta tctaccgcca gcacactgcg cagcatagct aagcggatgt attgccccag
    22861 ttcacgctgt tcggcgcgcg gcagataagg cagttcacca atgagcggtg ccggaagttt
    22921 tttaccgagc acatcaatga tttccgcata atgcgccagt cctgggttga ttcggttagc
    22981 cacccagcca atgagcggca gcccgtcgtt ggcaatcgcc tgagctgtta gcagtgcatg
    23041 gttaatgcaa ccttcctgaa taccgacaac catcaacacc ggcagttgtt cctgcaccac
    23101 ccattcagag agtggacgca aatcattcat cagactgcgc cagccgccag tcccttctac
    23161 cacgacatga tcgacttttt cggtcaggtt tgccaggccg tttgaaatga gggtgtaatt
    23221 gattgggcaa ctgtgcgcca cgctactttc ttcttcgctt aacgcgatag gattaactgc
    23281 ttcataaggc agttcgatgg ttgaaacact ctgcaacacc agggcatctt tattacgcag
    23341 cccttcgggt gtctctttgc tccccttcgc tacgggttta tatcccgcaa ccgtttttcc
    23401 ctgggaggct aacgcttgta gcaatgcgcg ggaaaccacc gttttcccta cagaagtgtc
    23461 tgtaccggta ataaagaaac gcttcagcat cactaactcc accgttatgc ttcacaaata
    23521 taaaccagga aaataattaa ccttgaaagt ctaagttatg ctttcctggc ccaaattgag
    23581 atagcgcaaa ttttggtaga acagttaaaa aatgttaacc ctgcaacaga cgaatcaaca
    23641 aagaaccgtt atacatcgcg tcttttacca gcgcagcgcc tgccatcgtg ccctggttag
    23701 aaaactgagt actctcaacg ctaatgtgct gactatacgc aggaagggcc tgctgacgga
    23761 tgctgtctga gatgaccggg aagaggatat ctgccgcttt acttaacggt gagccaatca
    23821 gtattttttg tgggttaaat aaattcacca tgatggcaag aatgcgcccc acatgcgcgc
    23881 ccaccccggt aatgatgtct tttgccagca gatcgccgcg caatgccgcc tgacacaatg
    23941 agtccacggt taacggttgc ccatgtaaca tcgagctcat ggattgattg agacgcagct
    24001 gtgccagctc aagtatactg tccacactgg cgatggtttc gaggcagccg tgattcccgc
    24061 aataacaacg tttcccatac gggtcgacct gtgtgtggcc tatttccacg aggctactgc
    24121 tgcctgcgtg tagcagatga ccatcggtaa tgacgcccgc ccccacgttg tgatcgataa
    24181 ccacctgaat cacatcgcgc gccccgcgtg aggcaccaaa caaggcctct gccatcgtcc
    24241 atgcgctgat atcatgctga atataaaccg gaacgccggt atgctgctcc agagcctcgc
    24301 cgagcggcat ctcttttaca tcctcgtaga acggcatgcg atgtacaatg ccattttccg
    24361 tatcaataat tcccggcagg gttatggcaa tcgaagttag acgctcaagt tttttctggt
    24421 ggcggataaa aaactgatcg atatgggaaa tgatacgatc cagcaatggc gagtcatctt
    24481 ttaacgccag ttcctgcgac tcttccacca ccagtttgct gctcagatcg cgcagagcaa
    24541 ggaaaatctc cccgcgacta atgcgcagag aaagatagtg ccaggcttca gtttcaacca
    24601 ccagccccac cgccggacgg ccacggttcc ccgcttcttt gatttccagc tcttgcacca
    24661 ggtgtgcttc gagcatctca cggacaattt tagtgatact ggcaggagcc agttgcgcca
    24721 gacgggaaag atcgatacgc gagactggac caagctgatc aatcaggcga taaaccgcgc
    24781 ccgcgttggt ctgctttatt tgatcaatgt gcccaggctg gttttcagca accaccgcat
    24841 actccctata ttttcgcgct ccgaaataat ctgtaggcta tggtgaagca cttcaatacg
    24901 tgtcgtcaaa tttttactta ggcatgtgat taacagcaca tttttcgggc tttttcgctg
    24961 aaatttccct cacctgagag cattcagcag atgaatacga aagttacgcg cagctggact
    25021 ttgttcatga tgtttcggcc agaccaacca catttcagaa accgcatcct cttctgcaat
    25081 cggcacccag cgcatttcgt tgagctgaac acgtttaaat gacgcaggca aaattgaaac
    25141 acccagaccg gcggaaacca gaccgatgat ggtcattgcc tcgcccacct cctgagtgat
    25201 gacgggcgtc aaatggtaac gtcgcatcag cccgagaata tcgtcataca gccctgtccc
    25261 gacgtgcgga tcaaaaaaga cgaagggttc tttcgccagt tcagccagcg ttacattcgg
    25321 gttatttgcc aggggatgat cgtgcgggat catcgccata agcggttcat ggacgatgac
    25381 tgcgtgttca agcgtctccg gtaacgctgt gttacgcagc aatcccatat ccagcgttcc
    25441 ttcaatgagc ggagcgattt gctcacgagt gttcatttcg cgggtttgta aatggacatc
    25501 gggataatca cggcgaaaca gcgataacgt atcagacacc gcccgaataa aaggagcgga
    25561 cgaagtaaaa ccaatacgca actcccccgc ttcaccctga tgcagtcttt cagcgcgagc
    25621 ggcagcgtca tccaccatag acaggatttg ccgactatct gcaagaaact gttttcctgc
    25681 tgccgtcagc aatacactgc gattagttcg tgccagcagt cgggcaccga tttgttgctc
    25741 cagtgcctga atctgctgac ttagcggcgg ttgcgaaata ttcaggcggg cagccgcgcg
    25801 cccaaaatgc a
//
LOCUS       DAJOWB010000061        25020 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJOWB010000061 DAJOWB010000000
VERSION     DAJOWB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.061"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..614
                     /locus_tag="OHO85_002709"
                     /pseudo
     CDS             1..614
                     /locus_tag="OHO85_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278649.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
     gene            657..1958
                     /locus_tag="OHO85_002710"
     CDS             657..1958
                     /locus_tag="OHO85_002710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672065.1"
                     /translation="MLNKKLNMRVLRHSLMIASTISVLTGCDYFTDNWEIRNGACQAI
                     SSEGNETALISDEGMVFMSMDKGCPDNLDFPGTRFITTRINDQTWKSLMVCNSATGFR
                     RMVTLGDTPRAAPDMAARAIPAFTHFMGATVTIEGKTATFRKGNFTEACKTFIPKSLS
                     HTDARKNKKMILDEFLYSGDRQPHSPETEITSLQERMMTESGYEKVNGEWRKKSRMPT
                     GGYTNEQAGYSSTTKRFNDADNNPPDTHIQAPAYDATVTSPGKTDTGSGKQNPPEPEK
                     ARPAAPDSPSAIPATALADCTLTNGKALSALAQDGFPYTYHLLKKDGTLELELKEGMF
                     GVSAFHYWRDLNRGKGKLKYLRFHKGSYDYVVVQRYDGETPAFDGILVFNQGTRIATL
                     NCRNTFSELFAIEQFPDNSHKDNEDMADFIEGYTVRGEAVR"
     gene            2021..2557
                     /locus_tag="OHO85_002711"
     CDS             2021..2557
                     /locus_tag="OHO85_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HCQ2672066.1"
                     /translation="MNDSTISSLKEKLNTSTWHLVLLGAATYGVYPLMWLYKYQDTIM
                     DETRQRFSSSAMIIWMAVCLGISAMLKMAFPVQIDEYGDVYGYSRGAETMYGIATLVS
                     LVWIILTIIWAFRARAALQQYALTQFRFELKMNPVWTFLFHIFYINYCINAMPESLAK
                     HRIIYGEPDSRPRDNSPQ"
     gene            2633..2854
                     /locus_tag="OHO85_002712"
     CDS             2633..2854
                     /locus_tag="OHO85_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCQ2672067.1"
                     /translation="MFCLLSEGPFSREQAVAITTAYRNVFIENDQGTHFRLVIHNAEC
                     QLRWRCWNFEYDAGKQLNAWLASEGLPRQ"
     gene            2945..3763
                     /locus_tag="OHO85_002713"
     CDS             2945..3763
                     /locus_tag="OHO85_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCQ2672068.1"
                     /translation="MRLASRFGHVNQIRRDRPLTHEELMHYVPSIFGEDRHTSRSERY
                     AYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGDVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTTDILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            3855..4340
                     /locus_tag="OHO85_002714"
     CDS             3855..4340
                     /locus_tag="OHO85_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCQ2672069.1"
                     /translation="MTTFSHSSTTPSVSVTTASGNNQSQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDGENWVLFNA
                     MNGNRAEMSPEAAGIAACLMAYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            4356..4832
                     /locus_tag="OHO85_002715"
     CDS             4356..4832
                     /locus_tag="OHO85_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCQ2672070.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKALDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLITGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVIPSKADRLITERLVQALALVDIRVPDHLIIGGSQVFSFAEHGLL"
     gene            4919..5011
                     /locus_tag="OHO85_002716"
     CDS             4919..5011
                     /locus_tag="OHO85_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001418935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HCQ2672071.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYR"
     gene            5015..5140
                     /locus_tag="OHO85_002717"
                     /pseudo
     CDS             5015..5140
                     /locus_tag="OHO85_002717"
                     /inference="COORDINATES: protein motif:HMM:NF017938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
     gene            5220..5588
                     /locus_tag="OHO85_002718"
     CDS             5220..5588
                     /locus_tag="OHO85_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCQ2672072.1"
                     /translation="MSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRACIRGRFSDADSYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            5678..6055
                     /locus_tag="OHO85_002719"
     CDS             5678..6055
                     /locus_tag="OHO85_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCQ2672073.1"
                     /translation="MKTLPDTHVREASCCPSPVTIWQTLLTRLLDQHYGLALNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPGAKQ"
     gene            6052..6201
                     /locus_tag="OHO85_002720"
                     /pseudo
     CDS             6052..6201
                     /locus_tag="OHO85_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6277..6474
                     /locus_tag="OHO85_002721"
     CDS             6277..6474
                     /locus_tag="OHO85_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672074.1"
                     /translation="MMPLTTETALDILIAWLQDNIDCESEIIFDNDGDKTDSAALLPC
                     IEQAREDIRTLRQLQLLYQNR"
     gene            6559..7425
                     /locus_tag="OHO85_002722"
     CDS             6559..7425
                     /locus_tag="OHO85_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCQ2672075.1"
                     /translation="MSEITAPRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTARDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEICEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLNNGPQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIARHYPE
                     ALASTVNKSSKA"
     gene            7497..7760
                     /locus_tag="OHO85_002723"
     CDS             7497..7760
                     /locus_tag="OHO85_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HCQ2672076.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            7757..8083
                     /locus_tag="OHO85_002724"
     CDS             7757..8083
                     /locus_tag="OHO85_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCQ2672077.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(8497..9033)
                     /gene="yghD"
                     /locus_tag="OHO85_002725"
     CDS             complement(8497..9033)
                     /gene="yghD"
                     /locus_tag="OHO85_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HCQ2672078.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETILQQLVAMNTRLKSAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIRRIAERGENIQVWIEPVVFNDLLKWLNALDEKYALRVTQIDVS
                     AAEKPGMVNVQRLEFGRG"
     gene            complement(9035..10213)
                     /gene="gspL"
                     /locus_tag="OHO85_002726"
     CDS             complement(9035..10213)
                     /gene="gspL"
                     /locus_tag="OHO85_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HCQ2672079.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPVVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADFVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(10210..11187)
                     /gene="gspK"
                     /locus_tag="OHO85_002727"
     CDS             complement(10210..11187)
                     /gene="gspK"
                     /locus_tag="OHO85_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HCQ2672080.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALITRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(11190..11789)
                     /gene="gspJ"
                     /locus_tag="OHO85_002728"
     CDS             complement(11190..11789)
                     /gene="gspJ"
                     /locus_tag="OHO85_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HCQ2672081.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(11786..12157)
                     /gene="gspI"
                     /locus_tag="OHO85_002729"
     CDS             complement(11786..12157)
                     /gene="gspI"
                     /locus_tag="OHO85_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HCQ2672082.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQQILEEKTVA
                     GWVAENQTALLYLMTREQRAVRQQGESDMAGSRWYWRTTPLSTGNALLQAVDIEVSLH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(12154..12717)
                     /gene="gspH"
                     /locus_tag="OHO85_002730"
     CDS             complement(12154..12717)
                     /gene="gspH"
                     /locus_tag="OHO85_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HCQ2672083.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASSGVVQTFATDSEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDAPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(12721..13176)
                     /gene="gspG"
                     /locus_tag="OHO85_002731"
     CDS             complement(12721..13176)
                     /gene="gspG"
                     /locus_tag="OHO85_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HCQ2672084.1"
                     /translation="MYSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(13193..14416)
                     /gene="gspF"
                     /locus_tag="OHO85_002732"
     CDS             complement(13193..14416)
                     /gene="gspF"
                     /locus_tag="OHO85_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HCQ2672085.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDALQASGVYWLAGLLGLLVLGQRLLKNPAMRLRWDQTVLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(14416..15909)
                     /gene="gspE"
                     /locus_tag="OHO85_002733"
     CDS             complement(14416..15909)
                     /gene="gspE"
                     /locus_tag="OHO85_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HCQ2672086.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(15909..17969)
                     /gene="gspD"
                     /locus_tag="OHO85_002734"
     CDS             complement(15909..17969)
                     /gene="gspD"
                     /locus_tag="OHO85_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HCQ2672087.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREIVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(17999..18958)
                     /gene="gspC"
                     /locus_tag="OHO85_002735"
     CDS             complement(17999..18958)
                     /gene="gspC"
                     /locus_tag="OHO85_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HCQ2672088.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSFNTDKEHLRKIVRGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEGHSTVAVTNKKAVSDEAKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(18976..19386)
                     /gene="gspS2"
                     /locus_tag="OHO85_002736"
     CDS             complement(18976..19386)
                     /gene="gspS2"
                     /locus_tag="OHO85_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HCQ2672089.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(19452..20261)
                     /gene="pppA"
                     /locus_tag="OHO85_002737"
     CDS             complement(19452..20261)
                     /gene="pppA"
                     /locus_tag="OHO85_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HCQ2672090.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFIAFYSLRWIAGVVLRKEALGMGDVLLFAALGSW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATIYLQALF"
     gene            complement(20409..24974)
                     /gene="sslE"
                     /locus_tag="OHO85_002738"
                     /pseudo
     CDS             complement(20409..24974)
                     /gene="sslE"
                     /locus_tag="OHO85_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
BASE COUNT         5860 a         6966 c         6250 g         5944 t
ORIGIN      
        1 tggccggaga atctctgtca ctaaaaacac tgtcagatga atttggcgtt acagaacgta
       61 ctttacagcg cgattttcat tagcgtctgg ttcacctgga tttagagtac agaaatggca
      121 ggtacagcct cagacgacag agcagcccag gtgcgatccc tgaaatgctt tcttttatac
      181 agaataccgg gatcgcacgg atacttccgc tccggaacgg acgactgata acctgtctta
      241 ccgacaacca ggagccctct ccctgcctta tctggctacc ggcgccggat atcactgcaa
      301 cactcccaga gtgtttctcg caactcatcc tggcaataag acagtgcatc cacatctcgc
      361 tggtgactga gcgatggtat ccgtcactgg aaccctgccg gctcatttat tacagcggaa
      421 gctggtatct gatcgcgtta cagaagggaa aactgcgggt ctttcccctg gcagacatca
      481 aatcggtcag cctgacatca gaacggtttg aacggagaga ccacatccac agtctggtcg
      541 ctgaagagcg ttttatctcc gctctgccac atttcccttt catccataaa cttatcaaca
      601 cctttaacct gtgatcgccg gcctgccaaa gccgttccga caggtatgga gacaatatgc
      661 tgaacaaaaa actgaacatg cgggtattac gtcattccct gatgatagct tcgacaatat
      721 ccgttcttac cggttgcgac tattttaccg acaactggga aatcagaaac ggcgcatgcc
      781 aggccattag tagtgaagga aatgaaacag ccctcatttc tgatgaaggt atggtcttta
      841 tgagcatgga caagggatgt ccggataatc tcgattttcc gggaacccgc tttatcacta
      901 ccagaatcaa cgaccagacc tggaaatctc tcatggtatg taacagcgcc acgggatttc
      961 gcagaatggt gacgctgggg gatacccccc gtgcagcgcc ggatatggca gcccgtgcca
     1021 taccggcttt cacacacttc atgggagcaa ccgttaccat tgaagggaaa acggccactt
     1081 tccgtaaagg taacttcacc gaagcctgta agacatttat tccgaaatca ctcagccaca
     1141 cggatgcccg gaagaacaaa aagatgatac tggacgagtt tctgtattcc ggagacaggc
     1201 aaccgcactc ccctgaaaca gaaatcacca gtctgcagga gcggatgatg acagaatccg
     1261 ggtatgaaaa agtgaatgga gaatggcgta agaaatcacg gatgcctaca ggcggctaca
     1321 ccaacgaaca ggcaggctat tcatcaacga caaagcgttt taatgacgcc gataacaatc
     1381 caccggatac acatattcag gcgccggctt acgatgccac cgtcacttct cccgggaaaa
     1441 ccgataccgg gtccgggaag caaaatcccc ctgaaccgga aaaagccagg ccagcagcac
     1501 ctgattcgcc atctgcgatc ccggcaacag cactggcaga ctgtacgctc acaaacggta
     1561 aggctctgtc tgctcttgca caggatggtt ttccctatac ctaccacctt ctcaaaaaag
     1621 acggcacgct ggagctggaa ttaaaagaag gcatgtttgg cgtcagtgcc tttcattact
     1681 ggcgcgatct taaccggggt aaaggaaaac tgaaatatct gcgttttcat aaaggttcat
     1741 acgactatgt ggttgtgcag cgctacgacg gagaaacacc ggcatttgat ggcattctcg
     1801 tgtttaacca gggaacccgg attgccaccc tgaactgcag aaatactttc tctgaattgt
     1861 tcgccattga gcagttcccc gacaacagcc acaaagacaa cgaggatatg gctgatttta
     1921 ttgaaggtta cactgtgaga ggagaagcag tccgctaaaa acaacacaat attttatttc
     1981 tgaaaattcc acatacgtaa ttaatgataa ggaaaccgtg atgaatgatt ccactatttc
     2041 atcgttaaaa gaaaaactga atacatccac atggcatctg gtattactgg gagccgccac
     2101 atatggtgtt tatccactga tgtggctgta taaatatcag gacaccatca tggatgaaac
     2161 ccgtcagcga ttctcctctt ctgcaatgat tatctggatg gcagtatgcc tgggaatatc
     2221 cgccatgctg aaaatggctt ttccggttca gatcgacgaa tacggtgacg tctatggtta
     2281 cagtcgcggt gctgaaacca tgtacggtat tgccacgctg gtctcactgg tctggattat
     2341 cctcaccatc atctgggctt tcagagccag agcggctctg caacaatatg cactgacaca
     2401 gttccgcttt gaattaaaaa tgaacccagt atggacattt ttatttcaca ttttctacat
     2461 caattattgt attaatgcca tgccggaatc gctggcaaaa caccggataa tttatggtga
     2521 acctgactcc cgcccccggg ataactcacc tcagtaatat atcctttgca tttatttcat
     2581 tactggcgcc accgactggt ggcgtttttc tctgatttct tctgcagagc atatgttctg
     2641 tttactatcc gaaggccctt tctctcgaga gcaggcggtt gccatcacaa cagcttatcg
     2701 caatgtgttt atcgaaaatg accagggaac gcatttccgg ctggttatcc acaatgctga
     2761 atgtcagttg cgctggcggt gctggaattt tgaatacgat gccggcaaac aacttaatgc
     2821 gtggctcgcc agtgaagggc ttcccagaca ataaacctca ttcatttcat ccatcaggcc
     2881 gcgtcttctc cgggagacgc ggctttttca tttataccgc taactcattc ataaggagta
     2941 aataatgcga ttagcaagtc gttttggtca tgtaaaccag atacgccgtg accgtccgct
     3001 gacacatgaa gaactgatgc actatgtacc cagtattttt ggggaagatc ggcatacctc
     3061 ccgcagtgaa cgctatgcgt acattcccac catcaccgta ctggaaagcc tgcagcggga
     3121 aggttttcag ccattcttcg cctgccagac ccgcgtgcgc gacccgggcc gccgggggta
     3181 taccaaacac atgctgcgtc tgcgccgtga cggggagata aacgggcaac atgtccctga
     3241 aatcatcctg ctgaactccc atgacggtac ctccagctac cagatgctac cgggttactt
     3301 caggttcgtc tgccagaacg gctgcgtctg tggtcagtct ctgggggaag tgcgtgttcc
     3361 gcaccgggga gacgtggtgg agaaagttat tgaaggggct tacgaagtgg tgggcgtttt
     3421 tgaccggatt gaggaaaagc gtgatgccat gcagtcgctg gtcctgccgc caccggcacg
     3481 ccaggcgctg gcacaggcgg cgctgactta ccgttatggt gatgaacatc agcccgtcac
     3541 cactaccgac attctgacgc cacgacgccg ggaggattac ggtaaggacc tgtggagtgc
     3601 ttatcagacc atccaggaaa atatgctgaa aggcgggatt tccggtcgca gcgctaaagg
     3661 aaaacgtatc cacacccgtg ccattcacag catcgacacg gacattaagc tcaatcgcgc
     3721 attgtgggtg atggcagaaa cgctgctgga gagcatgcgc tgatgctgtt tccctgaaag
     3781 tgcagccctg actcacgtct gtaccagtcc ccaggcattc ttccattcat ttacttttta
     3841 taaggaatca tctcatgaca accttttcgc atagctccac cacaccttct gtttccgtaa
     3901 ccactgcatc agggaataac cagtctcaac tggttgccac catagtccct gatgaacagc
     3961 gcatcagctt ctggccgcag cactttggcc tcattccaca gtgggtgacc ctggagcccc
     4021 gtgtctttgg ctggatggac cgtctgtgcg aagactactg cggtggtatc tggaatctgt
     4081 acaccctgaa caatggcggg gcatttatgg cacccgaacc ggatgacgat gatggcgaaa
     4141 actgggtact gttcaatgct atgaacggta accgcgctga aatgagcccg gaagccgccg
     4201 gcattgccgc ctgtctgatg gcgtacagcc atcacgcctg tcgtacagag aattatgcca
     4261 tgacggttca ttattaccgg ctgcgggatt atgccctgca gcatccggaa tgcagcgcca
     4321 ttatgcgcat catcgactga atggaggcag ggacaatgca gcagctttcc tttctgcccg
     4381 gagagatgac acccggcgag cgcagcctca ttcagcgggc cctgaaagcc ctggaccgcc
     4441 atcttcatga acccggcgtg gccttcacct ccacgcatgc ggcacgggaa tggctgattc
     4501 tgaacatggc gggactggag cgtgaagaat tccgggtgct gtatctgaat aaccagaatc
     4561 agctgattac cggtgaaacc ctcttcactg gcaccatcaa ccgcacagaa gtccatcccc
     4621 gggaggtgat taaacgcgcc ctgtaccaca atgccgctgc cgtggtgctg gcgcacaatc
     4681 acccgtccgg tgaagttatc cccagcaagg cagaccgcct tatcaccgag cgtctggtac
     4741 aggcactggc cctggtggat atccgggtac cggaccatct gataatcggt ggcagccagg
     4801 ttttctcctt tgcggaacac ggtctgcttt aacccgtcac cgtcacaatc accttcatat
     4861 cacttcagtt tctctttctc agctgtttct tactttcaca ttcaggagga ctattctcat
     4921 gaaaatcatc acccgtggtg aagccatgcg tattcaccgt cagcatcctg catcccgtct
     4981 ttttccgttc tgtaccggta aataccgctg acacggtagc acggatacat ataccggccg
     5041 tgaagtacag gatattcccg gtgtgctggc tgtgtttgca gaacgccgga aggacagttt
     5101 tggtccgtat gtccggctga tgagcgtcac cctgaactga gtctggaagg gcattcagaa
     5161 gagcagaatt atcgccacca ccggaccatt cttaaccaat tttctgtgag gattttatcg
     5221 tgtcagacac actccccggg acaacgcttc ccgacgacaa taacgaccgc acctggtggg
     5281 gactgccctg cactgtgacg ccctgttttg gtgcccgtct ggtacaggaa ggcaaccggt
     5341 tgcattacct tgccgaccgc gcctgtatta gagggcggtt cagcgacgcg gattcgtacc
     5401 acctggacca ggcctttccg ctgctgatga aacaactgga actcatgctc accagcggtg
     5461 aactgaatcc ccgccatcag cataccgtca cgctgtatgc aaaagggctg acctgcgaag
     5521 ccgacaccct cggcagttgt ggctacgttt atatggctgt ttatccgacg cccgaaacga
     5581 aaaagtaact ctccagaata accttctgct ccggcctggt gctttcacca cgccactgtc
     5641 ccatttttta tctctgcata tcaggaaaat cttcattatg aaaacattac ccgacacgca
     5701 tgtacgggag gcatcgtgct gcccgtctcc cgtcaccatc tggcagacac tgctcacccg
     5761 gttactggac cagcactacg gcctcgcgct gaatgacaca ccgttcgctg atgaacgtgt
     5821 gattgagcag catattgaag cgggcatttc actgtgtgat gcggtgaact ttctcgttga
     5881 aaaatacgcg ctggtacgta ccgaccagcc gggattcagc gcctgtaccc gttctcagtt
     5941 aataaacagt attgatatcc tccgggcacg ccgggcaacc ggcctgatga cacgcgacaa
     6001 ctacagaacg gtaaataaca ttaccctggg taaacatccg ggggcgaaac aatgaaactg
     6061 gccctgacgc tggaagccga cagcattaat gcgcaggcgc tgaacatggg gcgcattgtc
     6121 gttgacgtcg atggtattaa gctcgctgaa ctgattaaca aagtcgctga aaacggttat
     6181 tcactccgcg tggtggatga acatgacgct accgaaacac cggcgacttc tgccagccct
     6241 catcagttgc cggaagacaa tacagtgata cagagcgtga tgccattaac cactgaaaca
     6301 gcgctggata tcctcattgc gtggctgcag gacaatattg actgcgaatc ggaaattatc
     6361 tttgacaacg atggggacaa aacggattcg gcagcactgt tgccctgtat tgaacaggcc
     6421 agggaggata tccgtaccct gcgccaactg cagcttctgt accagaaccg gtgagtctta
     6481 ctcaccagac ttcattccac tgacgccagc ctgaacaggg caggcgtttt catttatctg
     6541 caaaaaggaa tatcgattat gtctgaaatc acagcccccc gtccggaagt ggtcaacggg
     6601 catacggacg ttatctgctc aaccagtatc cgccacattc tggctgtacg aaagagtaca
     6661 ctgctgcaaa tcgacacact tatccggcaa ctggctgaaa tatcagcaat gacagaaagt
     6721 attggaggta aaaccgcccg ggactgggcc atgaaacagg attttcgctg cggttgctgg
     6781 ctgatggaga aaccggaaac cgcaatgaaa gccatcaccc ggaatctcga tcgcgaactc
     6841 tggcgtgacc tgatgcaacg ttcagggatg ctgtcattaa tggatgcaca agcccgtgat
     6901 acatggtacc ggtcactgga gtacgataat tttccggaaa tctgcgaagc gaacattctg
     6961 agcacatttg aacaactgca ccagaataag gatgaggtgt ttgagcgagg ggtgatcaac
     7021 gtcttcaggg gactgagctg gaattacaaa accaattgcc cctgcaaatt tggcagtaaa
     7081 attatcgtca acaacctggt gaggtgggac cggtgggggt ttcatctgaa caacggccca
     7141 caggcagatc gacttgccga tctggaaaga atgctacacc tgttcagcgg caaaccgatc
     7201 cccgacaacc gggaaaacat caccattcat ctggatgatc acatccagtc tgttcagggt
     7261 aaagaggact atgaagatga gatgttcagc atcagatact ttaagaaggg ctccgcgcac
     7321 atcacgttca ggaaaccaga gctggttgac aggcttaatg atattattgc cagacactac
     7381 ccggaagccc tggcaagcac agtgaacaaa agcagcaagg cataaaagag gtattttaca
     7441 attcacgaat attaactatt attaattgat agtaatcaat cggcgagaaa ataaccatgc
     7501 ctaccagtgt atccctcagt ccttactttg aaacgtttat ccgcgaacaa atagaaagtg
     7561 gtcggtacaa caatacaagt gaagtcattc gtgccggact tcgtgctctg gaagaacggg
     7621 aacagcagat aaagcttgaa tcactccaga gcgccgtgac cgcaggcatc aacagcggtg
     7681 aaagtaaaag tgctgaagag gtattcgggc gactgacgca caaatacaaa aagatggcag
     7741 aaggtgaaca gccaatatga aactgactgt ttcaccatta gcagaacacg atcttgaagc
     7801 tataggtgac tggatcgctc aggataatcc tgtccgtgca atcagtttta ccgaagagct
     7861 ttaccaacaa tgtctgttga tagccgaatc gccagtactc tacagagaaa gacccgagtt
     7921 aggggctggg atcagaggtt gttcgtatgg tcgttacctt ctgctgttca gggtactgga
     7981 tactgaagtt cgtatcgaac gtatcgttca tggttcccgg gatatcaaca gggtacttgc
     8041 agagccagac gacaaaccat cagatcattt ctggcagcca taaacaaaat ctgtgcgtta
     8101 acgctcccta acacaggtag ccaggtattt tataaatctc tgtattttgt tgtcacttcg
     8161 attccgcgat tcagcatatc gaaccagaca aaatgattac aacaaaccac aagaaagagg
     8221 gattatagcg tcaaaagaat tcgaattgca catgggggcc tcattggtgg cctcaaaaat
     8281 aacaaacaca aaacaatcaa tataaaacaa cttgttatac aaccagtttc attccgagtc
     8341 cggcaccact aattcttaag aacccgccca caaggcgggt ttttgctttt ggatctgaca
     8401 ataaccttca cgaaaaaaat tagcttataa agtctggggg aattactctc gccacgttaa
     8461 cgagagtaat tttattgata ttaatctcct gatactttac ccccgtccaa actccagccg
     8521 ctgcacattc accatcccag gcttctcagc agcactgaca tcaatttgtg tcacccgcag
     8581 cgcatatttt tcatccagtg cgtttaacca tttcagcagg tcattaaaca ccacaggttc
     8641 tatccagacc tgaatattct ccccacgctc ggctatccgc ctgatgacta ccgagtgcgc
     8701 ggaagcactg tcactgataa cccgcgatac ctgcgcaggc gttgttgtgg cggattttcg
     8761 cgccgcaata atatccggcg cggcgctctt cagtcgggtg ttcatcgcca ccagctgctg
     8821 caatattgtc tcctgttgct caatccgttc gctcaaaggc tgccagatga ggacgtaata
     8881 tccgacgcta aacaggaaca ccaccgctgc cagtaacatg cccttttcac gcggagaacg
     8941 ccccgccagg tgttgtgcca gccagtgttc gccacggctt aactggcgtt cacgccattg
     9001 ctgaaaatag tgaataaatt tatcgcgtaa catgttattt cctccgcaac gttacgccgc
     9061 cggaaaccgc atcaccctct ttctgtaacg cgtcctgctg cacaacaaaa tctgccgcca
     9121 gtgcgctacg cagtttgtcg aagctggcaa agttcgcggc ccgcaactgg aggtgaagcg
     9181 tctggcgttt ttgatcaaag gtgaaaccac gcatttcgat gtcggaaagt gacgctgatt
     9241 tcagggtgct ggctatcgct gacaactcgg cgagcagccg ggtatcgtcg gcctgtgggc
     9301 gatatttttt cagcgccatc gtcacctgag agcgtaaatt cacaatccgc ttctgctccg
     9361 ggaacaacgt taagaactgt tcctccgcct gggtgcggct ttgcgccacc tgttcgctga
     9421 cgctccataa cgtcacgccc cgctccactg ccagcgcaac cagaatcagc aatatcggca
     9481 gaatcatcac ccgccagcgc gcccactgtt ttcggtagct gacgcgaggc tgccacggcc
     9541 ctgtcagcag gttcccttcc ggttcgccat aagtggtaac ggcgggcaga gctgtaacgg
     9601 tcaggcgttc agcgtctgcg ccagcccatg ctgatagctt ttccggtgca atgccgacta
     9661 cggttagcga aagcggtaaa tcctgctcat tgagctgggc gcggaacatg accggagcca
     9721 gcgccagccc ggcgctccat ccccggcatt catcgatgcg gcagataacc cgttgcgcat
     9781 cgccagccat aaacccacaa ggaatggaca tccagtccgg cgcgacggta gcgcgggtga
     9841 tgccgttttc ctgcaaccac tgcgcaatgt tgcgcatatg ctgttggtga atcactgcta
     9901 cggttgccag ttgctggtcg attttcaacg gggcgaaatg cagttcatcg atatcctggt
     9961 tcagctcttc ttccagtaag gcgggcagaa tagtcggtat ctgcttgcgg gcgacaacag
    10021 gcagttcaac ctgccagacg ctgatccatt cgccgggaat gtagagtcga atcgcatcag
    10081 tttgcagcca ttgctggagc tgttcgttag caacgtcaga ccagataccg tgctccacat
    10141 cgggtgtacg gcgctgccaa tggatgggat cagcggcgca aagcgggaaa aaaatctcaa
    10201 ggatggaact cactcacttt ctcctgtctg atgccagaga accgaaaagt gttgtgggcc
    10261 catgcggaca attaacgagt tcatcgtcag ttcaatctca ttcacggtga tatctgaacg
    10321 cagccagaag taattgctgt ccacgctcag gacggttttt agctgttttt tagtacgctc
    10381 atcgacgtca gcaagtagcg gctgtgcaag aaactgatcg acatcttccc agcccttcgc
    10441 cggacgttgt tgtaataacg cccgcgcctg aacagggctt aaccacgggt caaacagcgc
    10501 ctcaagaatc acactttgcg tgacgtctaa agtattgatg ttgatttgct ggcgggtcat
    10561 cggcagcgca cagaccagcg gtttcagttt ttgataaagc ccggcgtcca ttccctgcac
    10621 cacgcgcatc tcgctgatat cggccagcgg ttgattggcg gcgtagaaag gcaccgagcg
    10681 ggcgagatat tcgctgtctt cacggcccag acgcgtctgc acgctgcggt cttcatcaat
    10741 aaactcccac aggctttcgg ctatcagttc ggcccgataa gcaggcacat ccaggcgcgt
    10801 gatcagggca atcagttgtt gtaccgcgag cggacgcgac gccgttgtgg gctgagcgag
    10861 ggcattcagg ttaaagcagg cctgtgcgtc acgcagagtg acggcgattt gcccctgcgg
    10921 cagtgggaaa aaacgagggc cggaagccca cggttgtgcc agatgcacgc gcttttcatt
    10981 tttcaggctc agactgagcg cgctcaacgc caggctttct gcactggcgc tgtaccacag
    11041 cgcctgctgg tactcctgct ggctgcgcgt tcttcccagt tgctgctgca tccgcccgga
    11101 aagcgtgatg gttaccagca tcataactgc cagcaatacc agcaccacaa ccagtgccat
    11161 tccgcgtttt ggtggtgagg tgatcatgat tattgcggcc cgcgtaacaa ccagatgcgt
    11221 tcaatctcgc cccattgcgg cgaatgcagg gtcatgcgta ccgccacagg gatcgcctgt
    11281 actgacgacc agctctcctg ccagcgcgtg ccgtcgtaga actgcaaacg gagcgaatcg
    11341 gccggaatca acttttgcat tgtcggcttc acgctgcctg cggcatcggt cagcggccag
    11401 gctaaccgtt cgagataacc gtcatgaatg cggtaaccga cggtgagcag attactgcgc
    11461 ggcagacgca tcaacgggtt aaccacaccg ccgcgcacaa aacgcattcc ttcactctca
    11521 gacgccagca cgccagcgcc agccagtaac gctggttcac gctggccctg atcgcctctt
    11581 accggacgcg gcatcatttg ggtcagatcg tgggtcagaa aactcatcgt ttgctgcatg
    11641 aggtttagtt tttgatcgtg tccggcgacg gcgctattca cgcgcgtgac gccgttagtc
    11701 acctgctgcg ccatcagtgc cagcgaggca aaaatggcaa ttgccaccaa catttccagt
    11761 aacgtgaaac cagcgcgagt ccttctcact gttggcctcc cacggcgcta aaccaggcgc
    11821 gtcgtgactg aatcaccgac gaaaagtctt cgtgaaggct gacttcaata tccaccgcct
    11881 ggagcagcgc gttgcctgta ctcagcggtg tggttcgcca gtaccagcgg cttcctgcca
    11941 tatcgctctc gccctgctgc ctgaccgccc gctgctcgcg ggtcatcagg tagagcagcg
    12001 cggtctggtt ttcagccacc cagcccgcta ccgttttttc ctcaagaatt tgctgattac
    12061 tcagcgcacc gctggcaatc tgtaacaccg ccgtggcagc cagcgcaaaa atcgccagcg
    12121 cgagcatcac ttccagcaag gtaaatccgc gcttcatggc atcctctcat cacattgatt
    12181 gagggataac gcgccatcgt gcgccagttt taccgcccaa catgcgtttt gcgccgccga
    12241 gtagaagcgc agcgtaaacg gcgtggcggg ttcaaaaggc gaaaaacgga tctgcggcgt
    12301 cttctttttc gcctcttttt gcagctccag ttcaatatcg tgcagcgtca ggcgacgacg
    12361 ttgcagctcc agcgcatact ccttctgcca gatatcactt ccgggttgta acagcatctg
    12421 cacctgtttt ggcaccgtaa cctgtgccga taaccgggtc gcggaaacgg gcaaccactg
    12481 cccctgacga cgctgcataa actgatagcc aggcgcgtcg attagcacac cgagtgtctg
    12541 cccttcaatc actgccctgt ccttaaactg cgcaaagcga gtcagaaaat cctgtgccgc
    12601 ttttttcgcg ggcggttcag aatcggtcgc aaacgtctgt accacgcccg aactggcaag
    12661 gccgataagg aaaatcacca acatgatttc cagaagtgtg aatccgcgtt caggcactgc
    12721 ttactgaaac tcctgcaaat tccagttacc gatatctgcg ccagcgcctt ccccgttttc
    12781 ctgaccatcc gcccccaggg tataaacatc aaacagtcct ttttcacccg ggctgagata
    12841 ctgataatca ttgccccacg gatcctttgg cagtcgtttg atgtatccac cagtacggta
    12901 gttgcgggag tccgccatat tggctggctg ctggatcagc gcctcaagcc cctgttctgt
    12961 ggtcggataa cgcccgttat ccagtcggta catatccagc gcattctcca gcgccacgat
    13021 atcgctgatg gctttttgcc gatccgcttt ctctttattg cccaacaggt taggcaccac
    13081 cagacttgcc aggacgccaa gaataacaat caccaccatc acttccagca gggtaaaacc
    13141 tgcccgtggt ttttgtgtgc gggataacga atacatgtga taactccgta aattacattc
    13201 caaccatatt gttcagttgc agcatcggct caaggatggc gatgacgata aacagcacca
    13261 cgcccgccat catcaccacc agcgccggtt caaacagccc taacgccagc cccacctggg
    13321 tatcaaattc ccgttcctgg ttgactgcgg cctgctcaag catggtttcc agttcgccgc
    13381 tctgttcgcc ggaggcgatc atgtacagca tcatcggcgg gaacaggcgc aactccgcca
    13441 gcgcggcgcg cagactgctt ccttcgcgga cgcgatctgc cgccagcagt agttgttgtt
    13501 cgacatagcg atttgccgac acggcggcag cggtctgaat accttccagt agcggaacac
    13561 tgctggcggt gaggatgctt aaggtgcggg aaaaacgcgc cgtattcagc ccgcgcgcaa
    13621 cacgccccgt cacaggcagg cgcagcacgg tttgatccca gcgcaggcgc atagcaggat
    13681 ttttaagtag ccgttgcccc agcaccagaa gccccagcaa acccgccagc cagtaaacgc
    13741 cgctggcctg taacgcgtcg ctcatggcaa taagcgtgcg ggtggaggcg ggcagcgcgt
    13801 gtccgagatg atcaaactgt tcgatgattt tcggcaccac tgccgtcagc aaaatagtga
    13861 ctacgcccgt tgccaccacc agcagaacca gcggatagag catggcctgc aacagacgtg
    13921 atttcaggcg ctgtcgctgt tcggtgtaat ctgccaggcg attgagcacc acgtcgagat
    13981 gcccggattt ttctccggca gcaaccatcg aacaaaacag ggaatcaaag acgcggggat
    14041 gttcgcgcag gctatccgac agggtgtaac cttcctgaat ccggctgcgc agcgccattc
    14101 cgaggctttt tacatgcagt ttttcacttt gctcacttac cgcctgtaag caggtttcca
    14161 gcggcattgc tgcctgtact aacgttgcca gttggcgcgt gaacagcgcc agatctgccg
    14221 ccgccacgcg acgatgtgcg tgccgccgac gctgcaacat cccccctgac gaagtattca
    14281 tccgggcttc aatatgcacg gggataagct ctttaccgcg caacaactgg cgggcatgac
    14341 gcgcggaatc cgcctcaatc atacctttgg ttttgcgacc attacgctcc agcgcctgat
    14401 agtaaaacag tgccattacg cctccatggt tacccgcaga acttcatcga gagaggtttc
    14461 tccggcgagc actttctcaa tgccgttgct gcggataccc gcagagtgtt gtcggacata
    14521 acgttccagc tccagctccc cggcctgacg gtggatcaaa tcacgcaatg tggcatccac
    14581 cacgatcagc tcatggatgg cagtccgtcc gcgaaaacct ttgtgattac aggcgggaca
    14641 gccctgtgga tggtacagag tgacggtacg ggcgtcggta attcccagca ggcgtttttc
    14701 ttcgtcagtg gctggtgcgg gctggcggca gtcgggacac agcgtgcgaa ccagtcgctg
    14761 cgccatcacg cccgtcagac tggaagagag caggaaaggc tccacgccca tatcctgcaa
    14821 acgtgtgatc gcccccaccg ctgtgttggt atgcagcgtg gaaaggacca ggtgtccggt
    14881 aagtgaagcc tggacggcga tttctgcggt ttcggtatcg cggatttcac cgaccatcac
    14941 cacatccggg tcctgacgca aaatcgcgcg caggccacgg gcgaaggtca tgccgacgcg
    15001 ggtgttaacc tgcgtctgac cgatcccttc aatcatgtat tcgatgggat cttcaaccgt
    15061 gagaatgttg cgcgaatggt tgttcagctc ctgcaatccg gcgtacagcg tggtgctttt
    15121 gccggaaccc gtcggccccg tcaccagaaa gatgccgtgc ggtttgtgta acagctggcg
    15181 caactgcgcg gtcagttgct ggctaagccc cagacgctcc agcgtcaggc gggcctggtt
    15241 tttgtccagc agtcgcagca ccacgcgctc gccccaggca gaaggcatgg tggagacacg
    15301 tacgtcaatc gcccggccgc ccagcaacag cgcaatacgt ccatcctgcg gcacgcgctt
    15361 ttcggcaatg tccagccgcg ccatcacctt gatacgcgac accagcagcg aggccagttt
    15421 gcgccccgga cgcagcattt catgtaatgt gccgtcaaca cgaaaacgga tcaccagact
    15481 cttttcaaac gtctcaatgt ggatatccga agcgccttct ttgattgcct ctgccagcat
    15541 ggcgttgatc agtttgatga tcggcgcatc gtcgtcgctt tccagcagat cttccgtttc
    15601 tggcagttct tccgcgagag taaaaaagtc ctcagcagaa ccgagatctt ccatcagctg
    15661 gcgagcctcg gaagagtccc gctgccagac cgcattcagc cgctgttcaa attcggcctc
    15721 gtcgatttgc cgcagcgtaa agggcgcgtt cagcccccgc tgcagctcct gcaaaacaga
    15781 gagcgatagc ggatgaacgt ggaggatctc cagcgacgct tcgcaccatg ccaccaggct
    15841 aaaccgacgg ctgaaactgt agggcagacg cacggtgttg gcggtggttt cctgtgctac
    15901 aggcaccatt aacgcgttct cccggcattg aggaacgcgc gaacttccgg cggtaaggcc
    15961 tggttttgcg ctggcagtac cggctgtgcg gtgtgcggca tcaggcttaa accttgctca
    16021 tcacgataga tctgttcggc gcgcatatag ttatatttgc gctgcgacac gccgtctgcc
    16081 gccataccgt cacgcagaat ggtcgggcgg ataaacacca tcaggttacg tttttctttt
    16141 ttatccgccg tcgatttaaa caggttacca atcaatggga tatcgcccag cagcggcact
    16201 ttcgccacgc tttctcccgc ctgatcgtcc atcagaccac caagcacaat cagctcacca
    16261 tcgttagcca gcacggtggt tttcagtttg cgctcaccaa acaccacgtc gaggctggtc
    16321 tgtccttcca ccttcgagac ttcctgctca atcaccatct gtaccgcgtt accttcgtta
    16381 atctgcggcg tgactttcag catgatgccg acttttttcc tttctactgt attgaaagga
    16441 ttgctgttat tggagccaac ggtagagccg gttaataccg gaacgtcctg gcccaccatg
    16501 aagaaggctt cctggttgtc cagcgtggtg atgctcggcg tggagagcac gttcgagctg
    16561 gagtcgtttt taaccgcctg caccagcgcc atccagtcgc ctttcaccac accaaccgcc
    16621 gtaccgctaa agccggaaag aagctgggcg agcgtggaga gatcgccgtt ggtatccgga
    16681 ttaatggtgg tagcgccgtt ttcactgatc accgtcgagc ctttctgcgg tttcgcctga
    16741 gaaatggctg cccccagcgt accgataggg atctgcgtac cattagcaaa ctgcattaat
    16801 ccggcatctt tcgacgccca ctgcacgccg aagttgatat tgctaccttc ggcaacttcc
    16861 acgatcaacg cctcgacgtg cacctgagca cggcgaatat ccagttgttc aatcacgctt
    16921 tgcagcgact gcatgatgtc ctgcggtgcg gttacaatca gggcattact gtgtttgctg
    16981 gcggcgatgg atacaatctc acgcccgcta ccgaccgtgc cttccgcctc ttctttagcc
    17041 gccgtgagcg tgccgctgac ctgcttcagc acatcgacca gatcttcggc tttgctgtat
    17101 ttgagataga aaacctggct gttgccgctg cgttccattt ctgagtccag ccgacggatc
    17161 aggcggcgca ttttgtcccg cgtggccggg tcaccactga caatcacact gttggtgcgt
    17221 tcgtcggcga caatttgaga tttcagcgtc gctggctggt tctcgccgct gtttttcgtc
    17281 aggctttcca gcacgcgggc gatttccgaa gccgaggcgt tatccagcgg gatcacctct
    17341 tcggtgcgat tacccgcgtg atccacgcgc tggatcactt ctgtcaggcg ctccacaacg
    17401 gaggcgcgtc cggtgagcat aatcacgttg gagggatcgt aattaacaac gttgcctgag
    17461 cctgcgctgt cgatcatctg gcgcagaatc ggtgccaact cgcgtaccga cacattacgt
    17521 accggcacta ctttggtgac catttcatcg cccgcgtagt tgtcgctgcc ttcaccgacc
    17581 agcggcagcg gctcgacttt cgcagcgctg gatttcacca ccttcagcac gtcgttttcc
    17641 atcggtacga cggcataccc ctgtgcttcc agcaggttaa ggaatagctg gtaatactgg
    17701 cgttcattga gcggggtcat ggtgcgaata ctcactttcc cctgtacgcc cggccccata
    17761 atgatggttt tattaaggtt agcgccgacg gtttcgatga acgatttcag gtcggtatct
    17821 ttaaaattag cggtgaaagt ggcttcttcc gcccagaccg gtgaactgca taatgccgct
    17881 gccagcacca gcggcagtaa acgctttttt gttttgaggc cagttgtctt cttacgccag
    17941 accgacaacg taatatcacg ccaaaacacg atgaatgatt ctcctggatt aaatgcggtt
    18001 agcgcagcgc gatggaaatg tcgtggcgcg cacccttgcg taaaaccgta agttgaatgg
    18061 aatccattga aggtaactgc cgcatcagag caatcattgc tcgtggatca gtgaaatcct
    18121 gctgatttag cgcaatggcg atatcgcctt ccttgaaacc gctggcatcg aacagagaac
    18181 gatctgcccc cggcttcact gcataaccga caattccctc cttacgcaca ggcgtaagct
    18241 ggatatagtt aaaaattttc tgcggatctt tcgccagtgc ctgacgcacg gcagtcggga
    18301 tctccactgg cgcactgaca gcaggttcag caacagcttg ctttgcttcg tcactgacag
    18361 cttttttgtt ggtcacggct acggtggaat gcccctcttc tgccaggctc aggcgctcta
    18421 tttttccctg atagcgcagc atcacatggt cgcgattgat ttcctcaatc accgcattgt
    18481 gcgagccaag cgtttcaccc tgcaaataga cctgctgttt accgccttct tcaataaccg
    18541 cgccgggtct ggcaccaaag gcaatcccgc gcagcaccac attaagacgc gtttctgcca
    18601 caggcacagg ttcgggttgt tttaccggcg tggcgacggg ctgatatttg ccaaaccagt
    18661 tttgctggct gattaattgc acgtcatttt tatcgaacgt ttttgcatcc gcacggggcg
    18721 gtttattcgc cgatggggaa acagccgtat attccgcaga aaaggagaaa tagcgccaga
    18781 gtgaatgcgc cacttttgca gaaataataa gcagcatcag ccagaacatc ccgcgtacaa
    18841 tttttcgcag atgctctttg tctgtattaa aagattgtct ctggttaaga gtgtgacgaa
    18901 tttttgtcag ccattgaatg agataaattc gtgcgtcacg aaaaacaacc cgcgccaatt
    18961 tatatctacc cgacgttatg ctttgactat tccacaggtg gtacgatcca gtttccgctg
    19021 atactggtta cctgtacgtg tatcattaat cgttatgctg taattaattc cctcggtaat
    19081 cattaatttc accgttgggt cagcgcacat ttgtcgttga tagctggtta aaaaggcgtc
    19141 aggcgtctgc gtggtctgag tccccgcttc gctgataatg gtcattttta ccgtcgtgcc
    19201 gctactttgt gccagcacta aggtatatcc cgcagattta atcggcaggt tttggctgat
    19261 attttgcgcc tgttttttcg ccagtaaact ggcattttca ttatggctgg cacagccact
    19321 taataatcct gtaacgctca acaatagcga tattaatacc ctccctggca tttgttttat
    19381 cgacatggta aataatctct aaggttatta ataagagtta aaatgtcact ttgataatga
    19441 cgttgttatc attaaaacaa tgcctgtaga taaattgttg ctataccgcc cagacttaaa
    19501 cacggtccaa aaggcagtgt ggttgatcct ctttttgtaa taacggcata tatcaggccg
    19561 cagcatgatg cgattaaagc aacattgggt agcgacaacg gccccaccca actacctaac
    19621 gcagcgaaca ataatacatc gcccatgcct aatgcttctt tacgcagaac tactccggct
    19681 atccagcgca gggagtaaaa agcgataaac cccaccagga cgccggtaac tgcatcttgt
    19741 agcgtgagcg ggctctgctg cgcccatgcc gcaatcagtc ccgtccacaa tacgccctga
    19801 gtaaaaacat cgggcagcca ttggtgatcg aggtcaatga cgctcgcggc aatcagccag
    19861 gcggataata tcatcaccgc cagcgcccat ccactttccg gccagaccag actcgccagc
    19921 aaaaaagcga gtgccgtcaa taactccacc agcggataac gcttgctgat tttcgcctga
    19981 caatcacggc agcgcccttt gagcatcagc caggagagca gcggaatatt gtcacgtatc
    20041 cggatggtct gctgacaatg tgggcagtgg gaacgcggta gcgcaaggct tatttttgac
    20101 tgcgcactcg gcatttcacc atgaaactcc gccatttgtt ggcgcagcat gatggggtaa
    20161 cgccaaatca ccacattcaa aaaactgccg atgatcaatc ctccgacggt tgccagtatg
    20221 ggcatcgccg cggggtattg ctgaaaaaca tcaaaaagca tggttaaagg ttgtttgttg
    20281 taacttgccg gatgcgggcc tgcaagtgta tgccatacgg ctttcctcca ggcccgataa
    20341 gctggcgcaa cccggtgcgc cttatttcat gccggatgcg gcgtgaacgc cttatccggc
    20401 atacaggatt actcgacaga catcttatgc tcggtaacct tgttaatggt ttccggtccc
    20461 tgttccggtt tcggcagctt gagtgacgcc agcgtgttgt acgccgactg gctcacaccg
    20521 ccctcgaagc tcatctcgct cgcccccggc agctggtaag cattcgcacc cggattccat
    20581 ttcttaaaga actccgaaag atccgtctgg gcgacccagg aggcacacag catcagcgtg
    20641 tccgcagcgt taccgttgga ttcagcacag taattcttgc caccaaaggt cttgtcacca
    20701 acgtcatcgc cgcgcgcttt acggtgcatc aactggaaca ggttccagcc tttcatccct
    20761 tcacgatcgc tgaagaactt cggcaattca ccttctggat accatttctt gatatcaaag
    20821 tttttctctg cccattcctt cagctgcgcg tacatcagca gacggtcacc cgcaccgccg
    20881 cgcgcccatg cctgaccgtt gctctcctcc agatattccg gtgcgacggt aatatcgtca
    20941 gcgacacggt tcatcttgcc gagataacga tcctgcatgt acagcgccag cacgttgttc
    21001 gccacttcgg tcgcgcctgg aacagtcagc ggcgtttctg cggcgttgtg ccctacttcg
    21061 tgccagatca gccagtcgtt cagcggcgtc gtcggcagcg tggtgctgtt cgtcgagaag
    21121 ctgctgttca ttaccggata acccgagtgc gcatcaccga tggagatctg cacatcgttt
    21181 gtgaaacgat gtttatgtcc cgtcaagttt ttataggtaa acatccggtg cttaccgctc
    21241 gtttcatcac gaccgtagaa gtcattcatc gagctggcaa aggtgtccag atcgttagcg
    21301 aattgctcca gtccgccagt gtaattgctg gcattcaggt tcttcttcgg tgtggtatag
    21361 acgaaagcgt ctgattccag ctcacccagc ggagccggtg agttcagatc gtttttccat
    21421 gcgccgtctt tatagaacgg tgcttttacc acgccagtaa aggtgaagct ggcagattca
    21481 ttggtagagc tattgccctt gatataaatc aggccaccgt aaggcacctt gaacttcacc
    21541 gtaccgctag cgtccagaga gtacgtttta gtcactcttg gcggacggtt cagcgcaact
    21601 tcatgcttct cacgtccggt caggtcgtca gccagcgcca cggtgacgct cacaggaacg
    21661 ttcgcattgg acttaatggt gacctctttc tgagccggtg cccacaggcc agttgactgc
    21721 atgttacctg caaaccattt ggtcggattc gagtacaggc tgatggtttc agtaacgttc
    21781 tgtccctctt ccgatactgc tcccggatac ttctcgacat caactttgat gttcagatcc
    21841 caccaggaac ggcccagcat caggcgtgtc agcggttttt ccatatagtt gagcgggtag
    21901 ctcgggttca tcatgcccgc tttgctgcta ccgtcaccgt agatcatgtt gttatcgacc
    21961 agcgattttt tcagatctgc agaacacttg gtgccgcctg cataggcatc attggcgtag
    22021 cagttcagga actcggtgaa cgttttaaag cccagctcgt catttttgcc ttcttcataa
    22081 cgatagctcg tatcgttcca cagccagacc gacatgttct ggtacagacg ttccagatcg
    22141 acgctgctca gacgctcacc tttgcgacca ttggtccgga agtagagctc atgctgatac
    22201 agacgctgaa tgttggtgcc taaatccgca gcctgcacca tcgcttttgc agtgtcggcg
    22261 ttaaggctta gttgcgtata ctgtggaaca tacatgccac cagtaaccgg aacccccgtg
    22321 ccaggacgat attccaggca gttgacctca tagtgatatg ccggattagt acactctttc
    22381 agccccggga acgcggcgaa aattttctcc ttcgcagcct tcagagaatc ctctgtttta
    22441 tgatcggcct catcaataaa ggcataacgc gtttcctgtt tgccatctac atcttccagc
    22501 cagctggcaa cttccagctt cggtttgtca tcaggtttgt tttctacctg atatttccac
    22561 taaacttccc ctgtcttact atcgatggtg tacggcagcg caccatctac ggcaggataa
    22621 cgttcataga cccaaatgcc cgttgcgcgc tgctgacgaa cgcggttcgg atacccttgc
    22681 ggatcgttat ttactaccga cttgttcagt gccatcgaca gacctgcggc atccaacaga
    22741 cgcacaaaac cagacgcgct ctcttcctta agattgctca tcacgttttc catgatcagc
    22801 accgatccac ctttgttcag ataggcgatc agatcggtca catcctgctg agtcagcttc
    22861 ggtttgctgg tatctgcacg cagcgggatt gcataaggat cgttacccac ctgagtcaca
    22921 tattcaaagc cgttaaggat cagcagcggc atttcctgcg gatcgagatc gccatagcta
    22981 cttaaatgct caacagagat gcccgcaaaa tccggatgga agtcgaacgc agcgctgttt
    23041 cctgtaacct gaccatgacg tttaaaatag acagtatcca ggttggtgcc tacggtcatg
    23101 ctggctttcg cgtccggcgt ccatttatcg tcggacagat agcgcagcac gttctccatg
    23161 aagttcttca tgtcatccgg gtcgctgttg agcgtacact gcccatcttt attaacgccc
    23221 ccgttccagc tgtaaccgtt cgggcaacgc aaaatgctgt tgtagtgtgg gttaccgata
    23281 accatcagtt tgccctcacc gacttgcccc agcgaaataa acggcaggtt gaaagtggcg
    23341 gtatcgcgcg taacgttttc cggctcaaca agagaaggcg cttccgtaat gtacgccagc
    23401 tcatttttat cccaggcgcg tttttcgcca aacgccagca agtagttttt atcattacgc
    23461 gccatcagaa tcgggaaggc cgcgttggag atattcacca ccgcctgacc gcgcgcatta
    23521 cccgtgctgc catagaagtt ggtagagtca tggaatacat ggaacttact gacagatttg
    23581 tagttcgtat ccacgcccca cagcttgttg ataacgccct gaatctggcc gtcattaaca
    23641 ttgcgcgttg tcagcgagaa ccagcgagcc tcgttacaac cgtcggtttt cgcacaaatc
    23701 gcggtatcga tctctttggc ctgacccgtc ttaaactgct cgataaattc gtttggcaga
    23761 ttaacaactt gctcaccttc atccagcgtc gcaccgttgg ataacgagag attgataatc
    23821 tcgttgatca cgttgggata ttcggcaaag accttgcgta catcatccgg aacaacacgg
    23881 gtattatttt gcccggtcgt cgaatagcga tgaataagct gatcgatatt cgccccgcga
    23941 acttcatcac ccagttcagt cagcgcaatg gtcgacttat tgccgcgcac tgaacccagt
    24001 tcaaaggtat cgataccaaa ggagatggtt tcgccccagc taaaagaaaa ttcaccattt
    24061 tcccctgtca cgccacggcc tgaattggtg tagtagttga cgccagcaac accatatccc
    24121 tggctatcca ccaggcgacc ttctgagaga atgatttccg aaggttgata ctgataaaac
    24181 tgttccgcgt tagccgacac gaaggaggcg ttcagatccg gttttgttcc cggagtcgtg
    24241 acggggacta ccggtgaagt atgagtggat ggcgctttat cagtcgcggc attgttttcc
    24301 acctcttcat tgaccagctt tttgaactct tccggtgcca gatcgatttg cttatacagc
    24361 gagtcgaaac gtttactctc gatcaccgag gagaactcca ggcacacctg ttctgtattc
    24421 gccgggcaac tgttcatgga ggtgaccagc gagagcgcat tgcttttctt gttgtcggaa
    24481 cccgccagtt cttgcgcgtc ctcaagacta aacgacactt tttcaaccgc acgcaggcta
    24541 cgcgcagctt ctgactgggt gtcgaaggta gcaattgtcg tgttgttccc tgccacacag
    24601 gtgactttgt cgcctggtgt aaaggtaaag ccatcgctgg attcatcatt acaggtgata
    24661 tcaccagtta cccgcaggct tccgcccagg gtcagataac ccgttttcgt aggaacaggt
    24721 tctggctccg gttctggtgt tggctcagga tcaggtgtcg gttccggcgt aggttctggg
    24781 tccggcgttg gctcaggcgt cggctccggg tttggtgttg gatcaggttt cacttccggc
    24841 aaagaccctg ttccagaatc tacaggcggc gtatcggagg aagatccgga gccaccgcca
    24901 tcacaaccgg ctaacagggt tgcactcaaa atagccgcta aaagcgattt cttatattta
    24961 aatttcttat tcattaataa cgcaagtgac aaaacaatgt taaaaaattc gtaactggga
//
LOCUS       DAJOWB010000062        24972 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJOWB010000062 DAJOWB010000000
VERSION     DAJOWB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24972
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.062"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..749
                     /locus_tag="OHO85_002739"
     CDS             <1..749
                     /locus_tag="OHO85_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCQ2672091.1"
                     /translation="FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDF
                     RLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRP
                     AGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAV
                     CFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAEL
                     LLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            905..2440
                     /gene="gadC"
                     /locus_tag="OHO85_002740"
     CDS             905..2440
                     /gene="gadC"
                     /locus_tag="OHO85_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCQ2672092.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2571..3890
                     /locus_tag="OHO85_002741"
     CDS             2571..3890
                     /locus_tag="OHO85_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCQ2672093.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4188..4229
                     /locus_tag="OHO85_002742"
     CDS             4188..4229
                     /locus_tag="OHO85_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672094.1"
                     /translation="MVQLVDLARCVSF"
     gene            4267..5649
                     /gene="dosC"
                     /locus_tag="OHO85_002743"
     CDS             4267..5649
                     /gene="dosC"
                     /locus_tag="OHO85_002743"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCQ2672095.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5674..8073
                     /gene="dosP"
                     /locus_tag="OHO85_002744"
                     /gene_synonym="pdeO"
     CDS             5674..8073
                     /gene="dosP"
                     /locus_tag="OHO85_002744"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCQ2672096.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKETISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            8331..8912
                     /gene="ddpX"
                     /locus_tag="OHO85_002745"
     CDS             8331..8912
                     /gene="ddpX"
                     /locus_tag="OHO85_002745"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCQ2672097.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQVCPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHDRSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            8926..10476
                     /locus_tag="OHO85_002746"
     CDS             8926..10476
                     /locus_tag="OHO85_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCQ2672098.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTSVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYS
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10478..11500
                     /locus_tag="OHO85_002747"
     CDS             10478..11500
                     /locus_tag="OHO85_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2672099.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQHLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFSATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLSGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11497..12393
                     /locus_tag="OHO85_002748"
     CDS             11497..12393
                     /locus_tag="OHO85_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCQ2672100.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12390..13376
                     /locus_tag="OHO85_002749"
     CDS             12390..13376
                     /locus_tag="OHO85_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2672101.1"
                     /translation="MTQPVLDIQQLHLSFPGFHGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIDLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            13369..14295
                     /locus_tag="OHO85_002750"
     CDS             13369..14295
                     /locus_tag="OHO85_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2672102.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRV
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(14351..14782)
                     /gene="osmC"
                     /locus_tag="OHO85_002751"
     CDS             complement(14351..14782)
                     /gene="osmC"
                     /locus_tag="OHO85_002751"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCQ2672103.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            15127..15342
                     /gene="bdm"
                     /locus_tag="OHO85_002752"
     CDS             15127..15342
                     /gene="bdm"
                     /locus_tag="OHO85_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCQ2672104.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAQHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15444..15581
                     /gene="sra"
                     /locus_tag="OHO85_002753"
     CDS             15444..15581
                     /gene="sra"
                     /locus_tag="OHO85_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCQ2672105.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15738..17435
                     /gene="maeA"
                     /locus_tag="OHO85_002754"
     CDS             15738..17435
                     /gene="maeA"
                     /locus_tag="OHO85_002754"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCQ2672106.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17569..18579
                     /gene="adhP"
                     /locus_tag="OHO85_002755"
     CDS             17569..18579
                     /gene="adhP"
                     /locus_tag="OHO85_002755"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCQ2672107.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVCTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            18725..19009
                     /locus_tag="OHO85_002756"
     CDS             18725..19009
                     /locus_tag="OHO85_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCQ2672108.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(19416..20069)
                     /gene="fdnI"
                     /locus_tag="OHO85_002757"
     CDS             complement(19416..20069)
                     /gene="fdnI"
                     /locus_tag="OHO85_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCQ2672109.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(20062..20946)
                     /gene="fdnH"
                     /locus_tag="OHO85_002758"
     CDS             complement(20062..20946)
                     /gene="fdnH"
                     /locus_tag="OHO85_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCQ2672110.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20959..24006)
                     /gene="fdnG"
                     /locus_tag="OHO85_002759"
     CDS             complement(20959..24006)
                     /gene="fdnG"
                     /locus_tag="OHO85_002759"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(23419..23421),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCQ2672111.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQSEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            24238..>24972
                     /gene="yddG"
                     /locus_tag="OHO85_002760"
     CDS             24238..>24972
                     /gene="yddG"
                     /locus_tag="OHO85_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCQ2672112.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIM"
BASE COUNT         6094 a         6343 c         6345 g         6190 t
ORIGIN      
        1 agttcccaca accgctgcac gatgcgctgg ataaattcca ggccgatacc ggtatcgaca
       61 tcgacatgca catcgacgct gccagcggtg gcttcctggc accgttcgtc gccccggata
      121 tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag tgcttcaggc cataaattcg
      181 gtctggctcc gctgggctgc ggctgggtta tctggcgtga cgaagaagcg ctgccgcagg
      241 aactggtgtt caacgttgac tacctgggtg gtcaaattgg tactttcgcc atcaacttct
      301 cccgcccggc gggtcaggta attgcacagt actatgaatt cctgcgcctc ggtcgtgaag
      361 gctacaccaa agtacagaac gcctcttacc aggttgccgc ttatctggcg gatgaaatcg
      421 ccaaactggg gccgtatgag ttcatctgta ccggtcgccc ggacgaaggc attccggcgg
      481 tttgcttcaa actcaaagat ggtgaagatc cgggatacac cctgtatgac ctctctgaac
      541 gtctgcgtct gcgcggctgg caggttccgg ccttcactct cggcggtgaa gccaccgaca
      601 tcgtggtgat gcgcattatg tgtcgtcgcg gcttcgaaat ggactttgct gaactgttgc
      661 tggaagacta caaagcctcc ctgaaatatc tcagcgatca cccgaaactg cagggtattg
      721 cccagcagaa cagctttaaa catacctgat aacgtttaac ggtaacggtg tcccgaaacg
      781 aacccgtttc gggacaattt ccaaagtctg ttcactggca ttagcaacgg aaaatattgt
      841 tctgaatacg cttcagaaca aaacaggtgc ggttccgaca ggaataccgt tttaggggga
      901 taatatggct acatcagtac agacaggtaa agctaagcag ctcacattac tcggattctt
      961 tgccataacg gcatcgatgg taatggctgt ttatgaatac cctaccttcg caacatcggg
     1021 cttttcatta gtcttcttcc tgctattagg cgggatttta tggtttattc ccgtgggact
     1081 ttgtgctgcg gaaatggcca ccgtcgacgg ctgggaagaa ggtggtgtct tcgcctgggt
     1141 atcaaatact ctggggccga gatggggatt tgcagcgatc tcatttggct atctgcaaat
     1201 cgccattggt tttattccga tgctctattt cgtgttaggg gcactctcct acatcctgaa
     1261 atggccagcg ctgaatgaag accccattac caaaactatt gcagcactca tcattctttg
     1321 ggcgctggca ttaacgcagt ttggtggcac gaaatacacg gcgcgaattg ctaaagttgg
     1381 cttcttcgcc ggtatcctgt tacctgcatt tattttgatc gcattagcgg ctatttatct
     1441 gcactccggt gcccccgttg ctatcgaaat ggattcgaag accttcttcc ctgacttctc
     1501 taaagtgggc acactggttg tatttgttgc cttcattttg agttatatgg gcgtagaagc
     1561 ttccgcaact cacgtcaatg aaatgagcaa ccccgggcgc gactatccac tggctatgtt
     1621 actgctgatg gtggcggcaa tctgcttaag ctctgttggc ggtttatcta ttgcgatggt
     1681 cattccgggt aatgaaatca acctctccgc aggggtaatg caaaccttta ccgttctgat
     1741 gtcccatgtg gcaccggaaa ttgagtggac ggttcgcgtg atctccgcac tgctgttgct
     1801 gggtgttctg gcggaaatcg cctcctggat tgttggtcct tctcgcggga tgtatgtcac
     1861 agcgcagaaa aacctgctgc cagcggcatt cgctaaaatg aacaaaaatg gcgtaccggt
     1921 aacgctggtc atttcgcagc tggtgattac ttctatcgcg ttgatcatcc tcaccaatac
     1981 cggtggcggt aacaacatgt ccttcctgat cgcactggcg ctgacggtgg tgatttatct
     2041 gtgtgcttat ttcatgctgt ttattggcta cattgtgttg gttcttaaac atcctgactt
     2101 aaaacgcaca tttaatatcc ctggtggtaa aggggtgaaa ttggtcgtgg caattgtcgg
     2161 tctgctgact tcaattatgg cgtttattgt ttccttcctg ccgccggata acatccaggg
     2221 tgattctacc gatatgtatg ttgaattact ggttgttagt ttcctggtgg tacttgccct
     2281 gccctttatt ctctatgctg ttcatgatcg taaaggcaaa gcgaataccg gcgtcactct
     2341 ggagccaatc aacagtcaga acgcaccaaa aggtcacttc ttcctgcacc cgcgtgcacg
     2401 ttcaccacac tatattgtga tgaatgacaa gaaacactaa ccatgtctga atggttataa
     2461 gacaagggag cgataagtca tcgctccctt tttcgtgctt gctgtcgtat tgacttaacc
     2521 gggataagta cgagaatgag cgcacatctg tttaccggaa accagcacat atggatatct
     2581 gctcccgaaa caagaaatta acgattagaa gaccagcgat actagttgca ctggcacttt
     2641 tactgtgtag ttgtaaaagc acgcctccag agtccatggt gacaccacca gcaggttcaa
     2701 agccaccagc cacgacgcaa caatcgtcac aaccgatgcg tggcatctgg ctggccacgg
     2761 tttctcggct cgactggcca ccggtttcct cggttaacat tagtaacccc accagccggg
     2821 cccgtgtaca acaacaggcg atgatcgaca aactggatca tctgcaacgt ctcggcataa
     2881 acacggtctt tttccaggtc aagccggacg gtaccgccct gtggccatcg aaaattttgc
     2941 cgtggtccga tcttatgacc ggtaagattg gtgaaaatcc gggttacgat ccgctgcaat
     3001 ttatgctcga cgaagcccac aagcgtggga tgaaagtaca cgcctggttt aacccctatc
     3061 gcgtatcggt taatacgaag cccggtacta tcagggaact gaatagcact ctgtctcaac
     3121 aaccggcgag cgtctatgtg caacaccgcg actggatcag aacgtctggc gatcgctttg
     3181 tcctcgaccc gggcatccct gaggttcagg actggatcac atcaatagtc gcagaagtgg
     3241 tttcccgcta tcccgtagat ggcgtgcagt ttgacgacta tttctatacg gagtcaccgg
     3301 gttcacggct aaatgataac gaaacgtacc gtaaatacgg aggcgcattt gcgtcaaaag
     3361 cagactggcg gcgcaacaat actcagcagt taattgcaaa ggtatcgcac accattaaaa
     3421 gcattaagcc gggagtcgaa tttggtgtta gcccggcagg cgtgtggcgt aaccgatcac
     3481 acgatccgct cggttccgat acccgaggcg cggcagccta tgacgaatcc tacgctgaca
     3541 cccgtcgatg ggtggaacaa ggattgctgg attacattgc tccccaaatt tactggccgt
     3601 tctcacggag tgccgcgcgt tatgacgtgt tggcaaaatg gtgggcggat gtcgttaaac
     3661 cgaccaggac ccgcctgtat atcggtatcg ccttctataa agtgggtgaa ccctcaaaga
     3721 tagagccaga ctggatgatt aacggcggcg taccggaact gaaaaagcag ctcgatctta
     3781 acgatgcggt accagaaatt agcggcacca tcttgttccg tgaggactat ctgaataaac
     3841 cgcagactca acaagcggtc agctatctgc aaagtcgttg gggcagttaa actcccattc
     3901 ttactttgta acaagccggt gctttaccca ccggcttgtt accctcttga actatcccac
     3961 tactaatcat gcttactaaa gtcaaattaa ccacgcaatt aaccacactt agttaaacag
     4021 ccataaatcc cctaataaaa atgtatgaat atgaaaaaac atacgatatt cgctgtcacc
     4081 gaaaaataat tacgatgggc tatactcttc tgaagaacag tagcgtagat taaaactgtc
     4141 ctaagtcgct tatttctttt cagtatatct tcatatttca ggagaatatg gtgcagttag
     4201 tcgatctcgc acgttgcgtt tcgttttgat tccgtctgat ttcccagcct tataagggtg
     4261 tgaattatgg agatgtattt taaaagaatg aaagatgagt ggaccggact tgtcgaacag
     4321 gcagatcctc tcattcgtgc taaagccgca gaaattgccg ttgcgcatgc tcattatctg
     4381 agtatcgagt tttatcgaat tgtccgcatc gacccgcatg ccgaagaatt cttgagtaat
     4441 gaacaagttg agcggcagtt gaaaagtgcg atggaacgct ggattattaa cgtgctttct
     4501 gcccaggttg acgatgtcga aaggctaata caaatccagc ataccgtcgc ggaagtgcat
     4561 gcccgcatag gaattccggt agaaattgtc gagatggggt ttcgggtgct gaaaaaaatc
     4621 ctctatccgg taatcttctc ttcggattat tccgccgcag aaaaacttca ggtctatcat
     4681 ttctcgatta acagtattga tatcgcgatg gaagtgatga cccgcgcgtt tacctttagt
     4741 gacagtagtg cctcaaagga agatgaaaac tatcgtatct tctcgttgct ggaaaacgcc
     4801 gaagaagaaa aagaacggca aatagcctca atactttcat gggaaataga tattatctat
     4861 aaaatcctgc tggattctga tttaggcagt agtttgcctt taagccaggc tgattttggc
     4921 ctgtggttta accataaagg tcgacattat tttagtggca ttgccgaagt aggccatatc
     4981 tcccgtctga ttcaggattt cgacggtata ttcaatcaaa ccatgcgtaa caccaggaat
     5041 ctgaataaca gaagtctgcg ggtgaaattt ttattacaga taagaaatac tgtatcgcaa
     5101 attattacct tgctgcgtga attgtttgaa gaagtatcgc gccacgaagt cggtatggat
     5161 gtactgacga aattacttaa ccgccgtttc ctgccgacta tcttcaaacg cgaaattgcc
     5221 catgccaacc ggaccggtac accgctgtca gtgctgatta ttgacgttga taaattcaaa
     5281 gagatcaacg atacgtgggg ccataacact ggcgatgaaa ttctgcgtaa agtctctcag
     5341 gccttttatg acaacgtccg cagtagtgat tatgttttcc gctacggggg cgatgaattt
     5401 atcattgttt tgactgaagc ttcggaaaac gaaacgttac gtaccgcaga acgtattcgc
     5461 agtcgggtgg agaaaaccaa actgaaagcc gcaaacggcg aggatattgc cctctcactt
     5521 tccatcggtg ccgccatgtt taatggtcat cctgactatg agcgcctcat tcaaatagcc
     5581 gatgaagctc tgtatatcgc caaaagacga ggtagaaacc gtgttgaact ctggaaagcc
     5641 agtctttaga tgcgccagga tgcagaggta atcatgaagc taaccgatgc ggaaaatgcc
     5701 gccgatggca tttttttccc cgcccttgag caaaatatga tgggtgcggt gttaattaac
     5761 gaaaatgatg aagtgatgtt tttcaacccc gcggcagaga agctctgggg gtacaaacgt
     5821 gaagaagtca ttggcaataa cattgatatg ctgattccgc gggatttgcg tcctgcgcat
     5881 cctgaataca ttcgtcataa ccgtgaaggc ggtaaagcgc gcgtcgaggg gatgagtcgg
     5941 gagctgcagc tggagaaaaa agacggcagt aaaatctgga cccgttttgc gctatcgaaa
     6001 gtgagcgccg aggggaaagt ttattacctg gcgctggtac gggatgccag cgtagaaatg
     6061 gcgcaaaaag aacagacccg acaattgatt attgccgttg accatctcga ccgaccggtg
     6121 attgtcctcg atccggaacg ccatattgtg cagtgcaatc gcgcatttac cgaaatgttt
     6181 ggttactgca ttagcgaagc cagcggtatg cagcccgata cactcctgaa cattcctgaa
     6241 ttccctgccg ataaccgcat tcgtttacaa cagttgctat ggaaaaccgc ccgcgatcag
     6301 gacgaatttc tgctgttgac gcgcaccggt gaaaaaatct ggattaaagc ctctatcagc
     6361 ccggtttatg acgtgctcgc gcatctgcag aacctggtaa tgactttctc ggatatcacc
     6421 gaagaacggc agattcgcca gcttgaaggc aatattctcg ccgccatgtg cagcagcccg
     6481 ccatttcatg aaatggggga aatcatttgt cgtaacatcg aatctgtact caacgaatcg
     6541 catgtttcgc tgttcgcact gcgcaacggg atgccgatac actgggcgtc atcttcgcac
     6601 ggtgcagaag ttcaaaatgc gcaaagctgg tcagcgacca ttcgtcagcg tgatggcgcg
     6661 cctgcgggga tcctgcaaat taaaacctcg tcaggagcag aaaccagcgc ctttatcgaa
     6721 cgcgtggcag atatcagcca gcatatggcc gcgctggcgc tggaacagga aaaaagccgt
     6781 cagcatattg aacaactcat ccaatttgat ccgatgaccg gtctgccaaa tcgcaataac
     6841 ctgcacaatt acctcgatga cctggtcgac aaagccgtct ctccggtggt gtatctcatc
     6901 ggtgttgacc atattcagga tgtgattgat agccttggct atgcgtgggc cgatcaggca
     6961 ttgctggaag tggtcaatcg ctttcgtgaa aaactcaaac cggatcagta tctctgtcgt
     7021 atcgaaggta cgcagtttgt cctcgtgagc ctggaaaacg acgtcagtaa cattacccag
     7081 atcgccgatg agctacggaa tgtggtcagc aagccgataa tgattgacga taaacccttc
     7141 ccgcttacct tgagtattgg catcagctac gacgtgggga aaaaccgcga ttacctgctc
     7201 tccactgccc ataacgcgat ggattatatt cgcaagaatg gcggtaacgg ctggcagttc
     7261 ttcagcccgg cgatgaacga aatggtaaaa gagcgtttgg ttttaggcgc agcgctgaaa
     7321 gaaacgatta gcaataacca actgaaactg gtttaccagc cgcaaatctt cgcagaaacg
     7381 ggtgaactgt acggcatcga agcccttgct cgctggcacg atccccagca tggtcatgta
     7441 cccccttcac ggtttattcc tctcgcagaa gagattggtg aaatcgaaaa tattgggcgc
     7501 tgggtcatcg cggaagcttg ccgtcagtta gcagaatggc gtagccagaa tattcatatc
     7561 ccggcgttat ccgtgaactt gtcggcgctg cactttcgca gtaatcaact gcctaatcag
     7621 gtgtctgatg caatgcaagc ctggggtatt gacggccacc agctgacggt ggaaatcacg
     7681 gaaagcatga tgatggaaca cgataccgaa atctttaagc gcattcagat cctgcgtgat
     7741 atgggcgtgg gtttatcggt agatgatttt ggcacgggct tttccggatt atcccgctta
     7801 gtcagtcttc cggtaacgga aatcaaaatt gacaaaagtt ttgtcgatcg ttgtctgacg
     7861 gaaaaacgca tccttgcctt acttgaagcc attaccagca ttgggcaaag cctcaattta
     7921 accgtcgtgg cggaaggcgt cgaaaccaaa gagcaatttg agatgctacg caagatccac
     7981 tgtcgcgtta ttcagggata tttcttttcc cgccccctgc ccgccgaaga aattccaggc
     8041 tggatgagca gcgtgttacc gctgaaaatc tgacaaattc ctctcgcccg cactcgcggg
     8101 tattcattta acgtgacact gtcacttaaa tactgtgatt tcagctacga ttccgggaga
     8161 ttccttcctt aacttcccct cttgcaccaa aactgcacta cgctgcacaa atgttgatca
     8221 aaagttaaaa aatataactt taagccagca ttctggcgct tctcccggca tggcatgaga
     8281 tctgcataag cggaaagcgc agcaattttt gtcttataca ggaaccgttt atgtcggata
     8341 ccaccgaact ggttgattta gccgtaatct tccctgatct ggagatcgaa ttgaaatacg
     8401 cctgcgctga taacatcaca ggtaaagcta tttatcagca agcgcgttgt ctgttacaca
     8461 aggatgcgat taccgcgctg gcgaaaagta tcagtatcgc ccagctgtca ggcttacaac
     8521 tggtgattta cgatgcgtat cgcccacaac aagcacaggc gatgttgtgg caagtctgcc
     8581 cagacccgca atatgttgtt gatgtgacgg tcggttctaa tcacagccgt ggcacggcga
     8641 tcgacctgac gcttcgtgat gagcacggga acatcctcga tatgggcgca gggttcgatg
     8701 aaatgcacga tcgctcccat gcttaccatc cttccgtccc gcccgccgct cagcgcaatc
     8761 gcctgttgct gaatgcgatt atgactggtg gcggttttgt cggtatctcc agcgaatggt
     8821 ggcacttcga attaccccag gcagcgagtt acccactgct cgctgatcaa ttcacctgtt
     8881 ttatatcccc tggcacacag cacgtcagtt aatcaggaaa ctgtcatgaa aagatcgata
     8941 tcgtttcgtc ctacattgct cgcgctcgtc cttgccacaa ctttcccggt tgcgcacgcc
     9001 gccgtaccga aagatatgct ggtgatcggt aaggctgccg atccacaaac cctcgacccg
     9061 gcggtgacaa ttgataataa cgactggacg gtgacctacc cgtcttatca gcggctggtt
     9121 cagtacaaaa cggacggtga taaaggctca accgacgttg aaggcgatct ggcaagtagc
     9181 tggaaagcgt ctgacgatca aaaagagtgg acgttcaccc tgaaggataa tgctaaattt
     9241 gccgatggca catctgtcac tgccgaagca gtaaaactct cttttgagcg gttactaaaa
     9301 atcggccagg ggccagcaga agcatttccc aaagatttaa agattgatgc tcccgacgaa
     9361 catacggtga agtttaccct tagccaacca ttcgcaccgt tcctctacac gctggcgaat
     9421 gacggtgcat ccattatcaa tccggcggtg ttaaaggaac atgcagcgga tgatgctcgc
     9481 ggcttcctcg cgcaaaatac cgccggttcc ggaccattta tgctgaaaag ctggcaaaaa
     9541 ggtcagcaat tagttctggt gccaaatccg cattactccg gcaataaacc gaactttaag
     9601 cgagtatcgg taaaaattat cggtgaaagt gcctcccgtc gcctgcagct ctcccgtggc
     9661 gacattgaca ttgccgatgc gctgccggtg gatcaactca acgccctgaa gcaggaaaat
     9721 aaagtcaatg tggcagagta tccgtcactg cgcgtcacct atctgtatct gaataacagc
     9781 aaagcgccac ttaatcaggc ggatctgcgt cgggccattt cctggtctac cgattatcag
     9841 ggcatggtta acggcattct gagtggtaac ggaaaacaaa tgcgcggccc gattccggaa
     9901 ggcatgtggg gctacgatgc gacggcaatg caatacaacc atgacgaaac gaaagccaaa
     9961 gccgaatggg ataaagtgac gagcaaaccc accagcctga cgtttctcta ctccgataac
    10021 gatccgaact gggaaccgat cgctctggcg acacaatcca gtctcaacaa gctgggcatc
    10081 aatgtgaagc tggaaaagct ggcgaacgcc accatgcgcg acagagtggg taaaggtgat
    10141 tacgacattg cgattggcaa ctggagtccg gattttgccg acccgtatat gtttatgaat
    10201 tactggtttg agtcagacaa aaaaggtctg ccgggtaacc gctcgttcta tgaaaacagt
    10261 gaggtcgata agttactgcg caatgcgctt gcgaccaccg accagacgca gcgtacccgg
    10321 gactaccagc aggcacagaa aatcgtcatt gatgacgctg cttatgtgta cctgttccag
    10381 aaaaactacc aactggcgat gaacaaagag gtgaaaggct ttgtgttcaa tcccatgctg
    10441 gaacaggtct tcaatatcaa taccatgagt aaataacgtg accttctgga gtattttacg
    10501 ccaacgctgc tgggggctgg tgctcgtggt ggcgggcgtc tgcgtgatta cgtttattat
    10561 ctcgcatctg atccctggtg atccggcgcg gttactggcg ggtgaccgcg ccagcgatgc
    10621 tatcgtggaa aatattcgcc agcatctggg actggaccag ccactgtacg tacagtttta
    10681 ccgctacgtc agcgacctgt ttcatggtga cctcggaaca tccattcgta ccgggcgtcc
    10741 ggtgctggaa gagttgcgta tatttttctc ggcgacgctg gaactggctt tttgtgccct
    10801 gctgctggca ctcctgattg gcattccgct gggcatactc tctgccgtct ggcgaaatcg
    10861 ctggctggat catctggtgc gaataatggc cattaccgga atctccacac ctgcgttctg
    10921 gcttgggctg ggcgtcattg tgctgtttta tggtcatctg caaattcttc ccggcggcgg
    10981 aaggcttgat gactggctgg atccgccaac gcacgttacc ggcttttatc tgctcgatgc
    11041 gctgcttgaa ggcaacggtg aagtcttctt caatgcgttg caacatctca tcttaccggc
    11101 attaacgctg gcgtttgttc acctggggat tgtcgcccgc cagatccgtt cagcgatgct
    11161 ggaacaattg agtgaagact acattcgtac cgccagggcc agcggcttgt ccggctggta
    11221 tatcgtttta tgttatgcgc tacccaatgc gttgatccca tcgattaccg tattgggtct
    11281 ggcgctgggc gatttgttgt atggcgcagt gctcaccgaa accgtttttg cctggcccgg
    11341 aatgggtgca tgggtagtga catcaataca ggcgctcgac ttcccggctg tgatgggctt
    11401 tgccgtcgtg gtttcatttg cttatgtgct ggtcaacctg gtggtggatt tgctctattt
    11461 gtggattgat ccgcgtatcg gacgtggagg tggtgaatga tgctaagcga ggaaacgtcc
    11521 gccgtacgtc cacaaaaaca aacgcgattt aatggtgcaa aactggtatg gatgctgaaa
    11581 ggcagtccgc tcaccgtgac cggcgcagtc atcattgtat taatgctact gatgatgatt
    11641 ttttcaccgt ggctggcgac gcatgatccc aacgccattg atttaaccgc ccgccttttg
    11701 ccgccttctg cggcgcactg gtttggcacc gatgaagtgg gacgcgatct gttcagccgc
    11761 gtactggtcg gcagtcagca atcaattctc gccggattag tggtggtcgc cattgcgggt
    11821 atgattggtt cgctactcgg atgtctatcc ggtgtgcttg gcggacgcgc agacgccatc
    11881 atcatgcgca tcatggacat tatgctgtcg attccttcgt tggtactgac aatggcactg
    11941 gctgccgctc tcgggccgag tttgtttaac gccatgctgg ctattgctat tgtgcgaatt
    12001 cccttttatg tgcgcctggc gcgggggcaa gcattagttg tacgccagta tacctatgtt
    12061 caggcggcga aaacctttgg cgcgtctcgt tggcatctga tcaactggca tattttacgt
    12121 aactccctac cgccgctgat cgtgcaggca tcgctggata tcggtagcgc gattttaatg
    12181 gccgccacgt tgggatttat tggcctgggt gctcaacaac cgagtgctga atggggcgcg
    12241 atggtagcaa atggtcgcaa ctatgtactc gatcagtggt ggtattgcgc atttccgggg
    12301 gcagcgattt tgcttaccgc cgtcgggttt aatctctttg gtgatggtat tcgcgatctg
    12361 cttgacccga aagcaggagg aaagcagtca tgacccaacc cgttctggac attcaacaac
    12421 tgcatttgag tttccccggt tttcacggtg atgttcacgc gctcaacaat gtgtccttgc
    12481 agattaaccg cggtgaaatt gtcggtctgg tgggagaatc cggctcaggt aaatcagtca
    12541 ccgcaatgct gattatgcgt ctgctaccga cgggcagtta ttgcgtacat cgggggcaga
    12601 tttccctgct gggagaagat gttcttaacg cgcgggaaaa acagcttcgt cagtggcgcg
    12661 gcgcacgagt ggcgatgatc tttcaggaac cgatgaccgc cctcaatccg acacgtcgaa
    12721 taggtcttca gatgatggac gtgatccgcc atcatcaacc aataagtcgt cgggaagcca
    12781 gagctaaagc gattgacctg ctggaagaga tgcaaatccc ggatgccgtg gaagttatgt
    12841 cgcgctatcc gtttgagctt tcaggtggta tgcgccagcg ggtaatgatt gcgctggcat
    12901 tctcctgcga gccgcaattg attattgccg acgaaccgac tacggcgctg gacgtcacgg
    12961 tgcagttgca ggtactgcgt ttgcttaaac ataaagcccg cgccagtgga actgcggttc
    13021 tgttcatcag ccatgatatg gccgtggtgt cgcaactgtg cgatagcgtt tacgtgatgt
    13081 atgccggaag cgtaattgaa agcggcgtga cggcagacgt tatccatcat ccccggcatc
    13141 cgtataccat tggtttgctg caatgcgcac cagaacatgg agtaccacgc cagctattac
    13201 ccgccattcc agggacggta ccaaacctca cccatttgcc tgacggctgc gcttttcgcg
    13261 atcgttgcta tgcggcaggt gcacagtgtg aaaacgtccc ggcgctgaca gcgtgtggtg
    13321 acaacaacca gcgctgcgcc tgttggtatc cgcagcagga ggtcataagt gtctgacacg
    13381 ttattaacgt tacgcgacgt ccatatcaat ttcccggccc gtaaaaactg gcttggtaaa
    13441 actacggaac atgttcatgc cattaatggt attgatttac agatccgccg tggtgaaacc
    13501 ttagggatcg tcggcgagtc aggctgcggc aaaagcaccc tcgcacagct tttaatgggt
    13561 atgctgcaac cgagccacgg gcagtacatc cgttctggct cacaacgcat tatgcagatg
    13621 gtgtttcagg acccgctctc ttcgcttaat ccgcgcttac cggtgtggcg cgtcatcaca
    13681 gaaccgctct ggatagctaa gcgtagtagt gaacaacagc ggcgagcgtt ggcagaggag
    13741 ttggctgtgc aggtgggtat tcgtccggag tatctcgacc gcctgcctca tgcgttctcc
    13801 ggcgggcagc ggcaacgtat cgccattgcc agagcactct cttcgcagcc tgacgtgatt
    13861 gtgcttgatg agccaacctc tgcgctggat atctccgttc aggcgcagat cctcaattta
    13921 ctggtaacgc tacaggaaaa tcacgggctg acctatgtgc tgatttcaca caatgtctcg
    13981 gtgatacgtc atatgagcga tcgggtggcg gtgatgtatc tcgggcagat tgtagaactg
    14041 ggcgacgcgc agcaggtgct gacggcacct gcacatccat acacccgatt attgctggat
    14101 tccctccccg ccattgataa accgctggag gaagaatggg cattacgtaa aacggatctg
    14161 ccgggaaacc gcacgttgcc gcaaggctgt tttttctacg aacgttgccc gctggcaacc
    14221 cacggatgtg aagtccggca atcattagca ataagggagg acggacgtga gctccggtgc
    14281 tggcgggcgc tgtagagatg ctaacaacgc atcaggcatt cacgcgcctg acgcgtcatc
    14341 cggcaatgct ttacgatttc aactggtaat ccagcgtaat ttccgctttc agtacctgag
    14401 agaccgggca tcctgctttt gctttctgga tgatgccgtc aaaggtagag gcatcaatac
    14461 ccggcaccgc aacttcactc ttcagtgcga ttttcgtaat cgcaaaaccg gcatccactt
    14521 tatccagcga cacatcggcg gtggtatcaa tcgatgttgg cgtgaatccc gcttccccca
    14581 gcattaatga aagcgccatt gagaaacatg cggcatgcgc tgcgccaatc agttcttcag
    14641 ggttggttcc tttttcgcct tcaaaacgcg tgttaaatcc atacggctgt tggttcagca
    14701 cgccactctc ggtggatact gttcccttcc cgcgtttgat atcgccttcc cagtgtgcct
    14761 gacctttctt atggattgtc attgttgctc tcctgtgggc ttaaaaagga aattatagac
    14821 tacgtgagaa acagcacgag gataagcagg atattccgaa taaaatccgg cgaaaatatt
    14881 acttctgcac ttaaattaaa ttcccacaat agattaccgg gaatatataa cccaaaagat
    14941 attgtatcaa tattaatcgg atcataccaa tcacaatccc tatgaatatc atggtgttaa
    15001 aatataaaaa tcatcaacca ggactaatct taacaacgaa gcggcaaata tttgccatgc
    15061 tgaatgtgct ttataaatct gcgatccgta gcagacacca taaatacaca gacacggaga
    15121 atcactatgt ttacttatta tcaggcagaa aattcaacag cagaacccgc tctggttaat
    15181 gccattgagc aaggccttcg ggcacagcac ggtgttgtca ctgaagatga cattctcatg
    15241 gagctgacca aatgggttga agcttctgat aacgacatcc tcagtgatat ctaccagcaa
    15301 acgatcaatt atgtggtcag tggccagcac cctacgcttt aaggtgctat gcttgatcgg
    15361 caacctaatt taggggttta gcacgtgttt cttcgctacg gcgatgttgt ccttaaaact
    15421 agcaacagga ttgaggagtt aaaatgaaat cgaaccgtca ggcacgtcat attcttggac
    15481 tggaccataa aatttctaac cagcgcaaaa tagttaccga aggtgacaaa tccagcgtgg
    15541 taaataaccc aaccggcaga aaacgccccg ctgaaaagta attcataacc atcagtcctc
    15601 aatgacgatt aaacaccatt gcctgcgcaa tggtgttttt gtttttatct gctttatact
    15661 tggggccgac gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc
    15721 aaaaaagagt gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt
    15781 acgctggccc tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg
    15841 aagaacgccg taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag
    15901 aacaagcgga acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca
    15961 tctacctgcg taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc
    16021 atcttgatga gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt
    16081 tttctgagat ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cggcacaata
    16141 tggacgatat tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg
    16201 gtgaacgtat tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta
    16261 aactgtcgct ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc
    16321 tggatgtcgg aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta
    16381 atccgcgtat cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga
    16441 aacaacgctg gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc
    16501 cgttacttaa ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg
    16561 cggcagtaac agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg
    16621 agaaaaaaat cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca
    16681 tcgcccagac ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg
    16741 tcgatcgctt tggcctgctg acggacaaga tgccgaacct gctacctttc cagaccaaac
    16801 tggtgcagaa gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc
    16861 tggatgtggt gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc
    16921 tgtttacgga agagatcatt cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc
    16981 cgctgtctaa cccgacgtca cgcgtggaag ccacaccgca ggacattatc gcctggaccg
    17041 aaggtaacgc gctggtcgcc actggcagcc cgtttaatcc agtggtatgg aaagataaaa
    17101 tctaccctat cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggggtta
    17161 ttgcttccgg cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg
    17221 ctcagtattc gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata
    17281 ttcagaaagt ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg
    17341 tggcggtgaa aacatctgcc gaagctctgc aacaggccat tgatgataat ttctggcaag
    17401 ccgaataccg cgactaccgc cgtacctcca tctaagcctg cgcccggtag tgaaggctac
    17461 cgggctattt ctctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc
    17521 tcatttgcct cacctgctat gcagaacatc atccgaaaag gaggaactat gaaggctgca
    17581 gttgttacga aggatcatca cgttgacgtt acggataaaa cactgcgctc actgaaacat
    17641 ggcgaagccc tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag
    17701 aatggcgatt ttggtgacaa aactggcgta attctgggcc atgaaggcat cggtgtggtg
    17761 gcagaagttg gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg
    17821 ttctacgaag gatgcggtca ttgtgaatac tgtaatagtg gtaacgaaac gctctgccgt
    17881 tcagttaaaa atgccggata cagcgttgat ggcgggatgg cggaagagtg catcgtggtc
    17941 gccgattacg cggtaaaagt gccagatggt ctggactcgg cggcggccag cagcattacc
    18001 tgtgcggggg tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc agggcagtgg
    18061 attgctatct acggtcttgg cggtctgggt aacctcgctc tgcaatacgc gaagaatgtc
    18121 tttaacgcga aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa
    18181 atgggtgcag atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag
    18241 aaaaccggtg gcgctcacgc tgcggtggta acagcagttg ctaaagctgc gtttaactcg
    18301 gcagttgatg ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct
    18361 atgagcctgg atatcccacg gctggtgctg gatggcattg aagtggtcgg ttcgctggtc
    18421 tgcacgcgcc aggatttaac tgaagccttc cagtttgccg ccgaaggtaa agtggtgccg
    18481 aaagtcgccc tgcgtccgtt agcggacatc aacaccatct ttactgagat ggaagaaggc
    18541 aaaatccgtg gccgcatggt gattgatttc cgtcactaag aggcctttgc tgcgactgcc
    18601 atgttcgggt cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg
    18661 cgtaggaagg aacaataatg atagtgttcc cagtgaagga acgctactga gagtaaagac
    18721 actaatgaaa atggccaatc atccccgccc gggggacatt attcaggaat cactggacga
    18781 acttaatgtc agcctgcgcg agtttgccag agcaatggaa attgctccct caacggcaag
    18841 ccgattgctg acaggaaaag cagctttgac gccagaaatg gcgattaaac tttccgtggt
    18901 gatcggcagt tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc
    18961 agaaaaaaca gtggatgtgt cgcgattacg ccgtctggtt acgcaataaa aaaatggcac
    19021 tttataaagc gccatttaga actgatgaca aacgcaacat tgcctgatgc gctacgctta
    19081 tcaggcctac acgttcactg caatctattg gatttgaagg ggttgtaggc cggataaggc
    19141 gttcacgccg catccggcat gaacaaagcc tacgttgtct acaatatgaa aatgacgcct
    19201 tttacagcgc catttgaaac tgatgacaaa cgcaacattg cctgatgcgc tacgcttatc
    19261 aggcctacac gttcactgca atctattgga tttgaagggt ttgtaggccg gataaggcgt
    19321 tcacgccgca tccgggatga acaaagccta cgttgtctac aatatgaaaa tggcgccttt
    19381 tacagcgcca ttttcagctt aaagttcaaa agagattata tcccttcttc actctctttt
    19441 ttcgcttctg ccttctcgat ttcacgatac cagcgtggat ggtgtttctt cgcccagcga
    19501 cggctcacct tcccttcgat catcccttta atcgaccctt tcacccaaaa tgccatatac
    19561 atatggatca ggatggcgtg gatcaggatg atacccgcag ccgcgtggat cagcaggctg
    19621 tagcgaacaa cctgcatcgg gaagtactgc gcaaagtacg gacgccagat aatcaccccg
    19681 gtcaccagca gcacgaaaat catgctcatg atcgaccaga acatcatctt ttgcccggcg
    19741 ttgtacttac cgacatccgc cactttatgc tcattgcctt tcaatacttc gacaatgttc
    19801 aacagccacg gaatatcttt cttatccggg atgttgtgat gcacaaaacg cacaaacata
    19861 aacatcagtg cgacgaaaat cgcaatgccg aagaacgggt gcaaaatgcg tcccatctgc
    19921 ggcgtaccga aggtttgcgt cagccattgc agcgtcggga agaagaacga aatcccggac
    19981 agcgccacca ggaagaagca aatcaccacg gtccagtgac aggcgcgatc aataaatttg
    20041 gtgcgcacaa tcattttcga cttactcatg atgatcctcc tcgtcatcgt ccacttcctt
    20101 attcgggcca ataccgatgt agtggaaaat caacccggca aaagtggcaa taaagccagc
    20161 cgctgccagc ggtttcaacg cgcctttcca caggcttacc gaggtgtcga tcttcggatc
    20221 tttcggcaga ccgtgataca gctccggctg atcggcgtga tgcagcacgt acataacgtg
    20281 cgtaccaccg accccttccg ggttgtagac gccagcatgt tcgtaaccac gcgctttcag
    20341 tttcgccacg cgctgttccg ccagctccag catctccttc ttggtgccga agtggatagc
    20401 cccggtcgga caggttttca cacaagccgg ttcctggccg acgctgacgc gatcgacgca
    20461 gagcgtgcat ttatataccc ggttatcctc tttgttgagg cgcggaatat taaacggaca
    20521 cccggcaatg cagtaaccac agccgatgca gttttccgac tggaaatcga caatcccgtt
    20581 agcgtactga atgattgcac cagcagacgg gcacgccttc aggcagccgg gatcttcaca
    20641 gtgcatacag ccgtctttac ggatcagcca ctccagcttg ccgttctgtt cggtttcgct
    20701 aaagcgcatc accgtccagg acttggcgct cagatcggcg gggttatcgt aaaccccgac
    20761 gcagtgcccc acttcatcac ggatgtcgtt ccactccgaa cacgccacct gacaggcttt
    20821 acagccgata caggtggaaa cgtcgataag ttttgcgact tctgctttgt aatcacgcac
    20881 ctgagaaggc ggcgtgatgg agttagttgc ggaccttttg ataatgtcct gcgtttccat
    20941 agccatctgt tcgccccctt acgccttctc gatgttgact aagaacgctt tatattccgg
    21001 cgtttgcgag tttgcatcac cgacattcgg cgtcagagtg ttagcgatat aacctttacg
    21061 cgcgacaccc tcaaagcccc agtggattgg aatacccacc gtttcaacct gctgaccatt
    21121 tacattcagc ggtttcagac gacgcgttac cacagccacc gcgcggataa agccacgctt
    21181 gctggagaca gtgacacgat cgccattatt aatgcctttc gccgccgcca gcgtttcgct
    21241 gatttccaca aactgttccg gctgagcaat tgcgttgagc aatgcgtgct tggtccaggt
    21301 gtggaagtgc tcggtcagac gataggtcgt acccacatac gggaactgct cttttttacc
    21361 catccgcagc gcgtcttgtt catacagacg aacaaccggg ttagacacca cgttcggatg
    21421 cagcgggtta gtgcccagcg gcgtttcaat cggctcgtag tgttccggga acggaccttc
    21481 cgccattttg ttgatggcaa acaggcgtcc catcccttcc ggctgcatga taaacggccc
    21541 ggttggcgta cccggtgcgg cattgccgaa gtcaggaata tcgttacccg tccacttgct
    21601 gccgttccac tggatcagca tccgtttcgg atcccacggt ttaccgttga tatccgccga
    21661 agcacggttg tacagcacgc gacggttgag cggccacgcc caggcccatc ccagcgtatt
    21721 ccccagaccg gacgggtcgg agttatcgcg gttagccatc tggttgccct gctctgtcca
    21781 gctaccggtg tagatccagc aagaagatgc ggttgtaccg tcatcacgca gatgcgcaaa
    21841 gctactcagc aactgacctt tcttcgcaat aagcacgcca ttagcgtcat agagatcttc
    21901 cagcgcgtag ccgttgttct ctttagccac ttcgtcagat tgcggttcgt gcggctgctt
    21961 gtagttccag ctcatcttca tcagcggttc tacgccttta ccaccttcgg actggtacag
    22021 ctcgcgcaga tgatggtaga tacccgccag aatttcaccg tcgttacgcg cttcgcccgg
    22081 cgcgtcctga cctttccagt gccactgcaa ccagcgaccg gagttagcga tagaaccatc
    22141 ttcttcagca aagcaggtcg aaggcagacg gaatacttca gtctgaatag acgccggatc
    22201 gacatcgttc gactcaccgt ggttctgcca gaaggtagag gtttcagtca ccagcggatc
    22261 gataaccacc atgtacttca gcttgctcag gcagctcacc actttgtttt tgtccgggaa
    22321 ggacgcaacc gggttaaagc cctggcagaa ataaccggtg actttgcctt catccatcat
    22381 gttgaaatac ttgatgacgt cgtaggtctg gtcccacttc ggcagccagt catagcccca
    22441 gttgttctct ttctgcgcgg catcgccata gaaagatttc atcaggctaa cgaagaactt
    22501 cggatagttg ctccagtagt tcacctgatc agccagcgtc gctttcggcg tgttcgcttc
    22561 cagatacgac tgcaaatcaa cctgtttttc tgacggcagc gtcagataac ctggcaggct
    22621 ggtagagagc aggcctaagt cagtcaagcc ctgaatgttg gagtgaccac gcaatgcgtt
    22681 cacgccgcca ccggccatac ccatgttacc gagcagcaac tggatcatcg ccatagtacg
    22741 gatgttctgc gcacccacag tgtgctgcgt ccagcccagc gcgtacagga aggtggttgt
    22801 gcgatccggt gcgctggtgg aggccagcac ttcacacact ttcaggaagt cggcttttgg
    22861 cgtaccgcag atgttttcaa cgacgtccgg cgtgtagcgg gaaacgtgct ctttcagcag
    22921 gttccacaca cagcgcggat gagtcagtgt ttcatcgcgt ttcgcatagc cgttttcatc
    22981 gaactgatag ttccaggacg atttatcgta ttgacgtttt tcagcgtcgt agccgctgaa
    23041 cagaccgtct tcgaaagcaa aatcatcacg caccagcagg ctggcgttgg tgtaatgctt
    23101 aacgtattcg gcgttgattt tgttgttttc gatcaggtag cgcaaaacgc cagacaggaa
    23161 cgtaatgtcc gtaccggaac gaataggcgc gtaaatatcc gccacagaag cggtacgcgt
    23221 aaaacgggga tcgacaacga tcaaggttgc atcgttgttg tttttcgctt ccatcgccca
    23281 gcggaaaccg acgggatgcg cttcagcagc gttaccgccc atcaccatca cgacgttagc
    23341 gtttttgata tccacccagt ggttggtcat cgcaccgcga ccaaatgttg gagcaagact
    23401 tgctaccgtt ggtccgtgtc agacgcgcgc ctggttgtct accgccagca tcccgaggga
    23461 gcgggcaaat ttctgggtca gcatcccggt ttcgttgctg gcaccggagg cacacagcat
    23521 accggtagaa agccaacggt ttaccgttac gccctgctcg ttcttttcaa taaagttagc
    23581 gtcacggtca gctttcatca gcttcgcaat acgggagaat gcttcttccc agctaatgcg
    23641 ctgccatttg tcagaacctg gcgcacgata ttccgggtag cgcagacggt tttcactgtt
    23701 gacgtaatcc agcaaaccgg cccctttcgg gcacagcgca ccacggctta ccggatgatc
    23761 cgggtcacct tcaatgtgat aaatcgcttc tctggcgttt tttgcgccat cacccaggct
    23821 atacatcaat agcccgcaac ctacggaaca gtatgtgcag gtgttacgga tctctttagc
    23881 gcgtaataat ttgtagtttc gcgcctgagc cagtgcttgc ttcggggcaa agcccaatgc
    23941 cgctactgtt gttccagcca taccgcccgc gcagatttta aaaaattgtc tgcgactgac
    24001 gtccattgct ttcctctttt ttcaggggta ttactgcgcg aggaaactaa cagtaaaatt
    24061 ctgaatcttt ttgcgcgaaa tcaaaaagaa cacgtgtttg ccacaaaata atccgcctgc
    24121 gcaaattgac ctacctcaat agcggtagaa aaacgcacca ctgcctgaca ggccagttaa
    24181 aaaaatgcta taaaattcag cttaattttt aacggcaaga gagacaaaac agcgagcatg
    24241 acacgacaaa aagcaacgct catagggcta atagcgatcg tcctgtggag cacgatggta
    24301 ggattgattc gcggtgtcag tgaggggctc ggcccggtcg gcggcgcagc tgctatctat
    24361 tcattaagcg ggctgctgtt aatcttcacg gttggatttc cgcgtattcg gcaaatcccg
    24421 aaaggctatt tactcgccgg gagtctgtta ttcgtcagct atgaaatctg tctggcgctt
    24481 tccttagggt atgcggcgac ccgtcatcag gcgattgaag tgggtatggt gaactatctg
    24541 tggcccagcc tgacaattct ctttgccatt ctgtttaatg gtcagaaaac caactggttg
    24601 attgtacctg gattattatt agccctcgtc ggcgtctgct gggtgttagg cggtgacaat
    24661 gggttacatt atgatgaaat catcaataat atcaccacca gcccattgag ttatttcctg
    24721 gcgttcattg gtgcgtttat ctgggcagcc tattgcacag taacgaataa atacgcacgc
    24781 ggatttaacg gaattaccgt ttttgtcctg ctaacgggag catgtctgtg gatttactat
    24841 tttctcacgc cacaaccaga aatggtattt agcacgcccg tcatgattaa actcatctct
    24901 gcggcattta ccttaggatt tgcttatgct gcatggaatg tcggtatatt gcatggcaat
    24961 gtcaccataa tg
//
LOCUS       DAJOWB010000063        24715 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJOWB010000063 DAJOWB010000000
VERSION     DAJOWB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.063"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            39..671
                     /gene="comR"
                     /locus_tag="OHO85_002761"
     CDS             39..671
                     /gene="comR"
                     /locus_tag="OHO85_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCQ2672113.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(733..1272)
                     /locus_tag="OHO85_002762"
     CDS             complement(733..1272)
                     /locus_tag="OHO85_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCQ2672114.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1499..2803)
                     /gene="ndh"
                     /locus_tag="OHO85_002763"
     CDS             complement(1499..2803)
                     /gene="ndh"
                     /locus_tag="OHO85_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCQ2672115.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3211..3753)
                     /locus_tag="OHO85_002764"
     CDS             complement(3211..3753)
                     /locus_tag="OHO85_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCQ2672116.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3776..4801)
                     /gene="nagZ"
                     /locus_tag="OHO85_002765"
     CDS             complement(3776..4801)
                     /gene="nagZ"
                     /locus_tag="OHO85_002765"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCQ2672117.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDSRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4812..5636)
                     /gene="thiK"
                     /locus_tag="OHO85_002766"
     CDS             complement(4812..5636)
                     /gene="thiK"
                     /locus_tag="OHO85_002766"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCQ2672118.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5617..6258)
                     /gene="lpoB"
                     /locus_tag="OHO85_002767"
     CDS             complement(5617..6258)
                     /gene="lpoB"
                     /locus_tag="OHO85_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCQ2672119.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6272..6649)
                     /locus_tag="OHO85_002768"
     CDS             complement(6272..6649)
                     /locus_tag="OHO85_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCQ2672120.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPITVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6652..7011)
                     /gene="hinT"
                     /locus_tag="OHO85_002769"
     CDS             complement(6652..7011)
                     /gene="hinT"
                     /locus_tag="OHO85_002769"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCQ2672121.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7345..9399
                     /gene="fhuE"
                     /locus_tag="OHO85_002770"
                     /pseudo
     CDS             7345..9399
                     /gene="fhuE"
                     /locus_tag="OHO85_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(9593..11026)
                     /gene="ptsG"
                     /locus_tag="OHO85_002771"
     CDS             complement(9593..11026)
                     /gene="ptsG"
                     /locus_tag="OHO85_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCQ2672122.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11321..12118)
                     /locus_tag="OHO85_002772"
     CDS             complement(11321..12118)
                     /locus_tag="OHO85_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCQ2672123.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVNFCLAVATTL
                     PGYLHMRYLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDSSRLQSIR"
     gene            complement(12129..13133)
                     /gene="holB"
                     /locus_tag="OHO85_002773"
     CDS             complement(12129..13133)
                     /gene="holB"
                     /locus_tag="OHO85_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCQ2672124.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13130..13771)
                     /locus_tag="OHO85_002774"
     CDS             complement(13130..13771)
                     /locus_tag="OHO85_002774"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCQ2672125.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13761..14783)
                     /gene="yceG"
                     /locus_tag="OHO85_002775"
     CDS             complement(13761..14783)
                     /gene="yceG"
                     /locus_tag="OHO85_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCQ2672126.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14786..15595)
                     /gene="pabC"
                     /locus_tag="OHO85_002776"
     CDS             complement(14786..15595)
                     /gene="pabC"
                     /locus_tag="OHO85_002776"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCQ2672127.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLHDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15715..16956)
                     /gene="fabF"
                     /locus_tag="OHO85_002777"
     CDS             complement(15715..16956)
                     /gene="fabF"
                     /locus_tag="OHO85_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCQ2672128.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17044..17280)
                     /gene="acpP"
                     /locus_tag="OHO85_002778"
     CDS             complement(17044..17280)
                     /gene="acpP"
                     /locus_tag="OHO85_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCQ2672129.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17491..18225)
                     /gene="fabG"
                     /locus_tag="OHO85_002779"
     CDS             complement(17491..18225)
                     /gene="fabG"
                     /locus_tag="OHO85_002779"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCQ2672130.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18238..19167)
                     /gene="fabD"
                     /locus_tag="OHO85_002780"
     CDS             complement(18238..19167)
                     /gene="fabD"
                     /locus_tag="OHO85_002780"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCQ2672131.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19183..20136)
                     /gene="fabH"
                     /locus_tag="OHO85_002781"
     CDS             complement(19183..20136)
                     /gene="fabH"
                     /locus_tag="OHO85_002781"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCQ2672132.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AVPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20204..21274)
                     /gene="plsX"
                     /locus_tag="OHO85_002782"
     CDS             complement(20204..21274)
                     /gene="plsX"
                     /locus_tag="OHO85_002782"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCQ2672133.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21355..21528)
                     /gene="rpmF"
                     /locus_tag="OHO85_002783"
     CDS             complement(21355..21528)
                     /gene="rpmF"
                     /locus_tag="OHO85_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCQ2672134.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21580..22101)
                     /gene="yceD"
                     /locus_tag="OHO85_002784"
     CDS             complement(21580..22101)
                     /gene="yceD"
                     /locus_tag="OHO85_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCQ2672135.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPVQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22300..22884
                     /gene="yceF"
                     /locus_tag="OHO85_002785"
     CDS             22300..22884
                     /gene="yceF"
                     /locus_tag="OHO85_002785"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCQ2672136.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTL
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(22996..23955)
                     /gene="rluC"
                     /locus_tag="OHO85_002786"
     CDS             complement(22996..23955)
                     /gene="rluC"
                     /locus_tag="OHO85_002786"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCQ2672137.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24528..>24715
                     /locus_tag="OHO85_002787"
     CDS             24528..>24715
                     /locus_tag="OHO85_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672138.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGA"
BASE COUNT         6122 a         6665 c         5964 g         5964 t
ORIGIN      
        1 ataacctttc attcacagtc accaggtaca acggcatcat ggcaactgac tcaacacaat
       61 gtgtaaaaaa aagccgtggc cgcccaaaag tgttcgacag ggatgccgcg cttgataagg
      121 ccatgaaatt gttctggcaa cacggttatg aagcgacttc tcttgcggac ctcgtcgaag
      181 cgaccggagc caaagcgccc acgctatacg cggaatttac caacaaagag gggttatttc
      241 gtgccgttct cgaccgctat atcgatcgtt ttgccgctaa gcatgaagca cagctgtttt
      301 gtgaagagaa aagcgtggag tctgcgctgg ctgactattt tgctgccatc gccaactgct
      361 ttaccagcaa agacaccccg gcaggctgct tcatgatcaa caactgcacc accctctccc
      421 cagattcagg agatatcgcc aatacgttga aatcacgcca tgcgatgcaa gagcgcactt
      481 tgcagcagtt tttatgtcaa cgacaagcgc gcggggaaat cccgacccac tgtgacgtga
      541 cacatctggc agaattcctt aattgtatta ttcaggggat gtcgatcagc gcacgcgaag
      601 gtgcatcgct ggaaaaacta atgcagattg ccggaacgac tttgcgttta tggcccgaac
      661 tggtgaaata agtcgcaaaa aaagcccctc agcctgagcg aatgaggggc caaattgcag
      721 agtacagcct tgttatactt tgttattcaa aatcagttgc ccattactat ctagcgggat
      781 ctgcgtaccc ggatcgcggt ccatgcggat tttgccctgc tgatcgccaa tcttataggt
      841 cacatcataa ccgagcattt tttctgactt gtcatacacc gttttacaac gctgttgcgt
      901 agtcgtgtaa gtatcgcttt cctggagaga gccctggatc tggttaccgg catatccacc
      961 acccagcgcc cccacaacag tggcgacatc tttaccacga ccaccaccaa actgatgccc
     1021 tatcacgccg ccagcaacag cgccgagcac cgacccggta atgcgatttt catcctgcac
     1081 cggtcgacga tgggtcactg tgacgttgcg acactcctga cgcggtgttt taaccgtttc
     1141 cttgattggg gttgcagaaa caacctgagc gtattgcggg ccccgttcaa acacgttcag
     1201 actggccact gccgctacgc ccagcgcagc tgcgacacca atcccgatac ccgccaacat
     1261 tgatttattc acgggaacac ctccttcttt gcagttatgc caatcatgca agcgaaggag
     1321 aaaagcagct atcagacaga agatgaaaag cgcgggaatg gctggaaata cttaaggatt
     1381 cggaggagtc tgaaggaatg cctgatgcga cgcttaacgc gtcttatcag gcctaccgct
     1441 cgtaccattt gtccagggcg gatgaggcgt ttatgccaca tccgccagtg tacgtcgatt
     1501 aatgcaactt caaacgcgga cggataacgc ggttaatact ccccaccagc atcattaatc
     1561 cggttttaaa gtaaccatgc aacgcaatct gatgcattcg gtatagcgag atatatacaa
     1621 agcgcgcaat tcgtccttca atcatcattg agccgcgcgt caggttaccc atcaggctac
     1681 cgacggtgga gaagttcgac agcgatacca gcgaaccatg atctttatac tgataatttt
     1741 tcagcggctt accgttcatc tgcgccagta tgttgttcat tgcgcaagtc gccatctggt
     1801 gtgcagcctg agcacgcggc ggaacaaagc ccccttccgg acgcgggcat gacgcgcagt
     1861 cgccaatagc gtaaatgtct ggatcgcggg tggtttgcag cgtcggttcc accaccagct
     1921 ggttgatacg gttagtttca agaccaccga tatctttcag gaagtctggc gctttgatcc
     1981 cggctgccca caccatcaga tcagcctcaa tatattcgcc atctttagtg tgcaggccgc
     2041 cttcatcagc actggtgacc atggtttgcg tcagcacgcg aacgccaagt ttcgttagct
     2101 cgttgtgggc cgcagcagag atacgtggcg gtaatgcagg caaaatacgt tctcccgctt
     2161 ctaccagcgt tacgttcagg gcttcgttgg tcaggccttt gtaaccgtag ctgtgcagtt
     2221 gcttgaccgc gttgtgcaat tcagcggaga gttctacacc cgtcgcgccg ccgccgacaa
     2281 tcgcaatgtt cactttgcca tttgcgccca ggttggcgga gtatttcagg aagagattca
     2341 gcatctcctg gtgaaagcga cgcgcctggt gcgggttatc gaggaaaatg cagttctctt
     2401 tgacacctgg cgtattgaaa tcgttagagg tgctacccag cgccattacc agggtgtcat
     2461 aggcgatttt acgttccgga accagcagtt cacctttctc atcgcgcagt tctgcaatag
     2521 tgattgtttt cgcttcacga tcaatatcaa tgacggaacc cagctggaac tggaaaccat
     2581 gattgcgcgc atgggccaga tagctcaacg catcgacgcc ttcatcaagc gagccagtcg
     2641 ccacttcgtg cagcagcggt ttccacaggt ggctgtggtt acgatcgacc agcgtaattt
     2701 tggctttttt cttacgtccc agcttatgcc ccagctgtgt tgccatttcc agcccaccag
     2761 caccgccgcc gacaatcaca atctttttca atggcgtagt caacgtgacc cccttaaatt
     2821 tattaaccaa ttgttaatta atggttatta acatagctct taattaacaa caggttacag
     2881 caatgaaaat gttcttcgct attgagaata acatgaatgg tgcattggtc ataccaaaat
     2941 tgatgttaat caagttttgt tgctgaaaag ataggcgatt ttaccgggtg agatcaaaaa
     3001 atagagttta ttgtttatta tttgagcaaa ttcggaagag taatataggg tgaagcgtat
     3061 atcgagggcg tttggtcgtc ctgataagat gcgcagcatc gcatcaggca ttgatgctcg
     3121 actgtcggat gcggcgtgga cgccttatcc gacttacaga attcggagcg catcaggtac
     3181 cggtaaggtt acctgatgcg acgagggcat ttacccgagg gttttgaacg ctttaatgcg
     3241 ctgtaaatgc ggggagatat tcttgaattt gtgcgtctgc tcttcgtccc agacaatctc
     3301 gtaataatga tgcaactctt cagatgtccg ctggctgtta agtgcttcat cattacgcga
     3361 caaaataacc agacaacgat cgcgattctt ctcgcggaag ttggtcacac acttagtggc
     3421 aatatcggca tactcttccg ggcgatcaat cttgccttcc atgttctcat aagggaacaa
     3481 attagggttg aagatcacct ggcggatatc gcaaagaaaa ccaatccgtt ccgcccagta
     3541 tccgcccagg ccaacgccgc aaattagcgg acgctcgtca acgttcagtt gcaacatttt
     3601 gtccacttct ttaagcagat gctgcatatc atgtttcgga tgccgcgtac tgtagcttat
     3661 caagcgtaca tccgggtcaa taaactgcaa ttgtaagact ttctcgtggt tacccggact
     3721 gttagagtca aaaccgtgta aatagataat catcgcttcc tcacataagc cagggttagt
     3781 gacctgcttt ctcttcctgc cagcgttcgt gtaactgatt cagacgggtg ctgatcgctt
     3841 tccagcgagc cgagtccatc agttcctgtc gcgaaaatga acctttatga tacaaacgtg
     3901 taacacgttc tgccttgatc ggtgacagat tatctaacac gctgacggcc cctttacgat
     3961 tattgcagac caggatcata tcgcaacccg catccagtga tgcctgcccg cgttcggcat
     4021 aactgcccat aatcgcggca ccttccatcg ataaatcgtc agagaaaatc acgccgtcaa
     4081 aacccagttc ctgacgcaaa acggttttca gccagtaggg agaaccgctc gccggacgcg
     4141 aatcaacatc actgtagatc acatgcgcag gcataatggc gtcgagttta ttttcgcgga
     4201 ttaaggaact gaagaccgac atatctttag cgcgaatctc cgcttgtgga cgtggatcgc
     4261 acggtgtttc tttgtgtgag tctgccgtta ctgcaccgtg tcctgggaag tgtttcccgg
     4321 tcgttttcat tccggcttca tgcataccat caataaaccg gctggcaatt gccagggctt
     4381 tttgtggatc ggcatgataa gaacgctcgc caatcgccgc gctgatatgc ccgacatcca
     4441 gcactggcgc aaagctgata tcaatatcca tagcgatcat ttcgctggcc atcagccaac
     4501 ccgcctcttg cgccagtttg ccgccctctt ccattcctga cagcgcagcg aatgattgcg
     4561 ccgctggcaa gcgggtaaaa ccttcacgaa aacgctgcac gcgtccacct tcctgatcta
     4621 ccgccaccac cagatgattg cgcgaagctg cgcggatctg gcgcaccagt tcacgtaact
     4681 gggcaggatc atgatagtta cgcgtaaaga gaatcagccc tcccaccagc ggatgcgcaa
     4741 gtatttcacg ctcttccgcg tccagttcgt aacctttgac atccaacatt actggaccca
     4801 cactgctctc cttattgttt tattaatagc tgccgccagg tgtcatcggc cagcctgata
     4861 aattgttgat cgccggtttg tcgccagcgg tactcaaacc accctgcttt gagcatcagc
     4921 agccagggaa accatcgcct gacctgacgc cataattgcg ccggataaat cttcgcgcga
     4981 gtggcatagt cattgaccaa ttgccggtgc tgttcagtat tttccaccca caccgccgcc
     5041 agttccagcg cgatatcacc atctcctgca tactcccagt cgatgagttt taaccctgac
     5101 gcgctatgca ctaaatttcc ggcgtggaca tccatatgca atggacttaa gcgtaaaggc
     5161 cgtggttccc gcgctttgcg cagacgtttt aacattcgca gccaacccac tgtccgccgc
     5221 gccggatcgc tttgctgcca gtacagttcc agtaacggca acagcgttat tcgccagcca
     5281 aaacgtggtt gttgatgtag ataatacagc aagcctgcca gttcgttggt atccggcaaa
     5341 tacgttttta ccgcgccggg cagatagtcg actaccatcc agtcacggag atataaatgc
     5401 ggcttcggtg caatgcatgc gggtagttgt gataaagccc gatactggcg caagaacgcg
     5461 gactgcggcg catcaggatc gtgcggctga cgcacaacaa aacgctggcc ctggtgttca
     5521 atgagaaaac tcccgccact aagcccacta ttaaacgtcg tgacgggatg aaactgcggg
     5581 aaaaagcgcg acagcaattc gtcgcgcgtg atgggattat tgctgcgaaa cggcaccttt
     5641 acctgaccag ataatttcgc ccgtctgcac cagcatcagc tgcatttgta gggtcggagc
     5701 gttaacgttg ccagaggcgc tggagtatag cacgtaatga gcgccgacat tgcgggcaat
     5761 gcctatggct ttactacggg tacctaaact gtcctgcggc gacaaaccta actgttgctt
     5821 agccatcgac agctgctggg cggaaaccag ggtaaattta ccgttattag ccagcgcatt
     5881 tcgcagcgtt tcggtcgctt ctgcggcatt cagcgaaccg ttagtacggt tgttaacgct
     5941 atcaaccagc aggacgctac ccgcagtcac cccgtcagcc ccaagcatct tactgaccat
     6001 cggctgcatt gcgccattcc agtcataatg gcgaatatgc ggcgcaggcg gtgcagtttg
     6061 atcttcgtgc tcaattgggc ctggctgctg cgggatcgtc ggcaccgagg gcactgtggg
     6121 gacaggctgt tgtggctcgg ctggttgttc cggcgctggt ttcacttctt ctaccggtgc
     6181 aggttcacgt tgccccacac acccggcgag aaacatcgcc agcgcggtaa tcaaggcgta
     6241 gcgactcatt tttgtcatca agattcaccc cttacaaata tagataaagt ctgactttgt
     6301 gcgcccccag aaaattggcg ctgccgtaca gcgttaccgc cgaatgtgcg ggaatggtaa
     6361 cgctgcgtgg cctttccaga ggatgcatct ccagccctct ggcgtcatac cagtaaaaac
     6421 gataatgaac ggtaatgggt tcttgccttt cgttataaag cgttgaggat gctgaaggtt
     6481 gaatatcaga cgtcgaaagg acgggctttt ctgcactgat gcccgcagcc aataaagatg
     6541 actccatcac cagcgattgc tcatcattca ccggaatttc cggatgtgaa cgacagccca
     6601 ccagcagtaa caacgccaga gacaccagcc caaagcatcc ttttctcatc gttacagacc
     6661 tttatgcgcc agcattggtc ccagcggacg gccacccaac aagtgcatat ggatgtggta
     6721 aacctcttgt ccgccatggc ggttggtatt catgatcaga cgatagccat cttcggcaat
     6781 accttcttgc tcagcaattt ttgccgctac ggtgatcatg cgtcccagcg cctgctcatg
     6841 ctcagctgac acgtcgttca cagtcggaat gaggatattc ggaatgatca gaatatgcgt
     6901 tggcgcttgc ggcgaaatat cgcgaaacgc cgttaccaga tcatcctggt agacgatatc
     6961 ggaggggatc tcacgacgaa taattttgct gaatatagtt tcttctgcca cgacattttc
     7021 ctttttcaaa tagccatgcg ttgcgccatg ctacgccgcg ccacactgcg agtatagagt
     7081 atggtcgagt taaacctgct ctttcaacct taactcacga tttcttaagc aaaaaatcgt
     7141 tttgctgccg tatatatcgc cattattccc atttctgcct gcgaatgcgc tgaaaagaca
     7201 ttctctgttt ggtttacacg acaaatatga atacgtatat ttctcatttg catttacaag
     7261 caaaattatt cgcacactaa atataaccgc tcatcaccct cctccggcat gagcctgacg
     7321 acaacataaa ccaagagatt tcaaatgctt tcaacacaat ttaacaggga taatcaatat
     7381 caagccatca ccaaaccctc actacttgcc ggttgcatag cattggcact attaccttct
     7441 gccgcttttg ctgcaccagc cactgaagaa acggtgattg ttgagggttc agccacagct
     7501 ccagatgatg gcgaaaatga ttacagcgta acgtctacct ctgcgggtac caaaatgcag
     7561 atgactcaac gtgatattcc tcagtcggtc actattgtta gccagcagcg gatggaagat
     7621 cagcagttac aaacgctggg cgaagtgatg gaaaacacgc tggggatcag caaaagtcag
     7681 gcggattccg atcgtgctct ttattattcc cgcggattcc agatcgataa ctatatggtt
     7741 gatggtatcc ccacctattt tgaatcgcgc tggaatctgg gcgacgcact ttctgatatg
     7801 gcactgtttg aacgcgtaga agtagtgcgt ggcgcgacag gactcatgac cgggacgggt
     7861 aatccatctg cggcaattaa tatggttcga aaacacgcga ccagtcgtga atttaaaggc
     7921 gatgtctcgg cggaatacgg tagctggaac aaagaacggt atgtggcgga tttacaaagc
     7981 ccactcaccg aagacggtaa aatccgcgcg cgaattgtcg gcggctacca gaataacgac
     8041 tcatggctgg accgctacaa cagtgaaaag accttcttct cgggcattgt cgatgctgat
     8101 ttaggcgatc ttacgacgct ttcagccggt tacgaatatc agcgcattga tgttaatagc
     8161 cctacctggg gcggtttacc gcgctggaat actgatggca gcagcaacag ttacgatcgt
     8221 gcacgcagta ccgcacctga ctgggcgtac aacgataaag agatcaacaa ggtctttatg
     8281 accctgaagc agcggtttgc tgatacctgg caagcgacac tgaatgccac ccactctgaa
     8341 gtcgaatttg acagcaaaat gatgtatgtc gatgcctatg taaacaaagc ggatggtatg
     8401 ctggttgggc catacagtaa ttatggacct ggctttgatt atgtcggcgg caccggttgg
     8461 aacagtggca aacgtaaagt tgatgcgctg gatttgttcg ctgacggtat ttatgaattg
     8521 tttggtcgtc agcacaatct aatgtttggt ggcagttaca gcaaacaaaa caattgttac
     8581 ttcagttcat gggccaacat cttcccggat gaaattggca gtttctacaa ctttaatggc
     8641 aatttcccac aaaccgactg gtcaccacag agcctggcac aggacgatac cacacatatg
     8701 aaatcgttat atgctgccac tcgagtcacc cttgccgatc cgctgcatct gatcctcggc
     8761 gcacgttata ccaactggcg ggttgatacg ctgacttaca gcatggagaa aaaccacacc
     8821 acgccttacg ctggtctggt gtttgacatc aatgacaact ggtcgaccta cgccagctat
     8881 acctctattt tccagccgca aaatgatcgt gacagttcag gcaaatatct ggctccaatc
     8941 accggtaaca actacgagct gggtctgaaa tcggactgga tgaatagccg tctgaccacc
     9001 acgttagcca tcttccgtat tgagcaggat aatgtcgctc agtccaccgg tacacctatc
     9061 cccggcagca acggcgaaac cgcctataaa gcggtggatg ggacagtcag taaaggggtg
     9121 gaatttgaac tcaacggcgc aattaccgac aactggcagc tgacatttgg cgcaacgcgc
     9181 tatattgcag aggataacga aggaaacgcc gttaatccta atctgccacg caccacggtt
     9241 aaaatgttca ccagctatcg gttgcctgtc atgccagagt tgacagtcgg cggtggtgtt
     9301 aactggcaaa atcgcgtgta taccgacacc gtgacaccgt atggcacctt ccgcgccgag
     9361 caaggtagct acgcgctggt ggatctcttc acccgctacc aggtgacgaa aaacttctcg
     9421 ttacagggga cgtcaataac ctgttcgaca aaacctacga taccaacgtg gaaggttcta
     9481 tcgtctacgg cgcaccgcgt aatttcagca ttaccggcac gtatcaattc tgaataacac
     9541 ctgtaaaaaa ggcagccatc tggctgcctt agtctcccca acgtcttaca gattagtggt
     9601 tacggatgta ctcatccatc tcggttttca ggttgtcgga tttagtaccg aaaatcgcct
     9661 gaacaccaga accagcaacc actacgcccg ctgcgcccag tttcttcagg ccagcctgat
     9721 ccactttaga cacatcagca acgctgacgc gcagacgggt aatacatgcg tcgaggttag
     9781 taatgttttc tttaccacca aatgcagcaa ccagagccgg tgccatttcg ctggtacctg
     9841 tcgcttttgc atcttcagtc gcgtcttcac gacccggcgt tttcagatcc agtgctttaa
     9901 tcagcacgcg gaagatggtg tagtaaacaa tcgcataacc gataccgacg atcgggaaca
     9961 gccacagttt gctgctgtta ccagacagaa cgatgaagtc gatcagaccg tgcgagaacg
    10021 acgtaccgtc acgcatcccc agaagaatac agattgggaa tgccaggcct gccagaatcg
    10081 cgtggatgat gtacaggatc ggcgcaacga acatgaagga gaactcgatc ggctcggtga
    10141 taccggtcag gaacgaggtc agcgccgcgg agatcataat accgcccact ttcgcgcggt
    10201 tttctggttt agcagagtgc cagatagcaa ttgcggcagc tggcagaccg tacattttga
    10261 acaggaagcc accagacagt ttacccgcag tcgggtcacc cgccatataa cgcggaatgt
    10321 cgccgtggaa aacctgacct gctgcgttgg tgtattcacc aatctgcatc tggaaaggta
    10381 cgttccagat gtggtgcaga ccaaacggta ccaggcaacg ttcgatgaaa ccgtaaatgc
    10441 caaacgcaac taccgggttc tggtaagcag cccactgaga gaaggtctgg attgcagaac
    10501 caatcggcgg ccaaatgaag gacagcacaa cgccagtaaa gatggcagcc aggccagaaa
    10561 tgatcggcac aaagcgttta ccggcaaaga agccaagata ctcaggcagc ttaatacggt
    10621 agaaacggtt aaacatgtac gctgcgatcg caccggagat aatccctccg agtacgccag
    10681 tatccgccag gtgtttagag gcgatttctt cagcaggtaa atgcagtacc agtggcgcaa
    10741 ccacggccat ggttttaacc atgatgccat aggcaacaac tgcggccagc gcggatacgc
    10801 catcgttatt ggtaaagccg agggcgacac cgatcgcaaa aatcagtggc atgtttgcaa
    10861 agacggaacc gcctgcttct gccataacat gcgatacaac ggcgggcagc cagctgaaat
    10921 tcgcggaacc gacgcccagc agaatacctg cgataggcag tacggatacc ggcagcatca
    10981 gcgatttacc gaccttttgc aggttagcaa atgcattctt aaacataatt gagagtgctc
    11041 ctgagtatgg gtgctttttt tacgttctca cgcgtggcaa ggggggagag cctcgccgtg
    11101 tacagggcat ctaagcgccc tttatttatt acacagagta aaataattca gtgccaattt
    11161 gtttgacggc tatcacgttt caacttcaga tcattggaga agttgcacat tttgacaaaa
    11221 catatgtcac aatatttcaa acgtggttat tcaccgccca attttcggcg gtgaacttta
    11281 ctcgttttag ccattaatta cgagctttaa aaaaactcat tcaacggatg gattgaaggc
    11341 gggaagagtc gatgtgaaac agacgggcga agttatcggt ggttacctgc gccagttctt
    11401 caacggcaac acctttcaac acagccatgt attctgcaac gtcacgaacc atcgcaggtt
    11461 gattctcttt tcctcgatgc ggtaccggcg caaggtaagg tgagtcagtt tccaccagta
    11521 accgatccag ggggacataa cgcgcagcat cgcgcagttg ctccgcatta cggaaggtca
    11581 caatgccgga aaaggagatg taaaatccga gatccagtaa tttgcccgcc gtttctctgt
    11641 cctctgtaaa acagtgtagt acgccaccgc aatccgtcac tttttcttcg cgcagaattg
    11701 ccagcgtatc ggcgcgggcg tcacgggtat ggacgataac cggcttgttc agttcacgac
    11761 caatctggat atgatggatg aaggactctt gctgacgaac tttagtttcc ggtgtgtaat
    11821 aataatccag cccggtttct cccagcgcta caacaccttc ttctgccgcc agacggcgta
    11881 aatcttctac atcgtaggga tcattctggt tgagtggatg cacgccacaa gaaaatacga
    11941 cgttgtcacg ttcgcctacc agataccgca tgtgtaaata gcccggtaat gttgtggcga
    12001 ctgccagaca aaaattcaca tcgcgtgcgg cggctttcgc cagaacgtca tccacgtcct
    12061 tatgcaaaga ttcataatcc agaccatcga gatggcagtg tgagtcgact aaaaacatga
    12121 tgtctctctt aaagatgagg aaccggtagc acaacgcccg gttgcaggta atgctcaata
    12181 cgcagtaaaa gatcggtgat gagaagctcg cggttgatgc ctgtgacaga cattaactgt
    12241 tcacgaatgt ggcaaacatc ccccagtata gcctgcaggc gcgagggaga aagatggttt
    12301 gccagttcga cgacaaggcc cggcacatca acattggtca cctgcgcagc accatgatgg
    12361 cgttttagcg catccatcag caacgttgcc agccagtgta aacgcgccgg agcttgttca
    12421 tgattaaggg ccgccagcag cgaataccag tcgcccgatg gcacgctata tgccaatgcc
    12481 tgacacaatg tttcacgagc ctgccagtta tctccctgaa acaacgccag tgccgcgcca
    12541 ggcgaaccgg cgcttaagcg caatgcggca agtaatgcat cctgtgacat tgtcacttcg
    12601 cgtgaaagcc aggtcacggc gtactgttcc ggcggcggcg caaggtaatg tagccgacaa
    12661 cgactacgta atgttgccag taaacgttca ggctcgcggg tagccaggaa aaaccaggtt
    12721 tctgctggtg gctcttcaag cgttttcagc aatgcgttag ccgcggcgtc ggtcagtaag
    12781 gcagcatcgg ttacccagac aactttcgca ccacctaagc gtgcgtgctc attcagcttt
    12841 tcggtgacct cacgtaccgc atcaatgccc aaagcatttt tccctttttc gggagccagg
    12901 gtgtagtaat cgggatgcgt gccagcctgc atcaactgac atccacgaca gtgaccgcaa
    12961 cttttgtgac cctgcggttg ttggcagagt aaataacggc tcagggcgta gattaaagca
    13021 tcatcgccca tgcccggtaa cgcctgaatg agcagcgcat ggtgacctct tccggcctga
    13081 tagctggcta ccagtttttc gaaatcaggt cgtaaccatg gataccatct catgcgtcca
    13141 actccttcac ccagtgggtc acggtagtgc ggattgcatc catcacggcc tccagcggct
    13201 gggtggcatc aatggtatga atgcttttat cttgtgctgc cagttccaga tagcgggcgc
    13261 gggtgcgatt aaagaaatcg aaagactctt gctcaatacg atccagctcg ccgcgcgcac
    13321 gcgcgcgttt taagccaact tccggggtaa catcgagata gagcgttaag tcggggcgaa
    13381 aatccccgag aacagcatca cgcagtgttg ccagcatatg ttggtcaata ccacgtcctc
    13441 cgccctgata cgcctgagtg gagagatcgt ggcgatcgcc aatcacccag gtgccgttag
    13501 ccagcgcagg tttgatgact gtttctacca gttgaacgcg cgcggcataa aacatcagaa
    13561 cttcggcttt atcggtaatg acttcatcgc ctaccgattt gatatccagc accaggcttc
    13621 ttaacttttc ggcaagttgc gtaccgccag gttcccgagt gaaaaccatg tcgcggatac
    13681 ccagttgctc gagcgtctca accaccacat tacgcgcggt agttttgcct gcgccttcca
    13741 gcccctcaat gacgatatac ttactgcgca tttttttcct taagcacttt cagataatcc
    13801 tgcacagact tgttatgact ggcaagattg gtattaaacg tgtgaccacc tttaccatcg
    13861 gccacaaaat agagatacgg cgtttttgcc ggatgcgcag cagccttcag cgaatccgcc
    13921 cccggcgtag ctatcgcacc cggcggcaaa ccggtaatgg tataggtgtt atacgctgtc
    13981 ggcgtttcca ggtctgcacg agaaagtttg ccattataac gctctcccat cccgtaaatc
    14041 acggttgggt cggtctgcaa gcgcatacca atgcgtaaac ggttgataaa taccgaggca
    14101 acctgatcgc gttcactggc aacggcggtt tctttttcga taattgatgc catcgtcacc
    14161 agctgatttt tatctttata aggcagaccg tccgcacgcc cttcccaggc gctatcgacc
    14221 gctttcacca ttttcttgtg cgctcgcttg agtaacgcga catcggtggt attggcggta
    14281 tacatccagg tgtctggcca gaaccaacct tcaatccact ccgggttttc cagttcaagt
    14341 gcctgcgcta cggtggcgta cttatcgtcg ctcagcgtat gcttgatata cggggcctca
    14401 cgcaattgct tgaggtaatc gctcagacgc atcccttcta ccagtcgcag agggaactgt
    14461 gcttctttac cgctttccag caacttcagc atctcgcgca cggtcatctg cggtgtaaag
    14521 cggtaagtcc cggctttaaa gtgagaaaga tccggttcga tacgcagcag ccattgaaaa
    14581 acccgtgggc gattgatgat cttatcggca taaagctgtt caccgagcgc cagacgtccg
    14641 gtccctggct tcagggtaaa tatcgtctct tctttgataa gcaatttgct gtcggcaaga
    14701 tggcgaacct tccagacgcc cacaccagcg gcgataccca gtaccaccag caataacaag
    14761 ataatcaata acactttttt catgactaat tcgggcgctc acaaagtggg gctaaatatt
    14821 catataacgt tgctgacgaa aaggagacat cgccacaggc acgtacgggc atcactggca
    14881 ttaacgcatt acaaataacc atctcatctg cctgcaacgc ctcttccaga gaggcttgca
    14941 cttcgacaag ctgataagag gattgtgcca gcaaacggat acagaattgt cgcataatgc
    15001 cgttaacacc tgcctgatcc agtcgcggcg tataaactac attgcccttc cgccagaaca
    15061 aattagccgc acagcattcc gtaacccacc cttcgctgtc aaggaccagc gcctcatcag
    15121 cgtttgtctg ctcaagatga gagcgaatca atacttgctc aagtcgatta agatgtttaa
    15181 tacctgcaag atgaggattg cgccccagcc gcaccgggct tagcgccaac gtcatcccct
    15241 cgttacgcaa acggtcgtaa tgtgcaggat aagccgtaac ggagagaatc cgcgttgccg
    15301 gtccactgtt caatgtgctg taccctcgcc cgccactacc gcgactgatc acgactttca
    15361 gtacaccatt ttgctgttct gctgccagcg ttttcatctc ttgttcaagc tgaggccaga
    15421 agtcacagga aatcatcaac cgctgacaag catcatgtag tcgctggata tgcgcagata
    15481 ataaactgac tttaccgtcg ataactctgg cggtggtaaa acaaccatca ccaaactgcg
    15541 ttgcccgatc gcttactgcc agcgattcct gcttataacc gttaattaag aacataccag
    15601 ctccttatca gagaattgtt agtgtggcag catgttcact acggaacaag tcggaataaa
    15661 agctaagaaa aaaggcccgc aagcggacct tttataaggg tggaaaatga caacttagat
    15721 ctttttaaag atcaaagaac cattagtgcc accgaagccg aaggagttac acagcgtgta
    15781 ttccattccg ctgacctgac gtgcttcatg cggtacgaaa tccagatcgc aaccttcatc
    15841 cgggttatcc aggttgatgg ttggcggaac agcctgatcg cgcagcgcca ggatggagta
    15901 gatagattct actgcacccg ccgcacctaa caggtgaccg gtcatagatt tcgtggagct
    15961 taccaacaca cggcttgcag cttcaccgaa gatggttttc accgcctgcg cttcagcttt
    16021 atcgccagcc ggcgtagaag taccgtgcgc gttaacgtag ccaatctgac tcgcttcaat
    16081 gcctgcatca cgcagagcat ttgccattgc cagagctgcg cctgcgccat tttctggcgg
    16141 tgacgtcata tgataagcat cgctgctcat accaaagccg acgagttcag cgtaaatttt
    16201 cgcaccgcgt tttttcgcgt gttcgtactc ttcaagtacc agcataccgg caccatcgcc
    16261 cagtacgaag ccatcacgct ctttatccca cgggcggctc gccgcttgcg ggttatcatt
    16321 gcgggtagat aatgcacgtg ccgcgccaaa accaccaacg cccagcggcg tactggcttt
    16381 ctctgcgcca cctgcaacca tcacgtcagc atcgccatac gcgataatac gcgcagcatg
    16441 gccaatgttg tgcacgccgg aagtacaggc ggtcgcgata gagatgcttg ggccacgcag
    16501 gccatacatg atagtcagat gacctgccac catgttcaca atcgttgacg gaacgaagaa
    16561 tgggctgatc ttacgtggac caccgttcat cagagatgtg tggttttctt cgatcagtcc
    16621 gaggccgcca atcccggagc caattgcggc accaatgcgg gttgcgttct cttccgttat
    16681 ttcaaggcca gaatcctgca tggcctgaac gccagcgaca attccatatt gaatgaaggc
    16741 atccatcttg cgctgttctt tgcgcgagat aatgtcctca cagttaaaat cctttactaa
    16801 gccagcaaat ttcgttgcat aggcgctagt atcgaaatgg tcgattaggc tgatgccact
    16861 ctgaccggca agcagagctt tccaggtaga ctctacggta ttgccgacag gagacaacat
    16921 gcccagtccg gtcacaacta cacgacgctt agacacgttt gtcctccagg gagggaaaaa
    16981 atgattctag tgggacaaaa agataaaact caggcggtcg aacgaccgcc tggagatgtt
    17041 cacttacgcc tggtggccgt tgatgtaatc aatggcagcc tgaacggtgg tgattttctc
    17101 agcttcttcg tccggaatct cagtatcaaa ctcttcttcc agagccatta ccagctcaac
    17161 ggtgtcaaga gaatccgcgc ccaggtcttc aacgaaagaa gcattgttgg taacttcttc
    17221 ctgcttaacg cccagctgtt cgccgataat tttcttaacg cgttcttcga tagtgctcat
    17281 actcttaaat ttcctatcaa aactcgcttt cgcgatggtt ttcgtagtgt ataaaatgtt
    17341 gaaaaatttg caactaaatc ccggcaggtc ttaccacgat tttacgttat tttgaggcct
    17401 taccccccaa taacgcaaat cattttgcac taattattac tcataaccac gcagactgcg
    17461 cgcaacatga gcattttgtg caaatcgcgg tcagaccatg tacatcccgc cgttcacatg
    17521 caaagtttca cccgtgatgt aagctgcttc gtcggatgcc aggaatgcaa ccgcgttggc
    17581 gatttcctgt gcgccgccga ggcgacccgc aggaacctgc gccaggatac ccgcacgctg
    17641 gtcatcgctc agcgcacgtg tcatgtccgt ttcaataaag cccggagcaa caacgtttac
    17701 agtaataccg cgtgacgcaa cttcgcgcgc cagtgattta ctgaagccga tcaagcccgc
    17761 tttcgccgca gcgtagttgg cctgaccgcc atttcccatg gtaccaacca cagaaccgat
    17821 agtgataata cgaccatgac gctttttcat catagcgcgc attaccgctt ttgacagacg
    17881 gaaaacagat gaaaggttgg tttcgataat atcgttccac tcttcatctt tcattcgcat
    17941 taacaggtta tcacgagtga taccggcatt attgaccagg atatccactt caccaaattc
    18001 cgcgcgaatt ttttccagaa cagattcgat agatgccggg tcggtcacat tcaacatcag
    18061 acctttgccg ttagcaccta aataatcact gatcgcctga gcgccatttt cactggtcgc
    18121 agtgccaata actttcgcgc cacgggctgc gagcgtttca gcaattgcgc ggccaattcc
    18181 gcggcttgca ccggttacca gtgcgatttt tccttcaaaa ttcatgattt tcctctttta
    18241 aagctcgagc gccgctgcca tcgctgaagg ttcgttcagc gccgaggcgg tcagggtgtc
    18301 gacaatgcgt ttcgtcaggc cagtaagcac tttgcccggg ccgacttcat agagatgttc
    18361 tacgccttgc gctgccatgt actcaacaga cttcgtccac tgaaccgggt tatacaactg
    18421 acgtaccagt gcgtcacgga tggcatcacc attggtttcg catttcacat caacgttatt
    18481 cacaacagga actgttggtg cgttaaaggt gattttcgct aattctactg ccagtttgtc
    18541 ggctgctggt ttcatcagcg cacagtgaga cggtacgctc actggtaacg gcagagcgcg
    18601 ttttgcgccc gcagctttac aggcagcgcc agcacgctca accgcttctt tatgaccggc
    18661 aataaccacc tgtcccggag agttaaagtt taccggagaa acgacctgac cttctgcagc
    18721 ttcttcacac gctttcgcaa tagacgcatc atccagaccg atgattgccg ccatagcgcc
    18781 cgtgccttcc ggtacggctt cttgcatgaa cttgccgcgc atctcaacca gacgcaccgc
    18841 atcagcgaaa tcaatcacac cagcgcaaac cagcgcggag tattccccca ggctgtgacc
    18901 ggccatcatt gccggtgctt taccgccctg ctgctgccat acgcggtaca gcgcaacaga
    18961 tgcagtcaac agcgctggct gggtttgcca ggttttattc agttcttcag ctggcccctg
    19021 ctgggtcagc gcccacaggt cgtagcccag cgccgcagaa gcttcagcaa acgtttcttc
    19081 gacaattgga tagctcgccg ccatatcagc cagcattcca acggtttgag aaccctgtcc
    19141 agggaacaca aatgcaaatt gcgtcatgtt ttaatcctta tcctagaaac gaaccagcgc
    19201 ggagccccag gtgaatccac cgccaaaggc ttcaagcaga accaactgcc ccggcttaat
    19261 gcgcccgtcg cgtacagctt catccagcgc gcacgggaca gaggccgcag aggtattacc
    19321 gtggcgatcc agcgtcacca cgacattgtc catagacata ccgagttttt tcgccgttgc
    19381 actgataata cgcaggttag cctgatgcgg aaccagccag tccagttgag aacggtcaag
    19441 attatttgcc gccagcgtct catcaacgat gtgcgccagt tctgttaccg caaccttgaa
    19501 gacttcgttg cccgccatcg tcagatgaat tgaattctct ggattcacac ggtcagcatt
    19561 aggcagcgtc agcaactcac catagctacc gtcggcatgc agatgggtgg agatgattcc
    19621 cggctcttca gaggcagcca gcaccgcagc gcccgcgcca tcgccaaaaa taataatagt
    19681 cccacgatcg gttggatcgc aggtgcgcgc cagtacatcg gaaccgacga ccagagcata
    19741 cttcaccgcc ccagatttca cgtattgatc ggctacgctt aatgcatagg tgaaacctgc
    19801 gcaggctgct gcaacgtcaa atgccgggca acctttaatg cccagcatgc tttgaatctg
    19861 acaagctgcg ctcgggaaag cgtgcgtagc agaagtcgtt gccacaacga tcaggccaat
    19921 ctggtctttc tcaatgcccg ccatctcaat tgcgcgtgtc gccgcttcaa agcccatggt
    19981 tgaaacggtt tcgtttggca cggcaatgtg gcgttcgcgg ataccggtac gagtgacaat
    20041 ccactcgtca gaggtgtcca ccattttttc caaatcggcg tttgtccgca cttgttcggg
    20101 cagatagctg ccagtaccaa taatcttcgt atacatgtac gctcagtcac ttttcggtta
    20161 tataccgtca cttgcaaact gcgagttcgc tggcagcgtc ctgctaccgc agagttccgc
    20221 ttttgccacc gtccagcagc tcaaaaccag ctgggtatac agattccagg cgagcggcaa
    20281 ttcgctgagg aacttgtcgc tgcaccgcct gcactgcctg ttcaatcgcg accgcaaaag
    20341 ctcgctgatt ggctgcacca tgacttttta tcaccgtgcc gcgcaatcct aacagacagg
    20401 cgccgttata ctggtcgggg ttgaggtgac tgaatcgcct cgtcaggctc ttttgtagcc
    20461 aacgctttaa taacagtagc caccacgacc gttttttccc ttcaccctga gatttcagca
    20521 gagaaaggaa catcctgaca acaccctcca tcgtctttaa tgtgacattt cctgtaaagc
    20581 cgtcacaaac cagcacatct gtcttgccag ttaacaactc attggcttca agatagccga
    20641 tataattgat agaagggatt gttttaagca ccgctgaggc atcccgaata ctgtcgagac
    20701 cctttacttc ttcttcacca atattgagca acgccacgcg aggattggga atttccacca
    20761 cctcttcagc cagaactgag cccataatgg caaattgcac caacattgta ctgtcacaat
    20821 cgacgttggc ccctaagtca aggaccaccg ttttgccctt ttgctgatgt ggtaataccg
    20881 tcaccagcgc cggacgctca atcccctcca ggggcttgag taataatttt gccagcccca
    20941 tcagtgcccc ggtattaccg gcactgacac aggcttgcgc tcgaccttct ttcaccagct
    21001 ccagggccac gcgcattgaa ctcccacgac tggcgcggat agcttgcgaa ggccgggcat
    21061 cactggcgat aactgactgc gcaggaataa tctgcagacg cgaacgttgt tcaaagtcag
    21121 ctttagcaag taatggcgtg atggcgtcgg gattgccgac taaaagaaga gtgagttgcg
    21181 aattagaatt cagtgcctgc aatgctgcag gcactgtcac ggaagggcca aaatcccctc
    21241 ccatgacatc taacgccagg gttagacgtg tcaaggtatc gtcgccgccc ggtttggtct
    21301 ttccccagtt gcctggggaa aatcctcact aagcttcatc acgcagacgc gtgattactt
    21361 agcgatgacc ttgcggccgc ggtagtaacc gtcggcagtg atgtggtgac gcaggtgttt
    21421 ttcaccagaa gttttgtcta cagacaggct ggtgactgcg gtcagcgcgt catgggaacg
    21481 acgcatgcca cgtttggaac gggttggttt attctgttgt acggccatgg accttactcc
    21541 tcaattacgg tttatccccg atccaacggg gagcaccaat tacttacgct ttaagctggc
    21601 taatacggca aatgggtttg gcttttgcgc ttcttcaggc agttcaccaa agaccatgtc
    21661 cgcttcggac acttcacagt gttcagaatc atgcaccgga actaccggca aggcgaggat
    21721 gatttcatct tcaaccattg caagcagatc gatttcaccg aattcgttaa cctcaatcgg
    21781 ttcatacgct tccggcagtg cttcagcctg ttcgtctgaa cgcacaggac taaaacaata
    21841 cgttgtgtag acctgatgag taaacggctt cccgcaacgc tgacactcga gcgttaccgt
    21901 caccttcgca tcgccgttta acactgcgag acgttggtta tcgatagcga acgacatgga
    21961 gcattccaca tcactgtcca cactgactac ggattcggcg acgcgctcaa cctgaacagg
    22021 ggtatagata ccctggtaat caaggcgttt ttgagccgta cgaaccggat cgagagtcag
    22081 gggtaatttt accttttgca tagggcgcgc atattaactt tgtaacgtca tagagtcaaa
    22141 gaaaaaggca gcctgtggtt gccttttgcc aataattcgc acacattgcg ggttactgct
    22201 ttattttcgt tcagtgctga taacgaacat caacctgcaa aacgaagcgc atagtttaaa
    22261 atggctatca tcaatacgct atatctgatg gaaaaaaata tgcctaaact tattttagcc
    22321 tccacctcgc catggcgtcg cgctcttctg gaaaaactgc aaatctcttt tgaatgtgca
    22381 gcaccagagg tcgacgaaac cccacgcagc gacgaatcac cgcgacagtt ggtgcttcga
    22441 ctggcacaag aaaaagcgca atctctggcg tcacgttatc cggatcattt aattattggt
    22501 tcagaccagg tatgtgttct tgatggcgaa atcaccggta aaccgttaac ggaagaaaat
    22561 gcccgtctgc aattacgcaa agccagcggc aatatcgtca ccctctatac tggactggcg
    22621 ttgtttaatt cggcgaatgg gcatctgcaa acagaagtgg agccttttga cgtccatttc
    22681 cgtcacctga gcgaggcgga gattgataat tacgtgcgta aagagcatcc cctgcactgc
    22741 gcgggtagct ttaagagtga aggatttggc attacgctgt ttgagcgttt agaggggcgt
    22801 gatcctaaca cgctggttgg tttaccgcta atcgcgctgt gtcagatgtt acgtcgggaa
    22861 gggaaaaacc cgctgatggg atagtttttt gcgccggatg tggttacgtc gcatccggca
    22921 atcggtgctg gcggcctgat gcggcgtaaa cgccttatca ggccaaccgc ccattatcag
    22981 gtttatatgc ttgtattagc gcgcgttacg cagcttttgc aaacaacgct tcaaaccttc
    23041 atccatcgga gcttcgatac gcatcacctc accggtcccc ggatgagtaa acttcaacgc
    23101 tgcagcgtgc aggaacaggc gatttaatcc cgtgcctgct tcagtgagct gcctgtcaaa
    23161 ttcacggtca ccgtagcgat cgtcaaaggc aatcggatga cccgcatatt gtgtatgcac
    23221 acggatctgg tgagtacgcc ctgttaccgg gctacaacgc accagggtgg caaatgcata
    23281 gcgttcttcc actttaaagc gtgtttccga cggtttgcct tcctgactca cacgcacgat
    23341 acgttcgccg ctttgcagaa tatttttcag taacggtgct tgaacgctct tcacatgcga
    23401 ctgccactga ccgcgcacca gcgccaggta atctttttgc atcccttttt cacgtaattg
    23461 ctcatgcaga gaacgcaacg ccgagcgttt tttcgctacc agcaaaacac ctgaggtgtc
    23521 ccggtcaaga cgatgaacca gttcaaggaa ccgcgcttcc gggcgcaacg cccgcaaacc
    23581 ttcaataacg ccgaagctta aaccactgcc gccatgtacc gccgtaccgg aaggtttatt
    23641 cagcaccagg atgtgatcat cttcatataa gatgacgtcc gccagcgccg ccaccttttg
    23701 cagatgtggc gaaaccgcct cttcttcccg ctcagcaacg cgaaccggtg gaatacgcac
    23761 ctcatcaccc gcttcgagtt tatattcagg cttaatacgt tttttgttca cccgcacttc
    23821 gcctttacgc aaaatacggt aaatcatact ttttggtacg cctttcaatt gggtacgcaa
    23881 aaagttatcg atacgttgcc ccgcttcgtc agcggtgata gcaacaattt ttacggatgg
    23941 agtctctgtt ttcatggttg gcgattctaa ttagccaaca ggattcgcgc cactcatttt
    24001 tctatgctta tatttacttt tgcaccttat tacttcactg cgtgatcact ttattgatgg
    24061 ttattaaacc aatcaccagc aagaagtgaa aaaactgtga gtaagcgggt gataaatggt
    24121 aaaagtcatc ttgctataac aaggcttgca gtggaataat gaggccgttt ccgtgtccat
    24181 ccttgttaaa acaagaaatt ttacggaata acccattttg cccgaccgat catccacgca
    24241 gcaatggcgt aagacgtatt gatctttcag gcagttagcg ggctgcgggt tgcagtcctt
    24301 accggtagat ggaaatattt ctggagagta atacccagtc tgtttctttg ataattgcgc
    24361 tgtttttccg catgaaaaac gggcaaccga cactctgcgc ctctttgagc tgacgataac
    24421 cgtgaggttg gcgacgcgac tagacacgag gccatcggtt cacacccgga aaggcgttac
    24481 tttgcccgca gcttagtcgt caatgtaaga ataatgagta agttacgatg aaaagaatgt
    24541 taatcaacgc aactcagcag gaagagttgc gcgttgccct tgtagatggg cagcgtctgt
    24601 atgacctgga tatcgaaagt ccagggcacg agcagaaaaa ggcaaacatc tacaaaggta
    24661 aaatcacccg cattgaaccg agtctggaag ctgcttttgt tgattacggc gctga
//
LOCUS       DAJOWB010000064        24600 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJOWB010000064 DAJOWB010000000
VERSION     DAJOWB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.064"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            456..1595
                     /gene="wza"
                     /locus_tag="OHO85_002788"
     CDS             456..1595
                     /gene="wza"
                     /locus_tag="OHO85_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCQ2672139.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1601..2044
                     /gene="wzb"
                     /locus_tag="OHO85_002789"
     CDS             1601..2044
                     /gene="wzb"
                     /locus_tag="OHO85_002789"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCQ2672140.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            2047..4209
                     /gene="wzc"
                     /locus_tag="OHO85_002790"
     CDS             2047..4209
                     /gene="wzc"
                     /locus_tag="OHO85_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCQ2672141.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4387..5226
                     /gene="wcaA"
                     /locus_tag="OHO85_002791"
     CDS             4387..5226
                     /gene="wcaA"
                     /locus_tag="OHO85_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCQ2672142.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5229..5717
                     /gene="wcaB"
                     /locus_tag="OHO85_002792"
     CDS             5229..5717
                     /gene="wcaB"
                     /locus_tag="OHO85_002792"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCQ2672143.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNIWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5714..6931
                     /gene="wcaC"
                     /locus_tag="OHO85_002793"
     CDS             5714..6931
                     /gene="wcaC"
                     /locus_tag="OHO85_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCQ2672144.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIVLFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6906..8123
                     /gene="wcaD"
                     /locus_tag="OHO85_002794"
     CDS             6906..8123
                     /gene="wcaD"
                     /locus_tag="OHO85_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCQ2672145.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8134..8880
                     /gene="wcaE"
                     /locus_tag="OHO85_002795"
     CDS             8134..8880
                     /gene="wcaE"
                     /locus_tag="OHO85_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCQ2672146.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNSIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8896..9444
                     /gene="wcaF"
                     /locus_tag="OHO85_002796"
     CDS             8896..9444
                     /gene="wcaF"
                     /locus_tag="OHO85_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCQ2672147.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9470..10591
                     /gene="gmd"
                     /locus_tag="OHO85_002797"
     CDS             9470..10591
                     /gene="gmd"
                     /locus_tag="OHO85_002797"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCQ2672148.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10594..11559
                     /gene="fcl"
                     /locus_tag="OHO85_002798"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10594..11559
                     /gene="fcl"
                     /locus_tag="OHO85_002798"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCQ2672149.1"
                     /translation="MSKQRVFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASESIDLVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVDYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENRDRFRG"
     gene            11562..12041
                     /gene="gmm"
                     /locus_tag="OHO85_002799"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11562..12041
                     /gene="gmm"
                     /locus_tag="OHO85_002799"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCQ2672150.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIAENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            12038..13261
                     /gene="wcaI"
                     /locus_tag="OHO85_002800"
     CDS             12038..13261
                     /gene="wcaI"
                     /locus_tag="OHO85_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCQ2672151.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCEIFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13264..14700
                     /gene="cpsB"
                     /locus_tag="OHO85_002801"
     CDS             13264..14700
                     /gene="cpsB"
                     /locus_tag="OHO85_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCQ2672152.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPEIDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEMAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEQAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14981..16351
                     /gene="cpsG"
                     /locus_tag="OHO85_002802"
                     /gene_synonym="manB"
     CDS             14981..16351
                     /gene="cpsG"
                     /locus_tag="OHO85_002802"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCQ2672153.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQITLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKEKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LALLNE"
     gene            16406..17800
                     /gene="wcaJ"
                     /locus_tag="OHO85_002803"
     CDS             16406..17800
                     /gene="wcaJ"
                     /locus_tag="OHO85_002803"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCQ2672154.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLPF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17802..19280
                     /gene="wzxC"
                     /locus_tag="OHO85_002804"
     CDS             17802..19280
                     /gene="wzxC"
                     /locus_tag="OHO85_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCQ2672155.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLITNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRINFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19556..20836
                     /gene="wcaK"
                     /locus_tag="OHO85_002805"
     CDS             19556..20836
                     /gene="wcaK"
                     /locus_tag="OHO85_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCQ2672156.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVCLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSRERQTGMQMVQSVLERIGEAK"
     gene            20833..22053
                     /gene="wcaL"
                     /locus_tag="OHO85_002806"
     CDS             20833..22053
                     /gene="wcaL"
                     /locus_tag="OHO85_002806"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCQ2672157.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPLHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAILDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELVSLLQAL"
     gene            22064..23458
                     /gene="wcaM"
                     /locus_tag="OHO85_002807"
     CDS             22064..23458
                     /gene="wcaM"
                     /locus_tag="OHO85_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCQ2672158.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPAGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHIIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVSNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLALHNKPQHLFMRNINV
                     MQESTVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            23633..24550
                     /gene="galF"
                     /locus_tag="OHO85_002808"
     CDS             23633..24550
                     /gene="galF"
                     /locus_tag="OHO85_002808"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCQ2672159.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETARS
                     QVLAKRMPGDLSEYSLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVG
                     RYVLSADIWPELERTQPGAWGRIQLTDAIAELAKKQFVDAMLMTGDSYDCGKKMGYMQ
                     AFVKYGLRNQKEGVKFRKGIEELLSKCIV"
BASE COUNT         6010 a         6177 c         6706 g         5707 t
ORIGIN      
        1 acctaaagaa actcctaaaa accatattga atgacactta atataattct taaaaatagc
       61 caattgccga attgttacct tgcctgctat tccgttagct gtaacacttc ctcctgcatt
      121 attggagagc caatattcaa ttaccataat gtcaccttat attcggaagt taatttccgc
      181 aataaccctt tctcatcaac gattgtatgc ggagagggtt tgaaaaatta agctaaatca
      241 gtgtattggt agctaaaaag ccaggggcgg tagcgtgtct ggatgcctga aagaccagtc
      301 tgaattattc tgtcaatagc ctgcggatga aatcaatttt tttaggactg atgccagtta
      361 aatttttatt caattaactg caaggtaaat gtgcatggcg acattgctat taatagtgca
      421 caggataatt actctgccaa agtgataaat aaacaatgat gaaatccaaa atgaaattga
      481 tgccattatt ggtgtcagta accttgataa gcggttgcac agtacttccg ggcagcaata
      541 tgtcgacgat gggcaaagac gtcatcaaac agcaggacgc tgatttcgat ctcgacaaaa
      601 tggtgaatgt ttatccgctg accccgcgcc tgattgacca attacgccca cgcccgaatg
      661 tagcgcgccc caatatgacg ctggaaagcg agatcgcgaa ttaccagtat cgcgtcgggc
      721 cgggggacgt tctgaatgtc accgtctggg atcacccgga actcaccacg ccagccggtc
      781 agtaccgcag ctccagcgac accggcaact gggtacagcc tgacggcact atgttttacc
      841 cgtatatcgg caaggttcac gtagtcggga aaacgctcgc tgaaatccgc agtgatatta
      901 ccgggcgctt agcgacgtac atcgctgacc cgcaggtgga cgttaatatc gccgccttcc
      961 gctcacaaaa ggcctatatc tcaggtcagg tgaataaatc cggtcaacag gcgatcacca
     1021 acgtaccact gaccattctc gatgccatca acgccgcagg tggcctgacc gacaccgctg
     1081 actggcgcaa cgtggtgcta acacacaatg gtcgtgaaga acgcatttct ttgcaggcgc
     1141 tgatgcaaaa cggcgacctc aatcagaacc gactgcttta ccccggcgat attctctacg
     1201 tgccgcgtaa tgatgatctg aaagtgtttg tgatgggtga agtgaagaaa cagagcaccc
     1261 tgaaaatgga ctttagcggc atgaccctga ctgaagctct gggcaatgcc gaaggtatcg
     1321 acatgaccac ctccaacgcc agcggcatct ttgtcattcg tccgttgaaa ggcgagggcg
     1381 ggcgtaacgg caagattgcc aatatctacc agctggatat gtccgatgcg acgtcgctgg
     1441 tgatggcgac agaattccgc ctgcaacctt atgacgtggt gtatgtcacc accgccccgg
     1501 tttcccgctg gaaccgtctg atcaatcagt tgctgccaac tattagcggt gtccgttaca
     1561 tgacggatac agccagcgac attcataact ggtaatcgtc atgtttaaca acatattagt
     1621 tgtctgtgtc ggcaatattt gccgttcccc gacggcggaa cgcttactgc aacgttatca
     1681 cccggagctg aaagtggagt ccgctggact cggcgcgctg gtcggtaagg gcgctgatcc
     1741 taccgctatc agcgtcgccg cagaacatca actgtctctg gaaggtcact gtgcacggca
     1801 aatcagccgc cgcctgtgtc gcaactatga cctgattttg accatggaaa agcgccatat
     1861 cgaacgctta tgcgagatgg cacctgagat gcgcggcaaa gtgatgctgt ttggtcactg
     1921 ggataacgaa tgtgaaatcc ccgatccgta tcgcaaaagc cgggaaacgt ttgcagcggt
     1981 gtacacatta cttgaacggt ctgcccgcca gtgggcgcag gcattgaacg cagagcaggt
     2041 ataagaatga cagaaaaagt aaaacaacat gccgctccgg taacgggcag tgatgaaatc
     2101 gatattggtc gcctggtcgg caccgtcatt gaagcgcgct ggtgggtgat tggcatcacc
     2161 gctgtatttg ccctctgtgc tgtggtttac actttcttcg ccacgccgat ttatagtgcc
     2221 gacgcactgg tacaaatcga acaaaacagc ggcaattcgt tagtgcagga cattggctcg
     2281 gcattagcca acaaaccgcc tgcatcggac gccgagatcc agttgattcg ttcgcgtctg
     2341 gtgcttggta aaacggtgga cgatctagac ctcgatattg cggtgagcaa aaacacgttc
     2401 ccgattttcg gtgcgggttg ggatcgcctg atgggacgtc agaacgagac ggtgaaagtg
     2461 accaccttta accgcccgaa agagatggcg gaccaggtgt ttacgcttaa tgtgctggat
     2521 gacaaaaact acactctgag cagtgatggc ggctttagcg cccgtgggca agcgggccag
     2581 atgctgaaaa aagaaggcgt cacgctgatg gttgaagcca ttcacgcccg cccgggcagt
     2641 gagtttaccg tcaccaaata ctccacgttg gggatgatca accaactgca aaacagcctg
     2701 acggtaacgg agaacggcaa agacgcaggc gtactgagcc tgacttatac cggtgaagat
     2761 cgcgaacaga tccgcgacat tcttaacagc atcgcccgta actatcagga acaaaatatt
     2821 gagcgcaaat cggcggaagc gtcgaaaagc ctcgctttcc tcgcgcaaca gttaccggaa
     2881 gtacgtagcc gcctcgatgt tgccgaaaac aaactgaatg ccttccgtca ggataaagat
     2941 tctgttgatc tgccgctgga agcgaaagcg gtgctcgatt cgatggtgaa catcgacgcg
     3001 cagttgaacg aactgacctt taaagaggcg gaaatctcca agctgtacac caaagttcac
     3061 ccggcgtacc gcacgctgct ggagaaacgt caggcgctgg aagaagagaa agccaaactt
     3121 aacggtcgcg taacggcgat gccgaaaacc cagcaggaga ttgtgcgcct gacccgcgat
     3181 gtcgagtctg gtcagcaggt ctatatgcaa ctgctgaata aagagcagga gctgaaaatc
     3241 accgaagcca gcaccgtcgg cgatgtgcgc attgttgacc cggcaatcac ccagcctggc
     3301 gtgctgaaac cgaagaaagg actgattatc ctcggcgcaa ttatcctcgg cctgatgctc
     3361 tctatcgtgg gtgtgctgct gcgctcgttg tttaatcgcg gcatcgaaag cccgcaggtg
     3421 ctggaagaac atggtatcag cgtctatgcc agcatcccgt tgtcggaatg gcagaaagcg
     3481 cgcgatagcg tcaaaaccat caaagggatt aaacgctata aacagagcca gctactggcg
     3541 gtggggaatc caaccgatct ggcgattgaa gccatccgca gtctgcgtac cagtctgcac
     3601 ttcgcgatga tgcaggcgca gaacaatgtg ttgatgatga caggggtgag cccgtcaatc
     3661 ggtaaaacct ttgtctgcgc caacctggcg gcggtgatca gtcagaccaa taaacgggtg
     3721 ttgttgatcg actgcgatat gcgcaaaggc tacacccacg agctgttggg taccaataac
     3781 gtcaatggcc tgtcggaaat tctgattggc cagggcgata ttactaccgc agctaaaccg
     3841 acctctattg ccaaatttga cctgatcccg cgcggtcagg taccgccaaa tccttctgag
     3901 ctgttgatga gcgaacgctt tgccgaactg gtgaactggg cgagtaaaaa ctatgacctg
     3961 gtgttgattg atacgccgcc gattctggca gtgaccgatg cggcaattgt tggtcgtcat
     4021 gttggaacca cgttaatggt ggcgcgttat gcggtcaaca cattgaaaga agtggaaacc
     4081 agtctgagcc gctttgagca aaatggtatt ccggtgaaag gggtgattct gaactccatc
     4141 ttccgccgcg ccagcgcgta tcaggattat ggctattacg aatacgaata taagtcggat
     4201 gcgaaataac gacatccgct gtcggatgcg atgctgacgc atcttatccg acctaccttc
     4261 acgccgcatc cggcaagcaa accagctccg taggccggat aaggcgctca cgccgcatcc
     4321 ggcaacagaa cccgtaaaca agcttataaa ccggcagaac atctcgacac tccgggagaa
     4381 caacctatga aagacaatcc gctgatctca atctatatgc cgacctggaa ccgccaacaa
     4441 ctggcgattc gggcgataaa atcggtcctg cgccaggact acagcaactg ggagatgatc
     4501 atcgtggatg attgctccac ctcctgggaa caactgcaac agtacgtcac cgccctcaac
     4561 gatccgcgta ttacctacat tcacaacgac attaacagcg gggcgtgcgc ggtacgtaac
     4621 caggcgatta tgctggcgca aggggaatac attaccggga tcgatgacga tgatgaatgg
     4681 acgcccaacc gcctgagcgt cttcctcgcc cataaacagc aactggtcac tcacgccttt
     4741 ttgtacgcta acgactacgt atgccaggga gaagtttatt cccaaccggc aagcttacca
     4801 ctgtatccca aatcgccgta ctcacgccgc ctgttctata aacgcaatat cattggcaat
     4861 caggttttta cctgggcgtg gcgtttcaaa gagtgcttgt tcgataccga actgaaagca
     4921 gcacaggatt acgacatctt cctgcggatg gtggtggagt atggcgagcc gtggaaagtg
     4981 gaagaggcga cgcagatcct gcatatcaat cacggtgaaa tgcagatcac ctcgtcaccg
     5041 aaaaaattct ccgggtactt ccacttttac cgcaagcaca aagataaatt tgaccgtgca
     5101 agcaaaaaat atcagctgtt taccctctac cagatccgca ataaacgtat gacctggcgc
     5161 actttgctga cgctgctttc ggtgcgtaac ggcaaacgtc tggctgatgg tattcggggg
     5221 cgctaaagat gctggaagat ctgcgcgcca acagctggag tttacgtccg tgctgcatgg
     5281 ttctagccta tcgcgtcgct catttttgtt cggtgtggcg caaaaagaac gtcctcaaca
     5341 atatttgggc ggccccgctg ctggtgctgt atcgcattat caccgaatgc tttttcggtt
     5401 atgaaatcca ggctgccgcg accattggcc gccgctttac tatccatcac ggttacgccg
     5461 tagtgatcaa taaaaacgtg gtggcggggg atgatttcac cattcgccac ggcgtcacta
     5521 tcggcaatcg tggtgccgat aacatggcat gtccacatat tggcaacggc gtcgaactcg
     5581 gtgccaacgt tattattctt ggtgatatca cgcttggtaa caacgtcacc gtgggtgcgg
     5641 gcagcgtggt gctcgactct gtcccggaca acgcgctggt ggtgggagaa aaagcgcgag
     5701 tgaaggtaat taaatgaata ttttgcaatt taatgtgcga ctggcggaag gcggggcagc
     5761 aggtgtggcg ttagatctcc accagcgtgc gctgcaacag gggctagcgt cacattttgt
     5821 ctacggttac ggcaaaggcg gcaaagagag cgtcagccat caaaactatc cgcaggtcat
     5881 caaacatacg ccgcggatga ctgcaatggc gaatattgtc ctgtttcgtc tgtttaatcg
     5941 cgatctgttt ggcaatttca atgagttata tcgcaccatt actcgcacac cgggtccggt
     6001 ggtcctgcat tttcatgtgc tgcacagcta ctggctaaat cttaagagcg tggtgcgctt
     6061 ttgcgaaaaa gtgaaaaacc ataaaccgga cgtcactctg gtctggacgc tgcacgacca
     6121 ctggagtgtt accggacgct gcgcctttac cgacggttgc gaaggctgga aaacaggctg
     6181 ccagaaatgc ccgaccttaa ataattatcc gccggtgaag attgatcggg cgcaccagct
     6241 ggtggcgggc aaacgccagt tattccgtga gatgctggcg ctgggctgtc agtttatttc
     6301 ccccagccag catgtggctg acgctttcaa tagcctgtac ggtccagggc gttgccggat
     6361 tatcaataac ggtattgata tggcaaccga agcgattctg gcggatctgc ctccggtgcg
     6421 cgaaacccag ggtaagccga aaatcgcggt ggtggcgcat gacctgcgtt acgacggcaa
     6481 gactaaccag caactggtgc gtgagatgat ggcgctgggc gacaaaatcg aactgcatac
     6541 ctttggtaag ttctcgccgt tcaccgctgg taacgtggtt aatcacggct ttgaaaccga
     6601 caaacgtaag ctgatgagcg cgctcaatca gatggatgcg ctggtattca gttctcgcgt
     6661 cgataactac ccgctgattt tgtgtgaggc gctatcgatt ggcgtgccgg tgatcgccac
     6721 ccatagcgat gcggcgcggg aagtgctgca aaaatccggt ggtaaaaccg tcagtgaaga
     6781 agaggtgctg caactggtgc agttaagcaa accggaaatc gcgcaggcga tatttggtac
     6841 cacgctggct gagttcagcc aacgcagccg cgccgcctac agtggacaac agatgctgga
     6901 ggagtatgtc aacttctatc agaatctgta gctacctgct gctgccgctg atctacctgc
     6961 tggttaacgt caaaatcgcc cagcttggtg aaagtttccc catcactatc gtcacttttt
     7021 tacctgtctt gttactgctg tttttagaac gcatcagcgt taaaaaattg atgattgcct
     7081 tagggattgg tgcgggactc acggcgttca actatctgtt tggtcagtcg ctggatgcca
     7141 gtaaatacgt cacttcaacc atgctgtttg tctatattgt gatcattatt ggcatggtgt
     7201 ggagtattcg ctttaaaacg atttcgccac acaaccatcg caagatatta cgtttctttt
     7261 atctggtggt cgggttggtg gtggcgctgg cggcggtgga gatggcgcaa attatcctca
     7321 ccggcggcag cagtattatg gagtcgattt cgaaatatct gatttacagc aacagctatg
     7381 tactgaattt cattaaattc ggcggcaagc gcacgacagc actttatttc gaaccggcat
     7441 ttttcgctct ggcattaatc tcaatttggc tcagcatcaa acagtttggt atcaaaacgc
     7501 ctaaaaccga tgctatgatt ctcgcaggga taatattatc cggatcgttt tcaggggtta
     7561 tgacctttat cctgttttat ttgctggagt gggcatttca atatctgaat aaagaggcga
     7621 ttaagaaaaa gttaccgtta gcattgattt ctctggctgt attcctggtt ggtgtggtaa
     7681 tcgcgtttcc ttatatttcc acccgtctgg gcgatttagg tacggaagga tcgtcatcgt
     7741 attatcgtat tgtcggtccg ctggtgatgg tcggttattc tttgacccat attgacggtg
     7801 tagtcagatt tggctcactt tatgaatatg tcgcatcatt cggaatattt aacggtgcgg
     7861 atgtcggaaa aaccatagac aatggtttgt atctgctgat tatttatttt tcctggttcg
     7921 cggtgttttt atcgctgtgg tacatgggga aagtgataaa aatgatgatc aacgcttttg
     7981 gtgataaccg caattttcgc gtgcaattat atctttttac tccggtatcg ctgtttttta
     8041 ccggttcgat atttagcccg gaatatgcat ttttaatcgt ctgtccgttt attttgcgaa
     8101 aagcgttaaa tattacgagg taagaataag aacatgttgc ttagcataat cactgtcgcg
     8161 tttcgtaacc tcgaagggat agtcaaaaca catgcctcgc tggcgcatct ggcgcaggcg
     8221 gacgatatca gcttcgaatg gattgttgtc gatggcggtt ccaacgacgg tacccgtgag
     8281 tatctggaaa atctcaatag tatctataac ctacgctttg tcagcgagcc agataacggt
     8341 atctacgacg ccatgaacaa gggcattgcg atggcacaag gcaagttcgc gttgtttctc
     8401 aattcgggcg atatttttca tcaggatgcc gcaaattttg tccgtaagtt aaaaatgcaa
     8461 aaagataaca tgatgatcac cggcgatgcg ctgctggatt ttggcgacgg gcataaaatt
     8521 aagcgtagtg ctaaaccggg ttggtatatt tatcacagcc tgcccgccag ccatcaggcg
     8581 atatttttcc cggtatccgg tttgaaaaaa tggcgttatg acctggaata taaagtttct
     8641 tctgattacg cgctggcagc taaaatgtat aaagcaggtt atgcatttaa aaaactcaat
     8701 ggcctggtgt ctgaattttc catgggtggg gtatcaacca ccaataatat ggaattgtgt
     8761 gctgacgcca aaaaagtcca acggcagata ttacatgtgc ctggcttctg ggcagaatta
     8821 tcctggcatt tacgccaacg tactacctca aagacgaaag ccttatataa caaaagctga
     8881 atataaggga aaacgatgca ggatttaagc ggtttctcgg tgccgaaagg gttccggggc
     8941 ggcaacgcta ttaaagtgca attatggtgg gcagtacagg caacaatatt tgcctggtcg
     9001 ccacaagtat tgtatcgctg gcgggctttt ttattacgtt tattcggagc aaaaatagga
     9061 aaaaacgtag ttattcgtcc gtcagtaaaa attacctatc cgtggaaatt aaccttaggt
     9121 gattacgcgt gggtcggcga tgacgtcaat ttatataccc tcggtgaaat aaccattggc
     9181 gcacattcgg tgatatcgca aaaaagttat ttatgcaccg gtagccacga ccatgcaagt
     9241 caacatttca ccattaacgc cacgcctatt gtgattggcg agaaatgctg gctggcaacc
     9301 gatgtctttg ttgccccagg cgtcacaatc ggcgacggca ccgtcgtggg tgcacgaagc
     9361 agtgttttta aatcgcttcc ggcaaatgtg gtttgccggg ggaatcccgc agtggtgata
     9421 cgcgaacgcg ttgaaactga ataaattcaa aaatacagag gaataagaca tgtcaaaagt
     9481 cgctctcatc accggtgtaa ccggacaaga cggttcttac ctggcagagt ttctgctgga
     9541 aaaaggttac gaggtgcatg gtattaagcg tcgcgcatcg tcattcaaca ccgagcgcgt
     9601 ggatcacatt tatcaggatc cgcacacctg caacccgaaa ttccatctgc attatggcga
     9661 cctgagtgat acctctaacc tgacgcgcat tttgcgtgaa gtacagccgg atgaagtgta
     9721 caacctgggc gcaatgagcc acgttgcggt ctcttttgag tcaccagaat ataccgctga
     9781 cgtcgacgcg atgggtacgc tgcgcctgct ggaggcgatc cgcttcctcg gtctggaaaa
     9841 gaaaactcgt ttctatcagg cttccacctc tgaactgtac ggtctggtgc aggaaattcc
     9901 gcagaaagaa accacgccgt tctacccgcg atctccgtat gcggtcgcca aactgtatgc
     9961 ctactggatc accgttaact accgcgaatc ctacggcatg tacgcctgta acggtattct
    10021 cttcaaccat gaatccccgc gccgcggcga aaccttcgtt acccgcaaaa tcacccgcgc
    10081 aatcgccaat atcgcccagg ggctggagtc gtgcctgtac ctcggcaata tggattccct
    10141 gcgtgactgg ggccatgcca aagactacgt aaaaatgcag tggatgatgc tgcaacagga
    10201 acaaccggaa gatttcgtta tcgcgaccgg cgttcagtac tccgtacgtc agttcgtgga
    10261 aatggcggca gcacagctgg gcatcaaact gcgctttgaa ggcacgggcg ttgaagagaa
    10321 gggcattgtg gtttccgtca ccgggcatga cgcgccgggc gttaaaccgg gtgatgtgat
    10381 tatcgctgtt gacccgcgtt acttccgtcc ggctgaagtt gaaacgctgc tcggcgaccc
    10441 gaccaaagcg cacgaaaaac tgggctggaa accggaaatc accctcagag agatggtgtc
    10501 tgaaatggtg gctaatgacc tcgaagcggc gaaaaaacac tctctgctga aatctcacgg
    10561 ctacgacgtg gcgatcgcgc tggagtcata agcatgagta aacaacgagt ttttattgct
    10621 ggtcatcgcg ggatggtcgg ttccgccatc aggcggcagc tcgaacagcg cggtgatgtg
    10681 gagctggtat tacgcacccg cgatgaactg aacctgctgg acagccgcgc ggtgcatgat
    10741 ttctttgcca gcgaaagcat tgacctggtc tatctggcgg cggcgaaagt gggcggcatt
    10801 gttgccaaca acacatatcc ggcggatttc atctaccaga acatgatgat tgagagcaac
    10861 atcattcacg ccgcgcatca gaacgacgtg aacaaactgc tgtttctcgg atcgtcctgt
    10921 atctacccga aactggcaaa acagccgatg gcagaaagcg agttattgca gggcacgctg
    10981 gagccgacca acgagcctta tgccattgcc aaaattgccg ggatcaaact gtgcgaatct
    11041 tacaaccgcc agtacggacg cgattaccgc tcagtcatgc cgaccaacct gtatggcccg
    11101 catgacaact tccacccgag taattcgcat gtgatcccag cattgttgcg tcgcttccac
    11161 gaggcgacgg cacagaatgc gccggacgtt gtggtatggg gcagcggtac accgatgcgt
    11221 gaattcctgc acgtcgatga tatggcggcg gccagcattc acgtgatgga gctggcgcac
    11281 gaagtctggc tggagaacac ccagccgatg ctgtcgcaca ttaacgtcgg taccggtgtt
    11341 gactgcacta tccgcgagct ggcgcaaacc atcgccaaag tggtggatta caaaggtcgg
    11401 gtggtttttg atgccagcaa accggatggc acgccgcgca aactgctgga tgtgacgcgc
    11461 ctgcatcagc ttggctggta tcacgaaatc tcactggaag cggggcttgc cagcacttac
    11521 cagtggttcc ttgagaatcg agaccgcttt cgggggtaat gatgttttta cgtcaggaag
    11581 actttgccac ggtagtgcgc tccacaccgc ttgtctctct cgactttatt gccgagaaca
    11641 gtcgcggcga gtttctgctt ggcaaaagaa ccaaccgccc ggcgcagggt tactggtttg
    11701 tgccgggagg gcgcgtgcag aaagacgaaa cgctggaagc cgcatttgag cggctgacga
    11761 tggcggaact ggggctgcgt ctgccgataa cagcaggcca gttttacggt gtctggcagc
    11821 acttttatga cgataacttc tctggcacgg atttcactac tcactatgtg gtgctcggtt
    11881 ttcgcttcag agtagcggaa gaagaactgt tactgccgga tgagcagcat gacgattacc
    11941 gctggctgac gccggacgca ctgctcgcca gtgacaatgt tcacgctaac agccgcgcct
    12001 attttctcgc tgagaagcgt gccggagtac ccggattatg aaaatactgg tctacggcat
    12061 taactactcg ccggagttaa ccggcatcgg caaatacacc ggcgagatgg tggaatggct
    12121 ggcggcacaa ggtcatgagg tgcgggtcat taccgcaccg ccttactacc cgcaatggca
    12181 ggtgggtgag aactattccg cctggcgcta caaacgagaa gagggggccg ccacggtgtg
    12241 gcgctgcccg ctgtatgtgc caaaacagcc gagcaccctg aaacgcctgt tgcatctcgg
    12301 cagttttgcc gtcagcagtt tctttccgct gatggcgcaa cgtcgctgga agccggatcg
    12361 cattattggc gtggtaccaa cgctgttttg cacgccggga atgcgcctgc tggcgaaact
    12421 ctctggcgcg cgtaccgtgc tgcatattca ggattacgaa gtggacgcca tgctggggct
    12481 gggccttgcc ggaaaaggca aaggcggcaa agtggcacag ctggcaacgg cgttcgagcg
    12541 tagcggactg cataacgtcg ataacgtctc cacgatttcg cgttcgatga tgaataaagc
    12601 catcgaaaaa ggcgtggcgg cggaaaacgt catcttcttc cccaactggt cggaaatcgc
    12661 ccgttttcag catgttgcag atgccgatgt tgatgccctt cgtaaccagc ttggcctgcc
    12721 ggataacaaa aaaatcattc tttactccgg caatattggt gaaaagcagg ggctggaaaa
    12781 cgttattgaa gctgccgatc gtctgcgcga tgaaccgctg atttttgcca ttgtcgggca
    12841 gggcggcggc aaagcgcggc tggaaaaaat ggcgcagcag cgtggactgc gcaacatgca
    12901 atttttcccg ctgcaatcgt atgacgcttt acccgcactg ctgaagatgg gcgattgcca
    12961 tctggtggtg caaaaacgcg gcgcggcaga tgccgtattg ccgtcgaaac tgaccaatat
    13021 tctggcagta ggcggtaacg cagtgattac cgcagaagcc cacacagaac tggggcagct
    13081 ttgcgaaatc tttccgggca ttgcggtttg cgttgaaccg gaatcggtcg aggcgctggt
    13141 ggcggggatt cgtcaggcgc tcctgctgcc caaacacaac acggtggcac gtgaatatgc
    13201 cgaacgcacg ctcgataaag agaacgtgtt acgtcaattt ataaatgata ttcggggata
    13261 attatggcgc agtcgaaact ctatccagtt gtgatggcag gtggctccgg tagccgctta
    13321 tggccgcttt cccgcgtact ttaccccaag cagtttttat gcctgaaagg cgatctcacc
    13381 atgctgcaaa ccaccatctg ccgcctgaac ggcgtggagt gcgaaagccc ggtggtgatt
    13441 tgcaatgagc agcaccgctt tattgtcgcg gaacagctgc gtcaactgaa caaactcacc
    13501 gagaacatta ttctcgaacc tgcagggcgt aacactgcac ctgccattgc gctggcggcg
    13561 ctggcggcaa aacgtcatag cccggagatc gacccgttaa tgctggtatt ggcggcggat
    13621 catgtgattg ccgatgaaga cgcgttccgt gccgccgtgc gtaatgccat gccgtatgcc
    13681 gaagcgggca agctggtgac cttcggcatt gtgccggatc tacctgaaac cggatatggc
    13741 tatattcgtc gcggtgaagt atctgcgggt gagcaggata cggtggcctt tgaaatggcg
    13801 cagtttgtcg aaaaaccgaa tctggaaacc gcccaggcct atgtggcaag cggcgaatat
    13861 tactggaaca gcggcatgtt cctgttccgc gccggacgct atctcgaaga actgaaaaaa
    13921 tatcgcccgg atattctcga cgcctgtgaa caagcgatga gcgccgtcga tccggatctc
    13981 gattttattc gcgtggatga agaagcgttt ctggcctgcc cggaagagtc ggtggattac
    14041 gcggtcatgg aacgtacggc agatgccgtt gtggtgccga tggatgcggg ctggagcgat
    14101 gtcggttcct ggtcttcatt atgggagatc agcgcccaca ccgccgaggg caacgtttgc
    14161 cacggtgatg tgattaatca caaaactgaa aacagctatg tgtacgccga atctggcctg
    14221 gtcaccaccg tcggggtgaa agatctggta gtggtgcaga ccaaagatgc ggtgctgatt
    14281 gccgaccgta acgcggtgca ggatgtgaaa aaagtggtcg agcagatcaa agccgatggt
    14341 cgccatgagc atcgggtgca tcgcgaagtg tatcgtccgt ggggcaaata tgactctatc
    14401 gacgcgggcg accgctacca ggtgaaacgc atcaccgtga aaccgggcga gggcttgtcg
    14461 gtacagatgc accatcaccg cgcggaacac tgggtagtgg tcgcgggaac ggcaaaagtc
    14521 accattgatg gtgatatcaa actgcttggt gaaaacgagt ccatttatat tccgctgggg
    14581 gcgacgcact gcctggaaaa cccggggaaa attccgctcg atttaattga agtgcgctcc
    14641 ggctcttatc tcgaagagga tgatgtggtg cgtttcgcgg atcgctacgg acgggtgtaa
    14701 acgtcgcatc aggcgctgtt tgtcggatgc ggcgtgaacg ccttatccga cctacggttc
    14761 ggatttgtag gcctgataag acgcggcagc gtcgcatcag gcgctggctg tcggatgcgg
    14821 cgtgaacgcc ttatccgacc tacggttcgg ttttgtaggc ctgataagac gcggcagcgt
    14881 cgcatcaggc aatgaatgcg aaaccgcggt gtaaataacg acaaaaacaa aattggccgc
    14941 ttcggtcagg gccaactatt gcctgaaaaa gggtaacgat atgaaaaaat taacctgctt
    15001 taaagcctat gatattcgcg ggaaattagg cgaagaactg aatgaagata ttgcctggcg
    15061 cattggtcgc gcttatggcg aatttctcaa accgaaaacc attgtgttag gcggtgatgt
    15121 ccgcctcacc agcgaaacct taaaactggc gctggcgaga ggtttacagg atgcgggcgt
    15181 cgatgtgctg gatatcggca tgtccggcac cgaagagatc tatttcgcca cgttccatct
    15241 cggcgtggat ggcggcattg aagttaccgc cagccataat ccgatggatt ataacggcat
    15301 gaagctggtg cgcgaggggg ctcgcccgat cagcggtgat accggactgc gcgacgtcca
    15361 gcgtctggct gaagccaacg actttcctcc cgtcgatgaa accaaacgcg gtcgctatca
    15421 gcaaattacc ctgcgtgatg cttacgttga tcacctgttc ggttatatca atgtcaaaaa
    15481 cctcacgccg ctcaagctgg tgatcaactc cgggaacggc gcagcgggtc cggtggtgga
    15541 cgccattgaa gcccgcttta aagccctcgg cgcaccagtg gaattaatca aagtgcacaa
    15601 cacgccggac ggcaatttcc ccaacggtat tcctaacccg ctactgccgg aatgccgcga
    15661 cgacacccgc aatgcggtca tcaaacacgg cgcggatatg ggcattgcct ttgatggcga
    15721 ttttgaccgc tgcttcctgt ttgacgaaaa agggcagttt atcgagggct actacattgt
    15781 cggcctgctg gcagaagcgt tcctcgaaaa aaatcccggc gcgaagatca tccacgatcc
    15841 acgtctctcc tggaacaccg ttgatgtggt gactgccgca ggtggcacgc cggtaatgtc
    15901 gaaaaccgga cacgccttta ttaaagaacg tatgcgcaag gaggacgcca tctacggtgg
    15961 cgaaatgagc gcccaccatt acttccgtga tttcgcttac tgcgacagcg gcatgatccc
    16021 gtggctgctg gtcgccgaac tggtgtgcct gaaagagaaa acgctgggcg aactggtacg
    16081 cgaccggatg gcggcgtttc cggcaagcgg tgagatcaac agcaaactgg cgcaacccgt
    16141 tgaggcgatt aaccgcgtgg aacagcattt tagccgtgag gcgctggcgg tggatcgcac
    16201 cgatggcatc agcatgacct ttgccgactg gcgctttaac ctgcgctcct ccaacaccga
    16261 accggtggtg cggttgaatg tggaatcgcg cggtgatgtg ccgctgatgg aagcgcgaac
    16321 gcgaactctg ctggcgttgc tgaacgagta atgtcggatc ttcccttacc ccattgcggg
    16381 taaggggcta ataacaggaa caacgatgac aaatctaaaa aagcgcgagc gagcgaaaac
    16441 caatgcatcg ttaatctcta tggtgcaacg cttttcagat atcaccatca tgtttgccgg
    16501 actatggctg gtttgcgaag tgagcggact gccattcctc tacatgcacc tgttggtggc
    16561 gctgattacg ctggtggtgt tccagatgct gggcggcatc accgattttt atcgctcatg
    16621 gcgcggtgtt cgggcagcga cagaatttgc cctgttgcta caaaactgga ccttaagcgt
    16681 gattttcagc gccggactgg tggcgttcaa caatgatttc gacacgcaac tgaaaatctg
    16741 gctggcgtgg tatgggctga ccagtatcgg actggtggtt tgccgttcat gtattcgcat
    16801 tggggcgggc tggctgcgta atcatggcta taacaagcgt atggtcgccg tagcgggaga
    16861 tttagccgcc ggacaaatgc tgatggagag cttccgtaat cagccgtggt tagggtttga
    16921 agtggtgggg gtttaccacg acccgaaacc gggcggcgtt tctaacgact gggcgggcaa
    16981 tctgcaacag ctggtcgagg atgcgaaagc gggcaaaatt cataacgtct atatcgcgat
    17041 gcagatgtgc gacggcgcgc gagtgaaaaa actggtccat caactggcgg ataccacctg
    17101 ttcggtgctg ctgatccccg atgtctttac cttcaacatt ctccattcac gtctcgaaga
    17161 gatgaacggc gttccggtgg tgccgcttta cgacacgccg ctttccgggg ttaaccgcct
    17221 gctcaaacgt gcggaagaca ttgtgctggc gacgcttatt ctgttgctga tctccccggt
    17281 gctgtgctgt attgcgctgg cggtgaaact cagttcacca gggccggtta ttttccgcca
    17341 gactcgctat ggcatggatg gcaagccgat caaagtgtgg aagtttcgtt ccatgaaagt
    17401 gatggagaac gacaaagtgg tgacccaggc gacgcagaac gatccgcgcg tcaccaaagt
    17461 ggggaacttt ctgcgccgca cctcgctgga tgaattgccg cagtttatca atgtgctgac
    17521 cggggggatg tcgattgtcg gtccacgtcc acacgcggtg gcgcataacg aacagtatcg
    17581 acagctcatt gaaggctaca tgctgcgcca taaggtgaaa ccgggcatta ccggctgggc
    17641 gcagattaat ggctggcgcg gcgaaaccga cacgctggag aaaatggaaa aacgcgtcga
    17701 gttcgacctt gagtacatcc gcgaatggag cgtctggttc gatatcaaaa tcgttttcct
    17761 gacggtattc aagggcttcg ttaacaaagc ggcatattga tatgagctta cgtgaaaaga
    17821 ccatcagcgg cgcgaagtgg tcggcgattg ccacggtgat catcatcggc ctcgggctgg
    17881 tgcagatgac cgtgctggcg cggattatcg acaaccacca gttcggtttg cttaccgtgt
    17941 cgctggtgat catcgcgctg gcagatacgc tttctgactt cggtatcgct aactcgatta
    18001 ttcagcgaaa agaaatcagc caccttgaac tcaccacgct gtactggctg aacgtcgggc
    18061 tggggcttgt ggtgtgcgtg gcggtgtttt tgttgagtga tgccattggc gacgtgctga
    18121 ataacccgga cctggcaccg ttgattaaaa cattgtcgct ggcgtttgtg gtgatccccc
    18181 acgggcaaca gttccgcgcg ttgatgcaga aggagctgga gttcaacaaa atcggcatga
    18241 tcgaaaccag cgcggtactg gcgggcttca cttttacggt ggttagcgcc catttctggc
    18301 cgctggcgat gaccgcgatc ctcggttatc tggtcaatag tgcggtgaga acgctgctgt
    18361 ttggctactt tggacgcaaa atttatcgcc ccggtctgca tttctcgctg gcgtcggtgg
    18421 caccgaactt acgctttggt gcctggctga cggcggacag catcatcaac tatctcatca
    18481 ccaacctttc aacgctggtg ctggcgcgta ttctcggcgc gggcgtagca gggggataca
    18541 acctggcgta caacgtggcc gttgtgccgc cgatgaagct gaacccaatc atcacccgcg
    18601 tgttgtttcc ggcgtttgcc aaaattcagg acgacaccga aaagctgcgt attaacttct
    18661 acaaactgct gtcggtggtg gggattatca actttccggc gctgctgggg ctgatggtgg
    18721 tgtcgaataa cttcgtaccg ctggtattcg gcgaaaagtg gaacagcatt attccggtgc
    18781 tgcaattgct gtgtgtggtg gggctactgc gctccgtggg taacccgatt ggttcgctgc
    18841 tgatggcgaa agcgcgggtc gacatcagct ttaaattcaa cgtattcaaa acctttctgt
    18901 ttattccggc gattgttatt ggtgggcaga tggcgggcgc aatcggcgtc acgctcggtt
    18961 tcctgctggt gcagattatc aacaccattc tgagttactt cgtgatgatt aaaccggttc
    19021 ttggttccag ttatcgccag tacatcctga gtttgtggct gccgttttac ctctcgctgc
    19081 cgacgctggt ggttagttat gcgctgggca ttgtgctgaa agggcaactg gcgctgggga
    19141 tgctgctggc ggtgcaaata gccgcgggcg tgctggcgtt tgtggtgatg attgtgctgt
    19201 cgcgccatcc gctggtggtg gaagtgaagc gtcagttttg tcgcagcgaa aaaatgaaaa
    19261 tgcttttacg ggcggggtga gttgctgttt tcggttccga caacggttgc cggatgcggc
    19321 gtaaaacgcc ttatccatcc tacggttctg tgctcgggac ctgtaggcct gataagacgc
    19381 gtcagcgtcg catcaggcac tgaatgccgg atgcggcgtg aacgccttat ccggcctacg
    19441 gttctgtgct cgggtcttgt aggcctgata agacgcgtca gcgtcgcatc aggcaaaaag
    19501 cggcaacgaa caaaacaaac gcagcctcac ctggcatcaa atttatgagg aaaaaatgaa
    19561 attacttatt ctgggcaacc acacttgcgg caatcgtggc gacagcgcca tcctgcgcgg
    19621 cttacttgat gccatcaaca tcctcaatcc acaagccgaa gtggacgtga tgagccgcta
    19681 tccggtcagt tcttcctggc tgctcaaccg cccggtaatg ggcgatccgc tgttcctgca
    19741 aatgaaacaa cacaacagcg cggcgggcgt tgtcgggcgc gttaaaaaag tcctccgtcg
    19801 ccgctatcaa caccaggtat tgctctcacg cgtcactgac actggcaagc tgcgtaatat
    19861 cgccatcgcc cagggattca ccgacttcgt gtgcctgctg tcaggttacg acgccattat
    19921 tcaggtcggc ggatcgtttt ttgtcgatct ctacggcgtg ccgcagtttg aacatgcgct
    19981 ttgcacattt atggcgaaaa agccgctgtt tatgattggt cacagcgtcg gtcccttcca
    20041 ggatgagcaa tttaaccaac tggcgaacta cgtttttggt cactgtgacg cgctgatcct
    20101 gcgcgaatcg gtcagccttg atctgatgaa acgcagcaat atcaccactg caaaagtgga
    20161 acatggcgtc gatactgcgt ggctggtcga tcaccacacc gaagacttca ctgccagcta
    20221 tgccgtccaa cactggctgg acgttgccgc acaacagaaa acggtggcaa ttaccctgcg
    20281 cgaactggca ccgttcgaca aacgtctcgg caccactcaa caagcgtatg aaaaagcctt
    20341 tgccggggtg gtcaatcgca ttctcgacga agggtatcag gtgattgcgc tatccacctg
    20401 tacgggcatc gacagctata acaaagacga ccgcatggtg gcgctcaacc tacgccagca
    20461 catcagcgat cctgcccgtt atcacgtagt gatggatgaa ctcaacgatc tggaaatggg
    20521 caaaattctc ggagcctgtg aactcaccgt cggtacgcgc ctgcactccg ccattatctc
    20581 gatgaatttt gccactccgg caattgccat caactacgaa cataaatccg ccgggattat
    20641 gcaacaactg gggctgccag agatggcgat tgatatccgt catttactgg acggcagctt
    20701 gcaagcgatg gttgcggata ccttaggcca acttccggcg ctgaacgcgc gacttaacga
    20761 agccgtcagt cgcgaacgtc agacgggaat gcagatggtg caatctgtgc ttgagcgcat
    20821 cggggaggcg aaatgaaggt cggcttcttt ttactgaaat ttccgctgtc gtcggaaact
    20881 ttcgtcctca accaaattac cgcgtttatt gatatgggat ttgaggtgga gattgtcgcg
    20941 ctgcaaaaag gcgatacaga gaacacccac gcggcatgga cgaaatacaa ccttgccgcc
    21001 agaacccgct ggttacagga cgaaccaacg ggcaaagtgg cgaagctgcg ccaccgcgcc
    21061 agccagacct tacgcggcat tcatcgtaaa aatacctggc aggcgctcaa cctcaaacgc
    21121 tatggtgccg agtcgcggaa cctgattttg tctgccattt gcggtcaggt cgcaacaccg
    21181 ttacatgccg atgtctttat cgctcacttt ggtccggcag gggtaaccgc agcaaaactc
    21241 cgcgaactgg gtgtcattcg cggcaaaatt gccaccattt tccacggcat tgatatttcc
    21301 agtcgggaag tgctcaacca ctacactccc gaatatcaac aactgtttcg ccgtggcgac
    21361 ctgatgctac cgataagcga tctgtgggcc ggaaggctgc aaaaaatggg ctgcccgagg
    21421 gaaaaaatcg ccgtatcgcg catgggcgtg gacatgacgc gctttagccc gcgtccggtg
    21481 aaagcgcctg caacgccgct ggaaatcatt tccgtcgcac gcttaaccga gaaaaaaggc
    21541 ctgcatgtgg cgatcgaagc ctgccgtcag ttgaaagagc agggcgtggc atttcgctat
    21601 cgcatcctcg gcattggccc gtgggaacga cgcctgcgca ccctcatcga acaatatcaa
    21661 ctggaagatg tggtagagat gccgggcttt aaaccgagcc acgaagtgaa agcgatcctc
    21721 gacgacgcgg atgtcttcct cctgccatcg gtaacgggcg cggatggcga tatggaaggt
    21781 attccggtgg cgctgatgga ggcgatggcg gtcggcattc cggtggtttc tactctgcac
    21841 agcggaatac cggaactggt agaggccgat aaatccggct ggctggtgcc tgagaacgat
    21901 gctcgcgcac tggcgcaacg actggcggcg tttagccaac tggacaccga cgaactggct
    21961 ccggtcgtca aacgtgcgcg cgaaaaagtt gaacacgatt ttaaccagca ggtgatcaat
    22021 cgagaactcg tcagcttgct gcaagcttta tagaggagaa cgcatgccat ttaaaaaact
    22081 ctcccgacgc accttcctga cggcaagctc cgcgcttgcc ttccttcata cgcctttcgc
    22141 ccgagcactt cctgcccggc aaagcgttaa cattaacgac tacaacccac acgactggat
    22201 tgcttcattt aaacaagcct tcagcgaagg gcaaacggtc gtcgtgcctg ccggattcgt
    22261 ttgtgacaat atcaacaccg gcatcttcat tcctgcaggt aaaacgttac acatccttgg
    22321 aagcctgcgc ggtaacggca gagggcgatt tgtcttacag gacggcagcc aggtgacagg
    22381 ggaggagggc ggcagtatgc ataacatcac cctggatgtg cgcggctctg actgcaccat
    22441 caaagggctg gcgatgagcg gctttggtcc ggtaacgcag atttatatcg gcggcaaaaa
    22501 caaacgggtc atgcgcaacc tgaccatcga taacctcact gtcagccacg ctaattacgc
    22561 catcttacgc cagggatttc ataaccagat tatcggtgcc aacatcacca actgtaagtt
    22621 cagcgactta cagggcgacg ccattgaatg gaatgtggcg attaacgacc gcgatatttt
    22681 gatatccgac cacatcatcg agcgcatcaa ctgtaccaac ggcaaaatca actggggcat
    22741 cggcataggc cttgcgggaa gcacttacga taataattac ccggaagatc aggcagtgaa
    22801 aaactttgtc gtggcgaata tcacgggatc ggattgtcga cagttgatcc atgttgaaaa
    22861 tggtaaacat tttgttattc gtaatatcaa agcccgcaat atcacgccgg atttcagtaa
    22921 gaaagcaggc attgataacg ccacggtcgc tatttacggt tgtgacaatt tcgtgattga
    22981 taatattgaa atgattaata gcgccgggat gttaatcggc tatggggtaa ttaaaggcaa
    23041 atatctctcg ataccgcaaa atttccgagt gagtaatatt caactggata atacccacct
    23101 tgcttacaaa ttgcgcggca tccaaatctc cgccgggaat gccgtctcct ttgtggcact
    23161 gactaacatt gagatgaagc gtgcgtcgct ggcgcttcac aataaaccgc aacatctttt
    23221 tatgcgaaat atcaatgtga tgcaggaatc cactgttgga cccgcattga gcatgaactt
    23281 cgacatgcgc aaagacgtac gaggtgtctt tatggcgaaa aaagaaacac tgctatctct
    23341 tgcaaatgtt catgcggtga atgaaaaagg acaaagctcc gtcgatatcg acagggttaa
    23401 tcaccatatt gttaatgtgg aaaagattaa ctttagattg ccggaacgga gggagtagat
    23461 ttgcgaccat tcctggaaaa atggagccat acttaggaac aatgctactg caatccacaa
    23521 cgaagcggcg taacatcata agtaattcag taatcaattc agggtaattg atgctggcga
    23581 aaaaaatcga acaagctata attcagcaac cattttacag gtggatgaaa taatgacgaa
    23641 tttaaaagca gttattcctg tagcgggtct tgggatgcat atgttgcctg ccactaaggc
    23701 gattcccaaa gagatgctac cgatcgtcga taagccaatg attcagtaca ttgttgacga
    23761 gattgtggct gcagggatca aagaaatcct cctggtaact cacgcgtcca agaacgcggt
    23821 cgaaaaccac ttcgacacct cttatgaatt agaatctctc cttgagcagc gcgtgaagcg
    23881 tcaactactt gcggaagtgc agtccatctg tccgccgggc gtgaccatta tgaacgtgcg
    23941 tcagggcgaa cctttaggat taggccactc cattttatgt gcacgacctg ccattggtga
    24001 caacccattt gtcgtggtac tgccagacgt tgttatcgat gacgccagcg ccgatccgct
    24061 acgctacaac cttgctgcca tgattgcgcg cttcaacgaa acggcccgca gccaggtgct
    24121 ggcaaaacgt atgccgggcg atctctctga atactccctc tctgaatact ccgtcattca
    24181 gaccaaagag ccgctggacc gcgaaggtaa agtcagccgc attgttgaat tcatcgaaaa
    24241 accggatcag ccgcaaacac tggactcaga catcatggcc gttggtcgct atgtgctttc
    24301 tgccgatatt tggccggaac ttgaacgcac tcagcctggt gcatggggac gtattcagct
    24361 gactgatgcc attgctgaac tggcgaaaaa acagttcgtt gatgccatgc tgatgaccgg
    24421 cgacagttac gactgcggta aaaaaatggg ctatatgcag gcgtttgtga agtatggact
    24481 gcgtaaccag aaagaagggg ttaagttccg taaaggtatt gaagagttat tgagtaaatg
    24541 tattgtttaa caggtatact atattaatag agagtgcggt tgtccaaatt ttaggcacgt
//
LOCUS       DAJOWB010000065        24585 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJOWB010000065 DAJOWB010000000
VERSION     DAJOWB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.065"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..173)
                     /locus_tag="OHO85_002809"
     CDS             complement(<1..173)
                     /locus_tag="OHO85_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HCQ2672160.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     EYFRMSPEDWLRA"
     gene            complement(179..877)
                     /locus_tag="OHO85_002810"
     CDS             complement(179..877)
                     /locus_tag="OHO85_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCQ2672161.1"
                     /translation="MQDIQQETLNECTKTEQSALVVLWEIDLTEVGGDRYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFEMNGKGASARPTLKVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWIYRGDECGYDGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGYLSINKLSQ"
     gene            complement(877..1233)
                     /locus_tag="OHO85_002811"
     CDS             complement(877..1233)
                     /locus_tag="OHO85_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2672162.1"
                     /translation="MEDCTVPEPEVFNWIPREGMETTRKPSVITVKFGDGYEQRRAGG
                     LNADLKTFKPVFRVTDEYSRAALDSFLSRHAGIRAFLWRPPKHNRTVRVVCREWSISD
                     NAMYTDFNCTFEEVTH"
     gene            complement(1211..4438)
                     /locus_tag="OHO85_002812"
     CDS             complement(1211..4438)
                     /locus_tag="OHO85_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCQ2672163.1"
                     /translation="MATLRELIIKISANSRSFQSEISRASRMGQDYYRTMQNGGRQSA
                     AASREMRRALAEVTDQINTAKSSALNMAGAFAGAFATGHLISLADEWNSVNARLKQAS
                     QSSDDFQVSQRELMAISQRTGTAFSDNASLFARSAASMREYGYSSEEVLKVTEAISTG
                     LKLSGASTAEASSVITQFSQALAQGVLRGEEFNSVNENGDRVIRALAAGMGVARKDLK
                     AMADNGKLTADKVVPALISQLGALRDEYAAMPDTVSSSATKVENAFMAWVGGANEASG
                     VTKTLTGVLNGVADNIDTVAAAAGALVAVGVARYFGNMASSAGSATAGLITAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVVAARGTERQAAAEAKLTAAQASLTRNIAART
                     AAQTTLNTVTSVGSRLLSGALGLVGGVPGLVMLGATAWYTMYQNQEQARESARQYAAT
                     IDEIRQKTSAMSLPEASDNEEKTRQALDEQNRLIDEQKSKIKSLQEKIAGYQYVLANP
                     GWTTDNGFMINHMTSVKTVTEGLAEATNQLAVEQSRLTQMQGKAQSIQDVLAGLEERR
                     VALIRQQAAEQNKAYQSLLIMNGQHTEFNRLLGLGNELLLQRQGLVNVPLRLPQATLD
                     DKQQTALNNSERELALSRLKGEARERARLGYAADDLGFVGEAYQTARQNYINNSLDAW
                     RNNQANKPKAYKKTEAEKTEDIYKRLIKQQKEQIALAGQNTELAKMKYQVSQGELSTL
                     SEAQKKTLLQNAALIDQKKIREQLAAYESSLADSNASARASDEAQLLGYGEGSRMRER
                     LQEMWSIRQAFEQKNNELLRQYQAGEIEEALWKQEKELNKKYLEERLSDQQDYYAKAD
                     ALRNNWNAGLQEGLTNWADSATDYASQAADAVVSTMDGLVSNISDALAGNVVDWRNWG
                     SSVLREVSKILMNAAIVNGLKSLSGAGGWLGTVGGWISGAVANAKGGVYTSANLSAYS
                     NTIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAIGNVNGGGGFVYSPVY
                     HISIQNQGSNGEIDARSARGLVDLIDSRVVSIMQSSRRDGGLYSA"
     gene            complement(4485..4763)
                     /locus_tag="OHO85_002813"
     CDS             complement(4485..4763)
                     /locus_tag="OHO85_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004967194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HCQ2672164.1"
                     /translation="MMLALRMGRTLSELRREMSASEIMMWAEFDRFSPLGDERADIRA
                     AQIVSAVYGAQGVKVPLNDALLQWEKEQTEGVSDPFAGLENALLIVSQ"
     gene            complement(4787..5158)
                     /locus_tag="OHO85_002814"
     CDS             complement(4787..5158)
                     /locus_tag="OHO85_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HCQ2672165.1"
                     /translation="MTKNIRNLALATMSGFRHKTVDVPEWEGATVVLREPSAEAWLRW
                     QEIVKAKDDETPLSVAERARRNLEADVELFIDVLCDTGLQPVFSEDDREQVIAVYGPV
                     HARLLRQSLELISDAGEVKKK"
     gene            complement(5173..5877)
                     /locus_tag="OHO85_002815"
     CDS             complement(5173..5877)
                     /locus_tag="OHO85_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2672166.1"
                     /translation="MSSNFERSQLTKIMISSAPVTAETLDSASYLGLSCTIKEVQFTA
                     GQKQDIDVTTLCSVEQENINGLGAASEISMSGNFYLNAAQNALRSAYDNDTTYGFKVI
                     FPSGNGFTFMAEVRQHTWSAGTNGVVAATFSLRLKGKPVLTTEPLKVKVDLNSTLQVS
                     AGAKLEMVVEAAGGVPPYSYVWKKGSSPVSGQTAATFSKASAASGDAGAYTCEISDSA
                     SPVNKVTSTSCTVTVS"
     gene            complement(5937..6281)
                     /locus_tag="OHO85_002816"
     CDS             complement(5937..6281)
                     /locus_tag="OHO85_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HCQ2672167.1"
                     /translation="MRETTLYSLLSQLAGGQVYPYVVPLTEGKPAVSPPWLVFSVVSD
                     TASDVLDGQAESRITVQIDVWATVPDDADDIREQALDAVRQLAPSVISKTQGYDPDSR
                     LSRATLEFQVIA"
     gene            complement(6278..6724)
                     /locus_tag="OHO85_002817"
     CDS             complement(6278..6724)
                     /locus_tag="OHO85_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCQ2672168.1"
                     /translation="MDFSLDFSGLADIARDLETLSRAENNKVLRDATRAGAEVMRDAV
                     VERAPERTGKLKKNVVVLTQRSKRRGEIISGVHIRGRNLRTGNSDNSMKASDPRNAFY
                     WRFVELGTINMPAHPFIRPAFDTTEELAAQIAIQRMNQAIDEVLSK"
     gene            complement(6724..7062)
                     /locus_tag="OHO85_002818"
     CDS             complement(6724..7062)
                     /locus_tag="OHO85_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001147815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCQ2672169.1"
                     /translation="MQAGRLRDRVVILNATTVRSPSGHPVETMTEGATIWAEVKGISG
                     RERISGGAETAQATVRVWMRFRRDVTATSCLKVLTGAFKGAILSIDGPPIPDARATRL
                     EILCSQKGNV"
     gene            complement(7071..7376)
                     /locus_tag="OHO85_002819"
     CDS             complement(7071..7376)
                     /locus_tag="OHO85_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCQ2672170.1"
                     /translation="MMPTLEELRVQCRIDDDNEQENSLLMMYLAAAREEAEKFLNRTL
                     YDETVSEQDTTGLVITPLIKLRLMQLVGYWYENREMQDAVPDFFYTGLRMYRFHPGT"
     gene            complement(7388..7576)
                     /locus_tag="OHO85_002820"
     CDS             complement(7388..7576)
                     /locus_tag="OHO85_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672171.1"
                     /translation="MILKQDLKWSPDGMRVEIIRAGEYEDKELPERVREIATAAGIVS
                     DKRTPVARGADKSKKQHS"
     gene            complement(7627..8832)
                     /locus_tag="OHO85_002821"
     CDS             complement(7627..8832)
                     /locus_tag="OHO85_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCQ2672172.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVEAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEELDRNILNLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDARSQTATSKLGLIEPFMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTTGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATAVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDSEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            complement(8847..9497)
                     /locus_tag="OHO85_002822"
     CDS             complement(8847..9497)
                     /locus_tag="OHO85_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCQ2672173.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNALKNLNF"
     gene            complement(9475..10716)
                     /locus_tag="OHO85_002823"
     CDS             complement(9475..10716)
                     /locus_tag="OHO85_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCQ2672174.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADAIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRATGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPGCVVPKLNSSWEPVYQVTFPDGSTDVLS
                     QEDIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(10716..10898)
                     /locus_tag="OHO85_002824"
     CDS             complement(10716..10898)
                     /locus_tag="OHO85_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672175.1"
                     /translation="MFLIILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQPSVGGGK"
     gene            complement(10910..12667)
                     /locus_tag="OHO85_002825"
     CDS             complement(10910..12667)
                     /locus_tag="OHO85_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCQ2672176.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEERGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSSGIGLYMTGADGEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPRHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTLEAKIDGPVAMFTAMSRMLVNG
                     GEPEPDLSEHLVSVGIRSL"
     gene            complement(12667..13149)
                     /locus_tag="OHO85_002826"
     CDS             complement(12667..13149)
                     /locus_tag="OHO85_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCQ2672177.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDSKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRTETQ
                     SGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNIAGPDDVDPLAELLKARD
                     "
     gene            complement(13297..13647)
                     /locus_tag="OHO85_002827"
     CDS             complement(13297..13647)
                     /locus_tag="OHO85_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCQ2672178.1"
                     /translation="MPSRIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWDAIRARILKRDNHLCQNCLRNGRAVEARTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIN"
     gene            complement(13752..13934)
                     /locus_tag="OHO85_002828"
     CDS             complement(13752..13934)
                     /locus_tag="OHO85_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HCQ2672179.1"
                     /translation="MRKLKMKLCALMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPAP
                     DWQTPLNGIISPSESG"
     gene            complement(14151..14684)
                     /locus_tag="OHO85_002829"
     CDS             complement(14151..14684)
                     /locus_tag="OHO85_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCQ2672180.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(14740..15054)
                     /locus_tag="OHO85_002830"
     CDS             complement(14740..15054)
                     /locus_tag="OHO85_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCQ2672181.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
     gene            complement(15059..15274)
                     /locus_tag="OHO85_002831"
     CDS             complement(15059..15274)
                     /locus_tag="OHO85_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCQ2672182.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
     gene            complement(15567..15641)
                     /locus_tag="OHO85_002832"
     tRNA            complement(15567..15641)
                     /locus_tag="OHO85_002832"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15606..15608),aa:Gly,seq:tcc)
     gene            complement(15645..15720)
                     /locus_tag="OHO85_002833"
     tRNA            complement(15645..15720)
                     /locus_tag="OHO85_002833"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15685..15687),aa:Thr,seq:tgt)
     gene            complement(15723..15799)
                     /locus_tag="OHO85_002834"
     tRNA            complement(15723..15799)
                     /locus_tag="OHO85_002834"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15763..15765),aa:Lys,seq:ttt)
     gene            complement(15801..15876)
                     /locus_tag="OHO85_002835"
     tRNA            complement(15801..15876)
                     /locus_tag="OHO85_002835"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15841..15843),aa:Ile,seq:cat)
     gene            complement(16087..16776)
                     /locus_tag="OHO85_002836"
     CDS             complement(16087..16776)
                     /locus_tag="OHO85_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCQ2672183.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGSSIALVSQVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGIICWEYQIAITQWAWNEFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFQRLDSEALLFVMRTRSKQKA
                     AFSKQSVAKVD"
     gene            complement(16773..17138)
                     /locus_tag="OHO85_002837"
     CDS             complement(16773..17138)
                     /locus_tag="OHO85_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCQ2672184.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRHGNTYFISEAGKRYRHDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELECA"
     gene            complement(17139..18194)
                     /locus_tag="OHO85_002838"
     CDS             complement(17139..18194)
                     /locus_tag="OHO85_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCQ2672185.1"
                     /translation="MRILLRPVLVPELGLVVLRPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SEIVIHRYGTGAMVLCWHCDNQLRDQTSESLGQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAVCNQVADALPEAVLRRSLGLRAEKIRSLYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQNPPQEKTVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            complement(18196..18474)
                     /locus_tag="OHO85_002839"
     CDS             complement(18196..18474)
                     /locus_tag="OHO85_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672186.1"
                     /translation="MAHDTKLHNSDDSAVFASRHGRRSRTFKSDWFQHDPCTEEQAEW
                     LIHNYRRRGYEIKKALSLDYRHWIIYVRLPYSERPPRPSRTYQQRIWR"
     gene            complement(18771..19163)
                     /locus_tag="OHO85_002840"
     CDS             complement(18771..19163)
                     /locus_tag="OHO85_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672187.1"
                     /translation="MKKLVLVAGVMIATVMLGGCAAKVDPALKAEAMKPLTCNDEKQC
                     DFYWKRAQFWLANNSSWKIQTATDTLISTYNPSPNSPFLAYQVSKMPNEDGSSRIFIK
                     PFCDNMFGCQPNPYQAVVSFKNFVKTGQ"
     gene            complement(19307..19462)
                     /gene="hokD"
                     /locus_tag="OHO85_002841"
     CDS             complement(19307..19462)
                     /gene="hokD"
                     /locus_tag="OHO85_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCQ2672188.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(19699..20364)
                     /locus_tag="OHO85_002842"
     CDS             complement(19699..20364)
                     /locus_tag="OHO85_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCQ2672189.1"
                     /translation="MTIADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNECGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(20539..20964)
                     /locus_tag="OHO85_002843"
     CDS             complement(20539..20964)
                     /locus_tag="OHO85_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCQ2672190.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRLSGRETLRQLEARTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNVRYFRRY"
     gene            complement(20980..21750)
                     /locus_tag="OHO85_002844"
     CDS             complement(20980..21750)
                     /locus_tag="OHO85_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCQ2672191.1"
                     /translation="METVFDALKAMGKATSVELATRLDISREEVLNELWELKRNGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQEGAKTCEEL
                     AGKFGVSTRKVASTLAVLTATGRLARVNQNGKFRYCMPGDKLPAEPKSVPVTENDGKA
                     FPQPAGVALPVQEAATQEDIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            complement(21783..22325)
                     /locus_tag="OHO85_002845"
     CDS             complement(21783..22325)
                     /locus_tag="OHO85_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCQ2672192.1"
                     /translation="MGEIYSNRWTQKNGAAPSKLWIVQIGAMTEQQIRQVCRQCMDRC
                     RAGETWPPDLAEFVALISESGANPFGLTVDAVMEEYRRWRNESWRYDGSDKYPWSQPV
                     LYHICLEMRSKGIERQMTEGELKRLAERQLTKWAKHVSNGLSVPPVRRQLAAPKRPLG
                     PTPIELLKQEYERRKAAGFV"
     gene            complement(22237..23277)
                     /locus_tag="OHO85_002846"
     CDS             complement(22237..23277)
                     /locus_tag="OHO85_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCQ2672193.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEEVIDSILQAMQGRVLDGDLISGWEKRQV
                     LKEDNGNVSQTAKSPAERKRAQREREKLRKQNEGARDESRICHDMSRRVTTDKDTDKE
                     LNPTHNARVRESAPVSEANGVPLQTSEPDYLEGLSEPIGKFPMTDGWHPSPDFRRRAA
                     LWGVALPEPEFTPAELAAFRDYWAAEGKVFTQVQWEQKFARHVNHVRAQAKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFINDGNCLEAVGTHGGDLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            complement(23349..23774)
                     /locus_tag="OHO85_002847"
     CDS             complement(23349..23774)
                     /locus_tag="OHO85_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCQ2672194.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNIVVVH"
     gene            complement(23771..23998)
                     /locus_tag="OHO85_002848"
     CDS             complement(23771..23998)
                     /locus_tag="OHO85_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HCQ2672195.1"
                     /translation="MRVDELVQFFGSVQRVADFYGITREAIYMWRKRPGEIVPKGRAA
                     EAAAYSKGKLSLNPELYKKKDTTRDKRKSDS"
     gene            24098..>24585
                     /locus_tag="OHO85_002849"
     CDS             24098..>24585
                     /locus_tag="OHO85_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2672196.1"
                     /translation="MESLGIRLKKLRKDKGLTQVELGKLSGVTGVTIGYWEKDLNEPG
                     SKALSKLAQALGTTESYLLYGVSSPELSFVQSNPGTKIPYFSWGDAISFLILEGEKTM
                     GNVDRITTFFDVVEGDFAVSMPDDTMHNPSGSPSIPVGATVILRPGESYKNGSIVAVI
                     VP"
BASE COUNT         5670 a         6994 c         5606 g         6315 t
ORIGIN      
        1 tctgcccgca gccagtcctc cggcgacatc cggaaatact cctccggctc accggagata
       61 ttcacgcagg gaaaatatct ttccccctcc ggtgttctca ccacgaagcc gcacgactcc
      121 gctggcgcac atcgccgggc gtgcgccaga atcgctgatt ctgtctctgt catgggattc
      181 actgcgaaag tttgttaatg gaaaggtagc cgccaaaatt gccgacgtta ttgcggaact
      241 tgcaaccact caggcatttg ctgcacttat ccttcgtgat atcggacgtt ggctggtcat
      301 attcatccgc gaccgccggg ccatcataac cgcactcatc gccgcggtag atccaggtgc
      361 aggtgttggc cagcatgatg cgccccggaa aaacagcgcc atccgtttcc gtcggtgtgg
      421 acagcacaaa agaggcactg accgcgctca gttcgctgca ctgttcgatg cgccagcggc
      481 tgatcacctc ctgctccgga tcggcgtcac tgtttccgtt gacgaagttc accgcatcca
      541 gaaaacgggc gtaaacctta cgccggacca ccgttccgcc gaccagactc tgcagatctt
      601 ccgccatccc ggtgaccatg ccgtacagat tagagacttt aagcgttggc cttgcactgg
      661 ctcctttgcc gttcatctca aatccgcttc cctgaatggg ataagcctga tactgccgcc
      721 cctgccaggt gactggttca cctttttcgt tctgctcatt acagaagaaa taacgatctc
      781 cgccgacctc tgtcagatca atttcccaga gcacgaccag cgcggattgc tccgttttag
      841 tgcactcatt gagtgtttcc tgctgtatat cctgcatcag tgagtgacct cttcaaaggt
      901 acagttaaaa tcggtataca tggcattatc cgaaatgctc cactccctgc agacaacccg
      961 gacagtcctg ttgtgttttg gcggacgcca caaaaaagca cgaatcccgg catgacggga
     1021 taaaaaactg tccagcgcgg cacgggaata ttcatctgtg acacgaaata ccggtttaaa
     1081 cgttttcaga tccgcattca gaccaccagc ccgtcgctgt tcatatccgt caccaaactt
     1141 taccgtaata actgatggct ttcgtgtcgt ctccatcccc tcacggggga tccagttaaa
     1201 aacttcaggc tcaggcactg tacaatcctc catcccgacg cgatgactgc ataattgaca
     1261 caaccctgct gtcgatcaga tccaccagtc ccctggctga gcgcgcatct atctcgccat
     1321 tgctcccttg attctgaatg ctgatgtgat acacgggaga ataaacaaat ccaccaccac
     1381 cattcacatt tccaatggcc ctgaccccaa gagagccgtc cgctgcccgt gtcagtggca
     1441 tgattgcttc aggcccggcc tcgcccatca acccggcacc tttcgcaaaa gcaaaatacg
     1501 tcggtgtatc cacaatagtg ttactgtaag cactcagatt tgccgatgtg taaacaccac
     1561 cttttgcgtt tgccactgcc cccgaaatcc atccgccgac cgtaccaagc caccctccgg
     1621 caccggagag tgatttcagt ccgttaacaa tggctgcatt catcagaatt tttgaaactt
     1681 cccggagaac tgaactcccc cagttcctcc agtccacaac attcccggcc agtgcatcgg
     1741 aaatatttga taccagcccg tccatcgtgg aaacgacagc atctgccgcc tgtgaagcat
     1801 aatcggtggc actgtctgcc cagttggtca gtccctcctg gagtccggca ttccagttat
     1861 tacgtaaagc atcggccttt gcataataat cctgctgatc gctgagacgc tcttccagat
     1921 attttttatt cagttctttc tcctgtttcc acagggcttc ttcaatttct ccggcctgat
     1981 actgtctcag cagctcgtta tttttctgct caaacgcctg ccggatactc cacatttcct
     2041 ggagtcgttc acgcatccgt gagccttcac catatcccag caactgcgct tcgtcagatg
     2101 cccgggcact ggcattactg tccgccagac tgctctcata cgcagcaagc tgctcacgaa
     2161 tctttttctg gtcgatgagt gctgcattct gcaaaagcgt ttttttctgc gcttctgaca
     2221 gggttgataa ttcgccctga ctgacctgat atttcatctt agccagttca gtattctgcc
     2281 ctgccagtgc tatttgttct ttttgctgtt taatcagccg tttataaata tcttctgttt
     2341 tttccgcttc ggtcttttta tacgctttgg gtttatttgc ctggttattt cgccaggcat
     2401 ccagtgagtt attgatataa ttctgtctgg ctgtctgata cgcctctccc acaaagccga
     2461 gatcatccgc agcataaccc aggcgggcac gctcacgggc ttcccccttc aggcgggaca
     2521 gagccagttc gcgctcgctg ttattcagtg cggtctgctg tttatcatcc agggttgcct
     2581 gtggtagccg taacggtaca ttcaccagcc cctgtcgctg cagaagtaat tcattaccga
     2641 gcccgagaag gcgattaaac tcggtatgct gcccattcat gatcaacagg gactgatacg
     2701 ctttgttttg ttccgcggcc tgttgacgga tcaacgccac ccgtcgctcc tccagcccgg
     2761 caagcacatc ctgaatggat tgcgctttgc cctgcatttg tgtgagacgg gactgttcaa
     2821 ctgccagttg atttgttgct tctgcaagcc cttctgtgac agtttttacc gacgtcatgt
     2881 ggttaatcat aaaaccgtta tcggttgtcc agcccgggtt tgccagcaca tactgatagc
     2941 cagcaatttt ttcctgtaag gatttaatct tacttttctg ctcgtcaatt aacctgtttt
     3001 gctcatcaag tgcctgccgc gtcttttcct cattatctga cgcttcagga agcgacattg
     3061 ccgacgtttt ctggcgaatt tcgtcgattg ttgcggcata ctggcgtgca gattctctgg
     3121 cctgctcctg attctgatac atcgtgtacc aggccgtcgc ccccagcatg acgagtcccg
     3181 gcacaccacc aaccaacccc agcgcaccac ttaacagacg actccccact gacgtgacag
     3241 tattcagcgt tgtctgtgca gctgttctgg ccgcaatatt acgggtaagt gacgcctggg
     3301 cagctgtcag cttcgcttct gctgcggcct gcctttcggt accgcgagca gcaacaaccg
     3361 cctgttgcgc acgataaacc gccgcacgcg ccctggcggt tgctatctgt gtcccccgaa
     3421 gttgcgcttc agcaagagcc acttcgtttc tggctgcagt aattaatcca gcagttgcag
     3481 atccagcaga cgacgccata ttgccaaaat atcgggctac cccgacggca accagtgcgc
     3541 cagctgcagc agccacggta tcaatattgt ctgcaacacc attcaacacc ccggtgagtg
     3601 tctttgtcac tccgcttgcc tcgttcgcac caccaaccca ggccataaag gcgttttcaa
     3661 ctttggttgc agaggatgaa acagtatcag gcattgctgc atattcatca cgcaacgccc
     3721 caagctgact aatcagtgca ggaacaacct tatcggcggt cagttttccg ttatccgcca
     3781 tggccttcag atccttacgg gcaacaccca ttcccgcagc cagcgcacga ataacacgat
     3841 cgccgttctc attcacagag ttaaattctt caccgcgcag cactccctgc gccagtgcct
     3901 gactgaactg cgtgatcacc gaactggctt ctgctgtact ggcaccggat aatttcaggc
     3961 ccgtggagat cgcctcggtg actttcagta cctcctcaga actgtagcca tactcccgca
     4021 tggaagctgc agagcgggca aaaaggctgg cgttatcaga aaacgccgtt cccgttctct
     4081 ggctgattgc cattaattca cgttgtgata cctgaaaatc atcactggac tgtgaagcct
     4141 gcttcagacg ggcatttact gaattccact catcggcgag agaaataaga tgaccggtag
     4201 caaaagctcc ggcaaatgcc cccgccatat tcagtgccga agatttagct gtatttatct
     4261 gatccgtcac ttctgccagt gcacgccgca tttcacggga tgcagcagcg gactgccggc
     4321 ctccgttctg catggtacgg tagtaatcct gccccatacg cgaagcccgg gagatctctg
     4381 actggaatga ccgggaattt gccgagattt taataatcag ttcacgtaat gtcgccacac
     4441 tcattctccg gacgaaaaaa aaccgccgaa gcggttatgt tgactcactg agacactatt
     4501 aaaagcgcgt tttccagtcc ggcaaatgga tctgatacgc cttctgtctg ctccttctcc
     4561 cactgaagaa gcgcatcatt cagtggcact ttgaccccct gcgcaccgta aacagctgaa
     4621 acaatctggg cagcccggat atcagcccgt tcgtccccca gcggactgaa cctgtcaaat
     4681 tctgcccaca tcatgatttc tgatgctgac atttcccggc gtaactctga caatgtgcgc
     4741 cccatcctga gcgccagcat catcagaaaa cgcatccccg gaagcgctac ttttttttaa
     4801 cctcgccggc atcactgatc agttccagag actgccgaag aagccgcgca tgcaccggac
     4861 catacacggc aatcacctgt tcacgatcat cctctgaaaa tacaggttgc agtccggtat
     4921 cacacagaac atcaatgaac agttcaacat ctgcctccag atttcggcgg gcgcgctccg
     4981 caacggataa cggtgtctca tcatcttttg ctttaacgat ctcctgccag cgcaaccagg
     5041 cttctgcaga aggttcccgt aatacaaccg ttgctccctc ccattcaggc acatcaacag
     5101 ttttatggcg aaaccccgac atcgttgcca gtgccagatt gcggatattt ttagtcatca
     5161 catctatcct cattaactga cggtaacagt gcaggaagtg gaggtcacct tgttaacagg
     5221 gcttgctgaa tcagaaatct cgcaggtata cgcaccggca tcacctgatg ctgctgatgc
     5281 cttactgaat gttgccgccg tctgtccgga aacaggagaa ctacctttct tccagacata
     5341 agaataaggc ggcacaccac cggcagcctc aaccaccatt tcgagtttcg ctccggcaga
     5401 aacctgcagc gtgctgttta aatcgacctt cactttcagc ggctctgtcg tcagcacagg
     5461 tttacctttc aggcgcaggg aaaacgttgc agccacaaca ccattggttc ctgcagacca
     5521 ggtatgctga cgcacctctg ccataaaggt aaatccgttt cctgacggaa aaataacttt
     5581 aaagccatac gtggtgtcat tgtcataggc actgcgcaac gcgttctggg cagcattgag
     5641 gtaaaagttg cctgacatgg aaatctctga cgcggcacca agaccgttaa tattttcctg
     5701 ctcaacagaa cacagcgtgg tgacatcaat atcctgcttt tgtcctgcgg taaactgcac
     5761 ctctttgatt gtacagctca gaccaagata gctggcagaa tccagggttt ctgctgttac
     5821 cggtgcagac gaaatcataa ttttcgtcag ttgcgaacgc tcaaaattag aggacatact
     5881 cgtctcctga aaataaaaaa cccgccagcg gcgggtgggt aaaatcatta acgacctcag
     5941 gctattacct gaaattcaag cgtggctctg ctcagacggg aatcaggatc ataaccctga
     6001 gttttagaaa taacggaggg tgcaagttgc cttaccgcat caagcgcctg ctcacggata
     6061 tcatctgcgt catcaggtac tgttgcccag acatcgatct gcacggtaat tctggattca
     6121 gcctgaccat caagcacatc agacgcagtg tcagacacca cagaaaatac cagccatggc
     6181 ggagataccg caggctttcc ctccgtcagc gggaccacat aaggataaac ctgtcctccg
     6241 gccagttgag acagcaggga atacagtgtg gtctctctca tttacttaag acctcatcaa
     6301 tagcctgatt cattcgctgt atggcaatct gtgctgccag ttcctctgtc gtatcgaaag
     6361 ccgggcgaat gaacggatgc gcgggcatgt ttatcgttcc cagctccaca aagcgccagt
     6421 aaaacgcatt tcggggatca ctggctttca tgctgttatc actgtttccg gtccgcaggt
     6481 tccgtccacg aatgtggaca cccgagataa tttccccccg acgctttgaa cgctgagtga
     6541 gaacaaccac atttttcttc agtttcccgg ttcgctccgg cgcacgttca acaactgcat
     6601 cccgcataac ttcagcaccg gcacgggtgg catcgcgcag taccttattg ttttctgccc
     6661 tgctgagcgt ctccagatcc cgtgcaatat ccgccagacc tgaaaaatca agactgaaat
     6721 ccatcacaca ttccccttct gagaacagag tatctcaagc cgtgtggcac gagcatccgg
     6781 tatcggcgga ccgtctatac tcagaatcgc gcctttgaat gcaccagtca gcactttcag
     6841 acatgaagtt gctgttacat ctcgccggaa tctcatccag accctcactg tagcctgagc
     6901 agtttctgcg cctccggata ttctctccct gccactgatc cccttaactt ctgcccatat
     6961 ggttgccccc tccgtcattg tttccacagg gtgccctgac ggagaccgaa cggtggtggc
     7021 attcagaata accacacgat cacgtaatct tcctgcctgc atgaatcctc ctatgttccg
     7081 ggatgaaatc gatacatccg cagtccggta tagaaaaaat caggcactgc atcctgcatt
     7141 tccctgttct cgtaccagta gccaaccagt tgcataagac gcagttttat cagaggtgtt
     7201 attacaagcc cggtcgtatc ctgctcagaa acagtttcat cgtaaagcgt ccggtttaaa
     7261 aacttttcag cctcttccct ggcagcagcc agatacatca taagaagaga attctcctgt
     7321 tcattgtcat catcaatccg gcactgaaca cgaagctctt ccagagtggg catcatttgg
     7381 gcaacctcta tgaatgctgt tttttagact tatcagcccc ccgcgcaaca ggtgttctct
     7441 tatcagagac aatcccagct gcagtggcaa tttcgcgtac ccgttcgggt aattctttat
     7501 cttcatactc accggcccga ataatctcaa cacgcatacc gtccggtgac catttcagat
     7561 cttgtttcag gatcatgatt ctttcacctg tcagaacagg ggcgcacttc tgcgccccct
     7621 gaatgattac gctgctgcaa tcttcagcag tttgatggcc tgcgaatcga ccagcatccc
     7681 gccggtgcgc ttggtggtat aaaaaccgac aaacggttta ttggtgtacg ggtcacgcag
     7741 aatgcgggtg ccgatacggt caacgatggt gtaaccccgt ttgaagttac caaatgcaat
     7801 ggctttcgca tcagcggcga tatccggcat ctgttcgttt tcagcgatac cgtaacccgc
     7861 cagagaggac ggctgcccca gctccagccc cggacgccac agatagttac cctcgctgtc
     7921 tttaagcaga cggatggcaa acaggctgtt gttgttcatc atgaacttcg cgccagtgcg
     7981 gtgtgcctta cgcagcgtgt aaatcagttt gataatggca tctgcggtca ctgccgtcgc
     8041 ttcgccggat acaatatgct gaagtttgcc gaacgcccgg accttgtcgg tttcatcagt
     8101 ggactcatac gccaggaacc ctttcggctt cttggtacca tcgccggtgg taaaggcaat
     8161 ttcttcctgt tcggcaaatt cggttgccag ctcgctgttg atccaggcct ccacgttgaa
     8221 gaaagcatca tccagcattt tctgggtggc ctgcgggtta ccgtagattt cccccatgaa
     8281 aggttcaatc agtcccagtt ttgaggtggc agtctgggag cgcgcgtcag tctcgccaac
     8341 ccatccggaa gccgtgccgc ccagattcac cagttttttg tagtcggaac cgccaacggt
     8401 gatcaccgtg gcttcctggc gcatcaccac ttcatctttc agcagattga gaatgttgcg
     8461 atccagttct tccggcacgg catagccgcc gtcttcatcg gtgcccacct gtaatgcctt
     8521 acgctccaga tcgcgcagac catcttcacg gcctttacgc aggaagccca caaacgcttc
     8581 tttatgctcg gtggccagtt tattttgcgc accacctgcc ggacgtttca gctcaagcag
     8641 ctctttttca agatcgcttt tgagattttc cagctcgctg agttttccgt tcagggtttc
     8701 cacctgcccg gcaagtttgc ctttttcctg ctcaatcgcc tcaacgcgct tgtcgttctt
     8761 tgctttgaag tcgtcaaact tctgctgcag ctcctgcgcg acctgttcga catctttaat
     8821 atcaaccgcc atcgtatttc tcctgattag aagttcagat ttttcagtgc attcagtgca
     8881 gagcccacat cctcagcgtc gcgcagggac agtgcgccat agccccctgc catgaatgct
     8941 ttggcctggg tacgggagag tccgacatca cgcaggactc tttcgatttt tttctgttcg
     9001 gggatttccc cgcgggccag tgcgttcttg acgtcgctga tccgcgcctc gtcgttagac
     9061 gggaacgtca ccaggctgac ttcccagagg tcgatttctt tcagcagaaa ggcttctttg
     9121 ctccggtcgt attcccagtc tttcaggacg tacccaatag aaaggccggt taacgaaccg
     9181 gccttcatgt gtgcatgtgc gcgttttgcg aggggatcat catcaataag caaccgtccc
     9241 ctgacgtaaa gcccgacatc gtcttccttc atttcggtgt aaacaccgat gggttcatcc
     9301 atgcggtgct gccagagcag cgcaggtaac gcttttctgt cactccacgc ccgcagggaa
     9361 gcagcaaatg ccccggacat caccacatca tcgtggctgt cctttacacc aaagacggag
     9421 ccataccctt caaactcacc ggagtcactg acagatttca gactcagcgg tacatcaaga
     9481 cgttgtttcg tctgcattgg cgttatcctt ctgcttaccg gctttactgc catcggaggg
     9541 tttcgtggtc atgttcatcg gggtgagata gacatcacca ccgggacgcg gattcatatc
     9601 ttccaggtcg cggcagtcat taggagagta aattccccag ttgatcccgg tggcgtaggc
     9661 ttcaaaacgg gacttcatat ccccgcgcag taacgccccg gcgttaaatt tggcgtaata
     9721 aacgccctgc ttactttttc gtaccagtcc ggtgttgatc cgctgctcaa tgcgggtcag
     9781 atacggcacc agtgaatagt tgataaatcc cagccccagc tcttcgatat tgttgaaggt
     9841 ggcgcgatcg gtgttctgca ccatgtgcaa cggcacccga aacagacgac agatttcttc
     9901 aagctgaaac ttgcgggttt ccaggaactg gctgtcctcg gcgttcagcg ccatcgactt
     9961 ccagtccagc cccatctcaa ggatcatcgg gcggtgagca ttgccaagcc cggtgtgacg
    10021 ctcctcaaaa tctttcttca ggcgctcgta agcctgatct gacagcgtct gctctgtacg
    10081 caacacaccg gacgtcaccg caccattgct gaacagtctg gccccgtgct cttcggtcgc
    10141 tgccgccagc gatattgcct cgcgggcata ggcgatggga ttcagcccca ccagtccgtc
    10201 cagcgtcagc gtgcgcacat gccagatatc ctcctggctc agtacatccg tggagccgtc
    10261 cgggaatgtg acctgataga ccggctccca gctactgtta agcttcggta ccacacagcc
    10321 aggatcgacg ggcagcagtt cagccacttc gccaaatgct ttcactttgt aggcgtaaaa
    10381 gtttcccctc aggcacagac aggtgaccac cagctcccag aactcctgcg gcgtcatata
    10441 tccattggga tgcgtggaga tcagcttatg cagacgttcg ccggtggctc tctgtttcag
    10501 gctgccgttc aggtgataca gattgcaggg caacatcccg accgactctg ccagcaccct
    10561 gacgcaggaa aaaaccgccg tcagtcgcat ggcccgctgg ctgctgatct gctttccggt
    10621 ataggtgtcg tatgacagcc cgatagcatc cgccagctct gctggcgtgg tcaccggcgc
    10681 gtcacttttt cgttgaaata atcccgaaaa gaacactatt tacctccgcc gacagacggc
    10741 tgtgtacggt cgagatatcg cgccaccagc cacgaccaga acaggcacag cgccccggca
    10801 acaacaaaac ccgccggggg ataaatcagc caggcaccat acgccagcaa aagcgcaccc
    10861 agcacgccca ccagtggcgc gagaattatc agaaacataa tgacctcggt taaagcgagc
    10921 ggatgcccac gctgaccaga tgttcagaca gatccggctc cggttcacca ccattgacca
    10981 gcatccggct cattgctgta aacatcgcaa cagggccgtc gattttggct tccagcgtgg
    11041 atttattcgg gaagatattg tcgtttttgt ccggttttac cgtaacgtta gacatcatcc
    11101 agttcatgac cggatgattg ctgtgatgga aacgtccggc atagaccagt gattccgttt
    11161 ccttcatggc ctctgacaga ttgcgaaccg tctgcggaac ctccaccagc ggtatccctt
    11221 cttcagccag tgctaggctg aactgcatcg cgctccacgg gtcaaatccc agttccctga
    11281 ggttttcacc accaatccat tccagtaagt cactttttat ctgagcatga tcgataacat
    11341 caccatccgt cagaatcagc ttatccatct ccgcccactt ccggtaaagt tctgcctgct
    11401 gccgcgagca tcgttccagc cgtccttccg ggagccagaa tttaaaatcg gcatgaacat
    11461 gcccgttatc cgttcgccag agttttgccg ccgcacagat atcaatctta tgagcaaggt
    11521 caacgccgac ccacatggga tacgttttca gctcatgtcg cggggcaatg tattcgcact
    11581 tctcccactt aatcatgtcc atccaggcag actctgctgt tacccacaca ttcatgtgtt
    11641 tggtaaaaaa attcacccgc gcagagacct gttctttcgc ttttttcgcc agacgacgca
    11701 gatcatccca gcgtttacag atgcccaggc caggattcgc tttctgccag accgtttcat
    11761 caaacggatc atctccctca tcgagggtgt aaataatcgc aaagtaggag tcgtctttta
    11821 cagcgccctc cacgtcgctg ttatagccac gcaatacctt gatggcataa tcacgctgct
    11881 cgtaacaaat cccttccttg ttaaaccctg ccgtggtgat accaaataaa agggactgca
    11941 gacgggcacc ggttgccgtt tccagaacgt cccacacgtc acgggtttta tgtgcatgca
    12001 gctcatcaat aatggcgcag tggatgttca gaccatccag gttgtttgca tccgaagaaa
    12061 gcggttcaaa ttttgatgcg ctctgctcct ggtaaatcgc cagcttgttg aaatcaaaca
    12121 accgcccgag tgtcgaccgg gcttttctga ccatattttt ggcgtcttca aacacgattc
    12181 tggcctggtc acgcgtggtt gcggctgaat acacctcagc accgccttca ccatctgccc
    12241 ccgtcatata cagaccgata cccgatgaca gggttgattt tgcgttttta cgggcaactt
    12301 cgttgtatgc tgtccggaac cggcgcacca tcaccgggcg cccgctgcca tcgctgcgca
    12361 tgacaacttc cccggtctct tcattgacca gcggaatgac aaaaccaaaa atattaatga
    12421 ggataaatac atgccagtcc atcaactcaa taggctggcc tgccagcgcc ccttttacat
    12481 gaggcacaaa tttgtagaaa ttcaggatgt gctgcgcacg tggttcactg aaataaatcc
    12541 cccgctcttc gccgtacttt agatcatcaa gaaaacgctg gcaggccagg cggacaaatt
    12601 cgccagcaac aatttctcct gcaacaacac gttcggcgta gcggatcccg tcagccactt
    12661 ttgccatcag tctctcgctt ttaaaagctc cgccagcgga tcaacatcat ccggtccggc
    12721 aatatttact ttagcccggc ttgccggtga cataccaaac tctgcaagca ttgcccggat
    12781 ccgcttccag gcatccgctt tcattgccgc cgcggggtgc gccttaatca gtacatcacc
    12841 gctctgcgtt tccgtgcggt aggtataccc ctcaacatcg agtgtttcgc agtgatgccg
    12901 atattcggta taggcttcca ccagcaactc gagtgcacgc gcatcaagct gagaaatgat
    12961 cccttccgca ttcagctctt ccgccattcg cctgaaccag tacttcccct gtgcccctaa
    13021 atgctgcgga attttaggga gacctttttc atccttttta gcggtttttt ttgagtcttt
    13081 aacggggcgc tttgaggggt tgcctcgtat caaatgcagg cgtggcgggg ttttcggagg
    13141 tcctgacata atcggtctta cctatcaatc gtttgttcac atttccaaaa aaagttttcg
    13201 aacctgcggc gatgtgagga agggtcaggc ggcggtactg agcagccagg gttgcagaga
    13261 tttgacccgc ccctccccta cagatgggaa ctgttatcaa ttgatgcgtt cgcgcgctgt
    13321 ttttgcttta tgacagggcc agcacagact ctgcaggtta ctgtctgcat ccgtgccacc
    13381 atgagctttc ggaatgatgt ggtccacagt tctggcttca acggctctcc cattgcgcag
    13441 gcagttctga cacagatgat tatcacgctt cagtatgcgc gcacgtatgg catcccattt
    13501 cgagccatag ccacgctggt ggcggctcag tccgcgttga tgctgtaccc atccttcgcc
    13561 acgatgttta tcgcagtaac cagaactgtc tgtggttgta cctgcacatc cacgcttacg
    13621 gcaggcgcgt gggattcgtg atggcataaa tacctcatac cctgcgaaat gtttaccacg
    13681 ataaaaaggc tacttaatgc actgagtgcg gatatactcc tgtgcccctt ccagttgcat
    13741 ctgcatcgtc atcagccgct ctctgagggt gaaataatcc cgttcagcgg tgtctgccag
    13801 tcgggggctg gttgcattat ccacgccgga ggcggtggtg gcttcacgca ctgactgaca
    13861 gactgctttg atgtgcaacc gacgacgacc agcggcaaca tcagcgcgca gagtttcatt
    13921 ttcagctttc gcattggcta attctctcga gtactttgca tcgagcgcag caacatcacg
    13981 ctgacgctgc tgcatgtcag cgatggtggc gatcgcctgc ttcagctcac tgactttttt
    14041 atcacgctgt tctttgtagg cgatggcgtt atcacggtaa tgattgaccg cccacgacag
    14101 gcagacgatg atgcaaataa ccagagcata aataatcgcg gcgactctgc tcactgatct
    14161 atcccccaac aggctaatgc gctttcctgg tcacgacgaa taacctgtcc atagcagtta
    14221 tttgaacgta tgcggcaatc gcgcccacca tcttttatcc accagcgaat cgcctcgcat
    14281 gcgcccttac gatcaccggc attcagccgc ttataaaacg tcgacgggaa acacttaccg
    14341 gggccaatgt tatagggaca gaatgacgct atacccgctt tctgtggttc ggtcagtggt
    14401 actttaatat tgcgctccac ccatgccagc gccttatcac gttcaatagc gttaacctgg
    14461 tcgcattttt ccttcgacag tttcattccc ggtatgacgg gcttaccatc caccattgtg
    14521 gcaccacgac agatggtcca tatgccggaa ccatcgcggt atgccgtagt gtggttaccc
    14581 tctttttcat ccagaaactg gtcaagtatt tgaggagcag acgcgcctgc agcaatcagc
    14641 gccagaaccg cagctgacag gccgtatttg atttttgcgc tcatggatat ttatcaggat
    14701 gctaccaatg aaagatactg gaaagccaac tgcaaaaagc taacaacctg taatcgagtt
    14761 atcagaactg ttaattttta tggtataccg cgcctctgaa caggggcgcg tttctggcaa
    14821 cagctcgtcc ccttcacata acccggcagc aacatccagg aagacctgtc tgatgctcct
    14881 tctggctgct gcctcataaa actccagcgc tgcaccttca acacggtcca gcgagatgtc
    14941 caggtcaaaa atttcaccgt caaagcgttt tttgtcccgt aacgctaaag ttaccgtaac
    15001 tttattctca aaattgcgga tccctttcac aatcagttca tagttttgag tcattgaatt
    15061 actctccccg tgcagcctta cgcttgtctt ctctgatttt gaagtacaga tttgtcagat
    15121 aagtcaggaa gcccagaacc agactcccca gaacaccaat cgcagcccac tgtgacggac
    15181 tgacctgatc aagccactgt aaaaaccagt agccagcact gcctgcggag gtgccgtagg
    15241 caatgcccgt tgaaattttg tccatggatt tcatagcctc acctccgcaa ataacggatg
    15301 gcgtagtttt acactgagaa atgaaaggga tttgaaaaga aaaaaccgca aaagcgggcg
    15361 aaacgatata tacagaaagg aaagcactct atccaacaaa ccacccacag ttgatcggaa
    15421 taaaagcaga gtgcttatga atgatcgcct gcccgaaggt tagtatttct gcacagcaat
    15481 tttgcaaaaa aaggcgatca ttcataactt aaacgtcttt cagtcactcc gggatttccc
    15541 atcatcgcag actgaaagac tctgactgga gcgggcagcg ggaatcgaac ccgcatcatc
    15601 agcttggaag gctgaggtaa tagccattat acgatgcccg catatggtgc cgactaccgg
    15661 aatcgaactg gtgacctact gattacaagt cagttgctct acctactgag ctaagtcggc
    15721 actggaccgc caccggggac tcgaacctcg cacactcaac ttaaagggtt gacgctcttt
    15781 cctgatgagc tagtggcggt tggtggccct tgctggattt gaaccagcga cctggcgatt
    15841 atgagtcgct cgctctcacc actgagctaa agggccgggc gcaggataat aacgttacga
    15901 aatcaatgtt gcaagcattc aaaaatcacc tggttaaaaa tcacccttag ctcctccacc
    15961 agcgcattca ccatgtctat ccgagataag tggcacaaaa aaacccgctt gtgggcgggt
    16021 tttgtttgct tttgccatca cgtacaaaat cggcaaaata tcagatttgc atgaaatata
    16081 tgcctttcaa tctacttttg caacactttg ctttgaaaat gccgcctttt gttttgaacg
    16141 tgttctcatt acaaacaata aagcctcact atccagtcgt tgaaaaatgt gtttcattgc
    16201 aacccagtga cgagtaaatg ttttggacca gtttttagtt gtcactcccg ccagtaatgc
    16261 cagctcctgg tattcataac cttccccacc aaaaagttct gcttttactg cctgcgccgc
    16321 cagccagatt aattttttca ggcgttcctg cgttttccct gcaatttttc tggtaccgga
    16381 ttgagtatta aattcattcc acgcccactg tgttatcgcg atctgatatt cccaacaaat
    16441 aatcccgctg taacaccaca acaaccaggc tttatgatgt tcttcaagag acagaactgc
    16501 ccgtcgccat gatgatgtcg aaaactcaac ctgacttacc agagcaattg atgagccttt
    16561 cgccagtgat tgttttcccg gtatcggtgg attatcccgc gttatcattt ttccagtcac
    16621 ttcatcgcgg taccggattt tttttcgcct gtaacgccct gtatcgaaca tggcattctc
    16681 ctgccaggct tcaagctgac cttttgttgc cccactcaaa tcagcggtgg cgataatgag
    16741 ctgctcacgc acaaactgta aatactggtt attcatgcgc actccagttc tgtgattttt
    16801 atccccagcc gcccaccagg aacgagctga ccgcgcacaa tattgatttc atcaaactgc
    16861 tcgtcgtcta tgagtagtcc ggcatgcgtc agcgcatcca gtggtgcttt caggatattg
    16921 tccaggtcac gacggcgctt atccggtggc tctgcaataa tctttatcgc cagccttccg
    16981 gacaggttta atttcaaccg ctgctggcga acaattagcg ccacatcatg gcgataacgc
    17041 tttccggcct ccgagatgaa atacgtattg ccatgacgtc gccagtaggt attcaccgtc
    17101 ggcgggtaag gcaaaacaaa ttctatgcgt tcagtcattc atgctttcca cttcaggaca
    17161 cccgaatttc tcgcgtgcat taaaaaacga atcagcaaca acagctggct gccgtgtttt
    17221 tcttcaaaat cttttacccc ggcgtgcagt tcgttatgac atttacggca cagcggaata
    17281 acaaacaaat catcagcctt tgttcccatt cctcccagtc catgaccaat gatgtgatgc
    17341 ggatcatctg cctgattacc gcacgtcatg catttctgcg tttttaccca acgcgtgtat
    17401 acaggcatct cttcccgttg tggtttctgg cgctggagat actgagccgg tgactccgga
    17461 tcaacggcaa tgctgaccac cgtcttttcc tgtggcgggt tttgctggtg ggcatgaggc
    17521 aacggcgcaa gattttttgt gcgctgcttc agtatgctgg tggcggtctg ctctcccggt
    17581 acgatgtcgc tttcgcggta caaggagcgg attttttccg cacgtaaccc cagagaacga
    17641 cgtaatactg cctccggaag cgcgtccgcc acctgattgc agaccgccca ccaggataat
    17701 tcagccagcg acaattcccg ctcctgcgtg ccattcattg catggcgtat gacgtcaatc
    17761 atccatgctg acaggttttg atgagcaagt tgcccgagtg attcggatgt ctggtcacgc
    17821 aactggttgt cgcagtgcca gcacaacacc atcgcgccgg taccgtaacg atgtatgacg
    17881 atttcactgt gatgatagtc accatgaggc cactggcagg atttgacatg acgcaacagc
    17941 cagtcagaca gtgccccagc gccgccagca gcacgaatca cccgctcatc gctgaaaaat
    18001 ggcagtaatg atttatcctc cgccagcggc tggcgaacgg cagggacgac tccggacggc
    18061 agaccgcgca tgcttttcgg ttcaggctcc accagcactc gagggttatg aaatacttgc
    18121 atggattcac ggcccggcct aaggaccacc agcccgagtt ccggtaccag aacaggtcga
    18181 agtaatatcc gcacgttacc tccagatccg ttgctggtat gtgcgggatg ggcgcggtgg
    18241 gcgttcggaa taagggagcc tgacatagat tatccagtga cgataatcga ggctgagggc
    18301 tttcttaatc tcgtatccgc gtctgcgata gttatgaatt agccattcgg cctgttcttc
    18361 agtacatggg tcatgctgga accagtcaga tttgaaagtg cgggaacgcc gcccgtgcct
    18421 gctggcaaag acggcagaat catcagaatt gtgtaatttg gtatcgtgcg ccatcggttg
    18481 tctctgctgg cgcagcaggt gccagttgtt caggctggcg tgcgaattgt aaaccagaat
    18541 gccaggaaaa aacaaaaccc gccgaagcgg gtatgctaaa acaaactgaa agtaatatac
    18601 cggacttgta aaggaacgat agaataatta ttggattaaa ccctgactca atccagattt
    18661 tataggcaac aactacggac taatcatcac agtcatgttt gataggctta gtccacattg
    18721 ggtgagggtt tacggcgttt tcactaataa tttatcgtcc aaactataca ctactgccct
    18781 gttttaacga agtttttaaa ggaaacaact gcctgatagg ggtttggttg acagccaaac
    18841 atattatcgc aaaaaggctt gatgaaaatt cttgaggatc catcttcatt tggcatttta
    18901 ctcacttgat aagcgaggaa tggactattt ggagagggat tataagtgga aattagcgtg
    18961 tctgtcgccg tttgaatttt ccatgaggaa ttattagcca accagaattg cgctcgtttc
    19021 caataaaagt cacattgctt ttcatcatta catgttagtg gcttcattgc ttctgctttc
    19081 aacgctggat cgacctttgc tgcacaccct cccaacatta ctgttgcaat cattacacct
    19141 gcgactaaaa caagtttctt catctccctg ccccatcaat aaaaattcgg ttctctaata
    19201 actagagtta atcaacggaa aaaacgccga agcgggttaa gtgcgggtgc gttgaggatg
    19261 cctgacacat cagaggtggc gagggatttc tcccccgcca ggtctcttac tcctcaggtt
    19321 cgtaagctgt gaagacagcg acctccgtct ggccggttcg gattcgtacc tcgcagaggt
    19381 ctttcctcgt taccagtgcc gtcacaatga cggttaaaca gatgacgatc agggcgatta
    19441 acatcgcctt ttgctgcttc atagcctgct tctccttgcc tttcggcacg taagaggcta
    19501 acctagattt gccgttcata gattgagcct cagattaatg ttaagcgtct tgcaggacgc
    19561 gtaatgttaa ctggggcttt tctctatctg cctttggtgt tcatgcctga gacagatagc
    19621 ctcaagcacc cgcagccatt ctacttaact cccgttacct cgccaatata aaatcaatca
    19681 gaaaggtgat ccataaaatc actccttctc ttcttttccg tagtggagtt ggccaatttt
    19741 gataagaggg cgtccctgag atttgcggtg tagattggta tcgcgcagag aatacacaca
    19801 gccacaatat tcctgctgat agaatttttc gcgcttgctg atttcaatca tacgggacga
    19861 gccgccctgc ttgcgccagt tataatccca gtacaccata cccggataat gcgcaacagc
    19921 tcgccgccca cactcgttaa cctgctgcat atttttccag cgtgaaatgc ccagtgaact
    19981 gctgatcaca ctgaaaccat tttcagcagc gtacaacgct gtccgctcaa aacgcatgtc
    20041 aaaacacatg gtacaacgga tccccctctc aggctcccat tccattcctt tggcacgttc
    20101 aaaccagttg tcggtgtcgt aatcagcatc gataaacggc acgccgtgtt gttcagcaaa
    20161 gcgaatattt tcatccttac gaattaaata ctctttctga ggatgaatgt tcgggttgta
    20221 gaaaaagatg gtgtagtcga ttcccgaggc ctgaagcgcc tccatcactt caccggaaca
    20281 tggagcacag caagagtgca gtagtagttt gtttgccccg tttgggagct ccaatttagg
    20341 ccgtttgaaa tcagcaatag tcataaatat ttttattggg gtcatgaaaa tagcacagag
    20401 tgtagcatca gagcaggtct atcgggaata tatgtctaaa tctggtaata tctggttttg
    20461 acgcaaagcg gacaaccacg ctggctctac cctgcgccat gaaaatgtca attcacatct
    20521 gaactaatgc tctttaatct agtaacgtct aaaataccta acatttcctt gataaaatgc
    20581 cagtacacgc tgcatagctt cgctcttccg gcactcgcga cagattatat tcaggcgcct
    20641 gtcgtagcgg cgtatttcgc cgtctggtaa cgaccagata aggtccggat caaccactgc
    20701 aggtttcttc acctttgccc ttgagagttt tttgcgagca ttttgccagt ccttacgcgc
    20761 ctgttcagac gggaataacc cgtaaccaga gttgtataca tcgccactgg caaccagctc
    20821 tctggcgaga acactcatca gatatcttgt cgcacctgtc ctggcttcca gttgccgcaa
    20881 cgtctcgcga ccgctcagac gtacaagttc aacaacctgc cctttaattt tttcccgctc
    20941 ttcttgtgta aatacttttg ccataagcgc ctccggcaat cacttttccg atacaacacg
    21001 gcgggaagaa tcagtaatct gtcgaacaat atcccggtgc ttgttcagct cccgcagcgc
    21061 ggcgcagact cgctcccact tctgaacatc acttttcgcc ctgcgcagcg ccaggtttgc
    21121 cctgcgaagg gacggaaaaa tcagctcatc tgcttgcgtt tcggtaaacg atggcaacgg
    21181 ctgcacaatg tccgccacag tttctgtttt aatatcttcc tgtgttgccg cttcctgtac
    21241 tggtaacgca acacctgctg gctgaggaaa ggccttacca tcattttccg ttaccggcac
    21301 ggatttcggc tctgctggta acttatcgcc cggcatgcag taacgaaatt taccgttctg
    21361 attaacgcgt gccagccgcc ccgttgcggt taacaccgcc agcgtggaag caaccttgcg
    21421 agtactgaca ccgaacttac ccgccagttc ctcacacgtt ttagccccct cctgaccgat
    21481 aaactcaatc atcatgtctg cggtaacttt ttgttcgacc tccccggtca gcatatcctg
    21541 tgcttcagat tttactggcc gctcttcggt tacccgggat tcaccttcgc cagccagaaa
    21601 ccaggtgtga ccagttttat caacgacgcc atttcttttg agttcccaca gctcgttcag
    21661 cacttcttca cgactgatat caagtcgcgt agccagttct accgacgtgg cttttcccat
    21721 cgctttcagt gcgtcaaaaa cagtctccat aaatttcctc ccggtaaaaa ttacttctca
    21781 actcaaacaa acccagccgc tttccggcgt tcatattcct gtttcagcaa ctcaattggc
    21841 gttggtccca acgggcgttt tggtgccgcc agttgtcgcc gtactggcgg aacgctcagg
    21901 ccgttactaa catgctttgc ccatttcgtc agctgccgtt ctgcaagccg ttttaattcc
    21961 ccttcggtca tctggcgttc aatccccttt gaacgcatct cgaggcaaat gtgatacagc
    22021 acaggctgag accacgggta cttatcactt ccgtcgtatc gccaggactc attgcgccag
    22081 cggcggtact cctccatcac agcatccacc gtcagaccaa atggatttgc cccgctctcc
    22141 gaaatcagcg ccacaaactc agccaggtcc ggaggccatg tttcacccgc ccggcagcgg
    22201 tccatgcact ggcggcagac ctgccggatt tgctgctcag tcatcgcgcc aatctgtaca
    22261 atccagagct tcgaaggtgc ggccccgttc ttctgggtcc agcggttcga ataaatctcc
    22321 cccatgagtt cccacagctt ccaggcagtt tccgtcgttg ataaatccgt tttcacgttc
    22381 ccactgctca cgtgctgccc gaatttcctg aactgcccgt gatgcggtgc cacctggtgc
    22441 tgctgcatgg tttaccccct tgctgactgg tttagcctgc gccctgacgt gatttacgtg
    22501 acgggcgaat ttctgctccc actgaacctg cgtgaaaact ttaccctccg ctgcccagta
    22561 gtcccggaag gcggcaagtt cagctggtgt aaattccggc tccggcagag ccactcccca
    22621 cagagccgcc cgtcgtcgaa aatccggcga cggatgccag ccatcggtca tcggaaattt
    22681 cccgatgggt tcgctcaggc cttccaggta atcaggttcc gatgtttgca acggcacgcc
    22741 atttgcctca ctgaccggag cactctcgcg cacgcgcgcg ttatgtgtgg ggtttaattc
    22801 tttatctgta tctttatctg tcgtgactcg tcgtgacatg tcgtgacata tgcgtgactc
    22861 gtcgcgcgcc ccctcattct gttttcgtaa tttttccctc tcgcgctgcg ctctcttgcg
    22921 ctctgccggg gatttcgcgg tttgtgaaac gttgccattg tcctctttca gtacctggcg
    22981 tttttcccat ccactgatca aatctccatc aagtacccgc ccctgcattg cctgtaaaat
    23041 tgaatcaatt acttcttccg tcacatcaag cgcacttgct aaatcttccg tcgtgacatc
    23101 aatgtgacca cgtagtgaca cgccgtgaca tgtcgtgaca tttcgtgacg cgctcaccag
    23161 aaggtggata tacacagcca tcactgttgc gattggctgc cctgacaccc tggcaattgt
    23221 tcgccactta gggtcatttg gcatgtcatg ccataatctg agccaggcgt tagccatact
    23281 cacctctttt gataccgaat ctttttactc acaaattgcc ggaagtgatc cggtatgaat
    23341 attgtcagtc aatgcacaac cacaatattt cccgccgggc caccacgatt catctggttg
    23401 aaaccagcga tcgccactgc gacaaaatca tcagcgtctc tcaccagtcg ttcccgcgtc
    23461 tccaccagct cccgaaaata ggctgaactg tggctgcgca ttcgggccac cagcagaggt
    23521 ggcattgctt tttcgatcgc tggtaacaac gcctgaattt ttttaaccgc atcaggggtg
    23581 tctttctcca cccagcggaa tattttctga gtattgcgag ccagggcttc cggatggctg
    23641 tcgtcatata gctccgggaa cgtcattccc agctcgaaat aagcctgggt tattccagct
    23701 gctggaactt tttcgccatc aggacgcgcc caggcattca tcgccatgcg gatatgttca
    23761 tgtttgattt tcatgaatca ctcttccttt tgtcccgggt ggtatccttc tttttgtaaa
    23821 gctctgggtt caaagataat tttcccttgg agtatgcagc agcttccgca gctctccctt
    23881 tcggaactat ttcaccgggg cgcttacgcc acatgtaaat agcttcgcgg gttatcccat
    23941 aaaaatcagc gaccctctga acagagccaa aaaactgaac aagttcatca actcgcattt
    24001 tactctccta aaatctaagt atttttagat tacaggataa ttttttttag gtcaatgcaa
    24061 tctaaaataa tttatattca actcgcggga gaaaatgatg gaaagccttg gcatcaggct
    24121 taagaaactc agaaaagata agggactcac ccaagtagaa ctgggtaagc tttcaggcgt
    24181 gactggggtt actatagggt actgggagaa agatctaaac gaacccggca gcaaagctct
    24241 aagtaagtta gcccaggcat taggaactac tgagtcctat ctcctgtatg gagtatcgtc
    24301 tcctgaacta tcttttgtgc agagcaatcc aggcaccaag atcccctact tttcgtgggg
    24361 tgacgcgatt tctttcctaa tcttagaagg agagaaaaca atgggaaatg tcgataggat
    24421 caccacattc tttgatgtag tggaaggtga ttttgccgtt tcaatgcctg atgacactat
    24481 gcataacccc tcagggtcac caagtatccc ggttggtgcc actgtgatcc taagaccagg
    24541 agagagttat aaaaatggca gtatcgtcgc tgtaatagtt ccaga
//
LOCUS       DAJOWB010000066        24401 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJOWB010000066 DAJOWB010000000
VERSION     DAJOWB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.066"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            145..828
                     /locus_tag="OHO85_002850"
     CDS             145..828
                     /locus_tag="OHO85_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCQ2672197.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            872..5722
                     /gene="ehaG"
                     /locus_tag="OHO85_002851"
     CDS             872..5722
                     /gene="ehaG"
                     /locus_tag="OHO85_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HCQ2672198.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPSKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6090..7745
                     /gene="lldP"
                     /locus_tag="OHO85_002852"
     CDS             6090..7745
                     /gene="lldP"
                     /locus_tag="OHO85_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCQ2672199.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7745..8521
                     /gene="lldR"
                     /locus_tag="OHO85_002853"
     CDS             7745..8521
                     /gene="lldR"
                     /locus_tag="OHO85_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCQ2672200.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8518..9708
                     /gene="lldD"
                     /locus_tag="OHO85_002854"
     CDS             8518..9708
                     /gene="lldD"
                     /locus_tag="OHO85_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCQ2672201.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9894..10367
                     /gene="trmL"
                     /locus_tag="OHO85_002855"
     CDS             9894..10367
                     /gene="trmL"
                     /locus_tag="OHO85_002855"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCQ2672202.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10420..11241)
                     /gene="cysE"
                     /locus_tag="OHO85_002856"
     CDS             complement(10420..11241)
                     /gene="cysE"
                     /locus_tag="OHO85_002856"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCQ2672203.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11321..12340)
                     /gene="gpsA"
                     /locus_tag="OHO85_002857"
     CDS             complement(11321..12340)
                     /gene="gpsA"
                     /locus_tag="OHO85_002857"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCQ2672204.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12340..12807)
                     /gene="secB"
                     /locus_tag="OHO85_002858"
     CDS             complement(12340..12807)
                     /gene="secB"
                     /locus_tag="OHO85_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCQ2672205.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12870..13121)
                     /gene="grxC"
                     /locus_tag="OHO85_002859"
     CDS             complement(12870..13121)
                     /gene="grxC"
                     /locus_tag="OHO85_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCQ2672206.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13263..13694)
                     /locus_tag="OHO85_002860"
     CDS             complement(13263..13694)
                     /locus_tag="OHO85_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCQ2672207.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13939..15483
                     /gene="gpmM"
                     /locus_tag="OHO85_002861"
                     /gene_synonym="pgmI"
     CDS             13939..15483
                     /gene="gpmM"
                     /locus_tag="OHO85_002861"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCQ2672208.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15493..16776
                     /gene="envC"
                     /locus_tag="OHO85_002862"
     CDS             15493..16776
                     /gene="envC"
                     /locus_tag="OHO85_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCQ2672209.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16780..17739
                     /locus_tag="OHO85_002863"
     CDS             16780..17739
                     /locus_tag="OHO85_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCQ2672210.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17726..18760)
                     /gene="waaH"
                     /locus_tag="OHO85_002864"
     CDS             complement(17726..18760)
                     /gene="waaH"
                     /locus_tag="OHO85_002864"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HCQ2672211.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(18999..20024)
                     /gene="tdh"
                     /locus_tag="OHO85_002865"
     CDS             complement(18999..20024)
                     /gene="tdh"
                     /locus_tag="OHO85_002865"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCQ2672212.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(20034..21230)
                     /gene="kbl"
                     /locus_tag="OHO85_002866"
     CDS             complement(20034..21230)
                     /gene="kbl"
                     /locus_tag="OHO85_002866"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCQ2672213.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            21444..22376
                     /gene="rfaD"
                     /locus_tag="OHO85_002867"
     CDS             21444..22376
                     /gene="rfaD"
                     /locus_tag="OHO85_002867"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCQ2672214.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNKGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVMEYMAWLNRDA"
     gene            22386..23432
                     /gene="rfaF"
                     /locus_tag="OHO85_002868"
     CDS             22386..23432
                     /gene="rfaF"
                     /locus_tag="OHO85_002868"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCQ2672215.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALFGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23436..>24401
                     /gene="rfaC"
                     /locus_tag="OHO85_002869"
     CDS             23436..>24401
                     /gene="rfaC"
                     /locus_tag="OHO85_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCQ2672216.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAM"
BASE COUNT         6133 a         6322 c         6320 g         5626 t
ORIGIN      
        1 gatacatttt tatgatgaat gcttaaaaca atatttaatc gacacacatc actgtcatta
       61 atgtaaagca acatagatca ccaataatac aatggacaac tctaccggca ttgatcgctg
      121 gtcggaggaa ataaggattt ttacatgaac ttgaagaaga tttttttcag tgctgtcact
      181 gtttcagttc tctgcgcatt aacgggatgt gactacattg aagaaggcaa gccagaaagt
      241 agtttattga aacaacaaga agaacataac aataaaattg atctacttga aaaacaacaa
      301 gcacagctaa agagtcaact ggaaactatt caaaaacaac aaactggcat aataagcagt
      361 acaaagactt taactcacgt aattaaatcc gttaaagatc aacaaaacac ttttattttt
      421 accgagttta acccagcaaa aacgaagtat ttcatcctga acaatggttc ggtggcttta
      481 gcgggtcgag tgttatccat tgacgccaca gagaatggca gtgttattca tatttcactg
      541 gtcaacttat taagtacacc tatctcaaat attggtttta atgcgacatg gggtggtgaa
      601 aaacctgtcg atgccaaaga gtttgcccgg tggcaacaat tacttttcaa cacatcaatg
      661 aaatccacat tgaaattatt accaggtcaa tggcaagaca ttaatttgac cctgaagggt
      721 gtatcgccca ataacctggg atatctgaag ttagccatca acatggaaaa tattcagttc
      781 gacaatcttc cctctgctga aaatcggcag aaaagaagca aaaaataaat gcagttagta
      841 cgcttataac aaggaatagc gagcatcatt aatgaacaaa atatttaaag ttatctggaa
      901 ccctgcgaca gggaattata ctgttaccag cgaaacggca aaaagccgtg gcaagaaatc
      961 tgggcgcagt aagctgttaa tttctgcgct ggttgcgggt ggaatgttgt cgtcgtttgg
     1021 ggcattggcg aatgccggga atgacaacgg tcagggtgtt gattacggta gtggatcagc
     1081 tggcgacggc tgggttgcta taggcaaagg ggcgaaagca aatactttta tgaacaccag
     1141 tggttccagt actgctgtgg gttatgacgc tatagctgaa ggccaatata gctctgccat
     1201 cgggtcaaaa acccatgcga ttggcggtgc atcaatggcc tttggggtta gtgcaatatc
     1261 agaaggcgat agaagtatag cactgggtgc ctcttcgtat tcattgggcc aatactcaat
     1321 ggccctcggc cgttattcaa aagcattggg taaattgtct attgctatgg gggactcttc
     1381 caaagcggaa ggagcaaacg ccattgccct gggaaatgcc actaaagcta ctgagattat
     1441 gagtattgct cttggcgaca ccgccaatgc gtcaaaagcg tattcaatgg cgctgggagc
     1501 aagtagcgtc gcatctgaag aaaacgcaat tgccctgggg cgtagcagtg tagctagcgg
     1561 tactgacagc ctcgcatttg gcagacaatc acttgccagc gcagcgaacg ctattgcgat
     1621 aggtgctgag accgaagccg ctgaaaatgc aactgctatt ggcaataatg cgaaggcaaa
     1681 agggactaat agcatggcaa tggggttcgg aagccttgcc gataaagtca atactatcgc
     1741 attaggaaat ggcagccagg ctctggcaga taatgcaatc gccataggcc agggcaacaa
     1801 agctgatggc gtggatgcca tcgctctggg taatggtagc cagtcgagag gcttaaacac
     1861 cattgcctta ggcacagcca gtaatgcaac tggtgataag agtcttgcgc ttggtagtaa
     1921 tagcagtgcc aacggtatta actctgtcgc actgggcgca gattccattg cggatttaga
     1981 caataccgtc tctgtcggca atagttcatt aaaacgcaag atcgttaatg tgaaaaatgg
     2041 cgcgatcaag tctgacagtt acgatgccat taatggttca cagctttatg ccattagcga
     2101 ctcggtagca aaaaggcttg gaggaggggc tgcagtagat gttgatgacg gtactgttac
     2161 agcaccaacc tacaatttaa aaaatggtag caaaaataac gtaggggctg cgctcgctgt
     2221 acttgatgaa aacaccctgc aatgggacca aaccaaaggc aaatacagcg ctgctcatgg
     2281 tactagtagc ccaactgcca gcgtaatcac cgatgttgcg gatggcacga tttcagcctc
     2341 cagtaaggat gcggttaacg gttcccaact gaaagctacc aatgacgatg tcgaagccaa
     2401 caccgccaat atcgctacta ataccagcaa cattgccacg aatacggcaa atattgccac
     2461 caataccacc aatatcacca acctgacgga ttccgttggt gaccttcagg ctgatgccct
     2521 gctctggaac gaaactaaaa aggcattcag tgcagctcac ggccaggata ccaccagcaa
     2581 aatcaccaac gttaaagatg ccgacctgac ggctgacagc actgatgctg ttaacggctc
     2641 tcagctgaaa accaccaacg atgctgtggc gacgaatacc accaatatcg ccaataacac
     2701 ttccaatatt gccactaaca ccaccaacat ctctaacctg actgagacgg tgactaatct
     2761 tggtgaggat gcgctgaaat gggataagga caatggtgta ttcacggcag ctcatggcac
     2821 cgagaccacc agcaaaatca ccaacgttaa agatggcgac ctgacgactg gcagcaccga
     2881 tgccgttaac ggctctcagc tgaaaaccac caacgatgcc gtggcgacga ataccaccaa
     2941 tatcgccact aacaccacca acatctctaa tctgactgag acggtgacta atcttggtga
     3001 ggatgcgctg aaatgggata aggacaatgg tgtcttcact gcagctcatg gcaacaatac
     3061 cgccagcaaa atcaccaata tcctggacgg cacagtcact gcaaccagtt ccgatgccat
     3121 taacggtagc cagctttatg acttaagcag caatatcgcc acctacttcg gcggcaatgc
     3181 ttctgtgaat actgacggtg tgtttaccgg tccaacctac aaaatcggtg aaacaaatta
     3241 ttataacgtc ggcgatgcac tggctgcgat taactcctca tttagcacgt ctctcggcga
     3301 tgctctgctt tgggatgcca ccgcaggtaa attcagtgcc aaacacggta ctaatggtga
     3361 cgcaagcgtg atcactgatg tcgcagatgg tgaaatttca gactccagtt ctgacgcagt
     3421 aaacggctca caactccacg gcgtgagcag ttatgttgtt gatgcgctgg ggggtggtgc
     3481 cgaagtcaat gcagacggca ccatcactgc gccgacgtac accattgcta atgctgatta
     3541 cgataatgtc ggtgatgccc tgaatgctat cgataccact cttgacgacg ctctgctctg
     3601 ggatgcggac gccggtgaaa atggtgcatt tagcgccgct cacggaaaag ataaaactgc
     3661 cagtgtaatc actaacgtcg ctaacggtgc aatctctgct gccagcagcg acgcgattaa
     3721 cggctcacaa ctctatacca ccaataagta catcgctgat gcgctgggtg gtgacgcaga
     3781 agtcaacgcc gacggcacca tcaccgcacc gacttacacc attgcgaacg ccgagtacaa
     3841 caacgtcggt gacgccctgg atgcgcttga tgataacgcc ctgctgtggg atgagactgc
     3901 caatggcggt gctggagcct acaatgccag ccatgacggt aaagccagca tcatcactaa
     3961 tgtcgctaat ggcagtatta gtgaggacag taccgatgca gtgaacggtt ctcagttgaa
     4021 tgcgacgaat atgatgattg agcagaacac ccaaattatc aatcagctcg ctggtaacac
     4081 cgacgcaacc tatatccaag aaaacggtgc gggtattaac tatgtgcgta ctaacgacga
     4141 cggcttagcg ttcaacgacg ccagcgcaca gggtgttggc gctacagcta taggttataa
     4201 ctctgtcgcc aaaggcgata gcagcgtagc tattggtcag ggcagctaca gcgacgttga
     4261 tacgggtatc gccctgggta gcagctctgt ttccagccga gtgattgcca aaggctcccg
     4321 tgacaccagc ataacggaaa atggcgttgt tattggttac gacaccacgg atggcgaact
     4381 gctcggtgca ttgtctatcg gtgatgacgg taaatatcgt caaatcatca acgtagccga
     4441 tggttccgaa gcccatgacg ccgttacggt tcgtcaattg cagaatgcga ttggtgctgt
     4501 agcaacgaca ccgtccaaat acttccatgc taattcaacg gaagaagatt cactggcagt
     4561 gggaactgac tcgctggcaa tgggtgcgaa aaccatcgtg aatggcgata aaggtattgg
     4621 tatcggttat ggtgcctacg tggacgcgaa tgcacttaac ggcattgcca ttggtagcaa
     4681 tgcgcaagtc attcatgtca acagtattgc gataggtaat ggttctacga ccactcgtgg
     4741 cgctcaaacc aattataccg cctacaacat ggacgcaccg cagaactctg tcggtgaatt
     4801 ctcagttggt agtgcggatg gtcaacgtca gatcactaac gtcgcagcag gttcggctga
     4861 taccgatgcg gtcaacgtgg gtcagttgaa agtaacggat gcgcaggttt cccagaatac
     4921 ccagagcatt actaacctgg ataatcgggt aacgaatctt gattcacgcg tcaccaatat
     4981 cgaaaacggt attggcgata tcgtcaccac cggtagcacc aagtacttca agaccaatac
     5041 cgatggtgta gatgccagcg cgcagggtaa agatagcgtt gctattggtt ctggttccat
     5101 tgctgccgct gacaacagcg tcgcactggg taccggttcc gttgcagagg aagaaaatac
     5161 aatctctgta ggttcttcta ctaaccaacg ccgtatcacc aacgtagctg ccggtgttaa
     5221 tgccaccgat gcggttaacg tgtcgcagct gaaatcttct gaagcaggcg gcgtgcgcta
     5281 cgacaccaaa gctgatggtt ctatcgacta tagcaatatc accctcggtg gcggcaacgg
     5341 cggtacgact cgtatcagca acgtctccgc tggcgtcaac aacaacgacg cggtgaatta
     5401 cgcgcagttg aagcaaagcg tgcaggaaac gaagcaatac accgatcagc gaatggttga
     5461 gatggataac aaactgtcta aaactgaaag caagttgagc ggtggtatcg cttctgcaat
     5521 ggcaatgacc ggtctgccgc aggcttacac tccaggtgcc agcatggcct ctattggtgg
     5581 cggtacttac aacggtgaat cggcagttgc tttaggtgta tcgatggtga gcgccaatgg
     5641 tcgttgggtc tacaaattac aaggtagtac caatagccag ggtgaatact ccgccgcact
     5701 cggtgccggt attcagtggt aatgatccat taagttagtg tgactaaggg caaacgccaa
     5761 attgcctgat gcgttacgcg catcaggcaa gataaggcgc tccgggtcaa caatctaagg
     5821 gcaattctct gatgaggatt gcccttttct ttaccagaca tctcccccca caagaattgg
     5881 ccctaccaat tcttcgctta tctgacctct ggttcacaat tccccaatta aaactcacat
     5941 caatattgcc aatacataac atttagttaa ccattcattg tcattatccc tacacaacac
     6001 aattggcagt gccactttta cacaacgtgt gacaaggaga tgagcaacag actcattaca
     6061 cgatgtgcgt ggactccagg agacctgcaa tgaatctctg gcaacaaaac tacgatcccg
     6121 ccgggaatat ctggctttcc agtctgatag catcgcttcc catcctgttt ttcttctttg
     6181 cgctgattaa gctcaaactg aaaggatacg tcgccgcctc atggacggta gcaatcgccc
     6241 ttgccgtggc tttgctgttc tataaaatgc cggtcgctaa cgcgctggcc tcggtggttt
     6301 atggcttctt ctatggtttg tggcccatcg cgtggatcat tattgcagca gtgttcgtct
     6361 ataagatctc ggtgaaaacc gggcagtttg acatcattcg ctcgtctatt ctttcgataa
     6421 cccctgacca gcgtctgcaa atgctgatcg tcggtttctg tttcggcgct ttccttgaag
     6481 gagccgcagg ctttggcgca ccggtagcaa ttaccgccgc attgctggtc ggcctgggtt
     6541 ttaaaccgct gtacgccgcc ggactgtgcc tgattgttaa caccgcgcca gtggcatttg
     6601 gcgcgatggg cattccaatc ctggttgccg gacaagtaac aggcatcgac agctttgaga
     6661 ttggccagat ggtggggcgg cagctaccgt ttatgaccat tatcgtgctg ttctggatca
     6721 tggcgattat ggacggctgg cgcggtatca aggagacgtg gcctgcggtc gtggttgcgg
     6781 gcggctcgtt tgccatcgct cagtacctta gctctaactt cattgggccg gagctgccgg
     6841 acattatctc ttcgctggta tcactgctct gcctgacgct gttcctcaaa cgctggcagc
     6901 cagtgcgcgt attccgtttt ggtgatttgg gggcgtcaca ggttgatatg acgctggccc
     6961 acaccggtta cactgcgggt caggtgttac gtgcctggac accgttcctg ttcctgacag
     7021 ctaccgtaac actgtggagt atcccgccgt ttaaagccct gttcgcatcg ggtggtgcgc
     7081 tgtatgagtg ggtgatcaac attccggtgc cgtacctcga taaactggtt gcccgtatgc
     7141 caccagtggt cagcgaggct acagcctatg ccgccgtgtt taagtttgac tggttctctg
     7201 ccaccggcac cgccattctg tttgctgcac tgctctcgat tgtctggctg aagatgaaac
     7261 cgtctgacgc tatcagcacc ttcggcagca cgctgaaaga actggctcta cccatctact
     7321 ccatcggcat ggtgctggcg ttcgccttta tctcgaacta ttccggactg tcatcaacac
     7381 tggcactggc actggcgcac accggtcatg cattcacctt cttctcgccg ttcctcggct
     7441 ggctgggggt attcctgacc gggtcggata cctcatctaa cgccctgttc gccgcgctgc
     7501 aagccaccgc agcacaacaa attggcgtct ctgatctgtt gctggttgcc gccaatacca
     7561 ccggtggcgt caccggtaag atgatctccc cgcaatctat cgctatcgcc tgtgcggcgg
     7621 taggcctggt gggcaaagag tctgatttgt tccgctttac tgtcaaacac agcctgatct
     7681 tcacctgtat ggtgggcgtg atcaccacgc ttcaggctta tgtcttaacg tggatgattc
     7741 cttaatgatt gttttaccca gacgcctgtc agacgaggtt gccgatcgtg tgcgggcgct
     7801 gattgatgaa aaaaacctgg aagcgggcat gaagttgccc gctgagcgcc aactggcgat
     7861 gcaactcggc gtatcacgta attcactgcg cgaggcgctg gcaaaactgg tgagcgaagg
     7921 cgtgctgctc agtcgacgcg gcggcgggac gtttattcgc tggcgtcatg acacatggtc
     7981 ggagcaaaac atcgtccagc cgctaaaaac gctgatggcc gatgatccgg attacagttt
     8041 cgatattctg gaagcccgct acgccattga agccagcacc gcatggcatg cggcaatgcg
     8101 cgccacacct ggcgacaaag aaaagattca gctttgcttt gaagcaacgc taagtgaaga
     8161 cccggatatc gcctcacaag cggacgttcg ttttcatctg gcgattgccg aagcctcaca
     8221 taacatcgtg ctgctgcaaa ccatgcgcgg tttcttcgat gtcctgcaat cctcagtgaa
     8281 gcatagccgt cagcggatgt atctggtgcc accggttttt tcacaactga ccgaacaaca
     8341 tcaggctgtc attgacgcca tttttgctgg tgatgccgac ggggcgcgta aagcaatgat
     8401 ggcgcacctt agttttgttc acaccaccat gaaacgattc gatgaagatc aggctcgcca
     8461 cgcacggatt acccgcctgc ccggtgagca taatgagcat tcgagggaga aaaacgcatg
     8521 attatttccg cagccagcga ttatcgcgcc gcagcgcaac gcattctgcc gccgttcctg
     8581 ttccactata tggatggggg tgcatattct gaatacacgc tgcgccgcaa cgtggaagat
     8641 ttgtcagaag tggcgctgcg ccagcgtatt ctgaaaaaca tgtccgactt aagcctggaa
     8701 acgacgctgt ttaatgagaa attgtcgatg ccggtggcgc taggtccggt aggtttgtgt
     8761 ggcatgtatg cgcgacgcgg cgaagttcag gcagccaaag cggcggatgc gcatggcatt
     8821 ccgtttactc tctcgacggt ttccgtttgc ccgattgaag aagtcgcgcc agccatcaag
     8881 cgcccaatgt ggttccagct ttatgtactg cgcgatcgcg gctttatgcg taacgcgctg
     8941 gagcgagcaa aagcagcggg ttgttcgacg ctggttttca ccgtggatat gccgacaccg
     9001 ggagcgcgtt atcgtgatgc gcattctgga atgagcggcc cgaacgcggc aatgcgccgc
     9061 tatttgcagg cggtgacgca tccgcaatgg gcgtgggatg tgggcctgaa cggtcgtccg
     9121 catgatttag gaaatatttc ggcttacctc ggcaaaccga ccggactgga agattacatc
     9181 ggctggctgg ggaataactt cgatccgtcc atctcatgga aagaccttga gtggatccgc
     9241 gatttctggg atggcccgat ggtgatcaaa gggatcctcg atccggaaga tgcgcgcgat
     9301 gcagtacgtt ttggtgctga tgggattgtg gtttctaacc acggtggccg ccagctggac
     9361 ggtgtgctct cttctgcccg tgcactgcct gctattgccg atgcggtgaa aggtgatatc
     9421 gccattctgg cggatagcgg aattcgtaac gggcttgatg tcgtgcgtat gattgcgctc
     9481 ggtgccgaca ccgtactgct gggtcgtgct ttcctgtatg cgctggcaac agcgggccag
     9541 gcgggtgtag ctaacctgct aaatctgatc gagaaagaga tgaaagtggc aatgacgctg
     9601 actggcgcga aatcgatcag tgaaattacg caagattcgc tggtgcaggg gctgggtaaa
     9661 gagttgccta cggcactggc tccgatggcg aaagggaatg cggcatagtc gtttgagccc
     9721 cctcacccta accctctccc tcagggagtg gggaccgttc ggcgctgtat gtactccctc
     9781 actctgaaac gacaccgcac tctttttttc tccctcgccc ctccggggtg aggggaaaag
     9841 gccgcactgc tcaccaattt tctgctatcc tgcccccgca ctaagggggc agtatgctaa
     9901 acatcgtact ttacgaacca gaaattccgc caaatactgg caacatcatc cgtctttgcg
     9961 ctaataccgg ctttcgtctg catatcatcg aaccgatggg atttgcctgg gacgataagc
    10021 gcctgcgccg cgcggggctg gactatcacg agtttaccgc cgttacgcgt catcatgact
    10081 atcgcgcgtt cctcgaagca gaaaatcccc agcgcctgtt tgccctcacc acgaaaggta
    10141 cgcctgctca cagcgccgta agctatcagg atggcgatta tctgatgttc ggcccggaaa
    10201 cacgcggcct gccagcgagc attcttgatg ccctgcccgc tgaacaaaaa attcgcattc
    10261 cgatggtgcc ggacagccgc agcatgaatc tgtccaatgc ggtgtcggta gtggtgtatg
    10321 aagcctggcg gcagctggga tatccgggag cgttgttgag agattagtta ctgtatgccg
    10381 gatagatgat tacatcgcat ccggcacgat cacaggacat tagatcccat ccccatactc
    10441 aaatgtatgg ttaataccgt tgaaatgctg gtccatatcc attgatggct tatcgctgtc
    10501 tggtttaccg acaatacgag ccggaacgcc agcggcggtt gtatgcggcg gcaccggttg
    10561 cagcaccacg gaacctgcgc caatcttcgc gccgcgacca acttcaatat tgccgaggat
    10621 tttcgcgccc gcgccaatca tcacaccttc acgaattttc gggtgacggt caccaccaga
    10681 tttacccgta ccgccaagcg tcacagattg cagaatcgat acgtcgtttt caattaccgc
    10741 cgtttcaccc acaacgatgc ccgtggcgtg gtcgagcata atgccacggc caatttttgc
    10801 tgccgggtga atatcgacct ggaacgtcac agaaacctgg ttttgcagaa agattgccaa
    10861 tgcgcgacgc ccctgattcc acaaccagtg accgatgcga taggcctgca aggcatgaaa
    10921 acccttcagg tataacaacg gggttgagta tttatcgact gccgggtcgc gggtacgcac
    10981 cgcctgaata tcacaggccg cagaggcgat catttccggg tcagcggcgt aggcttcttc
    11041 caccacttca cggatagcaa tagcaggcat aattggcgat gacagcttgt tcgccagcat
    11101 gtagctcagt gcactaccaa ggttttcgtg cttgagtagc gtcgcgtggt aaaaactggc
    11161 cagcattggc tcacagtccg ccagcgttct ggcttcggct ttaatattgt tccagacaat
    11221 ttccagttct tcacacgaca ttgcttactc cacacgatga gataatgacc ggcccgttct
    11281 gcgcgggccg ggtcatagcg gtaacaaagg ttccctgggg ttagtggctg ctgcgctcgt
    11341 ccttgcgtgc acgacctaat aacgtcaatg ctgcctcgcg cgcgtttttt ccgcaatata
    11401 atacttgata aatttcctcg gttattggca tttcaacgcc gaagcgatgc gccagttcgc
    11461 ggacttcttt cgtattgcgg tagccttcca ccacctgacc aatcttctcc tgcgcgcttt
    11521 gtacatccat gccctgaccg agcatcatgc caaaacggcg gttacgcgac tggttgtcgg
    11581 tacaggtaag caccagatcg ccaagccccg ccatgcccat aaaggtggca ggatcggcac
    11641 ccagcgccgc accaagacgc gacatttcag ccagcccacg ggtgatcagc gccgtacgcg
    11701 cattcgcacc aaaaccgata ccgtcggaca tccccgcgcc aatggcaatg acgtttttca
    11761 ccgcaccgcc aagctgcaca ccgatgaaat ccgggttgct gtaaacgcgg aaacttttgc
    11821 cacagtgcag caattgttgg agatcatcgg caaaggtctg gtcggtagag gccaacgaaa
    11881 tagccgtcgg taaacctgcc gccagttctt tcgcaaacgt tgggccagag ataaccgcca
    11941 gcggaatttg atcgcctaag gcctcacgcg ccacgtcctg taacagacgt ccggtttccg
    12001 cttccagccc tttggtcgcc cacaccagac gcgcatcagg acgcatcagc ggtttaatct
    12061 ggcgcagcac ttcaccaaag acatggctgg gtacgacgac gagaatatta cggctggctg
    12121 ccagcgcagt ggcgagatcg ctttcaagat ggagcgtatc gggaaaaggc acatcgggga
    12181 gaaacgcggc gttacagcgg tcgcgttcaa gcgttgcgat atgttcaggg tcatggcccc
    12241 agaggacaac ctcgtggcca tttcttgcca gggtaatggc aagagcggtg ccgtacgagc
    12301 cggcaccgat cacagtcatt gaagcattac gttggttcat caggcatcct gatgttcttc
    12361 agtaccttcg ccagcctgct gctgcaaata gttcatgaac agcgcatcga agttaaccgg
    12421 cgcaaggttc agttgcggga atgtaccgcg ggataccatg ctggtgatgc actcacgagc
    12481 atacgggaac agaatgttcg ggcagtatgc gcccaggcaa tgtgccatct gggtgccttc
    12541 gatacccgcg atggagaaaa taccgccctg ctgaacttca cacaggaacg cggtttcttc
    12601 gcccagggag gccgttacgg taacacgcag taccacttcg taaacgtcat ctgccagttg
    12661 ggaagatgcc gtatccagat caagtttaac ttctggctgc caatctttct ggaaaacgtg
    12721 cggcgcgttc ggcgcttcga aagaaatatc cttggtataa atacgttgga tctggaaagt
    12781 catttcggtg ttgttttgtt ctgacatgtg tagaaaaccc ttaagtgttg tccttaaata
    12841 ctgcgcaatg taatgccaac gttcacacgt tatttcagca ggggatccag tccaccacgt
    12901 gcatccaatg catacaagtc atcacagccg ccaatgtgct gtgcgtcaat aaaaatctgg
    12961 ggaaccgtgg tgcgaccgct gcgtttgatc atctcttcac gcttggcggc attgccatcg
    13021 atcggcaact cctgaaaact cacgcccttg ctgctcagca gtgcttttgc acgatggcaa
    13081 tacgggcagg tttctttggt atagatttca acattggcca taatttagct cctgtttttt
    13141 taccctgaaa tttcatgttg cagggcggct gcaaaatgga gtgtccccag aagcttataa
    13201 caataagtcg ctgggggcct aattgcagct aacggcctgc atcatgaaag acgacaggta
    13261 aattatttgc cgcgcaccaa aggcaagttt tcgccagccc agccagcgac gccttctttc
    13321 agcacgaata cttgcgcaaa accagctttc gtcagtgcgt ttgcaggctc ctggcactgc
    13381 atgccagaac cgtctaccac gataaccggt ttgtctttgt gcttctcaag ctcaccaaca
    13441 ttgttggctt tgatttcgct cggcaacaga ttgatagaac ctgcgatatg gcctttacgg
    13501 aagtcatcac gctgacgtaa atccacaacc acagcgtctt ctttgttgat cagacgcgta
    13561 gcttcaccac gagtaatcac cttcactttc gaggtcaggc tcttaaacgt agtcacaaga
    13621 accgccacca gtaacgcgat ccaggcgata ctcagtatgg gatgacggcc aacaaattgc
    13681 ataatttctt gcatgggggg taacaactcc cgacgtagtg attaaaaaaa ccaggaaagg
    13741 agtatacctg cgcggtgcgg caaatacagc cagcgcgtta actggaatgc aattttgcgg
    13801 ggcacgacga aaaaaaagcg caaatctggc atcggcttgc cgcgagcggt cgtatatttt
    13861 gatcttcaga ggctatttta tcgattcagc tgtagtaaaa ttacgcaaat tttgactctt
    13921 gagtatgagg ttgtcgcaat gtcggtttct aaaaaaccta tggtactggt gattctggat
    13981 ggctatggct atcgtgaaga acagcaggat aacgccattt ttagtgctaa aaccccggta
    14041 atggatgcac tgtgggccaa tcgtccgcat accctgatcg acgcttccgg tctggaagtc
    14101 ggtctgcctg accgtcagat gggtaactcc gaagtaggcc acgttaacct gggtgccggc
    14161 cgcatcgtgt atcaggacct gactcgtctg gacgttgaaa tcaaagatcg cgctttcttt
    14221 gctaatccag tgctgactgg cgcggtagat aaagcgaaaa acgcaggtaa agcagtacac
    14281 attatgggtc tgctctccgc aggcggcgta cacagccacg aagatcacat catggcgatg
    14341 gtagaactgg cagctgaacg cggcgcagaa aaaatctacc tgcacgcatt ccttgacggt
    14401 cgcgacaccc cgccgcgcag tgctgaatcc tcgctgaaaa aatttgaaga gaaatttgct
    14461 gcgctgggta aaggccgcgt agcgtccatc attggtcgtt actacgcgat ggaccgcgat
    14521 aaccgttggg atcgcgttga aaaagcctat gacctgctga ctctggcgca gggtgagttc
    14581 caggccgata ctgccgttgc tggtttgcag gcagcttatg ctcgcgacga aaacgacgag
    14641 ttcgtgaaag cgaccgttat tcgtgcagaa ggtcagccag atgcggcaat ggaagacggc
    14701 gatgcgctga ttttcatgaa cttccgtgct gaccgcgcgc gcgaaatcac tcgtgcattc
    14761 gtaaacgcag atttcgacgg cttcgcgcgt aagaaagtgg ttaacgtcga tttcgtgatg
    14821 ctgaccgaat acgccgctga catcaaaact gcggttgctt acccacccgc ttccctggtt
    14881 aacaccttcg gcgagtggat ggcgaaaaac gacaaaactc agttgcgtat ttccgaaacc
    14941 gaaaaatatg cccacgttac tttcttcttc aacggtggcg tagaagagtc gttcaaaggc
    15001 gaagatcgca ttctgatcaa ctcaccgaaa gtggctacct acgatctgca accggaaatg
    15061 agctccgcag agctgaccga aaaactggtt gcggccatca agagcggcaa atacgacacc
    15121 attatctgta actatccgaa cggcgacatg gtaggtcaca ccggggtgat ggaagcggcg
    15181 gttaaagcgg ttgaagcact ggatcactgc gtggaagaag tcgcgaaagc ggttgagtcc
    15241 gtgggtggac aactgctgat caccgctgac cacggtaacg ctgaacagat gcgcgatccg
    15301 gcaacgggtc aggcacacac ggcacacacc aacctgccag ttccgctgat ttacgttggt
    15361 gataagaacg tgaaagcggt tgcaggcggc aaactttctg acatcgcgcc gaccatgttg
    15421 tcgctgatgg gtatggaaat cccgcaagag atgactggta agccgctgtt catcgtggaa
    15481 taatccctcc ccatgagggg aaaggcgatt aataccatga cacgggccgt gaaaccgcgc
    15541 aggtttgcaa tcaggcccat catctacgcc agcgttctta gcgctggcgt attgttgtgc
    15601 gccttttccg cccacgcgga tgagcgtgac caactcaaat ctattcaggc cgatatcgcc
    15661 gcgaaagagc gcgcggtacg ccaaaagcaa caacaacgcg caagcctgct cgcacaattg
    15721 aaaaagcagg aagaagcgat ctctgaagcc acccgtaagc tgcgcgaaac gcaaaacacg
    15781 ctcaatcaac tcaataaaca gattgatgag atgaacgcgt cgattgccaa actggagcag
    15841 caaaaagccg cccaggagcg cagcctcgcc gcacaactgg atgccgcatt ccgtcagggc
    15901 gagcataccg gtattcagct aattctcagc ggtgaagaaa gccagcgtgg acagcgttta
    15961 caggcttatt tcggctatct caaccaggcg cgacaagaaa ccattgccca gttgaagcaa
    16021 acgcgtgaag aagtcgccat gcagcgtgct gaactggaag agaaacagag cgagcaacaa
    16081 acgctgttat atgaacagcg cgcccaacag gcgaaactga ctcaggctct gaacgagcgt
    16141 aaaaagacgc tggcggggct ggaatcttcc atccagcaag gtcagcagca gctaagtgaa
    16201 ctgcgtgcca acgaatcccg tctgcgtaac agcattgccc gtgcggaagc cgcggcgaaa
    16261 gcgcgtgctg aacgagaagc gcgtgaggcc caggcggttc gcgaccgcca gaaagaagcg
    16321 acgcgcaaag gcaccaccta taaaccgacc gaaagcgaaa aatcgctgat gtcccgaact
    16381 ggtggcctgg gtgcgccgcg tggtcaggca ttctggccgg ttcgcgggcc gacgctgcat
    16441 cgctatggtg aacagctaca gggcgaacta cgctggaaag gaatggttat cggtgcctct
    16501 gaaggtactg aagttaaagc gattgccgac ggtcgggtga ttctggctga ctggctgcaa
    16561 ggttacggtc tggtggtggt ggttgagcac ggtaaaggcg acatgagtct ttacggctat
    16621 aatcagagcg cactggtgag cgttggttcg caggttcgcg cgggccagcc aattgcactg
    16681 gtgggcagca gtggcggtca gggtcggcct tcactctatt tcgaaattcg ccgccagggt
    16741 caggcggtca atccacagcc gtggttggga agataagttt tgtttccatt tcgtcgtaac
    16801 gttcttgcat ttgccgctct gttggcgctc tcctcccccg tacttgctgg caaacttgcc
    16861 atcgtcattg atgattttgg gtatcgcccg cacaacgaaa accaggtgct ggcgatgccc
    16921 tccgctatct ccgtcgctgt attacccgat tcaccgcacg ccagagagat ggcgaccaaa
    16981 gcgcataaca gcgggcacga agtattgatt catctcccga tggcaccact gagtaaacag
    17041 ccgctggaga aaaatacgct gcgcccggag atgagcagcg acgaaattga gcgcattatt
    17101 cgtagtgcgg tcaataacgt gccctatgcc gtggggatca acaaccacat gggtagcaag
    17161 atgacctcta acctgtttgg tatgcagaaa gtgatgcagg cactggagcg ttacaatctt
    17221 tacttcctcg acagcgtaac catcggtaat acccaggcga tgcgcgccgc gcaaggtact
    17281 ggcgtgaagg tgatcaaacg gaaggtgttc ctcgacgatt cgcagaacga agcggacatc
    17341 cgtgtgcaat ttaatcgcgc aattgatctg gcgcgtcgca acggttcaac cattgccatt
    17401 gggcatcctc acccttcaac ggtacgcgtg ctacaacaga tggtttataa cctgccgcca
    17461 gacattacgc tggtgaaagc cagcagcttg ctgaatgaac cgcaggttga tacttctaca
    17521 ccgccgaaaa acgctgtgcc tgatgcaccg cgtaatccgt tccgtggcgt gaagctgtgc
    17581 aaaccgaaga aaccgataga acctgtttat gctaatcgct tctttgaagt attaagcgaa
    17641 agcatcagcc agagcacgct gatcgtttac ttccagcatc agtggcaagg ctggggcaaa
    17701 cagcccgaag cggcgaagtt taacgctagc gcaaattaaa ggcgctacgc gtaatgcgat
    17761 ggtgcgaccg cgttttgtcg cgccattgcc ataagcggaa agaccacagt aacgcctggt
    17821 aaccgacctt cacgctgcgc acattggtaa tcaggcgctt atacataccg gaagtaaata
    17881 tctcggcaat catccgttgg cgggtaagaa tatccggctc tttgcgcacc gcatggcaaa
    17941 cacgcaatgc ttcgtaagtt atttgctgat gaaattccgg ataaatcgta attttgtcgg
    18001 cataatttcg atttaatttc tccagcaggc gggtaatctt aatatagtga cgttgataat
    18061 taaggttttt attcccttgt ctatgcaacc gactcactga cgtattatgc agataatatt
    18121 tatataatga ttgctcggta tatcgcgcac gcagcgcgtt aaacatgaat tctgttgtcc
    18181 agacaatatc ctgatgatgt aatccggcaa taaatttaat gttattttta acaataacat
    18241 cacggcgata aacccccatc cagacaacgt gagtccaacg gcgcgaagaa agccccatcc
    18301 gcagccagtc cgggccggtt aatacgccgg ttgagcgaag gcgatcgctg gggatggatt
    18361 gccaggtttc tcccgtttca cgaaaacacc agtcagcgtt gcactgcgcc acgtcgaggt
    18421 catcctctaa cgccatggtc atcagcgttt cgtacatggt gggatagacc tcatcgtcag
    18481 catcgacaaa agcgacatat ttgcccgtcg ccacctcgat cccacgatta cgcgctaccg
    18541 atgcgccagc attcgcctga tgcaacaaac gaacgtgcgg atagttttct gcgtaatgct
    18601 ttgctatttc aacagaatta tccgttgaac catcgttaat aataatgatt tccagagcag
    18661 tccaggtttg cgtaattaaa gattccatac aagtgcggaa atcatcgccc gcattatata
    18721 acggaataat aacactaagt ttattggtgc tgttcatcat aaattaccga cgacctctga
    18781 agttatcctg acgttttaca ggctttgtat taagaaacta ttaagcctgt gagatattat
    18841 ttttacaggt gagagagtca cttcagggta gagagataat gagagcagct atataagaga
    18901 tcagcgcaat tacgatatct ttattacact gacaggattt tacatattta acggattatc
    18961 ctcaggcaaa agggctggaa taccagccct tgttcgtgtt aatcccaact cagaattact
    19021 ttcccggact ggcccgaacg catagcgtca aagcccttct ggaaatcatc gatagagaaa
    19081 cgatgggtaa tgatcggcga aagatcgagg ccagactgaa tcagcgccgc catcttgtac
    19141 caggtttcaa acatctcacg accgtaaata cctttaatga acaagccttt aaagatcact
    19201 ttggtccagt cgatagacat atcagacggc ggaataccca gcatcgcaat acggccgccg
    19261 tggttcatgg tatcaagcat ggtacgaaac gctggcggcg caccggacat ttccagaccg
    19321 acatcaaaac cttcggtcat gcctaactcc gccatcacgt cattgagatt ttctttggcg
    19381 acgttaaccg cacgggtgat acccatttta cgcgccagct caaggcggta ttcgttaaca
    19441 tcagtgatca ccacattgcg tgcaccaacg tgtttcgcca ccgccgctgc cataatacca
    19501 atcgggcctg caccagaaac cagcacatcc tcgccaacca gatcgaacga cagcgcggta
    19561 tgcacggcat taccgaaggg atcaaaaatg gaagccaggt cgtcggagat attgtcggga
    19621 attttgaagg cgttgaacgc cggaatcacc agatattcgg cgaagcagcc cgggcggtta
    19681 acgccgacgc cgatcgtgtt gcggcacaga tgggtacgac cgccacgaca gttgcgacag
    19741 tgaccgcagg tgatatggcc ttcgccagaa acgcgatcgc caatcttgaa gcccttcact
    19801 tcctgaccaa tacctaccac ttcaccgaca tattcatggc ccacgaccat cggcaccggg
    19861 atggtttttt gcgaccactc atcccagtta tagatgtgaa cgtcagtccc gcagatggct
    19921 gttttacgga ttttaatcag cagatcgtta tgcccgagtt ccggtacagg aacgtcggtc
    19981 atccagatgc cctcttccgc tttcagtttg gataacgctt tcatctcaca tcctcaggcg
    20041 ataacgccca gttgtttacc aatacgcgtg aatgcttcta ctgcacgcgt aatttgctca
    20101 ggggtatgcg ccgcagacat ctgggtacga atacgcgcct gacctttcgg aacgaccgga
    20161 tagaagaaac cggtgacgta aatgccctct ttttgcagct cacgggcaaa tttctgcgcc
    20221 actaccgcat caccaagcat gaccggaata atggcgtgat cggctcccgc cagggtaaag
    20281 cccgccgccg acatttgctc acggaactga cgcgcgttcg cccacagacg gtcacgcaac
    20341 tcgctacccg cttcaaccat ctccagcact ttgatggaag cggcgacaat cgctggtgcc
    20401 agcgagttgg agaacaggta cggacgagaa cgctggcgca gccactcaac cacttctttg
    20461 cgcgccgcgg tataaccacc agaagccccg cccagcgctt taccgagcgt gccggtgatg
    20521 atgtcgacac gccccatcac atcgcagtat tcatgggaac cacgaccatt ttcaccgaca
    20581 aaaccgaccg cgtgggagtc gtctaccatc accagggcat cgtatttatc tgccagatcg
    20641 caaacgccct tcagattggc aatcacgccg tccatcgaga acacaccatc ggtggcaatc
    20701 agcacatgac gcgcaccggc ttcacgcgct tctttcagac gagcttcaag ctcctgcata
    20761 tcgttgttgg catagcgata gcgtttagct ttacacagac gcacaccatc aataatagac
    20821 gcgtggttca gtgcgtcgga gataatggcg tcttccgcgc ccagcagcgt ttcaaacagg
    20881 ccaccgttag catcaaagca ggaagagtag agaatcgcat cttccatccc caggaatgcc
    20941 gccagttttt gttcaagctc tttatggctg tcctgagtgc cgcaaataaa acgcaccgaa
    21001 gccatgccga aaccgtgaga atccattccc gcctttgctg ccgcaatcag atccggatga
    21061 ttcgccagcc cgagatagtt gttggcacaa aagttaatga cgtggcttcc atcagccacg
    21121 gtgatatctg cttgctgcgc agacgtaata atgcgctctt ctttaaacaa cccttccgcc
    21181 cgtgcggttt ccagatcgtt ggttaactgc tgataaaatt ctccacgcat tgcgattctc
    21241 cagacagggc aaattccagc acatattacc caaacttata ggtcgcgacg agataacgcg
    21301 ttaacacttc tgcaaaattc aggataaata acgtccatca atctcacttt tggcgaatgg
    21361 ctgaagccaa cgccgtgtag tgatatgata gaagtattcg tgtctgagat tgtctctgac
    21421 tccataattc gaaggttaca gttatgatca tcgttaccgg cggcgcgggc tttatcggca
    21481 gcaacatcgt taaggccctg aatgataaag gcatcaccga tattctggtg gtggacaacc
    21541 tgaaagacgg caccaagttt gtgaacctgg tggatctgga tatcgcggac tatatggata
    21601 aggaagactt cctgatccag attatggctg gcgaagagtt cggcgatgtc gaagcgattt
    21661 tccacgaagg cgcatgctct tccaccaccg agtgggacgg caagtatatg atggataaca
    21721 actatcaata ctccaaggag ctgctgcact actgcctgga gcgcgaaatc ccgttcctgt
    21781 acgcttcttc cgcagccacc tacggcggac gcacctccga ctttattgaa tcccgcgagt
    21841 acgaaaaacc gttgaacgtc tacggttact caaaattcct gtttgatgaa tatgttcgcc
    21901 aaatcctgcc tgaagcgaac tcgcagattg ttggcttccg ctatttcaac gtttatggac
    21961 cgcgtgaagg ccataaaggc agcatggcga gcgtcgcttt ccatctcaac actcagctta
    22021 acaaaggtga atcaccgaag ctgtttgaag gtagcgagaa cttcaaacgt gacttcgtct
    22081 atgtgggcga cgtggcagat gtaaacctgt ggttcctgga aaatggcgtt tccggtatct
    22141 tcaacctcgg tactggtcgt gcggaatcct tccaggccgt agcagatgct actctggctt
    22201 atcacaagaa aggccagatc gaatacattc cgttcccgga taagctgaaa ggccgctacc
    22261 aggcgttcac tcaggcagat ctgacaaatc tgcgcgcggc gggttacgac aaaccgttca
    22321 aaaccgttgc tgaaggtgta atggaataca tggcctggct gaatcgcgac gcataagagc
    22381 tctgcatgaa aatactggtg atcggcccgt cttgggttgg cgacatgatg atgtcgcaaa
    22441 gtctctatcg cacgctccag gcgcgctatc cccaggcgat aatcgatgtg atggcaccgg
    22501 catggtgccg tccattatta tcgcggatgc cggaagttaa cgaagctatc cctatgcctc
    22561 tcggtcacgg agcgctggaa atcggcgaac gccgcaaact gggtcatagc ctgcgtgaaa
    22621 agcgctacga ccgcgcctac gtcttaccaa actccttcaa atctgcatta gtgcctttct
    22681 tcgcgggtat tcctcatcgc actggctggc gcggcgagat gcgctacggt ttactcaacg
    22741 atgtacgcgt gctcgataaa gaagcctggc cgctaatggt ggaacgctat gtcgcgctgg
    22801 cctatgacaa aggcattatg cgtaccgcac aagatctgcc gcagccattg ttatggccgc
    22861 agttgcaggt gagcgaaggt gaaaaatcat atacctgtaa tcaattttcg ctttcatcag
    22921 aacgtccgat gattggcttt tgcccgggtg cggagtttgg tccggcaaaa cgctggccac
    22981 actaccacta tgcggagctg gcaaagcagc tgattgatga aggttatcag gtggttctgt
    23041 ttggctctgc gaaagatcat gaagcgggca atgagattct tgccgctttg aataccgagc
    23101 agcaggcatg gtgtcggaac ctagcggggg aaacacagct tgatcaagcg gttatcctga
    23161 ttgcagcctg taaagccatt gtcactaacg attctggcct aatgcacgtt gcggcggcgc
    23221 tcaatcgtcc gctggttgcc ctgtttggtc cgagtagccc ggacttcaca ccgccgctat
    23281 cccataaagc gcgcgtgatc cgtctgatta ccggctatca caaagtgcgt aaaggtgacg
    23341 ctgcggaggg ttatcaccag agcttgatcg acattactcc ccagcgcgta ctggaagaac
    23401 tcaacgcgct attgttacaa gaggaagcct gacggatgcg ggttttgatc gttaaaacat
    23461 cgtcgatggg cgatgttctc catacgttgc ccgcactcac tgatgcccag caggcaatcc
    23521 cagggattaa gtttgactgg gtggtggaag aagggttcgc acagattcct tcctggcacg
    23581 ctgccgttga gcgagttatt cctgtggcaa tacgtcgctg gcgtaaagcc tggttctcgg
    23641 cccccataaa agctgaacgc aaagcgtttc gtgaagcgct acaagcagag aactatgacg
    23701 cagttatcga tgctcagggg ctggtaaaaa gcgcggcgct ggtgacgcgt ctggcgcatg
    23761 gcgtaaagca tggattggac tggcaaaccg ctcgcgaacc gttagccagc ctgttttaca
    23821 atcgtaagca tcatattgca aaacagcagc acgccgtaga acgcacccgc gaactgtttg
    23881 ccaaaagtct gggctatagc aaaccgcaaa cccagggcga ttatgctatt gcacagcatt
    23941 tcctgacgaa cctgcctaca gatgctggcg aatatgccgt atttcttcat gcgacgaccc
    24001 gtgatgataa acactggccg gaagaacact ggcgagaatt gattggttta ctggctgatt
    24061 caggaatacg gattaaactt ccgtggggcg cgccgcatga ggaagaacgg gcgaaacgac
    24121 tggcagaagg atttgcttat gttgaagtat tgccgaagat gagtctggaa ggcgttgccc
    24181 gcgtgctggc cggggctaaa tttgtagtgt cagtggatac ggggttaagc catttaacag
    24241 cggcactgga tagacccaat atcacggttt atggacctac cgatccggga ttaattggtg
    24301 ggtatgggaa gaatcagatg gtttgtaggg ctccggggaa tgagttgtct caattgacag
    24361 caaatgctgt taagcggttc attgaagaaa acgctgccat g
//
LOCUS       DAJOWB010000067        24304 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJOWB010000067 DAJOWB010000000
VERSION     DAJOWB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.067"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(113..994)
                     /gene="prmA"
                     /locus_tag="OHO85_002870"
     CDS             complement(113..994)
                     /gene="prmA"
                     /locus_tag="OHO85_002870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCQ2672217.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(1006..2457)
                     /gene="panF"
                     /locus_tag="OHO85_002871"
     CDS             complement(1006..2457)
                     /gene="panF"
                     /locus_tag="OHO85_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCQ2672218.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLVAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2447..2689)
                     /locus_tag="OHO85_002872"
     CDS             complement(2447..2689)
                     /locus_tag="OHO85_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCQ2672219.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVSAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2798..4147)
                     /gene="accC"
                     /locus_tag="OHO85_002873"
     CDS             complement(2798..4147)
                     /gene="accC"
                     /locus_tag="OHO85_002873"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCQ2672220.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4158..4628)
                     /gene="accB"
                     /locus_tag="OHO85_002874"
                     /gene_synonym="fabE"
     CDS             complement(4158..4628)
                     /gene="accB"
                     /locus_tag="OHO85_002874"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCQ2672221.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5606..6580)
                     /locus_tag="OHO85_002875"
     CDS             complement(5606..6580)
                     /locus_tag="OHO85_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCQ2672222.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            6732..8672
                     /gene="csrD"
                     /locus_tag="OHO85_002876"
     CDS             6732..8672
                     /gene="csrD"
                     /locus_tag="OHO85_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCQ2672223.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            8977..10020
                     /gene="mreB"
                     /locus_tag="OHO85_002877"
     CDS             8977..10020
                     /gene="mreB"
                     /locus_tag="OHO85_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCQ2672224.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10086..11189
                     /gene="mreC"
                     /locus_tag="OHO85_002878"
     CDS             10086..11189
                     /gene="mreC"
                     /locus_tag="OHO85_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCQ2672225.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            11189..11677
                     /gene="mreD"
                     /locus_tag="OHO85_002879"
     CDS             11189..11677
                     /gene="mreD"
                     /locus_tag="OHO85_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCQ2672226.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11686..12279
                     /gene="yhdE"
                     /locus_tag="OHO85_002880"
     CDS             11686..12279
                     /gene="yhdE"
                     /locus_tag="OHO85_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCQ2672227.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12269..13738
                     /gene="rng"
                     /locus_tag="OHO85_002881"
     CDS             12269..13738
                     /gene="rng"
                     /locus_tag="OHO85_002881"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCQ2672228.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13806..17606
                     /gene="yhdP"
                     /locus_tag="OHO85_002882"
     CDS             13806..17606
                     /gene="yhdP"
                     /locus_tag="OHO85_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCQ2672229.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGVRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17762..19207
                     /gene="tldD"
                     /locus_tag="OHO85_002883"
     CDS             17762..19207
                     /gene="tldD"
                     /locus_tag="OHO85_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCQ2672230.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19335..20264)
                     /gene="aaeR"
                     /locus_tag="OHO85_002884"
     CDS             complement(19335..20264)
                     /gene="aaeR"
                     /locus_tag="OHO85_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCQ2672231.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            20447..20650
                     /locus_tag="OHO85_002885"
     CDS             20447..20650
                     /locus_tag="OHO85_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCQ2672232.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            20658..21590
                     /gene="aaeA"
                     /locus_tag="OHO85_002886"
     CDS             20658..21590
                     /gene="aaeA"
                     /locus_tag="OHO85_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCQ2672233.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            21596..23563
                     /gene="aaeB"
                     /locus_tag="OHO85_002887"
     CDS             21596..23563
                     /gene="aaeB"
                     /locus_tag="OHO85_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCQ2672234.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            23655..23927
                     /locus_tag="OHO85_002888"
     CDS             23655..23927
                     /locus_tag="OHO85_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCQ2672235.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(23983..24246)
                     /gene="yhcN"
                     /locus_tag="OHO85_002889"
     CDS             complement(23983..24246)
                     /gene="yhcN"
                     /locus_tag="OHO85_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCQ2672236.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5746 a         6132 c         6608 g         5818 t
ORIGIN      
        1 tttatgaatg aaaattttgg cacgcaaatt tgcgtaaata atcggcaaat ttacgtgcct
       61 tttgtgagct tgctcgcact tcgccccgcg tcaccctacg gcgatgcgaa ggttaattct
      121 tacgaccggt aatgcggcac cactcttctt tttccacgac cgggtccagt gcgaagctgt
      181 cgacataagc ttcacaaacg ctctctgcct ggcttgccag aataccggaa aggcccagca
      241 aaccgcctga aaccggcagg acgctgatta acggtgccag ttcacgtaat gggcctgcaa
      301 ggatgttagc gaccaccacg tcggctttca tttcttctgg ctgatctttc ggtaagtaga
      361 gttccagacg gtcagaaacg ccattacgtt cggcgttgtc gcggctggcc tgaatcgcct
      421 gcggatcgat atcaatacca atggcttttg ctgcacccag tttcagcgcc gcgatcgcca
      481 gaatgccgga accacagcca aagtcgatga ctgttttacc ggttaaatcg aggctgtcga
      541 gccattgcag gcacagagag gtggttggat gggtacccgt accaaacgcc agccctggat
      601 ctaacatcac gttgacggcg ttttcgtccg gcacatcacg ccagctaggg cagatccaca
      661 gtcgttcacc aaagcgcatc gggtggaaat tatccatcca ttcgcgctcc cagtctttat
      721 cttctagttg ttcgatttta tgcgcgaagc ctgcgccgag cagcggatgg ttttccagaa
      781 tcgccaccac gtcgttcata tcggtttcag cgtcgaacag accaatcaca tcggtgtcgc
      841 cccacaggcg cgtttcgccc ggcagcggct caaatactgg cgtatcgtgg gtatcctgaa
      901 aagtgataga aacggcaccc gcttccatca gcgcatcgct aagatcttcc gcgttcgcgc
      961 cggtggtgtt cagtttcagt tggatccaag gcatggcaaa actctttatt tatcagtagt
     1021 caaaacggta gcttgcggga cggatgtacc gaaacggttt ccgaccagga aagccaccaa
     1081 acttagtagt aacgagggca cgatagggtg gaagcccagg tactgaatat tcagcgtcgc
     1141 gagtacggca tacagcacgc cgccaacgat catcgcactt agcgcgcctt tggcgttggc
     1201 gcgttcccag taaagaccca gcaccagcgg ccacaggaaa acggcttcca gcccaccgaa
     1261 ggccagcaaa ttcagccaga tgatcatttc tggcggcttc caggcggcaa gcagcagcaa
     1321 cgcgccgaga actaacgtaa ttaccgccga catccgcttc agacgcgtct cgttttgcat
     1381 ttgatccgga cggatattca gatagagatc tttaatgatc gtagcggaac tttgtagcaa
     1441 ttgggcgtta attgtcgaca tgatcgcagc cataggtgca gccagaaaga tcccggcagc
     1501 aaacggtggc agcactttta ccattaacgt cgggatcacc agatccggta cggtgagatc
     1561 ggggatcact gcacgaccta acgctccggc caggtgcata ccgaacatca ggattgcgac
     1621 tacaatcgta ccgatgatga tcccccggtg tactgctttg ctgtctttat aagagataca
     1681 gcgcaccgca gtatgcggca ggccaatcac gccaaagcac accagtaccc agaacgacgt
     1741 cataaaggca ggcgacagaa tatcgtcagc gccttgtggc gtaaccagtt gcggatcgat
     1801 ggtttgcaag gtctgtactg cgttgcttaa gccgccagcg gcatgtacca cgccaataag
     1861 cagcacaacg gtgccaatca gcatcacaag cccttgcatg gtgtcgttca gcacgctggc
     1921 gcgaaagccg ccaaaggcgg tatacaacgc aatgctgata ccgaaaatca gcagcccggt
     1981 ttcataagga atacccgccg cggtttccag caggcgcgca ccgccgataa actgcacggt
     2041 cattgcccca acgaacgcaa ccagcaaact caaactcgcc agccacacca gaagacgact
     2101 ctggtagcgg gcaaacagca tatcgttcag cgtcactgca ttgtagcggc gcgcaagaat
     2161 cgcgaacttc ttgccgagaa taccgagcga aagccagact gcaggaagct gaatcatcgc
     2221 cagcaatacc caacccaggc cgtatttata agcagctcca ggcccgccga taaacgaact
     2281 ggcactgata taggtcgcgg tgagcgtcat cgccagcaca ataccgccca tagagcggct
     2341 gccgaggaaa tactcattaa ggaaggtgcc ggtgctccgt ttacgcatcg cataaaccga
     2401 gataccgaac accaccacca gataggcgac cagcggtaga attacttcaa gctgcatcgt
     2461 catcctccag tgggatatcg cgatagataa atttcaccat cgcccagcac agtccaataa
     2521 acagtagcgg cgtcaggatg caggccatct caaaccagcg cggaaagccg gtaaaaccgg
     2581 gggcaacgcc aggtaagtaa gcggatacta accaaactgc cagatacaaa agggtcagcc
     2641 ccagcgccca gcgcgcctct ttatgggcct gaacaaaacg agtgtccatt ttttgtccct
     2701 gatgggtgaa gaaagcgagg attgtacctt atgggggttg tcgatcccca gtaataaaaa
     2761 aggccggaaa atccggcctt ttgacgcttt tgcagtctta tttttcctga agaccgagtt
     2821 ttttctccag atagtggatg ttagtgccac catgctggaa gttctcgtca ttcatgatgc
     2881 ggatctgcag atcaacattg gttttgatac cgtcgatgat cagctcctgc agcgcattct
     2941 tcatgcgggc aatcgccacg tcacggtttt caccgtagca aatcagctta ccgatcattg
     3001 agtcatagta cggcggtacg gtgtagcccg cgtagatatg agactcccaa cgtacgccaa
     3061 aaccgccagg tgcgtggaaa cgggtgattt tgcctgggct tggcaggaag gtgttcggat
     3121 cttcggcgtt gatacgacat tccaccgcat ggccgcgaac gtgaacttct tcttgcttga
     3181 tcgacagcgg ttgaccggca gcgatacgca gctgttcttt gatcaggtca acgccggtga
     3241 tcatttcggt aaccgggtgt tctacctgaa tacgggtgtt catttcgatg aaatagaact
     3301 cgccgttttc gaacaggaac tcgaaagtac ctgcaccgcg atagccgata tcaacacacg
     3361 ctttagcgca acgttcgccg atgtagcgac gcagttccgg ggtaatgccc ggtgctggcg
     3421 cttcttcgac cactttctgg tggcggcgct gcatggagca gtcacgttcc gccagataga
     3481 tagcgttgcc ctgaccgtca gccagtacct gaatctcgac gtggcgagga ttttccaggt
     3541 atttctccat gtaaaccata tcgttgctga aagcagcttt cgcttccgca cgggtcatgg
     3601 agatggattg tgccagttca gcgtcgccgc gcactacgcg cataccgcga ccgccgccgc
     3661 cgccggaggc tttgataatc accggataac caatgcgttt agcaatggcg cggtttttat
     3721 ccatatcgtc gcccagcggg ccgtcagaac caggtacgca aggaacgccc gcttttttca
     3781 tcgccgcgat tgcggatact ttgtcgccca tcaggcgaat ggtttctgct ttcgggccga
     3841 tgaagataaa gccggagcgt tcaacctgct cggcaaagtt ggcgttctcg gagaggaagc
     3901 cgtaacccgg atggattgct actgcgccgg tgatttcagc ggcgctgatg attgccggga
     3961 tgttcagata actttttact gacggagcag ggccaataca gaccgtttca tctgccagta
     4021 atacgtgttt tagatcgcga tccgcgctgg agtgcacagc gacagtcttg atgcccagtt
     4081 ctttacaggc acgaagaata cgcaatgcaa tctcgccgcg gttggcaata acaattttat
     4141 ccagcatgtt cgcctcgtta ctcgatgacg accagcggct cgtcaaattc taccggttgt
     4201 ccactttcga ccagaattgc tttcacggta ccggatttgt ccgcttcgat ctggttcatc
     4261 attttcatgg cttcaacgat gcacagggta tcgcccacgt tgactttctg acccacttcg
     4321 atgaacgctt ttgcgtccgg gcttggggtg cggtagaaag taccaaccat cggggaacgt
     4381 acgatgtgac cactgatttc cgctgctgct ggcgcttcca tggaaggaac ggtcgccgga
     4441 gcggctgcgt tagattgagc tggctgctgc atcattggtg cagcgtaagc ttgttgcatc
     4501 acagggaaac ttgcggcagg agctgcacgg ctaatgcgta ctgactcttc gccttcagaa
     4561 atttccagtt cggagatgcc tgattcttca accagctcga tcagtttttt aatcttacga
     4621 atatccatga gtgggttccg tactctttgt ttagtgtgat tgtgacaggc gtttcaccgc
     4681 cgtctgtaaa gcgtatgccc cgacagctgt atgcatagcg ataaattcca gcaggccggg
     4741 acgccggtct attttgcccc agagtgtcaa tgttagactt gacggacatt gtgcagcgat
     4801 ctgctgctat cctccggcaa aaaacaaaat ataccttcgt tgcgccaatg tcaccttttc
     4861 cgcagcgcaa aaactgcgtc agggaggacg aggtcgcaca ttataacgat ttcgtagcaa
     4921 ttggcagcta aatactggtc ttatcaggga agataatcaa cagctaacat gtaaataacc
     4981 ttcaacaccg tgtaatttgc aacaagccgc acaattcacg aaattagcgc catcatcgac
     5041 ggaacttctt ataacgtaag gtgaaaaact gtaaagccag ccctgtgaag atgaggggct
     5101 gcaatgagat aatcttcaca gaccacaaat aatgtaggga tgccaggatc gcgacaagat
     5161 agacgacatt acgcagcggt tgctgaggat tacccaattt tcactgcatc gtctggatag
     5221 acgtgaatgc taaagcacgc aaaatcaacc gactgacaat gctcagcatt aaataggttt
     5281 gagtggttaa tttgtagtca aataaaatca gattattcac cccatggtac cagaaataag
     5341 taaggggcta agcgtagcgt cgcccaggca aagcaccata aacctaacag gcgacggagg
     5401 cgtatcaata atagctgttt agtggagtat caatgcgcat caatttgtgg tcagtcgaaa
     5461 tcatgacttt acagtttgga tgatgttgta taactttctg accaaggtgt tcgacgaaag
     5521 ctgtccggct aaccgctttt cccgttaacc gattgaataa tgatttcaaa atgagttccc
     5581 tctcttatta ttccctgcta aatggttagt taaccttcac cagcgtgcga ccctggatct
     5641 ggttattaat gatggcctcg gcaaagttcg gtgcctctga cagagatatc tcttttgccg
     5701 cctgggtata gaatgattcc ggtaaatcgg cgaccagtcg ctgccaggct tgtgcgcggc
     5761 gttctggtgg cgtcattact gaatccaccc cttgcaaacg gacattacgc agaataaatg
     5821 gcatgaccgt ggttggcaga gtaaaaccac ccgccagacc acaggcagcc acgcagccgc
     5881 cgtaattcat ttgcgccagc acttttgcca gcactttgtc gccaacagtg tcaattgccc
     5941 cagcccagac ttgtttttcc agaggacggg attcggcaaa ctcatcacga gggagaatac
     6001 gactggcacc taaacttttc agatattcat gggtactttc gcgaccggaa acggcaacga
     6061 cctgataacc caacttatgc agcagcgcca cggcggtact gccgacgcca ccactggcac
     6121 ccgtcacgac aatctccccg tcctgcgggc gaacaccggc atcttccagc gccatcacac
     6181 acagcatggc ggtaaaaccg gcagtaccga taatcattgc tttacgcgcg tccagccctt
     6241 gcggcatggc aaccagccag tcacctttca ctcgcgcctg ctccgccagc ccaccccagt
     6301 ggttttcacc aacgccccag ccagtgagta acacctcctg accggcatga aaacgcggat
     6361 cttcgctggt gcgtacagtt ccggcaaaat cgatcccagg aatcatcgga aaattacgga
     6421 tgatttttcc cttaccggta atcgccagcg catctttata gttcaggctc gaccagtgaa
     6481 catcaaccgt gacatcgccc tccggcaggc gactttcgtc aagtgtctgt accgatgcga
     6541 gagttttgcc gtcctgctgt tctaaaagta acgcctgcat aagtggtcct catgtgcatg
     6601 atggattgga aaataatttc tgaagactat actcgctaat ggaaaagcaa agccgatgaa
     6661 gcgcaataaa ttgcgagata aatctgattt gctagtatgc ccgcttcctc actatcggag
     6721 ttaacacaag gatgagatta acgacgaaat tttcggcctt tgttacgctg ctcaccgggt
     6781 taacaatttt tgtgactttg ctgggctgtt cgctaagttt ctacaacgca attcagtata
     6841 agtttagtca tcgcgttcag gcggtggcga cggcgattga tacccacctt gtgtcgaatg
     6901 acttcagcac attaaggcca caaattaccg aattaatgat gtcggcagat atcgttcgtg
     6961 tagacctgct ccatggtgat aaacaggttt ataccctggc cagaaatggt agttatcgtc
     7021 cggttggctc cagcgatctg tttcgcgaac tgagtgttcc gttgataaag catccgggga
     7081 tgtcgctgcg tctggtttat caggatccga tgggcaacta tttccattct ttaatgacta
     7141 ccgcgccgct cacgggggcg attggcttta tcattgttat gctcttcctg gcggtacgct
     7201 ggttacaacg gcaacttgcc gggcaagaat tgctggaaac ccgggctact cgtatcttaa
     7261 acggtgagcg tggctctaat gtgttgggaa ccatctatga atggccgccc agaaccagca
     7321 gtgcgctaga tacgctgctt cgtgaaattc agaacgcacg cgaacaacac agccgtcttg
     7381 atacgctgat ccgctcttat gccgcccagg acgtgaaaac cggcctcaat aaccgactct
     7441 ttttcgataa tcagttagca acgttactgg aagatcagga gaaagtaggt acccacggga
     7501 tcgtgatgat gattcgtctg ccggatttca atatgttgag cgatacttgg gggcacagcc
     7561 aggttgaaga acagttcttc actctgacga atctgctgtc gacatttatg atgcgctacc
     7621 ctggcgcact gctggcgcgt taccaccgca gtgattttgc tgcgctgtta ccgcaccgga
     7681 cgttaaaaga ggcagagagc atcgccggtc agttaatcaa agccgtcgat accttgccga
     7741 acaataaaat gctcgatcgc gacgatatga tccacattgg tatctgcgcc tggcgtagtg
     7801 gtcaggatac cgagcaggta atggaacatg cagagtctgc cacgcgtaat gcgggattgc
     7861 agggcggcaa tagctgggct atttacgatg actcgttgcc tgaaaaagga cgcggtaatg
     7921 ttcgctggcg tacgcttatc gagcaaatgc tcagtcgcgg cggcccgcgc ctttatcaaa
     7981 aaccggcggt tactcgcgaa ggtcaggttc atcatcgcga actcatgtgc cgcatcttcg
     8041 atggtaatga agaggttagc tcggcggagt atatgccgat ggtcttgcag tttggcttat
     8101 cggaagagta tgaccgtctg caaatcagcc gtcttattcc actattgcgt tactggccag
     8161 aggaaaatct ggcgattcag gttaccgttg agtcgctgat tcgcccgcgt tttcagcgtt
     8221 ggctgcgcga tacgttaatg caatgtgaaa aatcacaacg aaaacgcata attattgaac
     8281 ttgcagaggc cgatgtaggt caacatatca gtcgtttaca acctgttatt cgtttagtaa
     8341 atgctttagg ggtacgggta gccgtcaacc aggctggttt gacgctggta agcaccagtt
     8401 ggatcaaaga acttaatgtt gagttactca agctccatcc ggggctggtc agaaacattg
     8461 agaagcgaac ggagaaccag ctgctggttc aaagcctggt ggaagcctgc tccgggacca
     8521 gcacccaggt ttacgccacc ggtgtgcgtt cgcgaagcga gtggcagacc ctgattcagc
     8581 gcggtgttac aggcgggcaa ggggattttt tcgcgtcctc acagccactt gatactaacg
     8641 tgaaaaaata ttcacaaaga tactcggttt aacctgccgt ttaatccgtt ttcacgtaga
     8701 ataacgcgcg ctgcgtctca tgggagtgtg cttgtctgct cgccagattg ttgcagcaca
     8761 tatgcagatg aatgacctta cgcggttgca aacaggcgag gaatgctgct gatgcattaa
     8821 gcctttctgg actcaggcag agatttgtaa caaaggaaac gaactgcact aattttcacc
     8881 gtagcagatg atttttgcgc cttgtcgctg ctgcgtgtgg ttggtaaagt aagcggattt
     8941 tcttttccgc cccagctttc aggattatcc cttagtatgt tgaaaaaatt tcgtggcatg
     9001 ttttccaatg acttgtccat tgacctgggt actgcgaata ccctcattta tgtaaaagga
     9061 caaggcatcg tattgaatga gccttccgtg gtggccattc gtcaggatcg tgccggttca
     9121 ccgaaaagcg tagctgcagt aggtcatgac gcgaagcaga tgctgggccg tacgccgggc
     9181 aatattgctg ccattcgccc aatgaaagac ggcgttatcg ccgacttctt cgtgactgaa
     9241 aaaatgctcc agcacttcat caaacaagtg cacagcaaca gctttatgcg tccaagcccg
     9301 cgcgttctgg tttgtgtacc ggttggcgcg acccaggttg aacgccgcgc aattcgtgaa
     9361 tccgcgcagg gcgctggtgc ccgtgaagtc ttcctgattg aagaaccgat ggctgccgca
     9421 attggtgctg gcctgccggt ttctgaagcg accggttcta tggtggttga tatcggtggt
     9481 ggtaccactg aagttgctgt tatctccttg aacggcgtgg tttactcctc ttccgtgcgc
     9541 attggtggtg atcgtttcga cgaagctatc atcaactatg tgcgtcgtaa ttacggttct
     9601 ctgatcggtg aagccaccgc agaacgtatc aagcacgaaa tcggttcggc ttacccgggc
     9661 gatgaagtcc gtgaaatcga agttcgtggc cgtaacctgg cagaaggtgt tccacgcggt
     9721 tttaccctga actccaacga aatcctcgaa gcactgcagg aaccgctgac cggtattgtg
     9781 agcgcggtaa tggttgcact ggaacagtgc ccgccggaac tggcttccga catctccgag
     9841 cgcggcatgg tgctcaccgg tggtggcgca ctgctgcgta accttgaccg tttgttaatg
     9901 gaagaaaccg gcattccagt cgttgttgct gaagacccgc tgacctgtgt ggcgcgcggt
     9961 ggcggcaaag cgctggaaat gatcgacatg cacggcggcg acctgttcag cgaagagtaa
    10021 tcggatgcag gcaggggaaa tgtctgttta ccctgcctgg tctgatacga gaatacgcat
    10081 aacttatgaa gccaattttt agccgtggcc cgtcgctaca gattcgcctt attctggcgg
    10141 tgctggtggc gctcggcatt attattgccg acagccgcct ggggacgttc agtcaaatcc
    10201 gtacttatat ggataccgcc gtcagtcctt tctactttgt ttccaatgct cctcgtgaat
    10261 tgctggatgg cgtatcgcag acgctggcct cgcgtgacca actagaactt gaaaaccggg
    10321 cgttacgtca ggaactgttg ctgaaaaaca gtgaactgct gatgcttgga caatacaaac
    10381 aggagaacgc gcgtctgcgc gagctgctgg gttccccgct gcgtcaggat gagcagaaaa
    10441 tggtgactca ggttatctcc acggttaacg atccttatag cgatcaagtt gttatcgata
    10501 aaggtagcgt taatggcgtt tatgaaggcc agccggtcat cagcgacaaa ggtgttgttg
    10561 gtcaggtggt ggccgtcgct aaactgacca gtcgcgtgct gctgatttgt gatgcgaccc
    10621 acgcgctgcc aatccaggtg ctgcgcaacg atatccgcgt aattgcagcc ggtaacggtt
    10681 gtacggatga tttgcagctt gagcatctgc cagcgaatac ggatattcgt gttggtgatg
    10741 tgctggtgac ctccggtctg ggcggtcgtt tcccggaagg ctatccggtc gcggttgtct
    10801 cttccgtaaa actcgatacc cagcgcgctt atactgtgat tcaggcgcgt ccgactgcag
    10861 gactgcaacg tttgcgttat ctgctgctgc tgtggggcgc ggatcgtaac ggcgctaacc
    10921 cgatgacgcc ggaagaggtg catcgtgttg ctaatgaacg tctgatgcag atgatgccgc
    10981 aggtattgcc ttcgccagac gcgatggggc caaagttacc tgaaccggca acggggatcg
    11041 ctcagccgac tccgcagcaa ccgacgacag gaaatgcagc tactgcgcct gctgcgccca
    11101 cacagcctgc tgctaatcgc tctccacaaa gggctacgcc gccgcaaagt ggtgctcaac
    11161 cgcctgcgcg tgcgccggga gggcaatagt ggcgagctat cgtagccagg gacgttgggt
    11221 aatctggctc tctttcctca ttgcgctgtt gctgcaaatc atgccctggc cggataacct
    11281 gattgttttc cggccaaact gggtgttact catcttgttg tattggatcc tggccttgcc
    11341 tcatcgcgta aatgtgggca caggttttgt gatgggtgcc atactggatc tgatcagcgg
    11401 ctcgacgctt ggcgtacgcg tattggcgat gagcatcatt gcttacctgg tggcgctgaa
    11461 ataccagctt ttccgcaacc tcgcattatg gcagcaggcg ctggtcgtca tgttgctttc
    11521 gctggtggtg gatattattg ttttctgggc agagttttta gtgattaacg tctctttcag
    11581 accggaagtg ttctggagta gtgtagtcaa tggggtgctc tggccgtgga ttttcttgct
    11641 gatgcgtaaa gtccgtcagc agtttgcagt gcaataaagg tttctatgac ttctctgtat
    11701 ttagcttccg gttctccgcg tcgtcaggag ttacttgcgc aacttggcgt gacctttgaa
    11761 cgtattgtta cgggcattga ggagcagcgt cagccgcagg agagcgcgca gcagtatgtt
    11821 gtgcgtctgg cgcgcgagaa agcacaggcg ggtgtcgcgc aaacggcgca ggatctcccg
    11881 gtgctgggtg cggatactat cgttatcctg aatggagaag tgctggagaa accgcgcgac
    11941 gcagagcatg cggcgcagat gttgcgcaaa ttatcgggtc agacccatca ggtgatgacg
    12001 gcagtggcgt tggccgacag ccagcacatt ctcgattgcc tggtggtcac cgatgtgact
    12061 ttcagaacgt taacagacga agacatcgcg ggctatgtcg ccagcggtga accgttagat
    12121 aaagcaggtg catacggtat tcaggggctg ggtggctgtt ttgtcaggaa gataaatggc
    12181 agctatcacg ccgtagtcgg cttaccgctg gttgaaacgt atgaattatt aagtaatttt
    12241 aacgcactgc gtgagaaaag ggataaacat gacggctgaa ttgttagtaa acgtaacgcc
    12301 ttcggaaacg cgagtggcgt atattgatgg cggtattctg caggaaattc atattgaacg
    12361 tgaggcgcga cgcggaatag taggcaatat ctacaagggt cgtgtaagtc gtgtacttcc
    12421 gggtatgcag gcggcttttg tagatattgg gctggataaa gccgcgtttc ttcatgcatc
    12481 cgatatcatg ccgcacaccg aatgtgtggc gggtgaagaa caaaagcaat tcacggtgcg
    12541 cgacatctcg gaactggttc gtcaggggca agatctgatg gtgcaggtgg tgaaagatcc
    12601 gcttggcact aaaggtgcgc gcctgaccac cgatatcacg ctgccttctc gctatctggt
    12661 gtttatgcca ggggcttctc acgttggggt ttcccaacgt attgaaagcg aatcagaacg
    12721 tgaacgcctg aaaaaagtgg tcgcagagta ttgcgacgag cagggcgggt ttatcatccg
    12781 taccgcagcg gaaggggttg gcgaggctga actggcctcc gatgccgctt atctgaaacg
    12841 cgtctggacc aaagttatgg agcgtaaaaa acgcccgcag acccgttatc agctgtacgg
    12901 cgaactggcg ctggcgcagc gtgttctgcg tgatttcgcc gacgccgaac tggaccgcat
    12961 tcgcgttgac tcacgcctga cttacgaagc attgctggag tttacctcgg agtacattcc
    13021 cgagatgacc agcaagctgg agcattacac cggacgccag ccgattttcg atctctttga
    13081 tgtcgaaaac gaaatccagc gagcgctgga acgcaaagta gaactgaaat ccggtggtta
    13141 tctgattatc gaccagaccg aagcgatgac caccgtggac atcaataccg gagcgtttgt
    13201 cggtcatcgc aatcttgacg acaccatttt caataccaat attgaagcga cgcaggctat
    13261 cgctcgccag ttacggttgc gcaatctggg cgggattatc attattgatt tcatcgatat
    13321 gaataatgaa gatcaccgcc gccgagtgct gcactcgctg gagcaggcgt tgagcaaaga
    13381 ccgggtgaaa accagcgtta atggtttttc ggcgctgggg ctggtggaga tgacgcgtaa
    13441 acgcacccgc gaaagcattg agcacgtact gtgtaacgaa tgcccaacct gccacggtcg
    13501 cggaacggtg aaaaccgtgg aaacggtatg ctatgaaatc atgcgcgaga ttgttcgtgt
    13561 ccaccatgct tacgactccg accgtttcct ggtctatgct tctccggcag tagctgaagc
    13621 cttgaaaggc gaagagtcac actcgctggc ggaagtggaa attttcgttg gcaaacaggt
    13681 taaagtacaa attgaaccgc tctataacca ggagcagttt gacgtcgtaa tgatgtaaac
    13741 agatgctggg ccgccatccg gcaaagggtt tttgagtcac atttttagca gacaaggagt
    13801 gacgggtgag gcgattgccg gggattttac tgcttactgg agccgcgctc gttgtgatcg
    13861 ctgccctgct ggttagcggc ctgcgtattg ctttaccgca tcttgacgcc tggcgtccgg
    13921 aaatcctcaa caaaatagaa tccgcgactg gcatgccggt agaagccagt cagctctcag
    13981 ccagctggca gaattttggc ccgacgcttg aagcacacga catccgtgca gaactaaaag
    14041 atggcggcga attttcggtt aaacgcgtta ctctggcgct ggatgtctgg cagagcttgt
    14101 tacatatgcg ctggcagttt cgcgacctca ctttctggca gctgcgcttt cgcaccaaca
    14161 ctcctatcac cagcggtggt ggtaatgata gcctggaagc cagtcacatc agcgatctgt
    14221 ttcttcgtca atttgaccat ttcgatctcc gcgacagtga agtcagtttt ctgacgccat
    14281 ccggtcagcg cgccgagctg gcgatcccac aactcacctg gctgaacgat ccacgtcgac
    14341 accgtgcgga aggcctggta agcctctcca gccttaccgg acagcacggc gtgatgcagg
    14401 tgcgtatgga tttgcgcgat gatgaggggt tgttaagcaa tggtcgcgtc tggctacagg
    14461 cggatgacat cgacctgaag ccgtggctcg gtaaatggat gcaggacaat attgctctgg
    14521 aaacggcaca gttctccctt gaaggctgga tgacgatcga caaaggcgat gtaaccggcg
    14581 gtgacgtctg gctgaaacag ggcggtgcca gctggttggg cgagaagcaa acgcatacgc
    14641 tgtcggtgga taatctgacc gcgcatatta cgcgtgaaaa tccgggctgg cagttctcta
    14701 ttcccgatac acggatcacg atggacggca aaccctggcc gagcggagca ttgacgctgg
    14761 cctggatacc ggaacaggac gttggcggca aagacaataa acgcagtgac gaactccgga
    14821 ttcgcgccag taatctggag ctggcaggcc tggagggcgt acgcccgctg gccgcgaaac
    14881 tttcacctgc actgggtgat gtttggcgct ccacacaacc gagcggcaag attaacactc
    14941 tggcgctgga tatcccgctt caggcggcag acaagacccg ttttcaggca tcgtggagcg
    15001 atctggcctg gaagcaatgg aaattattac cgggtgcgga acacttctcc gggacgcttt
    15061 ccggcagcgt tgaaaatggt ttgcttaccg cgtcgatgaa gcaggcaaag atgccttacg
    15121 aaacggtatt ccgtgcgcca ctggaaatcg ccgacggcca ggcaactata agctggctga
    15181 acaatgacaa aggtttccag ctggatgggc gtaatattga cgttaaagcc aaagccgtcc
    15241 atgcgcgcgg cggttttcgt tacctgcaac ctgctaacga tgaaccctgg ctgggtattc
    15301 tggctggcat cagtaccgat gatggttcac aagcctggcg ctatttcccg gaaaacttga
    15361 tgggtaaaga cctggttgat tatttaagtg gtgcgattca gggcggtgaa gcggataacg
    15421 cgacgctggt ttatggtggc aatccgcaac tcttccccta taaacacaac gaaggtcagt
    15481 ttgaagtgct ggtgccgctg cgcaacgcga agtttgcctt ccagccggac tggcctgcat
    15541 taactaacct tgatattgaa ctggacttta ttaacgacgg tttatggatg aaaaccgatg
    15601 gcgttaatct gggcggcgtg cgcgcgagta atctcaccgc agtgatccct gactactcaa
    15661 aagaaaaact gctgattgac gctgacatta aaggtccggg taaagccgta ggcccttact
    15721 ttgatgagac accgctgaaa gattccctgg gtgcgaccct gcaagaactc cagctcgacg
    15781 gcgatgtgaa tgctcgctta catcttgata tcccgctgaa cggcgagctg gtaaccgcga
    15841 aaggtgaagt gacgctgcgt aataacagtc tgtttatcaa accactcgac agcaccctga
    15901 aaaatttgag cggtaaattc agctttatca atggcgatct gcaaagtgaa ccactgacag
    15961 caagctggtt taatcagccg ttgaacgtgg atttttccac caaagaaggg gcaaaagcct
    16021 accaggttgc ggtgaatctc aacggtaact ggcaaccggc gaaaaccggc gttttgcctg
    16081 aagcggtgaa cgaagcatta agtggcagcg tggcgtggga tggtaaagtg ggcattgatc
    16141 tgccttatca tgctggcgcg acctataacg tagagctgaa cggcgatctg aagaatgtga
    16201 gtagtcactt accttcaccg ttagccaaac ctgcgggtga accactagcg gtaaacgtta
    16261 aggttgatgg caatctcaac agctttgaat taaccggaca ggctggtgcg gataaccatt
    16321 tcaatagccg ctggttgctt ggtcaaaagc tgacgctcga tcgtgctatt tgggcggcag
    16381 acagtaaaac gctcccgccg ttgccggaac aaagtggcgt tgaactcaat atgccgccga
    16441 tgaatggtgc cgagtggctg gccctgttcc agaaaggcgc tgcggagagt gtcggtggtg
    16501 cagcgagttt cccacaacac ataacgttac gtacgcctat gttgtcactg ggaaatcagc
    16561 aatggaataa cctgagtatt gtttcgcaac cgacggcaaa tggcaccctg gttgaggcgc
    16621 aagggcgtga aatcaacgcc acgctagcga tgcgtaataa cgcgccgtgg ctggcgaata
    16681 tcaaatatct ttattacaac ccgagcgtgg cgaaaactcg tggtgattca acgccgtcat
    16741 cacctttccc gacaacggag cgcattaact tccgtggctg gccggacgcc caaatacgat
    16801 gcacagagtg ctggttctgg gggcaaaaat tcggtcgcat tgacagtgat atcaccattt
    16861 ctggcgatac gttaacgctg accaatggac tgattgatac tggtttctcg cggcttactg
    16921 ccgatggtga atgggttaat aatccgggga atgaacgtac ctcgctgaaa ggaaaactgc
    16981 gcgggcagaa aattgatgcc gccgcagaat tttttggtgt cacgacgccc atacgccagt
    17041 cgtcatttaa tgtggattac gatttacact ggcgcaaagc accctggcag ccagatgaag
    17101 cgacgttgaa tggcatcatt catactcaac tgggtaaagg cgaaattacc gaaatcaata
    17161 ccggacatgc cgggcaattg ctgcgcttat tgagcgtaga tgccctgatg cgtaagctgc
    17221 gttttgattt cagagacact tttggcgaag ggttctattt tgactccatt cgcagcaccg
    17281 cgtggattaa agacggcgtt atgcacaccg acgacacgct ggtggatggc ctggaggcgg
    17341 atatcgccat gaaagggtcg gtaaatctgg tacgtcgcga cctgaatatg gaagcggttg
    17401 tcgcaccaga gatttctgcg acggtgggcg tggctgcggc ttttgcggtt aaccccattg
    17461 ttggcgcggc agtgtttgcc gccagtaaag tgctggggcc gctgtggagc aaagtctcca
    17521 ttttgcgcta tcacatttcg ggtccgctgg acgatccgca aatcaacgaa gtgttgcgcc
    17581 aaccgcgtaa agaaaaagcg caatgatttg acgagggcgc gtaattgccc caatctcata
    17641 tgataatcgt tgccaaaggc caacgagcca gaacataacc gtaggtcgga tagggcgttc
    17701 acgccgcatc cggcagccgt aaaaaatcct ctactgcagt aactaacgag tagcaaaaac
    17761 gatgagtctt aacctggtaa gtgaacaatt gctagcggcg aacggcctga aacatcagga
    17821 cttgttcgcg atcctcggtc aactggccga acgtcgcctt gattatggcg atctctattt
    17881 tcagtcgagc tatcacgaat cctgggtttt agaagaccgc attattaaag atggttctta
    17941 caacatcgat cagggcgttg gtgtgcgtgc aatcagtggt gaaaaaaccg gatttgctta
    18001 cgctgaccaa atcagcctgc tggcgctgga acagagtgcg caagcggcgc gcaccatcgt
    18061 ccgtgatagt ggtgatggca aagtacagac gctgggcgcg gtagagcata gcccgttgta
    18121 tacctcggta gatccgctgc aaagcatgag ccgtgaagag aagctggata tcctgcgtcg
    18181 cgtcgataag gttgcccgcg aagcggacaa gcgcgtacaa gaagtgactg ccagcctcag
    18241 cggcgtttat gaattaattc tggttgcggc caccgacggc acgctggcgg cggatgttcg
    18301 tccgctggtg cgtctttccg tgagcgttct ggtcgaagaa gatggcaaac gcgaacgcgg
    18361 tgccagtggc ggcggcggtc gttttggtta tgagttcttc cttgccgatc ttgacggcga
    18421 agtccgtgcg gatgcatggg caaaagaagc agtgcgtatg gcgctggtca atctttctgc
    18481 cgttgctgca ccagcgggca ccatgccggt agtacttggc gcaggttggc cgggcgtgct
    18541 gttgcatgaa gcggtaggtc acggtctgga aggcgacttc aaccgccgcg gcacttcagt
    18601 atttagtggg caggtcgggg agctggtggc ttcagaactg tgtaccgtgg ttgatgacgg
    18661 cacgatggtc gatcgccgtg gttcggtggc gattgatgac gaaggtacgc caggccagta
    18721 caacgtgctg attgagaacg gcattctgaa aggctacatg caggataaac tcaacgcgcg
    18781 tttgatgggg atgacgccga ctggcaacgg tcgccgtgaa tcctacgccc atctgcccat
    18841 gccgcgtatg accaacacct atatgctgcc gggtaaatcg accccgcagg aaattattga
    18901 atccgttgag tacggtatct atgcaccgaa ctttggtggc ggtcaggtgg atattacatc
    18961 cggcaaattc gttttctcca cttcagaagc gtatctgatt gaaaacggta aagtaacgaa
    19021 gccggtgaaa ggcgcgacgt tgattggttc cggtatcgaa accatgcagc agatttcgat
    19081 ggttggcaac gacctgaaac tggataacgg cgtgggtgtc tgcggtaaag aagggcaaag
    19141 tttgccggtt ggcgtgggcc agccaacgct gaaagttgat aacctgactg ttggcggtac
    19201 tgcgtaataa tttcattatt ttcagaagga taattaatct ttctacgtgc acgaacggtc
    19261 ccctcgctcc tctggggtta gggtgagggg aacccgttgg cacaggtttg tagaacgtaa
    19321 cagtacaata tgaattactt ctctttcccg cgcccgtgca tctcctgaaa caatttaccg
    19381 acctcaacaa aataatccgt cagcgagttg atcacgacct gtaccttcag cggcagctta
    19441 tctttttcgg tatataacgc ataaaccggg cgtggatctg actggtaacg tggtagcagg
    19501 atctccagct ccccgcgatt gatctcgttg atcacccaca tcagcggcac gtaggcgatc
    19561 ccagcgcctg cagtcagcca gcgcaccagc gtcatcggat cattagtcac aaatctcccc
    19621 tgcgggatca ggcgagtcga gatcccttcc ggtgcgatca gttcaaattc attgtcgggc
    19681 cgcacgctgt attcaagcca tgaatggcta cttaaatcgg cgggtttttc cggtatgccg
    19741 tattgtgtga gatagctttt tgcggcgcac accaccattg gcatcgcgcc cagacggcgg
    19801 gaaaacaggc tggaatcctg caacgcgccg acgcggatca ccacatccag accgtcggca
    19861 atcaggtcgg gggcaggaat tccggtaacc agattgacgc tcaaacctgg gtattctttc
    19921 agcatttttg ctgtcagccc ggcgagaaca ttttgtgcca tagttgaaga acagccaatg
    19981 cgcagcgtcc cgatgggggt gttattgaag gcatacagtt gctcatgaac atcctgcact
    20041 tcatgaagca tacgacggca gccctggtag taaattctac cggcttcggt caggccaatg
    20101 ctgcgtgtgc tacggtttaa cagctttacc tgcaactcat cttccagttt tgacaccgtc
    20161 tgactgatgg acgaaacgct catctgtagc tgtctggcgg cggcggtaaa agagccaaat
    20221 tcaactactt tggcaaacac cgacatgcgt tttagtcgtt ccattattca ctctgactta
    20281 aaagtgattt agatcacata atatagataa cagcataaca gttacgctaa tatattaaat
    20341 atcaatctac agcaatgttg ctctcgcccg gctttccatg cccttctctg cggcgacaga
    20401 tgctgaaaat aataacgcct gctctctctt tacaaccaag gtcaacatga gtctgtttcc
    20461 cgttatcgtg gtgtttgggc tgtccttccc accgatattt tttgaattgc ttttatcact
    20521 ggcgattttc tggctggtgc gccgggtact tgtgccaaca ggtatctacg actttgtctg
    20581 gcatccggcg ttgttcaaca ccgcgctcta ttgctgcttg ttttatttga tatcgcgact
    20641 gttcgtttga ggttgaagtg aaaacactaa taagaaaatt ctcccgtacg gccatcacgg
    20701 tcgtattagt cattctggcc ttcatcgcaa tttttaatgc ctgggtctat tacaccgaat
    20761 ccccctggac gcgtgacgcg cgctttagcg ctgacgtcgt tgcgatcgcg ccggacgttt
    20821 ctggactcat tacccaggtg aatgttcatg ataaccagct ggtgaaaaaa ggacagatac
    20881 tgttcaccat cgaccagccg cgctatcaaa aggcgcttga ggaagcgcaa gccgatgttg
    20941 cttattatca ggtactggca caggagaaac gccaggaggc cggacgtcgt aaccgtctcg
    21001 gtgtgcaggc gatgtctcgc gaagagatcg accaggctaa caacgtacta caaacggttc
    21061 tgcatcagtt agcgaaagcg caggcgaccc gcgatctggc aaaactggat cttgaacgca
    21121 cggtgatccg cgcgccagca gatggctggg tgaccaacct caacgtctat accggtgagt
    21181 ttattactcg aggatcaacg gcggttgcgc tggtgaaaca gaactccttc tatgtactgg
    21241 cctatatgga agaaactaag ctggaagggg tgcgtccggg gtatcgtgca gagatcacgc
    21301 cgcttggcag taacaaagtg ctgaaaggga ctgttgatag tgttgccgca ggggtcacca
    21361 acgccagcag cacgcgtgac gacaaaggga tggcgactat agactctaac cttgaatggg
    21421 tgcgtcttgc gcaacgtgtt ccggttcgta ttcgtctcga caaccagcaa gagaacatct
    21481 ggcctgcggg caccactgct acagtggtgg tcactggcaa acaagatcgc gacgaaagcc
    21541 aggattcgtt cttccgtaaa atggcccatc gcctgcgtga gtttggttaa tcacgatggg
    21601 tattttctcc attgctaacc aacatattcg ctttgcggta aaactggcga ccgccattgt
    21661 actggcgctg tttgttggct ttcacttcca gctggaaacg ccacgctggg cggtactgac
    21721 agcggcgatt gttgccgccg gtccggcctt tgctgcggga ggtgaaccgt attctggcgc
    21781 tattcgttat cgtggctttt tgcgcatcat cggcacattt attggctgta ttgccggact
    21841 ggtgatcatc attgcgatga tccgcgcacc attattgatg attctggtgt gctgtatctg
    21901 ggccggtttt tgtacctgga tatcctcgct ggtacgaata gaaaactcgt acgcgtgggg
    21961 gctggccggt tataccgcgc tgatcattgt gatcaccatt cagccggaac cattgcttac
    22021 gccgcagttt gccgtcgaac gttgtagcga gatcgttatc ggtattgtgt gtgcgattat
    22081 ggcggatttg ctcttttctc cgcgatcgat caaacaagaa gtggatcgag agctggaaag
    22141 tttgctggtc gcgcaatatc aattaatgca actctgtatc aagcatggcg atggtgaagt
    22201 tgtcgataaa gcctggggcg acctggtgcg acgcaccacg gcgctacaag gcatgcgcag
    22261 caacctgaat atggaatctt cccgctgggc gcgggccaat cgacgtttaa aagcgatcaa
    22321 tacgctatcg ctgacgctga ttacccaatc ctgcgaaact tatcttattc agaatacgcg
    22381 cccggaattg atcactgata ctttccgcga attttttgac acgccggtag aaaccgcgca
    22441 ggacgtccac aagcagctca aacgcctgcg gagagttatc gcctggaccg gggaacggga
    22501 aacgcctgtc accatttata gctgggtcgc ggcggcaacg cgttatcagc ttctcaagcg
    22561 cggcgttatc agtaacacaa aaatcaacgc caccgaagaa gagatcctgc aaggcgaacc
    22621 ggaagtcaaa gtagagtcag ccgaacgtca tcatgcgatg gttaacttct ggcgaaccac
    22681 actttcctgc attctgggca cgcttttctg gctgtggacg ggctggactt ccggcagtgg
    22741 tgcaatggtg atgattgcgg tagtgacgtc actggcaatg cgtttgccga atccacgcat
    22801 ggtggcgatc gactttatct acgggacgct ggccgcgctg ccgttagggc tgctctactt
    22861 tttggtgatt atccctaata cccaacagag catgttgctg ctgtgtatta gcctggcagt
    22921 gctgggattc ttcctcggaa tagaagtaca gaaacggcga ctgggctcga tgggggcgct
    22981 ggccagcacc ataaatatta tcgtgctgga taacccgatg actttccatt tcagtcagtt
    23041 tctcgacagc gcattagggc aaatcgtcgg ctgtgtgctc gcgttcaccg ttattttgct
    23101 ggtgcgggat aaatcgcgcg acaggactgg acgtgtactg cttaatcagt ttgtttctgc
    23161 cgctgtttcc gcgatgacta ccaatgtggc acgtcgtaaa gagaaccacc tcccggcact
    23221 ttatcagcag ctgtttttgc tgatgaataa gttcccaggg gatttgccga aatttcgcct
    23281 ggcgctgacg atgattatcg cgcaccagcg cctgcgtgat gcgccgatcc cggttaacga
    23341 ggatttatcg gcgtttcacc gacaaatgcg ccgcacagca gaccatgtga tatctgcccg
    23401 tagcgatgat aaacgtcgtc ggtactttgg tcagttgctg gaagaactgg aaatctacca
    23461 ggaaaagcta cgcatctggc aagcgccacc gcaggtgacg gaaccggttc atcggctggc
    23521 ggggatgctc cataagtatc aacatgcgtt gaccgatagt taagtcaaaa ccgacgccaa
    23581 aagcgtcggt tttttcatgg ctatacttag cgatgaacgg cagaactcgc cgcgaaacgt
    23641 gacggtggca acagatgaat atttatacct ttgattttga tgagattgag agtcaggagg
    23701 atttttatcg cgacttcagc caagcctttg gtctggcgaa agataaggta cgcgatctcg
    23761 actcactatg ggatgtgtta atgaacgatg tcctgccgct accacttgag attgaatttg
    23821 ttcatctggg agagaaaacg cgtcgccgtt ttggcgcgtt aatattgctg tttgatgagg
    23881 cagaggaaga gctggaaggg catttgcgtt ttaatgttcg tcattagcgc aaaaaaaagc
    23941 ccccgaaccg ggggcaatat cgtcggacaa gacgatgagg gtttatttgt acagttcagc
    24001 cgtagcgtgc caggtgtcac cgctacgagc ttcagtaatc tggtaggccg ttgcgccttt
    24061 ctcttccgct tttttgttca gcatttcacg tatatccatt ggcgaagacg ccacaccact
    24121 tacggatacg gtcccgattg cttcacggtt ttgtgcttgt gcagcatcaa tggagtcggc
    24181 agcgaatgca ccgaaagaga gaacagaaag tacgcttaat gcagcaacag tggttttgat
    24241 tttcatgatt tttacctcga cataatcttt ttgctgggtc tttgtttcgt gacccttatc
    24301 acaa
//
LOCUS       DAJOWB010000068        23742 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJOWB010000068 DAJOWB010000000
VERSION     DAJOWB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.068"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..684
                     /locus_tag="OHO85_002890"
     CDS             1..684
                     /locus_tag="OHO85_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCQ2672237.1"
                     /translation="MTPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQAGN
                     VGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRR
                     RGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIK
                     LNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAIT
                     DFMTARGYH"
     gene            complement(733..2232)
                     /gene="pitB"
                     /locus_tag="OHO85_002891"
     CDS             complement(733..2232)
                     /gene="pitB"
                     /locus_tag="OHO85_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCQ2672238.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(2524..4383)
                     /gene="gss"
                     /locus_tag="OHO85_002892"
     CDS             complement(2524..4383)
                     /gene="gss"
                     /locus_tag="OHO85_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCQ2672239.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            4588..5454
                     /gene="yghU"
                     /locus_tag="OHO85_002893"
     CDS             4588..5454
                     /gene="yghU"
                     /locus_tag="OHO85_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCQ2672240.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(5577..5825)
                     /gene="hybG"
                     /locus_tag="OHO85_002894"
     CDS             complement(5577..5825)
                     /gene="hybG"
                     /locus_tag="OHO85_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCQ2672241.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(5838..6179)
                     /gene="hypA"
                     /locus_tag="OHO85_002895"
     CDS             complement(5838..6179)
                     /gene="hypA"
                     /locus_tag="OHO85_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCQ2672242.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(6172..6660)
                     /gene="hybE"
                     /locus_tag="OHO85_002896"
     CDS             complement(6172..6660)
                     /gene="hybE"
                     /locus_tag="OHO85_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCQ2672243.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(6653..7147)
                     /locus_tag="OHO85_002897"
     CDS             complement(6653..7147)
                     /locus_tag="OHO85_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCQ2672244.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(7147..8850)
                     /gene="hybC"
                     /locus_tag="OHO85_002898"
     CDS             complement(7147..8850)
                     /gene="hybC"
                     /locus_tag="OHO85_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCQ2672245.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(8847..10025)
                     /gene="hybB"
                     /locus_tag="OHO85_002899"
     CDS             complement(8847..10025)
                     /gene="hybB"
                     /locus_tag="OHO85_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCQ2672246.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(10015..11001)
                     /gene="hybA"
                     /locus_tag="OHO85_002900"
     CDS             complement(10015..11001)
                     /gene="hybA"
                     /locus_tag="OHO85_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCQ2672247.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLLGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(11004..12122)
                     /gene="hybO"
                     /locus_tag="OHO85_002901"
     CDS             complement(11004..12122)
                     /gene="hybO"
                     /locus_tag="OHO85_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCQ2672248.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(12311..12598)
                     /locus_tag="OHO85_002902"
     CDS             complement(12311..12598)
                     /locus_tag="OHO85_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCQ2672249.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(12717..13604)
                     /locus_tag="OHO85_002903"
     CDS             complement(12717..13604)
                     /locus_tag="OHO85_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCQ2672250.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            13761..14801
                     /gene="gpr"
                     /locus_tag="OHO85_002904"
     CDS             13761..14801
                     /gene="gpr"
                     /locus_tag="OHO85_002904"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCQ2672251.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(14841..15335)
                     /locus_tag="OHO85_002905"
     CDS             complement(14841..15335)
                     /locus_tag="OHO85_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCQ2672252.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            15526..16410
                     /gene="yghA"
                     /locus_tag="OHO85_002906"
     CDS             15526..16410
                     /gene="yghA"
                     /locus_tag="OHO85_002906"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCQ2672253.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(16682..17107)
                     /gene="exbD"
                     /locus_tag="OHO85_002907"
     CDS             complement(16682..17107)
                     /gene="exbD"
                     /locus_tag="OHO85_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCQ2672254.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(17114..17848)
                     /gene="exbB"
                     /locus_tag="OHO85_002908"
     CDS             complement(17114..17848)
                     /gene="exbB"
                     /locus_tag="OHO85_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCQ2672255.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            18100..19287
                     /gene="metC"
                     /locus_tag="OHO85_002909"
     CDS             18100..19287
                     /gene="metC"
                     /locus_tag="OHO85_002909"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCQ2672256.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEEQANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            19427..20086
                     /locus_tag="OHO85_002910"
     CDS             19427..20086
                     /locus_tag="OHO85_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCQ2672257.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(20126..21025)
                     /gene="yqhC"
                     /locus_tag="OHO85_002911"
     CDS             complement(20126..21025)
                     /gene="yqhC"
                     /locus_tag="OHO85_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCQ2672258.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            21219..22382
                     /gene="yqhD"
                     /locus_tag="OHO85_002912"
     CDS             21219..22382
                     /gene="yqhD"
                     /locus_tag="OHO85_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCQ2672259.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWGATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            22487..23314
                     /gene="dkgA"
                     /locus_tag="OHO85_002913"
     CDS             22487..23314
                     /gene="dkgA"
                     /locus_tag="OHO85_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCQ2672260.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            23514..>23742
                     /locus_tag="OHO85_002914"
     CDS             23514..>23742
                     /locus_tag="OHO85_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672261.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGW"
BASE COUNT         5921 a         6327 c         5924 g         5570 t
ORIGIN      
        1 ataacacctc cattaattgc cgttattggt agcgatggtt caggcaagtc aacggtgtgt
       61 gaacatctta ttaccgttgt cgaaaaatat ggtgctgccg aaagagttca tttaggaaaa
      121 caggccggaa atgtcggtcg tgcagtgaca aaattaccgt tgatgggaaa atccttacat
      181 aaaacaattg aacgaaatca ggtgaaaaca gcaaaaaaat tgcctggacc agttccggcg
      241 ctggtaatta cagcgtttgt cgcccgtcgc ttactgcgct ttcgtcatat gcttgcctgt
      301 cgtcgtcgcg ggttaattgt tctaaccgac cgttatcctc aggaccaaat tcctggcgct
      361 tacgatggta cggtgttccc acctaacgtt gaaggtggtc gttttgtctc atggctggca
      421 agccaggaac gtaaagcgtt tcactggatg gcgagccata agcctgatct ggtcatcaaa
      481 ctcaatgttg accttgaagt tgcctgtgca cgtaaacccg accataaacg ggaatcgctg
      541 gcgaggaaga ttgccataac gccacagtta acctttggtg gtgcacaact ggttgatatc
      601 gatgccaatc agccactgga acaggtgttg gttgatgcag aaaaagcgat tacggatttt
      661 atgaccgcgc gtggttatca ctagtcaaaa atggaaatgc ccgatcgcca ggaccgggca
      721 ttttcaggaa ggttaaatca actgcaatgc tatccagtac agcccaccag aaagaaaaat
      781 tgccgccggt aaagtaaata cccacgccat caggatgctg gttaccgttt tacgctgtaa
      841 cccaccgccg tccaccacca tcgtccctgc aactgcagac gagaggacgt gtgttgtgga
      901 gacgggcatc ccaatataac tggcaagacc gatagacact gccgccgtca tttgtgccgc
      961 catgccttgc gcatacgtca tgccgcgctt accaatcttc tcaccgatgg tcatcgctac
     1021 acgacgccag ccaatcatgg tgccaatgcc gagcgccagt gctaccgcca tgatgatcca
     1081 caccggagcg tactcaatgg tgcttaacat atcgctgcgc agttttttca gcaggttctg
     1141 gtcttcttta ctgacgcctg gcagtttcgc tagcttcgcg gaggtatcag agatgcacag
     1201 cataatgcgg cgcagctggc tgcgctgact cacgcttaac ggctcgtaac tttccatatt
     1261 gcctggcagc atcgttttaa cgcgcgcaat cgcatcaaag gtatttgccg gatgacagtg
     1321 aaactctgtt acttgcgtgc catcagtcga tgctgcaggc aatggaggtt ccatcgcaat
     1381 caacttctgc ggcagttcag gatgctgttg caggtagtgt tcgaagttgg tgacggcatc
     1441 gcgggtacgg gtaatttcat agccggacgc gttcatattg acgacgaagc cagcaggggc
     1501 aatccccacc agtaccagca tcaccaggcc gatccctttt tgtccgtcgt tcgcgccgtg
     1561 cgaaaacgcc acgcccgcag cggaaacaat cagcgcaata cgcgtccaga atggcggttt
     1621 acgtttgcct tttttctttt tgcgatcttc cggaatgcgg tgaatacggt cacgcttttt
     1681 cgtcccgctc cagtagcgtc gcagcaggaa tatcaggcct cccgcaatga ccaggccgac
     1741 gataggggaa acaatcagcg aggagaaaat tttggtcact tcacgcaggt ttaacgcatc
     1801 catcaccgat gagccggtta acagcgcgtt ggttaaaccg atgccgataa tcgcaccaat
     1861 caaggtgtgc gaactggagg ccggtaaacc gaagaaccac gttcccaggt tccagataat
     1921 cgccgccagc agcatggaaa agaccatcgc caggccgtgg gttgacccca tattcagcaa
     1981 caaatcggtt ggcaacatat ggacaatggc ataggcaacg ctaagtccgc ccaataacac
     2041 gccaaaaaag ttaaaaaatg ccgccatcac cacagcaagt tgtggttgca tggcacgagt
     2101 ataaataacg gttgccaccg cattcgccgt gtcatgaaaa ccattgattg cttcgtagaa
     2161 caacacaaat gccagagcaa gcaataacaa aagccctgtg tatatatcaa ggccaacaaa
     2221 taaatttagc atatattaga ttacgccatt ttgaatttac gaacggacgc attatcagtg
     2281 actttaacgg catgggcaaa gtgaaatatc attttttaac gataaaaaga gtgttatttc
     2341 ttgtgtatca gacaataagc attcattcaa aacctttaga aataatcaaa acacaggtat
     2401 tccactggtt aatatattca catatgaaat gaataaatat tggaatatat aaatattgaa
     2461 tattttgatt aatccccgta ctgattattc ttcataatca gtacggggtt gctacaacat
     2521 gtattacttt ttcaccacaa ttaacggttc aatatcactc tcttttttga tgaccagtga
     2581 ttcatcaccg cgcaaacacg tcccaccgta gttgccgcca acggtgaagg tacatacctg
     2641 aatgtattta ccgtccactt tcggcaaaca ccacagttgc tgatagatgt ttttctgctc
     2701 ggcaaattta ccgctggttt tgtccagcac ctcttcatga tggctgacga ggtcgatatt
     2761 gctgccacag cgaccggcga tcggtttcac tgcgtaacct gttttcacca gttcatcatt
     2821 aacagtgaaa tcggtatcca gcaggtaacg atggtgcggg aacagcgacc agaggatcgg
     2881 cagaatcgct ttgttgccgg ggatcaccgt ccacagcggc tcaaagacca gcacttccgg
     2941 gcgcagcaat acgtcgataa gacgcacttc gttttgcgga tgaccggtac ggattggcac
     3001 cgcagcaaac tcacggtcgc taacttcacg aatctgatca aacgcggttt cccacgccca
     3061 ggttttccac acgcagttaa ccagccgtcc ttccccatca atcagttgcc cggcagcatc
     3121 ccagcccagt tcatccagcc cgcgcaagat acgcgtttca aagcccgcct ggtgcagcgc
     3181 ctgctccata aactgcgcgt gatagttttc ctcgatatct ttgtcctgca tgatatggac
     3241 aaacggacgt gcacgactgt gtttccaggc accagccagt tcgttaatca gcccttccgc
     3301 cggattgaag ccgttgcctt tatagccctg ctccgcccaa cgttcgagga tcaagcccgc
     3361 ttcggtatga catgaggcag agtcggcgtt gtattcgtaa accttcaggc cacgctcatc
     3421 catgcagaaa tccatacgac cagtgatcat atggtgacgg cgacgctgcc aggagagacg
     3481 caaacgtggc cagaggattt tcgggatgtc gaacagcgcc agcaggttgt catctttcag
     3541 caccttgtcg gttgcgtgaa gatacatcag gtgcagctcg ttggtggctt taattagctc
     3601 ctgctcggca ctctcggtaa tggtgtagta atgataagga tcctgattga tcacctgacc
     3661 gttggcctgc acataggcgt tttgcagcgg atctttttca tccagccatt taccgtcaaa
     3721 ctggcctttg ttttccaggc gcgctccgct gattttcagc agctcgcctg caatttccgg
     3781 ctgcggtaag ctgtattcag tatcttccgt ctggatcatc cagcccagaa tggtggtgtc
     3841 atcaaaagtg tctttcaggg tatagcagcc gttttcgacc accatctcca gctcacgcgt
     3901 ccactgttgc ccttgcggca acggggaatg aatcacgttc tgttccgcaa tacggacttt
     3961 gttgccatgc aattgggtaa tgatggcgac atggccagtg tctttaaatt caccgccttt
     4021 atcccagata agaagcgcac ccgcgaccgg cgcacgcggc gagccgttag gaaatgcctg
     4081 caatggcagg atgttgtcat taaccacttc tcgcaggaag cgcagcgaga aaatctccca
     4141 cgccataccc acgtcagtaa agaccacacc gtaattcaga aagagaaaac ggcgagcaaa
     4201 ttcaacgcat tgccacttgt ggcccatata ttcgtcgtcg atatagctac ggaatacggc
     4261 gtcatcttcg tattcctgcg gatcgagaga actgtaatct gaagagtaga ttgctacccc
     4321 acctggggcg tagcccaata atgtcccgaa cggggcatcc tggctggtcg ttcctttgct
     4381 catcacttta cctttaacaa tacaacctaa gcagttggcg taattgttgt gctctgatta
     4441 cctgctcagc gacattaacc ggacagaggt cagactaatc atacacctca tcgcagcggc
     4501 tggcgcaaag ggttaaaaaa ttcacattct gtacagcaag tgacctgcta cactgcttca
     4561 acactaccac tcagaaggca actcactatg acagacaata cttatcagcc cgcgaaagtc
     4621 tggacgtggg ataaatccgc tggcggcgcg ttcgccaata tcaatcgccc ggtttctggt
     4681 ccgacgcatg aaaaaacgct gcccgttggc aaacacccat tgcaacttta ttcgctggga
     4741 acgccgaacg gtcagaaagt aacgattatg cttgaggagc tgctggcgct gggcgttact
     4801 ggtgcagagt acgacgcctg gctgattcgt attggcgatg gcgatcaatt ctccagcggc
     4861 tttgtcgaag tgaacccaaa ctcgaagatc ccggcgctgc gcgatcatac gcataatccg
     4921 ccgatccgcg tgtttgaatc tggttcgatc ctgctttatc tggcggagaa atttggctac
     4981 ttcctgccgc aggatttggc aaagcgtact gaaacgatga actggctgtt ctggttacag
     5041 ggcgcggcac cgttcctcgg cggtggtttt ggtcactttt accattacgc accggtaaag
     5101 attgagtacg ccatcaaccg ctttaccatg gaagccaaac gtctgctcga cgtgctggat
     5161 aagcaactgg cgcagcataa gtttgttgcg ggcgatgagt acaccattgc ggatatggcg
     5221 atttggccgt ggtttggcaa cgtggtgtta ggtggtgtgt atgatgccgc tgagtttctt
     5281 gatgcgggca gttataagca tgtacaacgc tgggcgaaag aagtaggcga acgtccggcg
     5341 gtgaaacgtg ggcgtattgt taaccgcacc aacggaccgc tgaatgagca gttgcatgag
     5401 cgccatgacg ccagtgattt cgagacgaat acggaagata agcgtcaggg gtaagggttg
     5461 gtgttcgtcg cagcaagcca tccaggccgg ataaggcgtt cacgccgcat ccggcaatcg
     5521 tgcacaatgc ctgatgcgac gctgccgcgt cttatcaggc ctacgaaagc taatcatcac
     5581 gcgctggtaa tgtcgtaatc catttggcgc agtgcgtcta atgtggcttt ggcttcatct
     5641 tcgtcgatga tgctcatggc aaatccgacg tgtaccagca cccactggcc cagtagatcg
     5701 gcagggttac cttcacaaat cagggcgata ttcacgtcgc gcttgatacc acatacttca
     5761 acctgcgcaa gctggtgaat atcttcaccg acagccagca cctggcctgg aacgccaata
     5821 cacattatta actccggtta ttcaacttca atacttttga cgatcagcga atcgccggta
     5881 tcgacacgca accgctcgcc gtgacagagc ggacactgcg catcgtgctg atgaatctcc
     5941 accacctggc tgcaatccca gcaccaggcc tgggcgggtt tatagacgat atgtaaatcg
     6001 cacccttgcg ccaccgttcc gtggcagaca atttcaaaac taaaacggac ggcgctctcc
     6061 tcaacgcagg agagcgcgcc aatttccagc cacacggcgg tgacgcgctt aacatcgtgc
     6121 tgctccgcct gccgttggat aatttcaacg gcgctctggc aaagagacaa ctcatgcatt
     6181 ttcgccactc ctgcgaccaa acagcagggc gcgacgccct gcgtgtggta catccggatt
     6241 cgtgactggc agcgaaagga tcattcgtgc gcagtcatcc gtcaggcgtt ggccctcttc
     6301 aatcgacatg ctgtgcgaaa gcggcgacat cagcgaacag gagagatatt gcgaaacacc
     6361 gtccagttcg ccaacggtaa aggtcatagt gccatacggc agttgcagac caattttttc
     6421 actcactttg cgcagcggcc agagttgatc cgggccgggg aagataactg cactcagcat
     6481 ccacggggtg atcacacacc ccgtccactg accttcgaac agcgtaaaat cagaaacata
     6541 caccggcatt gaaggatgca gaaaagagag atcgtgcatc gaacgccggg caatttcttc
     6601 aaacgctgcc tgtacttgcg ccttcgggga ggtctggaaa cctgctatct cttcagtcat
     6661 gaatcgcctc ccgtgggatg gcttccacgc cagattcacg cagcgcagcc agaacctgct
     6721 caagcgcagg ttcaatcatt gcttcaaccg tcggcgttaa gccgatgtgt ggctccagcg
     6781 attccgggat cacgccgacc agggtgagct ttttcggaaa ctcaccggtg aagcgcaggg
     6841 ccgacaagac gtcggccagg ccaagctgat gcggagagat tttgttggta aacaacgccg
     6901 gaacttcttc atcgcgcagg atcatcatcg ttcccggcgc gctcttcttc gacacaatgg
     6961 catccgcaat aatcagatga tcgcgatttg ccatgtcgcc aagcagctcc attcccgccg
     7021 tgccgccatc gaggatctca acataatccg gcagaatgta tcgttgctct aacgcttcga
     7081 caatccgcac accgatggct tcatcggtca gcaaaatatt gccgacccct aagactaaaa
     7141 tacgcattac agaaccttca ctgaaaccac ttcgttgccg tcagcatcca ctacgtgtac
     7201 cgcacaggcc atgcacgggt cgaaggagtg aatggtacgc accacttcca gcggtttatt
     7261 cggatcggca accggtgtac ccaccagcga ctgctcgtaa ggaccgacgt catcattgaa
     7321 gttacgcgga ccagagttcc aggttgatgg aacaaccgcc tggtagttac tgatgatacc
     7381 gtctttgatc accatccagt gagagagcat accgcgcggc gcttcgagga agccaacacc
     7441 tttgaactca cccgttgccg gaatgttcgg cttcacaaag gtggtatgat cgcctttacc
     7501 gatattggtg atcagtgcac tgtattgatt ttgcaggata tcctgcaatt cgcagcagtg
     7561 aacggtacga ccaataatac ggcccagcgt agagtgcagt tgcgccactt caagcgtgtt
     7621 accagtcagt ttctgataaa tcgcaacgat ttcattcagt ttgttctggg tagactcgcg
     7681 tcctgccgcc agtttcacca gcatgttagc cagcggaccc acttctaccg ttttgccgta
     7741 gaaagtcggt gatttcaccc aggaatattt cccgtcgtca gaccaaccat cataagccgg
     7801 aatggtggta ccttcccacg gtgcctgcgg cgcttcgtct ttataccagg agtgcttcgc
     7861 gctttcctga atgcctttga tcaggtattc atcggaatga gaagtgatcg gacgatacga
     7921 ggacagatcc gcattctcaa tgtagccgcc cgggaacagg aagctgccgt ttttactgtc
     7981 ggtcgggaat tccggcacgc tcaggtagtt caccgcacct ttaccgcgcg tcagccattc
     8041 cgggtagaac gcggcaataa ctgcggtatc aaccttataa acctgttcaa caaagtcgct
     8101 cagcttgtcg atgaaagact tgatgtacat caggcgctca aggttcagca cgcccaaacc
     8161 gtcgaggttg atcgggttcg cgacaccacc taccgccagg ttctgaatgt gcggcgtttt
     8221 accgcccagc agcgccacga cgcggttagc gtcacgctgg cactccagcg cctgcaggta
     8281 gtgcgctacc gcaatcaggt tcacttccgg cggcagtttc atcgccgggt gaccccagta
     8341 gccattggcg aaaataccca actgaccgct ggcaaccaga tctttgatct tgttctgaac
     8401 tttggtgaac tcttccggac tgttcaggtg ccaggtcgaa acgcctttca gcatttcgga
     8461 ggctttggtt gggtcagctt gcagtgcaga agtgatgtcc acccagtcca gcgccgaaag
     8521 ctgatagaaa tgaacaatat ggtcatgcgt ggtgtgcgca gccagaatga tgttacggat
     8581 gtattgcgcg ttaaccggaa cgtcgatatt cagcgcactt tctgccgcac gaacggaaga
     8641 cagcgcgtga gtggtagtac atacgccaca gatacgttgc acaatcatcc atgcatcgcg
     8701 cggatcgcgg tttttcacga tctcttccat gccgcgccac atggtaccgg aagcccatgc
     8761 tttcgaaacg acgccatttt cgatttcgca atcgatgcgt aaatgcccct caatacgggt
     8821 taccggatca atagtaattc tctggctcat gctttgctcg cctcatgacg attatgatcg
     8881 ttttgtttta aaggaggaag tatcggcagt agacgaatga gtacgatgta agcgcaaatc
     8941 tcaatagcca caaaaccaat agaaatcaac agttcttccc aggtcgggaa gtaggcgtaa
     9001 ccgccgcccg ggttgaatgc caccagcgaa taggtcagac gccaggttgc acaacctaac
     9061 agtgcgctca gtgctgacag gaacagcatg cgggaatcat tacgcagctt cgccacacgc
     9121 agaacgacca gcgggaagag catcagcagg acttcaatcc agaacatcac ggagtagaag
     9181 tcaccggcaa acgctaacga cagcttgtcg cgatagataa gctcgccaaa gcgcagcacg
     9241 atgaaaatcg ccagcaacac actgatggtg ttggtcagct taacaaacag actcttttca
     9301 tccggaccgt tgccacgcag acccgcctgc accagcgaac cttcaaagat gacaatcgag
     9361 aagcccatga tgaacgccgt cagcagcgag aacagcggca acatttcata gctctgccac
     9421 aacggatgca ccttgtagcc cgccgagatc atcagcgacc ccattgaaga ctggtgcatg
     9481 gtcggcagca gcgcaccgag cgcgatgatg aagaacatca ccttgtttag tcgctgtagc
     9541 gacaccttcc accccagacg ttcaaacagt gccggagcaa actccagtgc catcacgccg
     9601 atatagatgg tcatacagac cgccgtctcg aacagtaccg agttcacgtt gaagtggccc
     9661 ggaatgtaga agtacggcag gttccagtag cgacccacgt cgatagtgat cgacaagcca
     9721 cccagtgagt aaccaaacag actcgccaac agcgccggac gcaccagcgg atggtattgc
     9781 ccacggttaa agacgtatac cgcccacgcc agcgcccagc cgccacaggc aaagccggtg
     9841 ccaatcagca ggtcaaacgc gatccacacg ccccacggga agccgccgtt caggtcagag
     9901 acagagccca gaccgaacac cagacgcttc acaatcagga gcatacagat gacgattaac
     9961 ggtccaaaaa tcatgaccgg tttactgatg attttgccgc ccagcggttg tggatcatga
    10021 ctcatgatcg tctcctccgt cgtgatggtc gtttttggtg ttgcgacgaa ccagcacggt
    10081 taagcccgcc agcacagcca gtggtagcat catgccttta tacagggtgt gttgaatatt
    10141 ttcggaacgc gcaccggtag aaagatcgtc cagtttcggc aggtcgagat tttcataagg
    10201 cacacccgtc agtaccagaa cctgagtacc gccgccctct ttctcgccgt acagatgcgg
    10261 ataatatttc ggcaccgtat gcaggtaagt gtcgccagat ttcagcgtct gacgcggata
    10321 gtggtattcg ctgccaggct tcagcgccag acgttttttc gcctccgcca tcagctcttc
    10381 acgcgtaccg aaaatcaccg cgcccgccgg gcacacttct acgcagccaa gcagaccgcc
    10441 tttatcgaga cgttccacac ctttctggtt gcacagctcg cacttatgca gcgcaccaaa
    10501 cgggttgttg tagtcgtact tcggcacgtt gtacggacag gcgaccatgc agtaacggca
    10561 gccggtgcac acgtctttgt cgtaatggac aatgccggtt ttcggatctt ttttcagtgc
    10621 agagaccggg cacacagaga cacagttcgg atcgacgcag tgcatacact gtttcttaat
    10681 gtacgcgtag ccgttctcct cctggtcttt gttgacccct gtgccgctgg tccacacctg
    10741 aatgatgtta ttggtatacg gcgacagttt gtcgttgttc gaccaggtct gttccccttg
    10801 cgggttacgt tcagggaagt tgatatcctg acacttggtg acgcaagcct ggcagcctac
    10861 gcacaaggtc gagtcgtaca acatccccag cgatcccgga attggcgggc ggttttcagc
    10921 agccgcatga ctgacagacg gcagcgcgcc cgtcagcaat gccccgcagg aggctgcttt
    10981 aataaaatta cgtctgttca cggttattct ccccgtgagt cagcgttatc tttcttttgc
    11041 tgacgaccca gttcacgcac cgccatcacg ctgacaccgg caacgagccc aaccacaccg
    11101 ccgagcaaac caatagcgcc tgcagagacg ttgccgccct ctttagcgtt aacatccggt
    11161 ttctgtgaac gcggagtctg attttcgacg ttggcaagct gatggatgcc tttatggaag
    11221 ccgatacctt cttcgttaca gccatagcat gggtgaccaa tcgctaccgg ccacacgccg
    11281 ccaacatcgc agaattgcag cgttgagcag ttgccgtaag tttctggccc tttacagccg
    11341 aggtggtaca ggcaccagcc ttcacggtgg ccttcatcac cgaactcttt ggcaaaacga
    11401 ccagcatcga agtgcgggcg acgttcgcag tgttcgtgaa tcagacggcc ataggcgaag
    11461 gtcggacgat ttttgtcatc cagtttcggc ggtttgccgt aagtgatgat gtgcgcaacg
    11521 gtcgcgagga agttgtgcgg gttcggcggg cagcccggaa tattgataac ggttttgcct
    11581 ggcagaactt cttgcaggct gactgcgcca gttgggttaa ctccagctgc ggcaacaccg
    11641 ccccacgcag agcaggaacc gatagcgata atcgctgctg cgccttctgc cgctttgcgg
    11701 atgtgatcca caatcggctc accagcaacc atgcaataaa taccgttatc ttttaatggg
    11761 atggaaccat ccacaaccaa cacatactgc cctttgtact tctcaagtgc gttatgtttg
    11821 ttctcttcga cctgatgacc gaaggcggcg gaaagcactt cgtgatactc cagagagata
    11881 gtctccagca cgaggttttc taccgttgga tgcgttgcac gaagcagaga ttccgtacaa
    11941 ccggtgcact cctgcgcgcc aatccagata actggcggac gctgcgggtt agtaaccgat
    12001 tcggccatct ctgcagcggc tttgctactt aaccccatgg tggcggctaa tgctgcacaa
    12061 agcttcatga aatcacgacg gttaatgccg tgagaatgga tgagggtgtt atctccagtc
    12121 atttatagtt attccgttgc gaagacctgg catatatttt gcctcaatcg caaaatcaat
    12181 aatgcgatcg atgcgccatt taccacacat ttattatggt tatcgatatc atgatactgc
    12241 ggcggggcgc taaaacgaag ggaagaagca ttagtgtaat taattaaagc aagataatac
    12301 gtatgtttga tcaattttcg aggcgataac cggccataaa aaagcgcaac gtagaacagg
    12361 aattattctc acgaaagaaa tccattacca tctctttatc cagtccatag cggcgcgtca
    12421 gaatacccgc ttcccaggcg ctaagctggc gatctccggt cttttcgtac atccggtgtg
    12481 agtagcctaa tacaaaaccg cgtttataat cggcacagaa ttttgtcaca ttaaccgcac
    12541 tgtcggcatg cgttgctttt aatcccgcca ttaagccttt accaaaatgg ttattcatcg
    12601 ttcacctcat tcgctatata tctccatata tagcgatatt ttaggcaaag aacgactttt
    12661 gcgcacgcta ttttttcatg cttgtttaag acgtaatcca gtaagagata aacctatcag
    12721 gagagatatt tatcgaacca tttcagtgtc ctttgccagg caagatcggc ggcagatttg
    12781 tcataacggg gcgtggaatc attatggaat ccgtgattaa cccccggata gatatacgcc
    12841 tcataaacct tattattggc tttcaacgcc gcctcgtaag caggccagcc ctcgttgatt
    12901 cgggtgtcca gttccgcgaa gtggagtagt aaaggcgctt caatcttcgc cacatcggca
    12961 gtgggtgcct gacgaccata aaacggcacc gcgcaggcca gttccggata cgcgacagcc
    13021 gccgcgttcg atacgccacc gccatagcaa aatccggtaa tacccacttt gcctgccgct
    13081 tgcggatagc gttgcataaa ctcaattgcg gcaaagaaat cattcatcag tttggttgga
    13141 tcaacctgct gttgcagctc acgaccttta tcatcatttc ccggataacc tccaacggaa
    13201 cttaagccgt caggtgccag ggcgatatac cccgccttcg ccactcgccg tgccacatct
    13261 tcgatatacg gattcagtcc acgattctca tgcaccacta ccacggctgg cgttttgccg
    13321 ctcatttttg cgggtttcac cagataaccc cgtacctcgc cgtgaccatt tggcgaagga
    13381 tacgtgatgt actcagcaac aatctccggg tcggtaaact ctacctgagt cgccagcgca
    13441 taatttggct tgagcaaatc aaacaacgct aatgccgtca tcccgcccac cgcatacttc
    13501 gccgcaaggt tgaggaactc acgtttcgag attttcccgt gagcgtaata gtcgtagtaa
    13561 tccaacaact cttgtgggaa atctttggcg gttaaacgcg gcatcgttgc actcctcaac
    13621 tggtgttttt ttaagcaaag cataagcacg tgtttttgcc cagtttttcg tcactctgtg
    13681 agccagacta cgggatacgc gctggcgaat cgctaaacta gaaacattgt ttcgaaattg
    13741 aacggtggaa aggagaggtc atggtctggt tagcgaatcc cgaacgttac gggcagatgc
    13801 aataccgcta ttgcggaaaa agcggtttac gcctgcccgc gttatcgctc ggtttatggc
    13861 acaatttcgg tcacgttaac gcgctggaat cacagcgtgc gatcctgcgt aaagcgtttg
    13921 atttgggcat tacgcacttt gatttagcca acaattacgg gccgcctcca ggaagcgcag
    13981 aagagaactt tggtcgcctg ctgcgggagg attttgccgc ttatcgcgat gaactgatta
    14041 tctctaccaa ggctggctac gatatgtggc ccggccctta cggctctggc ggttcacgta
    14101 aatacctgct cgccagcctc gaccaaagcc tgaagcgtat ggggcttgag tatgtcgata
    14161 tcttttactc tcatcgcgtc gatgaaaata cgccgatgga agaaaccgcc tctgcgctgg
    14221 ctcatgcggt acaaagcggt aaggcgctgt atgtcgggat ctcctcttac tcgccagagc
    14281 ggacgcaaaa aatggtcgag ttgctgcacg agtggaaaat tccgctttta attcatcaac
    14341 cttcgtacaa tttactgaac cgctgggtgg ataaaagcgg cctgctggat accctgcaaa
    14401 ataacggcgt gggctgtatt gcctttactc ctctggctca gggattgctg accggaaaat
    14461 atctcaacgg cattccgcaa gattcacgga tgtatcgtga agggaataaa gttcgtggtc
    14521 tgacaccgaa aatgcttacc gaagccaacc tcaacagcct gcgcttattg aatgaaatgg
    14581 cacagcagcg tggacaatca atggcgcaaa tggcgttaag ctggttgttg aaagatgatc
    14641 gcgtgacgtc ggtattgatt ggtgccagcc gcgcggagca actagaggag aacgtgcagg
    14701 cgctgaataa tctgacattt agcaccgagg agctggcgca gattgatcag catatcgccg
    14761 atggcgagct gaatctgtgg caggcgtcgt ccgataaatg acctgttaat aacgggcgcg
    14821 cgaaccgcgc ccgaatcaga tcagtgatta tgacgagtca gtcggtcaag atagcccatc
    14881 acaaatgcag aaagcacaaa cgtcagatgg ataatgacgt accacatcag tttgttatcc
    14941 gggacatttt tcgcatccat aaagacgcgc agtaggtgaa tggaagaaat tgccacaatc
    15001 gacgctgcta ctttgttttt cagcgacgtt gcgtccattt tccccagcca gttcagcttc
    15061 tctttgttct cggagatatc cagctgtgag acgaaattct cataaccgga aaacatcacc
    15121 atcaccagca aaccgccaac cagcgtcata tccaccagcg acagcaacac gaggatcaaa
    15181 tctgattccg ccatcgagaa gatattcggc agtacgtgaa taatctcctg gaagaacttc
    15241 agcgccaggg caactaacgc cagcgaaagg ccaaagtaca cgggggcaag cagccagcga
    15301 gaagcataca ttgcattttc aagaaaacgt tccatacatt cctgtcgtgt agttaaaacg
    15361 ggcaacagta tatcgcaatt gagtaaacag cacgcaacag caaatcaaca tatacgttaa
    15421 acctttgtcg cataattctg aggtttaggg atcttcctgg ttcccttatc aacgcatcga
    15481 gagcgtctac ccttaaagga cgttgaacct gaagggagaa aaacgatgtc tcatctaaaa
    15541 gacccgacca cgcagtatta cactggtgaa tatcccaaac agaaacaacc gacgccaggc
    15601 atccaggcga agatgacacc ggtaccggat tgcggcgaga aaacctatgt tggtagcggt
    15661 cgcctgaaag atcgtaaagc actggtgaca gggggcgatt ccggaatagg tcgcgctgcc
    15721 gccatcgctt acgcgcgtga aggggctgac gtggcgatca gttatcttcc cgtggaagaa
    15781 gaagacgctc aggatgtgaa aaagatcatt gaagaatgcg gtcgcaaagc cgttctgctg
    15841 ccaggcgatt taagcgatga gaaattcgcc cgttcgctgg ttcacgaagc gcacaaggcg
    15901 ttaggcgggc tggatattat ggcgctggtc gccgggaaac aggttgccat tcctgatatt
    15961 gcagacctca ccagcgaaca gtttcaaaag acctttgcca ttaacgtttt cgcgctgttc
    16021 tggctaaccc aggaagcgat ccccctgcta ccgaaaggtg caagtatcat caccacttcg
    16081 tcaatccagg cataccagcc aagtccgcat ttactggact atgcggctac gaaggcggcg
    16141 attctgaact acagccgtgg cctggcaaaa caggtcgcgg agaaaggtat tcgggtgaat
    16201 attgtcgcgc caggccctat ctggacggca ctgcaaattt ctggcggaca aacgcaggat
    16261 aagatcccgc agtttggtca gcaaacgccg atgaaacgtg cggggcaacc ggcggaactg
    16321 gcccctgtat atgtttatct ggcaagtcag gagtcgagct acgtcaccgc agaagtgcac
    16381 ggtgtgtgcg gcggcgagca tttaggttaa aaaaatgccc ggttgtgaaa agcaaccggg
    16441 catcattgtg aattcactct tacctgatgt ggcgaaaacg ccttatccgc ctacaaacgc
    16501 atgaagattc aacatgttgc aatagatttt gtaggcctga taagcgtagc gcatcaggca
    16561 ttgagcacct aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa cgcacgaaga
    16621 ttcaacatgt tgcaatagat tttgtaggcc tgatgagcgt agcgcatcag gcaattctac
    16681 tttacttcgc tttggcggtt tcttcgccga ccagacctat cttcaggtaa cccgcctgat
    16741 gcagcgtatc cattaccttc atcaacgtct cgtaatcgac ggttttatcc gctcggaaga
    16801 agatggtggt gtctttcttg ccttcggtta acgcattcaa cgccgtaatc attgtttcat
    16861 cggtgaccgg atcgttaccg ataaacatcg agttgtctgc cttcaccgac agataaaccg
    16921 gtttttccgg gcgcggctgc ggcgtgctgg tagaagcagg caagttcacc ttcacatcta
    16981 ccgtcgctaa cggtgccgcc accataaaga taatcagcag aaccaacatc acgtcgataa
    17041 acggcgtcac gttgatatca tgcatttcgc cgttatcgtc gaggttttcg ttaagatgca
    17101 ttgccatcgg atattatcct gcgcgtaatt tttgtgcgac acgaaccgga tgcgcagcgg
    17161 cgctggcttc cagatccagg tcacggcttt gcagcaacaa tacctgcgct gcaacatcac
    17221 ccagcatcgc tttaaagccg ccaatctggc gtgcaaatac gttatagata acgaccgcag
    17281 gaatcgctgc cacgaggccg attgccgttg ctaacagagc ttctgcgata cccggcgcaa
    17341 cgactgccag gttagtggtc tgcgtttgcg cgataccaat aaagctgttc atgatgcccc
    17401 agaccgtacc aaacagacca acgaacggag aaatcgcgcc gatggttgcc agatagccgt
    17461 taccgcggcc catttgacga cccactgcgg cgacccgacg ctccagacgg aagctggtac
    17521 gttctttaat accttcgtta tcgtcgctgc cttctgacag ttccagctca ttctgcgctt
    17581 cattgagcaa atgcaggctt aagcttttgc taccaaaatc agcggcgata tcgttggcct
    17641 ggtttaagga acgcgcttca gccagcagtt gctgctcgcg cttaaggcga cgcttctgat
    17701 tgaagaactc tacgctctta ctgaagaaga ttgcccaggt gactacggag gccaaaataa
    17761 gcccaatcat cacgcactta acgacaatat cggcgtgctg atacataccc cagacggaaa
    17821 ggtccgtctg cattaaatta ttacccactg tgtatctcca ggacgcaagt cacaaaatct
    17881 gcgcataata atatcaaaac gacgtcgaat tgatagtcgt tctcattact atttgcatac
    17941 tgccgtacct ttgctttctt ttccttgcgt ttacgcagta aaaaagtcac cagcacgcca
    18001 tttgcgaaaa ttttctgctt tatgccaatt cttcaggatg cgcccgcgaa tattcatgct
    18061 agtttagaca tccagacgta taaaaatagg aatcccgaca tggcggacaa aaagcttgat
    18121 actcaactgg tgaatgcagg acgcagcaaa aaatacactc ttggcgcggt aaatagcgtg
    18181 attcagcgcg cttcttcgct ggtatttgag agtatggaag ccaaaaagca cgcgacgcgc
    18241 aatcgcgcca atggcgagtt gttctatgga cggcgcggaa cgttaaccca tttttcctta
    18301 caacaagcga tgtgtgaact ggaaggtggc gcaggctgcg cgctatttcc ctgcggggcg
    18361 gcagcggttg ctaattcgat tcttgctttt gtcgaacagg gcgatcatgt gttgatgacc
    18421 aacaccgcct atgaaccgag tcaggatttc tgtagcaaaa tcctcagcaa actgggcgta
    18481 acgacatcgt ggtttgatcc gctgattggt gccgatatcg ttaagcatct gcagccaaac
    18541 actaaaatcg tgtttctgga atcgccaggc tccatcacca tggaagtcca cgacgttccg
    18601 gcgattgttg ccgccgtacg cagtgtggtg ccggatgcca tcattatgat cgacaacacc
    18661 tgggcagccg gtgtgctgtt taaggcgctg gattttggca tcgatgtttc tattcaagcc
    18721 gccaccaaat atctggttgg gcattcagat gcgatgattg gcactgccgt gtgcaatgcc
    18781 cgttgctggg agcagctacg ggaaaacgcc tatctgatgg gccagatggt cgatgccgat
    18841 accgcctata taaccagccg tggcctgcgc acattaggtg tgcgtttgcg tcaacatcat
    18901 gaaagcagtc tgaaagtggc tgaatggctg gcagaacatc cgcaagtagc gcgagttaac
    18961 caccctgctc tgcctggcag taaaggacac gaattctgga aacgagactt tacaggtagc
    19021 agcgggctat tttcctttgt gcttaagaaa aaactcagta atgaagagca ggcgaactat
    19081 ctggataact tcagtttatt cagcatggcc tactcgtggg gcgggtatga atcgttgatc
    19141 ctggcgaatc aaccagaaca tatcgccgcc attcgcccac aaggcgagat cgattttagc
    19201 gggaccttga ttcgcctgca tattggtctg gaagatgtcg acgatctgat tgccgatctg
    19261 gacgccggtt ttgcgcgaat tgtataacat tgccactttt ggacaatttt gcagacattt
    19321 aattgtgaaa agtcttaaat tgttgcgtcc gggatcaagg cgtcccggac gaatcaggag
    19381 tacaataggc agataaaggc ttaaacgctg ttccacagga aagtccatgg ctgttattca
    19441 agatatcatc gctgcgctct ggcaacacga ctttgccgcg ctggcggatc ctcatattgt
    19501 tagcgttgtt tactttgtca tgtttgccac gctgttttta gaaaacggcc tgctgcccgc
    19561 ctcatttttg ccaggcgaca gcttgttgat actggcaggc gcattgattg cccaaggggt
    19621 tatggatttt ctgcctacga ttgcgattct gaccgccgca gcaagtctgg gctgctggct
    19681 aagttatatt caggggcgct ggttagggaa taccaaaacg gtgaaaggct ggctggcaca
    19741 gcttcctgct aaatatcacc agcgcgccac ctgcatgttt gaccgccacg gtctgctggc
    19801 gctgctggct ggacgttttc ttgcatttgt ccgtacgctg ctgccaacca tggcgggaat
    19861 ttccggtctg ccaaaccgcc gcttccagtt tttcaactgg ttaagtggat tgctgtgggt
    19921 cagcgtggta accagttttg gctatgcctt aagtatgatt ccgttcgtta aacgccatga
    19981 agatcaggta atgacgttcc tgatgatcct gccaattgcc ttgttaaccg ctggcttgtt
    20041 aggcacgctg tttgtggtga ttaaaaaaaa atactgtaac gcctgacgat tttccccgtt
    20101 cccggctgct gtaccgggaa cgtatttaat tcccctgcat cgcccgcatt cttgccgcat
    20161 cttcccccgg cgtcacaccg aagtaacgtt taaactcacg gctaaattgc gatgcgcttt
    20221 catagccgac gcgcatcgct gctgcgctgg ccttcatgcc gtcatggatg atcatcatcc
    20281 gcgccttatg cagacggtaa ttcttcaaat actgcaacgg cgaggtgctg gtgacagact
    20341 taaaattatg gtggaacgcc gatacgctca tgttggcttc tgccgccagt tgctcgacgc
    20401 tcaggttttc ggtgtattta ttctcaatcc gtttcagcac gcggctaatc agactgaagt
    20461 gagtctggcg actgaccagc gccagtaacg cgccgccgca aggtccggtc agcacgtagt
    20521 acagaatttc gcggatgatc tgtttgccga gaatacgcgc atccagtggt cgctccatca
    20581 cgtcgagtaa ccgctccgcc gcgcataaaa tctcttctga taacgtggcg gagttaatcc
    20641 cgctggctgc catcgacggc tggaaatgct catcttcgcc aatgtccatc aacagttcct
    20701 gtaactgcaa aatatcgaca ttgagacgca accctgccag cggcacctct gacgtggcat
    20761 aggtttcgca ctcaaacggc aacggcaccg tcagcagcag gtattcattg gcatcataac
    20821 gaaacacgcg ttcattgata taaccgattt tatgcccgga aaagagaatt atgatgccag
    20881 gctcgtacat caccggtgta cgtgcgaaag gcgtctcgcc atacaacaaa cgcacatcgg
    20941 gcaacagttc tgacaaacta ttttctttaa ttttcagttt attaacttta tccgccagca
    21001 agcggcaaat ctcttcacgt ttcatatcgc gtaatttctt aggaataatg cggcaatttg
    21061 attgtgcgca attttgtagc atttctccag cactctggag gaataggcaa gacattggca
    21121 gaaatgagca ttgagagcca gggcgctggc gatcacaatg aaaaacatca ggcagatcgt
    21181 tctctgccct catattggcc cagcaaaggg agcaagtaat gaacaacttt aatctgcaca
    21241 ccccaacccg cattctgttt ggtaaaggcg caatcgctgg tttacgcgaa caaattcctc
    21301 acgatgctcg cgtattgatt acctacggtg gcggcagcgt gaaaaaaacc ggcgttctcg
    21361 atcaagttct ggatgccctg aaaggcatgg acgtgctgga atttggcggt attgagccaa
    21421 acccggctta tgaaacgctg atgaacgccg tgaaactggt tcgcgaacag aaagtgactt
    21481 tcctgctggc ggttggcggc ggttctgtac tggacggcac caaatttatc gccgcagcgg
    21541 ctaactatcc ggaaaatatc gatccgtggc acattctgca aacgggcggt aaagagatta
    21601 aaagcgccat cccgatgggc tgtgtgctga cgctgccagc aaccggttca gaatccaacg
    21661 caggcgcggt gatctcccgt aaaaccacag gcgacaagca ggcgttccat tctgcccatg
    21721 ttcagccggt atttgccgtg ctcgatccgg tttataccta caccctgccg ccgcgtcagg
    21781 tggctaacgg cgtagtggac gcctttgtac acaccgtgga acagtatgtt accaaaccgg
    21841 ttgatgccaa aattcaggac cgtttcgcag aaggcatttt gctgacgctg atcgaagatg
    21901 gtccgaaagc cctgaaagag ccagaaaact acgatgtgcg cgccaacgtc atgtgggggg
    21961 cgacgcaggc gctgaacggt ttgattggcg ctggcgtacc gcaggactgg gcaacgcata
    22021 tgctgggcca cgaactgact gcgatgcacg gtctggatca cgcgcaaaca ctggctatcg
    22081 tcctgcctgc actgtggaat gaaaaacgcg agaccaagcg cgctaagctg ctgcaatatg
    22141 ctgaacgcgt ctggaacatc actgaaggtt ccgatgatga gcgtattgac gccgcgattg
    22201 ccgcaacccg caatttcttt gagcaattag gcgtgccgac ccacctctcc gactacggtc
    22261 tggacggcag ctccatcccg gctttgctga aaaaactgga agagcacggc atgacccaac
    22321 tgggcgaaaa tcatgacatt acgttggatg tcagccgccg tatatacgaa gccgcccgct
    22381 aagcttttta cgcctcaaac tttcgttttc gggcatttcg tccagactta agttcacaac
    22441 acctcaccgg agcctgctcc ggtgagttca tataaaggag gaacgtatgg ctaatccaac
    22501 cgttattaag ctacaggatg gcaatgtcat gccccagctg ggactgggcg tctggcaagc
    22561 aagtaatgag gaagtaatca ccgccattca aaaagcgtta gaagtgggtt atcgctcgat
    22621 tgataccgcc gcggcctaca agaacgaaga aggtgtcggc aaagccctga aaaatgcctc
    22681 agtcaacaga gaagaactgt tcatcaccac taagctgtgg aacgacgacc acaagcgccc
    22741 ccgcgaagcc ctgctcgaca gcctgaaaaa actccagctt gattatatcg acctctactt
    22801 aatgcactgg cccgttcccg ctatcgacca ttatgtcgaa gcatggaaag gcatgatcga
    22861 attgcaaaaa gagggattaa tcaaaagcat cggcgtgtgc aacttccaga tccatcacct
    22921 gcaacgcctg attgatgaaa ctggcgtgac gcctgtgata aaccagatcg aacttcatcc
    22981 gctgatgcaa caacgccagc tacacgcctg gaacgcgaca cacaaaatcc agaccgaatc
    23041 ctggagccca ttagcgcaag gagggaaagg cgttttcgat cagaaagtca ttcgcgatct
    23101 ggcagataaa tacggcaaaa ccccggcgca gattgttatc cgctggcatc tggatagcgg
    23161 cctggtggtg atcccgaaat cggtcacacc ttcacgtatt gccgaaaact ttgatgtctg
    23221 ggatttccgt ctcgacaaag acgaactcgg cgaaattgca aaactcgatc agggcaagcg
    23281 tctcggtccc gatcctgacc agttcggcgg ctaacatgca aattctcccg gtggcggtaa
    23341 tgttccgcta ccggactttt cagaaatcat ttattcccct cgcgtcccgc ccgttgttac
    23401 tcttccttgt tcaggaatgc caaatataag gacatcatca tgcagagccg gaagctctta
    23461 aaagaacaac tcatctatat ccgggataaa cgcatcggag aggtgaaaaa cagatgaaaa
    23521 taatacttct gtttttagca gccctggcaa gttttaccgt acacgcacag cccccctcac
    23581 tgaccgtaga acaaacagtc cggcatattt atcagaacta taaatcagat gccactgccc
    23641 cttattttgg tgaaaccgga gagcgggcga taacttctgc gcgtattcaa caggcgctta
    23701 ccctgaacga caatcttacg ctgccgggca atattggctg gc
//
LOCUS       DAJOWB010000069        23232 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJOWB010000069 DAJOWB010000000
VERSION     DAJOWB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.069"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(79..423)
                     /locus_tag="OHO85_002915"
     CDS             complement(79..423)
                     /locus_tag="OHO85_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCQ2672262.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            555..2465
                     /locus_tag="OHO85_002916"
     CDS             555..2465
                     /locus_tag="OHO85_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCQ2672263.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGGDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2523..3218
                     /gene="tsaB"
                     /locus_tag="OHO85_002917"
     CDS             2523..3218
                     /gene="tsaB"
                     /locus_tag="OHO85_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCQ2672264.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3258..3839
                     /gene="yeaY"
                     /locus_tag="OHO85_002918"
     CDS             3258..3839
                     /gene="yeaY"
                     /locus_tag="OHO85_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCQ2672265.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADMN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4044..5729
                     /gene="fadD"
                     /locus_tag="OHO85_002919"
     CDS             4044..5729
                     /gene="fadD"
                     /locus_tag="OHO85_002919"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCQ2672266.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            5811..6926
                     /gene="rnd"
                     /locus_tag="OHO85_002920"
     CDS             5811..6926
                     /gene="rnd"
                     /locus_tag="OHO85_002920"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCQ2672267.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKVFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(6980..7945)
                     /gene="yeaX"
                     /locus_tag="OHO85_002921"
     CDS             complement(6980..7945)
                     /gene="yeaX"
                     /locus_tag="OHO85_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCQ2672268.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNHYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8001..9125)
                     /gene="yeaW"
                     /locus_tag="OHO85_002922"
     CDS             complement(8001..9125)
                     /gene="yeaW"
                     /locus_tag="OHO85_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCQ2672269.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVQDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            complement(9157..10767)
                     /gene="yeaV"
                     /locus_tag="OHO85_002923"
     CDS             complement(9157..10767)
                     /gene="yeaV"
                     /locus_tag="OHO85_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HCQ2672270.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            complement(11018..12103)
                     /gene="dmlA"
                     /locus_tag="OHO85_002924"
     CDS             complement(11018..12103)
                     /gene="dmlA"
                     /locus_tag="OHO85_002924"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCQ2672271.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            12206..13129
                     /gene="dmlR"
                     /locus_tag="OHO85_002925"
     CDS             12206..13129
                     /gene="dmlR"
                     /locus_tag="OHO85_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HCQ2672272.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            13256..13894
                     /gene="leuE"
                     /locus_tag="OHO85_002926"
     CDS             13256..13894
                     /gene="leuE"
                     /locus_tag="OHO85_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HCQ2672273.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            14067..14426
                     /gene="yeaR"
                     /locus_tag="OHO85_002927"
     CDS             14067..14426
                     /gene="yeaR"
                     /locus_tag="OHO85_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HCQ2672274.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYFGYPDEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            14430..14612
                     /locus_tag="OHO85_002928"
     CDS             14430..14612
                     /locus_tag="OHO85_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCQ2672275.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            14760..15008
                     /locus_tag="OHO85_002929"
     CDS             14760..15008
                     /locus_tag="OHO85_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCQ2672276.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(15067..15141)
                     /gene="yoaJ"
                     /locus_tag="OHO85_002930"
     CDS             complement(15067..15141)
                     /gene="yoaJ"
                     /locus_tag="OHO85_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCQ2672277.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(15144..15242)
                     /locus_tag="OHO85_002931"
     CDS             complement(15144..15242)
                     /locus_tag="OHO85_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCQ2672278.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            complement(15275..16300)
                     /gene="dgcP"
                     /locus_tag="OHO85_002932"
     CDS             complement(15275..16300)
                     /gene="dgcP"
                     /locus_tag="OHO85_002932"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCQ2672279.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            16483..16737
                     /gene="yoaF"
                     /locus_tag="OHO85_002933"
     CDS             16483..16737
                     /gene="yoaF"
                     /locus_tag="OHO85_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCQ2672280.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            complement(16759..17106)
                     /locus_tag="OHO85_002934"
     CDS             complement(16759..17106)
                     /locus_tag="OHO85_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCQ2672281.1"
                     /translation="MNIQCKRVYDPAEQSDGYRILVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(17161..18342)
                     /gene="nimT"
                     /locus_tag="OHO85_002935"
     CDS             complement(17161..18342)
                     /gene="nimT"
                     /locus_tag="OHO85_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCQ2672282.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     FNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            18439..19260
                     /gene="nimR"
                     /locus_tag="OHO85_002936"
     CDS             18439..19260
                     /gene="nimR"
                     /locus_tag="OHO85_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCQ2672283.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTHRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            complement(19217..19663)
                     /locus_tag="OHO85_002937"
     CDS             complement(19217..19663)
                     /locus_tag="OHO85_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCQ2672284.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            19833..19937
                     /locus_tag="OHO85_002938"
     CDS             19833..19937
                     /locus_tag="OHO85_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672285.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            complement(19938..20441)
                     /gene="yeaK"
                     /locus_tag="OHO85_002939"
     CDS             complement(19938..20441)
                     /gene="yeaK"
                     /locus_tag="OHO85_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCQ2672286.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(20484..21974)
                     /locus_tag="OHO85_002940"
     CDS             complement(20484..21974)
                     /locus_tag="OHO85_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCQ2672287.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     CLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILIDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            complement(22155..>23232)
                     /locus_tag="OHO85_002941"
     CDS             complement(22155..>23232)
                     /locus_tag="OHO85_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCQ2672288.1"
                     /translation="YLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSLA
                     LFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHTA
                     TWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNYT
                     IWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLLS
                     RPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEVF
                     GLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISLV
                     MTEADKALREAKSEGGNKVIIHHI"
BASE COUNT         5965 a         5638 c         5932 g         5697 t
ORIGIN      
        1 taatctctgg tcgatttatg taaaaccgac cagtattatc gataaattat cgtctgactt
       61 attttgcttc gataaagctt acaccgcagc cacaatctta atttcaactt tatacttcgg
      121 gttcatcaaa cccgcttgta ccgtgcagcg caccggcgca tgacccgcga caacccaggc
      181 atcccacgct ttattcatcg ccgcgaagtc gtttttatcg gccaggaaaa tggtggcatc
      241 cagaatgctc gatttattgc tgccctgttt ttccagcacg gcatcaatct gcgccagcgt
      301 gttggcggtt tgctcaaagg catcggcgtc gaggttttcc ggtacaccag tgtagtagag
      361 cgtgttgttg tggattacca catcagacca gcgggcttca gcatcgatac gaacgatagt
      421 catcattttg tcctcattat ccttccattt tcgtgctgca aagactgcca caatgtgagg
      481 tcgccgtcac cacttcaact ggcgaaagcg tcctcatcct gacataatcc ctgttcaaat
      541 caacaggggg tagtgtgacg gacgattttg caccagacgg tcagctggcg aaagcgatac
      601 caggctttaa gccgcgagaa ccacagcgac agatggcggt agccgtcacc caggcgatag
      661 aaaaaggcca gccgctggtg gtggaagcag gaaccggtac gggcaaaacc tacgcttacc
      721 tggctcctgc gctgcgggcg aaaaagaaag tcattatctc gaccggctca aaagcgttgc
      781 aggatcagct ctacagccgc gatttgccaa cagtctcaaa ggcattgaaa tatacgggca
      841 acgtggcgct gctgaaaggg cgctcaaact acctctgcct cgagcgtctc gaacagcagg
      901 cgctggcggg gggcgatctg ccggtacaaa tcttaagcga tgtgatcctg ctgcgctcct
      961 ggtctaatca aacagtcgat ggtgatatca gcacctgcgt cagcgtggcg gaagattcac
     1021 aggcgtggcc gctggtcacc agcaccaacg acaactgtct tggcagcgac tgcccgatgt
     1081 ataaagattg ctttgtggtc aaagcacgta aaaaagcgat ggacgccgat gtggtggtgg
     1141 taaaccatca tctctttctg gcggatatgg tggttaaaga gagtggattt ggcgaactga
     1201 tcccggaagc ggacgtcatg atcttcgacg aagcccacca gctaccggac attgccagcc
     1261 agtattttgg tcagtcactc tccagtcgac aactgctcga cctggcaaaa gacatcacca
     1321 tcgcttaccg caccgaatta aaagacaccc agcagttaca aaagtgcgct gatcgtcttg
     1381 cccagagtgc gcaggatttt cgtctgcaac tcggtgagcc aggttatcgc ggtaacctgc
     1441 gtgagctgtt agctaatccg caaattcagc gggcattttt actgctcgat gacaccctgg
     1501 aactttgtta tgacgtggcg aaactgtcac tggggcgttc cgccttgctg gatgcggcat
     1561 ttgagcgcgc tacgttgtat cgcacacggc tgaagcggct aaaagagatc aatcagccgg
     1621 gctacagcta ctggtacgaa tgcacttcgc gccattttac tctggctctc acgccgctca
     1681 gcgtggcgga taaattcaaa gagttaatgg cgcaaaagcc tggtagctgg atcttcacct
     1741 cagcaacgct gtcggtgaac gacgatctgc atcatttcac ctcgcggctt gggatagaac
     1801 aggcggagtc gttgctattg ccaagcccgt ttgattacag ccgccaggcg ttactctgtg
     1861 tgccgcgcaa tctgccgcaa accaaccagc caggttctgc tcgccagtta gcggcaatgc
     1921 tgcgaccgat catcgaagct aacaacggtc gttgttttat gctttgtacc tcgcacgcca
     1981 tgatgcgtga tctggccgag cagttccgcg ctaccatgac gcttcccgtt ttgttgcagg
     2041 gggaaaccag caaagggcaa ctgttgcagc aatttgtcag cgccggtaac gcgcttcttg
     2101 tggcaaccag tagtttctgg gaaggggggg acgtgcgtgg cgatacattg tcattggtga
     2161 ttatcgacaa gttgccgttt acctcgccgg atgatccact gttaaaagcg cgcatggaag
     2221 attgtcgttt gcgcggtggc gacccgttcg atgaagtgca actaccagat gccgtcatta
     2281 ctctcaaaca gggggtaggg cgactgattc gcgacgccga cgatcgtggc gtgctggtga
     2341 tttgtgacaa tcggctggtg atgcgtcctt acggcgcgac gtttctcgcc agtctgccgc
     2401 ccgcgccacg cactcgtgac attgcccgtg cggttcgttt ccttgcgata ccatcctcca
     2461 ggtaatttgt tacggagtat ggtatgatgc gcgccttttt attgatctaa gcacgaagac
     2521 ccatgcgaat tctggctatc gataccgcga cagaggcctg ctctgtcgcc ctgtggaacg
     2581 acggtactgt caacgctcat tttgaacttt gccctcgtga acatactcaa cgaatcttac
     2641 cgatggtgca ggatatcctg accaccagcg gaacttccct gactgatatt aacgctctgg
     2701 cttacgggcg cggccccggt agctttaccg gcgtgcgcat tggtattggc attgcgcaag
     2761 ggctggcact tggcgcagag ctaccgatga ttggcgtctc cacgctaatg acgatggcgc
     2821 aaggggcgtg gcgcaaaaac ggcgcaaccc gcgtgctggc agccattgac gcgcgaatgg
     2881 gcgaagttta ctgggccgaa tatcagcgtg atgaaaacgg tatctggcgc ggtgaagaaa
     2941 ccgaagccgt actcaaaccc gaactcgttc atgaacgaat gcaacagctt tccggcgaat
     3001 gggtaacggt agggacgggc tggcaagcct ggccggatct cggtaaagag agcgggctgg
     3061 ttttgcgcga cggagaagtt ttactgccag cggcggaaga tatgctgccg attgcgtgtc
     3121 agatgtttgc cgagggtaaa acggtggcgg tggaacatgc cgaaccggtt tatttacgta
     3181 acaacgtcgc atggaagaaa cttccgggca aagaatgaat cttaagagta tgccctgaga
     3241 aaaaaaggag tcgcatcatg gcggttcaaa agaatgttat caaaggcata ctggcaggta
     3301 cgtttgcgct aatgctgagc ggttgtgtca ctgtgccgga cgccattaaa ggcagcagtc
     3361 ccacgccgca acaagattta gttcgggtga tgagtgcgcc gcagctgtac gttggtcagg
     3421 aggcacgctt tggtggcaaa gtggttgcgg tacaaaacca gcaagggaaa acccgcctgg
     3481 aaattgctac cgtaccgctg gacagcggag caagaccgac gctgggagaa ccttctcgcg
     3541 gtcgcattta tgccgatatg aacggttttc tggacccggt ggatttccgt ggacaactgg
     3601 ttacggtagt cgggccaatc actggtgcgg ttgacggaaa aatcggcaac acgccctata
     3661 aatttatggt gatgcaagta acgggttaca aacgttggca tttaacccag caggtgatta
     3721 tgccgcctca gccgattgat ccatggtttt atggcggtcg tggctggccc tatggctatg
     3781 gcggatgggg ctggtataat cccggccccg cgagagtaca aacagttgta actgaataat
     3841 tgcttgtttt taaagaaaaa gaaacagcgg ctggtccgct gtttctgcat tcttacggta
     3901 aagataaaaa taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt atcaagctgg
     3961 tatgatgagt taatattatg ttaacggcat gtatatcatt tggggttgcg atgacgacga
     4021 acacgcattt tagaggtgaa gaattgaaga aggtttggct taaccgttat cccgcggacg
     4081 ttccgacgga gatcaaccct gaccgttatc aatctctggt agatatgttt gagcagtcgg
     4141 tcgcgcgcta cgccgatcag cctgcgtttg tgaatatggg ggaggtaatg accttccgca
     4201 agctggaaga acgcagtcgc gcgtttgccg cttatttgca acaagggttg gggctgaaga
     4261 aaggcgatcg cgttgcgttg atgatgccta atttattgca atatccggtg gcgctgtttg
     4321 gcattttgcg tgccgggatg atcgtcgtaa acgttaaccc gttgtatacc ccgcgtgagc
     4381 ttgagcatca gcttaacgat agcggcgcat cggcgattgt tatcgtgtct aactttgctc
     4441 acacactgga aaaagtggtt gataaaaccg ccgttcagca cgtaattctg acccgtatgg
     4501 gcgatcagct atctacggca aaaggcacgg tagtcaattt cgttgttaaa tacatcaagc
     4561 gtttggtgcc gaaataccat ctgccagatg ccatttcatt tcgtagcgca ctgcataacg
     4621 gctaccggat gcagtacgtc aaacccgaac tggtgccgga agatttagcc ttcctgcaat
     4681 acaccggcgg caccacaggt gtggcgaaag gcgcgatgct gactcaccgc aatatgctgg
     4741 cgaacctgga acaggtcaac gccacctatg ggccgttgct gcatccaggt aaagaactgg
     4801 tagtaacggc actgccgctg tatcacattt ttgccctgac cattaactgc ctgctgttta
     4861 tcgaactggg tgggcagaac ctgcttatca ctaacccgcg cgatattcca gggttggtaa
     4921 aagagttagc gaaatatccg tttaccgcta tcacgggcgt taacaccttg ttcaatgcgt
     4981 tgctgaacaa taaagagttc cagcagctgg atttctccag tctgcatctt tccgcaggcg
     5041 gagggatgcc agtgcagcaa gtggtggcag agcgttgggt gaaactgaca ggacagtatc
     5101 tgctggaagg ctatggcctt accgagtgtg cgccgctggt cagcgttaac ccatatgata
     5161 ttgattatca tagtggtagc atcggtttgc cggtgccgtc gacggaagcc aaactggtgg
     5221 atgatgatga taatgaagta ccaccgggtc aaccgggtga gctttgtgtc aaaggaccgc
     5281 aggtgatgct gggttactgg cagcgtccgg atgctacaga tgagatcatc aaaaatggct
     5341 ggttacacac cggcgacatc gcggtgatgg atgaagaagg gttcctgcgc attgtcgatc
     5401 gtaaaaaaga catgattctg gtttccggtt ttaacgtcta tcccaacgag attgaagatg
     5461 tcgtcatgca gcatcctggc gtacaggaag tcgcggctgt tggcgtacct tccggctcca
     5521 gtggtgaagc ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc gaagagtcac
     5581 tggtgacctt ttgccgccgt cagctcacgg gctacaaagt accgaagctg gtggagtttc
     5641 gtgatgagtt accgaaatct aacgtcggaa aaattttgcg acgagaatta cgtgacgaag
     5701 cgcgcggcaa agtggacaat aaagcctgag tgttaagtca gtcgtcagac gccggttaat
     5761 ccggcgtttt ttttgacgcc cactaaagag aaaacaattt gaattaccaa atgattacca
     5821 cggacgatgc gctggcttct ttgtgtgaag ccgtccgtgc ctttccggcg atagccctgg
     5881 atactgaatt tgttcgtacg cgcacttatt acccgcagct ggggttgatt caacttttcg
     5941 atggcgagca tctggcgcta attgatcctc tcgggatcac cgactggtca ccgctgaaag
     6001 cgatcctgcg cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa gatctggaag
     6061 tgttcctcaa tgtctttggc gaattaccac aacccttgat tgacacgcaa atccttgctg
     6121 ccttctgcgg acgcccgatg tcatggggtt tcgcttccat ggtggaagag tattccggcg
     6181 ttacgctgga caagagtgaa tcgcgcaccg actggctggc cagaccgctg accgaacgtc
     6241 agtgtgaata cgcagcggcg gatgtctggt atctgttacc gatcaccgcc aagcttatgg
     6301 tagaaacgga ggcctccggc tggctacctg cggcgctgga tgaatgccgc ctgatgcaaa
     6361 tgcgtcgtca ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc aatgcctggc
     6421 agttacgcac acgccaactg gcctgtctgc aactgttagc cgactggcga ctgcgcaagg
     6481 cgcgagagcg tgatctggcg gtgaactttg tcgtgcgtga agagcatttg tggtcggtcg
     6541 cgcgttatat gccgggaagt ttaggcgaac tggacagcct gggtttatcc ggtagcgaaa
     6601 tccgctttca cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca ttgccggaag
     6661 aggccttacc gcagccgatg cttaacctga tggacatgcc tggttatcgt aaagtgttta
     6721 aagcgattaa gtcgctgatt actgacgtga gcgaaacgca taagatcagc gccgaattgc
     6781 tggcatcgcg tcggcaaatc aaccaactgc tgaactggca ctggaaactg aaaccgcaga
     6841 acaatttgcc ggagctgatt tccggctggc gtggtgagct gatggcggaa gcattacaca
     6901 atttattgca ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg cgctcagcgc
     6961 cacatccggc tttggcaaac tacaaatcca acaccaggcg tttacccttc gcacgcgaac
     7021 aacagatcaa catactttgc tggctggcac gctcttcatc gctaaaatac tgatcccgat
     7081 ggtcagcttc accttccagt attgctgttt cgcaggttcc gcatacccct tcacgacata
     7141 aacattccac tttcgcggct ttattatttt caataacctg caaaatagtc atctcttccg
     7201 gcaccacaaa ctcttttccg gaacgggcaa gcaccagggt aaaggcatcg ccggttttgt
     7261 cttcgatagc aaattgctca aagtgcagcg tatcggcgac gatgtccaga cgcgcagctt
     7321 cacttcttac cgcttcattt agcgcctcgg gaccacaggt ataaacgtgt gtgccaggtt
     7381 cgatatccgc caataatcgc gctaattcca gtcgtgttcc ggttgatgag tgatgcaagt
     7441 gaactttctc agcctgcgga tgctggacta gctcatcacg aaatgcgcaa ctttctggat
     7501 ttcgcgagca gtaatgtagc tgccagtcga cgtcgctgtg ttgcagctct gccatgtgcg
     7561 acaggaaagg ggtgatacca ataccgcccg cgataaacag atgctttctg gctgagggaa
     7621 ttagcgcaaa caggttatta ggcgttgaaa tcgttaaccg atcgcccact tttacctgct
     7681 gatgcaaaaa gcgggaaccg ccgcgcgagt tttcctccag ccgaacggca atctgataac
     7741 aagaggtgtc atgcggcgaa ctcagtagtg aatacgcatt gctgtaatgg ttatcaccat
     7801 cgctcatctg cacaatgatg tgacttcctc cggtgaacgc aggcagcggt ttgccatcgg
     7861 ttgccaccag cgtgaagcgt ttcacctgtt cggtaagcgg ttccacctga ctcacctgta
     7921 cttcaaacat ttgatagtct gacattgctg tactccatca aaaatcagcc cagcggcgga
     7981 aaataccgcc gccgatgtca ttagtcctta aacacctgcg ccagcagatt atggaaatgg
     8041 gcgataccat gttcggaaat gccactaccg ctactgtcgg ccatgatgcg cccctgacca
     8101 cgatagccac gcgatttcag ccctttctgt acgctttcaa ccagacgtaa atcttccgga
     8161 cggaacacat cgcgatacca ctcaatcagc gatttttgct cgtcggttaa ctcttcattg
     8221 gtgaagtaaa tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa ttcataaatg
     8281 accgtcatca tccctttgat cggggtgacg ttcagcatcg tgcacggcca cagccagaaa
     8341 ccgtggaatg ccgcatccgt accctcttca aatttaaacg actgttcgga cggtttggcg
     8401 aaaccgtatt gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc aacctgtacg
     8461 gagtcggaga aacctggatg cgccggacca cagtgatagc actcgagata gttatcgaca
     8521 atgttcttcc agttggcagg cgtgcgggtg gtaaagcggg ccgccagttt cagatcttgg
     8581 acttccgggc aggcttccag cactttcgcg cccagaccgg gtaactgatc ttctacgctg
     8641 gtggcgttgg ggtccatgtt gatgaagacg aatccggcat attcttccag acgcaccgga
     8701 accagttgcg ctttgtcgct atcgaaattg gcgacgtttt cgcagttacg tgcatgggcc
     8761 agattgccat cgagtttgaa tgcccatgcg tgatacgggc aggtaatcac attttttgct
     8821 tttccttcac cgctcaacaa ctgatgacca cggtgcggac acacgttata gaacgcgcgc
     8881 aaaaccttat cacgaccgcg taccagcacg atgctttcgc caatgatctc acgcgtcaca
     8941 taatcattgg cattcgccag ttcgctgctg tgagcgacgc aaatccagct tttggcgaag
     9001 acgttctctt tttcgtgttc aaacgcgttc tgatcggtat aaaaacgggc aggaatggtc
     9061 cacgcctctt gcgggttagc gcaaaaattt tcgggtagta caaagtcagg gctcagattg
     9121 ctcataggga tacctttatc gttaggatga tggcgctcag ttgtcgcctt tgaatacggt
     9181 tcccgcaggg agcactggcg ttttttccag ggcttcaacc ggtgtctgcg ggagataatg
     9241 ttcaacttga tgagccggaa tgtcggcgta atcctgtttc agccagcgaa taaatccgcc
     9301 gactttcacc agtaaaatga cgaggaaggg aagggctgtg agcacgacgg tggttttcat
     9361 cgtttccagc gaagcaccgg taaacaagat ggaaagcggg atcagagtga tcaccacgca
     9421 ccagaaaaga cgcagcccac ggtcaggatc gtcaccttcc tggagattac gcgtactggt
     9481 cgcagccatg gtgtaggcca ccgcatccat atgcgaggca aggaaaataa tcatcacgcc
     9541 caggtatgcg gcgaggaaca atttaccggc tggcaacgac atcagaactt gctgtacagc
     9601 tgtttcgccg cccagtgttt ccagcacctg tgggacgttg attacgccat tgataaactg
     9661 atgaatggca tagctttcca ttacgccgaa gaagaaccag caaccgacgg tgctgccgag
     9721 gatcagtccc cagataactt ctttaatctt acgaccgcgg gaaacgcggg tgacaaacat
     9781 tgctacgcca ggggtgtatg agatccacca cagccagtag aaaacggtcc agttgcgggt
     9841 aaatgaacca tcgccaagcg gatcggtgaa taagctcatt tgcaggaagt tttgcgtggt
     9901 cagaccgatg gcattgatga tgctgttggt aataaattcg gttgggccga caatcagcac
     9961 cagtaatggc agcaggaacg cgccccagcc aaccattttg ctcagacgtt gcaaaccgtt
    10021 gttgatacca atccacgagc ttaggcaaaa aatgccgccg gaaagcagga tcacaaatgc
    10081 ctgcacggtg aagttatcgg gtaaaccggt cagcgcggaa agtccacgag taaaggttgc
    10141 tgcggtgaca acaagggaaa tagtcagtgc gccgacagtg gcgatcagga acatcaaatc
    10201 aaccagtttt ccccatgggc cttgcgggcg aacgccggta atcgcggcaa taatgccgga
    10261 aagactcaga cctttgtttt tacgcacatg aaagtgataa gccatgatta atgaggccag
    10321 cgtataagtt gcccaggcgc tgatgcccca gtggaagaag gagtaaggga cgctaaattc
    10381 gagtgcctgt tgtgaacgcg gtgcgatatt taatccaggc gtttgataat aataggccca
    10441 ttcagcaacc ccccaataaa gcgtagaaga acctaaaccg gcacaaataa acataaacag
    10501 ccaggagagc gtgctgtatt ccggttttcc ttcgccaaga cgaatattgc cgtatttact
    10561 ggtggctaaa taaatcacca gtcccattgc cagcaaaacc aatacctgta cggcggaacc
    10621 taacgtacgc gtaacaaaag tgtatattgc gttggccgcg tcggccgatt gttgtgggaa
    10681 aaggcttaat gcagctgcga tgaaaagaat ggccaccagg ctgatgctta tcagcggcac
    10741 atcttttttc ttaacattgc tcatcatgag tcatctcctg ggcataaaag cccgtctctg
    10801 gcttttgcca gttgtaatta tttgcagggt aatgagttgt atgttgtgtt taacaaattg
    10861 attatttgca tgttacataa attaaacaat taagaatacg gaaagccata aaacacgaaa
    10921 cggaaacaat aaaaggtgaa atgacgcagg ttatttactg caaagaacga ataaaaaaac
    10981 cgcgtcagtg acgcggttca aataaactgg tttgacctta acgcaaaata attttgcaaa
    11041 tcgcgtcggc aacctgtggc gtggtggcat tgcctttcat atcaggtgtt ttcggcccgt
    11101 gagcaatcac ttcttcaatt gctgccagaa taccgttatg cgcttgctgg aaacgctcat
    11161 cgccattgcc gagaaaatcg agcatcattg caccggccca aatcgtggcg atagggttag
    11221 caatgttttt cccgtagata tccggcgcgg aaccgtggac tggctcgaac agcgacggga
    11281 aagtgcgttc cggattcagg ttggcggatg gggcaatgcc aatggtgccg gtgcaggccg
    11341 ggccaagatc ggaaaggata tcgccaaata aattggacgc caccaccaca tcgaagcgtt
    11401 ccggctgcat cacaaaacgc gcgcagagaa tatcaatatg ctgcttgtcc cagcggatct
    11461 ccgggtaatt ctcggccatt gcttccactc gctcatccca gtacggcatg ctgatggcta
    11521 aaccgtttga tttagtggca gaagttagcg tcttacgtgg gcggctttgc gcaagttcga
    11581 aggcataacg caaaatgcga tcgacaccac gacgcgtaaa taccgattcc tgaatgacga
    11641 cttcatgctc tgtaccttca ttcactctac cgccgagcga ggaatattcg ccttcggtgt
    11701 tttccctgac cacgtaaaaa tcgatgtcgc caggctgttt tcccgccagc gggcagggaa
    11761 cgccaggaaa gagacgaacc gggcgcaggt tgacgtactg gtcgaattca cgacgaaatt
    11821 tcagcagcga accccacaac gaaatatggt caggaacggt gtccggccag ccgacggcac
    11881 caaaatagat ggcgtcgaaa cggctaagtt gctcatgcca gtcgtccggc atcattttac
    11941 cgtgatggct gtaatactcg cagctcgccc actccatttg ctcaaaactc aaggcgaagc
    12001 cccagcgctc agcggcagcc tgtaacacgc gaatcccttc aggaaggact tctttgccaa
    12061 tcccgtctcc cgggatcgca gcaatacgca tcgttttcat cattgggttc tcacttcctg
    12121 accatcaaaa ttgacaacat caccatccta taattgactg aaaaggaatt aatccccgta
    12181 actggtgaaa cataaaacac gaatcatgaa taatttaccg ctgctgaatg atttgcgcgt
    12241 ctttatgctg gtggctcgtc gggctggttt tgccgccgtg gcggaagaac tgggcgtttc
    12301 accggcgttc gtcagcaagc gcatcgcctt gctggagcaa acgctaaacg tggtgttgtt
    12361 acaccgcacc acccgccgcg tcaccattac cgaagaagga gagcggattt atgaatgggc
    12421 gcagcggatt ttgcaggatg tcggtcagat gatggacgaa ctttctgacg tgcgtcaggt
    12481 gccgcagggg atgctgcgta ttatcagcag ctttggtttt ggacgacagg tggtggctcc
    12541 ggcgttatca gcgctggcta aggcgtatcc gcaactggaa ttacgttttg atgtggaaga
    12601 ccggttggtc gatttggtca atgaaggtgt tgatctcgat attcgcattg gcgatgacat
    12661 cgcccctaat ttaatcgccc gcaaactggc gactaattac cgcattctct gcgcgtcgcc
    12721 ggaatttatt gcgcaacatg gcgcgccgaa acatttaacc gatctttccg cattgccgtg
    12781 cctggtcatt aaagagcgcg atcatccttt tggtgtctgg caattacgca ataaagaagg
    12841 ccaccatgcg attaaagtca ccggcccact gtcgtctaat catggagaaa ttgtccatca
    12901 gtggtgtctc gatgggcagg ggattgctct gcgctcctgg tgggatgtta gcgaaaacat
    12961 tgccagtggt catttagtgc aagtgctacc ggaatattac cagccagcga acgtctgggc
    13021 cgtttatgtt tcaaggctgg cgacgtcagc gaaagtgcgg ataacggtag aatttttacg
    13081 ccagtatttt gccgagcact acccgaattt ttcactggag catgcctgat taatgattca
    13141 attatcgggt tgatatcagg ttaaaacctg attttctcct ttctaagccg ctacagattg
    13201 gttagcatat tcacctttaa tcgcgcatga tcgaaagata attaaagagg ttaatgtgtt
    13261 cgctgaatac ggggttctga attactggac ctatctggtt ggggccattt ttattgtgtt
    13321 ggtgccaggg ccaaatactc tgtttgtact caaaaatagc gtcagtagcg gtatgaaagg
    13381 cggttatctt gcggcctgcg gtgtatttat tggcgatgcg gtattgatgt ttctggcatg
    13441 ggctggagtg gcgacattaa ttaagaccac cccgatatta ttcaacattg tacgttatct
    13501 tggtgcgttt tatttgctct atctggggag taaaattctt tacgcgaccc tgaagggtaa
    13561 aaatagcgag gccaaatccg atgagcccca atacggtgct atttttaaac gcgcgttaat
    13621 tttgagcctg actaatccga aagccatttt gttctatgtg tcgtttttcg tacagtttat
    13681 cgatgttaat gccccacata cgggaatttc attctttatt ctggcgacga cgctggaact
    13741 ggtgagtttc tgctatttga gcttcctgat tatttctggt gcttttgtca cgcagtacat
    13801 acgtaccaaa aagaaactgg ctaaagtggg aaactcactg attggtttga tgttcgtggg
    13861 tttcgctgcc cgactggcga cgctgcaatc ctgatacatt cagcccgcgt tgtcgcgggc
    13921 ttcccacttg taatcctccc tgatttttct ctgatatggt gctaaaaagt aaccaataaa
    13981 tggtatttaa aatgcaaatt atcaggcgta ccctgaaacg gctggaataa accgttttca
    14041 gcgcattcac cgaaggaggg aaaaggatgc tgcaaatccc acagaattat attcatacgc
    14101 gctcaacgcc tttctggaat aaacaaactg cgcctgccgg aatattcgaa cgtcatcttg
    14161 ataaaggaac gcgcccgggg gtttacccac gcctttccgt tatgcatggg gcggtcaaat
    14221 atttcggcta ccctgatgaa cacagtgcag agcctgatca ggtgatcctt atcgaagcgg
    14281 ggcagtttgc ggtgttccct ccagaaaagt ggcacaacat tgaagccatg actgacgata
    14341 cttatttcaa cattgacttc ttcgtggctc ctgaagtcct gatggaaggt gcgcaacaac
    14401 ggaaagtcat tcataacggg aaatgaatca tgggcaaagc aacgtatacc gtgaccgtca
    14461 ccaataacag caatggcgtt tctgtcgatt atgaaacaga gacgccgatg actttgctgg
    14521 tgccagaagt ggcggctgaa gtgataaaag atctggtgaa taccgtacgt tcttatgaca
    14581 cggaaaacga acatgatgtt tgtggttggt aattatcact gatcgttgat ggcgcaattt
    14641 ctgcgccatc gcaaaaaaaa cctccgtgat gctcgtcaca aatcttgccc tcccgatgtt
    14701 gtgctatgct ttttatcagc gactaacccg ctaatgcaaa acagataagt gaggaaacaa
    14761 tgggcattct gtcatggatt atttttgggc ttattgccgg tattctggcg aagtggatca
    14821 tgccaggtaa agatggcggt ggattcttta tgactatcct gctggggata gtcggtgccg
    14881 tagtcggcgg atggatcagc acgctgtttg gctttggtaa agtcgatggc ttcaattttg
    14941 gcagcttcgt ggttgccgtt attggtgcga ttgtcgtgct atttatctac aggaagatta
    15001 aaagttaacg cttaaattgc acaaaggctg cacacaggca gcctttgcta ttttttagag
    15061 gtgacgttac caccatccca gctcagtgcc gagtacgaca ataatcgcca cacccatcat
    15121 aataatcgtt gtttttttca tctttatgct cccggggcag catagccgcc aataaaaaac
    15181 catgctaaaa atacgaccgc tgtaatgaag attacaaccg gaaaaataat accgattcgc
    15241 atgttatcac caatcaaaag gatgtgaata cgcctcagga atgtagcgct ggatgcgcga
    15301 caaaaggtgt tttctgtttg tgttttttct cctgatacat cgcaatatcg gcagcatgca
    15361 gggcactgtc tgcatcggtt gtttcaggat cgacttctac tatgccaaga ctggcaccgg
    15421 gataatacaa atcaacatca cctaagtgat attctccacg tatttgctgc tgaatgcgtt
    15481 ctcgcagcga cgaaatatcc gcattctcgt tgtttagtga aacaactaaa aactcatcac
    15541 cgccgagacg accaataact tcgccatttt gctggagcgt attaaggcgc tcgccaacct
    15601 gaatgagaaa cagatcgcca ctattatgac caaaacgatc attgattaat ttgaagttat
    15661 ccagatcgat aaacgcgatc attatcttat ggttaagatg ccgggcgagg gaaaacagtg
    15721 tcgtcagatt ttcaaaaatc gcccgccgat tcggtagccc ggttaacgag tcggtatacg
    15781 attgcgcaat cagcgcagca ttggcttcgc gcagctgttc aaccagtgcc tctttttgaa
    15841 tatattgtgc aatcagtccg gcgaataact gtaaaacctg ttccgcgcgt tcactccact
    15901 ggcgcttctc actgctggcg gcgcaaagcg tgccatagaa tgatccatcc ggtaagtgaa
    15961 ttggcgtgct cagaaatgtg gtgatgccaa gattgcgtgc cgcaatacag tcaccccaac
    16021 ggtcggggac ttcatcgctg aaaaagcagt tttcatcaat ggcgcgtttg cataacgagt
    16081 aatcccacga gacggtaaaa ttctccggga tgtgcatttt ctggctgtta cgggcaaaca
    16141 taatatgctg caggcgcgct tcgacatcca ctttggtcag gtaggttgat tccatatcag
    16201 tgaccatttc cagcatctcc agaagctgtc ggaccagact ttccagtgac tgttctttgg
    16261 caagggattg cgagacgcgg gcgataatct gatctgacac ggaacaactc caggatacgg
    16321 cgggtaaatg ctgattattt aatctttgca ggcatgataa aacatcatca acaaagatga
    16381 aacaagacgt aatatgtttt gagtgaatgt aataaaaata gtgctgcaaa acaaaacgat
    16441 accgcgatac tctaatcttg gataaatatg tgagatcaat aaatgaaaat tatcagtttt
    16501 gttctgcctt gcttgctggt cctggctggt tgttcaaccc cttctcagcc agaagcacct
    16561 aaaccgccgc agattggtat ggcaaatccg gcgtcggtct attgccagca gaagggcggg
    16621 acgctcattc cagtgcagac agcgcaaggg gtcagcaaca attgcaaatt accgggcggt
    16681 gaaacgattg atgaatgggc attgtggcgg cgtgaccatc cggctggtga aaaataaccg
    16741 ccggatgctg tactaaaatc acaagctacg tagccagtcg gccagcacca gcgcatggtt
    16801 ctgcgtggtg ttttttgctg cgtagagcag ggtcagcggc tgttttttgg cgatgtccgc
    16861 cagccgcttt ccttcttgct cgtgttgtgc cagttctgca agatattgct cgcgaaaggt
    16921 tgcaaaatcg acgacttcgc cgtgaaaggc tttgcgcagt tccgttgacg gcgtgatttc
    16981 tttatcccac tcatcaaggg ctaaatcggt ttttttgata ccgcgcggcc agaggcggtc
    17041 gaccagtatg cgataaccat cgctctgttc tgccggatca taaacgcgtt tgcactgaat
    17101 attcatcgtg tttgttctcc ctttcacgca tgatttgtca cgtcactata gcaccggata
    17161 ttagcgaatt tctttgtctc ttccggcgca aagtccgaaa atcgccatca gtagtgaaag
    17221 tattgcaaca cccattagtg gtacggacca gtcaccgtta gcatcgtgta ttttacccat
    17281 cagcggcggc ccacaggctg ccagcaaata cccgacggat tgtgccatcc ccgagagtgc
    17341 cgccgcctga tgcgcagaac ttgcccgaag accaatgaac gtcaacccca gtatcattgt
    17401 tgcgccggaa ccaaaaccga aaagcagagt ccaggtgatc gcgtgtgctg gcataaagca
    17461 gagcccaacc gcgcccactg cgcacatcaa ggcaacgaac gctgcaatac cacgctgatc
    17521 tttcacatga tgtaagaaaa gtgggatcag caaaccggga gctgctgtgg ctagttgcag
    17581 caaaccatgc agtgaacccg cctgtgcttc gctatagccg tgactgatga ggatcgccgg
    17641 aagccagcca ataatcacgt aatagaccag tgagttgatc ccaagaaaca atgtgacctg
    17701 ccaggcaagc ggcgaacgcc agataccccg agtatgtaag gcgcgcgagg tactcaaatt
    17761 tgcatgttgt tggcttcgcc actgtggcag ccataaaaaa agagccagca gaggaaaaca
    17821 catcagcatg agcaacgcgc cttgccagcc aaaaccgtta aaagccagcg gcactaccat
    17881 agccgatcca agtgccgccg cagctcccat tgtcagggaa tatgcgccgg taagtctggc
    17941 gacggaatga gggaaatcgc gtttaattaa tcctggcagt aagacattac ctaatgcaat
    18001 cccaccacca atgaccgctg taccgccaaa taataagtaa ggcgaaggga gagagcgaat
    18061 tgcgatacca gcacagatca aaagtaacgc ggcaaacagg ctacgttcca taccaaaacg
    18121 tcgcgctacc ggggcggcca aaggtgagat tagcgcaaag gccaataatg gcagggtggt
    18181 caataagccg gtttgcgctg tcgtcagcga gtaagcggaa cgaatcgtat ccagtaacgg
    18241 tgctgcgccg gtaaaggtga cgcgtaatgt tgtggcaatc atcagaatgc cagcgataag
    18301 gacaatcctg tttttgccgc ttaatgaagt tgaacaggtc atatgcttct cattgttaca
    18361 aacgaggctg taccttaccg tttttattgt tatgttgaat caagctaaaa tgacaaatta
    18421 tcgctaaatt cggacaaaat gatgcacagg ttgaatctca atggctatga acccgaccga
    18481 caccatgaag ccgcggtcgc attttgtatt cacgccggaa cggatgagtt aaccagtcct
    18541 gtacatcagc accgcaaagg acagctgata ctggcgctac atggtgcaat tacctgtacg
    18601 gtggaaaatg ctttgtggat ggtgccgccg caatatgcgg tttggatacc gggcggggtg
    18661 gaacatagca atcaagtgac agctaacgct gaactctgtt ttctgtttat cgaacccagc
    18721 gcagtgacaa tgccgacaac atgttgcaca ttgaaaatat cgcccttatg ccgggaattg
    18781 attttaaccc tggcaaacag gacgacgact cagcgtgctg agccgatgac ccacagatta
    18841 attcaggtcc tgtttgatga actccctcag cagccacaac aacagctgca cctgcccgtt
    18901 tcttctcatc ctaaaatccg cacgatggtg gagatgatgg cgaaagggcc cgtcgagtgg
    18961 gggtcattag ggcaatgggc tggctttttt gcgatgagtg aacgcaatct tgcgcgactg
    19021 atagtaaaag agacggggtt gagttttcgc cagtggcgtc agcagttaca gctgattatg
    19081 gcacttcagg gattagtgaa aggcgatact gtacagaaag ttgctcatac gctggggtat
    19141 gattcaacaa cggcctttat tactatgttt aagaaaggcc tggggcaaac gccaggccga
    19201 tatattgcgg gattaactac tgtttcccca caatcagcga aaccagaccc gcggcaataa
    19261 gcgggccgac cggtacgccg cgaaacagcg ccacgcctaa aacagtgcct accagcagcc
    19321 cggcgaccag ttgcggctgg ctgcccatta aggtcacgcc gcgcccaccc agccaggaga
    19381 caatcacgcc tactgcaatt gccaccagcg atttccagtt cagaaacgag tgaatcaagg
    19441 ttgagggcgg tagcgtcccg ctggcgatgg gggccataac gccaatcgtc agaatgatta
    19501 tcccgatact tagcccctgt ttttcaatcc acgggaaaaa ggtgcttaac ggtgtgacgc
    19561 ggacaatgat caacaccaga attgaaacgg cgacagtggt gttatgactg ataaagccca
    19621 gcgcagctaa cccgagcagg atcagcaaag tgacatcaaa catcgtggtt tccttgccaa
    19681 aaaataataa gcctgcttac tttacgcttg ctgcaggaag gaagtagaaa atctttacgt
    19741 tttttgcata aaaaatcagg cgaactgtca tcaaactgcc atttgtgcac gctaacaata
    19801 acgtgaaaac agctatctcc cgggggcatg ctatgaacga tcaaatgttc gtcgagacac
    19861 tgattatcac gtcatcgttt tttgccattg ctgttgtgct ggttttgtcc gttctgctta
    19921 ttgaacggac aggctagtta cgcagtgcgg cggaaattga gcagttctgg ttgcgcaatg
    19981 cgcaaataat ccgtggtttt caaaataacg gatttatcca gcatccctgc attgaaggcg
    20041 atttcatcaa atcgttcgaa gagtagaggg tcggcaacca gtttgagttt tggatggaag
    20101 ctgaaagggg ggatcgcgcc gaagacacag cctgtgagtt catcgacttc cgccgggctg
    20161 gccaacgaag cgcgtaatcc gcctatatgg ctggcgagtt ggctcagatc ggcctgttga
    20221 tcggcagcga ggatcgccag aacatgttga tttacgccat tgcctttgac tttacatacc
    20281 agtgccttag ctccctggcc taaagcggtg ccacgaattt ctgacaccgc ttcacattta
    20341 cctaccgctt catgggtaac gacgcggaag ttagcacctt cttgtgataa taaagcgatt
    20401 aaccgctgat gcgtcacgct tcctttagcc atttcagtca tttcacgctc ctgagattac
    20461 aagcaaacaa ccacagaagg ttatcatgaa cggctgtttt tgttctgctt attgacatat
    20521 aaacgctcat cggaagcttt atacgcatca tgtaacgtat cgttttcttt catcgcgtaa
    20581 ataccggaag agaagccgat ctctttctgc ggcgcgatat gttgcagacg tttttcgata
    20641 cgttcaggca gttgtgctgc aatttgcggc gtcgaatcga taagaatgat gcagaattca
    20701 tcgccaccga gtcggatggc ataatcactt ttacgaatcg attgtttaat cgcctgagct
    20761 aacaacgtaa tcgctaaatc cccttcctga tgaccgaggg tgtcatttat tagctttaac
    20821 ttgtccatgt caattgcaat aaacatcacc gaagaaccgg attgcaccag tttctgcaac
    20881 cgctgctcca gttcaggggt taaaattttg cgattataca gtccagtcat cgcatcacta
    20941 atattttctc gcgacacatt ttggtataag cagaagtgca tccgcaccat attcagcaaa
    21001 attaacgccg tcaaaatcca gaacagaacg cttttccacg aagacgtgat aaagtaaaga
    21061 atatcaatgg ataacgagac acgaatgccg cccggtaagt catggacgta actgacatac
    21121 tggaacagat tatcttcgct ctggttgatg ataatgtcgc gccccgaatc ggtatcggtt
    21181 agcgtgacat tgagaaaacg ccagaggaga gggcggtcat gagtgtaaaa gatattccgc
    21241 aaattgtttt tgttaatatc cagcagcaca atccctttta gctgccctgc aacatacacc
    21301 ggcgtcagca aactcatcac gttctgttta gtacgctgat cctggtaaat gctcgacaat
    21361 acggtacgcc cggtaaatac cttttcaata tctttgcgat cgataccaat acttcctgac
    21421 tgcaggaagg accagttatt catcgcaaag tattcaacat taaccggctc ataaaaatag
    21481 acatagcggt cattgagatc gagataatag tgaaatttct cttcggccat cccaagacct
    21541 gaaccgtaat catcctggtg gcggttgcct tccactgcct gatcaaaagc gggtaatagt
    21601 gctgcagctt ccacaattgt gtcgcagtca gtggatgcgg tttgtagtgt gccgcgtaag
    21661 cccgggtatt tatgggcagt caggtttaag ccatgggtat tattggcaga ctcaaaagcc
    21721 tcgcagatgg cgcgccgctg ttctggcgac acttccgact gctcagcagc gagagcgcgc
    21781 atcacatgcg cggcaatact ctgattctga tacttatcat aaagaaagga ggagtctgct
    21841 ttctgcacga tatagcgcag ataggcactc attgctctgt cgctggcgac aagttcaaaa
    21901 ataaggaagg aagatgtcag cacaatgaca cttgcggcga taaaatgccg gagcattcta
    21961 tggtgcaatt tcatgataaa tggcactcct tcggttagtg aaacgagaaa accagataaa
    22021 gttcgattgc ggcctggcac aggggcgcta attatactca tctctgcgcc gctggggagg
    22081 ggaaatttag gggtaatagt aataaattgt atgcaaaagc cacgcaaaaa gcatggctct
    22141 gaatattttt gcacttaaat atgatgaata atcactttgt tgcccccctc gcttttcgct
    22201 tcgcgtaagg ctttatctgc ttccgtcatg acaagcgaga tattacgcgt gtcacccgtt
    22261 gaaaaaaccg tgccaatact gatggtcatt tgttcaggaa catcatatct gttactaaag
    22321 ccggtcagga gttcgacatt tttacgcata cgttccgcaa tgatttttgc ctgggcacta
    22381 ttgagttcgg taaatagcaa gccaaacacc tcaccctcca gtcgcgctaa aatatcatcg
    22441 gggcggatgc tttgctggat aatatcgaca attgaaacca gcactttatc acccacacga
    22501 tgtccccatt gggcattgat gcgtttgaac tgattaatat caaccagcat gacacagaag
    22561 tccttaacaa caggtcgcga caataatgac tctacgctgt tgaaaaagta gcgccgatta
    22621 taaatattag tcagcacatc atgaactgcc agtttgctgg agagttgcag ttcctgaaaa
    22681 atgttataaa tcagaaaaga cacaacaaac agtttactgg aaacttcaat cccgcgactg
    22741 atataccaga tggtgtaatt atactcatcc agagtcagta gaagtaagtt gcagcatagc
    22801 gatgccagac ataataatgc tattaacggc catagttcgc tggctaatcg tgtacgcatg
    22861 ataataaaga acagtaaaaa tgcccacaga caaaccaata tttttgtata gttgattccc
    22921 caggtcgcag tatggttgtc aggacagtag tcgacgacat acaaagaata gtcagcgtta
    22981 taactgctca gattgtgtgc cagaagggga aaaactaaaa aaggaatcag cgccagcaac
    23041 aggattcccg tttttttctt attgttgtca aggatgttgt cttttccata acaaaagagt
    23101 gccagcgagg ttaaacaaat aaaactgagt tggcggaaaa tatagaaaat agaaacatca
    23161 ttcgactttg tctggatcag tgtactgccg ttaattggtt tatggataat tacaatggtt
    23221 tcaactaaat ag
//
LOCUS       DAJOWB010000070        23162 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJOWB010000070 DAJOWB010000000
VERSION     DAJOWB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.070"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..3028)
                     /locus_tag="OHO85_002942"
     CDS             complement(<1..3028)
                     /locus_tag="OHO85_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCQ2672289.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQHCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGEGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSDTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYVRSVNTVGKS
                     TFVEAVGRASDDAEGYLDFFKGLITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWAVKIEQTKDGKHYVAGLGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTN"
     gene            complement(3089..3697)
                     /locus_tag="OHO85_002943"
     CDS             complement(3089..3697)
                     /locus_tag="OHO85_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCQ2672290.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGLFQAVLGAALIAVA
                     WWNPAGWLGAAAITGMYGAGASMILGGVAQMLAPKPRTPRTQTTDNGKQNTYFSSLDN
                     MVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(3634..4377)
                     /locus_tag="OHO85_002944"
     CDS             complement(3634..4377)
                     /locus_tag="OHO85_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2672291.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTSEGERYFPCVNISGEPE
                     DYFRMSPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTARRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNMEAT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRESAFTGIYNDLAAASTCV"
     gene            complement(4383..5081)
                     /locus_tag="OHO85_002945"
     CDS             complement(4383..5081)
                     /locus_tag="OHO85_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCQ2672292.1"
                     /translation="MQDIQQETLNECIRAEQSASVVLWEIDLTEVGGDRYFFCNEQNE
                     KGESVTWQSRQYQAYPIQGSGFEMNGKGASARPTLKVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTADITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(5081..5419)
                     /locus_tag="OHO85_002946"
     CDS             complement(5081..5419)
                     /locus_tag="OHO85_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2672293.1"
                     /translation="MANTEEFHWLPEDGMKTENKPSIKTVRFGDGYEQRSPNGLNHSL
                     RVFTCDFKVEANERDSFEKFLARHEGYKSFFWRPPGINRKIRVVCRTWSATEHITYTD
                     FSCQFDEVVI"
     gene            complement(5412..8645)
                     /locus_tag="OHO85_002947"
     CDS             complement(5412..8645)
                     /locus_tag="OHO85_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCQ2672294.1"
                     /translation="MAEQTSRLAIIIDSTGAKNNADNLTSSLVKMTQAGETAANSAGK
                     VTKATEDEKNALAKLKAAIDPVGAAIDTVGRRYSELKKFFDKGLIDKEEYEFLVRKLN
                     ETTEELSGVAQAQREAEKAGKLAAAQQEAQAQAFQRMLDKIDPLAAALRNLEQQHDEL
                     NAAFASGKINGSQFENYSRKIQETRRELTGEAQAEREAAKAHDEQVVALQRLIAQLDP
                     VGTAFNRLVEQQKQLNEAKAKGMLSPEMYEELSGKLRAMRSELEVTQSQLSKTGMSAK
                     QTAFAMRMLPAQMTDIVVGLSTGQSPFMVLMQQGGQLKDMFGGIGPAIKGVGSYVLGL
                     INPFTLTAAAVGVLGLAYYKGSQEQDEFNKSLILTGNQLGTTSGQLGDIAQRAGNAAD
                     STTGAAAAVLNQLVRSGKVASSSLEQVTTAIVKTSEVTGISTEQLVNDFNEIAKDPVS
                     AISKINDQYHFLTLATYNQIKALQDEGNQQEAARIATEEYSSSMIQRTNQIKENLGYL
                     ETAWKAVADSAKWAWDSMLDIGREASLDQKISDVLRQIDEIEKNTRPGVFGLGGIGDG
                     GAQNKRLARLKQQLGVLQAEKIAQDVLNSSINDYNKRQQEGIELRQRADAFSKQYQTR
                     EQQRASELAKLEKLKNQYSKEEYNNLIAQINERYKDPKQPKAKGYSDDAAQRVIDHLN
                     QQNALLSSQAELTVKLSSSEQELVKWRQQIADLESRPSSKLTQDQKSLLLHREEITAL
                     MEKNVAIEKNNRLIKESAEIAAWRDSLQASIDNRQQGYDIQIAGYGVGDKNQQRQQEL
                     LRIEREYNNQRLQLERDYADKSRGMSNHVFHEKMQALNDALEREKEIVRKKNEQLDIQ
                     AGDWVSGASQGFNNWLDDTKDISEQIKSTTTQMFDGMTDALGDFVTTGKANFRSFATS
                     VISDLSRIALKASITGIFDSISNSSSGGILGTIGSAISKFIPNVKGGVYESPSLNTYS
                     NGIYDSPQFFAFAKGAGVFGEAGPEAIMPLTRTSDGSLGVRAINSKSGNGGGDITYAP
                     VYQITIQNDGQNGEIGPQAIKALMGMVDQRVQGNLLNMRRDGGMLSG"
     gene            complement(8811..9011)
                     /locus_tag="OHO85_002948"
     CDS             complement(8811..9011)
                     /locus_tag="OHO85_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672295.1"
                     /translation="MLKELLYAYSVISRARRYAGMAGVPLPLSLTEINEYLATHPVLI
                     ERDEFEAVIFALDDQYFQEQCV"
     gene            complement(9119..9478)
                     /locus_tag="OHO85_002949"
     CDS             complement(9119..9478)
                     /locus_tag="OHO85_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672296.1"
                     /translation="MVASSDNDQYRSRNALIRRHIEKMDASLHVGTKEFDISKVSEVD
                     SVDDLLIDNAARYLLKDWKGVGELVNGVEVALEYTAERGIALLKQNPELYWQILAEAA
                     SIAQGKEQQKQDTIKKP"
     gene            complement(9661..10590)
                     /locus_tag="OHO85_002950"
     CDS             complement(9661..10590)
                     /locus_tag="OHO85_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672297.1"
                     /translation="MSSGAKVISAFIRETVAGTTPASGDWSLLKRTSWGVKPTQNKGE
                     NNEIGGSRMAQGATPGTVDVGGDVGTKFRWGQHDDFLASCFGAEWSGDSLTMGNERIT
                     FSLATYASDVGIASVVRGAQVGSWKMQIPNDGDITATVTFAGLDWESKADDTNFIKGE
                     PVDSAGKLRYSFKEVSAVSLNGVAGGNGFCIDSFDIQFDNKLQTQRCIGTGSPYAGAN
                     IPTTFTPSGTVTLSWSKAAWEIWSKTLTGETVPFSFTLSNGEGAYTFSFPKVQVSGEW
                     PDGGNSDIIQVQLSITAADEAPTITRKKIPRLP"
     gene            complement(10617..11009)
                     /locus_tag="OHO85_002951"
     CDS             complement(10617..11009)
                     /locus_tag="OHO85_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HCQ2672298.1"
                     /translation="MTFTEIRNTVISRMTAQTIIDGKDVLYPNAPTFDPSGKLIWARL
                     SNIPGQAGVNEIGAGPIVYRTGIIIIQLFVPAGSGSKLITETADKLRELFEFQDDDRL
                     SYQAVSSIAVGEKNGWFQLNLQIPYRAL"
     gene            complement(11006..11386)
                     /locus_tag="OHO85_002952"
     CDS             complement(11006..11386)
                     /locus_tag="OHO85_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCQ2672299.1"
                     /translation="MAKAWDIEPSIFAGMIEEDVGLKIRYIAMQILTAIDIAAPVDTG
                     RFRNNNLVSLQHPDFGISDNVDPNGTIAVQRGIGVISKAANYGVIYIQNNLPYAEALE
                     NGHSQQAPTGVYANAFHGVLQAYK"
     gene            complement(11387..11770)
                     /locus_tag="OHO85_002953"
     CDS             complement(11387..11770)
                     /locus_tag="OHO85_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCQ2672300.1"
                     /translation="MGLREEIQSEVAAAFDEDLADAVSDFSGSYVTHRHWDPVTETGG
                     ESTAIYTGRGVLTRYKLGRIDGINILHGDLKLTALVCEVTDKPAVDHIIEIYDPVLRQ
                     LQRYEVITASVDPSASVYSIQLRRA"
     gene            complement(11770..12165)
                     /locus_tag="OHO85_002954"
     CDS             complement(11770..12165)
                     /locus_tag="OHO85_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein singed"
                     /protein_id="HCQ2672301.1"
                     /translation="MITYVTCDDVDNAFGNAWTSENAKNKAVLMANAWLNGFNLKINP
                     SRIPEEVKLAGAYAARIASFGKLFQQKNDSGVVISKAVSADGVSVSKSFAELPANSTV
                     LLEPDLQLAIALLKPYGLSRSQVRVVRGG"
     gene            complement(12169..12345)
                     /locus_tag="OHO85_002955"
     CDS             complement(12169..12345)
                     /locus_tag="OHO85_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004121546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672302.1"
                     /translation="MAGLTKEQRAQREAAQKTQIELVVMVTDYQMFPGAPTIANVHPD
                     EVDNWKAMGWKTQE"
     gene            complement(12388..13527)
                     /locus_tag="OHO85_002956"
     CDS             complement(12388..13527)
                     /locus_tag="OHO85_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 coat - protein 5 family protein"
                     /protein_id="HCQ2672303.1"
                     /translation="MAGNTLTGLIPTIYTALDIVSREQVGFIPAVAKNAKADAAAKDQ
                     TVTAPVAPEAKTEDIVPGPSAPNTGDQNIGTVDVKITKSKMAPVKWNGEEQLALGPSG
                     TYNTILADQFKQAFRALANEVEADLGALYFGASRAVGTAGTTPFGVKDDLSDAALARQ
                     VLEDNGTPTTDLQMVLGSTAIANLRGKQSVLFKVNESGTEQLLREGVLGRLEGFNIHS
                     SAGVKRAPKVAATGYLVNGEKKEGDVLISIDTGSGSISAGQIVTFAGDPNQYVVAAAT
                     SNLITLAAPGLRQDLADDTAITVLGSFTANMAFDRNAFLLASRTPAMPEGGDNADDVM
                     NVTDPISGITFQIALYRQYRQVRYEVGLAWGVSSVKPAHGCLILG"
     gene            complement(13626..14390)
                     /locus_tag="OHO85_002957"
     CDS             complement(13626..14390)
                     /locus_tag="OHO85_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672304.1"
                     /translation="MKLKTVEINGKQYAEIDTAGLPVYVHDDGKEIGFDAPLAIKKIT
                     ELNGEAKNHRLAKEAAEEKLAKFAAIEDPKKAIEALEMLSKIDQKKLIDAGQVDQVKA
                     EITKNFQQQLDEEKQRSQMLETQLYDSMIGGSFAGSKYIADKIAIPADLLQARFGQAF
                     KVEEGKIVAYDASGNKIYSRAKPGELAQFDEALEFLVENYPQKDYILKASGNNGGGSR
                     PTQHDIGQKTMKRSAFDALDVAGKQNALKDGITIVD"
     gene            complement(14495..15607)
                     /locus_tag="OHO85_002958"
     CDS             complement(14495..15607)
                     /locus_tag="OHO85_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001645719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672305.1"
                     /translation="MQTVNERLRDESIAHAVWISRYSTGVAARMVKILNDSDAELTAR
                     LLVALDSLDPGSFTVTRLESLLASVREVNRAAINSMFTSLSGELNELAIYEAGYQLSL
                     FDSLLPDFVADVHPLVGISSDALYAAAMARPFQGRLLSEWASDLEADRLRRITNTVRQ
                     GFLLGDTNEQIARKIRGHVSKGFQDGALQMSRANAASIAKTAVGHLAATARESFASAN
                     NDLIKGKQWLSTLDNRTTPQCRIRDRLKYTLDNKPVGHSVPYLQGPGKIHFCCRSTET
                     FILKSAKELGIDVRDISPAERASMDGVVAGDTTYREWFLRQPYTRQKQIVGETRAKLI
                     RDGGMSPDEFYTDKGEWLTLKQLRERDAQVFRKAGI"
     gene            complement(15591..16997)
                     /locus_tag="OHO85_002959"
     CDS             complement(15591..16997)
                     /locus_tag="OHO85_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4055 domain-containing protein"
                     /protein_id="HCQ2672306.1"
                     /translation="MADISTPNLDYNDMLEAWDINDALMGGTLEMRRQGENYLPKWPN
                     EDEDAYKKRLSVATLLPVYEESIKQNIGRIFAEPTVLSEETPEKIREYAENIDMEGSR
                     LDVWAQQFFSLAFQYGVAHALVDYPRTDMNEIRTKADENAAGGRPYVTMLNPRQVIGW
                     KSKVEKGKVVLTDLRIKEVIIIDGDDFGQKKVEQIRHIMPRRVEIYRRSEGTNGESVW
                     MLHESWNTSRDDIPLVTLYTKKTGFMRGTPPLLNLGLLNIKHWQSQSEQDNILHVARV
                     PLLVAYGLDRNEELTVGASTATIFEDRTKNGLEYVEHSGAAIESGETSLEKLENQMRH
                     AGAKLLRAENTSTKSVDQTNEERMQEHSPLYTMANSLEDALDNILQIMAEWSGESCGG
                     NVDVRTELDVSAQVFDSSSALAVQSLRQGGDIRQIDAVRVLQALKFIDQDSRPEEVID
                     ELKNQSVMLMEINDANRE"
     gene            complement(17000..18301)
                     /locus_tag="OHO85_002960"
     CDS             complement(17000..18301)
                     /locus_tag="OHO85_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HCQ2672307.1"
                     /translation="MISPTLNVPQARFLSMPHKFKAYIAGFGSGKTWVGCGGICKGIW
                     EHPSINQGYFAPTYPQIRDIFYPTVEEVAADWGLNVKINEGNKEVHFYYGRQYRGTTI
                     CRSMEKPQTIVGFKIGNALVDELDILPKEKARTAWRKIIARMRYKIDGLRNGIDVTTT
                     PEGFKFVYEQFVKAVREKTELASLYGLVQASTFDNEKNLPADYIPSLLESYPPELIKA
                     YLLGRFTNLTSGTVYHQFDRKLNNCEEVEQPGEPIYIGMDFNVGKMAGIVHVLRLGLP
                     CAVTEIINAYDTPDMIRLIKERFWLYDGHDYRKVREIYIYPDASGDSRKSSNASTTDI
                     AQLKQAGFNVVVNSSNPPVKDRVNSMNAMFCNANGERRYKVNVKRCPVYAESLEQQVW
                     DDKGEPDKKSGNDHPNDAGGYFIVKQFPIVKPTGRVTSLRI"
     gene            complement(18282..19376)
                     /locus_tag="OHO85_002961"
     CDS             complement(18282..19376)
                     /locus_tag="OHO85_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCQ2672308.1"
                     /translation="MSRPDWGALQQEYIAEYTRSGISPVAWCEARGLNYATARRYIKK
                     TPKNAQKEMRKTAQKSARKKTAQTAQRQNGKSQKKKAVSDALLIEGDTEEISFCPDEF
                     GISDQQAKFAMLVAQGKKPTEAYRLAGYEGQGATANSNASRMLRNARVYRAISYFRNQ
                     YQKRYTADLDLLVSQLMAIVQADPNQLAQFRRVNCRYCWGENHLYQWRDIAEFDKAAA
                     QASRDGKPEPEYGGLGFVDNAIPNPDCPKCCGEGTGQLYMADTTLLDGDARQLYAGSK
                     LGKYGVEILLEDKAAARRELLRLLSAGGALCADKRLQELEIERRRIENQKLRKEIETV
                     EDNEHPQPVAININVVDARLRSDEDDLSDA"
     gene            complement(19380..19631)
                     /locus_tag="OHO85_002962"
     CDS             complement(19380..19631)
                     /locus_tag="OHO85_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672309.1"
                     /translation="MIPYSKVESLAACRMTAQQIADVLDVDLNRLKENREAMTDFYAA
                     IRKGRAKGEAELRAALFKLARKGDAFALRELLRVDKNQD"
     gene            complement(19567..20499)
                     /locus_tag="OHO85_002963"
     CDS             complement(19567..20499)
                     /locus_tag="OHO85_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672310.1"
                     /translation="MRDDFCAFILTHGRPDKVLTYRTLRRAGYTGKIFIVVDDEDKTR
                     HQYMAEFGEQVLVFSKADIASRFDEADNFGDRRSIFYARNACFDLAKLVGCKYFIQLD
                     DDYHEFQFRVDRNYDQAYFPIRKLDAILSEMLAYYESIPALSIAMSQGGDFLGDNGGH
                     ASWVKRKAMNSFICSVDRPFSFMGRINEDVNTYTNLGRCGELFMTIGAVQLGQKQTQK
                     NSGGMTELYLDSGTYVKSFYSVMYSPSCVKISLMGASHKRIHHQVTWNNAAVKILHEK
                     YRKKTPCISMGVTNDSVFESRVSGSVPDDCTTNR"
     gene            complement(20492..21286)
                     /locus_tag="OHO85_002964"
     CDS             complement(20492..21286)
                     /locus_tag="OHO85_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCQ2672311.1"
                     /translation="MSEKLKIVYRPLQELSPYAHNARTHSTEQVAQLVESIKQFGWTN
                     PVLIDEKGEIIAGHGRVMAAEMLKMDSVPVIVLSGLTDEQKKAYRLADNRLPMNAGWD
                     EDLLRMELSDLINADFDVSLTGFGPTEIDELLTDVLSGTGNKEEPYTTKIDTPVYEPS
                     GGKPDIRELYDDTKTQELISRIRSASLDPDIEKFLLCAAERHTVFNFSRIADYYAHAP
                     AEIQCLFEESALVIIDYQQAIENGFVRMTQRMVEIMHGGEEEEEYA"
     gene            complement(21424..22881)
                     /gene="trkG"
                     /locus_tag="OHO85_002965"
     CDS             complement(21424..22881)
                     /gene="trkG"
                     /locus_tag="OHO85_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkG"
                     /protein_id="HCQ2672312.1"
                     /translation="MNTSHVRVVTHMCGFLVWLYSLSMLPPMVVALFYKEKSLFVFFI
                     TFVIFFCIGGGAWYTTKKSGIQLRTRDGFIIIVMFWILFSVISAFPLWIDSELNLTFI
                     DALFEGVSGITTTGATVIDDVSSLPRAYLYYRSQLNFIGGLGVIVLAVAVLPLLGIGG
                     AKLYQSEMPGPFKDDKLTPRLADTSRTLWITYSLLGIACIVCYRLAGMPLFDAICHGI
                     STVSLGGFSTHSESIGYFNNYLVELVAGSFSLLSAFNFTLWYIVISRKTIKPLIRDIE
                     LRFFLLIALVVIIVTSFQVWHIGMYDLHGSFIHSFFLASSMLTDNGLATQDYANWPTH
                     TIVFLLLSSFFGGCIGSTCGGIKSLRFLILFKQSKHEINQLSHPRALLSVNVGGKIVT
                     DRVMRSVWSFFFLYTLFTVFFILVLNGMGYDFLTSFATVAACINNMGLGFGATASSFG
                     VLNDIAKYLMCIAMILGRLEIYPVIILFSGFFWRS"
     gene            complement(23078..>23162)
                     /locus_tag="OHO85_002966"
     CDS             complement(23078..>23162)
                     /locus_tag="OHO85_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HCQ2672313.1"
                     /translation="PPAWIMQHPPDWQTPLNGIISPSGNDW"
BASE COUNT         5753 a         6368 c         4906 g         6135 t
ORIGIN      
        1 tgttcgtgcg ggggaaagac ttgctgaccg ttttgacaat atcgccttca atctgattgg
       61 ctgacagttt ccccttaatc tgacagttct cattaatcgt gacgttgttg agcgtcccgg
      121 agttcgcatt cacactgcca ctgatatccg catttttagc agtcagcttt ccgtccggtg
      181 tcagggaaaa tgccggtgga tttccaccgc tggtaatggt gggggccgtc aggcgcttca
      241 ggaacacgtc gttcatgaat atctggttgc cctgcgccac aaacatcggc gtttcattcc
      301 cgtttgccgg gtcaataaac gcgatacggt tagcggcaac cagaaactgg ctcagtttgc
      361 cttcctctgt atcctccatg ctgaggccaa gtcccgcgac ataatgtttg ccgtctttgg
      421 tctgctcaat tttgacagcc cacatggcat tccatttatc gttggcatcc ttccactctt
      481 tcgaaaactc atccagtctg ctggcgttat cctccgtcag gtcgactttt tccagcagct
      541 ctttaccaag atgggattca gttatcaggc ctttgaaaaa atccaggtaa ccttcggcat
      601 catcgctcgc ccgaccgacg gcctccacga atgtcgattt gccgactgtg ttcacactgc
      661 gaacgtaaaa ataataatca tggcccggct taatattgat actggcggct atccagtaca
      721 gcgccgtgcc aagatagcgg gctgtggttt caacctgcct gatatcagca atccgctttt
      781 ccgagaacca gaactcaaat tgtaccgtcg gatcataaac cgcaagatgc ggcgtggcgg
      841 ttatctgaaa atagcccggt gtcagctcaa tccgagacgg cgctgccggt gcggcaatcc
      901 ggaacgatac cgatgccgga tcgccctgct gcccccacgc atttactgcc cggacagtca
      961 gcctgtagtt ccccagcgcc agttgcgtga agcggtatgt ggtttccgtc gtcctggccg
     1021 tactgaccag ccgctcactg ccgtcatccg ctgccacggt caggcgaagc ataaagctca
     1081 cccccttcac caccttcggc gtgtcccatc gcgccagtac ctgatattcc ccgctgtctg
     1141 cggtgacttc ggcagtcagg tgctgcaccg ctggcggcgt gacaccattc accgtgtcac
     1201 tctggtcgcc gtcaaagtgc gccccgttat ccacgatggc ctctttttcc ggcacatgct
     1261 gcacggcggt gatggcatac gtgccgtcgt cgttctcacg gatactcaca cagcggaaca
     1321 ggcgctggcg caacgtcggc agcttcagcc cccacacgct gtattcagca acgccgtcag
     1381 gaacacggct cactttcacc ttcacgccgt cggtgacgga ctggacctcc acgctgaccg
     1441 gattaccctc accgtcaacc aggcttatca gcgtggtgcc ggaggatggc agcgtgattt
     1501 cacggtcgag cgtcagcgtc cgggtctggc tgttcaccgc cagcacgcga ccaccgatgc
     1561 tgataccggc atagtcatca tcgcagattt caataacatc gcccggcaca tggcgaagcc
     1621 cttctgcgcc cacgctgaaa tccacggtct gcgtttccag cagttccgtt ttaatcagcc
     1681 acagcccggc tcggtgtgcc tgcccccgac tggtacaacc aaaggcatcc atcttcgtga
     1741 cattacgacc gtaacgggca atggcctgcg tatcttcaac aagctctgtc gccgtctccc
     1801 agccgttgtt cgggtcaatc cagttcacct caacggcatt atggcggtcc ttcagggcgc
     1861 tgaagctgta gcggaacggc gcgccatcat ccggcatcac cacattactg cggttatagg
     1921 tccacacctt atccgactgt cggtcctgca cgaacgtcag cgtctgcccg ttccataccg
     1981 gcatacagcg catcgccgag cagaaatcac tgagcacatc ccacgccttg cgctgtgtgg
     2041 tcaggtaagc gttacaggtg atgcgcggct ccgtgccacc aaagccgtcc ggcaccgact
     2101 gatcgcaatg ctggccgatg acatacagcg cccatttgtc cacatccgcc gcaccaagac
     2161 gtttccccat gccgtagcgc ggatgggtca gcatatccca cagacaccag gccatgttgt
     2221 tgctgtatgc tggcttaaac gttccgtccc agataccgct gtattgccgc gtctgcgggt
     2281 tatagttcga cggcacctgc agaatgcgcc cgcgcagatg ataattacgg ctcacctgct
     2341 ggctgccgaa ctgctccgaa tccacctgca cgccgaccag tgccgtgttc gggtagcact
     2401 gtttcacatc gatgatttcg gtgtatgacg accagagcgt tttgttctgc aactggtctg
     2461 tggtgctgtc cggcgtcatc ctgcgcatcc ggatattaaa cgggcgcggc ggcaggttat
     2521 ccaccaccac cgaggccaga tactgcgagg tggttttgcc cttaatggtg atgtcttttt
     2581 ccgtcaccca gccaccgtta cgctgtatct gaaccagcag gcggacttcc gacggattcc
     2641 ggtcaccctt tgaggtggtt tccaccagtg cctgcacacc gaaggtaaag cgcagacggt
     2701 cgatgtttgc cgacgtgatg gtccgggtga tcggcgtgtc atatttcact tccgtaccca
     2761 gcaccgtctc ggagccggag gattcaaatc cctccggcgg tgtctgctcc tgctcaccgg
     2821 cccggaacac caccgtgacg ccggagatat tggtattccc ctcactgtcc agcaccggcg
     2881 tactgttcag cagcacgctt tttaatccat ccaccggacc ttcaaccggc ccttcgctga
     2941 tggcatcgat cacactcagc agctgcgtgg acttcaggtt gtccttcgct tcgcgcgggg
     3001 tatgcccctt actgctgcct ttacccattc gtcacactcc ataaacgaca aaaccgcccg
     3061 caggcggttt cacataaaac attttgcatc agcgaccaat caccacaacc tgaccaccgt
     3121 ccccttcgtc tgccgtgctg atctcctgag aaaccacgcg tgaccccacg cgcatttccc
     3181 cgtacagaac aggcagaaca ttgccctggg caaccatgtt gtccagtgac gagaaatacg
     3241 tgttctgctt accgttatcc gttgtttgtg tgcggggagt tctgggttta ggggccagca
     3301 tctgggccac accgccaagg atcatactgg cccccgcacc atacattcct gtaattgcgg
     3361 cagcccccaa ccaacctgca gggttccacc atgccaccgc aatcagcgcc gcccccagca
     3421 cagcctgaaa aagaccgcca cttttggctc ccgccagacg cggcacaatg tggatcacgg
     3481 caccatttgc cagcggttca ttaagacggg ctgataattc agtttcacct gcatcacgcc
     3541 cggcaatgcg tacctgatac cagccgtcgc tcagtttctg acgaaacgcc ggaagctgtg
     3601 tggccagtgc ccggatggct tcggcccccg ttttcacacg caggtcgatg cggcggccaa
     3661 atcgttgtaa atccccgtaa aggcagattc gcgccatgcc cggtgacgcc agagggagtg
     3721 tgtgcgtcgc tgccatttgt cggtatacct ctctcgtttg ctcagttgtt caggaatatg
     3781 gtgcagcagc tcgccatcac cacagtaaat ggcggcatga ttcggcaccg atgaaccaaa
     3841 acagcacagc agcacatcgc ccggctgcgc ctctgtcagt gcgacacggt aaaaaccagt
     3901 cgcctccata ttgtcaagat aaagattctg gccgttacgc caccagtcat ctccgcgatg
     3961 aaaatccggc atctcaattc ccgccagatg gtatgcatcc cggaacagcg tgtaacagtc
     4021 cgtcaccccg tgctcaaagc gccgcgcggt aagatgtggc acacagcgga acttgtgaat
     4081 cgccccgcgg cagaccagcc accacggcaa atcactctgc acctgcagcc gccgatcggc
     4141 ctcactcagc cagggcagac caccgggatg actgtggacc agcgccacaa tctcaccctg
     4201 catctctgcc cgcagccagt cctccggcga catccggaaa taatcctccg gctcaccgga
     4261 gatattcacg cagggaaaat atctttcccc ttccgacgtt ctcaccacga agccgcacga
     4321 ctccgctggc gcacatcgcc gggcgtgcgc cagaatcgct gattctgtct ctgtcatggg
     4381 atttactgcg aaagtttgtt aatggaaagg aagccgccaa agttgccgac attattgcgg
     4441 aacttacagc cactcaggca tttgctgcat ttatccttcg tgatatcggc tgtcggctgg
     4501 tcatattcat ccgcgaccgc cggaccgtga taaccgcact catcaccgcg ataggtccag
     4561 gtgcaggtat tagcaagcat gatgcgcccc ggaaaaacag cgccgtccgt ttccgtcggc
     4621 gtggacagta caaaggaggc actgaccgcg ctcagttcgc tgcactgctc aatacgccag
     4681 cggctgatca cctcctgctc cggatcggcg tcactgtttc cgttgacgaa gttcaccgca
     4741 tccagaaaac gggcgtaaac cttacgcctg accaccgttc cgccgaccag actctgcagg
     4801 tcttccgcca tcccggtgac catgccgtgc agattagaga ctttcagcgt tggccttgca
     4861 ctggcacctt tgccgttcat ctcaaatcca ctcccctgaa tgggataggc ctgatactgc
     4921 cgactctgcc aggtgacaga ctctcctttc tcgttctgct cattgcagaa aaagtagcga
     4981 tccccgccta cctcggtcag gtcgatttcc cagagcacca cgctggccga ctgctccgca
     5041 cgaatgcatt cattcagcgt ttcctgttgt atgtcctgca tcagatcacc acttcgtcaa
     5101 actgacacga aaaatcggta taggtgatat gttctgtcgc tgaccacgtt cgacacacca
     5161 ctctgatttt tctgttaata ccaggcgggc gccaaaaaaa agatttatag ccttcatgcc
     5221 gggctaaaaa tttttcaaat gaatcacgtt cattcgcctc aactttgaaa tcacaggtga
     5281 aaacgcgcag agaatgatta agtccatttg gacttcgttg ctcataacca tcgccaaatc
     5341 ttacagtttt tatcgatggt ttattttctg ttttcattcc atcctctggt aaccagtgaa
     5401 attcttccgt attagccact taacattccc ccatcacgtc gcatatttaa cagattgccc
     5461 tgcacccgct gatcaaccat ccccataagt gcttttattg cctgagggcc aatctctcca
     5521 ttctggccgt cattttgaat agtgatttgg tatacagggg cataggtaat atctccgcct
     5581 ccattaccac ttttactatt aatagctctg acaccaagag aaccatcgga agttcgtgtt
     5641 aatggcataa tggcttccgg accagcctca ccaaaaacac cagccccttt tgcaaaagca
     5701 aaaaattgcg gggaatcata aataccgttc gaatacgtgt tcaatgacgg agactcataa
     5761 acaccgccct ttacattcgg aataaattta ctaatagcac tcccgatagt tcctaaaatc
     5821 ccccccgaag aactgttact aatgctgtcg aaaatcccag taattgaagc ctttaatgct
     5881 attcgactaa gatccgaaat cacggaagta gcgaaagaac gaaaatttgc cttgcctgtc
     5941 gtgacaaaat cacccagcgc atcggtcatt ccatcaaaca tctgagtcgt ggttgatttt
     6001 atctgctcac tgatatcctt agtgtcatca agccagttat tgaatccctg ggaagcacca
     6061 ctaacccagt ctcctgcctg aatatcgagc tgctcgtttt tctttctgac aatttctttt
     6121 tcccgttcca gagcatcatt cagagcctgc attttctcat gaaaaacatg atttgacatc
     6181 ccacgggatt tatctgcata gtcacgttca agttgcagac gctgattgtt atattcacgt
     6241 tcaatccgca gtaattcctg ctggcgttgc tgatttttat cgccaacccc ataaccagca
     6301 atctgaatat catacccctg ctgacgatta tcaattgaag cctgcaatga atcacgccat
     6361 gcagctattt cggcggattc cttgattagc ctgttatttt tttcaatcgc aacatttttc
     6421 tccatcaacg cggttatttc ttcccggtgt aagagaagcg atttctgatc ctgggttaat
     6481 ttcgatgacg gtcgtgactc caggtcggca atctgctgac gccacttaac cagttcctgt
     6541 tcagaggaac ttaatttaac ggtcaattca gcttgtgaac ttagtaacgc attctgttga
     6601 ttcagatgat caatcactcg ttgggcagca tcatccgaat aacctttcgc ctttggttgc
     6661 tttggatctt tataacgctc atttatttga gcgataagat tattatattc ttcctttgaa
     6721 tactgattct ttagcttttc cagttttgca agttcactag ctctctgctg ctcccgggtc
     6781 tgatattgtt ttgaaaaagc atctgctctc tgtctgagtt caattccttc ctgttgtcgc
     6841 ttgttgtaat cgtttattga tgaatttagt acgtcctgag caattttttc tgcttgaagt
     6901 acgcccaatt gctgctttaa tcgtgccagc cttttatttt gagctccgcc atctccaatg
     6961 ccacctaacc cgaaaactcc gggtcgggta tttttttcta tttcatcaat ttgacggaga
     7021 acatctgaga ttttttgatc aagggaggcc tcacggccaa tatccagcat ggaatcccat
     7081 gcccattttg cggagtctgc gacagctttc catgcagtct caagataacc aagattttct
     7141 ttaatctggt tggtgcgctg gatcattgag gatgagtatt cttctgtcgc aatgcgggcg
     7201 gcctcctgct ggttcccttc atcctgtagc gccttaatct ggttataagt cgcaagtgtc
     7261 agaaaatggt actgatcatt aatttttgat atggcactga caggatcctt tgcaatttca
     7321 ttgaagtcat taaccagttg ttcggttgat attcctgtta cttcgctcgt ttttactatc
     7381 gctgtcgtca cttgctccag cgaactgctc gctacctttc ccgaacgcac aagctgattt
     7441 aatactgccg ccgcagcacc agttgtcgaa tcagccgcat tcccggcacg ttgagctata
     7501 tcgcccaatt gcccgctggt tgtccccaac tgatttccgg taagaataag agatttatta
     7561 aattcgtcct gctcctgaga gcctttatag taagccagtc ctaagacccc aacggctgct
     7621 gcggtcaggg taaaaggatt aattaatccc agcacataag aacctacacc tttgatcgcc
     7681 gggccaatcc caccgaacat atcttttagc tggccgccct gctgcattaa caccataaat
     7741 ggcgactgac cagtggacaa cccaacaaca atatccgtca tttgtgcagg caacatgcgc
     7801 atagcaaaag ccgtttgttt tgccgacatt ccggttttgc ttaattgtga ttgagtaacc
     7861 tcaagctcac tccgcatagc acgaagtttt ccagaaagct cctcatacat ttcaggagaa
     7921 agcatcccct tagcttttgc ttcattgagc tgtttctgtt gttctaccag acgattaaaa
     7981 gcagttccga caggatcaag ttgagcaatc agacgttgca aagcaacaac ctgttcatca
     8041 tgcgcttttg ctgcttctcg ctctgcctga gcctctccgg taagctctcg ccgtgtttcc
     8101 tgtatttttc ggctataatt ctcaaactga gaaccattta ttttcccgga tgcaaacgca
     8161 gcattaagtt catcatgctg ttgttcaaga tttcttagcg ccgcagccag agggtcgatc
     8221 ttgtccagca ttctttgaaa ggcctgagcc tgcgcttcct gctgggcggc agcaagtttt
     8281 ccggccttct cggcttctct ctgcgcttgc gcaaccccgc tcaattcctc tgtggtttca
     8341 ttaagtttac ggacaagaaa ttcatattct tctttatcaa taagcccttt atcgaaaaat
     8401 ttctttaatt cagaatagcg tcgaccgaca gtatcaattg cggcaccaac tggatcaata
     8461 gctgctttta attttgcgag cgcgttcttc tcatcttctg ttgccttagt cactttccct
     8521 gcgctatttg cagcagtttc cccagcctgc gtcattttga ctaatgagga ggtcagattg
     8581 tcagcattat ttttcgctcc agtgctatca ataattattg cgagacgcga ggtttgctct
     8641 gccatttatt aaaactcctg acaacaaaaa acccaccgcg aagtgggttt caggcgacat
     8701 aatagtagat atagcgatta cgaggccacg caatgctttt atccaggagc atcatcgatt
     8761 taattaaaga caccatcaca tctctgtaac agagtgtacg taattaacaa ctacacacac
     8821 tgctcctgaa aatactggtc atccagtgca aagatcactg cttcaaattc atcgcgctca
     8881 atcaataccg gatgagtggc taaatattca tttatctctg tcagagataa aggcaaaggc
     8941 accccagcca ttccagcata acgtcgggca cgggatatta ccgaataggc gtacaacaac
     9001 tccttaagca ccgggtctat ttctggttcc ggtatcggtg gcaacctgag tttttctcgc
     9061 ttccatcttg ccttttctcc cctttctccc ccgaactccg ataaccaccg ctgggcagct
     9121 atggcttttt tatcgtatcc tgcttctgct gctctttacc ctgggcgatg ctggctgctt
     9181 ctgcaaggat ctgccagtac aactctggat tctgcttaag cagcgcgatc cctcgttctg
     9241 ccgtatattc cagtgcaacc tcaacaccat taaccagttc accaacccct ttccagtctt
     9301 tcagcagata acgagcggca ttatcaatga gtaaatcatc aacagaatcc acctcggaaa
     9361 cctttgaaat atcaaactcc ttcgttccga cgtgcaaact ggcatccatt ttctcaatgt
     9421 ggcgacggat taatgcatta cgggagcgat actgatcgtt atcgctgctt gccaccaaca
     9481 gttttaaccc gtctacaggt tttaagtcct tcattggcgt aaaccagcgt tctccaccaa
     9541 tgataatttt ctgattaaga ataaacatcc ataacctcat tcaatgcgtc cccctgtagg
     9601 gcagtaccac aggaggaata acggaaaatc aactaatcgc ctcagcactg gcttttgcga
     9661 tcacggcagc cggggaattt tttttctggt tatcgtaggt gcttcatctg ctgcggtaat
     9721 gctcaattga acctggataa tgtcgctatt acccccatca ggccattcac ctgacacctg
     9781 aaccttcggg aaactgaaag tgtatgcccc ctctccattc gaaagcgtga agctgaacgg
     9841 aactgtttct ccagtcagtg ttttactcca gatttcccac gcggctttag accatgaaag
     9901 cgtcaccgta ccggacggtg taaaagtagt cggaatattt gctcctgcat aaggcgagcc
     9961 agtcccgata caacgctgtg tctggagttt gttatcgaac tgaatatcaa aactgtcgat
    10021 acaaaaaccg ttacctccgg caacaccatt caggcttact gctgaaacct ccttaaacga
    10081 ataacgtagc tttcctgcac tatccacagg ttcgcctttg ataaaattcg tatcatcggc
    10141 ctttgattcc cagtccagtc cggcaaaggt tacggtcgct gtaatatcgc cgtcgttagg
    10201 gatctgcatt ttccatgagc caacctgcgc tcctctgacg acagaggcaa ttccgacatc
    10261 ggacgcatag gtcgccagag aaaatgttat tcgctcattc cccattgtca gagaatcgcc
    10321 tgaccattcc gcgccgaaac aggatgcaag aaaatcatca tgttgacccc agcgaaattt
    10381 ggtaccaaca tcaccgccga catccacagt gccaggcgtc gccccctgag ccatccggga
    10441 gccaccaatc tcattatttt cgcctttatt ctgggtgggt tttactcccc agcttgtgcg
    10501 ttttaataaa ctccagtcac cacttgctgg cgtagtgcct gcaaccgtct cccggataaa
    10561 tgccgagata acctttgcac ctgaactcac aggagcctcc tatgtcatta attgcgctag
    10621 agcgcgcgat atggaatttg aagattaagc tggaaccagc cattcttttc gccaacggct
    10681 attgaggaaa cagcctggta actgagacga tcgtcatcct gaaactcaaa cagttcccgc
    10741 aatttatcgg ctgtctcagt aatgagtttt gaaccagaac ctgcggggac aaataactga
    10801 ataatgatta tccccgtgcg ataaacaatc ggccccgcgc caatttcatt aactccagcc
    10861 tgcccgggaa tattacttaa ccgcgcccag attaacttac cggaaggatc gaacgttggc
    10921 gcgttcggat acaatacgtc ttttccatca ataatcgtct gtgccgtcat tctggaaata
    10981 accgtatttc tgatttcagt aaacgtcatt tgtaagcctg taaaacacca tgaaaagcgt
    11041 tggcatacac gccagttggc gcttgttgtg aatgaccgtt ttcaagagcc tctgcataag
    11101 gaaggttatt ctggatataa ataactccgt aatttgcagc tttcgaaata accccgatcc
    11161 cacgctgaac cgcaatcgta ccgttcggat ccacgttatc agatatacca aaatcgggat
    11221 gctgtaacga caccaggttg ttatttctga aacgtccggt atcaaccgga gcagcaatat
    11281 caatagcagt aagaatctgc atagcgatgt agcgaatttt cagccccaca tcttcctcaa
    11341 tcatcccggc aaatattgac ggttctatat cccatgcctt tgccatttac gctctcctta
    11401 actggataga gtaaactgat gcggaaggat ctacactcgc tgtaattacc tcgtatcgtt
    11461 gtaactgcct taatacagga tcataaatct caataatatg gtcgacagcg ggtttatccg
    11521 taacctcgca taccagagcg gttaatttaa ggtcaccatg caaaatatta atcccatcaa
    11581 ttctgcccag cttataacgt gtcaacacgc ctcgcccggt atagattgcg gtggattcgc
    11641 cgccagtttc cgtcacagga tcccagtgcc ggtgcgtaac gtaagaacca gaaaaatcac
    11701 tcacggcgtc cgctaaatcc tcatcaaaag cagcggcaac ctctgactga atctcttcac
    11761 gaagccccat tatccccccc tcacaactct gacttgtgag cgactaagtc cgtacggttt
    11821 cagcagtgct atcgcaagct gtaaatcggg ttcaagcaat acagtgctgt ttgctggcaa
    11881 ctcagcaaat gatttcgata cactgacccc gtcagccgac acggctttac tgataacaac
    11941 gccagaatca tttttctgct gaaacaactt accgaatgag gcaattctgg ctgcatatgc
    12001 tcccgcaagt tttacctctt ccggaatacg ggatgggtta attttcaggt tgaagccatt
    12061 aagccaggca ttagccatca aaacagcttt atttttagcg ttctcactcg tccaggcgtt
    12121 cccaaacgca ttatcaacgt catcacaggt cacgtaagtg atcatgtgtt actcctgagt
    12181 tttccagccc atagccttcc agttgtcaac ttcatcagga tgaacattag cgatagttgg
    12241 tgcgccggga aacatctgat aatcggtcac cataaccact aactcaattt gcgttttttg
    12301 tgcggcttca cgctgtgctc tttgctcttt agttaatccg gccatatgcc ctccattaaa
    12361 aatggggccg aagccccgtt tgaatgttta accaagaatc agacaaccat gtgccggctt
    12421 cactgatgaa acaccccatg ccagtccaac ttcatagcgc acctggcgat actgacgata
    12481 cagtgcaatc tggaacgtaa tccctgaaat cgggtccgta acattcatta catcatcagc
    12541 attatcgcca ccttccggca ttgccggagt acgggatgcc agcagaaacg cgttgcgatc
    12601 aaacgccata tttgcagtaa aggagccaag aactgtgatt gcagtatcat cggccagatc
    12661 ctgacgcagc ccgggcgctg caagagtaat cagattgctg gtcgctgctg ccacaacata
    12721 ctgattcgga tcgccagcga acgtaacaat ctgacctgca gaaatactcc ccgaaccagt
    12781 atcaatggaa ataagaacat cgccttcttt tttctcgcca ttcacgagat aaccagttgc
    12841 agcaaccttt ggcgctcgtt ttacacctgc cgaactgtga atattgaatc cctccagacg
    12901 ccccaacacg ccctcacgca gtagctgttc agtgccggat tcattcactt taaacagtac
    12961 agactgtttt ccgcgcaaat tagcaatggc agtggagcca agcaccatct gcagatcggt
    13021 tgtcggtgtg ccgttatcct ccagaacctg acgagccaga gcagcatcag aaagatcatc
    13081 tttaacacca aacggcgttg tccctgcggt tcccacggcg cgggaagcac cgaaatacaa
    13141 cgcaccaaga tcagcctcaa cctcgtttgc aagggcgcga aaagcctgct tgaactgatc
    13201 agccagaatg gtgttgtaag tccctgaagg gccaagagcc agttgttctt caccattcca
    13261 tttaaccggt gccattttgg atttagtaat tttcacatca acagtgccaa tattttgatc
    13321 accggtattc ggagctgacg gccccggtac gatatcttcg gttttcgcct caggcgcaac
    13381 tggcgcggtt accgtctgat cttttgctgc ggcgtcagct tttgcgtttt ttgctaccgc
    13441 agggataaaa cctacctgct cacgggatac aatatccagg gcggtataaa tagtcgggat
    13501 caacccggtc aaggtatttc cagccataat taaatattcc ttaaaaattt gcgtaattgt
    13561 gaatagatgg agtaatgagc tatccagccc tgacaccagc tcccatccgg aagctgacaa
    13621 atgtgttaat caacaattgt gataccgtct ttcaatgcgt tttgcttacc tgcaacatcc
    13681 agtgcatcaa aagcagagcg tttcatcgtt ttctgaccaa tatcatgctg tgtcggacgg
    13741 gagccgccgc cattgttgcc actggctttc aggatgtagt ctttctgagg gtaattttcg
    13801 acgaggaact ccagcgcctc atcaaactgc gccagttcgc caggcttcgc gcgggaataa
    13861 attttgttgc cggaagcgtc ataagcaacg atcttccctt cttccacttt gaatgcctgc
    13921 ccgaagcggg cttgtaataa atctgccggg atcgcaattt tatcggcaat atattttgaa
    13981 cccgcaaaac taccgccaat catagaatcg taaagctgcg tctccagcat ctgagagcgt
    14041 tgcttttctt catctaattg ctgctgaaaa tttttcgtaa tttctgcctt aacctggtca
    14101 acctgtcccg catcgatcag ctttttctgg tcgatttttg acagcatttc cagtgcctcg
    14161 atcgccttct tcgggtcttc gatagcggca aacttagcca gtttttcctc tgcagcttct
    14221 ttagccaggc gatgattttt tgcctcgcca ttaagctctg taattttttt tatcgccagc
    14281 ggtgcatcga agccgatttc tttaccatcg tcgtgcacat aaactggcag gccagcagta
    14341 tcaatttctg cgtattgttt tccgttaatc tcgaccgttt tcagtttcat attagtacct
    14401 ggtttaagtc ttccgacagt tacgctgctc accatccgga tcgcagcaat aaaaaaggcc
    14461 acccaaaggt agccttttgt aataaatgat ttatttaaat ccctgctttt ctgaatacct
    14521 gtgcatcacg ctcacggagt tgcttcagcg tcagccattc gcctttatcg gtgtaaaatt
    14581 catctggcga cataccacca tcccgaatca gctttgcccg ggtttccccc acaatctgtt
    14641 tttgtctggt gtaaggctgg cgcaaaaacc attccctgta ggttgtatct ccggccacca
    14701 cgccatccat gctggcccgc tcagccgggg aaatatcacg aacatcaata cccagttcct
    14761 tcgctgattt cagaatgaac gtttccgttg agcggcagca gaaatgaatt tttcccggcc
    14821 cctgcaaata aggcacgctg tgacctacag gtttattatc cagcgtatat ttgaggcgat
    14881 ccctgattcg acattgtggc gtagtacgat tatcaagcgt tgataaccat tgcttaccct
    14941 taatcaaatc attattcgcg ctggcaaaac tctcacgggc agtagcagca agatgtccaa
    15001 ccgctgtttt tgcaatgctg gccgcattag cccggctcat ctgcaatgca ccatcctgaa
    15061 atcccttact gacatgtccc cgaatttttc ttgcgatctg ctcattggta tcccccagca
    15121 aaaaaccctg acgcaccgta tttgttatgc gtctgagccg atccgcctca agatccgagg
    15181 cccactcact gagcagtcgt ccctggaatg gtcgcgccat tgcagcggcg taaagtgcat
    15241 cagaagagat accaaccaga gggtgaacat cagcaacaaa atcaggtagc agagaatcaa
    15301 acagacttaa ctgataacca gcctcataaa ttgccagctc gttcagctct ccggagagac
    15361 tggtaaacat gctgttaata gcagcgcggt taacctctct gacactcgcc agaagtgact
    15421 ccaggcgcgt aacggtaaaa ctaccaggat cgaggctgtc cagtgctacc agcaggcgag
    15481 ctgtaagctc cgcatcgctg tcattcagta ttttcaccat tctggcagcc acaccagtgc
    15541 tatagcggga tatccagact gcatgagcaa ttgattcatc acgcagccgt tcattcacgg
    15601 tttgcatcat tgatttccat cagcattaca ctctgatttt ttaattcatc gatcacttcc
    15661 tctggacggg aatcctgatc gataaatttc aacgcctgca acacccgaac cgcatcaatc
    15721 tgacgtatat caccgccctg acgcaatgac tgaacagcca gcgcggagga tgagtcaaac
    15781 acctgggcag atacatccag ttcagtgcgc acatccacat tgccaccgca actctctccg
    15841 ctccattccg ccattatctg gagaatatta tcgagtgcat cttcgaggga gttcgccatt
    15901 gtataaagcg gcgagtgttc ctgcatccgc tcttcattag tctgatcaac agatttggtg
    15961 gatgtatttt cagcccgcag aagtttagcg ccggcatgac gcatctgatt ttccagcttc
    16021 tcaagtgatg tttcgccaga ttctatcgct gcgccactat gttcaacata ttcgaggcca
    16081 ttttttgttc tgtcctcaaa aatcgtagcg gtggatgcac caaccgtcag ttcttcattc
    16141 ctgtccagcc cgtaggccac cagcaatgga acgcgggcaa catgaagaat attgtcctgc
    16201 tcgctctggc tttgccagtg cttgatattc agcaagccaa gattaagcaa tggcggtgta
    16261 ccacgcataa accctgtttt cttcgtatac agtgttacca gaggaatatc atcacggctg
    16321 gtattccatg actcatgaag catccagaca gattcgccat tagtaccttc gctgcgtcga
    16381 taaatttcta ctcgacgggg cataatatgg cggatctgct ccaccttctt ctgcccgaaa
    16441 tcatcaccat caataatgat gacctctttt atacgcaaat cagtgagaac aactttccct
    16501 ttttcaactt tcgatttcca tccaataacc tggcgtggat tcagcatcgt aacgtacggg
    16561 cgaccaccgg ccgcgttttc atcggctttt gtccgaatct cgttcatatc cgttcgtgga
    16621 tagtccacca gcgcatgtgc cacaccatac tgaaatgcga gactgaaaaa ttgctgcgcc
    16681 cacacatcca gtcggctccc ctccatgtcg atattttctg catattccct gattttttcc
    16741 ggcgtttcct cactcaatac tgtcggctct gcaaatatgc gcccaatatt ttgtttgatg
    16801 ctttcttcat acacaggaag tagcgtagcc acagacaggc gttttttata agcgtcttca
    16861 tcttcattag gccatttggg gagataattt tccccctgcc tgcgcatttc aagcgtaccg
    16921 cccatcaatg cgtcgttaat atcccacgcc tccagcatat cgttatagtc gaggttgggt
    16981 gttgatatat cagccataat taaatccgaa gtgatgtgac tcttccggtc ggtttgacaa
    17041 tagggaattg cttaacgatg aaataacctc cggcatcatt cgggtgatca ttgccagatt
    17101 ttttatcagg ctccccctta tcatcccaga cctgttgctc cagagattcg gcatataccg
    17161 gacaacgctt cacattaact ttatagcgac gctcaccatt ggcattgcag aacattgcat
    17221 tcattgagtt aacgcgatct tttactggag ggttcgagct gttcaccaca acgttaaaac
    17281 ctgcctgctt aagctgggct atatccgtcg tacttgcgtt acttgatttt ctggaatctc
    17341 cggaagcatc tggataaata taaatctccc tcactttccg gtaatcatgc ccgtcataca
    17401 gccagaagcg ttctttaatg aggcggatca tatccggcgt atcgtaggca ttgatgattt
    17461 cagttaccgc acatggaagc cccaaacgca gcacatggac gattcccgcc atctttccaa
    17521 cgttaaaatc catcccaata taaatcggct cccctggctg ctccacttct tcgcaattat
    17581 tcagtttccg gtcaaactga tggtaaacag taccacttgt caggttagta aaccgtccta
    17641 gaagataggc tttaatcagc tctggagggt atgattcaag aagtgaagga atgtaatctg
    17701 ctggcaggtt cttttcatta tcgaaagtag atgcctgcac cagaccatac agtgaggcca
    17761 gctctgtttt ttcacgcacg gctttaacaa actgctcgta gacaaatttg aatccttccg
    17821 gcgtggttgt aacgtcaata ccgttgcgaa gtccatcaat cttataacgc atacgcgcaa
    17881 ttatcttgcg ccacgccgtt ctggcttttt ccttcggcaa aatgtccagt tcatccacca
    17941 gcgcattacc aattttgaaa ccgacgatcg tttgtggctt ctccatcgat ctgcagatag
    18001 tggttccccg atactggcgt ccgtaataaa agtgaacctc tttatttccc tcattaattt
    18061 ttacgttcaa tccccagtca gcagcaactt cttccactgt agggtaaaaa atatcgcgaa
    18121 tttggggata cgttggcgca aaatatccct gatttatact tggatgctcc caaatcccct
    18181 tgcatatgcc gccacaccca acccatgttt tgcccgagcc aaaaccagca atataggctt
    18241 taaatttatg gggcattgaa agaaatcgcg cctgaggcac attaagcgtc ggagagatca
    18301 tcttcatcac tccttaatct ggcatcaacc acattaatat tgatcgccac aggctgggga
    18361 tgttcattat cctccaccgt ttcgatctct ttgcgcagct tctggttttc tattctgcgc
    18421 cgttcaattt ccagttcctg tagccgctta tctgcacata atgccccgcc agcagaaagc
    18481 aaacgcaaca attcacgccg ggcggcagcc ttatcctcca gcaggatctc aacaccgtat
    18541 tttccgagct ttgaccctgc atataattgc cgcgcatccc catcaagcag tgtggtatca
    18601 gccatataaa gctgtcccgt tccctcaccg cagcacttcg ggcaatcagg attgggtatg
    18661 gcgttatcaa caaagccgag gcctccatat tccggctcgg gtttaccatc tctggaggcc
    18721 tgtgccgcag ccttatcgaa ttctgcaata tcacgccact ggtagagatg attctcgccc
    18781 cagcaataac ggcagttaac acggcgaaat tgtgccaact gattggggtc ggcctggaca
    18841 atggccatca actggctcac cagtaaatcc aggtctgcgg tatagcgttt ctggtactga
    18901 ttgcggaagt agctgatagc acgataaacc ctggcatttc taagcatacg gctggcgttg
    18961 ctgttagctg tcgcaccttg tccctcataa ccagccagtc ggtatgcctc tgtcggcttt
    19021 ttcccctgag caaccaacat ggcgaatttt gcctgctggt cagaaatacc gaattcatcg
    19081 gggcagaacg aaatttcttc cgtgtccccc tcaatcagga gtgcatcgga tacagccttt
    19141 tttttctgag attttccgtt ctgcctttgc gcagtctgcg cagttttttt tcgcgcactt
    19201 ttttgcgcag ttttgcgcat ttctttctgc gcatttttcg gagttttttt gatgtaacga
    19261 cgggctgttg cgtagttcag tccccttgct tcacaccatg ccaccggaga tataccggag
    19321 cgggtgtatt cagcaatata ctcctgctgc aacgcccccc agtccggtct gctcatcagt
    19381 tagtcctgat ttttatccac cctgagtagt tcgcgcagag caaaggcatc cccttttctg
    19441 gcaagcttaa acaatgccgc tcgtaactcg gcttcacctt tcgctctgcc cttacggatg
    19501 gccgcataaa aatctgtcat tgcttcccga ttttctttca gtcggttcag atcaacatcc
    19561 agaacgtcag cgatttgttg tgcagtcatc cggcacgctg ccagagactc gactttcgaa
    19621 tacggaatca tttgtcaccc ccattgatat gcagggtgtc ttcttcctgt atttttcgtg
    19681 aaggattttt actgcagcgt tgttccaggt gacctgatgg tgaatgcgtt tatggctggc
    19741 acccatcagt gagattttta cgcacgacgg cgaatacatg acggagtaaa aacttttaac
    19801 gtaggttccg gaatccagat acagctcggt cattccgccg ctgtttttct gcgtctgttt
    19861 ctgccctaac tggacagcac cgatcgtcat aaacaattca ccacagcgac cgagattcgt
    19921 gtacgtattc acatcctcgt taatgcgccc catgaatgag aacggtcgat caaccgaaca
    19981 gataaagctg ttcattgcct tgcgtttcac ccacgaagca tggccgccat tgtcaccaag
    20041 aaaatccccg ccctgcgaca tagcgatgga aagcgcgggt attgattcgt aatacgccag
    20101 catttcagaa aggatcgcat ccagtttcct tatcgggaaa taggcctggt catagttgcg
    20161 atccacccga aactggaact cgtgataatc atcatcgagc tgaatgaagt atttacaccc
    20221 gaccagtttt gccaggtcga aacaggcatt acgggcgtaa aaaattgagc ggcggtcacc
    20281 aaaattatcg gcttcgtcaa aacgactagc aatatcggct ttggaaaata ccagcacctg
    20341 ttcaccaaat tcagccatgt actgatgccg tgtcttatct tcatcatcaa caacgataaa
    20401 aattttcccg gtatagccag cacgacgcaa cgtccggtaa gtcagaactt tgtccggtcg
    20461 cccgtgagtc agaataaagg cgcaaaaatc atcacgcata ttcctcctcc tcctccccgc
    20521 catgcatgat ctccaccatg cgctgcgtca ttcggacaaa tccattttca atagcctgct
    20581 gataatcaat gatcaccagc gccgactcct cgaaaaggca ctgaatttca gcgggggcat
    20641 gagcgtaata gtccgcaatt ctgctgaaat tgaacaccgt gtgacgttct gccgcacaca
    20701 ggaggaattt ctcaatatca ggatcaaggg acgccgaacg tatccggctg atcagctcct
    20761 gagttttcgt atcgtcgtac agttcacgga tatccggttt accgcccgac ggctcataaa
    20821 caggcgtatc aattttcgtc gtatacggct cctccttatt tcctgtaccg gacaaaacat
    20881 ccgtcaacag ttcatcaatt tctgtcgggc cgaagcctgt cagggagaca tcaaaatcag
    20941 cattgattag gtccgacagc tccatccgca acagatcttc atcccagcca gcattcatcg
    21001 gcaggcgatt atctgccagg cggtacgcct ttttctgctc atccgtcagg ccagacagaa
    21061 caatgaccgg aacagaatcc attttgagca tttcagccgc cataacacga ccgtgacccg
    21121 caataatttc gcccttttcg tcaatcagca ccggattagt ccagccgaat tgcttaatac
    21181 tttctaccag ttgtgccacc tgctcagtac tgtgcgtcct ggcgttgtgc gcatacggtg
    21241 acaattcttg taatgggcga tagactatct ttaatttctc gctcatacag ccttgcttta
    21301 tgaataaaac gcaccccagc agccagtgct actgggggcg gaggtgttgc tggtaaagtt
    21361 aggtattgga tcaatgagtg agtcaacata atattaaact cacaattata aatcagccat
    21421 atattaggag cgccaaaaaa aacctgaaaa taatataata acaggataaa tttcaaggcg
    21481 accaagaatc atagctatgc acattaaata ttttgcaatg tcattaagca ctccgaatga
    21541 cgatgcagta gccccaaaac ctaatcccat attattaata catgcagcca ctgttgcaaa
    21601 tgatgtaaga aaatcatatc ccataccatt taacaccagt ataaaaaaca ccgtgaagag
    21661 agtataaaga aaaaagaaac tccatacaga cctcattaca cgatctgtaa ctatcttccc
    21721 tcctacattt acactcaaca acgctctggg atgagaaagc tgatttatct cgtgtttgct
    21781 ttgtttgaaa agtataagaa atcgaagtga cttaattcca ccacaagttg aacctataca
    21841 tcccccaaag aaacttgaca acagcaaaaa cactatcgtg tgcgtgggcc agtttgcata
    21901 atcttgcgta gctaaaccat tatcagtgag catggagctg gcaagaaaaa acgaatgaat
    21961 aaaacttcca tgcaagtcat acatacctat atgccagacc tggaaagagg taacaatgat
    22021 caccactaag gctattaaca gaaagaaacg aagttcaata tctctgatta aaggttttat
    22081 cgttttcctg ctaataacaa tataccaaag agtgaagttg aaagccgata gcagggaaaa
    22141 agaaccagcc accagctcaa ccaaatagtt attaaaatat ccgatactct cgctatgagt
    22201 tgagaaacca ccaagcgaaa ctgtggaaat cccgtgacaa atagcatcaa acaaaggcat
    22261 tcctgcaagt ctataacaga caatacaagc aatacctaat aaagaataag ttatccacag
    22321 tgtccgtgac gtatcggcta ggcggggagt gagtttgtca tccttaaacg gccccggcat
    22381 ttctgactga taaagctttg caccaccaat acccaataat ggcaatacag caaccgccag
    22441 aacaataact cctaaaccac ctataaaatt taactgtgac cgatagtaca aatatgcccg
    22501 aggtaatgaa ctaacatcat caattacagt tgctcctgtt gttgttattc cagaaacccc
    22561 ttcaaaaaga gcatcaatga acgttaaatt aagttctgag tcaatccata aagggaatgc
    22621 actaataaca gaaaacaaaa tccaaaacat tacaattata ataaacccat cacgggtacg
    22681 taattgaatg ccagatttct tagttgtata ccacgctccg ccaccaatgc aaaaaaatat
    22741 aacgaaggtt ataaagaaaa cgaacaggct tttttcttta taaaacaatg ctacaaccat
    22801 tggtggcaac attgaaagac tatagagcca aaccaggaac ccacacatat gagtaacaac
    22861 tcttacatga gatgtattca tatctaaata ttctttcaat tataaccacc ttgctgcaat
    22921 attatgatta tactgtataa aatttaactc ctcttagatc ttacttcact gttccttatg
    22981 aaacaatcat caaaataaat catattgtag ttaagatttt actttaaaca ctgttcggtt
    23041 atgtattgct gagctccttc aagttgggcc tgcatcatta ccagtcgttc ccggagggtg
    23101 aaataatccc gttcagcggt gtctgccagt cggggggatg ctgcattatc cacgccggag
    23161 gc
//
LOCUS       DAJOWB010000071        22989 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJOWB010000071 DAJOWB010000000
VERSION     DAJOWB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.071"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..2956
                     /gene="rne"
                     /locus_tag="OHO85_002967"
     CDS             <1..2956
                     /gene="rne"
                     /locus_tag="OHO85_002967"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCQ2672314.1"
                     /translation="FPANYSAHGRPNIKDVLREGQEVIVQIDKEERGNKGAALTTFIS
                     LAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLELPEGMGLIVRTAGVGKSAEA
                     LQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAFRDYLRQDIGEILIDNPKVL
                     ELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIESAFQREVRLPSGGSIVIDS
                     TEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQLRLRDLGGLIVIDFIDMTPV
                     RHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRLSPSLGESSHHVCPRCSGTG
                     TVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASYLLNEKRSAVNAIETRQDGV
                     RCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEEAMALPSEEEFAERKRPEQP
                     ALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPAQPGLLSRFFGALKALFSGS
                     EETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRDTRSERTEGSDNREENRRNR
                     RQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRRRNDDKRQAQQEAKALNVEE
                     QSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAVVAPVVEETAAAEPIVQEAP
                     APRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRSRRSPRHLRVSGQRRRRYRD
                     ERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQVEEQREQEEVQVQPMVTEVP
                     VAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTHPEVIAAAVTEQPQVITESN
                     VAVAQKVAEHAEPVVEPQEETADIEEVAETAEVVVAEPEVVAQPAAPVVAEVAAEVET
                     VAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQRPTFAFEGKGAAGGHTATHH
                     ASAAPARPQPVE"
     gene            complement(3153..4106)
                     /gene="flgL"
                     /locus_tag="OHO85_002968"
     CDS             complement(3153..4106)
                     /gene="flgL"
                     /locus_tag="OHO85_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCQ2672315.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGFRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(4118..5761)
                     /gene="flgK"
                     /locus_tag="OHO85_002969"
     CDS             complement(4118..5761)
                     /gene="flgK"
                     /locus_tag="OHO85_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCQ2672316.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNTGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(5827..6768)
                     /gene="flgJ"
                     /locus_tag="OHO85_002970"
     CDS             complement(5827..6768)
                     /gene="flgJ"
                     /locus_tag="OHO85_002970"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCQ2672317.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEEPTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(6768..7865)
                     /gene="flgI"
                     /locus_tag="OHO85_002971"
     CDS             complement(6768..7865)
                     /gene="flgI"
                     /locus_tag="OHO85_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCQ2672318.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(7877..8575)
                     /gene="flgH"
                     /locus_tag="OHO85_002972"
     CDS             complement(7877..8575)
                     /gene="flgH"
                     /locus_tag="OHO85_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCQ2672319.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(8628..9410)
                     /gene="flgG"
                     /locus_tag="OHO85_002973"
     CDS             complement(8628..9410)
                     /gene="flgG"
                     /locus_tag="OHO85_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCQ2672320.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(9582..10337)
                     /gene="flgF"
                     /locus_tag="OHO85_002974"
     CDS             complement(9582..10337)
                     /gene="flgF"
                     /locus_tag="OHO85_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCQ2672321.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(10357..11562)
                     /gene="flgE"
                     /locus_tag="OHO85_002975"
     CDS             complement(10357..11562)
                     /gene="flgE"
                     /locus_tag="OHO85_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCQ2672322.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(11587..12282)
                     /gene="flgD"
                     /locus_tag="OHO85_002976"
     CDS             complement(11587..12282)
                     /gene="flgD"
                     /locus_tag="OHO85_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCQ2672323.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(12294..12698)
                     /gene="flgC"
                     /locus_tag="OHO85_002977"
     CDS             complement(12294..12698)
                     /gene="flgC"
                     /locus_tag="OHO85_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCQ2672324.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(12702..13118)
                     /gene="flgB"
                     /locus_tag="OHO85_002978"
     CDS             complement(12702..13118)
                     /gene="flgB"
                     /locus_tag="OHO85_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCQ2672325.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            13273..13932
                     /gene="flgA"
                     /locus_tag="OHO85_002979"
     CDS             13273..13932
                     /gene="flgA"
                     /locus_tag="OHO85_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCQ2672326.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSAQLAGISDEVR
                     VSIRTAPSLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            14008..14301
                     /gene="flgM"
                     /locus_tag="OHO85_002980"
     CDS             14008..14301
                     /gene="flgM"
                     /locus_tag="OHO85_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCQ2672327.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            14306..14722
                     /gene="flgN"
                     /locus_tag="OHO85_002981"
     CDS             14306..14722
                     /gene="flgN"
                     /locus_tag="OHO85_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCQ2672328.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSADISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(14762..16297)
                     /gene="murJ"
                     /locus_tag="OHO85_002982"
     CDS             complement(14762..16297)
                     /gene="murJ"
                     /locus_tag="OHO85_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCQ2672329.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGVLQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(16407..17330)
                     /locus_tag="OHO85_002983"
     CDS             complement(16407..17330)
                     /locus_tag="OHO85_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCQ2672330.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(17332..17979)
                     /locus_tag="OHO85_002984"
     CDS             complement(17332..17979)
                     /locus_tag="OHO85_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCQ2672331.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPSVTDMSSAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(17990..18574)
                     /gene="rimJ"
                     /locus_tag="OHO85_002985"
     CDS             complement(17990..18574)
                     /gene="rimJ"
                     /locus_tag="OHO85_002985"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCQ2672332.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            18810..20018
                     /gene="mdtH"
                     /locus_tag="OHO85_002986"
     CDS             18810..20018
                     /gene="mdtH"
                     /locus_tag="OHO85_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCQ2672333.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            20082..20729
                     /gene="grxB"
                     /locus_tag="OHO85_002987"
     CDS             20082..20729
                     /gene="grxB"
                     /locus_tag="OHO85_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCQ2672334.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            20863..21423
                     /locus_tag="OHO85_002988"
     CDS             20863..21423
                     /locus_tag="OHO85_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCQ2672335.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGG
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            21529..22575
                     /gene="pyrC"
                     /locus_tag="OHO85_002989"
     CDS             21529..22575
                     /gene="pyrC"
                     /locus_tag="OHO85_002989"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCQ2672336.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            22649..22894
                     /gene="dinI"
                     /locus_tag="OHO85_002990"
     CDS             22649..22894
                     /gene="dinI"
                     /locus_tag="OHO85_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCQ2672337.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT         5407 a         6228 c         5907 g         5447 t
ORIGIN      
        1 tttccctgct aactacagtg ctcatggtcg tcccaacatt aaagatgtgt tgcgtgaagg
       61 tcaggaagtc attgttcaga tcgataaaga agagcgcggc aacaaaggcg cggcattaac
      121 cacctttatc agtctggcgg gtagctatct ggttctgatg ccgaacaacc cgcgcgcggg
      181 tggcatttct cgccgtatcg aaggcgacga ccgtaccgaa ttaaaagaag cactggcgag
      241 ccttgaactg ccggaaggca tgggacttat cgtgcgcacc gctggcgtcg gcaaatctgc
      301 tgaggcgctg caatgggatt taagcttccg tctgaaacac tgggaagcca tcaaaaaagc
      361 cgctgaaagc cgcccggctc cgttcctgat tcatcaggag agcaacgtaa tcgttcgcgc
      421 attccgcgat tacttacgtc aggacatcgg cgaaatcctt atcgataacc cgaaagtgct
      481 cgaactggca cgtcagcata tcgctgcatt aggtcgcccg gatttcagca gcaaaatcaa
      541 actgtacacc ggcgagatcc cgctgttcag ccactaccag atcgagtcgc agatcgaatc
      601 cgccttccag cgtgaagtcc gtctgccgtc cggtggttcc attgttatcg acagcaccga
      661 agcgttaacc gccatcgaca tcaactccgc acgcgcgacc cgcggcggcg atatcgaaga
      721 aactgcgttt aacaccaacc tcgaagctgc cgatgagatt gctcgtcagc tgcgcctgcg
      781 tgacctcggc ggcctgattg ttatcgactt catcgacatg acgcctgtac gccaccagcg
      841 tgcggtagaa aaccgtctgc gtgaagcggt gcgtcaggac cgtgcgcgta ttcaaatcag
      901 ccatatttct cgctttggcc tgctggaaat gtcccgtcag cgcctgagcc catcactggg
      961 tgaatccagt catcacgttt gtccgcgttg ttctggtact ggcaccgtgc gtgacaacga
     1021 atcgctgtcg ctctctattc tgcgtctgat cgaagaagaa gcgctgaaag agaacaccca
     1081 ggaagttcac gccattgttc ctgtgccaat cgcttcttac ctgctgaatg aaaaacgttc
     1141 tgcggtaaat gccattgaaa ctcgtcagga cggtgtgcgc tgtgtaattg tgccaaacga
     1201 tcagatggaa accccgcact accacgtgct gcgcgtgcgt aaaggggaag aaaccccaac
     1261 cttaagctac atgctgccga agctgcatga agaagcgatg gcgctgccgt ctgaagaaga
     1321 gttcgctgaa cgtaagcgtc cggaacaacc tgcgctggca acctttgcca tgccggatgt
     1381 gccgccagcg ccaacgccag ctgaacctgc cgcgcctgtt gtagccccag caccgaaagc
     1441 tgcaccggca acaccagcaa ctcctgcaca acctgggctg ttgagccgct tcttcggcgc
     1501 actgaaagcg ctgttcagcg gtagtgaaga aaccaaaccg accgagcaac cagcaccgaa
     1561 agcagaagcg aaaccggaac gtcaacagga tcgtcgcaag cctcgtcaga acaaccgccg
     1621 tgaccgtaat gagcgccgcg acacccgtag tgaacgtact gaaggcagcg ataatcgcga
     1681 agaaaaccgt cgtaatcgtc gccaggcaca gcagcagact gccgagacgc gtgagagccg
     1741 tcagcaagct gaggtaacgg aaaaagcacg taccaccgac gagcagcaag cgccgcgtcg
     1801 tgaacgtagc cgccgccgta acgatgataa acgtcaggcg caacaagaag cgaaggcgct
     1861 gaatgttgaa gagcaatctg ttcaggaaac cgaacaggaa gaacgtgtac gtccggttca
     1921 gccgcgtcgt aaacagcgtc agctcaatca gaaagtgcgt tacgagcaaa gcgtagccga
     1981 agaagcggta gtcgcaccgg tggttgaaga aactgccgct gccgaaccaa ttgttcagga
     2041 agctccagct ccacgcacag aactggtgaa agtcccgctg ccagtcgtag cgcaaactgc
     2101 accagaacag caagaagaga acaatgctga taaccgtgac aacggtggca tgccgcgtcg
     2161 ttctcgccgc tcgcctcgtc acctgcgcgt gagtggtcag cgtcgtcgtc gctatcgtga
     2221 cgagcgttat ccaacccagt cgccaatgcc gttgaccgta gcgtgcgcgt ctccggaact
     2281 ggcctctggc aaagtctgga tccgctatcc aattgtacgt ccgcaagatg tacaggttga
     2341 agagcagcgc gaacaggaag aagtacaagt gcagccgatg gtgactgagg tccctgtcgc
     2401 cgccgctgtc gaacctgttg ttagcgcgcc agttgttgaa gaaatggctg aagtcgtaga
     2461 agcccccgta ccggttgccg aaccgcaacc ggaagtggtt gaaacgacgc atcctgaagt
     2521 gattgccgcc gcggtaactg aacagccgca ggtgattacc gagtctaatg ttgccgtagc
     2581 ccagaaagtc gcagaacacg cagaaccggt ggttgaaccg caggaagaga cggcagatat
     2641 tgaagaagtt gccgaaactg ctgaggttgt ggttgctgaa cctgaagttg ttgctcaacc
     2701 tgccgcgcca gtcgtcgctg aagtcgcagc agaagttgaa acggtagccg cggttgaacc
     2761 agagatcacc gttgagcata accacgctac cgcgccaatg acgcgcgctc cggcaccgga
     2821 atatgttccg gaggcaccgc gtcacagtga ctggcagcgc cctacttttg ccttcgaagg
     2881 taaaggtgcc gctggtggtc atacggcaac gcatcatgcc tctgccgctc ctgcgcgtcc
     2941 gcaacctgtt gagtaataat tagctcaaag taatcaagcc ctggtaactg ccagggcttt
     3001 tttatttcat gtttgaatca acatattaaa attcatcaaa tggcatcctt gctaaccaac
     3061 aatgcaaaat aggcaaaaaa aatgcccgcc ccggcgatcc cagagcgggc agacaaaaca
     3121 tacccagttt catgatatgt tttaaagcga gcttatttgt tgagctggaa gagcgacaat
     3181 ccctgcatat cggtaaatgc tttatacgat gcctgcaatg ccgtttgctg catgatgtaa
     3241 gatgaaatag ttgcattcca gtccacatca accagatcgc tcatctgctg cgtttgccct
     3301 aaagcgcgat cgctacctaa ggaatccagc gactccagtt cgttcagctg cgtgcctaat
     3361 tccgcgcgaa cggtaaggac attattcagc gagtttttca gtccacggtt ggttttatcc
     3421 aatgccgcag cggcgatttc tttatccgct tcgctatccg cgaccggcgt tttcagggct
     3481 gcgatggcgc tatccagcat ggcaaaaaga ttggtttcag aagcgctacc gtctggttcc
     3541 gctaccgcgt tactggtaat actgtcgaaa attttgtcgc ccgtatgtcc tatcaccatc
     3601 gaacgcgaag catcgacctg ttgcttaata ctttctgttc cgccgacata atcaccgtta
     3661 gcctcactaa acggcgcagt ctctgtttta taaccggcaa aaatgtagcg cccattaccg
     3721 tcagtggtgt tcgccagatt cagcaactgg tcacgaaacc cctgaatatc cgtagccagc
     3781 gaggcccggt catcgtcact caaggtgcca ttgctggcgt agacaatttt ttcctgagca
     3841 ttctggatag cagtggtgac ctggctaagt acactctctt ccagtgacac tttttgagtg
     3901 gcgaaagtac gtgccagcgt gtactggctg ttttgcgcct gtgcctggga gagaacaaca
     3961 gcttgtgatg cagcaatggg atcgtctgaa gggttaacga ctcgcttacc cgtcgacatc
     4021 tgctcgccgt acttcatcca ttctgcctga gaattggtga tgccgcgcat gttttgctgg
     4081 tacatcatct gggtactgaa acgcatttta tctcccctta gcgaatgtta atcagcgcat
     4141 caaaaatcgc gtttgccgtc tgcagaacct gcgcattcgc caggtaatac tgctgaaaac
     4201 gttgcagatt tccgtactcc tcatcgagat tgacaccgga aatcgactgc tgctgattgg
     4261 aaagctgcgt caccacatta ccttgcgtgg cgctactggt tttcaacgtc gcggttttat
     4321 taccgatatc actcactaac gaggcataag cgtcgttaaa ggatttcgca ccgcccaccg
     4381 ttttactgtt gctttgcaga tccagcaggg cctgaccgtt gcggttatcg ctatcacccg
     4441 catcttcttc gctcgccatc gctattttcg cttcgtcggt gattaataca tccatgttga
     4501 cgatagcgtc acttactggt ttcagcgtga agctgtcgtt aacagcaggc gttcctgtaa
     4561 acgtcaactc cagaccatca aatgccactt taccgttggc atccggcgtc accgtaaaag
     4621 tggtattact ggcaaggcgg gtgacctgcc actgattatt atcgaacgag attttgtaat
     4681 cagtcgccag taccgcggag gcatcagtta ccgtggcacc gatcgcaacg tcacctgtgt
     4741 ttttcgtgtt ttgcagaacc gcgggcttac cgatagcaaa gaaatcttca ccagcatcgc
     4801 cgttggcatc aaatccggct ttgtgttggc tgttgaaagc ctcggcaaat gccagcgcca
     4861 gttgtccaag cgtattacgc gtctggtcca gatcctgaga acggaatgtc agaatgccgc
     4921 ccagcgaccc ggtattcagt aatttctccg ggatctcaat attgcctgcc gtcccatcaa
     4981 cgtaagcgac agtcgtacga gaagggtcgg cgctggaagg aactgccgcc agttgccgcg
     5041 ccgtacttcc ctgaaccagt gagtaaccat tggccatcgt gatgttataa gtaccgccat
     5101 cctgaacgct gacttctacg ccaacaatct ggtttaattc gctcaccagt tgatcgcgtt
     5161 gatccagcag attgttaggt gacgcccctg cccccacgcc tgtcaggcgc gagatttgat
     5221 cgttcaggct ggcaatttgt ttagcgtaat tgttgatctg atcaacgctg gcacctatcg
     5281 cgatattgac ctgtttgtcc tggtcgcgca gatattgatc ggtggtttta aactgattca
     5341 ccaacccttc tgatttccca atcagcgcct ggcgcgctgc cgggtcttcc gcgttactca
     5401 ccagcgtttg caggctggtg aagaaatcct gcatctgtgt tgccagcgaa gaggtactgg
     5461 tggagagcat attgtcgatt ttcgacatct gctcatagcg ggcagtcagg ccgctacttt
     5521 gcgtctgcgc cgcacgtaac tggttggtaa taaaagcatc atactcacgc tgcacaccag
     5581 aaacgtagac accattgcca acccagccgc cagcgcccaa cgtgctattg gcctgcgcca
     5641 taatagtggt ttggcgggta tatccggcaa cgttatagct ggagatatta ttacttgccg
     5701 tatttaacgc cgcctgggcc gcgttcagtc cgctcatggc gttattgatc aagctggaca
     5761 tggaggttcc ttataagcct tcaattacag agtattatcg gcagcgaccc gccggacttg
     5821 agttattcag aacagattat caatgttcat gctgtaggtt ttgctcacct tgtcgcttat
     5881 cgatttcatc tgctggatca tgttggtgag tttacgggca tagtgaggat cggtagcata
     5941 ccccgcgtcc tgtagggcct gcgccccctg ctccgcactc gcggcggtcg tcacggcggc
     6001 gtagcgcggg ttacgtgtta acaacccaac gtaatccgac aatgcttcca gatacgagct
     6061 gtagacccga aatttcgctt ttactttctt cgcttcgcca ttttcatatt cagtcgtggt
     6121 gatttcagtg actggccctt tccagttgcc agaggctttg acaccaaaca ggttatagct
     6181 cggctcgccg ttttcgcggc ggatttgtcg ttgcccccag ccagattcca gcgccgcctg
     6241 agcgaggatc aaatgatgtg gcacaccgct ttgctggctt gccagttggg cgggcagcga
     6301 gagttgcgcg aggaatgctt tactgtcacc cggcagcgaa tcatcgtagt tacgtggtac
     6361 ggccttttgc accagctgag aaagcgcctg attttgataa cgcaccacag tttcgagcgg
     6421 gaatttcatc ggtgctgctg gcgtgggctc ctctggcaat ggttgttctg gcgtcatctg
     6481 tttaaccatc atctctgcaa gccccagacc tttgcccgcc gtcatctgtt gggcaatctg
     6541 ctggtcatac atactggtat acaggcgagt gtgctcgctg ctgaacaggc catcttttgg
     6601 taaagcgtcg cgcatgcttt tcaacatcat ctgcacgaac atcccttcca cctggcgggc
     6661 caccggacgg atatttgccg ccggatcttc gcccgctttc gcctttagtt cgttgagtga
     6721 ttgcgcatcc caggccgcac ttgccagtag tttgctgtcg ctgatcatca gatgatttcc
     6781 agttttgccc gcagacatcc cgcactttgc attgattgca aaatagacat cagatccatc
     6841 ggcgtagcgc ccagcgcatt gagcgcgcgc accacgttat tgaggctggc gctggaacgt
     6901 acgctttgca gcgaaccgcc gctctggcgt aagtcgatct gcgtttgtgg cgttaccacg
     6961 gtctgtccgc caccaaacgg tgtatctggc tggctgacat tggcctgacg attgactgta
     7021 actgagagat tcccctgcgc taccgcgcag ctgtcgaggg tcacttcgcg attcatcacc
     7081 accgaaccgg tgcgcgagtt aatcactact ttagcgtcct gcggggtgac attaacctgc
     7141 atattctgga tatcggcaag gaaacggacc tgggaactgt tgccacttgg tacgcgcacc
     7201 tgaatagtcc gcgcatctaa cgcggtggcg ctgccatatc cacgcacgcg gttgatggtg
     7261 tcagcgattt gctgcgccat gctgaaatct tcgtcgttaa gttgcaaatt aagggtattc
     7321 ccgacgccaa actggctggg caattcacgt tcaataaccg caccattggt gatccgtcca
     7381 ccgttcagtt ggttcacctg aacactgcta ccgccagcgg aggctcctgc gccgccaacc
     7441 agaatattgc cctgcgccag cgcatacacc tgactgtcaa cgcccttaag cggtgtcatc
     7501 aacaacgtac ctccacgcaa gcttttggca ttacccatgg aggaaaccac cacatcgatg
     7561 gtttgcccct gacgtccaaa cggaggaagt gacgctgtca ccattaccgc agcgacgttt
     7621 tttagctgca tattggtgcc cgtcggaacg gtaattccca gctgtgagag catgttatta
     7681 agcgtttgtg tggtaaacgg cgtctgggtc gtctggtcac cggttccatc cagccccacc
     7741 accaggccat agccaatcag tgagttttgc cttaccccct gaacactggt gagatcgcga
     7801 atacgctcag cctgagccgc cgtcgtaacc agtagaagaa ttaatgcaga gagaaattta
     7861 atcaccacag cctcacttac attggcgaca ggttaaggaa gaaacgttgc aaccagccca
     7921 tattttgcgc ttcgttaatg tagccattgc ctacgtattc aatgcgcgca tccgccacct
     7981 gagtagacgg tacggtattg ctgccgctga tggtgcgtgg attaaccacg ccagagaagc
     8041 gaataaattc ggtaccctga ttaatggcaa tctgtttttc acccaccaca tgcaggttgc
     8101 cgttgaccag tacctggtca accgtcaccg tcaacgtgcc gctaaaggta ttgctggcat
     8161 tggccccgcc ttttccgttg aacgtgttac caccggaggc ttcgacatcg gcacgagcgt
     8221 taccaaacag cccctgcaaa tagcgcggca cagtatcaaa gccaaaatta gttttaccgt
     8281 cacggctggc attcgcagag gagcttttgc tggcgctgac gttctcctgc aacacgatgg
     8341 tcagcgtatc gccaatattg cgtggtcgac gatcttcaaa cagcggttga tagccatagt
     8401 taatcggctg agcagactgg aaaatagaac cgttggcgac gggagtcgga ccgggaaccg
     8461 gttgtgcact ggtcgctccc tgtaccagcg gcgtggaggg tatccaggcg cagccggtta
     8521 gtgaaagcac caacaagctg gaaatggcat aagtatgcgc agcgtttttt tgcatggcta
     8581 tcatcttcaa aaatcaataa accggtgaac ctgccaccgg ttatgcctta gagttgcgtc
     8641 agtttttgca gcatctgatc ggtggtggac accgctttac tgttgatttc gtaagcgcgt
     8701 tgcacctgaa tcatattgac cagttcttcc gccacgttga cgttagacgt ttcaacatac
     8761 ccttgataca gcagtcccgc accgttcagg cccggcgtgc tttcgttcgg tgcaccggag
     8821 gattgcgttt cggtgtagag gttttcgcca atgctctcca gtccggtatc attcataaag
     8881 gtggtgagat tgagctgccc aacctggacc ggagctgcct ggccttgttg ggttacgctg
     8941 accacgccat cacgaccgat ggtgatactt aacgcattcg ccggaatggt gatcgctggc
     9001 tgcacctgaa aaccaccagc cgtcaccaac tgcccgttct gatccacctg gaaagagccg
     9061 tcacgggtat aggctgatga gccatctggc aacatcactt ggaaaaagcc ctgcccttta
     9121 atcgcgacat ctttgctgtt gttggtctgc gacaggttgc cctggctgtg taagcgttca
     9181 gtggcgaccg ggcgtacccc cgtgccgatt tgtaatccgg agggtaaggt ggtttgttcg
     9241 gaagactgtg cccccggctg acgaatggtt tgataaagca gatcttcaaa caccgcgcgc
     9301 tgacgcttaa aaccgttagt actgacgttt gccaggttgt tggcaatgac gtccatattg
     9361 gtttgctggg cgtcaaggcc cgttttggcg atccataatg aactgatcat aaaatgtcct
     9421 gtagtggatt agtgacaaag tgcatatgtc ttcttgccgg atgcggcgta aacgccttat
     9481 ccggcctaca agtgctcgta aaatcgataa attgcgattg cccccataga cccgataaac
     9541 ttgcgcatcg ggcaacttat acttgtcatg tatcctttca attaactcat cgacagcagt
     9601 tggttggcac ggcctgcgtt atcatcgacg ctgctgatca ccttcatctg catttcaaaa
     9661 cgccgcgcgc tggcaatcat gtcgctcatt gccgcaacgg cattgacgtt actgccttcc
     9721 agaacacccg acatcacacg caaggttgga tctgcctgca gtacaggccc acgcgtggcc
     9781 tgggtttctg cgcttaaacg aaaaatgccg tcgtcaccgc gctgcacttc gctgcccgtg
     9841 gctttcacca gcttcagacg ccccactggc gcaaccgtat ttgccggatc gcccggattc
     9901 agcgccgaga ttgtgccatc ggcagctatc gtgatttccg ccccttccgg cacagcaatt
     9961 ggccccgcct cgcctatcac cggatgcccc tgaattgtca gttgcccggt gggatcaacc
    10021 tgaatgctgc cattacgcgt atacccttcg ctgccgtcag cggtctgcac ggccagccaa
    10081 ccatcctgct gcaacgcaac gtccagcggg cgcgaggtgt aatccatctt gccgggcgtc
    10141 atgtctgcac ccggtgttga cgccgttacc agcgtgcgcg taggcagtga aagcccttcc
    10201 accggcactg cgcgcaaggc gtttaactgc gcacgaaaac cgggcgtaga ggcattggcc
    10261 agattactgg cggttaccgc ctgttgattc agcgtctggc tggctgctcc catcgcggta
    10321 taaattgcgt gatccattga gctatcccgt cagcgattag cgtaagttaa ccagcgtgtt
    10381 gaggatctgg tcctgggttt taatggtctg ggcgttagac tgatagttac gctgggcaac
    10441 gatcatattg accagttctt tactgagatc gacgttggac gcttccagcg caccgttggt
    10501 cagggtacca aagttgcccg tcccggctgt ccccaacagc gccacgccag aagattgcgt
    10561 cgcagaccag acgttgtcgc cttcggatgc cagaccttcg ttgttggcaa agttcgccag
    10621 tacaatctgc cccagcagtt gggtttgttc gttggaatag ttgccgacaa ccgtaccgtc
    10681 atcattgatt tgataactca ccagatcgcc cggtttgtag ccgttctggg tggttgccac
    10741 aatgttgtta gcgccggtat tttgctgcat ggagttgagg aagctcaggc taaacgtggc
    10801 gggatctgcg ccgttaattg cgccagtggt aatattctct gttggatttg aggtcagaac
    10861 gccattggca ttaaacacca gcttcattgc aggctcggct gtacctgttg gatcactgct
    10921 atcctgggtg tagacctgcc agttattatc cccggtcttc acaaagtaga cgctcatgtc
    10981 atgagcatta ccctgactgt cgaaaacagt caccgaacct tttttgttat agctatccgc
    11041 attgctggcg ctgaatggcg taacagtagg aagcggatca ctggaattca ggttgatctg
    11101 catcgacgcc gtggtggtag ttttcgctgc catcagggta ttcgggatcg aaatgttagt
    11161 cggattcgcc ccttgctgaa tagtcggcgg cgtaccggtt gccgggtaac cggtcagctg
    11221 taaaccttgc atattcacca ggttacggtt ttcatccagc ttaaattgtc cgttacggct
    11281 gtagaacacc gaaccgttgc tgtctaccag acggaaaaaa ccgttctggc tgatagcaac
    11341 gtccagacca cgcccggtgt tggtggtcgt gccatcggta aagtcctgag tgataccggc
    11401 aacttttacc cccagtccca ctttcgaacc ggcaaacata tcggcaaaag aggccgtgcc
    11461 tgatttaaag ccgtatgtgg cggagttggc gatattgttg ccaataacat cgaggttggt
    11521 ggcggcagcg tttaatccgc taaccgcttg agaaaaggcc atgactgact cctgaaatgt
    11581 gaaggcttaa attatctgcc gtacttcgtc gagggtggtg gtgccgtaag tgccgagatc
    11641 cagcgtatta ccgttgttgc cgcggatcac accctgcacc agagcaaact gcagcggctg
    11701 ggcaaccagt tgtgtaccac cgttactggc gctaatcgct acgttgtaag aaccgttcgg
    11761 cgcagtgctg ccgtcagtca acgtaccgtc ccaggtgaaa ctgtggactc cggcggtcag
    11821 ttcaccaata tcaatggtgc gcacaaccgc gccattttta tcggtgatgg tggccgtaac
    11881 tttgtctgcc gcctgttgca gctcaacacc aaacggcgtg gtcgtggtca cagccccttc
    11941 ttcactgccg gttcccgcaa gaacagtggt gccggggatc atcacgccgt gaccaatcag
    12001 gttactggcc tgtaacgact ggctgttatc aatctgtccg gaaatagatc cgagcgtggt
    12061 attgagtttt tcaatcccgc tgaccgtgct gatttgtgcc aattgcgacg tcagctcgtt
    12121 gttttccatt ggattggtcg ggtcctggtt tttcagctgc gccaccagca aagtcagaaa
    12181 actgctttgt aaatctgcgg cgttgctgcc cgtgagcgaa ctactgctgg tggtactgac
    12241 gccggtattt gtcggatcgg tggtggttac cgcaatggac atagctttct cctttattga
    12301 ccgagcgtaa gggttttcag catcatgctt ttcaccgtgt tgagcacttc aacattggcc
    12361 tgatagctgc gtgacgccga catggtgtta accatctccc cgacgacatc aacgtttggc
    12421 attttcacgt agccctttgc atccgccagc ggattacccg gttcataaac cagtttgtcc
    12481 ggggcctgac tttctataac atcggcaacc tttacgccgc ctgtcgcagc acctggtgca
    12541 gcgttaacct ggaataccac ctgttttgcc cgatatggct gtccatcagg accggtcacg
    12601 ctatcagcat tcgccagatt actggccgcc acgttcaggc gctgggactg ggcagttaac
    12661 gccgacccgg cgatatcaaa aatattcagc agtgccatcc gttaatttcc gctctgtaaa
    12721 acgttcatca tgcctttgat ttgcccgctc aacgcgctaa ggctcatctg gtattgcagg
    12781 ctgttatcgg caaactgggt gcgttcgcga tccatatcga cggtattacc gtcaagcgaa
    12841 ggctggtccg gaatacggta ttgcagttct gcggtaggag gcgtcagcgc ctgcgccgga
    12901 atgtgttgcg ttgaggtcat cgtcagtgca accacactgg ttgcatcccg tccacgttgc
    12961 atgacttttt taagttcact ggcaaaatcg atatcgcgcg cctgataacc aggggtatca
    13021 gcattggcga tgtttgctgc cagcacttcc tgacgctgtg cgcgcagatt gagcgcctct
    13081 tgttgaaaac gtaaggcggc gtcgagctta tcgagcatat ctcctccgca ggtatcaaaa
    13141 ttctgccatc agcttaaatg cctttacgcg cgcgttatcg gcggaataaa cgcaaaatgg
    13201 gtcgctattt atgccgttga tgaccattgc ggacaggtac aattcacgtt gtagaaatgg
    13261 ctgggggcga aaatgctggc aataaaacgt agcgtggcga tcatcgcgat actgttcagt
    13321 ccgttaagtg cggcgagtaa tctcacatcg caattgcaaa ccttttttag cgcccagctc
    13381 gcagggataa gtgatgaggt tcgtgtttct attcgtacag cgcccagtct actgccgccg
    13441 tgcgagcagc cattgctttc gatgagcaat aattcccgcc tgtggggcaa tgtgaatgtg
    13501 ttggctcgct gcggtaacga caaacgatat ttacaggtta atgtacaggc cacaggaaat
    13561 tatgtggttg ccgcgatgcc cattgtgcgg ggaggaaagc tggaagctgg caatgtcaaa
    13621 ctgaaacgcg gacggctgga taccctgcca ccgcgtacgg tgctggatat caatcaactt
    13681 gttgatgcca ttagcctgcg cgatctatca cccgatcaac ctatccagtt aacccagttt
    13741 cgccaggcat ggcgggtaaa agcgggacaa cgcgtcaatg tgatcgccag cggtgatggg
    13801 tttagcgcca acgcagaagg tcaggcgctg aacaatgcag ccgtcgcaca gaatgcgcgg
    13861 gtgcgcatgg tatcgggaca ggtagtcagc ggcgttgttg atgcagatgg gaatattctt
    13921 ataaacctgt aagctgttaa agattacccg tcccttgccg ataaataagc aacacatgat
    13981 aaaagcgccc tcaatgagga ataaaccatg agtattgatc gcacttcgcc tctgaagcct
    14041 gtaagcaccg ttcaaccgcg cgaaaccact gacgcgccgg taacgaacag ccgggcggca
    14101 aaaacaaccg cctccaccag caccagtgtg acgttaagcg acgcgcaagc aaaactgatg
    14161 caacccggca gcagtgatat caatcttgaa cgtgtcgaag cgttaaaact ggcgattcgt
    14221 aacggtgaac taaaaatgga caccggcaaa attgccgatg cgctgatcaa cgaagcgcag
    14281 caagacttgc agagtaactg atcgtatgac acgtcttgca gagatcctcg accagatgtc
    14341 cgctgtgctt aacgatctca aaacggtaat ggatcaagaa cagcaacatc tctctatggg
    14401 gcagatcaac ggcagccagt tgcaatggat tacagaacaa aaaagctcac tgctggcgac
    14461 gctggattac ctcgaacagt tacgcaggaa agaacccaat acagcaaata gcgctgatat
    14521 tagtcaacgc tggcaggaaa ttactgtgaa aacgcagcaa ctacgccaaa tgaatcaaca
    14581 taacggctgg ttactggaag gacagattga gcgcaatcaa caggcgctgg aaatgttgaa
    14641 accgcatcag gaaccgacgc tatatggggc gaacggtcag acctcaacaa cccatcgcgg
    14701 cagtaaaaag atttcgatct gaagattatc tccggcctgc actgcaggcc ggaatgcatt
    14761 gttacaccgt ccggcgggca aattctttaa ctttgaagcc cagtaccgcc agtgcagcga
    14821 agtacgcggc aatccccgcc agcacgaccg ccattaaacg cagcaagcgc cagggcatgg
    14881 tacccaatga ccactccggc atgatatgta acatacctaa aagcacgcca gacatcacca
    14941 gtaccgccac caccagacgc aacagaaacg ccatccagcc aggttgcggg gtaaagattt
    15001 tctgcttacg caactgccag taaagcagcg aagcattcag acacgccgcc agaccaatag
    15061 aaagtgacag cccggcatgt ttcaacggac cgataaacgc caggttcatc aattgcgtta
    15121 aaatcagcgt aacgatggca attttcactg gcgttttaat gtcctggcgg gaataaaagc
    15181 caggagccaa cactttcact acaatcaggc caatcaaacc caccgagtag gcaattaacg
    15241 cccgctgggt catcagcgca tcaaacgcgg taaatttacc gtactggaac agcgaaacgg
    15301 tcaacggacc tgaaagaatg cccaacgcaa ccgcactcgg cagcgccaac aggaaacaaa
    15361 gacgcagccc ccagtccatc aaacggttgt attcatcatg attgccactg gcaaaacttt
    15421 tcgacagcga cggcagcaaa atggtgccaa gcgccacgcc cagcacaccg gacggaaact
    15481 ccattaagcg gtcggcgtaa tacatccaag acaccgaacc ggaagcaaga aacgaggcaa
    15541 aaatggtgtt gatgattaag gagatctggc tcacagagac gccaaggatc gccggtccca
    15601 tctgtttcac cacgcgcatt gctccggcat cgtggaagtt aatgcgcggc aggaccagca
    15661 tgccgatctt cttcaggtgc ggtagctgat acaccagctg caggacgccg ccgaccgtaa
    15721 cagcccacgc cagcgccagc accggtgggt taaagtacgg tgcggcaaac agcgcgaaac
    15781 caatcatgct gatgttaagc agtgttggcg caaacgccgg aatcgagaag cggttccacg
    15841 tattcagaat cgctcccacc agcgacgcca gggagatcag caagatatag ggaaaggtaa
    15901 tctttagtag ctggctggtc agggcaaatt tgtcagctgt gtcagcgaag cctggcgcgg
    15961 tcaccatgat cacccacggt gcggcgagca tgccagcgac cgtcacaacc gccagcgcaa
    16021 gtgtcagcag gccagaaaca taagagacaa agacccgcgt ggcgtcttca ccctgcttac
    16081 ttttatattc cgccagaatc ggtacaaatg cctgggaaaa tgccccttcg gcaaagatac
    16141 ggcgtaacaa gttaggaagt ttaaaagcga cgaaaaaggc gtcggttgcc atccctgcgc
    16201 caaagattct ggcgacaatt gcgtctcgtg cgaagccaag cacacgcgaa aacatggtca
    16261 tcgagctgac ggcggccagc gattttaata aattcatcgg tgttctaatc cagacccacg
    16321 ggcaaaacgc ctgcaaggca ggcgctgaaa gaggcgaata gtctacctgg attatggtga
    16381 attgctaccg ccagatgtta cagggtttat tcactcatcg catcgcgcca gatcttgtca
    16441 acgatacgtt gcgccagcac tgcctgttcg ccggcggttt gcggaactgt ctgattttgc
    16501 acacattcaa tgaagtgccg cgcacagccg acaaatccac gttgctcaag cgtactctgc
    16561 caaccaggaa tcggtttatg cactacgccc tgcccgcgct cctcacgcca ttcgcgcata
    16621 tccgtaatgt cgatgagcgc accgtcagtc acggcctgca cggtttcacg ctgacttccg
    16681 gcacggcgat gcatacaggt ggtgatctgc aaaggaccgg ccgaaaaatg gtgctcggca
    16741 aacagcattt cgccagcgtc gttagtcagt agcgtaccgc catccagaga ggctttgccg
    16801 cccgacaacc acagcgcggt atccaccaca tgcagataat catccagcaa cgtgaaataa
    16861 agatcgtgtg gcccgacgct attgctacga tgtttatcca ttcttagcga ggctgcggtg
    16921 gcgagttgcg tttttaactc accgtagagt ggtgcgaaac gacggttaaa accgaccatc
    16981 aacgtcagtt ttttccgcgc cgccagttcc accagccgtt cagcatcgcg cagattttct
    17041 gccagcggtt tatcgacaca gacatgtacc cccgcattga gtaacgtact gaccacgtca
    17101 aagtgactgg cggtgctgga atgtacaaaa accgcatcgc aactggcggc aaggctggat
    17161 aacgaatcgg cataaggaat gcgccagctt tcacaaattg gcagggcttt cgcgcgcgta
    17221 ggcgaccagg ctccttgtaa cgtccagtca gacgctgccg ccagcaccgg taaccacgct
    17281 ttttgagcaa tgccgcctaa tcccactacg ccgatacgta attttttcac tttaatctcc
    17341 caggtgggcc agcaacgaat caagacgctg tttcagttct gccacttcga tttccagggc
    17401 ttcgacgcgg gcctgtaaat caccgtcaac cgcgctcgac atatccgtta ccgacggctg
    17461 atcctctacc tcaccgctga aaagatgcat gtagcggctt tcacgtttac ccggttcgcg
    17521 ggccagacgc accacaaaag gaccatcttc gcgattcgcc agttgttcca gcgtcgactc
    17581 cacttccgcc atatcgctga attcatacat tcgcgcggcg cggctgcgca gttcacccgg
    17641 cgtctgggca ccacgcaata acaacgtggt gattaacgcc acttctgcgg cgctcagttt
    17701 cagatcgcca aattctgaat tacaaaaacg ttgctcatat ttggtgaccc gattaccaaa
    17761 accgctcact gtgcgtagat aatgacgttt gaccagatta tccagctgtt cctgcacttc
    17821 agattcactc agattcatga ccggttcacg gttcgttttc tgattacagg ccgtgactac
    17881 accattgact gagagcgggt attgctccgg cgtggtcacc tgcttttcca gcaagcaacc
    17941 aatcacacga gcttccaggg cagtaagttg atatttcatg agattctcct tagcggccgg
    18001 gcgtccagtc tggggtagtt aatgccgtca gtacgtgatc gcgccattgt ccatcaatca
    18061 acagatagtc tttcgcatag ccttcttttt caaaacccag tcgcgccagt aaatcaccgc
    18121 tgcgtttatt gtgcggcata taattagcca taatgcgatg aatatgttgg gtgcgctgca
    18181 tataacgaat ggctgcggtc agggcttcaa acatgagtcc tttgccctgc catttttgcc
    18241 caatcgaata accgagatag caggcatgaa aagagccgcg aacaacattg gaaaaattgg
    18301 caacgccaat aatctctttt tcgtccgggt cgaataaacc aaagtagaaa gctgaacctt
    18361 gtttatgaaa ttcgttaatc atccccagcc tggcctgcca gcctgatgga tagcagtggc
    18421 tttcgtcgcg cactggctcc cagggcttga ggaaatggcg attctctgcg taataatccg
    18481 ccagacgcca ggcatcacga tcatgcacca gacgcacgac cagtcggtct gtggttaagc
    18541 gcacttttgg cacgttactg cgatagccaa acattctata cctactcctt cccgtaacgt
    18601 ctttaatacg ctgtaagtat tactatacct gcgcaaccag accctgtgaa aagtccaaaa
    18661 tgccattttc tgcattattc acatatttga taaaaactct ctatcaaagc gacgttttga
    18721 taaaaaaata ttgtccccga agtgatggga aaagtcttag cggcaatgca gaatagaaag
    18781 tgctcatctt ttatttcccg ggaggggaaa tgtcccgcgt atcgcaggcg aggaacctgg
    18841 gtaaatattt cctgctcatc gataatatgc tggtcgtgct ggggttcttt gttgtcttcc
    18901 cgctgatctc tatccgcttc gttgatcaaa tgggctgggc cgccgtcatg gtcggtattg
    18961 ctctcggtct acgccagttt attcagcaag gtctgggtat tttcggcggc gcaattgccg
    19021 accgctttgg tgccaaaccg atgattgtta ccggtatgct gatgcgcgcc gccggattcg
    19081 ccacaatggg tatcgcccac gaaccgtggc tattgtggtt ttcatgcctg ctctcgggac
    19141 tcggcggcac gttgtttgat ccgccgcgtt cggcgctggt ggtgaaacta atccgtccac
    19201 aacaacgtgg tcgttttttc tcgctgttga tgatgcagga cagtgccggt gcggtcattg
    19261 gcgcattgtt ggggagctgg ctgttgcaat acgactttcg cctggtctgc gccacagggg
    19321 ccgttttgtt tgtgctgtgt gcggcgttca atgcgtggtt gttaccggca tggaaactct
    19381 ccaccgtacg cacgcccgtt cgcgaaggca tgacccgcgt gatgcgtgac aagcgttttg
    19441 tcacctatgt cctgacgctg gcgggttact acatgctggc tgtacaagtg atgctgatgc
    19501 tgccaattat ggtcaacgac gtggctggcg caccctctgc cgttaaatgg atgtatgcca
    19561 ttgaagcgtg tctgtcgtta acgttgctct accctatcgc ccgctggagt gaaaagcatt
    19621 ttcgtctgga acaccggttg atggctgggc tgttgataat gtcattaagc atgatgccgg
    19681 tgggcatggt cagcggcctg caacaacttt tcaccctgat ttgtctgttt tatatcgggt
    19741 cgatcattgc cgagcctgcg cgtgaaacct taagtgcttc gctggcggac gcaagagctc
    19801 gcggcagcta tatggggttt agccgtctgg gtctggcgat tggcggcgct attggttata
    19861 tcggtggtgg ctggctgttt gacctgggca aatcggcgca ccagccagag cttccgtgga
    19921 tgatgctggg aattattggc atcttcactt tccttgcgct gggttggcag tttagtcaga
    19981 aacgcgccgc gcgtcgtttg ctggaacgcg acgcctgatt tgatactccc ccctgactcc
    20041 tccatactga aagtgaactg acagccaaat ggaggagtca cgtgaagcta tacatttacg
    20101 atcactgccc ttactgcctc aaagcccgca tgattttcgg cctgaaaaat atccccgtcg
    20161 aattacatgt tctgctcaac gacgacgcag aaacacccac ccggatggtc ggtcaaaaac
    20221 aggttcccat tctgcaaaaa gatgacagcc gctatatgcc agaaagcatg gatatcgttc
    20281 actatgtcga taaactcgac ggcaaaccgt tactgaccgg caaacgttcc cctgccattg
    20341 aagagtggct gcgcaaggtc aatggctacg ccaacaaact gctgttgccg cgttttgcca
    20401 aatcggcatt tgatgagttt tctactcccg ccgcgcgcaa atatttcgtc gacaagaaag
    20461 aggccagcgc gggtaatttt gccgacctgc tggcccactc tgacggtctg attaagaata
    20521 tcagcgatga tttgcgtgcg ctggacaaac tgatcgtcaa accgaacgcc gtgaatggcg
    20581 aactttcgga agatgatatt cagctatttc cgctactgcg taatctgacg ctggtagccg
    20641 gaatcaactg gccaagccgc gttgctgatt accgcgataa tatggcgaaa cagacacaaa
    20701 tcaatttgtt atcatcaatg gcgatttaat cctgacccgc cgcggcagcc tgtattcgcg
    20761 ggttgccgcc gttttcagtt ttttctgctg gcgagtttat cgctggtgac acatgctgcg
    20821 ttgtgtcagg atattgacgc agatttcatg tcgaggacta ccatgaacaa gtttttattt
    20881 gccgctgcat tgatcgtcag tggcctgctc gttggctgta atcaactcac ccaatacacc
    20941 atcaccgaac aagaaattaa ccagtcgctt gcgaaacata ataatttctc aaaagatatc
    21001 ggtttacccg gcgtggctga cgcccatatt gttctgacaa acttgaccag ccaaattggt
    21061 cgcgaagagc cgaataaggt taccctaacc ggagacgcca atctggacat gaactccctg
    21121 ttcggtagtc agaaagcgac catgaaactg aagctgaaag cgctgcccgt gtttgataaa
    21181 gaaaaaggtg cgatcttcct gaaagagatg gaagtggtcg atgcgacggt acaaccggaa
    21241 aaaatgcaaa cggtgatgca aacgttgctt ccctatttga accaggcatt acgcaattac
    21301 tttaaccagc aacctgctta cgtcctgcgc gaagatggcg gtcagggcga agcaatggcg
    21361 aaaaaactgg cgaaaggcat tgaagtgaag ccaggcgaaa ttgtcattcc gtttacggat
    21421 taatgacgag ggcgcattcg cgccctttat ttttcgtgca aaggaaaacg tttccgctta
    21481 tcctttgtgt ccggcaaaaa catcccttca gccggagcat agagattaat gactgcacca
    21541 tcccaggtat taaagatccg ccgcccagac gactggcacc ttcacctccg cgatggcgac
    21601 atgttaaaaa ctgtcgtgcc gtataccagc gaaatttatg gacgggctat cgtaatgccc
    21661 aatctggctc cgcccgtgac cacagttgag gctgccgtgg cgtatcgcca acgtattctt
    21721 gatgccgtac ctgccgggca caatttcacc ccattgatga cctgttattt aacagattcg
    21781 ctggatccta atgagctgga gcgcggattt aacgaaggcg tgttcaccgc tgcaaaactt
    21841 tatccggcaa acgcaaccac taactccagc cacggcgtga cgtcaattga cgcaatcatg
    21901 ccggtacttg agcgcatgga aaaaatcggt atgccgctac tggtgcatgg tgaagtgaca
    21961 catgcagata tcgacatttt tgatcgtgaa gcgcgcttta tagaaagcgt gatggaacct
    22021 ctgcgccagc gcctgactgc gctgaaagtc gtttttgagc acatcaccac caaagatgct
    22081 gccgactatg tccgtgacgg aaatgaacgg ctggctgcca ccatcactcc gcagcatctg
    22141 atgtttaacc gcaaccatat gctggttggt ggcgtgcgtc cgcacctgta ttgtttaccc
    22201 atcctcaaac gcaatattca ccaacaggca ttgcgtgaac tggtcgccag cggttttaat
    22261 cgagtattcc tcggtacgga ttctgcgcca catgcacgtc atcgcaaaga gagcagttgc
    22321 ggctgcgcgg gctgcttcaa cgccccaacc gcgctgggca gttacgctac cgtctttgaa
    22381 gagatgaatg ctctgcagca ctttgaagca ttctgttctg taaacggccc gcagttctat
    22441 ggcttgccgg tcaacgacac attcatcgaa ctggtacgtg aagagcaaca ggttgctgaa
    22501 agcatcgcac tgactgatga caccctggtg ccattcctcg ccggggaaac ggtacgctgg
    22561 tccgttaaac aataaaaatt gtagcttcct gttgtcatta ggttatttta cctgtataaa
    22621 taaccagtat attcaacagg gggctattat gcgaattgaa gtcaccatag cgaaaacttc
    22681 tccattgcca gctggggcta ttgacgccct ggctggcgaa ctttcccgcc gtattcagta
    22741 tgcgtttcct gataatgaag gccacgtatc ggtacgttat gccgcagcga ataatttatc
    22801 ggttattggc gcaacaaaag aagataaaca gcgcattagc gaaattctcc aggaaacgtg
    22861 ggaaagcgcc gatgactggt ttgtcagcga ataatatgca gtgatttttg tttttgccgg
    22921 gtcgccccgg cttttttatt ttacttacaa attttgagta caaaatttct acacttcctc
    22981 aaaataatt
//
LOCUS       DAJOWB010000072        22866 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJOWB010000072 DAJOWB010000000
VERSION     DAJOWB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.072"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..1034
                     /locus_tag="OHO85_002991"
                     /pseudo
     CDS             1..1034
                     /locus_tag="OHO85_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1291..1710
                     /locus_tag="OHO85_002992"
     CDS             1291..1710
                     /locus_tag="OHO85_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCQ2672338.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMVELASQPGACVVQIARENGVND
                     NVIFKWLRLWQNEGCISQRLPVTTTSDAGVELLPVEITPDEPKEPVAALTPSLSTQTT
                     VSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            1707..2057
                     /gene="tnpB"
                     /locus_tag="OHO85_002993"
     CDS             1707..2057
                     /gene="tnpB"
                     /locus_tag="OHO85_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCQ2672339.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKISITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            2088..2828
                     /locus_tag="OHO85_002994"
                     /pseudo
     CDS             2088..2828
                     /locus_tag="OHO85_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3057..4289)
                     /locus_tag="OHO85_002995"
     CDS             complement(3057..4289)
                     /locus_tag="OHO85_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2672340.1"
                     /translation="MNKWMKLCFLILGGGTIFKLSSMKDVFYVPMQADWGLTNTQIGF
                     GFTIYAAVQTIGLFSSLYIADRFSKKILLPAGLIGVGLCGAYLTTLPPFSGYLIAFGA
                     MAFFGEVVYWPVLLKAVRLLGNRDEQGRMFGFLEAGRGVVDVLIASGALFVFVQFGEG
                     KAGMQAGLVYYTLITIIVGIITYFIVDADDRTIPQNDEDVNKQIFKGIKNVVKSPNLW
                     LASFCIFFVYSAYCGLTYFIPFLKDIYALPVALVGAYGIINQYGLKMVGGPVGGYLAD
                     KISHSPVIYLKWTFLISAVAMLLFIQLPHDSMNVYLGMTATLGFGAIIFSQRAIFFAP
                     MDEIGTPREFAGSAMAFGCIIGYMPSMFAYTLYGSLLDNFSGIQGYNYVFSVMVAFSL
                     LGFICATLLTRRMKMRNE"
     gene            complement(4304..4681)
                     /locus_tag="OHO85_002996"
     CDS             complement(4304..4681)
                     /locus_tag="OHO85_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCQ2672341.1"
                     /translation="MIIDTKHAPKAIGPYVQAVSTGNLLFISGCCPFKPEDGTVNGET
                     IERQTRQAMDNLKAIIESAGIGMDNIVKTTCFISNMDNFSQFNLLYSEYFSSDKYPAR
                     SCVEVARLPKDVLIEIEAIAVIK"
     gene            complement(4703..5662)
                     /locus_tag="OHO85_002997"
     CDS             complement(4703..5662)
                     /locus_tag="OHO85_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCQ2672342.1"
                     /translation="MKLGLETESYHLFFQQGMMDIFDFIDKTSELGLDGVEINIIPDV
                     GLHPEFGVLNSISPEYLSKVRKSIDSHGLYCEIDTRFTSCEAITRAVEIASALGAEVI
                     RTYMFRRGEYNPLKYPEIVEQLKSLTSLLSRKKIKLAIENHEDETADEIIEVIKSVNS
                     EWVGAHCDIGNGMMAWEEPVETVRKLAPYAYSTHFKDHIVTRNGEDLVVCGVPVGEGS
                     IDIDTCFKTLVKESSLTRINIETCFPYASPFARPKGTGGVYSLEGAFSVKEAPFEHLK
                     IKPLDYYYPAKISDSVLQELMAAQNRCVEVSVQTLKNLRKKYC"
     gene            5848..6510
                     /locus_tag="OHO85_002998"
     CDS             5848..6510
                     /locus_tag="OHO85_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HCQ2672343.1"
                     /translation="MKSIVNHEFTEDDRQLLLSYIPFIDSLGQFLGEYCEIVLHSFED
                     LHHSVIHIVNGHVTGREKGAPVTNIALEKLAEFNSTNDMWDVYFNHKGVRPFKSSSSL
                     ILNKNKEPVGMICMNFALDMPLNAFIENFAKSSNCKNENFTQDINNLVLSHLEPVKNM
                     VYSNKNIPSKNKNFEIVKELNSIGLFKFPVTTKIVSSVLGISANTIYKHLRVLNNKGK
                     EN"
     gene            complement(6628..6774)
                     /locus_tag="OHO85_002999"
     CDS             complement(6628..6774)
                     /locus_tag="OHO85_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001486734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCQ2672344.1"
                     /translation="MFSKSATELRPKLPALLEDAAMLYLRIGTCRLNNMAPEKWLRYV
                     IEHI"
     gene            7443..8363
                     /locus_tag="OHO85_003000"
     CDS             7443..8363
                     /locus_tag="OHO85_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCQ2672345.1"
                     /translation="MSTRVWVLGDAVVDLLPESQGRLLQCPGGAPANVAVGIARLGGK
                     SAFIGKVGDDPFGRFMYQTLSTENVDTRYMSLDPQHRTSIVAVGLDEQGERNFTFMVR
                     PSADLFLQPDGLPAFGPGEWLHLCSIALSAEPSRSTAFLAMEKIRQAGGNISFDPNIR
                     SDLWQSEALLRKYLDRALSLANIAKLSEEELLFISGESQVQQGAYSLVQRYSLTLLLI
                     TQGKNGVLVYFQGQFIHYPAKPVSVVDTTGAGDAFVAGLLAGLADSGIPTNTRQLERI
                     IVQAQICGALATTAKGAMTALPRQHDLPSQ"
     gene            8531..10048
                     /locus_tag="OHO85_003001"
     CDS             8531..10048
                     /locus_tag="OHO85_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HCQ2672346.1"
                     /translation="MYKKTTLAVLIALLTGATTVHAQTDISSIESRLAALEQRLKNAE
                     SRAQAAEARAKTAELQVQKLAETQQQNQLTTQEVAQRTVQLEQKSAENSGFEFHGYAR
                     SGLLMNDAASSSKSGPYLTPAGETGGAVGRLGNEADTYVELNVEHKQTLDNGATTRFK
                     AMLADGQRDYNDWTGGSSNLNIRQAFAELGALPSFTGAFKDSTVWAGKRFDRDNFDIH
                     WLDSDVVFLAGTGGGIYDVKWNDTFRSNFSLYGRNFGDLDDIDNNVQNYILTMNHYAG
                     PFQLMVSGLRAKDNDDRKDANGDLIQTDAANTGVHALVGLHNDTFYGLREGTAKTALL
                     YGHGLGAEVKGIGSDGALLSEANTWRFASYGTTPLGSGWYVAPAILAQSSKDRYVKGD
                     SYEWVTFNTRLIKEVTQNFALAFEGSYQYMDLKPKGYQNHNAVNGSFYKLTFAPTLKA
                     NDINNFFSRPELRLFATWMDWSSKLDDFASNDAFGSSGFNTGGEWNFGVQMETWF"
     gene            10109..11479
                     /locus_tag="OHO85_003002"
     CDS             10109..11479
                     /locus_tag="OHO85_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HCQ2672347.1"
                     /translation="MDFDKIAQSLLPLLGGKENIASAAHCATRLRLVLVDDTLADQHA
                     IGQIDGVKGCFRNSGQMQIIFGTGVVNKVYAAFIQVAGISESSKADIARLAAQKLNPF
                     QRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVNTDNAIYILLDMCSSAAFIIL
                     PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFQTMNFFGFEIAMIGYQG
                     TVFPVLLAVWFMSIVEKQLRRFIPDALDLILTPFLTVVISGFIALLIIGPAGRALGDG
                     ISFVLSTLIAHAGWLAGLLFGGLYSAIVITGIHHSFHAIEAGLLGNPAIGVNFLLPIW
                     AMANVAQGGACLAVWFKTKDTKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAAL
                     IGGAAGGAWVVSVHVYMTAVGLTAIPGMAIVQPTSLVNYIIGMVIAFAVAFSLSLLFK
                     YKTDEE"
     gene            11479..12888
                     /locus_tag="OHO85_003003"
     CDS             11479..12888
                     /locus_tag="OHO85_003003"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCQ2672348.1"
                     /translation="MSMPSQMPAILQAVMKGQSKALADAHYPCWHLAPVTGLMNDPNG
                     FCWSGGRYHLFYQWNPLACDHKYKCWGHWSSTDLLHWQHEPLALMPDKEYDRNGCYSG
                     SAVNNQGVLTLCYTGNVKFDDGSRTAWQCLATENNQGGFDKLGPVISLPDGYTGHVRD
                     PKVWKHNSQWYMVLGAQDKEKRGKVLLYSSVDLNTWSFHGEIAGNGLNEIDNAGYMWE
                     CPDLFALDGEYILLCCPQGMVREHERYLNTYPCAWLHGQFDYETGKFTHGAFSELDAG
                     FEFYAPQTMEAPDGRRLLVGWMGVPDGEEMLQPTRKHHWQHQMTCFRELSFQKGKLFQ
                     MPIRELAQLREAEHFWQGKADHAPHVEIERLEMDIIPSGELYLNFGNALALHLNDDGI
                     QLQRRSLAGQEKLTRYWRGSVTSLKILCDSSSVEIFINNGEGVMSNRYFPHHPASLIL
                     QGESDVTLRYWSLRACMVE"
     gene            12914..13921
                     /locus_tag="OHO85_003004"
     CDS             12914..13921
                     /locus_tag="OHO85_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCQ2672349.1"
                     /translation="MRKTKRVTIKDIAELAGVSKATASLVLNGRGKELRVAQETRERV
                     LSIAQQQHYQPSIHARSLRDNRSHTIGLVVPEITNYGFAVFSHELETLCREAGVQLLI
                     SCTDENPSQETMVVNNMIARQVDGLIVASSMLHDNDYQKLSEQLPVVLFDRHMNGSTL
                     PLVITDSVSPTAALVADIARSHPDEFYFLGGQPRLSPTRDRLEGFTQGLQQAGVTLQP
                     EWIIHGNYHPSSGYEMFAALCARLGRPPKALFTAACGLLEGVLRYMSQYNLLDSTIHL
                     ASFDDHYLYDSLSVRIDTIQQDNRQLAFHCFELISQLIEGETPSPLQRYLPASLQKRY
                     R"
     gene            complement(14473..14946)
                     /locus_tag="OHO85_003005"
     CDS             complement(14473..14946)
                     /locus_tag="OHO85_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HCQ2672350.1"
                     /translation="MCKLTKEEEYSIISRTMTNIRSLRTYVRELDFEQLLDMQEKLNT
                     VIEERREDAERKAAERKELETKCQQVFEYIVSVGLDPEEWQGPVSAIAGTTETKRKPK
                     GGVRKAKYIFEDENGETRTWSGNGKMPLALRKQVNGDCTLETFLIEKPNQHEQSE"
     gene            15265..16002
                     /locus_tag="OHO85_003006"
     CDS             15265..16002
                     /locus_tag="OHO85_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HCQ2672351.1"
                     /translation="MKKVIAVSALAMAGMFSAQTLADESKTGFYVTGKAGASVMSLSE
                     QRFVDGEGAWADKYKGGDKSDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGNAESKY
                     RLSYWESVGGAEFDDAQNKLSVNTLMLNAYYDFRNSSAFTPWISAGLGYARVHHKTSY
                     IYTDNSPAGSEVYSASASKYENNLAWSLGVGVKYDVTQDFSLDLSYRYLDAGDSTLTY
                     KDEDGAKYKSSVDVRSNEFMLGATYNF"
     gene            16422..17318
                     /locus_tag="OHO85_003007"
     CDS             16422..17318
                     /locus_tag="OHO85_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HCQ2672352.1"
                     /translation="MADSTRNRSAERLIDILVELQNYGVVSRHNLMKKYNITERTAYR
                     DLNMLSPFIEACGDGKYRLISARAGNQSKGSLHKSLARLLDTDAIFPERDEGFWQKLE
                     NRATEKHIRVQFHNPEHTIRDDLRKYLDVLEKAIRNSNICQIAYAGKTRIVHPYKLTN
                     QRSIWYLLATEENKLKSFSLAKIKWLDIKKEKFAKSEEIQSLISESCDPWVSDKTFDV
                     VLFIHSNIAHYFLRRDLLPYQQLLHRHDNGITLSCKAAHKNQIIPLILYWLPNVEIIE
                     PVWLKEAVLTMLGKYLTAENVS"
     gene            17367..18446
                     /locus_tag="OHO85_003008"
     CDS             17367..18446
                     /locus_tag="OHO85_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HCQ2672353.1"
                     /translation="MALPFIILGVAAGAAAFGGKKAYDGYQKKSEAEDVLEKAKERYE
                     EHKGKFEQAEAQTSSKLTTLGNKELEIGKDFSEFDRIAAELLARLEKEGNKDLKLSVP
                     QHNINKIHNLAISATEYLSTVVAAGVSGAAAGFAVYSGVMAFAAASTGTPIAALSGAA
                     AYNATMAAIGGGSLAAGGMGMAGGAMVLGTAVAAPLIAIAGWAYDRHATKALENAHQC
                     ACKVERAVKKMNLAREHFAKVNQYVDEILAALNRMHAVFIKHYFEPLKSMHSVITEQQ
                     DINVSDEIIMLIDHGYSLAAIMTDIITTPLFKPRKQENGEAVIKDNVIEMEKDDNGMN
                     VINKEELDDVLASSVVKFDDFSSRS"
     gene            18493..20064
                     /locus_tag="OHO85_003009"
     CDS             18493..20064
                     /locus_tag="OHO85_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--cob(I)alamin adenosyltransferase"
                     /protein_id="HCQ2672354.1"
                     /translation="MNVEKFDWAEELHQTMVKSLVASFGLDFLLLNDRKGGDVDTIHN
                     VRNGIYATEAERQRYEGRGDYDSHHYHSHENYIATNRKGKRAHQSGTLTDAYTGEVFA
                     SDDKKNLDHIISAKEIHDDPGRILAERDGAELANDASNLAFTHESLNKSKKADSMDAF
                     ICTLQKNREDTLRQIAELESQATLTEKEKKRLISLRKKADADTERMERADKYAREKYE
                     TTINQSYYLSSKFATAVASASLNSGFRMGTRQMLGLILAEIWFELRERIPHIIRQQRN
                     SFCAGKCLSEITSALRACQERVQEKFSSFLIAFKDGVIGGILSGITTTLLNIFLTTER
                     MIVRLIREMWNNLVQAFKVLVFNPEGLSPGQLAKAVTKLIAAGVAVAGGVMINEAMAQ
                     ILIFPFGSELAAFCGALTTGILGLVMNYYLEYSEVMQRVWAFLDKFKDKFQRSLEYFQ
                     AVNAELDRYLVELTALEFSVDAAALNHFSLHLDSVNSEIERGLLLREEVSRRGIALPF
                     ESGNVASVRDWLNKR"
     gene            20467..20664
                     /locus_tag="OHO85_003010"
     CDS             20467..20664
                     /locus_tag="OHO85_003010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672355.1"
                     /translation="MRLLLKGLVGMALVWLVIGFGMELFFLADRVHPALRVIVGLGML
                     FLMIMIAGGMCRSLLSRKKTS"
     gene            20717..22390
                     /locus_tag="OHO85_003011"
     CDS             20717..22390
                     /locus_tag="OHO85_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCQ2672356.1"
                     /translation="MNSPVTACSATSAGQKYRFYTIIMKINLPFIHWPRICNDKCLVL
                     SVGVFTLSNLLVSSGAHLIYRVLFFILAILIIKAMKSLTLRFILAFPFTLLTAADISI
                     SLYSWCTFDTTFNDGFAISILQTDPDEAIRMFRMYVVYVIAFIILFLLFFCSAINKTS
                     SLPSEKVTVITFLLLITVTLYSSFQFALKKQYQINEVDPYIVASRFATYTPFFNLNYF
                     ALAAKEHQRLMTIADTIPHYDLVITDNNTDVFVLVIGESARTDNMSIYGYSRPTTPEL
                     QKQKSRLKLFTQAISGAPYTALAVPLALSADSVLHHDIRNYPDNIINMANQAGFDTWW
                     LSAQSAFRQNGTAVASIAMRARNRIYVRGYDELLLPHLAEALNSNPGSRKLIVLHLTG
                     SHEPVCSNWPRDKAVFKPLDTEEVCYDNSIHYTDSLLGQVFTMLETRQASVMYFSDHG
                     LEYDPTKEHAYFHGGIKPSQQAYHVPMFIWYSPTLGEHVDRQTVNSVFSTAYNDYLIN
                     AWMGVTRPAQPKTPEEVITRWQGKSVVFDASHNVFDYKVLRKNFNELQE"
     gene            complement(22534..>22866)
                     /locus_tag="OHO85_003012"
     CDS             complement(22534..>22866)
                     /locus_tag="OHO85_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCQ2672357.1"
                     /translation="DTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERAEIIKCA
                     EMLMQEDDFEICESILRQQKLLRERIDETLSEEIVQKCRNINGEYVWPWILPFSAAGM
                     KHSSIQHQ"
BASE COUNT         6223 a         4906 c         5421 g         6316 t
ORIGIN      
        1 caggtcgttc ctgcctctga acttgctgct gccatgaagc agattaaaga actccagcgc
       61 ctgctcggca aaaaaacgat ggaaaatgaa ctccttaaag aagccgttga atacgggcgt
      121 gcaaaaaagt ggatagcgca cgcgccctta ttgcccgggg atggggagta agcttagtca
      181 gccgttgtct ccgggtgtcg cgtgcgcagt tgcacgtcat tctcagacga accgatgact
      241 ggaaagatgg tcgccgcagc cgtcactcag atgatacgga tgtgcttctc cgtatacacc
      301 atgttatcgg agagctgccc acatatggtt atcgtcggat atgggcgctg cttcgcagac
      361 aggccgaact tgatggtatg cctgcgatca atgccaaacg tatttaccgg atcatgcgcc
      421 agaatgcgct gttgcttgag cgaaaaaccg ctgtaccgcc atcgaaacgg gcacatactg
      481 gcaaagtggc cgtgaaagaa agcaatcaac gatggtgctc tgacgggttc gagttccgct
      541 gtgataacgg agaaaaactg cgagtcacgt tcgcgctgga ctgctgtgat cgtgaggcac
      601 tgcactgggc ggtcactacg ggcggcttcg acagtgaaac agtacaggac gtaatgctgg
      661 gagcggtgga acgccgcttc ggcaacgagc ttccggcgtc tccagtagag tggctgacgg
      721 ataatggttc atgctaccgg gctaatgaaa cacggcagtt tgcccggatg ttggggcttg
      781 aaccgaagaa cacggcggtg cggagtccgg agagtaacgg catagcagag agcttcgtga
      841 aaacgataaa gcgtgattac atcagtatca tgcccaaacc agacgggtta acggcagcaa
      901 agaaccttgc agaggcgttc gagcattata acgaatggca tccgcatagt gcactgggtt
      961 atcgctcgcc acgggaatat ctacggcagc gggccagtaa tgggttaagt gatgacaggt
     1021 gtctggaaat ataggggcaa atccatttcg caagcaaaga ctctggtaaa tactcgtcat
     1081 atttatccag atccttatca ggaacaactg tagcaggctc aaattctgaa ggtgcaaaat
     1141 ggcatctgaa tcgatcacga cttggcatgc ttccgatcat tgattggcat cctgccagtc
     1201 gtatcgtaag cgtaaaccga ccgccgtatg tagccattag acaagaattg gtaacttaga
     1261 cgcccatctg acacagacgg acatctaagt atggaattgc aggactggcg aaaagaacct
     1321 cgtaaaaagt attcgaatga attcaaactt cgtatggttg aactggcatc acaacccggt
     1381 gcttgtgtgg tccagattgc acgagaaaat ggcgtcaatg ataatgttat attcaaatgg
     1441 ctcagactct ggcaaaacga agggtgtata tcgcagcgtc ttccggtgac aaccacttct
     1501 gacgcaggcg ttgaattatt acctgtagag ataacgcctg atgagccgaa agaaccggtg
     1561 gctgctctta ctccgtcttt atccacccag actactgtca gtgccagctc ctgcaaggtg
     1621 gagttccgtc acggtaacat gacgctggaa aatccttcac cagagctgct cactgtgttg
     1681 atccgtgaac tgaccgggag gggacgatga tctcactccc gtcaggcacc cgcatctggc
     1741 tcgttgccgg cgttaccgat atgcgtaaat ccttcaacgg actgggagaa caggtacagc
     1801 atgtgctgaa tgataatccc ttctccggtc acctgttcat cttccgtggc cgacggggtg
     1861 acacgattaa aatcctgtgg gctgatgctg atggtctgtg cctgttcacc aaacgcctgg
     1921 aggaaggcca gtttatctgg cctgcggtgc gtgacggcaa gatatccatt acccgctcgc
     1981 aactggcaat gctcctcgat aagctggact ggcgtcagcc aaaaacatcc cgtcttaacg
     2041 cactgacaat gttgtaaaaa acgcctggcc gcattataaa aacggccatg agtcagaaat
     2101 acctcattcg cattgcagaa ctggaaagcc agctccgtca gaaagaccag caactgagtc
     2161 tggttgaaga gacggaggcc ttcctgcgtt ctgcgctggc acgtgccgaa gaaaagatcg
     2221 aagaagatga gcgggaaata gagcatctgc gggcccaggt ggaaaaactg cgccggatgc
     2281 tgttcggtac ccgttctgaa aaactgcgtc gtgaagttga acaggctgaa gccctgctga
     2341 aacgacgcga acagaacagc gatcgttaca gcgggcgtga ggatgacccg caggttcccc
     2401 gccagttgcg acagtcgcgt catcgtcgcc cgttaccggc gcatctgccc cgcgaaataa
     2461 accgcctgga gccagaagaa agctgttgcc cggagtgtgg tggtgagctg gattatctgg
     2521 gggaagtcag cgcagaacaa ctggaactgg tgagcagcgc cctgaaagtg atccgcacag
     2581 aacgggtaaa aaaagcctgt acaaaatgtg actgcatcgt tgaagcaccg gcaccgtccc
     2641 gtccgatagc gcgtggtgtc gcgggcccgg ggttacttgc ccgcgtgtta acgggaaaat
     2701 actgcgaaca cctgccactg tatcgtcaga gtgaaatctt tgcccgtcag ggtgtcgaac
     2761 tgagccgtgc gttactctcc aactgggttg acgagctcct gccatggaac gtagcactca
     2821 ccaataaata agtatcaata cggcactccg ttaacgctta ctgaaacgac gccggggacc
     2881 atcttctttt gggtaaaaaa ggccgcgcca gaattcgaca cgaccactgc tatatgcgta
     2941 ttattctgtt ccgggagatt gcctctcttc tggttattca gcaatacaac ggacaaaaag
     3001 cactggcata aataacccct tgttaaacgg agaatagtga taatgtattg acgctattac
     3061 tcatttctca tcttcatcct tcgggtaagc agagtcgcac aaatgaatcc cagcagacta
     3121 aatgccacca ttacagaaaa aacatagtta tagccctgta tgccagaaaa attatctaac
     3181 agcgaaccat aaagcgtgta agcaaacatg gaaggcatgt aaccaataat gcacccaaac
     3241 gccattgctg aaccagcaaa ttctctgggg gtaccgattt catccattgg cgcaaaaaaa
     3301 attgcccgtt gtgaaaaaat gattgcacca aatccaagag ttgcagtcat tccaagataa
     3361 acattcattg agtcgtgtgg tagttgaata aaaagtagca tagcgactgc tgaaataagg
     3421 aaagtccatt tcagatagat gaccggagaa tgagaaattt tatctgcgag ataaccgcca
     3481 actggcccgc ccaccatttt tagtccgtac tggttaataa tcccgtaagc acccacgagt
     3541 gcaacgggaa gggcatagat atctttcagg aatggtatga aatatgttag cccacaatat
     3601 gcggagtaca caaagaatat acaaaatgag gccagccaca gatttggtga tttaacgaca
     3661 ttctttatcc ccttgaatat ctgtttatta acatcttcgt cattctgtgg aatggtcctg
     3721 tcgtctgcat ccacaataaa gtaggtgata attccaacaa taatggtgat taatgtgtaa
     3781 taaacaagac cagcctgcat gccagctttt ccttcgccaa actgcacaaa gacgaataaa
     3841 gcgccggacg caattaatac atccacgact cctcgtccag cttccagaaa gccgaacata
     3901 cgtccctgtt catccctgtt ccccaacaga cgaacagctt taagtagcac gggccagtag
     3961 accacttcgc caaagaaagc catagcacca aaagctataa ggtaaccaga gaaggggggt
     4021 aatgttgtga ggtaagcgcc gcacagaccc acaccgatta aacctgcagg aagaagtatt
     4081 tttttggaaa agcgatctgc tatatataat gaactaaata gtccgatagt ctgaacagca
     4141 gcatatattg taaatccaaa gccaatctgt gtattagtca atccccagtc agcctgcata
     4201 ggaacataaa atacgtcctt catactggac aatttgaaaa ttgtcccacc gccaagaata
     4261 aggaagcata gcttcatcca tttattcatc tggttatccc ccattatttg ataaccgcaa
     4321 tggcttcaat ttctatcagc acgtctttcg gtaatctggc gacttcaaca caggacctgg
     4381 ccgggtattt gtctgatgag aaatattcac tgtacagaag gttaaattga gaaaaattat
     4441 ccatgttact gataaaacag gttgttttta cgatgttatc cattccgatt cctgcagatt
     4501 caataattgc cttcagatta tccatcgctt gcctggtttg cctttctata gtctcgccat
     4561 ttacggttcc gtcttctggt ttgaaaggac aacatccgga aatgaacaat aaattaccag
     4621 ttgatactgc ctgtacatag ggaccgatag ctttgggggc atgtttagta tcaatgatca
     4681 taaaagtatc cttttaaatt tattaacaat atttttttct taaattcttc agagtctgaa
     4741 cagaaacctc aacacaacga ttttgggctg ccattagttc ctgtaataca gagtcagata
     4801 ttttggcagg gtagtaataa tcaagtggct ttattttcag gtgttcaaat ggtgcctctt
     4861 ttacggagaa tgccccttcc agagaatata cgccgccagt accctttgga cgggcaaaag
     4921 gtgaggcata tgggaaacat gtttcgatgt ttattctggt taaagaggac tctttaacca
     4981 gagttttaaa gcaggtatca atatcaatgc ttccttctcc gacaggaaca ccacagacaa
     5041 ccaggtcttc gccgttgcgt gtgacaatat gatccttgaa atgcgtgctg taagcataag
     5101 gagccagttt acgaactgtt tctactggtt cttcccatgc catcattcca ttacctatat
     5161 cacagtgtgc tcccacccac tcagaattaa cagatttgat aacttcgatt atctcgtcgg
     5221 cagtttcatc ttcatggttt tctattgcca gtttaatttt ctttctactg agtaaggatg
     5281 tcagtgattt taactgttct actatttccg gatatttcag agggttatat tctcctctcc
     5341 tgaacatata agttcggata acttctgctc caagtgcaga tgcaatttcc actgctctgg
     5401 tgatagcttc acaggaagtg aatcgggtat ctatctcaca ataaagacca tgagaatcaa
     5461 tggattttct gacttttgac agatattcag gagatatact gttcaggaca ccaaattccg
     5521 gatgcagacc aacatccgga atgatattaa tttcgacccc atccagcccc agttcactgg
     5581 ttttatcaat gaagtcgaaa atatccatca ttccctgctg aaagaacaga tgatatgact
     5641 cagtctcaag gcctagtttc atggcgaacc tccatagtat ttgagactga ttgtagtttt
     5701 ttatacattt gtaaaatgtg atcaaagtag aataatttct cacttagtga ataaatttac
     5761 tacttggtgt tattttgagc cactttggtg gactctgttc tgcatctggt atagcattca
     5821 gatgtacttt gttcatgagg ttattatatg aaatctattg ttaatcatga gtttactgag
     5881 gatgacaggc agcttttgct ctcatatatt ccattcatcg acagtcttgg tcagttcttg
     5941 ggtgagtatt gcgagatcgt cctgcatagt tttgaggact tgcatcattc cgttattcat
     6001 atcgttaacg ggcatgtgac ggggagagag aagggggcac ctgtaacaaa tatagcactg
     6061 gaaaaactgg cagaatttaa ctcaacaaac gatatgtggg atgtctattt caaccataaa
     6121 ggcgttcgcc cttttaaatc atcgtcatca cttattctga ataaaaataa agaaccagta
     6181 ggtatgatat gtatgaactt tgctctggat atgcctctta atgcatttat tgaaaacttt
     6241 gcaaaatcgt caaactgtaa aaacgaaaat ttcactcagg atatcaataa tcttgtgctt
     6301 agtcaccttg aacctgttaa aaatatggtt tactccaata agaatatacc aagtaaaaac
     6361 aagaactttg aaatagtaaa agaacttaat tctattgggt tgtttaagtt cccggttaca
     6421 acaaaaattg tttcatcggt tcttggtatc agtgctaaca ctatttataa acatctcagg
     6481 gtattaaata ataaggggaa ggagaattaa ggtgtctgta ttctgaactt ataagtaagt
     6541 tgtttgtctg aagcgtattg atatttattt ttcggttaga gcaactttct agggaaacag
     6601 atcgcgtatc cagtttgcct gtcattccta gatatgttca atgacgtaac gcagccattt
     6661 ttctggagcc atattgttca gacggcatgt accaatccgc aggtacagca tcgccgcatc
     6721 ctcaagtaat gccggtaatt tggggcgtaa ttctgttgcg cttttactga acacaatgcc
     6781 gtattcagta agaagtccaa gaacatgatc tatcagttgt gtccttccat cgagatcaac
     6841 cgatgcattt tcaaaaaagc ctggacgttc tgtaggtcca gactttttat tggtaccagt
     6901 cgtatagttc tatattgata ggtttcagct attgctgtgc gttgttataa tcatttttct
     6961 gacgccattg ataagattta acatgttgtg ctggatgcaa tattacatca tagttgagtt
     7021 cccttatttc ctgtgggcag tgatgggatg tcccgcaagc cttcatcgca acggtcattt
     7081 tggctgttat accagccagt taagcagttg gttgtgagaa agtttgatct ttcccgcgtg
     7141 attgccacga gcattgatac tgaaaagata aaagttcgtt ttagcctgag cgatgtcggt
     7201 aattttctgt tcaatcatgg cctttctctt acttttgtgg ttaaaattta ttcgctatgg
     7261 cgcaattgaa agcggtcgtg aaggtggggg aagactatct catcacttac catatttccg
     7321 acatattctc acaacaaaat accacgtaac tattgatgaa tattgttctc tcctgagcag
     7381 cgtttatgct ttaattgcta aatcgattta attagtttag caattagtat attggagcct
     7441 ttatgagcac aagagtatgg gtacttggtg atgcggttgt tgatttatta cccgaaagcc
     7501 aggggagact actacagtgt cctggcgggg cgcctgctaa tgttgcagtc ggtatcgcaa
     7561 ggctgggggg gaaaagtgcc tttattggca aagttggcga tgatcctttc ggtcgcttta
     7621 tgtatcagac actgagtaca gaaaatgttg atacacgtta tatgtctctt gatcctcaac
     7681 atcgcacctc aattgtggct gtaggacttg atgagcaagg agaaagaaac tttaccttta
     7741 tggtacgccc aagtgccgat ctttttttac aacctgatgg ccttcctgca tttgggccgg
     7801 gtgaatggct ccatctttgt tccattgcgc tcagtgcaga accttcccga agtaccgcat
     7861 ttctggctat ggagaaaata cgtcaggctg gcggaaacat cagttttgat cccaatatcc
     7921 gcagcgatct ctggcagagt gaagcgctat taaggaaata ccttgatcgc gcactttcgc
     7981 tggcgaatat cgctaaattg tccgaagaag agttgctatt catcagtggc gaaagccagg
     8041 ttcagcaagg cgcatattca ttagtacaac gttattcgtt gactttattg cttatcacac
     8101 aaggaaaaaa tggcgtactt gtgtattttc aggggcagtt tatccactat cccgccaaac
     8161 ctgtttctgt cgtcgatacg accggggcag gagatgcttt tgtcgctgga ttacttgccg
     8221 gtctggctga ttctggaata ccaacaaata ccagacagct tgaacgaatc attgtacaag
     8281 ctcagatttg tggtgctctg gcgaccacgg ctaaaggcgc gatgaccgcc ttaccccgac
     8341 aacacgatct cccttcacaa tagtgatcct gctgttgtat ctgtaacccg atttgtcgtg
     8401 cctgtcacat ttactaaatc ggtttagcga aaataattgc tttggctaaa aagtcagctt
     8461 tattatcaac acctaaaccg gtttagcatt ttagcaattt ttataattct ctgatgggac
     8521 gcgacaaatt atgtataaaa aaacaacttt ggcagtgtta attgctttgc tgaccggtgc
     8581 tacaacggta catgcgcaaa cggatattag cagtatcgaa tctcgactgg cggcgttgga
     8641 acaacgttta aaaaatgcgg aatcccgcgc ccaggcggca gaagcaaggg ccaaaacagc
     8701 tgaattacag gttcagaaac tggctgaaac acaacaacaa aatcagctaa caactcaaga
     8761 agtagcacag agaacagttc agctcgaaca gaaatccgca gaaaacagtg gttttgagtt
     8821 tcatggctat gcccgttccg ggttactgat gaatgatgcc gcttccagta gcaaaagtgg
     8881 gccgtatctg actcccgcag gtgaaactgg tggagctgtt ggccgtctgg gaaatgaagc
     8941 cgatacctat gtcgagttaa atgtagaaca taaacaaaca ctggataacg gtgcgaccac
     9001 acgctttaaa gcaatgttgg ctgacggaca aagagattac aacgactgga ctggcggctc
     9061 cagtaacctg aatatccgac aggcttttgc cgaactgggc gcattaccaa gttttaccgg
     9121 agcattcaaa gacagtactg tctgggctgg taaacgcttt gatcgcgaca attttgatat
     9181 tcactggtta gactccgatg tcgtattttt agcgggaacg ggcggcggta tctatgacgt
     9241 aaaatggaac gatacatttc gcagtaactt ttctctctac ggacgtaatt tcggcgatct
     9301 tgatgatatc gacaataacg ttcagaatta catcctcacc atgaatcatt atgcaggccc
     9361 cttccagttg atggttagcg gattacgggc aaaagataat gatgatcgaa aagatgccaa
     9421 tggtgatctc attcaaactg atgctgcaaa tactggcgta catgcgttag ttggtctgca
     9481 caatgacact ttctatggcc tgcgtgaagg gacggcaaaa acagcactgc tatatggcca
     9541 tggcctgggt gcggaagtca aagggattgg ctccgatggc gccctgctgt ctgaggcgaa
     9601 tacctggcgc ttcgcatctt acggcacaac acctctggga agcggttggt atgtcgcacc
     9661 agcaattctc gcacaaagca gtaaagatcg ttacgtcaaa ggcgatagct acgaatgggt
     9721 gaccttcaat acacgtctga tcaaagaggt aacacagaat tttgctctgg cctttgaggg
     9781 tagctatcaa tatatggatc tgaaaccaaa ggggtatcaa aaccacaacg ccgtaaacgg
     9841 cagtttctat aaactcacct ttgctccaac tctaaaagct aacgatatca ataatttctt
     9901 tagccgtccg gagcttcgcc tgtttgccac ctggatggac tggagcagca aacttgatga
     9961 ttttgccagc aatgacgctt tcggcagcag tggtttcaat actggcggag agtggaattt
    10021 tggtgtccaa atggaaacct ggttttaata ccagacaacc tcacagatgt gaagttttta
    10081 ctgaaataac aatgaattag agggttatat ggatttcgat aaaatcgccc aatcgttgct
    10141 tcctttatta ggtggtaagg agaacatcgc cagtgcagca cattgcgcca ctcgcctacg
    10201 gctggtactg gttgacgaca cacttgccga tcaacatgcc attggccaga ttgatggagt
    10261 gaaaggttgt tttcgtaatt cagggcaaat gcaaattatc tttggcaccg gcgtggttaa
    10321 taaagtctat gctgccttta ttcaggttgc aggtattagc gaatccagta aagcagatat
    10381 cgctcgactc gccgctcaaa aactcaatcc ttttcagcga atagcacggc tactttctaa
    10441 tatttttgtc cccatcattc cagccattgt tgcttctggt ttattaatgg ggcttctggg
    10501 aatggtgaaa acatatggct gggtgaatac cgataatgcg atttatatcc tgctggatat
    10561 gtgcagttca gccgcattta ttattctccc catccttatt ggctttactg ctgctcgtga
    10621 atttggtgga aatccttatc ttggcgcgac attaggaggg attctgactc acccggcact
    10681 cacaaatgcc tggggcgttg ctgcgggatt tcagacaatg aacttcttcg gttttgaaat
    10741 cgccatgatt ggctatcagg gaacggtttt ccccgtgctt ctggcagtat ggtttatgag
    10801 catagtggaa aaacagttac gcaggtttat ccctgatgct ctggatctca ttctgacgcc
    10861 atttctgact gtcgtcattt ctggctttat cgctcttttg attattggtc cggcaggacg
    10921 agcgttaggt gacggtatct ctttcgttct cagcacgctg attgcacacg caggctggtt
    10981 agcagggttg ttattcggcg ggctatattc agcgattgtt attacgggta ttcatcatag
    11041 cttccacgca attgaagcag gacttttagg taaccctgca atcggtgtta atttcctgct
    11101 gcctatttgg gcaatggcaa acgttgcgca aggcggtgca tgtctggcgg tatggtttaa
    11161 aaccaaagat acaaaaatta aagcaatcac cctaccctcg gccttttccg caatgttagg
    11221 gattactgaa gccgctattt ttggtatcaa ccttcgtttt gttaaacctt ttattgcggc
    11281 cttaattggt ggcgctgccg gcggtgcctg ggttgtctct gttcacgttt atatgactgc
    11341 cgttggtctg accgcaatac ccggcatggc aatcgttcag ccaacatcgt tggttaatta
    11401 tattattgga atggtgattg ctttcgctgt cgcttttagc ctctctttat tattcaaata
    11461 caaaacggac gaggagtaat gtcgatgcct tcacaaatgc ccgcgatttt acaggcagtg
    11521 atgaaaggtc agtccaaagc actggcagat gcccactatc cttgttggca tctcgctccc
    11581 gtcaccggac taatgaacga tccaaatggc ttttgctggt ctggaggccg ctatcatctg
    11641 ttttatcagt ggaatccact ggcttgtgat cataagtaca aatgttgggg gcactggagt
    11701 tcgacggatt tattgcactg gcagcatgaa ccattagcgc taatgcccga taaagagtac
    11761 gaccgtaatg gttgttactc aggaagcgcg gttaataatc agggcgttct cacactttgc
    11821 tataccggta atgttaagtt tgatgatggc agcagaacgg catggcaatg tctggccacc
    11881 gaaaataacc agggcgggtt tgataaacta ggccctgtca tatcgttacc tgatggttac
    11941 accggacatg ttcgtgatcc aaaagtctgg aagcacaaca gccaatggta catggtgctt
    12001 ggcgcgcaag ataaagagaa acgggggaaa gtgctgctgt attcctctgt cgatctgaat
    12061 acctggtctt ttcatggaga aattgctggt aacggtctga atgaaataga taacgcagga
    12121 tacatgtggg aatgtcctga tcttttcgcc cttgatggtg aatacattct cctctgttgc
    12181 ccccagggaa tggtgcgcga acatgagcgc tatctgaata cttatccctg cgcctggcta
    12241 catggacagt ttgattacga gacaggcaaa tttacgcatg gcgctttctc agagctggat
    12301 gcgggatttg aattctatgc accacagacg atggaagccc cggacgggcg acgattgctg
    12361 gtcggctgga tgggggttcc tgatggcgaa gagatgttgc agccaaccag aaagcatcat
    12421 tggcagcatc aaatgacctg ttttcgcgaa ctctcattcc agaagggaaa attgtttcag
    12481 atgccaatca gggaactcgc gcaactgcgt gaagctgaac acttctggca aggaaaagcg
    12541 gatcatgcgc cgcatgtcga aattgaacgc ctggaaatgg acatcattcc gtcgggtgag
    12601 ctgtatctga attttggcaa tgcgctggca ctgcatctta atgacgatgg tattcaacta
    12661 cagaggagaa gccttgcagg gcaagagaaa ctaactcgtt actggcgagg aagcgtaacg
    12721 tcactgaaga ttctctgtga cagttccagc gtagagatat tcatcaataa tggtgaaggt
    12781 gtaatgagta atcgttattt tccccatcat ccagcttcgc tgatattaca gggtgagtct
    12841 gacgtcacgt tacgctactg gtcgctacgc gcctgcatgg tagaatgagg gttttgaatt
    12901 acggatcctc gtcgtgagaa aaaccaaacg cgttactatt aaagacattg ctgaactggc
    12961 gggagtgtcg aaagcaaccg ccagtctggt tctcaatggt cgtggcaaag agttacgtgt
    13021 tgcgcaggaa acgcgtgagc gtgtgctgag tattgcccaa cagcagcatt atcagcccag
    13081 cattcatgcc cgctcattac gggacaaccg cagtcatacc attggtctcg tcgttcctga
    13141 aatcactaac tacggatttg cagtattttc ccatgaactg gaaacattgt gtcgggaagc
    13201 gggcgtccaa ctcctcatat cctgtactga tgaaaatcct tcacaggaaa caatggtggt
    13261 gaacaatatg attgcccgcc aggttgatgg gttaatcgtc gcatccagta tgctgcatga
    13321 taatgactat caaaaactta gcgagcaatt acctgttgtg ttgttcgatc gccacatgaa
    13381 cggcagcaca ttgccattgg tcattactga ctctgtttct cccaccgccg ctctggtggc
    13441 agatattgca cggagtcacc cggatgaatt ctattttctg gggggacaac cccgtttgtc
    13501 accgactcgc gatcgactgg aaggatttac acaaggcttg cagcaagccg gagtaacgct
    13561 acaacctgaa tggattattc acggtaatta tcatccgagc tcaggctatg aaatgtttgc
    13621 cgcgctatgc gctcgtttag gacgtcctcc taaagcctta ttcactgccg cttgtggatt
    13681 actggaaggc gttctgcgct atatgagcca atataattta ctcgacagta caatccacct
    13741 ggcgagtttt gatgatcatt atttgtatga ttcactttcg gtaagaatcg acacaataca
    13801 acaggataat cgccaacttg cttttcactg ctttgagctt atttcgcaac tgattgaagg
    13861 tgaaacgcca tccccattac agcgttatct tcctgccagt ttacaaaaac gctatagata
    13921 atttattaat tatcttatgt agctgagtat taggtgacaa atgatcagtc tttcccacga
    13981 caattgtatc gttctgatga actcatattg acgcattttt cgggcgtatc agcccccggt
    14041 gttacgaatg gtgcatacga acgatcgcag tttgacgatg gttgatttat tttgttgcgc
    14101 aggggcaatc gcctgctagg taccgaaggc tggctctgaa gtactgagtc gggtgatcgc
    14161 gtctggccgc tggctgcgaa ttactgacac gggattggtg gcaggtgatg aaatcctgtc
    14221 ccggaaatgg ctccggattg attcaagttc tgacccgctg tcagcccgcg tggttgctgc
    14281 tctggctcat ttcctgtcgt cagttttctc atgcccggct gcgggttttc ggactgaatg
    14341 ataatatctg acgacaggag tcgctcatct atcgaaggta aatctccctg aaagtaatag
    14401 ttctggcagg gggaacagaa ctgtaatcat tgtgccgtaa tttgagttgg ctacctttgc
    14461 tacatgaaaa ctttattcac tttgttcatg ttgattgggt ttttcaatga ggaaagtttc
    14521 cagggtacaa tctccattaa cctgtttccg caaagcaagt ggcattttac cgttaccaga
    14581 ccacgtcctg gtttcgccgt tctcatcctc aaagatatat ttcgcttttc tcacaccgcc
    14641 tttgggtttt cgtttggttt ccgttgtccc tgcaatagca gaaactggac cctgccattc
    14701 ttccgggtca agccccacag aaacgatgta ctcaaatacc tgttgacatt tagtttccag
    14761 ttccttacgt tcagccgctt ttcgttcagc atcttcacgg cgttcttcaa taacagtgtt
    14821 gagcttttcc tgcatgtcga gtaattgctc aaaatcaagt tcgcgtacat acgtgcgtag
    14881 tgaacgaata ttcgtcattg tccgactgat gatgctgtat tcttcttctt tagtcagttt
    14941 gcacataatt cccctctctt ttgcaataaa cgtgttttct ctggtgggtt gattacccag
    15001 tcttttttac tgtgtctgac attttgtact gtggtgcagt tgtaatgcaa accttccggg
    15061 cttctgggac acagcatttc agtccgggcc ccatcattac cattttgttg attcgaaacg
    15121 aaaaccctca ccaatatcga tcgattttac cgatcgatat gatcacatcg atcggttgta
    15181 accattttac cctgtctttt gcatggtttt aaaataggca accctctttg tcgggtaggg
    15241 caagaaccaa cggagatgat tgaaatgaaa aaggttattg cggtttcagc gcttgcaatg
    15301 gcaggcatgt tttcggccca gactctggct gatgagagca aaacaggctt ttatgtgacc
    15361 ggtaaagcag gtgcttctgt tatgtcactt tcagagcaac gtttcgttga tggtgaaggg
    15421 gcatgggcgg ataaatataa aggcggtgac aaaagtgata cggtttttgg cgcaggcctt
    15481 gcggtgggat acgattttta tcagcactac aatgtgccgg tgcgtactga agtggagttt
    15541 tatggccgcg gtaacgctga atcaaaatat cgtctgagtt attgggaaag tgtcggtggt
    15601 gccgagtttg atgatgcaca gaacaagctc agtgtaaaca cgctgatgct gaatgcgtat
    15661 tatgatttca gaaacagcag tgcattcacg ccatggatat ctgctggtct gggttatgct
    15721 cgggttcatc ataaaacttc gtacatatat accgataaca gtccggcagg cagtgaagta
    15781 tattcagctt cagcgtcaaa atacgaaaat aacctggcat ggagccttgg tgtcggtgtt
    15841 aaatacgatg tgacgcagga tttcagcctt gacctcagtt accggtatct ggatgcaggg
    15901 gattccactc tgacttataa agatgaggat ggcgctaaat ataaatcctc tgttgatgtc
    15961 agaagtaatg agtttatgtt aggtgcaaca tataacttct gatggccatt tctgcctgcg
    16021 ctttggagca cagtaaagtg caggtatgac aaatcaataa cagatatcct gatgatattc
    16081 tccctctggc aactaataat ttcagatgaa tgaaatataa atagagcaaa aatattactc
    16141 tcgttgattt tttacagggg gcaggtagcg cgcagtatgg ttgtcagtct gttaaatgaa
    16201 aaaggactgt tgttgggaca cgttgttttt tgtcatatca gcccttccgg aaacaataaa
    16261 ccgtattacg aaggaggctc ccggacatct ggtttaaagt ttgaacataa tcaaataaaa
    16321 atctgttgcc ggagggcgta gttttgtgga actgacaaat gatgtcagga gtatttatat
    16381 aattcactta cacaagagta atactacagg tgcagtgatt tatggctgac tcaacgagaa
    16441 atcgctcagc ggagcgtttg attgatatcc ttgttgaatt acagaattat ggcgtggtca
    16501 gtcgtcataa tttgatgaaa aaatacaaca tcactgagcg aacagcatat cgtgatctga
    16561 atatgctttc tccctttatt gaagcctgtg gcgatggaaa ataccggctt atttcagcaa
    16621 gagcaggaaa ccagagcaaa ggatcactgc acaaatctct ggccaggtta ctggatactg
    16681 atgcgatttt tcctgaacgg gatgaaggtt tctggcagaa attagaaaac agggcaaccg
    16741 aaaagcatat tcgtgttcag tttcataacc ctgagcacac tatcagggat gatttaagaa
    16801 aatatcttga tgttcttgaa aaagcgatcc gcaacagtaa tatttgtcag attgcatatg
    16861 ccggtaagac cagaatcgtt catccctata aattaactaa tcagagaagt atatggtatt
    16921 tgctggctac tgaagagaac aagttaaagt cgttttcatt agctaaaata aaatggctgg
    16981 atataaaaaa agaaaagttt gcaaagagtg aggaaataca gtcactgata agtgagtcat
    17041 gtgacccgtg ggtgagcgac aaaacatttg atgtagtgtt gttcattcac agtaatatcg
    17101 cccattattt tttgcgacgg gacttacttc cttaccagca attgctacac agacatgata
    17161 atggcattac gctgtcatgc aaggctgcac ataaaaatca aattatcccg ttgattttat
    17221 actggctacc gaatgttgaa attattgaac cagtatggtt gaaagaagcc gtgttaacaa
    17281 tgttaggaaa atacctcacc gctgagaatg tttcctgaaa tatttcagtt gactgatgat
    17341 aaagcaacct gtacaggagg aaaagaatgg cgttaccttt cattattttg ggtgtggctg
    17401 ccggcgctgc ggcattcggc ggaaaaaaag cttacgatgg atatcaaaaa aagtcagaag
    17461 cagaggatgt gctcgaaaaa gcaaaagagc gttacgagga gcataaagga aaatttgagc
    17521 aggctgaagc tcagacatca tcaaaattaa caacgctcgg aaataaagag cttgagattg
    17581 gtaaagattt cagtgagttc gacaggattg ctgctgagct cctggcgcgt ctggaaaaag
    17641 agggcaataa agacctgaaa ctgagtgtcc cgcaacataa catcaataag attcataacc
    17701 tggccatttc agcaacagaa tatctcagta cggtcgtagc tgccggagtt tccggggctg
    17761 ctgcgggttt tgctgtgtat agcggagtga tggcatttgc tgcggcttcc acaggtacac
    17821 ccattgcagc gctttctggt gccgcggctt ataacgcgac gatggcggca attggagggg
    17881 gctctctcgc ggcagggggc atgggaatgg ccggcggcgc aatggtatta ggcaccgcag
    17941 tggctgcacc gttgattgcc attgcgggct gggcttacga cagacacgcg acgaaagctc
    18001 ttgaaaatgc ccatcagtgt gcctgcaaag ttgaacgggc agttaagaaa atgaatctgg
    18061 cccgggagca ttttgcaaaa gttaatcaat acgttgatga aattctcgct gcgctgaaca
    18121 gaatgcatgc tgtatttatt aaacactatt ttgagccgct taaatccatg cattctgtta
    18181 ttacagaaca acaggatatc aatgtatcag atgaaatcat tatgcttatt gaccatggat
    18241 attcactggc cgctattatg acggatatta ttacaacgcc actgtttaaa ccgcggaaac
    18301 aggaaaatgg tgaggctgtg ataaaagata acgttattga aatggaaaag gatgataatg
    18361 gaatgaatgt tattaataag gaagagctgg atgatgtact ggccagttcg gttgtgaaat
    18421 ttgatgattt ttcatcccgg agttaatgat ttaccgggcg ttaaagcccg gctttacaag
    18481 aagtgaaata acatgaatgt ggaaaaattt gactgggcag aagagttaca tcagaccatg
    18541 gtgaagagcc tggttgcatc attcgggctg gattttctgt tgctaaatga tcgtaagggt
    18601 ggtgatgttg acactatcca taatgtgcgt aatggcattt atgccacgga ggctgagcga
    18661 cagcggtatg aaggtcgtgg cgactatgat tctcatcatt accacagcca tgagaactac
    18721 atcgcgacga accggaaagg aaagcgtgca catcagtcag ggactcttac tgatgcatat
    18781 acaggcgaag tgtttgcttc agacgataaa aaaaatctgg atcatattat ttccgctaaa
    18841 gaaattcatg atgatccggg gagaattctt gctgaacggg atggtgcaga actggcaaat
    18901 gacgcttcaa atctggcatt cactcatgag tcgctcaata aatcaaagaa agcagactca
    18961 atggatgcgt ttatttgcac gctgcagaaa aacagagaag atacgttaag gcagatagca
    19021 gagcttgaat ctcaggcaac actgacagaa aaagaaaaga aacgtctgat aagtctgaga
    19081 aaaaaagctg atgcagatac tgagcgtatg gaaagggctg ataaatatgc ccgggaaaaa
    19141 tacgagacca cgattaatca gagctattac ctgagtagta agtttgctac tgctgtcgcc
    19201 agtgcttcac ttaattccgg ttttcgtatg gggacgcgac agatgctggg gcttattctt
    19261 gccgagatct ggttcgaact gagggaacga atcccgcata ttatcagaca gcaacggaac
    19321 tcattctgcg ccggcaaatg cctgtccgaa ataacgtcag cattacgtgc ctgccaggaa
    19381 cgagtacagg aaaaattcag ttctttttta attgccttta aagatggtgt catcggaggg
    19441 atactgtcag gtattaccac aacgctgtta aatatattcc tgacgacgga aagaatgatt
    19501 gtccggctta tcagggaaat gtggaacaac ctcgttcagg cctttaaagt cctggtattt
    19561 aacccggagg gattatcgcc ggggcagtta gccaaagcgg tcactaaact gatagcagca
    19621 ggtgtggctg ttgccggtgg ggtgatgata aatgaggcga tggcgcagat ccttattttt
    19681 ccatttggtt cagagcttgc tgccttttgt ggtgcgctga cgacaggtat tctcgggctg
    19741 gtgatgaatt attatctcga atatagcgag gttatgcagc gggtatgggc atttctggac
    19801 aaatttaagg acaaattcca gcggtcactg gaatactttc aggcagtcaa tgctgaactt
    19861 gatcgctatc ttgtggaact tactgcactg gaattttctg ttgatgctgc cgcgttaaat
    19921 catttttcac ttcatctgga tagcgtaaac agtgagatag agcgcggact tctgctgaga
    19981 gaagaagtca gccgacgagg gattgcgttg ccgtttgagt caggaaatgt ggcttctgtt
    20041 cgtgactggt taaataagcg atgaaattac accctccatt tccctgtaac cagtgtgggg
    20101 cctgttgtcg tcatgtcaac agggcgggtg aaacgcaatg tctggatcgt ggtgacggta
    20161 tttgccgtca ctatcagact gattctcatt tgtgtgcaat ttatgataaa cgcccacaga
    20221 tttgccgggt tgaagaccag tatttactga attatcagtc tcagtacagc tggcaggagt
    20281 ttattgcatt aaaccgggcc gcctgcctta ttctgaataa actgtaatgc cattaatggc
    20341 ggcgtcagga tagaaactga gagaacataa aacagtaaaa tgtcattccg ttcagcagcc
    20401 agcgttgctt tctccggctg ctgttattgt aaggcatttt cgttaagaac gcagaaggag
    20461 aggatcgtga gattgctgtt aaaagggctc gtcggaatgg cgctggtctg gcttgttatt
    20521 ggtttcggga tggagttatt ttttctcgcc gatcgggttc atccggcatt aagagtaatc
    20581 gttggcctgg ggatgttatt cctgatgata atgattgcgg gcggtatgtg ccgttctctt
    20641 ctgtcacgaa agaaaacatc atgacgctcc tgtttacgcc gctacatttg tggcggcttt
    20701 atgattattc aaaataatga attcacctgt cacggcatgt tcagcgacca gtgccggaca
    20761 gaaatatcgc ttttacacaa ttattatgaa aattaatctt ccgttcatac actggccgcg
    20821 gatttgcaat gataagtgcc tcgtgttatc tgtgggggtc tttactcttt ctaatctcct
    20881 ggtatcctca ggagcacatc tgatctaccg ggtgttattc tttatcctgg caattcttat
    20941 tattaaggcg atgaaatcgc ttacgctgcg ttttattctg gcctttccgt tcacattact
    21001 gaccgcggca gatatcagta tcagcctgta ttcatggtgt acatttgaca ccacattcaa
    21061 tgatggtttt gcaatcagta tcctacagac agatcctgat gaagcaataa gaatgttcag
    21121 gatgtatgtt gtttatgtta tcgcatttat tatcctcttt ttattgttct tctgttcagc
    21181 aataaataaa acgtcctcat tgccatcgga aaaggtcacg gtaattacat ttttattgtt
    21241 aattacagta acgttatatt cttccttcca gtttgcgttg aagaaacagt atcaaataaa
    21301 tgaagttgat ccttacatag tcgcttcccg gtttgccaca tatacccctt tctttaatct
    21361 gaactatttt gcactggccg caaaggagca tcagagatta atgacgattg ctgataccat
    21421 acctcactac gatctggtga ttacggataa caacactgac gtatttgttc tggtgattgg
    21481 tgaatctgca aggactgata acatgtccat ttacgggtat tcgcgcccta ccacaccaga
    21541 gcttcagaaa cagaaatcac gactgaaact gttcactcag gcaatcagtg gcgcacctta
    21601 caccgcgctg gctgttccac tggcgttatc tgccgatagt gtactgcatc atgatatccg
    21661 taattaccct gataacatta tcaacatggc gaatcaggcg gggttcgata cctggtggct
    21721 cagtgcgcag tccgctttcc gacagaacgg aacggctgtg gccagtatcg ccatgcgggc
    21781 cagaaacaga atttatgtca gaggctatga tgaattactg ctcccacacc tggctgaagc
    21841 actgaacagt aatccgggga gcaggaaact gattgttctg catctgacgg gcagccatga
    21901 gccagtctgc agtaactggc ccagagataa agccgtgttt aaaccgctgg atacagaaga
    21961 agtctgctac gataattcca ttcattatac cgacagtttg ttgggacagg ttttcaccat
    22021 gctggaaacg cgtcaggcat ccgtcatgta tttttcagat cacggtctgg agtatgaccc
    22081 gacaaaagaa catgcctatt ttcatggtgg aataaaaccc agtcagcagg cttatcatgt
    22141 tccgatgttt atctggtaca gcccgacact gggagagcat gtcgatcgcc agacggtgaa
    22201 cagcgttttt tccactgcgt ataatgatta tctgattaat gcatggatgg gcgtaaccag
    22261 accggctcaa ccgaaaacgc cggaggaggt gattacccgc tggcagggga aatccgtggt
    22321 ttttgatgcc agtcataacg tgtttgacta caaagtatta cgaaagaact ttaatgaact
    22381 ccaggaataa ttcgttaaag taatttacga ctctattctt ttttaaagga aactttcaga
    22441 aaatgccccc agggcgataa cctggggaag ggggactgtg ttattactgg tggataaatt
    22501 attacttgtt tgaaagatac actaatgcaa tatctattga tgctggatgc ttgagtgttt
    22561 cattcctgct gctgaaaacg gcaatatcca gggccagaca tattcaccgt taatatttct
    22621 gcatttctgt acaatttcct cagaaagtgt ctcatcaatt ctttcccgca acagcttctg
    22681 ctgtcttaaa atgctttcgc atatttcgaa atcatcttcc tgcatcagca tttcggcgca
    22741 tttaatgatt tctgctcgtt catgcagcac agaggaatgg ttatagagcc agtttacaaa
    22801 cagcaatgct tccggatatt gtccccataa tggtgagtat gttgcatagt aatcttctgc
    22861 cgtatc
//
LOCUS       DAJOWB010000073        22625 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJOWB010000073 DAJOWB010000000
VERSION     DAJOWB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.073"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..440)
                     /locus_tag="OHO85_003013"
     CDS             complement(<1..440)
                     /locus_tag="OHO85_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCQ2672358.1"
                     /translation="MLAKDKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLG"
     gene            complement(450..1001)
                     /gene="ybcL"
                     /locus_tag="OHO85_003014"
     CDS             complement(450..1001)
                     /gene="ybcL"
                     /locus_tag="OHO85_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCQ2672359.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(1170..1502)
                     /gene="emrE"
                     /locus_tag="OHO85_003015"
     CDS             complement(1170..1502)
                     /gene="emrE"
                     /locus_tag="OHO85_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCQ2672360.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(1846..2160)
                     /gene="fliE"
                     /locus_tag="OHO85_003016"
     CDS             complement(1846..2160)
                     /gene="fliE"
                     /locus_tag="OHO85_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCQ2672361.1"
                     /translation="MSAIQGIEGVISQLQTTAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2375..4033
                     /gene="fliF"
                     /locus_tag="OHO85_003017"
     CDS             2375..4033
                     /gene="fliF"
                     /locus_tag="OHO85_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCQ2672362.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVTGSAAVAVMVALILWAK
                     TPDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEALTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREISDNDPRVVALVIRQWINNDHE"
     gene            4026..5021
                     /gene="fliG"
                     /locus_tag="OHO85_003018"
     CDS             4026..5021
                     /gene="fliG"
                     /locus_tag="OHO85_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCQ2672363.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLCHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5014..5700
                     /gene="fliH"
                     /locus_tag="OHO85_003019"
     CDS             5014..5700
                     /gene="fliH"
                     /locus_tag="OHO85_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCQ2672364.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5700..7073
                     /gene="fliI"
                     /locus_tag="OHO85_003020"
     CDS             5700..7073
                     /gene="fliI"
                     /locus_tag="OHO85_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCQ2672365.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGNGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7092..7535
                     /gene="fliJ"
                     /locus_tag="OHO85_003021"
     CDS             7092..7535
                     /gene="fliJ"
                     /locus_tag="OHO85_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCQ2672366.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7532..8659
                     /gene="fliK"
                     /locus_tag="OHO85_003022"
     CDS             7532..8659
                     /gene="fliK"
                     /locus_tag="OHO85_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCQ2672367.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQVRVTGNSGVDIFA"
     gene            8764..9228
                     /gene="fliL"
                     /locus_tag="OHO85_003023"
     CDS             8764..9228
                     /gene="fliL"
                     /locus_tag="OHO85_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCQ2672368.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            9233..10237
                     /gene="fliM"
                     /locus_tag="OHO85_003024"
     CDS             9233..10237
                     /gene="fliM"
                     /locus_tag="OHO85_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCQ2672369.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10234..10647
                     /gene="fliN"
                     /locus_tag="OHO85_003025"
     CDS             10234..10647
                     /gene="fliN"
                     /locus_tag="OHO85_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCQ2672370.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10650..11015
                     /gene="fliO"
                     /locus_tag="OHO85_003026"
     CDS             10650..11015
                     /gene="fliO"
                     /locus_tag="OHO85_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCQ2672371.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALITIIALILAAAWLIKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            11015..11752
                     /gene="fliP"
                     /locus_tag="OHO85_003027"
     CDS             11015..11752
                     /gene="fliP"
                     /locus_tag="OHO85_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCQ2672372.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11762..12031
                     /gene="fliQ"
                     /locus_tag="OHO85_003028"
     CDS             11762..12031
                     /gene="fliQ"
                     /locus_tag="OHO85_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCQ2672373.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12040..12825
                     /gene="fliR"
                     /locus_tag="OHO85_003029"
     CDS             12040..12825
                     /gene="fliR"
                     /locus_tag="OHO85_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCQ2672374.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            13115..13738
                     /gene="rcsA"
                     /locus_tag="OHO85_003030"
     CDS             13115..13738
                     /gene="rcsA"
                     /locus_tag="OHO85_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCQ2672375.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13782..13970)
                     /gene="dsrB"
                     /locus_tag="OHO85_003031"
     CDS             complement(13782..13970)
                     /gene="dsrB"
                     /locus_tag="OHO85_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCQ2672376.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14133..14360
                     /gene="yodD"
                     /locus_tag="OHO85_003032"
     CDS             14133..14360
                     /gene="yodD"
                     /locus_tag="OHO85_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCQ2672377.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14659..15474
                     /locus_tag="OHO85_003033"
     CDS             14659..15474
                     /locus_tag="OHO85_003033"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HCQ2672378.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLTRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(15471..17165)
                     /gene="yedQ"
                     /locus_tag="OHO85_003034"
                     /pseudo
     CDS             complement(15471..17165)
                     /gene="yedQ"
                     /locus_tag="OHO85_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(17336..17518)
                     /locus_tag="OHO85_003035"
     CDS             complement(17336..17518)
                     /locus_tag="OHO85_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCQ2672379.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(17597..18514)
                     /locus_tag="OHO85_003036"
     CDS             complement(17597..18514)
                     /locus_tag="OHO85_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCQ2672380.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKAFSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            18687..19607
                     /gene="yedA"
                     /locus_tag="OHO85_003037"
     CDS             18687..19607
                     /gene="yedA"
                     /locus_tag="OHO85_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCQ2672381.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSHITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(19596..20066)
                     /gene="vsr"
                     /locus_tag="OHO85_003038"
     CDS             complement(19596..20066)
                     /gene="vsr"
                     /locus_tag="OHO85_003038"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCQ2672382.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(20047..21465)
                     /gene="dcm"
                     /locus_tag="OHO85_003039"
     CDS             complement(20047..21465)
                     /gene="dcm"
                     /locus_tag="OHO85_003039"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCQ2672383.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISGCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRCSR"
     gene            complement(21532..22227)
                     /locus_tag="OHO85_003040"
     CDS             complement(21532..22227)
                     /locus_tag="OHO85_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCQ2672384.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLCEEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDAEDPLAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTVRGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(22267..22569)
                     /locus_tag="OHO85_003041"
     CDS             complement(22267..22569)
                     /locus_tag="OHO85_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCQ2672385.1"
                     /translation="MEYGSTKMEERLSRSPGGKLALWAFYTRCGYFVWAMARYIWVMS
                     RIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         5562 a         5943 c         5725 g         5395 t
ORIGIN      
        1 cctaatgaaa gtagaacgcc cattgctaag tgttcctgaa tttgcgttga gaagcctcgg
       61 gaaatagatg tttttagttg ctcccaacaa tatattaact cggggttctg aggtaagcag
      121 aaacctgtcg tgttacgaat ttgatcagtt atcgcataaa atttttgaaa cttttcaatt
      181 agatcaatgg gataataaag catttctgca agataaagac ctgcttctgg ataattcgca
      241 atataaaatt catatccaca gggaaataat attatctgat tattatcaac agttagacta
      301 tgcgtctccc aattgataac tttttttccc tgacggatac gacataaagc tggcataaga
      361 ggcttaaccc tgtgaatctc atgatgttta tgcatccgta tttcttcaat ctttaggtta
      421 gtcttatctt ttgccagcat actcttaccc tactttatct cataaactgg tgttatctca
      481 gcggttgcaa ttttattggc attaagcata taaccaacta acgctccgct ggaattagaa
      541 tccacaggaa ttttatcagt ttttagagcc catactttaa actggtaatg atggggttta
      601 tctcctttgg gaggacatgc tccaccaaat cctgcataac caaaatcatt tcggccttga
      661 acagcaccag tcggaagttt tgtaccatca cgtcttcctg catcagcggg caaatatgtt
      721 acagttgctg gaatattagc aacagtccag tgccaccatc cactgcctgt aggagcatct
      781 ggatcataca cagttacggc aaagcttttg gtgccttcag gagcaccaga ccaagttaat
      841 gagggcgatg tattaccacc ttcacaccca aatccagaaa atacatgaga cgttgtaagt
      901 tgctctcctg tttttatttc attactagtg acctgaaatg ctgcggcctg cgcagaaaat
      961 gttatgaatg ccaatacact tgaaacgata agttttttca taaaaacctc attgttgtga
     1021 cctatcgtta ttttatttga tgtgccttga tctcattatg cataaaggcg caatgatcgc
     1081 gcaaatgcaa tcataattat aaaggtgccc cgaacattag ccataatatg cacaaatcag
     1141 attcctgtta tctcaaggat aattattttt taatgcggtg cacttcgtga caacaagtta
     1201 atgactaaca caccggcaca aatcaacatc atgcctatga tagctggcag gtccagtcgt
     1261 tgaccaaaaa atccccagga aagcaaacta atcagaacga taccgacgcc tgaccagata
     1321 gcataagcaa tccctgtagg tatataagcc agcgtctgag ctaataacca gaatgatgca
     1381 caatagcaaa taattgtgcc aacagagggc cataaccgtg taaacccctc tgaaaacttc
     1441 attaaggttg taccaatgac ctctgcaagt attgcgccac caagataaat ataagggttc
     1501 atagcatatt ctttcctgtt catactggag agaattgtac tacagtttga acacaactca
     1561 ccgttttcat cctggtgtat gtgtatccag ggggctctgg cgtggcaaga gataaaggca
     1621 ggaacaataa tgatcagaag gaaccccagg ctaacgaatg agttcactct tcctgggagc
     1681 aaagcgaaga caaaacatgt tatcgcgttt acggatcgtg gatggcagaa aactttcagg
     1741 atcaggtatc cattttaaac cagaaattga gtgagtttgc cccatccatg ccccacgctg
     1801 tgggatcgga tataataaac aagcatagtc aacaggttac aaaacctaca cctgcatgga
     1861 catcacttcc tgatacgccg ccaccagctt attacgcacc tgaatcccca tttgcataga
     1921 aactgaggct ttttgcatat cggtcatcac atcgtttagc gccacgccgg gctcaccgag
     1981 ggtgaatttt tctgcctgcg tgcgggcggc tgtttgtgta tcgcttatgc gatcgagcgc
     2041 ggcgtgcagc tgcccggcaa aactaattgt cggttgcggc agtgattcct gcgcacgcgc
     2101 actcatcgcc gtggtctgta actggctgat aaccccttca atcccctgta tcgctgacat
     2161 tttcatctcc cggataattt ctggtagcaa agcctaccag taagtcaata agacaaaggc
     2221 gctaaatagc aacaaaaaaa cgggtttatt ggcggataga aaaaaacgaa agcacaaata
     2281 atgggagcgt caatttttcg agtttgctga cccgggagtg agtcttgttc cactttgcca
     2341 ataacgccgt ccataatcag ccacgaggtg cgcgatgaat gcgactgcag cccagacaaa
     2401 atctcttgag tggcttaatc gcctgcgtgc gaatccgaaa attccattga ttgttaccgg
     2461 ttccgcggca gtggcggtca tggtcgcact gatcctgtgg gcgaaaaccc ccgactaccg
     2521 tacattattc agcaatcttt ccgatcagga tggtggcgca attgtcagcc aactgacgca
     2581 aatgaatatt ccttaccgct tcagcgaagc cagcggcgct attgaggttc cggcagataa
     2641 agttcacgaa ctgcgcctgc gcctggcgca acaaggtttg cccaaaggcg gcgcggtcgg
     2701 tttcgaactg ctcgatcagg aaaagtttgg tatcagccag ttcagcgaac aggtcaatta
     2761 tcagcgggcg ctggaaggcg agctttctcg taccatcgaa actataggcc cggtaaaagg
     2821 ggcgcgcgta catctggcaa tgccgaaacc gtctttattc gtccgtgaac aaaaatcccc
     2881 ttctgcatcg gtgacggtaa atctgttacc cggccgcgca ctcgatgaag ggcaaattag
     2941 cgctattgta catctggttt ccagcgcggt tgctggtctg ccgccaggaa acgtcacgct
     3001 ggtggatcag ggcgggcatc tgttaaccca gtccaatacc agcgggcgcg atcttaatga
     3061 cgctcagttg aaatatgcca gcgatgtcga aggccgtatt cagcggcgta ttgaagcgat
     3121 cctgtcgcct attgttggta acggtaatat tcacgcccag gtcacggcgc aactggactt
     3181 cgccagtaaa gaacaaacgg aagaacagta tcgccctaac ggtgatgaat ctcatgcggc
     3241 gcttcgttca cgccagctta atgagagcga gcaaagcggt tccggttatc cgggcggcgt
     3301 accgggggcg ttgtcgaatc aaccggcacc tgcgaataac gcaccaatca gcacgcctcc
     3361 ggcaaatcaa aataaccgcc agcagcaggc gagcaccacc agcaatagtg ggccgcgtag
     3421 cacacagcgg aatgaaacca gtaactacga agtcgatcgc accattcgtc ataccaaaat
     3481 gaacgtgggc gatgtgcaac gtctgtcagt cgcggttgtg gtgaattaca aaaccttgcc
     3541 agacggtaaa ccattgcctc tcagcaacga acagatgaag caaattgaag ctctgacccg
     3601 cgaggcgatg ggcttttctg aaaaacgcgg tgattcgctc aatgttgtta actcgccgtt
     3661 caatagcagt gacgaaagcg gcggagaact gccgttctgg caacagcaag cgtttatcga
     3721 tcagttgctg gctgccggtc gctggttgct ggtactgctg gtggcgtggc tgctgtggcg
     3781 gaaagcggta cgcccgcagt taacacgtcg cgccgaggcg atgaaagctg tacagcaaca
     3841 ggcgcaggcc cgtgaggaag tggaagatgc ggtggaagtc cgcctgagca aagacgaaca
     3901 actccaacaa cgacgcgcta accaacgtct gggggcagaa gtcatgagcc agcgtatccg
     3961 tgaaatatct gataacgatc cgcgcgtggt ggcgctggtc attcgccagt ggataaataa
     4021 cgatcatgag taacctgaca ggcaccgata aaagcgtcat cctgctgatg accattggcg
     4081 aagaccgggc ggcagaggtg ttcaagcacc tctcccagcg cgaagtgcaa accctgagcg
     4141 ctgcaatggc gaacgtcacg cagatctcca acaagcagct aaccgatgtg ctggcggagt
     4201 ttgagcaaga agctgaacag tttgccgcac tgaatatcaa cgccaacgat tatctgcgtt
     4261 cggtattggt caaagctctg ggtgaggaac gtgccgccag cctgctggaa gatattctcg
     4321 aaactcgcga taccgccagc ggtattgaaa cgctcaactt tatggagccg cagagtgccg
     4381 ccgatctgat tcgcgatgag catccgcaaa ttatcgccac cattctggtg catctgaagc
     4441 gcgcccaggc cgccgatatt ctggcgttgt tcgatgaacg tctgtgccac gacgtgatgt
     4501 tgcgtatcgc cacctttggc ggcgtgcagc cagccgcgct ggcggaactg accgaagtac
     4561 tgaatggctt gctcgacggt cagaatctca agcgcagcaa aatgggcggc gtgagaacgg
     4621 cagccgaaat tatcaacctg atgaaaactc agcaggaaga agccgttatt accgccgtgc
     4681 gcgaattcga cggcgagctg gcgcaaaaaa ttatcgacga gatgttcctg ttcgaaaatc
     4741 tggtggatgt cgacgatcgc agcattcagc gtctgttgca ggaagtggat tccgaatcgc
     4801 tgttgatcgc gctgaaaggg gccgagcagc cactgcgcga gaaattcctg cgcaatatgt
     4861 cgcagcgtgc cgccgatatt ctgcgcgacg atctcgccaa ccgtggtccg gtgcgtctgt
     4921 cgcaggtgga aaacgaacag aaagcgattc tgctgattgt gcgccgcctt gccgaaactg
     4981 gcgagatggt aattggcagc ggcgaggata cctatgtctg ataatctgcc gtggaaaacc
     5041 tggacgccgg acgatctcgc gccaccacag gcagagtttg tgcccatggt cgagccggaa
     5101 gaaaccatca ttgaagaggc cgaacccagc cttgagcagc aactggcgca actgcaaatg
     5161 caggcccatg agcaaggtta tcaggcgggg attgccgaag gtcgccagca aggtcatgag
     5221 cagggctatc aggaaggact ggcccggggg ctggagcaag gtctggcaga ggcgaagtct
     5281 caacaagcgc caattcatgc ccggatgcag caactggtca gcgaatttca aactaccctt
     5341 gatgcgcttg atagtgtgat tgcttcgcgc ctgatgcaga tggcgctgga ggcggcacgt
     5401 caggtgatcg gtcagacgcc aacagtggat aactcggcac tgatcaaaca gatccaacag
     5461 ttgttgcagc aagaaccgtt attcagcggt aaaccacagc tgcgcgtgca cccggatgat
     5521 ctgcaacgtg tggatgatat gctcggcgca accttaagtt tgcatggctg gcgcttgcgg
     5581 ggcgatccca cactccatcc tggcggctgt aaagtctccg ccgatgaagg cgatctcgac
     5641 gccagtgtcg ccactcgctg gcaagaactc tgccgtctgg cagcaccagg agtggtgtaa
     5701 tgaccacgcg cctgactcgc tggctaacca cgctggataa ctttgaagcc aaaatggcgc
     5761 agttgcctgc ggtacgtcgc tatgggcgat taacccgcgc taccgggctg gtgctggaag
     5821 ccaccggatt acaattgccg ctcggcgcaa cctgtgtcat tgaacgccag aacggcagcg
     5881 aaacgcacga agtagaaagc gaagtcgttg gctttaacgg tcaacggctg tttttaatgc
     5941 cgctggagga agtggaaggt gtcctgcccg gcgcgcgggt ttatgccaaa aacatttcgg
     6001 ctgaaggact gcaaagcggc aagcagttgc cgctcggtcc ggcgttatta ggtcgcgttc
     6061 tggacggcaa cggtaagccg ctcgatggcc tgccctcccc cgatacgacg gaaaccggtg
     6121 cgctgattac cccgccattt aacccgttgc aacgtacacc gattgaacat gtgctggaca
     6181 ccggcgtgcg cccaatcaat gccctgctta ccgttgggcg cgggcagcgt atggggctgt
     6241 ttgccgggtc cggcgttggt aaaagtgtgc tgctggggat gatggcacgt tacacccgcg
     6301 ccgatgtcat tgtcgtgggt ttgattggtg aacgtgggcg cgaagtaaaa gattttattg
     6361 agaatatcct cggtgccgaa gggcgtgcac gctccgtggt gattgccgct ccggcggatg
     6421 tctctccgct tctgcgaatg caaggtgccg cctatgccac gcgcattgcc gaagattttc
     6481 gcgatcgtgg tcagcatgtg ttgctgatta tggactccct cacccgctac gcgatggccc
     6541 agcgtgaaat tgcgctggcg attggcgaac cacccgccac caaaggttat cccccgtcgg
     6601 tgtttgccaa attaccggca ctggtcgagc gtgccggaaa tggcattagc ggcggcggat
     6661 cgattaccgc gttttatacc gtgctcaccg agggcgatga ccagcaggac cccattgccg
     6721 actccgcgcg ggcaatcctc gacggtcaca ttgtgctgtc tcgccgactg gcggaagccg
     6781 ggcactatcc ggctatcgat attgaagcgt cgatcagccg cgcaatgacg gcgttgatca
     6841 gcgagcaaca ttacgcgcga gtgcgcacct tcaaacagct gttgtcgagt tttcagcgta
     6901 accgagatct ggttagcgtc ggcgcgtatg ccaaaggcag cgatccgatg ctcgataaag
     6961 ccatcgccct gtggccgcag ctggagggct atttgcaaca aggcattttt gaacgcgcgg
     7021 actgggaagc gtctctccag gggctggagc gtattttccc gacagtgtca taacccagga
     7081 gataacggca gatggcagaa catggtgcgc tggcgaccct gaaagatctg gcagaaaaag
     7141 aggtagagga tgccgcgcgc ctgctgggtg aaatgcgtcg cggatgtcag caggcggaag
     7201 aacagctcaa aatgctgatc gattatcaga atgaatatcg caataacctc aatagcgata
     7261 tgagtgccgg gatgaccagc aaccgctgga tcaactatca gcagtttatc cagacgttgg
     7321 aaaaagccat tactcagcat cgccagcaac ttaatcagtg gacgcagaaa gttgacattg
     7381 ccctgaacag ctggcgagaa aagaaacaac gtttgcaggc ctggcagaca ctgcaggaac
     7441 ggcaatccac ggcggcactg cttgcagaaa accgcctcga tcagaaaaag atggatgagt
     7501 tcgcccagcg cgccgccatg aggaaacctg aatgattcgc ttagcgccct tgattaccgc
     7561 cgacgttgac accaccacat tgcctggcgg caaagccagc gatgctgcac aagattttct
     7621 cgcgttgttg agcgaagcat tagcaggcga gacaactacc gacaaagcgg ccccccagtt
     7681 gctggtggca acagataagc ccacgacaaa aggcgagccg ctggtcagcg agattcttgc
     7741 cgatgcgcaa caagcggatt tactgatccc tgtggatgaa acaccgcctg tcatcaacga
     7801 cgaacaatcc acatcaacac cattaaccac cgctcaaacg atgacgttgg ctgcggtggc
     7861 tggcaacaat acggcaaaag acgaaaaagc ggatgatctg aatgaagacg tcaccgcaag
     7921 cctgagcgcc ctttttgcga tgttgccggg ttttgacaat acgcccaaag tgactgatgt
     7981 gccgtcaacc gtgttaccgg cagagaaacc aacgctcttc acaaaactga cttctgcgca
     8041 actcacaaca gcacagcctg atgatgcccc cggcacgcca gctcagccat taacaccgct
     8101 ggtagcagaa gcccagagta aagcggaagt catcagcaca ccttcaccgg tgaccgctgc
     8161 cgccagcccg ctaatcactc cacaccagac acagccactg cccaccgtcg ccgcgcctgt
     8221 gttgagtgca ccgctgggtt ctcacgaatg gcaacaatca ttaagccagc atatttcgct
     8281 gttcacccgc caggggcaac aaagtgcaga gttgcgtctg cacccgcagg atttaggtga
     8341 agtgcaaatc tccctcaaag tggatgataa ccaggcgcaa atccagatgg tttcaccgca
     8401 tcaacacgta cgcgccgccc tggaagcagc gcttccggta ctgcgaacgc agctggccga
     8461 aagtggcatt cagttagggc aaagcaacat cagtggcgaa agctttagtg gtcagcagca
     8521 ggccgcttcc cagcaacagc aaagccaacg cacagcaaac catgaacctc tggcggggga
     8581 agacgacgat acgcttccgg ttcccgtctc tttacaagta cgtgtaacag gcaacagcgg
     8641 cgttgatatt ttcgcctaac gtcagaggta gcaccgtaat ccgcgtcttt tccccgcttt
     8701 gttgcgctca agacgcagga taattagccg ataagcagta gcgacacagg aagaccgcaa
     8761 cacatgactg attacgcgat aagcaagaaa agcaagcgat cgctttggat cccgattctg
     8821 gtattcatta ccctcgcggc ctgtgccagc gcaggttaca gctactggca ttcgcatcag
     8881 gttgccgctg acgacaaagc gcagcaacgc gtcgtgccct ccccggtctt ctatgcgctg
     8941 gataccttta cggtcaatct gggcgatgca gatcgcgtac tttatatcgg cataaccctg
     9001 cgcctgaaag atgaagctac ccgctcgcgg ctgagtgagt atttgccgga agtccgtagc
     9061 cgtttgctgt tactgttttc gcgtcaggat gctgccgtac tggcgacaga agaaggcaag
     9121 aaaaacctga ttgccgagat taaaaccacg ctttccaccc cgcttgttgc cgggcaaccg
     9181 aaacaggatg tcaccgacgt gctgtatacc gcttttattc tgcgataacg acatgggcga
     9241 tagtattctt tctcaagctg aaattgatgc gctgttgaat ggtgacagcg aagtcaaaga
     9301 cgaaccgaca gccagtgtta gcggcgaaag tgacattcgt ccgtacgatc cgaataccca
     9361 acgacgggtt gtgcgcgaac gtttgcaggc gctggaaatc attaatgagc gctttgcccg
     9421 ccattttcgt atggggctgt tcaacctgct gcgtcgtagc ccggatataa ccgtcggggc
     9481 catccgcatt cagccgtacc atgaatttgc ccgcaacctg ccggtgccga ccaacctgaa
     9541 ccttatccat ctgaaaccgc tgcgcggcac agggctggtg gtgttttcac cgagtctggt
     9601 gtttatcgcc gtggataacc tgtttggcgg cgatggacgc ttcccgacca aagtggaagg
     9661 tcgcgagttt acccataccg aacagcgcgt catcaaccgc atgttaaagc tggcgcttga
     9721 aggctatagc gacgcctgga aggcgattaa tccgctggaa gttgagtacg tgcgttcgga
     9781 aatgcaggtg aaatttacca atatcaccac ctcgccgaac gacattgtgg ttaacacgcc
     9841 gttccatgtg gagattggca acctgaccgg cgaatttaat atctgcctgc cattcagcat
     9901 gatcgagccg ctacgggaat tgttggttaa cccgccgctg gaaaactcgc gtaatgaaga
     9961 tcagaactgg cgcgataacc tggtgcgcca ggtgcagcat tcacagctgg agttggtcgc
    10021 caactttgcc gatatctcgc tacgcctgtc gcagatttta aaactgaaac ccggcgacgt
    10081 cctgccgata gaaaaacccg accgcatcat cgcccatgtt gacggcgtcc cggtgctgac
    10141 cagtcagtac ggcactctca acggtcagta tgcgttacgg atagaacatt tgattaaccc
    10201 gattttaaat tctctgaacg aggaacagcc caaatgagtg acatgaataa tccggccgat
    10261 gacaacaacg gcgcaatgga cgatctgtgg gctgaagcgt tgagcgaaca aaaatcaacc
    10321 agtagcaaaa gcgctgccga cgcggtgttc cagcaatttg gcggtggtga tgtcagcgga
    10381 acgttgcagg atatcgacct gattatggat attccggtca agctgaccgt cgaactgggc
    10441 cgtacgcgga tgaccatcaa agagctgttg cgtctgacgc aagggtccgt cgtggcgctg
    10501 gacggtctgg cgggcgaacc actggatatt ctgatcaacg gttatttaat cgcccagggc
    10561 gaagtggtgg tcgttgccga taaatatggc gtgcggatca ccgatatcat tactccgtct
    10621 gagcgaatgc gtcgcctgag ccgttagtga tgaataacca cgctactgtg caatcttccg
    10681 cgccggtttc tgctgcgcca ctgctgcagg tgagcggcgc actcatcacc attattgccc
    10741 tgatcctcgc tgctgcctgg ctgataaaac ggctgggatt tgcccctaaa cgcactggcg
    10801 ttaacggtct gaaaattagc gccagtgctt cgctgggcgc gcgtgaacgg gttgtggtgg
    10861 tcgatgtgga agatgcacgg ctggtgctcg gcgttaccgc agggcaaatc aatctgctgc
    10921 ataaacttcc cccttcagca ccaacagaag agataccgca gaccgatttt cagtcggtca
    10981 tgaaaaattt gcttaagcgt agcgggagat cctgatgcgt cgtttattgt ctgtcgcacc
    11041 tgtccttctc tggctgatta cgcccctcgc cttcgcgcaa ctgccgggta tcaccagcca
    11101 gccgctgcct ggcggtggac aaagctggtc gctcccggtg cagacgctgg tgttcatcac
    11161 ctcgttgacg tttattccgg caattttact gatgatgacc agtttcaccc gcatcatcat
    11221 tgtttttggt ttattgcgta acgcgctggg aacaccctcc gcgccaccta accaggtatt
    11281 gctggggctg gcactgtttt tgaccttttt tattatgtca ccggtgatcg acaaaattta
    11341 tgtcgatgcg taccagccat ttagcgaaga gaaaatctca atgcaggagg cgctggaaaa
    11401 aggggcgcag ccgctgcgtg agtttatgct gcgtcagacc cgcgaggcgg atttagggtt
    11461 gtttgccaga ctggcgaata ccggcccgtt gcagggacct gaagccgtgc cgatgcgcat
    11521 tttgctcccg gcctacgtga ccagcgagtt gaaaaccgca tttcagatag gcttcacgat
    11581 tttcattcct tttttgatta tcgacctggt gattgccagc gtgttgatgg cattggggat
    11641 gatgatggtt cccccagcca ccattgctct gccctttaaa ctgatgctgt ttgtactggt
    11701 ggatggttgg caattgctgg tcggttcgct ggcgcagagc ttttacagct agagaggcaa
    11761 aatgacacct gaatcggtca tgatgatggg gactgaagcg atgaaagtcg cgctggcact
    11821 ggctgccccg ctgttgctgg tggcgctggt cacgggtctt atcatcagta ttttgcaggc
    11881 cgccacccag attaacgaaa tgacgctgtc gtttattccg aaaatcatcg ccgtatttat
    11941 cgccattatt attgccggac cgtggatgct caatctgttg ctggattacg tccgcacctt
    12001 gttcactaac ctgccgtata tcatcgggta ggccgtacaa tgatgcaggt gacaagcgat
    12061 caatggcttt cctggttaag cctgtacttc tggccgttac tgcgcgtgct ggcgctcatt
    12121 tctaccgcac caattctgag cgaacgcagc gtgccgaaac gggtaaaact ggggctggca
    12181 atgatgatca cgttcgccat tgccccatca ttacctgcca acgatgttcc tgttttttcg
    12241 ttctttgctc tgtggctggc cgtgcagcag atcctgatcg gcattgcgct tggttttacc
    12301 atgcaatttg cctttgccgc tgtgcgaacc gctggcgaaa ttatcggtct gcaaatgggg
    12361 ctgtcatttg cgacgtttgt cgatccggcc agccatctca atatgcccgt tttagcgcgt
    12421 atcatggata tgctggcgtt actgctgttc ctgacattta acggtcattt atggttgatt
    12481 tcactgctgg tcgatacctt tcacaccctg cctattggtg gcgaaccgtt gaacagcaat
    12541 gcgtttctgg cactcaccaa agcagggagt ttgattttcc ttaacgggct gatgctggcg
    12601 ttaccgctca ttactctgct gctgaccctg aatctggcat taggtttact gaatcgtatg
    12661 gccccgcaat tatccatttt tgttattgga tttccattaa ctctgactgt cggtatctct
    12721 ttaatggcgg cattaatgcc gttaattgcg cctttttgcg aacatttatt cagtgaaatt
    12781 tttaatttgc tggctgatat tattagtgaa ttgccattaa tataattccg taacgtttat
    12841 catgttatcc taaggattat ccgaaaaata atacctacga acatcttcca agatactcct
    12901 gcagcgaaat atttgtttta agctcactca catatcgcaa catttacttt actttaagac
    12961 aattccaggc aaattataca acactttacg ggatagtaag tccgcctgaa aaatcgcgag
    13021 agtggcgcat taggtgaccc atgttgttcc gtttagtcat gatgaaatat tcaggtaagg
    13081 ggaattatcg ttacgcattg agtgagggta tgccatgtca acgattatta tggatttatg
    13141 tagttacacc cgactaggtt taaccgggta tctgttgagt agaggggtta aaaaaagaga
    13201 aatcaacgac attgaaaccg ttgatgacct tgccatagct tgtgattcac agcgcccttc
    13261 agtggtgttt attaatgagg actgtttcat ccatgatgct tctaacagtc agcacatcaa
    13321 gcacatcatt aatcaacatc ccaatacgtt atttatcgtt tttatggcaa ttgccaatgt
    13381 tcattttgat gaatatctat tggtcagaaa aaatttattg ataagttcta aatcgattaa
    13441 accggaatct ctcgacgata tccttggcga tattctgaaa aaagagacaa cgataacctc
    13501 gtttctaaat atgccgacgt tatcattgag ccgaaccgaa tcgagtatgt tgcgaatgtg
    13561 gatggcaggt cagggaacca ttcaaatctc tgaccaaatg aatatcaaag ccaagaccgt
    13621 ttcatcgcat aaaggtaata ttaaacgtaa gatcaaaacg cataataaac aggttatcta
    13681 ccatgtcgtc cgactgacgg ataatgtgac taatggtatt tttgtcaaca tgcgctaaca
    13741 cattctgact ggtggtttcc caccagtcag gctgaataag attactctgc tttctccaca
    13801 aagataccgt cctgatgccc tgcttcatta aaaaaccaga tgccgagcgg gtagtcttcc
    13861 agcgaaacca ggtacattgt gccttcacta aactcctcaa ctgccagtac cacgccagga
    13921 cgacgcggac cgccatccgt tttgactgtt acccgatcat tcaccttcat cgttttcctc
    13981 ctgtgccttt gtgccagtgt agaacaattt cgtttttctg gcagcgccag gcgcgcgcga
    14041 gtgctgattt tctcgacggt ctatacttaa gtgatgccag gcggacttaa cgactggcgg
    14101 caacaacaga gtaacggttg cgaggaaaga tgatgaaaac cgcaaaagag tacagcgata
    14161 ccgcaaaacg tgaggtcagc gtcgatgtcg atgccctgct ggcggcgatc aatgaaatta
    14221 gcgaaagcga agttcatcgc agccagaacg attctgaaca cgttagcgtc gatggacgtg
    14281 aatatcatac atggcgtgaa ttggcggatg ccttcgaact ggatattcat gacttcagcg
    14341 tctctgaagt gaatcgttga atgcacaata aaaaaatccc gaccctgagg gggtcgggat
    14401 gaaacttgct taagcaagaa gcacttaaaa aattcgttac accaggaaat ctgatgtgtt
    14461 catcacctta tccgcaattt ttttcgctga caagaaaata ttcgctatat gaatgattaa
    14521 cgctacttat gaatagaaat gtgacgcacg tcaccccttg gtggctctgg cgtcagcggg
    14581 atcgctcccg aacaaaatca tctttgataa tttgtgctgt tttgagaatc atcctggtaa
    14641 ggggagtact gcgcgactat gttttcaatt caacaaccac tactggtttt tagcgatctt
    14701 gatggcaccc tgctggacag tcatagttat gactggcaac cggcagcccc ctggctcacc
    14761 cgtttacgcg aagcaaatgt tcccgtcatt ctctgtagca gtaaaacatc agcggaaatg
    14821 ctgtacttgc aaaaaacgtt ggggctacaa ggtttaccgc tgattgccga aaatggcgca
    14881 gtgatccagc ttgctgagca atggcaggag atagacggtt ttccacgcat catctcaggt
    14941 attagccatg gcgaaatcag ccaggtttta aatacgctac gcgagaaaga acattttaaa
    15001 ttcacgactt ttgatgatgt cgacgatgca actatcgccg aatggacggg attaagccgt
    15061 agccaggcgg cgctgacgca gcttcatgag gcgtcggtaa cgctaatctg gcgcgacagt
    15121 gacgagcgta tggcacaatt taccgctcgt ctgaacgaac tgggcttaca gtttatgcaa
    15181 ggtgcgcgct tctggcacgt cctggatgcc tctgccggaa aagatcaggc tgccaactgg
    15241 attatcgcga cctatcaaca attgtcaggc aaacgcccaa ccacacttgg cctgggcgat
    15301 gggccaaacg atgcgccctt actggaggta atggattacg cggtgattgt gaaagggcta
    15361 aaccgtgaag gggtgcatct gcatgatgag gatccggccc gcgtctggcg aacgcagcgt
    15421 gaaggaccgg aaggatggcg tgaagggctg gaccattttt tctccgcccg ttaagcgtta
    15481 tcgctcgcga atacccgatt acgcccagcc tgtttagcga gataaagccg acggtcggcc
    15541 agtgactgga attgttcaaa atcataatca ccggtttcct cgctgctact tacccccaac
    15601 gaggcactga tgcgtatcgt cgtactctta gcaatcaaca tctctttttc atttaacttc
    15661 aggcgaatac gttctgcgac ttccgcagcc tccgtcagac tcgcgcctgg cagaatcaca
    15721 caaaactcct caccaccgac ccgcccggca acgtcctgcg cacgcaagga actgctaatt
    15781 aatccggcag catgagaaag aacacggtcg cccgcctgat gaccaaagcg gtcattaatc
    15841 gctttaaaat ggtcaagatc gacctgaatg acagaaaaag gatgttggtg cgtctgacac
    15901 aatttagcga gcggacgggc tttttcgaac agtgcgccac gattatataa acgggttaag
    15961 gtgtcgtgcc acgcctgcca ctgcaacgaa ctttgcagaa catacatgtt gctgaccatc
    16021 cggcgaatca cataccagga gatgagtaac atggtggtaa agagcgccca cagcagggtt
    16081 aatgcaatgc tgatactgcc gaaatcgccg cgcacgcctt cgcttaacgt atggacgcgc
    16141 accagcacac cgtcgaaatg gtccagacgt tcccagctaa catagcgact gtccatacga
    16201 atgccgcccc gcgtgtcatg ttccattgcc tacgccagca aggccagttc acgaggatca
    16261 aaaatattcc ctgttggatg gtcaggattg gaagaggtca aaaatctcag cttactgtca
    16321 tagagctgat actcaccatc gaggttttta tcgatggcgt ttcttaaaaa ttgctgcata
    16381 gtacgcacgg gaatactcat ccccagcacg ccataccagt agttattact gtctaccgga
    16441 acactgacgg taacctgcgg ttcagtattg ctggcgtgtt ccggttgcga agtgaaccag
    16501 cgtaccgcgc ggtgacgatt ttctcgttgt gaatggccaa taaaccaggg ttgggtgaca
    16561 tagccgtaat aacgcgttgg aacattgcgc gtaaacaagg tcggctgcgt cgaaacgtaa
    16621 aatccggcac gcgagacata catcgcctgt tcaaccatcg acgaggagtt gtgcgctagt
    16681 cgcagcaagt aaccaacttc cagtgcagcg gtaatttcat tgtcgaggct ttcattttcg
    16741 cgagacagga gattcccctc gctgactaat gcatccgaca cgccattgac tggcagggtg
    16801 cgccgtcggt tgagttcgat tttccaggcg tgctcgtcgc gatgctgttc gaactcggtt
    16861 acagcatcac gcagagaggt gaaatccagt ggcgctatga gagcttcgcg catgccatta
    16921 cgcaaaaaga tcagtttgtc gacgttatac tgcaaatgct tatcgagcgc gttggctacg
    16981 ttttccagat gattacgctg gctggagata taggcatctt ccagcaccac tacttcacgc
    17041 caggtgagca aggtggaaaa aagcaatacc actataaagc agagattaac gatatgacca
    17101 ggccccaggc ggcgtgcgag ttttttcaac cagctctggt tttccatttt tgtctcgtgc
    17161 tgcaccctga cgactcccca cctgcttttt tatgattctg gcataacctg gcgatagcgt
    17221 accgcaaaac agcgataatt ttataatggt ggcatgataa gacgcgacta tcattcattg
    17281 caggcattgg cacaccaggc cggatgtgat ccggcctggt cttaatgata ccctttcaaa
    17341 cttcttccgc agaacgactc ccctcccctg gaacaagctc ggtttcatga aaagcttccc
    17401 gcttgacccc gtaaccgtca taccaacgac actcaaccat accgctggag tatccagtga
    17461 caatcatccg cgggccgccc tctttaaccg taacttcctc actaaccata aagctcatac
    17521 tcgcctcctt ttttcgagtg aaacttgttc accttagttg aagatggcga attttgcata
    17581 atagcttgtg cgagtattaa tgtgcctgac cgcgcatttt tgctacggct ttcactccca
    17641 gcaccacgat gccgccgatg ataaatccaa gaatcagatt taaaacagtc ggtaatatca
    17701 tcgccaccac tgcactttgc tgcccggcga aatgttcaat ggcgtgatgc agcggcgcaa
    17761 taccatgtac cacaatcccg ccgccgacga ggaacatcgc cagcgtgccg acaatcgaaa
    17821 acgctttcat cagccagggc gcgataatca ataatccttt acctaatgcc tgcatcagcg
    17881 cgctggattt ttccgccagc caatacccca ggtcatcaat cttaacgata acccctacca
    17941 gaccgtagac gcccacggtc actaccagcg cgatgcctga aagcaccagc acctgattaa
    18001 gcaacggcgc ttcggccaca atccccagcg tgatggcgac gatttccgca gacaatataa
    18061 aatcggtacg aatcgcccct tttattttgt ccttttcaaa cttcagcgga tcctgcgccg
    18121 ccagcttctc cagacgctgc tgactctgcg ccggatcttc tttatgttta cgcgcctcca
    18181 gcatatgcag cactttctct accccttcaa agcagagaaa cgcgccaccg atcatcaaca
    18241 atggcgtaat cgcccacggg ataaacgcac tgataatcag cgccagtggc accagaatca
    18301 ctttattgat cagcgatcct ttcgccacgc cccagaccac gggaagttcc cggttggccc
    18361 gcacacctga aacttgttgc gcattgagcg ataagtcatc ccctaataca ccggcggttt
    18421 tcttcgccgc cagtttgccc atcacggaga tatcgtccag cagtgtggcg atatcatcga
    18481 gcaacgtcag taaactactc ccggccaaaa gcattccctc tcatttttat tgttgaaggg
    18541 tgtaagtatg aagcaaaaac ggcagctctg atacttgctt cacaggtcaa cattttttta
    18601 acaatcaagt gcaattatat ctctcgccag cagaatgatt ttcacgttta ctatgaacca
    18661 cctttttatt tccaccgtga gggattatgc gtttccgcca gttattaccg ctttttggcg
    18721 cgctgtttgc gttgtatatc atttggggct caacctattt tgtcattcgg attggcgtgg
    18781 aaagctggcc tccattaatg atggcgggcg ttcgattcct ggcagccggt attttattgc
    18841 tggcattttt gctactgcgc ggacacaagc tccccccgct acgtccgctg ctcaatgccg
    18901 cgctgattgg cctgttattg ctggctgtcg gtaatggcat ggtgacggtt gccgaacatc
    18961 aaaatgttcc ttccggcatc gccgccgtag tggttgcaac cgtgcccctc tttaccctgt
    19021 gcttcagccg cctgtttggc attaaaacgc gcaaactgga atgggtgggt attgccattg
    19081 ggcttgccgg aatcatcatg ctcaatagcg gtggaaattt aagcggcaat ccgtggggcg
    19141 cgattctgat tttaatcggc tcgattagct gggcgtttgg ctcagtttat ggctcgcaca
    19201 ttaccttacc tgtagggatg atggcgggtg cgattgagat gctggcggca ggcgtggtgt
    19261 taatgatcgc gtcgatgatt gcgggtgaaa aactgacggc gctcccttcc ctttcaggct
    19321 tccttgcggt cggctatctg gcgctgtttg gttcgattat cgccatcaac gcttatatgt
    19381 atttaatccg caacgtcagt ccggctctcg ccaccagcta cgcctacgtt aacccggtgg
    19441 tcgcggtctt gctgggtacg ggactgggtg gagagacgct ttcgaagatt gaatggctgg
    19501 cgctcggcgt aattgtcttc gcggtggtgc tggtcacgtt gggaaaatat ctctttccgg
    19561 caaaacccgt agttgcgcca gttattcagg acgcatcaag cgagtaaatg aatcccctgc
    19621 gtgtcgatct gcgcgctggc accttcgccg cagatccact cttccagacg ctcgctaagc
    19681 gcttcatccg tcagcttctc gcgcccacgt aacgcgcact cccagacaat caatacgcgc
    19741 cagccgagtt cctgcaagcg actgatatcg cggcgatcgc gctcaacatt tttacctatc
    19801 ttctccagcc agaactcggt tcgagtcgca ggcactttaa acagatagca gtgatgatga
    19861 tgccagaagc agccatgggt aaatatcacg cagcgatatt catcaacgac aaaatccggg
    19921 cgtccgggca gactggcgtc ctgaacgcga aacgccagtc cctgcccggt taacaaactg
    19981 gcgaggcgct tctcaatcgc cgtatcacgc gtggcaatcg cgcgcatatt tttgctgcgt
    20041 gtggccttat cgtgaacatc ggccatgttg tgcctcttgc tgacgcaacg ccaccgcctg
    20101 tttgattttt ggctcaagca gttttgccac cgcggcaaag accggcacga ccaccgagtt
    20161 accgaactgg cgataggcct gagtgtccga aaccggaatg cggaatttcg cttctcccgg
    20221 cgcttcaaaa cccattaagc gcgcgcattc ccgaggcgtt aaccgacgtg gacgatgttg
    20281 ctgattcagc ggatcgtcaa agtctttttc acccgtggcc atatcccatc cgcgatcgat
    20341 taaaatttcc gcgccatctt tgtaataacg cgcagacagc gtacgggtga cgctttgcgg
    20401 attgttcgga taaaccattc cataaccgaa gccgttaccg cgcgcctgat gttttttcgc
    20461 atatcgatag aggtacttcc acagtaccgg cgtcaggata tatttcgcct cgaccatcgg
    20521 gtccagcagc tgcgccagcg tcactcgctg cgcagggaaa catccgctga tatcacgcag
    20581 agtaaaatcg gctttcaggt taagatcgcg acgaaaaccc accagcacga tgcgttcacg
    20641 gtgctgcggc agaaaatgtt taccatcgat gattttcgga tcgtccggcc cgttatcttc
    20701 tgcatcagcc acgtcatagc ccagttcgtc cagcgtctgc atgatgatgc ggaacgtttt
    20761 accctggtcg tggcttttca ggtttttgac gttttcgagc acaaacatcg ccggacgacg
    20821 cgcgtcgata atgcgcacca catcaaagaa cagcgtgccc tgagtatcgc aggcaaaacc
    20881 gtgcgcccgc ccgagcgagt tctttttcga tacgccagcc agcgaaaatg gctgacaagg
    20941 gaaaccggcc agtaaaacat cgtgttcagg aatgtgttga cgaatatgtt ccgccgccgc
    21001 ctcatcactc acgccttctt tatggctgag ggtgatgtcg cggatatctt cattaaaatg
    21061 atgcgtcgcc gggtcgcaat aatgattggc tttataagtg cgtaccgcat gtttgttcca
    21121 ttcgctggta aacacgcact gtccgccaat cgattcaaaa ccgcgacgga tgccgccaat
    21181 tccggcaaat agatcgataa agcgaaacgc ataatgcgga tgatgtgccg gtggtttggg
    21241 taacaacgtt tgcagatggg cgaactcttt ctcacttaaa cggtgccatg ccgagtcatt
    21301 cgccagcgca cgttttaaaa ttgccgcgct ccagtgattc tcacccacac cattaagctg
    21361 agccaccagc gttttaacat cataaatttg cagcagtttc tccagcattg cctgtgcggc
    21421 attcccggtg ctgtatgaat cggttactga tatattttcc tgcatagatt taaccggcca
    21481 tctgagatgg ccgacaggtt aacataatta cagccattta gggccacgcg cttagcccgc
    21541 gggtgaaaac gcatctatca ccttgtgatc gacaccttca ttctcccccg ccagttcggc
    21601 actgagcttc gccataaact cgactaaaaa ttgcgcgttg tgctgcgcca gctgcttgcc
    21661 tcttacggtt tgcatggttt gcggtagctt caacaactta gtctggaaat gatccagcgc
    21721 gtagcgttta tcatcaagcg ggcgatgctg tgcgagcgga tcttcggcat caaacagcgc
    21781 cacgcccaat gcccctgaaa cggcaaacac gcgcgccagg ccaatcgccc ccagggcttc
    21841 cagccgatcc gcatcctgga cgattttagc ttccgttgtt aagggggcga tttgcgcgct
    21901 gaaactgtga gcggcaatgg catgacaaac ggcctcaatt ttctccgccg gaaattgctc
    21961 gaactcttca cagagcaggc gacgcgtctc ttctgccgcc aggattgaag aacgctgccg
    22021 ctgcggatga tttttcgcca ggctcacaat atcgtgaaaa taacaggcgg ttaaaatcac
    22081 cagcatatcg acatcgtcat ctgccgccag cttctgtgcc gtagcccaga cgcggcgaaa
    22141 atgacacacg tcatgggccg cgtcctggtg ctgatgatgg tttttcaacc agttttcgaa
    22201 ctgtgcctgc cagtgttgta agtccatacg cgctccgttg ccaaagagtc gccaccttag
    22261 cagtttttat tcatagcgtc tgctacgtgc gggacgaggt cgggtgtacc aggcaatgct
    22321 gcctaacaat gccccgacca gagccataaa taaaaatccg accagtgccc ctaaccactt
    22381 tcccgcggta gagcctaaat cgctttcaaa tcccgacacg ggggcatcgg ggatccggct
    22441 catcacccag atgtaacgcg ccattgccca gacgaaatag ccacaccgtg tatagaaagc
    22501 ccacaaagcc agtttgccgc cagggctgcg agagagtctc tcttccatct ttgtcgaacc
    22561 gtattccatt ttgagataac ccggaaaatt cagacttagt actattatgt gataaaagtc
    22621 acatt
//
LOCUS       DAJOWB010000074        22580 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJOWB010000074 DAJOWB010000000
VERSION     DAJOWB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.074"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(218..1129)
                     /gene="gsiD"
                     /locus_tag="OHO85_003042"
     CDS             complement(218..1129)
                     /gene="gsiD"
                     /locus_tag="OHO85_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCQ2672386.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICGVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1132..2052)
                     /gene="gsiC"
                     /locus_tag="OHO85_003043"
     CDS             complement(1132..2052)
                     /gene="gsiC"
                     /locus_tag="OHO85_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCQ2672387.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2070..3608)
                     /gene="gsiB"
                     /locus_tag="OHO85_003044"
     CDS             complement(2070..3608)
                     /gene="gsiB"
                     /locus_tag="OHO85_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCQ2672388.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKVQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3628..5499)
                     /gene="gsiA"
                     /locus_tag="OHO85_003045"
     CDS             complement(3628..5499)
                     /gene="gsiA"
                     /locus_tag="OHO85_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCQ2672389.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSCKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5486..6451)
                     /gene="iaaA"
                     /locus_tag="OHO85_003046"
     CDS             complement(5486..6451)
                     /gene="iaaA"
                     /locus_tag="OHO85_003046"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCQ2672390.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGESPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6655..7890
                     /gene="moeA"
                     /locus_tag="OHO85_003047"
     CDS             6655..7890
                     /gene="moeA"
                     /locus_tag="OHO85_003047"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCQ2672391.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            7890..8639
                     /gene="moeB"
                     /locus_tag="OHO85_003048"
     CDS             7890..8639
                     /gene="moeB"
                     /locus_tag="OHO85_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCQ2672392.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8715..9377)
                     /gene="fsa"
                     /locus_tag="OHO85_003049"
     CDS             complement(8715..9377)
                     /gene="fsa"
                     /locus_tag="OHO85_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCQ2672393.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            9508..10407
                     /locus_tag="OHO85_003050"
     CDS             9508..10407
                     /locus_tag="OHO85_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCQ2672394.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10413..12845
                     /locus_tag="OHO85_003051"
     CDS             10413..12845
                     /locus_tag="OHO85_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCQ2672395.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            12991..13806
                     /gene="ybiV"
                     /locus_tag="OHO85_003052"
     CDS             12991..13806
                     /gene="ybiV"
                     /locus_tag="OHO85_003052"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCQ2672396.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPHFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            13958..15223
                     /locus_tag="OHO85_003053"
     CDS             13958..15223
                     /locus_tag="OHO85_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCQ2672397.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(15464..17056)
                     /locus_tag="OHO85_003054"
     CDS             complement(15464..17056)
                     /locus_tag="OHO85_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCQ2672398.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            17275..18195
                     /gene="ldtB"
                     /locus_tag="OHO85_003055"
     CDS             17275..18195
                     /gene="ldtB"
                     /locus_tag="OHO85_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCQ2672399.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18254..19372)
                     /locus_tag="OHO85_003056"
     CDS             complement(18254..19372)
                     /locus_tag="OHO85_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCQ2672400.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(19369..19836)
                     /gene="mntR"
                     /locus_tag="OHO85_003057"
     CDS             complement(19369..19836)
                     /gene="mntR"
                     /locus_tag="OHO85_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCQ2672401.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20022..20150
                     /gene="mntS"
                     /locus_tag="OHO85_003058"
     CDS             20022..20150
                     /gene="mntS"
                     /locus_tag="OHO85_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCQ2672402.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            20422..22005
                     /gene="opgE"
                     /locus_tag="OHO85_003059"
     CDS             20422..22005
                     /gene="opgE"
                     /locus_tag="OHO85_003059"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCQ2672403.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22054..22569)
                     /gene="ompX"
                     /locus_tag="OHO85_003060"
     CDS             complement(22054..22569)
                     /gene="ompX"
                     /locus_tag="OHO85_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCQ2672404.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
BASE COUNT         5529 a         6203 c         5633 g         5215 t
ORIGIN      
        1 ttatcagcct tatctttatc ttttcgtata aactcagcat gttaatacat ttaacaaaat
       61 aactatctga acgctgtgcc gctgcaaaaa tagtcccttg ctgcggcttt ttaaccaaat
      121 tacccgattc tacaaaatca gcagaaagtt acaacacttg cttagggcaa taattgcctg
      181 tgaggtgatt accctttcaa gcaatattca aacgtaacta tcctttaatt ttcggatcca
      241 gcgcatcgcg taaaccatcg cccaacaaat tgaacgccag tacggtcaga aaaatagcca
      301 gggccggaaa aacagcgaca tgcggcgcga taaccatatc cgctcgtgcc tcattgagca
      361 ttgctcccca ctctggtgtc ggcggctgcg caccgaggcc gagaaatgac aggctggcgg
      421 cagagataat cgaggtgcca atgcgcatgg tgaaaaacac cacgatagac gagacggtcc
      481 caggcaagat atgacgcaat aaaatggtca tatcgctggc accaatactg cgtgctgact
      541 caataaaggt ttgctgtttc aacaccagcg tgttgccgcg caccaggcgg gcaaacgcgg
      601 ggatggaaaa aatggcgact gcgataatca cgttagcaat gccgcttccc aacaccgcaa
      661 caacagcgat cgccagtaaa atacccggga aggcaaacag cacaccgcaa atgcgcatga
      721 tcagccgatc ccaccagcct tcataatatc cagccagcaa gcccagcaac gtccctatcg
      781 ccgcaccgat aaacacggca aacacgcccg ccgccagcga gatttgcgca ccaaccagga
      841 cacggctgaa aatgtcgcgc cccagtgaat cgacgccaaa ccagtgctgc aaagaaggtc
      901 cgttattcag attgtcataa tcaaaataat tttcggcgtc atagggagcg atccagcgtg
      961 caaaaatggc caccacaatc aataaaataa cgaataatgc ggcggtcatc gccatatgct
     1021 gacggcgaaa tcgtcgccag aattcatgcc acggtgtacg cacctggtca ggtttgacca
     1081 gtggcatggc gtttaacacc gcctgacgtc gccagttaaa tagtcgcatc cttacttgta
     1141 cctgatagcc gggttaatgg cggcgtaaag cacatccacc actaagttga taagaataaa
     1201 ttccagcgag aaaagcagaa tttccgcctg aatcaccggg taatcgcgca tttctacgga
     1261 gtcaacgagt aagcgtccaa gtccgggcca gttgaaaact ttctccacca cgatagaacc
     1321 accgagcaaa aagccaaact gtaagcccat catggtcact accgggatca tcgcgttacg
     1381 tagcccgtgt ttgaggacaa cccaggtttc gctcacccct ttcgccctcg cggtacgcat
     1441 ataatcttcg cttaaaacat cgacaaacga cgcgcgggta aagcgcgcca tcacggctgc
     1501 cactgccgcg ccgagcgtca gggagggtaa aatgtagtgc tgccaactgt ctgctcccac
     1561 ggtaggcagc cagcccagtt caacggagaa tacctgaatt aaaagcatcc ccagcgcaaa
     1621 tgccggaaac gagatccccg acaccgcaat ggtcatactc aatcgatccg gccaacgatt
     1681 acgccagacg gcggcgataa ttcccgccgc cataccaaat atcaccgccc agaccatact
     1741 ggttatggtc agccacagcg ttggcataaa gcggctggca atctcatcgg caaccggacg
     1801 acgcgacacc atcgacaggc caaaatcccc ctgcacagca ttgctgatat agtgccagaa
     1861 ctggtgatac agcggctgat ccaaccccag ctgctgacgc accagttcta taacctgcgc
     1921 atcagcttcg ggcccggcaa tcaatcgcgc cggatcgccg ggcagcatat gcacaaataa
     1981 aaacaccagc accgagacga taaacagcgt cggaatcaac cccagtaagc gtttgataac
     2041 gtaattaagc attccactcc ctgcgttgct tattgcaaat ccgcgtcttc aaagctgaag
     2101 ccggtgtctg gcatgatcca aaaaccggtc aggtttttac tgtgtgccga caccagtttt
     2161 tctaccacca gcgggatcca cggcgactct tgccagatga tatcctgcgc cgccttatac
     2221 aagcgggtct tttccgccgg atcattagtt ttcagtgcct gagccaggaa gtcgtccacc
     2281 tgtttattgc tgtaaaatgc ggtattaaac agcgtcggtg gccagttttg cgaggcgaac
     2341 agtggcgata gcgcccagtc cgcttcgccg gttgaagccg accagccagt gtagaacatc
     2401 cgcacgccgc tctctttttg ccctttacct tcaacttctg ccgcccgctg tccggcatcc
     2461 atcgcagtca cctggacttt aatcccgacc tgcgctaact gctgctgggt aaattgcagc
     2521 actttctgcg cggtgctgtg gttatgtgac gaccacagcg tggtactgaa accgttggga
     2581 tatcccgcct ctttcagtaa ttcgcgcgct ttcactggat cgtaaggcca cggtttataa
     2641 ctttgcgcgt aggcgatact tggcggtacc acaccagtag ctggcgttgc atagcccgca
     2701 aaggcaactt tcaccagcgc cggacggtta atggcgtaat tcagcgcctc acggaccttc
     2761 gggttatcga acggcttttg cgtcacgttc atactgatat aacgctgcat aattgacgga
     2821 ctggccatca actcgatatt tttgtttttc tccagcagtg tggcctgctc gtagggaatg
     2881 gggaaagcaa actgcgcttc accggtttgc agcattgccg cgcgggtgtt gttatccgcc
     2941 accggacgcc aggttatgct gtccagtttg ggcaatcctg gctgccagta acccgcgaat
     3001 tttttcacct tcacaaaatc ggtctgattc caggtatcca gttcatacgg tccggttccc
     3061 accggatgaa aaccaatctc cttgccatat ttttccagcg ctgccggtga aatcatcgcg
     3121 gtcgccggat gggcaagaat attaataaac gctgagaacg gctgtttgag ggtaatcttt
     3181 actgttgttg gatcgatcgc ctccgttttg gcaatattct tatacaggtt atagcgttta
     3241 agatgattcg ccggatcgct ggcccggtcc agattcgctt tcaccgccgc ggcgttgaaa
     3301 tcggtgccat cctggaattt aatgccttcc cgcaatttca cggtgtaagt aatgccgtca
     3361 tcggaaacgg tataactctc cgccagcacg tttttcagtt tcatctcttt atccagaccg
     3421 aacagcccct ggtaaaacga tttcgctacg gcctgagata acgtgtcatt cgcgtcatac
     3481 ggatcgagcg tggtgaaatt cgatcctacc gccaccacca catctttggc agcgaatgca
     3541 caagatgcca tcaacgctgt cgcaatgccc agcgccacta acccactacg gtgtacagct
     3601 cttgccattt tattctccgc ctgaatgtta tctacgcatg aatgcgtatt ctgattgtgg
     3661 ttgtgcgacg taatgccccg gcccgacgca ttgcaacgag acggctgcca cctcttcgcc
     3721 acgcagatga atattgctgg gaagatcgtc cgacagcagt acacgctgcg gtcgttgtcg
     3781 ggacggttca gcgaccggaa ctgccgccag taatttacgc gtataaggat gctgcgggtt
     3841 ttcgaagacc gcgcgccgtg gaccaatttc aacaatttgc ccgagataca tcaccgccac
     3901 acgatgacta atccgctcta ccacagccat atcgtgggag ataaacagat acgcaatgcc
     3961 gaaatcacgc tggagatcga gcaacaagtt gataatctgc ccgcgaatag aaacatccag
     4021 cgccgaaacg gcttcgtcgg caatgatcac ttttggattc aatgccaacg cgcgagcaat
     4081 gcagatgcgc tggcgctgac cgccggaaaa ctcatgcggg taacgccagg catgttcagg
     4141 taacaggccc acgcgctcca gcaaccacgc aacgcgtgca gccgcttctt tacctggcaa
     4201 taaaccgtgt acacgcagcg gttcgataat cgaatcaccg atggtctgac gtgggtccag
     4261 cgaagcgtaa gggtcctgaa aaataaactg aatatcccgg cgcaatgcct gaagtttgcc
     4321 gggtgacaag gtatcgattc gctgaccgtt aaagataatt tcgccgccct gcgattcgac
     4381 caggcgcagc aacgcccgcc cggtagtgga tttaccgctg ccagactcgc ccaccagcga
     4441 tagcgtttcg ccaggccaga gatcaaaact gactttctca acggcatgca cttcccgcgt
     4501 tacgcgattc aacaaaccgc tgcgcaaagg gaaacgggtg accagattac gcacccgtaa
     4561 aacaggttca ccatccacca ccgttttctg ctcgatgggg ggggcctgtt tcgctggatg
     4621 ttcaagcgat atcaatggga aacgtcgggg ataatctaac cctttcatcg caccaagttg
     4681 cggaacagca gctaacagcg cacgggtgta aggatgttgc ggtgcatgaa aaatctgttc
     4741 gacggtaccc gtttccaccg cctcgccctg atacatcacc agtacccgat cggcaatctc
     4801 tgccaccacg cccatatcgt gagtgataaa gataacgccc atcgacatct ctttttgcaa
     4861 tactttgatt aattgcagga tctgcgcctg aatagtgaca tccagcgcgg tggttggctc
     4921 atcggcaatc agcaccgccg ggcggcatga cagcgccatc gcaatcatca ctcgctggcg
     4981 catcccgcca gagagttgat gcggataacg tgaaagaatg gtttgtgcct caggaatgcg
     5041 tacctgatcc agcatccgct tcgcctcgac catcgcttct tcacgactgg cgttctgatg
     5101 cagacgaatt gattcggcaa tctgttcacc cacagtaaat accgggttca gcgatgtcat
     5161 cggctcctga aatatcatcg ccatatccgc accgcgaaca tggcgcattt gtgcagcgct
     5221 ctgctcgcta agttcaatca cttcgcgact gcgccgctgc aacagcattt tatcgcactg
     5281 tactaaaccg cccgcctgtt ccaacaggcg catcaatgcc aacgcagtca ctgacttaca
     5341 ggagccggat tcgccaacaa ttgccagcgt ctcaccgcgt tgcagactaa aagagagatt
     5401 gcggaccgca gctattttct gctggtcctg cataaaggca atattcagat tttcaaccgc
     5461 cagcacatta ccggcatcaa gttcatcact gtgtggcaac ggtgtcccct ttttcacggt
     5521 agataccggt ggttggcgta tcgcctgcgt agccccaggc gcgatacatt ccttcggtgt
     5581 taaacggtag cgcgacattc ccttcatggt cgatagcgat taagccaccg ctaccgccaa
     5641 gcgcagggag tttttccatt actacccgct cgcaggcttc cgcgagactt aatccgccgt
     5701 aatccattaa cgcggcgatg tcatatgccg ccagcgcgcg gatgaagact tcgcccgtgc
     5761 cggtacaaga aaccgccaca ctggcgttat tggcgtagca tccggcaccc actaagggac
     5821 tttcgccaac tcgtccgggt aatttattgg tcattccgcc tgtggacgtg gctgccgcca
     5881 aattgccgtc taaatccaac gccacggccc ccacggtgcc cattttctgt ttttcatcca
     5941 gtggcgcacc gctatggtcg aggacggttg ccccttcctt gcgcgctgcc agtagttgtt
     6001 cataacgcaa aggcgtggag aaaatctccg gcgagacgcg ctccatgcca cgagcaaacg
     6061 caaaattttc tgccccttcg ccaatcatca tcacatgcgg gctttgctcc atcaccagcc
     6121 gggcggcaag aaccggatta cgcagatgac taacgcccgc caccgcaccg gctttcaggg
     6181 tgttaccatc catcacacag gcgtccagtt catgggtttc atcacgcgta aagacagcgc
     6241 caattccggc gttaaacagt ggacactctt ccagcagacg caccgcttcc gtcaccacat
     6301 ccagcgcact ttcgcccgct tccagcattt tctgcccggt ttcaacaatg gcagacaacg
     6361 cctcaatgta gcgtaattcc tgttgcagac tcatctgcgc gcggctaatt gcacctgcgc
     6421 caccatgaat tgcaatgact gctttgccca tagtattcgt ccattaaggg tggctcgcca
     6481 cttttgctat aaatatcaga atttttatga attactcatt caatttttga atatagaaag
     6541 atggatatgg catgtaaagg caggagccaa cgctacgccc gaggtggcat acagtctgtg
     6601 gcgtttttct gacataatag gcaaattcga ttttgcctcc gcaggagtgt tttcatggaa
     6661 tttaccaccg gattgatgtc gctcgacacc gcgcttaatg agatgctttc tcgcgtcacc
     6721 ccactgaccg cccaggaaac gctgccactg gtacagtgtt ttggtcgtat tctggcgagc
     6781 gatgtcgttt cgccgcttga tgttccgggg tttgataact ccgcaatgga cggctacgcg
     6841 gtgcgtttag ccgatattgc ctccaggcaa ccgctgcccg ttgccggtaa atcctttgcc
     6901 ggtcagccgt accatggtga atggcctgcg ggtacctgca ttcgtattat gaccggtgcg
     6961 ccggtgccgg aaggctgcga agcggtggtg atgcaggagc agactgaaca aacggacaat
     7021 ggcgtgcgtt ttactgctga agtgcgtagc gggcaaaata ttcgccgtcg cggtgaagat
     7081 atctctgcag gtgcggttgt tttcccggcg ggaactcgcc tgactaccgc tgaactgcca
     7141 gtgattgctt cactggggat tgccgaagtt ccggtgattc gtaaagtgcg tgtagcgctt
     7201 ttttctaccg gtgatgaact ccagttgccc ggtcagccac tgggcgatgg ccaaatctac
     7261 gataccaacc gtctcgccgt acacctgatg ttagaacagt tgggatgcga ggtaatcaac
     7321 ttagggatta tccgcgacga tccacatgcc ctgcgcgccg catttattga agccgacagc
     7381 caggcggatg tggtgatcag ttccggcggt gtttcagtgg gtgaagcgga ttacaccaaa
     7441 acgattcttg aagagctggg ggagatcgcc ttctggaagc tggcgattaa accaggtaaa
     7501 ccgttcgcgt tcggtaaact cagcaatagc tggttctgcg gcctgccggg caacccggtt
     7561 tcagcgacgc tgaccttcta tcaactggta cagcctttgc tggcaaaact aagcggcaac
     7621 accgccagcg gcctgcccgc gcgccagcgc gtacgcacag cgtcccgcct gaaaaaaacg
     7681 ccaggacgtc ttgatttcca gcgcggcgtg ctgcaacgca acgccgacgg tgaacttgaa
     7741 gtgacgacca ccggacatca gggttcacat atatttagct cctttagtct cggcaactgc
     7801 tttatcgtgc tggaacgcga tcgcggcaat gtggacgtgg gcgaatgggt ggaagtagaa
     7861 ccgtttaacg cgttgttcgg aggcctgtaa tggcggaact cagcgatcag gagatgctgc
     7921 gctacaaccg gcaaatcatt ctgcgcggtt ttgattttga cggtcaggag gcgctaaaag
     7981 attctcgtgt gctggtagtc ggcctcggtg gactcggctg cgcagcttcg cagtatctgg
     8041 caagcgccgg tgtcggcaac ctgacgctgc tcgacttcga cacggtttcg ctctcgaatc
     8101 tgcaacgcca gacgctgcac agtgatgcta cggtcgggca accgaaggta gaatccgccc
     8161 gtgacgccat gacgcggatc aacccacata tcgcgattac gccagtcaat gcactgcttg
     8221 atgacgctga acttgctgcg atgattgcaa aacacgatct ggtactcgac tgtacggata
     8281 acgttgcggt gcgtaatcaa ctgaacgctg gctgttttgc cgcgaaggtg ccgctggttt
     8341 ccggcgcggc aattcgtatg gaagggcaaa tcacggtctt cacttatcag gacggcgaac
     8401 cgtgctatcg ctgccttagc cgtttgtttg gcgagaacgc attaacctgc gtagaagcag
     8461 gcgtgatggc accgttgatc ggcgtaattg gttcgttgca ggcaatggaa gcgatcaaac
     8521 tgctggcagg ttatggcaaa ccggccagcg ggaaaatcgt catgtacgat gcgatgacct
     8581 gtcagtttcg cgaaatgaaa ctgatgcgca atccggggtg tgaggtgtgc gggcagtaat
     8641 tgccacctcc aggccagata agacgcggca ctccgccacg tcatcagcaa tttatgagga
     8701 tgcagaacgt ccggttaaat cgacgttctg ccaaacgctc ccagccagtc ctgttcaaac
     8761 ttcgccacag cggcttccac cgccggatag ctaatcatct gttgtgccac atccagtggc
     8821 agcgtaattg attcacatcc tgccagtaag cagtccagcg cctgacgcgg ggttttgaaa
     8881 ctcgctgcca gcactttcgc ctgcggcgca tgcattttca ataactggtg taagtcggtc
     8941 acagtctgaa tgccgctacc gccctgagca tcaatacgat taacgtaagg cgcaacatat
     9001 tccgcacctg ccagcgccga cagcagccct tgtgctgcgc catataccgc ggttcccagc
     9061 gttggaatcc cttccgcttt taacatctta atagctgcca gcccctcggc ggtcaccgga
     9121 actttcacca cgatatccgc aataatagaa cgcaatttac gtgcgtcatt caccatccct
     9181 tcagcagtgg tagccattac ctgggcaaac agacgcccct gaccgcccat cgcttcatga
     9241 agttgcggaa gcacaacatc cagcggtttt ttacccgcgg cgataatgct tgggttagtg
     9301 gtcacacccg ccagcggaaa aatacgtgac agcgccttca ccgcaacaac gtctgaagta
     9361 tccagataca gttccataac catcctcaaa atgtactttt tgtctgaatg tagactatca
     9421 cgggaaaact ctctgaacag ttgacgcaca tcaatctaac tttcattcga aagtatttta
     9481 atgtttgaac gaaacagaga ggcaactatg attttcaata ttcagcgcta ctcgacccat
     9541 gatggccccg gtatccgcac ggtcgtattt cttaaaggct gttcgctggg ctgccgctgg
     9601 tgtcagaacc cggaaagccg cgcccgcacg caggatctgc tgtatgacgc acgactgtgt
     9661 ctggaaggct gcgagctgtg cgctaaggcc gcgccggaag tgattgagcg cgcgctgaat
     9721 ggtttgctta ttcatcggga aaagttaacc ccggagcatc tgacggcgtt aaccgactgc
     9781 tgtccgacac aggcattaac cgtgtgtggt gaagtgaaaa gcgttgagga gatcatgacg
     9841 accgttctgc gcgataaacc gttttacgat cgcagcggcg gcggtttaac gctttcgggt
     9901 ggtgagccct ttatgcagcc ggaaatggcg atggcgctac tgcaagccag ccacgaggca
     9961 ggcattcata ctgcggtaga aacctgtctg catgtgccgt ggaaatatat cgccccttct
    10021 ctgccctata tcgatctgtt tcttgccgat ttaaaacacg ttgccgacgc gccgtttaaa
    10081 cagtggaccg acggtaacgc cgccagagtg ctggataacc tgaaaaaact cgccgcagcg
    10141 ggcaaaaaaa tcattatccg cgtgccgctg attcagggct ttaatgccga cgaaacctct
    10201 gtaaaagcca ttaccgattt tgccgccgac gagctgcacg ttggcgaaat tcattttctg
    10261 ccctaccaca cgctgggcat caacaaatat cacttactta atctgcccta tgacgccccg
    10321 gaaaaaccgc ttgatgcgcc agaactgctc gactttgccc agcagtatgc ctgccagaaa
    10381 gggttaaccg cgaccttacg aggataacaa ccatgaccac actgaaactg gacacgctca
    10441 gcgaccgcat taaagcgcac aaaaatgcgc tggtgcatat tgtgaaaccg ccagtctgta
    10501 ccgagcgcgc gcagcactat accgagatgt atcaacaaca tctcgataag ccgatcccgg
    10561 tacgtcgcgc gctggcactg gcgcatcacc tggcgaatcg caccatctgg atcaaacacg
    10621 atgagttgat cattggcaac caggcaagcg aagttcgcgc cgcgccgatc ttcccggaat
    10681 atactgtctc gtggatcgaa aaagagattg atgatctggc agatcgtccc ggtgctggct
    10741 ttgcggtgag cgaagagaac aaacgcgttc tgcatgaagt gtgcccgtgg tggcgcggtc
    10801 agaccgtaca ggatcgctgc tacggcatgt ttaccgatga gcaaaaaggt ctgctggcga
    10861 ccggaatcat taaagcggaa ggcaatatga cctccggcga tgcgcacctg gcggtgaatt
    10921 tcccgctgct gctggaaaaa ggtcttgatg gtctgcgtga gaaagtagcg gaacgtcgct
    10981 cgcgcatcaa tctgacggtg ctggaagatt tacatggcga acaattcctg aaagcgattg
    11041 atatcgtgct ggtggcagtc agtgaacaca ttgaacgttt cgctgccctg gcgcgtgaaa
    11101 tggctgctac cgaaactcgc gaaagccgtc gcgatgaact gctgacgatg gcagaaaact
    11161 gcgatcttat cgcccaccag ccgccgcaga ctttctggca ggcgctgcaa ctgtgttact
    11221 tcatccaatt gattttgcag atcgaatcta acggtcactc ggtatcgttt ggtcgtatgg
    11281 accagtatct ctacccgtac tatcgccgcg acgttgaact caaccagacg ctggatcgcg
    11341 aacacgccat cgagatgctg catagctgct ggctgaaact gctggaagtg aacaagatcc
    11401 gctccggctc acactcaaaa gcctctgcgg gaagtccgct gtatcagaac gttaccattg
    11461 gcggacaaaa tctggttgat ggtcaaccaa tggatgcagt gaatcccctc tcttacgcga
    11521 tcctcgaatc ctgcggtcgc ctgcgttcca ctcagcctaa cctcagcgtg cgttaccatg
    11581 caggaatgag taatgatttc ctcgacgcct gcgtacaggt gatccgttgc ggcttcggga
    11641 tgccggcgtt caacaacgac gaaatcgtga tcccggaatt tattaaactc ggtattgaac
    11701 cgcaggacgc ttacgactac gcagcgattg gttgtatcga aaccgccgtc ggtggcaaat
    11761 ggggctatcg ctgtaccggc atgagcttta tcaacttcgc ccgcgtgatg ctggcggcgc
    11821 tggaaggcgg gcgtgatgcc accagcggca aagtgttcct gccacaagaa aaagcgttgt
    11881 cggcaggcaa ctttaacaat ttcgatgaag taatggacgc gtgggatacg caaatccgtt
    11941 attacacgcg taaatcaatc gaaatcgaat atgtcgtcga caccatgctg gaagagaacg
    12001 tacacgatat tctctgctcg gcgctggtgg atgactgtat tgagcgagcg aaaagtatca
    12061 agcaaggcgg cgcgaaatat gactgggttt ctggcctgca ggttggcatt gccaacctcg
    12121 gcaacagcct ggcggcagtg aagaaactgg tatttgaaca gggcgcgatt ggtcagcaac
    12181 agctggctgc cgcactggcg gatgacttcg atggcctgac tcatgagcaa ctgcgtcagc
    12241 ggctgattaa cggtgcgccg aagtacggca acgacgatga taccgtcgat acgctgctgg
    12301 ctcgcgctta tcagacctat atcgacgaac tgaaacagta ccataacccg cgctacggtc
    12361 gtggtccggt tggcggcaac tattacgcgg gtacctcgtc tatctccgct aacgtaccgt
    12421 ttggcgcgca aactatggca acaccggacg ggcgtaaagc ccacaccccg ctggcagaag
    12481 gcgctagtcc ggcctctggt actgaccatc tcggccctac tgcggtcatt ggctcggtgg
    12541 gtaaactgcc tacggcagcg attctcggcg gcgtgttgct caaccagaaa ctgaatccgg
    12601 caacgctgga gaacgaatct gacaagcaga aactgatgat cctgctgcgc accttctttg
    12661 aagtgcataa aggctggcat attcagtaca acatcgtttc ccgcgaaacg ctgctggagg
    12721 cgaaaaaaca tccggatcag taccgcgatc tggtagtgcg tgtcgcgggc tattccgcct
    12781 tcttcaccgc gctctctcca gacgcacagg acgatatcat cgcccgtact gaacatatgc
    12841 tgtaatctcc tcggcccggc ggcgtcgtcg ccgggctaaa tcattcacat catcaatttc
    12901 atccttactt tcattcgaaa tataatttgt gctctgcgtc acattgttat tacactgtta
    12961 cggtttcatt ttcgagccag gagcacacct atgagcgtaa aagttatcgt cacagacatg
    13021 gacggtactt ttcttaacga cgccaaaacg tacaaccaac cacattttat ggcgcaatat
    13081 caggaactga aaaagcgcgg cattaagttc gttgtcgcca gcggtaatca gtattaccag
    13141 cttatttcgt tctttcctga gctaaaggat gagatctctt ttgtcgcgga aaacggcgca
    13201 ctggtttacg aacatggcaa gcaactgttc cacggcgaac tgacccgaca tgaatcgcgg
    13261 attgttattg gcgagttgct aaaagataag caactcaatt ttgtcgcctg cggtctgcaa
    13321 agtgcatatg tcagcgaaaa tgcccccgaa gcatttgtcg cactgatggc aaaacactac
    13381 catcgcctga aagctgtaaa agattatcag gagattgacg acgtactgtt caagttttcg
    13441 ctcaacctgc cagacgaaca aatcccgtta gtgatcgaca aactgcacat agcgctcgat
    13501 ggcattatga aacccgtcac cagtggtttt ggctttatcg acctgattat tcccggtcta
    13561 cataaagcaa acggtatttc gcggttactg aaacgctggg atctgtcacc gcaaaatgtg
    13621 gtagcgattg gcgacagcgg taacgatgcg gagatgctga aaatggcgcg ttattccttt
    13681 gcgatgggca atgctgcgga aaacattaaa caaatcgccc gttacgctac cgatgataat
    13741 aatcatgaag gcgcgctgaa tgtgattcag gcggtgctgg ataacacatc cccttttaac
    13801 agctgacctc tctaccggag ccgtgcgctc cggttttccc ttctttttac atcctgtaca
    13861 tcacatcaaa tcattaaact tgcattaact tatttataac ttatattgtc acatgcaaat
    13921 cagaatggtt tcgaatgaaa gttaatgaga ggtggttatg gcttctactt ttaccagcga
    13981 cacattgcct gccgatcaca aagcagctat ccgtcagatg aagcacgcgc tgcgggcgca
    14041 gcttggcgac gtccagcaga tctttaatca gctaagcgat gacattgcca cgcgagtggc
    14101 tgaaatcaac gcactcaaag cacagggcga tgccgtctgg ccggtgctgt cttatgccga
    14161 tatcaaagca ggtcatgtta ctgcagagca gcgcgaacag attaaacgtc gcggttgtgc
    14221 ggtgataaaa ggccatttcc cccgcgaaca agcgctaggc tgggatcagt cgatgctgga
    14281 ctatctggac cgcaaccgct ttgacgaggt ctacaaaggc cccggcgata atttcttcgg
    14341 gacgctcagc gcttcacgtc ccgagattta ccccatctac tggtcgcagg cgcaaatgca
    14401 ggcccgccag agtgaagaaa tggcgaatgc gcagtcgttt ctcaatcgtc tgtggacatt
    14461 tgaaagtgat ggaaagcaat ggtttaaccc ggatgtgagc gtcatctacc ctgaccgtat
    14521 ccgccgccgt ccgcccggaa cgacctccaa aggtcttgga gcgcataccg actccggggc
    14581 gctggaacgc tggctgcttc cagcgtatca gcgcgttttc gccaacgtct ttaatggcaa
    14641 tctggcgcaa tatgatccct ggcatgcggc acatcgtacg gaagttgaag agtacacggt
    14701 ggacaacacc accaaatgtt ccgtgtttcg gacattccag ggctggacag cgctctctga
    14761 tatgctgcct ggtcaggggc tgctgcacgt tgtgcccatt cctgaagcca tggcgtacgt
    14821 actgttacgt ccgctgcttg atgatgtgcc ggaggatgaa ctgtgcggcg tagcgcccgg
    14881 aagagtgttg ccggtatcag agcaatggca tccactgtta attgaggcgt taaccagcat
    14941 tccaaaactc gaggccggag actccgtctg gtggcactgc gacgtcatcc attccgttgc
    15001 ccccgttgaa aatcaacagg gttggggcaa cgtgatgtac attcctgcgg caccgatgtg
    15061 cgagaaaaat cttgcctacg cgcacaaggt gaaggccgca ctggaaaaag gcgcatcgcc
    15121 gggcgacttc ccgcgcgagg actatgaaac aaactgggaa ggacgcttta cgcttgccga
    15181 cctcaacatt cacggtaagc gagcgttggg catggatgtt tgagagttaa tggaagtgaa
    15241 ttgatgtggg attaagaaaa tcgattcgac gttactcaga ttgctgacaa gtgcgctttc
    15301 ttaatgcctg atgcggcgtt aacgccttat ctggcctaca aagtcctgca gattcaatat
    15361 attacaggta ctgcgtaggc ctgataagcg tagcgcatca ggctattttg catttgtcat
    15421 cagccgccgt caccccgaca gtatcggggt gacggataat cacttactcg atccctttac
    15481 tacgcaggta atcttcgtaa ttaccgctaa agtcgatcac gcgttccggg gtgatttcca
    15541 gaatgcgggt cgccagggag cttacgaact cacggtcgtg tgaaacaaag atcagcgtgc
    15601 cctgatacag ttccagcgcc atgttcagtg actcgatgga ttccatatcc aggtggttgg
    15661 tcggttcgtc catgatcaga atgttcggct tctgcatcat taacttacca aacagcatcc
    15721 gccctttttc cccaccggaa agcactttag ctggcttttt gatgtcgtcc tggctgaaca
    15781 gcaaacgacc gagaatgctg cgtaccgcct gctcgtcatc gccttcctgc ttccactgac
    15841 tcatccattc gaacacggtc agatcatttt caaactcata ttcgtggtcc tgggcgtagt
    15901 aaccaatgcg cgcgttttca gaccatttta cggtgccgct gtccggttgc agatcgccca
    15961 ccagcgtttt cagcagcgtt gatttaccga cgccgttggt acccagtacc gcaagttttt
    16021 caccgacctc cagcagcagg ttgagatttt taaacagtgg accgttatca aaccctttgg
    16081 tcagaccttc cacttccagc gcgttacgga acagtttctt atcctgttca aaacggatga
    16141 acgggttctg acggctggag gctttcacct cttccagttt gattttatca atctggcgcg
    16201 cgcgcgaagt tgcctggcga gatttcgagg cgttggcgct aaagcggcta acgaaagatt
    16261 gcaactcagc aatctgcgct ttcttcttgg cgttatcggc cagcagacgt tcacgcgcct
    16321 gggtcgccgc cgtcatgtac tcatcgtagt tacccggata aacgcgcagc tcgccgtaat
    16381 ccagatccgc catgtgcgta cagaccatgt taaggaagtg acggtcgtgc gagatgatga
    16441 tcatggtgct gtcacgctcg ttcagcacct gttccagcca gcgaatggtg tcgatgtcga
    16501 ggttgttggt cggttcgtcg agcaggagaa tatccggatc agcaaacagc gcctgcgcca
    16561 gaagcacacg cagcttccag ccaggagcaa cttcactcat cgggccgtag tgctgttcca
    16621 ctggaattcc cacgccaagc aacaattcac cggcgcgagc ttccgcagag taaccgtcca
    16681 tttcgccgta tttaacttcc agatcggcca ctttatagcc gtcttcctca ctcatttccg
    16741 gcaaagcata gatgcggtca cgctcctgct tcacttccca caactcttta tgccccatga
    16801 tcaccgtatc cagcacagtg aactcttcaa aggcaaactg atcctgacgc agcttaccaa
    16861 tgcgctcgtt gggatcgagg gaaacgttac ccaaagtcgg ctctaagtcg ccgccgagga
    16921 tcttcataaa ggtggattta ccactaccgt tcgcgccaat caggccgtaa cggttgccgc
    16981 cgccaaattt gacggaaatg ttttcaaaca acggcttact gccgaactgc atggtgacgt
    17041 tactggaaac taacacaggc gtatcctgaa aagagatatg acaaaccgcg cattatgcca
    17101 taagtctgaa agaagatcct gctcctcgct taaggtctac actcaagcca aaggcaaaaa
    17161 atgtgatttc gtacacatct gatttcactg tgagctggaa tgaacttata atgcgcttcc
    17221 aatactctaa tattctcaat ccaatggcct gccaggcaca aaatctcgct taacatgaat
    17281 atgaaattga aaacattatt cgcagcggcc ttcgctgttg tcggcttttg cagtaccgcc
    17341 tctgcggtaa cttatcctct gccaaccgac gggagtcgcc tggttggtca gaatcaggtg
    17401 atcaccattc ctgaaggtaa cactcagccg ctggagtatt ttgccgcgga gtaccagatg
    17461 gggctttcca atatgatgga agcgaacccg ggtgtggata ccttcctgcc gaaaggcggt
    17521 actgtactga acattccgca gcagctgatc ctgccggata ccgttcatga aggcatcgtc
    17581 attaacagtg cagagatgcg tctgtattac tatccgaaag ggaccaacac cgttatcgtg
    17641 ctgccgatcg gcattggtca gttaggcaaa gatacgccta tcaactggac caccaaagtt
    17701 gagcgtaaga aagcaggccc gacctggacg ccgaccgcca aaatgcacgc agagtaccgc
    17761 gctgcgggcg aaccgcttcc ggctgtcgtt ccggcaggtc cggataaccc gatggggctg
    17821 tatgcactct acatcggtcg cctgtacgct atccatggca ccaacgccaa cttcggtatc
    17881 ggcctgcgtg taagtcatgg ttgtgtgcgt ctgcgtaacg aagacatcaa attcctgttc
    17941 gagaaagtac cggtcggtac ccgcgtacag tttattgatg agccggtaaa agcgaccacc
    18001 gagccagacg gcagccgtta tattgaagtc cataacccgc tgtctaccac cgaagcccag
    18061 tttgaaggtc aggaaattgt gccaattacc ctgacgaaga gcgtgcaaac agtgaccggt
    18121 cagccagatg ttgaccaggt tgttcttgat gaagcgatta aaaaccgctc cgggatgccg
    18181 gttcgtctga attaatcttc aaaacttaaa gcaaaaggcg gactcataat ccgccttttt
    18241 tatttgccag accctagttg gccgggagta taactaacaa aacatatccc accaacgccg
    18301 cccacaacag catcggtatt gaatagagat ggaaacgcca ccagatgcgg cgatcgttcg
    18361 ccatacgtag cgcaatcaaa tttgccagcg atccgggtaa taacccaaag ccacctacgt
    18421 ttaccgccca taccagtaat aaagacggcg gcacatagtt cagcaacaat atggtactcg
    18481 gcacgttact gatcacctgc gataaaccga ttgccgttaa ccataacccg ggttcagata
    18541 gatgactcac gttacccaac acgccttgca acgctggaag ctgggtcagt aaatggacgt
    18601 cgataaacat cgccataaac accagcagca gcgtccagtc cacactgagc accacgcgac
    18661 gtgccagcag cgcaaagcct gccgccacaa tcaccagtcc ccacagctct tgtttgaact
    18721 ccagcgccgt cagaaagacg atatacagcc ccagacaact ccacaccagc cgcggtttcc
    18781 actccggtgt ttgcaccccc gtatggtatt gcagcgcctt tccagggaaa caacaccagc
    18841 acaggagcag gagcgtcagc atcattgcgc cagccagcgg tgccatttgg gcaataaatc
    18901 cggcaaacga aagaccagaa cgtccccaga taagaatatt ttgcgggttg ccaattggcg
    18961 tcagtagcga accagcgttg actgccagcg cctcaaaaat aatcagccga ttaaccggga
    19021 tctcacacag tctttttagc gtgatagtca gcggaacaac aataaacagc gcgacatcgt
    19081 tggtcagaaa ggtagaaagc agcgccgccg ccagcaccat aaacatcgcc agccgacgct
    19141 ccgtagcaaa gcggcgcacc attttgcgcc ccagcacatc aaaataaccg cttaactcca
    19201 cacctttggt cagcagcatc aggccgctta aggtgatgat ggtgtgccag tcgatagcag
    19261 caggccagga tttcggtgca aagggcacga aaaagcttaa tccgatacca acaagaatta
    19321 ataactgaaa aaaacgatcg ccttgcagcg tgcgtaaaaa aggcaggctc atttggcacc
    19381 gtgtttctgg gtaaacaaac gaaaagcgtc gagcgtctct tcactaacat ggtgctccat
    19441 gccttccgcg tcgcgacggg cgatttccgg actgacgccc aacaccaaca agaaattttc
    19501 gactatctga tgtcgctcgc ggctttcctg cgccagcttc tctccttctg ccgttaaaaa
    19561 cacgccacgc caggggatca tttcaatcag ccccatggtt gccagccgct taagcatttt
    19621 agccaccgtc ggttgcgaaa ctcccagacg agcagccatg tccacctgac gagcttcccc
    19681 cacttccctg atcaagtcag aaatcagctc aacgtaatca tcaataagct cgcgccgatg
    19741 cgcctctctg acctggcgga acccttcaac gtgctcttcc acgttcacta actgcgtcac
    19801 tttttttgct gttggcgtac ctgcgcgacg actcattgtg cttcctcatt atggtgacgc
    19861 atacatagcg ttctcatttc gtgcgcacat tgtaaaccag agttgcgaag gtacaaaaaa
    19921 ttaacgtttt agcaatagct atataatata gcctgtgcta tatctgtatg taatgcaatc
    19981 atccctcaag gatcgacggg attagcaagt caggaggtct tatgaatgag ttcaagaggt
    20041 gtatgcgcgt gtttagtcat tctcccttta aagtacggtt aatgctgctc tctatgttgt
    20101 gcgatatggt caacaacaaa ccgcagcaag ataaaccttc cgataaatag cggcgtcgcg
    20161 gtacgccgct tcactcctgc tttcatgcag gcataacgcg ttttggtctg aaaaacccca
    20221 ctttttgtcg gatttgcaat ccccttcgca aaagatttgt tcgtcagtag ttgacctgaa
    20281 cggcggcccg ctctatcttc ttgcagccct gcgtatattg cggctcgcgg atgcggaccc
    20341 ctttccactc ttcacgcact cttgcaggta ttgacccttg acgccagggt aagcacatgg
    20401 cgtttgttac gatagtggca tatgaattta accctcaaag aatcgcttgt tacccgtagc
    20461 cgggtattta gcccgtggac tgcgttctac tttttacagt cgctattaat taacctcggc
    20521 ttaggttacc ccttcagttt gctctacacc gctgcgttta cggctatttt gcttttgcta
    20581 tggcgaacat tgcctcgcgt acaaaaagtt ctggtcggtg tcagttcgct ggtggcggct
    20641 tgttatttcc cttttgctca ggcctacggc gcgcctaatt tcaatacatt gctggcattg
    20701 cactccacca atatggaaga gtcgaccgaa atcctgacga tttttccgtg gtacagctac
    20761 ctggtcggct tatttatttt tgcgctcggc gtaatagcaa tcaggcgaaa aaaagagaat
    20821 gaaaaagcgc gctggaatac cttcgacagc ctgtgtctgg tattcagtgt ggcgacattt
    20881 tttgttgctc ccgtgcaaaa cctggcctgg ggtggcgtat ttaaactgaa agatactggc
    20941 tatccggtat ttcgttttgc taaggatgtc atcgtcaata ataacgaggt gattgaagag
    21001 caagaacgga tggcaaaact ttccggaatg aaagatacct ggacggtcac tgccgttaag
    21061 ccgaagtatc agacctatgt ggtggtgatc ggtgaaagcg cgcgtcgcga tgccctcggt
    21121 gcctttggcg gtcactggga caataccccg tttgccagca gcgttaacgg tttgatattt
    21181 gctgactaca ttgccgccag tggctccacg cagaaatcgc ttggcttaac gctcaatcgc
    21241 gtagtcgatg gcaaaccaca gtttcaggat aactttgtca ccctggcaaa tcgcgcgggc
    21301 ttccagacct ggtggttttc caaccagggc caaatcggcg aatacgatac tgctatcgcc
    21361 agcatcgcca aacgtgcaga tgaagtgtac ttcctgaaag aaggtaattt tgaagcagat
    21421 aaaaacacga aagacgaagc gttactggat atgaccgctc aagtgctggc gcaagagcac
    21481 tcgcaaccgc agctgattgt tctgcatctg atgggctcgc atccgcaggc ctgcgacagg
    21541 acacaaggca aatacgaaac ctttgtgcaa tcgaaagaaa cgtcgtgcta tctctatacc
    21601 atgacgcaaa cggacgattt actgcgcaag ctgtacgatc agttacgcaa cagcggcagc
    21661 agcttctctc tggtttactt ttctgaccac ggtctggcct ttaaagagcg cggcaaagac
    21721 gtgcaatacc ttgcccatga tgataaatat cagcaaaatt tccaggtgcc ttttatggtc
    21781 atttccagcg acgataaagc gcatcgggtg attaaagccc gccgctcagc caatgacttc
    21841 ttaggctttt tctcgcagtg gacgggaatt aaagcgaagg aaataaatat caaatacccg
    21901 tttatatctg agaagaaagc cgggccgata tacatcacca acttccagtt acagaaggtg
    21961 gattacaacc atctcggaac cgatattttc gacccgaaac cttaaaaaca aaaatccgcc
    22021 ccgagaggcg gattttttat atcaccaaag tgattagaag cggtaaccaa caccggcaat
    22081 ccaggtgcct acgtcaacgc tacgaatacg gctctgctcg taagagaagt ccagagcaac
    22141 gttttccatc gggttgaact gcagaccagc accgtaggag aaaccgtagt cgctggtgtc
    22201 gtgtttgtag gtcgggtatt cagtggtctg gaatttacca taacccacac ccactacacc
    22261 gtagatgctt gcccagtcgt taatgcggta agccggacca gcagtgatgc cgtagtactg
    22321 gtttttgttg tagtcaccag agcttgcagt acggcttttc tcggtgtaag tgaaagaacc
    22381 gatcacaccc agcgggctgt tgtcttcttc atagcggtat ttcaggttga aaccgcccat
    22441 tttgttcatt tggccctgag cgtcgctctg tgcgtaaccg ccagttacgg tagaagtcgc
    22501 agctacggaa gtacctgcgg tgaaagccag aactgcggcc agtgctgaaa gacatgcaat
    22561 ttttttcata accacctcaa
//
LOCUS       DAJOWB010000075        22524 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJOWB010000075 DAJOWB010000000
VERSION     DAJOWB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.075"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            297..1196
                     /gene="yegS"
                     /locus_tag="OHO85_003061"
     CDS             297..1196
                     /gene="yegS"
                     /locus_tag="OHO85_003061"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCQ2672405.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(1278..2051)
                     /locus_tag="OHO85_003062"
     CDS             complement(1278..2051)
                     /locus_tag="OHO85_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCQ2672406.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(2157..3197)
                     /gene="gatD"
                     /locus_tag="OHO85_003063"
     CDS             complement(2157..3197)
                     /gene="gatD"
                     /locus_tag="OHO85_003063"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCQ2672407.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVDLHAFHLAKGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(3245..4600)
                     /locus_tag="OHO85_003064"
     CDS             complement(3245..4600)
                     /locus_tag="OHO85_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol permease IIC component"
                     /protein_id="HCQ2672408.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(4604..4888)
                     /gene="gatB"
                     /locus_tag="OHO85_003065"
     CDS             complement(4604..4888)
                     /gene="gatB"
                     /locus_tag="OHO85_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCQ2672409.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(4919..5371)
                     /gene="gatA"
                     /locus_tag="OHO85_003066"
     CDS             complement(4919..5371)
                     /gene="gatA"
                     /locus_tag="OHO85_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCQ2672410.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVYDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(5381..6643)
                     /gene="gatZ"
                     /locus_tag="OHO85_003067"
     CDS             complement(5381..6643)
                     /gene="gatZ"
                     /locus_tag="OHO85_003067"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCQ2672411.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIESTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(6672..7526)
                     /gene="gatY"
                     /locus_tag="OHO85_003068"
     CDS             complement(6672..7526)
                     /gene="gatY"
                     /locus_tag="OHO85_003068"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCQ2672412.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(7836..8888)
                     /gene="fbaB"
                     /locus_tag="OHO85_003069"
     CDS             complement(7836..8888)
                     /gene="fbaB"
                     /locus_tag="OHO85_003069"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCQ2672413.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            9145..10422
                     /locus_tag="OHO85_003070"
     CDS             9145..10422
                     /locus_tag="OHO85_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2672414.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            10419..11423
                     /locus_tag="OHO85_003071"
     CDS             10419..11423
                     /locus_tag="OHO85_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCQ2672415.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            11420..12385
                     /locus_tag="OHO85_003072"
     CDS             11420..12385
                     /locus_tag="OHO85_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCQ2672416.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(12359..13105)
                     /locus_tag="OHO85_003073"
     CDS             complement(12359..13105)
                     /locus_tag="OHO85_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCQ2672417.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(13157..13979)
                     /locus_tag="OHO85_003074"
                     /pseudo
     CDS             complement(13157..13979)
                     /locus_tag="OHO85_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
     gene            complement(14044..14844)
                     /gene="thiD"
                     /locus_tag="OHO85_003075"
     CDS             complement(14044..14844)
                     /gene="thiD"
                     /locus_tag="OHO85_003075"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCQ2672418.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(14841..15629)
                     /gene="thiM"
                     /locus_tag="OHO85_003076"
     CDS             complement(14841..15629)
                     /gene="thiM"
                     /locus_tag="OHO85_003076"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCQ2672419.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(15664..15760)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15852..16124)
                     /gene="rcnR"
                     /locus_tag="OHO85_003077"
     CDS             complement(15852..16124)
                     /gene="rcnR"
                     /locus_tag="OHO85_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCQ2672420.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            16245..17069
                     /gene="rcnA"
                     /locus_tag="OHO85_003078"
     CDS             16245..17069
                     /gene="rcnA"
                     /locus_tag="OHO85_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCQ2672421.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATLSHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFGGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            17288..17626
                     /gene="rcnB"
                     /locus_tag="OHO85_003079"
     CDS             17288..17626
                     /gene="rcnB"
                     /locus_tag="OHO85_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCQ2672422.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(17708..18742)
                     /locus_tag="OHO85_003080"
     CDS             complement(17708..18742)
                     /locus_tag="OHO85_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HCQ2672423.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKKEDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSPVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKIKSGALE
                     ATALINVKYD"
     gene            complement(18758..21238)
                     /locus_tag="OHO85_003081"
     CDS             complement(18758..21238)
                     /locus_tag="OHO85_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCQ2672424.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKHSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     STPRRQIYMSNSTTFDDQGLASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(21254..21928)
                     /locus_tag="OHO85_003082"
     CDS             complement(21254..21928)
                     /locus_tag="OHO85_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCQ2672425.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(22008..>22524)
                     /locus_tag="OHO85_003083"
     CDS             complement(22008..>22524)
                     /locus_tag="OHO85_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2672426.1"
                     /translation="VFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSVSIN
                     MPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDKAIA
                     SSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDV
                     LSSLVMRVAQD"
BASE COUNT         5695 a         5703 c         5194 g         5932 t
ORIGIN      
        1 ctacataagt aggaattacg gacattgagg cccttcaggg taactccatg ggggctttaa
       61 tatattatat tgaagatgcc actgtttagt tgaatattag gtatatgctc ttttttgaaa
      121 tttatcggtg gcagccgtta gtattcgctg tccccattgc aagctcctgg tggtaaccac
      181 tgaatcctcc atacttgaac tgacttttta tcctccgact ttcatcctgt tctgactcca
      241 ccttttgttt tctgctctac actatctaca gaccaatcat aaaggcacat acgatcatgg
      301 cagaatttcc cgccagctta ctgattctta atggcaaaag tactgacaat ctacccttgc
      361 gcgaagcaat tatgctgttg cgtgaggaag gaatgacgat ccatgtgcgg gtcacctggg
      421 agaaaggcga tgccgcacga tatgtagagg aggcccggaa gttgggcgtc gcaacggtga
      481 ttgccggtgg tggcgatggc accattaatg aagtttctac ggcgttgatt cagtgtgagg
      541 gggatgacat acccgcgctg ggaattttgc cattaggaac cgccaatgat tttgccacca
      601 gtgtagggat tcctgaggca ctggataagg cgctgaaact ggcaattgcc ggtaacgcca
      661 ttgcgataga tatggcgcag gtcaacaaac aaacctgttt tattaatatg gcgacaggcg
      721 gatttgggac gcgtattacc acagaaacgc cggaaaaatt aaaagccgcg ctgggtggcg
      781 tctcttacat cattcatggc ttaatgcgca tggatactct gcaaccggac cgttgtgaaa
      841 tccgcggtga aaactttcac tggcaaggtg acgccctggt cattggtatt ggtaacgggc
      901 gtcaggccgg tggcggtcag caattgtgtc cgaacgcgtt aattaacgat ggcttgctgc
      961 aactgcgcat ttttaccggc gatgaaattc ttccggctct cgtatcaacc ttaaaatctg
     1021 acgaagataa cccgaatatt atcgaaggcg cttcgtcgtg gtttgatata caagccccac
     1081 acgaaatcac ttttaatctt gatggcgaac cgttgagcgg acaaaacttt catattgaaa
     1141 tacttccggc ggcgttgcgt tgtcgattac caccagattg tccattattg cgttaatcaa
     1201 actattctct cttgaaccag ggagaatccg aaatgccgga tgcggtgtaa ataccttatc
     1261 cggcatacag aacaatacta caccgtaatt aattttaccc gtgtcttctg caatgccagt
     1321 ttatccacct ctgatacagc atcatcggta ataataatgt caattttttc gattggcagt
     1381 acctgattaa aaccgcgacg gttaaatttc gatgaatcga gaacggcaat cactttatta
     1441 gcggcggtga ccatcgcacc gctaatcgaa taaccttcat taaaggtcgt aataccatta
     1501 accgcatcaa taccatcagc accgacaaac attaaatcgg cattaatatc ctgcagggaa
     1561 cgttcagcaa tagatccatg catagatcgc gttttatggc ggaccgtgcc accgcagacg
     1621 accagagtaa tgtctttatt ttcggaaagg gcaaacgcag ccgggagact gttggtaatc
     1681 accgtgatat ttttagcggt cattaatcct tcggcgataa gcattgtggt acttccgctg
     1741 tcgagaataa cggtcatccc ttcatggatc attttgactg ccgcctgcgc tattctgttt
     1801 ttgggcgcgc tggcgagctg aaatcgctct ttaaacccga cttcctgggt ctcggtttca
     1861 ctattaccgg acattatttt cgccgcaccg ccatgaaagc gcgtaaccac gcctttttgt
     1921 tcgagaaagc gcaaatcggc acggattgtc gcttccgagg cagcaaatac tcccgccaga
     1981 tcctgaacaa gcacggttcc ctgttcattc actaattgga tgatcttatt ccttcgctcg
     2041 aatgaattca tcgtcattta cccatattca attgtggcta gtgtaaacga agggggacca
     2101 ttggtgaatg atctgttttg cacaataccg ggccagcatc acgctggccc gcggtttcat
     2161 ggaatgagca acactttgcc cggcatggca ttacgagcga tgtcacgcac cgcctgggtg
     2221 aagctttcaa agcttccacg gtgagcgatt aatggctcca ggcttaactt acgttctgtc
     2281 agcaaccggc tcgccgtttc ccactcctgc cccggccaag ggctggaata gttcatccaa
     2341 ctgccgataa ccgtcagctc tttacgcaat attttgccaa acgttgccga tgttaaatgc
     2401 agatcctgat gcaacgtgcc caccagcgcc agttgggcat gaggaccggc aatctctacc
     2461 gccagttcga cagtttgcgg tacgccagcc gtctcgagga taagctgatt aaaacgcagt
     2521 tcgcgtaaaa cgctctgcat ttgcggcgcg ctcatttcgc tactgttaaa tgtttgcatc
     2581 gcaccgaaag attttgccag tgccagtttt tctgagctaa tgtcgatagc cgtcacactc
     2641 tttgctccca gcgcgacagc gcactgaata gccagcaggc caatggttcc ggcaccaata
     2701 ataataacgt ttttattctc acaacctttc gctaaatgaa aagcatgcag atcaacggta
     2761 atcggctcaa taaaagcccc atcctcaata ggcatatccg tgggtagagc aaagacattt
     2821 tttcgcttaa cgacaatata ttcagcaaat ccaccatcac gccgcgagcc aataaaatca
     2881 tattttgcgc actgggaata aaaccctttc agacactctg gacaagtaaa acagggtaat
     2941 aacggcacac aggcaaccgc atcgccaggg tgtaaatcat caacaccgga tcccaccgca
     3001 tcaatatagc cgctaaattc atggcctaac gttattggat aataatgtgc accattttta
     3061 aatatcctgg gtaaatcgga accacataag cccgagctgg caatttttac ccgcacctca
     3121 tcctgatgtt taatttcagg aatgacgctt tctgcaacgc gcacgatacc atcagtatca
     3181 ttcaccactg atttcataaa aactcctgat tgttaagggg gataacctcc ccctcaggta
     3241 aaaattattc tgcgagaacg actttctctt gtttaataaa gccacgcgct ctacgccagg
     3301 tcatgaaaat accggtcaga taaattgcac cgataataat gaaaccggga atattttgcg
     3361 gggagaaaac ctgaatcagt aaccaggtaa tgggagaacc gccctgatcc attgaagcca
     3421 ccataccccc ggcttttaac gccccagcat tagccgccag ttgggtgtgc aaaccaatag
     3481 tttgcgtcgc gatccacaga gtgatgctca tgatgatgac acccgagatt aaggtgcgga
     3541 acagatttcc acgatgcacg gcgaccgcca tcgccacgaa gaagccgatg gtggcaagat
     3601 cgccaaacgg cagcacctga ttacccggca cacaaacagc aattaaaatg gtgagtggga
     3661 taaaaatcag gcttgccgat accaccgccg tatgtcccag cagcaacgcc ggatcaaggc
     3721 caatcaggaa ctcctgaccg ccgaacttcg cctgtaaacg actacgagcc tgcttagcga
     3781 tgggcgttaa accatccatg atgggtttaa tcacccgtgg cattagcagc atcactgccg
     3841 ccgtttttac cgccagctgc aatacacctt tgacatcgta acccgcgagg atgccgataa
     3901 tcagccccat cacaaaaccc acggtgacag gctcgccaaa tggaccaaat ttgcgctgaa
     3961 tatcgtcggc gctaaattta attcggttaa cgcctgggat tttctcgatg atagcatcga
     4021 ccagcaccgc aatcggcccc atatacgccg acgtaccgtg cggaatagca ataccttcca
     4081 gctcaaagaa atttcgggta tcgcgggcaa accagtcgcc gagcttataa acaaacgccg
     4141 cgtgaattac gacacctgcc atccctatca tccatgaacc ggttgccaga tgcagcaacg
     4201 cgccggtgaa ggtcatatgc cagatattcc agatatcaac atttaccacc cgcgtcatac
     4261 gggtcagtaa catcgccacg ttaaccaata tcgcaatcgg aatcgccacc agcgcaattt
     4321 gcgaagccca ggtcattggt gaagagcccg gccagccgac atcgaccaca tgcagattca
     4381 ggtcgaaatt ttccgccatc gctttcgccg ccggaccaat ggaatccagc attaagccaa
     4441 tcacaaggcc aatgccaaca aagccaatcc cgatatgcag acccgcttta aagcaatcgc
     4501 ctgccttcat gcctaatatt ttagaaaaaa taatgatgac aatcggaagc atcaccgtag
     4561 ggccgaggtc gagaatataa cgcatgactt ctgaaaacat aggtcacccc tgtaagatag
     4621 tcagaatttt attttgtaat gcttcgatac cgacaccaga aataaaaggc atgccgtgaa
     4681 ctaacggaat atcgccaaaa ctacgatcca ctttggcagt ggtgcatatc aaatgcacac
     4741 catccatata ggtttctatt tcattaaccc gacactggat taattcaaca ggaatattat
     4801 gattctgaca caactcttta atttcttccg ccgccatcgt agaggtcgca accgcgcctc
     4861 cgcaagcgac aataatcttg cgtttcataa atagtccttt ttattgttac agagggattt
     4921 attcgtctga atctaaaaca tactttgtga agtattcctt taactgagtt tcaggaagag
     4981 taattagtgt ctcgacgata tcgggctgtt gtaacttgcc aaataaacag cgtaaaagtt
     5041 tcaattgctg ctgcggattt tccacaatca acgcaataac caacgacacc tccacgtcgt
     5101 tatcatcatc cgcttgctga aaatgaactt tatttgttgg ccttaacaga taaatggctg
     5161 acgacttagc atgaatcgcc tcacaatgcg gtatagcaat ggcgtgctgc tcaagcatta
     5221 tcccggtagg gaattctgct tctctggcaa ttaatgcctg tggccaggta tcataaacca
     5281 cacctttcgc gagcatctca ttaccgatat gggttaaaac ttcgctacga tcgacaaaag
     5341 aaattccgct acgaacaaac aggttagtca tataccgtcc ttattccgca cagccgtagc
     5401 gataggcgcg caaaacatca taaattttat ccataatcag ctgatgcggt attgctgata
     5461 attccccgga ctgaatgcgt tcaaattgtt tgggaagata ctgactaatc atgcccagtg
     5521 ggatgtccac gccttcaaga ttcaccatca tcgtttcgac gctattttta atccgactat
     5581 gcggccagta ataacgaata cgatccgaca ggctgtaacg aatatccagt aatgaatcgt
     5641 taaaacccgt acgataatat tttttccagt attgcggttc gtcgagcatc acttcttcaa
     5701 ttaccgccag gcaaccgctg cgattttcag gggcgataag ttcctgctca atttgtgcca
     5761 gtgcaaatat cgcctcgcgt aaagcaaagg ttaatgcggg accgactttc aatattgcaa
     5821 agtgatcgcg gactaattcc caataagccg tccgggtctg gtaatcggta gaatgtgctt
     5881 cataaaccat tcgggtgctt tctatccatt gcgccagcgg ctgcgcttcc tgcggctgat
     5941 aatggataat attgctgtga tcaaattcca cgcccggctg caccacgatg gcaatcacgc
     6001 gtgttaacgc ctctgtcagc ccacgggcaa taaaggcctt ttgatgcgta cgtaaagtat
     6061 tggcggcatc ttcaacatgg gtgatgtgta ctgactgaat ggcgctggcc tcaccgcccg
     6121 gaaccggaac ttcggtgcca atgacatagc tcagttgctc acgctggcaa tctgtcgcca
     6181 cactttccgc agcaaagcaa agcacagcag ctcgttccgc aaccgtttct ggtgctaacg
     6241 gtatgggatc ccccgcgcag gacattgacg catcaagatg aattttactg aaaccggcac
     6301 gaacatattc ctttaccagc tcgacggatt tttccatcgc cgcatccgca ttttcttgct
     6361 gccagcagtt tggccccaga tgatcgccgc cgagaataat gcgttcgcgt gcaaacccaa
     6421 ctttatcggc aatcgtaaaa acaaattcgc gaaagtctgc cggtgtcatt ccggtataac
     6481 cgccaaattg attgacctgg tttgacgttg cttcaatcag cactttgcgc gtgctgttgc
     6541 gatcaaatgc cagcgccgct tcgataacca acggatgggc agaacagact gaacatatgc
     6601 cgatatgttc accagcttta tgccgggcaa ttaacgtttt catgtttttt ccttattaaa
     6661 tggcaggtgc gttatgccct gccctcgcag ccacaatcgg caatcacttt gctcaccaca
     6721 tcgcgcattg cggatttagc cgactgcaaa taatcccggg gatcggtcgc ttcagggtgc
     6781 tcggtcaggt aattttttaa cgcctgcgag aaggcatttt tcagctccgt tgcaacgttg
     6841 attttgcata tccccagttt gatggtttgc tgaatatcct tagtcgataa ccctgacgcg
     6901 ccatgcagca ccagcggtaa gttcacccac tggcgaatgt tctccagtcg agaaaaatca
     6961 agcgccggtg cgctggcata catcccatga gccgtgccga tggcgaccgc cagggaatca
     7021 attccggttg cctcggcaaa ttcacgcgcc tgagcggggt tggtataaaa agcatcggct
     7081 tcattgactt gcacatcatc ttcctggccg ccaagttgcc ccagctccgc ttcgacgctg
     7141 acatcaaagc gatggcaaaa atccaccacc tctttgaccc gtgaaatatt ttgcgcaaaa
     7201 ggcaaatgcg aggcgtcaat catgactgag cgcacgccag aacgaacctt ctgagcgata
     7261 tcgtcaaatt tcgtgtgatg gtcgagatga attgccagtg gatggtgata ttgcttcgcc
     7321 atcgcgctga ccagcgccaa cagattttct gtaccagcat gagtaaatgt gccaggcgtt
     7381 ccggcgatga tgaccggcgc atgcaggttg gcagcggttt ccaccaccac ttgcatcgtt
     7441 tcgagattgt gaatattgaa tgccggaacc gcataaccgc cgcgctgtgc gttgttcagc
     7501 atctgctttg tcgataccac gtacattttc atatcctgtc gtttgttttc gatttcaaaa
     7561 tataatgaaa tgatttgttt ttaaatgtcg agataacgat cacaaaaacg acaatgtgaa
     7621 aattgttcga ggagtaaaag gcaaaaaaaa acggcctccc gatagggaag ccgtagcaaa
     7681 gtgcgcgtgt ttttatgccg gatgcggtgt aaacatacgt ggcagtgcta tgtaggcatg
     7741 ataagacgct tccgcgtcgc atcaggcata ggttgccgga tgcagtgtaa acgccttatc
     7801 cgtcctacgg ggtggtgctg ttcgtatgag gcgtatcagg caatagtaat tttgctatcg
     7861 agataaacgt cctgcacggc attaatcagt ttcacgccgt cagccatcga tttcttgaac
     7921 gccttacgtc caagaatcag ccccattccg ccagcgcgtt tgttgataac cgcagtacgc
     7981 actgcatcgc tgaggtcagt ttcaccgccc gcagcaccgc cggagtttat caacccagcc
     8041 cgacccatat agcagttagc taactgataa cgcaccagat caatcgggtt ttcgctggtc
     8101 aatttgctgt aaacacgatc gtcggtgtaa ccgtaattaa ttgctttata gccgccgtta
     8161 ttttccgcca ttttttgttt gacgatatct gcaccgatgg ttgccgccag atggtttgcc
     8221 tgaccggtca ggtcggcgga aacatggtaa tcaacgccat ctttcttaaa ggcggagtta
     8281 cgcaaatagg cccacagcac tgtcaccata cccagctcgt gcgcacgttc aaaagccgca
     8341 gaaatttctt caatctggcg acgtgactct tccgagccaa aatagatagt cgcaccaacc
     8401 gcaaccgcgc ccatgttgaa cgcctgctcc acgctggcat acagcgtttg atcgtaggta
     8461 ttcgggtaac ttagcgtctc gttgtgatta agtttgacga ggaatggaat gcgatgcgca
     8521 taacgccgcg ataccgacgc cagcacgccg taagttgacg ccacacagtt acagcccgct
     8581 tcgatcgcca gttcaacaat gtttttcggg tcaaagtaga gcgggttagc agcaaatgaa
     8641 gctccggcag agtgctcaac gccctggtca accggcagaa tagaaagata tcctgtgcca
     8701 gccagacgcc cggtgttgta caacgtctgc atattacgta acaccgctgg cgggcgatta
     8761 ttgtcaatca ttacgcggtc tacgtagtca tgtccgggga gataaagctg gtcagaagga
     8821 attgtcatac aacggtgctg taaaaggttg tcggcgtctt tgccaagcaa ctgcgcaata
     8881 tctgtcatta ctatgctccc gtaaattccg attggatatc ggctatggat tgtcctggcc
     8941 cgccttttgc gggcaatcat aatcctggtc gttacgggcg agtttttcca tcttttggat
     9001 gttttttcag cgttttctgt tggctcgatt catcagaaaa aatgttagcg cggtcaagtt
     9061 ttcaccgcaa aggtatttaa aaggtattat taagtggtat tgtcatcgcg taccttacat
     9121 tacctgtcat gaaggaatta aaagatgaaa acaacagcaa agctgtcgtt catgatgttt
     9181 gttgaatggt ttatctgggg cgcgtggttt gtgccattgt ggttgtggtt aagtaaaagc
     9241 ggttttagtg ccggagaaat tggctggtcg tatgcctgca cagccattgc ggcgatcctg
     9301 tcgccaattc tggttggctc catcactgac cgctttttct cggcgcaaaa agtgctggcg
     9361 gtattgatgt tcgcaggcgc gctgctgatg tatttcgctg cgcaacagac cacttttgcc
     9421 gggttcttcc cgttactgct ggcctactcg ctaacctata tgccgaccat tgcgctgact
     9481 aacagtatcg cttttgccaa cgtgccggat gtggagcgtg atttcccgcg cattcgtgtg
     9541 atgggcacta tcggctggat tgcctctggt ctggcatgtg gtttcttgcc gcaaatgctg
     9601 gggtatgccg atatctcacc gactaacatc ccgctgctga ttaccgccgg aagttctgct
     9661 ctgctcggtg tgtttgcgtt tttcctgccc gacacgccgc caaaaagcac cggcaaaatg
     9721 gacattaaag tcatgctcgg cctggatgcg ctgatcctgc tgcgcgataa aaacttcctc
     9781 gtctttttct tctgttcatt cctgtttgcg atgccactgg cgttctatta catctttgcc
     9841 aacggttatc tgaccgaagt tggcatgaaa aacgccaccg gctggatgac gctcggccag
     9901 ttctctgaaa tcttctttat gctggcattg ccgtttttca ctaaacgctt tggtatcaaa
     9961 aaggtattat tgcttggtct ggtcaccgct gcgatccgct atggcttctt tatttacggt
    10021 agtgcggatg aatatttcac ctacgcgtta ctgttcctcg gtattttgct tcacggcgta
    10081 agttacgatt tttactacgt taccgcttac atctatgtcg ataaaaaagc ccccgtgcat
    10141 atgcgtaccg ccgcgcaggg gctgatcacg ctctgctgcc agggcttcgg cagtttgctc
    10201 ggctatcgtc ttggcggtgt gatgatggaa aagatgttcg cttatcagga accggtaaac
    10261 gggctgactt tcaactggtc cgggatgtgg actttcggcg cggtgatgat tgccattatc
    10321 gccgtgctgt tcatgatttt tttccgcgaa tccgacaacg aaattacggc tatcaaggtc
    10381 gatgatcgcg atattgcgtt gacacaaggg gaagttaaat gaaaacagaa cgtattctcg
    10441 gtgctcttta tgggcaggcg ttaggggatg cgatggggat gccctccgag ctttggccac
    10501 gcagccgcgt taaagcacac tttggctgga ttgaccgttt tctgcctgga ccaaaggaga
    10561 ataacgcggc ctgttatttt aaccgcgccg aattcaccga cgatacctcg atggcgctgt
    10621 gtctggcgga tgcgttactg gaacgtgaag gcaagatcga tccggatctg attgggcgta
    10681 atattctcga ctgggcgctg cgtttcgacg cctttaacaa aaacgtacta ggtccgacct
    10741 cgaagattgc gcttaacgcc attcgcgacg gtaaacccgt tgctgaactg gaaaacaacg
    10801 gcgtgaccaa cggcgcagcg atgcgcgtct cgccattagg ttgtttgctt ccggcgcgtg
    10861 atgttgattc ctttattgat gatgtggcgc tggcgtcgag cccgacccat aaatccgatc
    10921 tggcagttgc aggtgcggta gtcatcgcat gggcgatttc tcgtgccatt gacggagaaa
    10981 gctggtcagc gattgttgat tccctgcctt caattgcgcg acatgcacag caaaaacgca
    11041 tcactacctt cagcgcctca ctggcagcac gtctggagat tgcgctgaaa attgtgcgca
    11101 atgccgacgg caccgaatcc gccagcgaac agctttacca ggtcgttggc gcaggtacca
    11161 gcactattga gtccgttccg tgcgccattg cgctggttga actggcacaa accgacccga
    11221 atcgctgcgc cgtcctgtgc gctaaccttg gcggcgacac agacaccatc ggcgctatgg
    11281 cgacggcgat ttgcggcgcg ttgcatggcg ttaacgctat cgatcctgca ttaaaggcgg
    11341 aactggatgc ggtaaatcag cttgatttca accgctatgc cacagcgctg gcgaaatatc
    11401 gtcaacaacg ggaggcggta tgagcggcgc tcgattacac acgctgctgc ctgaattaac
    11461 ctcgcgtcag ccggtgatgg tcgtcggcgc ggcggtcatt gatgtgatcg ccgacgctta
    11521 tgccctcccc tggcgtgggt gcgatatcga actgaagcag cagagcgtta acgttggcgg
    11581 ctgcgcactg aatattgccg tggcgttaaa gcgcctcggc atcgaagcgg gtaatgcctt
    11641 gccgctcggt cagggcgtgt gggcggagat tattcgcaac cggatggcga aagaggggtt
    11701 aatcagcctg atcgataacg ccgaaggcga taacggctgg tgtctggcgc tggttgagcc
    11761 ggatggcgaa cgcactttta tgtcattcag cggtgtggaa aatcagtgga atcgccagtg
    11821 gctggcgcga ttaaccgttg cgcctggcag cctgctctat ttttccggtt atcaactggc
    11881 ctcgccctgc ggcgaattgt tagtggaatg gctggaaaag ctgcaagacg tgacgccgtt
    11941 tatcgatttt ggcccacgta tcggcgatat cccggacgca ttactggcgc ggatcatggc
    12001 ctgtcgacct ttagtatcac tcaatcgtca agaggctgag attgccgccg aacgttttgc
    12061 tttatccgca gagataacaa cacttggcaa gcaatggcag gagaaatttg ccgcgccgtt
    12121 gatcgttcgt ctcgataaag agggtgcatg gtatttcagc aacgacgctt ctggctgcat
    12181 tccggcattt ccaacgcaag ttgtagacac cattggggcg ggcgacagtc atgcaggtgg
    12241 cgtacttgcc gggctggcct ctggtctgcc actggcggat gccgtattac tgggcaatgc
    12301 agtggcgtcg tgggttgtcg ggcatcgggg cggtgattgt gcgccaacgc gcgaggaact
    12361 actcctcgca cacaaaaacg tatagatcgc tgcgacagtg gctaatgctg tactcaatag
    12421 gccgttgctg ttggtcaagc gccacttgct tgatcaccag caccggtatt ttgctatcta
    12481 actgaatatg tgactgaaac tcggcatccg gcatccgggc gctaacgcgg gaacgcgtac
    12541 gttgtgggta aatgtgctgg ctgcggaaat agtcataaag tgaaatcccg atggcatcaa
    12601 catcatgaat taaatgcgcc ggaacccacg attcctcaat cgataccgct tcttcatcga
    12661 catagcgaat acgcttaagc aaaaagacat cacttcctgc ttcgaccgcc agctgctgcg
    12721 cgacttcttc gggacatttc acgacacgct tgttaaccca tagcgtatcg ggctttttcc
    12781 cgcgcaatac cacctgctga gaaaaacctc gcgcttcttt caacgagtat tcgaagatgt
    12841 tgttgatctg cgtgccgtaa ccgcgcgaac gcgtcaccac accctcttct tccagcgcct
    12901 gcatcgcctt gcgcaccgta atgcgcgaaa cgccggttaa ctgacttaaa tcacgctcac
    12961 cgggcaaaat attgccatgc tccagcaccc cgctgcgcac ggcatttttt accgtttcgg
    13021 caaacttaat ataaagcggc gtgttatccg ccgctaaaat acgctcgttc agttgggcaa
    13081 tcagctgggt atgcgcttgt tccatttgtc tttttcctgc cgaggagtct ggtgggagta
    13141 tatcactgct tcttatttaa ggcgtttctt taatcccatc aacgaatgcc tgcaactcct
    13201 ccaccgtgcc atgaaataca ttaaaatcca ccggaccatt gatgccatct acctgtccac
    13261 ggtcggaatg ctgccagaag cgccaggcca tgccgtcatt gtccggacga cgttgataat
    13321 agtgagccac ccaccacgga tattcattga aatagcccgc cagattggtg tgataaaaaa
    13381 cggctcctga gtaaataatc ggcttttttc ccgtactttt ttcgaccatt tttagccact
    13441 gacttactcg tttgcgtaat tcttttgccg ataattttcc ccgttcttct acgtccagta
    13501 ccgcagggaa atcgccttgt gagaaatcca ccgtttgcag aaataatcgt gcctgaaccg
    13561 gcgctgctac cgacggggag aaataatgat acgccccgcg cagcaggcca ttttcgcggc
    13621 ttagttgcca gttacgcgaa aaatacgggt ccaccagctt ttcgccttcc gtcgccttaa
    13681 taaataaaag caaactgtaa gcggataccg ttgtcgcgca tttttgccac ccgctgccag
    13741 tcgatccgct cctgccagcg agaaacatct attccgtgaa tggtgtaact ggcaggaatg
    13801 cgaatagcga aagatttaac cgggcgataa ccgtagaaat ggatgtagtc tgtaatctga
    13861 actgctgtcg aatagaaaaa attcaccgtc tgacgcggat aaattgcaac gatggaaatt
    13921 agcccaagag cgcataatag agaggtgagt ttttttcgac tggtaattct tagctgcatt
    13981 ggtttcatcc ctgaatgtca gtgccaaagg ctgacaataa ccaaagcagc tatagtacgg
    14041 tgctcaccac caggcgtgga agtggtgaac cggaccaata ccgtgaccaa cttccagcgt
    14101 gtcggcctgg gctaatgctg atgaaagcca gctttttgcc tcctgtacgg tgtcagccca
    14161 gtttgtatgg cgcgggcgta gtgcagccaa cgccgcagag agcgtacaac cagtgccgtg
    14221 ggtgtttttg gtcataatgc gcggtgcggt aaaccgttgt tcaccctcgc gggtaaacag
    14281 ccagtccggg ctttgctcat catccagatg accacctttc attagcactg cgccacagcc
    14341 catcgccaac agcgatcgcc cttgttccag catttcctgt tcggtgcgcg cgtgtggcgc
    14401 gtcgagcaag gcggcagctt cgggcaagtt tggcgttatt aatgaaacct gtggcaataa
    14461 tcgactgcgc agcgtagcaa ccgccgaagg tgaaagcagc gggtcgccgc tttttgccag
    14521 cataacggtg tcgagtacca cgttttggat ctgataacgt tgcaaccgtt ctgccaccgc
    14581 ttcaacaata tcggtttccg ccaacatacc gattttagtg gtgtcgattc gcacatcgct
    14641 gaacaccgaa tcgagctggg cggcgacaaa atcaggctca atgcgataca ccgactgtac
    14701 gccacgggta ttttgcgcca ccagtgcagt aataactgag caaccataag cgccaagtgc
    14761 cgagaaggtt ttaagatcgg cctgaatccc cgcaccacca ctgggatcag taccggcaat
    14821 cgtcagagcg ttaattcgtt tcatgcctgc acctcctgcg tcaattgcca gagcgcatca
    14881 aggaaatgtg gaacaaaact gcctggcccc tcgcttctgg cgactgcgcg ctctccggct
    14941 tgtttcatcc agtgacaggc agatgcgaca ttttccagcg tatcgcctgg taacgcacag
    15001 caggcagcga caaccgccga taatgcacag ccagttccta ccactttggt cattaacgga
    15061 tcgccaccgt gaatgccaac ggcgcgatgg ccatcggtaa cataatccac ctcgccagtg
    15121 accacgacga ttgcgccagt ttcccgtgcc agtgtttgtg cagcgggtat cgcgttagct
    15181 gcggcgtcag tggtatccac tccccgtcca ccattagcaa tgccagctaa tgccatgatt
    15241 tccgaagcat taccacgtat cgctgccggt ttaaaagata aaagttcatg acaaaaatgg
    15301 cggcgataat cgagcgcacc aaccgctact ggatcaagcg tccagggtgt ttgagagctt
    15361 tttgcttgct caacggcagc acgcatcgcc tgagcgcgtg gctgcgtcag tgtgccaacg
    15421 ttaatcaaca aggcactggc gatagccgca aactgactgg cctcttcggt ttcgataacc
    15481 atcgctggcg atgcaccgag cgccagcaag gtattggcag taaaggtttg caccacatcg
    15541 ttggtcatgc agtgcacaag aggggaatgt tggtgaaaaa ggtgtaacgc gtgcgcagat
    15601 tgcgctgaac tcagcaggtc gacttgcata gtttgctcct gccataacgt gaagaagcaa
    15661 tgacctggta gtccgtgact tccctacgct ggcattatcc agatcaggtg atacgggtat
    15721 ttctcagcct tcacgcagaa gggcaccccg agtcgtttgg ttgcgatgat aaatcgcaga
    15781 ggaggatggt aatgtccagc gcacgcgttg taaacgagga aaaacggcag ttttacaatc
    15841 gcgttaataa attatttgat atatgaatcc agcaccttca gaacgacatc cagatcttct
    15901 tcacgtttta gctcatcccc ctggtgaacg atgtgttccg tcagatgacc tttaatcact
    15961 tcccgcatca gaccgtttac cgcgccacgg atagcagcaa tctgttgtaa aactgcagcg
    16021 cattcgtgcg gctcgtcgag cattttcttg agcgccacga cctggccctg aatcttactg
    16081 gcacgcgctt tcagtttctg tttatcacgg attgtatgag acatggcaac acctggttaa
    16141 caagaatatg aaaaatcata gcactattaa tctactgggg ggtagtatca ggtactgggg
    16201 gggagtagaa tcagattgcc gaattaatac taagaattat tatcatgacc gaatttacaa
    16261 ctcttcttca gcaaggaaac gcctggttct tcatccccag cgccatctta cttggtgcgc
    16321 ttcatggcct ggaaccaggg cactcaaaaa cgatgatggc ggcgtttatc atcgccatca
    16381 aaggcaccat taaacaggcg gtgatgctcg gactggcagc aactctttcg cataccgcag
    16441 tggtctggtt aattgccttt ggcgggatgg tgatcagcaa gcgctttact gctcaatcag
    16501 cagaaccgtg gctccagctg atttccgcag tgatcattat tggcaccgcg ttctggatgt
    16561 tctggcgtac ctggcgcggc gaacgcaact ggctggagaa tatgcacgag tatgatcatg
    16621 aacatcatca ccacgatcac gaagatcacc acgaccatgg acatcatcac catcacgaac
    16681 atggcgagta tcaggatgcc catgcacgag cccatgccaa tgatattaaa cgacgctttg
    16741 gtggtagaga ggtcaccaac tggcaaattt tgttatttgg cttaacaggt ggtcttatcc
    16801 cctgtccggc agcaattacc gtgctgttga tttgcattca gttgaaagcc ctgacactgg
    16861 gcgcaacact ggtcgtcagt ttcagcattg gcctggcgtt aacgcttgtc accgtaggcg
    16921 ttggcgcagc aatcagcgtt cagcaggtcg caaaacgctg gagcggattt aacactctcg
    16981 ctaaacgcgc cccctatttt tccagtctgt tgattggctt agtcggtgtg tatatgggcg
    17041 tacatggctt catgggcata atgcgataac tcaatccttt aggcttgcat gttatcttca
    17101 gcccaggatg attcttaaat cagctatttc cgctgactct ctgctcgcta aggatgcttt
    17161 tagggcatcc tttcatttac actttttacg aaatcatggg atcactaaca aaatatcgct
    17221 tgtcagttat attgtatggc aggaaagata tgcgactgat attacagatc cccaaagtgg
    17281 agagtttatg accattaaaa ataagatgtt gctgggtgtg cttttgctgg ttaccagtgc
    17341 cgcctgggcc gcaccagcca ccgcgggttc gaccaatacc tcgggaattt ctaagtatga
    17401 gttaagtagt ttcattgctg actttaagca tttcaaacca ggtgataccg tcccagagat
    17461 gtaccgcacc gatgagtaca acattaagca gtggcagtta cgtaacctgc ccgcgcctga
    17521 tgccgggacg cactggacct atatgggtgg cgcgtacgtg ttgatcagcg acaccgacgg
    17581 taaaatcatt aaagcctacg acggtgagat tttttatcat cgctaaaaaa agccccctca
    17641 tcatgagggg gaaatgcaga caccttgtta ttttttatta ttagccactt gctcgtcttg
    17701 cttgttatta gtcgtatttc acgttgatta atgcggttgc ctccagtgcg ccagatttaa
    17761 ttttatttgt atcgtagacg tagtaactgg ctgttatcgg aattgaaacc gttgtatctg
    17821 taagttttga ctttgccgca atttctttta aatttgtgta tcctttactg ctgttagtga
    17881 tatttaaaag tgtgactggc gaaccattac tttcaagaat aaatcctacg ccattatctt
    17941 ccgcaccttt cagtattgtg ttgctgttgg ttaacaagtc agaacttgat aaatacacct
    18001 gcattttccg tgatgtttga ttttgttgac ctaataaatt tttacaacgc agaataatat
    18061 cctctttttt actatttatt gttgctttgt tacctgtggc tggtaattgt gaaacgggaa
    18121 tactgtctaa agtaattaat aaatcgtccg gtttgcaggt tgtggttatc gggttgtatg
    18181 acaatgtcag attaaaggaa tagacacctg cattttgtgc caatgcattt ctgtagctat
    18241 ttacacatgc atcccatgac ttgccaccta ataaacacag tagaaattgt actgcggcac
    18301 tcccgcgtgt cgatgatgaa gcactggata atgtcgcaac accattaatc atcactgaac
    18361 cgccgccccc cgcagaaaca ttcacttttg cattactggc cgcaggtgtc accgtaactg
    18421 aataaccact ggtgaaactg gtctgattat ttgacaacgt gatgttgttt ttattggtcc
    18481 attcaaaatg taaatttaat tttatggtat cgttataaag cccaacaata atatccgacg
    18541 aactcatagg ggtgatagta tttgtactca cgcatgacga atcattaaaa ttatgctcat
    18601 atgtcgctat ttgtgtttga gtaagtgcaa aatccccctg aacaaccaaa ttgtgctgaa
    18661 cagataactc actataacat gccgcatagc tcacaggcat aaccatcatc attaatataa
    18721 atagcattat gcgaatttcc atagaaccac ctgactatta ctggcaaata taatttctac
    18781 tttcatcaat ctctttaccg aaggtgattg tgcatgaaag tccttgttgc ttatcaattg
    18841 ctacattaac cgatggcggt atttcattgg tgcgaataaa taactggctt ccctggccga
    18901 caacgccaat attatgaccg tggatatcat tgacttcata accaaacgtt aatggttgcc
    18961 catccgctct taacgctttt ataaaccagg gcttgcgctg atcggtatca aaattaacca
    19021 gtacaaccgc gccgcgataa ggtgcggcaa ttttccggtt gccacgtaat tctgcttcgc
    19081 tatcgctttg cgacacatcc agcatcaggt gattttcccg ataaggtgtc attccgtcgt
    19141 ataccaccac tccattacgg tttgttgtgc gatatttttg cccattgaca taagcatctt
    19201 taattcctgg cgcattcatc acagcaaacg tttcggaaag acggttcgcc agattaacgc
    19261 cacccgacca ggcgacaatg ccccctgaaa cactggctcc agcctgtcga taagtactcg
    19321 actgactata actgccattc actgttgcca ccggcgcgtt ccaggttaaa ttcgctccag
    19381 ctgtcgtttc atttccctga tgttgatgac tcaggttgac accataattg aactgatccc
    19441 gactccctac tgttcctgat aatcccgtat tatttgaggc aagcccctga tcatcaaacg
    19501 tcgttgagtt agacatatat atttgccgac gaggcgtcga aacgtcatca ccccaatcaa
    19561 agggaatcga tataaaaata ttaaaacgtt tctcttcatg atgattctcg tcataagcct
    19621 ggcttgccgc gagggtatag cttatccgtc gcaggttgtt ggaataactc aactgataat
    19681 ccttactact gccgctacgc ccccagtaat ctcgccataa cgtacttaat gacacagacc
    19741 cccaaccttc tggcaatgac tggctcatat tggcggaaaa gctatttttg cgaccaaaat
    19801 cattctggta ataatcggca atgtcataga catcgttttc atcacggcga taattatctt
    19861 tattgtttgc ccaaacgtga tcgttaaatg tccggtaatc acgcgacgaa taacgccagg
    19921 ccgccagacc aaaacgcgtc gacgtttggc tcacaaattt gttgtaggca atttgataac
    19981 tttgcccgtc aaacacatcg ccgttgtctt gtttactatg cgacttcgtg gcatcgacgg
    20041 aaatggcacc aatgcgtgta ttccagccag tccccaaagt gaacgcgtaa taattattcg
    20101 caaccatcga gccaccatac aacgtcaata agttattaaa accatactga taacccgcct
    20161 ggacaaaatc actatgtttg ctcgcccctt caatatggct acgacccgcc gcaaaatcat
    20221 atttcgacac gccgggttgc agcatatttg gcaccgctgc ataaggcacc agataggtgg
    20281 ttaccgaacc gtccgcctct ttcacgctga catcaagatc tgctccacca ccggccaact
    20341 gcaaatctgt aatcgcaaac gggccaggag gaacctcttt ctgataaacc acaaaaccat
    20401 tctgttcaat agttaccagc gcgttactct gagcaatccc ctgcacccgt ggcgtaaaat
    20461 tttgtttcga gttaggcaac atctgcatat cacgaaacaa ccgcacacct ctgaagcgaa
    20521 cagaatcaaa aatatcgctt gatgtgtaca tatcacccac gcgaagcgtg ccgagaagtt
    20581 gggcaaatcc acgttccaga tacagggtat tgcttttcca cacccctgga ttgttatttg
    20641 ttttactgaa actggcatca gaatgcagtt gccaccccag taaatttaac ccgctgttaa
    20701 aacgtacata tgtactctta ctattacccg acgctttata gtcactgtaa tactgactca
    20761 cataataaga ggtataaaac gcattaatac cccgctccca gttttccggt ggaacatagc
    20821 cactttccag ttcttccacc caggcctgcg ggacactgaa atcgagacga aaaaccccga
    20881 tatcccaggt atagctccca ccctgaacaa gttgctcaaa tgttaaacat tgcttaccgc
    20941 tggcgaagtt atcgctatta atgcctaacc gcttgataac ttctcttgat aaacatgttt
    21001 cttgcgggtt gtctttaaca ataatctcat atttcccgcg ccattgctta ttgacataaa
    21061 tatcaatgtc atactgcccc ggtaagggtt gattatcatc aagacgaata tttgcaacct
    21121 gctggtcttt cattccacct atcataaaat gggtatcaaa ggtttcttca gctgcataag
    21181 cttcaatgcc gagcaataac gctacgattg ctgatgcaag tggggtcatt ctcaacatag
    21241 gcagctcctg caattaaatt ttgtcactaa tatagttgcc gtggtcatcg ataatggtca
    21301 gataccagtt atttgcatta ttacttttga cattaacact ctgactttct aagggggcaa
    21361 tcattatagt ttcataattg actttgacat tattagcttt gacatccgaa atcgtcaccc
    21421 aattagctga gtcatttttt atcaccaatc cattgccact gcgatttacc agtaactttt
    21481 taaaggtcgc tttatttacc ggagcaatac ccgccggccg gtaaaacaat ttgattctgt
    21541 tttgcatcgc aaacttcagt gcattcttac cttcttgttc tggactattt ggtggaatat
    21601 ccagaacatt cagataaaaa atgctttctt tattagtggg cagtttattc ggcataattt
    21661 tgatttttac ttgctgcccg gaatttgccc ctatttttgc aactggtggc gttaacatga
    21721 aaggaacctg aattttttct ggtggtaacg acgtatcgcc atcatcaatc cacgcctgca
    21781 gcagcgaaga acgatttccc tggttcatca actgcaccat aacttcttta ttttctgccg
    21841 ggtaaataat gcgcgtcccg tagataacaa ttccggcatg tgcaggaagg accatagaaa
    21901 aaatgagtaa agataataat cctttcattt taatgcctct taaattaaaa ggccgctatg
    21961 ggatagcggc catgtttatt acctggagaa acttgcaatt ttatttatta atcctgcgcc
    22021 acacgcataa ccagagaaga taacacatca cctgattgca ctgagttatc tgttagtttc
    22081 aggtattttg ctttaaacgg aatagcatat tgaccttccg ctgttttata actctcaata
    22141 ttaaaaggtg cagaaacatc caggacatcg ctgttatcac ctaaatagag atagtaaccc
    22201 acggtatcgt tgctactggc tatcgcctta tcgttgccgg ttattttgtc gttggtaatc
    22261 aacgagagtt ttacaccagc aacttctttc gggcagctaa tttttaacat gctgctggtt
    22321 acaccgactg catcatcata ggtagaatat ttagctttac cttcaaagac agtggttggt
    22381 acagtcggca tattaatact tactgaatca ccacccgctt cgaatttaca cggagattct
    22441 gcaatatttc ctttaatggt taatgttccg gtatcaacgt ctgcagcaaa tactccagcg
    22501 ctcataaaaa aagaagaaaa gaca
//
LOCUS       DAJOWB010000076        21882 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJOWB010000076 DAJOWB010000000
VERSION     DAJOWB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.076"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(15..416)
                     /gene="nikR"
                     /locus_tag="OHO85_003084"
     CDS             complement(15..416)
                     /gene="nikR"
                     /locus_tag="OHO85_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCQ2672427.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(422..1228)
                     /gene="nikE"
                     /locus_tag="OHO85_003085"
     CDS             complement(422..1228)
                     /gene="nikE"
                     /locus_tag="OHO85_003085"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCQ2672428.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(1225..1989)
                     /gene="nikD"
                     /locus_tag="OHO85_003086"
     CDS             complement(1225..1989)
                     /gene="nikD"
                     /locus_tag="OHO85_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCQ2672429.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(1989..2822)
                     /gene="nikC"
                     /locus_tag="OHO85_003087"
     CDS             complement(1989..2822)
                     /gene="nikC"
                     /locus_tag="OHO85_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCQ2672430.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(2819..3763)
                     /gene="nikB"
                     /locus_tag="OHO85_003088"
     CDS             complement(2819..3763)
                     /gene="nikB"
                     /locus_tag="OHO85_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCQ2672431.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(3763..5337)
                     /gene="nikA"
                     /locus_tag="OHO85_003089"
     CDS             complement(3763..5337)
                     /gene="nikA"
                     /locus_tag="OHO85_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCQ2672432.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(5448..6035)
                     /gene="acpT"
                     /locus_tag="OHO85_003090"
     CDS             complement(5448..6035)
                     /gene="acpT"
                     /locus_tag="OHO85_003090"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCQ2672433.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6090..7139)
                     /locus_tag="OHO85_003091"
     CDS             complement(6090..7139)
                     /locus_tag="OHO85_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCQ2672434.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7271..8488
                     /locus_tag="OHO85_003092"
     CDS             7271..8488
                     /locus_tag="OHO85_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2672435.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     SVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(8492..9049)
                     /gene="dcrB"
                     /locus_tag="OHO85_003093"
     CDS             complement(8492..9049)
                     /gene="dcrB"
                     /locus_tag="OHO85_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCQ2672436.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9122..9787)
                     /locus_tag="OHO85_003094"
     CDS             complement(9122..9787)
                     /locus_tag="OHO85_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCQ2672437.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10008..10253
                     /gene="tusA"
                     /locus_tag="OHO85_003095"
     CDS             10008..10253
                     /gene="tusA"
                     /locus_tag="OHO85_003095"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCQ2672438.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(10355..12553)
                     /gene="zntA"
                     /locus_tag="OHO85_003096"
     CDS             complement(10355..12553)
                     /gene="zntA"
                     /locus_tag="OHO85_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCQ2672439.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGNVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(12627..13253)
                     /locus_tag="OHO85_003097"
     CDS             complement(12627..13253)
                     /locus_tag="OHO85_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCQ2672440.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            13394..13753
                     /locus_tag="OHO85_003098"
     CDS             13394..13753
                     /locus_tag="OHO85_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCQ2672441.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(13756..14025)
                     /locus_tag="OHO85_003099"
     CDS             complement(13756..14025)
                     /locus_tag="OHO85_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCQ2672442.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14015..14611)
                     /gene="rsmD"
                     /locus_tag="OHO85_003100"
     CDS             complement(14015..14611)
                     /gene="rsmD"
                     /locus_tag="OHO85_003100"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCQ2672443.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            14761..16254
                     /gene="ftsY"
                     /locus_tag="OHO85_003101"
     CDS             14761..16254
                     /gene="ftsY"
                     /locus_tag="OHO85_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCQ2672444.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16257..16925
                     /gene="ftsE"
                     /locus_tag="OHO85_003102"
     CDS             16257..16925
                     /gene="ftsE"
                     /locus_tag="OHO85_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCQ2672445.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            16918..17976
                     /gene="ftsX"
                     /locus_tag="OHO85_003103"
     CDS             16918..17976
                     /gene="ftsX"
                     /locus_tag="OHO85_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCQ2672446.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18221..19075
                     /gene="rpoH"
                     /locus_tag="OHO85_003104"
     CDS             18221..19075
                     /gene="rpoH"
                     /locus_tag="OHO85_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCQ2672447.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19347..20450
                     /gene="livJ"
                     /locus_tag="OHO85_003105"
     CDS             19347..20450
                     /gene="livJ"
                     /locus_tag="OHO85_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCQ2672448.1"
                     /translation="MNMKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            20600..20866
                     /locus_tag="OHO85_003106"
     CDS             20600..20866
                     /locus_tag="OHO85_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCQ2672449.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            20873..21400
                     /locus_tag="OHO85_003107"
     CDS             20873..21400
                     /locus_tag="OHO85_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCQ2672450.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(21397..21780)
                     /gene="panM"
                     /locus_tag="OHO85_003108"
     CDS             complement(21397..21780)
                     /gene="panM"
                     /locus_tag="OHO85_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCQ2672451.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLCVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         5197 a         5738 c         6074 g         4873 t
ORIGIN      
        1 gtttcagaaa taactcaatc ttccttcggc aagcactgca aatgcccgtg ccgcacgccg
       61 cgctgggcga taacgtcatc ggcaaaatgc tgcacgtcgc ccatatcacc tttcagcacg
      121 gcgatttcca gacagtcgtc gtgattgata tgcacatgca gcgtggcgac ggagagatcg
      181 tggtgatggt gctgggtgga gacaatgcgg ctggctaagt cgcgtttttc gtgttcatac
      241 acataggaga gcaccgcgaa accttgcgtg ccgtgctgct gagtggcctc ctgggccagg
      301 gcgctacgca gaatatcgcg gatagcttcg gaacggttgt tataaccacg acgctggctc
      361 aggctgtcca gcgtctccag taaatcgtca tcaagcgtga tggtgactcg ttgcatttgc
      421 gttaaacctt ttctgtggtg cgacggcgca cggggaatgc gggtaatacc gcgttttgta
      481 gcacacgtcc ggcgtcagag gaaaaggtta atttatctcc caccacctgg gtttcgacga
      541 tttgtccgtt gtccatcacc atcacccgct gacagaagcg ttccaccagg cgtaagtcgt
      601 gggtgatgaa caggcaggcg gtgccaaact gttgttgtag ctttttcagc aggcgaatga
      661 cgcccgcctg taacacgaga tcaaggttag aaacggcttc atccagaatc agtagtttcg
      721 gttcgaccgc cagcgcgcga gccaggcaga cgcgctggag ctggccgccg cttaactgcg
      781 gtgggcgttt gtcgagaacg ctgtcatcga gatcgaccgc cttcagcatt tcgctggcgc
      841 gcgccagttg ttcggatttt ttcagtgata gcaggtggcg catcggttca cgcaggatct
      901 cgcgcacggt tttgcgcgga ttcacggcgc tgatggagtc ctgaaatacc atctgaatat
      961 cgcggcggaa cgctttacgc tgggcgcgat tgagtttcgc cagcggttcg ccgcgccagc
     1021 taatattccc ttgtgaggga gactctaagc ctaccagcag ccgcgcgagg gtgctttttc
     1081 cgcagccgct gcgtcccagc agggcgacgg tttcgccgct tttcagggtc agggaaacgt
     1141 tattcagcac cgcctgatgt tgatgttttc cgctaaatcc accgtgggca tagtgatggg
     1201 aaaggccgca gacgttaagt aaggtcatga tgccagctcc ataccgtaga gggcgagatg
     1261 agcggaaacc aggctgcgcg tcaccgtatg tttgggggcg ttaaacagcg tttctacatc
     1321 gccctgttcg acaattttac cttgtgacat caccgccacg tcatccgcca gacgcgccac
     1381 aacgcccata tcatgggtga ccagcagcat tcccggcgct tgtttttgca taatgctttc
     1441 cagcagatcg aggatgcgcg cctgtgctac cacgtcgagg tcggtggtcg gttcatcggc
     1501 gatgataaac ggtgattcac acagcaccgc catcgcaatc atcatgcgct gcaacatgcc
     1561 gccgctcatc tcgaacgggt acagcttcag cacgcgcgcg gcgttttcca gccccaccgc
     1621 ttctatggca gcggtaagcg tagcgtcatc ggcgggtttc cctaacgcca ggcaggtttc
     1681 ccgcgcgtgg gtgtgcatgg tgtgcagtgg attaaaggcg ctgcgcgggt tctgcatgat
     1741 ggtggcaatt ttgatgccgc gcagggcgca aggcgaaacc ggtttgccat cggctaaaat
     1801 ttcccccgcc gtctggcgaa cgccagcggg caaaatgccc agcgtcgcgg cgcaggttaa
     1861 tgatttcccg ctgccgctac cgccgactaa cgccagcacg cgcccgcgtt gcagggttaa
     1921 cgatacaccg tgtaccagcg gctgcgcggc ctgtagcgcg atattacgta gttcaatctg
     1981 ttgcggcatt agtgtgcgtg ctccgtcacc agatgaggat ccagatgatc gcgcagtgcg
     2041 tcacccacca ggttaaaggc catcacgctg ataaacagcg ccagccccgg ccagaacatt
     2101 tgcagcggct gggtccagat atactggcgc gcgtcgttaa tcatcacgcc ccattcggcg
     2161 gtcggcgcgg tcacaccgag gccaaggaaa gacatccccg cgacgtgcag catcatatgg
     2221 ccgatatcca gcgttgccag caccagcagc gaagggatca ccgcgcctgc cagatgatcg
     2281 acaaacaccc gcacatggcc cgcaccggaa agccgtgacg ccagcacaaa ctcgcgttgg
     2341 cgtagtgaaa tcaccaggct gcgcaccatg cgtgcatacc acgcccagtg cgacagggcg
     2401 atggcgataa ttacgttggt cagcccggtg ccgagcacgc caaccataaa gaacgacaga
     2461 atcgaggtcg ggaaggtcat aaacatatcg gcgacgcgca tggtggcctg atcaacgcgt
     2521 ccgccaatca gtccggcgct gccgccaatc accagcccta atgtcagcac cagcagcagg
     2581 caggccatta ccgaaccgag cgacacgcgg gtcgccgcca tcagccgcga gaaaatatca
     2641 cgccctaagt gatcggtgcc cagccagtgc tgcgcatccg gcgaaagcag gcgcgacggc
     2701 agatcaatag cctgagggtc atacggcagc caccactggc tggtaagcgc aatcagcgcc
     2761 agcagggcga taatgatcag cgccaggcgt accgaccagc gggaagagag gaaaaagttc
     2821 acgcgtgcgc tccttcatga cgacgaatgc gcgggtccag cgcggcgttg agcaaatcga
     2881 caatcaaatt acagaccaca aaaaccacca ccatcatcag cgtaaagcac tggatcaccg
     2941 gatagtcacg gttaaaaatc gccgacaccg catagcgccc gacgcccggc caggcaaaga
     3001 tgttttcgat aatcatcgtc ccgccgatca gttcgccgat gtgcatcccc acggcggtga
     3061 tcatcggcag cgaggcattg cgcaggatgt ggcgacgttc ggtctgtttg tcgttcaggc
     3121 cgcgcagacg cgcccaggtg acgtgacgct gaccggcgac gtccagcata ctggcgcgca
     3181 gtaaacgcgc gttaatcgcc agcgacataa aggcaatgga aaccgcaggc aaaatgatgt
     3241 gctgccagcc gccgtaaccc atcgcgggta gccattgcag atacaccgaa aacgccatca
     3301 ccagtaaaaa cgccagccag aagttaggca tcgacacgcc aagaaacgcg atgaaacgta
     3361 cggcgaaatc cggcagacgg tcgcggtggc gcgccgccca gataccgagc ggtacggaag
     3421 tgagcagaat taataccagc gccgcacctg caagttccag cgtggcgggc aggaagttca
     3481 gcatatcgtc cagtaccggg cgttggctgg cgaatgagat accaaagtca agatgcagcg
     3541 ccttccacaa ccaggtgccg tactggacgt acagcggctg atccagtccc agcatggtgc
     3601 gggtagaggc cagcatctcc ggcgtcggcg gcaggttaga cagacgcaaa taatcgagcg
     3661 ccgggtcgcc ggtaccgagg cgcagcatca gaaaaatgat caccgaggcg gcgagcacca
     3721 tcgggatcag cagcagaaag cggcgtaata cgtaacgcaa cattaaggtt tcaccggttt
     3781 aatctgttcg aacggaattt cggtggcgat cggcgcgtag gggatgttac ccagctccgg
     3841 ttttgatacc accatcatcg agatgtagct gataggcaga taaaccgcct cgtcatgcag
     3901 acgggtcaga atgtcgcgat acagcgcctg acgttgcgtt tcgtcatggg tcgccagcac
     3961 ttcgccgatc tctttatcaa tcagcggctt gtcggctaat ccttgctgtg cctggaagtc
     4021 agcgtgcgac ggtacgcgca ttgaactcag gaaggcgtgt ggatcgtatg gcgcgcccca
     4081 ggtgcggtgg aaaatcatgc caaaacgacc gtcgcgctga cgagcataga tactgctctc
     4141 ttcttcgcca atcagcgaga catctgcgcc aatctggcgc atatcagcct gaatgatttc
     4201 cgccatcgat ttgcttaacg catcggtgcc gatgaacgac agttcaatgc gcagcggctg
     4261 accatttttc tcgcggatgt ctttgcccgc aggcagcgtc caaccggctt tttccagcaa
     4321 ctctttcgct ttctgcggat cgtactggcg cggtttcagg ccgagattag cgtaaggcac
     4381 agaaggggca aacagggtgt cggcgacctg ctgggtgcca tataacgcgt tatcaatcag
     4441 cgattttttg tttaccgcgt aattaagagc ttcacgtact gccagctcgt tggtgggggc
     4501 tttggcggta ttgagcgcca gcatcacggt ttcgatcggc tgtgacagtt gggtgtggta
     4561 agccggattc tggctaaagc gggcgaaggt atcgagcggt aataaccctt cgtttccgta
     4621 cagcaggtcg atatcgccag tttcaaacgc caccgcgcgg gtagtcgggt ccgggatgac
     4681 gttaaaggtg atctttttaa tcgctggctt ttcgccccag tagttttcgt tacggacgaa
     4741 gacatcgtac tgattcagtt tcgattcctg caaaatccac ggtccggtgc caatcggcgc
     4801 tttaattccg ttcatggttt catggttttt aaactgcgag ggagcgataa aacggaaagg
     4861 acggggcagg gccagttctt gcaggaaagg atagtaggcg cttttcaggg taatttgcag
     4921 ctctgtttta ctgagtgctt taacatcaac aatctggttt gccagctcca gccaggcgtg
     4981 acgttgacgg ttatcgagca ctgcgcggaa gttttctgcc gccgcctcgg catcgaacgg
     5041 ttcaccgttg gagaatttca cgtcatcacg cagggtgaag gtccaggttt taccatcttc
     5101 tgaatgagtc cagctttttg ccagccacgg gatcaccgaa ccgtctgcct gatatttcac
     5161 caatggttca taaaccatgc tctgggcgaa catctggtta ggcgtgtaaa ggtgcgggtt
     5221 tagtggcccg acattcaccg gccaggcggt ggtgatttca tctggtgcag cggcatggac
     5281 gataaaagac gcacaagcca gcagcgcaaa tagagtgcgg cggagtgtgg agagcatggt
     5341 aaccccaatg gattaaaata gatggcggaa ataagtatga cgattttaag tattcgtcat
     5401 actgattacc tgttctggat caatagatgg gcagtcgggt gggcggatca gttaactgaa
     5461 tcgatccatt gcacactgtc ggcggtgagc gtaaaggggg tgggggtgca gatcgccagg
     5521 cttaaatttt caagctgaca atggctgact gacagcgagg agtgataggt gctgtctacg
     5581 ctgacgattt gccaggcgct gccgccacgc tgtttaacga tggcttcttt gcgcgtccag
     5641 atgcgccaga acatttccag ttgctgatca ggatgcacgg cgtccatctc agcgtgttcc
     5701 ccgaggctga atacggcgtt cgccagccag cgccagttgg cgcgcgggcg aatcacttcg
     5761 atatcgcagc cgacttcgcc ttcatcactc aacagcagag cgatatcgtc accgctatgg
     5821 cttaagttga accatagcgg cgtttccggc gcgaatgcag gtttgccttg ttcgccatag
     5881 atgatctccg gtagcgggga aagcgtgtgc gaaagcaatg cacgccccgc cagccagcgt
     5941 tcgcgtcgtg gaccttgcgg tgcttgctcg cgtaaacccg gtggcagtgg agctgcgctt
     6001 aaggtcgaaa ctttccccag aactatccga tacatatcag ggccaacgtt taatggaaaa
     6061 tgaaagtgcg tatcgtatca cttgtcgcct catcccggta accgactttt cggtctgccc
     6121 ggccccagta aaatcgccag tttgctgcca cctttcgtcg tttccatcca gattttacac
     6181 acgctggtta atggcaccga aagtagcatc cctaccgggc cgagcagcca tccccagatt
     6241 aacaatgaaa gaaataccac catggtggac atccccaggc gatggcccat catccgtggc
     6301 tctaaaatat tgccgatgac catatggaca actaaaaaca atgcgccgac cagaatacat
     6361 tcgtaaatac cattaaacag cagcacctga atcattggcg gtacggcgga aattaccgcg
     6421 ccgatattgg gcacgtagtt aagcaaaaac gccagtactg cccacatcag cgcaaactgt
     6481 acccccatca gctccagccc cagccagacg atgacacctg tccataaact gagtagcgtc
     6541 ttcaatgcaa gatagtgcga aacgccttta agtgcgcggt gtaatcccgc gatgtgaatc
     6601 tgtgggttat tcagcgcaaa acgcattttg taagggacgt ggcgcacttc aaacagcata
     6661 aaaactacgg tcatcaccag caaaagcacg ctcgccattg ccccggaaag cccggtcatt
     6721 agcgctgtgg tgaaggtaac cactttttcc gagtccatcc gctgcagcat tcgctccggc
     6781 gacatatgca aattaagaaa aggcaacatc tcctgcaatt taaaaagttt gcgcgtcaac
     6841 tccttattaa acttcggcag catagagata aattcgttaa acgatgccgc cagtacgccg
     6901 accagcgcag ttagtgcgat cagcatcacc accaccacaa tcgtaatggc aatggggcgt
     6961 tgtactcccc gacgaataaa ccaggtgacg agcgggttga ggacgatggc aaaaaacagc
     7021 gccagtaaca gctgcacaat gatatctgcc gctgcgtgaa tgcccgcgag gatcactacc
     7081 agcgaggcca gcttgagcag aatgtgcatg cccgttttat cgggttgagg ggtttccatt
     7141 ggggcttcct tgtgactttt tgtattaagt gtagcgggag tcacgccagc aatattctga
     7201 ttcttactgc tgattttcac gtcagcacgt ggtaaaaatg aaacactgtt gtaaaaatgt
     7261 ggtgatcctc atgcccgaac ccgtagccga acccgcgcta aacggattgc gcctgaattt
     7321 gcgcattgtc tctatagtca tgtttaactt cgccagctac ctcaccatcg ggttgccgct
     7381 cgctgtatta ccgggctatg tccatgatgt gatgggcttt agcgctttct gggcaggatt
     7441 ggttatcagc ctgcaatatt tcgccacctt gctgagccgt cctcatgccg gacgttacgc
     7501 cgatttgctg ggccccaaaa agattgtcgt cttcggttta tgcggctgct ttttgagcgg
     7561 tctgggatat ctgacggcag gattaaccgc cagtctgccc gtcatcagcc tgttattact
     7621 ttgcctggga cgcgtgatcc ttgggattgg gcaaagtttt gccggaacgg gatcgaccct
     7681 gtggggcgtt ggcgtggttg gctcgctgca tatcgggcgg gtgatttcgt ggaacggcat
     7741 tgtcacttac ggggcgatgg cgatgggtgc gccgttaggc gtcgtgtttt atcactgggg
     7801 cggcttgcag gcgttagcgt taatcattat gggcgtggcg ctggtggcca ttttgttggc
     7861 gatcccgcgt ccgacggtaa aagccagtaa aggcaaaccg ctgccgtttc gcgcggtgct
     7921 tgggagcgtc tggctgtacg gtatggcgct ggcactggct tccgccggat ttggcgtcat
     7981 cgccaccttt atcacgctgt tttatgacgc taaaggttgg gacggtgcgg ctttcgcgct
     8041 gacgctgttt agctgtgcgt ttgtcggtac gcgtttgtta ttccctaacg gcattaaccg
     8101 tatcggtggc ttaaacgtag cgatgatttg ctttagcgtt gagataatcg gcctgctact
     8161 ggttggcgtg gcgactatgc cgtggatggc gaaaatcggc gtcttactgg cgggggccgg
     8221 gttttcgctg gtgttcccgg cattgggtgt agtggcggta aaagcggttc cgcagcaaaa
     8281 tcagggggcg gcgctggcaa cttacaccgt atttatggat ttatcgcttg gcgtgactgg
     8341 accactggct gggctggtga tgagctgggc gggcgtaccg gtgatttatc tggcggcggc
     8401 gggactggtc gcaatcgcgt tattactgac gtggcgatta aaaaaacggc ctccggaaca
     8461 cgtccctgag gccgcctcat catcttaaaa cttactgaat aaccagcgta ttaatgatgt
     8521 tttctgcggt ggtctgcgct ttttgctgat cgtcagcggg cagcgtaatt tgcatggtca
     8581 gcagttgatt acccacgtta cccagaataa cggaagagta cgccgtctgg cctttcgcgg
     8641 agataatact gtctaactgc tgcattttgt gacctttcag ctcaatggct ttattggtta
     8701 ccacttgcag ctgcggatcg cggctacgtt gctgatcttc cagacgcttc gccagcaccg
     8761 ccagatcttc tttcggatca tcgcccatga tgacgatgac tgctttctgc ccggtggcgt
     8821 cggaccagac atgcatgtta ttggcctgcg ttcccagctt accgctctgg tcggtcatat
     8881 ccgctggcag cgagaaactt aacttgccat caagcaggtt gacgggattc ccggtagcgt
     8941 tactttccgc gaccgaaccc tgcgccgtag cgttagtgtc tttatcatca caggccgcaa
     9001 gccccataac cagcaggcca attccgacat atttaaccag attgcgcatt gacttcttcc
     9061 tttcgataaa cggccataac ggctcattca tccatcttat cacaactctg ataacgaacc
     9121 tttaactcgc ctgcaaagcg ttgatttcgg atttatctgc cagtcttttc aacagcatat
     9181 tgagtaatac gccatacatt ggcaggaaga aaacgatact gattaacact ttgaaacagt
     9241 aatcgaccag cgcgatttcc atccagtgtt cagccataaa ggcatccggg ctacgccaga
     9301 aggcaatgaa gaaaaaggcc agcgtgtcac tgacgttacc gaacagtgtg gacgctgtcg
     9361 gtgccagcca ccagcggcga ctttgacgca ggcggttaaa aacgtgcacg tcgaggattt
     9421 gccccagcgc gtaggccatg aaactggcgg tggcgatacg ggcgacaaac aggttgaagt
     9481 gggcgagtgc gccgaatccc tgccatgaac ccatatagaa cagcgacgag atgacatagg
     9541 agattaataa cgcagggatc attaccgcga agataatgcg tcgggccagc ggtgcgccaa
     9601 aaatacgcac ggtcaggtcg gtcgcgagaa agataaacgg aaaactaaac gcgccccagg
     9661 tggtatgaaa accaaaaatg gagaccggaa gctggaccag atagttactg gaggtgatca
     9721 ccagcagatg aaataacgat aaccagaaca acgccttata gcgttgagtt tgcgagaaaa
     9781 cgttcatatt gtaccttttt gattaaccat tggggtgagg gaacccaata cgtacgacac
     9841 gtctgttatc ttaacataat aacgtgctta cccttttttc gagccgccgc atgatactgc
     9901 tttacgttga caatgcaatg gttaattttc acgcaatcgt taacctggtt tgcttacgga
     9961 ctcacagggc gtaaactagc gccgtttttt taagtgatga gaagaaaatg accgatctct
    10021 tttccagccc tgaccacaca ctcgacgcgc ttggcctgcg ctgcccggaa ccggtgatga
    10081 tggtgcgcaa aaccgtgcgc aatatgcagc ctggcgaaac tttactgatt atcgccgacg
    10141 atccggcgac gacccgcgat attcctgggt tttgtacctt tatggaacac gaactggttg
    10201 cgaaagagac ggatggactg ccttatcgtt atttgattcg taaaggcggt tgatggggaa
    10261 agattggctt cgatgccgcc ttttcccctc accctaaccc tctccccaga ggagcgaggg
    10321 gaccgatcgc gctcaatgtt gcgatcggtt tgccttatct cctgcgcaac aatcttaacg
    10381 cattcgctgt taccagcacg gtcgcaccgg tatctgccag caccgccagc catagtccgg
    10441 tcatccctag cagcgtggtg acgaggaaga tccctttcag ccccagcgca atagtgatgt
    10501 tctggcggat attggcgtga gtggcgcggg ccagttcaat catttgcacc aggccgcgca
    10561 ggtggttatg ggttaatgct gcgtcggcgg tttccagcgc cacgtctgtg ccactgccca
    10621 ttgcaatccc aatggcggca gctttcatcg ctggcgcgtc gttaatacca tcaccgacca
    10681 tcgccagggg cgcatgttga ttcagcttgg tcaccgcttt gactttatct tccggcaata
    10741 ggcccgcttt aaactccagc cccagctccc cggcaattgc cgccgctgcg cgtggattat
    10801 cgccggtgag gatcactcct ttaaccccca gcgcgttcag ttcactgatg gcagttgctg
    10861 cgtcggcgcg cagggtatcc tgcaatgcaa tgatacccag cacgtcatca ttacgtacta
    10921 ccagcacaac cgtttgcccg gcgctttcca gttcattaat caaaccagca aatgcatcag
    10981 cgggatgttt cccggcagcg caaatcaata cgcgctcacc gttaacctgc gcttcaatgc
    11041 cagacccgac cagcgcccgc tgtgattcgg cggtgggaat ggcgagttca gcaacctgtg
    11101 cttcgcgtac gatggcctgc gccagtggat gcgtcgcgcc ttgctcgacc gccgccgcca
    11161 gtgtcagcag ttcagattca ctaatacccg ttgccggatg aatcgcggta acgcgcggtt
    11221 taccgacggt cagcgtaccg gttttatcaa acgccacctg agtaacacga cccagctgtt
    11281 ccagcgccgc tccgccttta atcaacgccc cacgacgcgc tgccgccgcc agcccggagg
    11341 taatcgccgc aggcgtggag ataactaacg cacacgggca gccaatcagc agcagcgtca
    11401 gccctttata aatccactcc tgccagctgg cggcaaacag cagcggtggc accagcgtca
    11461 ccagcagggc gacggccata atcgctggcg tataaatacg gctgaaacgg tcgataaacc
    11521 gctcaatggg ggcgcggcgc tcttcggctt cttcgatcag tttcagaatc cggtcaatgg
    11581 cgctggcacc cggttctgac agcacttcca gcgttaccag acggtctacg ctggtggctc
    11641 ctgcaggaac tttatcgccc gtcgcgcgct ccaccggaat ggattctccg gtcagggcgc
    11701 tttcatcaaa actggcaaac ggtgagagca gtttaccgtc ggcaggcaaa cgcccacctg
    11761 cggcgacttc aatcacattg ccaggccgca ggctgttaat cgccacctct tcccgctcac
    11821 cgttacgcag gcgcgtggcg gtttctggtt tcagcgccat taacgcgcta acgccctgac
    11881 gcgcgcggct ggcggcccag ccttccagtc gttcaccaat caaaaacagc agcaacacca
    11941 tcgcagcttc agccgttgcg ccaataaaca gtgcaccaat agcggctacg ctcattaagg
    12001 tttcaatggc gaagtagctg ccggatttga tcagccgtaa tgcctgacga gcaatcgggt
    12061 acagcccaac cagcgtggtc gcgataaacg ccagttgccc aaacggatga ttaaattgct
    12121 ccagacccca gctgattgcc atcatcacga ttagcgtaat cagcggcaga ttctctttca
    12181 ggcgcgatgc ttgtggctcg tcggcggcct gctcatcgcg cagggaatag cccgcttttt
    12241 gcaccgcaga ttcaacctgg gcacggatgt cattgtcggc atcgaccacc agtttttcgg
    12301 tggcgaacag tacctgcacc tgattcacac ctgcaagctg gcgcacggca ttttctacct
    12361 tgcgcgcaca ggcggcgcag tccatgccgc tgactttcca gctatagcgg gtgccggaga
    12421 cgttttcaga gagcgttggc gagctggaac atgcgccgtc gcagcaacag tcgttggcgt
    12481 tctgtaccgt ggttagcggt ttgaacgcag caaattgagg ggctttcttg ccgtgattgt
    12541 caggagtcga catggcatcc tccggttaag ttttttctca ttaaccgaag gatacactct
    12601 ggagtcgact ccagagtcaa gttttatcag agatacagcg agcggacgat gaggaaatga
    12661 ccggcaaagt agcaggccgc agcaatcgcg ttatctgccc ggaagcggcg gcgatagtgg
    12721 ctacccagcc agacaaagtt actgataaac agcaatgaag cgccaacaaa cgcagagagt
    12781 gccggagcgg tcggacggaa gaaccacagt tcacctgcca gccacaccat caccagcgtc
    12841 atgccgataa acgtgcagat aggccaacga tactcttcca gccgcgtcca gataatcgcc
    12901 agtaacagcg cgcccagaac cagcaacacc agtggcagag gccagaagaa agagagcgtc
    12961 atctgactgg cgaaatagat ggtgtacagc aggtgcgaga ggaaaaacgc gccgatggcg
    13021 tacatcagac gttgacgtgg caacagggtt agcgcatcgc ccagcagtga ggcgcacagc
    13081 cctgccagca ccagatagct aatggcgtcg aacatcggcg cttgccaggc cagtaacagc
    13141 aggagaagaa gggttaacgg tttaaacacc cagcgttgcc aggttggccc acgatacgat
    13201 gcatccacag atagccatgc ggaaagacag acagcgataa acgaccaaag catcttagtt
    13261 ccttgaattc gttatttttt cgtaagcgga atgcctacgg tcttgcttcc agtttagtgg
    13321 cgagggtgga cggatgacaa caccataact ggcagggtat gcttatttcc gcattttccg
    13381 atgagggata aagatgagca aaccacctct tttctttatt gttatcattg gcttaattgt
    13441 cgtcgccgca tcgtttcgtt ttatgcaaca gcgacgggaa aaagctgata atgatatggc
    13501 tccgctccag caaaagctgg tggtggtgag caacaagcgg gaaaaaccga ttaacgatcg
    13561 ccgttcgcgc cagcaggaag tgactccggc aggcaccagt atgcgctatg aggcaagctt
    13621 caaaccgcaa agcggaggaa tggagcagac gtttcgcctc gacgcccagc agtaccacgc
    13681 cctgacagtg ggcgataaag gtacgctgag ctataaagga acgcgctttg tcagctttgt
    13741 aggcgaacaa taaggttatt ttttaacttt aaatttcttc tgccagacca gcagttcaaa
    13801 cacgccgaaa aggaaaatcc gcaccttttc ggcggtggtc atctgcgggc catctttcgg
    13861 taaagtggat ttcaatagcg ccagttgcat accatgcatc agcacggtaa aaatcagcgc
    13921 cacgttaacg aaaatattca gcgggcgcgg gaagggatgc accagattga ggattaaaaa
    13981 tccccaaacg cagagcatta acaaacgacc aatattaatc agcatcactt tctccttgtg
    14041 cttcgcgttg atacagccga taagccacct gacccgccac tttttcccga tgtaatgacc
    14101 agtttgccgg aacagtgggc agaccgtttt cgacttcgct ttcgacataa atcagggctt
    14161 cgtcagccag ccagccgtta tcttccagta aatttatcgt ctcttctaac aagccacggc
    14221 ggaacggtgg atcgacaaac acgatattat gcggtgtgcc tttttgcgcc aggaatgaca
    14281 ttgcgttgct gttcaccacg cgtgcattgc ctgcttttag tgtcgccaga ttcttaatta
    14341 actgctgaga aactgcgcga tccatctcaa tcaacgtggc ccccgcagcg tagcgcgata
    14401 acgcttccag ccccagcgcg ccgctcccgg cgaagcaatc cagacattgg gcgtcaacaa
    14461 tgaccggagc cagccagtta aacaacgttt cgcgtacgcg gtcggtggtg gggcgcagac
    14521 ctgggctatc aggaaccggg agtttacggc ctcgccactg cccgccaata atgcggattt
    14581 ggccgctgcc ggaatgattc ggttttttca tgataaattg ctcaatccgc cagataacac
    14641 atacttgcag gcggtgatgt gaattaagtt aagtgataga ctatttcatc atttttttag
    14701 ctgctatgta catagcgtta acgctgtgcc atgaagcaac agcgaggagt gtagtcgcaa
    14761 atggcgaaag aaaaaaaacg tggctttttt tcctggctgg gctttggtca aaaagagcag
    14821 accccggaaa aagagacaga agttcagaat gaacaaccgg ttgtagaaga aatcgttcag
    14881 gcgcaagagc ctgtgaaggc ttctgaacaa gccgtcgaag agcagccgca ggcgcatact
    14941 gaagccgagg cggaaacttt tgctgccgac gttgtggaag tcactgaaca ggttgctgaa
    15001 agtgaaaaag cgcagcctga agcggaagtc gttgcacagc cggaaccggt cgtagaagaa
    15061 acgccggagc cagtggctat cgagcgtgaa gagctgccgt tgccggaaga cgtcaacgcc
    15121 gaagaggttt cgccagaaga gtggcaggct gaagcggaaa ccgtagagat tgtcgaagcg
    15181 gcggaagaag aagcggctaa agaagaaatc accgacgaag agctggaagc acaggcgctg
    15241 gctgccgaag cggcagaaga ggcggtgatg gtggttcctc cggtagaaga gcagccggtg
    15301 gaagaaatcg ctcaggagca ggaaaaaccg accaaagaag gtttcttcgc gcgcctgaaa
    15361 cgcagcctgt taaaaaccaa agaaaatctc ggttccggat ttatcagcct gttccgcggt
    15421 aaaaaaatcg acgatgatct gtttgaggag ctggaagaac agctgttgat cgccgatgtg
    15481 ggcgtggaaa ccacacgtaa aattatcacc aatctgacgg aaggcgcatc ccgcaagcag
    15541 cttcgtgatg ccgaggcgct ctatggcctg ctgaaagaag agatgggcga gattctggcg
    15601 aaagtcgatg agccgctgaa tgttgaaggt aaaacgccgt tcgtgatcct gatggtgggc
    15661 gtcaacggtg tgggtaaaac cacgacgatt ggtaagctgg cgcgtcagtt tgagcagcag
    15721 ggtaaatcgg tgatgctggc ggcgggcgat actttccgtg cagcagcggt tgaacagctt
    15781 caggtctggg gtcagcgcaa caatattccg gtgattgccc agcatactgg tgcggattcc
    15841 gcctctgtta tcttcgacgc cattcaggca gctaaagcgc gtaatatcga cgtcctgatt
    15901 gccgataccg ccggacgtct gcagaacaaa tcgcatctga tggaagagtt gaagaaaatc
    15961 gtccgcgtga tgaagaaact cgacgttgaa gcgccgcatg aagttatgct gactattgat
    16021 gccagcaccg ggcagaacgc ggtaagccag gccaaactgt tccacgaagc tgttggctta
    16081 accggcatca cgctaacgaa actggacggc acggcgaaag gcggggtaat tttctcggtg
    16141 gctgaccagt ttggtatccc tatccgctac attggtgtcg gcgaacgtat tgaggatttg
    16201 cgtccgttta aggcggacga ctttatagag gcactttttg cccgagagga ttaacaatga
    16261 ttcgctttga acatgtcagc aaggcttatc tcggtgggag acaggcgctg cagggcgtta
    16321 cgttccatat gcagccgggt gagatggcgt ttctgaccgg tcattccggc gcagggaaaa
    16381 gtaccctcct gaagctgatc tgtgggattg agcggcccag cgccgggaaa atctggttta
    16441 gcggccatga catcacgcgt ctgaaaaacc gtgaagttcc gtttctgcgc cgccagattg
    16501 gcatgatttt ccaggatcac catctactga tggaccgtac tgtctacgat aacgtggcga
    16561 tcccgctgat tatcgctggt gccagcggtg acgatattcg tcgccgggtg tcggcggcac
    16621 tggataaagt cgggctactg gacaaagcga agaacttccc tattcagctt tccggcggtg
    16681 aacaacagcg tgttggcatt gcccgcgcgg tggtgaataa gcccgcggta ctgctggcgg
    16741 acgaaccgac cggtaacctg gacgacgcgt tgtcggaagg gattctacgt ctgtttgaag
    16801 agtttaaccg cgttggggta accgtattga tggcaacgca cgacatcaac ctgatttcac
    16861 ggcgttccta tcgcatgctc accctgagcg atggtcactt gcatggaggc gtgggccatg
    16921 aataagcgcg atgcaatcaa tcatattcgg cagtttggtg ggcgtctgga tcgcttccgt
    16981 aaatcggtcg gcggctcagg cgacggcggt cgtaacgcac cgaaacgcgc gaaatcctcg
    17041 ccaaaaccgg taaatcgcaa aaccaacgtt ttcaacgaac aggtgcgcta tgccttccac
    17101 ggcgcattgc aggatctgaa aagcaaaccg ttcgccacgt ttttaacggt gatggttatc
    17161 gccatctccc tgacgctgcc cagcgtctgt tatatggtgt acaaaaacgt taatcaggcg
    17221 gcgacgcagt attatccgtc accgcaaatc actgtttact tgcaaaaaac gctggacgat
    17281 gacgccgctg cgggcgtggt ggcacagttg caggccgagc aaggcgtgga gaaagtgaac
    17341 tatctttctc gtgaagacgc actgggcgag ttccgtaact ggtctggttt tggtggtgcg
    17401 ctggatatgc tggaagaaaa cccgcttccg gcagtggcgg tggtgatccc gaaactcgat
    17461 tttcagggga cggaatcact caatacgctg cgtgatcgta tcacgcagat taacggcatt
    17521 gacgaagtgc ggatggatga cagctggttt gcccgtctgg cggcgttgac cgggctggtc
    17581 gggcgcgttt cggcgatgat cggcgtgttg atggtggcgg cagtgttcct cgtcatcggc
    17641 aacagcgtac gtctgagcat ctttgctcgc cgtgactcca tcaacgtgca gaaactgatt
    17701 ggtgcgacgg atggtttcat ccttcgcccg ttcctgtatg gtggcgcact gctgggattt
    17761 tctggcgcat tgttgtcatt aattttgtca gaaattctgg tgctgcgatt gtcatcggcg
    17821 gttgcggaag tggcacaggt tttcggaacg aagtttgata tcaatggttt atcgttcgat
    17881 gaatgcctgc tattgctgct ggtatgctcg atgattggct gggtggcagc gtggcttgcc
    17941 acggtacaac atttacgcca ctttacgcct gaataataaa agcgtgttat actctttccc
    18001 tgcaatgggt tccgtagcag ggaaagagat cccgttgtct cttcccggta tttcatctct
    18061 atgtcacatt ttgtgcgtaa tttattcaca agcttgcatt gaacttgtgg ataaaatcac
    18121 ggtctgataa aacagtgaat gataacctcg ttgctcttaa gctctggcac agttgttgct
    18181 accactgaag cgccagaaga tatcgattga gaggatttga atgactgaca aaatgcaaag
    18241 tttagcttta gccccagttg gcaacctgga ttcctacatc cgggcagcta acgcgtggcc
    18301 gatgttgtcg gctgacgagg agcgggcgct ggctgaaaag ctgcattacc atggcgatct
    18361 ggaagcagct aaaacgctga tcctgtctca cctgcggttt gttgttcata ttgctcgtaa
    18421 ttatgcgggc tatggcctgc cacaggcgga tttgattcag gaaggtaaca tcggcctgat
    18481 gaaagcagtg cgccgtttca acccggaagt gggtgtgcgc ctggtctcct tcgccgttca
    18541 ctggatcaaa gcagagatcc acgaatacgt tctgcgtaac tggcgtatcg tcaaagttgc
    18601 gaccaccaaa gcgcagcgca aactgttctt caacctgcgt aaaaccaagc agcgtctggg
    18661 ctggtttaac caggatgaag tcgaaatggt ggcccgtgaa ctgggcgtaa ccagcaaaga
    18721 cgttcgtgag atggaatcac gtatggcggc acaggacatg acctttgacc tgtcttccga
    18781 cgacgattcc gacagccagc cgatggcacc ggtgctctat ctgcaggata aatcatctaa
    18841 ctttgccgac ggcatcgaag atgataactg ggaagagcag gcggcaaacc gtctgaccga
    18901 cgcgatgcag ggtctggacg aacgcagcca ggacatcatc cgtgcgcgct ggctggacga
    18961 agacaacaag tccacgttgc aggaactggc tgaccgttac ggtgtttccg ccgagcgtgt
    19021 gcgccagctg gaaaagaacg cgatgaaaaa acttcgcgct gccattgaag cgtaatttct
    19081 actactaagc agagaaccct ggatgagaat ccggggtttt tgttttttgg gcctctgcaa
    19141 taatcagttt ccccctccgg caaaacgcca atccccacgc agattgttaa taaactgtca
    19201 aaatagctat tccaatatca taaaaatcgg gtatgtttta gcagagtatg ctgctaaagc
    19261 acgggtagtc atgcataaaa cgaaataaag tgctgaacaa caacatcaca acacacgtaa
    19321 taaccagaag aatggggaac ctcaggatga acatgaaggg taaagcgtta ctggcaggat
    19381 gtatcgcgct ggcattcagc aatatggctc tggcagaaga tattaaagtc gcggtcgtgg
    19441 gcgcaatgtc cggtccggtg gcccagtacg gtgaccagga gtttactggc gcagagcagg
    19501 cggttgcgga tatcaacgct aaaggcggca ttaaaggcaa caaactgcaa atcgtgaaat
    19561 atgacgatgc ctgtgaccca aaacaggcgg ttgcggtggc gaacaaagtc gttaacgacg
    19621 gcattaaata tgtgattggt cacctctgtt cttcatcaac gcagccagcg tcagacatct
    19681 atgaagatga aggcatttta atgatcaccc cagcggcaac cgcaccagag ctgaccgctc
    19741 gtggctatca gctgatcctg cgcaccaccg gcctggactc cgaccagggg ccgacggcgg
    19801 cgaaatatat tcttgagaag gtgaaaccgc agcgtattgc tatcgtccac gacaaacagc
    19861 agtacggcga aggtctggcg cgtgcggtcc aggacggcct gaagaaaggc aatgctaacg
    19921 tggtgttctt tgatggcatc accgccgggg aaaaagattt ctctacgctg gtggcgcgtc
    19981 tgaaaaaaga gaatatcgac ttcgtttact acggcggtta tcacccggaa atggggcaga
    20041 tcctgcgtca ggcacgcgcg gcagggctga aaactcagtt tatggggccg gaaggtgtgg
    20101 ctaacgtttc gctgtctaac attgcgggcg aatcagcgga agggctgctg gtgaccaagc
    20161 cgaagaacta cgatcaggtt ccggcgaaca aacccattgt tgacgcgatc aaagcgaaaa
    20221 aacaggaccc aagcggcgcg ttcgtctgga ccacctacgc cgcgctgcaa tctttgcagg
    20281 cgggcctgaa tcagtctgac gatccggctg aaatcgccaa atacctgaaa gcgaactcgg
    20341 ttgataccgt gatggggccg ctgacgtggg atgagaaagg cgatctgaaa ggctttgagt
    20401 tcggcgtatt tgactggcac gccaacggca cggcaaccga tgcgaagtaa cagatagata
    20461 aatgaaaatg cccgatgcgc tgaactgcca tcgggttttt ttgtgtttgt cgaaatggtt
    20521 gtagaaagat taaacaatcc aggatatgct taatgtacgg tgacataccg tacatattat
    20581 acggagcagg agagttcaca tgtctgctgt taaaaagcag cgtatcgatc tgcgtttaac
    20641 tgacgatgac aaaagtatga ttgaggaagc ggcagcgata tctaatcagt ccgtcagcca
    20701 atttatgttg aacagcgctt cgcagcgggc tgcggaagtg attgaacagc atcggcgggt
    20761 gatcctcaat gaggaatcct ggacgcgggt gatggatgcg ctgagtaatc caccgtcacc
    20821 tggtgaaaag ctaaaacgtg cggcaaaacg tcttcaggga atgtaataat ttatggatga
    20881 tctgacgata gagattctga ccgatgatgc agattatgat ctacagcgat tcgactgcgg
    20941 cgaggaagcg ttaaatctct ttctgacgac acatctcgtt cgtcaacatc gcaacaaaat
    21001 tctgcgagcg tatatccttt gtcgcaacac tccagaacgt caggtgctgg gatattacac
    21061 attatgcggc agttgttttg aacgagccgc attgccctcg aaatcgaaac agaaaaaaat
    21121 tccctacaaa aatattccca gcgttactct tgggcgtctg gcaattgatc gttcattaca
    21181 ggggcaggga tggggagcaa cactggttgc tcatgccatg aaagtcgtct ggtcagcctc
    21241 tttagcggta ggtattcacg gtctttttgt cgaggcgctg aataaaaaag cccatacgtt
    21301 ttatcaatcg ctgggcttta tcccgttggt cggagaaaac gaaaatgcgt tatttttccc
    21361 aaccaaatcc atcgaactgc ttttcacaca gagcgattaa cgcttctccc agccgccctg
    21421 ttgcgccgta aatcccagtg cctgcataaa cgccgtcatc acaccgcgat cttccacgcc
    21481 tgcatccgcc atccaccagc atgaaacgcc aggattgtta cgcaaaacct cttccagcag
    21541 atattgcccc acaccgcgac ggcgggtgac ttcccgcacg cacagggaat ccagtgctcc
    21601 ctcggtgccg cttaaggtca cccgcacggc cgctagcagg cgctcgttaa aacgcgcggc
    21661 gtagatacgg tggttatcgt caacctgtaa agaggaaggg gaatactccg gccagatctt
    21721 ttgcaggtca atccggtcct ggtcgctaaa gttttctaat cgaatgatgg tcagcttcat
    21781 ggggaacccg tgtaaatcac aaaagtggaa ccagtgtagc gaaataattt aatcggaggc
    21841 tttctctttt ttatttcttt tggcaggtga ttaatttttt aa
//
LOCUS       DAJOWB010000077        21740 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJOWB010000077 DAJOWB010000000
VERSION     DAJOWB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.077"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..1861)
                     /locus_tag="OHO85_003109"
     CDS             complement(<1..1861)
                     /locus_tag="OHO85_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCQ2672452.1"
                     /translation="MNNSRLFRLSRIIIALTVASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQ"
     gene            complement(2134..3063)
                     /gene="alsK"
                     /locus_tag="OHO85_003110"
     CDS             complement(2134..3063)
                     /gene="alsK"
                     /locus_tag="OHO85_003110"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HCQ2672453.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPG
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMIQKYLRRPLPHQVV
                     RFIAASSSDFNGAQGAAILAHQHFLPQSCAKAP"
     gene            complement(3047..3742)
                     /gene="alsE"
                     /locus_tag="OHO85_003111"
     CDS             complement(3047..3742)
                     /gene="alsE"
                     /locus_tag="OHO85_003111"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HCQ2672454.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLATKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RCHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTTQIL
                     AAKSEVQPHAKTA"
     gene            complement(3753..4733)
                     /gene="alsC"
                     /locus_tag="OHO85_003112"
     CDS             complement(3753..4733)
                     /gene="alsC"
                     /locus_tag="OHO85_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HCQ2672455.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGV
                     DPFLAAMIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            complement(4712..6244)
                     /gene="alsA"
                     /locus_tag="OHO85_003113"
     CDS             complement(4712..6244)
                     /gene="alsA"
                     /locus_tag="OHO85_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HCQ2672456.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNISYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLVHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCCPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            complement(6371..7306)
                     /gene="alsB"
                     /locus_tag="OHO85_003114"
     CDS             complement(6371..7306)
                     /gene="alsB"
                     /locus_tag="OHO85_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HCQ2672457.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEAFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKILVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVNAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            complement(7365..8255)
                     /gene="alsR"
                     /locus_tag="OHO85_003115"
                     /gene_synonym="rpiR"
     CDS             complement(7365..8255)
                     /gene="alsR"
                     /locus_tag="OHO85_003115"
                     /gene_synonym="rpiR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="als operon DNA-binding transcriptional repressor
                     AlsR"
                     /protein_id="HCQ2672458.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            8614..9063
                     /gene="rpiB"
                     /locus_tag="OHO85_003116"
     CDS             8614..9063
                     /gene="rpiB"
                     /locus_tag="OHO85_003116"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HCQ2672459.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEVDGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            9132..9461
                     /gene="yjdP"
                     /locus_tag="OHO85_003117"
     CDS             9132..9461
                     /gene="yjdP"
                     /locus_tag="OHO85_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCQ2672460.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(9608..10366)
                     /gene="phnP"
                     /locus_tag="OHO85_003118"
     CDS             complement(9608..10366)
                     /gene="phnP"
                     /locus_tag="OHO85_003118"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCQ2672461.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(10368..10802)
                     /gene="phnO"
                     /locus_tag="OHO85_003119"
     CDS             complement(10368..10802)
                     /gene="phnO"
                     /locus_tag="OHO85_003119"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCQ2672462.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHNAHRFYLREGYEQSHFRFTKAL"
     gene            complement(10789..11346)
                     /gene="phnN"
                     /locus_tag="OHO85_003120"
     CDS             complement(10789..11346)
                     /gene="phnN"
                     /locus_tag="OHO85_003120"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCQ2672463.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(11346..12482)
                     /gene="phnM"
                     /locus_tag="OHO85_003121"
     CDS             complement(11346..12482)
                     /gene="phnM"
                     /locus_tag="OHO85_003121"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCQ2672464.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            complement(12479..13159)
                     /gene="phnL"
                     /locus_tag="OHO85_003122"
     CDS             complement(12479..13159)
                     /gene="phnL"
                     /locus_tag="OHO85_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCQ2672465.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            complement(13336..14094)
                     /gene="phnK"
                     /locus_tag="OHO85_003123"
     CDS             complement(13336..14094)
                     /gene="phnK"
                     /locus_tag="OHO85_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCQ2672466.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSKADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(14091..14936)
                     /gene="phnJ"
                     /locus_tag="OHO85_003124"
     CDS             complement(14091..14936)
                     /gene="phnJ"
                     /locus_tag="OHO85_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCQ2672467.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(14929..15993)
                     /gene="phnI"
                     /locus_tag="OHO85_003125"
     CDS             complement(14929..15993)
                     /gene="phnI"
                     /locus_tag="OHO85_003125"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCQ2672468.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSIAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            complement(15993..16577)
                     /gene="phnH"
                     /locus_tag="OHO85_003126"
     CDS             complement(15993..16577)
                     /gene="phnH"
                     /locus_tag="OHO85_003126"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCQ2672469.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(16574..17026)
                     /gene="phnG"
                     /locus_tag="OHO85_003127"
     CDS             complement(16574..17026)
                     /gene="phnG"
                     /locus_tag="OHO85_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCQ2672470.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(17027..17752)
                     /gene="phnF"
                     /locus_tag="OHO85_003128"
     CDS             complement(17027..17752)
                     /gene="phnF"
                     /locus_tag="OHO85_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCQ2672471.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(17773..18552)
                     /gene="phnE"
                     /locus_tag="OHO85_003129"
     CDS             complement(17773..18552)
                     /gene="phnE"
                     /locus_tag="OHO85_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCQ2672472.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(18658..19674)
                     /gene="phnD"
                     /locus_tag="OHO85_003130"
     CDS             complement(18658..19674)
                     /gene="phnD"
                     /locus_tag="OHO85_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2672473.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(19699..20487)
                     /gene="phnC"
                     /locus_tag="OHO85_003131"
     CDS             complement(19699..20487)
                     /gene="phnC"
                     /locus_tag="OHO85_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCQ2672474.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(20620..21063)
                     /gene="yjdN"
                     /locus_tag="OHO85_003132"
     CDS             complement(20620..21063)
                     /gene="yjdN"
                     /locus_tag="OHO85_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCQ2672475.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(21223..21558)
                     /locus_tag="OHO85_003133"
     CDS             complement(21223..21558)
                     /locus_tag="OHO85_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCQ2672476.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         4705 a         5818 c         6062 g         5155 t
ORIGIN      
        1 cttgcccggt cagcacggcg tgcagatctt cacggctgat gtagaaagag gcgtcagctt
       61 gggattgcag tgttttacgg tagttaagca cgctatcgtt cagcgtcagg ttgaggttat
      121 cgccattgct catattgaag ttcaggctga tatttttacc cgcggctttc gcgctatcga
      181 ggcgaacgga cataaagtcg aacagcattt cgaccgacat cccgcgaatg gtgtccgggg
      241 aaccggtggt gccgtggctg aacttatgca ccccttcgcg cagctctttc gcgccggtca
      301 ggtagaaacc gcgccaggtg gcggactcgg cctgatagcc cagctgttca aagttattcg
      361 cttgcagatt cttcgcgacc tggtcacccg ggttggcggc aatcacctgt ttcagcagtt
      421 ctgccgacca gcggtaatca ccttgcttgt tcgcttcttg cgccaggttg atgacacggg
      481 cagaaccgcc cagcgcctgc acgtaacgtt tacccatctc cacctgacca tacggatgca
      541 ggttagccgg gttaccgtcg taatagccaa gatagaagtt atacaccgcg cgggcgttgt
      601 ggctgacaga accgtaatag ccacggctgg cccagttatt ggcaagtgca ggcggcagtt
      661 taatcatgtc gccgatttca ttcatggtgt agccctggtt cgccaggtgc agggtctggt
      721 cgtgaatgta cttgatagta tcgcgatatt taccaatata atcattgata tgcttattgc
      781 cccagaccgg ccaggtgtgc ggcataaaca ggacttccgc gtcgttaccc cacatatcca
      841 gcgtttcgtt cagatactcg gtccacttgc tggtgtcgcg ggttttcgcg ccgcgtagag
      901 tgtagaagtt gtgcagggta tgcgtggcgt tctcggcggt acacagcgct ttcagggccg
      961 gaatatagaa gtgcatttcg gctggtgctt cgctacctgg ggtcatcagg aagtcaaact
     1021 ccaggccgtc gataatcatc ttctcaccgg ttttggtgat ggttttgttc ggtgcgatga
     1081 tgctcgggta gcccgatgcc agcgttacgc caagaccatt gccgatgtta ccctgagggt
     1141 tatgcgccag cagcaggccg taggagtaaa gtgcacggcg gctcataata ttacccgcca
     1201 gtacgttttc gctgatggct tcgtccataa agccagcagg ggcgataacc tgcactttac
     1261 cggatttcac atcggcttca gagataatgc ctttcacgcc gccatagtgg tcggtatggc
     1321 tgtgggtata aataaccgca acaatcggtt tttgcgggcg attttggaag taaaggtcaa
     1381 gggcggcttt tgcgctcggt ggcgtcacca gcgggtcgat gacaataatc ccggtatcac
     1441 cttcgataaa ggtgatgttg gagatatcct gaccgcgaac ctggtacatt ctgtcggtga
     1501 ctttaaacag gccggaaata ccgtttaact gcgactgacg ccacaggcta gggttaacgg
     1561 tttcgggcgc gggcgcattg atatcaaatt tataatcttc cccgcgataa tatggtttgc
     1621 cattagcatc gcgcagaata ccttcatcca gcagcggagc gataaatcca cgctgagcgt
     1681 cgtcaaaatc ctgacgatcg ctaaacggta atgatttggc gtaattctga ttaacctgtt
     1741 gggtatattg agtggcggct ttcgcttcat ctgttgcgta agcggtattt accatcatgc
     1801 cgctggcgac agttaacgca ataataattc tgctcaaacg gaataaccga gagttattca
     1861 tataaatctc catttatcat ttatgtcgta aatatgtaat ccatgcaaaa tacgtcattt
     1921 aaagctgggg ttatcataat tgggaatttg cgactttaat aagtggaagt gtgagctgaa
     1981 cgatccattt tattaggcat ttgtgctgaa gaggaaaagt tacagtaaaa ctatcattgc
     2041 agaaaataat atgagccgtt ttatgtgttt attatatata ttgtacctgt cgttggcaca
     2101 gcgggacatt gcgctactgt gccaacgctt tactcatggg gctttagcac aggactgtgg
     2161 caaaaaatgt tgatgcgcca atattgctgc accctgagcg ccattaaagt cagaagatga
     2221 ggcggcaata aagcgcacga cctgatgcgg cagtggacgg cgcaggtact tttggatcat
     2281 ggcaatgaga gtctcgcgtg ggaaggcggg catatccatc acgccaccgc ccagaatcac
     2341 cgcatcggga tcgaacagat taatgctggt ggcaatggcc cgtgccgcgt tttcaagcag
     2401 actctggacg aaaggggcgt tttccgcatg gacgaaaagg tcgctcaatg ggtaatttcg
     2461 gggctgttgt tcgtaccagc gtcttagcgc cattccagag caattggttt ccaggcaccc
     2521 aggattgcca cacgcgcagt gttgggtcat atctcccagg gggatatgac ccagttcgcc
     2581 tgccacaccg tgtgcacccg tccacggcgc accgttcatc cacactgcga accccatccc
     2641 cgtaccgaga taggccgcca gaaccagttg ttgcgtaagg cggttttcta ctacgtccca
     2701 ggagagttgc aggttaacgt cgcgggaaaa ctcaaccgga caattcagcg tattttcgag
     2761 cttatcggcg agatcatata aatccgccgc tgttaacggc aggttaggcg tagaaattat
     2821 ggtgcgttta tctttactga ccagcgccgg aaatcccatc accagaccat gacagcgagc
     2881 gttaaagcgc ctgagttgct cgtcaatcat ttcgccgata cccgacacca ggccgggagc
     2941 aatgacttct gcggtccgct ttttttcgca gtgtagcgtt tcaccttctg ctgtccgcag
     3001 acaaaagcgg atatgcgttg cccccatatc cacgcccgct acgacgttat gctgtttttg
     3061 catgaggctg tacctcgctt tttgcagcca gaatctgcgt ggtcataatt ctccatgctt
     3121 cgtcgatatt ttccgcatga ttaaacaggc cggaagtgcc gacgataaag acatccgccc
     3181 ctgccgccat cagtttttcg taagttgcct ggttgcagga accgtccacc tcaatttcgt
     3241 actccagacc ttctcgttca cgccatgcct tcagttcggc aagtttatcc agcatttcag
     3301 gaatgaacgg ttgtccggca aagccgggat cgacagtcat gaccgtaatt ttatcggcct
     3361 tatggatata gtatttcatg gcctcaactg gtgtctccgg gttaaggatc agccccactt
     3421 tcatgtcatg acagcggatt tcatcaatca ggcggaacgc ctgaccgttg atagtttccg
     3481 gatgcaaagt gatgaaatca gctcccgcac gcgccagttg agcaatgtaa tcctgcggcc
     3541 gcgtcaccat cagatgacag tcgagcggtt tagttgccag ttttttaacc tgacttacga
     3601 agaacggtga gagtgtcaga ttggggacaa agtgaccatc catgatatcg atgtgaaagt
     3661 aatcggcatg gctgtcgata aattcgatct gttctttaaa tttcagcaga tccatacaca
     3721 ttaacgaggg ggagattttc atgttcaatt ccttacttac tgataagacg gtcaagggcg
     3781 acagccgcga taattaatcc gcccatcacc accagttggt aataggtttg tacctgcaaa
     3841 atattcagac cgttgttgat ggtgccgatg atcaacccgc caatcaccac agagaaaatg
     3901 cgccccttgc cgccgaagaa actggtgccg ccaatgatgg cgctggcaat ggcataggtt
     3961 tcaaaaccca taccggcaag cggttctgcg gcaccgagtc gtgcagttga gacgacgcct
     4021 gccagacctg cacaaacacc ggagatgata aacaccacca ggatgtggaa tttcacgtca
     4081 atcccggaat agaacgccga atttttgttg ccgcccagtg cgtagatgtt gcgcccgagc
     4141 cgcatacgcg ttgtcagaaa ccaaaggatg agcgcgacaa ttagcgagaa gataacgggg
     4201 acaggtatcc caattacgct ggcggcaaag aagttcacga agtcaaagga gaagccgtat
     4261 accgagttgg catcggagat caccagcgtg atcccacgga aaatcgcgtt ggtgccaagg
     4321 gtgatgatga acgggtgtag ccccgtccag ttgaccaggc agccgttgat cgcccccagt
     4381 gcgccgccaa ccagtacacc gccaatcatc gctgcgagaa acgggtcaac acctgccaac
     4441 atcagtttgg cggtcaccat accggaaagc gccagaatcg cgccaaccga gaggtcgata
     4501 ccagcgacca ggatagcgaa aaactcgccc atgccgatca ataccgtcac ggagctttga
     4561 acaaaaatct gggtaatatt attggtggtc agaaaatatt ctggcgacag cgaaccaaaa
     4621 atggcgacga tgatcgccag gataaaaaag gtgccgtatt tatcccagaa cagcgcaaag
     4681 ttgaacggtt tcttctcgct cgcttcgctt tttactcttg tggtaaagcc catgccataa
     4741 tctcctcttc gctcatgtca tcgcgattcg tcaggatttg cgtcagtcgt ccttcgcaga
     4801 acacggcgat gcggtcgcag acggtgataa tttcaggtag ttcagatgac accatcagga
     4861 tgacttttcc gtcgtccgcc agttggcgca tcactttgta aatttcggct ttcgcgccaa
     4921 cgtcgatgcc gcgggtaggt tcatcgaaaa taatcacttc cgggcaacag cacagccatt
     4981 tggagatcag gactttctgc tgatttcccc cggagagttc ggtgatattc tggtttaccg
     5041 aatgacattt cagcgccagc agttcgcgtt gattttcagc ggtacgttgc tcgtcaactt
     5101 catgaaacaa gcccatcgcg cctttatagc cgccgtcttt cagactgcgg ctgatcgcca
     5161 tgttctgagc gatggaaaag ttgggaaaaa aaccgttatc ccggcggctt tcagtgatgt
     5221 aagccatccc ttttttcact gcatccaggg gggaacgtgg agagatatct ttgccattaa
     5281 gacggatttc tccgccagcg cgtttatcaa cgccaaacag acaattcatc agttcagtac
     5341 gtccggaacc gaccagcccg gcaaagccta atatttctcc ccggcagacg ctaaatgaga
     5401 tatcccggac cttttttctg tcacggctgg tgacgttgcg cacctcaaaa accgtatcgt
     5461 gcacaaggtt gctgacattc tccttcatcg cgttaaaacg gttttgcagt tcgcggccta
     5521 ccatcagacg gacgatatcg tcatttgaca catcgcttac catgccgctg caaacgctgc
     5581 tgccgtcttt catcaccgta tagcggtcgc aaatacggcg aatttccgcc aacttatgcg
     5641 agatatagac gatggccgta ccctctttac gcaactgatt catgatcaga aacagatagt
     5701 ccacctcttt attggtgagt gaggaggtgg gttcatccat gatgatgact ttggcatcga
     5761 gcatcagcgt tttggcaatt tccagcatct gcttgtggct gatagataga ttcgccactt
     5821 tctcatctaa atcaactttc aagcccacgc gtaataacat catggcggcg cggacacgca
     5881 tttctcgcca gtcgataata ttgacgccac agattttttt cgtcagatga cgaccaatat
     5941 ataaattttc cagtacggtt aattcatcaa taacgctgag ttcctgataa ataatcccga
     6001 taccgagttg tgccgctaat ttatgatcca gcttgttata gctaatgtta ttaatggtaa
     6061 tggtgccttt ggtcggctca tgtattccgg ataaaacctt cattagcgtg gatttacccg
     6121 cgccattttc tcctagtaat gcatgtattt caccaggata aaccgttaaa ttaaccgact
     6181 ttaatgcgtg aaccggacca aaggacttgc cgatccccgc catcgatata tatggcgtgg
     6241 ccataaataa cctctctgca cgaaatatcg tattattgcc tgatggctac atttatcagg
     6301 tctacgttca ggttattttg ttggccggat aagacacttc attagcatca tcatccggca
     6361 tcattcagtt ttattgagtg accaggattg aatcgaccag tttaaattcc ggtgctttat
     6421 ccagcgggat aaccttgccg gatttctcag cgttaaccat cagcttcaga cccgttgcgc
     6481 caatatctgc cgggttctgc gcaaccgtcg cggtcatttg tccggcttcc accattttgc
     6541 gggcttccgg aattccatct gtaccgacga ccaatatttt gcccgttttt ccggcgtttg
     6601 cgactgcctg agcaacaccc attgccatcg tgtcattcgc gcaatagatc gctttaatat
     6661 tcggattacg ttgcaacacg ttagtggcga catccagtgc tttaatgcgg tcccagtcag
     6721 cgggctggct ggcgacaagc ttgatctggc ttgctttttt gaaggcttcg gtggcaccat
     6781 tacgacgcgc ttcaccggag gcgttaccgg ctttaccctc aatgattgcg acttcaccac
     6841 cttcagcgcc caatttgtca ataatgaacg acgcgccttt cgccccgaca gcaacgttat
     6901 cggtggtgac aaaagcttcc acattgccgc cagctttttt cagattatcc atgtcgattt
     6961 tttcatcgag attaaccaga taaatgcctt ttttccatgc gcgggcgaca ggcatgacca
     7021 gattcactga ggataatgga gcgaaggcga tacctttgta atttttatta ctgagatctt
     7081 caaataactg caattgagat tgaaaatcgc cttctgaagg agaggcaaaa atatcaacgc
     7141 tgacgcccag tgtttttgct tcatcttcaa tgcctttttt catatctacc caaaatgggt
     7201 tggagagggt tttcaatacg acagcatatt cggcggcagc aaaagcgctg gttgacaaca
     7261 ttaagcccac gagtgtgccg ctgaaatatt tcagatattt attcataata atgttctcac
     7321 ggtaagggga caggtaactc cgcgatggcg gaattacctt tttcttattt cagcgcgcct
     7381 ggtgagaaga aatcaacaat tgcgccggtt ttttgcatat taatattagc ttgttcaatg
     7441 ttgagctggg cgacagagac aaaaaaagcg tccagcaaag ttagttgtaa tattcttgcc
     7501 gaggcattac gacctaataa cggcgtttcc ggggctggtg agcaaataat ataatcggcc
     7561 agtttcgcta tcggtgaatg gtagctatgg gttatacaaa taatctttgc cccgttcttt
     7621 tttgccagtt ctacggccgc ttttacatca ctggttcgcc cggaatgggt cactaccagc
     7681 acaacatctc cttcctgtaa caacgaagcg gacatcatca tgatgtgagc atcaggatag
     7741 gcctgacagc gtacgccaat gcgcaagaac ttgtgctgta catcagcaca gatagcattt
     7801 gatcctccgg caccgtacaa atcccgctgt ctggcctgat agaaaaagcg ggcggcacgg
     7861 tggatctcat caacgttgac gatcgactga ccttccataa tggtgcgtaa agtgatgtta
     7921 aataccttat tcaccacatc ctgcggcgct tcatcaaaag ccaactcgga aggcaatacc
     7981 tgttctgact gagaaaaata atcttccagc gcactgcgta agttacgaaa gccgctaaac
     8041 cccagcagct ttgatacctt aactatcatc gcttcagata ccgccagagc ttctgcgaca
     8101 tctttaattg cgggtgcaca actcaggtta ccgggtttga gtaaccactc tacgatgcgg
     8161 ctttcatttt ctgtcattcc ttcctgcttc attcgcaggt aaggcgctaa ccctataccg
     8221 ttcggaagcg ctgaatcaaa ctctgactgg ctcatcacgt tctcttgtcc ttgaattgaa
     8281 gacgccacta tatcaccaca gcaagaacag aaaagatgtt ggcgacttca caaaaaaata
     8341 aaatttataa agtttatttt attgtgaaca atggcgagtg gcttaaaatt ttatttcatt
     8401 taattagttt aaaaacatga tgttatttgt ttttctggtt ttttagatga tagatgttgc
     8461 tggatggtga gtgtctttaa cgctattgcg ctgtcagatg taactctgta aaacagatca
     8521 ggaaggcgta attcatcgat tttgggtgtt tttaattcat taacatcaca aatgtttttt
     8581 gattgtgaag ttttgcacgg acggggaaga tgaatgaaaa agattgcatt tggctgtgat
     8641 catgtcggtt tcattttaaa acatgaaata gtggcacatt tagttgagcg tggcgttgaa
     8701 gtgattgata aaggaacctg gtcgtcagag cgtactgatt atccacatta cgccagtcaa
     8761 gtcgcactgg ctgttgctgg cggagaggtt gatggcggga ttttgatttg tggtactggc
     8821 gtcggtattt cgatagcggc gaacaagttt gccggaattc gcgcggtcgt ctgtagcgaa
     8881 ccttattccg cgcaactttc gcggcagcat aacgacacca acgtgctggc ttttggttca
     8941 cgagtggttg gcctcgaact ggcaaaaatg attgtggatg cgtggctggg cgcacagtac
     9001 gaaggcggtc gtcatcaaca acgcgtggag gcgattacgg caatagagca gcggagaaat
     9061 tgagattcat ccactacttg catggatgag taatgactaa tgtggataga gtttcttttt
     9121 gaggttggct aatgaaacgc tttccgctat ttcttttatt caccctgctc acgctgtcca
     9181 ccgttccggc ccaggctgat attatcgacg atactattgg taatattcag caggcgatta
     9241 atgacgctta taaccctgac cgtgggcgag actatgaaga ttcgcgcgac gacggctggc
     9301 agcgtgaagt gagcgacgat cggcgcagac aatatgacga ccgccgccgc cagtttgaag
     9361 atcgccgcag gcagctggac gatcgccagc gccagcttga tcaggagcgt cggcaactgg
     9421 aggatgaaga gcggagaatg gaagatgagt atgggcgatg aggttgggta tggagcaggc
     9481 atcgcccctc accccaatgg ggagagggag aaaacgagcg caatattcaa tattgagcac
     9541 aatcggtccc ctcgcccctc tggggagagg gttagggtga gggggccagc gcgcacattc
     9601 cctctcatca cgccacccca atctccatcc catcaaaccc cacctcaaac cctgacggta
     9661 gtgcgttttc catcagccac gcatcaaact ggtggctgat atgggtcaga atcacctgtg
     9721 gcgagcggat aacctgattc agcgcaagca cggtatttaa atcacagtga ttacgcggtg
     9781 catccgcgcg cggcgggtga ctgcaatcaa tcaccatcac ctgcggatga ttattgagta
     9841 aaaatttcag cgttttttcc ggcaggccag cggtgtcaga cagccacgcc acccggctat
     9901 gtgccgtttc cagcagataa ccgaaggtca gttttgagtg gttaagcggc agcggcgtga
     9961 cctgcaaccc ctgcaaatca aacaccacaa acggttccac tgtgtggctg aaatcaagca
    10021 ggcccggatg tttaaacaga tcgtcgcagc cctgttcatc cggcgggccg tagaccggga
    10081 tcacatcgcc cacgccccag cgtagcggaa atagcccctg gacgtgatcc atatggtagt
    10141 gcgtcagcaa aaactgctgg aacgatccag gcgaccagcg atcggtgaga tcgtgccgcc
    10201 cggcgtcgat cagggtgatc gcgtcgttaa acttcactac gccgctgcat ggctggcggc
    10261 gatactgcgg cgagcgccgc gctctggcgc aggccgcgca ctcgcagccc catgccggaa
    10321 cgccctgtgc gccgccagtg ccggtgagcg tgagggtcag gctcatgtta cagggccttg
    10381 gtgaagcgga agtggctctg ctcgtagcct tcacgcaggt aaaaacggtg tgcgttgtgg
    10441 cgcttcacgt tggtggaaag ctcggtcatt tcggccccgg cctggcgggc ttcttcttct
    10501 gcccatgcca gtaatttact gccgacgttc agaccgcgcg cctgcggcat caccaccagt
    10561 tcttgaattt cgccgatcca gttgacatga tgcagatgaa actgcaaatg caggccgatc
    10621 atgccgacaa cttcgccatc cagcagcgcc agatggtagc gcatgtttgg gtcgcgcagg
    10681 ttggcgttaa agcccacgcg aaacgcatgg tggtcaaact ccgcctgttt tagctcacaa
    10741 atcagcgcgt aaaccgcgtc ggtatcgtac tgcgtggccg ggcgaagctc acaagcaggc
    10801 atggtgtttc tccttctgat ggatcagcgt cagcagcgtg tcgaccgact ggcgcaggct
    10861 gccgtcattg ttgagcgtat ggcaatcctg tggagtatag cgggcggcgc gcgccaggcg
    10921 ggcgttaatt tcactggcgt tttcacggcc acggttttcc aggcgctggc gcaggatctc
    10981 cggcgaaacc tgtaaacaga cgggcagcag cgccgattga tagcgcgccc gtgcctgcgg
    11041 caaatgcgcg cgtgaaccgt tgacgactac gtcgaatcca gcgtgcagcc agaggtcaat
    11101 ctcgatgccg acgccataat acagaccgtt agcgtgccag cttaaggcca gcaggttttg
    11161 ccccgcgcgg gtaaaaaact cccgctcact cagggcgata tggttttcac ttccggcgct
    11221 ggcgtcgcgc gtgatgtagc gatgcgccac cagtaactgc gtttgttccc gcaggcggag
    11281 ttccgccagc aggctgtctt tcccggagcc ggacggcccc attaaccaaa tcagttttcc
    11341 catcatcaga acaccctttt accctgacgc cagacgtggt cgatatgaat gtgattgccc
    11401 tggcgatgcg ccagcaccag gtcagcgcgt ttaccctcgc caatcacccc gcgatcctga
    11461 agattcagcg cctgcgccgg atttttggtc accagcctca ccgcctgcgg tagcgtaaag
    11521 cggttgctct cgtcatcggc gacgcggaac gccgcatcca gcaggctggc ggggtagtag
    11581 tcggaagaga ggatatccag cagaccaagc tgcgccagtt cactggccgc cacattgccg
    11641 gagtgcgagc cgccacgcac gatatttggc gcacccatca gcacgttcat gccatgctta
    11701 cgcgaggctt ccgccgcttc gaacgtggtg ggaaattcgg cgatcacgct gccaagttgg
    11761 tgggattcgg caacgtgggc gtgcgtggcg tcatcgtggc tggcgagcgc aatttgtcgc
    11821 gcgcggcaca gggcggcgat ggattcgcga ttcggctgcg accagcgtgc ggcgagcgcc
    11881 aattgctctt cttcgtactg ctgcatctgc gcatcggtga gggagtattt gccctgataa
    11941 tattcgcgat acttctcgcg gttagcgaac tggcgctggc ccggtgagtg gtccatcagc
    12001 gacaccagcg tcaccggttc gcgctgaacc agtttttcaa acagcggcag tgtggtgtga
    12061 tgcggcagtt cgcagcgcag gtgcaggcgg tgctcggcgc ggttgacgcc gcgtttttgc
    12121 gtctcttcga tggcgttgat catcttctcc agattctcca gccgatcgcc gccgtcgcgc
    12181 acatcaccaa tcgccacggc gtccagtacg gtggtgatgc cgcttgccac catcagcgcg
    12241 tcgtggctgc tcatcgccga atgggcgggc cagtcgactt tcgggcgcgg ggtgaagaat
    12301 ttatcgaggt tgtcggtatg cagctcaatc aggccgggta gtagccagcc accttcgccg
    12361 tccatcgctt ctggcaggcg gctctggctt tcggcaaagg cgcggatttc gccatcctgc
    12421 acctccagcg aaccgtgcac cacctcgttt tccagcacca gcttaacgtt attgataatc
    12481 atgaagaggc tcccattggg tgcaggcggt cggcgacgtc attacgtaca gcttcgtcat
    12541 ggaagatgcc gacgatggct gcgccacggg ctttggcttc gtgaatcagt tccaccaccg
    12601 cggcgctgtt tttggcgtcc agcgaggcgg taggttcgtc gagcagcaga atggggtagt
    12661 cgacgataaa gccgcgggcg atgttgacgc gctgctgttc gccaccggaa aatgtcgatg
    12721 gtgccaggtg ccacaggcgt tccggcacgt tcaggcgggt gagaagacgc gcggctttag
    12781 cggcgcaggc ttcacgcgga acgcctgtat cgagcagcgg ctgcatcacc acttccagtg
    12841 ctgagatacg cgggatgacg cgcagaaact ggctcaccca gccgacggtg gttttgcgga
    12901 tttccaccac tttgcgcgct ggcgcggtga ccaggtctac ccactcgtca ccgtgtttga
    12961 tctggatttg accttcgtcg ggtagatagt tggcgtacag cgagcgcagc agggttgatt
    13021 tgccgctgcc ggagtgaccg tggagcacca cgcattcgcc tgcgttgacg gtgagcgagg
    13081 cgcgttgtaa gacgggcagg cgcacgccgt tttgctggtg caggatgaag gttttactga
    13141 cgttttgtac gttaatcatt tttcgcctcg tggttcgcat attggggagc ggtgcgcgca
    13201 ttgcccctca ccctaaccct ctccccgaag gggcgagggg accgattgtg cgcggtgttg
    13261 aatattgcgc cggttttctc cctctccctc cgggagaggg ccggggtgag gggatcagcg
    13321 cgcacggtgt tgcattcaat tctgcaaaac cgatgacacc agcagttgtg tatacggatg
    13381 gtgcgggtcg tcgagcacgc ggtcggttaa cccactctcc accacttgcc cctgcttcat
    13441 caccagcaaa cggtccgcca gcaggcgggc gacgcctaaa tcgtgggtga caatcactac
    13501 cgcgaggttc agctccacca ccaggccgcg cagcaggtcg agcaggcgtg cctgtaccga
    13561 cacatccagt ccgccggtcg gctcgtccat aaacaccagc ttcggatgcg tcaccaggtt
    13621 gcgggcgatc tgcaaacgtt gctgcatgcc gccggaaaag gtggtcggca ggtcgtcgat
    13681 ccggttggcg ggaatttcca cctcttccag ccacttctgc gcggtggcac gaatatcgcc
    13741 gtaatgacgt gccccggtcg ccatcagccg ctcgccgata ttgccgcctg ccgacacctg
    13801 gcggcgcagg ccgtcgagcg gatgctgatg cactacgccc cattcggtgc gcagcaggcg
    13861 acggcggtcg gccttgctca ttgcgtacag cgaacggctc tcatagtgaa tctccccctg
    13921 ctgcggcgtc aggcgcgcgg agattgattt cagcagcgtg gtcttcccgg agccggactc
    13981 cccgacaatg cccagcactt cccccggcca taaatcaaaa gagacatcgc taaagccttt
    14041 gcccggcgcg taaaggtggg tcaggttatt gaccgaaagt aacggttgat tcattggctt
    14101 ttcgcctcgc tctgttggcg gcaataatcg gtatcggagc agacaaacat gcggtttccg
    14161 gcgtcatcca gcaccacttc atcaagatag ctgtgggtcg atccgcagat ggcgcatggc
    14221 tcatcccact gctgaacggt gaacgggtga tcgtcgaaat cgagactttc cacgcgggta
    14281 aacggcggca ccgcatagat gcgcttctcg cgccctgcgc cgaacagctg cagggcgggc
    14341 atcatgtcca ttttcgggtt atcgaatttc gggatcggcg acgggtccat cacgtagcgc
    14401 ccgttcacct tcaccggata agcataggtg gtggcgatat gaccgaagcg ggcgatatct
    14461 tcatacagtt tcacctgcat cacgccgtac tcctccagcg cgtgcatagt gcgggtctcc
    14521 gtttcgcgcg gctcgataaa gcgcagcggt tccgggattg gcacctggaa gataatgatc
    14581 tgatcttcgg tcagcggcgt ttcggggatg cggtgacgcg tctggataag cgtcgcatcg
    14641 tccgtacgtt cagtggtgtt taccccggtc acgcgcttaa agaagttgcg aatcgacacg
    14701 gcgttggtgg tgtcatccgc accctggtca atcaccttca gcacgtcgct ttcgccaatc
    14761 acgctggcgg tgagctgtat gccgccggtt ccccagccgt atggcatcgg catctcgcgc
    14821 ccgccaaacg gcacctgata accggggatc gccaccgctt ttaagatggc gcggcggatc
    14881 atgcgtttgg tctgctcgtc gaggtaggca aagttgtagc cgctcagatt agccatggtt
    14941 cttctcctgt tgcagacgtt tgagtagctc cagttcggcc tggaaatcga cgtagtgggg
    15001 gagtttgagg tgcgagacaa agcctgcggc ttcgacgttg tcggcatgtg ccagcacaaa
    15061 ctcttcatcc tgcgccgggc ctgtcgcgtg ctcgccgtat tccggagcct gcaacgcacg
    15121 atcgaccagc gccattgcca tcgctttgcg ctcgctcatg ccgaatacca gcccgtagcc
    15181 gcgcgtgaag tgcggcggct catccggcgg gtcgataaaa ccgttgacca tttcacactc
    15241 ggtcatcagt agttcgccga cgtttaccgc aaatcccagc tcttccggca caatcgacac
    15301 atcgatgtaa ccactgcgga tctcgcccgc gaacgggtga ttgcgtccgt aaccacgctg
    15361 ggtggagtag gccagcgcca gcaaatagcc ttcgtccccg cgcattaatt gttgcaggcg
    15421 ggaagaacgt gaacaggggt aaaccggcgg cgtgcgggtg atgtcatccg gctgtgctcc
    15481 gctatcctct tcaaacttcg ccagcccctg acgcgccagc aggctgaaaa cgtgcggcga
    15541 cggctgttgt tcgctgtcgg cggtggtcag cgtcggcgct tcgccgtttg ccagcagagt
    15601 gaaatcgagc aggcgatggg tgtagtcgta ggttgggcca agcagctggc cgccgggaat
    15661 atctttataa accgctgaaa tacgccgttc gagacgcatc tccgtggtgt cgagcggctc
    15721 gcttaccgcc agtttcgtca gcgtggtgcg gtaggcgcgc agcaggaaaa ttgcttcaac
    15781 gttatcgccg ctggcctgtt tcagcgccag cgccgccagt tcgcggtcgg caatgccgcc
    15841 ttctgtcatc acgcgatcca ccgcgaggtt aagctgctgt tcaatctggg cgatgctcag
    15901 ttcgggcaaa tcggtatcgc cccggcgtcg gctctcttgc agggcgtggg cggcgtcgat
    15961 cgccttttcg ccccctttca cggcaacgta catcagcaca cctccacatg agtggttcgc
    16021 gggatagcca gcaggcgctc gccgcaggtc aggattaggt cgatgccgag cgggaacggg
    16081 tgcgggcgct cggtgagttc gtgcagaatg cactctggca gctgcggagc gatcattcgt
    16141 tcttcggcaa tgcccgcgcc ggtgagacgc aacatgcgtc cgccgctcag gctggcgacc
    16201 tgtaaaatca gcgtcgcgcc cgcttccggc gcaacggcgg tgccggtgga aagggcgttg
    16261 agctgttcgc tggaaatcgc ctcatccgtc accgcgaagg tcgcctgttc cggctggctg
    16321 accagcggcg cgttggtatg aaaacgcagg ctctgattga cgatatcgtt acttaatggc
    16381 gtggcaagcc acaccggcgt gtcgttatcg gccagcgtca gcagcacgct ggtggtggcg
    16441 atattcagcg gctgccagcc gcgtttgagc tgatgcaggg cgacaatcac gcccggctcg
    16501 ctcatcgcct ttaacaggcg acgaaaactg tgctgggcat cctgcacggg aagcataaaa
    16561 gcggtttcca gggtcatgcg ttgtctccgc gaaccatcgt aaagaagtcg acccggctgg
    16621 cgttcacttc ggcctggcgt gcggcaatgc gtgccatacg gtcagcgtcc agcggggcaa
    16681 taagggtttc tgataagttt tgaaaatagc gagattgctg catcagcgcg tcaatcagcg
    16741 cgcagcgttc ggcgtgctgt ttgtcgcgcc ccagcaccca gctgtagccg agcgtgccgt
    16801 cagtcaggcg tacggcggcg cgggtgagcg tggcgtcgcc ggcaaaaaag cgttcgccgg
    16861 tgccgcccat ccgcgcctga atctgcacca ggccggtttc ggcggcgcgg ataacctcat
    16921 agtcggcggt gatgttcagt gctttcaggc gggctgccag ttcagccggt tggctgtgcg
    16981 ccagtacgga catccagtgc tggcgggtcg cggtatctgc gtgcattcag tgctccatag
    17041 tgaattcaat catgtcggcg cgcgtcaggc tgacggagta ctccgccggg ctgctttcac
    17101 cgtcacggtg gttaagggtg cgcacgcaca gcagcggcga catattcgga atttcaagac
    17161 gctggcactc tttggcctgg gcgcgacggg cgctgatccg cgtctggctg cggcgcagcg
    17221 caattccggt ttgctcgcgc agaaaatcgt gcagcgaacc gctgtcgaag cgttgcagcg
    17281 tcggccaaag ggtgaggtcc gcaaagtagt ggtcgattaa acagagcgcg acgccgttga
    17341 cgcgacgcag ggtgcgcaaa tggataacgt tctcgccctc ggtaatcccc agcgcgtcag
    17401 cgacgtgacc ggacgcgggg cgcaataccg acagcagttt ttcgctggtg ggatggctac
    17461 cctggtccag caggttctgg ctaaaacgcg cctgggcgtt gagcgggtaa tcgaacgggc
    17521 gcatcagcac cagcacgccg acgccctgac ggcgctgtac ccagcctttt tccaccagtt
    17581 ggtcgatggc gcggcgcagg gtgtggcggt tcacctcaaa gcgcgccgcc agttgctgct
    17641 cggcgggaag atagtcgccg cagcggtagt gttgacgaag ctcctgctca agttttgcgg
    17701 ctatctcctg atagcgtgtt gggtagctgg tcggatgtgt agacaagtgc atagatatca
    17761 atgcctcgct tatcagataa agtgcttacg caaccgttga gagaggaaat ccagcaggct
    17821 gaccgtgacg atgataagca ccatcagggc gcaggtttgt tggaactgga aaccgcgaat
    17881 cgcttcccac agggtgacgc cgatcccgcc tgcaccgacc atgccgacga cggtcgccga
    17941 gcggacgttg gattcgaagc gatagaggga gtaggagatc agcagcggca tcacctgcgg
    18001 cagcacgccg tagaggatct cttcgagctt gttggcgccg gtggcgcgaa tgccttccac
    18061 cgggccgggt tcaatcgctt ccaccgcttc ggaaagcagt ttggagagca cgccggtggt
    18121 gtggataaac agcgccagca cgccagcgaa cgggccgagg ccgacggcga ccacgaacag
    18181 catggcgaag accatttcgt taatggcgcg gcaggcgtcc atcaggcggc gaacgggctg
    18241 gtaaacccac cacggcacca ggttttcggc gctcatcagg ccgaagggga tggagagaac
    18301 caccgccagc gccgtgcccc agacggcgat ttgcagcgtg acggccattt cggtgaggta
    18361 atcctgccac tggctgaaat cgggcgggaa gaagtcggcg gcgaacgtcg ccatgttgcc
    18421 gccgtcttta atcagcgtga gcggggccat ttccgcgccc tgccacgaga cgaccaacac
    18481 ggcgagaaca acggcccagc tcagaagcga gaaccagctg cgcttgggtg gggcgatggt
    18541 gatggtttgc atgtttggct ccggtctgta ggcctgataa ggtgcttgca ccgcatccgg
    18601 catcaacgcc tgcacattgt cggatgcgac gccggggcgt cttatccgac ctacgattta
    18661 ctgcaccgct ttactcaccg aactcatcgc gcttaacgcg ttgttcaggc ggtccaggtc
    18721 atccagctgc gcctgaatct cggtggtttt tgccagcttg tcctgctcat tcagtccttt
    18781 attgcttttc acgccctgca tctctttaaa cagcgcgagc tggcgaatcg gcaccagttg
    18841 caggtcgctg gaagcgcgga acggcgccca gcccaggcgt tccagcaccg ctttttcttc
    18901 cggcgttttg ccgtagttca taaagaagtc gtagatcttg tctttggtgg tttcggaaag
    18961 gtttttacgc cagacgatcg gatcgcctgg gatcagcggc gacttccaga tcaccttcag
    19021 ttctttcagc ttctctggcg cggaggtttt cagcttgtcg aggttttcgg tgttgttggt
    19081 ggcaacatcc acctgcttgt tggcgacggc cagcgcgttg gtttcatgcc cagcgttgac
    19141 ggtgcgctta aagtcgctgg cggagatatt gtttttggca aagacgtagt aaccggggac
    19201 gaggaagcca gaggtggagt taggatcgcc attgccgaag gtgagatctt tccgcttcgc
    19261 cagcagatcg ttcaggttgt tgatcggact atctttgttg acgatcaaca cgctccagta
    19321 acctggcgat ccatccgccg cgaccgtctg ggcgaagacc tggccgttgg cgcgatccac
    19381 cgcttccatt gccgacaggt tgccgtacca ggcgatatcc actttattga agcgcattcc
    19441 ctggataatg cctgcgtagt ctggggcaaa gaaggcgttc accttcacgc ccagcttctt
    19501 ctccatatcc tgtaagaatg gcgtccattg cggtttcagg ttttgctgtg attccgttga
    19561 aataatgccg aaattcagcg ccttttcctg ctcttcggcg tgtgccgggc ttaacagggt
    19621 gctgaggctg aacatgctgg tgaaggccag cgaggcaatt atcttagcgt tcattcgttt
    19681 tcctcaatga tggggatgtc aggcagcttt cgcgttctct tcgatgcgat taatgctgcg
    19741 gtagagatgg tcaaaacgtt cgttatcaaa ctgttggctg ctgccgtcgt agaaaacgtg
    19801 cccctggcgc agggcgacga tgcgttcgca gtagcgcagg gcgtaatcca cctgatgcag
    19861 cgtgacgacc acggtgatgc cgtcgttctg attgatgtcg cgcagggtgt ccatcacgat
    19921 gcgggcggat tccgggtcca gcgaggcgat gggttcatcg gccagaatca ccttcgcctg
    19981 ctgcatcagc gcgcgggcaa tcgccacacg ctgctgctgt ccgccggaga gggtggaaac
    20041 gcgctgatgg gcaaaatgca ccatgccaac gcgggtcagc gcctgtaacg cgcgctgttt
    20101 ctgctcgccg gtgaaccagc taaaacaggt gcgccagaac ggcgtgctgc cgagcgcgcc
    20161 aatcagcacg ttctccagta cgctcaggcg gttcaccagg ttgaattgtt ggaagatgta
    20221 gccggtgttg gcgcggcttt tgcggatatc gcgcgccaga cggccttcgc gctggactgt
    20281 gcggcccagc agctcgatat ggctgccggc ggatttatcg ccggtaatca aaccgcttaa
    20341 gtgacgtaaa agggtggatt tgccggaacc cgacggccca agcagagcca ccatttcacc
    20401 gtgatgaatg ttcagatcaa ccgcatgcag cgcctgatgc tgattgaagg ttttggcgag
    20461 cttctcgaca cggataatcg tttgcatgat gcagcctccc taaaaaagtg gctccatcgt
    20521 ggcggattat tgtgacgatt tggttaatta aaagtgacta acagatgaag agttaacggg
    20581 aattcgatga cagtgcggga gggcgggcct cccggcgggt tattgcgttg gttgttgttt
    20641 gacgacatta atcatccacg gtacgccaaa tttatcggtg actttgccaa agccatgcgc
    20701 ccagaaagtt tcctgccagg ccatttcgat ttttccgtta gcggcaagat tgtcaaacca
    20761 gcgttttcct tcttcgacct gttgcgaatc gagcaccagc gtaaagccgg agtagctggc
    20821 ttttcctgac ggtatagcat cgctcatcat gatgtcgctt ccggcaatgc gcacgttggc
    20881 gtgggcgatg gcggtatcgg gaaattgcat tccggaaggg cagttctcgg cgctgtcctg
    20941 cgctgatttt ggcatttcgc cgaagctgat tttatagagc agttcagcgc ccaacgtacg
    21001 ttgataataa gcaattgcgt cggcacagtt accggcaaaa gagaggtagg gacttaacgg
    21061 catgatggtg acctcagtta agagaagcca gttaagtgta gttcgaaatt tatacagatg
    21121 agagagaagc ctgataagac gcggtcagcg tcgcatcagg cagtcggcat cgttgccgga
    21181 tgcggcgcga gcgccttatc cggcttaccg atcacaatac aatcagttct ttttcacaaa
    21241 ctcagatttc agtttcatcg gaccaaaacc gtcgattttg caatcgatgt tatggtcgcc
    21301 ttcaaccagg cggatgtttt tcactttggt gccaattttc agcatcgaag agctaccttt
    21361 caccttcaga tctttaatga tggtaacgct gtcgccgtca gccagcagat tgccgttagc
    21421 atctttaacg atcagctcgt cgctttcctg tgcaggttct gcgtcgttcc attcgtaggc
    21481 acattccggg cagatgtaca tgccgttatc ttcgtaagtg tattcggagt tgcattttgg
    21541 gcagtgtggt aatgacatgt ggatttcctc aaaagtcagc agggcaaaag cgccaaaaaa
    21601 cggcagattg ccgaaaaagg ccgcaattat acacaaaatc cctgagtttg tcgggggtac
    21661 tgctgatttt agacgacgat cgttttcatt tgaaaggaaa cgcaaacaga aaataatctt
    21721 tttgcctcca tttattattt
//
LOCUS       DAJOWB010000078        21464 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJOWB010000078 DAJOWB010000000
VERSION     DAJOWB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.078"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            235..735
                     /gene="tssB"
                     /locus_tag="OHO85_003134"
     CDS             235..735
                     /gene="tssB"
                     /locus_tag="OHO85_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HCQ2672477.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            770..994
                     /locus_tag="OHO85_003135"
     CDS             770..994
                     /locus_tag="OHO85_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672478.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1045..2520
                     /gene="tssC"
                     /locus_tag="OHO85_003136"
     CDS             1045..2520
                     /gene="tssC"
                     /locus_tag="OHO85_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HCQ2672479.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2527..2940
                     /gene="tssE"
                     /locus_tag="OHO85_003137"
     CDS             2527..2940
                     /gene="tssE"
                     /locus_tag="OHO85_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCQ2672480.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGNQHYRVD"
     gene            2944..4794
                     /gene="tssF"
                     /locus_tag="OHO85_003138"
     CDS             2944..4794
                     /gene="tssF"
                     /locus_tag="OHO85_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCQ2672481.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYSSVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4758..5840
                     /gene="tssG"
                     /locus_tag="OHO85_003139"
     CDS             4758..5840
                     /gene="tssG"
                     /locus_tag="OHO85_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HCQ2672482.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5865..7145
                     /gene="tagH"
                     /locus_tag="OHO85_003140"
     CDS             5865..7145
                     /gene="tagH"
                     /locus_tag="OHO85_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HCQ2672483.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7142..7666
                     /gene="tssJ"
                     /locus_tag="OHO85_003141"
     CDS             7142..7666
                     /gene="tssJ"
                     /locus_tag="OHO85_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HCQ2672484.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7669..9000
                     /gene="tssK"
                     /locus_tag="OHO85_003142"
     CDS             7669..9000
                     /gene="tssK"
                     /locus_tag="OHO85_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HCQ2672485.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRTGRYRLNYADVRDLHSEEGDTRTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANVPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9005..9766
                     /gene="icmH"
                     /locus_tag="OHO85_003143"
     CDS             9005..9766
                     /gene="icmH"
                     /locus_tag="OHO85_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HCQ2672486.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9775..12540
                     /gene="tssH"
                     /locus_tag="OHO85_003144"
     CDS             9775..12540
                     /gene="tssH"
                     /locus_tag="OHO85_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HCQ2672487.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESEQDNTGAVPADEADREQPEETAETVSPVQRLAQLTAELD
                     ALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWLGDTIKGQDLA
                     IASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINM
                     SEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQ
                     AFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPA
                     LLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVVIEPEVTDEIMSRVTRA
                     ENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDAQNDESV
                     TKDETVL"
     gene            12537..13280
                     /gene="tagO"
                     /locus_tag="OHO85_003145"
     CDS             12537..13280
                     /gene="tagO"
                     /locus_tag="OHO85_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HCQ2672488.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKHRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLPVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13285..14697
                     /gene="tssA"
                     /locus_tag="OHO85_003146"
     CDS             13285..14697
                     /gene="tssA"
                     /locus_tag="OHO85_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HCQ2672489.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEGFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14806..18240
                     /gene="tssM"
                     /locus_tag="OHO85_003147"
     CDS             14806..18240
                     /gene="tssM"
                     /locus_tag="OHO85_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HCQ2672490.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALMWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNQ
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLTDQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18251..19603
                     /locus_tag="OHO85_003148"
     CDS             18251..19603
                     /locus_tag="OHO85_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HCQ2672491.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLEPQLKSNTENAAQTTVRTLIYAPETQASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPVESLLTQYHDARAQKQNTDTLEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            19627..20109
                     /locus_tag="OHO85_003149"
     CDS             19627..20109
                     /locus_tag="OHO85_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCQ2672492.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            20153..21067
                     /locus_tag="OHO85_003150"
     CDS             20153..21067
                     /locus_tag="OHO85_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672493.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
     gene            21077..>21464
                     /locus_tag="OHO85_003151"
     CDS             21077..>21464
                     /locus_tag="OHO85_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672494.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFTSFSFLNIHVILLTIIKSFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVNANYHPCGNN"
BASE COUNT         5161 a         5758 c         5811 g         4734 t
ORIGIN      
        1 atacacctga cgcgaattgc acaggttcat acaatctatt taacgcttgc agaatacccc
       61 tctttacgat cgataaaaag tccttttctt atttattttg aagcgtaaaa tgcggggtga
      121 tgaaggagtg gtgattttat tttcaccgcc atcaaggcgg catttccgat attttttgcg
      181 gtacgaatat gccgtgttca tggcctgtac gagataacat gacggatcac ttttatgagc
      241 aaaaaatttg aaggcagcgt cgcgcctcgc gagcgtatta atatatcgta tgtcccaaaa
      301 acagatggcc agacggctga agtggaactt ccgcttaaca tgctcgttgt cggcgatacc
      361 ggaaatacgc aggaaacatc ctcactggat gaacgtcagg cagtttccgt taacaagcac
      421 aatttcggtg cagtgatggc tgaagcagcc attggcctca attttactgt ccccgccacc
      481 ctgaagggaa gcacaacaga cgatgaactg aatgtggcgc tgaatatcaa atcactggat
      541 gatttttcac cagatagcgt ggcccggcag gttccggaag tgaataaatt gctggaactg
      601 cgtgaagcac tgactgcact gaaggggccg atgggtaacc ttccggcatt ccgtacccag
      661 ctccaggcat tgctggaaaa tgaagagtcc cgcgagcagt tgctcaaaga aatcggtctg
      721 gtgagcaata aataaccacc actatttcat taaacagggt tatccaggca tgacggcaca
      781 caaaaatatc tccgggacat accacctctc tgttgcagac attctgcagg tggtttacca
      841 ggtatgtttc agcccgtccg ttgaaatcaa ccaggatggt gtggctgcgc tgattacaac
      901 actggatcgc cgtatcagtg atctattgga tgagattatc catttttgtg agtttcagca
      961 gagcgcgtcg cactggcagc gcgtacttca ctgacggcga aaagccggtt tcagaaacac
     1021 agctaaaaca gaaggatttt cagtatgtct ttacaggaag aggaactcgt atccagtcat
     1081 gccgggcagc ctgagcaggc atcgtcgctg cttgatcaaa tcatggcaca gacccgtatt
     1141 cagcccggtt cggaaggtta tgacgttgcc cgccagggtg tgacagcctt tattgccagc
     1201 atcctgcaat ccactgcatc tgccgaaccg gtcaataaac tggccgtgga cagcatgatt
     1261 gcagatattg atgaacgcat cagccgccag atggatgtca tcatccatgc gcctgccttt
     1321 cagcaggtcg agtcgttctg gcgctcactg aaaaccatgg tggatcgcgt tgatttccgt
     1381 gaaaacatca aggtcaacgt gcttcatgtc accaagcagg aacttctgga agactttgag
     1441 tttgcgccag aaattattca gtccggattt tataagcacg tttattcgtc cggtttcgga
     1501 cagtttggcg gtgaacccat cgctgccgtg ctgggcgctt atgagttcaa aaataccgcg
     1561 cctgacatga aactgctgca atacgtcagt gccgtgggcg caatggcgca cgcaccgttc
     1621 ctgtcctccg tttccccgga gtttatgggg ctgaactcgt ggaccgaact gcctaatatc
     1681 aaagatcttt atgccatctt tgaaggtccg gcctacacca aatggcgcgc cctgcgtgac
     1741 tcggaagatt cgcgctattt agggcttacg gcaccccgct tcctgcttcg ccagccctat
     1801 tcaccaacag ataaccctgt taagaacttt aactattacg aagatgtcag ccagaaccac
     1861 gaagattatc tgtggggtaa tacggcctgg atgctggcat gcaatatcgc agacagtttt
     1921 gccaaatacc gctggtgtcc aaacattatc ggtccgcaaa gcggcggcgc agtgaaagat
     1981 ctgccggtgc atctgttcga aacgatgggg cagattcagg ccaaaatccc aaccgaggtg
     2041 ctggtcaccg atcgccgcga atttgaactg gctgaagagg gttttatcac ccttaccatg
     2101 cgtaaagact ctgataatgc agcctttttc tctgcaaact cggtacaaaa accgaagcac
     2161 tttccgggaa aagatgcaga aaccaactat aagctgggta cgcagcttcc ttatctcttc
     2221 atcatcaatc ggttagcaca ctacatcaag gtgttgcagc gtgaacaact ggggtcatgg
     2281 aaagaaagga gcgacttaga aagagagctt aatacctgga tccgtcagta tgttgccgac
     2341 caggaaaacc cgcctgcaga cgtgcgcagc cgcaaacctc tgcgcgctgc caaagttgag
     2401 gttatggatg tagaaggcga acccggctgg taccaggttg cgctaagcgt gagacctcat
     2461 tttaaattta tgggggcaaa ttttgagctt tccctggttg gccggttaga cagggagtaa
     2521 cacaccatga taccggatcg ggaaagaacc acaggttctc tttttgagcg tatggaagcc
     2581 tcttcggcca gaaaccggca gggaggcagt attcattcgc tcaggcaatc cattcgccag
     2641 aacctacgaa acattcttaa tacccggtcc ggtagttgcc ggggtgcgcc tgagctgggc
     2701 attgatgaac cggaaggggc tgaaaacttc cgcgaatcca tgagtcgggc catcgagcaa
     2761 tgtatagaac gctatgaacc ccggatctca cacgctgaag tgcaggcagt ggtttcatcc
     2821 gcctccagcc cgctggatat gacgtttcat attacagcct gggtgacatt taacgaaacg
     2881 catgaggtgc ttgaatttga tatggcaccg aatggcaacc agcattaccg ggtggactga
     2941 caaatggaat ttgaagaacg ctatttccgg gaagaactcg attacctgcg ccagcttagc
     3001 aagctgctgg caacggaaaa accccatctg gcccgcttcc tggccgaaaa agatgcggat
     3061 ccggatattg aacgcctgct ggaaggggtg gcttttctta ccggcaatct ccgccagaaa
     3121 attgaggatg aattcccaga actgacgcac gggcttatta agatgctatg gcctaattac
     3181 ctgcgtccgg ttccggcaat gacccttatt gaatatacgc cggatatgga taagtcttct
     3241 gtaccggtgt taatcccccg taatgagcag tttacaacca acgccgggga aatcagagtt
     3301 gatgaagtgc tgccctctga tgctaaaaag gaggagccgc ctccctgtac cttcaccctc
     3361 tgccgggata tctggctgct gcccgttcgc ctggagcaga ttgaaaaccg cagtacgacc
     3421 cgtaatggtg ttatcaacat caccttttcg gtcgcaccgg gaacggactt ccgcacgctg
     3481 gatctgaaca aacttcgctt ctggctcggc aatgacgaca actatacccg tgaccagctt
     3541 tatttatggt tctgcgaata cttgcagggt gccgacctga ctgtgggtga acagcatatt
     3601 cgcctgcctg agtttatgct aaaagctgtc ggttttgagc cgcaggatgc catgctgccc
     3661 tggccgaaaa acgtccacag cggctaccgg atccttcagg agtatttctg ttaccccgat
     3721 gcgtttctct tttttgatct ttgtggttgt ccggctttgc ctgacggatt gcaggcggaa
     3781 ttctttaccc tgcaactgcg tttttcgcgc cctttgcccg tggacatccg gctgcgccgc
     3841 gattccctgc gcctgtattg cgcacctgcc attaatttat ttatccacca tgcagaagcc
     3901 atcacgctgg acaaccggcg ggcagactat ccgctggttc ccagccgcca ttacccacaa
     3961 cattacgatg tattttccgt taacagtgtg gtgagccagg tccaggatat gttcaggaaa
     4021 aaagatctgg ggcgtcctgt ttcgacgcag gccgcgcgcc agtggccagc ctttgaaagt
     4081 ttcagccatc agatggaata cagccggaag cgggaagtgg tgtactggca tcaccggacc
     4141 aaaacatccc tgttccatcg cggctttgat catacccttg cctttataca tgctgatggc
     4201 agttatccgt cagacgaatc tctgctcagt aatgaagtgg tttcggtatc gctgacctgt
     4261 accaaccgtg agcttccgtc acaaattcgt tccggcgata tcaccggcac aaccggtaaa
     4321 aatgcggctg ttgcttcatt tcgcaacatt acccgcccga cgcaaccact ctggccggtc
     4381 attgatggca gcctgcactg gtccctactc tccgccatga acctgaatta tctgtcatta
     4441 ctggatacgg acgcgctgaa gcaggtcatc gccaactttg atcgccacgc aatccatcat
     4501 ccgcagacgg cacggctgtc acaacaaaag ctggatgcca ttgagcgtct ggagacccgc
     4561 cccgttgatc gcctgtttac gggtattccc gtccggggac tggcctccac gctgtatctg
     4621 cacccggagc cgtttgtctg tgaaggggaa atgtatctgc tcggtacggt gctttcgcat
     4681 tttctgtcgc tgtactccag cgttaactca ttccacatgc tgaccgttgt gaacacagaa
     4741 agccaggaga catggaaatg gacggaaaga atcgggcagc atcctcttat ctgagtccgg
     4801 gaaatccccc ggcagataaa gagcagaatg atccgctggc acaggttttc cataatgcct
     4861 gctcttacaa tttttttgcg atggcggagc tgttgcaccg cctggcaaag ggtgaaaagg
     4921 ggacgccaga attatccctg cgtgacgatc cggcacagga aaccctgcgt ttcagcgccg
     4981 atgccagcct tgccttcccg tgcagtgata tcagtgcgct gaaacgggat acgtcagggg
     5041 cattcaggat gaccaccacc tttatggggt tacaggggag ccagtcgcct ctccccggtt
     5101 attacctcga tcacctggcc tggaaagcgg tacatgaaca aagtccggta ggtgattttc
     5161 tggatatgtt cagccaccgc ctgacccagt ttgtctggca tatctggcgt aagtaccgct
     5221 atcacatcag ttttcgtaac ggcggtgtgg atgcgttctc gcagcgcatg tactcccttg
     5281 tcgggctggg ccaccgccag cttcgcgata agctggcgat taatcacagc aaaatgctgg
     5341 cgtattccgg gatcctggca aatccgggac gatccccgga aataatctgt ggcctggtgt
     5401 cccactgctt tgatttgtca gaagtgacgc tgcaaaactg gcaacgtcgc aaggtggata
     5461 ttgaaccgga tcagcaaaat agtctgggga gctattctct taaaaatggc gaaaaactgg
     5521 cgggaagatc ggtactgggc aactttgttc tgggtacacg agtgcccgat ctcagcggaa
     5581 aattccagct cagtattacc agcctgaccc gaaaacagtt cctctctttt ctgccgtccg
     5641 gcgaaaactt cctgccactg acgatgttcg tgtccttcat cctgcgcgat cagcttgcct
     5701 gggacttaca tctggggctg gcgccggagc aggtgggcgc aatgcgcctg ggcgataaca
     5761 aaagtgcgct gctgggctgg accagcttcc tcggcacacc ggaagaacga ccctcagtca
     5821 ccatcagggt acggtcgtaa attatgataa tcacggagca gaatatgccg gaagagaaac
     5881 tacagacgct ttcattgcag gtcattaacg gcagtgagct ggaaagcgga cgggcggcgc
     5941 gctgtctgtt cacacagcag ggaaatgtcg gccatggccc cgaatgccac tggtcggtac
     6001 aggatcgtca gcagagcatt ccggcccagg cttttaccgt tatcctgcac gatggcacat
     6061 tttgtctgcg cccgcagacg gcacaactgt ggctgaatca ggcaaaagtc acagcaacat
     6121 cagacctaat acagttgcgc cagggcgatg agatccagat cggacggctg atggtgaggg
     6181 ttcatctgaa ccggggagat attccccatt acgatgagga aatggccact cccgaaacca
     6241 tcgttaccaa tcgcgatatg ctcacggata ccctgctatc aacggagggt gcgccacact
     6301 atccgggaat gactcaccgg caccagcttg cagacaccgt ggtaaacggt ttttctgccg
     6361 atccactcca ggcacttcag tccgaaagcc tgattaccac gggcgatccg ctttcaggca
     6421 ttgcggctgt ccggccatcg gcaccgctgt ccgatccggc aagtaatggg gggatcaata
     6481 ctccgtttat ggatctgccg cccatttatg ccagccccgg cgatcgtaat gacgacgtct
     6541 ctgcggcaga aatggcgcaa cgccaccttg cggtcacccc cttacttcgc ggtcttggcg
     6601 gctcgcttac catgagcaat tccgacgatg cggatgattt tctggaggag gccggacgaa
     6661 cgttacaggc cgcaataaaa ggtctgctcg atttgcagca gcagcgtaac agcctctcag
     6721 acaaacattt gcgcccgctg gaagataacc cgctgcgcct gaacatggat tacgccaccg
     6781 cgctcgacgt aatgtttgcc gaaggtaaaa gcccggtaca tctggcggct cccgccgccg
     6841 tcagtgaaag cctgcgcaat gtccgccacc acgaagaagc taaccgggca gcaattgtgg
     6901 agtcgcttcg tgtcctgctg gatgctttct caccacaaaa tctgctgcgc cgctttgtgc
     6961 agtaccgccg cagccatgaa ctgcgccagc cgctggatga tgccggagca tggcaaatgt
     7021 acagccatta ttacgaagaa ctggcctccg atcgccagca ggggtttgag atgctgttta
     7081 acgaggtcta cgcccaggtc tatgaccggg tgcttcgtga aaaacagcgg gagccggaag
     7141 catgaacagg aaggcgtttc tggcctgcgt gctgatgtgc gtcatattaa ccggctgcga
     7201 aacggcgaaa aaaatcagcg aggtgatcaa aaacccggat atccaggtcg ggagcctgaa
     7261 ggagcaacct tcggaaataa ccgtcaccct gctgaccgaa ccggacacca ataccaatgc
     7321 ggagggtgaa tcagccgccg tggatgtgca actggtgtac ctgactgacg actcaaaatt
     7381 gctggcagcc gactatgacc agatagccag tacgccgcta ccggatgtgt tgggtaaaaa
     7441 ctatatcgac caccaggatt tcaacctgtt gccggacacc atcaaaaccc tgccgccggt
     7501 aaagctggat gagaaaacgc agtttattgg tgtcgtggcc tatttctccg atgaccaggc
     7561 cactgaatgg aaacaaatcg agacggtgga aggaaccggc caccattacc gcctgctggt
     7621 gcatgtacgt caaagcagca tcgaaatgaa aaaagaggat gaataattat ggcaaccacc
     7681 cgcaacaagg tgatgtggca ggaagggatg ctgatgcgcc cacaccattt ccagcaacag
     7741 cagcgttaca acgactacct ggataaccag cgtttccggg ccatgaatga tttatcctgg
     7801 ggatttaccg aactcaccct caacaatgaa ctgctggcgc agggtaagat catgattgac
     7861 agcgcgtcag gcacactgcc cgacggcacc gtcttttcta tccccgacca ggacgcactg
     7921 cccgatccgc tgcacccgca aaattttccg gacgaaagaa gccgcaatat ctacctcgct
     7981 ctgccggtcg ccagtgatgt gagaaatgaa atcagcgacg gacggcgaac cgggcgttac
     8041 cggctgaatt atgccgatgt ccgggatttg cattcagaag aaggtgacac gcgaacattg
     8101 acgctgggtc aactgacgcc gcgcattatg agcggtgcag aagatatgag cgcctatatt
     8161 acgctcccgc tttgccgtat cagcgatcgc catgccgatg gctccctgac gttggatgac
     8221 gattttatcc cctcctgcca gaatattcag gtcagtaaga aactgcgtgt ttatctcaaa
     8281 gaggtacagg gggccattgg cggacgggca agcgatctgg caaaccgtat tggctctccg
     8341 gcgcagagcg gcatcgcgga tgtggcggaa tttatgatgt tgcagttact taaccgtaac
     8401 cagacccggt ttacccatcg cgcccgccga tcccagctcc acccggaaga tttctacctt
     8461 gatcttgctg ggttgctggg tgaactgatg acctttaccg agccgtcgcg cctgccctgc
     8521 ccgcttgatg tgtatgatca tcatgatctg accaaaacat ttaaaacact gttaccggaa
     8581 gtcaaacggg cgctgcatac cgtactgtcg ccaagagcgg tcaatctgcc gctgcatctg
     8641 cgtgacggta tctggcaggc cgatatccat gacacagaac tgctgcaatc tgccaccttt
     8701 gtgctggccg tggcggcaaa cgtgccggtc gatcagatcc agcgtcagtt tatccagcag
     8761 tcgaaaattt cctcgccgga aaaaatccgc aatatggtca gtgtgcagat ccccggtatt
     8821 ccattgcgtg cccttatggt ggctccccgc cagcttcctt accattccgg gttcagctat
     8881 ttcgaactcg acaagagtgg ccaggcctgg acagaaatgg ctgccgccgg ggccgttgca
     8941 ctgcatgttt ccggcagttt cccggatctg aacatgcaac tgtgggcgat aagagggtaa
     9001 gacgatggat gaggggtcac tgtctctgcc accatttacc ggacacgacg aaaaaagcca
     9061 gcgcaactac catctcgcgc ttcgtggcaa cagcctgaat ccgatgatcg acgcggccac
     9121 gccgctactc ggtatggtaa tgcgcctttc aaccatgaac agccagacga tgccggagca
     9181 tctttttgct caggtggtga cagatgttca ggccgtggag caactgcttc aggagcaggg
     9241 ctacgaaccg ggcgtgatta tctcgtttcg ctacatcctc tgcacgttta ttgatgaagc
     9301 ggcgctcggt aacggctggt caaacaagaa cgagtggatc aagcagtcat tgctggtcca
     9361 tttccacaac gaagcctggg gtggcgagaa agttttcatc ctgctggagc gcctgatccg
     9421 tgaacccaaa cgttaccagg atctgctgga atttctctgg atctgttttt ctctgggatt
     9481 tcgcgggcgc tacaaagtgg cagcacagga tcagggggaa tttgagcaga tctaccgccg
     9541 tctgtaccac gttctgcata aactgcgcgg cgatgcccct ttcccgctgt tacaccagga
     9601 caaaaaaacc cagggcgggc gttatcagct catcagtcgc ctgactgtta aacatatttt
     9661 ttgtggtggc gttgtcgtgc tggcgttgtt ttacctgttc tatctgttgc ggctggacag
     9721 ccagacgcag gacatcctgc accagctaaa caaattactt cgataaggac gtttatgatc
     9781 cagattgatc ttcccacgct ggtaaaacgg ctgaacctgt tctcccgcca ggcgctggag
     9841 atggccgcct ctgaatgtat gagtcagcag gcagcggaaa ttaccgtcag ccatgtgctc
     9901 attcagatgc tcgccatgcc acgcagtgac ctgcgggtta ttacccgcca gggcgatatt
     9961 ggcatggaag agttgcgcca ggcgctgacg gtagagaact acacaaccgc ccgttctgcg
    10021 gacagctacc cggcgttttc cccgatgctg gttgagtggc ttaaagaggg ctggctgctg
    10081 gcgtcggctg agatgcagca cagcgaactg cgcggcggcg tgttgctgct ggccctgctg
    10141 cattcgccgc tgcgttatat accgcctgct gccgcccggc tgttgaccgg cattaaccgt
    10201 gaccgtctgc aacaggactt tgtgcagtgg acacaggagt cggcggaatc agtcgtgccg
    10261 gatgcagacg gtaaaggcgc aggcacaatg acggacgcct ctgacaccct gcttgcccgc
    10321 tatgccaaaa acatgaccgc agacgcccgt aacggcaggc ttgacccggt actgtgccgc
    10381 gaccacgaaa tcgacctgat gatcgacatt ctctgccgcc gccgtaaaaa caacccggtg
    10441 gtggtgggcg aagcgggcgt gggcaaaagc gcactgattg aagggctggc gctgcgcatc
    10501 gtggcaggcc aggtgccgga caagctgaaa aacaccgata tcatgaccct tgacttgggc
    10561 gcattgcagg ccggggcgtc ggtgaagggt gaattcgaaa aacgtttcaa agggctgatg
    10621 gcggaggtca tttcctcccc ggtgccggtc attctgttta tcgacgaagc acataccctg
    10681 attggcgcgg gcaaccagca gggcgggctg gatatctcca acctgctcaa accggcgctg
    10741 gcgcgcggcg agctgaaaac catcgccgcc accacctgga gcgagtacaa aaaatacttc
    10801 gaaaaagatg ccgccctgtc gcgccgcttc cagttggtga aggtcagcga acccaacgct
    10861 gccgaagcca ccattattct gcgcggtctg tcggcggtct atgaacagtc tcacggcgtg
    10921 ctgattgatg atgacgcctt gcaggccgct gcgacattaa gcgagcgtta tctctccggg
    10981 cgtcagttac cggacaaagc gattgatgtg ctggataccg cctgcgcccg tgtggccatc
    11041 aacctgtcgt cgccgccgaa gcaaatctcg gcgctgacca ctctgagcca ccagcaggag
    11101 gcggaaattc gccagcttga gcgcgagctt cgcatcggac tgcgtaccga cacatcacgg
    11161 atgaccgagg tgctggtgca gtatgatgaa acgctgacgg cgctggatga actggaagcg
    11221 gcctggcacc agcagcagac gctggtccgg gagattattg cgctgcgcca gcagttactg
    11281 ggcgtggcag aggacgatgc ggcgccgttg ccggacgcag ataccgtgga ggatacgcag
    11341 ccagagtcag agtcagaaca ggataatacc ggtgccgtac cggctgatga ggccgacaga
    11401 gaacaaccgg aagagaccgc tgaaacagtt tccccggtac agcggctggc ccagctcact
    11461 gccgaactgg acgccctgca taacgaccgg ttgctggtct ccccgcacgt cgataaaaaa
    11521 cagattgcgg cggtgattgc cgaatggacc ggcgtgccgc tcaaccgcct gtcgcagaat
    11581 gaaatgtcgg tcatcaccga cctgacgaaa tggctgggtg acaccatcaa aggccaggac
    11641 ctggcgattg ccagcctgca taagcatcta ctgaccgcac gcgccgacct gcgtcgtccg
    11701 ggacgcccgc tcggtgcgtt cctgctggct ggccccagcg gtgtgggtaa aaccgaaacc
    11761 gtcctgcaac tggcagagct gctctacggc ggtcgccagt acctgaccac catcaatatg
    11821 tccgaattcc aggagaaaca caccgtctcg cggctgattg gttcgcctcc gggctacgtt
    11881 ggctacggtg aaggcggcgt actgaccgaa gcgattcgcc agaaacccta ctcggtagta
    11941 ctgctcgatg aagtggaaaa agcgcacccg gatgtgctca acctgttcta ccaggcgttc
    12001 gataagggcg aaatggcaga cggtgaaggc cgcctgattg actgcaaaaa tatcgtcttc
    12061 ttcctgacgt ccaacctcgg ctaccaggta atagtcgagc atgccgatga cccggaaacc
    12121 atgcaggaag tactgtatcc ggtgctggcc gacttcttca aacctgccct gctggcgcgt
    12181 atggaagtgg tgccgtatct gccgctgtcg aaagagacgc tcgccaccat tatcgctgga
    12241 aaactggccc gtctggataa cgtgctgcgc agtcgctttg gtgcagaagt ggtcattgaa
    12301 ccggaagtga cggacgaaat catgagccgc gtcacccgcg cggaaaacgg cgcgaggatg
    12361 ctggaatcgg tcatcgatgg cgacatgcta ccgccgctct ccctgctgct gttgcagaaa
    12421 atggcggcta acacggcgat tgcccggatt cggttgtcgg cagtggacgg cgcatttacg
    12481 gcagacgtgg aagatgctca gaacgacgag tccgtcacaa aggatgaaac ggttttatga
    12541 gcaggttgcg cgttttccgg cagacaggat ggtttatcgc gggcctgatg acgggcttac
    12601 ccgccactgc cgcgcccgca gaagcatcgt caatggccgg tgtggcggtc gccgtcgcca
    12661 ccacgacccc gccggatgcg acagccacgt tacaggccat gcagtcctgt cggcgggagt
    12721 ccgcggcgct ggagcggctt gactgctatg accgcctgct ggcaccgctg tccccgtcag
    12781 ggtttgacgg ggcgctggtc aaagccggtt ttgtgggcga agcctggacc cgcgccacgg
    12841 aacaggaaaa acaccgccag ggcaacacca cagagctgct ggtgacgcag gtgccggggg
    12901 aacgcccgac ggtggtgata accaccccgg ctatcggtca tgttccgcca cgcccggtgc
    12961 tgatgttcag ttgcgtggac aatatcaccc ggatgcaggt ggcgctgatg caccctctgg
    13021 atgtccatga tatcgccgtc actctgaatg ccgacaaccg ggcattgcgc agccactggt
    13081 ttgtgcgcga gaacggcacc ctgctggagt ccagccgggg gttatccggg attgatgaaa
    13141 ttaagcaact gttcggcgca aaaacgctgc cggtggatac cggcgcagac aatgccgccg
    13201 gaaagctgac ctttaacatt gacgggctgg caagggcgat tgctcccctg cgcgatgcct
    13261 gccactgggc gggagaataa aaatatggca atggacttac gcgatccgaa tgtctggata
    13321 tcgcacctgc tggaaaacct gccggaagag aaactggcat cggcgctgaa agatgacaac
    13381 ccgaactggg agtatatcga cggcgaaatc gtcaagctgg gatcgctggc ccacagtcaa
    13441 ctggatatcc cggaactcca gcgccggggg ctggtcattc tggcttcgga gagtaaggat
    13501 tttcgtctgc tggcgcatct gctgagaacc ctgcaacacg ccggtgatcc tcatctcgca
    13561 ttgcgcctgc tggcgctgta tgtggagcat tactgggcgg tggctgcgcc gcagaatatg
    13621 gcgcataaaa aacgctttgc cagccaggtc attaaacgct ttgaaacagg tatcgaaggt
    13681 ttttcacaga acgccgccac agcgcagcgc gatgcgctgt caggggaact ggcaaaactg
    13741 gcgcaatgct ggcagtcaca taacgccccg gaactggcac aggctactga tgatcttttt
    13801 gccctgtacc agcgtgcgtt taatcgtgcg gcaccggctc ctgtccccac tccggcggcc
    13861 tccggtagtt caccacagac caccgccacg tctgaaagcg gcgtgacgca acccagtgct
    13921 ccggctcccc aaatcgtcat cgacagtcac gacgacaaag cctggcgcga cacgctgtta
    13981 aaagtggcgg ccattttatg tgaacgccag ccggattcac cgcagggcta ccgtctgcgc
    14041 cgccatgcgc tttggcagaa catcaccagt acgccccagg cggaaagcga tggccgtact
    14101 ccactggctg cggtctccgc cgatatggtg gcagattacc atgctcagct cggtagtgcc
    14161 gatatggcgc tgtggcagca ggtggagaaa agcgtactgc tggctcctta ctggctggat
    14221 ggtcactgcc tttctgcaca gacggcgctg cgtctgggtt acaaacaggt ggcagacgcc
    14281 atccgcgatg aggtcatccg cttccttgag cgtctgcccc agcttaccgg gctgctgttt
    14341 aatgaccaca caccgtttat cagtgagcag acgaaacaat ggctggcagc ttcgcccgac
    14401 gccaaagttg caccggttgc gcaaatcggt gaggaatcga aggcagccag agcctgtttt
    14461 gctgagcagg gtctggaggc ggcgctgcga tatctggaca tgctacccga aggcgatccc
    14521 cgcgatcagt ttcaccgcca gtaccttgcc gcacagttga cggaagaggc ggggctggta
    14581 cagcttgcgc agcagcagta ccggatgttg ttcaggatgg gattgcagat gatggtggca
    14641 gactgggagc cgtccctgct tgagcagcta gaacaaaaat tcacggcaga acaataaaag
    14701 agaacaggag tttttgtgtt cagattacca acaccccgac tactcagcgg actcaaatca
    14761 gccctgcgac cggcgatgcc ccggtttaaa gtctctgctt tctggctgct gatactggcg
    14821 tggatttttc tgctggtgtg gatctggtgg aaaggcccga cgtggacgct gtatgaagaa
    14881 cagtggctca aaccactggc gaaccgctgg ctggcaacgg cggcgtgggg gattattgcc
    14941 ctgatgtggc ttaccgtccg ggtgatgaag cgcctgcaac agcttgaaaa aatgcaaaag
    15001 caacagcgcg aggaagccgt tgatccgctc agcgtggaac tgaacgccca gcagcgttat
    15061 cttgaccgct ggctgctgcg cctgcaacgc catctcgaca accgccgttt cctgtggcag
    15121 ttgccgtggt atatggtcat cggcccggcg ggcagtggca aaacgacact gctgcgcgag
    15181 gggtttccgt ccgacattat ttatgccccg gagggcgcac gcggcgcaga acaacgcctg
    15241 tacctcacgc cccatgtcgg taaacaggcg gtgatctttg atatcgacgg cacactctgc
    15301 gctcccgctg atgcggatat cctgcatcgc cgtctgtggg aacatgccct cggctggctg
    15361 aaagaaaagc gcgcgcgcca gccgctgaat gggattattc tgacactcga tttacccgat
    15421 ctgcttaccg cagacaaacg ccgccgcgag catctgttac agacgctgcg cagccgcttg
    15481 caggatatac gccagcatct tcactgccag ttaccggttt acgtggtact tacccggctt
    15541 gatttattgc agggttttgc cgccctgttt cagtccctga acagacagga tcgcgatgcg
    15601 attctgggcg tcacgttcac ccgccgtgcc catgaaaatg atgactggcg aacagagttg
    15661 aatgctttct ggcagacatg ggtggatcga atgaatctgg cgttgccgga tctgatggtc
    15721 gctcagactc acacccgcgc gtctttattc agtttttccc gccagatgca gggaagccgt
    15781 gaaccgctgg tgtcactgct tgagggtctg cttgatggcg aaaatatgaa cgtgatgctg
    15841 cgtggtgtct atctcacctc ttcgcttcag cgtggacaga tggatgatat attcacccag
    15901 tctgccgccc gccagtaccg gctgggcaat aacccactgg cgtcctggcc cctggtggac
    15961 accgcgcctt atttcacccg cagcctgttc ccgcaggcat tactcgcaga gcctaatctg
    16021 gcaacagaga gccgcgcctg gctgatacgt tcccgtcgcc gcctgacggt tttctctgcc
    16081 acaggcggcg tggcagcact gctgctcatc accggctggc atcactatta caacggtaac
    16141 tatcagtccg gcatcaccgt gcttaagcag gccaaagcct ttatggacgt gccgcctccg
    16201 cagggggaag atgactttgg caacctgcaa ctgccgctcc tgaacccggt acgcgatgcc
    16261 acactggcct atggcgactg gggcgaccgc agccgtctgg ccgatatggg actgtaccag
    16321 ggacgacgta tcgggcctta tgtggaacag acctatctgc aactgctgga gcaacgttac
    16381 ctgccctcgc tgtttaacgg gctggtcaaa gcgatgaacg ccgcgccgcc ggagagtgaa
    16441 gaaaaactcg cggtgctgcg cgtgatgcga atgctggagg acaaaagcgg acgtaacaat
    16501 caggtggtga agcagtatat ggcaaaacgc tggagcgaaa agttccacgg tcagcgcgat
    16561 atccaggcac aactgatgtc ccatcttgac tacgcgctgg ctcatactga ctggcacgca
    16621 gagcgtcagg cgggcgacgg tgacgccatc agccgctgga cgccatatga caagcccgtg
    16681 gtatcagcac agaaagaact gagcaaactg cctgtctacc agcgggttta ccagagcctg
    16741 aaaacgcggg cgctgggcgt tcttcctgcc gacctcaatc tgcgtgacca ggtagggcca
    16801 acctttgacc aggtgtttac atctgccgat gacaacaaac tggttgttcc acagtttctt
    16861 acccgttacg gcctgcaaag ctattttgta aaacagcgcg atgaactggt tgaactgacg
    16921 gcgatggatt cctgggtact taaccttacc cgcagcgtga aatacagtga cgccgaccgc
    16981 gcggaaatcc agcgccagtt gaccgagcag tatatcagcg actacaccgc cacctggcgg
    17041 gccgggatgg acaatctgaa tatccgcaat tttgagtcca tcggacaact gaccggggcg
    17101 ctggagcagg ttatcagcgg cgaccagcct ttgcagcggg cgctgaccgt gctgcgtgac
    17161 aacacacagc caggcgtctt ttctgaaaaa ctctctgcca aagaacggga ggaagccctg
    17221 gcagagccgg attaccagtt actcacccgc ctcgggcatg aattcgcccc ggaaaacagt
    17281 accctggcag tacagaaaga caaagaaagc acgatgcagg ccgtgtatca gcaactcacc
    17341 gagttgcacc gctacctgct ggcaatccag aacgcgcctg taccagggaa atcggcgctg
    17401 aaagccgtgc agttacggct tgatcagaac agcagcgatc cgatattcgc cacccgccag
    17461 atggcaaaaa cgctgcctgc tccgctcaac cgctgggttg gcagactgac tgaccaggcc
    17521 tggcatgtgg tgatggtgga ggctgttcat tatatggaag tggactggcg cgacagcgtg
    17581 gtgaaaccgt ttaacgagca actggcaaat aactatccgt ttaatccgcg ttctgcacag
    17641 gatgcctcac tggatgcctt cgaacgcttc tttaaaccgg atggcatact ggataccttc
    17701 taccagcaga acctgaagct gtttatcgat aatgacctga gtctggagga tggcgataac
    17761 aacgtcatta ttcgcgaaga tattattgcg caactggaaa ctgcgcagaa aatccgtgac
    17821 atcttcttca gcaaacagaa cggtctggga acatcctttg ccgtggaaac ggtatcgctt
    17881 tcaggcaata aacgccgcag tgtactgaac cttgacggtc agttagtcga ttacagccag
    17941 ggccgtaact ataccgccca tctggtctgg cctaacaaca tgcgcgaagg caacgaaagt
    18001 aagctgacgc tcatcggcac cagcggcaac gcgccgcgca gtatcagctt cagcgggccg
    18061 tgggcgcagt tccgcctgtt cggggccgga caactgaccg gagtacagga tggcaacttt
    18121 accgtgcgct ttagcgtgga cggtggcgcg atgacctacc gtgtgcatac cgacacggaa
    18181 gataacccgt tcagcggtgg gttgttcagc cagtttggtc tgtcagacac actgtactga
    18241 ggaggcaggg atgaatagta acgtactgac acaaactatc gttaccggca gtgacccgcg
    18301 cgggctgccg gaattcagcg ccatccgcga ggaaataaac aaagccagcc acccgtcaca
    18361 gcctgagctg aactggaaac tggtggagtc gctggcgctg gcgattttta aagccaacgg
    18421 tgtggattta cacaccgcca cctactatac gcttgcccgg acacggacac agggactggc
    18481 gggattctgc gaaggtgcgg aactgctggc ggcaatggta agccacgact gggataagtt
    18541 ctggccgcag ggcggcccgg cgcgtactga aatgctggac tggtttaact cccgaaccgg
    18601 caatattctg cgtcagcaaa tctcctttgc ggaatccgac ctaccactga tctaccgcac
    18661 agagcgggca ttgcagctta tctgcgataa gctccagcag gtggaactga agcgcgttcc
    18721 gcgcgtggag aatctgctct attttatgca gaacacgcgt aaacggcttg aaccccagct
    18781 gaagagtaac actgagaacg ccgcacagac cacggtcaga acgctgattt atgccccgga
    18841 aacacaggca tcttccacac cagaagcggt agtgcctccc ctgcccggcc tgcctgagat
    18901 gaaagtggaa gtgcgcagtc tgacagagaa tcccccacag gccagtgtga taaagcaagg
    18961 cagtacggta agagggttta tcgcagggat cgcctgttca gtggctgtcg cctcagcatt
    19021 gtggtggtgg caggtctatc cggtgcagca gcaactgtta caggttaacg acaccgctca
    19081 gggcgcagca acggtgtgga tggcctcacc tgaactcgaa aactatgagc gcaggctgca
    19141 acaacttctt gatacctccc cggtacagcc gctggaaacc gggatgcaga tgatgcgtgt
    19201 tgccgacagt cgctggccgg aaagcctgca acagcaacag gcctcgacac aatggaatga
    19261 ggcactcaaa acccgcgcac agagtagccc gcagttgcgt ggctggttgc agacccgcca
    19321 ggacttacat gcttttgcag atctagtgat gcagcgcgag aaagagggac taaccctttc
    19381 ctatatcaaa aatgtcatct ggcaggcgga gcggggactg gggcaggaaa cacccgttga
    19441 gtctctgttg acgcagtacc atgatgcccg tgcgcagaag cagaatacag atacgctgga
    19501 aaaacaaatt aatgagcgac tcgaaggcgt gttaagccgc tggctgctgc tgaagaataa
    19561 cgtcatgcca gaggcggcaa ccggtaccac ggccgaaaaa taattattta attaaaggag
    19621 attatcatgg caaatataag ttatttatct ttgagtggag agacacaagg gctaatttca
    19681 gcaggttgct caacgcttga ttctgtcgga aacaaagcgc agccagaaca taaagatcag
    19741 atcatggttt atgcgttaat gcattccatc tcaagaagtc aaaatgtcaa tcatcatgaa
    19801 ttaattatca ccaaaccggt tgataaatcc tcaccattgc tggcaaaagc acttagtgac
    19861 aatgaaaaaa tggcaatatg tgaatttata ctctaccgaa ccagtaaagc gggtatttac
    19921 cagccttatt ataaaataaa tctgtcaaaa gcccgaatat caagtattga ttttgtcaca
    19981 ccgcatgctg tcctggaaaa agaactagaa ccacaggaga gaattgcctt tatttatgaa
    20041 gatatttcct gggagcatac gttagccggt acgaatgcaa tgagtaaatg gcaggatcgt
    20101 gtacagtaac ttactgtaat aacataataa attactattg cggagagata aaatggctgg
    20161 tttaaggaat aacaacaaca cacagaatgc acagtgggct gattatgtgg gtgacattct
    20221 tcggggggct cagccgataa accagctagt acctcagcac ccttatctta acgacgtccc
    20281 actcattgat gagttaaggc atcagaacac tcatcatgtt attctcttga cacttgatgt
    20341 tgcaaaaaaa atactcagcc caataacatc atttgattac attcatttta tcactacaca
    20401 tccttcaggt ataaaagata cactcgcctg gttagttaac gcgagtaaat taatgactga
    20461 gtttgatgac aatgggaaaa ttatatttaa ccttaatgcc ttaaagtata caaaagcaag
    20521 ttactttgaa atacttggag agaaatatat aaaaatcaca acatccagcc cctggctttt
    20581 agaaaagctc ggtaaatata ttttctcttc acgagcgcct caagttctgg aactcgctat
    20641 tggctggcga ggagctttat ctgaatcaat aaaaggagtt aagttctgta tctggttttc
    20701 agtagcctgg agaaccatag aatttattat gagttcagag cgagatctgg ttaattttct
    20761 tggcgatttt tcaatggatg tggccaaagc agttatagcc ggtggtgttg caaccgctat
    20821 tggaagcctg gcttcttttg cctgtgttag ctttggcttt ccagtaattc ttgtcggagg
    20881 agcaatttta ctgacaggga tagtgtgtac agttgtttta aatgaaatcg atgctcaatg
    20941 ccatttatca gaaaaattaa aatatgcaat tagagatgga ctaaaacggc aacaggaact
    21001 tgataaatgg aaaagggaaa acatgactcc atttatgtat gttcttaaca ctccacccgt
    21061 gatatgagga ttaaccatgc gtttacatgt taaattaaaa gagtttcttt cgatgttttt
    21121 tatggccata ttatttttcc ctgcattcaa tgcttctttg ttttttaccg gagttaaacc
    21181 tctatattca ataataaaat gctccacgga aatattttat gattggagaa tgttgattct
    21241 gtgcttcggt ttcacgtcgt tttccttttt aaatattcat gtaattttac ttacgataat
    21301 aaaatcattc ttaatcaaaa aaacgaaggt agtcaatttc gccacagaca taacaataca
    21361 gctaactctt atatttttac taatagctat cgtaatagca cctttaatag ctccttttgt
    21421 cacaggatat gtcaatgcta attatcaccc ctgcggtaat aata
//
LOCUS       DAJOWB010000079        21418 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJOWB010000079 DAJOWB010000000
VERSION     DAJOWB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.079"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            9..1523
                     /gene="caiT"
                     /locus_tag="OHO85_003152"
     CDS             9..1523
                     /gene="caiT"
                     /locus_tag="OHO85_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCQ2672495.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1554..2696
                     /gene="caiA"
                     /locus_tag="OHO85_003153"
     CDS             1554..2696
                     /gene="caiA"
                     /locus_tag="OHO85_003153"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCQ2672496.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2825..4042
                     /gene="caiB"
                     /locus_tag="OHO85_003154"
     CDS             2825..4042
                     /gene="caiB"
                     /locus_tag="OHO85_003154"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCQ2672497.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKARETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLSTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSYGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4116..5669
                     /gene="caiC"
                     /locus_tag="OHO85_003155"
     CDS             4116..5669
                     /gene="caiC"
                     /locus_tag="OHO85_003155"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCQ2672498.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLLTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5778..6563
                     /gene="caiD"
                     /locus_tag="OHO85_003156"
     CDS             5778..6563
                     /gene="caiD"
                     /locus_tag="OHO85_003156"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCQ2672499.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGTEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAVEGPLAFAEKRDPVWKGR"
     gene            6569..7159
                     /gene="caiE"
                     /locus_tag="OHO85_003157"
     CDS             6569..7159
                     /gene="caiE"
                     /locus_tag="OHO85_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCQ2672500.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7245..7640)
                     /gene="caiF"
                     /locus_tag="OHO85_003158"
     CDS             complement(7245..7640)
                     /gene="caiF"
                     /locus_tag="OHO85_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCQ2672501.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7901..11122)
                     /gene="carB"
                     /locus_tag="OHO85_003159"
     CDS             complement(7901..11122)
                     /gene="carB"
                     /locus_tag="OHO85_003159"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCQ2672502.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11140..12288)
                     /gene="carA"
                     /locus_tag="OHO85_003160"
     CDS             complement(11140..12288)
                     /gene="carA"
                     /locus_tag="OHO85_003160"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCQ2672503.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(12744..13565)
                     /gene="dapB"
                     /locus_tag="OHO85_003161"
     CDS             complement(12744..13565)
                     /gene="dapB"
                     /locus_tag="OHO85_003161"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCQ2672504.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            complement(13732..14646)
                     /gene="rihC"
                     /locus_tag="OHO85_003162"
     CDS             complement(13732..14646)
                     /gene="rihC"
                     /locus_tag="OHO85_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCQ2672505.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            complement(14712..15662)
                     /gene="ispH"
                     /locus_tag="OHO85_003163"
                     /gene_synonym="lytB"
     CDS             complement(14712..15662)
                     /gene="ispH"
                     /locus_tag="OHO85_003163"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCQ2672506.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKSAFLIDDAKDIQEEWVKEVKCIGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(15664..16113)
                     /gene="fkpB"
                     /locus_tag="OHO85_003164"
     CDS             complement(15664..16113)
                     /gene="fkpB"
                     /locus_tag="OHO85_003164"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCQ2672507.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(16238..16732)
                     /gene="lspA"
                     /locus_tag="OHO85_003165"
     CDS             complement(16238..16732)
                     /gene="lspA"
                     /locus_tag="OHO85_003165"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCQ2672508.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(16732..19548)
                     /gene="ileS"
                     /locus_tag="OHO85_003166"
     CDS             complement(16732..19548)
                     /gene="ileS"
                     /locus_tag="OHO85_003166"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCQ2672509.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(19591..20532)
                     /gene="ribF"
                     /locus_tag="OHO85_003167"
     CDS             complement(19591..20532)
                     /gene="ribF"
                     /locus_tag="OHO85_003167"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCQ2672510.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(20540..20758)
                     /locus_tag="OHO85_003168"
     CDS             complement(20540..20758)
                     /locus_tag="OHO85_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCQ2672511.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            20861..21124
                     /gene="rpsT"
                     /locus_tag="OHO85_003169"
     CDS             20861..21124
                     /gene="rpsT"
                     /locus_tag="OHO85_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCQ2672512.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         5068 a         5779 c         5524 g         5047 t
ORIGIN      
        1 attaactcat gaagaatgaa aagagaaaaa cgggaataga accgaaggtt ttctttccgc
       61 cgttaataat cgtcggcata ctttgttggc ttacagtcag agatctggat gcagcgaatg
      121 tcgttattaa tgctgtattc agttacgtca ccaatgtatg gggatgggca tttgaatggt
      181 atatggtggt gatgcttttc ggttggttct ggctggtgtt tggcccgtat gccaaaaagc
      241 gtttaggtaa cgaaccacct gaatttagta ccgccagttg gatctttatg atgttcgcct
      301 cctgtacgtc tgctgccgta ctgttctggg gatcgattga gatctactac tacatctcca
      361 ccccaccgtt tggcttagaa ccgaactcga caggagcgaa agagttgggg ctggcttaca
      421 gcttgttcca ctggggacct ctgccgtggg ccacttacag cttcctttca gtcgccttcg
      481 cttacttctt ctttgtccgc aaaatggaag tgattcgccc cagctcgaca ctggtgccgc
      541 tggtaggtga aaaacacgcc aaagggttgt tcggcactat cgtcgacaac ttctatctcg
      601 tcgccttgat cttcgcgatg ggtaccagtc tgggccttgc cacgccgctg gtgaccgagt
      661 gtatgcaatg gttgtttggc attccgcata ccctgcaact ggacgctatc atcattacct
      721 gctggattat cctcaacgcc atttgcgtcg cttgcggtct gcaaaaaggg gtacgtatcg
      781 ccagtgacgt gcgtagttac ctgagcttcc tgatgctggg ttgggtgttc attgtcagcg
      841 gtgccagctt catcatgaac tacttcaccg attcggtggg gatgttgctg atgtatctgc
      901 cgcgcatgtt gttctatacc gatcccatcg ctaaaggcgg cttcccgcag ggctggaccg
      961 tgttctactg ggcatggtgg gtgatttatg ctatccagat gagtatcttc ctcgcccgca
     1021 tctcccgtgg tcgtactgtg cgtgaactgt gcttcggcat ggtgctgggg ctgacagcgt
     1081 caacctggat cctgtggact gtactcggta gtaacactct gctgttgata gataaaaaca
     1141 tcatcaacat tccaaatctg atcgaacagt acggtgtggc gcgcgccatc attgaaacct
     1201 gggccgctct gccactcagc accgccacca tgtggggctt cttcatcctc tgctttattg
     1261 ccaccgttac gctggttaac gcctgctctt ataccctggc gatgtccact tgccgcgaag
     1321 tacgcgatgg tgaagaacca cctctgctgg tgcgtatcgg ttggtcaatt ctggttggca
     1381 ttatcggtat tgttctgctg gcgctcggcg gcctgaaacc gattcaaacc gccattatcg
     1441 ccggaggatg cccgctgttc ttcgtcaaca ttatggtgac gctctccttt attaaagacg
     1501 cgaaacagaa ctggaaagat taattaaccc ccaaaatatc aagaggttga aagatggatt
     1561 ttaatttaaa tgatgagcag gaactgtttg tcgccggtat ccgcgaactg atggccagcg
     1621 aaaactggga ggcctatttt gccgagtgcg accgtgacag cgtctacccg gaacgttttg
     1681 tcaaagcact ggcggatatg ggtatcgaca gtctgctgat ccctgaagag cacggtggtc
     1741 tggacgcggg gtttgttact ctcgccgccg tgtggatgga gctgggacgt ctgggggcac
     1801 caacctatgt gctgtaccag ttgccgggcg ggttcaacac cttcctgcgc gaaggcacac
     1861 aagagcagat cgacaagatt atggctttcc gcggcaccgg taagcagatg tggaactcag
     1921 cgattaccga accgggcgcg ggctccgacg tgggtagcct gaaaacgact tatacccgta
     1981 gaaatggtaa gatttatctt aatggtagta agtgttttat taccagcagc gcctacaccc
     2041 cgtacatcgt ggtgatggcg cgcgacgggg cttctccgga caaacctgtc tacaccgaat
     2101 ggtttgttga tatgagcaaa ccgggcatca aagtgaccaa acttgaaaag ctcggtctgc
     2161 gtatggatag ctgctgtgaa atcacctttg acgacgtgga actggacgag aaagacatgt
     2221 tcggtcggga aggtaacggc tttaaccgcg tcaaagaaga gttcgatcat gaacgtttcc
     2281 tggtagctct caccaactac ggtacggcga tgtgcgcctt tgaagatgcg gcgcgctacg
     2341 ccaaccagcg cgtgcagttt ggcgaggcta ttggccgttt ccagttgatt caggaaaaat
     2401 tcgcccacat ggcgatcaaa ttaaactcca tgaaaaacat gctgtatgaa gcagcgtgga
     2461 aagcagacaa cggcaccatc acctctggcg atgcagcgat gtgcaaatac ttctgcgcca
     2521 atgcggcatt tgaagtggtg gatagcgcaa tgcaggtgct gggcggtgtc gggattgcgg
     2581 gcaaccaccg catcagccgc ttctggcgtg acctgcgtgt agaccgcgtc tccgggggat
     2641 ctgacgaaat gcagatcctg acgctgggtc gcgcggtgct gaagcaatac cgctaagtcc
     2701 gtgcgctgcc tgatgcgacg ctaatgcgtc ttatcaggcc tacgggagca cgaatgtagg
     2761 ccggataaag cgtttacgcc gcatccggca gtcatgcgct acataaattt ttcaggagct
     2821 aataatggat catctaccca tgccgaaatt cgggccgttg gccggattgc gcgttgtctt
     2881 ctccggtatc gaaatcgccg ggccgttcgc cgggcaaatg ttcgcagaat ggggtgcgga
     2941 agttatctgg atcgagaacg tcgcctgggc cgacaccatt cgcgttcaac cgaactaccc
     3001 gcagctctcc cgccgcaatt tgcacgcgct gtcgttaaat attttcaaag atgaaggccg
     3061 cgaagcgttt ctgaaattaa tggaaaccac cgatatcttc atcgaagcca gcaaaggtcc
     3121 ggcctttgcc cgtcgtggca ttaccgatga agtactgtgg cagcacaacc cgaaactggt
     3181 tatcgctcac ctgtccggtt ttggtcagta cggcaccgag gagtacacca atcttccggc
     3241 ctataacacc atcgcccagg ccttcagtgg ttacctgatt cagaacggtg atgttgacca
     3301 gccaatgcct gccttcccgt ataccgccga ttacttttct ggcctgaccg ccaccacggc
     3361 ggcgctggca gcactgcata aagcgcgtga aaccggtaaa ggcgaaagta tcgacatcgc
     3421 catgtatgaa gtgatgctgc gtatgggcca gtacttcatg atggattact tcaacggcgg
     3481 cgaaatgtgc ccgcgcatga gcaaaggtaa agatccctac tacgccggtt gcggtctgta
     3541 taaatgtgcc gacggctaca tcgtgatgga actggtgggc attacccaaa ttgaagagtg
     3601 ctttaaagat attggcctcg cacatctact cagtacgccg gaaatcccgg aaggcactca
     3661 gcttatccac cgtatcgaat gcccttacgg cccactggta gaagaaaaac tcgatgcctg
     3721 gctggcggca cataccatcg cagaagttaa agaacgcttt gccgaactga atatcgcctg
     3781 tgccaaagta ctgaccgtac cggaactgga aagcaatcca cagtatgttg cccgtgaatc
     3841 aatcactcag tggcaaacga tggatggtcg cacctgcaaa gggccgaaca tcatgccgaa
     3901 attcaaaaat aaccccggac aaatctggcg cggaatgccc tcatatggca tggacacggc
     3961 tgccattttg aaaaatatcg gctacagcga aaacgacatt caggagttgg tcagcaaagg
     4021 tctggccaaa gttgaggact aaacgcgtgg cgtcagtcgg cttgcctgac tagcgcccgc
     4081 ccaataacaa acgaacaaaa atggatagag gtgcaatgga tatcattggc ggacaacatc
     4141 tacgtcaaat gtgggacgat ctggcggacg tttacggtca taaaacggcg ctgatttgtg
     4201 aatccagcgg cggagtcgtt aaccggtata gttatcttga gttaaatcag gagattaacc
     4261 gcacggcaaa cctgttttat acgctgggga ttcgcaaagg cgacaaggtt gcactacatc
     4321 tcgacaactg cccggaattt atcttttgct ggttcgggct ggcaaaaatt ggcgcgatta
     4381 tggtgccgat taacgcccgc ctgttacgcg aggaaagcgc gtggatcctg caaaatagcc
     4441 aggcgtgcct gctggtgacc agtgcgcaat tctatcctat gtatcaacag attcagcagg
     4501 aagatgccac tcaattgcgg cacatttgcc tgacagatgt ggcacttccc gctgatgatg
     4561 gcgtgagttc gtttactcaa ctgaaaaatc aacaacctgc caccttgtgc tatgcaccgc
     4621 cgctattgac tgacgatacg gcggaaattc tcttcacctc cggcaccacc tcccgaccga
     4681 aaggtgtggt gattacccat tacaacctgc gcttcgctgg atattactcc gcctggcagt
     4741 gtgcactgcg tgacgatgac gtctacctga cggtaatgcc tgcgtttcat atcgattgcc
     4801 agtgtactgc ggcgatggcg gcgttttctg ccggggccac ctttgtgctg gtcgagaaat
     4861 acagcgcccg cgccttctgg ggacaggtgc agaagtaccg cgccaccatt accgaatgta
     4921 ttccgatgat gatccgtacg ttgatggtgc agccgccttc agcgaacgat cggcaacacc
     4981 gcctgcggga agtgatgttt tatctcaact tgtcggagca ggaaaaagat gcgttttgtg
     5041 aacgcttcgg cgttcgcttg ctgacgtctt atgggatgac ggaaaccatt gtgggcatta
     5101 tcggcgatcg ccctggcgat aaacgacgct ggccgtcgat tggtcgggcg gggttttgct
     5161 acgaagcgga gatccgcgac gatcacaatc gcccgctccc ggcaggtgag atcggtgaaa
     5221 tctgtattaa aggcgtacca gggaaaacca tcttcaaaga gtattttctc aacccgaaag
     5281 ccactgcgaa agtgctggaa gccgacggct ggctacatac cggcgatacc ggatactgcg
     5341 acgaagaggg ctttttttat ttcgtcgatc gccgctgcaa tatgatcaaa cgcggtggcg
     5401 agaatgtctc ctgcgtggag ctggaaaata tcatcgccac ccatccaaaa attcaggata
     5461 tcgtggttgt gggtattaaa gattcgattc gcgatgaagc catcaaagca tttgtggtgc
     5521 tgaatgaagg tgaaacattg agcgaagagg aatttttccg cttctgcgaa caaaatatgg
     5581 cgaaatttaa agtgccctct tatctggaga tcagaaaaga tctgccacgt aattgctcgg
     5641 ggaaaataat tagaaagaat ctgaaataaa tgaaacagca gggaactacc ctgccagcta
     5701 ataatcacgc tataaaacaa tatgcatttt ttgcagggat gttgtcgtcc ctgaaaaagc
     5761 aaaaatggag aaaaggaatg agtgaatcat tacatctgac ccgcaatgga tcaattctgg
     5821 aaattaccct tgatcgtcca aaagcgaatg ctattgatgc aaaaaccagc tttgaaatgg
     5881 gcgaagtatt tctaaatttc cgtgacgatc cgcaattacg tgtcgccatt attaccggtg
     5941 ccggagagaa gttcttttcc gcgggctggg atttaaaagc ggcagcagaa ggcgaagcac
     6001 cggatgctga ctttggcccg ggtggatttg cgggattaac cgaaattttc aatctcgaca
     6061 aaccggttat cgcagctgtg aacggctatg cctttggcgg cggtttcgaa ctggcgctgg
     6121 cggcagattt tattgtttgt gccgataacg ccagcttcgc cctgccggaa gccaagctgg
     6181 gcatcgttcc tgacagcggc ggcgtgctgc gtctaccgaa gatcctgccg cctgccatcg
     6241 tcaatgaaat ggtgatgacc ggcagacgaa tgggcacaga agaggcgctg cgttggggga
     6301 tagtcaaccg cgtggttagc caggcggaac tgatggataa cgcccgcgaa ctggcgcagc
     6361 agctggttaa cagcgccccg ctggcgattg cggcgctgaa agagatctac cgcaccacca
     6421 gcgaaatgcc ggtagaagaa gcgtatcgct atattcgcag cggcgtgttg aaacactatc
     6481 cgtcggttct gcattcggaa gatgccgttg aagggccgct ggcgtttgcc gagaagcgcg
     6541 atccggtgtg gaaaggacgt taacaaccgt gagctattac gcctttgagg ggttaattcc
     6601 ggtggttcac ccgacggcgt ttgtccatcc cagtgccgtc ttgattggcg atgtgattgt
     6661 gggagccggt gtctacatcg gcccactcgc ctcactgcgt ggtgactacg ggcggttgat
     6721 cgtgcaagcg ggagccaata ttcaggatgg ctgcattatg catggctact gcgacactga
     6781 caccatcgtt ggggaaaacg gccatatcgg gcacggagcg atcctgcatg gttgtgtgat
     6841 tggtcgcgat gcattggtcg ggatgaacag cgtgattatg gatggcgcgg tcattggcga
     6901 agagagcatt gttgccgcca tgagctttgt caaagcaggc tttcacggcg agaaacgcca
     6961 gttgttgatg ggtacgccag cccgcgctgt acgcagtgtt agtgacgacg agttacactg
     7021 gaaacggttg aataccaaag agtatcagga tcttgtcggg cgctgtcatg catcgttaca
     7081 tgaaacacag ccactgaggc aaatggagga aaatcgcccc cgtttgcagg ggacgacgga
     7141 tgtgacgcct aaacggtgat cttgccggat gcgacctggt tgctggataa gatgctacaa
     7201 catcgcatct ggcaagcagg acgcccggta tcaacgagat aaaattaacg acgcatactc
     7261 tttgacaaca tcatctgcca cttctgctcg cggttaagtt ccgtgagcgg aacggcagga
     7321 atacgcggcg tctttcttac gccgactaat ttatcccggc tgttattgcg caggcgcgcg
     7381 taaatttgct catcgatatc gaccactttt accagtcgct ggcactggca tccccgtcct
     7441 tccagcttat tagggatcat cttaacttcg cagcttattt ctgcgacttc cgacagaata
     7501 taagtcaggg tattaaccgc cttgctgtgt tcaatatcga aatgaataga gatctctctt
     7561 gctatcaccc accgattttc agccatcatc cattcggcga ttaacaagta gagtggtttt
     7621 tcaacatatc cttcacacat acgaactcca gtttcacaaa ttaaaagtct ggctatgact
     7681 cgttttgaag caattaaaca aggccatatt aaaattgtgg atatatcgat agtatgcaac
     7741 ttcaccgtcg ataagtgtga gctaaatcct gcttatgtca tcaaaaatat cgattcccca
     7801 aaagatttac ttaatgggca aaaaaaacgg tcctcatcag agaaccgtat tacttattcg
     7861 atcaaattag cggatgaaaa atatccgcca tgacacgcta ttatttgatc tgcgcgtgca
     7921 tttcctgcac cgaaattact ttttcggtcg cgtcggcatt cagcgccatc gcggtagcga
     7981 aaccaccgtt cagggtggtg tcataatgca ctttatattg cagcgcactg cgacggatca
     8041 cgcgggagtc ttcaatcgcg cgacggcctg aagtggtgtt gatgatgtag gtatattcgc
     8101 cattcttgat gcggtcctga atgtgcggac ggccttcatg caccttgttc accagacgcg
     8161 gattaatgcc cgcttcgccc agcacaatcg ccgtgccgtg ggtcgcatcc agctcgaagc
     8221 cctgtttcag cagttttgcc gccaggtcca ccacgcgttc tttatcgcct tcgcgcacgg
     8281 aaagcaacgc acgaccgtgt ttcttcatgg tggagttgct gcccagctgc gctttggcaa
     8341 acgcttcagc gaaggtgcgg cccacgccca tcacttcccc ggtggagcgc atttctggcc
     8401 ctaacagcgg gtcaacaccc gggaatttat tgaacggcag caccacttct ttcaccgagt
     8461 agtacggcgg gataacttct ttggttacgc cctgctcagc cagcgatttg ccagccatca
     8521 cacgcgccgc cacttttgcc agcggtacgc cggtggcttt ggagacgaac ggaacggtac
     8581 gcgccgcacg cgggttaact tcaatcaggt agacttcgtt gtttttcacc gcaaactgca
     8641 cgttcatcag accgcgcacc tgcaattcga aggccagttt ctgcacctgc tggcgcatca
     8701 catcctgaat ttcctgactt aaggtgtagg ctggcagaga acatgcggag tcaccggagt
     8761 gcacgcccgc ctgctcgatg tgctccatga tgccgccaat cagcaccatt tcgccgtcgc
     8821 agatggcatc cacgtcaact tctaccgcgt catcaaggaa gtgatccagc aacactggcg
     8881 catcgttaga cacgctgacc gccgtctgga agtagcgacg caggtcagct tcgtcataga
     8941 cgatttccat cgcccgaccg ccgagaacgt aagacggacg taccaccagc gggtagccaa
     9001 tctctttcgc cttctctacc gccatttcaa tagcggtaac ggtggcgttc gccggttgtt
     9061 tcagtttcag acgctcaacc gcatgctgga agcgttcacg gtcttctgca cggtcgatag
     9121 catccgggct ggtgccgata accggtacgc cagcagcttc cagcgcgcgc gccagtttca
     9181 gcggggtctg accgccgtac tgaacgataa cgcctttcgg cttctcaata cgcacgattt
     9241 ctagcacatc ttccagagtt accggctcga agtagaggcg atcggaagtg tcgtagtcgg
     9301 tggagacggt ttccgggtta cagttaacca taatggtttc gtaaccgtct tcgcgcagcg
     9361 ccagcgaggc gtgtacacag cagtagtcga attcgatacc ctgaccgata cggttcgggc
     9421 cgccgccgag aaccatgatt ttttcacggt cggtagacgg attcgcttcg cactcttctt
     9481 cataagtgga gtacatgtaa gcggtgtcgg tggcgaactc tgccgcacag gtatccacgc
     9541 gcttataaac cgggtgcagg tcatactggt cgcgcagctt gcggatttcc gcttcgcgca
     9601 cgcccgccag ttttgccaga cgcgcatcgg caaagccttt gcgtttcagc tggcgcagga
     9661 attcagcgtt caggccagtg atgcccactt ccgctacttt ctcttccaga cgcaccagtt
     9721 cttcaatctg taccaggaac cagcggtcaa tattggtcag gttgaacacg ccgtccacag
     9781 acaggcccgc acggaaggca tcggcgatgt accagatacg ctctgcgcca gcatctttca
     9841 gttcgcgacg gattttggtt aacgcttccg ggtcatcgag gctcactttc gggtcgaatc
     9901 cagtcgcacc gacttccagg ccgcgcagcg ctttttgcag ggattcctgc tgcgtgcgac
     9961 caatcgccat cacttcgcca accgatttca tctgagtggt cagacggtcg ttagcaccag
    10021 cgaatttttc gaagttgaag cgaggaattt tagtaaccac atagtcgatg gacggctcga
    10081 aggaggccgg agtacgtccg ccagtgatgt cgttcatcag ttcgtcgagg gtgtaaccca
    10141 ccgccagttt cgccgccact ttagcaatcg ggaaaccggt cgctttcgac gccagcgccg
    10201 aagaacggga cacgcgtggg ttcatttcga taacaatcag gcgaccgttt ttcgggttca
    10261 ccgcaaactg gacattggaa ccaccggttt caacgccgat ttcacgcagc accgccatcg
    10321 aggcgttacg catgatttga tattctttgt cggtcagcgt ttgggctggc gcgacagtga
    10381 tggagtcacc ggtatggatg cccatcgcat caaagttttc gatagagcag acgatgatgc
    10441 agttgtcgtt tttatcacgc accacttcca tctcgtactc tttccagccg atcagcgact
    10501 catcaatcag cagctctttg gttggggaga gatccagacc gcgggcgcaa atttcttcaa
    10561 actcttcgcg gttataagcg ataccgccgc cgctaccgcc catggtaaag gatgggcgaa
    10621 taatgcacgg gaagcccacg tcagcggcaa ccgccagcgc ttcttccatc gtgtgtgcga
    10681 taccggaacg cgcggtttcc agaccaattt tcttcatcgc tacgtcgaaa cgacggcggt
    10741 cttctgcttt atcaatcgca tcggcggtcg caccaatcat agtcacgccg aactcttcca
    10801 acacgccctg acgctccagc tccagcgcgc agttcagcgc cgtctgaccg cccatcgttg
    10861 gcagcaccgc gtccgggcgc tctttttcaa taatcttgcg tactacttcc cagtgaatcg
    10921 gctcgatgta ggtcgcatcg gccatttccg ggtcggtcat gatggtcgcc gggttggagt
    10981 tcaccagaat gacgcggtaa ccctcttcgc gcagggcttt acatgcttgc gcgccagagt
    11041 agtcaaactc acacgcctga ccgataacaa tcgggcccgc acccagaatc aggatacttt
    11101 ttatatctgt acgttttggc atggctcttt tactcctgat tacttagcgg ttttacggta
    11161 ctgctcaatt aactcgataa agtggtcgaa caacggcgct gcgtcgtgtg gcccagggct
    11221 ggcttccggg tgaccctgga agctgaacgc cggtttatca gtgcgatgaa tgccctgtaa
    11281 cgtaccgtcg aacagggatt tatgcgtgac acgcaggttt gcaggtaatg ttgcttcatc
    11341 caccgcaaaa ccgtggttct gggcggtgat catcaccacg tttttctcca catctttaac
    11401 cggatggttg ccgccgtggt gaccaaattt cattttgaca gtcttcgcac cgctcgccag
    11461 cgccagcagc tgatgaccga ggcagatgcc gaataccgga atatcggttt cgaggaattt
    11521 ctggatggcg gtaatggcgt aatcgcacgg ggccgggtcg ccaggaccgt tggagaggaa
    11581 gatgccgtct ggattcattt tcagcacatc ttccgcagaa gtttgcgccg gaacgatggt
    11641 caggcgacag cctctatcca ccagcatccg caggatgttg cgcttggcac caaaatcata
    11701 agccacgacg tggaacggca gctcgtcttc ttttttcgct tctggcaggc cgccggtcaa
    11761 cgtccagctc ccttgtgtcc agctataggc ttctgcggtg gtcacttctt ttgccagatc
    11821 catgccattc agacctggga acgcgcgggc tttttctaac gccaacgccg catccgggtt
    11881 atcgcctgcg ataatgcagc cattctgtgc gcctttctcg cgcagtaaac gcgtcagctt
    11941 acgggtatcg atatcggcaa tcgccacgat gttatggcgc ttcaggtaag aagaaaggtc
    12001 ttcggtatta cggaagttgc tggcaatcag cggcagatcg cgaatcacca gaccttgtgc
    12061 atgtacctga gaagattctt catcggcgtc attagtgccg acattgccaa tatggggata
    12121 agtaagagta acgatttgac gagaatagga aggatcagtg aggatttctt gataaccggt
    12181 cattgaagta ttgaaaacga cttccccaac cgccgaacct gttgccccta tggcccgacc
    12241 gtgaaactgg gttccgtctt ccagaaccaa tagcgctgac ttaatcaaaa caccctccag
    12301 agaatattca ctcactttat ttgcatatta attcacaatg atgacgtaaa tcaatgcaaa
    12361 tctgcttacc gacgaatttc tggcaaacgg cggcattctg gagatatggg cgctaaaagt
    12421 caacttaatg gcgggatttt ttatattatt ttgtggctca tgcatgtttt acaggcatta
    12481 agcgacaaaa agatcatata acctgttctg gaaaacgctt gcgcagcaag aagattgcat
    12541 gaaataaaag cgagcaggga aaagtggacc aaatggtcaa aatttacatt aaaacaacaa
    12601 aaaccgaaga caaaacatgt aatttttggc acttatagac aatttaaaaa cataacacta
    12661 aaaataaaag ggccaaaaat taaagccctt tataatcaat aaattaaatg tgttattctt
    12721 gcaccataac aaatattttg tggttacaaa ctattgagat caagcacatc tcgcatatca
    12781 aaaagaccac tttccttacc actcaaccac aaagccgatc ttaccgcgcc gttagcaaat
    12841 gtcatgcggc tggacgcctt atgggtgatc tccagacgct cgccaatatc ggcaaacatc
    12901 gcggtatgtt cgccaacgat gtcacctgca cgcacggtgg caaaaccaat ggtgccaggc
    12961 acacgttcac cggtgtggcc ttcacgactg tagaccgcgc aatctttcag atctttatca
    13021 agggcgtggg cgatcgcctc tcccattgcc agtgcggtgc ctgacggcgc atcaacttta
    13081 tgtctatgat gtgcttcaat aatttcgata tcggtgtagt cacccatcac tttggctgct
    13141 ttctccagca gcttaagcat gacgttaacg ccaacgctaa aattggcggc aaagacaatc
    13201 gcaatatcgg cggcggcgtc acgaatcgcc tgtttaccgg cttcgtcaaa ccccgtagtg
    13261 ccgatcacca tccctttgcc atgctggcga caaaaagcga gatggttcag cgtaccttcc
    13321 ggacgggtaa aatcgataaa cacatcaaaa tcatctttta ccgcatcgag gctgctttgc
    13381 acggtaacgc ctgttttccc tgcaccagcc agctcaccgg cgtcgctgcc cagtaaagaa
    13441 gatccttcgc gctccagcgc cgcgcccagc tgcacgcctt ctaatgccag cgccgcctga
    13501 atcaactggc ggcccatacg ccccccggct cccgcgatgg caacgcggat gtttgcatca
    13561 tgcatagcta ttctcttttg ttaatttgca tagaccgttt tcagagtaac cagcgcatcg
    13621 ctacgccgcc agccaaaaac gctgataatt agaaattaat gataaacagg gagggatatc
    13681 agtgaaaggc atgagtcgat gaatgactgc atgccgataa catgtgacag gttacgacgc
    13741 cagagccagc acctcagcca cccactgctg gaagcctttc acatccagat ccaatgccac
    13801 ctttacattg gctggtttgc ccaggcaacc gtcgatatca accaccgtcg tgcctgaggt
    13861 aaattcgcct tgagtttcta ccgcgacaaa acacggcttg agggtgaata agtccgggcg
    13921 caccagccag gcgatggcgc agagatcgtg cattcgcaga ccgctttgca tactgccgct
    13981 acggtagtgg ctaaacagtg catgaagcat ttttccggtg cgattcaact ccggcaaagt
    14041 agcgagatag tcaggggtta atattgcctg attggtgaca tccagaccgc acatgacgat
    14101 ttcgattccg ctacggaaga cacaggcagc cgcttctgga tcggcagcga tattaaactc
    14161 ggcgtttggc gtacagttgc cgcgtccggc agaaccaccc atgatcacca gacggcgaat
    14221 atacggcttg cattccgggc attgtgaaag taacagcgca atattggtta acgggccaat
    14281 ggccaccagg gtaacaggct ctggtgcacg catcagggca tcccgaatcg ccagaaacgc
    14341 cggtatcccg agcggctttc ggttgtgctc aacaaagtcg tagccagcca ttcccgattc
    14401 gccgtgcaca gatgccgcat cacgcggtgc gcgtaccagc ggcacagcgg ccccttgggc
    14461 gagcggaatc tccgcattcc agaaatgcag cagttgcagg gcattgcggg tagttttctc
    14521 aaccgagaca ttacccgcga cggtggtcat cagttgcagg tcgagttcgg gtgcaaaaat
    14581 cgcggcggca atggcgacgg catcgtcaat gccggggtca gtatcgagga agataggtaa
    14641 acgcatgttt tctccataaa aaatgccggt aacaagaccg gcattttcgc ataacttagg
    14701 ctgctaatga cttaatcgac ttcacgaata tcgacacgca gctctttcgg cacttcgaaa
    14761 acaatgtttt cttcacgtcc ttccagcgga atggcttcac caccgcccag ctgttgcaaa
    14821 cgtgccacca cattctgcac cagaatatcc ggagccgatg cgcccgcagt cacgccgatg
    14881 catttaacct ctttcaccca ctcttcctgg atatctttcg catcatcaat caaaaacgcg
    14941 cttttgccca tacgctgggc cagctccgcc agacggttgg agttagagga gtttttcgaa
    15001 ccgaccacca gcacaacttc cgcctgttcg gccagggcac gtaccgcttc ctgtcggtta
    15061 gtcgtggcgt agcagatgtc atctttgcgc ggaccgacaa ttttcgggaa gcgtttacgc
    15121 agcgcgtcga tcacatcaga cgtgtcatcc accgacagcg tggtctgggt cataaaggag
    15181 agtttctctt cgtttttgac cgtcagtttc cacacatcgt ctggtgattc aaccagatac
    15241 attccccctt ccgggttgct gtactgaccc atcgtgcctt ccacttccgg gtgcccggcg
    15301 tgaccgatga gaatagattc ttcgccacgg cgactagcgc gggcgacttc catatgcact
    15361 ttggtcacca gcggacaggt ggcatcaaac accgtcaaat cgcggctttt tgcttcgtta
    15421 cgtaccgcct gagaaacacc gtgtgcggag aaaatcagga tcgcgccgtc cggtacttcg
    15481 ctaatctgct caataaagat agccccacgc tcacgcaagc tatcgaccac atagcggtta
    15541 tgtaccactt cgtgacggac atatatcggt gcgccgtaaa tggccagcgc gttttcaaca
    15601 atgctgatag cgcggtctac cccggcacaa aaaccacgcg ggttggccaa caggatctgc
    15661 atgttacgcc tccagtgccg gatcgatttc cagcacttca atatcaaaat gaacggtctg
    15721 cccggccagc ggatggttga aatcaacggt aatggagtcg ccgttaattt cgcggatcac
    15781 gccaggcatc tcactgccat ccattgcggt aaaaagcatg attgcgccaa tttctggctc
    15841 gcctgcatcc ataaattcac ggcgggagaa gtactgaatc aggtccggtg acggcacgcc
    15901 aaacgccgca tctggctcca acgagaaggt ggttttatcg cccactttca gccccaacag
    15961 gtgttgctcc agcccttcag aaagagaagc atcacccagg cggaacagcg ccggtttacc
    16021 gttgttgcgg gtagactcgg cggtggtgcc atcgtcgagt tttagcgtga agtgcaccag
    16081 gacggcgcta ttgctctgta cagattcaga catgcaggtt tgctcttata tttaaggatt
    16141 tttgccggat gcggcgtaaa cgccttatcc ggcctacgat ttcgcagcaa tctgtaggcc
    16201 ggataagatg cgtcagcatc gcatccggca gggtttatta ttgttttttc gctttagaag
    16261 gtaaaaaacc ttccagcaca atcagtgccg caccgacaca aatggcagta tcggcaaggt
    16321 tgaaggtggc gaagtgccag tcgccgacgt agaagtcgat catatcgaca acgaagccgt
    16381 gccacaggcg gtcgaacagg ttgcccagcg cgccgccaat aatcagcgcg taagcgatat
    16441 tgtttagctt ctgcgtggcc ttcgagcgat acatcatcac tgccaggatc acgctaatac
    16501 caatcgcaat accggcaaag aaccaacgct gccaaccgcc gctatcggca aggaaactaa
    16561 acgccgcgcc atagttacgc gcataatgca gattaagcga cgggaacagc gggaccgtat
    16621 cccccagagc aaagttctgg aggatcaggt atttgctgcc cagatcgata atcagcacga
    16681 ctaccaccag ccacagccag cgtagccctg ttgaacagat cgattgactc atcaggcaaa
    16741 cttacgtttt tcaccgtcac cggcgacgtt gctgacacag cggccgcaga tttctgcgtg
    16801 ttccgccacc ttgccgacat cctgggtgta gtgccagcag cgtgggcact tctcaccttc
    16861 ggctttactc aacgcgactt tcagcccttt gagtacttcg ctctgctgag catcagcagg
    16921 tgcgtcgtta tagtctgcaa cggtagcgcc ggaggtcaac aggacaaatc gtaattcatc
    16981 gcccagcgcg gtcagtttcg ccgacagttc cggttctgca tacaaggtta ctgccgcttc
    17041 cagcgagcca cccactttct tgtcggcacg cgcttgctca atgactttgt tcacttcgcc
    17101 acgcactttc aacagctcgt cccagaacgc atcgttcatc gcttcactgt ctgccaggcc
    17161 aaacaggcct tcgtaccact caccggtgaa gacgtatttt tcacgttcgc ccggcaggta
    17221 gccccacact tcatcagcgg tgaaggagag gattggtgcc atccagcgca ccagcgcttc
    17281 tgcgatgtga tacagcgcag tctggcagct acgacgcgcc acactgtccg ctttggcggt
    17341 gtactgacgg tctttgatga tgtcgaggta gaaggaaccc atctcaacgg agcagaagcg
    17401 catcagacgc tgtaccactt cgtggaaatc gtatgcttcg tacgccttga ggatgtcttc
    17461 ctgtgccgct ttcgcacaac ctacggccca gcgatccagt accaccatct cttccggttt
    17521 caccatatct tttgctggat caaaaccgtt caggtttgcc agcaggaagc gcgcggtgtt
    17581 acggatacga cgatagctat cggcagcacg tttcaggatc tcgtcagaaa cggccatttc
    17641 accggtgtag tcggttgatg ccacccacag acgcagaata tccgcgccca gtttgttcat
    17701 cacatcctgc ggcgaaacgg tattgccgat ggatttagac atcttgcggc cctgaccatc
    17761 cacggtaaag ccgtgggtca gtacctgacg atacggcgct ttgcctttca tcgcggtgga
    17821 gatcattagg gaagacatga accaaccacg gtgctggtca gaaccttcca gatacatgtc
    17881 cgctgcgtga ccggcaaatt ccggacgcac gtcaacaaca gaagagtggg tagatccgga
    17941 gtcaaaccat acatccaatg tgtccggcac tttcacgtac tgatcagctt catcgccgag
    18001 gatctctttc gcatcaagat cccaccacgc ctggatgccg tcaacttcaa cgcgttttgc
    18061 cacttcttcc attagttcga gagtacgcgg atgcagctct tccgtgtctt tgtgcacgaa
    18121 cagtgacatc ggtacgcccc aggtgcgctg acgggagata caccagtcag gacggttagc
    18181 aaccatcgac tcgatacgcg cctggcccca gtccgggatc cactgcacgc ctttgatctc
    18241 tttcagtgac tgcgcacgca gacctttctg atccatgctg acgaaccact gcggcgtcgc
    18301 gcggaagatg atcggcgttt tgtgacgcca gcagcacgga tagctgtgct gcattttctc
    18361 aacgtgcagc agcgcgcctt tttcctgcag cagcgcaacg acgatgtcgt tcgctttgaa
    18421 gacgttcacg ccatccagcg tcggataagt gcccggcaga taagtgccgt ccgggccaac
    18481 cgggttagcg gtttccaggc cgtatttctg accgatcaca tagtcgtccg ggccgtggcc
    18541 aggcgcggtg tgaacggcac cggtaccggc atccagggta acgtgatcgc cgaggattgc
    18601 cggaacgtcg aagcccataa acggatgggt aaagcgcagc aactcaagct ccgcaccttt
    18661 taccgtgccg agaatggtgt aatcggtcac gccgatacgc tgcattacgc tttcaaccag
    18721 atctttcgcc agaatcacgg cctgaccgtc gatctgcacc agcgcatagt cgaaatcagg
    18781 tgcaatagag attgcgcggt tcgcaggcag agtccacggc gtagtggtcc agatcaccag
    18841 cgagattggg ccgttaacgt tgcttacggc aaattttgct ttcagtgcat cctgatcgac
    18901 cgcctgaaaa gcaacgtcga tggacggaga agttttgtcg taatactcaa cttccgcttc
    18961 cgccagcgca gaacggcagt caacgcacca gtgtaccggc ttcgcgcctt tgtgcaggtg
    19021 accgttgccg atgattttgc ccagcgcgcg gatgatgttg gcttcagttt tgaagtccat
    19081 ggtcaggtac gggtgcgacc agtcgcccag cacgcccaga cggataaagt ctttgcgttg
    19141 accgtcaacc tgggtcgccg cgtattcgcg gcacttggcg cggaactcgg cggcggtgaa
    19201 tttctcaccc ggcttaccgt attcttgctc tactttcagc tcgatcggca gaccgtggca
    19261 gtcccagcca ggcacatacg gcgagtcata accggaaagc cctttggact tcacgataat
    19321 gtctttcaga atcttgttaa ccgagtgacc aatatgaatg ctgccattcg cataaggagg
    19381 gccatcatgc agaatgaagg tttttttgcc ttttttagcc gcacggatga tgccgtacag
    19441 atcatcatca gtccaacgcg ccagcattcc cggttcgcgc ttggcgagat cgccacgcat
    19501 cgggaaccct gtttccggca aattcagggt tgatttatag tcactcatca gattctcggt
    19561 tccgtgtttc ggtttgatta cataacaggc ttaagccggt tttgttagcc caaaaaattc
    19621 gcgggcggtt aattcatcac gcgcaatctg cgctttcagt tcgtccagcg acgcaaatcg
    19681 ctgctcattg cgtatttttt tacgcagcac tacttgtata tggcgaccgt aaaggtccat
    19741 tgcaacatct aacaaatgca cttccagctg ctggcgaata ccggcaaccg ttgggcgtgt
    19801 tccgatgttt gccacgccgg gtaacggctt ttcaccgagg cccagcactt ctaccgcata
    19861 aacccctttc accggggaaa cctgacggcg tagcggtaca ttcgccgtcg ggaaacctat
    19921 agtgcgccct aattcatcac cgtggactac acgcccggag atggcaaacg ggtgccccag
    19981 taaactttct gccagagcca gattgtcatc cgccagcgcc tgacgcacag cggtgctgct
    20041 aatacgcacg ccaccttcgc aaaaagtttg cgtactagtg atatcgaagc cgtattccat
    20101 gccagctttc tgtaataaca agaaatcgcc ttcacgacca gcgccaaagc ggaaatcatc
    20161 ccctacggca agaaatttta cgcgcaaatg cttcaccaga agatcgctga tgaaattttg
    20221 cgcggttaac gccgcgaaac gcctgtcgaa acgcacgcac agcacgtaat caacgccaca
    20281 ctctgcaagg taacgcagtt tttcccgcag ccgggtcagt cttgccgggg ctttatcggt
    20341 agcaaacagt tccagtggtt gaggttcaaa aagcatcacc atcaccggta agttgcgctt
    20401 gcgcccttct tcctgcaagc cctgtaacag cgcgcgatga ccgcgatgca cgccgtcgaa
    20461 attaccaata gtcagcacac acccttcttg cggggcctgg ctgagattat gtatgccgcg
    20521 tatcagcttc atgtctggct caaaacagtg aaaatcgtcc gagtatacct tgtacagcgg
    20581 tcaaggttaa ccggcgattg agtaccgaaa tcagaaagaa gtggcttttc attgccatgg
    20641 cgcaaatcac gggaagaaag tgaccgcctg ctgcaatttt tatcgcggaa aagctgtatt
    20701 cacaccccgc aagctggtag aatcctgcgc catcactacg taacgagtgc cggcacatta
    20761 acggcgctta tttgcacaaa tccattgaca aaagaaggct aaaagggcat attcctcggc
    20821 ctttgaattg tccatataga acacatttgg gagttggacc ttggctaata tcaaatcagc
    20881 taagaagcgc gccattcagt ctgaaaaggc tcgtaagcac aacgcaagcc gtcgctctat
    20941 gatgcgtact ttcatcaaga aagtatacgc agctatcgaa gctggcgaca aagctgctgc
    21001 acagaaagca tttaacgaaa tgcaaccgat cgtggaccgt caggctgcta aaggtctgat
    21061 ccacaaaaac aaagctgcac gtcataaggc taacctgact gcacagatca acaaactggc
    21121 ttaatcgcca atttgctgaa gctttgtgaa aaagcccgcg caagcgggtt tttttatgcc
    21181 cgctgctttt atgatgcgtg agagaacaca ctaccggttg ccagatagtc aaaagatgct
    21241 ggcttttgca gacatgccgt tacactttct gaactcttcg tggcttaaga atcgtagaac
    21301 catcggctga tacattctga acaagaatat ctactcaatc tccagactcc cctacaaaga
    21361 atgtggatat tgtgatacac attgaggtat taaacttaag accactctat ttttgaat
//
LOCUS       DAJOWB010000080        20807 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJOWB010000080 DAJOWB010000000
VERSION     DAJOWB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20807
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.080"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(121..1770)
                     /gene="actP"
                     /locus_tag="OHO85_003170"
     CDS             complement(121..1770)
                     /gene="actP"
                     /locus_tag="OHO85_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCQ2672513.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1767..2081)
                     /locus_tag="OHO85_003171"
     CDS             complement(1767..2081)
                     /locus_tag="OHO85_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCQ2672514.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2282..4240)
                     /gene="acs"
                     /locus_tag="OHO85_003172"
     CDS             complement(2282..4240)
                     /gene="acs"
                     /locus_tag="OHO85_003172"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCQ2672515.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4632..6068
                     /gene="nrfA"
                     /locus_tag="OHO85_003173"
     CDS             4632..6068
                     /gene="nrfA"
                     /locus_tag="OHO85_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCQ2672516.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPPKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6113..6679
                     /gene="nrfB"
                     /locus_tag="OHO85_003174"
     CDS             6113..6679
                     /gene="nrfB"
                     /locus_tag="OHO85_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCQ2672517.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6676..7347
                     /gene="nrfC"
                     /locus_tag="OHO85_003175"
     CDS             6676..7347
                     /gene="nrfC"
                     /locus_tag="OHO85_003175"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCQ2672518.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7344..8300
                     /gene="nrfD"
                     /locus_tag="OHO85_003176"
     CDS             7344..8300
                     /gene="nrfD"
                     /locus_tag="OHO85_003176"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCQ2672519.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPTVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8320..10038
                     /locus_tag="OHO85_003177"
     CDS             8320..10038
                     /locus_tag="OHO85_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCQ2672520.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10031..10414
                     /gene="nrfF"
                     /locus_tag="OHO85_003178"
     CDS             10031..10414
                     /gene="nrfF"
                     /locus_tag="OHO85_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCQ2672521.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10411..11007
                     /gene="nrfG"
                     /locus_tag="OHO85_003179"
     CDS             10411..11007
                     /gene="nrfG"
                     /locus_tag="OHO85_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCQ2672522.1"
                     /translation="MKQPKVPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11349..12662
                     /gene="gltP"
                     /locus_tag="OHO85_003180"
     CDS             11349..12662
                     /gene="gltP"
                     /locus_tag="OHO85_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCQ2672523.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12740..13429)
                     /locus_tag="OHO85_003181"
     CDS             complement(12740..13429)
                     /locus_tag="OHO85_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCQ2672524.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13523..15670)
                     /gene="fdhF"
                     /locus_tag="OHO85_003182"
     CDS             complement(13523..15670)
                     /gene="fdhF"
                     /locus_tag="OHO85_003182"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15251..15253),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCQ2672525.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15868..17334)
                     /gene="mdtP"
                     /locus_tag="OHO85_003183"
     CDS             complement(15868..17334)
                     /gene="mdtP"
                     /locus_tag="OHO85_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCQ2672526.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17331..19382)
                     /gene="mdtO"
                     /locus_tag="OHO85_003184"
     CDS             complement(17331..19382)
                     /gene="mdtO"
                     /locus_tag="OHO85_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCQ2672527.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRAMGIIIGTVVSTVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19382..20413)
                     /gene="mdtN"
                     /locus_tag="OHO85_003185"
     CDS             complement(19382..20413)
                     /gene="mdtN"
                     /locus_tag="OHO85_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCQ2672528.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20432..20707)
                     /locus_tag="OHO85_003186"
     CDS             complement(20432..20707)
                     /locus_tag="OHO85_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672529.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4691 a         5510 c         5702 g         4904 t
ORIGIN      
        1 tacgactgtc aaaaatcgct cattttttaa tgagtttatt tgtttaatat tatgggaaaa
       61 ggtgatgcat gctggagagg aagagtattc cccggtcagg cgaccgggga aggggagaga
      121 ttaatgtgcg cggccttgct caacgccaaa gccggtctgg gagcggataa actgtgcacg
      181 gaacagttca cgctcgcgcg caccttccgc tgagttatcg gttgccgaga agaaccagat
      241 gccgaggaat gccacgctga tagagaacag cgccgggtat tcatacggga agatggcttt
      301 ttcgtgacca aggatctgta cccaaatcgt cgggccgagg atcatcagta ccactgcggt
      361 aatcagcccc agccagcccc ccatcatcgc gccacgagtg gtcagtttcg accagtacat
      421 agaaagcaga atgatcggga agttacagct cgctgcgatg gcaaacgcca ggcctaccat
      481 gaaggcgatg ttctgattct caaacagcac gccgaggata atggcaatca cacctaaaat
      541 cagcacggtg attttcgata cccgcagttc ttcacgttcg gtcgcgcctt ttttgaagac
      601 gttagcgtac aggtcatgcg aaaccgctga tgcgcccgcc agcgtcagac cagcaaccac
      661 cgcaagaatg gtggcaaaag caaccgctga aataaaaccg aggaacaggt tgccgcccac
      721 tgcattcgcc aggtgaacgg ccgccatgtt gttaccacca atcagatggc ccgccgcgtc
      781 tttatattcc ggattcgcac caaccagcat gatcgcgccg aagccgataa taaaggtcag
      841 aatatagaag tagcccataa atccggtagc gtagaacacg ctcttacgcg cttcgcgggc
      901 atcgctgacc gtaaagaagc gcatcagaat gtgcggcaag ccagccgtac caaacatcag
      961 tcccagaccc agagagagcg cggagatcgg atctttcacc agtccgcccg gtttcatgat
     1021 gtcgacacct ttcgggtgta ccgccatcgc ttcgctgaac agattgttga agctaaagcc
     1081 gacgtgtttc atcaccataa aggccataaa gctggcaccg aacagcaaca gcacggcttt
     1141 gataatttgt acccaggtgg tcgccagcat gccgccgaac aggacgtaca tcatcatcag
     1201 cacgccgacc agcaccaccg caatgtgata gttaaggcca aacagcagct cgatcagttt
     1261 acctgcaccc accatctggg cgataaggta aagcgccacc accaccagag aaccacaggc
     1321 cgaaagaata cgaatcggcc cttgtttcag acggtaagag gccacatcgg caaaggtgta
     1381 gcgccccagg ttacgcagac gttcggcaat caggaacaga atgatcggcc agcccaccag
     1441 gaagcccagc gagtaaatta agccgtcata gccggaggta aacaccagcg cggagatccc
     1501 caaaaatgag gcggcggaca tatagtcacc ggcaatcgcc agcccgttct ggaagccagt
     1561 gatattgccg cctgcggtgt agtagtcgct acgagaacgt acgcgttttg atgcccagta
     1621 ggtaatgccg agcgtaaaca cgacgaaaat caggaacata ataatcgcct gccagttcgt
     1681 tggctggcgc tctacggccc cgctaatagc atccgcggcg ttagctgcga aagggagtgt
     1741 ggcggcaagc gccgtcagaa ctcttttcat gatgcttgta cctcatgcag gacttcgtta
     1801 ttaagacggt cgaattcgcc gttcgcccgc cagatgtaga taccggtgag aacaaaggag
     1861 atcacaatca ctccaacacc aatcggaata ccgcgtgtga cgctggtgtt cggattcagc
     1921 ggcgtgccca gccagccggg cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc
     1981 agcataataa tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa
     2041 tgcgcattgt cttctatccg ctgataaata gtgccattca tcacagattc tccagaggta
     2101 atgtagggat tgttttaatt cccgctccct tatgggagaa ggttaacgct cgggtaaccc
     2161 ttgccgaatg taggccggat aaggcgttat atgccgcatc cggcaatcaa tgcctgatgc
     2221 gacgctgtcg cgtcttatca ggcctacaaa ccgttaccga ttcgcatccg gcaattgtgg
     2281 gttacgatgg catcgcgata gcctgcttct cttcaagcag cttctcgaca acgccaggat
     2341 cggcaagcgt cgaggtatcg cccaggttgc tggtatcgcc cgccgcaatt ttgcgcagaa
     2401 tacggcgcat aattttgccg gaacgggttt taggcaggga gtcggtccag tgcagcacgt
     2461 ctggcgtcgc cagcgggcca atctctttac gcacccagtt gcggacttct gcgtacagtt
     2521 ctggtgacgg ttcctccccg tgattaagcg tgacgtaggc gtagatcgcc tgacctttaa
     2581 tattgtgcgg aatacctact acggcggctt cggcaatctt cggatgcgcc accagcgccg
     2641 actcaatctc tgccgtcccc agacggtgac cggagacgtt cagcacgtcg tccacacgcc
     2701 cggtgatcca gtaatagcca tcttcatcgc gacgcgcgcc gtcgccgctg aaatacatat
     2761 ttttgaaggt ggagaagtag gtctgttcaa aacgttcgtg atcgccaaac agcgtacgcg
     2821 cctgacccgg ccaggagtcg gtgatcacca ggctgccttc ggtagcgcct tccagcgggt
     2881 taccttcgtt atcgaccagc gccggttgca cgccgaagaa cggacgtgtt gccgaaccgg
     2941 ctttcagctc ggtagcgcca ggcagcggcg tgatcatgaa accgccagtt tcggtctgcc
     3001 accaggtatc gaccaccgga catttctcgt tgccgatttt tttccagtac cactcccacg
     3061 cttctgggtt aattggctcg cccacggaac cgagaatgcg cagcgacgaa cggtcggtac
     3121 cctcgatcgc tttatcgcct tccgccatca gcgcgcggat cgccgtgggt gcggtataga
     3181 gaatattgac ctgatgcttg tccaccacct gcgccatacg ggcaggcgtc ggccagttgg
     3241 gtacgccttc aaacatcagc gtggtcgcac cgcaggccag cgggccgtac agcaagtaac
     3301 tgtgtccggt cacccagccc acatcggcgg tgcaccagta gatatcaccc ggatgataat
     3361 caaagacata tttaaaggtc agcgccgcgt acaccagata accgccggta gtatgcagca
     3421 caccttttgg cttaccggta gaaccggagg tgtagagaat aaacagcgga tcttcggcgt
     3481 tcatctcttc cgcctggtgc tgatcgctcg cttgctcaac caggtcgtgc caccacaggt
     3541 cgcgcccttc ctgccagtca attttcccgc cagtacgctt cagtaccacc acatgctcta
     3601 cgctggtgac gttcgggttt ttcagcgcgt catcaacgtt tttcttcagc ggaatactgc
     3661 gcccggcacg cacaccttcg tcggaagtga tcaccagtcg tgagttggaa tcaataatgc
     3721 gcccggcaac ggcttccggc gagaagccgc cgaaaatcac cgaatgcacc gcgccaatgc
     3781 gggcgcaggc cagcatcgca accgcggctt ccggcaccat cggcatataa atcgccacca
     3841 catcaccttt tttaatgccc agctcgagca gggtattggc gaagcggcag acgtcgcggt
     3901 gcagctcttt atagctgata tgtttgctct ggctggcgtc gtcgccttcc cagatgatgg
     3961 cggtgcgatc gccgttttct tgcagatggc ggtcaaggca gtttgccgcc agattcagcg
     4021 tgccgtcctc gtaccattta atggacacat taccgggggc aaaggaggta tttttcacct
     4081 tctggtacgg tttgatccag tcgagaattt ttccctgttc gccccagaag gtatcaggta
     4141 cgttaataga ttgttgatac atcgcctcgt actgctgagg gtttatcagg caacggtctg
     4201 cgatgttggc aggaatggtg tgtttgtgaa tttggctcat gcttttgttc tccttgtagg
     4261 atgttaataa tatgtggcat aagcgttaaa tgtaggggtg ttggcagttt tgtttagtat
     4321 ttgggcgaca gatcacgcaa aagtagaatt gtgcaaataa acggcagggt aatttttgaa
     4381 ggtcaccaac aaaagttgat taattctttg aggaacatgc agttatgcat gctgttgaaa
     4441 aagaggaaga tactgactaa ctctaaagtg gtattttaca tgcacttaca attgattaaa
     4501 gacaacattc acagtgtggt tatttgttac acataggggc gagcaatgtc atgacagtgt
     4561 aggtgcggtt actgtcgtga aaacaataaa aaccgccatt gcaacaatgg cgcaattcgg
     4621 atgaagcccc tatgacaagg ataaaaataa acgcacgccg tatcttcagc ttattgattc
     4681 cttttttctt tttcacttct gttcacgctg aacaaacggc agctccccca aaacctgtaa
     4741 ctgtggaagc gaagaatgaa acctttgccc cgcagcatcc cgatcaatat ctctcctgga
     4801 aagccacctc ggaacagtca gagcgtgttg acgccctggc ggaagatcca cggctggtga
     4861 tcctgtgggc ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga catgcctttg
     4921 ctgtgaccga tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct gaagatggtc
     4981 cgctaccgat ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg atccagaaag
     5041 acggcgaaga tggctacttc cacggtaagt gggcgcgcgg cggcccggaa atcgtcaaca
     5101 acttaggttg tgccgactgc cataacaccg cctcaccaga gttcgccaaa ggcaaaccgg
     5161 agttaaccct ttcccgtccg tatgcggctc gcgcgatgga agccattggt aaaccttttg
     5221 agaaagccgg acgtttcgac cagcaatcga tggtttgcgg tcagtgccat gtggagtatt
     5281 acttcgacgg caaaaacaaa gcggttaaat tcccgtggga tgacggcatg aaagtcgaaa
     5341 atatggagca gtattacgac aaaattgcct tctctgactg gactaactcc ctgtcgaaaa
     5401 cgccaatgct gaaagcgcag cacccggaat atgaaacctg gacagcgggc attcacggta
     5461 aaaacaacgt gacctgtatc gactgccata tgccaaaagt gcagaacgcc gaaggcaaac
     5521 tctacaccga ccataaaatt ggtaatccgt ttgataactt cgcccagact tgtgcgaact
     5581 gccataccca ggacaaagct gccttgcaaa aagtggtcgc ggaacgtaag cagtcgatta
     5641 acgacctgaa aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa gcgaaagcag
     5701 cgctggatgc aggcgcgacg gaagccgaaa tgaagccaat tcaggacgat atccgtcatg
     5761 cccagtggcg ctgggatctg gcgatcgctt cccacggcat tcatatgcac gcaccggaag
     5821 aaggtttacg gatgctcggt acggcgatgg ataaagcggc ggatgcacgc accaaactgg
     5881 cgcgcctgct ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat atctcaacca
     5941 aagagaaagc ccagcaggcc attggcctga acatggaaca aatcaaggcc gagaagcagg
     6001 acttcatcaa aacggtgatc ccgcagtggg aagaacaggc acgtaaaaac ggtctgttaa
     6061 gccaataacc ccgttccgcc tcgcaagggg cggaaaacac aatggagtga atatgagcgt
     6121 attacgttcg ttattaactg ccggggtgct ggcgtcgggc ctgttgtgga gcctgaacgg
     6181 aattaccgct acccctgcgg cgcaggcatc tgatgatcgc tacgaagtta cccagcagcg
     6241 taacccggat gccgcctgtc tggactgtca taagccagat accgaaggta tgcatggaaa
     6301 acatgcttcc gttatcaacc cgaataacaa actgccggtc acctgcacca actgtcacgg
     6361 ccagccatca ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta acgagccgat
     6421 gtacaaggtt ggagagcaga acagcgtctg tatgtcctgt catctgccgg aacagttgca
     6481 aaaagcgttc tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca gctgccattc
     6541 cctgcatcca cagcaagata cgatgcagac gttaagtgac aaaggacgga ttaagatttg
     6601 cgtcgattgc cacagcgatc agcgcaccaa tccgaacttt aacccggcgt ctgttccgtt
     6661 gcttaaggag cagccatgac ctggtctcgt cgccagtttc tcaccggcgt cggcgtgctg
     6721 gcagccgtca gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat caatggggtg
     6781 cgttacggca tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg tatggatgct
     6841 tgtcgggaag tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat tcgtagcgag
     6901 ccgcagggcg aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg ccagcactgc
     6961 gatcatgcgc cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga tgccgctagc
     7021 gggattgtcg atgttaatcc tgatctctgc gtcggttgtc agtactgcat cgccgcctgt
     7081 ccgtaccgcg tgcgctttat ccatccggtc acgaagacgg cggacaaatg cgatttctgc
     7141 cgtaaaacca atttgcaggc cggtaagctg cccgcgtgcg ttgaggcttg cccgacgaag
     7201 gcgctgacgt ttggcaatct ggacgatcct aacagtgaga tttcgcaact gctgcgccag
     7261 aagcctactt accgctacaa gctggcgctg ggaaccaaac cgaagctgta ccgcgtaccg
     7321 tttaaatacg gggaggtgag ccaatgacgc agacttccgc atttcatttt gaatcgctgg
     7381 tgtgggactg gccgattgcc atctacctgt ttttgattgg tatttctgcc ggtctggtga
     7441 cgctggccgt gctgttacgt cgcttctacc cgcaggcggg cggtgcagac agtacgttgc
     7501 tgcgcaccac gctgattgtc gggccgggcg cggtgatcct cggtctgttg atcctcgtct
     7561 tccacctgac aagaccgtgg accttctgga agctgatgtt ccactacagt tttacctcgg
     7621 tgatgtcgat gggggtgatg ctgtttcagc tctacatggt ggtgctggtg ctgtggctgg
     7681 cgaaaatctt tgaacatgat ttgcttgccc tgcaacaacg ctggttgccg aagctgggga
     7741 tcgtgcaaaa ggttctgagc ctgctgacgc ccgttcatcg cggactggaa acattgatgc
     7801 tggtgttggc ggtgttgttg ggggcttata ccggctttct gctgtcggcg ctgaaatcgt
     7861 atccgttcct caataacccg atcctgccgg tgctgttcct cttctccggc atctcgtccg
     7921 gtgcggcggt ggcgctgatc gccatggcga tacgccaacg cagtaacccg cattccacgg
     7981 aagcgcagtt tgtacaccgt atggaaatcc ccgtggtatg gggtgaaatc ttcctgctgg
     8041 tggcgttttt tgtcggtctg gcgctgggcg atgacggtaa agtgcgtgcg ctggtggcgg
     8101 cattaggtgg cggtttctgg acgtggtggt tctggcttgg tgtcgccggg ctggggctga
     8161 ttgtgccaat gttgctcaaa ccgtgggtca atcgcagttc cggcattcct accgtgttgg
     8221 cggcgtgtgg ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt ctctacgccg
     8281 ggcagttaac ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt ccttcctgaa
     8341 gtcggttttc tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc gttgacggcc
     8401 tttgcgggag tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat cggcattctg
     8461 gcgcagttcg cgctcctgct gctcgccttt ggcgtactga cgtattgttt tctcatcagc
     8521 gatttctcgg tcatttatgt cgcccaacat agctacagcc tgctgtcgtg ggaactcaaa
     8581 ctggcggcgg tgtggggcgg ccatgaaggt tcgctgctgc tttgggtgct gctgctttcc
     8641 gcctggagcg cgctgtttgc ctggcattat cggcagcaaa ccgatccgct atttccgctg
     8701 acgctagccg ttttatctct catgctcgcc gcactgctac tgtttgtagt gctgtggtcc
     8761 gatcccttcg tgcggatatt tccaccagca atcgaaggcc gcgatctcaa tccgatgctg
     8821 caacatcccg gtcttatctt tcatccaccg ctgctttacc ttggctatgg cggtttgatg
     8881 gtggcggcga gcgtggcgct ggcgagttta ctgcgcggcg agtttgatgg tgcctgcgcc
     8941 agaatttgtt ggcgctgggc actacctggc tggagcgcat taacggcggg gatcatcctc
     9001 ggttcctggt gggcctattg cgaactgggc tggggcggct ggtggttctg ggatccggtg
     9061 gaaaacgcct ctttattacc ctggctttct gccactgcgc tgctgcacag tttgtccctg
     9121 acacgccagc gggagatttt ccgccactgg tcgctgttgc tggcgatagt aactctgatg
     9181 ctgtcgctac tgggtacctt aattgtccgt tctggcattc tggtttcggt tcatgcgttc
     9241 gcactggata acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat tagccttgcg
     9301 tctctggctc tgtatggctg gcgagcgcgg gacggtggtg cggtggtgcg tttttcgggg
     9361 ttatcgcggg aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt gctactgatc
     9421 gtgctggtgg gaacgcttta tccgatgatt tacggtctgc tgggctgggg acgcctctcc
     9481 gttggcgcgc cgtattttaa ccgcgcgacg ttaccgtttg gcctgttgat gctggtggtg
     9541 attgtgctgg cgacgtttgt ctctggcaaa cgcgtgcagc ttccggcgct ggtagctcat
     9601 gcgggcgtgc tgttatttgc cgcggggatc gtggtttcca gcgtcagccg tcaggagatc
     9661 agcctgaatt tacagccggg tcagcaggtg acgctggcag gatacacctt ccgttttgag
     9721 cgcctcgatc tgcaagccaa aggcaattac accagcgaaa aagcgatagt ggcactgttt
     9781 gaccatcagc aacgcattgg tgaactgatg ccggagcggc gtttttacga agcacgtcgt
     9841 cagcaaatga tggaaccgtc aattcgctgg aacggcatcc atgactggta tgcggtcatg
     9901 ggtgaaaaaa ccggagcgga tcgttacgct tttcgtttgt atgtacaaag cggtgtgcgc
     9961 tggatctggg ggggaggatt gttgatgatt gcgggcgcat tgttaagcgg atggcggggg
    10021 aagaagcgcg atgaataaag ggcttctcac gctactgctg ttatttacct gttttgcgcg
    10081 cgctcaggtc gtagacacct ggcaattcgc caatccacaa caacagcaac aggcgttaaa
    10141 tattgccagc cagttacgtt gtccgcagtg ccagaatcaa aacttactgg aatccaacgc
    10201 gccggtggca gtcagtatgc gccatcaggt ttacagcatg gtggcggagg ggaagaacga
    10261 agtcgaaatc attggctgga tgaccgaacg ctacggagat tttgttcgct ataacccacc
    10321 gttaacgggt cagacgctgg tgttatgggc gctgccagtg gtgttgttac tgctgatggc
    10381 actgatcctc tggcgagtga gggcgaagcg atgaaacagc ccaaagtacc ggtgaaaatg
    10441 ctgacaacac tcacgatatt gatggtattt ctctgtgtcg gcagttatct gttaagccca
    10501 aaatggcagg cggtacgtgc ggaatatcag cgtcagcgcg atccgctgca tcagtttgcc
    10561 agccagcaaa ccccggaagc gcagcttcag gcattgcagg ataaaatccg cgctaatcca
    10621 caaaacagcg aacagtgggc gttactgggc gagtactatc tgtggcaaaa cgattacagc
    10681 aattcgctgc tggcgtaccg tcaggcgttg caactacgtg gtgagaacgc tgaactgtat
    10741 gcggcgctgg cgacggtgct ttattaccag gccagccagc atatgaccgc ccagactcgc
    10801 gcaatgatcg ataaagccct cgcgctggac tctaacgaaa tcaccgctct gatgctgctg
    10861 gcttccgatg cgtttatgca ggcgaattac gcgcaagcca tcgaactatg gcaaaaagtg
    10921 atggatctca actcaccccg gatcaaccgg acacagctgg tcgagtcgat taatatggcg
    10981 aaattgttgc agcggagatc agattaatca tctggtgatg aagattatgt tattgagttg
    11041 tataaaaaat aatcgaatga acatatgcca aaaataatca ccaatcagta ttattgcaga
    11101 ttaaacaaat aaaaatcttt ccataacaaa tggttattca ttaatcctgc cataaactcg
    11161 cgttattatg cattaatgca gcgaaaagct ctgttgttaa agggttgcgc aacataccgc
    11221 gcaaatgata ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac acaattcatg
    11281 ccctggcagt atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc attgaggaag
    11341 ttattctgat gaaaagtata aaattcagcc tggcctggca gattctgttt gctatggtgc
    11401 tgggcattct cctgggaagc tacctgcact accatagcga cagccgcgac tggctggtcg
    11461 tcaatttgct ctctccggcg ggtgatatct tcatccatct gatcaaaatg attgttgtgc
    11521 cgattgtgat ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc aaacagctcg
    11581 ggcgtattgg cgcgaaaacc attatttact tcgaggtgat caccaccgtc gccatcattt
    11641 tggggatcac tctggcgaac gtcttccagc ccggtgccgg ggtggatatg tcgcagctgg
    11701 cgaccgtcga tatctcgaaa tatcagagca ctacggaagc ggtacaaagc agttcccacg
    11761 gcattatggg cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg atggcgaaag
    11821 gcgaaatgct gccgatcatc ttcttctcgg tgctgtttgg tctggggctt tcctccctgc
    11881 ccgcgacgca tcgtgaaccg ctggtgaccg tgttccgctc catctctgaa accatgttta
    11941 aagtgactca catggtgatg cgttatgcac cggtgggtgt gtttgcgctg attgcggtga
    12001 cggtggctaa ctttggtttc tcttctctgt ggccactggc gaaactggtg ctgctggtgc
    12061 atttcgccat tctgttcttc gcgctggtag tgctgggaat tgtggcgcgc ctgtgcgggt
    12121 taagcgtctg gatcctgatt cgtattctga aagatgagct gattctggcg tactccactg
    12181 ccagctctga aagcgtactg ccgcgaatta ttgagaagat ggaagcctac ggagcaccgg
    12241 cgtcgatcac cagcttcgtg gtgccgaccg gttactcttt taaccttgat ggttcgacgc
    12301 tgtatcaaag tattgccgct attttcatcg ctcagctgta tggcattgac ctgtccatct
    12361 ggcaggaaat cattctggtg ctgacgctga tggtgacctc gaaagggatt gctggcgtgc
    12421 cgggcgtgtc gtttgtggtg ctgctggcaa cgctgggtag cgtaggtatt ccgctggaag
    12481 gcctggcgtt tattgctggt gttgaccgta tcctcgacat ggcgcgtact gcgctgaacg
    12541 tggtgggtaa tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa tttgaccgca
    12601 aaaaggcgct ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact gcggatcaat
    12661 aattgaagat tgccggggtt atccacccgg caatgtgtgg atgcctgatg cgacgctggt
    12721 gcgtcttatc aggcctacct taaccgttgg ttaatttttc aaactcttca cacccggtat
    12781 caaacccttc catacagctc aggttcaacc agtgcagcgc cttttgcttg ttcttctcga
    12841 taaaaccttc ttcaccgttt aagaacatca ttcccgccca gtactcggaa taaccggttc
    12901 gggaaattgc agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg gccttaatgc
    12961 ccacgccgtt ggcgtaaatc aaaccaagca gcatttgggc atcgacggca gagtcgttct
    13021 ccagatcttc cgaggcgttt tctaacagcg aaatcgcctt tggataatcc ggtttacccg
    13081 cttgagtatt taccagaata tgcgccagcg ttacttcacc ttctttactg cccgcttgcg
    13141 ccgctttctc tgcaagaact tttgcttgtg gatagtccag actgaccgga ttggtgattt
    13201 tgatctgcgc cagcagcgcg caggcatcag catcaccgct gtcggcggct ttctgtgccc
    13261 aatattcggc tttgctcaaa tcgccggagc taaaccagct gtcggcaaga aaatattgtg
    13321 cgcgtcggtc cccggcctct gctgccttta aatactggct gccaggctcg gagtcgttcg
    13381 cgtgggcaaa gaatgtcaaa aacaacatca atgcgataat ttttttcatt ttgataatac
    13441 gattgggtag ggagtaacca gtataaagag ttcgaatgga taaaaaaaca gcctccgaaa
    13501 ggaggctgta gaaaggactg tattacgcca gtgccgcttc gcgcaggcga gttttcaact
    13561 tgttgtactc gtcaatcacg tactgctcgg cggcgcgctg atcggcgatc ggctcgacgc
    13621 gcacggcgca gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt tcggtaacca
    13681 gctcgttaca ggcaccaatc caccactggt aggtcatgta aatcgcccct ttgttcggac
    13741 gatcgctgac ctgcgcacgg gtgataattt tgcctttacg cgagtgcacc caaaccaatg
    13801 cctcatcttc aatacccaga cgtttggcgt cttcggtatt gatttgtgcg tagccaggtt
    13861 catcagccag cgccgccagt gccgcacagt tgccggtcat cgaacggcaa gagtagtgac
    13921 caacttcacg caccgttgac agtaccatcg ggtactcgtc ggtgagtttg tcgattggcg
    13981 ctacccagtc gcaggtgaag aactgtgcca gaccgttcgg ggtatcaaac ttctctttaa
    14041 acagataaga agtcccctga tcggcatctg aagtatcgcg gcaaggccac tgaatgaagc
    14101 ccagttcgcc cattttctcg taagtcgcac cgtagaaatc cgggcacaga tgacgcaact
    14161 catcccagat ctcctgggtg ttgttgtagt gcatcggata acccatacgg gtggcgattt
    14221 cactgatgat ttgccagtcc gttttcagat cccatttcgg ttcaactgct ttgaagaaac
    14281 gctggaagcc acggtcagcc gcagtaaaca cgccttcatg ctcgccccac gacgttgacg
    14341 gcaaaatgac atccgccgcc gacgcggttt tggtcataaa gatgtcctga acgataacca
    14401 gttccagatc ttcaaaggct ttacgtactg ccgacagctc cgcgtcagtt tgcagcggat
    14461 cttcgcccat aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc ggcagctcgc
    14521 tgatgcgata accggtatga gcaggcaggc tttccacgcc ccaggctttg gcgaatttct
    14581 cgcggttagc cggatctttc acgtactggt atcccggata cgtatccggc agcgcgccca
    14641 tatcgcaggc accctgcacg ttgttctgac cacgaaccgg gtttacaccc gcatgcggct
    14701 taccgaggtt accggtcagc atcgcgaggc tggtcagaga acgcacggtt tccacgccct
    14761 ggtagaactg ggttacaccc atgccccaca ggatggcggc gcttttcgcc tgggcataca
    14821 tccgtgccgc ctgacgaatc tcactggcgc tgacgccggt gatatcttca accgactccg
    14881 gcgtgtagcc ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg gaagcgacga
    14941 acgctttgtc gtacagattt tcttcaataa tgacatggcc catcgcattc aacagcgcga
    15001 tgttcgagcc gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt tcaattttgc
    15061 gcggatcgca gacgataatt ttcgccccgt tacgtttagc gttaattacg tgattcgcca
    15121 cgattgggtg ggaatccgcc gggttgtacc cgaaaacgaa cactaaatcg gtattatcaa
    15181 tttcgttaat agcattgctc attgcgccat taccgaccga ttggtgcaga cctgcaaccg
    15241 atgggccgtg tcagacacga gcgcagcagt caacgttatt ggtaccaata acggcgcgcg
    15301 caaatttttg cattacatag ttggtttcgt tacccgtacc acgcgaggag ccggtcgtct
    15361 ggatggcatc cggaccgtac ttctctttga tggcgctcag gcgctcggca acgtaattca
    15421 gtgcctcatc ccaggaaaca ggttcgagtt tgccgccacg ctggcgacgg atcatggggg
    15481 ttttcaggcg cggggtcagg atctgggtat cgttaatgaa atcccagcca taataaccct
    15541 tcagacacag ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg acgattttgc
    15601 cgttatcgac gaccaggttg attttgcaac ctgatgcgca ataggggcaa accgtgacga
    15661 cttttttcat cggtctcgct ccagttaatc aaatcacgca tacgcgctct cgactacagt
    15721 atgcatcttt tatgccacat tttatttggg gtcattccct gatattacgg gcactattta
    15781 ttcaaaactc tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa cgaaataccg
    15841 cgtgacagcc atcacgcggc agacatttta ttttttctcg acgacgggag ccgcctgata
    15901 cccgccgccc agcgatttca tcaactgaat gctttggatc acccggcggc tgtccagcat
    15961 cagtaatgac atctcttcgg caagtactgg caaccgggct tcagtggcct gtaagcggct
    16021 ggttaagccg cgctgatagg cggcctcggc agcgcgctgg gtgaagcgcg ttgcttccac
    16081 gcgttcagcc tgcatttctc gctcgtcgtt gagcgtttgc agacgcgtgc cgttgacggc
    16141 aacgtcacgc accgcgttca gtactgactg gttgtaacgt tcaatcatca tgttgctggc
    16201 ggcgcgcgtg ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg gcaatttcag
    16261 acccgggatg aagttgaact ggcgactggt ttttttgaat aaggtatcca ggtggatggc
    16321 atcaaggccg aaaaacgctt tgatatcaaa gctcgggtag aacagcgccc gcgcggaatc
    16381 cacctgatct aatgacgcct gaacatacca gcgcatggct tgcagatccg ggcgtctggc
    16441 gagcaactca taagagagtg tcgccggaat gccggtctgg actcgtggta atgccaccgg
    16501 tttgatctcc ggcatatcgc tggcccccgc gccaatcaat gcacgcagag attctcgcgt
    16561 ttcggtgatt tgccctttga cggcagcaat ttgtttatcg accgccagaa tctgtgcccg
    16621 cgcgccgtgg aaaggcactt gtgcttccag accgtgcgcc actttactct ggtgcgcttt
    16681 caccgcgtaa tcaatcacat cgcgagtttg ttctaacaga tcgagcatct gatagctggc
    16741 ctgcatactg taataaagct gcgctacgcc cgtggtcagc gatagctcta ctgctgcggt
    16801 ttctgccagc gcggcattat gcgcgccaat ggcggcggca accgctgagc gatgcacacc
    16861 ccacaaatca agatccagtc cggcaaacaa acctactgtg gcttccgtat agtacggccc
    16921 gtccataccc agtgcgggcg catccatcgc ataagggctt aaaaagccgt tcgccgagac
    16981 gcgttggcgg ttgagcatcc ctaacgctgc gacctgtaat tgtgaaccgg catctaacaa
    17041 atcggcctgg gactgcgctt tttcttcccg cagtttcgct tcggcgaggg tgtgtgaacc
    17101 acttagcgtc cgttggatca gcgcatccag ctgcgggtca ttgagttgtt tccaccactg
    17161 cgcctgcggc cagccggagc tggcaagatg aatatcgtcg gccagtttga tttgttccgg
    17221 tttgagctgt tgatgaggtg cagaatcctt acgtaccagg gcacagccgg aaatcagcgt
    17281 cgtgctgccg agaatgctgc acaacagcag acgtgaaagt tgacgattga tcattgcgtg
    17341 gctccttgtg cctgttccgt ggcgggcgtt aatgccgggg ctgtccagtc tggcaagcgg
    17401 gcgaaaagct ggcagacgtg ttgctcctgt tttaataagg tgggcaggat ggcctgcgag
    17461 gcgggtgtct cttcaagcgt tatttgagga gaacggctga gtgcggttgc cagaccggca
    17521 gcgtatttct ccagggcgtc ggcaaactga gctgctctgt ccggctgtag tgcgttatcc
    17581 agcgcctgcg ccgagttcca ggtggcatcc caggcgtgaa gaatatcgcg gccctgacga
    17641 ataaccgttt gcgaacgttc aatcagtaag gcgcgttctt cgctgtccag ttgacgctcc
    17701 agcgccacgc gttggcacat ctcttcacag gcattaaacg ccgcatgcag accgatacga
    17761 atttgcagat aagtgcgcag agccgtgact tcctgctggc gtgggatccg cattacttta
    17821 cttaacatac ccagcgtgcc agccagtttt tgcggcagtg tgcgcgcttc actttcaggc
    17881 cagacaaagg tgtaaatcac cgtggacacc acggtaccaa tgatgatacc catggcgcga
    17941 tcgcgaattt ccaccaggtc gtacaccggg ccaaaaacgt tttcgagcgt ggcgagcgcg
    18001 aaggtgacca ccatctgtgt gccgatataa gaagagcgtt cagagctggt ggcaatccat
    18061 gcgcccaaca ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt gtccagccag
    18121 ggcatgacca gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc cccgccaaaa
    18181 cgcagcacca tcttctggta cgacgaaccg acatttgggt tagcgacgat cacgcatgtc
    18241 agcatacagg tgtgaatgcc ttcccagtcc acgccgctgt agaaggtgta acagatcaaa
    18301 caggcgagca gtgtttttac cgcgtagcgc atatagtctg gattggtaaa agcatcggcg
    18361 accattgatg gcggtttggc ggcgggcgtt ggcggcgtat tcgggtccat ctgacccagt
    18421 tgtaacaacg tctggcagat attctccagg ttacattccc gtgccgccat cgcttcactt
    18481 tcagtgatcc gccagtcgct ttgccagcac tgaccttcag caacggcatg ctgcagctta
    18541 ttgatttctg aggctaattt ttgccgaaat tcaataattg cctgagaatc agcaaaagag
    18601 gtgggatcgt agcgattcag cgtcgagtaa atgtaggtta ccgttgccac gcagctttgc
    18661 caccatgcgc tttgagttcg ccagttggca tcgtccgcga gacaaaagac attcagtttt
    18721 tgcagcgcca gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag gctgtccgtc
    18781 aggtgactaa tggcatcatc aagccgatca ttaagcgctt gatgcatttg cgtaatggca
    18841 cgactgggaa accacagcac gccgattaac gtcatcagca aggttggata gaggccaaca
    18901 acgatacacc acagcgttaa gcgcacgacc acttccggat agtcgagcat ggcggggaag
    18961 gtttgcccgt aaatggcgac aatggcgaca gcgaaaaaga ccagtcccag ccgatgggtg
    19021 cgcatcaaaa acatacagcc catcaggatc ggcccggcga tgatcaatcg gatcaacggt
    19081 tcgccgtatg accatttgta gatcaaaaac aggctgccga tctccagcac cgtggcaacc
    19141 acaaacaaga tcgcgacaaa tttggtgtaa aacgcgttcg actgaatacc gtaaaacagc
    19201 actgccagcg ataacgccac aaaagggatc tcaaaagtca tcgagatcag aatcaccagc
    19261 aggcagccta cccagagttg cacgatctgc ggcacgcggc cgggtcgccg ctcgcttaac
    19321 tcttcatgaa agaacgccag cagcctgacc accggtaatg gcagggagtt gagcgcgctc
    19381 attattgcgg ctcaagatta gcgactgccg aagcgccgat gcggaacatt tccgggtcag
    19441 gtttatcgac catgattttg accggaaaac gctgggcaac gcggacccag ttaatagagc
    19501 gagaaacttt cggcaggccg cccagcacca ggccgccgtc atccggtagc acgccgtagc
    19561 caatcgaatc cactttaccc tcgaaggttt tgccgctgtc actcatcagg cgaatcgttg
    19621 cgggtgtacc tgagcgaata tttttcagat cggtttcgcg gaagtttgcg atcacgtacc
    19681 agtgacgagt gtcgattagg gtaaaaatag ggcgcatggc agaagcaaat tgcccgacgg
    19741 aggttttgag ggaaatgacc cggccatcaa acggcgcgcg aacggtcgcc atttccagat
    19801 gaagtttggt cagggcaata tccgcttcga ccgccgcacg ttgcgccacc agcgcatcga
    19861 cgccgctgac ggcgctggcg gctgactgcg cctgtaacaa tacggcatta agatcggctt
    19921 ctgcggcgcg ctgcgcagtt cttgcacggt caacgtcttc cgctgagaca aaaccctctt
    19981 tcagtaatgg ctcggtgcgg cgtaatgtat ctgtggcctg tttcgcggcg gcacgggctt
    20041 tttctaccgt ggcattaatc gagtcggcac caaactgttg cgcatcaacg ctacgctggg
    20101 tgagcataat ttgcttatcc agcgccgcga gggaggcttc agctttcgcc agattggctt
    20161 cgtacgggcg cgggtcaatg cggaacagca aatcgccctg tttgactgcc tggttgtcgg
    20221 tgaccgccag ttctacaatg cggccgctga cttccggcac cacatcaatg gtatctgctg
    20281 acgcgtaagc gtcattagtt gatggcgcac tgtctacgcg ccagataacg aaaacaaggg
    20341 caaccagcgc caatgcaacc actaacagag cagggaattt actgcgagga gcttttttcg
    20401 gcgtactttc catgagcgcg tctcaaatag attagaaaaa tgccagccag aacagcatgg
    20461 cgtagagagc aaaaagggcg gtataaataa ttccgacaaa tgccagattg atatttgccc
    20521 gctgaataac tcgcctcgtg atgagcatca aaataaggct ggcaatggcg cagaaaaacc
    20581 agctgggata ataagcaccg atcaccggga ttgccggaga taatgagcat cccgaaacca
    20641 tgaagacggg aattatccag gctgtttttt tgagttgcat cgccatgcga gagagaactg
    20701 ttggcatatt aaatacatcc tgtctgctgc gaacaatatc tgtagtgaat atcaatatcc
    20761 attgaaggcg tcagtatggc tattttctcg atgaataaaa atagctt
//
LOCUS       DAJOWB010000081        20590 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJOWB010000081 DAJOWB010000000
VERSION     DAJOWB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.081"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(77..4537)
                     /gene="mukB"
                     /locus_tag="OHO85_003187"
     CDS             complement(77..4537)
                     /gene="mukB"
                     /locus_tag="OHO85_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCQ2672530.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTSDSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLECEREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="OHO85_003188"
     CDS             complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="OHO85_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCQ2672531.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="OHO85_003189"
     CDS             complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="OHO85_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCQ2672532.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="OHO85_003190"
     CDS             complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="OHO85_003190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCQ2672533.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDHVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7462..8241
                     /gene="elyC"
                     /locus_tag="OHO85_003191"
     CDS             7462..8241
                     /gene="elyC"
                     /locus_tag="OHO85_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCQ2672534.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8218..9111)
                     /locus_tag="OHO85_003192"
     CDS             complement(8218..9111)
                     /locus_tag="OHO85_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCQ2672535.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="OHO85_003193"
     CDS             complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="OHO85_003193"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCQ2672536.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIRNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAHEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="OHO85_003194"
     CDS             complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="OHO85_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCQ2672537.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10242..11474)
                     /locus_tag="OHO85_003195"
     CDS             complement(10242..11474)
                     /locus_tag="OHO85_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCQ2672538.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="OHO85_003196"
     CDS             complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="OHO85_003196"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCQ2672539.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="OHO85_003197"
     CDS             complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="OHO85_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCQ2672540.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVECGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14279..16543)
                     /locus_tag="OHO85_003198"
     CDS             complement(14279..16543)
                     /locus_tag="OHO85_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HCQ2672541.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPSLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ECWQWLSFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQLYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16750..17034)
                     /gene="ihfB"
                     /locus_tag="OHO85_003199"
     CDS             complement(16750..17034)
                     /gene="ihfB"
                     /locus_tag="OHO85_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCQ2672542.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17194..18867)
                     /gene="rpsA"
                     /locus_tag="OHO85_003200"
     CDS             complement(17194..18867)
                     /gene="rpsA"
                     /locus_tag="OHO85_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCQ2672543.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18978..19661)
                     /gene="cmk"
                     /locus_tag="OHO85_003201"
     CDS             complement(18978..19661)
                     /gene="cmk"
                     /locus_tag="OHO85_003201"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCQ2672544.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19834..20580)
                     /gene="ycaL"
                     /locus_tag="OHO85_003202"
     CDS             complement(19834..20580)
                     /gene="ycaL"
                     /locus_tag="OHO85_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCQ2672545.1"
                     /translation="MLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATLSDADAK
                     AIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSDVNAWAM
                     ANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDAISATSG
                     VASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLASL
                     DGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4923 a         5707 c         5034 g         4926 t
ORIGIN      
        1 ccgtaatgtg ccactttttt agtgcggaaa cggagttttc ggaaaaagaa aaggcggcat
       61 tgctgccgcc ttaattttaa ctcgcctgag aaggcgcttc gtcacttcct ggaagcgttt
      121 cagggagttg cggcgcaaat cctcgcaggc cgacgacgtg aacgtgttcg gtattctgga
      181 agactttacg caccagttta taggtggtgc ctttctccgg gctgatattt tccggcgctg
      241 cgatgatgag ctgcatttgc aaacgctcac acaattcaaa cagcgtggcg atagaacgag
      301 catccagtcg cgctgcttca tcgaggaaca gcaggcggca aggagagata tctttaccgc
      361 gcaggcggcg agattcatct tcccagcttt gtaccaccat caccagaatc gacataccgg
      421 taccaatcgc ctcaccggtc gacaatgcac cagactctgc gcgcaaccaa ccatcggaac
      481 cacggttaac ctcaacttcc atttccagat agttgcggta atccagcagt tcttcgccaa
      541 tggtctgcgg cgtgcgctgt cccatatcaa tctgcggatt aagacgctga tacaatttcg
      601 ccaacgcttc cgagaaggtc agacggttgc tgttaaacag atcctgatgc tgctcgtgct
      661 gttcagagag cacatccagc agcatggcgt gcgtttcacg cacgttcacg ttgaggcgca
      721 cgctgttcac ctgaccaaac gatacgttct gcaacccctg gttgagcata cggatacggt
      781 tctgctcgcg ctgaatggtt ttgcgaatga tgttcgccac gctgcgggaa ctgatcgcca
      841 gtttctgttc acgggaggtt aattcttcgg tcagacggct aagttcgatc tccatctgtt
      901 cgatagcttc caccggatca tcggtacgaa taatatcctg acgaatacgt tcacgcagat
      961 gctgataaac cgccacgaag aactgaattt tacgctccgg acgtttcgga tcttccgaca
     1021 tgcgcagcac gtcgcgcaga tgttcgttat ccgccaccgc cagacgcagc gcacccaatg
     1081 ccttatccga catagagcgc aaatcatcag cggagagata agccagctca cgacggtgta
     1141 agcggcgctc aacgccgtta tctttcacca tgcgcatcac cgcacaccag cccgctttgg
     1201 cggtcactac ctgctcgcgc atctcaaagt aatcccgctc cagcttgcgc agtttgcggg
     1261 tcaggttgtc catctccgct tcgcagaagg taagcgcttt ttccagttga ttgcggcgtg
     1321 aacggttatt gctcagttgc gcatgcagct cgtcacggcg aatacgcgcc cgctcttctg
     1381 ccccgctatc agcacgcacg ccgatatcct gcaattcacg ttgcagatcg ttgagtagct
     1441 cttttttggt gtcgtaagaa cttttcagcg aagccagcac ctggttgtac tgactcaact
     1501 gcgctgcgtg accacgcaac gcttcacgag cgcgagtacg ctccgcttcc gcctgttcca
     1561 gacgttcacg cagcttttcg ttgagatcgc tattaccgct cagcatttct gccgagtcag
     1621 aatagctaaa gtgcgcacga cgctgcacca cttccgtcag ggcaaacgcc tgctgacggg
     1681 catcgcgctg catctgctga gagtacgcgt aatcttcttt tagctgttcg aactgttccg
     1741 ggtcgctctg caataccgaa acgatcggtt ccagtttcgc cagttgattg ccaaactgct
     1801 gaacaaaacg cgcggcttcc tgggcttcat ccagacgttc gcggatttca tcgacgcgat
     1861 ccgccaggct gtcatcagcc aacaggttga gacgcggcag aatgcggttc agcgccgtaa
     1921 cgccttcttt cgcctgctca aactgaatac gctgctgctg gttatcattt tcatgattac
     1981 tcaacgcccg ctccagttcg acgcgacggc tgttcagttg acggatttct gcttccgggt
     2041 cagactcaaa cgcaaccgcc agatgactgc cgataaagcg gctgaacgcc tgatgcagac
     2101 gctgagtttt ctgtacatca aaggagagcg tggcgaagcg ttcggaaagc acttcacgct
     2161 cggcatggag gctttcaata cggctttcac gcgcagcacg accaaacagc ggcacttccg
     2221 ggaaacgtga ataacgccac tgacgatcgg cgattttcac cactaccgct ttttccagct
     2281 catcaacgct gaacacgctg tcatcgaatg actgcggatc tccttcgatc agatagagat
     2341 cttccgggca atcggtcaag ccttccaggt gttcagttac ctgtgacaga tctggcacca
     2401 cgatggcgtg gcgtgacggg ccatacagcg ctgagaagta cggcgcatct tccaggctaa
     2461 cgtcgtcata aatttctgac agcagcacac caccaaaacg ctccgccagc gcgttcagac
     2521 gctgatcttc agagccgcca ggctggctta aacgttcgat ctcttcatcg acggcgtttt
     2581 tgcgcgcgcc cacttcatcg cgttcaacaa tcgcctctcg ctcacactcc agcaactgtt
     2641 gcagatattc ggtgacgtcc tggctggagg taaactcttc gccgcactgt tcgctcaact
     2701 ggttgagact gttttgcgct gccagccaaa ccggcgcacg ctgcatcaaa ctctgaatgc
     2761 gagactgcag ctgttcctgt tcctggcgca gtgccatgcg ctcttcacgg gcgttagaca
     2821 cgctatcgga aagagaggca atgcgcgctt ccagctcctg atgcagagct tccagctcgt
     2881 caatgtcaaa attcttgccc tgacgtttgc agaaatctgc cagcagacgc tcagcttctt
     2941 gctgttcgcg cagacgctgt tccagttcgc ttaagcgcat ccgcaacggc tgaacctgct
     3001 ctgccaggtg acgctgatcg accccttcgc gcaatagttc gcgagcgaca tcccacgcct
     3061 cgttacgcgc cagcgggccg ttgattgcca ccaccagctg ataagcctgc tcaaactggc
     3121 tgtgcgcggt ttgcgccatg ctcattttct gctcaagaga gagcattttt tcagtcgctt
     3181 ccagctcttt cgcctggaag gtttccagcc attcggcggc gctgtcggag gttaagtccg
     3241 gcagatggca cagttctttg gcgcgattaa gcgcagcaat cgcctggtta tactggatcg
     3301 cgcgcgtttg ctggacgtcc agcgcctgct ggtagtcagc aagctggctt ttcagctcgt
     3361 ccacttccag ttcggcagct tccgcgcgag cctcgttctc ttcctggcgt tcgatggctt
     3421 ctgccaccac ttcgttttgc tcttccagac ggatctgcag ctcatcgaga tccgcttcgt
     3481 agcgttcgat cttctcctgc tgacgcagtg cggtttgcac caggttcagg tgatcgctgg
     3541 ccgcctgata atccgcttcc agatcgcctt cggcaccgtt gtgctctgcc agctcacgcg
     3601 ccatatcgac gtgtttgtac tgctcagccg ccagttgctg acgcgaagta tgtaactcgc
     3661 gacgaaactc cagggctttg tcgagatgga cacggcgctc gttggcgtga cgcatgtagt
     3721 ccgccgccac gtagttggta gcttcgctga tcagatgctt aaacaggtca cggtccgact
     3781 gggtgacacg aatcgcttcc agcgtcatgc ggttttcacg cagcgccgct tccatgtcct
     3841 ggaacgcttt acgcacgccg ctgttttccg gcaacaggta gtcgcgcaga gaacgggtaa
     3901 tggcactgga gatcccgcca tacagcgaag cttcgatcag acgatagaat ttgctacggt
     3961 cagatgcgga gcgcagacga cgcgcgatga tgccaagatc gaacatcagc gagtggtaat
     4021 cggtaatgga gttgaactgt ttaaactgca cgccttccat cgcctcgagc ttgtctttca
     4081 gctcgttaag cggcagcacg cgcgcctggc gttcgttcag ggtttcggtc accagctgtg
     4141 tcggctgcac cgacatcggc agtccctgaa tggcaaatgg tttgatatcc actttacgat
     4201 cgcgtccggc aacctgttgc agacgcacac cgaccaccac gcgctggtgg cgcgagttaa
     4261 tggtgtcgag catcgaataa cagacacccg ctttcagctt accgtgcaga cctttatcgc
     4321 gcgaaccgct ggtggccccg gcttccgtag tgttacggaa atgcagcagg gtcaggtcgg
     4381 ggatcagcgc cgtaacgaac gccgccatgg tggtggattt acccgccccg ttaccgccag
     4441 aaagcgtcgt gaccagctcg tcaaggtcaa aagttcgggc aaaaaagccg ttccagttaa
     4501 tcagcgtcag tgagcgaaat ttaccgcgtt caatcattat tcttcctctc cgctatctgg
     4561 ctgattctct tcggtttcat cgttgagttg cagatgattt tcaatcggca ttgcttcgcc
     4621 atcacgaatc aggcgacgct gtgcttcacg gggatcgtcg ccagcacgca catcggctcc
     4681 gaagcggaac accgattcgg taatgcgaaa cttgctgctg tcgtggccca taaaccacac
     4741 catgcctaaa cgacgcagac ggttgagcga agaacgtact ttctcctgca acttctgacg
     4801 gtcaacgtct gaaccggttg aacggttgtt caccagtttc agcagttttg cttcatcggc
     4861 cagggtgagc agttcgtcgt acagttcctg ctgggtgaaa atcccctcat tcgccagccg
     4921 ttccgggctg agatagagat aacagaggat tttcccgacc atcatatcca gttccgacaa
     4981 gacggaacga gggatcagcg tggtggaacg tgggcgtaaa tagaagaacc cttctggtgc
     5041 gcgaataagc tcaacgttat aacgcgcgta aaactcttcc agatattcct gaaaatccat
     5101 caggaatgca tgattatcca gttcgtcgag gccaatatgg cggcctgaac gtaaggcgct
     5161 gtccagcgcc ggaaataacg gattcgccag cgcctgcgcc agcttaaccg gcatcacttg
     5221 ttcaatattt gtcgatgaca tgcgcctgta ccttggctcc gtaatcatta atcggctgcc
     5281 atttcgctgg cagtccggtg aaatctgctt gcgctacgcc aagacgtacc gcctgatcaa
     5341 taacaatacg cgcaacgtca aagtgacgtg cacgcggata ctgtgacaga tattcgcgta
     5401 ccaccagacc aagatccagc ggcacttgtc tggttttgta cacggcaagt tgttcttcga
     5461 tgatcgccgc cagctgttcg cggatctcgt taaactcttc gtattccaga tcctcaggaa
     5521 gttccccagt cacttcttca tcgcgcagtg ccatctcttc gtcacgcata tccagcagac
     5581 gatcggcatt ggcataagtt agcgcccacg gctcatcaaa ataggtttgt accgactgac
     5641 gtaaccgctg agcaaagacg cggtttttat ccatatcgat cgcggtacga ataaatttgt
     5701 gtacgtggcg gtcgtagcca atccacaagt cgatggattg ctggccccaa ctgataatac
     5761 gatcgagttt gctctgcaga tcgaacacca gacgatcgac gaaatgcaga tcgtcatggg
     5821 tcatcgtcgc atcctgaatg cgcaacagat tagcctgcaa tttgtcgcct gccgcttcca
     5881 gcgtatcctg caattcacgc agcgttccgg aagtttccga aagcaacaat tcacagctgg
     5941 aaatcgccgc ccgccagtct ttgttcagca actgggcgat atcatccttc acctgctgct
     6001 gctgttcatc catcagacgt tgcgtcaggt cgatactgtc gaaaatttct gctaccgaat
     6061 atttcagtgg agcataaaca ttacggtgcc agtgaaattc atcaccgccc tcttcggcgg
     6121 catctgctgc gcgtttgagc tcacccgcca caatcgacaa ctgcatagaa aggcgcagcg
     6181 tagaaaactc gcgctgacga atgtaatagt cagtgatgcc gatgccgagc ggcgtcagac
     6241 ggtaaattgc gttcccttcc gcctgctcgc tggtaaagcg gttcagcaga cgttgacgca
     6301 ccatgtcgtt gatcgcgttg ttggcgcgca cgccgatggt ttcgctggtt tgctcaaacg
     6361 catcactcac atggcggaat gcatccacca gctcgccttc actcatctca ccatccagac
     6421 gctcgccgtt cagcgtggca accgccagca gaaaagagag tcggtctacc ggcagcgaga
     6481 tggagaagtc attttttctg gcccaggcaa ccagttcggg gactgtctgg gaaaattcac
     6541 tcatacttta tccttgctct gcggtttgcg cgcggtgaca tgaatataac gccccagggt
     6601 aatatacggt tcctgacggc aataacgcgt ttctaattca agtaatgctt catagcagtc
     6661 gcgctgctgg tgcttctcgc gcagataatc atgaaacacg cgaacgcctg tcttacccat
     6721 aatttgccaa ccagcttctt ccagccacag ataaacctgc gccgggtcgc gtggataatc
     6781 tggcgaaagc gtccgttttt tctttttcgg cattcccgcc tgcacgtaat caaaattccc
     6841 ggcgaccatg ttatgcatca acaaaccatg cgcattgtag aacattaacg acaacactcc
     6901 gcctggacgc aacactgacc acagggtctg caatacactg cggggatcgg ccacccactc
     6961 gagcaccgca tggaacaata tcagatcaac gggcgtttcc aaatgcgaag caacatcctg
     7021 agcggcgcaa tgtataaatt gcatgttgtc gctcacacct tttgcttctg ccgcctgttt
     7081 tgcgcggtcg atcatctgcg cagaaagatc gcataaaatg acctgatgcc cacgctcggc
     7141 cattttgatt gcggtctgcc cttctccacc gccagcatcc agcacacgca gtttttgcgg
     7201 gcccatttcc gccagcacgt gatcgagatc ctgccacaga atagcctgtc gaagctgccc
     7261 tttggtggtg ccgtaaatgt tacgggaaaa cttttccgca atatcatcga aattgcgatc
     7321 ctgcattgct ttctccaccg gttggctaag acgttttctg ataaagacgc tattttgtca
     7381 cagccacggc gagaatgaac ccgtctggtg taatatcgct ggcgatccct tgctatatgg
     7441 ttaaaaaagg aaccagaaag gatgcttttt acactgaaaa aagtgattgg caatatgttg
     7501 ctgccacttc cgctgatgtt gttaattatt ggcgctggcc tggcgctgct gtggtttagc
     7561 cgttttcaaa aaaccgggaa gatttttatt agcattgggt ggctggcact cttgttattg
     7621 agtttgcaac cggtagccga ccgcctgctg cgtcccatcg aaagcaccta tccgacatgg
     7681 aataattcgc aaaaagtgga ctatatcgtg gtgctcggag gtggttatac ctggaaccca
     7741 cagtgggcac cgagctctaa tttaatcaat aacagtctgc cacgcctgaa tgaaggtatt
     7801 cgcttatggc gcgaaaatcc gggatcaaaa ctgatcttca cgggaggcgt agcaaaaacc
     7861 aatacggtga gtacagcgga agtaggtgcc agagttgcgc aatcgctggg cgtgccgcgc
     7921 gagcaaatta tcaccctgga tttgccaaaa gataccgaag aagaagctgc ggcagtgaaa
     7981 caggcgattg gtgacgcccc tttcctgctg gtgacctccg catcacatct gccgcgggcg
     8041 atgatctttt ttcagcagga agggttaaat ccgctaccag ccccggcgaa tcagttggcc
     8101 atcgactcgc cgcttaatcc gtgggaacgg gcaatccctt ctccggtctg gctgatgcat
     8161 agcgatcgtg ttggctatga aacgcttggc cgcatctggc agtggctgaa aggtccgtca
     8221 ggcgagccac ggcaagagtg attttgatgc cagatcgaag ttgcgccgac taaatcgtcc
     8281 ggtattaacc agttgcgcaa cttcatccca taacacatac aaccaacgcc gccagatgaa
     8341 cgactccgcc actggcgcgc gttgcaggta actccagagc aaatcttccg ccagagaatt
     8401 atccattagt cggaacagtt cgtattctcg cggtgcccaa agcattagtc ccggcccgac
     8461 catcgccagt aactgatcgc tgcgcgaatc tttcagcatg ctgcgtaaac agaagttacc
     8521 gtgaatcaac acacaattgt cgttaaagcc ttcaaataat gccgggagac attcgcgagt
     8581 gcgaaacagg atccgcttat cctgcatcgt cagaccggtg ttattgaact gattgagcgt
     8641 ggtccatagc acttcaacat gttgccggta ccatgagggc cagaaatttt cctgagtatt
     8701 gtcgaccgcg ccgacgcaac cgcgactgtc ctgacggtgc caggccagta aggcttcaac
     8761 gatttggtct ttgagttgtt cccagcgttc tggtgttcgg gctggtgcct ccaccgaaac
     8821 accacgcatc cgctccagta gcaggacatc ggggccgggg tgttcttcat gcgtcatcac
     8881 accataaata gtcggcatac ggacagtccc acttcttgcc agcatggtgg ttttccatgc
     8941 cagttgtcgg gcttttccgg gcgtactaaa gcttcttgcc attaacggca ttgggtttcc
     9001 ctggctgtca tacaaagccc acaacgccgt atccgctttt tcattgacgc actcaatacg
     9061 actgagtttt tcgcccagta aatggcttaa ttcggcacgc agctgttcca ttcagattac
     9121 cctcataaaa gctactgctt tcataatgag cgccaatata aaagatgtca cctgaatgag
     9181 atcaattgtt gaaaagaaaa attgaggaaa atcgtaacgg tacgacactc ctcccaaaat
     9241 tggctgaagt gtcgtgaagt gaaattagcg catttcagcg cgaacgcgct cgagatcttc
     9301 aggggtatcc acacctgtgc caggaacttc atgcgcaaca gcaacatgga ttttttcgcc
     9361 gtaccacaga acacgaagct gctctaacat ttcgatgtgt tctaacggac ttgcctgcca
     9421 gttgacgtaa cgacggataa agcctgcacg gtagccataa ataccaagat gacgcaggaa
     9481 gttatcgcca acggtttcaa ggccttctgc aaaacgatca cgatcccaag gaatggtggc
     9541 gcgtgagaag tacagtgcat acccttcagc gtcgagaacc actttcaccg cattcgggtt
     9601 aaacgcttct tccgcattgc ggattggcac cgccagagtc gccataccca cctgacgctg
     9661 agcgaggtta tcagcaacct gacgaatgat tgtcgcaggg atcatcggtt catcaccctg
     9721 cacattaacg atcaccgtgt cgtcgctgaa tgcgcatttt tcgacaactt ccgccagacg
     9781 ttctgttcct gactgatgat cggcgcgcgt catacatact tcaccgccag cggcttcaac
     9841 ggcgcgggca acatcctcat gatcggttgc cacgatgatg cgctcggcac ctgattcacg
     9901 cgcgcgttca agaacatgaa caatcatggg tttgccgtta atatcaacca atggtttacc
     9961 gggcagacgc gtggacgcgt agcgcgcggg aataatgacc acaaaactca tgatttactc
    10021 tcatcagcag tcagcacgcg ggcttccgtt tccagcaaca ctggaatgcc atcgcggagc
    10081 gggaaagcca ggttatccag tttgcaaata agctcttgtt tttcctggtt ataccaaagt
    10141 tttccgttgc aaaccgggca ggcaatgatt tcaagcaaac gatgatccat agttcctcca
    10201 gatggatcgg gttatgaatg cataaatctt atcataatca tttatgcgac ggggtctatt
    10261 tcccagccag tgcggcaatc agtaaagaga ccttgcgggc agcgtcctaa tgtcacccgc
    10321 gttgcctgct gccagctcgc gaaatcagta atcgcctgac gtaacccttt ttgcagcatt
    10381 gtcgttggtt taatgccttc ctgtaaccac agagagataa cttcaaggat gcctgtctgg
    10441 cgatgcattt tggcatccat tcgcccaact aattgcccac gatgtaataa tggcagaaca
    10501 aaatagccat actggcgttt cggcgctggg gtatagcact ccagccggta gctaaaatca
    10561 aaaagctgct ctgcgcgttt gcgatcccag acaacaggat caaaaggcga aagtaccgcg
    10621 ctgtgcgttg cagtgagctt tccggccagc gcacgctcga gtagcggcag caaatcagca
    10681 tgcagccaaa gattgcccaa tttttcaaca tgcacagcaa tgatttgctg ctgttcagcc
    10741 ctcgcttcgc gccacgccgc cagtgccgga cgtttcagcc gatagtaatc tgccagccac
    10801 tgttcgcgga atattcccag gctacgcgca ctgttatcca gcatgatgat ttctgcttct
    10861 gtttgcgaaa cgagatcgcg ctcatcatcc cagtcaggca tgacacggtg ggttaaatca
    10921 taaacgcgct ggaagttgcg ccgttcaatc accatcacct ttccggcagt aaataaacct
    10981 tccagatgcc gtttatgcgg cttccattcc caccagccgc ttgcaccttt acgaggatgc
    11041 tcaaaatcgg ctgaacgtac cggcccctta tcatgaatat gctgaattaa ctgtgcaatt
    11101 tccgcctcat gttcctgcat ccaggcgtct ttgtatttcc agcccatttt ttcaggtgcc
    11161 agcatgcggt ggcgaataag acgaaagtcg ctacgcggca taaagcaggc ttcatgcgcc
    11221 cagtattcca ttaattcgcc acgcgccaga gactcatcca gccactgggc aggataattt
    11281 cccagacgac tgaaaagcac cagatatgga ctacgggcaa caatattgat ggtatcgatt
    11341 tgcagcaagg acatgcggga gatcgttgcc ggaatatcct ccaacgacgc tcgacggcgg
    11401 ggtttgttta acaggccttg tgcggcaagg tgaagattac gcgcatcagc aagggagagg
    11461 tgcggcagcg acattcatga ctccatcaat cgaacgctgc cgcggcgtaa ctagttgcca
    11521 gaagccagcg aggttagttg cgtaagcagt ttcgctggtt catcacctga aagctgtgcg
    11581 tctacaggca aataccacca attttcttct gcaaaggccc ggcatttcac cgcatctttt
    11641 tcagtcatta ccagcgtttg cccggcgctt accaacgcac tgacatccgc atggttcaaa
    11701 gactgatgat cggccagcgg tacacatttt tccggttgta cgccacacat cttcagcgtg
    11761 gcaaaaaagc gcggcggatg cccaatcccc gccatcgcca ctacatgttc aagctgagca
    11821 acgtcacaac gcgtaccggt acgtaaattc accgcctgac ccggcagcag atgcatgggg
    11881 atttcaccgc tgcgagggac accgccgttg acgattaccg catcaaccga ctttaagcgc
    11941 cccgctcgct cacgcattgg ccccgccggc aaccaccagc cattgccaaa gcgacgcaca
    12001 ccatcaataa cgacaatttc cacatcacgc gccagacggt aatgctgtaa accgtcgtcg
    12061 gttacgatga tctgcacatc agggtgttgc gccagaatgg cttttaccgc atcagaacga
    12121 acgggagaaa ccgcaacagg cgcatcagtg cgttgataaa tcaacacagg ttcatcaccc
    12181 gcctgtgctg ttgtggtatc tgccgacaat aacagcggat aagattcagc cttaccacca
    12241 tatccccgcg atacgacccc cacgcgaata ccgcgctgtt gcaactgttc caccagccag
    12301 acaacgaccg gggtttttcc gttgccgcct gcggtgagat taccaaccac gacaaccggt
    12361 acgggggcac gccaggcgcg cttcagtttt agtttatagc aaagacggat cgcgccactc
    12421 accaggccat acaaccagga gagtggcagc aatagccgcc acaaagggga ttcaccagac
    12481 cagatttttt cgatcattgg ccaaactgca ttttgtgaag ttgcgcgtaa acgccgcggt
    12541 gctcaagcaa atcgttatgc gtaccgcatt ccacaatgac accatcctcg acgaccacga
    12601 tttcgtctgc cttttcaatg gtagacaagc ggtgggcaat caccagagag gtacggtttt
    12661 tctgcaactc atccagtgcc gcctgaatcg cacgttcgga ttcggtatcc agagccgagg
    12721 tagcttcgtc cagaatcaga atcgggctat cacgcaacaa ggctcgagcg atagcaatac
    12781 gctgacgctg accgccagag agcagcacgc cgttttcacc aatcactgta tcgagaccgt
    12841 tatccatctt attgatgaag tccatggcgt aggccatacg cgccgcttct tcaatttgct
    12901 cacggctgta ctgttcagtc cgtgcgtaag caatgttgtt agcaaccgta tcgttaaaca
    12961 gatggacatt ctgcgacacc agagcaacct ggttacgtaa cgacgccagg gtatactcgc
    13021 gcagatcgtg accatccatc aggatttcgc cttcatcaat atcgtaaaaa cgcgtgatca
    13081 ggctggcgat ggttgattta cccgaaccag agcgtccaac cagagcaacc gtcttccctg
    13141 ccggaatttt caggttgatg ttacgcaatg caggtacgtc acgtcccgga taagtaaagg
    13201 tgacattgcg gaattccacg tcgccagtcg cacgctcgat cacgcgctta ccttcatctt
    13261 tctcctgctc actgtccaga atggtaaaca gcgtctgaca agccgccata ccgcgctgga
    13321 actgggcgtt aacgttggtc agcgatttca gcggacgcat cagtgcaatc attgaagaga
    13381 aaacaacggt aatcgtaccg gcagtcaggc tatccatgac acttgggaag ctcgccgcat
    13441 acagaacaaa cgccagcgcc aaagaggcga tcagctgaat gatcggatca gagatggaag
    13501 aggctgaaac cattttcatc ccctgaagac gcattcggtt gctgacttta tcaaagcgtt
    13561 tcgtttccac ttcctgacca ccgaaaatca atacttcttt gtggcccttc agcatttgtt
    13621 cggcgctggt ggtcacctgc cccatggtgt tctgcatgtt tttactgatg ttgcgaaaac
    13681 gcttcgatac tacgcgaatc gcaatcgaaa caatcggtgc cagcacaatc aaaatgatcg
    13741 acagttgcca actgtaatag aacatcatga tgaacaggcc gatgattgac gcaccttcac
    13801 gcacaaccgt tatcagtgcg ccggaagaag aagaagcaac ctgttcggaa tcgtaggtga
    13861 tacgtgacaa cagcgtcccc gttgactgtt tgtcaaaaaa tgaaaccggc attcccatca
    13921 tgtgaccaaa caggcggcga cgcatggtca ttaccacctt tcctgatacc caggagatac
    13981 agtagctgga gacatagctg gtgataccac gtaaaatcat cagcccgatc accaccagcg
    14041 gcatccacac cagcacggag cgatctgttt taccaaagcc atcatcaaga agtggcttaa
    14101 ggagcgataa catgaaggta tcgctggctg cgttgaggat taacgctacg cccgccacga
    14161 tcagacccgc tttaaaaggc gcaatggttg gccacagtcg gcggaacgtc tgccacgtag
    14221 agagatcttt gtcgttatgc attcaaaaaa ccagcatttg ttgaaatagc cgcatattct
    14281 acccgttatc cactggcacg ccaaaccact gatgatacca acgaggtaaa atttgctctc
    14341 tgaggctgct aatccgccag ccttgcgctg aaaaattgac cgtcacttga ccctgatgtg
    14401 gagtatcaag ccattgatat ccttgctgtt gatagcgatg cttaacttta ttagagggca
    14461 atcgccatgc gttatagcgc gatgccgatg cgagtgccac ttttccattc actcgctgaa
    14521 ttaatggcaa cgatgatgag gtattactgc catggtgagg tacctgaagc aatgttgtct
    14581 gcacttgctg ccagtaacgg cttagcatct tttgttcagc ggggacttca atatcaccgg
    14641 ttagaagaat gctattcgtc ccgtcatcaa ccttaaccac acaggaatgg ttatttcctt
    14701 tatcgttgct agcttgtaaa ggccagtgca cgctgaaacg caatccttgc cattgccacg
    14761 cttcgccacg cacacagggc tgatgatgtt cccagtttaa cggacttctg atccataaca
    14821 tcggccatgt gtgcaatatt gagtccagcc ctccccggtg atccagatgt tcatgactca
    14881 gaataacgcc ttccggttca agattatgcc agtggagcca ggggataatc agttgttgcc
    14941 cactatcccc ttcgggccag gccagtcctg tgtcatagag aatcgctttg ccgtttctgg
    15001 ctatcaccat tgccagccct tgcccgacat caagcatgta cagctgccac tcgtcaggtc
    15061 gaggtttttg ccacagcggc cagcacatca gcaagcctcc agccacacac attgctggca
    15121 acgttcgcca ggcgtttaat cgccatacca ccagtaagaa ccatggggaa aatgatagcc
    15181 attgccaaca ttcagcaatg ttgatccacc cttccggcaa actctttaac ccccagaaaa
    15241 gtaaagccaa agaccggtcg gcaagaaacc ataacccttg ctccaggatt aacggcccgc
    15301 ttaaatgcac aaccatcgcg gcgaggatca acggaaccgt gataaatgtc accaggggaa
    15361 ttgctaatag atttgcaata aacgaggtca gactaatgcc atgaaatatg acgatttgca
    15421 cgggcataag cagaagtgtg attcccagtt gcagatggat gagggaaaca actgcacgca
    15481 ataccggcgg cagttgccac tcaggacagg gaaaccactg ataccaaaat atcaatgccg
    15541 cgaccgcagc ggcagagagc cataaacttt gcgagagaat ggcaacagga tccatcagca
    15601 aaattgccgc cagacaacat atccatacat cccagccact ccactgtcgc ccacttaact
    15661 taagcattcc ccacgtagca agcgccacaa cggtacgcaa tgcaggaggt tgcattcccg
    15721 tcagccaggc ataaaaagca gcacagcaga ttccgccaat taatggcatt tgccagtgga
    15781 tccagcgccc aggcagaaaa atttgtccac tgcgaatgag tccggcagcc agcaacgccg
    15841 caaaagcgat gtgcaatccc gatatcgcca ttaaatgcgc cgttccagta tcacgcatga
    15901 tatttttgat ttctttaggg acggataacc gttcccccat acctaaacca agaataaccg
    15961 cattccacgg atagggttgc agcgttgttt gtagtgacgc cagatactgt gcacgcaggc
    16021 tacaattcgg ttcaataaca cttgcctgca gaaagcggcc ggtgagcggc tgatgctggg
    16081 caatggcata acgctggcta tcaaagccgc catcattaag ttgaccatga acggcacgaa
    16141 ctttgagttt cattgaccac agttgtccgg cacaaaccgc ttgcggaaga tattcgccat
    16201 acagcacaag gcttggcgca gggaatatac gtcgaccttg tagatgagta atttgaccgt
    16261 aatgagtggt cataccgtca gtagcagtga tctcaacaat tgcatcctgc gtcgcgccag
    16321 ttaaggtttc tcctgcccac aaaatttgct ttgctgctaa tatgccccaa acgaaaaaca
    16381 acaacgtcag cgcgatataa cggacggttt taacagggat aaatgccagt acgcaggcga
    16441 agagagtcag aaacgcaagg gttaatgtcc caggcaattg gggcaaaatc agcaacggaa
    16501 aaattccgca aattatgcat acgccgactg tcgttatttt catgcacccc tccctgttta
    16561 tggaaagtgt gcaactttgt tgagcggttg tctggtggga attttgcgtt ccccgaggca
    16621 cgttcagaag aatttaacga gagggtgaaa actgctcaaa actctgcgag gcagcttcca
    16681 gttcctgatt agagaaatga aaaaagcacc cgacaggtgc ttttctcgcg ttcaagtttg
    16741 agtaaaaact taaccgtaaa tattggcgcg atcgcgcagt tctttacctg gtttaaagtg
    16801 aggaacgtat tttccttcca gttctacttt atcgccagtc ttcggattac gtccggtacg
    16861 tggtgcgcgg tagtgcaaag agaaactgcc gaaaccgcgg atttcaatac gctcgccctg
    16921 cgcaagagtc gaggccatat gctccagcat ctcttttact gcatcttcaa ccgtcttggc
    16981 gggaatgtgc gattgctggg tggcaagtct ttctatcaat tctgacttgg tcatgattcc
    17041 tccggttcct taaaggcaaa ttaagcggct gctgttgctg cattgattaa gggcggccgt
    17101 agccgccctt agtgcttgat tacaggacga aacctgcaat ctgtcaagta aactcaacaa
    17161 acttcggaat aaaaatcccg aagagtcaga gaattactcg cctttagctg ctttgaaagc
    17221 ttcagccatt gcgttgttgg agaagtttgc atcttcctgt ttgttaacag ttgcgattgc
    17281 atctttctcg tcagcttcgt ctttcgcacg aacagacagg ctgattgcgc ggtttttacg
    17341 atcaacgccg gtgaatttag cttcaacttc gtcgccaacg ctcagaacca gggtagcgtc
    17401 ttcaacgcgg tcacgggatg cttcagaagc acgcaggtaa ccttcaacgc cgtcagccag
    17461 ttctacggtt gcgcctttag cgtcaactgc agttacttta ccggttacga tagcgccttt
    17521 cttgttcaga gcaacccagt tgttgaacgg atcttctgcg agctgtttaa cgcccaggga
    17581 gatacgttca cgttctgcgt caacctgcag aacaactgca gcgatttcgt cgcctttttt
    17641 gtattcacga actgcttctt cgcctgcaac gttccaggag atgtcagaca ggtgaaccag
    17701 gccgtcgatg ccgccgtcca ggccgatgaa gataccgaag tcagtgatag acttgatttt
    17761 accttcaaca cggtcgccct tgttgtgggt ttccgcgaac tgctgccacg ggttagcttt
    17821 gcactgtttc agacccaggg agatacgacg acgttcttcg tcgatatcca gaaccataac
    17881 ttccactaca tcgccaacgt taacaacttt ggacgggtgg atgtttttgt tggtccaatc
    17941 catttcggaa acgtgtacca ggccttcaac gccttcttcg atttcaacga agcagccgta
    18001 gtcggtcagg ttggtcacgc gaccagtcag tttggtacct tccggataac gtttagcgat
    18061 agctacccac ggatcttcgc ccagctgttt caggcccagg gatacacggg tacgttcgcg
    18121 gtcgaacttc agcactttaa cagtgatttc gtcgcccacg ttgacgattt cgctcggatg
    18181 cttaacgcgt ttccaggcca tgtcagtgat gtgcagcagg ccgtcaacgc cgcccagatc
    18241 aacgaatgca ccgtagtcag tgaggttctt aacgatacct ttaacttcca tgccttcctg
    18301 caggttttcc agcagctgat cgcgctctgc gctgttttcg gattcgataa cggcacgacg
    18361 agaaacaaca acgttgttgc gcttctgatc cagcttgatt actttaaatt caagctcttt
    18421 gccttccagg tgcagagtgt cacgcaccgg acgaacgtct accagagaac ctggcaggaa
    18481 cgcacgaata ccgttcagct caacagtgaa gccgccctta actttgccgt tgataacacc
    18541 ggtaacagtt tcagcatctt cgtaagcttt ttccagcgtg atccaggctt cgtgacgttt
    18601 agctttctca cgggacagca gagtttcacc gaagccgtct tctactgcgt ccagagcaac
    18661 gtcaacttcg tcacctacct ggatttccag ctcgccctgg gcgtttttga actgctcagc
    18721 cgggatggcg gactcagatt tcagaccagc gtcaaccagt actacgtctt tgtcgatagc
    18781 aacaacaacg ccacgaacga tagaacccgg gcgggtttcg atttctttta aggactcttc
    18841 aaagagttga gcaaaagatt cagtcatgtt taatcttcag gtttatattt aacgtccacc
    18901 tggctccatg ccggatgggg ttgtttaaca tacccgctgt cattccattg caacgggggt
    18961 actgcaaatt cggtcgctta tgcgagagcc aatttctggc gcgcgtattg tagcgctttt
    19021 tcaatcactt gctcaatgct taaggtggtg gaatccaaca ctaaagcatc ggctgccgga
    19081 accagtggcg ctaccgctcg gttacgatcg cggtcgtcgc gttctttgat ctcggccaaa
    19141 aggcgctcaa agttaacact aaagcccttc tcctgcaact gtagcatgcg gcgatgcgca
    19201 cgttcttccg aggaggcgtc aaggaaaatt ttcactggcg catcagggaa taccaccgtt
    19261 cccatgtcgc ggccatcggc aatcaggcct ggtaattcgc gaaacgcgcg ttggcgacgc
    19321 aataatgctt cacgaacgcg tgggaatgcc gcgacttgtg aagctgcatt cgccacttcc
    19381 tgagtacgaa tttcgccgct gacatcttcc ccttcgagga tcacttccag attgccattg
    19441 gttgacacaa aacgtacatc cagatgggat gccagcggta ccagcgcatc ttctgacgca
    19501 acatcaacat ggtgatgtaa tgccgccaat gccagtacgc gataaattgc acccgaatcc
    19561 agcagatgcc attgcaatgc ttccgccata gccttacaca aggtgccttt ccctgcaccg
    19621 cttgggccat caatggtaat aaccggggca attgccgtca tctttatctc cttaaaacag
    19681 gcgtaccgtt aacataaccg cgcgcattat acgcgccgac gccacaaatc gttaacgttg
    19741 tgtgcaaaaa ccagttttgt ctgtattcag atggggattt attgcgcaat tatagcgggc
    19801 tggcagaaga ccagcccgaa agatgacaat gatttactta ccagaggcga tacgatcacg
    19861 gatgtgttgc gcacgctctg ttgatggtgg gtgagagtca aacatggact gggtgcgacc
    19921 gccatccaga ctagccagtt tttcaaagct gccaaccagc ccctgggtgc tgatgccacg
    19981 tttcttcaac agatcaaagg agaaatcatc tgcttcggac tctttatcac gggagtattt
    20041 agcattaata gcgccttctg cgatatcacc caattgtgag cgggaaagct gggaagccac
    20101 tccgctggta gctgaaatgg catcgcgtgc ggcaacgatc gcataagaag ctttcatttc
    20161 agccagcgag tgacccaacg cgacgtggcc cagttcatgg cccagaacac cttcaatttc
    20221 gttgtcattc atcatgtcca tcaggccact gtagacacgg acacagccgt tcgccatcgc
    20281 ccatgcgttg acgtcgctgg tcatataaac cttatagttg accggcgtgc cattaatatt
    20341 gttacccaat gctttggcga tttttgccag acgtttaccg tacttgctgg atttacttgc
    20401 gacttgattg ccgctgtcca tttcggcaca gccctgattg gcaatcgctt ttgcatcggc
    20461 atcgcttaat gttgctgctt tataggcatt taaaccggag ctgatagcca tattggtatc
    20521 aataccgtgg gtattttgac accctgtcag taatgctgca gaggtcgcaa tcgccagcag
    20581 taatttagta
//
LOCUS       DAJOWB010000082        20463 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJOWB010000082 DAJOWB010000000
VERSION     DAJOWB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.082"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            212..631
                     /locus_tag="OHO85_003203"
     CDS             212..631
                     /locus_tag="OHO85_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCQ2672546.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            631..1899
                     /gene="umuC"
                     /locus_tag="OHO85_003204"
     CDS             631..1899
                     /gene="umuC"
                     /locus_tag="OHO85_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCQ2672547.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALSVDEVWGIGRRNSKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1945..2475)
                     /gene="dsbB"
                     /locus_tag="OHO85_003205"
     CDS             complement(1945..2475)
                     /gene="dsbB"
                     /locus_tag="OHO85_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCQ2672548.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2621..4162)
                     /gene="nhaB"
                     /locus_tag="OHO85_003206"
     CDS             complement(2621..4162)
                     /gene="nhaB"
                     /locus_tag="OHO85_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCQ2672549.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4384..5103
                     /gene="fadR"
                     /locus_tag="OHO85_003207"
     CDS             4384..5103
                     /gene="fadR"
                     /locus_tag="OHO85_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCQ2672550.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5155..6687)
                     /locus_tag="OHO85_003208"
     CDS             complement(5155..6687)
                     /locus_tag="OHO85_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCQ2672551.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7017..8315
                     /gene="dadA"
                     /locus_tag="OHO85_003209"
     CDS             7017..8315
                     /gene="dadA"
                     /locus_tag="OHO85_003209"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCQ2672552.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8325..9395
                     /gene="dadX"
                     /locus_tag="OHO85_003210"
     CDS             8325..9395
                     /gene="dadX"
                     /locus_tag="OHO85_003210"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCQ2672553.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9781..11517)
                     /locus_tag="OHO85_003211"
     CDS             complement(9781..11517)
                     /locus_tag="OHO85_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCQ2672554.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11612..12526)
                     /gene="ldcA"
                     /locus_tag="OHO85_003212"
     CDS             complement(11612..12526)
                     /gene="ldcA"
                     /locus_tag="OHO85_003212"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCQ2672555.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIHRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12626..13237
                     /gene="emtA"
                     /locus_tag="OHO85_003213"
     CDS             12626..13237
                     /gene="emtA"
                     /locus_tag="OHO85_003213"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCQ2672556.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13239..13973)
                     /locus_tag="OHO85_003214"
     CDS             complement(13239..13973)
                     /locus_tag="OHO85_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCQ2672557.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14174..14428
                     /locus_tag="OHO85_003215"
     CDS             14174..14428
                     /locus_tag="OHO85_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCQ2672558.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            14606..15046
                     /locus_tag="OHO85_003216"
     CDS             14606..15046
                     /locus_tag="OHO85_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672559.1"
                     /translation="MIQKINILDISQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15125..16822)
                     /gene="treA"
                     /locus_tag="OHO85_003217"
     CDS             complement(15125..16822)
                     /gene="treA"
                     /locus_tag="OHO85_003217"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCQ2672560.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSYKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17142..18560)
                     /gene="dhaM"
                     /locus_tag="OHO85_003218"
     CDS             complement(17142..18560)
                     /gene="dhaM"
                     /locus_tag="OHO85_003218"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCQ2672561.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLLSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELEIGWICQQGEKL
                     YAIQPEEKLTLDVKTQRFNRQG"
     gene            complement(18571..19203)
                     /gene="dhaL"
                     /locus_tag="OHO85_003219"
     CDS             complement(18571..19203)
                     /gene="dhaL"
                     /locus_tag="OHO85_003219"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCQ2672562.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESGYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPAALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19214..20284)
                     /gene="dhaK"
                     /locus_tag="OHO85_003220"
     CDS             complement(19214..20284)
                     /gene="dhaK"
                     /locus_tag="OHO85_003220"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCQ2672563.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQEPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQQLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
BASE COUNT         5087 a         5117 c         5298 g         4961 t
ORIGIN      
        1 tggcgaatgc ttctattcta ttttttagcc gggtgatatt tttcatttct gctggatgcg
       61 tcgtcgccag aaggccacgt gagcacagga taagagaacg aaaaatcagc agtctatgca
      121 gcgacaaata ttgatagcct gaatcagtat tgatctgctg gcaagaacag actactgtat
      181 ataaaaacag tataacttca ggcagattat tatgttgttt atcaagcctg cggatctccg
      241 cgaaattgtg acttttccgc tatttagcga tcttgttcag tgtggctttc cttcaccggc
      301 cgcagattac gttgaacagc gtatcgatct gaatcaactg ttgatccagc atcccagcgc
      361 gacttatttc gtcaaagcaa gtggtgattc tatgattgat ggtgggatta gtgacggtga
      421 tttactgatt gtcgatagcg ctattaccgc cagccatggt gatattgtca tcgctgctgt
      481 tgacggcgag tttacggtga aaaaattgca actacgcccg acggtacagc ttattcccat
      541 gaacagcgcg tactcgccca ttaccatcag tagtgaagat acgctggatg tctttggtgt
      601 ggtgatccac gtcgtaaagg cgatgcgctg atgtttgccc tctgtgatgt aaacgcgttt
      661 tatgccagct gtgagacggt gtttcgccct gatttatggg gtaaaccggt ggttgtgcta
      721 tcgaataatg acggttgcgt tatcgcccga aacgctgagg caaaggcgct tggcgttaaa
      781 atgggcgatc cctggttcaa acaaaaagat ctgtttcgtc gctgtggcgt ggtttgcttt
      841 agcagcaatt atgagcttta cgcagacatg agcaatcggg tgatgtcgac gctggaagaa
      901 ttatcgcccc gcgtcgagat ttacagtatt gatgaggcat tctgcgatct gacaggtgtg
      961 cgtaattgtc gcgatctgac tgattttggc agagaaattc gcgcaacggt gctacaacgt
     1021 acccatctta ctgttggtgt ggggatcgcc cagaccaaaa cgctggctaa gcttgccaat
     1081 catgcggcaa aaaaatggca gcggcagacg ggtggggtgg tggatttatc aaatctggaa
     1141 cgccagcgta aattaatgtc tgctctctcc gtggatgaag tctgggggat tggacggcgg
     1201 aacagcaaaa aactggacgc gatggggatc aaaaccgttc tcgatttggc ggatacagat
     1261 atccggttta tccgtaaaca ttttaatgtc gtgctcgaaa gaacggtgcg tgaactgcgc
     1321 ggcgaaccct gtttgcaact ggaagagttt gcaccgacga agcaggaaat tatctgttcc
     1381 cgctcgtttg gtgaacgcat cacggattat acgtcgatgc ggcaggccat ttgtagttac
     1441 gctgcccggg cggcggaaaa acttcgcagc gagcatcaat attgtcggtt tatctccaca
     1501 tttattaaga cgtcaccatt tgcgctcaat gaaccttatt acggcaatag cgcgtcggta
     1561 aaactgctga cgcccactca ggacagcagg gatatcatta acgccgctac gcgatctctg
     1621 gatgccatct ggcaagcagg ccatcgctac caaaaagcgg gcgtgatgct gggggatttc
     1681 ttcagtcagg gagtcgcgca gctcaattta ttcgatgaca acgcaccgcg ccccgggagt
     1741 gagcaattga tggcggtaat ggatacactg aatgctaaag agggcagagg aacactctat
     1801 tttgccgggc aggggatcca gcaacaatgg cagatgaagc gagccatgct ttcaccacgt
     1861 tatacaacgc gaagttctga tttactgcgg gtcaaataaa catagcggca ggaaaaaagc
     1921 gctcccgcag gagcgccgaa tggattagcg accgaacagg tcacgttttt tcgctttaaa
     1981 cggctgggaa atcaccacca gcaccgcgac aatcaggtaa gcgataaaaa taccgagcag
     2041 ccactgcggc atttccagac ctaaaaaatc ccactgacgc tcggcacaat cgccagaggc
     2101 gacaaacact tgcggcaccc atttatccag cggcagccat tccgggaaac gaaccataaa
     2161 atcacaggtg gcaaacggcg aaggatagag ctgaagcatg gtgtgctcgt atgttaactg
     2221 cacaccgcgg aacgcactat acaaccagat aaccatcgct acataacgca gtggcgtttt
     2281 cggggcgatc gcgccaatca gcgcagcacc cagaacgccg aataatgcgc agcgttcata
     2341 aatacagagc acgcaaggtt tcagtaacat cacatgctgg aaccacagcg ccgtcagttc
     2401 cagtgccaga gcagtaaacg ccatcaacag ccacgcgccc cggccttgtg aacattggtt
     2461 caaaaatcgc aacataatca tttccctgca atatgcatag agtgcgcagt ttaaaccaat
     2521 tcattcgctg cgccaccagg aggcagcaaa aataggacgc aattggacaa ttatcgggcg
     2581 aaaaggcaaa ccgggcattt atatgcccgg taagttgtta tcaaagcgtt gctatccagc
     2641 ccatttgcat aaaccattcg gttacagggg caagcgtaaa ctcgacgcag agcagtccga
     2701 cgagtgtcag tacgagggtg taaggcaggg ccatccacac catgcggcca taagagaggc
     2761 gaatcaatgg cgcgagtgca gaggtcagca ggaacaggaa cgcagcctga ccgttcggcg
     2821 tagcgacgga gggcagattg gtaccggtat taatggcgac cgccagcagc tcgtattgct
     2881 tcaacgtgat agcgccactt tccattgccg ctttcgcttc gttgatataa atcgtcccca
     2941 cgaagacgtt atccgaaatg gatgacagca gaccgttgaa aatatagaac agcgacagct
     3001 gagcatgttc cgatgcctgc aacacaaact gaataattgg cgaaaacagt tgttggtcga
     3061 taatcaccgc gactaccgag aaaaacaccg tcaacagtgc ggtgaatggc agagattcgg
     3121 tgaaggcttt accgatagca tgctcatcgg tgacaccggt caatgatgtt gccagaataa
     3181 tgactgacaa accaatcaag ccaacttccg ccagatgcaa cgccagcgca gtcaccagcc
     3241 agacgccaat aatcgcctgg acaatcagac ggattttatc ctgacgggta cgctggtggc
     3301 ggctttgatc gtcaaactgt tgcaacactt cgcggacttt ctccggcagc gtttcaccgt
     3361 agccaaacca acgcagcttc tctaccagca ggcaggttaa caggccacaa atcagaaccg
     3421 gaacggtcac cggcgacatg cgcaggaaga aatcgccaaa atgccagcca gccgctttag
     3481 cgatgatcag gttctgtggt tcgcccacca tggtcattac gccgcctaat gcggtgccga
     3541 caccggcatg catcatcagg ctacgcagaa agccacggaa ctgttccaga accactttgt
     3601 aatgcttgtc gatatgacta tcgtcttgca ggtcggtgtc ttcggtacgg gaagaggcta
     3661 cgcgatgata aataccataa aaaccgactg caacgctgat caccaccgcc acgacggtta
     3721 aggcatcgag gaacgcggag aggaacgcag ccgccacgca aaaagagagc gacagcagca
     3781 ttttggagcg aatgcttaac agcaaacggg taaatatgaa cagcaacagc tgtttcataa
     3841 aatagatacc cgccaccata aacatcagta acagcaagac ttcaagattt gccgccacct
     3901 cttcacggac gtgttccgcg ctggtcatgc cgatgaatac cgcttcgata gccaacagac
     3961 caccggggag cagcgggtag catttcaggg ccatcgccag agtgaaaata aattccgcta
     4021 ccagcaacca gcccgcgacg aaagggctga tgaggaaaat taacggattt acgattaaga
     4081 aaatgatgag ggcgagtttg taccagtcgg gggactggcc caaaaaattg cgccatagcg
     4141 cgcggcccca ggagatctcc atgatggttt cccttacctt acaaataatc aatgatgttt
     4201 ttatgtttaa acgcaaagct taacggtcag gcaggagtga ggcaagtctt gatggtcaag
     4261 gggaaagaga tgcggaaaat gaagccttga tccctttttt cttctttttg tctgctatca
     4321 gcgtagttag ccctctggta tgatgagtcc aactttgttt tgctgtgtta tggaaatctc
     4381 actatggtca ttaaggcgca aagcccggcg ggtttcgcgg aagagtacat tattgaaagt
     4441 atctggaata accgcttccc tcccgggact attttgcccg cagaacgtga actttcagaa
     4501 ttaattggcg taacgcgtac tacgttacgt gaagtgttac agcgtctggc acgagatggc
     4561 tggttgacca ttcaacatgg caagccgacg aaggtgaata atttctggga aacttccggt
     4621 ttaaatatcc ttgaaacact ggcgcgactg gatcacgaaa gtgtgccgca gcttattgat
     4681 aatttgctgt cggtgcgtac caatatttcc actattttta ttcgcaccgc gtttcgtcag
     4741 catcccgata aagcgcagga agtgctggct accgctaatg aagtggccga tcacgccgat
     4801 gcctttgccg agctggatta caacatattc cgcggcctgg cgtttgcttc cggcaacccg
     4861 atttacggtc tgattcttaa cgggatgaaa gggctgtata cgcgtattgg tcgtcactat
     4921 ttcgccaatc cggaagcgcg cagtctggcg ctgggcttct accacaaact gtcggcgttg
     4981 tgcagtgaag gcgcgcacga tcaggtgtac gaaacagtgc gtcgctatgg gcatgagagt
     5041 ggcgagattt ggcaccggat gcagaaaaat ctgccgggtg atttagccat tcaggggcga
     5101 taatcccttc cgtttaaaga gcaaacccct caaacgaggg gttttttgtt gtttttacag
     5161 atttcccatt cttggcgggc aacgttccag caactcgatg ctgccgtctt cgttttgctg
     5221 ttcgagcatc acatcaaatc cccacaggcg atgcacatgc ttcagcactt ctttgcgccc
     5281 ccgatccagc ggtgcgcgat tatgtggaat gtaacgcagc gtcagcgaac ggtcgccgcg
     5341 caaatccacg ttccagatct gaatattcgg ctccagatta cttaagttat attgcgacga
     5401 taaccggtta cggatctccc gataaccttc ttcattatga atagcggaaa tctccagata
     5461 attatgccga tcatcatcca gcacggtgaa gaagcggaaa tcacgcatca ctttcggtga
     5521 cagaaactgg ctgataaaac tctcatcttt gaaatcacgc atcgcgaaat gcagcgtttc
     5581 cagccagtcg gaaccggcga tatccgggaa ccagtatttg tcttcttccg ttggcgactg
     5641 acaaatccgt ttaatatcct ggaacatggc gaacccgagg gcatatgggt tgatgccgct
     5701 gtaccacggg ctgttatagg ggggctggaa gaccacattg gtgtggctgt gcaaaaactc
     5761 cagcataaaa cgttccgtta ctttcccttc atcatacaga tggttaagga tggtatagtg
     5821 ccagaaggtc gcccagcctt cgttcatcac ctgagtctgt ttttgcggat aaaaatactg
     5881 gctcacctta cgcacaatac gcaggatttc acgctgccat gattccagca gtggcgcatt
     5941 tttctccata aaatagagca ggttttcttg tggttcggac ggatagcggc gcgcttcagc
     6001 aaccgttttc tcttcctcgc gcttcggcag ggtacgccag agcatattga cctgactttg
     6061 tagatactct tcgcgacttt tctgccgggc tttctcttct tgcagcgaga ttttttgcgg
     6121 gcgtttgtac cggtccacgc cgtagttcat cagcgcgtgg cacgagtcca gaagccgttc
     6181 tacttcatca acgccataac gttcttcgca ctcggtaata tatttacggg caaaaatcag
     6241 ataatcgaca atcgaactgg cgtcggtcca actgcggaat aagtaattgt ttttaaagaa
     6301 agagttatgc ccatagcagg cgtgtgccat caccagcgcc tgcatggtaa tggtgttctc
     6361 ttccatcagg taagcgatac acgggttaga gttaatgacg atttcatagg ccagtccttg
     6421 ctgaccgtgc ttatacagcc gttcagtctc gataaacttt ttaccgaatg accagtgcgg
     6481 atagttaatt ggcatgccga cgctggagta ggcatccatc atctgttctg aggttatcac
     6541 ttcaatctgg tgcgggtagg tatccagccg gtagagtttc gccacccggt ctatctctgc
     6601 cagataaaca tccagcaggt cgaacgtcca gtcgggtcca tcgctcaaac gtgtggtgtc
     6661 cttattcata gaatcgatcg tcgccatacg cgcacctcat tgttgtcggc gctctctgtg
     6721 tggagcacct catttcaagc atagaacacc tgttaaaaac cgcgtcgccg gagaattttt
     6781 ttctttgcga tttcttatta tcagagtgcc actaatccgc ttctgaacgg aattttatgc
     6841 tggataaaaa gggcgttcag caggagatac taaagacgcc atattgccgc agagtcaggg
     6901 agatgtgagc cagctcacca taaaaaagcc gtatgttgaa taatattttc aactgagtta
     6961 tcaagatgta attagattat tattctttta ctgtatctac cgttatcgga gtggctatgc
     7021 gagttgtcat actgggaagt ggtgtggtag gcgttgccag cgcctggtac ttaaatcagg
     7081 caggacatga ggtcaccgtc attgatcggg agccgggggc cgccctggaa accagcgccg
     7141 cgaatgccgg gcaaatctcc cccggatatg ctgcaccgtg ggcggcacca ggtgtgcctt
     7201 taaaagcgat taaatggatg ttccagcgcc atgcgccgct ggcggttcgt ctcgacggta
     7261 cgcagttcca gttgaaatgg atgtggcaaa tgttacgtaa ctgcgacacc agccactaca
     7321 tggaaaacaa agggcggatg gtgcgtctgg cggaatacag ccgtgattgc ctgaaagcat
     7381 tgcgcgccga aaccaatatt cagtatgaag ggcgtcaggg tgggacgctg caactgttcc
     7441 gtaccgaaca acagtatgaa aatgcgaccc gcgatatcgc cgtgctggaa gatgccggcg
     7501 taccgtatca gctgctggaa tccagccgcc tggcggaagt ggagcccgcg ctggcagaag
     7561 tagcgcacaa actgacgggt ggtctgcaac tgcccaatga tgaaaccgga gactgccagc
     7621 tgtttaccca gaatctggcg cggatggcag agcaggcggg ggttaaattc cgctttaata
     7681 cgcccgttga ccaactgctt tgcgacggcg agcaaatcta cggcgtgaag tgtggcgatg
     7741 aagtgattaa ggccgatgcg tatgtgatgg cgtttggttc ttactcgacg gcgatgctca
     7801 aaggcattgt tgatattccg gtgtatccgc tgaaaggcta ctcgctgact attccaattg
     7861 cgcaggaaga tggtgcgcca gtatccacca ttcttgatga aacctacaaa atcgccatta
     7921 cccgtttcga taaccgcatt cgtgttggcg gaatggcgga aattgttggt tttaacaccg
     7981 agctgttgca accgcgtcgt gaaacgctgg aaatggtggt tcgcgatctc tatccacgcg
     8041 gcggtcatgt cgagcaggcg actttctgga cgggtttgcg cccgatgacg ccagacggta
     8101 cgccggttgt cgggcgtaca cgctttaaaa atctgtggct gaataccggt cacggcacgc
     8161 tcggctggac gatggcttgc ggttccggtc agttgttaag cgatctgctc tctggtcgca
     8221 cgccagcgat cccatatgag gatctaagcg tagcgcgcta cagccgtgga tttacgccat
     8281 cacgtccggg ccatttacat ggcgcacaca gctaaggaaa cgagatgacc cgtccgatac
     8341 aggccagcct cgatctgcag gcattaaaac agaatctgtc cattgtccgc caggccgcgc
     8401 cgcacgcgcg cgtctggtcg gtggtaaaag cgaacgctta cgggcatggt attgagcgta
     8461 tctggagcgc gctcggggcc accgatggct ttgcattact taacctggaa gaggcaataa
     8521 cgttacgtga gcgcggctgg aaggggccga tcctgatgct ggaaggattt ttccatgctc
     8581 aggatctgga gatttatgac cagcaccgcc tgaccacctg cgtacacagc aactggcagc
     8641 tcaaagcact gcaaaatgcg cggctaaaag caccgttgga tatttatctt aaagtgaaca
     8701 gtgggatgaa tcggttgggc ttccagcccg atcgcgtgct taccgtctgg cagcagttgc
     8761 gggcaatggc gaatgttggc gagatgaccc tgatgtcgca ttttgccgaa gcggaacatc
     8821 ctgatggaat ttccagcgcg atggcgcgta ttgagcaggc ggcggaaggg ctggagtgtc
     8881 ggcgttcgtt gtccaattcg gcggcgactc tgtggcaccc ggaagcgcat tttgactggg
     8941 ttcggcctgg cattattttg tatggcgctt cgccgtccgg tcagtggcgt gatatcgcca
     9001 ataccggatt acgtccggtg atgacgctaa gcagtgagat tattggtgtc cagacgctaa
     9061 aagcgggtga gcgtgtgggc tacggcggtc gctatactgc gcgcgatgaa cagcgaatcg
     9121 gcattgtcgc cgcagggtac gccgacggtt atccgcgcca cgcgcctacc ggtacccctg
     9181 ttttagtgga cggcgtgcgc accatgacgg tggggaccgt ctcgatggat atgctagcgg
     9241 tcgatttaac gccttgcccg caggcgggga ttggtacgcc ggttgagctg tggggcaagg
     9301 agatcaaaat tgatgatgtc gccgccgctg ccggaacggt gggctatgag ttgatgtgcg
     9361 cgctggcgtt acgcgtcccg gttgtgacag tgtaacttgt tgtaagccgg atcggagaca
     9421 gcgtcttctg gttgcgaaaa aatcatccat ccggctggtc agcaactgta gttgttaatg
     9481 tgacagagcc attgcccatg atagtgtcca ttaaaagaat ggacactatt tccccggaac
     9541 ctgaactcac cgcacaggcg ttctacataa aacgcttacg cttcattgtt gactcgaact
     9601 cgacttcaga taaatcacgc tttacccttg atggagcctg tacatagatt tgtgtaattg
     9661 cctgattttg atatgttcaa ttcaacatca aatgaaggtt aaattatgga cgacaaacaa
     9721 ttgcaggctc aggctgcgtt cagcaaagca tcgcaaccgg cgatagatgc ttcattaaat
     9781 ttaagattca gcttcctctt cagccacccg tacgccaatc ttcaacactt cattatcttc
     9841 tttctcggcc accgtccaga tcatcccggc aaactctacc tggtcaccga caaccggtgc
     9901 cgcgcctaac aactgctgga caatttcacc cagcgtttgc tgcttatcac gatattctcg
     9961 cccgtcttcc aggccatata tcagcgcaac atcagcatat ttggcgctgg cttcgagaat
    10021 gaagtcacca aagaagcgtt gatccagcgc gaccggcggc gactggctga acagtttacc
    10081 gagcgccgga agatcgcgtt cccgaccaat tacacacaac acatcgcctt cgcgcagtcg
    10141 ggtgctgccg gtaggatgaa gcaactggtt atcacgaaac agtgccgcaa tacgcgtctc
    10201 ttttggcata tgcaaatcgc gcagtgccgc gcccacgcac catttatcgg cactcaattg
    10261 ataaacaaac tgctcccacg gattttccgg atgaatatcc aggccaacgc gtgacaccgg
    10321 acgtcccact ggcggaacca ccactttggc ttttttagcc gcccacgaga gtgatgttcc
    10381 ctgcaagagc agtgaaacca gaaccacaaa gaaggcgaca ttaaagaaca gacgtgcatt
    10441 ctccagcccc gccatcatcg ggaacacggc caggatgatt ggcaccgcgc cgcgtaatcc
    10501 tacccagctg ataaacacgc gctcacgcag attgaaaccg cggaagggga gcaatccagc
    10561 aaataccgaa aggggacggg cgaagaatat catccatgcc gacaaaatga gcgccggaat
    10621 ggcaataggc agcagatcgc ttgggttaac caatagcccc agcaccagga acatggcgat
    10681 ttgcgccagc caggcgaggc cgtcgaaatt ttgcaggatg ccgtagcggt tgcgaatcgg
    10741 ccgattaccc agcagaaaac cgcacagata aacagccaga ataccgctgc cttccagcgc
    10801 cgtggttaaa gcaaaaatta aaataccgcc gcttaatgcc agcaatggat ataatccggc
    10861 aggcagggcg atgcgattaa tcatttgcag cagtaaataa ccaccgccaa gtccgatgac
    10921 aatcccgagg ccaaattgct gcagaatatc gaccacgaac atccaactga cactgctttc
    10981 atgttgctgg atcatcgcaa tcagggtaat cgtcagaaag accgccattg gatcattact
    11041 gccggattca atttccagcg tcgagccaac acgttcgtta agccccttac cacccagcag
    11101 agaaaagacc gctgcagcat cggtagagcc gacgatagcg ccgattaata agccttcaat
    11161 caaatcaaga ttaaacagcc acgccgccat catgccggtt aaaccagagg tgataagcac
    11221 gcccagcgtc gccagcgaca gtgccggtcc taacgccaca cgaaaggaac tggcctgagt
    11281 gcgcatcccg ccgtcgagca aaataatcgc cagtgccagg ttacttacca tgtaggcgaa
    11341 ggggtaatta tcaaacggga taccgccgac gccatcgact cctgccagca tgccgatcgc
    11401 cagaaaaata accagaatag gaatgccaag acgggaagaa aatgaactaa gtaatatact
    11461 gctggtgaca aggatggatc ctaagatgaa aaggctaatt attgttgtgg catccaacgg
    11521 tcggttactc ctgattacgc tgtctcttat ataaacccta ccatattagc ggcagagaca
    11581 gcgttttact tagccctgaa gcgtgatttt tttacatttt aagaacagga tgaccgctaa
    11641 tcgtcaactg agtgccttcc cgggtgttat tcagaatggc atgtgcgccc aacggcagcg
    11701 taaccgtgcg ttgttcatga ccaaaatcga ggccggtaat gagtggaatc gacaggcggg
    11761 aacgcaaaaa cgcgtacact gactccaggt tgtaacctgc gtcataatca ttgggcgtgc
    11821 tgccgctaaa gctaccgaga ataatcgcct tctgacgtgg caaaattccc gcatgataga
    11881 gctgcaacag catacgttcg acgcggaaag ggtgctcgtt aatatcttcc agcaccagaa
    11941 taccgttctc aatttttggc atccacggtg taccaatcag tgaaatcagc atcgcaagat
    12001 tgcctcccca caacgtgcct tcggtctgac atgtcgggcc ttccccttgc cattcaatgg
    12061 tgaaggtttt attgcgtaac gccagccaga aatggtgctc ggtaaaggcg ttcagttcat
    12121 ccgcaccaaa gtttgctacc agcatggggc cgctaaaggt gatgacattg ccttgcgcca
    12181 gaagaccgca ctgaatggcg gtaaaatcgc tatgtccgca aatgagtaac gggtcatgtt
    12241 gttggcgagc caccagtgcc tgccagtcaa tatctgccag taaacgactg gcaccgtaac
    12301 cgccgcgtac agccagcacg atggtgttgg gggtagtcag tctggcaagg gaattgagat
    12361 cttccaggcg ctccgtttcc gtacccgcaa aacgctcaca acgacgggca ataacctcga
    12421 cgttattgac ctgatgcccc tcgtcggtaa ggcgatggat accacgcagc gcggcgtgct
    12481 gtttaatgca gtaacccgat ggggcaatta agtgaaacag agacatggca attccttgct
    12541 gacaacagaa acgaaatgta tatcatgccg cttaggtgtg ccgttgtcac ctcaacggcg
    12601 attccaggct ataaggatag aagaagtgaa attgagatgg tttgcctttt tgattgtgtt
    12661 attagcgggt tgttcatcaa agcatgacta tacgaacccg ccgtggaacg cgaaagttcc
    12721 ggtgcaacgt gcgatgcagt ggatgccaat aagccagaaa gccggtgcag cctggggcgt
    12781 cgatccacaa ttgatcacgg cgattatcgc tatcgaatcg ggtggtaatc cgaacgcggt
    12841 gagtaaatcg aatgccattg gtttgatgca gttaaaagct tcaacctccg gacgtgatgt
    12901 ttatcgccgt atgggctgga gtggtgagcc gacgaccagc gagctgaaaa atccagagcg
    12961 taatatttca atgggggcgg cttacctgaa tattctggaa accggcccgc tggcaggcat
    13021 tgaagatccg aaggtactgc aatatgcgct ggtggtgtca tacgctaacg gggcaggtgc
    13081 gctgctacgg actttctcgt cagatcggaa aaaggcgatc agcaaaatca acgatttgga
    13141 tgctgacgag ttcctcgatc acgtagcgcg aaatcatcct gcgccgcagg ctccgcgcta
    13201 tatctacaaa cttgagcagg cactggacgc gatgtaaatc agtcgcgcac tttgtccgct
    13261 ttttctcggg cttctcgctc gagagagaaa ataatccgct gtaattgccg ctccaccgtc
    13321 gggctgacgt taagaaaacg gaagctcaga cggggagtgg tgatggtttc attcttgcca
    13381 tcaatcactt tgcgctcgct gatggagatt aactgggcgt caaagtgaaa aacaccccat
    13441 tgccccatgt tgacttcaat ctgagcgaag cgcatgcctt cttgtaattc ggcaggcttt
    13501 gctgtttcca gtaatgcgcc catgccgcct aacgacaaat catacaggcg gaaacgtaac
    13561 gtactgttat ccgccagttt ggtctggcaa aaataaggcg gatggagtgg ggcggagatg
    13621 cggaaatatc ggcgtcgttg tacaaaccat aaggtgggag gcggtacggt gataaatgcc
    13681 ggaagctgca agtattcact ctgctgtagt tgttcaacag taaactcgac tttcgcaccc
    13741 tgagtttcgg cggtaatggt aatgtgctgt gcctttagca cggcgatgtt gtcttcggct
    13801 tgactgccga aatccagcac cagtttatcc ggggttattg ccagtatttt gctgatcagc
    13861 tgcccgccat tccaactgag acgcaaagga attgcggctt tgtgcaaatc gcgtaacacg
    13921 cccaggacgg ctaacggatt ttgtttcagg aactgctcat ggtaatgact cacgcggaaa
    13981 aactcctgac tgacaaactg tctttggttt atcggtcaca gttaacaaaa cttaatacaa
    14041 atgtgtgaat attttttaca tgctgttctt aaatcagccg acatacgccc agcatcgatc
    14101 ccgcccttgc ctatacttag agcgttgatg taagcatttc ttgcgtccga ttcatcgaaa
    14161 cgagggcctg aacatgggaa ttattgcctg gattattttt ggcctgatag ccggcattat
    14221 cgccaagcta atcatgccgg ggcgtgatgg tggtggattt ttcctgacct gtattctcgg
    14281 gatagtcggt gcggtggtcg gcggctggct ggcgaccatg tttggcattg gcggctccat
    14341 cagtggtttt aatctgcaca gcttcctggt ggcggtggtg ggagctattc tcgttctggg
    14401 catattccgc ctcctgcgaa gagaataaga ttttcataag gcggatagcg atacagatgc
    14461 cgctatccgc tttcacatca gaacgtatac tcgacacctg ctttacgggt ggaacaaatc
    14521 aatatagcac ttagatgccc gataaagtcg gttcccggta acaacaacag aatccttttt
    14581 taattattgt ttcgttgttt ttggtgtgat tcagaaaata aatattttag acattagcca
    14641 gtcaggcgtt aaaaatgggg cggatagtga ggcggttttg gctgcgctta ttgaacattt
    14701 agaattgatc aatccttctg gcagattaag ccaaaatacc cgttcggcaa tgcttcagtt
    14761 acgcgaagag gaatggtctg aattcttttt ttggttgcta aattctctgg agtgtctgga
    14821 ttatgtgatt attaatttaa ctccagaaag caaaaaaacg ttgatgagtg aacaccgtaa
    14881 caatattcag gtagcaatcg atgctttata taggcaacgt cggtgtaaat caccgggtga
    14941 tgaaagtgaa acacttaccc gtagaaatga cgctatcttc ggcaaccacg tatggcaaac
    15001 atttgcgcaa tacttcccgc caggacttga aaaaccgtct gtgtagaaaa caccatagct
    15061 atggtgttat ctgccgacaa cgccatccgg agaagccgaa tgacgttgtc ggagtaagcg
    15121 ctggttaagg tgtgggttgt gcctctttgg ttgagggttg cgtcgttgct gacttaacgg
    15181 tcggacgcgt cgccggaaca ttgtcacacg gttgctcttt cgggcagatc aaatccagca
    15241 ttttcagcgt cacgccattg gtccagccaa agccatcctg taatggatat tcgccaccgc
    15301 cgccccccgt tccggtggtg ctgacatcat atttttccac cagctttttc tcccggtcat
    15361 aggtgtgctg aacattggtc aggaagtgcc agctaatgtc catcgccacc tctttttgcc
    15421 cgtagttttg taatccttct gtcgcgaccc actgtaacgg tgcccagcca tttggcgcat
    15481 cccattgttg cccacttttc accgacgtgg tgttcaggcc gccgggttgc agcagatgtg
    15541 ttttcgtcgc cgtcgccatt ttgttggcgc gatctttcgc tgccgcattg acgtacagcg
    15601 ggaacagggc ggccgcggtt aactgattgc gcactttata acttttcagg tcgtaatcgg
    15661 cataccagcc ttgttgatcg ttccacatgt atttttcgat ccctttttga cgggcatttg
    15721 ccagcgtttc gtactggttt gccatcgcgt tatctccggc agctttgctg gcgcgggcga
    15781 ggattttttc cattttaaac atcaggctgt tcagatcgac cggtacgatg ctggtggtgc
    15841 gtaaggtatt tagttgatgc gggttgtcca tccagcgcga gctgaaatcc cagccagacg
    15901 cagcggcaga gcgcaggtcg cggtaaattt cagtggcagg tcgattcgga ttgcttttgg
    15961 cggtggcaat atcttccacc catgactctg gtcgtggcgt atcgcgatcg tcccagtagc
    16021 ggttgagaag ggtaccatcc tgaagtttta caacgcgttt ttcctgttgt ccggcttgca
    16081 ggttttcaac accgtccatc cagtaagcat attctttttg catttgcggc aggtattgct
    16141 tcaacgcggc atcgccttca tgctgcgcca gtaactctac catcagggca aagaagggcg
    16201 gttgcgagcg gcttaaatag taactgcggt tgccgttggg aatatgaccg taagtgtcta
    16261 tttcatgagc aaaattggcc accatatccg cgactttatc ccagtgaccg ctttcggcaa
    16321 gtcctaacat ggtgaagtaa ctgtcccagt aatatacctc gcgaaaacgt ccgcccggca
    16381 cgacataagg ttccggcagc ggtaacagag aatcccattt ttcggtgttt tcggtagaac
    16441 gcgttaatac cggccaaagt ccgtcaatat gttcgcgcag tgactgcccc tctggcggaa
    16501 catatttctc gccttctttc ggcagggtga aattgacgtt aacgaaatgg cgcagatcaa
    16561 atccgctctg gttttgctgc atccgataat cagcaaggat catcagcgga tcgctgttcg
    16621 gcacggcatc ggcaaaggtt ttttggtccg gaaaaagttt ggcgttttgc acatcattaa
    16681 acagcggccc taataaaata tcaggcggct gtggtgttac cggtgtttct tctgcctgca
    16741 ccgatagcgc agcgaaacac aaaaagatac aggctggaat taacgccatt ttttgcgggc
    16801 gagaaggtgc gggggatttc atcaatcatt ctccttaggc gaaaccgaat aaaacggtat
    16861 tcaagtatca gaaaacctta gttcaggatc gcactagctg catgatcgaa atctcattct
    16921 gcgaactgtc aggcatttta gaaaagggaa ttaaagggat aaataacggc gaaatagagt
    16981 gatcaaataa cgtctgttta ttagccagcc agcgacattt ggttcgcgat tagcgaagaa
    17041 aaatcatcat aaattggtga aaaatataac aggtagttaa aaaacattag tgctgagtaa
    17101 attgccggat gacatcagaa cgatgccatc cgaacggtgg cttaaccctg acggttgaaa
    17161 cgttgcgttt taacgtccag cgttagcttt tcttctggtt gtatcgcata cagtttctca
    17221 ccctgctggc aaatccagcc aatctccagt tcacgggcga ttagggcgct gtgggatacc
    17281 ggacttccgg cgctaaggca gatacctttt acaaccgccg gatccagttg cagtactgtg
    17341 gaaggataaa tgttctccgc cagtagaata gttggcgagt taaactgcgg gagttcttct
    17401 ttcgtttggg tcaggtggac cagggtgcga tgcagaagat cgtccacatc aatatagcga
    17461 gcttgtagat attcatcatc cagttgctgg tattgctggc taagttcttt aagaacttgc
    17521 tgccaggcat attctgccgt gcaatgttcg tgctgaagga gttcgcttgc cgccgccagc
    17581 agttccggat catctaacag tgtatggtga ccagaaaaga ttgcggcaat atcgtcaagc
    17641 ccgctggctt ctgctttcgc tgttaacgtc atcagatcta ataacgtgaa gtcaatggcc
    17701 tggcgtaatc gatcttgttc ctcttccacg gtcagggttg atttggcctg taccgtacat
    17761 aaaaccggtt gataataaaa tgctttaccc gaaacaggcg gaacgggacg cagagtaggt
    17821 ggagcaactt cctccgtttc accaaagtta tcttcagcca gctgacggaa agcgatcagt
    17881 gcctcttcag cttctggccc tttcgcaatc aggcgcagcg tatcgttata gcgaacttgt
    17941 agtaacgcaa tctggttaat actctccggt gtgacgcatt tgccgttttt ctccagcaac
    18001 atatcggcat taaatgtcga taaggtataa accagccggg aggctggacg tacatgcagg
    18061 ccgttacggt tctttatgac caccgccaga gaacgggctt cttcatcgta cgcaggacat
    18121 gtgtcagaga tttcagtatc ggaggacagt aaacccagtt gttcacgttt ggcttccagc
    18181 gcatgcatgg cgtcaaagat aactttgtcg atatccgccc ccgaggccgc gctgaccgtt
    18241 gctgccagtg taccttcgac caacggcgca gcacacaaac gtacttttgc ggcgatctcg
    18301 ggagccagca attccagcgc agtttcagca ctcaataatg cgctacccat atccatcatg
    18361 accagcacat ggtcggcatc ggcaacagat tcgatggcct ccatcacttt gacggcatcg
    18421 gtaccaatgg gattttgtgg atcgtcaatt cccgcggcaa tggcgatttt acaactatca
    18481 ctcattaaca tctgacgggc taattcaccg acaccttctc ccagtcggct gctatgtgaa
    18541 actatgacca ggtttaccat caccaattcc ttactctttt gcggctaacg ccaacatttg
    18601 catcataaac atcaccgagg ttgcgccggg atcctggtga ccaatactgc gttcaccgag
    18661 ataactggcg cggcctttgc gggcttgcat tgtaatcgta ctttgtgcag cggattcggc
    18721 gatgctactg gctgcttcga gcgccgccgg aacagagaga ttttgctcgc tggactgacg
    18781 taacgattcc accaccggca cccacacatc acacatggtt ttatcgccag gttcggcttt
    18841 cccgcgactg attacgccgt ccgcgccatc gcggaacatc tgataaagct cttccagcgt
    18901 caggctttgc cgtgcctggg tcgcctgtgc tgcgcgtata aagaaagtac cgaacagcgg
    18961 accactggca ccaccgacgc tggaaagcag cgtcataccg gtattcttga gaatgaaacc
    19021 gatatcttta tctgcgatag cagggagttt ttccaccact ttgctaaagc ctcggttcat
    19081 atttagcccg tggtcagcgt cgccaatttc gcgatccagt ccggtaagat acccgctctc
    19141 ggtgttgaaa atatcgccac aacgagtgag ccagttaaca atttgagttc tgctcagtga
    19201 cattgctttc tccttattta ccccagttaa gggccggggt gtggaccggg gcgtcccaga
    19261 gagccagcgt ttcgtcatca actttcagta aggtgattga gaaaccggtc atatccagtg
    19321 aggtgcaata cgcgccaatt aaattacgct cgatagtcaa tcccgcttgc tggcaacgtg
    19381 tggtcaggcg gttatagacg ccgtacagct cagaaagcgg agttgcgcca agattgttaa
    19441 ccagcgcaat cacccgatcg ccagactgga gctgttgttt ggtttgtggt tcttcctgcc
    19501 aactgccttg ttgataatcc cagaaacgca aagtgcgatg gtatgagcca ttttccagca
    19561 gggtgtcgaa catttcatcg acggtttgat caagggaaga gaaggggcgg cggtcaatac
    19621 ccggctcacc atgaatgccg acgccaaact ccatctcatt gtccgccagg gtaaaagaag
    19681 gtttgcccgc ggcaggaacg gtacaggcac cgagagcgat acctattgag tggccttgat
    19741 tattcaactt acgccccagt tccgcacagg cgtccagtga gtcgccacgc tccgccgctg
    19801 cgcctacgag tttttcaatt aatacggtgt tggcaacgcc gcgtcgcccg gcggtataaa
    19861 gactgtcttt tactgcaacg tcgtcatcaa tgaccacagt ggtcactttt acgccgctat
    19921 cgtgcagtaa ctcggttgct gtttcgaagt taagaatatc gccggtgtaa tttttgataa
    19981 tcaacagtac accttcgccg ccatcaactt gcatggcgca ttcaaagatt ttatcgggcg
    20041 tcggtgaggt gaaaatttcg cccggacagg cccccgaaag catcccctga ccgatataac
    20101 cacagtgcat cggctcgtgt ccgctgccgc cacccgacag cagggcgact tttcctgcaa
    20161 cgggggcatc agctcgggtg acatacaccg gttcctgatg cagtgtcagc gatggatgcg
    20221 ctttcgccag tcctgccagt tgttcgtcca gtacgtcttg cacatcattg atcaattttt
    20281 tcattatttt gctccagcaa ttacggtagg gcatggatga tgttcaacga cacggcgacc
    20341 actgactgcc gatgaatcca ttgtgcatca ggcaagggaa aagaaaatta gcccaaatta
    20401 tgtttcatag tgaaacatat gctttaatga atgttccata ttgaaacttt tacgtgtatt
    20461 aat
//
LOCUS       DAJOWB010000083        20381 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJOWB010000083 DAJOWB010000000
VERSION     DAJOWB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.083"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(110..856)
                     /gene="cutC"
                     /locus_tag="OHO85_003221"
     CDS             complement(110..856)
                     /gene="cutC"
                     /locus_tag="OHO85_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCQ2672564.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(870..1436)
                     /locus_tag="OHO85_003222"
     CDS             complement(870..1436)
                     /locus_tag="OHO85_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCQ2672565.1"
                     /translation="MANWQSIDELQDIASDLPRFIHALDELSRRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1652..3385
                     /gene="argS"
                     /locus_tag="OHO85_003223"
     CDS             1652..3385
                     /gene="argS"
                     /locus_tag="OHO85_003223"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCQ2672566.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            3562..4050
                     /locus_tag="OHO85_003224"
     CDS             3562..4050
                     /locus_tag="OHO85_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCQ2672567.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DVNVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4170..4562)
                     /gene="flhe"
                     /locus_tag="OHO85_003225"
     CDS             complement(4170..4562)
                     /gene="flhe"
                     /locus_tag="OHO85_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCQ2672568.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4562..6640)
                     /gene="flhA"
                     /locus_tag="OHO85_003226"
     CDS             complement(4562..6640)
                     /gene="flhA"
                     /locus_tag="OHO85_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HCQ2672569.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NIVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6633..7781)
                     /gene="flhB"
                     /locus_tag="OHO85_003227"
     CDS             complement(6633..7781)
                     /gene="flhB"
                     /locus_tag="OHO85_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HCQ2672570.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7983..8627)
                     /gene="cheZ"
                     /locus_tag="OHO85_003228"
     CDS             complement(7983..8627)
                     /gene="cheZ"
                     /locus_tag="OHO85_003228"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCQ2672571.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8638..9027)
                     /gene="cheY"
                     /locus_tag="OHO85_003229"
     CDS             complement(8638..9027)
                     /gene="cheY"
                     /locus_tag="OHO85_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCQ2672572.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9042..10091)
                     /gene="cheB"
                     /locus_tag="OHO85_003230"
     CDS             complement(9042..10091)
                     /gene="cheB"
                     /locus_tag="OHO85_003230"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCQ2672573.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYNEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10094..10954)
                     /gene="cheR"
                     /locus_tag="OHO85_003231"
     CDS             complement(10094..10954)
                     /gene="cheR"
                     /locus_tag="OHO85_003231"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCQ2672574.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10973..12574)
                     /gene="tap"
                     /locus_tag="OHO85_003232"
     CDS             complement(10973..12574)
                     /gene="tap"
                     /locus_tag="OHO85_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCQ2672575.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRGLQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12620..14281)
                     /gene="tar"
                     /locus_tag="OHO85_003233"
     CDS             complement(12620..14281)
                     /gene="tar"
                     /locus_tag="OHO85_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCQ2672576.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14426..14929)
                     /gene="cheW"
                     /locus_tag="OHO85_003234"
     CDS             complement(14426..14929)
                     /gene="cheW"
                     /locus_tag="OHO85_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCQ2672577.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(14950..16914)
                     /gene="cheA"
                     /locus_tag="OHO85_003235"
     CDS             complement(14950..16914)
                     /gene="cheA"
                     /locus_tag="OHO85_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCQ2672578.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILYAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(16919..17845)
                     /gene="motB"
                     /locus_tag="OHO85_003236"
     CDS             complement(16919..17845)
                     /gene="motB"
                     /locus_tag="OHO85_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCQ2672579.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17842..18729)
                     /gene="motA"
                     /locus_tag="OHO85_003237"
     CDS             complement(17842..18729)
                     /gene="motA"
                     /locus_tag="OHO85_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCQ2672580.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLATVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18856..19434)
                     /gene="flhC"
                     /locus_tag="OHO85_003238"
     CDS             complement(18856..19434)
                     /gene="flhC"
                     /locus_tag="OHO85_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCQ2672581.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19437..19787)
                     /gene="flhD"
                     /locus_tag="OHO85_003239"
     CDS             complement(19437..19787)
                     /gene="flhD"
                     /locus_tag="OHO85_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCQ2672582.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4899 a         5592 c         4958 g         4932 t
ORIGIN      
        1 tatcgttcgt tttttatact gttcagggat aaaaaaaggc ccctgttgaa attgcagggg
       61 cctggtacga gcaagcatca tattgggcga catgatgcaa cggtaaaaat catttggcct
      121 gatggcgttc aatgattcct ttcatttcag caaccgccgc cccgtctacg atataacgcg
      181 aatactcgtc cgcgtgttca tctgatgaca tggacaatcc ttgattacga taacgcatcg
      241 gtgaggcttg ccacgctccc gcggagctat ggacttccag cactccggca tcgaggaagt
      301 ggtgcaggtt ttctgcacgg actcctgctc cggccataat gattggagca tcacgatggg
      361 caataagttc cataattttt gataaacctt gcagtgcgtc tgatttttgc cctgatgtca
      421 gtacccgggc aatgcccaat tccgcaagat tattgagtgt atataaaggg ttagcgcaca
      481 tatcgaaggc gcgatgaaaa gtcactgcca gcggaccggc agcagccatt attttttcca
      541 ttcgtggcat atcgacattc ccgtcaacat cgagaacgcc cgtcaccagt ccaggaaaac
      601 ctaattcgcg gaccgtgcgc acatcctcaa gaatggcggc aaactcaccg tcgctgtagc
      661 aaaaatcacc accgcgtggg cgaattatcg gatgcacagg gatcgtcacc cgctggcgca
      721 cggatttcag tacacccaac gacggcgtta agcccccctc ttttggggct gcgcataatt
      781 caactctgtc tgcgccgttt tgctgcgccg ttagtgcaca ttccatgctg taacagcaaa
      841 tttccagtaa tgccattttt actccttaat tacgccgact gctcgctggc aacgatctct
      901 tcaatggacc acggatgaaa cttaatagtt gttttgccat cggtgaccgc cagcgtcgga
      961 ttcggtaatc gctcatgctc accttttggc gaactggttt tcactgagat ccccggtagg
     1021 ctgagtcctt catcagaaag caatgccagc gcacgggcat tcagcgtttc tggatcaccc
     1081 ggcagaacga tttcaatatg ttcccaacct tcgtgtgggt aacgtttttc cccgggccac
     1141 ggtagctcca caatagaaaa ctgccagtgc gcaacctgta ccggttcatg caatttaaac
     1201 agacaaatcg gtctgccatt gatcatattt tctgacaaaa gctcgccaca ctgttcaaac
     1261 ccgcgacgcc agcgttcagc agtggcgttt tgatggcaac gcaaagaaat gtgatcggca
     1321 gtcagcggag tgatattcaa ccccagacgg cgggaaagtt catctaatgc gtggataaat
     1381 cgcggtaaat ccgatgcaat atcctgcagc tcgtcgatag attgccagtt cgccataatc
     1441 actcttcgtc tttcagtaaa agcgttaatt taccctgttg ccctgtgcca accaaccgct
     1501 gatttcacgc cgcttctgat gcaatagtga aaacggcaat acgccacgcg cacgttgctg
     1561 acgaaaacag ccatttgcag tatactcccg ccctaatttc tttaactggt gcgggcaatt
     1621 tttgctcgct tcatcaatgt aaggtattcc ggtgaatatt caggctcttc tctcagaaaa
     1681 agtccgtcag gccatgattg cggcaggcgc gcctgcggat tgcgaaccgc aggttcgtca
     1741 gtcagcaaaa gttcagttcg gcgactatca ggctaacggc atgatggcag ttgctaaaaa
     1801 actgggtatg gcaccgcgac aattagcaga gcaggtgctg actcatctgg atcttaacgg
     1861 tatcgccagc aaagttgaga tcgccggtcc aggctttatc aacattttcc ttgatccggc
     1921 attcctggct gaacatgttc agcaggcgct ggcgtccgat cgtctcggtg ttgctacgcc
     1981 agaaaaacag accattgtgg ttgactactc tgcgccaaac gtggcgaaag agatgcatgt
     2041 cggtcacctg cgctctacca ttattggtga cgcagcagtg cgtactctgg agttcctcgg
     2101 tcacaaagtg attcgcgcaa accacgtcgg cgactggggc actcagttcg gtatgctgat
     2161 tgcatggctg gaaaagcagc agcaggaaaa cgccggtgaa atggagctgg ctgaccttga
     2221 aggtttctac cgcgatgcga aaaagcatta cgatgaagat gaagagttcg ccgagcgcgc
     2281 acgtaactac gtggtaaaac tgcaaagcgg tgacgaatat ttccgcgaga tgtggcgcaa
     2341 actggtcgac atcaccatga cgcagaacca gatcacctac gatcgtctca acgtgacgct
     2401 gacccgtgat gacgtgatgg gcgaaagcct ctacaacccg atgctgccag gaattgtggc
     2461 ggatctcaaa gccaaaggtc tggcagtaga aagcgaaggg gcgaccgtcg tattccttga
     2521 tgagtttaaa aacaaggaag gcgaaccgat gggcgtgatc attcagaaga aagatggcgg
     2581 ctatctctac accaccactg atatcgcctg tgcgaaatat cgttatgaaa cactgcatgc
     2641 cgatcgcgtg ctgtattaca tcgactcccg tcagcatcaa cacctgatgc aggcatgggc
     2701 gatcgtccgt aaagcaggct atgtaccgga atccgtaccg ctggaacacc acatgttcgg
     2761 catgatgctg ggtaaagacg gcaaaccgtt caaaacccgc gcgggtggta cagtgaaact
     2821 ggccgatctg ctggatgaag ccctggaacg tgcacgccgt ctggtggcag aaaagaaccc
     2881 ggatatgcca gccgacgagc tggaaaaact ggctaacgcg gttggtattg gtgcggtgaa
     2941 atatgcggat ctctccaaaa accgcaccac ggactacatc ttcgactggg acaacatgct
     3001 ggcgtttgag ggtaataccg cgccatacat gcagtatgca tacacgcgtg tattgtccgt
     3061 gttccgtaaa gcagaaattg acgaagagca actggctgca gctccggtta tcatccgtga
     3121 agatcgtgaa gcgcaactgg cagctcgcct gctgcagttt gaagaaaccc tcaccgtggt
     3181 tgcccgtgaa ggcacgccgc atgtaatgtg tgcttacctg tacgatctgg ccggtctgtt
     3241 ctctggcttc tacgagcact gcccgatcct cagcgcagaa aacgaagaag tgcgtaacag
     3301 ccgtctaaaa ctggcacaac tgacggcgaa gacgctgaag ctgggtctgg atacgctggg
     3361 tattgagact gtagagcgta tgtaatcgat ttttcgtgag agtgaagcct gatcagggtt
     3421 agccgatcag gcttttttat tgccatctaa atgtattctg aaaatggaca tgccattgtt
     3481 ttctcactgt tggataagag gccagaagcg taatatccgg ccccagggaa acgataacgg
     3541 ttgaatttaa ggaataccgc agtgtttaaa tttcttgtat taacattagg cattatctct
     3601 tgccaggctt acgcagaaga tacggttata gtaaacgacc atgacatttc agccatcaaa
     3661 gattgttggc aaaaaaattc agatgatgat actgacgtta acgtgatcaa atcatgcctg
     3721 cgacaagaat acaatctcgt cgatgcgcaa ttaaataaag cctatggtga agcttatcgt
     3781 tatatagaac aagtgccacg cacaggtgta aaaaaacctg ataccgaaca acttaacttg
     3841 cttaaaaaat cacagcgagc ctggctggat tttagggaca aagaatgtga attaatcctt
     3901 tcaaatgagg acgttcagga tttaagtgac ccttattctg aatcagaatg gctctcatgt
     3961 atgatcatac agaccaatac gcgaactcgc cagttgcagc tataccgtaa ctctgaagat
     4021 ttttatccaa gccctttgac aagaggataa ttcacatctt tttggcatgt tttgttgcaa
     4081 gctattcctg ataaataatt gcaacaagac atcgagcctt tttcactgag ttattaaaca
     4141 tactcgcgag cgcgtaattt ttttgtcctt cagcgataat tcacaatcac ttcattacgt
     4201 tgtaccttta gcggtggaat taaccgccca ccgcctggca cttcccagat aaatcttaac
     4261 ggttctgctg ccgctatacc ggaaaaggcc acggtggttc cgctctgccc ctctaattcg
     4321 acacaacgag attgcgaaca caagcgaacc cgcagtcctg aaggtgtcgg gccgataagc
     4381 tgataacgcc acgctaccag cgtcatcaat cctgaagcag gttgtcgcgt agaaagaggc
     4441 gcagacgaca tcgactcacc gcgatgattt agcgtaattc ctacactact tgcctgccac
     4501 atcccctccc cggcggcttg caccagcagc ggaaacaata atattgctaa taaggttctc
     4561 attatttgcc gccaattgtc gccgtcatgc ggatatgtcg gttatcagac agttccagat
     4621 tcgacaggac cactaactgc ggcaagctgc ggcgcaggaa gcgagacaat aatggtcgca
     4681 gcgcgtggtt caccaacaat actggcggcg cacccagcat ctcctgacgg gatagcgctt
     4741 cctgagtttg cgccagtaaa cgatccgcca gccctggctc cagtcctccc ccgccctgca
     4801 gcgcctgtag taacaaacgt tccagcggtg tatcgaggcc aataacatgg acttcatctt
     4861 tgccaggaaa ccactgctgg gtaatcgccc gtcccaacgc cacgcgcacg acggcggtta
     4921 attcatgtgg atcgctttgg atgggcgcat gttccgccag cgtttcgaga atggtgcgca
     4981 tatcgcgaat cggtactttt tcatcgagga gattttgcag cactttatgc agtgtggtga
     5041 gcgtgacgac gccaggaacg agatcttccg tcagctttgg catctcctgg gcgacgcgat
     5101 ccaacagctg ttgcgcctcc tgacgaccaa acagctctgc ggcatgctgg ctaatgaggt
     5161 ggttaagatg cgttgctacc accgtgctgg cctcaaccac tgtgtacccc tgaatctgcg
     5221 cctgttcttt tagcgcactt tcaatccaga tagcattcag gccaaatgcc ggatcgacgg
     5281 tcgcctcacc aggtaacgtc ccggcagcgg ttccagggtt aatcgccagc cagcgccccg
     5341 gataagcatc accactgcca atctccacgc ctttcatcaa aatgcgatag cgggcaggtt
     5401 gcagatccat attgtcgcga atgtgcacca ctggcggcag aaatcccatc tcctgggcaa
     5461 atttcttgcg gatactgcgt atacggccca acaactcacc atcctgctgg aaatcgacca
     5521 tcgggatcag tcgataaccc acttccattc ccagagaatc ttccagttgt acatcgttcc
     5581 acgtcgcttc gacaacggta ttattctctg ccatttttac cggtttgggt tcggcaggcg
     5641 ctttttgttc gcgtccgcgt atccaccagg ccagcccgag caatccggca gtgaacagca
     5701 aaaataccag gttcggcatt ccaggcacca ggccgagtaa accgagcacg gcggcgctta
     5761 acaacataac gcttgggtta ctgaaaagct gattcaccat ctgctcgcca acatcctgat
     5821 cggtgctgac acgcgtaacg atgacccccg cggcggtaga aatcaccagc gccggaattt
     5881 gtgccaccag accgtcgcca atggtcaata gcgtataact ttccgccgcg tgtcccatgc
     5941 tcatgccatg ttgcagcacg ccgaccagca acccgccgac aatgttaatg accatgatga
     6001 ggatcccggc gatggcatcg ccgcgaacaa acttacttgc cccgtccatt gagccgtaaa
     6061 aatcggcttc ctgagtcact tcggagcggc gttttttcgc ctcatcttca ccaatcaatc
     6121 cggcgttaag gtcggcgtca atcgccatct gcttacccgg cataccatcg agaacaaagc
     6181 gcgcacccac ttctgcgata cgcccggcac ctttggtaat gaccataaag ttgatgatca
     6241 cgagaatgac aaacaccacg ataccgatag cgaaattgcc accaacgagg aagtgaccga
     6301 acgcttcgac caccttccct gccgccgccg cgccggtatg cccttccatt aaaatgatac
     6361 gggttgaagc cacgttaagt gccagacgca acagcgtggt aaacaacaga atggtcggaa
     6421 acgcagcaaa ctcaagcgtg cgctgggtaa acatcgccac cagcaacacc atgatcgaca
     6481 aggcaatatt gaaggtaaac aacaggtcga gtatgaatgc gggcagtggc agcaccatca
     6541 tcgacaagat caacaggatc aaaatcggtc cggcaaggat ctgccattgt gtcgatttca
     6601 ggtttgcggg caggcgcagc atcgcggcca gattactcat gggtcggttt ctcgttaata
     6661 aaatccaggg cttccggcac cggaagatga gtaggttgta cagggcgctg tccaccagcc
     6721 agacgccagc gtttcagttg ccagacccag gccagcactt ccgccaccgc ggcgtacagt
     6781 tgacccggga tttgttgacc aatctccgca tgtcgataca gcgctcgcgc cagcggcggc
     6841 gcttcaagcg tcgggacgtt attttcagcg ccaatttcac gaatgcgcag cgcgaccagc
     6901 cctgcacctt tagcgaccac tttcggtgcg ctcattttgt tttcgtcata ctgcaacgct
     6961 accgaatagt gggtcgggtt attgacaatg acatccgctt tcggcacatc ggccatcatc
     7021 cgacgccgtg cagcagctcg ctgcatctga cggatccgcc ctttaacatg agggtcacct
     7081 tcgctttgtt tgaactcatc acgaatatcc tgccgtgaca tacgcagctt tttcaggtgg
     7141 ctgaatattt ggaaaaagac gtcaaatccc accattggaa tgacaccaag caccaccagc
     7201 agtgcgcata gccctaccaa atccatcgca ttacccatgg cggtaatcgg agactcggcc
     7261 atcaagcgca tcatctgcgg ccagtgatgc cagagaaaaa accccgtcac gctgccaacc
     7321 aggatggttt tcaaaattgc tttaagcaac tccgcgccag tctgagccga gaacatccgt
     7381 ttaatgcccg gtagcgggtt gagtttggaa aacttcggct gcaaggattt gccgctaaat
     7441 accagccctc ccagcatgac cggagaaatg agcgccacca gcaccacgcc gctaatcagc
     7501 ggcagcagcg ccagcatggc ttctctgatc agcagaataa tctgcccgag gatcagattc
     7561 gggtcattga taatactgtg atcaaaatgc agcccagcgg agagcatgcc cgacaatcga
     7621 cgggccagcg acacaccgcc aaaccagata acactaacgc ccaccagcaa aatcagcagt
     7681 gaggtcagtt cacgggaacg cgggatttgc ccctcttccc gcgctttttc tagtcggtga
     7741 ggtgtggggg cttctgtttt gtcgtcgctc tcgtcagaca cgtcgccaat cctggaaaga
     7801 gtattaagcg tgaatgatgc cagagcgcaa agcgttcaat ggtttgagta aggggcaaaa
     7861 caggcgggat ttagggcttt tgctgccaca catcaagcat agtgtgcgtt tgtcggatgc
     7921 ggcgtcatcg ccttatcaga ccgcctgata tgacgtggtc acgccacatc aggcaataca
     7981 aatcaaaatc caagactatc caacaaatcg tccacctgat cctgactggc taccacaccg
     8041 gctttgctgg tatcgacctg aggtccatta agcaaactct ggttttcacg ttttggacgc
     8101 gactcctgtt ccgggatgtt ttccaacagc accatcagca actggcgttc gatctcctga
     8161 atgacatcca tcatccgctt aatgacctgc ccggtgagat cctgaaaatc ctgcgccatc
     8221 atgatttcca gcagttgcgc gttagtaaag ctggtatgcg cgggtacatc tgccagaaat
     8281 tgtcgtgtat ctgttaccag ttcacgggcg tcggcaaggt caatcggatc ggcaaaccag
     8341 tcatcccaac gttgggttaa cgcttttgct gatttctcca tttgatcctg atgcggttgt
     8401 gacgcctcaa cactgttcag cgcccgctcc gcagcctggg cggtcatctg cacaacatag
     8461 tacaaacgat cgcgcgcatc ggggatggct tccgccgctt cggcaatggc ctgatccagc
     8521 cccagttccc gcaaactgtc gcgcagcata cgcgtcaggc tgccgatgcg cgcaatgata
     8581 tcgccagctg aatgctcgtc agcaggtttg attgatggtt gcatcatagt cgcatcctca
     8641 catgcccagt ttctcaaaga ttttgttgag tttttcctcc agcgtcgcgg cggtaaatgg
     8701 cttcaccaca tagccactgg cccccgcttg cgccgcagca atgatgttct ctttcttcgc
     8761 ttctgcagtc accattaaca ctggcaatgc cgacatcgcg ccatccgcac gaattgtttt
     8821 cagcaattcc aggccatcca tattgggcat gttccagtcg gagataacaa atccataacc
     8881 gcctgcctgc aacttattga gagcgtcgac gccatcttcc gcttcctcaa cattattgaa
     8941 tcccagctct ttcagcaggt tacgcactat gcgtcgcatg gtggaaaagt catccacaac
     9001 caaaaattta agttctttat ccgccatttc acactcctga tttaaatacg tatcgcctgt
     9061 ccggcactaa tttttgccaa catttgctgg cttacctggc taagatcgac cacttcgcag
     9121 acaccaccca tattgatggc ctcgcgcggc atgccgaaca ccacgcaact tgcttcgttt
     9181 tgcgcaaggg tccatgcccc cgcctgacgc atcgccaaca ttcccgccgc gccgtcgttg
     9241 cccataccgg tcaggatcac cccaaccgca ttacgccccg cctgtttggc gacagaatgg
     9301 aacaacacat ctaccgaagg ccgatgacgg ttaaccgccg ggccatcgtg aattttgatt
     9361 tggtaatttg cgccactacg cgacagctcc atatgccgat cgcccggcgc aatataagca
     9421 tgccccggca agacacgttc tccgtcttcg gcttctttaa ccccgatctg gcaaagctta
     9481 ttaagtctgt cggcaaaaga gcgggtgaaa ccgggcggca tatgctgggt aattaacagt
     9541 gccgggctgg aaagcggcaa cggttgcagt acgtgacgaa ttgcctcagt tccacccgtt
     9601 gaagcaccaa tcgcaatcag tttttcagaa ctcaacaacg gccccgcctt cagcgttgtc
     9661 ggtgccgaca atggcttatg tgctgcaagg ctcgcctttg ctgccgtacg caccttttca
     9721 gcaatcattt cgttatacgc cagcatacct tcgcgaatac ccagttgcgg tttggtgaca
     9781 aaatctatcg cccccagctc cagcgcgcgc agcgtgactt ctgacccttt gccggtcagg
     9841 gaagaaacca tcacaacggg cattggacgc aaacgcatta atttttcgag gaaatccagt
     9901 ccgtccatcc gcggcatttc aacatccagc gtcagcacat cgggattgaa tttcttaatc
     9961 aagtcacgcg cgaccagcgg atcaggcgcg gtcgccacca tttccatgtc gctatggctg
    10021 ttgatgattt ctgtcatgat ctggcgcatc agtgccgaat catcgacaga taacaccctg
    10081 attttgctca tcgttaatcc ttacttagcg catacaccgt ctgaccacgc agcgtgaagc
    10141 ggcgctcaag gtggctaaag ttttcagagt gacccgcaaa cagcaatccg tcgggtttaa
    10201 ggagcggaac aaagcggcgc aaaatctcct gctgggtatt ttgatcgaag tagatcatga
    10261 cgttacgaca gaagatcgca tcaaacggcc ccggcacggt gtactgtttc gccagtagat
    10321 tcagcggggc aaaatcaaca tagttcgcca gctcctgacg cacgcgtacc agcccttcat
    10381 gcggtcccgt ccctcgcatg aaataccgtt gcagttgctg cggcgtcagg tttttcaact
    10441 cttcatggcg ataaataccg cttctggctt tttccagcac ttcggtgtcg atatcactgg
    10501 caaacacttt ccagcgtccg ggcgcggtgc ccaatgtgtc agccagcgtc atcgcaatgc
    10561 tgtacggctc ttcgccggtc gaagccgccg cgctccatac gcgatactcg ccagaacggc
    10621 gacgtgcgtg atccgcgagc agagggaaat ggtgtgcctc acggaaaaat gccgtcagat
    10681 tcgtggtcag cgaattgata aacgcctgcc actcaccgct gtgctgatta gattccagca
    10741 agttcagata atgaccgaaa tccgtcagtc ccagcgaacg caaacgacga accagtcggt
    10801 tgtaaaccat gtcgcgttta tggtcagcca gaacgatccc ggctcgttga tagatcaatt
    10861 gacttatccg ccgaaaatgc gcgtcggaaa gcgccaggcg ctcggtcatc tgtaacaata
    10921 aagacgtttg cccacagggc agagatgaag tcatagcgcc ttctcaatca cttcaggata
    10981 ccactggcgc aatttgtaac tgcgccgact catgtcgtgc tacttcatgt tcttcaaggg
    11041 taaataccgc cacacgcgac gaaagatggt cggcctgatt cgccagttgt tccgttgcca
    11101 ccgccgcttc ttctaccagc gaggcgttct gctgagtcac ctgatccatc tggctgacag
    11161 cctgtgcaac ctgctcaatc ccccgctgtt gttcttccga cgcagaggca atttctccca
    11221 taatgtcgtt cacgcgggtc accgaactga caatatcgat catggtcgcg gcggcgttat
    11281 tcaccagttt cgaaccctgc tggacacgat tgactgactc ttcgatcagc cctttgatct
    11341 cttttgctgc ctgcgcgcta cggctggcaa gattgcgcac ttcacctgcc actaccgcaa
    11401 aaccacgccc ctgctctccg gcgcgagccg cttccactgc cgcattcagg gccagaatat
    11461 tggtctggaa agcaattccg tcgataacgc tgataatgtc gccaattttt tgcgagctgg
    11521 tggcgatctc ctgcatggtg tgagtcatgg tactgacctg aacaccgccc gcctgcgccg
    11581 ttgtcgcggc attttttgcc agttccgacg cctgtcgtgc gttatcggcg ttttgcccta
    11641 ccgtggcggt taattgctcc atactggcgg ccgtttgtgc cagcgatgcc gcctgctgtt
    11701 cggtacgact tgagagatcg ttattgcctg cgacaatctc cgcgatacca atgtgcatct
    11761 cctggcttcc cttacgcaca tcacttaccg tcccacgcaa agcctgctgc atggtcttca
    11821 gactggcaaa aatggcggtg atctcattac gaccatatac cgcaatcgga cgcgccagat
    11881 tacccgcagc aatgctgtca aaatggctac cgataatggc cagtggttga acaatcatct
    11941 tgcgcgtcca ccacagcgca ctgctgatgt agatcgccgc aacaataatc atgctgataa
    12001 acaccagtgc cgaaatctga tagttacgct ggctttgcgc actggcggct tccagcacat
    12061 ggttgatctc cagctgccag gcctcaaagt taacgtcaaa cgcattctgt gatccctgca
    12121 ccggcgcagt gaggaaatcc gaaagttgat tactttcaag ccaggtcgcc tgatgttcga
    12181 gatcgttgtg ccagcgggca aaacttttct ccgtctcttt ttgcaatccc ctgacgtgct
    12241 cgttgcccgc agtcatcgcc ataaaacttt taaacagcgt ggtggattgc gtcagactgg
    12301 cgcgcgccgt cgtcatcaac gttttaatgt catccgccgg atagctaagt gccgtcagag
    12361 tgcccgcttt gttcagcgcg gtactggcct gtaacattac cgcccgagtt tgcgctaatg
    12421 ccgcacgttg ctgattgctc tgctcgacct gattcagtcg ttgcaaatcg tcgcgaaatg
    12481 cccaaaaaga catgccgtta ctgccaatct gcaagatccc gcagagaatc aaaattaaaa
    12541 acagcgtggt cgaaattcga atacgattaa acatcaacgc tccccatcag gcggcaatga
    12601 ccgcgttagt aaatactcgt caaaatgttt cccagtttgg atcttgttca gcaattcgca
    12661 gtcgtggctg tgccggtggt tgctcactgg caggacggga tggtgtttgc ggtttattgg
    12721 tgagtgggct ggctgccaga cggaacgcgg aaaccgcttg cgttaaacga ctcgcctgtt
    12781 cttccagcgc agcggcggcg gcagctgatt cctgcaccag cgatgcgttc tgttgcgtga
    12841 cgcgatccat ttccgaaacc gccaatgcga cttgatcgat gccacggctc tgttcatccg
    12901 atgccgatgc aatctcgccc ataatgtcag tcacgcgagt gacagcattg acgatattgt
    12961 tcattgtttc cccggcgctt tcgaccagca ccgaaccggt atcaacgcgt gagacggagt
    13021 cttcaatgag ggctttgatc tcttttgccg cctgggcgct gcgactggca agattacgca
    13081 cttcacccgc caccacggca aaaccacggc cctgttcacc cgcacgcgcg gcttcaaccg
    13141 cggcattcag cgcgaggata ttagtctgga aggcaatacc gtcgataacg ctgataatgt
    13201 cggcaatttt cttcgaacta tcggcgatct catgcatcgt tttcactacg ccatccacca
    13261 ctttgccgcc gtgctgggcg gtgtcggagg cactttgcgc cagttgcgag gcctggcggg
    13321 cgttatcggc gttttgcttc actgtcgcgg tgagctgctc catgctggcg gcagtttctt
    13381 ccagcgcgga tgcctgctgt tcagtacggg aggaaagatc ggtgttgccc gccgcaattt
    13441 cacgggtacc ggcatagatg gcatctgaac cttcgcggac atgagtgacg gtgtcagtca
    13501 aagagcgttg catatgtgaa acgctctgcg ccaggtcgcc catttcactg cgcccgtcaa
    13561 tggtcagggt attcgccagg ttaccaccgg cgatttcgcg aatgtgagca ataatttttg
    13621 ccagcggagt aagcaacata cggcgaatgc cgtaccacgc caccagcaga atcaatacca
    13681 ccaccagcgc gataaccgcc agttgccact gggcaaatcg gtaatcatct gcgttgtcag
    13741 tgacgatatc gcgatacagt ttttcactgc tgagggcgta ctgagcaaac gcttcgccca
    13801 ttgcattttg cattccctgg gttggctgag cgaaataagc tccagtattg ccataatcta
    13861 gataatcaat cagttcagtt aacgctgtgt aatagttttt atatttttca tcaatattac
    13921 gactggtagc gaccatttca ggtaacggtg ccatgctttt gaatttttta taatgcgtcg
    13981 ctgcctgcgc caatgttttc ctggcgctat cgagcaattc aactttggcg ttactttgtt
    14041 gattggagga atccatcatc atccgtaccg ctgaacgact caggttaatg cgcgtttgca
    14101 gcattaaatc ccaggttgac gtcagctcgc cctgctgttc ccgtaattga ttggaaacca
    14161 caaagctctt ctggctatgg tgaagggaag aaaaaaacag actgccggaa ataagctgta
    14221 acagtgcgaa tacccccagc accattacca acagcgtgac tacgcggata cggttaatca
    14281 taaggcacct tcctgaaaac aagttgatct cgttatcggc aaggaggggg gaaactttat
    14341 tgctgatgcc acccgccgcg aaattgaaat aaaaaacccg atgcgcagat catcgggttc
    14401 atttcaattg aggaaatcgg gagaattacg ccacttctga cgccgcgcta tctaacagcg
    14461 ccatctcttc gctgttcagc agtttttcga tgttcaccag aatcaacatc cggtcgccca
    14521 gtgcgcccag tccagtgaga tattctgttg aaagcgtcac ggcaaattcc ggtgccggac
    14581 gaatttgctc cgccgtcaat gaaagcacgt ctgagacgcc gtcaaccacg atgccgacca
    14641 cccgctgtcc gagattcagg acgataacta ccgtgttgtc gttatagtcc acatccacct
    14701 ggctgaactt aattcgtaag tcaacaatcg gcacaataac gccgcgcaga ttcgtgacgc
    14761 ctttgataaa cgctggcgtg ttcgcaatcc gtgttacctg atcgtagcca cggatctcct
    14821 gcactttcag gatatcaata ccgtactctt catcaccaag ggtaaatacc agaaattcct
    14881 ggcctgacgg ctcgctggcc agctttgtta cattcgtcat accggtcata ttgttacctt
    14941 tttactcatt caggcggcgg tgttcgccat acgttgttcg cggtttatcg cctgcaaggc
    15001 ggagacatca acaatcagtg ccacgctgcc gtcgccaaga atggtcgcag cagaaatgcc
    15061 ggggactttg cgatagttac tttcaaggtt tttaaccaca acctggtgtt gaccaattaa
    15121 ttgatccacc agcaaggcgt agcggcgacc gccactttgt aagatcacca caattccctg
    15181 ggtggcttcg gttttcgcgc ccgcgacgtt gaacactttc cacagttcga cgatgggcag
    15241 atattcaccc cgcacttcca gcacccgctc gccgccggcc agtggatgga gatcggcttc
    15301 acggggttgc agtgattcca taacagcatt cagcggcaga atgaaaactt catccgcaac
    15361 gcgtacggac atgccgtcga ggatggccag cgtcagcggc agtaaaatgc ggatcgtagt
    15421 gccagtaccc tgcttcgact ggatttcgac atgaccgccc atctcctgga tattacgttt
    15481 aacgacgtcc atgccgacgc cgcgcccgga gacgtcggtg acctgctctg ccgtggagaa
    15541 gccaggtgca aatatcagca tcgcgacttc gtcgtcgctc atgttttcgc tgacagtcaa
    15601 accttgcgag gccgcttttg ccagaattcg ctcacggttt agccccgccc catcgtcggt
    15661 cacttcaatg caaatgttgc cgccctgatg ttcggcatac agaattaaat ttccgacgct
    15721 gtttttacct gcggcgagcc gtttttctgg cagttcaata ccgtgatcga ggctattgcg
    15781 taccaggtgg gtcagcgggt cgataatgcg ttctatcagg cttttgtcga gttcagtaga
    15841 actgcccacc agcgtcagtt ctacctgctt gccgagtttt cccgccagat cacgcaccag
    15901 ccggggatag cgactaaaaa catattccat cggcatcatg cgaatcgaca tcactgattc
    15961 ctgcaaatca cgggcgttac gttgtaactg ccccatgctg gttatcaaat caccatgatt
    16021 aaccgggtcc agttcgctgg aacgctgggc aagcatggac tgggtgataa ccagctcgcc
    16081 gacgaggtta attaattgat caaccttttc taccgctaca cggatgctgg tggattcatt
    16141 gctgcgcgtc gttttttccc gctccacgcg gccggttggc gcttgttcgg ctgccagttt
    16201 aagcactggt ggggtggata tttttggcga gacttctact gtttcaaagg taatctgatc
    16261 ggcttcaatc acaaaacaga gtaccgctgt gatgtcatct tcggcgatgt cgcccggtaa
    16321 tattgccgag agcgaatccg cccctttcac cacgtcagtt aacgttgtca gatgtcccag
    16381 ttcttcttcc agcaggtcga cttccccggc cttcaggcgc gaaaggataa ttcgtcgcgg
    16441 cgactgactg cgactctgct catcttgcgg ttcacttttg gcaaccacac ttaatcgggt
    16501 cactgcggat ggcgtttcgc ctttcgcttc taatgccagt tgacgcaagg cctggcagat
    16561 ataatcgaag ctggcggcat ccggctcttg cgactgttta taagcgtcga gctgttcttg
    16621 catgatgtcc ttcgtttcca aaaacagatt gataatgtcg gtgttgagtt gcatctcacc
    16681 tcgtctggct tcatcgagca ggttttccat cagatgcgtg gtttcctgca aaacgctgaa
    16741 gccaaaagtt cctgcccctc ctttgatcga gtgggcagcc cgaaagatgg cattcaattg
    16801 ttcggcatct ggcgcttccg gctgcaaaac cagcaaatgc tgctccatgt cagccaacag
    16861 ttcgtccgct tcatcaaaaa atgtctgata aaaatcgctt atatccatgc tcacgctgtc
    16921 acctcggttc ggctgatggc attgtgggaa cactgacctg tggtgcaacc tcaggttttt
    16981 ccagggcgct tactggctca ttctggcttt cggcgttttc atgcaaaatg gcctgttcgg
    17041 cttgtttgtt cagtaccagc aggctgatgc gacggttgac ggcatcatca ggtccgcgat
    17101 cgcttaagcg catcgttgcc gccatgccga cgacacgtaa cactttgccg ctatccaacc
    17161 ctccgaccat cagttcgcgg cgggatgcat tggcccgatc ggcagaaagc tcccagttgc
    17221 tatatccttt ctcaccgctg gcgtagggga aatcatcggt atgacctgaa aggctaatac
    17281 ggttgggaat accgttcagt acaggcgcaa tggcgcgcag aatgtcgcgc atatagggtt
    17341 cgacatcggc actgccggtt ctaaacatcg ggcgattctg gctatcgatg atctgaatac
    17401 gtagaccttc ctggaccaga tcgattttga gatggggacg taacgcccgc agtttcggat
    17461 cggactctat caactggtcg agatcacccc gcaatttccg caatcgactt tgctccatgc
    17521 gttttttcag ctcttcgatg ttcggctgct tattcacttc cccctggctt tgggtgtaat
    17581 catcaccacc gccgggaatt gggctttcac tattagaaat gcgatcgccg cccgtaaccg
    17641 cagtcgccag tggagtccgg aagtactccg caatctgaat cagctctttt gggctggaga
    17701 tggagatcag ccacatcacc agaaaaaagg ccatcatcgc agtcataaag tcggcataag
    17761 caatcttcca cgatccatgt gctgccccgt ggcttttggc tttgcgtcgt ttgacgacaa
    17821 taatcggatg cgcttgattc ttcatgcttc ctcggttgtc gtctgttgtt gcggattttt
    17881 caccgcacgc acatgctctt ccagttcaat aaacgacgga cgttcgctgg aatagagcgt
    17941 tttgcgacca aactcaacgg cgataggcgg tgcgtaaccg ttcagattag aaagcagagt
    18001 gactttgacg cactgcatca ttttgctggt ttcggcgctt ttctgacgta aaacagtcgc
    18061 taatggggaa ataaatccgt aagccaataa aatgccgagg aaagtcccca ccatcgcatg
    18121 tgcgataagc gcacccagct cggcggcagg acgatcggct gaacctaacg cgtgaacgac
    18181 ccccattaca gccgcaacaa taccaaacgc cggaagtgag tccccgacca gcgccagact
    18241 gttcgccggg acttctgcct cgctttcgtg cgtctcaatc tcttcatcca tcagagcttc
    18301 gatttcgaag gtgttcatgt gaccgctgat aatcaggcgc agataatcga cgataaaatc
    18361 aagcatgacg ctatccgcga ggatgcgtgg gtagctggcg aagatctcgc tctcacgggg
    18421 attttcaata tcacgttcca gcgaaaacat ccccatctgc cgcgatttcg ccatcaaccg
    18481 ataaagcaga gccagcagat ccatatacat tgctttggtg tatttggagc gacgaaacag
    18541 caacggcagc gccttcagcg tgcctttaat cgctttgcca ttattgccga cgataaacga
    18601 cccaatccct gcaccggcaa taatcaccag ttcagcgggt tgatagagtg ctccaaggct
    18661 tccaccggtc atcaaataac cgccgaaaac tgtaccgaga acaaccaggt aacctaataa
    18721 gataagcacg acatcatcct tccactgttg accatgacag gatgttcagt cgtcaggcgt
    18781 taacgcgcga ttggggcaaa aaaaagcagc ggtacgtcgt taccgctgct ggaatgttgc
    18841 gcctcaccgt atcagttaaa cagcctgtac tctctgttca tccagcagtt gtgggataat
    18901 atcggcagga ttctgggaaa gtttacgtct ttttactgcc cgggatggcg gttgacataa
    18961 gctgcaggca aagctgccaa caggctggtg agcgtgggta ataaaattgc cgccgcagca
    19021 gttgcagctg gaaagttgca gtaatccact ttcaacaaac cgcaccaatg tccaggcacg
    19081 ggttaatgcc agcagtggtc cttcttctgc ttgtgggcac tgttcaaggt ataaacggta
    19141 ggctttgatc accgcatcga cgccattaca caaaccggtt ttcagtaaaa actgccatgc
    19201 attacagaac atcgaagcat gaacgttttg ttcccaggtc ataaaccagt cggttgagaa
    19261 tggcagcatg cctttcggcg gtgggcttcc gcgcagttct ttataaagtt ttatcaggcg
    19321 tccgcgactt aactgtgttt cgctttccag catctgcaaa cgagcgccca gggtgatcaa
    19381 ttccattgcc agctgaatat cccgcgcttc ctgaacaatg cttttttcac tcatgatcag
    19441 gcccttttct tgcgcagcgc ttcttcaggc tgattaacat cattcagcaa gcgtgttgag
    19501 agcatgatgc cggtatgaat ttgctggaga tcgtcaacgc gggaatcttg cgtcaactga
    19561 gtaatcgtct ggtggctgtc aaaacggaag tgacaaacca gttgattggt ttctgccagc
    19621 ttaaccattt gcggaagagt cagtgccgct aacgttgtcg ccatttcttc atttatgccg
    19681 agacgaaaca tagcggacgc tttgtcctga acaatcaaac gctgtgcaag tagtaaatat
    19741 gacaagttga tgtcataaat gtgtttcagc aactcggagg tatgcattat tcccacccag
    19801 aataaccaac tttattttta tgcggtttca ccgcaccccg tgatgtcgcc gggaagcccc
    19861 ggtaaaaaat aattagcatt agaatagttg cgataagctg caataagcag aaccaccttt
    19921 ttggtttaat atgtccttac aaatagaaat gggtctttac acttatctaa gatttttcct
    19981 aaatcgacgc aactgtactc gtcactacac gcacatacaa cggagggggg ctgcgatttt
    20041 caataatgcg tgatgcagat cacacaaaac actcaattac ttaacataaa tgtataagtc
    20101 atacttttgt tttgggtgta tttcaatctg ttaaaaagtt tttcgctacg ctagcaagca
    20161 aaaatgaaac aggaataatc gaaatgggat gttgcgcaca gtcaaaataa ctcaccgtaa
    20221 ataatcatct gctataaata atcactttca tgcaatacca gataagctat ttttaaacag
    20281 acacttaccg cacaacaaac taattaaata caaagaaagg caacaatcaa cttaatgtta
    20341 atgaaatgtt ttacatatat taaccacttg tttgatgcgg a
//
LOCUS       DAJOWB010000084        19856 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJOWB010000084 DAJOWB010000000
VERSION     DAJOWB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.084"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            178..1275
                     /gene="ompC"
                     /locus_tag="OHO85_003240"
     CDS             178..1275
                     /gene="ompC"
                     /locus_tag="OHO85_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCQ2672583.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRT
                     DTQVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKA
                     QNFEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVD
                     YKINLLDKNDFTKALGVSTDDIVAVGLVYQF"
     gene            complement(1465..1848)
                     /gene="quuD"
                     /locus_tag="OHO85_003241"
     CDS             complement(1465..1848)
                     /gene="quuD"
                     /locus_tag="OHO85_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HCQ2672584.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(1866..2855)
                     /locus_tag="OHO85_003242"
     CDS             complement(1866..2855)
                     /locus_tag="OHO85_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCQ2672585.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPEHYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCAHWVLSVVRRDLDFNDNHAV
                     TMPELCWWLVRNDLADALPESAARKALRLPKSVLPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEQYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(2863..3660)
                     /locus_tag="OHO85_003243"
     CDS             complement(2863..3660)
                     /locus_tag="OHO85_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HCQ2672586.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSASEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(3680..4069)
                     /locus_tag="OHO85_003244"
     CDS             complement(3680..4069)
                     /locus_tag="OHO85_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCQ2672587.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACTAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKTAGAAA"
     gene            complement(4066..4392)
                     /locus_tag="OHO85_003245"
     CDS             complement(4066..4392)
                     /locus_tag="OHO85_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCQ2672588.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEVAGIIRALLAGEENARLRAAHWLH
                     ERGLKV"
     gene            complement(4392..4886)
                     /locus_tag="OHO85_003246"
     CDS             complement(4392..4886)
                     /locus_tag="OHO85_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCQ2672589.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSSSKLR
                     QSGRVAHRCEGDTRRHFPRLTEIPQEPEPQPVRESRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQQERNNFLARRELCLRKSSKRAVSGEEWYLSGN
                     YVGA"
     gene            complement(4883..5824)
                     /locus_tag="OHO85_003247"
     CDS             complement(4883..5824)
                     /locus_tag="OHO85_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2672590.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRRARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKTSRPDASQP
                     DTQTAEQDFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEHAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            complement(5814..5993)
                     /locus_tag="OHO85_003248"
     CDS             complement(5814..5993)
                     /locus_tag="OHO85_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCQ2672591.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            complement(6169..6720)
                     /locus_tag="OHO85_003249"
     CDS             complement(6169..6720)
                     /locus_tag="OHO85_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672592.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVEDVDNADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCVSE
                     SSDARECAAPGAVACRDCGETNA"
     gene            complement(6713..6973)
                     /locus_tag="OHO85_003250"
     CDS             complement(6713..6973)
                     /locus_tag="OHO85_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2672593.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIEL
                     AERLANFFKGEVSEMQILYPARFQSSQNQNGFKPQEQEVNRG"
     gene            7071..7763
                     /locus_tag="OHO85_003251"
     CDS             7071..7763
                     /locus_tag="OHO85_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2672594.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQTLSNIAKSLGVDIADLFTSAHKSNTVYKNSNNEDVAQVKDVFRIEMLDISASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDVS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            complement(8083..8598)
                     /locus_tag="OHO85_003252"
     CDS             complement(8083..8598)
                     /locus_tag="OHO85_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672595.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGSYQEINASLKAKIAEFENFEAQTEGYILNQLESGTFVYSKEVIVN
                     GGSITMHLCPKCFGQKIVSILQPFPVSEDELFHKSRCLHCENKFLMNKNPDYVSPPSI
                     EELSRKLNGNL"
     gene            8743..9054
                     /locus_tag="OHO85_003253"
                     /pseudo
     CDS             8743..9054
                     /locus_tag="OHO85_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9069..9431
                     /locus_tag="OHO85_003254"
     CDS             9069..9431
                     /locus_tag="OHO85_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672596.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            9497..10321
                     /locus_tag="OHO85_003255"
     CDS             9497..10321
                     /locus_tag="OHO85_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HCQ2672597.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            10449..10985
                     /gene="yfdR"
                     /locus_tag="OHO85_003256"
     CDS             10449..10985
                     /gene="yfdR"
                     /locus_tag="OHO85_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCQ2672598.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNDLSELRKCA"
     gene            10976..11338
                     /locus_tag="OHO85_003257"
     CDS             10976..11338
                     /locus_tag="OHO85_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672599.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLLRKFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            11338..11643
                     /locus_tag="OHO85_003258"
     CDS             11338..11643
                     /locus_tag="OHO85_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672600.1"
                     /translation="MTTFTNKELIKEIKERISSLEVRDDIERRAYEIALVSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAVIHFSTMTQQEVK"
     gene            11870..13033
                     /locus_tag="OHO85_003259"
     CDS             11870..13033
                     /locus_tag="OHO85_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCQ2672601.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVRAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(13049..13125)
                     /locus_tag="OHO85_003260"
     tRNA            complement(13049..13125)
                     /locus_tag="OHO85_003260"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13089..13091),aa:Arg,seq:tct)
     gene            13368..14000
                     /gene="fimZ"
                     /locus_tag="OHO85_003261"
     CDS             13368..14000
                     /gene="fimZ"
                     /locus_tag="OHO85_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HCQ2672602.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSKTLNYIKSNKCSTNSSTITVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(14003..14518)
                     /gene="sfmF"
                     /locus_tag="OHO85_003262"
     CDS             complement(14003..14518)
                     /gene="sfmF"
                     /locus_tag="OHO85_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HCQ2672603.1"
                     /translation="MRRALFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(14529..15536)
                     /gene="fimH"
                     /locus_tag="OHO85_003263"
     CDS             complement(14529..15536)
                     /gene="fimH"
                     /locus_tag="OHO85_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HCQ2672604.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYILMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(15549..18157)
                     /locus_tag="OHO85_003264"
                     /pseudo
     CDS             complement(15549..18157)
                     /locus_tag="OHO85_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            complement(18188..18880)
                     /gene="fimC"
                     /locus_tag="OHO85_003265"
     CDS             complement(18188..18880)
                     /gene="fimC"
                     /locus_tag="OHO85_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCQ2672605.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(19100..19642)
                     /gene="fimA"
                     /locus_tag="OHO85_003266"
     CDS             complement(19100..19642)
                     /gene="fimA"
                     /locus_tag="OHO85_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCQ2672606.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT         5434 a         4836 c         4489 g         5097 t
ORIGIN      
        1 tcaaataaga gccataaata ggtttcgata gaaaaagttc agataaaaat agagatctac
       61 ttcacaaatt aaatgagaaa ctaaaactta catcttgaaa taatcacatt gattagatga
      121 atatttatcg cgcagtgaca tcatttttta ataatagttc aaaaaaaagg gctcacgatg
      181 aaaaaattaa cagtggcaat ttctgctgta gctgcatcag tactgatggc gatgtctgct
      241 caggcagctg aaatttataa taaagacagt aacaagctgg atctgtacgg gaaagttaat
      301 gccaagcact acttttcctc taacgatgca gatgatggtg atactactta tgttcgtctg
      361 ggcttcaaag gcgaaaccca aatcaacgat cagctgactg gtttcggtca gtgggaatat
      421 gaattcaaag gcaaccgcgc tgaatctcaa ggttcctcca aagacaaaac ccgtcttgca
      481 tttgcaggcc tgaaattcgg tgactacggc tcaatcgatt acggccgtaa ctacggtgta
      541 gcatacgaca tcggtgcgtg gactgacgtt ctgccagaat tcggtggcga tacctggacc
      601 caaacagatg tgttcatgac tggtcgcacc actggtgttg caacctatcg taacaacgac
      661 ttctttggtc tggttgatgg tctgaacttt gctgctcagt accaaggcaa aaacgatcgt
      721 agcgatttcg ataactacac cgaaggtaac ggtgatggct tcggtttctc tgctacctat
      781 gaatacgaag gatttggtat cggtgcaact tatgcgaaat ctgatcgtac cgacactcaa
      841 gttaatgcag ggaaagttct tcctgaagta tttgcttccg gtaaaaatgc agaagtttgg
      901 gccgcaggtc tgaaatatga cgctaacaac atttacctgg ccactaccta ttctgaaacc
      961 cagaatatga ctgtatttgc tgatcacttc gttgctaata aagctcaaaa cttcgaagct
     1021 gttgcacaat atcagttcga tttcggtctg cgtccgtccg ttgcttacct gcaatctaaa
     1081 ggtaaggatc ttggagtatg gggcgatcag gacttagtca aatatgttga tgtaggtgca
     1141 acctattact tcaacaaaaa tatgtctact ttcgttgatt acaaaatcaa cctgcttgac
     1201 aaaaatgact tcactaaagc actcggtgta agcactgatg acatcgttgc tgtaggtctg
     1261 gtttaccagt tctaatctga ttacgaaaaa gatatgttgc gggaggcgtt gcctccccaa
     1321 catataagtg gctccctcaa gccacttcct ttaggagcac aaccttgctt ctaactatat
     1381 aaaccttctg ttatatatta ccctttattt ttgggggcgt tgcaacgccc catttttaat
     1441 aactttcagt aaacaattgg catattaatt agagttatta acaacgatat ccatctctaa
     1501 ccgaatatct aatgccatta acatcccttc aattatgccc tcagccttct gtaacctttt
     1561 cccgatataa ccatcagagc agcaatgctt acctgccagt gacatgaatg tcataccgac
     1621 tacataataa tctactaata aatcgtgcaa atcgctgttg ttctttttca gacgggccat
     1681 gcacccgcaa atgatcatcg cgtcatcgtc acaacattgc gggcgagatt ttacttttga
     1741 aggaattaat cccttaaaac cggcggcaat ggacgaccag gtcacatctt catgattatt
     1801 agccgcccac gctccccaac gttcaagaac catctgaata tcacgcatta actttctcca
     1861 caaaatcagg ccagcacacc aatcgccagt gcgcgatcga taaaacgaaa tatcagctcc
     1921 agctgggagc catacttctc ttcaaatgcc acggtatccg catgcagttc gtcgtggtgt
     1981 tttctgcaca aaggcagcac aaaaaggtca tgcgcttttg tacccattcc accctgacca
     2041 tggcctatca gatgatgcgg atcatcagct ggctttccac aacatgcaca cggctgcgtc
     2101 ttaacccagc gcgtgtactg ttcattaacc cagcgacgac gtttggggcg taacataaaa
     2161 gactccggcg actccggatc cactttcagc gccagcacct ttttcgcttt atcctggata
     2221 atgctggtgg caggaaccga aggcacaagg tcactttccc gggtgacaga cggcaaaaca
     2281 gacttcggta atctcagtgc cttacgggct gcgctttccg gtaaggcatc cgccaggtca
     2341 ttacgaacca gccaccagca cagttccggc attgtcacgg catggttatc attaaaatcg
     2401 agatcccgac ggactacaga caacacccaa tgggcacagt tatccgttgc cattgattcc
     2461 agccgctccg tgaactgatc acgtaactga ttatcgcagt gccagcacag acggattgcg
     2521 cccggcgcgt gtcgcattgt ggtcatgttc tcgctgtgcc agtcggaatg aggccactgg
     2581 cagccttttt cacgaagtaa ccagctttca agacattcca ccccaccagc acgacgaatc
     2641 actgcctcat tacggaacac ggcccgaacg gcaggatcat ccgccagcgg ttgtgatgcc
     2701 gcgggaacgg caccactggc gaaagatgaa taatgctccg gctcaggctc cagcagtaca
     2761 cgcccctgca taaacagggg catcagctct gaaccgggcc tgaacaatac gatccccata
     2821 cgcggggcaa tttcaggggt cagtagtgct ctcacggtca cctcaatgaa cggtatcgag
     2881 cagctttaac agctcaggga atcgggattc gaagaaatgc ggctgcgtct cgcgcggatt
     2941 tgcgggactg gtgatgttct tgccgaacat gcagcctttc gctgtcagcg accagaattt
     3001 tttgatgttg ttaatcgcgg tacggctgta tcgttcgcgc tgctcgacga tccccagctt
     3061 caccatctgg tgatatgcct gattagctgt caggcggata ccatactgct tcagcagtgc
     3121 actcagtgac agcgtgggac ggcttgagcc atcaggcgcg tcagcaggag catcaatggc
     3181 atagcgcggt gccagattcg gtaagccaac agcctcctgg agtttctgac aggcaccaag
     3241 cactgaagag ttagacaggt ttaactcccg gcgcataaag tccagcagaa tcacgccagc
     3301 ctgcatcttg tcagcagcct gcccggataa tttttccgat gcgctggtta ccatatcgaa
     3361 agtacggatc accttcagat ggaatgacgg gctgatccac attgcatagg catacaccag
     3421 ttctttgcag acatacgtcc cctggttatt tccgccacga ataacgttaa ctggctctat
     3481 attgaccgag ttgcaaatct gcaactcgct tattaaacgt tcagtttgct cattgcggag
     3541 ccagaatgca ggcttatgct tatccagaga accggcagcc ctgtgcagat cgttcaggct
     3601 gtaacgccca taagcatcac gacgaacttc aataccatca atgaccatca gattattcat
     3661 acttcgtttc tcctcttgat caggcggctg cacccgccgt tttctcgtac ttactgatag
     3721 tgatctcgac cttcccttcc gggataaccg gtccccactc caccagcatt cttttcacct
     3781 ggctgtcgtc ttcccacaca cccgcgtggg tcagggcgtc aaacagcgcc ttgttatagt
     3841 tgtccagatc gcggatccgg ttatccggag gaaacaacac gatctccact gaagcaggtg
     3901 ccgacgttgg tttcggcaga cgacgtaact gctcaactat tgctgtgcac gccgcactct
     3961 ggaattttcg ccccgccgcg cttatcaggc tcttaccagc aaatgcccct ttgttggggt
     4021 gtcgccagta ggtgttcacg ctgggtggaa aaggcagaat cagcttcata ctttcagccc
     4081 cctctcatgt aaccagtggg ctgcacgcag cctggcgttt tcctcaccag caagcagtgc
     4141 gcggataatc ccggccacct cgctgtcgtc gtccttcacc gcggtatgaa gcgtgatgcc
     4201 ccgggccacg ccacgcttta tcgtgatgac gccttttttc tccagtgcgc gaagatgctc
     4261 caccgctgca ttcactgaac ggtatcccag catggtagcc acctcctgat tggttggcgg
     4321 gaagccacgt tctttctggt aagaaatcag catatccagc acctgctgct ggcattgagt
     4381 taacgtcgtc attaagcccc cacgtaattc cctgacagat accactcttc acccgataca
     4441 gcgcgcttgc tgcttttccg taaacacagc tcacgacgcg ccagaaaatt gtttcgctct
     4501 tgctgggagt ggctttcacg gaatgccgcc atccacaccg ttgcagcacg acggtataag
     4561 cccctggact ccagttcttc cgcctggcgg gtcaggcaca aaatcacccg gggatcgtta
     4621 gtgccgacat agaaattgcg cacaggtctg gattcacgaa ctggttgcgg ttccggctcc
     4681 tgcggtatct cagtcagccg cgggaaatgt ctgcgtgtat ccccttcaca acggtgagcc
     4741 acacgcccac tctgacgtaa cttgcttgat gactgcagaa cgcgctgtcg tgagtaacct
     4801 gcaaaagcat ccgcaatgtc tccggaagta caccccggat gggcttcaat gaatttctga
     4861 acgtcattca aaagactcat gatcaccccc tgaatcctgc cgggatctgg ctgtagtcca
     4921 cgttgtcgta actggctttg aagtacgggt cttcgcgttt ttcggtgtac gtgctgacgg
     4981 acggcgataa gcgcagggaa agctcatccc atttttcccg cagcttcgac gggctgagca
     5041 cgttacggca ccagaacgga tcgcgactga cgcggctgta catctcgcag atttgtttat
     5101 gagtacgacc atcctgcaca cacatcaggc gaatttcgtt tgcccaggct gtccagttcg
     5161 gttctttggg acgaaccacc tcgccgtcac attcggcggc atgctcgtac agggcgatga
     5221 tttttttcca gagccactgt gcgcaggtca aatcatcctg cgtcccccac tggcgctttt
     5281 tagggctgaa tacaaccgca tcaggatggc gagttaaaaa atcctgttca gccgtctgcg
     5341 tgtccggttg cgaagcgtcc ggacgagaag tttttttatc tgacggatca tgttttgatt
     5401 ttactgacgg atccccacca gattctgacg ggtgaaaacc cgcttttttg ccagatttcg
     5461 acgcatcaaa ttttgacggg tcagattttg atgcgtcaga ttttgacggg tcagaatctg
     5521 acagttgaga aaatgccgct gcctgaagct tcgcaacgtt aagctgataa acattcgacg
     5581 cattgcggtt accctggcga cgcgccctac gcgttaacca gccttctgct tccagccgtg
     5641 cgatagccgt tctgacggta ctcatccccg cgccaatctg gcgggcaatg gtttcaattg
     5701 atggccagca cacaccttcg tcattactga aatcagccag gcgggccata attgccacgc
     5761 tggataattt catgcctgat gcagcgcaac catcccatac atagccggtt aatttagtgc
     5821 tcatgaccga cctctatttc cctgaattta cgacgaaact gttcgagcgg gctgaagcac
     5881 tcatgctcat agccttcgcg gaggtagata acacgttgtg tttccggctc ccaacgaatg
     5941 actctgacgg gcactccgta gtgatctttg aaccagcggt taacttgtcg caaaggactg
     6001 tctccttctg ccggttgaaa tcacccacag cccactctgc aaagctgtgg gttacaattt
     6061 ccctgtcacc tggtacattt actgcatagc aatactccac cttcgctttt ccacccggta
     6121 caggaagcgc aatcagttgc gagcgacggt agtgtgttgt taaactgttc atgcgttagt
     6181 ttctccacag tcacgacacg ccacggcgcc cggagctgca cactcgcggg cgtcactact
     6241 ttctgaaacg caaaagattt tgtagaccag tgctgcatgc tcctgcagct tcgaaattga
     6301 gaggtacagc tcatcgttaa ttgctgtctt ctcatgcggt tccactacac cgtcttcaat
     6361 tgctgaacga atctgttttg aataactgcc gatctgttca atgacttcca gcaggcgttg
     6421 gttgatatcg gcgttgtcca catcctcgac atcaggaaga gacacaaaga cgccatttgc
     6481 agactgcgcc acagcgtcag caatgaagtg agttccacca gcacgttgca aaatcattgc
     6541 ccatcccagc gggaaaatct gatcgccatc ggcacgaagg cggttaaata atgcgttctc
     6601 tgttacatcc agccagtcag cagcttcagc gtaccccccc ggcaacgctg cgatagtttt
     6661 tctgacagct ttcacgtacc actcaggctg tttttctact ttccagtgat gcttacccac
     6721 ggttcacctc ctgttcctgt ggtttaaacc cattctggtt ttggctagat tgaaaacgtg
     6781 ccggataaag aatctgcatt tcgctgactt cacccttaaa aaaattggct aaacgttctg
     6841 caagctcgat agatggaatc tgctccagcc tctcaatacg actcaacgtc gctggattga
     6901 cttgaacacc cgcagcaaca tgctgcaaag tgaaaccatg cgccttacgc acatttcgta
     6961 atggtgattg catatgccct ccaaatattg cgcgttatgc atgttatttc acgcaattat
     7021 tttgcgcaag ttgatttgct tatcacgcaa taaagaaatg taataaacgc atgaacatag
     7081 gaaaccgagt cagacaactt cgccaagcga agaacatgaa aatcgccgat ctcgctgaag
     7141 caataggagt agatgcggcg aacatctcgc gcttagaaac gggtaagcaa aaacaattta
     7201 ccgaacaaac actgagtaat attgccaaga gcttaggtgt tgatattgct gatctcttta
     7261 cctctgccca caaaagtaat actgtatata aaaacagtaa taatgaggat gttgcgcagg
     7321 tgaaggatgt gttccgtatt gaaatgctgg atatcagtgc cagtgcggga aatggcctta
     7381 tccagggcgg tgatgtcatt gatgtgattc atgccatcga atacagaact gataatgctg
     7441 tatcaatgtt cggcggacga ccagccaatc acatcaaagt tatcaacgtt cgtggggaca
     7501 gtatgtgtcc aaccattgag ccaggagatc tcatcttcgt tgatgtcagc atcaatcagt
     7561 ttgatggtga tggtatatat gtctttggtt ttgatgacaa aatatacgtt aaaagacttc
     7621 aaatgattcc tgacaaactg ctggtgattt ctgataacca gatttaccgt gaatggggaa
     7681 ttactagcga aaacgaacac cgattcatgg tctttggaaa ggtcttaatc agtcagtcgc
     7741 aaacccttaa gagacataat taacctcaat atcccatcca tcggccaccg aaaggtggct
     7801 ttttactacc tataaatttg catacctcgc aaatatcact tgcatatctc gcaatttaat
     7861 tttatctttt gttccagacc aactacagga ttacaacaaa atctggttgc aacacggtgc
     7921 atgtgtcgta agcagtcagt aaatgtcaaa aacgaacagg caggacgccc acgaagtagc
     7981 cgcctggggc atatgaagtc caggatgatt cgttagcaac aaaaaagcgc cctacaggac
     8041 gcttagctct ttagcaatct ggatatccac aacagtagta atctacagat tgccgttaag
     8101 ttttctggac aactcctcaa tggatggagg cgatacgtaa tccggatttt tattcatcag
     8161 aaacttattt tcacagtgga ggcacctgct tttatgaaaa agctcatctt cgctaaccgg
     8221 gaatggttga agtatcgata ctatcttttg tccaaaacat tttgggcaaa gatgcatggt
     8281 tatgctgcca ccgttcacga ttacctcctt cgagtataca aaagtacccg actcaagttg
     8341 gttaaggata tagccttccg tctgagcctc aaagttttcg aattctgcaa ttttagcttt
     8401 gagagaagca tttatttctt gataagagcc caccagttca acgagagaca cgcattcgcg
     8461 ctgaatagac gcaagctttg agttcagctc accaatagcc gcatttactt cagcttgagt
     8521 ttttgcctcg ttcattagtt ttgcaatctg ggctgtttca cgaatagccg tcattgctgc
     8581 cgttaattca gcgatcacat tgaatactct tattgttgtt ggggatatcc agattaaccg
     8641 aatccttgtt gttggggaat aaccaggtcc acctcgcctg atgtggctaa aagcaggcac
     8701 ataacagcta agtattttca accagagaga atccttagcg ttgtggtgaa tgcggctcag
     8761 cgcacgcggg ttaaggttga ggctgacagt cgaccttctg tggataccca cccgcctggt
     8821 gtgcaacctt cgccaggcac cgggaggcac ccggcaccac aactttatgc tgtgtgtagt
     8881 cctggcggta ccagtttgta cccttgcttc cggctggtac cgtccttttt tgcaaaacag
     8941 agaagagcat caccggacga cgggctcata acccaatcca tccgggcggc tgccaccgca
     9001 ggtgttcttc tctgttttgt ggagaaacca accgaccttg cagggtcgat atgatgagga
     9061 gcagcaaaat ggctagcgaa cgcagtactg atgtgcaggc atttatcggg gagctggacg
     9121 gcggcgtatt tgaaaccaaa atcggcgcag ttctcagtga ggtcgcttcc ggtgtgatga
     9181 acacgaaaac caaagggaag gtctcactca atctggaaat cgaaccgttt gatgagaacc
     9241 gtgtgaaaat caaacacaaa ctctcatatg ttcgcccaac taaccgcggg aaaatttccg
     9301 aagaagacac caccgaaacg ccgatgtatg tcaatcgcgg tggtcgcctg actattctgc
     9361 aggaagacca gggacaatta ctgactcttg ccggtgaacc tgacggaaaa ctccgcgcag
     9421 caggtcatta gtatcgtttt taataaactg attatttctc tcatcactga atatttttat
     9481 atagtgagga cttattatgt ctcagaactt agacgcaacc gcaattaatc aaatccatgc
     9541 ccttatttct gctcagggtg ttaatgaaat tatcagtaag attggtgccg atgctgtggc
     9601 attgcctgag aatttccgca ttcatgatct ggaaaaattt aatttaaatc gcttccgttt
     9661 ccgtggtgcg ctttccactg ccagcatcga tgactttacc cgttattcta aagatcttgc
     9721 agatgaaggc acccgctgct ttatcgatgc cgataatatg cgagccgtca gtgtgcttaa
     9781 cctgggtact attgatgaac caggtcacgc agataacact gccactctca aactgaaaaa
     9841 gacagcaccg ttctctgctc tgttgtctgt taacggcgag cgtaactccc agaagtcact
     9901 ggcagaatgg atcgaagact gggccgacta ccttgtgggc tttgatgcta atggtgacgc
     9961 cattcaagca acaaaagcgg ctgcggcagt ccgtaaaatc acgattgaag caaaccagac
    10021 cgctgatttt gaagataatg acttcagcgg caaacgctcc ctgatggagt ctgtcgaagc
    10081 gaagaccaaa gacattatgc cagtggcatt tgaatttaaa tgcgttccgt ttgaaggtct
    10141 gaaagaacgt ccgtttaaat tacgcctcag cattatcact ggcgatcgtc ctgtactggt
    10201 tctgcgcatt attcagctgg aagcggtgca ggaagaaatg gctaacgaat ttcgtgatct
    10261 gcttgttgag aaattcaaag acagcaaagt agaaaccttt attggtactt tcaccgcctg
    10321 atttcattac tgcaaatgcc cctgcggggg catttatgga aacgtaatta actcaataat
    10381 caccggatgg tgagggcttc cttttaccag aattcagcgc ggtgcagtgc atatacgtgg
    10441 agaacaaaat gtcatttatt aaaacttttt ccgggaagca tttttattat gacaagataa
    10501 ataaagacga catcgatatt aacgatatcg cggtttccct ttcaaatatc tgtcgctttg
    10561 ccggtcatct ttcgcacttc tacagcgtcg cccaacatgc ggttctttgc agccagctgg
    10621 tgccgcagga atttgctttt gaagcgttaa tgcatgatgc aacagaagcg tattgccagg
    10681 acattcccgc accactgaaa cgccttcttc ctgactataa acggatggaa gaaaaaatag
    10741 acgccgtaat ccgtgagaaa tacgggttac ccccagttat gagtacaccc gtgaaatatg
    10801 ccgatctcat catgctggca accgaacgcc gcgatctcgg gcttgatgat ggctctttct
    10861 ggcctgtact ggaaggcatc ccggcaacag agatgttcaa cgtgattcca ctggcaccgg
    10921 gtcatgccta cgggatgttt atggaacgtt ttaacgattt atcggagtta cgcaaatgcg
    10981 catgaatgtt ttcgaaatgg aagggtttct tcgcgggaaa tgtgtaccgc gagatctgaa
    11041 agtgaatgaa acaaatgctg agtacctgtt acgtaaattc gacgcgcttg aagctaaatg
    11101 tgcggcactg gaaaacaaaa taataccagt gtcagctgaa ctgccaccag caaatgaaag
    11161 tgttctgtta tttgatgcta acggagaagg ctggctgatt ggctggcgtt ctctctggta
    11221 cacctgggga caaaaagaaa ccggagaatg gcagtggaca tttcaggtcg gggaccttga
    11281 aaacgtcaat atcactcact gggcagtaat gccaaaagca ccggaggctg gagcataatg
    11341 accacattta ccaataaaga actgattaaa gaaatcaaag aacgaatcag cagcctagag
    11401 gttcgagacg atattgagcg ccgtgcttat gaaatcgcac tcgtatctct ggaagtagag
    11461 ccagatgaac gcgaagccta tgaattattc atggaaaagc gtttcggtga cttagtagat
    11521 cgtcggagag caaaaaacgg cgataacgaa tacatggcat gggatatgac tctcggttgg
    11581 atcgtctggc agcaacgagc tgttatccat ttttcaacaa tgacacagca agaggtgaaa
    11641 taatggagcc atacagcctc acactcgatg aggcctgtca gtttcttcag atatccagac
    11701 caaccgccac caactggata cgaacaggcc gcctacaggc aacacgtaaa gatccaacca
    11761 agccaaaatc tccttacctc acaacacggc aagcctgcat tgcggcgctt cagtctccgc
    11821 tgcatactgt ccaggtgagc gcgggtgatg gcataacaga ggaaagaaaa tgtcactctt
    11881 ccgcagaaat gaaatatggt atgcctcgta ttcgctcccg ggcgggaaac gaattaagga
    11941 atctcttggc acaaaggaca agcggcaagc tcaggagttg cacgacaagc gaaaagcaga
    12001 actctggcga gtagaaaagc taggggattt acctgatgtc acttttgaag aggcctgcct
    12061 aagatggctt gaggaaaagg ctgataaaaa atctctcgat tcagataaaa gccggattga
    12121 gttctggctt gaacattttg agggtataag gcttaaagat atctcggagg caaagattta
    12181 ctctgctgta agcagaatgc ataacagaaa gacgaaagaa atatggaaac agaaagttcg
    12241 ggccgccatc aggaaaggta aagaaccgcc tgtttatgaa ccaaagccag tatcaactca
    12301 gacaaaggca aagcatcttg ccatgataaa ggccattctc cgtgctgcag aacgcgactg
    12361 gaagtggctg gaaaaagcgc ctgtcatcaa gataccagcg gtcagaaaca agcgagtcag
    12421 atggctggaa aaggaggaag caaaacgcct tattgatgag tgccccgaac cactgaaatc
    12481 tgtcgtcaag tttgcgctgg caactggtct gagaaagtcg aacatcataa atctggaatg
    12541 gcaacaaatc gacatgcagc gacgagttgc ctgggtgaat ccagaagaga gcaaatcaaa
    12601 ccgcgccatt ggtgtggcgc tgaacgatac cgcctgtaaa gtgttgcgtg atcaaatagg
    12661 caagcatcac aaatgggtgt ttgtacatac caaggcggct aagcgagcag atggaacatc
    12721 aacgcctgcg gtcaggaaga tgcgcatcga cagcaagaca tcatggctat cagcttgtcg
    12781 tcgtgcagga attgaagatt tccgtttcca tgacctcaga cacacctggg caagctggct
    12841 gattcagtca ggcgtcccat tatcagtgct tcaggaaatg ggcggatggg agtccataga
    12901 aatggttcgt aggtatgctc accttgcgcc taatcatttg acagagcatg cgaggaaaat
    12961 agacgacatt tttggtgata atgtcccaaa tatgtcccac tctggaatta tggaggatat
    13021 aaagaaggcg taactgattg aattgtaatg gcgcgccctg caggattcga acctgcggcc
    13081 cacgacttag aaggtcgttg ctctatccaa ctgagctaag ggcgcgttga taccgcaatg
    13141 cggtgtaatc gcgtgaatta tacggtcaac ccttgctgag tcaatggctt ttgatctggt
    13201 tgctgaacaa gtgaacgacc gcgtctgatt ttctgattta tttcgctata gcggcaaaca
    13261 aacacacacc gctgcgcgtc tgaatcaaga aaacccgtat tttcatgtat caaagtacaa
    13321 tttaccgccc taacggaaaa ttgtccgctc ctatgagact ggtaactatg aaaccaacgt
    13381 cggtgatcat tatggatact catcctatca tcagaatgtc tattgaagtt ctgttgcaaa
    13441 aaaacagtga attgcagatt gtcctgaaaa cggatgatta ccgcataacc atcgattatc
    13501 tccgaacccg tcctgttgat ttaatcatta tggatataga cttgcccgga acagacggtt
    13561 ttaccttcct gaaaaggatc aaacaaatcc agagcacagt gaaagtgtta tttttatcat
    13621 cgaaatcaga atgcttttat gctggcagag cgatacaagc tggtgctaac ggttttgtca
    13681 gtaaatgcaa tgatcagaat gatatttttc atgccgttca gatgatcctc tccggataca
    13741 cgttttttcc cagcaaaacg cttaactata taaaaagcaa taaatgtagt acgaatagtt
    13801 caacgatcac tgtgctatct aatcgtgaag tgaccatatt acgttatctg gttagcggat
    13861 tatctaataa agaaattgcc gataagttat tacttagcaa taaaacagtt agtgcgcata
    13921 aatctaatat ttatggcaag ctaggtttgc attcaattgt agagcttatc gactacgcca
    13981 aattatacga attaatataa tattaattat aattgatcat aaatatcgca tccgctttcg
    14041 ccacacctgg ccgaacaccg ctggctaacg ctcgataatt ggcaaaaaag tttagtgtga
    14101 cattgccatt tgcatctact tcctcagtcg ggctcgcctc ccccagtgcg agccgggagc
    14161 gatcgctatt acgtaattcg atggcgacgg tttgtgccat tgcgggatca tccagagcca
    14221 gcaggttggt atcggatgcc ggcgttcccg taaataaaat cgcaactgaa cccggaggac
    14281 atccctccag ccgcaggcta aaagggacga gtgccgtggt atcgccagcg ttcagtaatt
    14341 gtgtcgtagg ccatctgcct aaatctaccg tcttatcaat atccgcagtg tttacggtac
    14401 aggagaaatc aacaacgtta ccgtgcaaat tgatattaat cgttcccaaa gggtcagctg
    14461 cccatccact ggaactccac agtaacccgc agaaacagct aaagagtgct cttctcattc
    14521 tccgtacctc attgataatc gacgcgtaaa taccccagcg cgctaaacgg cccttcggtc
    14581 ggtttttgac cggtaatact gataggccag gcgcgaagtg tgacattggc tgccgcagct
    14641 gcatccagac ggaaaggaat aacgctattg agatcgttag gcgtgatcgg cgtatcgttc
    14701 tgatcggcga caataaaacc taaatcctga ttgtccgaca ccatcgcctg accagaaacg
    14761 gcactggctt ccagacgcat tgttaaataa gcctgcgcag caacattcgt acatttgacc
    14821 gcaatgctct tggtttgcgg catgacacca gcaggtcgat tacccggccc tgccgcacta
    14881 aataacgatg cgccgatatc accaaaatca aattcaacaa tctgcccggc atttaattcg
    14941 cagttttgcg gtacttcaac ccggccacca aaactaatgg tataaacagg tgtaaccaac
    15001 ggatcgtaag gcgttgatgt cacatacacg gtaaacatca cctgtctggg gatctccacc
    15061 atgttaataa agggacgaat aacctttaaa cgaaatatta aacgagaatc catcaccccg
    15121 aatggaaggt tttgcgaaac gttaggatct gttcccatca gaatataatt ttgcgggggg
    15181 aactgaatat ccatcacact atcaaccaga ctcatcgcac ctaatagata atcatttaat
    15241 tgcatatatt tataattatc gatagtttcc tgaacaataa agttggtgac ataactacgg
    15301 tatgtataat tcaccagcgt accgggtgga caaattgctg agacacctac ccagccggat
    15361 ttttccggta aaacaacaat ctgccctggc tggttattgg tgctattaaa gatatttgag
    15421 agatcatagt gttcatcttc tgctacgcca gtactatttg cacaaacagt agcccaggat
    15481 atgtttgcca gacacaaaca tgccatcgcc aataataatc tgcagattat cttcattgat
    15541 aatttccttt atgagccagg atgtgtgcaa actgccgaaa taacagtgac ggcttgctgt
    15601 aagctttgct tcggcaatac atagtgcgca atgcagcgtg agttcgcgcc gtctccccac
    15661 tgaaccagta attcaccaga caatggcgca ccacttaaat aaacttgccc gtcatcaccc
    15721 acatactggt tattccgata ctgttttccc gtaccagtga tccaaacggc accggttttc
    15781 cgccctgggt aacggtaatg agcgcccgca cgcctatgcg ggtatcaaaa ttggcacgaa
    15841 ccaacgcgcc ttgcgtcggc acaacgctgc taatattttt ttcaacatcg atggaattcc
    15901 ccatcgtatt ggtatcaagc gcgatacggt tataccgata aaccgtggca tacggcatca
    15961 ccgcatagcc gcgccagtcg gttaaaatgc cagtttgatt ttcaatgcgt acaccgcctg
    16021 cgccaggcgc tttaatcaaa acattggtat ccccaaaagg ctggctcagc gttatgccat
    16081 tttcatgccc gaccacaccg ccagacagct gccagttaac gtcatgttga tcgcggtcgt
    16141 agttataccc gacccccagc gtagcgtaag cggcctgcca gtttgccgtg gcgctgcccg
    16201 tatacccatt attcgaggta tcaccctggc ttacgtgata actcaggttg tggccttcca
    16261 gtaaggtccc acctacacct gccagccagc tattttgccc gttgctgtta cggttggcgt
    16321 taaaggaggc ataagcgcgg tcgagcgcat tttcccgggt gtagcgacgt ttggtcagaa
    16381 cattgaaagg cactgaaaca ttaagtccga caatacgttc gttatcgggg atccctacag
    16441 attcattcca cgaaaatgag agtgaataac tgatgccaac ccagctgctg gtatacccca
    16501 cctgatacca ggtatctgaa tccgaagtat tccagtattt ttgatgggta ccagagatat
    16561 aaagcgagcc aaagtcatta agtgattgtg aaatatttaa ctgcaaacgg tctttacggc
    16621 taaagcgtaa attgtggtaa ttcacgatta tcggttcatc gcgatgctca ccgtcgtaat
    16681 cgtaatcata ttcgtacccc tccattcgac gatacgcaac atcatcaagg gtataaaaac
    16741 cttgtgtcga atagcggtaa cccattaact gaaaattggt gccgaaggtg ttcatcgatt
    16801 tcgcatagag gaagcgaatg gaatccccct catgacgact gtcgtcggct aactgactgc
    16861 gcgcatgggt cacatccagc gacactgccc cccagttccc gaggttgcgc cccagcccta
    16921 ataaaaaggc ggtgtaattg gcagataatt gcgtcccgcc gtaggcagta aattcctgtg
    16981 gtaaaccgcc gagtgccgtg ccctgaaaaa agaaaggcga tgattgctga ctattaccgc
    17041 tacgaaaatc gcccgccgtc aggtcaaatt tgaaacgccc ttcgcgttgt aaaattggca
    17101 ctgttgaata cgggatagtg taattctgct gattaccatc gcgctcgtcg atggtgacgt
    17161 ccagatcgcc atttgaagat gtcgggtgca aatctgtaat ttcaaaagcg ccgggggaaa
    17221 catagctttg atagataata aaaccatttt gccgaatcgt tagctgggcc gccgtacggg
    17281 caatcccacg taccgttggg gcaaaccctt gctggctatc aggatacata ttatcggaag
    17341 aataaagccg tacaccacga aatccaacac catcgaaaat atcacttcct gtattgccgt
    17401 ctcccattac cagttcactt ttcagcggaa taatggcacg ctgtacccag gtgccaatat
    17461 tattccactg ttctgaatga tatccatttc cgcgaaaata gttccaggaa ccattgttgc
    17521 gtaaacgcca cgggccaata ttaatcccgc tgaactcact aaaaaaatag ctatcgttac
    17581 cattacctct gttaccggtg aaattataat tcaggagtaa agcaggaata ccttcatccc
    17641 actcttcagg cggaatgtaa ccgtgagcgc tactcaacaa cgctatctga ggaatagtga
    17701 tgttcaggcg catcgctgca aaatcaaagt taattgtggc atcaggtaca gctttcagta
    17761 aattgatgca tttattgttt tgctgctggg ctaactcggg aaatgcactg ctattaaggc
    17821 caattcgttc aaggagtacc tggttaaaac agggcattaa cccacctgat ttatcacctg
    17881 tattttctgt tgtcgattca aatacgatat cctgtgaacc aatgaactca tcattacgcc
    17941 agagatccac ccgatacacg cgcgcaggtt gatgattacc tctctcaaag cgcgataaat
    18001 cagcaacgga tgtgccattc tctaataaaa atgccgggtt gaaataactt tccgcatagc
    18061 ttaaaaagga gggtaaaatg gcaagagatg aataacaaat tccggccaga caccaggtat
    18121 acctctgcgg aatatccgta gtagtgggta ttttcataat tagctctgat attgtcagat
    18181 gattcggtta gtttaagttc acttctctga ccggggttac tgaaccataa tcattaacgg
    18241 tctgaaattt cagctttccc tgcactcctg agggtaaggg aatttgtgaa ctaactctgg
    18301 gtgccgccat agcattcccc aacttttggc tgcccacttg caggttaacg agggtgacat
    18361 aaaaaggtga tggattatga acattgatat agttaccgga tcgcgaaaac ttgagtgtgt
    18421 ccggcgcttc tgcgggtaat tcttgtaatt gaattggacg gagaaataat ttcatgcgcg
    18481 ataaaatcgc cagttgcaaa acattcctgc cgttcaatgc atttttatct accgaaggga
    18541 tcgtcttaac attcatccag aacagagact cacgatctgc cgccagcggt ggaccggtgt
    18601 aaataatacg caaggtattt tcgcttttgg gttcactaac aaacagtggc ggtgtaatga
    18661 tgaatgactt ttcttttaca ccgctgctgt tttcaatcca cgaattgacc agaaagcgct
    18721 cattggtatg gctatttcta atccataccg cagtctgttt agcatcagcg ggataaataa
    18781 tacgcgtggc acctaatgcg atccctccgg cagcatgggc gctggccgaa atgattaaat
    18841 aaaatataat gagcatcaat aactttattt tagtcatcat agttaatacg ccttgtgtat
    18901 tcctgacggc aaaaataagg aatataaaga aatgattgct ttggtttctt acaacacatc
    18961 attccctttt catttaataa agcggcatga tacaaagtga acatgatttc gatttacatt
    19021 gccaccatac cgcttcagac aaccggtaca ttactttgtt ataagacgtg atatataatt
    19081 caaaacaacg tggttttgat tattcatatc tcataataaa agtcgcgtca gcatttgctt
    19141 gccctgctga tgcacttgta ccggtgcctt tataacgtgc agaaaaatga agaacgttgg
    19201 tcccggggat caagttctgg ttggttgaga agctattccc atcaggtttg agaattgcgg
    19261 atgtattatc aagtatttca ataccgacac ccgttgctgt tgttgtattg gtactggatg
    19321 caatggccaa taaattatca ttgattgcat cagcctgacc agaaaatgca acggcagcat
    19381 tagcggcaac aacaggatcg cagtcgttca actgaatggt gaatggaatt aatgcagagg
    19441 tattaccaac agcattgaaa atatcggtac gatattgacc gagtgtgaca acctggtctg
    19501 ccgagtcagt attcactgaa caggcagcat tcaccagttc gccttcaaag tgaattgtac
    19561 cgccatctac aacggcagca taactacccg tagcggcgaa tagtgcggca gccagcgcag
    19621 acgaaataaa tcttaatttc atatatattc cttcaatctc atttatcgac tccacatccg
    19681 tatataaccg attactttat ttaagacact gatagtagta aattcctttt tatcctctaa
    19741 gaatgtctta attgaaaata tgcactctat tctaaaaaat agagagcccc gttagatgaa
    19801 tacttccgcg cataatatat tcaacacaaa tatagacctg aagcggtaaa ttacca
//
LOCUS       DAJOWB010000085        19634 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJOWB010000085 DAJOWB010000000
VERSION     DAJOWB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.085"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(465..841)
                     /gene="rnpB"
                     /locus_tag="OHO85_003267"
     ncRNA           complement(465..841)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OHO85_003267"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(874..2019)
                     /gene="garK"
                     /locus_tag="OHO85_003268"
     CDS             complement(874..2019)
                     /gene="garK"
                     /locus_tag="OHO85_003268"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCQ2672607.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2116..3006)
                     /gene="garR"
                     /locus_tag="OHO85_003269"
     CDS             complement(2116..3006)
                     /gene="garR"
                     /locus_tag="OHO85_003269"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCQ2672608.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3036..3806)
                     /gene="garL"
                     /locus_tag="OHO85_003270"
     CDS             complement(3036..3806)
                     /gene="garL"
                     /locus_tag="OHO85_003270"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCQ2672609.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     IDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     IEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3822..5156)
                     /gene="garP"
                     /locus_tag="OHO85_003271"
     CDS             complement(3822..5156)
                     /gene="garP"
                     /locus_tag="OHO85_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCQ2672610.1"
                     /translation="MILDTVDVKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWSVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5531..7102
                     /gene="garD"
                     /locus_tag="OHO85_003272"
     CDS             5531..7102
                     /gene="garD"
                     /locus_tag="OHO85_003272"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCQ2672611.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNIGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7251..7586
                     /gene="prlF"
                     /locus_tag="OHO85_003273"
     CDS             7251..7586
                     /gene="prlF"
                     /locus_tag="OHO85_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HCQ2672612.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7586..8050
                     /gene="yhaV"
                     /locus_tag="OHO85_003274"
     CDS             7586..8050
                     /gene="yhaV"
                     /locus_tag="OHO85_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HCQ2672613.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(8105..8914)
                     /gene="agaR"
                     /locus_tag="OHO85_003275"
     CDS             complement(8105..8914)
                     /gene="agaR"
                     /locus_tag="OHO85_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCQ2672614.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPTDSLEGLRKAGVEVILVGE"
     gene            9163..10443
                     /gene="kbaZ"
                     /locus_tag="OHO85_003276"
     CDS             9163..10443
                     /gene="kbaZ"
                     /locus_tag="OHO85_003276"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCQ2672615.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10466..10939
                     /gene="agaV"
                     /locus_tag="OHO85_003277"
     CDS             10466..10939
                     /gene="agaV"
                     /locus_tag="OHO85_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCQ2672616.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10950..11729
                     /locus_tag="OHO85_003278"
     CDS             10950..11729
                     /locus_tag="OHO85_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCQ2672617.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11719..12597
                     /locus_tag="OHO85_003279"
     CDS             11719..12597
                     /locus_tag="OHO85_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCQ2672618.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12615..13049
                     /locus_tag="OHO85_003280"
     CDS             12615..13049
                     /locus_tag="OHO85_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCQ2672619.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            13046..14179
                     /gene="nagA"
                     /locus_tag="OHO85_003281"
     CDS             13046..14179
                     /gene="nagA"
                     /locus_tag="OHO85_003281"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCQ2672620.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLCIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEKVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            14530..15684
                     /locus_tag="OHO85_003282"
     CDS             14530..15684
                     /locus_tag="OHO85_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HCQ2672621.1"
                     /translation="MPKNYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FSYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDDET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESSDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15697..16557
                     /gene="kbaY"
                     /locus_tag="OHO85_003283"
     CDS             15697..16557
                     /gene="kbaY"
                     /locus_tag="OHO85_003283"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HCQ2672622.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16724..17200
                     /gene="agaB"
                     /locus_tag="OHO85_003284"
     CDS             16724..17200
                     /gene="agaB"
                     /locus_tag="OHO85_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCQ2672623.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            17239..18042
                     /gene="agaC"
                     /locus_tag="OHO85_003285"
     CDS             17239..18042
                     /gene="agaC"
                     /locus_tag="OHO85_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCQ2672624.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            18032..18823
                     /gene="agaD"
                     /locus_tag="OHO85_003286"
     CDS             18032..18823
                     /gene="agaD"
                     /locus_tag="OHO85_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCQ2672625.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18824..19579
                     /locus_tag="OHO85_003287"
     CDS             18824..19579
                     /locus_tag="OHO85_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCQ2672626.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTAGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT         4745 a         5118 c         5009 g         4762 t
ORIGIN      
        1 ttcatgcttc ttaccctgtg caccaaaagc aaaaacttat cgatgcgggt ttgaaactga
       61 tggcaaccgc aaaaatgcct gatgcgctac gcttatcagg cctacgccat ctctgcaata
      121 tattgaattt gcgtgctttt gtaggcagga taaggcgttc acgccgcatc cggcatgaat
      181 aaagcgcact caacaatctg aaacccgccg gaacggttta ttacgtacat caggtgaaac
      241 tgaccgataa gccgctttct tttgggtata gcgtcgtgga cagtcattca tctttctgcc
      301 cctccaaaag caaaaacccg ccggtttacg ctggcgggtt ttcgttaaat caggtgaaac
      361 tgaccgataa gccgctttct tttgggtata gtgtcgtgga cagtcattca tttttctgcc
      421 cctccaaaag caaaaacccg ccgaagcggg tttttacgta aatcaggtga aactgaccga
      481 taagccgggt tctgtcgtgg acagtcattc atctaggcca gcaatcgctc actggctcaa
      541 gcagcctacc cgggttcagt acgggccgta ccttatgaac ccctatttgg ccttgctccg
      601 ggtggagttt accgtgccac ggactgttac cagccgcgcg gtgcgctctt accgcaccct
      661 ttcaccctta cctgatcccg cttgcgcggg ccatcggcgg tttgctctct gttgcactgg
      721 tcgtgggttt cccccccagg cgttacctgg caccctgccc tatggagccc ggactttcct
      781 cccctccgcc cgtctccccc gaagaggacg acgacgaagc ggcgactgtc tggtcagctt
      841 cggcgcgcag tatagagggt ttgcgcgccc ttgtcacccc gcgttgcgca ttccaatcgc
      901 cagtgtcgcg gcgatattac gtgaagcacg gcagatattg tcataagccc cgcggaatgc
      961 ttcgtccaac gtacctatgc tggtcaatac gctgaagacc gcatcaatgc catgctgatg
     1021 tacaacgcca acatcatcgg tcaggctacc cgcaatgcca atcaccggtt tatggtactt
     1081 cttcgccacg tttgcgacac caatcggtac cttcccgtga atactctggc tgtcaatacg
     1141 cccttcaccg gtgatcacca gcgtacaatc gtgaatatgt tcctccagat tcagcgccgt
     1201 agtgacgatt tcaataccac ttttcagttc cgcaccaaga aacgccatta gcgccgcgcc
     1261 cataccaccc gcagctcctg caccggggac atctttcaca tcaacatgca gcgctttttt
     1321 aatgacctcg gcatagtgag agaggttatt gtccagctca acaatcatcg cttcactggc
     1381 tcccttttgt gggccaaaga tgcgcgatgc gccgttatcg cccaccagcg gattggtgac
     1441 atcacaagcg acgcgaatga cgcaatcttt taagcgcgga tcgaggccgg aaatatcaat
     1501 atcattcaga gtattaagac taccgccgcc aaaaccaatt tcattgccgt tggcgtcgca
     1561 taatttcgcc cccagcgcct gtaccatgcc tgcgccgcca tcatttgtag cgctaccgcc
     1621 aatgccgata ataatgtttg tcgcgccgct ctccagcgcc tgcaggatta actcgcctgt
     1681 gccgcgtgaa gtggtcacga gtggatcgcg tttttccgca ggtaccagct ccagcccact
     1741 ggccgccgcc atttcaataa acgcggtttt gccatcgccg gagatccccc aactggcatt
     1801 cactttctcg cccagcggcc ctgtaaccca ggcgtgacgt tcagaaccct gggtggctgc
     1861 aatcatcgct tccaccgttc cttcgccacc gtcggcaacc ggaatagaaa cgtactgtgc
     1921 atcaggaaaa atttcccgaa atcctttttc tatcgcctgc gcaacctcgc tggcagataa
     1981 actttcttta taagagtctg gggcgattac gattttcata cctatgcctg ttaccacatg
     2041 acgccggagg gcgtttctct tattcggcct ggattccagg cccggattgc aatacgccat
     2101 ccgggcacga cgtcattaac gagtaacttc gactttcgcc agtttttcgt agtagcacgc
     2161 cagggcgcta tgatccgccg ttcctaaacc atctgctcgc agtgcctgca tcatctccat
     2221 aaccgcagct gtgagcggca gttgcgcgcc gacgccgtgg gaagtatcca gcgcatttgc
     2281 cagatcctta atatgcagat caatacggaa gcccggcttg aagttgcggt ccatcaccat
     2341 cggcgctttg gcatccagca cggtactgcc cgccagtcca ccgcgaattg cctgataaac
     2401 caggtccggg ttaacgcccg ctttggttgc cagcgttagc gcttctgaca tcgcggcaat
     2461 attcagcgcc acaatgacct gatttgccag tttggtgacg ttacctgcac cgatttcccc
     2521 ggtatgcacc acggaacccg ccatcgcttt catcaaatca tagtatttgt cgaaaatagc
     2581 cttgtcgccg cccaccatca ctgacagcgt accgtcgatg gctttcggtt caccgccgct
     2641 caccggagca tccagcatat caatgccttt cgctttcagc gcttcgctga tttcacggct
     2701 tgccagcggt gcgatagaac tcatatcgat caataccgta cctggcttcg cgccttcaat
     2761 gatgccattc tcacccagcg ccacctcttt cacatgaggg gagtttggca gcatggttat
     2821 gatgacgtcg cactgttcag cgatcgcttt agccgtagac gctgtttctg cacctgcagc
     2881 aatcacgtca gcaatagctt ctgggttacg gtcagcaacc accagcgagt aacctgcttt
     2941 cagaaggttt ttactcattg gtttacccat aatccccagg ccaataaaac caactttcat
     3001 agtcatatca atcatctctc ttgttgcggt ggtggttatt ttttaaaggt atcagccagt
     3061 ttctgagtgg cagagcggaa gacgccgaga tcgctgccga cagccacaaa cgtcgcgccc
     3121 cattccagat aacgacgcgc atcggcttcg atcggtgcga ggataccgct gggtttgccg
     3181 tgcgcactgg cacggttaaa aatgtgctga attgcttttt gtacatccgg gtgcgaggca
     3241 ttgccgagat ggcctaatgc cgcagccaga tcgctggggc cgacgaagat gccgtctacg
     3301 ccttcggtag cggcaatggc atcgatgtta tctacgccct gctgactttc tatctggacc
     3361 agaatagtga tgttcttgtt cgactgagcg aaataatccg ccacggtgcc aaacatattg
     3421 gcgcggtgag aaacggagac gccgcgaatg ccttccggtg ggtaacgggt tgatgccacc
     3481 gcctgctctg cttcctcttt tgtttctaca aaaggaatca ggaagttata gaaaccgata
     3541 tccagaagac gcttaataat taccggctcg ttggtcggca ctcgcactac tggcgcgctg
     3601 gcgctgcctt tcagggccat taactgcgga ataaacgtgg aaatatcgtt tggcgcatgt
     3661 tcgccatcca gcaccagcca gtcaaaccca gccaaaccaa gaacttcagt gctaatcggg
     3721 ttagagagtg ctgaccagca accaatttgt acctgtttcg cagccagtgc ggctttgaat
     3781 ttattcggga aaacatcgtt attcatcgct tatacctttg cttatttctg caattccata
     3841 cgtttaatgt cgccaactac gaagaggtag cagaccatcg ccatcagcgc tgaacatccc
     3901 acgaaaacca gtgctgcatt gaaggagtgc agttcactta ccaggtagcc aatcaccagt
     3961 ggagtgacaa tggaggcaac attgccaaag acgttaaaga cgccgccgca gaggccaaca
     4021 atctctttcg gcgcggtgtc agaaatcacc gaccagccca gagcaccaaa tcctttgcca
     4081 aaaaaagcca gcgccatcag catgaccacc agcgtggtgt tgttggtgta gttacataag
     4141 atgatggtgg aagccagcaa cattcccagc acaatcggta gcttacgtgc cagggtcagg
     4201 gataaaccgc gtttgatcag ataatccgag aagacacctc ccagcacgcc gcccgcaaaa
     4261 ccacacagtg ccggaatcga ggcgaccaga cccactttca gaatcgacat gcctttttcc
     4321 tgcaccagat aaatcgggaa ccaggtgagg aagaaccagg tgatggtgtt gataaaatat
     4381 tgtccgaaaa atacgcccaa catcatgcgg ttagagagca attgcttgat gtaatgcagt
     4441 ttgggaccgc ttgctgccgc gctgcccggc tttttgtggt ccatatcgac caccgcgcca
     4501 ttttcagaga taaacttcag ctcttccgca gacatacgtg ggtgatctgt cgggttatga
     4561 atcaacttga tccacagcgc cgtcagcaca aaaccaatca cccccataac ggtgaagacg
     4621 tgctcccagc cccaggcgaa agtcagccag ccaagcagcg gcgaaaagag cgccagcgag
     4681 aaatattgcg ccgagttaaa gatggcggag gcagtaccac gttctttcgt cgggaaccag
     4741 gcggcgacaa tacgggcgtt cgccgggaat gatggcgctt ccgagaaccc cagcataaag
     4801 cgcataaaga acatggagat ccctgcccag gccagcggga acatatcaac aaagccttgc
     4861 aggaaggtga acagcgacca gaaaaagagg ctgtaggtgt aaactttttt cgagccaaac
     4921 ttatcaagca gccagccgcc gggaatttgc atcagcaggt aggcccagcc aaaagcggag
     4981 aagatgtaac ccatcgaaac cgcacttaac tgcaactctt ttgccacttc ggtaccagca
     5041 atagacagcg ttgcacgatc ggcgtagtta acggcggtaa caataaaaat aatcagtaat
     5101 attaaatagc gggtatgcac gcctttcttt tttacgtcaa cggtgtccag aatcatttta
     5161 tttacctcgg gtacttatgc tgatttttat tattatggtg aaggtgttat ttatgagttt
     5221 tatttatgcc gttacgacaa tgaactcggg aattagtata agcagcgcga gaataataat
     5281 cattgtgcaa atgctaattt aattaatacc atttaaatat tattttgagc atatgcacat
     5341 aaggttgcgc gctaaagcac agatttgcgc tttaccttgc cgggcggcac tgcaatccct
     5401 gaaatgattg acattgatca catttctgcg tttaaactcc tgacattctt atttcaccca
     5461 atgaagtcat ttatttttaa atgagaccag gtcctcattt taataacccc tggctggaga
     5521 atattgcaca atggccaaca tcgaaatcag acaagaaacg ccaactgcgt tttatataaa
     5581 agttcacgac acagataatg tggcaattat tgttaatgat aatggcctga aagcaggaac
     5641 gcgttttccg gatgggctgg aattaattga acatattccc caggggcata aagtcgcatt
     5701 gctggacatt ccggctaatg gtgaaattat tcgttatggc gaagtgattg gttacgccgt
     5761 gcgcgcaatc ccacgcggaa gctggatcga cgaatcaatg gttgtactac cggaagcgcc
     5821 gccgttacac acgctgccac tggcaaccaa agtcccggaa cccttaccgc cgctggaagg
     5881 atacaccttt gagggctatc gcaatgccga tggcagcgtg ggcaccaaaa acctgctcgg
     5941 catcaccacc agcgtccact gtgtggcagg cgtggtggac tacgtagtaa aaatcattga
     6001 acgcgatctg ctaccgaaat acccgaacgt cgatggcgtg gtggggctga atcatttgta
     6061 cggttgtggc gtggcgatta acgcaccggc ggcagttgtg cctattcgta ccattcacaa
     6121 tatttcgctg aaccctaact ttggcggcga agtaatggtg attggcctgg gttgtgaaaa
     6181 gttgcagcct gagcgcctgc tgactggaac ggatgatgtg caagctattc cagtagaaag
     6241 cgccagcatt gtcagtttgc aggatgaaaa gcatgtcggt tttcagtcca tggtcgagga
     6301 tattttgcag gtcgccgaac gccatctaca aaaactgaat caacgtcagc gagaaacctg
     6361 tccggcttca gaactggttg tcggcatgca gtgcggtggc agcgatgcct tttctggcgt
     6421 aacggcaaac ccggcggttg ggtatgcgtc tgatctactg gtgcgctgcg gcgcaacggt
     6481 gatgttctca gaagtcacgg aagtgcgtga cgcgatccat ctgctgacac cacgcgcagt
     6541 gaacgaagag gtcggcaaac ggctgctgga ggagatggag tggtacgata actatctcaa
     6601 tataggaaaa accgaccgca gcgccaaccc ttcgccgggc aacaagaaag gcggtctggc
     6661 aaacgtggta gagaaggcac tcggctccat tgctaaatcg ggtaaaagcg caattgttga
     6721 agtgctgtcg cccggtcaac gcccgactaa acgcggatta atttacgccg cgacgccagc
     6781 cagcgatttt gtctgtggca cgcaacaggt ggcttcgggt atcaccgtgc aagtgtttac
     6841 gaccggtcgt ggtacgccgt acggcctgat ggcggtaccc gtcattaaaa tggcgacccg
     6901 caccgagctg gcgaaccgct ggtttgattt aatggatatt aacgcgggca ctatcgccac
     6961 cggcgaagaa accattgaag aggtgggctg gaagttgttc cactttattc tcgacgtcgc
     7021 cagcgggaag aagaaaacct tctcggatca atgggggctg cataaccagc tggcagtgtt
     7081 taacccggca ccggtgacct gatttctttt ggaacgtccc tcgcaaaaca tggccttagt
     7141 gccatgtttt tattgtttaa agcccccacg tccattaata atgcatttgc attaccttag
     7201 ttcaagctta taatttgagc agaaaacagg ctgtaaaagg acagtgaatc atgcccgcta
     7261 atgctcgctc tcacgctgta ctgaccactg aatcaaaggt cacgatacgc ggacaaacaa
     7321 ctatccccgc gccagtgcgt gaggccttaa aactgaagcc aggccaggac agcattcatt
     7381 acgaaattct gcctggtggg caagtcttta tgtgccgact gggagatgaa caggaggatc
     7441 atactatgaa tgcatttttg cgttttctgg atgcagatat ccagaacaac ccgcaaaaaa
     7501 ctcgtccatt caacattcaa caaggaaaga aacttgtcgc tggcatggac gtcaacattg
     7561 atgatgagat tggcgacgac gaataatgga ttttccacaa agggttaatg gttgggcgct
     7621 atatgctcat ccctgttttc aggaaaccta cgatgcttta gttgccgaag tcgaggcatt
     7681 aaagggaaaa gatcctgaaa attatcagag aaaagctgcc acaaagttat tggcggtggt
     7741 ccataaagtg attgaggagc atatcacggt caatccatca tcaccggcat tccgtcatgg
     7801 caagtcgtta ggctctggga aaaataaaga ctggtcacgg gtaaaatttg gtgctggtcg
     7861 ctatcgtctc ttctttcgtt acagtgaaaa agagaaagtc atcattctgg gatggatgaa
     7921 cgatgaaaac acgctgcgca cctacggtaa aaaaacagat gcctataccg tattcagtaa
     7981 aatgttaaaa agaggacatc ctcctgccga ctgggaaagc ctcacccaag agacagaaga
     8041 aaaccattga tggtgttcac aatgcccgcc gaacgtattc gtgttgacca gcgggcaccc
     8101 tttctcactc cccgaccaga atcacttcaa ccccagcctt tcgcagtcct tccagactat
     8161 ccgtaggaat gccttcatca acaatgatca tgtcgatacg ttgagtatca atgatcttat
     8221 gtaaactgga acgattgaac ttactggaat cggtgactac gatgatccgt tccgcaactt
     8281 cgcacatccg gcggtttaaa cgggcttcat cttcattatg cgtgctgacg ccgcgctcca
     8341 gatcgatcgc atctacacca agaaacagca tatcgaagtg gtaattttgc agcgattgtt
     8401 cagcctgatc gccgtaaaaa gattgcgact gacggcgcaa atgcccgccg gtcatcagca
     8461 gctcaacgcc ttccgcttcc agcaaagcat tagccacgtt cataccgttg gtcatcgcaa
     8521 ttacgtcagt gtgcttgcgc atcagacgag caatctcaaa agtggtagtc ccggaatcga
     8581 ggatcacccg atgacctggc tgaatcaact caacggcagc tttcgcaacg ctgcgtttca
     8641 tcgcggtgtt cagtgcgctt ttatcttcca ctgatggctc gactgacggc gtcgtgctat
     8701 cgcagatcaa cgcgccacca taggcacgca cagcgatccc ctgcttttcc agaaacgcca
     8761 gatcgttgcg gatcgtcacg gtagatacgc catacaatgc cgacagatcg ttaacctgca
     8821 cactcccttg ctgtcgcaga cgctgaatga tctgttctcg tcgctcgctg gtgcctgtca
     8881 ctcgcttctc acctgaagcg tcggtattac tcatagtaag tcctttcgta aaactttcgt
     8941 ttcatttcgt tttgcctatt aacgcctttc tattaagcaa atgcaagccc accttgccca
     9001 ttgacgcaag ctactctcgt ttcagtgact ttcattatgt ttcttttgtg aatcagatca
     9061 gaaaactatt atctttcgtt ttatttttat ctcaccatga cgcagtatca actgaaacaa
     9121 aacgaaagat taatatcgca gtaatctgaa ctggagagga aagtgaaaca tctaacagaa
     9181 atggtgagac agcacaaagc gggcaaaaca aatgcaattt atgccgtttg ttccgcacat
     9241 ccgctggtgc tggaagctgc aatccgctac gccagtgcaa accaaacgcc gttactgatt
     9301 gaagcaacct ccaatcaggt agaccagttc ggcggttata ccggaatgac gcccgccgat
     9361 tttcgcggct ttgtttgtca gctcgccgac tcgttgaatt tcccgcagga tgcgttgatt
     9421 ctgggtggtg accatctggg gccaaaccgc tggcaaaacc tgccagccgc tcaggcaatg
     9481 gccaatgccg atgatttgat taaaagctac gttgcggcag gattcaaaaa aatccacctt
     9541 gattgcagca tgtcctgtca ggacgatcca attcccttaa ctgatgacat cgtggctgaa
     9601 cgcgccgccc gtctggcgaa agtggcggaa gaaacctgtc ttgaacactt tggcgaagcc
     9661 gatctggagt atgtcattgg taccgaagtg ccggtacctg gcggcgcgca tgaaacctta
     9721 agcgagctgg cggtcaccac gccggatgcc gcccgcgcca cgctggaagc ccatcgtcac
     9781 gcctttgaaa agcaaggttt gaatgccatc tggccacgca tcattgccct ggtggttcaa
     9841 cccggcgtcg aattcgatca caccaacgtt attgattatc agcccgccaa agcgagcgcc
     9901 ttaagccaga tggtcgaaaa ctacgaaacg ctgattttcg aagcgcactc taccgattat
     9961 caaacgccgc aatcgctgcg ccagctggtg attgaccact ttgccattct gaaagttggc
    10021 ccagcgctga ccttcgccct gcgtgaagct ctgttctctc tggcggcgat tgaagaagaa
    10081 ctggtgccag cgaaagcctg ttctggtctg cgtcaggtgc tggaagacgt gatgctcgac
    10141 cgcccggaat actggcaaag ccactaccac ggtgacggca acgcgcgtcg tctggcgcgt
    10201 ggttatagct actcggatcg cgtgcgctat tactggccgg acagccagat tgatgacgct
    10261 ttcgctcatc tggtacgtaa tctggcggat tcaccaattc cgctgccgct gatcagccag
    10321 tatctgccgc tgcagtacgt gaaagttcgc tccggcgagc tgcagccaac gccacgggaa
    10381 ctcattatca accatattca ggacatcctg gcgcagtacc acacagcctg tgaaggccaa
    10441 taagcaaaac aaagaggaac acgctatgcc aaatattgtt ttaagccgga ttgatgaacg
    10501 cttgattcac ggtcaggtcg gcgttcaatg ggtcggattt gcgggggcaa atctggtgct
    10561 ggtagccaac gatgaggttg ccgaagatcc ggtacaacaa aacctgatgg aaatggtact
    10621 ggcagaaggg atcgccgtac gtttctggac gctgcaaaaa gttatcgaca acattcatcg
    10681 cgccgccgat cgacagaaaa tcctgctggt ttgtaaaaca cccgccgatt tcctgacgct
    10741 ggtgaaaggt ggcgttccgg tgaatcgcat taacgttggc aatatgcact acgccaatgg
    10801 caaacaacaa atcgccaaaa cggtttctgt ggatgcgggc gatatcgcag catttaacga
    10861 cctgaaagcc gctggggtgg aatgcttcgt tcagggcgtc ccgacagagc ctgctgtgga
    10921 cctctttaaa ttactttgag ggattcatca tggaaatcag cctgttgcag gcatttgcgt
    10981 tgggcattat cgcctttatc gctggcctgg atatgtttaa cggcctaaca catatgcacc
    11041 gcccggtggt cctcggcccg ttggtcgggc tggtacttgg cgatctgcat accggaattt
    11101 taaccggcgg tacgctggaa ctggtgtgga tggggctggc cccgctggcg ggcgcacagc
    11161 cgcctaacgt gattatcggt actatcgtcg gcacggcgtt tgccattact actggcgtga
    11221 aacccgatgt cgcagtcggt gtcgccgtac ctttcgctgt cgcagtacag atggggatta
    11281 ccttcctgtt ctcggtgatg tccggcgtga tgtctcgctg cgaccggatg gcggagaacg
    11341 ccgatactcg cggcattgaa cgcgtgaact atctggcact gctggcactc ggcactttct
    11401 attttctctg cgctttcctg cctatctact tcggcgcgga acatgcaaaa accatcattg
    11461 atgtcctgcc gcaacgatta atcgacggcc tcggcgtcgc gggcggcatc atgccagcaa
    11521 tcggttttgc cgtgctgctg aaaatcatga tgaaaaacgt ctacatcccc tacttcatcc
    11581 tgggctttgt tgccgcagcc tggctcaagt taccggtgct ggctattgct gccgctgcgc
    11641 tggcgatggc gctgatcgac ctgctgcgta aatctcctga accgactcaa cctgcggcac
    11701 agaaagagga atttgaagat ggcatctaat caaaccaccc tgccgaacgt cagtgaaaac
    11761 gaagaaacat tgctgactgg cgtcaatgaa aacgtgtatg aagatcagag cattggcgcg
    11821 gagctgacga aaaaagatat caaccgcgtc gcctggcgtt ccatgctgtt acaagcgtct
    11881 tttaactacg aacgtatgca ggcttctggc tggttgtacg gtctgctgcc tgctctgaaa
    11941 aagatccaca ctaataaacg cgacctggcg cgcgccatga aggggcatat gggtttcttc
    12001 aatacccatc cgtttctggt gacatttgtt atcggcatta tccttgcgat ggagcgttct
    12061 aagcaggacg ttaacagtat tcagagcacc aaaattgccg tcggtgcgcc gctcggcggg
    12121 attggcgatg caatgttctg gctaacgcta ctgccgattt gtggcgggat tggagccagt
    12181 ctggcattgc aaggctccat tcttggcgca gtcgtcttta ttgtgctgtt caacgtggta
    12241 cacctggggc tgcgttttgg tctggcgcat tatgcttacc gcatgggcgt ggcggcgatt
    12301 ccactcatta aagcgaatac caaaaaagtc ggccatgctg cgtcaatcgt tgggatgacg
    12361 gtaatcggcg cgctggtggc aacctatgtt cgtttgagca ccacgttgga aatcacagcg
    12421 ggcgacgcag tggttaagtt acaggctgat gttatcgaca aactgatgcc tgccttctta
    12481 ccgttggtct acaccctgac catgttttgg ttggtacgcc gcggctggag tccgctgcgt
    12541 ctgattgcag ttaccgtggt tctcggcatc gtcggtaaat tctgccattt cctttaaata
    12601 caatgaggta ttagatgtta agtattattt tgacagggca tggcggattt gccagcggca
    12661 tggaaaaagc aatgaagcaa atcctcggcg agcaatcgca gtttatcgcc atcgattttc
    12721 cggaaacctc aagcaccgcg ctgcttacat cgcagcttga agaggccatc gcccaactgg
    12781 attgtgaaga cggcattgtt tttctgacgg atttgctggg cggcacgccg tttcgcgtag
    12841 cttcaacgct ggcgatgcaa aaaccgggct gtgaagtgat caccggcacc aatttgcaac
    12901 tgctactgga gatggtgctg gagcgcgaag ggttaagcgg tgaagagttc cgtgtgcagg
    12961 cgctggagtg tggacaccgt gggctgacca gtctggtaga cgagttaggc cgctgccatg
    13021 aagaatgtcc ggtcgaggaa ggaatatgac acacgttctg cgcgccagaa ggctgctgac
    13081 tgaagaggga tggctcgatg accatcagtt gtgtattgct gacggtgtaa tcgcagcaat
    13141 cgaaccgatt ccagtgggcg tgactgaacg cgatgcggaa ctgctctgcc ccgcttatat
    13201 cgacacccat gtacacggtg gtgcgggcgt tgatgtaatg gatgacgcgc cggatgtact
    13261 cgacaagctg gcaatgcaca aggcacgcga aggtgtcggc agttggttgc cgactaccgt
    13321 aaccgcgccg cttaacacca ttcatgcggc gctgaaacgt attgctcaac gttgccaacg
    13381 cggcggacct ggtgcgcaag tgctggggag ttatctcgaa ggaccgtact tcacgccgca
    13441 gaataaaggc gcgcatccgc cggagctgtt ccgcgagctg gaaattgccg agctggatca
    13501 gttgattgcc gtttctcagc acaccttacg tgtggtagcg ctggcaccgg aaaaagaagg
    13561 ggcattgcag gccatccgcc atcttaaaca gcaaaacgta agggtgatgc tggggcatag
    13621 cgcggcgacc tggcaacaaa cccgcgccgc atttgatgct ggtgccgacg gcctggtgca
    13681 ttgctataac gggatgacag gtttacatca ccgcgaaccg ggaatggttg gcgcgggatt
    13741 aacggacaag cgcgcctggc tggaactgat agccgatggt catcatgtgc atccggcggc
    13801 gatgtcgctg tgttgttgct gtgcgaaaga gagaatcgtg ctgatcaccg acgcgatgca
    13861 ggcagccggg atgccggatg gtcgctatac gttatgtggt gaaaaagtgc agatgcacgg
    13921 tggcgttgtc cgtaccgcgt ctggtgggct ggcgggcagt acgctgtctg ttgatgcggc
    13981 agtgcgcaac atggtcgagt tgacgggcgt aacgcctgcg gaagccattc atatggcatc
    14041 gctgcatccg gcgcgaatgc tgggtgttga tggtgttctg ggatcgctta aaccgggcaa
    14101 acgcgccagc atcgttgcgc tggatagcgg gctgcatgtg caacaaatct ggattcagag
    14161 tcaattagct tcgttttgat agtttgctcc tttattgggc cttcacttcc cccgtaaggc
    14221 ctttcttttt ctttcgtttt gatctgtgca gcggtgtcgg atgcgacgct aacgcgtctt
    14281 atgcgaccta cggtacgccg catccagcaa tcggtgaaca tagcgacgtc attacgcaat
    14341 tctttaatct tcccttgtct tttctttcaa tgatcacaga tttcgtttta tttccttttt
    14401 ctccattgaa ctttcggttt cttttctata gattttaatc aacgaaagac atcaccaagt
    14461 gaaatgaaac gaaaggcaag tgaaagcgac aacgcccgac gtcaagttca tcagactaag
    14521 gattgagtta tgccaaaaaa ttacacccct gctgccgccg caaccggtac atggactgaa
    14581 gaagagatcc gccatcagcc tcgcgcatgg atccgttcac tcaccaacat cgacgcgcta
    14641 cgttccgcgc tcaataactt ccttgaaccg ttactgcgca aagagaatct gcggatcatc
    14701 ctgaccggag ccggaacctc ggcatttatc ggtgacatca tcgcgccgtg gctcgccagc
    14761 cataccggta aaaacttcag cgccgtaccg accaccgatc tggtcactaa tccgatggac
    14821 tacctgaacc cagcccatcc gctgctgttg atctccttcg gtcgatccgg caacagcccg
    14881 gaaagcgtcg cagccgtgga actggcaaat caatttgtac cggaatgcta tcacctgccg
    14941 atcacctgca acgaagcggg cgctctttac caaaacgcga tcaacagcga taacgcgttt
    15001 gccctgctga tgcccgcaga aacgcacgat cgcggctttg cgatgaccag cagcattacc
    15061 accatgatgg ccagctgcct cgcggttttc gcacctgaga cgatcaacag ccaaaccttc
    15121 cgcgacgtgg cggatcgttg ccaggcgatc ctgacctcac tgggcgattt cagcgaaggt
    15181 gtgtttagtt acgcaccgtg gaaacggatc gtttatctcg gtagcggtgg cttacagggc
    15241 gcagcacgcg agtcggcgct gaaagtgctg gaactgacgg cgggtaaact ggcggccttt
    15301 tatgattctc caaccggatt ccgtcatgga ccaaaatcgc tggtcgatga cgaaacgctg
    15361 gtggtggtat ttgtctccag ccacccttac acccgtcagt atgatcttga tctgctggct
    15421 gaacttcgcc gtgacaacca ggcaatgcgt gtaatcgcca tcgccgcgga aagcagcgac
    15481 atcgtcgctg ccggtccaca tatcatcctg ccaccgtcac gtcactttat cgacgttgag
    15541 caggcatttt gcttcctgat gtacgcccag acgtttgcac tgatgcagtc gctgcacatg
    15601 ggcaatacgc cggatacccc atcagccagt ggcaccgtta accgcgtggt gcaaggcgta
    15661 atcattcatc cgtggcaggc ataagaggat cgcattatga gcattatctc cactaaatat
    15721 ctgttacagg acgcccaggc caatggctac gcggtgcctg cttttaacat tcataacgcc
    15781 gagacgatcc aagcgatcct cgaagtgtgc agtgaaatgc gatcgccggt gatcctcgcc
    15841 ggaacgccgg ggacctttaa acacatcgcg ctggaagaga tctacgccct gtgtagcgcc
    15901 tattccacaa cctacaacat gccactggcg ctgcatctcg accaccacga atcgctggat
    15961 gatattcgcc gtaaagtcca cgcaggcgtg cgcagtgcga tgatcgacgg cagccacttc
    16021 ccgtttgccg agaacgtaaa gctggtgaaa tcggttgtcg acttctgcca ctcccaggat
    16081 tgcagcgtgg aagcagaact gggccgcctg ggcggtgttg aagatgacat gagcgttgac
    16141 gccgaaagtg cattcctgac cgatccacaa gaagctaaac gctttgtcga actgactggc
    16201 gtcgacagcc tggcggtagc gattggtacg gcgcacggct tatacagcaa aacgccgaag
    16261 attgatttcc agcggctggc ggaaattcgt gaagtggtgg atgttcctct ggtgctgcat
    16321 ggtgccagcg atgttccgga tgaatttgtc cgtcgcacta ttgaacttgg cgtcacaaaa
    16381 gtgaacgttg ccacagaatt aaaaatagcc ttcgctggcg cggttaaagc ctggtttgcg
    16441 gaaaatccgc agggtaatga tcctcgttat tatatgcgcg tcggaatgga tgcgatgaaa
    16501 gaagttgtca gaaataaaat taatgtctgt ggttcagcga atcgaatttc agcataatca
    16561 ttagtttttt atgatttatc ccaatgtact tcccgattaa tatcggggag tgccttaatg
    16621 gaaaaggaga taactaaacc ttaataaata catcagtaca atatcgcaac aataatatat
    16681 ttaaaaaaat tatattattc aactttatgg tgaggattac acaatgagca gtccaaatat
    16741 tctcttaacc cgtattgata accgtctggt tcatggccag gttggcgtga cctggacatc
    16801 caccatcggt gcaaatctgc tggtagtcgt ggatgatgtt gtcgctaacg atgatattca
    16861 acagaaatta atgggtatta ccgcggaaac ctacggcttt ggcattcgtt tctttactat
    16921 cgaaaaaacc attaacgtca tcggcaaagc tgcaccacat cagaagatct tcctgatttg
    16981 ccgtacgcca caaacggtac gtaaattggt agaaggtggc attgacctga aagatgtcaa
    17041 cgtcggcaat atgcatttct cggaagggaa aaagcaaatc agcagtaaag tttatgtcga
    17101 tgaccaggat ctcacggact tacgttttat taaacaacgt ggcgtgaatg ttttcattca
    17161 ggatgtccct ggcgatcaaa aagaacaaat ccctgactaa atctaaaatc gccttaatat
    17221 tggtttgagg taataaaaat gcatgaaata accctacttc agggattatc cctggcggcg
    17281 ttagttttcg ttctggggat tgatttttgg ctggaagcct tatttttatt ccgcccgata
    17341 atcgtttgta cccttacagg tgctattctc ggtgatattc agactggctt aattaccggt
    17401 ggtctgacag agttggcttt cgccggatta acccctgcag gtggtgttca gccgcccaac
    17461 ccgattatgg cgggtctgat gaccaccgtc attgcatggt ctacgggcgt tgatgccaaa
    17521 acagcaattg gtcttggcct gccgtttagt ttgttaatgc agtacgtcat tctgttcttc
    17581 tattccgctt tctcattatt tatgaccaaa gccgataaat gcgcgaaaga ggcggatacg
    17641 gcagcgtttt cccggcttaa ctggacaacg atgctcatcg tcgcttcagc gtatgcggtg
    17701 attgctttcc tctgtactta cctggcacaa ggggcgatgc aggcgctggt gaaagcgatg
    17761 cccgcctggc tgacccacgg ctttgaagtg gctggcggta ttctgcctgc cgttggtttt
    17821 ggcttgctgc tgcgcgtgat gttcaaagca caatatatcc cttacctgat cgccggcttc
    17881 ctgtttgttt gctacatcca ggtcagtaac ctgttgccgg ttgccgtact gggcgcaggc
    17941 tttgcggtgt atgagttttt caatgcgaaa tcccggcagc aagcgcaacc gcagcccgtt
    18001 gccagtaaaa atgaagaaga ggactacagc aatgggatct gaaatcagta aaaaagatat
    18061 cacccgtctg ggctttcgtt cgtcactgct gcaagcgagc tttaactacg aaaggatgca
    18121 ggcgggcggt tttacctggg cgatgttgcc gatcctgaaa aagatttata aggatgacaa
    18181 accgggccta agcgcagcga tgaaagataa cctcgaattt attaataccc acccgaatct
    18241 ggtcggattc ctgatggggt tattaatttc gatggaagaa aaaggagaaa accgcgacac
    18301 cattaaaggc ctcaaagtgg cactgtttgg cccaatcgcc gggattggcg atgcgatttt
    18361 ctggtttact ttgttgccga ttatggcggg aatttgctca tcatttgcca gccagggaaa
    18421 cctgctgggg ccgattctat ttttcgccgt ttacctgctt atctttttcc tgcgcgtcgg
    18481 ctggacccac gtcggttatt cagtcggcgt gaaggcgatc gataaagtac gagagaactc
    18541 gcagatgatt gcccgttcgg caaccatcct cgggatcacg gtaatcggcg ggctgatcgc
    18601 ttcgtatgtg catattaacg tggtgacatc gtttgccatc gacagtaccc acagcgtcgc
    18661 gctgcagcag gatttcttcg ataaagtctt cccgaacatt ttaccgatgg cctacaccct
    18721 gctgatgtat tacttcctgc gggtgaaaaa agcgcatccg gtgctgttaa tcggcgtgac
    18781 ttttgtgctc tctattgttt gttccgcatt cggcattttg taaatggaac gaggcactgc
    18841 gtctggtggt gcctctttac ttaaggaatt tcatcctgtg caaacccttc agcaagttga
    18901 aaactatacg gcgttaagtg aacgtgccag cgaatattta ttggctgtga tccgtagcaa
    18961 accggatgcc gtgatttgcc tggcgactgg agccacgcca ttactgacgt atcattatct
    19021 ggtagaaaaa atccatcagc agcaggttga tgtcagccaa ctcaccttcg tgaagctcga
    19081 cgaatgggtg gatctgccat taacgatgcc tggcacctgc gaaactttcc tgcaacagca
    19141 tatcgtgcag ccgctggggc tacgtgaaga ccagctcatc agctttcgct ccgaagagat
    19201 aaatgagaca gagtgcgaac gggtaacgaa cctgattgcg cgcaaaggcg gtctggattt
    19261 atgcgttctc ggattgggga aaaacggtca tcttgggctg aacgaaccgg gagaaagcct
    19321 gcaaccggcc tgccatatca gtcaacttga tgccagaaca cagcaacatg agatgttaaa
    19381 aaccgcgggt cgccccgtga ctcgtgggat caccttaggc ctgaaggata ttctcaatgc
    19441 ccgcgaagtt ttgttactgg tgactggcga aggaaagcag gatgcgacag aacgttttct
    19501 cacggctaaa gtctccaccg ctatcccggc ttcgttttta tggctgcaca gtaactttat
    19561 ttgtttaatt aacacctaac cagatgaaaa atttgctcaa aatgtttaaa ttaacttatg
    19621 taacagtcat gcat
//
LOCUS       DAJOWB010000086        19547 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJOWB010000086 DAJOWB010000000
VERSION     DAJOWB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.086"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(40..2715)
                     /gene="adhE"
                     /locus_tag="OHO85_003288"
                     /gene_synonym="adhC"
     CDS             complement(40..2715)
                     /gene="adhE"
                     /locus_tag="OHO85_003288"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCQ2672627.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            3192..3839
                     /gene="ychE"
                     /locus_tag="OHO85_003289"
     CDS             3192..3839
                     /gene="ychE"
                     /locus_tag="OHO85_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCQ2672628.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(3996..4292)
                     /locus_tag="OHO85_003290"
     CDS             complement(3996..4292)
                     /locus_tag="OHO85_003290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672629.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            4576..6207
                     /gene="oppA"
                     /locus_tag="OHO85_003291"
     CDS             4576..6207
                     /gene="oppA"
                     /locus_tag="OHO85_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCQ2672630.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            6293..7213
                     /gene="oppB"
                     /locus_tag="OHO85_003292"
     CDS             6293..7213
                     /gene="oppB"
                     /locus_tag="OHO85_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCQ2672631.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            7228..8136
                     /gene="oppC"
                     /locus_tag="OHO85_003293"
     CDS             7228..8136
                     /gene="oppC"
                     /locus_tag="OHO85_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCQ2672632.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            8148..9161
                     /gene="oppD"
                     /locus_tag="OHO85_003294"
     CDS             8148..9161
                     /gene="oppD"
                     /locus_tag="OHO85_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCQ2672633.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTLGRLRACFKPVE
                     ELL"
     gene            9158..10162
                     /gene="oppF"
                     /locus_tag="OHO85_003295"
     CDS             9158..10162
                     /gene="oppF"
                     /locus_tag="OHO85_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCQ2672634.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10215..10544)
                     /locus_tag="OHO85_003296"
     CDS             complement(10215..10544)
                     /locus_tag="OHO85_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCQ2672635.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(10579..12039)
                     /gene="cls"
                     /locus_tag="OHO85_003297"
     CDS             complement(10579..12039)
                     /gene="cls"
                     /locus_tag="OHO85_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCQ2672636.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            12182..12355
                     /locus_tag="OHO85_003298"
     CDS             12182..12355
                     /locus_tag="OHO85_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCQ2672637.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(12410..13663)
                     /gene="kch"
                     /locus_tag="OHO85_003299"
     CDS             complement(12410..13663)
                     /gene="kch"
                     /locus_tag="OHO85_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCQ2672638.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(13963..14259)
                     /locus_tag="OHO85_003300"
     CDS             complement(13963..14259)
                     /locus_tag="OHO85_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCQ2672639.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            14483..15202
                     /gene="tonB"
                     /locus_tag="OHO85_003301"
     CDS             14483..15202
                     /gene="tonB"
                     /locus_tag="OHO85_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCQ2672640.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPAVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(15242..15640)
                     /gene="yciA"
                     /locus_tag="OHO85_003302"
     CDS             complement(15242..15640)
                     /gene="yciA"
                     /locus_tag="OHO85_003302"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCQ2672641.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(15745..16284)
                     /locus_tag="OHO85_003303"
     CDS             complement(15745..16284)
                     /locus_tag="OHO85_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCQ2672642.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(16314..17057)
                     /locus_tag="OHO85_003304"
     CDS             complement(16314..17057)
                     /locus_tag="OHO85_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCQ2672643.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(17085..17174)
                     /locus_tag="OHO85_003305"
                     /pseudo
     CDS             complement(17085..17174)
                     /locus_tag="OHO85_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            17414..18052
                     /gene="ompW"
                     /locus_tag="OHO85_003306"
     CDS             17414..18052
                     /gene="ompW"
                     /locus_tag="OHO85_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCQ2672644.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(18112..18618)
                     /locus_tag="OHO85_003307"
     CDS             complement(18112..18618)
                     /locus_tag="OHO85_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCQ2672645.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(18664..19164)
                     /locus_tag="OHO85_003308"
     CDS             complement(18664..19164)
                     /locus_tag="OHO85_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCQ2672646.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(19250..19429)
                     /locus_tag="OHO85_003309"
     CDS             complement(19250..19429)
                     /locus_tag="OHO85_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCQ2672647.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
BASE COUNT         5116 a         4564 c         4738 g         5129 t
ORIGIN      
        1 ccagaagggg ccgtttatgt tgccagacag cgctactgat taagcggatt ttttcgcttt
       61 tttctcagct ttagccggag cggcttcttt tttcgctgca gtttcacctt ctacataatc
      121 acgaccgtag taggtatcca gcagaatctg tttcagctcg gagatcagcg ggtaacgcgg
      181 gttagcgccg gtgcactggt catcgaatgc atcttcagac agtttatcca cgttcgccag
      241 gaagtctgct tcctgaacgc cagcttcacg gatagatttc ggaataccca gttcagcctt
      301 cagcgtttcc agccatgcca gcagtttctc gatcttagca gcagtacggt cgcccggtgc
      361 gctcagaccc aggtggtcgg caatttcagc ataacgacgg cgagcctgcg gacggtcata
      421 ctggctgaat gcagtctgct tggtcgggtt gtcgttcgca ttgtagcgaa taacgttaca
      481 aatcagcagg gcgtttgcca gaccgtgcgg aatatggaac tgggaaccca gtttgtgcgc
      541 cattgagtga catacaccca ggaaggcgtt cgcaaacgcg atacccgcga tagtcgctgc
      601 actgtgaaca cgttcacgcg ctaccggatt tttagaccct tcgtggtagg acgctggcag
      661 atattctttc agcagtttca gtgcctgcag agcctgacca tcagagaact cagatgccag
      721 tacagaaaca taagcttcca tggcgtgagt tactgcgtcc agaccaccga aagcacacag
      781 ggacttcggc atgtccataa ccaggttggc gtcgacaatc gccatatccg gggtcagcgc
      841 atagtccgcc agcgggtatt tctgaccagt agcgtcgtca gttacaaccg caaacggagt
      901 gacttcagaa cctgtaccag aagtggtggt gacagcgatc attttcgctt tcacgcccat
      961 tttcgggaac ttgtagatac gtttacggat atccataaag cgcagcgcca gctcttcgaa
     1021 gtgagtttcc ggatgttcgt acataaccca catgatcttc gcggcgtcca tcggggaacc
     1081 accacccagc gcgataatca cgtctggttt gaaggagttt gccagttctg cacctttacg
     1141 aacgatgctc agggtcgggt ccgcttctac ttcgaagaag acttcagttt caacgcctgc
     1201 tgctttcagt acggaagtga tctgatcagc ataaccattg ttgaacagga agcggtcagt
     1261 cacgatgagc gcacgtttgt ggccatcagt aatcacttca tccagcgcga ttggcaggga
     1321 gccacggcgg aagtagatag atttcggaag tttgtgccac aacatgtttt cagctcgctt
     1381 agcaacggtt ttcttgttga tcaggtgttt cggaccaacg ttttcagaga tggagttacc
     1441 accccaagaa ccacaaccca gagtcaggga aggtgcgagt ttgaagttat acaggtcacc
     1501 gataccaccc tgagacgctg gggtgttaat caggatacga gccgttttca ttttctgacc
     1561 gaagtaagaa acgcgagccg gttggttatc ctggtcagtg tacaggcaag aggtatgacc
     1621 gataccgccc atagcaacca gtttctctgc tttttctacc gcgtcttcga aatctttagc
     1681 gcggtacatt gccagagtcg gggacagttt ttcatgtgcg aacggttcgc tttcatcaac
     1741 aacggtcact tcaccgatca gaatcttggt gttttctggt acagagaagc ctgccagttc
     1801 agcaatttta taggctggct gaccaacgat agccgcgttc agcgcaccgt ttttcaggat
     1861 aacatcctga acagctttca gctctttacc ctgcaacaga tagccgccgt gggttgcaaa
     1921 acgttcacgt acagcgtcat aaacagagtc aacaacaaca acagactgtt cagaagcaca
     1981 gattacgccg ttgtcgaagg ttttggacat cagtacagat gcaactgcac gtttgatatc
     2041 agcagtttca tcgataacaa ctggagtgtt gcccgcgcct acaccgatag ctggtttacc
     2101 ggagctgtat gcggctttaa ccatgcccgg accaccagtc gcgaggatca ggttgatgtc
     2161 tgggtggtgc atcagtgcgt tagacagttc aacagaaggt tgatcgatcc agccgatcag
     2221 atctttcgga gcaccggcag cgatagcagc ctgcagaacg atatcagccg ctttgttggt
     2281 ggcatctttt gcacgcgggt gcggggagaa gataatggcg ttacgggtct tcagactgat
     2341 cagcgatttg aagatagcag ttgaagtcgg gttagtggtc ggaacgatac cgcaaataat
     2401 accgattggt tcagcgatag tgatggtacc aaaagtgtcg tcttcagaca gaacaccaca
     2461 ggttttttca tctttatagg cgttgtagat atattcagaa gcaaagtggt ttttgatcac
     2521 tttatcttcg acgataccca tgccggattc ggcaacggcc attttcgcga gtgggattcg
     2581 agcatctgca gcagccagag cggcggcgcg gaagattttg tctacttgct cttgagtgaa
     2641 actggcatat tcacgctggg ctttttttac acgctctacg agtgcgttaa gttcagcgac
     2701 attagtaaca gccataatgc tctcctgata atgttaaact tttttagtaa atcatctgct
     2761 cgaatacgag agtatagtca gtgcggtgat gatttgctta acctatgaaa atcaaaagct
     2821 tactcgcgct cacactcact gtgatttact aaaagagttt aaacattaga gttattatct
     2881 ctaatgcgtc acttccaggt ggcgtaagca agattactca cttctgggta ctgattacgt
     2941 gatccaaatc aaatttttgc aaagctgaca cctttcagca tcgcttttcg ccattatagc
     3001 taacagttaa taaattgtag tatgatttgg tggctacatt agcatgtttt gcacaactag
     3061 ataacaataa cgaatgatag caattttaag tagttaggag gtgaaaaatg ctgtcaaaag
     3121 gcgtattgtc agcgcgtctt ttcaacctta tttatggcta acattatccg gcttttgctt
     3181 cggagctaac cgtgattcag accttttttg attttcccgt ttacttcaaa tttttcatcg
     3241 ggttatttgc gctggtcaac ccggtaggga ttattcccgt ctttatcagc atgaccagtt
     3301 atcagacagc ggcagcgcga aacaaaacta accttacagc caacctgtct gtggccatta
     3361 tcttgtggat ctcgcttttt ctcggcgaca cgattctgca actttttggt atatcaattg
     3421 attcgttccg tatcgccggg ggcatcctgg tggtgacgat agcgatgtcg atgatcagcg
     3481 gcaagcttgg cgaggataaa cagaacaagc aagaaaaatc agaaaccgcg gtacgtgaaa
     3541 gcattggtgt ggtgccattg gcgttgccgt tgatggcggg gccaggggcg atcagttcta
     3601 ccatcgtctg gggtacgcgt tatcacagca ttagctatct gtttggtttc tttgtggcta
     3661 ttgcattgtt cgctttatgt tgctggggat tgttccgcat ggcaccgtgg ctggtacggg
     3721 ttttacgcca gaccggcatc aacgtgatta cgcgtattat ggggctattg ctgatggcat
     3781 tggggattga atttatcgtt actggtatta aggggatttt ccccggcctg cttaattaat
     3841 tcctttcaaa tgaaacggag ctgccatgct ccgtttactt cgtcattatt tttactttgt
     3901 tcccgcgcag ttatcaaaag caaaaggaat aggtaaaata ttcttctcaa attacagtta
     3961 gttataagga tttccttaac tgcttctcct caccatcatg ttattttcgc cacatcataa
     4021 tcctgggctt gctgaagaat aattgaaatg atattagtaa ttccactgcc tttggtagag
     4081 gaaagtgcta aataataatc aattgttaaa ttattgtgca tttcactact ggaactgtaa
     4141 tcagaaaaga tagacatgct tagccaatct ctatttgatt gaattgaaag atgtttgtta
     4201 aggcatggat gcaagctata gattctgata cggtcaataa aagagaattg cttaacaatt
     4261 ttgcaaaatg tattggcgag taagaaccgc atttggtact ttccgggcaa ccgccagacg
     4321 attctttatt ggtaatgaga ataattaaca attaaagagc gtcgcgaaag aataatgtgt
     4381 ctcgacaggg gagacacagt acgaatcgac ataaggtgat cgtctgaatc accagaataa
     4441 ataaagtcgg tgatagtaat acgtaacgat aaagtaacct gacagcagaa agtctccgag
     4501 cctgtgcagg gtcccaatcc gggattacac atgctggtta ataccagtaa ttataatgag
     4561 ggagtccaaa aaacaatgac caacatcacc aagagaagtt tagtagcagc tggcgttctg
     4621 gctgcgctaa tggcagggaa tgtcgcgctg gcagctgatg tacccgcagg cgtcacactg
     4681 gcggaaaaac aaacactggt acgtaacaat ggttcagaag ttcagtcatt agatccgcac
     4741 aaaattgaag gtgttccgga gtctaatatc agccgagacc tgtttgaagg cttactggtc
     4801 agcgatcttg acggtcatcc agcacctggc gtcgctgaat cctgggataa taaagacgcg
     4861 aaagtctgga ccttccattt gcgtaaagat gcgaaatggt ctgatggcac gccagtcaca
     4921 gcacaagact ttgtgtatag ctggcaacgt tctgttgatc cgaacactgc ttctccgtat
     4981 gccagttatc tgcaatatgg gcatatcgcc ggtattgatg aaattcttga agggaaaaaa
     5041 ccgattaccg atctcggcgt gaaagctatt gatgatcaca cattagaagt caccttaagt
     5101 gaacccgttc cgtacttcta taaattactt gttcacccat caacttcacc ggtgccaaaa
     5161 gccgctatcg agaaattcgg cgaaaaatgg acccagcctg gtaatatcgt caccaacggt
     5221 gcctatacct taaaagattg ggtcgtaaac gaacgaatcg ttcttgaacg cagcccgacc
     5281 tactggaaca acgcgaaaac cgttattaac caggtaacct atttgcctat tgcttctgaa
     5341 gttaccgatg tcaaccgcta ccgtagtggt gaaatcgaca tgacttataa caacatgccg
     5401 atcgaattgt tccagaagct gaaaaaagag atcccggacg aagttcacgt tgatccatac
     5461 ctgtgcactt actattacga gattaacaac cagaaaccgc cattcaacga tgtgcgtgtg
     5521 cgtaccgcac tgaaactagg tatggaccgc gatatcattg ttaataaagt gaaagcgcag
     5581 ggcgacatgc ccgcctatgg ttacactcca ccgtatactg atggcgcaaa attgactcag
     5641 ccggaatggt ttggctggag ccaggaaaaa cgtaacgaag aagcgaaaaa actgctggct
     5701 gaagcgggtt ataccgcaga caaaccgttg accatcaacc tgttgtataa cacctccgat
     5761 ctgcataaaa agctggcgat tgctgcctct tcattgtgga agaaaaacat tggtgtaaac
     5821 gtcaaactgg ttaaccagga gtggaaaacg ttcctcgaca cccgtcacca gggtactttt
     5881 gatgtggccc gtgcaggctg gtgtgctgac tacaacgaac caacttcctt cctgaacacc
     5941 atgctttcga acagctcgat gaataccgcg cattataaga gcccggcctt tgacagcatt
     6001 atggcggaaa cgctgaaagt gactgacgag gcgcagcgca cagctctgta cactaaagca
     6061 gaacaacagc tggataagga ttcggccatt gttcctgttt attactacgt gaatgcgcgt
     6121 ctggtgaaac cgtgggttgg tggctatacc ggcaaagatc cgctggataa tacctatacc
     6181 cggaatatgt acattgtgaa gcactaatgg caatacgtgg ggcaggagtg tcctgctcca
     6241 cggtgtctga tttttatcgc attacagaag gcacaggcca gaaggtaggg caatgttaaa
     6301 atttattcta cgtcgctgtc tggaagcgat tccgacgcta tttattctta ttactatttc
     6361 gttctttatg atgcgcctcg cgccgggaag cccttttacc ggcgaacgta ctttaccgcc
     6421 tgaagtgatg gccaatatcg aagcgaaata tcatcttaat gatccgatca tgacacagta
     6481 tttcagctac ctgaaacaac tggcgcacgg tgatttcggt ccatcgttta aatataaaga
     6541 ttattcggtc aatgacctgg tggcatccag ttttcccgtt tctgccaaac tgggagccgc
     6601 agcatttttc cttgcggtaa tactgggtgt tagtgctggc gttattgccg cattaaaaca
     6661 aaacaccaaa tgggactata ccgtgatggg gctggcaatg accggggttg ttatccccag
     6721 ttttgtggtt gcgccattat tagtcatgat atttgcgatc attttgcatt ggctgccggg
     6781 cggtggctgg aatggtgggg cgcttaaatt catgatattg ccgatggtgg cgttgtcact
     6841 cgcctatatc gccagtattg cgcgtattac ccgtggctct atgattgaag tattacactc
     6901 caattttatt cgtactgccc gggcgaaagg gttacctatg cggcggatca ttttacgcca
     6961 cgcattaaaa cctgctctgt tacccgtgct ctcctatatg ggccccgcat ttgtcggcat
     7021 tattaccggt tctatggtta tcgaaaccat ttatggtttg ccggggattg ggcaactgtt
     7081 cgttaatggc gcattgaacc gtgactattc cttagtgtta agcctgacca tcctggttgg
     7141 tgctttaacc attttgttta atgccattgt cgatgtgcta tatgcggtta tcgacccgaa
     7201 aatccgttac tgatactgga gctcgcgatg atgttaagta agaaaaacag cgagacgctg
     7261 gaaaatttca gtgaaaagct ggaggtcgaa gggcgcagct tgtggcagga cgcacgtcga
     7321 cgttttatgc ataaccgtgc ggcggttgcc agtctgatag tgctggtgct gatcgcgtta
     7381 tttgtaatcc tggcaccgat gctttcgcag tttgcctatg acgatactga ctgggcgatg
     7441 atgtccagcg ccccggatat ggagtccggt cactactttg gtactgactc atccggtcgc
     7501 gacctgcttg tgcgcgttgc gattggcggg cgtatctcac tcatggtcgg tgttgctgcg
     7561 gcactggtgg cagtggtcgt cgggacactt tatggttcgc tttccggtta tctgggcggt
     7621 aaagtggatt cggtaatgat gcgtctgctg gaaatcctca actccttccc attcatgttc
     7681 ttcgtcattt tgctggtgac ctttttcggt caaaacatcc tgctgatttt cgtggcgata
     7741 ggcatggttt cctggctgga tatggctcgt attgtgcgtg ggcaaaccct gagtctgaag
     7801 cgcaaagagt ttattgaggc ggcacaagtt ggcggtgtat cgacatcggg cattgttatt
     7861 cgccacattg tgccgaatgt actcggtgtg gtggtggtct acgcatcact tctggtgccc
     7921 agcatgatcc tctttgaatc tttccttagc ttcctggggt tgggtacgca agagccgtta
     7981 agcagctggg gtgcattgct gagtgatggc gcgaactcga tggaagtctc tccatggtta
     8041 ttgttgttcc cagcgggatt cctcgtggtg acgctgtttt gtttcaactt tatcggcgat
     8101 ggcttgcgtg atgccctcga cccgaaagat cgttaaggag tgcagccatg agcgtaattg
     8161 aaactgcaac tgtgccgctc gcacaacaac aggctgacgc actgctgaac gtgaaagatt
     8221 tgcgtgtcac ctttagtacc ccggacggcg acgtcacggc ggtcaatgat ttgaattttt
     8281 ccctacgtgc cggagaaacg ctgggtattg taggtgagtc tggttcgggt aaatcgcaaa
     8341 ctgcatttgc gttgatgggc ctgttggctg ccaacggacg tattggcgga tcggcaacct
     8401 tcaatgggcg tgaaatcctc aatttgccag agcatgaact caataaactg cgcgctgaac
     8461 aaatctcaat gatttttcag gacccaatga cttcgttgaa tccctatatg cgcgtcggtg
     8521 agcagttaat ggaagtgctg atgctgcata agaacatgag caaagctgag gcgtttgaag
     8581 agtcggtgcg gatgctcgat gcggtaaaaa tgccggaagc gcgtaaacgc atgaaaatgt
     8641 acccgcacga attttctggc ggcatgcgtc agcgagtcat gattgcgatg gcattgctat
     8701 gtcgacctaa gctactgatt gcggatgaac ccactactgc gctggacgtc accgtacagg
     8761 cgcagatcat gacgctatta aatgaactga agcgggaatt taataccgcc atcattatga
     8821 tcacccacga tcttggcgtg gtggcgggga tctgtgacaa agtgctggta atgtacgccg
     8881 ggcgcacgat ggaatatggc aatgcgcgcg atgtctttta tcaacccgtt catccttatt
     8941 ctatcggttt gctcaacgcg gtgccgcgtc ttgatgcgga aggtgaaaca atgttgacca
     9001 tcccgggtaa tccgccaaac ttgctgcgat taccgaaagg ttgcccgttc cagccacgtt
     9061 gtccacatgc gatggaaatt tgtagtagcg caccgccgct ggaagagttt acgcttggcc
     9121 gtctgcgtgc ttgctttaaa ccggtggagg aactgttatg aatgctgtaa ctgaaggaag
     9181 aaaagtcctc cttgaaatcg ccgatcttaa agtgcacttt gaaatcaaag atggcaaaca
     9241 gtggttctgg caaccgccga aaacgctcaa agccgtcgat ggtgtcactc ttcgcctgta
     9301 tgaaggggaa acattaggtg tggtagggga atcgggatgc ggtaagtcca cctttgctcg
     9361 cgccatcatc ggtttggtca aggcgaccga cggtcatgtt gcctggttag gtaaagagtt
     9421 gctgggcatg aagcccgatg aatggcgtgc cgttcgcagt gatattcaga tgattttcca
     9481 ggatccgttg gcatcgctaa acccgcgtat gaccatcggc gagatcatcg ctgaaccact
     9541 gcgtacttat catccgaaaa tgtcacgcca ggaagttcgc gagcgcgtga aggcgatgat
     9601 gctgaaagtc gggttattgc ctaacctgat taaccgctat ccgcatgagt tctccggtgg
     9661 gcagtgccag cgtatcggga ttgctcgtgc tcttattctt gaaccgaagc tgattatctg
     9721 cgatgagccg gtttcggcgc tggacgtgtc aattcaggcg caggtggtca acctgctcca
     9781 gcagctgcaa cgtgagatgg gattgtcatt aatttttatc gctcatgacc tggccgtggt
     9841 aaaacacatt tccgatcgtg tgttggtgat gtatctcggc catgcggtag aactggggac
     9901 ctatgatgag gtctaccaca atccactaca tccttacacc agggcattga tgtcggcagt
     9961 ccccatacct gatccggatc tggagaagaa caaaaccatc cagttactgg aaggggaatt
    10021 accgtcgccg atcaacccgc cttccggttg tgttttccgt acccgttgcc cgattgccgg
    10081 tccggagtgc gccaaaacac gtcctgttct ggaggggagt ttcagacacg ccgtttcttg
    10141 cctgaaagtc gatccgcttt aaacacaata agggctgaca gttgtcagcc ctttttcacg
    10201 ctaaaagcga ttatttattc ccgccagatg atatggcaaa gtttgtgatc tttttcgcgg
    10261 cataacagaa tgcgggcgaa aacatcgttg atttcaccat cttcactgtc cgccagacca
    10321 atcaccactt cggcaaaaaa gtctgggttc aaatcgaaat caacatgttc ctgccagtct
    10381 tccgccggat cgaataactc cgcgccgccg cgctcttcaa actgaagatt gaacagcaga
    10441 acatcagctg gatcgagatt gtcggcagcc agttcgagaa aaatatcgta ggcctgctca
    10501 agcgtttcat cttcagtcag gcgattgttt agatccatat ccatagtcac tacctgttta
    10561 acctctgttg gcgacgtttt acagcaacgg actgaagaag taaaacagtc gctcggcgac
    10621 acgttgccat aatggacgtt ttagccataa acgggcatcg agcagacgtg aacgcgaaat
    10681 ataatcgtcc tgaacggcgg caaggtctgc accaaaacct ttatcgtcga ttgccagggt
    10741 aatctcgaaa ttcagccaca gactacgcat atcaaggtta acggtgccaa ccagacttag
    10801 ttcgccatcg accagcacgc tcttggtatg cagtaacccg ccttcaaact gataaatttt
    10861 aaccccagca gcaagaagtt ccgtaaagaa tgcgcgactg gcccagccga ccagcatcga
    10921 gtcatttttt cgcggaagga taatactgac atccaccccg cgctgcgccg ccgtgcaaat
    10981 cgcatgaagt aaatcatcgc ttggcacaaa gtagggcgtg gtcatgatca aatattcacg
    11041 cgccgaataa gccgcagtca ataatgcctg gtgaatgaga tcttccggaa agccggggcc
    11101 agaagcaatt gtgtgaatgg tgtgaccgct ggcctgttca aacggcataa tattgacatc
    11161 tggtggtggc ggcagaatac gttttccggt ttcaatctcc cagtcgcagg aataaataat
    11221 ccccatcgcg gtggcgatgg ggccttccat acgcgccatc agatcaatcc attgccctac
    11281 gcccgcatct tgtttgaagt agcgaggatc gaccatattc atgctgccgg tgtacgcgat
    11341 gtaattatcg atcatgatca tcttgcgatg ttggcgcagg tccatacggc gtaaaaacac
    11401 acgcatcaga ttgaccttta aggcttcgac cacttcaata ccggcattac gcattagctc
    11461 gggccacggg ctgcggaaaa aagccacact cccggcggag tcgagcatca atcggcaatg
    11521 aataccacgc cgtgcagccg ccattaatga ttcagccacc tggtccgcca tgccgccggg
    11581 ctgccagata taaaacacca tctcaatatt atggcgcgcg agctggatgt cgcggattaa
    11641 cgcctgcatc acatcatctg actcggtcat cagttgtagc tgattccctt tgaccccagc
    11701 gatcccctga cgacgctcgc aaagcttgaa taatggcgca gcgacactgc tattttcttc
    11761 ggcgaagata tgcttacagg ctttaaggtc gttaagccat tttgcggtgg aaggccacat
    11821 cgctctggcg cgctcagcgc ggcgtttgcc taaatggagc tcgccaacgg caagataggc
    11881 aataattccg actaacggca gaatgtaaat aatcaacagc caggccatcg cggagggaac
    11941 tgcgcgtcgt ttcattagaa tgcgtaaagt tacgcctgca atgagcaacc agtatcccag
    12001 aatggccaac caactcacca acgtataaac ggttgtcata gattagaaat ccttttgaaa
    12061 gcgcattgtt atgagtttac gcatatggat tcatctggca aataaaaacg cgggaaaagc
    12121 actggtcagc gtcgggggtg ggggtataat gaccattctg ttattgcata gagtagttaa
    12181 catgaagcgg agtagaacgg aagtggggcg ctggcgcatg cagcgtcagg ctagccgacg
    12241 taaatcgcgt tggcttgagg ggcaatcgcg ccgaaatatg cgtatccaca gcatcaggaa
    12301 gtgcattcta aacaaacagc gtaactcgtt attgtttgcg atctacaata tctaaatgta
    12361 ctgggcacca ttgcggtgcc caataagttt actcttccgt ctttaaactc tatttttgcg
    12421 ccgattcttt actgtcagct ttcgtttctt gttcatcgtt atcgctgaaa ataccatgac
    12481 ctgagttcaa caacattgaa acgagcatat cattattaat ctcttcacca tttaaaactc
    12541 gcgccagaat ttcgctgcca aacagttgcg gtgagagaat gatatccgga tgtaccatct
    12601 taatcttatt caggtttttg ctatcactga cggcgagaac tgttttgaca tcactgctca
    12661 tatcttttgc cgagagtaca acaaacgcgt tatctgcatc gttatcactc agcgccagaa
    12721 tggcccggca acgatcgatt cccgctttct ttaatactga actgtcatta ctgtcaccgg
    12781 ggataacatc agcgttatcg cctaagcgtt gctcaagttg cttgatatca tcttcaggca
    12841 agttgctgat aaccgttacg ttttgtccgc gttgattcag ttgcaggatc gtattgatgg
    12901 cgagaatcga atgtccgcaa acaataaaat gatctttacg atgcattgta tgattgtttc
    12961 cttttacaag tttgttgaat cccccgcgga taagcggacc aaaaattgag gtcatagatg
    13021 tggcaaaaac ggtaatgccg gaaataatga ccgaaatagt gaacaatcgt gctgattcag
    13081 aaacagggac aatatcgcca tagccgacgg ttgacatggt ttctatcgaa aaatagaacg
    13141 cggtcatcaa actttctatt cgcggattaa aaccttcgct taaataaagc gcaccgtagg
    13201 ttgagtaaaa cagtaatgtc gtgaaactaa taaatgcaaa aattgtcccg gctgcggcgc
    13261 tactgtggga gaagtctttg cgcagtatca gcaaaaacac cagcgtaaaa atgcaaaatc
    13321 caatactaaa tttcagccag ggataaaaat gcagggtgta aattagcgct atcaacaaca
    13381 aaatgatact gattgcccat gcgagctttg cccggaacag taagccaatg gagttcagga
    13441 caagaaagac gccgagcata aatagtgggg cgtttgccag caaggagaga tccaactctg
    13501 aaaatgcttt gatatggaaa atatcaagca aattgaccga cataccatag attgttttaa
    13561 aaataagcaa tccatttaaa aagacggcca gcgcgagaat gtcgtgccga agggtcaccc
    13621 ataaatttgt tgctgtttgt ttgaatgtag cccagtgact cactgatacc tctgtaacca
    13681 caattttgtt taattatctt agcgtgcaca ttttctgaac atacatgcag cgcgtgaatg
    13741 tgttaaacaa tagtagatga ctttatccgc agtaatcaaa ggatttgagc gagcaactgt
    13801 acctcaggaa aggtcatttt tcctgaatat gctcacatca tataaagaaa tacagataaa
    13861 gttattatct gcttgtggta gtgaatgcac tgaccggcta taaggaaagg ccaaacaaga
    13921 acacggttgc aaaaaccgtg cccttaaata ttgaatctct attcagaaca ctttcttaaa
    13981 tggtttcact gaaacgtgtt catagactcc tgccgctacg tacgggtcag catcggccca
    14041 ggcctgagct gcttccagcg attcaaattc agcaataacg gttgagccag taaatcccgc
    14101 agcccctgga tcgttactgt ctaccgctgg cattggacca gctgtcaaca aacgaccttc
    14161 atcatgcagt aactgtaaac gtgctaaatg tgccggacga acggaaaggc gtttttcgag
    14221 gctatcagct ttgtcttgag cgtaaataac atacaacacg ggcacaactc cttgttcggg
    14281 aaagttgtaa agtacgttat ttgaaagggc gaagatctgc aacggaaaga tgatgtcttt
    14341 gttaagacca tgcataaatt aaggtcaatt acgccaaaaa tgacattttc actgatcctg
    14401 atcgtcttgc cttattgaat atgattgcta tttgcattta aaatcgagac ctggtttttc
    14461 tactgaaatg attatgactt caatgaccct tgatttacct cgccgcttcc cctggccgac
    14521 gttactttcg gtctgcattc atggtgctgt tgtggcgggt ctgctctata cctcggtaca
    14581 tcaggttatt gaactacctg cgcctgcgca gccaatttct gtcacgatgg ttgcgcctgc
    14641 tgatctcgaa ccgccacaag ccgttcagcc gccaccggag ccggtggtag agccagaacc
    14701 ggaacctgag ccgatccccg aaccaccaaa agaagcaccg gcggtcattg aaaagccgaa
    14761 gccgaaacct aagccaaaac cgaagccggt gaaaaaggta caggagcagc caaaacgcga
    14821 tgtcaaaccc gtagagtcgc gtccggcatc accgtttgaa aatacggcac ccgcacgcct
    14881 gacatcaagt acggcaacgg ctgcaaccag caagccggtt accagtgtgg cttcaggacc
    14941 acgcgcatta agccgtaatc agccgcagta tccggcacga gcacaggcat tgcgcattga
    15001 agggcaggtt aaagttaaat ttgacgtcac gccggatggt cgcgtggata acgtacaaat
    15061 cctctcagcc aagcctgcga acatgtttga gcgtgaggtg aaaaatgcga tgcgcagatg
    15121 gcgttatgag ccgggtaagc caggcagtgg gattgtggtg aatatcctgt ttaaaattaa
    15181 cggcaccacc gaaattcagt aagcagaaag tcaaaagcct ccgaccggag gcttttgact
    15241 attactcaac aggtaaggcg cgaggttttc cttcaggatc aaccgcgaca tacttaaata
    15301 atgcttctgt cgctttatag cgttgcccga ttggttcaga cgctactttt ttcacccaca
    15361 cttcaatatt aatgctgacc gatgtcgtcc ctttctggac acagcgtgca tagcagcaca
    15421 ccacatcgcc gaccgcaacc ggccgtaaga aagtcattcc ttcaacccgc acagtcacta
    15481 cgcgaccgtg ggcaatttct ttcgccagaa tagcgccgcc aatatccatt tgtgacatta
    15541 accaaccacc aaagatgtca ccattggcat tggtatcggc gggcatggct aaagtacgta
    15601 aaacaagatc gccctgaggg acgttatgtg ttgtagacat ggtaaaaccg acttaaaagg
    15661 aaaatcacag gcgcgatgct actatgattt tcctgaggag aacagaggag atacagcgcc
    15721 agccccgaag gactggcagt ctggttagga tttatcttcc tgcggcatgt ggcggtagat
    15781 atagataccg cttaacaatg tgaagattaa ggtaagggcg gtcaggccaa agactttaaa
    15841 gttgacccaa atattttgcg gcagccagaa tgcgatgtag atgtttgcca gaccgcaaag
    15901 gataaagaaa acagcccaag ccagatttag cttcgaccat accggttgcg gcagcgtgag
    15961 ttctttaccc agcatccgct gaattagcgg ctttttcatc acccattggc tgactaacag
    16021 ggctcccgca aacagagcat aaatgactgt aaccttccat ttaataaact catcattgtg
    16081 gaagaacagc gtcaagccac cgaagacgac caccagaaca aaagtgatca gggccatctt
    16141 ctcaacctta cgaaagcgaa cccagctata gataagcaca atcgccgtgg cgacgatcag
    16201 cgccgcagta gccgcataga tgtcataaat cttgtaaaac gcgaaaaaga caaccagcgg
    16261 taaaaaatca agaaactgct tcattttacg attccgtaat caaatgctta aggttattgg
    16321 cgaatcaaca tatacaggcg gaacagatag atgagcaata tggcagaaat caggttgctc
    16381 aaggtgttcg ccagtacggc accaatttcc ggggttaatg cggcaaaaga agaggcaaaa
    16441 agcagcaaca gtgtttttgc cagcaaccag ctcagtactg cgggtgccac cagacgcata
    16501 ttcgcccaag tcagccgcat actgctacgc atcgaggcaa aaatgcccat tttgtcctgt
    16561 accagcatca ccggtgccag ggccagtaaa atggccatga taattcccgg aacgaccacc
    16621 agcatgatgc caatctgtac taaaagggta gtcagaaaaa tcagaataaa taacttgggc
    16681 aatatcggcg cactggcacc aatagctcgc agcgcactga ctctctgacc cgcagacacc
    16741 agctggataa ttaatattac gcctccggcg agaatggcgt taccgattaa tcctgaaaaa
    16801 gtggacgccg ctgaagcctg cagcaaaatt tgttgctgtt ccggtgacat attctgaacc
    16861 aggtcgaaca acccactact gccgctaacg ggcacgccgt cattgagctg cgcaagctgt
    16921 gcatcactgg gtgagaaaac atgccctaac accactgtga taaacgcaca tagcaacgat
    16981 accaacagaa tggtcataaa ttgattacgg aagaaatttc cggtgtcacg gtatacggac
    17041 tgcgccgtga tagacatgca ctctccttga gttttatgca ggtgttaatt agcgggcaat
    17101 tgtaccctgg ttaagcctcg ggtggcagca tcaggtcttg tatggaaaag catatctttg
    17161 taaagcggag gtaattgctg gccttcaggc gtgggcaaac cgcggcaagt ggcgatcgag
    17221 ttaatatttg cgtagagaaa atatttaaaa attgatttaa atcacattaa ccaggattct
    17281 caatgcaact tctaaattaa tccagatcaa taaatggtga attatcatat gtaatgtgat
    17341 ctatgtagga tcatttgtta ctccaatgta ggtatattcg tcacgttttt ataaccataa
    17401 cgacggagcg gatatgaaaa agttaacagt ggcggctttg gcagtaacaa ctcttctttc
    17461 tggcagtgcc tttgcgcatg aagcaggcga attttttatg cgtgcaggtt ctgcaaccgt
    17521 acgtccaaca gaaggtgctg gtggtacgtt aggaagtctg ggtggattca gcgtgaccaa
    17581 taacactcaa ctgggcttga cgtttactta tatggcgacc gacaacattg gtgtggaatt
    17641 actggcagcg acgccgttcc gccataaaat cggcacccgg gcgaccggcg atattgcaac
    17701 cgttcaccat ctgccgccaa cactgatggc gcagtggtat tttggtgatg ccagcagcaa
    17761 attccgtcct tacgttgggg ccggtattaa ctacaccacc ttctttgata atggatttaa
    17821 cgatcatggc aaagaggcgg ggctttccga tctcagtctg aaagattcct ggggagctgc
    17881 cgggcaggtg ggggttgatt atctgattaa ccgtgactgg ttggttaaca tgtcagtgtg
    17941 gtacatggat atcgatacca ccgccaatta taagctaggc ggtgcacagc aacacgatag
    18001 cgtacgcctc gatccgtggg tgtttatgtt ctcagcagga tatcgttttt aattccacaa
    18061 aaaaacgacc ccgtaatata cggggtcaat aaggacatgg cataaagtgt attatttctt
    18121 cgcttctacg ccgtcagttt cagagcgaat taaaaatttc tcagttgttt gcggaatatg
    18181 ctgaatcagc cagtcggcca tttgcttttc ctcatttaaa atcgcttcga tggttggaat
    18241 tgaagctgta tcaccggcat tttttgctgc tgctaatagt gaggtgtaac aggcgatttc
    18301 aaattgctcg aagacatatc cgctaataga gcctttgact atttcatcag aagggaatat
    18361 accaccgatt gactgcccaa gcgcagccat tttactcatg gaatctttaa tgactgaacg
    18421 tgaaatgtca ttacgatcaa gaatagtttc cagttgaaca atctggtttt tggtttcact
    18481 aagatgttgt tcaatacgag cgcgtagttc aggataatta tctatacggc tggccatgga
    18541 ttcaagcata gattcggctt gcttttccat tgcgtgggcg tcacgtaacc agtcatgata
    18601 atgttcaata cgattcatga aaatgtcctc tttataaaat gactaaaagt taaagttcat
    18661 atttcaggct ttattttcgg ctttcttatt tacgttatta agggccagat cagtcagttt
    18721 gatgtcggtg gccttttctt cttccagggt ctctttcaga agcttcgctg ctttacgata
    18781 acctaattgt tcagccagcg tcgctaatgt cccgtaactg gcaatctcat aatgctcgac
    18841 tttctgtgct gcggcaatca gtgcggcatc acgcacttcg tttttctcgg tactttcgat
    18901 gacctcatta gcttcttcaa taagaccttc cattgccaca catttcatgc gcttaatttt
    18961 cagattcgat tctgattcca caacttgatc aatacgttca atctgtccat gtgtttcctc
    19021 gaggtgcgca tgaaaagcct gacttaattt ttcgtttgat gttgctcttg cgagttttgc
    19081 cagtgcccgg gttaattgtt tttctgcgct gtaggtatct gaaagcaggt gaataaatac
    19141 atcttcaatg gtcttcatat tcatattttt ctccagtgaa atcactgcga gccatacggc
    19201 ccgcagatgc aattatcttt atcagctgat attaataatt aacggtgaat caggatttgc
    19261 ctgatttatt accaccgctt tgttgaccgc cttttttacc cgcttcagat gcgcgttgcg
    19321 gatcattttt aaaattaccg ccgctatgct gaccgccttt acggcctgcg tcggatgcct
    19381 tctcacggtc ttcggcgaaa tttcctgaac caccacgatg ttcggccatg ttatttctcc
    19441 cgttgcgttg cattgtttca ttaatatgag tgttgtgtgt cgacactcat taaaattagt
    19501 cgctaatgag aattagtcaa attaagcgca acgagaagat agaggga
//
LOCUS       DAJOWB010000087        19058 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJOWB010000087 DAJOWB010000000
VERSION     DAJOWB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.087"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..134)
                     /gene="tuf"
                     /locus_tag="OHO85_003310"
     CDS             complement(<1..134)
                     /gene="tuf"
                     /locus_tag="OHO85_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCQ2672648.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     R"
     gene            complement(205..2319)
                     /gene="fusA"
                     /locus_tag="OHO85_003311"
     CDS             complement(205..2319)
                     /gene="fusA"
                     /locus_tag="OHO85_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCQ2672649.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="OHO85_003312"
     CDS             complement(2416..2886)
                     /gene="rpsG"
                     /locus_tag="OHO85_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCQ2672650.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="OHO85_003313"
     CDS             complement(2983..3357)
                     /gene="rpsL"
                     /locus_tag="OHO85_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCQ2672651.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="OHO85_003314"
     CDS             complement(3483..3770)
                     /gene="tusB"
                     /locus_tag="OHO85_003314"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCQ2672652.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3778..4137)
                     /gene="tusC"
                     /locus_tag="OHO85_003315"
     CDS             complement(3778..4137)
                     /gene="tusC"
                     /locus_tag="OHO85_003315"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCQ2672653.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4137..4523)
                     /gene="tusD"
                     /locus_tag="OHO85_003316"
     CDS             complement(4137..4523)
                     /gene="tusD"
                     /locus_tag="OHO85_003316"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCQ2672654.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4523..5245)
                     /locus_tag="OHO85_003317"
     CDS             complement(4523..5245)
                     /locus_tag="OHO85_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCQ2672655.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5412..6224)
                     /gene="fkpA"
                     /locus_tag="OHO85_003318"
     CDS             complement(5412..6224)
                     /gene="fkpA"
                     /locus_tag="OHO85_003318"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCQ2672656.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6445..6663
                     /locus_tag="OHO85_003319"
     CDS             6445..6663
                     /locus_tag="OHO85_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCQ2672657.1"
                     /translation="MQDLSLETRLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6712..7302)
                     /gene="slyD"
                     /locus_tag="OHO85_003320"
     CDS             complement(6712..7302)
                     /gene="slyD"
                     /locus_tag="OHO85_003320"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCQ2672658.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7397..7597)
                     /locus_tag="OHO85_003321"
     CDS             complement(7397..7597)
                     /locus_tag="OHO85_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCQ2672659.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7607..9412)
                     /gene="kefB"
                     /locus_tag="OHO85_003322"
     CDS             complement(7607..9412)
                     /gene="kefB"
                     /locus_tag="OHO85_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCQ2672660.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9412..9963)
                     /gene="kefG"
                     /locus_tag="OHO85_003323"
     CDS             complement(9412..9963)
                     /gene="kefG"
                     /locus_tag="OHO85_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCQ2672661.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10094..12007
                     /locus_tag="OHO85_003324"
     CDS             10094..12007
                     /locus_tag="OHO85_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2672662.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQADEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12007..13029
                     /locus_tag="OHO85_003325"
     CDS             12007..13029
                     /locus_tag="OHO85_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCQ2672663.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13023..13241
                     /locus_tag="OHO85_003326"
     CDS             13023..13241
                     /locus_tag="OHO85_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCQ2672664.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13295..14164
                     /locus_tag="OHO85_003327"
     CDS             13295..14164
                     /locus_tag="OHO85_003327"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCQ2672665.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14219..14623)
                     /locus_tag="OHO85_003328"
     CDS             complement(14219..14623)
                     /locus_tag="OHO85_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCQ2672666.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14925..15557
                     /gene="crp"
                     /locus_tag="OHO85_003329"
     CDS             14925..15557
                     /gene="crp"
                     /locus_tag="OHO85_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCQ2672667.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15608..17698
                     /locus_tag="OHO85_003330"
     CDS             15608..17698
                     /locus_tag="OHO85_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCQ2672668.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFAACSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17765..18985)
                     /gene="argD/dapC"
                     /locus_tag="OHO85_003331"
     CDS             complement(17765..18985)
                     /gene="argD/dapC"
                     /locus_tag="OHO85_003331"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCQ2672669.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTSAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
BASE COUNT         4685 a         5190 c         4730 g         4453 t
ORIGIN      
        1 cgagcagcac cgccgtaggt tttagccagt acggtggtga ttgcagcggt cagagtagtt
       61 ttaccgtggt caacgtggcc gatagtacca acgttaacgt gcggttttgt acgttcaaat
      121 ttttctttag acacggctat attccttact atagtgctct ccccttcagg agagagcacg
      181 ggactttggt attaaccctt aggcttattt accacgggct tcaattacgg cctgagcaac
      241 gttactcggc gcttcatcat acttcaggaa ttccatagtg tatgatgcac gacctttggt
      301 cagagaacgc agctgagttg cgtatccgaa catttcagac agcggtactt cagcgtggat
      361 cttaacgcca gtaacttcag attcctgacc tttgagcata ccacgacgac ggctcaagtc
      421 accgataacg tcaccggtgt tctcttccgg agtttctact tcaaccttca tgatcggctc
      481 aagcagaact ggtttcgctt tcttaaagcc ttctttaaag gcgatagaag cagccagttt
      541 aaacgccagt tcagaggagt caacgtcatg gtaagaaccg aagtgcagac gaatacccat
      601 gtctactacc gggtagcctg ccagcggacc tgctttcagc tgttcctgga tacctttatc
      661 aacggccggg atgtattcgc cagggattac accaccttta atgtcgttga tgaactcgta
      721 gcctttcggg tttgaacccg gctccagcgg gtacatgtcg ataacaacat gaccatactg
      781 accacgacca ccagactgtt tcgcgtgttt accttcaaca tcggtaactt tctggcggat
      841 agtttcacgg taagcaacct gcggtttacc tacgttcgct tcaacgttga attcacgctt
      901 catacggtca acgatgatgt cgaggtgcag ttcgcccata cccgcgatga tggtctggtt
      961 agattcttcg tcagtccata cacggaaaga cgggtcttct ttagccagac ggcccagagc
     1021 cagacccatt ttttcctggt cagctttggt tttcggttca actgcgatgg agattaccgg
     1081 ctcagggaat tccatacgtt ccagaatgat cggcgcatcc gggtcacaca gggtgtcacc
     1141 agtggttacg tctttcagac cgatagcagc agcgatgtcg cccgcgcgaa cttctttgat
     1201 ctcttcacgt ttgttagcgt gcatctgaac gatacgaccg aaacgctcac gtgcagcttt
     1261 cacggagttc agtacggtat caccagagtt aaccacaccg gagtaaacac ggaagaaggt
     1321 caggttacca acaaacgggt cggtagcgat tttgaacgcc agtgcagaga acggctcgtc
     1381 atcacttgcg tgacgttcag ccggagtgtc tttaccgtcg tccaggatac cgttgatcgc
     1441 aggtacgtca accggggatg gcaggtaatc aattaccgca tccagcatcg cctgaacacc
     1501 tttgttcttg aacgcagaac cacaggttac caggatgatt tcgttgttca gaacgcgctg
     1561 acgcagagca cctttgattt ctgcttcagt cagttcttca ccacccaggt atttttccat
     1621 cagctcttca gaagcttcag ctgcggattc gatcaggttc tggtgccatt cgttagccag
     1681 ttcaaccatg tctgccggga tatcttcgta ttcgaaggtt acgccctggt cagcgtcgtt
     1741 ccagttgata gctttcattt tcaccaggtc aacaacaccg gtgaaatgtt cttcagcacc
     1801 aatcgccagc tgcagcggaa ccgggttcgc gcccagacgg gttttgatct ggttaacaac
     1861 tttcaggaag ttcgcaccca tgcggtccat tttgttaacg aacgcaatgc gcggaacttt
     1921 atatttgttt gcctgacgcc atacggtttc agactgcggc tgaacaccac caactgcgca
     1981 gtaaaccatt accgcaccat cgagaacacg catggaacgt tctacttcga ttgtgaagtc
     2041 aacgtgcccc ggggtgtcga tgatgttgat gcgatgcggc tcatactgct tagccatacc
     2101 agaccagaat gcagtagtcg cagcggaagt gatggtaata ccacgttcct gctcctgctc
     2161 catccagtcc atggttgcag cgccgtcatg aacttcaccg attttatggt ttacaccggt
     2221 gtagaacaga atacgttcgg tagtagtggt tttaccggcg tcgatgtgcg cactgatacc
     2281 gatgttacgg tagcgtgcga tgggtgttgt acgagccatt tgtttcctcg tttatctttt
     2341 aggcgttcaa tttaagtagc ccaaagcggg ctgcttactg gaagcgcccg cctggtgact
     2401 aaaactccga agggattacc aacggtagtg tgcgaacgcc ttgttggctt cggccatacg
     2461 gtgaacgtct tcacgtttct taactgcagt acctttgttt tctgcagcat cagaaagttc
     2521 gttcgccagg cgcagagcca tggatttatc accgcgttta cgagcagctt caacgatcca
     2581 acgcattgcc agagcattac gacgaaccgg acggacttca actggtacct gataagtaga
     2641 accaccaacg cggcgagact taacttctac agtcgggcgc acgttttcga gagctacttc
     2701 gaatgcttcc agttcagatt taccagagcg ctgagccagg gtctccagcg cgctgtatac
     2761 gatagattca gcagtagatt ttttaccatc taccatcagg atatttacaa atttagccag
     2821 cagttctgat ccgaacttcg gatccggcag aattttacgc tgaccaatga cgcgacgacg
     2881 tggcatggaa atactccgtt gttaattcag gattgtccaa aactctacga gtttagtttg
     2941 acatttaagt taaaacgttt ggccttactt aacggagaac cattaagcct taggacgctt
     3001 cacgccatac ttggaacgag cctgcttacg gtctttaacg ccggagcagt caagcgcacc
     3061 acgtacggtg tggtaacgaa cacccgggag gtctttaaca cgaccgccac ggatcaggat
     3121 cacggagtgc tcctgcaggt tgtgaccttc accaccgatg taggaagtca cttcgaaacc
     3181 gttagtcaga cgaacacggc atactttacg cagcgcggag ttcggttttt taggagtggt
     3241 agtatataca cgagtacata cgccacgttt ttgcgggcat gcttccagcg caggcacgtt
     3301 gcttttcgca actttgcgag cacgtggttt gcgtaccagc tggttaactg ttgccattaa
     3361 atagctcctg gttttagctt ttgcttcgta aacacgtaat aaaacgtcct cacacaatat
     3421 gaggacgccg aattttaggg cgatgccgaa aaggtgtcaa gaaatataca acgatcccgc
     3481 catcaccagg ccatctggct ggagtgctta accgtaagtc tgacgaaatc agtatagtca
     3541 atgggaatga tgtcgttcga aatttgacca gtcaaaccgc gggcaataag gtcttcgttc
     3601 agggcataga ccttaatggg ggcattacgc agactttcaa ggtagcggtt accgtcaact
     3661 gcggcagtta cgccatcttg caataatagc agttcgtctc cttcactgag cagacgcagc
     3721 agcgcagcaa aatccgtcag ccagggtgaa cgatgtaacg tgtgcagcat aaacgcctca
     3781 aaacctcaaa ataacatcgt agttggcgag ttcgcggcgt aaggcatctg cttcgagcgg
     3841 cgtggcttcg acaacaaagg gtgtctgcgg atctaacccg cgttcgcgca gtgaagccgc
     3901 acaaacccag cactgttcaa tgtcgtacag acccaacaat ttaaaagtgg caatgtaatc
     3961 acgcgccagc actgcatcgg gcttttgtcc tgacagcagc tgaaaaacgc catcagcaat
     4021 aaagaagacg gccagatcgt cagttaatgc ggaagttgcc agtaaagcat ctaaaccttc
     4081 ccgaccagca gctgtaccat gaggtgcagt agaaaaaaca aacgcaattc gtttcatcag
     4141 aactgtacca ccctgtcaca ggtcagcgag gcttccgcca gcgcgccaag tccgcttaag
     4201 gtaaatcccg gctgaaggtt tgacgaagcc agccccagcc ttcctgcttc agtttcatcg
     4261 acgataccgc ggcgtaacgc tgccgctacg cagatattca gcgccacacc atgttgcgca
     4321 ttcaactgtt gccagccgcg cacaaggtca aattcgtcac ttgccggtga ggtcaattgg
     4381 ttagcgttat agaccccttc ccgatagaaa aagacgctgc ttaactcatg gccttctact
     4441 atcagcgcct gcgcaaactg aaaagcacta ctcgcctgtt gcgtaccgta tgctggcccg
     4501 gtcaccacga tggcaaaacg cattacttat cttgcccctg gaaatcaccg cttttgaact
     4561 ggcggatgta gagatagaca gtgtgtttgg agatgttcag gcggtcagca acctggttga
     4621 tcgcgtcttt aatatcgaag atccctttct cgtagagatt cagcacgatc tgacgatttt
     4681 tggcgttatt agaaacattg cgatcggcat ttacttcttc gatggtgaac tccagcgttt
     4741 gggtaaccag atcttcaaca gaagaggcaa agttgacgct tgaaccgaca tccggggttt
     4801 ctggcggcac aaaagtgctc ataatctgcg agaagggaac atcaagattc atattgatgc
     4861 acagcagacc aattacacgc tgttcgcggt tacgaatcgc gatagtcagg gacttcatta
     4921 atacgccgct tttggcgcga gtaaagtagc atttagaaac gctgctatcc gctcccgtca
     4981 tatcgtgcag catacgtagc gccaggtcag taattggcga accaatcttc cggcctgtat
     5041 gttcaccgtt agcaatgcga atggctgaac attttagatc ctgcaaagag tgcaaaacga
     5101 tttcacagtg ggagccaata agcatcgcta acccgtccac caccgcttcg taggatttca
     5161 gaatatcaaa atcggtctgg tcgaaaggac gttgatccag taaatccaac tcactggttt
     5221 cgttggttaa aagcgacctg gacatgaaaa aaaacactcc ttttcaggag cctgtcgtta
     5281 acttttcagg gcaggctcat taatgatgcg ggtaactaaa ttaatacagc ggaggttccg
     5341 ctttccagca ctaattatat ccggcctgta ataaaaaaac cgccgcctgg tcaggcggcg
     5401 gttcttaatg cttatttttt agcagaatct gcggctttcg catcagcttc cggctttgca
     5461 tcagccttcg gcgctggttt cacgtccaac agctctacgt caaacaccag ggtagaattc
     5521 ggtgggatcc ccggaacacc cgctttgccg taagccagtt ctggtggaat aaccagtttg
     5581 atcttaccgc ctttcttgat gttcttcaga ccttctgtcc aacccgggat aacaccgtcc
     5641 agacggaaag agagcggttc accacgagtg taagagttgt cgaactcttt accgtcgatc
     5701 agagtacctt tgtagttcac gacaacagta tcgctgtctt tcggtgcttc gcctttaccg
     5761 gcttctacta cctgataaac cagaccagtt gaagaggttt tcacaccttt ctctttggca
     5821 aatttctcgc ggtactcttt accttttgct tcgttatcag ccgcgtcttt ttccatcttc
     5881 gcctgagcag aagacttcac gcgagcttcg aatgcttgca gagtctgttc gatctcttgg
     5941 tcggagagtt tgctcttatc agcaaatgca tcctgaacac cagcgatcag ctgatcttta
     6001 tccagtttga tgcccagttt ttcttgttct tttagagagt tttccatgta acgacccagc
     6061 gaggcaccca gtgcataagc tgatttctga tcgtcatttt tgaacgctgc tttgctgtca
     6121 gcagttgtag caggttttgc agcttcagca gcaaaagtga ttggtgcatg cagggcaacg
     6181 gccattgtgg tcgccagcag cgttacttta aacagtgatt tcatccatat ctccaggatc
     6241 ggggcatctc accccagggt taactattat cagaagggta ctataaagcg ttgtcgaaca
     6301 aatctacata cagacacgcc ctattatcat ctattttcag actctttttg tttaaattag
     6361 tttcgatgac cgcgaaatga gtgctgtctc gggcagcaaa gttaagtaga atccgcggcg
     6421 accattcgac aaaagaggtg aatcatgcag gatttatcat tggaaacacg cctggcagag
     6481 ctggagagcc gactggcttt tcaggaaatc accattgaag aactgaacgt cacggtgacc
     6541 gctcatgaaa tggagatggc gaaactgcgc gatcatctgc gtctgctgac cgagaagtta
     6601 aaagccagcc agccgtcgaa catcgcgtcg caggctgaag aaacgccacc gccacattat
     6661 tgaggcgtaa aaaaagcggg gattccccgc ttttttgtca ctttttcggt attagtggca
     6721 accgcaaccg ccgttgcctt taccgccaca gcagccttcg ccaccgtgtt catgaccgtg
     6781 atcgtgacca tgaccgccgc agcaaccgtc gtggtcgtga tcgtggtggt gatcgtgcgc
     6841 gccgtgaacg tgaccatgag ccagttcttc ttcagtcgct tcgcgaatcg ccacaacttc
     6901 aacgttgaat ttcaggttct gaccggccag catgtggtta ccatcaacca cgacgtgatc
     6961 gtcttcaacc gcagtgattt caaccggtac cggaccctgg tcggtttcag ccaggaaacg
     7021 catacctacc tgcagttcat caacgcccat aaatacgtct ttaggaacac gttgcaccag
     7081 gttttcgtcg tactgaccgt aagcgtcgtt cgcgccaaca gcgacatcaa atttgtcgcc
     7141 aacttcatga ccttccagcg ccgtttccag gccagagatc agggaaccgt gaccatgcag
     7201 gtagtccagc ggcgcactca ccggagactc atcaaccaac acaccgtctt ctgtacgtac
     7261 ctgataggcc aggctgacca ccaggtcttt tgctactttc atgatatctc ctgagcatgg
     7321 gaagaatagt ggcgcagatt gtagcggaat tctgcagccg tgtactcact agcttaaaaa
     7381 aactcggcgc atatcgctag tccggatgaa aaatcccgat cacttgctca tctttgcgaa
     7441 cgtgatcgcg ggcttctttg tctgcttctc gcatctgatg tccgcactta acacattcaa
     7501 caatatcaat attattttcg cgccacatcg ccattgaatc ctgcgcctga caggccgggc
     7561 attttgcgcc cgcaataaaa cgttttcgga ttgccatctt taccctctac tcaaattcat
     7621 cccagccgtc cagctggcgt cgttcttgtt gcatttcacg ctggaaaatc tcttccagtt
     7681 cgcgtcgggc ttccctggcg cgagaaattt gtacggtatc ggcatgcatt gggatgagct
     7741 ctcgcagcat ccgcatatcc aggcggcgaa aatgcagttg cgctcgctgt gcctgatgcg
     7801 gatgcatgcc aagcgtgacc agcgtcttgc gccccagctc taacgcactg gagaatgttt
     7861 cacgggaaaa ctgcgtcacc cctgcctgta ataactcatg cgcttccaca cgtccgcgcg
     7921 ctcgcgcaag aatatgcaaa tgcggaaagt gctgctggca tatttccacc agcttcatgg
     7981 tgtcttccgg ctcgttacag gtaatgacga tagactcagc ggcctctgca cccgcagaac
     8041 gtaaaagatc gacctgcgtg gcgtcgccgt aataaacttt gtagccgtat ttacgcatca
     8101 ggttaacggc gctgatatcc cgctccagca cggtaatgcg cattttattt gccatcagca
     8161 aacgaccaat cacctgacca aaacgcccga agcccacgac aatgacctgg ggtttatcat
     8221 cgttgaccca cggtttttcg tcttcttctt ccggtccgtt aaactggcgg gatagccatt
     8281 tatccaccag cttcatcagc aacggcgtgg tcatcatgga aagcgtcacc gtcaccagca
     8341 acaacgccat ctggtcgccc tggaataagc gttgtgaaga agcggtagaa aagaggacaa
     8401 aggcaaactc accaccctga ctcaacacgc cagcaaactg catccgctct gagctacgca
     8461 cgccatacaa tcgcgccagc agatacagca cgagaatttt caccgccacc agcacaacca
     8521 cgcttatcac tacccacaac agatgggtat aaagcacccc gaggttgagt gacatgccga
     8581 cagagataaa gaacaaaccg agcagcaagc ctttgaaggg atcgatagcc gtttccagtt
     8641 catggcgata ttcactttcc gccagcagca cgcccgcaat aaatgtaccg agcgccatcg
     8701 acagccccag cgcatccata aacaatgcgg aacccaacac cagcagcagc gtcgcggcgg
     8761 tgaacacttc ccgcacgcca gaagctgcaa taaagcggaa taccggacgc agtaaatagc
     8821 gcccaccaat cagcatgccg acaaacgcca gcaccttcat gccgatcttc atccagtcga
     8881 aatgttcgtc tgccgacccc gccaacaacg gcactaacgc cagtgctggg attactgcca
     8941 gatcctgaaa cagcagaacc gaaaacccga gctggccgga ttcgctgcga ttcatccctt
     9001 tctcacgcat caattgcaat gccattgcag ttgaagacat cgcaaggcca atgccaccga
     9061 ccaccgccgc ctgccaggcg aaatccgtca gcatcaataa tcccgccagc aacgccgcgc
     9121 ttaacagcac ctgtgccgcg cctacgccaa aaatcgaacg ccgcagttgc caaagtttgg
     9181 agggattcaa ctcaaggccg atgataaaca tcaggaatac cacgccgagt tccgaaaagt
     9241 ggaggatctc atcgacgtcg ctaataaacc ccagccccca cgggccaatt gcaatccctg
     9301 ccagcaaata tcccaacaca gcgccaatac ccagccgcga tgccagcggc accgcagcca
     9361 ccgccgcgaa gagaaacagc actcctgcga gtaaaaaatc ggaaccttcc atcagcggcc
     9421 tcctggagac agcggatttg ccagccagtc accgtaggct ctggcgtggc tcgccagctc
     9481 ctgtgcgctt tgccgtctcg cccagtaaat aatgatggga cttaaccaat gcatccggca
     9541 catgcccgcc gccagttcaa aggggcgcag cacatcgctc atcgggtagc gattcagcgc
     9601 gtcataacgg taagcacttt ctggctcgcc ggtggtaatc acgctacgcc agtactttcc
     9661 cgccagttgg tttcctcccg gcccgctggc aaaaccacga cttaataccc ggtccagcca
     9721 ctctttcagt agcgccgggc agctataggt ataaagagga tgctgaaaga caatcacctc
     9781 gtgctcgcgc agtaatgcct gctcacgggg gatatcaata aaaaaatcgg gatagtgcgc
     9841 gtaaaggtcg tgcacggtaa cattgctgag ctgcgtggcc ggtttaagca gtacccggtt
     9901 tgccaccgag tcctgagatt ccggatgggc atacagcagc aaaactttcg ctggctgaga
     9961 catcatcccc ctcccggtat ggtttttgtg tatagtcgct gttttgggct accattgcgc
    10021 ccggtgcggc agctcgccca tacattacat tatcataatg ataagttaac atagtctgaa
    10081 catacggcac cttatgattg ttttctcctc gttacaaatt cgtcgcggcg tgcgcgtcct
    10141 gctggataat gccaccgcca ccatcaaccc agggcagaaa gtcggcctgg tgggtaaaaa
    10201 cggctgtggt aaatctaccc tgctggcatt gctgaaaaat gaaatcagcg ccgacggcgg
    10261 cagctacacc tttccgggaa gctggcaact ggcgtgggtg aatcaggaaa cgccggcgtt
    10321 accgcaagcg gcgctggaat atgtcattga cggcgaccgt gaatatcgtc aactagaagc
    10381 gcagctacac gacgccaacg aacgtaacga cgggcacgcc attgcgacca ttcatggcaa
    10441 gctggatgct attgacgcat ggagtattcg ctcccgtgct gccagcctgc tgcacggcct
    10501 cggtttcagc aatgaacaac tggagcgccc ggtaagtgat ttttccgggg gctggcgtat
    10561 gcgtcttaac cttgcccagg cgctgatttg ccgttcagac ttgctgctgc tcgacgaacc
    10621 gactaaccac ctcgatctcg atgccgttat ctggctggaa aaatggttga agagctatca
    10681 gggcacgctg atcctgatct ctcacgaccg cgacttcctc gatccgatcg ttgataaaat
    10741 tattcatatc gaacaacaaa gcatgttcga gtacaccggt aactacagtt cgtttgaagt
    10801 acagcgcgcc acccgtctgg cgcagcaaca agcgatgtat gaaagccagc aggaacgcgt
    10861 agcgcatctg caaagttata tcgaccgttt ccgtgccaaa gccaccaaag cgaagcaggc
    10921 ccagagccgc attaagatgc tcgagcgtat ggagctgatt gcccccgcgc acgtcgacaa
    10981 cccgttccgc tttagcttcc gcgcgccgga aagcctgcca aatccgttac tgaagatgga
    11041 aaaagtcagc gcgggctatg gcgatcgcat tattctcgac tcgattaaac tgaacctggt
    11101 gcccggctcg cgcattggtc tgttaggccg caacggcgcg ggtaaatcga cattaatcaa
    11161 actgttagcc ggtgaacttg cgccagtcag cggtgaaatt ggtctggcga aagggatcaa
    11221 gctcggctac ttcgcccagc atcaacttga atacctgcgc gccgacgaat cacctattca
    11281 acatctggca cgtttagcgc cgcaggagct ggaacaaaaa ctgcgtgact acctcggcgg
    11341 ctttggtttc cagggcgata aagtaaccga agaaacgcgc cgcttctcag gtggggaaaa
    11401 agcccgcctg gtgctggcat taattgtctg gcagcgtccg aatctgctgc tgctcgacga
    11461 accgactaac caccttgacc tcgacatgcg tcaggcactc accgaagcat taatcgagtt
    11521 cgaaggcgcg ctggttgtcg tttcgcacga ccgtcatttg ctgcgttcca ccactgacga
    11581 tctctacctg gttcacgatc gtaaagtcga accgttcgac ggcgatctgg aagattatca
    11641 acagtggttg agcgacgtac aaaagcagga aaaccaggcc gacgaagcgc caaaagagaa
    11701 cgcgaacagc gcccaggcac gtaaagatca gaagcgtcgg gaagcggagt tgcgtgcgca
    11761 aacccagcca ctgcgtaaag agattgcccg tctggaaaaa gagatggaga agctgaacgc
    11821 gcaactggcg caggcagaag agaaactcgg cgacagcgaa ctgtatgacc agagccgtaa
    11881 agcggagttg accgcctgcc tgcaacagca agccagcgcc aaatccggcc tggaagagtg
    11941 cgaaatggca tggctggaag cccaggagca gcttgagcag atgttgctgg aaggccaaag
    12001 caactgatgg cgcagataac gacgaccgat gccaatgaat tcagcagcag tgctgaattc
    12061 acccctatgc gcggctttag caattgtcat ctgcaaacca tgctgccgcg tctgtttcgt
    12121 cgcaaggtga aattcacccc gtactggcag cggctggagt tgcccgacgg cgattttgtc
    12181 gatctcgcgt ggagtgaagc ccctgcacag gctcggcata aaccgcgttt agtggtattt
    12241 cacgggctgg aaggcagtct caatagccct tacgcccacg gtctggttga ggcggcgcaa
    12301 aagcgcggct ggctgggcgt ggtgatgcat tttcgcggat gcagcggtga accaaaccgt
    12361 atgcaccgca tttaccattc gggcgaaacc gaagacgcca gttggttttt acgctggctg
    12421 caacgcgaat ttggtcatgc gccaacggct gccgtcggct attcgctcgg cggtaatatg
    12481 ctggcctgtt tgctggcaaa ggaaggcaat gatctcccgg ttgatgcggc ggtgattgtc
    12541 tctgcgccgt ttatgctgga agcctgtagc tatcatatgg aaaagggctt ttcccgcgtt
    12601 tatcagcgtt acttgctgaa cctgttaaaa gccaatgccg cgcgcaagct ggcagcctac
    12661 cccggaacgc tgccgattaa tctcgcgcag ttaaaatcgg tacgtcgcat ccgtgaattt
    12721 gacgatctaa tcaccgccag aattcacggc tacgccgacg ctatcgacta ttatcgtcag
    12781 tgtagcgcca tgccgatgct gaaccggatc gccaaaccga cgctgattat tcacgccaaa
    12841 gacgatccgt ttatggatca tcaggtgatc ccgaaaccgg aaagtctccc ctcgcaggtg
    12901 gagtatcaac tgactgaaca tggcggtcat gttggcttta ttggcggtac attacttcat
    12961 ccgcaaatgt ggctggagtc acgcattcct gactggttaa caacgtatct ggaggcgaaa
    13021 tcatgttgat tccgtggcaa gacctctccc ccgaaacgct ggaaaattta attgaaagct
    13081 ttgtgttacg tgaaggcacc gattatggtg aacatgagcg tacacttgaa cagaaagtcg
    13141 ccgacgtcaa acgccagcta cagtgcggag aagcggtgct ggtatggtcg gagctgcacg
    13201 aaacggtcaa tattatgccg cgcagtcagt ttcgcgaata acctgcaacc gtcggcggac
    13261 gagataataa tgatagctaa gtcatggagt tactatgtct gccaaacatc cggtcattgc
    13321 ggtaacagga tccagcggcg cggggaccac caccaccagc ctcgcgtttc gtaaaatatt
    13381 cgcgcagtta aatctgcatg cagctgaggt ggaaggcgac agttttcacc gttacacccg
    13441 cccggaaatg gacatggcga tccgcaaagc gcgcgacgcc gggcggcata tcagctactt
    13501 cggccccgaa gcgaacgact tcggcctgct ggaacaaacc ttcattgaat acggtcagag
    13561 cggcaaaggg aaatctcgca aatatctgca tacctacgac gaagccgtac cctggaatca
    13621 ggtaccggga acattcaccc cctggcaacc tttaccagaa cccactgatg tactgtttta
    13681 tgaaggttta cacggcggcg tagtcacgcc acagcataac gttgcgcagc atgtggactt
    13741 actggtcggc gtagtgccta tcgttaacct tgagtggatt caaaaactga tccgcgacac
    13801 cagcgagcgc gggcactcac gagaagcagt gatggactcg gtagtgcgtt caatggaaga
    13861 ctatatcaac tacatcacac cgcagttttc ccgcacccat cttaacttcc agcgcgttcc
    13921 caccgtcgac acttcaaacc cgttcgcggc aaaaggtatc ccgtcgctgg atgaaagctt
    13981 tgtggtgatc cattttcgta atctggaagg gatcgatttc ccctggctgc tggcgatgtt
    14041 gcaaggctca ttcatttccc acatcaatac gttagtggtg cccggcggca aaatgggtct
    14101 ggcaatggaa ttaattatgc tgccgctggt gcaacgattg atggaaggaa agaaaatcga
    14161 gtaactctgc tattacgccg gataaaatac tatccggctt cacaacggga tagttaaatc
    14221 acgcggcaac cacttcatac gagtgagtaa tattcaccgc tttttccagc atcaacgcca
    14281 ctgaacaata tttctcagca gagagatcaa ccgcacgcgc aaccgctgcg tctttcaggt
    14341 cgcgaccggt gacgataaaa tgcagattaa tatgcgtaaa caggcgtggt gcctcttcgc
    14401 ggcgttcaga ggtcaatttt acttcacaat cgaccacatc ctgacgccct ttttgcagga
    14461 tcgaaaccac atcgatggca ctgcaaccac ccgccgccat caacaccatt tccatcggac
    14521 ttggtgcttt atcgcctgag ttgccgtcca ttaaaatctg atgaccagag gcggattcgc
    14581 ccagaaaagt taacccttcg acccacttca ctcgcgcttg catttttgct actccactgc
    14641 gtcaattttc ctgacagagt acgcgtacta accaaatcgc gcaacggaag gcgacctggg
    14701 tcatgctgaa gcgagacacc aggagacaca aagcgaaagc tatgctaaaa cagtcaggat
    14761 gctacagtaa tacattgatg tactgcatgt atgcaaagga cgtcacatta ccgtgcagta
    14821 cagttgatag ccccttccca ggtagcggga agcatatttc ggcaatccag agacagcggc
    14881 gttatctggc tctggagaaa gcttataaca gaggataacc gcgcatggtg cttggcaaac
    14941 cgcaaacaga cccgactctc gaatggttct tgtctcattg ccacattcat aagtacccat
    15001 ccaagagcac gcttattcac cagggtgaaa aagcggaaac gctgtactac atcgttaaag
    15061 gctctgtggc agtgctgatc aaagacgaag agggtaaaga aatgatcctc tcctatctga
    15121 atcagggtga ttttattggc gaactgggcc tgtttgaaga gggccaggaa cgtagcgcat
    15181 gggtacgtgc gaaaaccgcc tgtgaagtgg ctgaaatttc gtacaaaaaa tttcgccaat
    15241 tgattcaggt aaacccggac attctgatgc gtctgtctgc acagatggcg cgtcgtctgc
    15301 aagtcacttc agagaaagtg ggcaacctgg cgttcctcga cgtgacgggc cgcattgcac
    15361 agactctgct gaacctggca aaacaaccag atgctatgac tcacccggac ggtatgcaaa
    15421 tcaaaattac ccgtcaggaa atcggtcaga ttgtcggctg ttctcgtgaa accgtgggac
    15481 gcattctgaa gatgctggaa gatcagaacc tgatctccgc acacggtaaa accatcgtcg
    15541 tttacggcac tcgttaatcc cgtcggagtg gcgcgttact gagtagcgcg ccattttgtt
    15601 tcccccgatg tggcgcagac tgatttatca ccccgatatt aactatgcac tgcggcaaac
    15661 gctggtgctc tgtttgcccg tggccgttgg gttaatgctt ggcgaattac gattcggtct
    15721 gctcttctcc ctcgttcctg cctgttgcaa tattgcgggc cttgatacgc ctcataaacg
    15781 ttttttcaaa cgcttaatca ttggtgcgtc gctgtttgca gcctgtagct tgctgacaca
    15841 gctgctactg gcaaaagatg ttcccctgcc ctttttgctg accggattaa cgctggtact
    15901 tggcgtcact gctgagttgg ggccattgca cgcaaaattg cttcccgcat cgctgctggc
    15961 agccattttt accctcagtc tggcgggata catgccggtc tgggaaccgt tgctcatcta
    16021 tgcgttgggc actctctggt acggattgtt taactggttt tggttctgga tctggcgcga
    16081 acaaccgctg cgcgagtcac taagtctgct gtaccgtgaa ctagcagatt attgtgaagc
    16141 caaatacagc ctgcttaccc agcacaccga ccctgaaaaa gcgctgccgc cgctgctggt
    16201 gcgccagcaa aaagcggtcg atctaattac ccagtgctat cagcaaatgc atatgctttc
    16261 cgcgcaaaat aatactgact acaagcggat gctgcgtatt ttccaggagg cgctggattt
    16321 acaggaacat atttcggtca gtttgcatca gccggaagag gtgcaaaagc tggtcgagcg
    16381 tagccatgcg gaagaagtta tccgctggaa tgcgcaaacc gtcgccgctc gcctgcgcgt
    16441 gctggctgat gacattcttt accatcgcct gccaacgcgt tttacgatgg aaaagcaaat
    16501 tggcgcactg gaaaaaatcg cccgccagca tccggataat ccggttgggc aattctgcta
    16561 ctggcatttc agccgcatcg cccgcgtgct gcgcacccaa aaaccgctct atgcccgtga
    16621 cttactggcc gataaacagc ggcgaatgcc gttacttccg gcgctgaaaa gttatctgtc
    16681 actaaagtct ccggcgctac gcaatgccgg acgactcagt gtgatgttaa gcgttgccag
    16741 cctgatgggc accgcgctgc atctgccgaa gtcgtactgg atcctgatga cggtattgct
    16801 ggtgacacaa aatggctatg gcgcaacccg tctgaggatt gtgaatcgct ccgtgggaac
    16861 cgtggtcggg ttaatcattg cgggcgtggc gctgcacttt aaaattcccg aaggttacac
    16921 cctgacgttg atgctgatta ccaccctcgc cagctacctg atattgcgca aaaactacgg
    16981 ctgggcgacg gtcggtttta ctattaccgc agtgtatacc ctgcaactat tgtggttgaa
    17041 cggcgagcaa tacatccttc cgcgtcttat cgataccatt attggttgtt taattgcttt
    17101 cggcggtact gtctggctgt ggccgcagtg gcagagcggg ttattgcgta aaaacgccca
    17161 tgacgcttta gaagcctatc aggaagcgat tcgcttgatt cttagcgagg atccgcaacc
    17221 tacgccactg gcctggcagc gaatgcgggt aaatcaggca cataacactc tgtataactc
    17281 attgaatcag gcgatgcagg aaccggcgtt taacagccat tatctggcag atatgaaact
    17341 gtgggtaacg cacagccagt ttattgttga gcatattaat gccatgacca cgctggcgcg
    17401 ggaacaccgg gcattgccac ctgaactggc acaagagtat ttacagtctt gtgaaatcgc
    17461 cattcagcgt tgtcagcagc gactggagta tgacgaaccg ggtagttctg gcgatgccaa
    17521 tatcatggat gcgccggaga tgcagccgca cgaaggcgcg gcaggtacgc tggagcagca
    17581 tttacagcgg gttattggtc atctgaacac catgcacacc atttcgtcga tggcatggcg
    17641 tcagcgaccg catcacggga tttggctgag tcgcaagttg cgggattcga aggcgtaatg
    17701 caggctaaac catcggataa ggcattcacg ccgcatccga catcttttgc ctgatgcttc
    17761 gtatttacgc cccaaccacc ttcgccaccg cgtgggcgaa acgttgcatc ccttcatcga
    17821 tatccgcatc ttccaccacc agcgacggtg caaaacgcat cacatccggt ccggcattca
    17881 gcaccattac gccagcctct gcgcccgcat acaggaaatc acgcgcccga cctttgtact
    17941 gtggtttcag ctctgcgcca atcaacagcc ccataccgcg aatatcgcta aacacatcgt
    18001 actgctgatc gatcttctgc agatggtcaa caaaacgctg gcgtttcgcc tgaatgcctt
    18061 ccagcacttc aggggtattg atgatatcaa acgccgcccc cgctactgca caggccagag
    18121 gattaccgcc gtaggtggaa ccgtgagaac caggatgaaa cgcagaagca atttccgccg
    18181 tggtcagcat ggcgctaatc gggaagccgc cgcctaacgc tttcgcagag gtcagaatat
    18241 ccggcgtaac gccgtagtgc atgtaagcaa acaaatcgcc ggtccgcccc atcccgcact
    18301 gcacttcatc aaacaccaat aatgcctgat gttgatcgca cagctcgcgc aagccctgca
    18361 aaaactctgg cgtcgctgcc gtcacaccgc cctcgccctg gatcggctca accaccaccg
    18421 cactggtgtg atcatccatc accgctttca ctgcgtggag atcgttaaaa ggaacgtgga
    18481 tgatgtctgc cggttttggc ccaaagccgt cggaatattt tggctgcccg cccaccgaga
    18541 cggtaaacag cgagcgacca tgaaaagcgt tatggaaggc aataattttg gttttgaacg
    18601 gactatgacg cacacaggcg taatggcgtg ccagtttaaa ggcggtttcg ttagcttccg
    18661 tgccggagtt cataaacacc acgcgttcgg caaacgtcgc ctcaatcagt ttgcgcccaa
    18721 gacgcagcgc cggttcattg gtgaaaacgt tactgatatg ccacagagtt tcgccctggg
    18781 tttttaacgc gttcaccagc gcaggatggc aatggcccaa cgccgtaact gcaatgccac
    18841 ccgcgaaatc gacatactcc ttgccttgct gatcccagat tcggctgccc tgacctttta
    18901 ccggaatgaa ctctgccgga gcataaatcg gcaggatcac ttcatcgaaa gtcgcgcgtg
    18961 taattgctgt ttgttcaatt gccatctcat gatcaccctg ttacgcataa ataaatgtga
    19021 aattataatc acaaaatatg cataaaaaat cattgaat
//
LOCUS       DAJOWB010000088        18888 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJOWB010000088 DAJOWB010000000
VERSION     DAJOWB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.088"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            182..388
                     /locus_tag="OHO85_003332"
     CDS             182..388
                     /locus_tag="OHO85_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672670.1"
                     /translation="MHKDQYTDAPSQEQVRVKTMLNSTISMGYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNTKDYSLVVD"
     gene            941..1435
                     /locus_tag="OHO85_003333"
     CDS             941..1435
                     /locus_tag="OHO85_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HCQ2672671.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            1435..3537
                     /locus_tag="OHO85_003334"
     CDS             1435..3537
                     /locus_tag="OHO85_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCQ2672672.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRILPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSILRAKLISRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPEWASRETWLECVPDSTEPSPTPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            3534..3746
                     /locus_tag="OHO85_003335"
     CDS             3534..3746
                     /locus_tag="OHO85_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672673.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            3674..5254
                     /locus_tag="OHO85_003336"
     CDS             3674..5254
                     /locus_tag="OHO85_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011703754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCQ2672674.1"
                     /translation="MGAPPCGSDYTTTGASRVPAGEVLMAILDDVIGVFSPGWKAARL
                     RSRAVIQAYEAVKTTRTHKARRENRTADQLSQYGAVSLREQARYLDNNHDLVIGVFDK
                     LEERVVGKNGIIVEPHPVLRNGAIARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLM
                     LRTWLRDGEVFAQMVSGRINSLTPSAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDD
                     WGRPEKYLVYKSRPVSGRQMETKEVDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSAL
                     KEYEDSELTAARIAAALGMYIRKGDGQSYEPDGNGSKENERELTIQPGIIYDDLKPGE
                     EIGMVKSDRPNPNLETFRNGQLRAVAAGSRLSFSSTARNYNGTYSAQRQELVESTDGY
                     LILQDWFIGAVTRPMYRAWLKQAVASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKE
                     AEAWKIQIRGGAATESDWVRAGGRNPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGS
                     SAATKRQEPQHTDDQSEE"
     gene            5199..7226
                     /locus_tag="OHO85_003337"
     CDS             5199..7226
                     /locus_tag="OHO85_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCQ2672675.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENSFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAQGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDEPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSGYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            7313..7636
                     /locus_tag="OHO85_003338"
     CDS             7313..7636
                     /locus_tag="OHO85_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HCQ2672676.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGMTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            7629..7904
                     /locus_tag="OHO85_003339"
     CDS             7629..7904
                     /locus_tag="OHO85_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HCQ2672677.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            7916..8494
                     /locus_tag="OHO85_003340"
     CDS             7916..8494
                     /locus_tag="OHO85_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2672678.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKVPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            8491..8892
                     /locus_tag="OHO85_003341"
     CDS             8491..8892
                     /locus_tag="OHO85_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2672679.1"
                     /translation="MNRHTQIRQAVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDIPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8904..9647
                     /locus_tag="OHO85_003342"
     CDS             8904..9647
                     /locus_tag="OHO85_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2672680.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVS
                     VTGLTVAPASASVKVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTSDGDFVAVAAVAVSAAG"
     gene            9708..10094
                     /locus_tag="OHO85_003343"
     CDS             9708..10094
                     /locus_tag="OHO85_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCQ2672681.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGVIENAGDLEHEVLTPEKS"
     gene            10103..10432
                     /locus_tag="OHO85_003344"
     CDS             10103..10432
                     /locus_tag="OHO85_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCQ2672682.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQLGH"
     gene            10404..13469
                     /locus_tag="OHO85_003345"
     CDS             10404..13469
                     /locus_tag="OHO85_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCQ2672683.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASVHAQTVEKSAQAHERMAREVEQTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAASVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGDQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINSMQYADQAVRDAAIQRENERYEKALASGKKKTREPRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAGTAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFAPQ
                     YNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            13469..13798
                     /locus_tag="OHO85_003346"
     CDS             13469..13798
                     /locus_tag="OHO85_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2672684.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSIY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            13808..14506
                     /locus_tag="OHO85_003347"
     CDS             13808..14506
                     /locus_tag="OHO85_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCQ2672685.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPVQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14512..15255
                     /locus_tag="OHO85_003348"
     CDS             14512..15255
                     /locus_tag="OHO85_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2672686.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLSAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNMEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLVAVSTFV"
     gene            15153..15800
                     /locus_tag="OHO85_003349"
     CDS             15153..15800
                     /locus_tag="OHO85_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCQ2672687.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     ATQLPVFRQKLNEGWYQVRIAGRDAGENELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15861..>18888
                     /locus_tag="OHO85_003350"
     CDS             15861..>18888
                     /locus_tag="OHO85_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCQ2672688.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     AITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQGYPNTALV
                     GVKVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAANAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLKAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTS"
BASE COUNT         4505 a         4452 c         5783 g         4148 t
ORIGIN      
        1 aaatctcgat tgtcgatggt attggttgtg tgacaatacc tagtctttcc ggtatgcctg
       61 aagagaatac aaacgacaga ttatgtaagg gaaacgcgct gaaactttca ctctttcatg
      121 ctgagtgatt tgacgttata tttttagccg caagtgaaga gcaaacgaat ggagcgacaa
      181 aatgcataaa gatcaataca ctgatgcccc ctcccaggag caggttcgtg taaaaactat
      241 gcttaatagc accatttcga tgggttaccc ggatgttgta attgcatgta tagaacatca
      301 agtatctctg gaagcattca gggcaattga ggccgcgtta gtaaagcacg ataagaatac
      361 gaaggattat tccctggtgg ttgactgatc accataactg ctaaccattc tgaccatttc
      421 acaagtgaca gagtcaatat cgtattctgt cactgtaagg ctaatacagt actgcaattc
      481 aactacagca atgcctcgta tttaggtgaa tttacaatat cgtccagttc ggatgccggc
      541 tgcatttctg aagataaggc atttcggttc gcgtattttc ccctcatgct cgtcagtcct
      601 gcgcgtagta agaaacagga cactcacact aatttgtgtg ggcatgctgt gatgtccttc
      661 tgaattatcc ccatgccatt atgtaacgct ctgtttcaga tgctcgtcac ggttgtcagg
      721 ctgtcgggtc ctcccagtgg gggtccctgc cacggggcgg gagcgtcgcg gaaaaaggct
      781 agtttttgaa attttattcg tcatcaccac tactgtaatt gattgatatt acagtggttt
      841 tatttttatg gtgtcgattc tgattgtttt ttgttcatca ctaacaccgt ttgcctaaag
      901 ttgttcgcaa gatgcatgtt taaaacattc tggagcgggt atggatcgag agttaaaaaa
      961 tctgacgctg aatatcagtc aactggcggc attgtcaggt gtacatcgcc agactgctgc
     1021 ggcaaggctg caaaatctac ccgttgcagg ggggcatgaa agcaacctca agctttatcg
     1081 ggtggttgat attgtgtcgg catttctggc attaccaccg ccggttgcag aaggcgaaat
     1141 ggacgcgcat gagcgcaaag cctggtatca gtctgaacgt gagcgtctta agttcgaaca
     1201 ggaaacggca caactcattc cggccagtga tgtcagacgg gagtttgcca tctgggcaaa
     1261 agcggtcgtg caggtgctgg agacattacc ggatattctt gaacgtgact gcggtctgca
     1321 gcctgccgct gtgagccgtg ttcagtccat tattgatgat ctgcgcgatc agatagccct
     1381 gcgggtgact gaagcaggtg cggatgatga ggaggaatta cagcaggagg agtaatgctg
     1441 aatcaggaaa ccgcaaaggc agcacgaacc gattcaggtt atatccttcg cgcaccgaga
     1501 cgaatgcggg ttgctgatgc cgttgctcag tatatgcggg tgcctatggg ggcagggaac
     1561 tcagtcccgt gggatccgct ggtggcaccg tatgttattg agcctatgaa ctgcctggcc
     1621 tcgcgtgaat acgacgcagt gatatttgtt ggcccggcac gaaccggcaa gactatcggc
     1681 ctgattgacg gctgggtgat ttacaacgtg atttgcgatc ctgctgatat gctgatcatt
     1741 cagatgacgg aggaaaaagc ccgcgaacac tccaaaaaac gactcgccag aacgtttcgc
     1801 gtcagcccgg aagtggtcag tcgcctgagt ccgaacaaaa atgacaacaa cgtttatgac
     1861 agaacattcc ttgctggcaa ctacctgaaa atcggctggc cgtcagtcaa tatcatgtct
     1921 tcatcagatt ataaatgcgt ggcgctgacg gattatgacc gttttccgga agatattgat
     1981 ggtgaggggg atgccttctc tcttgcctca aaacgtacca ccacatttat gtccagtggt
     2041 atgacgctgg tggagagttc ccccggcagg gatgtgaagg atgtgaaatg gcgacggact
     2101 tcaccgcatg aggctccacc aaccacgggg atcctgtcgc tctataaccg tggcgatcgc
     2161 cgtcgctggt actggccctg tccacactgt ggtgagtatt ttcagccctg cggcgatgtg
     2221 gttgctggtt tccgtgatat tgccgatcct gtgctggcaa gtgaggcggc ttatattcag
     2281 tgtccttcct gttcaggacg gattttgcct gaacaaaaac gcgagctgaa cggacgtggg
     2341 gtctggttac gggatggtga atccatcaat gcagatggca gtcgttatgg tgatccccgg
     2401 cgttcacgta ttgcgtcatt ctggatggag ggtccggcag ctgcttatca gacactctcg
     2461 caactcgttt acaaactgct tactgcagaa caggaatacg agacaaccgg aagtgaagag
     2521 acactcaaga cggttatcaa taccgactgg ggattacctt atcttccccg cgccagcatg
     2581 gagcaacgaa aaagtgaatt gcttgagcag cgggcagagc cagttccttc ccgcagtgtg
     2641 ccggatggcg ttaatttcct tgtggcgaca gtggatgtgc aggcgggacg tcatcgccgt
     2701 tttgtggttc aggtaacggg ctatggcagc cgtggcgaac gctggattat tgatcgttac
     2761 aacatcacgc agtcattgcg cggtgacagc gacggggaga gccagcgaat tgatccggcc
     2821 agctatccgg aagactggga tgtcctgctg acggatgttt ttcataaaag ctggccgctg
     2881 gcctccgatc cttctcaaca aatgcgactg atggcaatgg cggtggactc cggcggtgaa
     2941 gacggggtca ctgataatgc ctataaattc tggcgtcgtt gccgtcgtga tggccttggt
     3001 aaacgtattt acctgtttaa gggtgacagc atcctgcgcg caaaactgat cagccgtaca
     3061 ttccctgata acaccggacg aacgggccgc cgggcgcagg ccgcaggtga tgtgccgctc
     3121 tggcttcttc agacggatgc cctgaaagac cgggtgaata acgcgttatg gcgtgactcg
     3181 ccaggtcccg gctatgtgca tttccctgac tggctgggga gctggtttta cgacgaactg
     3241 acgtatgaag agcggagcag tgacgggaaa tggagtaagc cgggtcgcgg tgccaacgaa
     3301 gcttttgacc tgatggtgta tgccgaggct ctggtcattc tgcatggata cgaaaagatc
     3361 cgctggccgg atgcaccgga gtgggcgagc cgggaaacct ggctggagtg tgtcccggac
     3421 agtaccgaac cgtcacccac accggaaccg gtatccacgc ctgttaaaaa acaaaaacgg
     3481 aagaaaacag taactgacga tgttaacccc tggctgactt ccggaggatg gttatgaacc
     3541 agaatgatat cgaagccatg attcagcgtt atacggaagc tgaaatggcg gtgctggacg
     3601 gaaaatccgt cacctttaat ggtcagcaga tgaccatgga aaacttatct gagatccggc
     3661 agggacggca ggagtgggag cgccgccttg cggctctgat tacacgacga cgggggcatc
     3721 ccgggtaccg gctggcgagg ttctgatggc aattcttgat gatgtgattg gcgttttttc
     3781 accaggatgg aaagcggcaa ggctgcgttc ccgtgcggtg atccaggctt atgaggccgt
     3841 aaaaacgaca cggacacaca aagcccggcg ggaaaaccga actgccgacc agttaagcca
     3901 gtacggggcc gtgtcgttac gtgagcaggc ccgttacctt gataacaacc acgatctggt
     3961 tattggtgta tttgacaagc tggaagaacg ggtggtgggg aaaaacggga ttattgtcga
     4021 gccacatccg gtattacgca atggggccat tgcccgtgat ctggcagcgg agatacgcac
     4081 ccgatggagt gaatggtctg tcagtccgga agtcaccggg cagtttaccc gtccgatgct
     4141 ggaacgtctg atgctgcgta cctggctgcg cgatggtgag gtgtttgccc agatggtttc
     4201 cgggcgcata aacagcctga cgccttctgc cggtgttcat ttctggctgg aggcgctcga
     4261 gccggacttt attcccatga ccagtgatga gagcaacagg ctgaatcagg gcgtgtttgt
     4321 tgatgactgg gggcgtcccg aaaaatatct ggtgtataaa agccgtcccg tatccgggcg
     4381 gcagatggaa accaaagaag tggatgcaga gcgaatgctg catcttaaat ttgttcgccg
     4441 tctgcaccag atgcgcggga cgtctttgtt gtccggtgtg ctgatccgcc tcagtgccct
     4501 gaaagagtat gaagattctg agctgactgc agcaaggatc gccgctgctc tggggatgta
     4561 catccggaaa ggcgacgggc agagctatga accggatggt aatggcagca aggagaatga
     4621 acgcgagctt accattcagc caggcattat ttacgacgat ctgaaacccg gcgaagaaat
     4681 cggaatggtg aagtcggatc gtcccaatcc taaccttgaa acttttcgta atggtcagtt
     4741 gcgtgccgtg gcggcgggca gtcgtctgag tttttccagt acggcacgca actataacgg
     4801 cacttacagc gcccagcgtc aggagctggt tgaatccact gatggctacc tgatcctgca
     4861 ggactggttt attggtgccg tcacccgccc gatgtatcgt gcctggctga aacaggctgt
     4921 ggcatccggt gttatcaggc taccccgcga tcttgatcgt tcttcactgt ataccgcggt
     4981 gtattccgga ccggtgatgc cgtggattga ccctgttaag gaggctgagg cctggaaaat
     5041 tcagattcgt ggtggagcgg cgacagaatc agactgggta cgtgcaggtg gtcgtaatcc
     5101 ggatgatgtc aaacgtcggc gcaaggccga aattgatgaa aaccgcaagc tggatctggt
     5161 atttgatacc gatccggcca gtgataaagg aggcagcagt gccgcaacga aacgacagga
     5221 gccgcagcac accgacgacc agtccgaaga ataattcctg gttcaggatg caggcaggtc
     5281 accagagtga cgcggatatt tatatttatg acgagattgg tttctggggt gttacagcga
     5341 agcagtttat cagtgatctg aatgcactgg gcgatatcac ccacattaat ctccatatta
     5401 attcaccggg tggcgatgtc tttgaaggca tcgccatttt taatgcactg aaaacacatg
     5461 gtgcgtccat taccgtttat gtcgacggtg tggcggcgtc aatggcgtcg gtcattgcga
     5521 tggtgggaaa cccggtcatt atgccggaaa actccttcat gatgattcat aaaccatttg
     5581 gctttacggg cggtgatgcg gaggacatgc gcacctatgc cgacctgctc gataaagttg
     5641 aggcggttct gttacccgct tatgcacaga aaaccgggaa aaccaccgat gaaattgctg
     5701 ccatgctggc ggatgagacc tggatgtccg gtgccgaatg tctggcacag ggatttgctg
     5761 atcaggtgac accagccgtt aaggcaatgg catgtattca gtcaaaacgt acagaggaat
     5821 ttaaaaagat gccggaatcc attcgaaaca tgattactcc gccacgcaac agtgctccac
     5881 gcgtacagga tgatgaacct gcagcctccc ggacgccagt gcaggcagca gcacccgtgg
     5941 tggatgaaaa cagtatccgt gcgcaggtac tggcagagca aaaagcgcgt gtaaacggta
     6001 ttaatgatct gtttgccatg tttggcgggc gttatcagac gctgcaggcc cagtgtcttg
     6061 ccgatcctga atgttcgctg gagcaggccc gtgaaaagct gttgaacgag atggggcgcg
     6121 agtccacgcc atccaataaa aataccccgg ctcatattta tgccggtaac ggtaattttg
     6181 tgggggacgg gattcgccag gcgctgatgg cgcgtgccgg atttgaaaaa accgaacgtg
     6241 ataatgtcta caacgggatg accctgcgtg aatatgcccg tatgtcacta actgaacggg
     6301 gtattggggt ttccggttat aacccgatgc agatggtcgg tgcggcgttc acacacagta
     6361 cgtctgactt cggtaatatt ctgctggatg ttgcgaacaa agccattctg cagggctggg
     6421 aagatgcccc tgaaacctat gaacagtgga cgcggaaagg tcagttgtct gattttaaaa
     6481 ttgcccatcg tgtgggtatg gggggcttca gtgctctacg tcaggtgcgt gaaggggcgg
     6541 aatataaata cgtcaccacc ggagataaac aggccactat tgcactggcg acctatggcg
     6601 agctgttcag tatcacccgt caggccatta tcaatgatga tctgaatatg ctgaccgatg
     6661 tcccgatgaa actgggccgt gcggcgaaat ccactattgc cgatctggtt tatgccattc
     6721 tgacgtctaa cccgaaaatc tccacagata atgtaagtct gttcgataaa gcgaaacatg
     6781 caaacgtact ggagagcgct gcaatggacg tggcatcgct ggataaagcc cgccagttga
     6841 tgcgcgttca gaaagagggg gagcgtcatc tgaatattcg tcctgcgttc gtactggtac
     6901 cgacggcgat ggagtctgtt gctaaccagg tcattcgctc ctcaagtgtc aagggggctg
     6961 acattaacgc cggtattatt aacccggtga aagattttgc gaccgttatt gcagagcctc
     7021 gtcttgatga taacagccag accaccttct acctggctgc gtcaaaaggc tccgatacga
     7081 ttgaagtggc ttatctcaac ggtgtggata cgccatatat tgatcagatg gagggcttca
     7141 gtgtggatgg cgtgacaacg aaagtgcgta ttgacgccgg tgtcgcgcca gttgatcacc
     7201 gcggtctggt gaaatgtacg gcgtaaacgt cgcagacaac aactctgatg gcccgtaagg
     7261 gctttttttg tacctgaaat cagcccctga acggggctgt gcggagacag ttatggcaaa
     7321 gaattttgta gaagaaggaa aaacggtggc gattgttgcc agtgcagcca tcagcagcgg
     7381 agatctggtg caggtgggcg atgtttttgc ggtggcgctg accgatattc cacagggtga
     7441 aacaggcgac ggcatgaccg aaggtgtgtt tatgctgcct aagctgaaaa cggatgacat
     7501 gaaaacgggt aagaaggttt atctgaagtc cggaaaagtt cagctgacta acagcggctc
     7561 tgatccgctg gtcggggttg tctgggcaga tgccggaacc agtgcagaag aagtgccggt
     7621 aaaactcaat gtctgatccc ttttcccggc tggcagcgcg tatggatgct atcacggtta
     7681 gaaagatggg aaagacagcc tcgattaatg atgtcgatat gactgtgatc ccgggagaaa
     7741 cactggcaga gctgaatgct ctgtccggac ctgcggtctc tctggtggtg ttttcttcgg
     7801 gataccgccc acggcgcggg gatcgcgttg tttatgacgg acaacaatgg acggtcacac
     7861 ggcatgaacg ttttaacggt aagccaatga tctttattga gtaaagaggt gtgggatgaa
     7921 ggggcttgag aatgccatcc gtaatctgaa cagccttgat acccgtatgg tgccacaggc
     7981 cagcgcatgg gcgataaacc gtgtggcaca gaaagcggtc tcggtcgcca cccggcaggt
     8041 tgccgggaat accgttgcgg gagataacca ggtgaaaggg atccccctga aactggtacg
     8101 tcagcgtgtc cgggtgttta aagccagtcc gtcaggaaaa atgacggcca ggatccgcgt
     8161 taaccggggc aatctgcccg ctattaagct ggggacagcc cgggtcagac tggcccggcg
     8221 tggtggaaaa ctgcagtacc gtggcagtgt gctgaaggtg ggtaaatatc ttttccggga
     8281 tgcgtttatt cagcaactgg cgaatggtcg ctggcatgtg atgcggcgta ttgatggcaa
     8341 aaatcgttac cccattgatg tggtgaaagt cccgctgtcc ggaccgctga cacaggcatt
     8401 tgaagatgcc cgcgaccgca ttattgctgc ggaaatgccg aaacagctgg ggtatgcact
     8461 gaaacaacaa ctgaggttat ggctgacccg atgaaccgac atacacaaat ccgccaggcc
     8521 gtactggcac gccttcggga acagtgtgga gacagcgcca cgttttttga cgggcttccg
     8581 gcatttattg atgcgcagga actgcctgcc gtggcggtgt ggctgagtga tgctcagtac
     8641 accggaaaaa tgacggatga agatgactgg caggctgttc tgcatattgc tgtcttcatc
     8701 cgggcacagg caccggattc agagctggat atgtggatgg agagcaccat tttcccggcc
     8761 ctgaatgata taccggcact ttccggactc atcgacaccc tgatccctct cggttttaac
     8821 tatcaacgtg ataatgagat ggccacctgg gcgatggcgg aaatcacgta ccagatcacg
     8881 tacacgaatt aaaggaggtg gcaatgacca caccaaatcc actggcaaaa acgaaaggtg
     8941 cgggaacgac gttctggatg tacaccggca agggcgatgc gtttgcgaac cctttatcgg
     9001 acactgactg gctgcgtctt gcgatggtga aggatctgca acctggcgaa atgaccgctg
     9061 atgcagaaga tgacacttat ctcgatgatg aagatgcaga ctggaaaacg acaacccagg
     9121 ggcagaaatc cgtcggtgat acttcggcga cgctggcctg gcgtccgggt gacagcgggc
     9181 agaaaaaact ggttcagttg ttcgactccg gtgaagtctg cgcgtttcgt atcaaatatc
     9241 ccaacggcac tgttgatgtt ttccgtggct ggctgagctc actgggtaaa accattgcct
     9301 caaaagacgt gatgacccgc acagtgaaaa tcagcggtgt ggggcgtccg tatctggcag
     9361 aggaaggcac tgaaacagtg agcgttaccg ggctgacggt ggcaccggca tctgccagtg
     9421 taaaagtggg agcaaccacc acgctgacct ttacagtaaa acctgacgga gccagtgaca
     9481 aagcgatcag tgtgcattcg acagatccac agactgccac ggtgaccctg aacgggcttg
     9541 tggccacggt gaaaggcgtg aagcagggca gtgtcagcat tgtgggcatg acttctgacg
     9601 gcgattttgt ggcagtggct gcggtggctg tcagcgccgc aggttaacag gacgatactc
     9661 atcatttgcc ccggttatcc ggggcttttt tgcaggtgga gaacatgatg tttctgaaac
     9721 agggcacgtt taattatgaa aagcagtccg tggtgctcag tgagctgtcc gggctgcaga
     9781 gaattgaata tctggcgttt gttcagcagc gaacggcaaa gtttgatgcc gaagagggag
     9841 aactgccgga ggctgaacga cagattgctt ttctgcggat ggggatggat atcaatgcct
     9901 ggctggtttc ccgctcactg tggaatgcgg aacagtctca ggatgttgag acgctttgcg
     9961 catccgttat tacaacatgg tcgtatgatg ccctgggagc gggggcggag atggttctgt
    10021 cgctgagcgg tatgggagtc attgagaatg ccggggattt ggagcatgag gtgctgacgc
    10081 cggaaaagtc ctgacgcggg aaatgcagtt tgtcatgcgg cttgcccggg agttccggcg
    10141 ggcagactgg cggcggatgc tgtcggaaat gtcggccact gagcttggtg agtggggcga
    10201 ttatttccgg atgcagagct tcagtgatgt gtggatggat gcgcagtttg cctcgctgaa
    10261 ggcattgatc gtgagaatgg tgtccggtag cagtgatgct gcggtggctg atttcagcct
    10321 tttaccggaa gagaacggga taccggagcg aacggacgaa gaactgatgc atcttgggga
    10381 aggtatttcc ggaggtgtgc gttatggacc agatagccaa cttggtcatt gatttgggga
    10441 ttgatgcggc agagtttaaa aatgaaattc cccgtatcaa aaaccttctg aatggtgcag
    10501 ccagcgatgc agaacggtct tctgcccgta tgcagcgttt tatggagcgt cagactcagg
    10561 ccgcccggca gacaatgcag gcggcttctt cggctgcaac agccgcatcc gtccatgcgc
    10621 agacggtgga gaagagcgca caggctcatg aacgcatggc ccgcgaggtg gagcaaaccc
    10681 gccagcgtat ggaggcactg agccagaaaa tgcgcgagga acaggcgcag gccatggctc
    10741 tggcggaggc tcaggataaa gcggctgccg cgttttatcg tcagattgac agtgtgaaac
    10801 aggccagtgc ggggctgcag gaattacagc gtattcagca gcagatccga caggccagaa
    10861 acagtggcgg gattggtcag caggattatc tggcgctgat ttctgaggtt acggcgaaaa
    10921 cccgtgttct tacgcaggct gaggaagagg ctacccgaca gaaagtggcg tttatccgtc
    10981 agcttaaaga gcaggcaacc cgccagaatc tttcttcttc tgagttgctt cgtgctaagg
    11041 ctgcccagct gggggtaagc agtgctgcag aagtgtatat ccgcaaaatg gagcaggcag
    11101 gaaaagccac gcattcgctg ggtctgaaaa gtgcagcggc ccgccaggag attggcgttc
    11161 tgataggtga actggcccgc ggcaatttag gggcgctgag gggatccggg ataacgctgg
    11221 ctaaccgtgc cggatggata gacactctga tgtcaccgaa aggcatgatg cttggcgggg
    11281 ttattggcgg tattgccgcg tccgtctatg gtctgggtaa agcctggtat gacggtcaga
    11341 aggaggggga agaatttaac cgccagttgt cgctgacggg gcattatgcc ggagtcactg
    11401 ccgggcagct gtggacgctc agtcgtgcta tttccgggaa tggtatcacg caacatgctg
    11461 cagccggtgc gctggctcag gtggtgggga gtggtgcatt tcgtggaaac gatatcggta
    11521 tggtggcgag agctgccgca cagatggagc gatcggttgg ccagtcggtc agcgatacca
    11581 taaatcagtt taagcggctg aaggatgatc ctgtaaatgc cgcgaaggct ctggacaatg
    11641 agctgcattt tcttactgcc actcagcttg agcagatacg tgtccttggg gatcagggac
    11701 ggtccagtga tgctgcacgg atagccatgt ctgcactggc agaggaaacc ggtcggcgta
    11761 ctgcggatat tgataataac ctcaatgcgc ttggcagtac gctgaagtat ctgtctgatt
    11821 tgtggagtcg tttctgggat gcggccatga atattggtcg tgaagactcg ctggatgaac
    11881 agattgccgc tttacaggag aaagtgtcgc gggcgaaaag actcccctgg acggcatcat
    11941 cttctcaggt tgaatacgat cagcagcgtc ttaacgatct tcaggagaaa aaacgccaga
    12001 aggatttgca ggatgcaaaa gagcaggcag agcggaatta tcaggagcaa cagaaacgac
    12061 gtaatgctga aaatgctgca ctgaaccgga tgaatgaaac ggaagcagca cgacatcagc
    12121 gtgaaattgc gcgtattaat tccatgcagt acgccgatca ggctgtcagg gatgcagcga
    12181 tacaacgtga aaatgaacgt tacgagaaag ccctggcatc cggtaagaaa aaaacacgcg
    12241 aaccccgtaa tgatgaggcc acccggttat tgctgcagta cagtcagcaa caggcacagg
    12301 tggaaggaca gattgctgct gccagacagt cagcaggcat tgccacggaa aggatgacag
    12361 aagcgcataa acagcttctg gctctgcagc agcgcatcag cgacctggac gggaaaaaac
    12421 tgacggcaga tgaaaagagt gtgctggccc gtaaagatga actgattcag gcactgacgc
    12481 tgctggatgt aaaacagcag gagcttcaga aacagacggc actcaacgag ctgaagaaaa
    12541 aaacaattca gctgaccagt caactggctg aagaagagcg cgctcagcgt cagcaacatg
    12601 acctggatat cgccacggtg ggtatgggtg atcagcagcg gcagcgatat caggtacaac
    12661 tgagtcttcg ccagaaatac cagcaacagc tggagcagtt gaggcgggat agtgagcaga
    12721 aaggaacata taacacggat gactacagaa aggccgagca ggcgctgacg gagagcctga
    12781 accgacaact gaatgagaat cgccgttact ggcaacagct tgaagttgtg cagggtaact
    12841 ggaaaaacgg agtcctgcgt gcatttcagg attttaccgt ggatgcagat aatacggcag
    12901 gaacagcaga acaggtgttc tcgtcagcct tcagcaacat gggaaatggc ctggcaactt
    12961 ttgtcactac cggcaaactc aatttcaaat ccttcacctc ttctgtgctg tcagatatgg
    13021 cgaaaatcct ggcgcaggca accatgatga aatcgataaa agggattggc agtgtgctgg
    13081 gatttgatct cagcagcctt tccctgaatg ccaatggggg gatttatcag tctgctgatt
    13141 tgagtcgtta cagtggcacg gtggttaacc gtccgacgtt ttttgctttt gcaaaaggcg
    13201 cgggtgtgat gggggaagcg ggacctgaag ccattctgcc actgcgtcgt ggtgctgacg
    13261 gtaagctggg ggttgtggcg gatattggtg gttcaggtat ggcgatgttt gccccgcagt
    13321 acaacatcga gatcaataac gatggcacga acgggcagat aggtccggct gccctgaagg
    13381 tggtttatga ccttgggaaa aaagcggcag cggactttat gcaacagcag gcccgtgatg
    13441 gtggtcggtt aagtggagca tatcggtaat ggagacgttt cactggaaag tgcgcccgga
    13501 tatgaatgtg gtatcagagc cgaaagtggt gacagtgaag ctgggcgatg gttatgaaca
    13561 gcgtcgtgcg gcgggactga ataaccagtt gtcgatttac agcgtgacga tacgtgttcg
    13621 taaatgtgaa cacccatctt taaaagcctt tctggaacgg cacggtggcg tccgcgcatt
    13681 tcagtggacg ccaccttatg actggaagcc gatcagggtg gtttgtcgta aatggtcggc
    13741 aagcgtgggg gcgttgtggg taaccataac ggcagatttt gaacaggtcg tggcatagga
    13801 ggctctgatg caggatattc cacaggaaac acatcatgag acgacacgcc tcactcagtc
    13861 agcccaggtg gtgctctggg aaatcgatct gacagaggtc ggtggtgaac gttatttttt
    13921 ctgtaatgag cagaacgaaa aaggtgagcc ggttacctgg caggggcggc agtatcaggc
    13981 ataccccatt caggggacgg gatttgaact gaatggcaag ggcagtgctg cccgtccgac
    14041 actgacggtt tctaacctgc acggcatggt caccgggatg gcggaagatc tgcagagtct
    14101 ggtcggcgga acggtggtca ggcgtaaggt ttacgcccgt tttctggatg cggtgaactt
    14161 cgtcaacgga aacagtgacg ccgatccggt gcaggaggtg atcagccgct ggcgcatcga
    14221 gcagtgcagc gaactgagcg cggtgagtgc ctcttttgta ctgtccacgc cgacggaaac
    14281 ggacggtgcc gtttttccgg ggcgcatcat gctggccaac acctgcacct ggacctatcg
    14341 cggtgacgag tgcggttatc acggtccggc ggtcgcggat gaatatgatc agccgacgtc
    14401 cgatatcacg aaggataaat gcagcaaatg cctgagcggc tgtaagtttc gcaataacgt
    14461 cggcaacttt ggcggcttcc tttccattaa caaactttcg cagtgaatcc catgacagag
    14521 acagaatcag cgattctggc gcacgcccgg cgatgtgcgc cagcggagtc gtgcggcttc
    14581 gtggtgagaa caccggaggg ggaaagatat tttccctgcg tgaatatctc tggtgagccg
    14641 gaggcgtatt tccggatgtc gccggaggac tggctgagtg cagaaatgca gggagagatt
    14701 gtggcgctgg tccacagcca ccccggtggt ctgccctggc tgagtgaggc cgaccggcgg
    14761 ctgcaggtgc agagtgattt gccgtggtgg ctggtctgcc ggggggcgat tcataaattc
    14821 cgctgtgtgc cgcatctcac cgggcggcgc tttgagcacg gggtgacgga ctgttacacg
    14881 ctgttccggg acgcttacca tctggcggga attgagatgc cggattttca tcgcggggat
    14941 gactggtggc gtcacggcca gaatctctat ctggataata tggaggcaac ggggttgtat
    15001 caggtgccgt tgtcagcggc ccagccgggc gatgtgctgc tgtgctgttt tggttcatcg
    15061 gtgccgaatc atgccgccat ttactgtggc gacggcgagc tgctgcacca tattcctgaa
    15121 caactgagta aacgagagag gtacaccgac aaatggcagc gacgcacaca ctccctctgg
    15181 cgtcaccggg catggcacgc atctgccttt acggggattt gcaacgattt ggtcgccgta
    15241 tcgaccttcg tgtgaaaacg ggggctgaag ccatccgggc actggccaca cagctcccgg
    15301 tgtttcgtca gaaactgaat gagggctggt atcaggtgcg cattgccggg cgtgatgcag
    15361 gcgaaaatga attatctgcc cgtcttaatg agccgctggc aaatggtgcc gtgatccaca
    15421 tcgtgccgcg tctggcggga gctaaaagtg gcggtgtgtt tcaggtggtg cttggggcgg
    15481 cgctgattgc ggtggcatgg tggaaccctg tgggctggct gggggccgcg gctgtatcgg
    15541 gcatgtatgc ggcaggggcc agtatgatcc tgggcggagt ggcgcagatg ctggcaccga
    15601 aagccaggac gcccacggca gccagtacag ataacggcaa acagaacacc tatttctcct
    15661 cactggataa catggttgcc cagggcaatg ttctgcctgt tctgtacggt gaaatgcgcg
    15721 tggggtcacg tgtggtttct caggagatca gcacggcaga cgaaggggat ggtggtcagg
    15781 ttgtggtgat tggtcgctga tgcaaaatgt tttatgtgaa accgcctccg ggcggtttta
    15841 tcgtttatgg agcgtgagga atgggtaaag gcagcagtaa ggggcatacc ccgcgcgaag
    15901 cgaaggacaa cctgaagtcc acgcagttgc tgagtgtgat cgatgccatc agcgaagggc
    15961 cggttgaagg tccggtggat ggattaaaaa gcgtgctgct gaacagtacg ccggtgctgg
    16021 acagtgaggg gaataccaat atatccggcg tcacggtggt gttccgggcc ggtgagcagg
    16081 agcagacacc gccggaggga tttgaatcct ccggctccga gacggtgctc ggtacagaag
    16141 tgaaatatga cacgccgatc acccgcgcca tcacgtcggc aaacatcgac cgactgcgct
    16201 ttaccttcgg tgtgcaggca ctggtggaaa ccacctcaaa gggggaccgg aatccgtcgg
    16261 aagttcgcct gctggttcag atacagcgta atggtggctg ggtgacggaa aaagacatca
    16321 ccattaaggg caaaaccacc tcgcagtatc tggcctcggt ggtggtggat aacctgccgc
    16381 cgcgcccgtt taatatccgg atgcgcagaa tgacgccgga cagcaccaca gaccagctgc
    16441 agaacaaaac gctctggtcg tcatacaccg aaatcatcga tgtgaaacag ggctacccga
    16501 acacggcact ggtcggcgta aaggtggatt cggagcagtt cggcagccag caggtgagcc
    16561 gtaattatca tcttcgcggg cgtattctgc aggtgccgtc gaactataac ccgcagacgc
    16621 ggcaatacag cggtatctgg gacggaacgt taaaaccggc atacagcaac aacccggcct
    16681 ggtgtctgtg ggatatgctg acccacccgc gctacggcat ggggaaacgt cttggtgcgg
    16741 cggatgtgga taaatgggcg ctgtatgtca tcggccagta ctgcgaccag tcggtgccgg
    16801 acggctttgg cggcacggag ccgcgcatta cctgtaatgc gtacctgacc acacagcgta
    16861 aggcgtggga tgtgctcagt gatttctgct cggcgatgcg ctgtatgccg gtatggaacg
    16921 ggcagacgct gacgttcgtg caggaccgac catcagataa ggtgtggacc tataaccgca
    16981 gtaatgtggt gatgccggat gatggtgcgc cgttccgcta cagcttcagc gccctgaagg
    17041 accgccataa tgccgttgag gtgaactgga ttgacccgaa taacggctgg gagacggcga
    17101 cagagcttgt tgaagatacg caggccattg cccgttacgg tcgtaatgtc acgaagatgg
    17161 atgcctttgg ttgtaccagt cgggggcagg cacaccgagc cgggctgtgg ctgattaaaa
    17221 cggaactgct ggaaacgcag accgtggatt tcagcgtggg cgcagaaggg cttcgccatg
    17281 tgccgggcga tgtcattgaa atctgcgatg atgactatgc cggtatcagc atcggtggtc
    17341 gcgtgctggc ggtgaacagc cagacccgga cgctgacgct cgaccgtgaa atcacgctgc
    17401 catcctccgg caccacgctg ataagcctgg ttgacgggca gggtaatccg gtcagcgtgg
    17461 aggtccagtc cgtcaccgac ggcgtgaagg tgaaagtgag ccgtgttcct gacggcgttg
    17521 ctgaatacag cgtatggggg ctgaagttgc cgacgttgcg ccagcgcctg ttccgctgcg
    17581 tgagtatccg tgagaacgac gacggcacgt atgccatcac cgccgtgcag catgtacccg
    17641 aaaaagaagc catcgtggat aacggggcgc actttgacgg cgaccagagc ggcacggtga
    17701 atggtgtcac gccgcccgcg gtgcagcacc tgactgccga agtcaccgca gacagcgggg
    17761 aatatcaggt gctggcgcgc tgggacacgc cgaaggtggt gaagggcgtg agctttatgc
    17821 ttcgcctgac cgtggcagcg gatgacggca gtgagcggct ggtcagcacg gcccggacga
    17881 cggaaaccac ataccgcttc acgcaactgg cgctggggaa ctacaggctg acagtccggg
    17941 cggcaaatgc ctgggggcag cagggcgatc cggcgtcggt atcgttccgg attgccgcac
    18001 cggcagcacc gtcgcggatt gagctgacgc cgggctattt tcagataacc gcaacgccgc
    18061 atcttgccgt ttatgatccg acggtacagt ttgagttctg gttctcggaa aagcggatta
    18121 ccgatatcag gcaggttgaa accacagccc gctaccttgg cacggggctg tactggatag
    18181 ccgccagtat caatatcaaa ccgggccatg attattactt ttatatccgc agtgtgaaca
    18241 ccgttggcaa atcggcattc gtggaggccg tcggtcgggc gagcgatgat gcggaaggtt
    18301 acctggattt tttcaaaggc aagataaccg aatcccatct cggtaaagag ctgctggaaa
    18361 aagttgatct gacggaggat aacgccagca gactggatga gttttcgaaa gagtggaagg
    18421 acgctaacga taaatggaat gccatgtggg gcgtcaaaat tgagcagacc aaagacggca
    18481 aacattatgt cgcgggtatt ggtctcagca tggaggacac ggaggaaggc aagctgagcc
    18541 agtttctggt tgccgctaac cgtatcgcgt ttattgaccc ggcaaacggg aatgaaacgc
    18601 cgatgtttgt ggcgcagggc aaccagatat tcatgaacga cgtgttcctg aagcgcctga
    18661 aagcccccac cattaccagc ggtggcagcc ctccggtatt ttccctgaca tcagacggaa
    18721 agctgaccgc taaaaatgcg gatatcagtg gcagtgtgaa tgcgaactca gggacgctca
    18781 acaacgtcac gattaatgag aactgtcaga ttaaggggaa actgtcagcc aaccagattg
    18841 aaggtgatat tgtcaaaacg gtcagcaagt ctttcccccg cacgagca
//
LOCUS       DAJOWB010000089        18452 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJOWB010000089 DAJOWB010000000
VERSION     DAJOWB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.089"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(31..1296)
                     /locus_tag="OHO85_003351"
                     /pseudo
     CDS             complement(31..1296)
                     /locus_tag="OHO85_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
     gene            complement(1482..1566)
                     /locus_tag="OHO85_003352"
     tRNA            complement(1482..1566)
                     /locus_tag="OHO85_003352"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1530..1532),aa:Leu,seq:caa)
     gene            1762..2781
                     /gene="ahr"
                     /locus_tag="OHO85_003353"
     CDS             1762..2781
                     /gene="ahr"
                     /locus_tag="OHO85_003353"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCQ2672689.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADY"
     gene            complement(2785..3348)
                     /gene="idnK"
                     /locus_tag="OHO85_003354"
     CDS             complement(2785..3348)
                     /gene="idnK"
                     /locus_tag="OHO85_003354"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCQ2672690.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            3565..4596
                     /gene="idnD"
                     /locus_tag="OHO85_003355"
     CDS             3565..4596
                     /gene="idnD"
                     /locus_tag="OHO85_003355"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HCQ2672691.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            4620..5384
                     /gene="idnO"
                     /locus_tag="OHO85_003356"
     CDS             4620..5384
                     /gene="idnO"
                     /locus_tag="OHO85_003356"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCQ2672692.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            5447..6766
                     /gene="idnT"
                     /locus_tag="OHO85_003357"
     CDS             5447..6766
                     /gene="idnT"
                     /locus_tag="OHO85_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HCQ2672693.1"
                     /translation="MPLIIIAAGVALLLILMIVFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQILVDSGVGHYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6833..7831
                     /gene="idnR"
                     /locus_tag="OHO85_003358"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6833..7831
                     /gene="idnR"
                     /locus_tag="OHO85_003358"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HCQ2672694.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7909..9411
                     /locus_tag="OHO85_003359"
     CDS             7909..9411
                     /locus_tag="OHO85_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCQ2672695.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAQAGTVSEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATISDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(9572..10654)
                     /gene="lptG"
                     /locus_tag="OHO85_003360"
     CDS             complement(9572..10654)
                     /gene="lptG"
                     /locus_tag="OHO85_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCQ2672696.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(10654..11754)
                     /gene="lptF"
                     /locus_tag="OHO85_003361"
     CDS             complement(10654..11754)
                     /gene="lptF"
                     /locus_tag="OHO85_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCQ2672697.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            12021..13532
                     /gene="pepA"
                     /locus_tag="OHO85_003362"
     CDS             12021..13532
                     /gene="pepA"
                     /locus_tag="OHO85_003362"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCQ2672698.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            13886..14329
                     /gene="holC"
                     /locus_tag="OHO85_003363"
     CDS             13886..14329
                     /gene="holC"
                     /locus_tag="OHO85_003363"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCQ2672699.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            14329..17184
                     /gene="valS"
                     /locus_tag="OHO85_003364"
     CDS             14329..17184
                     /gene="valS"
                     /locus_tag="OHO85_003364"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCQ2672700.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADNKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(17240..18436)
                     /locus_tag="OHO85_003365"
     CDS             complement(17240..18436)
                     /locus_tag="OHO85_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCQ2672701.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGAV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
BASE COUNT         4706 a         4600 c         4700 g         4446 t
ORIGIN      
        1 gaataattcc cgggcatttg cccgggatga ttactgtttt aatggattat taatctttgc
       61 atattcaaat ggactgataa acctttctct gtttacatcc agaaaatcgg cccaccactg
      121 tagcatcaat cgccgttctt ccagatgctc tgctttatgg atatacgcgg ccctcactga
      181 atttcgcgcc atgtggctca tctgacgttc aacagcatca cgagaccaca gacctgattc
      241 gaccaatgaa ctacaggcca ttgttcgaaa gccatgacca caaacctcta cttttgtatc
      301 ataccccatg acccgtaacg cactatttac cgtattctca ctcatgggtt tgtgcgaatc
      361 gtgatcacca ataaatatca agtcatgggc cccataaaac tgttttatct gctttaaaat
      421 tgcaagagct tgcgttgaaa gaggcactag atgcgttgta cgcatttttg agcctctatg
      481 ggaatgtttc actccaggaa taggctcccg ctccggtggg atagtccata tagacgcttc
      541 gaaatcgatc tctgaccaac gagcaaaacg cagctcactg gaccgaataa agatcagcaa
      601 agtgagttct atcgcccatc gggttagcgg cctaccagta tagctatcta tttttgtaag
      661 caactcaggg atgcgcttta attcaagcgc gggacgatgt tgtcgattac aggaagcaac
      721 cgccccagcc atctcttgtg ccgggttata atcaattaac ccactttgca ctgcatagcg
      781 catgatggct gtagtgcgct gctgaagacg agcggccact tcaagacgtc cagacatttc
      841 tacggcctta ataggtgcta ataaatctcg agtttttaac tcagcgatat tacgttcacc
      901 aagcgctgca aaaagattat cttcaagact ttttagcaca cgatgggcgt gatcttcaga
      961 ccacttttta ttggtgccat gccactcaat cgcgacttct ttaaaggttc gtgctttact
     1021 ctgttcaacc ttatcatttt tctttttgtc tcccggatcg acgccattcg caagcagctt
     1081 acgcgtctcg tcacgacgta ctctggcatc cgctagtgtg atttcaggat aaaccccaag
     1141 tgccagcatt ttttgctttc cctcataacg gtactgcaaa cgccagtact tagaaccatt
     1201 tggatggaca agcagatgca taccgccacc atcagtcagc taatattgct tatccgttgg
     1261 cttggctgct ctgactttga tatctgttaa tgccatcgta ttccctccct gttggtacaa
     1321 gcattatcga accggaaata ccatcatctg taccaacata tgttggtaga tgtacgttga
     1381 tatcggttga ccttgagaga gttaatatag cgggaaagat agataaacac tggatttcag
     1441 gcataaaaaa agacctcagt tgaggtctat ttacatactt ttggtgccga aggccggact
     1501 cgaaccggca cgtatttcta cggttgattt tgaatcaact gcgtctaccg atttcgccac
     1561 ttcggcactg aagaggtatg cggaaaacgt tgtggattat acctgtcgca cgccaccatg
     1621 caagcgccag cttgctcgca cctcgctaag tgctgaaatt ttcagcattg catacagcga
     1681 tgtgtaacct ttgtcacact ccaggcaccc cgccctgcca tgctctacac ttcccaaaca
     1741 acaccagaga aggaccaaaa aatgtcgatg ataaaaagct atgccgcaaa agaagcgggc
     1801 ggcgaactgg aagtttatga gtacgatccc ggtgagctga ggccacaaga tgttgaagtg
     1861 caggtggatt actgcgggat ctgccattcc gatctgtcga tgatcgataa cgaatgggga
     1921 ttttcacaat atccgctggt tgccgggcat gaggtgattg ggcgcgtggt ggcactcggg
     1981 agcgccgcgc aggataaagg tttgcaggtc ggtcagcgtg tcgggattgg ctggacggcg
     2041 cgtagctgtg gtcactgcga cgcctgtatt agcggtaatc agatcaactg cgagcaaggt
     2101 gcggtgccga cgattatgaa tcgcggtggc tttgccgaga agttgcgtgc ggactggcaa
     2161 tgggtgattc cactgccaga aaatattgat atcgagtccg ccgggccgct gttgtgcggc
     2221 ggtatcacgg tctttaaacc actgttgatg caccatatca ctgctaccag ccgcgttggg
     2281 gtaattggta ttggcgggct ggggcatatc gctataaaac ttctgcacgc aatgggatgc
     2341 gaggtgacag cctttagttc taatccggcg aaagagcagg aagtgctggc gatgggtgcc
     2401 gataaagtgg tgaatagccg cgatccgcag gcactgaaag cactggcggg gcagtttgat
     2461 ctcattatca acaccgtcaa cgtcagcctc gactggcagc cctattttga ggcgctgacc
     2521 tatggcggta atttccatac ggtcggtgcg gttctcacgc cgctgtctgt tccggccttt
     2581 acgttaattg cgggcgatcg cagcgtctct ggttctgcta ccggcacgcc ttatgagctg
     2641 cgtaagctga tgcgttttgc cgcccgcagc aaggttgcgc cgaccaccga actgttcccg
     2701 atgtcgaaaa ttaacgacgc catccagcat gtgcgcgacg gtaaggcgcg ttaccgcgtg
     2761 gtgttgaaag ccgattattg aaaatcattc gcagcgctga tctgaggcgc tgccctcttt
     2821 cgcacatatt ctgttttgtc gtatcgccag cacagcctgc cgacattgtt cggtgacatt
     2881 ggcaatatca tggttgatat caatgcgcac aatatcctgt tcatctgctt gtggacgctc
     2941 cagtgcctca aactgacttt ttagtaacgc taccggcata aaatgcccag cccgacgctg
     3001 cattcgcgcg agaatagttt catagtcgcc atctaaccag aggaaatgaa catgggggct
     3061 acccttgcgt aaaatatcac gatactgttt ttttaatgat gaacagacaa taaatcctgt
     3121 ttcattcttt ttataaagac tgtatgaagc atcatttaag cgttccagcc agggaagtcg
     3181 atcttcatca gataatggaa taccctgcga cattttatct atatttttgg ctggatgaag
     3241 atcgtcacca tcaataaatt tagcagataa taacgcggca accttgctac caattaatgt
     3301 tttaccactc cctgaaacgc ccatcaaaat aaagctttca cccgccattt aaaatctcct
     3361 gaatcatctt acctgctgcg atactacgcc tggcggcata ataatttcac gtttttttat
     3421 ttctactgat aagaattaca aggcacatca cgttatgcgt aacatagtaa tgtaacaatt
     3481 ttctgacgtg atcttcatca caaataatga cagttaaaac cgcttaaatg cttccgaggt
     3541 gtctacctga ccagtgagga atttatgcaa gtgaaaacac agtcctgcgt tgttgcgggc
     3601 aagaaaactg ttgccgttac cgagcagacg atagattgga ataataatgg aacattagta
     3661 caaataaccc gaggtggaat ttgcggttcc gatttacatt attatcagga aggaaaagta
     3721 ggtaatttca tgataaaggc accgatggtg ttaggtcatg aagttatcgg taaagttatt
     3781 catagcgact catcagaatt acatgaaggg caaacggtag ccattaatcc gtctaaaccg
     3841 tgcggtcact gcaaatactg cattgaacat aacgagaatc agtgtacaga tatgcgtttt
     3901 tttggcagtg ccatgtattt ccctcatgtt gatggtggtt ttacccgtta taaaatggtc
     3961 gaaacgtcgc aatgtgtccc ttatccggcc aaagctgacg aaaaggttat ggcttttgcc
     4021 gaacctttag ccgtcgcgat tcatgccgca catcaggccg gcgagttaca gggcaagcga
     4081 gtatttattt ccggtgttgg acccattggc tgcctgattg tcagtgcagt gaaaacactg
     4141 ggggccgcgg aaattgtctg tgctgatgtg agtccccgtt ccctttcgct gggcaaagag
     4201 atgggggcgg atgtgctcgt aaacccacaa aacgacgaca tggatcactg gaaagcggaa
     4261 aaaggctatt tcgatgtcag ctttgaagtg tccggtcatc cttcatcagt gaatacctgt
     4321 ctggaggtca ctcgtgcacg cggcgtaatg gtgcaggtag gtatgggagg cgcgatggca
     4381 gaattcccaa tgatgacgtt gattggtaag gagatttcac tcagaggctc tttccgtttt
     4441 accagcgaat ttaataccgc agtgtcatgg ctggcgaatg gcgttatcaa tccactgcct
     4501 ttactgagtg ctgaatatcc cttcactgac ctggaagagg cgctacgttt cgccggtgat
     4561 aaaacccagg cagcaaaagt ccagcttgtt ttctaagaga taaataaagg aataatacaa
     4621 tgaacgatct attttcactg gcaggaaaaa atatcttgat taccggttca gcacagggca
     4681 ttggcttttt actggcaacc ggcctgggta aatatggcgc acaaataatt attaatgata
     4741 ttactgccga acgcgcagaa cttgctgtag aaaaactcca ccaggagggt attcaggccg
     4801 ttgccgcacc ttttaatgtt actcataaac atgaaattga tgccgccgtt gaacatatcg
     4861 aaaaggacat cggccccatt gatgtgctgg tgaataacgc cggtatccag cgccgtcatc
     4921 cttttactga gttccctgaa caagagtgga atgatgtgat cgcagtaaac cagaccgccg
     4981 tgttcctggt atcgcaagcg gtaactcgtc acatggttga acgcaaggca ggtaaagtta
     5041 ttaatatttg ctcgatgcaa agcgaactgg gacgtgacac catcacccct tatgccgcat
     5101 cgaaaggggc ggtaaaaatg ctcacccgcg gcatgtgcgt cgagctggcg cgccacaata
     5161 ttcaggtcaa cggtattgcg ccgggctatt tcaaaacaga aatgactaaa gcactggttg
     5221 aggacgaagc cttcaccgcc tggttgtgca aacggacccc cgccgcacgc tggggagatc
     5281 cgcaggaact gattggtgct gcggtgttcc tttcttcaaa agcctctgat ttcgtaaacg
     5341 gccacctgtt gtttgttgat ggcggcatgt tagtggctgt ttaacagccc cccctacgtg
     5401 cagcgcgaaa gcgctgcttt tctggcacta ataagagata aagactatgc cattaatcat
     5461 tattgcggca ggcgtcgcgc tgcttcttat cctgatgatc gtctttaaag ttaacggctt
     5521 tattgccctc gttctggtag ctgccgtcgt cggatttgcc gaagggatgg atgcacaggc
     5581 cgtcctgcac tctatacaaa atggtatcgg cagcacgctc ggcgggctgg caatgatcct
     5641 cggtttcggg gccatgttag gcaagctgat ttctgatacg ggtgcggcac aacgtatcgc
     5701 cactacgctg attgctactt ttggtaaaaa acgcgtgcaa tgggcgctag tgatcaccgg
     5761 tctggttgtg ggcctcgcca tgttttttga agtgggtttt gtcctgctgt tgccgttggt
     5821 atttaccatc gtagcatcat caggattacc cctgttgtat gttggcgtac caatggtagc
     5881 agcgctctct gtaacccact gttttctgcc gccacatcca gggcctactg ccatcgcgac
     5941 tatctttgag gctaatctcg gaacgacttt actgtatgga tttatcatta ccattccgac
     6001 agttattgtc gcaggaccgc tgttttctaa actgctaact cgctttgaga aagcaccacc
     6061 ggaaggctta tttaatcctc atctgtttag cgaagaggag atgccctcct tctggaacag
     6121 tattttcgct gccgtgatcc cggtcatcct gatggctatc gccgccgttt gtgaaattac
     6181 gttaccgaaa actaacaccg tgcgcctctt ctttgaattt gtcggtaacc ctgccgttgc
     6241 gctgtttatt gccattgtta ttgcgatttt cacactgggc cgacgtaatg gacgcaccat
     6301 cgagcaaatc atggatatca ttggggattc tataggcgct atcgcgatga ttgtgtttat
     6361 tatcgctggc ggcggcgcgt ttaagcagat attagtagat agcggtgtcg ggcactatat
     6421 ttcacactta atgaccggaa ctacactttc gccgttattg atgtgctgga ctgttgcggc
     6481 gctgttgcgt atcgctctgg gctctgccac cgtcgcggcc attaccaccg cgggtgtggt
     6541 gttgccgatt atcaacgtta cccatgccga tcccgcttta atggtactgg caaccggtgc
     6601 gggcagcgtg atcgcgtcac acgtaaacga ccctggcttc tggctattta aagggtattt
     6661 taatctgacg gttggtgaaa cgttgcgtac ctggacggtg atggaaaccc ttatttctat
     6721 tatgggtttg ctgggcgtgt tagccattaa cgccgtattg cactgaaaga ataaaatatt
     6781 gtgatgtagt ttcgggggaa gattgaagat tcccccgacg ttgggagaag ttatgcgcaa
     6841 tcacagaatt tctttacagg atatcgctac gctggctggc gtaacaaaaa tgaccgtgag
     6901 tcgttatatc cgctcgccga aaaaggtggc aaaggaaaca ggcgagcgca tcgccaaaat
     6961 tatggaagag atcaattata tccctaatcg cgctcccggc atgttgttga atgcacaaag
     7021 ttataccctc ggtatcttga ttccgtcatt tcaaaatcaa ctcttcgctg acattctcgc
     7081 gggaatagaa tccgtcacct ctgagcataa ttatcagacc cttattgcta attacaatta
     7141 cgatcgcgat tccgaagaag agtcggtgat taacctgctc tcttataaca ttgacgggat
     7201 tatactttcg gaaaaatatc acaccatcag gacggtgaaa tttctgcgtt cagccaccat
     7261 tccggtcgtg gaattgatgg atgtacaggg agaacggctg gatatggagg tcggttttga
     7321 taatcggcag gccgcttttg acatggtgtg taccatgctg gagaagcgag tcaggcacaa
     7381 aattctgtat ctcggttcca aagatgacac ccgcgatgaa cagcgttatc aggggtattg
     7441 cgatgcgatg atgctgcata atctttcccc attacgcatg aatccacgcg ccatctcatc
     7501 cattcattta ggaatgcagt taatgcgtga tgcccttagc gcaaacccgg atttagacgg
     7561 tgtattttgc accaatgacg atattgcgat gggtgcgctt ctgctgtgcc gcgagcgaaa
     7621 cctcgccgtg ccagaacaaa tttccattgc gggttttcat gggctggaga ttggcaggca
     7681 aatgatccca agcctcgcca gcgtaattac tccccgtttt gatattggtc ggatggccgc
     7741 ccaaatgttg ctgagcaaaa ttaaaaataa cgatcacaac cacaacactg ttgacctcgg
     7801 gtatcagatt tatcacggca acacgcttta ggttattttc tcttcattac acgtattctc
     7861 tttgccggac gttgcctata ctccaggcag gaaactggag gatatctcat gagtgaaccc
     7921 ctgttaattg cccgcacgcc ggacacagaa ctgtttttac tgccgggaat ggctaaccgt
     7981 catgggctga ttaccggcgc aacggggacg ggtaaaaccg ttacgctgca aaaactggcg
     8041 gagtcattgt cggaaatcgg cgtgccggtg tttatggctg atgtgaaagg cgatctgacc
     8101 ggcgtcgcgc aggcaggaac ggtgtcggaa aaactgctcg caaggcttaa aaatatcggc
     8161 gtcaatgact ggcaaccgca tgccaatccg gtggtggtgt gggatatctt tggcgagaaa
     8221 ggccatccgg tgcgggcgac gatttcggat ctggggccgc tgttgctggc acgactgttg
     8281 aatctcaacg atgtgcaatc tggcgtgctg aatatcatct tccgcattgc tgacgatcag
     8341 gggctgttac tgctcgactt taaagatctg cgggcaatta cccagtacat cggcgataac
     8401 gccaaatcct tccagaatca gtacggtaat atcagtagcg catcggttgg tgccatccag
     8461 cgcgggctgt tgtcgctgga acagcaaggc gcagcacact tctttggtga gccgatgctg
     8521 gatatcaaag actggatgcg caccgatgcc aacggtaaag gcgttatcaa tatcctcagc
     8581 gccgagaaac tttatcagat gccgaaactg tacgccgcca gcctgctgtg gatgctttca
     8641 gagttgtatg aacaattgcc ggaagcgggc gatctggaga aaccaaaact ggtgtttttc
     8701 ttcgacgagg cacatctgct gtttaacgat gccccgcagg tactgctgga taagattgag
     8761 caggtgataa ggcttattcg ctcaaaaggc gtgggcgtct ggttcgtttc gcaaaacccg
     8821 tctgatattc cggataatgt gctcgggcag ctcggtaatc gcgttcaaca tgctttgcgg
     8881 gcttttacgc caaaagatca gaaagcagtg aaggcagcgg cgcaaaccat gcgggccaat
     8941 ccggcgtttg ataccgaaaa ggcgattcag gaactgggca ccggcgaggc gttgatctct
     9001 tttctggatg cgaaaggaag cccttctgtg gtggagcgtg cgatggtgat cgcgccttgt
     9061 tcgcggatgg ggccggtgac ggaagatgag cgtaatggct tgattaatca ctctccggtg
     9121 tatggcaaat atgaggatga ggtggaccgg gaatccgcct atgagatgtt gcaaaaaggc
     9181 tttcaggcca gtaccgagca gcaaaataat cctcccgcga aagggaaaga ggtagcggtg
     9241 gatgacggca ttcttggtgg attgaaggat attttgttcg gcactaccgg accacgcggc
     9301 gggaagaaag atggtgtggt gcaaacaatg gccaaaagcg ccgctcgcca agtgacgaat
     9361 cagattgtac gcgggatgtt ggggagtttg ctggggggga gaagaaggta agtcaggcga
     9421 gatttgtctt tgcctgatgc gacgctggcg cgtcttatca tgcccacccc actgcaatat
     9481 attgaatttt aattattttt caggaatgaa cgaagcacac tttgctaaca ataataaagg
     9541 gagctttcgc tccctttatt cgttcattcg gttacgattt tctcattaac agccacaggc
     9601 tgattaagaa gaagctggcg cttggcaaca gtgcgccgat gatcggcggg atgccataaa
     9661 ccaacgtcag cgggccgaag atctggtcca gtacgtagaa gacaaaaccg aaactgatac
     9721 cggtgaccac acgcacgccc atcggtacgc tacgcagtgg gccaaagatg aacgacagcg
     9781 ccatcagcat catcaccgcc acagatagcg gctggaagat tttgctccac atgttgagct
     9841 gataacgtcc ggcatcctga ccgctcgact tcagatactt cacatagttg tgcaaaccgc
     9901 tgatagagag tgcatccggg tccagcgcca ccacgcccag tttgtccggc gtgaggttgg
     9961 ttttccaggt gccgctcacc gtctgcgaac cggtaatctg tttcggattg gtcagatcag
    10021 attcatcaac ctgcgacaga cgccagactt tatgttccgg gtcaaacttc gcagtagcgg
    10081 catagcgtac ggattgcaga cgacgattct cgttaaaggc ataaatgctg atgccaccta
    10141 actcttcgtc acctttaacc cgctcaatgt agacgaagtt gttgccatct ttcgcccata
    10201 agccttgctg ggtagagagc aacgagccgc cgtacatcgc ctgcgcacgg tagttacgcg
    10261 ccatctgctc gccctgcggc gcgacccatt cgccaatcgc catcgtcagc aagaccagcg
    10321 gaatggcggt tttcatcacc gacagcgcca cctgcatacg ggtaaaacca gaagcctgca
    10381 tcaccaccag ttcgctgcgc tgcgccagca tcccaagacc aagcaacgcc ccaagcagag
    10441 ccgccatcgg gaagaagatc tgcacatctt tcggcacgct cagcaaggta tacattcctg
    10501 cgcctaacgc gtcgtaactc ccctgcccgg cttttttcag ctgatcgaca aacttgataa
    10561 tgcccgacag cgacaccagc atgaacagtg tcatcatgat ggtggtgaaa atagttttac
    10621 cgatatagcg gtcaagtacg ccaaaaggtt gcatcacacc gctcctttac gcgaaaaact
    10681 ggcgcgcagg cggcggaccg gcacggtgtc ccaaaggttg agaacaatcg ctaaagccag
    10741 ataaatcagg ttaacggtcc acatccacag cgtcgggtcc agcttacctt taccgccgtt
    10801 cgatttcagg gaggtctgga tcaggaagaa aagtagatac agcagcatgg ctggcagcat
    10861 cgacagtacg cgtccctgac gtgggttaac cacgctcagc ggtacgacca taagtgccat
    10921 cataaacacg gtgaatacca acgtgatacg ccagttcagt tctgcgcgag cacgatcggt
    10981 gtcagtgttc cacaatgtgc gcatgtccat ctggtcggta tcgttcgggt cgagcgccac
    11041 cgcctggtga ccaatgatcg cctgataatc ctggaagtcc gtaatgcgga aatcacgtaa
    11101 caatgcagtg ccttcgaagc gcgttccctg gttgagagtg acgacctggg agccgtcgcg
    11161 cagctgggtt aaatgtccgg aatcggccac caccacagaa ggacgtgcat taccttttgg
    11221 tcgaatttgc gcgaggaaca catctttgaa atcgctgccg tcaacgcttt cgatgaacag
    11281 caccgagctg ccattagtcg cttgctggaa ttgcccttgc gccagcgccg ccatgccagg
    11341 gttcgctttc gcttctgcta acacttcatc ctgatgacgc gatgaccacg gtcccgccca
    11401 catcacgtta accgccgcta cgattgccgt gaataccgca aggatcattg ccgctttcac
    11461 cagaaccgct ttgctcaggc cgcaggcatg cattaccgta atttcacttt cggtatacag
    11521 tttgcccagc gtcatcagca gcccgaggaa caggcttaat ggcaggataa gctgcgccat
    11581 ttccggcacg cccaacccga gaagggagag caccagattc gccggaatat cgccgtcaac
    11641 cgctgcgccg aggatcctca ctaacttttg acagaagaag atcaaaagca agatgaagag
    11701 tatcgccagc tggcttttga gcgtctcccg caccagatat cttatgatta tcactttaaa
    11761 tacgcccgta aaaactcgtc ttttgcagga ttttagcttg tttcatggct taaacgtcat
    11821 ttattctctt gagtcgtcga aatcgtcgct aagataatta tactcaacgg attcacctct
    11881 cagattttgt tctgacgtgc caatgccgta ataacgttaa gattaacacg aagtcatcgc
    11941 aacagcggac atgagttacg aaagcttgca attctatctg tagccaccgc cgttgtcttt
    12001 aagattcagg agcgtagtgc atggagttta gtgtaaaaag cggtagcccg gagaaacagc
    12061 ggagtgcctg catcgtcgtg ggcgtcttcg aaccacgtcg cctttctccg attgcagaac
    12121 agctcgataa aatcagcgat gggtacatca gcgccctgct acgtcggggc gaactggaag
    12181 gaaaaccggg gcagacattg ttgctgcacc atgttccgaa tgtactttcc gagcgaattc
    12241 tccttattgg ttgcggcaaa gaacgtgagc tggatgagcg tcagtacaag caggttattc
    12301 agaaaaccat taatacgctg aatgatactg gctcaatgga agcggtctgc tttctgactg
    12361 aactgcacgt taaaggccgt aacaactact ggaaagtgcg tcaggctgtc gagacggcaa
    12421 aagaaacgct ctacagtttc gatcagctga aaacgaacaa gagcgaaccg cgtcgtccgc
    12481 tgcgtaaaat ggtgttcaac gtgccgaccc gccgtgaact gaccagcggt gagcgcgcga
    12541 tccagcacgg tctggcgatt gccgccggga ttaaagcagc aaaggatctt ggcaatatgc
    12601 cgccgaatat ctgtaacgcc gcttacctcg cttcacaagc gcgccagctg gctgacagct
    12661 acagcaagaa tgttatcacc cgcgttatcg gcgaacagca gatgaaagag ctggggatgc
    12721 attcctatct ggcggtcggt cagggttcgc agaacgaatc gctgatgtcg gtgattgagt
    12781 acaaaggcaa cgcgtcggaa gatgcacgcc caatcgtgct ggtgggtaaa ggtttaacct
    12841 tcgactccgg cggtatctcg atcaagcctt cagaaggcat ggatgagatg aagtacgata
    12901 tgtgcggtgc ggcagcggtt tacggtgtga tgcgtatggt cgcggagcta caactgccga
    12961 ttaacgttat cggcgtgttg gcaggctgcg aaaacatgcc tggcggacga gcctatcgtc
    13021 cgggcgatgt gttaaccacc atgtccggtc aaaccgttga agtgctgaat actgacgctg
    13081 aaggccgcct ggtactgtgc gacgtgttaa cttacgttga gcgttttgag ccggaagcgg
    13141 tgattgacgt ggcgacgctg accggtgcct gcgtgatcgc gctgggtcat cacattaccg
    13201 gtctgatggc gaaccataat ccgctggccc atgaactgat tgccgcgtct gaacaatccg
    13261 gtgaccgcgc atggcgctta ccgctgggtg acgagtatca ggaacagctg gagtccaatt
    13321 ttgccgatat ggcgaacatt ggcggtcgtc ctggtggggc gattaccgca ggttgcttcc
    13381 tgtcgcgctt tacccgtaag tacaactggg cgcacctgga tatcgccgga accgcctggc
    13441 gttctggtaa agcaaaaggc gcaaccggtc gtccggtagc gttgctggca cagttcctgt
    13501 tgaaccgcgc tgggtttaac ggcgaagagt aattgcgtca ggcaaggctg ttattgccgg
    13561 atgcggcgtg aacgccttat ccgacctaca cagcactgaa ctcgtaggcc tgataagacg
    13621 cgcaagcgtc gcatcaggca atgctgttat tgtcggatgc ggcatgaacg ccttatccga
    13681 cctacacagc accgaactcg taggcctgat aagacgcggc agcgtcgcat caggcaatgc
    13741 tgttattgtc ggatgcggcg taagcgcctt atccgaccta cacacagcac tgaactcgta
    13801 ggcctgataa gacacaacag cgtcgcatca ggcgctgcgg tgtatacctg atgcgtattt
    13861 aaatccacca caagaagccc catatatgaa aaacgcgacg ttctaccttc tggacaatga
    13921 caccaccgtc gatggcttaa gcgccgttga gcaactggtg tgtgaaattg ccgcagaacg
    13981 ttggcgcagc ggtaagcgcg tgctcatcgc ctgtgaagat gaaaagcagg cttaccggct
    14041 ggatgaagcc ctgtgggcgc gtccggcaga aagctttgtt ccgcataatt tagcgggaga
    14101 aggaccgcgc ggcggtgcgc cggtggagat cgcctggccg caaaaacgca gcagcagccc
    14161 gcgcgatata ttgattagtc tgcgaacaag ctttgcagat tttgccaccg ctttcacaga
    14221 agtggtagac ttcgttcctt atgaagattc tctgaaacaa ctggcgcgcg aacgctataa
    14281 agcctaccgc gtggctggtt tcaacctgaa tacggcaacc tggaaataat ggaaaagaca
    14341 tataacccac aagatatcga acagccgctt tacgagcact gggaaaagca gggctacttt
    14401 aagcctaatg gcgatgaaag ccaggaaagt ttctgcatca tgatcccgcc gccgaacgtc
    14461 accggcagtt tgcatatggg tcacgccttc cagcaaacca tcatggatac catgatccgc
    14521 tatcagcgca tgcagggcaa aaacaccctg tggcaggtcg gtactgacca cgccgggatc
    14581 gctacccaga tggtcgttga gcgcaagatt gccgcagaag aaggtaaaac ccgtcacgac
    14641 tacggccgcg aagctttcat cgacaaaatc tgggaatgga aagcggagtc cggcggcacc
    14701 attacccgtc agatgcgccg tctcggcaac tccgtcgact gggagcgtga acgcttcacc
    14761 atggacgaag gcctgtccaa tgcggtgaaa gaagttttcg ttcgtctgta taaagaagac
    14821 ctgatttacc gtggcaaacg cctggtaaac tgggacccga aactgcgcac cgctatctct
    14881 gacctggaag tggaaaaccg cgaatcaaaa ggttcgatgt ggcacatccg ctatccgctg
    14941 gctgacggcg cgaaaaccgc agacggtaaa gattatctgg tggttgcgac tacccgtccg
    15001 gaaaccctgc tgggcgatac tggcgtagcc gttaacccgg aagatccgcg ttacaaagat
    15061 ctgattggca aatatgtcat tctgccgctg gttaaccgtc gtattccgat cgttggcgac
    15121 gaacacgccg acatggaaaa aggcaccggc tgcgtgaaaa tcactccggc gcacgacttt
    15181 aacgactatg aagtgggtaa acgtcacgcg ctgccgatga tcaacatcct gacctttgac
    15241 ggcgatatcc gtgaaagcgc ccaggtgttc gataccaaag gtaacgaatc tgacgtttat
    15301 tccagcgaaa tcccggcaga gttccagaaa ctcgagcgtt ttgctgcacg taaagcagtc
    15361 gttgctgcgg ttgacgcact cggcctgctg gaagaaatta aaccgcacga cctgaccgtt
    15421 ccttacggcg accgtggcgg cgtagttatc gaaccgatgc tgaccgacca gtggtacgtg
    15481 cgtgccgatg tcctggcgaa accggcagtt gaagcggttg agaatggcga cattcagttc
    15541 gtaccgaagc agtacgaaaa catgtacttc tcctggatgc gcgatattca ggactggtgt
    15601 atctctcgtc agctgtggtg gggtcaccga atcccggcat ggtacgacga agcgggtaac
    15661 gtttatgttg gccgcaacga agacgaagtg cgtaaagaaa ataacctcgg tgccgacgtt
    15721 gccctgcgtc aggacgaaga cgttctcgac acctggttct cttctgcgct gtggaccttc
    15781 tctaccctag gctggccgga aaacaccgac gccctgcgtc agttccaccc aaccagcgtg
    15841 atggtatctg gtttcgacat catcttcttc tggattgccc gcatgatcat gatgaccatg
    15901 cacttcatca aagatgaaaa tggcaaaccg caggtgccgt tccacaccgt ttacatgacc
    15961 ggcctgattc gtgatgacga aggccagaag atgtccaaat ccaagggtaa cgttatcgac
    16021 ccgctggata tggttgacgg catttcgctg ccagaactgc tggaaaaacg taccggcaat
    16081 atgatgcagc cgcaactggc ggacaaaatc cgtaagcgca ccgagaagca gttcccgaac
    16141 ggcattgagc cgcacggtac tgatgcgctg cgcttcaccc tggcggcgct ggcttctact
    16201 ggtcgtgaca tcaactggga tatgaagcgt ctggaaggtt accgtaactt ctgtaacaag
    16261 ctgtggaacg ccagccgctt tgtgctgatg aacacagaag gtcaggattg cggcttcaac
    16321 ggcggtgaaa tgacgctgtc gctggcggac cgctggattc tggcagagtt caaccagacc
    16381 atcaaagcgt accgcgaagc gctggacagc ttccgcttcg atatcgccgc aggcattctg
    16441 tatgagttca cctggaacca gttctgtgac tggtatctgg agctgactaa gccggtaatg
    16501 aacggtggca ccgaagccga actgcgcggt actcgccata cgctggtgac cgtgctggaa
    16561 ggtctgctgc gcctcgcgca tccgatcatt ccgttcatca ccgaaaccat ctggcagcgt
    16621 gtgaaagtac tttgcggtat cactgccgac accatcatgc tgcagccgtt cccgcagtac
    16681 gatgcatctc aggttgatga agccgcactg gccgacacag agtggttgaa gcaggcgatc
    16741 gttgcggtac gtaacatccg tgcagaaatg aacatcgcac cgggcaaacc gctggagctg
    16801 ctgctgcgtg gttgcagcgc ggatgcagaa cgtcgcgtaa atgaaaaccg tggcttcctg
    16861 caaaccctgg cgcgtctgga aagcatcacc gtgctgcctg ccgataacaa aggtccggtt
    16921 tccgttacga agatcatcga cggcgcagag ctgctgatcc cgatggctgg cctcatcaac
    16981 aaagaagatg agctggcgcg tctggcgaaa gaagtggcga agatcgaagg cgaaatcagc
    17041 cgcatcgaga ataagctggc gaatgaaggc tttgtcgccc gcgcaccgga agcggtcatc
    17101 gcgaaagagc gtgaaaagct ggaaggctat gcagaagcga aagcgaagct gattgaacag
    17161 caggctgtta tcgccgcgct gtaatcaaat cacagcgatg aaaacgaagg ccgtagcatg
    17221 ctccggcctt ttttatctct tacagaaatg gtaaagaagg cataattccc cgtgaaatcc
    17281 tggctaagaa gcctttatct ggttgttctt ctttattgat taaaggaagg tcatccaaat
    17341 cgcccagcaa atacgtattt tttgcctgcc agtcaatcat ccatattttc agcagtggat
    17401 aagccagacc gcccgtaatc ccggatatca ccaccaacgc acacatgcga taaagcatac
    17461 cgtggtacgt taaagttgag cgaaaacgaa tacgcccatc attcagtgac aggttgctca
    17521 taaaatgatt tcgcaacgac accgttaaat aactggtgct aacagcaatc ccaaaataat
    17581 agattaactg cgcgattatc attttacgtt gcatttccat aaattgctgt aaattctcaa
    17641 tatcatcatt tgcatacaca gaactatcat acgcatttaa tatttgatcg aagataatat
    17701 aaccagcaac agcgagaaaa ggtaataaag ctaaaaatgc gagaatggca tattttatac
    17761 aatacgtagt atctaatttc accttgaaac gatgtatacc gaaactggta ttgctccaga
    17821 gaaaactcat taccaggcta tataaagtac cattaaaaat accaatggaa acaataccaa
    17881 ctattgccat cagaaccaca gatataataa cactacttga actattggca tgtagcatct
    17941 ttgtcgagat aaagaaaaca gctcccatcc caatggccat taaaatcggc aagaaaaagg
    18001 ttacccacca gaaccctttc aaagagcaat taaaactaaa tcttacgccg ttgagactgg
    18061 tcatcaaggc ctgataacgc agtccttttg ctgccataaa tataagcaaa accaacagtg
    18121 acaaagtcaa aacacagcca acaagcggca tatctgctga cactgtcatt aagattgcga
    18181 aatagaaaaa aacaaaaaca agacaactaa caaaaacatt cccaccggta attccataag
    18241 aaaatcgttg tccgttaact tccatattag catagagata acgcttacat ttcattaatg
    18301 cccatggcaa atagatacct agcgttataa tcgttaacaa cacattcacc acacaaataa
    18361 gaaaatatct ctcacctgtg ccatgaaaaa caaacgaatg ggacggaaca tccatttcat
    18421 taataacttg agccataaaa attccctgaa tt
//
LOCUS       DAJOWB010000090        18437 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJOWB010000090 DAJOWB010000000
VERSION     DAJOWB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.090"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            340..555
                     /gene="essD"
                     /locus_tag="OHO85_003366"
     CDS             340..555
                     /gene="essD"
                     /locus_tag="OHO85_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCQ2672702.1"
                     /translation="MKFMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            555..1052
                     /gene="rrrD"
                     /locus_tag="OHO85_003367"
     CDS             555..1052
                     /gene="rrrD"
                     /locus_tag="OHO85_003367"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HCQ2672703.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            1269..1451
                     /gene="rzoD"
                     /locus_tag="OHO85_003368"
     CDS             1269..1451
                     /gene="rzoD"
                     /locus_tag="OHO85_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HCQ2672704.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPRPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1542..1835)
                     /gene="bor"
                     /locus_tag="OHO85_003369"
     CDS             complement(1542..1835)
                     /gene="bor"
                     /locus_tag="OHO85_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HCQ2672705.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKQTAVAPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSK"
     gene            complement(2126..2536)
                     /locus_tag="OHO85_003370"
     CDS             complement(2126..2536)
                     /locus_tag="OHO85_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCQ2672706.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSELKRHNVSHYIYYLATDNI
                     HIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSYDRLKSREITFQQYRENLAKAGVFR
                     WITNIHEHKRYYYTFDNSLLFTESIQNTTQIFPR"
     gene            2822..3028
                     /locus_tag="OHO85_003371"
     CDS             2822..3028
                     /locus_tag="OHO85_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672707.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            complement(3193..3387)
                     /locus_tag="OHO85_003372"
     CDS             complement(3193..3387)
                     /locus_tag="OHO85_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCQ2672708.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIKSDEE"
     gene            3776..4321
                     /locus_tag="OHO85_003373"
     CDS             3776..4321
                     /locus_tag="OHO85_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCQ2672709.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            4296..6221
                     /locus_tag="OHO85_003374"
     CDS             4296..6221
                     /locus_tag="OHO85_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCQ2672710.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            6218..6424
                     /locus_tag="OHO85_003375"
     CDS             6218..6424
                     /locus_tag="OHO85_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HCQ2672711.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            6421..8022
                     /locus_tag="OHO85_003376"
     CDS             6421..8022
                     /locus_tag="OHO85_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCQ2672712.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            8003..9322
                     /locus_tag="OHO85_003377"
     CDS             8003..9322
                     /locus_tag="OHO85_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCQ2672713.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            9332..9664
                     /locus_tag="OHO85_003378"
     CDS             9332..9664
                     /locus_tag="OHO85_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCQ2672714.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGTAVGILAVDADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            9720..10745
                     /locus_tag="OHO85_003379"
     CDS             9720..10745
                     /locus_tag="OHO85_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCQ2672715.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSNSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVENDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            10787..11182
                     /locus_tag="OHO85_003380"
     CDS             10787..11182
                     /locus_tag="OHO85_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCQ2672716.1"
                     /translation="MTKDELIARLQVLGEQLNRDVSLTGTKEELVLRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQPDTVIDTAALVTVVALVTLHTDALHATRDEA
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            11194..11547
                     /locus_tag="OHO85_003381"
     CDS             11194..11547
                     /locus_tag="OHO85_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCQ2672717.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            11559..12137
                     /locus_tag="OHO85_003382"
     CDS             11559..12137
                     /locus_tag="OHO85_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2672718.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSVAQV
                     ARETKVRRKLVKERARLKRATVKNPQARIRVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            12134..12528
                     /locus_tag="OHO85_003383"
                     /pseudo
     CDS             12134..12528
                     /locus_tag="OHO85_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            12536..13276
                     /locus_tag="OHO85_003384"
     CDS             12536..13276
                     /locus_tag="OHO85_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2672719.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKDVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTAS"
     gene            13292..13714
                     /locus_tag="OHO85_003385"
     CDS             13292..13714
                     /locus_tag="OHO85_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCQ2672720.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPKKTKLPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQTEDAGPAEPVSAGKCSMVS"
     gene            13696..14130
                     /locus_tag="OHO85_003386"
     CDS             13696..14130
                     /locus_tag="OHO85_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCQ2672721.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            14123..16702
                     /locus_tag="OHO85_003387"
     CDS             14123..16702
                     /locus_tag="OHO85_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCQ2672722.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTETDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLTALVNAGVRGGEQFEAISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDSVLDIGRPDTAQGMLEKAEKAFDE
                     ADKKWQWYQSRSHRRGKTSAFLANLRGAWEDRANAQLGLSAATLQADLEKAREMAAKD
                     WAESEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAK
                     KDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDL
                     WKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQ
                     QADKFAQQQRAKWAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKT
                     WAAEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFA
                     TGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQ
                     MRRAEGINFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQAQMRDGGLFSG
                     GGR"
     gene            16699..17028
                     /locus_tag="OHO85_003388"
     CDS             16699..17028
                     /locus_tag="OHO85_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2672723.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            17028..17726
                     /locus_tag="OHO85_003389"
     CDS             17028..17726
                     /locus_tag="OHO85_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCQ2672724.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSGADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            17732..>18437
                     /locus_tag="OHO85_003390"
     CDS             17732..>18437
                     /locus_tag="OHO85_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2672725.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTG"
BASE COUNT         4353 a         4485 c         5506 g         4093 t
ORIGIN      
        1 atttaaacca gctaaaaata atactggaaa ttatttgttg gttatttgtt gaagttttct
       61 tatgtatttg tggtgatgtt ttgaacactc ggtagcattc tcataaatat cattcagtgg
      121 tttacgtacg taaaaaattg gttatgctgt taagagtggt tacttcgtca cacagcttaa
      181 acccgccgtc gagctggttt ttccattttt tgagtctcga tattagctga taactcaata
      241 cctgagttat tcactgactc ggagtcagtt acgtttctgc ttttttgcga tacgttgtat
      301 tccctcaatt tacacccgct ttgtctgcga ggtggggtta tgaaattcat ggataagtta
      361 acaactggag tcgcctatgg cacctcagca ggtagtgccg gttactggtt tttacagctg
      421 ctcgataaag tcactccctc acagtgggca gcaataggtg tgctgggtag cctggtattt
      481 ggcctgctga cgtacctgac aaacctttat ttcaagatta aagaagataa gcgcaaggct
      541 gcgagaggtg aataatgcct ccatcattac gaaaagccgt tgctgctgct attggtggcg
      601 gagcaattgc tatagcatca gtgttaatca ctggcccaag tggtaacgat ggtctggaag
      661 gtgtcagcta cataccatac aaagatattg ttggtgtatg gactgtatgt cacggacaca
      721 ccggaaaaga catcatgctc ggtaaaacgt ataccaaagc agaatgcaaa gcactcttga
      781 ataaagacct tgccactgtc gccagacaaa ttaacccgta tatcaaagtc gatataccgg
      841 aaacaacgcg cggcgctctt tactcattcg tttacaacgt gggtgctggc aattttagaa
      901 catcgacgct tcttcgcaaa ataaaccagg gcgatatcaa aggcgcatgt gatcagctgc
      961 gtcgctggac atacgctggc ggtaagcaat ggaaaggcct gatgactcgt cgtgagattg
     1021 agcgtgaagt ctgtttgtgg gggcaacagt gagcagagta accgcgatta tatccgctct
     1081 gattatctgc atcatcgtca gcctgtcatg ggcggtcaat cattaccgtg ataacgcaat
     1141 cgcctacaaa gtccagcgcg acaaaaatgc cagagaactg aagctagcga acgcggcaat
     1201 tactgacatg cagatgcgtc agcgtgatgt tgctgcgctc gatgcaaaat acacgaagga
     1261 gttagctgat gcgaaagctg aaaatgatgc tctgcgtgat gatgttgccg ctggtcgtcg
     1321 tcggttgcac atcaaagcag tctgtcagtc agtgcgtgaa gccaccacgg cctccggcgt
     1381 ggataatgca gcctcccccc gactggcaga caccgctgaa cgggattatt tcaccctcag
     1441 agagaggctg atcactatgc aaaaacaact ggaaggaacc cagaagtata ttaatgagca
     1501 gtgcagatag agctgaccat atcgatgggc aactcatgca attattttga gcaatacaca
     1561 cgcgcttcca gcggagtata aatgcctaaa gtaataaaac cgagcaatcc atttacgaat
     1621 gtttgctggg tttctgtttt aacaacattt tctgcgccgc cacaaatttt agctgcatcg
     1681 acagttttct tctgcccaat tccagaaacg aagaaatgat gggtgatggt ttcctttggt
     1741 gctactgctg tctgtttgtt ttgaacagta aatgtctgtt gagcacatcc tgtaataagc
     1801 agggccagcg cagtagcgag tagcattttt ttcatggtgt tattcccgat gctttttgaa
     1861 gttcgcagaa tcgtatgtgt agaaaattaa acaaacccta aacaatgagt tgaaatttca
     1921 tattgttaat atttattaat gtatgccagg tgcgatgaat cgtcattgta ttcccggatt
     1981 aactatgtcc acagccctga cggggaactt ctctgcggga gtgtccggga ataattaaaa
     2041 acgatgcaca cagggtttag cgcgtacatg tattgtatta tgccaacacc ccggtgctga
     2101 cacggaagaa accggacgtt atgatttagc gtggaaagat ttgtgtagtg ttctgaatgc
     2161 tctcagtaaa tagtaatgaa ttatcaaagg tatagtaata tcttttatgt tcgtggatat
     2221 ttgtaatcca tcggaaaact cctgctttag caagattttc cctgtattgc tgaaatgtga
     2281 tttctcttga tttcaaccta tcataggacg tttctataag atgcgtattt cttgagaatt
     2341 taacatttac aaccttttta agccctttta ttaacacggt gttatcgttt tctaacacaa
     2401 tgtgaatatt atctgtggct agatagtaaa tataatgtga gacattgtga cgttttagtt
     2461 cagaataaaa caattcacag tttaaatctt ttcgcacttg atcgaatatt tctttaaaaa
     2521 tggcaacctg agccattggt aaaaccttcc atgtgatacg agggcgcgta gtttgcatta
     2581 tcgtttttat cgcttcaatc tggtctgacc tctttgtgtt ttgttgatga tttatgtcaa
     2641 atattaggaa tgttttcaat taatagtatt ggttgcgtaa caaagtgcgg tcctgctggc
     2701 attctggagg gaaatacaac cgacagatgt atgtaaggcc aacgtgctca aaccttcata
     2761 cagaaagatt tgaagtaata ttttaaccgc tagatgaaga gcaagcgcat ggagcgacaa
     2821 aatgaataaa gaacaatctg ctgatgatcc ctccgtggat ctgattcgtg taaaaaatat
     2881 gcttaatagc accatttcta tgagttaccc tgatgttgta attgcatgta tagaacataa
     2941 ggtgtctctg gaagcattca gggcaattga ggcagcgttg gtgaagcacg ataataatat
     3001 gaaggattat tccctggtgg ttgactgatc accataactg ctaatcattc aaactactta
     3061 acctgtgaca gagccaacac gcagtctgtc actgtcagga aagtggtaaa actgcaactc
     3121 aattactgca atgccctcgt aattaagtga atttacaata tcgtcctgtt cggagggaag
     3181 aacgcgggat gttcattctt catcactttt aattgatgta tatgctctct tttctgacgt
     3241 tagcctccga cggcaggctt caatgaccca ggctgagaaa ttcccggacc ctttttgctc
     3301 aagagcgatg ttaatttgtt caatcatttg gttaggaaag cggatgttgc gggttgttgt
     3361 tctgcgggtt ctgttcttag ttgacatgag gttgccccgt attcagtgtc gctgatttgt
     3421 attgtctgaa gttgttttta cgttaagttg atgcagatca attaatacga tacctgcgtc
     3481 ataattgatt atttgacgtg gtttgatggc gtagatgcac gttgtgatat gtagatgata
     3541 attattatca ttttgtgggt cctttccggc gatccgacag gttacggggc ggcgacctcg
     3601 cgggttttcg ctatttatga aaattttccg gtttaaggca tttccgttct tcttcgtcgt
     3661 aacttaatgt ttttatttaa aataccccct gaaaagaaag gaaacgacag gtgctgaaaa
     3721 cgagcttttg ggcctctgtc gtttcctttc tctgtttttg gccgtggaat gaacaatgga
     3781 agtcaacaaa aagcagctgg ctgacatttt cggtgcgagt atccgtacca ttcagaactg
     3841 gcaggaacag ggaatgcccg ttctgcgagg cggtggcaag ggtaatgagg tgctttatga
     3901 ctctgccgcc gttataagat ggtatgccga aagggatgct gaaattgaga acgaaaagct
     3961 gcgccgggaa gttgaagaac tgcggcaggc cagcgagaca gatctccagc cagggactat
     4021 tgagtacgaa cgccatcgac ttacgcgtgc gcaggccgac gcacaggagc tgaaaaatgc
     4081 cagagactcc gctgaagtgg tggaaaccgc attctgtact ttcgtgctgt cgcggatcgc
     4141 aggtgaaatt gccagtattc tcgacgggat ccccctgtcg gtgcagcggc gttttccgga
     4201 actggaaaac cgacatgttg atttcctgaa acgggatatc atcaaagcca tgaacaaagc
     4261 agccgcgctg gatgaactga taccggggtt gctgagtgaa tatatcgaac agtcaggtta
     4321 acaggctgcg gcattttgtc cgcgccgggc ttcgctcact gttcaggccg gagccacaga
     4381 ccgccgttga atgggcggat gctaattact atctcccgaa agaatccgca taccaggaag
     4441 ggcgctggga aacactgccc tttcagcggg ccatcatgaa tgcgatgggc agcgactaca
     4501 tccgtgaggt gaatgtggtg aagtctgccc gtgtcggtta ttccaaaatg ctgctgggtg
     4561 tttatgccta ctttatagag cataagcagc gcaacacact tatctggttg ccgacggatg
     4621 gtgatgccga gaactttatg aaaacccacg ttgagccgac catccgcgat attccgtcgc
     4681 tgctggcgct ggctccgtgg tatggcaaaa agcaccggga taacacgctc actatgaagc
     4741 gtttttccaa tggtcgtggc ttctggtgcc tgggcggtaa agcggcaaaa aactaccgtg
     4801 aaaagtcggt ggatgtggcg ggttatgatg aacttgctgc ctttgatgag gatattgaac
     4861 aggaaggctc tccgacgttc cttggcgaca aacgtattga aggctcggtc tggccaaagt
     4921 ccatccgtgg ctccacgccc aaagtgagag gcacctgcca gattgagcgt gcagccagtg
     4981 aatccccgca ttttatgcgt tttcatgttg cctgcccgca ctgcggggag gagcagtatc
     5041 ttaaatttgg cgacaaagag acgccgtttg gcctcaaatg gacgccggat gacccctcca
     5101 gcgtgtttta tctctgcgag cataatgcct gcgtcatccg ccagcaggag ctggacttta
     5161 ctgatgcccg ttatatctgc gaaaagaccg ggatctggac ccgtgatggc attctctggt
     5221 tttcgtcatc cggtgaagag attgagccac ctgacagtgt gacctttcac atctggacag
     5281 cgtacagccc gttcaccacc tgggtgcaga ttgtcaaaga ctggatgaaa acgaaagggg
     5341 atacgggaaa acgtaaaacc ttcgtaaaca ccacgctcgg tgagacgtgg gaggcgaaaa
     5401 ttggcgaacg tccggatgct gaagtgatgg cagagcggaa agagcattat tcagcgcccg
     5461 ttcctgaccg tgtggcttac ctgaccgccg gtatcgactc ccagctggat cgctacgaaa
     5521 tgcgcgtatg gggatggggg ccgggtgagg aaagctggct gattgatcgg cagattatta
     5581 tgggccgcca cgacgatgaa cagacgctgc tgcgtgtgga tgaggccatc aataaaacct
     5641 atacccgccg gaatggtgca gaaatgtcga tatcccgtat ctgctgggat attggcggga
     5701 ttgacccgac cattgtgtat gaacgctcga aaaagcatgg gctgttccgg gtgatcccca
     5761 ttaaaggggc atccgtctac ggtaagccgg tggccagcat gcctcgtaag cgaaacaaaa
     5821 acggggttta ccttaccgaa atcggtacgg ataccgcgaa agagcagatt tataaccgct
     5881 tcacactgac gccggaaggg gatgaaccgc ttcccggtgc cgttcacttc ccgaataacc
     5941 cggatatttt tgatctgacc gaagcgcagc agctgactgc tgaagagcag gtcgaaaaat
     6001 gggtggatgg caggaaaaaa atactgtggg acagcaaaaa gcgacgcaat gaggcgctcg
     6061 actgcttcgt ttatgcgctg gcggcgctgc gcatcagtat ttcccgctgg cagctggatc
     6121 tcagtgcact gctggcgagc ctgcaggaag aggatggtgc agcaaccaac aagaaaacac
     6181 tggcagatta cgcccgtgcc ttatccggag aggatgaatg acgcgacagg aagaacttgc
     6241 cgctgcccgt gcggcactgc atgacctgat gacaggtaaa cgggtggcaa cggtacagaa
     6301 agacggacgg cgagtggagt ttacggccac ttccgtgtct gacctgaaaa aatacattgc
     6361 ggagctggag gtgcagaccg gcatgacaca gcgacgcagg ggacctgcag gattttatgt
     6421 atgaaaacgc ccaccattcc cacccttctg ggaccggacg gcatgacatc gctgcgtgaa
     6481 tatgccggtt atcacggcgg tggcagcgga tttggtgggc agttgcgggc gtggaaccca
     6541 ccgggtgaaa gtgtggatgc agccctgctg cccaacttta cccgtggcaa tgcccgcgca
     6601 gacgatctgg tacgcaataa cggctatgct gccaacgcca tccagttgca tcaggatcat
     6661 atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct gggcatcggg
     6721 gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga gtttgccgag
     6781 gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat gattcgggaa
     6841 ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg ggataccagc
     6901 ccgtcgcgac tgttccggac acagttccgg atggtcagcc cgaagcgcat cagcaacccg
     6961 aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga cagcggtgcg
     7021 gcgctgggat attacgtcag cgaggacggg tatcctggct ggatgccgca gaaatggaca
     7081 tggatacccc gtgagttacc cggcgggcgc gcctcgttca ttcacgtttt tgaacccgtg
     7141 gaggacgggc agacccgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
     7201 ctcgacacgc tgcagaacac gcagctgcag agcgccattg tgaaggcgat gtatgccgcc
     7261 accattgaaa gtgagctgga tacgcagtca gcgatggatt ttattctggg cgcgaacagt
     7321 caggagcagc gggaaaggct gacgggctgg attggtgaaa ttgccgcgta ttacgccgca
     7381 gcaccggtcc gtctgggagg cgcaaaagtg ccgcacctga tgccggggga ctcactgaac
     7441 ctgcagacgg ctcaggatac ggataacggc tactccgtgt ttgagcagtc actgttgcgg
     7501 tatatcgctg ccgggctggg tgtctcgtat gagcagcttt cccggaatta cgcccagatg
     7561 agctactcca cggcacgggc cagtgcgaac gagtcgtggg cgtactttat ggggcggcga
     7621 aaattcgtcg catcccgtca ggcgagccag atgtttctgt gctggcttga agaggccatc
     7681 gttcgccgcg tggtgacgtt accttcaaaa gcgcgcttca gctttcagga agcccgcagt
     7741 gcctggggga actgcgactg gataggctcc ggtcgtatgg ccatcgatgg tctgaaagaa
     7801 gttcaggaag cggtgatgct gatagaagcc ggactgagca cctacgagaa agagtgcgcg
     7861 aaacgcggtg acgactatca ggaaattttt gcccagcagg tccgtgaaac gatggagcgc
     7921 cgtgcagccg gtcttaaacc gcccgcctgg gcggctgcgg catttgaatc cggactgcga
     7981 caatcaacag aggaggagaa gagtgacagc agagctgcgt aatctcccgc atattgccag
     8041 catggctttt aatgagccgc tgatgcttga acccgcctat gcgcgggttt tcttttgtgc
     8101 gcttgcaggc cagcttggga tcagtcgcct gacggatgca gtatccggcg acagcctgac
     8161 tgccggagag gcacccgcgg cgctggcgtt atccggtgat gatgacggac cacgacaggc
     8221 ccgcagttat caggtcatga acggcatcgc cgtgctgccg gtgtccggta cgctggtcag
     8281 ccggacgcgg gcgctgcagc cgtattcggg aatgaccggt tacaacggca ttatcgctcg
     8341 tctgcaacag gctgccagcg atccgatggt ggacggcatt ctgctcgata tggacacacc
     8401 gggcgggatg gtggcgggag catttgactg tgctgacatc atcgcccgtg tgcgtgacat
     8461 aaagccggta tgggcgctgg ccaacgacat gaactgcagt gcaggtcagc tgcttgccag
     8521 cgccgcctcc cggcgtctgg tcacgcagac cgcccggaca ggctccatcg gcgtcatgat
     8581 ggctcacagt aattacggtg ctgcgctgga gaaacagggc gtggaaatca cgctgattta
     8641 cagcggcagc cataaggtgg atggcaaccc ctacagccat ctaccggatg atgtccggga
     8701 aacactgcag tcccggatgg atgcaacccg ccggatgttt gcacagaagg tgtcggcata
     8761 taccggcctg tccgtgcagg ctgtgctgga taccgaggct gcagtgtaca gcggtcagga
     8821 ggccattgat gccggactgg ctgatgaact tgtcaacagc accgatgcga tcaccgttat
     8881 gcgtgatgca ctggatgcac gtaaatcccg tctctcagga gggcgaatga ccaaagagac
     8941 tcaatcaaca actgtttcag ccactgcttc gcaggctgac gttactgacg tggtgcaagc
     9001 gacggagggc gaaaacgcca gcgcggcgca gccggacgtg aacgcgcaga tcaccgctgc
     9061 ggttgcggca gaaaacagcc gcattatggg gatcctcaac tgtgaggaag ctcacggacg
     9121 cgaagaacag gcacgtgtgc tggccgaaac ccccggtatg accgtggaaa cggcccgccg
     9181 cattctggca gctgcaccac agagtgcaca ggcgcgcagt gacactgcgc tggatcgtct
     9241 gatgcagggg gcaccggcac cactggctgc aggtaacccg gcatctgatg ccgttaacga
     9301 tttgctgaac acaccagtgt aagggatgtt tatgacgagc aaagaaacct ttacccatta
     9361 ccagccgctg ggcaacagtg acccggcaca tacggcaacc gcgcccggcg gattgagtgc
     9421 gaaagcgcct gcaatgaccc cgctgatgct ggacacctcc acccgtaagc tggttgcgtg
     9481 ggatggcacc accgacggta ctgccgttgg cattctggcg gttgatgctg accagaccag
     9541 caccacgctg acgttctaca agtccggcac gttccgttat gaggatgtgc tctggccgga
     9601 ggctgccagc gacgagacga aaaaacggac cgcgtttgcc ggaacggcaa tcagcatcgt
     9661 ttaaccttac ccttcatcac taaaggccgc ctgtgcggct ttttttacgg gattttttta
     9721 tgtcgatgta cacaaccgcc cagctgctgg cggcaaatga gcagaaattt aagtttgatc
     9781 cgctgtttct gcgtctcttt ttccgtgaga gctatccctt caccacggag aaagtctatc
     9841 tctcacaaat tccgggactg gtaaacatgg cgctgtacgt ttcgccgatt gtttccggtg
     9901 aggttatccg ttcccgtggc ggctccacct ctgaatttac gccgggatat gtcaaaccca
     9961 agcatgaggt gaatccgcag atgaccctgc gtcgcctgcc ggatgaagat ccgcagaatc
    10021 tggcggaccc ggcttaccgc cgccgtcgca tcatcatgca gaacatgcgt gacgaagagc
    10081 tggccattgc tcaggtcgaa gagatgcagg cagtttctgc tgtgcttaag ggcaaataca
    10141 ccatgaccgg tgaagccttc gatccggttg aggtggatat gggccgcagt gcggcgaaca
    10201 acatcacaca gtccggcgtc acggagtgga gcaagcgtga caagtccacg tatgacccga
    10261 ccgacgatat cgaagcctac gcgctgaacg ccagcggtgt ggtgaatatc atcgtgtttg
    10321 acccgaaagg ctgggcgctg ttccgttcct tcaaagccgt cagggagaag ctggataccc
    10381 gtcgcggctc taattccgag ctggagacag cggtaaaaga cctgggcgaa gcggtgtcct
    10441 ataaggggat gtatggcgat acggcgatcg tcgtgtattc cggacagtac gtggaaaacg
    10501 acgtcaaaaa gaacttcctg ccggacaaca cgatggtgct ggggaacact caggcacgcg
    10561 gtctgcgcac ctatggctgc attcaggatg cggacgcaca gcgcgaaggt attaacgcct
    10621 ctgcccgcta cccgaaaaac tgggtgacca ccggcgatcc ggcgcgtgag ttcaccatga
    10681 ttcagtcagc accgctgatg ctgctggctg atcctgatgc gttcgtgtcc gtacaactgg
    10741 cgtaatcatg gcccttcggg gccattttct ctctgtggag gagtccatga cgaaagatga
    10801 actgattgcc cgtcttcagg tgctgggtga gcaactgaac cgtgatgtca gcctgacggg
    10861 gacgaaagaa gaactggtgc tccgtgtggc agagctggaa gaggagcttg atgacacgga
    10921 tgacgctgcc ggtcaggaca catctgtcag cccggaaaat gcgctgaccg gacatgaaaa
    10981 tgaggtggta tcagcgcagc cggataccgt gattgatacg gctgctctgg tcacggtcgt
    11041 ggcactggtg acgctgcata ccgatgcact tcacgccacg cgggatgagg ctgtggcatt
    11101 tgtgctgccg ggaacggcgt tccgtgtctc tgccggtgtg gcagctgaaa tgacagagcg
    11161 cggcctggcc agaatgcaat aacgggaggc gctgtggctg atttcgataa cctgttcgat
    11221 gctgccattg cccgcgccga tgaaacgata cgcgggtaca tgggaacgtc agccaccatg
    11281 acatccggtg agcagtccgg cgcagtaata cgtggtgttt ttgatgaccc tgaaaatatc
    11341 agctatgccg gacagggcgt gcgcgttgaa ggctccagcc cgtccctgtt tgtccggact
    11401 gatgatgtgc ggcaactgcg gcgtggagac acgctgacca tcggtgagga aaacttctgg
    11461 atagaccggg tttcgccgga tgatggtgga agctgtcatc tctggcttgg gcggggcgta
    11521 ccgcctgccg ttaaccgtcg ccgctgaaag ggggatgtat ggccataaaa ggtcttgagc
    11581 aggccgttga aaacctcagc cgtatcagca aaacggcggt gcctggtgcc gccgcaatgg
    11641 ccattaaccg cgttgcgtca tccgcgatat cgcagtcggt ggcacaggtt gcccgtgaga
    11701 caaaggtacg ccggaaactg gtaaaggaaa gggccaggct gaaaagggcc acggtcaaaa
    11761 atccgcaggc cagaatcagg gttaaccggg gggatttgcc cgtaatcaag ctgggtaacg
    11821 cgcgggttgt cctttcccgc cgcagacgtc gtaaaaaggg gcagcgttca tccctgaaag
    11881 gtggcggcag cgtgcttgtg gtgggaaacc gtcgtattcc cggcgcgttt attcagcaac
    11941 tgaaaaatgg ccggtggcat gtcatgcagc gtgtggccgg gaaaaaccgt taccccattg
    12001 atgtggtgaa aatcccgatg gcggtgccgc ttaccacggc gtttaaacag aatattgaac
    12061 ggatacggcg tgaacgtctt ccgaaagagc tgggctatgc gctgcagcat caactgagaa
    12121 tggtaataaa gcgatgaaac atactgaact ccgtgcagcc gtactggatg cactggagaa
    12181 gcatgacacc ggggcgacgc tttttgatgg tcgccccgct gtttttgatg aggcggattt
    12241 tccggcaatt gccgtttatc tcaccggcgc tgaatacacg ggcgaagagc tggacagcga
    12301 tacctggcag gcggagctgc atatcgaagt tttcctgcct gctcaggtgc cggattcaga
    12361 gctggatgcg tggatggagt cccggattta tccggtgatg agcgatatcc cggcactgtc
    12421 agatttgatc accagtatgg tggccagcgg ctatgactac cggcgcgacg atgatgcggg
    12481 cctgtgagtt cagccgatct gacttatgtc attacctatg aaatgtgagg acgctatgcc
    12541 tgtaccaaat cctacaatgc cggtgaaagg tgccgggacc accctgtggg tttataaggg
    12601 gagcggtgac ccttatgcga atccgctttc agacgttgac tggtcgcgtc tggcaaaagt
    12661 taaagacctg acgcccggcg aactgaccgc tgagtcctat gacgacagct atctcgatga
    12721 tgaagatgca gactggactg cgacagggca ggggcagaaa tctgccggag ataccagctt
    12781 cacgctggcg tggatgcccg gagagcaggg gcagcaggcg ctgctggcgt ggtttaatga
    12841 aggcgatacc cgtgcctata aaatccgctt cccgaacggc acggtcgatg tgttccgtgg
    12901 ctgggtcagc agtatcggta aggctgtgac ggcgaaggac gtgatcaccc gcacggtgaa
    12961 agtcaccaat gtgggacgtc cgtcgatggc agaagatcgc agcacggtaa cagcggcaac
    13021 cggcatgacc gtgacgcctg ccagcacctc ggtggtgaaa gggcagagca ccacgctgac
    13081 cgtggccttc cagccggagg gcgtaaccga caagagcttc cgtgcggtgt ctgcggataa
    13141 aacaaaagcc accgtgtcgg tcagtggtat gaccatcacc gtgaaaggtg ttgctgcagg
    13201 caaggtcaac attccggtcg tatccggtaa tggtgaactt gctgcggttg cagaaatcac
    13261 cgtcaccgcc agttaatccg gggagtcaga gatgttcctg aaaaccgaat catttgaaca
    13321 taacggtgtg accgtcacgc tttctgaact gtcagccctg cagcgtattg agcatctcgc
    13381 cctgatgaaa cggcaggcag aacaggcgga gtcagacagc aaccggaagt ttactgtgga
    13441 agacgccatc agaaccggcg cgtttctggt ggcgatgtcc ctgtggcata accatccgaa
    13501 gaagacaaag ctgccttcca tgaatgaagc cgttaaacag attgagcagg aagtgcttac
    13561 cacctggccc acggaggcaa tttctcatgc tgaaaacgtg gtgtaccggc tgtccggtat
    13621 gtatgagttt gtggtgaatg atgctcctga acagacagag gacgccgggc ctgcagagcc
    13681 tgtttctgcg ggaaagtgtt cgatggtgag ctgagttttg ccctgaaact ggcgcgagag
    13741 atggggcgac ccgactggcg tgccatgctt gccgggatgt catccacgga gtatgccgac
    13801 tggcaccgct tttacagtac ccattatttt catgatgttc tgctggatat gcacttttcc
    13861 gggctgacgt acaccgtgct cagcctgttt ttcagcgatc cggatatgca tccgctggat
    13921 ttcagtctgc tgaaccggcg tgaggctgac gaagagcctg aagatgatgt gctgatgcag
    13981 aaagcggcag ggcttgccgg aggcgtccgc tttggcccgg acgggaatga agttatcccc
    14041 gcttccccgg atgtggcgga catgacggag gatgacgtaa tgctgatgac agtatcagaa
    14101 gggatcgcag gaggagtccg gtatggctga accggtaggc gatctggtcg ttgatttaag
    14161 tctggatgcg gccagatttg acgagcagat ggccagagtc aggcgtcatt tttccggtac
    14221 ggaaactgat gcgaaaaaaa cagcggcagt cgttgaacag tcgctgagcc gacaggcgct
    14281 ggctgcacag aaagcgggga tttccgtcgg gcagtataaa gccgccatgc gtatgctgcc
    14341 tgcacagttc accgacgtgg ccacgcagct tgcaggcggg caaagcccgt ggctgatcct
    14401 gctgcaacag gggggtcagg ttaaggactc cttcggcggg atgatcccca tgttcagggg
    14461 gcttgccggt gcgatcaccc tgccgatggt gggggccacc tcgctggcgg tggcgaccgg
    14521 tgcgctggcg tatgcctggt atcagggcaa ctcaaccctg tccgatttca acaaaacgct
    14581 ggtcctttcc ggcaatcagg cgggactgac ggcagatcgt atgctggtcc tgtccagagc
    14641 cgggcaggcg gcagggctga cgtttaacca gaccagcgag tcactgacgg cgctggtgaa
    14701 tgccggtgtg cgtggtggtg agcagtttga ggcgatcagc cagagtgtgg cgcgtttctc
    14761 ctctgcatcc ggcgtggagg tggacaaggt cgctgaagcc ttcgggaagc tgaccacaga
    14821 cccgacgtcg ggactgacag cgatggcacg tcagttccat aacgtgacgg cggagcagat
    14881 tgcgtatgtt gctcagttgc agcgttccgg agatgaagcc ggggcattgc aggcggcgaa
    14941 cgaggccgca acgaaagggt ttgatgacca gacccgccgc ctgaaagaga acatgggcac
    15001 gctggagacc tgggcagaca ggacagcacg ggcattcaaa tccatgtggg attcggtgct
    15061 ggatattggt cgcccggaca ctgcccaggg aatgctggag aaagcagaaa aggcttttga
    15121 tgaggcggac aaaaaatggc agtggtatca gagccggagc caccggcgcg gtaaaacctc
    15181 agcatttctt gccaatctcc ggggagcatg ggaggacaga gcgaatgcgc aacttgggct
    15241 ttcagccgcc acgttgcagg ccgatcttga aaaggccaga gagatggcag caaaggactg
    15301 ggccgagtct gaggcatcac ggctgaaata taccgaagag gcgcagaagg cttacgaacg
    15361 gctgcagacg ccgctggaga aatataccgc ccgtcaggaa gaactgaaca aggcactgaa
    15421 agacgggaaa atcctgcagg cggattacaa cacgctgatg gcggcggcga aaaaggatta
    15481 tgaagcgacg ctgaaaaagc cgaaacagtc cggcgtgaag gtgtctgcgg gcgatcgtca
    15541 ggaagacagt gctcatgctg ccctgctgac gcttcaggct gaactccgga cgctggagaa
    15601 gcatgccgga gcaaatgaga aaatcagcca gcagcgccgg gatttgtgga aggcggagag
    15661 ccagttcgcg gtactggagg aggcggcgca acgtcgccag ctgtctgcac aggagaaatc
    15721 cctgctggcg cataaagatg agacgctgga gtacaaacgc cagctggctg cacttggcga
    15781 caaggttacg tatcaggagc gcctgaacgc gctggcgcag caggcggata aattcgcaca
    15841 gcagcaacgg gcaaaatggg ccgccattga tgcgaaaagc cgggggctga ctgaccggca
    15901 ggcagaacgg gaagccacgg aacagcgcct gaaggaacag tatggcgata atccgctggc
    15961 gctgaataac gtcatgtcag agcagaaaaa gacctgggcg gctgaagacc agcttcgcgg
    16021 gagctggatg gcaggcctga agtccggctg gagtgagtgg gaagagagcg ccacggacag
    16081 tatgtcgcag gtaaaaagtg cagccacgca gacctttgat ggtattgcac agaatatggc
    16141 ggcgatgctg accggcagtg agcagaactg gcgcagcttc acccgttccg tgctgtccat
    16201 gatgacagaa attctgctta agcaggcaat ggtggggatt gtcgggagta tcggtagcgc
    16261 cattggcggg gctgttggtg gcggcgcatc cgcatcaggc ggtacagcca ttcaggccgc
    16321 tgcggcgaaa ttccattttg caaccggagg atttacggga accggcggca aatatgagcc
    16381 agcggggatt gttcaccgtg gtgaatttgt cttcacgaag gaggcaacca gccgaattgg
    16441 tgtcggcaac ctgtaccgcc tgatgcgggg ctatgcggaa ggtggttatg tgggcggtgc
    16501 cggaagtccg gcgcagatgc ggcgggctga aggcattaat tttaatcaga acaatcacgt
    16561 ggtgattcag aacgacggta cgaatggtct gccaggtcca cagatgatga aggcagtgta
    16621 tgacatggcc cgcaagggtg cccgtgatga aattcaggca cagatgcgcg atggtggtct
    16681 gttctccgga ggtggacgat gaagaccttc cgctggaaag tgaaacccgg tatggatgtg
    16741 gcttcggccc cttccgtcag gaaagtgcgc tttggtgatg gctattccca gcgagcgcct
    16801 gccgggctga atgccgacct gaaaacgtac agcgtgacgc tttctgttcc ccgttgggag
    16861 gccacggcgc tggagtcgtt tctggctgag cacgggggct ggaaagcctt tctgtggacg
    16921 ccgccttatg agtggcggca gataaaggtg acctgcgcaa aatggtcgtc gcgggtcagt
    16981 atgctgcgtg ttgagttcag cgcagagttt gaacaggtgg tgaactgatg caggatatcc
    17041 ggcaggaaac actgaatgaa tgcacccgtg cggagcagtc ggccagcgtg gtgctctggg
    17101 aaatcgacct gacagaggtc ggtggagaac gttatttttt ctgtaatgag cagaacgaaa
    17161 aaggtgagcc ggtcacctgg caggggcgac agtatcagcc gtatcccatt caggggagtg
    17221 gttttgaact gaatggcaaa ggcaccagta cgcgccccac actgacggtt tctaacctgt
    17281 acggtatggt caccgggatg gcggaagatc tgcagagtct ggtcggtgga acggtggtcc
    17341 ggcgtaaggt ttacgcccgt tttctggatg cggtgaactt cgtcaacgga aacagtggcg
    17401 ccgatccgga gcaggaggtg atcagccgct ggcgcattga gcagtgcagc gaactgagcg
    17461 cggtgagtgc ctcttttgta ctgtccacgc cgacggaaac ggatggcgct gtttttccgg
    17521 gacgtatcat gctggccaac acctgcacct ggacctatcg cggtgacgag tgcggttata
    17581 gcggtccggc tgtcgcggat gaatatgacc agccaacgtc cgatatcacg aaggataaat
    17641 gcagcaaatg cctgagcggt tgtaagttcc gcaataacgt cggcaacttt ggcggcttcc
    17701 tttccattaa caaactttcg cagtaaatcc catgacacag acagaatcag cgattctggc
    17761 gcacgcccgg cgatgtgcgc cagcggagtc gtgcggcttc gtggtaagca cgccggaggg
    17821 ggaaagatat ttcccctgcg tgaatatctc cggtgagccg gaggcgtatt tccgtatgtc
    17881 gccggaagac tggctgcagg cagaaatgca gggtgagatt gtggcgctgg tccacagcca
    17941 ccccggtggt ctgccctggc tgagtgaggc cgaccggcgg ctgcaggtgc agagtgattt
    18001 gccgtggtgg ctggtctgcc gggggacgat tcataagttc cgctgtgtgc cgcatctcac
    18061 cgggcggcgc tttgagcacg gtgtgacgga ctgttacaca ctgttccggg atgcttatca
    18121 tctggcgggg attgagatgc cggactttca tcgtgaggat gactggtggc gtaacggcca
    18181 gaatctctat ctggataatc tggaggcgac ggggctgtat caggtgccgt tgtcagcggc
    18241 acagccgggc gatgtgctgc tgtgctgttt tggttcatcg gtgccgaatc acgccgcaat
    18301 ttactgcggc gacggcgagc tgctgcacca tattcctgaa caactgagca aacgagagag
    18361 gtacaccgac aaatggcagc gacgcacaca ctccctctgg cgtcaccggg catggcgcgc
    18421 atctgccttt acgggga
//
LOCUS       DAJOWB010000091        18387 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJOWB010000091 DAJOWB010000000
VERSION     DAJOWB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.091"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            876..1532
                     /gene="pphA"
                     /locus_tag="OHO85_003391"
     CDS             876..1532
                     /gene="pphA"
                     /locus_tag="OHO85_003391"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCQ2672726.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(1533..1724)
                     /locus_tag="OHO85_003392"
     CDS             complement(1533..1724)
                     /locus_tag="OHO85_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCQ2672727.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLHSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(1829..2065)
                     /locus_tag="OHO85_003393"
     CDS             complement(1829..2065)
                     /locus_tag="OHO85_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCQ2672728.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(2183..3622)
                     /gene="rsmF"
                     /locus_tag="OHO85_003394"
     CDS             complement(2183..3622)
                     /gene="rsmF"
                     /locus_tag="OHO85_003394"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCQ2672729.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGVAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCMWLKETYPDAVEFLPLGE
                     LFPAANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPTYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(3702..6335)
                     /gene="yebT"
                     /locus_tag="OHO85_003395"
     CDS             complement(3702..6335)
                     /gene="yebT"
                     /locus_tag="OHO85_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCQ2672730.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIASWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(6304..7587)
                     /gene="yebS"
                     /locus_tag="OHO85_003396"
     CDS             complement(6304..7587)
                     /gene="yebS"
                     /locus_tag="OHO85_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCQ2672731.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7717..8214
                     /gene="msrC"
                     /locus_tag="OHO85_003397"
     CDS             7717..8214
                     /gene="msrC"
                     /locus_tag="OHO85_003397"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCQ2672732.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDINW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHVFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8311..9009
                     /gene="proQ"
                     /locus_tag="OHO85_003398"
     CDS             8311..9009
                     /gene="proQ"
                     /locus_tag="OHO85_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCQ2672733.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAATAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            9029..11077
                     /gene="prc"
                     /locus_tag="OHO85_003399"
     CDS             9029..11077
                     /gene="prc"
                     /locus_tag="OHO85_003399"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCQ2672734.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            11269..12150
                     /gene="htpX"
                     /locus_tag="OHO85_003400"
     CDS             11269..12150
                     /gene="htpX"
                     /locus_tag="OHO85_003400"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCQ2672735.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(12196..13569)
                     /locus_tag="OHO85_003401"
     CDS             complement(12196..13569)
                     /locus_tag="OHO85_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2672736.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            13746..14537
                     /gene="kdgR"
                     /locus_tag="OHO85_003402"
     CDS             13746..14537
                     /gene="kdgR"
                     /locus_tag="OHO85_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCQ2672737.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(14680..14919)
                     /locus_tag="OHO85_003403"
     CDS             complement(14680..14919)
                     /locus_tag="OHO85_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672738.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            15078..15221
                     /gene="mgrB"
                     /locus_tag="OHO85_003404"
     CDS             15078..15221
                     /gene="mgrB"
                     /locus_tag="OHO85_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCQ2672739.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            15296..15583
                     /locus_tag="OHO85_003405"
     CDS             15296..15583
                     /locus_tag="OHO85_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCQ2672740.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            16252..16395
                     /locus_tag="OHO85_003406"
     CDS             16252..16395
                     /locus_tag="OHO85_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCQ2672741.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            16408..16617
                     /gene="cspE"
                     /locus_tag="OHO85_003407"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             16408..16617
                     /gene="cspE"
                     /locus_tag="OHO85_003407"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCQ2672742.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            16783..17592
                     /gene="rlmA"
                     /locus_tag="OHO85_003408"
     CDS             16783..17592
                     /gene="rlmA"
                     /locus_tag="OHO85_003408"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCQ2672743.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(17589..18155)
                     /gene="mntP"
                     /locus_tag="OHO85_003409"
     CDS             complement(17589..18155)
                     /gene="mntP"
                     /locus_tag="OHO85_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCQ2672744.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4829 a         4396 c         4657 g         4505 t
ORIGIN      
        1 tctttttcag ccctatggtt cagttaaatt tgattgttat cgtacgaagg tttgaacgtg
       61 tgtgtgcgta tgcggtattt tttcttgcga gtgccggagt tatgagagcg ttgtattaac
      121 cttcataatc tgacctattc aattgattcc agtacaccaa tgggtatttt ggtgttatgg
      181 tatgtgactc caaataaaat ggagcgtgaa ctgatagttg tgcgaacaaa agtcggacta
      241 gaaaccgctc gggtacagtg gcgaattgga gttggcgacc caaactgacc taggaacaat
      301 cgttgcagat cggacgatta atttcatcag aaattcctcg ctaaaaggtg gctatcactg
      361 tgatgttaac gtatctaatt tgtacaagaa atttctgcgg gggaaaatga aaccgcagcc
      421 acgtcgtatg caagaacgtg ccacggctgg ctgatggacg ttcgatagcg cgagtttgag
      481 tgaaaatcat cctgaggtga ttttacataa ttgttgcaga atcattcaat atagggattg
      541 cttatttctg cctggattgg tctgaaatag tcccgaaatt ttctctaaaa aactcgaaaa
      601 aaatggtaac taattgaatg cattaatatg caatggtacg tgtttgggat taaaagatga
      661 gcgtaacttt gcttaacaca ttaatttaaa aagggtttct tgtttgttga cgaaaacagg
      721 aatcgtgttc ggtctctttt tatctgttaa aagccagaag catttccttc gctgacttta
      781 tagtcaacca taacacacac tctactgtct gagtccagcg ttttttaaca ttcttgttaa
      841 gattatgtga tctttagcgc gggaggaaaa tattgatgaa acagcctgcg cccgtttatc
      901 agagaattgc gggtcatcaa tggcgacata tctggctttc tggcgatata cacggttgtc
      961 ttgagcagtt gcgccgcaaa ttatggcatt gtcgttttga tccgtggcga gatttactta
     1021 tctcagtggg agacgttatc gatcgtgggc cgcaaagttt acgttgtctg cagttactgg
     1081 aacaacattg ggttcgtgcg gtaagaggca atcatgaaca gatggcgatg gatgcgctgg
     1141 catcccagca gatgtctttg tggttgatga atggcggcga ctggtttatt gcgctggcag
     1201 ataatcaaca gaaacaagcg aaaacggcgc tggaaaaatg tcagcatttg ccctttattc
     1261 ttgaagtaca cagccgcacc ggcaaacatg ttattgctca tgccgattat ccagatgatg
     1321 tttatgaatg gcaaaaggac gttgatttgc atcaggtctt gtggagccgc tcacgattag
     1381 gtgaacgccc aaaagggcag ggaattacag gtgctgatca tttctggttt ggtcatacac
     1441 cgttgcgaca tcgcgtggat attggcaacc tgcattatat tgataccggt gctgtctttg
     1501 ggggcgaact gactcttgtg caattgcaat aattaaaaat caccgtactc ctgtgcaggt
     1561 cgccagaaac catctataaa atcctcaatc ggaaaacaac cgccatggcg gatccgttga
     1621 tcgctcatag aatgaagaca ctgctgttcc gtgttgtaga catccacaac aatatcttca
     1681 caaccgccat ccaggtagca aacaaaaagt accagcgcga acatttcatc cccgaagtgt
     1741 ggtgccgtac cgttaagttt aggagagatt ttacaacggg ggaataacca ggacaaataa
     1801 cccgccatgc cgacgggttc tttttggatc aggcaagacg cataatccag gcatcagatt
     1861 gttgatcgta acgggtacgg taaaggacag agtgttcgcc atttagcagg gcagggatac
     1921 tggtttcagc atcccaggca tccagttgca tacataaatc ggggtcagat ttacaaggaa
     1981 tggttaacgt tcgatcaccc ggcgattcac cgtgtaattc gccgtcgtcg atttcaaaag
     2041 cgcctatgcg cacactggtt ttcatcttcg tgctctccat ttagctgccg attgtggtac
     2101 gaccagtccg atcgcaccat ttttcagcct agttgaggcc gccgaatgtg ccagtcaaaa
     2161 agtgcgcttt ttttgtgcgc tgtcaggcgt taccggtaaa aagtttccca tcgcgcacca
     2221 gttcacgcgg atagctgttt ttcaatcgcg aaccaatccg tttggctaaa ccaatcggtt
     2281 gatgctggaa agtaaccaat acatcatccg ccactggcgc ggcttgcggg taaacatcgc
     2341 gcccgcgata ccactcctcc gcttcctgcg gtgtcagctc gaatgcattc atattatcgg
     2401 gtgaggcaag agcaataacc gcttcatgct gccagcgata acctttgttg tgcgtctcgg
     2461 caagtttaat ccccaaccga gaaaatcgga ctttaccgat cagggcttca atgcccaccg
     2521 ggaacaacca cagttctttg tcacgctgcc agaggcgcag gttttcatcc cagtttaagc
     2581 caacacctgc agccgcctgc cgaatttgtc cagcttcgcg atctttcacc gggctgaacg
     2641 gaaaattgcc cactttgtat gttggggtgg gtaaggcggg aatggcctga gttttacgca
     2701 gacgagcaac gaagaagcct tcgcagtcgt aaatttgtgg gaaaacatgc aaaaagcctt
     2761 cttcggtcag cgctttgttt gcagcaggga agagctcgcc aagtggtaaa aactctactg
     2821 cgtcagggta agtctctttc agccacatgc aaacggcttc gttttcttcc ctgtttaagg
     2881 tacaggtcga gtaaaccagc gtaccaccag gacgtaatgc atgaaaggcg ctgtcgataa
     2941 gctcccgttg tgtagctgcg atttcctgat tgctttctgg tgaccagttt tttagcgcat
     3001 cgggatcttt acgcaccacg ccttcgccag agcagggagc gtccagcaaa atggcatcga
     3061 acatttctgg cactgccaca ccaaacacgc ggccatcaaa atgtgtgagc gcaacattac
     3121 tgatgccaca gcggctgata ttggcatgta acacttttac ccgactggcg gaaaactcat
     3181 tggcaaggat tgccccttcg ttattcatcc gtgcggcaat ttgcgtcgtt ttggagcctg
     3241 gcgcagcagc gacatccatc acccgctgtg gtgcattacc gtcagcaaac aaggcggcga
     3301 cgggcaacat tgaactggct tcctgaatat aaaacaggcc gcttaaatgc tcggcggtac
     3361 tacccaatgg caatgcatct tcattgtcgc gttcaatcca gaaaccttct tcacaccacg
     3421 gaattggcgt aagcgtccag ccataaggag cggttaattg caggaaatca gcaacggaga
     3481 ttttcagcgt attaacgcga atgctgcggc gcaacgggcg ctgacaggcg gcaagaaaat
     3541 catcaaaaga gagcgtcgaa ggcatcgctt cgcgcatttg cgtcagaaag gcgtccggga
     3601 aataaacggt gtgttgggcc acgagcatat accactggca aaaaaacagg cgcgcagttt
     3661 agcataaacg ctccggcgca ggcacgccgg agcagtgggc attatttggg aagcgcagta
     3721 ccccattcac gccactcttt cggttcactt tcctgcaaca ggaagtgctt gccttcctgg
     3781 gctttcggtg ccagtggcgt ccccggaggc gtggcgaagg cgatgccgcc acggataaac
     3841 tggttaaagg tgccggtttt cactacgccg cccgtcagac caaagtccag actgtaaccc
     3901 gatgccaacc agaagacgga attgttacgc accaggtgtt gatagcgttt actgatgcgc
     3961 atcgcaatca tcacgcgatc tgacaatgtc cccagcgtca ttcctgtaac cgtaccgact
     4021 tccagaccac ggaacagcac aggcgtaccg atgcctaacg aaccggcttc cggcgcttca
     4081 acaataatgc ttaagccatc caggtaacgc gaatcagtaa tggtggcctc ttgtaattca
     4141 aagtcgcggc gaggattgcc ccggcctggt tcgacgttga tatacggctg gaggatagta
     4201 tcaagatgct caacgccagc tgccgaaatt tgcggtgtga ccactgagaa gcgcgtacca
     4261 ccgcgagcaa aggtctggac atattccgga tagagcaccg cctttgcctg tacttcattg
     4321 cgcgcggtaa tcagatccag cgtctggatt tgcccgatat caataccgag atagcgaatt
     4381 ggcatcccga ccgccagttt tccggcatcg aaagcgtgaa gcgtaatctg cccgccaacc
     4441 gcacgagccg ctgtttcgga agcatacaga attcgtttgt cgcctttacg ctgactggcg
     4501 ctggcaccgc tgaggttatc gaagctaatg gctcccttta atgctctgga gagcggggat
     4561 gcctgtacgg tcaggccact accattcagc tgaactttcg ccccgccttc tgcccagaac
     4621 acgctattgc tggtcagaag gttgcgatac tccggcttaa tatgcagatc gatatcaaac
     4681 gcgttagctc gcggacgcac ggtaataact tcaccaactt caaatttacg gtacagcact
     4741 accgatcctg cctgcacatc cggcagcgtc tctgcactca aactcacggt tgtggtgggt
     4801 aaatcgctaa ggctgttctc cagcgctttt tccagattgg catacagtgg atagctggct
     4861 ttcatctcgc ctttatcgcc cggcagaata cgtatcccgc cgttaatcca ttctgaggcg
     4921 ctggctccga gaaactcaac gccatccagc cccaccttca cgtcgacacg gctgttgacg
     4981 acaaatttgc tatcgccttt taccagttcg cgatgctgag gctcaatggc gacggtaaag
     5041 gtaacgcctt tgctggtgag tttacgatca ataacctggc ctacctgcac gccgtgaaga
     5101 atgagcggct gaccagcatc aataccgtaa ctttccggtg ctgtcagggt cagtgtcaga
     5161 acgtccggct cttgcagcag ggctttttcg cccggcacaa caacgaactc tttgcgtggc
     5221 tcgccatcgc cgggcaccag ctcgaaggtt ttgccggtca gcagggcgct gagattagca
     5281 tcgctaaggg ataatttcgg gttgcgtaat tcgatgcggg tattttcacg aagcagggta
     5341 acgacgctgg gatcaacggt catttcccct gtgactttac caccaggatt taaatccagt
     5401 ttagtcagct gtccgacttc cagcccctga tacattaacg gcgtcgagtc ggcggttaat
     5461 ccggccccac tcggcagttc cagttttatt attacgccac gctggctgtg ggccagatct
     5521 tcatacagac caaaggtatc ttccgcctcg gcaggtttcg actcttctgg tgaatcgaag
     5581 gcaatcgcac cgttaaccag tgccgccagg ctttccagtt tcaccttcgc gccactgata
     5641 ctgacgttgg catcaacgcc ggaaacgttc cagaaacggc tacctttttt caccagatcg
     5701 gtaaaacgcc gctcgatcag gacatcaatc accacgcctt gcttgttggg attgatggca
     5761 tagtcgtaga cttttcccac cgggatcttg cggaaataga ccaatgaacc gctgttcagc
     5821 gaaccgagat cgggggcttg caggtggatc atcagatcgc cattgtccag ccgatatttc
     5881 ggttgggtat cgagtgcgac aaagtgatcc tgctctttac ctttacccgg catcatgccg
     5941 atatagttcc caccgacgag ggcgtccagc ccggagacac ctgccaacga tgcttttggc
     6001 gtcaccagcc agaactgagt ctcttcgcgc agcgcatctt tcatatcgga cttgatgctg
     6061 accttgactt caatcttacg aagatcgtcg ctgaggctga tatcctgcac tgttccgact
     6121 tcaacgccct gataacgaac aggcgtacgg cctggaacaa taccatccgc cgacataaag
     6181 tcgatggtga cggtattacc ccggtcctga taactgtccc aaatcagcca actggcaatc
     6241 attagcgcga tgaacggcag cagccagaaa ggtgagatac ggcgtttatt tttaatctgc
     6301 gcttcagtcg tcgaagcggg cgtttcctga ctcatgtgca tcccaaagta agcggctgtc
     6361 cagccattcc acagcaagaa tagtcaaaat taccgctgcg ccgaaataaa acgcagccgg
     6421 tcccatagta aaagcgagga tctgatcgcg attaatcagc gacatggtta aagatatgac
     6481 aaacaggtcg agcatcgacc agcgaccaat ccaggtcacc atccgcagta acagaatgcg
     6541 tgtgcgtaaa ccttgctggc atttaaaatg aatgctcaac agtaaagtga acatgacgat
     6601 cactttagta aacggtacca gaatactggc gataaacacg attcctgcaa ccgcaatgtt
     6661 gctacttgcc agcgacataa ttccggaaag aattgtatct tcctgccgtc ctccgttcag
     6721 ataaatgata gaaataggca acaggttggc aggtaacaat aaaacgattg acgctaacag
     6781 cgccgcccag catttttgca gactatgacg gcgacgcagg cgtagcggga tatggcaacg
     6841 cgggcagcga ccacgctgat ctggatagcc ggtaaaatgg cacccaagac agacacgaag
     6901 tttctcgtcc ctacgcgtag cggggcgctg cggataaaat cgctcccaca gttcttcgac
     6961 attaagatgt gacaacgtca ccgtcgttaa aatcaccaac gccacaaaag agaacaagcc
     7021 gacaccagcc tggatatggg cataatcctg tacctttata gaagcaacgc caatgccgac
     7081 caggtagata tcgagcatta cccactcttt aagtcgctca agcatcagca gtaccggacg
     7141 taaattcatt cccagtcggt taccaaacca taaataagct atggaggtca ccagaatgag
     7201 cggggcacca ataacgcaga aaaagaccat cgaccccgtt atcgcatcgc cctgtttggt
     7261 catttgccag atgccttgca taacgttggc gtcaatacga atgcctaaca gccagatatg
     7321 caacagcggt tcgccccagg caaacggcat aagcaacagc atggtgaagg ccattgccgc
     7381 caggcgcgtt agcgaccagt cgcgcccgtc acgaattttt gcctgacagc gcggacaata
     7441 ggcactttga tgagaattta tctcgggcag gctaaacagc atgtcacatt gcgggcaacg
     7501 ttggtaatca ccacgcggca gttcctcgcc gattgccctc actgttatct tttttgtcgg
     7561 cgtaatttgt ggtgtgttaa gagccatgac gtgccaaatg taataagaat taacattaat
     7621 cttaacccat gatgggaatg atcttgagca ttaacgcatt taatgcttat tttaataggc
     7681 tggcagatcc aggtaagaca accaagtgat tatataatga acaaaacaga attttacgcg
     7741 gatttaaatc gcgactttaa cgcgctgatg gcgggagaaa ccagttttct ggcaacgctt
     7801 gcgaacacca gtgcgttgtt atatgagcgt ctcactgaca taaactgggc aggtttttat
     7861 ttgcttgagg acgatacact ggtactcgga ccatttcagg gcaaaattgc ctgtgtccgg
     7921 atacccgtcg ggcgcggcgt gtgcggcact gcggttgccc gcaatcaagt gcagcgtatc
     7981 gaggatgttc atgtgtttga cgggcatatt gcctgtgatg cggcgagtaa ttctgaaatt
     8041 gttctgccgc tggtggtgaa aaatcagatt attggtgttc tcgacatcga tagtaccgtc
     8101 ttcggtcgct ttacagacga ggacgagcaa ggcttacgtc agcttgtggc acagcttgaa
     8161 aaagtgcttg caacgacgga ttacaaaaaa ttctttgcga gcgtcgcagg ataatcaacg
     8221 gataacgtag catttactga tagcgtcatt ataatgacgc ctgttcatgc ctgcgcttgt
     8281 tggctacgtc cgttgtaatc aggaaatttc atggaaaatc aacctaagtt gaatagcagt
     8341 aaagaagtaa tcgcgtttct ggccgaacgt tttccccact gtttcagtgc ggaaggtgaa
     8401 gcgcgtccgc tgaaaatcgg tatttttcag gatttggtcg atcgtgttgc tggggaaatg
     8461 aacctgagca aaacgcaatt gcgatccgct ttacgtctct acacttcgag ctggcgttat
     8521 ctttacggtg ttaaacccgg cgcaacgcgt gtcgatcttg acggcaaccc atgcggtgag
     8581 ctggacgagc aacatgtaga gcatgctcgc aagcagcttg aagaagcgaa agcgcgtgtt
     8641 caggcacagc gtgctgaaca gcaagcgaaa aaacgcgaag ctgccgcaac tgctggtgag
     8701 aaagaagacg caccgcgccg cgaacgcaag ccacgtccga ctacgccacg ccgcaaagaa
     8761 ggcgctgaac gtaaacctcg tgcgcaaaag ccggtagaga aagcgccaaa aacagtaaaa
     8821 gcacctcgcg aagaacagca caccccggtt tctgacattt cagctctgac tgtcggacaa
     8881 gctctgaagg tgaaagcggg tcaaaacgcg atggatgcca ccgtattaga aatcaccaaa
     8941 gacggcgtcc gcgtccagct gaattcgggt atgtctttga ttgtgcgcgc agaacacctg
     9001 gtgttctgaa acggaggccg ggccaggcat gaacatgttt tttaggctta ccgcgttagc
     9061 tggcctgctt gcaatagcag gccagacctt cgctgtagaa gatatcacgc gtgctgatca
     9121 aattccggta ttaaaggaag agacgcagca tgcgacggta agtgagcgcg taacgtcgcg
     9181 cttcacccgt tctcattatc gccagttcga cctcgatcag gcattttcgg ccaaaatctt
     9241 tgaccgctac ctgaatctgc tcgattacag ccacaacgtg ctgctggcaa gcgatgttga
     9301 acagttcgcg aaaaagaaaa ccgagttagg cgatgaactg cgttcaggca aactcgacgt
     9361 tttctacgat ctctacaatc tggcgcaaaa gcgccgtttt gagcgttacc agtacgcttt
     9421 gtcggtactg gaaaagccga tggatttcac cggcaacgac acttataacc ttgaccgcag
     9481 caaagcgccc tggccgaaaa acgaggctga gttgaacgcg ctgtgggaca gtaaagtcaa
     9541 attcgacgag ttaagcctga agctggcagg aaaaacggat aaagaaattc gtgaaaccct
     9601 gacacgccgc tacaaatttg ccattcgtcg tctggcgcaa accaacagcg aagatgtttt
     9661 ctcgctggca atgacggcgt ttgcgcgtga aatcgacccg cataccaact atctttcccc
     9721 gcgtaatacc gaacagttca acactgaaat gagtttgtcg ctggaaggta ttggcgcagt
     9781 gctgcaaatg gatgatgact acaccgttat caattcgatg gtggcaggtg gtccggcagc
     9841 gaagagtaaa gctatcagcg ttggtgacaa aattgtcggt gttggtcaaa caggcaagcc
     9901 gatggttgac gtgattggct ggcgtcttga tgatgtggtt gccttaatta aagggccgaa
     9961 gggcagtaaa gttcgtctgg aaattttacc tgctggtaaa gggaccaaga cccgcactgt
    10021 aacattgacc cgtgaacgta ttcgtctcga agaccgcgcg gttaaaatgt cggtgaagac
    10081 cgtcggtaaa gagaaagtcg gcgtgctgga tattccgggc ttctatgtgg gtttgacaga
    10141 cgatgtcaaa gttcaactgc agaaactgga aaaacagaat gtcagcagtg tgatcatcga
    10201 cctgcgtagc aatggcggtg gggcgttgac cgaagcggta tcgctctcag gtctgtttat
    10261 tcctgcgggt cccattgttc aggtccgcga taacaacggc aaggttcgtg aagacagcga
    10321 taccgacggg caggtgttct ataaaggccc gctggtggta ctggttgacc gtttcagtgc
    10381 ttcggcttca gaaatctttg ccgcggcaat gcaggattac ggtcgtgcgc tggttgtggg
    10441 tgaaccgacg tttggtaaag gcaccgttca gcaataccgt tcattgaacc gtatttacga
    10501 tcagatgtta cgtcctgaat ggccagcgct gggttctgtg cagtacacga tccagaaatt
    10561 ctatcgcgtt aacggcggca gtacgcaacg taaaggcgta acgccagaca tcatcatgcc
    10621 gacgggtaat gaagaaacgg aaacgggtga gaaattcgaa gataacgcgc tgccgtggga
    10681 tagcattgat gccgcgactt atgtgaaatc aggcgattta acggcctttg ggccggagct
    10741 gctgaaggaa cataatgcgc gtatcgcgaa agatcctgag ttccagaaca tcatgaagga
    10801 tatcgcgcgc ttcaacgcta tgaaggacaa gcgcaatatc gtttctctga actacgctgt
    10861 gcgtgagaaa gagaataatg aagatgatgc gacgcgtctg gcgcgtttga acgaacgctt
    10921 taaacgcgaa ggtaaaccgg agttgaagaa gctggatgat ctaccgaaag attaccagga
    10981 gccggatcct tatctggatg agacggtgaa tatcgcactc gatctggcga agcttgaaaa
    11041 agccagaccc gcggaacaac ccgctcccgt caagtaatat caatcaggca caagaaattg
    11101 tgcctgattt tttaacagcg gcaagatgcc gtaaatcaga tgctacaaaa tgtaaagttg
    11161 tgtctttctg gtgacttacg cactatccag acttgaaaat agtcgcgtaa cccatacgat
    11221 gtgggtatcg catattgcgt tttgttaaac tgaggtaaaa agaaaattat gatgcgaatc
    11281 gcgctcttcc tgctaacgaa cctggccgta atggtcgttt tcgggctggt actgagcctg
    11341 acagggatac agtcgagcag cgttcagggg ctgatgatca tggccttgct gttcggtttt
    11401 ggtggttcct tcgtttcgct tctgatgtcc aaatggatgg cattacgatc tgttggcggg
    11461 gaagtgatcg agcaaccgcg taacgaaagg gaacgttggc tggtcaatac tgtagcaacc
    11521 caggctcgtc aggcagggat cgctatgccg caagtggcta tctaccatgc gccggacatc
    11581 aacgcttttg caaccggtgc gcgccgtgat gcctctctgg ttgctgtcag caccggtttg
    11641 ctgcagaaca tgagcccgga tgaagccgag gcggtaattg ctcacgaaat cagccacatc
    11701 gccaatggtg atatggttac catgacgctg attcagggcg tggtgaacac cttcgttatc
    11761 tttatttccc gtattctggc gcagcttgcc gcgggtttta tgggcggaaa tcgtgatgaa
    11821 ggtgaagaga gcaacggcaa cccgctgatc tactttgcgg ttgcaacggt tctggaactg
    11881 gtgtttggta ttctggcgag cattatcacc atgtggttct cgcgtcatcg tgaattccat
    11941 gctgatgccg gttcggcaaa actggttggt cgcgagaaaa tgattgccgc gctgcagcgc
    12001 ctgaaaacca gctatgaacc gcaagaagca accagcatga tggctttctg cattaacggt
    12061 aagtcgaaat cgctcagtga gttgttcatg actcacccgc cgctggataa acgaattgaa
    12121 gctctgcgta cgggtgaata cctgaagtaa tatgaagtaa atactaaaaa gcgcgtcctg
    12181 atcgacgcgc tttttttatg ccctggatcg tggctgagtg atacgtaaac cactgacaca
    12241 ggcagcaatc actgccagaa tagccgcagc catcagcgaa acgtgcgtac cattatcacc
    12301 gaactgattt agcatcagcg ccaccagtgc cgcgccgcta ctctgaccca gtagacgagc
    12361 cgttcctaac atgccactgg ctccaccgct acgttcgcga ggagcggagg taataatggt
    12421 gtggttattg ggtgactgga acaagccaaa tccagcgcca cataagatca tcggccagat
    12481 aatattgata tccgcaggtg acgcgggcag cagaaccagg gaaaaaagcc ccgcagccat
    12541 gatgaacaac cctagagcac ccagcaatcc tgcatgtacg cgttcaatca aatagcctgc
    12601 caacggagcc atcaccatcg ttgctaacgg ccacggtgtc agaagtaaac ctgtttcgac
    12661 ttcactacgc ccgagcacgg tttgcaggta aaagggcagg gaaaccattg ccagcatttg
    12721 tgcgcagaaa gagcaaacag atgtgcaaat agaaagtgaa aacagcggga tacgcagtaa
    12781 atccaccggt agcagcggta cgggaagaga aagctggcgg cgaataaaga aaataccaac
    12841 aacaaccatt accaccagtt ccgcagcaat taacgtcagc gattgcccct gagcgaaacc
    12901 actcaacgca gtgataagca ggccgaaggt taacgcgttc atcacggcgc tgggcaggtc
    12961 gaaacggggt ttactggcgc gagaaccatt gggtggcaga aaacgcatcg ccagaagcag
    13021 ggcgataata cctaacggta cgttgattaa aaataaccat ttccaggatg cgatggagag
    13081 gattgctgca gcaattgtcg gcccggcagc agaagagacg gcaacaataa acgagtttat
    13141 gcccatccct ctccccagaa aacgttgtgg atagatcagg cggataagtg cggtattaac
    13201 gctcatcaac gctgcaccgc cgaaaccttg tatgacacgc gcaagggtga gcatttgcag
    13261 cgaatcagaa agggcgcaga acagtgaaga caacagaaaa acgaccagac cgcatttata
    13321 aatacgtcga tagccaaaca tatcgcccag aaacgaaaac gagagcaggg agatgacaat
    13381 ggcgatttga taggcgttca ctacccagat ggaactggct ggcgtggcat gaaggtccgt
    13441 ggcgattgtt ggcagggcga cgttggcgat tgcgccgtca aggacagcca tcgaaatacc
    13501 aatcacaatg gttaatatcg caccgtatcg ctggggcaat ggcaggccgt cggcctgaac
    13561 ttttggcata ggaattttat atctttggtg aataatcaac aggggtatga ttctagcact
    13621 gatattcacg gtggatgtcg cagaattgta actaaccggc aaaagatgga ttgcgaccac
    13681 cctggtgcga aattataata aaaaacagtt ctgattttta taaaacactc gcaatgaggt
    13741 gataaatggc taacgcagat ctggataaac agcctgattc tgtatcttcc gtgctaaaag
    13801 tttttggcat tttgcaggcg ctgggtgaag agcgcgaaat agggataacc gagctgtcgc
    13861 agcgcgtcat gatgtcaaaa agcaccgttt atcgcttttt acagaccatg aaaaccttag
    13921 gttatgtggc gcaggaaggg gagtcggaga aatattccct gaccctgaaa ttgtttgaac
    13981 tgggcgctcg cgcgttacaa aacgtcgatt taattcgtag cgcagatatc cagatgcgtg
    14041 agatctcccg cctgaccaaa gaaactatcc acctcggcgc actggacgaa gacagtattg
    14101 tttacattca caaaattgac tctatgtaca atttgcgcat gtattcacgg attgggcgtc
    14161 gtaatccgct gtacagcacc gcgattggta aggtactgct ggcatggcgc gatcgcgatg
    14221 aagtgaagca aattcttgag ggcgtggagt ataaacgcag taccgagcgg accatcacca
    14281 gtacagaagc gttattaccc gttctggacc aggtgcgcga gcaggggtat ggcgaagata
    14341 acgaagagca ggaagaaggg ctgcgatgca ttgcggtacc ggtatttgat cgctttggcg
    14401 tggtcattgc cggtttgagc atctccttcc cgacgttgcg tttctctgaa gagcgtttac
    14461 aggaatatgt cgcaatgttg cataccgcag cgcgcaaaat ttctgcccaa atgggttatc
    14521 acgactatcc gttctgatga gagtaagaac ctgtcggaat atcaaacaga caggttcttt
    14581 atttagcatg agaaaaataa agttgaaggt gcgttatatt aaacgcgctt gctataagaa
    14641 tattttactc atgagtgaga atctggttat ttattaccct taaccattat cgaccacgat
    14701 attgctttta cgtaacagtg ggcaatctgt tatccccaag aaaccacttt tagtgtgcaa
    14761 gtattgtacc gtgctggtgc ctctggcagt cagataggta cattgcaaac ctaatcctgc
    14821 ggcattctct ttgcttccaa tcaaaacgcc atatccgctg agtaataatc ctatccatac
    14881 cagtgctatc agcataactg tgcgaatgat gaatcgcatt acaacctctt ctctttttat
    14941 gttcgcttaa tcgtagcggc aatatgcgct gaagcaagct actcattccg aaaaagcacg
    15001 aatatcgaca tagttaggcg ctgtttaact aacgcatgct agtttaatga cataaggtag
    15061 gtgaagcgga gattggagtg aaaaagtttc gatgggtcgt tctggttgtc gtggtgttgg
    15121 cttgcttgct gctttgggcg caggtattca acatgatgtg cgatcaggat gtacaatttt
    15181 tcagcggaat ttgtgccatt aaccagttta tcccgtggtg atatcatttt ttcagcacta
    15241 gattttcttc ctctccagca gtggtagaat agcgcctcta ctcagaggtg gtgaaatgaa
    15301 cgaagttgta aattcaggcg tgatgaacat tgcgtctttg gttgtatcgg tggtggttct
    15361 tcttatcggg ctcatcttgt ggttttttat caatcgtgcc agttctcgga ctaacgaaca
    15421 gattgaactg cttgaggcgt tgctggatca gcaaaaacgt caaaatgcac tgttacgtcg
    15481 tttgtgcgaa gcaaacgaac ctgagaaagc agataaaaag accgttgaga gtcaaaaatc
    15541 ggttgaagac gaagatatta ttcgtctggt cgccgaacga taatccttcc tcaatcttga
    15601 gtaaccccgt cgttgaaggg ttactcaatt catcctgatt atacgcactg ccatggtgca
    15661 actgcttcaa attaaaactc tggtctgact tcaaataatt atcatattat ttatttgcgt
    15721 aacgggatat cagaaaaaga tattttcctc attaatttat taggatgttt acatcggatt
    15781 tgtgattaag cgtggtatta tttattagcg aaacgtttct ctcttgattt tttgctcatt
    15841 catcaatttt tcttatttta aattttcaat ccctttgggg attgacttct ctttagggta
    15901 attgatagcc gttaactgac tgttttatga gaaaaagtga tataactttt tattcattgc
    15961 atagcaaaaa atgtgatatt gcacgcacta tgtaataact tctcccactg gcctggaaca
    16021 actgaactta ttgaactatg ttagaaacta cgccagttta agtatctgcc tgaactggca
    16081 aggttaagca caatgatata tcggcgcgta ttccgttgca taagtgtgca aaaaaagtgg
    16141 aagacgtatc gagatttgtg cgtctgatcg agacatgttt aaaaatggct tgccataatt
    16201 aacgttgtat gtgataacag atttcgggtt aaacgaggta cagttctgtt tatgtgtggc
    16261 attttcagta aagaagtcct gagtaaacac gttgacgttg aataccgctt ctctgccgag
    16321 ccttatattg gtgcctcatg cagtaatgtg tcagttttat ctatgttatg cctgcgggcg
    16381 aagaaaacaa tctaaggaat ttttcaaatg gcaaagatta aaggtcaggt taagtggttc
    16441 aacgagtcta aaggttttgg cttcattact ccggctgatg gcagcaaaga cgtgttcgta
    16501 cacttctccg ctatccaggg taatggcttc aaaactctgg ctgaaggtca gaacgttgag
    16561 ttcgaaattc aggacggcca gaaaggtccg gcagctgtta acgtaacagc tatctgatcg
    16621 aatccactga tctgaagtgt gaatacgctt caatctcgct ataaagcctc gtcgaatgcg
    16681 aggcttttta ctatgcttta tcttcgctcc tggcgttcgg atatttgccc gccgcgtgat
    16741 tcgcgttaca cttgcggcct ttagtatcct gccggagttg tcatgtcttt ttcctgtcca
    16801 ctttgccatc agcctctttc gcgtgaaaaa aacagctata tctgtcccca gcgacatcag
    16861 tttgatatgg cgaaagaagg gtatgtcaat ctgctgcccg ttcagcataa acggtctcgt
    16921 gatccgggcg acagcgcgga aatgatgcaa gcacgccgcg cattcttaga tgccggacat
    16981 tatcagccgc tgcgtgatgc aattgtcgcc caactgaggg aacggcttga tgataaggcc
    17041 acggcggtgc tggatattgg ctgtggtgaa gggtattaca cacacgcatt tgccgatgcg
    17101 ttgcccgaaa tcaccacgtt tggtctggat gtttcgaagg tagcgataaa agcggcggcg
    17161 aaacgctatc cgcaggtcac tttttgtgtc gcttccagcc accgtttgcc gttttccgat
    17221 accagtatgg acgccataat acgtatttac gcgccgtgta aagcagaaga attagcacga
    17281 gtagtgaagc ccggcggctg ggtcattact gccacgccgg gaccgcgaca tttgatggag
    17341 ctgaaggggc tgatttacaa tgaagtacat cttcatgcac ctcatgcaga acaactggaa
    17401 ggttttacat tacagcagag tgcggagttg tgttatccga tgcgtcttcg cggtgatgaa
    17461 gccgtcgcat tattgcagat gacgccgttt gcctggcgtg cgaagccaga agtctggcaa
    17521 acactggcag caaaagaagt gttcgactgc cagacggact ttaatattca cctctggcag
    17581 cgttcttatt aaccgtggaa gtgcgtccag aggatctgga cgccgatgcc gatcagtacc
    17641 agcccgccga gaatttccgc ttttttccca ataattgagc cgataaagcg accaaccatc
    17701 atccctaatg ttgacataat caaggttgca caaccaatgg ccaatgcggt cgcgataatg
    17761 ttgacctgca ggaaagcaag accaacaccc acagccatgg catccaggct ggtggcaatc
    17821 gcggtggtta ccagtagcca gaaaccgtgt cgacggcgcg gctcttcatc ttcatcatct
    17881 gcgccacgaa aaccctcaat aatcattcgc ccgccgagga atatcagcag cacaaacgca
    17941 atccagtggt tccattcaag gacaaaccgg ctggctaaca tgcccattcc ccagccgatc
    18001 agcggcgtca gggtttcgac ggcaccaaaa ataaggccgg ttcgcaatgc ttcagaaaat
    18061 ttcggtttat ggagggtggc acctttaccg attgatgcag caaatgcatc catcgacata
    18121 ccaaacgcaa gaagaacagt agcagtgata ttcatgacaa tgtcctgacc ggggtatcca
    18181 taaaacacat catcgccccc agcaaacatg catacatgaa caacaagccg cgatggtttt
    18241 cgcgttcatg caggcatagc gattgatgtg catatggtct cgcctgactg aaagtattca
    18301 gtccgtacgt gccacgtttt gcaacgagta tgttgacacg tacatttccg gaatctggaa
    18361 atcggctact ccccaaatga gggcgca
//
LOCUS       DAJOWB010000092        18321 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJOWB010000092 DAJOWB010000000
VERSION     DAJOWB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.092"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            210..935
                     /locus_tag="OHO85_003410"
     CDS             210..935
                     /locus_tag="OHO85_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCQ2672745.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            893..1570
                     /gene="bglJ"
                     /locus_tag="OHO85_003411"
     CDS             893..1570
                     /gene="bglJ"
                     /locus_tag="OHO85_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCQ2672746.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1608..2396)
                     /gene="fhuF"
                     /locus_tag="OHO85_003412"
     CDS             complement(1608..2396)
                     /gene="fhuF"
                     /locus_tag="OHO85_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCQ2672747.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2537..2773
                     /locus_tag="OHO85_003413"
     CDS             2537..2773
                     /locus_tag="OHO85_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCQ2672748.1"
                     /translation="MLQRMLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2812..2898)
                     /locus_tag="OHO85_003414"
     tRNA            complement(2812..2898)
                     /locus_tag="OHO85_003414"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2862..2864),aa:Leu,seq:cag)
     gene            complement(2933..3019)
                     /locus_tag="OHO85_003415"
     tRNA            complement(2933..3019)
                     /locus_tag="OHO85_003415"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2983..2985),aa:Leu,seq:cag)
     gene            complement(3048..3134)
                     /locus_tag="OHO85_003416"
     tRNA            complement(3048..3134)
                     /locus_tag="OHO85_003416"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3098..3100),aa:Leu,seq:cag)
     gene            complement(3402..4433)
                     /gene="rsmC"
                     /locus_tag="OHO85_003417"
     CDS             complement(3402..4433)
                     /gene="rsmC"
                     /locus_tag="OHO85_003417"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCQ2672749.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4536..4949
                     /gene="holD"
                     /locus_tag="OHO85_003418"
     CDS             4536..4949
                     /gene="holD"
                     /locus_tag="OHO85_003418"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCQ2672750.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4918..5364
                     /gene="rimI"
                     /locus_tag="OHO85_003419"
     CDS             4918..5364
                     /gene="rimI"
                     /locus_tag="OHO85_003419"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCQ2672751.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            5379..6056
                     /gene="yjjG"
                     /locus_tag="OHO85_003420"
     CDS             5379..6056
                     /gene="yjjG"
                     /locus_tag="OHO85_003420"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCQ2672752.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            6147..7736
                     /gene="prfC"
                     /locus_tag="OHO85_003421"
     CDS             6147..7736
                     /gene="prfC"
                     /locus_tag="OHO85_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCQ2672753.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            8129..8734
                     /gene="osmY"
                     /locus_tag="OHO85_003422"
     CDS             8129..8734
                     /gene="osmY"
                     /locus_tag="OHO85_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCQ2672754.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            8861..9022
                     /locus_tag="OHO85_003423"
     CDS             8861..9022
                     /locus_tag="OHO85_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCQ2672755.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            9144..10217
                     /locus_tag="OHO85_003424"
     CDS             9144..10217
                     /locus_tag="OHO85_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCQ2672756.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            10214..10996
                     /locus_tag="OHO85_003425"
     CDS             10214..10996
                     /locus_tag="OHO85_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCQ2672757.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(11311..12174)
                     /locus_tag="OHO85_003426"
     CDS             complement(11311..12174)
                     /locus_tag="OHO85_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCQ2672758.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(12146..13696)
                     /locus_tag="OHO85_003427"
     CDS             complement(12146..13696)
                     /locus_tag="OHO85_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCQ2672759.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            13954..14733
                     /gene="deoC"
                     /locus_tag="OHO85_003428"
     CDS             13954..14733
                     /gene="deoC"
                     /locus_tag="OHO85_003428"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCQ2672760.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            14860..16182
                     /gene="deoA"
                     /locus_tag="OHO85_003429"
     CDS             14860..16182
                     /gene="deoA"
                     /locus_tag="OHO85_003429"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCQ2672761.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            16234..17457
                     /gene="deoB"
                     /locus_tag="OHO85_003430"
     CDS             16234..17457
                     /gene="deoB"
                     /locus_tag="OHO85_003430"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCQ2672762.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPARMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            17537..18256
                     /gene="deoD"
                     /locus_tag="OHO85_003431"
     CDS             17537..18256
                     /gene="deoD"
                     /locus_tag="OHO85_003431"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCQ2672763.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         4403 a         4651 c         4924 g         4343 t
ORIGIN      
        1 aatgtctcca ggctatttca ccaggttatc atttgatgct aaccttctga cacatgcagt
       61 ggagttgttg tgcagcagga gtatgctgat atgaaagtga atgctaagga taatttattc
      121 gcttaatcta ttaatttgct ggaaacattt aaggaatgaa tatcctgaat aaaccatcat
      181 gatttttaga cagtggatgt ggaggaaata tgttgccagg atgctgcaaa aatggaattg
      241 ttatcagtaa aatacctgtt atgcaagcag ggttaaaaga ggtcatgagg actcacttcc
      301 ctgaatatga aataatatcc agcgcctctg cggaggacct taccttatta caattacgtc
      361 gttccggatt agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg
      421 aacattatta ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac
      481 gctcctggta ttcccaggca gtagaattgc tcatgtgccc tacggcgacg ttattgtctg
      541 atgttgaacc cattgagaat ctggtcaaga ccgtacgttc cggcaatacg cacgcagagc
      601 gtatcagcgc catgctgacc tctccggcaa tgaatgaaac tcatgatttt agctatcgct
      661 ccgtcattct cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca
      721 tcaaccagat agcttcgttg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca
      781 gtgcgatgcg tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg
      841 cgcagggtgc aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca
      901 cagccgaatt aagaagagaa atgtcgcact catagaaaaa tgcgtcatga gcagtatcgg
      961 tattgagagt ttattcagaa agtttgcggg taacccttat aagctccata cctataccag
     1021 tcaggagtca tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt
     1081 ttctgccatg agaagtgagc gcagagaggg attatcttgc ctgactgaac tggcgattaa
     1141 gtttccgcgt acccggcgtt tagttattgc ggatgatgat attgaagctc ggctgattgg
     1201 ttcattgtcg ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt
     1261 tcatcaggaa ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa
     1321 tcagtggtac attaaccaaa gccggacgct aagcccgacg gagagagaaa tattgcgctt
     1381 tatgtcgcgt ggctactcaa tgacacaaat tgccgagcag cttaaacgca atatcaaaac
     1441 gatccgtgca cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt
     1501 gttggaggcc gcagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca
     1561 tccctattaa tccgcatgat gccgggttta cttccccggc agtgctttca tttcagcgta
     1621 caatcgccac attgctgcac atccggtaag cgataacgct ggcagcaggt tcggcgcacc
     1681 agcaggccat cgcgcagcac gacggtacgc cagagtggat tatcttcacc gttcgtgagc
     1741 gttttctcaa aaaagagggc atggcgcagc gattcaaccg tcgcctcgcc gagcagttgc
     1801 ttcatctcag tgagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg
     1861 ttgatctctc cggtcgcttc tagtgcctgc acaaccggaa ccagcgcctg gctgattaac
     1921 gtttccattc gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc
     1981 cagaaacagg cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca
     2041 tctaatgcct tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata
     2101 taccattgtg cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg
     2161 cgatagatat gatcggaata gaccgcgagt agagaactta gcgcattcgg tgatgaccat
     2221 tgcgccagcg tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctcgagcaaa
     2281 tgttcacgat gtttggcgat cgtcgcacgt acggcttgtg caagcccagc atcctgcggc
     2341 tggagatgcg ttcgccagat gatatcttca tagagcggtg cggaacgata ggccataatc
     2401 gggatagtaa tctaaatgat aatgattgct aatcctagcg ataggtttac ccgatagcaa
     2461 gggatttctc tggcttgcaa atgataaaaa ttatcatatg atattggtta tcattatcaa
     2521 tgaaagagat gaaaccatgt tgcaacgtat gctgggcagt ggctggggag tgttgctgcc
     2581 aggattgctg attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt
     2641 cattctgatg ggattagtat tgacgccggt gatgctgtat cacaaacagt tacggcatta
     2701 cgttttgcta ccatcgtgcc tggcgcttat tgctggcatc atgctgatga taatgaattt
     2761 gaatcaggga tgaaaaatca aggaagaaac aagaaaggaa gtaaagataa ttggtgcgag
     2821 gggggggact tgaaccccca cgtccgtaag gacactaaca cctgaagcta gcgcgtctac
     2881 caattccgcc accttcgcac agtcatctta ctttttttga tatcgcctcg tttggtgcga
     2941 ggggggggac ttgaaccccc acgtccgtaa gaacactaac acctgaagct agcgcgtcta
     3001 ccaattccgc caccttcgcc cagtgcgagc aatatcaacg tggtttttgg tgcgaggggg
     3061 gggacttgaa cccccacgtc cgtaaggaca ctaacacctg aagctagcgc gtctaccaat
     3121 tccgccacct tcgcatacca tcaattctta aaaagaattg ctaccacgga ggcgcattct
     3181 agtggttttc agcttttcgt caatagttaa ttatcgccgg aggtgtaatt gctggaaaaa
     3241 tgtccatcag gaaactagcg tgcaggtttg gtaggcatgc gggggcagat gccagatgcg
     3301 acgctggcgc gtcttatctg gcctacgaag ggctaacgtg caggttttgt aggtcggata
     3361 aggcgttcac gccgcatccg acacggtatt cggcgagata attaaccttt cttcgcctgg
     3421 cgggtcataa tggcgcgata caccttgaaa cgcccggttt gcgcaatcac ttcatggaag
     3481 ccaaatgtct catccagcac gtccgggtaa ggcaggaagg cgttcgctac aattcgcagc
     3541 tcgccgccgc tattaagatg acgcaccgcg ccgcgaatca gcgtttgcgc cgcatccagg
     3601 ctggtttgca tcccatcgtg gaacggcggg ttggagatga tcatatcaaa acgacctttc
     3661 acctcggaaa agacgttgct ggcaaagact tcaccttcaa caccgttggc cgcaagtgtt
     3721 gcgcggctgg cttctaccgc tggcgcagag acatcgcaca aggtgagacg aattttcggc
     3781 gaatggcgcg caaaggcaac tgaaagcaca cccgcgccac agccgacatc cagcacttta
     3841 cctttcgtgt gcggggttaa cgtcgagagc agcaactggc taccgacatc cagaccgtcg
     3901 cggctaaaca cgccaggcag cgttttgacc gtcaggccat cgacgctgta ttcgccccag
     3961 aatttatcgg catcaaatac cggctgtttt tccagacgac caaaatagag gccacagcga
     4021 cgagcgctgt cgactttatt caacggcgca taatctgcca gcatctgctc ggcgctgcgc
     4081 acgccgctgc ggttctcgcc aacgacaaaa atatctgtcc ccactggcag cagagaaagt
     4141 aaattcatca actggaactg ggcttccggt ttgttcttcg gccagtagta aatcagcgta
     4201 tcgcaatctg cgacgtcatc caccgtggcg accagactga aacgggcgtt atcccccatc
     4261 tggcggctta atacctgcca gtggtggaat tgctgggtat gagcacggct ggccgcggta
     4321 tctaaacgcg cgggcaggtc atcctgtaag tctccggcaa acagaatacg gctttgttcg
     4381 aaatcatcac tgtgacgcag caagacttca cttgccgggg taaatgcaga catggaatgc
     4441 tcctcaattg atactggcgg cgattatagc catatgttgg cgcggtatcg acgaatttgc
     4501 tatatttgcg cccctgacaa caggagcgat tcgctatgac atcccgacga gactggcagt
     4561 tacagcaact gggcattacc cagtggtcgc tgcgtcgccc tggcgcgttg cagggggaga
     4621 ttgccattgc gatcccggca cacgtccgtc tggtgatggt ggcaaacgat cttcccgccc
     4681 tgaccgatcc tttagtgagc gatgttctgc gcgcattaac cgtcagcccc gatcaagtgc
     4741 tgcaactgac gccagaaaaa atcgcgatgc tgccgcaagg cagtcgctgc aacagctggc
     4801 ggttgggtac tgacgaaccg ctatcactgg aaggcgctca ggtggcatca ccggcgctca
     4861 ccgaattacg ggcaaaccca acggcacgcg ccgcgttatg gcaacagatt tgcacatatg
     4921 aacacgattt cttccctcga aacgactgat ttaccagcgg cttaccacat tgaacaacgc
     4981 gcccacgcct ttccgtggag tgaaaaaacc tttgccagca accagggcga gcgttatctc
     5041 aactttcagt taacgcaaaa cggcaaaatg gcggcgtttg cgattacgca agtggtgctg
     5101 gatgaagcta cattgttcaa tattgcggtc gatcctgact atcagcgtca gggattggga
     5161 agggtgctgc tggaacatct gatcgacgaa ctggaaaaac gcggcgtggc gacactatgg
     5221 ctggaagtcc gtgcttcaaa cgctgccgcc attgccctgt acgaaagttt aggctttaac
     5281 gaggcgacga ttcgccgcaa ttactacccc accacggacg gtcgcgaaga cgccatcatc
     5341 atggcgttgc caatcagtat gtaagacaag gtggaataat gaagtgggac tggattttct
     5401 ttgatgccga tgaaacgctg tttacctttg actcattcac cggcctgcag cggatgtttc
     5461 ttgattacag cgtcaccttt accgctgaag attttcagga ctatcaggcc gttaacaagc
     5521 cactgtgggt ggattatcaa aacggcgcga tcacttcatt acagcttcag cacgggcggt
     5581 ttgagagctg ggccgaacgg ctgaaagttg aagcaggctt gcttaacgat gcctttatta
     5641 atgcgatggc ggaaatctgt acgccgttgc cgggcgcggt ttctctgctt aacgccattc
     5701 gtggcaacgc taaaatcggc atcatcacca acggttttag cgccttgcag caagtgcgtc
     5761 tggaacggac gggcctgcgt gattacttcg atttgctggt gatttccgaa gaagttggcg
     5821 ttgctaaacc gaataagaaa attttcgatt atgcgctgga acaggcgggc aatcctgacc
     5881 gttcacgcgt gctgatggtt ggcgacactg ccgagtccga tattctcggt ggcatcaacg
     5941 ccgggcttgc gacctgctgg ctgaatgcgc accatcgcga gcaaccagaa ggcatcgcgc
     6001 ccacctggac cgtttcttcg ttgcacgaac tggagcagct cctgtgtaaa cactgattgc
     6061 ctcccccccg ttgatgggta aaatagccgc aatttttcgt tttcaacaag cgcggcgcga
     6121 tgccgcttac tcaagaagaa agaattatga cgttgtctcc ttatttgcaa gaggtggcga
     6181 agcgccgcac ttttgccatt atttctcacc cggatgccgg taagactacc atcaccgaga
     6241 aggtgctgct gttcggacag gccattcaga ccgccggtac agtaaaaggc cgtggttcca
     6301 accagcacgc taagtcggac tggatggaga tggaaaagca gcgtgggatc tccattacta
     6361 cgtctgtgat gcagtttccg tatcacgatt gcctggttaa cctgctcgac accccggggc
     6421 acgaagactt ctcggaagat acctatcgta ccctgacggc ggtggactgc tgcctgatgg
     6481 ttatcgacgc tgcaaaaggt gttgaagatc gtacccgtaa gctgatggaa gttacccgtc
     6541 tgcgcgacac gccgatcctc acctttatga acaaacttga ccgtgatatc cgcgacccga
     6601 tggaactgct cgatgaagtt gagaacgagc tgaaaatcgg ctgcgcaccg atcacctggc
     6661 cgattggctg cggcaagctg tttaaaggcg tttaccacct ttataaagac gaaacctatc
     6721 tctatcagag cggtaaaggc cacacgattc aggaagtccg tattgttaaa gggctgaata
     6781 acccggatct cgacgctgcg gttggtgaag atctggcaca gcagctacgt gacgaactgg
     6841 aactggtgaa aggcgcgtct aacgagttcg acaaagaatt gttccttgcg ggcgaaatca
     6901 ctccggtatt cttcggtact gcgctgggta acttcggcgt tgatcatatg ctggatggcc
     6961 tggtggagtg ggcccctgcg ccgatgccgc gtcagactga tacccgtacc gtagaggcga
     7021 gcgaagacaa atttaccggc ttcgtattta aaattcaggc caacatggac ccgaaacacc
     7081 gcgaccgcgt ggcgtttatg cgcgtagtat ccggtaaata tgaaaaaggc atgaagctgc
     7141 gccaggtgcg tactgcgaaa gatgtggtta tctccgacgc gctgaccttt atggcgggcg
     7201 accgttcgca cgttgaagaa gcgtatccgg gcgatatcct cggcctgcac aaccacggca
     7261 ctattcagat cggcgacacc tttacccagg gtgagatgat gaagttcacc ggtattccga
     7321 acttcgcacc agaactgttc cgtcgtatcc gcctgaaaga tccgctgaag caaaaacagc
     7381 tgctcaaagg gctggtacag ctttccgaag agggcgcggt gcaggtgttc cgtccgatct
     7441 ccaacaacga tttgatcgtt ggtgctgttg gtgtgctgca gtttgatgtg gtggtatcgc
     7501 gcctgaagag cgaatacaac gttgaagcag tatatgaatc agtcaacgtt gccactgccc
     7561 gctgggtaga atgtgcggac gcgaagaaat tcgaagagtt caagcgtaag aacgaaagcc
     7621 aactggcgct tgatggcggc gataacctcg cttacatcgc taccagcatg gtcaacctgc
     7681 gcctggcaca ggaacgttat ccggacgttc agttccacca gacccgcgag cattaattct
     7741 tgcctccagg gcgcggcaac cgcctcgccc tgtcaatttc ccttccttat tagccgctta
     7801 cggaatgttc ttaaaacatt cacttttgct tatgttttcg ctgatatccc gagcggtttc
     7861 aaaattgtga tctatattta acaaagtaat gacatttctg acggcgttaa ataccgttca
     7921 atgcgtagat atcagtatct aaagccgtcg attgtcattc taccgatatt aataactgat
     7981 tcagaggctg taatggtcgt tattcatcac ccatcgcttt tgtgatggcg accattgact
     8041 tctgtagagg gtgaagtctc tccctattca gcaatgcaac ctcgtgttgc caggctcaaa
     8101 ttacgagcaa acatacagga ataaatcgat gactatgaca agactgaaga tttcgaaaac
     8161 tctgctggct gtaatgttga cctctgccgt cgcgaccggc tctgcctacg cggaaaacaa
     8221 cgcacagact accaatgaaa gcgcagggca aaaagtcgat agctctatga ataaagtcgg
     8281 taatttcatg gatgacagcg ccatcaccgc gaaagtgaag gcggcgctgg tggatcatga
     8341 caacatcaag agcaccgata tctctgtaaa aaccgatcaa aaagtcgtga ccctgagcgg
     8401 tttcgttgaa agccaggccc aggccgaaga ggcagtgaaa gtggcgaaag gcgttgaagg
     8461 ggtgacctct gtcagcgaca aactgcacgt tcgcgacgct aaagaaggct ctgtgaaggg
     8521 ctacgcgggt gacaccgcca ccaccagtga aatcaaagcc aaactgctgg cggacgatat
     8581 cgtcccttcc cgtcatgtga aagttgaaac caccgacggc gtggttcagc tctccggtac
     8641 cgtcgattcc caggcacaga gcgaccgtgc tgaaagtatc gccaaagcgg tagatggtgt
     8701 gaaaagcgtt aaaaatgatc tgaaaactaa gtaattcgtc gtaattcgtc ctcccgaact
     8761 ttgtcgggag gcgcgatgtg caccacacta aaaatatcgc attgagtgat tttcacgctc
     8821 atatcaagcg gtgacattaa ctatggtaaa ggagacgctt atgtttcgtt ggggcatcat
     8881 atttctggtt atcgcgttaa tcgccgccgc acttgggttt ggtggtctgg ccggtaccgc
     8941 tgcaggcgca gctaaaattg tctttgtcgt cgggattatt ctgttcctgg tgagtttgtt
     9001 catgggccga aaacgaccct agatttcaga ccattaagaa aattttatcc aaagccagtc
     9061 cagcggactg gcttttgcgg ttttagcgaa taataaattg cgttactttg tcattctttc
     9121 acaacaaaaa caggaaggca gaggtggggc agcgaatacc tgtaacgctt ggtaatattg
     9181 cgccgttgtc gctaaggccg ttccagcctg gacgaatagc tctggtgtgc gaaggcggcg
     9241 gacagcgtgg aattttcacg gctggcgtgc tggatgagtt tatgcgcgcg cagtttaatc
     9301 ctttcgatct ttatctcggc acatctgccg gggcgcagaa cctctcggcg tttatctgca
     9361 atcagcccgg ttacgcgcgc aaagtcatca tgcgctatac cacaaaacgc gaatttttcg
     9421 atccattgcg ctttgtccgt ggaggaaatc ttatcgatct cgactggctg gtggaggcca
     9481 ccgcaagcca gatgccgttg caaatggata ccgccgcgcg gttgtttgac agcggcaaat
     9541 cgttttatat gtgcgcctgt cgtcaggatg actacgcgcc taattacttt ttaccaacca
     9601 aacaaaactg gctggatgtg attcgcgcct ccagtgcgat acctggcttt tatcgtagcg
     9661 gagtgtcgct ggaaggcatt aactacttgg atggcgggat cagtgatgcg attccggtta
     9721 aagaggcggc aaggcagggc gctaaaacgt tggtcgtcat ccgcactgtg ccgtcacaaa
     9781 tgtactacac gccgcagtgg ttcaaacgca tggaacgctg gctgggtgac agtagcctgc
     9841 agccgctggt caatctggtg cagcatcatg aaaccagcta tcgtgacatt cagcaattta
     9901 ttgagaaacc accgggcaag ctgcggatat tcgaaattta tccaccgaaa ccattacata
     9961 gtatcgcgct tggcagtcgg attccggcgc tgcgtgaaga ctataaactt gggcgtttat
    10021 gcggtcgtta tttcctcgcc acggttggca agctattaac tgaaaaagcg ccgcttaccc
    10081 gccatctggt gccagtggtg acgccggaat cgatcgttat tccgcctgcg ccagtcgcca
    10141 acgatacgct ggttgccgaa gtgagcgatg ctccgcaggc gaacgacccg acatttaaca
    10201 atgaggatct ggcttgattt gccgttttat cgatacccac tgccattttg atttcccgcc
    10261 gtttagtggc gatgaagagg ccagcctgca acgcgcggca caagcgggcg taggcaagat
    10321 cattgttccg gcgacagagg cggaaaattt tgcccgcgtg caggcgttag cggaaaaata
    10381 tcaaccgctg tatgccgcat tggggttgca tccggggatg ttggaaaaac atagcgatgt
    10441 gtctcttgat cagctacagc aggcgctgga aaggcgtccg gcgaaggtgg tggcggtggg
    10501 ggagatcggt ctggatctct tcggcgacga tccgcaattt gagaggcagc agtggttact
    10561 cgacgaacag ctgaaactgg cgaaacgcta cgatctgccg gtgatccttc attcccggcg
    10621 tacacacgac aaactggcga tgcatcttaa acgtcacgat ttaccgtgca ctggcgtggt
    10681 tcacggtttt tccggcagcc tgcaacaggc cgagcggttt gtacagctgg gctacaaaat
    10741 cggcgtaggc ggtactatca cctatccacg cgccagtaaa acccgcgatg tcatcgcaaa
    10801 attaccgctg gcatcgttgt tgctggaaac cgacgcgccg gatatgccgc tcaacggctt
    10861 tcaggggcag cctaaccgac cggaacaggc tgtgcgggtg tttgacgtgc tttgcgagtt
    10921 gcgcccggaa ccagaagatg agattgccga agtgttgctt aataatacgt atgcgttgtt
    10981 tagcgttagt gggtagttta cccttcgtag gcagcatccg gcagtcggcg cataatgcct
    11041 gatgcgacgc ttaacgcgtc ttatcatgcc tacaggtttg tgccgaaccg taggccggat
    11101 aaggcgttca cgccgcatcc ggcagttggc gcacaatgcc tgatgcgacg cttgacgcgt
    11161 cttatcatgc ctacaggttt gtgccgaacc gtaggccgga taaggcgttc acgccgcatc
    11221 cggcagtcgg cgcataatgc ctgatgcgac gcttgtcgcg tcttatcatg cctacaagtc
    11281 tgtgccgaac cgtaggccgt atccggcatg tcacaaatag agcgccggaa atatcaaccg
    11341 gctcaccccg cgcaccttta acgcatcagc caacggctca acgtcttccg gcgtggcgct
    11401 cgcccagctt tgcgcctcgc catacacgcc gtgggcatga aacgcattca ggcgaaccgg
    11461 aacatcgcca agtctcttga taaacgtcgc cagctcatcg atgtgatgca aataatccac
    11521 ctggtctgga attaccagca aacgcagttc cgccagcttg ccgcgctctg caagcaaaca
    11581 gatgctgcgc ttaatctgct gattatcgcg tccggttaag tgttgatgac attcgctccc
    11641 ccacgctttg agatcgagca ttgcgccgtc acacaccggg agtaattttt cccagccggt
    11701 ttcgctcaac atgccgttac tgtccaccag gcaggtgagg tggcgcagtt gcggatcgtt
    11761 tttgatagca gtaaacagcg ccactacaaa cggcagttgg gtcgtggctt cgccgccgct
    11821 taccgttatc ccttcaataa acagcaccgc tttgcggaca tggctaagca cttcgtccac
    11881 gctcatggat tgcgccattg gcgtggcatg ttgcggacac atcttcaggc aggtatcaca
    11941 ctgctcgcag accacagcgt tccacagcac tttgccgtca acaatctgca acgcctgatg
    12001 cggacactgt ggcacgcact ctccgcaatc attgcagcgt cccatcgtcc acggattgtg
    12061 acagtttttg cagcgcagat tgcagccctg caaaaacaga gccagacgac tgcctggccc
    12121 gtcaacgcag gagaagggga taatcttact gactaaagcg catctgctgt tcatggctta
    12181 tcacgcgcgg ctggcgttcc agaatacgag tgttgcgtgc ggcttcttcg cccagccagg
    12241 tggtgttggt gcgtgaacct tcggcgcgat atttttctaa gtccgacaaa cgtaccatat
    12301 aaccggtaac gcgaaccaga tcgttaccgc tgacattggc ggtaaattca cgcattccgg
    12361 ctttaaaggc accgaggcaa agctgtacca gtgcctgcgg gttacgtttg atggtttcgt
    12421 cgagcgtcag aatgtcgctg atgccggaat aataataagc atgatgcggg gcgacagttt
    12481 gcagatgggt gatcggatct ggctcatcgc catacggcaa acgcgcgccc ggcgtggtgc
    12541 caatatcgga actgatcccc gactgtgcgt gtaacatggc gcgtttttgc catccatatt
    12601 tcacgggggt attggcgaca aactccgcca gttgcgcgct gatgcgataa cccacttcat
    12661 ttgcggcggc ttctttaccg taacgtgcgg caatcccttc tttctcacac agcaagttaa
    12721 ccgcttccgc cagcccatac atgccaaaca ttggcacaaa acgttcaggg ttaatcagcc
    12781 cttctttcac caggaagcta ttctcaaaga agtgtgattg ttgatagagg aattcacacc
    12841 gcgcatcgat gatggcgatc tgctgctggc agtagtgcgg tagagtgcgc gtaaagaagt
    12901 catccagcga ttcgctgcgc tcggcaatgg ctttcaggtt aaggcgtacc agcgtgctgc
    12961 caccacccgc cagcggcagt gagttgtaac agctcacaat cccgtagccc ccttttgtga
    13021 aaattttatc atgcaccgga ccgttggcga tgtgcggttt gctacattca cagatgttct
    13081 tcgccacttc cagcagcagg tcatcagggg tgatttcagg atcgtagata aaggtcaggt
    13141 tcggtgaaac ctgcttcaac tctgcgtctg cacgtaagat tgcacgggta atgggcgaat
    13201 cagacgggcc gatattggcg tgcataaagg cgtctggcag ggttctgtcg aggtaacgcc
    13261 agaaacgttt tattcgaaca tcgatctcgt cttgtgttag aattctaaca tacggttgca
    13321 acaacgcatc cagttgcccc aggtagaccg gcatcgatgt gaccgacggt acgtggtggt
    13381 aaagaatggt cagcagagag agtgcgtcat caagatcttt cgcgccttcc agctccagcc
    13441 attcggaacc gttcgccaga aaacgggcgt aatcgggtaa gacatagcgc ggtttgtacg
    13501 gcgcatgacc ttcaaacata tcgcagatta caccttcatc cagcgcgcgg cgggcttcgg
    13561 caggcagctg tgggtaaggc agattgtttt ctgcttccag tgccagaaaa tggcgcttct
    13621 gctccgggct aagcactggg ctggtgacaa tttgctggca acgttgttgc agtgcatttt
    13681 catgagaagt gggcatcttc ttttcctttt atgccgaagg tgatgcgcca ttgtaagaag
    13741 tttcgtgatg ttcactttga tcctgatgcg tttgccacca ctgacgcatt catttgaaag
    13801 tgaattattt gaaccagatc gcattacagt gatgcaaact tgtaagtaga tttccttaat
    13861 tgtgatgtat atcgaagtgt gttgcggagt agatgttaga atactaacaa actcgcaagg
    13921 tgaattttat tggcgacaag ccaggagaat gaaatgactg atctgaaagc aagcagcctg
    13981 cgtgcactga aattgatgga cctgaccacc ctgaatgacg acgacaccga cgagaaagta
    14041 attgctctgt gtcatcaggc caaaaccccg gtcggcaata ccgccgctat ctgtatctat
    14101 cctcgcttta tcccgattgc tcgcaaaaca ctgaaagagc agggcacccc ggaaatccgt
    14161 attgctacgg taaccaactt cccacacggt aacgatgaca tcgaaatcgc gctggcagaa
    14221 acccgtgcgg caatcgccta cggtgccgat gaagttgacg tggtgttccc gtaccgcgcg
    14281 ctgatggcgg gtaacgagca ggttggtttt gacctggtga aagcctgtaa agaggcttgc
    14341 gcggcagcga atgtactgct gaaagtgatc atcgaaaccg gcgaactgaa agacgaagcg
    14401 ctgatccgta aagcgtctga aatctccatc aaagcgggtg cggacttcat caaaacctct
    14461 accggtaaag tggctgtgaa cgcgacgccg gaaagcgcgc gcatcatgat ggaagtgatc
    14521 cgtgatatgg gcgtagaaaa aaccgttggt ttcaaaccag cgggcggcgt gcgtactgcg
    14581 gaagatgcgc agaaatatct cgccatcgca gatgaactgt tcggtgctga ctgggcagat
    14641 gcgcgtcact accgctttgg tgcttccagc ctgctggcaa gcctgttgaa agcgctgggc
    14701 cacggtgacg gtaagagcgc cagcagctac taagtaagat gctttacgcc tgatgcgctg
    14761 cgcttatctg gcctacgaga cgtatctacc cgtaggccgg ataaggcgta gacgcatccg
    14821 gcaaaagccg cctcatactc ttttcctcgg gaggttacct tgtttctcgc acaagaaatt
    14881 attcgtaaaa aacgtgatgg tcatgcactg agcgatgaag aaattcgttt ctttatcaac
    14941 ggcattcgcg acaacactat ctccgaaggg cagattgccg ccctcgcgat gaccattttc
    15001 ttccacgata tgacaatgcc tgagcgtgtc tcgctgacca tggcgatgcg agattcagga
    15061 accgttctcg actggaaaag cctgcatctg aatggcccga ttgttgataa acattccact
    15121 ggtggcgtcg gcgatgtgac ttcgctgatg ttggggccga tggtcgcagc ctgcggcggc
    15181 tatattccga tgatctctgg tcgcggcctc ggtcatactg gcggtacgct cgacaaactg
    15241 gaatccatcc ctggcttcga cattttcccg gatgacaacc gtttccgcga aattattaaa
    15301 gacgtcggcg tggcgattat cggtcagacc agctcactgg ctccggcgga taaacgtttc
    15361 tacgcgaccc gtgatattac cgcaaccgtg gactccatcc cgctgatcac cgcctctatt
    15421 ctggcgaaga aactggcgga aggtctggac gcgctggtga tggacgtgaa agtgggtagc
    15481 ggcgcgttta tgccgaccta cgaactctct gaagcccttg ccgaagcgat tgttggcgtg
    15541 gctaacggcg ctggcgtgcg caccaccgcg ctgctcaccg acatgaatca ggtactggcc
    15601 tccagtgcag gtaacgcggt tgaagttcgt gaagcggtgc agttcctgac gggtgagtat
    15661 cgtaacccgc gtctgtttga tgtcacgatg gcgctgtgcg tggagatgct tatctccggc
    15721 aaactggcga aagatgacgc cgaagcgcgc gcgaaattgc aggcagtgct ggacaacggt
    15781 aaagcggcag aagtctttgg tcgtatggta gcggcacaaa aaggcccaac cgacttcgtt
    15841 gagaactacg cgaagtatct gccgacagcg atgctgacga aagcagtcta tgctgatacc
    15901 gaaggttttg tcagtgaaat ggatacccgc gcgctgggga tggcagtggt tgcaatgggc
    15961 ggcggtcgtc gtcaggcatc tgacaccatt gattacagcg tcggctttac tgatatggcg
    16021 cgtttgggcg accaggtaga cggtcagcgt ccgctggcgg ttatccacgc gaaagacgaa
    16081 aacagctggc aggaagcggc gaaagcggtg aaagcggcaa ttaaacttgc cgataaagca
    16141 ccggaaagca caccaactgt ctatcgccgt atcagcgaat aacggtatac tgatctgatc
    16201 atttaaattt gaagcactga gtacggagaa catatgaaac gtgcatttat tatggtgctg
    16261 gactcattcg gcatcggcgc tacagaagat gcagaacgct ttggtgacgt cggggctgac
    16321 accctgggtc atatcgcaga agcttgtgcc aaaggcgaag ctgataacgg tcgtaaaggt
    16381 ccgctcaatc tgccaaatct gacccgtctg gggctggcga aagcacacga aggttctacc
    16441 ggtttcattc cggcgagaat ggacggcaac gctgaagtta tcggcgcgta cgcatgggcg
    16501 cacgaaatgt catccggtaa agataccccg tctggtcact gggaaatcgc cggtgtcccg
    16561 gttctatttg agtggggata tttctccgat cacgaaaaca gcttcccgca agagctgctg
    16621 gataaactgg ttgaacgcgc taatctgccg ggttacctcg gtaactgcca ctcttccggt
    16681 acggtcattc tggatcagct gggcgaagag cacatgaaaa ccggcaagcc gattttctat
    16741 acctccgctg actctgtgtt ccagattgcc tgccacgaag aaacctttgg cctggataaa
    16801 ctctacgaac tgtgcgaaat cgcccgtgaa gagctgacca acggcggcta caacatcggt
    16861 cgtgttatcg ctcgtccgtt tatcggtgac aaagccggta acttccagcg taccggtaac
    16921 cgtcacgacc tggctgttga gccgccagca ccgaccgtgc tgcagaaact ggttgatgaa
    16981 aaacacggtc aggtggtttc tgtcggtaaa attgcagaca tctacgccaa ctgcggtatc
    17041 accaagaaag tgaaagcaac tggcctggac gcgctgtttg acgccaccat caaagagatg
    17101 aaagaagcgg gtgataacac catcgtcttc accaacttcg ttgacttcga ctcttcctgg
    17161 ggccaccgtc gcgacgtcgc cggttatgct gcgggtctgg agctgttcga ccgccgtctg
    17221 ccggagctga tgtctctgct gcgcgatgac gacatcctga tcctcactgc tgaccacggt
    17281 tgtgatccga cctggaccgg tactgaccac acgcgtgaac acattccggt actggtttac
    17341 ggcccgaaag taaaaccggg ctcactgggt caccgtgaaa ccttcgcgga tatcggccag
    17401 actctggcaa aatattttgg tacttctgat atggaatatg gcaaagccat gttctgataa
    17461 tgggtgcggc ttgatgccct caccccggcc ctctccctca gggagagggt gaaaacatgt
    17521 tgataaggat aaaacaatgg ctaccccaca cattaatgca gaaatgggcg atttcgctga
    17581 cgtagttttg atgccaggcg acccgctgcg tgcgaagtat attgctgaaa ctttccttga
    17641 agatgcccgt gaagtgaaca acgttcgcgg tatgctgggc ttcaccggta cttacaaagg
    17701 ccgcaaaatt tccgtaatgg gtcacggtat gggtatcccg tcctgctcca tctacaccaa
    17761 agaactgatc accgatttcg gcgtgaagaa aattatccgc gtgggttcct gtggcgcagt
    17821 tctgccgcac gtaaaactgc gcgacgtcgt tatcggtatg ggtgcctgca ccgattccaa
    17881 agttaaccgc atccgtttta aagatcatga ctttgccgct atcgctgact ttgacatggt
    17941 gcgtaacgcg gtagacgcgg ctaaagcact gggcattgat gctcgcgttg gtaacctgtt
    18001 ctccgctgac ctgttctact ctccggacgg cgaaatgttc gacgtgatgg aaaaatacgg
    18061 catcctcggc gtggaaatgg aagcggctgg tatctacggc gtcgctgcag aatttggcgc
    18121 gaaagccctg accatctgta ccgtgtctga ccacatccgc actcacgagc agaccactgc
    18181 cgctgagcgt cagaccacct tcaacgacat gatcaaaatc gcactggaat ccgttctgct
    18241 gggcgataaa gagtaattgt gtttcgctga aaggcgattg tcgtgtgaag ccggagtggg
    18301 agactgctcc ggctttttag t
//
LOCUS       DAJOWB010000093        18123 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJOWB010000093 DAJOWB010000000
VERSION     DAJOWB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.093"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(110..916)
                     /gene="trpA"
                     /locus_tag="OHO85_003432"
     CDS             complement(110..916)
                     /gene="trpA"
                     /locus_tag="OHO85_003432"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCQ2672764.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(916..2109)
                     /gene="trpB"
                     /locus_tag="OHO85_003433"
     CDS             complement(916..2109)
                     /gene="trpB"
                     /locus_tag="OHO85_003433"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCQ2672765.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2121..3482)
                     /gene="trpCF"
                     /locus_tag="OHO85_003434"
     CDS             complement(2121..3482)
                     /gene="trpCF"
                     /locus_tag="OHO85_003434"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCQ2672766.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQLGIKDARLLASVFQTL
                     RAY"
     gene            complement(3483..5078)
                     /gene="trpD"
                     /locus_tag="OHO85_003435"
     CDS             complement(3483..5078)
                     /gene="trpD"
                     /locus_tag="OHO85_003435"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCQ2672767.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5078..6640)
                     /gene="trpE"
                     /locus_tag="OHO85_003436"
     CDS             complement(5078..6640)
                     /gene="trpE"
                     /locus_tag="OHO85_003436"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCQ2672768.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6661..6754)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6731..6775)
                     /gene="trpL"
                     /locus_tag="OHO85_003437"
     CDS             complement(6731..6775)
                     /gene="trpL"
                     /locus_tag="OHO85_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCQ2672769.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6913..7794
                     /gene="yciV"
                     /locus_tag="OHO85_003438"
     CDS             6913..7794
                     /gene="yciV"
                     /locus_tag="OHO85_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCQ2672770.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7791..8411
                     /locus_tag="OHO85_003439"
     CDS             7791..8411
                     /locus_tag="OHO85_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCQ2672771.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8512..9384
                     /gene="rluB"
                     /locus_tag="OHO85_003440"
     CDS             8512..9384
                     /gene="rluB"
                     /locus_tag="OHO85_003440"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCQ2672772.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(9424..10014)
                     /gene="cobO"
                     /locus_tag="OHO85_003441"
     CDS             complement(9424..10014)
                     /gene="cobO"
                     /locus_tag="OHO85_003441"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCQ2672773.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(10011..10769)
                     /locus_tag="OHO85_003442"
     CDS             complement(10011..10769)
                     /locus_tag="OHO85_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCQ2672774.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGCEAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            10989..12038
                     /gene="sohB"
                     /locus_tag="OHO85_003443"
     CDS             10989..12038
                     /gene="sohB"
                     /locus_tag="OHO85_003443"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCQ2672775.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(12074..12325)
                     /locus_tag="OHO85_003444"
     CDS             complement(12074..12325)
                     /locus_tag="OHO85_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCQ2672776.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSENYHLVD"
     gene            12705..15302
                     /gene="topA"
                     /locus_tag="OHO85_003445"
     CDS             12705..15302
                     /gene="topA"
                     /locus_tag="OHO85_003445"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCQ2672777.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            15512..16486
                     /gene="cysB"
                     /locus_tag="OHO85_003446"
     CDS             15512..16486
                     /gene="cysB"
                     /locus_tag="OHO85_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCQ2672778.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            16781..16945
                     /locus_tag="OHO85_003447"
     CDS             16781..16945
                     /locus_tag="OHO85_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCQ2672779.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            16948..17115
                     /locus_tag="OHO85_003448"
     CDS             16948..17115
                     /locus_tag="OHO85_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672780.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            17229..17324
                     /locus_tag="OHO85_003449"
     CDS             17229..17324
                     /locus_tag="OHO85_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672781.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            17488..>18123
                     /locus_tag="OHO85_003450"
     CDS             17488..>18123
                     /locus_tag="OHO85_003450"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HCQ2672782.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTD"
BASE COUNT         4439 a         4544 c         4776 g         4364 t
ORIGIN      
        1 gaccggggta agcgaaacgg taaaaagata aatattaaat gaatttagga tttttccggc
       61 ttcattaaag aaagttaaaa tgccgccagc ggaactggcg gctgtgggat taactgcgcg
      121 tcgccgcttt catcggttgt acaaaaactt tcagtgccgc cagcattttc tctggctcat
      181 taatatgttg ctcgatgatt ttaacaatgg ccgaaccaga aatcgcgccc gcagctcctg
      241 catcaatcgc tgcttttacc tgatccgggg cggaaatacc aaacccctgc aatggaggtg
      301 cagcgttgta ctctttcagc ttcgcaacca gatgattgag gggtaacgcg gcgcggtttt
      361 ctgcgccggt cacgcccgct cgtgacagca aataggtgta accacgaccg taagaggcta
      421 tctggcgcag caggtcgtca tcggcatttg gcgggcagat gaagataggt gcgacattat
      481 ggcgcaacgc ggcctggcgg aagggcgcgg actcttcaat tggcacatcg gcaaccagca
      541 ccgaatcgac gccgactttc tcgcactggg cataaaactc atcaatgcct ttgttaaaca
      601 ccagattggc atacatcaac aggccaatgg gaatggtcgg gtgtttctgg cgaatcagtg
      661 ccagcatttc aaaacattgt gccggagtca cacctgccgc aaaggcgcgc agagtggcgt
      721 tttgaatggt cgggccatcc gccagtgggt cggagaaggg gatacctaac tccagcgcgt
      781 cagcaccggc ttcaattagc gtatcgataa ttttcaatga ctgctcaatg cccggatcac
      841 cgagcgtgac gaaaggaacg aatgcgcctt ctttgcgctc cttcaactgg gcaaacagag
      901 attcgtagcg ttccatcaga tttcccctcg tgctttcaaa atatcgtgaa cggtgaagat
      961 gtctttatcg ccgcgaccgg aaaggttaac caccagtagc tgctctttat ccgggttttc
     1021 gcgcatcatt ttcaacgcat gggccagggc gtgggaggat tccagcgccg ggatgatccc
     1081 ttcgtgcagg cacagcgttt tgaaggcttc aagggcttca tcatcggtaa tagacacgta
     1141 atcagcgcgt ccagtgctgt taagatacgc gtgttgtggg ccgacagacg ggaaatccag
     1201 tccggcggag atggagtaag attcttcaat ctgcccgtct tcggtttgca tcatcggcgc
     1261 tttcataccg aaatagatgc ccacgcgacc atgttttaac ggtgcgccgt gctcgccagt
     1321 ttcgataccg tgaccacctg gctccacacc aatcaggccg acgttggttt cattgatgaa
     1381 atcagcaaac atgccgatgg cattcgaacc gccgccaaca caggcgataa cggcatccgg
     1441 caggcgacct tctctttcca gaatctgcgc tttggtttct tcgccaatca tccgctgaaa
     1501 ctcacgcaca atggtcggat aaggatgcgg gccagctgcg gtgcccagca tatagtgcgc
     1561 ggtttcgtaa ctaccggacc agtcgcgcag cgcctcgtta caggcatctt tcagcgtcgc
     1621 ggaaccgcta tgcaccggga tcacttccgc acccattaag cgcatacgaa aaacgttagg
     1681 cgactggcgt tctacgtctt tggcacccat ataaatacgg catttcaggc cgagcagggc
     1741 gctggcaagg gccgacgcca cgccatgctg accggcaccg gtttcggcga tgatttcggt
     1801 tttacccatc cgcttcgcca gtaaagcctg accgagcacc tggttagttt tatgcgcgcc
     1861 gccgtgcagc aaatcttcac gcttgagata cagagtggtg ttcgtcccgg ctgtaatgtt
     1921 ctggcatttg gtcagcgcgg ttggacgccc ggcatagttt ttcagcaggt cgttgaactg
     1981 agcctgaaat tcaggatctt tttgcgcact gacaaaagct tcttccagct ggcgcagagc
     2041 aggcatcagg atttgtggca cgtacatgcc gccaaactca ccaaaatagg ggttaagtaa
     2101 tgttgtcatt gttcctttcc ttaatatgcg cgcagcgtct ggaaaaccga ggccaaaaga
     2161 cgtgcgtctt tgatgcccag ttgcgactct acagcagaat taaaatcaag tccggcgcag
     2221 ccggtttgtg ccgcttccac gcagttatct gcgcctaagc cccccgccag cagaacgttg
     2281 ccaagcgatt gaccatttaa tagtgaccag tcgaaacgtt gcccgcttcc accctggccg
     2341 ttgtctaaaa catatttatc aacgtgctga agctcgcggg cgggcagggt ttcaccgacg
     2401 cttaatgctt tccagatggc aacgtgtgct ggcagagctt cacgcagcgt atcgatatac
     2461 agctgatctt cattaccatg cagttgcact gccgccagcg ataacacctt agctttgtcc
     2521 accacatcgg caatatcgtg attgcggaac acgccaacat actgcaacgg tgccgcagcc
     2581 atcacttcct gcgcctgttc aacgttgacg caacgcggtg atgtcgcaac aaaaatcaac
     2641 ccaccgtaaa tcgcgcccgc gtcataagct gctttagcat cttgcccacg cgtcaggcca
     2701 catactttat tctcacccag caacacccgg cgcacggcgg cgtgcaaatc gtcatgggcc
     2761 atcaacgccg aaccaatcag aaaaccgtta gcgaagtggc ttaactcgcg cacctgagcg
     2821 taagtattga tgccggattc gctgattacc gttacgttgt gccccagttt cggcgcaagc
     2881 tcgcgggtac ggttgagatc aatcgacaaa tcacgcagat cgcggttgtt gatgccaacg
     2941 acctttgccc ccaatgcaat ggcacgctcc agttcctctt cattactgac ttcggtcagc
     3001 acacccatct ccagactgtg ggcgacggcg gcaagctggc gatattgttc gtcatccagt
     3061 actgaaagca ttaataagca ggcatcagcc tggtaatagc gcgccagata gatctggtaa
     3121 ggatcgataa tgaagtcttt acataaaatc ggctgcgggg cgatttggct gacgatgggg
     3181 aggaaatcaa agctcccctg aaaatatttc tcatcagtca gcaccgaaat tgccgaagcg
     3241 taatgtttat aaatggcggc aatgcgtgct ggatcgaaat catcacggat cacgcctttt
     3301 gacggcgacg ctttcttgca ctccagaata aacgccgtgc gcgcaccctg tagcgcatca
     3361 taaaaatgtc gcgtgctcgg ctgaacctca ttctgaaaac tggccagcgg ttgctgctgt
     3421 ttgcgggttt ctacccaaat cgccttgtct gcgacgattt tcgctaaaac ggtttgcatc
     3481 atttaccctc gtgccgccag cgcggtaact ctgtcgtaag cggaaccact gcgcagtacc
     3541 tcaagaacgg tttgcgcatt ggcttgcaga tcttcatggc catgcaggcg cattaacatg
     3601 gcgacgttcg cagcgacggc tgcttcatgg gcggcgtcgc ctttaccttg taacaaacgt
     3661 gttaaaatgt cacggttttc ttccggtgtt ccgcctgcca gttgctcctg gtggtagggt
     3721 gtcaggccaa aatcttcagc ggtcaattga tagctcttaa tttcgccgtc atgcagctcg
     3781 gcaacgattg tcggcgcgtg taatgaaact tcatccatcc cgccgctgtg caccaccgcc
     3841 gcgcgttgat accccagcac gcgcaaggtt tcggcaatcg gcagcaccag ttccggacta
     3901 taaacaccaa ttaacgccag cggcggatgc gccgggttaa tcaatggccc cagcacattg
     3961 aacagggtgc gggtttttag ttgctggcga accggcattg catggcgaaa tccggtgtga
     4021 tatttcggtg caaagaggaa acatacgcct aactcatcca gcgcctggcg cgatttatcg
     4081 gcgttcatat caagattaat accgaacgcc gccagcagat cggacgaacc agatttactg
     4141 gagacgctac ggttgccgtg tttcgccact ttcagcccac aggccgcggc gacaaacgca
     4201 ctggcggtag aaatattgat actgttgctg ccgtcaccgc cagtaccgac gatatcagca
     4261 aacagataat ccgggcgcgg gaacggcgct gcgttttcca gtagcgcggt tgctgccccg
     4321 gcgatctcgt tcgggtgctc accgcgaatt ttcatgctca ccagcgccgc cgccagttgt
     4381 tccggcttca gctcgccacg caccaccgct gaaaacagct ggtggctttc ttgttggcta
     4441 agcgtctgcg cctgatacag tttttccaga atcggttgca gcgtgttggc tggctctagt
     4501 ttctgctgcg cccaggccag cgtttgttcc agcaggcgag cgccctgggt ggtgagaatg
     4561 gattccggat ggaactggaa tccacaaacg cgatccgcat cgtgacgtac tgccatcacc
     4621 atgccattaa aatgggcgtt gatggttaaa ccggccggaa tgttactgcc aaccagcgag
     4681 tgataacgcg ccaccggcag cgggtttgtt aatccggcaa acatcgcctg accgtcatgt
     4741 tcaatgctcg acgctttacc gtgaagaatt tcgcccgcct gaccgacata gcccccgtaa
     4801 gcttcgacaa ttgcctgatg tccgaggcaa atgccaataa tgggcagctt gccacgcaag
     4861 cgggtgagga gttccggcat acaaccggct tcgctcggca caccggggcc aggagaaagc
     4921 atcagtaccg gattgctcat ggtcgccagg cgttcaatta aggtctgcgc cggaatatgg
     4981 ttgcggtaaa tcaccacgtt atgcccattg ctgcgcaact gatctgccag gttgtacgta
     5041 aaagagtcga tattatcgag cagcagtatg tcagccatca gaaagtctcc tgtgcatgat
     5101 gcgcggtggc aatagcgcgc agtacagcgc gggctttatt acgggtttcg tcggcttccg
     5161 actgcggaat agaatcaagg actacgccag caccggcttg cacggtggcg ataccgtttt
     5221 ccaccagcgc cgagcggatc acaatgcagg tgtcgagatc gccgtgcgcg gtgaaataac
     5281 ctaccgcgcc gccgtagctg ccgcggcgac gaccttccgc ctcggcaatt aactgcatgg
     5341 cgcgcacttt cggcgcaccg cttaacgtcc ccatattcat acaggcgcgg taagcgtgta
     5401 gggcgtcgag atcgtggcgc agctcaccga ccacgcggga gaccaggtgc atcacgtaag
     5461 agtaacggtc aactttggtg aggtcggcga cgtagcggct gccgggggtg caaatgcgtg
     5521 ccagatcatt acgggcaaga tccaccagca tcagatgttc agaaagctct ttatgatcgg
     5581 tacgcatttc cagttcgatg cggctgtcga ggtctctgtc cagcgagcca tcggcacgac
     5641 gaccgcgtgg acgtgttccg gcaatcgggt agatctcaat ctggcggctg gtggcgtcat
     5701 acttgagcga actttccggc gacgcgccaa acagggtgaa atcattatcc tgcataaaaa
     5761 acatgtacgg gctgggatta ctctttttca gcacgtaata ggccgccagc ggtgacgggc
     5821 agggcagaga gaaacggcga gatggcacca cctggaaaat ttctccagcg cgaatcgctt
     5881 tttgcaacaa acgcactacg ccaccgaact cttcatcgct ctgattacat tcacaacgca
     5941 tatgcggcac ggaaaccacc ggcagcggcg gcgcggcttc ggtcagttgc tgacgtagtt
     6001 cgttcaggcg agcagtgaga cgttgttttt cttcttcatt cggagcaaac aggctggcct
     6061 gaatacgggt gctttttttc tgatggtcaa tcaccatcag agtttcagcg agataaaaac
     6121 agaaatcagg gcagttattt tccgctgaca gttgcggtaa atcttcaaat cccgccacaa
     6181 ggtcataaga gaacaggccg ccgaagaaca ttgcttctcg ttcttccttc ggtacattca
     6241 acagattctg caataaacgg aaagcgtcaa aaaccgaaag ggagcataag cgagcgtctt
     6301 catccagcag tggactgaca ggggggaagc gcagcacacg gcagtttggt aattgttcat
     6361 tttccacacc cgcaggcagg gcgttatcca gtagtgtcag cagggcttcg ccgttgccgg
     6421 aaagtgcctg gattgtgaca gtgtcaccta aagctgtaat gcgcagcgca ctgtctacca
     6481 gcagcaggct ttttaaatca tctttgctgt cgatatctgc ggattccagc agcagcgttg
     6541 ccggacgatc cccacacaac tggtgaaaaa gcgcggtggg attgtcgcga taagcgcctt
     6601 cgcaggttag cagttcgaga gtcggttttt gtgtttgcat tgttattctc taattttgtt
     6661 caaaaaaagc ccgctcatta ggcgggctgg gtatctgatt gctttacgca tggtgaatac
     6721 actgcccgtt tcaggaagtg cgccaccaac ctttcagtac gaaaattgct ttcattgtcg
     6781 ataccctttt tacgtgaact tgcgtactag ttaactagtt cgatgattaa ttgtcaacag
     6841 ctcatttcag aatatttgcc agaaccgtta tgatgtcggc gcaaaaaaca ttatccagaa
     6901 cgggagtgcg ccttgagcga cacgaattat gcagtgattt acgacctgca cagccatacc
     6961 acagcttccg atggctgcct gacgccagaa gcattggtgc accgtgcagt cgagatgcgc
     7021 gtcggcaccc tggcgatcac cgaccatgac accacagctg caatcgcgcc agcaagagag
     7081 gaaatttcac gttcgggact ggcgttgaat cttattcccg gcgtggaaat ttccacggtc
     7141 tgggaaaatc atgaaattca tattgtcggg ctgaatattg atattactca tccgctaatg
     7201 tgtgagttcc tcgcgcagca gacagaacgg cgcaatcagc gggcgcagct gattgccgaa
     7261 agacttgaaa aagcgcaaat acctggggcg ctggaaggcg cacaacgact ggcgcagggc
     7321 ggcgcagtga cgcgcggtca ttttgcgcgt ttccttgttg agtgcggcaa agccagttca
     7381 atggcggatg tctttaaaaa gtatctggcg cgcgggaaaa ccggatacgt tccgccacag
     7441 tggtgtacaa tagaacaagc tattgatgtc attcatcatt ctggcggtaa ggcggtgcta
     7501 gctcatcctg ggcggtacaa tcttagtgct aaatggctga aaagattggt tgcgcatttt
     7561 gccgaacacc acggtgacgc gatggaagtc gcgcagtgcc agcaatcgcc caatgaacgt
     7621 acccagctgg cggcccttgc gcgtcagcat catttatggg catcacaagg atctgatttt
     7681 catcagccat gcccgtggat cgaactgggt cgtaaactct ggctgcccgc aggcgttgaa
     7741 ggcgtctggc agctatggga acagccgcag aacaccacag agagggaatt atgagccagt
     7801 ttttttatat tcatcctgat aacccacagc aacgtctgat caaccaggcg gtggagatcg
     7861 tgcgtaaagg cggggtgatt gtttatccaa ctgattccgg ctatgcgctc ggctgtaaaa
     7921 ttgaagataa aaacgcgatg gagcgtattt gtcgtattcg ccagctgccg gacggtcaca
     7981 actttaccct gatgtgtcgc gatctttctg aactctcgac ctattcattt gttgataacg
     8041 ttgcgtttcg tttaatgaaa aacaacacgc cgggcaacta taccttcatc ttgaagggga
     8101 cgaaggaagt gccacgccgt ctgttgcagg aaaaacgcaa aaccatcggt atgcgtgtgc
     8161 cttctaaccc gattgcgcag gcgttgcttg aagcactggg cgaaccgatg ctttccactt
     8221 cgctaatgct gccaggcagc gaatttaccg aatccgatcc cgaagaaatt aaagaccgtc
     8281 tggaaaaaca ggtggatttg attatccacg gcggctatct cggacagaaa ccgacaacgg
     8341 ttattgatct taccgatgat acgccggtcg tggtgcgtga aggcgtaggt gatgtgaagc
     8401 ctttcttata agtgcgtaac tctgtatact acgcggcctt aaaatggcgc tatacggcgc
     8461 cattgattct cctgttcgac gcctgtgaag gcgacaccaa aaggaagctc tatgagcgaa
     8521 aaattacaga aagtgctggc ccgcgctggc cacggctccc gccgtgaaat cgaatctatt
     8581 attgaagccg gtcgtgtgag tgttgatggc aaaattgcta aactcggcga tcgcgttgaa
     8641 gttacccctg ggctgaaaat tcgtatcgat ggtcatctga tttcggtacg tgagtccgct
     8701 gaacaaattt gtcgcgttct ggcctattac aaaccggaag gtgagttgtg tacccgtaac
     8761 gatccggaag ggcgtccgac ggtgtttgac cgtctgccaa agctgcgtgg cgcacgctgg
     8821 attgccgtag gtcgtctgga cgttaacacc tgtggtctgc tgctgttcac caccgatggt
     8881 gaactggcaa accgtttaat gcatccaagc cgtgaagttg aacgtgaata cgccgtgcgt
     8941 gtatttggtc aggttgacga cgcgaaactg cgtgatttga gtcgtggcgt gcaactggaa
     9001 gatggtccgg cagcttttaa aaccatcaag ttcagcggcg gcgaagggat caaccagtgg
     9061 tacaacgtga ctctgaccga aggccgtaac cgtgaagttc gtcgtctgtg ggaagcggtt
     9121 ggtgtgcagg tgagccgcct gatccgtgtt cgttacggtg atatcccact gccaaaaggt
     9181 ctgccacgcg gtggctggac ggagctggac ctcgcccaga ctaactatct gcgcgaactg
     9241 gtggagcttc cgccagaaac cagctctaaa gtcgctgtag aaaaagaccg tcgtcgcatg
     9301 aaggctaatc agattcgtcg tgcggtgaaa cgtcacagtc aggtgagcgg cggtcgtcgt
     9361 tctggcgggc gtaataacgg ttaatcaaag tatgccggac gtcatatccg gcatttttac
     9421 agattaataa tcaatcccta tttgcgcctt aacaccggca tcaaacgcat gtttgatggg
     9481 gcgcagctca cttaccgtat ccgccagttc aagaatatcc cgatgacagc ctcgacccgt
     9541 gataatcacc gtctgttgat gtggacgttc atttaacgcc tgcaccactt cttccagtgg
     9601 caaatagtca tacgccacca tatacgtcag ttcatcaagc aaaaccatat ccagtgagga
     9661 atcagcaagc atccgctttg catgttgcca gacttcgcgg caggcggcgg tatcagactc
     9721 gcggttttgt gtatcccagg taaagcccgt tgccatcacc tgaaactcaa cgccatgtgg
     9781 ctccagcaga ttgcgttcgc cattaggcca ggtgccttta ataaactgca cgacgcctac
     9841 tttttttccg tgaccaactg cgcgtgttgc cgtaccaaat gccgcggtgg ttttgccttt
     9901 tccattgccg gtaaagacga tgataatacc gcgttcatcc tgggcctggg ccacacgagc
     9961 atctactttt tctttcactc gctgctgacg ctgttggtag cgttcatcac tcattgggaa
    10021 attcctggtt tacggcccgg ttgggcgtca aaggtcatgc cggttttacg gcggctgtca
    10081 tcgcccatca gccagaggta gagcggcatg atatcagcgg gtgttttaag tttctgtgga
    10141 tcttcggtcg ggaaggcgct ggcacgcatt gcggtgcgcg taccgcctgg gttaatgcag
    10201 ttgacgcgca ggcgctgctg atattcatcg gccagtacct gcatcatccc ttcggtggca
    10261 aatttcgacg ctgcatatgc gccccagttg gctcgtccct gacgtccaac gcttgatgaa
    10321 gtaaagacca gtgaaccggc gtccgatttg agtaataaag gaagcagtgc ctgggtgagc
    10381 ataaaggtgg cattaacgtt gacctgtatg acgtcctgcc agacctgtgg attttgttcg
    10441 ctcattgggc aaacatcgcc gagcaatccg gcattatgca aaacaccatc cagacgcgga
    10501 taattaacgg taatgcgctg tgccagttgt tggcaatctt cggacgtgca ggtcagcaaa
    10561 tcgagaataa accactgtgg ctgacgtcca gtttcttcgt ttatgtggct ggctacctga
    10621 cgtaattttt cttcattacg gcccaacaga atcactgtcg caccatagcg tgcatacgtc
    10681 atcgcggctt cacagccaat cccatcgctg gctcccgtca ccaggataat gcgatcattg
    10741 agtaaatctt gttttggctg gtaatgcatg gctactcctc aacgacgttg tctgtcgtgc
    10801 tgccagtgta ctttatgact ttggggcttt atgcctgaaa cagaagggca tttcaatcag
    10861 cctggggcga taacaccgca aaatggatac tttgtcatac tttcgctgca ataacatctc
    10921 tgcgagacgg cttaacatgc ctgttgtaaa ctgtgagcca aagcgttgtt taaccaaggt
    10981 ggggactcgt ggaattgttg tctgaatatg gtttgttttt ggcgaaaatc gttaccgttg
    11041 tgctagcgat tgcggcgatt gccgccatta ttgtcaatgt tgctcaacgt aataaacgcc
    11101 agcgtggcga gttacgggtc aacaatctca gcgaacagta taaggagatg aaagaagaac
    11161 tggccgcggc gctgatggac tcacatcagc aaaaacagtg gcacaaagcg cagaagaaaa
    11221 agcacaagca agaagcgaaa gcagcaaaag cgaaagccaa actgggcgag gtggcaactg
    11281 acagtaaacc tcgcgtctgg gtgctggatt ttaaaggcag catggacgcc catgaagtga
    11341 actcgctacg tgaagagata acggcggtac tcgcagcatt caaaccgcag gatcaggttg
    11401 tgctccgtct ggaaagccct ggtggcatgg tgcatggtta cgggttggcg gcttcgcagc
    11461 tgcagcgtct gcgtgataaa aacattcctt taactgttac ggtagacaaa gtcgctgcca
    11521 gcggcggtta catgatggcc tgtgtggcgg acaaaattgt ttccgcaccg tttgctattg
    11581 tgggttccat tggggtggtg gcgcaaatgc ccaactttaa ccgcttcctg aaaagcaaag
    11641 atattgatat cgaactgcac actgccgggc agtataagcg tactctgacg ttgctgggtg
    11701 aaaataccga agaagggcgg gagaaattcc gcgaagagtt gaacgaaacg catcagttgt
    11761 ttaaagattt tgtgaagcgt atgcgtccgt ctctggatat tgaacaggtg gcaacgggtg
    11821 aacactggta cggacaacag gcggtagaga aaggcctggt tgatgaaatc aacaccagtg
    11881 atgaagttat tcttagcctg atggaaggcc gtgaagtggt taatgtacgc tatatgcagc
    11941 gtaaacgact cattgaccga ttcaccggca gcgcggcaga gagcgccgat cgattgttgc
    12001 tacgctggtg gcagcggggt caaaagccat tgatgtaaaa gacaaacgcg aggctaagac
    12061 ctcgcgtttt gctttaatca accagatgat agttttctga aagcacatgg gccaggtgtt
    12121 tgaacatatt aaacaccgcg gtgcttttgg ctgttggcaa tccttgttca tctaaaaagt
    12181 agtcgccggt aaataccagc acgccattac gctgcgtgac gccggtggct tcaatccctg
    12241 cgagcgtatc ctcatgctca cgaatgattt tgttggcctc tttcaacagc gtttcgcggt
    12301 cgatgggttg tgtctctttg ttcattattt actccttaaa caaggacatt agtctacgcc
    12361 aggcatggct tgcagacaaa tataccacgc tggtggcaag agcgccttac tggtaacttt
    12421 ggattttgca tgctaataaa gttgcgtatc ggattttatc aggtacagtg tgacgctttc
    12481 gtcaatctgg caatagattt gcttgacatt cgaccaaaat tccgtcgtgc tatagcgcct
    12541 gtaggccaag acctgttaac tcagtcacct gaattttcgt gaacagagtc acgacaaggg
    12601 gttgatatcc gcagagaacg agtccatatc ggtaactcgt tgccagtgga aggtttatca
    12661 acgtgcgacg cattcctgga agaatcaaat taggtaaggt gaatatgggt aaagctcttg
    12721 tcatcgttga gtccccggca aaagccaaaa cgatcaacaa gtatctgggt agtgactacg
    12781 tggtgaaatc cagcgtcggt cacatccgcg atttgccgac cagtggctca gctgccaaaa
    12841 agagtgccga ctctacctcc accaagacgg ctaaaaagcc taaaaaggat gaacgtggcg
    12901 ctctcgtcaa ccgtatgggg gttgacccgt ggcacaattg ggaggcgcac tatgaagtgt
    12961 tgcctggtaa agagaaggtc gtctctgaac tgaaacaact ggctgaaaaa gccgaccaca
    13021 tctatctcgc aaccgacctt gaccgcgaag gggaagccat tgcatggcac ctgcgggaag
    13081 tgattggtgg tgatgatgcg cgctatagcc gagtggtgtt taacgaaatt actaaaaacg
    13141 cgatccgcca ggcatttaac aaaccgggtg agctgaatat tgatcgtgtt aatgcccagc
    13201 aggcgcgtcg ctttatggac cgcgtggttg ggtatatggt ttcgccgctg ctatggaaaa
    13261 agatcgctcg tggtctgtct gccggtcgtg tgcagtcggt ggcggttcgc ctggtggtcg
    13321 agcgtgagcg tgaaattaaa gcgttcgtgc cggaagagtt ctgggaagtc gatgccagca
    13381 cgaccacgcc atctggtgaa gcgttggcgt tgcaggtgac tcatcagaac gacaaaccgt
    13441 tccgtccggt caacaaagaa caaactcagg ctgcggtaag tctgctggaa aaagcgcgct
    13501 acagcgtgct ggaacgtgaa gacaaaccga caaccagtaa acctggcgct ccttttatta
    13561 cctctacgct gcaacaagct gccagcaccc gtcttggatt tggcgtgaaa aaaaccatga
    13621 tgatggcgca gcgtttgtat gaagcaggct atatcactta catgcgtacc gactccacta
    13681 acctgagtca ggacgcggta aatatggttc gcggttatat cagcgataat tttggtaaga
    13741 aatatctgcc ggaaagtccg aatcagtacg ccagcaaaga aaactcacag gaagcgcatg
    13801 aagcgattcg tccttctgac gtcaatgtga tggcggaatc gctgaaggat atggaagcag
    13861 atgcgcagaa actgtaccag ttaatctggc gtcagttcgt agcctgccag atgaccccag
    13921 cgaaatatga ctccacgacg ctgaccgttg gtgcgggcga tttccgcctg aaagcacgcg
    13981 gtcgtatttt gcgttttgat ggctggacaa aagtgatgcc tgcgttgcgt aaaggcgatg
    14041 aagatcgcat cttaccagca gttaataaag gcgatgctct gacgctcgtt gaacttacac
    14101 cagcccagca ctttaccaag ccgccagccc gtttcagtga agcatcgctg gttaaagagc
    14161 tggaaaaacg tggtatcggt cgtccgtcta cctatgcgtc gatcatttcg accattcagg
    14221 atcgtggcta cgtgcgagta gaaaatcgtc gtttctatgc ggaaaaaatg ggcgaaatcg
    14281 tcaccgatcg ccttgaagaa aatttccgcg agttaatgaa ctacgacttt accgcgcaga
    14341 tggaaaacag cctcgaccag gtggcaaatc acgaagcaga gtggaaagct gtactggatc
    14401 acttcttctc ggatttcacc cagcagttag ataaagctga aaaagatccg gaagagggtg
    14461 gtatgcgccc gaaccagatg gttctgacca gcattgactg cccgacttgt ggtcgcaaaa
    14521 tggggattcg cacagcgagc accggggtat tccttggctg ttctggctat gcgctgccgc
    14581 cgaaagagcg ttgcaaaacc accattaacc tggtgccgga aaacgaagtg ctgaacgtgc
    14641 tggaaggcga agatgctgaa accaacgcgc tgcgcgcaaa acgtcgttgc ccgaaatgcg
    14701 gcacggcgat ggacagctat ctcatcgatc cgaaacgtaa gttgcatgtc tgtggtaata
    14761 acccaacctg cgacggttac gagatcgaag agggcgaatt ccgcattaaa ggttatgacg
    14821 gcccgatcgt tgagtgtgaa aaatgtggct ctgaaatgca cctgaaaatg gggcgtttcg
    14881 gtaaatacat ggcctgcacc aacgaagagt gtaaaaacac acgtaagatt ttacgtaacg
    14941 gcgaagtggc accaccgaaa gaagatccgg tgccattacc tgagctgccg tgcgaaaaat
    15001 cagatgctta tttcgtgctg cgtgacggtg ctgccggtgt gttcctggct gccaacactt
    15061 tcccgaaatc gcgtgaaacg cgtgcgccac tggtggaaga gctttatcgc ttccgcgacc
    15121 gtctgccgga aaaactgcgt tatctggccg atgcgccaca gcaggatccg gaaggtaata
    15181 agaccatggt tcgctttagc cgtaaaacca aacagcaata tgtctcttcg gaaaaagacg
    15241 gaaaggcgac tggctggtca gcattttatg ttgatggcaa atgggttgaa ggaaaaaaat
    15301 aacctttaat tctgtcaggt ttttataaac aaagggtcgc gaaagcggcc cttttttatt
    15361 gcatattatt ttttcttcac acctatacac taaggctata aatgatatag tggttatagt
    15421 tagcaccttt tttattatta aatcgtatta gtcacccgcc aggtgtgacg aaaaaacgat
    15481 gttctgatgg cgtctaagtg gatggtttaa catgaaatta caacaacttc gctatattgt
    15541 tgaggtggtc aatcataacc tgaatgtctc atcaacagcg gaaggacttt acacatcaca
    15601 acccgggatc agtaaacaag tcagaatgct ggaagacgag ctaggcattc aaattttttc
    15661 ccgaagcggc aagcacctga cgcaggtaac gccagcagga caagaaataa ttcgtatcgc
    15721 tcgcgaagtc ctgtcgaaag tcgatgccat aaaatcggtt gccggtgagc acacctggcc
    15781 ggataaaggc tcgctgtata tcgccaccac gcatacccag gcacgctacg cgttaccaaa
    15841 cgttatcaaa ggctttattg agcgttatcc ccgcgtttct ttgcatatgc accagggctc
    15901 gccgacacaa attgctgatg ccgtctctaa aggcaatgct gatttcgcta tcgccacaga
    15961 agcgctgcat ctgtatgaag atttagtgat gttaccgtgc taccactgga atcgggctat
    16021 tgtagtcact ccggatcacc cgctggcagg caaaaaagcc attaccattg aagaactggc
    16081 gcaatatccg ttggtgacat ataccttcgg ctttaccgga cgttcagaac tggatactgc
    16141 ctttaaccgc gcagggttaa cgccgcgtat cgtcttcacg gcaacggatg ctgacgtcat
    16201 taaaacttac gtccggttag ggctgggggt aggggtcatt gccagcatgg cggtggatcc
    16261 ggtcgccgat cccgaccttg tgcgtgttga tgctcacgat atcttcagcc acagtacaac
    16321 caaaattggt tttcgccgta gtactttctt gcgcagttat atgtatgatt tcattcagcg
    16381 ttttgcaccg catttaacgc gtgatgtcgt tgatgcggct gtcgcattgc gctctaatga
    16441 agaaattgag gtcatgttta aagatataaa actgccggaa aaataatttc ttgcgttatt
    16501 ttcggcacct tttatgtagc gaaggtgccg gaatatattc tcttttgtta cttatttcta
    16561 taccgtgcac aatattttta tccctcatat ttatagggta aattacctgt aaagtagtgt
    16621 ctaattaccc gcctcgcaaa ctccagtttt caaatatcta tttcaggtca aaagattgaa
    16681 ttattcactg gagacgattc gtaaattcgc tggatttttg actaaagttt ctttaggatt
    16741 tgtctcatcg tatgattaat tacaccaatc gttcaacgtc ttgctgataa gtgatggcga
    16801 ttatatgagg ttagcaatgc cttctggaaa tcaggaaccc cgccgcgatc ctgaattaaa
    16861 acgtaaagcc tggctggcgg tttttcttgg ttctgcactt ttctgggtgg ttgtcgcact
    16921 gctgatttgg aaagtgtggg gataactatg gtcggtcagg agcaactgga gtcgtcacca
    16981 ttatgccagc atagtgacaa tgaaacagaa acgaaacggg aatgttccgt cgttattcca
    17041 gacgactggc aactaacatc gcagcagcaa gcctttatag aactgtttgc tgaagatgat
    17101 cagccgaaac aataattatc atcattctta ttacccattt ttaatgaatt aaagggcttt
    17161 taatacaccg cagcaataac agcttgagtt atctcaacac aaaataataa ccgttaaggg
    17221 tgtagactat gatcaacaca aatatgaaat attggtcctg gatgggcgcg ttttctctgt
    17281 cgatgctctt ctgggccgaa ctcctctgga tcattactca ctgatccttg accccgctgc
    17341 ggcggggttg tcatttgctt tgccacaagg tttctcctct tttatcaatt tgggttgtta
    17401 tcaaatcgtt acgcgatgtt tgtgttatct ttaatattca ccctgaagag aatcagggct
    17461 tcgcaaccct gtcattaagg aggagctatg tcgtcaaccc tacgagaagc cagtaaggac
    17521 acgttgcagg ccaaagataa aacttaccac tactacagcc tgccgcttgc tgctaaatca
    17581 ctgggcgata tcacccgtct gcccaagtca ctcaaagttt tgctcgaaaa cctgctgcgc
    17641 tggcaggatg gtaactcggt taccgaagag gatatccacg cgctggcagg atggctgaaa
    17701 aatgcccatg ctgaccgtga aattgcctac cgcccggcaa gggtgctgat gcaggacttt
    17761 accggcgtac ctgccgttgt tgatctggcg gcaatgcgcg aagcggttaa acgcctcggc
    17821 ggcgatactg caaaggttaa cccgctctca ccggtcgacc tggtcattga ccactcggtg
    17881 accgtcgatc gttttggtga tgatgaggca tttgaagaaa acgtacgcct ggaaatggag
    17941 cgcaaccacg aacgttatgt gttcctgaaa tggggaaagc aagcgttcag tcggtttagc
    18001 gtcgtgccgc caggcacagg catttgccat caggttaacc tcgaatatct cggcaaagca
    18061 gtgtggagtg aattgcagga cggtgaatgg attgcttatc cggatacact cgttggtact
    18121 gac
//
LOCUS       DAJOWB010000094        18057 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJOWB010000094 DAJOWB010000000
VERSION     DAJOWB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18057
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.094"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            30..737
                     /locus_tag="OHO85_003451"
     CDS             30..737
                     /locus_tag="OHO85_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCQ2672783.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            734..2185
                     /gene="djlC"
                     /locus_tag="OHO85_003452"
     CDS             734..2185
                     /gene="djlC"
                     /locus_tag="OHO85_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCQ2672784.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(2245..3915)
                     /gene="hscC"
                     /locus_tag="OHO85_003453"
     CDS             complement(2245..3915)
                     /gene="hscC"
                     /locus_tag="OHO85_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCQ2672785.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDEARECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGISGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(3999..4934)
                     /gene="rihA"
                     /locus_tag="OHO85_003454"
     CDS             complement(3999..4934)
                     /gene="rihA"
                     /locus_tag="OHO85_003454"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCQ2672786.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(5052..5777)
                     /gene="gltL"
                     /locus_tag="OHO85_003455"
     CDS             complement(5052..5777)
                     /gene="gltL"
                     /locus_tag="OHO85_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCQ2672787.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5777..6451)
                     /gene="gltK"
                     /locus_tag="OHO85_003456"
     CDS             complement(5777..6451)
                     /gene="gltK"
                     /locus_tag="OHO85_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCQ2672788.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6451..7191)
                     /gene="gltJ"
                     /locus_tag="OHO85_003457"
     CDS             complement(6451..7191)
                     /gene="gltJ"
                     /locus_tag="OHO85_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCQ2672789.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7361..8269)
                     /gene="gltI"
                     /locus_tag="OHO85_003458"
     CDS             complement(7361..8269)
                     /gene="gltI"
                     /locus_tag="OHO85_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCQ2672790.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8724..10601
                     /locus_tag="OHO85_003459"
     CDS             8724..10601
                     /locus_tag="OHO85_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCQ2672791.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSNKPGAWV
                     MALSPDEKILLIPGMVRGDIVRINTITHQKESYPASDARGTISAMRFRPENGDVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            complement(10679..12217)
                     /gene="lnt"
                     /locus_tag="OHO85_003460"
     CDS             complement(10679..12217)
                     /gene="lnt"
                     /locus_tag="OHO85_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCQ2672792.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(12242..13120)
                     /gene="corC"
                     /locus_tag="OHO85_003461"
     CDS             complement(12242..13120)
                     /gene="corC"
                     /locus_tag="OHO85_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCQ2672793.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(13210..13677)
                     /gene="ybeY"
                     /locus_tag="OHO85_003462"
     CDS             complement(13210..13677)
                     /gene="ybeY"
                     /locus_tag="OHO85_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCQ2672794.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQKESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(13674..14714)
                     /locus_tag="OHO85_003463"
     CDS             complement(13674..14714)
                     /locus_tag="OHO85_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCQ2672795.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(14867..16291)
                     /gene="miaB"
                     /locus_tag="OHO85_003464"
     CDS             complement(14867..16291)
                     /gene="miaB"
                     /locus_tag="OHO85_003464"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCQ2672796.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMDLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            16437..17612
                     /gene="ubiF"
                     /locus_tag="OHO85_003465"
     CDS             16437..17612
                     /gene="ubiF"
                     /locus_tag="OHO85_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCQ2672797.1"
                     /translation="MTNQPTEIAIVGVGMVGGALALGLAQHGFTVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHDDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPILKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFMRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(17766..17840)
                     /locus_tag="OHO85_003466"
     tRNA            complement(17766..17840)
                     /locus_tag="OHO85_003466"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17806..17808),aa:Gln,seq:ctg)
     gene            complement(17878..17952)
                     /locus_tag="OHO85_003467"
     tRNA            complement(17878..17952)
                     /locus_tag="OHO85_003467"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17918..17920),aa:Gln,seq:ctg)
BASE COUNT         4410 a         4523 c         4688 g         4436 t
ORIGIN      
        1 agttaatgca cttaagggat tattaacaaa tgaataagga agaacaatat cttctttttg
       61 cgctttctgc gcccatggaa atacttaatc agggttgcaa acccgcgcat gactcgccaa
      121 aaatgtatac cgggataaaa gaattcgatc tctcatcatc ctggggaata aataatcgtg
      181 atgatttaat tcagactatt tatcagatga ctgatgatgg tcatgccaat gatttagctg
      241 gcttatatct gacatggcat cgctcttccc ctgaagagtg gaaagcatta attgctggtg
      301 gctcggagag agggttgatt tatacccaat tcgttgccca aaccgctatg tgctgtggag
      361 aagggggaat caaagcctgg gattatgttc gaatggggtt tctgtcacgg gtcggtgtgc
      421 tcaacaactg gctgacggaa gaagagagtt tatggctgca atcgcgcgtc tatgtacgag
      481 ctcaccatta ctatcacagc tggatgcact atttttccgc ctattcgtta ggacgtctct
      541 actggcaatc ttctcagtgc gaagacaaca cttcgctacg cgaggcgcta accctgtaca
      601 aatacgatag cgccgggagt cggatgttcg aagagctggc agcgggaagc gatcgtttct
      661 atgccacgct tccctggcag ccattaactg tccagcctga atgcccggta acgcttaagg
      721 atgtgagcga cctatgaaaa catgttggca aatcctcgaa attgaaagca cgacgcaaat
      781 agacattatc cgccaggctt atcttgctcg cttaccgtta tgtcatcccg aaaccgatcc
      841 gcaggggttt aaagcattac gccaggccta tgaagaggcg ctgcgactgg cggtaaatcc
      901 tgtcgaggaa gcagatgatg aagaaaaaga tgccgccgct gaacatgaaa tactgcgtgc
      961 attcaggaca ttactggatt cggaaagtga tcgttttcag ccctccgcct ggcagaaatt
     1021 tattcagcaa ttaaatacct ggaacatgga ggatgtcgat caattacgct ggccgctgtg
     1081 tgcaatcgct atagaagcgc gatatctttc attaaattgt gcttctttgc tggcagagcg
     1141 tttgaactgg cattcgttta atgacagcga aggaatggat gaggaagaaa gggaggcttt
     1201 tcttgaggcc attcaggctg gtgattgttt cgatttcctt agccttctgg aatatcccgt
     1261 tgcgttgcag aaccagactg ttgagtatta cttcgcgctg gaacgttgct gccgttacca
     1321 tcctgactac gtcactgcgt ttttggcgat ggaaggtccg tggttcattc ctgatgatgc
     1381 aaagttacat cgcaaactgt tgcgctggta cagctcggtg caaacaggta tggcggaact
     1441 cattcctgta gccaaacagt ggcaaatgga agaaccagaa agcgaagatg cccgatatta
     1501 cttgtgtgct caaaggttgt actgcggcga gggggaaagc ctgcttgccg atctctgcgc
     1561 gtaccgggaa agttacccat ctacacaagc tgataatttg ttgttgcagt ggagcaagcg
     1621 tcattgcccg gattatttcg cgttattagt gatggttatc gaagcgcgga gcatggtaga
     1681 tgcgcaaggt caaccgctga aatatgttcc tggtgagagc gcccggacgc ggctgttatg
     1741 ggcggaaatt ttacatagcg gaaaattatc gccgttaggt caatcgttta ttgagtcgtt
     1801 attcttcaaa cgcaaagcat gggcgtggtg gaaatcgaga gtcggtagcg agacagaaga
     1861 agattcaccg ttactggatt tgtatcgggt agcggaacag gtagtacttg aagcgtttcc
     1921 gaaacaagag atgctggccc gtcttaatac aaggctggaa ggcggagatg ctcatccatt
     1981 agaggccatt atcacccgga tgctgttgac gaaagtaaaa ctcgagccgg aggatgaaga
     2041 tgtcgatgag ccaacacctg aaaaccatga agaaaaaaat gatgagggtg aaaaaccaca
     2101 gagcattacc agcattatca aaatcagttt aacggtgctg gttataggtt atgctctcgg
     2161 caaaatcgcg atgttgttta gttgacgatg tgttttttgc gccgatgccg gatgcagcat
     2221 ttacgccgca tccggcagcc gtgattatga ggtctcaatg gctaaataaa cgctcgcccg
     2281 ccgcctgacc tcatcgacgc gggccatatc gtttgactgc aacgccgcat caaaatcggt
     2341 gatcacccgg ccaatctctt ctcgctcgtc acccagcgcc cgcgcccaca actcttccag
     2401 tttggcctta aaggtgcggt tgatgagcat atcgcgcgga taaattttca atgcggataa
     2461 ccgcgtccga ctctcttcaa tctgctgcgc gctcaatgtt accgggctat gattaatgac
     2521 tctggactta acgctgccat cttccagaag cacgtcaact tcaagcaagc cgttgatatc
     2581 ataactaaag cgaatatcaa tcgactgata agccccggtt ttcttcaacg gcacatcgaa
     2641 ggattcaacc agaatgttat ttttaacttt gtggttttct ccctgataga cgttaaccgt
     2701 aatagaatcc tgttccgggt gcatggtcga gtaagtttct acacgcgaca ccggtactgt
     2761 ggtgtttcgt tcaataattg gcgagaaaat gccggaaatg ccctggcggt taacttcaac
     2821 gcccaacgag taagggcaaa tatcagtgag gatcacctct tcaatatctt cactgcgtaa
     2881 gcggcaggcg gcctggattg ctgcgcccag cgcgacaatg gtgctcgggt cgtaactttg
     2941 atacggtaat ttgccaaaca gacgcacggc gattcgctgc accagcggca tttgtgacgc
     3001 gccgccaacc agtaccagac tgtcgatttg acttggcttc agacgcgcat cgcgcaacgc
     3061 ctgttcaatc ggcactcgca agcgattgag cagcggcaac cacaaatctt ccagttcatt
     3121 ctcataaaat tcgcattccc gtgcttcatc ctggtactgc cagcgaatgt gtaatggcga
     3181 ttgattgcta cattttgccg cttccacaca ggcatacaag gctgccagtt cactctcgtt
     3241 aagcgtggtc ctggtgacat ccgcgcgctt taaaacgtca tcgaccaaca tatgggtaaa
     3301 atcttcgcca ccgaggaagt tgtcgccagc ggaggcgtga acttcaatca ccggcgtggc
     3361 gtactcaagc accgtaacgt caaacgtgcc gccaccgaga tcaaacacca gcgaacgggt
     3421 attttgttgg gtatgcaggc catacgccat cgcagctgct gtgggttcat taattaagcg
     3481 taccgcattt aacccggcta actccgctgc taaacgggta tgcttgcgtt gttcatcgct
     3541 gaaataagcc ggaacggaga tcaccacatc tttaatcgga cgttgcagaa attcttcggc
     3601 atcttctttt aatgagcgta ataccaaaga ggacagttct ggcgcgttaa atgtgtcgct
     3661 gcctaaccgc cagttggtat tactgcccat tgcacgttta aataacgctg ccgttttatc
     3721 cggatgcgaa gtacgccgtg atacagccgg ttttccgact aaaatatgat tattttcatc
     3781 catgctaatt atggatggtg ttaaatattc accgaactta tttggaatta attgcgcggc
     3841 accgtctttc cagacggcaa ttaaactatt ggtagtaccg agatcgatac caatggcgag
     3901 ttctgcatta tccattgcat ttatatcttg aaagaaaaag agataaacag attaagacca
     3961 cccaggtaac gcgtggtgat cttaatcaat gacgtgtgtt aagcgtaaaa tttcagacga
     4021 tcagccagta aatcaacaaa gccctgacga tcaacatcga ccattacggt ggcattcggt
     4081 ttattgccag tcagataata ataatcaaca accgtcatac cctgagtata tttcccctgt
     4141 gtttccacgc caacccagcg ctcaacagtg gtaaacaact ccggtttcaa cagccaggcg
     4201 atggtgcatg ggtcatgcag cggtgcgccg acaaagcccc atttttcgtc tttatgatat
     4261 tcgaggaaga aatccagcag ttcggcaaca atggttgaaa cagggttgcc aatcgcgcgg
     4321 aaacgctcag tgtcttcaac gtggatttgt gctttgtgag taacatccag accagccatc
     4381 accaccggga tccctgactg gaagacaatt tctgccgctt ccgggtcaac gtaaatatta
     4441 aattcagccg caggcgtcca gttaccaagc cccattgcgc cgcccataat cacgatacgg
     4501 gcaattttgc tatgcagttc cggatggcta ttgagcagca aggcaacgtt agtttgcggt
     4561 ccggtagaca caatcgtgac aggttccgca ctttcacgca gcgttttcgc cattagctct
     4621 accgccgtac agttttgcgg tgcgaacgcc ggttccggta atgccgggcc gtcgagaccg
     4681 ctttcgccgt gcacattgtc cgcgataatc aactcacgca ttaacggttt taccgcgccg
     4741 cctgctaccg gaatatcggt gcgattgagc aaggtcagca tacgaagaac attgcgtaag
     4801 gttttttctg gtgtctggtt tccggcggaa gacgtaattg ctttgacatc aagctctggt
     4861 gaggcgaggg cgagaactat tgcgatagcg tcgtcatgac ctgggtcgca atctaacaga
     4921 attggcagtg ccattgttgc tccttgttgt gtgcttcttt gcgacaaggg taacgccagg
     4981 atgtaacaga tacgaggggc gaaacgataa agcgtgagat ggcgcgcact tgggtatgcg
     5041 cgccagagtg attaatgcag gattttcgcg aggaagtctt ttgcgcggtc cgatttcgga
     5101 tcatcgaaga aagcgtcttt cggcgagtct tcgacaattt taccctcatc cataaagatc
     5161 acccgattcg ccactttacg ggcaaagccc atttcgtggg tcaccaccat catggtcatt
     5221 ccttcgttcg ccagttccac catcacgtcc agcacttcgt tgatcatctc cggatccagc
     5281 gccgacgtcg gttcgtcgaa cagcatcgca ataggatcca tacacaatgc gcgagcgatc
     5341 gccacacgct gctgctgacc gccggaaagc tgcgccggaa atttattggc atgtgcggaa
     5401 agtcccacgc gctccagcaa tttcaatgct ttttcacgag ccggcgcttt atcgcgttta
     5461 agcactttca cctgcgccag ggtcaggttt tcgataatcg acagatgagg gaacagctcg
     5521 aaatgctgga ataccatccc gacgcgggaa cgcagctttg ccagatcggt tttcttgtca
     5581 ttaaccacga taccatcgac ggtgatttca ccttgctgca ctggttcgag gccgttgacg
     5641 gttttaatca gcgttgattt gccggaacca gacgggccgc aaaccaccac cacttcgcct
     5701 tttttcactt cggttgagca gtcggtcagc acctgaaagt gaccatacca ttttgaaaca
     5761 tttttcaggg taatcattat gctgtccttc ttttcaagta gctgaccaac aacgacgcgc
     5821 taagactaat aacgaaataa acaaatccgg caaacaggat catctcaacc tgcgtaccat
     5881 cacgctcacc aatggttgag gcggtacgga agaaatcggc cagagataac acatacacca
     5941 gcgaggtatc ctggaacagt acgatgccct gagtgagcag cagcggcacc atcgcgcgga
     6001 acgcctgcgg cagaataatc agtttcatcg actgccagtg agtcattccc aacgccagcg
     6061 cggcgctcga ctgaccacga gaaatacttt gaataccagc acggataatc tctgaatagt
     6121 aggccgcttc aaacatcgaa aacgccacca tcgccgaaat taaacggata tcattttttg
     6181 gtgataatcc cagcacgttt tgcagaaaac ccggcacaat caggtaaaac cacagcaaaa
     6241 ccataactaa aggaatcgag cggaatacgt taacgtaggc tttggcaaac cacgctacag
     6301 gcgcaaagct ggacaagcgc atcaccgcca gcatggtgcc ccacaaaata ccaatcacga
     6361 ctgccgtgac ggtgattttc agggtgatca ccagcccgtc gagcagatat ggcagggaag
     6421 ggacaatgga actccagtca aactcgtaca ttatttaccc cccatgttgc caggcaggcg
     6481 aactttacgt tcaaccagcg tcatcaccag catgataaaa gcgttaatca atacatacgc
     6541 cagcgtgatg gcggtaaacg actcccaggc gtgggctgag taatcaagca atttacccgc
     6601 ctgcgccgcc atatccacca gaccaatagt cgaggcgatg gcggagtttt tcaccaggtt
     6661 catcatctct gaggtcatcg gcgggacgat aacgcgataa gcattaggca gcagtacgta
     6721 tcgataagcc tgcggtagcg tcaggcccat cgccagcgcg gcattctttt gcccacgcgg
     6781 cagcgactga atagcggcgc gtacctgttc gcaaacacgg gcggcggtaa acagccccag
     6841 gcagagcatg gatgaaagga aaaactgaat attgggatcc agctcggcct taaaccacat
     6901 gccgattttc tccggcagca gctccgggat caccagatac caggtaaaga attgcacaat
     6961 caacggcacg ttgcggaaca gttcgacata caacgtaccc agaccggaga gaaaacggtt
     7021 tggaacggta cgtaaaatgc cgaaaaatga accgacgagg aaagcgataa tccaggcgca
     7081 gatcgacaaa gcgatcgtca cctgaaaacc actccagatc caaccaagat aggtggtgtt
     7141 gccgaacggg gcttgttgta aaaaaatacc ccagttccag tctatagaca taaatctact
     7201 ccagaaaaaa gagggtagca gcgttaactg ctaccctcga agattgttac ccagcgtatt
     7261 gcggttttca ggcccgatgg ggaacgacca tcaggcgtat agtctgtccg tgctacgtaa
     7321 caatcgagag ggctggaatt tccgcccctg gttcttgtaa ttagttcagt gccttgtcat
     7381 tcggttcttt gaacagtgct ttcatttcgt ctgacagttc gaaattcatg ttcaggtttt
     7441 tcggcggaat tggatttttg aaccatttat caaaccattt ttccgcttca ccggaggtct
     7501 gcacctgagc gatagtgtca tccatcagct ttttgaactg cggatcgtct ttacgcaaca
     7561 tgcaaccgta ggcttcctga gactgcggct tgccgacgat atcccagttg tctggtttct
     7621 tcgctttcgc acgttcaccg gccagcagag cgtcatccat cataaaggca acggcacgac
     7681 cgctttccag ggtgcggaaa gagtcaccgt gatctttggc gctgatgatg cgcatattca
     7741 ttttttgctc ttcattcagt ttgttgagca aaacttcaga ggtagtgccg gaagtgacga
     7801 ctacggcttt gtctttcagg tcggcaaaat ctttgatatc gccacccttt ttggtcagca
     7861 ggcgcgtacc gaccacgaaa atagtgtcag agaaagccgc ctgtttttgg cgttcgacgt
     7921 tgttagtggt agaaccacat tcaaaatcga aagtgccgtt ttgcagcagt ggaatacggt
     7981 tttgtgaggt aatcggaatc agtttcacct gcaagtccgg tttgttgagt ttctttttca
     8041 ctgcttcaac aatggcgttg gagtaatcct gcgagtaacc caccactttt tgctgattgt
     8101 cgtaataaga gaaaggtact gaagattcac ggtgaccgac gacaatcaca ccgtttttgg
     8161 cgattttgtc cagcgtactg cctgctgccg gggcggcgtc atctgcctgt gccagtcctg
     8221 cggaaagcgc cagggcgagg attgctgtgg caggtttacg taattgcata tccaactcct
     8281 ttatcttctg cgttaagaac gcatggatac ccgttgtgag tgtttgtgtt gttatcgtct
     8341 gcaactttat tgtgcagtgt tgtgcctgtt aggtaacatt tagtttggct aaatgtaaag
     8401 atattgctgt tttattgttt gtttttgcga gatgcgccgc accattccga agcaaaattc
     8461 ttaaaccgca cctttttggt gctaccgctg atttgtggtg gtgcaacctg gttgcgtcgc
     8521 ggctgatttc atcgcttaca cagtaaataa agcaacttac atgccaaaaa atacttaggg
     8581 gcgaattaat tacgattgta attttaggct gaaaaagtca tatttcggac agaattatct
     8641 tttcggcatg ggacgtttgc cgggatgata actgcacacg ggaaaaatgc tttttacctg
     8701 tcttttcaag gaagaaacac cttatgtctg catcttcggt taagccgtta aatgttcaat
     8761 tacccgcaat aacccttatc ctttttgcac tctgtattgg gatattttgt tatctcgcac
     8821 aatggatgag ttatgaagag gtcgatcaat ccgcacttat ccatctcggc gcaaacgttg
     8881 cgcctcttac tttgtcgggt gaaccctggc gcttattgag cagtatcttt ctgcacagta
     8941 gtgtttctca tctgctgatg aatatgtttg cattcctggt cgtgggaggc gtggcggaac
     9001 agattctggg gaaatggcga ctcctgatta cctggttatt ctccggcgtc tttggtgggc
     9061 tcatcagcgc ctgttatgcg ttacgcgaaa gtgagcagat agtcatcagc gttggggcat
     9121 ccggggcgat tctgggaata gctggtgcag cgatagcaac acagtttgct tcaggtgcgg
     9181 gcacatacca taaaaaccag aggcgagtat tttctctgtt gggtatggtg gcgctgacac
     9241 tgttgtatgg tgcccggcaa acaggaatag ataatgcttg tcacattggc ggcctgattg
     9301 cgggtggcgc gttgggttgg ctgagcgcgc gtttatctgg gcaaaaccga ctcgttacgg
     9361 aaggcgggat tattgttgcg ggcagtcttc ttctgaccgg ggctatctgg cttgcgcagc
     9421 agcagatgga tgagtcagtt ttacaagtca ggcaaagcct gcgtgaagcg ttttatccac
     9481 aggagattga acaagagcga cggcaaaaaa agcagcagtt agcggaggaa cgcaacgccc
     9541 tcagggaaac attatccgct ccggtaagtc gtgaacaggc cagtggtgat ttgctcgctg
     9601 agattgccga tatccatgat atggcgatca gtcgggatgg taatatgttg tatgccgcaa
     9661 ttgaaaacac gaacagcatt gttgttttcg acctcggaca aaagaaaatc ctgcatacct
     9721 ttacagcgcc catagcgaaa gaaaagtcag tcaaacattg tggtggctgt aaagatcagg
     9781 gcgtcagatc gctggcatta agcccggatg aaaagttgat ttatgcgact tcatttgaag
     9841 cgaatgcgtt atcggtcatt aacgtggcga caggggagat tattcagtcg attaccaccg
     9901 gtgcacatcc tgacagcctt atcctctcgc gtgatggcac aaaagcctgg gtgatgaatc
     9961 gcaccagtaa tagtgtgtca gcgattgatc tggtgactta tcagcatgtg gcggatatcc
    10021 cgctggagaa atacgacggg acggggacga gtaataaacc tggtgcctgg gttatggctc
    10081 tttccccgga tgaaaaaata ttgttgatac ccggtatggt cagaggtgac attgtacgca
    10141 tcaataccat cacgcatcag aaagaaagct atccggctag tgatgcgcga ggaacgatat
    10201 cggcgatgcg ttttcgacct gaaaacgggg atgtaatttt tgccgacagc caggggattt
    10261 cacgtataag cgtaggggat caacaagcca gcattatgac gcaatggtgt agcaggagcg
    10321 tttattccgt tgagggtatt agcccggacg gtcagtattt agcgttggtg tcatatggcc
    10381 tgcaaggtta tgtcatcctg ctcaatatta atgccgggca gattattggc gtttatcctg
    10441 ccagctacgt taatcacctt cgtttttcag cggatggtag aaaaatattt gttatggcga
    10501 agaacgggtt gatccaactg gacaggacgc gctcgcttga tccgcaggca attattcgtc
    10561 atccccaata tggcaatgtg gcttgtatcc ctgaaccgta agaaaaaggg gcatgataac
    10621 catgccccaa agctcaatga gaaagtacgt tttgctcatg caggatacat caggcacatc
    10681 aacgtttacg tgctcgcaga ctcatcagca cagcggcaaa accaaacagt gccgtcagca
    10741 cccacagcgg ccagttgccg gttcgtgcat acggtgtgag tccggtggtc ggcgtcacgt
    10801 tagtggtcaa cacctcgcgg gtgaactgcg ggatcatcgc ctgaatctca ccctgcgggc
    10861 caatcaccgc cgtaatgccg ttgttggtgc tgcgcaacag tgggcgcgcc agctccagcg
    10921 cacgcattcg cgccatctgg aagtgttgcc atggaccaat agatttacca aaccacgcat
    10981 cgttggagat agtcagcaga tagtcggtat ccgggcggaa gttatcgcgc acttgctcgc
    11041 caagaatgat ctcgtagcaa atggccgcag taagctcaat accatttacc gacagcggcg
    11101 gctggatata tggcccacgg ctgaacgacg acatcggcag atcaaagaac ggtgctaacg
    11161 gacgcagaat cgactccagc gggacaaact cgccaaacgg caccagatgg tttttgttat
    11221 agcgatcggc tgattcgtag ctgtacggtg cacctttacc cagtgtgatg atggtgttgt
    11281 aggtatcgta gcggttctgc ttattgagac gcgcgtcgac aatcccggtt acgagcgagc
    11341 tacctttatc gcgcaactca ccgtccagtg ctttgaggaa tggttgctga ttaatttcca
    11401 gatcggttat cgccgactcc ggccagataa tcaacgatga tttgcccatc agcggagccg
    11461 ttgcgttgta gtaaatcttc agcgtattaa gaagctgatc tccatcccat ttcagtgatt
    11521 gcggaatatc gccctgaacc atcgaaacct gaatggtttt ctccggttgt ggggtaaacc
    11581 attggatgta acgcagcggg aagggtaggg caaacagcac gacggccacc accagcggac
    11641 gccagttgcg tttgaccaac gccagtgcca gcaggccact aaccatcatc agcaggaagt
    11701 taatggcttc cacgcccatt atcggtgcca gcccttttaa cgggccatca atctggctat
    11761 agccgaactg taaccacggg aagcctgtca gtacccaacc gcgcagaaac tcggtcactt
    11821 gccagagaga aggggcggca atcgccacgc gcagccaggt ggttttcggc cacagacgcg
    11881 acagcacgcc agcaaacagt ccggtataca gcgacaaata cgccgccagc agcaccacca
    11941 ggaagatgtt aaccgggcca ggcatcccgc caaaggtcgc gatgctgaca tagacccagt
    12001 taataccgct gccaaagagg ccaaatcccc agcaaaagcc aatagcggca gactggagtg
    12061 ggcgacggtt aaaggtcaac gcctgaagcc ccatcagcga aataatcgcc gcaggccaga
    12121 cgtcgtaagg agagaaggcc agcgttccgc aggcaccgaa taataacgcc agcagcaggc
    12181 gaatgcgctg gcgttcaatt aatgaggcaa aagccatgta gttatctatc cagtttcggt
    12241 tttattcatc cagctttggc tggggtgagt catccgggat tttgacatga acctgaataa
    12301 tacgccgact gtcggccatc gccactttga actggtaacc gtcgatgtcg atagtttcgc
    12361 cacgcgccgg aagatgccca aatgcctgca tcaccagacc accgatagtg tcaacctctt
    12421 catcgctaaa gtgggtgccg aacgcttcgt tgaagtcttc aatggaagcc agtgcgcgca
    12481 cggtccaggt atgacgactc agctgacgga agtcgatatc atcttcttcg tcatactcgt
    12541 cttcaatctc accaacaatt agttccagga tgtcttcaat ggtcaccaga ccggaaaccc
    12601 caccgaattc gtcaatgacg atcgccatgt ggtaacgctg agagcgaaac tctttcagca
    12661 tccggtctac gcgcttactt tcaggaacga caaccgcctg acgtaacact ttgtccatgc
    12721 tgaaggcttc agcatcgctg cgcataaacg gcagcaagtc tttcgccatc agaatccctt
    12781 caatgtgatc tttgtcttcg ctaatcaccg ggaaacgtga gtgggcggac tcgatgatga
    12841 catcaaggca ttcgtccagc gtctggttgc gtttcagggt aatcatctgg gagcggggga
    12901 tcatgatgtc gcgaacgcgt tggtctgcga tgtccatcac cccttcgagc atatcgcgcg
    12961 tatcttcgtc gataaggtcg ttctgcccgg aatcacggat cagcgccagc agttcgtcac
    13021 ggtttttcgg ttcaccgtgg aaaagttggc tgagtaacag ggagaaaaat cccttcttgt
    13081 tgcttatcgt gtcactactg tgtgaattgt cgtcgctcat ggcgtcgtat gggttctcat
    13141 gttagttaat caaaacgccg tcgttaatca ccaacggcgg ggacgtctgc cagtcaaatg
    13201 cctggcaaat tattctttct cggcaatgta cggatcctca tagcccagag caagcataat
    13261 ctctgtttcg agggcttcca tttcttctgc ttcgtcatct tcgatgtgat cgtaacctaa
    13321 caaatgcaga ctgccgtgca ccaccatatg cgcccagtgc gcctccagtg gtttgccttg
    13381 ctcctgagct tccttctcaa ccacctgacg gcagataacc agatcgccca gtagcgacat
    13441 ctccatgcca ggcggcactt caaacgggaa ggagagcacg ttggtcggct tatccttacc
    13501 gcgataggtc agattcagac tgtggctttc ggcggtatcg accacgcgaa tagtcacttc
    13561 cgattctttc tgaaactgcg ggatcaccgc attcagccat gtctgaaact ggctctcttc
    13621 cggtaacccg gaattatctt cacatgccag ttgtaaatcg aggatcacct gactcatttt
    13681 tgttcctgtt cttcgcgctt gcgttctgcc gccagcgccg cttttcgttt ttgttctgct
    13741 tcttcccagg cttcataagc gttaacgata cgcgccacca ccgggtgacg aaccacgtct
    13801 tcgctgtgga agaagttaaa gctgatctct tcgacatcgg ccagcacttc gatggcatga
    13861 cgcaaacctg atttagtatt acgcggcagg tcgatctgtg tgacgtcgcc ggtgataacc
    13921 gcttttgagt taaaaccgat acgggtcagg aacatcttca tctgttcgat ggtggtgttc
    13981 tggctctcat cgagaatgat aaaggcgtca ttcagcgtac gaccacgcat ataggccaga
    14041 ggcgcgactt caataacgtt gcgctcaatc agtttctcga ctttctcaaa gcccagcatt
    14101 tcaaacagcg cgtcgtacag tgggcgcaga tacgggtcta ctttctggct taaatcgcca
    14161 ggcaggaagc ccagtttctc accggcttct actgccggac gagtcagcag aatacggcga
    14221 atttcctgac gctccagggc atcaactgcc gcagccactg ccaggtaggt tttacccgta
    14281 cccgccgggc caacgccgaa ggtaatgtca tggtcgagaa tattggcgat gtactgcgcc
    14341 tggtttggcg tgcgcggctt aattacgccg cgtttagttt tgatattgac cgctttgccg
    14401 tactccggca cgctctccgc gctttgctcc agtacccgcg cttctttaat cgcaaggtgg
    14461 atctgttccg gttcgatatc ctgaatctga ccgcgcatcg gggcagtatc gacatacagg
    14521 ctacgcagaa tgtctgccgc agcggtgacg caaatcggac ggccggtcag tttaaagtgg
    14581 ttatcgcggc gattgatctc gatgccgaga cgacgttcga gctgcttgat gttgtcatca
    14641 aacgggccgc acaggctcaa cagacgcgca ttgtctgctg gctccagggt gatttcgcga
    14701 gtgtctatgt tcaaaccgtt cctcttatct gtacgccgcc ggaagctgaa cattcaccgg
    14761 cctataagga aattattcac gccacaggaa aaaggcgcaa gcattgcgat aaagatgggg
    14821 ataaagagag aaaaaacaag gcccaccgga acggcgggcc tgagaattac ggctgataat
    14881 aacccacgcc aaggtcattt tctttgcgag tacgggcaat caccgattcg ggtgtttctg
    14941 ccacgcgcag atccatttca tcttcagtac gcaccacttt accgcgcaga gagttcgggt
    15001 agacgtcggt aatttctaca tcgacgaatt taccgatcat atccggtgtg ccctcgaagt
    15061 tgaccacacg gttattttcg gtacgcccgg aaagctccat gatgctctta cgcgatgtac
    15121 cttctaccag aatacgctgg gtggtgccga gcatccgacg gctccatgcc atcgcttgct
    15181 gattgatacg ttcttgcaga atatacagac gctgcttctt ctcttcttcc ggaacatcat
    15241 caaccatatc agcggcaggc gttcctggac gtgcagagaa gataaagctg tagctcatgt
    15301 cgaaattgac gtcggcaatc agcttcatcg ttttctcgaa gtcttcggtg gtttctccag
    15361 ggaagccaac gatgaagtca gaactgatct gaatatccgg acgcgccgca cgcagtttac
    15421 ggatgatcgc tttgtactcc agcgccgtat gggtacggcc catcaggttc agaatgcgat
    15481 cggaaccgct ctgtaccggc agatgcagga agctcaccag ctccggcgtg tcgcgataca
    15541 cttcgatgat atcgtcggtg aattcgatcg gatggctggt ggtaaagcga atacgatcga
    15601 tcccgtcgat tgccgcaacc agacgcagca gatcggcaaa cgatccagtg gtgccgtcgt
    15661 agttttcacc acgccaggcg ttcacgttct gaccgagcag gttgacttca cgcacgccct
    15721 gagccgcaag ctgggcaatt tcaaacagaa tatcgtcgga cggacggctt acctcttcac
    15781 cacgggtgta aggcaccacg cagtaggtgc aatatttatt gcagccttcc atgatggaga
    15841 caaacgcggt cggcccttcg gcgcgcggtt ccggcagacg gtcaaacttc tcgatttccg
    15901 ggaagctgat atctacaacc gggctgcggt cgccgcgcac ggagttgatc atctccggca
    15961 gacgatgcag cgtttgcggc ccaaaaataa tatcgacata gtgggcgcgc tggcgaatgt
    16021 gctcgccttc ttgcgatgcc acgcagccac cgacgccgat aatcaggtct ggattcttct
    16081 cttttaacag tttccagcga cccaactgat ggaagacttt ttcctgagcc ttctcgcgga
    16141 ttgagcaggt gttcagcagc agcacatccg cttcttccgc cacgtcggtc agttgatagc
    16201 cgtgggtggc atccagcaga tcggccatct tcgatgaatc gtactcgttc atctgacagc
    16261 cccaggtttt aatatggagt tttttggtca tcgacttgct cttgcgaaat agtagccagg
    16321 aatgcagggc gcatagtgta atgctttgct gccgttgtga ccagtatgag cgttatcagc
    16381 ccttgggggt aaaaatcctg taaacttaaa gcatattgct aacaggatga ttgaccatga
    16441 caaatcaacc aacggaaatt gccattgtcg gcgtaggaat ggtcggcggc gcactggcgc
    16501 tggggctggc acagcacgga tttacggtaa cggtgatcga gcacgcagaa cctgcaccgt
    16561 ttgtcgctga tagccaaccg gatgtgcgga tctcggcgat cagcgcggct tcggtatcat
    16621 tgcttaaagg gttaggggtc tgggatgcag tacaggctat gcgttgccat ccttaccgca
    16681 gactggaaac gtgggagtgg gaaacggcgc atgtggtgtt tgacgccgct gaacttaagc
    16741 taccgttgct tggctatatg gtggaaaaca ccgtcctgca acaggcgctg tggcaggcgc
    16801 tggaagcgca tccgaaagta acgttacgtg tgccaggctc actgattgcg ctgcatcgcc
    16861 atgatgatct tcaggagctg gaactgaagg gtggagaaac gatccgcgcg aagctggtga
    16921 ttggtgccga cggcgcaaat tcgcaggtgc ggcagatggc gggtattggc gttcatgcct
    16981 ggcagtatgc gcagtcgtgt atgttgatta gcgtacagtg tgagaacgat cccggcgaca
    17041 gcacctggca gcaatttact ccggacggac cgcgtgcgtt tctgccgttg tttgataact
    17101 gggcatcgct ggtgtggtat gactctccgg cgcgtattcg ccagttgcag aatatgaaca
    17161 tggcgcagtt acaggcggaa atcgcgaagc atttcccgtc gcgtctgggt tacgttacac
    17221 cgcttgccgc tggtgcgttt ccgctgacgc gtcgccatgc gttgcagtat gtgcagccag
    17281 ggcttgcgct ggtgggcgat gccgcgcata ccatccatcc gctggcgggg cagggggtga
    17341 atcttggtta tcgtgatgtc gatgccctga ttgatgttct ggtcaacgcc cgcagctacg
    17401 gcgaagcgtg ggccagttat ccaatcctca agcgttacca gatgcggcgc atggcggata
    17461 acttcattat gcaaagcggt atggatctgt tttatgccgg attcagcaat aatctgccac
    17521 cactgcgttt tatgcgtaat ctcgggttaa tggcggcgga gcgtgctggc gtgttgaaac
    17581 gtcaggcgct gaaatatgcg ttagggttgt agccttacaa cattgccgga tgcgtgccaa
    17641 ccgtaggtcg gataagacgc ggcagcgtcg catccgacat tgaaggataa gacgtgtcaa
    17701 catcgcattc gacattgaat gaacgcagaa aagcaaaaag ctcgccgaag cgagcttttt
    17761 taatgtggct ggggtacgag gattcgaacc tcggaatgcc ggaatcagaa tccggtgcct
    17821 taccgcttgg cgatacccca actgggtgca cttacgatgt aagcgtcttg aataaattgg
    17881 ctggggtacg aggattcgaa cctcggaatg ccggaatcag aatccggtgc cttaccgctt
    17941 ggcgataccc caacaaattg gtttttgaat ttgccgaacg tattcgacac attcagaatt
    18001 tggtggctac gacgggattc gaacctgtga ccccatcatt atgagtgatg tgctcta
//
LOCUS       DAJOWB010000095        17792 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJOWB010000095 DAJOWB010000000
VERSION     DAJOWB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.095"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            133..636
                     /locus_tag="OHO85_003468"
     CDS             133..636
                     /locus_tag="OHO85_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HCQ2672798.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(703..2160)
                     /gene="nuoN"
                     /locus_tag="OHO85_003469"
     CDS             complement(703..2160)
                     /gene="nuoN"
                     /locus_tag="OHO85_003469"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCQ2672799.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYVPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2167..3696)
                     /gene="nuoM"
                     /locus_tag="OHO85_003470"
     CDS             complement(2167..3696)
                     /gene="nuoM"
                     /locus_tag="OHO85_003470"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCQ2672800.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3860..5701)
                     /gene="nuoL"
                     /locus_tag="OHO85_003471"
     CDS             complement(3860..5701)
                     /gene="nuoL"
                     /locus_tag="OHO85_003471"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCQ2672801.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5698..6000)
                     /gene="nuoK"
                     /locus_tag="OHO85_003472"
     CDS             complement(5698..6000)
                     /gene="nuoK"
                     /locus_tag="OHO85_003472"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCQ2672802.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5997..6551)
                     /gene="nuoJ"
                     /locus_tag="OHO85_003473"
     CDS             complement(5997..6551)
                     /gene="nuoJ"
                     /locus_tag="OHO85_003473"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCQ2672803.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6563..7105)
                     /gene="nuoI"
                     /locus_tag="OHO85_003474"
     CDS             complement(6563..7105)
                     /gene="nuoI"
                     /locus_tag="OHO85_003474"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCQ2672804.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7120..8097)
                     /gene="nuoH"
                     /locus_tag="OHO85_003475"
     CDS             complement(7120..8097)
                     /gene="nuoH"
                     /locus_tag="OHO85_003475"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCQ2672805.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8094..10820)
                     /gene="nuoG"
                     /locus_tag="OHO85_003476"
     CDS             complement(8094..10820)
                     /gene="nuoG"
                     /locus_tag="OHO85_003476"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCQ2672806.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQEHLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10873..12210)
                     /gene="nuoF"
                     /locus_tag="OHO85_003477"
     CDS             complement(10873..12210)
                     /gene="nuoF"
                     /locus_tag="OHO85_003477"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCQ2672807.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12207..12707)
                     /gene="nuoE"
                     /locus_tag="OHO85_003478"
     CDS             complement(12207..12707)
                     /gene="nuoE"
                     /locus_tag="OHO85_003478"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCQ2672808.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12710..14512)
                     /gene="nuoC"
                     /locus_tag="OHO85_003479"
     CDS             complement(12710..14512)
                     /gene="nuoC"
                     /locus_tag="OHO85_003479"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCQ2672809.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14606..15268)
                     /gene="nuoB"
                     /locus_tag="OHO85_003480"
     CDS             complement(14606..15268)
                     /gene="nuoB"
                     /locus_tag="OHO85_003480"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCQ2672810.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15284..15727)
                     /gene="nuoA"
                     /locus_tag="OHO85_003481"
     CDS             complement(15284..15727)
                     /gene="nuoA"
                     /locus_tag="OHO85_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCQ2672811.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16357..17295)
                     /gene="lrhA"
                     /locus_tag="OHO85_003482"
     CDS             complement(16357..17295)
                     /gene="lrhA"
                     /locus_tag="OHO85_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCQ2672812.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4445 a         4950 c         4606 g         3791 t
ORIGIN      
        1 gttataaatt tgttatacaa cagtaaataa taaaaatttc tcgaggcggg tccagaaaaa
       61 gcgacatgca cattttattt tccctcgtct gttttaagat gcgaaacatg aaattcaatg
      121 aaggaaaaat aaatgggaat gattggttat tttgcagaaa ttgactcaga aaagattaac
      181 caactgcttg agtctactga aaaacctttg atggataata tacatgacac gctttcagga
      241 ttacgccgac tggatattga taaacgctgg gattttttac actttgggct gacaggcacc
      301 tctgcttttg atcccgccaa gaatgatccg ctgagtcgtg ccgtgcttgg tgaacacagc
      361 ctcgaagatg gcatagatgg cttcctggga ttgacctgga atcaggagtt agccgcgaca
      421 atcgaccgac tggaatcact ggatcgaagt gagctgcgta aacagttttc cattaagcga
      481 ctcaatgaaa tggagattta tccgggagtg accttttcag aagagttaga ggggcaactc
      541 tttgcctcga tcatgcttga tatggaaaag ctgattagcg cgtatcgacg tatgctgcga
      601 caagggaatc atgcgctgac ggttattgtt gggtgatggt gtcagagcgc caaaaaaaac
      661 cgccgagcat gtcggcggct ttctgactta caaactaaca gattacatca gcggcattgc
      721 taaccgcaca atgctaatca gcggttgtgg ccatacaccc agcaccagta ccaacagtgc
      781 agaaatcagc accactatac cgcccgcgct gtactgccag tttgatggtg catcgcgacc
      841 cggttgttcc ggggcgtgaa gatacaggct caccgccacg cgcaggtagt agtagaggcc
      901 gattgccgaa ccgacaacca cggcacccac cagccaccac aagtgtgcct ggacaccgac
      961 cgccagcacg tagaacttac cgataaagcc cagcgtcatc gggataccgg ccagagacag
     1021 catcatcacc gtcatcactg ccgcgaggat cggacgatgc cagaacagac cgcggtaaga
     1081 gaacagggaa tcagcatccg ggccacgata cgggctggac atcaggctga ccacgccgaa
     1141 cgcgccgagg ctgctgaaca gataaccggc caggtaaacc cctaccgctt ccatcgacat
     1201 ctcaccagtt tgcagcgcaa tcagcgccac cagcagatag ccgaggtgag agatagatga
     1261 gtaaccgagc agacgtttga tgttggtctg gctcagcgcc atcaggttac cgaagatgat
     1321 ggaggcaaag gcgataatcg ccagtactac gcgaatcgct tcgctgtcac ccaccggtac
     1381 gtagaggaac agacgcatca ccacaccgaa gatagcgatt ttgctcgccg tcgccaggaa
     1441 agtggaaacc ggcgcaggcg caccctggta tacgtctggc gtccacaggt ggaacggcac
     1501 cagcgagagt ttgaagccga ggccaacaat catcagtccg aaacctgcca gcaacagcgg
     1561 ctcgttgagc ataccgtcgc cgaggttttt acccagcgcg acaaacgaca ggtcgccaga
     1621 ctgtgcatac accagcgcca taccaaacag caggaaagaa gacgctgcgg cagaaaggat
     1681 ggtgtatttg atactggctt ccagcgaacg tttctggcgg aaagcgtaac cgaccaggcc
     1741 aaacagcggc aaagagatca gttcaatacc gaggaacaaa gacgccagat ggttggcatt
     1801 cgccagcagg atcccgccca gcgcggcaat taacaccaac aggtagaact catccttgtt
     1861 gtcgttatag ccttcaagcc acgggtaggc gaaagtacag gtggcgaggc tcgccaacaa
     1921 taccagcccg gtgtaaagca tggcgaaacc atcaacgcgc atcagcggcg taacgtccat
     1981 agcgcccgcc tggccgacaa accagagcga aaccagcgcc gcgttaagcc caataaccga
     2041 gagcgtagcg ttgaggaaat gattgcgtcg ccacgcaatg gagagcatca caaccaccac
     2101 cgtcaagccg acgatcagca acggtagcag tgcgatcagg ttttgtggag ttattgtcat
     2161 ggcgatttac ggccttgtag tagtaacgga attaacaaac cactgctgga tattgccaat
     2221 cgcggagtgc gaggtatcca gaatcggctg cggatagaag cccagcagta ccagcagcac
     2281 caccagcaac aggatcataa acagctcacg cagcgacatc cctggcagtt cctggctggc
     2341 aatctggctt ttcgctttac cgaagtaagc gcgatgcaac atcgccagcg aataaacaga
     2401 tgcaaagacc agcccaaagg tagagataac ggtaatcacc gggacaacct ggaagctgcc
     2461 gaacagaatc ataaattcgc cgacgaagtt accggtgcca ggcatcccaa gcgttgccac
     2521 cgcaaagaac agcgacagtg ctggcagcca tttcatcttg ctccacagac cgcccatcat
     2581 gcgcatgtcg cgggtatgga tacgttcata aagctgacca caaagaataa acagacccgc
     2641 cgccgacaag ccgtgcgcaa tcatctggat taccgcgccc tggtaggcca actggctgcc
     2701 ggtgtagata gcaatcagca cgaagcccat gtgggaaacc gaggtgtagg cgatcagacg
     2761 tttgatatcg gtctgggcga aagccatcca cgcaccgtag aagatgccga taacccccag
     2821 ccacatagcg attggcgcaa actctgccga tgcgttcggg aacagcggca gggagaaacg
     2881 cagcaaaccg taagcggcag ttttcagcaa gatccccgcg aggtcaacgg aaccggcggt
     2941 cggagcctga gagtgcgcat ccggtagcca gccatgcagc ggaaccaccg gcattttgac
     3001 tgcgaaggcg atgaagaagc ccagcattaa caggtattcc acaccactgg acattggcgt
     3061 attcagcagc tcttcatagt tgaaggtcca gacgccggtc gcattgtagt gaacaaaaac
     3121 cagcgccagg atggcaatca acatcaccag gccactcgcc tgggtgtaaa tgaagaactt
     3181 ggttgccgcc gtgatacgcg ttttaccgtc agaggcttta tgcccccaca gtgcgatcag
     3241 gaagtacatc ggcaccagca tcatttccca gaagaagaag aacaggaaca tgtcgatggc
     3301 aaggaacacg ccgataacgc cgcccaggat ccacatcagg ttgaggtgga agaagccctg
     3361 atatttttcg atctctttcc acgaacatag taccgccagc acaccgagca gaccggtcag
     3421 cacgaccatc agcagcgaca gcccgtcaat ggcgagatga atagagatac caaaacgcgg
     3481 gatccacggc atgtcgaatt cagactgcca ctgcggaatt ccggcggatt gcgtcagtga
     3541 ataaccgccc tgcaaccaca gttgcagcga cagcgccagc gtcaatccca tggtgatcag
     3601 cgcgatccag cgtggcacct tgacgccaaa gcgttcggtc tgccagcaca ggaagccgcc
     3661 aataaaggga attaatatta gccagggtag taacatggcg atctttattc cttgtaaaag
     3721 tcccgtcagg accggatttt caacgaattc tcacgacaga aaattctctt cggactgggg
     3781 gtagtgtcgg atgcaccgcg aaggcggctt atccgaccta cgttcttata tgcccagggg
     3841 caatcccaca atccttaact caacgcagta ccatcaacag tgccagcacc acgaccgcac
     3901 cgatgctcat ggatgccaca taccagcgca gataaccgtt ctcgcttaac agcagacctt
     3961 tacctgcaaa gcgggaaaga accgccggga tgttcatcat tgagttcagc ggatcgcgtt
     4021 tcagcaacca ggcaataccc aggaacggtt tgacgaacac tttgtcgtac agccagtcaa
     4081 agccccaggc gttgtaccac caggtgccca gcagacggcc cggcgcactg ttggcaatgg
     4141 aggtcaccag agtacgttta cccagccaca gccaggctgc cagcagaatg ccgaccaccg
     4201 cgaccacgcc agaggtaatt tccagggtca acatgctgcc gtgcgccagc tccgtcgttt
     4261 gcggaagcac gccctgcagc ggcggtacaa tcagtgcgcc aacgaaggtg gaaaggatca
     4321 gcagcacaat cagcggcagg ctgtgagtta cccctttcac ggcgtgagcg tgaatttgtt
     4381 cttttccgtg gaagacgatg aaaatcatac ggaaggtgta gagcgaggtc ataaacgcac
     4441 cgaccagacc tgccaccatc agattgatat gaccattcgc catcgcaccc gcgaggatct
     4501 catccttact gaagaagcca gcagtgacca gcggcagcgc cgacagtgct gcgccgccta
     4561 ccaggaagca gagataaacc agcggaatag atttacgcag accgcccatc ttgaagatgt
     4621 tctgttcgtg atggcaggcc agaatgacgg aaccggatgc caggaacagc agcgctttaa
     4681 agaacgcgtg agtcatcaag tggaaaatcg ccgcatccca tgcctgcacg ccaagcgcga
     4741 ggaacatgta gccaatctgg ctcatggtag agtaagcgag aacacgtttg atgtctgtct
     4801 gtaccagcgc ggcaaaaccg gccagcagca gcgtaaccgc cccgacaata cccaccagat
     4861 gcagaacttc cggggtcatc aggaacaggc cgtgggtacg ggcgatcagg tagacacccg
     4921 cggttaccat cgttgcagcg tggatcagcg cggagacagg cgtcgggcca gccatcgcat
     4981 cggcaagcca tgtctgcaac ggcaactgcg cagatttacc gaccgcaccg cccagcagca
     5041 tcagcgtcgc ccacatcagc atgttattgc cgtcagcaaa gtgcgctggt gccagttcca
     5101 ccatttcgcg gaagttcagg gtgcccagtt cgttgtaaag aatgaacagt gcgaaagcga
     5161 ggaacacgtc acccacacgg gtcacgacga acgctttcat tgccgctgcg ccattcttcg
     5221 gatcggtgta atagaaaccg atcagcagat aggagcacag gcccacgcct tcccagccga
     5281 ggtacatcag cagcaggttg tcggcaagca ccagaactac catgctggcg atgaacaggt
     5341 tggtgtaggc gaagaagcga gagtagccct cttcaccgcg catgtaccag gaggcgtaca
     5401 tgtgaataag gaaacccaca ccagtgacca ccgagagcat ggtcagcgac aggccgtcca
     5461 gcaccagatt gaaaccgatg ttaaagtcgc ctaccgacat ccacgtccac agcggctggc
     5521 tgtatgactg ctcgccgtta gcgaagaaat caacgccgat aaaggcggtt accagcgccg
     5581 ccaggcccac agagccaacg ccgacgatcg ccgaaacgtt ttcagaccag cgcccacggg
     5641 agaacgccag caggacgaag ccaatcaatg gcaaaataat ggttaaggca agcatgttca
     5701 tccgcgcatc tcacttactg aatcgatgtt caggttctgg cgacgacggt gaagttgcag
     5761 cagcagcgca aggccgatac tcgcttctgc cgccgcgagg ctgatggcga gaatgtacat
     5821 cacctgaccg tcggtctgac cccagtagct tccggcgacc acaaaggcca gcgcggaggc
     5881 gttaatcatg atttccagac caatcagcat aaacagcaga ttgcgacgga taaccagacc
     5941 ggttaagcca agaacgaata agattgccgc gaggatcagt ccatgttgta aggggatcat
     6001 gcgtgctcct ccgtttttct tttcgcgctg tcgtctttac gattgctcag cacttcaccc
     6061 gcacgctctt cacgaccgac gtggaaggcc acaaccagac ccgcgagcag cagcatagaa
     6121 gccagttcca ccgccagtac gtaaggcccg aacagcgtaa tacccactgc tttagcactg
     6181 attggcgtac cgtcgatacc ctgatcatta acaccaagga tggcgtaaac aatcaccacc
     6241 agcatgatgg ccgacaaaat tgccggacca atccacacct gcggtttcag ccactggcgt
     6301 tcctgttcga tttctgaacc gcccaggttg agcatcatca ccacgaacac gaacagcacc
     6361 ataatggcac ccgcgtagac gataatttcc agcgcaccgg cgaagtaagc gcccagtgag
     6421 aagaacaccc cggaaatcgc cagcagcgaa ataatcaggt acagcagtgc gtgtaccgga
     6481 ttggtatggg tgatcactcg caaggtcgca agtatggcta tcaggccaca gatataaaaa
     6541 gcgaactcca ttgcccctct ccttacggta acaggctctt gacgtcgata ggcttggctt
     6601 cgttctctgc ttcgccctta tctttgccgt cgattgccat acctgccatc cggtagaagt
     6661 tatattccgg gtatttgccc ggaccggaga tcagcagatc ctctttttcg taaaccagat
     6721 cctggcgctt gtattccccc atttcgaaat ccggggttaa ctgaatcgcc gtggtcggac
     6781 aggcttcttc gcacagacca cagaaaatgc agcgtgagaa gttgatgcgg aaaaattccg
     6841 ggtaccagcg accgtctttg gtttctgctt tttgcagcga gatacagccg accgggcagg
     6901 ctaccgcgca gaggttacag gctacgcaac gctcttcgcc gtccgggtcg cgggtcagaa
     6961 cgatacgacc acgataacgg ggcggcagat agaccggctc ttccgggtac attcgcgttt
     7021 cgcgtttggc gaacgcgtgc aggccgatca tccagatact acgaacctgg gtgccgaaac
     7081 ctactaacaa ttcttttaag gtcatggtct tattgcccct tattgcgcct gccagagaat
     7141 gacagccgcc gttaccagca agttgatcag cgtcagcggc aggcagattt tccagccgaa
     7201 ggacattacc tggtcataac gcggacgcgg taacgacgca cgaatcaaaa tgaacatcat
     7261 cataaagaac gcggttttca gcgcgaacca gatgaatggc ggtaacaacg ggccttgcca
     7321 gccaccgaag aacagcgtca ccatcaatgc agagatggtc acaatcccga tgtactcacc
     7381 cacgaagaac agaccgaact tcataccgga atattcaatg tggtaaccat ccgccagttc
     7441 ctgctcggct tccggctggt caaacgggtg acggtgacat accgccacgc ccgcgatggc
     7501 aaaggtaata aaaccaaaga attgcgggat aacgttccac acatgcgcct ggctgttgac
     7561 gatgtcggtc atgttgaatg aaccggcctg cgccaccacg cccatcaggg aaagcccgag
     7621 gaacacttcg tagctcaggg tctgcgcaga agcacgcatc gcacccagca acgagtattt
     7681 gttgttactt gaccagcccg caaacagcac cgcgtaaacc gccagacctg ccatcatcag
     7741 gaagaacaaa atcccgatgt tcaggtcggc aaccacccaa cccggactga ctggcacaat
     7801 cgcaaaggcc agcagcagcg aggtaaaggc aatcatcggt gccagggtaa agatgacgcg
     7861 atccgagaat ttcgggatcc agtcttcttt aaagaacatt ttgatcatgt ccgcaaccag
     7921 ctggagcgaa ccgccccagc caacacggtt aggtccgtaa cggttctgga acagacccag
     7981 caggcgacgt tcgccaaagc tcatgaatgc cccgcaggtg acaaccacca gcaggatcac
     8041 caccgctttg aggatggtca gcaggatctc aatcagttcc ggtgatatcc aactcattgt
     8101 tgtgcctcct tgagatcctc aagatgcgcg ccagccagca ccggagcaat accggacata
     8161 cccatcggca agcccacctg ccctgccgtc agcccttcag cgatttcaac cggcagcgtg
     8221 accgtgttgc catcgtaact aaaggagacg tgtgtacctg cgttcacacc taacttcgcg
     8281 gcatccgctg ggttgagttt gatgtacggc tgcggcatac ggctctggaa gaccggagca
     8341 cgctgtgaca attcatcgct gccaaacagg tggtaatacg gcgcgatacg ccatttcccg
     8401 tcctgcggct ggaagcgtgc cggtacgctg gtgaagtaat ccagaccatt ttcgctggtt
     8461 tcaaacagac gcacgcccgg atcgccaaag cgcagtttgc cgcccacttc gtcctggaat
     8521 ttgttccacg cctgcgggga gttccagccc ggcgcccagg caaacggcac ttgcgaacgg
     8581 tgcgcagtcg gctggttgtt accttccatc gagaaggtga acatggtgtc aatatcctgc
     8641 ggctgacgcg gctcatgaac gctgatattg gcgcgcatgg cggtacgacc gctgtaacgg
     8701 tgcggttcac gggccagttt ctgcccacga atacggaatg tcgcatccgg cgcagcatct
     8761 ttgatacctg ccagttccgg gattttcgcc acaacagcgt caatcacatg gtcgagctgc
     8821 gtccagtcca cttcacggct cagcagggtg ctgtgcagcg agtgtaacca gcgccagctt
     8881 tccagcatga cagttttgct gtcgtaataa gcaggatcgt aaacctggaa gaaacgttgg
     8941 gcgcggcctt cgttgttgat caccgtaccg tcgctttcag caaagctggc agcagaaagt
     9001 accagatggg cgttttccat aatcgctgtg cgttgatgat caaccaccat caccagcggc
     9061 gctttagcca gcgcagcatt cacgcgggta gcggaagcgt gacgatgcag atcgttttcc
     9121 agcaccacca ccgcgtcggc gcgtccggtt tccagttcgg tcaacgcttc ttcaagcgaa
     9181 ccgccaccca taatgcccag ccccatgctg ttgacggaac gggcaatcat ggtgatacca
     9241 acgtcagcgc cgcgaccttt cagggctttc gcgacgttag ccgcagcctg aatcacctct
     9301 atgctaccgg cgttcgtccc ggagataatc aacggtttct tcgcacctgc cagtgcctgc
     9361 acgatgacgt cgattttgct ttgcagctca ggttcgatac cgtcaaccgc tggcgcagag
     9421 ttatccagcg catgggcgat ggcaaaacct aaacgcgcct gatcttcaac cggtgcgcgg
     9481 taagtccacg ccgcgatatc atccagacgg gtgtcatcaa cgttggtaac aaacagcgga
     9541 tgcttcgcac gttgaccgat gttgaggatt gccgcaatct gccagtcagc cactttctgt
     9601 gctgccgcca tttcgcgcgc tttacctttc acagcctgac gcactgccag cgcgacgcgc
     9661 gcgccggtct gggtaacgtc ttcgcccagc accagtaccg catcgtaaga ttcgatttcg
     9721 cgcagagccg gagtataaat gccgccttca cgcagcactt tcagcgccag ttgcagatgt
     9781 tcctgctcac cgtgagcgat accggtgtag aagttttctt cgcccaccag ttcacgcagc
     9841 gcaaagttgc tttccacgct ggcacgcgga gaaccaatac cgatcacttt cttcgactga
     9901 cgcagaatat ctgccgcgcc ctgcattgcc tgttcggcgt tgagggtaat gaaatcatcg
     9961 ccacgacgct gtactggttg acgcggacga tctttcaggt tgacgtaacc gtaaccgaaa
    10021 cgaccacggt cgcagaggaa gtagtggttt accgtaccgt tgtaacggtt ttcgatacga
    10081 cgcagttcgc cgtaacgttc accggggctg atgttacagc cgatggaaca ttgctggcag
    10141 atgctcggcg caaactgcat atcccattta cggttgtaac gctcggagtg cgttttgtcg
    10201 gtaaatacgc cggtcgggca aatttcgacc aggttaccgg aaaattcgct ttccagcgtg
    10261 ccgtcttccg ggcgaccgaa gtagacgttg tcgtgcgcac cgtaaacgcc cagatccgta
    10321 ccgtcagcgt aatctttgta gtaacgcacg cagcggtagc aggcgatgca gcggttcatt
    10381 tcgtgagaga tgaatggccc caaatcctga ttacggtggg tacgtttggt gaaacggtag
    10441 cgacggaagc tgtgtccggt catcacggtc atatcctgaa gatggcagtt accgccttct
    10501 tcacataccg gacagtcgtg cgggtggttg gtcattaacc actcgaccac actttcacgg
    10561 aactgtttcg cttcttcgtc gtcaatggaa ataaaggtgc catcggaagc cggtgtcata
    10621 caggacatca ccaggcgacc acgcgtgtct tccgcgtttt ggtattgctt caccgcacac
    10681 tggcggcaag caccgacgct tcccagcgcc ggatgccagc aaaagtaagg aatatcaagg
    10741 cccagagaca gacaagcttc cagcaggttg tccgctccgt tgacctcgta ttctttgccg
    10801 tctacatgaa ttgtagccat tagcatgctt ccagttttct cggtcaggga ccgagcgtta
    10861 atcgaaattc ggttaccagc gctctttcag caggttcggc tgaatcccat taatcaaatg
    10921 ggtattgctg aacggctgtt tgattcccgc ctcaaattct tcgcggaaat atttgatggc
    10981 gctctgtaac ggctccactg caccaggtgc gtgggcacag aaagttttac ccgggcctaa
    11041 gaatcgacac agttgctcaa gtgtttcgat atcgcccggc tgaccttcac cacgctccag
    11101 cgcacgcaga attttcacgc tccacggcag accgtcgcgg cacggcgtac accagccgca
    11161 ggactcacgg gcgaaaaact cttccaggtt acgcaccagc gacaccatgt tgatctcatg
    11221 gtcaaccgcc atcgccagcg ccgtacccag acggcttccc gctttaccga tactttcgaa
    11281 ttccatcggc aggtcaaggt gcgcttcggt caggaagtcg gtgcccgcgc cgcctggctg
    11341 ccaggctttg aatttcagac cgtcacgcat accaccggcg taatcttcga ggatctcgcg
    11401 cgcggtagta ccaaacggca gttcccacag tcccggattt ttcacccgac cggagaagcc
    11461 catcagcttg gtgccagcat ctttactttt cgagatgttc tgataccact ccacgccgtt
    11521 agcgaggatc gccggaacgt tacacagggt ttcgacgttg ttgacacagg tcggtttgcc
    11581 ccatacgccg gaggttgccg ggaagggggg tttcgagcgt gggttagcac gacgcccttc
    11641 cagggagttg attaacgctg tttcttcccc gcagatgtag cgccctgccc cggtatggac
    11701 gaacagttcg aagtcgaaac ctgttcccat aatgtttttg ccaagcaagc ccgcttcagt
    11761 ggcttcggca atggcgcggc gcagatttac tgccgcttcg atatattcgc cacgcaggaa
    11821 gatgtagcca cggtaagctt tcagcgcaaa cgcggagatg agcatacctt ccaccagcag
    11881 gtgcggcagt tgctccatca gcaggcggtc tttataggtg cctggctcca tttcatcggc
    11941 gttacacagc aggtaacgga tgttcatgga ttcgtctttc ggcatcaggc tccatttcag
    12001 gccggtagaa aagcccgcgc caccgcgccc tttcagacca gcgtctttta cctgattaac
    12061 gatttcgtcc ggagacagcc cggtcagcgc cttacgcgcg ccttcgtaac cgtttttgct
    12121 gcgatattcg tccagccaca ctggctgttt gtcatcgcgc agacgccagg tcagcggatg
    12181 cgtttcggga gtacggataa tgtttttcat ttataccgct ccagcagttc agggatcgct
    12241 tccggggtca gatgcgcgtg agtatcctca tcgatcatca tgtttggccc tttatcacag
    12301 ttccccaggc agcaagttgg aagcagcgta aagcgaccgt caaatgtcgt ttgccctggt
    12361 ttgatgttta gctttttctc gagcgccgcc tgaataccct ggtaaccgtt gatatgacag
    12421 accacgctgt cgcaataacg gatcacatgg cggccaaccg gctggcggaa gatctggctg
    12481 tagaacgttg ccacaccttc gacgtcgctt gccggaatac ccagcacatc ggcgatcgcg
    12541 tggatcgcac catccggcac ccagccacgc tgcttctgaa cgattttcag cgcttcaatg
    12601 gacgccgcac gcgggtcttc gtagtggtgc atctcgtgct caatcgcttc acgctctgcc
    12661 gcactcagct caaaagcctc ggtttgtggt tgttgattct cgtgcataat tagcggtcca
    12721 catctgacat aacaaaatcg atactgccca gataaacaat caggtcagac accaggctgc
    12781 cgcggatcgc cgccggaatt tgctgcaaat gcgcataact cggcgtgcgg atacgagtgc
    12841 gataactcat ggtgctaccg tcgctggtca ggtagtaact gttgatcccc ttggtcgcct
    12901 caatcatctg gaaagattca ttggcaggca tcaccggccc ccacgacact tgcaggaagt
    12961 gggtgatcag ggtttcgata tgttgcagcg tgcgctcttt cggcggcggc gtggtcagcg
    13021 ggtgatccgc tttgaacggg ccttccggca tgttgttgag gcactgctca agaatgcgca
    13081 gactctggcg cagctcttcc actttcagca ttacgcgggt gtagcagtca gaaacaccac
    13141 caccaaccgg gatttcaaag tcgaagtttt catagccaga ataaggacgc gccttacgca
    13201 cgtcgaagtc gatcccggta gcacgcaggc ccgcgccagt ggtgccccac tccagcgcct
    13261 ctttcgcgcc ataggcggca acgccctggg aacgaccttt cagaatggta ttttgcagcg
    13321 ccgctttctc gtaagacgcc agacgtttcg gcatccagtc gaggaactca cgcagcaggc
    13381 gatcccagcc gcgcggcagg tcgtgcgcta cgccgccaat acggaaccac gccgggtgca
    13441 tacggaaacc cgtgatcgct tccaccagat cgtaaatttt ctgacgatcg gtaaaggcga
    13501 agaacacggg cgtcattgcg ccgacgtcct gaataaaggt cgagatgtac agcaggtggc
    13561 tgttgatacg gaacagttcg gagagcataa cgcgaatgac gttaacgcga tccggcacgg
    13621 tgatcccggc cagtttctct accgccagca cgtaaggcat ttcgttaacg cagccgccga
    13681 ggtattcaat acggtcggta tacggaatgt agctgtgcca ggactggcgc tcgcccattt
    13741 tctccgcacc acggtggtgg tagccgatgt ctggtacgca gtcgacaatc tcttcgccgt
    13801 cgagttgtaa aacgatacgg aaagccccgt gcgccgacgg gtggttcgga ccgaggttga
    13861 ggaacatgaa gtcctcgttt tcggtgccgc gcttcatccc ccactcttcc ggtttgaagg
    13921 tcagggcttc catctccaga tcctgtttgg ctttggtcag ctcaaacggc gagaattcgg
    13981 tagcgcgcgc cgggtaatct ttacgcagcg ggtgaccttt ccaggtttgc ggcatcatga
    14041 tgcggcgcag gttcgggtga ccgtcgaaag taatgccaaa cagatcccag gtttcacgct
    14101 cataccagtt agcgttcggg aacagtttgg tgaaggtcgg tacgtgcagg tcgttttctg
    14161 ccagcgccac cttcagcatg atgtcgcggt tacgatcgat agaaatcaga tggtagaaaa
    14221 cggaaaaatc cgcggcaggt aacccttcgc ggtgtgtgcg cagacgttcg tccatgccgt
    14281 gtaagtcaaa cagcatgacg taaggtttcg gcagtttctt taagaaatcg ccaacttcca
    14341 gtaattgttc acgcttgatc cacacaacgg gaaccccggt gcgagtcgcc tgaacagtaa
    14401 aggcatccgg cccaaaacgg ttgcgcagtt cgccaatcac cggatcatca agatgatcgc
    14461 gggtctgcca ggcgggttct tgcgcggtta agtcggtcat attgttcacc attgcaaatg
    14521 gtccgtggtg actgtcggcg gagtaagctt cgcgaaatag atttaggaat aagcgaagaa
    14581 aaatcccttt gccgacaggc gcaaattaaa tctcgtcagg tgtacgcagg ttggttacgg
    14641 caatgcgttc accgcgcttg cgttcgcgct ctgattgcat attggcgcga taaacgccct
    14701 gatcgccaac cacccaggag agcggacgac gttctttgcc gatagattcc tgcaacagca
    14761 tcagtgcctg catgtacgct tcaggacgcg gcgggcagcc cgggatatac acatcaaccg
    14821 ggatgaattt atcgacgccc tgcacaacgg aataaatatc gtacataccg ccggagttgg
    14881 cacaggcacc cattgagata acccattttg gttccagcat ctggtcatac agacgctgaa
    14941 taaccggtgc cattttggta aagcaggttc ctgcaaccac catcaggtca gcctgacgcg
    15001 gcgaagcacg caatacttct gcgccaaaac gcgccacgtc atgcaccgcg gtaaacgaag
    15061 tcaccatctc aacgtaacag caggaaagac cgaagttata cggccaaatt gagtttttac
    15121 gaccccagtt aaccatgtca ttgagcttgc ccataaacac gtttttgtta acttcttgct
    15181 ccagagggtc ggttacgatc tcctgctttt gcagggggta acggtcgttc tcaccgttgg
    15241 gatctatgcg ggtgagcgta taatccatct taatgcctcg cggttagcgt tgacgattag
    15301 cgatactgtt cgtttccggg ttcatacgct cgcggcgtga acgcgcgggc gtccagtcca
    15361 gcgcgccaat acgcaccaga taaaccagac ctgccagtaa cacaaaaata aaaattgcag
    15421 cttccacaaa gcctacccag ccgctttcgc ggatagaggt tgaccatgcg aacagataca
    15481 gcgcttcaac gtcgaagata acgaagaaca tggccaccag ataaaacttg gcggacaggc
    15541 gtaagcgggc ggagccgacc gagtcgatac cggattcaaa cggcacgttt ttcgacctcg
    15601 cgcgtgcgcg accgcctaaa aaccaaccgc ctaccagcat caggcaacac aggccaatgg
    15661 caacgataag aaagatagcg aatgcccagt gatgagcgat gacttcagtg gatgttgaca
    15721 tactcattgc ttactcatca aaagtagcgc cagattcact gctcttcacg gcagatggac
    15781 gccacatcga ttcatgggga ggaataaaaa aaaccttaca atcactgtag aaattctttt
    15841 atacagctaa ttgatgtggt cttttactcc tttctataac cttttgtcaa ctttaacaaa
    15901 agtttcttca cattagttta cataatatca acaccattag catttaatgc cctttcaccc
    15961 cagatccttg acgactccag gataattaga tgttgttgaa tcgtgtccgt tgtgaagcaa
    16021 tggaaaaaat acgggtctat tttgacagga atttgtgtcg attcctcccc caaaagagag
    16081 tattttcttg atctgtgaca cgcttttgtc attccataac aaaaacgcag caacaaattt
    16141 acgtattttt taacatcatt gtagcaggtg atttttttca ggcgattatt tgtgcgttcg
    16201 ggacgtgaat ctctggtgat tgaaaaatga acagttttgt acgttctgca ctatgcgagg
    16261 aaggattttt actaaaaaaa agccgctggg gtttaaaaca cccccagcgg ctcgtttttt
    16321 acactattgt ctcaggaatt atctatcgtc cgtcgattac tcgatatccc tttcaatcaa
    16381 caatgaatca tccccttccg gagcagacat tgggctgtat tgccacggat tatggtagct
    16441 ctccatcgcc tgatagatca cctgggccag ctcattatta ctggacgggt catagcacag
    16501 caaatattcg gtatcaggta gcggcggtaa gccatctact ccactcagca cacgcaggtc
    16561 cgggctcatc atctcaaccg gccttgccgt cacgccaaga ccggctttca ctgccgcacg
    16621 aacggccgga agcgtcgagg cgacataagc cagtcgccat ggaatatctg ctttattaag
    16681 cgtcgccagc accatatcgc gaaacggact tggatcatcc agcaatacaa gagggatcgg
    16741 ctcgcctttt tgcaatacgt attccgctgc gcagtaccag tgtgttggcg aggtacgcag
    16801 gttcaatgcc ttaaacgctg aaggacgatg ggtggttacc atcaaatcca cttcctggga
    16861 ttccagcatt tctgccatat aggcattacg tttaacgcgg acatccagcg ctaatttcgg
    16921 ataaaccgaa ctcacgcgat ttaacaggaa aggtaagatc gtatcggcag attcatctga
    16981 agcaccgata gttaacacgc cctgaagatt actgaacatt aatgatgagc aggcctcatc
    17041 attaaaacgc aggattttcc tggcgtaacc aagaagttga atgccatgtt cagttaacag
    17101 tttgttgcga ccgtgacgag cgaacagttc tttcccaacg agttgttcca gacgctgcat
    17161 ttgctgactt acggcggact gagtacgaca cacagcggca gctgcggcag caaaagtgtt
    17221 cagatcggca acagcaacaa atgttctcag cagatcgagg tcgaggttaa ttatcggacg
    17281 atttgcactt atcatatatt atcacttact ggcggctcat actgagctgg ttaatgctgt
    17341 gcacacacaa acaagcaatt ccatttgtaa tgtgcctccc tggcagtttc atccggaacc
    17401 cggacgaaag taaaaatgca tatgagttgc actaaaaaag cgactcacat tgttccgtta
    17461 taatgcctga agtagatcac agaatatatc ttcagggatc gcatatctat taagttactc
    17521 actcttttct atttatgaca tgcgcgtgtt tgtataaatg taaatgtgag tccttgttcc
    17581 actctcgtgc agcatcgctg gtcatacgcg aacacgtaca aacagcaatg gtgaggcggc
    17641 atcaagagca gggatccgtc atttatgcga gcattttacc ccaaaaacct tttatttata
    17701 agggtcattg cgaattatct gatgcaaagt tatgttatgt taggcaaagt aatcttctat
    17761 tattaataag cacatcaaaa cctttttgaa ta
//
LOCUS       DAJOWB010000096        17653 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJOWB010000096 DAJOWB010000000
VERSION     DAJOWB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.096"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            114..266
                     /locus_tag="OHO85_003483"
     CDS             114..266
                     /locus_tag="OHO85_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCQ2672813.1"
                     /translation="MAAPVLATYISGYYGLGVSLFIVTVAFSALLILLVGFDIPGKIY
                     KLSVAK"
     gene            278..1396
                     /locus_tag="OHO85_003484"
     CDS             278..1396
                     /locus_tag="OHO85_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCQ2672814.1"
                     /translation="MINYGVVGVGYFGAELARFMNMHDNAKITCVYDPENGENIAREL
                     QCINMSSLDALVSSKLVDCVIVATPNYLHKEPVIKAAKNKKHVFCEKPIALSYEDCVD
                     MVKACKEAGVTFMAGHIMNFFNGVQYARKLIKEGVIGEILSCHTKRNGWENKQERLSW
                     KKMKEQSGGHLYHHIHELDCVQHLLGEIPETVTMIGGNLAHSGPGFGNEDDMLFMTLE
                     FPSGKLATLEWGSAFNWPEHYVIINGTKGSIKIDMQETAGSLRIGGQTKHFLVHETQE
                     EDDDRRKGNMTSEMDGAIAYGHPGKKTPLWLASLIRKETLFLHNILCGAKPEEDYIDL
                     LNGEAAMSAIATADAATLSRSQDRKVKISEIIKHTSVM"
     gene            1439..1564
                     /locus_tag="OHO85_003485"
     CDS             1439..1564
                     /locus_tag="OHO85_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672815.1"
                     /translation="MIKNFIFDNLIILAVPFMIKTSLKTNLIFFFLCVFVPHMAS"
     gene            complement(1617..1874)
                     /locus_tag="OHO85_003486"
     CDS             complement(1617..1874)
                     /locus_tag="OHO85_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672816.1"
                     /translation="MVSSSASNVVNCETKQRTQFECIYFSQYWAKGDFIAKRAPIGQW
                     EPYSEESLLGIIVTSVCRIKVAMLKPEPPRDPHIPLMGDFN"
     gene            1864..2100
                     /locus_tag="OHO85_003487"
                     /pseudo
     CDS             1864..2100
                     /locus_tag="OHO85_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939276.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            2188..3354
                     /locus_tag="OHO85_003488"
                     /pseudo
     CDS             2188..3354
                     /locus_tag="OHO85_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3437..3694
                     /locus_tag="OHO85_003489"
     CDS             3437..3694
                     /locus_tag="OHO85_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672817.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(4251..5018)
                     /gene="fecE"
                     /locus_tag="OHO85_003490"
     CDS             complement(4251..5018)
                     /gene="fecE"
                     /locus_tag="OHO85_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCQ2672818.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(5019..5975)
                     /gene="fecD"
                     /locus_tag="OHO85_003491"
     CDS             complement(5019..5975)
                     /gene="fecD"
                     /locus_tag="OHO85_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCQ2672819.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(5972..6970)
                     /gene="fecC"
                     /locus_tag="OHO85_003492"
     CDS             complement(5972..6970)
                     /gene="fecC"
                     /locus_tag="OHO85_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCQ2672820.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(6967..7869)
                     /gene="fecB"
                     /locus_tag="OHO85_003493"
     CDS             complement(6967..7869)
                     /gene="fecB"
                     /locus_tag="OHO85_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCQ2672821.1"
                     /translation="MLAFIRFVFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEIVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(7914..10238)
                     /gene="fecA"
                     /locus_tag="OHO85_003494"
     CDS             complement(7914..10238)
                     /gene="fecA"
                     /locus_tag="OHO85_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCQ2672822.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLTSRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(10325..11278)
                     /gene="fecR"
                     /locus_tag="OHO85_003495"
     CDS             complement(10325..11278)
                     /gene="fecR"
                     /locus_tag="OHO85_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCQ2672823.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(11275..11796)
                     /gene="fecI"
                     /locus_tag="OHO85_003496"
     CDS             complement(11275..11796)
                     /gene="fecI"
                     /locus_tag="OHO85_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCQ2672824.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            12088..12785
                     /locus_tag="OHO85_003497"
                     /pseudo
     CDS             12088..12785
                     /locus_tag="OHO85_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(12805..12870)
                     /locus_tag="OHO85_003498"
                     /pseudo
     CDS             complement(12805..12870)
                     /locus_tag="OHO85_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
     gene            13103..13207
                     /locus_tag="OHO85_003499"
                     /pseudo
     CDS             13103..13207
                     /locus_tag="OHO85_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(14141..14272)
                     /locus_tag="OHO85_003500"
                     /pseudo
     CDS             complement(14141..14272)
                     /locus_tag="OHO85_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            14554..15912
                     /locus_tag="OHO85_003501"
     CDS             14554..15912
                     /locus_tag="OHO85_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCQ2672825.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(16151..17536)
                     /locus_tag="OHO85_003502"
     CDS             complement(16151..17536)
                     /locus_tag="OHO85_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCQ2672826.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLESMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVPGVIADWPVNRVSE
                     LLPWRIALPAE"
BASE COUNT         4299 a         4481 c         4723 g         4150 t
ORIGIN      
        1 ttacaaattt aggtattgca gggttggttc caaaatttat atatgattac tttccaacaa
       61 aattaagagg attagggacc ggtcttattt ataacttagg ggcaactgga ggaatggccg
      121 cacctgtatt agctacatac atttcaggat attatggctt aggtgtttca ttattcattg
      181 ttacggttgc attctctgcc ttattaattt tgttagttgg ttttgatatt ccaggtaaaa
      241 tttataaact atccgtggct aaatgattgg aggctttatg attaattatg gcgttgttgg
      301 tgttggatac tttggcgctg aattagctcg ttttatgaat atgcatgata atgcaaaaat
      361 tacatgtgta tacgatcctg aaaatggaga aaatattgcc cgtgaattgc agtgtatcaa
      421 tatgtcaagc ttggatgctt tagtctcaag taaattagtc gattgcgtga tcgtagccac
      481 cccaaattat ctgcataaag aaccagtaat taaagcagca aagaataaga agcatgtttt
      541 ttgtgaaaaa ccaattgcat taagttatga agattgtgtg gatatggtca aagcgtgtaa
      601 agaagctggt gtgaccttta tggccgggca tattatgaat tttttcaatg gggttcaata
      661 tgcacggaag ttaattaaag aaggtgttat cggcgaaata ttatcatgtc atactaagag
      721 aaatggctgg gaaaacaaac aagagagact ttcctggaaa aagatgaaag aacaatctgg
      781 tggacatcta tatcatcata tacatgagtt agattgtgtt cagcatttac ttggagaaat
      841 accagagacg gttactatga ttggtggaaa tttggcccat tctggtccag gatttggcaa
      901 tgaagatgat atgttattta tgaccttgga attcccgtca ggaaaactag caaccttaga
      961 gtgggggagt gcatttaact ggccggaaca ttatgtcatc atcaatggaa ctaaaggctc
     1021 tattaaaatt gatatgcaag aaacagcagg gtcacttagg attggcggtc agacaaagca
     1081 ttttttggtc catgaaacac aagaagaaga tgatgatcgt cggaaaggca atatgacctc
     1141 agaaatggat ggcgctatag catatggtca tccaggaaaa aaaacaccat tatggcttgc
     1201 cagtttaata agaaaggaga cgttattcct ccataatatc ctctgtggtg caaaacctga
     1261 agaagattat attgaccttc tcaatggtga ggcggccatg tcggcgattg ctactgctga
     1321 tgctgccact ctttcaagat cgcaggacag gaaagtgaaa atcagtgaga tcattaaaca
     1381 tacatcagta atgtaaaaac tatacgcctc ttgatttaat tcaggaggcc ttttatgtat
     1441 gatcaagaat tttatcttcg ataatctcat aattttagca gtgccattta tgataaaaac
     1501 atcccttaaa accaatctaa tctttttctt cctctgtgta tttgttcccc atatggcgtc
     1561 ataatttacg ctacgtaata cgggatagtt acgatacgca gcgatagcgc taagttttag
     1621 ttaaaatccc ccatcagcgg gatatgggga tctctgggag gttcaggttt tagcatcgcg
     1681 actttaatac gacagacgct ggtgacgatg atgccaagta gtgactcttc cgaataaggt
     1741 tcccactgac caattggcgc gcgcttcgca ataaaatctc ccttcgccca gtattgggaa
     1801 aagtagatac attcaaactg tgtacgctgt ttcgtctcac agttcacgac attgctggcc
     1861 gatgagctga ccattgggcc tatccgggct gtcccgatgg atattacgcc gaagtatgtg
     1921 ggaattgcca gcggattgat gaacgccggt tccgctgtcg ccgacattat ttcacctatc
     1981 gcctttggca ttattatcga caaaacaggc aactggagtt taccattcta cggttccgtc
     2041 gcattgcttg ttattggtat attcctgacg ttcttcatgc gtccagataa gtcattgtaa
     2101 aagtcgaatc agggctgaag tggcacactg aatttggcca cctgaacaga ggtgatatgc
     2161 tcacctcaga acaacacagg tgctccaatg aaaaaaagaa atttcagcgc agagtttaaa
     2221 cgcgaatccg ctcaactggt tgttgaccag aaatacacgg tggcagatgc cgcgaaagct
     2281 atggatgttg gcctttccac aatgacaaga tgggtcaaac aactgcgtga tgagcgtcag
     2341 ggcaaaacac caaaagcctc tccgataaca ccagaacaaa tcgaaatacg taagctgagg
     2401 aaaaagctac aacgcattga aatggagaat gaaatattaa aaaggctacc gcgctcttga
     2461 tgtcagactc cctgaacagt tctcgataat cgggaaactc agagcgcatt atcctgtggt
     2521 cacactctgc catgtgttcg gggttcatcg cagcagctac agatactgga aaaaccgtcc
     2581 tgaaaaacca gacggcagac gggctgtatt acgcagtcag gtacttgagc tacatggcat
     2641 cagccacggt tcggccggag caagaagcat cgccacaatg gcaacccgga gaggctacca
     2701 gatgggacgc tggcttgctg gcaggctcat gaaagagctg gggctggtca gctgtcagca
     2761 gccgactcac cggtataaac gtggtggtca tgaacatgtt gctatcccta actaccttga
     2821 aaggcagttc gccgtgaccg agccaaatca ggtgtggtgc ggtgatgtga cctatatctg
     2881 gacgggtaag cgctgggcgt acctcgccgt tgttctcgac ctgttcgcaa gaaaaccagt
     2941 gggctgggcc atgtcgttct cgccggacag caggcttacc atgaaagcac tggaaatggc
     3001 atgggaaacc cgtggtaagc ccgtcggggt gatgttccaa gcgatcaagg cagtcattat
     3061 acgagcaggc agttccggca gttactgtgg cgataccgga tcaggcagag tatgagtcgg
     3121 cgtggaaact gctgggataa cagcccaatg gagcgcttct tcaggagtct gaagaacgaa
     3181 tgggtgccag cgacgggcta tgtaagcttc agcgatgcag ctcacgcaat aacggactat
     3241 atcgttggat attacagcgc actaagaccg cacgaatata atggtgggtt accaccaaac
     3301 gaatcagaaa accgatactg gaaaaactct aacgcggagg ccagttttag ttgaccacaa
     3361 cagactacct gaagggagcc gcggtcgcct ggcagttgca gtagcaggag atcatccagc
     3421 cgcagtacag gtcacgatga ctctggttaa tgataccggc tttgaccccg tattttccgg
     3481 ctctatcgct gaatcatggc gtcagcagcc gtgcacacca tcctattgtt gtgactggga
     3541 ggctgccacc atgcttcgcg ctttccctct ggcgaaaaag ggagaaggac gggcccgtct
     3601 gccttcactt tatgccagct tcggtaagct gggtgagaca ccgactcatg aagatatcat
     3661 tgataacaat cgatccatca actggcctgt ataacgtggc tgccggtgat taagaaagct
     3721 gcacctacct aagtagtagc aaacgcacac tttttagaaa aatcgatggt cagaaactgg
     3781 attagcaatt ccgttccagg gttgcttttg atttacgttg gcgtctgatc attgatttat
     3841 cctcaaaagc ccaacctcat tggtaatgaa ccagctccgt gaatgtccgc tctggcacag
     3901 agcgaaattt tttgatctcc cccctgaaat ctaaacttag tcatgtcacg tttttgggtt
     3961 tctaaaattt taacttcgcg tttttcgttg ccgtaagggt tatacagaaa tgtccgttaa
     4021 gcagagttca aaattgattg ccgtgatcac gactggtttg aaagccgcgc ccaagcctgt
     4081 acagctctgg tttgcgttga ttatgaacct gtcagcctaa agcaagcgga tggacgatga
     4141 gtattggtaa tctttcagag tccggaaaag ttcagcccca gtctgaacag gcttgctggc
     4201 gccagtccag tttcattcag tcgtggtttg gttcttacgg cctgtgcaat ctacctcatt
     4261 aggcacatcg gcctgccaga taccggctcg gggtgtattt ccgcttccac gctgaatact
     4321 gttctcagca atcctggggt catcacctct tctggtgtgc cttgcgccat aacatgtccg
     4381 tttgccatta ccaccagttg atcgcagtac cggctagcct gattaaggtc gtgcagcaca
     4441 gcgaccaccg ttttcccctg agtccggagt tcgcccatca accgcatcag gtccacctgg
     4501 tgattgatat caagataggt ggttggctca tcaagtaata caacgggcgt attctgggcc
     4561 aggaccattg ccagaaatgc gcgctggcgc tgaccgccgg aaagctcggt taaccgacga
     4621 acggcaagat gattgatccg ggtctggttc atggcgacat taactcgtgc attgtcttca
     4681 gcggagagac gcccccagag tgacagccag ggattacgac catacgaaac cagctcctgg
     4741 actgtgatcc cctctggcgt taaatggtgc tgaggcagca gcgaaagcct gcgggccaac
     4801 tggcgcgatg agagcatatt tatgggatta tcgccgagaa atacggtgcc agactgcggc
     4861 attaaaagcc gcgaaaaaca gtttaacagc gtcgatttcc cgcaaccgtt aggaccgatc
     4921 agggcggtga tcttccccgt tggcagtgag agtgaaacgt cgttaagtac cttgtctgtc
     4981 ccgtaactga ccgtcagatt ttcagttcgt aaagtcattt atcgcattct cacaagcaac
     5041 cagacaaacc acggcgcacc gataatggcg gtcagcacgc caaccgggag ctccagtggg
     5101 ggatgaataa ttctcgccag cagatcggca accaccaaca gcaacgcacc tgtcagggcc
     5161 gaaacaggca gcagtctgcg gtgacgtcca ccggtgatgc tacgcatcat atgcggcacc
     5221 acgagaccaa taaagctaat cgggccgcag gcggccacgc cggtagatgt catggcgaca
     5281 gctagtaaca aagcccagaa tcgggtatgg ggcaccgaca caccgagcgt ggtggcgcgc
     5341 gcatcgccga gtgcaaggag gtcgagatcg cggcaaaaac tcaggctcag cggcagaaat
     5401 aaaatcatca gcgggatggc aatcttcaca aagctccagt cacggcccca taagctgccg
     5461 gtcagccaca gcagggcgtt gttcacatcc tgcgggcgcg agagcatcag ataatccgtc
     5521 aggctggccc agcatgcaga aagcgccacg ccggtgagcg ccagcttcat cggctggtgg
     5581 gtctttgcca gcatcttcag caatatcaac cccgccatgc cgcccgcaaa ggccagcagc
     5641 ggcagcacca tcacgggcag tgacggcata agaagtagag cccccacaga ggccaggctg
     5701 gcggcatggt taacaccgag aatatccggt gatgccagag ggttgcgcac aatcccctgt
     5761 atcagcacgc ccgccacggc gagggctgca ccgacaaaca gtgccagcag caagcgcggc
     5821 agtcggtact ccatcaatac ataataatgc tcgcgtccgg cctgccagtc ggtcagcagc
     5881 gcgcgccacg gcacggggat cactcccata tggagtgata acagcgcaca gcccgccagg
     5941 gcaagggtga tgaaaataac cagcgcaatt ttcatcctcg cctcctcaca agccagacaa
     6001 agcaagggct gccaatcagc gccagcactg cgcctgcggg cagatctccg gggaaggcca
     6061 gtgcgcgtgc gagtacatct gccagcagca tcagcgtggc ccccagcagc atgctcactg
     6121 gcagtacgtt gcgctgatcg aagcctgccc agaagcgcgc cagatgtggc accagcagac
     6181 cgataaacgc caccggacct gccacgctga cgcacgcacc aaccagaagc agcactaaca
     6241 tattgatgac caaacgtagc ctcgtcaggt tcactcccag cgtatgggcg gtgctgtcgc
     6301 tgaggttgag caggttcagt tgattcgcca gcagcaacac gacagggact gcagtgacca
     6361 ccaccggcaa gagctgccag acatcctgcc agcgggcgtg ggacactccg cctgccagcc
     6421 agtaaaagat gccgtaagca tgatcttcgg ccagcagcag ggtgatgcgg gtaaggccca
     6481 tacaaaaggc cgacagcgcg atacccgcga ggatcagttt gtttctgtca tgggtatgac
     6541 gaaatccgcc tcctgcggtc atgaccagca gccagctcac gccgcccccg catgccgcga
     6601 tgaacgacag agaatagcct gcaatcggcg tcggactcag cgcgctggta agcgccatag
     6661 ccagcgccgc gccgctgtta atgccgagca gtgaaggaga ggccattggg ttgtgggtca
     6721 gggtttgcag cagcgtgccc gcgagcgcca ggcttgcgcc gatcagaacg gcgaccaggc
     6781 ttcgtggcaa acgaaggttt tgcaccagcg cttctggtag cgttggcgtg tgtccaggca
     6841 gcagggcgcg ggttgcatct gctccggaaa caggaatggc cgagtagcaa aacagactca
     6901 gccagaaaat aataataagt gctgcaacgg gaagccccca cagcagcacc gggtgtttta
     6961 tcgcggtcat ttcacaacgg taagcggctg atggtggaag atttttaccg tgtcagcggc
     7021 aatacgctct gcagcaaaaa taccgcgcat ccgcgcccag gtgttactgt cgaccgaagc
     7081 aacctgctgc ttctgcgcgg cggttaacat ctgccagagc ggatcttgtt gccagcgttt
     7141 aacaatgctc tcttcgcgat agtgggcaac cagcagccag gcaggattga ccgccagcag
     7201 ttgctccagg ccgatggacg gcatggacgc acccgccatc gcagcgggaa cgttcagccc
     7261 cagagaggcc agcacgctgc cggtccaggt ctcctgagta tgcaggttga attgctgttc
     7321 gcgtgatgtg ccaaaggcca cgcgtgtccc tttgggaagc tggctggccc actgcgccat
     7381 cctctcttta tgttgttcca ggcgtgcctg catctctcgc tttttaccca ctatttcgcc
     7441 gatgatagcc gcagattgca aattttcagc gtaggtttcg ttgcgggact taagcagcag
     7501 taccggcgcg atttgctgca aggcgatgta aacccccgca tggcgactgc tgtcggcaat
     7561 gatcaggtct ggtttcagag cggcaatggc ttccaggctc ggctgcgcgc gcgttccgac
     7621 ggactgccac ggtttcaggt gcgcacgcac ttcgggcagg atgcgttttg catcgttatc
     7681 gtcggcaata ccgatcgggc tgacgtccac ggcggccagc gcatcggcga acgagagttc
     7741 cagcaccaca atccgttgtg gcgttttttc gagtgtaaac gtgccgtgtt cgtcctgaac
     7801 cgtggcggca aaggcgtggc tgatcaccag cagcaggcct gcaaaaacaa aacggataaa
     7861 tgccaacata atcacattcc agctaaaagc ccggcaagcc gggcgttaac acatcagaac
     7921 ttcaacgacc cctgcatata cagcgtgcgc ggctggcctg catagatgcc tttgttgttg
     7981 tcgtcataag agcggatgaa gtagtcctgg tcgaagatgt ttttcacacc gaatgccagg
     8041 ttcagatctg ccatctgcgg gccaaagtca tacgccacgc gtgcgcccca gagcatgaag
     8101 ccgggaatgc ggccggtact gccgtcggcg ctctctttca ccgtattggc gttatccgca
     8161 aactggctgg actggaaatc gctgttcaga ttgaacgtcc agtttcctgg cttgtagtcc
     8221 acgcccagcg tgcctttatg tttcggggag aatggtacca gattgccgta ggtgtcgcct
     8281 ttctcgcgga tttccgcgtt cacatacgca tagctggcgt agatggaaac gttatcaagc
     8341 gttggcgtta gcgtacccag atcgtaacgt gcctgcgttt ccagcccggt atggcgcgtt
     8401 ttgccacgtg cagtgacggt gtcgttggtc tggttggagt cgtactgatt gttaaagtta
     8461 atcaggaaca gccccatttc cgccgtcagc gcgccgtcgt cgtagcgggt accgagttcc
     8521 caggttcgcg ctttttccgg ttccacattg ccgctttgca cagccttgcc aatctggctg
     8581 tactgtacgg tgccgaacga gccttcagtg tttgcataaa gattccagct gtcagtcagg
     8641 tgatagagca cgttcaacgc cggaagcggt gcgttatagc tcacttcttc gtgcgtgcct
     8701 gtgatggcgt tgttctggta tgactcgata tgttcgaaac gcatacccgg cgtgatggtc
     8761 cagttgccga tgtcgatttt gtcatccaga taccaggcgt gcgcctcggt gccggaacgc
     8821 gtatcgcggt cgtaagggct tgagccggac ggcaactgcc cgctgctggt ggcggtgtag
     8881 taacgcattt catgcgttga ttcattcaca tagcgatagc ccacgcccac ttcgtgcgcg
     8941 gaagggccga tcataaagat ctggctgtag cgtggctcaa taccgcgcac ccagtagtta
     9001 cgcggcgaga gggtgatgcg tttgccttgc tccaggtagc cgctgcgcag ggtttgggtg
     9061 tagaacccct gaatgttgaa tttatgctgg ctgtctggct ggaactggta gcccaggctc
     9121 gccagcttgc gacgacccca gaagcggtca tacgggcggg tggattgcca gcgatcggcg
     9181 tcgtaatccg cgcgagacag gccaccgggc atgtcggctt caccgtcgta atattgcagc
     9241 aggctgttga aggtgtgcac ctcatccggc gcatatttgc ttttcagcat caggtcgtcg
     9301 atgcgggtgg cgctgtgctc gcgccagtca ctgccgcgcg tgccggagta gagcagcgcg
     9361 gtgccaaaac cgttgtccgc tgtgccgccc accatcaggt tgtgcgtctc tttcgggttg
     9421 ttttgtgaag aggttgggct gagctgacct tccacgcccg cctcgatacc aaagtcctgc
     9481 ggaatggcac gggtaacaaa gttcaccacg ccgcccacgc tctgcggtcc gtaacgcacc
     9541 gcaccaccac cgcgtaccac gtcaatggca tccatgttgc cgagcgaaac gggagccagt
     9601 gaaagctgcg gctgaccgta aggggcgaag gggacgggga tgccgtccat caggacggtc
     9661 gagcggctgg tgaggcgcgg gttcaggccc cggatgccaa agttcatcgc caggtcgtgg
     9721 ctgccggtgc cgttgttttc cggcgcgctg acgccaggga tgcggttaag tacctcacgc
     9781 atggtggttg cgccggtttt ggcgaaatcc tcacggcgga tcacgtcacg cgcgccagca
     9841 tgttcaaata cgtcgttttc acgcgcatca cccagccagt cgccgaccac ggtcagggca
     9901 tcttcttttg gtgcgggcgc gggctccagc gtccagctat tatttcccag cggttttacc
     9961 tgcagtccgc tgccgtccag cagttgttgc aggccgctct cgacgtcgta atcgccgtgc
    10021 aggccgttgc tctgcttgcc gcgcgtcagg ctggcgtcaa ccgagagggt aaatccgctg
    10081 tgtgcggcat actgattgag cgctttatcg agcgatcccg gtgcgatatt aacctgtgca
    10141 gcaaaagcgg aaaacgagag accggccagc ggcagcaggc tcaggcgaat ggcgttaacc
    10201 aaaggtgttg ttttacgaaa aacgcgtaac ggcgtcatac cttccccatc atcatttttg
    10261 ttgtgttcag ctatgagtcg aacgagaagg gtaaaaagga caatcgaaat aagaattatt
    10321 ttccttacag tggtgaaatg tttatccagt accgcgtaat agactgaatt ttaacgggaa
    10381 gcgtttgcgc gataacgttc aggatcgcat cggtattttt cagcgggaac gtcccgctca
    10441 ggcgcagccc ggcaacggcg ggatcgcagc gcagcacgcc gttgcggtaa cgggttagcg
    10501 tggctatcac ctcacccagt ggtttatcgc tgaagctcag gatgtccttc gtccagcttg
    10561 tactctcgtc atccagcggt ttcactgcgc caaactcaga ggcgctgaac tgcaggcttt
    10621 caccagcgtt cacgatgcgt ttttgcgcgg gggcactggc gagaagcact tccacagcgt
    10681 gctgctgaac gtcaagctgc gtgaaattat cctgctggcg gacggtaaat tctgtcccta
    10741 aagcagtgag ctggccctga cgggtcagga cacggaaggg gcgttgcagg gcatctttcg
    10801 cggtagtaat ggcgatttca ccgtaccaga gccggacggt gcgctgatgc gcatcaaaac
    10861 gcacatccgc cgcgctttgg gtattcagcg tgagcaggga gccatcttcc agttgctgac
    10921 ggctgactgt gcctttggcg gtgcggtaat ctgcccgcag accttcgccg gtttccgact
    10981 gccagagctg ccagcctccg ccagcgccga gcaacagcag taatcctttc atcacgtgac
    11041 ggcgggtgag gcgggtatcg tgcaacgccc ggctggcaac gtcgccaggc acaccgccaa
    11101 gctggttgcg caggttttca acctgctgcc aggcccactg gttatcctga tcctgttcat
    11161 accactgttg ccagcgcgct tcctgttgtg ggctgacgcg ctcgccgctt agcacggcat
    11221 accagtggga agctgaacgc agcgcctgac ggcgggaatc ggttaacaaa ggattcataa
    11281 cccatactcc agacggaaca gcaggcagtg ctcgacggct ttcgccacgt attttttcac
    11341 ggagctgatg gaaacaccga gtttgtgcgc aatctcgctg tatgtcagac catccagttg
    11401 cgaaagcaga aacgcttcac gtgttttgcc gtttagcccg tccagcatgc tgtcgaggag
    11461 ttgtagggtc tcgagttggc tttcgcgttc ctcaggtgaa ggcgctcccc cctccggcat
    11521 aagcgccagc atctccagat acgctttttc cagcgcgttt cggcgaaaca ggtccaccat
    11581 cacgcgtttg gcgatagtgc agaggaagga gcgaggatcg cggatcgtcg agagcgtttc
    11641 gctgaccatt acccgcaaaa aagtgtcctg ggcaatgtca tctgcatcaa aagcagactg
    11701 gagtttgcgc gtcagccagc ttttcaacca gccgtgatgt gtgccataaa gcgactcgaa
    11761 cgttaaggaa gctgtggtag tggcgcggtc agacatgcgg agtgcatcaa aagttaatta
    11821 tcacgtagtc atattaatat gagaatggtt atcattacaa ttggaaataa aattgtttcc
    11881 aatagacatt tttaacatgt tgtttttcta agtgttataa ggtaggtata aaatgggatg
    11941 gagcctctgc ttctggcatg tgtcggtcag aatgactcat gatgtggtct gctattattg
    12001 acatcctcac tgccctaaag gatggggatt tcggtaatgc tgccaactta ctgatttagt
    12061 gtatgatggt gattttaagg tgcttgcgtg gcttccattt ccatcagatg tccttcctgc
    12121 tccgctactg aaggcgtggt gcgtaacggc aaaagcactg ccggacatca gcgctatctc
    12181 tgctctcatt gccgtaaaac atggcaacta cagttcactt acaccgcctc tcagcccggt
    12241 acgcaccaga aaatcattga tatggccatg aatggcgtcg gatgtcgcgc cagtgcacgc
    12301 attatgggcg ttggcctcaa cacggtttta cgtcacttaa aaaactcagg ccgtagtcgg
    12361 taacctcgcg catacaaccg ggcagtgatg tgattgtctg cgctgaaatg gacgaacagt
    12421 ggggctacgt cggtgctaaa tcacgtcagc gctggctgtt ttacgcgtat gacaggatac
    12481 ggaggacggt tgtggcgcac gtcttcggtg aacgcactct ggccacactg gagcgtcttc
    12541 tgagcctgct gtcggccttt gaggtcgtgg tatagatgac ggatggctgg ccgctgtatg
    12601 aatcacgcct gaagggaaag ctgcacgtta tcagcaagcg ttacactcag cgcattgagc
    12661 gacataatct gaatctgaga caacatctgg caaggctggg acggaagtta ctgtcgttct
    12721 caaaatcggt ggagttgcat gacaaggtca tcgggcatta tctgaacata aaacactatc
    12781 agtaagttgg agtcattacc tcctcccgaa ctcaaagcta tcatacagat aattactggt
    12841 taaccctata tagggcattt taaagtgttg tgatcttacc cagcaatagt ggacacgcgg
    12901 ctaagtgagt aaactctcag tcagaggtga ctcacaagac aaaacagttt caaccagtaa
    12961 aaaaacgtaa acaccattcg catgaatttc gtagttaaga tctgaagctt gcagaatgca
    13021 tcggtgttac tgccggagtc cgtgaactta gtctgcatga atcaccacag ctttacatct
    13081 gtcgcagtaa acagcaaaat cagcaaactt acttaattat atcgagtgcg attacaatcg
    13141 ctggcgatgg tacagttggt gtggcggtct cagtccggaa caatttgaaa atcagaatct
    13201 cacttaaaac ggcatccatt ttacaaaaga tggccgtctg tgtatttata atgaatacct
    13261 tctacatgtt tagagtgcat aatccacgac aaggcgatat cctaaaaatt agctagcagg
    13321 caactttgat ggcatagata atgtttttag taaaaaagag ttgactgaat ataatacagc
    13381 ctcttcctac ataagaagga atgtgctcac aaaaaaaacc attagtctgc ttcctgttct
    13441 tacaataatc agtattatgt attttattta tatagcaaaa tatgcttgtt acacctgtca
    13501 taattaggca tggtaaaatt attgactgaa ccctggggga gaaagatgca cagtgattat
    13561 tataatatgg tttttggtga aaaattggcc aatattttat atgaagctaa ctctcaattt
    13621 ttttacgaga gaaatgtcat agaagaggca gtcaacgctt tattctgtga gcgtgaaata
    13681 ataaacaaca agaacataat atagaagctg atgttctttt tgtcagatgt taatcacacc
    13741 aagaaagatg ttgttcagag cgcacttaat attatcattg acattacatc aggtgatatt
    13801 tgagatttta cataaaagat aatctctcaa ggagtcttat ctgaaaacga aatatatagt
    13861 ccctctcgtt ttgtaatatc gattggcttt gttgaatcgg ttacgtaccc cggagccggt
    13921 catattttca ggtaatacac acgctttcag acatttcggc tttcgtcatt ttggtatcat
    13981 gggcatagct tctgctacct gaccatcata atcacgcagc ttcagtgaag cagtgaccac
    14041 ctgtttaacc cggtgtatcg ttatgatctt atccagcaat aatggacacg cggctaagtg
    14101 aaccgccccg ggtttcctgg agagtgtttt atctgtgaac tcaggctgcc agatcatcgt
    14161 ttccgatgga agcataataa gctttttctg cttctgccgg tgggatatgg ccctgccttt
    14221 ccagcaatcg tcgattgtta taccagtcca cccacgtgag tgtggtcagc tcatcatact
    14281 gacacgtgca ttacccctac cgcgataatg caaccgtaca atatagggcg tttcctaaac
    14341 gcagccctca tcactccaat ccaacaagac acagcatttt tgatcacgaa gaaagacaaa
    14401 taatttccgt aaatgtttcc gttgttacaa ataacaacag tgttttacat ttctttggcc
    14461 tcaggcccgc gcatgttgcc attcgatgtc attttcctga ctcgtttctt ctttatggtg
    14521 tagtgtgatc ttcactttat ggatgctaac aatatgaaaa gaaaaattat tcccgtgcta
    14581 atcggttgta cgctctcttt ctctgccctg gcggcacaac ctacggctga gcgctatgtc
    14641 gtcagttttc cagaaggcac gcacgtgaac tacgccggcg catttgccag tgcattcccg
    14701 aacggccttc cggttgggat aggttctggc ctgctgttca ccggtaaaca gggcgatgcc
    14761 ctgacattcg caaccattac cgatcgcggt ccgaacgcgg attcaccgaa agaggggaaa
    14821 aacgaaacta aaattttcgt tacgccagat ttcgcgccgc tgctgatgac gattcgtgta
    14881 caaaacggca aagcggaggc catcgatccc cgtcctctgc atgacgataa aggcgcgatc
    14941 aacggtctgc cgctggcaag cgacgtgatt ggctccacca atgaagtggc cttcagcgac
    15001 acgttacata gactgaaggg ggataatcgc gggctggata cggaagggat cactccggat
    15061 ggtaaaggag gttactggct gtgcgatgag tatggcccgt tcctgattaa catcgacagt
    15121 aaagggaaaa tcctggcaat ccacggtccg caggcggcgg aaggggaaaa agcgattgcg
    15181 ggcggtttgc caaatatcct caaatggcgt caggcgaacc gcggctttga aggcctcacc
    15241 cgtatgccgg acgggcgtat tattgtcgcc gtgcaaagta cgctggatat cgacgctaag
    15301 agcaagaaaa aggcgctgtt tacgcgtctg gtgagcttcg atccggcaag cggaaaaacc
    15361 gcgatgtacg gttatcctat cgacagcgcg gcctacagca aaaacagcga cgctaaaatc
    15421 ggagacatcg tggcgctcga taaccagcac atcctgctga tcgaacaggg aagagataaa
    15481 aacaacagaa tgcgtaacct gatttacgag gtcgatctca ataaggcgag cgacctgtca
    15541 ggcttcgaca agccgggtga gtatccggag tttgatgatg agaaaaccct gtcacagcgc
    15601 ggcattaccc ttgcgcagaa aacgcaggtt gttgatctgc gctccttggg ctggcagcag
    15661 gagaaggctg aagggttggc gctgatcgac agcaaaacgc tggcggtggc gaacgataac
    15721 gactttggcg tgaaggtggc gatgcaacat ccggtcgagg gcaagacatt caaggattat
    15781 cgggtgaacg cggaaggaaa gctgacgctt gacgacaagc aggttgaaac gaccctcaga
    15841 gtgaagccac tggagaaacc ggagtctgac agtgaactgt ggattgtgac gctgccggaa
    15901 gcgttgaaat aagtttataa ggtacggaca gcagtatcgc ttagtttttc aaattcatgt
    15961 atgtgaacaa tattggggtt ccggcgtaga cccccaatga aagcgtcacc acgtgaaaac
    16021 atgcctgcac ggtttacacg tcatctcatg gtggcagccg gttaactttc ctgctacacc
    16081 agtggaaaaa ggccataagc agtccgaaca aaagtaagcg tacagcgagg gccgtattga
    16141 cggggatgtg ttattcagct ggcagtgcta tgcgccacgg aagcagttcg ctgacccggt
    16201 tgaccggcca gtctgctatg acgccaggca catggcgaag gtagctttct ggatccacgt
    16261 cattcagttt gcacgtcccg atcaggctgt acagtagcgc tccccgctca ccaccatggc
    16321 cagagccgaa gaacagggtc ttcatctttt cacgcaacca gctttccagg gatttcaaca
    16381 gcggtttcgt ttttcgctga cgttcagcaa gccgctgctc tgccggcatt ccccttatat
    16441 ccgcctctat ggcgtacaac tgaccgatct gctctagggc ttcttccgtc agtgctgacg
    16501 ggatgcggac gtgcacatcg tggatctttc ggcgggcatg agcccagcag gcagcttccg
    16561 ttatcccacc attgcgatac agctcgttga acccggcgta cgcatccgct tgcagcacac
    16621 cgctgaagca agcaagatga gtctgcggat ggatgccttt tctgtccggg ctgtaagcga
    16681 accacactgc aggtgccaac gctgacccgg cattgcggtc atcacgaaca tacgcccaca
    16741 accgcccggt cttcgtcttc ttattacccg gcagcagtac ctggaccggg gtatcatcgg
    16801 catggagttt gccgtcagtc atgacatagc catgaagcgc ctcttccagc ggagacagca
    16861 gccggcagca tgcatccacc cagcccgaca gcagtgaacg gctcagctcc acaccttgcc
    16921 ggccgtatat ttctgactgg caatacagcg gggtgtgctc tgcatacttc gaggtcagca
    16981 cgcgggccag cagccccggt ccggcgatac cccgctcgat gggccgcgaa ggtgcaggtg
    17041 cctgcacgat ggcatcgcac tgagtacagg catgtttttc ccgtaccgtc cggataaccc
    17101 ggaaggcgct acgcatcgac tccagctgtt cggcggtatc ctcgcccaga tagctcagtg
    17161 aaccgccgca gttcgggcag cacggcgccg caggcaacag tcgcttttcg tcacggggta
    17221 gtgattcagg gaacggctta cgggtgcggg tctgacgcaa cggacgctgt actgccgggt
    17281 catacaccct accagtcagc gtatcgctct ctttctgaag ccggttcaga tcggcttcca
    17341 tttgtgcgat acggcgggag actttttcgg aacgactgcc gaagttcatc cggcggagtt
    17401 tatccagctg cgcctgcaga tggtctattt cgcgctcccg gttgctcagc ttttcctgca
    17461 gggcgtggat cagcgcttcc tgttcggcca ggcgctgttt tagcaggaag atgtcgtcag
    17521 aagagatgtc gttcataagc ccgtatttta ccgggcttat tctgtgacaa ccaggataaa
    17581 gagatttaca gcatggtcag ggaggtcagc agccgcttgg gctgtcgcca gtcgatacct
    17641 tccagcagca tcg
//
LOCUS       DAJOWB010000097        17587 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJOWB010000097 DAJOWB010000000
VERSION     DAJOWB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.097"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(158..2791)
                     /gene="mngB"
                     /locus_tag="OHO85_003503"
     CDS             complement(158..2791)
                     /gene="mngB"
                     /locus_tag="OHO85_003503"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCQ2672827.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEQIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLSERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(2809..4725)
                     /gene="mngA"
                     /locus_tag="OHO85_003504"
     CDS             complement(2809..4725)
                     /gene="mngA"
                     /locus_tag="OHO85_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCQ2672828.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            4894..5616
                     /gene="mngR"
                     /locus_tag="OHO85_003505"
     CDS             4894..5616
                     /gene="mngR"
                     /locus_tag="OHO85_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCQ2672829.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(5720..6589)
                     /gene="sucD"
                     /locus_tag="OHO85_003506"
     CDS             complement(5720..6589)
                     /gene="sucD"
                     /locus_tag="OHO85_003506"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCQ2672830.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(6589..7755)
                     /gene="sucC"
                     /locus_tag="OHO85_003507"
     CDS             complement(6589..7755)
                     /gene="sucC"
                     /locus_tag="OHO85_003507"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCQ2672831.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(7849..9066)
                     /gene="odhB"
                     /locus_tag="OHO85_003508"
     CDS             complement(7849..9066)
                     /gene="odhB"
                     /locus_tag="OHO85_003508"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCQ2672832.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(9081..11882)
                     /gene="sucA"
                     /locus_tag="OHO85_003509"
     CDS             complement(9081..11882)
                     /gene="sucA"
                     /locus_tag="OHO85_003509"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCQ2672833.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPNQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(12183..12899)
                     /gene="sdhB"
                     /locus_tag="OHO85_003510"
     CDS             complement(12183..12899)
                     /gene="sdhB"
                     /locus_tag="OHO85_003510"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCQ2672834.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(12915..14681)
                     /gene="sdhA"
                     /locus_tag="OHO85_003511"
     CDS             complement(12915..14681)
                     /gene="sdhA"
                     /locus_tag="OHO85_003511"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCQ2672835.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(14681..15028)
                     /gene="sdhD"
                     /locus_tag="OHO85_003512"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(14681..15028)
                     /gene="sdhD"
                     /locus_tag="OHO85_003512"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCQ2672836.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(15022..15426)
                     /gene="sdhC"
                     /locus_tag="OHO85_003513"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(15022..15426)
                     /gene="sdhC"
                     /locus_tag="OHO85_003513"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCQ2672837.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            15807..15914
                     /locus_tag="OHO85_003514"
     CDS             15807..15914
                     /locus_tag="OHO85_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672838.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16120..17403
                     /gene="gltA"
                     /locus_tag="OHO85_003515"
     CDS             16120..17403
                     /gene="gltA"
                     /locus_tag="OHO85_003515"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCQ2672839.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT         4177 a         4778 c         4364 g         4268 t
ORIGIN      
        1 atgcgctacg cttgccagtg tctacatcat ctctgcaata tattgaattt gcctgctttt
       61 gtagaccgaa taaagcgttc atgccgtatc cggcatgaac aaagcgccct ttgtcaacaa
      121 tctggccgcg catatgtggc ttcggtttga ttgctattca ggcaagccgg taactgaacg
      181 tccgtgactg gccgggtaaa aaaggccccg atagatttga accttgattt tcctcggtgg
      241 taatgtgttc atccatcatc gtttctgagc aagaaatcac ctcgcgactg aaagcaacag
      301 tcgcatcaca ggttgctgat tcagccggat taaacagccg taaaatcact tcttgtcggt
      361 cttcagcttt cttaagtgcg cttatcaggc atcccactgg gggcattttc aacaaactat
      421 aactttccgg cacgttgaat ccggctttgt tgagcttcat cgcatcccat gggattttgt
      481 tgtaacactg tactggagtc agccatgctc gcgcctgctg agctacacca gcggcggttg
      541 gcgtaccggt ataactcaat aaacttaggc gacaagaaag cagaccacgt agttgtgagt
      601 ccgggactgg cattttaatt cccgaaggcc gcccaggcct taaaagcaga tcttctttgc
      661 ccagtaagcc cacgccacgc agcaacgtaa tggcaaaggt tttcttctct tcaccgatga
      721 cttcaaattc acgtagccct tcgctaaaga cagccatgcc gttacgccct tcctgtaagg
      781 caacatagtt gagcatattc cataccggaa ccggcgcttc tttccagcct tcttgctgcc
      841 agttgttcat tgcactgtcg ttcacggggc gcgttagcga accaaactgc gtatctgcca
      901 gaacactgtc ggtgttaaaa ggtgtaggga tcaggacgcg aaggcgatga tcgtcagcct
      961 ggttatcaag gttgatatcc acatcaatac gtctgctgtt atgactcaga gtgacaacca
     1021 tctccacccc tactctgccc gtggattgcc ggacgctgcg ttctgacaaa ttgagcggca
     1081 ccgccatgtc atagcggata acagccctgc tctgccaggc ctcatgagta atctcgcatt
     1141 gcggtttcgc tgttgcagaa gtcattaccc actcttcttt tgcgggtgaa tagtcatatt
     1201 catcaccatc atcagagctt tcttcaattt gcaacacccg gtcatagcgc acaccgctat
     1261 ctttatctac cagttgtagc gttccatcct cattgagcgc aatttgccag aaagcatttt
     1321 ccagtatccc ttcagcgtca ctttttgccg caatcacgtt gccaggctga ttcgcttcga
     1381 tataaagcgt gcgatagccc atagaaggga caatctggtt gatctgtata tcaaactcca
     1441 taaagggatc gtaattaccg taatgaacta tttgccgatc gattaggcct ggatcgatct
     1501 cacgggcatg gcgaataaaa tacggtacag gctgaccgcg atcgtctcgt agattaaact
     1561 ggctggcgcg cagccgcaca gtggtgttaa taacttcttc ccgcggccag ggcatcaggt
     1621 taaacagcac gagtttgtcg gcatcactct gtggcatgtt atcggcaatt ttgcgcatgt
     1681 agaaacgcag cagattatcc gccatgtctt cagccagttc gaagcgggcg acaatttcgc
     1741 gatgaacttt gtcactgcaa caacaaccga tactgtcgtg ggcatgattt tttaagatct
     1801 ctttccacat tttctccagc aagccgtggt ggtattcaaa acccaacgtc caggccagcg
     1861 ttgccagcgg ttccagcaga ttaacaatct tattttcaat acgtgcgtgg gcaactttga
     1921 tatccatgcg cgtggaaccg atggtgcgat gcacgcgcat atatttgcca tcaataaatt
     1981 cacctttcag ggtcgccaga ctttcacgct gcgcttcaat ctgctcgaat acctcttcaa
     2041 agcggctcat cacaaacttt cgttgaggat aaatttcgcg aagtttctcc atcacttcga
     2101 agatattttg ctgcaatggc atctggtcat gaccattcgg caataaaatc tctttggtta
     2161 cagacgcttt ttccagcacg tcaaaataac tgtcgaggcg tttgcgtaat ccgttttcgt
     2221 cggcaggtaa gtacttaccg atggcataac ccaacggcag cacctgcgcc gtcacttcgc
     2281 taccgtcact gctttgccat aaaaactcgg ttttatcagt accgtggcgc tccgaacatc
     2341 cgcgccagaa catagtgcgg gtaatgccaa atccattgta gatatgcggc agttgcccgg
     2401 acatgccaaa ggaatccggt aaataaccta ttttcatcgg ctcgccaaac gcgaggcagt
     2461 cacgcattcc gtacatcaga ttacggacga tggattccgc agaaacaatc gtggtatcgg
     2521 tctgggtata ccaggggccg ataatcaact taccggcttc tacctgtttc ttcacgcggt
     2581 ctttgttttc cggtttcacc gcgaaataat cttcgaggat cgccgtttgc ccgtcgagta
     2641 cgtaatattt gtattcgttg tcctgttcca gtcggcacag gatctcttcc atattattga
     2701 ccagcagaat gcgtgactct tcggtggtga aataccactc ccgatcccaa tgcatatgcg
     2761 gggtgatgtg aacgcgagat accgctttca tcgtcgtttc ctttttgttt atggcattac
     2821 gccatcagtc agatagttgc catgcttaac cgcgtgacgc cgccagatca ggagaattgc
     2881 agtcgagatt gcagccccca ccaatgccgc accaaaccag ccaattgccg ccataacacc
     2941 gcccgcgcca ttatcatgaa gtaaaaagag cgagaaaatg ccggcaccgg gtgtcgaaag
     3001 gccgatattc atcgcaccga caatagcgcc cgttaccata gagcccagca caaacgaacc
     3061 aataacccgc agcggatctt caatcgccat cgggatcgcc ccttcggtaa tacctgccag
     3121 ccctaacagc caggtggatt tcccggtctc aatttcaaac tctttaaaca ggcgcggtgc
     3181 gagcatcgtg gaagcggtta cggtaaatgc cgaaaccatt ttgacggagg cgaaaatggc
     3241 atacgggccg taaacgccgt tcgccattgc gcccaggcag aatgcataag cggctttgtt
     3301 cactggccct ccaaggtcaa aggaacacat aaaaccgaga atggcaccca gcaacagcgc
     3361 gttacttcct gacagaccgt tcagccaggc ggtaagcgag ttattgatcc aggcgacagg
     3421 ttctcccacc acaaacagca tcagactgcc cgctcccaac gtaccgagca ccgggtagag
     3481 ataaaaagtc aggaatccat tgaatttact gctaagacgc aagtgatttt tcacccagcg
     3541 catcaagtaa ccggctatca atccgccaac gaccgcgccg agaaacccgg agccgatcat
     3601 gttggcggca agtccagccg caaagcctgg cgctaacgcc ggtttatctg ccagagaata
     3661 ggcggtatag gccgcgagca ccggtaccat caaaattccg agcagcccgc cgcccagctt
     3721 gcggtacatc cacagccagg agttttcttc attaaacaga tcttgtagcc cgaagatttg
     3781 cgacagtaat accgcgaccg ccagcaccgt gccccccgcg acaatcaacg gtacggcaaa
     3841 agagattccg ctcaacagtg cctgtttcag ctccgttttg acacttttca ccggttgcgt
     3901 atcttgctgt acggtacgcg tctcatcgct acgcttgagg gtaagcgctt gttggatcaa
     3961 cgcttctgca tggcgaatcg gctcggcaac aggcactgaa agcgcgggaa tgccattaaa
     4021 acgctcactc tccttgatgg cgacttcagc cgcaaaaata caggcggttg cactattgag
     4081 ttgatccgcc gttaaacgcc cttcaatgcc gttagcgcct tgtttttcaa cgtaaacatt
     4141 tacgccgagt ttgcgtccgg ctttttccag atattccgca gccatatagg tgtgagcaat
     4201 acccgccgga caggccgtta cgcagacgat agttggcgcg ttggaaaaag aggcagaagg
     4261 ttgcgtgcct cccttgtcat ccagcgccga gagcaactca tcaggcgtcg tcgccgactg
     4321 tatacgcgcc cgaatctcat catccgccag gcgcgtggtc agcgctgtca gcagttgcat
     4381 atgcgtagta cccgcttcat tggggggaat cgccagcagc accactaaat caaccgcttc
     4441 cgggccatca acgccttccc actgaagcgg ctcgctgagt gtggcgaccg caaacgccgc
     4501 ttcttttacc gcagcagttt tgccatgcgg cacagccaac ccttcaccta aggcggtcgg
     4561 gccaaggctt tcacggcgat acacttcttc cagaaattgc tcagtactgg aaattttccc
     4621 cagagcagca agacgttgag tcaacgcgtg gatggcctct tcacggctgg taaagcgcgc
     4681 attcagacac aacgcatcgc ggtgggtcag agtcgtcaga ttcatcatga tcctcacttg
     4741 atcgttttta tagtcgcgcc agtgtgcccg ataaaacaat accattatgt gatcaatttc
     4801 acttgattga aattatttgt attgtatttg tattatttac aggatgaatt tatcgggcat
     4861 aatggctaaa ttcgtattaa tgagatacta aaaatgggac acaagccctt ataccggcag
     4921 attgccgatc gcattcgtga acaaattgcg cgtggcgagc ttaaacctgg cgatgcgtta
     4981 cctaccgaat cggcattaca aacagagttt ggcgtcagcc gggttacggt gcgtcaggct
     5041 ttgcgccagt tagtcgagca acagatcctc gaaagcattc agggcagcgg gacttacgtc
     5101 aaagaagagc gggtcaatta cgatattttt cagttaacca gttttgatga aaaactgtcg
     5161 gaccgtcacg tcgatacaca cagtgaagtc ctgatatttg aagtgattcc ggctgacgat
     5221 tttcttcagc aacagctaca aatcaccgcg caggatcgtg tgtggcatgt gaagcgcgtg
     5281 cgctatcgca agcaaaagcc aatggcgctg gaagaaacct ggatgccgct tgcgttgttc
     5341 ccggatctca cctggcaggt catggaaaat tcaaaatatc actttattga agaagtgaag
     5401 aaaatggtta tcgatcgtag cgaacaggaa atcattcctc tgatgccaac cgaagagatg
     5461 agccgtctgc ttaatatcag ccagacaaag ccgattcttg aaaaagtatc gcgtggatat
     5521 ttagttgatg gtcgggtgtt tgaatatagc cgcaacgctt ttaataccga tgactataaa
     5581 tttaccctga tagcgcaacg aaaatcatcg cgataagcac aaaaaaggcc atcatacgat
     5641 ggccaaccat gtcgaaaccg acagcccttc gccattcctg tagagggaag cggcgagggc
     5701 tatttcttat tacagatatt tatttcagaa cagttttcag tgcttcaccg atatccgcca
     5761 ggctgcgaac ggttttcacg cctgcggctt ccagagcagc gaatttctca tccgcagtcc
     5821 ctttcccacc ggcaatgatg gcacccgcgt ggcccatacg tttgcctttc ggcgcagtca
     5881 caccagcgat gtaacccaca actggcttgg taacatgctc tttgatgtac gctgccgctt
     5941 cttcttcagc gctaccgccg atctcaccga tcattacgat cgcttcggtc tgcggatctt
     6001 tttcgaacat ttcgagaatg tcgataaagt tagagccagg gatcgggtca ccgccgatac
     6061 caacacaggt cgactgaccg aaaccgtaat ccgtggtctg tttaaccgct tcataggtca
     6121 gtgtaccgga acgggaaacg atacccactt tacccggttt gtgaatgtga cccggctgga
     6181 taccgatttt gcattcaccc ggagtgataa cgcccgggca gttcgggccg atcatacgaa
     6241 cgccggcttc gtccagtttc actttcacag tcagcatatc cagcgtcggg atgccttcag
     6301 tgatggtgat aatcagtttg atgcctgcat caatagcttc cagaatggag tctttgcaga
     6361 acggtgctgg tacgtagata acagaagcgg tagcgccagt agcagcaaca gcttcacgca
     6421 cggtgttgaa caccggcagg ccgaggtggg tagtaccgcc tttacctggg gttacgccgc
     6481 caaccatttt agtgccgtat gcaatggcct gttctgagtg gaaagtcccc tggctaccag
     6541 taaagccctg gcagataacc ttggtgtttt tatcaattaa aatggacatt atttcccctc
     6601 cactgcggca acaacctgct gagctgcatc cgtcagacct tttgctgcaa taatattcag
     6661 gccgctgtca gccagtttct tcgcgccgag ttcggcgttg ttaccttcca gacgtaccac
     6721 gaccggtacg ttaacaccca cttctgctac cgcgccgatg ataccgtcag cgatcaggtc
     6781 gcaacgaacg ataccgccga agatgttaac cagaacggct ttcactttgt cgtcagagag
     6841 gatgattttg aacgcttcgg ttacacgttc tttggttgcg ccgccgccaa cgtcaaggaa
     6901 gttagccggt tcgccgccgt gcagtttaac gatgtccatc gtacccatcg ccagacctgc
     6961 gccgttaacc atacaaccga tgttaccgtc cagcgcaacg tagttcagtt cccactgtgc
     7021 agcctgtgct tcacgcggat cttcctgcga ctggtcacgc atttcgcgca gatcaggctg
     7081 gcggaacagt gcgttgccgt cagcgcccag tttgccgtcg aggcaaatca gatcgccctg
     7141 tttggtgatg accagcgggt tgatttcgat caacgccagg tcgcgctcca ggaaaatggt
     7201 cgccaggccc atgaagattt tggtgaactg ctgaaccagt ttaccttcca gacccagttt
     7261 gaacgccagc tcgcgtccct gatacggcat cgggccagtc agcggatcaa gcgcaacttt
     7321 gtggatcagg tgcggagttt cttccgccac tttttcgatt tccacgccgc cttcggtgga
     7381 ggccataaag accacacgac gggaactacg gtctacaacg gcaccgagat acagctcttt
     7441 agcgatatcg gtcgctgctt caaccagaat ctggttaacc ggttggccat tggcatctgt
     7501 ttgatacgtt accagacgct tgccgagcca gttttctgca aaagcacgga tgtcttcttt
     7561 gctgtttaca actttcacac cgcccgcttt accgcggcca ccagcgtgaa cctgacattt
     7621 cactacccac gggccggcac cgatttttga agcggcttct tctgcttcgc gcggagtagt
     7681 acaggcataa cccaccggtg ctggtaagcc atagcgggca aaaagttgtt ttgcctgata
     7741 ttcatgtaag ttcatgtgtt ctgtccatcc ttcagtaatc gttatctttt aaaccgtaga
     7801 ccgacaccct atccacgtcg gtctacagtg caggtgaagc ttaaactact acacgtccag
     7861 cagcagacgc gtcggatctt ccagcaactc tttgatcgtt accaggaagc ccacggattc
     7921 gcgaccatcg atcagacggt gatcgtagga cagcgccagg tacatcatcg gcaggatctc
     7981 aacctgacca ttcaccgcca tcggacgatc tttgatagcg tgcataccca gaattgcgct
     8041 ctgcggcggg ttgatgatcg gcgtagacat cagggaaccg aacacaccac cgttggtgat
     8101 ggtgaagtta ccaccggtca gatcttcaac ggtcagcttg ccgtcacggc ctttgactgc
     8161 cagctctttg attttcttct cgatgtctgc catgccgagg gtatcgacat cacgcagtac
     8221 cggtgtcacc aggccgcgcg gcgtagaaac cgccatgctg acgtcgaaat agttgtggta
     8281 aaccacgtca tcgccgtcga tagacgcgtt cacttccggg taacgtttca gggcttcaac
     8341 caccgctttc acgtagaagg acataaagcc cagacggatg ccgtggcgtt tttcaaacgc
     8401 ttcaccgtac tgcttacgca gatccataat cggcttcatg ttgacttcgt tgaacgtggt
     8461 cagcatggcg gtggagtttt tcgcttccag cagacgctct gccacacgct tacgcaggcg
     8521 agtcatcggg acacgttttt cactacgtgc agccagagcc ggttgcgccg ccggagcagc
     8581 cgctgccgga gcagactctt tcgccggggc tttcgccaga tgtttttcca catcttcacg
     8641 agtcagacga ccacccacac cggtgccttt aatggcgctg gcgtcgagat tgtgttcagc
     8701 cagcaggcga cggatcgccg ggcttaacgc atcgttgttt tgctcttcca gagatgcctg
     8761 ctggcgttgc gccggagtgg acgctttctc ttcagatttg gcgctggttt ctttaccggc
     8821 gctgttgcct tcacgcaggc gaccaaggat ctgacgagac gttaccgttg taccttcatc
     8881 ttccagaacc gcatccagaa tgccgtctgc tgatgccggt acttccagta ccactttgtc
     8941 agtttcgatt tctaccagca cttcatcacg tacgactgcg tcgccgggtt ttttatgcca
     9001 ggttgcgacg gtggcatcgg ctacggattc aggcaggtca gggaccagaa tatctacgct
     9061 actcattgtg tatcctttat ttattcgacg ttcagcgcgt cattaaccag atcttgttgc
     9121 tgtttctggt gaacggacat ataccctacc gccggagagg cggaggccgg gcggcctgca
     9181 taacgcagag aagccccaaa cggaatcact tcacggaaat gatgctggct gcagtaccat
     9241 gcgccctggt tgagcggctc ttcctggcac cagacaaaat ccttgacgtg agcaaactgt
     9301 tgcaacactt cctgcatcgc tttatgcggg aacgggtaga gttgctcgat acgcacaatg
     9361 gcgacatcgt gttgattgtt cttacgacgc tgttccagca ggtcgtaata aaccttacca
     9421 gaacacatca ctacgcgctt cacgcccttc ggatcaagct cgtcgatttc accgatggct
     9481 ggcaggaagg tgccgttcgc cagttcttcg aggctggaaa ccgccagcgg atgacgcagc
     9541 agggatttcg gcgacatcac gaccagcgga cgacgcatcc cgcgcagcgc ctgacgacgc
     9601 agcatgtggt aaacctgtgc cggggtagac ggtacgcaca cctgcatgtt ttgctcagca
     9661 caaagttgca gataacgttc cagacgcgcg gaggagtgct ccggcccctg cccttcgtaa
     9721 ccgtgcggca gcaacatcac cagaccacac atccggcccc atttctgctc gccagaggag
     9781 atgaactggt cgataaccac ctgcgcaccg ttggcgaagt caccgaactg cgcttcccag
     9841 atggtcagag tgcgtggttc tgcggtggca taaccgtatt caaacgccag cactgcttct
     9901 tcagacagta cggagtccca gacgcggaac gcgccctgcc cgttatggat gtgttgcaac
     9961 ggcgtgtaag tggaaccgtt agactggttg tggatcaccg cgtggcggtg gaagaaggta
    10021 ccgcgaccgg agtcttcacc cgacaggcga accggaatgc cttcgtcaac cagcgtggcg
    10081 taagcgaggt tttccgcacc gccccagtcg aacagtttct caccggcagc catcgcctgg
    10141 cgatcgccat aaatcttggc aacgcgagac tgcatttcaa ctgcttccgg caccgtgctg
    10201 atgcgtttcg ccagctcctg caggcgcttc atctcaactt tgttcgggta ctcttcgtcc
    10261 cattcgtggt tgagatacgg cgaccaggtg aaagagtgca tgttcatcgg acgccactct
    10321 gccactacgc aatcgccagc atccagcgca tcgcggtaca ggttaaccat ctcggtggca
    10381 tcttccagcg tcgccacttt ttcctgctcc agcttgtcag catagatttt gcgcggcgtc
    10441 ggatgttttt tgattttctg atacatcagc ggctgggttg cgctcggctc gtcggcttcg
    10501 ttgtggccgt gacggcggta gcacaccagg tcgatgaaga catcacgttt aaaggtgtta
    10561 cggaaatcga gcgccagacg ggtcacaaag gcaacggctt ccggatcatc cgcgttaacg
    10621 tggaaaatcg gtgcctgaac catcttaccg atatcagtac agtacggtgt agaacgagca
    10681 tccagcggat tagaggtggt gaaaccaacc tggttgttga taacgatacg taccgtaccg
    10741 ccaacttcat aaccacgcgc tttcgacatg ttcagagttt cctgaaccac accctgcccg
    10801 gtcactgcgg cgtcaccgtg gatggtgatt ggcagcactt tgttgctgct cggctcatca
    10861 agtctgtcca gacgggcacg aacagaaccg ataactaccg ggcttacaat ctcaaggtga
    10921 gacgggttaa acgccagcgc caggtgcacc aggccgccat cggtctggaa gtcagacgag
    10981 aagcccatgt ggtatttcac gtcacccgtg ccgaggtgtt ctttatgttt accggcgaac
    11041 tcgtcgaaca agtcttgcgg ttttttaccc agcacgttca ccagcacgtt cagacgacca
    11101 cggtgcgcca tcccgagaac cacttcgcgg gtgccgctgt tgccagcgtg tcggatcatc
    11161 tctttaagca tcgggattaa cgcgtcaccg ccttccagcg agaagcgttt tgcgccaggg
    11221 aattttgcgc cgaggtaacg ttcaaggcct tcagcggcgg tcagttcgct taagaagcgt
    11281 tttttctctt cgctattgaa agtcgcgcga ccagactcga tacgctgttg gatccagcgt
    11341 ttttcttcgg tgctggtaat gtgcatatac tcggcaccaa tcgggccaca gtaggtttgc
    11401 ttgagggctt ccagcagctc gccgagtttc atggtttctt tgccgctggc aaatgaaccg
    11461 acgttgaagg tctcctggaa gtctgcttcg gtcagatcgt ggaaagacgg atccagatcg
    11521 gccactttat cttgctgcca cagtcccagc ggatcgagat tcgcatgctg gtgaccacgg
    11581 aagcggtatg cgttaatgag ctgcaggact ttaacctgct tcacattggt gtcagggtcg
    11641 gagatcgttg aagagtaacg tgaagcgtct ttcgccaggc ggcggaaata ttcacgcgtt
    11701 tgagagtgga attgattcgg tttgactccc gtaccaggta actgctggaa cgtcgaacgc
    11761 cagttagcgt caaccgagtc aggatcggtt aagaagtctt catagagctg ttctatccag
    11821 ctctggtttg cgccagagag gtaagaagag tccaaccagg ctttcaaagc gctgttctgc
    11881 atcgtgatcc cttaagcatc ttttttatgc ttacttcgcc gtggatacta ccacgcacag
    11941 tgatgtgcgt gccggggttc acttgcgagc tcttgtctat gtagtggctc gcgaaggaac
    12001 ctttaaaaac tgtcttagtg tcaggggcag tttttaaaga tttcctgcat ttctttgtta
    12061 cgcctgatgc gctgcgctta tcaggcctac gggtaatgac ttgtaggccg gataaggcgt
    12121 tcacgccgca tccggcactg gttgcctgat gcgacgcttg cgcgtcttat caggcctacg
    12181 gtttacgcat tacgttgcaa caacatcgac ttaatatggc cgatggcgcg cgtcgggttc
    12241 agccccttcg gacatacact gacgcagttc atgatgctgt gacagcggaa tacgctgaat
    12301 gcatcgctca aaccgtcgag gcggctgtca gtctcggtat cacggctatc gatcaggaaa
    12361 cgatatgccg ctaacaagcc tgccgggccg ataaacttat cgggattcca ccagaaagac
    12421 ggacaagagg ttgaacaaca tgcgcagaga atacattcat acagcccgtc gagtttttcg
    12481 cgctgctctg gcatctgtaa atgctcgcga gctggcggat tttgtccatt attcaacagg
    12541 taaggcttaa ttttctcata ttgcgcatag aattgtccca tgtctaccac caaatcgcgg
    12601 atcaccggta aacctggcag cgggcgaatc acaatcttct tgcccggctg gttgagtgcc
    12661 gaaatcgggg taatacaggc cagaccattc ttgccgttca tgttcagacc gtcggaaccg
    12721 cacacacctt cacggcagga gcggcggaac gacaggctgg gatctttctc tttcagctgg
    12781 ataagcgcat ccagcagcat catgtcgcga ccttcttccg cttccagggt gtaatcctgc
    12841 atacgcggag catcatcaac atccgggtta tagcgataaa ttgaaaactc gagtctcatt
    12901 ttcctgtctc cgcattagta agtacgaatc ttcggcggga atgccgggcg cagtttcggt
    12961 tccatgttga cgcttcggcg cgtcatggat tccgactctg gcagatacag ggagtggcac
    13021 agccagtttt catcatcacg atccgggaag tcgaagcggc tatgtgcgcc acggctttcg
    13081 gtacggaagt tggcagaaac agccgttgca tacgccgttt ccatcaggtt atccagttcc
    13141 aggcactcaa cgcgctgggt gttgaactcg ctggaagtgt catccagacg ggcatttttc
    13201 agacgctcgc ggatcacttt caactgctca agccctttcg ccatcgcatc accttcacgg
    13261 aagaccgaga agttatgctg catacattct tgcagcgctt tacgaatcgc caccggatct
    13321 tcaccgttac gattattgtt ccagcggttc agacgatcca gagatgcttc aacatcagac
    13381 tcgctggcat cgcgcaatgc gccctgttcg gcgatagact cttgcagatg cagacccgct
    13441 gcacgaccaa agaccaccag gtccagcagc gagttgccgc ccagacggtt agcgccgtgt
    13501 accgatacac aagcgatttc accaacggca aacagtcccg gaacaaccac atcttcgcct
    13561 ttctcgttca ccgtcagcgc ctgaccggtc actttggtcg gaataccgcc catcatgtag
    13621 tgacaggttg ggataaccgg aatcggctct ttcaccggat caacgtgagc gaaggtacgg
    13681 gagagttcca ggatacccgg caggcgggat tcgagaactt ctttacccag gtggtcgagt
    13741 ttcagtttcg cgtgtggccc ccacggacca tcacagccgc gaccttcacg gatttcgatc
    13801 atgatggaac gcgccaccac atcacggccc gccaggtctt tggcgttcgg cgcataacgc
    13861 tccataaagc gttcgccatg tttgttcagc agataaccgc cttcaccacg gcaaccttcg
    13921 gtgaccagta cgcccgcacc ggcaataccg gtcgggtgga actgccacat ttccatatcc
    13981 tgcaccggta cgcctgcgcg gatagccatg ccgacgccgt cgccagtgtt aatgtgggcg
    14041 ttagtggtgg actgataaat acgccctgcc ccgccagtcg ccagcacggt agcgcgggct
    14101 ttgaaataaa ccacttcacc agtttcgatg cacagtgcag tacaacccac caccgcgcca
    14161 tcctggtttt tcaccagatc cagcgcatac cactcggaga aaatagtggt gtggtttttc
    14221 aggttctgct gataaagggt atgcaacagt gcgtgaccgg tacggtcagc tgctgccgca
    14281 gtgcgtgccg cctgctcgcc gccgaagttt ttcgactgac cgccaaacgg acgttgatag
    14341 atacgaccat catcaagacg cgagaacggc aggcccatgt gttcgagttc cagaatcgct
    14401 tccggcccgg ttttacacat atattcaatc gcgtcctggt caccgatata gtccgaacct
    14461 tttacggtgt cgtacatatg ccattcccag ttatcttcat gggtattacc cagcgcaacg
    14521 gtaatgccgc cttgcgcaga aacggtatgg gaacgggtcg ggaagacttt agagagcagc
    14581 gcacaggttt gaccgctctg ggaaatttgc agcgccgcgc gcatacctgc gccaccggca
    14641 ccaatcacaa ctgcatcaaa ttctctgact ggcaatttca tcacacaccc cacaccacaa
    14701 cgaatccata aatcacgtaa accaccagag caacgacaat caccagttgc agcatcaggc
    14761 gcaaggccag cggtttaacg tagtcggtca acacctgcca catgccgatc caggcatgga
    14821 tcaagataga aaacagcgcc agcagggtga acactttggt gaacgcagag gcgaagaaac
    14881 cgatccagac ttcatatgtc agctcgccac tggtagcgaa aaaaccgacc atataaatga
    14941 tgtagagcgt caggacgata gcggtagcgc gaacgaggat gaaatcatgt acgccattgc
    15001 gtcctaatgc ggaggcgttg cttaccatac gaggactcct gcgagaagtg aaagcacgac
    15061 agtaataaca aaggagattt tggcggagcg tttacccgct tcgaatgttt cttccagata
    15121 gccaaaatcc atcatcatgt ggcgaatacc tacgacgacg tgatacgcca gagcggtaag
    15181 gatgccccac atgataaatt tgacgaagaa gctgcccata atcgcggaag cttgctcgaa
    15241 accttcaggg gaagagaggc tggtacccag aagccacagc aggatgccca ctgcaacaaa
    15301 ggtgatcaca ccggaaacgc gatggagaat ggacgctatc gccgtgatgg ggaaccggat
    15361 ggtctgtagg tccagattaa caggtctttg ttttttcaca tttcttatca tgaataacgc
    15421 ccacatgctg ttcttattat tccctgggga ctacgggcac agaggttaac tttctgttac
    15481 ctggagacgt cgggatttcc ttcctccggt ctgcttgcgg gtcagacagc gtcctttcta
    15541 taactgcgcg tcatgcaaaa cactgcttcc agatgcgaaa acgacacgtt acaacgctgg
    15601 gtggctcggg attgcagggt gttccggaga cctggcggca gtataggctg ttcacaaaat
    15661 cattacaatt aacctacata tagtttgtcg ggttttatcc tgaacagtga tccaggtcac
    15721 gataacaaca tttatttaat ttttaatcat ctaatttgac aatcattcaa caaagttgtt
    15781 acaaacatta ccaggaaaag catataatgc gtaaaagtta tgaagtcggt atttcaccta
    15841 agattaactt atgtaacagt gtggaagtat tgaccaattc attcgggaca gttattagtg
    15901 gtagacaagt ttaataattc ggattgctaa gtacttgatt cgccatttat tcgtcatcaa
    15961 tggatccttt acctgcaagc gcccagagct ctgtacccag gttttcccct ctttcacaga
    16021 gcggcgagcc aaataacaaa cgggtaaagc caggttgatg tgcgaaggca aatttaagtt
    16081 ccggcagtct tacgtaataa ggcgctaagg agaccttaaa tggctgatac aaaagcaaaa
    16141 ctcaccctca acggggacac agctgttgaa ctggatgtgc tgaaaggcac gctgggtcaa
    16201 gatgttattg atatccgtac tctcggttca aaaggtgtgt tcacctttga cccaggcttc
    16261 acttcaaccg catcctgcga atctaaaatt acttttattg atggtgatga aggtattttg
    16321 ctgcaccgcg gtttcccgat cgatcagctg gcgaccgatt ctaactacct ggaagtttgt
    16381 tacatcctgc tgaatggtga aaaaccgact caggaacagt atgacgaatt taaaactacg
    16441 gtgacccgtc ataccatgat ccacgagcag attacccgtc tgttccacgc tttccgtcgc
    16501 gactcacatc caatggcagt catgtgtggt attaccggcg cgctggcggc gttctatcac
    16561 gactcgctgg atgtaaacaa tcctcgtcat cgtgaaattg ccgcgttccg cctgctgtcg
    16621 aaaatgccga ccatggccgc gatgtgttac aagtattcca ttggtcagcc atttgtttat
    16681 ccgcgcaacg atctctccta tgccggtaac ttcctgaata tgatgttctc cacgccgtgc
    16741 gaaccgtatg aagttaatcc gattctggaa cgtgctatgg accgtattct gatcctgcac
    16801 gctgaccatg aacagaacgc ctctacctcc accgtgcgta ccgctggctc ttcgggtgcg
    16861 aacccgtttg cctgtatcgc agcaggtatt gcttcactgt ggggacctgc gcacggtggt
    16921 gctaacgaag cggcgctgaa aatgctggaa gaaattagct ccgttaaaca cattccggaa
    16981 tttgttcgtc gtgcgaaaga taaaaatgat tctttccgcc tgatgggctt cggtcaccgc
    17041 gtgtacaaaa attacgaccc gcgcgccacc gtaatgcgtg aaacctgcca tgaagttctg
    17101 aaagagctgg gcaccaaaga tgacctgctg gaagtggcta tggagctgga aaacatcgcg
    17161 ctgaacgacc cgtactttat cgagaagaaa ctgtacccga acgtcgattt ctactctggt
    17221 atcatcctga aagcgatggg tattccgtct tccatgttca ccgtcatttt cgcaatggca
    17281 cgtaccgttg gctggatcgc ccactggagc gaaatgcaca gtgacggtat gaagattgcc
    17341 cgtccgcgtc agctgtatac aggatatgaa aaacgcgact ttaaaagcga tatcaagcgt
    17401 taatggttga ttgctaagtt gtaaatattt taacccgccg ttcaaatggc gggttaattt
    17461 ttatatgcct aaacacaaaa aattgtaaaa ataaaatcca ctatcggaca ttcatatatt
    17521 ctaaaaaaaa atatatcggt ataaattata aacaaatcga tgatttttaa tcatctatct
    17581 tcctctc
//
LOCUS       DAJOWB010000098        17408 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJOWB010000098 DAJOWB010000000
VERSION     DAJOWB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.098"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            71..325
                     /gene="bssS"
                     /locus_tag="OHO85_003516"
     CDS             71..325
                     /gene="bssS"
                     /locus_tag="OHO85_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCQ2672840.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            440..1558
                     /gene="solA"
                     /locus_tag="OHO85_003517"
     CDS             440..1558
                     /gene="solA"
                     /locus_tag="OHO85_003517"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCQ2672841.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIHHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1606..1719
                     /locus_tag="OHO85_003518"
     CDS             1606..1719
                     /locus_tag="OHO85_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCQ2672842.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            1980..2546
                     /locus_tag="OHO85_003519"
     CDS             1980..2546
                     /locus_tag="OHO85_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCQ2672843.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2550..3125
                     /locus_tag="OHO85_003520"
     CDS             2550..3125
                     /locus_tag="OHO85_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCQ2672844.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3167..4219)
                     /locus_tag="OHO85_003521"
     CDS             complement(3167..4219)
                     /locus_tag="OHO85_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCQ2672845.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4444..5364
                     /gene="lpxL"
                     /locus_tag="OHO85_003522"
     CDS             4444..5364
                     /gene="lpxL"
                     /locus_tag="OHO85_003522"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCQ2672846.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5536..6762
                     /gene="mdtG"
                     /locus_tag="OHO85_003523"
     CDS             5536..6762
                     /gene="mdtG"
                     /locus_tag="OHO85_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HCQ2672847.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            6845..7219
                     /locus_tag="OHO85_003524"
     CDS             6845..7219
                     /locus_tag="OHO85_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCQ2672848.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(7220..7447)
                     /locus_tag="OHO85_003525"
     CDS             complement(7220..7447)
                     /locus_tag="OHO85_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCQ2672849.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(7620..10163)
                     /gene="mdoH"
                     /locus_tag="OHO85_003526"
     CDS             complement(7620..10163)
                     /gene="mdoH"
                     /locus_tag="OHO85_003526"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCQ2672850.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(10156..11691)
                     /gene="mdoG"
                     /locus_tag="OHO85_003527"
     CDS             complement(10156..11691)
                     /gene="mdoG"
                     /locus_tag="OHO85_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCQ2672851.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            12085..13242
                     /gene="mdoC"
                     /locus_tag="OHO85_003528"
     CDS             12085..13242
                     /gene="mdoC"
                     /locus_tag="OHO85_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCQ2672852.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(13250..14731)
                     /gene="clsC"
                     /locus_tag="OHO85_003529"
     CDS             complement(13250..14731)
                     /gene="clsC"
                     /locus_tag="OHO85_003529"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCQ2672853.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(14673..15206)
                     /gene="ymdB"
                     /locus_tag="OHO85_003530"
     CDS             complement(14673..15206)
                     /gene="ymdB"
                     /locus_tag="OHO85_003530"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCQ2672854.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(15301..15612)
                     /locus_tag="OHO85_003531"
     CDS             complement(15301..15612)
                     /locus_tag="OHO85_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2672855.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(15733..16065)
                     /gene="csgC"
                     /locus_tag="OHO85_003532"
     CDS             complement(15733..16065)
                     /gene="csgC"
                     /locus_tag="OHO85_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCQ2672856.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(16124..16579)
                     /gene="csgA"
                     /locus_tag="OHO85_003533"
     CDS             complement(16124..16579)
                     /gene="csgA"
                     /locus_tag="OHO85_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCQ2672857.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(16620..17075)
                     /gene="csgB"
                     /locus_tag="OHO85_003534"
     CDS             complement(16620..17075)
                     /gene="csgB"
                     /locus_tag="OHO85_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCQ2672858.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         4353 a         4437 c         4127 g         4491 t
ORIGIN      
        1 aagttgcttc agataaaaac gcattgaacc tcgaataacg ttgtctagta acacgaatta
       61 gggggccatg atggaaaaaa ataatgaagt cattcagact catccgctcg tagggtggga
      121 catcagcacc gttgatagct atgatgcgct gatgttgcgt ttgcactacc agaccccaaa
      181 taagtccgag caggaaggga ctgaagttgg tcagacgctc tggttaacca ctgatgttgc
      241 cagacaattt atttcgatat tagaagcagg aatcgccaaa attgaatccg gtgatttccc
      301 ggtaaacgag tatcggcgtc attaacatga catgaccatc cacccaaaag gcacctcacc
      361 ggtgccttta ccatttctgg gttgtatatc tgcattcagt taattactct gcttaaacca
      421 tgagagcaga gagaaaaaaa tgaaatacga tctcatcatt attggcagcg gttccgtagg
      481 cgctgccgcc gggtattatg caacccgcgc cggtttaaac gtgctaatga ccgacgccca
      541 tatgccaccg catcaacacg gcagccacca cggcgatacg cgattaattc gccatgctta
      601 tggtgaaggc gaaaagtatg tcccgttggt cctccgcgcg caaacgctgt gggatgaact
      661 ttcccgccac aacgaagatg atcccatttt tgtacgctct ggtgtcatta acctcggccc
      721 ggctgactcc gcatttctcg ccaacgtcgc ccacagcgcc gaacagtggc aactcaacgt
      781 tgaaaagctc gatgcgcaag ggattatggc ccgctggcca gaaatacgcg tcccggacaa
      841 ctacatcggc ttatttgaga ctgattccgg ttttttgcgc agcgaactgg cgattaaaac
      901 ctggatccaa ctggcgaagg aagcgggctg tgcgcaactg ttcaactgcc cggtcaccgc
      961 aattcatcat gacgatgatg gcgtaactat tgaaacggtt gacggtgagt atcaggcgaa
     1021 aaaagcgatt gtctgcgcgg gaacatgggt aaaagacctg ctcccggagc tgcctgtcca
     1081 gcccgtacgt aaagtatttg cctggtatca ggccgatggc cgctatagcg tgaagaataa
     1141 attcccggcg tttaccggtg aactgcccaa tggcgatcaa tattatggtt ttccggcaga
     1201 aaacgacgcg ttgaagattg gcaaacataa cggaggccag gttatccatt cagcggatga
     1261 acgtgttccg tttgcggaag tggtcagcga tggttcggaa gccttcccgt tcttgcgcaa
     1321 tgtattgccg ggtatcggtt gctgcctgta cggcgctgcc tgcacctatg ataattcgcc
     1381 tgacgaagat tttattatcg ataccctacc cggccacgat aatacactgc tcattaccgg
     1441 cctgagtggg cacggtttta aatttgcgtc agttttaggg gaaatagctg ccgattttgc
     1501 gcaagacaaa aaaagcgatt ttgatttgac gccattcagg ctttcccgct tccaataatc
     1561 atcattacgg cctccagaat gaggccgttt ttacaggagt atttaatgcg tcggctgttg
     1621 cactatctca tcaataatat tcgcgagcat ctgatgcttt atctttttct ctggggattg
     1681 ttggcaatta tggatctgat ctatgtgttt tatttttagt tttccgcact gacataaact
     1741 gacaaatatt taatctccct ttcatttagc gtgaaaaatt gaatgagatt attcctgctt
     1801 ctacagcact caccagacat ctctgatgct gaacccacta aacttatgaa gattctttca
     1861 ttattaattc aaaattattg aaataaaaat aaatatcatt caatttgtta tttttgtgtt
     1921 gcaattcatt ctccgacacg ccattttctt tctctcgcaa attcgtattg gatgcactta
     1981 tgtcattcac aaatacccct gaacgctatg gtgttatatc tgcggccttc cattggctaa
     2041 gcgcaattat tgtctacggc atgtttgctt taggtctgtg gatggtcacg ctcagttatt
     2101 acgatggctg gtatcacaaa gcacccgaac tgcataaaag catcggtatt ttgttaatga
     2161 tggggctggt tattcgcgtt ctgtggcgtg tcatatctcc accgccgggt ccgctgccga
     2221 gttattcgcc catgactcgt cttgccgcaa aggctggaca tctcgcattg tacctattgc
     2281 tgttcgccat cggcatcagc ggctatctga tctcaactgc cgatggtaaa ccgatcagcg
     2341 tttttggctg gtttgacgtc cccgcgaccc ttgccgacgc tggcgcacag gcagactttg
     2401 ccggtgccct gcatttttgg ctcgcgtgga gcgtcgtagt actgtccgtt atgcacggat
     2461 ttatggccct gaagcatcat ttcatcgata aagacgacac tctgaagcgc atgctgggaa
     2521 agtcgtcatc tgactatgga gtataaaaaa tgaaaaaaag cctgcttggt ttaaccttcg
     2581 cgtccctgat gttctctgcc ggttcagcgg ttgccgccga ttacaaaatt gacaaagaag
     2641 gtcagcacgc ctttgttaat ttccgcatcc agcaccttgg ctatagctgg ttatacggca
     2701 cctttaaaga tttcgacggt acttttacct ttgacgaaaa aaatccggct gccgataaag
     2761 tgaatgtgac aattaacacc accagcgtcg atactaatca cgccgaacgc gataaacatc
     2821 ttcgcagtgc agatttcctc aataccgcaa aatatccaca ggcaacattc acctccacca
     2881 gcgtgaagaa agacggtgac gaactggata ttaccggcga tctgacgctg aatggcgtaa
     2941 ccaaacccgt cacgctggaa gcgaaattaa ttggtcaggg cgacgaccca tggggtggta
     3001 aacgtgcagg cttcgaggcc gaaggcaaaa ttaagctcaa ggacttcaat atcaagacag
     3061 atttaggtcc agcttctcag gaagtggatc tgattatttc agtggaaggc gtacagcaga
     3121 agtaagagtg aaatgttgcc ggatggcacg catccggcaa tgatatttat tccgttggat
     3181 caggaatgcc cagtgttgta ttcagacgtc cacgagactt attaaagatc ttattgccat
     3241 tttcacgtcc tgcccgacgg cgtcgctgtt cttctggcgg taacgcgctt tcttcgcagc
     3301 aaatctcact acaacaacct ttgtattttt ccgcacatac tggacactga ataaacagca
     3361 ggtggcagcc atcattttta cagttggtat ggctgtcgca cggcgcgccg cactggtggc
     3421 aatgcgcgat aatctcatcc gatatacgtt cgcccatccg ctcgtcaaaa acaaaatttt
     3481 tgccaataaa acgcaccggc aagccctgct cgcgcgcctt acgggcgtat tcaataattc
     3541 cgccctcgat atgccagact ttgttgaatc cgttatgttt catccaggca ctggcctttt
     3601 cacaacgaat gccgccggtg cagtacatga cgattttttt atctttatgt gcctgcatca
     3661 tctcgactgc ttttggcagc tgctcacgga aggtatctgc cggaatttcg agcgcgtttt
     3721 caaagtgccc cacttcatac tcatagtggt tacgcatgtc gataaatagt gcatcgggat
     3781 cgtcaagcat ggcgttcact tccgccgctt gcagatactc accaacattg ctggcatcaa
     3841 agtgaggatc gtcaataccg tcggcaacaa tgcgatcgcg taccttcatg cgcagtaccc
     3901 agaaagattt cccgtcatca tccaacgcga tattcaggcg taaaccctct aaagccgggt
     3961 cgaaggcata gagctgcgcg cgaaatgttt caacattgct cgcaggtacg ctgatttgcg
     4021 cgttaatgcc ctcatgcgcc agatacactc gcccaaaaac attcagcgcg gtaaacagct
     4081 gatataaagc gtcacgggtc gccttaggat cggcgatgtg gaaatacttg taaaacgaaa
     4141 tggtggttcg cggttcgctc tcagccaaca ttttggcttt tagcgcgtcg ttggaaatgc
     4201 ggttgtgtaa cactggcatg gtgtacggtt cctgcgagat gggaaagtaa aaatccgcgg
     4261 catgatatag caattatcga taattaacat ccacacattt tacgctacat ttgcgcatta
     4321 aaaattattt gttatttaca agcgcggcaa tttcgcccag tcttcagcca caaattttgg
     4381 ttgcgggcga aaaaatgcga caatacatac aattgcccga ataggttgaa aaacaggatt
     4441 gatatgacga atctacccaa gttctccacc gcactgcttc atccgcgtta ttggttaacc
     4501 tggttgggta ttggcgtact ttggttagtc gtgcaattgc cctacccggt tatctaccgc
     4561 ctcggttgtg gattaggaaa actggcgtta cgttttatga aacgacgcgc aaaaattgtg
     4621 catcgcaacc tggaactgtg cttcccggaa atgagcgaac aagaacgccg taaaatggtg
     4681 gtgaagaatt tcgaatccgt tggcatgggc ctgatggaaa ccggcatggc gtggttctgg
     4741 ccggaccgcc gaatcgcccg ctggacggaa gtgatcggca tggaacacat tcgtgacgtg
     4801 caggcgcaaa aacgcggcat cctgttagtt ggcatccatt ttctgacact ggagctgggt
     4861 gcgcggcagt ttggtatgca ggaaccgggg attggcgttt accgcccgaa cgataatcca
     4921 ctgattgact ggctacaaac ctggggccgt ttgcgctcaa ataaatcgat gctcgaccgc
     4981 aaagatttaa aaggcatgat taaagccctg aaaaaaggcg aagtggtctg gtacgcaccg
     5041 gatcatgatt acggcccgcg ctcaagcgtt ttcgtcccgt tatttgccgt tgagcaggct
     5101 gcgaccacga ccggaacctg gatgctggca cggatgtccg gcgcatgtct ggtgcccttc
     5161 gttccacgcc gtaagccaga tggcaaaggg tatcaattga ttatgctgcc gccagagtgt
     5221 tctccgccac tggatgatgc cgaaactacc gccgcgtgga tgaacaaagt ggtcgaaaaa
     5281 tgcatcatga tggcaccaga gcagtatatg tggttacacc gtcgctttaa aacacgcccg
     5341 gaaggcgttc cttcacgcta ttaaatctcc cctgccggat gcttcagaat ggcatccggc
     5401 attaccacag caaatccccc tgatttagcg ataaaagctc tctggattgc gccccctgga
     5461 agtcgggcgc ataattagtg tgcttatctt ttcttcttat gttcaccgcg cctggcgcac
     5521 caacagcgga ttgctatgtc accctgtgaa aatgacaccc ctataaactg gaaacgaaac
     5581 ctgatcgtcg cctggctagg ctgttttctt accggtgccg ccttcagtct ggtaatgccc
     5641 ttcttacccc tctacgttga gcagcttggc gttaccggtc actccgccct gaatatgtgg
     5701 tccggtattg tcttcagcat tacattttta ttttcggcca tcgcctcacc gttttggggt
     5761 ggactcgccg accgtaaagg ccgaaaactc atgctattac gctctgccct cggcatgggc
     5821 atcgtgatgg tgttgatggg actggcacaa aatatctggc agtttttgat cctacgggcg
     5881 cttcttgggt tacttggcgg atttgtcccc aacgctaatg ctcttatcgc cacacaagta
     5941 ccgcgtaata aaagcggctg ggcgctgggt acgctctcca caggcggcgt tagtggtgcg
     6001 ttgctcggcc caatggctgg cggcctgctc gccgatagct acggcttacg tccggtattc
     6061 tttattaccg ccagtgtgct catactctgc tttttcgtca ccctgttctg catcagagaa
     6121 aaattccagc cggtcagcaa aaaagagatg ctgcacatgc gggaagtggt gacatcactt
     6181 aaaaacccga aactggtact cagcctgttt gtcaccacgt taatcatcca ggtggcgaca
     6241 ggctcaattg cccccattct gacgctgtat gtccgcgaac tggcgggtaa cgtcagtaac
     6301 gtcgccttta tcagtggcat gatcgcctcg gtgccaggcg tggcggctct gctaagcgca
     6361 ccacgactcg gcaaacttgg cgatcgaatc ggacccgaaa agatcctgat tacagcgctg
     6421 atcttttctg tactgctgtt gatcccaatg tcttacgttc agacgccatt gcaacttggg
     6481 attttacgtt ttttgctcgg tgccgccgat ggtgcactac tccccgccgt acagacactg
     6541 ttggtttaca actcgagcaa ccagatcgcc gggcgtatct tcagctataa ccaatcgttt
     6601 cgtgatattg gcaacgttac cggaccattg atgggagcag cgatttcagc gaactacggt
     6661 ttcagagcgg tatttctcgt caccgctggc gtagtgttat tcaacgcagt ctattcatgg
     6721 aacagtctac gtcgtcgtcg aataccccag gtatcgaact gatttttcgc ctttcatact
     6781 tgcaaaagcg gagaatcagc tatccttttc cctgaaacct catcaactca aagggagaat
     6841 cgtgatgacc atgtacgcaa cgcttgaaga agccattgac gctgcacgcg aagaatttct
     6901 tgcagacaac cccggcatcg acgctgaaga tgcgaatgtg caacagttca atgcccaaaa
     6961 atacgttttg caggacggcg acatcatgtg gcaagttgag ttttttgccg acgaagggga
     7021 agaaggtgaa tgtttaccta tgcttagcgg tgaagccgcg caaagtgttt ttgatggcga
     7081 ctatgatgag atagagatac gccaggagtg gcaggaagag aatacattac atgaatggga
     7141 cgagggggaa tttcagcttg agccaccgct ggataccgag gaaggacgcg cagcagctga
     7201 tgagtgggat gaacgttaat cactcatacg ggccatgatg aatgtcgatc ggcagcagta
     7261 aagtatcaaa aaccagagag aatggcagat caaggatcgt gacataacgc caggcggagt
     7321 cacgcacatc ccattgcaca ccgggataat attggttgcc atgcccctgc cccggtatag
     7381 tgcgactaat aatgctgcca cagccactca gcaaggtcac catgatgctc accacaatta
     7441 aacgcatttg atacctcttt cgtaaaaaaa taccggcata acgccggtat ttttattggc
     7501 tcgttgtttt tgccggatgc ggcgtgaacg ccttatccga cccacaaaat cttgcaaaat
     7561 caataaattg caggaacgat gtaggcctga taagcgtagc gcatcaggca actacgtttt
     7621 tattgcgaag ccgcatccgg tttacgcaat gccagtggat tgagctttat cccttcgtaa
     7681 taactcaccc atgaagaata tctctccggg gaattccaga cgcggaaatg cagacgggcc
     7741 atcgtcaccg gatcgcttag cagcaccagg cgacgatcgc gattcagctt ctctggcgtc
     7801 tcgttcagcg cctgttcaac gtggcggtca cgggcgattt ccagcacctt actggcgcgg
     7861 tgacgcgcgg tcgccattgc ggttgccaga gcgttaaatg acgggttaaa cactgcgtgc
     7921 ataaagccat catcaaggga gcgttgacga ttcatctcaa ggaaccgatc ggtatcaacc
     7981 agcacctgcg gcggcgaata ctcttccggg atcaggaaca gtttccagcg tttggtgcgc
     8041 agaccaacgg tggcacggct ggaaatcacc gaaacaaacg gtgacaggat caacgagaag
     8101 acaatcggtg ccagccagaa caggaaacgc agatccaacc acgccatacc aacagcccac
     8161 actaacccta acagcagctg tgagccgtgg cgtttgaacg cttcacccca ggaagtggag
     8221 tcatcatcac gctgcggtga attccacacc acttcccagc caaggaacgc gctgacgacg
     8281 aagaccgtat ggaacagcat gcgtaccgga gccagcagca cggaaaacag cacttccagc
     8341 agcaacgata atgtaacgcg ccagaagccg ccgtattctt tcgttccttt gcaccagata
     8401 agcaaaatgc tcaataactt cggcaggaac aacagcacca tggtcgaagc aaaaagtgca
     8461 atcgccagct caggacgcca ctgcggccac accgggaaca actgccgtgg ttgcaggaag
     8521 tattgcggtt cggtcaacgc atgtactacc tgcaatgcag tagagagcgc gaggaacata
     8581 aaccacagcg gagcggagag ataagacatc acgcccgtca ggaataccgc acggtgaacc
     8641 gggtgcatac ccttcaccag gaacagacgg aagttcatca ggttaccgtg gcaccagcgg
     8701 cggtcacgtt ttagctcatc aagcaagtta ggcggcaatt cttcataaga acccgggaga
     8761 tcgtaagcaa tccagacccc ccaacctgca cggcgcatca acgccgcttc cacgaagtca
     8821 tgtgacagga ttgaaccggc aaaggaacct tcgcccggca gcggagccag tgcgcagtgc
     8881 tcgataaacg gtttcacgcg gataatcgcg ttatgtcccc agtagtgcga ctcgccaagt
     8941 tgccagaagt gcaaaccggc tgtaaacagt ggcccataca cgcgggtcgc gaactgctga
     9001 cagcgcgcat acagcgtatc cataccggac gctttcggcg acgactgaat gatcccggcg
     9061 ttcgggttgg cttccatcag gcgcaccagc ccgcacaaac aatcaccggt cattaccgag
     9121 tcagcatcca gcaccaccat gtagctgtac tggctgcccc agcgacggca gaagtcatcg
     9181 atattaccgc ttttacgctt cacgcggcga cggcggcggc gatagaaaat ctgaccttcg
     9241 ccaccgactt cagcgataag ctccatccag gctttttgct ctgcgacgca gatatccggg
     9301 ttataactgt cactaagaat gtagacatca aagtgtttgg cattcccggt ggcttttact
     9361 gattcccacg ttgcacgcag gccagcaaaa acacggttca cgtcttcgtt acagataggc
     9421 atgatcaacg ccgtgcgatg ctccgggttt aatggttcat cgccaactgt tgacgcagat
     9481 atactgtatt tatcgcgacc aataagcagt tgcaggaagc ccattaacgc cgtccagaat
     9541 ccggcggaca cccaacagaa cagtaccgca aagaggatca ggataccggt ttgcagcata
     9601 taaggcagaa gctgcataaa ggaaacccac aaatcctgac caaccatatc cataggatta
     9661 atcagcgccc aaccctgata aggaagaatg gtcttcatat accaggtcgc gacgacagtt
     9721 tgcgcgagcg tcaggatcaa cagaatgtaa cggcggatgg taccgacggt acgccacttt
     9781 tgctcgctct cctgctcttc tttggtcaaa cgagccagat agcgcggcgt gacatcgcgt
     9841 ccacgcaggc gatcccagaa acggcctacc gggttggtac gccacgggtc gggaaacatc
     9901 gaggagcgtt ttgcttctgg catcgccttc agctgatcgc gcccttcgtc gtctttaatt
     9961 aactgtccat cagcaagtga atctggccag gcttgttcca gacgcgcctt taccgagcct
    10021 tgcggggaat catcctcccg cgcccaggtg cggtgttcgg catccagcgc ctgatgaacg
    10081 gcgcggatat cagtcttcgg caatgccgct ttctcgcttg cggcgatggg cattgcgtca
    10141 atgtactcag ttgtcttatt cattggcagg taactggtag ctccaggttt cactcaacgt
    10201 ctgatcggca ttcaccagcg cagcacgcat ttcagtggtt ttcttggcat ctttcacttt
    10261 cacacgcatc accagacgcc agcctttggt aaccgggtta tagcgcaccg tgctttcaac
    10321 tatctcacca ttatcaccaa tgctggtttg cgctgtgacc ggggtatcct ctggcagttt
    10381 tttcatctct gcgccggtaa aatcgaccac aaaggcgata gtaccgtcag gctggcgaat
    10441 caggttcgac tgcttcacat cccccgttga acgacgcgtt tgttgcaccc atgcgttatc
    10501 tggcgcatgc agtttgtctt catcacggct gaaggtgatg gtgtatttaa agttcatctc
    10561 tttacccggc tccggcagct gatccggcgt ccagtaagcg acgatgttat cgttggtttc
    10621 atcgttggtt ggaatttcca ccagctcaac gctgccttta ccccactccc ctttcggagt
    10681 cacccatgcg cttggacgaa gatcgtaacg atcatcgaga tcttcaaagc gggagaaatc
    10741 acgaccgcgc tgcaacagac cgaagccttg cgggttctcc atcgagaagc tgctgaccgc
    10801 taaatgtttc gggttattca acggacgcca gatccattcg ccattaccgg catggataga
    10861 cagaccgtta gagtcgtgca actccggacg atagttattt gcaggcgacg gttggttcgg
    10921 cccaaacagg aacatactgg ttaacggtgc aacccccagt ttgccgactt tatcgcgcag
    10981 atagattttc gactgcacat ccacaaccgt gtcacgccct ggcataacca cgaatttgta
    11041 agcacctgtc gcgcgcggcg agtcaagcaa tgcataaatg gttaaacgtt tatcagtcgg
    11101 ttttggacgc tcgatccaga actctttgaa gcgtggaaat tcttcacccg atggcaaggc
    11161 ggtatcaatt gccaggccgc gggcagaaag gccataaacc tgacctgcac caatcacgcg
    11221 gaaatagctg gccccgagca tgctgacgat ttcatcgttt ttatctttgc tgttgatcgg
    11281 gtaaagcact ttaaaaccgg caaaaccaag gtcttttacc gtgtctttgt catgctgaac
    11341 atcgccgaaa gtgaaataat ccgggctgta tttgattcgt ttgactgcgg tggcagtcac
    11401 ttcatttatt ttgaccgggg tatcgaagta cataccctga tggtagaact cgagtttgaa
    11461 tggggtcttc agattgttcc agtacgcttt gtcatgatta aactggatct gctgatagtc
    11521 cgcgtatttc atatcgcgga aaacggaggg caagttgctt ttgggcgcct catagccttt
    11581 cccggctaag gattgagctt gctttgcgac atcatcaata ctgaaagccc agcttgaaga
    11641 tgtatacagg gttaacatta ctgcagcact caaccaacgc attttcatca tttgtagttt
    11701 atgtttcata attagtaagc acttccccct ttgtgtgctt atatcgatcc gatctatttt
    11761 aatggataac cagccattcc gacaacagaa tcccggtatc gttcagcacg ctggctcatg
    11821 gattaagcac ctgattgcaa ccgttttcac tttccgtgct tctcctaaag acagaagcat
    11881 cgacttcgtg tagggtttgc cagcaatttt atttatcaac cgtcttacag acgcttccat
    11941 tctggttagt ggtagcgatg atgctatgca taaaaacctt gaagcctgcg cctggcgcgg
    12001 tcaggtcggt gtcgctatgg caacaagcct aaaggcataa cccgacaggg ataagacgaa
    12061 aaatccgaga ttagttaacc atatatgaat ccagtacccg cgcaacgtga atatttcctc
    12121 gactccatcc gcgcctggct gatgttgtta gggatccctt ttcatatttc tttaatctat
    12181 tcgagccata catggcatgt gaatagcgcc gaaccatcat tgtggctgac cctttttaat
    12241 gacttcatcc actcgttccg catgcaggta tttttcgtta tatccggcta cttttcctac
    12301 atgctttttt tacgctatcc cttgaaaaaa tggtggaaag tacgtgtcga acgtgtaggt
    12361 atcccgatgt taacagccat ccccctactg acattaccgc aatttattat gctgcaatat
    12421 gtcaaaggaa aagcggaaag ttggcctggg ctgtcattgt atgacaaata taatacgttg
    12481 gcctgggaat taatatcaca cctgtggttt ttactggtgt tagtagtcat gacgacgctg
    12541 tgcgtatgga tatttaagcg catcagaaat aatttagaaa attctgataa aacgaataaa
    12601 aaattctcga tggtaaaact atcggtgatt tttttatgcc tcggcatcgg ttatgcggta
    12661 ataagaagaa cgatttttat tgtgtatccg cccattctga gtaatggcat gttcaatttt
    12721 attgtcatgc aaacgctgtt ttatttgccg ttctttatcc tcggcgcact ggctttcatt
    12781 ttccctcatc ttaaagcctt gtttaccacg ccgtctcgtg gctgtaccct tgcagcagca
    12841 ttggcgtttg tcgcttattt actcaaccag cgctatggca gtggcgatgc ctggatgtac
    12901 gaaaccgagt cggtgatcac catggtcctc ggtctgtgga tggtgaatgt ggtcttctcc
    12961 tttggccacc gtttgcttaa cttccagtca gcgcgggtga cttattttgt taacgcatcg
    13021 ctgtttatct atctggttca ccacccgtta acgctgtttt tcggcgcata cattacaccg
    13081 cacatcacct ccaactggct tggttttctc tgtggcctga tatttgtagt agggattgcg
    13141 ataattctgt atgaaattca tttgcgcatc ccgttactga agtttttgtt ctctggtaaa
    13201 ccggttgtta agcgtgagaa cgataaagca ccagcccgtt aagccacatt tacaataacc
    13261 attccacggg caatatcgac gccagtctga ccataacccg cttccagaaa ctggtggcgg
    13321 gttctttttt gagaataatc tctttctttg catgacgatc aacccagttg atccgtcccc
    13381 acctgtccag acggagctgc caggccgcat catactggct ctgaataaag cgtttatcaa
    13441 ttaactgtgc cagcgtttcg ctctctatca cgaagcccat ttcagtattg agcaatgttg
    13501 aacgcggatc gaaattgaaa gagccgataa acaccgtttt accatcgatg ctaaaggttt
    13561 tagcatgcag gctggctccg gaattaccgg ttatgccgcg atcgtgtaac gtactacttt
    13621 gttcacgcgt cggcttgagt tcatataatt ccacgccata gcggagcaat tttttgcgcc
    13681 agcgcgcgta tccggcatgg acgacagcaa catcgttagc ggcaagagaa ttggttagga
    13741 tcgcaatctt tacccctttt ctcaccatcc gtaagagttg cgccacacct gcgcgtgtcg
    13801 gtacaaaata ggaagagata atatcgatgc gttcactggg tgagcccatg atatcgaaca
    13861 ggcgctgcgg taacagtgaa tgccgttttg ccttgccctc ccctttcgcc ggatcatcac
    13921 ttaataaacg tgtcttcgcc cagataagcg gcaatgttcc atcaaccaga tgatttataa
    13981 atggactgga ttccattttg cgtaaataac gatgcgtcat ggcatcgtta tgccaggagg
    14041 cgggtaactc gatgcgatcc gccatttcac cctccgggac atccagcacc tgctgtaagg
    14101 gtgaaaccga tttgcaatac cagtagcggg cgaaatcatc ggcaacgtcc tctaccacgg
    14161 gtcctattgc catgacatct aaatccgaaa aaagtggctc ctcccctgct ccaaaatagg
    14221 catcaccaat atttcgtcct cccaccaggg tcaccacgcc atcgacagtg aaacttttat
    14281 tgtgcatacg gcgattaaga cgggaaaagt cggtgatata accaagcgga cgcagcaagc
    14341 gaaacgagaa aggattaaaa agccggactt caatgcgtgg atgactgtca agcaagcgta
    14401 aaatgtcgtc aagtccgggc gtattgttgt cgtccagcaa caaacggacg cgaacgccac
    14461 gctttgctgc ggctaacagg gcggaaaaca gtaaccgacc cgacatatcg tcctgccaga
    14521 tgtaatactg aacatcgagc gtatgctctg ccatttcggc cagacgatac cgggcggcaa
    14581 acgcatccag acttttctcc agaggaaaaa ggccacactg accgggatgt tgcgaacaca
    14641 gtggcagcac cgcgctcgcc agccggggca aatcattcat ctccttgttg ggtaaggagt
    14701 ctttcgtaga ggtgggcgtt ttcttcatca taacagacaa agtatacctg ttcgggtaaa
    14761 gcgtgacggg taataaattc tgaaacggtt tttactgcga tttcagccgc tgccgcacga
    14821 gggtaaccat aaaccccagt actgattgca ggaaaagcca ctgacgtata gctgtttgcc
    14881 gccaccagtc gtaggctatt gagataggca tcctgcaaaa gctggtcttc gttttgttca
    14941 ccaccacgcc agactggccc gacggtgtgc actacggctt tagcgggaag agcgcctgca
    15001 agcgtaataa cggcatgccc cgtagggcaa tcgccctgct gttgcctgac ttttaaacag
    15061 gcatccagta gggccggacc cgctgcgcga tgaatggccc catcgacgcc gccacctccc
    15121 attaatgaag gattagccgc attcacaatc acatcaacgg ccagtttggt aatatcaccc
    15181 tgcacaacat gaatacgcgt tttcataacg cctccttaca ccttcttgtt tcttaagtgt
    15241 acagcaggct ttttggttaa atcgcatttt tctactcagc aacgtgagtt gttcagaaac
    15301 ttagttatat tcgatgttca ttacagccag ctttttctgt ttatcaagat actgcacttt
    15361 tactgaggct atttgcgcgt ttttgacatt gccgctcatt aacgctttgc tggaataggt
    15421 tttgcccgga acagaaccat tctgtgggca gttcatttct acgggtgatg actgggtgct
    15481 gacgtcacat gccggttcca caatttggcc ataaaaatgg atcacgcccc cttgcgcggt
    15541 gcttcctgcg atggcaataa aaggaaaaaa caaactgccg agcataagag agtttaaaaa
    15601 aggtctgaac atttattcta tttcctcaat gattagtcat ccttgagggt tgtgttatcc
    15661 atactttcag gttggctgat tcgcagcaga ccattctctc cagattcatc ttatgctcga
    15721 tatttcaaca aattaagact tttctgaaga gggcggccat tgttgtgata aatgaagtga
    15781 ctgtccatca gaaacagtaa caactatttt cacccgatcg tccggggaaa tatttaaact
    15841 caacttcgtc aaagcaatgg gttgattagc aggcaatgaa agggtctttt cttgcttcgt
    15901 ctgactttgc cctgaactgc cttcgcgcag ggacaatatt tgtactctgc acagacaaga
    15961 ttgagtaaga gtgacttcag gaataatggt atacacatcc ccttgctggg tcgtattaaa
    16021 ggttatctga ctggaaagtg ccgcaaggag taataacgca ttcatattct tctcccgaaa
    16081 aaaaacaggg cttgcgccct gtttctgtaa tacaaatgat gtattagtac tgatgagcgg
    16141 tcgcgttgtt accaaagcca acctgagtca cgttgacgga ggagttagat gcagtctggt
    16201 caactgcagc accgttgcca ccaccgaact gtttaaccgt catttcagaa tttttgccgt
    16261 tccactgatc aagagtagcg ctgttaccga agccacgttg ggtcagatcg attgagctgt
    16321 catccgagcc ctgaccaaca tctgcaccat taccgccgcc atgctgggta atagtcaagt
    16381 cagagttacg ggcatcagtt tgcagagcaa gtgcagagtt accgccaccg tactggtaaa
    16441 tgttcagctc agaatttggg ccgctattat taccgccacc accgtggtta ccgccgccgc
    16501 cgtactgagg aacaacacct gccagagcgc taccggagaa tacgattgct gcaattgctg
    16561 ctacttttaa aagtttcatg taaaaccccc atcggattga tttaaaagtc gaatggaaat
    16621 taacgttgtg tcacgcgaat agccatttgc gactgtctct gcactacaat tgccgttttt
    16681 tgagtaccat actgtgtaat atttgcttta ttaccagaac ctttctggat aatcatcgca
    16741 gtattaccat aagcaccttg cgaaatactg gcatcgttgg cactgcccgc ctgatcaata
    16801 tatgcaaggt tataatctcc tgtctggtca atctttgccc ggttgctact accttcttgc
    16861 gcaacaaccg ccaaaagttt tgagcctccc tgccgtaact gagcactatt attcgtccca
    16921 gcttgaccaa ttatggctgc ctgattaaat gaagacttac tcaattcatt taccgcgaag
    16981 ttatattctg aattagctaa atcataacct gctgcggctg caatcccagg cgcacccagt
    17041 attgttaaca tcataaataa caatttgttt ttcatgttgt caccctggac ctggtcgtac
    17101 atttaagaaa ttaaatcatt tcaacttggt tgttaacgca acctgtattt tgttaacgct
    17161 gcgttacgat ggaaagtatg tctgcggaaa tatttttaat aactcacccg cgcgttgtat
    17221 ttttcttttt ttgttcatac caaagtatta aaacccacgt aaatacgctg ttatctacgc
    17281 aaaaaatatt ttgttttctt ttaaatctcc gttttccgct atcaaaaatc accagacagt
    17341 catttttctt gcccgtcgct gattgctgcg atatgtcttg cgcacaagcc gtgacaaacc
    17401 tcgtcttt
//
LOCUS       DAJOWB010000099        17369 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJOWB010000099 DAJOWB010000000
VERSION     DAJOWB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.099"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            108..569
                     /locus_tag="OHO85_003535"
     CDS             108..569
                     /locus_tag="OHO85_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegJ family protein"
                     /protein_id="HCQ2672859.1"
                     /translation="MKKILLMLSLLFFTTAGFSEVSDTLVTGGYDKQAMSDAIKHARK
                     ETDKFIEVMNKKDADTFAVKAPITDHGRTEHFWLTDVTYSNGMFIGVISNDPGIVTNV
                     EYGQEWKIKKEDISDWMYTRGDKIYGGYTIDPLLVTYPKEEADELRAKLVR"
     gene            complement(634..1395)
                     /gene="pphC"
                     /locus_tag="OHO85_003536"
     CDS             complement(634..1395)
                     /gene="pphC"
                     /locus_tag="OHO85_003536"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCQ2672860.1"
                     /translation="MSWRLVYASTVGTSHISADLPCQDACQMQIAWLNDQQPLLSVFV
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATNMVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMAGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLAAATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWAE"
     gene            complement(1392..2051)
                     /locus_tag="OHO85_003537"
     CDS             complement(1392..2051)
                     /locus_tag="OHO85_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCQ2672861.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMNGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(2272..2331)
                     /gene="ibsA"
                     /locus_tag="OHO85_003538"
     CDS             complement(2272..2331)
                     /gene="ibsA"
                     /locus_tag="OHO85_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCQ2672862.1"
                     /translation="MMKHVIILLILLVISFQAY"
     gene            complement(2604..2660)
                     /locus_tag="OHO85_003539"
     CDS             complement(2604..2660)
                     /locus_tag="OHO85_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCQ2672863.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            2939..4186
                     /gene="mdtA"
                     /locus_tag="OHO85_003540"
     CDS             2939..4186
                     /gene="mdtA"
                     /locus_tag="OHO85_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCQ2672864.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLIALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            4186..7308
                     /gene="mdtB"
                     /locus_tag="OHO85_003541"
     CDS             4186..7308
                     /gene="mdtB"
                     /locus_tag="OHO85_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCQ2672865.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            7309..10386
                     /gene="mdtC"
                     /locus_tag="OHO85_003542"
     CDS             7309..10386
                     /gene="mdtC"
                     /locus_tag="OHO85_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCQ2672866.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAVSNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            10387..11802
                     /locus_tag="OHO85_003543"
     CDS             10387..11802
                     /locus_tag="OHO85_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2672867.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            11799..13202
                     /gene="baeS"
                     /locus_tag="OHO85_003544"
     CDS             11799..13202
                     /gene="baeS"
                     /locus_tag="OHO85_003544"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCQ2672868.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            13199..13921
                     /gene="baeR"
                     /locus_tag="OHO85_003545"
     CDS             13199..13921
                     /gene="baeR"
                     /locus_tag="OHO85_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCQ2672869.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            14112..14444
                     /locus_tag="OHO85_003546"
     CDS             14112..14444
                     /locus_tag="OHO85_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCQ2672870.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            14653..14949
                     /locus_tag="OHO85_003547"
     CDS             14653..14949
                     /locus_tag="OHO85_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HCQ2672871.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            14951..15247
                     /locus_tag="OHO85_003548"
     CDS             14951..15247
                     /locus_tag="OHO85_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCQ2672872.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            15350..16711
                     /gene="yegQ"
                     /locus_tag="OHO85_003549"
     CDS             15350..16711
                     /gene="yegQ"
                     /locus_tag="OHO85_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCQ2672873.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(17042..17359)
                     /locus_tag="OHO85_003550"
     CDS             complement(17042..17359)
                     /locus_tag="OHO85_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672874.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         4052 a         4589 c         4602 g         4126 t
ORIGIN      
        1 aaaatgataa tgcaatttta tcaggataca attcaatttc ggcctgataa tgattaactt
       61 ccacaggttt ttgctaactt tgactacaaa gggattctgg agaaaaaatg aaaaagatat
      121 tacttatgtt gagtctgcta ttttttacta ccgcgggctt tagtgaagtt tcggatacgt
      181 tagtgacggg aggttatgat aaacaagcga tgtctgacgc gattaaacat gcgcggaaag
      241 aaaccgataa attcatcgag gttatgaata aaaaagatgc cgatactttc gcagtcaaag
      301 ccccgataac cgatcacgga agaaccgagc atttctggct tacggatgtc acttattcta
      361 acggcatgtt tattggcgta atcagcaatg accctggtat tgttacaaac gtagaatatg
      421 gccaggagtg gaaaatcaag aaagaagata tttcagactg gatgtatacg cgcggggata
      481 aaatttacgg cggttatacc attgatcctt tattggtcac ctatccaaaa gaagaagccg
      541 acgagctaag agcaaaatta gttcgctaat cagccgttat atttattgtt taaactggcg
      601 gttgaattgt tgtcttcaac cgccatgaga aactcactct gcccacagcg ccagtgccaa
      661 cgttttatca tcatccgtac gctcgtttac cgccgggctg gacaaaaact gctttaacaa
      721 ttcaggtaat aaatcgagtt gttcctgcgt tgctgccgcc agtccattga aaaacggggt
      781 aaagaaaggc acatgaggag aattatccag catatttaac gccagtcgct ggataccatc
      841 cgtaaatgct gcaactttgt gcgcacgccc agtgctggtg aaagtttcca gtctggaaac
      901 ggcatcttcg tcggtaatga agtgcgtcat gttggcatat tcacccgcca tcggcgtgag
      961 aggcaattgc agaccgtgac ccaaatctac caccacgccg ccatcgccaa tctgcataat
     1021 caaggtgccg tcaggcgatg aaatcagtcc cagaaatgtg caggcaaaat cgcgaaccgc
     1081 taattctttg gcttccgcct cggcaaaaag tcgttgacga atggtcagta ccatatttgt
     1141 ggcgaggaca tcattaagcc ccaattcccc gccctgcact ttttgcgaca tataagccat
     1201 cgcttcattg acggcgagca tcgcgccttc gccaccctgc gagacactgc ctgcgccatc
     1261 agcgacaaat accgacaata atggctgctg gtcatttaac caggcaatct gcatctggca
     1321 ggcatcctga caaggcaagt ccgcgctgat gtgcgacgtt ccaacggttg atgcatagac
     1381 cagacgccag ctcacactga cgtccagcct tttggcgctt ccagcacaac ttccgttccc
     1441 ggcgtggagc gggaaaccga acgcagcgaa ctggataacc agctaaacag ttcacggaat
     1501 tgtaatcctt gcagaggcaa aggctgacga acgctaattt gtgccagcgt cttcatatcc
     1561 gccccctgaa caccgatgga aaagaaagca aaccttttat cttcttcccc ccgaaacact
     1621 ttgttggctg cagcctgcca ctcatcggtt ggtgcaccat cggtaatcag gaaaatccac
     1681 gggcgataat acgaaatacc atttgcacga tactcacgtt tccgctcctc taccatatcc
     1741 agggctttgg taatggccgc acccattggt gtatcgccct gggcaaacag gatgggcgga
     1801 aaaaaattag cggcgctggt aaagggttgt tccacatgca ccgggccgaa cgtcacaatg
     1861 ccgagttcca ctctttttaa agccagcgga tcagcaagca gttcatcacg aaaggtaacc
     1921 aatccggcgt taagttcatt aataggtcta ccgttcatag aaccagaaac atccagcagc
     1981 aaaatgcagg ggcaacgtgg ttccgggtta ctggcaaaat cgctggtggc aaacgtgatt
     2041 tgttcgctca taagaaatcc tttcttagag aattatcgag gtccggtatc ataccgactg
     2101 caataatgaa ataagagcaa acgaagaaac tttcagaatt aggcccggcg gcgcagaaaa
     2161 ttttgctatt cgcctcgcaa gaacggagag ccgtcgattg acactaaggg cggagtgaca
     2221 taatttcagg agtgagggtt agggagaggt ttccccctcc ccctggtgtt cttagtaagc
     2281 ctggaagcta atcactaaga gtatcaacag tatgatgacg tgcttcatca taaccctttc
     2341 cttattaaaa gccctcttct ccgggagagg ctttcccgtt tcagcgtccc gctgaaatca
     2401 tcggcttacc tcctttcgcc ctgaatgcag tctatcgcta acgcgtgaat aaaatagttt
     2461 cctctgtgat tattgcgtaa cgactcgcaa agaatatttg ctggcaggat cgcagactac
     2521 aaagcctgcg gattgacaat cttatcgtga aggcatactt tcaggagtga gggtagagcg
     2581 gggtttcccc cgccctggta gtcttagtaa gcggggaagc ttatgactaa gagcaccacg
     2641 atgatgagta gcttcatcat gaccctttcc ttatttatgg ccccttcttc gggaggggct
     2701 ttcccgtttc agcgtcccgc tgaaatcgtc ggcttacctc ctttcgccat gcaagcagtc
     2761 tatcgctaac gcgtagataa aatagtttcc tgtgttatta ctggatgcgt gctcgcaaat
     2821 gtgcccgtca ttcagacgat tccagacagt gtttcataat tcctccattt ttctccctta
     2881 ttggctggct acactagtat cattccgcga aacgtttcag gaagagaaac tcttaacgat
     2941 gaaaggtagt tataaatccc gttgggtaat cgtaatcgtg gtggttatcg ccgccatcgc
     3001 cgcattctgg ttctggcaag gccgcaatga ctcccggagt gcagccccag gggcgacgaa
     3061 acaagcgcag caatcgccag cgggtggtcg acgtggtatg cgttccggcc cattagcccc
     3121 ggttcaggcg gcgaccgccg tagaacaggc agttccgcgt tacctcaccg ggcttggcac
     3181 cattaccgcc gctaataccg ttacggtgcg cagccgcgtg gacggccaac tgatagcgtt
     3241 acatttccag gaaggccagc aggtcaaagc aggcgattta ctggcagaaa ttgaccccag
     3301 ccagttcaaa gttgcattag cacaagccca gggccaactg gcaaaagata aagccacgct
     3361 tgccaacgcc cgccgtgacc tggcgcgtta tcaacaactg gcaaaaacca atctcgtttc
     3421 ccgccaggag ctggatgccc aacaggcgct ggtcagtgaa accgaaggca ccattaaggc
     3481 tgatgaagca agcgttgcca gcgcgcagct gcaactcgac tggagccgga ttaccgcacc
     3541 agtcgatggt cgcgttggtc tcaagcaggt tgatgttggt aaccaaatct ccagtggtga
     3601 taccaccggg atcgtggtga tcacccagac gcatcctatc gatttagtct ttaccctgcc
     3661 ggaaagcgat atcgctaccg tagtgcaggc gcagaaagcc ggaaaaccgc tggtggtaga
     3721 agcctgggat cgcaccaact cgaagaaatt aagtgaaggc acgctgttaa gtctagataa
     3781 ccaaatcgat gccactaccg gtacgattaa agtgaaagca cgctttaata atcaggatga
     3841 tgcgctgttt cccaatcagt ttgttaacgc gcgcatgtta gtcgacaccg aacaaaacgc
     3901 cgtagtgatc ccaacagccg ccctgcaaat gggcaatgaa ggccattttg tctgggtgct
     3961 gaatagcgaa aacaaggtca gcaaacatct ggtgacgccg ggcattcagg acagtcagaa
     4021 agtggtgatc cgtgcaggta tttctgcggg cgatcgcgtg gtgacagacg gcattgatcg
     4081 cctgaccgaa ggggcgaaag tggaagtggt ggaagcccag agcgccacta ctccggaaga
     4141 gaaagccacc agccgcgaat acgcgaaaaa aggagcacgc tcctgatgca ggtgttaccc
     4201 ccgagcagca caggcggccc gtcgcgcctg tttattatgc gtcctgtggc caccacgctg
     4261 ctgatggtgg cgatcttact cgccgggatt atcggttatc gcgccctgcc cgtttcggcg
     4321 ctgccggaag tggactatcc gaccattcag gtggtcacgc tctacccagg tgccagcccg
     4381 gatgtcatga cctctgccgt taccgcgccg ctagaacgcc agttcgggca gatgtctggc
     4441 ctgaaacaga tgtcgtcgca aagttccggc ggtgcgtcag ttatcacttt gcagttccag
     4501 ctaacattac cgctcgatgt cgccgagcag gaagtgcagg ccgcgattaa cgctgcgacc
     4561 aacttgttgc cgagcgatct gcctaacccg ccggtttaca gcaaagtgaa cccggcagat
     4621 ccgccgatca tgacgctcgc cgtcacctca accgccatgc cgatgacgca agtggaagat
     4681 atggtggaaa cccgcgtcgc gcagaaaatc tcgcagattt ccggcgtcgg cctggtgacg
     4741 ctttccggcg gtcagcgtcc ggctgttcgc gtcaaactta acgctcaggc gattgccgcc
     4801 ctcggcctga ccagcgaaac cgtgcgcacc gccattaccg gcgctaacgt taactcggca
     4861 aaaggtagcc tcgacggccc ttcccgtgcg gtcacgcttt ccgcgaacga ccagatgcaa
     4921 tccgccgaag agtatcgcca gctaatcatc gcctaccaga acggcgcgcc aattcgtctg
     4981 ggcgatgtcg caactgtaga gcaaggtgca gaaaacagct ggctcggcgc gtgggcgaac
     5041 aaagaacagg ccattgtgat gaatgttcag cgccagcccg gtgctaacat tatctccacc
     5101 gccgacagca ttcggcagat gctgccacag ctcactgaga gtctgccgaa atcggtgaag
     5161 gtgacagtgc tttccgatcg caccaccaat atccgcgcat ccgtcgatga tactcagttt
     5221 gaattgatga tggctatcgc gctggtagtc atgattatct acctgttttt gcgcaatatt
     5281 ccggcgacca tcattcccgg tgttgctgta ccgctgtcgt taatcggcac tttcgcggtt
     5341 atggtgtttc tcgatttttc aatcaataac ctgacactga tggcgttaac tatcgccacc
     5401 ggattcgtgg tcgatgacgc catcgtggtg atcgaaaaca tttcccgcta tatcgaaaaa
     5461 ggcgaaaaac cgttggcggc agcgctcaag ggcgcaggtg aaatcggctt taccattatc
     5521 tcgctgacct tctcactgat tgcggtgttg atcccactgc tgtttatggg cgatatcgtc
     5581 gggcgactgt tccgcgaatt tgctattacc ctggcggtag cgattttgat ctcagcggtg
     5641 gtgtcgctga ccctgacacc gatgatgtgc gcgcggatgc tcagccagga gtcgttgcgt
     5701 aaacagaacc gcttctcccg tgcctcggaa aaaatgttcg acaggataat cgccgcctat
     5761 ggtcgtggac tggcgaaagt gctgaatcat ccgtggctga ccttaagcgt ggcactcagc
     5821 acgctgctgc ttagcgtgct gctgtgggtg ttcattccga aaggtttctt cccggtacag
     5881 gacaatggca ttattcaggg cactttgcag gcaccgcaat ccagctcctt tgccaatatg
     5941 gcccagcgac aacgccaggt cgcggacgtg attttgcagg atccggcagt gcaaagcctg
     6001 acctcatttg ttggcgttga tggcactaac ccgtcgctga acagtgcacg tttacaaatc
     6061 aacctcaaac cgttggatga acgtgatgat cgggtgcaaa aagtcatcgc ccgtctgcaa
     6121 acggcggtag ataaagtgcc gggcgtcgat ctcttcctgc aaccaacgca ggatctgact
     6181 attgatactc aggtcagccg cacccagtac cagtttacct tgcaggccac gtcactggat
     6241 gcgctcagta cctgggtgcc acagttgatg gaaaaactcc agcaactgcc acagctttct
     6301 gatgtctcca gcgactggca ggacaaaggg ctggtggcgt atgtcaatgt tgatcgcgac
     6361 agcgccagcc gtctggggat cagcatggcg gatgtcgata acgccctgta caacgcgttt
     6421 ggtcagcggc tgatttccac tatttatact caggccaacc agtatcgcgt ggtgctggag
     6481 cacaacaccg aaaatacccc aggcctcgcg gcgctggata ccattcgcct gaccagcagc
     6541 gacggcggcg tggtgccgct aagctcaatt gccaaaattg agcagcgttt tgcgccgctc
     6601 tccatcaacc atctggatca gttcccggta acgaccatct cctttaacgt gccggataac
     6661 tattcgctgg gcgatgcggt gcaggcgatt atggacaccg aaaagacgct gaatctgccg
     6721 gtggatatca ccacgcagtt ccagggcagc accctcgcct tccagtcggc gctgggcagc
     6781 actgtctggc tgattgtcgc ggcggtggtg gcgatgtata tcgtgctcgg cattctgtac
     6841 gagagcttta ttcacccgat caccattctc tcgacgctac ccaccgcagg ggttggcgca
     6901 ctgctggcgt tgctgattgc tggtagcgaa ctggatgtga ttgcgattat cggcattatt
     6961 ttgctgatcg gtatcgtgaa gaagaacgcc atcatgatga tcgacttcgc gctggctgcc
     7021 gagcgcgagc aaggcatgtc gccgcgcgag gcaatctacc aggcttgtct gttgcgtttt
     7081 cgtccgatcc tgatgaccac cctggcggct ctgcttggcg cgctgccgct gatgttgagt
     7141 accggggtcg gcgcggaact gcgtcgtccg ttaggtatcg gcatggtcgg cggcctgatt
     7201 gtcagccagg tgctgacgct gttcaccacg ccggtgattt atctgctgtt cgaccgcctg
     7261 gcattgtgga ccaaaagccg ctttgcccgt catgaagagg aggcgtaagt gaagtttttt
     7321 gccctcttca tttaccgtcc ggtggcgacg attttactgt cggttgccat taccctgtgc
     7381 ggcatactgg gcttccgtat gctgccggtc gccccgctgc cgcaggtcga ttttccggtg
     7441 attatggtca gcgcctcgct gcccggtgcg tcaccagaaa caatggcgtc ttccgttgcc
     7501 acgccgctgg agcgctcact tgggcgcatt gccggagtca gtgaaatgac ctccagcagt
     7561 tcgctcggca gcacgcgtat tattttgcag tttgattttg accgggatat caacggcgca
     7621 gcgcgtgatg tgcaggcggc gatcaacgct gcacaaagtt tgctgcccag tgggatgccc
     7681 agccgcccga cctatcgcaa agcgaacccg tcggatgcgc caattatgat cctcacgctg
     7741 acgtccgata cttattcgca gggtgaactg tacgatttcg cctcgacgca gctggctccg
     7801 acgatttcgc aaatcgacgg tgttggtgat gtcgatgtcg gaggcagctc actgcccgcc
     7861 gtacgcgtcg ggctgaatcc gcaggcgctg tttaatcagg gcgtgtcgct ggacgacgta
     7921 cgcaccgccg tcagcaatgc caacgtgcgt aaaccgcagg gcgcgctgga agatggcact
     7981 caccgctggc agatccagac caatgatgag ctaaaaaccg ccgctgaata tcagccgttg
     8041 attattcact acaacaacgg cggcgcggtt cgtctgggcg atgtggcgac ggtgaccgac
     8101 tcagtgcagg atgtgcgcaa cgccgggatg accaacgcca aaccggctat tttactgatg
     8161 atccgcaaac tgccggaagc caatattatc cagacggttg acagcatccg ggcaaaatta
     8221 ccggagttgc aggaaaccat tccggcggcg attgatctgc aaattgccca ggatcgctcc
     8281 cccaccattc gcgcctcgct ggaagaagtc gagcaaacgc tgattatctc ggtggcgctg
     8341 gtgattctgg tggtgttttt attcctgcgc tcgggtcgcg ccactattat tcccgccgtt
     8401 tcggtgccgg tttcgctgat tggtacgttt gcggcgatgt acctgtgcgg attcagtctc
     8461 aataaccttt cgttaatggc gctcaccatc gctactggtt tcgtggtgga tgacgccatc
     8521 gtggtgctgg aaaacattgc acgtcatctg gaagcgggaa tgaaaccgtt gcaagccgca
     8581 ctgcaaggta ctcgcgaagt cggttttacg gtgctgtcga tgagtctgtc actggtggcg
     8641 gtgttcctgc cgctgctgtt gatgggcgga ttgccgggcc gactgttacg cgaatttgcc
     8701 gtgacgcttt ctgtcgccat tggtatatcg ttgctggttt ctctgacatt aacgccaatg
     8761 atgtgtggct ggatgctgaa agccagcaag ccgcgcgagc aaaagcgact gcgtggtttt
     8821 ggtcgcatgt tggtagccct gcaacaaggc tacggcaagt cactgaaatg ggtgctcaat
     8881 catacccgtc tggtgggcgt ggtgctgctt ggcaccattg cgctgaatat ctggctgtat
     8941 atctcgatcc cgaaaacctt cttcccggag caggacactg gcgtgttgat gggcgggatt
     9001 caggcggatc agagtatttc gtttcaggcg atgcgcggta agttgcagga tttcatgaaa
     9061 attatccgtg acgatccggc agtggataat gtcaccggct ttacaggcgg ttcgcgagtg
     9121 aacagcggga tgatgtttat caccctcaag ccacgcgacg aacgcagcga aacggcgcag
     9181 caaattatcg accgtctgcg cgtaaaactg gcgaaagaac cgggggcgaa tctgttcctg
     9241 atggcggtac aggatattcg cgttggtggg cgtcagtcga acgccagcta ccagtacacg
     9301 ttgttatccg acgacctggc ggcactgcga gaatgggagc cgaaaatccg caaaaaactg
     9361 gcgacgttgc cggaactggc ggacgtgaac tccgatcagc aggataacgg cgcggagatg
     9421 aatctggttt acgaccgcga caccatggca cggctgggaa tcgacgtaca agccgccaac
     9481 agtctgttaa ataacgcctt cggtcagcgg caaatctcga ccatttacca gccgatgaac
     9541 cagtataaag tggtgatgga agtggatccg cgctataccc aggacatcag tgcgctggaa
     9601 aaaatgttcg ttatcaataa cgaaggcaaa gcgatcccgc tgtcgtattt cgctaaatgg
     9661 caaccggcga atgccccact atcggtgaat catcagggat tatcggcggc ctcgaccatt
     9721 tcgtttaacc tgccgaccgg aaaatcgctc tcggacgcca gtgcggcgat cgatcgcgca
     9781 atgacccagc ttggtgtgcc ttcgacggtg cgcggcagtt ttgccggcac ggcgcaggtg
     9841 ttccaggaga cgatgaactc gcaggtgatc ctgattatcg ccgccatcgc cacggtgtat
     9901 atcgtgctgg gtatccttta cgagagttac gtacatccgc tgacgattct ctccaccctg
     9961 ccctcggcgg gcgttggagc gctgttggcg ctggagctgt tcaatgcccc gttcagccta
    10021 atcgccctga tagggatcat gctattaatc ggcatcgtga agaaaaacgc cattatgatg
    10081 gtcgattttg cgcttgaagc ccaacggcac ggtaacctga cgccgcagga agctattttc
    10141 caggcctgtc tgctgcgttt tcgcccgatt atgatgacta ccctggcggc gctgtttggt
    10201 gcgctgccgc tggtattgtc gggcggcgac ggctcggagc tgcggcaccc cctggggatc
    10261 accattgtcg gcggactggt aatgagccag ctccttacgc tgtataccac gccggtggtg
    10321 tatctctttt tcgaccgtct gcggctgcgt ttttcgcgta aacctaaaca aacggtaacc
    10381 gagtaaatga cagatcttcc cgacagcacc cgttggcaat tgtggattgt ggctttcggc
    10441 ttctttatgc agtcgctgga caccaccatc gtaaacaccg cccttccctc aatggcgcaa
    10501 agcctcgggg aaagtccgtt gcatatgcac atggtcattg tctcttatgt gctgaccgtg
    10561 gcggtgatgc tgcccgccag cggctggctg gcggacaaag tcggcgtgcg caatattttc
    10621 tttaccgcca tcgtgctgtt tactctcggt tcactgtttt gcgcgctttc cggcacgctg
    10681 aacgaactgt tgctggcacg cgcgttacag ggcgttggcg gcgcgatgat ggtgccggtc
    10741 ggcagattga cggtgatgaa aatcgtaccg cgcgagcaat atatggcggc gatgaccttt
    10801 gtcacgttac ccggtcaggt cggtccgctg ctcggtccgg cgctcggcgg tctgctggtg
    10861 gagtacgcat cgtggcactg gatctttttg atcaacattc cggtggggat tatcggtgcg
    10921 atcgccacat tgctgttaat gccaaactac accatgcaga cgcggcgctt tgatctctcc
    10981 ggatttttat tgctggcggt tggcatggcg gtattaaccc tggcgctgga cggcagtaaa
    11041 ggtacaggtt tatcgccgct gacgattgca ggcctggtcg cagttggcgt ggtggcactg
    11101 gtgctttatc tgctacacgc cagaaataac aaccgtgccc tgttcagtct gaaactgttc
    11161 cgtactcgta ccttttcgct gggcctggcg gggagctttg ccggacgtat tggcagtggc
    11221 atgttgccct ttatgacacc ggttttcctg caaattggcc tcggtttctc gccgtttcat
    11281 gccggactga tgatgatccc gatggtgctt ggcagcatgg gaatgaagcg aattgtggta
    11341 caggtggtga atcgctttgg ttatcgtcgg gtactggtag cgaccacgct gggtctgtcg
    11401 ctggtcaccc tgttgtttat gactaccgcc ctgctgggct ggtactacgt tttgccgttc
    11461 gtcctgtttt tacaagggat ggtcaactcg acgcgtttct cctccatgaa caccctgacg
    11521 ctgaaagatc tcccggacaa tctggcgagc agcggcaaca gcctgctgtc gatgattatg
    11581 caattgtcga tgagtatcgg cgtcactatc gccgggctgt tgctgggact ttttggttca
    11641 cagcatgtca gcgtcgacag cggcaccaca caaaccgtct ttatgtacac ctggcttagc
    11701 atggcgttga tcatcgccct tccggcgttc atctttgcca gagtgccgaa cgatacgcat
    11761 caaaatgtag ctatttcgcg gcgaaaaagg agcgcgcaat gaagttctgg cgacccggta
    11821 ttaccggcaa actgtttctg gcgattttcg ccacctgcat tgtcttgctg atcagtatgc
    11881 actgggcggt gcgtatcagt tttgagcgtg gctttattga ttacatcaag catggtaatg
    11941 aacagcgatt acaactgtta agtgatgcgc ttggcgagca gtatgcgcag catggcaact
    12001 ggcgcttcct gcgcaacaat gatcgctttg tctttcagat cctgcgttca tttgaacacg
    12061 ataattcgga agataaaccc ggcccgggta tgccaccgca cggctggcgt acccagttct
    12121 gggtggttga tcaaaacaac aaagtgctgg ttggtccgcg agcgccgatt ccacctgacg
    12181 gtacacggcg acccattctg gtcaacggtg cggaagttgg cgcggtgatc gcctcccccg
    12241 ttgagcggtt aacgcgcaat actgatatca atttcgataa acaacagcgg caaaccagct
    12301 ggttgattgt cgccctggca acgttactcg cggcacttgc cacttttctg ctggcgcgcg
    12361 gtttactggc accggtaaaa cgacttgtcg atggcacgca caaactggcg gcgggcgatt
    12421 tcactacccg cgtaacgccc accagtgaag atgaactggg caaactggcg caagacttca
    12481 accagcttgc cagcacactg gagaaaaacc agcaaatgcg gcgcgatttt atggccgata
    12541 tttctcacga actgcgtacg ccattagcgg tgctgcgcgg tgaactggaa gccattcagg
    12601 atggcgtgcg taaattcacg ccggagacgg tggcgtcttt acaggcggag gtcggtacac
    12661 tgaccaaact ggttgacgat ctccatcagt tgtcgatgtc tgatgaaggc gctctcgcct
    12721 atcaaaaagc accggtagat ttgatcccac tgctggaagt ggcgggcggc gcatttcgcg
    12781 aacgattcgc cagtcgtggc ctgaaactgc aattttccct gccagacagt attaccgtat
    12841 ttggcgatcg cgaccgttta atgcagttat tcaataactt actggaaaac agcctgcgct
    12901 acactgacag cggcggcagc ctgcaaatct ctgccgggca gcgcgacaaa acggtgcgcc
    12961 tgacctttgc cgacagtgcg ccaggtgtca gtgacgatca gctacaaaaa ttgtttgaac
    13021 gtttttatcg caccgaaggt tcccgcaacc gtgccagcgg cggttccggg ctggggctgg
    13081 cgatttgcct gaacattgtt gaagcacata atggtcgcat tattgctgcc cattcgcctt
    13141 ttggcggggt aagcattaca gtagagttac cgctggaacg ggatttacag agagaagtat
    13201 gaccgagtta ccaatcgacg aaaacacacc gcgtattttg atcgtggaag atgaaccgaa
    13261 gctggggcag ttgctcattg attatctgcg tgctgcgagc tatgcgccga cgcttatcag
    13321 ccacggcgat caggtactgc cgtatgtgcg ccagacacca ccggatctga tcctgttaga
    13381 tctgatgctc cctggcaccg atggcctgac gctgtgccgg gaaattcgtc gtttttctga
    13441 cattccgatc gtgatggtga cggcaaaaat cgaagagatc gatcgcctgc tggggctgga
    13501 gattggcgca gatgattata tctgtaagcc gtacagccca cgggaagtgg tagcgcgcgt
    13561 caaaaccatt ttgcgccgtt gcaaaccgca gcgcgagttg cagcaacagg atgctgaaag
    13621 cccgttgatt atcgacgaag gtcgttttca ggcttcatgg cgcggtaaaa tgcttgacct
    13681 gacgcctgcg gaatttcgtc tgctgaaaac gctctctcac gaaccaggaa aagtgttctc
    13741 ccgcgagcaa ttgctcaatc atctttatga cgactaccgc gtagtaaccg accgcaccat
    13801 cgacagccac attaaaaacc tgcgccgcaa gctggaatct ctcgacgccg aacagtcatt
    13861 tatccgcgcc gtttatggcg tcggttaccg ctgggaagcc gacgcctgcc gcatcgttta
    13921 gttttagcga cattattttg ttagccggag atggcgttca gaccagatcc ggcaacatta
    13981 tcccacgcat ggtcagcaaa ctgacattat ctctccgccc tggcaggcat cagaaaatcg
    14041 ctcatacttt aatcggtaaa cagcaccttt agatgctgtt ttcgatacgc aatttcaatc
    14101 aaggagtcat tatggctggt tggtttgaac tcagtaagag cagtgataat cagttccggt
    14161 ttgtgctaaa agcgggcaat ggtgagacta tcctcaccag cgagctgtat acctcaaaag
    14221 cctctgcgga aaagggcatc gcgtcggtgc gtagcaacag cccgcaagaa gaacgctatg
    14281 agaaaaaaac ggcaagtaac ggcaaattct atttcaatct gaaagccgct aatcatcaaa
    14341 ttatcggctc cagccagatg tacgccaccg cgcaatctcg tgaaaccgga attgcctccg
    14401 ttaaagccaa tggcacaagc cagacggtga aagacaatac gtaattacga tgccgggcgt
    14461 gggttgttgg cgtccggctt tatatccaaa taacgcttag ttattaataa ttaagagtaa
    14521 ccgccgccat taaaatagtg ggcactgacg agcactcgtt gtgctttctt tgtttaccat
    14581 cccatactta tcctcctgaa catgttcatg tgccaaaata tcttctaata ctttcgtgac
    14641 gctatccggt ttatgacata cactgatgta agatttttac ttacaggcgg gcgtaaaatg
    14701 gccagaacga tgacggttga tctcggcgat gagttgcgtg aatttatcga gtccttaatt
    14761 gaatccggcg actatcgcac ccaaagcgaa gtgatccgtg agtcactgcg attacttcga
    14821 gaaaagcaag cagagtctcg cctgcaagcg ttacgtgaca tgctcgcaga agggttgagt
    14881 agtggtgaag ctcagccgtg ggaaaaggac gcgttcttac ggaaggtgaa agcagggata
    14941 aggaaataat gtgcgtatta ttaaactcat gccaaaagcc aatgaggatt tagaaggcat
    15001 ctggtattac agctatcacc atttcggcga accacaagcc gacagatatg tggaacatct
    15061 ttcagacgtt ctccagattc tgagtaacaa taatataggt acgcccagac ctgaattagg
    15121 cgaaggtatt tttgttcttc cttttgaacg ccacgtcatt tatttcctgc aatcaccagg
    15181 ggaaattatc gtcattcgta tccttaacca aaaccaggac gccaccagac atttacactg
    15241 gagctaaacc cgccgattgc gctacaatgc ccgcccttaa agtgggggca ctcccctaac
    15301 cgcttcatca ggtgaagcgg atctgacctg tcatcagaac gagagaatta tgtttaaacc
    15361 ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt tacgctttcg cttatggcgc
    15421 agatgctgtt tatgcgggcc agccgcgtta ttccctgcgt gtgcgcaaca acgaattcaa
    15481 ccacgaaaat cttcagctcg gcatcaatga agcccacgcg ctggggaaaa agttttatgt
    15541 cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc tttatccgtg acctgaaacc
    15601 ggtggtggaa atggggccgg atgcgctgat tatgtccgat ccagggctga ttatgctggt
    15661 gcgtgagcac ttccctgaaa tgccgatcca cctttcggtg caggctaacg ccgtgaactg
    15721 ggcgacggtg aaattctggc agcaaatggg cctgacccgc gtgatcctct ctcgcgagct
    15781 gtcgctggaa gagattgaag agatccgcaa tcaggtgccg gatatggaga tcgagatctt
    15841 cgttcacggc gcgctgtgca tggcctactc cggtcgctgc ctgctctctg gctatatcaa
    15901 caagcgcgac ccgaaccagg gcacctgcac caacgcctgc cgctgggagt acaacgtcca
    15961 ggaagggaaa gaagatgatg ttggcaacat cgtacacaag tacgagccga ttccggtgca
    16021 aaatgttgag ccgacgctgg gtatcggcgc accaaccgac aaagtgttta tgatcgaaga
    16081 ggcccagcgt ccgggcgagt atatgaccgc gtttgaagat gagcacggca cttacatcat
    16141 gaactcgaaa gatctgcgcg ccatcgccca tgtagaacgc ctgaccaaaa tgggcgtgca
    16201 ttcgctgaaa atcgaaggtc gtaccaaatc tttctactat tgtgcacgca ccgcacaggt
    16261 ttaccgcaaa gctatcgatg acgccgctgc gggaaaaccg ttcgatacca gcctgctgga
    16321 aactctggaa ggtctggcgc atcgtggcta taccgaaggt ttcctgcgtc gtcatactca
    16381 cgacgattat cagaactacg aatacggtta ttcagtttct gaccgccagc agtttgttgg
    16441 tgagtttacc ggtgagcgca agggggacct cgcggcggta gcggtgaaaa ataaattctc
    16501 cgttggcgac agccttgagc tgatgacgcc gcaaggcaac attaatttta cccttgagca
    16561 catggaaaac gccaaaggcg aagctatgcc gatagcacca ggcgatggtt atactgtgtg
    16621 gctcccggtc ccgcaggatc ttgagctcaa ttacgcgctg ctgatgcgta atttctccgg
    16681 ggaaaccacg cgtaatcccc acggtaagtg attaatttcg attatttttc ccggatggaa
    16741 aattcttaga aaccgatcac atacagctgc atttattaag gttatcatcc gtttcgctga
    16801 aaaacataac ccataaaatg ctagctgtac caggaaccac ctccttagcc tgtgtaatct
    16861 cccttacacg ggcttatttt tttatgcata agccctatct ctggtaaccg tcttccattg
    16921 accacatcga tagaatctcc cttcatagca cgatgccttt cacgtaacgg catcgtgctc
    16981 gcacaggttc cggctaagca caaccagaac gcgcatgttt gacgcttacc aaaaaatatt
    17041 ctcactctcc acatttgaat gtcagacgag cgacgccatg taatcctgca ccttctgtct
    17101 tcaggtcaac tatctgcatt tttttgccct gagtaacaca gaaatgggct gcatcatttt
    17161 ttactatatt ttctgcacca gatattctgc ccctggctaa agaagcttcg gcttcggtgt
    17221 agtattgatt atcgagttta cgctgaatat tacttttata tgcaagacca aatttaccga
    17281 tacttgtctc atcattatgc acagcacaac cagacataat aaaaatacta attaatgata
    17341 tagcagctat ctttttcatc tcaccttcc
//
LOCUS       DAJOWB010000100        17301 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJOWB010000100 DAJOWB010000000
VERSION     DAJOWB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.100"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..2457
                     /locus_tag="OHO85_003551"
     CDS             <1..2457
                     /locus_tag="OHO85_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pcar"
                     /protein_id="HCQ2672875.1"
                     /translation="ETCLTELSKLHNEEVVTEHELRSEILRPYISEKTAPRLHIMTGT
                     GGQYDFDDVIQNENTFLSLLSNQIISIKPSGYNITITTIDKKTAQVMKEEYQERIAII
                     QKKLNNDVSRLEENIKKNRCEIQNASSYDLAFFINKMGRSGFERYIACCSTPEQHDGE
                     SITDNAANIDFIYFLLSHGYLSTDYMAYRSVFMPGSLSTEDNNFIRAVTSGRLPDETA
                     KMPLSNIANTVAKLHGLGILMHDNAWHPQILWYLMRNDTNSLKTIMRMQAEVGAERRM
                     VRLANEIFPLWEPAAQREYIRLMVDGDGHLSTMIHQIGRLNDTVAEQNLLPVLLSLPI
                     LSWEAVSQITREELQRLIDLQFNLVTSLPENCAQFFCENLRNSGCRLTNIPLARSDSG
                     QETLHLVVQKKLWTYSTLNLQNICFSLSHESENNSDTFRKKPVALIKSLRIPNLEKYV
                     YENISSFIRDVFIHSEENDLIPDFLNSTFVDWDDAKYMTESMSFVLEDVSVILNKENT
                     ETTEISYDQNLYSLLAHHNHITPCWNNVISLLSEDASIAGDTFCEWLNINYSLLPNDS
                     LPLTDVQFSQLLIKAVTSPHISKEALIAITMAFRITLINVPENLPLNNAAVLIKQKWL
                     APTSTVFEQLYQALYEEGDKLTSLLYALICARPVLLSDNYELVLFSDDQFDLGITRLI
                     LNGDKIADEVCISILNWLWEKDEALLSEAPLLSQQALIRFSTKITDDRQKQALLMQCL
                     KNDGGSHKFIRQVLMTFGHQDYAAFLTERNYRSIPRSDAMWQLAVQLGNSGFIRPPKL
                     THADTRIRIEPFFNAENEYD"
     gene            complement(2502..2918)
                     /locus_tag="OHO85_003552"
     CDS             complement(2502..2918)
                     /locus_tag="OHO85_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HCQ2672876.1"
                     /translation="MNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRAAVDATTEVKVALRLAGTPIGPN
                     DTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG"
     gene            complement(2915..3145)
                     /locus_tag="OHO85_003553"
     CDS             complement(2915..3145)
                     /locus_tag="OHO85_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCQ2672877.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLR
                     PSWSSFLELEKADPGFMTEREDVVCDEGRFNL"
     gene            3410..3910
                     /locus_tag="OHO85_003554"
     CDS             3410..3910
                     /locus_tag="OHO85_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672878.1"
                     /translation="MGNYSDFETDFVQRTLALIDQYNEMIEVLGKPFREQYNYTLTLN
                     CLLGLIVLPKERALSFLPADRLTRQLKAEMGLHESQLPGPEMNLRELIHKMRNSVAHF
                     CVQVESASDAHLVDWIVFRESQEDGEVYASFSAPELLPFLKYYATLLLDNMARRRAPD
                     VNILDL"
     gene            3923..4696
                     /locus_tag="OHO85_003555"
     CDS             3923..4696
                     /locus_tag="OHO85_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672879.1"
                     /translation="MLILDCSSRTQALHTLSAGFGCSPEKLKKVLLSLDLESIYELNP
                     RQLVDAPQYLREYVCAELGEPGPFTRALWFHGTRTFAGNTFPAGLLALNQSESLAMKM
                     LLDLAPNEMVRTHLKEWDVPGGVPDEMFQLRTGDKIHWGPFGHLVRELHFNASENGLH
                     DYLWLPELVEDVCKAYQKKYGHDLKPHYLSVLHPCIVWFEADIVYEKGVLETALSYAY
                     TSVRDLPPDGNATFGIDCDGKSVSRSAIARIEFLQPGQM"
     gene            4863..5996
                     /locus_tag="OHO85_003556"
     CDS             4863..5996
                     /locus_tag="OHO85_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HCQ2672880.1"
                     /translation="MHFYVDETGQTGRNLFDKTQPVLSYGVLSSDANLDKVAEADLAV
                     IRKTLGVQRLHAAELGLHRLSDLVDTLLVLQKKHRIRFDIWQVVKRDHAIISFFDQVF
                     DQGMNPAVPWSAYWTPLRYPLLLNLASLFDDELASNAWTARLEAHDERASELFCTVSD
                     ELISRTAASALDHRSKQLITDALNWASANFEQLGYNCKTNKERLRIMPNMIGFQSVLH
                     GICSRLGAPERKASIIVDQQSQFNTTQRELNEFYYQIRDMPWELGPGLPVMNMKNMPA
                     EPLVFQSGTKSAGLELVDIYLWTFKRFMEDKALTKPLSRLVYTNLKTARTNSVSIQSV
                     ASRFKELLGKLPVPSAEIMRQAQELRDFDEARRMPYVVSGSPD"
     gene            complement(6030..6542)
                     /locus_tag="OHO85_003557"
     CDS             complement(6030..6542)
                     /locus_tag="OHO85_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672881.1"
                     /translation="MTIFKENIVDTSLIGPILTALLAAAGFYCKFWFTRYQASRDQAQ
                     KVSRAIEDVLSKMAALFGQKKPARILREEFSAVIAPLHQEMRILDDMTSRLPLKWLQR
                     EQRHVLYHARWLQRYLDSRRGGSDGDFFLLLHDVAMGTDYQVADILASQHDCKQRRGS
                     QLPVNLKTCP"
     gene            7108..7326
                     /gene="ccdA"
                     /locus_tag="OHO85_003558"
     CDS             7108..7326
                     /gene="ccdA"
                     /locus_tag="OHO85_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCQ2672882.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERW
                     QAENQEGMAEVARFIEMNGSFADENRDW"
     gene            7328..7633
                     /gene="ccdB"
                     /locus_tag="OHO85_003559"
     CDS             7328..7633
                     /gene="ccdB"
                     /locus_tag="OHO85_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCQ2672883.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            7634..8440
                     /locus_tag="OHO85_003560"
     CDS             7634..8440
                     /locus_tag="OHO85_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HCQ2672884.1"
                     /translation="MSGSVIHSQSAAMVPAVYSAGQSAQLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNKR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPITPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPESDAVVMLKQLS"
     gene            complement(9120..9851)
                     /locus_tag="OHO85_003561"
     CDS             complement(9120..9851)
                     /locus_tag="OHO85_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCQ2672885.1"
                     /translation="MGELKTTHTIMTMTISTAKKSVNISQSNELTEAAYYLPLQAKRV
                     LWLCLIQCYPLKDDPDSVSPVFTVLVADYQKYFKVSVDTASSDVKKGVTALAESSVVF
                     YPKDGEFDEIKRPWLAEAGLKKGRGKWQIEFNYKVMPYLMGLTSQFTTYSLYDCGKIN
                     SVRAIRLYESLCQYRSSGVWVTTQEWLSERFMLPESQRSNFAEMKRTFINPALKKINA
                     NTPLKAAMTQNEDGRLVFTIVDSKN"
     gene            10472..11677
                     /locus_tag="OHO85_003562"
     CDS             10472..11677
                     /locus_tag="OHO85_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCQ2672886.1"
                     /translation="MRRDYGSVGTIALRASALLQAMSRDIEAQRKEFNLTQYHQTYTR
                     NAVAKLPKLSRRIVELAVKEMEDSGYEFNKKQVGNVEQYALTIQNVIDIYAHRQIPKY
                     RDIHKEPYVIFVVNLKGGVSKTVTTVTLAHALRVHPDLLRHDLRILVIDLDPQASSTM
                     FLDHTHSIGTVLETAAQAMLNDLDAETLREAVIRPTIIPGVDIIPASIDDGFVASQWE
                     SLVTEHLPGLKPSEVLKKTIIDRIAGDYDFVFIDTGPHLDPFLLNGLAASDLLLTPTP
                     PAQVDFHSTLKYLTRLPEMLERLEEEGVEPRLSASIGFMSKMTGKRDHETSHSLAREV
                     YASNILDASLPRLDGFERCGESFDTIISANPVSYPGSAEALKKARTEAERFTKAVFDR
                     IEYIRGASK"
     gene            11674..12645
                     /locus_tag="OHO85_003563"
     CDS             11674..12645
                     /locus_tag="OHO85_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCQ2672887.1"
                     /translation="MRKVLARGRVLGNSNSEFARMLESDGDVKTFTLKSGKQAKFVKT
                     VVLSGEIESKTFVDSSVNGRDQAMLSPESVSDISRTIKLQQFFPAIGREVNGRIEILD
                     GTRRRAACIYNNVKFEILVTKDDIELSDARQLAKDIQTAREHSLRELGKRLEVTYGTS
                     MTKEEIAQAENLSPAKVTRAFQAAAVPDEMVAVFPVINDISLSDYHFLLKLAEEANNK
                     QRSVTELMEKVQHRLKTMPDYPAIDKSKILAVIRAESKSLIVRPTRTVQTEKLREFSD
                     RNQFARKKIDSKKRLVVYEFSRISAEAQSEIDEAIKRILERLPESSE"
     gene            complement(12782..13927)
                     /gene="umuC"
                     /locus_tag="OHO85_003564"
                     /pseudo
     CDS             complement(12782..13927)
                     /gene="umuC"
                     /locus_tag="OHO85_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490544.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
     gene            13990..14988
                     /locus_tag="OHO85_003565"
     CDS             13990..14988
                     /locus_tag="OHO85_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCQ2672888.1"
                     /translation="MHKKLLDALPFLSADPSACRWVCLQEDLLCAPNTIDAYARGVND
                     WLAFCHSVALTAAVAGRDTVALYVRSLHTGRQLAAATIRHRLTVVRLYNDWLCEEGIR
                     ERNPVRRGVWKNGGKGKAGIVPLQRRLPWIPDDAEWLRFLEVAGQADIRTRFMLALAY
                     DCGLRREELCTVATGDIDPSQRLLTVRAEHTKNRFGRVVPYSPVTGELYTAWLTERRM
                     LSSSRGPLFLSRSPRNRAEPISNWTWSKVVRGLAIKADLPLISTHTFRHLCLTELARV
                     GWDIHEIAAFAGHRRIQSTLLYIHLSARDLSSRFNYTVASLHTCRLTVLQQQDPSP"
     gene            14985..15896
                     /locus_tag="OHO85_003566"
     CDS             14985..15896
                     /locus_tag="OHO85_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672889.1"
                     /translation="MMQSEHTAPCPTTSLSLPALLWDTRPEISESELAALDTLVDHFQ
                     QGGKNWSPDIQKRLSRLLLPLRDTLTKMHAAKAPYNSSIHDIVLEMQRIRKTYWAWTQ
                     EEWLEVICNSEGEFRRRFGARGNCRQYVIALAWLLCGFERLEHCGIFYQYRLCLKVFG
                     RQSTDFAVSQLDNMMQVLGYVPRDSRNNGIRNAMCMAMLLQRDAQLDHITVTTLQQIA
                     ATCPDYLREASATLSRILAASGTIEEGFDYRITQRRRPPREYNATADVPTKWLVWCKR
                     WRATSVLRPSSILSGWYVLLKCGQLVS"
     gene            15973..17196
                     /locus_tag="OHO85_003567"
     CDS             15973..17196
                     /locus_tag="OHO85_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2672890.1"
                     /translation="MKVGEWANPGGMYRDRRGKMMMPAARARMLGRIRTFFHDLQEWG
                     WIPVRFSPERVFRAPRSLTSLVGPEPRIVADDMWCKLLHAGQNLQESDLPNCVAYPYF
                     YPLEMVRALSVLWLFGGLRRDEILRMQCGCIRWQQPEENNVSRICLIDVPVSKTYAAF
                     TKPVDPIIGEYIEQWELVCNPHPLQEDSKTGESVRFLFMHRGKRVHSSYINNSLIPLL
                     CRKAGIPEQDARGKITSHRARATIASQLYNAREPLDIFELQKWLGHSSPESTRHYVEI
                     TPTRLAGSLDKAGYFERNRRMVSVLIDQDAIAEGLVASGKPWRYYDLGHGFCTYDFFE
                     QCPHRMACAKCSFYMPKSSSEAQYLEGRQNLLKLMQEIPLTDDEQAAVENDIQAVDKL
                     LNKLTGGAPPGNKRK"
BASE COUNT         4443 a         4131 c         4194 g         4533 t
ORIGIN      
        1 gaaacgtgtc taactgaact atctaaacta cataatgaag aagtggtcac tgaacatgag
       61 ttaagaagtg aaattttgcg gccatatatc agtgaaaaaa cagcaccccg attacatatt
      121 atgacaggta caggagggca atatgatttt gatgatgtaa tccaaaatga aaatacattt
      181 ctttctttgc tgagcaacca aataatcagc attaaaccta gtggatataa cattactata
      241 acaacaatcg ataaaaaaac agctcaggtt atgaaggagg aatatcagga aagaatagcg
      301 attattcaaa agaaactaaa taatgatgtt tctcgattgg aggaaaatat taaaaaaaac
      361 aggtgcgaga ttcagaatgc atcctcgtat gatcttgctt ttttcatcaa taaaatgggg
      421 agatcaggtt tcgaaagata tattgcatgt tgttcaacgc ctgaacaaca tgacggagaa
      481 tccattactg ataatgccgc aaatattgat tttatttatt tccttctatc tcatggttat
      541 ctttccactg actatatggc ctaccgctca gtctttatgc caggtagtct gagcacggaa
      601 gacaataact ttatcagggc tgtgacgtca ggaagattac ctgatgaaac ggcaaaaatg
      661 cccctcagca atattgctaa cacagtagca aaattgcatg gactgggcat actgatgcat
      721 gacaatgcat ggcatcctca gattctctgg tacctcatgc gcaatgatac gaactctctg
      781 aagactatca tgaggatgca ggcagaggtc ggagccgaac ggcggatggt aagactggcc
      841 aatgagatct tccccctatg ggaacctgct gcgcagcggg aatatatcag attaatggtc
      901 gacggggatg gtcatttatc cactatgatc caccagattg ggcgcctgaa tgataccgta
      961 gctgaacaaa atcttctccc ggtattactg tccttaccga tactttcttg ggaagccgtc
     1021 tctcagataa ctcgggaaga actgcagaga ctcatcgatt tgcaatttaa tctcgtaacg
     1081 tccttaccgg agaattgtgc gcagtttttc tgtgaaaacc tgcggaattc aggttgtcgc
     1141 ctgactaaca ttcctttggc ccggagtgac tctgggcagg aaacattgca tttggtggta
     1201 cagaaaaaat tgtggaccta ttccacgtta aacctgcaaa acatttgttt ctcactttct
     1261 catgagtcag agaataacag tgataccttc cgtaagaagc ctgttgcttt aataaaatcg
     1321 cttcgtattc cgaaccttga aaaatatgtt tatgaaaaca tatcatcatt cattcgtgat
     1381 gtttttatac actctgaaga aaatgatctc attcctgatt ttcttaattc gacattcgtt
     1441 gactgggatg atgcaaagta catgacagaa agtatgagct tcgtactgga agacgtatca
     1501 gttatcctga acaaagagaa taccgaaaca acagaaattt cttatgatca aaacctgtat
     1561 tcacttctgg ctcatcataa tcatattacc ccttgctgga ataatgttat ttctctactg
     1621 agtgaagatg cgtcaattgc tggtgatact ttttgtgagt ggcttaatat taattattct
     1681 ttattgccaa atgactcgtt gcctttgact gatgttcagt tctcgcaatt acttattaag
     1741 gctgttacat cacctcatat cagtaaagaa gcgttaatag cgataaccat ggcatttagg
     1801 ataactctta ttaatgtgcc agaaaactta ccactgaata atgctgcggt actaatcaag
     1861 caaaaatggc tggcaccaac cagtactgtg tttgaacaac tttatcaggc actgtacgaa
     1921 gaaggtgaca aactgacatc actactttat gcacttattt gtgcccgtcc tgtattattg
     1981 agtgataatt acgagcttgt tttattttct gatgaccagt ttgacctggg gattactcgt
     2041 ttaattctga atggcgataa gattgcagat gaagtctgta tcagtatcct gaactggctc
     2101 tgggaaaaag atgaagccct gctctctgag gcaccacttc tcagccagca agccctgata
     2161 aggttctcca ccaaaataac cgatgaccgg cagaagcagg cactgctgat gcaatgtctt
     2221 aaaaatgacg gtgggtcaca caagttcatc agacaggtgc tcatgacgtt cgggcatcag
     2281 gattatgccg ctttcctcac agagaggaat tatcgtagca ttccccgtag tgatgccatg
     2341 tggcaactgg ctgtccagct gggaaatagt ggatttatca ggccgccgaa acttactcac
     2401 gctgatacac gtattcgtat agagcccttt tttaacgctg aaaatgaata cgactgacat
     2461 cgcaaaatag tggtaagtcc gtcgggggcg gccttactcc tttatcctgc ccagtcttcc
     2521 agtaccagtc caggcacccg ctcaaactct ctcacattat tcgtcaccag tattgccccg
     2581 gtggcgatgg cgtgcccggc gatcgccgtg tcgtttggac cgatcggcgt gccggcgagg
     2641 cgcagcgcca ccttaacctc cgtggttgca tccaccgcag cgcgatccca gggcaggatg
     2701 gcatcgagac gggcgcagaa cgcgtcaacc agctgcacgt ggcgtggcga tgcctttgga
     2761 ccggtggcgc cgaagcgcat ctcggagtag gtgatggccg agaccacgat acggtgaccg
     2821 cgcagcaccg cctgctccag attcttaagc actgcttcag gctgctcgcg catgatgaac
     2881 gagcagatgt tcgtgtcgag catatagatc ttgttcacag gttaaatcgt ccttcgtcgc
     2941 agacaacgtc ctcgcgttcc gtcataaagc ccggatcggc cttttctagc tccaggaatg
     3001 agctccagct cgggcggagg ggacgcagaa tgattctgtc cccttcccgt acaatctcca
     3061 gctcactcac cccctcaaaa tccagatcgc ggggcagacg gatcgcgcgg ttattgccat
     3121 ttttgaaaat agatacggtt ctcatgtctt cctcctggca aggcggcttt tatatatgtt
     3181 tagtatatgc agatgtgata catatgtata tgttataaat atgcagatgt gtgagatatg
     3241 cgtatgcctg tcatatgcag atgccaaaca tatggatatg catggtatct gcatgtttct
     3301 ctcttttttc accgggatcc gaaaaatatc atagtaggaa atgccgtacc tgagccgtca
     3361 ggctggccgg atgatatagt ggattctcga accggaacgg gagggttaaa tgggaaatta
     3421 ttcagatttt gagaccgatt ttgttcagcg aacactggcg cttatcgatc aatataacga
     3481 aatgattgag gtgctgggca aaccattcag ggagcagtac aactacacgt tgaccctgaa
     3541 ttgcctcctg ggtcttatcg tgttaccgaa agagagagcg ctctctttcc tgcctgccga
     3601 ccgtttaacg cggcaactta aagctgaaat gggattacat gagtcacagc tgcccggacc
     3661 ggaaatgaac ctgcgggagt tgatccataa gatgcggaat tcagttgctc atttctgcgt
     3721 ccaggtagag tcagccagcg atgcacacct tgtagactgg attgttttca gggaatctca
     3781 ggaagatgga gaagtctacg ccagcttcag cgctccggag cttttaccct ttctgaaata
     3841 ttatgcgacc ctgctgctcg acaatatggc ccgccgtcga gcccccgatg ttaacatatt
     3901 ggatttgtaa gggatttaca ccatgttgat tctcgattgc tcctcacgaa ctcaggcgtt
     3961 gcatacatta tctgccgggt ttggctgttc ccctgaaaag ctgaaaaaag tgctgctttc
     4021 gctcgatctt gaaagtatct acgagcttaa tcctcgccag ttggttgatg cacctcagta
     4081 tctgagagaa tacgtctgtg cggaactggg cgaaccggga ccattcaccc gggctctgtg
     4141 gtttcatggc acccggactt tcgccggtaa tacttttccg gcggggttac tggcgctaaa
     4201 tcagtcagaa tccctcgcca tgaaaatgct gcttgacctg gccccgaatg agatggtccg
     4261 tacacacctg aaggaatggg atgttccggg gggcgttccg gatgagatgt ttcagctcag
     4321 aaccggcgat aaaatccact gggggccttt tggccattta gtccgggaat tacattttaa
     4381 cgccagtgaa aatggactgc atgattacct ctggctgccg gagctggtag aggatgtctg
     4441 taaggcctac cagaagaaat acggccacga tcttaaaccg cattatctga gcgtgcttca
     4501 tccctgtatt gtctggttcg aggcggatat tgtttatgaa aaaggagttc ttgaaacggc
     4561 tctgtcatat gcctatacat cagtccgtga cctcccgccg gacggaaacg caactttcgg
     4621 catagactgt gacgggaaat ctgtgtctag gtcggccatt gccagaatcg agtttctgca
     4681 gcccgggcag atgtaatccc taaaaaacgg gtctgtgatc cggcccgttt gttcattgcc
     4741 gtgaacccag tattgaggtc attaaagcct taagtggatc acttgcagct ggctgcagaa
     4801 aggttttaca ttgacccgga ttatgccgtt tcccttcctc ctgcctggta aaggatctct
     4861 gaatgcactt ttatgtggat gaaacaggcc agactggccg aaaccttttt gacaaaaccc
     4921 agccagtcct ctcctacgga gtgctgtcgt ctgatgctaa tctggataag gttgctgaag
     4981 cggatctagc cgtaatacgc aaaacgcttg gcgtgcagcg cctgcacgct gcggaactgg
     5041 gcttgcaccg cctttctgac cttgtagata cgctgctggt tctgcagaaa aagcaccgga
     5101 tacggtttga catctggcag gtagtaaagc gcgatcatgc cattatttcg ttcttcgacc
     5161 aggtatttga tcagggaatg aatccagccg taccctggtc tgcatactgg acccctctgc
     5221 gctaccccct gctgctgaat ctggccagcc tgtttgatga tgaacttgct tcaaacgcat
     5281 ggacggcaag gctggaggcg catgatgagc gtgcgtcgga acttttttgt accgtcagtg
     5341 atgaactgat cagcaggacg gcagccagcg cgctggacca ccggtcgaag cagctgatca
     5401 ccgatgccct gaactgggcg tcagccaatt ttgaacagtt gggctataac tgtaaaacca
     5461 acaaggaaag gctgcggatt atgcccaata tgattggctt tcagtccgta ttgcacggga
     5521 tctgttcccg actgggggca ccagaaagaa aggccagtat cattgttgac cagcagtcac
     5581 agttcaacac cacccagcgt gaacttaacg agttttacta ccagatacgg gatatgccct
     5641 gggaactggg gcccggatta cctgttatga acatgaaaaa tatgccggct gaaccacttg
     5701 tattccagtc cggtaccaag agtgcaggtc tggagttggt agatatttat ctctggacat
     5761 tcaagcgatt catggaagac aaggcgctga caaagccact ttcccgcctt gtttatacca
     5821 atctgaaaac agccaggacc aacagcgtgt caatccagtc ggttgccagt cgatttaagg
     5881 aactcctggg taaactcccg gtaccaagcg cagagatcat gaggcaggcg caggagctgc
     5941 gggactttga tgaagccaga cgcatgccat atgtggtgtc gggatcaccc gactgatgcc
     6001 ggatattatc tgctttcaac tgccccctct taggggcagg ttttcaggtt aaccggcagc
     6061 tgtgatccac gacgctgctt acagtcatgc tgcgatgcca gaatgtcagc aacctgataa
     6121 tcggtcccca tcgccacatc gtgcaatagc aggaaaaagt ccccgtcact gccgcccctg
     6181 cgcgaatcca gataccgctg cagccatcgg gcatgataaa gaacgtggcg ctgttcgcgc
     6241 tgcagccatt tcagcggcag acgggacgtc atatcgtcaa ggatacgcat ttcctgatga
     6301 agcggcgcaa taacagcgga aaattcctct ctcagtatcc gggcaggttt cttctgtccg
     6361 aacagcgcgg ccattttgct caggacatcc tcgatcgccc ggctgacttt ctgggcctga
     6421 tcacgggagg cctgataacg cgtaaaccaa aacttacagt agaaccccgc agcggcaagc
     6481 agcgccgtaa ggatcggtcc aatcagactt gtgtccacaa tattctcctt aaagatggtc
     6541 atgaggccac ctgaagcagc cgatgacgca aatatactcc gttttagcaa ttagtgctat
     6601 gcgagaatgc aaaaagtgct attcgttgtt tttggtgttg acattttaat ttatgcgtga
     6661 gttttttgtt ttctttcagt tttctccact gagatccgaa aaccccaagt tccggatccg
     6721 cggatccttc gctgccgccc gctggtccgc tttcacagga tgtggtgtcc cgctccgctg
     6781 acatgacact gtatgttgtg tttcttcccg tgttcccgag ccagtcatca gcggcagaaa
     6841 agatcgcttc gcttattcac tgatcctgat tcacttccgg aaaatcctga atccgataaa
     6901 aatcggatca tagaaaaaca gtcatcctgt aatttatcat ggttactaca ttgcaggtta
     6961 ttattgtcat gattactttt caggtcagtt attgatggtg gtgttaataa tagctggata
     7021 acaatattcc gttgccggag gcatgttata gcgtaatact ggtgtatact taatggtgta
     7081 cagagagtat gtaagatgag gtgtattatg aagcagcgca ttacagtcac cgtcgacagt
     7141 gacagctatc agttgctcaa ggcatatgat gtcaatatct ccgggctggt aagtaccacc
     7201 ctgcagaatg aagcccgtcg tctgcgtgcc gaacgctggc aggcagaaaa tcaggaaggg
     7261 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgccgacga gaacagggac
     7321 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
     7381 ggatgtccag agtgacatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag
     7441 tgcacgtctg ttgtcagata aggtctcccg tgagctttac ccggtggtgc atgtcgggga
     7501 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
     7561 ggaagtggct gatctcagcc accgagaaaa tgacatcaaa aatgccatta acctgatgtt
     7621 ctggggaata taaatgtcag gctccgttat acacagccag tctgcagcca tggtaccggc
     7681 agtgtattct gccggacagt ctgcacaact tcctgttgcc attgattatc cggcagctct
     7741 ggcactccgc cagatgtcga tggttcatga tgaactgccg aaatatctgc tggctcccga
     7801 ggtgagtgcc ctgcttcatt atgtcccgga tctgcgccgc aagatgctgc tggccactct
     7861 gtggaacacc ggcgcgcgta ttaatgaagc actagcgctg acgcgggggg atttttcgct
     7921 cacgcctccg tatccgtttg tgcagcttgc gaccctgaaa caacggaccg aaaaagccgc
     7981 caggacggca ggaagaatgc cagccggtca gcagactcac cggctggtcc cgctctccga
     8041 ctcctggtac gtcagccagc tgcagacgat ggtggcaaca ctgaaaatcc ccatggaacg
     8101 gcgtaataaa cgaacaggca ggacagagaa agcgcggatc tgggaagtga cggacagaac
     8161 ggtcaggacc tggattgggg aagcggttgc cgccgctgcc gctgatggtg tgacgttctc
     8221 tgtcccgatc acgccacata cgttccgcca ttcctatgcg atgcacatgc tgtatgccgg
     8281 tataccgctg aaggtcctgc agagcctgat ggggcataag tccatcagct caacggaggt
     8341 ctacacgaag gtatttgcgc tggatgtggc tgcacggcac cgggtgcagt ttgcgatgcc
     8401 ggaatctgat gcggttgtga tgctgaaaca attatcctga gaaaatgctc tggccagtat
     8461 atgggaaata ccccaggttt tcccgttgga tgggaaacag gcatgattag gatgagtcgc
     8521 cagactgaac cgccccggga atcctggaga ctaaactcgt tgagagagag gtaaacagaa
     8581 tctatgtatc gttccggtga actcgcattg ccgccagaca tcatagttgc cattctgcac
     8641 tttccctgta tctgagctgt ctcttataca caagtcccct cccggggatt tgtgatgtct
     8701 gtcagtctgc tttagtgggg actctttcca tccggtactt tccttcctcc ccggtagcca
     8761 ttttttgaac tttttcttca cccgctgatc ttctcttata tttcattttt ttcgggtctg
     8821 ttatggtact ttctgattgt tattcatagg ctaacgagca gttgtaagcg ccctttttag
     8881 agcgtagtgt aagctggggt ttacgctacg ctctaaaaag ggtgcttact ctgaaacggt
     8941 gcggggaatt aatacaaggg cattgagatc tgcccgggat gataccgcca gaacccattg
     9001 cccagactat gggaaacaca ccaagttttc cctggaatgg aaaacaggcc tggttagtgt
     9061 gtgtcgctgg gctggtatgg gaaatagccc aggttcatta ttatgggaaa caaacctggt
     9121 tagtttttcg aatcaacaat ggtgaagact aacctgccat cttcattctg tgtcattgcg
     9181 gctttaagtg gtgtatttgc gtttatcttt ttcagtgccg gattgataaa cgttctcttc
     9241 atttcagcga agtttgatct ttgcgattca ggaagcatga atcgctctga cagccattcc
     9301 tgagtagtaa cccatactcc gctggaacgg tactggcaca ggctttcata cagtcgaatg
     9361 gccctaacac tgtttatttt tccgcagtca tagagtgaat acgtggtgaa ctggctggtc
     9421 agtcccatca gatagggcat gactttgtaa ttaaattcaa tttgccattt tccgcgccct
     9481 ttttttagcc cagcctcagc cagccagggc cgtttgatct cgtcaaactc tccgtctttg
     9541 ggataaaaca caacactact ttcagccagt gcagtgacgc cctttttaac atcagatgac
     9601 gctgtatcaa cagacacttt gaaatacttt tgataatcag caacaagaac tgtgaatact
     9661 ggcgataccg aatcaggatc atctttcagt ggatagcact gaataaggca taaccatagc
     9721 accctcttgg cttgcaacgg caagtagtaa gctgcttctg tcagttcatt ggattgacta
     9781 atgttgactg attttttcgc ggtacttatt gtcattgtca ttatcgtatg ggttgttttt
     9841 aactcgccca tattgtcatg caaaagccca tccaacaagg gaaattaacg tgttatccac
     9901 agtcataaaa cacgaattta aaccaggggc gtttcccata gcaatacgag gtgtttccca
     9961 taccaactcg tggcatttcc cataaaaatt cgggctgtta cccatattaa tccgtggtat
    10021 ttcccagcat aaccctgggt gattcccata aaggaggcct gaaaggcaga catgacgcgg
    10081 tctctgggca tcctaaaatg gacaaaaagg agaaaacttt taaaaaggaa taaaaatagc
    10141 ttaaaaaaca gtctgtgagt atgtggaaaa gttgatctga actgtcagag aaaactttgg
    10201 gtgtttccca caatatcctt taaatcaggt gcgtacgagc attatgtgac tatagcgccg
    10261 ttgcgcttcc tcgccagttc gctcgcgatg ctcggtctca aggctgcggc gagcactacc
    10321 tattcctttt ggtgataaat ataatgccac gtacattaat ttaatgtcgt ggttataaat
    10381 gttgtacgtt ttgttatgtt gtgtacaatg tgattaagct ttacacatat ttgacgcgca
    10441 aggaatccat ctttagggga aggggaaaac aatgagacgt gattacggta gtgttggcac
    10501 catagctctc agagcaagtg ccttacttca ggctatgagc agggatattg aggctcagag
    10561 aaaagaattt aatctgactc aatatcatca gacatatacc agaaatgctg tggcaaaact
    10621 gcctaagtta agccgtcgca tcgttgaact tgctgtaaaa gagatggagg acagcggtta
    10681 cgaattcaat aaaaagcagg ttggtaacgt agagcaatac gctctcacta ttcagaacgt
    10741 tatcgacatt tacgcccacc gtcaaatacc taagtaccgc gatattcaca aagaaccata
    10801 cgtgattttt gtcgtcaatc ttaaaggcgg tgtatcaaaa acagtcacga cagtcactct
    10861 cgcacatgca ttacgagtac atccggactt gcttcgtcat gatctccgca tcctggttat
    10921 tgaccttgat ccgcaggcat ccagtactat gtttcttgat cacactcata gcattggtac
    10981 tgttcttgaa acagcagccc aggcaatgct gaatgatctc gatgcagaaa cgttgagaga
    11041 agctgtaatt cgcccaacta ttatcccagg cgtcgatatc atccctgcgt cgatcgacga
    11101 cggctttgtt gccagccagt gggagtcatt agttacagaa catttaccag gtctgaaacc
    11161 ttctgaggtg ctcaaaaaaa caattattga ccgtatcgca ggtgattatg attttgtctt
    11221 tattgataca ggccctcacc ttgatccgtt cctcctgaat ggactggccg cgagcgattt
    11281 gctcttgacc cctactccac cggcacaggt tgattttcac tccacgttga aatacctgac
    11341 acgtttaccg gagatgctgg aacgccttga agaagaaggt gtggaaccca ggctcagcgc
    11401 cagtattggg tttatgtcaa aaatgaccgg caaacgtgac cacgagacgt cacatagtct
    11461 ggcgcgtgaa gtgtacgcaa gtaacattct tgatgcgtca ctgccgcgac ttgacggatt
    11521 cgaacggtgt ggtgagtcgt ttgacaccat tattagtgca aaccccgttt cctaccctgg
    11581 aagtgctgaa gcactgaaaa aagcccgaac tgaagctgaa cggtttacta aggctgtttt
    11641 tgatcgaatt gaatatatcc gtggagcatc aaaatgagga aagttttagc tcgtggccga
    11701 gttcttggca acagcaattc agaatttgcg cgaatgcttg aaagcgatgg cgatgtgaaa
    11761 acctttactc tcaaatctgg taaacaggcc aaatttgtga aaacggttgt gctaagcggt
    11821 gagatcgaat cgaagacatt tgttgattca tctgttaatg gtcgcgacca ggcaatgtta
    11881 tcccctgagt cagtcagtga catttcccgt accataaaac tgcagcagtt tttccccgct
    11941 attgggagag aggtcaacgg gcgaattgag atcctggacg gcacacgtcg ccgggcagcc
    12001 tgcatttata acaatgttaa attcgaaata cttgttacaa aggacgatat tgaactgtca
    12061 gatgcacgac aactggcaaa agacatacaa accgcacgtg agcatagctt gcgtgagctg
    12121 ggaaaacgac tcgaagttac gtatggaacc agtatgacga aggaagagat tgctcaggca
    12181 gaaaatctct ctcctgcgaa agtgactcgt gcgtttcagg ccgcagcagt gccagatgaa
    12241 atggttgcag ttttccctgt gataaatgat atttcgttgt cggattatca ttttttactg
    12301 aaactagctg aagaagcaaa caacaagcaa agatcagtaa cagaactgat ggaaaaagtt
    12361 cagcatcggt tgaagaccat gccagattat ccggcaattg ataaaagcaa aattctggct
    12421 gttatcagag ctgagagcaa atcgctgata gttcgcccga cccgcacggt tcaaactgag
    12481 aagctgagag aattttcaga tcgcaaccaa tttgcaagaa agaaaattga ttcaaagaaa
    12541 cgacttgttg tttatgagtt ttcccgcatt tccgctgagg cacagtcgga gatagatgag
    12601 gcaataaaac gtattctgga aagacttcct gagtcaagtg agtaagcgac aaggatgccg
    12661 gagagcatcc ttttcttatg tttttcacca tgcctatttc atggttactt ctttgattat
    12721 aaaagaaaaa taataattct ttcacactga aatcaccact ttttctcaac ttctacgtga
    12781 ctcataattt cgctactggt agttcagacc agcgagttgt ataggctggt gaaagcatct
    12841 cccgtttcat ttgccattca gatgcaatcc cccggcccgc aaaccacact tttccgagtc
    12901 ctgaatgatt aataccgtcc acaactttca ttagctgatt gctgttgctt cggagcagag
    12961 tatcatcgaa caggttcaac agcgaaaccc cgtcaggagc gaaatcgttc agcatacagc
    13021 ctgcctttgc gtaacggtga ccatccaccc agatcctgtc cagagctctg acggcagcgg
    13081 caatgatgtc ctgtgtatcc tgcgtgggga tgagcagttt ttcactggcc atattaccgt
    13141 agtagggctc atttacggcg aacggcgatg ttttcacaaa cacggcaata tgcctgcaga
    13201 actgacgctc gccgcgcagc ttttcagctg cacgctcagc gtactgacaa actgcctgtc
    13261 gcatggcttc ataagttgtg acacgttccc caaagctcct gctgcagact atctgctgtt
    13321 tggggggcgg tgcttcctcc agtgaaatac agctttctcc ggtgagttcc cggaccgttc
    13381 tctccagaac cacattgaag tttttcctga taaatacagg ggtcgcgcgc gccagctgca
    13441 gtgcagttgt tatccccatt gtgttcagct tctttgagat cctgcggcct accccccaga
    13501 tttcctccac cggctgcagt gacagcagtt tttccgtacg gcggatattg cctagcgtta
    13561 acgccagcac gccaccaaac tatggccact ctttggaagc ccactgtgct gactttgcta
    13621 gcgttttggt tggccccatt ccgacaccga tagtcaggcc agtcccgttc ctgacgtgtt
    13681 cacgcagctg ccggccgaaa tcctcgaaat cgatgcagct gccaataccc cgcacgtcga
    13741 gaaacatctc atcaatactg tactgctcca cgcccggcac cagctcctcg agatgagaca
    13801 taaccctgtt cgacatcgat gcgtatagcg cgtaattact ggagaaggcg atgaccggct
    13861 ccggaaattt caccgacttc agctgaaacc acggtactcc catttttatg cccagccgct
    13921 tcgcttctat gttatttgga attgactata tttcatacaa cacattctgt tatcgaaaag
    13981 ggggatgctg tgcacaagaa actgctggat gcgctgcctt tcctgtcggc cgatccttca
    14041 gcttgccggt gggtgtgcct gcaggaggac ctgctgtgtg ctccaaacac cattgatgcc
    14101 tatgcccgtg gcgtcaatga ctggctcgct ttctgtcact ctgttgccct gacggctgct
    14161 gtcgctggac gtgatacggt cgcactctat gtccgcagcc tgcataccgg tcgccagctg
    14221 gccgctgcca ctatccgcca ccggctgacg gtggtacgac tgtacaacga ttggctttgt
    14281 gaggagggga tacgggagag aaatcctgtc agacgaggtg tctggaaaaa cggagggaaa
    14341 ggaaaagctg gtatagtgcc gttgcagcgg cggctaccgt ggatcccgga tgatgcggag
    14401 tggttgcgct ttcttgaggt agccggacaa gctgatatcc gtactcgttt tatgctggca
    14461 ctggcctacg actgcggatt acgcagagaa gaactttgca cagtagctac cggagatatt
    14521 gacccttctc aacgcttgct cactgtgcgc gctgagcata ccaaaaaccg ctttggtcgg
    14581 gttgtgccct attctcctgt gacgggggaa ctctacacag catggctcac tgaacgtcgt
    14641 atgctaagct catctcgcgg tccgttgttt ctctcacgct ctccgcgtaa tcgggcagaa
    14701 cctattagca actggacttg gtcaaaagta gttcgcggtt tagctattaa ggctgatctt
    14761 ccactcatca gcacccacac attccgacat ctttgtctga cagaactggc acgcgtgggg
    14821 tgggacatcc acgaaattgc tgcattcgct ggccaccgtc gtatccagtc aactctgctc
    14881 tatattcatc tcagcgcacg tgatctgagt agtcgtttca attacacggt cgcatccttg
    14941 catacttgcc gccttactgt actacaacag caggatcctt ctccatgatg caatctgaac
    15001 atacagcgcc ttgtccgaca acatcactca gtcttcctgc cctactttgg gatacccgac
    15061 cggagatctc tgaaagcgag cttgcggcac tggatacatt ggtcgaccac ttccagcaag
    15121 gaggtaaaaa ctggtcacct gatatacaaa aacggctttc ccgccttctt ttaccactac
    15181 gcgacacgct aacaaaaatg catgcggcca aggcccccta caattcatct attcacgata
    15241 tagtcctgga gatgcagcgc atccggaaaa catactgggc ctggacacaa gaagagtggt
    15301 tagaagttat ttgcaacagc gaaggggaat ttcgtcgacg gtttggagcc agaggcaact
    15361 gtcggcaata tgttatagcg ttagcatggt tgctatgtgg gtttgagcgg ctggaacact
    15421 gtggcatatt ctatcaatat cggctctgtc ttaaggtttt tggccgacag agcaccgatt
    15481 ttgccgtcag ccagcttgac aatatgatgc aggttctggg atatgtgcct cgtgatagtc
    15541 ggaacaacgg catcagaaat gccatgtgta tggcaatgct tctgcaaagg gatgctcaac
    15601 tagaccatat caccgtgacc acgttgcaac agattgctgc aacgtgcccg gattacctcc
    15661 gggaagcctc agctactcta tcgcgtatac tggctgcatc agggactatt gaggaagggt
    15721 ttgattaccg aataacacaa cgtcgccgtc ctccccgcga atataatgca acagccgatg
    15781 taccaactaa atggctggta tggtgcaaac ggtggcgtgc tacctcagta cttcgccctt
    15841 catctatttt gtccggttgg tacgttttgc taaagtgtgg tcagctggtt agctgagtgc
    15901 tatccaacct gtctgtcacc atctgattgg acacgggaca ccgcgatcgc ctgggtgacc
    15961 gccgcttgta gaatgaaagt tggtgaatgg gcaaaccctg ggggcatgta tcgtgaccgt
    16021 cgtggcaaaa tgatgatgcc tgcagcgaga gctaggatgt tgggtcgtat tagaactttc
    16081 tttcatgact tgcaggaatg gggatggata cctgtacgat tcagcccaga aagagttttc
    16141 agggcaccgc gtagcttgac ttccctggtt ggaccagagc ctcgtattgt ggctgatgat
    16201 atgtggtgta aattacttca cgccggacag aacctgcagg agtcggattt accaaactgt
    16261 gttgcatatc cttattttta tccactggag atggtcagag cactcagcgt tctctggtta
    16321 tttggaggcc tcagacgtga tgaaattcta cgtatgcagt gtgggtgtat ccgctggcaa
    16381 cagccagaag aaaataatgt atcccgtatc tgtctgatag atgtacccgt tagcaagact
    16441 tacgcagcat tcacaaagcc agttgaccct attatcgggg aatatatcga acaatgggaa
    16501 cttgtctgta acccccaccc cctccaggaa gattctaaaa caggtgaaag cgtccgtttt
    16561 ttgttcatgc acagggggaa acgggtacac agtagttata ttaataattc gctaatccct
    16621 ttgctatgca gaaaggcagg tatacctgag caggatgcca gaggtaaaat caccagccat
    16681 cgggcaagag ccactatagc cagtcagctt tacaacgcca gggaaccact tgatattttt
    16741 gagctacaga aatggttggg gcattcttca cctgaatcta cccgacatta tgtcgaaatt
    16801 acaccgacga ggttggcagg atcactggat aaagctggtt actttgaacg taatcgccgc
    16861 atggtttcag ttctcattga tcaagacgcg atagctgaag ggctggtagc atccgggaag
    16921 ccatggcgtt attatgatct tggacacggt ttctgtactt atgacttttt tgagcaatgc
    16981 ccccatcgga tggcatgtgc aaaatgctca ttttatatgc caaaatcttc atctgaagca
    17041 caatacttgg aaggtcgcca gaatctactc aagctgatgc aggaaatccc tttgactgat
    17101 gacgaacaag ccgctgtaga aaacgatata caggcagtgg acaaactttt gaataagctc
    17161 actgggggcg cgcccccggg aaataagaga aagtaataaa atagctcagc actaatttca
    17221 ttttaattta aatacatggt aatgactcca acttattgat agtgttttat gttcagataa
    17281 tgcccgatga ctttgtcatg c
//
LOCUS       DAJOWB010000101        16857 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJOWB010000101 DAJOWB010000000
VERSION     DAJOWB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.101"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            137..811
                     /locus_tag="OHO85_003568"
     CDS             137..811
                     /locus_tag="OHO85_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672891.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARN
                     ASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKF
                     RRDNVKEIISNLFANKVDVDHPENKYKDFKDLEDKVEKRLQNRQTEYQNEINQLSAPG
                     VNFDDI"
     gene            complement(993..1748)
                     /gene="mlaA"
                     /locus_tag="OHO85_003569"
     CDS             complement(993..1748)
                     /gene="mlaA"
                     /locus_tag="OHO85_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCQ2672892.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            2042..2974
                     /locus_tag="OHO85_003570"
     CDS             2042..2974
                     /locus_tag="OHO85_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCQ2672893.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3050..3124
                     /locus_tag="OHO85_003571"
     tRNA            3050..3124
                     /locus_tag="OHO85_003571"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3083..3085,aa:Arg,seq:cct)
     gene            complement(3566..4813)
                     /locus_tag="OHO85_003572"
     CDS             complement(3566..4813)
                     /locus_tag="OHO85_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HCQ2672894.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(4885..5799)
                     /locus_tag="OHO85_003573"
     CDS             complement(4885..5799)
                     /locus_tag="OHO85_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HCQ2672895.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            6015..7448
                     /gene="cscA"
                     /locus_tag="OHO85_003574"
     CDS             6015..7448
                     /gene="cscA"
                     /locus_tag="OHO85_003574"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCQ2672896.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(7456..8514)
                     /locus_tag="OHO85_003575"
     CDS             complement(7456..8514)
                     /locus_tag="OHO85_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCQ2672897.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            8694..8912
                     /locus_tag="OHO85_003576"
                     /pseudo
     CDS             8694..8912
                     /locus_tag="OHO85_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            8930..10258
                     /gene="dsdA"
                     /locus_tag="OHO85_003577"
     CDS             8930..10258
                     /gene="dsdA"
                     /locus_tag="OHO85_003577"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCQ2672898.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(10366..11904)
                     /gene="emrY"
                     /locus_tag="OHO85_003578"
     CDS             complement(10366..11904)
                     /gene="emrY"
                     /locus_tag="OHO85_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCQ2672899.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGIG"
     gene            complement(11904..13067)
                     /gene="emrK"
                     /locus_tag="OHO85_003579"
     CDS             complement(11904..13067)
                     /gene="emrK"
                     /locus_tag="OHO85_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCQ2672900.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQF"
     gene            13483..14097
                     /gene="evgA"
                     /locus_tag="OHO85_003580"
     CDS             13483..14097
                     /gene="evgA"
                     /locus_tag="OHO85_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCQ2672901.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            14102..>16857
                     /gene="evgS"
                     /locus_tag="OHO85_003581"
     CDS             14102..>16857
                     /gene="evgS"
                     /locus_tag="OHO85_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCQ2672902.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLS"
BASE COUNT         4945 a         3877 c         3679 g         4356 t
ORIGIN      
        1 tacagaccat attactattg gtatcgacaa ccaagaacca atagaattac ctgcgaacta
       61 taaagacaaa gaactcgtcc gtactatcat taatgacaac atagttgaga agactcatga
      121 tatcaataac aaggaaatga tcttcagcgc attaaaagaa atatatgatg gagatcctgg
      181 ttttatcttc gataagatat cacacaaact cagacatacg gtaacggaat ttgatgagag
      241 cgggaatagc gaaccaacgg acttatttac ctggtacggt aaagataaaa aaggcgactc
      301 tctcgctatt gtaattaaaa ataaaaacgg aaatgattac ttatctctcg gttactacga
      361 tcaggacgac taccacattc aaagaggaat tcgtattaat ggtgatagtc tcacccaata
      421 ttgtagtgaa aaagccagga atgcttcagc gtggtttgaa agcagtaaag ctatcatggc
      481 agaatcattt gcaacaggtt ccgatcatca ggttgtaaac gagctcaacg gggaaagact
      541 gagagaacct aacgaagttt ttaaacgttt aggtcgagca ataagatata attttcaagt
      601 ggacgatgca aaatttagac gcgacaatgt aaaagaaata atttctaatt tattcgctaa
      661 caaagttgac gttgaccatc ctgaaaataa atacaaagac tttaaggatc tcgaagataa
      721 agttgaaaaa aggcttcaaa atcgccagac ggaatatcaa aatgaaatta atcaactatc
      781 tgcgccaggt gttaattttg atgatattta aaattatttt tccagttttt tgatttttca
      841 actcctgaat atcggaaata gtcagtgacg aacatcagta tttttacatc attacgtccg
      901 ggtcattgtc ggtttatctc cttttacttg tggtaattga gggataacaa caaaaaaggt
      961 gagtattgcg actcaccttt tttatttgtt tcttattcag aatcgatatc ttttaaatca
     1021 tcctgaatcg cttgcgcgtt cggattttcc tgcggtttga gttcgccgcc attagcgatg
     1081 aaatcatgac gctggaagta cgcttcgcgc accataatat aaggatcgga cgactgacgc
     1141 agcagaccat cggaatccaa caactgcgca cgagtttcta tcccttcaag cgtccattta
     1201 cccacagaca tcggccaggt cagccaggaa agaaccgggt aaagaccatc cgccatatca
     1261 ccaccgtcat cacgcagcgt gaagctaccg tagaacggta actggacgta aggcccataa
     1321 cccacgccat aatgaccaag cgtactaccg aagcggtgag gttcagtccg ttgcagtttc
     1381 gggttcgcca tccctgcaac atcaataaaa ccgcccatcc ccaaaatggt gttcaggaaa
     1441 aagcgggtaa agtggaccat cccctgataa gggtcgccct gcaagaagta gttaaccatc
     1501 accgcaggtt cttcaaggtt gccagtaaag ttgctcaaac cgttacgcgc cggttgcgga
     1561 acataatcac gccaggcgac agcgaccggt cgaacaatat acgggtctaa tacattgaag
     1621 ttgaagttgt acatggtgcg gttgaaccct tctaacgggt cagaacgccc ttgctgatct
     1681 gtaccggaac tcgcacaccc caccagaagc gtagttccca gagcaagcgc cgacaggcga
     1741 agcttcataa atgtctccct gtttttttat ggcttatgca gtttgccatc catgacggaa
     1801 cgataccgta tccgcctgtt taggtgtggg cgattgtaac agcacgtcaa ctgatgtcca
     1861 gacgccctga tttgctgatt tgatcatagc ctggtaatcg ccgccctgta ggctacttga
     1921 ttctatagaa acagaaaaag gcaaacgcag ccttttctac attttcagag taactcccgc
     1981 cagttgcgag caaaaaagcc gctacgcttt agctatacgt gctaattcaa gagaagagac
     2041 catggacaac gacaaaattg atcaacacag cgacgaaatt gaagttgaga gcgaagaaaa
     2101 agagcgcggc aaaaaaatag aaatagatga agaccgactc ccctcccggg cgatggcaat
     2161 tcatgagcat atccgccagg atggtgaaaa agagctggaa cgcgacgcaa tggcgctact
     2221 gtggtcagcc attgcggcgg gtctgtcgat gggcgcttcg ctactggcaa aagggatatt
     2281 tcatgtcgaa ctggaaggag tgccaggcag cttcttactg gagaatctcg gttatacctt
     2341 tggttttatt atcgtcatta tggcccgcca gcaattattt accgaaaaca ccgtgactgc
     2401 ggtactaccc gtcatgcaaa aaccgacaat gagcaacgtc ggcttactta tgcgattatg
     2461 gggcgtcgtg ctgctgggta atattctcgg gacaggtatt gctgcatggg catttgaata
     2521 tatgcctatc ttcaatgaag aaactcgcga tgcatttgtc aaaatcggca tggatgtgat
     2581 gaagaacacc cccagcgaga tgtttgccaa cgcgatcatt tccggctggc tgatcgccac
     2641 tatggtttgg atgtttcctg ctgcgggtgc ggcaaagatt gtggtgatta tattgatgac
     2701 ctggcttatt gcactgggtg acaccaccca catcgttgtc ggttctgttg aaatcctcta
     2761 tctggtgttt aacggcacgc tgcactggag cgatttcatc tggcccttcg cactacctac
     2821 tttagcgggg aacatctgcg gcggcacctt tatcttcgcg ttaatgagtc atgcacagat
     2881 tcgtaacgac atgagcaaca agcgtaaagc agaagcacgc caaaaagcag aacgtgcgga
     2941 aaacattaag aaaaatgata aaaacccagc ataaatggcg agggtttaag caatcgagcg
     3001 gcagcgtact taccccgcac tccattagcg ggtatactca tgccgcattg tcctcttagt
     3061 taaatggata taacgagccc ctcctaaggg ctaattgcag gttcgattcc tgcaggggac
     3121 accagatacc cttcaaacga aatctacctt caccccgtaa aagatgggtt tggcagcaca
     3181 cttgccttat atctactcat ttttactgca acaggttgaa atctcagcac tgtcagaaag
     3241 cgctgatgac taaacagccc tgggccgggc gatgtaacca tcacacagaa tcctgatagc
     3301 gaaatatggc gtgactcgat acttcactcc gcaatgcatt ccttgatgaa ttcgcaggac
     3361 cgtgatacac gggacaggtc actgaatgac gacaatgtcc tggaaatcag cgaaccgcgc
     3421 atctgaagta catttgagcg actgtaccag aacatgaatg aggcgtttgg attaggcgat
     3481 tattagcagg gctaagcatt ttactattat tattttccgg ttgagggata tagagctatc
     3541 gacaacaacc ggaaaaagtt tacgtctata ttgctgaagg tacaggcgtt tccataacta
     3601 tttgctcgcg ttttttactc aagaagaaaa tgccaaatag caacatcagg cagacaatac
     3661 ccgaaattgc gaagaaaact gtctggtagc ctgcgtggtc aaagagtatc ccagtcggcg
     3721 ttgaaagcag cacaatccca agcgaactgg caatttgaaa accaatcaga aagatcgtcg
     3781 acgacaggcg cttatcaaaa tttgccacgc tgtatttgaa gacggatatg acacaaagtg
     3841 gaacctcaat agcatgtaac agcttcacta atgaaataat ccaggggtta acgaacagcg
     3901 cgcaggaaag gatacgcaac gccataatca caacaccgat aagtaatgca ttttttggcc
     3961 ctacccgatt cacaaagaaa ggaataatcg ccatgcatag cgcttcgagt accacctgga
     4021 atgagttgag ataaccatac aggcgcgttc ctacatcgtg tgattcgaat aaacctgcat
     4081 aaaagacagg aaaaagttgt tgatcaaaaa tgttatagaa agaccacgtc cccacaataa
     4141 atatgacgaa aacccagaag tttcgatcct tgaaaactgc gataaaatcc tcttttttta
     4201 cccctcccgc atccgccgct acgcactggt gatccttatc tttaaaacac atgttgatca
     4261 tcataaatac agcgccaaat agcgagacca accagaagtt gatatgggga ctgatactaa
     4321 aaaatatgcc ggcaaagaac gcgccaatag catagccaaa agatccccag gcgcgcgctg
     4381 ttccatattc gaaatgaaaa tttcgcgcca ttttttcggt gaagctgtca agcaaaccgc
     4441 atcccgccag ataccccagg ccaaaaaaga gcgcccccag aattagacct acagaaaaat
     4501 tgctttgcag taacggttca taaacgtaaa tcataaacgg tccggtcaag accaggatga
     4561 aactcataca ccagatgagc ggtttcttca gaccgagttt atcctgaacg atgccgtaga
     4621 acatcataaa tagaatgctg gtaaactggt tgaccgaata aagtgtacct aattccgtcc
     4681 ctgtcaaccc tagatgtcct ttcagccaaa tagcgtataa cgaccaccac agcgaccagg
     4741 aaataaaaaa gagaaatgag taactggatg caaaacgata gtacgcattt ctgaatggaa
     4801 tattcagtgc cataattacc tacctgtcgt taaaaaattc acgtcctatt tagagataag
     4861 agcgacttcg ccgtttactt ctcactattc cagttcttgt cgacatggca gcgctgtcat
     4921 tgcccctttc gccgttactg caagcgctcc gcaacgttga gcgagatcga taattcgtcg
     4981 catttctctc tcatctgtag ataatcccgt agaggacaga cctgtgagta acccggcaac
     5041 gaacgcatct cccgcccccg tgctatcgac acaattcaca gacattccag caaaatggtg
     5101 aacttgtcct cgataacaga ccaccacccc ttctgcacct ttagtcacca acagcatggc
     5161 gatctcatac tcttttgcca gggcgcatat atcctgatcg ttctgtgttt ttccactgat
     5221 aagtcgccat tcttcttccg agagcttgac gacatccgcc agttgtagcg cctgccgcaa
     5281 acacaagcgg agcaaatgct cgtcttgcca tagatcttca cgaatattag gatcgaagct
     5341 gacaaaacct ccggcatgcc ggatcgccgt catcgcagta aatgcgctgg tacgcgaagg
     5401 ctcggcagac aacgcaattg aacagagatg taaccattcg ccatgtcgcc agcagggcaa
     5461 gtctgtcgtc tctaaaaaaa gatcggcact ggggcggacc ataaacgtaa atgaacgttc
     5521 cccttgatcg ttcagatcga caagcaccgt ggatgtccgg tgccattcat cttgcttcag
     5581 atacgtgata tcgactccct cagttagcag cgttctttgc attaacgccc caaaaggatc
     5641 atcaccgacc cgacctataa atccacttat tccgcctaat ctggcgattc ccaccgcaac
     5701 gttagctggc gcgccgccag gacaaggcag taggcgcccg tctgattctg gcaagagatc
     5761 tacgaccgca tcccctaaaa cccatacttt ggctgacatt tttttccctt aaattcatct
     5821 gagttacgca tagtgataaa cctctttctc gcaaaatcgt catggattta ctaaaacatg
     5881 catattcgat cacaaaacgt catagttaac gttaacattt gtgatattca tcgcatttat
     5941 gaaagtaagg gactatattt ttataaaagt taacgttaac aattcaccaa atttgcttaa
     6001 ccaggatgat taaaatgacg caatctcgat tgcatgcggc gcaaaacgca ctagcaaaac
     6061 ttcatgagca ccggggtaac actttctatc cccattttca cctcgcgcca cctgccggat
     6121 ggatgaacga tccaaacggc ctgatctggt ttaacgatcg ttatcacgcg ttttatcaac
     6181 atcacccgat gagcgaacac tggggaccaa tgcactgggg acatgccacc agcgacgata
     6241 tgatccactg gcagcatgag cctattgcgc tagcgccagg agacgataat gacaaagacg
     6301 ggtgtttttc aggtagtgct gtcgatgaca atggtgtcct ctcacttatc tacaccggac
     6361 acgtctggct cgatggtgca ggtaatgacg atgcaattcg cgaagtacaa tgtctggcta
     6421 ccagtcggga tggtattcat ttcgagaaac agggtgtgat cctcactcca ccagaaggaa
     6481 tcatgcactt ccgcgatcct aaagtgtggc gtgaagccga cacatggtgg atggtagtcg
     6541 gggcgaaaga tccaggcaac acggggcaga tcctgcttta tcgcggcagt tcgttgcgtg
     6601 aatggacctt cgatcgcgta ctggcccacg ctgatgcggg tgaaagctat atgtgggaat
     6661 gtccggactt tttcagcctt ggcgatcagc attatctgat gttttccccg cagggaatga
     6721 atgccgaggg atacagttac cgaaatcgct ttcaaagtgg cgtaataccc ggaatgtggt
     6781 cgccaggacg actttttgca caatccgggc attttactga acttgataac gggcatgact
     6841 tttatgcacc acaaagcttt ttagcgaagg atggtcggcg tattgttatc ggctggatgg
     6901 atatgtggga atcgccaatg ccctcaaaac gtgaaggatg ggcaggctgc atgacgctgg
     6961 cgcgcgagct atcagagagc aatggcaaac ttctacaacg cccggtacac gaagctgagt
     7021 cgttacgcca gcagcatcaa tctgtctctc cccgcacaat cagcaataaa tatgttttgc
     7081 aggaaaacgc gcaagcagtt gagattcagt tgcagtgggc gctgaagaac agtgatgccg
     7141 aacattacgg attacagctc ggcactggaa tgcggctgta tattgataac caatctgagc
     7201 gacttgtttt gtggcggtat tacccacacg agaatttaga cggctaccgt agtattcccc
     7261 tcccgcagcg tgacacgctc gccctaagga tatttatcga tacatcatcc gtggaagtat
     7321 ttattaacga cggggaagcg gtgatgagta gtcgaatcta tccgcagcca gaagaacggg
     7381 aactgtcgct ttatgcctcc cacggagtgg ctgtgctgca acatggagca ctctggctac
     7441 tgggttaaca taatatcagg tggaacaacg gatcaacagc gggcaaggga tccgcgtcac
     7501 tcttccccct tcacgacctt caataatatg caatgcagct tcccgcccga taatgtcatg
     7561 tggaagctga attgtggtca gcggcggtaa aaacagatgc ccgacgccaa ccagattatc
     7621 aaagcccatt acggcgacat cctgcgggat tcgtaccccc ttcgccagaa gaacctgata
     7681 agccacaaag gctgcgcgat cgttaccaca tatcagaaca tcaaaatctg gtttgcccga
     7741 tttgaagtgg tcattgagta aacttgcgag atcggtgtag tgatcatcac ctgttgccat
     7801 gtgaaattgt ttcacctcag ccagatctcg tccagcatca cgccaggcct gctcaaatcc
     7861 ctgccgacga taccctgttg ccaacgcact ttccggtagc cagaagcata acggttgacg
     7921 atagcccgcc gcgagcaaat gctgtgttga ttcatattgt gcagtgtaat catcagggat
     7981 ataactgggt aacgctgggt catccgccac acagttcgcc aatacaatat tttcaccata
     8041 cagagactca ggcagcgtga tatgtcgcag ccccattgta gtatagataa tgccatccgg
     8101 acggtgggca agcagctgac gtgccgcgcg ggcagcgtca tcttcagaaa aaatattgat
     8161 taaaaaacta ttccagccga actcgctggc ggtttgctca atggcaagca gaatatcaac
     8221 agagaaagga gtggtagccg tgtcctgcgc cagcacggcg agagtcgacg gcttacgtcc
     8281 ttgagcgcgc atcttacggg cggaaagatc aggaacataa ttcagggtct ggattgcctg
     8341 caatacgcgg tcacgcgttg caggacgcac agattctgca ttatgcatca cccgggagac
     8401 tgtcatcatc gacactcccg ccaggcgtgc gacatccttt aatgaagcca tacccaagcc
     8461 gtttgccgta aaacgggcac tgtagcagaa acagacgtca ctggcgagat ccaacgatat
     8521 tctcaacgcc ctatcacccg acacggcaat acaataaaaa ataacaataa ttgccggata
     8581 attgtactca attccgcctc tgttctcgcc aacgagtaac catataggca agtaagtaaa
     8641 acatgccaac tggcatcgcc aacctggaga agaaaatata aatatggatc attgtgctgc
     8701 cgctgtatcc cgacatcagc ccggaaatta ttgcgattgc tatcggttca ggtgcaattg
     8761 gctgcacgat cgttacggac tcgcttttct ggctagtgaa gcaatattgc ggcgctacgc
     8821 tcaatgaaac atttaaatac tatacgacag cgacatttat cgcttcagtc gtcgctctgg
     8881 cgggcacatt cctgctgtca tttatcatct aagcgcaaag agacgtacta tggaaaacgc
     8941 taaaatgaac tcgctcatcg cccagtatcc gttggtaaag gatctggttg ctcttaaaga
     9001 aaccacctgg tttaatcctg gcacgacctc attggctgaa ggtttacctt atgttggcct
     9061 gaccgaacag gatgttcagg acgcccatgc gcgcttatcc cgttttgcac cctatctggc
     9121 aaaagcattt cctgaaactg ctgccactgg ggggattatt gaatcagaac tggttgccat
     9181 tccagctatg caaaaacggc tggaaaaaga atatcagcaa ccgatcagcg ggcaactgtt
     9241 actgaaaaaa gatagccatt tgcccatttc cggctccata aaagcacgcg gcgggattta
     9301 tgaagtcctg gcacacgcag aaaaactggc tctggaagcg gggttgctga cgcttgatga
     9361 tgactacagc aaactgcttt ctccggagtt taaacagttc tttagccaat acagcattgc
     9421 tgtgggctca accggaaatc tggggttatc aatcggcatt atgagcgccc gcattggctt
     9481 taaggtgaca gttcatatgt ctgctgatgc ccgggcatgg aaaaaagcga aactgcgcag
     9541 ccatggcgtt acggtcgtgg aatatgagca agattatggt gttgccgtcg aggaaggacg
     9601 taaagcagcg cagtctgacc cgaactgttt ctttattgat gacgaaaatt cccgcacgtt
     9661 gttccttggg tattccgtcg ctggccagcg tcttaaagcg caatttgccc agcaaggccg
     9721 tatcgtcgat gctgataacc ctctgtttgt ctatctgccg tgtggtgttg gcggtggtcc
     9781 tggtggcgtc gcattcgggc ttaaactggc gtttggcgat catgttcact gcttttttgc
     9841 cgaaccaacg cactcccctt gtatgttgtt aggcgtccat acaggattac acgatcagat
     9901 ttctgttcag gatattggta tcgacaacct taccgcagcg gatggccttg cagttggtcg
     9961 cgcatcaggc tttgtcgggc gggcaatgga gcgtctgctg gatggcttct atacccttag
    10021 cgatcaaacc atgtatgaca tgcttggctg gctggcgcag gaagaaggta ttcgtcttga
    10081 accttcggca ctggcgggta tggccggacc tcagcgcgtg tgtgcatcag taagttacca
    10141 acagatgcac ggtttcagcg cagaacaact gcgtaatacc actcatctgg tgtgggcgac
    10201 gggaggtgga atggtgccgg aagaagagat gaatcaatat ctggcaaaag gccgttaata
    10261 acgtttcaac gcagcatcgc aatcctttcc ctgggtgagc gatgctgccg atggcgcaga
    10321 cttaagatcc ccggtcttac ccgctataac cccctccttt tatcatcacc caatgccttt
    10381 cgctgtaaac ggcggtttcg caaaccaaac caaaaccgta agtaagataa aagcaatagc
    10441 cgccatacgg aaaatctcat ttgcagaaat agaaagtgac tgctgggtta tttcattatt
    10501 aatttcatta agaactcctg aaagcgaacc atagtattta tccataattt gtgacgatga
    10561 attaaacacg gggttaaatt gatcgatggt tgctgtcaac tgactatggt gtaacgattc
    10621 gcgtcgtccc cacagcgtca ttgtcaacga cgtaccaact gatcctgaca aggtacgaaa
    10681 aaaattactc atactcgagg cattggcaaa tttattatct ggcaagcctg aaaacgaaat
    10741 cgttgttaag ggtaaaaaga aacaggcaac ggcgaatccc tgaaaaaact gcggcaaaat
    10801 gatgcctgta aaatcaatcg ttggcataaa tgtcacagaa cgccagtaat agcaaaccgc
    10861 atacatcaaa aaactaaatg tcactaacaa ccgcatgtct attttgttgc cataacgtcc
    10921 tatcaaaggt gaaattaata gtggcatgat gccgatgggc gcataagcaa gtccggccca
    10981 tatcgcatta taccccatcg tttcctggag taactgcggc ataaggacga tcgctccaga
    11041 gtaaaataaa tacgcgcatg tgatactcac aataccaatg gtgaagttac gggacttaaa
    11101 caaactgaga tcaagaatcg ggttctctga ggtcgactcc caaatgacta aagagatcag
    11161 agaaataact gatactactg ttaatattat tattgtactc gagttgaacc aatccagatc
    11221 gcgcccttta tcaagcataa tttgcaagcc accaacaccg agcactaaca gggtcagtcc
    11281 tggtagattc attttgaccg gtgaagtctc agtttctctt cctttaagta aggttaagca
    11341 taatgtcagg acgataatcc ccatagggac attgattaaa aatatccaac cccagctaaa
    11401 gttatcacaa atataaccgc ccaatatcgg cccacatatc ggagcgataa tcacggtcat
    11461 tgaccataat gccagagcaa atgttctttt ttctggcgga taattcctta ataataaact
    11521 ctgtgacagt ggaattaacg gccccgccat taacccctga acgactctaa aaaatatcag
    11581 cacatcaaga ttggtcgata ggctacacat taatgaagac agcgaaaaaa aggttactga
    11641 aagtaaaaat aatcttaatt cgcctattct ttgtgccaac ctgccagtaa cagggatcgc
    11701 aatggcattt gctacaccaa acgaggtgat aacccaggtg ccttcgtctg ttgatgctcc
    11761 cagaaagcca gatattgtcg gtattgcgac gttagaaata gtggaatcca acatttgcat
    11821 aaatgtcgct aatgacaatg caatagtgac gcaccataac gtcccaccgg ttaatggtgc
    11881 cggagttgat ttagtgattg ccattaaaat tgtccattat gcgaaataat gttgctaatt
    11941 tctttttcta tcggactggt atcgataact aaagccttac tggtataagc cggcatggag
    12001 gtcacggttg aagccagctc aggcatctcg gcaatgtctt cgttcttcgt atcaatagtt
    12061 gctgtcatcg ataaaccaat acgcaaggga tgttccatga gttcttttgg atcaagagaa
    12121 acttcaaccg gtacacgctg aacgattttg atccagttcc ctgtcgcatt ttgtgcaggt
    12181 aataaggaga acgcattgcc ggttcccata ttgatccctg tcacccgacc atgaaacaca
    12241 acattttcac cataaagatc gctgataata ttgaccgatt gaccaatccg tacatccgtg
    12301 agttgtgttt ctttaaagtt ggcattaacc cacatttgac gtgccggtac gacagccatt
    12361 aacgattgtc cggggctcac tgtttcgccg acctgaacac ttcgctgggc aatatagccg
    12421 gtaaccggac tcttaatatc cgtacgttta agcgccaacc aggcttcttt agttgcatcc
    12481 gccgcttcaa cgacttgtgg ctgacggttt aatggtgtgt tcattactaa agctttattc
    12541 gctttataag cctggatagc ggcattcaat gccgctttgc tacttattaa cgtatctttg
    12601 gtatgctcca gcgtttcttt tgaaataacc ccctgcttcg ctaacggcac tcgacggtta
    12661 taatcttcta aagattgttg atactgaata cgtgctgaag cgacttcggc actgtattgt
    12721 ttatcctgta agtatagttt attcgtttgc cgaacaatat ttgccagatt atttttagct
    12781 ttattgagtg cgatagtggc atcagtttta tccagtgaaa ctaaaatgtc accttgtcga
    12841 acgtagttcg tatctttatg attaacgaca gtgacactac ctgagacttg tgcagaaatt
    12901 ggatctgcat tccccgtgac ataggcgtca tctgtactaa tcatgtcttc taattccatt
    12961 gaccaatagg cataggcacc tgaaaacgca ataaataaaa ctaccgccaa taaagaaaag
    13021 tattttcttc tgttagaatg ttttttattt gaattaatct gttccactat tatctctcat
    13081 ttctcataga tgaaatttat gaattgaatg aaaagagcat tcattcttca tatcaaacac
    13141 agtgcatgtg ttgcacatta tcccaggaac gttggaggcc taatttaaat gaggtgcaaa
    13201 aaaaacaaca gaggactaaa ccgtggcttt tgcaatacaa ttcttacgcc tgtaggatta
    13261 gtaagaagac ttatagtgct aacttgaaac tataaatcac cggtacaatc cctgatttta
    13321 ttgttgacat ttcatttatg ccgactattt atatggtata cttgtcgaat tatcttaaag
    13381 gaagctcaga ttttcttatt tttattgaga aaatgagatg acgccttatg tctgtattac
    13441 tacagggaga agggagatgc ttcattgcaa agggaataat ctatgaacgc aataattatt
    13501 gatgaccatc ctcttgctat cgcagcaatt cgtaatttat tgatcaaaaa cgatattgaa
    13561 atcttagcgg agttgactga agggggaagc gccgttcagc gggtggaaac acttaagcct
    13621 gatatcgtca tcattgatgt cgatatcccc ggagttaacg gtatccaggt gttagaaacg
    13681 ctgaggaagc gccaatatag cggaattatt attattgtct ccgctaaaaa tgaccatttt
    13741 tacgggaaac attgtgctga tgctggcgct aatggattcg tgagtaaaaa agaaggcatg
    13801 aacaatatca ttgcggctat tgaagctgca aaaaatggct actgctattt ccccttctct
    13861 ctcaaccggt ttgttggaag tttaacgtcc gaccagcaaa aactcgactc cttatcgaaa
    13921 caagaaatta gtgtcatgcg gtatattctt gatggcaagg ataataatga cattgctgaa
    13981 aaaatgttca tcagcaacaa aactgtcagc acttataaaa gtcgcctgat ggaaaaatta
    14041 gaatgtaaat cactgatgga tctttacaca ttcgcgcaac gtaacaaaat cggctaatca
    14101 catgaagttt ttaccctatt tttttcttct ctgttgtggt ctttggtcga ccataagttt
    14161 cgcagacgaa gattacatcg aatatcgtgg catcagtagt aacaaccgtg tcacacttga
    14221 tccagtacgt ctgagcaata aggaattacg ttggttagcg agcaaaaaaa atcttgtgat
    14281 tgcagtacac aagtcccaaa cggctacctt gttgcatacc gattcgcagc aacaggttcg
    14341 tggtattaat gctgattatt taaatctttt aaaaagagcg ttaaatatca aattaacact
    14401 ccgggaatac gcagatcatc aaaaagcaat ggacgcgctt gcagaaggtg aagtcgatat
    14461 agtgttatca catttagtta cttcgccgcc tcttaataat gacattgctg caaccaaacc
    14521 attgataatt acctttccgg cgctggtaac cacccttcac gactcaatgc gaccgcttac
    14581 ctcaccaaaa ccagtaaata ttgctcgggt agcaaattac cccccagacg aggtaattca
    14641 tcaatcattt ccaaaagcaa caattatctc ttttacaaat ttatatcagg cattagcatc
    14701 cgtctcagct gggcacaatg attactttat tggtagtaac atcattacca gcagtatgat
    14761 ttcccgctat ttcactcact ccttaaatgt agtgaaatat tataactcgc cgcgtcaata
    14821 taattttttc ttgaccagaa aagaatctgt cattcttaat gaagtactca atagatttgt
    14881 tgatgcttta acaaatgaag ttcgctatga agtatcacaa aattggcttg atacaggaaa
    14941 cctggccttt ctgaacaaac cattagaact cactgaacat gaaaaacagt ggattaagca
    15001 gcatcccaat ttaaaggtgc tggaaaatcc ttactcgccc ccctattcta tgacggatga
    15061 aaatggctcg gttcggggcg ttatggggga cattcttaat attattacct tgcaaacagg
    15121 tttaaatttt tctccgatca ccgtttcaca caatatccat gctggaacac agcttagccc
    15181 cggaggatgg gatataatac ctggcgctat ttatagtgaa gatcgagaaa ataatgtttt
    15241 atttgctgaa gccttcataa caacgcctta cgtttttgtc atgcaaaaag cgcctgacag
    15301 tgaacaaaca ttaaaaaaag gaatgaaagt tgccattcca tattattatg agctgcattc
    15361 gcaattaaaa gagatgtatc cggaggttga atggatacag gtcgataatg ccagcgctgc
    15421 atttcacaag gttaaggaag gtgaacttga tgctctggtc gcgacacagt taaattcgcg
    15481 ttacatgatc gatcattact atcctaatga actttatcat tttcttattc ctggcgttcc
    15541 gaatgcatcg ctttcgttcg cttttcctcg cggagaaccg gaacttaagg atattattaa
    15601 taaagcactg aatgcaattc ccccaagcga agttctgcgc ctgacggaaa aatggattaa
    15661 aatgcccaat gtgaccattg acacatggga cctatatagc gagcaatttt atattgttac
    15721 gacattatcc gttttattag ttggcagtag ccttttatgg ggattctacc tgttacgctc
    15781 agttcgtcgt cgtaaagtca ttcagggtga tttagaaaac caaatatcat tccgaaaagc
    15841 actctcggat tccttaccga atccaactta tgttgtaaac tggcaaggta atgtcattag
    15901 tcataatagt gcttttgaac attatttcac tgcggattac tacaaaaatg caatgttacc
    15961 attagaaaac agtgactcac cctttaaaga tgttttttct aatgcgcatg aagtcacagc
    16021 agaaacgaaa gaaaatcgaa caatatacac acaggtattt gaaattgata atggcatcga
    16081 gaaaagatgc attaatcact ggcatacatt atgcaatctt cctgcaagtg acaatgcagt
    16141 atatatttgt ggttggcaag atattactga aacgcgtgat ctaattaatg cactcgaggt
    16201 agaaaaaaat aaagcgataa aggctaccgt agcaaaaagt cagtttctgg caacgatgag
    16261 tcacgaaata agaacaccaa taagctctat tatgggcttc ctggaacttc tgtcgggttc
    16321 tggtcttagc aaggagcaac gggtggaggc gatttcactt gcctacgcca ccggacaatc
    16381 actcctcggc ttaattggtg aaatccttga tgtcgacaaa attgaatcgg gtaactatca
    16441 acttcaacca caatgggtcg atatccctac tttagtccag aacacttgtc actctttcgg
    16501 tgcgattgct gcaagcaaat ccatcgcatt aagttgcagc agtacatttc ctgatcatta
    16561 cctggttaag atcgatcctc aggcgtttaa gcaggtctta tcaaatttac tgagcaatgc
    16621 tctcaaattt accaccgagg gggcagtaaa aattacgacc tccctggttc acattgatga
    16681 caaccacgct gttatcaaaa tgacgattat ggattctgga agtggattat cgcaggaaga
    16741 acaacaacaa ctctttaaac gctacagcca aacaagtgca ggtcgccagc aaacaggatc
    16801 tggcttaggc ttaatgatct gcaaagaatt aattaaaaat atgcagggcg atttgtc
//
LOCUS       DAJOWB010000102        16665 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJOWB010000102 DAJOWB010000000
VERSION     DAJOWB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.102"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(19..171)
                     /locus_tag="OHO85_003582"
     CDS             complement(19..171)
                     /locus_tag="OHO85_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCQ2672903.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(436..1170)
                     /gene="cysH"
                     /locus_tag="OHO85_003583"
     CDS             complement(436..1170)
                     /gene="cysH"
                     /locus_tag="OHO85_003583"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCQ2672904.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMLEEETRFFGLKRECGLHEG"
     gene            complement(1244..2956)
                     /gene="cysI"
                     /locus_tag="OHO85_003584"
     CDS             complement(1244..2956)
                     /gene="cysI"
                     /locus_tag="OHO85_003584"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCQ2672905.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2956..4755)
                     /gene="cysJ"
                     /locus_tag="OHO85_003585"
     CDS             complement(2956..4755)
                     /gene="cysJ"
                     /locus_tag="OHO85_003585"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCQ2672906.1"
                     /translation="MTTQVPPSALLPLNPEQLVRLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5074..5436
                     /gene="queD"
                     /locus_tag="OHO85_003586"
     CDS             5074..5436
                     /gene="queD"
                     /locus_tag="OHO85_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCQ2672907.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5514..6785
                     /locus_tag="OHO85_003587"
     CDS             5514..6785
                     /locus_tag="OHO85_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCQ2672908.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERCITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            6776..7036
                     /locus_tag="OHO85_003588"
     CDS             6776..7036
                     /locus_tag="OHO85_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCQ2672909.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTAERLISACPAGLFSLTPE
                     GDLRIDYRSCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7053..7628
                     /locus_tag="OHO85_003589"
     CDS             7053..7628
                     /locus_tag="OHO85_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCQ2672910.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIGDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7776..8636)
                     /locus_tag="OHO85_003590"
     CDS             complement(7776..8636)
                     /locus_tag="OHO85_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCQ2672911.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLIIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPKVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8633..9412)
                     /locus_tag="OHO85_003591"
     CDS             complement(8633..9412)
                     /locus_tag="OHO85_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCQ2672912.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWRDYLRQRMQP"
     gene            complement(9390..10799)
                     /locus_tag="OHO85_003592"
     CDS             complement(9390..10799)
                     /locus_tag="OHO85_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2672913.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSRVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10821..12275)
                     /locus_tag="OHO85_003593"
     CDS             complement(10821..12275)
                     /locus_tag="OHO85_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCQ2672914.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12345..13130)
                     /locus_tag="OHO85_003594"
     CDS             complement(12345..13130)
                     /locus_tag="OHO85_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCQ2672915.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAASCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13449..14726
                     /locus_tag="OHO85_003595"
     CDS             13449..14726
                     /locus_tag="OHO85_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2672916.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSTSLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYTS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14753..16231
                     /locus_tag="OHO85_003596"
     CDS             14753..16231
                     /locus_tag="OHO85_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCQ2672917.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNYFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         3991 a         4371 c         4179 g         4124 t
ORIGIN      
        1 tttacccccg cgtggtggct acttcttcga ttcgtaagcg agaactgcct taaactccat
       61 accacgttct ctgatactca actcacacaa cgagtcacct accatcagcg tgaatcccag
      121 tactgtacaa cacagtacga tgattgccac aagggcatat tttgtcagca tgtcttgcct
      181 ccctgcaaag aagagactaa cattcgaatt gtttgggttc gaacgtaagc ctcaggttga
      241 tagaaatatc gcctggggct tttgtccatc tggaacctcg cgaatgctta acgccagaca
      301 gcctcaagca cccgacgcca ttctatacct gataattctt ccagcggcgt tcacgtttaa
      361 aatcaggaat actgctcgca aaataaaaac gcccccatca tttctgacag aggcgtttaa
      421 tttatccggc aatatttacc cttcgtgcaa tccacattcc ctttttaagc caaagaaacg
      481 tgtttcttct tccaacatgc caggttccca tttacgggtt gtatgggtat caccgaccga
      541 taaatatcct tcatcccata atgggtgata tttcaggcca tgtttttgca ggtactgata
      601 aatagttcgg ttatcccagt cgataatcgg cagcacttta aatacgccac gctgaattgc
      661 cagcaccggt aaattggcgc ggctgccgga ttgttcgcgg cgcaggccag caaaccacgt
      721 ttgcgcattc agttctttca gcgcccggtt catcggttcg actttgttga tgtcattgta
      781 cttttcaatg ccttcaacgc cctgttccca cagtttgccg tagcgtgctt cctgccaggc
      841 ggcgctttcg gtagcgcggt acactttcag gttgagcttg agtttgtccg ttaactcgtc
      901 aataaagcgg taggtttccg ggaacagata gccggtgtcg gtgaggatca ccggaatatc
      961 cgggtgaatc tgattcacca gatgcaggct caccgccgcc tgaatgccaa agctggaaga
     1021 aagtacatat tcaccgggca gattatccag cgcccaggca acgcggcctt cagcgtccag
     1081 tttttccagt tcggcgttag tttctgccag cgccagaatg cgatctacct tcggcagttc
     1141 gttcagggcg tttagatcga gtttggacat agattcctca ctgtttgcct tgcctgatgc
     1201 gacgctcacg cgtcttatca ggcctacaag accgggctga tggttaatcc cacaaatcgc
     1261 gcgccggatc gagcaccggg cgaatgatgc ccgcacgcac cgtaaagtcg ccgaagcctt
     1321 cacccgcttc gcgctctttc gcccagcgcc ctatcagttc atcaagcgac gccaggattt
     1381 ccggctcggt gatgttttct ttatacatcc gtgggatacg tgtcccaatg cggttgccgc
     1441 caagatgcag attgtagcga cccggtgctt tacccaccag gcccacttcc gccagcatcg
     1501 cgcgaccaca accgttcggg cagcctgtta cacgcatcac gatatgctca tcgctgacac
     1561 catgtttcgc cattaaatta tcgatgttgt cgataaaaga cggcaggaaa cgctccgctt
     1621 ccgccatcgc cagcgggcaa gtcgggaatg acacgcaggc catcgagttt tcacgctgcg
     1681 gcgtgacggc attcattaaa ccgctctctt tggcgatctt ctcgatcttc gctttctcgc
     1741 tttccggtac accggcgatg atcagattct ggttcgccgt aatgcggaaa tcgcctttgt
     1801 ggatcttcgc gatctccagc aggccggttt tcagcggacg ccccggataa tcaaggatgc
     1861 gaccattttc gataaacagc gtcaggtgcc agttatcatc aatgccctta acccagccaa
     1921 tacgatcgcc tcgtccggtg aactcatatg gacggatcgg ttcaaatttg atccccgcgc
     1981 gacgctccac ttccgcttta aacgtctcaa cccccacgcg ctccagcgtg tatttggttt
     2041 tggcattttt acgatcggtt cggttacccc agtcacgctg agttgtcacg acggcttcgg
     2101 ccaccgccag cgtatgctcc agcggcagat agccaaactc gctcgccgtg cgggcgtagg
     2161 ttttcttgtt gccgtgttcg atagaaagcc caccgcccac cagcaggttg aagcctacca
     2221 gcttgccgtt ttcggcgatt gccacgaagt tcatgtcgtt ggcatgcaga tcgatatcgt
     2281 tctgcggcgg gatcactacc gtggttttga acttacgcgg caggtaggtc tgaccaagga
     2341 tcggttcttc atcagtagtg gcgacttttt cctggtcgag ccagatctcc gcatacgcgc
     2401 gggtacgagg caacagatgc tcagaaatct tcttcgccca ctcgtacgct tccgcgtgca
     2461 gctgcgactc gtaagggttc gaggtgcaga gtacgttacg gttcatgtcg ttagctgtcg
     2521 ccagcgcatc aagaccgacc gagtgcagca tctggtgcac cggtttgacg ttctttttca
     2581 gaatgccgtg gaactgaaac gtctggcggt tggtcaggcg aatgctgcca tagatggtgt
     2641 tttcaccggc aaatttgtcg atcgcctgcc actgtttagt ggtaatcacc ccacccggca
     2701 gacgacagcg aagcagcatc gcgtggcgcg gctccagctt ctgttcagca cgttcggcgc
     2761 ggatgtcgcg gtcatcctgc tgatacatgc cgtggaagcg gatcagcagg aagttatcgc
     2821 ccttaaagcc gccggtcaga ccgtcgttta aatcttccgc aatggtgccg cgcaggtagt
     2881 tgctttcaag cttcatgcgc tcggcgtctg tcagttttcc ttcgaccact aaaggccctg
     2941 gatgtttttc gctcattagt agacatctcg ctgataacgg cgctctacgc gcagctcact
     3001 taaaaattca tccgccgctt cggtgtccat gccaccaaat tcggcaatca cttccagaag
     3061 tgcctgctca acgtctttcg ccatgcgatt agcgtcgccg cagacataaa tgtgggcacc
     3121 atcattgatc cagcgccaca gctccgcgcc ctgttcgcgc agtttgtctt gtacgtaaac
     3181 tttttctttt tggtcgcgcg accaggcaag atcgatacgt gtcagcacgc catctttgac
     3241 gtagcgctgc cactccacct ggtacaggaa gtcttccgta aagtgcggat taccaaagaa
     3301 cagccagttt ttacctggcg cttcgtcggc ggcgcgttgc tgcataaagg cgcggaacgg
     3361 cgcaataccg gtgcctgggc caatcataat caccggggtt tctggattgg ctggtaggcg
     3421 gaagttatcg ttatgttcga taaatacgcg gacttcgccc tcttcttcca cgcggtcagc
     3481 gaggaagctg gaggcaccac cggcacgggc gcggccttcc acgtcgtaac gcaccacacc
     3541 aacggtgacg tgtacttcgt tctcgacttc cgcctgcgag gaggcaatgg aatagagacg
     3601 cggcgtcagc gggcgcagca gattaattag cgcttcggca tcaagctgtg ccggggagaa
     3661 acgcaccatg tcgacaatcg gcgtcgtcgc ggcgtaatgc tgtaacttcg ctttatcgcc
     3721 caccagcggc agcagtgttt cgctgcgggt aagcgtggcg taattctcaa caatgttggc
     3781 ggtgttgacg gtcagttcga agtgccactg tagcgcttcg ttcagaggca acgttttgcc
     3841 ctcgacggtg acaggttcat cgcctttcag ccacagcagt tcgacaagtt ctttcaccag
     3901 tgccggatcg ttctgatacc agacgcccag cgcgtcaccc ggctggtaac gcaggcccga
     3961 gtcacctaag tcaatttcga tatggcgaac gtctttttca gagttacgcc cggtaatttt
     4021 ctggttaaca gagaggctag ccaccagcgg cgcgtctttg ctgtacgggc tggtgtggat
     4081 ttcatttacc gcgccagtag cgacggattg cgaaggtgcc gcgacaggcg cacgcgattt
     4141 aagcgcatca accacgcggg cgcgccactc gctggcagca gcctggtatt caacatcggc
     4201 atcgacacgg tcgagcaggc gttcaccacc cagttccgcc agcttgctgt caaaatcttt
     4261 cccggactgg cagaaaaatt cataagagct atcgccgagg ctaaacacgg caaatgcggt
     4321 gttttccagc ttcggcgctt ttttggagaa caggaactta tgcagcgcga cggcttcttc
     4381 cggcggttcc ccttctcctt gcgttgacgt caccacgata agcagttttt cgctggcgat
     4441 ttgtttgaat ttatagtcgc ccgcgttcac cagcttaacg ttcagttttg ccgctaataa
     4501 atcatcacgt aatgcttcag caacacggcg cgcattgccg gtttgcgagg cggagataat
     4561 agttataccc ggcatttctg cggcgggagc tggcgtcgct gcaagcgcag caggctgctg
     4621 attgagcacg ccccagaaat agccagaaac ccaggcaagc tgggtgggag ttaaatcggt
     4681 cgtggccgcc tgaaggcgta ccagttgctc cgggttcaac ggaagcaacg cggaaggtgg
     4741 gacctgtgtc gtcatgcgtc gttatgttcc agtaagcaaa gctgtttctg cgccctgtca
     4801 gcgcccataa aacagaagag aaggtaaggt taacggggtg aacggtgtgg attaaagacg
     4861 ggatagcgat aactaataac caaatcgact aacctgtttt agcaatagtc tttaacaaca
     4921 aaatagatta accaacctaa tgaaaaacaa atgaatttag ccaatcatta agataaatca
     4981 gcgattttgc gcaacaaagt cgttttagat aatgcgaaaa aacagccttt ccggtactct
     5041 acggcggttt tatcgtcctg tagagaaatt atgatgtcca ccacgttatt taaagatttc
     5101 accttcgaag ccgctcaccg cttaccacac gtcccggaag ggcataaatg tggtcgcctg
     5161 cacgggcatt cctttatggt gcgactggaa attaccgggg aagtcgatcc gcatacgggc
     5221 tggattatcg atttcgctga actgaaagca gcgtttaagc caacttatga gcgcctcgat
     5281 caccattatc tcaatgatat tccaggcctg gaaaacccga ccagcgaagt tttagcaaaa
     5341 tggatttggg atcaggttaa acccgttgtg ccgctgttaa gtgcggtgat ggtaaaagaa
     5401 acctgcaccg ctggttgtat ctatcgcggc gaatgataag agtgtgtcgg cggtcaattt
     5461 cccttaagta acgctatgtt agggtgttgt gttctggata tctggggcat gacatggaag
     5521 acgactgcga cattattatt attggtgccg gtattgcagg caccgcttgc gcgttacgct
     5581 gcgcgcgagc gggtttatcc gttttgttac tggaacgcgc tgaaatcccc ggcagcaaaa
     5641 atctttccgg cgggcggtta tatacccatg cactcgcgga actcctccct cagtttcatc
     5701 tgaccgcgcc tcttgaacga tgcatcactc acgaaagcct ttccctgtta acgccggatg
     5761 gcgcaacgac gttttccagc ttacagcccg gcggtgaatc ctggagtgta ttacgtgcac
     5821 ggttcgatcc gtggctggtt gccgaagccg aaaaagaagg tgtcgaatgc atccctggtg
     5881 cgacggtgga tgcactgtat gaagaaaacg gcagggtgtg tggtgtcatt tgtggtgacg
     5941 atattctccg cgcccgttat gtggtgctgg cagaaggtgc caacagcgtc ctggctgagc
     6001 gtcacgggtt agtgactcgt cctgctggcg aagcgatggc gttggggatc aaagaagtgc
     6061 tgtcgctgga aacatccgct attgaagaac gttttcatct ggagaataac gaaggcgcag
     6121 cgttgctgtt cagcggcggg atctgtgatg acttacccgg cggcgcattt ctttatacta
     6181 atcaacaaac gctctcgtta gggattgttt gcccgctctc ttcccttacg caaagccgtg
     6241 ttccggcaag cgagctgctg actcgcttta aagcgcatcc ggcagtgcgc ccgcttatca
     6301 aaaacacgga atcactggag tatggtgcgc atctggtgcc agaaggtggc ttgcacagta
     6361 tgccggtgca atacgccggt aacggctggc tgctggtggg cgatgcgttg cgcagttgcg
     6421 tcaataccgg aatttccgtg cgcggcatgg atatggcgct gactggcgcg caggcggcgg
     6481 cacaaacgct gataagcgcc tgccagcacc gcgagccgca aaatctgttt ccgctttatc
     6541 atcacaacgt agagcgcagc ctgctgtggg atgttctaca gcgttatcag catgttccgg
     6601 cgcttttgca acgcccggga tggtaccgta cgtggcctgc gttaatgcag gatatttccc
     6661 gcgatttatg ggatcagggt gataaacctg ttccaccgct gcgccagtta ttctggcatc
     6721 atttacgtcg tcacggcctg tggcatctgg cgggcgatgt tatcaggagt ctgcgatgtc
     6781 tgtagcccgt aatctctggc gcgttgctga tgcgccgcac attgttccgg ctgactccgt
     6841 tgagcgccag acggcagaac ggttgattag cgcctgtccg gcaggtcttt tttcgctcac
     6901 accggaaggt gacttacgta ttgactatcg cagttgcctg gagtgtggca cctgccgttt
     6961 gctgtgcgac gaatcaacac tacaacagtg gcgctatccg ccttccggat tcggcatcac
     7021 ctaccgcttt ggataaataa taaggataat ttatgcccct cttacacctg ctccgccaga
     7081 atccggtgat tgctgccgtt aaagataatg ccagcctgca actggcaatc gattctgaat
     7141 gccaatttat ttccgtgttg tacggcaata tctgcaccat cagtaatatc gttaaaaaga
     7201 ttaagaacgc cgggaaatat gcttttattc atgttgattt gctggaaggc gcgtcaaata
     7261 aagaagtcgt tattcagttt ttgaaactgg tgaccgaggc ggacggcatt atcagtacca
     7321 aagcctcaat gttgaaagcg gcaagagcgg aaggtttttt ctgtattcat cgcctgttta
     7381 ttggtgattc aatttcgttt cacaacattg ataagcaagt tgcgcaatcg aatccggatt
     7441 gtattgagat cctgccaggc tgtatgccca aagtgctggg ctgggtgaca gagaaaatcc
     7501 gccaaccgct gattgccggt gggctggtgt gcgatgaaga agatgcgcgt aatgcgatta
     7561 acgcgggtgt cgtggcgctt tccaccacga ataccggggt ctggacgtta gcgaaaaaat
     7621 tactttgacg ggataatcgt aaccaattga atttggtttg atttgtaggc cgcacgccac
     7681 atccgacatt cagcgcctga tgcgacgctt gacgcgtctt atcaggccta caagtcccca
     7741 ggccagaacc gtaggcagga tcatccggca acacgttatt ggcaatctgc gtgaatgttt
     7801 gtcaccagtg cctccagcac cactttccag tcatcaacca cgccaacatc tgcctgtgaa
     7861 aataccgcag cgctggcgtc atgattaatc gccaccacaa acttgctatt gcgtacgccc
     7921 gccattaacg ctgctgcgcc agaagcgcca acaacaatac acaccttagg tgccagcaga
     7981 tgtccggaaa taccaatcac tttttcagca tcaacgccac cgttcatcac tcgcgcccga
     8041 ctgtagccca cttcggctcc cagcttttct gccagcatag cgatttcctg gttatctgcc
     8101 tctccgccct gccccaccac cagcacccgt ctggcctcag ctaatgggtc gcgagtaaca
     8161 tttttcagat cctctgtgct aaccagccag tccggaagcg cacccgggac aatgatcagt
     8221 tgttgcattc cagagggcaa cgtggcgttt ttcgctgctc ccgcctgacg cgccagggaa
     8281 aggcatagcg ggcgtttttc tgtttgcagc gtcgccgtta gcgcatttcc ccagtgggat
     8341 ttgcgcacgc ttacggttgg aatatctaac gaagtcacct ggcagatgct tgcaccatgt
     8401 aaacgccagg ccagccgggt cgacagttca tcgccaaacg ttcccggcgg aaagagaaca
     8461 acgtccgcag gcgttcgctg ccactgttcc accaacgcat ccagcacctg ttccgccact
     8521 acaggctgag gttcaatctg ccagtgcgcc agggtacaac cgctgaaatc ctgcgccgcc
     8581 agccagcttg ctattgcggc attctcctga tttattgtca caattgcgat attcatggct
     8641 gcatcctttg gcgtaaatag tcccgccata gcttttgagc tttttctgct accgtttgcc
     8701 catcaatcag cgtcgctccg cgtcgttgct ctgctcttgc cagttgcaga cactgcatcg
     8761 ctggcatttc ggcagcaaca gttttacgga taatttctgc tttccccgca gccattcgct
     8821 ggcgcattcc cggtactggc aaggccactt caccgcactg acgcacggca atcaccgcag
     8881 gcaagcgaac ccggcaacaa cgcaacccat gttcagtacg ctgttcgagg gtgataaaca
     8941 gcgcgtcgag agtgaaacgc tccacctggg tgaagcaggg ccagcccagc atttccgcca
     9001 gcaaaaatgg cgtttgccca ttctgccctt cgctgctttg gcagccggta attatcagat
     9061 ccagcggatt ctgatgctgc cattcggcaa tatggcgagc gacaaattcc ggcgcaaagc
     9121 gcagatctgc cgccgtctcc agcaatacag cttcctcaaa cccaagagcc atgagatagc
     9181 gcagccagtg caatgcccgt tcatccccca tgctcaacgc ggttaaagac atcggcgtac
     9241 cgttttttct ctgcgccaac agcagcgcgg cagcggcctg ttcatcagca ccgagtaaac
     9301 ttcgcagtag cgaaatatcc ggtccgcttt taccctgagc cgccgcctgc cactcttttt
     9361 ccgccagcat tccggcatcc ggttcggctt taaacgctaa cagaatgttc attcgcacct
     9421 cctacatttc ccgccgccac cagcgggagg gctttagttt ctggtgccca tagccaggtc
     9481 acaacaaaac caaccaacaa cactgtcgcc aggagcaata aggtgacttg cattccccac
     9541 tgcgccagca cccacggcag caggccagta cttaccgccg cgccaagtcg actcatggcg
     9601 gtggcaaaac cgacgcccag cgagcgaatg tcagtaggaa aactttccgc aggcaaaatg
     9661 cccaccagat tactgactgc cgaaatggtg gtgctgaaga gaacaaaaag cagcagcgtt
     9721 aatgaactgc cggaaggcaa acaggccatg actaccagcg ttgccgccag cagcaaaaaa
     9781 cttcccagca aaaatttgcg atgtgccagc aggtgcgtca gaactaatcc cagcagcgcg
     9841 cccacaatta acaacgcatt aagcatcagg ctggcagtca gcgcatcttc cagaccaata
     9901 gtctgggcga tagttggcag ccaggtataa atcacaaacc atgggattac gaggcagaca
     9961 aagaagacgc tgttaaacgc cgtgcgccgc cagtaacgcg aagagaacaa ggttttgatg
    10021 tgtttatggg tcgccgttac cacttcatcg cccagtaaaa catggggacc aaaatagcga
    10081 tgcacgatag cgtgagcttc tgcaaaacgc ccctggcgca gtagccagcg tggtgattct
    10141 ggcgttcccc agcgtaataa cgtaatcaac aacgcgggca gagctgccga tgccagtagc
    10201 cagcgccagg cctccgggtt ttcggaaata aagtgatgtc cggcaatact tgccagcaca
    10261 tagcctacgg tccacaccac gctgaatgcg cccagcaaaa taccgcgatg gcggcgcggg
    10321 gaaaattcag ccagcaaggt gtgacctact gaataatcgc ctcccagacc aatgccaatc
    10381 aaaatgcgca gtccaataag atgctctggc gtggtggcaa aaaattgtaa aaacgaagca
    10441 agcgtaatca gcaaaaagct gaaggtgaag attttttgcc gaccaatatg gtcggagatc
    10501 cacccaagaa cccggctgcc aaggaacaaa ccaaggagcg ccgagccgcc gatcattccc
    10561 gccataaacg gcgtcagttg catggcgggc gtaagctgaa taatggcgta accaatgacg
    10621 ccgagaacat aaccgtcggt caggtgtgcg ccgaaagtga gcgcagcaat gcggcagtga
    10681 aaacggttaa gcggtaaatc atccattcgc accggtgaag tgttcatctt taccccttca
    10741 ttgtccggta ataagaacgg cttctcgcct gagaagccga aaaggcaacg ccccgtcatc
    10801 gcggaacgct gcctgacaca ttatttttca atcggataga tagtacccgt attcataatg
    10861 ccattaggat cgaactgctt tttcagccct tccagcaacg cccacgcgct gccgtgttcc
    10921 agtttgctcc agtgaacgcg atgtttaccg ataccgtggt ggtgcaccat cgaaccgccg
    10981 aggcgaatgg tttcttcaca gatgatcttg ttgagcggat tgtggtactt gtcgatttcc
    11041 tcttccggct tacagtcaac gacgttgtaa tcgtagacga agtacatgtt ggtgccgttc
    11101 tgatagctat gagaggaatg accgcccagc atggtgatgt cgtcggcgtg cgggaactca
    11161 gtacgaatac ggttaataac gctttcgtag atttcgtgga tgcagctcca gcagccggac
    11221 acttcagtgg taaagcccat gttgccggtt ttgaggatct gcacacgttc ggcagccact
    11281 ttatccggtc cccagttcag gttgttaaac caggtttcga tcagtttgct gtccacgcgc
    11341 tggcactgcg ggtagcgggc aacgatttcc gcaatccctt cgcccgtcgc cttcgcaatg
    11401 cgaggattgc cttcagccat aaagatcagc acacatttac cgtcggcaaa atgggtgaag
    11461 tgttgggtgc catcttcagc gtcatacaaa cgagcgatcg acggacgata cccttccacc
    11521 atgacttcac gcaggatgtt gaagccggtt ttcatgtctt ccaggatata gccgtagaag
    11581 aggttgtttt ccggggtgaa tttaaagatt ttcactgtta cttcagtgat atagcaaagt
    11641 gcaccttcgt taccgatgat gatgtggcga atgtccgggc cagccgcgcg gcgtggcacg
    11701 tttttaatgc gtgtgacggt gccatcagcc aatactgctt ccagaccaac gaccatatct
    11761 tcgattgcgc cgtagagtgt ggagaactgc ccgatactgc gggttgctac caggccgccc
    11821 atctgcgcca gcggctttga ctgcggagaa tgccccgtgg tgtaaccttt ttcacgcaac
    11881 gcattttcca gcacctccag cggaacgcca cattgcgccg tcgcctgcat attctcaata
    11941 tcaatattaa tgatttgatt catggcggag ccgtcgagca ccaccgagtt ttccacaacg
    12001 gtttccagcc caccttcagt ggcggaagcg ccggtacgcg gcacaccgtt aattttgtgc
    12061 gcattcataa aattcagcac acgggagact tgttctgtgg aaccgagttt tacgaccgct
    12121 gccggaatcg gcaaagtata aataccatga atatccggaa atttacgaaa acggtcaata
    12181 ctgttcttct ttaatactgt ttcatcggta attacgcgat ctgcaccaac aatttccttt
    12241 agttggtcga caatcgctgc gcgagataaa gacatagtaa ttccttcctg ataataaaaa
    12301 tggaataatc attaataaga gaaaacagaa accaccgtta tgggttagcg cactaaataa
    12361 ccgccatcaa ccactaataa atgcccgttg acataattcg atgccggact tgcgaggaat
    12421 acggctgcgc ccattaaatc ctgagtatcg ccccaacggt ttgccggaat atgatcaaga
    12481 acgcgctgat tggtttctgg attactgcgt gtcgccagcg taatatctgt tgcataatag
    12541 ccaggggcga taccatttac ctgaatatta tattgaccta gttcatcaca ataagctttg
    12601 gtgaacccgg caagagcatg tttagtggca gaatatgcag gtgaccattg tccacctaag
    12661 taagagaaca atgaacagat attaataatt ttgccgcttt tctgcgggat cataattttt
    12721 gcagcttcat agcttaactc gaatgcggcg gtcaggttca catcaatcat cggatcccag
    12781 tcggcacgac cgaagtccag caccttattc agcttacaaa taccggcatt gttaaccaga
    12841 atatcaactg taccgaaacg ctcacagcta gcagcgataa tcttctgcgg cgcgccttct
    12901 gcggtgatat ccacctgcat gaagtccacc tcaacaccct gtttttcaat catttccttt
    12961 gtttcgccgt tatctttgac gaaactagga ataaagatat ttgcgccagc tttggccaac
    13021 gccatggcaa atgcctggcc taaaccgcta ttcccaccgg taacaattgc ggttttacct
    13081 ttcagggaga aaaaatccat tgagaacgca ttgagagatt cgattgacat agttagctcc
    13141 aatttccgta aggcaaaaaa aaagaaaggt aacttccccc gcgagaggga agttaccttt
    13201 ctcattatct ctggtaactg tagataattt ttagcataac ggcataactg ctaatgtgat
    13261 cgtaatcaca gtgtgatatt cgtattgtca aatagtttta aaaagcagaa gcgtgattac
    13321 tctcacacaa cacctctgct ttccatgtta tttcttattg caattactag agacctgaga
    13381 aaagtaatcc acctgaaagg gcggcttact tttctctttt ttttgtctat tacaaatggg
    13441 aatacgatat gcaacacaac tcatatcgcc gttggataac cctcgcgata attagtttta
    13501 gcggcggcgt tagtttcgac ctggcttatt tacgttatat ttatcaaatt cccatggcga
    13561 aatttatggg attcagcaat accgagatag gtttaataat gagtaccttt ggtattgcgg
    13621 ctattattct ttatgccccc agcggcgtta ttgccgataa attttcacac cgcaaaatga
    13681 ttacttccgc gatgatcatt accggattac tgggtctgtt aatggcaacg tatccaccgc
    13741 tctgggtaat gctctgtatt caggtcgcct ttgcgataac aacgatttta atgctgtggt
    13801 cggtgtcgat taaagccgcc tcgttgctgg gcgatcatag cgagcaaggg aaaattatgg
    13861 gctggatgga agggctgcgc ggcgtcggtg taatgtcgct ggcggtgttt accatgtggg
    13921 tcttttctcg ctttgcaccg gatgacagca ccagcctgaa aacggtcatt atcatctata
    13981 gtgtggttta catcttgttg gggattctgt gctggttttt tgttagcgat aacaacaacc
    14041 tgcgcagtgc caataacgaa gaaaaacagt cattccagct tagcgacatc ctggccgttt
    14101 tgcgcatcag caccacctgg tattgcagca tggtgatttt tggtgtcttc accatctacg
    14161 ccattctgag ttactccacc aactatctga ccgaaatgta cggcatgtcg ctggtggcgg
    14221 cgagctacat ggggattgtg atcaacaaaa tcttccgcgc gctgtgcggc ccacttggcg
    14281 gcattatcac cacctacagt aaagtgaaat cccctacccg cgtgattcaa atcctttcca
    14341 tcatcggttt gctggcgttg acagccctgc tcgtcacgaa ctctaacccg caatcggtcg
    14401 cgatggggat tggcctgatt ttactgctgg gattcacctg ttacacctca cgcgggctgt
    14461 actgggcctg ccccggcgaa gcgagaacac cgtcttacat tatgggcacc acggtaggta
    14521 tttgttcggt gattggattc ctgccggatg tcttcgttta cccgattatc ggccactggc
    14581 aagacaccct gcccgctgca gaagcctacc gcaatatgtg gctgatgggc atggcggcgc
    14641 tcggcatggt gattgtcttt acctttttgc tgttccaaaa aattcgtact gctgatagcg
    14701 cccccgcaat ggctagcagc aagtaagcca ggaaaattac aggagaaaaa tcatgtcgaa
    14761 aaaatacatc atagggattg atggcggaag tcagagcaca aaagtggtga tgtacgatct
    14821 ggaaggtaat gtggtttgcg aaggtaaagg cttattacag ccgatgcaca cgccagatgc
    14881 cgatactgca gaacatcctg acgacgattt atgggcatca ttatgttttg ccggtcacga
    14941 tttgatgagt cagtttgccg ggaataaaga agatattgtc ggtattggtc tgggatccat
    15001 ccgttgctgc cgtgcgttat tgaaagccga tggcacgcca gctgcgccgt tgattagctg
    15061 gcaggatgca cgcgttacac gcccttacga acatacgaat cctgacgtgg cgtatgtcac
    15121 ctctttttcg ggttatctga cgcatcgctt aaccggcgag tttaaagaca atatcgccaa
    15181 ctattttggt cagtggccgg tggattataa gagctgggca tggagcgaag atgctgcggt
    15241 aatggataag tttaatatcc cccgtcatat gctgtttgat gtgcaaatgc ctggcaccgt
    15301 cctcggacat atcacaccac aagccgcact ggcgacacat ttcccggcag gactgccggt
    15361 tgtttgtacc accagtgata aaccggtaga agctctgggg gccggattac tggatgatga
    15421 aactgcggta atttctcttg gcacttacat cgcattgatg atgaacggca aagcactgcc
    15481 gaaagatccg gtggcgtact ggccgattat gtcttctatt ccgcaaacat tgctgtatga
    15541 aggttacggt attcgcaaag gtatgtggac ggtgagctgg ctacgcgaca tgttaggcga
    15601 gtcgttaatt caggatgcca aagcacagga tctttcaccg gaagatttgc tcaacaaaaa
    15661 agcttcttgt gtgccgcctg gctgtaatgg tctgatgacg gtgctggact ggctgaccaa
    15721 cccgtgggaa ccctacaaac gtgggattat gatcggcttt gattccagca tggattacgc
    15781 atggatatat cgttcgatac tggaaagcgt ggcgctgacg ctgaagaaca attacgacaa
    15841 tatgtgtaat gaaatgaatt actttgcaaa gcatgtgatc attactggcg gcggttcgaa
    15901 cagcgatctg tttatgcaga tttttgccga cgtgttcaac cttccggcac gacgtaacgc
    15961 cattaacggt tgtgcaagcc tgggggcagc gattaataca gcggtaggtc tggggctata
    16021 cccggattac gcaacggctg tcgataaaat ggttcgcgtg aaagatatct ttatgcctgt
    16081 tgagagcaat gccaaacgct acgacgcgat gaataaaggc attttcaaag atttaaccaa
    16141 acacactgat gtgattctga aaaaatcgta tgaagtgatg catggggaat tggggaatgc
    16201 ggattcgatc cagagctggt cgaatgcgtg aatgaggtag gccaggcgga aaactgcccg
    16261 gcctgaacat acctaaatag ttatccccgc tgacgcgggg aacataagtt cgacaactta
    16321 aaaaatgata acaagtccgg tttatccccg ctgatgctgg gaacataata aaaactttaa
    16381 ctagttcggt ttatccctgt ttttacaagg aatattaact acactcgatg tctttagaat
    16441 aaaagacaat acgaatcgca ctctatcaat tttcacttaa agtatgaact ggatatcttt
    16501 attcataaga actattcatc aacagcgtat agaggcggtt atgtaaaacc actcattagc
    16561 ctcaaaacaa agacaaattt ccagatttat tacttaatca atgaataaca agcttaaaaa
    16621 atacactgtt attctaaaga aaaaattaac aggcattaat taata
//
LOCUS       DAJOWB010000103        16634 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJOWB010000103 DAJOWB010000000
VERSION     DAJOWB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.103"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            71..1210
                     /gene="arnB"
                     /locus_tag="OHO85_003597"
     CDS             71..1210
                     /gene="arnB"
                     /locus_tag="OHO85_003597"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCQ2672918.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPDWPHVHAWHLFIIRVDEQCCGISRNALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1214..2182
                     /gene="arnC"
                     /locus_tag="OHO85_003598"
     CDS             1214..2182
                     /gene="arnC"
                     /locus_tag="OHO85_003598"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCQ2672919.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2182..4164
                     /gene="arnA"
                     /locus_tag="OHO85_003599"
     CDS             2182..4164
                     /gene="arnA"
                     /locus_tag="OHO85_003599"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCQ2672920.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFTYVGNQKFTVWSSRVHPHASKAQPGSVISIAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYYVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4161..5051
                     /locus_tag="OHO85_003600"
     CDS             4161..5051
                     /locus_tag="OHO85_003600"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCQ2672921.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5051..6703
                     /gene="arnT"
                     /locus_tag="OHO85_003601"
     CDS             5051..6703
                     /gene="arnT"
                     /locus_tag="OHO85_003601"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCQ2672922.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPNFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFEIYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6700..7035
                     /gene="arnE"
                     /locus_tag="OHO85_003602"
     CDS             6700..7035
                     /gene="arnE"
                     /locus_tag="OHO85_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCQ2672923.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7035..7421
                     /gene="arnF"
                     /locus_tag="OHO85_003603"
     CDS             7035..7421
                     /gene="arnF"
                     /locus_tag="OHO85_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCQ2672924.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPMTKQRY"
     gene            complement(7415..7681)
                     /gene="pmrD"
                     /locus_tag="OHO85_003604"
     CDS             complement(7415..7681)
                     /gene="pmrD"
                     /locus_tag="OHO85_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCQ2672925.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7791..9146)
                     /gene="menE"
                     /locus_tag="OHO85_003605"
     CDS             complement(7791..9146)
                     /gene="menE"
                     /locus_tag="OHO85_003605"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCQ2672926.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGVRVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLASFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9143..10105)
                     /gene="menC"
                     /locus_tag="OHO85_003606"
     CDS             complement(9143..10105)
                     /gene="menC"
                     /locus_tag="OHO85_003606"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCQ2672927.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMTSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10105..10962)
                     /gene="menB"
                     /locus_tag="OHO85_003607"
     CDS             complement(10105..10962)
                     /gene="menB"
                     /locus_tag="OHO85_003607"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCQ2672928.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10977..11735)
                     /gene="menH"
                     /locus_tag="OHO85_003608"
     CDS             complement(10977..11735)
                     /gene="menH"
                     /locus_tag="OHO85_003608"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCQ2672929.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLTG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVVLRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11732..13402)
                     /gene="menD"
                     /locus_tag="OHO85_003609"
     CDS             complement(11732..13402)
                     /gene="menD"
                     /locus_tag="OHO85_003609"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCQ2672930.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSASLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13491..14786)
                     /gene="menF"
                     /locus_tag="OHO85_003610"
     CDS             complement(13491..14786)
                     /gene="menF"
                     /locus_tag="OHO85_003610"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCQ2672931.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNRRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14865..15170)
                     /gene="elaB"
                     /locus_tag="OHO85_003611"
     CDS             complement(14865..15170)
                     /gene="elaB"
                     /locus_tag="OHO85_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCQ2672932.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDTADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15225..15686)
                     /locus_tag="OHO85_003612"
     CDS             complement(15225..15686)
                     /locus_tag="OHO85_003612"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCQ2672933.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIHA"
     gene            15751..>16634
                     /gene="rbn"
                     /locus_tag="OHO85_003613"
     CDS             15751..>16634
                     /gene="rbn"
                     /locus_tag="OHO85_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCQ2672934.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPAT"
BASE COUNT         3837 a         4525 c         4265 g         4007 t
ORIGIN      
        1 ggcaaaatat acacaacact ctttatagca aatataagtg gacaggtatt caatggcgga
       61 aggaaaagca atgtcagaat ttttgccttt ttcgcgacca gcaatgggcg tggaggaact
      121 cgctgcagtt aaagaggttc tcgaatccgg ttggatcaca accggtccga agaatcaggc
      181 gcttgagcaa gccttttgcc agttgacggg aaatcagcat gccatcgcgg tcagttcagc
      241 caccgccgga atgcatatca cgctaatggc gttggaaatt ggcaagggcg atgaagtgat
      301 tacgccttcc ctgacctggg tttcaaccct caatatgatt tccttgttgg gtgcaacgcc
      361 ggtaatggtg gatgtcgacc gcgatacgct gatggtcacg cctgaagcga tcgagtcagc
      421 cattacgcca cgcactaaag ccatcattcc ggtgcattat gccggtgcgc cagcagatat
      481 tgacgccatt cgcgccattg gcgaacgtta cggcatcgca gttatcgaag atgctgccca
      541 tgccgtcggt acgtattaca aagggcgaca tattggcgca aaaggtaccg ctattttttc
      601 atttcatgcc attaaaaata ttacctgtgc ggaaggtggc ctgattgtaa ctgataatga
      661 aaaccttgcc cgccagctac ggatgctgaa atttcacggt ctgggtgtcg atgcctatga
      721 cagacaaacc tggggccgtg caccgcaggc tgaagtctta acaccgggct ataagtacaa
      781 tctgaccgat attaacgccg cgattgccct gacacagtta gtcaaattag agcacctcaa
      841 cacccgtcgg cgcgaaattg cccagcaata tcagcaagca ctggcagctc tcccctttca
      901 gccattaagc cttcccgact ggccgcacgt tcacgcctgg catctgttta ttattcgtgt
      961 cgatgaacaa tgttgtggta tcagtcgaaa tgcgttgatg gaagcgttaa aagaaagagg
     1021 cattggtacc gggttacatt tccgcgccgc tcacacacaa aaatattatc gcgagcgttt
     1081 tcccacgctg tcgttaccga ataccgaatg gaatagcgaa cgcatctgtt cgttgccgct
     1141 gttcccggat atgactaccg ccgatgccga ccgcgtcatc acagcccttc agcaactcgc
     1201 aggacaataa gccatgtttg aaatccaccc tgttaagaaa gtctcggtgg ttattcccgt
     1261 ttataacgag caggaaagct taccggaatt aatcaggcgc accaccacag cctgtgaatc
     1321 gttggggaaa gagtatgaga tcctgctgat tgatgacggc agtagcgata attccgcgca
     1381 tatgctggtc gaagcctcac aagcggagaa cagccatatt gtgtctattt tgcttaaccg
     1441 caattacggg caacattcag cgattatggc gggattcagt cacgttactg gcgacttaat
     1501 tattaccctt gatgccgatc tccagaatcc gccagaagaa atcccccgcc tggtggcaaa
     1561 agccgatgaa ggttacgacg tggttgggac tgtacgccag aaccgccagg acagctggtt
     1621 tcgtaaaacc gcttcgaaga tgattaaccg gcttattcag cgcaccactg gcaaagcgat
     1681 gggtgactac ggttgtatgc tgcgcgccta tcgccgtcat attgtcgatg cgatgttgca
     1741 ctgccatgaa cgcagcacct ttatcccgat tctggcgaat atcttcgccc gccgtgcaat
     1801 tgaaattcca gtacatcatg ccgagcgtga gtttggtgaa tccaaataca gttttatgcg
     1861 cctgattaat ttgatgtacg acctggtgac ctgccttacc acaacgccgc tacgtatgct
     1921 gagtctgctc ggtagcatta ttgcgattgg aggttttagc attgcggtgc tgctggtgat
     1981 tttacgcctg accttcggac cacaatgggc ggcagaaggc gtctttatgc tatttgccgt
     2041 gctgtttact tttattggcg ctcagtttat cggcatggga ttactcggtg aatatatcgg
     2101 caggatctac accgatgtcc gcgcccgccc ccgctatttt gttcagcaag ttatccgtcc
     2161 atccagcaag gaaaatgaat aatgaaaacc gtcgtttttg cctaccacga tatgggatgc
     2221 ctcggtattg aagctctgct ggctgccggt tacgaaatta gcgccatttt tacccatacc
     2281 gataatcccg gtgaaaaagc cttttatggt tcggtggctc atctggcggc ggaaagaggc
     2341 attccggttt atgcgccgga taacgttaat catccgctgt gggtggaacg cattgcccaa
     2401 ctgtcaccag aggtgatttt ctctttttat tatcgccatc ttatttgcga cgagattttg
     2461 cagctcgctc ccgcaggtgc atttaatctg cacggttcgc tgttaccaaa atatcgtggt
     2521 cgcgcgccgc tgaactgggt gctggtgaac ggtgaaacgg aaacgggcgt tacattgcac
     2581 cgaatggtaa aacgtgccga tgctggggcc attgtggcgc aactgcgcgt tgccattgcg
     2641 ccagacgata tcgctattac gctgcatcat aaattgtgcc atgccgcgcg ccagctactg
     2701 gaacagacat tacccgccat taaacacggt aatattctgg aaatcgccca gcgcgaaaac
     2761 gaagccacct gttttggtcg cagaacgccg gatgacagtt tccttgaatg gcataaaccg
     2821 gcatccgtac tgcacaacat ggtacgtgcc gttgccgatc cgtggccggg tgccttcacc
     2881 tatgttggca atcagaaatt taccgtctgg tcgtcgcgtg ttcatcctca tgccagcaaa
     2941 gcacagccgg ggagcgtgat ttctattgca ccgctgttga ttgcctgtgg cgatggcgcg
     3001 ctggaaatcg tcaccggaca ggcgggcgac ggcattacta tgcagggctc gcaattagcg
     3061 cagacgctgg gcctggtgca aggttcacgc ttgaatagcc agcctgcctg caccgcccga
     3121 cgccgtaccc gggtactcat cctcggggtg aatggcttta ttggcaacca tctgacagaa
     3181 cgcctgctgc gcgaagatca ttatgaagtt tacggtctgg atattggcag cgatgcgata
     3241 agccgttttc tgaatcatcc gcattttcac tttgttgaag gcgatatcag tattcattcc
     3301 gaatggattg agtatcatgt caaaaaatgt gatgtcgtct tgccgctggt ggcgatagcc
     3361 acgccgattg aatatacccg caacccgctg cgcgtatttg aactcgattt tgaagagaat
     3421 ctgcgcatta tccgctacta cgtgaagtac cgtaagcgaa tcatcttccc gtcaacttca
     3481 gaagtttatg ggatgtgtag cgataaatac ttcgatgagg accattctaa tttaatcgtc
     3541 ggcccggtga ataaaccacg ctggatttat tcggtgtcaa aacaattact tgatcgggtt
     3601 atctgggcct atggcgaaaa agagggttta cagttcaccc tcttccgccc gtttaactgg
     3661 atgggaccac gactggataa ccttaatgca gcgcgaattg gcagctcccg cgctattacg
     3721 caactcattc tcaatctggt agaaggttca ccgattaagc tgattgatgg cggaaaacaa
     3781 aaacgctgct ttactgatat tcgcgatggt atcgaggcgt tataccgcat tatcgaaaat
     3841 gcgggaaatc gctgcgacgg tgaaattatc aacattggca atcctgagaa cgaagcgagc
     3901 attgaggaac tgggcgagat gctgctggcg agcttcgaaa aacatccgct gcgccatcat
     3961 ttcccaccgt ttgcgggctt tcgcgttgtc gaaagtagca gctactacgg caaaggatat
     4021 caggacgtag agcatcgtaa accgagcatc cgcaatgccc accgctgcct ggactgggag
     4081 ccgaaaattg atatgcagga aaccatcgac gaaacgctgg atttcttcct gcgcaccgtt
     4141 gatcttacgg ataaaccatc atgaccaaag taggcttacg cattgatgtc gatacctttc
     4201 gtggcacccg tgaaggcgtg ccgcgtctgc tggaaatctt gagtaagcat aatattcagg
     4261 ccagcatttt tttcagcgtc ggcccggaca atatgggccg ccatctctgg cgactggtga
     4321 agccacagtt tttgtggaag atgctgcgct caaatgcggc atcgctttat ggctgggata
     4381 ttttactggc aggtacggcc tggccaggta aagagattgg tcatgccaat gccgatatca
     4441 ttcgtgaagc ggctaaacat cacgaagtcg gcctgcacgc ctgggatcac catgcctggc
     4501 aagcccgtag cggtaactgg gatcggcaaa caatgatcga cgatattgca cgcggtcttc
     4561 gcactctgga agagattatc ggtcaaccgg taacctgttc tgccgctgcg ggctggcgtg
     4621 ccgaccagaa ggtgatcgaa gcaaaagaag cgttccattt gcgctacaac agcgattgtc
     4681 gtggggccat gccgttccgt ccattgctcg aatcaggaaa ccctggcact gcgcaaattc
     4741 cggtgacctt acccacctgg gatgaagtga ttggtcggga tgtgaaagca gaagatttta
     4801 acggttggtt actcaaccgc atcctgcgag ataaaggcac gccggtttat accattcatg
     4861 cagaagttga aggctgcgct tatcagcata attttgtgga tctcctcaaa cgcgcagctc
     4921 aggaaggcgt gacattttgc cctttaagcg aactgttatc agagacgttg ccgctcggac
     4981 aagttgttcg cggaaatatt gccggacgtg aaggctggct gggttgccaa caaattgcgg
     5041 gtagtcgctg atgaaatcgg tacgttacct tatcggcctc ttcgcgttta ttgcctgcta
     5101 ttacctgtta ccgatcagca cgcgtctgct ctggcaaccc gatgaaacgc gttatgcgga
     5161 aatcagtcga gaaatgctgg catccggcga ctggattgtg ccccatctgt tagggctacg
     5221 ttatttcgaa aaacccattg ccggatactg gattaacagc attgggcaat ggctatttgg
     5281 cgcgaataac tttggtgtgc gggcaggcgt tatctttgcg accctgttaa ctgccgcgct
     5341 ggtgacctgg tttactctgc gcttatggcg cgataaacgt ctggctctac tcgccacagt
     5401 aatttatctc tcattgttta ttgtctatgc catcggcact tatgccgtgc tcgatccgtt
     5461 tattgcattc tggctggtgg cgggaatgtg cagcttctgg ctggcaatgc aggcacagac
     5521 gtggaaaggc aaaagcgcag gatttttact gctgggaatc acctgcggca tgggggtgat
     5581 gaccaaaggt tttctcgccc ttgccgtgcc ggtattaagc gtgctgccat gggtagcgac
     5641 gcaaaaacgc tggaaagatc tctttattta cggctggctg gcggttatca gttgcgtact
     5701 gacggttctc ccttggggac tggcgatagc gcagcgagag cctaacttct ggcactattt
     5761 tttctgggtt gagcatattc aacgctttgc actggatgat gcccaacata gagctccgtt
     5821 ctggtactac gtgccggtca tcattgccgg tagcctgccg tggctgggat tactccccgg
     5881 tgcactgtac acaggctgga aaaaccgcaa gcattccgca accgtctatt tgttgagctg
     5941 gacgataatg ccgctgctgt ttttctccgt cgctaaaggt aaattgccca cctatattct
     6001 ttcctgcttt gcatctctgg caatgctgat ggcgcattac gctttgctgg cagcaaaaaa
     6061 taatcctctg gcgctgcgga ttaatggctg gattaacatc gcttttggcg tcactggcat
     6121 tattgccaca tttgtggtct ccccctgggg accaatgaac acgccggtgt ggcaaacctt
     6181 cgagatctat aaagtctttt gtgcctggtc gattttttcg ctatgggcat ttttcggctg
     6241 gtacacctta acaaacgtcg aaaagacctg gtcttttgcc gcgctttgcc cgctggggct
     6301 ggcgttgctg gtaggatttt caattcctga cagagtcatg gaaggaaaac atccgcaatt
     6361 ttttgtcgag atgacacaag aatcacttca gccaagccgc tatattctta ctgatagcgt
     6421 cggtgttgcc gcaggtctgg cgtggagcct gcaacgcgat gacatcatca tgtatcgcca
     6481 gacaggtgag ttgaaatacg gccttaatta tccggatgcg aaagggagat ttgtcagcgg
     6541 tgatgagttc gcaaactggc ttaatcaaca tcgtcaggag gggattatta cactcgtgct
     6601 ttcggttgac cgcgatgaag atatcaacag tctcgccatt ccgcccgcag atgccatcga
     6661 tcgtcaggag cgtctggtgc tgattcagta tcgtcccaaa tgatctggct aacattagtc
     6721 tttgccagct tgcttagcgt tgccgggcag ttgtgtcaga aacaggcaac ctgctttgtg
     6781 gcgataaaca aacggcgcaa acatatcgtg ctgtggctgg gactggcgct ggcttgtctt
     6841 ggtcttgcca tggtgctctg gctgctggtc ttgcagaacg taccggtagg cattgcctac
     6901 ccgatgttaa gtctgaattt tgtctgggtg acgctggctg cagtaaaact gtggcacgaa
     6961 ccggtatcac cgcgtcactg gtgtggggtg gcgttcatta ttggtggcat tgtgatcctc
     7021 gggagtacgg tgtaatgggc ctgatgtggg gattattcag cgtcattatt gcctcagttg
     7081 cgcaattaag cctgggtttt gcggcgagtc atctgccgcc gatgacgcac ttatgggatt
     7141 ttatcgcggc tctgttagct tttggcctgg atgcccggat actgttactc gggctgctgg
     7201 gttatctgct ttccgttttt tgttggtata aaacgctaca taaacttgcg ctaagcaaag
     7261 cctatgcctt gctcagtatg agttatgtgc tggtatggat tgcttcaatg gtacttcccg
     7321 gctgggaagg gactttttcg ctgaaagcac tactgggagt agcctgtatt atgagcgggt
     7381 tgatgctgat tttcctgccc atgacaaaac aacgttactg agttttccct gccactttac
     7441 actgccgttc ccactcatcg gggctataac tgcttgccga gaggactttc accgtatgca
     7501 gcttttcacg attaatacaa taaagcgcat tctgcaaagg cgagagtaaa tcccccactt
     7561 tcacggcgaa gtcactcttc acttcggcga tcatttttat cgcgccgcca gcatcgcaga
     7621 gcataagtac atgtctgtta tcttgtttat tgcagcacga ttttttgacc agccattcca
     7681 ttgcattatc ctgtttgcta agagttttca catcaattta cagattaaca gataaactct
     7741 ttatcaacgg caatggaatg gcagaaatga gaagtgaaac ggagggttaa ttattgctga
     7801 cgttgcaccc actcttttag cgcctgacgt gaaattttaa taccgccgtt tttcagttca
     7861 ggcggcaacg ttaaccaacg caccggttgc tgaaaactgg ccagcttatc tttcacccat
     7921 tcactaagat caacgctctc gtggtcatac tccatcaccg ccaccggtcg atgaccaaac
     7981 tccttgtcgg caacggggac gataaacacc tgcaaaaccg caggatgtgc agcaataaca
     8041 cgctcgactt cttccggctg aataccctct ccgccactga agaatagatt gtctaaacgt
     8101 ccgacaatgg tcagcttgcc attatgcatc tcaccgcgat cgcgcgtagc gtaccagcct
     8161 tcgtcattaa ccagtgaaac cagttgcccg ttacgccagt aaccttctgc catactggca
     8221 gcccgcagcc acacttcatt attaacgatt ttcacttccc gacccggcag cggcgaacca
     8281 acgtctgcca ggccgtcggc ttctttcgca cacaccgtgg aggcaaactc ggtcagacca
     8341 tagccgcaaa agcaacgaat cccctgctcg cgcgcctgtt ccgtcaactc gaccgggata
     8401 gccgcgccgc caagtaacac cgctttcagg gaaacggaac tgcggttaac cagcaaacgc
     8461 cagagttgtg ttggcaccag tgaagcatga gtacagcctg ccagcatttg ctccaatggc
     8521 tgtttatcac gcaccgtcat ccgcgcacca gcgtataacc agcgccacat aattccctga
     8581 ccggagacgt gaaacagcgg taaagagagc aaccagtcat cgtgatcgcc aaacggaatc
     8641 aacgataaca ccccttcggc actggcaaga tgggcctgat aagtatggac agcggctttc
     8701 ggcaacccgg tagaacccga ggtcaacgtc attgagcaca gacgcgtcgg ctgccacgta
     8761 gcggcatgtg cgccttcaac ccgctgaatg tgcagcgacg ttaacgccgg aaacgtgttt
     8821 tccccatccg gcaccagcgc aaattgcagc gtcagattgg gcagcaattc ttcaagcaac
     8881 ggttgcggca gctgagggtt cacgggcaac acccgcaccc cgcattgcag taacgccagc
     8941 caggcgagca gcgtttgcgg cgtattccac gcccgcaaca tcacgccgct gccctcaaca
     9001 acgccctgcg ccgcaaagcc cgtagctaac tcatcgacgc gagcacaaag ctcgcgccag
     9061 ttaagttgct cgtcattaag acgtaaggcg atggcttctc cccgcacttg ccgccagtga
     9121 cgccacggcc agtcagagaa gatcataaca accgctccag tgcatcaact tccacgacag
     9181 gcagcgtgct acccggccag cgacgtacct gctgcgcctg catcagatcc agcgtgtcca
     9241 gccctggaat ggtgtccggc gttaaccagg cggcaatccg cgccagttgc gttaagccta
     9301 agctcgattc aatggaagaa ctgatcaccg ccgtcagccc cagcgcgtgc gccgcctgta
     9361 cctgctcgcg tactttctcc agactgcccg tgagcgtggg tttgataact accgcgcgca
     9421 cgccctcttc agccacaaag gcaaaatccg gctcgcgcaa gctttcatcc caggcaatgg
     9481 caatgccggt ttcacgggca aacgctcgcg aatcatcgcg ggttttgcac ggctcttcga
     9541 gaaacgcgat gcggtggcga taatccgggt taacatattt ggcaaactgc tggcctttca
     9601 gcggtgtcca ggcgcgattt gcgtcaagac gcaaatgcag atccggaatt gcctccagca
     9661 acagattaac caccatgccg tcgcgcacgg cttcgtacaa tcccactttg accttcgcca
     9721 ctttctcgcc tggcatatca gcaagtttga ggatcagatc gtcaggatcg ccattacaca
     9781 gtggtgccgc acggtaattg gccgcctgcg ggagcgtatc tgccagctcc gccaatgcac
     9841 agcttacgcc aaaggcaacg gaagtcatct gcggtagctc gcaatcgcct gccagccagt
     9901 tatttaccca ggcaagcagc acgctttgcg cctcttccca ggtttcctgg ctgaagcccg
     9961 gcagtgggga gatctccccc cacccttcgc gctcgccctc acgcaggcaa acgtacagcc
    10021 cgtcgcgggt ttttaaccgc ctgtcgcgca gaaccacccc cgcgtccatg gggatctgcc
    10081 agcggtatac ctgcgcgcta cgcattacgg attccgttta aatttgctga aatcaggctg
    10141 acgtttctgg ttgaaagcgt tgcgaccttc ctgaccttct tccgtcatgt agaacagcat
    10201 ggtggcgttg cccgccagct cctgcagccc cgcctgcccg tcacagtcgg cgttcagcgc
    10261 agctttcagg cagcgcagcg ccatcgggct gttttgcagc atttcgcggc accaacggac
    10321 ggtttctttt tccagatccg ccagcggtac cacggtgttg acaaggccca tatccagcgc
    10381 ctgttttgcg tcgtactgac ggcacaggaa ccagatttct cgcgcttttt tctgcccgac
    10441 gatacgagcc atgtaggaag cgccccagcc gccgtcgaag gaaccgactt tcgggccagt
    10501 ctgaccgaag atggcattat ctgctgcgat agtcaggtcg cacatcatgt gcagaacgtg
    10561 accaccgccg atggagtagc cagccaccat cgcgacaacc ggtttcggac aggtacggat
    10621 ctgacgctgg aagtccagca cgttcaagtg atgtacgccg gaatcatctt tatagccgcc
    10681 gtaatcacca cgcactttct ggtcaccacc ggagcagaac gctttatcgc ctgcgccagt
    10741 cagaatgatc acgccgatat tgtcgtcata acgcgcatct gccagtgcct ggatcatctc
    10801 ttttaccgtc agaggacgga aggcattgcg cacctgcgga cgattaatgg tgatttttgc
    10861 gataccgtcg gtggattttt cataacgaat gtcctcgaaa ccttcggagc agtcgtgcca
    10921 ttcaaccggt gcgtaaagca ttgcttcatc aggataaatc atattgtgtc ctttagtcag
    10981 aaacgcaaga tctgcgccag acttgcgatt acgccagcgg gattttcccg atgcgcgtta
    11041 tgtccggcgc gaggaatgac atggcagtcg gcagccagtt ccgccgccag ggcgcggaat
    11101 ttgctgtcac gttcaccaca taaataataa aacgcaaatg tgcgggcgct aaggttagca
    11161 cgtaaatcag gctggacggc gagagaagtc gcctccagca tggcggcaag ggttgcgcca
    11221 ttattgttgc tgcgcagcac caccagctcc cggcgttgat cgtcattgag cgaggcaaaa
    11281 acaggctgtt gataccagtc ggcaaatacc gccgttaacg gttctgtgca aaaacgctgc
    11341 gcccattggc gatcggaacg ctgacgttcc gcacgttgtt cagcattttg cagccccgga
    11401 tgcccgcctt cgacaataac cccacaaagc cccgtcagcc cctggcaagc cgccatcatc
    11461 gccacccgtc cgccaagcga gtaccccacc agccagaagt caaggatgtt gtaactaacc
    11521 aaggttttac gcagtaagtc ggtgacatca tcaaatccat cgacgctaat cgccgccgaa
    11581 ccaccatgac ctgggagatc aacatacaac cgtgagtagt cggcaaacgc ctcgcccact
    11641 tcttgccatt cgtggcaatc gccggaaaaa ccgtgcaaaa acaccagcca gggtaaacct
    11701 ggttttccgt gttttgcctg cgcgtgcagg atcataaatg gcttacctgc gccagaagtt
    11761 gctggagcgt ttgcgcacca tcggtgtcgt taaccaccat ttcaatcacc gtggtggttg
    11821 gcgtgcgcca ggcgtcggca aatgccgttt caagttcctg ccagttttgc ggacgatgat
    11881 atttcagctc gaacatcgcg gcggcgtgct caaaatggac gttttgcggc atcagataga
    11941 aacgctcacg ctcgctttgc ggcgttggca acagcgagaa aatttgcccg ccgttgttgt
    12001 tcaccacaat taataccagc gacgcagaaa cctgacgcaa taacgccagc gcgttgagat
    12061 cgtaaagtgc ggagagatcg cccacaatcg ccagcgtcgg tttgccgctt gcccgctgaa
    12121 cgcctgcggc ggtcgaaagc agtccgtcga taccgctggc ccctcggttg ctgtacaccg
    12181 ggtaacctgc cggaagttgc gaaagcgcat caatcagacg taccaccagg ctgttaccaa
    12241 caaacaattg cccctgttca ggcagatagt cgcagatgcg atgcgccagt tgcgcttcgc
    12301 caaacgcatc acggcgggca ataaccgcct gcattgcctg ttccgccagg cgcgggattt
    12361 caacgcacca gggctggcgt ttttctgccg gatgcagctc cagccagtcg gcaatattgg
    12421 caattaagcg acgtccgcga tggtgtgccg gatcaagtcg cccttcaatg tcatcaacaa
    12481 tccagtactc ttctggttca cagcttgcct gccattgcag gagccgtttc cccgtcaggc
    12541 tgcttcccag ttgcaccaca atttgcgcct gctgcagctc gctggtcgct ttggcattgc
    12601 ctaaccaaag atcggcacac ggcagcggct gcccggtttg tgacagcaca tcgccaatca
    12661 gcggccagcc aagagtttgc gcccacaggg caactttttt gccctcttcc gcactcatgc
    12721 gcccggcaac caccacgccg cgcttttgtc gccagaagaa ccagtcgcgc tgtttttcac
    12781 tttccagacg aggcgcttca cgcagccacg gtttgtcgtc ctgccaccag tcacccagac
    12841 gctgttgcca gctaagcccg gtatcgtcca tttcgccata cagcggttca gcaaacgggc
    12901 agttgatatg gactcccccc gcatgaagcg taccgagagc gtggtcgatg gtagaaacca
    12961 gccaacgtgc ggggatatcc tgggtcgggc gcggcaatga aatactgtgc gtggggtgag
    13021 aggcgaacat tcccggctgg cgaattgcct gattcgcgcc gcagtcaatt agctccggcg
    13081 ggcgatcggc ggttaagaga atcagttttt ctccggttaa cccggcttca atcagtgccg
    13141 gatagagatt tgccaccgcc gtgccggagg tcacaatcac cgccaccggc tgcttgctga
    13201 ctttcgccag ccccagcgcc agatgcccca acccacgctc atcgaaatgg gtgtggtgaa
    13261 tgaatgcgga attctccgcc gccgctaacg ttaacggtgt agaacgcgag cctggggcga
    13321 tacagatgtg tctgacgccg tgacgcgtta atgcttccag aatgaccgcc gcccagcgtc
    13381 ggttaaatgc gcttactgac atgagtttgt ccggtatcaa tattgcggct aagtataagg
    13441 agctacaaaa atagattatt gatatgaatc ggtaatgatg cgactcatta ctattccatt
    13501 tgtaataaag tacgcagccc tgccgctttg ttgtcgattt cctgccactc ttgctcgggg
    13561 tcggaaccac ggacaatgcc cgcgccagca tataatcgca cgacattgcc gctaattttt
    13621 gctgagcgca gggaaacgca gaattcgctt tgttgtaatg agagatagcc cgcagaaccg
    13681 gcgtaccatt ctcgggtgaa cggttcgtga cgggcgataa actgtcgcgc cagatcgcgc
    13741 ggtaagccag caactgctgc cgttggctgc aactgatgta aacagatcac atcatccgct
    13801 ttgttgagtg aagtccagat acagcggcga agatgctgca ctttacgcag acgcagtacc
    13861 tgcggcggta aaacatccag cgtctgggta tcggcctgta atcgttggca gatatcttcc
    13921 accaccagca tgttctcgcg ccggttttta tcatccgcca tcagccactc tcctaactgc
    13981 tgcgcctgct tatcatcagg attatttgct actgttcccg ccagcgcttc agtacgcagc
    14041 gctttgtcac gccgccgcca taaccgttcc ggtgaagagc caagaaaagc attttcgcca
    14101 tcaaaggcca tgtaaaaatg gtagcaattc agattcagtc gacgactggc agccatcatc
    14161 gccgccgcgt tgaccggact tgcgaaatgc aggtcagttg cccgagcgag caccactttg
    14221 tcgagctcac cttcggcgat ggttttcgtt gccagttcga ttaattgcgt ccagcccgtt
    14281 ttgtccggcc agtgttgttc tcgcgtggtg gttaaatgta acccaggcaa gggcttgata
    14341 ctcaccagtg tggcgataaa ttcttttgcc tgaatcgcat cgtgctgaag ggagctttcg
    14401 ctgaatagcg tcagccgcag cgtggcttta ccgccacagc gtcgccattc caggcggggt
    14461 aaaagtaaat tgccctgcga cgggtcaaaa gcattcagcc cccaaatgcg taagtcggcg
    14521 tgttccgggt gctggcgaag aaaacgttgt gcctggtcca acgacgtaaa acgggtaatc
    14581 gcgcccagga cggcagcttc ttcatcacca ttacgttgtt gccagtagaa ttgcgggtac
    14641 gtttgctgac tggccagcca gctcaaggca tcaaaagcgt ctttgagagg gaaaggaata
    14701 tcgataaccc gaatgccggg tgtcgccgga atctcttgcg acaaatggcg cagtagattt
    14761 tccagcgccg tagtaagtga ttgcacgcgg acctctccct gttaaaagcc tcacattata
    14821 cggggtacta caaaaaaaat gcagtacccc ggtgtaggga ggttttaacg gcgtgccagc
    14881 aacagtccta gtaccagccc aacggccgcg cccacaccaa ttccttgcca gggtttttcg
    14941 tggacgtagt catcagcacg ataaactgcc tgcttcgccc gatagtaata actgtctgaa
    15001 gcctggctaa cccgtttttt cacatcatcc agcgcttttt ctgcacgcgc tttcagctca
    15061 acatatttct gatcggcggt atcgccagag gagcggagca cctcttccag tgtttcacta
    15121 agcagcgtca ggtcgtcatc gatacgtgta tcaccaaact gattagacat tctcgttctc
    15181 catttgcgta aaacctgtca ctaactatag acaagggttg ttgattacgc atgaattacc
    15241 tcgcgtgcca tcccaatgtg tgggatacca tcttcttcat agacctccgt cacagggata
    15301 aaaccgaaac tctggtagaa gttttgcaga tgcgcctgcg ccccgaggta aacaggctta
    15361 tcaggccagt gatgcgtaca tgtttcgagt gttttactca tcagctgctg ccccactttt
    15421 tcgccacgca acgcttcact gacaatcacc cgacctataa cgaccggctc aagatcatca
    15481 tcacttttca gaatcctcgc atatgccacc agttcatcat ttttccaacc gagaatatgg
    15541 cgattatccc ccgtcaaatc atcaccatcg atatcctgat aagggcaatt ctgctcgaca
    15601 acaaacaccg cgcaacgcag ttgtaataag gcataaagct gggaaaccga taattcagag
    15661 tgatgcagat cttgccattc aatcataatg ctctcctttt agatgtatgc cttatactag
    15721 ccggaatatt ttttgaaacg tgatgaactc atggaattaa tttttttagg tacttcggcc
    15781 ggtgtgccaa cccgcacgcg caatgtcacg gcaatattgc ttaacctgca acatccgacc
    15841 cagagcggtc tttggttgtt tgactgcggt gaaggcaccc agcatcagct actgcatacc
    15901 gcctttaacc ctggaaaact ggacaagatt tttatcagtc accttcatgg cgatcatctt
    15961 tttggtttac ccggcttgct gtgcagtcgt tctatgtcag gcattatcca acccttaacg
    16021 atttatggtc cgcaaggtat ccgtgaattt gtggaaaccg cgctgcggat tagcggctca
    16081 tggaccgatt atccgctgga aattgtcgaa attggcgctg gcgaaattct cgatgatggc
    16141 ctgcgcaaag taaccgctta tccgctggaa cacccactgg aatgttatgg ctatcgtatt
    16201 gaagaacatg ataaaccggg tgcattaaat gcccaggcat taaaagctgc tggcgtgccg
    16261 cctggcccac tgtttcagga attaaaagcg ggcaaaacaa tcacgctgga agatggaagg
    16321 cagattaacg gcgcagatta cttagctgct ccagtgccag gtaaagcgct cgctattttc
    16381 ggcgataccg gcccctgcga tgccgcactt gacctggcta aaggtgtcga tgtcatggtg
    16441 cacgaagcga cgctggatat aaccatggaa gccaaagcca atagtcgcgg ccatagctct
    16501 acacgccagg ctgcgacact agcccgtgag gctggagtcg gtaagctaat cattacccac
    16561 gtcagctcgc gctatgatga caaaggttgt cagcacctgt tacgtgaatg caggtcaatt
    16621 ttcccggcga ctga
//
LOCUS       DAJOWB010000104        16444 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJOWB010000104 DAJOWB010000000
VERSION     DAJOWB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.104"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            111..1442
                     /locus_tag="OHO85_003614"
     CDS             111..1442
                     /locus_tag="OHO85_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2672935.1"
                     /translation="MKLTINEKIGFGAGDMAIAIVMMSLAMIITYFYTDVFGLKPVDL
                     GILLFSVRILDAVIDPVVGTITDRTNTRWGKYRPWLLFMSIPFGISIWLMFTTPDTDY
                     SVKLLWAWATYVLLTLTYTLIAIPYVSLISVITDDPQERLSANGYRFVMTKIAMFAVT
                     IIVPLSAMYLGKNNVKLGYQIAMGAMGILATCLCLYCFFSVRERIYHPKPSLGMAAQF
                     RLLIKNDQWLILGAVIAIIMFGGIIRNSVAAYYAKYYLNGGDALISPFLTSGVIASVL
                     AMIACTWLTRLYDKIKIFRYTQLLAFVVGGAMYFVQPNSIVLAFTFYIVVTFLTDIQL
                     PIYWASIAESVDYGEMKTGQRVSGLAFGGILFFQKFGMGLAGGFIGIALAYLDYQPGV
                     EQTPQALWGICLLMTIFPAILNLITGLIMRFYLINNEFYEQIKARLQTAEE"
     gene            complement(1486..1800)
                     /locus_tag="OHO85_003615"
     CDS             complement(1486..1800)
                     /locus_tag="OHO85_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCQ2672936.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1831..2412)
                     /gene="mdaB"
                     /locus_tag="OHO85_003616"
     CDS             complement(1831..2412)
                     /gene="mdaB"
                     /locus_tag="OHO85_003616"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCQ2672937.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            complement(2522..3871)
                     /gene="qseC"
                     /locus_tag="OHO85_003617"
     CDS             complement(2522..3871)
                     /gene="qseC"
                     /locus_tag="OHO85_003617"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCQ2672938.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNRLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(3868..4527)
                     /gene="qseB"
                     /locus_tag="OHO85_003618"
     CDS             complement(3868..4527)
                     /gene="qseB"
                     /locus_tag="OHO85_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCQ2672939.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPDKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            4679..5071
                     /gene="ygiW"
                     /locus_tag="OHO85_003619"
     CDS             4679..5071
                     /gene="ygiW"
                     /locus_tag="OHO85_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCQ2672940.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            5124..5606
                     /locus_tag="OHO85_003620"
     CDS             5124..5606
                     /locus_tag="OHO85_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCQ2672941.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            5811..6107
                     /gene="mqsR"
                     /locus_tag="OHO85_003621"
     CDS             5811..6107
                     /gene="mqsR"
                     /locus_tag="OHO85_003621"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin MqsR"
                     /protein_id="HCQ2672942.1"
                     /translation="MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCN
                     VIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQVYLKITVIHDVLIVSFKEK"
     gene            6109..6504
                     /gene="mqsA"
                     /locus_tag="OHO85_003622"
     CDS             6109..6504
                     /gene="mqsA"
                     /locus_tag="OHO85_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MqsA"
                     /protein_id="HCQ2672943.1"
                     /translation="MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESI
                     MNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKKLSLTQKEASEIFGGGVNAFSRY
                     EKGNAQPHPSTIKLLRVLDKHPELLNEIR"
     gene            6637..8244
                     /locus_tag="OHO85_003623"
     CDS             6637..8244
                     /locus_tag="OHO85_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCQ2672944.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDQWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKALLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQTPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            8382..10640
                     /gene="parC"
                     /locus_tag="OHO85_003624"
     CDS             8382..10640
                     /gene="parC"
                     /locus_tag="OHO85_003624"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCQ2672945.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            10874..11611
                     /gene="plsC"
                     /locus_tag="OHO85_003625"
                     /gene_synonym="parF"
     CDS             10874..11611
                     /gene="plsC"
                     /locus_tag="OHO85_003625"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCQ2672946.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            11686..13098
                     /gene="ftsP"
                     /locus_tag="OHO85_003626"
     CDS             11686..13098
                     /gene="ftsP"
                     /locus_tag="OHO85_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCQ2672947.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            13209..15428
                     /locus_tag="OHO85_003627"
     CDS             13209..15428
                     /locus_tag="OHO85_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCQ2672948.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(15471..15728)
                     /gene="yqhH"
                     /locus_tag="OHO85_003628"
     CDS             complement(15471..15728)
                     /gene="yqhH"
                     /locus_tag="OHO85_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCQ2672949.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(15779..>16444)
                     /locus_tag="OHO85_003629"
     CDS             complement(15779..>16444)
                     /locus_tag="OHO85_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3828 domain-containing protein"
                     /protein_id="HCQ2672950.1"
                     /translation="FGDLVLESVAITQTDADHADAVVRFRIFKDDKEKTTQTLKMVAE
                     NGRWVIDDIVSNHGSVLQAVNSENEKTLAALASLQKEQPEAFVAELFEHIADYSWPWT
                     WVVSDSYRQAVNAFYKTTFKTANNPDEDMQIERQFIYDNPICFGEESLFSRVDEIRVL
                     EKTADSARIHVRFTLTNGNNEEQELVLQRREGKWEIADFIRPNSGSLLKQIEAKTAAR
                     LKQ"
BASE COUNT         3952 a         4087 c         4264 g         4141 t
ORIGIN      
        1 ttgttgtggt atctaccaat gcatttattc tcactcgtaa agttatgtgc tgaatgagtt
       61 ccgatacatt tgtttttaat ataatttcac gtgatcaatg agtgctgtgt atgaaactga
      121 caataaatga aaagataggt tttggtgccg gtgatatggc tattgcgata gtaatgatgt
      181 ctttagccat gatcatcact tatttttaca cggatgtatt tggtctgaaa cccgtcgact
      241 taggtattct gttattttct gtacgtatcc ttgatgctgt gattgatcca gttgtgggaa
      301 caatcacaga cagaacgaat acacgttggg gaaaatatcg gccgtggttg ctttttatgt
      361 caataccgtt tgggataagc atttggttga tgttcaccac acctgatacc gattattcag
      421 ttaagctttt atgggcctgg gctacctatg tgttacttac cttgacctat acgttaatcg
      481 ctattcctta tgtttcatta atcagcgtta tcacggatga tccacaagag cgattgagcg
      541 ccaatggtta tcgttttgtt atgacaaaaa ttgccatgtt cgccgtaaca attattgttc
      601 ctttatctgc aatgtatctt gggaagaata acgtaaaact gggttatcaa atagcaatgg
      661 gggcaatggg aattctggca acgtgtttat gtctgtattg tttttttagc gtgagagagc
      721 gcatctacca tccaaaaccc agtctcggta tggccgcgca gtttcggtta ctgatcaaaa
      781 atgatcaatg gctcattttg ggcgctgtaa ttgcgatcat tatgtttggc gggattattc
      841 gtaactctgt cgctgcatat tatgcaaaat attacctgaa tggtggggat gctttaattt
      901 caccgttttt aacttcaggc gtgatcgcct ctgttctggc tatgatcgct tgtacctggc
      961 ttactcgact ctacgacaaa ataaaaatat ttcgttatac ccaattactg gcgttcgtgg
     1021 tgggtggtgc gatgtatttc gtgcaaccaa attctatagt gttagctttt acattttata
     1081 ttgtggtgac cttcctgacg gatattcagc ttcccattta ttgggcatcc attgctgagt
     1141 ccgtggatta cggtgaaatg aaaacggggc aacgtgtttc tggcctggca tttggcggga
     1201 ttctcttttt ccaaaagttc ggtatggggc tggcaggggg ctttatcgga atcgctttag
     1261 cctatttgga ttatcaacct ggtgtagaac aaacaccaca ggcgctgtgg gggatctgcc
     1321 tgttaatgac gatctttccc gcgatactta acctgattac cggactcatc atgcgatttt
     1381 atctgattaa taatgaattt tacgaacaga ttaaagcacg gttacagacg gcggaagagt
     1441 gaatataaat ggccggcaaa cagcaccggc ctcttttcag gaatgttaaa tccctggctg
     1501 cagaatacga atattcatct ccagcacgtc accttttacg gcttcgctat acgccttcat
     1561 gtgcggggtt tgcagatgcg cttcaagatg cgcgatgctt tcccactgct ctatcatcac
     1621 gatagaatcc ggtgccatag actggaaact cacgccagca gcgcaatcca ccattggcgc
     1681 atagccgtgg caaccttctt ctttcagtac ggttggaacg attttagcaa actgatccaa
     1741 taccgcctga cggtgatgtt gaccaggacg agtacggatt tctgcgatta cggtaagcat
     1801 ggttaactcc ttctaaagcc tgagctctag ttaaccaaaa atttccacaa gatgcttgcg
     1861 atattcttca gtatagcggg gaacatcagg catttttatc acgtcattag cgataaatgt
     1921 cggcagcggt tccataccga ggaattggtt tgctttatgg aacggcagat acacgccgtc
     1981 aacgccaacg ccgtggaaga actgatcttt ttcggtgaag gcttccattg gtgcgttcca
     2041 ggtcagagaa agcatatatt ttttgccctg taccaggccg ccggagccgt attttttcga
     2101 cggatctttg cgggtacgac catcgctggc atacagagtc ccgtgacctt cggtgaatac
     2161 atcatcaatg tattttttca ctgtccacgg cgcgcccatc caccagcctg gcatctgcca
     2221 gatcaccaca tcagcccaga gaaagttttg tacttccgct ttgacatcgt agtcgctgtc
     2281 ggcgcgaacg atgcggacat catgcccgag gtcgcgcagt gtgccatccg cgacttcggt
     2341 cagggtgtcg ttcagttgac cattggagtg ggcgaatttt ttcgcgccgt tgataatcag
     2401 gatgttgctc attttttaac ctcaaggtga gacgtttgtc gactatagta cgtcacagag
     2461 cggtacggtg aaattagcaa aatgtgcaaa gtcttttgcg aatttagcaa aagtcttacc
     2521 cttaccagct taccttcgcc tcaaatccac cttgttccgc attcccaaac tcaacattca
     2581 tgccatgcag cttggcgatt cgctggacaa tcgatagccc aagtccactg ccggtggcgg
     2641 tttgtccggg tgggcgatag aagcgttcgc caattcgcgc cagtgcctct ggtgtcacac
     2701 cggggccgtt atccctcacg atgaaattat cggcattcag cgtgacgtcc accacgctgc
     2761 cctgtggact gtaacgcacg gcgttatcca gcaaatttcg caccaacaaa cttagcaata
     2821 gcggttgtcc ggtgcgtttg atgccctgga cattgagtgt cagtcgcacg tcaattttcg
     2881 cctgctgcgc cgtgtggtaa atatccatca ccgacgattg caggagatct tcaagcggga
     2941 tctccgcgac gtcctgaagg ttatccagtg agtccagccg cgatagcgtg agcagttgat
     3001 caaccagacg agtagcgcga tcgatcccgg aatgtaattg aagcagtgct tttttccgcg
     3061 cctgcggatc atcgtcagag agctgcgcaa cttcggtttg cactttcagc gccgttaacg
     3121 ggctacgaag ttcgtgagct gcgtcggagg taaagcgtcg ttcacgaacc atcatcgcat
     3181 gtgtgcgggc gaacagttga tttagcgact caaccagtgg ccgcacttcg ctgggtacgc
     3241 cagtcgcgtt tagtggtttt tccgagtcag ggtcacgcat acgtagtgcc agcgccagtt
     3301 tgttcagcgg cgcgagttca cgacccagta gtaccatcat gatgattaac ataatcggca
     3361 gtgcgaccag ccacgggatc aattgcccgg caacaatcgc cagcgccatg tcttcacggt
     3421 attcccattc ctggccaaca acgatgcgat atttgccatc aggtgaggtc atccagacaa
     3481 aacgccaagg atcgtcttca ccgaccagtt gcccgtcagc aaaaccttcc cgttgatagc
     3541 tatagggaat atcttctccg ttatcgccat cattaaggac cattctgccg tcgtgggtaa
     3601 agatggcaaa ggtcagcgca tcgtcatcaa catgaccgtg ttttaatcta tttggcgtct
     3661 gtgccatgcg atccgccgcg ttgatttcgt tgagatcgag cgtacttaac cgcttggcaa
     3721 acagcatcag ttgggtgtcg aacaattcat cgacgttatc cgttgtttgt ttccaggcga
     3781 caaagctgga aagcagccag gtcaccgagg ccagaattaa aaagattagc gtcagcctga
     3841 cgcgcagact aagacgttgg gtaaatttca tttctcacct aatgtgtagc caataccatg
     3901 cacggtacga ataaaatcac taccgagttt gcgtcgcaga tgatgcacat gcacttcaac
     3961 ggcattactg gtgacctctt cgtcccaggt atacagtttc tcttcaatca gtttgcgcgg
     4021 cagtacccga ccagcgttac gcatcagtaa ttccagcagg gcaaattctt ttggtttcag
     4081 tgttaagggt tcgccagcca gcgtggcgat acgtttgtcg gggtcgagca tgacgttacc
     4141 gtggcgcagc tcgttgctgg cctggccgtt ggttcggcgc atcagagctt ccagcctggc
     4201 ggcgacttct atcaacgcaa aaggtttaca cagataatcg tcagctccca gacgcagccc
     4261 ttctacacgt tccgccagcg catcgcgcgc ggtcaggatc agtaccggct cacgctgacc
     4321 tttttctcgc cattcgcgca aaatatcgcg accatccatg cctggtaagg ttaaatccag
     4381 gatcaccgca tcataaggtg cgctataaag cgcctctttt ccctgacgac cttgtgtaaa
     4441 ccagtcgacg ctaaaaccca ttttactaag gcccgttttg atgccgtcgc caatcagcat
     4501 gtcatcttct atcagtaaaa ttcgcatttt ttcatccctg cgataaccgt atttgctgcc
     4561 agtaaaccgt gttgtaacgc gctctgtaca gcactaaatt ttattttttt cccttaagaa
     4621 gttgttaagg actatcttgt taaatgctcg gaaacagaca ttaaagggag taataaacat
     4681 gaaaaaattc gcagcagtaa tcgcagtaat ggccctgtgc agcgcaccgg tgatggcagc
     4741 agagcagggc ggtttttctg gcccatcggc aacgcaaagt caggccggag gattccaggg
     4801 gccgaacggc agcgtaacga ctgtagaaag cgcaaaatcc ctgcgtgacg acacctgggt
     4861 aaccctgcgc ggcaatatcg ttgaacgcat ctctgacgat ctctacgtgt tcaaagatgc
     4921 cagcggtact atcaatgttg atatcgatca caaacgctgg aacggcgtga cggtgacgcc
     4981 gaaagatacg gttgagattc agggtgaagt cgataaagac tggaattctg ttgaaattga
     5041 cgtcaaacag atccgcaaag taaatccgta attgttactg ctcccgggac gcgttcccgg
     5101 gaataatttc gcagggaggc aaaatgacaa acctgacact ggatgtaaac attatcgatt
     5161 tcccatcaat ccctgtggcg atgttgccgc accgctgtag ccctgagttg ctcaactaca
     5221 gtgtggcgaa atttatcatg tggcgtaaag agacggggct ttctcctgtt aaccaaagcc
     5281 agacttttgg cgtcgcctgg gacgaccctg ccaccaccgc accggaagcg tttcgctttg
     5341 atatctgcgg cagcgttagc gaaccgattc ccgataatcg ttatggtgtg agcaatggtg
     5401 aacttaccgg tggacgttat gccgtggccc gccacgttgg cgagctggac gatatttcac
     5461 acacgatatg gggcattatt cgccactggc tgcctgcaag cggcgagaaa atgcgtaaag
     5521 caccgattct gtttcactac accaatcttg ccgaaggggt gacagagcag cgactggaaa
     5581 cggatgttta tgtgccgtta gcgtgatgcc tgactccagc ttcccttata aattcctgct
     5641 tcaaactggc gctactaatg cgccactttc ttatatggtc aatcactttc cttaaagaat
     5701 acgttttgtg tggtcactat atccgtacat ctaactaacc ttttaggtgg tttttcattg
     5761 ctgtaattaa ccttttaggt tataactaaa gtaacaggga ggcgggggtt atggaaaaac
     5821 gcacaccaca tacacgtttg agtcaggtta aaaaacttgt caatgccggg caagttcgta
     5881 caacacgtag tgcactgtta aatgcagatg agttaggttt ggattttgat ggtatgtgta
     5941 atgttatcat tggattatca gagagcgact tttataaaag catgaccacc tactctgatc
     6001 atactatctg gcaggatgtt tacagaccca ggcttgttac aggccaggtt tatcttaaaa
     6061 ttacggtaat tcatgacgta ctgatcgtct cgtttaagga gaagtaatat gaaatgtccg
     6121 gtttgccacc agggagaaat ggtttctggc attaaagata ttccatacac cttccgtgga
     6181 cgaaaaacag tattgaaagg tatccacggt ttatattgtg tccattgcga agagagcatc
     6241 atgaataaag aagagtcaga tgctttcatg gcgcaagtaa aggcatttcg ggcttcggtg
     6301 aatgccgaaa cagtggcacc tgaatttata gtgaaggttc gaaaaaagct ctctcttacc
     6361 caaaaagagg caagcgaaat ttttggggga ggtgtaaatg cgttttcgcg ttacgaaaaa
     6421 ggcaatgccc aacctcatcc ttccacaatc aaacttttac gtgttctgga taagcatcca
     6481 gaactattga atgaaatccg ttaattaatg gaaaagcggg ggcaactcgc ttttttcttt
     6541 accggagtgt gagccaaatc actcgcgata agtttgagcg aacaatgcca caggcgtatt
     6601 gttgttgggc ttataatttc tccggtaagg aataccatgt atacgcgaaa tttattatgg
     6661 ctggtcagcc tggtaagtgc ggctcctctc tacgctgctg acgttcccgc caacacaccg
     6721 ctcgccccgc aacaagtctt tcgttacaac aatcatagcg acccaggtac gctcgacccg
     6781 caaaaggtgg aggagaatac tgccgcgcag attgtgctgg atctgtttga aggtctggta
     6841 tggatggacg gtgaaggcca ggtgcagccc gctcaggctg aacgctggga gatactggac
     6901 ggcggcaagc gctatatttt ccatctgcgt agcggtttgc agtggtccga cgggcagcct
     6961 ctgacggcag aggattttgt cctcggctgg cagcgcgcgg ttgacccgaa aacggcaagc
     7021 ccttttgctg gctatctggc acaggcgcac attaacaatg ccgcagctat tgttgcgggt
     7081 aaagcagatg ttacatcgct gggtgtcaaa gcgacggatg atcgtactct tgaagttacg
     7141 cttgagcagc cggttccttg gttcacgacg atgctcgcct ggccgacgct gttcccggtt
     7201 cctcatcatg tcatcgctaa acatggcgat agctggagta agccagagaa catggtttac
     7261 aacggtgcct ttgtgcttga tcagtgggta gttaacgaaa agattactgc acgcaaaaat
     7321 ccaaagtacc gcgatgcgca acatacagta ttgcaacagg ttgagtatct ggcgctagat
     7381 aattcggtca ccggctataa ccgctatcgc gcgggagagg tcgatctcac ctgggttccg
     7441 gcgcagcaaa ttcccgccat tgaaaaatca ctgcctggcg agctacgaat tattccgcgt
     7501 ctgaacagcg aatattacaa cttcaacctt gagaaaccgc catttaacga tgtgcgagtg
     7561 cgtcgggcgc tatatcttac ggttgatcga cagcttattg cgcaaaaggt actggggttg
     7621 agaacgcccg caaccacgct gacgccgcca gaggtaaaag gctttagcgc gacgacgttc
     7681 gatgaactgc aaaagccaat gagtgagcgc gtcgcgatgg caaaagcctt gctgaaacag
     7741 gcgggatacg acgcctctca tccgctacgc tttgagctgt tctacaacaa gtacgatctg
     7801 catgaaaaga ccgcgatagc gttgtcttcc gaatggaaaa aatggctggg tgcacaggtg
     7861 acgctgcgca caatggagtg gaaaacctat cttgatgccc gacgagccgg tgatttcatg
     7921 ctgtctcggc agtcgtggga tgcgacgtac aatgatgctt ccagcttcct gaacacgctc
     7981 aaaagcgata gtgaagaaaa cgtcggtcac tggaaaaatg cgcagtatga cgccttacta
     8041 aaccaggcca cgcagatcac tgatgcgaca aagcgtaatg cgttatatca gcaggcagaa
     8101 gtgatcatca accaacagac accgctgatt cctatctact atcagccgtt aatcaaactg
     8161 cttaaaccct acgttggcgg ttttccgctg cataatcccc aggattatgt ctacagcaaa
     8221 gagttgtata tcaaggcaca ttgatgcctt tatcgccgtc ggagcacgtc cggcggcgct
     8281 attcacgctg atctcctgtg actcgacgcg gcagataatg tagtatctcc ggcaatattg
     8341 cccctttgaa ggctggcgaa taagttgagg aatcagaatt aatgagcgat atggcagagc
     8401 gccttgcgct acatgaattt acggaaaacg cctacttaaa ctactccatg tacgtgatca
     8461 tggaccgtgc gttgccgttt attggtgatg gtctgaaacc tgttcagcgc cgcattgtgt
     8521 atgcgatgtc tgaactgggc ctgaatgcca gcgccaaatt taaaaaatcg gcccgtaccg
     8581 tcggtgacgt actgggtaaa taccatccgc acggcgatag cgcctgttat gaagcgatgg
     8641 tcctgatggc gcagccgttc tcttaccgtt atccgctggt tgatggtcag gggaactggg
     8701 gcgcgccgga cgatccgaaa tcgttcgcgg caatgcgtta caccgaatcc cggttgtcga
     8761 aatattccga gctgctattg agcgagctgg ggcaggggac ggctgactgg gtaccaaact
     8821 tcgacggcac tttgcaggag ccgaaaatgc tacctgcccg tctgccaaac attttgctta
     8881 acggcaccac cggtattgcc gtcggcatgg cgaccgatat tccaccgcat aacctgcgtg
     8941 aagtggctca ggcggcaatc gcattaatcg accagccgaa aaccacgctc gatcagctgc
     9001 tggatatcgt gcaggggccg gattatccga ctgaagcgga aattatcact tcgcgcgccg
     9061 agatccgtaa aatctacgag aacggacgtg gttcagtgcg tatgcgcgcg gtgtggaaga
     9121 aagaagatgg cgcggtggtt atcagcgcat tgccgcatca ggtttcaggt gcgcgcgtac
     9181 tggagcaaat tgctgcgcaa atgcgcaaca aaaagctgcc gatggttgac gatctgcgcg
     9241 atgaatctga ccacgagaac ccgacccgcc tggtgattgt gccgcgttcc aaccgcgtgg
     9301 atatggatca ggtgatgaac cacctcttcg ctaccaccga tctggaaaag agctatcgta
     9361 ttaaccttaa tatgatcggt ctggatggtc gtccggcggt gaaaaacctg ctggaaatcc
     9421 tctccgaatg gctggtgttc cgccgcgata ccgtgcgccg ccgactgaac tatcgtctgg
     9481 agaaagtcct caagcgcctg catatcctcg aaggtttgct ggtggcgttt ctcaatatcg
     9541 acgaagtgat tgagatcatt cgtaatgaag atgaaccgaa accggcgctg atgtcgcggt
     9601 ttggccttac ggaaacccag gcggaagcga tcctcgaact gaaactgcgt catcttgcca
     9661 aactggaaga gatgaagatt cgcggtgagc agagtgaact ggaaaaagag cgcgaccagt
     9721 tgcagggcat tttggcttcc gagcgtaaaa tgaataacct gctgaagaaa gaactgcagg
     9781 cagacgcgca agcctacggt gacgatcgtc gttcgccgtt gcaggaacgc gaagaagcga
     9841 aagcgatgag cgagcacgac atgctgccgt ctgaacctgt caccattgtg ctgtcgcaga
     9901 tgggctgggt acgcagcgct aaaggccatg atatcgacgc gccgggcctg aattataaag
     9961 cgggtgatag cttcaaagcg gcggtgaaag gtaagagcaa ccaaccggta gtgtttgttg
    10021 attccaccgg tcgtagctat gccattgacc cgattacgct gccgtcggcg cgtggtcagg
    10081 gcgagccgct caccggcaaa ttaacgttgc cgcctggggc gaccgttgac catatgctga
    10141 tggaaagcga cgatcagaaa ctgctgatgg cttccgatgc gggttacggt ttcgtctgca
    10201 cctttaacga tctggtggcg cgtaaccgtg caggtaaggc tttgatcacc ttaccggaaa
    10261 atgcccatgt tatgccgccg gtggtgattg aagatgcttc cgatatgctg ctggcaatca
    10321 ctcaggcagg ccgtatgttg atgttcccgg taagcgatct gccgcagctg tcgaagggca
    10381 aaggcaacaa gattatcaac attccatcgg cagaagccgc gcgtggagaa gatggtctgg
    10441 cgcaattgta cgttctgccg ccgcaaagca cgctgaccat tcatgttggg aaacgcaaaa
    10501 ttaaactgcg tccggaagag ctacagaaag tcactggcga acgtggacgc cgcggtacgt
    10561 tgatgcgcgg tttgcagcgt atcgatcgtg ttgagatcga ctctcctcgc cgtgccagca
    10621 gcggtgatag cgaagagtaa tatgccgggg agggcgctgt ttcctcccgc ttgcaaggaa
    10681 cgccggatga aacgttaagt cgtcatccgg cagcgtattc aacccccgga aacgataaaa
    10741 attcccttga aaccgttgtt tattcatgcg ttgcgattaa caatacgctt ttccagagag
    10801 cggcttttaa caatgcccta acctgatttc aggtgacgta caatgccagt ctctcagacc
    10861 ttcagagggt gttatgctat atatctttcg tcttattatt accgtgattt acagcatctt
    10921 agtctgtgta ttcggctcca tttactgcct tttcagcccg cgtaacccga aacatgtggc
    10981 cacctttggg catatgtttg gccgtcttgc gccgctgttc ggcctgaaag ttgagtgccg
    11041 caaacctgca gatgctgaaa gctacggcaa tgctatctat atcgctaacc accagaacaa
    11101 ctatgacatg gtgacagcat cgaacattgt gcaaccgccg acggtgacgg taggtaaaaa
    11161 gagcttgctg tggatcccct tcttcgggca gttgtactgg ttaaccggca acttattgat
    11221 cgacagaaac aatcgcacta aagctcacgg caccattgcg gaagtcgtga atcatttcaa
    11281 aaaacgccgt atttccatct ggatgttccc ggaaggaacc cgcagccgtg gtcgcggcct
    11341 gctaccgttc aagactggag catttcacgc ggcaattgcg gcgggcgtcc cgattattcc
    11401 cgtgtgcgtc tctacaactt cgaataagat taatcttaat cgactgcaca acggtctggt
    11461 gattgtcgaa atgctgccgc caattgacgt cagtcagtat ggcaaagatc aggttcgtga
    11521 gctggctgcc cattgtcgtt cgataatgga acaaaaaatc gccgagctcg ataaagaagt
    11581 cgcagaacgc gaagccgccg gaaaagttta agtcggcaat ctgtattttt gcggggaaca
    11641 ctttcctgca cggtattact ttagccagtt ttacatggag caaatatgtc actcagtcgg
    11701 cgtcagttca ttcaggcatc ggggattgca ctttgtgcag gcgctgttcc cctgaaggcc
    11761 agcgcagccg ggcaacagca accgctaccc gttccgccgc tgcttgaatc tcgccgtggg
    11821 caaccgctgt ttatgactgt acaacgtgcg cactggtcat ttacgccagg gacacgcgcg
    11881 tcggtctggg gaatcaatgg tcgttacctg gggccgacta tccgcgtctg gaagggcgac
    11941 gatgttaagc ttatttacag caaccgcctg acagaaaatg tctcaatgac ggtagccggg
    12001 ctacaggtac caggcccgct gatgggcggt ccggcacgga tgatgtcgcc aaacgctgac
    12061 tgggcacccg tactgcctat tcgccagaac gcagctactc tgtggtatca cgccaatact
    12121 cccaaccgca cggctcagca ggtctataac ggccttgccg gaatgtggct ggtggaagat
    12181 gaagtcagca agtcgctgcc tatccccaac cattatggtg tggatgattt tccggtcatt
    12241 attcaggata aacggctgga taactttggt acgccagaat acaacgaacc gggaagcggc
    12301 ggctttgttg gtgatacgct gctggttaac ggtgtacaaa gcccgtacgt tgaagtctcg
    12361 cgtggctggg tgcgcttgcg cctgctgaac gcgtcgaact ctcgtcgtta tcaactacag
    12421 atgagcgatg gtcgcccgtt acatgtgatt tctggcgatc agggattcct gcctgctcct
    12481 gtatcggtga agcaactttc gctggcaccg ggcgagcgcc gcgagattct ggtggatatg
    12541 agcaacggcg atgaagtgtc gatcacctgt ggcgaagcgg cgagcattgt tgatcgtatt
    12601 cgtggcttct ttgagccatc cagcattctg gtttctaccc tggtgctaac gctgcgccca
    12661 accggccttc tgccgctggt cacagacagt cttccgatgc gcttgctgcc aactgaaatc
    12721 atggccggtt cgccgattcg cagtcgcgat atcagtctgg gtgatgaccc gggtattaat
    12781 ggacaactgt gggacgtcaa ccgtattgat gtcaccgcgc agcaaggaac gtgggaacgc
    12841 tggacggtac gcgcggacga gccgcaagcg ttccatattg aaggcgtaat gttccagatc
    12901 cgtaacgtga atggcgcgat gccgttcccg gaagacagag gctggaaaga taccgtttgg
    12961 gttgacggac aagtggagct gcttgtttat ttcggtcagc cttcctgggc gcacttcccg
    13021 ttctacttca acagtcagac gctggaaatg gcggaccgtg gctcgatagg gcaacttttg
    13081 gtcaatccgg taccgtaatc ttcatactcg cccgaggagg gcgagtatgg aatacctttc
    13141 gatttctctt tcgttccggc ttataatccc cggcctttga ttattttttt atcatttctg
    13201 gaagcaaaat gagctctatc tccctgatcc aaccggatcg cgacctgttc tcctggccgc
    13261 agtactgggc cgcctgtttt ggaccggcac cgtttttgcc gatgtctcgt gaagagatgg
    13321 atcaacttgg ctgggatagc tgcgacatca ttttggttac tggcgacgcg tatgtcgatc
    13381 acccaagctt cgggatggcg atttgcggtc gtatgctgga agcacagggc tttcgcgtcg
    13441 ggatcatcgc ccagccagac tggagcagca aagacgattt catgcgtctg ggtaaaccga
    13501 atctgttttt cggtgttact gctggcaaca tggattcgat gatcaaccgt tataccgccg
    13561 atcgccgttt acgtcatgac gatgcctaca cgccggataa cgtcgcgggt aagcgcccgg
    13621 atcgcgccac actggtttat acccagcgtt gtaaagaggc gtggaaagat gtaccggtga
    13681 tcctcggcgg tattgaggct agtctgcgcc gtaccgcaca ttatgattac tggtccgata
    13741 ccgtgcgccg ttccgtgctg gtggattcga aagccgacat gctgatgttt ggtaacggtg
    13801 agcgtccgct ggtggaggtg gcgcaccgtc tggcgatggg cgagccgatt agtgaaatcc
    13861 gcgatgtgcg taataccgcg attatcgtga aagaggcgct gcctggctgg agcggcgtgg
    13921 attccacccg tcttgatacc cctggaaaaa tcgacccaat cccgcatccg tatggtgaag
    13981 atttgccgtg cgcggataac aaaccggtgg caccgaaaaa gcaggaagcc aaagccgtaa
    14041 ccgtgcagcc accgcgcccg aaaccgtggg aaaaaaccta cgtgttgctg ccttctttcg
    14101 agaaagtgaa gggcgataaa gtgctgtacg cccatgcttc gcgcattctg caccacgaaa
    14161 ccaacccagg ctgcgcccgc gcattgatgc aaaaacacgg tgaccgctat gtgtggatca
    14221 acccgcctgc tatcccgctt tctaccgaag agatggacag cgtttttgcg ctgccgtaca
    14281 agcgcgtgcc acatccggcc tatggcaatg cccgtattcc ggcttacgaa atgatccgtt
    14341 tttcggtcaa cattatgcgc ggctgctttg gcggctgttc tttctgttct attaccgagc
    14401 acgaagggcg cattattcag agccgttcgg aagattcgat cattaatgag atcgaagcga
    14461 tccgcgacac cgttccaggt tttacgggcg tgatttccga tctcggtggg ccaactgcca
    14521 acatgtatat gttgcgctgc aaatcgccac gcgctgaaca aacttgtcgc cgtttgtcgt
    14581 gcgtttatcc ggatatttgt ccgcacatgg acactaacca cgaaccgacg atcaacctct
    14641 atcgccgtgc gcgtgatctg aaaggcatta aaaaaatcct catcgcctca ggcgtacgtt
    14701 atgacatcgc cgtggaagat ccgcgctata tcaaagagtt ggcgacccat cacgtcggcg
    14761 gttatctgaa gattgccccg gaacataccg aagaagggcc gttatcgaag atgatgaagc
    14821 cgggcatggg cagctatgac cgctttaaag agctgttcga tacttactca aaacaggcag
    14881 gtaaagagca gtatctgatc ccctatttca tctccgcgca ccccggtacg cgtgatgaag
    14941 atatggtgaa tctggcgctg tggctgaaaa agcatcgttt ccgtctcgac caggtacaga
    15001 acttctaccc atcgccgctg gcgaactcaa ccaccatgta ttacaccggg aaaaacccgc
    15061 tggcgaagat tggttataag agtgaagacg tcttcgtacc gaagggcgac aagcagcgtc
    15121 gtttgcataa agcgttgttg cgttaccacg atccggcaaa ctggccgtta atccgccagg
    15181 cgctggaagc gatgggcaaa aagcatctga ttggcagccg tcgcgattgc ttagtgcctg
    15241 cgccaaccat tgaagagatg cgtgaagctc gtcgccagaa ccgcaatacc cgtccggcgt
    15301 tgacgaaaca tacgccgatg gcgacccagc gtcagacgcc tgctacggca aaaaaagcgt
    15361 cgtctacgca atctcgtccg gtgaatgctg gtgcgaagaa acgccctaaa gcggcggttg
    15421 gacgttaaaa aaaatccccg gcaactgaca tgctgccggg gattttttta tcattctgcg
    15481 tacatacgca agcagcgcag gcaatcaaaa tagtcctgag catcaagacg cgtattggct
    15541 ctgttagctt cggatttggc agcatagatt tgtgggcgta gtgctttcat atcctgctca
    15601 agccgggcga ttttggcatt taatgtttgc acattgctcg ccagctgatt gaccttttgc
    15661 tcattgacgc agccactgag caagcaggtt gccagaacaa cagctcccac ggtgaaaatc
    15721 gttttcatat agtgttccct tttatttatt gttccggcta attgttattt aattcagctc
    15781 attgctttaa tctggcggcg gtttttgcct caatctgctt aagtaggctg ccgctgttcg
    15841 ggcggataaa atcagcgatt tcccacttgc cttcgcgccg ctgtaaaacc agttcttgct
    15901 cttcgttgtt gccattggtc agcgtaaaac gaacatgaat gcgggcggaa tcggcggttt
    15961 tctccaggac tcgaatttca tcaacgcgtg aaaatagcga ctcttcgcca aaacagatcg
    16021 gattgtcgta aataaattgc cgttctattt gcatatcttc atcgggatta ttggccgtct
    16081 tgaaggtggt tttatagaag gcattaaccg cctggcggta agagtcggaa accacccacg
    16141 tccacggcca gctataatca gcaatatgtt caaagagttc ggcaacaaag gcttccggct
    16201 gttctttttg caacgaagct aaagcggcca gcgttttttc attctcgcta ttaactgctt
    16261 gtaagacgct gccatgattg ctgacaatat cgtcaatgac ccaacgacca ttttccgcca
    16321 ccattttcag tgtctgcgtg gtcttttctt tatcatcttt aaagatacga aagcgcacaa
    16381 cggcatcggc atgatcggcg tcagtttggg ttatcgcaac gctttctagc accagatcgc
    16441 caaa
//
LOCUS       DAJOWB010000105        16182 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJOWB010000105 DAJOWB010000000
VERSION     DAJOWB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.105"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            315..1367
                     /locus_tag="OHO85_003630"
     CDS             315..1367
                     /locus_tag="OHO85_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HCQ2672951.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(1682..2884)
                     /locus_tag="OHO85_003631"
     CDS             complement(1682..2884)
                     /locus_tag="OHO85_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCQ2672952.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            3116..4615
                     /gene="pitA"
                     /locus_tag="OHO85_003632"
     CDS             3116..4615
                     /gene="pitA"
                     /locus_tag="OHO85_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCQ2672953.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(4686..5021)
                     /gene="uspB"
                     /locus_tag="OHO85_003633"
     CDS             complement(4686..5021)
                     /gene="uspB"
                     /locus_tag="OHO85_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCQ2672954.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            5412..5846
                     /gene="uspA"
                     /locus_tag="OHO85_003634"
     CDS             5412..5846
                     /gene="uspA"
                     /locus_tag="OHO85_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCQ2672955.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            6164..7633
                     /gene="dtpB"
                     /locus_tag="OHO85_003635"
     CDS             6164..7633
                     /gene="dtpB"
                     /locus_tag="OHO85_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCQ2672956.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(7682..8434)
                     /gene="rsmJ"
                     /locus_tag="OHO85_003636"
     CDS             complement(7682..8434)
                     /gene="rsmJ"
                     /locus_tag="OHO85_003636"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCQ2672957.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8442..10484)
                     /gene="prlC"
                     /locus_tag="OHO85_003637"
     CDS             complement(8442..10484)
                     /gene="prlC"
                     /locus_tag="OHO85_003637"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCQ2672958.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10687..11529
                     /gene="rlmJ"
                     /locus_tag="OHO85_003638"
     CDS             10687..11529
                     /gene="rlmJ"
                     /locus_tag="OHO85_003638"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCQ2672959.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            11601..12953
                     /gene="gorA"
                     /locus_tag="OHO85_003639"
     CDS             11601..12953
                     /gene="gorA"
                     /locus_tag="OHO85_003639"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCQ2672960.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(13007..13090)
                     /locus_tag="OHO85_003640"
     CDS             complement(13007..13090)
                     /locus_tag="OHO85_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCQ2672961.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            13830..14183
                     /gene="arsR"
                     /locus_tag="OHO85_003641"
     CDS             13830..14183
                     /gene="arsR"
                     /locus_tag="OHO85_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCQ2672962.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            14237..15526
                     /gene="arsB"
                     /locus_tag="OHO85_003642"
     CDS             14237..15526
                     /gene="arsB"
                     /locus_tag="OHO85_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCQ2672963.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLVALALRLSFTL"
     gene            15539..15964
                     /gene="arsC"
                     /locus_tag="OHO85_003643"
     CDS             15539..15964
                     /gene="arsC"
                     /locus_tag="OHO85_003643"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCQ2672964.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGITVRALLRKNVEPYEELSLAEDKFTDERLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
BASE COUNT         3731 a         4193 c         4103 g         4155 t
ORIGIN      
        1 atgatacaaa gcaataatac ttatatgttc gatgatttta agcaccgaga ctatccggtc
       61 ttttactaaa taatggaaaa caatccatcc ctggaagata attgtaaaaa aaacaacaga
      121 ataagaatta atcttaggat aaatatttat ttatcatggc cttttaaccg gtaaaaaatg
      181 gcactcggca cagtaaaccc cgctatagtt atgctatgca tttgtcttta atgatatctc
      241 gtcgttatat taacaggctg aaattatcat taatgcacat ttcattatta gcagggatac
      301 ctgaggagtt taaagtgaac atatatatcg ggtggctttt caaattaatc cctttgatta
      361 tgggattaat ttgcatcgcg ttaggtggct ttgtgctgga aagttcaggg caaagcgagt
      421 atttcgtcgc gggtcatgtg ctgatttctc tggcggccat atgcctggca ttattcacta
      481 ccgcatttat tatcatttcg cagctcacgc gcggcgttaa tacgttttac aatacattgt
      541 tccccattat tggctatgcg gggtcaatta tcaccatgat atggggttgg gcactgttag
      601 caggcaatga tgtgatggca gacgagtttg tcgccggcca tgttattttc ggcgttggta
      661 tgattgccgc ctgtgtatcg acggtggcag cgtcatccgg tcactttctg ctcattccca
      721 aaaatgcagc ggggagcaag agcgatggaa cgccggtaca ggcttattct tcattaatcg
      781 gtaattgcct cattgccgtt cccgttttac tcaccctgct cggtttcatt tggtctatta
      841 cgctgttacg tagtgctgac ataactccgc attatgtcgc gggtcacgta ttgcttgggt
      901 taaccgcaat ctgtgcctgt ctaattggcc ttgttgccac aattgtccat caaacacgta
      961 atacgttttc aactaaagaa cactggctgt ggtgttattg ggttattttt ctcggctcaa
     1021 tcacggtact gcaggggata tacgtcttag tcagttccga tgcaagcgcc cgactggctc
     1081 ccggcattat tcttatttgc ctcggaatga tctgttacag catattctca aaagtctggc
     1141 tactggcact ggtatggaga cgtacctgtt cgttagccaa cagaataccg atgattcccg
     1201 tcttcacctg cctgttttgc cttttcctgg catcgtttct tgcggaaatg gcgcagaccg
     1261 acatgggata ttttattcct tcgcgagttc tggtcggttt gggagcggta tgctttacgt
     1321 tgttctcaat cgtttcaata ttagaagcgg gttctgctaa aaaataattg caacgtaccg
     1381 gataaaacca gcgttgacca tttgcgtaac gctggttttt cttaggcatc atgaaataac
     1441 gcagcattaa tgcatagtgg ttaagtataa aaaacagcaa agttactgtt tttttcaacc
     1501 tgttcatatt tcataaagat ctggccactg ggtgagtttt caatcacctt tccatccacc
     1561 ttatattaag catggagggt ttcagttcac gggctcatta gaaaataatc acaagataac
     1621 cactatcaaa attagctcat ttttaatgcg actctaatag ttttcatctt taggaaatag
     1681 gtcaggacga ctttgctgca atcaaatcct gcgcacaagc ccacgcactc gaccacgccc
     1741 actggaagtt atagcccccc agccagccgg taacgtccat cacttcgccg atgaagtaca
     1801 gcccaggcac tttgcgcgct tccatcgtcc gtgaagagag ttcgttggtg tccacgccgc
     1861 cgagcgtcac ttcggcagtg cgatagcctt cagtgccgtt gggttgtacg cgccagtcgg
     1921 tcaatgtgct aatcagtgcc tgttggtcac gcacgttgag ctgttttagc gaaacatccg
     1981 ggatttgccc gagttgctgt aagcgttcaa ccaaccgctt cggtagatga accgccagtg
     2041 tgtttttcag gctctgattc ggatgtgcgt tacgctgctc attcaggaag gtttcgagat
     2101 ccacatccgg tagcagattg atactgacaa attcacccgg ttgccagtag ctggaaatct
     2161 gcaacaccgc cggtccagac aagccgcgat gggtgaagag taagttctca cggaaaacga
     2221 tgccgttttc agcggtaatc acggaaggca ccgccacgcc cgccagcacc tgtaactctt
     2281 cgagcaacgg tttatgcaga gtgaatggca ccagacccgc gcgggtcggc agcacgttga
     2341 ggccaaattg ttcggcaatc ttataaccaa acggcgacgc gcccagcccc ggcattgaca
     2401 gcccaccggt cgcgatgacc agcttttcgc aaccgacagt catgccgttc agttcaagcg
     2461 tgaagcctgt ttcatccttc gccacactca gcacttcgct acgcaatctg aaggttacat
     2521 tgcccttctc gcactcatcc accagcatgt cgacaatctg ctgcgcggag tcatcgcaga
     2581 agagttgtcc taacgttttc tcgtgccagg cgatgccgtg tttattgacc agatcaatga
     2641 aatcccactg ggtaaaacgc gcgagtgcag acttacaaaa atgcggattc tggctcagat
     2701 aagcgcctgg ttcgacataa aggttggtaa agttgcagcg cccaccgcca gacataagga
     2761 ttttgcgccc tggtttttta ccattatcga tcagcagaac ccggcgtcct gcctgacctg
     2821 ccagcgcaga acagaacata cccgccgcac cagcgcctat aataatggca tcaaaccttt
     2881 ccacgttgcg ctcctcttag aaaaaacggg cgtgaattgt aaagattcct cagtggtcgc
     2941 accagcatca atattactaa aaggaagtat ttgcctgaat tatataagat aattattttt
     3001 tgagtgaaat ccatacagga ggcaaatcaa aaaaagtcta tatttcactt tgcccgcgcc
     3061 gcgaaagtca ctgataatgc gccgcgttca tgtcctcaaa atggcgtaac gtcctatgct
     3121 acatttgttt gctggcctgg atttgcatac cgggctgtta ttattgcttg cactggcttt
     3181 tgtgctgttc tacgaagcca tcaatggttt ccatgacaca gccaacgccg tggcaaccgt
     3241 tatctatacc cgcgcgatgc gttctcagct cgccgtggtt atggcggcgg tgttcaactt
     3301 tttgggtgtt ttgctgggtg gtctgagtgt tgcctatgcc attgtgcata tgctgccgac
     3361 ggatctgctg cttaatatgg gatcgtctca tggccttgcc atggtgttct ctatgttgct
     3421 ggcggcgatt atctggaacc tgggtacctg gtactttggt ttacctgcat ccagctctca
     3481 tacgctgatt ggcgcgatca tcgggattgg tttaaccaat gcgttgatga ccgggacgtc
     3541 agtggtggat gcactcaata tcccgaaagt attaagtatt ttcggttctc tgatcgtttc
     3601 ccctattgtc ggcctggtgt ttgctggcgg tctgattttc ttgctgcgtc gctactggag
     3661 cggcaccaag aaacgcgccc gtatccacct gaccccagcg gagcgtgaaa agaaagacgg
     3721 caagaaaaag ccgccgttct ggacgcgtat cgcgctgatc ctttccgcta tcggcgtggc
     3781 gttttcgcac ggcgcgaacg atggtcagaa aggcattggt ctggttatgt tggtattgat
     3841 tggcgtcgcg ccagcaggct tcgtggtgaa catgaatgcc actggctacg aaatcacccg
     3901 tacccgtgat gccatcaaca acgtcgaagc ttactttgag cagcatcctg cgctgctcaa
     3961 acaggctacc ggtgctgatc agttagtacc ggctccggaa gctggcgcaa cgcaacctgc
     4021 ggagttccac tgccatccgt cgaataccat taacgcgctc aaccgcctga aaggtatgtt
     4081 gaccaccgat gtggaaagct acgacaagct gtcgcttgat caacgtagcc agatgcgccg
     4141 cattatgctg tgcgtttctg acactatcga caaagtggtg aagatgcctg gcgtgagtgc
     4201 tgacgatcag cgcctgttga agaaactgaa gtccgacatg cttagcacca tcgagtatgc
     4261 accggtgtgg atcatcatgg cggtcgcgct ggcgttaggt atcggtacga tgattggctg
     4321 gcgccgtgtg gcaacgacta tcggtgagaa aatcggtaag aaaggcatga cctacgctca
     4381 ggggatgtct gcccagatga cggcggcagt gtctatcggc ctggcgagtt ataccgggat
     4441 gccggtttcc actactcacg tactctcctc ttctgtcgcg gggacgatgg tggtagatgg
     4501 cggcggctta cagcgtaaaa ccgtaaccag tattctgatg gcctgggtgt ttacccttcc
     4561 ggctgcggta ctgctttccg gcgggctgta ctggctctcc ttgcagttcc tgtaatcgta
     4621 cgcaccaaaa cgagcgggtc agtttactgg cccgcttttt ttatgcgtta ttattgctga
     4681 agtgcttaat gccaaatcat caatgcaatc aggctgacca ccaccagacc acacaatgcg
     4741 ctagtcagaa taaactgccg acgcacccgc tcacagcggc gaataaactc atcatcgtga
     4801 tgatcgcgat aacgttgggc atagatatac caaacgagac gcacctgttt gttgggttgg
     4861 ccatgtgagg taaaaaagcc ccctccatca acatattgat agagcaatgg atcgcagtta
     4921 cgcagtacca ctaacaacgc gcgtagtgat gagaaatagc gcgccatgtt aacaatgcaa
     4981 acgacacata aagcccaaaa taatgcgacg gtgcttatca tacctcctcc ccggcgacct
     5041 gcccgcggag ttccaccccg gggctaccgc tcccgatacg ctgccaatca gttaacacca
     5101 ggtcctggag aaaccgcttt tgtggtgacc aacatccgag cggctctata gatagtgtag
     5161 gagatcaggt tgtttttttt ccagaaggtt aaccactatc aatatattca tgtcgaaaat
     5221 ttgtttatct aacgagtaag caaggcggat tgacggatca tccgggtcgc tataaggtaa
     5281 ggatggtctt aacactgaat ctttacggct gggttagccc cgcgcacgta gttcgcagga
     5341 cgcgggtgac gtaacggcac aagaaacgct agctggccag tcatcgacaa ctttatggaa
     5401 ggagtaacac tatggcttat aaacacattc tcatcgcggt cgacctctcc ccggaaagca
     5461 aagttctggt agagaaagca gtctctatgg ctcgccccta caatgcgaaa gtttctctga
     5521 tccacgtaga tgtaaactac tctgacctat acaccgggct tattgatgtg aatctgggtg
     5581 atatgcagaa acgcatctct gaagagacac atcatgcgct gaccgagctt tccactaatg
     5641 caggctaccc aatcactgaa accctgagcg gcagcggcga cctgggccag gttctggtcg
     5701 atgcaatcaa gaaatacgat atggatctgg tggtttgtgg tcaccaccag gacttctgga
     5761 gcaaactaat gtcttccgca cgtcagctga tcaacaccgt tcacgttgat atgctgattg
     5821 ttccgctgcg cgacgaagaa gaataatcat ccccccacga cgtgttcctg aacgcccgca
     5881 tatgcgggcg ttttgctttt tggcgcgcct tgttacctga tgcagcgtaa accccttatc
     5941 tggcctacga tctgcgtacg caatcaaaat ctcctgccaa tacaacattt aacaccatca
     6001 tattttccat cattagtgtg atcagctggt tattttctgt tgtaatagtg tattaatcta
     6061 ttcaccgcat caatattaag aatctctgac agatgtaaac tttttcgcgc gttatccctt
     6121 acgcgttcat acttttcagg atggtattgg aaggttaata aatatgaata caacaacacc
     6181 catggggatg ctgcagcaac ctcgcccatt tttcatgatc ttttttgtcg agttatggga
     6241 gcgattcggc tactacggcg tgcagggcgt actggcggtt ttcttcgtta aacagcttgg
     6301 attctcgcaa gagcaggctt ttgtcacttt tggtgctttt gctgcgctgg tctatggcct
     6361 catttccatt ggcggctatg tcggcgacca cctgctgggg accaaacgca ccattgttct
     6421 cggagcactt gtgctggcga ttggctactt catgaccggc atgtcgctac ttaagcctga
     6481 cctgattttc atcgccctgg ggactatcgc tgtcggtaac ggcctgttta aagctaaccc
     6541 agccagcttg ctttcgaagt gctatccgcc gaaagatccg cggcttgatg gcgcattcac
     6601 cctgttctat atgtcgatca atatcggctc gttgatagcg ttatcgctgg cccctgtgat
     6661 cgctgataga ttcggttatt cagtcaccta caacctgtgc ggggcggggt taattatcgc
     6721 attactggtt tacatcgcct gtcgtggaat ggtgaaagac attggttctg aacccgactt
     6781 caagccgatg agcttcagca aactgttgta cgtattactt ggcagcgtgg tgatgatctt
     6841 cgtatgtgca tggctgatgc acaacgtaga agtcgccaat ctggtgctga ttgttctctc
     6901 catcgtcgtc accatcatct tctttcgtca ggcattcaag ctggataaaa ccgggcgcaa
     6961 taaaatgttt gtcgcctttg tcctgatgct cgaagcggtg gtgttttaca ttctctacgc
     7021 ccagatgcca acatcgctga acttctttgc catcaacaac gtgcatcatg aaattctcgg
     7081 tttttccatc aacccggtca gcttccaggc gcttaacccg ttctgggtgg tactcgccag
     7141 cccaatactg gcaggcattt acacgcatct gggtaacaaa ggcaaagacc tctcgatgcc
     7201 gatgaaattt actctcggca tgtttatgtg ctctctgggc tttttgacgg ctgcagctgc
     7261 gggaatgtgg tttgcggatg cccaggggct gacatcgcca tggtttatcg tgctggtgta
     7321 cttattccag agcttaggtg aactgtttat tagcgccctt ggcctggcga tgattgctgc
     7381 cctggtgccg cagcatttga tgggctttat tctcgggatg tggttcctga cgcaggctgc
     7441 cgcgttcttg ctgggcggct atgtggcaac atttaccgcg gtgccggaca acattaccga
     7501 tccgcttgag acgttgcccg tctataccaa cgtgtttggt aagattggtc tggtcacact
     7561 gggcgttgca gtagtgatgc tgttgatggt gccgtggctg aaacgcatga ttgcgacgcc
     7621 ggaaagccat taattattct tgcagaaagc agggtagcgt tatcgctacc ctgctttact
     7681 tttacacggg cgtacctgca tagatatcaa agcgatgccc tttagtcaca accgcgtttg
     7741 gcgtggcgac attcgctagc ggtggcgcgt agtccgggcg ctttactacc acgcgtttgg
     7801 tcgccagcaa gcgtgcaggc tccagcaatc catcggcatc aagatccggc cccaccagcg
     7861 actgaaagac acgcatctct tttttcacca gcgcgctttt ctgcttatgc gggaacatcg
     7921 ggtcgagata gaccacctgc gggcgtgggg taatatcggt tagcgccgtc aggctggagg
     7981 cgtgaattaa ctgcaaacgc tcctgcaacc agccgccgat ttccgcatcc gcataaccac
     8041 gcgccaggcc gtcgtcgagc aatgcggcaa cgactggatt acgctccagc atccgcacgc
     8101 ggcaaccgac tgaagccagc acaaaggcat cgcgccccag ccctgcggtg gcgtccacca
     8161 catccggcaa ataatcgcct ttaatgccca ccgctttcgc caccgcctca ccgcgaccac
     8221 cgccgaattt gcgtcggtgc gccatcgctc cgccaacaaa atcaacaaat atgccgccaa
     8281 gttttggctc atcacgcttg cgcaattcca gatgttccgg cgttaacacc agcgccatca
     8341 ggttgtcttc atcgtgctcc agcccccagc gggccgccag aacagataag gcgccgtctc
     8401 cggcgcctgt ttcatcaatt aagcagattt tcactgaatg atcagccctt aatgccgtaa
     8461 tgctccagca tcgcatccag ctgcggttca cgaccacgga agcgtttgaa cagatccatc
     8521 ggctcttctg aaccgccacg gctcagaatg ttgtcgagga acgactgccc ggtttcacgg
     8581 ttgaaaatgc cctcttcctc aaagcgcgag aaagcatctg ccgccagtac gtcagcccac
     8641 aggtagctgt agtaacctgc cgcataacca ccggcgaaaa tatggctgaa agcgtgcggg
     8701 aaacggcccc aggacggaga tggcaccacg gcaaccagtt tcttgatttc tgccagagtt
     8761 tcgaggattt ttgccccctg atccgggcgg aactcagcat gaaggcggaa atcaaacagg
     8821 ccgaactcca gctgacgcag aataaacagc gccgcctggt agttcttcgc cgccagcatt
     8881 ttatccagca actctttcgg cagcggttcg ccggtttcat agtgaccgga gataaacgcc
     8941 agcgcctccg gctcccagca ccagttttcc ataaactgac tcggcagttc gaccgcatcc
     9001 cacggcacac cgctgatacc ggaaacacca gcggtttcga tgcgggtcag catatggtgc
     9061 aggccgtgac cgaactcgtg gaacagggtg atcacttcgt cgtgagtgaa cagcgccggt
     9121 ttaccattta ccgggcggtt gaagttacaa gtcaaatacg cgaccggttt ttgcagcgaa
     9181 ccatcagctt tacgcatctg gcctacgcag tcatccatcc acgccccgcc gcgcttgttt
     9241 tcacgggcat acagatcgag gtagaagctg ccgcgcagtt cgttattttc gtcatacagt
     9301 tcgaagaaac gtacatccgg atgccagaca tcaacatctt tacgctcttt agcggtgatg
     9361 ccgtaaatac gtttcaccac ttcaaacagg ccgttaaccg ctttgttttc cgggaagtac
     9421 ggacgcagct gttcgtcact gatgctgtag aggtgctgtt tttgtttttc gctgtagtac
     9481 gcgatatccc acggctgcaa ctcatcgacg ccaaattccg ctttggcgaa ggcgcgcagt
     9541 tgtgccagct ctttttcgcc ttgcggacgc gcgcgttttg ccagatcggt taagaaatcc
     9601 agcacctgct gcgggttttc tgccatttta gtagcaaggg atttaaaggc gtagttttca
     9661 aagcccagca gttgcgccag ttcgtgacgc agagcgagga tctcttccat caccttgctg
     9721 ttatcccact taccggcgtt cgggccttga tcggaagcgc gggtgctgta agcgcgatac
     9781 atctcttcac gcaaggcctg gttgtcgcag taggtcatta ccggcaggta gcttgggata
     9841 tccagcgtca gcaggtagcc ttccagctct ttcgcttcgg cctgggcttt tgccgcagcc
     9901 agcgcgcttt caggcatccc cgccagctcc gcttcgtcgg taacgagttt ggtccagccc
     9961 atcgtcgcat cgaggacgtt gttgctgtac tggttgccca gctcggaaag acgagtggca
    10021 atctcaccgt agcgttgttg tttctctatt ggcagaccga tgccagagag ttcgaagtcg
    10081 cgcagtgcgt tatcaaccgc ttttttctga gccgtgttca gcgtggcgta atgatcgcca
    10141 tcgcgcaggt cgcgatacgc tttatacagc ccttcatgtt gccctaccca ggtgctgtat
    10201 tccgacagca gcggcagggt ttgttcgtac gcttcacgca gttccgggct atttttcacc
    10261 gagttcaggt ggctgaccgg ggagaagata cgccccagca catcgtccac ttccgccaac
    10321 ggctggcaga gattttccca ggtgtacggt gctccttgcg ctactacgcg ctccacattt
    10381 tcgcggcagt cgttcagcgc cttagtcacg gctggaacga catgttccgg gagaatttta
    10441 gaaaacggag gcaattcaaa gggagtcagt aacggattcg tcattcgcgc agtcctggtt
    10501 aaagaggtta aggaagcgct caacaggcgc tttacataat gtgtgtagca tggggttaag
    10561 tgtagtgaat ttcaatgaga aacgttacgc tttcgcggcg gcggcctctt ttcggtatac
    10621 tgtcctgata agcttttgtg cgccccgaat acgggccgat ttttacttac cggaacacct
    10681 ttacccatgc tcagttatcg ccacagcttt cacgctggca accacgccga cgtccttaaa
    10741 cataccgttc agagcctgat catcgagtcg ctgaaagaga aagataaacc gtttctctat
    10801 ctcgacaccc acgcaggggc cgggcgttat cagttaggca gcgaacatgc cgagcgtacc
    10861 ggcgaatatc tcgaaggcat cgcccgtatc tggcagcagg acgatttgcc cgcagaactg
    10921 gaggcgtaca tcaatgtggt aaaacacttc aaccgtagcg gtcagttgcg ttactacccc
    10981 ggttcgccat tgattgctcg ccagctactg cgcgaacaag acagcctgca actgaccgaa
    11041 ctgcacccga gcgattaccc gttgctgcgt tctgaatttc agaaagacag ccgtgcgcgc
    11101 gtcgaaaaag ccgatggttt ccagcagctt aaggccaaac tgccgccggt ttcccgccgt
    11161 ggtttaatcc ttatcgaccc gccgtatgaa atgaaaaccg actatcaggc ggtggtcagc
    11221 gggatagcag aaggttacaa acgtttcgcc actggtactt acgcactgtg gtatccggtg
    11281 gtgctgcgtc agcaaattaa gcgcatgatc cacgatctgg aagcgaccgg tattcgcaaa
    11341 attctgcaaa ttgaactggc ggtactgcca gacagcgatc gccgtggcat gaccgcttcc
    11401 ggcatgattg tgattaaccc gccgtggaaa ctggaacagc agatgaataa cgtgctgccg
    11461 tggctgcaca gcaaactggt tccggcaggc accgggcacg ccaccgtaag ctggatcgtg
    11521 ccggagtaat tgcagccatt gctggcacct attacgtctc gcgctacaat cgcggtaatc
    11581 aacgataagg acactttgtc atgactaaac actatgatta catcgccatc ggcggcggca
    11641 gcggcggtat cgcctccatc aaccgcgcgg ctatgtacgg ccagaaatgt gcgctgattg
    11701 aagccaaaga gctgggcggc acctgcgtaa atgttggctg tgtgccgaaa aaagtgatgt
    11761 ggcacgcggc gcaaatccgt gaagcgatcc atatgtacgg cccggattat ggttttgata
    11821 ccactatcaa taaattcaac tgggaaacgt tgatcgccag ccgtaccgcc tatatcgacc
    11881 gtattcatac ttcctatgaa aacgtgctcg gtaaaaataa cgttgatgta atcaaaggct
    11941 ttgcccgctt cgttgatgcc aaaacgctgg aggtaaacgg cgaaaccatc acggccgatc
    12001 atattctgat cgccacaggc ggtcgtccga gccacccgga tattccgggc gtggaatacg
    12061 gtattgattc tgatggcttc ttcgcccttc ctgctttgcc agagcgcgtg gcggttgttg
    12121 gcgcgggtta catcgccgtt gagctggcgg gcgtgattaa cggcctcggc gcgaaaacgc
    12181 atctgtttgt gcgtaaacat gcgccgctgc gcagcttcga cccgatgatt tccgaaacgc
    12241 tggtcgaagt gatgaacgcc gaaggcccgc agctgcacac caacgccatc ccgaaagcgg
    12301 tagtgaaaaa tgccgatggt agcctgacgc tggagctgga agatggtcgc agtgaaacgg
    12361 tggattgcct gatttgggcg attggtcgcg agcctgccaa tgacaacatc aacctggaag
    12421 ccgctggcgt taaaactaac gaaaaaggct atatcgtcgt cgataaatat caaaacacca
    12481 atattgaagg tatttacgcg gtgggcgata acacgggtgc agtggagctg acaccggtgg
    12541 cagttgcagc gggtcgccgt ctctctgaac gcctgtttaa taacaagccg gatgagcatc
    12601 tggattacag caacattccg accgtggtct tcagccatcc gccgattggt actgttggtt
    12661 taacggaacc gcaggcgcgc gagcagtatg gcgacgatca ggtgaaagtg tataaatcct
    12721 ctttcaccgc gatgtatacc gccgtcacca ctcaccgcca gccgtgccgc atgaagctgg
    12781 tgtgcgttgg atcggaagag aagattgtcg gtattcacgg cattggcttt ggtatggacg
    12841 aaatgttgca gggcttcgcg gtggcgctga agatgggtgc aaccaaaaaa gacttcgaca
    12901 ataccgtcgc cattcaccca acggcggcag aagagttcgt gacaatgcgt taaatgttaa
    12961 agggctaaga gtagtgtgct cttagccctt aattacgttt ccgctatcag ttcagaagct
    13021 gaagcagaaa gcggatcagt tccagcagcg caattaacgc cccaagaacg atgattgctt
    13081 tatcaatcac ccgttttctc catgcgatgg agtgagaatg catccgctta ctcatccact
    13141 gcctgtcacg gcgcatgccc tattgttaga taatggcatt ctcactccgg ccagagcatc
    13201 agttaacggc accacccgta cttctgacca ggactttgaa agcgtttatg cgcattacca
    13261 gagtgaaaat gcctcagagc taactggata atcatacagt acattcaggt tataaaacca
    13321 gcatgtcctt gcaatagttt cagtatggta ttagcattga tgcgttagat aatggcattc
    13381 tcactccagc cagtgccacc aactcagggc tggaaagtaa aaaaccgacg caaagtcggt
    13441 ttttttacat ccggattcgg acaaggctta atatgacgat gacccagtga aagtatataa
    13501 atcgtcattg cgatatatac cgaagtgccc cctccgccag ctgaagaaat cgctaattct
    13561 tgcaatgtta gccactggct aatagtattg agctgttaga taagaactct ctcactccag
    13621 ccagagccac caactcaggg ctggaaagta aaaaaccgac gcaaagtcgg tttttttacg
    13681 tcctgattca gacctccttt caaatgaata gccaactcaa aattcacacc tattaccttc
    13741 ctctgcactt acacattcgt taagtcatat atgtttttga cttatccgct tcgaagagag
    13801 acactacctg caacaatcag gagcgcaata tgtcatttct gttacccatc caattgttca
    13861 aaattcttgc tgatgaaacc cgtctgggca tcgttttact gctcagcgaa ctgggagagt
    13921 tatgcgtctg cgatctctgc actgctctcg accagtcgca gcccaagatc tcccgccacc
    13981 tggcattgct gcgtgaaagc gggctattgc tggaccgcaa gcaaggtaag tgggttcatt
    14041 accgcttatc accgcatatt ccagcatggg cggcgaaaat tattgatgag gcctggcgat
    14101 gtgaacagga aaaggttcag gcgattgtcc gcaacctggc tcgacaaaac tgttccgggg
    14161 acagtaagaa catttgcagt taaaaattta gctaaacaca tatgaatttt cagatgtgtt
    14221 ttatccggga ggcattatgt tactggcagg cgctatcttt gtcctgacca tcgtattggt
    14281 tatctggcag ccgaaaggtt taggcatcgg ctggagtgca acgctcggcg cagtactggc
    14341 gttagttacg ggcgtggtcc atccgggtga tattccggtg gtgtggaata tcgtctggaa
    14401 cgcgacggct gcgtttatcg ccgtcattat catcagcctg ctgctggatg agtccggctt
    14461 ttttgaatgg gcggcgctgc acgtctcacg ctggggtaat ggtcgtggtc gcttgctgtt
    14521 tacctggatt gtcctgctcg gtgctgccgt tgccgccctg tttgccaatg atggcgcggc
    14581 gcttattttg acaccgattg tcatcgccat gctgctggct ttagggttca gtaaaggcac
    14641 tacgctggcg ttcgtgatgg cggccggatt cattgccgat accgccagcc tgccgcttat
    14701 tgtctccaac ctggtgaata tcgtttccgc tgatttcttt ggcctcggct ttcgcgaata
    14761 cgcctcggtg atggtgccgg tggatatcgc cgcgattgtt gccacgctgg tgatgttaca
    14821 tctctatttt cgcaaagata ttccgcagaa ctacgatatg gcgctgctga aatctcccgc
    14881 agaagcgatc aaagatcctg ctacgttcaa aactggctgg gttgttttac tgcttctgct
    14941 ggtgggattt ttcgtcctgg aaccgctcgg cattccggtg agcgccattg cagctgtggg
    15001 cgcgctgata ttatttgtcg tcgctaaacg cggtcatgcg attaatacgg gtaaagtcct
    15061 gcgcggtgcc ccctggcaga ttgtcatctt ctcgctcggc atgtatctgg tggtttatgg
    15121 cctgcgcaat gccggattaa cggaatatct ttctggcgta ctcaacgtgc tggcggataa
    15181 cggcctgtgg gccgcgacgc tcggcaccgg attcctcacc gccttcctct cttctattat
    15241 gaacaatatg ccgacggtac tggttggcgc gttgtccatt gatggcagca cggcatctgg
    15301 cgttatcaaa gaagcgatgg tttatgccaa tgtgattggc tgcgatttgg gaccgaaaat
    15361 taccccgatt ggtagcctgg caacgctgct ctggctgcac gtactttcgc agaagaatat
    15421 gactatcagc tggggatatt acttccgtac agggattatc atgaccctgc ctgtgctgtt
    15481 tgtgacactc gtcgcgctgg cgcttcgtct ctctttcact ttgtaatgag atactgatat
    15541 gagcaacatt accatttatc acaacccggc ctgcggcaca tcgcgtaata cgctggagat
    15601 gatccgcaac agcggtacag aaccgaccat tatccattat ctggaaacac caccaacgcg
    15661 cgatgaactg gtaaaactca ttgccgatat ggggattacc gtacgcgcgc tgctgcgtaa
    15721 aaacgtcgag ccgtatgagg aactgagcct tgcggaagat aaatttactg acgagcggtt
    15781 aatcgacttt atgcttcagc acccgattct gattaaccgt ccgattgtgg tgacgccgct
    15841 gggaactcgc ctatgccgcc cctcggaagt ggtgctggaa attctgccag atgcgcaaaa
    15901 aggcgcgttc tccaaggaag atggcgagaa agtggttgat gaagcgggta agcggttgaa
    15961 ataaagcggc gatatccact gccgatagtt ggtaaagtgc gctttgttta tgtcggatgc
    16021 gacgctaacg cgtcttatcc gatctacaaa gtcatgaaaa ttcaataaat tgtatgttat
    16081 ttgtagacct gacaggcgta gcgcatcagg cgattttgct atttacacat tactccctgc
    16141 gtgagattcc aattatcgcg tccagcatgg tgtatcagtg at
//
LOCUS       DAJOWB010000106        15924 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJOWB010000106 DAJOWB010000000
VERSION     DAJOWB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.106"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            53..478
                     /locus_tag="OHO85_003644"
     CDS             53..478
                     /locus_tag="OHO85_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCQ2672965.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            475..825
                     /gene="tnpB"
                     /locus_tag="OHO85_003645"
     CDS             475..825
                     /gene="tnpB"
                     /locus_tag="OHO85_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCQ2672966.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            856..2469
                     /locus_tag="OHO85_003646"
     CDS             856..2469
                     /locus_tag="OHO85_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HCQ2672967.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKMRRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDACCQLMTPVNDALYRYVMNTRKVHTDDTPVKVLA
                     PGQKKAKTGRIWTYVRDDRNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAF
                     TGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            2511..3152
                     /locus_tag="OHO85_003647"
     CDS             2511..3152
                     /locus_tag="OHO85_003647"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCQ2672968.1"
                     /translation="MERIKLAEKIISTCREMNASGLNQGTSGNVSARYTGGMLITPSG
                     IAYSKMTPDMIVFVDDKGKPEAGKIPSSEWLIHLACYKARPELNAVIHTHAVNSTAVA
                     IHNHSIPAIHYMVAVSGTDHIPCIPYYTFGSPELADGVSKGIRESKSLLMQHHGMLAM
                     DVTLEKTLWLAGETETLADLYIKCGGLHHDVPVLSEAEMTIVLEKFKTYGLKA"
     gene            3256..4260
                     /locus_tag="OHO85_003648"
     CDS             3256..4260
                     /locus_tag="OHO85_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HCQ2672969.1"
                     /translation="MFIVESYAVAIIMCFITMICWGSWANTTKLVSNKKWEFPLFYWD
                     YSIGLLLCSLLFAFTLGSMGEAGRSFIPDIQQASSSSLMSAILAGIIFNISNILLVAS
                     INLAGMAVAFPVGVGLALALGVITTYIGNPQGDPLILFLGVVCVVIAIIFTAIAYGRV
                     TQEADKSRRNKGLITAILAGIIMGWFFRFLADSMSDNFSQPASGLMTPYSALVLFAVG
                     LFLSNFVLNRLVMKKPISGEPVNGKMYFSGSLRDHVCGWLGGMIWCVGLAFSLIASGQ
                     AGYAISYGLGQGATMIAVIWGVFIWREFASAPAGTNKLLLTMFISYIVGIVLIIAANQ
                     "
     gene            complement(4301..5287)
                     /locus_tag="OHO85_003649"
     CDS             complement(4301..5287)
                     /locus_tag="OHO85_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCQ2672970.1"
                     /translation="MDRDQTNSSLFNDDPVLHATWLYYQEGKSQTEVAAIMGVSRVTV
                     VKYLQTARENGLVHINLDVNVFGSIDAALQIRDKFNLQRVIIVPDGEHAGKRDDTKLM
                     RTRLSRAGGMYLNQVIENGDVLGVAWGRTIHQMSKTMTPKSCKNVTVIQMLGSMPSQP
                     DLTIIESSSQIAYKLSGRVASLHVPAVVSSARLAMELQAEPIIRSNFDVLTRCTKAFF
                     VVGNALDENPLIRVGVLNKKEMQTYRDLGAVGVICGRFYDKEGMPVVADVDQRILGIS
                     LAQLRQIERKIFLAGGERGYDATLGALLGGYVTDLIVDEGTAEFLLACELPH"
     gene            complement(5634..6983)
                     /locus_tag="OHO85_003650"
     CDS             complement(5634..6983)
                     /locus_tag="OHO85_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HCQ2672971.1"
                     /translation="MPLIIVVAGIALLLLLTIKIKLNTFVSLIIVSIAVAIASGMDLS
                     KVVTSVESGLGGTLGHIGLIFGFGVMLGRLLADAGGAQRIALTMLNYFGKNKLDWAVV
                     CSAFIVGIALFFEVGLILLVPILFAIAREAKISPMFMCVPMLSGLLVAHGFLPPHPGP
                     TVIAREYGADVGLVLIYGIIVGIPTFILCGPVLNKFCQRIIPDAFKKEGNIASLGATR
                     RFSESEMPGFGISFLTAMLPVILMAVVTIIQMTHAKSAADSGLFYNVILFLGNSTIAM
                     LISLLFAIYTMGLGRGKTIPDLMDSCGKAIAGIAGLLLIIGGGGAFKQVLIDSGVGQY
                     ISTLVSGMDINPILMAWGVAAFLRICLGSATVAAISTAGLVIPLLAVHPNTNLALITL
                     ATGAGSCICSHVNDASFWMIKDFFGLTTKETLLSWTLMSTLLSISGLIFILLASLVL"
     gene            complement(7090..9057)
                     /gene="yjhG"
                     /locus_tag="OHO85_003651"
     CDS             complement(7090..9057)
                     /gene="yjhG"
                     /locus_tag="OHO85_003651"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylonate dehydratase YjhG"
                     /protein_id="HCQ2672972.1"
                     /translation="MSVRNIFADESHDIYTVRTHADGPDGELPLTAEMLINRPSGDLF
                     GMTMNAGMGWSPDELDRDGILLLSTLGGLRGADGKPVALALHQGHYELDIQMKAAAEV
                     IKANHALPYAVYVSDPCDGRTQGTTGMFDSLPYRNDASMVMRRLIRSLPDAKAVIGVA
                     SCDKGLPATMMALAAQHNIATVLVPGGATLPAKDGEDNGKVQTIGARFANGELSLQDA
                     RRAGCKACASSGGGCQFLGTAGTSQVVAEGLGLAIPHSALAPSGEPVWREIARASARA
                     ALNLSQKGITTREILTDKAIENAMTVHAAFGGSTNLLLHIPAIAHQAGCHIPTVDDWI
                     RINKRVPRLVSVLPNGPVYHPTVNAFMAGGVPEVMLHLRSLGLLHEDVMTVTGSTLKE
                     NLDWWEHSERRQRFKQLLLDQEQINADEVIMSPQQAKARGLTSTITFPVGNIAPEGSV
                     IKSTAIDPSMIDEQGIYYHKGVAKVYLSEKSAIYDIKHDKIKAGDILVIIGVGPSGTG
                     MEETYQVTSALKHLSYGKHVSLITDARFSGVSTGACIGHVGPEALAGGPIGKLRTGDL
                     IEIKIDCRELHGEVNFLGTRSDEQLPSQEEATAILNARPSHQDLLPDPELPDDTRLWA
                     MLQAVSGGTWTGCIYDVNKIGAALRDFMNKN"
     gene            complement(9068..9973)
                     /locus_tag="OHO85_003652"
     CDS             complement(9068..9973)
                     /locus_tag="OHO85_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HCQ2672973.1"
                     /translation="MKKFSGIIPPVSSTFHRDGTLDKKAMREVADFLINKGVDGLFYL
                     GTGGEFSQMNTAQRMALAEEAVTIVDGRVPVLIGVGSPSTDEAVKLAQHAQAYGADGI
                     VAINPYYWKVAPRNLDDYYQQIARSVTLPVILYNFPDLTGQDLTPETVTRLALQNENI
                     VGIKDTIDSVGHLRTMINTVKSVRPSFSVFCGYDDHLLNTMLLGGDGAITASANFAPE
                     LSVGIYRAWREGDLATAATLNKKLLQLPAIYALETPFVSLIKYSMQCVGLPVETYCLP
                     PILEASEEAKDKVHVLLTAQGILPV"
     gene            complement(9978..10766)
                     /locus_tag="OHO85_003653"
     CDS             complement(9978..10766)
                     /locus_tag="OHO85_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCQ2672974.1"
                     /translation="MVRKGCNSLVRAEKILTHIAWVGMASYMELLNKFQYPKSSLLNL
                     LNVMVDCGFLIKNKNGYYSLGIKNYELGCQALHRQNIFEVTKRPMQELSLKSGLVCHL
                     GAMESISAIYLDKIESPDSVPTSKSWIGKKLELHITALGKALLAWKTREELDYFLEAL
                     TLTPHTRNTFTDKKLFLEELQKTRLRGWAIDNEESTYGAVCLSMPVFNMYNRVNYAIS
                     LSGDPVVYSGNKIDSYLELLRKCAEQISYGLGYRNENEHLRKGN"
     gene            complement(11069..11851)
                     /locus_tag="OHO85_003654"
     CDS             complement(11069..11851)
                     /locus_tag="OHO85_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCQ2672975.1"
                     /translation="MSQQRPDRIKQMLHYLWQHRHLSTQQAMELFGYAEATVRRDFQY
                     IVNQYPGMIRGHGCLDFDDSTDDKEYVFDVKRTLQSVAKREIAALARTMIKDGDCFFL
                     DSGSTCLELAKCLADARVKVICNDIKIANELGCFPHVESYIIGGLIRPGYFSVGESLA
                     LEMINAFSVERAFISCDALSLETGITNATMFEVGVKTRIIQRSREVILMADHSKFDAV
                     EPHAVATLSCIKTIISDSGLPETIAQRYQRAGCQLFLSHSIK"
     gene            complement(11868..12500)
                     /locus_tag="OHO85_003655"
     CDS             complement(11868..12500)
                     /locus_tag="OHO85_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3 epimerase family protein"
                     /protein_id="HCQ2672976.1"
                     /translation="MILHPSLASANPLHYGRELTALDNLDFGSLHLDIEDSSFINNIT
                     FGMKTVQAVARQTPHPLSFHFMLARPQRWFNALAEIRPAWIFVHAETLDYPSETLTEI
                     RHTGARAGLVFNPATPIDAWRYLASELDGVMVMTSEPDGQGQRFIPSMCEKIQKVRTA
                     FPQTECWADGGITLAAAQQLAAAGAQHMVIGRALFSSSDYRATLAQFATL"
     gene            complement(12512..12943)
                     /locus_tag="OHO85_003656"
     CDS             complement(12512..12943)
                     /locus_tag="OHO85_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCQ2672977.1"
                     /translation="MINDIKWVQAQRKATDWRQAVEIATRPLVAYGAAQPCYVNGIIE
                     NTLNWGPYYLIAPGIALPHARPEQGANYNQVSITTLRTPVAFGNEECDPVWLLLCVSA
                     TDANAHILTIQRISQFIDSPQRLTAVGNASTDDALFALVSG"
     gene            complement(13074..13880)
                     /gene="sgcQ"
                     /locus_tag="OHO85_003657"
     CDS             complement(13074..13880)
                     /gene="sgcQ"
                     /locus_tag="OHO85_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA family protein SgcQ"
                     /protein_id="HCQ2672978.1"
                     /translation="MSWLKEVIGTEKAVIAMCHLRALPGDPSFDAQLGMNWVIDKAWD
                     DLMALQNGGVDAVMFSNEFSLPYLTKVRPETTAAMARIIGQLMSDIRIPFGVNVLWDP
                     VASFDLAMATGAKFIREIFTGAYASDFGVWDTNVGETIRHQHRIGAGEVKTLFNIVPE
                     AAVYLGNRDICSIAKSTVFNNHPDALCVSGLTAGTRTDSALLKRVKETVPDTVVLANT
                     GVCLENVEEQLSIADGCVTATTFKKDGVFANFVDQARVSQFMEKVHHIRR"
     gene            complement(13893..15206)
                     /locus_tag="OHO85_003658"
     CDS             complement(13893..15206)
                     /locus_tag="OHO85_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCQ2672979.1"
                     /translation="MFDYILSLGGTVFVPIIMIVIGLIFRIPWLQAIKAGVTVGIGFV
                     GMGLVIVMAIDSLSPPIKVMIERFGLALHVFDVGAGPASGVGYATAIGAMIIPVIFLL
                     NVAMLVTRLTKTMNVDIYNYWHYAITGTVVQLMTGSLIYGVLGAICHAALSLKMADWT
                     AKRVQNIVGLEGISIPQGYGSSSVPLFVLLDAIYEKIPFMKGRNIDAQEIQKRYGMVG
                     DPVIIGVVLGLIFGLAAGEGFKGCASLMITVAAIMVLFPRMIRLIVEGLLPISDGARK
                     FFQKYFKGREVYIGLDTAVTLGHPTTIAVGLLLIPIMLILASILPGNKVLPLADLPVA
                     PFFICMATVIHRGDLVRTLISGVIVMITVLLIATQFAPYFTEMALKGGFSFAGESAQI
                     SALSVGNMFGWSISELMSLGIIGVVVAVGIVASVVLFLRKRELSE"
     gene            complement(15218..15496)
                     /locus_tag="OHO85_003659"
     CDS             complement(15218..15496)
                     /locus_tag="OHO85_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCQ2672980.1"
                     /translation="MKKILVACGTGMSTSTMIAHKLQEFLTEQGISATTAQCCLNEIP
                     LNCNGMDLIVTSMRTNSDYGIPTLNGAALLTGINDDALKQQIKALLTQ"
     gene            complement(15493..15921)
                     /locus_tag="OHO85_003660"
     CDS             complement(15493..15921)
                     /locus_tag="OHO85_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672981.1"
                     /translation="MGIDITPSCDTPDLHDYSEVRINQGVGITCLNYHGRGTLAGLIT
                     PPRLIRMLEQTALEHNIPVQREVAPGVITETGYIQVEQDGIPCASLSIPCRYTHSPAE
                     VASLRDLTDCIRLLTALAGMSAAHFPVEPDSGTTQEAHPL"
BASE COUNT         3987 a         4034 c         4023 g         3880 t
ORIGIN      
        1 agacgagaat tggtaactta gacgcccatc tgatatagac ggacatctaa gtatggaatt
       61 acaggactgg cgaaaagaac ctcgtaaaaa ctattcgaat gaattcaaac ttcgtatggt
      121 ggaactggca tcacaacctg gagcttgtgt tgcacagatt gcacgtgaaa atggcgtcaa
      181 tgataatgtt attttcaaat ggctcaggct ctggcagaac gaagggcgtg tttcgcggcg
      241 tcttccggta acgacctctt ctgacactgg cgttgaatta ttacctgtag aaataacgcc
      301 ggatgagcag aaagaacctg tggcggccat tgcgccgtct ttatccactt ccactcagac
      361 cagagtcagt gccagttcct gcaaggtgga attccgtcac ggtaacatga cgctggaaaa
      421 tccatcgcca gagctgctca cagtgttgat ccgtgaactg accgggaggg gaagatgatc
      481 tcactcccat caggtacccg tatctggctc gttgccggcg ttaccgatat gcgtaaatcc
      541 ttcaacggac tgggagaaca ggtacaacat gtgctgaatg ataatccctt ctccggtcac
      601 ctgtttatct tccgtggccg acggggtgac accgtcaaaa ttctttgggc tgatgctgat
      661 ggtctgtgcc tgttcaccaa acgcctggag gaaggccagt ttatctggcc tgcggtacgt
      721 gacggcaagg tatccattac ccgctcgcaa ctggcaatgc tcctcgataa gctggactgg
      781 cgtcagccaa aaacatccag ccgtaactca ctgacaatgt tgtaaaaaac tcctgaccgc
      841 attataaaaa cggtcatgag tcagaaatac ctcattcgca tcgcagagct ggaaaggttg
      901 ctctctgagc aggctgaagc cctccgtcag aaagaccagc aactgagtct ggttgaagag
      961 acggaagcct tcctgcgctc tgcactgaca cgtgccgaag aaaagatcga agaagatgaa
     1021 cgggaaatag aacatctgcg ggctcagata gaaaaaatgc gccggatgct gttcggtacc
     1081 cgttctgaaa aactgcgtcg tgaagttgaa ctggctgagg ctctgctgaa acaacgtgaa
     1141 caggacagcg atcgttacag tgggcgggaa gacgatcctc aggttccccg ccagttgcga
     1201 cagtcgcgcc atcgtcgtcc gttaccggca caccttcccc gtgaaataca ccgcctggag
     1261 ccagaagaaa gctgttgccc ggagtgtggc ggtgagctgg attatctggg ggaagtcagc
     1321 gctgaacagc tggaactggt gagcagtgcc ctgaaagtga tccgcacaga acgggtaaaa
     1381 aaagcctgta caaaatgtga ctgtattgtt gaagcaccgg cgccgtcccg cccgatagag
     1441 cgtggtatcg cgggccccgg attacttgcc cgcgtgttaa cgggaaaata ctgcgaacat
     1501 ctgccactgt atcgtcagag tgaaatcttt gcccgccagg gtgtcgaact gagccgggcc
     1561 ttactctcca actgggttga cgcgtgctgc cagttaatga caccggtgaa tgatgccctg
     1621 taccgttatg taatgaacac ccgcaaggtt cacactgatg acacaccggt aaaggtactg
     1681 gcaccgggtc agaaaaaggc gaaaacaggg cgtatctgga cgtatgtccg ggatgatcgc
     1741 aatgtgggtt cgtcatctcc tccagcggtc tggttcgcgt actcgccgaa ccggcagggg
     1801 aaacacccgg agcaacacct ccgccccttc cggggtatcc tgcaggcgga tgcgttcaca
     1861 ggttacgaca ggttgttcag tgcagaacgt gaaggtggtg cactgacaga agttgcgtgc
     1921 tgggcccatg cccggcgaaa aatccacgat gtatacatca gcagcaaaag tgcgacggca
     1981 gaagaagccc tgaagcgaat cagtgaactg tacgccatcg aggatgaaat acggggatta
     2041 ccggagtcag agcgtcttgc cgtcaggcag cagcgaagca aagtgttact gacgtcgctg
     2101 catgaatgga tggtggagaa gaatggtacg ctgtcgaaaa aatccagact gggcgaagcg
     2161 ttcagctatg tactgaatca gtgggatgcc ctctgttatt acagtgatga cggtctggcg
     2221 gaggcggata ataatgctgc ggaaagagcg cttcgtgcag tctgtctcgg aaagaaaaac
     2281 tttatgttct ttggcagcga tcacggcggc gagcgtggag cactgttgta cgggctgatc
     2341 ggcacctgcc gtctgaacgg tatcgatccg gaagcgtatc tgcgccatat cctgagcgta
     2401 ctgccggaat ggccttccaa ccgagttgac gaactcctgc catggaacgt agtactcacc
     2461 aataaataag cgtcaatacg gtgctccgtt gacgcttaca aggatcagga atggaacgga
     2521 ttaagttagc agaaaaaatc atttccactt gtcgggaaat gaatgcgtca ggtcttaatc
     2581 aaggaacatc aggtaatgtg agtgccaggt ataccggtgg catgttgatt acgccgagtg
     2641 ggatcgctta ttcaaagatg acgccggaca tgattgtctt tgtagacgat aagggaaaac
     2701 ctgaagcggg taaaatacca tcaagtgaat ggttaattca tctggcgtgt tataaagcca
     2761 gaccagaatt gaatgctgtc atacatacgc atgcggtgaa ttccacggcg gtggcaatac
     2821 ataatcattc aattccggcc attcattata tggtggcggt atcggggacg gatcatattc
     2881 cctgtattcc ctattatacc tttggcagtc ctgaactggc cgacggtgta tctaaaggaa
     2941 tcagagaaag taagtccttg ctgatgcagc accacggtat gctggccatg gacgtcacgc
     3001 tggaaaagac gttgtggctg gcgggtgaaa ccgagacgct ggctgattta tacatcaaat
     3061 gtggcggatt acatcacgat gttcccgtgc tgtctgaagc cgaaatgacc atcgtccttg
     3121 aaaaattcaa aacgtatggt ttgaaagcgt aattacagct ttattttaat taataacata
     3181 tgacagccat tcgcagaatg gctgctattc cctctgtcca caacgattga ttataactac
     3241 cacaaggagt tatttatgtt tatcgtggaa tcgtatgctg tcgccataat aatgtgtttt
     3301 attaccatga tttgctgggg ttcatgggcg aatacaacta aacttgtcag caataaaaaa
     3361 tgggagttcc ctctattcta ttgggattat tcaattggat tattgctgtg ttctttgtta
     3421 tttgccttca cgctgggttc tatgggcgaa gccggtcgca gttttatacc tgatattcag
     3481 caggcgagca gcagtagtct gatgtcggca atactggcag ggattatctt caatatttcc
     3541 aatattcttc tggtcgcttc tattaatctt gccggtatgg cggtagcttt tcctgtgggt
     3601 gtggggctgg cactggctct gggtgtgatc accacctata tcggcaatcc gcagggcgat
     3661 ccgctcattc tgttcctcgg tgtcgtctgc gttgtgatcg ccattatttt taccgcgata
     3721 gcctatggtc gtgtcacgca ggaggcggat aaatctcgcc gtaataaagg tctcattact
     3781 gccattctgg cgggtatcat catgggatgg tttttccgct tcctggcgga ctccatgtct
     3841 gacaatttca gccagcctgc cagcggtctg atgacgcctt actcagcact tgttctgttt
     3901 gccgtggggc tgtttttgtc gaactttgtc cttaacaggc tggtcatgaa aaaacctatt
     3961 tcaggagaac cggtcaacgg gaaaatgtat ttttccggga gtttacggga tcatgtttgc
     4021 ggctggctgg gcggtatgat ttggtgtgtc ggtctggcat tcagtctgat tgcgtctggt
     4081 caggcggggt atgccatttc ctacggtctg ggacaggggg ccacaatgat tgccgtgatc
     4141 tggggagtct tcatctggag agaatttgcc agtgcgccag ccggaaccaa taaactgctg
     4201 ctgaccatgt ttatttcata cattgtgggt attgttctta ttatcgccgc taatcaataa
     4261 aatcctgccc catatctaca tggggcagtt gttcattctt ttagtgtggt aattcacacg
     4321 ccagcaaaaa ctctgccgtt ccttcatcaa caatcaggtc cgtgacatat cctcccagca
     4381 gggcacccaa cgttgcgtca tagcctcttt ccccaccagc aaggaagatc ttccgttcaa
     4441 tctgccttag ctgagccaga ctgattccca gaatacgctg gtcaacatca gccacgacgg
     4501 gcatcccttc cttgtcatag aagcgaccac aaatgacacc tactgcgcct aaatcccgat
     4561 acgtctgcat ttccttctta ttcagcacgc ccacccgaat caggggattt tcatcaagcg
     4621 cgttacccac gacaaaaaag gctttcgtac agcgagtcag aacgtcaaaa ttcgatcgaa
     4681 taataggctc tgcctgtaac tccatggcca gtctggcgct tgataccacg gcgggcacgt
     4741 gcagcgaggc gacacggcct gaaagcttgt aggcgatttg cgatgaggat tcaatgatcg
     4801 tcaggtctgg ctgagatggc atggaaccga gcatctgtat aacggtgaca tttttacatg
     4861 acttcggcgt catggttttg ctcatctgat ggattgttct tccccaggca acccccagca
     4921 catcaccatt ctcaatcacc tgattcagat acatgccacc ggcacgtgaa aggcgagtcc
     4981 gcatcagctt tgtatcatca cgttttccgg catgttcgcc atccggcacg atgatcactc
     5041 tctggagatt gaatttatcg cgaatttgca aagcagcgtc gatagagcca aagacattca
     5101 catccaggtt gatatgaacc agtccatttt cccgggctgt ttgcaggtat ttgacaaccg
     5161 tgacgcggga cacccccatg atagctgcaa cttcagtctg gcttttacct tcctgatagt
     5221 acagccatgt tgcatgaagt acgggatcat cattgaacag gctgctgttt gtttgatctc
     5281 tatccacttt tgtatacctg tattaaaccg gggaaatgct catcttaacc ctgtttaccc
     5341 ggagagttga tgcagattgc cagtggcatg attttataga tatattttat tcttttacat
     5401 gaaataaacc agcataccat ttgagcgatc gcccgctgtt cccccatact gtaaagccgg
     5461 agacatgctc cggctttaca gtattactga gaatacaccc atcaagcata atcgaaggtt
     5521 caatatcgcc cctttttgat cgccagcgat tcgtcaccgt gcagacggtt gccgtcactc
     5581 actaaattat gggggacagt gaacaacagg ttggctcact ggcaagaacc cgattataaa
     5641 actaagctgg cgagcagaat aaaaatcagc ccactgatgg ataagagtgt ggacatcagc
     5701 gtccaggata acagcgtttc tttggtggtc aggccaaaaa agtctttaat catccagaaa
     5761 ctggcatcgt taacgtgaga acaaatacag gatccggcac cggtagccag cgtaatcaag
     5821 gccaggttgg tattgggatg tactgccaac aatgggatga ccagaccggc ggtagagatc
     5881 gccgcgacgg tggctgaacc cagacaaata cgcaggaatg cggccacgcc ccaggccatc
     5941 aggatcgggt taatatccat acccgaaacc agggtggaaa tatactgacc aacgccagag
     6001 tcgatgagaa cttgcttgaa tgcaccacca ccaccgataa tcagcagcag accggcaata
     6061 cccgcgatag cttttccgca agaatccatc aggtcaggga ttgttttccc gcgccccagg
     6121 cccatggtat agatcgcgaa tagcagtgaa atcagcatgg cgatcgttga attacctaag
     6181 aacagtatca cgttgtaaaa taagcctgaa tccgcggcac ttttggcatg agtcatctgg
     6241 atgatggtga caaccgccat caggatcacc ggcagcattg ccgtgagaaa actgatccca
     6301 aagcctggca tttcgctttc gctgaatcgg cgggtcgcgc cgagagaggc tatattcccc
     6361 tcttttttaa aggcgtccgg aataatgcgc tggcagaatt tattcaatac cgggccgcaa
     6421 agaataaagg ttggaatgcc gacaatgata ccgtatatca gtaccaatcc tacatcagcg
     6481 ccatattccc tggcgataac agtcgggccg gggtgcggcg gcaaaaaacc gtgtgcgacc
     6541 agcaaaccag aaagcatcgg cacacacata aacatcggtg atatttttgc ttcacgggca
     6601 atagcgaata aaataggtac cagaagaatt aaaccgactt cgaaaaaaag tgcgataccg
     6661 acaataaacg ccgaacagac cactgcccag tcaagtttat ttttcccgaa ataattcagc
     6721 atggtcagcg ctattcgctg cgcaccaccc gcatcggcca gcaagcgacc gagcataaca
     6781 ccgaagccaa atatcaaacc aatatggccc agcgtaccgc caagtccgga ttcgactgag
     6841 gtgacgactt tactcaaatc cataccactg gcgatggcga cagcaatcga gacaataatt
     6901 aacgaaacaa acgtattaag cttaattttt atggttaaaa gcaggagtaa agcaatccct
     6961 gccacaacga taattagtgg cataacgtcc tctcataaca gggtagcgac ccatcattat
     7021 ccgactgagc aaataacgaa tgatgcagag tcggataatt caggtgtctg gatgttttta
     7081 tttatgcttt cagtttttat tcataaaatc gcgcaaagcc gcgccaattt tgtttacatc
     7141 ataaatacaa ccggtccatg tcccaccact cacggcctga agcattgccc atagccgggt
     7201 atcatctggc aattcaggat cgggaagtaa atcctgatgg ctgggtctgg catttaatat
     7261 tgcagttgcc tcctcctgtg aaggtaattg ttcatcgcta cgggttccga ggaaattgac
     7321 ttcgccgtga agctcgcgac aatcaatttt aatttcaatt aaatccccgg tgcgtaattt
     7381 accgatgggg cctccggcca gcgcttctgg ccccacatgg ccgatgcacg cgccagtaga
     7441 aacgcccgag aaacgtgcat cggtgattaa cgaaacatgc ttaccgtatg acagatgctt
     7501 cagggcactg gtaacctggt aggtttcttc catccctgta cctgaaggtc caacgccaat
     7561 aatgaccaga atatcgcccg ccttgatctt gtcatgtttg atatcgtaaa tcgcactttt
     7621 ctcggacaga taaaccttcg ccacaccttt atggtaatag ataccttgct catcaatcat
     7681 cgaggggtca atggcggtgg atttgatcac cgaaccttct ggcgcaatat tgcccaccgg
     7741 gaaggtgata gttgaggtta atccgcgcgc ttttgcttgc tgcggagaca tgatcacttc
     7801 gtcagcgttg atttgttcct gatcgagcag gagttgcttg aaccgctgac gccgttcgga
     7861 gtgctcccac cagtcgaggt tttctttcag cgtgctgccg gtaaccgtca taacgtcttc
     7921 atgcaacaat ccgaggctgc gcagatgcaa catgacttcc ggcacaccac ctgccataaa
     7981 ggcattgacc gttggatgat aaaccgggcc attaggcagt acgctcacca gtcggggcac
     8041 gcgcttgttg atgcggatcc agtcatcaac ggtcgggata tggcaacctg cctggtgagc
     8101 aattgccggg atgtgtaaca gcaggtttgt tgaaccaccg aacgcggcat ggaccgtcat
     8161 cgcattctct atcgctttat cggtgagaat ttcccgggtg gtgatgcctt tttgactcag
     8221 gttcagcgca gctcgcgcgg aagctctggc gatctcccgc cacacaggct caccggaagg
     8281 ggccagggct gaatgtggga ttgccagtcc caatccttcg gccaccacct gagatgtccc
     8341 ggcagtgccc aaaaattgac agccgccgcc ggaagaggca caggctttac agcccgcacg
     8401 gcgtgcgtcc tgtagagata attcgccatt ggcgaagcgt gcgccaatgg tttgcacctt
     8461 gccgttgtct tctccatcct ttgcgggcag cgtcgcgccg ccggggacca gcacggttgc
     8521 gatgttgtgc tgcgcggcga gtgccatcat ggtggccgga agccccttat cgcaactcgc
     8581 cacaccaata actgctttcg cgtcgggcag agagcgaata aggcggcgca ttaccatcga
     8641 tgcgtcattt cggtatggta gcgaatcaaa catccccgtt gtaccctgag tacgcccgtc
     8701 acaaggatcg gagacgtaca cggcataggg cagggcatgg ttggctttaa taacctcggc
     8761 cgccgctttc atctggatgt ccagttcgta atgcccctgg tgcaacgcca gcgccacggg
     8821 tttaccgtct gcgccgcgta agccaccgag tgtactgagc agtaaaatac cgtcccgatc
     8881 cagctcgtcc ggagaccaac ccattccggc attcatggtc ataccgaaca gatccccgct
     8941 cgggcggttg ataagcatct ctgcggttaa tgggagttcg ccgtccgggc catcggcgtg
     9001 cgttctgacg gtgtaaatat cgtggctctc gtcagcaaaa atattgcgaa cagacatgag
     9061 tttctcctca gactggtaaa atgccctgcg cggtaagcag cacgtggact ttatcttttg
     9121 cttcttcaga tgcttcaaga atcggtggta agcaatatgt ctctacaggc agcccgacac
     9181 actgcatgct gtatttgatc agtgagacaa acggtgtttc gagggcgtaa atagcgggca
     9241 gttgtagtag ttttttattc agcgtcgcag cggtcgccag atcgccttca cgccaggcgc
     9301 ggtagatgcc gacggagagt tccggagcaa agttagcgct ggcggttatc gcaccgtcgc
     9361 cgcccagcag catcgtattc agcaaatgat catcgtaacc gcagaatacc gaaaacgacg
     9421 ggcgtaccga cttaactgtg ttgatcatcg tacgcaagtg accaacgctg tcgatggtgt
     9481 ctttgatgcc aacgatattc tcgttttgca gagccagacg cgtcacggtt tccggggtta
     9541 agtcctgacc cgtcagatcc ggaaagttgt acaggatcac cggtagggtg acgctacggg
     9601 cgatctgctg gtaatagtcg tcaagatttc gtggtgcgac tttccagtag taggggttga
     9661 tggcgacgat accatcagcg ccgtaggctt gcgcatgctg cgccagtttg accgcttcgt
     9721 cagtggaagg ggaaccgacg ccaatcaata ccggcactcg cccgtcgaca atggttacag
     9781 cttcttcggc gagtgccatg cgctgggctg tattcatttg gctaaattca ccaccggtac
     9841 ccagataaaa cagcccgtcg acccctttat taatcaggaa gtcggcaact tcgcgcattg
     9901 cctttttatc aagggttccg tcacgatgaa acgtgctgga taccggtgga ataatgccgc
     9961 tgaatttttt cattacctca gtttcctttt cgtaagtgct cattttcgtt tctgtatccc
    10021 agcccatatg atatttgctc agcgcatttc cggagcaatt ccagatagct gtctatctta
    10081 tttcctgaat aaactaccgg atcgccagaa agtgagattg catagttaac tctgttatac
    10141 atattgaata ccggcatact taaacatacc gccccatagg ttgattcttc gttgtctatg
    10201 gcccatcccc gaaggcgtgt tttttgtaat tcttccagaa acaatttttt atcggtaaat
    10261 gtattgcgcg tatgtggagt taacgtgagt gcttctaaaa aataatccag ttcttctcgt
    10321 gtcttccagg ctaataatgc cttacccaac gcggtaatat gcaactcaag ttttttgcca
    10381 atccagcttt tgctcgtcgg tacagagtcc ggactttcga tcttgtcgag atagatagcc
    10441 gaaatgctct ccatcgcccc aagatgacaa accagtccac ttttcagcga caactcctgc
    10501 attggccgct tagtcacctc aaatatattt tgacggtgta acgcctgaca acccaattcg
    10561 tagtttttta ttcccagtga atagtagccg tttttgtttt taattaagaa accacaatcc
    10621 accattacat tcaataaatt aagcaggctg cttttggggt actggaattt atttaataac
    10681 tccatataac ttgccatacc aacccatgca atgtgggtta atattttttc agcacggact
    10741 aatgaattac accctttgcg aaccataatg gctcctcctt gctcatggaa taaatggtat
    10801 cggcattaca tagtggaaaa tatgctttgt tcagcatgct gaaccgtaat gatgtgatta
    10861 aatgtgatct tgtacacact ttgaataatg aatccagaac agccacattt agtgaatgag
    10921 aataaaaagt agatggcgtg ttgttgcaac atcgtagtgt gtggattgcg cctagagctt
    10981 ctttattcgt tcgagcgcat cgccaatatt ttctattggg tgccattgat tatttttgct
    11041 tgaaatccct ctcgaatgaa cttacttatc actttattga atgagataaa aacaactgac
    11101 atccggctcg ctgataacgc tgtgcaatag tctcaggtaa cccgctgtca ctgatgatcg
    11161 ttttaataca tgatagtgtt gctacagcat gcggctcaac ggcatcaaat tttgagtgat
    11221 cggccatgag aataacttca cgcgagcgtt gaataatccg tgttttcacc ccgacctcaa
    11281 acatggtggc attggtgatc cccgtctcca gcgacagcgc atcgcaggag ataaacgcac
    11341 gttctacgga aaaggcattg atcatctcca gcgccagact ctctcctacc gaaaaataac
    11401 ctgggcgaat cagtcctccg ataatgtaac tttccacatg gggaaaacag cccagttcat
    11461 tggcgatttt aatgtcgttg cagatgacct ttacccgcgc atcggccagg catttcgcca
    11521 gttcgaggca ggttgagccg gagtcgagaa aaaagcagtc gccatctttg atcattgttc
    11581 gggcaagggc agctatttct cttttcgcca cagattgcaa tgtacgtttc acgtcgaaga
    11641 cgtactcttt gtcgtctgta ctgtcgtcga agtccaggca accgtgaccg cggatcatgc
    11701 ctggatactg gttgacaata tactggaaat cacgacggac agtcgcttcc gcatagccaa
    11761 aaagctccat ggcctgctgt gtggagaggt gacgatgttg ccacaggtag tgcagcatct
    11821 gtttgatacg gtcaggtctc tgttggctca tcagaaacaa tctcctctta cagggtggcg
    11881 aattgagcta aggtcgcgcg atagtcagat gaagagaaca gggcgcgccc aatcaccata
    11941 tgttgcgccc cagctgctgc cagttgctgt gcagctgcca gcgtaattcc accgtcggcc
    12001 cagcactcgg tctgaggaaa agccgtgcgt actttttgga ttttctcgca catggaggga
    12061 ataaaccgct gtccctgccc atcgggctca ctggtcatca ccatcactcc gtccagctca
    12121 gacgccagat atcgccaggc gtcgatcggg gttgccggat taaataccag tccggctcgt
    12181 gcgccggtat ggcggatctc agttaacgtt tccgaaggat agtccagcgt ttctgcatga
    12241 acgaagatcc acgcgggtct gatttcagcc agcgcgttaa accagcgctg tggccgggca
    12301 agcatgaaat gaaaggacaa gggatgaggc gtctgtctgg cgacggcctg caccgttttc
    12361 atcccaaacg taatgttgtt aatgaagctg ctgtcctcaa tatccagatg caggctccca
    12421 aaatccagat tgtccaatgc cgtcagctca cgaccatagt gcagaggatt tgcgctggcg
    12481 agagaagggt gcaggatcat aagcgcctcc gttaacctga taccagcgca aacagtgcat
    12541 cgtctgtgct ggcatttccc actgccgtta agcgttgtgg cgaatcaata aactggctga
    12601 tgcgctgaat ggtcaaaata tgtgcgttgg catccgtggc gctgacgcag agaagcagcc
    12661 agacggggtc gcactcctcg ttaccaaagg cgaccggggt gcgtagcgtg gtgatactga
    12721 cctgattata gtttgcgcct tgctctggtc gggcgtgagg caaggcgatt cctggggcta
    12781 tcagataata aggcccccag ttaagggtat tttcgatgat cccattaacg tagcatggct
    12841 gcgcggcacc ataggctacc agcgggcggg tggcgatttc cacggcctga cgccagtcgg
    12901 tggctttacg ttgtgcctgt acccatttaa tatcgttaat catccgtcgt tgactccatg
    12961 ccgattcggg ttaatctggt agcgatcccc gtcgatactt ttgacgaagg cggcagggat
    13021 cgcagaaggg ttattgctct ttgccgggga taagcgctct gcttatcccc atcttatcgg
    13081 cgtatatgat gcactttctc cataaactga ctgactcgcg cctggtcaac gaaattggca
    13141 aaaacgccgt cctttttaaa ggtggttgcg gtcacacagc catcggcgat actgagttgt
    13201 tcctccacgt tttccaggca gacgcccgtg ttggccaaca cgacggtgtc aggcaccgtt
    13261 tctttgaccc gttttaacag cgcgctgtcg gtacgcgtcc cggcggtcag gccagaaaca
    13321 cagagcgcat caggatggtt gttaaatact gtcgatttag caatagaaca gatatcgcga
    13381 ttgcccagat acacggcggc ctctgggacg atattgaaca aggttttcac ttcgcctgca
    13441 ccaatccggt gttgatgacg gatggtctca ccgacgttag tgtcccagac gccaaagtcg
    13501 ctggcatagg caccggtaaa tatctcgcgg ataaacttag cgccggtagc catcgccagg
    13561 tcgaatgagg cgaccggatc ccacagaaca ttcacgccaa acgggatgcg gatatcgctc
    13621 attaattgcc cgatgattcg tgccattgcc gcagtggttt ccggacgcac cttcgtgaga
    13681 tagggaagac taaactcgtt ggagaacatg actgcatcca ccccaccatt ttggagcgcc
    13741 atcagatcgt cccaggcttt gtcgataacc cagttcatcc ccaactgcgc atcaaaactc
    13801 gggtcgccgg gcaatgcacg tagatggcac atggcgatga ccgctttttc cgtcccaata
    13861 acctctttca gccaactcat tgaaagtact ccttattccg ataattcacg tttacgtaag
    13921 aacagcacga cgctggcgac aataccgacc gccacgacaa cgccaataat cccaagcgac
    13981 atcaattcag aaatcgacca accgaacata ttgcccacgg acaacgctga gatttgcgca
    14041 ctttcaccgg caaaactgaa accgcctttg agcgccattt cagtgaagta aggcgcgaac
    14101 tgggtggcga tcagcaggac ggtaatcatc acgatgaccc cgcttatcaa cgtcctgacc
    14161 aggtctccgc ggtggataac cgtagccata cagataaaga acggtgctac cggcaggtct
    14221 gcgaggggta acactttgtt gcccggtaaa atgctggcca gaattaacat aatgggtatc
    14281 agtagcagac cgacagcaat ggtggttgga tgccccagcg tcacagcagt atccagaccg
    14341 atatacactt cacgtccttt gaaatatttc tggaagaact ttcttgcacc gtcggagatg
    14401 ggcagcaggc cctcaacaat caggcggatc atgcgcggga acaacaccat gattgccgca
    14461 acggtaatca tcagactggc gcaaccttta aagccttcac cagccgccag gccaaaaatc
    14521 agaccgagca ctacgccgat aatcaccgga tcgccgacca tgccgtagcg tttctggatc
    14581 tcctgagcat cgatattgcg tcccttcatg aaagggattt tttcatagat agcatcaagt
    14641 agaacgaaaa gcggtactga actggaaccg tagccttgcg gtatcgaaat accttccagc
    14701 ccgacgatgt tctgtacccg ttttgccgtc cagtcggcca tttttaacga tagcgccgca
    14761 tggcagattg cccccagcac accataaatc aggctaccgg tcataagctg gaccaccgta
    14821 cccgtaatgg cgtagtgcca gtaattgtaa atatcgacgt tcatcgtttt ggtcagacgg
    14881 gtgaccagca tggcgacatt gagaagaaaa ataactggga taatcatggc gccaatggct
    14941 gtcgcataac caacgccgga ggcagggcct gcgccaacgt caaagacatg cagggctagg
    15001 ccgaaacgct caatcatcac tttgataggg ggactgaggc tgtcgatcgc catgacgatc
    15061 accagtccca taccgacaaa accaatccca accgttaccc ctgccttaat cgcctgcagc
    15121 caggggatgc ggaagataag gccaatcacg atcatgatga tagggacgaa aacggtaccg
    15181 ccgagagaca ggatgtaatc aaacataatg cctcctttta ttgagttaac agagccttga
    15241 tttgttgttt taacgcgtcg tcgttgatcc ctgtgagcag ggcggcgcca ttaagcgtag
    15301 ggatgccgta atcactgttg gttcgcatgg aggttacaat aaggtccata ccgttacagt
    15361 taagagggat ctcattcaga cagcattggg cggtagtggc tgaaatgccc tgttcagtca
    15421 ggaactcttg cagcttgtgt gcaatcatgg ttgaagtcga catgccggta ccgcatgcca
    15481 caaggatctt tttcataatg gatgtgcctc ttgtgtagtg cctgaatcag gctcaacggg
    15541 gaaatgtgct gctgacatac ctgccagagc ggtcaatagg cggatgcaat cagtcaaatc
    15601 acgcaggctg gcaacctccg ccggagaatg ggtatagcga caaggaatag agagactggc
    15661 gcagggaata ccatcctgct caacctggat ataaccggtt tctgttatca cgccgggagc
    15721 cacttctcgc tgcaccggaa tgttgtgttc aagagccgtc tgttccaaca tccgtatcag
    15781 gcgaggaggc gtgattaatc cggccagcgt tccccgacca tggtagttca ggcaggtgat
    15841 cccaacgccc tgattaatcc tgacctcgga ataatcgtgt aaatcagggg tgtcacacga
    15901 tggagtgata tcaataccaa tcgc
//
LOCUS       DAJOWB010000107        15769 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJOWB010000107 DAJOWB010000000
VERSION     DAJOWB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.107"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            193..435
                     /locus_tag="OHO85_003661"
     CDS             193..435
                     /locus_tag="OHO85_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672982.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            549..1187
                     /locus_tag="OHO85_003662"
     CDS             549..1187
                     /locus_tag="OHO85_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCQ2672983.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1184..1921
                     /locus_tag="OHO85_003663"
     CDS             1184..1921
                     /locus_tag="OHO85_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCQ2672984.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1921..4017
                     /locus_tag="OHO85_003664"
     CDS             1921..4017
                     /locus_tag="OHO85_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCQ2672985.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4064..4342)
                     /locus_tag="OHO85_003665"
     CDS             complement(4064..4342)
                     /locus_tag="OHO85_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672986.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4343..4486
                     /locus_tag="OHO85_003666"
     CDS             4343..4486
                     /locus_tag="OHO85_003666"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2672987.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4612..5022
                     /gene="psiE"
                     /locus_tag="OHO85_003667"
     CDS             4612..5022
                     /gene="psiE"
                     /locus_tag="OHO85_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCQ2672988.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5066..6541)
                     /gene="xylE"
                     /locus_tag="OHO85_003668"
     CDS             complement(5066..6541)
                     /gene="xylE"
                     /locus_tag="OHO85_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCQ2672989.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSSRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSILQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6913..7803)
                     /gene="malG"
                     /locus_tag="OHO85_003669"
     CDS             complement(6913..7803)
                     /gene="malG"
                     /locus_tag="OHO85_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCQ2672990.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7818..9362)
                     /gene="malF"
                     /locus_tag="OHO85_003670"
     CDS             complement(7818..9362)
                     /gene="malF"
                     /locus_tag="OHO85_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCQ2672991.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9516..10706)
                     /gene="malE"
                     /locus_tag="OHO85_003671"
     CDS             complement(9516..10706)
                     /gene="malE"
                     /locus_tag="OHO85_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCQ2672992.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11071..12186
                     /gene="malK"
                     /locus_tag="OHO85_003672"
     CDS             11071..12186
                     /gene="malK"
                     /locus_tag="OHO85_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCQ2672993.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12258..13598
                     /gene="lamB"
                     /locus_tag="OHO85_003673"
     CDS             12258..13598
                     /gene="lamB"
                     /locus_tag="OHO85_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCQ2672994.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13841..14761
                     /gene="malM"
                     /locus_tag="OHO85_003674"
     CDS             13841..14761
                     /gene="malM"
                     /locus_tag="OHO85_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCQ2672995.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14990..15769
                     /locus_tag="OHO85_003675"
                     /pseudo
     CDS             14990..15769
                     /locus_tag="OHO85_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579104.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT         3964 a         3967 c         4089 g         3749 t
ORIGIN      
        1 ttattttaac attatgattt ttaatgttat ttatatataa gttgtagctt atatttgata
       61 ttcattccaa ttatcctaaa accccatctc taattccccg cgccgtaatt cgcatgcttt
      121 agttgtgtat actcgatccc gcccgaaatg tttttgggta aatctccatt cattcaatga
      181 agggaaattg ttatgaaaaa agttctgtat ggcatttttg ccatatctgc gcttgcggcg
      241 acttctgcgt gggctgcacc tgtacaggtg ggcgaagcgg cagggtcggc agcaacgtcg
      301 gtttcggcgg ggagttcctc cgcgaccagc gtcagcaccg taagctcggc ggtgggtgtc
      361 gcgctcgcgg caaccggtgg cggtgatggt tctaacaccg ggaccaccac aaccacgacc
      421 accagtaccc agtaataaag tatgtatccc caaaataatt tgagtcattg catctgtggc
      481 ttgaagtatg aagggattta ccataaccac actccggtgt ggttattctg cccctctgga
      541 gaagagtcgt gaagcgacct gcactcattc ttatctgcct gctattacag gcctgttcag
      601 ccacgactaa agagctgggc aattcactgt gggacagtct gttcggcacg ccaggcgtac
      661 agctgacgga cgatgatatt caaaatatgc cctacgccag ccagtacatg cagcttaatg
      721 gcgggccgca attgtttgtg gtgctggcct tcgctgaaga cggacaacaa aaatgggtca
      781 ctcaggatca agccactctt gtcacacaac atggtcgtct ggtgaagact ttgcttggcg
      841 gcgacaacct gattgaagtg aataaccttg ccgccgaccc gctgattaaa cccgcacaaa
      901 ttgttgatgg tgcaacatgg acgcgcacga tgggctggac cgagtaccag caggtacgct
      961 acgccaccgc acgctcagtc ttcaaatggg atggcaccga taccgtcaag gtcggcagcg
     1021 atgaaacccc ggttcgcgtg ctggacgaag aagtctccac cgaccaggcg cgctggcata
     1081 accgctactg gatcgacagc gaagggcaaa ttcgccagtc ggaacagtat ctcggcgcgg
     1141 attatttccc ggtgaaaacc actctcatca aggcggcaaa acaatgatta aacaaactat
     1201 tgtcgcattg attttgagtg tgggggcgtc atcggtcttc gcggcgggaa ccgtcaaggt
     1261 attcagcaat ggcagcagtg aggccaaaac gctgacgggc gcagagtatt taatcgatct
     1321 ggtaggccag ccgcggctgg cgaatagctg gtggcctggt gcggtgatta gcgaagagct
     1381 ggcaacggcg gcagcattgc ggcagcagca ggcgttgctg acacggctgg cagaactggc
     1441 tgcagattcc agcgccgacg atgccgccgc gattaacgcc ttacgccagc aaattcaggc
     1501 gttgaaagtg acgggcaggc aaaaaatcaa tcttgatcct gatatcgtcc gcgttgccga
     1561 acgcggtaac ccaccgttgc agggcaacta cacgctgtgg gtcgggccac caccgtccac
     1621 ggtcacgctg ttcgggctta tcagccgtcc tggcaagcag ccattcactc caggtcgcga
     1681 cgtggcgagc tatctctctg accagagcct gctcagcggt gcggatcgca gctacgcgtg
     1741 ggtcgtttac ccggacggac gcacacaaaa agcgccggtg gcttactgga acaagcgtca
     1801 cgtagagccg atgcccggca gcattattta tgttggcctc gcggactccg tctggagtga
     1861 gacgcctgat gcccttaacg ccgacattct tcagactctg acgcagcgga taccccaata
     1921 atgaaaaaaa gacatctgct tagcttactg gcgctgggca ttagcacagc ttgctacggc
     1981 gaaacatatc ctgcgcccat tggtccgtcg cagtcggatt tcggtggcgt aggattatta
     2041 caaacgccca ccgcgcgcat ggcgcgggaa ggggagttga gcctgaacta tcgagataac
     2101 gatcagtacc gttattactc agcttcagtg caactcttcc cgtggctgga aacaacgctg
     2161 cgctacaccg acgtgcgcac ccggcagtac agcagcgtcg aagcgttctc tggcgatcaa
     2221 acgtataaag ataaagcctt cgatctcaaa ctgcgtttgt gggaagagag ttactggctg
     2281 ccgcaagtgg cggttggtgc gcgggatatc ggcggtacgg ggctgtttga tgcggaatat
     2341 cttgttgcca gcaaagcctg ggggccgttc gattttacgc tcggcctggg ctggggatat
     2401 ctgggcacca gcggtaatgt gaaaaatccg ctctgttcag ccagtgataa atattgctat
     2461 cgtgataaca gctacaaaca ggcgggatct atcgacggta gccagatgtt ccacggtcct
     2521 gcctcactgt ttggcggcgt ggaataccag acgccctggc aaccgctgcg cctgaaactg
     2581 gagtatgaag gcaataatta tcagcaggat tttgccggga agctggagca aaaaagtaag
     2641 tttaacgtcg gtgcgattta tcgcgttacc gattgggccg acgttaacct tagctatgaa
     2701 cgtggcaaca cctttatgtt tggcgttacg ttgcgcacca actttaacga tctgcgcccg
     2761 tcttacaacg ataacgcccg cccgcaatat caaccgcagc cgcaggatgc cattttgcag
     2821 cattcggtgg tggcgaatca gttaacgctg ttgaaataca atgccggact tgccgatcca
     2881 cagatccagg cgaaaggcga tacgctgtat gttaccggcg agcaggtgaa atatcgtgat
     2941 tcgcgcgaag ggatcatccg tgctaatcgg atcgtgatga acgatctgcc ggatgggatc
     3001 aaaacgatcc gcattacgga aaatcgcctt aacatgccgc aggtgacgac ggaaacggat
     3061 gtcgccagcc tgaaaaatca tctcgccgga gagccgttgg gccacgaaac gaagctggcg
     3121 caaaaacgcg tcgagccagt ggttccgaag tccaccgagc agggctggta tatcgacaaa
     3181 tcgcgctttg atttccatat cgatccggtg ctgaaccagt cggtcggtgg cccggaaaac
     3241 ttttacatgt atcagctggg cgtgatggga acggcagatt tgtggctgac ggaccatctg
     3301 ctgaccaccg gcagcctgtt tgcaaatctt gccaacaact acgacaagtt taactacacc
     3361 aatccgccgc aggactcgca cttaccgcgc gtgcgtaccc atgtgcgcga gtatgtgcag
     3421 aacgatgtct atgtgaataa cctgcaagcc aactacttcc agcatctggg caacggcttc
     3481 tacggtcagg tctacggcgg ctatctcgaa accatgtttg gcggtgcggg ggctgaggtg
     3541 ttgtatcgcc cgctggacag caactgggcg tttggtctgg atgccaacta cgttaaacag
     3601 cgcgactggc gtagtgcaaa agatatgatg aaattcaccg actacagcgt gaaaaccgga
     3661 catctgaccg cctactggac gccatctttc gctcaggatg tgttagttaa agccagcgtc
     3721 gggcagtatc tggcagggga taaaggcggc acgctggaga tcgccaaacg ctttgatagc
     3781 ggtgtggtgg tgggtggcta tgccacgatc acgaatgttt cgaaagagga gtacggcgaa
     3841 ggggacttca ccaaaggcgt gtatgtctcg gtaccgttgg atctcttctc gtctggcccg
     3901 acacgcagcc gtgcggcaat tggctggacg ccgctgacgc gtgacggtgg tcagcaactt
     3961 gggcgtaagt tccagttgta tgacatgacc agcgaccgta gcgtaaattt ccgctaagtc
     4021 atgggaaagg tgccagtttt cgcactcact ggcaccttca ttcttaatgg cctgtcgagc
     4081 catcaggaac gcagttcacc cccggtttgc gatctggtgt gtaaaggcga aaacattctg
     4141 tcttcctgtc cgtcgatatt ggttgcaggt ctggttgcct gagcgactgg gccatcattg
     4201 ggctggagac attgctttgc ggtaggtcgg ctttatcagc agtaccgagc ggaccagcat
     4261 aagcaggaag aacagagact gataacatca aagcagcaaa ataaggcttc atttttacca
     4321 cctttatcag gttacgtttc atttgttcca gaggaacatt gtcgattttt cgcgcattgc
     4381 tggtggctgg gaatcacctg aatgggtgat ttttgaatta ccggctttgg tgcggtttgt
     4441 cttgccggat gcgccgccag gcgcggctta tccggcctac gggtaggtat atccggcttt
     4501 ggtggaggcg cgctccaaat ccaggttgaa caaaacatac acaaaaaata tagatctccg
     4561 tcacattttt gcgttataca ggaagctcgc cactgtgaag gaggtactgc tatgacgtca
     4621 ctctctcgtc cgcgcgtgga gtttatctcc accattttgc agaccgtact caatcttggc
     4681 ctgttgtgcc tcggcctgat tttggttgtc ttcctcggca aagaaacggt gcatctggct
     4741 gatgtgctat tcgcgccaga acaaaccagc aaatatgagc tggtagaagg actggtggtt
     4801 tactttctct atttcgaatt tatcgcgctg attgtgaagt actttcagtc cggttttcac
     4861 ttcccgctac gttactttgt ctatattggg atcaccgcca ttgtgcgctt gatcatcgtc
     4921 gatcataaat cgccactgga tgtgttgatc tactcggctg cgatcctgtt actggtgatc
     4981 accctgtggc tgtgtaattc gaaacggctg aagcgggagt aaaaagacag cacgccgaac
     5041 tggcgcggcg tgctggacag gaagattaca gcgtagcagt ttgttgtgtt ttcttcgttt
     5101 ccggttccca gagggcttcc agctcctcaa gggttttacc tttggtttcc gggacaaatt
     5161 tccacataaa tagtgctgcc agaacgccca tacacccgta aatccagtag gagaaaccgt
     5221 tgtggaaatg ggccaccagc caggagtttt tgtccatcat cgggaaggtc caggagacga
     5281 agtagttcgc cagccactgg gccgccaccg cgattgccag cgctttacca cgaatagcat
     5341 tcgggaagat ttccgacagc agtacccagc ataccggacc ccaggacatg gcaaaggcgg
     5401 caacatagaa cagcatcgac agaagcgcca caatacccgg tgcctgagtg taaaacgcgg
     5461 taccgaggct aaacataccg attgccattc cgagtgcgcc gataatttgc agtggcttac
     5521 gaccaaattt atccaccgtc ataattgcca gaacggtgaa ggtgaggttg ataactccga
     5581 caataatggt ctgcaacagc gcgatatccg tgctggcccc cagcgttttg aacacttccg
     5641 gcgcgtagta cagcaccaca ttgatgccga caaattgctg gaggatggag agcataacgc
     5701 cgatgacaat cacgcctacg ccaaacatca gcagacgacc accggttttg cggccatgat
     5761 ccagagagtg tttaatttcc tgtactgcct gagttgcaag ggtgttgccc ataattttgc
     5821 gcaggatacc ttccgcctgt tcttgcttgc cgcgtgacat cagccagcga ggactttctg
     5881 gcacggtata cagcagcatt aagaacagca gtgcagggat acattccgag gcaaacatat
     5941 aacgccagcc gtcagtattc agccagctgg cgtcgccgga acgggcaata aaatagttta
     6001 cgcagtaaac taaaagctgc ccgaaaataa tcgcaaactg gttaaaagag accagtttcc
     6061 cgcgaatatg agcaggagca agttccgcaa tatacattgg tgaaagcatt gaggctaaac
     6121 caacgccaat accgccaata atgcgataaa taacaaattc cgggacataa cctgccagat
     6181 aaacaggcac tgtgttgtcc gggtttatag aggtaaaacc aagttctggc caggcagaac
     6241 ctacaccaga aataaaaaac aggacagcag caatcttaag tgaatcacga cgaccgaagc
     6301 ggctactgca ataaccaccg agggcaccgc cgatgatgca accaatcaga gcgctggcca
     6361 cgcaaaaccc taacagggag ttggcagcgg attcacttaa gttttgtgga gcaacaaaga
     6421 cggtattgag tgactcaaca gtaccggaaa taacggcggt gtcgtagcca aataataaac
     6481 cacctaatgt agcgactaag gtaatcgaaa atatataact ggaattatac tgggtattca
     6541 ttcagacctg ccttagacca ttctgatctt ttatcagtgc attgatgttg gaattgtcag
     6601 gcagttaagg caattatcat tttttacaat gcgtttacgt aatgtctttt ctgtgatctt
     6661 aattgtgata attatcaaaa attgatgaaa acaatactat tgccgtgact cagagcacga
     6721 aagagaatta tcgtaagtgg gaaaacaaat aacgtaaaaa taataagttc tatgatggaa
     6781 atattaaccg gcgaacgatt cagattgcag acgaaagaaa aaaaggcgct ccgtggagcg
     6841 ccgaataaca gtcacaagtt gggataacgt aagttgaggg tgcagcggca taacattggc
     6901 agaacaacat ctttaacctt tcacaccacc tgccgtcagg ccgttgacca gccagcgttg
     6961 agccagcaag aagacgatgg tgatcggtaa tgcagacatc acggcagcgg cggcaaagtc
     7021 accccacagg tagttttgcg ggttgaggta ttgctgcatc cccacggcca gggtgtagct
     7081 gtttacgtca cgcagtaaca gcgacgcgac cggaacttca gtaatggcag cgataaacga
     7141 caggatgaac accaccgcca gaatcggtac tgacagcggc aacaggacaa ggcggaaggc
     7201 ctgccacggt gtcgcaccat ccagcgccgc cgcttcttcc agcgaactgt cgatggtttc
     7261 gaaatagcct ttgatggtcc agacgtgcag cgcaatccca cccagatacg cgaaaattac
     7321 gccgccgtga gtattcaggc caatgaatgg aatgtactca cccagacgat caaacaacgc
     7381 atacaacgcg accagtgaaa gtactgccgg gaacatctgg aaaatcagca ttcctttcag
     7441 cagcgtcgct ttgcctggaa agcgcatacg ggcgaaagcg taggcgcagg tggtggagag
     7501 cgccacaatg ccaatcgcgg aaatcccggc gacctttacc gagttccaca gccacagcag
     7561 taccgggaat ggcggtggcg taatgcgacc atcagcctgt tccacgctaa aacctaatgc
     7621 cagtttccag tgatcccagg agatttgctc cgggatcagg ctaccggtcg caaagttccc
     7681 ctggcgcagt gagatggcga cgaccatcag cagcgggaac ataatcgccg cgataaaaag
     7741 tagcagtagc aggtgagtaa taaacaaacg tgctttttgc gatttcggtt ggaccattgc
     7801 catttttgtt atctccctta atcaaacttc attcgcgtgg ctttcaggtt cactatcgcc
     7861 agcgcaccca ccagcaggaa gatcagcgtg gctattgctg ctgccagacc gaagtcctga
     7921 cccccgccgc cttcaaaagc gatgcggtag gtgtagttaa caagcaggtc ggtataaccg
     7981 gctggcgtgg tcgtgccaag acgatccggg ccgccgttgg ttaacagttg aatcagcacg
     8041 aagttgttaa agttaaaggc gaagctggcg atcatcagcg gcgtcagcgg tttaatcagc
     8101 agcggcagcg taatcttaaa gaagttctgg aagggacctg caccatccat tgctgaggct
     8161 tcatacaaat cgtccggaat cgctttcagc aagcccatgc agaggatcat catgtacgga
     8221 taacccagcc aggtattgac gataatcagc atcgttcggg cggtggtcgg atcgctgaac
     8281 caggcgggct tcacgccaaa cagcgcgctc aacatcatgt tgatctcacc gaagctctgg
     8341 ttaaacaacc ctttgaaaat caagattgaa atgaatgatg gcaccgcgta gggcagaatc
     8401 agcaggacgc gatagaccgc tttgccgcgc aacgcttccc actgcaccag acacgccaga
     8461 accatgccga ccgccaccgt taaaaagaca gtgatcagcg agaacaccac ggtccagacg
     8521 aaaatggcga ggaacggttt ctgaatgcct tcgtcggtga agacgcgggt aaagtttttc
     8581 cagccagtag tcacggtgta accggggctt agcttttcat cgccccagtt gccgtcggcg
     8641 gtaatggact ggtaaaagcc aatttggtta ttcggacgat atttcacgcc gctctgatta
     8701 ttcgtcaacg tgccgtcacc gtcgagtgtg tagagcggct gcgtgccaga aaactggcgc
     8761 agggagctca tcatcacttt gttgccatcc ggcagaatgg cggtaatgtc actcagcgcc
     8821 tgacgattct gggtaatcac gcgcagattt gcgcgttcgc cttcgggctg ggcggtcgtt
     8881 tctttcagtt gcagtttttg ctcgccgcca aatttaaaag cgtcggagag gtaatttttg
     8941 ccggtttcgc cgtcgctgag ggctagttgc cactcatcgc ccgccgggta aagaccaaaa
     9001 ttataggttt tgcctgcttg ccaggagcga tctaacaaca cttcctgcgc acgttcaaaa
     9061 gtcagctggt tagtgctgct gtagttggtg aaggcaatgg cgatggtgca gaccagaggg
     9121 aagaggacga ataatcccat tccggccatt cccgggtaaa catagcgcca ggcgtaggct
     9181 ttacgattgg cgaaaatata cagccccgct gaactcaata tcagcgtggt aatggcaaac
     9241 aggtattccc cttgtgcgta cattaaaaca acaaggtaac ccaccagcag gccgagcaga
     9301 cctagcactg accatttcag cgcgtcgctt tgccaccaat gtttcttttt aatgacatcc
     9361 atggggttct tcctcattcc aggacggata aggctttcac gccttatccg acaacaactg
     9421 cctgatgcga cactgatgcg tcttatcagg cctacatacg tttcggtttt gtaggccgga
     9481 caaggcgttc acgccgcatc cggcatttca cagcattact tggtgatacg agtctgcgcg
     9541 tctttcaggg cttcatcgac agtctgacga ccgctggcgg cgttgatcac cgcagtacgc
     9601 acggcatacc agaaagcgga catctgcggg atgttcggca tgatttcacc tttctgggcg
     9661 ttttccatgg tggctgcaat acgtggatct ttcgccaact cttcctcgta agacttcagc
     9721 gctacggcac ccagcggttt gtctttatta accgcttcca gaccttcgtc agtcagcaga
     9781 tagttttcga ggaactcttt cgccagctct ttgttcggac tggcggcgtt aataccggcg
     9841 ctcagcacgc caacgaacgg tttagatggt tgacccttga aggtcggcag taccgttaca
     9901 ccataattca ctttgctggt gtcgatgttg gaccatgccc acgggccgtt gatggtcatc
     9961 gctgtttcgc ctttattaaa ggcagcttct gcgatggagt aatcggtgtc tgcattcatg
    10021 tgtttgtttt taatcaggtc aaccaggaag gtcagacccg ctttcgcgcc agcgttatcc
    10081 acgcccacgt ctttaatgtc gtatttgcca ttttcatact tgaacgcata acccccgtca
    10141 gcagcaatca gcggccaggt gaagtacggt tcttgcaggt tgaacatcag cgcgctctta
    10201 cctttcgctt tcagttcttt atccagcgcc gggatctctt cccaggtttt tggtgggttc
    10261 ggcagcagat ctttgttata aatcagcgat aacgcttcaa cagcgatcgg gtaagcaatc
    10321 agcttgccgt tgtaacgtac ggcatcccag gtaaacggat acagcttgtc ctggaacgct
    10381 ttgtccgggg tgatttcagc caacaggcca gattgagcgt agccaccaaa acggtcgtgc
    10441 gcccagaaga taatgtcagg gccatcgcca gttgccgcaa cctgagggaa tttctcttcc
    10501 agtttatccg gatgctcaac ggtgacttta attccggtat ctttctcgaa tttcttaccg
    10561 acttcagcga gaccgttata gcctttatcg ccgttaatcc agattaccag tttaccttct
    10621 tcgattttgg cgagagccga ggcggaaaac atcatcgtcg ttaatgcgga taatgcgagg
    10681 atgcgtgcac ctgtttttat tttcataatc tatggtcctt gttggtgaag tgctcgtgaa
    10741 aacacctaaa cggactctag tttctttata cggcaacctc tttccatcct ccttgcccct
    10801 acgccccacc gttgctttgt gtgatctctg ttacagaatt ggcggtaatg tggagatgcg
    10861 cacataaaat cgccacgatt tttgcaagca acatcacgaa attccttaca tgacctcggt
    10921 ttagttcaca gaagccgtgt tctcatcctc ccgcctcctc ccccataaaa aagccagggg
    10981 gtggaggatt taagccatct cctgatgacg catagtcagc ccatcatgaa tgttgctgtc
    11041 gatgacaggt tgttacaaag ggagaagggc atggcgagcg tacagctgca aaatgtaacg
    11101 aaagcctggg gcgaggtcgt ggtatcgaaa gatatcaatc tcgatatcca tgaaggtgaa
    11161 ttcgtggtgt ttgtcggacc gtctggctgc ggtaaatcga ctttactgcg catgattgcc
    11221 gggcttgaga cgatcaccag cggcgacctg ttcatcggtg agaaacggat gaatgacact
    11281 ccgccagcag aacgcggcgt tggtatggtg tttcagtctt acgcgctcta tccccacctg
    11341 tcagtagcag aaaacatgtc atttggcctg aaactggctg gcgcaaaaaa agaggtgatt
    11401 aaccaacgcg tcaaccaggt ggcggaagtg ctgcaactgg cgcatttgct ggatcgcaaa
    11461 ccgaaagcgc tctctggcgg tcagcgtcag cgtgtggcga ttggccgtac gctggtggcc
    11521 gagccaagcg tatttttgct cgatgaaccg ctctccaacc tcgatgctgc actgcgtgtg
    11581 caaatgcgta tcgaaatctc ccgtctgcat aaacgcctgg gccgcacaat gatttacgtc
    11641 acccacgatc aggtcgaagc gatgacgctg gccgacaaaa tcgtggtgct ggacgccggt
    11701 cgcgtggcgc aggttgggaa accgctggag ctgtaccact atccggcaga ccgttttgtc
    11761 gccggattta tcggttcgcc aaagatgaac ttcctgccgg taaaagtgac cgccaccgca
    11821 atcgatcaag tgcaggtgga gctgccgatg ccaaatcgtc agcaagtctg gctgccagtt
    11881 gaaagccgtg atgtccaggt tggagccaat atgtcgctgg gtattcgccc ggaacatcta
    11941 ctgccgagtg atatcgctga cgtcatcctt gagggtgaag ttcaggtcgt cgagcaactc
    12001 ggcaacgaaa cccaaatcca tatccagatc ccttccattc gtcaaaacct ggtgtaccgc
    12061 cagaacgacg tggtgttggt agaagaaggt gccacattcg ctatcggcct gccgccagag
    12121 cgttgccatc tgttccgtga ggatggcact gcatgtcgtc gactgcataa ggagccgggc
    12181 gtttaagcac cccacaaaac acacaaagcc tgtcacaggt gatgtgaaaa aagaaaagca
    12241 atgactcagg agatagaatg atgattactc tgcgcaaact tcctctggcg gttgccgtcg
    12301 cagcgggcgt aatgtctgct caggcaatgg ctgttgattt ccacggctat gcacgttccg
    12361 gtattggctg gacaggtagc ggcggtgaac aacagtgttt ccagactacc ggtgctcaaa
    12421 gtaaataccg tcttggcaac gaatgtgaaa cttatgctga attaaaattg ggtcaggaag
    12481 tgtggaaaga gggcgataag agcttctatt tcgacactaa cgtggcctat tccgtcgcgc
    12541 aacagaatga ctgggaagct accgatccgg ccttccgtga agcaaacgtg cagggtaaaa
    12601 acctgatcga atggctgcca ggttccacca tctgggcagg taagcgcttc taccaacgtc
    12661 atgacgttca tatgatcgac ttctactact gggatatttc tggtcctggt gccggtctgg
    12721 aaaacatcga tgttggcttc ggtaaactct ctctggcagc aacccgctcc tctgaagctg
    12781 gtggttcttc ctcttttgcc agcaacaata tttatgacta taccaacgaa accgcgaacg
    12841 acgttttcga tgtgcgttta gcgcagatgg aaatcaaccc gggcggcaca ttagaactgg
    12901 gtgtcgacta cggtcgtgcc aacctgcgtg ataactatcg tctggttgat ggcgcatcga
    12961 aagacggctg gttattcact gctgaacata ctcagagtgt cctgaagggc tttaacaagt
    13021 ttgttgttca gtacgctact gactcgatga cctcgcaggg taaaggtctg tcgcagggtt
    13081 ctggcgtcgc gtttgataac gaaaaatttg cctacaatat caacaacaac ggtcacatgc
    13141 tgcgtatcct cgaccacggt gcgatctcca tgggcgacaa ctgggacatg atgtacgtgg
    13201 gtatgtacca ggatatcaac tgggataacg acaacggcac caagtggtgg accgttggta
    13261 ttcgcccgat gtacaagtgg acgccaatca tgagcaccgt gatggaaatc ggctacgaca
    13321 acgtcgaatc ccagcgcacc ggcgacaaga acaatcagta caaaattacc ctcgcacaac
    13381 aatggcaggc tggcgacagc atctggtcac gcccggctat tcgtgtcttc gcaacctacg
    13441 ccaagtggga tgagaaatgg ggttatgact acaacggcga tagcaaggtt aacccgaact
    13501 acggcaaagc cgttcctgct gatttcaacg gcggcagctt cggtcgtggc gacagcgacg
    13561 agtggacctt cggtgcccag atggaaatct ggtggtaata gcaaaacctg ggccggataa
    13621 ggcgtttacg ccgcattcga caaccaacgc ctgatgcgac gcttgcgcgt cttatcaggc
    13681 ctacaacggc tgtcaaatgt aggccggata aggcgtttac gccgcatccg gcataaaaac
    13741 aggttgtcat tatctgaaag gggcgaaagc ccctctgatt atcgggttta gcgcgctatt
    13801 gcctggctac cgctgagctc cagattttga ggtgaaaaca atgaaaatga ataaaagtct
    13861 catcgccctc tgtttatcag cagggttact ggcaagcgcg cctggaatca gccttgccga
    13921 tgttaactac gtaccgcaaa acaccagcga cgcgccagcc attccatctg ctgcgctgca
    13981 acaactcacc tggacaccgg tcgatcaatc taaaacccag accacccaac tggcgaccgg
    14041 cggccaacaa ctgaacgttc ccggcatcag tggtccggtt gctgcgtaca gcgtcccggc
    14101 aaacattggc gaactgaccc tgacgctgac cagcgaagtg aacaaacaaa ccagcgtttt
    14161 tgcgccgaac gtgctgattc ttgatcagaa catgacccca tcagccttct tccccagcag
    14221 ttatttcacc taccaggaac caggcgtgat gagtgcagat cggctggaag gcgttatgcg
    14281 cctgacaccg gcgttggggc agcaaaaact ttatgttctg gtctttacca cggaaaaaga
    14341 tctccagcag acgacccaac tgctcgaccc ggctaaagcc tatgccaagg gcgtcggtaa
    14401 ctcgatcccg gatatccccg atccggttgc tcgtcatacc accgatggct tactgaaact
    14461 gaaagtgaaa acgaactcca gctccagcgt gttggtagga cctttatttg gttcttccgc
    14521 tccagctccg gttacggtag gtaacacggc ggcaccagct gtggctgcac ccgctccggc
    14581 accggtgaag aaaagcgagc cgatgctcaa cgacacggaa agttatttta ataccgcgat
    14641 caaaaacgct gtcgcgaaag gtgatgttga taaggcgtta aaactgcttg atgaagctga
    14701 acgcctggga tcgacatctg cccgttccac ctttatcagc agtgtaaaag gcaaggggta
    14761 attacgcccc acagtgctga ttttgcaaca actggtgcgt ctcctggcgc accttttttt
    14821 atgcttcctt cctgggatat gagcgatttt ttatagtaac tcacttcttc ttcactaaga
    14881 atatccatta tctcaatgcc ttatcagaga ttcttttcct ttcgccggta gtgtctggac
    14941 attcaggcta cttttccagg ttattttatt tctgttatgc agaggtttta tgataagtca
    15001 tatcctaaat tctggcggca ataactcttt gatgaaacat gatgtggtgc aaggaaataa
    15061 tatagtagat cttgatttac tacgtaattt caatggggtg ccaggtttaa acagagataa
    15121 ctttatttat atcagcaata ttttttcaaa tataaaacaa cggaacgaaa aaatcatgca
    15181 ataaatatgt ttcgtgaagt ctcaatcagt aatgatacta taagtgtaaa attctacaga
    15241 aatgaaaaaa attgaatgcg cttgcaattt tctgatggat aaagatgcgc aggggtatat
    15301 cgacctgtct gatttggatt taacaagttg tcattttaaa ggtgacgtta tatcgaaggt
    15361 gtctttttta tcatcaaatc tacaacatgt aacattcgaa tgtaaagaaa ttggggattg
    15421 caattttact actgcaatag ttgataatgt catatttaga tgtcgacgtt tacacaatgt
    15481 gatttttatc aaagcgagtg gtgaatgtgt cgatttcagc aaaaatattc ttgatacagt
    15541 tgacttctcg cagagtcaac ttactcagag taattttcgc gaatatcaga ttagaaattc
    15601 aaacttcgat aattgttatc tttacgcttc gcacttcacc agagcagagt ttctgtctgc
    15661 caaagaaata tcatttatta aatcgaatat gacagctgtt atgtttgatc atgtgcgaat
    15721 atcgacaggg aattttaaag attgcattac agaacaattg gaattaact
//
LOCUS       DAJOWB010000108        15669 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJOWB010000108 DAJOWB010000000
VERSION     DAJOWB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.108"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..880
                     /locus_tag="OHO85_003676"
     CDS             <1..880
                     /locus_tag="OHO85_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2672996.1"
                     /translation="HITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQL
                     GVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNYI
                     LPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLED
                     ELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMELG
                     NSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLSN
                     ALRRFLDYCDPANVPR"
     gene            1085..2554
                     /gene="lysP"
                     /locus_tag="OHO85_003677"
     CDS             1085..2554
                     /gene="lysP"
                     /locus_tag="OHO85_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCQ2672997.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2848..4840
                     /gene="cirA"
                     /locus_tag="OHO85_003678"
                     /pseudo
     CDS             2848..4840
                     /gene="cirA"
                     /locus_tag="OHO85_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(4872..5708)
                     /gene="yeiG"
                     /locus_tag="OHO85_003679"
     CDS             complement(4872..5708)
                     /gene="yeiG"
                     /locus_tag="OHO85_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCQ2672998.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5966..6634
                     /gene="folE"
                     /locus_tag="OHO85_003680"
     CDS             5966..6634
                     /gene="folE"
                     /locus_tag="OHO85_003680"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCQ2672999.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6651..7808
                     /locus_tag="OHO85_003681"
     CDS             6651..7808
                     /locus_tag="OHO85_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCQ2673000.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLVGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            7950..8990
                     /gene="galS"
                     /locus_tag="OHO85_003682"
     CDS             7950..8990
                     /gene="galS"
                     /locus_tag="OHO85_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCQ2673001.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            9270..10268
                     /gene="mglB"
                     /locus_tag="OHO85_003683"
     CDS             9270..10268
                     /gene="mglB"
                     /locus_tag="OHO85_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCQ2673002.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10329..11849
                     /gene="mglA"
                     /locus_tag="OHO85_003684"
     CDS             10329..11849
                     /gene="mglA"
                     /locus_tag="OHO85_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCQ2673003.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11865..12875
                     /gene="mglC"
                     /locus_tag="OHO85_003685"
     CDS             11865..12875
                     /gene="mglC"
                     /locus_tag="OHO85_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCQ2673004.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(12946..14181)
                     /gene="preA"
                     /locus_tag="OHO85_003686"
     CDS             complement(12946..14181)
                     /gene="preA"
                     /locus_tag="OHO85_003686"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCQ2673005.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKNRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(14175..15413)
                     /gene="preT"
                     /locus_tag="OHO85_003687"
     CDS             complement(14175..15413)
                     /gene="preT"
                     /locus_tag="OHO85_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCQ2673006.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKMLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSNVEIAVDFLQRARQAQGDISIPQSALIIGCGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
BASE COUNT         3720 a         3860 c         4112 g         3977 t
ORIGIN      
        1 gcacatcacc ctccggcagt tggaagtttt tgcagaagta ttgaaaagtg gatcaaccac
       61 ccaggcgtcg gtgatgctgg cgttgtcgca atcagcagtg agcgcagcct tgaccgacct
      121 ggaagggcag cttggcgttc aactgtttga tcgcgtgggg aaaagactgg ttgttaatga
      181 acacgggcgg ctgctctatc cgcgagcgtt ggcactgctt gaacaggcgg ttgaaatcga
      241 acaactgttt cgcgaagaca acggcgcgat tcgtatctat gccagtagta ccatcggtaa
      301 ctacattctg cctgcagtta tcgcccgtta tcgccatgat tatccgcagt tgccgattga
      361 acttagcgtt gggaatagcc aggatgtgat gcaagcggtg ctggatttcc gcgttgatat
      421 tggctttatt gaaggaccgt gccacagcac tgaaatcatt tctgaaccgt ggctggaaga
      481 cgaactggtg gttttcgccg cgccgacttc gccgttggcc cgtggtccgg tcaccttaga
      541 gcagctggcc gctgcgccgt ggatcctgcg tgaacgcggt tccggcacgc gggagattgt
      601 cgattatcta ttgctgtcac atttaccgaa gtttgagatg gcgatggaat taggtaactc
      661 cgaggcaatc aaacatgcgg tgcgtcatgg gttgggaatt agttgcctgt cgcgacgtgt
      721 gattgaagat caattgcagg caggcacatt aagtgaagtt gcggtccctc tcccgcgcct
      781 gatgcgtacg ttgtggcgta tacatcatcg gcaaaaacac ctttccaacg cgctacggcg
      841 ctttctggac tattgcgatc ccgcgaatgt accgcgttaa ggtgctgtac aagaacatgc
      901 tggtgctgtg tcgatttcgt gacgcagcgc cctcagcatg cattcgccag aaaagagatt
      961 ggctgcttta cttataatcc ctgggcgatc atgaaggtgt cttataaccg tgtatttctg
     1021 ccggaaggat tgccaatcgt ctgctacaat cgcgcctcat ttttaagatg gatagcattt
     1081 ttgtatggtt tccgaaacta aaaccacaga agcgccgggc ttacgccgtg aattaaaggc
     1141 gcgtcacctg acgatgattg ccattggcgg ttccatcggt acaggtcttt ttgttgcctc
     1201 tggcgcaacg atttctcagg caggtccggg cggggcattg ctctcgtata tgctgattgg
     1261 cctgatggtt tacttcctga tgaccagtct cggtgaactg gctgcatata tgccggtttc
     1321 cggttcgttt gccacttacg gtcagaacta tgttgaagaa ggctttggct tcgcgctggg
     1381 ctggaactac tggtacaact gggcggtgac tatcgccgtt gacctggttg cagctcagct
     1441 ggtcatgagc tggtggttcc cggatacacc gggctggatc tggagtgcgt tgttcctcgg
     1501 cgttatcttc ctgctgaact acatctcagt tcgtggcttt ggtgaagcgg aatactggtt
     1561 ctcactgatc aaagtcacga cagttattgt ctttatcatc gttggcgtgc tgatgattat
     1621 cggtatcttc aaaggcgcgc agcctgcggg ctggagcaac tggacaatcg gcgaagcgcc
     1681 gtttgctggt ggttttgcgg cgatgatcgg cgtagctatg attgtcggct tctctttcca
     1741 gggaaccgag ctgatcggta ttgctgcagg cgagtccgaa gatccggcga aaaacattcc
     1801 acgcgcggta cgtcaggtgt tctggcgaat cctgttgttc tatgtgttcg cgatcctgat
     1861 tatcagcctg attattccgt acaccgatcc gagcctgctg cgtaacgatg ttaaagacat
     1921 cagcgttagt ccgttcaccc tggtgttcca gcacgcgggt ctgctctctg cggcggcggt
     1981 gatgaacgca gttattctga cggcggtgct gtcagccggt aactccggta tgtatgcgtc
     2041 tactcgtatg ctgtacaccc tggcgtgtga cggtaaagcg ccgcgcattt tcgctaaact
     2101 gtcgcgtggt ggcgtgccgc gtaatgcgct gtatgcgacg acggtgattg ccggtctgtg
     2161 cttcctgacc tccatgtttg gcaaccagac ggtatacctg tggctgctga acacctccgg
     2221 gatgacgggt tttatcgcct ggctggggat tgccattagc cactatcgct tccgtcgcgg
     2281 ttacgtattg cagggacacg acattaacga tctgccgtac cgttcaggtt tcttcccact
     2341 ggggccgatc ttcgcattca ttctgtgtct gattatcact ttgggccaga actacgaagc
     2401 gttcctgaaa gataccattg actggggcgg cgtagcggca acgtatattg gtatcccgct
     2461 gttcctgatt atttggttcg gctacaagct gattaaagga actcacttcg tacgctacag
     2521 cgaaatgaag ttcccgcaga acgataagaa ataagtttcc tcccttcctt gctaagccct
     2581 ctcaaccgag agggcttttt caattccatt tccctgacaa atcatgcgga tataaaattt
     2641 aacatttgga ttgataattg ttatcgtttg cattatcgtt acgccacaat caaaaaaggc
     2701 tgacaaatca gaggctgttc cggctttctg ggatgatcac ctgcataaaa aataagtcca
     2761 ccgcgatgct gccgtacgca aggggacgtg aagaagatgt gagcgataac ccattttatt
     2821 ttcgtagtta cctcatggag atatggaatg tttaggttga accctttcgt acgggtcggg
     2881 ctgtgtttgt ccgctatttc ttgtgcatgg cctgtgttag cggtcgatga tgatggcgaa
     2941 acgatggttg tcactgcatc ttccgtggaa caaaatctta aagatgcacc agccagtatc
     3001 agcgtcatta cccaggaaga cctgcagcga aaaccggtac agaatctgaa ggatgtcctc
     3061 aaaagaagtg cctggcgtac aactgacgaa cgaaggggat aaccgtaagg gcgttagtat
     3121 tcgtggtctg gacagcagct acaccctgat tcttgtcgac ggtaaacgcg ttaactcccg
     3181 caatgccgtc ttccgccaca atgatttcga tctgaactgg atcccggtcg attccatcga
     3241 acgtattgaa gtggttcgtg gcccgatgtc gtcgctgtac ggctccgatg cgctcggcgg
     3301 tgtagtgaat atcatcacca aaaaaatcgg tcagaaatgg tcgggtaccg ttaccgtcga
     3361 taccaccatt caggaacatc gcgatcgcgg tgatacctat aacggtcagt tctttaccag
     3421 cggaccatta attgacggtg tgctgggaat gaaagcttac ggcagcttgg caaaacgtga
     3481 aaaggatgac ccgcaaaact caacgaccac cgataccgga gaaacgccgc gtattgaagg
     3541 attctccagc cgcgacggta atgtcgaatt tgcctggaca ccgaatcaaa atcacgattt
     3601 tactgccgga tacggtttcg accgtcagga tcgtgattcc gactcgctgg acaaaaaccg
     3661 cctggaacgc cagaactact ccgtcagcca taatgggcgt tgggattacg gcaccagcga
     3721 actgaaatac tacggtgaga aagtcgagaa caaaaaccct ggcaacagca gcccgataac
     3781 ttccgaaagc aatacggtcg acggcaaata cacgttgccg ctgacggcga ttaatcagtt
     3841 tctcacggtt ggcggtgaat ggcgtcacga caaacttagc gatgcggtga acctgaccgg
     3901 gggaaccagc tccaaaacgt ctgccagcca gtacgcgctg tttgtggaag atgaatggcg
     3961 gatcttcgag ccgctggcgc tgacgaccgg cgtgcgtatg gacgatcacg aaacctacgg
     4021 tgaacactgg agtccgcgtg cctacctggt ttataacgcc accgacaccg taacggtgaa
     4081 agggggctgg gcgacggcat ttaaagcacc ttctctgttg caacttagcc ctgactggac
     4141 gagcaattcc tgccgtggcg catgtaagat tgtgggtagc ccggatctga aaccagaaac
     4201 cagcgaaagt tgggagctgg ggctttacta catgggtgaa gaaggctggc tggaaggggt
     4261 tgaatccagc gttaccgttt tccgtaacga tgtgaaagat cgtatcagca tcagccgtac
     4321 gtctgacgtc aacgctgcac cgggctacca aaactttgtc ggttttgaga cgggcgctaa
     4381 cggacggcgc ataccggtat ttagctacta caacgttaac aaagctcgta ttcagggcgt
     4441 ggaaaccgaa ctgaaaattc cgttcaacga tgaatggaaa ctgtcattca actacaccta
     4501 caacgatggt cgtgatgtca gcaacggcga aaacaaaccg ctatccgatc tgccgttcca
     4561 taccgctaac ggtacgctgg actggaaacc gctggcgctg gaagactggt cattctatgt
     4621 ttctggtcac tataccgggc agaaacgagc cgacagcgcg acggctaaaa cacctggcgg
     4681 ttataccatc tggaataccg gcgcggcctg gcaggtgact aaagacgtca aactgcgcgc
     4741 aggcgtgctg aaccttggcg acaaggatct cagccgtgat gactacagct ataacgaaga
     4801 cggacgtcgt tactttatgg cagtggatta tcgcttctga tgagaagatg cccggcgaac
     4861 cgggcggact ttcacttcag taaatactgc gcatggaagc gcaggtgatc ctctataaaa
     4921 gaggcgatga agtagtaact gtgatcatat cccggctgaa tacgcagcgt catcggccac
     4981 gctttctggc gcgcggcttc tgccagaact gcgggttgca actgatcggc aagaaactga
     5041 tcattatcac cttgatcgat aagcgtcggg atcgcatcct gcgcgttact ggcatacatc
     5101 agcgcgcaac tgtcccattc cagccatgca tttttgtcct cacctaaata gcggctaaac
     5161 gctttgatcc cccacgggac gctgcacgga ttcacaatgg gcgcaaaggc ggaaacgctg
     5221 gtgtatttgc ccgggttttt cagcgccata atcagcgcac cgtgaccacc cattgaatga
     5281 ccgctaatgg cgcagcggtc gctgacatta aattgcgact gaaccagcgc cggtaattca
     5341 tcgcgcagat aatcatacat ccggtaatgc gtcgcccacg gcggttgcgt ggcattaaga
     5401 taaaagcctg cgccctggcc taaatcgtag ccatcgtcgt tggcaacctg ttcgccgcgc
     5461 gggctggtgt ctggcatcac cagtacaatc cccagttccg ccgctacccg ctgggcaccc
     5521 gccttggtgg tgaagttctc gtcattgcag gttaatccgg aaagccagta cagcactggt
     5581 ggtggggtgt gatcacgagg tggtgggaga aagatactga acgtcatcgg gcagtttaag
     5641 gtactggagt cgtgtcgcca gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc
     5701 atttccatgc gtggctcctt gttgtgttgt ttgcaatttc ttcatcatac cgtttattca
     5761 atgtgctgtg agtaactttc acttccgtat ttgcataacg atgttttaac atctgctgat
     5821 gaaaggcagc ggcaattaca ataattatcg ctgtgaatac tggattatgt gcgccgcctc
     5881 acgcacaata atcaggctgt aaatcagctt aataactttg cccccacgca gggcggaggc
     5941 gtcacacctg caggagaaat cataaatgcc atcactcagt aaagaagcgg ccctggttca
     6001 tgaagcgtta gttgcgcgag gactggaaac accgctgcgc ccgcccgtgc atgaaatgga
     6061 taacgaaacg cgcaaaagcc ttattgctgg tcatatgacc gaaatcatgc agctgctgaa
     6121 tctcgacctg gctgatgaca gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt
     6181 cgatgaaatt ttctccggtc tggattacgc caacttcccg aaaatcaccc tcattgaaaa
     6241 caaaatgaag gtcgatgaaa tggtcaccgt gcgcgatatc actctgacca gtacctgtga
     6301 acaccatttt gttaccatcg atggcaaagc gacggtggct tatatcccga aagattcggt
     6361 gatcggtctg tcaaaaatta accgtatcgt gcagttcttt gcccagcgtc cgcaggtgca
     6421 ggaacgtctg acgcagcaaa ttcttattgc gctacaaacg ctgctgggca ccaataacgt
     6481 ggctgtctcg atcgacgcgg tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac
     6541 cagtgccacg acaacgacct ctcttggtgg attgttcaaa tccagtcaga atacgcgcca
     6601 cgagtttctg cgcgctgtgc gtcatcacaa ctgattaaaa ggcaggaacc atggagcgca
     6661 acgtcacgct cgattttgtt cgcggcgtcg ccattctggg gatcctgcta ttaaacatca
     6721 gcgcctttgg gctaccaaag gcggcttatc tcaatcccgc ctggtacggc gctattacgc
     6781 cgcaggatgc atggacctgg gcatttcttg atctcgtcgg ccaggtgaaa ttcctcacgc
     6841 tttttgcgct gctgtttggt gcgggcctgc aaatgttgct gccccgtggc agacgctgga
     6901 tccagtcgcg gttaacgctg ttagtcttgc tgggttttat tcacggttta ttgttctggg
     6961 acggcgatat tctgctggct tacgggctgg tgggcttaat ctgctggcgg ctggtgcgcg
     7021 atgcgccatc ggtaaaaagc ctgtttaata caggcgtcat gctttatctg gtggggcttg
     7081 gcgttttgct gttattgggg ctgatttccg acagccagac cagccgcgcc tggacgccgg
     7141 atgcatcggc tattttgtat gaaaaatact ggaagcttca cggcggcgtt gaagcgatca
     7201 gtaatcgtgc cgatggtgtt ggcaacagtt tactggcact gggcgcacag tatggctggc
     7261 aactggcagg gatgatgctc attggtgcgg cgctgatgcg cagcggctgg ctgaaagggc
     7321 agttcagctt acgtcactat cgtcgtactg gttttgtgct ggtggcgatt ggggtgacca
     7381 ttaaccttcc tgccatcgcc ctgcaatggc agctggactg ggcgtatcgc tggtgtgcct
     7441 tcttacttca aatgccgcgg gaactgagtg cgccgtttca ggcgattggc tatgcgtcgc
     7501 tgttttatgg cttctggccg caattgagcc gctttaagct ggtgcttgcg atcgcctgcg
     7561 tcggacggat ggcgctgacc aactatctat tgcaaacgct gatttgtacc atgctttttt
     7621 accacctcgg tttgtttatg cattttgatc gcctggagct gctggcgttt gttattccgg
     7681 tatggctggc gaatatcctc ttctctgtta tctggctgcg ttacttccgc caggggccgg
     7741 tggaatggct ctggcgtcag ttaactttgc gtgctgccgg aacggcaata tctaaaacat
     7801 caagataacg atctggatca cattcgttaa caaaacggct gtaaccgttt ccattgctgt
     7861 gactcgattc acgaagtcct gtattcagtg ctgacaaaat agccgccagc aagcagtcat
     7921 ttactgcaat ctcataacag gtagtgaata tgatcaccat tcgtgatgta gcgcgtcagg
     7981 ctggcgtctc tgtggcaacg gtttcccggg tgctcaataa cagcacgctg gtcagtgccg
     8041 acacgcgtga agcagtaatg aaagccgtga gtgagctgga ttatcggcca aatgccaatg
     8101 cccaggcgct ggcaactcag gttagcgata ccattggcgt ggtggtgatg gacgtttctg
     8161 atgctttttt cggcgcgctg gtaaaagcgg tggatctggt cgcccagcag catcagaaat
     8221 acgtgctaat cggcaatagc tatcatgaag cggaaaaaga gcgtcacgcc attgaggtgt
     8281 taattcgcca gcgttgtaat gcgttgattg ttcactcaaa agcattgagt gacgatgaac
     8341 tggcgcaatt tatggataac attcccggta tggtgttaat caaccgcgtt gtgccggggt
     8401 acgcccatcg ttgcgtttgc ctggataatc tcagcggtgc ccgaatggcg acgcgcatgt
     8461 tgctgaataa cggtcatcaa cgtattggtt atctttcttc cagccacggc attgaagatg
     8521 acgccatgcg taaagcaggc tggatgagtg cgttgaaaga gcaggatatt attccgccgg
     8581 aaagctggat tggcactggt acgccggaca tgccgggcgg tgaggcggcg atggttgaac
     8641 tgctggggcg caatctacaa cttaccgctg tatttgctta taacgacaat atggccgctg
     8701 gcgcactgac agcattaaaa gataatggca ttgcgattcc gttacatctc tcaatcatcg
     8761 gtttcgatga tattcccatc gcccgttaca ccgacccgca attaacgacc gtgcgttatc
     8821 ccattgcttc aatggctaaa ttagccaccg aactggcctt gcagggggca gcaggcaata
     8881 ttgatcctcg tgccagccac tgttttatgc cgacgttagt gcgtcgtcat tctgtcgcaa
     8941 cgcgccagaa tgcggcggcg atcactaact caacaaatca ggcgatgtaa ccgctttcaa
     9001 tctgtgagtg atttcacagt atcttaacaa tgtgatagct atgattgcac cgttttaacg
     9061 ttgtaacccg tatgtaacag tgaataatca ctttcaccga ggtaatagcg tcataacaac
     9121 aattaaagcc gttttctgga gcgttaccgg gcatggaaga acgaatttta aaaagtgagc
     9181 ttcggcgttc agtaacactt cattaactct actgccccgc cgagcattta tctcaagcac
     9241 taccctgcat aagaaaaacc ggagatacca tgaataagaa ggtgttaacc ctgtctgctg
     9301 tgatggccag catgttattc ggtgccgctg cacacgctgc tgatactcgc attggtgtaa
     9361 caatctataa gtacgacgat aactttatgt ctgtagtgcg caaggctatt gagcaagatg
     9421 cgaaagccgc gccagatgtt cagctgctga tgaatgattc tcagaatgac cagtccaagc
     9481 agaacgatca gatcgacgta ttgctggcga aaggggtgaa ggcactggca atcaacctgg
     9541 ttgacccggc agctgcgggt acggtgattg agaaagcgcg tgggcaaaac gtgccggtgg
     9601 ttttcttcaa caaagaaccg tctcgtaagg cgctggatag ctacgacaaa gcctactacg
     9661 ttggcactga ttccaaagag tccggcatta ttcagggcga tttgattgct aaacactggg
     9721 cggcgaatca gggttgggat ctgaacaaag acggtcagat tcaattcgta ttgctgaaag
     9781 gtgaaccggg ccatccggat gcagaagcac gtaccactta cgtgattaaa gagctgaacg
     9841 acaaaggcat taaaactgaa cagttacagt tagataccgc tatgtgggat accgctcagg
     9901 cgaaagataa gatggacgcc tggctgtctg gcccgaacgc caacaaaatc gaagtggtta
     9961 tcgccaacaa cgatgcgatg gcaatgggcg cggtagaagc actgaaagca cacaacaagt
    10021 ccagcattcc ggtgtttggc gtcgatgcgc tgccagaagc gctggcgctg gtgaaatccg
    10081 gtgcactggc gggcacagta ctgaacgatg ctaacaacca ggcgaaagcg acctttgatc
    10141 tggcgaaaaa cctggccgat ggcaaaggtg cggctgatgg caccaactgg aaaatcgaca
    10201 acaaagtggt ccgcgtacct tatgttggcg tagataaaga caacctggct gaattcagca
    10261 agaaataaga ctgattgggc gcagtcgttt actgcgccct tattgtatgg ccaacaaggt
    10321 aaaattatat ggtcagctca acgactccgt cttccgggga atacttgttg gaaatgagcg
    10381 gtatcaacaa gtcctttcct ggtgttaagg cacttgataa cgttaattta aaagtccggc
    10441 cacattctat ccatgcatta atgggggaaa acggtgcagg aaaatcgaca ttattaaaat
    10501 gcctgtttgg tatttatcaa aaagactccg gcaccatttt attccagggt aaagagatcg
    10561 atttccattc cgccaaagag gcactggaaa atggtatttc gatggtacac caggagttaa
    10621 acctggtatt acaacgttcg gtgatggata acatgtggct ggggcgatat cccaccaaag
    10681 gcatgtttgt cgatcaggac aaaatgtacc gcgaaaccaa agcgattttt gatgaactgg
    10741 atattgatat cgatccgcgt gcgcgcgtcg gcacattatc cgtttcgcaa atgcagatga
    10801 tcgaaatcgc caaagcgttt tcctataacg cgaaaattgt gattatggat gaaccgactt
    10861 cttcgttaac cgaaaaagag gtcaatcatc tgttcactat tattcgtaaa ttaaaagagc
    10921 gcggctgcgg tattgtttat atctcgcata aaatggaaga aatcttccag ttatgtgatg
    10981 aagttactgt attgcgcgac ggtcagtgga tcgccaccga accgctggca ggactgacga
    11041 tggacaagat catcgccatg atggttgggc gttctcttaa tcagcgtttt cctgacaaag
    11101 aaaacaagcc gggcgaagtc atcctcgagg tgcgtaacct aacgtcactg cgccagccgt
    11161 cgattcgcga tgtctcgttt gatctgcata aaggggagat cctcggtatt gccgggctgg
    11221 tgggggcgaa acgtaccgat attgttgaga cgttatttgg tattcgcgag aaatcggctg
    11281 gcaccattac gttgcacggc aaacagatca ataaccataa tgccaacgaa gccataaacc
    11341 acggatttgc actggtaact gaggagcgcc gctcaacggg aatttatgcc tatctggata
    11401 ttggttttaa ctcgttaatt tccaatattc gcaactacaa aaataaagtt ggtttactgg
    11461 ataactcgcg gatgaaaagc gatacccagt gggtgattga ttcgatgcgg gtaaaaacgc
    11521 cgggtcatcg gacgcaaatt ggttcgctct ccggtggtaa tcagcaaaag gtgattattg
    11581 gtcgctggct actaacgcaa ccagaaatat taatgctcga tgaaccgacg cgcggtattg
    11641 atgtcggggc gaagtttgaa atttatcagt taattgccga actggcgaag aaaggcaagg
    11701 ggattattat tatctcctct gaaatgcctg agttgttagg gataacagac cgtattctgg
    11761 tcatgagcaa tggtctcgtt tccggaattg tcgatacaaa aacaacaacg caaaacgaaa
    11821 ttctgcgtct tgcgtctttg cacctttaag attaggggct tcccatgagt gcgttaaata
    11881 agaaaagttt tcttacttac ctgaaagagg gcggtattta cgtcgttctt ttagttttgc
    11941 tggcgattat tattttccag gacccaacat ttttaagtct gttgaactta agtaatattc
    12001 tcacccagtc atcggtgcgt attattatcg cgctcggtgt ggcagggtta attgtcaccc
    12061 aggggaccga tctttctgct ggtcgtcagg tagggctggc ggcagtggtg gctgcgacat
    12121 tattgcagtc catggataac gccaacaaag tgttcccgga aatggcgacg atgccgattg
    12181 cgctggttat tctgattgtc tgtgccattg gtgcggtgat cggtttgatc aacggtctga
    12241 ttatcgctta tctcaacgtg acgccgttca ttaccacgct cggcacgatg atcatcgtct
    12301 atggcatcaa ctcgctctat tacgactttg tcggggcgtc gccaatttct ggttttgaca
    12361 gtggcttctc tacctttgct cagggctttg tcgcgctggg gagtttccgt ctctcttaca
    12421 tcaccttcta cgcgttgatt gcggtggcgt tcgtctgggt gttgtggaac aaaacccgct
    12481 tcggtaagaa catttttgcc attggcggta acccggaagc ggcaaaagta tctggtgtca
    12541 acgtcggcct gaacctgctg atgatctacg cgttgtctgg cgtgttctat gcctttggcg
    12601 ggatgttaga agccggacgt atcggctctg ccaccaacaa cctcggcttt atgtatgagc
    12661 tggatgctat cgcggcgtgc gtggtaggcg gtgtatcgtt cagcggcggt gtggggacgg
    12721 tgattggcgt ggtgaccggg gtaattattt ttaccgtcat caactatggc ctgacgtata
    12781 tcggcgtaaa cccatactgg cagtacatca tcaaaggggc gattattatc ttcgccgtag
    12841 cgctggattc actgaaatac gcgcgtaaga agtgatgtga ttgtggatat tgccggatgc
    12901 gctgcatccg gcaatcggtg ccaaatgccg gatgcgtctc tggttttaca acgttaccgg
    12961 gtgttctttc tcgcctttct taaacttcac ttccccgagc tcaatacaac ccaccgggca
    13021 gacgtgacca caaagcagac aacccacaca tttctcggta ttacaatgcg gtgtgcggtt
    13081 tttctcgctc cattccatcg cctggtgacc gccgtcgtaa caggaaatat aacagcgtcc
    13141 acagccaaca catttatcaa gattgatacg gggatagaca atataactgc ggtctaaatc
    13201 ttccgccggg acaatattgt tattcgccag acctaccatc tcctgcagcg aatcaaatcc
    13261 ctgatcggcg agataatgcg acaacccgct tgccatatct tccactatcc gatacccgta
    13321 ctgcatgatg ccggtggtca cctgtaacgt tgctgcgccg agcaataaaa actcagccgc
    13381 atcctcccag gtttcaatgc cgccgatacc gctgattggg aaatcgcgca gttctggatg
    13441 ggtgcgcatt tgctgaatga agcgcagggc gatcggtttt accgctttac cggaatatcc
    13501 ggaaatactc gattttccgt taacgatcgg cataccgatt ttctgattaa gatcgatatt
    13561 ggtgatggat ttaacggtgt taatcgccgc aatgccatct gcgccgccgc gcttcgccgc
    13621 cagcgccact tcgcacatat caccgatatt cggcgtcatc ttcgccagca ttggcagcgt
    13681 ggaaccccgt ttcactgccc gacaatattt ttctaccagc tccgggcttt gcccgacatc
    13741 gctacccatc gcatgagaag tcatttgcgg acaggagaag ttacactcga tcatatccgc
    13801 gccagcttct tgcaccaggc gcgccagctc ctcccattgc tgctcatttt cccccatgat
    13861 cgaagcgatc aatactttgt ccgggtaatc ttccttcagc tgacgcaggg cggccagatt
    13921 ctcttccaac ggatgttcag caatctgctc catatttttg aagccgataa aaccggtatc
    13981 ttctttcacc agatgatcaa aacgcggcga gacttcgttg gcgataaaaa agccgatcgt
    14041 tttaaacacc acgccgcccc aacctgtgtc gtaggctttg gcacacatct catagcagtt
    14101 gcctacaggc gaagaagaga ggcagaacgg gttgggaaac ttcacgccgc aaaaagtaat
    14161 cgaaagatct ttcgttaaca tgagcaagct ccctctaaat agtgatgaat cgcctcggcg
    14221 gcttctttcc cggttttcac ggcatagacc acggttttgt caccctcaac aatatcgcca
    14281 gcagcaaaga cttgcggatc gcgggtctga taattttgtg ttttgatggt gttacgctgc
    14341 ggctctaact ccgcaaaggc atccagtctg gcatgctgac cgacggcgag aataatttta
    14401 tctgccgcca tcgtcagttc gcccggtaac cgtacatgct taaacgtgac tttattgcct
    14461 tccacggcta ctggcgtgaa tccatcgatg atcgaaacgc ccagctcccg ggcgctggca
    14521 aactcttttt cgcttgccgg aaactcatct aactcttcac gcgctacgca ggttaccgcc
    14581 tgacagccga gaactttcag cgtgctggct acgtccatcg caacatcacc gcagccgata
    14641 attaatgcgc tttgtggaat gctgatatcg ccttgcgcct gtcgtgcacg ttgcaggaag
    14701 tccacggcaa tctcaacgtt gctatgctca aacagcgata acccggaacc gctcgataac
    14761 cccacggtga ccagtaccgc gcggttttct gctttcagct gctcaagggt gagtgtgtta
    14821 ccgacttcgt tgttgcactt aatggtcacg cccatttttt caatacgggc gatctctgca
    14881 tccagcactg attgcggtaa ccggaattgc ggaataccgt tacgcaacca gccaccgggg
    14941 tgcgcttctt tctcataaat cgtgacgtca taaccctggt ttgtcagtgt tacactggcc
    15001 tgcaatccgg caggacctgc gccaataatc gcgactttgc cgagcatttt agtaccgggc
    15061 tgataaattt ccatcccagt ttgttgttca aaatcagtaa caaaacgctg taagcgaccg
    15121 atatcaatgg gagtatcaac accggcacgg gtacaaccgc tttgacataa tttctccgtc
    15181 gggcacactc tggcacaaac ggcaccgagg gcattatttt cgcgaattgt ctcggcagcg
    15241 cctttaaaat tacgaaagta gattgagcga ataaatttcc ccggatcggt ctgtgccggg
    15301 caagcctgac tacagggagc gtcatgacat aataaacagc gagaggcttc tttaatcgcc
    15361 agtaaaggcg taaaagccgg agtgagttca tccagataat tttgttgcgg catgtgatct
    15421 tccttaatgc tatgagtaat taaggcgaac gctaaatata ctcgtcatac ttcaagttgc
    15481 atgtgctgcg tctgcgttcg ctcaccccag tcacttactt atgtaagctc ctggggactc
    15541 actcacttgt cgccttcctg caactcgaat tatttagagt ataaatattg cgtgctgata
    15601 ttaaggctat gcgcgtgggg aaatttatac tgtgaaagga ttcacaaata gctgcggttc
    15661 agaatgtta
//
LOCUS       DAJOWB010000109        15666 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJOWB010000109 DAJOWB010000000
VERSION     DAJOWB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.109"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(238..1350)
                     /gene="ydiK"
                     /locus_tag="OHO85_003688"
     CDS             complement(238..1350)
                     /gene="ydiK"
                     /locus_tag="OHO85_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCQ2673007.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1739..4795
                     /locus_tag="OHO85_003689"
     CDS             1739..4795
                     /locus_tag="OHO85_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCQ2673008.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4792..5202
                     /gene="menI"
                     /locus_tag="OHO85_003690"
     CDS             4792..5202
                     /gene="menI"
                     /locus_tag="OHO85_003690"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCQ2673009.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5302..5490
                     /locus_tag="OHO85_003691"
     CDS             5302..5490
                     /locus_tag="OHO85_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCQ2673010.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            6038..6406
                     /gene="sufA"
                     /locus_tag="OHO85_003692"
     CDS             6038..6406
                     /gene="sufA"
                     /locus_tag="OHO85_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCQ2673011.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6415..7902
                     /gene="sufB"
                     /locus_tag="OHO85_003693"
     CDS             6415..7902
                     /gene="sufB"
                     /locus_tag="OHO85_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCQ2673012.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7912..8658
                     /gene="sufC"
                     /locus_tag="OHO85_003694"
     CDS             7912..8658
                     /gene="sufC"
                     /locus_tag="OHO85_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCQ2673013.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIYMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8633..9904
                     /gene="sufD"
                     /locus_tag="OHO85_003695"
     CDS             8633..9904
                     /gene="sufD"
                     /locus_tag="OHO85_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCQ2673014.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9901..11121
                     /gene="sufS"
                     /locus_tag="OHO85_003696"
     CDS             9901..11121
                     /gene="sufS"
                     /locus_tag="OHO85_003696"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCQ2673015.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDSEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAPAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPRNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            11134..11550
                     /gene="sufE"
                     /locus_tag="OHO85_003697"
     CDS             11134..11550
                     /gene="sufE"
                     /locus_tag="OHO85_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCQ2673016.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11699..12703
                     /gene="ldtE"
                     /locus_tag="OHO85_003698"
     CDS             11699..12703
                     /gene="ldtE"
                     /locus_tag="OHO85_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCQ2673017.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12767..13003)
                     /gene="lpp"
                     /locus_tag="OHO85_003699"
     CDS             complement(12767..13003)
                     /gene="lpp"
                     /locus_tag="OHO85_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCQ2673018.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(13314..14726)
                     /gene="pykF"
                     /locus_tag="OHO85_003700"
     CDS             complement(13314..14726)
                     /gene="pykF"
                     /locus_tag="OHO85_003700"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCQ2673019.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            15283..15492
                     /gene="fumD"
                     /locus_tag="OHO85_003701"
     CDS             15283..15492
                     /gene="fumD"
                     /locus_tag="OHO85_003701"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCQ2673020.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         3966 a         3788 c         4129 g         3783 t
ORIGIN      
        1 ttcactcagg ggatttccat gcttataaat caatatgttg atttataacc gtaagctaat
       61 agtaggcata cggacttttt gcgtcgatca gattcgtctc attagtttga aaactgtaaa
      121 gaatttgcga caggtactac cagctgaatg gcggtaagca ggaaaatatg gggaagagca
      181 agcaaatatc aattagcggg ggttaacaat caggcggcag agccgcctga tgagaaatta
      241 cttattcggt ttctcgattt cgccaagttc ttcgagaatt tcttccggtt ggtccgtcgg
      301 cggcgggact tcttccaccc acgcggcaaa cagacgccag gaaacggcta acagaaccgg
      361 accaataaac agaccgatca tcccgaaagc aatcaaacca ccaataacac cagagaggat
      421 caggatcagc ggtaaatcgg cacccatgcg aattaacatt gggcggatga cgttatccag
      481 cgtgccaacc acaccgctcc acactaacaa taccgttccc caggtggtat cgccagtcca
      541 gtagagccag ataatcgccg gaatcagtac cggcaacggg ccaagctgga caaggcagga
      601 gaggatcatt aacaccgtta gcaacgttgc ataaggtacg ccggatacgg cgaggccgat
      661 accgccaagc actgcctgta ctaacgccgt caccaccaca cccagcgcca ccgcgcggat
      721 agcctgtgcc gccagcagta cggcggcatc tccgcgaacg cctgccagac gggttgcaaa
      781 atggcgaatg ccttgtgcca cctgttcacc gcgccagtac agcagggcac tgaagagaag
      841 catcagcgca caatgcacca taaagcgccc gatatgcgcc gcctgcccaa cgaaccaggt
      901 ggtggtggtg ccaatataag ggcggacttt cgccatgatc gccgtccccc ccatatccag
      961 caagttgtgc cagcctgcat acagcttcgc gccaatcacc ggaatggtat taagccacgc
     1021 cagatcgggt aacgtcatgt cgccgctgga aatggcttta attagcgggc cgctgccgtc
     1081 gacgatactg ttaaccagca aagcaatagg gatgataaac accatcacta ataacagcgt
     1141 catcaccaga acggcgagag agcggcggcc aaacatgatc ttttgcaaac gtaacaatac
     1201 cggccaggtg gcgataacca ccgtaccggc ccatgcaaag ccgagaataa agggttgaac
     1261 aatccacaga catgccacaa tcatgatggc taaaaacagc accgaaagca gaatttgtgc
     1321 gacatccctg ggctgacgaa catttaccat aactactttt cacctttttt gtgcgtcaaa
     1381 tcgtcgacgc gacgtgaaca cgcgaaactc acctgcataa tgatgagcaa tttcagcggt
     1441 ttttaacagg ccgattctgc ccttaattct gatgggcgtg ttagaaaaaa atgtggtaca
     1501 acaactcagg caacacgcaa acgatttact cgtcgtattt caagttgcat gtgctgcggc
     1561 tacgttcgtt caccccagtc acttactatt gtaagctcct ggagttcact cgcttgccgc
     1621 cttcctgcaa ctcgaaatcc atagagtaaa taaacatcac ctttcagtac gctattgcag
     1681 ccgcctgaaa gaatagtgta tcgacctaca tcacagaccg caggaaaggg tcaatataat
     1741 gattccacag atttcccagg cacccggcgt cgttcaactg gtgcttaatt ttttgcaaga
     1801 gctggagcaa caaggtttta ccggcgatac ggcgacaagt tatgccgatc gtctgacaat
     1861 gtcgaccgac aacagtattt accaacttct ccccgatgcg gtggtatttc cgcgttcaac
     1921 cgcagatgtg gcgctgatcg cccgtcttgc cgcgcaggaa cgctattcat cgctgatctt
     1981 taccccccgc ggcggcggca ccggcaccaa cggtcaggcg ctcaaccagg ggattattgt
     2041 tgatatgtcc cgccatatga accgcatcat cgaaattaac cctgaagagg gctgggtgcg
     2101 cgttgaggcc ggggtgataa aagatcaact caatcagtac ctgaaaccgt tcggctactt
     2161 ttttgcgccg gaactttcga ccagcaaccg ggcaacgctc ggtgggatga tcaataccga
     2221 tgcttctggt cagggatcgc tggtctatgg caaaacgtcg gatcacgtac ttggcgtacg
     2281 tgcggtgttg ttggggggcg atattctcga tacgcaacct ttacccgtcg aactggcgga
     2341 aacgctgggt aaatccaata ccacaatcgg gcgaatttat aacacggttt atcaacgttg
     2401 ccgtcagcaa cgccagttaa tcatcgacaa cttccccaaa cttaaccgct ttcttaccgg
     2461 ttacgatctg cgtcatgtct ttaacgatga gatgaccgag ttcgacctga cgcgcattct
     2521 gacgggttca gaagggacgc tggcctttat taccgaagcg cggctggata ttacgccctt
     2581 gcctaaagtg cgccgtctgg tgaacgtcaa atatgactct tttgactccg cgctgcgtaa
     2641 cgcgccgttt atggttgagg cgcgggcgct ttcggtagag acggtggact caaaagtgct
     2701 gaatctggcg cgggaagata ttgtctggca ttccgtcagc gagttgatta ccgatgtgcc
     2761 tgacaaagag atgctcgggc tgaacattgt ggagtttgct ggtgatgatg aggcgctgat
     2821 tgacgagcga gttaatgctc tctgtgtgcg gcttgatgag ctgattgcca gccaacaggc
     2881 aggtgtgatt ggctggcagg tgtgccgcga gctggcgggt gttgaacgta tctatgcgat
     2941 gcgtaaaaaa gccgttggtc tgcttggcaa tgccaaaggt gccgctaagc caattccgtt
     3001 tgctgaggat acctgcgtac cgccggaaca tctggccgat tatattgctg aatttcgcgc
     3061 gctgctcgac agccacggct taagctacgg tatgttcggt cacgtcgacg caggtgtctt
     3121 gcacgtccgt ccagcgctgg atatgtgcga tcctcaacaa gagattttga tgaagcaaat
     3181 ctctgatgac gtagtggcgc tgactgcgaa atacggtggt ttgttgtggg gcgagcacgg
     3241 caaaggtttt cgcgctgaat acagcccggc gtttttcggt gaggaacttt ttgcagaact
     3301 gcgcaaagtg aaagcggcat ttgacccgca taaccgactc aacccaggga agatttgccc
     3361 gccagaaggt ctcgatgcgc cgatgatgaa agtggacgcg gtgaagcgcg gtacattcga
     3421 tcggcagatc cccattgcgg tacgccagca gtggcgcggt gcgatggagt gtaacggcaa
     3481 cggtttatgc ttcaactttg atgcccgtag tccgatgtgt ccgtcgatga agatcaccca
     3541 gaaccggatt cattcaccga aagggcgcgc aacgctggtg cgtgaatggc tgcgtttgtt
     3601 ggcggatcgc ggcgttgatc cactcaaact ggaacaagaa ctgcctgaat ccggcgtcag
     3661 tttgcggacg ttaattgccc gcacgcgcaa tagctggcat gcgaataaag gcgaatatga
     3721 cttctcacac gaagtcaaag aggcgatgtc gggctgtctg gcctgtaaag cgtgttcgac
     3781 ccagtgcccc atcaaaattg atgtgccgga gtttcgctct cgttttctgc agctctatca
     3841 cacccgttat ttacgcccgc tgcgcgacca cctcgtcgct acggtcgaga gctacgcgcc
     3901 gctgatggca cgcgcgccga aaacctttaa cttcttcatt aaccagccgc tggtgcgcaa
     3961 actctcggaa aaacatatcg gcatggttga tttgccgctg ctgtcggtcc cctcgctaca
     4021 acaacaaatg gtggggcatc gctcggcaaa catgacgctg gaacagcttg aagctctcaa
     4081 tgcagagcag aaagcgcgca cagtgttggt ggtgcaggac ccctttacca gctattacga
     4141 tgcgcaagtg gtggcggatt ttgtccgtct ggtcgaaaaa ttaggtttcc agcctgtgtt
     4201 actgccattt tcgccaaatg gcaaagccca gcatattaaa ggttttctta atcgttttgc
     4261 gaagacggcg aaaaagacgg cggatttcct caaccgtatg gcgaagctgg gtatgccaat
     4321 ggtgggcgtc gatccggcgc tggtactttg ttatcgcgat gaatataaac tggcgctggg
     4381 cgaggaacgt ggcgagttta acgtcttact ggcgaatgaa tggctggcaa gcgcacttga
     4441 gtcacagcca gtggctacag tcagcggtga atcatggtat ttctttggtc actgtaccga
     4501 agttaccgcc ttgccgggtg cgccagcaca atgggccgcg atatttgccc gttttggcgc
     4561 gaaactggaa aatgtcagcg tgggttgctg cggcatggca gggacttacg gacatgaagc
     4621 gaaaaaccat aaaaattcgc tcgggatcta tgagttatcc tggcatcagg ctatgcagcg
     4681 actgccgcgt aaccgctgtc tggcgaccgg atattcctgc cgtagccagg taaaacgggt
     4741 tgaaggcacg ggggtacgcc atcctgtgca ggctttactg gagattatta aatgatatgg
     4801 aaacggaaaa tcaccctgga agcactgaat gctatgggtg aaggaaacat ggtggggttc
     4861 ctggatattc gctttgaaca tattggtgat gacacccttg aagcgacaat gccagtagac
     4921 tcgcggacaa agcagccttt cgggttgctg catggaggag catccgtggt actggccgaa
     4981 agtatcggtt ccgttgccgg ttatttatgt accgaaggtg agcaaaaagt ggttggtctg
     5041 gaaatcaatg ctaaccacgt ccgctcggca cgagaagggc gggtgcgcgg ggtatgcaaa
     5101 ccgttgcatc tcggttcgcg tcaccaggtc tggcagattg aaatcttcga tgagaaaggg
     5161 cgtttgtgct gttcgtcacg attgacgacc gccattttgt gacgttgtca ccagaaaagt
     5221 gtgacgcata ttgcctttgg tcattatctg cacagtaaac ggtggtatac tgtgcaggtc
     5281 ttgtctaaaa gcgaggatgc tatgtctact caacttgatc ccacccagct ggccattgaa
     5341 tttttacgcc gtgaccagag caatctttca cctgcccaat atctcaaacg tctgaaacaa
     5401 ctagaacttg aatttgctga cctgcttact ctctcttctg ctgaattaaa agaagaaatc
     5461 tactttgcct ggcgtctggg cgtgcattga tcctgactta atgccgcaag ttcgccaaat
     5521 cgacctggat taacctccgt ccaggttcga tgaccttctt aatactgctt atctaccgcc
     5581 tgatattgtt caaaaataat actaatcgca gtttgtgtta aaacggcggg ttagctttat
     5641 gagattattc ttatcgcccc ttcaagagct aagccactga gagtgccgga gataagcgcc
     5701 ggatggggta gaaaccctta agcctgtgtt gcacagactt aagggttttc ttatttctga
     5761 acgctgcact tttttaccta tctcttaaat agtcattttc aatacaactt tttctttttc
     5821 atcacagcct taatgctcct tagataataa ccattatcta acaatgagat acctaattct
     5881 tagcgtgcct gttaacccac acatcagggt ctatgcttat taaaagcagg caaaaagcat
     5941 gttttacggt aaagcccctg cgtttgctgg gttgtactga taatcattat cactaacatg
     6001 ctgttatacg ctgaaagcga tgaagtgagg taaatcgatg gacatgcatt caggaacctt
     6061 taacccacaa gatttcgcct ggcaaggctt aacgctgaca cccgcagcgg cgatacacat
     6121 ccgtgagctg gtggcaaagc agccgggtat ggtcggcgtg cgcttaggcg tgaagcaaac
     6181 gggctgcgcg ggctttggct atgtgctcga cagtgttagc gagccggaca aagacgatct
     6241 gctgtttgaa cacgacggcg cgaagctgtt tgtcccgctg caagcgatgc cgtttattga
     6301 tggcacggaa gtcgatttcg ttcgtgaagg acttaatcag atattcaaat ttcacaaccc
     6361 taaagcccag aatgaatgtg gctgtggcga aagctttggg gtataggcgg tactatgtct
     6421 cgtaatactg aagcaactga cgatgtcaaa acctggaccg gcggcccgct gaattataaa
     6481 gaaggattct tcacccagtt agccaccgat gagctggcaa aggggataaa cgaagaggtg
     6541 gtgcgcgcaa tttcggcgaa gcgtaatgag ccggagtgga tgctgaagtt tcgtctaaac
     6601 gcctatcgcg catggctgga gatggaagaa ccgcactggt tgaaagcgca ctacgacaag
     6661 ctgaattatc aggattacag ctactactca gcaccatcgt gcggtaattg tgacgacact
     6721 tgcgcgtctg aacctggcgc ggtgcagcaa actggcgcga acgccttttt aagtaaagag
     6781 gtggaggcgg cgtttgagca gttgggcgtt cccgtgcggg aaggcaaaga ggtggcggtg
     6841 gatgccattt tcgactcagt ttcggttgcc actacttatc gcgaaaaact ggcggagcag
     6901 ggaattattt tctgttcctt tggtgaggcg atccacgatc acccggaact ggtgcgtaaa
     6961 tatctcggca ccgtggtgcc ggggaatgac aacttctttg ccgcgcttaa tgcggcggta
     7021 gcctctgacg gtacgtttat ttatgtgcct aaaggcgtgc gctgcccgat ggaactttcc
     7081 acctattttc gcattaacgc ggaaaaaacc gggcagtttg agcgcaccat tctggtggcc
     7141 gacgaagaca gctacgtcag ctacattgaa ggctgttccg ctccggtgcg tgacagctat
     7201 cagttacacg cggcagtggt ggaagtcatc atccataaaa acgccgaggt gaaatattcc
     7261 acggtacaaa actggttccc tggcgataac aacaccggcg gtattctcaa cttcgtcacc
     7321 aagcgtgctt tgtgcgaagg cgaaaacagc aaaatgtcat ggacgcaatc agaaaccggg
     7381 tccgcgatta cgtggaaata tcccagctgc attttgcgcg gcgataactc cattggtgag
     7441 ttttactcag tggcgctgac cagcggtcat cagcaagcgg ataccggcac caagatgatc
     7501 cacatcggta aaaacaccaa atcgaccatt atctcgaaag ggatctctgc cggacatagt
     7561 cagaacagtt atcgcggctt agtgaaaatc atgccgacgg caaccaatgc gcgcaatttc
     7621 actcagtgcg actcaatgct gattggcgct aattgtgggg cgcatacctt cccgtatgtc
     7681 gaatgtcgca ataacagcgc acaactggag cacgaggcaa cgacatcacg tattggtgaa
     7741 gatcaactgt tttactgcct gcaacgcggg atcagcgaag aagacgccat ctcgatgatt
     7801 gttaacggtt tctgcaaaga cgtgttctca gaactgccgc tggaatttgc cgttgaagca
     7861 caaaaactcc tcgccatcag tcttgaacac agcgtcggat aaggaataaa catgttaagt
     7921 attaaagatt tacacgtcag cgtggaagat aaagctatcc tgcgtggatt aagcctcgcc
     7981 gttcgtcccg gcgaagttca cgccattatg gggccaaacg gttcgggcaa aagtacctta
     8041 tcggcaacgc ttgccgggcg cgaagattat gaagtgacgg gcggcacggt tgagttcaaa
     8101 ggcaaagatt tgcttgcact gtcgccggaa gatcgtgcag gcgaaggcat ctatatggcc
     8161 ttccagtatc cggtggagat tccaggcgtc agtaaccagt ttttcctgca aacggcactt
     8221 aatgcagtgc gcagctatcg cggccaggaa acgctcgacc gctttgattt tcaggatttg
     8281 atggaagaga aaatcgctct cctgaagatg ccggaagatt tattaacccg ttcggtaaac
     8341 gttggttttt ccggcggcga gaaaaagcgc aacgatattt tgcaaatggc ggtgctggaa
     8401 ccggagttat gcattcttga tgagtcggac tccgggctgg atattgacgc attaaaagtg
     8461 gtcgccgatg gcgtgaactc gctgcgtgat ggcaagcgct cattcatcat tgttacgcac
     8521 taccaacgca ttctcgacta catcaagcct gattacgtcc atgtgctata tcagggacga
     8581 attgtgaaat ccggcgattt cacgttggtc aaacaactgg aggagcaggg ttatggctgg
     8641 cttaccgaac agcagtaacg cgctgcaaca gtggcatcac ttgtttgaag ctgaagggac
     8701 aaaacgctca ccgcaagcac agcagcattt acaacaattg ctgcgtaccg gactgccgac
     8761 acgtaaacat gaaaactgga aatatacgcc gctggaaggg ctgaccaata gccagtttgt
     8821 cagcattgcg ggagagatat ccccacagca gcgagatgcc ttagcgttaa cgttagacgc
     8881 tgtgcggctg gtatttgtcg atggacgtta cgtgtcagca ctgagcgatg cgactgaagg
     8941 cagcgggtat gaagtgagca ttaacgacga ccgtcagggg gtacccgacg ctattcaggc
     9001 ggaagtgttt ctgcatttga cggaaagcct ggcacaaagc gtgacgcata tcgccgtgaa
     9061 gcgcggtcaa cggccggcaa agccattgct gttaatgcat attacccagg gcgtggcagg
     9121 tgaagaggtc aacactgccc attaccgaca tcatctggat ctggcggaag gtgccgaagc
     9181 aacggtgatc gaacattttg tcagccttaa tgatgctcgt cacttcaccg gcgcacggtt
     9241 cactatcaat gtcgcagcga acgcccactt gcagcatatc aagctggcgt ttgaaaaccc
     9301 ggtcagtcac cactttgccc ataacgattt gttgctggct gacgatgcca ccgcatttag
     9361 ccatagtttc ctgctgggtg gcgcagtgtt acgacacaac accagtacgc aactcaatgg
     9421 cgaaaacagc acgctgcgga tcaatagcct ggcgatgccg gtgaaaaacg aggtgtgtga
     9481 tacccgtacc tggctggaac acaataaagg tttttgtaac agccgacagt tgcacaaaac
     9541 tatcgtcagc gacaaaggcc gcgcggtatt taacggtttg atcaacgtcg cgcagcacgc
     9601 catcaaaacg gatggtcaga tgaccaacaa taatctgctg atgggcaaac tggcagaagt
     9661 ggatacgaaa ccgcagctgg aaatctatgc agatgatgtg aaatgcagcc acggcgcgac
     9721 ggtggggcgt attgatgatg aacagatgtt ctatctgcgc tcgcgcggga tcaatcagca
     9781 ggatgcccag cagatgatca tttacgcctt tgctgccgaa ctgacggaag cactgcgtga
     9841 tgaggggctt aaacagcagg tgctggcccg aatcggtcaa cgactgccag gaggtgcaag
     9901 atgacttttt ccgtcgacaa agtgcgggcc gactttccgg tgctttcgcg tgaggtaaac
     9961 ggtttgccgc tggcttatct cgacagcgcc gccagtgcgc agaaaccaag ccaggtgatt
    10021 gactccgaag ccgagtttta tcgtcacggc tacgcggcgg tgcatcgcgg tattcatacc
    10081 ttaagcgccc aggcgaccga gaaaatggag aacgtgcgca agcgggcatc gctgtttatt
    10141 aatgcccgtt cggcggaaga gctggtgttc gtccgcggca cgacggaagg gatcaatctg
    10201 gtcgccaata gttggggcaa cagcaacgtg cgggcgggcg ataacatcat catcagtcag
    10261 atggagcacc acgctaacat tgttccctgg cagatgcttt gcgcacgcgt tggcgcagag
    10321 ctgcgtgtga tcccgctcaa tcccgacggt acgctgcaac tggagacgct acctaatctg
    10381 ttcgatgaga aaactcgcct gctggcaatt actcatgtct ccaacgtgct tggcacagaa
    10441 aatcccctgg cggaaatgat cacgcttgcg catcagcatg gcgcaaaagt gctggtggat
    10501 ggcgctccgg cggtgatgca tcatccggtg gacgttcagg cgctggattg cgatttttac
    10561 gtattctccg ggcataaact gtatggcccc accggaattg gcattcttta tgtgaaagaa
    10621 gccttgttgc aggagatgcc gccgtgggaa gggggcggtt ctatgatcgc caccgtcagc
    10681 ctgagtgaag gcactacctg gaccaaagca ccatggcggt ttgaagccgg tacacccaat
    10741 accgggggca tcattggtct tggcgcggcg ctggagtatg tttcggcgct ggggcttaat
    10801 aacatagccg agtatgaaca gaatctgatg cactacgcgc tatcacagct ggaatctgta
    10861 ccggatctca ctctctatgg cccgcggaac aggcttggcg ttattgcttt taatcttggt
    10921 aaacaccacg cctatgatgt tggcagtttt ctcgataact acggcattgc tgtgcgtacc
    10981 ggacatcact gcgctatgcc attaatggcc tattacaacg tccctgctat gtgtcgggcg
    11041 tcgctggcca tgtataacac ccatgaagaa gtggatcgtc tggtgaccgg actgcaacgt
    11101 attcaccgtc tgctgggata acagggaggc actatggctt tattgccgga taaagaaaaa
    11161 ttgctgcgta attttttacg ctgcgccaac tgggaagaga aatatctcta cattattgag
    11221 ctgggccagc gtctgccaga attacgcgcc gaagaccgaa gcccacaaaa tagcattcag
    11281 ggctgtcaga gtcaggtgtg gattgtcatg cgccagaatg cgcagggaat tattgaattg
    11341 cagggcgaca gcgatgcggc gattgtgaaa gggcttattg cggtcgtctt tattctctac
    11401 gatcagatga cgccgcagga tattgtcaat ttcgatgtgc gtccgtggtt tgaaaaaatg
    11461 gcgctcaccc aacatctcac cccatctcgt tcacaaggtc tggaagcgat gattcgcgca
    11521 attcgcgcca aagccgctgc acttagctaa actaaaggga cagctttcat ccggttggct
    11581 cacattgccg gatggtcaat gtttacactg acctgcacag gagtgttttg gtttcaggtg
    11641 aacataagcg ccatccgacg tttcagcgtg agtctccggc aaatacagga ggtttacaat
    11701 gaaacgcgcg tctttgctta cactcacgct tatcggcgct tttagcgcca tccaggctgc
    11761 ctgggcggtt gattatccgc taccaccaac cggaagccga ctggttgggc aaaatcaaac
    11821 gtatacggtg caagaagggg ataaaaacct tcaggctatt gcccgacgtt ttgatactgc
    11881 ggcaatgttg atccttgaag ccaataacac tatcgccccg gtgccaaaac ctggtacgac
    11941 gataactatt ccttcacaac tgttattacc tgatgcaccg cgtcagggga ttatcgttaa
    12001 ccttgcagag ctgcgccttt attattatcc gccgggagaa aatattgtgc aggtttatcc
    12061 aataggtatt ggattgcagg ggctggaaac gccggtgatg gaaacgcgtg ttgggcagaa
    12121 aatccctaac ccaacctgga cgcctacggc aggcattcgt caacgttcgc tggagcgtgg
    12181 cattaaatta ccgccagtcg ttcctgccgg accaaataac ccgctaggac gttacgcact
    12241 gcgcctcgcg catggtaatg gcgaatacct cattcatggt accagtgcgc cggacagcgt
    12301 cggtttgcgc gtcagttcag ggtgtattcg catgaatgca ccggatatta aagccttgtt
    12361 ctccagcgtg cggacgggaa cgccggtgaa agtgatcaac gaaccggtga aatattccgt
    12421 ggaacctaac gggatgcgtt atgttgaagt acatcgacca ctatcagcag aagaacagca
    12481 aaacgttcag acaatgccat acacactgcc agcaggcttt acgcaattta aagacaataa
    12541 ggctgtagat cagaagttag tcgataaagc gttgtatcgt cgggcagggt atccggtttc
    12601 ggtgagcagt ggagcaactc ccgcagccag caatgcgcct tcagtagagt cagcgcagaa
    12661 tggtgaacca gagcaaggga atatgttacg cgcgacgcag tagcggtaaa cgggagacaa
    12721 aaaaaatggc gcacaatgtg cgccattttt cacttcacag gtactattac ttgcggtatt
    12781 tagtagccat gttgtccaga cgctggttag cacgagctgc gtcatcttta gcagcctgaa
    12841 cgtcggaacg cattgcgttc acgtcgttgc tcagctggtc aactttagcg ttcagagtct
    12901 gaacgtcaga agacagctga tcgattttag cgttgctgga gcaacctgcc agcagagtag
    12961 aacccaggat taccgcgccc agtaccagtt tagtagcttt cattattaat accctctaga
    13021 ttgagttaat ctccatgtag cgttacaagt attacacaaa gttttttatg ttgagaatat
    13081 ttttttgatg ggaaggcact tatttttgat cgttcgctca aagaagcatc gaacgctggg
    13141 ttttagataa aaaaattgag agaaaacagg atgcttccat cggattcatc ttagataaag
    13201 tgagattata tagtgaaatc cgatttgatt ttttattgct tctggttatc gattaaataa
    13261 aaaaagcgcc catcagggcg cttcgatata caaattaatt cacaaaagca atattacagg
    13321 acgtgaacag atgcggtgtt agtagtgccg ctcggtacca gtgcaccaga aaccataact
    13381 acaacgtcac ctttgtgtgc cagaccgctc tgcagagcca gttctttacc cagacggtag
    13441 aaatcatcag tagaagtgat ctctttaacc agctgcggca caacgccttt gctcagtacc
    13501 aactgatgag ccgttttttc gttggtggtc agtgccagga tggtggcatc cgggaagtat
    13561 ttacgtactg cgcgagcaga tttaccgccc tgggtagcaa ccacgatcag cggagcatcc
    13621 agtttttcag cagtttcaac ggcaccacgg cataccgctt cggtaatgcg cagtttacgg
    13681 ttgtcattgt tgaactcgag acggctgttc atcacgcggt cggtacgttc gcagatggtc
    13741 gccatgatag aaaccgcttc cagcgggtat ttaccttttg cggattcacc agacagcatc
    13801 actgcgtcag taccgtcgag gatggcgttt gcaacgtcac cggcttctgc gcgagtcggg
    13861 cgtgggtttt tgatcatgga atccagcatc tgggtcgcag tgataacgac tttacgtgca
    13921 cggatacatt tttcgatcat catcttctgg gcgaagataa cttcttctac cgggatttct
    13981 acacccaggt cgccacgcgc aaccatgatg ccgtcagagg cttcgaggat ttcgtcgaag
    14041 ttgttgaggc cttcctggtt ttcgattttg gagatgatgt ggatgttttc gccgccgtgc
    14101 gctttcaggt gctcacggat ttcgataacg tcagaacgct tacgaataaa ggaagcagca
    14161 acaaagtcta cgccttgttc gcaaccaaag atcaggtcct gtttgtcttt ttcagccagt
    14221 gctggcagag caatggaaac gccaggcagg ttcacacctt tgttttcgcc caggtcgccg
    14281 ttgttcagta ctttacagat aactttgtta ccttcaatgg cggtaacttc cataccgatc
    14341 agaccatcgt caaccagtac ggtgttgcca acagacaggt cagtagtgaa accttcatac
    14401 gttaccgcaa ccatttcgct gttgccgata acagatttat cagtggtgaa agtaaaggtc
    14461 tgaccagctt tcagagaaac gtcgttaccg ccttccagtt tcatggtgcg gatttccgga
    14521 cctttggtat caagcaggat agcggcggtt ttaccagttt tgctcatcac gttgcgcaga
    14581 ttctgaatgc gctgaccgtg ttctgcatag tcaccatgag agaagttcag acgcataacg
    14641 ttcatgccag cgtccagcat tttagctaac atctcttcag attcggtttt cggtccgatg
    14701 gtgcaaacaa ttttggtctt tttcatgaca gtcttagtct ttaagttgag aaggatggga
    14761 gaaacttgct ttctgggcgc actgccggaa agcgaatccg gtattacgaa agtggtggtg
    14821 cctcgctcta aggataggtg acatcgaaaa agcgtgcaga ggaatgtgtg cttgtgtctc
    14881 agcccaacgt gagtgcctgt ttttgagtcg ttgagttcta cagtccaaag tgctgaaacc
    14941 attcaagagt caattggcgc gcattatacg ctgaaattaa tccaaaagga aaacaaaaac
    15001 agcgtttcaa aaaatataca acttgttaga caataaattg ttatcaaaga tttaacgttc
    15061 cagggaaaag gttacgcaaa aacttcactg tttgcgcaac gtctggtgcc gaagttgtca
    15121 gtgtgaggat aagtgaaagt tcatccggtg tataaatata tcatcgctca attgctacag
    15181 ttaaaataat ttatcgcgtt atttgtctga ttttgtttga tttttgttta attggttaga
    15241 ttgatatatg ctgatagttg gcatgataaa taggtataaa gaatgggaaa tcggacaaaa
    15301 gaggatgaac tgtaccgaga aatgtgcaga gttgtcggta aagttgtgct ggaaatgcgc
    15361 gatctggggc aggaaccaaa gcatattgtt atcgcaggcg tattacgtac agcattagcc
    15421 aataaacgca ttcagcgcag tgagctggaa aagcaggcga tggaaaccgt cattaacgca
    15481 ctggtaaaat gaccattcag gatgaggcgg taagcttcat cctgatattc ttgcttcagt
    15541 tgcggtccag ttaatcattc taaataacac ctgctgaaac tatacgaatt tcggtatgta
    15601 ttttaattta tagcgataat gttattttta ttttctgcaa tgaacgtcac aaataattgt
    15661 cgctta
//
LOCUS       DAJOWB010000110        15170 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJOWB010000110 DAJOWB010000000
VERSION     DAJOWB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.110"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            41..313
                     /locus_tag="OHO85_003702"
     CDS             41..313
                     /locus_tag="OHO85_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCQ2673021.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKTRQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(314..1186)
                     /gene="rluF"
                     /locus_tag="OHO85_003703"
     CDS             complement(314..1186)
                     /gene="rluF"
                     /locus_tag="OHO85_003703"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCQ2673022.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEVKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1534..2481
                     /locus_tag="OHO85_003704"
     CDS             1534..2481
                     /locus_tag="OHO85_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCQ2673023.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELRIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2551..3357
                     /locus_tag="OHO85_003705"
     CDS             2551..3357
                     /locus_tag="OHO85_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCQ2673024.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSKRASYITGVTTNIAGGKTRG"
     gene            3367..3774
                     /locus_tag="OHO85_003706"
     CDS             3367..3774
                     /locus_tag="OHO85_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCQ2673025.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            3774..4268
                     /locus_tag="OHO85_003707"
     CDS             3774..4268
                     /locus_tag="OHO85_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCQ2673026.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4334..5131
                     /locus_tag="OHO85_003708"
     CDS             4334..5131
                     /locus_tag="OHO85_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCQ2673027.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLNLSLLEFGGVGVIMALVYIQLNPQWRKAEPQPQATTSTALDQLDD"
     gene            5142..5966
                     /locus_tag="OHO85_003709"
     CDS             5142..5966
                     /locus_tag="OHO85_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCQ2673028.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            6018..7247
                     /gene="sorE"
                     /locus_tag="OHO85_003710"
     CDS             6018..7247
                     /gene="sorE"
                     /locus_tag="OHO85_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCQ2673029.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            7630..8319
                     /gene="pepE"
                     /locus_tag="OHO85_003711"
     CDS             7630..8319
                     /gene="pepE"
                     /locus_tag="OHO85_003711"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCQ2673030.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8410..10041)
                     /locus_tag="OHO85_003712"
     CDS             complement(8410..10041)
                     /locus_tag="OHO85_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCQ2673031.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10261..13944)
                     /gene="metH"
                     /locus_tag="OHO85_003713"
     CDS             complement(10261..13944)
                     /gene="metH"
                     /locus_tag="OHO85_003713"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCQ2673032.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDAANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14144..14968
                     /gene="iclR"
                     /locus_tag="OHO85_003714"
     CDS             14144..14968
                     /gene="iclR"
                     /locus_tag="OHO85_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCQ2673033.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3606 a         4005 c         3970 g         3589 t
ORIGIN      
        1 tcccgtacac tgaactctct ggctcactga gggcaccatc atggcactcc cccgtattac
       61 ccaaaaagag atgactgaac gcgagcagcg tgaacttaaa acactgctgg atcgggcgcg
      121 gattgcgcat ggtcgcgtac tgaccaactc cgaaaccaac agcattaaga aagagtacat
      181 cgataaatta atggtcgagc gtgaggctga agcgaaaaaa acccgccagt tgaagaaaaa
      241 gcaggcttat aaaccggatc ctgaagcgtc atttagctgg tcagcaaata cgtcgacgcg
      301 cggcaggcgc tgattagcgt cccttctttt tacgccccgg cgaggtaaaa cgcttaccgt
      361 tggacgccgg gcgaccgcct ttttccgccg ttttttccat cttaacgact ggacgtttga
      421 tgcccgctgt tttcggttta gccttcgctt taggttttac ctcggaagag gaattttcaa
      481 tgagtttaaa caggtcgatt aactcatcgt cagttaaatc gcgccactca cccagcggaa
      541 tgccgcttaa actgacgttc ataatgcgcg tacgttccag ctttttcact tcatagccga
      601 aatgctcgca catgcgccgg atctgacggt tcagcccctg caccagggta atgcggaaga
      661 caaacggcgc ttcttttttg actttgcact ttttcgtcac cgtcccgagg atcggcaccc
      721 ccgcccccat accacgaata aattcatcgg taatcggttt atcgaccgtc accagatact
      781 ctttctcatg atcgttaccc gcacggagga tcttattcac cagatcgccg tgattggtga
      841 ggaaaatcag cccctgggag tctttatcca ggcggccaat cgggaacacg cgtttgctgt
      901 ggttaacgaa atcgacaatg ttatcgcgct cgccatcttc ggtggtgctg acaataccga
      961 cgggcttgtt cagggcgata agtaccaaat cttcggcttc ccgaggctca atcaactgac
     1021 cgtttacttt cacaatgtcg ccgggtttca cctgatcgcc aatggtggct cgcttgccat
     1081 taaggaacac attgccttgc tcgatatagc gatccgcttc gcggcgtgag caaattccgc
     1141 tttcgctgat gtatttattt aaacggactg atgagtcggg cagcataaat tctcctgtaa
     1201 aacgcggaat ataccgtacc tcttagcggg gaaaaacagg gttatgcggc aacgtcgaaa
     1261 ttaaatgtga tctgccacgc cttttatcac aaaagtgtct ccggaagtac gatgcaaatt
     1321 tgcctggcaa aataaggata atcaacatat tacgaatcgc tgcacacctg tgctgatgac
     1381 tactacgcag agaaacaaat gtgcaagaga aagcgctatt tctccccatg caaagagtga
     1441 aaaatcgctt tgaaaggccc tcatgatggg gttatatcag atcaagcggt gagaaagcgt
     1501 atttgcacaa atgtgcagaa ccaggaggcg gcaatggaaa acagtgacga tatccgtttg
     1561 attgtgaaga ttgcccaact ctattacgaa caggatatga cgcaggcgca aatcgcgcgc
     1621 gaactgcgta tttaccgcac caccatcagc cgcttgctta aacgaggccg cgatcaggga
     1681 attgtcacca tcgccatcaa ctatgactac aacgaaaatc tctggctgga gcagcaactg
     1741 aagcaaaagt ttggcctgaa agacgttgtg gtggtgtcgg gaaatgatga ggatgaagag
     1801 actcaactgg cgatgatggg gttacacggc gcgcaactgc tggatcgctt gctggagcct
     1861 ggcgatattg tcggtttttc ctggggtcgc gcggtgagcg cactggttga aaacttgccg
     1921 caggcggggc aatcgcggca gttaatctgc gtaccaatta ttggcggacc gtccggtaaa
     1981 ctcgaaagcc gctatcatgt aaacacatta acctacagcg cggcagcgaa gctgaaaggg
     2041 gaatcgcatc tcgcggattt tccggctctg ctggataacc cattaattcg taatgggatc
     2101 atgcagtctc agcactttaa aaccatctct gcctactggg ataatctgga tgtcgccctg
     2161 gtgggaattg gctcaccggc cattcgcgac ggcgctaact ggcatgcgtt ttatggtggt
     2221 gaagagagtg acgacctgaa tgcccgccag gttgctggcg atatttgctc gcgctttttt
     2281 gatattcacg gcgcaatggt tgaaacgaat atgagcgaaa aaacgctctc tatcgaaatg
     2341 aataaattaa agcaggcgcg atattccatt ggcattgcca tgagtgaaga aaaatacagc
     2401 ggaattattg gtgcactgcg tggaaaatat attaattgtc tggtaacgaa tagcagcaca
     2461 gctgaactat tactgaaata aaaatcttcc cgatgtttac cgtaataaaa attacggcgg
     2521 gtccctatta tctaaaatca ggagtctgtt atgcaaacgt ggttaaattt gcaggataaa
     2581 atcattatcg tcaccggcgg cgcatccggt attggtctgg cgattgtaga ggaattatta
     2641 gcacaaggcg caaacgtaca gatggtagat attcacggtg gcgatggtca atatgaaggt
     2701 cataaaggct atcagttttg gccgaccgat atttccagcg ccaaagaggt aaatcatacg
     2761 gtagcggaaa ttatccagcg ttttggtcgc atcgacggtc tggtcaataa cgccggggtc
     2821 aatttcccgc gtctgctggt cgatgagaaa gcgcctgccg ggcagtatga actcaacgaa
     2881 gctgcattcg aaaaaatggt caatatcaac cagaaaggcg tttttctgat gtcgcaggcg
     2941 gtggcgcgac agatggtcaa acaacatgat ggcgtgattg tgaatgtttc ctcagaaagt
     3001 ggactggaag gctcagaagg ccaaagctgt tacgccgcta ccaaagccgc gcttaatagc
     3061 ttcacgcgct cctggtcgaa agagctgggt aaacacggta tccgtgtggt cggtatcgcg
     3121 cccgggattc tggaaaaaac cgggctacgc acgccggaat atgaagaggc gctggcgtgg
     3181 acgcgcaata tcaccgtcga gcagctgcgt gaaggctata ccaaaaacgc cattcctatt
     3241 gggcgcgccg gaagattagc agaagtcgct gattttgttt gttatctgtt gtctaaacgc
     3301 gccagctaca tcaccggagt aaccactaac attgcgggcg gcaaaacgcg cggctaagga
     3361 ggctttatgg tcaacgccat tttttgtgcc cacggcaaat tagcttgcgc catgctggaa
     3421 tcggttcaga tggtttacgg caacgccaat gttgaagccg ttgaatttgt gcccggcgag
     3481 aacgccggag acatcgtagc aaagctggaa aagttagtaa gcattcacaa tcaagatgag
     3541 tggctgattg ccgtggattt acagtgcggc agcccgtgga atgcggcagc gatgcttgcg
     3601 atgcgcaatc ctcgtctgcg ggtaatcagc ggcctttctc tgccgctggc gctcgaactg
     3661 gttgataacc aggacagcat gaatgttgat gaactgtgtg aacacctgac gcaaatcgca
     3721 aagcaaacct gcgttgtctg gaaacaactg gcaacggcag aggaggattt ctgatgaaca
     3781 ttacgctcgc ccgcattgat gaccgcctga tccacggtca ggtcactacc gtctggtcga
     3841 aagtggctaa cgcccagcgc attattatct gcaatgacga agtttataac gatgaagttc
     3901 gccggacatt attgcgtcag gctgcgccac cgggaatgaa ggttaacgtg gtcaatattg
     3961 aaaaagccgt cgctgtttat cacaacccgc aatatcagga cgaaaccgtt ttttatcttt
     4021 tcactcgccc acaggatgcc ttagcgatgg ttcgccaggg ggtaaaaata gacaccctga
     4081 atattggcgg catggcctgg cggcccggta aaaaacagtt aaccaaagcg gtttcattag
     4141 atgatgatga tattaacgca tttcatgagt taaataatct tggcgtcata ctggatttac
     4201 gcgtcgtagc gtcagatccc tcaatcaata ttatcgacaa aataaacgaa cagttaatcg
     4261 caaattaaat aatagcgcct gtgtacatat gctttaacag gcagggatga cgacacttaa
     4321 aaggtgaaaa atcatggaaa taagtaccct acaaatcatt gctatatttc ttttttcctg
     4381 tattgccgga atgggcagcg tgctggatga atttcagact catcgcccgt taattgcctg
     4441 tacggtgatt ggtttaattc tcggtgattt aaaaaccgga ataatactcg gtggtacgct
     4501 ggaattgata gctctcggct ggatgaacgt cggcgcggcg caatctccgg attctgcact
     4561 cgccagcata atctccgcca ttctggttat cgttggtcag caaagcatcg ccaccggaat
     4621 cgccatcgcg ttgcctgtgg ctgcggcagg ccaggtgctg actgtgtttg cccgtaccat
     4681 caccgtagtg ttccagcacg cggcggataa agcagcggaa gaagcacggt ttcgcactct
     4741 cgatattctg catgtctccg cgcttggcgt gcaggcgctg cgcgttgcta ttccggcact
     4801 gattgtctca ctgttcgtca gcgccgatat ggtgagcaat atgctgagcg cgataccgga
     4861 attcgtgacg cgcggtctgc aaattgctgg cggttttatc gtggtggtcg gttacgccat
     4921 ggtgctacgt atgatgggcg tgaaatattt gatgcctttc tttttcctcg gtttcctcgc
     4981 aggtggctac ctcaatctca gtctgctgga gttcggtggc gtcggcgtga tcatggccct
     5041 ggtctacatc cagctaaatc cacagtggcg taaagctgaa cctcaacccc aggccaccac
     5101 ctccaccgcc cttgaccaac ttgacgatta acggagccat catggaacag agaaaaatta
     5161 cacgcagcga tctggtgagc atgtttctgc gctccaacct gcaacaggcg tcctttaact
     5221 ttgaacgtat tcacgggctg ggcttttgct acgacatgat ccccgccatc aagcgacttt
     5281 acccattaaa agaggatcag gttgcggcgc tcaggcgaca cctggtgttc ttcaatacca
     5341 cgccagccgt atgtggcccg gtcatcggcg tcactgccgc catggaagag gcgcgggcca
     5401 acggcgcgga aattgatgac ggtaccatta acggcatcaa agtcggtctg atgggaccat
     5461 tggcaggggt tggcgatcca ctggtttggg gaacgctgcg cccgattacc gctgcgctcg
     5521 gcgcatctct ggcactttct ggcaacatcc tcggcccgct tttgtttttc tttattttca
     5581 atgcggtgcg tctggcgatg aagtggtatg gcctacagct cggctttcgc aaaggggtga
     5641 atatcgtcag cgatatgggc gggaatgtgc tgcaaaaact caccgaaggc gcgtcgattc
     5701 tcggactgtt tgtgatgggc gtgctggtca ccaaatggac gtcaatcaac gtaccgttgg
     5761 tggtttcaca aacgcatgcc gccgatggct ccaccgtcac catgaccgtg cagaacattc
     5821 tcgaccaact ttgccctggt ttgctggcgc tcggtctgac gctgcttatg gttcgtctgc
     5881 tcaacaaaaa aattaacccg gtatggctga ttttcgccct gtttggctta gggattatcg
     5941 gtaatgcgct gggcttcctg tcctgattct tcgccccggc acgactgccg gggccatcgc
     6001 tcaacatgag gtggtttatg aaaacaacag ctctgcgtct ttatggtaaa cgtgatttac
     6061 gcctggaaac ctttgacctt cctgaaatgc aggaggatga aatcctcgcg acggtggtca
     6121 ctgacagcct gtgcctctct tcctggaaag aggccaatct gggtgaaaac cataaaaaag
     6181 tacccgacga tgtggcgacc aaccccatca tcatcggcca cgagttttgc ggcgatattc
     6241 tggccgtggg taaaaagtgg cagcacaaat tccagccggg tcagcgttat gtgattcagg
     6301 ccaacctgca actccccgac cgcccggact gccccggcta ctccttcccg tgggtgggcg
     6361 gcgaagccac gcatgtggtt attcccaacg aggtcatgga acaagattgc ctgctggcat
     6421 acgacggcga aacctatttt gaaggctcac tggttgaacc gctttcctgc gtgattggcg
     6481 cgttcaacgc caactatcat cttcaggaag gtagttataa ccacacgatg gggattcgcc
     6541 cgcaagggcg catgctgatc ctcggcggca ccggaccaat gggactgttg gcgattgatt
     6601 atgcgctaca tggacccgtt aacccgtcgc tgctcgtcat taccgatacc gacaacgata
     6661 aattgagtta tgcgcgcaag cactatccat cagaaccgca aacactgatt cattatctca
     6721 atgccgccga tgcagcattt gatacgctaa tggcgctgag tggcggtcac ggcttcgatg
     6781 atattttcgt ctttgtgcct aatgaaggac tggtgactct cgcctcttcc ttgctggcga
     6841 cagatggttg cctgaatttc ttcgccggac cgcaggataa acatttcagc gcgccaatta
     6901 atttctacga tgtgcattat gcatttaccc actacgtggg cacgtcaggc ggcaataccg
     6961 acgacatgcg cgcggcggtc aaactgattg aagagaaaaa agtgcaggcc gcaaaagtgg
     7021 taacacatat tcttgggctg aatgccgcgg gcgaaaccac gcttgaattg cctgccgtcg
     7081 gcggcggcaa aaagctggtg tataccggga aatacctgcc gctgacgtca ctcacgcaga
     7141 ttcaggatca agcactggcg gcgattctgg cgcgtcatca ggggatctgg tccggtgagg
     7201 cggagcaata tctgctcact catgcagagg caatttccca tgattaatcg cgctacttaa
     7261 ctctgcatgt cgctggctgg tcgccgcggc aacttcgcga cctgatgtgc ggctgattgc
     7321 tgaagcggcc caatgtgcac gagaaggata aaaaaacatc agcgccttaa ggggcgctca
     7381 aattggtggc aaagtacaca ttgtttatgc cggatgcgga ataaagcctt attcggccta
     7441 cataatctcg aaaattcaat atattgcagt tatctggtag gcctggtaag cgtagcgcat
     7501 caggcaatgc tgtgtttgtc atcagtctca gcgcccggtg caatacgtga catcgtgagc
     7561 catgtcagta agtggttaaa ttcgcagtgg tagcatgcct cccgtgattt cttactaact
     7621 ggagaagtaa tggaactgct tttattgagt aactcgacgc tgccgggtaa agcctggctg
     7681 gaacatgcgc tgccgctgat tgctgaacag ttgcagggtc gccgttcagc ggtatttatt
     7741 cctttcgctg gcgtaacgca gacctgggat gattacacag cgaaaacggc tgcggttctc
     7801 gctccgctgg gtgtatctgt aaccggtatt catagcgttg tcgatcccgt tgccgcgatt
     7861 gaaaatgctg agatcgtgat tgtcgggggc gggaatactt tccagttgct gaaacagtgc
     7921 cgcgagcgcg ggctgctggc accaattact gacgtggtta aacgtggtgc gctgtatatt
     7981 ggctggagcg caggcgctaa ccttgcttgc ccaaccattc gtaccaccaa cgatatgccg
     8041 attgtcgatc cgcaaggttt cgatgcgcta aatctgttcc cgctgcaaat caacccgcac
     8101 ttcaccaacg cgctgccgga aggccataaa ggtgaaaccc gtgagcagcg tattcgcgaa
     8161 ctgctggtcg tcgcgccaga actgacgatt attggtctac cggaaggtaa ctggatcaca
     8221 gtgagtaaag gtcacgctac gctgggtggc ccgaacacca cttatgtgtt taaggctggt
     8281 gaagaagcgg tgccgctaga agctggtcac cgtttttaag taaattgccg gatgcatgat
     8341 ccggcatctt gttcatagtg cctgatgcga cgcttgacgc gtcttatcag gcctacagga
     8401 ttcacaatat taatactcgt cccgtccttc atcttcatcc ggctgttcca gcacactgta
     8461 agccaccgaa caaaacagcg agttcaggcg ctgcatatct cccagtaagc ctaaatgtag
     8521 cgaactggtt tcaatgctct gcacgttttg ctgatgcagg cgatcgacgt gggcgtggga
     8581 atagcggcga ttaagaatgc gaaaacgatg tttgctgcga cgcaaacgac gagcgctggt
     8641 gacatcgcca gagaagaaca ccgacattgc cagctttaaa ttactgagca attgctcata
     8701 gagcgcatcc agttccttca acccatcaag cgaaaatgcc cgccgtgctg ccagcgattt
     8761 atcggcaatt tcgctgccca tgcgctcgac gatatcggaa gcctgttcaa ggttgagcga
     8821 catttcaata atctccgccc agcgacgcga ctcttcttct gccagttcct cttttggcat
     8881 ccgcgccaga tacagcttaa tggcggtata gagaacgttg atatcatctg ccagcttacg
     8941 cagctctttc tcctgccgtg gctcgccgtg catcacttta ttcagccctt ccatcatctg
     9001 ttccatggcg tcgccaatgc gcagggtttc gcgcgcggcg ttcgccagag caagcgtggg
     9061 cgtatccagc gcgctgacat ccagatgttt aggccgtagc tgggtatcca gttccggttc
     9121 atcgcgaata atcgttttgc aaaaccgtgc catcgggtca acaaatggca gcatgaccag
     9181 gcaacgtaca aggttgtaga agacgtggaa atagatcacc agttccgctt ttggcaatga
     9241 cagcttcccc attgtctctg ccagcaaatg gacaaacggc aggataatca ggctacccac
     9301 cagcttaaac agcagactac ccagcgcgac acggcgggct gcggcattgg cggcactgtt
     9361 gttgagcatc gccagcaggc cggaaccgag gttagcacca atcaccagac agagcgccac
     9421 ggggaaggag ataatgcctg cggcggtcag agtcgcagtc agcagtacag cagcaaggct
     9481 ggagtagctg ataatggcga acatcgcgcc aatcagcgca tccagcagaa tatcgccggt
     9541 cagcgaggca aagatcacct gaacgccgtt tgcctgggtg atcggcgtta cggcctgcac
     9601 aatcaactcc agcgccagta aaatcagccc aagaccaata ccgacgcggc ccagttgccc
     9661 ggcgcgtgac tgtttgcgtc cgaggaaaaa aatcacgccg ataaaaataa gtaacggtga
     9721 cagccaggat aagtcgaagg tgagaatacg cgccattagc gccgtcccga catctgcccc
     9781 cagcacaatg accagagccg gtgcgagggc taccagatcc tgagcgacaa acgaggtcac
     9841 cagcatggtg gtggcattac tgctctgcac cagtgcggta acgccgatcc ccgcgcaaaa
     9901 ggcgagcggc tttttttcga cgctccggct aaggacagta cgcaaacgcg cgccgaagac
     9961 gcgcattacg ccggttcgaa caatatgagt cccccagacc agcagggcga cggcagaaag
    10021 caggtgaagc agcgttaaca cgagttcagg ttctccttat cgttatatgt tcctgaagca
    10081 attgaatgac caaaggaccg gaaacacgct gactggcatc tttactgtgc cttttgacat
    10141 tcgtttttcc ggctaagatt attccagcct gtttccagta taagtgtttc atattaataa
    10201 agagacaggg cactcattga gcgccctgtt tgttgtaagg aaaagtgaca gatttgtgaa
    10261 tcagtccgcg tcatacccca gattcggtgc cagccagcgc tcaacatcgg agacgctcat
    10321 acctttacgg cgggcatagt cttcaacctg atcgcgctga atttgcgcca ccgcgtagta
    10381 cttgctgtcc gggtggctga aataccaacc cgaaaccgat gcaccgggcc acatggcgaa
    10441 agattctgtg agtttcatgc cggtatgttt ttccacttcc agcagctccc agatggtggc
    10501 tttttccgta tgttccgggc aggccgggta gcccggtgcc ggacggatgc cctggtagtt
    10561 ttcgcggatc agttcttcgt tgctgaggtt ctcgttcggc gcatagcccc agtagacttt
    10621 acgcacacgc tcatggagat actccgcaaa ggcttcggct aaacggtcgg caagcgcttt
    10681 caccatgatt ttgttgtaat catcgtgctg cgcttcaaag gcatcagcca gtgcgtcctc
    10741 ttccagcccg ccagtcacgg caaatgcgcc gatgtaatct gctttaccag aaagcttcgg
    10801 cgcaacgaag tcagcgagac agtagttagc gaatcccgtt ttttcggtct gttgacgcag
    10861 atggtggctg acgttgatca catgggtgcg cgtttcgtca cggtagattt caatgtcatc
    10921 gcccacacgg tttgccggga acaggcccac cacgccacgc ggattcagcg ttttctcggc
    10981 gcttaattta tccagcatgt cgttggcgtc tttaaacagc cgctgcgcct caacgcccac
    11041 cacttcatct tccagaatgc gcggatactt cccggccagc gaccaggtca taaagaacgg
    11101 tgtccagtcg atgtaattac gcagagtttc gatgctggct tcaacttcct gcacgccgag
    11161 acggtgcgcc accggcggcg tgtaagcctg ccagtcaaaa gcgaaatcgt tatcgcgcgc
    11221 cgcttccagc gtgaccggtg gtgtgcgcgg tttcttacgc ccgtgctgaa tacgtacggt
    11281 ttcgtactcc ttgcgggtac gagcgacaaa atcatcacgc tgagtatcgg aaagcagcgc
    11341 cgccaccaca ccaacggtgc gcgatgcgtt ctgtacatac accgtcgggc cgctgtagtt
    11401 ctgctcaatt ttcaccgccg tgtgcgcttt tgaggtcgtc gcgccgccaa tcaatagcgg
    11461 gatggtgaag ccctgacgct ccatctcttt cgccacgtta accatctcgt ccagcgacgg
    11521 cgtgataagc cccgaaagtc caatcagatc agcattcact tctttagcgg tacggagaat
    11581 tttttccgca ggcaccataa cgccgagatc gacaatttcg tagttgttac attgcagcac
    11641 cacaccaacg atatttttac cgatgtcgtg gacgtcgccc ttcacggtgg cgatcaccat
    11701 cttgccgttg gttttgccct gctctttgct ggcttcaata aacggttcga gatacgccac
    11761 cgcctgtttc atgacgcgcg ccgatttgac cacctgtggc aggaacattt tcccttcgcc
    11821 aaacagatcg ccgaccacgt tcatgccgtc catcaacggg ccttcaatca cttcaatcgg
    11881 gcgcgtagcc tgctggcggg cttcttcggt atcctgctcg ataaactcgg taatgccttt
    11941 gaccagcgag tattccagac gtttattcac ttcccacgag cgccactccg cctgctgggc
    12001 gttagcggcg tcgtcggttt tgctgccgcg atatttctcg gcaagctcca gtaaacgctc
    12061 agtgccatca tcgcgacgat taagaatcac atcttccacc gcgtcgcgca gttcagcggg
    12121 caggtcgtcg taaatcgcca gctgcccggc attgacgatc cccatatcca tgccattgcg
    12181 aatagcgtag tagaggaaca ctgcgtgaat ggcttcgcgc accggatcgt tgccacggaa
    12241 cgagaaagaa acgttagata cgccgccgga aattagcgcg tgcggcagtt cgcgtttgat
    12301 gtcttcacac gcgccgataa agtcctgcgc gtagttgttg tgctcttcaa tgccggtggc
    12361 aaccgcgaag atgttcgggt cgaagatgat atcttctggc gggaagccaa cctcttcggt
    12421 gaggattttg tacgcccgac ggcaaatctc gattttccgt gcgcgggtat cggcctgtcc
    12481 ctgttcgtca aaggccatta ccaccactgc cgcaccgtaa cgacgcaaga gcttcgcgtg
    12541 atggataaag gcgtcgacgc cctctttcat cgagatagag ttaacaatgc ctttgccctg
    12601 gatacacttc agaccttttt caatgacatc ccattttgag gagtcgatca taatcggcac
    12661 gcgagcgata tccggttcac cggcaatcag attgagaaaa cgcaccatcg ccgcttcggc
    12721 atcgagcatc ccttcatcca tgttgatatc gataatctgc gcaccgtttt ccacctgctg
    12781 gcgggccaca tccagcgcct cgctgtattt ctcttctttg atcaggcgct tgaacttagc
    12841 ggaaccggtg acgttagtcc gctcgccgac gttcacaaac aggctgtcgt cgccaatgtt
    12901 cagcggctcc aggccggaca aacggcaggc tacgggaatt tcaggcagtt tgcgcggcgc
    12961 taatccttct actgcacgac tcatcgctgc aatatgttgt ggcgtggtgc cacagcagcc
    13021 gccgacgata ttgagaaaac ccgcttgcgc ccattcacgt atctgttttg ccatcgtgtc
    13081 ggcgtcgaga tcgtactcac caaaggcgtt gggtagcccg gcgttcgggt gcgcggtgac
    13141 gtagcattcc gcaatccgtg acagctcctg cacgtactgg cgcagttcat ctggccccag
    13201 cgcacagttc aggccaaagg tcagagcttc ggcgtggcgc aatgagttgt aaaatgcttc
    13261 ggtggtctgc ccggagagcg tgcgcccgga ggcgtcggtg atggtgccgg agatcataat
    13321 cggcagctca acgcccagcg cttcaaactc cgttttcacc gcaaagaccg ccgctttggc
    13381 gttaagggtg tcgaaaacgg tttcaatcag gatcagatcc gcaccacctt ccaccagcgc
    13441 tttggtggac tctcgataag ccgccaccag cccgtcaaaa gtgatattac gaaatgccgg
    13501 atcgttgacg tccggagaaa tagacgccgt gcggttggtt gggccgagaa caccggcaac
    13561 gtagcgcggt ttctccgggg tgcgcgcggt ccactcatca gcacaggcgc gcgccagttt
    13621 cgccgccgca aagttgattt ccgccgacag ggattccatc tggtaatccg ccatcgcaat
    13681 ggtcgtggag ttgaaggtgt tggtttcgat gatatccgcg cccgcttcaa agtaggcgtt
    13741 gtggatagcg gcgatcactt ccggtttact gagtaccagc aggtcgttgt tgcctttgag
    13801 gtcgcatggc cagtcggcaa agcgttcacc acgaaaatcg gcttcgttca gtcgatagct
    13861 ctggatcatg gtgcccatac cgccgtccag caccagaata cgttcattta actgcgcacg
    13921 cagttgttcc actttgctgc tcacacttgc tcccgacacg ctcaacccag atttaataaa
    13981 aattcagcaa accatactgg cataaacgca tctgtggtaa aagcgaccac cacgcaacat
    14041 gagatttgtt caacattaac tcatcggatc agttcagcaa ctattgcatt agctaacaat
    14101 aaaaatgaaa atgatttcca caatacagaa aaaggagact gtcatggtcg cacccattcc
    14161 cgcgaaacgc ggcagaaaac ccgccgttgc caccgcacca gcgactggac aggttcagtc
    14221 tttaacgcgt ggcctgaaat tactggagtg gattgccgaa tccaatggca gtgtggcact
    14281 cacggaactg gcgcaacaag ccgggttacc caattccacg acccaccgcc tgctaaccac
    14341 gatgcaacag cagggtttcg tgcgtcaggt tggcgaactg ggacactggg caatcggcgc
    14401 acatgccttt atggtcggca gcagctttct ccagagccgt aatttgttag caattgttca
    14461 ccctatcctg cgcaatctaa tggaagagtc tggcgaaacg gtcaatatgg cggtgcttga
    14521 tcaaagcgat cacgaagcga ttattatcga ccaggtacag tgtacccacc tgatgcgaat
    14581 gtccgcgcct atcggcggta aattgccgat gcacgcttcc ggtgcgggta aagccttttt
    14641 agcccaactg agcgaagaac aggtgacgaa gctgctgcac cgcaaagggt tacatgccta
    14701 tacccacgca acgctggtgt ctcctgtgca tttaaaagaa gatctcgccc aaacgcgcaa
    14761 acggggttat tcatttgacg atgaggaaca tgcgctgggg ctacgttgcc ttgcagcgtg
    14821 tattttcgat gagcaccgcg aaccgtttgc cgcaatttca atttccggac cgatttcacg
    14881 tattaccgat gaccgcgtga ccgagtttgg cgcaatggtg attaaagcgg cgaaggaagt
    14941 gacgctggcg tacggtggaa tgcgctgact tttttctggc gggcagaggc aatattctgc
    15001 ccgtcatatc tgagtggcaa tagaataagg gtgtctgtta atcgcattga cgccaaaata
    15061 acttattgtc atacacttcg cacactttta ttaactcagc attattttta aacatcaaac
    15121 cacttaatta ttaacaaaat cgcaagatct cattacgccg aaacttttac
//
LOCUS       DAJOWB010000111        14994 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJOWB010000111 DAJOWB010000000
VERSION     DAJOWB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.111"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(109..2388)
                     /gene="maeB"
                     /locus_tag="OHO85_003715"
     CDS             complement(109..2388)
                     /gene="maeB"
                     /locus_tag="OHO85_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCQ2673034.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2677..3627
                     /gene="tal"
                     /locus_tag="OHO85_003716"
     CDS             2677..3627
                     /gene="tal"
                     /locus_tag="OHO85_003716"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCQ2673035.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3647..5650
                     /gene="tkt"
                     /locus_tag="OHO85_003717"
     CDS             3647..5650
                     /gene="tkt"
                     /locus_tag="OHO85_003717"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCQ2673036.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5746..6789)
                     /locus_tag="OHO85_003718"
     CDS             complement(5746..6789)
                     /locus_tag="OHO85_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCQ2673037.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6915..7490)
                     /gene="nudK"
                     /locus_tag="OHO85_003719"
     CDS             complement(6915..7490)
                     /gene="nudK"
                     /locus_tag="OHO85_003719"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCQ2673038.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(7558..9537)
                     /gene="aegA"
                     /locus_tag="OHO85_003720"
     CDS             complement(7558..9537)
                     /gene="aegA"
                     /locus_tag="OHO85_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCQ2673039.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9743..11443
                     /gene="narQ"
                     /locus_tag="OHO85_003721"
     CDS             9743..11443
                     /gene="narQ"
                     /locus_tag="OHO85_003721"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCQ2673040.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11607..14720
                     /gene="acrD"
                     /locus_tag="OHO85_003722"
     CDS             11607..14720
                     /gene="acrD"
                     /locus_tag="OHO85_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCQ2673041.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14819..14878)
                     /gene="ypfM"
                     /locus_tag="OHO85_003723"
     CDS             complement(14819..14878)
                     /gene="ypfM"
                     /locus_tag="OHO85_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCQ2673042.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3523 a         3981 c         3907 g         3583 t
ORIGIN      
        1 agactgcttt ttttattatt cgcaaactca gtaataagct ttactaatat attgcagcca
       61 aaccgcttac cctgaatatt cagggtaagc gtgagagtta aaaaagaatt acagcggttg
      121 ggtttgcgct tcaaccacgg ccagcgccac catgttgacg atacgacgca ccgatgcgat
      181 cggcgttaac acgtgaaccg gtttcgccac gcccatcagc accggaccga cagtcacacc
      241 ttccgagctg gaaacgcgca gtaagttgta actaatgcgg gcagcttcca tgttcggcat
      301 caccagaata ttggcagaac ctttcaaagg gctgtccggc atacggtcgt tgcgaatcgc
      361 ttccaccagc gctgcatcgc cgtgcatttc accatcaatc atcagatctg gcgcacgatc
      421 cctgaccagt tccagcgcct gacgcatttt gctcgacgac gggcagtcag aagaaccaaa
      481 gttggagtgc gacaacagag caacgcgcgg ctcaatacca aaacgacgga cagtttctgc
      541 cgccatcaag gtgatctccg ccagctcttc tgcgtctggt tcgtcattga cgtaggtatc
      601 ggcaataaag gtgttaccac tcggcagcag cagcgcgttc attgcacctg cggtgtgaac
      661 gccatcgcga taaccaaaga catttttcac cacgctaaaa tgttcatgat aatcgcccac
      721 cgtaccgcag atcattgcat cggcttcccc acgctgaacc atgatcgcgc cgatcactgt
      781 cgggttactg atcagcgccc gctgcgcctg ttcctgagtg acaccgcgac gcttcatgat
      841 ctggaagtat tcggtccagt actctttaaa gcgcggatcg gattcgttat tgacgatctc
      901 aaaatcaacg cccgctttga tctgcaagcc cagtttctga atgcgcattt cgatcacgtt
      961 cggacgaccg ataaggatcg gtttcgccag tcccagcgtt accagttcct gagtggcatg
     1021 cagaacgcgc gcctcttccc cttctggcag aacaacgcgc ttcggcgctt tgcgagcctg
     1081 ggagaaaatc ggcttcataa acaggttggt tttgtaaacg aactcagtca gcttgtcgat
     1141 gtagacgtcg aaatcagcaa tcggacgcgt cgccacgccc gactccatcg cggctttagc
     1201 gaccgcagga gcgatcttaa cgatcaagcg cggatcaaac ggttttggaa tgatgtattc
     1261 cggaccaaag ctcagatcct gatcgccata cgctgaagcc accacttcgc tctgttcggc
     1321 gtgggccagt tccgcaatcg cgcgtaccgc cgccagtttc atctcttcat tgatagccgt
     1381 tgcgccaaca tccagcgcgc cacggaagat aaacgggaag caaaggacgt tgttgacctg
     1441 gtttggatag tcagaacgac cggtgcaaat gatggcatcc ggacgcactt ctttcgccag
     1501 cggcggcaga atttccggtt ccgggttcgc cagcgccagg atcattggcg cacgagccat
     1561 tttcttcacc atttcctggg tcagcacttt cgggccggaa catccaagga aaatatccgc
     1621 gccttcaatc acatcatcga gggtacgttt gccgtcatcc accaccgcat atgcggcttt
     1681 ggtttccgcc atgtttggct cacggccctg atagataacg ccttttgaat cgcaaaccac
     1741 gatgttatgt ttttgcagac ccagcgctac cagcaggttc atacaggcga ttgctgcggc
     1801 acccgcgccg gaaaccacca tccgcacgtc ggagatgttt ttctccacca cgcgcaagcc
     1861 gttgaggatg gcggcagtgc tgatgattgc cgtgccgtgc tgatcgtcgt ggaataccgg
     1921 aatattcatc cgctcgcgca gtttctgttc aatatagaaa cattctggcg ctttaatgtc
     1981 ttcgaggttg attccgccga aggtcggttc gagcgcagcg acaacttcaa taaatttgtc
     2041 cgggtcgagt tcatcaactt caatgtcaaa tacatcaatc ccggcgaatt tcttaaacag
     2101 aacgcccttg ccttccatca ccggtttgcc agccagcgcg ccaatgttgc ctaaccccag
     2161 caccgccgta ccgttagaga tcaccgccac caggttaccg cgcgcggtat atttgtaggc
     2221 ttttaacggg tctttttcga tttcaagaca aggtgcggca acgcctggtg agtaggccag
     2281 cgccagatcg cgctgcgttg ccagaggctt ggttggagaa acctggattt tccctggaac
     2341 tggaaattca tggaaatcaa gtgcactttg ttttaactgg tcatccattt ggttgttcct
     2401 ttcacgtaac gttcacaaat aaagtgtgtg ggcaacagcc cctgcccaca acgtggcgca
     2461 cattattacc ctgccggagt ctacagactt tgagcaagtc caaactctca ccattaatat
     2521 aatgttttgg taataatcct ataacactga tgttacctgc ttaatccagc aataccatgc
     2581 ctgtctgcta tgcttttttg atgcgtttag cgaaatttct cagaagtgtg aattaacgca
     2641 ctcatctaac actttacttt tcaaggagta tttcctatga acgagttaga cggcatcaaa
     2701 cagttcacca ctgtcgtggc agacagcggc gatattgagt ccattcgcca ttatcatccc
     2761 caggatgcca ccaccaatcc ttcgctgtta ctcaaagctg ccggattatc acaatatgag
     2821 catttaatag acgatgctat cgcctggggt aaaaaaaatg gcaagaccca ggaacaacag
     2881 gtggtcgcag cgtgtgacaa actggcagtc aatttcggtg ctgaaattct caaaatcgta
     2941 cccggtcgcg tgtcaacaga agttgatgca cgcctctctt ttgataaaga aaagagtatt
     3001 gagaaggcgc gccatctggt ggacttgtat cagcaacaag gcgttgagaa atcacgcatt
     3061 ttgatcaaac tggcttcgac ctgggaagga attcgcgcgg ccgaagagct ggaaaaagaa
     3121 ggtattaact gcaacctgac gctgctgttt tcttttgccc aggcgcgggc ctgtgcagaa
     3181 gcgggcgttt ttctgatttc gccgtttgtc gggcgtattt atgactggta tcaggcacgc
     3241 aagccgatgg acccgtatgt ggtggaagaa gatccgggcg ttaaatcggt gcgcaatatc
     3301 tacgactact ataagcaaca ccactatgaa accattgtga tgggcgcgag cttccgtcgc
     3361 accgaacaaa tcctcgcctt aaccggctgc gatcgactga ctatcgcacc gaatttgctg
     3421 aaggagctgc aggaaaaagt ttcgccagtg gtacgtaaat taatcccgcc ttctcagacg
     3481 ttcccacgcc cagcacccat gagcgaagcg gagttccgtt gggagcacaa tcaggatgcg
     3541 atggcggtag aaaaactgtc tgaaggcatt cgtctattcg ccgttgatca acgcaaactg
     3601 gaagatcttc ttgccgccaa actataaacc agccacggag tgttatatgt cccgaaaaga
     3661 ccttgccaat gcgattcgcg cactcagtat ggatgcggta caaaaagcca attctggtca
     3721 tcccggcgcg ccgatgggca tggctgatat tgccgaagtg ctgtggaacg attttcttaa
     3781 acataatcct accgacccaa cctggtatga tcgcgaccgc tttattcttt ccaacggtca
     3841 cgcgtcgatg ctgctctaca gtttgctaca tctgaccggt tacgacctgc cgctggaaga
     3901 actgaagaac ttccgtcagt tgcattcgaa aaccccaggc cacccggaga ttggctatac
     3961 gccaggcgtt gaaaccacca ccggcccgct tggacaaggt ttggcgaacg ccgtcgggct
     4021 ggcgatagcg gaacgtacac tggcggcgca gtttaaccag ccagaccatg agatcgtcga
     4081 tcacttcacc tatgtgttta tgggcgacgg ctgcctgatg gaaggtattt cccacgaagt
     4141 ctgttcgctg gcaggcacgc tgggactggg caagctgatt ggtttttacg atcacaacgg
     4201 tatttccatc gacggtgaaa cagaaggctg gtttaccgac gatacggcaa aacgttttga
     4261 agcctatcac tggcatgtga tccatgaaat cgacggtcac gatccgcagg cggtgaagga
     4321 agcgatcctt gaagcgcaaa gcgtgaaaga taagccgtcg ctgattatct gccgtacggt
     4381 gattggcttt ggttcgccga ataaagcagg taaggaagag gcgcacggcg caccactggg
     4441 ggaagaagaa gtggcgctgg cacggcaaaa actgggctgg caccatccgc catttgagat
     4501 ccctaaaaag atttatcacg cctgggatgc ccgtgaaaaa ggcgaaaaag cgcagcagag
     4561 ctggaatgag aagtttgccg cctataaaaa ggctcatccg caactggcag aagagtttac
     4621 ccgacggatg agcggtggtt taccgaagga ctgggagaaa acgactcaga aatatatcaa
     4681 tgagttacag gcaaatccgg cgaaaatcgc tacccgtaag gcttcgcaaa atacgcttaa
     4741 cgcttacggg ccgatgctgc ctgagttgct cggcggttcg gcggatctgg ctcccagcaa
     4801 cctgaccatc tggaaaggtt ctgtttcgct gaaggaagat ccagcgggca actacattca
     4861 ctacggggtg cgtgaatttg gcatgaccgc tatcgccaac ggcatcgcgc accacggcgg
     4921 ctttgtgccg tataccgcga cgttcctgat gtttgttgaa tacgcccgta acgccgcgcg
     4981 gatggcggca ctgatgaaag cgcggcagat tatggtttat acccacgact caattggcct
     5041 gggcgaagat ggtccgacgc accaggctgt tgagcaactg gccagcctgc gcttaacgcc
     5101 aaatttcagc acatggcgac cgtgcgatca ggtggaagcg gcggtgggct ggaagctggc
     5161 ggttgagcgc cacaacggac cgacggcact gatcctctca aggcagaatc tggcccaggt
     5221 ggaacgtacg ccggatcagg ttaaagagat tgctcgtggc ggttatgtgc tgaaagacag
     5281 cggcggtaag ccagatatta ttctgattgc caccggttca gagatggaaa ttaccctgca
     5341 agcggcagag aaattagcag gagaaggtcg caatgtacgc gtagtttccc tgccctcgac
     5401 cgatattttc gacgcccagg atgaggaata tcgggagtcg gtgttgcctt ctaacgttgc
     5461 ggctcgcgtg gcggtggaag caggtattgc cgattactgg tacaagtatg ttggtctgaa
     5521 aggggcaatt gtcgggatga cgggttacgg ggaatctgct ccggcggata agctgttccc
     5581 gttctttggc tttaccgccg agaatattgt ggcaaaagcg cataaggtgc tgggagtgaa
     5641 aggtgcctga tggtgatcgc cggatgctga ttgccggatg cgacgctgac gcgtcttatc
     5701 cggcctacag cgtcttatcc ggcctacatg tccccgcaat tttgtttaac gggtgatcca
     5761 caacgtgggc caggcgtctg gcccatgcca gttatcgcag gtgggttctg cggcataacg
     5821 caccaggcga aaacgctgac cgtcaaagcg ccagcgcgcc tgaatgccac aatcgcttaa
     5881 tccgcgccct ttcgctaagg tcaccagttc acgcgatttc tcatcaaatg ttgcgttcat
     5941 cagttccagt tcattcgtct cctgaccgtt gttgaacggc aaacgcaacc gaaccgggcg
     6001 cgaggctagt ggctttttac gcgacacaat ccatgccaaa tcaatggtgt tataggcccc
     6061 tgcctcacag ctaatcatca tcagcgcttt atcatcagtc agcgcagtga cattcacctc
     6121 acgacgtaac ggatcaagcg agcagcgcag accattcatc cgccaatttc cataatccag
     6181 caaatcgttg cgttcttcga gtgagagtgg cgtcggcgtt gggttaacca ccgcgacctc
     6241 tttcagcgca ggcgcgggcg gtacgctgag cggcggttcg tcccctttct tgatccacgc
     6301 ggtttcactg ccaacgcgct tttgctgagc atcaataaac aacaacgccg cttttaagcc
     6361 actcagagaa atggtctgat tgccgtcgcg taaggtgatc gccttccctt cctgaatcat
     6421 ctgcaaaaac gcggtgatgg ttgccgtatc atcggttact aataaccatg gtgaaatccg
     6481 ccacttgtca ccacttaacg ccaacggctc accatctaac agcagccgtg gcgctatctc
     6541 tccttcagac gcatccggcg acttcaaccc gccgcgctca atacgtaaaa cggcatcggt
     6601 atgcgccccg gcgctgcggc tcagggtcat caccagtcca ttatgatcgc ccgtattgcg
     6661 cgccacgcag aagttttggt tattacaggt gacctgccag tcggaaaacg cccgttgcgc
     6721 tggtgccgcc cacaccaaag acgtcggcaa caaagcgaaa aaaaagagaa ggaaaatgcg
     6781 atagcgcatg gacggtacga ccccagaagt tcaaacaaac agcacaagtc gtatcttcgt
     6841 tgcgcaggta gcgcggctca atcagatttg tcggattgag ccaataataa acaacgaaat
     6901 attattgtta tttttcagtc cattaaatgt gacgtttgca aatagttaag caataacacc
     6961 gtcttaccgt cacgtatctc gccggttttg atcatctcca gcgcctggct gaacggcagc
     7021 tcgagcactt caatatcttc atcttcaaca ccgccaccgg cgttagcgcg ctgattgtca
     7081 ctgtattcgg cgataaaaaa gtggattagc tcagtcacac cgccaggcga catatacagt
     7141 tcaaataatt tgcgcacttc gcccacttca tagcccgtct cttcaatcgc ttctttacga
     7201 atgcacactt ccggttcgtc gttatccagc agcccggcgc aggtttcaat cagctgcccg
     7261 ctttcattgc cattaaccca ggtagcgaca cggaactgac gaatcagaac cacggtcttt
     7321 ttcttcgcgt tgtacaggag gatcgtcgca ccattaccgc gatcgtaaac ttcacgttta
     7381 tggcggataa cttcgccatc tttgcgcgtg agatcgtaag taatgttgtg cagggtgaaa
     7441 tagttatcgg agagaatttt gtctttaatg agggtgattt gttgcgtcat agcgactcca
     7501 cagcgcgaaa tgaacaatta tcttacgctg tgaagtcggg tttgtctgcg caggctatca
     7561 gtgagatttg actgatttta cccccagcca gtcaataatg ccctgtgccg catgacgtcc
     7621 ttctgccatt gcggtaacca ccagatccgc accacgcacg gcgtcgccac cggcgaagat
     7681 tttcggattg gtggtctggt aacggtactg gctttccaca tccgcgatga tgcggcccca
     7741 tttgtctacc gttacaccgt gcgactccag ccacggcatc ccgtgtggat tgaagccaaa
     7801 cgccataatc accgcatcgg ctggcatgac aaattcactg ccctcaatcg gtacaggtcg
     7861 ccgacgcccc tgggcatccg gctcgccaag acgcgtgcgc aggaaacgaa ttccgcaaac
     7921 gtgaccttgt tcattcagct caagcgccac cggctggacg ttaaattcga agttggcccc
     7981 ctcttcgcgg gcgttcttca cctctttctt cgagcctggc atgttagctt catcacgacg
     8041 ataagcgcag gtgacgttac tcgcgccgtg gcgcagcgca gtacgcacac agtccatcgc
     8101 ggtgtcgccg ccgcccagta ccacgacgtt aagtccggcg gtattgataa acggctcttc
     8161 cggtagctct tcgagcccca tcacctgttt agtgttggca atgaggaacg gcagcgcgtc
     8221 ataaacgccc ggcgcatctt cattgggtaa acccgctttc atggaacggt aagtgcctac
     8281 gccaacgaag accgcgtcgt attgttccaa aagcgaatcc aaagagacat ctttacccac
     8341 ttcacaattg agttcgaagt gaatccccat cgcgctgaag atttcccgac ggcgtgccaa
     8401 cagggattta tccagtttga aagaaggaat gccgaaagtg agcaagccac caatttctgg
     8461 atggcgatcg tacaccgtca ccgccacgcc attgcgggtc agaacatccg cacaggccag
     8521 ccctgccggg cctgccccga taatcgccac ccgcttgtcc actttggtga catgacttaa
     8581 gtcaggacgc caacctttcg ccaacgcctg atctgaaata tagcgttcaa tgttgccgat
     8641 agttacagca ccgtgctcat cgcgaatagt acaggcacct tcacacaaac ggtcttgcgg
     8701 acaaacgcgt ccggtaattt ccggcagggt gttggtctgg tgagaaagct cgacggcggc
     8761 gtcgatgttt ccggctttca ccagttcaat ccactgcgga atatggttat gcagcgggca
     8821 ggtccattca caaacgctgt gctcgccgca cttaaggcag cgcgaggctt cccgttgtgc
     8881 ctggtcggcg cgaaatggca gataaatttc atcaaaaccg gttttgcgcg cttcaatcgc
     8941 cagtttatcc ggctcgccac gcgcgggcgt tgcctgcatt tgttcgactt tactcattac
     9001 cggcatttct tgcgccgcgg tactggcatg ccacggttga tgttcctgac gcgcggtgcg
     9061 caagcggcgg gatttcgcca tgccggagag tgcgacgtca gtgaccagtt gcagcgcgtc
     9121 cgccgggcaa ttctcaacac aggcaggacc gttttcgcgc cccgcacaaa ggtcgcattt
     9181 atgcgccgtg gcttttactt ttcctgccgc gacgggtgtc aggacgattt gcatcgtacc
     9241 aaaaggacag gccaccacgc aggatttaca gccaatacac ttttgctgat tgacctgaat
     9301 gctgtcatca acgtggctga ttgcgccatt agggcagcta cgggcgcagg gcgcatcttc
     9361 acaatggtga caggtcactg cactacgttg ctgttgatgt ttgataaccg taattcgggg
     9421 atgaaaatgg tgttggctca ggacatgttg ctcatcattg tgagccatga cacaggcgat
     9481 ttcacaagca tgacaaccca gacattgctg actgttggcc ataataaaac gattcataac
     9541 gaccttcttt tttggttgta aaaaccttat tctttatatg agtgttgtta ttacccgcct
     9601 tacaggggaa tcggcaatgt tcatgtgccc agaataagta actatttcgc tataaactgt
     9661 ggcagatcaa ataatcccat cactgactaa attgtgtttc agctaactgg aacattaatg
     9721 attttttgtg gagaagacgc gtgtgattgt taaacgaccc gtctcggcca gtctggcccg
     9781 ggcctttttt tacattgtgc tgctgtcgat tctttccacg ggtatcgctc tgctaactct
     9841 ggcgagcagt ttgcgcgacg ctgaggctat caatattgcc ggatcgctac gtatgcagag
     9901 ttaccgcctg ggctacgact tgcaaagtgg cagtccacaa ctcaatgcac atcgccagtt
     9961 gtttcagcag gcactgcatt caccggtatt aaccaatctc aacgtctggt atgtgccaga
    10021 agcagtaaaa actcgctatg cgcatctgaa tgccaactgg ctggagatga ataatcggct
    10081 cagcaagggc gatttgccgt ggtatcaggc caatattaat aattatgtta atcagataga
    10141 cctgttcgtg ctggctttac agcactacgc tgaacgcaaa atgctgctgg tggtggcgat
    10201 ttccctggct ggcggtatcg gtattttcac gctggtcttt tttactttgc gccgtatacg
    10261 ccatcaggtg gttgccccgc tgaatcagct ggttaccgcc agtcaacgta ttgaacacgg
    10321 gcagttcgac tcgccgccgc tggataccag tctgccgaat gagcttggtc tgcttgcaaa
    10381 aacctttaac cagatgtcga gcgagctgca taaattgtac ctttcgctgg aggcgtcagt
    10441 agaagaaaag acccgcgatc tccacgaggc caagcgtcgt ctggaggtgt tgtatcaatg
    10501 ttcccaggcg ctgaacacca gccagattga tgtgcattgt ttccgccata ttttgcagat
    10561 tgttcgcgat aatgaagcgg ctgaatatct ggagttaaat gtcggtgaaa actggcggat
    10621 tagcgaaggg caaccaaacc cggaattgcc gatgcagatt ttaccggtga caatgcaaga
    10681 gacggtttac ggcgaactgc actggcaaaa tagtcacgtt tcatcatcag aaccgctgct
    10741 taacagcgtt tcgtcgatgc tgggacgcgg tttgtacttt aatcaggcgc agaagcattt
    10801 tcagcagtta ttgttgatgg aagaacgtgc aaccatcgcc cgcgaattgc acgactcgct
    10861 ggctcaggta ctttcttact tacgtatcca gttgacgtta ctgaagcgtt cgataccgga
    10921 agataacgcc accgcacaaa gtatcatggc cgatttttcc caggcgttga atgatgctta
    10981 tcggcagtta cgcgagctgt tgactacttt ccgcctgacg ctgcagcagg cggatctccc
    11041 ctccgcgttg agggaaatgc tggatacgtt acaaaatcaa accagcgcca aactgaccct
    11101 cgactgccgt ctgccaaccc tggcgctgga tgcgcaaatg caggtgcatt tgttgcaaat
    11161 tattcgcgaa gcggtgctga atgcgatgaa gcacgccaac gccagcgaaa tcgccgtcag
    11221 ttgcgtcacc gcgccggacg gcaatcacac ggtttatatc cgtgataacg ggattggtat
    11281 cggtgaaccg aaagaacccg aaggtcatta tggtctgaat atcatgcgcg aacgcgcgga
    11341 acggctgggt gggacgctga ctttttcaca accttccggc ggcggcacgt tagtgagtat
    11401 tagctttcgc tccgcggagg gtgaggaaag tcagctgatg taatgcctcc tactgaccaa
    11461 agaatacttg cacttaaggt tcagtataaa agggcatgat aatttacatt aactcctttt
    11521 tttctccacg attggctcgt accttgccgc tacagtgaag caagtcaagc ctacaacgat
    11581 acgcagaaac acgaggtcct cttttaatgg cgaatttctt tattgatcgc cccatttttg
    11641 cctgggtgct ggcaatcctg ttgtgtctga caggtaccct ggcgattttc tcattgcccg
    11701 ttgaacaata ccccgatctc gcgccaccga atgtgcgagt gaccgctaac tatcccggcg
    11761 catcggccca gacgctggaa aacaccgtga cccaggttat cgagcaaaat atgaccggcc
    11821 tcgataatct catgtatatg tcatctcaga gcagtggcac cggtcaggca tctgtcactt
    11881 taagttttaa agcaggcacc gatccggacg aagccgtgca gcaagtacaa aaccagctgc
    11941 aatcagccat gcgaaagtta ccgcaggcgg tgcaaaatca gggcgtgacg gtgcgtaaaa
    12001 ccggcgatac caatattctg accattgctt tcgtctctac cgatggttcg atggataaac
    12061 aggatattgc cgattatgtt gccagtaata ttcaggaccc gttaagccgc gtgaatggcg
    12121 tcggggatat cgatgcctat ggttcgcaat attccatgcg tatctggctg gacccggcga
    12181 aactcaacag tttccagatg acggctaaag atgtcactga tgccattgag tcacagaacg
    12241 cgcagattgc ggttgggcaa cttggtggta caccttccgt cgataagcag gcgctcaacg
    12301 ccaccattaa cgcccagtca ctgctgcaaa caccagaaca gttccgcgat atcaccttgc
    12361 gggtgaatca agacggctca gaggtaaggc tgggcgatgt cgccaccgtc gaaatggggg
    12421 cggagaaata cgattatctt agccgcttca atggtaagcc agcctccggg ctgggggtaa
    12481 aactggcctc cggcgcaaac gaaatggcga cagcggagct ggtgctcaat cgtctcgacg
    12541 agctggcgca gtacttcccg cacggactgg aatacaaggt ggcgtatgaa accacctcgt
    12601 ttgttaaagc ctccattgaa gacgtggtga aaacgctgct ggaagctatc gctctggttt
    12661 tcctcgttat gtatctgttc ctgcaaaact tccgcgccac gctgataccc actatcgccg
    12721 tgccggtggt gttgatggga accttctccg tactttacgc cttcggttac agcgtcaaca
    12781 ccttaaccat gttcgcgatg gtgctggcga tcggtctgct ggtggatgac gccatcgtgg
    12841 tggtggaaaa cgtcgaacgt attatgagtg aggaaggact cactcctcgc gaagccacac
    12901 gtaaatcgat ggggcagatc cagggggcac tggtcgggat tgcgatggtt ctttcggcgg
    12961 tatttgtacc aatggccttc ttcggcggca ccaccggtgc catctatcgc cagttctcta
    13021 ttaccattgt tgcggcgatg gtgctgtcag tactggtagc gatgatcctc actccggctc
    13081 tgtgtgccac actacttaag ccactgaaaa aaggtgagca tcacgggcaa aaaggctttt
    13141 ttgcctggtt taaccagatg tttaaccgca acgccgaacg ctacgaaaaa ggggtggcga
    13201 aaattctcca ccgtagcctg cgctggattg tgatttatgt cctgctgctt ggcggcatgg
    13261 tgttcctgtt cctgcgtttg ccgacgtcgt tcttaccgct ggaagaccgt ggcatgttta
    13321 ctacctcggt acaattgccc agcggttcaa cccaacaaca gaccctgaaa gtcgttgagc
    13381 aaatcgagaa atattacttc acccatgaaa aagacaacat catgtcggtg tttgccactg
    13441 ttggttctgg ccccgggggt aacgggcaaa acgtggcgcg aatgtttatc cgcctgaaag
    13501 actggagcga acgcgacagt aagaccggca cctcgtttgc cattatcgag cgtgcaacaa
    13561 aggcgtttaa caagattaaa gaagctcgcg ttatcgccag cagcccgcca gcaattagcg
    13621 gtcttggtag ttctgcgggt tttgatatgg agttgcagga ccacgctgga gcaggtcacg
    13681 atgcgctgat ggcagcacgt aaccagttgc tggcgctggc ggcggaaaac ccggagctaa
    13741 cccgtgtgcg ccataacggc ctcgacgaca gtccgcagtt gcagattgat atcgaccagc
    13801 gtaaagctca ggcgctgggc gttgatatcg acgatattaa cgacacactg caaaccgcct
    13861 ggggttcgag ctatgttaat gactttatgg atcgcggtcg cgtgaagaaa gtctatgtgc
    13921 aggcagctgc gccgtatcgc atgctgccag atgacatcaa tctctggtat gtccgaaata
    13981 aagatggcgg catggtgccc ttctctgctt tcgcgacctc acgctgggag acaggctcgc
    14041 cgcgtctgga acgctataac ggttattctg cggttgagat tgttggggaa gccgcaccgg
    14101 gggtcagtac cggtacggcg atggatatta tggaatcgtt agtgaagcag ttgccaaacg
    14161 gctttggtct ggagtggacg gcgatgtcgt atcaggagcg gctttccggc gcgcaggctc
    14221 cggcgctgta cgctatttcc ttgctggtgg tattcctgtg tctggcggcg ttgtatgaaa
    14281 gctggtcggt gccgttctcg gtaatgctgg tcgtgccgct gggggttatc ggcgcgctgc
    14341 tggcaacctg gatgcgcggg ctggaaaacg acgtttactt ccaggtgggc ctgttaacgg
    14401 tcattggttt atcggcgaaa aacgccatcc tgatcgtcga gtttgctaac gagatgaacc
    14461 aaaaaggcca cgacctgttt gaagcgacgc tccacgcctg ccgtcagcgt ttacgcccga
    14521 ttctgatgac ctcgctggca tttatcttcg gcgtattgcc aatggcaacc agcacgggtg
    14581 ccggttccgg tggtcagcat gcggtgggta ctggcgtaat gggcgggatg atttcggcca
    14641 ctattctggc tatttacttc gtgccgctgt tctttgtact ggtgcgccgc cgcttcccgc
    14701 tgaagccgcg cccggaataa gcaataaaaa aggcgacatg ctaatgtgtc gcctttttca
    14761 actttccgat taagaacctg ctcagcgggt tcttgctgtt tgtactttgt ctcaggaatt
    14821 acttacgaag cataacttcg ataaagtctt tccagttccc cagttcacgt tcaatcataa
    14881 caacctctct tataattatg ggtattctac ggaaacaata taccgtggtg aagctaattt
    14941 actcgattgc tgcgatgact acctccgggg gacaaacctt atgtaaatac tatg
//
LOCUS       DAJOWB010000112        14990 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJOWB010000112 DAJOWB010000000
VERSION     DAJOWB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.112"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(28..492)
                     /locus_tag="OHO85_003724"
     CDS             complement(28..492)
                     /locus_tag="OHO85_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCQ2673043.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IHTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAAINPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRGQM"
     gene            612..1421
                     /gene="proC"
                     /locus_tag="OHO85_003725"
     CDS             612..1421
                     /gene="proC"
                     /locus_tag="OHO85_003725"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCQ2673044.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(1438..2553)
                     /gene="adrA"
                     /locus_tag="OHO85_003726"
                     /gene_synonym="dgcC"
     CDS             complement(1438..2553)
                     /gene="adrA"
                     /locus_tag="OHO85_003726"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCQ2673045.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(2655..2975)
                     /gene="psiF"
                     /locus_tag="OHO85_003727"
     CDS             complement(2655..2975)
                     /gene="psiF"
                     /locus_tag="OHO85_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCQ2673046.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(3094..4509)
                     /gene="phoA"
                     /locus_tag="OHO85_003728"
     CDS             complement(3094..4509)
                     /gene="phoA"
                     /locus_tag="OHO85_003728"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCQ2673047.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWQGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(4610..4870)
                     /gene="iraP"
                     /locus_tag="OHO85_003729"
     CDS             complement(4610..4870)
                     /gene="iraP"
                     /locus_tag="OHO85_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCQ2673048.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(5052..5255)
                     /locus_tag="OHO85_003730"
     CDS             complement(5052..5255)
                     /locus_tag="OHO85_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673049.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            5333..6427
                     /gene="ddlA"
                     /locus_tag="OHO85_003731"
     CDS             5333..6427
                     /gene="ddlA"
                     /locus_tag="OHO85_003731"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCQ2673050.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(6451..6663)
                     /locus_tag="OHO85_003732"
     CDS             complement(6451..6663)
                     /locus_tag="OHO85_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCQ2673051.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            6923..7231
                     /locus_tag="OHO85_003733"
     CDS             6923..7231
                     /locus_tag="OHO85_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCQ2673052.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(7290..8384)
                     /gene="yaiW"
                     /locus_tag="OHO85_003734"
     CDS             complement(7290..8384)
                     /gene="yaiW"
                     /locus_tag="OHO85_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCQ2673053.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVGSVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPPFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(8397..9617)
                     /gene="sbmA"
                     /locus_tag="OHO85_003735"
     CDS             complement(8397..9617)
                     /gene="sbmA"
                     /locus_tag="OHO85_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCQ2673054.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            9969..11126
                     /gene="ampH"
                     /locus_tag="OHO85_003736"
     CDS             9969..11126
                     /gene="ampH"
                     /locus_tag="OHO85_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCQ2673055.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKQLVIPAS"
     gene            complement(11127..11750)
                     /gene="iprA"
                     /locus_tag="OHO85_003737"
     CDS             complement(11127..11750)
                     /gene="iprA"
                     /locus_tag="OHO85_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCQ2673056.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(11838..14789)
                     /locus_tag="OHO85_003738"
     CDS             complement(11838..14789)
                     /locus_tag="OHO85_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCQ2673057.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAANYDTWGYHDNTTTDLV
                     WPYDTDNDGIADAGGMDPMGSDGNYVTYNGFVYYNNANGDFDTVFKGDTVNGTISTYY
                     LNHDYASGSVNQVDISNSVIHGSITSELPFGYYSITPEGYNGYTFNNGTTTDLDYNWY
                     DGDVFTLNVSNSTIDDDYEALYFTDTHLDGDKSKSTNETFYTALGVAVNLDVESNINI
                     TNNSRVAGIALSEGNTSNTTYTSEYHQWDNNINVSNSTVTSGSQTPLEESDGFFGKSA
                     EPSDYAGNGGQNDVALSFTDNAGSNYSMKNNVNFDHSTLLGDVEFTSHWNNDGVFFYS
                     TGHDSNGDGVLDTNGGWVDDAQNVDELNITLNNGSKWVGSANMSAEVIAPADMYDVAP
                     NSLTPGATIEANDWGRIIDNKVFQSGVFNVALNNSSEWNTVNSSVIDTLAVNNGSQVN
                     VTDSSLVSDTIGLTNGSSLNIGANGVVATDHLTVDSYSTVNLTESTGWNNYSNLYTNT
                     ITVTNGGVLDVNVDQFDTEAFRTDKLELTSGNIADHNGNVISGVFDINSSDYVLNADL
                     VNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDVDNGTELDNSSVDNVVAATGNYKVR
                     IDNATGAGTIADYKDKELIRVNDANTNATFSAANKADLGAYTYQAEQRGNTVVLQQME
                     LTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGT
                     INYDQDVNGIMVGVDTKVDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSA
                     HFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYV
                     TPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKL
                     AYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQD
                     WSANVGVKYTW"
BASE COUNT         3768 a         3798 c         3569 g         3855 t
ORIGIN      
        1 aatcaagaat gaagtgtaaa taataaatta catttggccg cgactacgtt gcacttccag
       61 ccaccatttc tccagctctg cagcaaaggc ctggcggtca cgttgtgaaa gactatctgg
      121 tccgccggtc tggatcccac tggcacgtaa ggtatccata aaatcgcgca tcgtcaggcg
      181 ctcacgaatg gtcgctggcg tgtaacgttc gccgcgcgga ttaatcgccg cagcgccttt
      241 ctcgatggct tcagcagcca aaggtatatc tgcggtgatc accaaatcgc ccgcttcaca
      301 ctgccggaca atttcgttat cggcaacgtc gaaacctgcc gcgacgcgca gcgtatgaat
      361 aaatcgcgat ggcggcacgc gtaaactctg gtttgctacc agtaccagcg gcatctgcat
      421 acgttccgcc gcgcgataca aaatctcttt aattacattg ggacacgcgt cggcatccac
      481 ccaaattgtc ataaagtcat cctttgttgg gtaatcctct attgtgtcgc gcttttgcct
      541 tccggcatag ttctgtttat gcttctgcca gcgattatca aaacaatgaa tttcacggca
      601 ggagtgaggc aatggaaaag aaaatcggtt ttattggctg cggcaatatg ggaaaagcca
      661 ttctcggcgg tctgattgcc agcggtcagg tgcttccagg gcaaatctgg gtatataccc
      721 cctccccgga taaagtcgcc gccctgcatg accagttcgg catcaacgcc gcagaatcgg
      781 cgcaagaagt ggcgcaaatc gccgacatca tttttgctgc cgttaaacct ggcataatga
      841 ttaaagtgct tagcgaaatc acctccagcc tgaataaaga ctctctggtc gtttctattg
      901 ctgcaggtgt cacgctcgac cagcttgccc gcgcgctggg ccatgaccgg aaaattatcc
      961 gcgccatgcc gaacactccc gcgctggtta atgccgggat gacctccgta acgccaaacg
     1021 cgctggtaac ccccgaagat accgctgatg tgctgaatat tttccgctgc tttggcgaag
     1081 cggaagtaat tgctgagccg atgatccacc cggtggtcgg tgtgagcggt tcttcgccag
     1141 cctacgtatt tatgtttatc gaagcgatgg ccgacgccgc cgtgctgggc gggatgccac
     1201 gcgcccaggc gtataaattt gccgctcagg cggtaatggg ttccgcaaaa atggtgctgg
     1261 aaacgggaga acatccgggg gcactgaaag atatggtctg ctcaccggga ggcaccacca
     1321 ttgaagcggt acgcgtactg gaagagaaag gcttccgtgc cgcagtgatc gaagcgatga
     1381 cgaagtgtat ggaaaaatca gaaaaactca gcaaatcctg atgatttttg ccggacgtca
     1441 ggccgccact tcggtgcggt tacgtccggc tttctttgct ttgtaaagcg ccaaatctgc
     1501 cgatttcaac cactcgcgat agtgactcat ttgtgggttc agcggcgcaa cccccacact
     1561 aatccgtaaa gttacctgtg gcgtattcgg caagcgtaac gtgtttagcc cttcatgcac
     1621 ccgtaacatg gcggtaatgg cgctctcagc tggcgtaccg gacatgatta ccgcaaactc
     1681 atcgccgcca aaccgaccaa tcacatcgct accgcgcagg gtaatttgta actgtcgggt
     1741 aagcgccaca atcgcttcat cgcccacatc atggccccag gtatcgttga tgctcttgaa
     1801 atggtcgata tcgataatca gtaatgtcgc atcgcgatta tgccgccgac agttatcaaa
     1861 ttcattgcgt aacatagttt cccaatgacg tcggttatac acgcccgtca taccgtcgcg
     1921 ggtactcatg acctgcaacc tgcgtttatg ttccgccagt ttagtcgccg tctggtagct
     1981 gacccagcca aacagcagag gataaatgac gataatggga agggagagcc accattccag
     2041 cggcgcacta ttgaacgaca cggtgatgcc cgtcagttcg agggtgacaa ggcaggaaac
     2101 caccatcaac accagacccg cgacaaacag acgggggccg cctgccccca tcaaattcag
     2161 acacataatc atcaacatcg cggtggaagg cagcacgttt acgcccatta cgcctaccca
     2221 cattcccgct aatactgcat cggtttttaa gttgtaaatt tcccggctaa gcggatcgac
     2281 ggccctgctc gctatctgcc aggctaaatg cggccagacg aacgcccagc cgaccaacac
     2341 cagccaccac cagcccggcg gcgggtgcga aaccagcgtt gaagcaatcg gtaagaacat
     2401 gccagccagg ccaaccgcac ggggtagtct gacgcgacgg gcgaagcgca gcccggaccg
     2461 ctgatgttcg ttttgaggag ttaaaggagg ttcctcccca tgcgccgccg cttttttgaa
     2521 aaagttttca tcattcatta tttttgggaa cattcagaaa caattttccc aaattataga
     2581 gacggggcta aaggcagaag tatgaaattt cgggtgagcc actttcgtag ctcacccgtt
     2641 agcactatca ggtatcaggc ggctttcttg aggcaggcac tcataaactt attacgatca
     2701 tcacctttca gagattgttg cgttgcttga ttattacatt cgcgcatctt ttgctgctgt
     2761 ggcgtcaaac ttttttcacc aggcgcagac ttgctgttct tcaggcaatc actcatgtag
     2821 gtcttacgag catccccttt caacgcctgc gccgtcgcct gctgattaca ggaggtcata
     2881 cgctgttgtt gcggggttaa ggttctctcg gcagcgccga cggtggttaa aaaaaccagt
     2941 ccgaaaagca aggtaaccag taatgttatt ttcatagcac catccctctt catgttttaa
     3001 ccatgagcgt ttgcgcccgt gatctgccat taagtctggt tgctaacagc aaaaaaacca
     3061 cccgacagcg aaaattcacc gccgggcgcg gttttatttc agccccaggg cggctttcat
     3121 ggtgtagaag agatcggtct ggtcggtcag tccaacgaca ttggcggcat gtgggccata
     3181 cgccgcaata cgcagctgac taccggtatg ttcttgtgaa tcctcttcgg agttcccgta
     3241 actcatcacc atcactgcgc catctttggt atttagcgcc tgggtgaggc ccggcgcttt
     3301 ggtgtccggc gcaacaatct ggctggcgtg ggcgtgatca gcggtgacta tgaccagcgt
     3361 gttgccatcc tttttagcga attccagcgc ccgttgtacg gcttcgtcga gatcgaccgt
     3421 ctcgccaatt tgcccacaag gattcgcagc gtgatcctgt ttatcgattg atgcaccttc
     3481 aacttgcagg aaaaagcctt tctcattttt actcaacaat tcaatggctt tatcagtcat
     3541 ctgcgccagg gtcggtacgc tgtcattacg ttgcggatta ggcgtacagg taactgcggg
     3601 cttgtcgata ttgccgtgat acgttgcttt cggtccttgc cagcgcactg gcatattgcc
     3661 gtcagcaaac agtcctagca ggggtttttg ctggttcgct tccgtcaccg cattcagtga
     3721 ggcagcatcg ctcaccaact gataaccacg cgcctgtgcc tgttcacgca gcgtttttcc
     3781 ctgccattca ccggcggttg ccgtttcagc aaaggttttt gcgccgccgc caagcgtaac
     3841 atcggcacga gcgttaagca gctgttcggt aatcgatcct tttccgcctt tttctagcgc
     3901 gttacccgga catttttcac tggtcgcgct gggaccgtag catttgcgcg aggtcacatg
     3961 cgccaccagc gcagcgggcg tggcatcctg caactctgcg gtagaaacgt taccggtcgc
     4021 cagacctgcg gcttttgcca tttccagaat cgttgggtga tctttttcgt gaatatcgac
     4081 gcccagcgcg ccgttatagg ttttgacacc ggttgaccag gcggttgctg acgcagccga
     4141 atcggtgacg tagtccggtt tgccggtttt tttattcagc gcatagtgag tgtattgccc
     4201 ggtaagcggt aatgcatcga tacctttaaa aaagccgccc gcaccttcgg cataattacg
     4261 tgcggcagta atttccgaat cccccatccc atcgccaatc agcaaaataa tattttttgc
     4321 aggtttatcg ctaagagaat cacgcagagc ggcggtctga tcacccgtta agcggcgagc
     4381 accgccgggt gcagtaatat cgccctgagc agcccggttt tccagaacag gcatttctgg
     4441 tgtccgggct tttgtcacag gggtaaacag taacggtaag agtgccagtg caatagtgct
     4501 ttgtttcact ttattttctc catgtacaaa tacattaaaa aataaaaaca aagcgactat
     4561 aagtctcggc cgtgacaact ttatgacagc tgttgaaaag attaactttt tactgacgag
     4621 gatgcttcaa taacttcttt acgtaatcgc gcagcagctc cgtatcgtcg tcaggaatgc
     4681 tggcatcggg ctttacctcg tacagcgccc cctctacctg atcaatcaac cgctgttggt
     4741 cattttgcgc catattgcga agcattgcag tgacgataat ctccaaagct tctacctgcg
     4801 cacacagttc tttcgactct tcttcttttt gggcaagctt aaataacaac tcagcaatga
     4861 gatttttcat gtctgtattt ccttatccaa agtatggaga agttaacatt atgtttcaca
     4921 acttcatagt gtctggaagt attcttttta tgacaaagga aatactttgc gcaaatattt
     4981 tttgtttgat taccagcgaa acgtttctct tatttaaata actacccaca caaaatatag
     5041 gcttttaatt atcaaccttt attataaata aaagttatga aatatattct cttcgttaac
     5101 ttataattaa gagaaaaaaa ctcaccgcat tgccatttaa gccaggaata aattagcttc
     5161 ccgttcatca tttttgtgat cttgcgcaca tctttgagat aacgcacttt cttcaccagt
     5221 acgttgcacg ctttgtcagc agaagattgc aacaatcttt cagaaggccg cgaatccccc
     5281 gttttgtcgt ggtaatgtat gcactttgtg ttagacggga taggttttta agatggaaaa
     5341 actgcgggta ggaatcgttt ttggtggtaa atcagcggaa catgaagtgt ctctgcaatc
     5401 ggcaaaaaac attgtcgatg ccattgataa aagtcgcttc gacgttgtgc tgctgggcat
     5461 tgataaacaa gggcaatggc acgtcagcga tgccagcaat tatctgctaa atgcagacga
     5521 tcctgcccat attgcgttgc gcccttcggc gaccagcctt gcgcaggtgc caggtaaaca
     5581 tgagcatcag cttatcgacg cgcaaaacgg tcagccgttg ccgacggtgg atgtcatttt
     5641 cccgattgtc cacggtacgc tgggtgaaga tggttccttg cagggaatgc tgcgggtcgc
     5701 caatttaccg tttgtaggtt ctgatgttct ggcttcagca gcctgtatgg ataaagatgt
     5761 caccaaacgt ctgctacgcg atgccgggct gaacattgcg ccatttatta ccctgacgcg
     5821 cgctaatcgt cacaacatca gttttgccga agtggagtct aaactggggt taccactgtt
     5881 tgtaaaaccg gctaatcagg gctcttctgt tggtgttagc aaagtaacca gtgaagaaca
     5941 gtacgcaatt gccgtcgatc tggcgttcga gttcgatcat aaagtgatcg ttgagcaagg
     6001 gatcaaaggt cgtgagatcg aatgcgcagt tctgggcaac gacaatccgc aagccagcac
     6061 ctgtggcgag atcgtactca ccagcgattt ctatgcctac gacaccaagt acattgacga
     6121 agatggcgcg aaagtggtag ttccggcagc cattgcacca gagatcaacg ataagatccg
     6181 ggcgattgcc gttcaggctt atcagacgtt gggatgcgca ggcatggcgc gtgtagacgt
     6241 gtttttaacc ccagagaacg aagtggtgat caacgagatc aacacactgc ctggcttcac
     6301 caacatcagt atgtatccga agctgtggca agccagcggt ctgggttaca ccgatctgat
     6361 cacacgtttg attgagctgg cgctggagcg tcacgctgcg gataacgcat tgaaaaccac
     6421 aatgtaatat tttaacgccc gataatattc ttattgggcg ttttcagttt cttcatcgcg
     6481 acgacgaata atcaaccccg ccagccagaa actaatcacc cacgtcacca gaccgacggc
     6541 ataggtctgc caaccttttg cttcaaatcc cagtaacccc accacgccat taagaatgaa
     6601 tataaggcca atggcgaacg catagtagtg ccagtcacgg cggattttta caggcagatt
     6661 catggcagct ccgttaacga aaaacaacat ccttgcatat tctgtaacaa ccgtctgtgg
     6721 tgtagacaac actttcgcaa ttgttaataa atttaactta aagttgctgt tttgtgactc
     6781 gcaacgaaga ttcacgcttc gggaatattc ccaacatcaa tttaccatga ttctgatatt
     6841 gacaatctaa atgacctgtc agacttgcca taagccgctg gtatcacacc agaagagaag
     6901 cagatgactt ccggaggttt ttatggctga tttcaccctg tcaaaatcgc tgtttagcgg
     6961 aaaatatcgc aatgcctctt caacgcctgg caacattgcc tatgcgttgt ttgtgctgtt
     7021 ttgcttttgg gctggggcgc aattgctgaa cctgttagtg catgcgcccg gcgtctatga
     7081 gcgtttaatg caagtccagg aaacaggtcg cccacgggtg gaaattggtt taggtgtcgg
     7141 caccattttc gggctgatcc cgtttttagt aggctgcctc atttttgcag tggtggcgct
     7201 atggctgcac tggcgacatc gccgccagta agttttacgt tgtcagatgc ggcctgaaac
     7261 gccttatctg gcatgacatt cctgtttgat cactgtttca tacaacgcgc ccgccgttcg
     7321 tctacgcgct tcgcaaacca ggccgtagtc aggttgcggg tgattttcgg gctttccagt
     7381 tgaatgccag gcaacatttc gcggggtagt gatttaccgg ttttcgcttc ggcaagttga
     7441 taaactttct tgtacagcgc cgtctcctca aaagagaagc tgtcaccttt ctctagctga
     7501 cgacgaattt cgctgtcgct catccccagt tttgccgcca gtttgcgcgt tgccagttct
     7561 gttttcccgg gttctttact gtcgtaacga attaaatcac cgtccagtgc cagcttcacg
     7621 ccgctggcct tactgaccgc gttctgaaac gcggcattac ggctggcgta ccagccagcg
     7681 ttaaaatcag caaaacggta tatcggtgca ctataactgg cgggataatt cagtaaatgg
     7741 taagtaccaa accacaaccc gccgcggcga ctaaagactt cctgacgaac tgtaccgtcc
     7801 attttccacg gatacccttt ggtatgctgt tcggcaaaag caatgctgac ctgcatcggc
     7861 ccaccggtgc gcaccggatt aagcgaacca aacagcgtct gccccattgg caccatgctg
     7921 atcagatcgt caaaaatcgc gctcaattgc ttttctgtac gcaccgaatc taaccgttcg
     7981 ctataacttt tgccgtttgg cgatttgatt tttagcgcgg tatgaaccag aaaaggagga
     8041 atgtgcatcc gttcagcacg acggtcaatc tcttgccagg cgatcttgct taagcccgga
     8101 acggccggat ctgcctgata atttgactcc tgctgcgcca ccgccagcac cgagcagacg
     8161 ttttccagcg tcggtgccag cccctgactt tcaaaggtgg tggcgagatc tttcgcccag
     8221 gcatcgcggt ctttcacgct cgcaggcatc ttctgccgca cgacgctccc tacatctatc
     8281 gctttttcgc cctttttcaa cggttgcgga gcctgactac tacaccccgc taaaaccaga
     8341 accgccaaca atgacagcga agatagagga ttgacgcgcg acattacttc tcctttttag
     8401 ctcaaggtat gggttacttc ctgaattttg tcaccatcca gctcatgttc aaagctgcgc
     8461 agacgtttgt agatagacat caactcaacc agtgtggtcc atgagttaat caggtactgg
     8521 aaagcaccgc gaacctgacc aaaaacgttg gtaatctgcg tcatcaggcc gagcgtaatc
     8581 gtaccggcaa caattgacgg aaacagcaag aacaaaccga aaacgttatc gacctgcaaa
     8641 tagagaatgc gggcgatgtt gaaatacata tagtgaaaat agaggcgaaa atagtttttc
     8701 cgtacggcgc taaacagctc gcgtaccgta ggcggcgtcg cgcgcgtggc atcatcttca
     8761 ccataaacca gctctttacg gtaggcagcc tctacacgct ggtttttaaa ctccagcccc
     8821 ggcagtttga tccctaccac tgccagcaat ccggtcccca tcagcgacca gacgatagcg
     8881 gcaatcacca gaccatacgg aatgtgcccg ataatcggca gctccggcac atgcgcggag
     8941 agcgttacca gtaccggcag gaaggcgatt aacgtcatga tggcgttgat aaaactgacg
     9001 cccatattct ccagcgttga agcaaaacgc atggtgtctt cctgcacacg ctgtgcggca
     9061 ccttcgatat gacgcagttg ttgccagttc gccatgtaat attcgttcat cgccgtacgc
     9121 cagcggaaca cgtagtgact gacaaagaag ttattcagca cactgatcac taccgcgatc
     9181 agcgcaatcc ccagaaagac gcccacttcg cggtaaaatt gttcgatggt gactttatgc
     9241 ggcgaactta gcgcggtttg aatcagatca tagaacggcg cataccaggc gttgacggca
     9301 accccgactt ccaccaaaaa ccaggtgacg aagataatca gcgcagtacc gagtattgac
     9361 cagtattgcc aacgatgcgg gctgtagata aaccagaaca atgcaaaaag tcctacgcaa
     9421 acaatgtagt aagcgtaaaa aatcaggaaa tccaacgacc agaaacgcgc ggcgctaatc
     9481 gggatctgcc cggaagcgcc ggtgatacgc gccacccagt cacccccacc ggcttgccag
     9541 aagataacgg caatcaatgc ccaaacaaag gccgagagaa aaaacgttcc cggctttggg
     9601 aaaaaagact taaacatatg cactcctgct aacttcttat cgttttaaca actgctgtgt
     9661 attaaccgca tgaccgctac aaagcgtcag ccaaatgaaa ccgcccattg tgacccatag
     9721 caaggcgctt agttcgccag cctgaccata agaattgttt cgacggtttt cacaaaccaa
     9781 agattgttta cataagcgga ttaacacgcg caggatcaca aatgcaggca ttggtgagca
     9841 ggaaaaaaat cgtgtttacg cgccgttacg tttttaacac tattattgca ctaattcgcc
     9901 ctttgcaatc catcaatgag tagtataaat acgctcagtt accttcattc aatttatgga
     9961 caccaccgtt gaaacgtagt ctgctttttt ctgccgtgct gtgtgcggcg tcattgacct
    10021 ccgtccatgc ggcgcagccg atcactgaac cggagtttgc ctctgatatt gtcgatcgtt
    10081 atgccgatca tattttttac ggcagcggtg ccacggggat ggcgctggtt gttatcgacg
    10141 gtaaccagcg cgtctttcgc agttatggcg aaacgcgacc tggtaataac gttcgcccgc
    10201 agctggattc ggtcgtgcgt attgcttccc tcaccaagct aatgaccagt gagatgctgg
    10261 tgaaattgct cgaccagggg accgtgaagt taaacgatcc gctaagtaaa tatgctccgc
    10321 caggcgcacg cgtgccaacc tacaatggga cgcccattac gctggtcaat ctggcaaccc
    10381 ataccagcgc cctgccccgt gaacagcccg gtggcgcggc acatcgtccc gtgtttgtct
    10441 ggccaacgcg cgagcaacgc tggaaatacc tttctacggc gaagctgaaa gccgcgccag
    10501 gcagtcaggc ggcgtactct aaccttgcgt tcgacctgct ggcagatgca ctggcgaatg
    10561 cgtccggcaa gccttatacc cagttgtttg aagagcagat tacccgtcca ttggggatga
    10621 aagacaccac ctacaccccc tcaccggatc agtgccgccg tttaatggtt gctgagcgcg
    10681 gtgccagtcc gtgcaataac acgctggcgg caattggcag cggcggcgtc tattccacgc
    10741 ctggcgatat gatgcgctgg atgcagcagt atttgtcatc tgatttttat cagcgcagta
    10801 accaggcaga tcgcatgcag acgttgattt atcagcgcgc gcagtttacc aaagtgattg
    10861 gcatggatgt ccccggcaaa gcggatgcgc ttggtttagg ctgggtatat atggccccga
    10921 aagagggtcg tccggggatt attcagaaga caggcggtgg tggcggtttc attacctata
    10981 tggcgatgat cccgcagaag aatattggtg cgtttgtggt ggtcactcgt tcgccgttga
    11041 cgcgctttaa aaatatgagc gatggcatca atgatctggt gaccgagcta agcgggaata
    11101 aacagttggt tatccccgca tcctgattaa tactctgaag gcaaacggtt gatagcgacc
    11161 agtttgcctt tattcatttc gatatagccg cctttacgca aagcagcgag aacttctgcg
    11221 acgacagaac gcgatatgcg tgtacgttga tggatataat tcatcacacc aatacgtgat
    11281 cgcaattctt cattccagtc aatcattgat aataatgttg cgcggatttg ttcgtaggaa
    11341 ttatgcccaa tgagctgcac gtcacgtaat tccagaattc tattttgcca ggctaaccag
    11401 taaaaagcgt ctcgccagag ttgattttgt tcaataagcg taatggtttg tttggctggt
    11461 agatgatatc ccgtacaatt tccttctgat attaatttgt atggtatatc gtttttcatt
    11521 aaaccatcag ccagccccat aatataagga gcctgggtaa taccgataag tacgttttct
    11581 tctctacgca gagagataac tccctctaaa ataacgaaag tatcttcgtt attgacatca
    11641 ctggaaaata taacttgctt ttcattatta aactcgaagc gcgtaccgta tctggacaaa
    11701 catttatcga gcttaccaaa ttcctgaaga ggtttaacta cagataacat ttgcgcgtcc
    11761 tttgcagtaa tgcccgtcaa atccttgacg ggcattattt agattaaatt accagaattt
    11821 cttcggagtg aagaatatta ccaggtatat ttaacaccca cgttcgcgga ccagtcttga
    11881 tctacgtcac caccaccgag gtagttagca tcggtatagg cgctaaagtt cttggtgaag
    11941 ctgaactgag tacccagacc aacacgtacc gcagaccctt cagtaccgtt atcgatggaa
    12001 tcaccgttca catcgttatc gttgttagag tcgtcgtaga cgtaagccag tttgaagtac
    12061 ggagtcagag cctgatcttc gctgtaggtg aaggtataac ctgcatctac acccagttca
    12121 taacgcatgc tgtcgtaaga ctgaccgtca actttcatgt cgttgctcag ctggtagtca
    12181 tcaccagact ggaacagacc agaaacgctg ccgtaaggag tcacgtaacc agcatcaccc
    12241 agtttgaagt cgtaaccggc tttcaagccg aagccccaag cgtcggagtt ggtgctaccg
    12301 tcaacgtaag taccgttgct catggttgca gacaggtcgt tgttgaagtg agagtagctc
    12361 aagctaccat caacaaagac gttgttcgcg aagtgagcag aagagtagat gtaggcagtc
    12421 tggctgtctt gatctacctg accagaacgg tcattcatgt cacctttagc gaagcctgca
    12481 gccgcaccga cgatccactt agcgttgtta ccgtcaactt tggtatcaac accgaccatg
    12541 atgccgttaa catcctgatc atagttgatg gtgccgttgt cgccgttgaa gttaccaccg
    12601 aagtagctta cccatgcgcc gccgttatca gccaggccat gacgagagtt ggtcagacga
    12661 gtaccaacgg tgtcttgttc caggttccag atattggtgt tcgcagacgg gatgctcagc
    12721 gccatgttag cgtagtcggt cagctccatc tgttgcagaa caacggtgtt accgcgctgt
    12781 tcagcctgat aggtgtatgc acccaggtca gctttgttag cagcagagaa ggtcgcgttg
    12841 gtgttagcgt cgtttacccg aataagttct ttatctttgt aatcagcgat agtgccagcg
    12901 ccagttgcgt tgtcgatacg aactttgtag ttaccggttg cagcaacaac gttatcaaca
    12961 gagctgttat ccagttcagt accgttgtct acgtcgccgt taccattgat agtcaggtga
    13021 ccatcagagt tcatagcaac aataccgtaa ccgtagttag acttggaagt atcccacgta
    13081 cggtcgttca ccagatcagc gttcagaacg taatcgctgc tgttgatgtc gaacacacca
    13141 gaaattacgt tgccattatg gtcagcaatg ttgccgctgg tcagttccag tttatcagta
    13201 cggaaagctt cagtatcgaa ctgatcaaca ttcacatcca gcacaccacc gttagtcaca
    13261 gtgatagtat tggtatacag attagaataa ttattccaac cggtagattc ggttaaatta
    13321 acggtactgt agctatcaac agtcaggtgg tctgtagcaa ccacgccatt tgcaccaata
    13381 ttcagtgaag aaccgttggt taaaccaatg gtatctgata ccagagagga atcagtaaca
    13441 tttacctgag agccattatt tacagctaat gtatcaatta ctgaactatt aacggtattc
    13501 cattctgaac tattgttcag tgctacgtta aatacgccgc tctggaatac tttgttgtca
    13561 ataatacggc cccaatcatt agcttcgatg gtggcaccag gggtcaggct gttcggcgca
    13621 acgtcataca tatctgccgg agcaattact tcagcactca tgtttgctga accaacccac
    13681 ttactaccgt tattcagagt aatattcagt tcgtcaacgt tctgagcgtc atctacccaa
    13741 ccgccgttgg tatcaaggac gccgtcgccg ttgctgtcat gaccggtgga atagaagaat
    13801 acgccatcgt tattccagtg acttgtaaat tcgacatcac ccaacagagt tgaatggtca
    13861 aagtttacat tgtttttcat ggaataattt gagccagcat tgtcagtgaa tgacagtgct
    13921 acgtcattct ggccaccatt accagcgtaa tcacttggtt cagcagattt accgaagaag
    13981 ccatcacttt cttccagcgg agtctgtgat cctgaagtta cagtggaatt agacacattg
    14041 atgttgttgt cccactgatg atattcgcta gtataagttg tgttgcttgt attgccttca
    14101 gacagcgcaa tgccagctac acgggagttg ttggtaatat tgatattgct ttcaacatca
    14161 agatttactg caacacccag agcggtatag aacgtttcat tggttgactt agatttatca
    14221 ccatcgagat gtgtgtcagt gaagtaaaga gcttcatagt catcatcaat ggtggaattg
    14281 gatacgttca gagtgaatac gtcaccatcg taccaattgt agtcaagatc agtagtagtt
    14341 ccgttattga aagtatagcc attataacct tcaggcgtaa tgctgtaata accaaatggc
    14401 agttcagaag tgattgaccc atgaattact gaattgctga tatctacctg gttaacagaa
    14461 ccgctagcgt aatcatgatt caagtaataa gtagaaatgg taccgttaac ggtatcgcct
    14521 ttaaagacgg tatcgaaatc gccattggca ttgttatagt aaacaaaacc attgtaagtt
    14581 acgtagttac catctgaacc catcgggtcc atgccgccag catcggcaat tccatcattg
    14641 tcggtgtcat acggccaaac taaatcggtc gtggtgttgt catgataccc ccaggtatca
    14701 tagtttgctg cattagcctg agaggtgata gccagagtgc aagccaatgc taattgtgat
    14761 actacaagtt tctttttcca ggagtgcatt tgtcatccct cctcagggac gtaataagtt
    14821 gatccatcaa ttatcaatgc atagaaaaca tattatgttt cgcgctaacg attatgctgc
    14881 gttacagacg aatggttcaa ttattctttc ccctaagtcc gaatgtggat atatataatt
    14941 tatggagaaa aacacgcatc aaattgataa aagggcgata tttcaacatg
//
LOCUS       DAJOWB010000113        14691 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJOWB010000113 DAJOWB010000000
VERSION     DAJOWB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.113"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            88..444
                     /locus_tag="OHO85_003739"
     CDS             88..444
                     /locus_tag="OHO85_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCQ2673058.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(495..1391)
                     /gene="yhaJ"
                     /locus_tag="OHO85_003740"
     CDS             complement(495..1391)
                     /gene="yhaJ"
                     /locus_tag="OHO85_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCQ2673059.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1496..2197
                     /locus_tag="OHO85_003741"
     CDS             1496..2197
                     /locus_tag="OHO85_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCQ2673060.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2220..2384
                     /locus_tag="OHO85_003742"
     CDS             2220..2384
                     /locus_tag="OHO85_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673061.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2596..3906)
                     /locus_tag="OHO85_003743"
     CDS             complement(2596..3906)
                     /locus_tag="OHO85_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCQ2673062.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3934..5265)
                     /locus_tag="OHO85_003744"
     CDS             complement(3934..5265)
                     /locus_tag="OHO85_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCQ2673063.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5540..6904)
                     /gene="tdcG"
                     /locus_tag="OHO85_003745"
     CDS             complement(5540..6904)
                     /gene="tdcG"
                     /locus_tag="OHO85_003745"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCQ2673064.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6976..7365)
                     /locus_tag="OHO85_003746"
     CDS             complement(6976..7365)
                     /locus_tag="OHO85_003746"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCQ2673065.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7379..9673)
                     /gene="tdcE"
                     /locus_tag="OHO85_003747"
     CDS             complement(7379..9673)
                     /gene="tdcE"
                     /locus_tag="OHO85_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCQ2673066.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9707..10915)
                     /gene="tdcD"
                     /locus_tag="OHO85_003748"
     CDS             complement(9707..10915)
                     /gene="tdcD"
                     /locus_tag="OHO85_003748"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCQ2673067.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10941..12272)
                     /gene="tdcC"
                     /locus_tag="OHO85_003749"
     CDS             complement(10941..12272)
                     /gene="tdcC"
                     /locus_tag="OHO85_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCQ2673068.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12294..13283)
                     /gene="tdcB"
                     /locus_tag="OHO85_003750"
     CDS             complement(12294..13283)
                     /gene="tdcB"
                     /locus_tag="OHO85_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCQ2673069.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13382..14320)
                     /gene="tdcA"
                     /locus_tag="OHO85_003751"
     CDS             complement(13382..14320)
                     /gene="tdcA"
                     /locus_tag="OHO85_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCQ2673070.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3849 a         3910 c         3410 g         3522 t
ORIGIN      
        1 tataacatcc gaaaaagaaa gatacgaata gcatttacac aatacccgga atagactttc
       61 gtcactttta attaaaggga tgtttttatg cagtggtatt tagccgtatt aaaaaattat
      121 gttggtttct ctggtcgcgc acgtcgtaaa gagtactgga tgtttactct gattaacgcc
      181 atcgtcggcg ctattatcaa tgtcattcaa ttgattttag gtttagagtt tccattcctt
      241 tctctcattt acctggcagc aacaatcatt ccggtcattg ctctgtgtgt tcgtcgtctg
      301 cacgatactg accgttcagg tgcgtgggcg cttctctatc tggtaccgat cattggctgg
      361 ttagttctgt ttgtatttgc ctgtttagaa ggcaattctg gcagcaatcg ttacggaaac
      421 gatcctaagt ttggttcaaa ttaatcttag aattggggcg acatttcgcc cctttttatt
      481 aataataaca aatattattt tccgctaaaa agtttgggaa tttcccgcag acaccaggat
      541 ttcgcttccc ccatactgtc acgacgccag gccataataa tatcgatctc gctggtcgat
      601 tccgggctga cgacacgcaa ccgcccttcc gcaatatctt tttcgaccat cggatacggc
      661 atcgtcgcca cgccaagccc cgccagtaat gcctgacgtt tatcttcaat cgtgctcacc
      721 gttaagcgcg gctgtttgtc cagcagctgt acggtcaaca ccgggcgctc acgagcggta
      781 tccgctaccg caattccacg atatttcacg cgcgtcactt cagataacgg ttccggctcc
      841 tgatgaatcg ggtgatcagg cgcggcaacg tagacgttca ttaaggtata gagcttgcgc
      901 gagttgatct ccgacgagga acgaaaatgc atatccggcg cgataacgat atccgcccgc
      961 ccctgctcca gtcgttccca cgcccccgcc agcacttcgg tgatgattgc cagttgggta
     1021 ttggcttttg ccgccagttt gtcgattaac gggaaaaagg caggtgtcgg taccagcgcc
     1081 tcggtcacaa tggtgagatg cgtttcccaa ccgcgcgcga gggcttccgc atcggtagtc
     1141 agtttatctg cggcttccag caaaacgcgc cctcgctcca gcaacatcct cccgacattg
     1201 gtgaatttgg tgcgatggcc cgagcggtca aacagcacca catccagctc ttcttccagt
     1261 ttttgcatgg tgtagctaag tgcggaaggc acgcgtccca gctcatccgc cgccgccgca
     1321 aaactgcccc ggcgatcgat cgcatccata acccgtagtg cttccagcgt taatgccctt
     1381 tctttggcca tttcgttctc attcaggaaa tttgaacata ccgggcagaa tatctggcta
     1441 acaatgtagc gtccagcccc ttaccataaa aggaagtaaa gagaggtcaa gaattatgat
     1501 tactacccga actgccagac agtgtggaca agcagactac ggatggttgc aggcccggta
     1561 tactttttcc tttggacact acttcgaccc gaaattgtta ggctatgcct ccctgcgtgt
     1621 gcttaaccag gaagtgctgg ccccaggtgc cgcctttcag ccgcgaacct atcccaaagt
     1681 cgatatttta aatgtgattc tggatggaga agcagagtat cgcgacagcg aaggcaatca
     1741 tgttcaggcc agcgccggtg aggcgttgct gctctctacc cagccgggtg tcagctacag
     1801 cgaacacaat ctcagcaaag acaaaccgtt aacacgaatg cagctttggc tggacgcctg
     1861 cccgcagcga gagaatccgc tgattcaaaa actggcgctt aatatgggca agcagcaatt
     1921 aatcgcctcg ccagagggta cgatgggaag cctgcaatta cgccagcaag tgtggctgca
     1981 ccatatcgtg ctcgacaaag gcgaaagtgc gaatttccag ttgcatgggc cacgcgcgta
     2041 tttgcaatcg attcacggga aatttcatgc gcttacgcat catgaagaga aagcggcgct
     2101 gacctgcggt gatggggcgt ttattcgtga cgaggctaac attacgctgg ttgccgattc
     2161 cccactgcgc gctttgctga tagatttgcc tgtctagttg tttttacagg gagatgatga
     2221 tgagtaaaaa atcggccaaa aagcgccagc cagtgaagcc cgtggtggcg aaagaacctg
     2281 ctcgcaccgc caaaaatttt ggctatgaag agatgttgag cgagctggaa gccatcgtcg
     2341 cggatgctga aacgcgttta gccgaggatg aagctaccgc gtaaagacag gttgccggat
     2401 gcggcgaaaa cgccttatcc ggcctacaaa atccaacaac attaacgaat taaacaaccc
     2461 gtaggccgga taagatgcgc cagcatcgca tccggcatca ggtacaaaac acaacaacat
     2521 caacaagttg agcaccccgt aggccggata aggtgcgcca gcatcgcatc cggcacaata
     2581 ctcaaaacct ggcgtttatc tggccttgct cgccataatc tcgataatct gccgatccgt
     2641 ttgctgcatc gaatggcttg ctaacgcaca caggttggca atcgactgct caacatcatg
     2701 cgccacgatc ccttcattgc cggtcacggc ggtatcatcc agcgccatta acaccgcttt
     2761 ccacgcagcc gaagcactgg tcgaaacctt catcgcgcag ctgttcgacg caccatcgca
     2821 aatcatgccg ctgacatcgc cgatcatact gctgatcgcc atcgagatgg tttcataacg
     2881 cccatccacc agccatgcca tcccggcggc ggcccccatt gctgcggtcg ttgcggcaca
     2941 cagcgcagac aaacgcggta actggttatg gatgtaaatt gcgctcaaat gagaaagcat
     3001 cagcgcacgc gccagccgtt catcatccgc tccgaagtgt tctgctacca ccaccacagg
     3061 cattgttgcg gtgatcccct ggttacccga gccggagtta ctcatagccg gaagcgtagc
     3121 gccgcccata cgcgcatcgg atgccgcgct ggtacgaatc acaatggatg aagagagatc
     3181 tttcgccagc aaaccgcgct cgcactgttt ttccagcgtc gcgccaatat gcagccccca
     3241 cttaccgctc aaaccttcct gcgataacgc acaatttagc ttcgcggaat cgagaataaa
     3301 gcggatcgcc gcaaacggga cttcattgac gaacttcagg atctcagcca gcgtcgttct
     3361 ggaaagcacc gtcagcggag actcttgctc gccctctgcc acacacgcct gctgggtaaa
     3421 caccacacca ttgtgcgtct caatatgcac aatgttggta tgcccgccga cgatggtgac
     3481 acacgcccac ttctcaccgt tccagacttt ggcgcgtgag aagaggattt cattgcaagg
     3541 ttcctggatc ttaacggaga ctttccccgc cgccagcagt gctttggcat cggcaattgc
     3601 ctgcgcagtt gcgtctttca gcacttccag cccggcgttg gcatttccac ctaacgcccc
     3661 cagcgccgcc gcaatcggca gccccaccat tcccgtgccg ggaacggtga cgcccagacc
     3721 gttcttcatc agatttggcg aaacccaggc ttctacacgt tcaaccggac cttccagttc
     3781 tgccgcagca accgccgccg ccagcgccag tgaaatcggt tcagtacatc ccagcgccgg
     3841 ttttacttcc tcctgaacgg cgaggatgta acgctgccat aacggattta aagtcgaatc
     3901 aaacatatta aaaaccttaa aatttcaggt aaatcaagaa aatgccagga acggagaaac
     3961 acaaagcaac aaaccagtga cgataatcag gtacagagac atccctttgt atttgtgcaa
     4021 tgccggtact ttgtaaacca gccacgccgg gatcaggcac cctaccatgc cgaaaatcgg
     4081 gctacagata gaggtgaagc tcaacaccgg tgcgttcagt acgatggcgc tccaggccag
     4141 caaaatggcg aaaatcatga tgccgcgctg aacaagattt tcgttaatct tctcggcagg
     4201 catcttgcga cgcaggatgt tcattacgat cccttgcgtt gcttcgcgaa agcctaaata
     4261 gacgccaaag aatgcagtca tgacagcaaa gatattgaga atgacgctga ccactttcac
     4321 ccacgctgcg ccgtcaccgc taataaactg tgcggcaatc gccagcgcgg aaatattctg
     4381 ctcataggct tttaccgctt cgtcatgtcc catcgccagc gtgaacgaca cggcgtagaa
     4441 aaagacggtg acaaacaaaa tgccaaacgc gatattcatt gcccgcaatg ctttatgccg
     4501 cgccacttca attgattttt cccgcgagcg ataagagatc accatcgggc ttaacgtctg
     4561 gataaacaga atcgacgtca gggtaaacgg cagcgtaata atggcgtttt tcaccagcag
     4621 ccccagcggc ggtagcgaac cgacgttata cagatgccac attcccacca tcgacacgcc
     4681 cagcgccgcg accaccagca gcttggtcag caccatgccg gtcgaaattt tgaataacaa
     4741 tttctcgccg cgtgaagaga tcgccaccag aatgcaaatc agtaccagac cataaaaggg
     4801 actgtctgac agcaaccctt ccgtcacgcc gaaggtatgc agataggaag cactatcgtt
     4861 ggtgatggcg gtggaataga cgaacatcca aatcaccagc attacgaaat agagcgcacc
     4921 taacaggatg ccccagtttt tacctaaata accgctaatg acactcgggt aatctttaca
     4981 ttctggtgat tctgccagcg tattaataaa caaccgctga aacagataca ttgccgggta
     5041 accaatcacc gatgagagca aaaataccca caatcccatc aaaccgacct gcaccgggag
     5101 aaaaacaatc cccgcgccaa tcgccatccc gatactcata atcacccagc cggtgtcagt
     5161 actgtcgaat ttgatcgctt ctcgccactc gctctcactc attccagcac gacccgccgg
     5221 ggtcgaagcg tctgcaatga cgcctttatt cgatgcaatt tccataattt ctcgctcaat
     5281 attttgtagg gcttattatt tttttccgag ccgcatcaag gcgatatgcg gtctacgttt
     5341 ttgcaggcga gttattagaa taaagaaatg atacgcgcga ggtcagagaa aatcttcaca
     5401 atcaagactt tgaaataacg gagatggata agaattttct actaaattaa tcgcaagaga
     5461 atattcattc tctatttgcg gcagatcaca aaaaaaaggt gcacatttgt gcacccaagg
     5521 atgaaagctg acagcaatgt cagccgcaga ccactttaat ggccagtcct ccgcgtgatg
     5581 tttcgcggta tttatcgttc atatctttgc cggtttcata catcgtctcg atcactttat
     5641 cgagtgaaac acgcggtgcc gaggtgcggc gcatcgccat ccgcgcggcg tttactgctt
     5701 tcacggcatt aatggcatta cgttcaatgc acgggatttg tacctgtccg gcaaccggat
     5761 cgcaggtcag cccaaggtta tgctccatcg cgatttccgc cgcattgcat acctgcgccg
     5821 gactgccgcc cagtagttca gttaaccctg ccgccgccat tgaacaggcc acgccaatct
     5881 ccccctgaca gccgacctcc gcgccagaga tggaggcgtt cattttatac agcgcgccaa
     5941 tagcccccgc tgccagaaaa tagcgggcaa ttgaccgctc gtttaccgga cgacggaact
     6001 tatcgtaata agccagcact gccggaataa tgccgcacgc accgttagtc ggtgccgtta
     6061 ccacgcgccc gccagctgcg ttttcttcac taaccgccag cgcgtacatg ttgatccagt
     6121 cgatgacatt catcggatcg ttagagatgt tatcgctaga aaccagctga cgacgcagcg
     6181 ctacggcacg acgcggcaca ttgagcggac caggcagcac gccttcagtg ttcatcccgc
     6241 gttcaatacc gtcatgcatc acttgccaga tacgggcaaa ccctgcgtca atttccgctt
     6301 tactgcgcag cgctagctcg ttgtgcatca tcaggccaga tatagacagg ccgttgtaat
     6361 cacacatttt cagcagttca cctgctgagt ggaaatcgta aggtacggac gtttcgacat
     6421 cgtgcgacag gccgaagtgt tcttcttcga caataaaccc gccgccgaca gagtaatagg
     6481 ttttacttaa tagcgcttcc tgtcccttcc aggcagtgat ccgcattccg ttctcatggc
     6541 gaggcaacat ttcgggatgg aagataatgt tctttgctac aggaaaatca acaatatgcg
     6601 caccagatgc cactggcagc cgcccgctgc gcgttactaa ctctataaat gcagggatct
     6661 catcaatgac aacatcctgc ggactgtttc ctgccagtcc catgataatg gcgacatccg
     6721 tggcatggcc tttgcccgtc agtgacaacg acccatacag atcgaccaca atatggctcg
     6781 tcgcggttaa taagccgcta ctttccagcc gatcaataaa actttttccg gcattcattg
     6841 gccccacggt atgcgaactg gagggaccaa tcccaatttt gaaaatatcg aatgcactaa
     6901 tcatatccac accctcggat tgccgttcag tgaagtggag cggaacgacc ttgcgaccgt
     6961 cccgctcacg aggctttacg cactacgtac tgcgatagct tcaatttcca gcttcacatc
     7021 tttcggcaaa cgcgcaacct gcacacagct ccgggtcggg taggtcgcct gatgctcatc
     7081 gaagaactgc ttatagactt cattgatggt ggcaaaatca ttcagatcgg tgatgaacac
     7141 ggtcatcttg atgatatcgc ccacgctcag cccggcagca accacgatcg ctttgacgtt
     7201 ttcgaggctt aaacgcgcct gatcttgcac atcagccggg atctcaccgg tctgcgggca
     7261 aaccggaatt tgcccggagg tgaagaccat gctgcctaaa tcaacgccct gaacataagg
     7321 gccgattgcg cctggggcac gttgcgtttc gataatcttt ttcatacatc ctccggcgtc
     7381 agagcgcctg ggtaaaagta cgtgaaataa catcctgttg ctgttcacgg gtcagtgcgt
     7441 tgaagcgcac ggcgtagcca gagacacgga ttgtcaggtt aggatatttt tccgggtgct
     7501 cgatggcatc cagcagcatt tcccgattca ttacgttgac gttgaggtgt tgaccgcctt
     7561 cgacatccgc ttcgtggtgg aaatacccat ccagcaggcc gacaaggttg gttttacgta
     7621 ctggatcttc tttgcccagc gccgcaggaa cgattgagaa ggtgtacgag atcccatctt
     7681 tggcgtaggt gaacggcagt ttcgccaccg acgtcaatga ggccacggca cctttgcggt
     7741 cacgaccatg catcgggtta gcgcccggcg cgaacggtgt tccggcgcga cgaccgtccg
     7801 gcgtattacc ggttttctgg ccgtacacca cgttagaagt gatagtcaga atcgactggg
     7861 tagggacggc gttgcgatag gttggcagcg ctttaatttt cttcataaag cgttcaacca
     7921 ggtcgcaggc aatgctgtct acgcgctcgt cgttgttgcc gtactgcgga tattcaccgt
     7981 cgatttcaaa gtccaccgcc aggccgtttt cgtcacggat tggtttcacg cgggcatatt
     8041 tgatggcaga cagggagtcc gtcgccaccg acaggcccgc gatgccgcat gccatagtgc
     8101 gatagacatc acgatcgtgc agcgccatca gcgaagcttc gtagctgtac ttgtcgtgca
     8161 tgtagtgaat gatattcagc gcgctgatgt actgcaccgc cagccagtcc atgaagtgat
     8221 cgaggctgtc catcactttg tcgtaatcca gcacgtcgtc catcagcggt gctgttttcg
     8281 gcccgacctg aatcttcagc ttctcgtcca ccccgccgtt aattgcgtag agcagcgttt
     8341 tcgccaggtt agcgcgtgca ccaaagaact gcatttgctt accaatcacc attgggctga
     8401 cgcagcaggc aatcgcgtaa tcgtcgctgt tgaagtcagt acgcatcaga tcgtcatttt
     8461 catactgcaa ggaagaggtg acgatcgaca cctgcgcggc atattttttg aaggcaatcg
     8521 gtaattcttc cgaccaaaga atggtcaggt taggttccgg tgccggcccc atagtgtgca
     8581 gggtgtgcaa atagcggaag gagtttttgg tcaccagcgt acgaccgtcc agccccatcc
     8641 cgccgatcac ttccgtcgcc cagattgggt cgccggagaa cagcgaatca aattccggtg
     8701 tacgcaggaa gcgtaccata cggatcttca tgatgaagtg atcgatcagt tcctgtgcct
     8761 gctgctcatt gagtacgcca gctttaaagt cgcgctcaat gtagatatcg aggaacgatg
     8821 ccgtgcggcc cagcgacatc gcgccgccat tttgcgattt cactgccgcc agataagcga
     8881 agtagagcca ctgcaccgct tcctgcgcat tctgcgccgg gcgagagata tcaaagccat
     8941 atttcgccgc catttcctga atctgcaaca gcgcatgacg atgctctgcc agctcctcac
     9001 gcagacggat ggtggcttcc agatcctcgc ctttttccag acgagactgg agatcggcaa
     9061 attgcagttc gcgttcacgt accagataac tgatgccata cagcgctacg cggcgatagt
     9121 caccgataat gcgcccacgg ccatagccat ctggtaaacc ggtcagcacg ccagatttac
     9181 ggcagcgcag catatccggt gagtaaacat caaatacgcc ctggttatgg gttttacgca
     9241 gatcggtaaa cagatattca aattcactgt ccatttctcg gccataggcg tggaatgaac
     9301 ttttaatcat attaatgcca ccgaacgggt gtagcgcacg tttcaacggc gcatccgttt
     9361 gcaggccaac aattttttcc agcggctggt taatatatcc cgcatcatga gcggtaattg
     9421 tggtggcaat attggtatcg aaatcaaccg gcgcgtgggt tgcattttcg atacggatgc
     9481 cttccattac tttttcccac aattccgtgg tggcaggcgt cgcttcggcg aggaaagatt
     9541 catcgccttc atacggtgta tagttatgtt gaataaaatc gcggacatta atttcgtttt
     9601 tccagtccgt acctttaaag ccaagccatg cgtcggcgta cagcttatcg ctggtatcaa
     9661 tatctacctt catgaaaaat aatctctcta caatacttca actaaattat gcaaattctg
     9721 cgggcgcgtt aactttgcct aaatgaatgg catccaaagc aatcattttt tcttcgttag
     9781 tcggaataac ggcacaaatg acacgcgcat tttcactgga aacaattcgc tctccaaaag
     9841 agttagatcg attattcatt tctgtatcaa tcactacgcc taatacagcc aaatgttcca
     9901 tgaccagacg acgaattaaa cttgaattct ctcctattcc gccggtgaat ataattccat
     9961 ccaggcgacg taatgaagct gcgtgtccgg caatatgacg ggcaattcgg tgaacaaagg
    10021 ttttaattgc cagttgcgcg cgttcgtgac cttcatgcca ggctttttcc agaacacgta
    10081 aatccgaaga aagaccggaa atacctaata atcccgactc tttattcact acgcgttcca
    10141 ggtcacccag actctggttg gtttggctgg cgacccagga catcgcacca aagtcgacat
    10201 cgccactgcg ggtacccatc atcaagcctt ccagcggcgt cattcccatt gaagtatcaa
    10261 cactctgacc gttgcgaacc gcgcagattg acgcgccatt gccaagatgc gccacaacca
    10321 ggccggagtc atcttccgcc agattcagca gcgaatgtgc gcgctgggaa acatagcggt
    10381 gcgacgtgcc gtggaaacca taacggcgta cacctaactc ttcataatat ttccacggca
    10441 ggccgtataa ataagcttcc ggagccatcg tctggtggaa actggtatca aataccgcca
    10501 cctgagttac gcccggaaat aattgctgcg ccgattcaat accacttaaa ttggcgtaat
    10561 tatgcagggg tgccagtgga gaaacgcgac ggatattatc aatgacttca tcggtaataa
    10621 tggcggactc ggtaaaaata ctgccgccgt gagcgatgcg gtggccaatt aaggccacac
    10681 tgtcatttaa attccgtttt tccagttcaa atgcaattgc cttcaatgca ccttcgtagc
    10741 tgtggtgagc cagcggtgct ggctctcccc catttacgga taagaatgca ttttccgagt
    10801 taataccgtc ggcaatacct gacattaata cttcacagtc gctggcatcg agcacggaaa
    10861 acttaatcga agacgaacca cagttaataa ccaaaacaac cggaaattca ttcatctctt
    10921 ttctcatcct gagttacgga ttaaaacagt ttgtatacga tgttcaggat ggtcagcaga
    10981 ccaatcacgg taacaaacac gttatccaga cgaccacggt atttcgccag agacggcgct
    11041 ttacggatgg catacatcgg caacaggcac agcagggatg cgataatcgg tgcgcccatg
    11101 gcttcaatca ggtcaaggat gttcgggttg gcgtaggcaa caacccaggt ggagcccatg
    11161 atgaagatca tgctgatagt gttcagttta cccagcgaca ctttagtttt gtcgccttta
    11221 taaccaaact tcaggaccag gccattcaga ccttccagcg ttcccagata gtgaccgaag
    11281 aaagatttga agatagccac gagtgcgatg atggaagccg catattccag tgtaatcgcg
    11341 aacgttgttt tggtaccggt catggacgca aagtggttag ccagataaga aagcactgga
    11401 atattctgcg ctttggcttc cgccatgttg gccggagaca gagtaaacag gcagctaaag
    11461 gcaaagaaca tcaccactgc aaccatcagc atgctggcac gagaaatgat ttgggaacat
    11521 ttacgttcgg tgaagtcgcg accgaagtct ttctcatact cttcacgctt agaaaccacg
    11581 aaggaagaga cgattggcga gaagttaaag gagaaaacca tgatggaaat ccccagccag
    11641 acagtgatca ggataccgtc atgaccggtt aacgacagcg aaccgaggtc aacctggtcg
    11701 ataactgcag agttccagta agggatcagc gacaaagaaa tcagcaccag gctggcgata
    11761 aacggccata ccaggtagct catcacttta accatcagat ccttaccaaa ccagatgacg
    11821 aaagccatca gcagcaacag gaacagcgcc acaaagccgc gattcagcgg tgcaaagccg
    11881 agctggtttt cccagaacgt cataaaggta ttggtaatag taacgccata aatccacagc
    11941 agtgggcaaa tcgcgaagaa gtacaggaac gtgataacca cgccgccagt tttaccaaaa
    12001 tgctcttcca ccgtttccgt aatgttgccg gaagggttag agccagaaag acacagacgc
    12061 gccagcgccc ggtggcaata aaacgcgatg gggtatgcca ataccaacat cagaagaatc
    12121 gggatcagtc cgccaaaacc tgcgcggata gggaagaaca gcaccccggc gccgattgcc
    12181 gtaccaaaca agcctaacgt ccatgtggta tctgatttac gccaggacga ttgttttgtc
    12241 tggctggata caatgctatc tgaagtactc atatcctatc ctcaacgaat taattaagcg
    12301 tcaacgaaac cggtgatttg agagacgcga gaaagatcga tattgccgcc ggaaataata
    12361 ctgacggttt ttctgttttg aatatattgg tctaatttac cgcttaataa tgcagcacat
    12421 gccagagcgc ctgcgccttc ggtgacgact ttattgcgct gaattaaggc aatcatactg
    12481 tttctgattt cgtcttcgct gaccagcacg atgtcatcga ctaattcacg aacgatttcg
    12541 taagttaaat tacccgggcg ggagacatca caaccatccg ccagggtgcc ggtagttcgg
    12601 tgcgtggtta tttctccgga gtggaaagaa gccgccatgc cgtgaacgtt ttcagactgt
    12661 acgccaataa cacgaatggt cgggttaata gatttaattg ccaccgcaat accagcaatt
    12721 aaaccgccac caccaattgg cacaatcacg ttatcgacat catagagatc ttccataatt
    12781 tccagaccaa tcgttccctg gccagcaatc actttcggat catcgtaagg tgggataaaa
    12841 atacggcctt ccatttcgac aatttcgctc actttagcga tagtgtcgtt gaagttatca
    12901 ccatgcagaa cgacttctgc ggagtagtcg cacgttgccg ctactttgga ttttggcgca
    12961 ccttttggca tcaccacttt accgtcgata cccagcatcg cgcaggacag ggaaacccct
    13021 tgcgcatggt tgcccgcaga acaggccacc acgcctttgc gtttttccgc atcggtcagt
    13081 gaacttaatt tattaaatgc gccacgaatt ttaaatgaac ccgtacgctg catattttca
    13141 aacttcagga atatttcacc tttgcaacgt tcactaaaat agttggagcg aggcatgcct
    13201 gttttataaa ttcgcccagc cagtcgttgt ttcgcttcaa taatgtcatc aatagcaacc
    13261 ggcagatcgt atgtaatatg cattataaaa cctcttcgcc tgaattaaat atgtaggtaa
    13321 ccgtaaccga cacctgcaag acagacaggt ggattattta tgtttaaata aaaacaaata
    13381 actaaccaac ttctattaat tgccttcgtc tacacccatt ataagatgaa tactctttgg
    13441 ctaattccac caaaaccgat gctgcttttt taatacgata atttttcgac cataccgcgg
    13501 catattgtgc cacaggtaat gtttcttcaa ccggaatagt aataaattga ttagaaccaa
    13561 aaggtgacgt catatcacaa ggaattacag ttaagaaatc agcattgaga acaagattat
    13621 aaattgtcac gactgagtcg gttttaacga tgttttcaat actgatgcca tttctttgta
    13681 acgtagtaag cagttcgctg tagtacccca tattagtttg tggcaacacc cactgttcgt
    13741 tcttcaacga ctccagcgtg gtggtgccgg tgcatgttcg ggacttactg gctaccagca
    13801 caaactcgga ctcgaacagc ggctcaacat gtaaatcctg aagcttcatt tctgcactta
    13861 acgtaccaat cgcaaaatcc agtcgaccgt cgcggattgc cggtaagaac gaagacagtt
    13921 gcgcttcata catagaaacc tgcgctttcg ggaacacctc tttgaacttg ttgatcatcc
    13981 ctgacataaa agtaaaacca atcaatgaag gaaaaccaaa tgagacttcc accaccgcct
    14041 cagaagacat accgcttatc tcattaacca tatttttcat ttcacgggta atggattcgg
    14101 aacgggagag taacaattga ccggcaggtg ttaatgttac accggtattt ttccgcacca
    14161 ctaattccac accaaaataa tcttcaatat cgttaatgat tttactgacg gccggttgag
    14221 ttaaccctaa ttcttttgca gccgagccga tagaaccact tctaatgact tcctgaaaga
    14281 ctaccaggtg ctgcgttttc ggaagaagaa tagtgctcat aacgacctac gttaattacc
    14341 tcattgacgg catgaagtgt atcaaaatga aatgaacagg atatgtgcga ccactcacaa
    14401 attaactttc aatactttcc agagtatcgt tattacaatt aaataccttt aaatatcaac
    14461 aagttaaagt ataaaaatca gcataaaccc tgatttttgt caaaaaaaat gacggggata
    14521 accatatttt atggcgataa catcattcgt tatgtggtta atacaaaaaa ggggctgaga
    14581 ccatatttca agcaattacc ggataattac caggcaaaat ttgagttaaa tttaatgagc
    14641 aagttctcaa attttattat aaataaacca ttttcagtga taaataatgc g
//
LOCUS       DAJOWB010000114        14569 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJOWB010000114 DAJOWB010000000
VERSION     DAJOWB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.114"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            86..916
                     /locus_tag="OHO85_003752"
     CDS             86..916
                     /locus_tag="OHO85_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCQ2673071.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDNELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1057..1830)
                     /gene="uxuR"
                     /locus_tag="OHO85_003753"
     CDS             complement(1057..1830)
                     /gene="uxuR"
                     /locus_tag="OHO85_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCQ2673072.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2045..3505)
                     /locus_tag="OHO85_003754"
     CDS             complement(2045..3505)
                     /locus_tag="OHO85_003754"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCQ2673073.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETIQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTVTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3586..4770)
                     /gene="uxuA"
                     /locus_tag="OHO85_003755"
     CDS             complement(3586..4770)
                     /gene="uxuA"
                     /locus_tag="OHO85_003755"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCQ2673074.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIIEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5110..6453
                     /gene="gntP"
                     /locus_tag="OHO85_003756"
     CDS             5110..6453
                     /gene="gntP"
                     /locus_tag="OHO85_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCQ2673075.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6696..7598)
                     /gene="fimH"
                     /locus_tag="OHO85_003757"
     CDS             complement(6696..7598)
                     /gene="fimH"
                     /locus_tag="OHO85_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCQ2673076.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7618..8121)
                     /gene="fimG"
                     /locus_tag="OHO85_003758"
     CDS             complement(7618..8121)
                     /gene="fimG"
                     /locus_tag="OHO85_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCQ2673077.1"
                     /translation="MKWCKRGYVLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8134..8664)
                     /gene="fimF"
                     /locus_tag="OHO85_003759"
     CDS             complement(8134..8664)
                     /gene="fimF"
                     /locus_tag="OHO85_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCQ2673078.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8674..11310)
                     /gene="fimD"
                     /locus_tag="OHO85_003760"
     CDS             complement(8674..11310)
                     /gene="fimD"
                     /locus_tag="OHO85_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCQ2673079.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAVFFIRLVVACAFAAQAPLSS
                     ADLYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYKSSDRSSGSKNKWQHINTWLERDIIPLRFRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11377..12102)
                     /gene="fimC"
                     /locus_tag="OHO85_003761"
     CDS             complement(11377..12102)
                     /gene="fimC"
                     /locus_tag="OHO85_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCQ2673080.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12139..12678)
                     /locus_tag="OHO85_003762"
     CDS             complement(12139..12678)
                     /locus_tag="OHO85_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2673081.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12743..13291)
                     /gene="fimA"
                     /locus_tag="OHO85_003763"
     CDS             complement(12743..13291)
                     /gene="fimA"
                     /locus_tag="OHO85_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCQ2673082.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
     gene            complement(13772..14368)
                     /gene="fimE"
                     /locus_tag="OHO85_003764"
     CDS             complement(13772..14368)
                     /gene="fimE"
                     /locus_tag="OHO85_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCQ2673083.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT         3621 a         3713 c         3449 g         3786 t
ORIGIN      
        1 gtcatttgtt tacttctctt tttactacag aaaaatttat ctatttttct aaacatcagt
       61 gaaatactcc taaaaggtaa ctattatgtc tatgccatta agcaatgccc ttcagagtca
      121 aattattaca gataatcact tccttcatca cccaaaagtc gacaatgagt taacacgtaa
      181 atatgaacga gctcgcctgg atacggaaaa tatctatctt ctgcctcttg ctcgtggcaa
      241 taaccataat tacgatggaa aaagcgttgt cgaaattaga aaacttgata ttagtaaaga
      301 gtcctggcca tttaattatg tgacagaagc atgcagagaa tccgatggta ttacaacaac
      361 aggacgtatg ctctatcgaa acttgaaaat cacctcagct ttagacgaaa tatatggtgg
      421 tatatgtaaa aaggcgcatg ctgctacaga attagcagaa ggtttacggc taaatttatt
      481 tatgaagagc cctttcgatc ctgttgagga ctatacagtt catgaaataa cattaggccc
      541 tggttgcaat gttccaggtt acgccggaac cacgatagga tatatttcaa cccttccagt
      601 ttctcaggca aaaagatgga ccaatgagca gccaagaatt gatatctata ttgatcagat
      661 aataactgtc tcaggggtag caaattcttc cggattcgcc cttgccgcat tacttaatgc
      721 taatattgga atgggaaatg acccaataat tggtatagaa gcctatcccg gtaccgcgga
      781 gatccatgca aaaatgggat ataacgttat ccccggtgat gaagatgcgc cactcaaacg
      841 catgactctg caaccgtcat ctcttccaga actgtttgaa ttaaaaaatg gtgaatggaa
      901 ttatattggt aaatagccac tgagaagaat gagtactaat attcaggtta tacagatatt
      961 taaacaatac cctgcaattt acaaagtaat actcattgcg acttcttaat ttaagaaagc
     1021 gcaaggaacg tttacccttg cgcttattat aataagtcag gcgtcgactt tatccagcgg
     1081 ccatgaatca aacagatagc catcaaaata aatatcgtca acgttcgaga attccagcag
     1141 acgttgctta acgttttcca gatgctgcca cattgccagc ttcgccgctc gggcatcttt
     1201 tttgattaac gcggcgagga tctgtttgtg atcgcccaac cactctttgc gatacaggct
     1261 gtcatccaga tggctgtgca actggatcca cattggattg ttttcccgcc actgccagga
     1321 ctgacggaac agctccacca gcatgctgtt atgcgttgct tcggcaatag cgagatggaa
     1381 ctgcatgtca ccgctttcgc tgctgcccgg cgcactggaa gccagttcac gctcttccag
     1441 ttgcaatgcc tgacgcattt tgacgatatc ttcgcgggta gcctgcaaag cggcaaactc
     1501 ggcgatgttg ctctccaata actgccgcgc ctgtaacagc tcaaaaggac cggcatcgtt
     1561 gcagacgttg gcatccggac tgtctgtgtt ctggctgcct gagttatcaa gaacatagat
     1621 accggcaccc cggcgtactt ccaccagccc tttgatctcc agcatgatca gcgcttcacg
     1681 taccaccgtc cgcgtgacat caagcatttc tgcaatttca cgctccggcg gcagccgttc
     1741 gccaggattg tacggcgtct ttatgatcag atcgcggatc atcgccccga cttcctggta
     1801 aggtctttgc gcagaggtgg cagatttcat ggtgttgtgt ccgttaggtg cggttaatca
     1861 aatttttgcc ggatgcggcg tgaacgcctt atccggccta caacgggcaa ttttacggcc
     1921 cccgttaatc tcaaactata ccattagcac tggaagttgg cacgtacagg tgggcaaaaa
     1981 aacgaaaact ggtcaaccaa atatcagttt gattgaccag taagtctgta gggtaatcag
     2041 ttagttacag cgcagccaca cactcgcgcg caccgcgttc gcacagctgc tgatatgccg
     2101 ccgtcactgc gccaacaaag tcggcattct gcggcagatc atcggcaaaa ataccgctca
     2161 ggcccagcag cgctttcacg cggtctgcgc cctgatactg cgcgttgatc ttctggaact
     2221 ccgccagcat cgggtcgacc acgtcaatgg cattaccctg ctcatccacg ccctgggtgt
     2281 aacgcatcca gccagccacg cccagcgcca ggtgacgcca gctgccgccg ttttgcaggt
     2341 gcagacgcac cgggtccagc agacgctgcg gtaacttctg gctgccgtcc atcgcaatct
     2401 gccaggtacg gtgacgcaga gacgggttgc tgaaacgctc gatcagcagc gtcgcatagg
     2461 cgttcaggtc tgtaccttcc ggcatcgaca gcgttggcgc ttgttcctgc atcatcaggg
     2521 caaaggccgc tttgcgataa gccgggttag tcacggtgtc ggcaatggtt tcatagccgc
     2581 cgaggtaacc gaggtacgcc agaaaagagt ggctgccgtt cagcatacgc agcttcatca
     2641 tttcgaacgg cacaacgtct gcaacgaact gtgcgcccac tttatcccaa tccgggcgac
     2701 cattaacgaa gttatcttca atcacccact gacggaacgg ttcgcaggca atggcgcacg
     2761 ggtcgtaaac acccagctgg tcagcaattt cctgtatggt ttctggcgtc gccgccggaa
     2821 cgatgcggtc aaccatggtg cacgggaagg tgacattttc ttcaatccat gccgccagct
     2881 gcgggtcacg cgcctgagcc agccccagta ccgcgacctt cgccacatga ccgttttcac
     2941 gcacgttatc gcaggacatc accgtaaacg ctttcaaccc tttttcacga cgcagacgca
     3001 acgcttcgac gatgtaacca atcgcggact tcggcgcagt cgggttttcc agatcgtgct
     3061 tgatcagcgg gttattgaga tccagttgac cgcttgccgc atcagcgcag tagccttttt
     3121 ccgtgaccgt tagagagaca atcgccgttt gcggacgcgc catcgcgttg agaatacctt
     3181 cgcagccatc gatttccgga tgcagcgctt ctttcatcga accgataatt ttcagctcgg
     3241 tgctctctgc gcctttttcc gctacggtgt acagcagttg ctgttttttc aggttttcga
     3301 tcagcacgcg gtcgttgcct ggcatcaggt taacttcgca gatgccccag tcgctgtcgg
     3361 tgctttccag cagatgatgg gtatacagcg cctggtgcgc gcggtgaaac gccccgcaac
     3421 cgagatgcac aatgcgtgat tccagacgag aatgatccca tgacgggcgg gcaaccggca
     3481 gattgctgtc aacaatagta gtcatcgtaa actccagaat ccgtcttcag agagtgacgg
     3541 caaggaagaa ggaaccggca cggcgcgcag ccatgccggt ggatattaac ggctaaagaa
     3601 agcgcgctgg atcgccagtt cgacaccgcg aacttcggcc aggcctttca gacgaccaat
     3661 tgcggagtaa cctgggttgg ttttcttctt caggtcgtcc agcatctgat gaccgtggtc
     3721 cggacgcatc gggatcaggt cttctttgcc ttccgcttta cgacggtgtt cttcttcaac
     3781 aatcgctttc accacttcgt acatatcaac gtcaccgttc aggtgcgccg cttcgtggaa
     3841 ggttttcggg ttatcttcac gcatggtgga gcgcagatgg gtgaagtaaa tacgcggacc
     3901 gaactgcttg atcatatcaa ccagatcgtt gtcagcacgc acgccgtagg aaccggtgca
     3961 catggtaaaa ccgtttgcca tgctgtttac ggtatcaacc atccactgca tatcttcaat
     4021 ggtggaaaca atgcgcggca ggccgaggat cgggcgcggc ggatcgtccg ggtgaacagc
     4081 catacgcacg ccgacttctt cagcaactgg aataatcgct ttcaggaaga cggcaaagtt
     4141 ttcgcgcagc ttcgctttgt cgatatcttt gtacagctcc aggtgtttac ggaactggtc
     4201 gagggtgtag ccttcttccg cgcccggaag accagcaatg atgttacggg tcagacgcgc
     4261 tttgtcttca tcgctcatag tggcgaagcg aacagcagcc tgagcaattt cttcttcggt
     4321 gtaatccgct tccgcgcctg ggcgtttcag gatatgcatt tcgaatgcag cgaattcgat
     4381 ctggtcgaag cgcagagctt tggagccgtc tggcagcacg tattcgaggt cagtacgggt
     4441 ccagtcgagc accggcatga agttgtagca cacggtgcga atgccacact gcgccaggtt
     4501 gcgcagagtc tgctgatagt tagcgatcca ctgctcatag ttgccagtgt gggttttgat
     4561 atcttcgtga attggcacgc tttctacgac agaccacacc aggcctgcgt cttcaatgat
     4621 cgccttgcgt ttgaggatct cttcaacgga ccatacttcg ccgttcggga tatggtgcag
     4681 cgcggtaacc acgccagttg cgcccgcctg acggacatca gctaaagaaa ccggatcgtt
     4741 tgggccgtac cagcgccagg tctgttccat gatttttcct ctttcacata gtgggttcca
     4801 cagcaaagag acgcggaacc ctggagttgt ttaggtatcc ggatgaacaa ccagaccgca
     4861 gccgtggcgt tgcatcggat gagatggcgt ataagttcta ccttttggta agacatgtca
     4921 acccgaattc tgaaattggt taaccacatc acaagaattt cactctcatg gttgttgctg
     4981 caaattgcag caaagggtaa tcgatggtta ttgatcagag gatgtgacat tcatcgcaac
     5041 aatggttgac caatttacat aacatatcgc ccaaaataac aacggttcaa ccaggtaagg
     5101 taaaacgtta tgcatgtgct taacattctc tgggtggtat tcggcattgg tctgatgctg
     5161 gtactgaatt tgaagttcaa aatcaattca atggtggctt tgttggtggc ggcgctgtcc
     5221 gtcgggatgc tggcgggcat ggatttgatg tcgctgctgc acactatgaa agcgggcttc
     5281 ggcaacacgc tgggggaact ggctatcatc gtggtgttcg gtgcggtcat cggtaaattg
     5341 atggtcgact ccggcgcggc tcaccagata gcgcatacgc tgctggcgcg tctcggtctg
     5401 cgctatgtac agctgtcggt gattatcatc ggcctgattt ttggtctggc gatgttctat
     5461 gaagtggcct ttatcatgtt agcgccgctg gttattgtta ttgccgccga agctaaaatt
     5521 ccgttcctga aactggcgat cccggcagta gcagctgcca ctaccgcaca ttcactgttc
     5581 ccaccgcagc cgggtccggt ggcgctggtg aatgcttatg gcgcggatat ggggatggtt
     5641 tatatctatg gcgtactggt gacgatccca agtgtaatct gcgcaggtct gatcctgccg
     5701 aagttcctcg gcaatcttga gcgcccaacg ccatcattcc tgaaagcaga tcaaccggta
     5761 gatatgaata atctgccctc tttcggcgtt tcgattctgg tgccgctgat cccggcgatc
     5821 attatgatct ctaccaccat cgccaatatc tggctggtaa aagatacccc tgcctgggaa
     5881 gtggttaact ttatcggttc ctcgccgatt gcaatgttta ttgcgatggt ggttgcattc
     5941 gtactctttg gcaccgcacg tggtcatgac atgcagtggg tgatgaacgc ttttgaaagc
     6001 gcggtgaaga gtattgcaat ggtgattctg atcatcggtg cgggtggcgt gctgaagcag
     6061 accatcatcg acaccggcat tggcgacacc atcggcatgt tgatgtccca cggcaatatc
     6121 tcgccctaca tcatggcatg gctgatcact gtgctaattc gtctggcgac gggtcagggt
     6181 gtcgtttcgg cgatgaccgc cgccgggatt atcagtgctg caatcctaga tccagcaact
     6241 ggtcagctgg ttggcgtgaa tccggcgctg ctggtactgg cgacggctgc gggttccaac
     6301 accctcaccc acattaacga tgcatctttc tggttgttca aaggttactt tgacctgtca
     6361 gtaaaagaca cgttgaaaac ctggggtctg ctggagctgg tcaactccgt ggttgggctg
     6421 attattgtgc tgattattag catggtagcg taaaaggaca ccagagcctg ccaatggcag
     6481 gctcagactg atgacaaagt ctaaaaatgt gcccggacag tcccctcgcc cctccgggga
     6541 gagggttagg gtgaggggaa cagaccagca aagtgcgctt tgtttatgcc agatgcgacg
     6601 ctgacgcgtc ttatctggcc tacaaagggc taacgtgcag gtttttagct tcaggtaata
     6661 ttgcgtacca gcattagcaa tgtcctgtga tttctttatt gataaacaaa agtcacgcca
     6721 ataatcgatt gcacattccc tgcagtcacc tgccctccgg tacgtgcata atttgccgtt
     6781 aatcccagac tcaccgccga agtccctact gctcctaacg ataccgtgtt attcgctgga
     6841 ataatcgtac cgttgcgcgt caactgtacg ccgacgccct gtgcaggtga aaacgacgcg
     6901 gtattggtga aaatcgagtt gcccgcatct gcggttgtgc cggagaggta ataccccagg
     6961 ttttggcttt tcgcacaata aacggtaaga ggaattggca ctgaaccagg gtagtccggc
     7021 agagtaacgg tgacatcacg agcagaaaca tcgcagccgc cagtaggcac caccacatca
     7081 ttattggcgt aaatattcca cacaaactgg aaatcatcgc tgttatagtt gttggtctgt
     7141 cgcaaaataa gcacggcaat taatgagcca gctttaatcg ccaccccgcc cgcactgctc
     7201 acaggcgtca aataaagcgc caccggccac ggcttatccg ttctcgaatt ataaacaacg
     7261 cgcggcgttt cgctggtggt aggaaatgga tagctactgc cactatattt tacggtcccg
     7321 gaaaaattag ataacacgcc gccataagcc gagcctcgtt gcagtgtgac atagtctgta
     7381 atggtttccg gataatcgtt atggcaaaag atttgcgtcg aaagatccac gaccaggttt
     7441 tgccccacat tcacgacggg cgcaaggttt acataaacat tggcgctgcc accgccaata
     7501 gggatagcgg taccattggc ggttttacag gcgaatgacc aggcatttac cgaccagccc
     7561 atcagcagta cagcaaacag ggtaataact cgtttcatta caatcatctc ttcgggttca
     7621 gctgtaggta taggtgatgc taatcactgc ctgaatggtt ccctgagtgg ctccgccatt
     7681 tactgtcaat gctctgacct gtaacgggaa gtgcgctgat tgtgaggaat catccacctg
     7741 aactgttttg gttgcgccag tattcaatgt gttgccactg tcatcctgta gctctaactg
     7801 gatgttttgc gcggtcccct ggtttttata atatccggta ctgtcggctg ccccgctgaa
     7861 gctggcagtg accctcgacg ttcccaccgg acaattagtc aactcaagcg caacatcatg
     7921 ccaggccgat gccgccccgg cagacataag actgaaagaa taaagatcgc cgagatcaac
     7981 cgtggcattg gtggtggaaa ccgtacacgg tttggcgacg accttaccgt tcaccgtgat
     8041 ggtgacatcg gctgcctgta tcgtcgcact tgcgagcgcc aatattgccg ccaatacata
     8101 cccacgtttg caccatttca tgagcatctc cagttactga tattcaagag tgaaggtagc
     8161 cgtggcattg atatgccccg cagtgacagg cacctgtgtc gccattagcc gggcgtaaaa
     8221 attcagcgta tttggtttac ccggcgtcag ggtcgtccac gaaagcgcgg acgatggagc
     8281 attaaggggt atttgatttt gctgctcatt cagaagctgt attcccagtc ccgaagccgc
     8341 tgacaccgta ttttcaagtg caagcaggtt ggcattgtgg ctatctgcaa cgccagtaaa
     8401 cccaaccttt acggcagaaa cggcattacc acagggtgac agcaaaatac gaaatggaac
     8461 aacaggagtc gtcgcgccaa tgttgttaaa ttgcttcgcc gcgttttcca tcagatcaac
     8521 agtaaaattg gttgattcag cggccacact acagccgtta tccctgacat agccgcggat
     8581 agtaatcgtg ctatccgcag ccaaagcgtg actcaccgcc agccacaaaa aagcgcacag
     8641 aagataaaaa ggtttgtttc tcatcacgcc cccttaacga cattcagctg atagctgggt
     8701 taataactgc tgctgactct ctggtggcag ttgataattg gcgacacagt gagcattttc
     8761 ctcttctccc catttcacct gaacttttcc cgctaaaggc attccgctga ggtaaacctg
     8821 accattatcc gcaacaatgc cgctactctg gctactctct gatgtcacca tcgccccaaa
     8881 cggcagcggc ttattattgt gggtcagcgt catgagcagt tttatcccaa cgcgcgcttt
     8941 aaactctgct cgcacgatcg ccccacgagt gggaacaacg ttagcaaccg cgttatctaa
     9001 atcgacgtta tcagccaggg tattggtatc cagcgccact ctattttccc gatattcagt
     9061 ggcataaggc agcacggcat aaccacgcca gtcggtacgc acccccgtct ggttttcgac
     9121 ttttgcatct tttgcgccag gcgctttaac aagcaccacc gtatcgttta acggctgccc
     9181 cagcgttacg ccattggcat gagccagtac cccaccgctg actccgtaat agagctgctt
     9241 aatatcatcg ctatggctgt aaccgatatt ggcattgccg taaccaccgc gataattcag
     9301 cgtggcgtag cctgtacttc cgctatttcc atcgcctccc ccggcatagc cggtttgcac
     9361 gctatagctg aggttgttgt cttccagcaa cgtaccgtat acaccagcca gattggtcat
     9421 ccgaccgttg agatcgtgtg acatgctgta gctggcactg gcatgtcgcc actgagattt
     9481 actgtcagaa cgcagccagt ggctgaaagg aatattgacg ttaagcgcta acatctgatc
     9541 ccgtcctttt tgccaggcgt ttttcgtcag gctatagctg agcgtccagt tgatatcttc
     9601 gaacgcagta tttaatccag cctggaattg ctcatcgaca ttactcgttc cccaataagt
     9661 ttgatggcta ccactcaaat acagtgttga tgtgcgcccg agttgctgag taacggtgag
     9721 ttgtaatttc ccgcgtttgt tataagcgag gttgtaatag tcggtgaatt tcggcttaac
     9781 ctgaataact ccgtcctgtg tttcgatgtt gtagccattc attcgactgt atgttgtatc
     9841 agcgaaatta aaatatccgc tggtcgaata acggtaaccc actaactgaa tattcgtgcc
     9901 tgattcattg agcgatttgt tatagagaaa acgcaccgat tgtccgtcat gctgactgtc
     9961 atcgggaagt gtggaattag cctgcgtcat atccacagac agagcgccca gtgcccccat
    10021 gtttttcccg ataccgaaat taaaagcacg ataacgatcc gccagttgcg ttccaccata
    10081 tattgtccag ccagccggaa ggccgtggag taatgtactc tggaaaaagc ggggtttttc
    10141 ctgctgcgca tttccactac ggtattctcc tgccgtaatg gaataacgag tatgcccttc
    10201 acgttgcaaa agcgggactg acgaataggg tacggtaaaa atctgcgtgc tgccgtcagc
    10261 ctctttgatc gttacctgca agtcaccact attacctgcg gcatagatat cgttgatggt
    10321 aaaaggcccc ggtggcaccg tactattata aatgtcatac ccattttgtt taatagtgac
    10381 ctgtgcagta ccacgagcaa taccgtggat caccggggca aatcctcttt gactatcggg
    10441 taacatattg tcatctgagg ccaattgtgc gccgcgaaag ttaataccat cgaaaatatc
    10501 gccctgagta taaccatcac ccagcgtcag ccggaaacgt aacggtatta tgtctcgctc
    10561 aagccaggta ttgatatgct gccatttatt tttgctacct gatgatctgt cgctactttt
    10621 ataactccag gtggtattgt cgcgtaaacg ccacgcacca atatttaacc cactctgtag
    10681 gtttaaatat gcataatggc tgttaccccc aatccgattc tgtacactat ttccgctgaa
    10741 attataattg agcaatccgg cattaatacc gggatcccat aactcaggag gaatataacc
    10801 acgcgcgcga ttactcataa atgcctgagg gatcgtcagg ttcagtcgct gctgaccaac
    10861 atccagatgc gcagtagcgt cctggaccat tgtggttaat ggcacacagg catcatccgc
    10921 cagcagattc ataccggcga cagaagccgt attcagcccc atactggcga gttgcgcgcg
    10981 tgtcaggcag ggaacaatcc cttgttcact gtcgcccgta ttaaatgtga catcacgcgt
    11041 tgccatataa ccattattca aatagatatc gacgcgatac gtccctggcg gtaattcttg
    11101 cccattttca aaacgcgata aatcggccac agcctgggga tcatccgcta aaaagcgcgg
    11161 attaaaatag aggtcggcag atgacaaagg tgcctgtgcg gcaaaagcac aggcgacaac
    11221 gagccggata aaaaaaacag ccaaacgatg cttacgaata tgcaagcatt gtgtgtttcg
    11281 ctggtaaagt cttaaattca gatatgacat tattcctccg gcttaaaatc acccggcagt
    11341 caattctttt tattcaggcg aaaaattccc cctgcgttat tccattacgc ccgtcatttt
    11401 gggggtaagt gcgccataat catttattgt tcggtaagta atattgcttc ctgcatcaga
    11461 aggcaattta accgtgcttt cgcccattgg aggcaccaat gcattttcaa gaacccgggt
    11521 tccggcattc aactctgtta ccgtcaggta atagggtgtc gggttaatca gcgtcagaga
    11581 attcgcgcta cgacgaaatc ttaatttttc tgcggcctga tcgggtggca acgctaattt
    11641 agccgggcga tagtacagtt taatgcggct gataattgcg agctgtagcg tattctcagt
    11701 caattttgat ttatccattg acggaatcgc tttaacgttc atccagaata aactttcccg
    11761 gtcctgtggc aattggttat ttgttgcatc aagaatacgt aaggtattct ctttttttcc
    11821 cttcatcgca aacagaggag gcgtcacgat aaaacgacca tcctttacac catcggcatt
    11881 ttccacccat gattgaatta aataggtact attttcatca ttatttgtca cggcaagttg
    11941 cacttgtttt tgccctgccg gataaattac gcgagtcgca cctaaggcca ctcctgcttc
    12001 agcgcgtccg gcaaccatca ttgccatgaa catcaggata cctgccagca agcagaatgt
    12061 tatttcctgc gatttcctta cattgacgtt tttattactc actgtcctgt tcctgttatc
    12121 acattatctg ctgaacaatt actgataggt taaagagaac caggcctggg cattggcgat
    12181 gccgccagta acccgacgcc cggtagcacg atatttggcg atgaaatgta gcgaagtaga
    12241 gcctgaataa agccgtttcc agtttgctgg aggacgatta atcggtacga ggtttccttc
    12301 atcatcaaac aacgctacgc caatattggt ggctatccct ggcccctctc ccacggaaag
    12361 cacatccgga tttttaccat ccgcgacacc gtgaaacgcc acacctacac gttcactcac
    12421 caccgtgcta cattcccgta aatgaataac aaaaggcacc ggtgcgctat cttccccaac
    12481 cgcatgaaac cggttactgc tgatttgccc catattgacc gtcatttgtt tatcaccggc
    12541 ttcaatccgg caagtttccg caataatgac gccctgaaat tgcatatttc cgccgggcaa
    12601 cgtggtattc catttatttc cggccagagc aaacattggc agcaacgacg ctaatagaaa
    12661 taatctcttc cttttcatcg ttatttttat cgcacaaggg tgggcatccc tgcccgtaat
    12721 gacgtccctg aacctgggta ggttattgat actgaacctt gaaggtcgca tccgcattag
    12781 cagcacccgg ggttgcggcc ccggttgcaa aataacgcgc ctggaacgga atggtattgg
    12841 ttccgttatt cagggttgtt tctgaactaa atgtcgcacc atccagcgtc agcgcagcac
    12901 ccgttctgtc caggatctgc acaccaacgt ttgttgcgct acccgcagct gaactctgca
    12961 gagccagaac gttggtatga cccgcatcaa tcgccgtacc taaaaaggca acagcggctt
    13021 tagatgcaac attggtatcg caatcattca gctgaatgtt aaaaccgaca gcagaactgg
    13081 ttgctccttc ctgtgccagc gatgcggtac gaacctgtcc taactgaacg gtttgatcaa
    13141 cagagcctgc atcaactgcg caagcggcgt taacaacttc ccctttaaag tgaacggtcc
    13201 caccattaac cgtcgtggca gcggccagag ccgctgtaga actgagggac agagccgaca
    13261 gaacaacgat tgccagagtt ttaattttca tgctgctttc ctttcaaaaa actatttcta
    13321 aatcgacatg ggcagtcgtt ctgtacactt tgttttgtca acgagtttga ctgccaacac
    13381 tgcacagttt tctcccaaaa gatgaaacat ttggggccat tttgactcat agaggaaagc
    13441 atcgcggaca aactttttca gtttatttgt tggcttaata ttctattgtt atctttattt
    13501 atagatgttt atattgcatg aggtggtttt tggagagaag aatgaggaag atgcgtcgag
    13561 ccacagaaac gttagcttta catatagcgg aggtgatgtg aaattaattt acaatagaaa
    13621 taatttacat atcaaacagt tagatgcttt ttgtcgtttt ttaatatttt tatgcttgag
    13681 aaaaatacgt aacttattta tgatatggac agtttggccc caattgtctt gtatttattt
    13741 gtttttttaa ctttattatc aataagttaa atcaaacctc ttctcttttt aatttttcgt
    13801 ttatgagatt atttctttcc cataatccgg caaaacgagc agcattactg gcggtataac
    13861 gcacagtatg gcgaatattt cgatgcccga gataatcctg aattaaacga gtatctgcac
    13921 cacgctccgc caattcataa ccgcaagcat gccttaacat atgaggatga gtctgcgtta
    13981 cggttccagc ttcaataccg gcatcgcgaa taatgcgata ggcctgctgg cgagaaagcc
    14041 gactcccgcg gcgagaaata aatatagcgt cagtccggtc agcgcctttc cagttagcac
    14101 gttcctgggt ccagcgttcc acggcttcac gctcatcaaa acgtaacggg tgaacggtag
    14161 aaaatccgtt cttcagtcgg cgaatattta ttctaccttc attaaggtca aggtcctgat
    14221 aatgcagatc aagcagttca ctaatacgca tcccatgtcg atatgccaac agaataagac
    14281 aataatctct ggctcccgtt gccccgtaac aaaccgcctg catcatggcc tgaacttctt
    14341 taccggtaag ataacgacgt ttactcacaa tagtagtact cctaactgag atatatttga
    14401 atgaatacct ataggaaacc tcaatcggtc aaaattagcc caatagataa aaagataacc
    14461 acacacggag tgatgtggct atcagtcaat tacattaaat atcatacaaa aataaaatat
    14521 cactgatgtg acaacatcta ttttcattga tttgattcaa tataacaat
//
LOCUS       DAJOWB010000115        14548 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJOWB010000115 DAJOWB010000000
VERSION     DAJOWB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.115"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(17..169)
                     /gene="hokE"
                     /locus_tag="OHO85_003765"
     CDS             complement(17..169)
                     /gene="hokE"
                     /locus_tag="OHO85_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCQ2673084.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            622..1740
                     /locus_tag="OHO85_003766"
     CDS             622..1740
                     /locus_tag="OHO85_003766"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCQ2673085.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAATDHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHIHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1806..2054
                     /locus_tag="OHO85_003767"
     CDS             1806..2054
                     /locus_tag="OHO85_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCQ2673086.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVSTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2119..2487
                     /locus_tag="OHO85_003768"
     CDS             2119..2487
                     /locus_tag="OHO85_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCQ2673087.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2581..3234
                     /gene="nfsB"
                     /locus_tag="OHO85_003769"
     CDS             2581..3234
                     /gene="nfsB"
                     /locus_tag="OHO85_003769"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCQ2673088.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAADNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3342..4589
                     /gene="ybdG"
                     /locus_tag="OHO85_003770"
     CDS             3342..4589
                     /gene="ybdG"
                     /locus_tag="OHO85_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCQ2673089.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4657..6033)
                     /gene="pheP"
                     /locus_tag="OHO85_003771"
     CDS             complement(4657..6033)
                     /gene="pheP"
                     /locus_tag="OHO85_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCQ2673090.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6135..9278)
                     /gene="cusA"
                     /locus_tag="OHO85_003772"
     CDS             complement(6135..9278)
                     /gene="cusA"
                     /locus_tag="OHO85_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCQ2673091.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAVPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9290..10513)
                     /gene="cusB"
                     /locus_tag="OHO85_003773"
     CDS             complement(9290..10513)
                     /gene="cusB"
                     /locus_tag="OHO85_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCQ2673092.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWVAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10529..10861)
                     /gene="cusF"
                     /locus_tag="OHO85_003774"
     CDS             complement(10529..10861)
                     /gene="cusF"
                     /locus_tag="OHO85_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCQ2673093.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGEV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10885..12267)
                     /gene="cusC"
                     /locus_tag="OHO85_003775"
     CDS             complement(10885..12267)
                     /gene="cusC"
                     /locus_tag="OHO85_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCQ2673094.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12424..13107
                     /gene="cusR"
                     /locus_tag="OHO85_003776"
     CDS             12424..13107
                     /gene="cusR"
                     /locus_tag="OHO85_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCQ2673095.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            13097..14545
                     /gene="cusS"
                     /locus_tag="OHO85_003777"
     CDS             13097..14545
                     /gene="cusS"
                     /locus_tag="OHO85_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCQ2673096.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         3483 a         3955 c         3600 g         3510 t
ORIGIN      
        1 gctccccgca caacggctac ttcttcggtt cgtaagcgag aacagcctta aactcaatat
       61 tacgttcctt tacggtgaac tcacacagcg aatctccgac cagaagcgta aatcccagca
      121 ccgttaaaca cagcactatg actgccgcaa gggcatattt cgtcagcatg tttatattgc
      181 ctccatgcga agaagggact accatccaaa ttgttcgggt ttggagtggc agccccaggg
      241 ttgatagaaa tatctcctgg ggcttcatct ttccagacct cagagtaaac ctgaaaccgg
      301 aaagcttcag gcacccgccg ttatcttacc cttcctcaac aaaatagctg tttatttata
      361 cagtgagtcc tgtttcggac ttctcactcc tcatttaatc aactgaagct gaacccgttt
      421 ttttctgcat gactcaatcg tcaaaattct tcgccaccag tcagcactgc cataacttgt
      481 cacattgcaa aagtagccaa attgcttcag gcaccatcag ttcaccatca ccaaaaaaaa
      541 gaaacattcc agaacttccg catctcgccc agccgctccg acttctatac tgaatagaaa
      601 acgccaacat aagagaaacc tatgccatta cccgattttc atgtttctga accttttacc
      661 ctcggtattg aactggaaat gcaggtggtt aatccgccgg gctatgactt aagccaggac
      721 tcttcaatgc tgattgacgc ggttaaaaat aagatcacgg ccggagaggt aaagcacgat
      781 atcaccgaaa gtatgctgga gctggcgacg gatgtttgcc gtgatatcaa ccaggctgcc
      841 gggcagtttt cagcgatgca gaaagtcgta ttgcaggcag ccacagacca tcatctggaa
      901 atttgcggcg gtggcacgca cccgtttcag aaatggcagc gtcaggaggt atgcgataac
      961 gaacgctatc aacgcacgct ggaaaacttt ggttatctca ttcagcaggc gaccgttttt
     1021 ggtcagcata tccatgttgg ctgcgccagt ggcgatgacg ccatttattt gctgcacggc
     1081 ttgtcacgat ttgtgccgca ctttatcgcc ctttccgccg cgtcgccata tatgcaggga
     1141 acggatacgc gttttgcctc ctcacgaccg aatatttttt ccgcctttcc tgataatggc
     1201 ccgatgccgt gggtcagtaa ctggcaacaa tttgaagccc tatttcgctg tttgagttac
     1261 accacgatga tcgacagcat taaagatctg cactgggata ttcgccccag tcctcatttt
     1321 ggcacggtgg aggttcgggt gatggatacc ccgttaaccc ttagccacgc ggtaaatatg
     1381 gcgggattaa ttcaggccac cgcccactgg ttactgacgg aacgcccgtt caaacatcag
     1441 gagaaagatt acctgctgta taaattcaac cgtttccagg cctgtcgcta tgggctggaa
     1501 ggcgtcatta ccgatccgca cactggtgat cgtcgaccgc taacggaaga taccttgcga
     1561 ttgctggaaa aaatcgcccc ttccgcacat aaaattggcg catcgagcgc aattgaagca
     1621 ctgcatcgcc aggtcgtcag cggtctgaat gaagcgcagc tgatgcgcga tttcgtcgcc
     1681 gatggcggct cgctgattgg gctggtgaaa aagcattgtg agatctgggc cggtgactaa
     1741 gtcgggaatt gccttacaga agaatatccc tgacaatggt gttttaactt acatttaaca
     1801 atgttatgaa acacccttta gaaaccttga ccaccgcagc aggcattttg ctgatggctt
     1861 tcctctcttg cctgctgctt cccgcccccg cattggggct gacgctggca caaaaactgg
     1921 tgagcacgtt ccatctgatg gatcttagtc agctttacac tttattgttt tgtctgtggt
     1981 ttttagtgct gggcgctatt gagtattttg ttctgcgctt tatctggcga cgctggttct
     2041 cgctggcgga ttaaacgtcg attatcgccg tacttcgcgg catactttgc ttattctcct
     2101 tcgcctaaag gaacgtttat ggataagcaa tcactgcacg aaacggcgaa acgcctggcc
     2161 cttgagttac cctttgtcga gctttgctgg ccttttggcc cggagttcga tgtttttaaa
     2221 attggcggca agatttttat gctgtcgtcg gagctacgcg gcgtcccctt tatcaatctg
     2281 aagtccgatc cacaaaaatc cctgttaaat cagcaaatat acccaagcat taagccaggg
     2341 tatcacatga ataaaaagca ctggatttcg gtgtatcccg gcgaggaaat ctccgaagcg
     2401 ttacttcgcg atctgatcaa cgattcgtgg aatctggtgg ttgatggtct ggctaaacgc
     2461 gatcaaaaaa gagtgcgtcc aggctaaagc ggaaatctat agcgcatttt tctcgcttac
     2521 catttctcgt tgaaccttgt aatctgctgg cacgcaaaat tactttcaca tggagtcttt
     2581 atggatatca tttctgtcgc cttaaagcgt cattccacta aggcatttga tgccagtaaa
     2641 aaacttaccc cggaacaggc cgagcagatc aaaactctcc tgcaatacag cccatccagc
     2701 accaactccc agccgtggca ttttattgtt gccagcacgg aagaaggtaa agcgcgtgtt
     2761 gccaaatccg ctgccgataa ttatgtgttc aacgaacgta aaatacttga tgcctcgcac
     2821 gtcgtggtgt tctgtgcaaa aaccgcgatg gacgatgcct ggctgaagct ggttgttgac
     2881 caggaagatg ctgatggccg ctttgccacg ccggaagcga aagccgcgaa cgataaaggt
     2941 cgcaagttct tcgctgatat gcaccgtaaa gatctgcatg atgatgcaga gtggatggca
     3001 aaacaggttt atctcaacgt cggtaacttc ctgctcggcg tggcggcgct gggtctggac
     3061 gcggtaccca tcgaaggttt tgacgccgcc atcctcgatg cagaatttgg tctgaaagag
     3121 aaaggctaca ccagtctggt ggttgttccg gtaggtcatc acagcgttga agattttaac
     3181 gctacgctgc cgaaatcacg tctgccgcaa aacatcacct taaccgaagt gtaattctct
     3241 cttgccgggc atctgcccgg ctatttcccc tcagattctc ctgatttgca taaccctgtt
     3301 tcagccgtca tcataggctg ctgttgtata aaggagacgt tatgcaggat ttaatatccc
     3361 aggttgaaga tttagcgggt attgagatcg atcacaccac ctcgatggtg atgattttcg
     3421 gtattatttt tctgaccgcc gtcgtggtgc atattatttt gcattgggtg gtactgcgga
     3481 ccttcgaaaa acgtgccatc gccagttcac ggctttggtt gcaaatcatt acccagaata
     3541 aactcttcca ccgtttagct tttaccctgc aggggattat cgtcaatatt caggcggtat
     3601 tctggctgca aaaaggcacc gaagcggcag atattctgac tacctgcgcg cagttgtgga
     3661 tcatgatgta tgcgctgctt tcagtcttct cgttgctgga tgttattttg aatctggcgc
     3721 agaaattccc ggcggcatct cagttaccgc tgaaagggat atttcagggg attaaactga
     3781 tcggcgcaat cctggtcggc attttgatga tctcgctgct gattggtcag tcgccagcga
     3841 ttctgatcag cggtcttggt gcaatggctg ccgtgctgat gttggttttt aaagatccga
     3901 ttcttggtct ggtggcaggt attcagcttt ccgcgaacga tatgctgaaa ctgggcgact
     3961 ggctggagat gccgaaatac ggcgcggatg gcgcggtgat cgatattggg ttaaccaccg
     4021 tcaaagtgcg taactgggac aataccatta ccactattcc cacctggtct ctggtttctg
     4081 actcctttaa aaactggagc gggatgtcag catctggcgg gcgacgtatt aagcgcagta
     4141 tcagtattga tgtcaccagt attcgttttc ttgatgaaga cgaaatgcaa cgtctgaata
     4201 aagcgcactt gttaaaacct tatttaacca gccgccatca ggaaattaat gagtggaatc
     4261 gccagcaagg ttctacggag tcgatattaa acctgcgccg aatgaccaat attggaacct
     4321 ttcgtgccta tctgaacgaa tatctgcgta accatccgcg gattcgtaaa gatatgacct
     4381 taatggtacg ccaactggct ccaggtgata acggtttacc gctcgagatc tacgcgttta
     4441 ctaacaccgt ggtgtggctg gaatatgaaa gcattcaggc tgatatattc gatcacatat
     4501 ttgcgattgt cgaagagttt ggtctgcgac ttcatcagtc gccaaccggc aatgatattc
     4561 gctctctggc gggtgcattt aagcagtaat taaaaaaacc gctctcatcg aatggatgag
     4621 agcggacatc gttttgtcgg atgtagcgtg aatgccttat ttccgacgca gcgttttaaa
     4681 tgccacaaac aggaatacaa tccacaccgg cagcaggatc gctgacaagc gcatatcatc
     4741 catcgtgcac atcagcagca aaatcatgcc gaggaaggca atgcaaagat agttgccgaa
     4801 cggatagagc agcgccttaa actgtgtttc acgcccctga cgtcgcatcg ctgcacgaaa
     4861 acgcagatgc gccagacaga tcataatcca gttcaacagc agcgttgcta ccaccagcgc
     4921 catcagcaga ccaaacgctt tttgcggcag cagatagttg attaatacca ccagcgaagt
     4981 gatcgctccg gaaagcatca gcgagttaat cggcacaccg cgacggctga cgcgagtcaa
     5041 aaacttcggc gcattaccct gcacagaaag gccaaacagc atgcggctgt tagagtaaac
     5101 cccgctgtta tacactgaca gcgatgctac cagaatgacg aagttcagcg cagaagctac
     5161 cacgttgctg tcgagattat ggaaaatcat cacaaacggg ctactgttgg atttcacttc
     5221 cacccacgga tagagcgcca gtaaaaccac cagtgaaccg atgtaaaaca gcaggatgcg
     5281 atacaccacc tgatttaccg cttttggaat gcttttttcc ggatcgcgcg cttcagcggc
     5341 agtaatccca atcagctcca gaccgccgaa ggagaacata attaccgcca gcgacaaaat
     5401 cagcccattc cagccggtgg caaagaaacc accgtagcgc cagaggttgt cgatactggc
     5461 tttctcgccg ccgtgaccag aaaacagcag ccacaggcca aagccgatca taccgatgat
     5521 cgccagcact ttaatcagcg caaaccagaa ctcggtttca ccatataagc gcacgttcac
     5581 caggttaacg gcgttgatga taataaagaa ggcggcagcc caaatccacg ttggaacatc
     5641 cgggaaccag tactgcatat agatgcccgc agcggtcagc tctgccattc ccaccagcac
     5701 gaacattacc cagtagttcc agccagagag gaagcccgca aacggtcccc agtatttata
     5761 ggcaaagtgg gcaaatgaac cggataccgg ctcctcaacc accatttcgc caagctggcg
     5821 cataatcagg aaagcgatga tcccggcgac gccgtagccc agcaatacag ccggacccgc
     5881 catctgaatc gccgggccaa tgccaagaaa cagaccagta ccaattgcgc cacccagcgc
     5941 aatcagttga atatgacggt tatgtaatcc gcgatgaagc gtcggctctt gattcgacgc
     6001 agtatcttcc gatacggttg acgcgttttt cacgcctttc ccctgtgtgt cttttttgtt
     6061 gaggggcacc ttttaacatt tagtgcccat cgtcgcaaga cacaatccac acggttaaac
     6121 ggggtatcct gcttttattt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     6181 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggcgcg
     6241 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6301 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatat
     6361 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggcacggcc
     6421 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggcg
     6481 agggcgataa agccagtgcc cgtcgccacg gaaagatgaa agcccatcca ccacagcaac
     6541 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6601 cgacggaacg ccagatacaa cagcacgaag ataatcatca acgtcatcgg caccatgagt
     6661 ttaagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6721 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     6781 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     6841 agcatcgacg gtccggtaga gactttaatg tcggccacgt ctgccagggt gatttgctgc
     6901 ttcatcggcg tcaggatcgg cagctggcgc agtgcctgcg gactatcgcg ccagctttgc
     6961 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     7021 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     7081 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcggcaagc
     7141 gcagaagcta cgcctggcac cgttcgcgct acttcttcaa tttgctcagc catcgcgtcg
     7201 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7261 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagccgc
     7321 acggtgttat ccagttcctc aatgattttg tccatcgtca tgcctggccg ccactgctcc
     7381 tgcggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7441 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7501 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7561 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7621 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7681 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cgggttactg
     7741 ctttccggcg gaattttgcc acggatccag tagcccatca ggatcgggat cactacgatc
     7801 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     7861 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     7921 ctgataaata gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     7981 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagccg tttatgcgca
     8041 ttctcgatca tgacgatagc agcatcgacc atcgccccga cggcaatcgc aatgccaccc
     8101 aacgacataa tattggcatt cagtccctgg aagtgcatga caataaaagc aatacacaac
     8161 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacagcgca
     8221 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcg
     8281 cggtcaatga gctggctgcg atcgtatgtt gtaactatct ccacgccttc cggcagacta
     8341 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8401 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttagttc ggcaatgccc
     8461 cggcgcatct ccgggccaat ctggaccttc gcaacatcgc gcagataaac gggcacgcca
     8521 ttttcactgg cttttaaaac gatgtgatta aagtcgtcga gcgtttgcag atagccgctg
     8581 gcgcgcacca tatattccgc ttccgccagt tcgatcgacg aaccgcccgc ttcctggttt
     8641 gaagcatcca gcgcgctttt tacttcggcg agactgatgc catactgcgc caggcgctgg
     8701 ggatcgataa ccacctgata ctctttcacc acaccgccca ccgacgccac ttccgcaacg
     8761 tcagggatgg ttttcagctc atatttgaga aaccagtcct gtaatgagcg taaatcggcc
     8821 agatcgtgct taccgctgcg atccaccagt gcatattcat agatccagcc aacacccgtg
     8881 gcatctggcc ccagctcggc gctgactccc gcaggcagct taccctgtac ctggttgaga
     8941 tactccagca cccgcgaacg cgcccagtac ggatcggtgc catcttcgaa aatgacatac
     9001 acataagagt cgccaaactg tgagaaaccg cgcacagtct tcgcgccagg caccgacaac
     9061 atggtggtgg ttagcggata agtcacctga ttttcaacga tttgtggtgc ctgaccggga
     9121 tagctggttt taataataac ctgcacatcg gagagatccg gcagcgcatc cactggcgta
     9181 ttaatgatgg tccaggtgcc ccagatgctc agaaacaacg cgcccatcag caccaaaaaa
     9241 cggttcgcca ccgagcgacg aataatccat tcaatcattg gttattccct caatgcgcat
     9301 gggtagcact ttcagagcgc atccgctcca gtgcgccaga aatattggct tcggaatcaa
     9361 tcaggaacag gccgctggaa accacctttt caccttccgc cagaccagag cgtaatgcgg
     9421 tgacgccttg cgatgcctgg aaaacagcaa cgcgtttcgg tacaaagcgc ccgtcggcat
     9481 caacggtaat cacccgctgt tcgctgccgg tatcaatcag cgcttgtgac ggaatgagca
     9541 gcatcggttc gctggcggtg ttgagttgca accaggcgtt catgcccggt tttagcgcct
     9601 cgtcggcgtt gtcgacttcc agacgcagct gcagcgtgcg ggtcgcggca tccacgccag
     9661 gtagcagcgt ccatttgcgg atggtgagtg ttttatccgg tcgcgccgga acggtgaggg
     9721 taaactgcga ggcatctttc accagccagg cgatggactc cgggatcgca gcagtgaccc
     9781 acaccgggtc cataccctga attttcgcga ccacgttatc tttggcgata ttcattcccg
     9841 cgcgcagatc aaacgcggtg atcacgccat caatgggcgc tttgagcgta aagcgagtct
     9901 ggattttttg cgtggcgatc aggcggcgaa tatccgcctc cggcattccc gccagtcgca
     9961 gccgctcaag aatgccttca gtctgggtcg ccgtaccgcc ggtttcgcgc agcagtaaat
    10021 actcactctg cgcttccacc cagtcaggaa tggtcaggtc gagaagcggt gtgccctttt
    10081 gtactttatc gcccacggta agcggataca ccttgtcgat aaacccggca gcgcgagcct
    10141 gcacaatggc atactgatac tcgttgtaac tgacattcgc cgggaaactc tgggcaaaag
    10201 tcagcggtcc gcgcgtgacg gtagccgttt tcacccccag attctgcgtc tgagtcgggt
    10261 caatgcgcac accagacgca gaactctctt catcggcata tttcggcacc agatccatat
    10321 ccataaacgg cgatttacct ggtttatcga accgcgtatt gggatacatt gggtcgtacc
    10381 agaataagat tttacgttct gcggtcgacg ttttttctgc aggcggttcc gcctttgcaa
    10441 cccaggtaaa gcctgccgca gaaataatac cgcccgcgat catgctgccg ataataagcg
    10501 cgattttttt catctcatta aacctgggtt actggctgac tttaatatcc tgtaataaag
    10561 aaaggttgcc ctgctggaca aaattaaacg ccactttgtc gccggtttta atttcactca
    10621 ttttcgtctg cggggtgatg gtaaagcgca tggtcatctc cggccagttc acagcagcaa
    10681 tcggatcgtg atggatggtg atttttttgc tttccagatc aatacccttt acctcgccag
    10741 tggcactaat aacctgtggt tgtgcttcgc tcatggtttc atgatgatgt tcgttagcct
    10801 gggcattaaa gccaataacg gtaaacagac tgaacattgc gacttgcagt gcttttttca
    10861 tttattctct cctggagtta aaagttattg ctgccaaccg ccaccaagtg cggtatataa
    10921 agaaatttcg ttaacctgac gggcataatt cagatcaagt aaagtttgtc gggttgcaaa
    10981 taaagaacgc tcggcatcca gcacttccag ataacttact gcgccgtgct gatataatgc
    11041 ccgcgcccgt tgcaaagtaa tttgcagcga tgccagataa cgctgctggg cgctaatttg
    11101 atcgttcagg ctttgacgta atgccagcgc atctgccact tctttaaagg cgttctggat
    11161 tttctgttca taattcacca ccgactgctg ctggcgaatt tcggcgatat ccagattggc
    11221 ctggttgcgt ccggcattaa aaatggggat ctcaattttg ggtataaaat tccacatccc
    11281 gctgctggcg ttaaataatg acgatagatc gctactggcg gtcgatattc cgctggtcag
    11341 gcttatagac ggaaaaaatg ccgcgcgtgc agcgccaata ttggcattag ccgccattaa
    11401 cgcgtgttca gcttccataa tatccgggcg ctgcaataag atttgcgacg acaatcccgc
    11461 cggtaattta acgctttgca ggctgtcgct gtttactgtc tgcgcttgcg gcagcttgcc
    11521 gtagcttccc agcaacagtt gcaatgcatt attcgcctgc gccagttccc cctgacgttt
    11581 agcgatgtcg ctgcgggtac tttctatcac cccgcgagcc tgttccagcg ccagaacatt
    11641 actgctacct gtcaacagtt gtttttcgac aaacgcatat gactgctgat aattacgcag
    11701 cgtttcttcg gctatttgca attgcgcata cgccagttgc tgattgaaat agctttgcgc
    11761 gacattagaa accagcagaa tatgcaccgc gcgctgagct tcctcagtgg ctaaataatt
    11821 ttgtcgctcg gcttcgctca tgttctttaa gcgaccgaaa aaatcgaggt caaagctggc
    11881 gttaaggccg gtcgaaaact cccgtgtcgt ggcggtattc cctttaagat taccgctcca
    11941 gctgccgctg ccctcgccat tgagctgtgg gtagcggtcg gcatcggtca gacgatattg
    12001 cgcccgtgcc tcctgcactt tcagcgtcgc catgcgcaaa tcccgattat tcaccagcgc
    12061 ctcgctaatc agcgtcttca cctgattatc aacaaaaaag gtgcgccagc ccgcgttctg
    12121 atagttatct gctgcgttaa ccaggccgtt ctggctgagt gagaactgct gcggcacggg
    12181 cattgccgga cgctgataat ccggtgccag tgaacaaccg gttagcgcaa gggccacaca
    12241 aaatggcaga agtttacaag gagacatagg ctcataattt ctggtgattt tatgccgcca
    12301 actttactcg ccaggctctg attttccggt gacagaaaaa tgacaaaatt gtcattttgc
    12361 caataagcga ttgccatctg atcccgctac tctagaattg cccgggcaac atgcggagga
    12421 aatatgaaac tgttgattgt cgaagatgaa aagaaaaccg gagaatactt gaccaaaggg
    12481 ttaaccgaag ccggttttgt ggtcgatttg gccgacaacg ggttgaatgg ctaccatctg
    12541 gcgatgaccg gtgattatga tctgataatc ctcgatatta tgctgccgga cgtgaacggc
    12601 tgggatatcg tgcgcatgtt gcgttccgcc aataaaggga tgccgattct gttgcttacc
    12661 gcgcttggca ccattgaaca tcgcgtcaag gggctggagt tgggggcgga tgactatctg
    12721 gtgaagccgt tcgcttttgc tgaactgctg gcgcgggtgc gcactctcct gcggcgcggg
    12781 gcggcggtga ttatcgaaag tcagtttcag gttgccgatc tgatggtcga tctcgtcagc
    12841 cgcaaagtca cccgcagcgg cacgcgcatc actctgacca gtaaagagtt tactctgctg
    12901 gagtttttcc tccgccatca gggcgaagtg ctgccccgct cgcttatcgc ctcgcaggtg
    12961 tgggacatga atttcgacag cgacactaac gccattgatg tagcggtgaa gcggctgcgc
    13021 ggcaaaatcg acaacgactt tgagccgaag ctaattcaga ccgtgcgcgg cgtgggctac
    13081 atgcttgagg tgccggatgg tcagtaagcc atttcagcgc ccgttttcgc tggcaacccg
    13141 cctgaccttt tttatcagcc tggccaccat cgcggcgttt ttcgcctttg catggatcat
    13201 gatccactca gtaaaagtgc attttgccga gcaggatatt aatgatttaa aagagattag
    13261 cgccacgctt gaacgggtac ttaatcaccc tgacgaaacg caagcccgac gcttaatgac
    13321 gctggaagat atcgtcagtg gttattccaa cgtgttgatt tccctggcag atagccacgg
    13381 caaaacggtg tatcactccc ccggtgcgcc tgatatccgc gagtttgcac gtgatgccat
    13441 acccgataaa gacgcgcggg gtggcgaggt gtatctcctt tccggcccga cgattatgat
    13501 gccaggccac ggtcacgggc atatggaaca cagcaactgg cggatgatta acttgtcggt
    13561 tggcccgttg gtggacggca aaccgattta tacgctctac atcgcgcttt cgatcgattt
    13621 tcatcttcat tacataaatg atttgatgaa taaacttatt atgaccgcat cggtaatcag
    13681 catcctgatc gtctttatcg tactgttggc ggtacataaa ggtcacgcgc cgatccgcag
    13741 cgtcagccgt caaatccaga atatcacctc gaaagatctc gacgttcgcc ttgacccgca
    13801 gaccgtgccc attgagctgg aacagctggt actgtcgttc aaccatatga tcgagcgtat
    13861 tgaggatgtc tttacccgcc agtccaattt ctcagcggat atcgctcacg aaattcgcac
    13921 gccgattacc aatctcatca cgcaaacgga aatcgccctc agccagtctc gcagccagaa
    13981 ggagctggaa gatgtgctct actctaatct cgaagagctg acgcgaatgg cgaaaatggt
    14041 cagcgatatg ctgtttctcg ctcaggccga taacaaccag ctaatccccg aaaagaaaat
    14101 gctcaacctg gcggatgaag tcggcaaagt gttcgatttt ttcgaggcgt tagcggaaga
    14161 tcgcggcgtg gagttgcgat ttgttggcga cgaatgtcag gttgcgggcg atccgctgat
    14221 gctgcgtcgg gcgttaagca acctgctttc taatgccctg cgttatacgc cgcccggaga
    14281 ggcaattgta gtgcgctgcc agacggtcga tcaccaggtg caagttaccg tcgaaaaccc
    14341 cggtacgccc attgcgcccg agcacttacc gcgattgttt gaccgtttct accgcgttga
    14401 cccttcccgc cagcgaaaag gcgaaggtag cggcattggg ctggcgatag tgaaatcgat
    14461 tgttgtcgcg cataaaggca cggttgcagt aacgtcagat gcgcggggga caaggtttgt
    14521 gattatgctg ccggagagag agtgatta
//
LOCUS       DAJOWB010000116        14319 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJOWB010000116 DAJOWB010000000
VERSION     DAJOWB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.116"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            147..1280
                     /gene="ompN"
                     /locus_tag="OHO85_003778"
     CDS             147..1280
                     /gene="ompN"
                     /locus_tag="OHO85_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCQ2673097.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1421..1855
                     /gene="uspF"
                     /locus_tag="OHO85_003779"
     CDS             1421..1855
                     /gene="uspF"
                     /locus_tag="OHO85_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCQ2673098.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            2031..2966
                     /gene="ttcA"
                     /locus_tag="OHO85_003780"
     CDS             2031..2966
                     /gene="ttcA"
                     /locus_tag="OHO85_003780"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCQ2673099.1"
                     /translation="MSQNQEISKKGQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGINHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3095..4468)
                     /gene="dbpA"
                     /locus_tag="OHO85_003781"
     CDS             complement(3095..4468)
                     /gene="dbpA"
                     /locus_tag="OHO85_003781"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCQ2673100.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4946..5929)
                     /gene="zntB"
                     /locus_tag="OHO85_003782"
     CDS             complement(4946..5929)
                     /gene="zntB"
                     /locus_tag="OHO85_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCQ2673101.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHESAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            6184..7416
                     /gene="dgcM"
                     /locus_tag="OHO85_003783"
     CDS             6184..7416
                     /gene="dgcM"
                     /locus_tag="OHO85_003783"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCQ2673102.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7437..8000)
                     /gene="smrA"
                     /locus_tag="OHO85_003784"
     CDS             complement(7437..8000)
                     /gene="smrA"
                     /locus_tag="OHO85_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCQ2673103.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(8330..9238)
                     /locus_tag="OHO85_003785"
     CDS             complement(8330..9238)
                     /locus_tag="OHO85_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2673104.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            9414..10724
                     /gene="abgA"
                     /locus_tag="OHO85_003786"
     CDS             9414..10724
                     /gene="abgA"
                     /locus_tag="OHO85_003786"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HCQ2673105.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            10724..12169
                     /gene="abgB"
                     /locus_tag="OHO85_003787"
     CDS             10724..12169
                     /gene="abgB"
                     /locus_tag="OHO85_003787"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCQ2673106.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            12206..13732
                     /gene="abgT"
                     /locus_tag="OHO85_003788"
     CDS             12206..13732
                     /gene="abgT"
                     /locus_tag="OHO85_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HCQ2673107.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            13743..14258
                     /gene="ogt"
                     /locus_tag="OHO85_003789"
     CDS             13743..14258
                     /gene="ogt"
                     /locus_tag="OHO85_003789"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCQ2673108.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
BASE COUNT         3615 a         3586 c         3509 g         3609 t
ORIGIN      
        1 tatcatttca gaggaattat ttgattaagg ttttacttaa ggcgtaacaa atgatattta
       61 tcggctaact gaacttctcc tttaggatgt tttcactccc cgaaatgggg ataaaaggca
      121 aataaaataa ctaaaggatt tattcaatga aaagcaaagt actggcactt ttaattcctg
      181 ccctgctcgc cgcaggtgct gcacatgcag ccgaagttta taataaagac ggcaacaaat
      241 tagatctgta tggcaaagtt gatggcctgc attatttttc tgataattcg gcgaaagatg
      301 gcgaccagag ctatgcgcgt ctgggtttta aaggcgaaac ccaaattaac gatcaactca
      361 ctggctacgg tcaatgggaa tacaatattc aggcaaacaa cactgaatct tcaaaaaacc
      421 agtcatggac ccgtctggca tttgccgggc tgaaatttgc agattacggt tctttcgatt
      481 acggacgtaa ttatggcgta atgtacgaca tcgaaggctg gaccgatatg ctgcctgaat
      541 ttggcggtga ctcttatacc aatgcagaca actttatgac tggtcgagcc aatggcgtcg
      601 cgacttatcg taatactgat ttcttcggtc tggtaaatgg tctgaacttc gcggtgcagt
      661 atcaaggtaa caacgaaggt gccagtaatg gtcaggaagg caccaacaac ggacgtgatg
      721 ttcgccatga aaacggtgac ggctggggtc tttccacaac atatgattta ggcatgggct
      781 ttagcgctgg tgcggcatac acctcttctg accgcaccaa tgatcaggtt aaccatactg
      841 cggcgggtgg tgataaagca gacgcgtgga ctgctgggct aaaatacgat gctaacaata
      901 tttacctggc aaccatgtat tcagaaacgc gtaatatgac cccgtttggc gacagcgatt
      961 atgctgttgc aaacaaaacc cagaactttg aagtcactgc acagtaccag tttgattttg
     1021 gtctgcgtcc ggcagtctct ttcctgatgt ctaaaggccg tgacctgcac gctgccggtg
     1081 gtgcagacaa cccggcaggt gttgatgata aagatctggt taaatacgcc gatgttggcg
     1141 cgacttacta tttcaataaa aacatgtcca cttatgttga ctataaaatc aacctgttgg
     1201 atgaagatga cagtttctac gctgccaatg gcatctctac cgatgatatt gtcgctttag
     1261 gtctggttta tcagttctaa atcctcctgc ccgctgttat ggcgggcttt ttctgcttat
     1321 tcttcctcct ttgatctaaa ttaaaaatgt gaactccgtc attacacaaa aagtgtcatc
     1381 tggcgttaca ctttatgcgg atactaaaac aggaggtttt atgaacagaa cgattcttgt
     1441 ccctatcgat atttccgatt cagaattaac tcaacgcgtg attagccacg ttgaggcaga
     1501 ggcaaagatt gatgatgcag aggttcattt cctgacggta ataccttcac tgccctacta
     1561 tgcctctctg ggtttagcgt attccgcaga attaccggca atggatgacc tgaaagcgga
     1621 agccaaatcg caactggaag agatcattaa aaaatttaaa ctgccaaccg acagagtgca
     1681 tgtccatgtt gaggaaggct cgcccaaaga ccgcattctg gaattggcga agaagatccc
     1741 cgctcatatg atcatcattg cttcccatcg accggatatc accacttatc tgctcggttc
     1801 caacgccgca gctgtagtgc gtcacgcaga gtgctccgtg ctggttgtgc gctgacacta
     1861 acgcccgcac attgctgcgg gctttttgat tcatttcgca aatgtgctga cattttcccc
     1921 tctaatccgt accatacacg ccacagtttt tatatcagat ttcttatgct gggcgttccg
     1981 gcatgacgca tactcttctg atgccatata acgaattgag tcgcttttaa atgtcgcaaa
     2041 atcaagaaat tagtaagaaa ggacaataca acctgaacaa attacaaaaa cgtctgcgtc
     2101 gtaacgtggg cgaagccatt gctgacttca atatgattga agaaggcgat cgcatcatgg
     2161 tttgcctctc agggggtaaa gacagctata ccatgctgga gattctgcgc aatttgcagc
     2221 aaagcgcgcc aatcaatttt tcgctggtgg ctgttaacct cgatcaaaag caaccgggct
     2281 tcccagaaca cgttctgccc gagtatcttg aaaagctggg cgttgagtac aagattgtcg
     2341 aagagaacac ttacggtatc gtgaaagaga agattccaga gggcaaaacc acttgctcac
     2401 tgtgttctcg ccttcgtcgc ggtatccttt atcgtaccgc aacggaactg ggggcgacga
     2461 agatcgcgtt gggtcaccat cgtgacgata tcctgcaaac gttgttctta aatatgttct
     2521 acggcggcaa gatgaaaggt atgcctccga aactgatgag cgatgatggc aaacatatcg
     2581 ttattcgtcc gctggcctac tgccgcgaga aagatattca gcgatttgcc gatgcaaaag
     2641 cgttcccgat tattccgtgc aacctgtgcg gttcacagcc taacctgcaa cgtcaggtga
     2701 ttgctgacat gttgcgtgac tgggataaac gttatccagg gcgtatcgag acgatgttca
     2761 gcgcgatgca gaatgtagtg ccgtcgcatc tgtgcgatac caacctgttc gattttaaag
     2821 gcattaacca cggttctgaa gtggttaacg ggggtgatct ggcgtttgat cgcgaagaga
     2881 tcccactaca accggcgggc tggcagccag aagaagatga aaatcagttg gatgagttac
     2941 gactgaatgt ggttgaagtg aaataaccag gatagcgccc gatgcgcaag cgtattgggc
     3001 tactcttatg gaggccggat aagacgcggc cagcgtcgca tccggcaatc ccgaataaga
     3061 tgtttactct tgcacccggc aattcaacat ttcattattt taataaccgc acccggcacg
     3121 tttttccttt aatcttcccg ccctgtaact gtttccatgc tttatgagca acagcctgac
     3181 ggaccgcgac atagacatgc gccggatgca cggcgatttt gccaatatct gcgccatcaa
     3241 gcccgatatc tcctgtcagt gcacctaata catcacccgg gcgcattttg gcttttttcc
     3301 cgccatcgat acacaacgtt gccatttctg cttccagcgg cacaatggaa ctattagctg
     3361 gcggcgtttg ccagttaagt tttatctgca acatgtcaga aatgatattg gcccgctgtg
     3421 cttcttccgg agcacagaaa ctgatcgcca gaccgctatt tcctgcacga gctgtacgac
     3481 cgatgcgatg tacatgaact tcagggtccc acgccagctc aaagttcacc accagctcaa
     3541 gcgatttaat atccagacca cgcgcagcaa catcagtagc aaccagtaca cgggcgctac
     3601 cgttagcaaa acgtaccaga gtctgatcgc gatcgcgttg ctccagatcg ccgtgtaacg
     3661 acaatgcact ttgccctact tcattcagcg cgtcgcagac agcctggcaa tcttttttgg
     3721 tattgcaaaa caccacgcaa gaggatggct gatgcaagct taataaccgt tgcaacagag
     3781 gaattttgcc tttgctggat gtctcataaa attgttgttc aatgggtggc aaagcatctg
     3841 ttgagtcaat ttcaatcgcc aaaggatcgc gttgcactcg tccgctgatt gcggcgatgg
     3901 cttccggcca ggttgccgaa aacagaagcg tctgtcgaga tgcaggcgca aaacggatga
     3961 catcatcaat ggcatcgcta aatcccatat ccagcatgcg gtcggcctca tccatcacca
     4021 aagtattcag cgcatccagt gataccgtgc ctttttgcaa gtgatccagc aaacgacccg
     4081 gcgttgccac gataatatgc ggcgcatgtt gcaacgaatc acgctgcata ccgaacggtt
     4141 gaccaccgca caacgtcaaa attttggtat ttggcagaaa acgcgccagc cgacgcaatt
     4201 cacctgccac ctgatccgcc agttcacgcg taggacacag cactaaagcc tgggtttgaa
     4261 atagcgacgc atcaatttgc tgtaacaagc cgaggccaaa agccgccgtt ttgccgctgc
     4321 cggttttcgc ctgcacgcga acatcttttc cggcaaggat cgccggaagc gcggcggcct
     4381 gcaccggcgt catggttaaa taacccaact cattaaggtt cgtgagttgg gcgggaggca
     4441 aaacattcag ggtagaaaaa gcggtcacaa tctattctcg tggtcatcga cgcaaagtta
     4501 gcaggcgcgt atcctcgcag atctacgctc acgatgcgac aatttaatcg gttcttcatc
     4561 aggtggtggg tcaggcatgg gttgcgggcg agggatcgga tcgggcactg gaacaggatc
     4621 gccaggaatc ggttcaggga caggaatttg caaataaata agtgtcgtca tatttccctc
     4681 cggtcattgg gtggactctt aaagggtaga cgctgataaa taacaggcaa aaaaaagccg
     4741 actcatcaaa gtcggcgtcg tacgaatcaa ttgtgctatg cagtaattca aaaaaggaag
     4801 taagacaata tggagcgcaa cgcccatcgc ttgacgttgc attcacctgc aagggagata
     4861 ttgccctgaa tgggtagaga gtttattgac ttcgctcaaa ctttgcggcg tttttgtata
     4921 cagacagccg gaaaaattgc ttttgttaca accatttact acgatgcaac cataaagcaa
     4981 caccaccaat aagaacaact aacagaatac aaaaaattga aaatccgaat tgccacccgc
     5041 cgccagggat cccaccaagg ttgacgccaa ataaccctgt cagaaaggta ctgggtaaaa
     5101 agaccattgc catcaatgac attgtatagg tacgacgagc taaattttcc tgcatcacct
     5161 gagcgatttc atccgccatc acgccagtcc gtgctataca ggcgtcgatt tcgtcaagac
     5221 cgcgcccaag gcgatcggca atatcctgca tccgacggcg ttggtcatcg ctcatccacg
     5281 gcaaacgttc actggcaaga cgagcataaa catcacgttg cggtgccata tagcgacgca
     5341 tcacgattaa ttgtttgcgc agcagagcca ggaatccacg cggtggaatt tgctgatcaa
     5401 ggagattatc ttcaaggtcg ataattttat cgtgcagctg ctcgataaat tcgctggaat
     5461 gatcggtcaa cgcatcgcac acatccacca gccaccctcc gcaatcggtc ggacccgtgc
     5521 cctcttccag atcgctcacc acatcgtcca gcgccagcac tttgcgttgt cgggtcgaaa
     5581 caattaaccg cccgtccata tatacacgca tggcgaccag ttgatcgggg cgttcatcgg
     5641 tgctgccgtt tatacagcgc aatgtaatca gcgtgccttc accgagacgg ctgactcggg
     5701 gccgagtgct ctcgcctgcc agcgcatcac gtacgttatt gggaagcagc ggtgttgtcg
     5761 ccagccattg ggcgctttca tggtgtacat aattaaggtg gagccagcag ggatgcgctt
     5821 catcaatcac atctgtattt tccagcggtt taacgccgcc tctaccatcc agcatccagg
     5881 caaatactgc atccgggaca ttaacgtccg atcccttaat cgcttccaca gtgcctccat
     5941 catcaacgca ttattttgta gtctagcctt ctggccctgt tacgcaacat ctcatcaccc
     6001 cattaccctg aaatgattaa taaaattctg tctaaattga atacaaaaag caaaatgctt
     6061 ttccgtatac aaaccgtgtg aagtgttaaa tagcgtctat cattatcaga attatctgat
     6121 catatgacgt ggcttttttg cgatcggata gcaacaaaaa ttgataaaaa taacgggatc
     6181 tcaatgatta cgcacaactt caataccctg gacttactca ccagtcctgt ctggattgtt
     6241 tcgccctttg aggaacagtt aatttatgcc aatagcgcgg cgcgactgtt gatgcaagac
     6301 ctcacgttta gtcagctacg aaccggaccc tattccgtct cctcacaaaa agaactgccg
     6361 aaatacctct ccgatctgca aaaccaacac gatattatcg aaatcctcac tgttcagcgt
     6421 aaagaagagg aaacagcatt aagctgtcgg cttgttttgc gagagctgac agaaacagaa
     6481 ccggtgatta ttttcgaagg tatcgaagcg ccggcaacgc tgggtttaaa agccagtcgc
     6541 tcggcaaatt atcagcgcaa aaaacaaggt ttttatgcgc gcttttttct gactaactct
     6601 gcaccaatgt tgttgattga cccctcacga gatggccaaa tcgtcgatgc taacctcgcc
     6661 gcgctcaatt tctatggtta taaccatgaa acgatgtgcc agaaacatac ctgggaaatt
     6721 aatatgctcg ggcgtcgcgt catgcctatc atgcatgaaa tttcgcattt acccggcggt
     6781 cataaacctt tgaattttgt tcataaactg gcggatggat ctactcgtca tgtgcagacc
     6841 tatgccggac cgattgaaat ttatggcgac aagctcatgt tatgtattgt gcatgatatt
     6901 actgagcaaa aacggctgga ggagcagctg gaacatgctg ctcaccatga cgcgatgacc
     6961 ggattactga atcggcgaca gttttatcac attaccgagc caggccaaat gcagcatctc
     7021 gccatcgctc aggattacag cttgttgctc atcgacaccg atcgttttaa acacattaac
     7081 gatctctatg ggcattctaa aggtgatgag gtgttatgcg ccctcgcccg cacccttgaa
     7141 agttgcgctc gcaaaggcga tttggtgttt cgttggggag gcgaagagtt tgtcttattg
     7201 ctaccaagaa ccccactgga taccgcgctt tcgctggctg aaactatccg tgtaagcgtg
     7261 gcaaaagtga gtatttcggg cttaccacgc tttaccgtca gcattggtgt ggcgcatcac
     7321 gaaggaaatg aaagcatcga tgaactgttt aaacgcgttg atgatgcttt gtatcgggcg
     7381 aaaaatgatg gacgcaaccg cgtgctggcg gcataagccg cggatgcgtc tcgagatcaa
     7441 cgactgcgct tagcgtggcg ctcccagttt tcttgcttcg cctgcgccgt tttacgtagt
     7501 gcgacgtaac acgccccgct gccgccatga tgcggtagcg cagtgcaata tgcctgaaca
     7561 tcatcaaatt cggtcagcca gcgcgccaca tagctgcgga caatattggc atgcgattta
     7621 tcatcccgtc ctttgccatg aataatcagc acgttacgca aaccatcagc catggcttgt
     7681 tgaatgaaac tgaacatcat tttgcggcac tcttccaccg gctggcgcaa aagattcagg
     7741 ctcgcctgtt gcggatattt accactgcgc agcttatcca gcaccccatg ttgcaaccct
     7801 tcccgccgaa actccagcgg ctgacttagt gggatgatgt cgagaaatcc ggtggtgagg
     7861 aaattatcaa gctgcagcgt gtcgatacgc tgcggcgcac gttggttacg cgttggatgc
     7921 cagtggacat cggtagcacg tttcagcggc tggacatctt ccatggcgtc aagaaacagc
     7981 gatttgtcgt caaggttcat gttacatcct cccgcaatta agagcgcgat atgataacca
     8041 gaccgggtcg gtccaacaac gtattaccca aaattccagt aataagttcc aaatattgcc
     8101 gatattttaa gcaaaatgct tatgcatgat tattcattca cgatattaat aatgtaactt
     8161 atattttcgt gaaatctgtc actgaagaaa attggcaact aaaggttaaa accgttataa
     8221 cacagtcacc ggcgcagagg agacaatgcc ggacttaaga cgcggatgca ctgctgtgtg
     8281 tactgtagag tctggcggat gtcgacagac tctatttttt tatgcagttt taactttgca
     8341 gatagccgca ttctcgccgg aattgcgtga ttaatgacgc ggtcagtggt gtctggcgac
     8401 tatcacgccg ctgaatcaaa taataggccg ctttcggtaa aatttcgcta accggcaaca
     8461 tcaccagccc ctgtccgtgc aagggatcgc agcccatttc ttcaggcagt atgctgagaa
     8521 aatcgctttt tgccaccaga ctgatacagg ctgagaacgt ctcgcagact acaccgacct
     8581 gtggcgtttg cgcctgatcg tcaagcaatt cactcaactg tttgtagtaa ctgccgtgtg
     8641 gcgtcggcat tgtccagctg taatccagta actgtttgat cgaacgggca ccaatggcgg
     8701 ggtgtcccgg gcggcaaaag atcgcgaatt gcttttccag taatttctca aaagtaaatt
     8761 cgtggtcgta cggtccctga taataggtat tgatggtgaa atccaattct ccctgacgca
     8821 attcattaat catcgacacc agttgccctt ccataatgcg tacttttacc tgcggatgct
     8881 gctgatgaaa acgagatatg acagctggca tcagactgcg ggaaatactg gcccccatgc
     8941 cgatattaat ctgccctgcc agttgccctt gtcgttggcg aatttcctct tgcgctgcgc
     9001 gcagctcttc aagaataaga ctggcgtgct gataaaaact ttcaccggca tcagttaacg
     9061 tcacgccttt actacggcga aaaaagagtt gcgccgctaa tccttcttcc agctcctgaa
     9121 tagatttact cagtgccggt tgcgacatat tcaacattcg gctcgctccg cgaatgctgc
     9181 cctgacgagc cacttcaaca aaagcacgaa tttgatgaat ttttacctga aaagccatga
     9241 cgccaccgat aaccgttatt tatcagacca aagaaactgg catctacttt aatgcagatg
     9301 attgtgtcag ggtaatttat gaacggttaa aactgtgaaa aatcagttag tgataagtaa
     9361 aaactatcgc tacgtgaacc gggtcacact ttttactgat gacgggaaag gttatggagt
     9421 ctttgaatca atttgttaat tcgcttgccc caaaattatc gcactggcga cgtgattttc
     9481 atcactatgc agagtctggc tgggtggaat tccgcactgc cacccttgtt gcggaagaat
     9541 tgcagcagct cggctattca ctggcgctgg gccgcgaagt agttaatgaa agtagccgga
     9601 tgggactacc tgatgaactc actctacaac gcgaattcga gcgcgctcgt caacagggtg
     9661 cgctagcaca atggattgcg gtttttgaag gtggtttcac tggtatcgtc gccaccctgg
     9721 ataccggtcg ccccggtccg gtgatggctt tccgtgtcga tatggacgcg ctggatctca
     9781 gtgaagagca ggatgtcagc catcgcccct accgcgacgg ttttgcgtca tgtaacgccg
     9841 gaatgatgca tgcctgtggt catgatggac ataccgccat tgggcttggg ctggcgcata
     9901 cccttaaaca attcgagtcc ggactacatg gcgtcatcaa actgattttt cagcctgcag
     9961 aggaaggtac gcgtggcgcg cgggcgatgg tcgatgcagg tgtcgtagat gatgttgatt
    10021 attttactgc cgtgcacatt ggcactggcg tacctgcggg caccgtggtg tgcggcagtg
    10081 ataattttat ggcaaccacc aaatttgacg cgcacttcac cgggaccgcc gctcacgcag
    10141 gcgcaaaacc agaagacggt cacaatgcct tgttggcggc agcacaagca actcttgcac
    10201 tgcatgcaat cgccccgcac agcgaaggag cttccagagt aaacgtgggc gttatgcagg
    10261 caggaagcgg ccgtaacgtt gttcctgcct cggcgttgct gaaagtggaa acacgcgggg
    10321 ccagcgacgt cattaatcaa tatgtttttg accgtgcaca acaagcgatt cagggcgcag
    10381 caaccatgta tggtgtcggc gttgaaactc gtctgatggg tgcagctacc gccagttctc
    10441 cttcgccgca atgggtcgca tggttgcaaa gtcaggcggc tcaggtcgcg ggggtcaatc
    10501 aggccattga acgtgttgaa gcgcctgcgg gttccgaaga tgccacatta atgatggccc
    10561 gcgtgcagca acatcaaggg caagcctcct acgtggtgtt tggcacacag ctggcggcag
    10621 gtcatcacaa cgaaaaattc gattttgacg agcaggttct cgctattgcc gtcgaaacgc
    10681 tggcgcgcac cgcgctcaat tttccctgga cgcgaggtat ctgatgcagg aaatctatcg
    10741 ttttatcgac gatgcgattg aagccgatcg ccaacgttat accgatattg ccgatcaaat
    10801 ctgggatcat ccagaaacac gttttgaaga gttctggtca gcggagcatc tggcttcggc
    10861 gctggaatct gcaagcttca ccgttacccg caacgtaggc aatatcccaa atgcatttat
    10921 tgcttcgttt ggtcaaggca aaccggttat cgccctgctg ggagaatatg acgccctggc
    10981 aggtttaagt cagcaagctg gttgcgcgca acctacatcc gcgacgcccg gtgaaaatgg
    11041 tcacggttgc ggacacaatt tgctgggaac cgccgccttt gccgctgcaa tagccgtcaa
    11101 gaaatggctg gaacaatatg ggcaaggcgg cacagtgcgc ttttatggtt gtcctggcga
    11161 agaaggcggc tcgggtaaaa cgttcatggt ccgcgagggg gtatttgatg atgtggatgc
    11221 ggcactcacc tggcacccgg aagcctttgc cggcatgttc aatacccgca cgctggcaaa
    11281 cattcaggca tcatggtgct ttaaagggat cgcagcacat gccgcgaatt cccctcattt
    11341 gggacgcagc gcccttgatg ccgtaacgtt gatgaccact ggcaccaact tcctcaacga
    11401 acatattatt gaaaaagcgc gcgtacacta tgccatcaca aatagcggcg ggatctcgcc
    11461 caacgtggtc caggcgcagg cagaagtgct ttatcttatc cgcgcccccg aaatgaccga
    11521 cgtgcagcat atttatgatc gggtcgccaa aatcgccgaa ggtgcggcat tgatgaccga
    11581 aaccacggtt gaatgccgct tcgacaaagc ctgttccagt tatctcccga atcgcacctt
    11641 agaaaatgcc atgtaccatg ccctatccca ttttggtacc ccggaatgga actccgaaga
    11701 actggctttt gcgaaacaaa ttcaggctac gctcaccccc aacgatcggc aaaacagtct
    11761 gaataatatc gccgcaaccg gtggcgaaaa cggcaaggct tttgcactac gtcatcgtga
    11821 aacggtactg gcgaatgagg tcgctccata tgccgccacc gataacgtgc ttgcggcatc
    11881 gactgatgtc ggcgacgtca gttggaaact gcctgttgcc cagtgtttca gcccctgttt
    11941 tgccgtcggt acaccgctac atacgtggca actggttagc caggggcgaa catctattgc
    12001 tcataaagga atgctgctgg cggcgaaaac tatggcagca accacagtca atctcttcat
    12061 tgattcaggg ctattgcaag aatgccaaca agagcatcag caagttacgg acacgcaacc
    12121 gtatcactgc cctatcccga aaaacgtgac accgtcacct ttaaaataac aacaacaacg
    12181 caaacacaac aaccgaggaa tgcccatgag tatgtcatcc ataccgtcgt cctcccaatc
    12241 cgggaagctc tatggctggg tcgaaagaat tggtaacaag gttccccatc cttttttgct
    12301 ctttatctat ttgataatcg tactcatggt gacgacggca attttgtcag cctttggcgt
    12361 cagtgcgaaa aacccgaccg atggtacgcc ggtggtggtg aaaaacctgc tcagtgtaga
    12421 aggattacac tggtttttac ccaatgtgat taaaaacttt agcggttttg ctccacttgg
    12481 agcgatcctg gcgctggttt taggtgccgg cctagcggag cgcgtcggct tactgccagc
    12541 gctaatggtt aaaatggcat cgcatgttaa tgcccgctac gccagttata tggtgctgtt
    12601 tattgctttt ttcagccaca tttcttccga tgcggcgtta gtgatcatgc caccgatggg
    12661 tgcgctgatt tttctggcgg tgggcaggca tccagttgca ggtttactgg ccgccattgc
    12721 aggcgtaggt tgcggcttca cggctaattt actgattgtc acaaccgacg tgttgctgtc
    12781 ggggatcagc acggaagcgg cggctgcgtt caatccgcaa atgcacgtca gtgtaattga
    12841 taactggtat tttatggcca gctccgtagt cgtactgacg attgttggcg gcctgataac
    12901 cgacaaaatc atcgagccac ggttaggtca atggcaggga aacagcgatg agaaactgca
    12961 gacattgacc gaaagtcagc gttttggttt acgcatagca ggtgtcgtat cgctactttt
    13021 tattgctgcg atcgcgctga tggtgatccc ggaaaacggg atattgcgcg atccgattaa
    13081 tcacaccgtg atgccatcac cctttattaa aggtatcgtg ccactgatca ttcttttttt
    13141 ctttgttgtc tcgctggctt atggcatcgc tacccgcaca attcgacgtc aggcggattt
    13201 accgcattta atgattgaac cgatgaaaga gatggcggga tttatcgtga tggtttttcc
    13261 cctagcccag tttgtcgcca tgtttaactg gagcaacatg gggaaattca tcgccgtggg
    13321 gctgaccgat atacttgaaa gttcagggct tagcggcatc ccggcgtttg tcggtctggc
    13381 gttgctttcc tctttcttat gcatgtttat tgccagcggt tccgcaatct ggtcgattct
    13441 ggcccccatt ttcgtaccaa tgtttatgct acttggcttt cacccggcat ttgcgcaaat
    13501 cctctttcgt attgccgact catccgtatt gcctttagcg ccggtatctc cttttgttcc
    13561 actatttctt ggattcctgc aacgctacaa accagacgcg aaactgggta cttactattc
    13621 gttagtcttg ccctatccgc ttatcttttt ggtggtatgg ctgttgatgt tgctggcgtg
    13681 gtatcttgtt ggtctgccga taggtccggg gatttaccca cgtttgtctt aagagagaac
    13741 ggatgctgag attacttgaa gaaaaaattg ccacgccact gggtccactg tgggtgattt
    13801 gcgatgagca atttcgcctg cgggcggttg aatgggaaga gtacagcgaa cgcatggtgc
    13861 agctgctgga catccattat cgcaaagaag gctatgagcg catttctgcc accaacccag
    13921 gcggtttaag cgacaagctt cgtgattatt ttgccggtaa tcttagcatt attgatacgc
    13981 ttcccactgc cacagggggg acgccatttc agcgcgaagt ctggaaaact ctacgcacta
    14041 tcccctgcgg gcaggtaatg cattacggcc aactggctga acaattgggc cgccctggcg
    14101 cggcgcgtgc cgttggtgcg gcaaacggat cgaatcccat cagcatcgtc gtaccttgcc
    14161 atcgggttat tggccgaaac ggcaccatga ccggatatgc aggcggagtt cagcgaaaag
    14221 agtggttatt gcgccacgaa ggttatcttt tgctgtaaac attaaacaat ttgtgccagc
    14281 ttgttcacac ttttatgtaa agataccctt aacaactta
//
LOCUS       DAJOWB010000117        14190 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJOWB010000117 DAJOWB010000000
VERSION     DAJOWB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.117"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1194
                     /gene="gltS"
                     /locus_tag="OHO85_003790"
     CDS             <1..1194
                     /gene="gltS"
                     /locus_tag="OHO85_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCQ2673109.1"
                     /translation="ASYTLLVACIALLIGMFVVKFTPFLQKNHIPEAVVGGFIVAIVL
                     LIIDKTSGYSFTFDASLQSLLMLTFFSSIGLSSDFSRLIKGGKPLVLLTIAVTILIAI
                     QNTVGMSMAVMMNESPFIGLIAGSITLTGGHGNAGAWGPILADKYGVTGAVELAMACA
                     TLGLVLGGLVGGPVARHLLKKVSIPKTTEQERDTIVEAFEQPSVKRKINANNVIETIS
                     MLIICIVVGGYISALFKDTFLQLPTFVWCLFVGIIIRNTLTHVFKHEVFEPTVDVLGS
                     VALSLFLAMALMSLKFGQLASMAGPVLIIIAVQTVVMVLFACFVTFKMMGKDYDAVVI
                     SAGHCGFGMGATPTAIANMQTVTKAFGPSHKAFLVVPMVGAFIVDISNSILIKIFIEI
                     GTYFT"
     gene            1425..1544
                     /locus_tag="OHO85_003791"
                     /pseudo
     CDS             1425..1544
                     /locus_tag="OHO85_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227464.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1560..2768)
                     /locus_tag="OHO85_003792"
     CDS             complement(1560..2768)
                     /locus_tag="OHO85_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCQ2673110.1"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTEL
                     GRNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIR
                     EQKRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVS
                     DAGFKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKR
                     LTKSNPISSQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWILATNLPVEIR
                     TPKQLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWL
                     AGVHAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDSLVAATLLTQNL
                     FTHGYVLGKL"
     gene            complement(2838..3967)
                     /locus_tag="OHO85_003793"
     CDS             complement(join(2838..3721,3721..3967))
                     /locus_tag="OHO85_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611355.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCQ2673111.1"
                     /translation="MSLKHSDEFKRDAVRIALTSGLTRRQVASDLSIGLSTLGKWIAS
                     ISDETKIPTQDTDLLRENERLRKENRILREEREILKKAGNIFRSTKAVRFQFITDYRG
                     SLSRSRICRLMGVTDRGLRAWKRRPPSLRQRRDLILLAHIREQHRLCLGSYGRPRMTE
                     ELKALGLQVGQRRVGRLMRQNNITVVRTRKFKRTTDSHHTFNIAPNLLKQDFSASAPN
                     QKWAGDITYVWTREGWVYLAVILDLYSRRVIGWATGDRLKQDLALRALNMALALRKPP
                     PGCIQHTDRGSQYCAHEYQKLLLKHQLLPSMSGKGNCFDNSAVESFFKSLKAELIWRR
                     HWQTRRDIEIAIFEYINGFYNPRRRHSTLGSDESPNDFGKNH"
     gene            complement(3998..4621)
                     /locus_tag="OHO85_003794"
     CDS             complement(3998..4621)
                     /locus_tag="OHO85_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCQ2673112.1"
                     /translation="MLIGYARVSKADGSQSLDLQHDALRAAGVERDNIYDDLASGGRD
                     DRPGLTACLKSLRDGDVLVVWKLDRLGRSLAHLVNTVKELSDRKIGLRVLTGKGAQID
                     TTTASGRMVFGIFATLAEFERDLIRERTMAGLASARARGRKGGRKFALTKAQVRLAQA
                     AMAQRDTSVSDLCKELGIERVTLYRYVGPKGELRDHGGLASVSFRSF"
     gene            4747..7632
                     /locus_tag="OHO85_003795"
     CDS             4747..7632
                     /locus_tag="OHO85_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HCQ2673113.1"
                     /translation="MPRRVTLTDRQKDALLRLPTSQTDLLKHYTLSDEDLGHIRLRRR
                     AHNRFGFALQLCVLRYPGRVLAPGELIPAEVIEFIGAQLGLGADDLVDYAAREETRHE
                     HLAELRGLYGFRTFSGRGASELKEWLFREAEMAVSNEDIARRFVAECRRTRTVLPATS
                     TIERLCAAALVDAERRIETRIASRLPMSIREQLLALLEETADDRVTRFVWLRQFEPGS
                     NSSSANRLLDRLEYLQRIDLPEDLLAGVPAHRVTRLRRQGERYYADGMRDLPEDRRLA
                     ILAVCVSEWQAMLADAVVETHDRIVGRLYRASERICHAKVADEAGVVRDTLKSFAEIG
                     GALVDAQDDGQPLGDVIASGSGWDGLKTLVAMATRLTATMADDPLNHVLDGYHRFRRY
                     APRMLRLLDLRAAPVALPLLEAVTALRTGLNDAAMTSFLRPSSKWHRHLRAQRAGDAR
                     LWEIAVLFHLRDAFRSGDVWLTRSRRYGDLKHALVPAQSIAEGGRLAVPLRPEEWLAD
                     RQARLDMRLRELGRAARAGTIPGGSIENGVLHIEKLEAAAPTGAEDLVLDLYKQIPPT
                     RITDLLLEVDAATGFTEAFTHLRTGAPCADRIGLMNVILAEGINLGLRKMADATNTHT
                     FWELIRIGRWHVEGEAYDRALAMVVEAQAALPMARFWGMGTSASSDGQFFVATEQGEA
                     MNLVNAKYGNTPGLKAYSHVSDQYAPFATQVIPATASEAPYILDGLLMNDAGRHIREQ
                     FTDTGGFTDHVFAACAILGYRFAPRIRDLPSKRLYAFNPSAAPAHLRALIGGKVNQAM
                     IERNWPDILRIAATIAAGTVAPSQILRKLASYPRQNELATALREVGRVERTLFMIDWI
                     LDAELQRRAQIGLNKGEAHHALKRAISFHRRGEIRDRSAEGQHYRIAGMNLLAAIIIF
                     WNTMKLGEVVANQKRDGKLLSPDLLAHVSPLGWEHINLTGEYRWPKP"
     gene            complement(7790..8605)
                     /locus_tag="OHO85_003796"
     CDS             complement(7790..8605)
                     /locus_tag="OHO85_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCQ2673114.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(8823..9659)
                     /locus_tag="OHO85_003797"
     CDS             complement(8823..9659)
                     /locus_tag="OHO85_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCQ2673115.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(9659..10462)
                     /locus_tag="OHO85_003798"
                     /note="aph(3'')-Ib"
     CDS             complement(9659..10462)
                     /locus_tag="OHO85_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCQ2673116.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            10700..10972
                     /locus_tag="OHO85_003799"
                     /pseudo
     CDS             10700..10972
                     /locus_tag="OHO85_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            11035..11621
                     /locus_tag="OHO85_003800"
                     /pseudo
     CDS             11035..11621
                     /locus_tag="OHO85_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366863.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            11680..14190
                     /gene="papC"
                     /locus_tag="OHO85_003801"
     CDS             11680..14190
                     /gene="papC"
                     /locus_tag="OHO85_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial usher protein PapC"
                     /protein_id="HCQ2673117.1"
                     /translation="MKDRIPFSVNNITCVILLSLFCNAASAVEFNTDVLDAADKKNID
                     FTRFSEAGYVLPGQYLLDVIVNGQSISPASLQISFVEPALSGDKAEKKLPQACLTSDM
                     VRLMGLTAESLDKVVYWHDGQCADFHGLPGVDIRPDTGAGVLRINMPQAWLEYSDATW
                     LPPSRWDDGIPGLMLDYNLNGTVSRNYQGGDSHQFSYNGTVGGNLGPWRLRADYQGSQ
                     EQSRYNGEKTTNRNFTWSRFYLFRAIPRWRANLTLGENNINSDIFRSWSYTGASLESD
                     DRMLPPRLRGYAPQITGIAETNARVVVSQQGRVLYDSMVPAGPFSIQDLDSSVRGRLD
                     VEVIEQNGRKKTFQVDTASVPYLTRPGQVRYKLVSGRSRGYGHETEGPVFATGEASWG
                     LSNQWSLYGGAVLAGDYNALAAGAGWDLGVPGTLSADITQSVARIEGERTFQGKSWRL
                     SYSKRFDNADADITFAGYRFSERNYMTMEQYLNARYRNDYSSREKEMYTVTLNKNVAD
                     WNTSFNLQYSRQTYWDIRKTDYYTVSVNRYFNVFGLQGVAVGLAASRSKYLGRDNDSA
                     YLRISVPLGTGTASYSGSMSNDRYVNMAGYTDTFNDGLDSYSLNAGLNSGGGLTSQRQ
                     INAYYSHRSPLANLSANIASLQKGYTSFGVSASGGATITGKGAALHAGGMSGGTRLLV
                     DTDGVGGVPVDGGQVVTNRWGTGVVTDISSYYRNTTSVDLKRLPDDVEATRSVVESAL
                     TEGAIGYRKFSVLKGKRLFAILRLADGSQPPFGASVTSEKGRELGMVADEGLAWLSGV
                     TPGETLSVNWDGKIQCQVNVPETAISDQQLLLPCTPQK"
BASE COUNT         3396 a         3544 c         3725 g         3525 t
ORIGIN      
        1 gccagttata cattattagt cgcatgtatc gcgctactca taggaatgtt tgtcgtaaaa
       61 tttaccccgt tcctacaaaa aaaccacata ccagaagccg tcgttggtgg ctttattgtt
      121 gcaattgttc tgttaattat tgataaaaca tcaggttatt cgtttacttt tgatgcttca
      181 ttgcaaagtt tattaatgct cacattcttt tcctctatcg ggctaagttc tgacttttct
      241 cgactgatta aaggaggaaa gccgttagtt ctattaacta ttgcagtaac gatcctaatc
      301 gccattcaaa atactgtcgg catgagtatg gctgtcatga tgaatgaaag tccatttatt
      361 ggcttaattg caggttcaat tactctaaca ggtggtcatg gtaatgccgg agcatggggc
      421 cctattctcg ctgataaata tggtgtaaca ggcgccgttg aattagcgat ggcttgtgca
      481 acacttggat tagtgttggg tggtttagtt ggcggccctg ttgcccgtca tcttttgaaa
      541 aaggtctcta ttcctaaaac aaccgagcaa gagcgcgaca ctatcgttga agcttttgag
      601 caaccaagcg tcaaaagaaa aatcaatgca aataacgtta ttgaaaccat ttcaatgctg
      661 attatctgta ttgttgttgg tggctatatc agtgcattgt ttaaagatac ttttctgcaa
      721 ctgcctactt ttgtctggtg tttatttgtc ggtattatta tccgtaatac actgactcat
      781 gtatttaaac acgaagtgtt tgagccgacc gtcgatgtat taggtagcgt tgctttatcg
      841 cttttcttgg caatggcgtt aatgtcatta aaatttggtc aattggcaag catggcaggg
      901 ccagtattaa ttatcattgc tgtacaaact gttgtcatgg tgctatttgc ctgctttgtc
      961 accttcaaaa tgatgggcaa agattatgat gctgtcgtga tcagcgcggg tcactgtggc
     1021 tttggtatgg gagcaacacc aacagcaatt gcgaatatgc aaacagtcac aaaagcattt
     1081 ggaccatcac ataaagcttt ccttgtcgtt cctatggtcg gtgcctttat tgttgatatt
     1141 tcaaacagta tcttaattaa aatatttatt gaaattggta catactttac ctaatagcta
     1201 cacactactc ggcgtacttc tcctaacaat ttattatagg gcagtatgcc gagccgcttt
     1261 cttaccccgt atattcccct agtcaatcta tttagcttgc acgtttacga attaattctc
     1321 gtgacaacta aaccaaaatt tgaattatga cgagtatatc tgattattta tactttattc
     1381 gttagaatag tccgtagaaa taaataatca gtaggctaca tggaatgaaa ttaagacatc
     1441 tcgatatctt ttatgctgta atgacttgtg gttccttaac gcgtgcggct gaagttttac
     1501 atatttctca acccgcggcg agtaaagcac tcaaacatgc tgagcactga gagatcccct
     1561 cataatttcc ccaaaacgta accatgtgtg aatagatttt gagtaagcag ggttgcagcc
     1621 acgagtgagt cttcccttgt tattgtgtag ccagaatgcc gcaaaacttc catgcctaag
     1681 cgaactgttg agagtacgtt tcgatttctg actgtgttag cctggaagtg cttgtcccaa
     1741 ccttgtttct gagcatgaac gcccgcaagc caacatgtta gttgaagcat cagggcgatt
     1801 agcagcatga tatcaaaacg ctctgagctg ctcgttcggc tatggcgtag gcctagtccg
     1861 taggcaggac ttttcaagtc tcggaaggtt tcttcaatct gcattcgctt cgaatagata
     1921 ttaacaagtt gtttgggtgt tcgaatttca acaggtaagt tagttgctag aatccatggc
     1981 tcctttgccg acgctgagta gattttaggt gacgggtggt gacaatgagt ccgtgtcgag
     2041 cgctgatttt ttcggccttt agagcgagat ttatacaata gaatttggct tgagattgga
     2101 ttgcttttag tcagcctctt atagcctaaa gtctttgagt gactagatga catatcatgt
     2161 aagttgctga taggtttcca gttttccgct cctaggtctg catattgtac ttttcctctt
     2221 actcgactta accagtacca acccagcttc tcaacggatt tataccatgg cactttaaag
     2281 ccagcatcac tgacaatgag cggtgtggtg ttactcggta gaatgctcgc aaggtcggct
     2341 agaaattggt catgagcttt ctttgaacat tgctctgaaa gcgggaacgc tttctcataa
     2401 agagtaacag aacgaccgtg tagtgcgact gaagctcgca ataccataag ccgtttttgc
     2461 tcacggatat cagaccagtc aacaagtaca atgggcatcg tattgcccga acagataaag
     2521 ctagcatgcc aacggtatac agcgagtcgc tctttgtgga ggtgacgatt acctaacaat
     2581 cggtcgattc gtttgatgtt atgttttgtt ctcgctttgg ttggcaggtt acggccaagt
     2641 tcggtaagag tgagagtttt acagtcaagt aaggcgtggc aagccaacgt taagctgttg
     2701 agtcgtttta agtgtaattc ggggcagaat tggtaaagag agtcgtgtaa aatatcgagt
     2761 tcgcacatct tgttgtctga ttattgattt ttcgcgaaac catttgatca tatgacaaga
     2821 tgtgtatcca ccttaactta atgattttta ccaaaatcat taggggattc atcagagccg
     2881 agtgttgaat gtcttcggcg tggattataa aagccattta tatattcgaa gattgcaatc
     2941 tcaatatctc gccttgtttg ccagtgtctg cgccaaatca actcagcctt taatgattta
     3001 aagaagcttt ctactgcgga gttatcaaaa caattgcctt tcccgctcat ggacggcagc
     3061 aattgatgtt tgagcagtag cttttgatat tcatgagcgc aatattggct cccacggtct
     3121 gtgtgttgaa tacaacccgg tggtggtttg cgtaaagcca acgccatatt cagtgccctt
     3181 aatgcaagat cctgctttaa tcgatcacct gttgcccagc caatcacgcg acgggaatac
     3241 aggtcaagga taacagcaag atagacccat ccttctctgg tccaaacata agtgatatcg
     3301 cctgcccatt tctggttggg tgcgcttgcg ctaaagtctt gttttaatag gttcggtgca
     3361 atgttgaagg tatgatgact atccgttgtc cgtttgaatt tacgcgttcg aacaactgta
     3421 atgttattct ggcgcatcaa acgtccaacc cgacgctgcc caacctgcag gcccagcgct
     3481 ttcaactctt ctgtcatacg cggcctacca tagctcccca aacacaaccg atgctgctca
     3541 cgtatatgcg ctagaagtat aagatcacga cgctggcgca gtgatggagg acggcgtttc
     3601 catgcgcgta aaccacgatc tgttacgccc atcaaacgac atatgcgtga acgtgagaga
     3661 gagccacggt aatccgtaat aaactgaaat ctcacagctt ttgtactgcg aaaaatattg
     3721 ctgccttttt taatatctcc ctctcctccc gaaggatacg gttctctttg cgtaaacgtt
     3781 cattctcacg cagaagatca gtgtcttggg taggaatttt agtttcatcg gaaattgatg
     3841 cgatccattt cccaagcgtg gaaagcccaa tacttaaatc tgacgcaact tgacggcgtg
     3901 ttaagccact agtgagtgct atgcgaactg catcacgctt aaattcatca ctatgcttta
     3961 atgacatata tggtctcctt gataacaaat aatgctctca aaaagaccgg aacgaaaccg
     4021 aggcaagacc accatggtct ctgagctcgc ctttgggacc gacatatcgg tagagagtga
     4081 cgcgctcgat gccgagttcc ttgcagagat cggaaactga agtatcgcgc tgggccatgg
     4141 cggcttgcgc gagacgcacc tgagctttgg tgagcgcgaa ttttcgtccg cccttgcgac
     4201 cgcgcgctct cgcggaggcg agacccgcca tggtgcgctc tcggatcaga tcccgctcga
     4261 actcggccaa ggtggcgaag attccgaaca ccatgcgacc ggacgcagtc gtggtgtcga
     4321 tctgagcgcc ctttccagtc agaacccgca ggccgatctt gcggtctgac agctccttca
     4381 ccgtgttgac cagatgggca agcgatcgtc cgaggcgatc gagcttccag accaccagca
     4441 catcgccgtc acgcaatgac ttgaggcagg cagtcaagcc agggcgatca tcacgaccgc
     4501 cggaagcaag atcatcatag atattgtccc gttcgacacc tgcggcgcgc aaggcgtcgt
     4561 gctgcaggtc gagagactgc gagccatcgg ctttggagac gcgggcatat ccgatcagca
     4621 tgtatcacaa acgttggttt gaggcggcgc ttcggccacg attgcattga cctctggaaa
     4681 tgtatctcaa ccagcttcat aaacaaagcg tcttgaacgc tatcagattt tgaaaaagga
     4741 acatgtatgc cgcgtcgcgt cactctaacc gatcggcaga aagacgcgct gttgcgcttg
     4801 ccgacttcac agacggattt gctcaagcac tatacgctga gtgatgaaga ccttgggcat
     4861 atcaggctgc gtcggcgcgc tcacaacagg ttcggcttcg ccctgcaatt gtgtgtcctg
     4921 cgctatcccg gccgggtgct ggctccaggc gaactgatcc ctgcagaggt catcgaattt
     4981 atcggagcgc agcttggcct gggtgccgac gatctcgtag actatgctgc ccgcgaggaa
     5041 acacggcacg agcatcttgc cgagttacgg gggctctacg gcttccgcac cttctccgga
     5101 cgtggtgcga gcgagctgaa ggaatggttg ttccgagaag ccgagatggc ggtgtcgaac
     5161 gaggatatcg cccgtcgctt cgtagccgag tgccgacgca cccgcactgt ccttcccgcg
     5221 acatccacga tcgagcggct ttgtgccgcg gctctcgtcg atgccgagcg acgcatcgag
     5281 acgaggatcg ccagtcggct gcctatgtcg atccgagaac agttgctggc attgctcgag
     5341 gagacggctg atgatcgggt gacccgtttt gtgtggctgc gccagttcga gcctggctcg
     5401 aactcttcgt cggccaaccg gctgctcgac cggctcgaat atctgcaacg catcgatctc
     5461 cccgaggatc tgcttgccgg cgttcctgcc catcgggtga ctcgtctgcg caggcagggt
     5521 gaacggtatt atgccgacgg catgcgcgat ctcccggagg acaggcggct tgcgatcttg
     5581 gctgtttgcg tctcggaatg gcaggcgatg ttggccgacg cagtggtcga aacccacgac
     5641 cggatcgtcg gccgtctcta ccgtgcttcg gagcgtattt gccatgcaaa ggtcgcagac
     5701 gaagcggggg tggtgcgtga caccctgaaa tccttcgccg agatcggggg cgccctggtc
     5761 gatgcacagg atgatggcca gccgctgggc gatgtcatcg cgagtgggtc agggtgggac
     5821 ggcttaaaaa cccttgttgc aatggcaacc aggctgaccg ccaccatggc cgacgatccg
     5881 ctcaatcatg tgctcgacgg ttatcaccgc ttccgccgat acgctccacg catgttgcgc
     5941 ctgctcgatc tgcgagctgc gcccgttgca ctgccgcttc tggaagcggt gacggccctt
     6001 cgtaccggtt tgaacgatgc cgcgatgacc agcttcttgc ggcccagctc gaaatggcat
     6061 cgccaccttc gggcccagag ggctggcgac gctcgcctat gggagatcgc ggtgctgttc
     6121 catctgcgcg atgcgttccg ctccggagat gtctggctta ctaggtcccg gcgctatggc
     6181 gatctgaaac acgcactcgt tccggcacaa tccatcgcgg aaggcggtcg tctcgctgtg
     6241 ccattgcggc cggaggaatg gctggcagac cggcaagctc gcctcgacat gcggttgcgc
     6301 gagcttggcc gtgccgctcg cgcaggcacg atcccgggcg ggtcgattga aaacggcgtt
     6361 ctgcatatcg agaaactcga agccgccgcg ccgacaggcg ccgaagatct ggtgctcgat
     6421 ctctacaagc agatcccgcc cacgcgcatc accgatctcc tgctggaggt ggatgcggcg
     6481 accggcttca ccgaagcgtt cacccatctg cgcacaggag caccctgcgc tgaccggatc
     6541 gggctaatga acgttatctt ggcggaaggg atcaacctcg gcttgcgcaa aatggcggat
     6601 gcgacaaaca cccacacctt ctgggaattg atccgcattg gacggtggca tgtcgagggc
     6661 gaagcctatg accgggcgct ggccatggtg gtcgaggcac aggcagcgtt acccatggcc
     6721 cggttctggg gcatgggcac gtcggcttcg agcgacggac agttcttcgt cgctacagag
     6781 caaggtgagg ccatgaacct ggtcaacgcg aaatatggca ataccccggg cctgaaagcc
     6841 tatagccacg tctccgacca atatgcgccg ttcgcaaccc aggtgattcc tgcaacggca
     6901 agcgaagcgc cttacatcct cgatggcctg ctgatgaacg atgctggacg ccatatccgc
     6961 gagcagttca ccgacacggg cggcttcacc gatcacgtct ttgccgcatg tgccattctc
     7021 ggctaccggt tcgctccgcg catccgcgac ctgccatcca aacggctcta cgcgttcaat
     7081 ccgtcggccg ccccggcgca cctgcgagcg ttgatcggcg gaaaggtcaa ccaagccatg
     7141 atcgagcgca attggcccga catcctgcgc atcgccgcca ccattgctgc cgggaccgtc
     7201 gcgccaagcc agattctgcg gaaactcgcc tcctatccgc ggcagaacga gctcgcgaca
     7261 gccctgcggg aagtcggtcg cgtcgagcgc accctgttca tgatcgactg gattctggat
     7321 gccgaactcc aacggcgtgc ccagatcggg ctcaacaaag gcgaagctca tcatgcgctg
     7381 aagcgggcaa tcagcttcca ccgccgcggt gaaatccgcg accgttccgc cgaaggccag
     7441 cattaccgca tcgccggcat gaatctgctc gccgccatca tcatcttctg gaacaccatg
     7501 aagctcggcg aggtcgttgc aaaccagaaa cgcgatggaa agctgctatc gcccgatctc
     7561 ttggcccatg tttcgccgct cggatgggaa cacatcaatc tcaccggaga atatcgctgg
     7621 ccaaagcctt agcgtaggat tccgccccct cccgcaaacg accccataaa gggcagggta
     7681 ccggcgttcg atagtaccgt ccggatactg cccgatatag gcgtcttgag cgatgtcctg
     7741 aagcgcggtg tccggaattt caggtttgtg tctctacaaa gactaactat cagaaaaact
     7801 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
     7861 gaaaaagccg tttctgtaat gaaggagaaa attcaccgag gcagttccat aggatggcaa
     7921 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     7981 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     8041 agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     8101 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     8161 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     8221 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     8281 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     8341 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     8401 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     8461 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     8521 cccatttata cccatataaa tcagcatcca ggttggaatt taatcgcggc ctcgagcaag
     8581 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     8641 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
     8701 acacaacgtg gttcccgggg tcgtttgcgg gagagggcga aatcctacgc taaggctttg
     8761 gccaacgata ttctccggta agattgatgt gttcccaggg gataggagaa gtcgcttgat
     8821 atctagtatg acgtctgtcg cacctgcttg atcgcggccg cgatagctag atcgcgttgc
     8881 tcctcttctc catccgcgtt ccaagctgcg gaaaggcacc cataagcgta cgcctggtcg
     8941 agcaggcgac gcggatcgac gtccagcgca cgagagaatg cgtccgccat ctgtgcaatg
     9001 cgtctaggat cgagacaaag gtcgtctctg tcagccggat cgtagaacat attggcggcg
     9061 ccaaagccca cttcaccgac cagaccgacg ggatctatca ccagccagcc gcgactggag
     9121 aacatgatgt tttcatgatg cagatcgcca tgtagcccac gcagttccga ggcattgctc
     9181 atcatttgat cggctataat cgccgcgtgg acgtagtcag tttgacaacc tgcgttttga
     9241 tcatcgcgcg cccgctgaaa caaagctgca aagcgatccc ggatcgggag aagggcagaa
     9301 ggcaggggtt cctcagatgc ggcatacagc ttcgccatta gttccgctgc aatttcggtc
     9361 gcctggtagt cgccgtgctc ggcaacgatg tgagagagca ttcgctcccc ggcatattcg
     9421 agcaacatca gattgttctc acgaccgagc aaccggactg ctcccctccc attgcgccat
     9481 accagatagt cggccccgcg cagttcatca gcaatgtctt ctataggttt caatcccttg
     9541 acgattgcag gagtcccgtc tggcaatgaa actttccaaa cgaggctgga aaaggtgtcc
     9601 gcaatgagaa caggttgcga aacgtgccaa tgagcaggaa aaacaggcgg catgaacatc
     9661 aaccccaagt cagagggtcc aatcgcagat agaaggcaag gcgttcgcgg tcgggggctt
     9721 cgatccccaa tacattgaat aggacagcga aggcgcgctc tgcttcatct ggcgctgccc
     9781 agttctcttc ggcgttagca atcatgagtg ccaaatcggc atagcgatct gctgttccga
     9841 gccgcccaag gtcgatcaga cccgtgcatt gaagagtttt agggtccacc atgaagttcg
     9901 gcatgcaggg atcaccatgg caaacaacca tatcggtgcg ctcttggtcg agccgcaccg
     9961 gtagctctcg ttcgacacga gccaaaagat cgagctgcgg cgtactcttg tcctcgtccg
    10021 gtaagaagtc gggattgacg gcattgcggg acaccacatc aacggcgcgt ccgaacattc
    10081 gcgacagcct gcgctcaaac ggacattgat caaccgatag gctgtgaaca gcgccaagtt
    10141 gctgccccat tgacggccac gctttgagca aatccgctcc agacagatca gccgccggta
    10201 ctcccggaat tgccgttatc accaagcatg caccctcctg ttcctcctgc cagttgatga
    10261 cctcggggca agccacacct cgacctttga gccaaatgag gcggtcacgc tctccagcga
    10321 gctcaccgcg gcgggaagca ggtgcgattt tcgcgaaggc atgcccgtca ccacgtcgaa
    10381 aaacaaaatc accagattct ccgcctctga caggcaacca gtcagaatgc gattcaccaa
    10441 aaaaaatatt agttcgattc aatggaggtt ccttcagttt tctgatgaag cgcggaggtg
    10501 gctcaacctg cgaaaagaaa cgagttgcta cgtaagtccg agaacatgct ttccatggtc
    10561 ttgcaccggt tgtgttccgg tctatctctc attttaagcg gcttttttct caaatgccac
    10621 cggcgatttc cagccgagtg ctgatgaatc ccctaatgat ttttaccaaa atcattaggg
    10681 gattcatcag ggtcaagcag ctaaaaacgg taaggttcaa ttatctttta ctgggccaca
    10741 agtaacgggg caggctgaag aattagcaac taacggcggt acgggcacag ctattgtagt
    10801 tcaggctgca ggcaaaaacg tttctttcga tgggactgca ggtgacgctt atcccctgaa
    10861 agatggtgat aatgttcttc attatacagc tcttgtgaaa aaagcgaatg gcggtactgt
    10921 ttctgaaggt gctttttctg cagttgcaac ctttaattta agttaccagt aattcatctg
    10981 atttttacag ggtgaggcaa gacccaccct gtgtctgaca acgacattta aggtatgaag
    11041 aatttttgtg ttctgtttgt gatgtatttc tggagtacat tacttcatgc tgtatcttca
    11101 gagccatttc ctccgcccgg catgaccctt cctgaatact ggggagaaga acacgtatgg
    11161 tgggacggca gggctgcttt tcatggtgag gttgtcagac ctgcctgtac tctggcgatg
    11221 gaagacgcct ggcagattat cgatatgggg gaaaccccgg ttcgggattt acagaatggt
    11281 ttttccggac ctgaaagaaa attcagcctc cggctcagga actgtgaatt taacagtcag
    11341 ggtgggaacc ttttctctga ttcccggata agggtgactt tcgatggctc cggggtgaaa
    11401 cgccggataa gtttaattta tccggtcagg caaaaggaat taatctgcag atagctgatg
    11461 cctgggggaa tattgcccgg gcagggaaag taatgcctgc aataccattg acgggtaatg
    11521 aagaagcgct ggattacacc ctcagaattg tgagaaacgg aaaaaaactt gaagccggaa
    11581 attattttgc cgtgctggga ttccgggtcg attatgagtg agtcactccg gtgagatgtc
    11641 cggttattta tcttttttgt gaatctggtg atgcgtggaa tgaaagacag aatacctttt
    11701 tcagtcaaca atattacctg tgtgatattg ttgtctctgt tttgtaacgc agccagtgcc
    11761 gttgagttta atacagatgt acttgacgca gcggacaaga aaaatattga cttcacccgt
    11821 ttttcagaag ccggctatgt tctgccgggg caatatcttc tggatgtgat tgttaacggg
    11881 caaagtattt ctcccgcatc gttacagatt tcatttgttg aacctgcgtt gtcaggagat
    11941 aaggcagaaa aaaaattgcc gcaggcctgt ctgacatcag atatggtcag actgatgggg
    12001 ttaacagcag aatctctgga taaagttgtt tactggcatg atggtcagtg tgcggatttt
    12061 catgggttgc cgggagtgga tattcgtcct gataccggag cgggcgtatt acgcatcaat
    12121 atgccgcagg cctggcttga gtattctgat gccacctggc tgcctccctc acgctgggac
    12181 gacggcattc ccggactgat gctggattat aacctcaacg ggacggtttc ccgtaattat
    12241 cagggaggag actctcatca gttcagttat aacgggactg tgggggggaa tctggggccc
    12301 tggcgcctgc gggctgacta tcagggaagc caggagcaga gccgctacaa tggggaaaaa
    12361 acgacaaaca gaaatttcac atggagtcgc ttttatctgt tccgtgccat tccacgatgg
    12421 cgggcaaacc tgacgctggg cgagaataat atcaactcag atatattccg gtcatggagt
    12481 tatacgggag ccagcctgga aagcgatgac cggatgctgc cgcccagact gcgaggctat
    12541 gcaccgcaga ttaccgggat tgcggagact aatgcccgtg ttgtggtgtc gcagcaggga
    12601 cgggtgctgt acgactcgat ggtccccgca gggccattca gtattcagga cctggacagt
    12661 tcagttcgcg gacgtcttga tgttgaggtt attgaacaga atggacggaa gaaaaccttt
    12721 caggtcgata cagcctcggt tccttatctg acgcgtccgg gacaggtccg gtataaactt
    12781 gtctccggtc gttcccgtgg atacgggcat gagaccgaag ggcctgtatt tgcgaccgga
    12841 gaggcatcct gggggctcag taaccagtgg tcgctgtatg gcggggctgt gcttgccggt
    12901 gattataatg cactggcagc cggtgccggc tgggacctgg gtgtgccggg gaccctttcc
    12961 gctgatatca cgcagtcagt agcccgtatt gagggagaga gaacgtttca gggaaaatcc
    13021 tggcgtctga gctactccaa acggtttgat aatgcggatg ccgacattac gttcgccggg
    13081 tatcgtttct cagagcgaaa ctatatgacc atggagcagt acctgaacgc ccgctaccgt
    13141 aatgattaca gcagtcggga aaaagagatg tataccgtta cgctgaataa aaacgtggcg
    13201 gactggaaca cctcttttaa cctgcagtac agccgtcaga catactggga catacggaaa
    13261 acggactatt atacggtgag cgtcaaccgc tactttaatg ttttcggact gcagggtgtg
    13321 gcggttggat tggcagcctc aaggtctaaa tatctggggc gtgataacga ttctgcttac
    13381 ctgcgtatat ccgtgccgct ggggacgggg acagcgagct acagtggcag tatgagtaat
    13441 gaccgttatg tgaatatggc cggctacact gacacgttca atgacggtct ggacagctac
    13501 agcctgaacg ccggccttaa cagtggcggt ggactgacat cgcaacgtca gattaatgcc
    13561 tattacagtc atcgtagtcc gctggcaaat ttgtccgcga atattgcatc cctgcagaaa
    13621 ggatatacgt ctttcggcgt cagtgcttcc ggtggggcaa caattaccgg aaaaggtgcg
    13681 gcgttacatg cagggggaat gtccggtgga acacgtcttc ttgttgacac ggatggtgtg
    13741 ggaggtgtac cggttgatgg cgggcaggtg gtgacaaatc gctggggaac gggcgtggtg
    13801 actgacatca gcagttatta ccggaataca acctctgttg acctgaagcg cttaccggat
    13861 gatgtggaag caacccgttc tgttgtggaa tcggcgctga cagaaggtgc cattggttac
    13921 cggaaattca gcgtgcttaa agggaaacgt ctgtttgcaa tactgcgtct tgctgatggc
    13981 tctcagcccc cgtttggtgc cagtgtaacc agtgaaaaag gccgggaact gggcatggtg
    14041 gccgacgaag gccttgcctg gctgagtggc gtgacgccgg gggaaaccct gtcggtaaac
    14101 tgggatggaa aaatacagtg tcaggtaaat gtaccggaga cagcaatatc tgaccagcag
    14161 ttattgcttc cctgtacgcc tcagaaataa
//
LOCUS       DAJOWB010000118        14169 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJOWB010000118 DAJOWB010000000
VERSION     DAJOWB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.118"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..750
                     /gene="gspC"
                     /locus_tag="OHO85_003802"
     CDS             1..750
                     /gene="gspC"
                     /locus_tag="OHO85_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HCQ2673118.1"
                     /translation="MIFISIGSIIFNVNYFHTTIVKNGQIINQPTNAFQSDFSLAALW
                     RNENHAGVKDANPVAVNQETPKLSIALNGIVLTSNDETSFVLINEGSEQKRYSLNEAL
                     ESAPGTFIRKINKTSVVFETHGHYEKVTLHPGLPDIIKQPDSESQNVLADYIIATPIR
                     DGEQIYGLRLNPRKGLNAFTTSLLQPGDIALRINNLSLTHPDEVSQALSLLLTQQSAQ
                     FTIRRNGVPRLINVSVGELTGMNGLRHERTQ"
     gene            734..2686
                     /gene="gspD"
                     /locus_tag="OHO85_003803"
     CDS             734..2686
                     /gene="gspD"
                     /locus_tag="OHO85_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HCQ2673119.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNARKPSSSGAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPIFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYHSLSKEKYTRYRQEQQQRIDGKSK
                     ALVGSEDLPVLDENTFNSHAPAPSSR"
     gene            2696..4177
                     /gene="gspE"
                     /locus_tag="OHO85_003804"
     CDS             2696..4177
                     /gene="gspE"
                     /locus_tag="OHO85_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HCQ2673120.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQKVISGDTSWDEVMRVASATLESEA"
     gene            4174..5370
                     /gene="gspF"
                     /locus_tag="OHO85_003805"
     CDS             4174..5370
                     /gene="gspF"
                     /locus_tag="OHO85_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HCQ2673121.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNKRLGDVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIGVVIILLTAVVPKITEQFMHMKQQLPLSTRILLGLSDTLQRTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5380..5817
                     /gene="gspG"
                     /locus_tag="OHO85_003806"
     CDS             5380..5817
                     /gene="gspG"
                     /locus_tag="OHO85_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HCQ2673122.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     VVSDIVALENALDMYKLDNHHYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5825..6334
                     /gene="gspH"
                     /locus_tag="OHO85_003807"
     CDS             5825..6334
                     /gene="gspH"
                     /locus_tag="OHO85_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HCQ2673123.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDVTNTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESQND
                     WDEELSIHLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVCSGSWPLD
                     QTLARDTRP"
     gene            6331..6708
                     /gene="gspI"
                     /locus_tag="OHO85_003808"
     CDS             6331..6708
                     /gene="gspI"
                     /locus_tag="OHO85_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HCQ2673124.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRNAIERMRNET
                     LALWIADNQLQSQDSFGEENTSSSGKELINGEEWNWRSDIHSSKDGTLLERTITVTLP
                     SGQTTSLTRYQSIDNKSGQAQDD"
     gene            6701..7288
                     /gene="gspJ"
                     /locus_tag="OHO85_003809"
     CDS             6701..7288
                     /gene="gspJ"
                     /locus_tag="OHO85_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HCQ2673125.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     QTQQYGGLQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7281..8264
                     /gene="gspK"
                     /locus_tag="OHO85_003810"
     CDS             7281..8264
                     /gene="gspK"
                     /locus_tag="OHO85_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HCQ2673126.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTLSFHSQLQRTRQVNHH
                     LQRQYDIELAEKLALASLTQDVKDNDRQTTLQQYWAQPQQLQLEDGNTVKWQLRDAQH
                     CFNLNALAKISDDPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDVDDSPRF
                     HGAEDSFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTTELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPREGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKRHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8279..9442
                     /gene="gspL"
                     /locus_tag="OHO85_003811"
     CDS             8279..9442
                     /gene="gspL"
                     /locus_tag="OHO85_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HCQ2673127.1"
                     /translation="MPESLMVIRSSSTLRKHWEWMTFSADSVSSVHTLTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDNTQDWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQKSWLIR
                     SAAHAFNELDEHWLQHLAAQFPPENMLCYGVVPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGIFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRSIALWHTLKIEDQL
                     QQQQQETWQRYFPQIKRTHNFHFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQKQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGK"
     gene            9439..9901
                     /gene="gspM"
                     /locus_tag="OHO85_003812"
                     /pseudo
     CDS             9439..9901
                     /gene="gspM"
                     /locus_tag="OHO85_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
     gene            9901..10578
                     /gene="gspO"
                     /locus_tag="OHO85_003813"
     CDS             9901..10578
                     /gene="gspO"
                     /locus_tag="OHO85_003813"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HCQ2673128.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHF
                     RQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFVLTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(10607..11083)
                     /gene="bfr"
                     /locus_tag="OHO85_003814"
     CDS             complement(10607..11083)
                     /gene="bfr"
                     /locus_tag="OHO85_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCQ2673129.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(11155..11349)
                     /gene="bfd"
                     /locus_tag="OHO85_003815"
     CDS             complement(11155..11349)
                     /gene="bfd"
                     /locus_tag="OHO85_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCQ2673130.1"
                     /translation="MYVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVR
                     AAREVMEDELMQLPEFKESA"
     gene            complement(11518..14166)
                     /gene="chiA"
                     /locus_tag="OHO85_003816"
     CDS             complement(11518..14166)
                     /gene="chiA"
                     /locus_tag="OHO85_003816"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011703682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HCQ2673131.1"
                     /translation="MCSALPALAMEAWNNQQGGNKYQVIFDGKIYENAWWVSSTNCPG
                     KAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSNTPASNTPANGGSATP
                     AQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNCPGDAKSNDASNPWRY
                     VRAATATEISETSNPQSCTSAPQPSPDVKPAPDVKPAPDVQPAPADKSNDNYAVVAWK
                     GQEGSSTWYVIYNGGIYKNAWWVGAANCPGDAKENDASNPWRYVRAATATEISQYGNP
                     GSCSVKPDNNGGAVTPVDPTPETPVTPTPDNNEPSTPADSGNDYSLQTWSGQEGSEIY
                     HVIFNGNVYKNAWWVGSKDCPRGTSAENSNNPWRLVRTATAAELSQYGNPTTCEIDNG
                     GVIVADGFQASKAYSADSIVDYNDAHYKTSVDQDAWGFVPGGDNPWKKYEPAKAWSAS
                     TVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNSRPWKPLGKAQSYSNEELN
                     NAPQFNPETLYASDTLIRFNGVNYISQSKVQKVSPSDSNPWRVFVDWTGTKERVGTPK
                     KAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVVSKDANTCLPTWGTAYGMQ
                     NYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDLMQHYYDIVDNLNLKVLDF
                     DIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTLPILPTGLTPEGMNVLSDA
                     KAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSAIANLHSQLKGLHPNKSDA
                     EIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLGMVGIWSIARDLPGGTNLS
                     PEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT         3643 a         3645 c         3384 g         3497 t
ORIGIN      
        1 attatattta taagcatagg gtcgattatt tttaatgtga attatttcca tacaactata
       61 gttaaaaatg gacagatcat taatcaacca acaaacgcat ttcagtctga tttcagcctt
      121 gccgctttat ggcgtaacga aaatcatgct ggggtaaaag atgcaaatcc cgtggcagtt
      181 aatcaggaaa cacctaagct ctctatagcg cttaatggca ttgtactcac atcgaatgat
      241 gagacttctt ttgtcttaat taatgagggg agcgaacaaa agcgatactc cctcaacgaa
      301 gcccttgagt cagcgccggg aacgtttatt agaaaaataa ataaaaccag cgttgtgttt
      361 gaaactcacg ggcattacga aaaagtaact ctccatccag ggctacctga catcatcaag
      421 cagcctgatt cagaaagtca aaatgtactc gctgattata ttatcgccac gcctatacgt
      481 gatggagaac agatatacgg gcttcgattg aaccctcgta agggcctcaa tgcatttacc
      541 accagcttgc tacaaccagg ggatatcgcc ctcaggatca ataatctttc gctgacccac
      601 cctgatgagg tttcacaggc attaagttta ctgctaactc aacaaagtgc tcagtttaca
      661 attcgtcgca acggcgtacc ccgcttgata aatgtttccg tcggggaact tacaggaatg
      721 aatggactgc gtcatgaaag gactcaataa aatcacctgc tgcttgctgg cagcactact
      781 catgccttgt gcaggacacg ctgagaacga acaatacggc gcgaacttca ataacgccga
      841 tatccgccag ttcgtggaaa tagtgggtca gcatcttggc aaaacgatcc tgatcgaccc
      901 ttcggtacag ggaaccattt ccgtacgcag taatgatacg tttagccaac aggagtacta
      961 ccagttcttt ttaagtattc ttgatcttta cggttattcc gtgatcacgc tggacaatgg
     1021 ttttctgaaa gtggttcgct cagctaatgt aaaaacatcg ccagggatga ttgctgacag
     1081 ttctcgtcca ggcgtaggtg atgagttggt cacccgaatt gtaccgcttg agaacgttcc
     1141 tgctcgtgac ctggcccccc tgctccgcca gatgatggat gcgggtagcg tcggtaatgt
     1201 tgtgcattat gaaccctcca acgttcttat tctgaccggt cgtgcctcca ccattaataa
     1261 actgattgaa gtcataaagc gcgttgatgt catcggcaca gagaagcagc aaattattca
     1321 tctggaatat gcgtcagcgg aagatctcgc cgagattctt aatcaattaa tcagcgaaag
     1381 ccacggtaaa agccagatgc cagccctcct ctccgcgaag attgtggcgg ataagcgaac
     1441 caactctctt atcatcagtg gaccggaaaa agcacgccag cgcatcactt cattactgaa
     1501 aagccttgat gtcgaagaga gcgaggaagg aaatacccgg gtttattacc tgaaatatgc
     1561 taaagccacg aatctggtgg aagtgctaac cggtgtttcc gaaaagctga aagatgaaaa
     1621 agggaatgcg cgtaagccct cctcttctgg cgcgatggat aacgtcgcca ttaccgccga
     1681 tgaacagact aactctctgg tcattaccgc tgaccagtcc gtccaggaaa aactcgccac
     1741 ggtaattgcg cgtctggaca ttcgccgtgc acaggtgctg gttgaggcaa tcatcgttga
     1801 agttcaggat ggaaatggac taaacctcgg cgtgcaatgg gcgaataaaa acgttggcgc
     1861 acagcaattt accaataccg gattaccgat ttttaacgct gcgcaaggtg tggctgatta
     1921 taaaaagaat ggtgggatca ccagcgcgaa tcctgcctgg gatatgttta gcgcctacaa
     1981 tggcatggcc gcaggcttct tcaatggcga ctggggagta ctgcttaccg cgctggccag
     2041 taacaataaa aatgacatcc tcgccacccc aagcatcgta acgctggata ataaactcgc
     2101 gtccttcaac gtggggcagg atgtgccggt gctatccggg tcacagacca cttcagggga
     2161 taacgtcttt aataccgtcg aacgcaaaac ggtggggaca aaactcaaag ttactccgca
     2221 ggtcaatgaa ggcgacgcgg tgttgctcga aatagagcag gaagtctcca gcgttgactc
     2281 ttcctctaac tcgacgctcg gcccgacgtt taatacccgt actattcaaa acgccgtgct
     2341 ggtcaaaacc ggtgaaacgg tggtcctggg cggattgctg gatgattttt ctaaagagca
     2401 agtgtcaaag gttcctctgc ttggcgatat tcctttagtg gggcaactct tccgctatac
     2461 ctccaccgag cgcgctaaac gcaacctgat ggtatttatc cgtccgacga ttatccgtga
     2521 cgatgatgtt tatcactcac tgtcaaaaga gaaatacacc cgttaccgtc aggagcaaca
     2581 acagcggatc gacgggaaat caaaagcgct ggttggctcg gaagatttgc cggtgctgga
     2641 tgaaaacacg ttcaacagtc acgcccctgc gccatcgtca cggtgaggca ttcatatgag
     2701 aattcactca ccgtaccccg ccagttgggc gctggcacaa cgaattggtt atctctattc
     2761 agagggcgag attatttatc tcgccgatac gccattcgag cggttactcg atattcaacg
     2821 tcaggttggc cagtgccaga ccatgaccag cttgtcacag gctgattttg aagctcggct
     2881 ggaagcggta ttccatcaga ataccggtga gtcgcaacag attgcgcagg atatcgatca
     2941 atccgtcgat cttctctcgc tttcggaaga gatgcccgca aatgaagatc tcctgaatga
     3001 agattcagcg gcaccggtta tccgcttgat caatgcgatt ttgagtgagg ccatcaaaga
     3061 aaccgcctct gatatccaca ttgaaaccta tgaaaaaaca atgtcgatcc gttttcgcat
     3121 cgacggcgtt ttgcggacaa ttttacagcc aaacaaaaaa ctggcggcac tgcttatctc
     3181 ccgaattaag gtcatggctc gtcttgatat cgccgaaaaa cgtattccac aggatggaag
     3241 aattagtttg cgtatcgggc gacgtaacat agatgtccgc gtatccacac tgccgtccat
     3301 ctatggtgaa cgcgccgtac tccgcctgct ggataaaaac agcctccagc tttcattgaa
     3361 caacctgggg atgacggcag cggataagca ggatttagaa aatctcattc agcttccgca
     3421 cggtattatc ctggtgacag ggccgacagg ctccggtaaa agcaccacgc tctacgccat
     3481 cctttcggcg ctgaatactc ccggccgcaa tattctgacg gtagaagatc ccgtggaata
     3541 tgagctggaa ggcattgggc aaacgcaggt gaatacccgt gtggatatgt ctttcgctcg
     3601 cggcctgcgc gccatacttc gccaggaccc ggatgtcgtc atggtggggg aaattcgtga
     3661 tacagaaacc gcgcagattg cggttcaggc ctcgctcacc ggccatctgg tactctcaac
     3721 actccacact aacagtgcat caggcgcagt gacccggctc cgcgacatgg gcgtcgaatc
     3781 attcctgctt tcgtcttccc tggcagggat tatcgcgcaa cgtctggttc gtcgcctgtg
     3841 tccgcaatgc cgacaattca cgcccgtatc accccaacaa gcgcagatgt ttaaatatca
     3901 tcagctcgcg gtgacaacaa ttggcactcc cgtaggctgc cctcattgcc atcaatccgg
     3961 ctatcagggg cgcatggcga tccacgaaat gatggtggtg acgccggaat tacgggccgc
     4021 tattcatgaa aatgtggatg aacaagcact ggagcgacta gtccggcaac aacacaatgc
     4081 cttaatcaaa aatggcctgc aaaaagtgat aagcggtgac acctcctggg atgaggttat
     4141 gcgcgtcgcc agtgccacgc tggagagcga agcatgaatt atcgctatcg cgccatgacc
     4201 caggatggtc aaaaattgca agggatcatt gatgctaacg atgaacgtca ggcacgactg
     4261 cggctgcgtg aagaagggct tttcctgctg gatattcgcc cccaaaaaag ttcgggagta
     4321 aaaacacgtc gcccgaggat cagccatagt gaactgacgc ttttcacccg gcagttggca
     4381 accttaagcg cagcggcatt acccctggaa gagagccttg ccgtaatcgg tcaacaaagc
     4441 agtaataaac gactgggtga cgtgttaaat caggtacgca gcgccatcct tgaagggcat
     4501 cccctttccg atgcattaca gcattttccc acgcttttcg attcgctcta tcgtaccctg
     4561 gtaaaagcgg gcgaaaagag cgggctgctg gccccggtgt tggaaaagct ggctgattac
     4621 aatgaaaacc ggcagaaaat ccgcagcaag ctcattcagt cactgatcta cccctgtatg
     4681 ctcactacgg tggcgattgg ggtcgtgatt attctcctca ctgctgtcgt gcccaaaatt
     4741 accgaacagt tcatgcatat gaagcagcaa ctgccgctga gtacacgcat tcttttaggt
     4801 ctgagcgaca cgttgcaacg taccggcccg acattattag cgacagtgtt tattgtcgct
     4861 gtaggtttct ggctctggtt aaaacgcggc aataaccgcc accgttttca tgccatgttg
     4921 ctgcgcgttg cgctcatcgg cccgctgatt tgcgccatta acagcgcacg ctatctccgc
     4981 actttaagta ttttgcaatc cagcggcgtc cctctgctgg atgggatgaa tttgtccacc
     5041 gaaagcctca acaaccttga aattcgccag cgtctggcaa atgcggcaga gaacgttcgc
     5101 cagggtaaca gcattcatct ttcgctggaa caaaccgcaa ttttcccgcc gatgatgctc
     5161 tacatggtgg cctctggcga aaaaagcggg cagctcggca cattaatggt cagagccgca
     5221 gataaccagg agacactcca acaaaatcgg atcgccttaa cgctctccat cttcgagcca
     5281 gcactcatta ttacgatggc actgatcgtc ctgtttattg tcgtgtcggt actccaacct
     5341 cttcttcaac ttaactcaat gattaattaa ggaaagcata tgcgcgcaac ggataagcaa
     5401 cgcggtttta cattactgga aattatggtg gtgatcgtca ttatcggcgt acttgccagc
     5461 ctggtggttc ctaacctaat gggcaataaa gaaaaggcgg ataagcaaaa agtcgtcagc
     5521 gatattgtgg cgctggaaaa cgcccttgat atgtacaaac tcgacaatca tcactatcca
     5581 accacaaatc aggggcttga atccttagta gaagcgccga cactgccacc gctggccgca
     5641 aactataaca aggaaggtta tatcaagcgt ctgcctgccg atccctgggg caatgattat
     5701 gtcctcgtta atcctggtga acatggtgca tacgatctgc tttcagcagg gcccgatggt
     5761 gaaatgggaa ccgaggacga catcaccaac tggggtttga gcaagaagaa aaagtaagtc
     5821 agcgatgaat cagcaacgcg ggtttaccct gctggagatg atgctggtgc tggcgctggt
     5881 cgctatcacg gcaagcgtgg tgctcttcac atatggccgg gaggatgtca ccaacactcg
     5941 tgcgcgcgaa acagcggcgc ggtttaccgc tgcacttgaa ttagccatcg accgcgccac
     6001 gctcagtggt cagcctgtcg gtatccattt ctctgattca gcatggcgca ttatggtacc
     6061 gggcaaaaca ccatcagcct ggcgctgggt tccattacaa gaagatgccg cagacgagag
     6121 ccaaaatgac tgggatgaag aactctcaat tcatcttcag ccatttaaac ctgatgacag
     6181 caaccagcca caggttgtga ttctggccga cggacaaatc acgcccttct ctttgttgat
     6241 ggctaacgcc gggaccggtg agccgctcct gacgttagtc tgttcaggct catggcccct
     6301 ggatcaaacg ctcgccagag atacccgccc atgaacaaac aatcagggat gacactgctt
     6361 gaagtcttac tggcgatgag tattttcacc gctgttgctc tgactttaat gagcagtatg
     6421 caagggcaac gaaatgccat cgagcgaatg cgtaacgaga cgctggcatt gtggatagcg
     6481 gataaccagc ttcagtcaca agattcattc ggcgaggaaa acacgtccag ttctggcaaa
     6541 gagttaataa atggcgaaga gtggaactgg cgcagcgata tccactcaag caaagacggc
     6601 acgcttctgg aacgcaccat aacggtgaca ttacccagcg gtcagacaac ctcgcttacg
     6661 cgttatcaaa gcatcgataa taaatccggg caggcacaag atgattaatc gccagcaggg
     6721 tttcacattg ctggaggtca tggcggcgct ggcgattttc tcaatgctta gcgtgctggc
     6781 gtttatgatt ttttcgcaag cttccgagtt gcatcaacgc tcacagaaag aaatccagca
     6841 gttcaatcag ctacagcgca ccatcaccat tcttgataac gatctcctgc aactggtcgc
     6901 ccggaggaat cggagtacgg acaaaatcat ggtgttgggt gaagaagcta tttttaccac
     6961 gcaaagtcgc gatccgctgg ctcctctcag tgaagcacaa accttactca ctgttcactg
     7021 gtatctgcga aatcatacgc tctaccgtgc tgttcgtact tctgtggatg gcaggaagga
     7081 tcaacccgcc caggcgatgc tggaacatgt cgagagcttt cttctggaaa gtaacagcgg
     7141 ggaaagccag gaacttcctc tctcagtcac gttacatctg cagactcagc aatatggggg
     7201 gttacagcgc cgttttgcat tacctgagca gttagcgaga gaagagtcgc cagctcagac
     7261 gcaggccggg aataataacc atgaataatg aacaacgcgg cgtcgcgctg ctcattgtac
     7321 tcatgttact ggcgctcatg gctgctctgg cagcagacat gacgctcagt tttcacagcc
     7381 agcttcagcg aacccgccag gtaaaccatc atctgcaacg gcaatatgat attgaactgg
     7441 cggagaaact tgcgcttgcc agcctcaccc aggacgttaa agataacgac agacagacca
     7501 cgttgcagca gtactgggca cagccacaac agttgcagct ggaagatggc aatacagtga
     7561 agtggcagtt acgcgatgcc cagcactgtt ttaatctcaa tgcgttagca aaaatctctg
     7621 acgacccgct ggcttcccct gatttccccg cccaggtatt cagtgcgctg ctgattaacg
     7681 ccggtatcga tcgcggaaac accgatgaga tcgtgcaatc aatcgctgac tatattgatg
     7741 tcgatgactc accacgcttt cacggcgcag aagatagctt ttaccaaagc cagacgccgc
     7801 cccggcatag tgccaatcag atgctttttc tgactggcga attacgtcag ataaaaggca
     7861 taaccgaaaa catctatcag cggcttatcc cgtatgtctg cgttctcccg accacggaac
     7921 ttagcatcaa tcttaatatg ctaacggaga acgatattcc gctcttcaga gcgttattcc
     7981 tcaataacat cacggatgct gacgctcggg tcttattgca aaaaaggccg agggaaggct
     8041 ggctaaccac ggatgcattt ctttactggg cgcagcagga tttctccggt gtgaagccac
     8101 tggttgctca ggtgaaaagg catctctttc cctatagccg ctattttacg ctgagtacgg
     8161 aaagtatcag cgacgaacaa tcccagggat ggcaaagcca tatttttttc aaccgtaaac
     8221 agcagagcgc ccaaatctat cggcgtacgc tgcaactcta ctaatcataa ggatcgtgat
     8281 gcctgaatcg ttgatggtca tacgttcttc ctcgacgctg cgtaagcatt gggaatggat
     8341 gacgttctcc gcagatagcg tatcttcggt acatacgctc acggacgatc ttccgctgga
     8401 gtcgctggcc gatcagcccg gtgccggaaa tgttcatctt ttgatcccac cagagggcct
     8461 tttatatcgt tccctcacgc tgcccaacgc caaatacaaa ctcaccgcac aaacattaca
     8521 atggctggca gaggagacgt tacccgacaa cacccaggac tggcactgga cggttgtcga
     8581 taaacaaaac gaaagtgtcg aggtgatagg cattcagtcg gagaaactca gtcgctatct
     8641 tgagcgttta catactgccg gactcaacgt gacgcgggtt ttgccagatg gctgctatct
     8701 tccctgggaa gtagatagct ggactctggt taaccagcaa aaaagctggc tcattcgcag
     8761 tgccgcacat gccttcaacg aactggatga acactggtta cagcatcttg ctgcccagtt
     8821 tccgcctgag aatatgcttt gttatggtgt agtacctcac ggcgttgcag cggccaatcc
     8881 acttatccag catcctgaaa taccatcact gagtttgtat tctgccgata ttgcattcca
     8941 gcgctatgac atgctgcatg gtatttttcg taaacagaaa actgtaagca aatcgggaaa
     9001 atggctcgcc cgcctggcag taagctgtct cgttctcgcc attctttctt ttgttggcag
     9061 ccggagcatc gcgctctggc atacgcttaa gattgaagat caacttcagc aacaacagca
     9121 agaaacctgg caacgttatt tcccgcagat caaacgcacc cacaactttc atttttactt
     9181 taagcagcaa ctcgctcagc aatatcctga agctgtaccg ttgctctatc atttacaaac
     9241 acttctgctg gaacaccctg aactgcagtt aatggaggcg aactatagcc agaagcaaaa
     9301 gtcgctgacc ttaaaaatga gtgctaagag tgaagccaat atcgatcgtt tctgtgagtt
     9361 aacacagtcc tggcttccga tggaaaaaac agaaaaagat ccggtcagtg gtgtatggac
     9421 agtaaggaac tcagggaaat gattaaatca tggtgggcag aaaaatccac atcggaaaaa
     9481 cagatcgttg cggcactggc ggttctctcg ctcggcgttt tttgttggct tggggtgata
     9541 aaaccgatag atacctacat tgcagagcat cagtcccacg cgcaaaaaat taagaaggac
     9601 atcaaatgga tgcaggatca agcgagcacc catggtttac tcggtcatcc tgcgcttacg
     9661 cagcccatca aaaatatcct tctcgaggaa gcaaaacgtg agaatttggc tatcacgctg
     9721 gaaaacggcc cggacaatac tctgacaatt catcctgtca cagctcccct ctgaaaacgt
     9781 ctcccgctgg ctcactacgg cacaggtaac atacggtatt gttattgaag accttcagtt
     9841 tactcttgcc ggaaacgaag agatcaccct caggcatttg tccttcaggg agcaacaata
     9901 atgacaatgc tactgccgct cttcattctg gttggtttta ttgcaggtta ttttgttaat
     9961 gccatcgcct atcacctctc gccgttggag gataaaacgg cgttaacgtt tcgccaggta
    10021 ttggttcatt tcaggcaaaa aaaatatgcc tggcatgata cagtgcccct gatactttgt
    10081 gttgctgctg ctatcgcctg cgccctggca cctttcacgc ccatcgtgac tggcgcactc
    10141 tttctctatt tctgtttcgt actcacgctc agtgttattg attttcgcac tcagctcctg
    10201 cccgacaaac tcaccttacc gctgctctgg cttggcttgg tatttaatgc gcagtatgga
    10261 ttaattgatt tacatgatgc ggtttacggc gcggtagcgg gctatggggt gctgtggtgt
    10321 gtttactggg gcgtctggtt agtttgtcac aaagagggat tgggctacgg tgatttcaag
    10381 ctactggccg ctgcaggcgc atggtgtggc tggcaaacgt tgccaatgat actgctgatt
    10441 gcctcgctgg gtggcattgg ttacgccatc gtttcacaac ttctgcaacg ccggactata
    10501 accactatcg cattcggccc gtggcttgcg ctcggcagca tgataaacct ggggtatctg
    10561 gcctggatct cttattaacc gggagggttc tccctcccga cacggctcaa ccttcttcgc
    10621 ggatctgtgc ttgcagataa ttttgcaggc ccatcttctg aatcagatca agttccgttt
    10681 ccagccagtc gatatggcct tcttcatcac gcaaaatttc tatcatcata tcgcggctga
    10741 cgtaatcatg aacgctatcg gcataaccaa ttgcctcacg caaattcttc gcgccatcca
    10801 gctcaagtgc cagatcagaa cgcagcattt cctcaacatc ttcaccaatg ttcagtttgc
    10861 ccaggtcctg taagtttgga agaccttcca gaaaaagaat gcgctcaata taacgatcgg
    10921 cgtgtttcat ctcatcaatg gattcatgat actccacatc attgagacgt ttgagacccc
    10981 agtttttaaa cattcgggca tggagaaagt actgattgat tgcgacaagc tcatttccca
    11041 acagtttgtt gagataattt ataactttag tatcaccttt cattttttga ctccttcgct
    11101 tccactcttt gaagagtaga acggcttacg agtaagtcaa aaaacacact ccgtttatgc
    11161 ggactcctta aactccggca gctgcattaa ttcatcctcc atcacttcac gcgcggcccg
    11221 gacacattta ccgcactgat tgccgaccgg aataaatttt tttaattgct ggaacgagtg
    11281 aggggaaaac tggcgcacag cctgacgaat ttttttatcg ctgataccat tacaaagaca
    11341 aacgtacata gagactcccg tatactttct tcaaaaataa atgaaaatag ttcttatttc
    11401 aattacggaa agactctttt ttaagcaaag aatagaaata tgaataaaaa taatgggcta
    11461 ccaaaagtag cccattgaca aaaaatgcgg cgatactgga aggtatcgcc aacacattta
    11521 ttgcttagta aacggcgcga agatttcgct aaatgcgtac ttcggtgcct gttctttagt
    11581 caggccgtgg aattccggag acaggttagt gccgcccggc aggtcgcgcg cgattgacca
    11641 gataccaacc ataccgagat tacgcttctg cgcatcctgc atgaccagac gagcatcaga
    11701 gagatagaac acctcgccct gaacgtcgtt cacgccaacc atcggcgtgg tacccatcat
    11761 agcgtcaatt tctgcatcgc tcttattggg atggaggcct ttcaattgtg aatgcaggtt
    11821 ggcaatcgca gacgttgcac acttaccgtg aatgttctgg ccttcggtat ttgcagactg
    11881 acaaatcgcg ttaccgtagt ccattgtcat cacgttcaca cccgccagct caacaccttt
    11941 cgctttggca tcgctcagga cattcatccc ttccggcgtc aggccagtcg gcagaattgg
    12001 caaggtgtac cagatagcaa tatctttgcc ttctgacttc catttatcct gcactttctt
    12061 cacagcaagg ttacgacgtt caatagatgc ctgatccgca acccaggtgc cttcgatatc
    12121 gaagtccagg actttgaggt tcaggttatc aacgatgtca taataatgct gcatcagatc
    12181 gtctacgttc ttacaggaag cagccagcgg agcgttgtta gcaccaccga tagacagcat
    12241 cacatcgccg ccagcctcac gcagagcttt gattttgctg tactgagcgt aattctgcat
    12301 accataagcg gtaccccatg tcggcagaca ggtgttcgca tctttactca ccacaaacgc
    12361 cagcgtgaag tggttgacgt tatgattctt agccagcgca gccaaatccg ggatcgtatt
    12421 cagcgtaaag tcaacatacg gtgcataaac gtgtttcggc catgctttct tcggcgtacc
    12481 tacacgttct ttggttccgg tccagtcaac aaaaacacgc cacgggttgc tgtcagaagg
    12541 agaaactttc tgcactttac tctgagaaat gtagttcaca ccgttaaagc gaatcagcgt
    12601 atcgctggca taaagcgttt ctggattaaa ctgcggcgca ttattcagct cttcgttgct
    12661 atagctctga gccttaccta acggcttcca cgggcggcta ttgctaccgg tggcgttttg
    12721 gttcgcaacc agagcagggt tgtcactttg cgtccagaac agcgcttcat aagcctgccc
    12781 atcaacaaca acgcgatcac ctttcacgta cacagtggat gcggaccatg ctttcgccgg
    12841 ttcgtatttc ttccacgggt tatcgccgcc cgggacaaag ccccatgcgt cttgatcgac
    12901 agaagtttta taatgtgcat cgttataatc tacgatgctg tccgcgctgt acgctttgct
    12961 ggcctggaaa ccatccgcaa caatgacgcc gccgttatca atttcacagg tagtcgggtt
    13021 accgtactga ctcaattccg cagcggtagc tgtacgcacg agacgccatg ggttattgga
    13081 gttttcagcg ctggtacccc gtgggcaatc tttagaccca acccaccagg cgttcttgta
    13141 aacattacca ttgaaaataa catggtaaat ttcgctacct tcctggccgc tccacgtttg
    13201 caatgagtaa tcgttaccgc tatccgctgg tgttgatggc tcgttgttat ccggggttgg
    13261 ggtcaccggt gtttccggag ttggatcaac cggagtcaca gcaccgccat tattatccgg
    13321 cttaacggaa caggagccag ggttaccata ctggctgatt tctgttgccg ttgccgcgcg
    13381 aacataacgc catgggttac tggcatcgtt ttctttcgca tcgcctgggc aatttgccgc
    13441 gcctacccac caggcgttct tataaatgcc gccgttatag ataacgtacc atgtagaaga
    13501 accttcctga cctttccagg ctactacagc atagttgtcg tttgacttat cagctggggc
    13561 tggctgaaca tcaggagccg gtttaacgtc cggtgccggt ttcacatccg gtgaaggctg
    13621 tggtgctgaa gtacaggact gtggattact ggtttctgag atttccgttg ccgtagcggc
    13681 acgaacataa cgccatgggt tgctggcatc attgcttttc gcatcacccg gacagttaga
    13741 agaggctacc caccaggcat ttttatatac cgcaccatta aagacgacat accaggtctg
    13801 accgccctgc tgtttattcc aggcaactac agaagaatta gacggaacag tgccctgtgc
    13861 tggtgtagcc gaaccgccat tagccggcgt attggatgca ggcgtatttg aatttgaaga
    13921 agatgagctg ccgctctttt cgcaggaaag tgtattgcca aactgactaa tttcagcagc
    13981 tgttgcggta cgctttaaac gccacgggtt agttgcatca tttgctttcg cttttcccgg
    14041 gcaatttgta gaagaaaccc accaggcatt ttcataaatt ttgccatcga aaataacctg
    14101 atatttatta ccaccttgtt ggttattcca tgcttccatt gccaatgctg gcagagcgga
    14161 acacaccag
//
LOCUS       DAJOWB010000119        13950 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJOWB010000119 DAJOWB010000000
VERSION     DAJOWB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.119"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1148
                     /locus_tag="OHO85_003817"
     CDS             <1..1148
                     /locus_tag="OHO85_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCQ2673132.1"
                     /translation="LSLFKMDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKHFI
                     DIALYANNKCDDRITRRVYLSKEKEVSIKVVYFINNVAVHNNTIEIPQTVNGGYDFSH
                     LSLKGIVIKDEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNS
                     AQIKMADGTLNACSAMHVQFYNAAMNRANIKNTFLDYSNFYIAYMAEVNLYKVIAPYV
                     NLFKADLSFSKLDLINFEHADLSRVNLNKAILQSINLIDSKLFCTWLTNTFLEMVICT
                     GSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENI
                     PIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(1302..2951)
                     /locus_tag="OHO85_003818"
     CDS             complement(1302..2951)
                     /locus_tag="OHO85_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCQ2673133.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(3102..4451)
                     /gene="ghxP"
                     /locus_tag="OHO85_003819"
                     /gene_synonym="yjcD"
     CDS             complement(3102..4451)
                     /gene="ghxP"
                     /locus_tag="OHO85_003819"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCQ2673134.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4997..5461)
                     /gene="soxR"
                     /locus_tag="OHO85_003820"
     CDS             complement(4997..5461)
                     /gene="soxR"
                     /locus_tag="OHO85_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCQ2673135.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5547..5870
                     /gene="soxS"
                     /locus_tag="OHO85_003821"
     CDS             5547..5870
                     /gene="soxS"
                     /locus_tag="OHO85_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCQ2673136.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5873..7459)
                     /gene="pdeC"
                     /locus_tag="OHO85_003822"
     CDS             complement(5873..7459)
                     /gene="pdeC"
                     /locus_tag="OHO85_003822"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCQ2673137.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7889..8170
                     /locus_tag="OHO85_003823"
     CDS             7889..8170
                     /locus_tag="OHO85_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCQ2673138.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(8269..8805)
                     /gene="ssb1"
                     /locus_tag="OHO85_003824"
     CDS             complement(8269..8805)
                     /gene="ssb1"
                     /locus_tag="OHO85_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCQ2673139.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            9059..11881
                     /gene="uvrA"
                     /locus_tag="OHO85_003825"
     CDS             9059..11881
                     /gene="uvrA"
                     /locus_tag="OHO85_003825"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCQ2673140.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11916..12272)
                     /locus_tag="OHO85_003826"
     CDS             complement(11916..12272)
                     /locus_tag="OHO85_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCQ2673141.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(12276..12692)
                     /locus_tag="OHO85_003827"
     CDS             complement(12276..12692)
                     /locus_tag="OHO85_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCQ2673142.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12803..13516)
                     /gene="aphA"
                     /locus_tag="OHO85_003828"
     CDS             complement(12803..13516)
                     /gene="aphA"
                     /locus_tag="OHO85_003828"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCQ2673143.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPVT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVDARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3699 a         3557 c         3351 g         3343 t
ORIGIN      
        1 ccctctctct tttcaagatg gatgatcatg aaaaagtgat aggtttgatt cagaaaatga
       61 aaagaattta tgatagttta ccatcaggaa aaatcacgaa ggaaacggac aggaaaatac
      121 ataaacattt tatagatata gctttatatg caaataataa atgtgacgat agaattacga
      181 gaagagttta ccttagtaaa gaaaaggaag tatccattaa ggtggtatat tttataaata
      241 atgtcgccgt ccataataat actatcgaaa ttccacagac agtaaatggt ggttacgatt
      301 tttcacacct tagcctgaaa ggtatcgtga ttaaagatga agatttatcc aattcgaatt
      361 ttgcaggttg cagactacaa aacgctattt tccaggactg taatatgtat aaaacgaatt
      421 tttattacgc cataatggaa aaaatacttt ttgataattg tattctcgat gactcaaatt
      481 ccgctcagat aaaaatggcc gacggaactc taaatgcatg ctccgctatg catgttcaat
      541 tctacaatgc agcaatgaat agagccaata ttaaaaatac ctttcttgac tattcaaatt
      601 tttatatagc gtacatggct gaggtaaatc tttataaagt aatagcgcca tatgttaatt
      661 tatttaaagc cgaccttagt ttctctaaac tcgatttaat taactttgaa catgctgatc
      721 tgtctcgcgt caatctgaac aaagcaatcc tccagagtat aaacttaatt gatagcaaac
      781 tcttttgtac gtggctaaca aatacattcc tcgaaatggt tatatgtacc ggctctaata
      841 tggctaatgt taattttaat aatgccaatt taagcaactg ccatttcaac tgttctattt
      901 taacaaaagc ctgtatgttt aatacccgtc tctatcgggt taattttgat gaggctagcg
      961 tccagggaat gggcatttct attctccgtg gggaggaaaa tatccccatt gatagtgata
     1021 ccctggtaac actacagaaa ttctttgaag aagattgtac ctctcatact ggcatgtcac
     1081 aaactgagga taatattaat gcagtcgcta tgaagattac tgcagatatt atgcaacacg
     1141 cagattgagg ttaagtcgaa gaacagccaa tgaattgact ttacaagaga gataacttat
     1201 ttgttttaga atcatgaatg ttagcccgag ctaacctgtt aaaggtgcaa aagattgcac
     1261 ctttttcaga ttacctcggg cctggttacg tcgctttcgg cttactgatt ttcctcaatt
     1321 agcaacgctt caagcaaatc gagatcgtgc agtaattttt gcagcgtttc gttgctgatc
     1381 tcccgcgtgg cgcgcaggtg gtacagttca gcacgttcag aacgcaatgc cgccagacgg
     1441 aagcgacgct caaggttctc ttcctgcacg gaactttcaa cgtcgttacg tccatcggcg
     1501 cgacgacgca ggttaccaat gacgcgagaa ctgacctccg taagcagctg gttatcgata
     1561 ttctcttcgg tatccgccgc cagacgctcc tccattttct ggatcgccac aatcgccact
     1621 tctgccgttg ccgctcgcgc aatacgttct tctttcaatt gttgcgaatg gtccgcgact
     1681 tcaatgtgtt gtagcagaat aggcaacatc accacgccga caaacagcga gaagagaatg
     1741 acgccagccg ccaggaacac cagctcatag cgcgccggga agacgttacc atccggcaag
     1801 agcagcggga tagagagcac accggccaga gtgatcgccc cacgcacccc ggcgaaagac
     1861 gcgatcagga tttctcgtgt ggtccacgaa ccaaactcca tcggcttctt cttcaggaag
     1921 cggttgctga actttttcat cgtccacagc cagccgaaac ggaccagcat cagcgccgca
     1981 tatatcagaa taatatcggt aaacagcatc cagatttcga cgttagggtc gatttctgcc
     2041 gccatcagcg acgtctccag aatacccggc agctgcagac ctaacagcag gaacaccatg
     2101 ccgttaaaga caaattccag catcgcccag gtgctgtttg cacgcaggcg cattgccagc
     2161 ggcgcacggc gcatcacacc ggagcgggtg atggtcatcc cggcggcaac cgcagcgagg
     2221 atgccggaaa cgccaatatg ttcggcaatc agataagaag cgaatggcag caagaacagc
     2281 agaacaatct gcgtcgcggg ttcatcaccg ccccagcggc tgaggaatcg cagcgagcga
     2341 ccatacagcc agctcaccac aaaaccggca agaataccgc caatggctac tttcataaat
     2401 tcgaccgtcg caccgccgac ggtgaagatc atcgtcccca tcgccactgc cacggcaaac
     2461 ttcaacgaca ccaggccgga agcgtcattc atcaacgctt cgccctgcaa aatgcccatg
     2521 atttttttcg ggatgcgccc ttcgccaaca atcccggaga gcgccacagc atcggtcgga
     2581 gaaagtaccg ccgccagcgc aaaggcgggg atcagcggaa tgcccggcac cacccagtaa
     2641 ataaggaagc caatgccgac cacggtgacc accaccagcg ccagcgcgag gccgaaaatc
     2701 tctcgaccat gttcgagaaa ttcacgggtc ggcgttttcc agccatcagc gaacagcaac
     2761 ggcgggataa acaagactaa aaagagttca ggatcaaact ccacatgcaa accaaacgtc
     2821 ggccacgcca gtagcgcacc gatggcgatt tgcataagcg gaagcgggat ctgaaagggc
     2881 atgacacgag tgaccacccc ggacagcgag accacgaggg tcattatcag tatggtgaag
     2941 aagatttcca tgcgttccct gtttgcgatg ttttttatga tctgagaaag acgttatgcc
     3001 tgaattctca ttttttcaga atatcgcaaa agctcacaca atgtgcggag aaaataaaaa
     3061 cgggcagcca ttgccacccg ttttcagaat ccgatgaaag attagatagc ccagccaccc
     3121 gcatagaagg tcaccagcgc cacggcgata acgaccgtac cgatgttcaa cttgcgccat
     3181 tcgccggaaa ccagacgacc aatcaccaga gtcgcgaagc cgatcatgat gcctgttacg
     3241 atgttacagg tcagcacgat gaataccgcc gtaaccagac ccgccatcgc atcaacaaaa
     3301 tcagcaaagt cgattttcgc cacgttgctc agcatcagca ggccaacgta catcagcgcc
     3361 ggagccgttg cataccccgg aacgaggtaa gagagcggag agaggaacag aatcagcagg
     3421 aacagcacgc caacggtgat agccgtcaaa ccggttttac cgcccgccgc cgtacccgcc
     3481 gcagactcga tgtataccgc tgccggagct gcacccacca ggccagagaa aacgctgctc
     3541 atggagtcag tggtcagtgc tttcccaccg tcgataattt gcccatcttt atccagcagg
     3601 ttcgcctggc cggcgacggc acggatagtt ccggtggcat caaatactgc cgtcattacc
     3661 agcgccagaa cgcttggcag gactacagga ttcagcgcgc ccataatgtc caggctgcca
     3721 atcagtgagt tgccgttttc atcgctcaat gaaggcatgg cgaaaacgcc ggagaaatgg
     3781 acgttaggat cgaagatcaa accgacaatt gagataccga taatggtcag cagaatgcca
     3841 ccagggactt tcagtttttc caggccgatg atcaccgcca gacctaccag tgacataatc
     3901 accgggaagg tcgcgaaatc acccagcgca acgggcagac catcaagcgg gtttttaatc
     3961 accagaccga caccgttagc ggcaatgagc agcaggaaca gaccgatacc aatccccgtg
     4021 ccgtgcgcca caccgtgcgg caagttgcgc aaaatccagc tacggatacc cgtggcagaa
     4081 attaccgtaa acagcacacc catcaggaac acggcaccca gcgcgacagg tacgctaata
     4141 tgttgcccca gcaccaggct gaatgcggta aatgccgtca gggagatggc gcaaccaatc
     4201 gccaacggca gattagccca cagacccatc acgatagaac cgagtccggc aaccagacag
     4261 gttgcaacga aaactgccgc aggcgggaag cctgctttac ccaacatacc tggaacgacg
     4321 atgaccgagt agaccatcgc cagaaacgtt gttaacccgg caaccacttc ctgacggaca
     4381 gtgcttccac gttgtgaaat tttaaaccag gcgtcgagtg aaccgccggt acgcgctgat
     4441 ggcgtagaca taagaaaaca tcccctgaga gtttaatttt cgtcaagatg cgtggcggac
     4501 aatcccctgc cagcgaaacg ttatcttcct gctccaggtt tgcgacaatt tgctgcgtcg
     4561 caaaaaagca aacgtttagc tccgcgcata caaaacgggt ggtaaaaaaa gcaaacgatt
     4621 atccggcgat catagcggct ttttcaaccg aagtttgctg cttttttctc ttttattgtc
     4681 ttgacgatga aaaaccaggc aattgatgcg caaacgttat cgtcggtgcg caagattgca
     4741 gcgttatcga gccgtatacc gttcaggatt gcgataaaaa atcgtctgag atgacaaaaa
     4801 tattgcggtt aaaaagtata cttttacact ggcgaagaaa cgtcggtgac atacaattaa
     4861 agcatcaaca ccaaccggaa cctccaccac gtgctcgaat gaggtgtgtt gacgtcgggg
     4921 gaaaccctcc tgtgtaccag cgggatagag agaaagacaa agaccggaaa acaaactaaa
     4981 gcgcccttgt ggcgctttag ttttgttcat cttccagcaa gcgtgcgccg gtaccttctt
     5041 ctcctaagcg gtcgcccggg ttacgcaacg ggcaatcact gcgcgaaagg cagccacaac
     5101 caatacatcc gtccagttcg tcacgcagcg ccactaaggt atgaatgcgc cgatccaact
     5161 cttctcgcca ttgggacgaa agctgtttcc actctttcgc acttaacgta tgcccttcgg
     5221 gcaacacgcc aaacgcttca ccaatggtcg ccagcggaat gccaatacgc tgagcaattt
     5281 tgataattgc aacatatcgc aacacatcac gtttatatcg ccgctgattg ccgctgttac
     5341 ggatactggt aatcaaccct ttactttcat agaaatgcag cgccgatacc gccacaccgc
     5401 tgcgtttcgc cacttcgccg ggggttagca gcgctttaat gcggggtaat ttcttttcca
     5461 taaatcgctt tacctcaagt taacttgagg aattatactc cccaacagat gaattaacga
     5521 actgaacact gaaaagaggc agatttatgt cccatcagaa aattattcag gatcttatcg
     5581 catggattga cgagcatatt gaccagccgc ttaacattga tgtagtcgca aaaaaatcag
     5641 gctattcaaa gtggtacttg caacgaatgt tccgcacggt gacgcatcag acgcttggcg
     5701 attacattcg ccaacgccgc ctgttactgg ccgccgttga gttgcgcacc accgagcgtc
     5761 cgatttttga tatcgcaatg gacctgggtt atgtctcgca gcagaccttc tcccgcgttt
     5821 tccgtcggca gtttgatcgc actcccagcg attatcgcca ccgcctgtaa ttttattgcc
     5881 cgcgcgttaa ctcccgcgcg ggtaattgct ccatccattg cataaacacc tgcggcggca
     5941 tcgccttcgc aaagaaccat ccctggcaat agcgcacgcc gcgtttgcgc agccagttaa
     6001 cctgctcctc agtttcgacg ccttcagcga tcgtttttaa ccccaggctg tgcgccagct
     6061 cgatgatgtg ttccgcaatc aaatgactgg ttttgtgggt ggtcagcgtt tcaacaaacg
     6121 atttgtcgat tttcaaaata tcgacattca atgatttaag gttatgcaag ttagagtagc
     6181 caataccaaa atcatcaatt gccacttcgt aacctgcctg gcggaaagcc agaataatcg
     6241 gcgtcatttt gtcaacatca agaaatgcat gttcagtcac ttcaaattta atttgctgcg
     6301 gacgcaccgc gtattgctct gttttctgat tgattcgcgc tatcaaccgt gacgtatgaa
     6361 aatcggaggc cgacaggtta atagaaacat agcgatctgc atgtgttgcc aggtaatcgc
     6421 ccagatcgcg gaagacatta tcaataacat aatcagttat ctgttctatc atcccctcct
     6481 tttctgccag cggaataaac tctgccggat tcattatttg cccctgctca ccaagccaac
     6541 gtaacaacgc ttcagcgccg atacattttt ctgttttgat atcgattatt ggctggtaat
     6601 aaagacaaag ttgatgtttt tcgagggcgc gttgcaattt acgtttggga gataaatagt
     6661 tttgtcgaat acgtagccag agtaatagca gaacaagact ccccaaaata ccggcgggca
     6721 acgcaaaaat aagatgatta taaaaatggg ttataagacg ttgatatgaa gtcgcaacaa
     6781 tggctgcaat tgggcgtttt gtcgaataaa ctgtcgcata taaatagcca tttctttgta
     6841 cggttaaatc cttcaaatga atcagcggag aaaacgttgc tgccgaggcc tctttgctta
     6901 acgagaaaaa ggttttcgtc accgtatcat acacacccca ttgcaatgtc gggtcatcag
     6961 acatcacttc actccagaag agagggttga taaccgccac ataatttccc cgctgcatat
     7021 aggtcatttt atagccagag aaaaaaggcg tatcgcggta ataatagata gaaacgttag
     7081 gttcacgctt ataatcggcc ggtgcaatcg tatagccgtt tacaggcgct atcagcgatg
     7141 agcataaaaa atggttatca cgggcataga tcaattcatt aatataaaga tagccacgaa
     7201 taatattcaa cattcgcttt tgatgggctg gagtgcaaac ttgcccctga tagcgttcag
     7261 ctgcatcgct cactaaatct gcctggcgaa tgaccagctc ggatttatcc agtgccagtt
     7321 gagcaaaggt tcggagctga ttattcactt ctgattttgc ccaaaggaag gcaatccata
     7381 gcgaaagaat gatgggaaag agaactaaaa agataatgcc cggcaacgcc agtaattggt
     7441 gtcgtgcacg atgactcatt gctatcaatt tcctgcctgt ttatacccgg ccattgcaag
     7501 gtgaaaaccc tgggtaccga cgtctttaga taaatcttac ggtataaata gcacttttgc
     7561 ctgatttgaa attgatacct ttctaatcgt cacctggatg aaaataattc attgatattc
     7621 aaaattaatt tcctacagga atgcagaggc ggcgggaaga tatgccctta atgccactga
     7681 aaaaccacat aatctgtaca gcaataaaag tcacggccta atatggctaa caaacaatca
     7741 attgtgatat agttcacaaa attaatgaaa caaacagagt gtttcatttt tgtgttctgc
     7801 aagccccgcg atagccagag tgtaccacgc ctcccgtgaa caacgccgcg ctgtccaggg
     7861 catcggctct tttcagagga attgtttgat ggctaccctc accactggcg tggttcttct
     7921 tcgctggcaa cttcttagtg ccgtaatgat gtttctggcc agcacactca acatccgttt
     7981 tcgtcggtct gattatgtcg ggcttgcggt gatcagcagc ggtctgggcg ttgtttccgc
     8041 ctgttggttc gcaatggggt tgcttggcat cacaatggcg gacatcaccg ctatctggca
     8101 caacatcgag tcggtgatga tagaagagat gaatcagaca ccgccacaat ggccaatgat
     8161 tttgacctga tacgtagaag cctccataac ggaggcttct tttttatggc tggcagatgc
     8221 tttaatcatc caccttaaaa caatataacc tattgtttta atgacaaatc agaacggaat
     8281 gtcatcatca aagtccatcg gcggctcgtt agacggcgct gccggagcgg actgctgcgg
     8341 gcgagactgc gcgccgccgc tgaactgatt gccaccctgc ggctgctgtg gctgacccca
     8401 accgccctgc ggctgaccac caccgatatt gccacctgcc ggagcgccac caccctgacg
     8461 accacccagc atctgcatgg tgccgccaac gttcaccacg acttctgtgg tgtagcgatc
     8521 ctgaccggat tgatcggtcc atttacgggt acgcagctga ccttcgatat aaacctgaga
     8581 acctttacgc agatattcgc tggccacttc tgccagtttg ccgaacagca caacgcggtg
     8641 ccattcagtc tgttctttca tctcgccggt cgctttatca cgccaggatt cggaagtagc
     8701 cagcgtaatg ttggcaactg cgccaccatt tggcatgtag cgtacttccg ggtcctgacc
     8761 cagattacca acgagaataa ccttgtttac gcctctgctg gccatgttcg tgtctcctga
     8821 aaaaaatcgt tctgaataag tgtaaacgcg cgattgtacc attaccaata gcgcttttac
     8881 tatgttgtga cctcggttcc gggaaacaaa cctggccaga cattgttaca caacactccg
     8941 ggtaatgcat tccaatactg tatattcatt caggtcaatt tgtgtcataa ttaaccgttt
     9001 gtgatcgccg gtagcaccat gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat
     9061 ggataagatc gaagttcggg gcgcccgcac ccataatctc aaaaacatca acctcgttat
     9121 cccccgcgac aagctcattg tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc
     9181 tttcgacacc ttatatgccg aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc
     9241 gcggcagttt ctgtcactga tggaaaagcc ggacgtcgat catattgagg ggctttctcc
     9301 tgccatctca attgagcaga aatcgacgtc tcataacccg cgttctacgg tggggacaat
     9361 caccgaaatc cacgactatt tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc
     9421 ggaccacgac gtcccgctgg cggcgcaaac cgtcagccag atggtggata acgtgctgtc
     9481 gcagccggaa ggcaagcgtc tgatgctgct cgcgccaatc attaaagagc gcaaaggcga
     9541 acacaccaaa acgctggaga acctggcaag ccagggttac atccgtgctc gtattgatgg
     9601 cgaagtctgc gatctttccg atccgccgaa actggaactg caaaagaaac ataccattga
     9661 agtggtggtt gatcgcttca aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt
     9721 tgaaaccgcg ctggagcttt ccggtggtac agcggtagtg gcggatatgg acgacccgaa
     9781 agcggaagag ctgctgttct ccgccaactt cgcctgccca atttgcggct acagtatgcg
     9841 tgaactggag ccgcgactgt tttcgtttaa caacccggca ggtgcctgcc cgacctgtga
     9901 cggccttggc gtacagcaat atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc
     9961 actggctggc ggtgcgatcc gtggctggga tcgccgcaac ttctattact tccagatgct
    10021 gaaatcgctg gcagatcact ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc
    10081 gaacgtgcat aaagtggtgt tgtacggttc tggcaaagaa aacattgaat tcaaatacat
    10141 gaacgatcgt ggcgatacct ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat
    10201 ggagcgccgt tataaagaga cagaatccag tgcggtacgt gaagaattag ccaagtttat
    10261 cagcaatcgc ccatgcgcca gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt
    10321 ttatgtcgag aatacgccgc tgcctgctat ctccgacatg agcatcggtc atgcgatgga
    10381 attcttcaac aatctcaaac tcgctggtca acgggcgaag attgcggaaa aaatccttaa
    10441 agagatcggc gatcgcctga aattcctcgt taacgtcggt ctgaattacc tgacactttc
    10501 ccgctcggca gagacacttt ccggcggtga agcccagcgt attcgtctgg cgagccagat
    10561 tggtgcgggc ctggttggcg ttatgtacgt gctggacgag ccgtctatcg gcctgcacca
    10621 gcgcgataac gagcgcctgt tgggtacgct tatccatctg cgcgatctcg gtaataccgt
    10681 gattgtggtg gagcacgacg aagacgcgat tcgcgcggct gaccatgtga tcgatatcgg
    10741 tcctggtgcg ggtgttcacg gcggtgaagt ggtcgcggaa ggtccactgg aagcgattat
    10801 ggcggtgccg gagtcgttaa ccggacagta catgagcggt aaacgcaaga ttgaagtgcc
    10861 gaagaaacgc gttccggcaa atccggaaaa agtgctgaag ctgacaggcg cacgcggcaa
    10921 caacctgaag gacgtgacgc tcacgctgcc agtcggtctg tttacctgca tcacaggggt
    10981 ttcaggttcc ggtaaatcga cgctgattaa cgacacactg ttcccgattg cccaacgcca
    11041 gttgaatggt gcgaccatcg ccgaaccggc accgtatcgc gatattcagg ggctggagca
    11101 tttcgataaa gtgatcgata tcgaccaaag cccaattggt cgtactccac gttctaaccc
    11161 ggcgacctat accggcgtgt ttacacctgt gcgcgaactg tttgcgggcg taccggaatc
    11221 ccgtgcgcgt ggttatacgc caggacgttt cagctttaac gtccgtggcg ggcgctgcga
    11281 agcctgtcag ggcgacggtg tgatcaaagt ggagatgcac ttcctgccgg atatctacgt
    11341 gccgtgcgat cagtgcaaag gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa
    11401 aggcaaaacc atccacgaag tgctggatat gaccatcgaa gaggcgcgtg agttctttga
    11461 tgcggtgcca gctctggcgc gtaagctgca aacgttgatg gacgttggtc tgacgtacat
    11521 tcgcctgggg cagtccgcaa ccacactttc tggtggtgaa gcccagcgcg tgaagctggc
    11581 gcgtgagctg tcaaaacgcg gcaccgggca gacgctgtat attctcgacg agccgaccac
    11641 cggtctgcac ttcgccgata ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca
    11701 gggcaatacc attgtggtga ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat
    11761 tgtcgacctg ggaccagaag gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc
    11821 agaaaccgtc gcggagtgcg aagcttcgca tacggcacgc ttcctcaagc cgatgctgta
    11881 atcgttaagg ccgctttctg agcggccttt tcctttcaga gttgtaccag caatttacgc
    11941 ttttcttccg gtagtaaatt cactgcctgc tgataagacg catccaccag ataatagatt
    12001 tgcgaatccg gcagcgaacc gtcgagatac acggtgctcc agtgcgcttt attcagatgg
    12061 cggctaggac gcacatcgct gtgctgctga cgtagcagct ccgccagctc cgggctggtt
    12121 ttcagcgaaa cagctgggcg attttctact tctttcacca tcgcaaacag tacatcttcc
    12181 actttgatct gcgtcgcttt ccagtcgtta tgcacgctct gttctgcgcc tggttttgcc
    12241 atgcaatatt gtagcaactc cgaaatggtc attttttact ccccttgtag tgtcgcgatg
    12301 atgcgacgcg atccgccgtg gatgcgatgt tcccccagcc agatgccttg ccaggtgccg
    12361 gtctgaatac gccctttatg taccggcaat acaagcgatg ttcccagcat tgaggatttg
    12421 atatgagaag gcatatcgtc tgctccctca tagtcatgct cataatttcc gttgtcggga
    12481 acggtgcgga ggaaaaaacg ctccatgtcg tggcgtacgg tgggatcgca gttctcatta
    12541 agtgtcagag aggcggaggt atgttgcagc aacagatgca gtaagccgat gttaacgcgc
    12601 ggcatatcag ccagctgatt cagaatttca tccgttacca gatgaaaccc acgagatttg
    12661 gcgctaagcg tgagcgtctt ttgataccac atgtgctgct ccttgataaa actctcttaa
    12721 tcagtttgca gcaagacagc gaaaggataa aggtgtgatt aaaaaaacag cattcgggag
    12781 agcgtcacgc tctcccgctc tgtcagtatt ctgaattgac gatcacctct tcaccaaacg
    12841 cacccgcttg tggcaagggt ttgtaggtag agttggaggc gcgcagaatg cggataccac
    12901 gagcgtcgac atcgcgtgcg gcggtaatat cgttatcgga atcgccataa aaaattcgga
    12961 tatttttatc ctgcagccat tgcgattttg tattttgccc tggtttatcg cccgcaaaga
    13021 tcaccggatt catgttggtg acaggaatat gaaaattatc cgccagcgtt tttgaaaccg
    13081 tttctgtttt cgtcgggcta cgaccagtca caaagaagat cgcgtcaccg cggcgtacat
    13141 gcatatcaat cagctggcga gcgacctctt ttggaatgct gaattcatcc cagccattgt
    13201 tcattttttc ccagaacaca ggatttttca gataatcttc gctttctggt gagaaggttt
    13261 ttttgccgcg ccagaagccc gggctggaaa aaagtaccgt gtcatcgata tcaaacccca
    13321 ccgccattgg cggacgccct gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa
    13381 ttggtgcctg ttcagcaagc ctggcaacgt tagtcccagg gttaagcggt gaaggagatg
    13441 aggccagggc aacagcggaa ctgtttagag cgaacaataa gcaaacggca ctgattgcct
    13501 gtgtgatctt gcgcatattt ttccctaaat agtcagtatg ttgaaacttt ttattgtgag
    13561 atttgttgca aaaaccatct gaccataacg ccagctgccg gggaaaggaa ggattatctg
    13621 cggtttttgt gatgaaaagc aggatgatta tgtttaaccc agtaaatacg ttcctgtctt
    13681 tttttgtata aaaaatgttt attactcgat gtaaggatac attaatttta cgtttattat
    13741 gatgaatcct ctgcaaatga gcgaaatttg atagaacatt tatctgctgt acgatttaat
    13801 gaacttaatg atacagcatt acaaaaacaa tcgaagttta taaagatgat tcctgattga
    13861 ccagaaccct atcctacaat ggtgactaat tttattactc ccgatggaga tgatgatatg
    13921 aatatatcat atgtgaatag caacaaaacg
//
LOCUS       DAJOWB010000120        13808 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJOWB010000120 DAJOWB010000000
VERSION     DAJOWB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.120"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..717)
                     /gene="yecC"
                     /locus_tag="OHO85_003829"
     CDS             complement(<1..717)
                     /gene="yecC"
                     /locus_tag="OHO85_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCQ2673144.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPEQPR"
     gene            complement(714..1382)
                     /gene="tcyL"
                     /locus_tag="OHO85_003830"
     CDS             complement(714..1382)
                     /gene="tcyL"
                     /locus_tag="OHO85_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCQ2673145.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1397..2383)
                     /gene="dcyD"
                     /locus_tag="OHO85_003831"
     CDS             complement(1397..2383)
                     /gene="dcyD"
                     /locus_tag="OHO85_003831"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCQ2673146.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2488..3288)
                     /gene="tcyJ"
                     /locus_tag="OHO85_003832"
     CDS             complement(2488..3288)
                     /gene="tcyJ"
                     /locus_tag="OHO85_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2673147.1"
                     /translation="MKLAHLGRQALMSVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHFGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3376..3927)
                     /gene="fliZ"
                     /locus_tag="OHO85_003833"
     CDS             complement(3376..3927)
                     /gene="fliZ"
                     /locus_tag="OHO85_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCQ2673148.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDALLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(3973..4692)
                     /gene="fliA"
                     /locus_tag="OHO85_003834"
     CDS             complement(3973..4692)
                     /gene="fliA"
                     /locus_tag="OHO85_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCQ2673149.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(4856..5905)
                     /locus_tag="OHO85_003835"
     CDS             complement(4856..5905)
                     /locus_tag="OHO85_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079755.1"
                     /note="structural flagella protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCQ2673150.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSTSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISLAQTAEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKNDSMKIQIGANDNQTISIGL
                     QQIDSTTLNLKGFTVSGMADFSAAKLTAADGTAIAAADVKDAGGKQVNLLSYTDTASN
                     STKYAVVDSATGKYMEATVVITGTAAAVTVGAAEVAGAATADPLKALDAAIAKVDKFR
                     SSLGAVQNRLDSAVTNLNNTTTNLSEAPSRIQDADYATEVSNMSKAQIIQQAGNSVLS
                     KANQVPQQVLSLLQG"
     gene            6153..7568
                     /gene="fliD"
                     /locus_tag="OHO85_003836"
     CDS             6153..7568
                     /gene="fliD"
                     /locus_tag="OHO85_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCQ2673151.1"
                     /translation="MASISTLGVGSGLDLSSILDSLEAAEKSTLTPISKQQSSYTAKL
                     SAYGTLKSALESFQTANTALNKADLFTATSTTSSSSAFSATTTGSAIAGKYTISVSQL
                     AQAQTLTTKNSQKDSKAAIATSDSVLTIQQGGGKDPVTIDISAGNSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSITANDTGSNNAMKLSVSGDSALESFMGYNGTPGDSSNGM
                     IESVTAQNAKLTVNNVEIENSSNTISDSLEDITLNLNDVTTGNQTLTISKDTSKAENA
                     VKAWVDAYNTLQDTFSSLTKYTAVDAGAESQDSSNGALLGDSTLRTIQTQLKTLLANT
                     HSSSNYKTLAQIGITSDASTGKLEIATDKLQTALKNDAAGIGEMFIGDGKSTGVTTGI
                     SNNLTSWLSSTGIIQAAKDGVSKTLNNLTDQYNAASERIDTLMTRYKAQFTQLDVLMN
                     SLNSTSSYLTQQFDTSNSNSK"
     gene            7584..7994
                     /gene="fliS"
                     /locus_tag="OHO85_003837"
     CDS             7584..7994
                     /gene="fliS"
                     /locus_tag="OHO85_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCQ2673152.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            7994..8359
                     /gene="fliT"
                     /locus_tag="OHO85_003838"
     CDS             7994..8359
                     /gene="fliT"
                     /locus_tag="OHO85_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCQ2673153.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELITSEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            8437..9924
                     /gene="amyA"
                     /locus_tag="OHO85_003839"
     CDS             8437..9924
                     /gene="amyA"
                     /locus_tag="OHO85_003839"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCQ2673154.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(9958..10371)
                     /locus_tag="OHO85_003840"
     CDS             complement(9958..10371)
                     /locus_tag="OHO85_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCQ2673155.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            10558..11763
                     /gene="yedE"
                     /locus_tag="OHO85_003841"
     CDS             10558..11763
                     /gene="yedE"
                     /locus_tag="OHO85_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCQ2673156.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGLLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            11760..11993
                     /gene="yedF"
                     /locus_tag="OHO85_003842"
     CDS             11760..11993
                     /gene="yedF"
                     /locus_tag="OHO85_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCQ2673157.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12102..12599
                     /locus_tag="OHO85_003843"
     CDS             12102..12599
                     /locus_tag="OHO85_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000334561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCQ2673158.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSHMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQAVFIAAIGSTPFERGDEAGRSFCKPAQGLSVTVAMA
                     VRANL"
     gene            complement(12481..12810)
                     /locus_tag="OHO85_003844"
     CDS             complement(12481..12810)
                     /locus_tag="OHO85_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2673159.1"
                     /translation="MKRLQAFKFRLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDPERAYKNFFRLRHHAQT
                     VCYLSRL"
     gene            12833..13357
                     /gene="tnpA"
                     /locus_tag="OHO85_003845"
     CDS             12833..13357
                     /gene="tnpA"
                     /locus_tag="OHO85_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCQ2673160.1"
                     /translation="MKKETDIRRGRHCVFLKHVHLVFVTKYRRQIFDHDATEKLRTYF
                     SNVCADFEAERVEMDGGPDHVHLLINYPPKLAISSLVNSLKGVSSRLLRRDRPDIAVR
                     YYDKGVLWSPGYFVSSCILTYQLDLITTISAPNRISNVISIPLIIPSTSCNFPTLELH
                     IVQTPDAKKSHVHF"
     gene            complement(13514..>13808)
                     /locus_tag="OHO85_003846"
     CDS             complement(13514..>13808)
                     /locus_tag="OHO85_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2673161.1"
                     /translation="NLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDD
                     VRLNNALSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT         3400 a         3549 c         3416 g         3443 t
ORIGIN      
        1 gcgaggctgc tcgggatcgg caaataacgc ttttgcggcc ccctgctcga ctatccgccc
       61 ctggtccata aagatcgccc ggtccgcaac atcccgggca aagctcattt cgtgcgttac
      121 aatcaccatc gtgcgctttt cctgcgccag ctgacggatg gtgttcagga cttcgcccac
      181 cagctctgga tccagcgccg acgttggctc gtcaaacaaa atcacctcag gacgcattgc
      241 cagcgcacgt gcaatcgcaa cacgttgctg ttgaccgcca gacaaacgac gtggatagct
      301 ggtttcttta ccggccagcc caactttcgc caacagctcg cgagcgcgcg ccgtggcctc
      361 ttctttcggt tcgcctttga cgatcaccgg cccttcaata atgttttcca gcaccgtacg
      421 atgcggaaac aaattaaagt tctggaagac aaacccgacg tgctgacgca actggcgaat
      481 cagcgatttt tgctgactta atgaacgtgc agtatcaata gtgatatcgc caacggtgat
      541 cgttcccgct tcgggttgtt ccagcagatt tatgctgcgt agcaacgtgg ttttgccgga
      601 accactcgga ccgataattg ccaccacttc gccaggcttt acctcaaggt cgataccgtg
      661 cagcaccgtc tgaccgtgaa attttttcac caggttctta acttcaatgg cactcatttt
      721 ggttctctct cctggcgatt aagttgattc tcaaaatggt tctgcaacgt cgataatact
      781 gtcgccatga tccagtagat gagcgaagcc gccaggtaca tggtgaacac ctccagcgtg
      841 cgcgaggtaa tcaactgcgc ttgacggaac agctccggca cctggattgt cgcggccagc
      901 gacgtatctt ttaccaggct gataaagctg ttcgacagcg gcggcaacgc cacccgcgcc
      961 gcctgtggca aaatcgcgcg acgcatggtc tgccacggcg tcataccaat actggcagct
     1021 gcttcccact gacctttatc aatagaagaa atggcggcgc gaagcgtttc ggcagcatag
     1081 gcggcagtgt tgagcgacag accaatcatt gctgacggaa tgggatctaa ttcgatacca
     1141 aactgcggca ggccgtaata aatcataaac agttgtgcga tgagcggcgt accacgaaaa
     1201 atagagatat aaaaacgcgc cagccagcgc acgggccaga tcggcgacag gcgcatcagc
     1261 gcgagaataa acccgagcag taagccaaaa aacatgccgc cgatactgag ttgcagcgta
     1321 tacccagccc ctttcagcag gaacggcaag gaatcaataa ccagttgtat actttcttgc
     1381 atgagcgttt ccggagctaa acgtggggat gataggcgaa cagcgcaggc gcgccgccgg
     1441 tatgaataaa cagaatcggc ccttcatctt tgaagcgttt ctgactgata ccgtcaatca
     1501 gccccgccat cgcttttccg gtatacacag gatcaagcag aatgccttcc agccgcgcca
     1561 gcaatttcac tgcttccatg ccttcatcgt tcggcacgcc gtagccaggt gcaaaatagt
     1621 catcccagag taaaatttcc gctgatgcgg tcagctccag ttctttcgca atcgcctgtt
     1681 gtaggttaac cactttcggc aattgatcgg caacggaacg cgacacggta acgccaatca
     1741 gttcgctttc aggcataagg tgttccagcc caacagccag tccggcgtga gttccggcac
     1801 tgcccgatgc gaccaccacc gacgaaatat taaccgcccc ttcacactgt tgcgcgattt
     1861 ccagcgcact ctccacataa cccagcgcgc ccagagcatt agaaccgcca accggaatga
     1921 catacgggcg aaagccttgt gcttcgactc gcgtcgccag ctcttccagt tgggcattgg
     1981 gatcggtcag tgcgtcacac atttcaatct gggtattgaa cagatccagc aacaaacgat
     2041 tgccgttggt taaatagttt tctgcggttg tgccaatagg attttccagc agcgccacgc
     2101 agtgcagacc gagtttcgcc gcaactgcgg cagtctggcg cacatggtta gactggatcg
     2161 ccccggcagt aatcagcgta tcggcacctt cgcgcagagc atctgctgcg agaaattcca
     2221 gcttacgtaa tttattgccg cccattgcca tgggggtgac gtcatcccgt ttgatgaaaa
     2281 tttcccgtcc aagataatca gaaaagcgcg gcagatattc gagcggagtt ggcgcgccga
     2341 taaactccag ccgtggaaaa cgggttaaat tatgcagtgg cataacagcc tccgatgtgt
     2401 gttgttgtga ttttcttatt atgcacgctg aaaacgcgta aataaaaaag gcgctagtga
     2461 aagcgccctt ttttgtcatt atgctgatta tttggtcaca tcagcaccaa accatttttc
     2521 ggaaagagct tgcagagtgc cgtctttttg catttccgca attgcatcat tcaccgcttt
     2581 cagcaggtct tcatttcctt tacgcagcgc cacgccagac tcctgacggg agaatgcttc
     2641 accggttact gccagcgtat cgttggtttt cttcaccaga tccagcgccg ccagacgatc
     2701 aacgaggatc gcatcgatac ggcctacgcg cagatcctga tatttggttg ggtcatcatc
     2761 ataggtacgc acatcgacac cctgaacatt ctgccgcagc cactcttcat agttggtgcc
     2821 caggccgaca cccacttttt tgcctttcag atcagcagcc gttttaatgg tgccttcgtt
     2881 accttttttc accagcgcct gaataccgga aatggtgtaa ggggtagaga aatcgtattt
     2941 tttcttgcgc tcgtcagaaa tggtgacctg attaatcacc acatcaatgc gtttagagtc
     3001 cagcgacgcc agcataccgt cccatttggt cggttttagt gacgcctcaa cgccaaaatg
     3061 ttttgccagc tgttgggcaa attccacttc aaaaccggtt aatttgccgt catccccctg
     3121 aaaactgaac ggcggataag tgccttccag ccctaccagc agcgtgccgc gctctttaac
     3181 tttattaagc agaccttcat ctgcaaaact tttaacgctc atgcccgcaa ccagcgctac
     3241 ggccatcaca ctcatcaatg cctgacgtcc cagatgtgct aatttcatat ttaccccgaa
     3301 tgttgttatg tctgtttgca gtgtagagcc attgtttgta aacacaaaaa caactccgct
     3361 acatcttatt cttatttaat atatatcaga agcaggcagg ctggaggatt tctgcacaag
     3421 tccggtacac gtttgacgct gatagtgttg atacttccgt aatgcgatgc ggtaattgtt
     3481 ggtgctggtg gtgggcagcc acggggcgag gatttcatca aggaaaccgt cctgcagcgg
     3541 atcgagggaa atattttgct cgtgtagatg attccccaaa cggcgcaaac gaacgacata
     3601 ttcacgcacc gtacctgggc tcatttcagt ttgctcaaaa agaaattgct taaagccgat
     3661 aatatcgaaa aaatcactct gcgttttgca atgtaaatca ccgcaaaatc ggcacaatgc
     3721 cgcccatgat ttttgttccg tttgccagcc attttcatca agcagcgcgt ccaggcggga
     3781 aatctcgatt ttattcacta ttttgccgtc gcgaacgagg gtaatgcgat cgagtaattt
     3841 acggcaatgc gcgcaatgag tctggctgtg tttaaagtct ttaagatagc ggcttaatgg
     3901 ccgtcttttc aggtgctgca ccatcattaa gaactcctgg tagtcaaagt taaagtgcgg
     3961 catttactga cgttataact tacccagttt agtgcgtaac cgtttaatag cctggctgtg
     4021 taactgactg acccgcgatt ccccgacctc cagcaccgcg ccaatctctt tgagattcag
     4081 ctcttcctgg taatagaggg ttaataccag tttttcgcgc tccggcaacg tttcgatggc
     4141 ttccatcacc cgctgacgca gattactgtc cagcagttgt tgtagcgggt tttctcgctg
     4201 atgatcatca gtaaccagtt cgatgctatc gccgtgctct tcgcgccact catcgtagga
     4261 gaagagctgg ctgttattgg tgtcgagcaa catttggcga taatcggcaa tatcgatccc
     4321 taaacgttcc gctacctcag tttccgtggc gttgcggcca agttcctgct ccagttgccc
     4381 tattgcctgt gccacttcac gcgcgttgcg tcgcacgctg cgcggcaccc agtcacggct
     4441 gcgaagttca tccagcatag cgccacggat acgctgcact gcgtaagttg taaatgccgt
     4501 tccttgtagg gcgtcatagc gttcgacggc attaagtaac ccaatgccgc ccgcctgtag
     4561 cagatcgtca agttccacgc tcgcgggcag tcgaacctgc aggcgcaatg cttcgtgacg
     4621 caccagcggg acataacgct gccacagcga gtgtttatcc attacacctt cagcggtata
     4681 gagtgaattc acgataaaca gccctgcgtt atatgagtta tcggcatgat tatccgtttc
     4741 tgcagggttt ttaatcggac gattagtggg tgaaatgagg ggttatttgg gggttaccgg
     4801 taaattgcgg gcagaaaaaa ccccgccggt agcggggtca ggcaggttaa ggccattagc
     4861 cttgtaacag agacagaact tgctgcggta cctggttggc tttagacagc acggagttgc
     4921 ccgcctgctg gataatctgc gctttcgaca tgttggacac ttcggtcgca tagtcggcgt
     4981 cctgaatacg ggacggcgct tcagacaggt tggtggtggt gttgttcagg ttggtgaccg
     5041 cagaatccag acggttttga acggcaccga gggaggagcg gaatttgtcg actttagcga
     5101 ttgcggcatc cagtgctttt aacggatcgg ctgtagcggc tcccgccact tccgctgcac
     5161 caacagttac cgccgccgcc gtaccggtaa tgactacagt ggcttccatg tatttaccgg
     5221 ttgcagaatc aacgaccgcg tatttagtac tgttagacgc ggtgtcagtg taagacagta
     5281 aattgacttg tttaccccca gcatccttga catccgcagc agcaattgct gtaccatcag
     5341 cagccgtcag tttcgccgcg ctgaaatccg ccatgccgga cacggtaaat cctttcagat
     5401 tcaaagtggt actgtcgatt tgttgcaagc caatgctgat cgtctggtta tcattggcac
     5461 caatctgaat cttcatcgaa tcgtttttcg acagcacgtt cacaccgttg aactgggtct
     5521 gaccagatac acggtcaatt tcatcaagac gggatttgat ttcgtcctga atagaagaca
     5581 ggtcggaatc agagttcgtg ccggtagagg cctgaacggt cagttcacga atacgctgca
     5641 agttgttgtt aatctctgac agcgcgcctt cagccgtctg cgccagagaa ataccgtcgt
     5701 tggcgttacg tgcggcctga gtcagacctt tgatgttaga agtaaagcgg ttagcaatcg
     5761 cctggcccgc ggcgtcatct ttagcgctgt taatacgcag accagaagag aggcgctcga
     5821 tagaagtcga cagcgcagac tggtttttgt tgatgttgtt ctgagtgatc agcgagaggc
     5881 tgttggtatt aatgacttgt gccataattc attttcctgt tttcaagtct acggtttaag
     5941 tattgggctt ccacccttcg gcttcgtcgc cgtcaaccct gttatcgtct ggtcactcac
     6001 aacctttaga atttttttct agcggtcgaa atactcccct tcctcgttgc tgttcgtgcc
     6061 attaaggcaa aaaataaatc gtaaacttta ccccgcttac gccgataacc ccggtattcg
     6121 ttctatacac gtcgcatgat taaggaaact atatggctag tatttcaacg cttggggttg
     6181 ggtcggggct ggacttaagc agcatccttg acagtctgga ggcggcagaa aaatctaccc
     6241 tgacgccgat atcaaaacag caaagctctt acaccgcgaa actgagcgcc tacgggacgc
     6301 tgaaaagtgc actagagagt ttccagacag ccaacacagc actgaataaa gccgatttat
     6361 ttaccgccac ctcaaccacc agcagttcat cggctttcag cgccaccacc acgggcagcg
     6421 cgattgctgg gaaatacacc attagcgtta gccaactggc gcaggcacag acgctgacaa
     6481 caaaaaatag tcaaaaagac agtaaagccg ccatcgccac cagcgatagc gtactgacca
     6541 tccaacaagg cggcggtaaa gatccggtca cgattgatat cagcgcgggt aactcgtcac
     6601 tttccggcat tcgcgatgcc attaacaacg ccaaagctgg tgtcagcgcc agtatcatca
     6661 atgtgggaaa cggcgaatat cgtctgtcca tcaccgctaa cgatacgggc agcaataacg
     6721 ctatgaaact tagcgtcagc ggcgacagcg ccctggaaag ttttatgggt tacaacggaa
     6781 cgccgggtga tagcagcaat ggcatgattg aaagcgtcac cgcgcaaaac gccaaactga
     6841 cagtcaataa cgttgagatt gaaaacagca gtaacactat cagcgactca ctggaagata
     6901 tcaccctcaa cctgaatgac gtcaccaccg gtaatcagac actgaccatc agtaaagaca
     6961 catccaaagc tgaaaatgcg gttaaagcgt gggtggatgc ctataacaca ttgcaggata
     7021 cattcagcag cttaaccaaa tacaccgctg tcgatgccgg agctgaaagt caggactcca
     7081 gtaatggcgc attactcggt gactccacac tgcgtaccat ccagactcag cttaaaacct
     7141 tgctcgccaa tactcacagc agttcaaact ataaaacgct ggcacagatt ggcattacca
     7201 gcgatgccag taccggcaag ctggaaatcg cgaccgacaa actgcaaacg gcgctaaaaa
     7261 atgatgccgc tggtatcggt gaaatgttta taggtgacgg caaaagtact ggggtaacca
     7321 ccggcattag taacaacctg accagttggc tctcttccac cgggataatc caggcagcaa
     7381 aagatggcgt cagcaaaacg ctgaataatt tgaccgacca gtacaacgca gccagtgaac
     7441 gtatcgatac cttaatgacg cgctataaag cccagttcac ccaattagat gtgttaatga
     7501 actcgctgaa ctccactagt agctatctga cacagcagtt cgacacctca aatagcaact
     7561 cgaaataatc ttcaggagag actatgtacg cggcaaaagg cacccaggcc tatgcacaaa
     7621 ttggcgtcga aagcgccgta atgagcgcca gccaacagca gctggtcacc atgctatttg
     7681 atggagtgct gagcgcactg gttagagcgc gcctgtttat gcaggacaac aatcagcaag
     7741 gcaaaggcgt ctctttatca aaagcgatca acatcattga gaacggactg cgggtgagtc
     7801 ttgatgaaga gagcaaagac gaattaaccc aaaacttgat tgctctttat agctatatgg
     7861 tcaggcgctt gctgcaagcc aatttacgta acgatgtctc cgcagtcgaa gaagtggaag
     7921 cattaatgcg caatattgcc gatgcctgga aagagtcttt actctcccct tctttgattc
     7981 aggacccagt ctgatgaaca atgcaccgca tttgtatttc gcctggcaac aactcgtcga
     8041 aaaaagccag ctcatgttac gcctggcaac ggaagaacaa tgggacgaac tcatcaccag
     8101 cgaaatggcg tatgtgaatg cggtgcagga gatcgcacac ctgactgaag aggttgcccc
     8161 gtccactacg atgcaggagc agctccgccc gatgctgcgc ctgattctcg acaacgaaag
     8221 caaggtaaaa cagttattac agattcggat ggatgaactg gcgaaactgg tcggtcagtc
     8281 atcggtgcaa aaatcggtgt taagtgccta tggcgatcag ggcggctttg tgctggctcc
     8341 gcaggataac ctcttttgaa tcttaatgag ccgatggctc gcgaataatc cgattacggc
     8401 tacgcttcta atattcccct tgaatggagt cgaagaatgc gtaatcccac gctgttacaa
     8461 tgttttcact ggtattaccc ggaaggcggt aagctctggc ctgaactggc cgagcgcgca
     8521 gacggtttta atgatattgg tatcaatatg gtctggttgc cgcccgccta taaaggcgca
     8581 tcgggcggct attcggtcgg ctacgactcc tatgatttat ttgatctagg tgagtttgat
     8641 cagaaaggca gcatccctac taaatatggt gataaagcac aactgctggc cgccattgac
     8701 gccctgaaac gtaatgacat tgcggtgctg ctggacgtgg tagtcaacca caaaatgggc
     8761 gcggatgaaa aagaagctat tcgcgtgcag cgtgtaaatg ctgatgaccg tacgcaaatt
     8821 gacgaagaaa tcattgagtg tgaaggctgg acgcgttaca ccttccccgc tcgtgccggg
     8881 caatactcgc agtttatctg ggatttcaaa tgttttagcg gtatcgacca tatcgaaaac
     8941 cctgatgaag atggcatttt taaaattgtt aacgactata ccggagaagg ctggaacgat
     9001 caggttgatg atgaactcgg taatttcgat tatctgatgg gggagaatat cgattttcgc
     9061 aatcatgccg tgacggaaga gattaaatac tgggcgcgct gggtgatgga acaaacgcaa
     9121 tgcgacggct ttcgtcttga tgcggtcaaa catattccgg catggtttta taaagagtgg
     9181 atcgaacacg tacaggaagt tgcgccaaag ccgttgttta ttgtggctga atactggtcg
     9241 catgaagtcg ataaactgca aacgtatatt gatcaggtgg aaggcaaaac catgctgttt
     9301 gatgcgccgc tgcagatgaa attccatgaa gcatcgcgca tgggtcgcga ctacgacatg
     9361 acgcagatct tcactgggac gctggtggaa gccgatcctt ttcacgccgt aaccctcgtt
     9421 gccaaccacg acacccaacc gttgcaagcc ctcgaagccc ccgtcgaacc gtggtttaaa
     9481 ccgctggcgt atgccttaat tttgttgcgg gaaaatggcg ttccttcggt gttctatccg
     9541 gacctctacg gtgcacatta cgaagatgtc ggtggtgacg ggcaaaccta tccgatagat
     9601 atgccaataa tcgaacagct tgatgagtta attctcgccc gtcagcgttt cgcccacggt
     9661 gtacagacgt tatttttcga ccatccgaac tgcattgcct ttagccgcag tggcaccgac
     9721 gaatatcccg gctgcgtggt ggtcatgtcg aacggagatg atggcgaaaa aaccattcat
     9781 ctgggagaga attacggcaa taaaacctgg cgtgattttc tggggaaccg gcaagagagc
     9841 gtagtgacag acgaaaacgg cgaagcgacc ttcttttgca acggcggcag cgtcagcgtg
     9901 tgggttatcg aagaggtgat ttaaatccat ccccggcggc aagccgggga gatttcatta
     9961 cggcagtggc gtcggtaacg gcgctttatc cagcgcggcg gcacactctg ccgtcgggcg
    10021 atccacacgc tccatcgtca taccatcgta ctcaatggtg ttaccgtcac gctcaacctc
    10081 gtacagctca cgttttacgg taacgttagt gagatcatca gacatcaatg tcaacttacc
    10141 cggcacggca atcacccgct gccactgacg gcaatccagg gtatcgccct ctttagtgac
    10201 aatgaggctg cctatcgctt ccggactgac caacgcacgt tgcggccctt tggtctgcca
    10261 gtagcccgcc agccagtccg gcgcgggggt tttcacgaca ttgttatagt tttcgacttc
    10321 ggcacagccc gccagcaaca tgagcgcacc tgcaattgcg agttttttca tcattcgtcc
    10381 tgcatgagaa gaaaagagga ttgtggcatt aaagccctaa tgtcgccagc ctttccaggt
    10441 tcagagagaa atttgatcta accattattt ttgtgaatta ttaccccgaa aataaatttg
    10501 catggtgtat gattcgcgac tcttgtttca aaccttctga gttcagaggc tacattcatg
    10561 tcatggcagc aattcaaaca cgcctggttg attaaattct gggcgcccat ccccgcggtc
    10621 atcgcggcgg gtattctctc cacttactat tttggcatca ctggcacctt ttgggctgtc
    10681 acaggtgaat ttacccgttg gggcggtcag ctcctgcagt tgttcggcgt ccatgctgaa
    10741 gagtggggtt attttaaaat tatccatctg gaaggatcgc cattaacccg catcgacgga
    10801 atgatgatcc tcggtatgtt tggcggctgt tttgccgcag cgctgtgggc caacaatgtc
    10861 aaactgcgca tgccgcgcag ccgtatccgc attatgcagg ccatcattgg cggtattatc
    10921 gccggttttg gcgcacgtct ggcaatgggc tgtaacctgg cggcgttctt taccggaatt
    10981 ccacagttct cgctgcacgc ctggttcttt gccatcgcca ctgccattgg ttcatggttt
    11041 ggcgcgcgct ttacccttct gcccatcttc cgtattcccg tgaaaatgca gaaagtttct
    11101 gctgcctcac cgttgacgca aaaaccggat caggcgcggc gtcgttttcg tctcgggatg
    11161 ctggtctttt tcggcctgct gggctgggcg ctgctgacgg cgatgaacca gcccaaactg
    11221 gggctggcaa tgctgtttgg cgtcggcttt ggtttactga ttgaacgtgc gcaaatctgc
    11281 tttacttcgg cgttccgcga tatgtggatc accggacgta cccatatggc gaaagcaatc
    11341 attatcggta tggcggtaag tgccatcggg atcttcagtt acgtacagtt aggcgttgaa
    11401 cccaaaatca tgtgggcggg accaaacgcg gtaattggtg gtttactgtt tggttttggc
    11461 atcgtgctgg caggcggctg cgaaaccggc tggatgtacc gcgcggtaga aggccaggtg
    11521 cactactggt gggtcggtct gggcaacgtg atcggctcaa cgattctggc gtactactgg
    11581 gatgatttcg ctccggcgct ggccaccgac tgggacaaaa tcaacctgct gaaaaccttt
    11641 ggcccgatgg gcggcctgct ggtgacatat ttgctgttgt ttgctgctct aatgttgatt
    11701 attggctggg aaaaacgctt cttccgccgt gcggcaccgc agactgctaa ggagatcgca
    11761 tgaaaaatat cgttcctgat taccgtcttg atatggtggg tgaaccctgc ccttatccgg
    11821 cagtcgcaac ccttgaggcg atgccgcagt taaaaaaagg ggagatcctg gaagtggtga
    11881 gcgactgtcc gcagtcgatc aataatattc cactggatgc gcgtaatcac ggctatacgg
    11941 tgctggatat tcagcaagac gggccgacca ttcgttattt aattcagaag taatccctca
    12001 ttcccgctgt ggcccacagc gggaatatcg ttgcctctct atcacataga tttaacccgc
    12061 cccctctcct attcttttca gtgtgacctc aggaggtttc tatgtgtgga cgctttgccc
    12121 aatcccaaac gcgtgaagat taccttgcgc ttctcgcgga agatattgaa cgcgatattc
    12181 cctacgatcc cgaacctatt ggcagataca acgtcgcgcc gggaaccaaa gtccttctgc
    12241 tcagtgaacg tgatgaacac cttcatctgg atccggtttt ctggggatat gctcccggat
    12301 ggtgggataa accgccgctg attaacgccc gcgtagaaac tgccgcaacc agtcacatgt
    12361 ttaaaccgct ctggcaacat ggtcgggcaa tctgttttgc cgatggctgg tttgagtgga
    12421 aaaaagaagg cgacaaaaag cagccttatt ttatctatcg tgctgatgga caagctgttt
    12481 ttatagccgc gataggtagc acaccgtttg agcgtggtga cgaagccgga agaagttttt
    12541 gtaagcccgc tcagggtctt tcagtgactg ttgcaatggc tgtgagggcg aatctttaag
    12601 ccattgcgtt tcagtggcat ttttccactc aaccaaccag gaagccattt tcccgtaagg
    12661 gatgtattta ttaccggcct catgattctc attctgaagc gccagagcac gattgaaaac
    12721 gaaacgacat gcgcctgcga agcgcctcat ttcacgctcc tgttgaccac ctggtcttaa
    12781 ccggaatttg aatgcctgta gtcgtttcat gggccaaatc atactttggc ctatgaaaaa
    12841 agaaaccgat atccgacgcg gaaggcattg tgttttcctg aagcatgttc acctagtctt
    12901 cgttactaaa taccgacgtc agatttttga ccatgacgcg acagaaaaac tacgcactta
    12961 cttttcaaat gtatgtgctg attttgaagc tgaacgggtt gaaatggatg gcggaccaga
    13021 tcacgtccat ttgttaatta actatcctcc caaactggcg atatccagtc tggtaaacag
    13081 cctcaaaggc gtatcgagta ggttactgcg acgagatcga ccagatattg cagtcaggta
    13141 ttacgacaaa ggcgttttgt ggagtcctgg ctattttgtc agtagctgca tcttaacgta
    13201 tcaactagat ttaataacaa cgatatccgc acctaaccgg atatctaatg tcataagcat
    13261 ccctttaatt atcccctcaa cctcttgtaa ctttcccact cttgagctcc acatagtcca
    13321 aacgccggat gcaaaaaaat cacacgtgca tttctgaaat tgtgatggag gaagatagta
    13381 tcaattatat gtgcttagaa acgtccccga ttagcgcaga agtcaccgga ttgttcagac
    13441 tccaataggt tgagtatgcc gtatttattt tccaaaataa aaatcgatgc tgttgctcct
    13501 tcataaaata cttttactct ctggacgctt ttcttagctc tcttggtgtt atcttaaaac
    13561 gattatggaa cctttcagta aaacgagaag gacacttgta accattttcc ctggcaatct
    13621 cgctgatggg ttttactgtc gtttgtatag cagacaacgc attatttaac ctcacgtcgt
    13681 ccaatatact ttgaaaactt actccctcgc ttgccagacg gcgatgcaat gtggaaacag
    13741 aaatgtagag atatctcgct accttgtttg ctgtccattt tgttccaggt tcggatagca
    13801 ttaggtta
//
LOCUS       DAJOWB010000121        13655 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJOWB010000121 DAJOWB010000000
VERSION     DAJOWB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.121"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            341..715
                     /gene="tomB"
                     /locus_tag="OHO85_003847"
     CDS             341..715
                     /gene="tomB"
                     /locus_tag="OHO85_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCQ2673162.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            741..959
                     /gene="hha"
                     /locus_tag="OHO85_003848"
     CDS             741..959
                     /gene="hha"
                     /locus_tag="OHO85_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCQ2673163.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            1131..1682
                     /gene="maa"
                     /locus_tag="OHO85_003849"
     CDS             1131..1682
                     /gene="maa"
                     /locus_tag="OHO85_003849"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCQ2673164.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPNNVVVGGNPARIIKKL"
     gene            1798..2268
                     /locus_tag="OHO85_003850"
     CDS             1798..2268
                     /locus_tag="OHO85_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCQ2673165.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            2432..3990
                     /gene="pdeB"
                     /locus_tag="OHO85_003851"
                     /pseudo
     CDS             2432..3990
                     /gene="pdeB"
                     /locus_tag="OHO85_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(4032..4385)
                     /locus_tag="OHO85_003852"
     CDS             complement(4032..4385)
                     /locus_tag="OHO85_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HCQ2673166.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKKFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(4505..4601)
                     /gene="ffs"
                     /locus_tag="OHO85_003853"
     ncRNA           complement(4505..4601)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OHO85_003853"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4764..5075
                     /locus_tag="OHO85_003854"
     CDS             4764..5075
                     /locus_tag="OHO85_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCQ2673167.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(5106..5678)
                     /locus_tag="OHO85_003855"
     CDS             complement(5106..5678)
                     /locus_tag="OHO85_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCQ2673168.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            5896..6756
                     /gene="tesB"
                     /locus_tag="OHO85_003856"
     CDS             5896..6756
                     /gene="tesB"
                     /locus_tag="OHO85_003856"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCQ2673169.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(6805..8091)
                     /gene="amtB"
                     /locus_tag="OHO85_003857"
     CDS             complement(6805..8091)
                     /gene="amtB"
                     /locus_tag="OHO85_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCQ2673170.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(8121..8459)
                     /gene="glnK"
                     /locus_tag="OHO85_003858"
     CDS             complement(8121..8459)
                     /gene="glnK"
                     /locus_tag="OHO85_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCQ2673171.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(8640..10421)
                     /locus_tag="OHO85_003859"
     CDS             complement(8640..10421)
                     /locus_tag="OHO85_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCQ2673172.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(10414..12186)
                     /locus_tag="OHO85_003860"
     CDS             complement(10414..12186)
                     /locus_tag="OHO85_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCQ2673173.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(12216..12674)
                     /gene="decR"
                     /locus_tag="OHO85_003861"
     CDS             complement(12216..12674)
                     /gene="decR"
                     /locus_tag="OHO85_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCQ2673174.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(12828..13646)
                     /gene="cof"
                     /locus_tag="OHO85_003862"
     CDS             complement(12828..13646)
                     /gene="cof"
                     /locus_tag="OHO85_003862"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCQ2673175.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
BASE COUNT         3455 a         3681 c         3278 g         3241 t
ORIGIN      
        1 ttcttaatta tttgcggtaa ttctttattc attcctcggt tattacgtca tattcagagc
       61 aatcctggta ttagtgtcac caatttcatc tggcgataat cctgaaatgt tatgaacagt
      121 tcgagcaaac tgcttttacc tgctgcgggt tagtgctagt atgaaaaagt gagtcctgtc
      181 ccgattcctt cctaattgta atttttcgta ataatgcgat gaaaacctgc aaagagtggc
      241 ttatagttaa gctaacaaac gagagggcaa gtccaggtca gtaagttttt tccatcccga
      301 aaggtgtccg ttagttcaac cgctaagaag gggacgcgtt atggatgaat actcacccaa
      361 aagacatgat atcgcacagc ttaagtttct ctgtgaaacc ctgtatcatg actgccttgc
      421 aaaccttgaa gaaagcaatc atggctgggt taacgaccca acctcggcga tcaacctcca
      481 gttgaatgaa ctgattgagc atattgcgac cttcgcactt aattacaaaa ttaagtataa
      541 tgaagacaat aagctcattg agcagatcga cgaatatctg gatgacacct ttatgttgtt
      601 cagtagttat ggtattaata tgcaggatct tcagaaatgg cggaagtcag gtaatcgact
      661 attccgttgt tttgtcaatg cgacgaaaga gaatcctgcg agtttatctt gttagaatta
      721 ttacaaccat gggtagaagt atgtccgaaa aacctttaac gaaaaccgat tatttaatgc
      781 gtttacgtcg ttgccagaca attgacacgc tggagcgtgt tatcgagaaa aataaatacg
      841 aattatcaga taatgaactg gcggtatttt actcagccgc agatcaccgc ctcgccgaat
      901 tgaccatgaa taaactgtac gacaagatcc cttcctcagt ttggaaattt attcgctaat
      961 aaataattcg ctttcggagc tataaccggc tgtttattaa gaattttata ctttttcgcc
     1021 atgaagacat accctatgtg atctttatca cacagatgta atgggaacgt tctcttcact
     1081 gacttttcgt cttactgtgt tgccgcattt tcagcaaccg gaggtcagta atgagcacag
     1141 aaaaagaaaa gatgattgct ggtgagttgt atcgctcggc agatgagacg ttatctcgcg
     1201 atcgcctgcg cgctcgtcag cttattcacc gatacaatca ttccctggcg gaagagcaca
     1261 cattacgcca gcaaattctc gctgatctat tcggtcaggt gacagaggct tatattgagc
     1321 caacgtttcg ctgtgactat ggctataaca tttttctcgg taataatttt ttcgccaact
     1381 tcgattgcgt gatgcttgat gtctgcccta ttcgcatcgg tgataactgt atgttggcac
     1441 caggcgttca tatctacacg gcaacacatc ccatcgaccc tgtagcacgt aatagcggtg
     1501 ctgaactggg gaaacccgtc accatcggta ataacgtctg gattggcgga cgcgcggtca
     1561 ttaaccctgg tgtgaccatt ggtgataacg tcgtggtagc ctcaggtgca gttgtcacaa
     1621 aagatgtccc gaacaacgtt gtcgtgggcg gtaatccagc cagaataatt aaaaaattgt
     1681 aatcggtttt tcgcaactgt tatgcaaaat tgtggtaaat ctgttacttc ccctctacta
     1741 ttcccacgtt aaaatagggt gttccctgga aagttgcaga taccacgaag gcaaacgatg
     1801 accgaaatac aacgcctgct gaccgaaacg attgagtctc tgaatacccg cgaaaaacgc
     1861 gacaacaaac cccgctttag tatcagtttt atccgtaaac atccggggct gtttatcggt
     1921 atgtacgttg ctttttttgc caccctggcg gtgatgttgc agtccgaaac gctttcaggc
     1981 tctgtctggc tactggttgt attatttatc ctgcttaatg gtttcttctt tttcgatgtc
     2041 tacccacgct accgctatga agatatcgac gtgctggatt tccgcgtttg ctataacggc
     2101 gaatggtaca acacgcgctt tgtacctgcc gcgctggttg aagccatctt gaactctccg
     2161 cgtgtcgcgg atgttcataa ggaacaactg caaaaaatga tcgtccgtaa aggtgaactg
     2221 tctttttacg atatttttac cctcgctcgc gccgaatcaa catcttaagt tagggttaca
     2281 taccaggcgt aaagctctgc gcctggtcaa atgacaatga tcgtttccac ccatcacttc
     2341 atgaaatacc agctctacct ccttatctcc tgccagcctt tttccacaat cagatatact
     2401 ttccctacac tgtgttaata aggatatgct ggtgagaaca cgacatctgg tcggccttat
     2461 ttcgggagta ctgattcttt cagtattgct gcctgtcggc ttaagcatct ggctggccca
     2521 tcagcaggta gaaacatcgt ttattgaaga gctggatacc tattcctccc gcgtcgctat
     2581 tcgggccaat aaggtggcga cacaagggaa agatgcgctg caagagctgg aaagatggca
     2641 aggcgctgcc tgtagcgaag cccatctcat ggaaatgcgt cgggtatctt acagttatcg
     2701 ctatattcag gaagtggttt atatcgataa caacgttccc cagtgttcgt ctctggagca
     2761 tgaaagtccg cccgatacct tccccgagcc aggtaaaatt tcgaaagatg gttatcgtgt
     2821 ctggttaaca tcgcataacg atttaggcat tatccgttac atggtcgcca tgggaacggc
     2881 acattatgtc gtcatgatcg accccgcttc ctttattgat gtcattccct atagctcatg
     2941 gcaaattgat gccgccatta ttggcaatgc ccataacgtt gtcataacca gcagcgatga
     3001 aattgctcag ggaattatta ccaggctaca aaaaacaccc ggtgagcata tcgaaaataa
     3061 tggaatcatt tacgatatcc tgcccttccc ggagatgaat atttcgatca tcacctgggc
     3121 ttcaacgaaa atgttgcaga aaggctggca tcggcaagtc tttatttggt taccgctcgg
     3181 gttggtgatt ggcctgctgg cagcgatgtt tgtgctgcgt attttgcgcc gtatccagtc
     3241 accgcatcat cggctgcagg atgctatcga aaatcgtgat atttgcgtgc actatcagcc
     3301 gattgtctcc ttagccaatg gcaaaattgt cggtgctgag gcactggcgc gctggccgca
     3361 gacagacggt agttggttgt caccagatag ttttattccg ctggcacagc aaacgggcct
     3421 ttctgagcca ttgacgctac tgattataag aagcgtcttt gaagatatgg gcgactggct
     3481 gcgtcagcat ccacagcagc atatttcgat caatcttgaa tccaccgtgc tcacctcgga
     3541 aaaaatcccg caattgctgc gtgaaatgat caatcactat caggttaatc ccagacagat
     3601 cgcgcttgaa ctcactgaac gcgagtttgc cgatccgaaa accagcgccc cgataatttc
     3661 tcgctaccgg gaggcgggcc atgaaattta tcttgatgat tttggtacgg gatattcaag
     3721 tttaagttat ttacaggatc tggatgtcga cattctgaag atcgataaat ctttcgttga
     3781 tgcgctggaa tataaaaatg tcacgccgca tatcatcgaa atggcaaaaa cactgaaact
     3841 gaaaatggta gcggagggaa tcgaaaccag taaaccagta aacaagaaga gtggctacgc
     3901 cagcatggcg tgcactacgg tcagggctgg ctctacagca aggcattacc gaaagaagat
     3961 ttcttacgct gggccgagca acatttgtga atttttgggc tgacggcacg ccgcacagcc
     4021 cgtcacgcta cctattcgtc gatgattgac tcgaatccgc catagatcat tcgcttgccg
     4081 tcaaacggca tggactcgcc gaactctttc atccgtgggt ccgacatcat cttttgatta
     4141 gcagcgtcgc ggacctcttt tgaagggtat tcaatccagc taaagacaac ctcttcattc
     4201 tcttccgctt tcaccgccat acgaaaatcg gtcactttgc catccggtac atcgctggcc
     4261 cagcattcga caatacgaag cgcgccaaac tttttaaaca atggcgcagc cttagcggcc
     4321 atttcccgat aggcatcctt tttatcggca ggaacggcaa ccacaaaacc atcaacatac
     4381 ttcatcagac atacctccga agcgtactgc gcagccagac aattcttgcg acgtcagttg
     4441 tggttaaagt ttagccatgt tggggttttt tgacgaaacg gtgggaggca gaaatgggtg
     4501 ggggccctgc cagctacatc ccggcacacg cgtcatctgc cttggctgct tccttccgga
     4561 cctgacctgg taaacagagt agcgttgcgg gagaaccaac agagccccca ttgagagcgt
     4621 tgagaaccaa cgcgcaggcg cattatcact gctgccccgg ctaattgcaa cccatcgcgt
     4681 gccagatgct ggtttcttgc gcaatgcgac ttcactcggg cgtttttcca gattatgctg
     4741 aaaaattacc tcaggaaaaa aagatggaaa aagaagattc atttccccaa cgcgtctggc
     4801 aaatcgtcgc cgctattccc gaaggctatg tcaccactta cggtgatgtg gcgaaactgg
     4861 cgggatcgcc ccgcgccgcg cgccaggtgg gcggtgtgtt aaagcgtctc cctgaaggca
     4921 gcaccttacc ctggcaccgg gtggttaatc gccacggcac aatttcgcta accggaccgg
     4981 atttacagcg tcagcgacag gcattactgg cagaaggtgt aatggtatcg ggaagcgggc
     5041 aaatcgactt gcagcgttat cgctggaact actaaccctc tccgggcgga gagggttaac
     5101 gtcgcttaat attgcgtagg agctggaact gccgaagacg gattcacctg agttggtgaa
     5161 gtcgaaggta cggtagtcgt tgcgccaccg ctggcctgaa ccggcacggc ggtttgctgt
     5221 accggcacca atgtcaggtc ggctttagtt ccgccctggt tgatcaccgg ctgaacggta
     5281 tcggtgataa ataccagttt gtcattcacg gtaatcgccg cactcaacag aatacgcgcg
     5341 ttcggctgaa catctgccgg gttaaatggc agaacaaagc tgaatggtga ctgtttacct
     5401 tcggtacgca ccgctttctg cgccagcact tttgacggtg catcggctaa cgacgcgtca
     5461 gaaagtgtca cggtcagcac agcatcaggc ggcagtgcga ctttctgacg gatccagacg
     5521 gtaccggaga cattcggttg ctggatagca ggttgttgtg ttgctgaaat agacgtattt
     5581 gcagccgggg ctggcgtctg aatatccgcg cttttatctg cgcaagccgc caatgcaatc
     5641 gcaaccgcta aaccactggc catgtgcacg agtttcattc atttctcctt attatcaatg
     5701 caccagcggg ctaactttcc tcgccggaag agtggttaac aaaatagtaa cgtcaacaag
     5761 tgtggcacac atcacgcatt tctgcctgta attagcccgt aattcagacc attgcaccca
     5821 tcggaccagt ggcaaatttg cgttatactc aactcacttt ggcttgctgc ggcagctttg
     5881 ttactggaga gttatatgag tcaggcgcta aaaaatttac tgacattgtt aaatctagaa
     5941 aaaattgagg aaggactctt tcgcggccag agtgaagatt taggtttacg ccaggtgttt
     6001 ggcggccagg tcgtgggtca ggccttgtat gctgcaaaag agaccgtccc tgaagagcgg
     6061 ctggtacatt cgtttcacag ctactttctt cgccctggcg atagtaagaa gccgattatt
     6121 tatgatgtcg aaacgctgcg tgacggtaac agcttcagcg cccgccgggt tgctgctatt
     6181 caaaacggca aaccgatttt ttatatgacg gcctctttcc aggcaccaga agcgggtttc
     6241 gaacatcaaa aaacaatgcc gtccgcgcca gcgcctgatg gcctgccttc ggaaacgcaa
     6301 atcgcccaat cgctggcgca cctgctgccg ccagtgctta aagataaatt catctgcgat
     6361 cgtccgctgg aagtccgtcc ggtggagttt cataacccac tgaaaggtca cgtcgcagaa
     6421 ccacatcgtc aggtgtggat ccgcgcaaat ggtagcgtgc cggatgacct gcgcgttcat
     6481 cagtatctgc tcggttacgc ttctgatctt aacttcctgc cggtagctct acagccgcac
     6541 ggcatcggtt ttctcgaacc agggattcag attgccacca ttgaccattc catgtggttc
     6601 catcgcccgt ttaatttgaa tgaatggctg ctgtatagcg tggagagcac ctcggcgtcc
     6661 agcgcacgcg gctttgtgcg cggtgagttt tatacccagg acggcgtgct ggttgcctcg
     6721 accgttcagg aaggggtaat gcgtaatcac aattaaaaaa atagccggag gtgaaaaccg
     6781 tccggctatt ttttgcagtg cttgttacgc gttataggca ttctcgccgt ggctgttgac
     6841 gtccagccct tctcgctctt gttcttccgg cacacgtagg ccaaccgtca gatccgccag
     6901 tttgtagcca ataaatgcca ccacaccgga ccagacgatc gtaatggcga tgctttccag
     6961 ctgtaccagc aactgatggc ccatcgtcac accttcagcg aagcccacgc cacccagcga
     7021 actggcggca aaaatcccag tcatgataca gccgacaatg ccacaaacgc cgtgcacacc
     7081 gaagacatcg cagggatcat ccacccgcag caagcgtttg agcatagtaa cgccccacaa
     7141 gcccgccaga ccagctacca cgccgataat caacgcgccg ccaaccccaa tgtagccaca
     7201 cgctggcgtc acgccgacca gaccggcaat cgcgccagaa cacgctccca gcagagaagg
     7261 cttaccacgc agcgcccatt caccgaagat ccagccaaga attgccgccg ccgttgcgac
     7321 cacagtattc acaaatgcca gtgccgcgat ttcattcgcc gtgcccgctg acccggcgtt
     7381 aaagccaaac caaccaatat agagaatggc agtaccagtg aagaccattg gcaggttgtg
     7441 cggtttaaac gcctctttgc cgaagcccac gcgtttgcct atcagatacg cgcccaccag
     7501 accggcgatt gcggcgttaa tatgcaccac cgtgccaccc gcgaaatcca gcgcaccgtg
     7561 agaagccagc aaaccaccgc cccacaccat atgcgcaatc ggaatgtaag agagcgtcag
     7621 ccataccacc acgaaaatca acacagctga gaagcggatt cgttccgcca gcgccccaac
     7681 gatcaagccg acggtaatgc aggcaaacga gccctgaaac gccacgtgga tatactgata
     7741 aatgctgccc atcaccgccg tcagttcgat gttttttagc atcaaccagt taatgttgcc
     7801 gaagaagttg ttgccctcac caaacgccag cgagtaaccg taaaccaccc agagaataca
     7861 gaccagtgca aacgtcaccg tcacctgcgt cagcatcgac agcacgtttt tgccgcgaat
     7921 caacccaccg taaaacaggg caatccccgg aatagtcata aacagcacca gcgcagtaca
     7981 aatcatcata aacgcattgt cggctttatc ggccaccgca ggtgcagcca ttaccagtcc
     8041 cggcagcatc gccagtgaag caagcccagt ttttatcgtc gctatcttca tttttcgttc
     8101 ctgttgctgt gtgccagaga ttacagcgcc gcttcgtcgg cttcgccggt acgaatacga
     8161 atgacgcgtt gcaattcagc gacgaagatt ttgccgtcgc caatttttcc ggtgtaagcc
     8221 gccttactga cgatatcgat cacttcatcg agttggtcat cagcaatcgc cacatcaatt
     8281 tttacttttg gcaggaagtt gacgctgtat tccgcccccc ggtacagctc ggcatgccct
     8341 ttctgacgcc cgaaaccttt cacttcggtg acggtcaggc cctgaatacc aatggaagat
     8401 aacgcttcac gaacgtcttc cagcttgaat ggtttgatta tcacggtcac cagcttcata
     8461 gatcccctcc ggtcagaatt cggtaatggt tctgctacac gaagaagata ttgcaagcgg
     8521 tgtgccagaa atgaaaaatg agaaagagag caggaagtta agaagattga aaatgaaaac
     8581 gcactatgac agtgcacagt gcgttacatt tttgcaccaa cgatgaaata ttgcgctatt
     8641 caggcgctca atgattcctc ttcacgcacg ctggctgcca gctcttcgcc cgcaagttgc
     8701 agttgataca tctgccagta gcgtccctgg gccgccagca gttgctggtg agtgccctgc
     8761 tccacggctt gtccacgatg aagcaccaga atggtgtcgg catcaacaat agttgataag
     8821 cggtgagcaa tcaccaccag cgtggtatgt tcacgcaccg ccgccagagc atgttgaatc
     8881 gcctgttcag taccggagtc aatgctggcg gttgcctcat caaggatcag gatttgcggc
     8941 gtctccacca gcacgcgcgc cagtgccagc agttgctttt gcccgactga gagattattt
     9001 ccctgctcgc ccagcggcgt ataaatacca tcactcatgc tgcgcgccag ctccgccagt
     9061 tgcacggttt ccagcgcctg ccagacgcgt tcttcggaga tatcacgtcc cagcgtcacg
     9121 ttggcgagga aggtatccgc cagcaccacc ggatcttgct gcaccattgc cacgccctgg
     9181 cgcagcgcgc tgtgacttag cgaacttaat ggacgaccat caaggcgaat ctcaccttcc
     9241 gttagcgggt aatagcccat caataaactg gcgagggtgc ttttgccact gccggtatgc
     9301 ccgaccagcg ccacaaagtt acgcgaaggc acagagagat taatgttctt tagcaccaga
     9361 ttgtcatcgc gataagcaaa tgacacgtta tcgacttcga tggtgccgct ctgtaacggg
     9421 cgatcatcat tgccatattg ctggcgcggt ccgtccatca gttcaaacac gcgctcacca
     9481 gcaacaacag cctgttgcag catcgcctgc tgcgtggtca gttcgattaa gggttcgtta
     9541 agtcgcccaa gatagctgat aaacgcatat aacacgccca cttcaatggt gccgctggcg
     9601 gagaagccaa acagcatcaa caagccacaa agaatgagcg atgaaaacag actcagcagc
     9661 ggacgcagca gaaaaccgtc gaggcgcagg gtttgcatcc tcgccatata gtgtgaacga
     9721 ctggcctcac ccatacgttc gccaaatcgc gcctgctgac ggaactgctg gataacgctc
     9781 atgccattga tgatttcgtt aaagccgtcg ttgatatccg ccagataggc gcgcacacga
     9841 cggacaatcg gcgtgctgta acgttgatat atcaccatta ccaccagcac taccgggaaa
     9901 atcattatcg ccaccagcgc cattcgccag tcgaggctga acattgccac cagcatcgcg
     9961 cccaccagtg cggcgctacg caggacagtt gccactacgg taacgtagag atcgcggatc
    10021 acttcagtgt cattagtgac gcgggaaatc acctgcccga cgggttgggt atcaaactcg
    10081 cttaatggct ggcgtaacgc agcatccatc acgtcggtac gcaactgttg cactacgcct
    10141 actgccgccc gattaaacag cagcgactgc gcgtaatgta gcccggcggc aaacagttgt
    10201 agcccaacat acgccgccgc cagccctgca accattttca acggcaggtt atttttcgct
    10261 accatattgt cgataaaata gctgataagc agcggcccac tgacttctgc cgctgccgca
    10321 acccacatca tcaggaccgc aatccccagc ggtttccgcc acggcgaacc gtacgctaac
    10381 agacgcttga gagtcggcca cagttggcta aaactacgca tcgacggcct cctcgcgaat
    10441 ttccggaacg tcgtcgagcg ccgcctccag ttgttgatag cgatacatat cgcgatacca
    10501 gccgctttgt tgcgccagct catcatgatt gccacgctgg gcgatatgtc cgtgctgcat
    10561 cacaataatt tcactggctt ccgtcagtgc cgaaaggcga tgggcactga tgattaccgt
    10621 tcttccctgt ccccactgac gcaggttatg caggatctgg tgctctgtgc gtccgtccac
    10681 cgccgaaagc gcatcatcaa ggatgaggat ttccgcgttg actaataacg ctcgagcaat
    10741 ggagatacgc tgtttttgcc caccggaaag catcacgccg cgctcgccca cttctgtatc
    10801 gtaaccttgc ggcagacgca aaatatcgtc atgtacgctg gctaaccgcg ctacgtgttc
    10861 aatctcttgc tgggtggcat tcgggcaacc cagcgcgatg ttattcgcca cggtgtcaga
    10921 aaaaaggaat ggcgtctggc tgaccaccgc caggcggcta cgccagctat cgagttgtaa
    10981 cttcgtcaga ggaatatcat gaaagcgaat atccccctcg ctgacgtcga aatgacgctg
    11041 aatgagcgac aacagggtac ttttgccgga accagtcggc ccgcaaatac ccagcatctg
    11101 accgggtttc agggcgaaat tgacgttttc cagcgcagga tggtcagtct gcggatacgt
    11161 gaactggtga atatttacat ccagttcgcc acgcccttcc ggcacgggtt cgctaccatc
    11221 gttcaccacc ggcgcttccg ccagcatcgc gcgaatacgg ctgtacgcag cactaccacg
    11281 ttccacaatg ttaaacatcc atgccagcgc cagcattggc caaatcatca gacctaaata
    11341 catcataaaa ctggtgagct ggcccagcgt taaactgccc tgcaccacca tccaactacc
    11401 gccgccaatc gccagcaagt tcgccatacc aatcgcgata tagatggtcg ggtcgaaacg
    11461 agcatcaata cgcgccaccc gcatgttttt tttgccggta tcttcggcat ccgcggcaaa
    11521 taacgccgac tggcgatctt ccagaccaaa ggctttgatc atgcgaatac tggtgaggct
    11581 ttcctgggtg cggtcattaa gactagaaaa cgccgcctgt gccagcttaa agcgttcatg
    11641 caaggcatcg ccgttgcgct taatcatgat cgccatcact ggcatcggta acagggcaaa
    11701 taaggtcaac tgccagctaa tttgcgtaga catcataatc aacacagcgc agcccattac
    11761 cagtgaatcc accagcgtca gcaccccttc tccggcggca aacacgacgc gatcgacgtc
    11821 attagtcgca cgagccatga ggtcacccgt gcgatgacgc aggtaaaact caggatgctg
    11881 ccggcttagc tgacggtaat aatcttcacg cagttcaaca gccagttgat aagacgcacc
    11941 aaacagcaat acccgccaga cgtaacgcag gagataaacc acaacggcaa tcagcaccat
    12001 ggtggcgatc cacatcagga tctgcccggt agtaaagtgt tgttctgtca cgccatcgac
    12061 aacaatacca accacttttg gcggaaccag ttgcagcatc gcgataatga caagcaaggc
    12121 gacagccccg agatagcgac gccattcccg acggaaatac cagcttaatt gagcaaataa
    12181 tcgcacgcgt tgtgtcctga cctgattctg gatatttatt cgatgggtaa agaagtggtg
    12241 tatttaatct gttccatcgc gaagctggaa gtgacgtccg acagccccgg cacgctgttt
    12301 accagacgct tataaaactc gtcgtagcgt ttcatgtcgg caacctggac gcgcatcaga
    12361 taatcgtatt caccagccat gcgccagaac cccagcactt ctggcatttc ggtaaccacc
    12421 gtgacaaagc ggcaatacca ttcgctgctg tgatgttgcg tttttatcag cacaaaggcg
    12481 gtcaggccga ggcctatttt ttccggatcc agcagggcga ctttgccgat aaggataccg
    12541 tcgtcctcca gccgtttcag gcgcttccag caaggggtgg ttgtcagatt aacggcttca
    12601 gccagtgcct gcaaagagag ggtgcaatcc tgctgcagta aggccagcag cttacggtca
    12661 attttatcta acatagccct tccacggaga atttttttct cttttaattc tattttaaag
    12721 gtgaaatcgc gacaatttat tctgtgcggt ttaaacacca cggcacaaaa tgacaaattc
    12781 aacgcaaaaa aaaaccgggc aattgcccgg tgctggaagg gatctcgtta ttcgggggaa
    12841 taaggtagat gtggatagtc cagccagtgc gtcaaatagt gagagacagc ctgatttcgg
    12901 caatgtccaa tcaccggtaa atgcgggagc tccgcgcgca gttgcggcat cgcattgccc
    12961 ataataaagc cgctaccgac gctgcctaac atttcgcgat cgttcatcgc atcaccaaag
    13021 gccatgcaat cgcgcaacga taaacctaaa tgttgggtca gcaccgtcaa tgcagcgcct
    13081 ttattgcagc ccaccggcag cacttcgagg caatccgtgg cggaaaaaca caaatgtgca
    13141 cgctcgccta atgcttcgta tagctggatc tgcaagcgtg taagatcgtc gtgatcgcca
    13201 cagaagcaga tcttggtgac gctgccgagt ggcatttttt tgacatcaat tatctgataa
    13261 cgaaaaccgc tatagacaaa tgcctgcaac aacgcaggga tctctttccc ggtaaaccaa
    13321 ccgtcgtcat tgaagatatg catgctggct cgggtatccc attgctgata cagcaccagc
    13381 tccgcgacat ccgcaggtaa atcatcacga tgtaaaagtt caccttccag agaatgcacg
    13441 cgcgttccgt tgccggtaat caaatatgca tccagcgata gcgccccgag aatatgctgc
    13501 atctccagcg catgacgccc cgtggcaaaa gtgagggtaa tgtcgcgttc acgcagtcgc
    13561 gccaaagtag agagggtttt ctcacctaaa tgatggtcag gcatcaataa agtgccatcc
    13621 atatcaaatg ctgccagacg agccatttct ttctc
//
LOCUS       DAJOWB010000122        13639 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJOWB010000122 DAJOWB010000000
VERSION     DAJOWB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.122"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..186)
                     /locus_tag="OHO85_003863"
     CDS             complement(<1..186)
                     /locus_tag="OHO85_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673176.1"
                     /translation="MSQNDIIIRTHYKSPHRMHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTA"
     gene            709..2637
                     /gene="thrS"
                     /locus_tag="OHO85_003864"
     CDS             709..2637
                     /gene="thrS"
                     /locus_tag="OHO85_003864"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCQ2673177.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2641..3183
                     /gene="infC"
                     /locus_tag="OHO85_003865"
     CDS             2641..3183
                     /gene="infC"
                     /locus_tag="OHO85_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCQ2673178.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3280..3477
                     /gene="rpmI"
                     /locus_tag="OHO85_003866"
     CDS             3280..3477
                     /gene="rpmI"
                     /locus_tag="OHO85_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCQ2673179.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3530..3886
                     /gene="rplT"
                     /locus_tag="OHO85_003867"
     CDS             3530..3886
                     /gene="rplT"
                     /locus_tag="OHO85_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCQ2673180.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4003..4127
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4009..4053
                     /gene="pheM"
                     /locus_tag="OHO85_003868"
     CDS             4009..4053
                     /gene="pheM"
                     /locus_tag="OHO85_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCQ2673181.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4336..5319
                     /gene="pheS"
                     /locus_tag="OHO85_003869"
     CDS             4336..5319
                     /gene="pheS"
                     /locus_tag="OHO85_003869"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCQ2673182.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5334..7721
                     /gene="pheT"
                     /locus_tag="OHO85_003870"
     CDS             5334..7721
                     /gene="pheT"
                     /locus_tag="OHO85_003870"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCQ2673183.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITSEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7726..8025
                     /gene="ihfA"
                     /locus_tag="OHO85_003871"
     CDS             7726..8025
                     /gene="ihfA"
                     /locus_tag="OHO85_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCQ2673184.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8126..9106
                     /gene="btuC"
                     /locus_tag="OHO85_003872"
     CDS             8126..9106
                     /gene="btuC"
                     /locus_tag="OHO85_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCQ2673185.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            9169..9720
                     /gene="btuE"
                     /locus_tag="OHO85_003873"
     CDS             9169..9720
                     /gene="btuE"
                     /locus_tag="OHO85_003873"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCQ2673186.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9720..10469
                     /gene="btuD"
                     /locus_tag="OHO85_003874"
     CDS             9720..10469
                     /gene="btuD"
                     /locus_tag="OHO85_003874"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCQ2673187.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10547..11011
                     /locus_tag="OHO85_003875"
     CDS             10547..11011
                     /locus_tag="OHO85_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HCQ2673188.1"
                     /translation="MRFCLILITALLLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11258..11971
                     /gene="ydiV"
                     /locus_tag="OHO85_003876"
     CDS             11258..11971
                     /gene="ydiV"
                     /locus_tag="OHO85_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCQ2673189.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12034..13470
                     /locus_tag="OHO85_003877"
     CDS             12034..13470
                     /locus_tag="OHO85_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCQ2673190.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGERMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(13474..13593)
                     /gene="hemP"
                     /locus_tag="OHO85_003878"
     CDS             complement(13474..13593)
                     /gene="hemP"
                     /locus_tag="OHO85_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCQ2673191.1"
                     /translation="MRRISSQTLLGPDGKLIIDHDGQEYLLRKTQAGKLLLTK"
BASE COUNT         3402 a         3185 c         3669 g         3383 t
ORIGIN      
        1 tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa cttaagtcag aggtatcaag
       61 tagggtgatg agttggcgcg caaattgatt aataggctct gatgaaggcg ttggtatgtc
      121 gctatcgatg tgcattctat gaggagactt ataatgagtt ctgataataa tgtcgttttg
      181 actcataatc tattcgcctt atatatgtct ggaggttttg agtatgatga ttaattatta
      241 tcgccagaac gaaatgtgca cgaaaaattg ctataagaat tgtgtcactt ttatcattgt
      301 gatatgacca gttcgtcatt aatcttatga tcgtttaaat actaccgcca aatcatgcaa
      361 cgaactttgc tggattcagt ttatgctgct gtaaatccgc tcgcagtaaa cctttcagac
      421 gctccgtgat gttatcagtt gttctttaag cgttttgctg gtgtactcac tacaaacgaa
      481 ttgcgaatcg aatcaatgtg aaacggaaag gtacaatctc ccttttttta gtgttaacat
      541 cgctcaaccg ggtggaaggt gagaggttaa tgtctttcat ttgtgtacct gattgcgaac
      601 caatttagca tttgttggct agatggtttc gcaatgaact gttaataaac aaatttttct
      661 ttgtatgtga tctttcgtgt gggtcaccac tgcaaataag gatataaaat gcctgttata
      721 actcttcctg atggcagcca acgccattac gaccacgctg taagccccat ggatgttgcg
      781 ctggacattg gtccaggtct ggcgaaagcc tgtatcgcag ggcgcgttaa tggcgaactg
      841 gttgatgctt gcgatctgat tgaaaacgac gcacaactgt cgatcattac cgccaaagac
      901 gaagaaggtc tggagatcat tcgtcactcc tgtgcgcacc tgttagggca cgcgattaaa
      961 caactttggc cgcataccaa aatggcaatc ggcccggtta ttgacaacgg tttttattac
     1021 gacgttgatc ttgaccgcac gttaacccag gaagatgtcg aagcactcga gaagcggatg
     1081 catgagcttg ctgagaaaaa ctacgacgtc attaagaaga aagtcagctg gcacgaagcg
     1141 cgtgaaactt tcgccaaccg tggggagagc tacaaagtct ccattcttga cgaaaacatc
     1201 gcccatgatg acaagccagg tctgtacttc catgaagaat atgtcgatat gtgccgcggt
     1261 ccgcacgtac cgaacatgcg tttctgccat catttcaaac taatgaaaac ggcaggggct
     1321 tactggcgtg gcgacagcaa caacaaaatg ttgcaacgta tttacggtac ggcgtgggca
     1381 gacaaaaaag cacttaacgc ttacctgcag cgcctggaag aagccgcgaa acgcgaccac
     1441 cgtaaaatcg gtaaacaact cgacctgtat catatgcagg aagaagcgcc gggtatggta
     1501 ttctggcaca acgacggctg gaccattttc cgtgaactgg aagtgtttgt tcgttctaaa
     1561 ctgaaagagt accagtatca ggaagttaaa ggtccgttca tgatggaccg tgtcctgtgg
     1621 gaaaaaaccg gtcactggga caactacaaa gatgcaatgt tcaccacgtc ttctgagaac
     1681 cgtgaatact gcattaagcc gatgaactgc ccgggtcacg tacaaatttt caaccagggg
     1741 ctgaagtctt atcgcgatct gccgctgcgt atggccgagt ttggtagctg ccaccgtaat
     1801 gagccgtcag gttcgctgca tggcctgatg cgcgtgcgtg gatttaccca ggatgacgcg
     1861 catatcttct gtactgaaga acaaattcgc gatgaagtta acggatgtat ccgtttagtc
     1921 tatgatatgt acagcacttt tggcttcgag aagatcgtcg tcaaactctc cactcgtcct
     1981 gaaaaacgta ttggcagcga cgaaatgtgg gatcgtgctg aggcggacct ggcggttgcg
     2041 ctggaagaaa acaacatccc gtttgaatat caactgggtg aaggcgcttt ctacggtccg
     2101 aaaattgaat ttaccctgta tgactgcctc gatcgtgcat ggcagtgcgg tacagtacag
     2161 ctggacttct ccttgccgtc tcgtctgagc gcctcctatg tgggtgaaga caacgagcgt
     2221 aaggtaccgg taatgattca ccgcgcaatt cttgggtcga tggaacgttt catcggtatc
     2281 ctgaccgaag aattcgctgg tttcttcccg acctggcttg cgccggttca ggttgttatc
     2341 atgaatatta ccgattcaca gtctgattac gttaacgaat tgacgcaaaa actatcaaat
     2401 gcgggcattc gtgttaaagc agacttgaga aatgagaaga ttggctttaa aatccgcgag
     2461 cacactttgc gtcgcgtccc atatatgctg gtctgtggtg ataaagaggt ggaatcaggc
     2521 aaagttgccg ttcgcacccg ccgtggtaaa gacctgggaa gcatggacgt aaatgaagtg
     2581 atcgagaagc tgcaacaaga gattcgcagc cgcagtctta aacaattgga ggaataaggt
     2641 attaaaggcg gaaaacgagt tcaaacggcg cgccctaacc gtatcaatgg cgaaattcgc
     2701 gcccaggaag ttcgcttaac aggtctggaa ggcgagcagc ttggtattgt gagtctgaga
     2761 gaagctctgg agaaagcaga agaagccgga gtagacttag tcgagatcag ccctaacgcc
     2821 gagccgccgg tttgtcgtat aatggattac ggcaaattcc tctatgaaaa gagcaagtct
     2881 tctaaggaac agaagaaaaa gcaaaaagtt atccaggtta aggaaattaa attccgtcct
     2941 ggtacagatg aaggcgacta tcaggtaaaa ctccgcagcc tgattcgctt tctcgaagag
     3001 ggtgataaag ccaaaatcac gctgcgtttc cgcggtcgtg agatggcgca ccagcaaatc
     3061 ggtatggaag tgcttaatcg cgtgaaagac gatttgcaag aactggcggt ggtcgaatcc
     3121 ttcccaacga agatcgaagg ccgccagatg atcatggtgc tcgctcctaa gaagaaacag
     3181 taaggccttc aagtagcact gtctgtggag ccttcgggct tcacaggcat tgttcgcctt
     3241 tgtttcgttt attaacaatg cgaagtggaa gttattaaaa tgccaaaaat taagaccgta
     3301 cgcggtgctg ctaagcgctt caaaaaaacc ggtaaaggtg gttttaagca caagcacgct
     3361 aacctgcgtc acattctgac caaaaaagcg accaaacgta aacgtcacct gcgtccgaaa
     3421 gccatggttt ccaaaggcga tctgggcctg gtaatcgcgt gcctgccgta cgcataagcc
     3481 gttaacgttt ttaacttttt aattagaata tagatacagg agagcacata tggctcgcgt
     3541 aaaacgtggt gttattgcac gcgcacgtca caagaaaatt ttgaaacaag ctaaaggcta
     3601 ctacggtgcg cgttctcgcg tataccgcgt tgccttccag gctgttatca aagctggtca
     3661 gtatgcttac cgtgaccgtc gtcaacgtaa gcgtcagttc cgtcaactgt ggattgcgcg
     3721 tatcaacgca gcagcacgtc agaacggtat ttcttacagc aaattcatca atggcctgaa
     3781 aaaagcctct gttgaaatcg accgtaagat cctggctgat atcgcagtat tcgacaaagt
     3841 agcgttcacc gctctggttg aaaaagcgaa agcagctctg gcataagcca gttgaaagag
     3901 ggagctagtc tccctctttt cgtttcaacg ccatcaaaac attgactttt atcgccgtag
     3961 ccttttcaat aaaggtcttt tgaagagtac caaaaggtaa cgcaagcaat gaatgctgct
     4021 attttccgct tcttttttta ctttagcacc tgaatccagg aggctagcgc gtgagaagag
     4081 aaacggaaaa cagcgcctga aagcctccca gtggaggctt tttttgtatg cgcgttttga
     4141 aaaataaaaa gccagtctat caggcgtaat tgtcgcagcc agtctggaca cggccagcgc
     4201 ggagaaaccg gagcgtaatt aagtacgtga ggatttcgag cactgcccgg gaccaaaatg
     4261 gcaagtaaaa tagcctgatg ggataggctc taagtccaac gaaccagtgt caccactgac
     4321 acaatgagga aaaccatgtc acatctcgca gaactggttg ccagtgcgaa ggcggccatt
     4381 agccaggcgt cagatgttgc cgcgttagat aatgtgcgcg tcgaatattt gggtaaaaaa
     4441 gggcacttaa cccttcagat gacgaccctg cgtgagctgc cgccagaaga gcgtccggca
     4501 gctggtgcgg ttatcaacga agcgaaagag caggttcagc aggcgctgaa tgcgcgtaaa
     4561 gcggaactgg aaagcgctgc actgaatgcg cgtctggcgg cggaaacgat tgatgtctct
     4621 ctgccaggtc gtcgcattga aaacggcggt ctgcatccgg ttacccgtac catcgaccgt
     4681 atcgaaagtt tcttcggtga gcttggcttt accgtggcaa ccgggccgga aatcgaagac
     4741 gattatcata acttcgatgc tctgaacatt cctggtcacc acccggcgcg cgctgaccac
     4801 gacactttct ggtttgacgc tacccgcctg ctgcgtaccc agacctctgg cgtacagatc
     4861 cgcaccatga aagcccagca gccaccgatt cgtatcatcg cgcctggccg tgtttatcgt
     4921 aacgactacg accagactca cacgccgatg ttccatcaga tggaaggtct gattgttgat
     4981 accaacatca gctttaccaa cctgaaaggc acgctgcacg acttcctgcg taacttcttt
     5041 gaggaagatt tgcagattcg cttccgtcct tcctacttcc cgtttaccga accttctgca
     5101 gaagtggatg tcatgggtaa aaacggtaaa tggctggaag tgctgggctg cgggatggtg
     5161 catccgaacg tgctgcgtaa cgttggcatc gacccggaag tttactctgg tttcgccttc
     5221 gggatgggga tggagcgtct gactatgttg cgttacggcg tcaccgacct gcgttcattc
     5281 ttcgaaaacg atctgcgttt cctcaaacag tttaaataag gcaggaatag attatgaaat
     5341 tcagtgaact gtggttacgc gaatgggtga acccggcgat tgatagcgat gcgctggcaa
     5401 atcaaatcac tatggcgggc ctggaagttg acggtgtaga accggttgcc ggtagcttcc
     5461 acggcgtggt agttggtgaa gtggttgagt gtgcgcagca tccgaacgct gacaaactgc
     5521 gtgtgacaaa agtgaatgtt ggcggcgatc gcctgctgga catcgtctgc ggtgcgccaa
     5581 actgccgtca gggcctgcgt gtggcggtag cgaccattgg tgctgttctg ccgggtgatt
     5641 tcaaaattaa agcggcgaaa ctgcgtggtg aaccgtctga agggatgctg tgctcctttt
     5701 ctgagctggg aatttctgac gatcatagcg gcattatcga gctgcctgcg gatgcgccga
     5761 ttggcaccga catccgtgaa tacctgaaac ttgatgacaa caccatcgaa atcagcgtga
     5821 cgccaaaccg tgccgactgc ttaggcatca ttggtgttgc gcgtgacgtt gccgtgctga
     5881 accagctgcc gctggttgaa ccggaaatcg ttccggttgg tgcgaccatc gacgacacgc
     5941 tgccgattac agtcgaagcg ccggaagcct gcccgcgtta tcttggccgt gtggtaaaag
     6001 gcattaacgt taaagcgcca actccgctgt ggatgaaaga aaaactgcgt cgttgcggga
     6061 tccgttctat cgatgcagtt gttgacgtca ccaactatgt gctgctcgaa ctgggccagc
     6121 cgatgcacgc tttcgataaa gatcgcattg aaggcggcat tgtggtgcgg atggcaaaag
     6181 agggcgaaac gctggtgctg cttgatggca ctgaagcgaa gctgaatgct gacactctgg
     6241 tcatcgccga ccacaacaag gcgctggcga tgggcggcat ctttggtggc gaacactctg
     6301 gcgtgaatga cgaaacgcaa aacgtcctgc tggaatgcgc tttcttcagc ccgctgtcta
     6361 tcaccggtcg tgctcgtcgt catggcctgc atactgatgc gtctcaccgt tatgagcgtg
     6421 gcgttgatcc ggcactgcag cacaaagcga tggaacgtgc gacccgtctg ctgatcgaca
     6481 tctgcggtgg tgaggctggt ccggtaattg atatcaccag cgaagcaacg ctgccgaagc
     6541 gtgcaaccat cactttacgt cgtagcaaac tggatcgcct gatcggccat catattgcgg
     6601 atgagcaggt aactgacatt ctgcgtcgtc tcggctgcga agtgaccgaa ggcaaagacg
     6661 agtggcaggc agttgcgccg agctggcgtt tcgatatgga gattgaagaa gatctggtcg
     6721 aagaagtcgc gcgtgtttac ggctacaaca acatcccgga tgagccggta caggcaagcc
     6781 tgattatggg tactcaccgt gaagctgacc tgtcgctcaa gcgcgtgaaa acgctgctca
     6841 acgacaaagg ctatcaggaa gtgatcacct acagcttcgt tgatccgaaa gtgcagcaga
     6901 tgatccatcc aggcgttgaa gccttactgc tgccaagccc gatctctgtt gaaatgtcag
     6961 caatgcgtct ttctctgtgg accggcctgc tggcaaccgt ggtgtacaac cagaaccgtc
     7021 agcagaaccg tgtgcgcatt ttcgaaagcg gtctgcgttt cgtaccagat actcaagcac
     7081 cgttgggcat tcgtcaggat ctgatgttag ccggtgtgat ttgcggtaac cgttacgaag
     7141 agcactggaa cctggcaaaa gagaccgttg atttctatga tttgaaaggc gatcttgaat
     7201 ccgttctcga cctgaccggt aaactgaatg aggttgagtt ccgtgcagaa gcgaatccgg
     7261 cactgcatcc ggggcaatcc gcagcgattt atctgaaagg tgaacgtatt ggttttgttg
     7321 gggttgttca tcctgaactg gaacgtaaac tggatcttaa cggtcgcact ctggtgttcg
     7381 aactggagtg gaacaagctc gcagaccgcg tggtgcctca ggcgcgcgag atttctcgct
     7441 tcccggcgaa ccgtcgtgac atcgcggtgg tggtcgcaga aaacgttccc gcagcggata
     7501 ttttatccga atgtaagaaa gttggcgtaa atcaggtagt tggcgtaaac ttatttgacg
     7561 tgtaccgcgg taagggtgtt gcggaggggt ataagagcct cgccataagc ctgatcctgc
     7621 aagataccag ccgtacactc gaagaagagg agattgccgc taccgtcgcc aaatgtgtag
     7681 aggcattaaa agagcgattc caggcatcat tgagggattg aacctatggc gcttacaaaa
     7741 gctgaaatgt cagaatatct gtttgataag cttgggctta gcaagcggga tgccaaagaa
     7801 ctggttgaac tgtttttcga agagatccgt cgcgctctgg aaaacggcga acaggtgaaa
     7861 ctctctggtt ttggtaactt cgatctgcgt gataagaatc aacgcccggg acgtaacccg
     7921 aaaacgggcg aggatattcc cattacagca cggcgcgtgg tgaccttcag acccgggcag
     7981 aagttaaaaa gccgggtcga aaacgcttcg cccaaagacg agtaatctga tctaactaaa
     8041 aaggccgctc tgcggccttt tttcttttca ctgtcgaaga gtcaccgtaa aatcaacgcc
     8101 atgacacttc agcagaacgg ataccatgct gacacttgcc cgccaacaac agcgacaaaa
     8161 tattcgctgg ttattatgcc tgtcagtttt gatgctgctg gcgcttctct taagcctttg
     8221 cgccggtgaa caatggatct cgccaggtga ctggtttact cctcgtggcg aactgttcgt
     8281 ctggcaaatt cgcctgccac gtacgctggc tgtattgctg gttggtgcgg cgctggctat
     8341 atccggcgct gtaatgcagg cgttgtttga aaatcctctg gcagaacctg gactacttgg
     8401 cgtctctaac ggcgcaggcg tggggcttat cgccgcggta ttgcttgggc aagggcaact
     8461 ccccaactgg gcgctagggc tgtgtgcgat tgctggcgcg cttatcatca ctttaatact
     8521 cttacgtttc gcccgtcgtc atctttcgac cagtcggtta ttgctggctg gcgttgcatt
     8581 agggattatc tgtagcgcac taatgacgtg ggctatctac ttttccacct cagttgattt
     8641 gcgtcagctg atgtactgga tgatgggcgg ttttggcggc gtagactggc ggcaaagctg
     8701 gctgatgctg gcattgatcc ccgtgttgtt gtggatctgt tgtcagtcca ggccgatgaa
     8761 tatgttagca cttggcgaga tctcggcgcg gcaactgggt ttacccctgt ggttctggcg
     8821 caatgtgctg gtcgcagcga ccggctggat ggttggcgtc agtgtggcgc tggcgggtgc
     8881 tatcggcttt attggtctgg tgatcccaca tattctccgg ctgtgtggtt taaccgatca
     8941 tcgcgtatta cttcccggct gcgcgctggc aggggcgagc gcattgctgc tggccgatat
     9001 tgtagcgcgc ctggcattag ctgccgcaga gctgcctatt ggcgtggtca ccgcaacgtt
     9061 gggtgcgccg gtgtttatct ggttattgtt aaaagcaaga cgttagccgc aaaaagacgg
     9121 tctatgatta aaagctaaga tttttactga ccacacccag gagaaacgat gcaagattcc
     9181 attctgacga ccgtagtgaa agatatcgac ggtgaagtga ccacgctgga gaagtacgcc
     9241 ggtaatgtgc tgttgattgt caatgtcgcc tcaaagtgtg gcttaacgcc gcaatatgag
     9301 cagttggaga atattcagaa agcctgggcc gatcgaggtt ttgtggtgct gggattcccg
     9361 tgcaaccagt ttctggaaca agaaccgggc agcgatgaag agattaaaac ttactgtacc
     9421 accacatggg gggtgacgtt cccgatgttc agtaagattg aagttaatgg cgaaggacgc
     9481 catccgctgt atcaaaaatt gattgccgca gcgccgaccg cagtcgcgcc ggaagagagc
     9541 ggattctatg cccgtatggt cagcaaaggc cgtgcaccgc tgtacccgga tgatatttta
     9601 tggaattttg aaaaattcct ggttggcagg gacggaaaag tcatccagcg tttttccccg
     9661 gatatgacgc cggaagatcc cattgtgatg gaaagcatta aactggcgtt ggcaaaataa
     9721 tgtctattgt gatgcagtta caagatgttg cggaatctac ccgcctgggg ccgctttctg
     9781 gcgaggttcg ggctggggag atcctgcacc tggtggggcc gaatggcgcg ggtaagagta
     9841 ccttactggc gcgaatggcc ggaatgacca gcggtaaggg aagcattcag ttcgcggggc
     9901 aaccactgga agcatggtcc gcaacaaaac tcgcgctgca tcgcgcctat ctttcacaac
     9961 agcagacgcc gccgtttgca atgccggtct ggcactacct gacactgcat cagcacgata
    10021 aaacgcgtac cgaactactg aatgatgtcg caggggcgct ggctcttgat gacaaactcg
    10081 gacgtagcac caatcaactt tccggcggtg aatggcaacg cgtacgtctt gctgcggtgg
    10141 tgttgcaaat cacaccacaa gccaatcccg caggccaatt gctgcttctt gatgagccga
    10201 tgaacagtct tgatgttgcg caacaaagtg cgttagacaa aattctgagc gcgctgtgtc
    10261 agcaaggact ggcgattgtg atgagcagtc acgatctcaa ccacacattg cgtcatgcgc
    10321 atcgggcgtg gttgctaaaa ggtggaaaaa tgctggccag tggacgcagg gaagaggtgc
    10381 tcacgccgcc aaatctggcg caggcctatg ggatgaattt tcgccgtctg gatatcgaag
    10441 gtcacagaat gctgatttcg accatctgat ttcattagta tcttgaaaat cgggtaatta
    10501 cgacgctaaa ttaatgcaag aataaaaaac agaggaaccg tacggaatgc gtttctgcct
    10561 tattttgatc acagcactgc ttctggccgg gtgtagccac cataaagcac cgccgccaaa
    10621 tgccagactt tctgattcga ttaccgttat tgccggtttg aacgaccagc tacaaagctg
    10681 gcatggcacg ccgtatcgtt atggtggcat gacgcggcgc ggtgtggact gttcgggatt
    10741 tgtggttgtg acgatgcgcg atcgtttcga tttgcagctg ccccgtgaaa ccaaacaaca
    10801 agcctctatt ggcacgcaaa ttgataaaga cgagttgctg ccaggtgacc tggtcttttt
    10861 caaaacgggg tccggacaaa atggtttgca tgtaggtatt tacgatacca acaaccaatt
    10921 tatccacgcc tctaccagca agggagtgat gcgttcctca cttgataatg tctattggca
    10981 gaaaaatttc tggcaggcga gacgaatcta gtatgtcgaa aaatggacgg cgatgataca
    11041 tcgccgtcca tttactcctg aatacagtag tattttgcag acaaaaattc cgtaaatgga
    11101 ttattcgttt ataatcagga taatctaaaa aaaggacccc tgaaaaagtg gttaacatgg
    11161 aacaaatgaa agttactcct tctgtaatta ttatgtctta tggcctcatc ccttttagcc
    11221 ggatactgaa aaacatcctt cgagagggac ggttaccatg aagatttttt tggagaatct
    11281 ttatcattct gattgctatt tcctgccgat aagagacaac cagcaagtcc ttgtcggcgt
    11341 tgagttaatt acccatttct ccagtgaaga tggcacggtg cgtatcccga cgagtcgggt
    11401 tatagcgcaa cttaccgagg agcaacattg gcaattattt tcagagcagt tggaattact
    11461 aaaatcatgc caacattttt ttattcaaca taaactcttt gcctggctta atttaacgcc
    11521 acaagtggct acattattat tagagaggga taattacgca ggcgaactat taaaatatcc
    11581 gtttattgaa ctgttgatta atgagaatta cccgcatctc aatgaaggaa aagataatcg
    11641 cggtctttta tccttatcgc aagtgtatcc ccttgtgtta ggtaatttgg gagcaggcaa
    11701 tagcacaatg aaagcggttt ttgatggtct tttcacccgc gtcatgctgg ataaaagttt
    11761 tattcagcaa caaatcacgc accgctcctt cgaaccgttt attcgtgcga tccaggcgca
    11821 aatttcccct tgttgcaatt gcatcattgc gggaggcatt gatacggcag aaatattggc
    11881 acagattacc cccttcgatt tccatgctct ccagggatgc ctgtggcctg ccgttcccat
    11941 aaatcagata acgacgttgg ttcagcgata acccttctgt ttgctggtgt ttaagacgag
    12001 agtaaccgtc tacactatca aacaggagga tctatgaccc tgtcttttgt tacccgctgg
    12061 cgcgatgaat tgccagaaac ctatacagca ctttccccta cgcctttaaa taatgcccgg
    12121 ctgatttggc ataataccga actggctaac acgctgagta ttccatcgtc gctgtttaaa
    12181 aatggcgcag gtgtctgggg cggcgaagcc ctactgcctg gtatgtcacc actggcgcag
    12241 gtttacagtg gtcatcagtt cggcgtctgg gcgggccaac tgggtgatgg gcgcggcatt
    12301 ttactcggcg aacaactgct tgctgatggc actacaatgg actggcatct gaaaggtgct
    12361 ggcctgacgc cttattcgcg aatgggtgat ggacgggcgg ttttacgttc gacgatacga
    12421 gaaagtctcg ccagtgaggc gatgcattat ctgggcattc cgacgacccg cgcgttaagt
    12481 atcgtcacca gcgattcgcc agtgtatcgg gaaacggcgg agccaggcgc gatgctgatg
    12541 cgtgtggcac caagtcatct gcgctttggt catttcgaac atttttacta ccgccgcgag
    12601 tcggaaaagg ttcgtcagtt agctgacttt gctattcgtc attactggtc acatcttgaa
    12661 gatgatgagg acaaataccg tctctggttt agcgatgttg tcgcacgtac cgcgtcgtta
    12721 attgcccaat ggcagacggt cggctttgct catggggtga tgaataccga caacatgtcg
    12781 ctgctggggc tgacgcttga ttacgggccg tttggttttc ttgatgatta cgaacccggt
    12841 tttatttgta atcactcgga tcatcaaggg cgttacagct ttgataatca acctgccgtc
    12901 gcgttgtgga atttacagcg tctggcgcag acattgtcgc catttgttgc cgtagatgcc
    12961 ctgaatgagg ccctggacag ctatcagcag gttttgttga cgcattatgg agaacggatg
    13021 cggcagaaac tgggcttcat gacggagcaa aaagaggata acgcgctact gaatgaatta
    13081 ttcagtctga tggcgcgaga gcgcagcgat tatacccgca cattccgcat gctgagtctg
    13141 accgagcagc acagcgcggc gtcaccgcta cgtgatgagt ttattgatcg tgcggcattt
    13201 gatgactggt ttgcccgtta tcgggggcgt ttgcaacaag acgaggttag cgatagtgag
    13261 cgtcagcagc tgatgcaaag cgttaaccca gctctggtgt tgcgcaactg gttggcgcaa
    13321 cgggcgattg aagcggcaga aaagggtgat atgacggaat tgcaccgcct gcatgaggcg
    13381 ttgcgaaatc ctttcagcga cagagctgat gactatgtca gtcgtccacc tgactggggt
    13441 aaacggctgg aagtcagctg ctcgagttaa aggctacttg gtcaacagca gcttgccagc
    13501 ctgagtttta cggagcagat attcttgccc gtcatgatcg ataatcagtt tgccatccgg
    13561 acctaacagt gtctggctgg aaattcgccg aataggctgc ggggaagcgg tcttgtgatt
    13621 ggcatccgtt tcaggcgtg
//
LOCUS       DAJOWB010000123        13408 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJOWB010000123 DAJOWB010000000
VERSION     DAJOWB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.123"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            90..785
                     /locus_tag="OHO85_003879"
     CDS             90..785
                     /locus_tag="OHO85_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCQ2673192.1"
                     /translation="MLSCLSPTLVCAEQPHGFIDYRHEYLDDTRTHADRVEFGTFFSN
                     GIGLMGELRYNTDEGDKDKWDPSQFGNNGTGLSVVYRFKPLDDKKFWLEPMFWLDTTQ
                     YWSTYEYGLSAGYDFSKEWKVSGRFRYDMDKATDKSKGYGNDDRNNRRYDVWIDYRPQ
                     KTNFQYQLNLVYYNNGYLTWNDGHKNYTASFKVGYKIGSWIPYMSIADYKGVDKTSSN
                     RQIRWRWGLTYTF"
     gene            798..1667
                     /locus_tag="OHO85_003880"
                     /pseudo
     CDS             798..1667
                     /locus_tag="OHO85_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060172.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2608 domain-containing protein"
     gene            complement(1712..3604)
                     /gene="parE"
                     /locus_tag="OHO85_003881"
     CDS             complement(1712..3604)
                     /gene="parE"
                     /locus_tag="OHO85_003881"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCQ2673193.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3633..4214)
                     /gene="yqiA"
                     /locus_tag="OHO85_003882"
     CDS             complement(3633..4214)
                     /gene="yqiA"
                     /locus_tag="OHO85_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCQ2673194.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4214..5041)
                     /gene="cpdA"
                     /locus_tag="OHO85_003883"
     CDS             complement(4214..5041)
                     /gene="cpdA"
                     /locus_tag="OHO85_003883"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCQ2673195.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCRWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(5066..5488)
                     /locus_tag="OHO85_003884"
     CDS             complement(5066..5488)
                     /locus_tag="OHO85_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCQ2673196.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5489..6118)
                     /gene="nudF"
                     /locus_tag="OHO85_003885"
     CDS             complement(5489..6118)
                     /gene="nudF"
                     /locus_tag="OHO85_003885"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCQ2673197.1"
                     /translation="MLKPDNLPVTLGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6323..7804
                     /gene="tolC"
                     /locus_tag="OHO85_003886"
     CDS             6323..7804
                     /gene="tolC"
                     /locus_tag="OHO85_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCQ2673198.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7952..8623
                     /locus_tag="OHO85_003887"
     CDS             7952..8623
                     /locus_tag="OHO85_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCQ2673199.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8629..9789
                     /locus_tag="OHO85_003888"
     CDS             8629..9789
                     /locus_tag="OHO85_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCQ2673200.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9827..10615)
                     /gene="ygiD"
                     /locus_tag="OHO85_003889"
     CDS             complement(9827..10615)
                     /gene="ygiD"
                     /locus_tag="OHO85_003889"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCQ2673201.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGTL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVEGIEMGSLSMLSVQVG"
     gene            10758..11531
                     /gene="zupT"
                     /locus_tag="OHO85_003890"
     CDS             10758..11531
                     /gene="zupT"
                     /locus_tag="OHO85_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCQ2673202.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11589..11759)
                     /locus_tag="OHO85_003891"
     CDS             complement(11589..11759)
                     /locus_tag="OHO85_003891"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673203.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(12020..12673)
                     /gene="ribB"
                     /locus_tag="OHO85_003892"
     CDS             complement(12020..12673)
                     /gene="ribB"
                     /locus_tag="OHO85_003892"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCQ2673204.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12777..12925)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            13047..13337
                     /gene="ubiK"
                     /locus_tag="OHO85_003893"
     CDS             13047..13337
                     /gene="ubiK"
                     /locus_tag="OHO85_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCQ2673205.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3461 a         3491 c         3222 g         3234 t
ORIGIN      
        1 gatagtgtat tcgaccacca taaatatatt ttattctgaa agggaatatt atgaattctt
       61 atcttaaaat atcacttgca ggaataacaa tgttatcctg cctgtcacct acactagtct
      121 gtgcagaaca gccgcatgga tttattgatt atcgtcatga gtatcttgat gatactcgca
      181 cccacgcgga ccgcgttgaa tttggtacat tttttagtaa tggtatcggt ttgatgggag
      241 aattacgcta taacaccgat gaaggtgata aagataagtg ggacccctca caatttggca
      301 ataacggaac aggattatct gttgtttacc gctttaagcc gctggatgac aaaaaattct
      361 ggttagaacc catgttctgg cttgatacca cgcaatattg gtcaacttac gaatatggtc
      421 tttctgcggg ttatgacttc agcaaagaat ggaaagtttc gggtcggttt cgttatgaca
      481 tggataaagc caccgataaa tccaaaggtt atggcaacga tgatcgtaat aaccgccgtt
      541 acgatgtgtg gatcgactat cgaccacaaa aaaccaactt ccaatatcag ttaaacctgg
      601 tctattacaa taatggttat ttaacctgga atgatggcca taaaaactat accgcatcat
      661 ttaaagtagg gtacaaaatt ggatcgtgga taccctatat gagtattgcg gattataaag
      721 gtgtggataa aacatcaagt aatcgccaaa tccgctggcg ctggggtctg acttatactt
      781 tctaatctgg aataaaaatg aataataaat ttaaaatcat tctctggatg atgctctctg
      841 ctctgcctgc tttatcttac gcataggtaa aaaatatagt tactggcgaa tatagtgcgg
      901 tcagtaaaat tatcaccgaa aaggaaactc tctatccatc cgataagata ttattggttt
      961 ttgacattga taatacgtta ttaacgagtg gcacacgcat tggcggtgat atctggtatc
     1021 aatggcaaac cgataaattg cctttaaagc cggatgactc gcaaaaagtt ccttgcctgt
     1081 atgaaaatac aatcagtatg ctttacgaac ttagccctat gcagcttacc gagccacagt
     1141 taccgacaat gttaaaacag tggcaacaaa agcataccgc ttttgctctg acatcccgtg
     1201 caccggatac tctctttccg acattgcggg aattaaagcg aaacggcatt gatttttcgt
     1261 cttccgcact caggaagaaa gaggatactt tgccaccctt tgaaaaaggg aaactcaaac
     1321 ggtcatggct gtacagccag ggggtatttt tttcttccgg gcaagataaa ggcgtaattc
     1381 tcgattatat gctggataaa atggggaaca aatatgacgc gattgttttt atcgatgacg
     1441 ggcaagcaaa cattcatgcg atgacgaaga tgttctctaa ggataaatgg ttctctacgg
     1501 attttaccat tattcactat accagagtgg aggatgagct tattaaacaa caaggtgcag
     1561 taataacaac tgcacaagca gaaaaaatgg ctacggactg gaaaactctg gctggttcgc
     1621 tggcaacact atttccagat cgtaacaaac tgtgcccaat aaaataatca acgacaaccc
     1681 ggcaaaacac atgtttgccg ggttaagatt atcagacctc aatctccgcc atgtcgcctt
     1741 tctcttgcaa ccagttgcgg cgatcttccg agcgtttctt cgccagtagc atgtccatca
     1801 tcgcgtcagt acgctgatcg tcttcatcat cgatagtcaa ctgcaccaga cggcgagtgt
     1861 tcggatcaag cgtggtttca cgcaattgca tcgggttcat ttcccccaga cctttaaaac
     1921 gctggacgtt cggcttgcct ttcttgcgtt ttaattgctc aagtacgccc tctttctctt
     1981 cttccgtcag cgcgtaataa acctctttcc cgagatcaat acggtagagc ggtggcagtg
     2041 cgacgtaaac gtgaccgtgt ttcaccaacg cgcggaagtg ttttacgaac aaagcgcaga
     2101 gtagcgtggc aatgtgcaga ccatcagagt ccgcatccgc gaggatacag attttgccat
     2161 aacgaagctg gctcagatcg tcgctgtcag gatcgatacc gatcgctacc gaaatatcat
     2221 gcacttcctg cgaggccagc acttcgtcgg aagaaacttc ccaggtgtta aggatcttac
     2281 ctttcagcgg catgatcgcc tgatattcac gatcgcgcgc ctgcttggca gatccgcctg
     2341 cggagtcacc ttccacgagg aacagttcgg tacggttaag gtcctgcgcg gtacaatcag
     2401 ccagtttgcc aggcagcgcc gggccgctgg tcagcttttt acgcaccact tttttagccg
     2461 cacgcatacg acgctgggcg ctggaaatcg ccatctctgc cagcaactcc gccgcctgaa
     2521 cgttctggtt cagccacagg ataaaggcat ctttcaccac gccagaaacg aatgccgcgc
     2581 attgacgcga agagagacgc tctttcgtct gcccggcaaa ctgcggatcc tgcattttca
     2641 ccgacagaac ataggcgcag cgatcccaga tatcttccgc cgacagcttc acgccgcgcg
     2701 gcagaatgtt gcgatattca cagaactcac gcatcgcgtc caacaggccc tgacgcaggc
     2761 cattaacatg ggtaccgccc tgcatcgttg ggatcaggtt gacgtagctt tcggtcagca
     2821 gttcaccgcc ttccggcagc cacagtagcg cccagtccac agcttcagta tcgccagcga
     2881 aattaccgat aaacggtttt tctggcagcg tcggtaaacc gtttaccgct tccgccaggt
     2941 aatcattcag accgtcctga tagcaccagc gctgttcggt gttgttgatc tcatctttaa
     3001 aggtaatctc aacgccgggg cacagtaccg ctttggcttt cagcacatgc gtcaggcgtg
     3061 aaacagaaaa tcgcgggctg tcaaagaagg tttcatccgg ccagaagtgc acgctggtac
     3121 cggtattgcg tttaccgcaa gtgccgacaa cctgtaaatc ctgcaccttt tcgccatttt
     3181 caaaggcgat gttataaacc tgaccatcgc ggcgcacgtt aacttcaacg cgcttcgaca
     3241 gggcgttaac caccgaaatc cccacgccat gcaggccgcc agagaactgg taatttttgt
     3301 tagagaattt accgcctgca tgcagacggc aaagaatcaa ttcaaccgcc ggtacaccct
     3361 cttccgggtg aatatccacc ggcatcccgc gcccatcgtc aataacttct aacgactggt
     3421 cagcatgtaa gattacatcc acgcgttttg cgtgacccgc cagtgcttca tcgacactgt
     3481 tatcaataac ttcttgcccc aaatggttag ggcgagtggt atcggtatac atccccggac
     3541 ggcggcgaac cggctcaagc ccggtgagta cctcaatggc atcagcgtta taagtttgcg
     3601 tcatggttta agttagtaat tcgagttgat cgtcagagat ggtgcagacc aagaaaatcg
     3661 acgatcgggt tgaaataatc ttcgaagccc gtgaatgcgt ggttgccgcc ttctatgaca
     3721 gtctggcggc aggaagcgta gtacgccacc gcctggcggt aatccagcac ttcatctccc
     3781 gtctgttgca gcagccagat caaatccggc gcttccagcg ggtcaatctg catgactttg
     3841 agatcgtaaa tatggcgtga ctctagcaca tattgctgcc cggtgtaggg gttctcgttc
     3901 tgaccgagat agtccgtcag cagttcaaac gggcgcaccg ccgggtttac caccactgcg
     3961 ggcagcataa aacattgtga caaccaggtg gcgtaatatc cccccagtga cgaaccgaca
     4021 atacccagcg aatcgccgcc atgttccagg acaatggatt ccagcagctc tgccgcgtcg
     4081 gaaggatacg gcggcaactg cggaatgatc atctcaacgt cagggtgatg ttccgccagc
     4141 cagtttttta acaagctcgc ttttgcagag cgcggcgagc tgttgaaacc gtgtaaataa
     4201 agaagcgtag acatcagtag ccttctgaag cggtatcagg ttggaaacgt gtgtccgcca
     4261 ggcgatgcac ctcggtggtc agcgtgccat cagcatgtaa ctcgagagta cgccagccgg
     4321 gcgcgatagt atccagcgta aagttggaac agtgcggctt aaactgcaca caggtcgacg
     4381 gcgttgccag caggcggcga ccgttccagt cgagatccag ctcctgatga atatgaccgc
     4441 acagcaagta tttgacgtgc ggaaacttcg ccagcacggt atccagttcg cccgcgttac
     4501 gcagactgtg ttgatcgagc catctacaac ccgcaggtag cggatgatga tgcagcagca
     4561 gcaacgtatg gcgttctggc gcatcggcca gtttacgttc cagccactca agctgaaact
     4621 cgctcagctc accgtgcggc acgccaaaca cctggctatc cagcaacagg atttgccatt
     4681 gctcaccaat aaacacccgc ttcgccgggg agatacccgc atcctgtaac gcgctgtaca
     4741 tcgcgggctg gaaatcgtgg ttgccaggca gccagacgca gggcgcacga aaacttgcga
     4801 tgccttcagc gaaatgctga taggccgcag aggattgatc ctgcgctaaa tcacctgtcg
     4861 cgacaatcag gtcgaattcg tgctggtgtg gacgaatcgc ctccagcacc gcctggtagc
     4921 tttcccaggt atttacccct aacagggctt cgtgcttttg tgcaaacagg tgagtatcgg
     4981 taatttgtaa aatcctgact ctggcctcac cagccagagg aagggttaac aggctttcca
     5041 aatggtgtcc ttaggtttca cgacgctaat aaaccggaat cgccatcgct ccatgtgcta
     5101 aacagtatcg caaccagtcc gctaaaaact gattaatttg atgcttttcg tcgcgttgat
     5161 gcaacttttt attaggataa tcataccgcg ctttgaagcg aaaaatctgc tggcttgaac
     5221 acacttcagc caccatcgcg tcatgataca gacgcaccgt cattgacgga aggctccagt
     5281 aactgatcgc gggcgcagtc tgttctattg tcaccagggt agtgtatcgg gtcgattcca
     5341 caatcgtcag ccgatattgt gcgtttgcca cctgatagct tacagtttcg ccgggtgcgt
     5401 cattgcgcgg taacaaacgg cgcaattgtg aaaagttcat ctcgcacagg cgcatcattt
     5461 caggaaagtc aggtgtgtaa cgcttcattt atgcccactc attttttaac gcttgatgat
     5521 gcagctgcag ccattgcaaa gcgatgaccg acgctgcgtt gtcgattttc ccctcttcta
     5581 cccactggta tgcctgttcc cggcttacca catgaacgcg aatatcttcg ttttcatcag
     5641 ccagaccatg aataccgctt gcggtcgtgg cgtccacttc gcccaccata attgacgaac
     5701 gctcactggt gcctcccggg cttgccagga aacttaacac cggtttggtc cgtttgacta
     5761 tcagtcccgc ctcttcaatc gcttcgcgac gggcaacatc ttccacactt tcaccctctt
     5821 caatcatccc ggcaaccatc tccagtagcc agggggtttc actggtgtcg tatgcggcaa
     5881 tccgaatctg ctcaatcagc acaacttcat cacgcactgg gtcaaagggt agcaagactg
     5941 cggcgtgacc gcgctcaaaa atttcccgcc gtacctcatg actcatttgc ccgttgaata
     6001 aacgatgacg aaatctataa agatctaatg aaaaaaagcc gcgataaagt gtttctcgtg
     6061 caataatttc tacatcgttt ttgccaagtg taacgggcag gttgtctggc ttaagcattg
     6121 ttaatgtcct ggcactaata gtgaattaaa tgtgaatttc agcgacgttt gtctgccgtt
     6181 tgagcagtca tgtgttaaat tgaggcacat taacgcccta tggcacgtaa cgccaacctt
     6241 ttgcggtagc ggcttctgct agaatccgca ataattttac agtttgatcg cgctaaatac
     6301 tgcttcacca caaggaatgc aaatgaagaa attgctcccc attcttatcg gcctgagcct
     6361 ttctgggttc agttcgttga gccaggccga gaacctgatg caagtttatc agcaagcacg
     6421 ccttagtaac ccggaattgc gtaagtctgc cgccgatcgt gatgctgcct ttgaaaaaat
     6481 taatgaagcg cgcagtccat tactgccaca gctaggttta ggtgcagatt acacctatag
     6541 caacggctac cgcgacgcga acggcatcaa ctctaacgcg accagtgcgt ccttgcagtt
     6601 aactcaatcc atttttgata tgtcgaaatg gcgtgcgtta acgctgcagg aaaaagcagc
     6661 agggattcag gacgtcacgt atcagaccga tcagcaaacc ttgatcctca acaccgcgac
     6721 cgcttatttc aacgtgttga atgctattga cgttctttcc tatacacagg cacaaaaaga
     6781 agcgatctac cgtcaattag atcaaaccac ccaacgtttt aacgtgggcc tggtagcgat
     6841 caccgacgtg cagaacgccc gcgcacagta cgataccgtg ctggcgaacg aagtgaccgc
     6901 acgtaataac cttgataacg cggtagagca gctgcgccag atcaccggta actactatcc
     6961 ggaactggct gcgctgaatg tcgaaaactt taaaaccgac aaaccacagc cggttaacgc
     7021 gctgctgaaa gaagccgaaa aacgcaacct gtcgctgtta caggcacgct tgagccagga
     7081 cctggcgcgc gagcaaattc gccaggcgca ggatggtcac ttaccgactc tggatttaac
     7141 ggcttctacc gggatttctg acacctctta tagcggttcg aaaacccgtg gtgccgctgg
     7201 tacccagtat gacgatagca atatgggcca gaacaaagtt ggcctgagct tctcgctgcc
     7261 gatttatcag ggcggaatgg ttaactcgca ggtgaaacag gcacagtaca actttgtcgg
     7321 tgccagcgag caactggaaa gtgcccatcg tagcgtcgtg cagaccgtgc gttcctcctt
     7381 caacaacatt aatgcatcta tcagtagcat taacgcctac aaacaagccg tagtttccgc
     7441 tcaaagctca ttagacgcga tggaagcggg ctactcggtc ggtacgcgta ccattgttga
     7501 tgtgttggat gcgaccacca cgttgtacaa cgccaagcaa gagctggcga atgcgcgtta
     7561 taactacctg attaatcagc tgaatattaa gtcagccctg ggtacgttga acgagcagga
     7621 tctgctggca ctgaacaatg cgctgagcaa accggtttcc actaatccgg aaaacgttgc
     7681 cccgcaaacg ccggaacaga atgctattgc tgatggttat gcgcctgata gcccggcccc
     7741 cgtcgttcag caaacatccg cacgcactac caccagtaac ggtcataacc ctttccgtaa
     7801 ctgatgacga cgacggggct tcggccccgt ctgaacgtaa ggcaacgtaa agatacgggt
     7861 tatctgccgc attcttcccc cttctcgctt caatttcgac cagccatcct ctattctgat
     7921 gggtatttac cactggtccc ggaagacaaa aatgaaacgg acaaaatcca tacgccacgc
     7981 atcgttccgc aaaaactgga gcgcacgcca tctgacacca gtcgctctcg cggttgccac
     8041 tgtttttatg ctggctggct gtgaaaagag tgatgaaaca gtgtctctct atcaaaatgc
     8101 tgacgactgt tcagctgcaa acccaggcaa aagcgccgaa tgtaccaccg cgtacaacaa
     8161 tgcgctgaaa gaagccgaac gtactgcgcc gaaatacgcc acccgtgaag actgtgttgc
     8221 tgaatttggt gaaggtcagt gccagcaggc accagcccag gctggcatgg caccagaaaa
     8281 ccaggcgcag gcccagcaat ccagcgggag tttctggatg ccgctgatgg ccggttacat
     8341 gatggggcgt ctgatgggcg gcggcgcggg atttgcacag cagccgctgt tctcctcgaa
     8401 aaacccagcc agtccggctt acggtaaata taccgacgcg acgggtaaaa actatggcgc
     8461 agcccagcca ggccgcacca tgaccgtacc gaagacggca atggcaccaa aaccggcgac
     8521 caccactacc gttacccgtg gcggttttgg tgaatctgtt gccaaacaaa gcactatgca
     8581 gcgtagtgca accggtacct cttctcgttc aatgggtggc tgataccgat ggaaagagtc
     8641 agtattaccg agcgcccgga ctggcgtgag aaagcccacg aatacggttt caattttcac
     8701 accatgtacg gcgagccgta ctggtgtgaa gatgcttact acaagttgac cctcgcccag
     8761 gttgaaaagc tggaagaagt caccgccgaa ctgcaccaga tgtgcctgaa agtggtggaa
     8821 aaagtgatcg ccagcgatga gctgatgacc aaattccgca ttccaaaaca cacctggagt
     8881 tttgtgcgcc agtcatggct gacgcaccag ccatcgcttt attcgcgtct tgatctggcg
     8941 tgggatggca ctggtgaacc taaacttctg gaaaataacg ccgatacgcc aacgtcacta
     9001 tacgaggcgg cgttctttca gtggatctgg ctggaagatc agcttaacgc cggtaacttg
     9061 ccggagggca gcgaccagtt taacagtctg caagaaaaac tgatcgatcg cttcgttgag
     9121 ctgcgtgaac agtatggctt ccagttgctg catctcacct gctgtcgcga cacggtggaa
     9181 gatcgcggaa ccattcagta tttgcaggac tgcgcaacgg aagctgaaat tgctactgag
     9241 ttcctctaca tcgatgatat cgggttaggt gaaaaaggtc agttcacgga tttacaggat
     9301 caggtaattt ccaacctgtt caagctgtat ccgtgggagt ttatgctgcg tgagatgttc
     9361 tccaccaagc tggaggatgc aggcgtacgc tggctggaac cggcgtggaa gagcattatc
     9421 tccaacaagg cacttctacc gctgttgtgg gagatgttcc cgaatcaccc gaacctgctg
     9481 cccgcttatt ttgcggaaga tgatcatccg caaatggaaa aatatgtggt taagccgatc
     9541 ttctcccgtg aaggcgcaaa cgtgtcgatc attgagaacg gcaaaaccat tgaagcagcg
     9601 gaaggtccgt atggcgaaga aggaatgatt gttcagcaat tccacccgtt accgaaattc
     9661 ggcgacagct atatgctgat tggtagctgg ctggtgaacg atcaacctgc cggaattggc
     9721 attcgtgaag accgtgcatt gatcacccag gatatgtctc ggttttatcc acatattttt
     9781 gttgaataat caatgatacc ggatggcatt cgccatccgg taattgttaa cctacctgca
     9841 ccgacagcat actcaggctg cccatttcta taccctcaac cggaatggta attggctcct
     9901 gcccatccca cgcacctaac acatacaaca acggcagata atgctctggc gttgggttcg
     9961 ataacgtgcc accttcatgg tcgaggtaat tcaccagagg atgttgttcc actggccctt
    10021 gccacgtcag attcgctttc acatactcat taaacgacgt cgcccacgga tacggtgaac
    10081 tatcaccgtg ccacttcact gtgcgcaggt tatgcaccac gttgccactg gcgaccaaca
    10141 ttattccttc atctcgcagc gctgccagtt tgcgccccat ttcgaaatgc caggcggcag
    10201 gtttgctact gtcgatactc aactgcacca tcgggatatc ggcgtcagga tacatcttaa
    10261 tcagcacgcc ccaggaaccg tggtcaaagc cccaggcttc tttatccagc gtcaccggga
    10321 ccggcgctaa cagctcaacc agatgctgtg ccagcacagg cgaacccgga gcaggataat
    10381 gcgtatcgta cagcgcctgc gggaagccac caaagtcatg aatcgtgggc ggcgtctcca
    10441 tcgcggtcac tcctgttcca cgggtaaacc agtgagccga aaccaccaca atcgcttgcg
    10501 ggcgtggcaa tgtcatcccc aacttctgcc agctgcgggt atacaaatta tcttccagca
    10561 cgttcatcgg actaccgtga cctaaaaaca atgctggcat acgtgttgaa gacatgatga
    10621 tatccttaac taaaggtgtc attttgatat cctcacaata cgcttgttcg gcggagtaag
    10681 aacccggata acaatgatga tgatcatcag ttattttgac gatctgcctg aaggtgaaga
    10741 tttataagga gttgtcgatg tcagtacctc tcattctgac catactggcg ggggcagcca
    10801 cgtttattgg cgcgtttctc ggcgttctcg ggcaaaaacc ctcgaaccgc ttactggcgt
    10861 tttcgctagg atttgcggcg gggatcatgt tgctcatctc attaatggaa atgcttcctg
    10921 ccgcactagc agctgaagga atgtcgcctg tgttgggtta tggaatgttt atcttcggtc
    10981 tgcttggcta ttttggcctg gaccgcatgt tgccacatgc gcatccgcag gatttaatgc
    11041 aaaaatcggt gcagccgttg ccaaaatcga tcaagcgcac agccattctg ctcactctcg
    11101 gcatcagtct gcataacttc ccggaaggga ttgccacctt tgtcacggcg agcagcaacc
    11161 tggagctggg atttggcatc gcactggccg tcgcgttgca caatattcct gaaggtctgg
    11221 cggttgcagg cccggtttat gcagcaacgg gttctaaacg cacggcaatt ctgtgggcgg
    11281 ggatttctgg attggcagaa attcttggtg gtgtgctggc gtggttaatc ctcggtagca
    11341 tgatttcccc cgtggtcatg gcggcaatca tggcggcggt tgcaggaatt atggtggcgc
    11401 tctcggttga tgaattaatg ccgctcgcca aagagattga ccctaataat aaccccagct
    11461 atggcgtact atgtggaatg tcagtgatgg gattcagttt agtgctgcta caaacggcgg
    11521 gaattggtta aaagcaacat cagaagttgg ccagcgcatg gacaacttct gctagcttca
    11581 ctaaagcctt attctacctt ttcggctgtc tcttctctcg tactgtttaa gtatttgttc
    11641 cgcgcttcaa gcagtgcgtc tctgtcctgg cgaaacgccc gacaataacg tttcaaatgc
    11701 aggaaataac ccaccacaac aagagcaatc aatacaatcc agtaccaggc gataaacatc
    11761 cttttaccct taatatttaa caatactaaa catttagcgc ataaatttca catatccttt
    11821 ttcggatata ttcatatggt cgcataataa cggaacaatt atatgatttt gatcataatg
    11881 aaaaagaatg cctgatatta atgaaatttt ctcatagaaa gagaagcgga attaacatct
    11941 tgcatagcag ggcaaaataa aaaccgaagc cagctacaac ttcggtttct tgattaaggc
    12001 agtaattaag cagcggtttt cagctggctt tacgctcatg tgcctgacgg tatgccacca
    12061 ggtcttcaat agtcacgagc gccatattgt gtttattggc aaactcaatg cactctggtg
    12121 cacgcgccat cgtgccatcg tcattagtca actcacacag cacaccagcc ggtttaaagc
    12181 ctgccagcgt catcaaatca atagtcgctt cagtatgacc gccacgcgtc agtacaccac
    12241 ctgcctgcgc gcgaagtggg aaaacgtggc ccgggcgatt cagatcagaa ggttttgcgc
    12301 catcggcaat cgctgcgcga acggtcgtaa ttcgatcagc ggcagaaaca ccggtagtca
    12361 caccttcagc cgcttcaatg gtcacggtaa aaccggtgcc ataggcgctg gtgttatttt
    12421 ctaccatcat tggcagatca agttgtttac ggcgatcttc agtgatgcac aggcaaacaa
    12481 taccgctacc gtggcgaatg gtcagcgcca tctgctcaac ggtcatggtt tctgccggga
    12541 agatcatatc gccttcgttt tcacggtctt catcatcaag caccattaca ccgcgtcctt
    12601 cacgcagcgc agccagtgca ttttcaacac gttcgaaagg cgtaccaaaa gaggaaagta
    12661 gcgtctgatt catggtaaaa aaacctcact aaaattatgg ttaccagaat cagggcagtc
    12721 ttaggagtgg cggcatatag ccaaaataac gtgagcgggt ccatgcccga cagaatcgtt
    12781 actctctccc atccggactc taaccgtcgg ccccggaatt acaccggatc tgctgtcctt
    12841 tgagttcgca cccaaagcgc tcgcgggctt tcaactgagt tgatttaccg ccggtgggga
    12901 atttcgcccc gccctgagaa taagcggatt cactataacg ctaatgatta gcggcagcaa
    12961 cgcatagctt cacataattc tggtttatga cttaccctta tcgcactaca atggcactca
    13021 acacctatca gtacagggaa actacaatga ttgacccgaa aaaaattgag caaatcgctc
    13081 gccaggttca cgaatcaatg cctaaaggaa tcagggagtt cggggaagat gtggagaaaa
    13141 aaatccgcca aaccctacaa gctcagctga cgcgcctcga ccttgtaagc cgtgaagagt
    13201 tcgacgtcca aacgcaagtc ctgttacgga ctcgtgagaa actggcgctg cttgaacaaa
    13261 gaatgagcga actggaaaat cgcagtactg agattaaaaa gcagccagat ccagagactc
    13321 tccccccaac gctgtaaaca tttgtcttta atgcactgta aattatgaat taatcttcta
    13381 atttacagtg ctttctatta ccttatcg
//
LOCUS       DAJOWB010000124        13215 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJOWB010000124 DAJOWB010000000
VERSION     DAJOWB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.124"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            9..1943
                     /gene="yegI"
                     /locus_tag="OHO85_003894"
     CDS             9..1943
                     /gene="yegI"
                     /locus_tag="OHO85_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCQ2673206.1"
                     /translation="MKVFTSTGELTTLGRELGKGGEGAVYDIEEFVDSVAKIYHTPPP
                     ALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYSPA
                     HRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDSDS
                     FQINANGTLHLCEVGVSHFTPPELQTLPSFVGFERTENHDNFGLALLIFHVLFGGRHP
                     YSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQAF
                     TESGVATGRPTAKAWVAALDSLRQQLKKCIVSAMHVYPAHLTDCPWCALDNQGVIYFI
                     DLGEEVITTGGNFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYIIL
                     LEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRAKM
                     DYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQKF
                     LEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWKAS
                     CERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMPLM
                     EPLRQAAEKLAQAQADLSRC"
     gene            complement(1955..3307)
                     /gene="yegD"
                     /locus_tag="OHO85_003895"
     CDS             complement(1955..3307)
                     /gene="yegD"
                     /locus_tag="OHO85_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCQ2673207.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSTAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            3441..4289
                     /gene="alkA"
                     /locus_tag="OHO85_003896"
     CDS             3441..4289
                     /gene="alkA"
                     /locus_tag="OHO85_003896"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCQ2673208.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSSVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGRLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            complement(4398..7715)
                     /locus_tag="OHO85_003897"
     CDS             complement(4398..7715)
                     /locus_tag="OHO85_003897"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCQ2673209.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETHFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQVAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMLAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISISLPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGEVIA
                     DAQPLDLLVNSSYFAIN"
     gene            8033..8674
                     /gene="udk"
                     /locus_tag="OHO85_003898"
     CDS             8033..8674
                     /gene="udk"
                     /locus_tag="OHO85_003898"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCQ2673210.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            8766..9347
                     /gene="dcd"
                     /locus_tag="OHO85_003899"
     CDS             8766..9347
                     /gene="dcd"
                     /locus_tag="OHO85_003899"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCQ2673211.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            9369..11222
                     /gene="asmA"
                     /locus_tag="OHO85_003900"
     CDS             9369..11222
                     /gene="asmA"
                     /locus_tag="OHO85_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCQ2673212.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11496..13079)
                     /locus_tag="OHO85_003901"
     CDS             complement(11496..13079)
                     /locus_tag="OHO85_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCQ2673213.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
BASE COUNT         3188 a         3445 c         3511 g         3071 t
ORIGIN      
        1 aaacaaatat aaaagtattt acatcgacag gtgaattgac cactcttggc cgtgaactgg
       61 gcaaaggtgg tgaaggcgcg gtttatgata tcgaggagtt tgtcgatagc gtcgccaaga
      121 tttatcacac gccgccaccc gccttaaaac aggacaaact tgcctttatg gctgcgacag
      181 ctgacgcgca gttgttgaat tatgtcgcct ggccgcaggc aacgcttcac ggtggacgag
      241 gcggaaaagt tatcggtttt atgatgccaa aagtttctgg taaagaaccg attcatatga
      301 tctatagccc ggcacatcgt cgccagagtt accctcattg tgcgtgggat tttctactct
      361 atgttgcgcg caatattgct tcatcttttg ctacggttca cgagcacggg cacgtcgtgg
      421 gtgacgtaaa ccagaacagt tttatggtag gtcgcgacag caaagtggta ctgattgata
      481 gcgactcctt tcaaattaac gccaatggca cactgcattt atgcgaagtc ggcgtgtcgc
      541 attttacgcc gccagagcta caaaccttgc catcatttgt cggttttgaa cgcacagaaa
      601 atcacgataa ttttggcctt gcgttgctaa tttttcacgt cttgtttggt gggcggcatc
      661 cttattctgg tgtgccgctt atctctgatg cgggtaatgc gctggagacg gatattaccc
      721 atttccgtta tgcctacgcg tcagataatc agcgacgtgg tttaaaaccg ccgccacgat
      781 ctattccgct atcgatgtta ccgagtgatg ttgaagccat gtttcagcag gcattcacgg
      841 aaagtggcgt ggcaaccggg cgtccgacgg ctaaagcgtg ggtagcggca ctggactctc
      901 tacgccaaca gttaaagaaa tgtatcgttt cagcaatgca tgtttacccc gctcatttga
      961 ctgactgccc gtggtgtgcg ctggataatc aaggtgttat ctattttatt gatctcggcg
     1021 aagaggtcat taccaccggc gggaattttg tgctggcgaa agtctgggcg atggtgatgg
     1081 cgtcagtagc accgccagca ttgcaactgc cattacccga tcatttccaa ccgactggca
     1141 ggccgcttcc tttaggcctg ttacggcgcg aatacatcat tctgcttgag atcgcactgt
     1201 cagcattatc gctgctgctt tgcggccttc aggcagaacc gcgttatatt attttggttc
     1261 ctgtgctggc ggctatctgg attattggca gtctgacaag caaagcgtac aaagcagaag
     1321 ttcagcaacg ccgtgaggca tttaatcgtg cgaaaatgga ctatgaccat ttagtccgcc
     1381 agatccagca ggtaggcggc ctggaaggtt ttatcgccaa acggacgatg ctcgaaaaaa
     1441 tgaaggacga aattctcggg ttaccggaag aagagaaacg cgctctggca gcacttcatg
     1501 acactgcaag ggaacggcag aagcagaagt ttctggaggg attttttatt gatgttgcct
     1561 ctattcccgg cgttggccct gcgcgtaaag cggcgttacg gtcttttggt attgaaacag
     1621 ccgcagatgt tacccgtcgt ggcgttaagc aagtgaaagg gtttggcgac catctgaccc
     1681 aggcggttat cgactggaaa gcgagctgtg aacgccgttt tgtgttcagg ccgaatgaag
     1741 cgataacgcc agcagacaga caagcggtaa tggcgaaaat gaccgccaaa cgacatcggc
     1801 tggaatcgac gttgactgtc ggcgcgacag agttgcagcg attccgcctt catgctccag
     1861 cacggaccat gccgttgatg gaaccgttac gtcaggcggc agaaaaactg gctcaggcgc
     1921 aggctgattt aagccgctgc tgagccattt tcttttaacg aaataccact tctgcccagc
     1981 gtgccagccc ggcggtgacg gagccaaagt catcgccgcc tgcaatcgga atgcccggca
     2041 actgttctgc cagcgctttt ttaatcagcg gcgaacgggc gctaccgccg gtcaggtaga
     2101 taacgtccgg tttttcctgg gcgttatcca gcgccagttg cacctgttcc agaatccgcg
     2161 ccagtggctg gctgagggcg ctttccagcc cttgctggct aatcagcgta gccagttcat
     2221 cgctgataaa cggcagtgag gcgcgggttt ccgcagtgct tgaaagggcg attttgctct
     2281 cttctgcgct acgcaccagg cgatagctta aacgctgacg ccagactttc tgtaacaggg
     2341 ccactttttc cggttcgcgg gcatcgcgta ccagatcgtt aagcagacga ccgttggcac
     2401 tactgtagaa atcactctgt gcaggaacgt cgttgatggc aaccgcattc caccacggca
     2461 gaatcggcag ggcgatgcct ttttcggttt cgccacccat gcccagtaac ggcatcaggt
     2521 ttttaaacgc cagcgcgata tccagatcgt taccgccaat acggcaacca ctgtgaccca
     2581 gcaggctggc ttcacgatcg agacgcgaac gccactgcgg ccccatcagc agcaatgaac
     2641 agtcagtcgt accaccgccg atatccacca ccagcacccg tttttcttcc tgcaaggtgg
     2701 cttcgtaatc cagcccagcc gcgaccggct cgtactggaa taccacgtcc ctgaatccgg
     2761 cacgcttcgc cgcgcgttcc agaatccctt gcgcctgggt gtttgcttca tcaccgccca
     2821 gcccctggaa gttgatcgga cgaccaatca ccgcctgagt aatcgcttct ggcagctgtg
     2881 cctgcgcctg ctggcgaatg tgcaacatca ttgcgcagac cagatcctca aacagcgcta
     2941 cctgctgcgg ttttaagccg ctggcaccga ggaacgattt tggtgatttc acaaaccaca
     3001 cttcttctgg atcatcaatg tactgtgcca gtgaggaaag accgaactgc acgcttttcg
     3061 ccgtaacatc gatatcttct tcgcggttat aacgaatcgc ccgacgtaac agcgcctgcg
     3121 tttcatcgtc gtctgccgga acatcatgat ggcggtacag ccattcgctt accgcttcac
     3181 gcgttggcgc gcaaagcatt gaaggcagca gcgtgctgtc gttttccatt tttagcaaat
     3241 gcggtttacc gtcacgcatg accgccactg aacagtttgc tgtaccgtaa tcaaaaccaa
     3301 taaacacgaa ataatcccca tgccggtgaa gaaggggcgt gactttagcg aaatgttgcc
     3361 gtcgcgacaa ccggaatatg aaagcaaagc gcagcgtctg aataacgttt atgctgaaag
     3421 cggatgaata aggagatgcg atgtataccc tgaactggca gccgccgtat gactggtcgt
     3481 ggatgttggg atttctcgcc gcccgtgcgg tgagcagtgt ggaaacggtc gcggacagtt
     3541 attatgcccg tagtctggcg gtgggcgaat atcgcggcgt ggtgactgct attccggata
     3601 tagcccgcca tactctgcac ataaatttaa gtgcaggttt agaacctgtt gccgcagagt
     3661 gtctggcgaa aatgagccgc ctgtttgatc tgcaatgtaa cccacaaatt gttaacggtg
     3721 cgttgggcag gttaggcgcg gcgcggcccg gattgcgttt acccggctgt gttgatgctt
     3781 ttgagcaggg cgtgcgggcg attttaggcc aactggtgag cgtggcgatg gcggcaaaat
     3841 tgaccgccag agtggcacag ctttatggcg aacggctgga tgattttccg gagtatatct
     3901 gcttcccgac gcctcagcgg ctggcagcag cagacccgca ggcattaaaa gcgttaggta
     3961 tgccgttgaa acgggcagag gcgctgattc atctggcaaa tgcggcgctg gagggcacct
     4021 taccaatgac aataccgggc gatgtggagc aggcgatgaa aacgctgcaa acttttccgg
     4081 gtatcgggcg ctggacggcg aattattttg ctttgcgtgg ctggcaggcg aaagatgttt
     4141 tcctgccgga tgattatctg attaaacagc gatttccggg aatgacaccg gcgcaaatcc
     4201 gccgttatgc cgagcgctgg aagccctggc gttcttatgc gctgttgcat atctggtata
     4261 cggaaggctg gcaaccagac gaagcatgac gttggtcgcc ggatgcggtg cgagcgcctt
     4321 atccggcctg gggaatgagc gatttgtagg catgataaga cgcgttagcg tcgcatcagg
     4381 cgatggggaa gcacgcctca gttaatcgcg aaataactac tattcaccag caaatccagc
     4441 ggttgggcat cggcaatcac ctcaccataa atcagatcga cgccgatgcc agaaagcgta
     4501 tccatcacta agggtaaaac gactggcccg gcgatggttt tcatcccgag tcgctgagcg
     4561 tgcccctgaa taatcgtaat cagcatctca tccatcaaat taccctgcac gttggcgcat
     4621 aactcaccat caagtagcag gtaatctgcc atattcgctt tcagcgagtt gaagatttgc
     4681 agatcgcggc ccacctggct gagcactatc cgacatcccg ccagccgcag tttttgcacg
     4741 ctttctgcgt gatctaaaat cgcttcagcc ggaataatca gatgtaataa ccgtggtggt
     4801 agagggctat tttccagctg ctcaagcaga tcattcacca gcgtggcgct actcaaaccg
     4861 gcaacggaaa gggggaggga gatgcttatg cctttactgg caaccgcttt tgcggcctgc
     4921 tggaaaaatt cgtggaatac ccggcggtca agagcatggc taagcgccgg atcgctgaag
     4981 ctacgacgaa atgtttgttc atcaataatc tcgccttcgc aactccagag cttaagtgaa
     5041 atcagccaca aattacgcgc ttccgggatc cgtggcgaag cgacaccgtg ggcgagcatc
     5101 atcaactgat tctctttaat catccgccac tgttcatcaa gcgacatcgc cgcccgttcg
     5161 ctatgtgcgg cagcttgctg cggttcgtaa accgtcaccc ggccccggcc accatttttg
     5221 gaggcataac aggcgatatc agcctgcgac atcacttctg ccacctgatg attgttgtca
     5281 tcaatcaagg taatcccggc actggcacct acccgatgca cacggccttc ccatataaag
     5341 tgatagtcat tcacggcact gataatgcgt gtagcgataa aacgcgcgct ttcaacatta
     5401 cagtctggta gcagcagacc aaattcatcc ccaccgagtc gcgccagcac gtcgctggaa
     5461 cgcagcatac tcagcattaa cgacgccagt tcgcgcagca aagcgtcacc cgccgcatgc
     5521 ccggcgctgt cattcaccgc tttaaagcga tcaagatcga taaacaccag ggcatgtcgc
     5581 tgatgtgtac tgtttaccgt ttgcagcagg atacgcagtt gtttctcaaa actggcgcgg
     5641 ttggcgagat gcgtcagtgc atcatgggag gcgctgtagc tcagctggcg cagcattttg
     5701 cgtgattcgg taacgtcctg aatcaccaga accgaaccaa tattgctgcc gtccagagta
     5761 cttaacggcg taatactgta atgcacgtcg taactgccgc cgctccgaca gtgcaacacc
     5821 acatcttgct cgatcgcgga acgtgaggtg tcggcactgt aaatgttctc cattaatggt
     5881 ccgttgtcgc caaaagtaat atgcaacacc gtcaggagcg gaacacctaa cgcttcttcc
     5941 tgcgtccagc cgctcatctt ctccgccact ggattcataa aggtaatttt catcgccata
     6001 tcaatacaga ccacggcttc accgatggaa tcaagcgtaa tgtgcaggcg ctctttttcc
     6061 tgaaacaatg cctcgttaag ctgtttcact tcggtcatat ccatattgat gccgaggaga
     6121 cgttcgactt cgccttcttt attcagtacc cggttggcga gggcgcggat atggcgaata
     6181 ccgtctttta cggtaatgcg aaattccagt ttaaagggcg agcgtgattg caacgaatca
     6241 cgaatcactt tttcggcatg ctggcgatct tccggcagca cgcactcgta ccacacctgc
     6301 cagttcggtt tgatatgtgg aggaatttca tacagctcga acatccgctt atcccagcta
     6361 aaaatattcg gcttcaactc ccactcccag atgccaatcc cgcccgcttc gttagccaga
     6421 gtgatgcgct ccatcagttg ctgattgacc tgttcggtgc gttttagctc gttaatgtct
     6481 tcaatctgag cgataaaata gagcggcgtg ccatccgtgt ggcgcaccag tgagacggca
     6541 agcaacgccc agacaacatc gccattgcgg ttgtagtagc gtttttccat tgaataggtg
     6601 tttatttcac cgcttatcag cttttcaacc tgttggagat ctttattgag atcctccggc
     6661 caggtcagtt gctgaaaggt gagtccgcgc agctcttcct gactgtaccc gagaaactgg
     6721 cagagcgctt tgttggtttg cagccattgt ccctcggtgc ccactaacgc cataccgata
     6781 gcggaatatt ccatcgcgtt ccgaaagtgg gtttcgcttt cggaaatgtg tttgcgttcc
     6841 gcacgaaagg catacatcac catggtcatg atgttggcgg gcagcaggat cagcaaaaac
     6901 ggtagccacg gcatatggct catcaggtac gtacgcggcg tagcaagcag ggagggatcc
     6961 gcggccatca tcagcgacac catcatcacc gtggtaagga agatcaaaaa ggcttccatt
     7021 cgtggcagac gcacggcgct ccacatcaac agcacaataa tgaaagtaaa aggccacggc
     7081 agatacagca tcgaaagcca gcttaacgtc agtgtgatgg ctaacgtgag cagcgactca
     7141 aaaagcaacc gtgggttgcg atggcgcagc agatagtgtg gtttaaataa caatcccagc
     7201 ggcaccagtg ccagagcgcc gatggattct gacagtaccc atatcaaaaa tgccctgaga
     7261 ggatcgtctc cgggcgtcag caggacaacc agaacacccc ccaacagagg cggaacaatg
     7321 gcgctgccga gtgccagacg cagccagtca gccagatttt gcaaggggtt ataccacggc
     7381 agcaatttac gtagcagcac tgccccgacc acggcttcaa caatattgat ggtcgtccag
     7441 gtcatgttca gcgagctggt ggaaaaaagc aggatggatg cggcgatatt tcccagcgaa
     7501 cagctcagcg caattcccgg ccacatgcgc ccggcatggc gataaaacgc caccatcatg
     7561 atggacgtcg ggaaccacag tggggcgagt tgggtgccaa actgcgaaag ctcaagcgag
     7621 aaaagggtaa agataaacga gactaaacct aaactgacca ggtgaagcag cgggtggggc
     7681 agggcaatta atacatgctg tgattgtttg ctcattaccg ctttatccgc aagacctggt
     7741 cgccagtatt cgtttctgtg tttatgctag tacaaaagtt ttacatttta tatgcgaatt
     7801 gctcataaag tgacgtaaag gcggataatt tgcgcaactg cgtataacat tttttacctt
     7861 acataaaact gatcaacgta atttgccccc gggaattctt tgtcaacaat taactctgag
     7921 cgcgcggcaa cgctattcga ctggtatcag acggatgaaa tccctataat tgccgcgttt
     7981 ggcgcttcgt cgcccccttc ctaacatcca ggttaatcag gtcgctaaat atatgactga
     8041 tcagtctcat cagtgcgtca ttatcggtat cgctggcgca tcggcttccg gcaagagtct
     8101 tattgccagt accctttatc gtgaattgcg tgagcaagtc ggtgatgaac acatcggcgt
     8161 aattcccgaa gactgctatt acaaagatca aagccatctg tcgatggaag aacgcgttaa
     8221 gaccaactac gaccatccca gcgcgatgga tcacagtctg ctgcttgagc atttacaagc
     8281 gttgaaacgc ggctcggcaa ttgacctgcc ggtttacagc tatgttgaac atacgcgtat
     8341 gaaagaaacg gtgacggttg agccgaagaa ggtcatcatt ctcgaaggca ttttgttgct
     8401 gacggatgcg cgtttgcgtg acgaacttaa cttctccatt ttcgttgata ccccgctgga
     8461 tatctgcctg atgcgccgca tcaagcgtga cgttaacgag cgtgggcgtt caatggattc
     8521 agtgatggcg caatatcaaa aaaccgtgcg cccgatgttc ctgcaattca ttgagccttc
     8581 taaacaatat gcggacatta tcgtgccgcg cggcgggaaa aaccgcatcg cgatcgatat
     8641 attgaaagcg aaaataagtc agttctttga ataagcttga taaattgtgt accgttcagt
     8701 gataacctgg tatgcccttg acgtaattgg cgttaaggga gtgatgcgcg aaaggagaaa
     8761 atgccatgcg tctgtgtgac cgagatattg aagcctggct tgatgaaggc cgtttgtcga
     8821 tcaacccacg tccgccagtg gagcgtatta acggcgcgac ggtggatgta cgcctgggca
     8881 ataaatttcg taccttccgt ggtcacacgg cagcgtttat cgatctgagc ggtcccaaag
     8941 atgaagtgag cgccgcgctt gaccgcgtga tgagcgatga gatcgttctc gacgagggcg
     9001 aggcgttcta tcttcaccca ggagagctgg cgctggcggt gacgctggag tcggtgacgc
     9061 tgccagccga tctggtgggc tggctggacg ggcgttcctc actggcgcgt ctggggctga
     9121 tggtgcacgt caccgcgcac cgcatcgatc cgggctggtc tggttgcatt gtgctggagt
     9181 tctacaactc cggtaagctg ccgctggcgc tgcgtccggg catgttaatt ggtgcgctga
     9241 gctttgagcc gctttccggc ccggcggcgc gaccttacaa ccgccgtgaa gatgcgaaat
     9301 atcgcaacca gcagggcgcg gtagccagcc gaatcgataa agactaattc aggcccattg
     9361 aggataccat gagacgattt ctgacgacgc tgatgatact cctggtcgtg ctggtggccg
     9421 ggttatctgc gttagtgttg ctggtgaatc cgaatgattt ccgcgactat atggtcaagc
     9481 aagttgctgc acgtagcggt tatcaattgc agctcgacgg gccactgcgt tggcacgtct
     9541 ggccgcagct tagtatcctc tccgggcgaa tgtctctcac cgcccagggc gcaagccagc
     9601 cactggttcg cgccgacaac atgcgtctgg acgtggcgct tttaccacta ctgagtcatc
     9661 aactgagcgt taagcaggtg atgctaaaag gggcagtgat ccaactgacg ccgcagacgg
     9721 aagcggtgcg cagtgaagac gctccggttg caccgcgcga caataccttg ccggatctgt
     9781 cagacgatcg cggatggtcg tttgatatat ccagtcttaa ggtggcggac agcgtgctgg
     9841 tgttccagca tgaagatgac gagcaggtga caatccgcaa tattcgcctg caaatggaac
     9901 aagatcccca acatcgtggt tcatttgagt tctccgggcg ggttaatcgc gatcagcgcg
     9961 atctcacgat atcccttaac ggtacggtag atgcttctga ttatccgcat gatttaacgg
    10021 cggctattga acaaattaac tggcagttgc agggtgccga tttaccaaaa caaggtattc
    10081 aggggcaggg gagtttccag gcccagtggc aggagtcaca taaacgcctt tcatttaacc
    10141 aaattagttt gaccgccaat gatagtacgc taagcgggca agcacaggtt acgctgacag
    10201 agaaaccgga atggcagctg aggctgcaat tcccgcaact gaatcttgac aacctcatcc
    10261 cgcttaatga aaccgcgaat ggtgaaaacg gtgccgcgca gcaggggcag agccaatcaa
    10321 cgttgccgcg cccggtcatt tcttcgcgta ttgatgaacc ggcctatcag ggactgcaag
    10381 gctttacggc tgatattttg ttgcaggcca gtaacgtgcg ctggcgcgga atgaatttta
    10441 cagatgttgc cacgcaaatg accaacaagt cgggtttgct ggaaattact caactgcagg
    10501 gcaaacttaa cggtggacag gtttcactgc cgggcacgct ggacgcgaca tcaataaatc
    10561 cgcggataaa cttccagcca cggctggaaa acgttgagat tggtaccatt ctgaaggcgt
    10621 ttaactatcc gatttcgttg accggaaaaa tgtcactggc tggtgatttc tccggtgctg
    10681 acatagatgc cgacgcattc cgccacaact ggcaaggaca ggcacatgtc gaaatgaccg
    10741 acacgcgcat ggaagggatg aacttccagc agatgattca gcaagcggta gagcgtaatg
    10801 gcggtgatgt gaaggccgct gaaaactttg ataacgtgac gcggcttgac cgctttacca
    10861 ccgatttgac gttgaaggat ggcgtcgtga cgttaaacga catgcaaggt caatcgcctg
    10921 tgctggcgct gacaggggaa ggcatgttga atctggcaga tcaaacctgc gacacccagt
    10981 ttgatattcg ggtcgtgggt ggctggaacg gggaaagcaa actgattgat ttcctgaaag
    11041 aaacgccagt accgctgcgg gtttatggca actggcagca actcaattac agcctgcaag
    11101 tggatcagtt actgcgcaaa catctacagg acgaagcgaa acgtcgcctg aatgactggg
    11161 ccgagcggaa taaagattcc cgtaatggca aagatgtgaa gaagttgctg gagaagatgt
    11221 aggttaccag gtgaccagtc ctgtaggcct gataagacgc gtaaagcgtc gcatcaggcg
    11281 ttcgtggcgg catcattata ttctgaatat gtaagatcga aggctgttcc gttcacttga
    11341 tgttcctcgc tactatggaa cgctcaagcc ggtgaacgtg ccagggtggc tcaatctcca
    11401 ccaccctggc aacccgggct cccggcggta aatcgccgat ggcgcggttc cctcatctta
    11461 ttcctttcgg ctattgggta aaatgaccga taccatcaca cctcgtactc catctcgcca
    11521 tccttacgca gcggtataat ctgcaccttc tgcacgcgat ggctttctac ctgcaacgtt
    11581 ttaagcaaat aatcccccac ctgaacttct tcgccaggtt ttggaatacg ctgcaaatac
    11641 tccatcaaca gcccggcaat ggtgtgatat tcacgttttt catccagcgg cagcggcaca
    11701 tattgcacca gatcctccag cggcatatga ccattcgccg tccaggaacc gtccgcattc
    11761 ttctgaatat catggcgggc gtcgatctct tccacttcgt tcggtaagtt accggcaatg
    11821 gtttcagtga cgtcacttaa tgtcacaatc ccttccaccg agccaaactc atccaccaca
    11881 aaagcaaagt gcgtgcgggc attacggaac tgctccaggg caggtaacaa cggcaaggtt
    11941 tccgggaaca ccagcggctg gcgaatcaac acccgcaggt tgagcggttc gccgcgcaga
    12001 gactgttgta gcaggtcgat aacgtgaaca acaccgagca aatcttctgc gtcatcaccg
    12061 tcggtaacca ccagccgcgt atgctgattt cgctccagca actggcggat ttcttcttcc
    12121 ggcgcgttga gatcgatatg ctcaatatcg tggcgcgacg tcataatgct gctgacggta
    12181 cgctggttaa gattaagtac ccgctcaatc atccgccgtt cctgcggatt aaagatctgc
    12241 tggttaccat gatccaccag catggacgcg gtttcggcgt ccagttccgc atcttctttt
    12301 tgcccgctaa gcaggcgcat taccgcttcg gtagtccgct ggcgcagcgt ctggttagcg
    12361 gaaagaaaac gtcgccggtt aaagatagcc aactgattga gcgcctcgat catcaccgag
    12421 aaaccaatgg cagcgtacag gtagcctttc ggaatgacga agccgaaacc ttctgccacc
    12481 aggctaaagc caatcatcaa caggaagctt aagcagagaa taacgatcgt cgggtgactg
    12541 ttaacaaatt gcgttaacgg cttgctggcc atcaacatca ggctgatagc gataaccacg
    12601 gcggccatca tgaccagtaa atggtcgacc atcccgacgg cggtaatcac tgagtcgagt
    12661 gagaaaatgg cgtccagtac cacaatttgt gtcaccaccc cccagaactt cgcacctttg
    12721 cgttgtgtgg gattattgct gtctttccct tccagccgtt cgttcagctc catcgtggct
    12781 ttgaacaaca ggaagaaacc accaaacagc atgattaagt cgcgggcgct aaaggtaaac
    12841 gagcggaagc tgaacagcgg ttgagtcagg gtgaccagcc aggagattga cgccagcagt
    12901 aacaggcgca ttaacatcgc cagcagcagc ccggtaaccc gtgcgcggtc gcgctgcttc
    12961 ggcggtagtt tttcggcgag gatggcaata aagaccaggt tatcaatgcc gaggaccagt
    13021 tcgatcacaa tcagcgtgat taacccggcc cagatagacg gatcggcaat ccattccata
    13081 gtaagtacaa aacctattcg ttgtatgaca aatgtcacaa ttcgatcatg gataaatcgg
    13141 ggcaaaaatg caatgttgca gcactattta tctcatatta tttgagggta ataacctcac
    13201 attatccctg aatta
//
LOCUS       DAJOWB010000125        12962 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJOWB010000125 DAJOWB010000000
VERSION     DAJOWB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.125"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..152
                     /locus_tag="OHO85_003902"
     CDS             <1..152
                     /locus_tag="OHO85_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HCQ2673214.1"
                     /translation="EKTVDAAKICGGAENVVKTETQQTFVNGLLGFITFGIYTPLEAR
                     VYCSQ"
     gene            complement(210..351)
                     /locus_tag="OHO85_003903"
                     /pseudo
     CDS             complement(210..351)
                     /locus_tag="OHO85_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            962..2061
                     /locus_tag="OHO85_003904"
                     /pseudo
     CDS             962..2061
                     /locus_tag="OHO85_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356822.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3297..4412
                     /gene="iroB"
                     /locus_tag="OHO85_003905"
     CDS             3297..4412
                     /gene="iroB"
                     /locus_tag="OHO85_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin biosynthesis C-glycosyltransferase
                     IroB"
                     /protein_id="HCQ2673215.1"
                     /translation="MRILFVGPPLYGLLYPVLSLAQAFRVNGHEVLIASGGQFAQKAA
                     EAGLVVFDAAPGLDSEAGYRHHEAQRKKSNIGTQMGNFSFFSEEMADHLVEFAGHWRP
                     DLIIYPPLGVIGPLIAAKYDIPVVMQTVGFGHTPWHIKGVTRSLTDAYRRHNVGATPR
                     DMAWIDVTPPSMSILENDGEPIIPMQYVPYNGGAVWEPWWERRPERKRLLVSLGTVKP
                     MVDGLDLIAWVMDSASEVDAEIILHISANARSDLRSLPSNVRLVDWIPMGVFLNGADG
                     FIHHGGAGNTLTALHAGIPQIVFGQGADRPVNARVVAERGCGIIPGDVGLSSNMINAF
                     LNNRSLRKASEEVAAEMAAQPCPGEVAKSLITMVQKG"
     gene            4552..8211
                     /gene="iroC"
                     /locus_tag="OHO85_003906"
     CDS             4552..8211
                     /gene="iroC"
                     /locus_tag="OHO85_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin/enterobactin export ABC transporter
                     IroC"
                     /protein_id="HCQ2673216.1"
                     /translation="MPANHTPTPAQSWIVRLARVCWERKKLSVIVVVASVSTILLAAL
                     TPLLTRQAVNDALAGNPARLPWLACGLLLIAFFDFIGNYVRRGYAGMLSLWVQHTLRG
                     RVFDSIQKLDGAGQDALRTGQVISRTNSDLQQVHTLLQMCPVPLAVFTYYIAGIAVML
                     WMSPAMTLIVVCVLVCLAITALRARRRVFAQTGLASDQLANLTEHIREVLAQISVVKS
                     CVAEMRETHWLDRQSRQIVRVRIGAVISQAMPGATMLALPVLGQIVLLCYGGWSVMHG
                     RIDLGTFVAFASFLAMLTGPTRVLASFLVIAQRTQASVERVFALIDTRSQMEDGTESI
                     NSQVVGLELENMSFDYHHGDRHILSNISFSLRAGETVAVVGASGSGKSTLLMLLARFY
                     DPCSGKIWLNTSEGRQNLRDIRLEALRRRVGIVFEDAFLFAGTVAENIAYGHPQATAD
                     DIRRAAAAAGASDFINALPKGFDSLLTERGTNLSGGQRQRIALARALITAPDVLILDD
                     TTSAVDAVTEAEINTALGRYADEGHMLLVIARRRSTLQLASRVVVLDKGRMVDTGTPA
                     ELEARCPAFRALMTGDSDFLATSHNSHNELWPAEPATQDDVTDTGDKGFVARMTRVPE
                     NAVQQALAGKGRKVTSLLKPVAWMFVIAALLIALDSAAGVGVLILLQHGIDSGVAAGD
                     MSIIGLCALLALCLVIVGWCSYSLQTVFAARAAESVQHSVRLRSFGHMLRLGLPWHEK
                     HADSRLTRMTVDVDSLARFLQNGLAGAATSLVTMFAIAATMFWLDPFLALTALSAVPV
                     AALATMIYRRLSTPAYAQARLEIGKVNSTLQEKVSGLRVVQSHGQQELEGARLRALSE
                     RFRATRVRAQKYLAVYFPFLTFCTEASYAAVLLVGASQVAAGEMTAGVLAAFFLLLGQ
                     FYGPVQQLSGIVDAWQQATASGKHIDELLATEGTENLGSSSVLPVTGALHLDEVTFSY
                     PDSHEPALNKLTLTIPEGMVVAVVGRSGAGKSTLIKLIAGLYFPTHGNIRIGVQMLDD
                     ASLTEYRRQIGLVDQDVALFSSDIAENIRYSRPSATNEDVEIASQRAGLYEMVCNLPQ
                     GFRTPVNNGGADLPAGQRQLIALARAQLANAHILLLDEATSCLDRTSEERLMSSLTDV
                     VHAGKHSALIVAHRLTTAQRCDLIAVIDKGLLAEYGTHEQLLSAGGLYTRLWHDSVSS
                     TALHRQHNMKEETPG"
     gene            8315..9544
                     /gene="iroD"
                     /locus_tag="OHO85_003907"
     CDS             8315..9544
                     /gene="iroD"
                     /locus_tag="OHO85_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroD"
                     /protein_id="HCQ2673217.1"
                     /translation="MLNMQQHPSAIASLRNQLAAGHIANLTDFWREAESLNVPLVTPV
                     EGAEDEREVTFLWRARHPLQGVYLRLNRVTDKEHVEKGMMSALPETDIWTLTLRLPAS
                     YCGSYSLLEIPPGTTAETIALSGGRFATLAGKADPLNKMPEINVRGNAKESVLTLDKA
                     PALSEWNGGFHTGQLLTSMRIIAGKSRQVRLYIPDVDISQPLGLVVLPDGETWFDHLG
                     VCAAIDAAINNGRIVPVAVLGIDNINEHERTEILGGRSKLIKDIAGHLLPMIRAEQPQ
                     RQWADRSRTVLAGQSLGGISALMGARYAPETFGLVLSHSPSMWWTPERTSRPGLFSET
                     DTSWVSEHLLSAPPQGVRISLCVGSLEGSTVPHVQQLHQRLITAGVESHCAIYTGGHD
                     YAWWRGALIDGIGLLQG"
     gene            9629..10585
                     /gene="iroE"
                     /locus_tag="OHO85_003908"
     CDS             9629..10585
                     /gene="iroE"
                     /locus_tag="OHO85_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroE"
                     /protein_id="HCQ2673218.1"
                     /translation="MYAREYRSTRPHKAIFFHLSCLTLICSAQVYAKPDMRPLGPNIA
                     DKGSVFYHFSVTSFDSVDGTRHYRVWTAVPNTTAPASGYPILYMLDGNAVMDRLDDEL
                     LKQLSEKTPPVIVAVGYQTNLPFDLNSRAYDYTPAAESRKTDLHSGRFSRKSGGSNNF
                     RQLLETRIAPKVEQGLNIDRQRRGLWGHSYGGLFVLDSWLSSSYFRSYYSASPSLGRG
                     YDALLSRVTAVEPLQFCAKHLAIMEGSATQGDNRETHAVGVLSKIHTTLTILKDKGVN
                     AVFWDFPNLGHGPMFNASFRQALLDISGENANYTAGCHELSH"
     gene            complement(10630..12807)
                     /gene="iroN"
                     /locus_tag="OHO85_003909"
     CDS             complement(10630..12807)
                     /gene="iroN"
                     /locus_tag="OHO85_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore salmochelin receptor IroN"
                     /protein_id="HCQ2673219.1"
                     /translation="MRINKILWSLTVLLVGLNSQVSVAKSSDDDNDETLVVEATAEQV
                     LKQQPGVSVITSEDIKKTPPVNDLSDIIRKMPGVNLTGNSASGTRGNNRQIDIRGMGP
                     ENTLILIDGVPVTSRNSVRYSWRGERDTRGDTNWVPPEQVERIEVIRGPAAARYGSGA
                     AGGVVNIITKRPTNDWHGSLSLYTNQPESSDEGATRRANFSLSGPLAGNALTTRLYGN
                     LNKTDADSWDINSPVGTKNAAGHEGVRNKDINGVVSWKLNPQQILDFEAGYSRQGNIY
                     AGDTQNSSSSAVTESLAKSGKETNRLYRQNYGITHNGIWDWGQSRFGVYYEKTNNTRM
                     NEGLSGGGEGRILAGEKFTTNRLSSWRTSGELNIPLNVMVDQTLTVGAEWNRDKLDDP
                     SSTSLTVNDSDISGISGSAADRSSKNHSQISALYIEDNIEPVPGTNIIPGLRFDYLSD
                     SGGNFSPSLNLSQELGDYFKVKAGVARTFKAPNLYQSSEGYLLYSKGNGCPKDITSGG
                     CYLIGNKDLDPEISVNKEIGLEFTWEDYHASVTYFRNDYQNKIVAGDNVIGQTASGAY
                     ILKWQNGGKALVDGIEASMSFPLVKDRLNWNTNATWMITSEQKDTGNPLSVIPKYTIN
                     NSLNWTITQAFSASVNWTLYGRQKPRTHAETRSEDTGGLSGKELGAYSLVGTNFNYDI
                     NKNLRLNVGVSNILNKQIFRSSEGANTYNEPGRAYYAGVTASF"
BASE COUNT         3062 a         3382 c         3387 g         3131 t
ORIGIN      
        1 aagagaaaac tgttgatgca gccaaaattt gtggcggtgc agaaaatgtt gttaaaacag
       61 aaactcagca aacattcgta aatggattgc tcggttttat cacttttggc atctatactc
      121 cgctggaagc ccgggtatat tgctcacaat agttgcccat cgatatgggg agctcatctg
      181 cactgttcat taatatactt ctgggctccc tacagttgtt tttgcatagt gataagcctc
      241 tctctgaggg aggaaataat cctgttcagc gatgtctgcc agtcgggggg ctgcattatc
      301 cacgcccgag gcggtggtgg cttcacgcgg ggatgggcag attgatctga tatgcaaccg
      361 acgacgacca gcggcaacat catcacgcag agcttcattt tcagatttgg gccacctttt
      421 gatttctcgt ttaacttttg cagtcaccag actgtgattt attttaacaa atcaatagct
      481 ttcataaaat tctcgaagct gaaagcttaa ctgaaccaac cagccaggct ggtgttttct
      541 aaagacaaaa aggccatcag caaaacactg atagtctgaa tcacctttat caatcatgta
      601 tgaagaaaat atgcgcaatt agttgacagt gattatcatt ttcattaaaa agagcgcgta
      661 gcattcttat ttcatgagga aatttacccg ccaacaacct gagtagcgaa agctgttcat
      721 ccccaatatt tttgctggcg ggttcttttt tctactgtcc tcctccccag aggaagcatt
      781 cccattaatc attactggcg aggaggcaag agaaaaagtc ggttctttgt ccctaaataa
      841 cagcatcatt tcagtccatg caaaatctga tttaccagct cacataataa cctgatgata
      901 cacagtgtaa gttcacagag atattgcaat tgcctccgga taagtaaggg gagattgcac
      961 tatgcaaatg cagcacctga ggttggctat cctaagcagc cgattatgcg ttgaggcttt
     1021 cagtgatggc tgatatagca acaatgagaa tgaaggctct gcacttctgg gataaacacg
     1081 gtatttctgc agcttctgaa gcctttggcg tatcctgccg cacgctttac tggtggcgtc
     1141 agttactgaa caagggagga cctgaggggc taatcccgca cagtaaggca cctctggtgc
     1201 gacgaaaaaa gcactggcat cccgatgtgc tgaaagagat tcgacgcctc aggacagagc
     1261 tgccgaacct cggtaaagag cagatttttg ttcgcctgaa gccttggtgc gcataacgcc
     1321 atcagacctg cccgagtgtt tccaccatcg gcagaatgat cgccaccgca cacgataaaa
     1381 tgcggatgat accagtacgt ctgagctcgc ggggtaaggc tctgcttgtc aaaaagcgaa
     1441 ccaccaaccc cccgcaggcc aaagcactac cgtccggtaa agacaggaga gctcattggg
     1501 atggacgcca ttgagctcag aatgggtgaa atgcgtcgct atatcattac catgatcgac
     1561 gaacacagcg attatgtact ggcgctggct gtgccgtcgc tcaacagtga tatcgtcaat
     1621 cactttttca gccgtgcagc ccggctgttc cctgtcggta tcagccagat catcacagat
     1681 aacgggaaag agttcctggg aagctttgac aaaacgctgc aggaagccgc catcaaacac
     1741 ctctggacct atccctacac gccaaaaatg aacgctatct gtgaacgttt taaccgggca
     1801 ttaagagagc aattcattga atttaatgaa attttactct ttgaggatct ggcgttgttt
     1861 aacctgaaac tggcggaata tctggcgctg tataacagca aaagactcca caaggcgctc
     1921 gcactaacga cgcccgtgga atatatttta aaagagaaca aaaattgcaa tatgtggtgg
     1981 acccatacac agcaaacgca caggccgccc cggttgggta gatatgtggg atggctggtt
     2041 cagacttcag gatatggttg aggctggtga tgaagtctct ttatcaggag atctgatcaa
     2101 gagacagccg cgcaggggga ttcactatgc tggattcttt taacaaattc tccgctaacc
     2161 aaaaaataga ctttttgcat tcgtaaatgc gttaagagat aatttttaaa gttccatcaa
     2221 tctcccggcc agttcgtctc gcggcattac tcactatccg cacaacttca gtaagatcat
     2281 gactttttca atcgttatct gttgaccaag ctttgaaacc actagcgtcc cgtcaacctg
     2341 acctggaatc gtcgagaacc gcccatagtt gtgaattggc tagagtgtga tggaaagcaa
     2401 cgctaaacga cctagtcaca cattctcttt tccctaacaa tgaccattta gtgaaataac
     2461 gtatccaaaa tgatgacgtg aaagcctgac actggcagcg aaaagccgaa aaaactcacc
     2521 tggtcaggag caaaaatcgg attattcgcc aacagcgtaa tatcagcagt agttctcgac
     2581 acgcttgcgc tatagacaga ggatgaaacg ctgctggtcg gagttctcta tacatttttg
     2641 gcgacggcag aaccgctcac caatgcctta acgtctatca gccttccggt gagttggaac
     2701 agcatgctca gcgtttgcaa gatattctcc acggtctgta gcagcgtagg tttcaattta
     2761 aatatcccca gatagccctc tgggagcttc cctttttatt aagttttaat taccgttgca
     2821 atagataaat gatattggta attattatca ttagcatttg tgtctgtgtt gtttctggta
     2881 aggctgaaag ggaacaaaca tcgcctgtac aacggcgttt ttacttacag accagcgaag
     2941 gcgagactat caggatgatg catcgtgtgt ctaagaaatc acggttcttt ccagattcgt
     3001 tgttcggtct aacactgcac aaatgcgtca gaacatcccg gcgtagctgt ccgacgccat
     3061 ttgggctgtt caacaggaca atggcgcgta agcgttaatg gaagtgggga tacggagttc
     3121 cctgttcacc tgtgtatgtc acggaaaagt ctctgactga gctgtccata acgctggtga
     3181 agcacaaatg gcgcagcatc tggtaaagca ggtttaacga aaagtcgtgt cctgctgctg
     3241 gcatatgagt gcgtcgactg cctgatttag atcgtcaagc ggagagggat tttctcatgc
     3301 gtattttgtt tgtaggcccg cctctctatg ggctgttgta tcctgtgctg tctctggcgc
     3361 aggcatttcg cgttaacggt catgaagtgc tgatcgccag tggaggacaa ttcgcacaaa
     3421 aagcggcaga ggccgggttg gtggtgtttg acgccgcacc cggtctggat tccgaagctg
     3481 gttaccgcca ccatgaggca cagcggaaaa aaagtaatat cggaacccaa atgggcaact
     3541 tctcattctt cagcgaagaa atggccgacc atctggtgga gtttgccgga cactggcggc
     3601 ctgacctcat catctaccct ccgctcggag tcatcgggcc gctgattgcc gccaaatatg
     3661 atataccggt cgtgatgcaa accgtaggct tcggccatac cccctggcat atcaagggtg
     3721 taacgcgttc gctcacggac gcctatcgtc gccacaatgt gggggccaca ccacgtgata
     3781 tggcctggat cgacgtaaca ccgccgagta tgagcattct ggaaaacgac ggtgaaccca
     3841 ttattccgat gcagtacgtc ccgtataacg gtggcgcggt gtgggaacca tggtgggaga
     3901 gaaggcccga gcgtaaacgt ctgctggtga gtctcggcac tgtcaaaccg atggttgacg
     3961 gccttgatct aatcgcctgg gttatggatt ctgccagtga ggtcgatgcc gagatcatcc
     4021 tgcatatttc agccaatgcg cggtccgatt tgcgctcgct gccgtcaaat gttcgcctgg
     4081 ttgactggat acccatgggt gtcttcctca acggcgcaga tggtttcatt catcatggcg
     4141 gagcaggcaa taccctgaca gcgctacacg ctggtattcc acaaatcgtc ttcggccagg
     4201 gagccgatcg cccggtaaat gcccgcgttg ttgccgaacg cggctgcggg attatccctg
     4261 gcgacgtcgg cctgtcgagt aacatgatca atgctttcct caacaatcgc tcacttcgca
     4321 aagcctctga agaggtagca gcggaaatgg cggcgcaacc ctgcccaggt gaagtggcga
     4381 aaagcctgat cacaatggta cagaaagggt aaccactaca ccttcatgat aatcatatgc
     4441 cagcacccat ccaggagtca tgcatccata acaatgcggg accggagcgt gctggctttt
     4501 ttgcggaacg atccggccag tggatcgtat tttacagacg gaggaggctt aatgcctgcg
     4561 aatcacactc ccacaccggc tcagtcatgg atagttcgcc tagcgcgcgt gtgctgggaa
     4621 cgtaagaaac ttagtgtcat cgtggtggta gcgtcagtat cgactatttt gctggctgcg
     4681 ctgacgccac tgctgacaag acaggctgtg aatgacgcac tggcgggcaa tccggcccgc
     4741 ctgccgtggc tggcctgcgg gttactgttg atcgcttttt ttgatttcat cggtaactat
     4801 gtgcgccgtg gttatgccgg gatgctctca ctctgggtgc agcataccct cagaggacgg
     4861 gtattcgaca gtattcagaa acttgacggc gcaggccagg atgcgctgcg caccgggcag
     4921 gtgatttcac ggaccaacag cgatctgcag caggtgcata ccctgctgca gatgtgcccg
     4981 gtgccgctgg cagtgttcac ttattacatt gccggcattg ccgtgatgct gtggatgtcc
     5041 cctgccatga cgcttatcgt cgtgtgcgta ctggtatgcc tggcgatcac cgcgcttcgt
     5101 gcgcgtcgta gggtcttcgc gcaaaccggg ctggcctcgg accaactggc gaatctcacc
     5161 gaacatatac gcgaggtgct ggcacagatc tcagtggtaa aatcctgtgt ggcagagatg
     5221 cgtgaaacgc actggctcga taggcagtcg cggcagattg tgcgtgtacg catcggtgcg
     5281 gttatctcgc aggcgatgcc tggggccacc atgctggcgc taccggtgct cgggcaaatc
     5341 gtcctgctgt gctacggcgg gtggtcggtc atgcacgggc ggatcgatct cggtaccttc
     5401 gttgcattcg ccagcttcct cgcgatgctg accgggccaa cccgcgtact ggcatcgttt
     5461 ctggttatcg cacagcgcac tcaggcgtcc gtggagcggg tgtttgcact gatcgacacc
     5521 cgttcacaga tggaggacgg gacggagtcg attaacagtc aggttgtcgg actggaactg
     5581 gagaatatga gctttgacta ccaccatggc gacagacata tcctcagcaa tatctccttt
     5641 tccctgcgcg ccggtgaaac cgtggcggtg gtgggcgcat cgggttcagg aaaatcgacc
     5701 ctgttgatgc tgctggcgcg tttttatgat ccctgctccg gaaagatatg gctcaacacc
     5761 agcgaaggcc gacaaaatct tcgcgatatc agactggagg cgcttcgtcg ccgggtaggc
     5821 atcgtatttg aagatgcttt tctgtttgcc ggtacggtgg cggaaaatat cgcctatggc
     5881 caccctcagg caacggcgga cgacattcgc cgtgcggcag ctgctgcagg agccagcgat
     5941 tttatcaacg ccctgccgaa aggcttcgat agcctgttaa ccgaacgggg tacgaatctt
     6001 tccggcgggc agaggcagcg aatagcgctg gcgcgggcgc tcattactgc accggacgtg
     6061 ttaatcctgg atgataccac ctcagcggtt gatgctgtta cggaagcgga gattaatacc
     6121 gcgctgggtc gctatgctga cgaagggcat atgctgctgg tgattgcccg acggcgttca
     6181 acacttcagc tagccagccg ggttgtggtg ctggataagg gccgtatggt ggataccgga
     6241 accccggcag aacttgaagc gcgctgtccg gcgttccgcg cactgatgac cggcgacagc
     6301 gattttctgg ccacgtccca caatagccac aacgaattgt ggccggctga accagcgaca
     6361 caagacgatg taacggatac gggggataaa ggttttgtcg cccgtatgac ccgcgtaccg
     6421 gaaaatgcag tacagcaggc gctagccggt aaaggtcgca aagtcacgtc actactgaag
     6481 cctgtggcgt ggatgttcgt catcgccgct ctgctgatcg cactcgattc tgcggcaggc
     6541 gtaggggtac tgatactgtt gcagcacggc attgactccg gtgtcgccgc aggcgatatg
     6601 tcgatcatcg gcctctgtgc cctgctcgcc ctgtgcctgg tcattgtggg ctggtgcagt
     6661 tattctctgc agacggtctt cgccgccaga gcggcggaat cagttcagca ttcggtgcgc
     6721 ttgcgcagct tcggccatat gctgcgtctt ggactcccct ggcatgaaaa gcatgccgat
     6781 tcgcgtctta cccgcatgac cgttgatgtg gactctctcg cccgctttct gcaaaacggc
     6841 cttgccggtg cggccaccag tctggtgacg atgttcgcaa tcgccgccac catgttctgg
     6901 ctcgacccgt tcctggcgct gacggcatta agcgcagtgc cagtggccgc actggcaacc
     6961 atgatttatc gccgcctcag tacccctgct tatgcacagg cacggctgga aataggcaaa
     7021 gtcaacagca ccctgcagga aaaagtctct ggcctgcgtg tcgtgcaatc gcatggtcag
     7081 caggaactgg agggcgcccg gctgcgcgcg ttatcggagc gtttccgcgc aacccgtgtg
     7141 cgagcacaaa aataccttgc agtctatttt ccgttcctga cattctgcac cgaggcctcc
     7201 tatgccgctg ttctgttagt gggagcttcg caggtcgccg ctggagaaat gactgccggg
     7261 gtactggcgg ctttcttcct gttgctgggg caattctatg ggccagtgca gcagttatca
     7321 gggattgtcg acgcctggca gcaggcgaca gccagcggca aacatattga tgaactactg
     7381 gcgacagaag gcactgagaa cctcgggtcc tcttcggtcc tccctgtcac cggtgcactg
     7441 catcttgatg aggtcacgtt cagttatccc gacagtcacg agccagctct gaacaaactt
     7501 accctgacga tcccagaggg aatggttgtc gcggtcgtcg gtcgcagcgg tgcgggtaag
     7561 tcgacgctga ttaagctgat tgccgggttg tatttcccca cgcacggcaa catcagaatc
     7621 ggtgtgcaaa tgctcgatga tgcctcgctc actgagtatc gtcgccagat tgggcttgtc
     7681 gatcaggatg tagcactgtt tagtagtgat attgcagaaa acattcgtta ttcacggcca
     7741 tccgccacca atgaagacgt tgaaattgcc tcacagcggg cagggctgta tgagatggtg
     7801 tgcaatctgc cgcagggatt ccggacaccg gtgaataacg gcggagccga tctgcccgca
     7861 ggtcagcgcc agttgattgc gctggcccgc gcgcaactgg cgaatgccca catcctgctg
     7921 ctcgacgaag ccacgtcatg tctggatcgc acatccgaag aacgactgat gtcatcgtta
     7981 acagatgtcg tgcatgccgg gaagcactcg gcgctgattg ttgcacatcg tctgaccacc
     8041 gcgcaacgct gcgatctgat tgccgttatt gataaggggt tacttgcgga atacggaacc
     8101 cacgaacagc tgttatctgc gggcggcctc tatacccgct tatggcatga cagcgtcagc
     8161 agtactgcgc tccatcgcca gcacaacatg aaggaggaaa ccccgggata gttactggac
     8221 acgtaatgta ttaaaaacac agtcagaagc ggcggtaccg tgaatagccg ctttaattat
     8281 ttatactgac atccttaatt tttaaagagt atgaatgctg aacatgcaac aacatccctc
     8341 tgctatcgcc agcctgcgca accaactggc agcgggccac attgctaacc ttactgactt
     8401 ctggcgcgaa gctgagtcgc tgaatgttcc tcttgtgacg ccagtcgaag gagcggaaga
     8461 tgagcgagaa gtgacctttc tgtggcgcgc ccgacatcct ctgcagggcg tttatctgcg
     8521 tctgaaccgg gtgacggata aagagcacgt agaaaaagga atgatgagcg cccttcccga
     8581 aacggatatc tggacactga cactgcgttt acccgcaagt tactgcggct cctattcgct
     8641 gctggaaatc ccccccggca ctacggctga gacgattgca ctgtccggag gccgttttgc
     8701 cacccttgcc ggaaaggccg atccgctaaa caaaatgccg gagatcaacg ttcggggaaa
     8761 cgcaaaggaa tcagtgctga cacttgataa agctcccgcc ctgtcggaat ggaacggcgg
     8821 cttccacacc ggacaactgc ttacctccat gcgcattatc gccgggaaat ctcgccaggt
     8881 tcggctctat attccggatg ttgatatttc tcagcccctc gggctggtcg tgctgcccga
     8941 tggtgaaacc tggtttgatc accttggcgt atgcgcggca attgacgccg ccataaataa
     9001 tgggcgcatc gtgcccgtgg ctgtactggg cattgacaac attaatgaac atgaacgcac
     9061 tgagatactc ggcgggcgca gcaaactgat aaaggatatc gccggacatc tgctgccgat
     9121 gattcgcgct gaacaaccgc agcgtcagtg ggcagaccgt tcgcgcacag tgctggccgg
     9181 gcagagcctc ggcgggatca gtgcgctaat gggggctcgt tacgcaccgg aaacgttcgg
     9241 tctggtgctc agccactctc cttcaatgtg gtggacgcca gaaagaacca gtcgaccagg
     9301 cttgttcagc gaaaccgata cctcatgggt gagtgagcat ctgctttctg ccccaccgca
     9361 gggcgtacgt atcagcctgt gcgtgggatc gctggaaggt tcgacagtgc ctcacgttca
     9421 gcagcttcac cagcggctga ttaccgctgg cgtcgaaagc cattgcgcaa tctacaccgg
     9481 tggtcacgat tacgcatggt ggcgcggtgc actgattgac gggattggtt tactacaggg
     9541 ttgagttgac ccacaaacac tttcaggaaa cggtacagac ttcctgaata aatcaaatag
     9601 tcacctgcgg aaaaggaata atcatcagat gtatgcccgc gagtatcgct caacacgccc
     9661 gcataaagcg attttctttc atctttcttg cctcaccctt atctgtagtg cgcaagttta
     9721 tgcgaagccg gatatgcggc cactggggcc gaatatagcc gataaaggct ccgtgtttta
     9781 ccatttcagc gtcacctctt tcgactctgt cgatggcaca cgccattatc gggtatggac
     9841 ggccgtgccg aatacaaccg caccggcatc gggttacccg attttatata tgcttgacgg
     9901 taacgcagtt atggatcgcc tggatgacga actgctcaaa caattgtcag aaaaaacacc
     9961 gccagtgatc gtggctgtcg ggtatcagac caacctccct ttcgatctca acagcagggc
    10021 ttacgactat acgccagcag cagaaagcag aaaaacagat ctccactcag ggcgttttag
    10081 ccgtaagagt ggtggcagca acaacttccg ccagttactg gaaacgcgta ttgccccaaa
    10141 agtggaacag ggactgaata tcgatcggca acgccgcggc ttatgggggc actcctacgg
    10201 cggcctcttc gtgctggatt cctggctgtc ctcctcttac ttccggtcgt actacagcgc
    10261 cagcccgtcg ttgggcagag gttatgatgc tttgctaagc cgcgttacgg cggttgagcc
    10321 tctgcaattc tgcgccaaac acctggcgat aatggaaggc tcggcgacac agggtgataa
    10381 ccgggaaacg catgctgtcg gggtgctgtc gaaaattcat accaccctca ctatactgaa
    10441 agataaaggc gtcaatgccg tattttggga tttccccaac ctaggacacg ggccgatgtt
    10501 caatgcctcc tttcgccagg cactgttaga tatcagtggt gaaaacgcaa attacacagc
    10561 aggttgtcat gagttaagcc actaaacact gcccgctttt acgcgggcag tacgcctgaa
    10621 acactacgat cagaatgatg cggtaactcc ggcatagtaa gcccggcctg gctcgttata
    10681 ggtattcgcc ccttcagaag atcggaagat ctgtttattg aggatattac tgacgccgac
    10741 attaagacgc agatttttat taatatcgta attgaagttc gtccccacca gtgaataagc
    10801 gcccagctct ttacctgaca gaccgccagt atcttcactg cgggtttccg catgagtacg
    10861 cggtttttgt ctgccatata acgtccagtt gacgctggca gaaaacgcct gggtgatggt
    10921 ccagttaagc gagttattga tagtatattt cgggatgacc gacagaggat taccggtgtc
    10981 tttttgctcc gaagtgatca tccatgtggc attggtattc cagttcagac gatctttcac
    11041 cagtgggaaa gacatactgg cttcgatacc gtccaccaga gctttcccgc cattctgcca
    11101 cttgaggata tatgcgcctg aagcggtttg cccgataacg ttatccccgg ccacgatctt
    11161 attctggtaa tcattgcgga agtaggtcac acttgcgtgg taatcttccc aggtgaactc
    11221 cagcccaatt tctttattga cgctgatttc cggatcgaga tctttattac cgatcaggta
    11281 gcacccgcct gatgtaatat cttttggaca gccattgcct ttcgagtaga gcagatagcc
    11341 ttcactggat tgatacaggt ttggagcttt aaaggttcgg gcaacccctg ctttgacttt
    11401 gaaataatcg cccaattcct gcgaaagatt cagactgggg ctgaagttcc cgccggagtc
    11461 gctgagataa tcaaagcgca ggccgggaat gatattcgtg ccaggaaccg gctcaatgtt
    11521 atcttcaata tacagcgcac tgatttgaga atgattttta ctgctgcgat ccgcagcaga
    11581 gccagaaata ccgctgatat cactgtcatt caccgtcagg ctggtagagg aaggatcatc
    11641 gagcttatcg cggttccact ctgcaccaac ggtcagcgtt tgatcaacca tcacattcaa
    11701 aggaatatta agctcgccgc tggttcgcca ggaactcagg cgattggtcg taaacttttc
    11761 acccgctaaa atacgccctt caccaccgcc ggataatcct tcattcatgc gggtattatt
    11821 ggttttctcg taataaacac caaagcgact ttgtccccag tcccagatac cattatgcgt
    11881 aatgccataa ttctgtcggt acaggcggtt cgtctctttg ccggattttg ccaagctttc
    11941 ggtaactgca ctggaagaac tgttttgcgt atcgcccgca tagatattcc cctggcggct
    12001 atatccggct tcgaaatcga gaatctgctg cggatttaat ttccacgaga caacgccgtt
    12061 aatatctttg ttacgtaccc cttcatgccc ggctgcgttt ttcgtaccga ccggagaatt
    12121 aatatcccaa ctgtcagcat ccgttttatt caggttacca tacaaacgcg tggtaagagc
    12181 attaccagcc agaggcccac taaggctgaa attggcgcga cgcgtagcgc cctcatcgct
    12241 actttccggc tgattggtgt ataacgacag cgaaccgtgc cagtcgttgg tgggacgttt
    12301 ggtaatgatg ttcaccaccc ccccggctgc ccccgaaccg tagcgcgccg ccgcagggcc
    12361 gcggatcact tcaatacgct caacctgttc cggtggcacc cagttggtgt caccgcgggt
    12421 atcacgctct ccacgccagc tataacgcac ggagttacgt gacgtcaccg gtacaccatc
    12481 aattaaaatt aaggtgtttt ccggccccat accacgaata tcgatctggc ggttgttacc
    12541 gcgtgtgccc gaggcgctat tgccggtaag attgacgcca ggcattttac gaataatatc
    12601 tgaaaggtcg tttaccggag gggtcttttt aatatcctcg ctggtaataa ccgacacgcc
    12661 cggctgctgt tttaatacct gctcagcggt ggcttccacc accagagtct cgtcattatc
    12721 atcgtcggag gatttggcta ctgatacctg gctattcaac ccaaccagga gcacagttag
    12781 cgaccagagg attttgttaa ttctcatacc tattccctaa taaatgccta acttaaaatg
    12841 tttgatcgtt aagctcacat ccttgccaga tattttttac tgccattatt gtttttatat
    12901 aagaatgata attaatatca tttagcaaaa aaaaaagcaa tccctcacaa gataaatata
    12961 tt
//
LOCUS       DAJOWB010000126        12955 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJOWB010000126 DAJOWB010000000
VERSION     DAJOWB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.126"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            347..1045
                     /locus_tag="OHO85_003910"
     CDS             347..1045
                     /locus_tag="OHO85_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673220.1"
                     /translation="MSIESKEGSISQRTINLNEYETAINIINEHVTKENIHNTVQSLT
                     EKDISKINSSDKHHKISSEESIKSQLYSDQKKYADLLLHSEKNTEWYKYASSEERYDK
                     FKNSSKEIKNTYKQIVLAQKKLNQMKYINKLGGELIDIADKKLAPLINDSFSYTRDFF
                     AYSKQENNIFTFDNSKFVDPKEKEGLMIQHSNGQLVITGKYCPEGVQTAFTQEQYDKL
                     IRHINIFFTFPKCE"
     gene            complement(1264..2340)
                     /gene="glpQ"
                     /locus_tag="OHO85_003911"
     CDS             complement(1264..2340)
                     /gene="glpQ"
                     /locus_tag="OHO85_003911"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCQ2673221.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(2345..3703)
                     /gene="glpT"
                     /locus_tag="OHO85_003912"
     CDS             complement(2345..3703)
                     /gene="glpT"
                     /locus_tag="OHO85_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCQ2673222.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            3976..5604
                     /gene="glpA"
                     /locus_tag="OHO85_003913"
     CDS             3976..5604
                     /gene="glpA"
                     /locus_tag="OHO85_003913"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCQ2673223.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVYGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            5594..6853
                     /gene="glpB"
                     /locus_tag="OHO85_003914"
     CDS             5594..6853
                     /gene="glpB"
                     /locus_tag="OHO85_003914"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCQ2673224.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDQTLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            6850..8040
                     /gene="glpC"
                     /locus_tag="OHO85_003915"
     CDS             6850..8040
                     /gene="glpC"
                     /locus_tag="OHO85_003915"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCQ2673225.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLTKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            8234..9136
                     /locus_tag="OHO85_003916"
     CDS             8234..9136
                     /locus_tag="OHO85_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCQ2673226.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(9177..9647)
                     /locus_tag="OHO85_003917"
     CDS             complement(9177..9647)
                     /locus_tag="OHO85_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCQ2673227.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(9702..10904)
                     /locus_tag="OHO85_003918"
     CDS             complement(9702..10904)
                     /locus_tag="OHO85_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCQ2673228.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            complement(11004..11546)
                     /locus_tag="OHO85_003919"
     CDS             complement(11004..11546)
                     /locus_tag="OHO85_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673229.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            11825..12250
                     /gene="nudI"
                     /locus_tag="OHO85_003920"
     CDS             11825..12250
                     /gene="nudI"
                     /locus_tag="OHO85_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCQ2673230.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(12289..12891)
                     /locus_tag="OHO85_003921"
     CDS             complement(12289..12891)
                     /locus_tag="OHO85_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCQ2673231.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGMTVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMNVEKGKVYLDGEFVNH"
BASE COUNT         3438 a         3376 c         3074 g         3067 t
ORIGIN      
        1 tcgctgtaat attttacaaa ataataaatt gcaaccagaa gaagtttaca aagaatcaca
       61 acaaaccgcg aaacttgaga ttttttgtga tgagtttcta aaaatatctc aaagtagata
      121 cggattatca acctcagcag acagcatagc taatttattg acttttttca cgaaagcctc
      181 agatgcaatt gatagaatca agacccaaaa aattgacgtt cacagttatg gtttcgtacc
      241 aataagacat tttgtcgaac atgtcataac gtataaaaat gaatttgcag cggattatga
      301 accctatacc cttaccttta cccgaggtga aaacaacgaa ggtgttttga gtatcgaaag
      361 caaagaaggg tctatttcac aaagaacaat aaacttaaat gaatatgaaa ctgcaattaa
      421 catcatcaat gaacatgtta caaaagaaaa tatccacaac acggtccagt cactgacaga
      481 aaaagacatt tcaaaaataa acagctctga taaacatcat aagatcagca gtgaggaaag
      541 tattaaaagt caactttaca gcgatcaaaa aaaatatgca gacctgctac tacattctga
      601 gaaaaataca gaatggtaca aatacgcatc ctccgaggaa cgctatgata aattcaagaa
      661 ttcttctaag gaaattaaaa acacctacaa gcaaattgta ctagcgcaaa aaaaattgaa
      721 ccaaatgaaa tacatcaata aattaggtgg tgagcttatc gatatcgccg acaaaaagtt
      781 agcaccatta ataaatgata gtttcagcta tacacgagat ttctttgctt actcaaaaca
      841 ggaaaataac atcttcacat ttgataattc taagtttgta gatccaaagg aaaaagaagg
      901 tttaatgata caacacagca atgggcaact ggttattact ggtaaatatt gccctgaagg
      961 tgtgcaaaca gcattcaccc aagaacaata tgataaatta ataagacaca ttaatatttt
     1021 ctttactttt ccgaaatgcg aataatactt tacagtataa actgcaataa aattaattat
     1081 tgaacccaat tcatactgtt taaagaataa caatacccag tgttagcttc cctaacgaat
     1141 gagttttacg caaatatgtt ggcatttttt taacaggatg caccggttaa tgaatagaac
     1201 aacggtagta gcattatttc gctttagcag aggatggcgc atgaacgcca tcctgcaata
     1261 gcattactct ttattaagga attttactgc cttatcaggg aaatcagtaa acagcccatt
     1321 tacacccgct ttgttataca gagcatcata taactgattc acatcagtag tgtattcagg
     1381 cagtttatcc gaccgcacgg tataaggatg cactaccagt ttattctgct gagcatcttg
     1441 caccatgcca gtgagtttga tattacccgg ctgcgatgtt tcctcaatca acatatggta
     1501 atccggacca ataccatctg catattccgc cacctgtttc atggcacccg gcttaaacat
     1561 ccagtcgtag ttgtaattaa cccagcttcc atccggctgt ttctgctgcg tttcattcca
     1621 gtcggtatag gcaatcagct gtaccagatt gagctccatg cccattttgg gttccagctc
     1681 attcttaata cgcttcagct catcagcatc aaaacattgc aaataaactt tatcgtcttt
     1741 accggtgtaa ccatatttct tcagcacttc cagcgttttt gccgcaatat ccttcccttc
     1801 ctgatgatgg aaccacggcg ctttgatttc tggataaata ccgatatttt tcccggtaga
     1861 gtgatttaac ccctgaacaa attcaatctc ttcttcaaag gtgtgcaccc ggaagtcgga
     1921 cttacccatt gggaaacgcc ccggataagt ctgcactttt ttaccgtttt caatatcgaa
     1981 accttcggta aatttcagcg acttaatttc atccagcgtg aaatctatcg cgtagtaacg
     2041 accgtctttg cgcgcccgat ccgggaaacg atcggcaaca tcagtaacac gatcgagata
     2101 atggtcatgc agaacaacca gatggtcgtc tttggtcatc accaaatcct gttccagata
     2161 atccgctccc tgcgcatacg ccatcgcttt tgctggcagc gtatgctccg gcaaatatcc
     2221 actggcaccg cgatgggcga tgactatttt ttcgttgctg tccgccgcca ttgcactgct
     2281 tcccatgact atagtgctca tcatgatcgc catgctaagg tttttcagcg tcaatttcat
     2341 gccattagcc tccgttgcgt ttttgcagta attgttcatg gcgacgtttt tcgccaatca
     2401 tcacaacaat caacaagata accgccagaa tgctgccgcc aatcattacc ataaagccgc
     2461 catcccagcc gaagaagtcc acggtgtagc caacaatcgc gctcgccgcc accgaaccgc
     2521 ccaggtaacc aaacagcccg gtaaagcccg ctgccgtacc tgccgctttt ttcggtgcca
     2581 gttccagcgc atgcagaccg atcagcatca caggaccgta gatcaggaag ccgataacaa
     2641 tcatacaaat catatcgacg gttgggttac ctgccgggtt catccagtaa acgatagtcg
     2701 cgatggtcac cagtgtcata aagaaaacgc cggttgcccc acggttgcca cggaagactt
     2761 tatccgacat ccagccgcac agcagagtgc ccggaatacc tgcatattca taaaggaagt
     2821 aggcccagga ggatttatcc agcgcgaaat gcttaacctc tttcagataa gtcggtgacc
     2881 agtcgaggat gccgtaacgc agcagataaa cgaacacgtt ggcgatggcg atataccaca
     2941 gcagtttgtt cggcagtacg tactgcatga agatttgctt cgccgtcagc tcctgttccg
     3001 ctttttcgtt atagtcgtcc ggataatcat ttttgtactc ttcgatcggc ggcaagccac
     3061 aggattgcgg ggtatcgcgc atcatcgcaa aggcgaataa tgccaccaga atggcgcaga
     3121 aagcgggcat atagagcgcc gcgtgccagt cattgaacca ggccatcccc agcaggaaca
     3181 gcagcggcgg aataccacca ccaacgttgt gcgcacagtt ccacactgac acaatgccgc
     3241 cacgttcttt ctgcgaccac cagtgcacca tagtacgacc acacggcggc caccccatcc
     3301 cctggaacca accgcagagg aacaaaagta caaacatcac cgcaatgctc gacgtcgccc
     3361 atggcacaaa gcccataaac aacatcactg ccgccgccag aatcaaacct gcggggagga
     3421 aaacgcgcgg attcgagcga tctgataccg aacccattat gaatttcgaa aatccataag
     3481 cgatcgagat ccccgaaagc gcgaaaccta aatcaccgcg agagaatccc tgctcgacca
     3541 gataaggcat agcaagcgca aagttcttac gaaccaaata gtaagccgca tagccaaaga
     3601 atatccccag gaaaatttgc cagcgcaatc gacgataagt cggatcgatc tccgcagcag
     3661 gtaagcgcgc tttgtgtggc gctggtttaa aaatactcaa cattgaaagc ctccgtggcc
     3721 cgtggtctta tttatgatta acagactgat tcagtgggag aacctgccgt ttctttagtt
     3781 gccgcgatgt taagaaaaca ttcagaaatt aaatgtgaat tgccgcacac attattaaat
     3841 aagatttaca aaatgttcag aatgacgcat gaaatcacgt ttcactttcg aattatgagc
     3901 gaatatgcgc gaaatcaaac aattcatgtt tttactatgg ctaaatggta aaaaacgaac
     3961 ttcagaggga taacaatgaa aactcgcgac tcgcaatcaa gtgacgtgat tatcattggt
     4021 ggcggcgcaa cgggagccgg gattgcccgc gactgtgccc tgcgcgggct gcgcgtgatt
     4081 ttggttgagc gccacgacat cgcaaccggt gccaccgggc gtaaccacgg cctgctgcac
     4141 agcggtgcgc gctatgcggt aaccgatgcg gaatcggccc gcgaatgcat tagtgaaaac
     4201 cagatcctga aacgcattgc acgtcactgc gttgaaccaa ccaacggcct gtttatcacc
     4261 ctgccggaag atgacctctc attccaggcc acttttattc gcgcctgcga agaagcaggg
     4321 atcagcgcag aagctataga cccgcagcaa gcgcgcatta tcgaacctgc cgttaacccg
     4381 gcactgattg gcgcggtgaa agttccggat ggcaccgttg atccatttcg tctgaccgca
     4441 gcaaacatgc tggatgccaa agaacacggt gccgttatcc ttaccgctca tgaagtcacg
     4501 gggctgattc gtgaaggcgc gacggtgtac ggtgttcgtg tacgtaacca tctcaccggc
     4561 gaaactcagg cccttcatgc acctgtcgtg gttaatgccg ctgggatctg ggggcaacac
     4621 attgccgaat atgccgatct gcgcattcgc atgttcccgg cgaaaggatc gctgctgatc
     4681 atggatcacc gcattaacca gcatgtgatc aaccgctgcc gtaaaccttc cgacgccgat
     4741 attctggtgc ctggcgatac catttcgctg attggcacca cctctttacg tattgattac
     4801 aacgagattg acgataatcg agtgacggca gaagaggttg atattctgct gcgtgaaggg
     4861 gaaaaactgg cccccgtgat ggcgaaaacg cgcattttgc gggcctattc tggcgtgcgc
     4921 ccactggttg ccagcgatga cgacccgagc ggacgtaacg tcagccgtgg catcgtgctg
     4981 ctcgaccatg ctgaacgcga tggtctggac ggatttatca ccatcaccgg tggcaaactg
     5041 atgacctatc ggctgatggc tgaatgggct accgacgcgg tatgccgcaa actgggcaac
     5101 acgcgcccct gtacgactgc cgatctggca ctgcctggtt cacaagaacc cgctgaagtt
     5161 accttgcgta aagtcatctc cctgcctgcc ccgctgcgcg gttctgcggt ttatcgtcat
     5221 ggcgatcgca cgcctgcctg gctgagcgaa ggccgtctgc atcgtagcct ggtatgtgag
     5281 tgcgaagcgg taactgcggg tgaagttcag tacgcggtag aaaatttaaa cgttaatagc
     5341 ctgctggatt tacgccgtcg tacccgtgtg gggatgggta cctgccaggg cgaactctgc
     5401 gcctgccgcg ctgccggact gctgcaacgt tttaacgtca cgacgtccgc gcaatctatc
     5461 gagcaacttt ccaccttcct taacgaacgc tggaaaggcg tgcaacccat cgcctgggga
     5521 gatgcactgc gcgaaagcga atttacccgc tgggtttatc agggattgtg tggtctggag
     5581 aaggagcaga aagatgcgct ttgatactgt cattatgggc ggcggcctcg ccggattact
     5641 ctgtggcctg caactgcaaa aacacggcct gcgctgtgcc attgtcacgc gtggtcaaag
     5701 cgcgctgcat ttctcatccg gttcgctgga tttgctgagc catctgcctg atggtcaacc
     5761 ggtgacagac attcacagtg gactggaatc tttgcgtcag caggcaccag cccatcctta
     5821 cagtcttctc gaaccacaac gcgtgctcga tctcgcttgc caggcgcagg cattgatcgc
     5881 tgaaagcggt gcgcaattgc agggcagcgt ggaactggcc catcagcgcg ttaccccgct
     5941 cggcactttg cgtgcaacct ggttaagttc gccagaagtt cccgtctggc cgctacccgc
     6001 gaagaagata tgtgtagtgg ggataagcgg gctgatggat ttccaggcgc atcttgcggc
     6061 ggcttcgttg cgcgaactgg atctttcggt tgaaaccgca gaaatagagc tgccggaact
     6121 ggatgtcctg cgcaataacg ccaccgaatt tcgcgcggtg aatatcgccc gtttccttga
     6181 taatgaagaa aactggccgc tgttacttga tgcgcttatt cctgtcgcca atacctgcga
     6241 aatgatcctg atgcccgcct gcttcggcct ggccgatgac aaactgtggc gttggttgaa
     6301 tgaaaaacta ccttgttcac tgatgctttt gccaacgctg ccgccttccg tgctgggcat
     6361 tcgtctgcaa aaccagttac agcgccagtt tgtgcgccag ggtggcgtgt ggatgccggg
     6421 cgatgaagtg aaaaaagtga cctgtaaaaa tggcgtagtg aatgaaatct ggacccgcaa
     6481 tcacgccgat attccgctac gtccacgttt cgcggttctc gccagcggca gtttctttag
     6541 tggcggactg gtagcggaac gtaacggcat tcgcgagccg attctcggcc ttgatgtgct
     6601 acaaaccgcc acgcggggtg aatggtataa gggcgatttt tttgcgccgc aaccgtggca
     6661 gcagttcggt gtaaccactg atcagacgct acgcccgtcg caggcagggc aaaccattga
     6721 gaacctgttt gccatcggtt cggtgctggg cggatttgat cccatcgccc agggatgcgg
     6781 cggcggtgtt tgtgccgtca gtgctttaca tgccgctcaa cagattgccc aacgcgcagg
     6841 aggccaacaa tgaacgacac cagcttcgaa aactgcatta agtgcaccgt ctgcaccacc
     6901 gcctgcccgg tgagccgggt gaatcccggt tatccagggc caaaacaagc cgggccggat
     6961 ggcgagcgtc tgcgtttgaa agatggcgca ctgtatgacg aggcgctgaa atattgcatc
     7021 aactgcaaac gttgtgaagt cgcctgcccg tccgatgtga agattggcga tattatccag
     7081 cgcgcgcggg caaaatatga caccacgcgc ccgtcgctgc gtaattttgt gttgagtcat
     7141 accgacctga tgggtagcgt ttccacgccg ttcgcaccaa tcgtcaacac cgctacctcg
     7201 ctgaaaccgg tgcggcagct gcttgatgcg gcgttaaaaa tcgatcatcg ccgcacgcta
     7261 ccgaaatact ccttcggtac gtttcgtcgc tggtatcgca gcatagcggc tcagcaagca
     7321 caatataaag accaggtcgc tttctttcac ggctgcttcg ttaactacaa ccatccgcag
     7381 ttaggtaaag atttaactaa agtgctcaac gcaatgggta ccggtgtaca actgctcagc
     7441 aaagaaaaat gctgcggcgt accgctaatc gccaacggct ttaccgataa agcacgcaaa
     7501 caggcaatta cgaatgtaga gtcgatccgc gaagctgtgg gagtaaaagg cattccggtg
     7561 attgccacct cctcaacctg tacatttgcc ctgcgcgacg aatacccgga agtgctgaat
     7621 gtcgacaaca aaggcttgcg cgatcatatc gaactggcaa cccgctggct gtggcgcaag
     7681 ctggacgaag gcaaaacgtt accgctgaaa ccgctgccgc tgaaagtggt ttatcacact
     7741 ccgtgccata tggaaaaaat gggctggacg ctctacaccc tggagctgtt gcgtaaaatc
     7801 ccggggcttg agttaacggt gctggattcc cagtgctgcg gtattgcggg tacttacggt
     7861 ttcaaaaaag agaactaccc cacctcacaa gccatcggtg cgccactgtt ccgccagata
     7921 gaagagagcg gcgcggatct ggtcatcacc gactgcgaaa cctgcaaatg gcagattgag
     7981 atgtccacga gccttcgctg cgaacatccg attacgctac tggcccaggc gctggcttaa
     8041 ccgacctttt gatgcccggt aagcacattg cttatcgggc atttttgcga gcgcgattcc
     8101 acgcaccatg tatgcgttgc aacgctttga aaattcctct gaaaatgtct cgcaaaggct
     8161 gaaactgaca gatgtcaaag gcccgggata gacgtaatgt cgcgtcatca taaatatcag
     8221 gtgacggaca accatgaccg aatcaacaac atcctccccg catgatgcgg tgtttaaaac
     8281 ctttatgttc acacccgaaa ccgcacggga ttttctcgaa atacatttac cagaaccact
     8341 gcgcaagctt tgcaacctgc aaaccttacg cctggaaccc actagtttta ttgaaaaaag
     8401 tttacgcgct tactactcgg atgttttgtg gtccgtggaa accagcgacg gtgacggcta
     8461 tatctactgc gtgattgaac atcaaagctc tgcagaaaag aatatggctt ttcggctaat
     8521 gcgctatgcc actgccgcca tgcagcgtca cctggataaa ggctatgaca gagttccgct
     8581 ggtggtgcca ttgctgtttt atcatggcga aacatcgccc tacccgtact cacttaactg
     8641 gctggatgag tttgacgatc cgcaacttgc ccggcagttg tacaccgaag cttttccgtt
     8701 ggtggatatc accatcgtac ctgacgatga gatcatgcaa catcggcgta tagctctgct
     8761 ggaactgatt caaaagcata ttcgcgaccg cgatttaatc ggcatggtcg acaggatcac
     8821 cacgcttttg gttagaggct tcactaatga cagccagcta caaacactgt ttaattatct
     8881 gctgcaatgc ggcgatacct cccgtttcac ccgttttatt gaggagattg ccgaacgttc
     8941 accactacaa aaggagagat taatgactat tgctgaacgg ctacggcagg aagggcatca
     9001 aattggctgg caggaaggta tgcatgaaca agccattaaa attgctttgc gcatgctgga
     9061 acagggcatt gatcgtgacc aggtgctcgc ggccacccag ctaagcgaag ccgatctggc
     9121 agcgaataac cactaattaa cacaggccca cagccgatcc ccatgggcct ttgatatcaa
     9181 taactacctt ttatgcgtgg cccatttttg cctgatttaa acatcgccag tcgttgatcc
     9241 agggcatcgc tgtagagcat cgtgtcaacg ccaacagcga caaagttcgc tccccacgcc
     9301 aggcattgct gcgccatatc aggagccaca gccagaaaac cagccgcttt acccgcagca
     9361 cggatccgcc gaatgctggt ttcaataatt cgctgcactt ccgggtgccc ggcgttatcc
     9421 gggtagccca acgacgcaga aagatccgct ggtccaataa acacgccatc aatcccttcg
     9481 acgtcgagga tctcgtccag gttatccagt gccgttttac tttccacctg cactaacaga
     9541 caaagcgaat cgttgacttg cgccatgtaa ttctcaatgc gtccccagcg cgcagcccgt
     9601 gccacactgg ccccaacacc acgctcaccg tagggagggt tggacaagtt ttgccggagg
     9661 gggaatgtgc cggtgcagta aaccggcaca gagtggcgga atcagacaga taaggtcatg
     9721 gactcaataa cctcaatcca gccatgctca ctggcgatat cctggccgtt taaccaacgg
     9781 cgcagcatat tcagtgccat cattgcgcac acttcctgac ggatagccag gctgtagcgc
     9841 gtagtgctga aacgcacacg cagagcgaaa gtgccgtctg gcgtggctag cgcaaagttg
     9901 agatgctcgt tctcgaaacc cgaaacagcc agtgccagcc cggcaaaatg gttggcccgc
     9961 cgttctgtaa tccagtgcgc agtttgcgcc agggtttctt cctgtgaagg aaccacttca
    10021 cacgccagca atggagcacc tgcgcgagaa agttgcaaag ccaataaacc accggtgaat
    10081 tgctcgctca acgtcaggct gaactggcgg ttttgcaatt cgcgactgat ctgcgcgggc
    10141 agtccttcgg tgccttcgaa aatcacgctc tgtccggcaa cacgtttaac gtccagccac
    10201 agtttttcca tcgcctgttg ctcgcttgcc ggtccggtga gtttcagttc gatgatcggc
    10261 attgaggagc gatagcccat tgttacgccc ggcggcagtt gtagagtgtc caggctttgt
    10321 gccagatcgc tttccgaacg accaaaggta gttaaacgca gacaaaccgg cggctgtggt
    10381 aaagaaaaac gctcgcgcag gcgcggcagg atttcgtgct cgaccatcac cttaaattct
    10441 gacggtacgc cgggagtaaa gaacatcagg caacgattaa gctgcacggc aaaaccacag
    10501 gcggtgccta ccgggttatt gataaattca gcactggcag gcagctccgc ttgtttacgg
    10561 ttgctcggtg ccattactcg tccacgttcg tggaaatagc gttccatctc tttgagccag
    10621 gcttcatgca gcaccaggcc ttcacctttt gctgtcgcag cggcgagtgc gcttaaatca
    10681 tcgctggtcg gtcccagccc gccgttaacg atcagcacat cggcgtgctg actacgttcg
    10741 cgaagaatgg tgactaagtc atcaaggtta tcccccaccg tattgcggcg agataatggc
    10801 agcccctgat gaaagaaaaa atcggccagc caggcagcgt tagtgtcaac gatttgcccg
    10861 tgtaacactt catccccggt ggataacatt tccactttta acatggttat ctcctgcttt
    10921 tgttatcgaa tcactataac gcaaagggag ataaggcgta gtaagaaact gaatggcgcg
    10981 acagaatgcc gcgccggaag ggatcagaaa ctggcgttaa ccccaacata cgggccgtca
    11041 gccacggcgt tgtcgcggtt accgtcttta cccgacagat tcaggtagcg ataacccgcc
    11101 tcaatactga ctggacgcat aatggtgtaa cgcgcgccag cattcgcttc ctcataactt
    11161 tgaataccgc tggagagcga atccggagag tagtaatact cgccaaacaa acggaagctg
    11221 ttgccaattt tccactgcaa accacctcct accgctgcgg cataaccttc atcgccgtaa
    11281 ttcgggttgg tgtaaacgcc ttttccgcca acggtcgcca ttaacggccc gagaggcaga
    11341 ttcaacccca gccccacgcc cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg
    11401 cttaaagcca ggcccgtcga ttcagtccca aaaccgacac caatattggt gtattcttta
    11461 cccgcctggc cgctgatgct cattgcattg accgatgcag aaaccaacag cattccggcc
    11521 agacctgcta gcgcaatttt tttcattatc ccctttccca caagcaatta atcaaaattc
    11581 ccaaagctgc gggattgtac tgcctggcac agaggattca atgagcgaaa aaagggatct
    11641 agtcacttat tgtaactaaa tacttcttaa ataattaaac gccgaaaaaa taaacacact
    11701 ttaccgatac acacaaaaat aataataagt gcagttatca ttgcaaaaga gattactgtc
    11761 actttcacat atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac
    11821 ccctatgcga caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct
    11881 gtgtaaaatg gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg
    11941 cgtggagtct ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg
    12001 agaacagctg cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa
    12061 gacgtatgca gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt
    12121 ttctgccaac cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc
    12181 tgaagatctg gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg
    12241 tcttctttaa caatgatgac agccatcact ggctgtcaga ttgaaaattt agtggttaac
    12301 gaattcccca tccagataaa ctttgccttt ttccacattc atgactaaac catccagata
    12361 atcaggttta aatgtcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg
    12421 ggtgaaaata acgatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata
    12481 aatctcatta ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa
    12541 ccaggtcgcc gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat
    12601 gtcagcgcta aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtcatacc
    12661 tgttttatct gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa
    12721 aacgacaacc gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa
    12781 accattgcta ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa
    12841 aatgatgaaa tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc
    12901 acctatagac aagcgcaacc agacaattac cgtaaaattg agcgacattt ctggc
//
LOCUS       DAJOWB010000127        12669 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJOWB010000127 DAJOWB010000000
VERSION     DAJOWB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.127"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(137..1591)
                     /gene="amn"
                     /locus_tag="OHO85_003922"
     CDS             complement(137..1591)
                     /gene="amn"
                     /locus_tag="OHO85_003922"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCQ2673232.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            complement(1693..3009)
                     /gene="shiA"
                     /locus_tag="OHO85_003923"
     CDS             complement(1693..3009)
                     /gene="shiA"
                     /locus_tag="OHO85_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HCQ2673233.1"
                     /translation="MDSTLISTRPDERTVSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAMYLLAGCLISAITALLMKDSQRA"
     gene            3324..4376
                     /locus_tag="OHO85_003924"
     CDS             3324..4376
                     /locus_tag="OHO85_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HCQ2673234.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNVLKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVDLTMKCYSPVCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            complement(4638..12011)
                     /locus_tag="OHO85_003925"
                     /pseudo
     CDS             complement(4638..12011)
                     /locus_tag="OHO85_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
BASE COUNT         3176 a         3015 c         3054 g         3424 t
ORIGIN      
        1 atgtggaaat cattaagacg ataataaaac tcgtatccgc ccaaggaagc aaacagcccg
       61 cgtttattgc gggctatttg ccagatgtct tacttaatcc ttcacatcta tttttgataa
      121 gaaggttcat aacttattat cggaacggcg gctcattaaa ggttcgtaat ttacgtgaat
      181 gcagtcggtc gccttctgcg cgcagcaaat cgatcgcccg aatgccaatt tgcaggtgtt
      241 cggaaatagc accttcataa aaacggttag cctggccggg aagtttaatc tcgccatgca
      301 acggtttatc tgaaacacac agtagtgtcc cgtatggcac gcggaaacga tatccttgcg
      361 cggcaatggt tgcactttcc atatcaattg ctacggcccg gcttaagtta aaacgaagtg
      421 cagaagctga gtaacgtaat tcccagttcc tgtcatcagt ggttactaca gtgccagtac
      481 gtagccgctg tttaacttcc tcaccgggcc tgccgctcac cagcttggtg gcgtcataaa
      541 gcgcacgttg cacttcagca atgctcggaa taggaatatc gggcggcaga accgcatcaa
      601 gaacgtggtc atcgcgtaaa taagcgtgtg caagtacata atcgccaatg gcctgacttt
      661 cacgtaatcc gccacagtga ccaatcatca accagacatc cgggcgtagt actgccagat
      721 gatcgcagat ggttttagca tttgacggtc ccacgccaat attcaccaga gtaataccct
      781 gaccatcggc ggtaattaaa tgccacgcgg gcatctggtg ttttttccat gcaaggtcgg
      841 aaatggcttc ttctggcgct tcggtttcgg cggtgatcca gttcccgcca gcacaagaaa
      901 gggcaatgta gggactatca ggatcgagga tctggctgca tccccaacga acgaattcat
      961 ccacataacg tgtgtagttg gtaaacaaga cgaacggctg aaaatgttca actggcgtac
     1021 cggtataatg gcgcaaccgc gcgagggaaa aatcgacgcg gcgcgcatca aaatgcgata
     1081 gcggggagaa ttcagttggg tgataaatgc cgtctgcagt ttcatcgcca atttgcgcca
     1141 gttctgttgt agggaaatag cgagttaacc cagcgctcat tgagcgatca agtgtcaatt
     1201 cagagccatc gatgacataa ggataaggga tttcatgctg cgagggttgc accgagatat
     1261 gcgcaccata atcctgatac agcaacgtaa gttgttcatt aagatacgaa cgaaagagag
     1321 tagggcgagt aatcgtggtg gtgtagctgc ctgcgtgagt aaaacgacca aatgcgcgcg
     1381 ttttgggggg attggttgtg ctaccgtccc aggttacggt cagtgatgga tagacaaaaa
     1441 gaccttgttt gcgggcgttt tcatcaggta attcgccact tgtaatatag ttgccaatgg
     1501 cgttgcgtaa tgcgactaca gattgctcat acagcgcgtc gagtttatcc agtgcctgag
     1561 ctggggtcag accggagccc ttattattca tgtgtgtctc ctgttccata caattaccgc
     1621 acatccgata gtatgtcaca gaaatgtgaa acaaaagtgt aaggcgaaga tcggatgatc
     1681 ttcgccaggc tatcaagcgc gttgactgtc tttcatcaac aaagcggtta ttgcagaaat
     1741 cagacatcca gccagcaaat acatggcgac gctatgccag ttcccggcaa agtaagtgat
     1801 gagtgcagcg gcaataaaag gtgtaaatcc tccgccaacc acactggcaa cctgataacc
     1861 gactccagcg ccactatagc gataactggc accaaacatt tcggtaaaca tcggttgttg
     1921 cacacacacc accatgtcat gcgcaatgtt tgccagcatt atggagaaga aaactatcca
     1981 gaaaatagat tgtgcttcaa gcgccataaa gaaaggaaat gcgctcaacg ttccgattaa
     2041 cgcacctgtg atataaaccc tgcggcgacc aaaacgatcg gcaagccagg caaaacaggg
     2101 aattgtcagg cagcttaatc cacctaccag caaaccaata ttaaggaaaa gttcgcgcgg
     2161 taaccccata ttctgggttg aataattaag tgcaaaggcg gtaacgatgt acatcgtcag
     2221 caactcgcac agccgtagcg caataatctt caggaaagca cctggatgtc gtaacagcgc
     2281 ttcgataacc gggatgcgtt ttttcgcggc agcttgatta tgttgctgtt gttcgaattc
     2341 cgcggactcc tccatgccat tgcgcaccca caatgctccc agtaccagta cgatgctaaa
     2401 caggaaagga atacgccagc cccaacttaa aaactgttcg tcagtcgtca tcatactgat
     2461 caatgaaacc agtccggttg aaagcagtaa acctacaccg tagccaactt gtacaccgct
     2521 actgtaaaag gcttttttat ttttcggtgc actttcaacg gaaagcaacg ccgcgcctcc
     2581 ccattcgccg ccgacggcaa atccctgaat ggcacgcagc gtcaccagta aaataggtgc
     2641 ccaccaccca atggtcgaga atgaaggaag aataccaatc aaggctgtcg cgatgcccat
     2701 catccagaca gtcagcatta acatgcgctt acgtcccagt cggtcgccaa agtgaccgaa
     2761 aatgacaccg ccgagcggac ggaaaaggaa tccgacgcca aaggtagcaa atgcggcgag
     2821 cgttcccatc gccgggctta cttgcgggaa aaactcgcga ttaaacacca gtgcggcggt
     2881 gatgccatag agtaaaaaat cataccagtc gacgacggca ccagcgaaac tgcctaacgc
     2941 agctcgtcgg gcgcgactta acgaaaccgt cctttcatcg ggacgagtgg agatgagcgt
     3001 ggagtccata atgatcctgt ttgtactgtc tttttataat tagtatggaa aaggtaactc
     3061 acattgccgt cgatctataa acgaacctta tgagactacc tcgacagagc gcatctgcaa
     3121 atgattttat ggctgattag cggcaacatg aaaaattcgc tggaagatga aagcgcggga
     3181 gttgctggtg ttgtttaagg attaagcaga ggacccacag gttcgagata tgaaacataa
     3241 aagtccgcat atgcacaaaa tggcaccctg atgattctga tagactgcat tgcattataa
     3301 ccagtaggga ggcgaggtta gttatgtttt tagtatcatt gttgagacgt attgcattta
     3361 gctactacga ttataaagct tataatttca atattgaaaa aacagacttt gttgtcatcc
     3421 atattcccga tcagattggc gatgctatgg ccatctttcc tgttattcgg gcgcttgaat
     3481 tgcataaaat taagcatcta ttaattgtta cgtcgacaat taatttagaa gtctttaatg
     3541 tgctcaaact tgaacagact aaattaacat tagtcacaat gactatgcag gatcacgcaa
     3601 cattaaaaga aataaaagat ttagcgaaga acataacaca gcaatacggt acgccggatc
     3661 tttgtataga ggcgatgcgt aaaaagaacc tgaaaacgat gttatttatc agtcagttga
     3721 aagcaaaaac gaattttcag gttgttgatt taaccatgaa gtgctattcc cctgtgtgta
     3781 agaacgcgtc ccggatggat cagaatctcc gggctcccgt acccatgaca tgggcattta
     3841 tgatgcgtga ggcgggtttt ccagcagtca ggccaatata tgaattgcca ctaagtgagg
     3901 atgtactcga tgaggtgcgc gaggaaatgc gatcgttagg atcttacatt gcgcttaatt
     3961 tagaaggtag ctcgcaggaa cgtacatttt cactatcgat tgcagaaaat ctaatagcaa
     4021 aaattcaaag tgaaacagat ataccaatag tgatcgttca tggaccaaaa ggggaagata
     4081 aagccagggt attagtggat tgttataata atgtctaccg tttatcctta tcaccctcga
     4141 ttaaacgttc agcagcaatc ataaaagatg cttatatcgc aataactcct gacacttcaa
     4201 tattacatat ggcaagtgcc tataataccc ccgttgttgc aatttatgct gattacaaaa
     4261 cgcgatggcc cgcaatggcc gatgtttcgg agtcagtcgt cgttgggcaa aaaattgaca
     4321 atataagtct ggatgaattc gcaaaggcat taaaaagtgt tttggcgaga atatgagaca
     4381 gtacttatgc atagtaatgc cgatctgtta tgaacggtgg tgtcatcaat actctgaggc
     4441 tggtcaatca tcacagaaaa ccagcctttg gatcaaagct gtactcaccg gatacgatca
     4501 tagtcacacc gaacgcgaat ctggcggtat cgcattttta tttattcatt ttcggtgaga
     4561 gaagagcaag ctctatagag ggaatgtaaa aaagagtgcc ccgaatggag cactctttaa
     4621 gaaattataa cggaaaatca gaggttttta taacatgtcg cataagcaaa cccaagcttt
     4681 tcaaatacgc tttgatcacc tgttgccagg gaaactacgt attgttcgtt tgagtttgcg
     4741 ggactagatg accaatacat atttgattga aaaccagcct cagtcgtgta atgccccata
     4801 tctccccatt cactgtacag tgacccgatg cctcgggaac gactatggtt taaatgatct
     4861 gcacggggaa gtgtgtagcc attgctactg caaaaatttt cagcaaggct gtatatcatg
     4921 aaagcatcgc cggcgttcac ccaccaactc ttcacacgga tttcgtatac atagctaggg
     4981 cctcctgatt ttggcgtcgc cgtaatcctt tcccaattgc tgccgacatt tttatatgtc
     5041 actttaccgg tagcatcaac atcgacccag gaggcagagc ttgaaaactc ataatcagcc
     5101 gccgtttttc caggggcaaa gttgtcatta ttcaactgat aatacgcccc ggtgaatccc
     5161 tgcgaaggga atgtagttgt cggtaggtca gtaccattga ctgacactgt accgctcatt
     5221 attttgtctt ctgcgcgagt gaattgtatt gtggtactca gaccagcttg atgaacacca
     5281 ttcaatacag ggatcagcgt tgcgataccc tctccgccgc ctgtaacagt ggctttgtac
     5341 tcgcctgaga aatttttact gtagtcaatt gcactaactt gcacataggg cacgttggta
     5401 cctgactgca caaattccag cccttcagaa actttgatcg gatggcctga tatatcgtgc
     5461 agaacaagat gtagctcagc actatcggta aagtctcctt tcaatgatga ccgattgttc
     5521 ttaaggacgg actgcgaggt gtctgccggg ccagccacca gcgttatatc tactgtttgc
     5581 attccaccag cggtcgtgac tgcgcttacc gtgacgctac ctgtcatcgc tcctttcacg
     5641 cttgttgtcg cgattccgtt ttgatcggtc accgctgtta atgacgttaa tgtggcagag
     5701 ccgcttttta aggcgaagta cacagttaaa ccttcgatca ggttaccact gccatcctca
     5761 accgttgcct ttaaggtact taaatcactg ttggtggcgg cgatagtcga tggagcagcg
     5821 ataaagctag caacatgggc ggtgcttgag ttgccagtga ctttcacgat tgtctgtgtc
     5881 tgtattgtta caccgttatg gaacgatgca gtcaccgtat atgtaccgac tttattgctg
     5941 gtcagaacga ctggagcctg acctgaagag ttggttctca cttttccgcc acttaacgtc
     6001 gcaccttctg gcgttacgct aaagttgatg acctgaccct ccactggagt gccatttgca
     6061 gaagttagcg ttgccgtcag agttgcacct gttggggaat tgacaccgat aagcgggctg
     6121 gacgccgaga gtgtcagttt ttgatcgatt accaccagat ccttctcata agaggatcca
     6181 ttcgccaggg atgctttcac catatacgaa ccgtttcttt cgggcgtcat agtgacctcg
     6241 gctataccat gcgtatcagt agagacaata ttttggctga tggtcatgtg ctctggtggc
     6301 tctgcactga atgttacaga ttcattaaga atcggattgc caaactggtc gttgacgtga
     6361 gctttaactg ctacgtcttg tgcgaccatg acctgacctt caggtatctc cagactggta
     6421 atcgttgcag aattaatatc tgcttttgca ttcagtaatc gcgagatgac ttcagtaggt
     6481 ttatctgcca gagaggctga aactgttttc agtccgacct cggtgcttgt cacaagaatt
     6541 tcagcgaaac cccgatcatc cgtttcaacg ctggtgctgc ttagcgtgac ggaggttccg
     6601 cggaaactaa cttttatgcc ttctaccggg ttgccgttag cgtcagtgac gcttgcagtc
     6661 atggtcgcgc cctcggtcgt gctgacgacg aatgagtata cagaggttaa ggaggctagt
     6721 tttgcggtac cagcatcggc aatcaaagtc acctgtttcg tatcactgac accataattg
     6781 ttcacgctaa ctgtcacggg gtaggtgccg gattttgtac cgctcagtgt aacttcagcc
     6841 ttgccgttga tgtcagtaat ggcggaaccg ccttgtccga gagtaaagct tgcgctcaca
     6901 tctgccggta gcgtgaatgt caccgcctca ttggctaacg ggttgccgtt tccatcagtc
     6961 actgttgcct gcagcctggt catcccgacg ttattagcga taaagttgtc ctcggtaacg
     7021 ttaagattaa cctgcgctgt gagcgtatcc gcaataaaat tcaccaccaa ctgctcactc
     7081 ttaccgccag ccatcgatgc tgtaacagta tgagcgcctg cttttgtacc tttcagcgtg
     7141 actttcgctt tgccttcagt atcagtaacc gctttaccgc catcgctcag cgtgaagttc
     7201 gccttcacat cttccggcag agtaaatgtt acttcagtgt tggctatcgc attgccctct
     7261 gtatcagcga ctgttgctgt tagtgtcgtg agatcgttat tgtcggcaat caccggatcc
     7321 ttcgaggctg ccagcgtgat ttcggcattc gcgacgttcg gcacataggt caccgtttgt
     7381 tgtatcgaat cgccattttc gagggttgcc gtcacttgat aaaccccggc ttttgtagcg
     7441 gtaaagcttg cgtaaaaatt gccgtcgtga ttggtcgtat atatagcgtt attactggga
     7501 gtcacgcctt cacttggtga aacgctgagg gtacctcctg ctggggtaca ccgttgccgt
     7561 aattatcctt cacctcaata tacagattgg tagtgtcgcc tgcggagact gtatcaagaa
     7621 gtgcctgtaa caaggtgaga tgtgctgtag acgcatcagc gttgacatta attgatgtgc
     7681 taagcgtgga gctaccattt tccaggctgg cctcaacggt atccggtctc gctcctgatt
     7741 ctatcgagga gcgggtattg gtataagtga cggtagcctt accctgttcg ttcgttacgg
     7801 cttggccgcc attcgtcatt tcggctgtcg ctgcgttgct ggtgaagttc acagtcacac
     7861 ctttcaccgg aaagccatta ttatcaatga ctgtagcggt gattacgcta ccgatgctgt
     7921 tctgtggggt accggcgatt atgctgcctg ggtcaggcgt caactcgata atttttgccg
     7981 ccgttgtgtc gccaataaag tgcacagttt tgttgtcgct ggcaccagta ttagccagtg
     8041 atgcagtaac agtctgctca ccaaaggcaa cgcctgcgag agtggcctgc gcgatgccag
     8101 actcattggt attactttcc cctggggtca gggtgaggcc tgaagaagct gtgctgaatg
     8161 ttaccggaag attgttcacc tcattgtcga actgatcttt gaccgttgca gttagcgttg
     8221 cgctatcgac gccattacct gtgatctcat tttttgatat ctgaagaaca accagagccg
     8281 aggttttgtc cgccacaaac gttaccggct gtgaatcact ggtattgtta ttactcagcg
     8341 ttgcggtaac cgtatgcgta cccgcttttt tacctttgag cgtgacatgc gcttccccat
     8401 tggccctggg tgatggcaat accgttattt tcgagggtaa agtttgccgc cacgtcctgt
     8461 ggcatggtga aattcaccgt tattcccgcc accggatgat tcgacggatc tttcaccgtc
     8521 gccgtcagct gcacgctgtc gctgttattc gtcaccacct gttgagcagg gatgttcagc
     8581 gtaacctgcg cgttgctggc atcgggcgca atattgacta tcttcgtatc gttgttgccg
     8641 ttaggcagtg tggcttcggc tgtatgaaca cccaaaaccg tgcccttaag ggtaacttca
     8701 gcaataccgt tctcattagt attacgcgcg ttcagactca gttgcgtgtc tgcgggggag
     8761 gtgcggaaga ctactgacag atttttaacc acgttatcaa aaggatcttt cactgttgct
     8821 gtcagggtcg tctcatccac gccattccca atgatttccg ctttcgatgt ttgtagaacg
     8881 acaaccgctc tgtctttatc cgccacaaac gtcattggct gtgcatcgct gacattgctg
     8941 ttagccagac gagccgtgat catatgcgtg cccgctaacg tgccggtcag ggaggcgatt
     9001 gcaaccccgt tactatccgt catgcctttt ccgccgttgc taatcgagaa ccgacttgcg
     9061 acgtcgtttg gcacagagaa ggtgatttct ttatctttta gtgggttgcc atttttatcc
     9121 tgcaaggttg cagtcatatg ttgcggagct gtgttggtga cagtgatatc acctgaaggc
     9181 acactgaggg tcagggctgc agtactttga tcaccgataa aattcacctg ttgattagcc
     9241 tgagaaccag agctcacaga ggccgtaacc gtataatcac catttttcaa actggtcagc
     9301 gtagctgtgg cgatcccgtc gtggctattc acttcggttt ggctcagttt cgctgctgct
     9361 gaattaacat tgaaggtgac cttgacgtca ttgagcaggt tgccttttgc atcccgaact
     9421 gtcgcggtca ttgtggcgct gtcattgccg tcagcgacca cttcattttt cgacttctgc
     9481 agatcaacct gtgcggtact cgagtcgccg acaaaactga cgattaacgt ttgtttcacg
     9541 ccattttcaa gggtgacttc aaccgtgttg tcttcctgct tactactttt cagatcaata
     9601 gtcgccagac cattaacatc cgtttttgcg gtgttttgat tgttaaagga agttgccgat
     9661 ccgcttaata ccgcaaacgt gacggtatga tcattgattg ggttgcttcc ttcatcagcg
     9721 acattgaccg agacggtgtt tgctgcattc tcattggcga gcacaccatt attgctggca
     9781 gataatgtcg caattttcgc tgactgcggg ttggcgtcga tgataaatcc agcggtatgc
     9841 aaatcttcat tccagttttg cattaatagc ttcgcagtaa gcccactgcc tttggtatag
     9901 gcggtatagg ttgccttata gacgccatct gcggtttctt tccagtctgt agtgacacca
     9961 ggtttcacat tgtcgatgct gactgcggta ttcagttgct gtttttgctc cttaacaggt
    10021 ttgtcatttt catctctcag ttctaccgtt acctcgatag gattgccgga gagataacgg
    10081 tccttatcaa tcttaattga cgagtgagtc cgggatgacg aaacagaaat gatattcacc
    10141 acggcggggg ctttagccgc atccacaccg ttcagctgtg gcataagcgt cagcgtgcca
    10201 gacgtcgcac ctgtggtcag gatctgggta tagcttccgt caccattatc tttccagtca
    10261 gaaagggtga tgtcctgaac accttcgtga cgcgtcgaga gcaccagccc gataacagga
    10321 ttacctgctg catcatgcgc aataaaagtc agtgtggcgg ttgaatggga atccgcgctc
    10381 aacgtctggg tacttaacga taccgaggaa tctttctggc ttaacgtagg tgcctgaacg
    10441 actaccatgc tctgttcacg attcgaacaa attgcctttg acatcttcag cggtgacttc
    10501 aatcggccag gtgttatcgg tttctggcgt actggtgaac cggtacgccg gcagggtaac
    10561 cagaatatct ttacccgttg taaccacttt accaccggca gcttccagag cggtggcttc
    10621 gacgttatag cctttcaggg catatttggt ttgtagcaac gaaaccagtg atttcacttc
    10681 tcctgacttc cctgtcacgg ggtctgtcag ggacaggcga accagttctt ttttgcgata
    10741 ttccagaacg atgttgttgt tgcgatccac cagatcataa cggctgcttg caaggctacg
    10801 ccgtgcagcg acttcattcg ggtcaagctg tttctgcatt gcgctgccag gttgccaggt
    10861 aaaatcgacg gcaaaacggg tgtcattttc gccctgttta ccctgacgtt gctacgcgct
    10921 gaaggtcatc agcgggaagg gggtatagtt aagtccagcg gttatggcat gaggattact
    10981 ttgccgatcg tctttatcga acagggccac ttcatcgcca taatactgtt catagaccag
    11041 tttaccgcca aggtgcggcc aggcgggtag ccagccttct gcgcgtacat cccagccatt
    11101 ggccgggcgt gcttcataat cgttgtccag ttcaggtgcg ctgcgccagt tggtcagtcg
    11161 caaatagccg ttactgctta attttagata gtcgcgccag tactccgcgc caatgccggc
    11221 gcgggagtgg taacggctaa gatcgtggtc gaaaaagaag ttgatgcccg acatccatgt
    11281 gggagtgaaa tgacgccaac ctaagccgtt gttaatctgc gtacgctcgt cagtacgatg
    11341 gagagtatgc tgactgaaaa agagattatc aggcgtttca taccacggat ggagaaaatc
    11401 gaactgggag ttcttcaggc taaaatcttc atccacgccc agcgtgattc ttgcggtacc
    11461 gaagcggctt aaccagtctg tcattgcgcc tgaagcctga gaagaggcca tccacgcgcc
    11521 atatttgccg cttgctcgct gttcatatcc tcggcgagca gagacccgat ttgctgtgaa
    11581 gtactggcta tctgttgctc aaggttgcca ctgctattac ccggtggcgg ggttaaatta
    11641 ttttcactaa cttgtgccgg gacatccagt tcatcaccct ggcggacatt atcaaaacct
    11701 cgagcaaact tacgaaactg gttgagtttg cgtaactcag ccaccgaaat accgaaacgt
    11761 tcggcaacgc tttgggccga ttccagcgct ccaagggtgt agggcaccgt attggcattt
    11821 gcaatggcaa tttgtgcagg aactggttgt tgggttgcgg cgtttaccac accttgtgct
    11881 gccgcagcca tagggaacac aagttgagtt accaggcaga tacccgcagt taagcggcgt
    11941 agtttgatac ccatcccgca taagatttgt cggtcattta tttcttctcc acttctcttc
    12001 ttcgtagcca tgcttacctt ccctgaacga gctaacataa atgcgccctt tctagcacag
    12061 cgaaaaaatg taaatcctga tagcaatatg attgaaaatt aataactttt cgttaggcag
    12121 ttttgggcgt gagttgcaag aggggagact actgaattac tcaagtttta taatcgaggg
    12181 gaaaatggtg atgacgccat agcaaaacgc cctcaaccat aaaggtcgag ggcgcttaag
    12241 atgttaaaaa cccgctatcc gttaaaaaac aatgttcaac taaggttagt gacattgcac
    12301 taagaagcga atttttagaa tttggctcct ctgactggga tcgcctttgc cagtaactag
    12361 ctaataatta agctaatcct gaatttcctt tttgtcaaga ccaccagaat gaccaccaat
    12421 cgaagctggt gatcattttt gtacatttgg ttatcgctat ttaatttatg aaacttgggt
    12481 tgttgtacgg cctgaaccta cctcgtttgg attctaagat ctgcaggtga tttttatctg
    12541 cagatcctat atcagtaagt tttttattct atgcttagat cttctgcata aattaaaact
    12601 ttttcaggag agcctatttg cacatagttc aggcattcag caaacaagaa gtgattatta
    12661 agttcatca
//
LOCUS       DAJOWB010000128        12556 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJOWB010000128 DAJOWB010000000
VERSION     DAJOWB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.128"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            43..852
                     /locus_tag="OHO85_003926"
     CDS             43..852
                     /locus_tag="OHO85_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HCQ2673235.1"
                     /translation="MKKAIIFLLSFYSCVGFAEPQQARSDYSIEESQAKVNKILHTTS
                     LYRNGLSYNERVAEISSRFLGTPYQPHTLIGSSSMQERLVTNPSTVDCFTFLDYVRSM
                     AHASSWQTYVSELVKTRYTNGMIDFTGRKHFFTDWAVISPRNAQDVTQDISPYTITVN
                     KQLNQKNKKQEYVKGLGIISRRISYIPASAIDKEVINKLQTGDYVGIYSTKRGLDVSH
                     VGIIIKDHNNIWFRNASSLAKNRKVVDSPFIRYMATKPGIVVLRDKTDQYP"
     gene            complement(869..1798)
                     /locus_tag="OHO85_003927"
     CDS             complement(869..1798)
                     /locus_tag="OHO85_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase"
                     /protein_id="HCQ2673236.1"
                     /translation="MILASSFLLRKRLFCFIKEVAVKLFINDLTVMDFSFLDAESGLI
                     GDSLIVDIILEGDLNAESMVMDFSHAKKSIKHEIDKLADHVLIVPEQNSHIIVSHAGT
                     TTEVAMLRKNGETQCFISGPQESFWLVQTDNINSRCLESQIEKHLLACLPQGVKDITI
                     TLRPESINGDSYHYSHGLKKHRGNCQRIAHGHRSAIRIFVDGERSHMWEQKWATRWNN
                     AYLLSREDVVTVTTLSPRGVAYWHKGLTCSSWRSSQGYFEIMLCSEVVDILPCDTTVE
                     SLALFIRQSIEHGLPAAKIEVHAFEGVGKGAIA"
     gene            complement(1865..2425)
                     /locus_tag="OHO85_003928"
     CDS             complement(1865..2425)
                     /locus_tag="OHO85_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008455674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCQ2673237.1"
                     /translation="MLRKNPSGHTPVVSTKAYIDPTAVICGRVIIHDYVYVGPYAVIR
                     ADELNADGDMDPIIIHSHSNIQDGVVIHSKSGAPVTIGSGTSIAHRAIVHGPCQVDER
                     VFIGFNSVLFNCHIQTGCVIRYNAVVDGVTLPENTYIPSTERVGPDSDLKSYSRVDPA
                     SLQFSEEVASTNVKLVEGYQKLRNEF"
     gene            complement(2415..3350)
                     /locus_tag="OHO85_003929"
     CDS             complement(2415..3350)
                     /locus_tag="OHO85_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016531967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HCQ2673238.1"
                     /translation="MSIAAKQTLCPGMLPDIQSTKGDGEGESLSWVGMEQIDLPIDIA
                     GRPVSAKVNAGINLLSSPEAEKGIHMSRLYLLLDELTQGEITPALLQHVLKAFLVSHQ
                     GRSDEASIEISGDLLLSRKSLNSNHSGWKAYPLTLSAELRQSFTVTLKVGIPYSSTCP
                     ASAALSRHVAGLQFSKDFGNRIDRLPAAEIADWLVEKGMPATPHSQRSWAWVSIRLNP
                     EAKSLPVIELIDYAEVALGTAVQTVVKRSDEQAFAVANGQNLMFCEDAARRLNNVFRC
                     APFCEAFDIRVEHQESLHPHNAVARIHWKGSKNVT"
     gene            complement(3414..3836)
                     /gene="dksA"
                     /locus_tag="OHO85_003930"
     CDS             complement(3414..3836)
                     /gene="dksA"
                     /locus_tag="OHO85_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004150576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCQ2673239.1"
                     /translation="MTKPTRAEREKILAMLPEDYMNPVQQDYFRRLIRHERQEVLDHI
                     DQMKQQLQDNPETGDEGDIAIREEQLRLLFRQIDRESRLLPKYDAALMRLEKGEYGFC
                     RDTGEPIGLQRLLLRPTAELSIEAKTKEEKVEIQYRKK"
     gene            complement(3907..5106)
                     /locus_tag="OHO85_003931"
     CDS             complement(3907..5106)
                     /locus_tag="OHO85_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCQ2673240.1"
                     /translation="MKRTPLIILNGFLGAGKTTLLKNLLTQAHKRRMTVSVIVNDMSE
                     LDVDGVLIANTEIVDAASNNFVSISADSISSRSGIQKLDSALKNLLEKRSADFILLET
                     SGSSHPLPLVRYLREHTQVSLKAFLSLVDTVMLNDDYDGGKKLIPVFQEHLNKGTRGV
                     ESLLAEQIMFCNKLLLTKNDRLPFYVVTEVARAIHPLNPQVAIMAVPWGNLQLDELLS
                     MPDYDFHRVALLIDELQDAIDAVLPDETDKKYDISWRVIEDDRPFHPQRLWDTCHRFM
                     GAGVYRSKGFFWLPGRDDLALLWNQAAGSINLEFISYWKAGVLTHTDNSLTKEERAAI
                     RQQLAKMPGRFGDRRCRLTVIGESGEIDDFALALRQCLLTEEEILWWQQGGIFHDPWP
                     TKVARLA"
     gene            5204..5656
                     /locus_tag="OHO85_003932"
     CDS             5204..5656
                     /locus_tag="OHO85_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCQ2673241.1"
                     /translation="MTTPVAKTYDWKQTLRTHGLRATPSTLATLQCLEHASQAMSHDD
                     LTQQLGEQAPDRVTLYRILERLMQVSIVQRYTDSARTQRFALTQRAAVGLFECDNCHH
                     VIPIEQDPALEAAIELVKSHLTGHGMTEREITLSSHGICPDCNRTITK"
     gene            5738..7120
                     /locus_tag="OHO85_003933"
     CDS             5738..7120
                     /locus_tag="OHO85_003933"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCQ2673242.1"
                     /translation="MIKLTNTLGMQKELLVPVKESKIGMYVCGVTVYDLCHLGHARTF
                     VAFDMIVRYLRFRGYDVTFVRNITDIDDKIIQRARDTGEDWRSLTERMVHEMHIDFAK
                     LGILPPNIEPRPTEHIPQIISMIERLLHRQFAYIAENGDVMFSTASAKGYGKLSGQDI
                     TMLRAGTRIEVNEYKRDPLDFVLWKRAKPGEPVWPSPWGEGRPGWHIECSAMNCHYLG
                     THFDIHGGGSDLIFPHHENEIAQSTSANDGPYVNYWLHTGMVTVNKEKMSKSLNNFFT
                     LRDILNRFDAQSVRFFLLSAHYRKPLNYSEENIHLARASLTRLYTACQFADGSRSEQH
                     DAWRARFCEAMDDDFNTPQALAILFELTREINRLCGTNDDEAAALATTLKDLGNVLGL
                     LEQSADAFLKNRSDLHPAELQLIEKLIHQRSEARRMGKWDKADRLRNELAEQGIDLED
                     VGENTSWRIR"
     gene            7191..8321
                     /locus_tag="OHO85_003934"
     CDS             7191..8321
                     /locus_tag="OHO85_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HCQ2673243.1"
                     /translation="MNQPREVVIVGAGPAGVGMAALLRRSAIQDILVVDSHEVGASFM
                     RWPEETRFITPSFFSNPFGQPDLNSVTPDSSFALFCGEEHPGGKTYASYLKAVLDEYQ
                     IPVMAPARIAKVALLSSGNFILITEAGEKLETRSLIWATGEFQFPDRQIFPGADICCH
                     YGDVTSWKDFRKGEYIVIGGYESAVDAAVNLLENGSSVKMLTRSAPWSTNHISDPSLS
                     LSPYTRERLNRVMNHRLFEIYEDADVCEVIRMPGPGSSYKVHTTNGRAWATDEVPVLA
                     TGFQCGGGARQLAAFFEWNDDGYPVLTDEDCSTLFPGLYLVGPHVRHAGNIYCFIYKF
                     RQRFPVVAESITRHLGLSSEGVRDWWVLPSEPDCCADEDCAC"
     gene            8333..9982
                     /locus_tag="OHO85_003935"
     CDS             8333..9982
                     /locus_tag="OHO85_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014907277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HCQ2673244.1"
                     /translation="MQSFVLMGLESAGKSTLFNILTESAASDERNFRGSTVVCREGLI
                     NDAGICLVDTPGIRFQSDSETTKLALDALNQHDGILVVLRATHAQQEWQTLCHLIPPQ
                     AKHVVILLTFADKIRKGLLEVTEYLSETSGAPVLAVNAREAGSNVRQGIVQLLLQDKP
                     APSAVSLPRQKIPVINLLAEFPQQTIFEHRWCGKVAAIVCLFLLFAVPVWGAWLLSDF
                     IQPVIDSAVIQPLKNITTSWPDVLKTLFVGNYGLFSLGLYSFVWAFPVVVLIGLSLSL
                     TDDSGLKERITATLDPWLRKLGLSGQDLIPVLSGFGCNVVAVFQSRSCSRCTRHACIS
                     MISFGSACSYQTGATLSLFNAAHQPWLFIPYLSLLFFTGAIHTRLWNGSLKPGQNQRL
                     TELTWLQWPRWRNVTWMLKNILRQFITQAMPLFLIICIVAGMLDYAGITRWLSETTAP
                     LLHLFKLPAELMPGIIFSLLRKDGLMVLNQDGGSLIQSLSTSQLLLLVWLASTLMACL
                     VTVFTIAREINWRFAAAVAGKQVLSSLVVALVISQLFIHEA"
     gene            complement(10249..11472)
                     /locus_tag="OHO85_003936"
     CDS             complement(10249..11472)
                     /locus_tag="OHO85_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCQ2673245.1"
                     /translation="MSEVSANFHGDKRLPVTVLSGFLGAGKTTLLNHILNNREGRRVA
                     VIVNDMSEVNIDAALVREGGAELSRTDEKLVEMSNGCICCTLREDLLLEVNRLAKEGR
                     FDQLVIESTGISEPLPVAETFTFAGDDGESLSTVARLDTMVTVVDAYNFLKDYGSEDS
                     IQSRGESLGEGDERSVVDLLIDQIEFCDVLVLNKVDLISEAQKEKLMAILRSLNPRAR
                     IVVSQFGQVPLGNILNTGLFDFEQAAQAPGWLKELRGEHTPETEEYGITSFVFRARRP
                     FHPTRFWQVMENELDGVVRSKGYFWLASRPEFAGSWSQAGGIARQGLGGMWWASVPKG
                     RWPEDAESLKFIMSNWIDGIGDARQELVFIGMDMNESELRNRLDSALLTDAEMAEGPQ
                     KWRHYSDPVEPWFEE"
     gene            complement(11509..11910)
                     /gene="hisI"
                     /locus_tag="OHO85_003937"
                     /pseudo
     CDS             complement(11509..11910)
                     /gene="hisI"
                     /locus_tag="OHO85_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212825.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
     gene            12061..12543
                     /locus_tag="OHO85_003938"
     CDS             12061..12543
                     /locus_tag="OHO85_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004150583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCQ2673246.1"
                     /translation="MSNSPYSLSTRDTALSSTDCSGRRELIESKKIRITPLRERVYNF
                     VSHAKEKGISAYQILELMKKYNPNAKPATVYRSLDYLQQAGIIVKIECCSKFIKKNNL
                     SSDVVTIFLICSNCGTIIQHTDHLIHTYIEKKAIDYGCAVQKKDIEVKVICPDCRGKG
                     "
BASE COUNT         3290 a         3067 c         2868 g         3331 t
ORIGIN      
        1 tttaaatata atgagaaata tctctgagga agttgatatt ttatgaaaaa agcaattatc
       61 tttctccttt cattttatag ctgcgtggga ttcgctgagc ctcagcaggc acgaagcgac
      121 tattctattg aagaaagtca ggcgaaagtt aacaaaatcc ttcataccac ttcgttgtac
      181 agaaatggcc tcagctataa tgaacgcgtt gctgagatat cctcccgttt cctcggaacg
      241 ccttaccagc cgcatacact gattggatca tcttcgatgc aagagaggct ggtcactaat
      301 cccagtaccg tggattgttt tacttttctt gattatgttc gctccatggc gcacgccagt
      361 agctggcaaa cttatgtatc tgaacttgtt aagactcgtt atacaaacgg aatgattgat
      421 tttactggca gaaaacattt tttcacggac tgggcggtca tatctccgcg aaatgcacaa
      481 gatgttactc aggacatcag tccgtatact attactgtca acaaacagct aaaccaaaag
      541 aataaaaaac aagaatatgt taagggtctc ggcattatct cacgacggat aagttacata
      601 ccggcttctg caatagataa agaggtaata aataaactgc aaacaggtga ttatgtgggt
      661 atttattcaa caaaaagagg cctggatgtt tcacatgttg ggatcattat caaagaccac
      721 aataacatct ggttcagaaa tgcatcgtca ttagcgaaaa atagaaaggt agttgactca
      781 ccttttatta gatacatggc tacaaaaccg ggtattgtag ttttacgaga caaaacagat
      841 caatacccct gaaaagtaga atagtgtgtc aggcaatagc gcctttcccc actccctcaa
      901 aggcatggac ttctattttc gctgcgggta aaccgtgctc aattgactgc ctgataaata
      961 aagccagtga ctcaaccgtg gtatcacacg gcagaatatc tactacctca gaacaaagca
     1021 tgatttcaaa gtatccctga gaactccgcc aggaagaaca tgtcagcccc ttatgccagt
     1081 atgcaacgcc acgcggtgaa agtgttgtca ctgtaacgac atcttcccgg cttaatagat
     1141 aagcgttatt ccatcgcgtt gcccacttct gttcccacat atggcttcgc tcaccatcca
     1201 caaaaattct gatcgcagag cgatgcccat gcgctattct ctggcaattt ccgcggtgtt
     1261 ttttcagtcc atgcgaatag tgataactgt ccccattaat actttccggc cgtaacgtta
     1321 tcgttatgtc ttttactcct tgcggcaaac acgccagtaa atgtttttct atttgggatt
     1381 caaggcaacg ggaattaata ttatctgttt ggaccagcca gaaactctcc tgcggcccgg
     1441 aaataaaaca ctgggtctcc ccattcttac gcagcatggc gacttccgtc gtagtgcctg
     1501 catgactaac aataatatga ctattttgtt ccggcacaat caggacatga tcggccagtt
     1561 tatcaatttc atgtttgata ctttttttag catgtgagaa atccattacc attgactcag
     1621 cattcagatc tccctcaaga ataatatcaa caataaggct atcgccaatc aacccggatt
     1681 ccgcatcaag gaaactaaaa tccatgaccg tcagatcatt aataaaaagt ttcaccgcta
     1741 cctctttaat aaaacagaaa agcctcttcc gcaaaaggaa agaagaggca agtatcattt
     1801 tgctacatta ataacaaatc gttaataata cggtgttacg ttaacggcac tagccgagcc
     1861 atactcagaa ttcattgcgt aacttctgat aaccttcgac cagtttaacg ttggtactgg
     1921 ccacttcttc tgaaaattgt aaagaagcgg ggtctaccct cgaataactt ttaagatcgg
     1981 aatcaggccc cacgcgttcc gttgatggaa tataggtatt ctcaggcaag gtaacgccat
     2041 ccacaactgc attatagcga ataacacaac cagtctgaat atgacagtta aacaacacgc
     2101 tgttgaatcc aataaatacc cgctcgtcaa cctgacaggg gccatgcact attgcacggt
     2161 gggcaataga cgttccgctt cctattgtca ctggcgcgcc gctttttgag tgaataacca
     2221 ccccgtcctg aatattagag tgggaatgga taatgatagg atccatatcc ccgtctgcat
     2281 ttagctcgtc agcccggata accgcataag gtccgacgta aacataatca tgaataatga
     2341 cacgtccaca aattaccgcg gtgggatcga tataggcctt tgttgacacc actggggtat
     2401 gacctgatgg gtttttacgt aacattttta cttcctttcc agtgaatacg ggcaacggca
     2461 ttatgaggat gcaggctttc ctgatgctca accctgatat caaatgcttc acaaaagggt
     2521 gcacaacgaa aaacgttatt gagccgacgg gcagcatctt cgcaaaacat cagattctga
     2581 ccgttagcca ccgcaaaagc ctgttcgtcg ctccgcttga caaccgtttg cacggctgtc
     2641 ccgagagcaa cttcagcata atcaatcagt tcaatcacag gcagtgattt tgcttcagga
     2701 ttaagacgga tactcaccca tgcccagctt cgctggctgt gcggggttgc cggcattcct
     2761 ttctcaacaa gccagtcggc tatttctgct gccgggagcc tgtcgatcct gttaccaaag
     2821 tctttgctaa actgcaaacc cgcgacgtgg cggctaagtg cagcggaagc cgggcaggtg
     2881 gacgaataag ggatacccac tttgagggta accgtaaaag attgcctgag ttcggcgctc
     2941 agagtcagag gataagcttt ccagccggag tgattagaat ttaacgactt acgcgacagt
     3001 aacaaatccc ctgaaatctc aatactggct tcatcactac gtccctgatg agataccaga
     3061 aatgctttca acacatgttg caataatgcc ggtgtgattt ccccttgtgt cagttcgtcc
     3121 agaagaagat aaagacgaga catatggatc cccttttcag cttcaggtga actgagaagg
     3181 tttatgccag cattgacctt tgctgatacc ggcctgcctg cgatgtctat cggcaggtca
     3241 atttgttcca tgccaaccca ggataatgac tcgccctcac cgtcaccctt agtggactga
     3301 atatccggta gcattccggg gcataaggtt tgtttggctg caatactcat atatacctgc
     3361 ctgatctgat atttactctc atccctaccc tgccccctgc ctgagccacc gctttatttc
     3421 tttcggtact gaatctcaac tttttcctcc tttgttttgg cttcaatact taattctgcg
     3481 gtgggcctga gtagcaggcg ttgcagaccg ataggttcgc ctgtatcccg gcagaagcca
     3541 tattctcctt tttcaagccg cataagcgct gcatcatatt tcggcaacag gcggctttct
     3601 ctgtctatct gtctgaacag cagacgtaac tgctcttcac gaatggcgat gtcaccttcg
     3661 tcacccgttt ctggattatc ctgcagttgt tgcttcatct ggtctatatg atcaagcacc
     3721 tcctgccttt cgtggcggat aagccgccgg aagtagtcct gttgcacagg attcatgtaa
     3781 tcttctggca gcatcgccag gattttttct cgttcagcgc gggtaggttt agtcatgtcc
     3841 tgtaccgata aaattgcgta atgaaacgtt ataacataac attaactgcc gctgcaaaat
     3901 aacctgttaa gccagccggg ccactttcgt gggccaggga tcgtggaaaa tccccccctg
     3961 ctgccaccag agaatttcct cttcagtgag taaacactgg cgcaatgcga gggcaaaatc
     4021 gtcaatttcg ccgctctcgc caataacagt caggcggcag cgccggtcac caaatctgcc
     4081 aggcattttt gccagttgct gacggatagc tgcgcgttct tcctttgtca atgaattatc
     4141 agtgtgagtc aggaccccgg ctttccagta gcttatgaat tcgagattga tgctccctgc
     4201 tgcctggttc cacaataatg ccaggtcatc ccttcccggt agccagaaaa accccttgct
     4261 gcggtaaacg cctgcgccca tgaagcgatg gcaggtatcc cacagccgct gcggatgaaa
     4321 tggacggtca tcctcaatga ccctccacga aatatcgtat tttttatcgg tttcatccgg
     4381 caaaactgcg tcaatggcat cctgaagctc atcgataagt aacgccaccc gatgaaaatc
     4441 gtaatccggc atggaaagca attcatccag ctgtaaattt ccccagggca cggccataat
     4501 tgcaacctgg ggattaaggg gatgtatggc tctggctacc tcagtaacca catagaaggg
     4561 taaccggtcg tttttggtta ataagagctt attacagaac ataatctgtt ctgccagcag
     4621 gctttccact ccccgggttc ctttattgag gtgctcctgg aaaaccggaa taagcttctt
     4681 tccaccgtcg tagtcatcat taagcatgac cgtatcaaca agagacaaaa atgctttgag
     4741 acttacctgg gtatgttctc gcagatagcg gaccagggga agaggatggc tgctacctga
     4801 ggtttccagt aaaataaaat ccgcagaccg tttttcgagc aggtttttca gcgcggaatc
     4861 cagcttctgg atcccgcttc tgctgctgat actgtctgct gaaatactca caaaattgtt
     4921 actggctgca tcaacgattt ctgtgttggc tatcaggacg ccatccacat caagttcact
     4981 catgtcatta acgatgacgg atacagtcat tcgccgtttg tgtgcctgtg taagaaggtt
     5041 ttttagcaga gtagttttcc ctgcccccag aaaaccatta aggataatga gcggtgttct
     5101 tttcatagca tccagcctta tgccgctaaa cgttaatgca atcaagttgc aataataccg
     5161 gcttcctgcc aaaattcaaa tgacatgact cggaggggga aatatgacaa caccagtagc
     5221 caaaacgtat gactggaagc aaacgttgcg cacacatggt ttgcgggcaa cgccatccac
     5281 ccttgctaca ctgcagtgcc ttgaacatgc cagccaggcc atgagccatg acgatttaac
     5341 tcagcaactg ggcgagcagg ctcccgatcg tgtgaccctt taccgaatac tggaacggct
     5401 aatgcaggtc agtattgtcc agcgttatac cgactcggca agaactcagc gtttcgctct
     5461 gacccaacgg gctgccgtag gactttttga atgcgataac tgccatcatg ttattcctat
     5521 tgagcaggat ccagccctgg aagcggccat tgaattggta aaatcacatc tcactggaca
     5581 tggtatgacc gaacgggaaa ttaccctttc cagtcatggt atctgtccgg actgtaatcg
     5641 tactatcacc aaataatttt tttcttattc cggacgactg ttaattagcg ccaggcgtgt
     5701 tcgtgtgctg ctatctaaaa ataatgaggt aaacgtaatg attaaactaa caaacacgct
     5761 tggtatgcaa aaggagctat tagttccggt taaagagagc aaaatcggca tgtatgtatg
     5821 cggcgtgacg gtttatgacc tttgccatct tgggcacgcc agaacctttg tagcttttga
     5881 tatgattgtc cgctatctgc gttttcgcgg atacgatgtg actttcgtgc gtaacattac
     5941 tgatatagat gataaaatta ttcagcgcgc ccgtgatacg ggagaggact ggcgcagcct
     6001 gacagagcgg atggtgcatg aaatgcacat tgattttgca aagttaggca tcctgccgcc
     6061 aaacatcgaa cctcgtccca cagagcacat cccacaaatc atcagtatga tcgaacgact
     6121 tctgcatcga caatttgctt acattgcaga gaatggtgac gtcatgttct ccactgcatc
     6181 ggcaaaaggc tatggaaagc tttcgggaca ggatattacc atgcttcgtg ccgggacccg
     6241 catcgaagtg aatgagtata aacgcgatcc gctggatttt gttctctgga agcgggcaaa
     6301 acccggcgag cccgtctggc catcaccctg gggtgaaggg agacctggct ggcatattga
     6361 atgttccgca atgaactgcc attaccttgg tacccatttc gacattcatg gcggtggttc
     6421 tgacctgata tttccccatc atgaaaatga gattgcccaa tccacctccg ccaatgatgg
     6481 cccttacgta aattactggc ttcacaccgg catggtgacg gtgaataaag agaagatgtc
     6541 gaaatcgctg aataactttt ttaccctgcg cgatattctg aatcggttcg atgcccagag
     6601 tgtgcgcttc ttcctgctaa gcgcacatta tcgtaagccg ctgaattata gcgaggagaa
     6661 tattcacctc gcgcgcgcct cgctgacacg gctctacacc gcatgccagt tcgctgacgg
     6721 gtctcgatct gaacagcacg atgcatggcg ggccagattc tgtgaggcaa tggatgatga
     6781 tttcaataca ccccaggcgc ttgccatttt atttgaactg acaagagaga ttaacaggct
     6841 ttgcggaacg aatgatgatg aagccgcagc acttgccacc acgctgaaag acctggggaa
     6901 tgtgctgggt ttgcttgaac aatcagcgga tgcctttttg aaaaaccgca gtgatcttca
     6961 tcctgcagaa ctgcaactta ttgaaaagtt aattcaccaa cgcagtgagg cacgacgtat
     7021 gggcaaatgg gacaaggccg atcgtttaag aaatgaatta gcggagcaag gcattgatct
     7081 tgaagatgtc ggcgaaaata cctcatggcg tatccgataa gttgtccctc tggatgtaaa
     7141 aaatttcaat atcaattgtt atgttataac gtaatgcaaa gggtaatttt atgaaccaac
     7201 cacgcgaagt cgttattgtc ggtgcgggtc cggccggggt gggtatggcg gcgcttcttc
     7261 gtcgtagtgc catacaggat atcctggtcg ttgacagtca cgaagtaggt gcctccttta
     7321 tgcgctggcc ggaagaaacc cgttttatta ctccttcttt tttttccaat ccgtttggac
     7381 aaccagacct gaactctgtg acccctgaca gttcatttgc gcttttctgc ggtgaagaac
     7441 atcctggggg gaaaacttat gccagctacc ttaaggccgt actggatgag tatcagatac
     7501 ccgtcatggc tcccgccagg attgcaaagg ttgcgttgct cagttccggt aattttatac
     7561 tgataacaga agcaggagag aagctggaaa cacgctcttt aatatgggct acaggcgaat
     7621 tccagttccc agaccggcag atttttcctg gagcagatat ttgttgccat tacggtgatg
     7681 tgaccagctg gaaggatttc aggaaaggtg aatacattgt tataggcggc tatgaaagcg
     7741 cggtggatgc cgctgtaaac ctgctggaaa atggctcatc ggtaaaaatg ttaacccgct
     7801 cagccccctg gtcaactaac cacatcagcg atcccagtct atcgctttcc ccttataccc
     7861 gtgagcgttt gaacagagtg atgaatcacc gtcttttcga aatttatgaa gatgcggatg
     7921 tctgtgaagt aatcagaatg cccggcccgg gaagttcgta caaagtccat accacaaacg
     7981 gacgcgcctg ggctactgac gaggtgcctg tgcttgctac cggcttccag tgtggcggag
     8041 gagcaaggca actggctgct ttttttgaat ggaatgatga tggctatccg gtcttaacgg
     8101 acgaagactg ctcaaccctt tttcctggtt tgtaccttgt cggccctcat gtgcggcatg
     8161 ccggtaatat ttattgcttt atctataagt tccgtcagcg ctttcccgtc gtggcagaaa
     8221 gtatcacgcg tcatctgggc ctgtcctctg aaggagtacg ggactggtgg gttctgcctt
     8281 cagagcctga ctgctgtgcc gatgaggact gtgcgtgctg aggtccgtgt ttatgcaatc
     8341 attcgtttta atgggattag aaagtgcagg aaaatctacc ctgtttaata tcctgacgga
     8401 aagtgctgcc agtgatgagc gtaatttcag gggatcgacc gttgtatgtc gggaaggtct
     8461 cattaatgat gcagggatct gcctggttga tacgccaggc atacgttttc agagcgacag
     8521 tgaaaccaca aaactcgccc ttgatgcgct aaatcagcat gatggcattc tggtggttct
     8581 gcgggccacg cacgctcagc aggagtggca aacgctctgt cacctcatac cgccacaggc
     8641 aaaacacgtt gttatactcc tgacctttgc tgataagatc agaaagggcc ttctggaagt
     8701 gactgaatac ctgagtgaaa catcaggtgc gccggtactg gcagtcaatg cccgggaagc
     8761 tggcagcaat gttcgccagg gaatagtgca gttgctgttg caggataaac ctgcaccctc
     8821 cgctgtctct ttaccccgtc aaaagatacc ggtaattaat ctgctggctg aatttcctca
     8881 acaaacgata tttgaacatc gttggtgtgg aaaagttgct gcaatagtat gccttttcct
     8941 gctgtttgcc gttccggtat ggggggcatg gctgctgtca gattttattc agcctgtcat
     9001 tgacagtgcg gttattcagc cgcttaagaa tataacgacg agctggcccg acgttctgaa
     9061 gacactattt gtcggcaatt acggtctttt tagtctggga ctttattcgt ttgtatgggc
     9121 atttcctgtg gtggtactga tagggttgtc tctttcgctg actgacgaca gtggacttaa
     9181 ggaaagaatt acagcgacgc tggatccctg gttgagaaaa ctcggcctca gcggtcagga
     9241 ccttattcct gtattaagcg gtttcggctg taatgtcgtg gcggtttttc agagccgttc
     9301 gtgttcgcga tgcacccggc atgcttgtat aagtatgatt agctttgggt ccgcttgcag
     9361 ctatcagacg ggggcgacgc tttctctttt taatgccgcc catcaaccat ggctctttat
     9421 cccctatctc agtctgctgt ttttcacagg cgcaatacat acgcgcctct ggaacgggtc
     9481 gttgaagcct ggccaaaacc agcgtctgac agagctcacc tggctacaat ggccacgctg
     9541 gcgaaatgtg acatggatgc tgaaaaatat cctccgtcag ttcattacgc aagccatgcc
     9601 gttattcctg attatctgca ttgttgcggg gatgcttgat tacgcaggga tcactcgctg
     9661 gctgagtgaa acgacggcac ctttacttca tctttttaaa ctgccagcag aactgatgcc
     9721 cgggatcatt ttttcactgc taaggaaaga tggtttgatg gtgctcaatc aggatggcgg
     9781 cagcttaata cagtctctca gcacatctca gttactgcta cttgtctggc tcgcctctac
     9841 gttgatggcc tgccttgtca cggtatttac catcgcccgg gaaataaact ggcggtttgc
     9901 agcagcagtg gcgggaaaac aggtattaag ttctctggtg gtggctctgg tgatatccca
     9961 gttatttatt catgaggcat aagtatgatc cccaagactt accagcaatg gtaccggtgc
    10021 atcacagaag aatgtggaat tcccttgacg caggcattta ttgcatcccg tctggcagtc
    10081 cttgagtccc aagatcatca ggaaaccaga cgttttgtct cgctatatgg gcaacaacat
    10141 ctgaatagtg ttgtacagtg gttcaaaaaa gctggggaag aattgttgta aatctgcccc
    10201 cttttcgctt tcatacgaag gcacccgaca aatgcgggtg ccttcgattc attcttcgaa
    10261 ccagggctca acaggatcgg aataatgccg ccacttttga ggcccttctg ccatttctgc
    10321 atccgttaac agggcggaat ccagtctgtt gcgcagttct gattcattca tatccattcc
    10381 aataaaaacg agttcctgac gggcatcacc aatgccatct atccagttag acatgatgaa
    10441 cttcagtgac tcagcatcct ctggccaacg tcctttcggc acactagccc accacatgcc
    10501 ccccagcccc tggcgcgcaa tgccacctgc ctgcgaccat gaacctgcga attccggacg
    10561 gctggccagc cagaagtaac cttttgatct gacgactcca tccagctcat tttccatcac
    10621 ctgccagaaa cgggtcggat gaaaaggccg gcgggcgcga aagacaaagc tggtgatccc
    10681 atattcttct gtctccggcg tatgttcgcc ccggagttct ttaagccagc cgggtgcctg
    10741 agcggcctgc tcgaaatcaa acagcccggt attaagtatg ttgcccagcg gaacctgtcc
    10801 aaactgtgaa acaacgattc tggcccgtgg attaagcgaa cgtaaaatcg ccatcagttt
    10861 ttctttttgc gcttcactga taaggtcaac tttattaagg accagcacat cacagaattc
    10921 aatctgatcg atcagcaagt caacaacgct cctttcatca ccttcaccta acgattctcc
    10981 gcgtgactga atgctgtctt ccgaaccgta gtctttcaga aaattgtacg catccaccac
    11041 agtgaccatg gtgtcaaggc gggccaccgt cgaaaggctt tcaccgtcat cgcccgcaaa
    11101 ggtaaatgtt tcagcaacag gcagaggctc agatataccg gtggactcaa tgacaagctg
    11161 atcaaaacgc ccttcttttg ccagacgatt aacttcgaga agaagatctt cgcgcaatgt
    11221 gcagcaaatg caaccgttgc tcatttctac cagtttttca tcagtccggg agagttctgc
    11281 accaccttcc ctcacaaggg cagcatcaat attcacctca gacatatcat taacaatgac
    11341 agcaacacga cggccttccc ggttgttcag aatatggtta agcagtgtcg ttttccctgc
    11401 tcccaaaaat cccgaaagta cggtaaccgg caggcgttta tcgccatgaa aatttgcact
    11461 gacttcggac attaccttac atctcctcag atattcgttg tgttcggatt aaccaggatc
    11521 gttgagcacg gttagctcat cgcctttaat ggcgttgtaa aaacaactcc gccgacctgt
    11581 gtggcaggct ccaccttttt gatccacaag cagtagtacc gcatctccgt cacaatccag
    11641 acggatatca tgtacttgct gaacgcagcc ggaggtctca ccttttcgtc aaaggcatga
    11701 tcgggatcgc gaccagtaac aggctctgcc agtcaataag gtttcatcaa gagcctgttg
    11761 attcatccag gcaagcatca acacctctcc agtgtcatat tgctgtgcaa tcgcagcgat
    11821 caatccatga gaatcccagg gtattgatgc caggatatct tcaagtttaa aaactgcacc
    11881 tgcatgggct ttttccagta agagaaacat tggcacctca cttatcatgt gttatgttat
    11941 aacgtaacaa taaaatttgc cagttcttct ttccttacat ggcacgatca aaacatcagt
    12001 agatttccat cgtttcagga caagaaaaga taacatcgct tgaaaattac aggaggcccg
    12061 gtgagtaata gcccatattc tctaagtaca agggacactg cattatcgtc tacggattgt
    12121 tccgggcgga gagagctgat tgagagtaag aaaatacgaa tcacaccgct ccgggagaga
    12181 gtttataatt tcgtcagtca tgctaaagaa aaaggcatta gcgcatacca gatactggaa
    12241 ttaatgaaga aatataatcc gaatgcaaaa ccggctacgg tttatcgttc tctggattat
    12301 ttgcaacagg cagggattat cgtaaaaata gaatgctgta gcaaatttat caaaaaaaat
    12361 aatttatcct ctgatgttgt gactattttt ttgatttgct caaactgcgg aacaataatc
    12421 caacatactg accacctcat tcatacttac attgagaaaa aagcaataga ttatggttgt
    12481 gctgtacaaa aaaaagatat tgaagttaaa gtaatttgcc ctgattgtcg aggaaagggt
    12541 taaatcacca atacga
//
LOCUS       DAJOWB010000129        11984 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJOWB010000129 DAJOWB010000000
VERSION     DAJOWB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.129"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            266..1036
                     /locus_tag="OHO85_003939"
     CDS             266..1036
                     /locus_tag="OHO85_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCQ2673247.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNQDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1027..1500
                     /locus_tag="OHO85_003940"
     CDS             1027..1500
                     /locus_tag="OHO85_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCQ2673248.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSESLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1608..2147
                     /gene="dnaT"
                     /locus_tag="OHO85_003941"
     CDS             1608..2147
                     /gene="dnaT"
                     /locus_tag="OHO85_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCQ2673249.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALDEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELTSFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2150..2887
                     /gene="dnaC"
                     /locus_tag="OHO85_003942"
     CDS             2150..2887
                     /gene="dnaC"
                     /locus_tag="OHO85_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCQ2673250.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2936..3430
                     /locus_tag="OHO85_003943"
     CDS             2936..3430
                     /locus_tag="OHO85_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCQ2673251.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3684..5975
                     /gene="opgB"
                     /locus_tag="OHO85_003944"
     CDS             3684..5975
                     /gene="opgB"
                     /locus_tag="OHO85_003944"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCQ2673252.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDHCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTMWKGKTAFKDTVIDMARYKSNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6114..7136)
                     /gene="lgoD"
                     /locus_tag="OHO85_003945"
     CDS             complement(6114..7136)
                     /gene="lgoD"
                     /locus_tag="OHO85_003945"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCQ2673253.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7275..8189
                     /locus_tag="OHO85_003946"
     CDS             7275..8189
                     /locus_tag="OHO85_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCQ2673254.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8404..9765
                     /locus_tag="OHO85_003947"
     CDS             8404..9765
                     /locus_tag="OHO85_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2673255.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9814..11478)
                     /gene="tsr"
                     /locus_tag="OHO85_003948"
     CDS             complement(9814..11478)
                     /gene="tsr"
                     /locus_tag="OHO85_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCQ2673256.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWAYYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11597..>11984)
                     /gene="hpaR"
                     /locus_tag="OHO85_003949"
     CDS             complement(11597..>11984)
                     /gene="hpaR"
                     /locus_tag="OHO85_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCQ2673257.1"
                     /translation="RPIVKRHNLTEQQWRIVRILAESPSMDFHDLAYRACILRPSLTG
                     ILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRAQTQIEEAYRQIEAQFTAEKM
                     QQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         2912 a         3167 c         2919 g         2986 t
ORIGIN      
        1 ttgttaacat taaaaaacgc acctggttat ctattatcct gattcgtaaa ggccaaacaa
       61 taaattcatt atgatgataa tattcatgtg atagtttgtt tgcggcgaga gataattcgc
      121 tttttatcac cgagcttctc ccgataacca ggattaatca gcgcaatttt gatcttaacc
      181 taccgcaaaa caaccgtgct tcatgataca aatggattct cattatctga acaatacgca
      241 gcacgtctat gacaaagggc gagttatgca aactgagcaa cagcgagccg taacacggct
      301 ttgtatccag tgtggattat ttcttctgca acatggtgcg gaaagcgcgt tggttgatga
      361 gctttcctca cgactgggtc gggcgctggg aatggacagc gtcgaaagtt ctatctcttc
      421 gaacgccata gtgctgacaa ccattaaaga tgggcaatgc ctgacatcga cacgaaaaaa
      481 tcaagatcgc ggcattaata tgcatgtggt gactgaagtc cagcacattg tgattctggc
      541 ggagcaccat ctgctggatt acaaaggcgt agagaaacga tttagccaaa ttcagccatt
      601 acgttaccca agatggctgg tagccttaat ggttggcctt tcctgcgcct gtttctgtaa
      661 actcaataaa ggtggctggg atggtgccgt catcaccttc tttgccagta cggccgcgat
      721 gtatatccgc cagctactgg cacaacgtca tcttcatcca cagatcaact tttgcctcac
      781 cgctttcgcc gccaccacta tttccggttt gcttttgcaa cttcccacct tcagcaatac
      841 acccaccatt gcgatggccg ccagcgttct gctgctggtg ccgggatttc cgttgataaa
      901 tgccgtcgcc gatatgttta aaggccacat caataccgga ctggcacgct gggcgatcgc
      961 cagtctgctg acactggcta cctgcgtcgg cgtggtgatg gcactgacga tttgggggct
     1021 acgcggatgg gtgtgatcga gtttctgttt gcattggcgc aggatatgat cctcgccgcc
     1081 attccagcgg tcggctttgc gatggtgttc aacgttcccg ttcgggcatt acgctggtgt
     1141 gcgctgcttg gcgcgattgg tcatggttca cgaatgatct taatgaccag cgggctgaat
     1201 attgagtggt caacctttat ggcttctatg ctggtcggca ccattggtat tcaatggtcg
     1261 cgctggtatc tggcgcatcc gaaagtgttt accgtggcgg ctgttatccc catgttccct
     1321 ggcatatcgg cttataccgc aatgatttcg gcggtaaaaa tcagccagtt aggctacagc
     1381 gagtcattga tgattaccct gttaaccaac tttcttacag cttcatcgat tgttggtgca
     1441 ttatccatcg gtctttccat tcctggatta tggttgtacc gcaagcgccc tcgcgtataa
     1501 aattgcctcg cttaaagcac acggatgaga gacagcctcc tctcctccgt gtgttactat
     1561 aaaagttatc tcccttctcg ttcatcgttc catatttgag aaacagtatg tcttccagag
     1621 ttttgacccc ggacgtcgtt ggtattgacg ccctggtaca cgatcaccaa accgttctgg
     1681 caaaagctga aggcggtgtg gttgccgtat ttgctaacaa tgccccggcg ttttatgccg
     1741 tcacgcctgc acgcctggct gaactgctgg cgctggacga aaagctggcg cgtccgggaa
     1801 gcgatgtcgc tctggacgat caactctatc aggaaccgca agctgctccc gttgctgtac
     1861 ccatggggaa attcgccatg tatccggact ggcaacccga tgccgacttt atccgcctgg
     1921 cggcgctatg gggcgtggca ctaagagagc cggtgaccac cgaagaactg acctcattca
     1981 ttgcctactg gcaggcggaa ggtaaagtct ttcaccatgt acagtggcaa caaaaactgg
     2041 cgcgcagcct gcaaatcggt cgtgccagca acggcggact gccgaaacga gatgtgaata
     2101 ctgtcagtga acctgacagc caaattccac caggattcag agggtaacga tgaaaaacgt
     2161 tggcgacctg atgcaacgcc tgcaaaaaat gatgcctgcc catatcaaac ctgcattcaa
     2221 aacgggtgaa gaactactgg cctggcagaa agaacaagga gcgatccgct ccgccgctct
     2281 cgaacgtgaa aatcgggcga tgaaaatgca gcgcaccttt aaccgctccg gtattcgtcc
     2341 actgcatcag aactgctcct ttgagaacta tcgcgttgag tgtgaagggc agatgaatgc
     2401 gttaagcaaa gcgcgccagt acgtcgaaga gtttgacggc aacatcgcca gcttcatctt
     2461 ttctggtaag ccaggaaccg gcaaaaacca tctggcggcg gcaatctgca acgagctgct
     2521 gctacgcggt aaatccgtat tgatcatcac cgtggccgat attatgtcgg cgatgaaaga
     2581 taccttcagg aatagcggta ccagcgaaga acaactgctt aacgatctga gcaacgttga
     2641 tctgctggtg atcgatgaga tcggcgtgca gaccgaatcg aaatacgaaa aagtgatcat
     2701 caaccagatc gtcgatcgcc gctcttcttc caaacgccca accgggatgc tgaccaacag
     2761 taatatggaa gagatgacca agttgctggg cgaacgcgtg atggaccgta tgcgcctggg
     2821 taacagtttg tgggtgatct tcaactggga tagctaccgt agccgggtaa caggtaaaga
     2881 gtattaatcc ccgtttctgg cggtatactg cgacacaatt ccaccttgag taatgatgat
     2941 gaaaactgtt aaacatcttc tgtgctgtgc cattgccgcc agcgcattaa tttccaccgg
     3001 ggtgcatgct gcgtcctgga aagatgcgct ctccagcgcc gccagcgaac ttggcaacca
     3061 aaacagcacg acacaggaag gcggttggtc gctcgcgtca ttaactaact tgcttagcag
     3121 cggaaaccag gccttaagcg cagataacat gaacaatgcc gcaggcattc tgcaatactg
     3181 cgcgaagcaa aagctggcgt cggtaaccga tgccgaaaac atcaagaatc aggtgctgga
     3241 aaagctgggc ctgaacagtg aagagcaaaa agaagacacc aactatctgg acggcattca
     3301 gggtttgctg aaaaccaaag atggtcagca actcaatctg gataacatcg gaacgactcc
     3361 gctggcagaa aaggtgaaaa ccaaagcctg cgatctggtg ttaaagcagg ggctgaactt
     3421 catttcctga accgcgaaac gccgcgtttt agctgttacc cggctgacgc ggctctctcc
     3481 tgcccttttc cctgctcctg gacggttttc cccctgttac acgggaagcg accgcgatca
     3541 aaaaagtctg tttataaact aattcctaac aacatcgcat ttttttgaca ctagaattgc
     3601 agcaatatgg cgacgccatt acattgtgct tctcaaaaag cttaaacgtt ggctaaacgg
     3661 cccggtttag tgaggatcgc ccgttgtcag aactactctc tttcgccctt tttctcgcct
     3721 ctgtgctgat ttacgcatgg aaagcgggac gtaacacctg gtggtttgca gccacattaa
     3781 cggtgctggg gctatttgtc gttttaaata tcaccctgtt tgccagcgac tattttactg
     3841 gcgatggtat taacgacgcg gttctctata ccttaaccaa cagcctgacc ggtgctggcg
     3901 tcagcaaata catcctgccg ggtatcggca ttgtgctggg gctgacagcg gtgttcggtg
     3961 cgctgggctg gatcctgcgc cgtcgtcgcc atcatccgca ccattttggt tacagcctgc
     4021 tggcgctctt actggcgctg ggttcggtgg acgccagccc ggcatttcgt cagataacgg
     4081 aactggtgaa atcccagtca cgcgatggcg acccggactt tgcggcttat tataaagagc
     4141 cgtcgaaaac tatccctgac ccgaaactca acctggttta tatctacggc gaaagtctcg
     4201 agcggaccta ttttgataac gaagctttcc cggatctcac gcctgaactg ggcgcgttga
     4261 aaaatgaagg cctggatttc agccacacgc agcagctgcc aggaacggat tacacgattg
     4321 cgggcatggt ggcttctcag tgcggcatac cgctgtttgc cccctttgaa ggcaacgcct
     4381 ccgcctctgt ctccagcttc ttcccgcaga acatctgtct gggcgatatc ctgaaaaact
     4441 cgggttatca gaactatttc gtgcagggcg cgaatctgcg ttttgccggt aaagatgtgt
     4501 tcctgaagtc gcatggcttc gaccacttat acggctcaga agagctgaaa agcgtggtgg
     4561 ccgacccgca ctatcgcaac gactggggat tctacgacga taccgttctc gatgaagcgt
     4621 ggaaaaagtt tgaagagctt tcccgctcag gtcagcgatt ctcactgttt accctgacag
     4681 tcgataccca tcacccggat ggttttatct cgcgtacctg taaccgcaaa aaatatgatt
     4741 ttgacggtaa gccgaaccag tcattcagcg cggtaagttg cagccaggag aatatcgcgg
     4801 cgtttatcaa caaaatcaaa gcgtcaccgt ggtttaaaga taccgtcatc gtcgtctctt
     4861 ctgaccattt agcgatgaac aacacggcgt ggaaatacct taataagcag gatcgcaata
     4921 acctgttttt tgtcattcgt ggcgacaagc cgcagcaaga gacgctggca gtgaagcgta
     4981 acacgatgga taacggcgcg acggtgctgg acattctcgg tggcgataac tatctcggac
     5041 ttggtcgtag cagtttatcc gggcagtcga tgtcggaaat cttcctcaat atcaaagaga
     5101 aaacattggc gtggaagccg gatatcatcc gcctgtggaa attccctaaa gagatgaaag
     5161 agttcactat cgaccagcag aaaaacatga ttgccttctc gggtagccat ttccgtttgc
     5221 cgctgctgtt gcgggtttca gacaagcgcg tagaaccgct gccggaaagt gaatactcag
     5281 caccgctgcg tttccagctg gccgatttcg ctccacgcga caatttcgtc tgggttgacc
     5341 attgctacaa gatggcacaa ctttgggctc cggaactggc actctccacc gactggtgtg
     5401 tctcgcaggg gcagcttggc ggtcagcaaa ttgtccagca tgttgataaa acaatgtgga
     5461 agggcaaaac ggcatttaaa gatacggtca tcgacatggc gcgttacaaa agcaatgtcg
     5521 atacgctgaa aattgttgat aacgatattc gctacaaagc cgacagtttc atcttcaacg
     5581 tcgccggtgc gccagaagag gtgaaacagt ttagcgggat ttcccgtccg gagtcgtggg
     5641 gccgctggtc caacgctcag ctgggcgatg aagtaaaaat cgagtacaag catccgctgc
     5701 cgaagaaatt tgacctggtg attaccgcca aagcatacgg caataacgcc agccgtccta
     5761 ttccggtacg cgtaggcaat gaagaacaaa ctcttgtgct gggcaatgaa gtaaccacca
     5821 ccacactgca tttcgataac ccaaccgatg ccgacacact ggtaattgtg ccgccggaac
     5881 ctgtctcaac caacgaaggg aatatcctcg gacactcgcc gcgtaagctc gggatcggca
     5941 tggtggaaat taaagttgtg gaacgtgaag ggtaattcct gactgatgtg taggtcgcat
     6001 aaggtgcgac ctacacacct tctccggcct acaaaacatc attgagaaac agaaaaaaac
     6061 agcgcccaac acttccccgg acgctgcatg gcaactttaa taatctcgcc ttatcagaaa
     6121 gtaattacac ccttaatcaa ctcacgattg ttaatcacat cgcgctcgta ggtttctgcc
     6181 agcgtggcga acggatagcg atgggttaac atcatgtcgg cagtgatttt cccttctgcc
     6241 atcagtcgac cgactttagc gaaatcttcc ggcgtggcgt tgcggctgcc catcatcgtc
     6301 gtttcttttt tatggaattc cggatcggaa aactgcaact cacctttaaa caggccgaca
     6361 aataccaccg tgccgccgtg acgaatcaga ttcacggtgt tattcatcgc atgttgatta
     6421 cctgtcgcgt cgatcacttt ctgcgccagc gaaccaccaa actgcgcccg tagctgcgcg
     6481 tcaaaatctt cggctgacgg gtccagtaca ggtaattcca gacgcgttgc cacatgttca
     6541 cggcgcgctg gactggtatc cgccaccacc acctgtgcgc catcggcttt agcgattgcc
     6601 gccgcgccca gaccgattgg ccccgccccg accaccagca cctgctcgcc gggagcaatg
     6661 gctgcgcgac gcaccgcatg agcgctaata gcgaaaggtt caatcaatgc tgccgcctgc
     6721 gggtcaatac cgtctgcggg caaaatgttc gccaccggca cgctcaaata ctcactaaaa
     6781 ccgccatcct gatgcacgcc aatgactgaa attttttcac agcaattggt acgcccgctt
     6841 ttacacgccg ggcattgctg acaggcaaca taagggatca cagcaacttg ctgaccattt
     6901 ttaagatcag caatattttt acccagccca acaatctccc cacatatttc atggcctaaa
     6961 acacgtggat aactaaaaaa tggttgattt ccaccccagg catgaatatc ggtaccgcaa
     7021 atcccgacag acttaatttt tattaatgct tcattgtcac ccggaatagg tatctcgcgt
     7081 tgtttccaga ctaattcttt cggctgctgg caaattaaaa cattcatcgt agacatgatt
     7141 cggctctcca ttttttgttg ccttagatat caatgaaaat gaaaaatggc agcggaacgg
     7201 aaaatctgtt ttgtgaaaac acacgcataa aaatgtttta aatcgggttt taatggactc
     7261 acaaggagga aaatatgagt cgttcacaaa atttacgcca caatgtgatt aaccaggtca
     7321 ttgatgatat ggcgcggggt catatccctt ccccacttcc atcgcaaagt gcgctggcgg
     7381 aaatgtacaa catcagccgc accacagtgc gtcatattct cagccattta cgcgaatgcg
     7441 gcgtcttgac gcaggttgga aacgactatg ttattgcgcg taaacctgac cacgatgacg
     7501 gtttcgcctg tactaccgct tcaatgagtg aacaaaacaa agtgtttgag caggcttttt
     7561 tcacgatgat aaaccagcgc cagttacgcc caggggaaac tttttctgaa ctgcaactgg
     7621 ctcgggccgc gggtgtcagc ccggtggttg taagagaata tcttttaaaa ttcggccgtt
     7681 ataatcttat tcaaagcgaa aagcgcggcc agtggagcat gaagcaattc gatcaatcct
     7741 acgccgagca gctgtttgag ctacgcgaaa tgctcgaaac acattcactt cagcactttc
     7801 tcaacctgcc tgatcatgat ccccgctggc tgcaggcgaa aaccatgctc gaacgtcatc
     7861 ggctactgcg tgacaacatc ggcaatagct ttcgcatgtt ctcgcaactt gacagagatt
     7921 ttcactcctt actgctttca gctgccgaca atatcttttt tgatcaatcg cttgagatca
     7981 tctcagtcat ctttcatttt cactatcaat gggacgaaag cgatcttaaa cagcgcaata
     8041 tcattgccgt cgatgagcat atgaccattc tcagcgctct gatttgccgc agtgaccttg
     8101 atgccaccct tgcgctccgt aaccatttga attcagctaa acaatcaatg attcgatcca
     8161 ttaatgagaa cacgcgttat gctcattaag tacggattaa aaacagcaaa ctattagcgg
     8221 gtgcacggtt ttatcaaaag ataaataccg ggaaattccc gcttacctat gctcacaatc
     8281 agtcgactaa aaatgttttt gtgagcggta gtaaagtcct aaaactttaa cctgccgtta
     8341 atgtcaacct tgcgtaatgc acaattacgt aaacaattaa aattataaat acaggagtcc
     8401 ggcgtggaaa aagaaaatat caccctcgat ccgcgttctt catttactcc atcttcgtcg
     8461 gcagatattc ccgtgccacc agatggatta gttcaacgca gtacccgaat taaacgcatt
     8521 caaaccaccg ccatgttgtt attatttttt gcggcggtaa tcaattatct cgaccgcagt
     8581 tcgctgtcgg tagcaaattt aacgattcgt gaagaattgg gattaagtgc caccgaaatc
     8641 ggcgctttgc tctccgtgtt ttcactcgct tacgggattg cgcaacttcc ttgcggccca
     8701 ctattggatc gtaaaggccc acgcctgatg ctgggactgg ggatgttctt ctggtcactg
     8761 ttccaggcaa tgtctggcat ggtgcacaac tttacgcagt tcgtgttggt gcgtatcggt
     8821 atggggattg gtgaagcgcc gatgaaccca tgcggtgtaa aagtcattaa cgactggttc
     8881 aacatcaaag agcgcggacg cccgatgggc ttcttcaacg cagcttctac cattggcgtt
     8941 gccgtaagcc caccgattct ggcggcgatg atgctggtga tgggctggcg cgggatgttt
     9001 attaccattg gtgtactggg gatttttctc gccatcggct ggtatatgct ctatcgcaac
     9061 cgcgagcacg tagaactgac tgccgttgaa caagcttatc tcaatgcagg tagcgtcaat
     9121 gcccgccgag atccgctcag ttttgccgaa tggcgcagcc tgttccgtaa ccgtacaatg
     9181 tggggaatga tgctcggatt cagtggcatc aactacactg cgtggctgta tctggcctgg
     9241 cttcctggtt acctgcaaac agcctataac ctggatttaa aaagcacagg gttgatggcg
     9301 gctatccctt tcctgtttgg ggctgccggg atgctggtca acggttacgt tactgactgg
     9361 ctggtcaaag ggggaatggc tccgattaaa agccgtaaga tctgcattat tgccgggatg
     9421 ttctgttctg ccgcctttac gctggtcgta ccgcaagcga caacatccat gacagcggtt
     9481 ctgctgattg gcatggcact gttctgtatt cactttgccg gaacatcctg ctggggcttg
     9541 atccacgtcg cagttgcttc tcgcatgact gcgtcggtgg gcagtatcca gaactttgcc
     9601 agcttcatct gcgcctcttt tgcgccgatc attactggtt ttattgttga taccacccac
     9661 tcattccgtc tggcactaat catctgcggt tgcgtcaccg cagcgggggc actggcgtac
     9721 atcttcctgg ttcgtcagcc gatcaacgac ccacgtaaag attaatctcc ctatgcccga
     9781 taacattttg cttatcgggc atttttatgg cgattaaaac gtttcccagt tctccccgct
     9841 atctgccact gccattttac gcggcgtagc tggcgtcacg gtttttacca cagccgatgt
     9901 ttcacgctgc tgttgctgct gttgctgaat ccggaacact gccacagctt cggtcaggcg
     9961 actggcctgc tcttccagcg cggcggcggc ggcggcagat tcttccacca gcgcggcgtt
    10021 ctgttgagtt acccggtcca tctcagcaac cgctaagcca acctgatcga taccacggct
    10081 ctgctcatca gaagcagaag ctatttcgcc cataatgtcc gtcacgcggg tcacagcact
    10141 gacaatctcc gccattgttt ccccggcgct ttcgaccagc gtagagccaa catccacttt
    10201 ccccaccgag tcttcaatca ggcttttaat ttcacgagcc gcctgggcgc tacgctgggc
    10261 cagattacgc acttctcccg caaccaccgc aaaaccgcgc ccttgctcac ccgcacgcgc
    10321 agcctcaacc gccgcgttca aagccagaat attggtctgg aaggcaatgc cgtcaattac
    10381 gctgataata tcggcgattt tctgcgaact ggtggagata tcgcgcatag tctgcaccac
    10441 gttatccacc actttgccgc cgcgttgcgc cgtttcagaa gcacttaacg ccagatggct
    10501 ggcctggcgc gcattttcgg cgttctgctt caccgttgcg gtcagttgct ccatgctggc
    10561 tgccgtctct tccagcgaag cggcctgttg ctcggtgcgc gaagagagat cgttattgcc
    10621 ggtggcgatt tcgctggcac cgctatagat ggcattggcc ccgttgcgca catcaccgac
    10681 ggtacgcatc agctctccct gcatatggcg caaactctct gccagttgcc ccatctcatt
    10741 agagccatcc acctcaatcg gtttcaccag atcgccgcct gcaatatgac gaatgctgtc
    10801 aatcaggcga ttcattggcg ctaccagcga ggctttaata ccgaaccaga cggcgaagat
    10861 gaccgccagt acgacgatca tcacgcccac cagaatccac atagcctggc tgtaggaggc
    10921 attgttatcg ctgacggcga tatcatagag ccgatcgttt tgctccatgt aagccacata
    10981 ctgcttctcg aaaccgtcct gatatccctg ggtcggctga tcaaagaact cgttgatttt
    11041 gcctgcacct aatagttgga tcagctccgc cagcgcattg tgataaatat cgtaattacg
    11101 tttgatctct gccgctgcgg cggtgctctg acgcgggtca cgcggcaacg cttcgtaata
    11161 cgcccagttt ttttccgcct gtttcagcga aatactggca ctctgcatca gctcagcaac
    11221 ggttgaaccg ctaccaatat tattctgatc catcatgtag cggatacccg cgcggttgag
    11281 ggtgttacgc gtctgcaaca acgcgaccca gctgccattc agcgtggatt gctgctggcg
    11341 aatggtttgt aaaacagtga aattttcttt gtcattcttt aaggcattaa agaacagacc
    11401 gcctgatgtc agttgtaaaa ggccaaaaac agccaaaacc agcagtaagc tggtcacgat
    11461 cttgatacgt tttaacatgg tttctctttc ctgtggaccg atgcaagatt ttcggcctgg
    11521 aaagcaaaaa ctttatgaaa aggtgtaacc cgccatccat ggaaaaacca ggcgctggtt
    11581 atcagatact aaaaagttat tcattatcgc ccgggatgtc ttcttgtcgg gaattgccca
    11641 gagcaataaa ttcctctaac agatgggtta actgttgcat tttctcggca gtaaactgag
    11701 cttcaatctg ccgataggct tcttcaatct gtgtctgagc gcggttatac agcgcctgcc
    11761 cctctttggt tagcgagata tagagcttcc gctgatcatt gatcggcttt aatcgcaaca
    11821 ctaaaccgtc gcgttccatc cgcgtaagga tcccggtcag gcttgggcgc aaaatgcagg
    11881 cgcgatacgc cagatcgtga aaatccattg atgggctttc cgccagaata cgcacgatgc
    11941 gccactgttg ctcagtcaaa ttatgccgtt taacgattgg gcga
//
LOCUS       DAJOWB010000130        11906 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJOWB010000130 DAJOWB010000000
VERSION     DAJOWB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.130"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(115..1758)
                     /gene="yeeO"
                     /locus_tag="OHO85_003950"
     CDS             complement(115..1758)
                     /gene="yeeO"
                     /locus_tag="OHO85_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCQ2673258.1"
                     /translation="MLRHILTAKNLLSNPIFNFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPAVSE"
     gene            1763..1838
                     /locus_tag="OHO85_003951"
     tRNA            1763..1838
                     /locus_tag="OHO85_003951"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1796..1798,aa:Asn,seq:gtt)
     gene            complement(1876..2826)
                     /gene="cbl"
                     /locus_tag="OHO85_003952"
     CDS             complement(1876..2826)
                     /gene="cbl"
                     /locus_tag="OHO85_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCQ2673259.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGIVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2928..3845)
                     /gene="nac"
                     /locus_tag="OHO85_003953"
     CDS             complement(2928..3845)
                     /gene="nac"
                     /locus_tag="OHO85_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCQ2673260.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGEFN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVMSSPVMEKRQWQLVS"
     gene            4172..4247
                     /locus_tag="OHO85_003954"
     tRNA            4172..4247
                     /locus_tag="OHO85_003954"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4205..4207,aa:Asn,seq:gtt)
     gene            complement(4303..5238)
                     /gene="ldtA"
                     /locus_tag="OHO85_003955"
     CDS             complement(4303..5238)
                     /gene="ldtA"
                     /locus_tag="OHO85_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCQ2673261.1"
                     /translation="MMRRVNIFCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGRYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5300..6379)
                     /gene="cobT"
                     /locus_tag="OHO85_003956"
     CDS             complement(5300..6379)
                     /gene="cobT"
                     /locus_tag="OHO85_003956"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCQ2673262.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6391..7134)
                     /gene="cobS"
                     /locus_tag="OHO85_003957"
     CDS             complement(6391..7134)
                     /gene="cobS"
                     /locus_tag="OHO85_003957"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCQ2673263.1"
                     /translation="MSKLFWAMLSFITRLPVLRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7131..7676)
                     /gene="cobU"
                     /locus_tag="OHO85_003958"
     CDS             complement(7131..7676)
                     /gene="cobU"
                     /locus_tag="OHO85_003958"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCQ2673264.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            8224..8628
                     /locus_tag="OHO85_003959"
     CDS             8224..8628
                     /locus_tag="OHO85_003959"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCQ2673265.1"
                     /translation="MKSLTAVRKKSPNYPVEFKIKMVELSHRPEISVAQLAREHGIND
                     NLLFKWRQYWREGKLRPPSTTENNVPELLPITLDAEDVVPTTSPRSQPVAAATPESLN
                     ISCEVTFRHGSLRLNGAISENILNLLIRELKR"
     gene            8625..8972
                     /gene="tnpB"
                     /locus_tag="OHO85_003960"
     CDS             8625..8972
                     /gene="tnpB"
                     /locus_tag="OHO85_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCQ2673266.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDDPMSGHVF
                     IFRGRNGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            9021..10559
                     /locus_tag="OHO85_003961"
     CDS             9021..10559
                     /locus_tag="OHO85_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc22 family transposase"
                     /protein_id="HCQ2673267.1"
                     /translation="MSSSLPDDINALKRLLAEQEALNRALQEKLNEREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLKALQKESDTLTGRVDDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAASCCPECGGSLSYLGEDAAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLISKYAEHTPLYRQSEMYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALQDYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNA
                     GSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKN
                     YLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVA
                     LPTE"
     gene            complement(11627..11906)
                     /locus_tag="OHO85_003962"
                     /pseudo
     CDS             complement(11627..11906)
                     /locus_tag="OHO85_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3175 a         3218 c         2723 g         2790 t
ORIGIN      
        1 tgaactacag aggaatcgtg agaacgaggc gaatattagc gatgcccaac cacaatgtca
       61 aagcctgttt tttaaatttg aaatcgtttg ctgaaataat ctgcattttg tcgtttattc
      121 cgacacagct ggcttttttt cacacttttg cggttcgggt cgagggtatt tccatagcca
      181 acgtccagta accattcgcc agtaaaacag cacggcccgc acagcccagt cggcaaacat
      241 tcccatccag acaccaacca caccccagcc aagcatgatg cccagcacat aaccgacgac
      301 aacccggcaa ccccacatgc tcaacataga aacccacata gcgtaacggg cgtcacgagc
      361 ccccttaaat ccagcgggca gcacccatga ggcggaccaa ataggcataa ataaagcatt
      421 tagccaaatc agaatcacaa cgacatgttt aacctgtgga tcctgggtgt aaaacgatgc
      481 cataaccccg gcaaagggag ccgttagcca ggcgatggcc gttaatccaa gagtggaaag
      541 ccagaacaca tgccgcaact gaatctctgc ttgcgctatc tgtcctacac ccaaccttcg
      601 gcctgtaatg atcgtagaag cagagccgag cgcacttccg ggtaagttga taagagccgc
      661 aattgaaaac gcgataaaat ttccggcaat aacactggtc cccatcccgg caacgaacat
      721 ttgggttaat aaccgaccac tggtaaataa cactgattcg acactcgcgg gaataccaat
      781 ccccatgact tcccagataa tgctaaaatt cagcggttta aaatagctct ttaacgaaat
      841 ccttagcgca ggattaaaac caatcgccaa cacccacaaa attgcaactg cgccaatata
      901 acgagaaatg gttaaaccca acccagcccc gacaaatccc agtcccggcc aggagaaaag
      961 gccgtaaatc aatatgccgc taataataat attaagaata ttcaggctac cgttaatcag
     1021 tagcggtatt ttcgtattcc ctgcaccacg aagtgccccg ctaccaatta gagtgatggc
     1081 agctgctgga taactgagta ccgtcagctc cagataagtc aacgccagtg ctttaacttc
     1141 tgtcgtggca tcacccgcga cgaaatcaat aatttgctcg ccaaaatgat gaataagcgt
     1201 tgccaacagt acggcaaaca acgtcatgat caccaatgac tgccgcgtcg ccaccctcgc
     1261 tcgtcgtcta tcccgcttac cgagactaaa tgccacaacg acagtagtac caagatcgat
     1321 agcagcaaaa aaagccataa tgaccatatt gaagctgtcc gccaatccca cgccggccat
     1381 cgcatctttt cccagccagc tgaccagaaa agtgctcaga acccccatca acaggacaca
     1441 ggcattctcc atgaagatag gaacagcaag cggggttatc tcgcgccaga acaacacttt
     1501 gtagctcttg cgtttagcgt gccagcgagt gccgtgaaca acctggcgta aagcagagga
     1561 gatattcaaa gccgacctta attgcagaaa gtgaaaccac atttcaaata atgagggaga
     1621 atcagcaaag ctgcaaagat tttcgccaac aaattgtctg caaatgcaac aaactgttga
     1681 tagaaacggc aaacagttgg gaaaattaaa aatcgggttt gacaaaagat ttttcgccgt
     1741 taagatgtgc ctcaacagcg attcctctgt agttcagtcg gtagaacggc ggactgttaa
     1801 tccgtatgtc actggttcga gtccagtcag aggagccaaa tttctgaaaa gcccgctttt
     1861 atagcgggct ttttgctata tctgataatc aatttcctct tcactgtttt ccatcacctg
     1921 ccgcttgata tcctcaactg aaagtcctgc attacaaagt tccagaaagc gccagacata
     1981 gttacgctga agttgtcctc gcttcagtcc caaccagaca gtattagcat caaaaagatg
     2041 ccgcgtatcc aggcggatta aattcttttc ctcttgttca ccgctggatt gttcggcaac
     2101 tattccgatc ccaagcccaa gagcaacata ggttttaatg acatcagaat cctgcgcact
     2161 taatacaata tctgccagca aacctttgcg ggcaaatgcg tcatcaatac gtgagcgccc
     2221 cgtaatcccc tgtcggtaag tgattaacgg ccacttcgct attgattcca gcgtcaacgg
     2281 tgtaatttgc gtcaagggat gatcgagtgg aacaagcaaa ctatggtgcc aacgaaacca
     2341 cgggaaggcg acgagctgcg ggtcattact caaacgctcg ctggcgatac caatatcagc
     2401 ttcgccattt tgcaacaacg tcgcaatttc ctgtggcgta ccctggatta gctccagccg
     2461 aacctccggg aaaagttcgc gaaaagcttt aatgacctca ggtaagctat aacgtgcctg
     2521 agtatgcgtc gttgcaatag tgagaacgcc agacgtatcg ttggtaaaca ggtctgcaag
     2581 ccgacgaaca ttactggctt cattcagaat acgttctgca atgaccagta atgctttgcc
     2641 cggttcagtc atgcccagca gtcgcttacc tcgtcgaaca aatatttcga tgccaagttc
     2701 atcctccagt tcccgaatat gacggctgac gccggactgt gaggtaaaaa gcatattcgc
     2761 aacctctgtc aggttgtaat cctgacgtgc agcctcgcgg attatcttta gttgttggaa
     2821 attcacggta aactccgggc agttcagatt ttccgttatt gttaaagtct aatgcccggc
     2881 ataacaaata ataaaaaccc gcatcttatt ccatcccgat ataacgctta gctcaccaat
     2941 tgccactgcc ttttttccat cactggagaa ctcatcactg acattaacaa ctctttcact
     3001 gcctgtgcct gtggtgataa gttcgctctg gcgggtaaat ttaatgacaa agagagactc
     3061 atggaaggtg tggtaatgcg tgacatccac ccgtttactg cgccacataa cgaacgcgcg
     3121 gccgattcgg gtaatactgc aacgcccatg ccgctggcaa tcgctgcggt aagcgtggca
     3181 atagactcaa tttcaccaat gacttttgcc gtgagtcgcc gtagggaaaa agcctcatca
     3241 acacgaagtc taacagcact gtaatcactg ggaagaaaga ggttcatttg cgcaatagca
     3301 ttaacatcaa cgctttgccc cggacaatct tgagtcccta ccaggaaaag atcttctttc
     3361 agtaaagcct gactggatac accagccaca ggggaatgct cataaatcac cgccatatcg
     3421 agttggtgat ttatcagttt ttcgttaagc actgcaccgc tattttcatg aagatagata
     3481 acgacctccg ggaattcagc gcgaaccgcc tgtaataagg gcatggtgat ggatgacgca
     3541 gcggttcctg gcgcaaagcc aatcgagact tgccccgata atgcctgacc aacgttatgc
     3601 accgccagtt gggcctgctc acactgacgt aaaatggccc gcgcatgggt atagagaatt
     3661 tttccggcgt cagttggcgt aacgccccgt tttgtgcgaa tcaaaagttg ttgattaaac
     3721 tcaccttcca gtgtggcaac ctgctggctg agcgctggtt gtgcgatatg caatacttca
     3781 gcagcctggg tcaggctacc aatatctaca atttttacga agtatttcag gcgtctgaag
     3841 ttcatgttgc ctccggtttt taagagtcgg cccaagtgcc gccattactt acaaccagaa
     3901 tgcaagatgc ttgccagttc tattttggcg ttgatgcgca agctaaccaa ctgtcaaata
     3961 agagattatg atagattcgt cattggctct ttttatctgc agtcgcattc acctgcccta
     4021 taaaaggagg gtatgcacca cgatggttca ttacccaata agattgaaag ctcaccactt
     4081 tgttgaaatt ggcagcaaac aaacaaaaaa atgcatttca ccctttgaca tcaccatgca
     4141 ctgccattaa tatgcgcccc gttcacacga ttcctctgta gttcagtcgg tagaacggcg
     4201 gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat tcaaaaaagc
     4261 ctgctttcga gcaggctttt tactttctaa ttatcaacgc tcttaaaaca tctgtcttga
     4321 accagaacta atttgcacag gcattcccga tcgacgttgc aacgcagcat ttgcgcgatt
     4381 tacatcaact tcttgcccgt tgataaacgc ccgcaaagat ggggttaccg gcaatggcac
     4441 ttttcggtca gactcatatt ctgcacgatt gcgcgacagc ggctcatgaa cttccagcca
     4501 ctttgaacca tccggttccg ttgtgtattt cactggctga tcaataattt gtacacgagt
     4561 gcctacagga acattatcaa acagatattt gatatcgtca ttacgcagac gaatacagcc
     4621 ctgacttaca cggagcccaa taccaaaatt ggcattggta ccgtggatgg catacaacct
     4681 gccaatataa atcgcgtacc gccccatggg gttatcgggc cccgcaggaa caaatgcggg
     4741 caaactctcc cctcgtttcg catattcgcg ccgggtgttc ggcgttggcg tccaggttgg
     4801 tgcttcttgt ttacgttcaa cggtagtcac ccagttacgc ggggtttctc gcccagcctg
     4861 gccgatacca ataggaaaga cttccactgt attactgtct ggcggatagt aataaagacg
     4921 catttcagcg acgttaacca caatcccttt acgaacagtg gcgggcaaaa tcagttgctg
     4981 cggaatggtg agttgtgagc caggcttcgg caaaaaaaca tcagcgcccg gattcgcttc
     5041 cagcatatta cttaaccctt gcccgtattg tgcggcaaaa gtctccagcg gctgagtatt
     5101 gtgatcagga acagttacag taagcgactg tcccactaaa cggctaccct ctggaggtaa
     5161 tggataagtt accgccaggc tattttggct ggcaaaaagc agagcaaatg agcaaaaaat
     5221 atttacacga cgcatcatgt ccctttccta agtcgcgaaa gctatccgtt aagtatagct
     5281 tttatcagac ttttcgtttt taactgttca aatcagaagt cgtattcccc ggtagaacaa
     5341 tattactggc agcaagttcg cccatgttgt tatatatcgc acaggcagct tcgatgatgg
     5401 gcatcgccag agctgcgcca ctcccctcac ctaaacgcat ctccatattg agataaggct
     5461 ccagccccaa atgcgacagc gcaatacgcg cccctttttc tgccgacagg tgagaaggaa
     5521 tgagatacgg tttgattgca ggagacatct ggcaggctgc gagcgcggca gcataagaaa
     5581 gaaatccatc cagcaacacg ggtaaaccgc aggaagcagc gcctaacatc acgccagcca
     5641 ttccgaccaa atcaaatcca cccacttttg ccaggacatc aacgccatcc tgcggatttg
     5701 gttgattcaa cgtaatcgcc cggcgcacaa catcaatttt attagccagt ttatctgtcg
     5761 gcaggtttgc gccaatccca accacttctt caggatcccg gccagtgatt gtgctgacta
     5821 ttgccgctgc cggtgtcgtg tttgccatcc ccagttcacc tacaccaaac agcgtgacac
     5881 cgttttttgc cagctcccgc gtataacata tgacgtccaa aagcaacttt tcagcctgac
     5941 ggcgactcat tgccggagct gaagcaatat tgccgctacc tcgtgcgaca cgcatgttga
     6001 taagcccggg gataggctca gcactatcaa taccaacatc aactacgtgg acgttagcgc
     6061 ccgcttgtgc tgccagcaca cacacgccgg ttgttccgcg ggtcatattt tcagcctgaa
     6121 tggctgtcac ttcttttggg gaaatagcga ccccttcctc ccagacgccg tgatcggcac
     6181 acataaccag taccgctttt ttgcccacat gcggtatgcc attcaacccc ggcattcccg
     6241 ccagttgtat ggcaagcgcc tccagccttc ccaggctacc aacaggtttg agtaacccgt
     6301 caatatgccg ttgtgcacgc gacatagcgg caggatcgat agcagggatc gtattcagta
     6361 aatcggcaag tgtttgcatg tcacgtctct ttatagcagt gccagcagga agaccagttc
     6421 accaagctct atagcagctc ccagcgtatc gccggtttgc ccgcccagcg tacgtttgag
     6481 aagctgaccg aggatgaaaa tcgccaccat cgtcaccacc atagcagcca caccatgcat
     6541 acccggcaat aatacggcag caaaaatagc ggctaagccg agtgtgacgc aggtttgtcg
     6601 cccatcaatt ttgccaatga acacattgcc aagcccttct tcccgggcat aacgatgacg
     6661 atacatcaat aatgcggcag tcccgcggct gaccgcacat gctgccgcca gcgatgcaag
     6721 gatcggttcg ccacgcagag ccaactcgct taataccaga atctttgcca gtacaacaaa
     6781 aatcaatgcc agaccgccat gggtgcctaa acggctatca cgcatgattt ccaacatgcg
     6841 atcgcggcta cgtgcagaaa atacgccatc acaggtatcg gcgaggccgt ctaggtggaa
     6901 tcccccggtc atcagcgcca gcacaagtac gctaaacagt gccgccagtg gtacgccaca
     6961 ccatgcctgc agcaccatga agaccagccc gctaatcgcg ccaagcaata atccaattaa
     7021 aggaaaagta ataataccgc gagaataatg ctcgaaatcc agtccctggg accagcgacg
     7081 cagtacaggc agacgcgtaa taaatgagag catcgcccaa aataatttac tcatttgatt
     7141 tttactccaa tacccgaaac caccagccat acttcatttg ccgcagcggc cagctgctga
     7201 tttacccgcc cggcaatatc acgaaaatgt cgtgccagac gactctccgg cacaatcccc
     7261 attcccactt cgttagtcac taatacaacc tttgcggggc aacgttggca ggcagcaatc
     7321 aacgactgaa tctcagcatt aatcgcctgt tccatcgcct gataatccca ttcatcaggg
     7381 tctttatcgc cgccataatc aaacaacaga ttagtcacca ttgtggtaac gcattccagc
     7441 aacacagctt catgcgggtt aatgtctgca tgaattaatt catcaagatg ttgccaacgc
     7501 tccaccgtgc gccagtgcgc cggacggcct tgccgatgat gttctatccg tgcagccatc
     7561 tcgtcatcaa ggatttgcga ggtggcgata tacagaacct gtgaagagtc cccaataagc
     7621 gcctctgcgt ggcgactctt cccgctccgt gcgccccccg tcaccaaaat catcataccg
     7681 actcctgatt caattgtatg atggtattca ttgaacagaa caatggatcc cccaccattg
     7741 ctgaccgcaa aacatttaca cgatcaatac tgcgcgtaat ataattaaaa atccagcatt
     7801 ctcaatccat tacgaaagaa gatggcattg tggaatagct agacttcctc gtgttaacgt
     7861 ccgacaaaat gcgtcattga cagcggtttc tgtggattga cacatttcat tcagataatg
     7921 aattaatgct actgccggac caaccagcaa acgggcattg tgttctgaaa tccaggctat
     7981 tgattcaacg tcagcgacag atctgcgctg taaaagaact tgtaaaacaa cgcaatagaa
     8041 ttatccggaa tggtggcgac tatgcactag aacaccacaa tttcgctctc tcggtaaggc
     8101 actgtcacag cattcaacag aatgtgactt gccagattta ttgtaagcgt cgtctcagca
     8161 ccgtctggca gatcctgaaa ttcctgagag aatagtggac accaaatatg gtggacgcta
     8221 tccatgaaat cattaaccgc agtgcgtaaa aaaagcccta attatcccgt tgagttcaaa
     8281 atcaaaatgg ttgaactctc gcatcgacca gagatctccg tagcgcaact cgctcgtgag
     8341 catgggatca acgataattt gctgttcaag tggcgccagt actggcgcga aggaaaacta
     8401 cgtcctcctt caacaacaga aaacaacgtg cctgagctgc tcccgataac acttgatgcc
     8461 gaagatgttg tccctacaac ctccccccgg tcacaacctg tagctgctgc gacacctgaa
     8521 tcactcaata tcagctgtga agtgacgttc cggcacggat cactccgtct gaatggtgcc
     8581 atcagcgaaa atatcctgaa cctgctgata cgggagctca aacgttgatc ccattaccat
     8641 cagggacaaa gatctggctg gtcgctggca tcaccgatat gagaaacggc ttcaacggcc
     8701 tggcggcaaa ggtgcagacg acgctgaaag acgatccgat gtcaggtcac gtttttatct
     8761 tccgtgggcg taatggcagt caggtaaagc tcctctggtc taccggcgat ggactgtgtc
     8821 tgctgaccaa acggctggag cgcggccgct tcgcctggcc gtcagcccgg gatggcaaag
     8881 tgttcctcac accggcacag ctggcgatgc tgctggaagg tatcgactgg cggcagccta
     8941 aaagactgct tacgtccctg actatgttgt aagcctcttt atcctggtcg acgctgaatg
     9001 agcctggtaa tatacccggt atgagcagct cacttcctga cgatatcaat gcactgaaac
     9061 gtctccttgc cgaacaggag gcgctgaacc gtgccctgca ggaaaagctg aacgagcgtg
     9121 aacgcgaaat agaccatctg caggcacagc tggataagct gcgccggatg aacttcggca
     9181 gccgctcgga aaaagtctcc cgtcgtatcg cacagatgga agctgacctg aaggcacttc
     9241 agaaagaaag tgataccctt accggtcggg ttgacgaccc ggccgtgcag cgcccgctgc
     9301 gtcaaacccg cacccgcaaa ccgttccccg aatcactccc ccgcgatgaa aaacggctgc
     9361 tgccggcagc gtcatgctgc ccggaatgtg gaggctcgct gagctatctg ggtgaggatg
     9421 ccgccgaaca gctggagctg atgcgcagcg ccttccgggt tatccggact gtacgtgaaa
     9481 agcatgcctg tactcagtgc gatgccatcg tgcaggcccc cgcgccttca cggcccatcg
     9541 agcggggtat cgcaggaccg gggctgctgg cccgcgtgct gatctcaaag tatgcagagc
     9601 acaccccgct gtaccgccag tctgaaatgt acggccgcca gggcgtggag ctgagtcgtt
     9661 cactgctgtc gggctgggtg gatgcatgct gccggctact gtcaccgctg gaagaagcgc
     9721 ttcaggacta tgtgctgact gacggtaagc tccatgctga tgacacgcct gtcccggtgc
     9781 tgttgccagg caataagaaa acgaagaccg ggcggttatg gacctacgtt cgtgacgacc
     9841 gtaacgccgg gtcaacgctg gcgccggcgg tgtggttcgc ttacagcccg gacagaaaag
     9901 gcatccatcc gcagacccat cttgcggggt tcagtggtgt actgcaggcg gatgcatacg
     9961 ccgggttcaa cgagctgtac cgggatggcc ggataacgga agccgcctgt tgggctcacg
    10021 cccgccgtaa aatccacgat gtgcacgttc gcaccccgtc agccctgacg gaggaagcgc
    10081 tgaaacggat cggcgaactg tacgccatcg aggcagagat aaggggaatg acggcggagc
    10141 agcgccttgc cgaacgtcag ttgaaaacga aaccgctgct gaaatccctg gaaagctggc
    10201 tgcgtgaaaa gatgaaaacc ctgtcgcgac actcagaact ggcgaaagcg ttcgcatacg
    10261 ccctgaacca gtggccggcg ctgacgtact atgcagatga tggctgggct gaggcggaca
    10321 ataacatcgc tgaaaatgcg ttgcggatgg tcagtctggg ccgcaaaaac tacctgttct
    10381 tcggttcgga tcatggagga gagcggggag cgctgctgta cagcctgatc gggacgtgca
    10441 aactgaacgg agtggagcca gaaagctacc tccgctatgt ccttgacgtc atagccgact
    10501 ggccgataaa ccgggtcggc gaactgctcc cctggcgcgt agcactgccg actgaataac
    10561 acatccccgt caatacggtt cttgctgcac gcttacgatt tattagcgcc accagatgtt
    10621 taaccgggta accacgaccc agtcggggat atattccatc ttctcgctga catcatcaag
    10681 aataaaaagg tttatcacac tcagagcaac gttcttcagc aggcgggata attcttcaac
    10741 atttaaaaga tgtgttatta aaggcataca aactggacgt attgtatttt cttttggtgg
    10801 ttgacctaag gtagcagttt atcctgatgc gctgagattt ctgtaatatc tacgtcaaca
    10861 ttctcctcga atagaaaatg cagcctcggc aagtgattca cattttttaa caaaacgttg
    10921 ttagcgtgcc attttcagaa gctctccaag aaagcgaatt cttttctcaa attctgcatt
    10981 ctcagacaca gctttctact ccatggcaat ggcccaaata cgaagttgct caggatcatt
    11041 agtggtgctg agaagttaaa taaccatgcg gagtaccact cgtcatcacg tatttatata
    11101 tcatgaagct attgttgtgt tttgtacatc agtagatgta ttctgatata ctccttttgc
    11161 tagacataac ctttcacctg cttgcaaagc ttctgtgttc tgacattgcc aaattgttgc
    11221 aattctgtat ccagccttct ttcagtcata gcttcgggcc acgataagac tcactgatct
    11281 gaccctgatt cctcttgcag actttataga ccaattaaaa tgcagtttct gcaggtcaac
    11341 gtctgaccat cattgtcatc actctggcca ttagagtaac cttctgcatt catccttttg
    11401 taaaaagttt atgtcagtat cagcaattaa ccggacctga tactgatatg agtcttaccg
    11461 catatacggt caatttcagc aattaattac attatccacg ccaaagtgtt tgtcatcgca
    11521 atgatggtac cttctttcag acaccatttt ttcaactccg ttttccacgg accgcactct
    11581 tatgtcaaga gtgcggtgat cctacccacg taatatggac acaggcctaa gcgaggttct
    11641 ggttttcaaa ttgttccgga ctgaggccgc cacaccaact gtgccgccgc caccgattgt
    11701 aatcacattc gatataatta aacaccgttg cccgcattat ttcccggctg ataaagtgtt
    11761 ctccatggat acattccact ttcagcgaat gaaagaagct ttccacgcag attatgccgc
    11821 ttcagttgcg cctgataatc tgctgaacag tactggcctc cacggtccgt gtgaacgata
    11881 acgttccggg gcctcttacg ccgcca
//
LOCUS       DAJOWB010000131        11375 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJOWB010000131 DAJOWB010000000
VERSION     DAJOWB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.131"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            7..747
                     /gene="ydeO"
                     /locus_tag="OHO85_003963"
     CDS             7..747
                     /gene="ydeO"
                     /locus_tag="OHO85_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCQ2673268.1"
                     /translation="MFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYERHFKEI
                     GFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSEL
                     LLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLL
                     KKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGN
                     SPKRVSKEYRCQSHTGMNTGNTMSALAI"
     gene            1149..2831
                     /locus_tag="OHO85_003964"
     CDS             1149..2831
                     /locus_tag="OHO85_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCQ2673269.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            2883..4040
                     /locus_tag="OHO85_003965"
     CDS             2883..4040
                     /locus_tag="OHO85_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCQ2673270.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CSFLKEHEFLAGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(4006..4128)
                     /locus_tag="OHO85_003966"
     CDS             complement(4006..4128)
                     /locus_tag="OHO85_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673271.1"
                     /translation="MTTHPTGIYVGCFVKVIRRRLRLELRGRVINYSPFVLQHP"
     gene            4331..6016
                     /locus_tag="OHO85_003967"
     CDS             4331..6016
                     /locus_tag="OHO85_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCQ2673272.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
     gene            6054..8426
                     /locus_tag="OHO85_003968"
     CDS             6054..8426
                     /locus_tag="OHO85_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCQ2673273.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINASGKKTNHTIYHKGKGRLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFANQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            8483..11266
                     /locus_tag="OHO85_003969"
     CDS             8483..11266
                     /locus_tag="OHO85_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCQ2673274.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         3366 a         2481 c         2452 g         3076 t
ORIGIN      
        1 tctgttatat ttattcatca tgccttcaac gctaacattt tagataaaga ttacgccttc
       61 tctgacggcg agatcctgat ggtagataac gctgttcgta cgcattttga accttatgag
      121 cggcatttta aagaaatcgg atttaatgaa aataccatta aaaaatatct acaatgcact
      181 aacatccaga cagtgacggt gcctgttcct gcgaagtttt tacgtgcttc aaatgtaccg
      241 actggattgc ttaatgaaat gattgcttat ctcaactcgg aagaacgcaa tcatcataat
      301 ttttcagaac ttttgctttt ttcttgcctg tctatttttg ccgcatgcaa aggtttcatt
      361 acactattaa ctaacggtgt gctatccgtt tccggaaaag tgagaaatat tgtcaacatg
      421 aagctggcgc acccatggaa gctgaaagat atttgtgact gcctgtacat cagtgaaagc
      481 ctgttgaaga aaaaactaaa gcaagagcaa acgacattct cacagattct attagatgcc
      541 agaatgcagc acgcaaaaaa tttgatacgc gtagaaggtt cagtcaataa aattgccgaa
      601 caatgtggtt atgccagtac atcttatttt atttatgcgt tccgcaaaca tttcggcaac
      661 agtccgaaga gagtttctaa ggagtaccgt tgtcaaagtc acacgggtat gaatacgggc
      721 aacacgatga gtgctttagc tatttgatta tttgctaacg agtagtcaac cacgcacgct
      781 acgtaagaat tatatggggc agccattccc tgccccgcgt tgtttttagg cgatatattt
      841 attgaaataa ataagtgaca tccatcacat atttatgcac ttgcataacc tgttgcatga
      901 ttatttatga tctcaattcg gcattttgtc agtaaaatgc aataatttat taaatatcaa
      961 taaattagtt gtttatcgtc gagaaattac ttaatagaac agaaagtaat gtcaacgctt
     1021 tatggactgt tatttccctt tttttagcta aatctgctat ctctttatgt gactaacttc
     1081 acttacaccc acttatttct cttcgtaaaa ttacttcgga attaagtaca ataagaagag
     1141 gaacatttat gaagtctgca ttaaagaaaa gtgtcgtaag tacctcgata tctttgatac
     1201 tggcatctgg tatggctgca tttgctgctc atgcggcaga tgatgtaaag ctgaaagcaa
     1261 ccaaaacaaa cgttgctttc tcagacttta cgccgactga atacagtacc aaaggaaagc
     1321 caaatattat cgtactaacc atggatgatc ttggttatgg acaacttcct tttgataagg
     1381 gatcttttga cccaaaaaca atggaaaatc gtgaagttgt cgatacctac aaaataggga
     1441 tagataaagc cattgaagct gcacaaaaat caacgccgac gctcctttca ttaatggatg
     1501 aaggcgtacg gtttactaac ggctatgtgg cacacggtgt ttccggcccc tcccgtgccg
     1561 caataatgac cggtcgagct cccgcccgct ttggtgtcta ttccaatacc gatgctcagg
     1621 atggtattcc gctaacagaa actttcttgc ctgaattatt ccagaatcat ggttattaca
     1681 ctgcagcagt aggtaaatgg cacttgtcaa aaatcagtaa tgtgccggta ccggaagata
     1741 aacaaacgcg tgactatcat gacaacttca ccacattttc tgcggaagaa tggcaaccgc
     1801 aaaaccgtgg ctttgattac tttatgggat tccacgctgc aggaacggca tattacaact
     1861 ccccttcact gttcaaaaat cgtgaacgtg tccccgcaaa aggttatatc agcgatcagt
     1921 taaccgatga ggcaattggc gttgttgatc gtgccaaaac acttgaccag ccttttatgc
     1981 tttacctggc ttataatgct ccgcacctgc caaatgataa tcctgcaccg gatcaatatc
     2041 agaagcaatt taataccggt agtcaaacag cagataacta ctacgcttcc gtttattctg
     2101 ttgatcaggg tgtaaaacgc attctcgaac aactgaagaa aaacggacag tatgacaata
     2161 caattattct ctttacctcc gataatggtg cggttatcga tggtcctctg ccgctgaacg
     2221 gggcgcaaaa aggctataag agtcagacct atcctggcgg tactcacacc ccaatgttta
     2281 tgtggtggaa aggaaaactt caacccggta attatgacaa gctgatttcc gcaatggatt
     2341 tctacccgac agctcttgat gcagccgata tcagcattcc aaaagacctt aagctggatg
     2401 gcgtttcctt gctgccctgg ttgcaagata agaaacaagg cgagccacat aaaaatctga
     2461 cctggataac ctcttattct cactggtttg acgaggaaaa tattccattc tgggataatt
     2521 accacaaatt tgttcgccat cagtcagacg attacccgca taaccccaac actgaggact
     2581 taagccaatt ctcttatacg gtgagaaata acgattattc gcttgtctat acagtagaaa
     2641 acaatcagtt aggtctctac aaactgacgg atctacagca aaaagataac cttgccgccg
     2701 ccaatccgca ggtcgttaaa gagatgcaag gcgtggtaag agagtttatc gacagcagcc
     2761 agccaccgct tagcgaggta aatcaggaga agtttaacaa tatcaagaaa gcactaagcg
     2821 aagcgaaata actaaacctt catgcggcgg atttttccgc cgccttattg agcgagatag
     2881 cgatgcacgt tacagccaag ccctccagtt ttcaatgtaa tctcaaatgt gattactgtt
     2941 tttaccttga aaaagagtcg cagtttactc atgaaaaatg gatggatgac agcactctga
     3001 aagagttcat caaacaatat atcgcagcgt ctggcaatca ggtctatttt acctggcaag
     3061 gcggtgaacc cactctggct ggcctggatt ttttccgtaa agttattcac tatcaacaac
     3121 gctatgcagg ccaaaaacgt atttttaatg cattacaaac gaatggcatt ttattgaata
     3181 atgaatggtg ttcctttctc aaagaacatg aatttctggc tggtatctcg atcgatggcc
     3241 cccaggagtt acatgaccgt tacagacgca gtaattcagg taacggtact tttgcaaaag
     3301 tgatagcagc catcgagcgt ctgaaatcat atcaagtaga gtttaatacg ttaaccgtca
     3361 ttaataacgt taatgtccat taccctcttg aggtttatca ttttttaaaa tctatcggca
     3421 gtaaacatat gcaatttatc gaattgctag aaaccgggac gccgaatatt gatttcagtg
     3481 gtcatagtga gaacacattc cgtatcattg atttttctgt gcctcccacg gcttatggca
     3541 agtttatgtc aaccattttt atgcaatggg ttaaaaacga tgtgggtgaa attttcattc
     3601 gtcagtttga aagctttgtc agccgttttt tggggaatgg gcatacaagt tgtattttcc
     3661 aagagtcctg caaggataat ctggttgttg aaagtaatgg agacatttac gaatgcgacc
     3721 attttgtcta tccacagtac aaaattggaa acattaataa atctgaactc aaaacgatga
     3781 acagtgtaca actgacagcg caaaaaaaac ggatttcagc gaaatgtcag caatgtgcat
     3841 ataaacctat ctgcaatggc ggttgtccta agcatcgtat tactaaagta aacaatgaga
     3901 ctgtttctta tttttgcgaa ggttataaaa tccttttttc aaccatggta ccttatatga
     3961 acgccatggt agagttagct aagaacagag taccgcttta tcacattatg gatgttgcaa
     4021 aacaaatgga gaataattaa taacacgccc tcttagctcc agtcttagcc tccgtcttat
     4081 gaccttaaca aagcacccga cataaattcc tgtcgggtgt gtcgtcatca tcatcttccc
     4141 ttgcgaaaat cctggcatac gtggtatgcg tcagggcatt agcaataccc ttgattatca
     4201 atagcataac cataaccact gaaaattaaa aatttctata tatttcatat tgttaaatga
     4261 cacttaaata aatttcatca gtaaatcatt gccatagata atcattggat ggcacaatca
     4321 attttcgcta atgataacca ttcccattac gttttgtatg ttgatagcaa agtatctctg
     4381 tctgctgaag cccttttggc tgcgtaagaa taataaaact tcagtgttgt taatcattat
     4441 tatcctcgcc atgatccttg gcgtggttaa aattcaggtc tggttaaacg actggaataa
     4501 tgattttttc aatgcgctaa gccagaaaga aaccgataag ctttggcagc ttgtcctttg
     4561 gttccctgcc ctgctgggga tctttgtatt aatatccgta aacaaaacat ggttaattaa
     4621 actgctgact attcgctggc gcgaatggtt aacagattac taccttaatc ggtggtttgc
     4681 agataaaaac tactacttca cgcagatcta cggtgagcat aaaaatacag ataaccctga
     4741 ccaacgtatc gctgaagaca ttctcttact gatcagcaaa acgctcagcc tttcatttgg
     4801 cttcatccag tcacttagca tgctgatcac ctttaccgtt attctttggc aaagcgcggg
     4861 tacgctctca ttcactgttg gtggaacaga atggaatatc caagggtata tggtctatac
     4921 cgttgtgctc attgtgatcg gtggaacttt atttacccat aaggtgggta aacgtattcg
     4981 tccgcttaat gtggaaaaac aacgtagcga agcgacattt cgaactaatc ttgtgcagca
     5041 taataagcag gcagaactga ttgcgctaag caatgctgag tctctacaac gccaggagtt
     5101 gcgcgacaat tttcatacca ttaaagagaa ctggcatcgt ttaatgaatc gtcagcggtg
     5161 gcttgattac tggcaaaata tttattcgcg ctcgcttagc gttcttccct actttctgtt
     5221 attgccgcag tttattagcg ggcagatcaa tctgggcgga ctaatgaaat cgcgccaggc
     5281 atttatgcta gtatcgaaca atttaagctg gtttatttat aaatatgacg aacttgctga
     5341 gctggctgcg gttatcgatc gcttgtatga gttccatcaa ctcactgaac agcgccctac
     5401 gaataagcct aaaaattgcc aacatgcggt acaagtggct aatgcgagta ttcgtacgcc
     5461 tgataataag atcatattag agaacttgaa ctttcatgtt tcgccaggca aatggctatt
     5521 actgaaaggc tactctggtg cgggaaaaac cacactgctt aaaacattat cccactgctg
     5581 gccgtggttt aaaggtgata tttcttctcc tgctgacagt tggtatgtgt cacaaacacc
     5641 gttaatcaaa agcggcttac tgaaagagat tatttgtaaa gcacttcccc tgcccgtaga
     5701 cgataaatcg ttgagcgaag tactgcatca ggttggtctg gggaaattgg ctgcgcgtat
     5761 tcatgaccac gatcgctggg gagatattct ttccagcggc gaaaaacaac gtatcgccct
     5821 ggcacgatta attttacgac gtccgaaatg gatatttctt gacgaaacta cctctcatct
     5881 tgaggaacaa gaggctatac gcttactgcg tttagtgcgt gaaaaactac ccacaagcgg
     5941 cgtcattatg gttacacatc aacccggtgt ctggaacctg gctgatgata tttgtgacat
     6001 tagcacggtt ttataacaac aataaaagcc ctgctgtacg gagatataaa taaatgaagc
     6061 gagttcttat tcctggcgtc attttatgtg gcgctgatgt ggcgcaggcc gtcgatgaca
     6121 aaaacatgta catgcatttt tttgaagaga tgacggtcta tgctcctgtc cctgtacccg
     6181 taaacggcaa cacgcattac accagtgaaa gcatcgagcg tttaccgacc gggaatggca
     6241 atatcagcga tctgctgaga accaaccctg cggtacgcat ggattcaacg caaagtacct
     6301 cgttgaacca gggagatatt cgccctgaga aaatctctat tcacggtgcg tcgccctacc
     6361 agaatgccta tttgattgac ggtattagtg caactaataa cctgaaccca gcgaatgagt
     6421 ccgatgccag tagtgcaacc aatattagcg ggatgtcaca ggggtattat cttgatgtca
     6481 gcttactgga caatgtgacg ctttatgaca gttttgtgcc ggttgaattt ggtcgcttca
     6541 atggcggggt aattgatgca aagatcaaac gcttcaacgc tgatgatagc aaggtgaaat
     6601 tgggttatcg cactacgcgt tcggactggt taacatcgca tatcgatgag aataacaaga
     6661 gcgcatttaa tcaaggctct tcaggaagta cttattattc cccagatttt aaaaagaact
     6721 tttatacctt gtcgtttaat caggaactcg ctgataactt cggcgttacc gccggtttat
     6781 cgcgccgcca gtctgatatc acccgcgcgg attatgtttc gaatgacggc attgtcgccg
     6841 gtcgggcaca gtataaaaac gttatcgata ctgcattgag caaatttacc tggtttgcca
     6901 gcgaccgctt tacccacgat ttaaccttaa aatataccgg ctccagccgt gattataata
     6961 ccagcacctt cccgcagtct gatcgcgaaa tgggtaataa atcctatggt ctggcatggg
     7021 atatggatac gcagctcgca tgggccaaac tgcgtaccac cgttggttgg gatcatatta
     7081 gtgattatac ccgtcacgat catgacatct ggtacaccga actttcatgt acatatggtg
     7141 atattacagg gcgttgcacc cgtggcggat taggacacat ttcccaggct gtagataatt
     7201 acaccttcaa aacacgcctg gactggcaaa aattcgccgt gggtaatgtt tcgcatcaac
     7261 cctacttcgg cgcggaatac atctattccg atgcgtggac tgaacgccat aaccagtctg
     7321 aatcctatgt gattaatgct tccggaaaga aaactaacca taccatttac cataaaggta
     7381 aaggccgcct gggaattgac aactacacgc tgtatatggc ggatcgcatt agctggcgta
     7441 atgtgtcatt aatgcccggc gtgcggtatg actatgacaa ctatctgtca aaccacaata
     7501 tctccccgcg ctttatgacg gaatgggata tttttgctaa tcaaacctca atgattacag
     7561 caggttataa ccgttactat ggcgggaata ttcttgatat gggattacgt gatatccgca
     7621 atagctggac ggaatcggta tcaggtaata aaactctgac gcgttatcag gatttgaaaa
     7681 cgccttataa cgatgaactg gcaatgggat tgcagcagaa aatcggtaag aacgttattg
     7741 cgcgtgcaaa ctatgtttac cgtgaagcgc atgatcaaat cagcaaaagc agtcgtaccg
     7801 acagcgcgac taaaaccacc attactgaat ataacaacga tggcaaaacc aaaacgcatt
     7861 cgttcagcct cagttttgaa ctggccgaac ccctgcatat ccgccaggta gatattaacc
     7921 cacaaattgt ctttagctat atcaagagca agggcaactt gtcgttaaac aatggttatg
     7981 aggagagcaa taccggtgat aaccaggtgg tttataacgg taatctggtc tcttacgata
     8041 gcgttccagt ggcagatttt aataacccat taaagatctc cttaaacatg gatttcacgc
     8101 atcaaccgag cgggttagtg tgggcgaata cgctggcctg gcaagaagcg cgtaaagctc
     8161 gcattatcct tggtaagaca aatgcgcaat acatcagcga atattcagat tacaagcagt
     8221 atgttgacga aaaactggat agcagcctga cctgggacac ccgcttgtcc tggacgccac
     8281 aatttctgaa acaacaaaac ctgacgatca gtgccgatat tctcaatgta ctggatagca
     8341 aaaccgctgt tgatacaacg aacaccggtg tggcgaccta cgccagtggc cgtactttct
     8401 ggcttgatgt cagcatgaaa ttttaatagt tcggatttta cttaaccggg taaattcgcc
     8461 cggttttcgc atggagataa ttatgagaaa cctctgtttc ttactgacgt tagtggcaac
     8521 tctgttgctc cccgggcggc tgattgccgc cgccttaccg caggatgaaa agttaattac
     8581 cgggcaactg gacaatggct tgcgatatat gatttatccg catgctcatc caaaggatca
     8641 ggtaaattta tggctgcaaa ttcataccgg ttcattgcag gaagaagaca atgagcgcgg
     8701 cgtggctcat tttgtagaac atatgatgtt taacggcaca aaaacatggc cgggtaataa
     8761 agtcatcgaa acatttgagt caatgggcct gcgttttggt cgcgatgtta atgcctatac
     8821 cagctatgac gaaacggtgt atcaggtgag tttgccgact acgcagaaac aaaatctgca
     8881 acaagtgatg gcaatcttca gtgaatggag taatgccgca acctttgaaa aactcgaagt
     8941 agacgctgaa cgtggcgtaa ttactgagga atggcgtgcc catcaggatg cgaaatggcg
     9001 cacctctcag gcgcgccgcc ctttcctgct ggcaaatacc cgtaatttag accgtgaacc
     9061 tatcggcctg atggatactg tcgccacggt cacaccggca caattgcgcc aattttatca
     9121 acgctggtat caaccaaata atatgacctt tatcgtggtc ggcgatatcg acagtaaaga
     9181 agcgctggcg ctgataaagg ataatttaag taagcttccg gctaacaaag cagctgaaaa
     9241 tcgcgtctgg ccgacaaaag ccgaaaacca cctgcgcttt aatatcatta atgataaaga
     9301 aaaccgggtg aacggcatcg cactctatta tcgcctgcca atggtacaag tgaacgatga
     9361 gcaaagcttt gtcgaacaag ctgaatggag catgttagtt cagctgttca atcaacgtct
     9421 gcaggaacgc atacagtcgg gcgagttgaa gactatttct ggcggcactg cgcgcagcgt
     9481 taaaattgca cccgattatc agtcgctgtt tttccgtgta aatgcacgag acgataatat
     9541 gcaggatgct gcgaatgcat taatggcaga gttggcaacc attgatcagc atggtttttc
     9601 tgctgaagaa ctcgatgatg tcaaatctac ccgcctcacc tggctgaaaa atgcggttga
     9661 tcagcaagct gaacgtgatt tacgtatgct gaccagtcgc ctggcatcca gctcattaaa
     9721 taatacgccg ttcttgtcgc cggaagagac atatcaactt tcgaaacgtc tgtggcagca
     9781 aattaccgtg caaagtctgg cggaaaaatg gcagcagtta agaaagaacc aggacgcatt
     9841 ttgggagcaa atggtaaaca atgagcttgc cgccaaaaaa gcattgtctc ctgcggctat
     9901 cctggcgctg gaaaaagagt acgccaacaa aaagctggcg gcttacatct tcccaggcag
     9961 aaatttatcg ttaacagtag acgctgaccc acaggcggaa attagcagca aagaaacgct
    10021 ggctgagaat ctgacatcat taacactttc caatggtgcc agggttattc tggcaaaatc
    10081 cgcgggtgaa gagcaaaagc tacaaattac tgccgtatcg aataaaggcg atttaagttt
    10141 ccctgcgcag caaaaatcac ttatcgcgct ggcaaataaa gcagtaagcg gaagcggcgt
    10201 tggtgagctc tcctcttcca gcctgaaacg ctggagtgcg gaaaattcgg taaccatgag
    10261 cagtaaagtc agtggcatga atacgttgct ctccgttagc gcgcggacta ataaccctga
    10321 acctggtttt cagttgatta accagcgaat cacccacagc acgattaacg acaatatttg
    10381 ggcatcgcta caaaatgctc aaattcaggc gttgaaaacg ctcgaccagc gtccagcgga
    10441 gaaattcgcc cagcagatgt atgagacgcg ctatgctgat gaccgcacga aattactgca
    10501 agaaaatcag attgtacagt ttactgccgc agatgcgctg gctgccgatc gccagttgtt
    10561 ttcatctcca gcggatatca cgtttgtcat tgtcggtaat gtctcagaag acaaactcgt
    10621 ggcgttaatt acgcgttact taggatcaat caaacactct gattcgccat tagccgcagg
    10681 taaaccatta actcgcgcga cggacaacgc atcggttact gtaaaagaac aaaatgaacc
    10741 tgtggcacag gtttcacagt ggaagcgtta tgattcccgg acacctgtta atctggcgac
    10801 gcgtatggcg ctcgatgctt ttaacgtcgc actggcaaaa gatctacgtg ttaatattcg
    10861 tgaacaggca tctggagcat acagcgtttc ttctcgcctc tcggttgatc ctcaggccaa
    10921 agatatcagt catttgctgg cttttacttg tcaaccagaa cgacatgatg aactgttaac
    10981 gttagcgaat gaagtgatgg ttaagcgtct ggctaaaggg atcagtgagc aagaactgaa
    11041 tgaataccag caaaacgttc agcgcagcct cgatatccaa cagcgtagcg ttcaacaatt
    11101 agcgaacact attgtaaata gtcttattca atatgacgat cctgcagcat ggactgagca
    11161 ggagcaattg ttgaaacaaa tgacggtaga gaatgttaac actgccgtta aacaatatct
    11221 ttctcatccg gtaaatactt ataccggagt attattgcca aaataataac agccccgtca
    11281 acacatcgtt ggcggggatt ttagcaatat tcgctatttt tatgtaataa ttttataaat
    11341 gcgttcaaaa taataatcaa gtactaatag tgata
//
LOCUS       DAJOWB010000132        11368 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJOWB010000132 DAJOWB010000000
VERSION     DAJOWB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.132"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..832
                     /locus_tag="OHO85_003970"
     CDS             <1..832
                     /locus_tag="OHO85_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCQ2673275.1"
                     /translation="SHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIAD
                     DHPTNRLLLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRK
                     LREQNSSLPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAP
                     QYRHLDIEALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRI
                     HGAANILNLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND
                     "
     gene            complement(888..2033)
                     /gene="yfdE"
                     /locus_tag="OHO85_003971"
     CDS             complement(888..2033)
                     /gene="yfdE"
                     /locus_tag="OHO85_003971"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCQ2673276.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(2107..3051)
                     /gene="yfdV"
                     /locus_tag="OHO85_003972"
     CDS             complement(2107..3051)
                     /gene="yfdV"
                     /locus_tag="OHO85_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCQ2673277.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(3121..4815)
                     /gene="oxc"
                     /locus_tag="OHO85_003973"
     CDS             complement(3121..4815)
                     /gene="oxc"
                     /locus_tag="OHO85_003973"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCQ2673278.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTGMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(4869..6119)
                     /gene="frc"
                     /locus_tag="OHO85_003974"
     CDS             complement(4869..6119)
                     /gene="frc"
                     /locus_tag="OHO85_003974"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCQ2673279.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(6632..7264)
                     /locus_tag="OHO85_003975"
     CDS             complement(6632..7264)
                     /locus_tag="OHO85_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCQ2673280.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            7560..7835
                     /gene="ypdI"
                     /locus_tag="OHO85_003976"
     CDS             7560..7835
                     /gene="ypdI"
                     /locus_tag="OHO85_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HCQ2673281.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(7912..8154)
                     /locus_tag="OHO85_003977"
     CDS             complement(7912..8154)
                     /locus_tag="OHO85_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCQ2673282.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            8507..9427
                     /gene="lpxP"
                     /locus_tag="OHO85_003978"
     CDS             8507..9427
                     /gene="lpxP"
                     /locus_tag="OHO85_003978"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCQ2673283.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            9783..9854
                     /gene="ypdK"
                     /locus_tag="OHO85_003979"
     CDS             9783..9854
                     /gene="ypdK"
                     /locus_tag="OHO85_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCQ2673284.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(9919..11157)
                     /gene="alaC"
                     /locus_tag="OHO85_003980"
     CDS             complement(9919..11157)
                     /gene="alaC"
                     /locus_tag="OHO85_003980"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCQ2673285.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT         3139 a         2730 c         2469 g         3030 t
ORIGIN      
        1 aagtcatcca ggcataggaa caacatttac gatcacaatc ccggtagaaa ttagccagca
       61 agtggcgact gtcgaggcaa aagcagaaca ccccatcaca ctacctgaaa agttgagcat
      121 attaatcgcg gatgatcatc cgaccaacag gctattactc aaacgccagc taaatctatt
      181 aggatatgat gttgatgaag ccactgatgg tgtgcaagcg ctacacaaag tcagtatgca
      241 acattatgat ctgcttatta ctgacgttaa tatgccgaat atggatggtt ttgagttgac
      301 tcgcaaactc cgtgagcaaa attcttcctt acccatctgg gggcttacag ccaacgcaca
      361 ggctaacgaa cgtgaaaaag ggttaagttg cggcatgaac ttatgtttgt tcaaaccgtt
      421 gaccctggat gtactgaaaa cacatttaag tcagttacac caggttgcgc atattgcacc
      481 tcagtatcgc caccttgata tcgaagccct gaaaaataat acggcgaacg atctacaact
      541 gatgcaggag attctcatga ctttccagca tgaaacgcat aaagatctac ccgctgcgtt
      601 tcaagcacta gaagctggcg ataacagaac tttccatcag tgtattcatc gcatccacgg
      661 tgcggctaac atcctgaatt tgcaaaagtt gattaatatt agccatcagt tagaaataac
      721 acctgtttca gatgacagta agcctgaaat tcttcagttg ctgaactctg taaaagaaca
      781 cattgcagag ctggaccagg agattgctgt tttctgtcag aaaaatgact aaatagcggc
      841 tcccacaatg ttcaaatgtg ggagctattt accagcacat cttacattta tgatgagaac
      901 tcctggcgaa tttgttcccc atgctggtcg agcgttgccg ctcccggcat aacatgcggg
      961 tccgcgcagc cgctgatttt tatcggatta cccggcatca ttattccccc ggcttcaatc
     1021 aacatatttc ttgcctgagt ttgtggcaat ttaatggcct cagccacact taataacggc
     1081 gcgacgggta caccaacttc atgtattctg gctaaccaaa cttcagctgc ctgcgttttt
     1141 aacgtccgct caatatattg tttaagaata gcctggtttt gtacgcgtaa aatattgctg
     1201 ctaaatcggg gatcattaac cagttccgta agctccagtg cctggcataa cgcagaaaaa
     1261 agcttgtcat taccacaaca aatcgtaatc ggcttatcct gagtattgaa aacatcaaaa
     1321 ggtgccatgt agggatggcg atttcccaga cgttgtggtg acttccctgt cgcgatatat
     1381 gccatcagac catgctccag aaaactcagc gtggcatcaa acatcgctat atcgacatgc
     1441 gcccctctct ggctcttttc gcggccataa agtgcactca ctattccgct gaataaatag
     1501 acaccgccgc atagatccgc aagagatgta ccaacgcgca ctggcggagc atcaggatat
     1561 cctgtttcca tcattatccc gctcattgcc tgaatgatgg tatcgtaggc aggagcatct
     1621 tttagcggac cggtatgtcc gaaacctgac gatgaagcat atatgaggcg cgggttgatt
     1681 tcttgaagcg tttcccatga aaaccccagt ttttccattg tacctgggcg aaaattctca
     1741 gctaatacat cagcttgttt gagcatattt ataaatatac ttttatcgtg atcattcttt
     1801 aaatcaagaa ccacactctc tttgccatga ttaataaaac tgtaatagag tgactgtcca
     1861 tccacatagg gaccaaatgt gcgggtatca tcaccatgac ccggcggctc aactttaatt
     1921 acccttgcgc ccatattaca aagaagttga gttccgaaag gtccattaag gacatgtgtc
     1981 atatcgataa ctaataagcc ttcaaacggc cctttgcttt cattatttgt catttgctta
     2041 tgccttgaaa aaagccgaag gtgaaaagac acttcggcat ttatgtaaaa tcaataacat
     2101 gattaattat gaaaccagtc gactgacata aatccacaag ggagccgtga cgacaaagcc
     2161 cagtacgctc acagccaatg acgctgtacc agtacgggtg tagacattaa accggctggc
     2221 aataatgatt ccggagaatg ccggcggtaa tgcgcctgcc agtaccatca tctgcagatg
     2281 ttcgctgttc aaatgacatg ccataccaac gagaagcagt gccagtggca tcagaatcag
     2341 cttcaggaag gtgttataag caatttcggc actgaactcg aatttatgtg cagccagagt
     2401 caaccccgca gcgaataccg ctacccctga gttagccttc gcaatcagat taaaggttgg
     2461 gtcccatgct gccggaattt ttaccccaac caacaccagg atcgttgcca gaacaggtgc
     2521 ccatactact ggctcctttg ccgcagaaat taatgcgctc agattactat tcttcttacc
     2581 atccgctcct gaggaaggat tcagcaaata cagaccaata ggaatagtaa ttgcgttaac
     2641 aataatagaa ataattgcta ccactaaacc tgttgatacg gaatcaccat aaataggatc
     2701 gagaactgca aaccccagga atccaatggt aggtgaaccc gcaattaatg cacatacagc
     2761 tgcttctgca tgggtacgtt taaaaaattt gtagcaacca aaccaggaga aaaagaaaca
     2821 tccgacaata accacaagtg atacaagggt cagacgagtg tccgcaaaaa tcatttccct
     2881 gtttgcccgg gtaatagaaa caaatagagc cgcaggaaga gcgtagttta ataccagttt
     2941 attaaatgcg cgggcttgat cttctgaaaa agtttctcgt ctgccgctaa aataacccaa
     3001 cagcatgatt acgataatag gcaataaatc gccaataaaa aatgttagca taatagtatt
     3061 tccgataagg gtcccctcac catcgcataa tgagttggca agaggagacg atgtcagtat
     3121 ttaattacca gcgacttgtt ttgggttaag tttggtaata tggccacttt cagttcctgc
     3181 tgcagggtcg atgaccacat taataatggt cggtttgcgc gactgaatac cggtggttaa
     3241 agcatgacga agttcatctg tcgtggtgac gttatagcca acgccacgaa acgcatccat
     3301 taatttgtca taccttgcat ggtgcaacag atccgttggt gatggtgcac cagcgccact
     3361 gagatcaaca ccgtctcctc tgtagatgcc gccattatta aaaataacga tcgtcaccgg
     3421 caggttatat cgacaaatcg tttcaatttc catcccactg aaaccaaaag cactatcacc
     3481 ttcaatggcg acaaccggag aaccagaagt cacgctagca ccgatggcat agcccatacc
     3541 gatgcccatg acaccccagg taccacaatc cagacgacga cgtggtttat acatatcaat
     3601 aatatttcgt gcattatcca gggtatttgc accttcatta actaaataaa tatcctggtt
     3661 ctcgcgcaat acgtcgcgca cagcacttaa tgcattaaag taatttaatg gttgagtatc
     3721 tgtacttaat ttttcatgca ttttttgtgc attttgctgc ttgtggatat ttaaaatatc
     3781 gcgccatacc agtggagtcg taaatgtgtt ttgtttcagt tctgccagca taccttgcat
     3841 actggatgca atatcaccaa cgactggcac agcaatgggg cggttgctgt caatttcctg
     3901 cggttcaata tccagttgaa taaactgtgt atctgccgcc catccttttt taccgtgtgc
     3961 caataaccaa ttcagtcgtg caccaacaag catgacaacg tcagcatttg ccagggcaaa
     4021 cgaacgcgca gctgccgcag aaagtggatg cgtatcttca aggatccctt tcgccataga
     4081 cattggcagg aatggaatct gagtactttc aataaattca cgaagctgtt catcagcttg
     4141 tgaatacgcc gcgcctttgc caaggataat taatggccgt tcagcttttg ctaaaagcga
     4201 aattgcgcta gtgactgact tcgggcatgg caataatgct ggcgacggat tttcaacttt
     4261 aacaatcgtg gttaacgctt cgtctttttc catcgtcgcg gccaggacat ttgctggcaa
     4321 atcaagataa actccgccag ggcgacccga tacagagacc cggatagcgc gtgccaatgc
     4381 aatgccaaga tcctgcggct gattaacgcg aaatgctgct ttggcatacg gttttgccgc
     4441 attcatttgg tccagctctt cataatcacc ttgctgtagg tcgacgatcg cgcggtcgct
     4501 ggagccgcta atcatgatca tcggaaaacc atttaccgtt gcgttggcca atgcggtcaa
     4561 accattgagg aatcctggcg cagaaactgt caggcagatc cccggttttt gggtaagaaa
     4621 accgcttgcc gcagcggcat agcctgccga ctgctcatga cgaaaaccaa tataacgaat
     4681 gccttccgcc tgggcatggc gtgccatacc cgtcacagga atacctacaa caccataaat
     4741 agtgtcaata ttattctgtt ttaatgcttc aacgatgata tgcataccat ctgtcatttg
     4801 aagttgatct gacatgatta ataacctcga aatttttata aattgggagc cagaagcccc
     4861 cgttaatatc agatggcgtg gttttgcttc attgcagcaa tttcatcgtc actataaccc
     4921 agctcctgca atacagcagc ggtatgttca cctaatagcg gcgcagcttt aatatccggc
     4981 gtaaaggcag agaatttcat tggacaacca acggtcagat attttccacg caacggttgt
     5041 tccacttcaa caacactgcc actttggcgc aaagagggat caagtgaaat ttctttcata
     5101 cttaaaaccg gtgcacaagg aatatcaaac tgagtcaaat aggccaccgc ttcatgttta
     5161 tcaatagtga cagtgtattt ttcgatttca gcaaaaatat cgaaaatatg tggctgtcgt
     5221 gcatgggctg tactgtatgc cggatcggta atccattctg gtttgccgat ggctttacag
     5281 gtgttttccc agttttgctc ctgaatagtg aaataaatat aggcgttagg atcggtttcc
     5341 cagcctttac atttcaggat ccagccaggc tgaccgccac cacctgcatt accaccgcgg
     5401 ggaactgcat caccaaatgt accattcgga tactgcgggt attcttccag ataacccaat
     5461 ttatcgagac gctgctggtc acgtaatttc acgcggcaaa ggttcaatac ggcatcctgc
     5521 attgacatgg tgactcgttg cccacgcccc gttttttcgc gatgcagcaa agcagcaagt
     5581 aaaccgatca gcaaatgcat tccggtgttg ctgtcaccca acgctgcagc gcttaccagc
     5641 ggcggaccat cccaaaaacc cgtagtggat gccgcgccac ccgctgcctg agcaacgttt
     5701 tcataggctt ttacattcac ataaggcgaa cactcatcaa accctttgat cgaaccaaaa
     5761 atcagacgtg gattgatttc ttgaatatgc tcccaggtga agcccatgtg atcaatggcc
     5821 cctggatgaa agttctcgac taagatatca gcttcgcgga tcagcttttc cattacctct
     5881 ttgccttccg ctgttttggt atttaactca atagaacgtt tgttactgtt aagcatggtg
     5941 aagtaaagcg catcgatatc aggaatatct cgcagctggt gacgcgttac gtcaccaacg
     6001 ccgggacgtt caattttaat aacgtcagcg ccaaaccagg ccagcatttg agtacaagat
     6061 gggccagatt gcacaccggt gaaatcgaga actttaattc cttgaagtgg agttgacata
     6121 gcaataccta ttagttaatt aaatatgtgt taatttagcg tttatttaag gcgtaatatc
     6181 ttctataaat aacccgcaaa atatagtcgt agctgcactc gatattttta aaatcctgta
     6241 tcaagtgctg aaaatcattt catcgccact tccaattcct ttctgcatgg taagtagtga
     6301 ggacgaaaag aagaatatga taaatattga ttcgtggtaa tcagagagag tcgcgacatt
     6361 tgtgatgtta ttcttacaac tgtaagaagc gccggtgatt tacaagtaag ggtatgatta
     6421 cgctttttaa agcgtaccat aaaacataac ataataaatg atttgtgatg acacgggatt
     6481 attgggattt actttgagga agataaaatg cgtgtttata gaaccgcgag cgccaaataa
     6541 ttttatgacc acaacctgtc gtattgtggt cataaaaaat tgtcggctgg agggagttaa
     6601 atcctttacc tcaatatact gcctgtcagc attagttcac aaacagtgct tcgctgtcga
     6661 cgatgattcc atcttccgca cctttaagcg ccaggtttgc ctcgtagtat tgctttttat
     6721 caagcagttt ctgcgcatca gcaagtgcat tacgagtctg tttaagcggc atcagatatt
     6781 ggttttccat tacgccaaca ccggccaggc gaagttcttc cattgcgccc tttttatccc
     6841 ctttcgccat tttttcattg gcaattttta tcgcggcttc tttttcggga gttgctacat
     6901 aactctcaga aattccgaca gatgcattaa tgacaatata ttggtcatca ttgagattgg
     6961 tctttttacc tggcttagcg aactttgccc attcggtact gtcatctgac agcaaagcgg
     7021 aagcttcatt ggtcagttct tttgcctttt ctggatcgcc atgaaacaga gccaggcgcg
     7081 ctacctgcac gtcacgcatc gcatataaac cttgttcaga gatacgctct gctgctgccg
     7141 ttttctgggt ttgctgaact tgctgagttt gctgttgtgc cgctaccggt gcattatcag
     7201 cagcccatac agttgaagat gccagaattg ctgtgaccat cgtagccata attaaacgtt
     7261 tcatacttac ctcattcttt aacagtttta gaaaacgccg ccatcatatt gggtgagcat
     7321 gaaatgagaa atcagagagc aacggtcatt ttgaaagggt tatcttacag ttgtagttat
     7381 tatcgcaaga agtattttag tcttacaatt gtaggaatct gcttcctgtc gcagagaact
     7441 attcttatgt accacaaaga gatgtcattt attatatcga tgtaaatatt gattaattat
     7501 atgtaaatac agccataaca tcgtaggttc tcagtgaacc attttcagga taactttcta
     7561 tgaaagtaaa cttaatactt ttcagcttat ttttattggt ctctattatg gcatgcaatg
     7621 tttttgcatt ttccatttct ggtggtgtaa gtgagaggag ctataaagag actgaaaaaa
     7681 catcagcgat gacgaccaca cactctacaa aacttcagcc atcacaggcg attttgttta
     7741 agatgagaga agatgcgcca ccattaaacc tcacagaaga aatcacgcct acttatccga
     7801 caaaggcgaa ttatcttatt catcctgtgc gatagcgtct gtcacatggc aggtgaacaa
     7861 tcatgtctga tgctgatgtg aacacctgcc caaatgagcc atcctgaccc actatcgcct
     7921 gccatacccc accataaagc gaatcatcaa gccacaagcc ggggcaagaa acataaatat
     7981 ccccgtcact agttcattac cgccagttaa ccacatggtg aagtaataaa tgagtgcagc
     8041 gaggatcacc atgccgaaga aagaagagac cgccgatacc acgttcagta cctgaccgat
     8101 gtagccgctt acgcagacaa tacacagggc gagaaacatc cataaataaa tcatagctca
     8161 acctcctgcc gcgctaaaaa acctgtaact tcagtcactt taatagtaag acaatcctta
     8221 acctctggca atttttgcta agcatcaatg aaaacagcat gttaaatgca agactgttgt
     8281 gtacggaaaa atatttactt tgcacgatta ataatcagta gctgaaagca gtcagcgagt
     8341 gataaagtac agataccgca ttcatgctcc tcgtggttat gtcctgacta gtctttacac
     8401 tctttacagg aaccattgtc gtacatgatg gcccaaccaa ttgagtgtac cgctgtcgac
     8461 atggaatctt ctgcgataca acaattcgta tctacagaag gtaactatgt ttccacaatg
     8521 caaattttcc cgcgagtttc tacatcctcg ctactggctc acatggtttg ggcttggtgt
     8581 actctggctt tgggtacagc ttccttatcc tgttctctgc tttctcggca cgcgtattgg
     8641 cgcaatggcg cgaccattcc tgaaacgtcg tgaatctatc gcccgtaaaa acctggaact
     8701 ttgtttcccg cagcattctg cggaagaacg cgagaagatg attgccgaaa actttcgttc
     8761 actcggcatg gcgctggtag aaaccggcat ggcatggttc tggcccgaca gtcgcgtacg
     8821 taaatggttt gatgttgaag ggttggataa ccttaaacgc gcacaaatgc aaaatcgcgg
     8881 cgtaatggtt gtcggcgtcc attttatgtc gctggaactg ggcggccgcg tgatgggact
     8941 gtgccaacca atgatggcta cctatcgtcc acataataat cagctgatgg aatgggtgca
     9001 gacccgtggg cgcatgcgct ctaacaaagc gatgatcggc agaaataatc tgcgcggcat
     9061 tgtcggtgca ctgaagaaag gtgaagcggt atggtttgct cccgatcagg attatggtcg
     9121 taaaggcagc tccttcgcgc cgttctttgc ggtggaaaat gtcgccacaa ccaatggcac
     9181 ctatgttctc tcccgtctct ctggcgcagc catgttgacc gtaacgatgg taagaaaagc
     9241 ggattacagc ggatatcgtt tgttcatcac cccagagatg gaaggctacc cgacagatga
     9301 aaatcaagcc gctgcctata tgaacaagat tatcgagaaa gagatcatgc gcgcaccgga
     9361 gcagtacctc tggatccacc gtcgctttaa aacgcgcccg gtgggagaat cgtcgttgta
     9421 catttaacaa actgctctta ctctttccct cctcaaatcg gccataataa cctcatggtt
     9481 tttatggctt ttttcttaag gttttttcct tcgctgaata tctaattatt accgtcagaa
     9541 attaaactgt cgccgatatg agacacttgt aagttactaa aagtaaataa gaaaatgtct
     9601 cttgtctcgt ttcagtttta ggcgtacatt agcgccgtct cgagcagttt agctcagaat
     9661 tctggggtga tgcccacggt aaaatacagg gcttttaagg tcgctttgcc cggtgtcaac
     9721 tcactgtatc aggtgtaatg aagtcattca ggcgtaacag taattacgcg gagagatgta
     9781 aagtgaaata tttctttatg ggcatttcat ttatggtcat cgtttgggcc ggtacttttg
     9841 ccctgatgat ctaaaagcaa actgtgcaat gaaaaacagg agccatcagg ctcctgtttg
     9901 catttctgac gacggtgttt attccgcgtt ttcgtgaata tgtttgctgc tggcgggtag
     9961 taaaccgtcg gcacggaaca tcgctttaat cccacgaatc gcctgacgaa tacggtcgcg
    10021 gttttcaatc agtgcaaagc gaacatgcgt atcaccatag tcgccaaagc caatccccgg
    10081 cgagacacaa accttcgctt cgttaaggag cttcttggca aattccagcg atcccatggc
    10141 cgcatatggt tccgggattt tcgcccagac atacatcgaa gccttcggca tttcaaccat
    10201 ccagcccgct tcatgcagcc ctttaaccaa tacatcacgg cggcgtttgt actgttcggc
    10261 aatgtcgcgc acgcactgtt gatcgccctc cagcgccgca atcgccgcca cctgcaacgg
    10321 cgtaaaggtg ccgtaatcgt gatagctttt aatacgagcc agggcgctga ccagcgtttt
    10381 gttgccaacc ataaagccga tacgccagcc cgccatgttg tagcttttcg acagcgtaaa
    10441 gaactcgact gccacatcgc gtgcacccgg tacctgcatg attgacggcg ctttccagcc
    10501 atcgtagacg atatcggcat aggccaggtc gtggaccacc agcacatcgt agcgtttcgc
    10561 cagcgccaca accttttcga agaactccag ctccacgcat tgcgcggttg gattcgacgg
    10621 gaagccgagg atcatcatct tcggtttcgg ataactttca cgaatggcgc gctccagttc
    10681 gttgaagaaa tcgacacctt ccaccagcgg cactgagcgt acctgcgccc cggcaatcac
    10741 cgcgccataa atatggatcg ggtaacttgg attcggcacc agcaccgtgt caccatgatc
    10801 cagcgtcgcc agcatcagat gcgccaggcc ctctttcgaa ccaatagtga cgatggcttc
    10861 tgattccggg tcgatttcaa cgtcgtagcg atcctgatac cagcgggaaa tggcgcgacg
    10921 taaccgcgga atgccgcgtg aagtggagta accatgcgtg tccgggcgct gggccacagt
    10981 acataatttt tcgacgatat gcggcggagt cgcaccgtcc gggttaccca tgctgaaatc
    11041 gataatatct tcgccgcgcc gacgcgcagc cattttcagt tcagcggtga tgttaaaaac
    11101 gtagggcggg agacgatcaa tgcgcgtaaa gcgacgttca gggcgagtgt cagccataat
    11161 ttcctcagat taacgttagc gcccggaccg tccgagcgac gctgccacaa tggtggctgt
    11221 tttgaaaata gcctgattaa tttctgtctg tccagcgttt tagaacattt ttatcaatgg
    11281 ctttaaatga ggaactccac ttcaaaaaca ggaaattttt ctacgctaac aagaaaatta
    11341 ccaccaattt tattaacatt gtaatttc
//
LOCUS       DAJOWB010000133        11186 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJOWB010000133 DAJOWB010000000
VERSION     DAJOWB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.133"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            94..198
                     /locus_tag="OHO85_003981"
     CDS             94..198
                     /locus_tag="OHO85_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCQ2673286.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            509..2707
                     /gene="speF"
                     /locus_tag="OHO85_003982"
     CDS             509..2707
                     /gene="speF"
                     /locus_tag="OHO85_003982"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCQ2673287.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKETRPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDVAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2704..4023
                     /gene="potE"
                     /locus_tag="OHO85_003983"
     CDS             2704..4023
                     /gene="potE"
                     /locus_tag="OHO85_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCQ2673288.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4079..5719)
                     /gene="pgm"
                     /locus_tag="OHO85_003984"
     CDS             complement(4079..5719)
                     /gene="pgm"
                     /locus_tag="OHO85_003984"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCQ2673289.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5745..6290)
                     /gene="seqA"
                     /locus_tag="OHO85_003985"
     CDS             complement(5745..6290)
                     /gene="seqA"
                     /locus_tag="OHO85_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCQ2673290.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6475..7239
                     /gene="ybfF"
                     /locus_tag="OHO85_003986"
     CDS             6475..7239
                     /gene="ybfF"
                     /locus_tag="OHO85_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCQ2673291.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDPPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7379..7672
                     /gene="ybfE"
                     /locus_tag="OHO85_003987"
     CDS             7379..7672
                     /gene="ybfE"
                     /locus_tag="OHO85_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCQ2673292.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7812..8342
                     /gene="fldA"
                     /locus_tag="OHO85_003988"
     CDS             7812..8342
                     /gene="fldA"
                     /locus_tag="OHO85_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCQ2673293.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLYEILNA"
     gene            8552..8638
                     /gene="uof"
                     /locus_tag="OHO85_003989"
     CDS             8552..8638
                     /gene="uof"
                     /locus_tag="OHO85_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCQ2673294.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8631..9077
                     /gene="fur"
                     /locus_tag="OHO85_003990"
     CDS             8631..9077
                     /gene="fur"
                     /locus_tag="OHO85_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCQ2673295.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9162..9488)
                     /locus_tag="OHO85_003991"
     CDS             complement(9162..9488)
                     /locus_tag="OHO85_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673296.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9538..10944)
                     /gene="chiP"
                     /locus_tag="OHO85_003992"
     CDS             complement(9538..10944)
                     /gene="chiP"
                     /locus_tag="OHO85_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCQ2673297.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2706 a         2758 c         2790 g         2932 t
ORIGIN      
        1 tgatcgtttt ttgttgatct tcttcacaga ttatagccat ttcatggata gaataactct
       61 accttcaact gacacagcaa gaggtaaagg taaatggaaa ataataaccg cttaatgcct
      121 catataaggc ggacaaccca tatcatgaag tttgcccatc gtaatagttt tgactttcat
      181 ttctttaatg cccgctagtc ttctgactaa agggcacccc aacgtacagg tctccctgac
      241 tttaagcatt acaggttaat acctgtattc ctcggtgctc atatactgct aaccctttta
      301 aactctaaat aattcgagtc gcagcacttg caacttgagg tatgacgagt atagccagtt
      361 accgggctgg tctgggttat tgcatctgca aaaagcaaac tactgattta tttatcagcg
      421 gtggagcttt gctttttttc cggcgtgatc gatttctcct ttgagaaatt gaggacctgc
      481 tattacctga aataaagaga tgaacaaaat gtcagaatta aaaattgccg ttagtcgttc
      541 ttgcccggat tgtttttcca ctcatcgtgc atgcgtgaat atagacgaaa gtaattatat
      601 tgacgttgcc gccattattt tatcagtcag tgatgttgaa cgtggaaaac tcgatgaaat
      661 agacgctact ggctatgaca ttcctgtttt tattgcaacg gaaaatgaag aacgtgtacc
      721 cgcagaatat ctgccacgta tttctggtgt cttcgaacat tgcgaatcac gtaaagagtt
      781 ttatggacgt cagttagaaa ccgctgccag ccattatgaa acgcaactgc gcccgccctt
      841 cttccgcgca ctggtcgatt atgttaatca gggcaatagt gcttttgact gccctggtca
      901 tcaggggggt gagtttttcc gtcgccatcc ggcaggcaat cagttcgtgg aatactttgg
      961 cgaaatgctc ttccgctctg acttgtgtaa tgctgacgta gcaatgggcg atctgctgat
     1021 tcatgaaggt gcgccatgta ttgcacaaca gcatgcggct aaagtcttta atgccgataa
     1081 aacctatttc gttttaaacg gtacttcatc ttctaataaa gtggtcttaa acgcattgct
     1141 gacgccgggc gatctggtgt tgtttgaccg caataaccat aaatctaacc accacggtgc
     1201 gttgctacag gctggcgcga caccggttta tctggaaacc gcacgtaacc cgtatggctt
     1261 tatcggtggc attgatgcgc actgttttga agaaagttac ctgcgtgagc tgattacaga
     1321 agttgcaccg cagcgggcaa aagagactcg tcctttccgc ctcgccgtta ttcagctagg
     1381 cacttacgac ggtacgattt ataacgcccg tcaggtggtg gataaaattg gtcatctgtg
     1441 tgactacatc ctctttgact ccgcatgggt cggctatgaa cagtttattc cgatgatggc
     1501 tgactgttcg ccgctgttgc tggatcttaa tgagaacgat ccgggtattc tggttacgca
     1561 atccgtacat aaacagcagg ctggtttttc tcaaacttca caaatccata aaaaagacag
     1621 ccacatcaaa gggcaacagc gttatgtacc gcacaaacgc atgaacaacg cctttatgat
     1681 gcacgcctcc accagcccgt tctatccgct gtttgccgca ctggatatca acgccaaaat
     1741 gcatgaaggt gtcagcggtc gtaatatgtg gatggattgt gtggtaaatg gcattaatgc
     1801 ccgcaaactg atcctcgata actgtcagca tattcgtccg ttcgtacctg aactggtgga
     1861 tggtaaaccc tggcagtcgt atgaaacagc gcaaattgcg gttgatctgc gcttcttcca
     1921 gtttgtacca ggggaacact ggcattcttt tgaaggctat gcagagaatc aatactttgt
     1981 cgatccttgc aaactgttgc tgacaacccc aggtattgat gcacgtaacg gcgaatatga
     2041 agcgttcggt gtacccgcga cgattcttgc taacttcctg cgcgaaaatg gcgtagtgcc
     2101 ggaaaaatgc gatcttaact ccatcctctt cctgctgact ccggcagaag atgtggccaa
     2161 gcttcagcaa cttgttgccc tgctggtacg cttcgaaaaa ctgcttgagt ccgacgcgcc
     2221 attagcagaa gtgctgcctt ccatctacaa acagcatgaa gagcgctacg ccggttatac
     2281 cctgcgtcag ttgtgtcaag aaatgcatga tttgtatgcc cgtcacaacg tgaaacaact
     2341 gcaaaaagag atgttccgta aggaacactt cccacgcgta agcatgaatc cgcaagaagc
     2401 caactacgcc tatttacgcg gtgaagtgga gctggttcgt ctgccggatg cagaaggccg
     2461 tatcgctgcc gaaggtgcgc ttccttatcc tccgggtgtg ctgtgcgttg ttccgggtga
     2521 aatctggggt ggtgctgttc tgcgttactt cagcgctctg gaagaaggga tcaacctgtt
     2581 gccaggtttt gcaccggagc ttcagggtgt ctatatcgaa gaacatgatg gtcgtaagca
     2641 agtttggtgc tatgtcatca agcctcgtga tgcgcaaagc accctgttga aaggggaaaa
     2701 attatgagtc aggctaaatc gaacaaaatg ggcgtcgttc agttaaccat actgacgatg
     2761 gtcaacatga tgggctccgg tatcatcatg ctgccgacaa agcttgccga agttgggaca
     2821 atttcaatta tctcctggct ggtgacagcc gtaggctcaa tggcactggc atgggcattc
     2881 gcaaaatgcg gtatgttcag ccgtaaatca ggtggtatgg gcggttatgc cgagtatgct
     2941 ttcggtaagt ccggcaattt tatggcgaac tacacctacg gtgtctcgct gctgattgct
     3001 aacgtcgcga ttgctatttc ggcggttggt tacggcaccg aactgctcgg cgcgagtttg
     3061 tcgccagtgc agattggtct tgcgaccatc ggagtgctgt ggatttgtac cgtggctaac
     3121 tttggtggtg cgcgcattac cgggcaaatc agtagcatta ctgtgtgggg ggtcattatt
     3181 ccggtggtag gcctgtgcat tatcggctgg ttctggttta gcccgacgct ttacgttgat
     3241 tcctggaatc cgcatcatgc accgttcttc agtgcagtag gttcttccat cgctatgacg
     3301 ctgtgggctt ttctcggtct ggagtctgcg tgtgcgaata ctgatgtagt ggaaaacccg
     3361 gaacgtaatg tgccaatcgc ggtactcggc ggtacgttag gtgcggcggt gatttatatc
     3421 gtctccacca acgtgattgc agggattgtg cctaatatgg agctggcaaa ttcaacggca
     3481 ccatttggcc tggccttcgc acagatgttc acaccagaag tgggtaaagt cattatggcg
     3541 ctgatggtga tgtcctgctg cggttcgctg cttggctggc agttcaccat tgcccaggtg
     3601 tttaaatctt catctgatga aggctacttc cctaaaattt tctcccgtgt aaccaaagtg
     3661 gatgcaccgg tgcagggaat gttgaccatt gtgattatcc agagcgggtt ggcgctgatg
     3721 acgattagcc cgtcgctaaa cagtcagttc aacgtgctgg ttaacctggc cgtggtgacc
     3781 aatatcattc cgtatattct gtcaatggcg gcattagtca ttattcagaa ggtcgctaat
     3841 gtgcccccct caaaagcgaa agttgcaaac tttgttgctt ttgttggcgc gatgtatagc
     3901 ttttatgcgc tgtactcatc cggggaagaa gccatgctgt acggttccat cgtaaccttc
     3961 cttggttgga cactgtatgg tctggtatca ccacgctttg aactgaaaaa taaacacggt
     4021 taaaaaagca aagtaaaaaa gggcgatctt gcgaccgccc tttttttatt aaatgtgttt
     4081 acgcgttttt cagaacttcg ctaacaatct caaccgcttc tttctcaatc tgcttgcgat
     4141 gttcttcacc gaggaagctt tcgcagtaga tcttatatgc gtcttccgtg cctgacggac
     4201 gcgcggcgaa ccagccgttg tcagtcatca ctttcagacc gccgatagaa gcaccattgc
     4261 ccggtgccgc cgtcaggcgc gcggtgatcg ggtcgcctgc cagggtgctg gcgctcacca
     4321 tttccggaga cagcttagac agcgccgctt tttgtgcgga agtcgcagct gcctgcaaac
     4381 ggttgtagct cggcgcacca aagcgttttg ccagttcgtt gtagtgttcc tgcgggttct
     4441 taccggtgac cgcggtgatt tccgccgcca gcagacacat gatgatgccg tctttgtcgg
     4501 tggaccacgg cgtgccgtcg aaacgcagga aggaagcccc tgcgctctct tcgccgccaa
     4561 aaccgaagct gccgtcgaac agaccatcaa caaaccattt gaaacctacc ggtacttcta
     4621 ccagcttacg gcccaagtca ttgaccacac ggtcgatcat cgcagaagaa accagcgttt
     4681 taccgacagc aacatctttg ccccactgcg gacgatgctg gaacaggtag ttgatcgcca
     4741 ccgccaggta gtggttcgga ttcatcaaac ctgccggagt gacgataccg tgacggtcat
     4801 aatccgggtc gttagcaaac gccagatcga acttatcacg cagtgccagc aggcccgcca
     4861 tcgcacactc ggaggagcag tccatacgga tcgcgccgtc tttatcaagg tgcataaagc
     4921 ggaaggtttg atcgacctga tcgttaacga tggtcaggtt gaggttgtaa tactcgccaa
     4981 tacgcttcca gtattcgata ccggaaccgc ccagcggatc aacgcccagc gtcaggcccg
     5041 ctttctgaat tgccgccata tcaacgatat cggccagacc ttccacaaac ggctgcacca
     5101 gatcctgctc ttttacatga ccggatgcca tcgcttcgtc gagggagata cgcttcacgc
     5161 ctttcaggcc atcggccatc agtgcgttgg ccctgtcttc caccactttg gtgacgttgg
     5221 tatcagccgg gccaccattt ggcgggttgt acttgatacc accatcttcc ggcgggttat
     5281 gggacggtgt aatcacgata ccgtctgcca gcgggccacc ttttttattg tgaaccagga
     5341 tggcattgga aatggcaggt gtcggagtga agccattgtt ttcctgcaca atgacatcaa
     5401 cgccgttcgc tgccagcact tccagtacgg aaatgaacgc aggctcggac agggcgtgag
     5461 tatctttacc cacatagcaa gggccagtga tgccgttttt cgcacgttct tcagcaattg
     5521 cctgagcgat cgccagaatg tgcggctcgt taaagctgtg gcgcgctgca ctgccacggt
     5581 gaccggaagt accgaatttc accgcgtgct ccgcattccc tgcttctggt ttcagtacat
     5641 aatattgcgc cgtcagttgg gcgacgttaa tcaaatcact ctgttgtgca ggttggcctg
     5701 cacgattgtg gattgccatt gctttgtcct ttgtctgcaa cgttttagat agttccgcaa
     5761 accttctcaa tcaattccgc cgggaattgc atcgactgca tgatgtgttc gatcatgctg
     5821 catttacggc cggtgttggt gttggtgatc acccaatacg gcgtgcctgg cacatgtttc
     5881 ggcttggtct gattaccatt tttcagcagc gtttgttcat ctgccgcaaa gtaaacgcgt
     5941 gtacgaccgt gcaacgattc cgttgcttcg gcaaacgcct gggcgtcaag agaatatagt
     6001 gtagacaaca gcagcataaa gcgattgacc gctcgctttt gctctgcgta ttcgtccgaa
     6061 agcagaagtt cacgcattgc gcgaaccttg tctttaatcg ttttgaccgg cttcgcttcg
     6121 acgatagcag gtgacgcaac gcgaacctct ttcgtcaccg gagcagcagg ctgtgatgcg
     6181 gcggaaaatt tcaacatacg ccgtaaaatg tcggatgcgc tctcgccgat atgcttagtg
     6241 tggctggcaa tatagctgta gagttcatca tcaacttcaa tcgttttcat cttaatccag
     6301 tgcagtgtct tagctgaata caagtcgcca ggagtatatg aataaatccc ggcagcggat
     6361 agcgtcaagc ctggcttgca gaaaaactcg gaataaacct tatttgttgc agccctgtgg
     6421 cacaatggtc aacataatac cctaacccga caacgcgaaa aaaagaactt tgccatgaaa
     6481 ttgaatatcc gcgcgcaaac tgcacaaaac cagcacaata attctcccat cgttcttgtc
     6541 catggtctgt ttggtagcct cgacaacctt ggcgtactgg ctcgcgatct ggtaaacgat
     6601 cacaatatca tccaggttga tatgcgtaac cacggtcttt caccgagaga gccggtaatg
     6661 aattacccgg cgatggcgca ggatcttgtt gataccctgg atgcactgca gatcgacaaa
     6721 gcaacattta tcggtcactc catgggcggt aaagcggtaa tggcacttac tgcactggcc
     6781 cccgatcgca tcgataaact ggtggcgatc gatatcgcac cggtcgacta tcacgtacgc
     6841 cgtcatgatg agattttcgc agctatcaac gcggtcagtg aatcggacgc acaaactcgc
     6901 cagcaagcag cagcaataat gcgccagcat cttaatgaag aaggggtgat tcagtttctg
     6961 ttgaaatctt ttgttgacgg ggagtggcgc tttaacgtgc cggtattgtg ggatcagtat
     7021 ccgcatattg tgggttggga gaaaatcccg gcatgggatc cccctgccct gtttatccct
     7081 ggcggcaatt ctccgtatgt tagcgagcag taccgtgatg atttactggc acaatttcca
     7141 caggcacggg cgcatgtgat tgcaggcgcg ggtcactggg tccatgctga aaaaccggat
     7201 gcggtattac gcgctatccg tcgctatctc aatgattaac gcaactgatt aaaaacccag
     7261 cgtccgcgtc gctttccgcg cgtgggcgtt ggcgtgactc atcgcgctga tgtattatgg
     7321 cgcgctatcc atccgggcag aggcctggct gattgtttcc cccgaagtca ccaagatcat
     7381 ggccaaagaa caaacggacc gtacgacatt agatctgttc gcgcacgagc gtcgaccggg
     7441 acgaccgaaa actaatccgc tttcgcgcga tgaacagctg cgtattaata aacgcaacca
     7501 gctaaaacgc gacaaagtac gtggccttaa gcgtgtcgaa ctgaagctga acgcggaagc
     7561 tgtcgaggcg ctgaacgagc tggcggagtc gcgcaatatg agccgtagcg aactgatcga
     7621 agagatgtta atgcagcaac tggcggcgct gcgtagtcag ggcatcgttt aaatttccac
     7681 tttcatgtag cacagtgtgc agtcctgctc gtttgctgat tccgcaagac tgcctgttct
     7741 gctatgattg cctttatccg tgggcaattt tccaccccca tttcaataag tttcaagagg
     7801 ttatttcact catggctatc actggcatct ttttcggcag cgacaccggt aataccgaaa
     7861 atatcgcaaa aatgattcaa aaacagcttg gtaaagacgt tgccgatgtc catgacattg
     7921 caaaaagcag caaagaagat ctggaagctt atgacattct gctgctgggc atcccaacct
     7981 ggtattatgg cgaagcgcag tgtgactggg atgacttctt cccgactctc gaagagattg
     8041 atttcaatgg caaactggtt gcgctgtttg gttgtggtga ccaggaagat tacgccgaat
     8101 atttctgcga tgcattgggc accatccgcg acatcattga accgcgcggt gcaacgatcg
     8161 ttggtcactg gccaactgcg ggctatcatt tcgaagcatc aaaaggtctg gctgatgacg
     8221 accactttgt cggtctggct atcgacgaag accgtcagcc ggaactgacc gctgaacgtg
     8281 tagaaaaatg ggttaaacag atttctgaag agttgcatct ctacgaaatt ctcaatgcct
     8341 gatgtgatgc ggcgtagact catgtctacg ccgtattaat agataatgcc aatcaaaata
     8401 attgctacaa atttgtaact tttgctgttg tacctgtaca atgtcccggt gttcaagtgg
     8461 ccttgccgtt gtaaatgtaa gctgtgccac gtttttatta acaatatttg ccagggactt
     8521 gtggttttca tttaggcgtg gcaattctat aatgatacgc attatctcaa gagcaaattc
     8581 tgtcacttct tctaatgaag tgaaccgctt agtaacagga cagattccgc atgactgata
     8641 acaataccgc cctaaagaaa gctggcctga aagtaacgct tcctcgttta aaaatcctgg
     8701 aagttcttca ggagccggac aaccatcacg tcagtgcgga agatttatac aaacgtctga
     8761 tcgatatggg tgaagaaatt ggtctggcta cggtatatcg cgtactgaac cagtttgacg
     8821 acgctggtat cgtcacccgc cacaattttg aaggcggtaa atccgtattt gaactgacac
     8881 agcaacatca ccacgatcac ctgatctgcc tcgactgcgg caaggttatc gaatttagtg
     8941 atgattccat cgaagcgcgt cagcgtgaaa ttgccgcaaa acatggcatt cgcctgacta
     9001 accacagtct ctatctttac ggtcactgtg ccgaaggcga ttgccgcgaa gatgaacatg
     9061 cgcacgaagg caaataagcc agcctgaatg agaaaaagcc aacctgcggg ttggcttttt
     9121 tatgcaaggg aaataagaaa agcgagagtt acagctctca cttatttgtt attactgcga
     9181 atttcctgcc aaactttatc acaatcggct ttcaccgcct gatcatttcc ggtttgcgtt
     9241 gcacgcagac actgctgata atccagtacg cggacacttt cctggtcggc aaaatgctga
     9301 tgttgtttct ctttcttcag cacgtttaac acgctctggc aggcttcaat tttttctggc
     9361 gaaccttgtg cggtgttgat acaggcgctg tatgcctctt tcagacggct gtcttcctgt
     9421 ggcgcggttg attgcgcaca agccaccagc cccgatgcca tcatggcgat gagaatcagt
     9481 tttttcatag caaattcctc aaatgtgcgg caggcacaaa acctgccgcg tcgggcatca
     9541 gaagatggtg aatggtgcga ttaccataaa tttcacgtca cgctcatcct ggaagatgtt
     9601 gccgtaacca ccgccccagc ttgggatgtc ggagtggttg tcgtattcgg tgaaatgcag
     9661 tttgaacatc gtgcctttgg cgcgaccgtc ctgaatggtg tagaccgcat ccaggctgta
     9721 tgcagactct tcaatagtcc ggtttttgtc gtagtacgca tccggattgc tctgccaggt
     9781 cgcaggttta gcatcccatg cgtaaacgta ggaagcgccg atggcgaagc ctggaagatt
     9841 ccagtttttc aggtcataca tcgcaccgaa gaagactgct ttttcgccgt tggcgttgaa
     9901 gtcagaacgg ttgtcccacc agatatccag gcgaccgttt gaggaagcgt aggttggagt
     9961 catacgttgc aggaagtatc cctgctgacc gtcagcctta acccaggtgc cttcgaggcg
    10021 caaatccact acgtcagccg cccggtaacc aaaggtcaac gcctgcagcc aggcggtgcc
    10081 gtcataaagg tcgttgacgc tgcgatcgtc aactttatcg cgggtaccgt agaactggta
    10141 gctggtggtt aacgggctac cggcgatatc aaatttgtag ctggctttgg caaaatattg
    10201 atcgatatac ccttccgcct gaccaaaagc cgcttccagt acgaagttat ttttgaagtc
    10261 gtatttcgcc ccaagggagt gcagataatc aactttggtg gttttatcgt tctgataaaa
    10321 ctcatccatt tccagatgcc acggcgcttt gtattcgttg gtccacatgt aggagaaact
    10381 caacgcaccg gcatcgccgt aatcaaaatt cgctccggct tccgcaccct gataagtacc
    10441 cggcataaag ctccagtgag gcgctaacag cgtctgaccg gttggctgaa tataacctgc
    10501 cctcgcccaa accggaccgt atttaaattt ggccgctgct ttatacaggc ttataccgct
    10561 tttgtcgccg gaccagtctt cgtcataggc tttattactt tttgaaaacg cgatttcgtt
    10621 cgggtgagag ctgtcgccgt tttccgccat ttcaatcgcc gtaaacgcgg caatatcaag
    10681 gccgaacata tcagcagcat aaccagactg aaaatcgagg ttggcattcc aggtggagtg
    10741 agaaaggttg gttttgtatt tgtcgccgtc ggtaacatct ttacggtcgc gttcacgctg
    10801 ccagtaatag ataccgccgg ttaaggttga atcgtcgatg aatccttcgg cgcgagcctc
    10861 cggcattgcc ataaagcccg acattgctgt aacaccggcg atagccagcg ccagcgtact
    10921 acgtttgcca ctaaacgtac gcatgggtta atcctctttg acgtataaat tgctgcacca
    10981 aaggtgaaca gcgaaaaaaa ataaaaattt aaaaagcagc gttgagaaaa gtctcgctga
    11041 ctacaggaac agactatttt tcgcgacgcg aattatcaat ctctttctgt aaccagaatg
    11101 taagaacatg agcaaccgca catattttga tgatttttgt tacaaggctt aagtttgcgg
    11161 ttgaaaggct tgcagaagaa taaaag
//
LOCUS       DAJOWB010000134        11175 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJOWB010000134 DAJOWB010000000
VERSION     DAJOWB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.134"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            16..582
                     /locus_tag="OHO85_003993"
     CDS             16..582
                     /locus_tag="OHO85_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2673298.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            633..3092
                     /locus_tag="OHO85_003994"
     CDS             633..3092
                     /locus_tag="OHO85_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCQ2673299.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTTGINAGAWRLRSDYQLNQTDSDDNHEQSGGIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDERMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3107..3838
                     /locus_tag="OHO85_003995"
     CDS             3107..3838
                     /locus_tag="OHO85_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCQ2673300.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3835..4896
                     /locus_tag="OHO85_003996"
     CDS             3835..4896
                     /locus_tag="OHO85_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2673301.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5048..6094
                     /locus_tag="OHO85_003997"
     CDS             5048..6094
                     /locus_tag="OHO85_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCQ2673302.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6091..6882)
                     /gene="nei"
                     /locus_tag="OHO85_003998"
     CDS             complement(6091..6882)
                     /gene="nei"
                     /locus_tag="OHO85_003998"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCQ2673303.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6918..7652)
                     /gene="pxpA"
                     /locus_tag="OHO85_003999"
     CDS             complement(6918..7652)
                     /gene="pxpA"
                     /locus_tag="OHO85_003999"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCQ2673304.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMHACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEQQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7642..8574)
                     /gene="pxpC"
                     /locus_tag="OHO85_004000"
     CDS             complement(7642..8574)
                     /gene="pxpC"
                     /locus_tag="OHO85_004000"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCQ2673305.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8568..9224)
                     /gene="pxpB"
                     /locus_tag="OHO85_004001"
     CDS             complement(8568..9224)
                     /gene="pxpB"
                     /locus_tag="OHO85_004001"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCQ2673306.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9247..9990)
                     /gene="ybgI"
                     /locus_tag="OHO85_004002"
     CDS             complement(9247..9990)
                     /gene="ybgI"
                     /locus_tag="OHO85_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCQ2673307.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10261..>11175
                     /gene="dtpD"
                     /locus_tag="OHO85_004003"
     CDS             10261..>11175
                     /gene="dtpD"
                     /locus_tag="OHO85_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCQ2673308.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFG"
BASE COUNT         2699 a         3009 c         2726 g         2741 t
ORIGIN      
        1 ttaaaggact ccgttatgtt taaaggacaa aaaacattgg ccgcactggc cgtatctctg
       61 ctgttcacag cacctgttta tgcggctgat gaaggatccg gtgaaattca ctttaaaggt
      121 gaagttattg aagcaccgtg tgaaatacat caggatgata ttgataaaga ggttgaactc
      181 ggtcaggtga ccaccagcca cattaatcag tcacatcaca gcgatgccgt tgctgtcgac
      241 ctgcgcttag tcaactgtga tctggaaaac tccagcaacg gttccggtgg caagatttct
      301 aaagttgcag tcacctttga tagctcagcg aaaaccaccg gcgcagatcc gattctcaac
      361 aacaccagca cgggtgaagc caccggcgtc ggcgtacgtt taatgaataa agatcaaagc
      421 aatatcgttt taggcaccgc tactccagat atcgacctgg ctccgacctc cagcgaacaa
      481 acgctgaatt tctttgcctg gatggaacaa attgatcagg ctacacctgt aacgccaggc
      541 gcagttacgg cgaatgcaac ctacgtgctg gattataaat aagtattact ttggcggcgc
      601 tatcgccgcc tttcataaaa tatttatcag gtatggacac cgtgaatatt tatcgactct
      661 cttttgtatc ctgcctggtc gtggcgatgc cttgcgcatt ggcggtcgaa ttcaacctta
      721 atgttctcga taaatcaatg cgcgaccgca ttgatatttc attattaaag gaaaaaggag
      781 tcattgctcc cggtgagtat tttgttagcg ttgcggtaaa taataaccaa atcagtaacg
      841 ggcaaaagat taactggcac aaaaatgacg ataaaaccat tccgtgcatc aatgatttac
      901 tggtcgataa atttggctta aaacctgaag tccgtcagtc gttaccattg ataaatcagt
      961 gcgtcgattt tagctcccga cctgaaatgc tcttcaattt cgatcaagcc aatcagcaac
     1021 taaatatcac cattccgcaa gcctggctgg cgtggcactc agaaaactgg accccaccct
     1081 ccacatggaa agaaggtgtc gccggtgtcc tgatggatta caacttgttt gccagcagct
     1141 accgcccaca ggatggcagc agcagcacta acctgaacgc ctacggtacc accggaatta
     1201 acgccggggc atggcgctta cgtagtgatt atcagttgaa tcagactgat agcgatgata
     1261 accatgaaca gtcaggcgga atatcgcgca cctatctttt tcgtccatta ccgcaattag
     1321 gctctaaatt aaccctcggc gaaacggatt ttagttccaa tattttcgac ggtttttctt
     1381 ataccggcgc ggcactggca agtgacgagc gaatgttgcc atgggaacta cgcggctacg
     1441 ccccacaaat tagcggtatt gcacagacca atgccacggt gacgatcagt caatcaggcc
     1501 gcgtcattta ccagaaaaaa gtcccaccag gcccatttat cattgacgac cttaatcagt
     1561 ctgttcaggg cacactggat gtcaaagtga cggaagaaga tggtcgggtg aacaatttcc
     1621 aggtttcggc agcatcgacg cccttcctga ctcgtcaggg acaggttcgc tataaactgg
     1681 ccgcgggtca gccacggccc tccatgtcac atcaaactga aaatgaaacc ttttttagca
     1741 atgaagtttc ctgggggatg ctgtcaaaca cctcgctgta cggcggcctg ctgctttctg
     1801 gtgatgacta ccattctgcc gcaatgggta ttgggcaaaa tatgctgtgg cttggtgcgc
     1861 tgtcgtttga tgtcacgtgg gccagtagcc attttgatac tcagcaggac gagcggggct
     1921 taagctaccg ttttaattac agcaaacaag tggatgccac caacagcacg atttccctcg
     1981 ccgcttatcg tttctccgat cgtcattttc acagctacgc caactatctt gatcacaaat
     2041 acaacgacag cgatgcgcag gacgaaaaac agacgatcag cttatctgtg ggccaaccga
     2101 ttaccccact aaacctcaat ctttacgcca acctgctaca tcaaacctgg tggaatgcag
     2161 acgcctccac gaccgccaac atcacggcag gttttaatgt tgatattggt gactggagag
     2221 atatctctat ttcgacgtca ttcaatacaa cccattacga agataaagat cgcgacaacc
     2281 agatttacct gtcgatttcg ctccccttcg gtaacggtgg tcgggttggc tatgacatgc
     2341 aaaacagtag ccacagcacc acacaccgca tgtcgtggaa cgatacgctg gatgaacgta
     2401 atagctgggg catgtctgcc ggactgcaat ccgaccgtcc ggacaatgga gcccaggtga
     2461 gcggtaacta tcagcatctg agttcagcgg gtgagtggga tatttctggt acctatgccg
     2521 ccaatgatta cagttccgtc agcagcagct ggagcggttc tttcaccgca acccaatatg
     2581 gtgcagcgtt tcatcgccgc agctccacca atgaaccacg cctgatggtc agcaccgatg
     2641 gcgtggcaga tattccggtc cagggcaatc tcgactacac caaccatttt ggcattgcgg
     2701 tggtgccgtt gatttccagt taccagcctt ccaccgtggc ggtgaacatg aatgacttac
     2761 ccgacggcgt aacagttgca gaaaacgtta tcaaagaaac gtggattgaa ggcgcgatag
     2821 gttacaaatc actggcttcc cgttccggta aagacgttaa cgtcatcatt cgcaacgcca
     2881 gcggtcagtt tcctcccctc ggagcggata tccgccagga tgacagcggc attagcgtgg
     2941 ggatggttgg cgaggaagga catgcctggt taagcggagt cgctgaaaat caaaagttta
     3001 ccgtggtctg gggtgatagc cagcattgct cgctccatct tcctgaacat atggaagaca
     3061 ccgcaaatcg cctgatttta ccttgtcatt aattaaggaa atgaaaatga catttatgaa
     3121 aggactaccc cttttgttgt tggttgccag tctgtgcagc cacgctgcac tacaacccga
     3181 tcgcactcgt attgtgttta acgctaatga taaagctacc agtctgcggg tggataatcg
     3241 tagcgataaa ctcccctacc tcgcttattc ctggctcgaa aatgaaaaag gggagaaaag
     3301 cgatgatctt ctggtcgccc tgccgccgat acaacgtctg gaaccgaaag caacaacgca
     3361 ggtacggata gtaaagcaag cctcgaccac aaaattaccg ggcgatcgcg aaacgctatt
     3421 tttctacaat atgcgtgaaa ttcctcccgc cccggaaaaa aacagcgacc atgccgttct
     3481 gcaagtcgcc attcagagtc gtatcaaggt gttctggcga ccggctgcat tacgcaagaa
     3541 agcaggagaa aaggtcgaac tgcagttaca ggtcagccag cagggcaacc aactgacact
     3601 gaaaaatcct accgcgtatt atctgaccat tgcctacctg ggacgaaatg aaaaaggcgt
     3661 tctccccggc ttcaaaaccg tgatggttgc gccttttagt acggtcaaca caaacaccgg
     3721 aaattatagc ggcagccagt tttatctcgg ttatatggat gattacggcg cgttacgcat
     3781 gacgacgctc aactgcagcg gacaatgcca tttacaagcc gtggaggcga agaaatgagt
     3841 gctggcaagg gagtgttgct cgtcatctgt ctgttatttc tgccgttgaa gtctgcgctt
     3901 gccctgaact gctactttgg ttcatccggt ggctcggtag aaaaatcaga agcgattcaa
     3961 ccttttgctg taccaggcaa tgccaaactc ggcgataaga tttgggaatc tgacgatatt
     4021 aaaattcccg tctattgtga caacaatacc aacggcaatt ttgaaagtga gcatgtttat
     4081 gcctgggtaa acccctaccc gggggtgcag gatcgctatt atcaactcgg cgtaacttac
     4141 aatggcgttg attatgacgc caaccaggga aaaagccgca tcgacactaa ccagtgtatt
     4201 gacagtaaga acattgacat ttatacccct gagcagatta tcgcgatggg atggcagaac
     4261 aaaatttgct cgggtgatcc cgctaatatt cacatgtcgc gtacctttct cgctcgcatg
     4321 cggttatacg tcaaaatacg agaaatgccg ccgcatgatt atcaaagtac gcttagcgac
     4381 tatatcgtcg tgcaatttga cggtgccggt agcgttaacg aagatcctac tgcccaaaac
     4441 ctgaaatatc atattaccgg tctggaaaac attcgcgtgc tggattgcag cgtcaatttt
     4501 tccatttcac cggaaacaca ggtgattgat tttggtaaat ttaatttgct ggatattcgt
     4561 cgccacacaa tgtcgaaaac attcagtatt aagacgacaa aatcacaaaa tgaccaatgc
     4621 accgacggat ttaaagtcag ttcctccttt tataccgaag aaacgttggt tgaagaagat
     4681 aaagcattgt tgattggtaa tggtctgaag ctacgtttgc tggatgaaaa cgcctcgcct
     4741 tataccttca ataaatatgc tgaatacgcc gatttcacca gcgacatgtt ggtctacgaa
     4801 aaaacctata cagctgaact ttcgtccatt gcaggtaccc ccatcgaggc tgggcccttc
     4861 gatacagtgg tgctttttaa aattaactat aactgatttg ccatgatgcc ctttgcagcc
     4921 ggaaagggca tctctttcag aaaattgctg attttcccct tttccttgat tgctgttatt
     4981 ttttcctggc ccggctttct ttatgctgta tcgatacacc aaagagaaaa taatgaggga
     5041 gcgaaggatg ccagttttgc agtggggaat gttatgtgtg ctatcacttc tcctttctat
     5101 tggtttcctc gcggtacatc tcccggcggc gctattactt gggccgatga tcgccgggat
     5161 catcttcagt atgcgcggaa ttactctgca acttccccgc tccgcttttc tcgccgcgca
     5221 ggccattctt ggctgcatga ttgcgcaaaa cctcactggt tcgattctca ccacgctggc
     5281 cgccaactgg ccgatcgtgc ttgctatttt actggtgacg ctgctttcca gcgccatcgt
     5341 gggttggtta ttggtgcgct atagctcact gcctggaaat accggtgcct ggggttcttc
     5401 tccaggcggc gcagcggcaa tggtcgctat ggcgcaagat tacggcgcag atattcgcct
     5461 ggtggcgttt atgcaatatc tgcgagtgct gttcgtcgcg ggggccgctg ttctggtcac
     5521 ccgcatgatg ctgggtgata acgctgaagc ggttaaccag caaatcgtct ggttcccgcc
     5581 agtgagcatt aatctcctgc ttacgatttt gctcgcagtc gttgccggta cggcgggatg
     5641 catgctgcgt cttccttccg gcacgatgct catcccgatg ctggcgggcg cagtgctcca
     5701 gtctggtcag ctcatcacca tcgaactacc ggaatggctg ctggcgatgg cgtatatggc
     5761 aattggctgg cggattggtc ttggtttcga taaacaaatc ttactgcggg cactacgccc
     5821 actaccgcaa atcctgctgt cgatttttgc tctgctggct atttgtgcag gtatggcgtg
     5881 gggactgacc cggtttatgc atattgattt tatgactgcc tacctcgcca ccagtcccgg
     5941 cgggcttgat accgtggcgg tcatcgccgc agggagcaat gccgatatgg cgcttattat
     6001 ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg gggccagcca ttgcgcggtt
     6061 tatttcaacc tatgcgccga agcggtcggc ctagtgctgg cagcctggac accagtaaaa
     6121 cgggcgcgag gacaacgtgg ttttctcaat gatgccgcca cagcgttcgc acagttcgcc
     6181 atcacgatga aaaaccttaa agcgaaacag cgccccatga tgcttattct catccacctg
     6241 cccccgcgta gcgtaagaaa accgaggaat ctccagtaac gcgtgtgcga gtgcatccag
     6301 ttgcgccgca ttgagatctt tcgctttatg atttccagtc aacccaacct gccagaggat
     6361 ctccacccgc aaataattgc caagcccagc cagaaacgcc tgatcgagca gtaatcctgc
     6421 aaactgacgg ttacgaaagc gcggtgacaa taaccgttcc tttaccacct ccggcgtcag
     6481 attcggatcc agcacatcgg gaccaacgcg ttgtaaaaac ggatgcgtgg tcagttgttc
     6541 cggggtcaac atctcaatat ccgaggcgct ataaagcaga atggttttgt cagccgtttg
     6601 cagttttacc cgcaatactc gcgtggtctg cggctcttcg ccggtatcaa ccacgcgcca
     6661 gacgccgtaa agctgattat ggctgtagag cgttaagtcg ttggaaaaat gagttaacaa
     6721 cgccttacca cgcgtttcca catgggtaac gtgttgaccg ataagttgtg attgataagt
     6781 ttttaactgc gggaaggcaa accagacatc agttagtggt ttgcctttga tcgccgcctc
     6841 caggttatcc gctgcacggc ggatctccgg gccttcaggc atggtgatat ccttttttca
     6901 ttctttatta agcggggtta tgctgcgaca acaatcccct ttttggcaaa tgtagctcgt
     6961 aatcggcggg cgaacgccag agcgtgctcg ccgtcgccat gcaggcagac cgtttgcgcc
     7021 gcgaccgttg cccattcgcc ggtgatactt ttgactctgc cgtgttgcac catctccagc
     7081 gtttgcgcca gcgcctgttg ttcgttttca atcaacgcgc ctcgctgact tcgcggcacc
     7141 agcgagccgt cagcctgata accgcgatcg gcaaacactt cctcgcgcgt tgtcaggcca
     7201 tattgcttgc ctgcacgaat cagctcgctt cctgccagcc caacgagaat caatgccgga
     7261 tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt gtgcttcttt cgccgcctgg
     7321 ttgtacaaca tgccgtgcgg tttgacatga cgcattacgc cgccttgcgc acgggcaatc
     7381 gttgccagcg cgccaatttg atacagcgtc tgggcgtaaa cggtttctgg cggcagctgc
     7441 atggcgctgc gaccaaaatt ttccctgtcg ggaaaactcg ggtgagcgcc aattgcaaca
     7501 ccatttttta ttgcttcacg cacgcaagca tgcatggttt gcgcatcgcc tgcatgaaat
     7561 ccacaggcaa tattggcaga ggaaaccagc gttaatagct ctgcgtcgct ggcgcagcct
     7621 tcgcccagat cggcgttcag gtcaattttc attgtgcagc cgccacgcta attgttcaaa
     7681 ataacgttgc tgatcttgcc gcgctttcag tgcctcttcc agtgaacact ggacaaaatg
     7741 aatcggctga ccgagcggaa tttgcgccag atggtacata tcagcctcaa tgatgcaggc
     7801 aatacgcggg taaccaccgg tggtctgtgc gtcgttcatc aacacaatcg gctgcccgtt
     7861 atgtggcacc tgcaccacgc ccggtaacaa accgtgagat aacagttcgc gatcggtggt
     7921 gcgttttaaa atttgcccct gtaagcgata gcccatgcgg ttactttgcg ggctaagctg
     7981 ccagggcgaa cgccagaatg catcctgcga ggcgcgatcg aactcatgat attccggccc
     8041 cggcaaggcg cgaatgcggt tgccccacag cagctgttta acgccctgcg cttccataaa
     8101 atcacgcttc gatttgccaa tcgggagtcg gtcaccatcc ttcagtaaac ggccttccag
     8161 cccgccaatc cccacgttga gatcggtgct gcatgacccc attaccggcg gaacatcaat
     8221 accacccgcg accgccagat aactgcgcat cccgtgctgc gggcgtttaa gcgttaaacg
     8281 ctggcctgct ttcatcggca atcgccagcc ggtccagacg gcattatcat ccagccgcgc
     8341 ttcgcaaccg gcacccgtca gagcaaacca cccatcagtt tcgaactcaa cagttaactg
     8401 accgagcgtg atctccagcg cgggggcatt ggcgtcatta cccaccagta ggttagcaat
     8461 gcgtaacgcg ggcatatcca gtgcgccgca gtggctgata cccgactggc gaaaaccgtg
     8521 acgaccgcca tcctgcacag tggtatacat gcccgcacga ataatcttca gcatactccc
     8581 tccttctgcg gtacaaagcg cacgctgtct cccggacgta ataagatggg ttcgtcacgc
     8641 gccggatcaa acaggctgag tgaggtatga ccaatcaact gccagccacc cggcgttgcc
     8701 agcggataaa caccagtctg cggcccgccg atcccgacag aacctgccgg aacgagtaag
     8761 cgcggttcag cgcgccgtgg cgtgtgtagt tgttccggca aactcccgag atacgggaag
     8821 cccggttgaa aacctaaaaa ccagaccacg tattccacgg aggagtgcaa ttcaacaacc
     8881 tgtttttcgc tcaacccgca atgcgccgcg accaccgcca aatccggtcc gcctgcacca
     8941 ccgtaaacca ccggaatttc aataaagcga gactcaggtt ccagggcctc gctctcctcc
     9001 caccagcgtt gcaaacgctc tatggcatcc agcgccagcg actcaggatt acgcaaaatc
     9061 accgtaatat tgttcatgcc gggaatggct tcaaccacat tcggcatatc caccagacgc
     9121 tgcgccagtc gccagatccg tttctggcta gccagcgtca ccggcggttc cagttccagc
     9181 actaccgccg tttcacctat cagataacaa cgcgctcgtt gcactttcga tccctctgat
     9241 tattcgttat gcaggattag gaatatcaat aaaggtcaca tcaagatcgg tattttcatt
     9301 cagccactcg ctcaatgcgc gaataccacc acgttcagtg gcatggtgac ctgcagcata
     9361 aaaatgcaat ccctgctcgc gggctgaatg aatggtctgt tcagaaactt cgccagtaat
     9421 aaaagcatcc acgccaaaac gcgcggcgct atcgataaaa ctttgcccgc cgcccgtgca
     9481 ccaggcgacg cgctggacca cctcaggtcc ggtatcgcca caccataacg gcttacgtcc
     9541 cagacgcgct tcaatccagg aggctagttc cagtcccggc acaggcatgg tcagctctcc
     9601 ccacggcacc aacggctcaa tttcgcccat gaccgtgatc cccagtaacg ccgccagctg
     9661 tgcgttattg cccagctcag gatgtgcgtc aagcggcaaa tgccagccat acaggttgat
     9721 atcattcgcc agcaacgttt ttaaacggtt acgcttcatt ccgcgaatga ccggagactc
     9781 tcctttccag aagtagccat gatgcacaat gaccgcatcc gcgcccagac gcactgcctc
     9841 atcgagcaaa gcctggctgg cggtgacacc ggtaacaatt ttttgcaccg tctctttgcc
     9901 ttccacctgc aaaccattcg gcgcgtaatc actaatcgcc gcgctgttca gtttttcgtt
     9961 aatcagttgt tccagttcgg tgtttttcat cttcgcgcct tccgtattct gatagctaca
    10021 ccgttaacat agcgttacct cgcctgttcg tcgatagcca atcttttgac atattaagaa
    10081 acaaacccga cgccaaaaga cgcaaattgt ttatatcatc acgcttcagc taatgtatat
    10141 ttatgcaatc agacagcata ttgcataaat aatcagatct gtatcaaagt tttaacttta
    10201 aggaaacaca agcacgccgc gcaactatta cgtgacgttg ccgtcaattg tggaattatt
    10261 atgaataaac acgcatcaca accacgcgct atttactatg tcgttgcgct gcaaatctgg
    10321 gaatatttta gcttttacgg catgcgtgcc ctgctgattc tctatctcac caatcaacta
    10381 aaatacaacg atactcacgc ctacgagtta tttagcgcct actgttcgct ggtgtatgtc
    10441 acgccaatcc tcggtggctt tttggcggat aaagttctcg gcaatcgcat ggcggtgatg
    10501 ctgggggcgt tgttgatggc gattggtcat gtggtgctgg gtgccagtga gatccatccg
    10561 tcattcctct atctgtccct ggcgattatc gtctgcggct atggcctgtt taaatctaac
    10621 gtaagctgtc tgctcggcga gctgtatgag ccaaccgatc cgcgtcgtga tggcggtttc
    10681 tcgctgatgt atgcggcggg taacgtgggg tctattatcg cacctatcgc ctgtggttac
    10741 gcccaggaag agtacagctg ggcgatgggc tttggcctgg cggcggttgg catgatcgcg
    10801 ggtctggtca ttttcttatg tggcaatcgt catttcactc atacccgcgg cgttaacaaa
    10861 aaagtactgc gtgcgacaaa ctttctcctg ccgaactggg gatggctgct ggttctgctg
    10921 gtggcaacgc ctgcactgat taccgtactg ttctggaaag agtggtcggt atacgcctta
    10981 attgtcgcga ctattattgg cctgggtgta ctggcaaaaa tttatcgcaa agcagaaaac
    11041 cagaaacagc ggaaggagct ggggctgatt gtgacgctca ccttcttcag tatgttgttc
    11101 tgggccttcg cacaacaggg cggtagctcg attagccttt atatcgaccg cttcgttaac
    11161 cgcgatatgt ttggt
//
LOCUS       DAJOWB010000135        11139 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJOWB010000135 DAJOWB010000000
VERSION     DAJOWB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.135"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(147..1811)
                     /gene="glnS"
                     /locus_tag="OHO85_004004"
     CDS             complement(147..1811)
                     /gene="glnS"
                     /locus_tag="OHO85_004004"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCQ2673309.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2079..4025)
                     /gene="nagE"
                     /locus_tag="OHO85_004005"
     CDS             complement(2079..4025)
                     /gene="nagE"
                     /locus_tag="OHO85_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCQ2673310.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVRAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4358..5158
                     /gene="nagB"
                     /locus_tag="OHO85_004006"
     CDS             4358..5158
                     /gene="nagB"
                     /locus_tag="OHO85_004006"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCQ2673311.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5218..6366
                     /gene="nagA"
                     /locus_tag="OHO85_004007"
     CDS             5218..6366
                     /gene="nagA"
                     /locus_tag="OHO85_004007"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCQ2673312.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6375..7595
                     /gene="nagC"
                     /locus_tag="OHO85_004008"
                     /gene_synonym="nagR"
     CDS             6375..7595
                     /gene="nagC"
                     /locus_tag="OHO85_004008"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCQ2673313.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7643..8395
                     /gene="nagD"
                     /locus_tag="OHO85_004009"
     CDS             7643..8395
                     /gene="nagD"
                     /locus_tag="OHO85_004009"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCQ2673314.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8792..10456
                     /gene="asnB"
                     /locus_tag="OHO85_004010"
     CDS             8792..10456
                     /gene="asnB"
                     /locus_tag="OHO85_004010"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCQ2673315.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVTDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10835..10911
                     /locus_tag="OHO85_004011"
     tRNA            10835..10911
                     /locus_tag="OHO85_004011"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10869..10871,aa:Met,seq:cat)
     gene            10920..11004
                     /locus_tag="OHO85_004012"
     tRNA            10920..11004
                     /locus_tag="OHO85_004012"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10954..10956,aa:Leu,seq:tag)
     gene            11028..11102
                     /locus_tag="OHO85_004013"
     tRNA            11028..11102
                     /locus_tag="OHO85_004013"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11060..11062,aa:Gln,seq:ttg)
BASE COUNT         2773 a         2890 c         2816 g         2660 t
ORIGIN      
        1 tacaaaagca cgcaaactca atatattgca gagatcatgt aggcctgata agcgtagcgc
       61 atcaggcaat ttagcgtttg aaactgtttg tctgttcctt gccggatgcg acgtaaacgc
      121 cccatccggc atagcgaaac ttaaaattac tcgcctactt tcgcccaggt atcacgcagc
      181 ccaacggtgc ggttaaatac cggtttttcc gccgtagaat ggcggctatc gaggcagaag
      241 taaccttcac gctcaaactg gaatgcttta cccgcaaccg catctttcag cgacggttca
      301 gcaaagccct gtttgatcac cagcgattcc gggttaatca ccgacaggaa atcatccgca
      361 gcacctgggt taggcacgct gaacagacgg tcatacaaac ggatttcaac cggcagcgca
      421 tgtgccgcgc tcacccagtg aataacacct ttgactttac gaccatctgc cggatctttg
      481 cttaaggtat cggcgtcata agtacagaag atggtggtga tattaccttc ggcatctttc
      541 tcgacgcgtt ctgccttaat cacataagca ttacgcagac gcacttcttt acccagcacc
      601 agacgtttgt actgcttgtt agcttcttcg cggaaatcgg cgcgatcaat ccaaatctca
      661 ccgctaaacg gcacctgacg gctgcccatt tccggtttgt tcggatggtt cggcatggta
      721 accatttcgc cttcgccctg atagttttcg ataaccagtt tcaccggatc gataaccgcc
      781 attgcgcgcg gcgcattttc gttgagatct tcacggatgc aggattccag cgacgccatc
      841 tcaatggtgt tgtcctgctt ggtcacgccg atgcgtttgc agaactcacg aatagaagcc
      901 gcagtgtaac cacgacgacg cagaccggaa atggtcggca tacgcgggtc atcccagcct
      961 tcaacgtgct tgtcggtcac cagcaggttc aacttacgct tggacatcac ggtgtattcc
     1021 agattcaggc gcgagaactc atactggcgc gggtgaacag gaatcgtgat gttgtccagt
     1081 acccagtcgt acagacgacg gttgtcctgg aactcaagcg tacacagaga gtgcgtaata
     1141 ccttccaggg catcgctgat gcagtgggtg aagtcgtaca tcgggtagat gcaccacttg
     1201 ttgccagtct ggtggtgttc agcaaattta atacggtaca gcaccggatc gcgcatcacg
     1261 ataaacggtg aagccatgtc gattttcgca cgcaggcagg ctttaccttc ttcaaaacca
     1321 ccggcacgca ttttttcgaa cagcgccagg ttctcttcaa cgctgcggtc gcggtacggg
     1381 ctgtttttac ccggttgcgt cagggtgccg cggtattcgc ggatctgttc cggcgtcagt
     1441 tcatcaacgt acgccaggcc tttattgatc agttcgatcg cataggcgtg gagctgatca
     1501 aaataatcgg aggagtaacg gacgttacca gaccagtgaa aacctaacca ctctacgtcg
     1561 tttttgatcg actcaacata ctcgatatct tcttttaccg ggttagtgtc gtcgaaacgc
     1621 aggttgcact ggcctttata gtcctgggcg atcccgaagt tcaggcagat agatttcgca
     1681 tggccaatat gcagatagcc attcggctcc ggcgggaaac gggtgtgtac tgtggtgtgc
     1741 ttaccactgg ccagatcttc atcgatgatc tgacggataa agttagtcgg gcgggcttct
     1801 gcctcactca tcgtggattc ctcaaagcgt aaacaacgta taacggcgta tgatcttata
     1861 agcgggacag gctgacaact gttagttttt taaaattgat aatctaacaa ggattatggg
     1921 gatgattgat gaaaaaaaaa gcggcagaga agatctccac cgcttttgcc agtcctgcgt
     1981 gattgtcgga tgcaacgcta aagcgtctta tccaacctgg actgagcttt tgtaggcctg
     2041 ataagacgcg tcaagcgtcg catcaggcat gaagcagatt actttttgat ttcatacagc
     2101 ggtgtttgac ccgccacaac atggccctga gctttaatga tcaagccact gaaatcgtcg
     2161 atattgctgc aaaccaccgg actaatcatc gagcgggcgt tagcgttcag gtaatccaga
     2221 tccatttcca gaatcggttg ccctgctctt acctgcgcgc cctcttccac cagacgttta
     2281 aagcctttac cttccagcgc tacggtgtcg atacccatgt ggacgacgat ctccgcgcct
     2341 ttttcggttt ccagacagaa cgcgtggttg gtgttgaaga ttttcacgat tgtccctgcg
     2401 gctggtgata cgacgatttt atctgtcggt ttcaccgcca caccgtcacc caccgctttg
     2461 ctggcgaatg cttcgtcagg aacctgatcc agtgccacga catcaccggt aatcggcgat
     2521 accagctccg cgatagatac cgcgtttggt acagcctgcg gttttgctac aggagcggca
     2581 gttgccggag ttgcttcagc tgacgcagct gctaccgggc cacgggcaac gactttcttc
     2641 atcgcatcgc caatggattc tgctttcgcg ccaacaatca cctgaatggt ctgtttgttc
     2701 agttttacta caccagaagc acccagacgt ttacacatcg catcgttgac gcgagctgag
     2761 tcagccacgg taaggcgcag acgggtaata caggcgtcaa tcgctttcag gttgtcagta
     2821 ccgccaaccg cagcaatata gttggttgcc agttgagtca gaccttcttc agtattgctg
     2881 ttagcctctt cagttacgat ctcgtcttct ttatcttcac gacccggcgt tttcagattg
     2941 aacatgcgga taaccaaact gaacaccacg aagtagatag cgaagaagac aacacccata
     3001 accagcagca tccagacgtt ctggctggcg gccggcaggt tatacatcaa cgcgtagtcg
     3061 atagcacccg cagagaaaga gaagccagca tggataccca gcagcgttgc cacaaacagg
     3121 ctgataccgg tcagcagtgc gtgcaggagg tacagcagcg gtgcaaggaa catgaacagg
     3181 aattccagcg gctcagtcac accggtcagg aacgcagtaa cagcaacaga aagcagcatc
     3241 ccgccaacca tcggacgacg ctctttcggt gctgcgaagt acatcgccag cgccgcaccc
     3301 ggcagaccga acatcatgat cgggaagaag ccggacatga acatccccgc ggtgccgtca
     3361 ccagcgtaga aacggttgat gtcgccgtgg aaaaccgtac ccgccgcgtt ggtgaattca
     3421 ccaatctgga accaggcgat ggtgttcagc acctgatgca gaccggttgg gatcagcaga
     3481 cggttgatga aaccaaagat accggaaccc agcgcgcccg cagaaacgat ccactcgccg
     3541 cctgcatgga tagcgtgctg taccggcggc cagacgtaac caaaaatggc cgccagcacc
     3601 agacagaaga agccggtggc gatcggcaca aagcgtttgc cgccgaagaa gctcaggaag
     3661 tccggcagtt taatatcgga ccaacggtta taggctgcgc caccaaccag accggtaatg
     3721 atacccgcca gtacacccat gttaatttct gggttgatgg tcaccatcgc tttggttaac
     3781 acaaagtaac ctaccgcacc cgccagcgcc gccgcacctg cgctgtcttt cgaccagctg
     3841 gatgccacac cgatggcgaa gattaatgcg aggttatcaa aaatcgcacc gcccgcctgg
     3901 gcaataaacg caacgttaag taaatctggc tgaccgaatc gcagcaacag tgccgccacc
     3961 ggcagcaccg cgatagggag ctgtaacgcc ctaccgagtc gctggaaaaa acctaaaata
     4021 ttcatcttat tccccctacg agaaccctat ttggctcgtt taaagccgta tttttatttt
     4081 gctgcaaatt gtactgccga tgttctgtaa tcagattgtt agatcatctg ctacagagtg
     4141 tgtgaaaatt taattcgtat cgcaaattaa atgcgtgtct tttgtgagtt ttgtcaccaa
     4201 atatcgttat catcactccc ttttactggc taaaccagaa aacttatttt atcattcaaa
     4261 aaatcaggtc ggattgacgc ctgtctgcgc aaatccaggt tacgcttaaa gatgcctaat
     4321 ccgccaacgg cttacatttt acttattgag gtgaataatg agactgatcc ccctgactac
     4381 cgctgaacag gtcggcaaat gggctgctcg ccatatcgtc aatcgtatca atgcgttcaa
     4441 accgactgcc gatcgtccgt ttgtactggg cctgccgact ggcggcacgc cgatgaccac
     4501 ctataaagcg ttagtcgaaa tgcataaagc aggccaggtc agctttaagc acgttgtcac
     4561 cttcaacatg gacgaatatg tcggtctgcc gaaagagcat ccggaaagct actacagctt
     4621 tatgcaccgt aatttcttcg atcacgttga tattccagca gaaaacatca accttctcaa
     4681 cggcaatgcc ccggatatcg acgccgagtg ccgccagtat gaagaaaaaa tccgttctta
     4741 cggaaaaatt catctgttta tgggcggtgt aggtaacgac ggtcatattg catttaacga
     4801 accggcgtct tctctggctt ctcgtactcg tatcaaaacc ctgactcatg acactcgcgt
     4861 cgcgaactct cgtttctttg ataacgatgt caatcaggtg ccaaaatatg ccctgactgt
     4921 cggtgttggt acattgctgg atgccgaaga agtgatgatt ctggtgctgg gtagccagaa
     4981 agcactggca ctgcaggctg cagttgaagg ttgcgtgaac cacatgtgga ccatcagctg
     5041 tctgcaactg catccaaaag cgatcatggt gtgcgatgaa ccttccacca tggagctgaa
     5101 agttaagact ttaagatatt tcaatgaatt agaagcagaa aatatcaaag gtctgtaatt
     5161 gttatccctg ccctggctcc ttgctcaggg caatattttt taaaatcggg ggtcagaatg
     5221 tatgcattaa cccagggccg gatctttacc ggccacgaat ttcttgatga ccacgcggtt
     5281 gttatcgctg atggcctgat taaaagcgtc tgtccggtag cggaactgcc gccagagatc
     5341 gaacaacgtt cactgaacgg ggccattctc tcccccggtt ttatcgatgt gcagttaaac
     5401 ggctgcggcg gcgtacagtt taacgacacc gctgaagcgg tcagcgtgga aacgctggaa
     5461 atcatgcaga aagccaatga gaaatcaggc tgtactaact atctgccgac gcttatcacc
     5521 accagcgatg agctgatgaa acagggcgtg cgcgttatgc gcgagtacct ggcaaaacat
     5581 cccaatcagg cgttaggtct gcatctggaa ggtccgtggc tgaatctggt aaaaaaaggc
     5641 acccataatc cgaattttgt gcgtaagcct gatgccgcgc tggtcgattt cctgtgtgaa
     5701 aacgccgacg tcattaccaa agtgaccctg gcaccggaaa tggttcctgc ggaagtcatc
     5761 agcaaactgg caaatgccgg gattgtggtt tctgccggtc actccaacgc gacgttgaaa
     5821 gaagcgaaag ccggtttccg cgcggggatt acctttgcca cccatctgta caatgcgatg
     5881 ccgtatatta ccggtcgtga accgggcctg gcgggcgcga tcctcgacga agctgacatt
     5941 tattgcggta ttatcgctga tggcctgcat gttgattacg ccaacattcg taacgctaaa
     6001 cgccttaaag gcgacaaact gtgtctggtt accgacgcca ccgcgccagc aggtgccaac
     6061 attgaacagt tcatttttgc gggtaaaaca atatactacc gtaacggact ttgtgtggat
     6121 gagaacggta cgttaagcgg ttcatcctta accatgattg aaggcgtgcg taatctggtc
     6181 gaacattgcg gtatcgcact ggatgaagtg ctgcgtatgg cgacgctcta tccggcgcgt
     6241 gcgattggcg ttgagaaacg tctcggcaca ctcgccgcag gtaaagtagc caacctgacc
     6301 gcattcacac ctgattttaa aatcaccaag accatcgtta acggtaacga ggtcgtaact
     6361 caataagaga aagtatgaca ccaggcggac aagctcagat aggtaatgtt gatctcgtaa
     6421 aacagcttaa cagcgcggcg gtttatcgcc tgattgacca gtacgggcca atctcgcgga
     6481 ttcagattgc cgagcaaagc cagcttgccc ccgccagcgt aaccaaaatt acgcgtcagc
     6541 ttatcgaacg cgggctgatc aaagaagttg atcagcaggc ctccaccggg ggccgccgcg
     6601 ctatctccat cgtcaccgaa acccgcaatt tccacgcaat cggcgtacgg cttggtcgtc
     6661 atgacgccac catcactctg tttgatctca gcagcaaagt gctggcagaa gaacattacc
     6721 cgctgccgga acgtacccag cagacgctgg aacatgccct gctgaatgcc attgctcagt
     6781 ttattgatag ctaccaacgc aagctgcgcg agctgatcgc catttcggtg atcctgccag
     6841 ggcttgttga cccggacagc ggcaaaattc attacatgcc gcatattcag gtagaaaact
     6901 gggggctggt agaagcactg gaagaacgtt ttaaagtgac ctgtttcgtt ggtcacgata
     6961 tccgtagtct ggcgctggcg gagcactact tcggtgcaag tcaggattgc gaagactcca
     7021 ttctggtgcg tgtccatcgc ggaaccgggg ccgggattat ctctaacggg cgcattttta
     7081 ttggccgcaa cggcaacgtc ggtgaaattg gccatattca ggtcgaaccg ctgggtgaac
     7141 gctgccactg cggcaacttt ggctgcctgg aaactatcgc cgccaacgct gccattgaac
     7201 aacgggtgtt gaatctgtta aagcagggct accagagccg tgtgccactg gacgactgca
     7261 ccatcaaaac tatctgcaaa gccgcgaaca aaggcgatag cctggcctcg gaagtgattg
     7321 agtatgtcgg tcgtcatctg ggcaaaacca tcgccattgc tatcaactta tttaatccgc
     7381 aaaaaattgt tatcgctggt gaaatcaccg aagccgataa agtgctgctc cctgctattg
     7441 aaagctgcat taatacccag gcgctgaagg cgtttcgcac taatctgccg gtggtacgtt
     7501 ctgagctgga tcaccgctcg gcaatcggcg cttttgcgct ggtaaaacgc gccatgctca
     7561 acggtatttt gctccagcat ttgctggaaa attaatgtgc ttttatagtg gcgcttattg
     7621 ttgtcaatat tctgggtagt ccatgaccat taaaaatgta atttgcgata tcgacggcgt
     7681 gctgatgcac gataacgtcg ccgtaccggg tgcagcggaa tttttgcacg ggattatgga
     7741 taaaggcctg ccgctggtgt tgctgaccaa ctatccttcg cagaccgggc aggatctggc
     7801 gaaccgcttt gccactgccg gtgtcgatgt acctgacagc gtgttttata cctctgcgat
     7861 ggcgaccgcc gatttcctgc gtcgccagga aggcaagaaa gcgtatgtgg tgggcgaagg
     7921 cgcactgatt catgaactgt acaaagccgg tttcactatt accgatgtga accctgattt
     7981 tgtgattgtt ggcgaaacgc gttcctacaa ctgggacatg atgcataaag cggcctattt
     8041 cgtcgctaac ggtgcacgtt ttatcgccac caacccggac acccacgggc gcggttttta
     8101 tcccgcctgt ggcgcgttgt gtgcagggat cgaaaaaatc tccgggcgta aaccgttcta
     8161 tgttggtaag ccgagtccgt ggatcatccg cgcggcatta aacaaaatgc aggcgcattc
     8221 ggaagaaacg gtgattgtcg gcgataacct gcgtaccgat attctggccg gcttccaggc
     8281 aggtctggag acgattctgg tgctttctgg tgtttcgtcg ctcgacgata tcgacagtat
     8341 gcctttccgc cccagctgga tttacccgtc agtcgctgaa atcgacgtta tctgaatagc
     8401 aaccacgtct caggacgtgg ttggctctga tgacaaacgc caaactgcct gatgcgctac
     8461 gcttatcagg cctacgcagc tcctgcaata tattgaattt tcatgctttt gtaggccgga
     8521 taaggcgttc acgccgcatc cggcatgaac aaatcgcact ttgtcagcaa tttggccccc
     8581 gtaaaatata aacattcaat aaaacccgct aaaaattatc gattcatcaa taaatatatc
     8641 ctcacaccag accttttttc accaatcgac aatcaccatt acgtttttta ttttttcacc
     8701 gccaaatcgc ttgcgcacag tgctgctttg cggcattttt ttaaacaagc aaacacaaca
     8761 agcaacaaat acaaggttaa cggagaaggt tatgtgttca atttttggcg tattcgatat
     8821 caaaacagac gcagttgagc tgcgtaagaa agccctcgag ctgtcacgcc tgatgcgtca
     8881 tcgtggcccg gactggtccg gtatttatgc cagcgataac gccattctcg cccacgaacg
     8941 tctgtcaatt gttgacgtta acgcgggggc gcaacctctc tacaaccaac aaaaaaccca
     9001 cgtactggcg gtaaacggtg aaatctacaa ccaccaggca ttgcgcgccg aatatggcga
     9061 tcgttaccag ttccagaccg ggtctgactg tgaagtgatc ctcgcgctgt atcaggaaaa
     9121 agggccggaa tttctcgacg acttgcaggg catgtttgcc tttgcactgt acgacagcga
     9181 aaaagatgcc tacctgattg gtcgcgacca tctggggatc atcccactgt atatggggta
     9241 tgacgaacac ggtcagctgt atgtggcctc agaaatgaaa gcgctggtgc cagtttgccg
     9301 cacgattaaa gagttcccgg cggggagcta tttgtggagc caggacggcg aaatccgttc
     9361 ttactatcat cgcgactggt tcgactacga tgcggtgaaa gataacgtga ccgacaaaaa
     9421 cgagctgcgt caggcactgg aagattcagt taaaagccat ctgatgtctg atgtgcctta
     9481 cggtgtgctg ctttctggtg gtctggattc ctcaattatt tccgctatca ccaagaaata
     9541 cgcagcccgt cgcgtggaag atcaggaacg ctctgaagcc tggtggccgc agttacactc
     9601 ctttgctgta ggtctgccgg gttcaccgga tctgaaagca gcccaggaag tggcaaacca
     9661 tctgggcacg gtgcatcacg aaattcactt cactgtacag gaaggtctgg atgccatccg
     9721 cgacgtgatt taccacatcg aaacttatga tgtgaccact attcgcgctt caacaccgat
     9781 gtatttaatg tcgcgtaaga tcaaggcgat gggcattaaa atggtgctgt ccggtgaagg
     9841 ttctgatgaa gtgtttggcg gttatcttta cttccacaaa gcgccgaacg ccaaagaact
     9901 gcatgaagag acggtgcgta aactgctggc cctgcatatg tatgactgcg cccgtgccaa
     9961 caaagcgatg tccgcctggg gcgtggaagc gcgcgttccg ttcctcgata agaaattcct
    10021 tgatgtggcg atgcgtatta acccacagga taaaatgtgc ggtaacggca aaatggaaaa
    10081 acacatcctg cgtgaatgtt ttgaagcgta tctgcctgca agcgtggcct ggcggcagaa
    10141 agagcagttc tccgatggag tcggttacag ttggatcgac accctgaaag aagtggcggc
    10201 gcagcaggtt accgatcagc aactggaaac tgcccgcttc cgcttcccat acaacacgcc
    10261 aacctctaaa gaagcgtatc tgtaccggga gatctttgaa gaactgttcc cgcttccgag
    10321 cgccgctgag tgcgtgcctg gcggtccttc cgtcgcgtgt tcttccgcta aagcgatcga
    10381 atgggatgaa gcgttcaaga aaatggacga tccatctggt cgtgcggttg gtgttcacca
    10441 gtcggcatat aagtaagaaa tgcgaaacag ccccggtgaa atccggggct gtttcagtta
    10501 ttgaggtgct tgttgattct gatatgcagt aaggaaatgt tccgggtttg ccacatcact
    10561 gtttgacaac tcatcactgt aggccagtat ttcatagatt aatttcccac cactattgtt
    10621 gaattccgaa tttcatcctg tatgacaaaa gaatggctgt agggtgggaa aaaacacaaa
    10681 gtaaacaata attgacgaat atagcgccac gctgttcgca acctaaccaa acagtcactt
    10741 tcgagcaatt ttccttgaaa aagaggttga cgctgcaagg ctctatacgc ataatgcgcc
    10801 ccgcaacgcc gataaggtat cgcgaaaaaa agatggctac gtagctcagt tggttagagc
    10861 acatcactca taatgatggg gtcacaggtt cgaatcccgt cgtagccacc atcttttttg
    10921 cgggagtggc gaaattggta gacgcaccag atttaggttc tggcgccgca aggtgtgcga
    10981 gttcaagtct cgcctcccgc accattcacc agaaagcgtt gtacggatgg ggtatcgcca
    11041 agcggtaagg caccggtttt tgataccggc attccctggt tcgaatccag gtaccccagc
    11101 catcttcttc gagtaagcgg ttcaccgccc ggttattgg
//
LOCUS       DAJOWB010000136        11054 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJOWB010000136 DAJOWB010000000
VERSION     DAJOWB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.136"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(356..2038)
                     /gene="glcA"
                     /locus_tag="OHO85_004014"
     CDS             complement(356..2038)
                     /gene="glcA"
                     /locus_tag="OHO85_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HCQ2673316.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
     gene            complement(2393..4564)
                     /gene="glcB"
                     /locus_tag="OHO85_004015"
     CDS             complement(2393..4564)
                     /gene="glcB"
                     /locus_tag="OHO85_004015"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCQ2673317.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQLERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGAQVIAWVRRFLDESLPLEKGSYQDVVAFKVVDKQLRIQLKNVKETTLRTPAQFVG
                     YRGDTAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRVETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMFSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4586..4990)
                     /locus_tag="OHO85_004016"
     CDS             complement(4586..4990)
                     /locus_tag="OHO85_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HCQ2673318.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(4995..6218)
                     /gene="glcF"
                     /locus_tag="OHO85_004017"
     CDS             complement(4995..6218)
                     /gene="glcF"
                     /locus_tag="OHO85_004017"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HCQ2673319.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQLVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            complement(6229..7281)
                     /gene="glcE"
                     /locus_tag="OHO85_004018"
     CDS             complement(6229..7281)
                     /gene="glcE"
                     /locus_tag="OHO85_004018"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCQ2673320.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7281..8780)
                     /gene="glcD"
                     /locus_tag="OHO85_004019"
     CDS             complement(7281..8780)
                     /gene="glcD"
                     /locus_tag="OHO85_004019"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HCQ2673321.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            9026..9790
                     /gene="glcC"
                     /locus_tag="OHO85_004020"
     CDS             9026..9790
                     /gene="glcC"
                     /locus_tag="OHO85_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCQ2673322.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            complement(9797..10939)
                     /locus_tag="OHO85_004021"
     CDS             complement(9797..10939)
                     /locus_tag="OHO85_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCQ2673323.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHCERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
BASE COUNT         2641 a         3077 c         2751 g         2585 t
ORIGIN      
        1 ataagaataa cccctatttg atgtctggct ggctattttt acgaaatttc aataaacaaa
       61 aaattaacaa catttcacaa cgcaactata taaattcgtt ggtagtttta acgattaaga
      121 ttgcaaaagc aacagataga acggtgtttg ctcatcaagc gaacataatt aatacagact
      181 tgttttgcgt gataaaaaaa atagatgttc tcacgctctt aattatttag caggttatct
      241 gaacacaaaa cattattgcc gcatggataa taaagcgaga aataattttc aattaataag
      301 acagaataat gtaaatgaag ccggatgata ttaacgatca tccggcatta ttgatttacg
      361 agaccaacat tccagtaaac acatacgcct gcaacaacgt gataacgccg ataacgctgg
      421 caaaaatcag actgtgcttc acggtgtagc ggaatagttc agattctcgg cccaccatgc
      481 ccgtcgcggc gcaggccacg gcgatagatt gcggggagat catcttgcca gttacgccgc
      541 cgctggtatt tgctgccacc agcagagtgt cagagacgtt gatttgctgc gccgtggtcg
      601 attgcagtga accaaacagg gcgttagagg aggtgtccga gccggtaagg aatacgccca
      661 gccagccgag aaacggtgag aagaacggga acatcacgcc tgtacctgcc agtaccagcg
      721 ccagcgtggt ggacatgcca gaatagttgg tgacgaaggc gaacgccagc accatgccaa
      781 tcgacagtat cggccacttc aagctaatta gcgtttcggc aaagacgcca atacctttct
      841 tgatcccaac accgaggatg aagatagaga taatcgccgc aataaaaata gcggtgccgc
      901 cagccgagag agggtcgaat ttaaacaccg catccattgg cgttggttgg gcgacaatgg
      961 gtgccgcttt caacacttgt tgatgcaaat gagggatctg gaaattaatc accagtgaat
     1021 aaaacgcgcc gcccggagca aataacgctt taaacggctt catggtccag atggtgacca
     1081 gcaccgttaa gattaaaaac ggtgaccacg cacgaatgat ttgccccaga ctatattctg
     1141 aaggcacggg accgccagaa gatggcttat ttaccaccat cgcacctgcg gattgcccca
     1201 tgctgattgc cgtttcggta tttttcggcc gccagacttt aaggaataaa gcgagtgaga
     1261 cgatactcac cagcgccgaa gtaatatccg gcagttccgg accaatatag ttagaggtaa
     1321 agaactgagt gacagcaaag cttcccccag caaccagcgc cgctggccac gtctctttca
     1381 cccctttcca gccgtccatc attgctacca gccagaacgg cacaagaacc gacaggaacg
     1441 gtaactggcg tcccgccatt gcgccaatgt ggaacggatc gattcccgtt acctgaccgg
     1501 cgaccagaat cggcacgccc aacgcaccaa acgccactgg cgcagtgttg gcaatcagac
     1561 acaaccccgc cgcgtataac ggtttgaagc ccaagcccac cagcagcgca ccggtaatcg
     1621 ccaccggagc accaaagcca gccgctcctt ccagcaacgc accaaaggag aaaccaatca
     1681 gtaacacctg caaacgctga tcgtcggtga tggagataac cgagctgcgg ataatatcga
     1741 actgcccgct ggcaacggtt aatttataca ggaacaccgc cgcgacaata atccacgcaa
     1801 ttggccataa tccataaata aagccatagc ccgcagcagc aaatgccata tcaatcggca
     1861 ttttaaaggc gaatattgca atcaggatag ataatataag ggttattgct ccagcgacat
     1921 gtcctttcag acgtaatacc gcgagtgcaa cgaagaagaa tattatcggg atcagggcga
     1981 ccagagcgga tagccccagt cctcccatcg gcatatacat ttgggtccag gtaaccatat
     2041 tgttcagtct cttattatct ttatatgctt gatatactta aggttgtaat aagcaaaaga
     2101 ggactgaact gtaaaatata ggcgttatac tttacagcaa cagtacgccg ctaacgcaat
     2161 tgctacctct ggcataacaa gtatatcggg taagggtttc tgttctgcac acgcaggcgc
     2221 agagtatcgt taagatgtcc atattgttgt tttaatcccg ctagtgatgc gctacgggta
     2281 tttaatattg ttaaaccctg ataatcgctc cggttatttc cgggataaat gtactaccgc
     2341 agttactatc atagccccga caataaaact tgccggggct tttttgacgc tattaatgac
     2401 tttctttttc gcgtaaacgc caggcgtgta ataacggttc ggtataaccg tttggctgtt
     2461 tcacgccgag gaagattaaa tcgctggcag ctttaaaagc acacgagtta gcgaaattcc
     2521 ccgccatcgg acgatacgcc ggatcgccag cgttttgctg atccaccact ttcgccatat
     2581 tctccagcga cgcctgcacc tgttctttgg tcagaatacc gtgacgtaac cagttggcga
     2641 tatgctggct ggagatacgc agcgttgcgc ggtcttccat cagcgccacg ttgtgaatat
     2701 ccggcacttt tgaacaacca atcccctgct ccacccagcg caccacatac cccagaatcc
     2761 cctgcacgtt gttatccagc tcttgttgga tctcttccac cgaccagtta gcgttttcag
     2821 caaccggaat agtcagcaga tcgtccagca gcggttcaaa ttcagcattg aactcggtct
     2881 gggcaatgtt agcttgcacg ctctgtacgt tggtttggtg gtagtgcagc gcatggagcg
     2941 tagcggcagt tggcgacgga acccaggccg tgtttgcccc ggcacgcagc tggtcgcctt
     3001 tctggctgaa catgtctgcc atcaggtccg gcattgccca catgcctttg ccgatttgcg
     3061 ctttaccgcg cagcccacag aacagaccgg aaagcacgtt attacgttcg taggctttga
     3121 tccacggcgt cgatttcatc tggtttttac gcagcattgg gccagcttcc atcaccgaat
     3181 gcatctcatc gccggtacgg tcgaggaaac cggtattgat gaacgccacg cggttgcgcg
     3241 cctgagcaat acagctacgc aagttcagcg aggttcggcg ttcttcatcc ataatgccca
     3301 ttttcagggt attcggtgcc ataccgagca ttgtctcaac gcgggtaaac agtttgttgg
     3361 caaacgccac ttcctgcgga ccgtgcattt tcggtttcac aatatagacg ctgccagtgc
     3421 gcgagttttt ctgcactttt aaatcataaa gggcaatcgc gcctgtcatg acgccatcaa
     3481 gaatgccttc cgggatttca ttgccttcgc tgtcccaaat cacaggaatg gtcatcaaat
     3541 gccccacgtt gcggataaac agcaacgagc gtccgtgcag agaaatttca gagccatcgg
     3601 cggcggtgta atgacgatcg tcattcagtt tacgcacgat ttgccgaccg tttttttcca
     3661 ttttctcttg cagagtcccc tgcatcaggc ccagcagatt gcggtacagc aggattttat
     3721 cttccgcatc aaccgccgcg accgaatctt cgcagtcgag aatggtactg atggcagctt
     3781 cgacgataac atcgttgatg tgcgccggat cgtctttgcc aatccgcccg ttggcatcga
     3841 tttgcagctc aatatgcagg ccgttatttt tcagcaaaat gcaggtcggc gcagcggtat
     3901 cgccacgata accgacaaac tgtgccgggg tacgtaacgt ggtttcttta acatttttca
     3961 actggatgcg taattgtttg tcaaccacct taaacgccac cacatcctga tagctgcctt
     4021 tttccagcgg tagtgattca tcgaggaaac gccgaaccca ggcgataacc tgcgcaccgc
     4081 gctgcggatc gtagccgctg accatcgccc cttcctgcgg gatgatgtcg ctgccgtata
     4141 acgcatcgta cagtgagccc cagcgcgcgt tcgccgcgtt cagcgcgtag cgggcattca
     4201 tcgccggaac caccagttgt ggccccgcct ggctggtgat ttcgctgtca atgcccgtgg
     4261 tttccaccgt cacgcgctcc agttgcggca ccaggtagcc cagttcacgc aggaaagatt
     4321 tataggccgc tttatctttt accggccccg gattgctgcg atgccactca tcaagcgccg
     4381 cctgaatgcg atcgcgttct gccagcaact gacgattttc tggtgccaaa tcgtgaacga
     4441 tttcatcaaa attgcgccag aacgccgcag cgtccagccc tgttcccggt aaaacttctt
     4501 catccacaaa acgtttaaaa ttggcgtcaa tgcgtaaacg gccctgggtt atggtttgac
     4561 tcattgttta tctcctcgtt ttcgcttatt tcgccaacac cgctgctgcc gctttcgcga
     4621 cctgcgcatc ctgtgctccg gttaaaccag aaacgcccac ggcaccaata atttgcccat
     4681 ccacaacaac tggtacgccg ccttccagcg acgtcagtaa cggcgcagtc acgaacgcgg
     4741 tacgtccgtt gttcaccatc tcttcatagc ccttagtttc acgacgcccc agcgcggcgg
     4801 tacgcgcttt ctcctgggag atgtaagccg caatcggcgc gcaatcgtcc atgcgactta
     4861 acgccagcag atgaccgccg tcatcggcaa cagcaatgga aaccgaccag ttatttttct
     4921 gcgcctcttc ctgacctgcg gcaataattg cactcgccat ttgctggcta agaatgactt
     4981 tagttttcat tttgttattc cttttcaagg gcttgttcta caatttcaat ccagtgacgc
     5041 acagaggtac gaccggcgct cgccagatgc gtctggcaac caatgttggc ggtgacgatc
     5101 atttccggtt tgccgctttc cagcgcattc attttgttat cccgcagctg gcgtgccaga
     5161 tcgggatgcg ttaacgcata tgttcccgct gaaccgcagc acagatggct gtcgggaacg
     5221 tccgttaagg taaatccaag acgaagtaac actttttcca cttcgccgtt cagcttttgc
     5281 gcatgttgca gggtacacgg gcagtggaag gccagttttt tatcgccgcg aattgccagt
     5341 ttttccagcg gttcctcgcg cagaagttcg actaaatcga ccgccagttc actgacttga
     5401 cgcgctttat cggcatataa cgcatcgttt ttcagcatct gcccatactc tttgacgaac
     5461 gcgccgcaac cgctggcggt ttgcaaaatt gcctcggcac ctgcttcaat cgcgggccac
     5521 caggcatcaa tattatttcg cgcccgtgcc agccctttct cctgcgcatt aagatgatag
     5581 tccaccgcgc cacaacagcc tgcttcgtta gctgacatga cgctgatccc cagacgatcc
     5641 agcactcgcg cagttgccgc gttggtgttg ggcgaaagcg taggctgggc gcagccttcc
     5701 agcattaaaa cccgacgctt atggcgcagc ggcggacgcg gtttagcttt cacagtttca
     5761 gcaggcagtt ttgctctgac ctgttccggt aaaaacggtc gcagcaccag ccctacctgc
     5821 gtcagcgcac ggaagaccgc cggacgcggc actaactggc gcaatccttc gcgcagtatt
     5881 cgctccggca gtgggcgttt cactttctgc tcgacaatat cacgcccgat atccagcaaa
     5941 ttgtgatagc gcacaccaga aggacaggtg gtttcacaat tgcggcaagt gaggcagcga
     6001 tcgagatgct cctgtgtttt aagcgtgact tcgttgcctt ccagcacctg tttaatcaga
     6061 tagatgcgcc cgcgcggccc gtccagttca tcgcccagaa gctgataggt tgggcaggtt
     6121 gcggtacaaa atccgcagtg aacacaggcg cgcaggatgc tgtcggcttc cagcgcgcgc
     6181 gcgttctgcc gcatctcttc agttaattgg gtttgcatag cctgctcctc agagttccgc
     6241 gtacatgcga ccggggttaa acacgccgca agggtcgagc tgctgtttaa gctgctggtg
     6301 atagcggaat aaaggagccg atagcggggc aaagccacca tctccggcac taaagcgggt
     6361 cgcatgaccg ccagcgttgc gggcgatgcg atggatttga ttgtcctcgg ctgtcgattt
     6421 cagccagcgt aacgcaccgc cccagtcgat cagttgctcg ccgggtaaat ccatcatcgg
     6481 cgcatcgctg ggtaatgaaa tgcgccataa ggtacctggt aacgagaaga acggcagttg
     6541 ttgttcacgc aattgctgcc agaactgacc cgcaacctct tcgcccccca gcagttcacg
     6601 cgccgctttt accgatcctt cgccgccctc aaggcggatc cacaacgcat tgtcgaagta
     6661 acataagcca ctaataggta gcggctggag ttgccactgg gcgatttcgt tcatggcttc
     6721 ttgcaggctg atttcccgac gcaggctcag ggaggcgcgc ggtcgcggta acactttcat
     6781 cgagatttca gtgagcacgc caagacaacc gtagcttccg gccattaacc gtgagagatc
     6841 gtatccggca acgtttttca tcacttcgcc accaaaacgc agatgttttc cagcgccggt
     6901 aatgatgcgc gtgccgagga caaaatcgcg gaccgaaccg ctccacgggc gacgcggccc
     6961 cgccagcccg caggcgacca tcccgcccca ggtggcttct tcaccataat gcggcggctc
     7021 gcagggaagc atttgcccgg cgctctccag cgccgcttca atcgccacca gcggcgttcc
     7081 ggcacgcgcg gttatcacca gctcggtcgg gtcgtaatta acaatgccgc gatgacaacg
     7141 cacatccagc gtttgcccgg tgacagggcg acctaaaaag gctttgctat tgctgccctg
     7201 aatcaccagc ggcgttttat cgctaattgc ctgattcacc tgctccagca gcgcctgact
     7261 gtaatcacac tcgcgtagca tcagaaacgc tccagttcag ggaaaggtaa atgaccgtga
     7321 tgcacatgca tggcaccaaa ttcagcacag cggtgtagcg tgggaatgtt tttcccaggg
     7381 ttcagcaaac catcggggtc aaacgccgcc ttgaccgcat ggaaggtcgt gatttcatcg
     7441 ctgttgaact gggcgcacat ttgattgatt ttttctcgcc cgatgccatg ttccccactg
     7501 atgctgccgc caacttcaac gcagagttcg aggatcttcc cacccagctc ttccgcgcgg
     7561 gcaaattcac cgggttcgtt ggcatcgaaa aggattaacg ggtgcatgtt gccatctccg
     7621 gcatgaaaga cgttggcaac acgtaaatca tattgctgcg ataaacgggc aatgccttcc
     7681 agtacgccag gcagggcgcg acgcgggatg gtgccatcca tgcagtagta atccggggag
     7741 atacgtccta ccgccgggaa cgcatttttg cgaccggccc agaaacgtac gcgctctgct
     7801 tcgtcctgtg ccagacggac gtcagtcgcg cccgctttca acaagatgtc gttaacccgc
     7861 tcgcagtctt cctgtacgtc agactccacg ccgtccagct cgcataacaa aattgcttcg
     7921 gcgtcgacgg gataaccggc atgaataaaa tcttccgccg cgcggatcga caggttatcc
     7981 atcatctcca gcccgccggg gataatgcca ttggcgatga tgtcaccaac tgcaagtccg
     8041 gctttttcta ccgagtcaaa gctggctaac agaacccgcg ccacgggcgg cttcggcagc
     8101 agttttaccg tcacttcggt ggtcacgccg agcatacctt ccgatccggt gaacagcgcc
     8161 agcaggtcaa aaccaggtga atccagcgcg tccgatccca gcgtcagcgc ctcgccgtcc
     8221 agcgtttgca cttcaatttt cagcaggtta tgtacggtca gaccatattt caggcagtgg
     8281 acgccgccgg cattttctgc cacgttgccg ccaatggaac aggcgatttg tgaggaaggg
     8341 tccggtgcgt agtagagatt atgcggtgca acggcctggg agatcgccag gttacgcacg
     8401 cctggctgca cgcgcgcgcg gcgaccaacg gggttaatgt cgaggatctc tttaaagcgc
     8461 gccatcacca acaacacacc tttttccagc ggcagcgcgc caccagaaag cccggtgcct
     8521 gcaccacggg tcaccaccgg tacacgcagg cgatggcaga cagccagaat cgctgtcacc
     8581 tgttccattt gcttaggcag aacaaccagt aatggacgcg tgcgatacgc gctcaacccg
     8641 tcacactcgt aaggaatgat ctcctcatcg gtatgcagga tctcaagtcc agggacatgc
     8701 tcacgcagtg ccatcagtac cgatgtgcgg tcgacatcgg gtaaagcgcc atcaagacgc
     8761 tcttcgtaca agatgctcat gagtaggctt cgctttgttg tgtggcagct gatttttgcg
     8821 cgctgcttct gtgaacagtt attaagcggg cttttcgttt tcgtctatct ctttagctac
     8881 cggtcagacc attttttttc cagctctgtg accttgcctt ggttaactca atgttaaatt
     8941 gatgtaacat aatcacttac gtgatgtgcg tgttttgcga gttaagaaca gaaaaattgg
     9001 tcctacctgt gcacgaggtc cgggaatgaa agatgaacgt cgccctattt gcgaagtggt
     9061 tgcagagagt atcgaacggt taattatcga cggcgtactg aaggtcggtc agccacttcc
     9121 ctcggaacgt cgactgtgtg aaaagctcgg cttctcacgc tccgcactgc gtgaagggct
     9181 gaccgtgctg cgcgggcgcg ggattattga aacggcgcag ggtcgcgatt ctcgtgtcgc
     9241 acggcttaat cgggtgcagg acaccagccc gctgatccat ctgttcagta cgcagccgcg
     9301 aacgctgtac gatctgctcg acgttcgcgc attactggag ggcgaatcgg caaggctggc
     9361 ggcaacgctg ggaacgcagg ctgattttgt tgtgataacc cgctgttatg aaaaaatgct
     9421 cgccgccagt gagaacaaca aagagatttc gctgatcgaa catgcgcagt tggatcacgc
     9481 tttccatctc gccatttgtc aggcttctca caatcaggtg ctggtgttta cgctgcaatc
     9541 attgaccgat ctgatgttta attcagtgtt tgccagcgta aataatctct accatcgacc
     9601 acagcaaaaa aagcagatcg atcgccagca tgcgaggatc tacaacgcgg tgttgcagcg
     9661 gctgccgcac gtcgctcagc gcgcagcacg cgatcatgtg cggaccgtga aaaagaatct
     9721 ccacgatatc gagctggaag gccaccattt gattcgctcg gcggtgccgc tggagatgaa
     9781 cctgagttag ctggtattaa atctgctttt catacaatcg gtaacgcttg tacggctccg
     9841 ccccaatgcg ttccagcatg ttattcatgc ctgtattggt ttcgaggatc catgacatct
     9901 ccagcgcatc gatcttccgg cgggcaaacg gatcgcgtaa ggcttcaatc aataacagcg
     9961 caatcaccgg gccgatgcgg ctgaactgat actcgtcgcg cacgcccatc agcggtactc
    10021 gcgcagttcg cacaccgctg actttcaaac gccacagcaa ttttgcccag ccgaagggaa
    10081 agagcgatcc gttcagatcg gcaatcgcct cgttgatgtt cggcaagccg acaataaacg
    10141 cgcagggtgc agaatcaatc tcagcgatgt agatcatatc gtccggcacc agatatttaa
    10201 gttgatcgcc catggtcgcg aattcatgtt cggtaaacgg cacaaatccc cagttgtgct
    10261 gccagccaga gttgaaaatc tcacgcagga tctgcatctc ttcggcaaac cgctgacgat
    10321 tgatgcagcg aatggtcacc tttttgcgca cctgatccat cagttttttt agcgccggag
    10381 agaaagtgag atcggttcgc tgcatccacc acgccagtaa atcaatgcct ttgtgataac
    10441 ccagttgttc aatatgcgcg gcataccacg gtttgccgtg tggcatcatc gcacagggtg
    10501 gtgtgtcaaa accttcaatc agtaatccgc tttcctgatt gatattcagg ctgaaaggcc
    10561 cgctgatctt acttgcacct tgtgacttca accacgcttc cgctgcgcca aacaacgcgg
    10621 caaaaacctg cggatcatca atggcgtcaa tcatgccgaa atgaccggta tcttcaccgt
    10681 aacgctcaca gtgcaaggta tctatttgcg cggtaatacg cccaactatc tgccccgctt
    10741 tttttgccac ccacgcctgc cagatgatat ggtccgtccc cggatttttc gcagacaaat
    10801 gctcgttgcg ttcaatgaat aaagggggta tccagtttgg atcgtcggga taaagtgatg
    10861 acggaaaagc gataaatgcc ttaaggtcat ttttattaat tactttttca attttaatca
    10921 tcagcagatc gcattccata tctctgtgga taacaaataa ttaacggtga aaaacaggtt
    10981 gcaacagcaa cgaacgacac cttaataatc aacccaatga atacaaacaa agaataaata
    11041 gtatttatta atga
//
LOCUS       DAJOWB010000137        11043 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJOWB010000137 DAJOWB010000000
VERSION     DAJOWB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.137"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            166..927
                     /gene="adiY"
                     /locus_tag="OHO85_004022"
     CDS             166..927
                     /gene="adiY"
                     /locus_tag="OHO85_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCQ2673324.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            1064..2401
                     /gene="adiC"
                     /locus_tag="OHO85_004023"
     CDS             1064..2401
                     /gene="adiC"
                     /locus_tag="OHO85_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCQ2673325.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2505..4148
                     /gene="eptA"
                     /locus_tag="OHO85_004024"
     CDS             2505..4148
                     /gene="eptA"
                     /locus_tag="OHO85_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCQ2673326.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4145..4813
                     /gene="basR"
                     /locus_tag="OHO85_004025"
     CDS             4145..4813
                     /gene="basR"
                     /locus_tag="OHO85_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCQ2673327.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4814..5914
                     /gene="pmrB"
                     /locus_tag="OHO85_004026"
     CDS             4814..5914
                     /gene="pmrB"
                     /locus_tag="OHO85_004026"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCQ2673328.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5890..5979)
                     /gene="pmrR"
                     /locus_tag="OHO85_004027"
     CDS             complement(5890..5979)
                     /gene="pmrR"
                     /locus_tag="OHO85_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCQ2673329.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6091..7593)
                     /gene="proP"
                     /locus_tag="OHO85_004028"
     CDS             complement(6091..7593)
                     /gene="proP"
                     /locus_tag="OHO85_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCQ2673330.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7857..8735)
                     /locus_tag="OHO85_004029"
     CDS             complement(7857..8735)
                     /locus_tag="OHO85_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCQ2673331.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGNLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            complement(8732..10960)
                     /gene="crfC"
                     /locus_tag="OHO85_004030"
     CDS             complement(8732..10960)
                     /gene="crfC"
                     /locus_tag="OHO85_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCQ2673332.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDRLIQKLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHDDLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQARGYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEVKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2781 a         2864 c         2700 g         2698 t
ORIGIN      
        1 tgtaaaaaaa tatgtacatg agaaattact ataaaaattt gtaatattag taaaactcgt
       61 tatttttatg catgtttata ttcatcatac aattatataa ccatttcccg gtatcgcttt
      121 gctttagcga gaactggtct tttatttgca ctcaggtgta catgtatgag gatttgcagc
      181 gaccaacctt gtattgtttt attgactgaa aaagatgtct ggataagggt gaatgggaaa
      241 gaacctatta gccttaaagc taaccatatg gcgttattaa attgtgaaaa taatattatc
      301 gacgtctcct ctcttaacaa cactttggtt gctcatatta gtcacgacat catcaaagat
      361 tacctccggt ttctgaataa agatctctcg caaataccag tatggcaacg tagcgctacg
      421 cccatactca ccctgccatg cctgacgcca gacgtctttc gcgttgccgc gcaacacagc
      481 atgatgcccg cagaaactga gtcagaaaag gaacgaacac gcgcattatt attcacggtg
      541 ctatcccgtt ttctcgacag taaaaaattc ctttcattaa tgatgtatat gttacgtaat
      601 tgtgtaagtg acagcgttta tcaaattatt gaaagcgata ttcacaaaga ctggaatctt
      661 agtatggtag ccagttgttt atgtcttagc ccaagtctgt taaagaaaaa gctgaaaagc
      721 gaaaacacca gttatagcca aataatcacc acctgccgca tgcgttatgc cgtaaatgaa
      781 ttaatgatgg acggtaaaaa tatctctcag gtatcacagt cctgcggcta caacagtacg
      841 tcgtacttta tttctgtctt taaagacttc tacggtatga cgccgctgca ttatgttagt
      901 cagcacagag aacgcactgt cgcctgattt ttaaccttaa cgaagagcta tattaataac
      961 ggcatcagcg ataacccggt cgataataat tcaactatcg aatgcaggcg tatgatatga
     1021 cgtaatttat tgtcacgaag ctcgccttcg caggagttta attatgtctt cggatgctga
     1081 tgctcacaaa gtgggcttaa tccccgtcac cctgatggtg tcggggaata ttatggggtc
     1141 aggtgttttt ctgttacctg caaacctggc ctcgactggc gggattgcca tttatggatg
     1201 gttggtgacg attatcggtg cgctggggct ctcgatggta tacgccaaaa tgtcgttcct
     1261 cgatccaagt cctggtggtt cttacgctta cgcccgccgc tgctttggcc cgtttctcgg
     1321 ttatcaaacc aacgtcctct actggctggc ctgctggatc ggcaatatcg ccatggtggt
     1381 cattggcgta ggatatttaa gttacttctt cccgattctg aaagatccat tggtattaac
     1441 catcacctgc gtcgtggtgc tgtggatctt cgtcctgctg aacattgtcg gtccgaaaat
     1501 gatcacccgt gtgcaggcag ttgccaccgt gctggcgctg atccccatcg tcgggattgc
     1561 cgtatttggc tggttctggt tccgtggtga aacctatatg gcggcatgga acgtcagcgg
     1621 cctgggcacc ttcggtgcaa ttcaaagtac ccttaacgtt acgctgtggt cgttcatcgg
     1681 tgtggaaagt gcctccgttg ccgcaggtgt ggtgaaaaac ccgaaacgca atgtccctat
     1741 cgccaccatt ggtggggtat tgattgccgc cgtttgctat gtactttcta ccaccgcgat
     1801 tatggggatg atccctaatg ccgcactgcg cgtttctgct tcgccattcg gtgatgccgc
     1861 acggatggcg ttgggtgaca ccgccggggc cattgtttcc ttctgcgcag ctgcgggttg
     1921 cttaggttca ctgggcggct ggacgttgct ggcgggtcaa acggcgaaag ccgctgccga
     1981 tgacggactg ttcccaccaa tttttgcccg tgtaaataaa gcgggtacgc cagtggcggg
     2041 gctgattatc gtcggtattt tgatgaccat cttccagctc agcagcattt caccaaacgc
     2101 gaccaaagag ttcggtctgg tttcttccgt ctcggtcatc tttacactgg tgccatatct
     2161 ttacacctgt gcggcgttac tgctgctcgg acacggtcac tttggtaaag cacgcccggc
     2221 atatctggca gttactacca ttgccttcct ctactgcatc tgggccgtgg tggggtccgg
     2281 agcgaaagag gttatgtggt catttgtcac cctgatggtc atcaccgcca tgtatgcgct
     2341 gaattacaac cggctacata aaaacccgta tcccttagat gcaccaataa gcaaagatta
     2401 attcccctta atccagcaaa cataaaagcc aaccttaaga acttaaggtt ggcttaattt
     2461 tgctttgcga gcatatgcgc actttgttcg atggaaacac cgtgatgttg aagcgcctac
     2521 taaaaagacc ctctttgaat ttactcgcct ggctattgtt ggccgctttt tatatctcta
     2581 tctgcctgaa tattgccttt tttaaacagg tgttgcaggc gctgccgctg gattcgctgc
     2641 ataacgtact ggttttcttg tcgatgccgg tcgtcgcttt cagcgtgatt aatattgtcc
     2701 tgacactaag ctctttctta tggcttaatc gaccactggc ctgcctgttt attctggttg
     2761 gcgcggctgc acaatatttc ataatgactt acggcatcgt catcgaccgc tcgatgattg
     2821 ccaatattat tgataccact ccggcagaaa gttatgcgct gatgacaccg caaatgttat
     2881 taacgctggg attcagcggc gtgcttgctg cgctgattgc ctgctggata aaaatcaaac
     2941 ctgccacctc gcgtctgcgc agtgttcttt tccgtggagc caatattctg gtttctgtac
     3001 tactgatttt gctggtcgcc gcactgtttt ataaagacta cgcctcgttg ttccgcaata
     3061 acaaagagct ggtgaaatcc ttaagcccct ctaacagcat tgttgccagc tggtcatggt
     3121 actcccatca gcgactggca aatctaccgc tggtgcgaat tggtgaagac gcccaccgca
     3181 acccgttaat gcagaacgaa aaacgtaaaa atttgaccat cctgattgtc ggcgaaacct
     3241 cgcgggcgga gaacttctcc ctcaacggct acccgcgtga aactaacccg cggctggcga
     3301 aagataacgt ggtctatttc cctaataccg catcttgcgg cacggcaacg gcagtttcag
     3361 taccgtgcat gttctcggat atgccgcgtg agcattacaa agaagagctg gcacagcacc
     3421 aggaaggcgt gctggatatc attcagcgag cgggcatcaa cgtgctgtgg aatgacaacg
     3481 atggcggctg taaaggtgcc tgcgaccgcg tgcctcacca gaacgtcacc gcgctgaatc
     3541 tgcctggtca gtgcatcaac ggcgaatgct atgacgaagt gctgttccac gggctggaag
     3601 agtacatcaa taacctgcag ggtgatggcg tgattgtctt acacaccatc ggcagccacg
     3661 gtccgaccta ttacaaccgc tatccgccgc agttcaggaa atttacccca acctgcgaca
     3721 ccaatgagat ccagacctgt agcaaagagc aactggtgaa cacttacgac aacacgctgg
     3781 tttacgtcga ctatattgtt gataaagcga ttaatctgct gaaagaacat caggataaat
     3841 ttaccaccag cctggtttat ctttctgacc acggtgaatc gttaggtgaa aatggcatct
     3901 atctgcacgg tctgccttat gccatcgccc cggatagcca aaaacaggtg ccaatgctgc
     3961 tgtggctgtc ggaggattat caaaaacggt atcaggttga ccagaactgc ctgcaaaaac
     4021 aggcgcaaac gcaacactat tcacaagaca atttattctc cacgctattg ggattaactg
     4081 gcgttgagac gaagtattac caggctgcgg atgatattct gcaaacttgc aggagagtga
     4141 gtgaatgaaa attctgattg ttgaagacga tacgctgtta ttgcagggat tgattctggc
     4201 ggcgcaaacc gaaggctacg cgtgcgatgg cgtgacaacc gcgcggatgg cggaacaaag
     4261 ccttgaggcc ggtcattaca gcctggtggt actggattta gggttacccg acgaagatgg
     4321 actgcatttt ctcgcccgta tccggcagaa aaaatacacc ctgccagtac tgatcctcac
     4381 cgctcgcgat acgctgaccg acaaaatcgc cgggctggat gtcggtgccg atgactatct
     4441 ggtgaagcct tttgcgctgg aagagttaca cgcccgtatc cgcgccctgc tacgacgcca
     4501 taataatcag ggcgaaagtg agctgattgt tggcaatctg acgctgaata tgggtcgccg
     4561 tcaggtatgg atgggcggtg aagagttgat tctgacgccc aaagaatatg ctctgctgtc
     4621 acggttaatg ctcaaagcag gcagtccggt acatcgggaa attctctaca acgacatcta
     4681 taactgggac aatgaaccct cgaccaacac cctggaagtg catatccaca atctgcgcga
     4741 caaagtgggc aaagcccgta tccgcaccgt gcgcggcttt ggctatatgc tggtcgcgaa
     4801 tgaggaaaac taattgaatc tgatgcattt tctgcgccga ccaatatcgc tgcgccaacg
     4861 gctgatattg accatcgggg ccattttgtt ggtgtttgag ctgatcagcg tcttctggct
     4921 atggcatgaa agtaccgagc agattcagct gtttgagcag gcgctacgcg acaatcgcaa
     4981 caacgatcgc cacatcatgc gtgagatccg cgaggcggtc gccagcctga ttgtccccgg
     5041 cgtctttatg gtcagcctga cgctatttat ctgctatcag gcggtgcgcc gcatcacccg
     5101 cccgctggcg gagctgcaaa aagagctgga agcgcgcact gccgacaact taacgcccat
     5161 tgccattcac agcgccaccc tcgaaatcga agcggtggtt tcggcgttaa acgatctggt
     5221 cagtcgcctg accagcacgc tggataacga aaggttgttt accgctgacg tcgcgcacga
     5281 actgcgaacg ccactggcgg gggtgcgttt gcatctggaa ctgctggcga aaacgcatca
     5341 cattgatgta gcaccgttag tggcacgact tgatcagatg atggagagcg tctcccagct
     5401 gctgcaactg gcgcgtgccg gacagtcatt ttcttccggt aattatcaac atgtaaaact
     5461 gctggaagat gtcattctcc cctcgtatga cgaactcagt accatgctcg accagcgaca
     5521 gcaaaccctg ctattgccag agagcgccgc cgacatcacc gttcagggtg atgcaaccct
     5581 gctgcggatg ttattgcgaa acctggtaga aaacgcccat cgttacagcc cgcaaggcag
     5641 caacattatg attaagctgc aagaagatgg cggagcggtc atggcagttg aagatgaagg
     5701 accaggtatt gatgagagta aatgcgggga gttgagtaaa gcgtttgtac gtatggacag
     5761 ccgttatggc gggattggtc tggggttaag tattgtcagc cgcattacac agttgcatca
     5821 cgggcagttt ttcctgcaaa accggcaaga gacttccggc acgcgggcct gggtacggct
     5881 gaagaaagat cagaacgtgg caaaccagat ataaagaaag ctgctgacca ccagcacgct
     5941 gaacacggta gttaaacttt cataaacacg gtttttcatt gcactctcct ccgttaacct
     6001 ggaggagagt atgcgcgtca gagattaagt gaaccttaag agttcaattg gcctgataag
     6061 acagcgtcac atcaggccat ccgtttcagc ttattcatca attcgcggat gttgctgcac
     6121 caggcgggta cgtttcgcct gcaaatcggc aatctcgtgg tcaatattat cgattttctg
     6181 ctcgatatta tcgtaatgct cgacgagaat ttccttcgct tcctgtatat ctgacgccgc
     6241 cggtgtcgca cctttcaacg gacgatttgc cgtctctttc atggttacgc cggtgattaa
     6301 accaatcacc gccactacca tcaggtaata ggcaggcatc atcagattct gcgagctttc
     6361 gaccagccag gccgccagtg ttggcgtcag accggcaacc agcaccgaaa tattaaatgc
     6421 cgccgccagc gcgctgtaac ggatatgcgt cgggaacatc gctggcaagg tagaagccat
     6481 aacgcccgta aagcagttaa ggatcaccgc cagcatcagt aacccggcaa aaatcaggcc
     6541 gatgacgtta ctgttaatca gaataaacgc cgggatcgcc aacacaaaca gggcaacact
     6601 accaagtagc acaaacggac gacggccaaa acggtcactc agcaagccca tcaccggctg
     6661 gacaaacagc ataccgatca taatggcgat aataatcagc accccgtggt cttcggagta
     6721 atgcaggtta tgcgacaaat aactcggcat ataggtcagc aacatgtagt aagtcacgtt
     6781 ggtggcaatt accagaccaa tacatgtcaa caggctgcgc cagtgtttgg tggcaatctc
     6841 tttaaacgag actttcgggc catcctgcaa accttcgcgg tcgccctgtt ccagtttatc
     6901 gacatgctgc tggaacgccg gagtttcttc cagcgcatgg cgcaggtaaa gcccgataat
     6961 ccctaacggc agagcaataa agaacggaat acgccagccc cagtcgagga agttcgcttc
     7021 gccgacaatg gtcgaaatta acaccactac gccagcaccc agcacaaacc cggcaataga
     7081 accaaagtcc agccagctgc ccataaagcc acgtttacgg tcaggggagt attccgcaac
     7141 aaatatcgac gccccggtat attcaccgcc gaccgagaaa ccttgtgcca tcttacagat
     7201 caacagcaga atcggtgccc aaataccaat cgtgtcgtag gacggtatta agccaataca
     7261 gaacgtactg atcgacataa tcacaatagt gatagcgagg atcttctggc gaccatattt
     7321 atcgcccaac ataccaaaga agagtccgcc aagcggtcga atcagaaagg gaacggagaa
     7381 cgtggcaagt gcagcaacca tctgcacgct ggggtcagcc cccgggaaaa aaactttacc
     7441 taatgcgtaa gcaacaaaac cataaacacc aaaatcaaac cattccattg cattacccag
     7501 tgatgctgcg gtaatggctt tacgcagttt accgtcatca ataatggtga catcacgaag
     7561 ggtaatcggt tttacttttt tccttttcag catagctttc ctcgcagagt tggcccattt
     7621 ccgcaaacgc cagaaaaatc agcgcgacgg ccgcaaaagg ggagcgggaa ttgcaggatg
     7681 caatctctgt aacaagcgta gcgggtttgc ttacaccctc cggttaagga atgtacaatc
     7741 aaaccagttg acgcctgaat tgggcagtta atgacctttt taccctacca gtgtttaaat
     7801 ttgtgatcaa cttcacacat ggatttcgtt taggactcat tgatgtaact gattatttac
     7861 gccaaattta cagagaagat ttcctcgacc attgcccccg ccagacgctg cgctgaacag
     7921 agtcgtggca tccccagcgc gacagtttgc cagcgttggg tcactgacca gcaaacgggt
     7981 tgtttatctg ccgtggtcca ctttcccagc caacttaaaa aatcagcatg atccccgccc
     8041 gtcggaacgg tcgaagaggc aaaccactga tggtaatagt gacgcgtggt cggggccgca
     8101 ttgatactct ctgccagatg ttgtgtcgct ttcacgcgca aattacccag caacaacgcc
     8161 accgcttgcg ggcttcccag acgacaggca tcgccaaatg ccccccaacg caactcttcg
     8221 agaacgataa aacagcgccc aggcaacgac caggcgttat acgccccagc acgccagcgg
     8281 gtaaaaattt gctcgctctg ttcacccgcc tgcaccagta ggcctcgttg cgttaatgcc
     8341 ttttccttcc agcgacaacg ggctgaaaac cactcataca aggcggcaat ttgctggggg
     8401 agctccggga aagcagagtg ctggcggtga tccatttttt gcagcagcgc cagatgctgc
     8461 gcgatctgcg tcagtgccag atgaccggga tccagcatcg acgccagttt ttccaccaat
     8521 gtcagacgca ggctaagcgg cgcatttaac atgccgccgt tcgtccatgt ctgaagctgc
     8581 acgcgcgcaa gaagttcctg catcagtcgt tcacgaaact gctgctgatg ggcttgtgga
     8641 aggcgggcat catcgccctg cgccagatcc actaaaaagc ggggataaac cgactccagc
     8701 acgcgaccgg ggccgtcaag taacgtcttg gtcaatatcg ttctgccgtg aaaagtgttt
     8761 gaatatcatc gcgtaacagc tgggcgtcgg tgtaaatcca gccgtgagtc atcacagtct
     8821 gctgcaattg ctgctgcatc agcctgacca ccgattcatt ttgttgacgc agagccagac
     8881 tttcgcgtaa acgcgtctgt aattccgtca aacatgaagc gaactcagcg aaaaaagtat
     8941 tcatgcctgc cgtaacagat tcatcgacct gctctgccag aactttagcc atttgttggc
     9001 aataaagatc gacttctgcg cttaaagctc gttgcaacac actgtaatca accgtttctg
     9061 tcggggattt ctcatttccc cgtccccagt cgggctgatt caaccagcgc gaaaaagtct
     9121 cacgcacaac gcctaaacgc gtgctctgct cgtccgttgc ttcctggcgc gaaatgactt
     9181 cactgaacag ctggcgagtg ttgaagtggg gaactacgcc gtgaaaaaca ggaaaatgaa
     9241 acccaggacg aaaccctgac tcgctcaatt ccattttgac ttgttgctca atgggccgaa
     9301 taacatcggt taacacccgg caaagggtgg attcaagctc ggcaaaacgc agcgtaaagt
     9361 cgcgactgat ggtgttctgc gccgtctgta acagtgtctc acagcaggta cgaatctcgc
     9421 ttaacggctc cgaatcatcc tgaaacaagg cggctaactg cgcattcagc gcatcttgct
     9481 gttgacgcag aaagtggttg gcggaggtca gggccagctc gatttcatgt ttaacctcgc
     9541 cgctcacctg cgcctgattg agttgcaata gctgcaaact ttcttcgacc tgatggatat
     9601 tttgccgcaa ttgttcacaa gcgacgttta acccgtgcgc acgaaaatcc aggtatcccc
     9661 gcgcctgctg cgcgtaattc aacagtttat gcgcagcaga tcgcagagca tacaacgagg
     9721 cgttagcgta agcggcatga agcaacgcct gaattggctg ggcgaacagc gaatcttccc
     9781 acaactgatc ggcagcatga cgaatatgtt cgaggtccgc cagatcgtca tgacgccagc
     9841 gcctgccgag cgcagcatgg gcaaaatctt ccacccagcg ttgttgctct ggcgctggta
     9901 acttaccgtt gttggctaac tcatggcgcg ctcgattcgc caggtagccc cacatcgacg
     9961 acaccggaaa tatctgctgt ggcgtaatac agcctttcat cagcgtcccg gaaatcagtg
    10021 cccgcacctg gtcggcgtcg tcactgttac gatcctgttg atcgaactta ttgaccagca
    10081 catacagcgg caccgattgc cccaccgcca aaatcgcctc acggacctct tcatcggaga
    10141 tcgatttcag ttgcgtataa tccagcaccg ccagtaccgc cgaggcgcgt gccagctgct
    10201 ggttaagcat tttttgcaga tgcggttgcc cggcttcatt tggcccgggg gtatccagta
    10261 acgtcaactg accgggataa ctctccagcc ccgccagatg gacaaactcc acttcaatca
    10321 cgggaatatg ctcaatggcg gcgtaagcag aaaaaggaaa atcgacgtcc agcgccttcg
    10381 ccagtcgcac taaatcattc aaacttttca gacaatgaaa aataggctgg gcacccagat
    10441 aatatttttc gaaagcgacg ccattttcga tccgctgcat aagcgcacgc atatctttat
    10501 ctatttccag cacatcagtc agatgcttaa tatcgcaatc acgcaggcgc tgttgtaatt
    10561 tttgaattaa acggtcgatt ggcgcgacat gtgaaaaatg cagtaccggt tctttttgcc
    10621 cgggcgtatg gcgaataagc gtcggcagcg cagtcattgg gcgattacga ttaggcaaaa
    10681 cctccgtacc aacaatggca ttaatggtgg ttgatttccc tgctttcatg gtaccgacaa
    10741 tcgccagcac catttccagt cgggaaattt tacgcaactc attattcagc atcgcgtgac
    10801 gttcggcgat attaggctga ctccagggta aagccagttg tggcgcatcg tctccgggta
    10861 cagagagagg cattttttcc agtaactgca actgttggcg agaaagctgt aacaggcgtt
    10921 cagcctcctg acttaactca tacagggtct gtgtgtacat agaaaattct tccttaaagc
    10981 aaattttgtt acttttattt agccagattg tttttgagtt ctgttttcgg cttttataat
    11041 tac
//
LOCUS       DAJOWB010000138        10969 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJOWB010000138 DAJOWB010000000
VERSION     DAJOWB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.138"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..462
                     /locus_tag="OHO85_004031"
     CDS             <1..462
                     /locus_tag="OHO85_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HCQ2673333.1"
                     /translation="SEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSI
                     IAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRG
                     NAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGFS"
     gene            731..2968
                     /gene="nrfB"
                     /locus_tag="OHO85_004032"
     CDS             731..2968
                     /gene="nrfB"
                     /locus_tag="OHO85_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCQ2673334.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPNAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2955..5927
                     /gene="nfrA"
                     /locus_tag="OHO85_004033"
     CDS             2955..5927
                     /gene="nfrA"
                     /locus_tag="OHO85_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HCQ2673335.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5928..6818
                     /locus_tag="OHO85_004034"
     CDS             5928..6818
                     /locus_tag="OHO85_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCQ2673336.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7001..7762
                     /gene="envY"
                     /locus_tag="OHO85_004035"
     CDS             7001..7762
                     /gene="envY"
                     /locus_tag="OHO85_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCQ2673337.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            8275..9228
                     /gene="ompT"
                     /locus_tag="OHO85_004036"
     CDS             8275..9228
                     /gene="ompT"
                     /locus_tag="OHO85_004036"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HCQ2673338.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            9415..10899
                     /locus_tag="OHO85_004037"
     CDS             9415..10899
                     /locus_tag="OHO85_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCQ2673339.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
BASE COUNT         2968 a         2705 c         2651 g         2645 t
ORIGIN      
        1 agtgaaaaga gtcacggcag agaagaaatc cgtcttcata ttgtttgcga tgtccctgat
       61 gaacttattg atttcacgtt tgaatggaaa ggtctgaaga aattatgcgt ggcagtctcc
      121 tttcggtcaa taatagcaga acaaaagaaa gagcccgaaa tgacggtcag atattatatc
      181 agttctgctg atttaaccgc agagaagttc gccacagcaa tccgaaacca ctggcacgtg
      241 gagaataagc tgcactggcg tctggacgtg gtaatgaatg aagacgactg caaaataaga
      301 agaggaaacg ccgcagaatt attttcaggg atacggcaca tcgctattaa tattttaacg
      361 aatgataagg tattcaaggc agggttaaga cgtaagatgc gaaaagcagc catggacaga
      421 aactatctcg cgtcagtcct tgcggggagc gggttttcgt aatcttgccc tgcctgctca
      481 cctcctccag gcacttccac tacattatga cctgctcggt gccaccgctc cttttcacta
      541 tgacgttaac ctcccctcct tgacaccatg cttttgtcta cattctacgc aaacggcaac
      601 ttttataacc agtgtaaaaa taacgtgccg caatagtcat acctattaat ggtaaaaagc
      661 tgtcacaatt cataaaaaac cttaatatac gccaccctaa acataaccag cgttaatgta
      721 aggtttttgt gtggactggc ttcttgatgt ttttgcaacc tggctctacg gcttaaaagt
      781 aatcgcgata acgttagcgg tcatcatgtt catcagcggg ctggacgatt tttttattga
      841 tgtcgtctac tgggtacgcc gcattaaacg caagttgagt gtttatcgcc gctacccgcg
      901 aatgagttac cgcgaactgt ataaaccaga tgaaaaaccg ttagcgatta tggttccggc
      961 gtggaatgaa acgggcgtca tcggcaatat ggccgagctg gcggcgacca cgctcgacta
     1021 cgaaaactat catatctttg ttggcaccta ccccaacgac cccgatactc aacgtgatgt
     1081 tgacgaagtg tgcgctcgct ttccgaacgt gcataaggta gtctgcgcgc gtcctggtcc
     1141 caccagcaaa gccgactgtc tgaacaacgt gctggacgcc atcacccagt ttgaacgtag
     1201 cgccaatttc gcttttgctg gttttattct gcatgacgcc gaagatgtga tttcaccgat
     1261 ggaattgcgt ctgttcaact atctggtcga gcgtaaagat ctgattcaga tcccggtgta
     1321 tccgttcgaa cgcgaatgga cgcacttcac cagcatgact tacattgatg agttttcaga
     1381 gctgcatggc aaagatgttc cggtgcgtga agccctcgcc ggacaagtgc ccagcgcagg
     1441 cgtcggcacc tgtttcagcc gccgcgccgt gaccgctctg ttagctgacg gtgacggtat
     1501 tgctttcgac gtgcagagtc tgactgaaga ttacgacatt ggtttccgcc tgaaagagaa
     1561 aggtatgacg gaaatttttg tccgttttcc ggtggtggac gaagccaaag aacgcgagca
     1621 gcgcaaattt ttacagcacg cacggacgtc aaacatgatc tgcgtgcgcg aatatttccc
     1681 cgataccttt tcgactgcag ttcgacaaaa atctcgctgg atcatcggca ttgttttcca
     1741 gggctttaaa acccacaaat ggacctccag cctgacgctg aactactttc tctggcgcga
     1801 ccgcaaaggg gcaatcagta actttgtcag cttcctcgcg atgctggtga tgctccagct
     1861 tttgctgttg ctggcgtatg aaagtttgtg gcccaatgcc tggcatttcc tttctatttt
     1921 tagcggcagc gcatggttaa tgaccctgct gtggctaaac tttggcttga tggttaaccg
     1981 catcgtgcag cgggtgattt tcgtcactgg ctactacggt ctgacgcagg ggctgctatc
     2041 tgtcctgcgt cttttctggg gcaacctgat taacttcatg gccaactggc gcgcgctaaa
     2101 acaggtactt caacacggcg atccacgtcg cgtcgcgtgg gataaaacaa cgcatgactt
     2161 ccccagcgtg actggcgata cccgctcgtt gcgcccgtta ggccaaattc tgctggaaaa
     2221 tcaggtcatc actgaagaac aactcgatac agcactgcgt aatcgcgtcg aaggtctacg
     2281 cctgggcggt tcaatgctga tgcaggggct gattagcgcc gagcagctgg cacaggcgct
     2341 ggcagagcaa aacggcgtgg cgtgggaatc catcgatgcc tggcagatcc cgtcctcgct
     2401 gattgccgaa atgccagcct ccgtagcgct gcattatgca gtactgccgc tgcgtctgga
     2461 aaatgatgag ttaattgtcg gcagtgaaga tggtattgac ccggtttcgc tggcggccct
     2521 gacgcgtaaa gtcggacgca aagtgcgtta cgtcattgtt ctgcggggac aaattgtcac
     2581 ggggttacgt cactggtatg cacgccgacg cggtcacgat ccgcgggcaa tgttgtacaa
     2641 tgcggttcag catcagtggc tcacggaaca gcagaccggt gaaatctggc ggcaatatgt
     2701 gccgcatcag ttcctgttcg ccgaaatact gaccacgctc ggtcatatta atcgttcagc
     2761 aattaacgtg ttgttattgc gccatgaacg cagttctctg ccgctcggca agtttttggt
     2821 caccgaaggc gttatcagcc aggaaacgtt ggatcgcgtc ctgacaattc aacgcgaatt
     2881 acaagtttcg atgcaatcac tattactcaa agcaggttta aacacagaac aggttgcgca
     2941 actggagtcc gaaaatgaag gagaataacc ttaatcgcgt catcggatgg tctggtttac
     3001 tgctgacgtc tttattgagt accagcgcac tcgcagacaa tattggcacc agcgcagaag
     3061 agctggggct gagcgattat cgccattttg ttatttatcc ccgtctcgat aaggcgctga
     3121 aggcacagaa aaataacgac gaagcaaccg ccatccgcga atttgaatat atacaccagc
     3181 aggtgccgga taatattccg ctgactttat accttgcgga agcctatcgc cattttggtc
     3241 atgatgaccg ggcgcggctg ttgcttgagg atcaactgaa acgtcaccca ggagatgccc
     3301 gacttgagcg cagtctggcg gctattccgg ttgaagtgaa aagcgttacg actgttgaag
     3361 aactgcttgc ccagcaaaaa gcgtgcgacg ctgcgccgac cctgcgttgt cgcagtgaag
     3421 tcgggcagaa tgccctgcgg ctggcacaat tacctgtcgc cagagcgcaa ctgaacgatg
     3481 cgacgtttgc tgcatcgcca gaaggaaaaa cgctgcgaac cgatctgctg caacgggcaa
     3541 tctacctgaa acaatggtcc caggcagata cgctatacaa tgaagcacgc cagcagaaca
     3601 cattaagcgc ggcagaacgc cgtcagtggt ttgacgtgct tcttgccggg cagctggacg
     3661 atcggatcct ggcactgcaa tcacagggga tcttcaccga tcctcagtca tatattactt
     3721 acgcgaccgc gctggcttat cgtggcgaaa aagcacgcct ccagcattat ctcaatgaaa
     3781 ataagccgct gtttaccacg gacgcacaag agaaaagttg gctctatctg ttatctaaat
     3841 acagcgccaa ccccgttcag gcattggcga attatacggt gcagtttgcc gataaccgcc
     3901 agtatgttgt tggcgcgacg ctaccggtgc tgttaaaaga aggtcagtac gacgcagcgc
     3961 aaaaactgct cgccaccctc cccgccaatg aaatgcttga ggagcgttat gctgtcagcg
     4021 tggcgacccg taacaaggct gaatctctgc gtctggcacg attgctgtat cagcaagaac
     4081 cggcaaatct tacccgcctg gatcaactga cctggcaact gatgcagaac gagcagtcac
     4141 gcgaagctgc cgatttattg ctgcaacgct atcctttcca gggcgatgcg cgtgtcagcc
     4201 agactttaat ggcgcgactg gcgtctctgc tggaaagtca tccttacctg gcaacgccgg
     4261 cgaaggtggc gattttatcg aaacccttac cgctggcgga gcaacgtcag tggcaaagtc
     4321 agttgccggg tattgcagat aattgcccgg caatagttcg cttgctgggc gatatgtcgc
     4381 cttcctacga tgctgccgcc tggaaccgtc tggcaaagtg ttatcgggac acgctacccg
     4441 gtgtggcgct gtatgcatgg cttcaggccg aacaacgaca accgaacgcc tggcaacatc
     4501 gtgcggtagc ctatcaggcg tatcaggttg aggactacgc caccgcactg gcggcctggc
     4561 agaaaatcag tcttcacgac atgagcaatg aggatctgct tgctgctgcc aataccgccc
     4621 aggcggcagg aaatggtgca gctcgcgatc gctggctaca acaggcagaa caacgtggac
     4681 tgggaaacaa tgccctctac tggtggctgc atgcgcaacg ttacattcct ggtcagccgg
     4741 aactcgcact gagcgatctc acgcgctcaa tcaatattgc gccttctgcc aacgcttacg
     4801 ttgcgcgggc gacaatttat cgccaacgtc ataatgtccc ggcggcggtg agtgatttgc
     4861 gcgccgcgct ggaactggaa ccgaataata gcaacatcca ggcagcgctt ggttacgcct
     4921 tgtgggatag cggtgatatc gcacagtcgc gggaaatgct cgaacaggcg cataaagggc
     4981 ttccggacga tccggcactg atccgacaac tggcctatgt gaaccagcgt ctggatgaca
     5041 tgcctgcgac gcagcattac gcccggctgg tgattgatga cattgataat caggcgctga
     5101 taaccccact gaccccagag caaaatcagc aacgcttcaa tttccgccgt ttgcatgagg
     5161 aggtcggtcg ccgctggacg ttcagtttcg attcttctat cggcttgcgt tccggggcaa
     5221 tgagcaccgc taacaataat gtcggcggcg cagcgccagg gaaaagctat cgtagctacg
     5281 ggcaactgga agccgagtac cgcatcggac gcaatatgct gctggaaggc gacctgctct
     5341 cagtttatag ccgcgtcttt gccgataccg gagaaaacgg ggtgatgatg ccggtgaaaa
     5401 atccgatgtc cggcaccggt ctgcgctgga agccgctgcg cgatcagatc tttttcctcg
     5461 ccgtcgaaca gcagttgccg ctgaacgggc aaaatggcgc atccgatacc atgctgcgcg
     5521 ccagcgcctc attctttaat ggcggcaaat acagcgacga atggcacccg aacggttcag
     5581 gctggtttgc ccaaaacctg tacctcgatg cggcgcaata tatccgccag gatattcagg
     5641 cgtggacggc agattatcgc gtcagctggc atcagaaggt agctaacgga cagactattg
     5701 agccttacgc tcacgttcag gacaacggct atcgtgataa aggcactcag ggcgcgcagc
     5761 ttggcggagt cggggtccgc tggaatatct ggaccggcga gacgcactac gacgcctggc
     5821 cgcacaaagt cagtctcggc gtcgagtatc aacatacctt taaggcgatt aatcaacgta
     5881 acggagagcg caacaacgcg tttctcacca ttggagtgca ctggtaaatg cgtaagttca
     5941 ttttcgtatt gctgacactg cttttggtca gccctttttc ctttgcgatg aaaggtatta
     6001 tctggcaacc acaaaaccga gatagtcagg ttaccgatac ccagtggcag gggctgatga
     6061 gtcagttacg tttgcaaggc ttcgataccc tggttttgca atggacccgt tacggcgatg
     6121 cattcaccca gccagaacag cgcgcgttat tgtttaagcg tgccgcagct gcgcaacagg
     6181 ctggcctgaa gctgattgtc gggctgaacg ccgatccgga attttttatg caccagaaac
     6241 agtcgtccgc agcgctggaa agctatctta atcgcctgct ggctgccgat ctccagcaag
     6301 ccagattatg gagcgccgcg cctggcgtaa cgccggatgg ctggtacatc agcgcggaaa
     6361 ttgacgacct gaactggcgc agcgaagctg cccgtcagcc tttgctaaca tggttaaaca
     6421 acgcgcagcg gctgattagc gatgtttcag caaaaccggt ttatatcagt agttttttcg
     6481 ccggaaacat gtcgcccgat ggctatcgcc aactgctgga acaagttaaa gcaaccggcg
     6541 ttaatgtctg ggtacaggat ggcagcggcg tggataaact taccgctgaa cagcgtgaac
     6601 gttatctaca ggccagcgcc gattgccaaa gtcccgcccc tgccagcggc attgtttatg
     6661 aactgtttgt cgccggcaaa ggcaaaacct ttacagcgaa accgaaaccg gatgcagaga
     6721 tcgcctcgct gttagcgaaa cgttcctctt gcggtaaaga cactctctat ttctctctgc
     6781 gctatttgcc cgtcgcgcac ggcattctcg agtattaaat ctcctccagg taagtcgggt
     6841 acgacctggc ttacctcttt cgctctcgtc agaattccat caagattaat tgctaaaaag
     6901 cgtgctaaag aaagaagatc gttatcggcg aacgggccga tcactcatgc aggaagcata
     6961 gatcctttga gcggagtttc tgatatctga ggagtgcgaa atgcaattga gcagcagtga
     7021 accttgcgtg gtgatcctga ccgaaaaaga ggtagaggta agcgtcaata accatgctac
     7081 gtttaccctt ccgaaaaact acctggccgc cttcgcgtgc aacaataacg tcattgaact
     7141 ctcaacgtta aatcacgtat taatccccca catcaaccgt aacatcatca acgattatct
     7201 gttgttttta aataagaact taacctgtgt aaagccctgg tcgcggctgg caaccccggt
     7261 tatcgcttgt catagccgta caccggaagt gttccggcta gccgccaacc acagcaagca
     7321 gcaacccagc aaaccctgcg aagcggagtt gacgcgcgca ttacttttta ccgtattgtc
     7381 taactttctt gagcaatcgc ggtttatagc cctactgatg tatatcttac gcagcagcgt
     7441 ccgcgacagc gtctgccgca ttattcaaag cgatattcag cattactgga atctgcgaat
     7501 tgtcgccagt tcgctatgtt taagccccag cctactcaaa aagaaattaa aaaacgaaaa
     7561 taccagctat agccagattg tcacagagtg tcgtatgcgt tacgccgtac agatgttatt
     7621 gatggataac aaaaatatca ctcaggtggc gcaattatgt ggctatagca gcacgtcgta
     7681 ctttatctct gtttttaagg cgttttacgg cctgacaccg ttgaattatc tcgccaaaca
     7741 gcgacaaaaa gtgatgtggt gaagggcaaa gcggaaacgg ataagacggg cataaatgaa
     7801 gaagaaatgg cgcgccctgc aggattcgaa cctgcggccc acgacttaga agttcctaga
     7861 acgacatttt aagtcaacaa attaccgcgc catctctgcg ctcacacgtc ccacgacctc
     7921 aaaacatgta aagccttgca agccattgtg aggccttatg tgtctcagtt ttgtccctct
     7981 tttttgtact aaaaaacata gcaattgagg ataaacctca tgctattttc gcttatatgc
     8041 ctttaaaggc atggcgctta aatagataaa aacaccacaa aagcataaaa aaccacacag
     8101 taaaaccaca acataaaaca ataacagata attaaaccaa aaacagatag cgcattgtga
     8161 taatcattca atactaaaca aaatataaac agtggagcaa tatgtgattg actcattaag
     8221 ttagatataa aaaatacata ttcaatcatt aaaacgattg aatggagaac ttttatgcgg
     8281 gcgaaacttc tgggaatagt tctgacaacc cctattgcga tcagctcttt tgcttctacc
     8341 gagactatat cgtttactcc tgacaacata aatgcggaca ttagtcttgg aactctgagc
     8401 ggaaaaacaa aagagcgcgt ttatctagct gaagaaggag gccgaaaagt cagtcaactc
     8461 gactggaaat tcaataacgc tgcaattatt aaaggtgcaa ttaattggga tttgatgccc
     8521 cagatatcta ttggggctgc gggctggact actctcggca gccgaggtgg caatatggtc
     8581 gatcaggact ggatggattc cagtaacccc ggaacctgga cagatgaaag taggcaccct
     8641 gatacacaac tcaattatgc caacgaattt gatctgaata tcaaaggctg gctcctcaac
     8701 gaacccaatt accgcctggg actcatggcc ggatatcagg aaagccgtta tagctttaca
     8761 gccagaggtg gttcctatat ctacagttct gaggagggat tcagagatga tatcggctcc
     8821 ttcccgaatg gagaaagagc aatcggctac aaacaacgtt ttaaaatgcc ctacattggc
     8881 ttgactggaa gttatcgtta tgaagatttt gagctaggtg gcacatttaa atacagcggc
     8941 tgggtggaag catctgataa cgatgagcac tatgacccag gaaaaagaat cacttatcgc
     9001 agtaaagtca aagaccaaaa ttactattct gttgcagtca atgcaggtta ttacgtaaca
     9061 cctaacgcaa aggtttatgt tgaaggcgca tggaatcggg ttacgaataa aaaaggtaat
     9121 acttcacttt atgatcacaa tgataacact tcagactaca gcaaaaatgg tgcaggcata
     9181 gaaaactata acttcatcac tactgctggt cttaagtaca ccttttaacg aagttaacca
     9241 gattttctcc ccggtggtat tgtataaccc cggggaattt gtttacagtt acaagtaggc
     9301 tatatccact aattttgtaa gcatttaacc tatgtcataa cgtgtaatct cttaccgcaa
     9361 cacaagacct caatatattc aaatcttatc tttattccga tattcagaga cacaatgcga
     9421 aaaataatta ctcatttcaa agttgtttta acgttacttc taccagtaac cgtatctgcc
     9481 cagcagatac agtggcaatc ctgtatggcc agtcaattca accactggtt tggtgaggaa
     9541 aaaccgtctc ctgacttact atgtggttat ttgtctgttc cattaaaata tacagacaca
     9601 ggcggagatg cttcttatga aaaaaaatca caagtcaaac tagcgttgac aaaattgccg
     9661 gcaaaaagca agcataaagg aagtatcctg ataataagtg gtggtcccgg gttaccaggc
     9721 ataaatcctt atattaactt tgactggcca gtcacaaatc ttcgtgagtc atgggatatt
     9781 attggatttg atcctcgagg cgtcggacag tccactccga caataaactg ccggcaatca
     9841 gatacagaga ctcaggaaaa cataaccgaa aagcaacaag tattaaataa aattaatgcc
     9901 tgtatccata ataccggagc cgaagtcatt cgccatatcg gctctaacga ggctgtatac
     9961 gatattgatc gtattaggca agccttgggg gataaacaac tgacagccgt ggcgtattcg
    10021 tatggaactc aaattgcagc cttatatgca gaacgttttc cctacaacgt aagatctatc
    10081 gttcttgatg gagtcgtcga tatcgatgac ctggaggaca acttcacatg gcaactcaaa
    10141 caggcacaga gttatcagga aacgtttgat cgctttgcat cctggtgtgc gcgtacaaaa
    10201 agttgcccgc tttcttcaga cagagataag gcaataactc agttccatga gctattatca
    10261 aaattacatc acaaaccttt attagacagt aagggagaaa atatatcttc agatgaactc
    10321 atatcattaa caacagacct tctgctatgg cgttcatcat ggccaaccct tgcaactgcc
    10381 atacgccagt tctctcaggg gattgtcagt aatgaaattg aaactgcgct cagtgctccg
    10441 atagcctcag aagagtcaag cgatgcttcg ggggtaatcc tctgtgtaga tcagggggat
    10501 gagcaattaa caccagaaga gcgaaaatcc cgaaaagacg ctcttgcgaa tgccttcccg
    10561 gctattaact ttgacaatgg acgttccgat tcacctgatt tttgtgaatt atggccaata
    10621 catagcgacc tgaacaaaac tcgcctgaaa aatactgttc tgccctctgg tttactgttt
    10681 gtagcacaca aatacgaccc aacaacgccc tggattaatg cccgtaagat ggcagagaaa
    10741 ttttccagcc cgttactaac aataaatggt gatgggcata cattagctct caccggagtt
    10801 aatttatgtg tagataaagc agttgtacat cacctgatca ctccacaaaa aatagaaaat
    10861 atatactgcc caggaaattc tgaagcagaa atacaataat ttcaaaatat tccgctattt
    10921 gctcccaatg caaaacatat attgcattgg gagcatattc atatttttg
//
LOCUS       DAJOWB010000139        10871 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJOWB010000139 DAJOWB010000000
VERSION     DAJOWB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.139"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..449
                     /locus_tag="OHO85_004038"
                     /pseudo
     CDS             1..449
                     /locus_tag="OHO85_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
     gene            773..2161
                     /gene="narU"
                     /locus_tag="OHO85_004039"
     CDS             773..2161
                     /gene="narU"
                     /locus_tag="OHO85_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCQ2673340.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2243..5983
                     /gene="narZ"
                     /locus_tag="OHO85_004040"
     CDS             2243..5983
                     /gene="narZ"
                     /locus_tag="OHO85_004040"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HCQ2673341.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSSSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5980..7524
                     /gene="narH"
                     /locus_tag="OHO85_004041"
     CDS             5980..7524
                     /gene="narH"
                     /locus_tag="OHO85_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCQ2673342.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7524..8219
                     /gene="narW"
                     /locus_tag="OHO85_004042"
     CDS             7524..8219
                     /gene="narW"
                     /locus_tag="OHO85_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCQ2673343.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            8216..8896
                     /gene="narI"
                     /locus_tag="OHO85_004043"
     CDS             8216..8896
                     /gene="narI"
                     /locus_tag="OHO85_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCQ2673344.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8975..9868
                     /locus_tag="OHO85_004044"
     CDS             8975..9868
                     /locus_tag="OHO85_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HCQ2673345.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGAAVILFHAEWAIKL"
     gene            complement(9964..10809)
                     /gene="nhoA"
                     /locus_tag="OHO85_004045"
     CDS             complement(9964..10809)
                     /gene="nhoA"
                     /locus_tag="OHO85_004045"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HCQ2673346.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIAHRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
BASE COUNT         2597 a         2840 c         2822 g         2612 t
ORIGIN      
        1 ataacccaca ttgatactaa taatacatcc gatattggaa ctttattccc cataataaaa
       61 cagagcaaaa cttaaatttt ttaaatgttt ctgggaagaa caattgccct actatgcagc
      121 tcatgttatt taatttgttt tccccagcac ttaagcttaa tactggcctg gcaattcttt
      181 cgcctggtgc atttgaagtt cactctgacg gaatagatgt ggataacgaa ttgtttcact
      241 atcctattaa taaagcatat accccatata atatacacac ttacaagaca gaggaagttg
      301 taaaccagag gaatataaaa gttaaaaaca tgaccttaga tgaaataaac aatacttact
      361 gtaataacga ttattacaat caggcaataa gagaggaacc gatagacctt ctggacagat
      421 cgttttcctc cagttcatgg cctttttagt gaataaatca tcactaattc tataagtgtc
      481 tggtcttgtt gataatatag ttaatatgta accctttaga ttcacgatct gaagggttga
      541 aacatgacat acgttcaaag attaaccccc gtttgaaggt tagcttgtta gttacattta
      601 gtaacacata tctggctact taatccccat tctgcccccg cggttacgct ttcttatcta
      661 ttctttcaat cagcacttca cccaattcat ccttaattct aataactggc tattgcgcct
      721 tacctcctgt tttgtctttt ttcattacca atgtgtgcat gtgaggaaca atatggcact
      781 gcaaaatgag aaaaatagtc gttatctttt acgcgactgg aaaccagaaa atcctgcctt
      841 ctgggaaaat aaaggaaaac atattgctcg aagaaacctc tggatatcag tcagttgtct
      901 acttcttgcc ttctgtgtct ggatgctatt tagcgcagtt accgttaatc tcaataaaat
      961 cggttttaat tttactaccg atcaactctt tttattaacc gcattaccct ccgtttctgg
     1021 cgcattattg cgtgttccct actcctttat ggtgcctata ttcggtggac gccgatggac
     1081 ggtttttagt actgcaatcc tgattattcc ttgcgtctgg ctcggaattg ccgtgcaaaa
     1141 tccgaatact ccttttggga tatttatcgt tatcgctttg ctatgcggtt ttgcaggtgc
     1201 aaactttgct tcgagcatgg gcaatatcag tttcttcttt ccaaaagcca aacaagggag
     1261 cgctcttggg attaatggcg gattaggaaa cttaggtgta agtgtaatgc agctggttgc
     1321 accgctggtc atttttgtac ctgtatttgc ctttctcggc gtcaatggcg taccgcaggc
     1381 cgacggttcg gtgatgtcgc tggcgaatgc cgcatggatt tgggtaccgc tactggcgat
     1441 tgccacgatc gctgcctggt cagggatgaa tgatatcgcc agttcacgcg cctcaattgc
     1501 cgaccagctc cctgtcttac aacgcctgca tctctggctg ctgagcctgc tttaccttgc
     1561 caccttcggt tcgtttatcg gtttttctgc gggttttgcc atgctggcaa aaacccagtt
     1621 cccggatgtg aatattctgc gcctggcgtt ctttggccca tttatcggtg ccatcgcgcg
     1681 gtcggttggt ggtgctattt ccgataagtt cggcggcgtg cgggtgacgt tgatcaactt
     1741 tatttttatg gcgattttca gtgccctgct gttccttacc ttaccgggca caggctccgg
     1801 taatttcatc gccttttacg ccgtatttat ggggctgttt ctgactgcgg gtctgggaag
     1861 tggttctact ttccagatga tcgccgtcat ctttcgccag ataaccattt atcgggtaaa
     1921 gatgaaaggc ggtagtgatg agcaagctca gaaagaagcc gtcaccgaaa cggcggcggc
     1981 tctgggcttt atctcagcca ttggcgcagt gggcggcttt tttattccgc aggcgtttgg
     2041 catgtcgctc aatatgaccg gctctccggt cggcgcgatg aaagtgtttt taatcttcta
     2101 catcgtttgt gtgctgctga cctggctggt ttatggtcgg cggaagttca gccaaaaata
     2161 agagatcgat gcctgatgta caaccgtgtc gcatccgaca aggcatcaga tcccaattca
     2221 ttcctggagc aggagtcatg tcatgagtaa acttttggat cgctttcgct acttcaaaca
     2281 aaagggcgaa acctttgccg atggtcacgg acaggtgatg catagcaacc gcgactggga
     2341 ggacagctat cgccagcgtt ggcagttcga caaaatcgtg cgttccaccc acggtgttaa
     2401 ctgtacaggc tcctgtagct ggaaaatcta cgttaaaaat ggtctggtga cctgggaaat
     2461 ccaacagacc gactacccgc gcactcgccc tgacctgccc aatcatgaac ctcgcggctg
     2521 cccgcgtggc gcaagttact cctggtatct ttacagcgct aaccgcctga aatacccgct
     2581 cattcgtaaa cgactgattg aactgtggcg cgaagccctc aagcaacaca gcgatccggt
     2641 actggcgtgg gcatcgatta tgaacgatcc gcaaaagtgc ctgagctaca aacaagtgcg
     2701 tgggcgcggc gggtttatcc gctccaactg gcaggaacta aaccagctga ttgccgccgc
     2761 taacgtctgg accatcaaaa cctacggccc ggatcgcgtt gccggtttct cgccgatccc
     2821 ggcgatgtcg atggtttctt acgccgccgg aacgcgttat ctgtcgctgc ttggcggcac
     2881 ctgtttaagt ttctacgact ggtattgcga cctgccgccc gcctcgccga tgacctgggg
     2941 cgagcaaacc gacgtaccgg aatctgccga ctggtataac tccagctaca tcatcgcctg
     3001 ggggtctaac gtaccgcaga cacgtacgcc ggacgcccac ttctttaccg aagtacgcta
     3061 caaaggcact aaaaccatcg ccattacccc tgactactct gaagtggcca aattgtgcga
     3121 ccagtggctg gcaccgaaac aaggcactga tagcgccctg gcgatggcaa tgggccatgt
     3181 gattttaaaa gagtttcatc tcgataatcc cagcgactac tttatcaact actgccgccg
     3241 ctacagcgac atgccgatgc tggtaatgct ggagcctcgc gacgatggta gctacgttcc
     3301 cgggcgcatg atccgcgcat ctgacctggt ggatggactg ggcgaaagca acaatccgca
     3361 gtggaaaacc gtagcagtta ataccgcagg tgaattggta gtgccgaacg gttcgattgg
     3421 tttccgctgg ggagaaaaag gcaaatggaa tctggaatcc attgccgccg gtacggaaac
     3481 cgaattgtcg ttaaccctgc tcggtcaaca tgacgctgtt gcaggcgtgg ccttccccta
     3541 ctttggcggc attgaaaatc cgcattttcg cagcgtaaaa cacaatccgg tgctggtgcg
     3601 ccaattgccc gttaaaaacc tgacgttagt cgatggcaac acctgtccgg tggtcagcgt
     3661 ttatgatttg gtactggcga attacggcct cgatcgcggg ctggaagatg aaaacagtgc
     3721 gaaagattac gctgaaatca aaccgtacac cccagcctgg ggtgagcaaa ttaccggcgt
     3781 gccgcgccag tatattgaaa ccatcgcccg tgaatttgcc gataccgccc ataaaacgca
     3841 tgggcgctcg atgattatcc tcggcgcagg tgttaaccac tggtatcaca tggacatgaa
     3901 ctaccgcggg atgatcaata tgctgatctt ctgcggttgt gtcgggcaaa gcggtggcgg
     3961 ctgggcacac tatgtcggcc aggaaaaact gcgcccacaa accggctggt tgccactggc
     4021 ctttgcgctc gactggaacc gaccaccgcg ccaaatgaac agcacctcgt ttttctacaa
     4081 tcattccagc caatggcgct atgaaaaagt ctctgctcag gagttacttt caccgctcgc
     4141 cgatgccagt aagtacagcg gtcatctgat tgatttcaac gttcgcgccg aacgtatggg
     4201 ctggctacct tctgcgccgc agctggggcg taacccgctc gggattaaag ctgaagccga
     4261 caaggccgga ttatccccca cagaatttac cgcccaggcg ctgaaatcgg gcgatttacg
     4321 tatggcctgc gaacaaccag atagcagcag caatcatccg cgtaatttgt ttgtctggcg
     4381 ctctaacctg cttggctcct ccggcaaagg ccacgagtat atgcagaagt atctgctggg
     4441 gaccgaaagc gggattcagg gcgaggaact cggtgccagc gacgggatca aaccggaaga
     4501 agtcgagtgg caaactgcag cgattgaagg caagctcgac ctgctggtga cgctcgactt
     4561 ccgcatgtcc agtacctgcc tgttctccga tatcgttctg cccaccgcca cctggtacga
     4621 aaaagacgat atgaacacct cggatatgca tccgtttatt catccgcttt ctgcggcggt
     4681 cgatccggcg tgggaatcac gcagcgactg ggaaatctac aaaggtatcg ccaaagcatt
     4741 ttcgcaagtg tgcgtgggtc atcttggcaa agaaaccgac gtggtattac aacccctact
     4801 gcatgactct ccggcagagc tctcacagcc gtgtgaagtg ctcgactggc gcaaaggcga
     4861 atgcgatctg attccaggca aaaccgcgcc gaatattgtg gcggtggagc gcgactaccc
     4921 tgctacgtat gaacgcttta cctcgctcgg gccattgatg gacaaacttg gcaatggcgg
     4981 taaagggatt tcgtggaata cgcaggatga aatcgatttc ctcggcaaac tcaattacac
     5041 caagcgtgat ggcccggcac aggggcgtcc gctgattgac accgccattg acgcttcaga
     5101 agtgattctg gcactggcac cggaaaccaa cggtcatgtt gcagtcaaag cgtggcaggc
     5161 gctgggcgag atcaccgggc gcgaacatac ccatctggcg ctgcacaaag aggacgagaa
     5221 gattcgcttt cgcgatattc aggcgcagcc gcgtaaaatt atctccagcc ccacctggtc
     5281 cggtctggaa agcgatcacg tatcctataa cgcgggatac accaacgttc atgagttaat
     5341 tccgtggcgc acgctgtcgg gacgccagca gctctatcag gatcatccgt ggatgcgtgc
     5401 ttttggtgaa agcctggtgg cttatcgccc gcctatcgac acccgtagcg tcagtgagat
     5461 gcgccagata ccgccaaacg gcttcccgga aaaagcactt aacttcctga cgccgcacca
     5521 gaaatggggc attcactcaa cctacagtga aaacctgcta atgctgacgc tctctcgcgg
     5581 tggaccgatt gtctggatca gcgaaacaga tgcccgagaa ctgaccattg tcgataacga
     5641 ctgggtggaa gtattcaacg ccaacggcgc gctgactgcc cgcgcggtgg tcagccaacg
     5701 tgtaccgccg ggcatgacca tgatgtatca cgcccaggaa cgcattatga atattcctgg
     5761 ttcggaagta actggcatgc gcggcggcat tcataactcg gtcacccgcg tttgcccgaa
     5821 accgacgcat atgattggcg gttacgcgca gctggcctgg ggctttaact actacggcac
     5881 cgtcggctcg aaccgcgacg aattcatcat gatccgcaaa atgaaaaacg ttaactggct
     5941 ggatgatgaa ggtcgcgatc aggtacagga ggcgaaaaaa tgaaaatacg ttcgcaagtc
     6001 ggcatggtgc ttaacctcga taaatgtatc ggctgccata cctgttcggt gacctgtaaa
     6061 aacgtctgga ccggacgcga aggcatggag tacgcatggt ttaacaacgt cgaaaccaaa
     6121 ccgggcattg gttatcctaa aaactgggaa gatcaggaag agtggcaagg cggctgggtc
     6181 cgcgatgtga atggcaagat ccgtccgcgt ctgggcagca agatgggcgt aataaccaaa
     6241 atcttcgcca acccggtggt gccgcagatt gatgattact acgaaccttt caccttcgac
     6301 tacgaacatt tgcatagcgc accggaaggc aaacatatcc ctactgctcg cccgcgttca
     6361 ctgattgacg gcaaacggat ggacaaagtg atctggggcc caaactggga agaactgctg
     6421 ggcggcgagt ttgaaaaacg tgcccgcgac cgcaacttcg aggccatgca aaaggagatg
     6481 tacgggcagt ttgaaaacac cttcatgatg tacctgccgc gcctgtgcga acactgcctc
     6541 aatcccagtt gcgtggcgac ctgcccaagc ggcgctatct acaaacgcga agaagacggc
     6601 attgtgctga ttgatcagga taaatgccgt ggctggcgtt tgtgcattag cggttgtccg
     6661 tacaaaaaaa tctacttcaa ctggaaaagc ggcaagtcag aaaaatgtat cttctgttat
     6721 ccgcgaattg agtcaggcca acccaccgtg tgctcagaaa cctgcgtggg tcgcatccgg
     6781 tatctgggcg tgctgcttta cgacgccgac cgcattgagg aagcggcgag caccgagcgc
     6841 gaagttgacc tctatgaacg tcagtgcgaa gtgttcctcg atccacacga tccctcagtg
     6901 atcgaggaag ccctgaaaca aggtattcca caaaacgtga ttgaagctgc ccagcgttcg
     6961 cctgtctaca aaatggcgat ggactggaaa ctggcgctac cgctgcaccc tgaatatcgc
     7021 accctgccaa tggtctggta cgttcctccg ctgtcgccga ttcagtccta cgcagatgcg
     7081 ggcggtttgc cgaaaagcga aggcgtgctg cccgccatcg aaagcctgcg tattccggtg
     7141 caatatctcg ccaatatgtt gagtgccggc gataccggtc cggtactgcg ggcgctgaaa
     7201 cggatgatgg cgatgcgcca ctatatgcgt tcacaaaccg tggaaggcgt tactgatact
     7261 cgtgccatcg acgaagtagg cctgagcgtc gcccaggtcg aagaaatgta tcgctatctc
     7321 gccattgcca actatgaaga tcgttttgtt atcccgacca gccatcggga aatggcgggc
     7381 gatgccttcg cagaacgcaa cggctgcggt tttacctttg gcgacggttg tcacggctcg
     7441 gacagcaaat tcaacctgtt caacagtagc cgtatcgatg ccatcaacat caccgaagtg
     7501 cgcgacaaag cggagggcga ataatgcaga tcctcaaagt gatcggcctg ttgatggagt
     7561 atccggatga gctgttgtgg gaatgcaagg aggacgcgct ggcgttgatc cgccgcgacg
     7621 cgccgatgct tacggatttc acccacaacc tgcttaacgc gccgctgctg gataaacagg
     7681 ccgaatggtg cgaagtgttt gaccgcgggc gcaccacgtc gctgctgctg ttcgaacatg
     7741 ttcatgccga gtcccgcgat cgcggccagg caatggtgga cctgctggcg gagtatgaaa
     7801 aggtcggcct gcagctggat tgtcgggaac tgcccgatta tctaccgctg tatctggagt
     7861 atttaagcgt actgcctgac gatcaggcga aagaaggatt gctcaatgtt gcgccgatcc
     7921 tcgccctgct tggcggtcgc ttaaaacaac gcgaggcacc gtggtacgcg ttgtttgatg
     7981 ctctgctgca actggcggga agctcccttt caagtgacag tgtcacaaaa caagtaaaca
     8041 gcgaagagcg cgatgacacc cgccaggcgc ttgatgcagt gtgggaagag gaacaggtga
     8101 agtttattga agataacgcc acggcgtgtg acagctcgcc gcttaatcaa tatcagcgac
     8161 gctttagcca ggatgtcgcg ccgcaatatg tcgacatcag tgcgggaggt gggaaatgat
     8221 tcagtatctg aacgtctttt tttacgatat ctacccgtac atttgtgcga cggtgttttt
     8281 cctcggcagc tggctgcgct acgactacgg gcagtacacc tggcgcgcct cctcaagtca
     8341 aatgctcgat aaacgcggga tggtgatatg gtcgaatctg ttccatatcg gcattttggg
     8401 gattttcttc gggcacttgt tcggcatgtt aacgccgcac tggatgtacg cgtggttttt
     8461 accagtggca gcgaaacaac tcatggcgat ggtgctcggc ggtatttgcg gtgtgttgac
     8521 gttgattggc ggcgcaggac tgctgtggcg caggctgaca aatcagcgcg tgcgtgccac
     8581 ttccactacg ccggatatca tcatcatgag cattttgctg atccagtgtc tgttggggct
     8641 aagtaccata ccgttttcag cacagtatcc tgatggtagc gaaatgatga agctggtggg
     8701 ttgggcgcaa agcattgtca ctttccgtgg tggatcgtca gagatgctca acggcgtagc
     8761 gttcgtcttc cgcctgcatc tggtgttggg aatgaccatc ttcctgctct tcccgttcac
     8821 ccgactggtg catgtgtgga gcgcgccgtt tgagtacttt actcgtcgat atcaaattgt
     8881 gcgttcgcgg cgataatttc accctggccg gatagccctt cgctctccgg ccacttgcag
     8941 gctatgatag ttctccagtc acaggagaac gttaatgaaa ccgcaggtgt accacgtcga
     9001 tgcttttacc tcacaaccgt ttcgcggcaa ttctgccggt gttgttttcc ccgccgataa
     9061 tcttagcgaa gcgcaaatgc agcttatcgc ccgcgagtta ggccattcgg aaactgcttt
     9121 tctgctgcac agcgacgaca gcgatgtgcg catccgctac tttacgccaa cggttgaagt
     9181 gccgatttgc ggtcacgcga cagtagctgc gcactatgta cgtgcgaagg tgttaggttt
     9241 aggaaattgc acgatctggc aaacatcgct ggcaggaaaa catcgcgtga ctatcgaaaa
     9301 gcacaacgat gattatcgta tttcgctgga acaaggtacg ccgggctttg aactgccgct
     9361 ggagggtgaa gtacgcgcgg cgattatcaa cgcattgcat cttactgaag acgatattct
     9421 gccgggtttg ccgatccagg tcgcgacgac cgggcattct aaagtgatga tcccattgaa
     9481 gccggaagtg gatatcgacg ctctttcgcc tgaccttaat gcgctgaccg ctatcagtaa
     9541 acagattggc tgcaatggtt tcttcccgtt ccagatccgc ccaggcaaaa acgaaaccga
     9601 tggtcgcatg ttctcgcctg cgattggtat tgtggaagat ccggtgactg gcaatgccaa
     9661 tggcccgatg ggtgcatggt tggtacatca caacgtattg ccccacgatg gcaaggtgct
     9721 gcgtgttaaa ggccatcagg ggcgcgcatt ggggcgtgac ggcgtgattg aagtgacggt
     9781 gacaattcgt gataaccaac cggaaaaagt caccatttct ggcgcggcgg tgattttgtt
     9841 tcatgccgaa tgggcaatta aactctgaaa ccgttgcctg atgcgcttcg cttatcaggt
     9901 ctacaaccga gctaatgtat gccggataag gcgtttacgc cgcatccggc aacctgacat
     9961 aaattatttt cccgcctccg ggtgcgtatc aaaagccgcc atcaccagcg ctaactcatc
    10021 cacggtaaag ccatgtttcg catcatccac gcccagacca aactgttctt gcatcacagc
    10081 atataatgac gccacatccg gtagatttcg ctgctccacc gcgtgcccat tttcataatg
    10141 ggtaaaatga aaattggtca gtgtcagctt gccgccgtcc ggcaaatggc ggcacatcag
    10201 caaatgatgg cgaaaatgcg actgcggcca gtgcgccgac cagaaattgc ccatcacata
    10261 atcgctttgt tgctgctcgc agagatcaaa acggtacatc gactgccagt gctgatgatg
    10321 attaaactgc aacacccaat catcaccctc ctgcaacaac cgataatctc cgtgtggcgt
    10381 ggtctgcacg agatcggaaa ctaaacgaat cggcgcggtt agcgtctgcc caccgaaacc
    10441 gacatcagca atccattttt cctcttccag ttccaccaac agcaaacgat gggtgcgcgg
    10501 cggtaatgct ggcggatttg ataacactac gcgccctaac aagctgcgaa cgttaaactc
    10561 cagctcgcgt aacacccgct caaacacgcc attctgctca aaacagtaac cgccacgatg
    10621 ggctatcacc agtttctctt ccagcgattg atcatcaagc tgtatttccc tcggcagcaa
    10681 aacgtcgagg ttttcaaacg gaatagtgca attgtgtttc aggtgcaatg cacgaagcgt
    10741 atcaatattg accgcagcag ctcccgacca gttaatacgg gcaaaatagt gattcagaat
    10801 gggcgtcatc aggcgtttcc cttagtggtt ttctcaactt ataacctgga atagacgccg
    10861 caccggctat t
//
LOCUS       DAJOWB010000140        10830 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJOWB010000140 DAJOWB010000000
VERSION     DAJOWB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.140"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(114..1160)
                     /gene="aroH"
                     /locus_tag="OHO85_004046"
     CDS             complement(114..1160)
                     /gene="aroH"
                     /locus_tag="OHO85_004046"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCQ2673347.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYYEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(1317..2150)
                     /gene="ppsR"
                     /locus_tag="OHO85_004047"
     CDS             complement(1317..2150)
                     /gene="ppsR"
                     /locus_tag="OHO85_004047"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCQ2673348.1"
                     /translation="MENAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEVLYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            2483..4861
                     /gene="ppsA"
                     /locus_tag="OHO85_004048"
     CDS             2483..4861
                     /gene="ppsA"
                     /locus_tag="OHO85_004048"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCQ2673349.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(4918..6564)
                     /gene="fadK"
                     /locus_tag="OHO85_004049"
     CDS             complement(4918..6564)
                     /gene="fadK"
                     /locus_tag="OHO85_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCQ2673350.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(6621..6914)
                     /locus_tag="OHO85_004050"
     CDS             complement(6621..6914)
                     /locus_tag="OHO85_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCQ2673351.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(6911..8200)
                     /locus_tag="OHO85_004051"
     CDS             complement(6911..8200)
                     /locus_tag="OHO85_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCQ2673352.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNNDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMELAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(8256..9194)
                     /locus_tag="OHO85_004052"
     CDS             complement(8256..9194)
                     /locus_tag="OHO85_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCQ2673353.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(9214..9978)
                     /locus_tag="OHO85_004053"
     CDS             complement(9214..9978)
                     /locus_tag="OHO85_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCQ2673354.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLTTDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            10294..>10830
                     /locus_tag="OHO85_004054"
     CDS             10294..>10830
                     /locus_tag="OHO85_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCQ2673355.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKD"
BASE COUNT         2618 a         2751 c         2795 g         2666 t
ORIGIN      
        1 atataacaat gattcttatt atcaatatta tggctgacca gagcaagcag taatgtgctg
       61 agaagacaac aagttgcgca gaaatgccca tcccatcagg aatgggcacg cattcagaag
      121 cgggtatcta ccgcagaggc gagtttttcg accaggcgtt cggtatcttc ccagcccaga
      181 cacgggtcgg taatggattg accgtaagtg agcggctgac cgccgacgat tttttgcgtt
      241 ccttcgcgca ggaaactttc cgccataatt ccagcaatcg ccgtagagcc attgcggatt
      301 tgctgacaaa tatcctcaca aacttctaac tggcgacggt gctgcttctg gcagttaccg
      361 tggctgaaat ccaccaccag atgttcaggt aaatcaaact cgtgcagcgt atcgcaggct
      421 gcggcgatat catcggcatg ataattcggt tttttgccgc cacgcataat aatgtggcca
      481 tacgggttgc cgctggtctg atagatggtc atctgaccat ttttgtctgg cgagagaaac
      541 atatggctgg cgcgggctgc gcggatagca tccacggcaa tccgcgtgtt gccatcggta
      601 ccatttttaa aacctaccgg acaggagagt gccgaagcca tttcacggtg gatctgactt
      661 tcggtagtac gtgcgccaat cgctccccaa ctgattaaat cagcaataaa ctgaccggtc
      721 accatatcga ggaactcagt cgcggtaggg acacccagct catttacctg taaaagtaat
      781 ttgcgcgcca gctccagacc gtgatttacc cgatagctgc cgtttaaatc tggatcggag
      841 attagccctt tccagccgac aacagttcgg ggtttttcat aataggtgcg cattacgatt
      901 tcaagccgtg actggtactg gttgcgcagc gactgcagac gggtggcgta ttccattgca
      961 gcggtgagat cgtggatcga gcaggggcca ataatgacca acagtcgctt atcttcacca
     1021 ttcagtattt tttcaattct gcggcgggag tcggtgacat gggtggcgac gccaggcgtt
     1081 acgggatacc gtagcgcgag ttcggcgggc gttaccaggc tctcaatacg cgcagtacgg
     1141 agttcgtcag ttctgttcat tacaagtctc agaaatttgt tgcttctacg gcaggactac
     1201 cgggaagtga tgcgcatcac gataaaccaa tcgcagctga tttcaagtgc gaggtgtgtc
     1261 acacctcgcc gggtggttag catgataaca aaaaaacaag ttaatgcact agttctctag
     1321 tacattcgac ggctaaggcc catgatatcg aggatcttgg tggcaatctc ttctaccgaa
     1381 taattggtac tgttaatcca cgggatctga tttttacggt acaacacttc cacttccgcg
     1441 acttccatcc tgcactgacg aagcgaggca tagcgactgt tctcccgacg ttcctcgcga
     1501 atcgccgcca gtcgttccgg gtcgatagtc aggccgaaca atttatgctg aagcggtttg
     1561 agcgacgcgg gtagcaccag attatccata tcgtcggcaa taaaggggta gtttgccgcg
     1621 cggataccaa actgcattgc cagatacaga ctggtggggg ttttaccaca gcgagaaaca
     1681 ccgagcagga tcacctgagc ctggtccaga ttgcgcaacg aaatgccgtc atcgtgggcg
     1741 agggtgtaat caatcgccgc aatgcgcgca tcatatttat tgagattatt agggttaagg
     1801 ccatgggtac gatgagcaat cggcgttgga tccagtttca tctcttgttg tagcggggca
     1861 accagcgcct gaacgatatc ctggcaaaaa ccttcacttt gcaagatgat ggcgcgaatc
     1921 tccggcaaca cgatggagta gaagaccagc gggcgcacac ctgtctggtg ataaattgcg
     1981 tcaatctgat ccttcactgc ccgtgcacgg ctctcatttt cgacaaacgg cagcgtaatg
     2041 ctgctgatag tgacgggaaa ttgtgacatt actgcgtgtc ctaatacctc cgcagttatt
     2101 gccgtaccat cagaaatata aaaaacgtgg cgatcaacag cattttccat tttgttcttc
     2161 ccgtgatgca gacataattc tatgaaagca taaattaaaa acgcacagaa gcgtaggacg
     2221 taatgtctgg tttataaaat gaaccttcaa ttttattttt tatgaaaaca gcatttcatt
     2281 tttatggttt cgtttatacc gatggtttat gtggaaattg tcgaatagag caggtttgcg
     2341 caacgctggg atcagtctta aaaagtaaaa aaatatattt gcttgaacga ttcaccgttt
     2401 ttttcatccg gttaaatatg caaagataaa tgcgcagaaa tgtgtttctc aaaccgttca
     2461 tttatcacaa aaggattgtt cgatgtccaa caatggctcg tcaccgctgg tgctttggta
     2521 taaccaactc ggcatgaatg atgtagacag ggttgggggc aaaaatgcct ccctgggtga
     2581 aatgattact aatctttccg gaatgggcgt ttccgttccg aatggtttcg ccacaaccgc
     2641 cgacgcgttt aaccagtttc tggaccaaag cggcgtaaac cagcgcattt atgaactgct
     2701 ggataaaacg gatattgacg atgttaccca gcttgcgaaa gcgggcgcgc aaatccgcca
     2761 gtggattatc gacactccct tccagcctga gctggaaaat gccatccgcg aagcttatgc
     2821 acagctttcc gccgatgacg aaaacgcctc ttttgcggtg cgctcctccg ctactgcaga
     2881 agatatgccg gacgcttcct ttgccggtca gcaggaaacc ttcctcaacg ttcagggttt
     2941 tgacgccgtt ctcgtggcag tgaaacatgt atttgcttct ctgtttaacg atcgcgccat
     3001 ctcttatcgt gtgcaccagg gttacgatca ccgtggcgtg gcgctctccg ccggtgttca
     3061 acggatggtg cgctctgacc tcgcatcttc tggcgtgatg ttctccattg ataccgaatc
     3121 cggctttgac caggtggtgt ttatcacttc cgcatggggc cttggtgaaa tggtcgtgca
     3181 gggcgcggtt aacccggatg agttttacgt ccataaaccg acacttgcag cgaatcgccc
     3241 ggctatcgtg cgtcgcacca tggggtcgaa aaaaatccgc atggtttacg cgccgaccca
     3301 ggagcacggc aagcaggtta aaatcgaaga cgtaccgcag gaacagcgtg acatcttctc
     3361 gctgaccaac gaagaagtgc aggaactggc aaaacaggcc gtacaaattg agaaacacta
     3421 cggtcgcccg atggatattg agtgggcgaa agatggccac accggcaaac tgttcattgt
     3481 gcaggcgcgt ccggaaaccg tgcgctcacg cggtcaggtc atggagcgtt atacgctgca
     3541 ttcacagggt aagattatcg ccgaaggccg tgctatcggt catcgcatcg gcgcgggtcc
     3601 ggtgaaagtc atccatgaca tcagcgaaat gaaccgcatc gaacctggcg acgtgctggt
     3661 cactgacatg accgacccgg actgggaacc gatcatgaag aaagcatctg ccatcgtcac
     3721 caaccgtggc ggtcgtacct gtcacgcggc gatcatcgct cgtgaactgg gcattccggc
     3781 ggtagtgggc tgtggtgatg caacagaacg gatgaaagac ggtgagaacg tcactgtttc
     3841 ttgtgccgaa ggtgataccg gttacgtcta tgcggagttg ctggaattta gcgtgaaaag
     3901 ctccagcgta gaaacgatgc cggatctgcc gttgaaagtg atgatgaacg tcggtaaccc
     3961 ggaccgtgct ttcgacttcg cctgcctgcc gaacgaaggc gtgggccttg cgcgtctgga
     4021 atttatcatc aaccgtatga ttggcgtcca cccacgcgca ctgcttgagt ttgacgatca
     4081 ggaaccgcag ttgcaaaacg aaatccgcga gatgatgaaa ggttttgatt ctccgcgtga
     4141 attttacgtt ggtcgtctga ctgaagggat cgcgacgctg ggtgccgcgt tttatccgaa
     4201 gcgcgtcatt gtccgtctct ctgattttaa atcgaacgaa tatgccaacc tggtcggtgg
     4261 tgagcgttac gagccagatg aagagaaccc gatgctcggc ttccgtggcg cgggccgcta
     4321 tgtttccgac agcttccgcg actgcttcgc gctggagtgt gaagcagtga aacgagtgcg
     4381 caacgacatg gggctgacca acgttgagat catgatcccg ttcgtgcgta ccgtagatca
     4441 ggcgaaagcg gtggttgaag aactggcgcg tcaggggctg aaacgtggcg agaacgggct
     4501 gaaaatcatc atgatgtgtg aaattccgtc caacgccttg ctggcagagc agttcctcga
     4561 atatttcgac ggcttctcaa ttggctcaaa cgatatgaca cagctggcgc tcggtctgga
     4621 tcgtgactcc ggcgtggtgt ctgaattgtt cgatgagcgc aacgatgcgg tgaaagcatt
     4681 gctgtcgatg gctatccgtg ccgcgaagaa acagggcaaa tatgtcggga tttgcggtca
     4741 gggtccgtcc gaccatgaag actttgccgc atggttgatg gaagagggga tcgatagcct
     4801 gtctctgaac ccggacaccg tggtgcaaac ctggttaagc ctggctgaac tgaagaaata
     4861 aaataaatcc ccggcggcgt tcagtcgccg gggttatgtg atccccgaag atgatactta
     4921 ttcaatctct tcacagacat cctgcgttaa tcgccgcata atatcttttc ttaacaaaaa
     4981 cttttgtatt ttacctgagg cagtgcgcgg tagtttttcg attaccacga tatgttcagg
     5041 atatttatat tttgcgaccc gtttacggct aaaaaaagcc accacctctt ccagcgataa
     5101 tgaatgatgc ggcgctttca gcacgacata agcgcatgat cgttcaccta aacgttcatc
     5161 gggcattgca accacacagg catcgtgaat tttaggatgc tgcaataaaa tatcttccac
     5221 ttcacggctg ctaatgtttt cgccgccgcg gacaataata tcttttttgc gtccggtaat
     5281 ttttatatag ccagcctcat ccatgcggca gagatcgccg ctgtaatacc agccttcttc
     5341 gtccagcgca cgggcggtta attcaggttc atcaaaatac cccataaaca cattggggcc
     5401 acgcgaggct tcttcacctt cgcaacctgg cggtaaggtc ttgcgtgcgt catcgaccac
     5461 tttaatctct acacctgcgg cagcgtaacc atcggtgtgc ataaagcgcg acaaaggatc
     5521 atcgagattc accaccgcat gcggcgaact ttctgtggaa ccataaacac ttaataattt
     5581 aatgccgcgc tgctggcatt cacgtgccac ttttttgggg attgtggtac cgccgcaaag
     5641 aaagaaacgc agcgctgaaa ggtccgcggg ttgtttctct agtacattca aaagatcata
     5701 gacaaacggc gttgcgccta gcatacaggt gcaacgctgc tgctcaagca gcgcgagaca
     5761 cgcatcagga gtgaaaatat ctaacaacac gctgcgagcg ccaattaaga atggtgccgt
     5821 tacgccatgc agaaagcccg ttgcgtgacc aagtggcgca ggcatcataa agacatcctg
     5881 ccaggtcaga ttcagtcgcg cgcaataagc ccgctcactg gcgagaatat tgttatgcgt
     5941 tagcatcacg ccctttggca gaccctcggt tccggaggta aacagcaccg cagctaattc
     6001 atcgccgtgg gtcgttatcg ccgtggtcag tgaggtattg tcggcgataa tctgacttaa
     6061 tgagagggaa gaggtggcgg gagccagttt gtccacaccg acaatttgtt gtagttgtgg
     6121 aagctgattt tgcagcggca ggattaaatc taccggacgc gtttgtttaa acaacgtcgg
     6181 tgcaaagaac atttttgcct gacacttatt gagcacccac accagttctg cttcccgcca
     6241 ggaaggcaac agcggcacgg aaaccgcacc ggttttcagg caggcaagat agataacggt
     6301 aaattcacac cagcctggca gttgaaatgc gatgcgatcg cctgattcaa tacccttcgc
     6361 taacatccag tttgccagac agctcgcggc gtgatcgagc gcgctatagg tgtacgatgc
     6421 tccatgatta tcgaccacgg caattttgtc tggcatcgca cgggcggtct gctgccagta
     6481 atcggccagc gaagcatcgc cccatagccc ttgctgacga tacgccgcac gacgttgttc
     6541 gttgaacgtt aatgtcactt tcatgttgga ctctcgaaac agaacatcag gcccgagatg
     6601 cgggcctgtg ggatgcatga ttagccgtag cgaaaatcaa taccgaaggt gcctgcggga
     6661 tattgccact gttcgagaat agtgttaccg cacagcaccc gacaggtgcc gcactccaga
     6721 caaccggcag aatcaaaatg aatgttgccg gcgtcatcct gcttataaag tccggcaggg
     6781 caggctttca ttaatttacg gaattcattg atatcgggat ttgccgccag aatgatatgc
     6841 gggtggccct catcaacatg gaatttattg acgcctaatt tgatgtcgac gttaaccgta
     6901 gcgttctggc tcatagcgcg gttgctccct taatgccatc tttcagcaag ttgatcagcc
     6961 caattttctt cgcatgtccc atgatcattt tgcgtaccgg ctggtttggt ttgccgtcaa
     7021 tggtgaacat ctcgttcatg atgtcggcga ccattcgtgg gtattggcta aacaggcgcg
     7081 ggttttccat cagcgccggg atcttgcgga aatgctgcat atcgcgcatg acgcagcttt
     7141 gttccagctc acgtttgtat tgcgccagac tgctggcgga gaaatcctcg cgttctttgg
     7201 cggcgatcac cgttgtggcg gcagcctgag ccgatgcaat ggctaactcc atgccacgaa
     7261 ctgtaaaacc caaattcagg cagaagcctg cggcgtcacc aacgatcatc acgccatcgt
     7321 taaccagctg cggcaccatt gccagaccgc cttctggcac catatgcgcg gaatattcaa
     7381 gcagtttgcc gccgctaatt agtgggcgaa tggcggggtg ttgtttaaaa tcttccagca
     7441 tttgcggcac gcttttttgc gcatgggcga tatcacccaa tccacaaacc agccccaacg
     7501 atacggaatc gttgttggta tagaggaatc cgccacccat caggccgtca gaaggggaac
     7561 cggcaaacag ccaggcggca ccttcctcgc ccgtaatatt aaagcgatcg ttgatctgtt
     7621 ctggcgtgag gccaataacc tctttaacac caacagcgta atgatgcgga tcggaagcgg
     7681 gaaccattcc cagcgagcgg ccaagcatcg agttaacgcc atcagccaga atcaccacat
     7741 tcgcttcgag aatatcatcc ccagcctgca cgccagttac cttgtttcct tcacgaacca
     7801 gcgcatcgac gcgaacgcct gggataaact gcgcgcccgc ctgctcggct tgttccatca
     7861 accacgggtc cagacgatta cgcaatacgg tataagatgc gtgttgcgga acatctggct
     7921 gctcgcggtg aaaatcgagg gtaacggcgc tttcttcggt taagaaggag attttctcgc
     7981 gtgtgacctt gcgttctacc ggcgctgatg ctgcaaagcc tggaatgatt gcttcaagtg
     8041 tgtgggcata aagacgcccg ccggtcatgt ttttacatcc ggcactgtcg ccgcgttcta
     8101 tcaccaggac atccagcccg gctcgtgcca tgaccagtgc ggcaacgcta ccagcaacgc
     8161 ccgcaccgac cacaatggca tcaaatttgt catccgacat gcgttatgct cccttcccgc
     8221 tgcgtttaca gcgggagatg tggttgaaag gggaattagc ggctcaactg gctaatcagg
     8281 gcagggacga ctttgtagat atcgcccacc agaccgtagt cggcatagtt gaagattggc
     8341 gcatttttat ctttattgat ggcgacaatc acttttgcgc cgttgccgcc aaccatatgt
     8401 tggatctgcc cggagatccc cagcgtcagg tagagatcag atttcagcaa cacgccggag
     8461 acaccgatat aacgttcacg ctccatccag ttctcgcctt cggcaattgg acgtgaacag
     8521 ccgacttcag cattcagcac cgccgccagt tcgtggacca ttttcaggtc atcctgcgcc
     8581 gcaagaccac gaccgacgcc aaccacacgt tttgctttgc tcaggtccac gctgcttgcg
     8641 gctttcgcac ggcgttcgcg acagagaatt tcatgacgcg gagcaacata aggtaccgtt
     8701 tctgtcgggc actgatgaga ggtatcactg gtgcacggtt cctgaacacc gggtgcaagg
     8761 gtaatgatcg ccagcgggct gttgatcttt tcctgagcga acgctaaccc gccatacatc
     8821 cggtgttcgg cgcaaatgtg accatcgaca atatccaccg ccgtggcatc gttcaccagc
     8881 gccgcattca gttgcacact taaccgtgct gccagagctt taccacgttt cgtcgcggcc
     8941 aacagcaaca tagcggggtg tttatctttc agcagggcag caatgctttc ggcgtaattt
     9001 tcagtgcgtt gcagcgcgtc gttttgctca agaacataaa tacattttgg accataaggc
     9061 ataactgcct gcgcctggtc ggtattttgt acaatggcat acacctgttg gccccattgc
     9121 tgagcgccgc caaacagttc agcataacgt tcaggattat cgctaaagac ccagacgctg
     9181 tttaattgac tcatcgtttc ttctccgtag gcttcagttc agggctttct tcagatgttc
     9241 cgccagctcg gcaatggcct ccggcgaatc gttatcgata atgatgtgct tacgttctgt
     9301 ttgcggcggt acgcgaatgc cgaccagttc agcaagtggc gcgctctggc tccagtcaat
     9361 atcacttgcc tgccactgat ttaccggttt tttacccgcg ccgagaatgg ctttcatcga
     9421 aggaatgcgt ggcacgttaa tatcggaggt gacgcagagc acggctggaa cagagagttc
     9481 aataacttca acatcatctt caagcgtgcg ttcaatcacc agtgtattgc cctgacgctg
     9541 aatagcactc actgcattaa tcaccggaag ttgcagaatt tctccgacca gcaagccaac
     9601 ctgctgggca taaaggtcgc cggaaccttc accaaagatc agtaaatcga agccgatctt
     9661 ttcaattgct gccgccagcg cttttgcggt atcgagaggc agtgcatgtt caagttgcgc
     9721 atcctgcacc aaatacaggc tgtgcggccc gcgggatagc acgtctttgc gcactttcga
     9781 gttctgcaac aatgagccgc caacggtcag cgcggctatc tcatcgtcat ctgttgtgag
     9841 ctggcttgca gcttcaatgg cattgagatc gaactggctg attttggcgt cggcattgtc
     9901 gaaattcagg gtgtattctg gagtgacaac aatgtcctgt tcttcaggca ccagcttaaa
     9961 gcaggttatt attttcatga tttctcctgt gagtcgcata cggaaccatc tccgatggaa
    10021 ggtaaaaatg gcctgaaaat agtgtgcgat agcggtgcgg ggttttcgac ggagcaagct
    10081 gttttccttg ccaacaatct tgcaaaaaaa atgcgatcgc taaaacaaaa gtgcaatttg
    10141 cggcaatata gttattccat gaccgtacaa tcttgcattc agtgtaaata atcttgttta
    10201 gctctcgcta aacgcggttg tataaccgca atacacgccg tattttatcc ggcatatatt
    10261 gtttttgctt gctcatggaa tagggaggtt gtcatgtatc aacgctgttt tgataacgcc
    10321 agtgaaacgc tgtttgtcgc cggtaaaacg ccacggcttt cacgttttgc atttagcgat
    10381 gatccaaaat gggagtccgg acatcacgtt catgacaatg aaaccgagtt gatttacgtc
    10441 aagaaagggg ttgcaaggtt taccatcgat tcttcgttat atgtcgcaca tgcggatgac
    10501 attgtggtga tagaacgcgg caggctgcat gcggtggcct ctgacgttaa cgatccggca
    10561 acgacgtgta cctgtgcgct gtacggcttt cagtttcagg gggctgagga aaatcagcta
    10621 ctgcaaccgc attcttgtcc ggtaattgcc gcggggcagg gaaaagaagt cataaaaacc
    10681 ttatttaatg agctaagtgt gattttgccg caaagtaaaa atagccaaac atcttcgtta
    10741 tgggatgcat ttgcctatac gttagccatt ctttactacg aaaattttaa aaatgcttat
    10801 cgttcggagc aggggtatat taaaaaagat
//
LOCUS       DAJOWB010000141        10830 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJOWB010000141 DAJOWB010000000
VERSION     DAJOWB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.141"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            207..1421
                     /locus_tag="OHO85_004055"
     CDS             207..1421
                     /locus_tag="OHO85_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCQ2673356.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1488..2771
                     /locus_tag="OHO85_004056"
     CDS             1488..2771
                     /locus_tag="OHO85_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2673357.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            3023..3940
                     /gene="oxyR"
                     /locus_tag="OHO85_004057"
     CDS             3023..3940
                     /gene="oxyR"
                     /locus_tag="OHO85_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCQ2673358.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3923..5323)
                     /gene="sthA"
                     /locus_tag="OHO85_004058"
     CDS             complement(3923..5323)
                     /gene="sthA"
                     /locus_tag="OHO85_004058"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCQ2673359.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5654..6304
                     /gene="fabR"
                     /locus_tag="OHO85_004059"
     CDS             5654..6304
                     /gene="fabR"
                     /locus_tag="OHO85_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCQ2673360.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            6304..6663
                     /locus_tag="OHO85_004060"
     CDS             6304..6663
                     /locus_tag="OHO85_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCQ2673361.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(6703..7803)
                     /gene="trmA"
                     /locus_tag="OHO85_004061"
     CDS             complement(6703..7803)
                     /gene="trmA"
                     /locus_tag="OHO85_004061"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCQ2673362.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      7917..8107
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8172..10016
                     /gene="btuB"
                     /locus_tag="OHO85_004062"
     CDS             8172..10016
                     /gene="btuB"
                     /locus_tag="OHO85_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCQ2673363.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHFGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            9961..10818
                     /gene="murI"
                     /locus_tag="OHO85_004063"
     CDS             9961..10818
                     /gene="murI"
                     /locus_tag="OHO85_004063"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCQ2673364.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2483 a         2555 c         2902 g         2890 t
ORIGIN      
        1 atggagtttg ttatgtgatt atttaacgtg ttgttttaac gatggtggtt aattattcag
       61 gtggtttttg agggtatttt ttctcctcgg cggaaatttt tatttttaat gtgatcacgg
      121 ttgaataatc gctctttctc tcttgtatgg tagtaggcgt aaaattaagt ttgaagcgtc
      181 tatttacaac acaggagatt gcaataatga aaattattgc tgcagatgtt tttgttactt
      241 gcccagggcg taatttcgtt acgcttaaaa taaccactga aagcggactt tgtggattag
      301 gagatgccac gttaaatggt cgcgaattgt cggtcgcttc ttatctgaaa gatcatcttt
      361 gtccacaact aattggccgc gatgccagcc gtatcgaaga tatctggcaa ttcttttata
      421 aaggtgctta ctggcgacgt ggccccgtca cgatgtcagc catttctgcc attgatatgg
      481 cgttgtggga tatcaaagct aaagcggcaa atatgccatt gtatcaactg cttggtggtg
      541 cttctcgtga aggcgtgatg gtttattgcc acaccaccgg gcggacgatt gatgaagtcc
      601 ttgaagacta tgcaaagcac cagcagatgg gctttaaggc gattcgtgtg cagtgcggtg
      661 taccgggtat gcaaactact tatggcctgg cgaagggcaa aggcctggcg tatgaaccgg
      721 caaccaaagg cctgtggcca gaagagcagt tgtggtcttc agaaaagtat ctcgatttta
      781 cgccgaagtt atttgaagcg gtgcgtaata agttcggttt taacgaacat ctgttgcatg
      841 acatgcacca tcgcctgacc cccatcgaag cggcgcgctt tgggaagagc attgaggatt
      901 accgcctgtt ctggatggaa gatccgacgc ctgcagagaa ccaggaatgt tttcgtctga
      961 ttcgccagca taccgtgacg ccgattgcgg tcggtgaagt gtttaacagt atctgggact
     1021 gcaagcagtt aatcgaagag cagctgattg actatattcg cacgacgatc acccatgcag
     1081 gcggcattac cgggatgcgg cggattgccg attttgcatc gctttatcag gtacgtaccg
     1141 gttcgcatgg cccttcagat ttatcgcctg tttgtcatgc ggcggcgctg cattttgacc
     1201 tatgggtacc aaattttggc gttcaggagt acatgggcta ttccgaacaa atgcttgaag
     1261 ttttcccgca cagctggcgc ttcgaggaag gttatatgca cccaggcgat gaaccggggc
     1321 tgggtatttc tttcgatgag aaactggcgg cgaaatatcc gtatgacccg gcgtatctcc
     1381 ccgtcgcacg actggaagat ggcacattgt ggaactggta ataacggttc gcttaattat
     1441 tttctgaata gtcatcaaat cgattaattc cgctcaagga gctaaaaatg gacgttgatg
     1501 tgtcaaccag tgttgcgggt aataaaccgc aacggattcg tcgtattcag acagtgaccc
     1561 tggttttatt atttatggcg gggatcgtta attttctcga ccgttcgtca ttgagcgtgg
     1621 caggtgaagc gattcgtggc gagttaggat tatcggcgac agagtttggt gttttgcttt
     1681 ctgcattttc tctgtcttat ggtttttcac aactaccttc cggtattttg ctagaccgct
     1741 ttggcccacg aattgtttta ggcgcaggct taatattctg gtcattaatg caggcattaa
     1801 caggaatggt taattctttt agccacttta ttttaatgcg tatcggtctg ggtattggcg
     1861 aagcgccatt tatgcctgcg ggagtaaagt cgatcaccga ctggtatgca caaaaagaac
     1921 gcggcacggc gctggggatt tttaactcat ctaccgttat cggtcaggct atcgcgcctc
     1981 ctgctcttgt attgatgcag ctggcatggg gctggcggac gatgttcgtt atcatcggcg
     2041 tggcagggat tctggttggg atctgttggt acgcgtggta tcgcaaccgg gcgcagtttg
     2101 tcctgactga cgaagaacgg acgtatctct ccgcgccggt taaaccgcgt ccacagctgc
     2161 aatttagcga gtggctggcg ctgtttaagc atcggacaac ctgggggatg attttaggtt
     2221 tctctggcgt caattatacc ggttggctct acatcgcgtg gttacccggt tatttgcaag
     2281 ccgagcaagg tttcagtctg gcgaaaaccg gttgggtggc ggcgattcct ttcctcgcgg
     2341 cggcagtcgg gatgtgggtt aacggtattg ttgtcgatcg actggcgaaa aaaggctacg
     2401 acctggcgaa gacgcgtaaa acggctattg tctgcggttt gatgatgtcg gcattaggca
     2461 cgttgctggt cgtgcaatct tcctcgccag cccaggcggt agcgtttatc tcaatggcgc
     2521 tattctgcgt gcatttcgct ggaacgtctg catgggggct ggtgcaggtg atggtgtcag
     2581 aaacaaaagt ggcttccatc gccgggattc aaaactttgg cagttttgtt tttgcttcct
     2641 ttgctccgat cgtaaccggt tgggtagtgg ataccacaca ctcgtttaat ctggcgttgg
     2701 ttatcgcggc ctgtgtgacg ttcaccgggg cgctgtgtta cttctttatt gtcaaagatc
     2761 gcattgagta atcgttacta tcaggctctc ttgctgtggg cctgtagaat aaaaaaaagc
     2821 ggatcctgga gatccgcaaa agttcacgtt ggctttagtt attcgagttg agaaactctc
     2881 gaaacgggca gtgacttcaa gggttaaaag aggtgccgct ccgtttctgt gagcaattat
     2941 cagtcagaat gcttgatagg gataatcgtt cattgctatt ctacctatcg ccatgaacta
     3001 tcgtggcgat ggaggatgga taatgaatat tcgtgatctt gagtacctgg tggcattggc
     3061 cgagcaccgc cattttcggc gtgcggcaga ttcctgccac gttagccagc cgacgcttag
     3121 cgggcaaatt cgtaagctgg aagatgagtt gggcgtgatg ttgctggagc ggaccagccg
     3181 taaagtgttg ttcacccagg cgggaatgct gctggtagat caggcgcgta ccgtactgcg
     3241 tgaggtgaaa gtccttaaag agatggcaag ccagcagggc gagacgatgt ccgggccgct
     3301 gcacattggt ttgattccca cagttggacc gtacctgcta ccgcatatta tccctatgct
     3361 gcaccagacc tttccaaagc tggaaatgta tctgcatgaa gcacagaccc accagttact
     3421 ggcgcaactg gacagcggca aactcgattg cgtgatcctc gcgctggtga aagagagcga
     3481 agcattcatt gaagtgccgt tgtttgatga gccaatgttg ctggctatct atgaagatca
     3541 cccgtgggcg aaccgcgaat gcgtaccgat ggccgatctg gcaggggaaa aactgctgat
     3601 gctggaagat ggtcactgtt tgcgcgatca ggcaatgggt ttctgttttg aagccggggc
     3661 ggatgaagat acacacttcc gcgcgaccag cctggagacg ctgcgcaaca tggtggcggc
     3721 aggtagcggg atcactttac tgccagcgct ggctgtgccg ccggagcgca aacgcgatgg
     3781 ggttgtttat ctgccgtgca ttaagccgga accacgccgc actattggcc tggtttatcg
     3841 tcctggctca ccgctgcgca gccgctatga gcagctggca gaggccatcc gagcaagaat
     3901 ggatggccat ttcgataaag tgttaaaaca ggcggtttaa accgtttaac gcagctaccc
     3961 gataggcttc cgccatggtc gggtagttaa aggtggtgtt gacgaagtac tcaatagtgt
     4021 tgccgccacc tttctgttcc ataatcgcct gaccgatatg aataatttcg gcagcgcgct
     4081 cgccaaagca gtgaataccc agaatctctt ttgtttcccg atggaacaaa attttcagcg
     4141 tgcccacgtt catgccgacg atttgtgcgc gtgccagatg tttaaactgg gcgcggccca
     4201 cttcatatgg cactttcatc gcggtcagct gctgttcggt tttgcccaca gagctgattt
     4261 ccgggatggt gtaaatgccg gtagggatat cttcaatcag atgcgcggtg gcttcgcctt
     4321 ttaccagcgc ctgcgcggca atgcgcccct ggtcataggc cgctgatgcc aggctcggat
     4381 aaccaatcac gtcgcccacc gcgtaaacgt gcggctgtgc ggtctgatac atgctgttga
     4441 ccttcagctg tccgcgactg tcagtttcca gcccaatgtt ctgtaacgcc agcgaatcgg
     4501 tattaccggt acgaccgttg gcatagagca ggcagtcagc tttcagtttt ttacccgact
     4561 tcagatgcat gatcacaccg tcgtcacagc cttcgatctt ctcgtactct tcgttgtgac
     4621 gaatcactac gccactgttc cagaagtgat aggagagaga atctgacatc tcttgatcga
     4681 gaaatgccag caggcgatcg cgggtgttga tcagatccac ttttacatcc ataccgcgga
     4741 agatcgacgc atattcacag ccgatcactc cagcaccata gataagtaca tggcgcggtt
     4801 cgtggtgcat gctaagaatt gagtcgctgt cgtaaatgcg tggatgggtg aaatcaacat
     4861 ctgttggatg atatggacga gagccgcagg caataacaaa tttttcagcg gttagtgttt
     4921 caacgctgcc gtccgggcaa tccagcgcca acgtatgctc gtcaacaaag cgagcgtttc
     4981 cctgcaatat ttcacagtga ttacgttcgt aaaatccctg acgcatgcgc gtttgttgat
     5041 taatcacgtt atcggcatgg ttaaggatat cggcaaaaga agagcggagc agtcgggaat
     5101 ggtcgctgta aagtgggttt tgattgaatt ctataatgcg gctgacggcg tgacggagag
     5161 ctttcgacgg gatggtgccc cagtgggtgc aaccgccgcc aacattttga taacgctcga
     5221 taactgcgac gcgcgcacct tgcttaacca ggcccattgc agcgccttcg ccgccggggc
     5281 cggaacctat tactatggca tcgtaatcgt aggaatgtgg catggtaggg cttacctgtt
     5341 cttatacata aaagcaacag aatggtaaca ttttatcgcg ggtaagccaa ttgatccccg
     5401 tcatttatct ggctatatcc tgagcggcct ttgctttgtc tgtttcttac ttttgccctg
     5461 acgttttatt ggatttttat cgacgatact ctccgtttaa gcggcaggtt tccgctgtac
     5521 gtaaaagaac cggccaaaga attgcagtaa atatgtttta ttgcgttacc gttcattcac
     5581 aatactggag caatccagta tgttcattct ctggtatagt gccagcagta cttttggcaa
     5641 ggattcagac atcgtgatgg gcgtaagagc gcaacaaaaa gaaaaaaccc gccgttcgct
     5701 ggtggaagcc gcatttagcc aattaagtgc tgaacgcagc ttcgccagcc tgagtttgcg
     5761 tgaagtggcg cgtgaagcgg gcattgctcc cacctctttt tatcggcatt tccgcgacgt
     5821 agacgaactg ggtctgacca tggttgatga gagcggttta atgctacgcc aactcatgcg
     5881 ccaggcgcgt cagcgtatcg ccaaaggcgg gagtgtgatc cgcacctcgg tctccacatt
     5941 tatggagttc atcggtaata atcctaacgc cttccggtta ttattgcggg aacgctccgg
     6001 cacctccgct gcgtttcgtg ccgccgttgc gcgtgaaatt cagcacttca ttgcggaact
     6061 tgcggactat ctggaactcg aaaaccatat gccgcgtgcg tttactgaag cgcaagccga
     6121 agcaatggtg acaattgtct tcagtgcggg tgccgaggcg ttggacgtcg gcgtcgaaca
     6181 acgtcggcaa ttagaagagc gactggtact gcaactgcga atgatttcga aaggggctta
     6241 ttactggtat cgccgtgaac aagagaaaac cgcaattatt ccgggaaatg tgaaggacga
     6301 gtaatgaaac aagcaaatca agatagaggt acgctgctgc tggcgttggt tgctggctta
     6361 tcgattaatg gtactttcgc agcgctgttt agctccattg tgccattttc tgtattcccg
     6421 attatttccc tggtgctgac ggtttactgc ctgcatcaac gttatcttaa tcgcaccatg
     6481 ccggtaggct tgccgggtct ggcagctgcc tgttttattc tcggcgtact gctgtacagc
     6541 acggtagttc gtgcggaata tccggatatc ggctctaact tcttcccggc agtactctcc
     6601 gtcattatgg tgttctggat tggcgcgaag atgcgtaacc gtaagcagga agttgctgag
     6661 taatcggtat tatgccggat gcgccagcat ccggcatggg ttttacttcg cggtcagtaa
     6721 tacgccgcac tccatatggt gcgtgtaggg gaactgatca aacagagcca gacgttcgac
     6781 cttgtgcgtc tggcttaatg tttccagatt cttgcataac gtttccggat tgcaggagat
     6841 atacagaata cgtggatacg cctgcaccat tttctcggtt tcactgtcca gaccgctgcg
     6901 cggtgggtcg acaaaaatgg tttcgcactg ataactcttc aagtctatcc cttgcaggcg
     6961 attaaactcg cgtacaccat tcatcgcctg agtaaattct tctgccgcca tacgaataat
     7021 ttgtacgtta tcaatatggt tagctgcgat gttgtattgc gcagcagcaa ccgacggctt
     7081 agcgatttcg gtggctaata cccgatcaaa attgcgcgcc agtgctaatg aaaagttacc
     7141 gttgccgcag tacagctcca gtaaatcgcc ttttgagcct ttggttacgt ccagcgccca
     7201 ttccagcatc tgaatattca tcgccgcgtt cggctgggta aaactgtttt ctacctgacg
     7261 gtagatcatc tctttccctg cgaccggcag acgttcatcg atgtaatctt gatccagctc
     7321 gattttggtt ttcgttgccc gaccaatcag atgcacattc agattctgcg cgcgcagtgc
     7381 atcgcgcagg gcttccgctt cctgacgcca ctcatcatcc agctttttat ggtacaacag
     7441 ggaaaccacc gcctgattac tcagcgtggt gaggtaatca atctggaaca acttgtggcg
     7501 cagaacggga ttattacgca cacccgcaat catcgccgtc atcaactggt tgatgagttc
     7561 actggcagcg gggaagctat ccacgcggat gcggcttttg gtttgttgat cgaaaatgat
     7621 gtgatacagg tcatcgccat cgtgccagat gcggaactcc gcacgcatcc ggtaatgact
     7681 gaccggcgag cgaaacactt ccggaaccag gtcagaaaac ggtgccatca tactttgcaa
     7741 acgtaccact ttttcggcta actgcgcttc atactgttct gttggaaggt gttcgggggt
     7801 catgatgtat cctgaaaaat taaagtacgc ggcgattgta gggattgctc atcagatgtc
     7861 cagatcttga tgaattccta tttgtgagct acgtctggac agtaacttgt tacaacctgt
     7921 agcatccact tgccggtcct gtgagttaat agggaatcca gtgcgaatct ggagctgacg
     7981 cgcagcggta aggaaaggtg cgatgattgc gttatgcgga cactgccatc cggtgggaag
     8041 tcatcatctc ttagtatctt agatacccct ccaagcccga agacctgccg gccaacgtcg
     8101 catctggttc tcatcatcgc gtaatattga tgaaacctgc ggcatccttc ttctattgtg
     8161 gatgctttac aatgattaaa aaagcttcgc tgctgacggc gtgttctgtc acagcctttt
     8221 ccgcttgggc acaggatacc agcccggata ctctcgtcgt tactgctaac cgttttgaac
     8281 agccgcgcag cactgtgctt gcaccaacca ccgttgtgac ccgtcaggat atcgaccgct
     8341 ggcagtcgac ctcggtcaat gatgtgctgc gccgtcttcc gggcgtcgat atcacccaaa
     8401 acggcggttc aggtcagctc tcatctattt ttattcgcgg tacaaatgcc agtcatgtgt
     8461 tggtgttaat tgatggcgta cgcctgaatc tggcgggggt gagtggttct gccgacctta
     8521 gccagttccc tattgcgctt gtccagcgtg ttgaatatat ccgtgggccg cgctccgctg
     8581 tttatggttc cgatgcaata ggcggggtgg tgaatatcat cacgacgcgc gatgaacccg
     8641 gaacggaaat ttcagcaggg tggggaagca atagttatca gaactatgat gtctctacgc
     8701 agcaacaact gggggataag acacgggtaa cgctgttggg cgattatgcc catactcatg
     8761 gttatgatgt tgttgcctat ggtaataccg gaacgcaagc gcagacagat aacgatggtt
     8821 ttttaagtaa aacgctttat ggcgcgctgg agcataactt tactgatgcc tggagcggct
     8881 ttgtgcgcgg ctatggctat gataaccgta ccaattatga cgcgtattat tctcccggtt
     8941 caccgttgct cgatacccgt aaactctata gccaaagttg ggacgccggg ctgcgctata
     9001 acggcgaact gattaaatca caactcatta ccagctatag ccatagcaaa gattacaact
     9061 acgatcccca ttttggtcgt tatgattcgt cggcgacgct cgatgagatg aagcaataca
     9121 ccgtccagtg ggcaaacaat gtcatcgttg gtcacggtag tattggtgcg ggtgtcgact
     9181 ggcagaaaca gactacgacg ccgggtacag gttatgttga gaatggatat gatcaacgta
     9241 ataccggcat ctatctgacc gggctgcaac aagtcggcga ttttaccttt gaaggcgcag
     9301 cacgcagtga cgataactca cagtttggtc gtcatggaac ctggcaaacc agcgccggtt
     9361 gggaattcat cgaaggttat cgcttcattg cttcctacgg gacatcttat aaggcaccaa
     9421 atctggggca actgtatggc ttctacggaa atccgaatct ggacccggag aaaagcaaac
     9481 agtgggaagg cgcgtttgaa ggcttaaccg ctggggtgaa ctggcgtatt tccggatatc
     9541 gtaacgatgt cagtgacttg atcgattatg atgatcacac cctgaaatat tacaacgaag
     9601 ggaaagcgcg gattaagggc gtcgaggcga ccgccaattt tgataccgga ccactgacgc
     9661 atactgtgag ttatgattat gtcgatgcgc gcaatgcgat taccgacacg ccgttgttac
     9721 gccgtgctaa acagcaggtg aaataccagc tcgactggca gttgtatgac ttcgactggg
     9781 gtattactta tcagtattta ggcactcgct atgataagga ttactcatct tatccttatc
     9841 aaaccgttaa aatgggcggt gtgagcttgt gggatcttgc ggttgcgtat ccggtcacct
     9901 ctcacctgac agttcgtggt aaaatagcca acctgttcga caaagattat gagacagtct
     9961 atggctacca aactgcagga cgggaataca ccttgtctgg cagctacacc ttctgaacca
    10021 cgtcccaccg tgctggtgtt tgactccggc gtcggtgggt tgtcggtcta tgacgagatc
    10081 cggcatctct taccggatct ccattacatt tatgctttcg ataacgtcgc ttttccgtat
    10141 ggcgaaaaaa gcgaagagtt tattgttgag cgagtggtgg caattgtcac cgcagtgcaa
    10201 gaacgttatc cccttgcgct ggctgtggtc gcttgcaaca ccgccagtac cgtttcactt
    10261 cctgcattac gcgaaaagtt cgacttcccg gttgttggtg tcgtgccggc gattaaacct
    10321 gctgcacgtc tgacggcaaa tggcattgtc ggattactgg caacccgcgg aactgttaaa
    10381 cgttcttata ctcatgagct gatcgcgcgt ttcgctaatg aatgccagat agaaatgctg
    10441 ggctcggcag agatggttga gttggctgaa gcgaagctac atggcgaaga tgtttctctg
    10501 gatgcactaa aacgtatcct acgcccgtgg ttaagaatga aagagccgcc agataccgtt
    10561 gtattgggtt gcacccattt ccctctacta caagaagaac tcttacaggt gctgccagag
    10621 ggaacccggc tggtggattc tggcgcagcg attgctcgca gaacggcctg gctgttagaa
    10681 catgaagctc cggatgcaaa atctgccgat gcgaatattg ccttttgtat ggcaatgacg
    10741 ccagaagctg aacaattatt gcccgtttta cagcgttacg gcttcgaaac gctcgaaaaa
    10801 ctggcagttt taggctgatt tggttgaatg
//
LOCUS       DAJOWB010000142        10703 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJOWB010000142 DAJOWB010000000
VERSION     DAJOWB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.142"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(46..156)
                     /locus_tag="OHO85_004064"
     CDS             complement(46..156)
                     /locus_tag="OHO85_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673365.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
     gene            complement(209..613)
                     /locus_tag="OHO85_004065"
     CDS             complement(209..613)
                     /locus_tag="OHO85_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HCQ2673366.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(834..1565)
                     /gene="bluR"
                     /locus_tag="OHO85_004066"
     CDS             complement(834..1565)
                     /gene="bluR"
                     /locus_tag="OHO85_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HCQ2673367.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            complement(1770..2981)
                     /gene="bluF"
                     /locus_tag="OHO85_004067"
     CDS             complement(1770..2981)
                     /gene="bluF"
                     /locus_tag="OHO85_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HCQ2673368.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQCDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDFYTLSPNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYKAD
                     LKSKALAFAMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            3295..3531
                     /locus_tag="OHO85_004068"
     CDS             3295..3531
                     /locus_tag="OHO85_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HCQ2673369.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            3574..3846
                     /locus_tag="OHO85_004069"
     CDS             3574..3846
                     /locus_tag="OHO85_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HCQ2673370.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRIKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            3875..4141
                     /gene="ariR"
                     /locus_tag="OHO85_004070"
     CDS             3875..4141
                     /gene="ariR"
                     /locus_tag="OHO85_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HCQ2673371.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            4254..4502
                     /locus_tag="OHO85_004071"
     CDS             4254..4502
                     /locus_tag="OHO85_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673372.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            4834..6357
                     /locus_tag="OHO85_004072"
     CDS             4834..6357
                     /locus_tag="OHO85_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCQ2673373.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNSPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAIINGEIVPFYQPVVNGLEGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNYRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            6489..6707
                     /locus_tag="OHO85_004073"
     CDS             6489..6707
                     /locus_tag="OHO85_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673374.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
     gene            7464..9785
                     /locus_tag="OHO85_004074"
     CDS             7464..9785
                     /locus_tag="OHO85_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCQ2673375.1"
                     /translation="MRGNNTIVTTGDYSVGLLSQVDGNLNTDTIIRVNPDGSATPSFS
                     DGDNTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTENNENSAKAILDMAKGE
                     ITTHGTESYAAYANGTVVKAGDKLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGAGVVLESSINGQEATVDILSGSSL
                     RSANEILYNKNETSNVTITDSEVSSAADVFINNIKGHLTVDATDSKITGSANLSTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDFEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPIVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWLHNSKDFAVSMNGVKVEQDGARNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(9841..10176)
                     /locus_tag="OHO85_004075"
     CDS             complement(9841..10176)
                     /locus_tag="OHO85_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCQ2673376.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(10180..10524)
                     /locus_tag="OHO85_004076"
     CDS             complement(10180..10524)
                     /locus_tag="OHO85_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCQ2673377.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(10526..10699)
                     /locus_tag="OHO85_004077"
     CDS             complement(10526..10699)
                     /locus_tag="OHO85_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673378.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
BASE COUNT         3060 a         2189 c         2343 g         3111 t
ORIGIN      
        1 taaatatatt aaccgttata aaataacgta tagaaagtca agtgatcaca tttcaaatat
       61 caatttgata gtattggcct ggtgattatt tatgggtagc aataaaagga cagtatttat
      121 catccatagg gatagtctct gtacttttat tcccattatg ctaatgcctt actgaattat
      181 gaagcatttc tttagtatcc aactttagct agattaatgg tttattattt tctacatctt
      241 caatatataa aagcgtatta tcaatggcgt agtaactgcg tttgttatga ttaacatcag
      301 taacccaacg gaaaacgccc gcgcctgcta gtgttgaaca gtattcccga aatgtagatt
      361 ttccgcaaat atgaagcaat gcggcctctt ttattttagc agggttcttg gtcgtactaa
      421 cttttaacag gttcctggtt cctcttaata acaaaaccgt atcatcgtga gtaataattc
      481 tgatgttatc cgtagccaga taataaatgt aatgtgcaat acggtgatgt tttaattctg
      541 aataaaacca ggagaagttt tgctcttttc tcacttgctc aaacatcttt tgaaaaacaa
      601 cgacctgatc catcaggata ataacctctt gttagttgtg agactgcgta gtgtgcacga
      661 tcggttttac cacttcaatc tggtctgtcc tttggctgtg atatgtacag agtgtgatag
      721 agggaatatc tgaattctcc cggtgagcat tttgcaacgg accagctccg gtacaaacgc
      781 tgttgtgggt tcagattata acattctgtc taaggggcgg gataaaggtg aaattagggg
      841 gcatgaaaga tgactttata accttgctca ccccaatgtt gtaaaagttc gttttgcctt
      901 ctcgttggtg ccatgcctgt ccagacaatc aatgtttgcg tcgggaacag ttccgggcgt
      961 ggcgattcaa tgggatcagc aagaacagaa atgtgccatc ctgacaatga taaacgccat
     1021 gcttccagcc acaatcgtgc tctctcttca acattccacg ccatcagaat ggcttcttta
     1081 ccgggcttac ggcgcatttc gaaaagcgag gctgctgcgt attcaattaa tgcgccgtca
     1141 aacatactgc tcataatgcg ggaggtgttg tgatcaagca cgagacgctg gcgaacagga
     1201 aggtaaacat gattaatcaa ttgatctact gggtactctc gacccagtga aataattctc
     1261 gcgcgtagtt tggcaggatt agccatacga agaattgaca tcatctcttc ttgcaggcgg
     1321 ctccagtcat cttccgtatc ctggctggtg gtttccagta atgctttaac tttgcctaca
     1381 gggacgccat tacttatcca acgcttgatc tcttcgatgc gttgtatgtc ttcttcatca
     1441 aagagtcggt gtccgccttc actgcgctgt ggttttaaca aaccgtagcg gcgttgccag
     1501 gcccggagag tgacaggatt aatcccgcaa cgttcagcaa catcaccaat gctgtaataa
     1561 gccacaattc ctccttgcgg tcacaaatct ccgtcgcctg tacacgaccc aataatactt
     1621 tgtacaatat acgctaaaat tgtacaaagt ataaataaga tttcagctaa attggatgaa
     1681 acattatttt taatgtggat taaatttaaa cgtaacgtat tcattttcac gatgatttac
     1741 tgaaatcatg tgaaagaatg tgctgaaaat tattttttct ctggccacgc tatggaaggg
     1801 ataccattca atttagcttt agcaaacaga tctccctgaa acatctcaat tcctgcggat
     1861 tcaagccaca tccactcttc tggtgttgcc acgcccatag cactgacttg aatttcaagg
     1921 gatgtacagc attttatgat cgcctgaata attgcctgcc gtggcccact tttatgaaca
     1981 ttggtaatca attcctgact gattttaatt ctgtcaggct ggaagcgtga caggagtaac
     2041 aaaccagcaa aacctgcgcc aaaatgatca attgctacac tgataccagc agcctttagc
     2101 gatttaatcg cttcggcaaa ctcatcaaac cgagatatga cttcactttc agtaaattca
     2161 acgatgattt gttcaggcac cagagcattg gcctttattt cattaagtaa aaaagagact
     2221 gcgtcaggtt cgttaaccag ggtcataggt aatagattga ttgaaatcat tttatcaccg
     2281 agctcaagtg cgtgtgccat cgcgaatgca agcgccttac ttttgagatc cgctttgtag
     2341 atttccccgt ctttacgctg cccaaccgct atagctgatg ggctatcttc atttttttgc
     2401 acaatggctt caaaagcgat tatccgccgc gataagggat cgacaattgg atgaaaggca
     2461 aagtggtcgt ttatatttgg tgaaagggta tagaaatcaa gttctttatc agatccgtca
     2521 gcgataaaaa gccaggaatc ttcggcaggg atctcgaaat aggttgattg ttcggttgca
     2581 aggacaaaag tacgaaaaaa ttgtagcgct ctgtcatcat aagttagctg aaattttgat
     2641 gtgcctttgt cgaatacggc ctgtaaaacg tcatctcgct cgtgcaagcg caaatcaaat
     2701 aattccattc ccgctttgcc aaaacggcga gcaggcgcgt aatcgcacag cagttcaaca
     2761 atattatagt gccgtggatc ctgacatata gcccgatata tcattttaac ctgttcttcc
     2821 ggaccttcca gaagctggaa aaaatgagaa ccattaaaca gtaagatccc tgttacgtca
     2881 cactgcatgt tcctgcgatt tgctatcgaa accatttctt cgattttttt gacaggttcg
     2941 tcgtcacgta tatggctacg ataaataagg gtggtaagca ttaactgtcc agggtaatgg
     3001 gagaggtgag agtaagacgg taacagacat atcttccggt gtctttcttt taataccaaa
     3061 acataactgt ttctttacat tgataaaaaa tggaaaaagt taaagatgag ttggcgaaaa
     3121 tttttgtata ggttgtcatt taaatgatgc aataattttt atcataactt gttgttttat
     3181 atgtgttagc actaaatgca aaaaaataat ttatcattct gtacacatat ttcgtacaag
     3241 tttgctattg ttacctcact taacattgat taacattttt aacagaggcg tagcatgcat
     3301 caaaattcag tgactttaga ttctgcggga gcaatcactc gttactttgc aaaagctaac
     3361 ttgcctactc agcaggaaac tctcggggaa attgtgactg agattttgaa agatggacgt
     3421 aatctgagtc gaaagtcgct ttgtgccaaa cttctatgcc gactggaaca agcgacaggg
     3481 gaagaggaac agaaacatta taatgcacta attgggttgc tttttgaata atgcaacagc
     3541 ctgattattt aacaggttag ctatgaagtc gttatgaaga catctgataa tgaacgtata
     3601 aaatatgaaa ttactggcca ggcggtgctc cagatactgc gcataaagat aaatttttca
     3661 ttgcagactc ttattaagca attactcgta atgaaatcgg ctgaggaaga tgctttccga
     3721 cgcgatttaa ttgacagcat aattcgtgat tttagcaata gtgattcagg agggccaaac
     3781 cgaagaacag caacagctga caataaaagt atgttcaatg gtaagaaaat aaacagaata
     3841 cattaaaatt tcataagtaa gatgagaggt taccatgctt gaagatacta caattcataa
     3901 tgcaataact gataaagcgt tagcaagtta ctttcgcagt tcgggtaatt tgttagaaga
     3961 agagtcagct gtgttagggc aggctgtcac caatttaatg ctttcaggcg ataatgttaa
     4021 taataaaaat attatcttaa gtctgataca ctccctggaa acaacaagtg atatcctcaa
     4081 agctgatgtg attagaaaaa cactggaaat cgtgttgcga tacacagctg atgatatgta
     4141 acttctaatt attaagtata agtttataga aaactcattc atcttatttt tgtctgtcgc
     4201 tttagactat acacagcata attttattgg gttaatattt ctacgaggct gacatgaaca
     4261 attcaatccc agagagattt atttttcaat gtgctttgtt taaaaatctc gagagggagg
     4321 tgttcatgac gcatgggtat gttgatagtc atattattga tcaggcttta cgcttgcgtt
     4381 taaaagatga aaccagtgtg attctttctg atctctatct gcaaatcttg cagtacattg
     4441 aaatgcataa gacgacacta acggatatca ttattaatga cagggaatcc gtgctctctt
     4501 agaacttgag taaggttata gaataaacat cagctgtatc atcatgctgg tgcaaagtga
     4561 gcatttcagg cgttatgctt tcttaatata tccgcaatat cagttgtcaa gaatggagag
     4621 ttctcgttct ccattcttga tgcctgatat cccacctaat ttatttaatc ctgcctacat
     4681 tatgctcaat aattaatggg ttagatcaag atggagaaaa ttttatcttc ggcgtggata
     4741 tttcctatgc tctctaccat gctctctgta gatgccagcg cgagcgaatg aatggcattt
     4801 tcatgtataa aaccctttgg ccgggacaag caaatgcgca atacactcat acccatcctc
     4861 gttgcgatat gcttgtttat aaccggtgtc gctattttaa acatccagct ctggtattcc
     4921 gcaaaagcag agtacctggc gggagcgaga tatgccgcca acaatatcaa tcatatactt
     4981 gaagaagcgt cacaggcgac tcaaacagcg gttaacattg ccgggaagga atgcgacctc
     5041 gaggagcaat atcagcttgg cactgaagca gctctgaaac ctcacctgcg cacaatcatc
     5101 attctcaaac agggaatagt ctggtgtaca tccctgcctg ggaatcgggt cctgttgtct
     5161 cgtattcctg ttttcccgga cagtaattta ctgttggctc cggcaatcga caccgttaat
     5221 agattaccta tcctgctcta tcagagccaa tttgcagata cgcgcatttt ggttacgata
     5281 agtgatcagc atattcgcgg ggcgcttaat gtacccttga aaggggtaag gtatgtatta
     5341 cgcgtggcgg atgacattat tggacctacg ggtgatgtga tgacgcttaa tggacattat
     5401 ccctataccg agaaggttca ctccacaaaa tatcatttca ctattatctt taactcgccg
     5461 ccactcttta gcttctacag acttatcgat aaaggctttg ggattttgat ctttattctg
     5521 ttaatcgcct gcgccgctgc cttcctgctt gataggtatt tcaataaaag cgcaacgcct
     5581 gaagagatcc tgcgacgggc tataattaat ggggagatcg tcccttttta ccaacctgtg
     5641 gtaaatggtc tggaaggaac attgcgggga gttgaggtgt tagctcgctg gaaacaaccc
     5701 cacggtggat atatatcacc cgcggcattt attccacttg ctgaaaaatc cggattaatc
     5761 gttccgctta cgcaaagcct gatgaatcag gttgccagac agatgaacgc tatcgcgagt
     5821 aaactgccgg aaggttttca tattggaatt aattttagcg cctcgcatat tatttcgccg
     5881 acgtttgtcg acgagtgctt aaattaccgt gatagtttta cccgccgcga tttaaacctc
     5941 gttctggaag tcaccgagcg tgagccatta aatgttgatg aaagtctggt tcagcggttg
     6001 aacattctgc atgaaaatgg ttttgtcatc gcgctggatg atttcggtac tggctactca
     6061 gggctttctt atctgcatga cctgcatatt gattatatca aaattgatca cagtttcgtt
     6121 ggccgcgtaa acgcagaccc agaatcaacc cgaattctgg attgtgtatt ggatctggcg
     6181 cgtaaacttt cgatcagtat cgtcgctgaa ggtgtcgaaa cgaaagaaca acttgactat
     6241 ctgaaccaaa ataatatcac atttcagcag ggttattatt tctataaacc tgttacatac
     6301 atcgacctgg tcaaaattat cctttctaaa ccgaaggtga aggttgtggt tgagtgaaaa
     6361 ttgatcaata aggccatggt gcggtgtaat tatagacagc taattagctc gttgcctctt
     6421 gttactattg ttcattattt tgtttgctat aattgtttga aagttttgac aggattgcca
     6481 ttagtagcat gaacaatagt aataatctgg attatttcac tctctatatc atcttttcca
     6541 ttgcatttat gctgatcact ctcctggtca tccttattgc aaaacccagt accgggctgg
     6601 gagaagtgct tgtgacgata aatttgctta atgcccttgt ttggctggcg atcaatctgg
     6661 ttaatcgatt aagggaaaga ctcgtcagcc acagggatca gcaataatct ttcagtttct
     6721 cactgtcagt atgcggctga atggggtgct gacagtgaac gcctggatca ttgaaggaat
     6781 ggaattattg cgcaaatagt tgtcaaccca ggtgttatca cagttgtttt tatatatcat
     6841 tgaaataatt tctatggcaa ctatcaatga ttgtgatgtc gaagagttat ttgttaaaca
     6901 aaatcgtcac ctcaaagtga tcaatgtcat gaaaataagg tgaaaaatga taatgccgac
     6961 ttatttatca tttatatatt gtcgctgttt atctatttaa attttttgtg cttttgtttt
     7021 tgtctttacc ctccgttttg gtaatctgag ctcataggtt atttgaccag gaatgtttgt
     7081 tagtccaatg aatggatggt taatctatga aactgaaaaa actccccggg tttagtttag
     7141 gacttattgc tctggcggtg ggtaatgcat atgctgaaca gtttattcag cacgaggaac
     7201 ataaacatgc tgtggcaata aaagatggag aagtaaaaaa tggagaagta aaaggtggag
     7261 aagtaaaata tattgataat gcgctagtta taacaacggg taatggaagt tacggggtat
     7321 ccgttgcagg agtgggtagc aatttaacca ttaacaatgg cgtcattgta acaacgggcg
     7381 gtttatatcc ttctgacaat agttctctaa gcacagcatt aacagctagt gcaatcgtaa
     7441 gtgaagatgg cggtgctgtc actttgcggg gtaataatac gattgtcacg acgggagatt
     7501 attctgttgg tttgttaagt caagttgatg gaaacctgaa tactgatacc ataataagag
     7561 tcaaccctga cggttcggct accccctctt tttctgacgg agataatact tttattgtta
     7621 ctgcgggtaa tcacgcagtc ggcgttcttg cgtgtgcatc tcccggaagc gctcgtgcgt
     7681 gtgtatcttc tcttgatgaa gaaagtactg ccgatacaga aaataatgaa aatagcgcca
     7741 aagcaatact ggatatggca aaaggggaga taacaaccca cggaacagaa agttatgccg
     7801 cttacgctaa tggcactgtc gtcaaggctg gtgacaagct tgattatacc aatgccagcg
     7861 ttacattaac cgatgttgat atcaccacgc atggggacaa tgctcatgcc atcgctgctc
     7921 gccaggggac agtttcattt aaccagggag aaatttatac aacgggtcct gacgccgcga
     7981 ttgctaaaat ttataatggc ggcacggtga cgctgaaaaa tacatctgca gtcgcgcatc
     8041 agggggctgg agttgtgctg gagtcttcca taaatggtca ggaagcaacg gtagatattt
     8101 tatctggtag ctcactacgg tcagcaaatg aaatcctcta caataaaaat gagacgagta
     8161 acgtgaccat tactgatagt gaggtatcat cggctgcaga tgtttttatc aataatatta
     8221 aaggtcattt gaccgtcgat gcaactgatt caaaaataac gggttcagcc aatctttcaa
     8281 cagatgataa cactcatacc tatctgtcgc tttcagataa tagtacctgg gatattaaag
     8341 ctgactcaac ggtgagcaac ctcacggttg acaatagtac ggtctatatt tcccgggcag
     8401 atggaaggga tttcgaacca acaagattaa caataactga aaattacgtt ggtaataatg
     8461 gcgtattgca tctcagaact gaattgggtg atgataattc agctacggat aaagtcgtta
     8521 ttaatggaaa tacctctgga acaacccgag ttaaagttac caatgctggc ggcagcgggg
     8581 cttacacgtt aaatgggata gagattatca gcgttgaggg ggaatcaaat ggagaattta
     8641 ttaaggattc gaggattttc gccggtgcct acgaatattc attaacccga ggtaataccg
     8701 aagcgaccaa taaaaactgg tatctgacta acttccaggc aacgagcggc ggtgaaacaa
     8761 actccggagg aagttcagcg cctattgttg cgcctacccc cgtcctgcgc cccgaagctg
     8821 gaagttacgt cgccaacctg gcagccgcta acactctttt tgttatgcgt ctgaacgatc
     8881 gtgcaggtga aacgcgttac atcgatcctg taactgaaca ggagcgttca agccgacttt
     8941 ggctacgtca aattggcggg cataatgcct ggcgtgacag caacggacag ttgagaacga
     9001 cctcgcatcg ctacgtctcg cagttagggg ccgatctgtt aaccggtggt tttaccgaca
     9061 gtgacagttg gcgtttggga gtgatggctg gttatgcccg cgactacaac tcaactcatt
     9121 ccagcgtgtc ggattatcgt tcgaaaggga gtgtcagagg ctatagcgca gggctgtatg
     9181 ccacctggtt tgccgatgac atcagtaaaa aaggcgcata cattgacgcc tgggcgcaat
     9241 atagctggtt taaaaactcg gtgaaagggg atgaattagc ctatgaatcc tatagcgcga
     9301 aaggcgcaac cgtctcgctg gaagcgggtt acggctttgc cctgaataaa tcctttggtc
     9361 tggaagcggc gaaatatacg tggatcttcc agccacaggc acaggctatc tggatgggcg
     9421 tcgatcataa tgcgcacacg gaagccaatg gctcacgtat tgagaatgac gcaaataaca
     9481 acatccagac ccgacttggt ttccgcacct ttattcgtac tcaggagaaa aacagcggtc
     9541 cgcacggtga cgactttgaa ccttttgttg aaatgaactg gctccataac agtaaagatt
     9601 ttgctgtctc aatgaacggt gtgaaagtcg aacaagacgg ggcgcgtaat ttgggggaaa
     9661 ttaaacttgg cgtaaatggc aatctgaatc cggcggccag cgtctggggt aatgtgggcg
     9721 tgcagctggg tgataatggc tacaatgaca ccgcagtgat ggtgggcctg aaatataagt
     9781 tctgatcccc gttagctaaa ataccgcgtc gaattcatcg acgcggtaac atattaaata
     9841 ttattttttg ccgatagcac gcatggtgtc atcaattgcc gtgatcaaca gcatttgcgg
     9901 gtctttagcg caaacctgat tcagtttttc taccactttg gcgctcagtt ccggagattg
     9961 ctcaattttt aaatcacggc tggcaacgct ggcattaccc attaccgcaa caatttctgc
    10021 aacctgtgcg ctgtcagttt ttgccatttc gttggcttct gcgcaagtaa tataggtttc
    10081 tgacggcaaa ccgtttttaa tattgtagtc ctgcgcccag gtcattggtg cgaaaacaaa
    10141 caggcccgcc agtaaagcaa attttttcat catcattcct tatttcattt tacccagaat
    10201 tgcaccaccc gtaccgccaa tcacggcacc tttaatcgcc ccttcgaggc cattgccggt
    10261 cagaacgcca gtgacagcac caacggcggc acctactttt gcacctttac gcgcattttt
    10321 accgtcgcgg cctttttctg ttactgcacc aacaccagcg ccaacagctg cacctttcag
    10381 tacgccatta acaccattgc cagtaagtaa accaacgcct gcgcctagca atgcaccttt
    10441 cgtggtgcga ttcatatccg ccatcgctgg cgtggagcag aacaatgctg agataagccc
    10501 gaaggcaagt atttttttct tcaacttaga tgtccggtat taagtaagtt gcacacacaa
    10561 taatttcgtc ttcaattaag atctgcttaa ctaaagaacg ctcgctatta ttcagataat
    10621 tcaaaatgag cgtggctgtg atgataggaa ttatgttttt tacgtgaatg agaataatct
    10681 taaatgagga ataactcatt gat
//
LOCUS       DAJOWB010000143        10700 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJOWB010000143 DAJOWB010000000
VERSION     DAJOWB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.143"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            37..750
                     /locus_tag="OHO85_004078"
     CDS             37..750
                     /locus_tag="OHO85_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCQ2673379.1"
                     /translation="MNQRNMSIINSTPVHVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEILQASLRAIHAALS"
     gene            782..1540
                     /locus_tag="OHO85_004079"
     CDS             782..1540
                     /locus_tag="OHO85_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673380.1"
                     /translation="MDALQTQTVISTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1586..2935
                     /locus_tag="OHO85_004080"
     CDS             1586..2935
                     /locus_tag="OHO85_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCQ2673381.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPHQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFRLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            2935..3759
                     /locus_tag="OHO85_004081"
     CDS             2935..3759
                     /locus_tag="OHO85_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673382.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            3771..4331
                     /locus_tag="OHO85_004082"
     CDS             3771..4331
                     /locus_tag="OHO85_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCQ2673383.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4362..5432
                     /gene="lptF"
                     /locus_tag="OHO85_004083"
     CDS             4362..5432
                     /gene="lptF"
                     /locus_tag="OHO85_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCQ2673384.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLVQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            5429..6508
                     /gene="lptG"
                     /locus_tag="OHO85_004084"
     CDS             5429..6508
                     /gene="lptG"
                     /locus_tag="OHO85_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCQ2673385.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(6544..7716)
                     /locus_tag="OHO85_004085"
     CDS             complement(6544..7716)
                     /locus_tag="OHO85_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCQ2673386.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(7716..7964)
                     /locus_tag="OHO85_004086"
     CDS             complement(7716..7964)
                     /locus_tag="OHO85_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCQ2673387.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(7996..8910)
                     /locus_tag="OHO85_004087"
     CDS             complement(7996..8910)
                     /locus_tag="OHO85_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCQ2673388.1"
                     /translation="MNQTVAVTGATGFIGKYIINNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTHCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVPGGYDW
                     QHVRQLAADARCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(8907..10637)
                     /locus_tag="OHO85_004088"
     CDS             complement(8907..10637)
                     /locus_tag="OHO85_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCQ2673389.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHDNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPG
                     DRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIELLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLHVQESLA"
BASE COUNT         2568 a         2665 c         2809 g         2658 t
ORIGIN      
        1 gtaatattca atacattatt tactgccggg tttttcgtga atcaacgtaa tatgtcaata
       61 attaattcca cgccagtgca tgttattgcc attgtaggat gtgatggttc aggtaaatcg
      121 accctcacgg caagcctggt aaatgaactg gcagcaagaa tgccaacaga acacatttat
      181 ctcgggcaat cgtccgggcg aattggcgaa tggatttcac agctccctgt tattggcgca
      241 ccttttgggc gttatctgcg aagtaaagcg gcacatgtgc acgaaaagcc ctcaacaccg
      301 cctggcaata ttactgcact ggttatctat ctgctttcct gctggcgggc gtacaagttt
      361 cgcaaaatgt tgtgtaaaag ccagcaaggc tttctgctca tcaccgaccg ctacccgcag
      421 gttgaagtgc cggggtttcg ctttgatggc ccgcaattgg caaaaaccac gggcggtaac
      481 ggttggataa aaatgttaag gcagcgcgag ctgaagctgt accaatggat ggcatcttat
      541 ttgcccgtat tgttgattcg tcttggcatt gatgaacaaa cggcgtttgc gcgtaaacct
      601 gaccaccaac tggcagcgtt acaggaaaaa atcgccgtta cgccgcaact gacattcaat
      661 ggtgcaaaga tccttgaact ggatggtcgg caaccagccg atgaaattct gcaagcgtca
      721 ctacgcgcaa ttcacgccgc gctttcctga tcaccaaaat ggatagggga tgatttaacg
      781 aatggatgca ctacaaacac aaactgttat tagtacaacc gcgccgcagc ccagctacat
      841 tccggggcta attgcggtgg tcgggtgtga tggcaccggt aaatccacac tgaccaccga
      901 cctggtgaaa tcgctgcaac aacactggca aaccgagcgg cgctatctgg ggctgctctc
      961 cggcgaagac ggcgacaaaa tcaaacgatt gccgttggtt ggcgtctggc tggaacggcg
     1021 actggcggcc aaatcctcga aaacccaaag catgaaaacc aaatctccgg cgctatgggc
     1081 ggcggtgatt atgtactgct tctcgctgcg aagaatggcg aatctacgca aggttcagcg
     1141 actggcgcaa agtggcgttc tggtggtcag cgatcgcttc ccgcaggctg aaatttcggg
     1201 cttttattat gacggaccgg ggattggcgt cgaacgtgcg accgggaaaa tcagcatgtt
     1261 tctggcgcag cgcgaacggc gtttatacca acaaatggcg caatatcgcc cggaattaat
     1321 tattcgcctg ggcattgata ttgagactgc catctcccgc aagcctgacc atgactatgc
     1381 cgagctgcag gacaaaatcg gtgtcatgtc gaagattggc tataacggca caaaaattct
     1441 tgagattgat tccagagcgc cctacagcga agtgctggaa caggcacaga aggcggtttc
     1501 tctggttgcc atcgtttctg accgccgaag tttaacttaa ggctggcggg atacttttta
     1561 ctatcaacga taaggtcagg ctgaattggc gggttttaac atcaaacatt ggtttgcaga
     1621 tggcgcgttt cgcaccatta ttcgcaatag cgcctggtta ggctccagta atgtcgtgag
     1681 cgccttgctg gggctgttgg cgctctcgtg tgccggtaaa gggatgacgc ccgccatgtt
     1741 tggcgtactg gtgattgtgc aatcgtacgc caagtcgatc agcgatttta ttaagtttca
     1801 gacatggcag ctggtggttc agtacggaac gccagccttg accaacaata atccgcacca
     1861 atttcgcaat gtcgtctcat tttccttctc gctggatatc gtcagcggcg cggtggcgat
     1921 tgtcggtggc attgccttac tgccattcct ttcccattca ttaggtctgg atgaccaaag
     1981 ttttcggctg gcagcgctct attgcacgct cattccttca atggcttcct ccacgccgac
     2041 cggcattctg cgtgcggtag atcgcttcga tttaattgct gtacagcagg cgacgaaacc
     2101 ttttctgcgc gcagcgggga gcgtcgtagc ctggtatttt gactttggtt ttgcgggttt
     2161 tgttattgcc tggtacgtgt cgaatctggt tggcggcacc atgtactggt ggtttgccgc
     2221 gcgcgaatta cgccgccgaa atatccataa tgccttcaaa ttgaatctgt ttgagtctgc
     2281 ccgacacatc aaaggcgcgt ggagttttgt ctggtcaacc aacattgccc actccatctg
     2341 gtcggcgcgt aactcgtgca gcacagtgct ggtggggatc gtgttaggac ccgctgccgc
     2401 cgggttattt aaaatcgcca tgacattctt cgacgccgcc ggaacgccag cgggtttgct
     2461 gggtaaaagt ttttacccag aggtgatgcg tttagatccg cgcaccacca gaccgtggtt
     2521 gttaggtgtg aagtccggtt tactggcggg cggaatcggt attctggtgg cgctcgcggt
     2581 gtttatcgtc ggcaagccgc tcatttcgct ggtgtttggc gttaagtatc tcgaagcgta
     2641 tgacctgatt caggtgatgc tgggcgcaat tgtgatttcg atgctgggct tccctcagga
     2701 gtcattgcta ttgatggcgg gaaaacagcg cgcttttctc gtggcgcaaa ccatcgcctc
     2761 aatcggctac atcgtattgc tgttcatgtt ctgtcatctg tttggtgtgc tgggagcagc
     2821 gtttgcttac ttcggtgggc aatgtctgga cgtggcgctc tcgttaattc caaccttaaa
     2881 ggcatttttt cagcgccatt ccttgcttta taacgcagcc ggagagaaat cctgatgatg
     2941 agaaaatatt tcccgcttga ggcgagcgag cgtctatttg ttgcgattga agaagatgat
     3001 gtcgttgatg cacaggttag tttgccgccg actattgcac taagttgtac caccgaaatc
     3061 attcatgata attacgcgtt atgcctgcaa ttctggctca atggcgttga tcgtcaggaa
     3121 ttactgcgtt tagttcgcaa acaagccaaa ggcgatgagc tgactgctga tgagcgcaaa
     3181 caatttaaat acatgcgagc gcgctacaag cacctgcgtt ttgcgcaacg gctgtattta
     3241 aagaagcatc aggccggatt tttattcggc aaaacgaccg tttttcttgg gcgttttcag
     3301 gacggctttc gtaacggtaa gaaaaatatt gtgtcgtatt acggcaacct gttacggatt
     3361 tatttaagct ctccggtctg gtcgttggtg aactattcat tacgccatag ccagttagaa
     3421 agcgtaagca gttttattgc ctatcggcaa aagcaaatgc ataccctgaa agagattatt
     3481 gccaaaccgc gactaaccgg cagggaattt catgatgtgc gaaaaatcat cagccaacag
     3541 gtttcttatt acgacacctt aagatcgctt gatcctgaaa ataaagaggc gttacagatt
     3601 tcccgtttcc tcgccgccat taatggcctg atgggagata aacacgacga catggtggcg
     3661 gatgatatgg aaaaccgcca gtcgtatgat gcaccgttgg cgctggatag cgatattcgt
     3721 cagcgcttag agctgctgat ttcgcgtttt ccgctataac cggagcaatg atgtcatctc
     3781 gtttgattat tgcgctgatt atcatgcttt tagccccggg tgtgcaggca cacaatttcg
     3841 tcactggcaa aacggttacc cctgtttata ttcaggaagg cggcgagctg cttctgaata
     3901 gcgacgacga aattcactac caaaagtgga acagcacaca attggcggga aaagtccgca
     3961 ttatccagta tatcgccggg cgtaagtcgg cgaagaaaaa gaactcactg ttaattaaag
     4021 cggttgaagc ggcaaacttt ccgcaggatc gttttcagcc caccaccatt gtgaacaccg
     4081 atgatgccat ctttggtact ggctattttg ttgttggcaa aatcgaaaaa aataaacgcc
     4141 gctatccgtg ggcgcaattc gttattgacg gcaatggaca gggacgcgtt gcctggcgct
     4201 tgccggagca gagttcaacc attctggtgc tgaataaagc cgggcaaatt cagtgggcaa
     4261 aggacggcag cctgacgccg gaagaggtag atcatgtcat tgcgctggcg cagaaactga
     4321 tcaatgaata aaaaattcaa aggattgagg gcgttagtga gatgaagctg gttgaacact
     4381 acatcatgcg tggtacgcgc cgtctggtgc tgattatcgt cggttttctg atctttattt
     4441 tcgccagcta ctccgcacag cgttatctga ccgaagcggc aaatgggaca ttagctcttg
     4501 atgttgtgct ggatatcgtt ttctacaaag tgctgattgc actggagatg ttgttacctg
     4561 ttggactgta tgtgtcagtt ggcgtgacgc tagggcagat gtataccgat tcggaaatta
     4621 ccgctatctc tgcggcgggg ggcagtccgg gacgcttgta caaagccgtt ctttatctgg
     4681 cgataccgct aagtattttt gtcacccttc tgtcgatgta tggtcgtccg tgggcttatg
     4741 cgcagattta tcaactggag cagcagtcgc agtcggagct ggatgttcgc cagttgcggg
     4801 caaagaaatt taacactaac gataacggac gaatgatcct ttcgcagacg gttgatcagg
     4861 ataataatcg cctgactgac gcgctgattt atacttctac tgccaatcga acccgcattt
     4921 tccgcgcccg ttcggttgat gtggttgacc catcacccga gaaaccgacc gttatgttgc
     4981 acaacggcac tgcctatctt ctcgatcatc aggggcgtga cgacaacgaa cagatctacc
     5041 gtaatctgca attacatctg aatccgctgg ttcaaagccc taacgtcaaa cgcaaagcga
     5101 aatcggtcac ggagctggcg cgctccgtct ttcctgccga tcatgccgaa ctgcaatggc
     5161 gacaaagccg tggtctgaca gcattgttga tggcgctgct ggccatttca ttaagtcggg
     5221 taaaaccgcg gcaagggcga ttttcaacgt tattgccact gacgttgctg tttattgcca
     5281 ttttttatgg cggcgacgtc tgccgtacgc tggtggctaa cggcgcgatt cccctcattc
     5341 ctggtttgtg gttagtgccc ggactcatgc taatgggcct gctgatgctg gtcgcacgcg
     5401 acttctcttt gctgcagaaa ttttcccgat gaatgttttc agtcgctatt taatccgtca
     5461 tctctttctc ggttttgccg ccgccgcagg gctattgctg ccgcttttta ccaccttcaa
     5521 cttgattaac gaactggatg atgtcagccc gggcggttat cgctggactc aggcggtgct
     5581 ggtggtgcta atgaccttac cgcgcacact ggtcgaactt tcgccgttta tcgcgttatt
     5641 gggtggtatt gttggactgg ggcagttatc gaaaaacagt gagcttaccg ccattcgcag
     5701 cacggggttt tctatcttcc gtattgcact ggtggcgctg gttgccggga tattgtggac
     5761 tgtttcgtta ggcgcgatag atgagtgggt ggcgtcgcca ttgcagcaac aggcgttgca
     5821 aatcaaatcg accgccaccg cgttggggga ggacgatgac attaccggca atatgctgtg
     5881 ggccaggcgt ggtaatgaat ttgtgacggt gaaatcgctg aacgagcagg gccagccggt
     5941 gggcgtggag atatttcatt atcgcgacga tctctcgctt gaatcctaca tttatgcacg
     6001 cagtgccacc attaaagacg acaaaacgtg ggtcctgcat ggtgtgaatc ataaaaaatg
     6061 gctgaatggc aaagaaacgc tggaaacatt agataatctt gcctggcaat cggccttcac
     6121 cagtatgaat cttgaagagt tatcaatgcc ggggaatact ttttctgtcc gtcagcttaa
     6181 tcattacatc cattatttgc aggaaaccgg gcaacccagc agcgaatacc gccttgcact
     6241 gtgggaaaaa ctggggcaac ccatcctgac cctggcgatg attttgctgg ctgtgccgtt
     6301 tacctttact gccccgcgtt cgcctgggat gggtagccgt ctcgctgtag gcgtcatcgt
     6361 gggcttactc acctggatca gctatcaaat catggttaat ctgggattgc tatttgcgtt
     6421 aagcgcacct gttaccgcgc tcggtttacc ggtagcgttt gtgttggtgg cgttgagcct
     6481 ggtgtattgg tatgacagac aacattaaac ccgccgtggc gcaaacacca cggcgggctc
     6541 acattactgc ttcagagtag caaatgcctg aatgatggca tcgatttgct cacgactgtg
     6601 ggcagcattt acgctacagc gcagcagcgt gatgcctgca ggcgcggcag gcggcaggat
     6661 aagattcaca tacacgccca gcgagataag ctcgcgccag atgcgtaaac cttcctcttt
     6721 cgaaccgatt attactggca ctaccgggct aatatgtgaa cttaactcat aacccagttt
     6781 ttgcagaccg tcatacagat ggttggcgtt atccatcagc ttttgccgca gctctggttg
     6841 tgtggcaata gttcttaacg acgagcgcac ggtcgcaatg caggacggag aaggagaggc
     6901 ggtaaaaata tacgggcggc tgccgtagcg cagcacttcc atcgcttccg aaccgacagc
     6961 aaaaccgcca atcgaggcca gacttttact gaaagtacca acgataatat ccacatcatc
     7021 ttcaacgccg acagcttcag ccagtccgcg ccccgttgcg cctaacacgc caaacgaatg
     7081 cgcttcatcg acaatcaaat aaccaccgag acgacgtttg atatcaacaa tttccgccag
     7141 cggcgcaacg tcaccgagca tgctgtagat gccctcaacg atgataatcg cctctttcgc
     7201 ccgttcaccg aggcgaacca tgcgccgttc cagatcttta gcatcgttat ggcggaaacg
     7261 aataatttcc gcgccaccga gagaacaggc gtcataaata ctggcgtggc tgtcggcatc
     7321 cagtaacacc acggcgttat ggtcggcaag tgcgctgatg acgcctaaat tagcggtata
     7381 accggttgaa aaaacgatag cggtagggcg attgaaaaac tcggcaatct ctttctctaa
     7441 cgccaggtgc ggcgcataac tgccgtttgc catacgagaa ccggtggtgc cggtgccttg
     7501 ggccgctaac gctgcctgac cttcgctgat ggcatcatga ttaaaggtta aaccgagata
     7561 attattggtc ccggccagaa tcactttttt attgccgatc cggccttcgg tggcggaata
     7621 cacttcatca atacaggtgc caaacggtgt taacccgttg tcactaaatt gcagtcgctc
     7681 gccagcaagg cgcgcatatt tatcgtatag ccccattatt tattctccag ccatggaagt
     7741 agagtttcca ttaattgcgc aggggttttg acatccagca gaatattaat aggaatggaa
     7801 atatcgaatc tgtcttccag catcattaag agatccatca ctttgatcga ctccaggcca
     7861 agatcgttga cgagatcgct gtcgggctta atttccacac cgttttcgac caaatcctgt
     7921 aaacaggaaa ggatgtaatc cattactatt tcacgattta ccatagaaac gttaacacac
     7981 ctttaaacaa gacaatcaaa atagcccgtt gcgtaatgcg tgttccaggc taatcccggg
     8041 aaaccaatta atatcttcag aaatacggtt attacttgcc gaccagtcgg cgtgggttaa
     8101 ttcacgaatt ttcgagcggg tcagcatagg ttctttacct gccaggcgac tcaacgcggt
     8161 actgatatcc gcaaggcagg tgagtaccgg cagaggaatg ccaaccattc gcacggaacc
     8221 acaacgggcg tcggcggcaa gctgtcgtac gtgttgccag tcatagccgc cggggacgcc
     8281 atcgcataat tcataggttt gcgtctgtac agtttcggcg cttaaccact gacccactgc
     8341 ttgcgcgaaa tcggtgacgt gcaggaaaga gagctgtgta tctggcgcac caagtcgtgg
     8401 cagcaggccg cgcagcatcc agtcaaacag cggttttaac tctttatcgc cgggaccata
     8461 cacggctgtc gggcgaaaaa cgcccagcgt aatttcgtca gccattgcag tcagacgttg
     8521 ttcggcgacg tgtttggaat ttgcgtacca ggagagctcg ggatggcgcg ccgccagcga
     8581 agagataaac agaaaacgtt ggcaaaagcc gctctctttt gctgcttgca tcaggcgcag
     8641 gctgccgtca acgttacagt gggtaaaaat ctcttctttg tgcccgcgca cttgcccggc
     8701 gcaatggacc accgcgctgg ctccggcaac cagcttgcta agtgaatgag tatcttccag
     8761 cgaaccgcgc acccaggtga gattatcatt gacgtgagcg cgggcagtac gcgtcaatgc
     8821 gcgaacgtga aagccgcggg cgagcaggtt attgataata tatttaccga taaacccggt
     8881 agcgcccgtc accgcgacag tttgattcat gccagggatt cctgcacatg aagactggca
     8941 gcataagcct tctgataacg ttttttcgct tccgcacggg caggcttgcc ggaggacgtt
     9001 cggggaatac tgtggggcgg caacagctcg atagccgcgg tcacgccaaa ttcgctttgg
     9061 atccgtgccg ccagcgcgtg gataagctgc ccgcgacgtt cttcgtcgct gatccgacac
     9121 tggatctgca aaatgatttt ttcctgggcg gtaacaaaag caatcgcatc gccagaatga
     9181 atttccggtt cttgttccgc aatatattca atatcctgcg gccagatatt acggccacga
     9241 ataataatca gatctttaat gcgtccggtg acatacagat aaccgtccag cagataaccg
     9301 aggtcgccgg tgtctaacca gcccgtcgcg gcaatctcgt cttgcgaagc ctggtcgcca
     9361 aagtaaccgc tcatcagact gggaccggag atgcaaatat ggcctaccac acgttccgcg
     9421 accggcatac ctgcttcatt gcggatttca ataccatgtt ccggcaacgc tttgccgcag
     9481 ttgacgaaag tcgatacggc gcgtgtctct gcacccggcg cgacggcttt accctgatat
     9541 tcgaggatgt cgcgatccac ttcgttaacc acaaccccgg aggcttcatc agagaagctg
     9601 acagccagcg cattttctgc cagtccgtag cacggcatga aagttttatt gtcaaagtta
     9661 acctgacgga aacattcagc gaattgatgg agttgttctg cggagatggg ttctgcacca
     9721 ataccagcga cgcgccagca ggaaagatcc agttcagcga gatctttttc attcacgcgg
     9781 cgctggcaca attcatagcc aaacggcggc gcaacggaaa cggtgccgcg atttttactg
     9841 atcaatttaa gccattgcag aggacgcatg gcaaaatcct gagtgcgcaa ataatctact
     9901 gaaagctgcg tggcgacggg ggtcaggaga aagccgacca gtcccatatc atggtagaaa
     9961 ggcagccagg agacgcagcg gtcgccaggg cgtaatttaa tgccgtcgtg gcttatagca
    10021 cgtagattag ccatcacttc gcgatgggtg ataatgacgc cacggggaaa acgggtgctg
    10081 ccggaggtgt actggaggta ggcgatatcg ttcggaactg gacgctggag cgcaacatcg
    10141 gcttccggta atgccttaaa ccaggcgtgg cttaaaacat gtaattcggg gttgtcatgc
    10201 gtcgcggcat tgaccagtgg caaccactca tcaccagtga taatggctgc gggctggcaa
    10261 cttgccagta aaccctgcaa tttggcgctc caggaatccc gctgaccaac gcccattgga
    10321 atcgccaacg ggacggcgac taagccggca tactggcagg caaaaaaagc ctctacgaac
    10381 ccgctacttg tttcggcaat cagtgccacg cgatcgcctt ttttcaggtt cagcgataac
    10441 aaccgcttcg caccagcttc ggcacgcgtt tttaatgtct gatattccag ttgatcttca
    10501 agttggcaac gtctgtcata aaaattcatt ccggcgctac tcagagcggc gtagtccaaa
    10561 gcatcaacca gcgttggaaa atcggcatag cgcataggta gggaatgcgt aaagatttta
    10621 ttagacatat acaccacgaa aaaattggat atcaaaaata aaatgacaat aatgcggtgg
    10681 attatatagg cataaaagat
//
LOCUS       DAJOWB010000144        10416 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJOWB010000144 DAJOWB010000000
VERSION     DAJOWB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.144"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            34..414
                     /gene="crcB"
                     /locus_tag="OHO85_004089"
     CDS             34..414
                     /gene="crcB"
                     /locus_tag="OHO85_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCQ2673390.1"
                     /translation="MIKSLFAVIIGGSVGCTLRWLLSTRFNSLFPNLPPGTLVVNLLA
                     GLIIGTALAYFLRQPHLDPFWKLMITTGLCGGLSTFSTFSVEVFALLQAGNYIWALTS
                     VLVHVIGSLIMTALGFFIITILFA"
     gene            complement(794..1987)
                     /locus_tag="OHO85_004090"
     CDS             complement(794..1987)
                     /locus_tag="OHO85_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2673391.1"
                     /translation="MSSKIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISLTGSKTVVLWALGGYGVSFTLLGLGSVLM
                     ATGAVTTAVGLGILIIARIVYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLVIHPLAPVWMLMAAPVWAVVMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAMLLAAAMSMMQLGLSPALTRQFDTDTTTISQQVAWLLGLSAIAALIAQFVV
                     LRPQRLTPVALLLSAGVLMSSGLAIMLTEQLWLFYLGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLAASHTLGYGLCALLVPLASKTGVSIALIVTALFAAVLFTIVSACIWH
                     YRTIK"
     gene            2123..3847
                     /gene="iucA"
                     /locus_tag="OHO85_004091"
     CDS             2123..3847
                     /gene="iucA"
                     /locus_tag="OHO85_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCQ2673392.1"
                     /translation="MILPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPAHLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESYAHTQQTIDARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RQEAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQVWCQALFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSVRTLSVKEVERGMRLARLAQTDGWQMLQARFPTFRVMQEDGWAGLRDL
                     NGNIMQESLFSLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATEWLDTIDEAQAENIFTREQLLRYFPYYLLVNSTFAVTAALGAAGLDSEANLM
                     ARVRTLLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3848..4795
                     /gene="iucB"
                     /locus_tag="OHO85_004092"
     CDS             3848..4795
                     /gene="iucB"
                     /locus_tag="OHO85_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCQ2673393.1"
                     /translation="MSGANIVHSGYGLRCEKLDKPLNLGWGLDNSAVLHWPGELPTGW
                     LCDALDQIFIAAPQLSAVVLPWSEWCEEPQALTLFGQVQSDIIHRSAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQIAYLERQLTSKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGVTHYLLLNEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4795..6537
                     /gene="iucC"
                     /locus_tag="OHO85_004093"
     CDS             4795..6537
                     /gene="iucC"
                     /locus_tag="OHO85_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCQ2673394.1"
                     /translation="MNHKDWDLVNRRLVAKMLSELEYEQVFHAESQGDDRYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLNADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNEMDIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            6534..7811
                     /gene="iucD"
                     /locus_tag="OHO85_004094"
     CDS             6534..7811
                     /gene="iucD"
                     /locus_tag="OHO85_004094"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCQ2673395.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELDCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKRRLFLVQTSQGEYFARNICLGTGKQPYLPPCVK
                     HMTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLEEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFVVNASMQTHGIAEPQLSL
                     MAWRSARILNRVMGRDLFDLSMPPALIQWRSGT"
     gene            7893..10094
                     /gene="iutA"
                     /locus_tag="OHO85_004095"
     CDS             7893..10094
                     /gene="iutA"
                     /locus_tag="OHO85_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCQ2673396.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAAFLQSGYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2485 a         2726 c         2702 g         2503 t
ORIGIN      
        1 tgttacgata aaaaattatt ttatgagggg tacatgatta aatcactttt tgccgtcatt
       61 atcggcggtt ctgttggatg tactctgcgc tggttactgt ccacacgctt taacagtctt
      121 tttccaaatc tacctccagg tacactggtg gtgaacctcc ttgccgggtt aattattggt
      181 actgcgttgg cgtacttttt acgacaacca caccttgatc ctttttggaa attaatgata
      241 acaacgggac tttgtggagg tctgagtact ttctctacat tttctgttga agtattcgca
      301 ttactccagg cgggaaatta tatatgggct ttgacgtctg tacttgttca cgtcattggc
      361 tccctcatca tgacagcttt aggctttttt atcataacca tacttttcgc atgagaattc
      421 attaacgtta caggtttatc aactaatgtg acgcttagga tagaatttcg ctccatggta
      481 tgcgtttgca gtcattacag tctccatgac gactgtaatg actgcaaacg cattacctga
      541 catatcagta taaacaatga attaacctct tggcgtattt tttacatcac tggccctcac
      601 cccccaaaat tctctctgaa aaattgttgc ggttaacgtt gccaatgtat ccacccattt
      661 cagaagacga tctggcatcc tggtggcatg ctgcgatgtg cttcaggtga gtgcactggg
      721 cggtcagcac aggcggtttc gacagcgaaa ttgcacagga cgttatgccg gctgtgagta
      781 caacatcata gccttacttg attgtacggt aatgccaaat acatgcagac actatggtaa
      841 ataatacagc ggcaaataat gccgtcacaa tcagcgcgat tgagacacct gttttcgacg
      901 ccagtggcac caacaatgcg cacagcccat agccaagtgt gtgactggcc gcaagccagc
      961 ccgcgcctgc gccgtcggcc agcttatcat tcagtaaaag ttgataagcg ggtgttgcca
     1021 gagcggcccc aaatgacagc actgcacagc ccaggtaaaa caaccatagc tgttcagtga
     1081 gcatgatagc cagaccacta ctcatcaaca ccccggcact caggagcaga gccactggag
     1141 tcaggcgctg tggacggagt accacgaact gcgcgataag cgcagctatt gcggacagcc
     1201 ccaacaacca cgccacctgt tggctaatag tggtggtatc agtatcgaac tggcgagtaa
     1261 gggctggcga aagtccaagc tgcatcatgc tcattgccgc agccagtaac attgcgcaaa
     1321 gcagataagg caggcaatcc cgcttcaggc tgacgctctt gcgctccggt gtgggctgtg
     1381 gtggtgtgcc gggcaaccgc agaagcatca ccacagccca caccggagct gccatcagca
     1441 tccacactgg cgccagaggg tgaatgacca acattgccgc cgcgcacagc ggcccgaata
     1501 gcctgccgca gctcaggccg gagctgatgg ttgccagagc ggccatgcga ttcccttctc
     1561 ccgctctctg caatgcccag acctgacagg ctggcaccat tgctgacacg gtcagaccgt
     1621 agacgatccg ggcgatgatt aatattccca accccaccgc tgttgttacg gcaccggtag
     1681 ccatcagcac gcttcccagc ccaagcaggg taaagcttac gccatatcct cccagagccc
     1741 acagcaccac agtcttactg ccagtaaggg aaatttgctt tccccaccat ggggaagatg
     1801 gcagaaacag cattgagccc agcatcagca gggcagccca tacagacaga ctcagatttg
     1861 tctgtatgac cagaacaggg agtacaacca gcagaccgtt ctgtccgata ccgagaagac
     1921 cggcactgaa cgccagtggc cagcaggaca gggttttttg gggcgtatct tcgatcttag
     1981 atgacattgt aatgtttcaa caccatgtat taattgtgtt tatttgtaaa attaattttt
     2041 ctgacaataa catttctcat tgataatgag aatcattatt gacataattg ttattatttt
     2101 actgtgtagg agctgtttga ttatgatcct gccctctgaa aaatccgcca cagatgtggc
     2161 tgcgcagtgc ttcctgaatg ccttgattcg tgaaaccaaa gactggcaac tggcggaata
     2221 cccgccagac gaattgatta tcccgctgga cgaacagaag tcgctccatt tcagagtggc
     2281 ttatttctcc ccaacccagc atcaccgctt tgcatttcct gctcatctgg tcacggcatc
     2341 aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata agctccggca
     2401 tcaacttttt ctgccagttc ccctctgcga aactttccac caacgcgtgc tggaaagcta
     2461 cgcccatacg caacagacaa ttgatgcccg ccatgactgg gccatcctgc gtgaaaaagc
     2521 gttgaatttt ggcgaggctg agcaggcact gctgacagga cacgctttcc accctgcacc
     2581 taagtctcat gaaccgttta accgccagga agctgaacga tacctgcctg acatggcacc
     2641 tcacttcccg ctgcggtggt tttcggtgga taaaacgcaa atcgctggtg aaagtctgca
     2701 tcttaacctt caacagcggt tgacgcgatt tgccgcagaa aatgcgcccc agttactcaa
     2761 cgaattaagt gacaatcaat ggctgttccc gctgcacccg tggcagggag aatatctttt
     2821 gcagcaagtg tggtgccagg cactttttgc taaaggactt atcagagact taggcgaggc
     2881 cggcacgtcg tggctgccga ccacctcttc ccgctccctc tactgtgcta ccagccgcga
     2941 tatgatcaag ttctccctga gcgttcggct gaccaactcc gtccgtactc tgtctgtgaa
     3001 agaggtggag cgaggaatgc gcctggcacg tctggcgcaa accgacggct ggcagatgct
     3061 acaggcccgc ttccctactt tccgggtaat gcaggaggac ggctgggccg ggctgcgcga
     3121 tcttaacggc aacatcatgc aggaaagttt gttttccctg cgtgaaaacc tgctgctgga
     3181 gcagccgcaa agccagacca acgttctggt ttccctgact caggccgcac cggatggcgg
     3241 tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta tcactgtgca
     3301 acaggccgcc cacgcatggg tcgatgctta ctgtcagcag gtgctgaagc ctctgtttac
     3361 ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc ttgtgcagat
     3421 gcttggggat ctgccggtcg gatttattta ccgtgactgc cagggcagcg cgtttatgcc
     3481 tcacgcgaca gagtggctcg ataccattga cgaggcgcag gcggaaaata ttttcacccg
     3541 tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt ttgccgtaac
     3601 tgctgcgctg ggtgctgccg ggctggacag cgaagcgaat ctgatggctc gtgtacgaac
     3661 attgctggct gaagtgcgtg accaggtgac tcataaaacc tgtctcaact atgttctgga
     3721 aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc ataacgagaa
     3781 caccatcgtt gacccttcgg tgatctattt tgatttcgct aacccgctgc aggctcagga
     3841 ggtctgaatg tctggggcaa acattgttca cagcggatat ggactgcgct gtgaaaaact
     3901 cgacaagcct ctgaatcttg gctgggggct ggacaatagc gcggtgttgc actggcccgg
     3961 ggagctgcca acagggtggc tgtgcgacgc gctggatcag atatttatcg ccgcaccaca
     4021 actttcagca gtggttcttc cctggtccga atggtgtgag gagccacagg cgctgacgct
     4081 tttcggacag gtacaaagcg acattatcca tcgttccgct ttctggcagt taccgttatg
     4141 gctgagttct ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag agcgtgagat
     4201 ttatttcccg cagcgccccc cccgtccgca gggtgaagtt tatcgtcgtt acgatccacg
     4261 cattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag aacgtttcac
     4321 tcgctggatg aacgatccgc gcgttgagta tttctgggag caaagtggct cactggaggt
     4381 acagatcgcc tatctggaac gccagctcac cagtaaacat gcattcccgc tgattggttg
     4441 tttcgacgat cggccgttca gttatttcga aatctactgg gcggcggaag accgcattgg
     4501 tcgccactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg gtgaacagca
     4561 atggcgcgga gcccactatg tgcaaagctg gctgcgcggg gtgacacatt acctgctgct
     4621 gaatgagcca cgtacgcagc gcacagtact ggagccacgc accgataacc agcgcctgtt
     4681 ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc cacataaacg
     4741 ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc tgtaatgaat
     4801 cacaaagact gggatttggt caaccgccgc ctggtggcaa aaatgttgtc tgagctggag
     4861 tatgagcagg ttttccacgc ggaatctcag ggcgatgacc gctactgcat taacctgccg
     4921 ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctctg gattgatgct
     4981 caaactctgc gctgcgcgga cgagccagta ctggctcaga cgctgctgat gcagctaaag
     5041 caggtactgt caatgagcga tgcaaccgtt gctgagcata tgcaggattt gtatgccacg
     5101 ctgctgggcg acctgcaact actgaaagcc cgtcgcgggc tgagcgccag tgacctgatt
     5161 aatcttaatg ccgaccgcct gcaatgcctg ctgagcggtc atcctaaatt cgtttttaat
     5221 aaaggtcgcc gtggctgggg taaagaggcg ctggaacgat atgcgccaga gtatgccaac
     5281 accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg ctgtgataac
     5341 gagatggata ttcatcagtt gttgacggcc gcaatggatc cgcaggagtt tgcccgcttc
     5401 agtcaggtct ggcaggaaaa cggactggat cataactggc tgccgctgcc ggtacatccg
     5461 tggcagtggc agcaaaaaat cgctaccgac ttcatcgctg attttgccga aggcaggatg
     5521 gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg taccctgacc
     5581 aacgccagtc gccggggcgg gctggatatc aagctgccgc tgaccatcta caacacctca
     5641 tgctaccggg ggatccctgg cagatacatc gctgccggac cactggcttc acgctggcta
     5701 caacaggttt ttgcgaccga cgccacccta gtgcaaagcg gcgcagtgat acttggtgaa
     5761 ccggctgcag gctatgtctc ccatgaaggc tatgccgcgc ttgcccgggc tccctatcgc
     5821 taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct gaaaccggat
     5881 gaaagtccgg ttctgatggc aacactgatg gagtgcgacg aaaacaatca gccgctggca
     5941 ggcgcatata tagaccgctc cgggctggac gctgaaacct ggcttacgca actgttccgg
     6001 gtggtcgtgg ttcctctgta tcacctgctt tgccgctacg gtgtcgcgct tattgcacat
     6061 ggacaaaata taactctcgc catgaaagag ggggttccac agcgtgttct gctgaaagac
     6121 ttccagggcg atatgcgact ggtgaaagaa gagttccccg aaatggactc tttgcctcag
     6181 gaggttcgtg atgttacatc ccgcctgagt gcggactact taatccatga tttgcagacg
     6241 ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct tggcgtacct
     6301 gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa aaaacatcca
     6361 caaatgtcag agcgttttgc gcttttctca cttttcaggc cacaaatcat tcgcgtggtg
     6421 ctgaacccgg taaaactgac ctggcctgat ctggatggcg gcagccgcat gctgccgaat
     6481 taccttgagg atctgcaaaa tccgctgtgg ctggtaactc aggaatatga atcatgaaaa
     6541 aaagtgtcga ttttattggt gtagggacag ggccatttaa tctcagcatt gctgcgttgt
     6601 cacatcagat cgaagaactg gactgtctct tctttgatga acatcctcat ttttcctggc
     6661 atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa gatctggtca
     6721 gtgctgttgc acctacaaat ccctacagtt ttgttaacta tctggtgaag cacaaaaagt
     6781 tctatcgctt ccttacaagc agactacgta cagtatcccg tgaagagttt tctgactacc
     6841 tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt gaaaacattg
     6901 atttcgataa aaaacgtcga ttgtttctgg tgcaaaccag ccagggagaa tattttgccc
     6961 gcaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg aagcatatga
     7021 cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctt agtggaaaac
     7081 ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat gcattacgcg
     7141 gggaatgggg agaagcggcg gaaataaact gggtctcccg gcgtaataat tttaacgcac
     7201 tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca ggcttctccg
     7261 gactggagga agatattcgc catcagttac tggatgagca gaaaatgaca tcggatggca
     7321 tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt gaagttctga
     7381 gaaaaccaag aaatatccgt ctgctaccca gccgctcggt aacaactctg gaaagtagtg
     7441 gtcctggctg gaagttgctg atggagcatc atctggatca gggcagggag agcctggaaa
     7501 gtgatgtggt gattttcgcc acaggttacc gttctgcgtt gccacaaata cttccctcac
     7561 tgatgcccct gatcaccatg cacgataaga acacctttaa agtgcgtgat gacttcactc
     7621 tggaatggag tggcccgaaa gagaacaaca tcttcgtggt caacgccagt atgcaaaccc
     7681 atggcatcgc cgaaccccag ctcagcctga tggcatggag atctgcacgt attcttaatc
     7741 gcgtaatggg acgtgattta ttcgatctca gtatgccgcc cgccctgatt cagtggcgca
     7801 gcggcaccta gggaaaacgc agccggaggc tgcttcttta actcgctaca cagcatcttt
     7861 gggctgattt tttccgcccg tatggaggaa taatgatgat aagcaaaaag tatacgcttt
     7921 gggctctcaa cccactgctt cttaccatga tggcgccagc agtcgctcaa caaaccgatg
     7981 atgaaacgtt cgtggtgtct gccaaccgca gcaatcgcac cgtagcggag atggcgcaaa
     8041 ccacctgggt tatcgaaaac gccgaactgg aacagcagat tcagggcggc aaagagctta
     8101 aagacgcact ggctcagctg atccctggcc ttgacgtcag cagccggagc cgcaccaact
     8161 acggtatgaa tgtgcgtggc cgcccgctgg tcgtgctggt tgacggcgtg cgtctcaact
     8221 cttcacgtac cgacagccga caactggact ctatagatcc ttttaatatc gaccatattg
     8281 aagtgatctc cggtgcgacg tccctgtacg gcggcggcag taccggtggc ctgatcaaca
     8341 tcgtgaccaa aaaaggccag ccggaaacca tgatggagtt tgaggctggc accaaaagtg
     8401 gctttagcag cagtaaagat cacgatgaac gcattgccgg agctgtctcc ggcggaaatg
     8461 agcatatctc cggacgtctt tccgtggcat atcagaaatt tggcggctgg tttgacggta
     8521 acggcgatgc caccttgctt gataacaccc agaccggcct gcagtactcc gatcggctgg
     8581 acatcatggg aactggtacg ctgaacatcg atgaatcccg gcagcttcag ttgatcacac
     8641 agtactataa aagccagggc gacgacgatt acgggcttaa tctcgggaaa ggcttctctg
     8701 ccatcagagg gaccagcacg ccattcgtca gtaacgggct gaattccgac cgtattcccg
     8761 gcactgagcg gcatttgatc agcctgcagt actctgacag cgcttttctg ggacaggagc
     8821 tggtcggtca ggtttactac cgcgatgagt cgttgcgatt ctacccgttc ccgacggtaa
     8881 atgcgaacaa acaggtgacg gctttctctt cgtcacagca ggacaccgac cagtacggca
     8941 tgaaactgac tctgaacagc aaaccgatgg acggctggca aatcacctgg gggctggatg
     9001 ctgatcatga gcgctttacc tccaaccaga tgttcttcga cctggctcag gcaagcgctt
     9061 ccggagggct gaacaacaag aagatttaca ccaccgggcg ctatccgtcg tatgacatca
     9121 ccaacctggc ggccttcctg caatcaggct atgacatcaa taatctcttt accctcaacg
     9181 gtggcgtacg ctatcagtac actgaaaaca agattgatga tttcatcggc tacgcgcagc
     9241 aacggcagat tgccgccggg aaggctacat ccgccgacgc cattcctggc ggctcagtcg
     9301 attacgacaa cttcctgttc aacgccggtc tgctgatgca catcaccgaa cgccagcagg
     9361 catggctcaa cttctcccag ggcgtggagc tgccggaccc gggtaaatac tatggtcgcg
     9421 gcatctatgg tgctgcagtg aacggccatc ttcctctaac aaagagtgtg aacgtcagcg
     9481 acagcaagct ggaaggcgtg aaagtcgatt cttatgagct gggctggcgc tttactggca
     9541 ataatctgcg tacccaaatc gcggcctact attcgatttc tgataagagc gtggtggcga
     9601 ataaagatct gaccatcagc gtggtggacg acaaacgccg tatttacggc gtggaaggtg
     9661 cggtggacta cctgattcct gatactgact ggagtaccgg agtgaacttc aacgtgctga
     9721 aaactgagtc gaaagtgaac ggtacctggc agaaatacga tgtgaagaca gcaagcccat
     9781 caaaagcgac agcctacatt ggctgggcac cggacccgtg gagtctgcgc gtgcagagca
     9841 ccacctcctt tgacgtgagc gacgcgcagg gctacaaggt cgatggctat accaccgtgg
     9901 atctgctcgg cagttatcag cttccggtgg gtacactcag cttcagcatt gaaaaccttt
     9961 tcgaccgtga ctacaccact gtctgggggc agcgtgcacc actgtactac agcccgggtt
    10021 acggcccagc gtcactgtac gactacaaag gcaggggccg aacctttggt ctgaactact
    10081 ctgtgctgtt ctgaccggta ttcctttaca acaaaggtac gctgatatca acatggccgc
    10141 tgacagccaa gttgatatca tataatacac gacataatct gtagtcaggg aggatagact
    10201 ctttactgac tacagattat gtcgtgttcc gtgctcattt cctcaaaaaa atacaagaaa
    10261 agaattagta ttctaacaaa aagtgaaata aattgtatca aactccctct tttaatcctg
    10321 ttgagtaaat cagcttttgc aataggattg aaagagtgta agtggaatct cttccggata
    10381 ctcgttacca ccgtggctag aatatctacg gctgcg
//
LOCUS       DAJOWB010000145        10200 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJOWB010000145 DAJOWB010000000
VERSION     DAJOWB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.145"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            74..5494
                     /locus_tag="OHO85_004096"
     CDS             74..5494
                     /locus_tag="OHO85_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
                     /protein_id="HCQ2673397.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGMGEATIREGGLVTAENTIIGGNATGV
                     GTLNVQDQDSVITVRRLYNGYFGNGAVNISNNGLINNKEYSLVGVEDGSHGVVNVTDK
                     GHWSFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGENGVGNVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDGTSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGTGEL
                     NITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLN
                     VEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGYITNATKVEFGSGEGVFVFNHTNNSD
                     AGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMG
                     SSEVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSSSDKMFGFTHATGTEFA
                     GVAQLKDSTFTLERDNTAALTHAMLQSDSENTTSVKVGEQSIGGLAMNGGTLIFDTDI
                     PAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLN
                     LLEHDDSHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGF
                     RLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVR
                     QVSLSNGQNDYQGATYVQMGTLRTDADGALGNTRELNISNATIVDLNGSTQTVETFTG
                     QMGSTVLFKEGALTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSISPNAE
                     VSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVG
                     QFNIDTGSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDVTKLGTGILT
                     LNNDSAAYQGTTDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVNTAGDVNVMPGG
                     TLRVAKTTIGGNLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPT
                     DKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVY
                     TLAKGKGNDEKNWYLTSKWDGVTPPDTPNPINNPPVVDPEGPSVYRPEAGSYISNIAA
                     ANSLFSHRLHDRLGEPQYTDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVL
                     QLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGIYATWYQ
                     NDANKTGAYVDSWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEG
                     TLNTWYVQPQVQITWMGVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDG
                     KQREFQPYIEANWINNSKVYAVKMNGQTVSRDGARNLGEVRTGVEAKVNNNLSLWGNV
                     GVQLGDKGYSDTQGMLGVKYSW"
     gene            5703..5969
                     /gene="hipB"
                     /locus_tag="OHO85_004097"
     CDS             5703..5969
                     /gene="hipB"
                     /locus_tag="OHO85_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HCQ2673398.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            5969..7291
                     /gene="hipA"
                     /locus_tag="OHO85_004098"
     CDS             5969..7291
                     /gene="hipA"
                     /locus_tag="OHO85_004098"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HCQ2673399.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            complement(7489..8430)
                     /locus_tag="OHO85_004099"
     CDS             complement(7489..8430)
                     /locus_tag="OHO85_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673400.1"
                     /translation="MAIINIYSKRQRKIRGEVNDVYQYNNIPHALRVQIIKIITDSIG
                     LPSSNERYTSYRNEADKVYAYIHEILSKEYGVFSLKEFAKNDFDALVDFFLKERNTEK
                     CLDFIEICFQILVSHVAKNHYEFKDITSQSPGDAVIELNERFREHGVGYQFESEEIIR
                     VDSQLIHADVVKPTLILLSGEPLFEGANDEFLAAHEHYRHKRYKECLNDCLKSFESIM
                     KAIHDKNNWKYSPNDTASKLINSCLSQNLIPAYLQSQFTSLKTMLETGIPTIRNKNAG
                     HGQGADIKEVPEELASYMLHLTATNLLFLLKCEKNIK"
     gene            complement(8665..10065)
                     /locus_tag="OHO85_004100"
     CDS             complement(8665..10065)
                     /locus_tag="OHO85_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCQ2673401.1"
                     /translation="MAINDAGMFTVKEINRLKILQDVIDRNIRPGQAAEMLGITPRHC
                     SRLLKRYRQYGPLGMNNQSRGRTGNRLLPASLTDQALSIIREHYRDFGPTLAREKLEE
                     VHGLVLGKETIRRLMIKAGLWIPRRQRAPKIHQPRYRRPCTGELIQIDGCDHHWFENR
                     GRPCTALVYVDDATSRLMHLLFVKSESTFTYFEATRGYIEKYGKPMILYSDKASVFRV
                     NNKHATTGPGETQFARAMRCLNITPLCAETSQAKGRVERAHLTLQDRLVKELRLKGIC
                     TIEAANAFAEEYMADYNRRFAKEPLHDFNAHRPLALDDDLDAEFTWREPRRVSKNLTV
                     QYDKMLYLIEDNEYSRRAIGKYIDVWHYPDGHKELRLNGVLLPYSTYDRLSEVDPVAI
                     VDNKRLGHVLDVARQVQRKRDNNRSQSLPCSGDEPSRRRHAPSINKSQRSLNEDDLLE
                     AMIKLQGSSEAIFGKR"
BASE COUNT         2720 a         2202 c         2687 g         2591 t
ORIGIN      
        1 taataagttt catttttaat gtctgacgac tcatctcttt cgtggctgta ttgtttgtat
       61 atggagcgtt tatatgaata gaatctatcg cgtgatatgg aattgcactc tacaggtatt
      121 tcaggcctgc tcggaattaa ctcgcagggc aggtaaaaca tcgacggtta atttgcgtaa
      181 atcctctgga ctgacaacga aattcagtag attgacgctg ggtgttttgc tggcactaag
      241 cggttcagcg tctggtgcaa gtctggaagt tgataatgat cagattacca atattgatac
      301 tgatgttgct tatgatgcct acctggttgg ctggtatggc actggagtgc ttaatatttt
      361 ggctggcggt aatgcctcct taaccactat tactaccagc gtcattggcg ctaatgagga
      421 ctcagagggc accgttaatg ttttgggtgg cacctggcga ttgtatgata gcggaaataa
      481 tgcaaggcct ttaaatgtgg gtcaatccgg aacggggacg ctgaatatta aacagaaggg
      541 tcacgtcgat ggaggctatt taagattagg ttcttcgaca ggaggcgtcg ggacggtcaa
      601 tgttgaggga gaggactctg ttctgacgac cgaattattc gaaataggga gttacggtac
      661 gggctcatta aatattacgg ataagggtta cgtcacgagt tcaatcgtcg ccattttagg
      721 ctatcaggcg ggcagtaatg gccaggttgt cgttgaaaag ggtggcgagt ggctaataaa
      781 aaataacgat tcctcaattg aatttcaaat tggtaatcaa gggatggggg aagcgactat
      841 tcgcgagggg gggctggtta cggctgaaaa tacgattatc ggtggcaatg ccaccggtgt
      901 cggaaccctg aatgtgcagg atcaagactc tgtcatcacg gtacgcagac tctataatgg
      961 atatttcggt aatggcgcag tcaatatttc caataatgga ctaattaata acaaagaata
     1021 ttcattggtg ggcgttgaag acggttccca cggtgtcgtc aacgtgaccg ataaagggca
     1081 ttggagtttc ctcggaacag gcgaagcttt ccgctatatc tatatcggtg atgctggtga
     1141 cggtgaactt aatgtctcgc gtgaaggtaa agtagattcg ggaattatca ctgcggggat
     1201 gaaagaaaca ggcacaggca acattactgt taaggataag aactccgtta tcactaatct
     1261 cggaactaat cttggttatg acggccacgg cgaaatgaat atcagtaatg aggggcttgt
     1321 tgtcagcaac ggaggaagct cactcggtta tggagaaaac ggcgtcggga atgtcagcat
     1381 caccacgggg gggatgtggg aggtcaataa gaatgtctat accaccattg gtgttgcggg
     1441 cgtcggaaac ctcaatatta gcgatggcgg taagtttgta tcgcaaaata ttactttttt
     1501 gggcgataaa gcaagcggta tcggcacact gaacctgatg gatgggacat catcgttcga
     1561 tactgtgggt atcaatgtcg gtaattttgg tagcggtatc gtaaatgtca gtaatggtgc
     1621 cacccttaat tcaacgggct atggatttat cggaggaaat gcctccggta aagggatagt
     1681 taatatttca acggatagtc tctggaattt aaagacatca tctactaacg cccaattgct
     1741 acaggtcggt gtattaggca cgggtgaact gaatattacc accggaggta tagttaaagc
     1801 gcgtgataca cagatagctc tcaatgacaa aagtaagggc gacgtgaggg tggatgggca
     1861 gaactctctt cttgaaacat tcaatatgta cgtagggaca tctggtacgg gtacgttaac
     1921 cctgacgaat agcggcacgc tgaatgtcga aggtggagaa gtttacttag gtgtttttga
     1981 acctgctgta ggaacgctaa acattggtgc agctcacggt gaggcggcgg cagatgccgg
     2041 gtatataacc aacgcgacga aagtagagtt tggttctggc gaaggtgttt ttgtctttaa
     2101 tcatactaat aacagtgatg ccggctacca ggtcgatatg ctgattacgg gtgacgataa
     2161 agacggaaaa gtgatccatg atgcaggcca tacggtgttc aatgcaggga atacttatag
     2221 cggtaaaacg ctggtcaatg acggcctcct gaccattgcg tctcatacgg cagatggggt
     2281 aacgggcatg ggttcgagtg aagtcaccat tgcaagcccc ggtacgctcg acattctcgc
     2341 atcaacgaac agtgcaggag attacacgct gaccaatgcg ctcaaaggcg atggtttgat
     2401 gcgagtgcag ctgtcatcct ccgacaagat gtttggcttt acccatgcaa cagggactga
     2461 attcgccggt gttgcccaac tgaaagacag taccttcact ctggaacgcg acaacaccgc
     2521 tgcgcttact cacgcgatgt tgcagtctga cagtgaaaat accacatcgg taaaagtggg
     2581 agagcaatcc attggtggac tggccatgaa tggcggtacg ctcattttcg atacggatat
     2641 tcctgctgcg acgcttgcag agggatatat cagcgtcgat acgctggttg tcggcgcggg
     2701 tgactacacc tggaaaggcc gtaactatca ggtaaacggg acgggcgacg tgcttatcga
     2761 cgtgcctaaa ccgtggaatg atcccatggc gaataaccct ctgacgacgc tcaatttgct
     2821 ggaacacgac gatagccatg tcggcgttca actggtgaag gcgcaaacgg ttattgggtc
     2881 gggtggctca ttaacgttac gtgatttaca gggcgacgag gtggaagcgg acaaaacgtt
     2941 acacattgcg caaaacggaa cggtggtcgc cgagggtgat tatggattcc gcctcacgac
     3001 cgcaccaggt gatggtttgt acgttaacta tgggctgaaa gcgctgaaca tccatggtgg
     3061 gcaaaagctg acgttagccg aacatggcgg agcctatggc gcaacggccg atatgtcggc
     3121 aaaaatcggt ggtgaagggg atctggcaat caatacggtg cgacaggttt cgctttccaa
     3181 cggtcagaac gactatcagg gggcaaccta cgttcagatg gggacattac gtaccgatgc
     3241 ggatggtgca cttggcaaca cccgggaact gaacatcagc aacgcgacca tcgtcgatct
     3301 taatggatcg acgcagacgg tagagacatt caccgggcag atgggttcga ctgttttgtt
     3361 caaagagggg gcgctgacgg taaataaagg tgggatcagt cagggtgaac tgacaggtgg
     3421 cggaaacctg aatgttacag ggggaacgct ggctatcgag gggcttaatg cacgctacaa
     3481 tgcgttaacc agcattagcc caaatgcgga agtcagcctc gataatactc aggggttagg
     3541 cagaggaaat attgccaatg acggtctgtt aacgctaaaa aacgtgactg gcgaactgcg
     3601 taatagcata agcgggaagg gtatcgtgag cgcaaccgcc aggacagatg tagagctgga
     3661 tggcgataat agccgctttg tggggcaatt caacattgat acaggcagcg cgctcagcgt
     3721 caacgagcag aaaaacctgg gtgatgcttc cgttatcaat aatggcctgc tcaccatctc
     3781 cactgagcgt agctgggcga tgacgcacag tatcagcggt agcggtgatg tgacaaaact
     3841 gggtaccggg atcctgactc ttaacaacga ttccgcggcg tatcagggta cgacggatat
     3901 cgtggggggg gaaattgctt tcggttccga ctctgccatt aatatggcaa gtcaacacat
     3961 taatatccat aacagcggtg tgatgtcggg aaatgtcaac actgcaggtg atgtgaacgt
     4021 tatgcctggg gggacactgc gtgtcgctaa aaccacaatc ggcggcaacc tggagaatgg
     4081 cggcacggtt caaatgaata gcgaaggggg gaaaccgggg aatgtactga ccgttaacgg
     4141 caactatacc ggaaacaatg gcctgatgac gttcaacgcg acgctgggcg gcgataattc
     4201 gcccaccgat aagatgaacg tgaaaggcga tacccaaggg aacactcgcg ttcgggttga
     4261 taacattggc ggcgtcggtg cacaaacggt caacggtatt gaactcattg aggttggcgg
     4321 taattctgca ggtaacttcg cgctgacaac cggaactgtc gaagctgggg cttacgtcta
     4381 cacgctggct aaagggaagg ggaatgacga gaaaaactgg tatctgacca gtaaatggga
     4441 cggcgtaacg ccaccggata cacccaaccc catcaataat ccccctgttg tggatccgga
     4501 aggcccatca gtttatcgcc cggaggccgg aagctatatc agcaacattg ccgcagccaa
     4561 ctcgctgttt agccatcgtt tacacgaccg tttgggtgag ccgcagtata cagattcact
     4621 gcattctcag gggtcggcaa gcagtatgtg gatgcgtcat gtcggagggc acgaacgttc
     4681 aagggccggt gacggtcagc taaatactca ggctaaccgc tatgtattgc agctaggcgg
     4741 cgatttggcg cagtggagta gcaacgcgca ggatcgctgg catcttggcg tgatggcagg
     4801 ctacgccaat cagcacagta atactcagag taatcgtgtg ggttataaat cggatgggcg
     4861 catcagcggg tacagcgctg ggatttacgc gacctggtat cagaacgatg cgaataagac
     4921 cggcgcttat gttgacagct gggcgctgta taactggttt gataacagcg tcagttccga
     4981 taaccgttct gctgacgact atgattctcg cggtgtgacg gcctctgttg agggtgggta
     5041 tacctttgaa gcgggaacat ttagcggcag cgaagggacg ctgaatacct ggtacgtcca
     5101 gccacaggtg caaatcacct ggatgggtgt gaaagattct gaccataccc ggaaagacgg
     5161 aacacgcatt gaaacggaag gcgacggaaa cgtgcaaacg cgacttgggg tgaaaaccta
     5221 cctgaacagc catcaccagc gtgacgatgg taaacagcgt gagttccagc cttacattga
     5281 agcgaactgg atcaacaata gcaaagtcta cgccgtgaag atgaatggtc aaaccgtaag
     5341 ccgtgatggt gcgcgtaatc tcggtgaagt acgtaccggg gttgaggcga aagtaaataa
     5401 caaccttagc ctgtggggga atgtcggtgt gcaactaggt gataaaggct atagcgatac
     5461 tcagggcatg ctgggagtga aatatagctg gtaaaccgta taagccgcat gtcgagatgg
     5521 catgcggctt aatattgccg acttcaaagg ttttaaagcc tcccttttat ccgtgatcgc
     5581 ggatatcgca gcgtttatcc cgtacagcgg ataagatgtg tttccagatt gactttatcc
     5641 tcactaaagg ataaaactga taatatcccc ttaagcggat aaatttgctg tggacgtatg
     5701 acatgatgag ctttcagaag atctatagcc caacgcaatt agcgaatgca atgaaactgg
     5761 ttcgccagca aaatggctgg acgcagagcg agctggcgaa aaaaattggc attaagcagg
     5821 cgacaatttc caatttcgaa aacaaccctg acaataccac gctcacgaca ttttttaaga
     5881 ttttacagtc gcttgaactc tcaatgacac tatgcgacac gaaaaatgcc tcgccagaat
     5941 caacagaaca gcaagatctg gagtggtaat gcctaaactt gtcacttgga tgaacaacca
     6001 gcgggtaggc gagttaacga agttagccaa cggcgcgcac acctttaagt atgcaccgga
     6061 gtggttagcc agccgttatg ccagaccgtt gtcactttcg ctgccattgc agagggggaa
     6121 tatcacctca gatgccgtat ttaacttctt cgataacctg ttacccgata gcccgattgt
     6181 acgtgaccgg atcgttaaac gttatcatgc taaatccaga caaccgtttg atttattgtc
     6241 agaaataggg cgagacagcg ttggtgccgt aacgttatta cccgaaaatg aaaccataac
     6301 gcgcccgata atggcatggg aaaagcttac tgaagccagg cttgaagaag tattaacggc
     6361 ttataaagca gatatccctc tgggcatgat tagagaagaa aatgactttc gcatctcggt
     6421 tgctggcgcg caggagaaga cagcgctgct tagaataggc aatgactggt gcattccgaa
     6481 aggaataacg ccgacgacgc acatcattaa attaccgatt ggcgaaatca ggcagcccaa
     6541 tgcgacgctc gatctcagcc aaagcgttga taatgagtat tactgtctgc tgctggcgaa
     6601 agaacttggg ttgaatgttc cggacgcaga aatcattaaa gcgggaaggg tgcgcgcgtt
     6661 agcggtcgaa cgttttgaca ggcgttggaa tactgagcga acggttttac ttcgcttgcc
     6721 acaggaggat atgtgtcaga cattcggttt accttcatct gtgaaatatg aatcagatgg
     6781 aggcccaggc atcgcgcgga tcatggcgtt tttgatgggg tccagcgagg cgcttcgcga
     6841 tcgttatgat tttatgaaat tccaggtctt ccagtggttg attggcgcaa cggatggcca
     6901 tgcaaaaaac ttctccgtat ttattcaggc tggcggcagt tatcgactca cgccatttta
     6961 cgacatcatt tcagcatttc cggtccttgg cggtacggga atacacatca gcgatctcaa
     7021 actggcaatg gggcttaacg catccaaagg caaaaaaacg gcaatcgata aaatttatcc
     7081 gcgacatttt ttggcgacag caaaggtgct gagattcccg gaagtgcaga tgcatgaaat
     7141 cctaagtgac tttgccagaa tgattccggc agcactggat aacgtgaaga cttcattacc
     7201 gacagatttt ccggagaacg tggtgacggc agttgaaacc aatgtgttga ggttgcatgg
     7261 acggttaagc cgagaatacg gtagtaagtg agatatgggg tttggtcgtt gttaatgagc
     7321 acgacaatca tgaccgccaa cgactggatt cttccttggt ttatacttgt caaagctcag
     7381 tgaaaatgtc taatcactgg ctcattataa atgtccatat gttgaaaagg ccgctgcgaa
     7441 aatccctccc gcagcggcct ctttagcata acgtcattat gcgaaccatc acttaatgtt
     7501 cttctcgcat tttaagagaa atagtaaatt tgtagcagta agatgaagca tatagcttgc
     7561 caattcttcc ggaacctctt taatatcagc tccttgccca tgacctgcat ttttgtttct
     7621 gatggttgga attcctgttt ccagcattgt tttcagactt gtgaactggc tttgtaaata
     7681 tgccggaatt aaattttgac ttaaacagct atttattagt tttgatgctg tatcattagg
     7741 actatatttc cagttattct tatcatgtat tgctttcatt atactttcaa atgatttcag
     7801 acaatcattt agacattctt tataacgctt atgcctgtaa tgctcatgcg cagcaaggaa
     7861 ttcatcattg gcgccttcaa acaagggttc tccacttaaa agaatcaggg ttggctttac
     7921 gacatcagca tgaatcaatt gagagtctac ccttattatc tcttcagatt caaactgata
     7981 accaacccca tgctctctaa acctttcatt tagttcaata actgcatccc caggactttg
     8041 acttgttatg tctttgaatt cataatggtt tttcgcaacg tggctcacta gtatctggaa
     8101 gcatatttct atgaaatcta ggcatttctc tgtatttctc tctttaagga aaaagtcgac
     8161 aagagcgtca aagtcattct ttgcaaattc cttcaaagaa aaaacaccat attccttaga
     8221 aagtatctca tggatatatg catatacttt gtcagcttca tttctatacg atgtgtagcg
     8281 ttcattagaa gagggtaagc cgatagaatc agttattatt ttaattattt gcaccctgag
     8341 tgcatgtgga atattgttat attgatatac gtcatttact tcgccacgaa tctttctttg
     8401 tctcttagaa taaatattaa taattgccat gaaatagcct caagattttt ttacatgctg
     8461 ttctgtgata cttgctgttt ggctctttgc accgcgaagc ggttcggggt tctgtgacac
     8521 ccgaatttta cctcggtttc ctttgtatat aatatcattg aagttatttg catttctcag
     8581 aaatcttctc tggctttaat tgcaatagcc gaaagatagc gcacacatcg ccgttggtca
     8641 gttagtacga ggtttccgcc aagttcacct tttaccaaag atggcctcag agcttccctg
     8701 aagctttatc atggcttcaa gtaggtcatc ttcgttcagt gagcgctgag atttgttgat
     8761 gctaggggcg tgccgtctgc gggaaggctc atcaccagaa caaggtaacg attgtgagcg
     8821 attattgtcc cgttttctct ggacctgacg ggcaacatcc aacacatggc caagtctctt
     8881 gttatctaca atggcgacgg gatcaacctc agaaagccgg tcataggtgg agtagggcag
     8941 cagcacgcca tttagtcgca gctccttatg tccgtcagga taatgccaga catcaatata
     9001 cttgccgatg gctcttcgac tgtactcgtt gtcctcaatc agatagagca ttttatcgta
     9061 ttgtacagtc agattttttg atacccggcg aggctctcgc caggtaaatt ctgcatcaag
     9121 gtcatcgtct aatgccagcg gccggtgcgc gttaaaatca tgcaagggtt ctttggcaaa
     9181 gcgccggtta taatccgcca tgtattcttc tgcaaatgca ttggccgctt ctattgtaca
     9241 gatcccctta agcctgagct ccttaacgag tcggtcctga agtgtcaaat gagcacgttc
     9301 aacgcggcct tttgcctggc tggtttcagc acacaaagga gtaatattaa ggcagcgcat
     9361 ggccctggca aactgggttt cgccggggcc ggtggtggcg tgcttattat tgactctgaa
     9421 tacgctggct ttatcgctgt agaggatcat cggtttaccg tatttctcga tatagccacg
     9481 tgtagcttca aaataggtaa atgtggactc agatttcaca aacagcaggt gcatcaggcg
     9541 actggtggca tcgtcaacat agaccagggc agtacagggt cgaccccggt tttcaaacca
     9601 gtggtggtcg cagccatcaa tttgtatcag ctcgccagta caaggacgcc ggtaacgagg
     9661 ctgatgaatt ttcggtgcgc gttgtctgcg gggaatccag agtccggcct ttatcatgag
     9721 acgtcggatg gtttcctttc caaggaccag accgtgaact tcctccagct tttcccgcgc
     9781 tagcgtgggg ccaaaatcac ggtagtgctc ccggattata ctgagtgcct gatctgtcag
     9841 tgacgctggc aacagacggt taccagtgcg gccacgactc tggttgttca tgccaagcgg
     9901 cccatactgg cgataacgtt tgagcagacg gctgcaatga cgtggcgtga tgcccagcat
     9961 ttcggcagcc tgtccaggac ggatattgcg atcgatgaca tcctgaagga ttttgaggcg
    10021 attgatttct ttcacggtga acattccggc gtcatttata gccatagaaa actcctcatc
    10081 aggaaaaagt gagtcgttgt ctagacatag ggatctgaga aactatagcc tggacattcc
    10141 taatgagcca caaatagaca ttagcattga gcctctacaa tactggtgcg cataatgtat
//
LOCUS       DAJOWB010000146        10134 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJOWB010000146 DAJOWB010000000
VERSION     DAJOWB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.146"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..561
                     /gene="ssuE"
                     /locus_tag="OHO85_004101"
     CDS             1..561
                     /gene="ssuE"
                     /locus_tag="OHO85_004101"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCQ2673402.1"
                     /translation="MAGSPRFPSRSSSLLEYAREKLNCLDVEVYHWNLQNFAPEDLLY
                     ARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVVLPLAT
                     GGTVAHLLAVDYALKPVLSAMKAQEILHGVFADDSQVIDYHHKPQFTPNLQTRLDTAL
                     ETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            554..1513
                     /gene="ssuA"
                     /locus_tag="OHO85_004102"
     CDS             554..1513
                     /gene="ssuA"
                     /locus_tag="OHO85_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCQ2673403.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VTAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1510..2655
                     /gene="ssuD"
                     /locus_tag="OHO85_004103"
     CDS             1510..2655
                     /gene="ssuD"
                     /locus_tag="OHO85_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCQ2673404.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2666..3457
                     /gene="ssuC"
                     /locus_tag="OHO85_004104"
     CDS             2666..3457
                     /gene="ssuC"
                     /locus_tag="OHO85_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HCQ2673405.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3454..4221
                     /gene="ssuB"
                     /locus_tag="OHO85_004105"
     CDS             3454..4221
                     /gene="ssuB"
                     /locus_tag="OHO85_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCQ2673406.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4319..6931)
                     /gene="pepN"
                     /locus_tag="OHO85_004106"
     CDS             complement(4319..6931)
                     /gene="pepN"
                     /locus_tag="OHO85_004106"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCQ2673407.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQCTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7197..8399
                     /gene="pncB"
                     /locus_tag="OHO85_004107"
     CDS             7197..8399
                     /gene="pncB"
                     /locus_tag="OHO85_004107"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCQ2673408.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYKKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8568..9968
                     /gene="asnS"
                     /locus_tag="OHO85_004108"
     CDS             8568..9968
                     /gene="asnS"
                     /locus_tag="OHO85_004108"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCQ2673409.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2263 a         2638 c         2744 g         2489 t
ORIGIN      
        1 ctggcgggta gtcctcgctt tccttctcgc tccagctcct tgctggaata tgcgcgggaa
       61 aaactaaatt gcctggatgt agaggtttat cactggaatc tgcaaaactt cgccccggaa
      121 gatctgcttt acgctcgttt cgatagtccg gcactcaaga ccttcaccga acagctgcaa
      181 caggctgatg gactgatagt cgctacacct gtgtataaag ccgcctattc cggcgcgctg
      241 aaaaccctgc tcgacctgct gccagagcgc gctttgcaag gcaaagtggt gctaccgctg
      301 gcgacgggcg gtacggtggc tcatctgctg gcggtcgatt atgcccttaa gccagtttta
      361 agtgcgatga aagcccagga gatcctgcac ggcgtgtttg ccgatgattc acaagttatt
      421 gattaccatc acaaacccca gttcacgcca aatctgcaaa cccgtcttga taccgcgcta
      481 gaaactttct ggcaggcatt gcaccgccgc gatgttcagg ttcctgacct tctgtctctg
      541 cgaggtaatg tccatgcgta acatcattaa actggcgctg gcgggattgc ttagcgtctc
      601 tacttttgcg gttgctgcag aatcctcgcc tgaagcgtta cgtataggct atcagaaagg
      661 cagtattggt atggtattgg caaaaagcca ccagttactg gaaaaacgct atccgcaatc
      721 aaaaatctcc tgggtggagt tccccgcggg tccgcaaatg ttggaagcgt taaacgttgg
      781 cagtattgat ctcggcagta ccggggatat tccgccaatc tttgcccagg ctgccggggc
      841 tgatttggta tatgtgggcg tcgagccacc gaagcccaaa gccgaagtga ttctggtggc
      901 agaaaacagc ccgatcaaaa ccgtagccga tcttaaaggt cacaaagttg cctttcagaa
      961 aggttccagt tcacacaacc ttttactgcg tgcactgcgt caggccggac ttaagtttac
     1021 cgatatccaa cccacttacc tgacgcccgc tgatgcccgc gccgcgttcc agcaaggtaa
     1081 cgttgacgcc tgggctatct gggatcccta ctactctgct gcattattac agggcggcgt
     1141 gcgggtgctg aaagacggca ccgatctcaa tcaaactgga tcgttttatc tggcagctcg
     1201 tccctatgca gaaaaaaacg gcgcttttat tcagggcgta ctcgcaacct ttagtgaggc
     1261 cgatgcgtta acccgcagcc agcgcgagca aagcatcgct ttactggcaa aaacgatggg
     1321 cttaccggca ccggtgattg cctcttactt agatcatcgc cctcctacca ccatcaaacc
     1381 ggttaccgcc gaggttgccg ccttacagca gcaaacggca gatctgtttt atgaaaatcg
     1441 tctggtgccg aaaaaagtcg atattcgcca gcgcatctgg cagcccactc aactggaagg
     1501 aaaacaatta tgagtctgaa tatgttctgg tttttaccga cccatggtga cgggcattat
     1561 cttggaacgg aagaaggttc acgcccggtt gatcacggct atctgcaaca aattgcgcaa
     1621 gcggcggatc gtctgggcta taccggtgtg ctgatcccaa cggggcgatc gtgtgaagat
     1681 gcgtggctgg tggcggcgtc gatgatcccg gtgacgcagc ggctgaagtt tcttgtcgcc
     1741 ctgcgcccca gcgtaacctc acctaccgtt gccgctcgcc aggccgccac gcttgaccgt
     1801 ctctcaaatg gtcgtgcgtt gtttaatctg gtcacaggca gcgatccaca agagctggca
     1861 ggcgacggcg tgttccttga tcatagcgag cgctacgaag cctcggcgga atttacccag
     1921 gtctggcggc gcttattgct gggggaaacc gtcaatttca acggtaaaca tattcatgtg
     1981 cgcggagcaa aactgctctt cccgccgatt caacagcctt atcctccgct ttactttggc
     2041 ggatcgtcag acgtcgccca ggagctggcg gcagaacaag ttgatctcta cctcacctgg
     2101 ggcgaaccgc cagaactagt aaaagagaag atcgaacaag tgcgggcgaa agctgccgcg
     2161 catggacgca aaattcgttt cggtattcgt ctgcatgtga ttgttcgtga aactaacgac
     2221 gaagcgtggc aggccgccga gcggttaatc tcgcatcttg atgatgaaac tatcgccaaa
     2281 gcacaggccg cattcgcccg gacggattcc gtaggacaac agcgaatggc ggcgttacat
     2341 aacggcaagc gcgacaatct ggagatcagc cccaatttat gggcgggcgt tggcttagtg
     2401 cgcggcggtg ccggaacggc gctggtgggc gatggtccta cggtcgctgc gcgaatcaac
     2461 gaatacgccg cgcttggcat cgacagcttt gtgctttcgg gctatccgca tctggaagaa
     2521 gcgtatcggg tcggcgagtt gctgttccca catctggatg tcgccattcc ggaaattccc
     2581 cagccgcagc cgctgaatcc gcaaggcgaa gcggtggcga atgattttat cccccgtaaa
     2641 gtcgcgcaaa gctaaggaag aaataatggc aacgccagtg aagaagtggt tattgcgcgt
     2701 tgccccctgg tttttaccgg tgggcatcgt ggcggtgtgg caactggcct cctcagttgg
     2761 ctggctgtcg acgcgtattt tgccctcacc ggaaggggtg gtgacggcgt tctggacgct
     2821 ctccgccagc ggcgaactgt ggcagcatct ggcgatcagt tcctggcggg cgctgattgg
     2881 cttttcaatt ggcggatcgc tggggttgat tttgggactg attagcgggc tatctcgctg
     2941 gggagaacgg ctgctggata cctcaattca gatgttgcgc aacgtaccgc atctggcgct
     3001 gatcccgctg gtgattttgt ggtttggcat tgatgaatcc gcaaaaatct ttctggtggc
     3061 gctcggcacg ctattcccca tttatatcaa cacctggcat ggcatccgta atatcgatcg
     3121 cggtctggtg gaaatggcgc gtagctatgg attatccggc ataccgctgt ttatccatgt
     3181 gatcctgcct ggtgccctgc cctcaattat ggtcggcgtg cgttttgcgt tgggcctgat
     3241 gtggctgacg ctgattgttg ccgaaaccat ttctgccaat tcaggcattg gttatctggc
     3301 gatgaatgcg cgggagtttt tgcaaacgga cgtggtggta gtcgctatta ttctttacgc
     3361 cctgctcggc aaacttgctg acgtcagcgc gcagctgtta gagcgcctct ggttgcgctg
     3421 gaacccggct tatcatttga aggaggccac ggtatgaata ctgctcgtct gaaccagggc
     3481 acaccattgt tgctcaatgc agtaagcaaa cattacgcgg aaaatatcgt cctgaaccaa
     3541 ctggatttac atattccggc aggtcagttt gtggcggtgg tgggccgcag cggtggtggc
     3601 aaaagtaccc tgctgcgcct gctggcaggt ctggaaacgc caaccgcagg cgatgtgtta
     3661 gcgggcacca caccgctggc tgagattcag gaagacacgc gaatgatgtt tcaggatgcg
     3721 cgtctgctgc cgtggaaatc ggtgattgat aacgttgggt taggccttaa aggccagtgg
     3781 cgcgatgccg cgcgtcgggc gttggctgca gtaggactgg agaatcgcgc cggggaatgg
     3841 cctgccgcac tttctggcgg gcagaaacag cgtgtggcgc tggcaagagc gttgattcat
     3901 cgaccgggtt tattgttgct tgatgaaccg ctcggggcgc tggacgcctt aacgcgactc
     3961 gagatgcagg atttgattgt gtcactttgg cagcagcacg gctttaccgt actgttggtg
     4021 acgcatgatg tgagcgaagc ggtagcaatg gctgaccggg tgttgttaat tgaagaggga
     4081 aaaattggtc tggatttgac agtggatatc ccccgtccgc gtcgattagg gtcggtgcga
     4141 ttagcggagc tggaagcgga agtattgcaa cgggtaatgc ggcgtgggca ttctgaacaa
     4201 ctgattcggc gtcatggtta atatgccgga tgtggcgcac aaaaccagca ttttcaacgg
     4261 agttgtaggc ctgataagac gcgtcaagcg tcgcatcagg cattttggct caaaagtatc
     4321 aagccagtgc tttggtgatc ttctcgtaca gatcgccaga gagattttcc agccctttca
     4381 actgttccag cgccgcgcgc attttctcct gacgtttggc atcataacgt ttcaggcgaa
     4441 tcagcggttc aatcagacgt gaagctacct gcgggttacg gctgttgagg tcggtaagca
     4501 tttccaccag gaactggtaa ccgctgccat cttcggcatg gaacgctgcc gggttgctgc
     4561 ccgcaaacgc accaatcaac gaacgaatac ggttcgggtt gctcatggta aatgagcgat
     4621 gctgcaacag accacgcacc gtctccagca cattcgccgc cgggctggtg gcttgcagga
     4681 taaaccattt atccatcacc agaccgtcct gatgccactt atcgtcgtac tcctgcatca
     4741 gcgcgtcacg gcaaggcagc tgtgcagcaa ccgccgcaga aagcgctgcc agcgcatcgg
     4801 tcatattgtt tgcttcatgg tattgcttgc tcaccagcac gtcagccaga tgcgtttcac
     4861 caaaagcaag gaagcgcagg caggcattac gcagagtgcg ttttgcaata tcttcatgct
     4921 caacacggta ctcgctctgg taattcgcgt tgtaaatagc cagcagctca tccgccagtt
     4981 cagtcgccag agtacgagtg agtgcttcgc gtacttcggc aatagcaatc gggtcgatga
     5041 tatcgaacag ttcagccatt tcattgacag aaggcagcgt caggatttct gccgccagcg
     5101 ccggatcaat cttctcatcg agcagcaccg cgcggaaagc gtcagccaca tgtaccggca
     5161 gagacagcgg ctgcccttgc tgatgacggg cgacgttcag cttgatgtag gttgccagca
     5221 aactttgcgc cgcatcccaa cgggagaaat cattacgcgc atgacgcatc aggaaggtca
     5281 gttgctgatc gctccactta tattccagtt tcactggcgc agagaattcg cacagcagcg
     5341 caggcaccgg ctggaagtag acattatcaa aaacaaaggt ctgttccgcc tgggtgacgt
     5401 tcagcacgga attcaccgga tgaccgcctt tctgcaacgg gatcactttg ccttcgttgt
     5461 catacaattc gatggcaaat ggaatatgca gcggctgttt ttctgcctga tccggcgtgg
     5521 ctggcgtgcg ctggctgatg gtcagggtgc actgctcggt ttccggattg tagtcgtctt
     5581 tgacggtcac aatcggcgtg ccggactggc tgtaccaacg gcggaaatgg gagagatcga
     5641 cattcgacgc atcttccatc gcctgcacaa agtcgtcgca ggttgccgcg ctgccatcat
     5701 ggcgctcgaa atagagttgc atccctttct ggaagttttc ttcgcccagc agggtgtgga
     5761 tcatgcgaat cacttccgcg cccttctcgt aaacggtcag ggtgtagaag ttgttcatct
     5821 caatgaccat atccgggcgg atcgggtgcg ccatcgggct ggcgtcttcc gcaaactgca
     5881 atccgcgcat ggtgcgcaca ttattgatac ggtttaccgc gcgggaacca aggtcagagc
     5941 tgaattcctg atcgcggaag acggttaaac cttctttcag gctgagctgg aaccagtcgc
     6001 ggcaggtcac tcggttaccg gtccagttat ggaaatattc atggccgata acgcgttcaa
     6061 tatcgaggta atctttgtca gtggcggtgt cggtgcgggc cagcacatat ttggagttaa
     6121 agatattcag ccccttattc tccattgcgc ccatattgaa gaaatccacc gcgacgatca
     6181 tatagatgtc gaggtcatac tccaggccga agcgttcttc atcccatttc attgagtttt
     6241 tcagcgaggt catcgcccac ggcgcgcgat caaggttgcc gcgatcgacg tacagctcca
     6301 gcgccacttc gcgaccagaa cgcgtggtaa aggtgtcgcg cagtacatca aagtcgcctg
     6361 ccaccagcgc aaacaggtag cacggtttcg ggaacgggtc ctgccactgt acccaatggc
     6421 gtccgttttc cagttcacct tgcgcaacgc ggttaccgtt ggaaagcagg aagggatatt
     6481 tggttttatc ggcaataatt ttggtggtaa aacgcgccag cacgtccggg cggtcgagat
     6541 aatacgtaat atggcggaaa ccttcggctt cacactgggt gcaaagcgca tcgcctgact
     6601 gataaagccc ttccagcgcc gtattcgccg ccgggcttat ttcattaatg atcttaagcg
     6661 taaaacgctc cggcaaatta ctgatgacca gtgcgccctc ttcttctttc caggcggtcc
     6721 acggctcatc attaatatga acagaaacca gtttgaggtc ttcgccgttg agacgaagcg
     6781 gagcatctga tgcaccatga cggacagcct ggctgaccgc ggtaacgacc gtcttttgcg
     6841 cgtcgaggtc aaaggtcaag tcaatatcag taatctggta atccggcgca cgataatcgt
     6901 gacggtattt ggcttgtggc tgttgagtca taaataacct ttagcatctt ttatagagtc
     6961 tggtgttcag tctattcctg ttgcgtaaat cgcgctatgc agaatcttca tcttttcagg
     7021 tacaaacgcc tttattgcta catttttata acatacaccg cgtaatgcca tcgaccagaa
     7081 aggtggcata tggtgtgatc ggggttcaat aaattgcgaa acaaggtata ctccagcagt
     7141 tcctgaagat gtttattgta ctaaacgctc ctgtacgagg acgctactgc gcacctatga
     7201 cacaattcgc ttctcctgtt ctgcactcgt tgctggatac agatgcttat aagttgcata
     7261 tgcagcaagc cgtgtttcat cactattacg atgtgcatgt cgcggcggag tttcgttgcc
     7321 gaggtgacga tctgctgggt atttatgccg atgctattcg tgaacagatt caggcgatgc
     7381 agcacctgcg cctgcaggat gatgaatatc agtggctctc tgccctgcct ttctttaagg
     7441 ccgactatct taactggtta cgcgagttcc gctttaaccc ggaacaagtc accgtgtcca
     7501 acgataatgg caagctggat attcgcttaa gcggtccgtg gcgtgaagtc atcctctggg
     7561 aagttccttt gctggcggtt atcagtgaaa tggtacatcg ctatcgctca ccgcaggccg
     7621 acgttgcgca agccctcgac acgctggaaa gcaaattagt cgacttctcg gcgttaaccg
     7681 ccggtcttga tatgtcgcgc ttccatctga tggattttgg cacccgtcgc cgtttttctc
     7741 gcgaagtaca agaaaccatc gttaagcgtc tgcaacagga atcctggttc gtgggcacca
     7801 gcaactacga tctggcgcgt cggctttccc tcacgccgat gggaacacag gcgcacgaat
     7861 ggttccaggc acatcagcaa atcagcccgg acctagccaa cagccagcga gctgcacttg
     7921 ctgcctggct ggaagaatat cccgaccaac ttggcattgc attaaccgac tgcatcacta
     7981 tggatgcttt cctgcgtgat ttcggtgtcg agttcgccag tcggtatcag ggcctgcgtc
     8041 atgactctgg cgacccggtt gaatggggtg aaaaagccat tgcacattat aaaaagctgg
     8101 gaattgatcc acagagtaaa acgctggttt tctctgacaa tctggattta cgcaaagcgg
     8161 ttgagctata ccgccacttc tcttcccgcg tgcaattaag ttttggtatt gggactcgcc
     8221 tgacctgcga tatcccccag gtaaaacccc tgaatattgt gattaagttg gtagagtgta
     8281 acggtaaacc ggtggcgaaa ctttctgaca gccctggcaa aactatctgc catgataaag
     8341 cgtttgttcg ggcgctgcgc aaagcgttcg accttccgca tattaaaaaa gccagttaat
     8401 atcatcaggg agctaatcgg ctcccttttt ttacctttaa ttccgaaatc tttcgctgca
     8461 tttgcgaatt ctgcttgtct gattccagat gccaggtaac ataggtatcc ccccatttac
     8521 gggtggacaa gtgtttattt tttccgacta ttaacagaga gaatattatg agcgttgtgc
     8581 ctgtagccga cgtactccag ggccgcgtag ccgttgacag cgaagtcacc gtacgcggat
     8641 gggtacgtac ccgccgagat tcaaaagctg gcatctcctt cctcgccgtt tatgacggtt
     8701 cctgctttga tcctgtacag gctgtcatca ataattctct gcccaattac aatgaagacg
     8761 tcctgcgtct gaccaccggc tgctcggtca ttgtgacggg taaagtcgtg gcgtcgccgg
     8821 gccaggggca acaatttgaa attcaggcca gcaaagttga agttgctggt tgggttgaag
     8881 atccagacac ttacccgatg gcggcaaaac gccacagcat tgagtatctg cgtgaagtcg
     8941 ctcacctgcg tccgcgcaca aacctgattg gtgccgtcgc gcgcgttcgc catacgctgg
     9001 cgcaggcgct gcatcgcttc tttaacgagc agggattctt ctgggtttca acgccactga
     9061 ttaccgcatc tgataccgaa ggtgcaggcg aaatgttccg cgtttctacg ctggatctgg
     9121 aaaacctgcc gcgtaacgat cagggcaaag tggatttcga caaagacttc tttggtaaag
     9181 agtctttcct gaccgtatct ggccagttga acggcgaaac ctacgcttgc gcattatcca
     9241 aaatttatac cttcggcccg actttccgtg ctgaaaactc caacaccagc cgtcacctgg
     9301 cggaattctg gatgctggag ccggaagtgg cgtttgctaa cctgaacgat atcgcgggtc
     9361 tggctgaagc catgctgaaa tatgtcttca aagcggttct cgaagaacgc gcagacgaca
     9421 tgaaattctt cgctgaacgc gtagataaag atgccgtttc acgtctggaa cgcttcattg
     9481 aggccgattt cgcgcaggtg gattacaccg acgcagtgac cattctcgaa aactgcggca
     9541 ggaagtttga aaacccggtt tactggggcg tcgatctctc ttctgagcat gagcgttatc
     9601 tggcggaaga acactttaaa gcaccggtag tggttaaaaa ctatccgaaa gatattaaag
     9661 cgttctatat gcgcctcaac gaagacggta aaaccgttgc ggctatggac gttctggctc
     9721 cgggcatcgg tgagatcatt ggtggctccc agcgtgaaga acgtctggac gttctggacg
     9781 agcgtatgct ggaaatgggc ctgaataaag aagattactg gtggtatcgc gatctgcgtc
     9841 gctacggtac tgttccgcat tcaggtttcg gtcttggttt tgaacgcctg attgcttacg
     9901 taactggcgt gcaaaacgtg cgtgatgtga ttccgttccc acgtactccg cgcaacgcca
     9961 gcttctgatt ttcttcctca aagagccagc gcctgctggc tcttttttat cctttctgtc
    10021 aagttatctg tttgttaagt caagcaatct atttgcaacc ccgccataac gatcatcctg
    10081 ttacggaata ttacattgca acatttacgc acaaaaaata acccgcattc ttat
//
LOCUS       DAJOWB010000147        10058 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJOWB010000147 DAJOWB010000000
VERSION     DAJOWB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.147"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            180..683
                     /locus_tag="OHO85_004109"
     CDS             180..683
                     /locus_tag="OHO85_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCQ2673410.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(762..1013)
                     /locus_tag="OHO85_004110"
     CDS             complement(762..1013)
                     /locus_tag="OHO85_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCQ2673411.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1128..1214)
                     /gene="azuC"
                     /locus_tag="OHO85_004111"
     CDS             complement(1128..1214)
                     /gene="azuC"
                     /locus_tag="OHO85_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCQ2673412.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1477..1800
                     /locus_tag="OHO85_004112"
     CDS             1477..1800
                     /locus_tag="OHO85_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCQ2673413.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1971..2468
                     /gene="ftnA"
                     /locus_tag="OHO85_004113"
     CDS             1971..2468
                     /gene="ftnA"
                     /locus_tag="OHO85_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCQ2673414.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2506..2745)
                     /locus_tag="OHO85_004114"
     CDS             complement(2506..2745)
                     /locus_tag="OHO85_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCQ2673415.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            2935..4146
                     /gene="tyrP"
                     /locus_tag="OHO85_004115"
     CDS             2935..4146
                     /gene="tyrP"
                     /locus_tag="OHO85_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCQ2673416.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4208..4873)
                     /gene="yecA"
                     /locus_tag="OHO85_004116"
     CDS             complement(4208..4873)
                     /gene="yecA"
                     /locus_tag="OHO85_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCQ2673417.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEDNFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5069..5155)
                     /locus_tag="OHO85_004117"
     tRNA            complement(5069..5155)
                     /locus_tag="OHO85_004117"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5119..5121),aa:Leu,seq:taa)
     gene            complement(5168..5241)
                     /locus_tag="OHO85_004118"
     tRNA            complement(5168..5241)
                     /locus_tag="OHO85_004118"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5207..5209),aa:Cys,seq:gca)
     gene            complement(5296..5371)
                     /locus_tag="OHO85_004119"
     tRNA            complement(5296..5371)
                     /locus_tag="OHO85_004119"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5336..5338),aa:Gly,seq:gcc)
     gene            complement(5523..6071)
                     /gene="pgsA"
                     /locus_tag="OHO85_004120"
     CDS             complement(5523..6071)
                     /gene="pgsA"
                     /locus_tag="OHO85_004120"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCQ2673418.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6128..7960)
                     /gene="uvrC"
                     /locus_tag="OHO85_004121"
     CDS             complement(6128..7960)
                     /gene="uvrC"
                     /locus_tag="OHO85_004121"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCQ2673419.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7957..8613)
                     /gene="uvrY"
                     /locus_tag="OHO85_004122"
     CDS             complement(7957..8613)
                     /gene="uvrY"
                     /locus_tag="OHO85_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCQ2673420.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8909..9085
                     /locus_tag="OHO85_004123"
     CDS             8909..9085
                     /locus_tag="OHO85_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673421.1"
                     /translation="MIKIFIGHYINAFNSTVDDTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            9072..9296
                     /locus_tag="OHO85_004124"
     CDS             9072..9296
                     /locus_tag="OHO85_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCQ2673422.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9364..>10058)
                     /gene="sdiA"
                     /locus_tag="OHO85_004125"
     CDS             complement(9364..>10058)
                     /gene="sdiA"
                     /locus_tag="OHO85_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCQ2673423.1"
                     /translation="RTMLLRFQRMEAAEEVYHEIEFQAQQLEYDYYSLCVRHPVPFTR
                     PKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSEAQPLWEAARA
                     HGLRRGVTQYLMLPNRALGFLSFSRSSAREIPILSDELQLKMQLLVRESLMALMRLND
                     EIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNMQKKINAPNKT
                     QVACYAAATGLI"
BASE COUNT         2524 a         2528 c         2321 g         2685 t
ORIGIN      
        1 cttgatgatt taagcatttt cttatacccg ttcagacgtt attcttattt cagatcatcg
       61 tcagaattga ctccacgatc acatttcgga ccggcagaaa ggaattattc tgcaaacagt
      121 aattatggtg ttttgattta tcttgcacct ctccacttct ggatataagg atattaggta
      181 tggcaaccgc tggaatgctt ctcaaactca actctcaaat gaaccgcgag ttttacgcat
      241 ccaatctcta ccttcacctg agtaactggt gttctgaaca gagtctgaac ggcaccgcca
      301 ctttccttcg cgcccaggca cagagtaatg tgacccaaat gatgcgcatg tttaacttta
      361 tgaagagtgt cggcgctacc cccatcgtta aagccattga tgttcccggt gaaaaactga
      421 actctctgga agaactgttc caaaaaacga tggaagaata cgagcaacgt tctagtacgt
      481 tggcacagtt agccgatgaa gcgaaagaac tgaatgatga ttcaaccgtc aatttcctgc
      541 gcgatctgga aaaagaacag cagcatgatg gtctgttgct gcaaaccatt cttgatgaag
      601 tgcgcagtgc gaaacttgcg ggtatgtgcc ctgtgcagac cgaccaacat gttctgaatg
      661 tcgtgtcaca ccagctgcat tgatcatcat cggcgctaat gcattgcgcc gatgaaggtt
      721 ttgagaaacc gctgcctcat ctgtttgaag cagcggtttt tttaatggga ttcaccctgt
      781 ggggtaaact tgagttcaat aagcgcgatg gctttttgga ttgcccgcat ggtgaccggg
      841 tctgcggcgg cgggatggtt agtaaagtcg atattcttca gctgactgga cattttttca
      901 cgaacttcaa cgggcgcgat tacatcgaga acatccagaa tttgtttgat aaccaactgg
      961 caagcaacca catcagaaac cagttcctga tcggcattca gcggctggga catcgtaaac
     1021 tcctgatagc attttgaaag ccgttatagt agcggcttca catcttcagc gatagtcaca
     1081 tccaccgtca tcaggacaca aaaaaacctg ccggagcagg ttttttgtta tcggaacata
     1141 ttgcctggcg gtacgtcttt gaacgtcttg caatagttat tgaacatact tttcaggatt
     1201 ttgcgcagtt tcatcgcggc actccgacta tttgttatac aggtgttatt gtctttgcgc
     1261 ttataatatg acaaccatca caaaaatcaa tctttatgtg atgcaaatca cataaatacc
     1321 cctttaatgt tataaaaatg ataatcaaaa aacagccccc ctatttctga cacctacaga
     1381 tggcaagaaa tagcgcctgc caggcgtctt ttccggccat tgtcgcagca ctgtaacgcg
     1441 taaaatagtg ctttctctta ctcttctggc tggaccatga gacttctgat tctgactctt
     1501 tcattaataa cgctggcggg gtgtacggtt actcgccagg cccatgtgag cgaagttgac
     1561 gcggcaacag gtattgtacg gttagtttat gaccaggctt ttttgcagca tgcccatacc
     1621 gatcgttatg tcagtcgcgg gatcgccgat cgcgcatgcc agcaggaagg ctatacccac
     1681 gcggtcccct ttggtcagcc agtaggcaac tgtagccttt ttgcgggttc cttatgtttg
     1741 aatacggaat tcactttgtc atatcagtgc catcattccg ccttccctgt ctttctctaa
     1801 atattttaat cagcgagggg atcttcgctg attaaagaaa tagcggtaat gcgttttatt
     1861 cccattcgta tttttaataa ttgaagttta tattttacct tttgcaaata ataaaataac
     1921 aaattatagt gacgccacag caacaaatat aacctttgtg gagcactatc atgctgaaac
     1981 cagaaatgat tgaaaaactt aatgagcaga tgaacctgga actgtactct tcactgcttt
     2041 atcagcaaat gagcgcctgg tgcagctatc ataccttcga aggtgctgcc gcgttcctgc
     2101 gccgtcacgc ccaggaagag atgacgcata tgcagcgtct gtttgattac ctgactgata
     2161 ccggcaattt accgcgtatt aataccgttg aatctccgtt tgctgaatat tcctcacttg
     2221 atgaattatt ccaggaaacc tataaacacg aacaattaat cacgcagaaa attaacgaac
     2281 tggctcatgc tgcaatgacc aatcaggact acccaacatt taatttcctg caatggtatg
     2341 tttctgagca gcatgaagaa gagaaactgt tcaaatcgat tattgataaa ttaagcctgg
     2401 caggcaaaag cggcgaaggt ctgtatttta tcgacaaaga actctctacc ctcgacacac
     2461 aaaactaatg ctctcaggcg gcaggttttc tgccgccgtt tcaaatcagt gacggcaaat
     2521 cttactctga tcggtcgaaa aaccttcttc tgaaggtata aatttgcctt ttgctgccag
     2581 aaacgctacc agctctcccg cagtcatccc ttctgccgag caggtgtgaa aacgtgcctg
     2641 ttcaccaaaa cgcgctttaa ttgctgcttc cagactggca tgcgtatatt gctcgcctga
     2701 ttcaatcatc atatttaaca cttcatgacc gtgaatagag tccatcgttc ctcctcaaaa
     2761 aaagcctagc gtagcgattg ccgcttatga agactttgcg ccagcgcaga actaaacgct
     2821 tttattgtac atttatattt acactatatg taacgtcggt ttgacgaagc agccgttatg
     2881 ccttaacctg cgccgcagat atcactcata aagatcgtca ggacagaaga aagcgtgaaa
     2941 aacagaactc tgggaagtgt ttttatcgtg gcgggaacca caattggcgc aggcatgctg
     3001 gcaatgccgc tggctgcggc cggtgttggt tttagcgtta cgttaatctt gttgattggg
     3061 ctttgggcgt tgatgtgcta cacggcgcta ttactgctgg aggtgtacca gcatgttccg
     3121 gcagataccg gtcttggcac gctggcaaaa cgctatctgg gacgctacgg tcagtggctg
     3181 acgggcttca gtatgatgtt cttaatgtat gctctgactg cggcatacat cagcggtgcc
     3241 ggtgaattgt tggcctccag catcagcgac tggacaggta tttcaatgtc ggcaaccgct
     3301 ggcgtgctgt tgttcacttt tgttgccggt ggcgtggttt gtgtcgggac atcgctggtc
     3361 gatttgttta accgttttct gttcagcgcc aaaattattt ttctggtggt gatgctggta
     3421 ttgctgctac cgcatattca caaagtgaat cttttaaccc tgccgttgca acaggggctg
     3481 gctctgtccg caatcccggt gatttttacc tcgtttggtt ttcacggtag cgtgccgagt
     3541 attgtcagct atatggatgg caacgttcgt aagctacgct gggtgtttat aaccggtagt
     3601 gcgatccccc tggtggcata tattttctgg caggtggcga cgcttggcag cattgattca
     3661 acaaccttta tgggattgct ggctaatcat gccggattaa acgggctgtt acaggcgtta
     3721 cgcgaaatgg tggcctctcc gcatgttgag ctggcagtgc atttatttgc tgatttagcc
     3781 ctcgccacgt catttctcgg cgttgcgtta ggcttatttg attatctggc tgatttgttt
     3841 cagcgttcaa ataccgttgg tggacgattg caaactggtg caattacctt tctgccgccg
     3901 ttggcgtttg cactgtttta tccacgagga tttgtgatgg cgctgggtta cgccggtgtg
     3961 gcgctggcgg tactggcatt gattatccct tcactattga cctggcaaag cagaaagcac
     4021 aatcctcagg cgggttaccg ggtgaaaggt ggtcgtccgg cgctggtggt ggtgtttctc
     4081 tgtggtattg ctgtgattgg cgtgcaattt ttgattgcgg cagggttgtt accagaagtg
     4141 gggtgatcag atagcctcaa attccttatt gggtgccaga attaacgctg acacccaatt
     4201 tgacttctta atgcaggcag cactgcttaa atttcttacc actgccgcac ggacaaggat
     4261 cgttacgccc cggtttctct tctgctttga tcggttgctg aacagctttt tcctgcggat
     4321 gcgccatcca gtatgcatgt aaatcaagcg ccgccagtcg aatggcatct acgctctctt
     4381 caaacgcttc tggcgacatc ttctctaccc gctcgaagtt gtcctcagta ccgtgcagcg
     4441 ctatcgcctc cagcgctggt tttaacgaat cgggcaatgt tgaccagtca gaaagtgcca
     4501 cgccccgcat atagccaaag caccactcct caacaatcgt cagctcgctg ccatcaactt
     4561 ctcgcaagcc gaatagcggc tcaaactgct ccgggaattc gttcagacgc tctgcggtat
     4621 cggccatatg ttgaaaggcc aggttcataa agcgtgtcat ctctttctca gacgcccagc
     4681 gcggcacata gtcagcccca ccccacacgg caaccagcca ctgtgccggt tcaatctctt
     4741 gcggagaact caacaccgcc gtcaataaac cgtccagctc cgccacatca aggatggcgt
     4801 ggtcagtgtt gtatttggtc agaatatcgt ccagccattc caactcactt tcgtttaacg
     4861 gtcccgtttt catacgcttt tccttgtgga tctcaactcg ccagcaccta tcttacatgc
     4921 cggtccgtat cagagatact ttttgagtgg ctttgccggt gattaaaaat taaggagggt
     4981 gtaacgacaa gttgcaggca caaaaaaacc acccgaaggt ggtttcacga cactgcttat
     5041 tgctttgatt ttattcttat ctttcccatg gtacccggag cgggacttga acccgcacag
     5101 cgcgaacgcc gagggatttt aaatcccttg tgtctaccga ttccaccatc cgggctcggg
     5161 aagaaagtgg aggcgcgttc cggagtcgaa ccggactaga cggatttgca atccgctaca
     5221 taaccgcttt gttaacgcgc caaattcttc aggcctttca gccagacatc cgcttgaggc
     5281 cgatgtcttt taaactggag cgggaaacga gactcgaact cgcgaccccg accttggcaa
     5341 ggtcgtgctc taccaactga gctattcccg cattcatcaa gcaatcagtt aatcacttga
     5401 ttttattatc gtctggcaat cagtgccgcc gttcgatgcg ttgcattcta cttacctggc
     5461 gcgatgagtc aacgatattt ttcaccactt ttgatcgttt gctgaaaatt gcaccgaaac
     5521 gatcactgat caagcaaatc tgcacgcgca gcgctcaaat attgcaacat tgaccacaga
     5581 gtcagtaccg cagccacaaa gaaaagtgca ataccggcgt actcaaccca aatgttcgga
     5641 cgccacagca gccaggccaa cgccaccatc tgggccgtag ttttcacttt cccaatccag
     5701 gagacagcca cgctactgcg tttacccaac tccgccatcc attcgcgtag cgcagaaata
     5761 ataatttcac gggcgatcat cgttgccgcc ggtaaggtca cccaccagct gtgataatgc
     5821 tcggttacca gcaccatggc gatagccacg agaactttat ctgcgacagg gtcaaggaaa
     5881 gcaccaaacc gggtactctg gttccagcgg cgtgccagaa aaccatcgaa ccagtcagtc
     5941 accgccgcga cgcagaaaat gagcgcggcg gcaaacggcg accaggtgac aggcagataa
     6001 aagaccaata caaagaatgg gataaggatg acacggaaca gtgtaagcaa cgtagggata
     6061 ttaaattgca taatgacggg taactatctg ttgtcagtaa gattacccct atgttgctac
     6121 agagacatca atgtttcaac gaccagaaga tcttttctgc cagaccttgc gaaatacccg
     6181 gcacttttgc aatttcctcg acgctggcgt tacgtaaacc ttgcaaaccg cccatatatt
     6241 tcaacaacat ttgccgacgt tttggcccga cgccttcaat ggtttccagg gaactggtat
     6301 ttttgacctt cgcccgtttt ttacggtgcc cgccaatcgc gtgatcgtgt gattcatcgc
     6361 gaatatgctg gataacatgc agcgcgggag aatctggcgg caaactaaat ccctcacctt
     6421 ccggctcaaa gaacagcgtt tccagcccag ccttacgatc tgctcctttg gcaacgccaa
     6481 gtagcagcgg atgattttta tcccatgaga catccagttc ggcgaagaca tttttcgcct
     6541 gcgcaagctg gcctttgccg ccgtcgataa ggatcacatc cgggatctta ctgtcgtcaa
     6601 tggctttacc ataacgccga cgcagcacct gattcatcgc cgcataatca tcgcccggcg
     6661 tgatgccagt aatgttatag cgccgatact ccgcacgcag cgggccgtta gcatcaaaca
     6721 ccacacagga agcgacggtt tgttcgccca tggtatggct gatgtcaaag cactccatcc
     6781 gcttcacttc cggcaatttc aacacgctgg caagcgcggt cagtcgctgg tgaacggtag
     6841 attgctgcga aagtttgctg gttaaggccg tcgccgcatt ggtgcgcgcg agtttcagat
     6901 aacgcgccct atcgccgcga ggtttggttt gaacattaat cttgcgtccc gccagttctg
     6961 aaagggaatc ggcgagcagc gttttatcgc taagattaaa atcgagcagg atctcacccg
     7021 gtaaggtgcg catctggctg ccttgtaaat agaactgacc tacgaaggtt tccaccacct
     7081 cgctcagttc cgtaccgcca ggcactttcg ggaaatagct gcggctgccg agcactttgc
     7141 cctgacgaat gaacaatacg tggacacaag ccatgcccgc atcgaacgcc acaccaataa
     7201 cgtcaaggtc gtcgccggta ttggaaacaa actgtttttc ggtgacgcgt cgcaccgcct
     7261 gaatttggtc gcgaatacgt gcagcttctt caaactccag attctggctg gcagtttcca
     7321 tacggctaat cagttgcgta agcacctgat catctttgcc agacaaaaac aggcgcacat
     7381 actcgacctg ctgagcgtat tcttcttcac tcaccagtcc ttcaacgcac ggccccagac
     7441 aacgtcctat ctgatattgc agacacggac gcgagcgatt gcgataaaca ctgttttcgc
     7501 actggcgaat ggggaaaatc ttttgcagta gcgccagtgt ttcacgtacg gcatagccat
     7561 tcgggaacgg gccgaaatat tcacctttag catgcttcgc accacgatgc atcgccagac
     7621 gcggatgggt atcgccactc aggaagataa agggataaga tttatcatcg cgtagtaaaa
     7681 cgttgtaacg cggctgatag agtttgatgt agttgtgttc cagcaacagc gcttcggttt
     7741 ctgtatgagt aaccgttaca tcaatttgct ggatctgggc gaccagcgct tcggttttgc
     7801 gcgaagcgag gttgctacgg aaatagctgg aaagccgttt tttcaggtct ttcgctttgc
     7861 cgacatagat aaccgtacca ccagcatcgt acatgcgata aacgcctggc tggctggtta
     7921 cggtttttaa aaacgctttt gcgtcaaact gatcactcac tgacttgata atgtctccgc
     7981 attacacaga ccatggcgaa ttgccaggtg agtcagctca acatcgccat gaatgtttag
     8041 tttactgaac atacgatagc ggtagctgtt caccgttttc ggactgagat tgagctgttc
     8101 tgagatctca ttgaccttct ggcccttggt gatcatcagc ataatctgca attcacgttc
     8161 agacaaactg gcaaatgggc tttctgtttt ttctggttcg atctggctta acgccatttg
     8221 ttgagcgata tcagaagcaa tgtaacgctg ccctgaatag acagaacgaa tcgcactcac
     8281 gacttcctgc ggagccgcgc ctttgctgag gtagcccgca gcaccggcct gcatgacttt
     8341 cgctggtaaa gggttttctg tatggacggt aagcatgatg attttgacat cagctgtgga
     8401 acgcgcgatt ttacgcgtcg cctcaagacc gccaatgccc ggcatactca tgtccattag
     8461 caccacgtca acggcatttg tccggcacca cttaacggcg tcttcaccgc acgatgcctc
     8521 accgacgact tttataccct ttatatcttc cagaatgcgt cgtatccctg cgcgcaccag
     8581 ttcgtggtca tcaacaagta gaacgttgat caaaggaata tctccagaaa tagggataac
     8641 gctactgata gttagtcatt cgtatattag cggtttttat tgcaactttg aaacgttaaa
     8701 aatggtgggt attcgatttt tctctcgttt ttgtcaattc gattgtccac agttatggtc
     8761 acgcccgtca ccaacggctg accggatcgt ttcacagtaa gccctgcgac gaaatgtgac
     8821 aaaaattacc tttattcagc aaaaatgaaa atcagcccgc tgaatatgta acattaatta
     8881 acccaaacaa tacgggcaaa aaacattcat gataaaaata tttattggtc attatattaa
     8941 cgcatttaat agcaccgttg acgatacgct caaaaaacaa ccactgctat ttttagcaaa
     9001 gcttatggta tactccgccg ccttaacatt tttcaccgca aattttcatt gcaacatgac
     9061 gaggaaaata aatgagtacg cctgattttt ctactgccga gaataatcaa gaactggcaa
     9121 atgaagtctc ctgcctgaaa gcgatgctga cgctgatgct gcaggcgatg ggacaagctg
     9181 acgcgggccg cgtgatgtta aagatggaaa aacagcttgc gctgatcgaa gacgaaaccc
     9241 aggctgcagt attttccaaa acggttaagc aaattaaaca agcctaccgt cagtaatata
     9301 aaaccggctg agagcgtgcc tttcagccgg tttttgcatc tggcacgcag aacagaaaag
     9361 agatcaaatt aagccagtag cggccgcgta acaggcaacc tgcgtcttat ttggtgcatt
     9421 aatttttttc tgcatgtttt tctggtggaa attgaccgta ttctcagaga ttgacaaaat
     9481 catcgctatc tctgctgatg tcttcccttc cgccgtccat ttcaaaattt ctttttcgcg
     9541 cttgctgaaa ttcatctctg gcgtcatcac tatttcatca tttaaacgca tcagagccat
     9601 cagactttcg cgcaccagta actgcatttt taattgcagt tcatcactaa gaatgggtat
     9661 ttcgcgcgcg ctgctacggg aaaaggacaa aaagcccagc gcccggtttg gcagcattaa
     9721 atactgagtg acaccgcggc gtaaaccatg tgcgcgcgcg gcttcccata acggctgtgc
     9781 ttcgctgaat aagtcatcat tccacattaa atggccctga ctaaagtttt cagggttcag
     9841 caccggatca attgcgagaa agttttttgc ctgataataa ctaacccacg cctcagggta
     9901 attggtgtag aaagccactt taggtcgcgt gaatggtacc gggtggcgaa cacataacga
     9961 atagtaatcg tactccagct gctgagcctg aaactctatt tcatggtaga cctcttctgc
    10021 ggcctccatc ctctgaaaac gcaacagcat cgtgcgac
//
LOCUS       DAJOWB010000148        10039 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJOWB010000148 DAJOWB010000000
VERSION     DAJOWB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.148"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            6..81
                     /locus_tag="OHO85_004126"
     tRNA            6..81
                     /locus_tag="OHO85_004126"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="OHO85_004127"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="OHO85_004127"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(468..1226)
                     /locus_tag="OHO85_004128"
     CDS             complement(468..1226)
                     /locus_tag="OHO85_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCQ2673424.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(1234..2337)
                     /locus_tag="OHO85_004129"
     CDS             complement(1234..2337)
                     /locus_tag="OHO85_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCQ2673425.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2347..3528)
                     /locus_tag="OHO85_004130"
     CDS             complement(2347..3528)
                     /locus_tag="OHO85_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCQ2673426.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3596..4621)
                     /locus_tag="OHO85_004131"
     CDS             complement(3596..4621)
                     /locus_tag="OHO85_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2673427.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5052..5273)
                     /locus_tag="OHO85_004132"
     CDS             complement(5052..5273)
                     /locus_tag="OHO85_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673428.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5526..8630)
                     /gene="acrF"
                     /locus_tag="OHO85_004133"
     CDS             complement(5526..8630)
                     /gene="acrF"
                     /locus_tag="OHO85_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HCQ2673429.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMEFGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVTRR
                     CFKG"
     gene            complement(8642..9799)
                     /gene="acrE"
                     /locus_tag="OHO85_004134"
     CDS             complement(8642..9799)
                     /gene="acrE"
                     /locus_tag="OHO85_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCQ2673430.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2672 a         2595 c         2409 g         2363 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aataaaacaa aaggctcagt cggaagactg ggccttttgt tttatttgat gtttgtcggt
      301 gaacactctc ccaaccagga caaatccgcc gggaacggat ttgaacgttg cgaagcaacg
      361 gcccggaggg tggcgggcag gacgcccgcc ataaactgcc agacatcaaa tcaagcaaaa
      421 aggccatccg aaaggatggc ctttttgctt ttcgaactaa cattcaatta atggattacc
      481 tgcgataaaa atgccctcgt acgctctgat ttaggatgcg taaaaaattc atcaggtgca
      541 gcttgctcca ctatttcccc acgatccata aaaatcaccc ggtcagcgac ggttcgcgca
      601 aaccccatct catgcgttac acacaacatt gtcatacccg attgcgccag cccaatcatc
      661 gtatccagca cctctttcac catctccgga tcgagcgccg acgttggctc atcaaacaac
      721 ataattttcg gcttcataca cagcgaacgc gcaatggcaa cgcgttgctg ctgaccacca
      781 gaaatttgtc cgggaaactt atgcgcatgt tcggcaattc tcacccgctc aaggtaatgc
      841 atcgccagag cttcagcctc tttctttggc atcttgcgta cccaaatcgg tgccagggta
      901 cagttctgta gaacggtcag atgagggaag agattgaaat gctgaaagac cattcccact
      961 tcctgccgga cgcgctcaat attgcggata tcttcattaa gttcgatgcc atctaccacg
     1021 atccgtccct gttgatgttc ttccagatga ttaatacaac gaatggttgt cgatttaccg
     1081 gaacctgaag ggccacacag aacgatccgt tctcccggtt gcacggttaa atttatattt
     1141 ttcaaaacat ggaattgtcc ataccattta ttgacgtttt ccagcgtaat catcgcgtta
     1201 gcaggttgca gtaaaatttg gctcatagtg tcctcaatgc ggtgtacgcc cggtgttaaa
     1261 acgtttttcc agatgctggc tatagcgcga catgctgaaa cagaagatcc agtagatcag
     1321 cgcggcgaaa acataccctt ccgtcgacat acccaaccag gtgggatcaa cggttgcctg
     1381 ctgaacgcta ctgaaaagat cgaacaaccc gataatgatc accaggctgg tatctttgaa
     1441 gagtgcgatg atggtattta ccagcccagg aattaccagc ttcaacgcct gtggcagaat
     1501 aaccagcccc tgagttttcc agtaacccaa cgccagcgac tctgccgctt catactgccc
     1561 tttaggcagt gcctgtaatc cgcctcgcac gacttccgca acatatgctg actgaaacag
     1621 gatcacgcca accagcgcgc ggatcagttt gtcgatactg gtgccttctg ccataaacaa
     1681 cggcagcatg accgaagaca taaacaatac ggtaatcagc ggtacgccgc gccagaactc
     1741 gataaaaatg accgataaga tacgcacaat cggcatatgg gagcggcgac ctaacgccag
     1801 taagatcccc cacggtagcg cccccgcaat cccaacggat gcaataatta aagttagcgt
     1861 cagcccgccc cattgccggg tttcaacccg ctccagacca aaaaaaccgc catacatcag
     1921 ccaccagaca atcagtgggt aaatcaccgc ccagaccgca atatagcgac cgcgatgcgg
     1981 gagtattttc cagaacattg gtgcgattga cacaaggcca atcagtaatg ccaggttaat
     2041 tcgccagcgt tggtcgtgtg ggtaaagtcc atacataaac tgaccaaatc gttcgtggat
     2101 gaagacccaa caggcaccgg cttttgtgca gtcggcacgc gttgagccaa cccaattagc
     2161 ctgcaaaaat gcccagttca gcaacggtgg gatcagttcc cacatcaacc atatgcagcc
     2221 aatagtcagc aggctattgc tccagctgga gaacagattt tttcgcaccc acaccatcgc
     2281 gcggcttgag ttatggctcg ctgggcgcga gggctgagac agcaatactt ttgtcatcat
     2341 gactccttag cgttcaacga tcgcgatgcg gcggttatag atattcatca gcagcgagat
     2401 agtcaggctg ataatcagat agaccgacat ggtcatggct atcgtctcga tggcttgccc
     2461 cgtctggttc agcacggtgc cagcaaacag cgaaaccata tcgggatagc caatagcggc
     2521 ggcaagagag gagtttttga cgatgttgag atactggctg gttaacggcg gaataatcac
     2581 tcgcagtgcc tggggaataa tgacctggcg tagcgtaacc gggttgggta atcccagtga
     2641 ccgagccgct tcatgttgac cataaggcac tgcctggatc ccagcgcgga taatctcggc
     2701 gataaatgca gatgtataaa ccgaaagtgc cagcgttaag gctgccagtt caggaattaa
     2761 aaccatcccg ccgcggaagt taaagcctcg tagggctggg acatcccagt gtagtgctgc
     2821 gccaaaaagc cattgcgcca gtaaaggcaa accaatgatc aacactgcgg cgatcggcca
     2881 ggtgcggcgc agttgtccgg tctttatctg gtatgtttta ttaaagcgga atagcccaac
     2941 agaaaggact atagccataa caacagccag gataaacgca ataaatccgt ctcccagctg
     3001 cggtgacgga atataaagcc cacgattgct caaaaaggcc agatcaaacg cgctcacagc
     3061 ctggcgtggt ccgggcaaat tgcgcaacac ggcaaagtac cagaagaaga tttgcagcag
     3121 cgggggaata ttacggaaga tctcaatata aattgtggaa agctttcgta gcagccagtt
     3181 atccgaaagt ctcgccagac cgataaagaa gcccaaaaca gaagcgaaca caatacacaa
     3241 tgcagaaacc agtagcgtat tgagtaagcc gacaataaaa acgcgtccgt aggtgtcgcc
     3301 ctgctggtaa tcgatcaaat gctggacaat accgaagcca gcgccgcgat ccagaaaggc
     3361 aaaacctgaa gtaatgccac gattattgag attcgttaca gtgttgtgaa ataaccaacc
     3421 aacaatgccg acaacagcaa caacggcaag gatctggaat aaccaggcac gaaccgtagg
     3481 gttggcaaag gagagtgagc ctttaacggt tgagcggcga tgagacatag aaacctcgga
     3541 aagcataacg ctgaagagag cgccgccgtc gcgacgctcg ttacctcaca ccgacttaac
     3601 gcacgggcgg tgcgtactga ataccgccgt tattccagag attattttgc ccacgtttaa
     3661 ttttcagcgg gctttctgaa cctacgttac gctcaaaaat ttccgagtag ttacccacct
     3721 gtttgatgat gttataggcc catttattat ccaacttcag atccttgccg taatcgcctt
     3781 ctttacccag cagatgtgcc atatcaggcg ttgctggatt agccgctttt tcatcaacgt
     3841 tctgggaatt gatgcccatc tcttcagcat tcagcatggc gaaaagcgtc cagcgtacaa
     3901 tcgagaacca ttcatcatcg ccacgacgaa ctaccggccc aagcggttct ttagagataa
     3961 cctccggtaa gacaatccat tcagctgggt tgcttaattt gatgcgcagg gcatacagtt
     4021 gcgactgatc cgaggccagc gtatcgcagc gaccagattc cagtgccttc gctgattcgt
     4081 cagagcgatc gaaagtcact ggtgtgtact tcatattgtt tgccttgaaa tagtcggcaa
     4141 cgttgagttc ggtatcagta cccgcctgaa tacagacggt agcgccatcc agttctttcg
     4201 cgctttttag ccccgcttta tcgtgcgtca ggaagccaat gccgtcgtaa taagtgacgc
     4261 cagtaaatgc cattcccatc ccggcatcgc gagatgaagt ccaggtcgta ttacgggaga
     4321 gcaaatccac ttcccctgac tgtaaagccg tgaagcgttc ttttgctgtg agtggggtat
     4381 atttcacttt cgtgtcgtca ccaaatacag cagcggcaac accacgacaa acatcaacat
     4441 caatacctga aaacttaccg tctgcatcgg cataagagaa cccaggtaat ccatcactga
     4501 tcccgcattg cacaaaacct tttttctgaa cggcatcaag cgtcgcgcca gcatgcgcct
     4561 gatttgcaac ggcaagcagc acgctggcgg cagccagtgt ggctatcatc atctttttca
     4621 taatgcttcc tgtgaggcga aaattatcgt tataggacgt ttgggaaacg tcttttacct
     4681 gtctgtggcc atcctcttat aagcaaaagt agtgccagct tttgcgcccg atttcgcgtc
     4741 gcaataagct gagtgtagac aggaaataat aaaaacagcg ccccgcaatg gtgcgaaaat
     4801 gaaacatgcg ccacattttg gcgcaaaata tttacgcgtg ttttactaag cagaataatg
     4861 attgttagcg tgagtatatt tctggtggga agtgtgaaag ttaaattgat gcgttatttc
     4921 gaaagcagcg aaataacgcg atataacatg ctaaatcagc agagaattag ccagcagaat
     4981 atagcaatat tttatcttat gtaaagatac tgtatcgtta aacagagcgc gaaactccgc
     5041 gccctgttca gttatttcga cagagcgatg atccccatcg tcaggccagc aacagccgct
     5101 gccccgccag caatagcccc agccgtttcc cagttatcct gagtcgtgcc tttcatacag
     5161 gtattggtgt gaaccagacg accctgatcg tcataaaccg gaacgcaggg cgaatcgtga
     5221 gcacagcccg ccagcaatgc ggtgagcaat gccacaggaa ttaatctttt catggtgtta
     5281 cctcagtaat aaacgctaac gcttaataag ttaactctct tattaacgag ctgaatgcca
     5341 ttttatcacc ggaggttata aatcttgcgg aatgaataat gacaaattat gtgaattgta
     5401 aaaatagtga agggagcggg aactatagaa agcttttaaa gagtgagcag aagaatatct
     5461 tattaatttt tatgggtttc actggaaata ataaaggcac ccgaaagcgc ctttatgttt
     5521 ctgatttatc ctttaaagca acggcgggtc accacaaaga acaccggtac gaagaagatt
     5581 gccagcaacg ttgcagagac cattcctccc attaccccga tacccacagc gttctgcgcg
     5641 ccactgccgg caccgttact gatagctagc ggtaatacgc cgagaataaa ggcgagagag
     5701 gtcatcagga taggacgcag acgcatacgt actgccatta gtgtcgcttc aacaacacct
     5761 ttaccctctt tctccatgag atctttagcg aactcaacaa tcaaaatagc gtttttggcc
     5821 gacaagccaa ttgtcgttag caagcccacc ataaagtaga cgtcattttt ttgattaaag
     5881 agtgtcgccg ccagcagcac gccgacaatc cctaacggca cgaccaacat aaccgagaca
     5941 ggaattgacc agctttcata gagtgcagca aggcacagga aaacaaccac aaaggaaatt
     6001 gctaccagtg cgggagcctg gtttcccgat aagcgttcct gatacgacat acccgtccag
     6061 tcataaccaa tgccagcagg taattttgac gcaaggtttt ccatcaacgc catggcatcg
     6121 ccggaactgg ttcctggcgc ggcttccccc tgaatctcca ttgacggcag accgttgtag
     6181 cgttccagtc gcggagagcc atacacccaa tgtgaagtgg taaaggccga gaatggcacc
     6241 atttcgccgt tggcgctgcg gacataaagt ttatcgacat cttctggcag catacggaat
     6301 ttggcatccg cctgaacata cacctttttc acgcgaccac ggtcgatgaa gtcgttaacg
     6361 taagtcccac ccaacgccgt tgaaatggtc tgattgatgt cagaaagtga aacacctaat
     6421 gcctgcgcct tttcctggtc aacttccagt ttaaactgcg cggtgtcttc caggccatta
     6481 ggacgcacgc tgactaagct ggcaggatgt tgcgccgcca taccaagcaa ctggttacgg
     6541 gcctgggtta gggcatcgtg acccagccca gcctgatcaa ttaactcaaa gtcgaaaccc
     6601 gttgccgtgc ccagttcaac aatggctggc atattgaatg gaatgacaaa accgtcgcgg
     6661 atcttgccaa attccatttt ggcacgatgg attaccgctt ccgcactgtt ttcatcacca
     6721 ttacgctctt cccacggttt cagactgacg aaggccatac cggcgttttg tgcctggccg
     6781 ctgaagctaa agccgttaac cgtaaagaca ctttcaacgt tcgctttctc gttcttcaga
     6841 tagtaatccg tcacttgatc caacactttt tgcgtccgct cttgcgtcgc gccagcgggt
     6901 aactgaatca tggtcagaaa gacaccctga tcctcttcag gtaagaagga agacggaaga
     6961 cgtaaaaaca acaccaccat tcctgcaaca atcagcgcat agatcagtaa atatcgtcct
     7021 gtggaaccga ggattttgcc gacgctgttg gtgtagtggt taacgctatg atcgaaggta
     7081 gtattaaacc agccgaagaa accgccctta ttttcgtgat gctcagcaga gacgggttta
     7141 agcagcgttg cacataacgc aggggtaaga atcaatgcca ccagaacaga aagcgccatt
     7201 gccgaaacga tggtgataga gaactggcga taaattgccc cagtagaacc gccgaagaat
     7261 gccatcggaa taaataccgc tgacagcacc atcgcgatac ccaccagtgc gccctggatt
     7321 tgcgacattg atttttccgt cgcttctttt ggcgggagct tatcctccat catcacgcgc
     7381 tcgacgttct ccaccaccac tatcgcatca tcgacgagca gcccgatggc aagcaccatc
     7441 ccgaacatcg ttagtgtgtt gatggagtaa ccaaaagcgg cgaggatggc aaacgtccct
     7501 aacaacacca cgggtaccgc aatggtgggg atcagcgttg ctcgcatatt ctgcaagaac
     7561 agatacatca ccaggaacac cagcataatg gcttcgaaca gcgtttttac cacttcgtga
     7621 atagaaagct ggacgaatgg cgtggtgtca taagggtaga gaaccttcat tccctgcggg
     7681 aagaatggct gtaattccgc cagttttgcc ttaatggctt tcgcggtatc gagagcattc
     7741 gcgccggttg ccagcttaat ccccaggccc gccgccggtt ttccgttgat acgagcgata
     7801 acgttatagt tttcaccgcc aagttcaacc cgtgcgacat ctttcaggcg taccaccgag
     7861 ccgtcactgt ttacgcgcag ggtcactttg ccgaattctt ccggattttt taaccgcgtt
     7921 tgagcaataa tcgaggcatt caactgttgc cctggtaacg ctggcgttcc gcccaactgt
     7981 ccggcagcga tctgatcgtt ctgtaccttc aactggttaa tcacatcaac cggtgtcagt
     8041 ttatatttgt ttagcagatc ggcgtccagc cagatacgca tcgcatactg tgcgccgaaa
     8101 agctgtacgt caccgacgcc attcagacgg ctaagcgtat ctttaacgtt agaggccaca
     8161 tagtccgaga tatcgtcctg tgtggtgtct gggttatcag agacaaagcc cgccaccatc
     8221 aaatagctgc tactggactt ttcaacactg atcccctgct gctgaacctc ctgcggcagc
     8281 aacggcgtgg cgagctggag tttgttctgc acctgcactt gcgcgatatc aggatcggtc
     8341 ccggactgga aggtaagggt aattgtcacg ctaccggcgg aatcgctggt ggaggacata
     8401 tacatcaggt tatcgatacc attcatattc tgttcgataa cctgcgtcac cgtatcctgc
     8461 acggtctgcg catcagcacc tggatagttt gccgaaacag aaaccgcagg cggtgcaatg
     8521 gttggatact gagcgacggg caattgtagg atcgccagtg cgcccgccat catcagaata
     8581 atggccagca cccatgcaaa tatcggtcgt cgaataaaaa agtttgccat gtcagattac
     8641 cttacttcga tgcagtatct gcgggggtat cggtagtggc tttaacctgc tctcccggac
     8701 gcgctttttg caggccgctg acaatgactt gatcgccaga tttcagacct tcgctaatca
     8761 accatttatc gccaatcgcc tgactggcaa cgacagggcg cgcttccacc tgacttttat
     8821 cgttaacaat cagcacggtt gcatcaccac gcggtgtgcg gctaacgcct tgttgcggga
     8881 taagaatggc gtcaggttgg acgccttcat caatccgtgc acgcacaaac atacccggca
     8941 aaagcgtatg ttgcgggtta gggaagacag cacgtagggt tatggagccg gtgctttcat
     9001 caacggtcac atcagagaat tgcagcgtac ctttcagggg ataggtttga ccgttttcca
     9061 tgaccaactc tacgttgctg gtggcgtttt ccttatgcaa atttccttgc tctacggatt
     9121 gcttcagcct cataaaatcg ttgctggatt gggtcacatc aacgtagata ggatcgagct
     9181 gctggacagt cgccagttca gtcgtttgcc cattagtgac aagagcgcct tcggtcacgg
     9241 tcgatttgcc gatacgtccg ctaattggcg cagtcacttt ggtataagcg agattgatgc
     9301 gagcgctttc gactgtggct tttgcggcaa tcacggcggc atcggcctgg cgagcatcag
     9361 caatggcctg gtcgtactcc tgctggctga tgtatttcgt acccacgagc ggaacgtaac
     9421 gttttaccgt caaatgcgcg atggcggcgg cggcttcact tttcgccagt tcgcctttcg
     9481 cgctgtcata atttgcctga taggtcgcgg gatcgatctg gtacagggac tggcctgctt
     9541 gtacatcgct gccttcagtg aaattacgat tcagtacgat cccgctaacc tgtgggcgaa
     9601 cttcggctat acggtaagca ttggtgcggc ctggtaattc agtcttaact tctaacgggg
     9661 ccgttttcac aatatgaacg gtaacctgcg gttcaccgac gtgagctttc tcttctccct
     9721 tatcgttaca accggcgatt aaagccgcgg agatcagaat aaaggagggc aggaggaaaa
     9781 acctggcatg tttcgtcatt actattcctc aaaaaaccaa aagcgcgtta tttacccaga
     9841 aaggcaacgc gttaactcgc agaaagaaaa atacagtttg ctatcctaca aattatcatt
     9901 cgtcgatgta aggaatagtt atgaatactg gcgtctcaag gcacataaac acaaaaaaag
     9961 attaatattc tactgtttta ttttgacgcg ggttgaaaga ggcagaatta aaacctcgta
    10021 aattgaaata tatattcat
//
LOCUS       DAJOWB010000149         9991 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJOWB010000149 DAJOWB010000000
VERSION     DAJOWB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.149"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            406..1383
                     /gene="csiD"
                     /locus_tag="OHO85_004135"
     CDS             406..1383
                     /gene="csiD"
                     /locus_tag="OHO85_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCQ2673431.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVSKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1403..2671
                     /gene="lhgO"
                     /locus_tag="OHO85_004136"
     CDS             1403..2671
                     /gene="lhgO"
                     /locus_tag="OHO85_004136"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCQ2673432.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAMELREREPNITGLGGIFVPSSGIVSYRNVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2694..4142
                     /gene="gabD"
                     /locus_tag="OHO85_004137"
                     /pseudo
     CDS             2694..4142
                     /gene="gabD"
                     /locus_tag="OHO85_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            4156..5436
                     /gene="gabT"
                     /locus_tag="OHO85_004138"
     CDS             4156..5436
                     /gene="gabT"
                     /locus_tag="OHO85_004138"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HCQ2673433.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGNFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            5674..7074
                     /gene="gabP"
                     /locus_tag="OHO85_004139"
     CDS             5674..7074
                     /gene="gabP"
                     /locus_tag="OHO85_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCQ2673434.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7095..7757
                     /gene="csiR"
                     /locus_tag="OHO85_004140"
     CDS             7095..7757
                     /gene="csiR"
                     /locus_tag="OHO85_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCQ2673435.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7710..8207)
                     /gene="kbp"
                     /locus_tag="OHO85_004141"
     CDS             complement(7710..8207)
                     /gene="kbp"
                     /locus_tag="OHO85_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCQ2673436.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEELLIASHRLLDN
                     GNWRQ"
     gene            complement(8291..8449)
                     /locus_tag="OHO85_004142"
     CDS             complement(8291..8449)
                     /locus_tag="OHO85_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCQ2673437.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8632..8931
                     /locus_tag="OHO85_004143"
     CDS             8632..8931
                     /locus_tag="OHO85_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2673438.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8941..9465
                     /gene="ygaP"
                     /locus_tag="OHO85_004144"
     CDS             8941..9465
                     /gene="ygaP"
                     /locus_tag="OHO85_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCQ2673439.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRHEQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPFPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9512..9916)
                     /gene="stpA"
                     /locus_tag="OHO85_004145"
     CDS             complement(9512..9916)
                     /gene="stpA"
                     /locus_tag="OHO85_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCQ2673440.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2295 a         2561 c         2718 g         2417 t
ORIGIN      
        1 caatattcct gggcgtagcg ctcttctttt agggaaaaca ggagaaccgc tgaactatct
       61 ctatttataa tttaatgtta tatctgcccc gataaaacgg ggcagataat atgtttagtt
      121 tactaacggt cattttgcag tgaagccatt tactgttttt tatcgaccag ataatctgtt
      181 ctctaatgtt aactctccct aacctgttgc tttagttatt catttcctgt ctcactttgc
      241 cttaataccc tacgttaaat gttactaatt tattgctttt gatcacaata agaaaacaat
      301 atgtcgcttt tgtgcgcatt tttcagaaat gtagatattt ttagattatg gctacgaaat
      361 gagcatcgcc atgtcaccct acatctcata agaggatcgc ttctgatgaa tgcactgacc
      421 gccgtacaaa ataacgctgt cgattcaggc caggactata gcggattcac tctcatcccg
      481 tcggcgcaat ccccgcgtct gctggaactc accttcaccg aacagacgac caaacagttt
      541 ctcgaacagg ttgccgaatg gcccgtgcag gcgctggagt acaaatcgtt tctgcgtttt
      601 cgggtaggca aaattcttga cgatctgtgt gcgaatcagc tgcaaccatt gctgttgaag
      661 accctgctaa accgcgctga aggtgcgctg ttgatcaatg cggtgggtat cgatgatgtg
      721 gcgcaggcag atgagatggt gaagctggca acggcggtag cgcatctgat tggtcgctca
      781 aatttcgatg cgatgagcgg tcagtattac gcgcgtttcg tggtgaaaaa tgtcgataac
      841 tcagacagct atctgcgtca gccgcaccgc gtgatggaac tgcacaacga cggcacgtat
      901 gtcgaagaga tcaccgatta cgtgctgatg atgaaaatcg atgagcaaaa catgcagggt
      961 ggaaattcgt tgctgctgca tctcgatgac tgggaacatc tggaccacta tttccgccat
     1021 ccactggcgc gtcgcccgat gcgctttgcc gcgccgccga gcaaaaacgt cagcaaagat
     1081 gttttccatc cagtgttcga cgttgatcaa cagggtcgcc cggtgatgcg ctatatcgac
     1141 cagttcgtcc agccaaaaga ttttgaagaa ggcgtgtggt tgagcgagct ttccgatgcc
     1201 attgaaacca gcaaaggcat tctttctgta cccgttcccg ttagcaaatt cctgttgata
     1261 aacaacctgt tctggctgca cggtcgcgac cgctttactc cgcacccgga tctgcgccgt
     1321 gaactgatgc gtcagcgtgg ctatttcgct tacgccactc accactacca gacgcatcag
     1381 taagcgcaaa ggaattgagc ggatgtatga ttttgtgatt attggcggcg gcatcatcgg
     1441 catgtcgacc gccatgcaac tgattgatgt ttatccggat gcccgcattg cgttgctgga
     1501 aaaagagtcc ggcccggcct gtcaccagac gggccacaac agcggcgtga tccatgccgg
     1561 ggtttattac acgcccggta gcctgaaggc gcagttttgc ctggcgggaa accgcgccac
     1621 caaagccttt tgcgatcaaa acggcattcg ctatgacaac tgcggcaaga tgctggtcgc
     1681 cacctctgaa ctcgaaatgg aacggatgcg cgcgttgtgg gaacgcacgg cggcaaacgg
     1741 tatcgagcgc gagtggttaa acgccatgga actgcgcgag cgcgaaccga atatcaccgg
     1801 gcttggcggc atttttgtgc catccagcgg cattgtcagc taccgcaacg tgacggcggc
     1861 aatggcaaaa atcttccagg ccagaggcgg tgagattatc tataacgccg aagtcagcgg
     1921 cctcagtgag cataaaaacg gcgtggtgat acgtacccgc cagggcggcg aatatgaagc
     1981 atcgacgctg attagctgtt ccgggctgat ggctgaccgg ctggtgaaaa tgctcggcct
     2041 cgaaccgggc tttattatct gcccgttccg cggcgagtat ttccgccttg cgccggagca
     2101 taaccagatt gttaaccatc tgatttaccc tattcccgac ccggcaatgc cgtttttggg
     2161 cgtgcatctc acccgaatga tcgatggcag cgtgaccgtc gggccaaacg cggtgctggc
     2221 ttttaaacgc gaaggctacc gcaagcgcga cttctcgttt agcgacacgc tggaaatttt
     2281 aggctcgtcg gggattcgcc gggtgctgca aaaccatcta cgctcaggac tgggcgagat
     2341 gaaaaactcg ctgtgcaaaa gcggctatct gcggctggtg caaaagtatt gtccccggct
     2401 ttcgttaagc gatctccagc cctggcccgc cggtgtgcgg gcgcaggcgg tatcgccgga
     2461 tggcaagttg attgacgatt ttctgtttgt caccaccccg cgcacgatcc acacctgcaa
     2521 tgcgccctcc ccggcagcga catcagcaat tcctattggt gcgcatattg tcagcaaggt
     2581 acaaacgctg ttggcaagcc agagtaaccc cggacgcacg ctgcgagcgg cacgtagtgt
     2641 ggatgcctta cacgccgcat ttaatcaata acctttgaaa acaggacgta acaatgaaac
     2701 ttaacgacag taacttattc cgccagcagg cgttgattaa cggggaatgg ctggacgcca
     2761 acaatggtga agtcatcgac gtcaccaatc cggcgaacgg cgacaagctg ggtagcgtgc
     2821 cgaaaatggg cgctgatgaa acccgcgccg ctatcgacgc cgccaaccgc gccctgcccg
     2881 cctggcgtgc gctcaccgcc aaagaacgcg ccaacattct gcgcaactgg ttcaatttga
     2941 tgatggagca tcaggacgat ttagcgcgcc tgatgaccct agaatagggc aaaccgctgg
     3001 ctgaagcgaa aggcgaaatt agctacgccg cctcctttat tgagtggttt gctgaagaag
     3061 gcaaacgcat ttatggcgac accattcccg gtcatcaggc cgataagcgc ctgattgtta
     3121 tcaagcagcc gattggcgtt accgcagcta tcacgccgtg gaacttcccg gcggcgatga
     3181 ttacccgcaa agccggtccg gcgctggcgg caggctgcac catggtgctg aaacccgcca
     3241 gtcagacgcc gttctctgcg ctggcgctgg cggagctggc gattcgcgcg ggcattccgg
     3301 ctggggtatt taacgtggtc accggttcgg cgggcgcagt cggtaacgaa ctgaccagca
     3361 acccgctggt gcgcaaactg tcgtttaccg gttcgaccga aattggccgc cagttaatgg
     3421 aacagtgcgc gaaagacatc aaaaaagtgt cgctggagct gggcggcaac gcgccgttta
     3481 tcgtctttga cgatgccgat ctcgataaag ccgtggaagg tgcgctgtcc tcgaaattcc
     3541 gcaacgccgg gcaaacctgc gtctgcgcca accgtttata cgtgcaggac ggcgtgtatg
     3601 accgctttgc cgaaaaatta cagcaggcgg tgagcaaact gcacatcggc gacgggctgg
     3661 agaatggcgt caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa gtggaagagc
     3721 atattgccga tgcgctggag aaaggcgcgc gcgtggtttg cggaggtaaa gcgcacgaac
     3781 gcggcggcaa cttcttccag ccaaccattc tggtggacgt tccggccaac gccaaagtgt
     3841 cgaaagaaga gacgttcggc cccctcgccc cgctgttccg ctttaaagat gaagccgatg
     3901 tgattgcgca agccaatgac accgagtttg gccttgccgc ctatttctac gcccgtgatt
     3961 taagccgcgt cttccgcgtg ggcgaagcgc tggagtacgg catcgtcggc atcaataccg
     4021 gcattatttc caatgaagtg gccccgttcg gcggcatcaa agcctcgggt ctgggtcgtg
     4081 aaggttcgaa gtatggcatc gaagattact tagaaatcaa atatatgtgc atcggtcttt
     4141 aactggagaa cgcgaatgag cagcaataaa gagttaatgc agcgccgcag tcaggcgatt
     4201 ccccgtggcg ttgggcaaat tcaccccatt ttcgctgacc gcgcggaaaa ctgccgggtg
     4261 tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg ggattgcggt gctcaatacc
     4321 gggcatctgc atccgaaagt ggtggctgcg gtggaagcgc agttgaaaaa actgtcgcac
     4381 acctgcttcc aggtgctggc ctacgagccg tatctggagc tgtgcgagat tatgaatcag
     4441 aaggtgccag gcaattttgc caagaaaacg ctgctggtca ccacaggttc tgaagcggtg
     4501 gaaaacgcgg tgaaaatcgc ccgcgccgcc accaaacgta gcggcaccat cgcttttagc
     4561 ggcgcgtatc acgggcgcac gcattacacg ctggcgctga ccggcaaggt gaatccgtac
     4621 tctgcgggca tggggctgat gccgggtcat gtttatcgcg cgctttatcc ttgcccgctg
     4681 cacggcataa gcgaggatga cgctatcgcc agcatccacc ggatcttcaa aaatgatgcc
     4741 gcgccggaag atatcgccgc catcgtgatt gagccggttc agggcgaagg cggtttctac
     4801 gccgcgtcgc cagcctttat gcagcgttta cgcgctctgt gtgacgagca cgggatcatg
     4861 ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt tgcgatggag
     4921 cagatgggcg ttgcgccgga tcttaccacc tttgcgaaat cgatcgcggg cggcttcccg
     4981 ctggcgggcg tcaccgggcg cgcggaagtg atggatgccg tcgctccagg cggtctgggc
     5041 ggcacctatg cgggtaaccc gattgcctgc gtggctgcgc tggaagtgtt gaaggtgttt
     5101 gagcaggaaa atctgctgca aaaagccaac gatctggggc agaagttgaa agacggattg
     5161 ctggcgatag ccgaaaaaca cccggagatc ggcgacgtac gcgggctggg ggcgatgatc
     5221 gccattgagc tgtttgaaga cggcgatcac aacaagccgg acgccaaact caccgccgag
     5281 atcgtggctc gcgcccgcga taaaggcctg attcttctct cctgcggccc gtattacaac
     5341 gtgctgcgca tccttgtacc gctcaccatt gaagacgctc agatccgtca gggtctggag
     5401 atcatcagcc agtgttttga tgaggcgaag cagtagcgcc gctcctatgc cggaggcgac
     5461 gctgcgcgtc ttgtccggcc tacggggatc caggtcggat aaggcgttta cgccgcatcc
     5521 gacaatctgt acgtgaacag gaagaaatct atgttggccg ggtaaggcgg agccgctctc
     5581 cggcaaaaag aatcaataac aattatacgc gtgacccgac gcgggaaatg tcggggcgct
     5641 ctcccaagtg acacactttc gagaggattc aggatggggc aatcatcgca accacatgag
     5701 ttaggcggcg ggctgaagtc acgccacgtc accatgttgt ctattgccgg tgttatcggc
     5761 gcaagtctgt ttgttggttc cagcgtcgcc atcgccgaag cgggtccggc ggtattactg
     5821 gcctatctgt tcgccgggct actggtggtt atgattatgc ggatgttggc ggaaatggcg
     5881 gttgccacgc ccgataccgg ttcgttttcc acctatgccg ataaagctat tggtcgttgg
     5941 gcgggttata ccatcggctg gttgtactgg tggttttggg tactggttat cccgctggaa
     6001 gctaacatcg ccgccatgat cctgcactca tgggttccag gcattcccat ctggttattt
     6061 tccctcgtca ttaccctcgc cttaactggc agtaacttat taagcgttaa aaactacggc
     6121 gaatttgagt tctggctggc gctgtgcaaa gtcatcgcta tcctggcctt tattttcctt
     6181 ggtgcagtcg caattagcgg tttttaccct tatgccgaag tgagcgggat ctcaagattg
     6241 tgggatagcg gcggctttat gcccaacggt ttcggtgcgg tattaagcgc gatgttgatc
     6301 accatgttct cgtttatggg cgcagaaatt gtcaccattg ccgccgcgga atccgacacg
     6361 ccggaaaaac atattgtccg cgccaccaac tcggttatct ggcgtatttc tatcttctat
     6421 ttatgttcta tttttgtcgt cgtggcatta attccgtgga atatgcccgg actaaaagcc
     6481 gtcggttctt atcgctcggt actggaattg ctcaatattc cccatgcgaa attgatcatg
     6541 gactgcgtga tattactttc cgtaaccagt tgcctgaact cggcgctgta taccgcttcc
     6601 aggatgctct actccttaag ccgtcgcgga gatgcgcctg cggtaatggg taaaatcaac
     6661 cgcagtaaaa ccccgtacgt ggcggtgtta ctctccaccg gcgcggcatt cttaacggtg
     6721 gtggtgaact attacgcgcc tgcgaaggta tttaaatttc tgatcgacag ctccggcgct
     6781 atcgccctgc tggtttattt agtcatcgcc gtttcacagt tgcggatgcg caaaattctg
     6841 cgagcagaag gaagcgaaat tcgcttgcgc atgtggcttt atccgtggct cacctggctg
     6901 gtcatcggct ttattacctt tgtgttggta gtgatgctat tccgtccggc gcaacagtta
     6961 gaagtgatct ccaccggctt attagcgata gggattatct gtaccgtgcc aattatggcg
     7021 cgctggaaaa agctggtatt gtggcaaaaa acacccgttc ataatacgcg ctgatcatga
     7081 tcaggagtca caccatgacc attacgtctc tggatggcta tcgctggctg aagaacgata
     7141 ttattcgcgg taattttcaa ccggatgaaa aattacgaat gagtttgctg acatcgcgtt
     7201 atgcacttgg cgttgggccg ttacgggaag ctctttcgca actggtggcg gaacggctgg
     7261 tcacggtggt gaatcaaaaa gggtatcggg tggcgtctat gtcagagcag gagctgctcg
     7321 atattttcga cgcccgcgcc aatatggaag cgatgttagt gagtctggcg attgcccgcg
     7381 gtggcgatga gtgggaggca gacgttctcg caaaagcgca tctgctgagt aagcttgagg
     7441 cctgtgacgc cagcgagaaa atgcttgatg agtgggatct gcgtcatcag gcgtttcata
     7501 cggcaattgt ggcgggctgt ggttctcact atttgctgca aatgcgtgaa cggttgtttg
     7561 atctggcggc gcgttatcga tttatctggc tgcggcgaac ggtgctttcg gtggaaatgc
     7621 tggaggataa acacgatcag caccagaccc tgactgcggc ggtactggcg cgagataccg
     7681 cgcgcgccag tgagttaatg cgccagcatt tactgacgcc aattcccatt atccagcagg
     7741 cgatggctgg caattaacaa ctcttccgga atacgcaaca cttgccccgg ataaatttta
     7801 tccgggcttt ttagcatcgg tttattcgct tcgaagattt tattgtacag attagcgtta
     7861 ccgtagacct gtttggaaat ggcactcaga gtgtcgccag acttaacggt ataaaactgg
     7921 ctggcagtgg ctggtgtcgc cgttttcacc tgatcatcga cactggcaat accggaaata
     7981 ttccccaccg caacaaggat tttctccttc gcctcctgac tcaggccgtc accagtgacc
     8041 gtcgctttgc cgtcggcaat ttgaatattc actttatcgg catccggtat accggttttg
     8101 ttcagatgct cctgcacctt cttcgcctga tcgtctttat cgtgctgacc tgtaaccgcg
     8161 tcccagagtt tttctccggc atctttcaca aaattgaaca gacccatagc tacctcattc
     8221 gttaacggaa acactcagaa agtgtagcag aggaacgcgg cgcgttaacg ccgcggggat
     8281 gagcacaggt ttaatcgcgg gtttgcaccc agaacgcgtg aatcaaaccg gggatatacc
     8341 caagcagtgt caacagaata ttaataatga acgcccaacc gaacccttta ccgagcagca
     8401 cgccgagcgg cggcagaatg atggtgatga cgattctcca gaaacccata tgtactccct
     8461 ataagaaaat tactcattgt ttaaaaagag attttatctc ttaagcgtag taattttagc
     8521 ggagcctgcc agtttcagct tacgctttta ctctcattta cgctgctgag gctggtatat
     8581 gttgcatttt tagtttagag tttacttaat ttagaaaaca cttaaataaa tatgactgaa
     8641 ctcgcgcaat tacaggccag tgccgaacag gcagcggcct tattgaaagc aatgagccac
     8701 cctaaacgat tgctgattct gtgcatgctt agcggttccc ccggcaccag cgcgggagag
     8761 ctgacacgca ttaccggact gagtgcctct gcgacatcac agcatctcgc tcgtatgcgg
     8821 gacgaagggc ttatcgacag ccaacgggat gcccaacgca ttctatattc cattaaaaat
     8881 gaggcggtaa atgccattat cgccaccctg aaaaatgtct attgtccgta aggagtcact
     8941 atggctttga caaccatttc gccgcatgat gcacaagaat taatcgcacg cggcgcaaag
     9001 ttaatcgata ttcgtgatgc tgatgaatat cttcgtgaac atattcctga agcagatctg
     9061 gctccattat ccgtgctgga acagtcaggt cttccggcta aattacgtca cgagcaaatt
     9121 atattccact gccaggcagg taaacgcacc agtaataatg ccgataaatt agcagcgatt
     9181 gccgcccccg cagaaatatt tttactcgaa gatgggattg atggctggaa aagagctgga
     9241 ttgccagtag cggtaaataa atctcaaccc tttccgttaa tgcgccaggt gcagatcgct
     9301 gcgggtggtt taatattaat cggcgttgta ctgggttata ccgtaaatag cggtttcttc
     9361 ttattaagtg gctttgttgg tgccgggtta ctgtttgcag gaatcagcgg tttttgtgga
     9421 atggcaaggt tgttagataa gatgccgtgg aaccaacgag cttgagaagc gacgccggac
     9481 gcgccctagc agcgacatcc ggcctcagta attagatcag gaaatcgtcg agagatttac
     9541 cttctgccag cgcctgagca attggcttcg gtgtacggcc ctgaccggtc caggttttag
     9601 tttcaccgtt aacatcggtg aatttatatt tcgccggacg cggctggcgt tttttaccag
     9661 cgcgtggtgc agcagcagag ctattaccta ataactcttc cgggttaatt ccgtcagcct
     9721 tcatcagctc cagccaggtg ctaatttttt cctgacgctc agccagttca cgctgctgct
     9781 gttcttcttc ttcacgtctt tctttagtga caaccctgaa tttttcgagc atttcttcaa
     9841 gaacgtcaat ggagaattcg cgagccatcg cacggagagt gcgaatgtta tttaaacttt
     9901 gtaacattac ggacataata aagaaaacct tttaacgcca aaacaaaaaa gtatttcgtc
     9961 gcgtagtgta agcgatcact tattatattt c
//
LOCUS       DAJOWB010000150         9956 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJOWB010000150 DAJOWB010000000
VERSION     DAJOWB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9956
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.150"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(1..304)
                     /locus_tag="OHO85_004146"
                     /pseudo
     CDS             complement(1..304)
                     /locus_tag="OHO85_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
     gene            407..685
                     /locus_tag="OHO85_004147"
     CDS             407..685
                     /locus_tag="OHO85_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FaeA/PapI family transcriptional regulator"
                     /protein_id="HCQ2673441.1"
                     /translation="MSEYMKNEILEFLNRHDGGKTAEIAEALAVTDYQARYYLLLLEK
                     AGMVQRSPLRRGMATYWLLKGEKQAGQSCSLCKLCRPVVIRSVTGLAR"
     gene            619..1275
                     /locus_tag="OHO85_004148"
                     /pseudo
     CDS             619..1275
                     /locus_tag="OHO85_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
     gene            complement(1451..1753)
                     /locus_tag="OHO85_004149"
     CDS             complement(1451..1753)
                     /locus_tag="OHO85_004149"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673442.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(1840..2655)
                     /gene="sul2"
                     /locus_tag="OHO85_004150"
     CDS             complement(1840..2655)
                     /gene="sul2"
                     /locus_tag="OHO85_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCQ2673443.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(2896..3462)
                     /locus_tag="OHO85_004151"
     CDS             complement(2896..3462)
                     /locus_tag="OHO85_004151"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal sequence protein"
                     /protein_id="HCQ2673444.1"
                     /translation="MLKRRDAFLKKSALAVSVALLLSAQAQAVLTGPVDANSSSLLIG
                     ENSFITNSTGTANNTFLLGGGAFNMDSPGSLQFGSFSGVYNSPHSVTLGRDAGQAESK
                     YGVAIGKSAEVLNSQQSVAIGGWAGIENSSGSVALGHGSQVSGENNVVSVGAGPEGYG
                     ESVKGAPETHSIVNKTFLFFIFNGLFSC"
     gene            complement(4286..4399)
                     /locus_tag="OHO85_004152"
     CDS             complement(4286..4399)
                     /locus_tag="OHO85_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2673445.1"
                     /translation="MVKLALQPGASVARIAREHDINDNLLFKWLRLWQNVR"
     gene            complement(4662..4838)
                     /locus_tag="OHO85_004153"
                     /pseudo
     CDS             complement(4662..4838)
                     /locus_tag="OHO85_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
     gene            4898..5589
                     /locus_tag="OHO85_004154"
                     /pseudo
     CDS             4898..5589
                     /locus_tag="OHO85_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            5992..6183
                     /locus_tag="OHO85_004155"
     CDS             5992..6183
                     /locus_tag="OHO85_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HCQ2673446.1"
                     /translation="MVTLTINRKPQGIFSKSPATPLQDKTDTAYKMKAGDQIPQLNDK
                     QKPADKTPWRHDKTPEQKP"
     gene            6252..6500
                     /locus_tag="OHO85_004156"
     CDS             6252..6500
                     /locus_tag="OHO85_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HCQ2673447.1"
                     /translation="MGIPDDLIQDIAIRELAFGAGTLHAAVASYVQSPRYYRALIAGG
                     ARYNLNGQPCGEVTPQEQKEAETRLMMLNDRRKARKPR"
     gene            complement(6519..7067)
                     /locus_tag="OHO85_004157"
     CDS             complement(6519..7067)
                     /locus_tag="OHO85_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673448.1"
                     /translation="MQITKALISEPGDIRRFVQQAVDHWPNLLAFHFTLYSAEGNING
                     QQIHAFCTSFYRQVHERITERNHTASPSSPVVLRWLREQHGGATIRCLLLFSQELFCH
                     PRASVTVDEECSQLVDLLQQTWQVISAGGQCRVEKRFQVVRGDTSGQYVALKTVALSL
                     GLPVVIAITHRPVQRCTLITAQ"
     gene            complement(7333..7569)
                     /locus_tag="OHO85_004158"
     CDS             complement(7333..7569)
                     /locus_tag="OHO85_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCQ2673449.1"
                     /translation="MLTSMTGHDSVLLRADDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQHRIEASRGAAA"
     gene            complement(7638..8210)
                     /locus_tag="OHO85_004159"
     CDS             complement(7638..8210)
                     /locus_tag="OHO85_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673450.1"
                     /translation="MNAIPYFDYSLAPFWPSYQNKIIGVLNRVLREQSCSRIRRILLR
                     LPGEHGNIFSDRKTWFGMAFIETVSALMNATPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFPSPGEAAPYQKQITRDVVLDSCSPDCEERLKELIWAF
                     SDFARDRRGVRDPEARYLAGNPWYPVAGQL"
     gene            complement(8468..9034)
                     /locus_tag="OHO85_004160"
     CDS             complement(8468..9034)
                     /locus_tag="OHO85_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCQ2673451.1"
                     /translation="MPKSYTPNWFFTALLDNHINQMMARYSCLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENMVGFFWVIEWTVDHGFHAHVVFWLDRQRVKKIY
                     PFAERITECWRSITHNSGSAHRCTYQPHYTYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCNEVPERPAAGRPRKPHF"
BASE COUNT         2701 a         2433 c         2444 g         2378 t
ORIGIN      
        1 acgtcactgc ccggctgtat gcgcgaggtt accgactgcg gcctgagttt tttaagtgac
       61 gtaaaatcgt gttgaggcca acgcccataa tgcgggcggt tgcccggcat ccaacgccat
      121 tcatggccat atcaatgatt ttctggtgcg taccgggttg agaagcggtg taagtgaact
      181 gcagttgcca tgttttacgg cagtgagagc agagatagcg ctgatgtccg gcagtgcttt
      241 tgccgttacg caccaccccg tcagtagctg aacaggaggg acagctgata gaaacagaag
      301 ccactggagc acctcaaaaa caccatcata cactaaatca gtaagttggc agcatcacca
      361 cagaaagcat gatttttatt attcgaagaa actttgttac agtgagatga gtgaatatat
      421 gaagaatgaa atactggaat ttctgaacag gcatgatgga gggaaaacgg cagaaattgc
      481 ggaggcgctg gcggtaacgg attaccaggc ccgctattac ctgttattac tggaaaaagc
      541 aggtatggtt cagcgctcac cattaagacg gggcatggca acatactggc ttctgaaagg
      601 agagaagcag gcggggcaga gttgttcact ttgcaaactc tgccgaccag tggtgatccg
      661 ttcggtgacg ggattggcaa ggtagccggg tccagcctgc acgccggcgt ggcggccagg
      721 gccgatgaac gcaagaagct cgaacggctg tgccggtaca tcagccgccc ggcggtatcc
      781 gagaagcggc tgtcgttaac acgaggcggc aacgtgcgct accagctcaa gacgccgtac
      841 cgggacggca ccacgcacgt cattttcgaa ccattggatt tcattgcaag gctggccgcc
      901 ctggtaccga agcccagagt caacctaacc cgcttccacg gggtgttcgc acccaacagt
      961 cggcaccggg cgttggtcac gccggcaaaa cggggcaggg gcaacaaggt cagggtggct
     1021 gatgaaccgg caacaccagc acaacggcga gcgtcgatga catgggcgca acggctcaag
     1081 cgtgttttca atatcgacat cgagacctgc agcggctgcg gcggcgccat gaaagtcatc
     1141 gcctgcattg aagaccctat agtgatcaag cagatccttg atcacctgaa gcacaaagcc
     1201 gaaaccagcg ggaccagggc gttacccgaa agccgggcgc caccggctga gctgctcctg
     1261 ggtctgtttg actgacgagc ctgaaggcca acgataccaa tcaaaatgct gcgttcacag
     1321 cgccgcggca gggatccgcc gtgctggttg tcggaaaagg agccgctagt gggaaagagg
     1381 agggtaaatt ttcagcgttg ctggctcccc gtcagccgga ttgggttgca tcgcaggggt
     1441 gtcgaaagag tcaactgcgg tccaaagctg ttggacttgg gtgaaaaggg cgtttattct
     1501 tcctatacgt tgtcggcagc gggccaaaaa ggaatacgtc catgcccatc gaggtgaaac
     1561 cggctgtgag cgcgggttca agcatatagc ccgacaggcg cgtatccttg ccgatcacga
     1621 cacgatggcg gtggtcaccg cgacgaaaga cacggccagc cgccatgccg acgcgcaagg
     1681 cggtttccgc cgtcatcgcg ccttcgttgg ctttgccacg aataccgtct gtgccgaaat
     1741 atttgcgcac cataaggtcg attatcctgt cgtcgggtcg ccctcaaagg ggacatgcct
     1801 gctgaaccgc gaatatagag aaatatcccg aatgtgcagt taacgaattc ttgcggtttc
     1861 tttcagcgcc gccaataccg ccagcccgtc gcgcaagggg cgcggctcgt gtgtgcggat
     1921 gaagtcagct ccacctgcgg cggcggcaag ctctgcagcg agtgtcgcgg ccccgacatc
     1981 ccccggacca cggcctgtga gcgcgcgcag aaaggatttg cgcgaaacag acagaagcac
     2041 cggcaaatcg aagcgcagcc gcaattcatc gaaccgcgcc agcaccgaga gcgaggtttc
     2101 gggagcagcc cccagaaaaa accccatgcc gggatcaagg acaaggcggt tgcgtttgat
     2161 accggctccc gtcagcgccg cgatgcgcgc gtcaaagaac gccgcaatgt gatccatgat
     2221 gtcgccagcg ggtgcctcgc gccgatctgc ctgcccgtct tgcaccgaat gcataacgac
     2281 gagtttggca gatgatttcg ccaattgcgg atagaacgca gcgtctggaa aaccgcgaat
     2341 atcattgaga taggccacac cacgcgacaa ggcataggct tgcgtcgcgg gttgataact
     2401 gtcgagcgag acgggaatgc catctgcctt gagcgcgtcc agcaccggcg cgatacgcgc
     2461 gatttctgtg tcggacgaaa caggcgcggc gtcgggattg ctggatgccg gaccgaggtc
     2521 gatcacatct gccccctcgg ccatcagctt acgcgcctgc gcaatggctg cgtctggcgc
     2581 cagataccgg cctccatcgg agaaactgtc cgaggttatg ttgacgatgc cgaaaatgat
     2641 gagcgattta ttcatggggg cttctataat aataataatc gagcatgagt ctcatacgga
     2701 tgctcgggtc gaaagggaat ccccaggcga gtaacctgtt tgcggtgatc cattagctgc
     2761 aggagcagaa tagcatacat ctggaagcaa agccaggaaa gcggcctatg gagctgtgcg
     2821 gcagcgctca gtaggcaatt tttcaaaata ttgttaagcc ttttctgagc atggtatttt
     2881 tcatggtatt accaattagc aggaaaataa gccattgaat ataaaaaata aaaatgtctt
     2941 gtttacaata gagtgggttt ccggtgctcc tttgacactc tctccatatc cctcaggccc
     3001 ggcaccaaca gataccacgt tattttctcc cgacacctgt gagccatggc ccagagctac
     3061 agagcctgaa gaattttcta ttccagccca tcccccaata gcaacactct gctgtgaatt
     3121 aagtacctca gcgctcttac ctatggcaac accatatttt gactcagcct gaccggcatc
     3181 ccttcccagg gtgacactgt gtggagaatt atacactccc gaaaaagagc caaactgaag
     3241 acttcctggt gaatccatgt taaaagcgcc acctcccaaa aggaaagtgt tattagctgt
     3301 accagttgaa ttcgtaataa aagagttttc tccaatcagt aagctggaag aattagcatc
     3361 aacaggccct gtcaaaacag cctgagcctg agccgagagc agcagggcca cactcactgc
     3421 cagcgcgctt ttctttaaaa aagcatcacg tcttttaagc attctgttat tctcctgtat
     3481 tattacttca ccagccagaa cggtagtgcg gagcaaaaat acagtgataa gaatcctctt
     3541 ttattaattt aataatgcca ggaatattat tgtgtataat atgcgacctc aatttaagca
     3601 gcagaaaacg actggcatta aaatcattaa attataaaca tatacaactt tgtaaaggtg
     3661 gttataataa acctgattac tttaacgtct gtatacctgc actgctgata tcacaaaaca
     3721 ataaaaatcc tggttaatat ctctgatgat tgaatgagaa attcacgatg tctggtgaga
     3781 attctgttca aaacgaggaa tgcagacgta catgtggcag gcatggactt ctaccaattt
     3841 aacttttttc cttccttgta tttacacatt gcacattttg cgctacaaaa atttcacgtg
     3901 gcacgcacca cgtgatccgt agaaaagcct cttgttacga atttcgtaaa ccgcacgtct
     3961 gtctggtcgt gcggattttt tatccgtcaa cttcacatga aacccgccag agcactgttt
     4021 ccggattcag acatcaggca tattatcatc cacgatgtcg tgaacaagat cacacttttg
     4081 caattccttt attgatcgaa agcagttaat cacggaaatt aattaccgga gtgaaagcat
     4141 tactgcatcg taaatatcta ccatttttga ctacaccctc tgacagaatt ttcagagggt
     4201 ttttttatca gtttcaccat ccaaccctgt acgccagaat ggtttcactg tcactcagtt
     4261 ctgctattgc ctttttcatc tccctttacc ttacgttctg ccagagtctg agccatttga
     4321 acaggaggtt atcattgata tcgtgttccc gggcaatacg ggcaacagag gctcctggtt
     4381 gcaaagccag tttaaccatt tgaagtttaa ctcatttgaa aatgatctgc ggggttctgc
     4441 ggataatgtt tttgctccat aacaggcgcc caccagtttg aaaaggtggg cacctacgtt
     4501 accaatactg gcttaatgga tacatacggc ggtcggttct tacggtaaaa cgacacaacc
     4561 aaacgcttct tatcctgcaa tgaactgctt tattaccgag tttagtgacc gcacagacac
     4621 tcacttttaa gaaaaacaga atggtaaaca aactccatga attacaatag ctctctggct
     4681 gccgccacaa tagcttgcgc ggttaacccg tactctctct gtaaaaagtc ctgagtttca
     4741 acctggccat accgctcttt aacccccacc cgacgcatgg gcacaggaca agtttctaca
     4801 agcacttcag caactgctga acctaatcca ttatgaatac ggtcatgact ccaattagtt
     4861 gaattagggg tataatagcc acttttaagg ttctttcatg gtcacagtta accttcattg
     4921 ctcctgttgc cagtccgttc aggtttaccg tcacgggaag aacccaaagg acatgatcga
     4981 tttcgctgcc gtgactgtca tcgagtgctt cagctcactt accgttacga ggcccgtaaa
     5041 ccgggagtta aagagcaaat cactgaaatg gctttcaacg gtgccggagt tcgcgatact
     5101 gcccgtacgc tgaaaatcgg cattaatact gtcatccgga ctttaaaaaa ctctcgccac
     5161 gaagaataac gtcatacccg gtcgctcatg ctgatatggc tctcatctgc gaacttgatg
     5221 agcaatggag ttttgtcggc agtaaagcgc gacagcactg gctctggtac acgtacaaca
     5281 cgaaaactgg tggcgttctg gcctacactt ttggcctacg gactgattaa acctgtcgtg
     5341 aattgctggc gctgcttgcc cttttaccat cggtatgttg accactgacg aatggggtag
     5401 ttacaccaga gagttaccga aggagaagca tctgactggt aagattttta ctcagcgcat
     5461 tgagcgcaac atctacctct gagaacccga atcaaacgtc tggcccgcag aactatctgt
     5521 ttctcgcgct ctgtagaact tcatgaaaaa gtcatcggtg cctttatcga aaaatatatg
     5581 ttctactaat tggagtcacc accttcgttt gtttaaagaa cctgctggtc ctgccattaa
     5641 tattacctgc tgattgcttc acatccggat tcctcgcact gcgcgcgggt tattgtttcg
     5701 ttttcggcca cggggtcgcc agggctctgt ttgcaggcca tgatggcctg acctgtactg
     5761 tttctcaggc actgagatgt ccgtttacgc catttgttcc cggatatcct taacctctga
     5821 cgtttgttcc ctgcccctct gtctgataac gaatacgctc acaacgcaca gtactgaaca
     5881 ataccgacaa caacatgtct tacatgaaat tcgctgtaac atccaccgga aaacattttc
     5941 agatatcgga tgaggtacta cttccattga cagcgcgagg taacaaaatt gatggtaact
     6001 ctgaccatta accgtaaacc gcagggtatt ttcagtaaat caccggcaac gccgctgcag
     6061 gataaaacgg acacagcata caaaatgaag gccggtgacc agataccaca gttgaacgat
     6121 aaacagaaac ctgctgataa aacaccgtgg cgacatgaca aaacgccaga gcaaaaaccg
     6181 taagcgcatt attcgtcttg tggaactctg gcctgagtca ttcaaccggg aaaaaaacaa
     6241 aaccacttaa agtggggata cctgacgacc tgatacagga tatcgccatc cgagaactgg
     6301 cgtttggcgc tggtacatta catgcagcag tggcgtctta tgtgcagtct ccgcgctatt
     6361 accgtgcgtt aatagcaggt ggcgcccgtt acaacctgaa cggtcagccg tgcggggaag
     6421 tgacaccaca ggaacagaaa gaggcagaga cccgtttaat gatgctgaat gaccgacgaa
     6481 aagccagaaa acctcgttga ctgcacaact gcgctgattc actgagctgt aatcaatgta
     6541 cagcgctgta ccggacgatg ggtaatggcg atcacaaccg gtaaccccag agacaatgcg
     6601 actgttttta acgcaacata ctggccagat gtatcacccc ggacgacctg aaaacgcttt
     6661 tccacccggc attgtccgcc agcgcttatc acctgccaag tctgttgtag taaatccacc
     6721 agttgtgaac attcttcatc aactgtcaca ctggctcgcg gatggcagaa aagctcctgg
     6781 ctgaacagca acaggcatcg tattgttgct cctccatgtt gttcccgcaa ccagcgtaat
     6841 accaccggcg aggatgggct ggccgtgtga ttacgctcag taatacgttc gtgcacctgt
     6901 cggtaaaagg aagtacagaa tgcatgaatc tgttgcccgt tgatgtttcc ttctgccgaa
     6961 tagagcgtga agtgaaatgc caacagattt ggccagtggt ccaccgcctg ctgaacaaaa
     7021 cgccggatgt ctcccggctc tgaaattaag gctttggtta tttgcacgaa atgttccttt
     7081 tcaaaccctg ttgaattttg ttaagccagt gcagacacgc aggaaagcac gccagcaccg
     7141 gtgtaatgcc agttataatc aataagaatg gaatgacgaa tcagtcagga agagctacaa
     7201 cctgctggct gactggagaa tgtctgcaag aagatgaacg taaatgtcgc cagcaccagg
     7261 gacagggctg atttccacaa taccaacgct ggtattttgc acattgccag cgtaccggca
     7321 ttacaacacg tttcatgctg ctgcccctcg tgatgcctcg attcgatgct gcatccactg
     7381 ctccacttca cttttgtacc agcggctgct gcgccccagt ttgatgggtt tagggaaatg
     7441 gccttcactg atcagcttgt aaaaccattt gtcggtcata ccagtgaagc tggtgatgta
     7501 gttcatgtcg atcaggggat cgtcggcacg cagcaatacg ctgtcgtgac ctgtcattga
     7561 ggtcaacatg gtcagaatgt cctctgtgag atttatgaat gttgggcaaa tgcgggtgaa
     7621 tctgctgagc tgagctttca gagctggcct gctacgggat accagggatt accggcgagg
     7681 taacgggctt ccggatcgcg cacaccacga cgatcgcggg cgaaatcact gaacgcccat
     7741 atcagctctt tgagtctctc ttcgcagtcc ggtgaacaac tgtccagcac cacatcccgg
     7801 gtgatctgct tctggtacgg tgctgcttca cccggtgatg ggaagcccag tacgttgctc
     7861 caggcatcca gaatcagccg atccagttca gggccgtcga aatactcctg tctgacgcac
     7921 agcaccacgt ggtattccgg tttttcagga tgacgtgtca ggagccagca gaggtcacgt
     7981 ccgggtgttg cattcatcag cgcactgacg gtttcgataa aggccatacc gaaccaggtt
     8041 tttctgtcgc tgaagatgtt gccatgttcc cccggcagac gaagcaggat ccgccgtatt
     8101 cgggagcagg actgctcgcg caacacacgg ttgagaacgc cgatgatttt gttctgataa
     8161 gatggccaga agggggccag gctatagtcg aaataaggga tagcattcat aagatatgct
     8221 ccgttatgcg cagcccacaa tgcgtcctgc ccgtgaataa gctgacgtgt tatggtttgc
     8281 ggatggggtt gatggaataa gtgaagaacc ggggcagaac gggaagatca ggtcaccgac
     8341 aagggacaga acaccggatg tggttcagag tgaatgatct ccgttgactt cagttcacat
     8401 cattaagcaa ggcggtaagg ttttaccgac ggaatgtttc caggacagga agctcaggat
     8461 ttaagcttca gaagtgaggc ttacgaggac gccctgcagc aggacgttca ggaacttcat
     8521 tgcagccgta ggcacacagc ccgtcttttt gctcttcttt tgccagataa tgcagggcac
     8581 cgcgaatatt atcgatgctt tcaggatcgt tgtggcgcac aggaatgttg atgttgtatg
     8641 tataatgcgg ctgatatgta cagcggtgtg ccgaaccgct gttatgcgta atagaccgcc
     8701 agcattccgt aatccgctcc gcaaagggat atattttttt aaccctctga cgatcgagcc
     8761 agaagaccac atgcgcatga aaaccatgat caaccgtcca ttcaatcacc cagaagaagc
     8821 caaccatatt ttcaaactgt tcaacctgct ccagcagcat acgcagttgt aattcaagcc
     8881 aacgatgatc aggttgtaag aaatcaggcg tatccttcct gtagaagaaa tccatccgta
     8941 aggcccgcag gcaggaatag cgtgccatca tttgattgat gtgattgtca agtaaagcgg
     9001 taaaaaacca gtttggtgtg taagatttag gcatgttgat gactcctgaa agttaatgat
     9061 gttttaagct ctgtgagcga attaatgctt tcgctcataa tcagatctaa aacggtaata
     9121 acttacagat ggtatttatc aggcatgctg taacagaatg gatacgacgg attattaacg
     9181 acacacagcg ttgtgctgaa gggtatgagt tatctgatgg cattgtctat agagatgaga
     9241 taaacgaggt gcgaagaaaa tacagattca atacaacaca gtgaaataca gaaaaatacg
     9301 aactgtaatc ccgctgccag gcggttagga aacatattac aactataagc tcatgatttg
     9361 taaatttgct ctcagtgagt ctgaaaaaac gtttataagg tcaccacaat aaactcacac
     9421 gcgcttattc aatctgttct ccaggatatg aacgttaacg acacacgata gcataagaca
     9481 acatacaatg gatgaataat tttcacccag taccttaaac aatacaaccg gcaacaaata
     9541 taatcagaaa ttcactctgt taattctgtt gtttatcctg cgctgttaaa catgacggaa
     9601 ccatggatac cttatcaaag agtaaacatt cagttaatct ttgatgacag agaaattgtt
     9661 tccaaccaga atagttaaaa cacttcagat aaacaatcat tgttaagcaa atacagttca
     9721 tgatggataa attaaaatca attactgttt cactgaaaat gagaacagat ggatatcaac
     9781 aatagcgtag ctgtagagaa taacttatct gagtaacgta tgaattacag taattatgat
     9841 tgaatgaggg aagaagtgga tattatgata atggtgacaa ggaagattag agaaggacat
     9901 gaatataatt ggatgaggat aagagtaatg gaatgtgtaa agatagagtg aatata
//
LOCUS       DAJOWB010000151         9862 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJOWB010000151 DAJOWB010000000
VERSION     DAJOWB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.151"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            30..1412
                     /locus_tag="OHO85_004161"
     CDS             30..1412
                     /locus_tag="OHO85_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCQ2673452.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATPPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            1449..2171
                     /gene="folM"
                     /locus_tag="OHO85_004162"
     CDS             1449..2171
                     /gene="folM"
                     /locus_tag="OHO85_004162"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCQ2673453.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(2168..2503)
                     /locus_tag="OHO85_004163"
     CDS             complement(2168..2503)
                     /locus_tag="OHO85_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCQ2673454.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            2632..3351
                     /gene="rstA"
                     /locus_tag="OHO85_004164"
     CDS             2632..3351
                     /gene="rstA"
                     /locus_tag="OHO85_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCQ2673455.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            3355..4656
                     /gene="rstB"
                     /locus_tag="OHO85_004165"
     CDS             3355..4656
                     /gene="rstB"
                     /locus_tag="OHO85_004165"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCQ2673456.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IIHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            4732..5661
                     /gene="tus"
                     /locus_tag="OHO85_004166"
     CDS             4732..5661
                     /gene="tus"
                     /locus_tag="OHO85_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCQ2673457.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(5658..7061)
                     /gene="fumC"
                     /locus_tag="OHO85_004167"
     CDS             complement(5658..7061)
                     /gene="fumC"
                     /locus_tag="OHO85_004167"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCQ2673458.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(7204..8850)
                     /gene="fumB"
                     /locus_tag="OHO85_004168"
     CDS             complement(7204..8850)
                     /gene="fumB"
                     /locus_tag="OHO85_004168"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCQ2673459.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            9049..>9862
                     /gene="manA"
                     /locus_tag="OHO85_004169"
     CDS             9049..>9862
                     /gene="manA"
                     /locus_tag="OHO85_004169"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCQ2673460.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDN"
BASE COUNT         2282 a         2550 c         2461 g         2569 t
ORIGIN      
        1 tcttatcacg ttttaatcac tggatatcga tggaaaagaa actgggactg agcgcactca
       61 ccgcgctggt attaagctca atgctgggcg cgggtgtttt cagtctgccg caaaatatgg
      121 cggcagttgc cagcccggca gcactgctca tcggctgggg tattactggc gctggcattt
      181 tattgctggc ctttgccatg ctgatcctca cgcgcattcg tcccgaactt gacggcggta
      241 tctttaccta tgcccgcgaa gggtttggcg agctaatcgg cttttgttcc gcatggggat
      301 actggctgtg cgcagtcatc gccaacgtct cctatctggt aatcgttttt tccgcgttaa
      361 gcttttttac ggacacgccg gaattgcgcc tgttcggcga tggtaatacc tggcaatcga
      421 tagtcggtgc atcggcttta ttgtggattg ttcacttttt gatcctgcgc ggggtgcaaa
      481 ccgctgccag cattaacctg gtggcgacac tggcaaaatt gttgccgctg ggtctgtttg
      541 ttgtgctggc gatgatgatg ttcaaactgg ataccttcaa gctcgacttc accggacttg
      601 cacttggcgt acccgtgtgg gaacaggtga aaaacaccat gctgatcacc ctgtgggttt
      661 tcattggtgt ggaaggtgcc gtggttgttt ctgcgcgtgc gcgtaataaa cgtgatgttg
      721 gcaaagcgac actgctggcg gttctctccg ctctgggcgt ttacttgctg gtaacgctgc
      781 tttcgctggg cgtggtggca cgtcctgaac tggctgaaat tcgtaacccg tcaatggccg
      841 gtctgatggt ggaaatgatg ggaccatggg gcgaaatcat catcgctgcc ggtttgatcg
      901 tttccgtttg cggcgcgtac ttaagctgga ccatcatggc ggcagaagtt ccgttcctgg
      961 ctgccactca taaagcattc ccacgcattt ttgcgcgcca gaacgcacaa gctgcgccat
     1021 ccgcctcgct gtggttgacc aacatctgcg tgcaaatttg tctggtactc atctggctga
     1081 ccggttccga ttacaacacg ttgctgacca tcgcctcaga aatgattctg gtgccctatt
     1141 tcctggtcgg cgcgttcttg ctgaaaatcg caacccctcc actgcataaa gcggtaggtg
     1201 tcggtgcctg catttatggc ttatggttat tgtatgcctc tggtccaatg cacttgttgt
     1261 tatccgttgt tctgtatgcg ccggggctgc tggttttcct gtatgcgcgt aaaacgcata
     1321 cacatgataa cgtactgaac cgccaggaaa tggtgctaat tggcatgctg ttaattgcct
     1381 cagttccggc gacctggatg ctggtgggat aacgttcatc ccatcgttgg ttagcaaagg
     1441 agataacgat gggtaaagcc cagcccttgc caatattaat tactggcggt ggtcgtcgca
     1501 tcggcctcgc cctcgcatgg catttcatta atcaaaagca accggtgatt gtcagctatc
     1561 ggacacacta tccagccatt gatggactga ttaatgcagg tgcgcagtgt attcaggctg
     1621 atttttcgac caacgacggt gtgatggcgt ttgccgatga agtactaaaa agcacccatg
     1681 gtctgcgtgc tattttgcat aacgccagtg cgtggatggc ggaaaaaccg ggtgcgccac
     1741 tggccgacgt actggcttgc atgatgcaga tccacgttaa taccccatac ctgctcaacc
     1801 atgcgctgga aagattactg cgtgggcacg gacacgccgc cagcgatatc attcacttta
     1861 ccgattatgt ggtggagcgc ggtagcgaca aacatgttgc gtatgctgca agcaaagcgg
     1921 cactggataa tatgacccgc tcgtttgccc gcaagctggc accggaagtg aaagtgaatt
     1981 ctattgcgcc atcgctgatc ctgtttaatg aacatgatga tgccgaatat cgacaacagg
     2041 cgctgaataa atcactgatg aaaaccgcgc ctggcgagaa agaagtgatc gacctggtcg
     2101 attacttact taccagttgc tttgtcaccg gacgcagttt cccacttgat ggcggtcgtc
     2161 atctgcgtta atgcagcttt atccagcata taatcaacac ccatgcgctt atcccccaac
     2221 acgcaacaga accaacaaaa gcggcgggca agcgcatcat tccggtgaaa taccacagcg
     2281 acaccaggta aacaaaatag ggaataatcg accacatact aaaaatgatg gttgcgcgta
     2341 aggcttcaat gccgcgttcg ctggcaacaa tataatgcgc gataagcgca aaggtcggga
     2401 aaagtggaat cagcccggcg atataataat ttttcgtttt tgctaaaaca ccaatcaaca
     2461 gcactaccag cgcaccgagc gcggctttga ttaccagccc cattttttta ccttaacact
     2521 tccataacaa gtcatcagta gaatacctga tgaaaacttg tttagaaacg attgatagta
     2581 agtaaaaaca gcgcggtgta ttgtgacgtt tttatatcta ccgtgaataa tatgaacact
     2641 atcgtatttg tggaagatga tgcggaagtc ggttcactga ttgccgcgta cctggcaaaa
     2701 catgatatgc aggttaccgt agagccgcgc ggcgaccagg ccgaagaaac cattttgcga
     2761 gaaaatccgg atttggtgtt actcgacatc atgctaccag gcaaggacgg catgaccatt
     2821 tgtcgtgatt tacgcgcaaa gtggtctgga ccgattgttc ttctaacctc tctcgatagc
     2881 gatatgaacc acatcctggc actggaaatg ggtgcctgcg actatattct caaaacgacg
     2941 ccccctgctg ttttgctagc gcgtttacgt ttgcatttgc gtcagaatga gcaagccaca
     3001 ctgaccaaag gtcttcagga aacgtctctg actccctaca aagccctgca tttcggcacg
     3061 ttgaccatcg atcccatcaa ccgcgtagtc accctggcta acactgaaat ctcgctctcg
     3121 acagctgatt tcgaattatt gtgggaatta gctacccatg ccgggcaaat catggaccgc
     3181 gatgcattgc tgaaaaattt acgcggcgtc agttatgacg gactggatcg tagcgtggac
     3241 gtggctattt cgcggttaag aaaaaaactg ctcgataacg ccgcagaacc ttatcgcatt
     3301 aaaactgtgc gtaacaaagg ctatcttttt gcgcctcatg catgggaata agcgatgaaa
     3361 aaactgttta tccagtttta cctgttattg tttgtctgct tccttgtgat gtctctgctg
     3421 gttgggctgg tgtacaaatt taccgccgaa cgcgcgggca aacagtcgct ggatgatttg
     3481 atgaacagtt cgctgtatct gatgcgtagc gaattgcgtg agatcccccc acacgattgg
     3541 ggtaagacgc tgaaggagat ggatttaaat ctctctttcg atctgcgtgt cgagccactg
     3601 agtaaatacc atcttgatga tatttccatg caccgactgc gtggcggcga aattgtcgcc
     3661 ctggacgatc agtacacgtt tttgcagcgt atcccgcgca gccactacgt gctggcagtt
     3721 ggtcctgttc cttatcttta ttatctccat cagatgcgat tgctggatat cgccctgatc
     3781 gcttttattg ctatttccct cgcctttccg gtgtttatct ggatgcgtcc gcactggcag
     3841 gatatgttaa aactggaagc agcggcgcaa cgatttggcg atgggcatct caatgagcgt
     3901 atccactttg atgagggttc gagctttgaa cgacttggta tcgcatttaa ccagatggcg
     3961 gacaatatca acgccttgat tgccagcaaa aaacagctta ttgacggtat cgctcacgaa
     4021 ctgcgaacac cgttagtgcg cctgcgttat cgactggaga tgagcgataa cctgagcgcc
     4081 gccgaatccc aggcgttgaa tcgtgatatc agtcaacttg aagctttaat tgaagagctg
     4141 ctgacttatg cccgactcga tcgcccacaa aacgagcttc atcttagcga accagacctg
     4201 ccgttgtggc tgtcaacgca tctggcagat attcaggcag taacgcccga taaaacggta
     4261 cggataaaaa cgctcgtgca aggccattat gcggcgttgg atatgcgctt aatggagcgc
     4321 gtgctggata atttgctcaa taacgccctg cggtactgcc attcaacggt tgaaaccagc
     4381 ctgctactgt cggggaatag agcgacatta attgttgagg atgatggccc agggattgcc
     4441 ccagaaaacc gcgaacatat ctttgaacct tttgttcgcc tcgatcccag ccgggatcgc
     4501 tcaaccggcg gctgcgggct ggggctggca attatccact ctatagcact ggcaatgggc
     4561 ggtacggtta attgtgacac cagcgaactg ggtggtgctc gcttctcgtt tagctggccg
     4621 ttatggcata acatcccgca atttacctct gcctgacact acgcgcacga tggtcaagtc
     4681 accaccattg tgctataaat taaggatgtt gtaactaaag tggttaatat tatggcgcgt
     4741 tacgatctcg tagaccgact caacactacc tttcgccaga tggaacaaga gctggctgca
     4801 tttgccgctc atcttgagca acacaagcta ttggttgccc gcgtgttctc tttgccggag
     4861 gtaaaaaaag aggatgagca taatccgctt aatcgtattg aggtaaaaca acatctcggc
     4921 aacgacgcgc agtcgctggc gttgcgtcat ttccgccatt tatttattca acaacagtcc
     4981 gaaaatcgca gcagcaaggc cgctgtccgt ctgcctggcg tgttgtgtta ccaggtcgat
     5041 aacctttcgc aagcagcgtt ggtcagtcat attcagcaca tcaataaact caagaccacg
     5101 ttcgagcata tcgtcacggt tgaatcagaa ctccccaccg cggcacgttt tgaatgggtg
     5161 catcgtcatt tgccggggct gatcaccctt aatgcttacc gcacgctcac cgttctgcac
     5221 gaccccgcca ctttacgctt tggttgggct aataaacata tcattaagaa tttgcatcgt
     5281 gatgaagtcc tggcacagct ggaaaaaagc ctgaaatcac cacgcagtgt cgcaccgtgg
     5341 acgcgcgagg agtggcaaag aaaactggag cgagagtatc aggatatcgc tgccctgcca
     5401 cagaacgcga agttaaaaat caaacgtccg gtgaaggtgc agccgattgc ccgcgtctgg
     5461 tacaaaggag atcaaaaaca agtgcaacac gcctgcccta caccgctgat tgcactgatt
     5521 aatcgggata atggtgcggg cgtgccagac gttggtgagt tgttaaatta cgatgccgac
     5581 aatgtgcagc accgttataa accacaggcg cagccgcttc gtttgatcat tccacggctg
     5641 cacctgtatg ttgcagatta acgcccggct ttcatactgc cgaccatctg ttctggccgt
     5701 acccagctgt caaactcggc ttcgctaaga taccccagcg caagggccgc agcttttaag
     5761 gtcagccctt ctttatgcgc ttttttggcg atctccgcgg ctttgtcata accaatgtgg
     5821 gtgttaagcg cagtcaccag catcagcgat tcattgagta attgattgat tcgctcacga
     5881 ttcggttcaa tacccactgc gcagtgtttg ttaaaacttt ccatgccatc tgccagcaag
     5941 cgcaccgatt gcaggaaatt atggatcacc atcggacgga agacgttcag ttcaaagtta
     6001 ccggaagcgc cacccatgtt gatcgccacg tcgttcccca ttacctgaca gcagagcatg
     6061 gttaatgctt cacactgggt tgggttcact ttccccggca tgattgagct gcccggctca
     6121 ttttccggga ttgagatttc accaattccg cagcgcgggc cagaggccag ccagcggaca
     6181 tcattggcga ttttcatcag tgacgcagcc aaccctttca acgcgccgtg cgcctgaacc
     6241 agggcatcac aggtcgcaag cgcttcaaat ttgttcggcg cggtaacaaa cggtgcacag
     6301 gtaatgactg ccagttcatc tgctacgcga cgcgcatact ccggatgggt atttagtcca
     6361 gtacccaccg ctgtaccgcc aagagccagt tccgctacgt gaggcaggct gtattcgata
     6421 tgtttgagat tatgctcgag catcgctacc cagccggaaa tctcctgccc tagtgttagc
     6481 ggcgtggcgt cctgcaagtg ggttcgaccg attttgacga tatcggcaaa tgcacgcgat
     6541 ttttcactca gtgtctgtgt cagggtttta agctgcggaa tgagttgctt gcgcagcgcc
     6601 agcagcgccg caacgtgcat cgccgtcgga aagacatcgt tggaactttg gcttttgttc
     6661 acgtcgtcgt tagggtgaac tttacgttcc atcccgcgca cgccgccgag taattcactg
     6721 gcccggttag ccagcacttc gttcatgttc atgttacttt gcgtgccgga gccggtctgc
     6781 cagatagcca gcgggaattc gtcgtcatgc tgtcctgcca gtacttcatc cgccgcctgc
     6841 cgaatggcgc tcgctttctc ttcagacaac aagcctaaat cttcattaac ttttgccgct
     6901 gcgcgcttgg ttagcgccag cgcatgaatc agtgaggtgg gcattttctc cgtcgaaatg
     6961 cggaaatgct ccagcgagcg ttgagtttgt gcgccccaca gcttatctgc cgggacatca
     7021 atcgccccca tcgaatcttt ttcgctgcgt actgtattca tggcctgctc ctcacctgtt
     7081 taattttttc tttctgtttt gctttcgtta agcaactttt agctcactca ttatttacta
     7141 tttgataaca aatgtttggt ctttcgtgcc atgtaaaaaa accgccccga agggcggctc
     7201 tgtttatttc acacagcggg tgcattgtgt gagttgtatc tgctggaaga agtcatttcc
     7261 tttatcatcc acaaggataa acgccgggaa atcttccact tcaattttcc agatggcttc
     7321 cattcccagt tccggatatt caacacattc caggctctta atacttccct gcgccaatac
     7381 agcggccgga ccaccgatac tgccgaggta gaagccgccg tgttttttac aggcatccgt
     7441 cacctgctgg ctgcggttgc ctttcgccag catgatcata cttccgccct gcgcttgcag
     7501 ttgatcgaca taagaatcca tccgtccggc ggtcgttggg ccaagagaac cggaagcata
     7561 accttccggc gttttggccg gacccgcgta gtaaatcgga tgatctttaa tgtactgcgg
     7621 cagcccttca ccattatcca tccgctcttt cagtttggcg tgagcaatat cacgaccgac
     7681 gataatcgtg ccgttaagcg ataagcgtgt agaaacggga tactgcgaca actgtgcgag
     7741 gatctctttc atcggacggt taaggtcaac gcgcaccgct tcgccttctc ccgctttgcg
     7801 cagctcttcc gggatatatt tgcctggatt atgttccagt ttttcgatcc agatcccctg
     7861 acggttgatc ttcgctttga tattacggtc agcagagcag gagacgccca taccgaccgg
     7921 gcaggatgcg ccgtgacgtg gcaggcgaat cacgcggatg tcgtgagcga agtatttacc
     7981 accaaactgc gcacccagac cgagattttg cgcttcgatc agcaattctt tttccagttc
     8041 cacatcgcgg aatgcctgac cgtgctcatt cccttccgtt ggcagttcgt catagtattt
     8101 agcggaagcc agtttcactg ttttaaggtt cgtttctgca gaagttccac caataacgaa
     8161 cgcaatatga tacggaggac aggccgccgt acccagcgtg cgcatcttct caaccaggta
     8221 atttttcagt ttccccggcg ttagtaacgc tttggtttcc tgatagagat acgtcttgtt
     8281 tgccgaaccg ccacctttgg cgatacagag gaatttgtac tcgtcgccat caacggcata
     8341 aagatcgatc tgcgctggta gattggtgcc ggtattcacc tctttataca tatccagcgg
     8401 cgcgttttgc gagtagcgca gattatcttc gatataagtg ttatagacac cgcgtgccag
     8461 cgccgcttca tcaccgccac cggtccatac acgctgccct tttttaccaa caataatcgc
     8521 ggtgccggta tcctgacagg ttggcagaac gcctttcgcc gcgatgtcgg agttgcgcag
     8581 gaattgcagc gccacatatt tatcattttc gctggcttcc gggtcacgca gaatgtcggc
     8641 cacctgttgt tggtgagccg gacgcagcat aaatgacgca tcatgaaacg cctgacgcgc
     8701 caacagagtt aacgcttcgg gtgcgacttt caaaatctcc tgcccttcaa attcagatac
     8761 gctaacgtgt tcgctggtta gcaggtaata ctcagtatca tcttttttga gtgggaaagg
     8821 agcctgataa tgaaagggtt tgtttgacat tattctctca cttactgcct ggtttggtta
     8881 tgctctgggc gggtgttccg ttgccctgtt aaaagcgagt aacaatatcc tacacacttt
     8941 tttaacaaaa actgagacta gtacgacttt ttgcggctcc aggttacttc ccgtaggatt
     9001 cttgctttaa tagcgggatt aatttccaca ttaaaacagg gattgatcat gcaaaaactc
     9061 attaactcag tgcaaaacta tgcctggggc agcaaaacgg cgttgactga actttatggt
     9121 atggaaaatc cgtccagcca gccgatggcc gagctgtgga tgggcgcaca tccgaaaagc
     9181 agttcacgag tgcagaatgc cgccggagat atcgtttcac tgcgtgatgt gattgagagt
     9241 gataaatcga ctctgctcgg agaggccgtt gccaaacgct ttggcgaact gcctttcctg
     9301 ttcaaagtat tatgcgcagc acagccgctc tccattcagg ttcatccaaa caaacacaat
     9361 tctgaaatcg gttttgccaa agaaaatgcc gcaggtatcc cgatggatgc cgccgagcgt
     9421 aactataaag atcctaacca caagccggag ctggtttttg cgctgacgcc tttccttgcg
     9481 atgaacgcgt ttcgtgaatt ttccgagatt gtctccctac tccagccggt cgcaggtgca
     9541 catccggcga ttgctcactt tttacaacag ccaaatgctg aacgtttaag cgaactgttc
     9601 gccagcctgt tgaatatgca gggtgaagaa aaatcccgcg cgctggcgat tttaaaatcg
     9661 gccctcgata gccagcaggg tgaaccgtgg caaacgattc gtttaatttc tgaattttac
     9721 ccggaagaca gcggtctgtt ctccccgcta ttgctgaatg tggtgaaatt gaatcctggc
     9781 gaagcgatgt tcctgttcgc tgaaacaccg cacgcttacc tgcaaggcgt ggcgcttgaa
     9841 gtgatggcga actccgataa cg
//
LOCUS       DAJOWB010000152         9842 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJOWB010000152 DAJOWB010000000
VERSION     DAJOWB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.152"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            46..1299
                     /locus_tag="OHO85_004170"
     CDS             46..1299
                     /locus_tag="OHO85_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2673461.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1696..2031
                     /locus_tag="OHO85_004171"
     CDS             1696..2031
                     /locus_tag="OHO85_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCQ2673462.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQP
                     AKPAAQPAA"
     gene            2124..2207
                     /locus_tag="OHO85_004172"
     CDS             2124..2207
                     /locus_tag="OHO85_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673463.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            2307..3128
                     /locus_tag="OHO85_004173"
     CDS             2307..3128
                     /locus_tag="OHO85_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCQ2673464.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIIPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(3167..3496)
                     /gene="mdtI"
                     /locus_tag="OHO85_004174"
     CDS             complement(3167..3496)
                     /gene="mdtI"
                     /locus_tag="OHO85_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCQ2673465.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(3483..3848)
                     /gene="mdtJ"
                     /locus_tag="OHO85_004175"
     CDS             complement(3483..3848)
                     /gene="mdtJ"
                     /locus_tag="OHO85_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCQ2673466.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIVGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            4260..5294
                     /gene="tqsA"
                     /locus_tag="OHO85_004176"
     CDS             4260..5294
                     /gene="tqsA"
                     /locus_tag="OHO85_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCQ2673467.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     SVLLSDLNKE"
     gene            complement(5319..6707)
                     /gene="pntB"
                     /locus_tag="OHO85_004177"
     CDS             complement(5319..6707)
                     /gene="pntB"
                     /locus_tag="OHO85_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCQ2673468.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(6718..8250)
                     /gene="pntA"
                     /locus_tag="OHO85_004178"
     CDS             complement(6718..8250)
                     /gene="pntA"
                     /locus_tag="OHO85_004178"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCQ2673469.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            8774..9718
                     /locus_tag="OHO85_004179"
     CDS             8774..9718
                     /locus_tag="OHO85_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCQ2673470.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
BASE COUNT         2461 a         2552 c         2354 g         2475 t
ORIGIN      
        1 ctatgtataa gcctgatcca caggcatatt tagcaaggat ttcaagtgag ccgtactaca
       61 actgttgatg gcgctccggc aagcgacact gacaagcaaa gcatttctca gccaaatcaa
      121 tttattaaac gcggtacgcc gcaatttatg cgcgtcaccc tggcgctgtt ctctgccgga
      181 ctggcaacat ttgcacttct ctattgtgtg cagcctatcc ttccggtgct ttcgcaggag
      241 tttggcttaa cgcccgcgaa cagtagtatt tcactgtcca tttccacggc gatgttggct
      301 attggtttgc tgtttactgg ccccctatcc gatgccattg gtcgcaaacc agtgatggtc
      361 acggcgctac tgttggcctc catttgtacg ttactttcga caatgatgac cagctggcac
      421 ggcattttga ttatgcgcgc cttgattggg ctttcgttaa gtggcgtggc agctgttggc
      481 atgacttatc ttagcgagga aatccacccc agtttcgtgg ccttttcgat ggggttgtat
      541 atcagcggca actcaattgg cggcatgagc gggcgcttaa ttagtggtgt cttcacggac
      601 tttttcaact ggcgaattgc tctggcggca atcggttgtt tcgcgctagc ctcggcgttg
      661 atgttctgga aaatcctccc tgaatcacgc cattttcgcc cgacttcgct gcgacctaag
      721 acgttgttta tcaactttcg tctgcactgg cgtgaccggg gattaccgtt attgttcgca
      781 gaaggctttt tgctgatggg gtcgttcgtc acgctgttta attacatcgg atatcggttg
      841 atgctctccc cctggcatgt cagtcaggcc gtggttggct tattatcgct ggcttatttg
      901 accggtacat ggagctcacc caaagccgga accatgacca cccgctacgg gcgcggtccg
      961 gtgatgttgt tttcgacggg ggttatgctg tttggtttac tgatgacctt attcagctcg
     1021 ctgtggctga tctttgccgg aatgttactc ttctcagcag gattcttcgc agcccactca
     1081 gttgccagca gctggattgg cccccgcgca aaacgcgcta aaggccaggc ctcctcgctg
     1141 tatctgttca gttactatct ggggtcgagt attgccggga cgctgggtgg tgttttctgg
     1201 cataactatg gctggaacgg cgtcggcgca tttattgctc tgatgctggt cattgctctg
     1261 ctggtcggga cgcgtttgca tcgtcgtctg cacgcctgaa aaataagccc ggactgcggt
     1321 aactacacgt ccggacttat tgcttgctca aaccaacgtt aatagccatc ctaaaataga
     1381 cgaagcgcca gccaattccc gcagcgcgtc tagcgtcatc aggattataa gtacccaaat
     1441 aaacggattc attttgctgt gtgtcattta ttactgatgc gcagttattc taccgctttg
     1501 taagtagtaa aatagttaac ccgatcaaga ctactattat tggtagctaa atttccctta
     1561 agtcacaata cgttattatc aacgctgtaa tttattcagc gtttgtacat atcgttacac
     1621 gctgaaacca accactcacg gaagtctgcc atttccaggg atatagttat ttcaacggcc
     1681 ccgcagtggg gttaaatgaa aaaacaaatt gagggtatga caatgaaaaa agtattagct
     1741 ctggttgttg ccgctgctat gggtctgtct tctgccgcct ttgctgcaga gactgcgacc
     1801 acacctgctc cgactgcgac gaccaccaaa gcagcgccgg cgaaaacgac acatcataaa
     1861 aaacagcata aagcagcacc tgcccagaaa gcgcaggcgg ctaaaaagca tcataaaaat
     1921 acgaaagctg aacagaaagc ccctgaacaa aaagcgcagg cagcgaagaa acacgccggg
     1981 aaacacagcc atcagcaacc ggcaaaacct gctgcacaac ccgcagcgta agttttcaac
     2041 agtaatgctg gcgcgccccc tcgcgcctga aaattacggt gctaagcggg taacgtttag
     2101 caccgccttt agcggagggt agtatggtgg gccgttatcg ctttgagttc attcttatca
     2161 tccttatttt atgcgcactg attgccgccc gtttttatct ttcctgactg tagttatctg
     2221 attttactcc cactttcatc ccgtcccgtc tatagtattt atgagggttt gcttttaata
     2281 atcataatta cccaccagag tgtgatatgc gtacaaccat tgctgtagtg ttgggtgcaa
     2341 ttagtttgac gtctgctttt gtgtttgcag ataaaccaga cgttgccaaa tcggcaaacg
     2401 atgaggtcag caccctgttt tttggtcatg atgatcgtgt gccagtgaat gacacgaccc
     2461 aatcaccgtg ggatgcggtt gggcaactgg aaacggccag cggcaattta tgtacggcga
     2521 cgctgattgc acccaatctg gcattaacgg caggacactg tttattgacg ccgcctaagg
     2581 gtaaagcgga taaggcggta gcgctgcgtt ttgtgtcaaa taaaggtctt tggcgctatg
     2641 acatccacga catagaaggc cgcgttgatc cgacactggg aaagcggtta aaagcagatg
     2701 gggatggttg gattattcct cccgcagctg caccatggga cttcggcttg attgtgctgc
     2761 gtaatccccc ttctggcatt acaccgttgc cgttatttga gggagataaa gccgcgctta
     2821 ctgccgcatt aaaatcggct ggtcgtaaag tgactcaggc gggatatcct gaagatcatc
     2881 tcgatacgtt atacagtcat caaaactgtg aagtgactgg ctgggcgcaa acgtcggtga
     2941 tgtcacatca gtgcgatacc ttgccgggtg acagcggttc gcctctgatg ttgcataccg
     3001 atgacggctg gcaattaatt ggggtgcaaa gttcggctcc tgccgcgaaa gatcgctggc
     3061 gcgccgataa ccgggccatt tctgttaccg gttttcgcga caagctggat caactgtcgc
     3121 aaaaataatg ttcaaacgct gcccgacagt gcgggcagcg tgttcatcag gcaagtttca
     3181 ccatgatcat tccagccaac agcaagacca ggccaatcca gcctttacga tttaaccgtt
     3241 gaccaaacaa gatccaacct gcggctaacg tggcggcaat accaaacccg ccccacaatg
     3301 cataagcgac agacaagtcg atccctttta cggcctgaga aagcgcacta aaggcagcca
     3361 gcaccgccgc cagggagagc aagccaaata ttttgcgacg aaagccgtca gaaaatttca
     3421 aaaagacgtt agcaacgatt tccagcacga ttgccaatgc cagccaggcg gcgtgaaccc
     3481 attcaaactg cgccatggtt cacctccagt tcaggtttac gcgctttacg ggtacctgat
     3541 ttgatcaaca caatcccggc gaccagggtg gttaacccga caattttcat cagcgataaa
     3601 ctttcgtcga ataacaaaac gctaaacaag gtaataaata aaataccgat accttcccac
     3661 agcgcataag ctacgcctaa ggcgatcttt ttaacggcga aagagagaaa tatatacgac
     3721 agagaaatca tcaccagcat taaaataaag ccgccatttc cctcactgac gctcgcccat
     3781 ttcattgaca gcgtaccggt aatttctgta gcaatagcca ggcctaataa aatccaataa
     3841 atatacattg tccttctcct gcaagagaat tattttaatt ttcgcttaat tcagcgaaac
     3901 caaagtaaag tggtcaagcc ccgcgcatag ggcaagctta ggcagaagaa aggactaaag
     3961 cgcgttgcgc cagtgctgct cacctacgag caagatagtg gatgaggtac gaagatgggt
     4021 aaatgttgaa aataacgtcc tgaacaaatt gtccataata ttacaattat ccgcagtgtt
     4081 gcttctcgtc atcgcggatg ataattgtcc tcggtagttg aacacgcctg atttgtatca
     4141 tagcttaaga attaactcaa aatattttca cttctttacc tgagcggttt gattttcgtt
     4201 atgatgacgg agcgaaaaag acattattat tagcaaagga agaaaaaacg gggacaagca
     4261 tggcaaagcc gatcatcacg ctcaatggcc taaaaatcgt cattatgttg ggaatgctgg
     4321 tcattattct ctgcggtatc cgttttgccg ccgagatcat cgtgccgttt attctcgcat
     4381 tatttattgc tgttattctt aacccgctgg tgcaacacat ggtccgctgg cgagtgccgc
     4441 gtgtactggc ggtgtcgatt ttgatgacca tcatcgtgat ggcgatggtg ttgctgttag
     4501 cttatctggg ttccgcgctc aacgagttga cgcggacgtt accgcaatat cgcaactcta
     4561 ttatgacgcc gctgcaagct cttgagccgt tgttgcaacg cgtagggatt gacgtctcag
     4621 ttgaccaact ggcgcattac attgatccga acgcggcgat gacgttgctc accaacttat
     4681 tgacgcagtt atctaatgcc atgtcatcaa tatttttatt gctgctgacg gtgctgttta
     4741 tgctgctcga agtgccacaa ttgcccggaa aatttcagca aatgatggcg cgtccggttg
     4801 aagggatggc ggcgattcaa cgtgcgattg acagtgtttc tcattatctg gtgctgaaaa
     4861 cagccatcag catcatcacc ggcctggtcg cctgggcgat gctcgccgca ctcgatgttc
     4921 gcttcgcttt tgtctgggga ttgctggcct ttgcgcttaa ttacatccct aatattggtt
     4981 cagttctcgc ggcaatcccc cctatcgctc aggtactggt gtttaatggc ttctacgaag
     5041 cgttgctggt gctggcggga tatctgctga ttaatctggt cttcggcaat attctggagc
     5101 cgcgcatcat gggacgtggg ctggggcttt cgacattggt ggtatttttg tcgttgattt
     5161 tttggggatg gttgttagga ccggtgggta tgctgctttc cgtgccgttg acaattattg
     5221 tcaaaattgc gcttgaacaa acagcgggag gtcaaagcat ctccgttctg ttaagcgatc
     5281 tcaataaaga gtgacggcct ccgcagaggc cgtcagggtt acagagcttt caggattgca
     5341 tccacgctgg ctttggcgtc accaaacagc atgtgggtgt tttccttgaa gaacagcggg
     5401 ttttgcacac cagcatagcc agtgttcatc gaacgtttaa agacaatcac gttctgcgct
     5461 ttccacactt ccagcacagg cataccagca ataggactct tcggatcatc ctgcgccgcc
     5521 gggttaaccg tatcgttagc accaatcacc agtacggtat cggtatcagc aaagtcatcg
     5581 ttgatctcgt ccatttccag cacgatgtca tacggtactt ttgcttcagc cagcaatacg
     5641 ttcatatgtc caggcaaacg ccccgcaacc gggtggatac cgaaacgcac attaatacca
     5701 cgagcgcgca atttctcagt aatttcagcg acaggatatt gcgcctgcgc aactgccatg
     5761 ccgtaccccg gagtaatgat cactgaatgg gagtttttca gcagttccgc tgtctcttct
     5821 gcagtgattt cgcggtgctc gcccacttcc tgatcatcac cagtagaaga gccgtcggtg
     5881 ccgaaaccac ccgcaataac gctgataaag gaacggttca tcgccttaca cataatgtaa
     5941 gaaaggatag cccccgaaga accgaccagc gcaccggtca caatcagcag gtcgttgctg
     6001 agcataaagc ccgcagccgc agccgcccag ccggagtacg agttcagcat cgacaccacc
     6061 actggcatat ctgcaccacc gatggaggcg actaaatgcc agccgaatac cagcgcaatg
     6121 gcggtcatta tcagcaatgc cagcacttgc aggccgacgc tatccgtgcg aacaaataca
     6181 atcagcagca ggaaggaaac gaccagagcc gccaggttca ttttgtgacg gtttggcagc
     6241 atcaacggtt tagacgaaat cttgccacac agtttgccga acgccaccac cgaacccgtg
     6301 aacgttaccg ccccgatgaa gataccgagg aacacttccg tcaggtgaat attgaccaga
     6361 atcggtgcca ttcccgcgtc atgatgcaga tagctgttaa agccaaccag cactgccgcc
     6421 agacccacga agctatgcag gatcgccacc agttctggca tttcggtcat ttcaactttc
     6481 ttcgccagac ggataccaat tgccccacca atgaccatcg ccagcaagat ccagccaaca
     6541 ttacccgtat ccggtccaaa aatggttgcg attaacgcaa tcgccatccc ggcgataccg
     6601 aagttgttac cctggcgaga cgtttcatgt ttcgaaagac cggccagact gaagataaac
     6661 aggatcgcgg caacaatgta tgcagctgta actaatcctc cagacatatg ttacccctta
     6721 atttttgcgg aacattttca gcatgcgctg agtcacggtg aagccaccga aaatattaat
     6781 gctggctata agcaccgcga taaaactaag gaagctaacc cagccgccct ggccaatctg
     6841 caacagtgct ccgacaacaa taatccctga aatcgcgttg gtgaccgaca tcaacggtgt
     6901 atgcagcgca tgcgatacat tccacaccac gtaataaccg acaacgcagg ccagcgcgaa
     6961 aacggtgaag tgcccaagaa actctttcgg cgcaacgctt gccagccaac cgaaaaggat
     7021 gattgccagc gccatcaatg cgtatttacg ccacggtgag caggcacatt tttcctcagt
     7081 tttcacttcc ggtgccgctt tttgtgccgc ctgcggctga gctgatacct gaatcggcgg
     7141 tgccggccag gtaatttcgc ccgcacggat cacggtcacg ccgcgaatca ccacatcatc
     7201 aaaatcaaca gtgatattgc cgtctttctc tttgcacaac agtttcagca gattaacgag
     7261 gttagtaccg taaagctgtg aggattgcgt cggcagacgg cccggaagat cggtataacc
     7321 aatcactttg acaccatttt ccgtagtgaa gatttcaccc ggcacggtgt attcacagtt
     7381 gccgccgttt tgggctgcca ggtcgacaat cacactgccc gccttcatgg agtcaaccat
     7441 ttcacgggta attagcttcg gcgctggttt gcctggaata agcgcggtgg tgacaatgat
     7501 atcgacctct tttgcctggg cggcaaagag ttccatttcc gctttgatga acgcgtccga
     7561 catcactttg gcatagccat cgccgctgcc cgcttcctct ttaaaatcca gctcgaggaa
     7621 ttccgcgccc atactttgaa cttgttcttt cacttccggg cgggtgtcga atgcacgcac
     7681 aatcgcgccg agactgtttg ctgcgccaat ggcggccaga cctgcaacac ccgcaccaat
     7741 caccatcact tttgccggtg gcactttccc ggccgcagta atttgcccgg taaagaagcg
     7801 cccaaattca tgtgccgctt caacaatggc gcgataaccg gcgatgttcg ccatcgagct
     7861 tagtgcgtcc agcgattgtg cgcgtgagat acgcggcaca gagtccatcg ccatcacggt
     7921 cacgttacgt tccgcaagtt tttgcattaa ttccggattc tgcgcaggcc agataaaact
     7981 caccagcgtt gtcccaggat tcagtaacgc aatttcatca tctaacggcg cattgacctt
     8041 cagaatgatc tctgactgcc agacgctatt cccttctaca atttcagcgc ccgcttgcac
     8101 aaacgcttta tcgtcaaaac ttgccagttg acccgcgccg ctctctaccg cgacggtaaa
     8161 acccagtttc agcagctgtt ccactgtttt tggcgttgct gcaacacggg tttcattggt
     8221 taaccgttct cttggtatgc caattcgcat gatattccct tccatcggtt ttattgatga
     8281 tggtttgcct gtgtcaggag ccacacaagc cgctcatgta cgagctaaat gttactccgt
     8341 taaaataaat tagtaacaaa cgccctataa cgtactgaaa attatgcctg tgatctagcg
     8401 ccaaaaatca gtatttcggc gtgaactcgc aaaatattaa cgattcagcc gtgatagtgg
     8461 gataaacacc ttagaacgcc ggataaagac tgataattgt cttcgacggt cgggtaaaac
     8521 gagacatcgc cccggcacgc atcactactt aacattaaat taacttatac aattcagttg
     8581 cttcagtagt aatgatgctg atacggctgt tttttaagca tagacggtca tttgagcagg
     8641 attaaaattg gcttaaggaa tgtgatatga aaaatgacgc agacagttac accgtttaaa
     8701 tgcaataatc agccacgttt ctcgttaata acaataccag tacttggttt gcgcaaggcg
     8761 aaggattatt tttatgaagc ttaagaacac cctcctggcg tcggcactgc tttctgctac
     8821 ggcattctcc gttaacgcag caacagaact gacaccggag caagcggcag cggttaaacc
     8881 ttttgaccgt gtagtggtta ccggtcgttt taatgctatt ggcgaagcgg tgaaagccgt
     8941 ttctcgtcgc gcagataaag aaggtgccgc ctctttttat gttgtcgaca cttctgattt
     9001 tggtaacagc ggtaactggc gtgtggtcgc tgacctctat aaagccgatg ctgaaaaagc
     9061 agaagaaaca agtaatcgcg taattaacgg tgttgtcgaa ctgccgaaag atcaggctgt
     9121 tctgattgaa ccgtttgaca cggtcaccgt ccagggcttc tatcgtagcc agccagaagt
     9181 caatgatgcc atcaccaaag cagcaaaagc gaaaggtgcc tactctttct acatcgttcg
     9241 tcaaatcgat gccaaccagg gcggcaacca gcgtattact gcattcatct ataaaaaaga
     9301 tgctaagaaa cgtatcgtcc agagcccgga tgtgatcccg gcagattccg aagcaggacg
     9361 tgcagctctg gctgccggtg gcgaagccgc gaagaaagtt gagatcccgg gtgttgcgac
     9421 taccgcatca ccaagttctg aagtcggtcg cttctttgaa acccagtcat caaaaggcgg
     9481 gcgttacacc gtcacgctcc cggatggcac taaagtcgaa gaactgaaca aagcgaccgc
     9541 agcgatgatg gtcccgttcg acagcatcaa attctctggc aactacggca acatgaccga
     9601 agtctcctat caggttgcga aacgtgccgc gaagaaaggt gctaagtatt accacatcac
     9661 ccgccagtgg caggaacgtg gtaataacct gaccgtcagc gcagatctgt ataaataact
     9721 attcagttac tcctataagg cggcttgatg ccgcctttcg catttttagt cacaaatttc
     9781 cccgcgacca ttgcatgcag acacatctct tcgtaaaatc ccgcgcttca gtggcaaact
     9841 cc
//
LOCUS       DAJOWB010000153         9755 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJOWB010000153 DAJOWB010000000
VERSION     DAJOWB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.153"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            119..896
                     /locus_tag="OHO85_004180"
                     /pseudo
     CDS             119..896
                     /locus_tag="OHO85_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155535.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
     gene            898..1167
                     /locus_tag="OHO85_004181"
     CDS             898..1167
                     /locus_tag="OHO85_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HCQ2673471.1"
                     /translation="MDTILLTGLFAAFFTTFAFAPQSIKTIRTRNTEGISVVMYIMCL
                     TGVISWIAYGIMRSDFAVLIANIVTLFLAAPVLVITLINRRKKHV"
     gene            1164..2144
                     /locus_tag="OHO85_004182"
     CDS             1164..2144
                     /locus_tag="OHO85_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004172011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HCQ2673472.1"
                     /translation="MNIAIIGAGPAGIISARNAIKAGHSVVLFEKNTRIGGIWNPWSG
                     GAYRNACMQNSRYTFHYTGFPPGDIDEFPGVEQVFRYLSAVAGEDALRESIRLNTEVV
                     SLRKDAGHRVIRCASEGKDTEDIFDRVIIATGELWQPRRPPLPGEENFSGTLITSRDY
                     QEPEAFKGKNILIIGGGVSGADIASDLVPFARSVSLSVKKMGLYLPRQFPTGPNDMMH
                     SYLGRCLLSQMNYEDFIGYLDTMMPDYMQAYRASSLLPDMANNNAVHVNEKIIPNVAA
                     GLIKVKPQAERFTGEGAIKFVDASQEKYDVIITCTGYEMPDYSFIRKRTA"
     gene            2211..2600
                     /locus_tag="OHO85_004183"
     CDS             2211..2600
                     /locus_tag="OHO85_004183"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2673473.1"
                     /translation="MSDMQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALE
                     NGINANLLFKWRQQWREGKLLLPSSESSQLLPVTLDAAAEQPESLAEDPETLSISCEV
                     TFRHGTLRFNGNVSEKLLTLLIQELKR"
     gene            2597..2944
                     /gene="tnpB"
                     /locus_tag="OHO85_004184"
     CDS             2597..2944
                     /gene="tnpB"
                     /locus_tag="OHO85_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCQ2673474.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            2994..4268
                     /locus_tag="OHO85_004185"
                     /pseudo
     CDS             2994..4268
                     /locus_tag="OHO85_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            4308..4574
                     /locus_tag="OHO85_004186"
                     /pseudo
     CDS             4308..4574
                     /locus_tag="OHO85_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722340.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
     gene            complement(4561..5012)
                     /gene="def"
                     /locus_tag="OHO85_004187"
                     /pseudo
     CDS             complement(4561..5012)
                     /gene="def"
                     /locus_tag="OHO85_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553770.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
     gene            complement(5121..9254)
                     /gene="hbp"
                     /locus_tag="OHO85_004188"
     CDS             complement(5121..9254)
                     /gene="hbp"
                     /locus_tag="OHO85_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021534952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-binding protease autotransporter Hbp"
                     /protein_id="HCQ2673475.1"
                     /translation="MNRIYSLRYSAVARGFIAVSEFARKCVHKSVRRLCFPVLLLIPV
                     LFSAGSLAGTVNNELGYQLFRDFAENKGMFRPGATNIAIYNKQGEFVGTLDKAAMPDF
                     SAVDSEIGVATLINPQYIASVKHNGGYTNVSFGDGENRYNIVDRNNAPSLDFHAPRLD
                     KLVTEVAPTAVTAQGAVAGAYLDKERYPVFYRLGSGTQYIKDSNGQLTKMGGAYSWLT
                     GGTVGSLSSYQNGEMISTSSGLVFDYKLNGAMPIYGEAGDSGSPLFAFDTVQNKWVLV
                     GVLTAGNGAGGRGNNWAVIPLDFIGQKFNEDNDAPVTFRTSEGGALEWSFNSSTGAGA
                     LTQGTTTYAMHGQQGNDLNAGKNLIFQGQNGQINLKDSVSQGAGSLTFRDNYTVTTSN
                     GSTWTGAGIVVDNGVSVNWQVNGVKGDNLHKIGEGTLTVQGTGINEGGLKVGDGKVVL
                     NQQADNKGQVQAFSSVNIASGRPTVVLTDERQVNPDTVSWGYRGGTLDVNGNSLTFHQ
                     LKAADYGAVLANNVDKRATITLDYALRADKVALNGWSESGKGTAGNLYKYNNPYTNTT
                     DYFILKQSTYGYFPTDQSSNATWEFVGHSQGDAQKLVADRFNTAGYLFHGQLKGNLNV
                     DNRLPEGVTSALVMDGAADISGTFTQENGRLTLQGHPVIHAYNTQSVADKLAASGDHS
                     VLTQPTSFSQEDWENRSFTFDRLSLKNTDFGLGRNATLNTTIQADNSSVTLGDSRVFI
                     DKNDGQGTAFTLEEGTSVATKDADKSVFNGTVNLDNQSVLNINDIFNGGIQANNSTVN
                     ISSDSAVLGNSTLTSTALNLNKGANALASQSFVSDGPVNISDATLSLNSRPDEVSHTL
                     LPVYDYAGSWNLKGDDARLNVGPYSMLSGNINVQDKGTVTLGGEGELSPDLTLQNQML
                     YSLFNGYRNIWSGSLNAPDATVSMTDTQWSMNGNSTAGNMKLNRTIVGFNGGTSPFTT
                     LTTDNLDAVQSAFVMRTDLNKADKLVINKSATGHDNSIWVNFLKKPSNKDTLDIPLVS
                     APEATADNLFRASTRVVGFSDVTPILSVRKEDGKKEWVLDGYQVARNDGQGKAAATFM
                     HISYNNFITEVNNLNKRMGDLRDINGEAGTWVRLLNGSGSADGGFTDHYTLLQMGADR
                     KHELGSMDLFTGVMATYTDTDASADLYSGKTKSWGGGFYASGLFRSGAYFDVIAKYIH
                     NENKYDLNFAGAGKQNFRSHSLYAGAEVGYRYHLTDTTFVEPQAELVWGRLQGQTFNW
                     NDSGMDVSMRRNSVNPLVGRTGVVSGKTFSGKDWSLTARAGLHYEFDLTDSADVHLKD
                     AAGEHQINGRKDSRMLYGVGLNARFGDNTRLGLEVERSAFGKYNTDDAINANIRYSF"
     gene            9425..9616
                     /locus_tag="OHO85_004189"
                     /pseudo
     CDS             9425..9616
                     /locus_tag="OHO85_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(9587..9755)
                     /locus_tag="OHO85_004190"
                     /pseudo
     CDS             complement(9587..9755)
                     /locus_tag="OHO85_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865085.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         2440 a         2554 c         2334 g         2427 t
ORIGIN      
        1 actaacgata atgattatcg ttaacatagt caagagtgaa gggtaggagg ccctcaaccc
       61 cctataaggg gtccgcttgg aaaacggatt tccccacgtc aagagaattg atttgaacga
      121 gtggcttcgc tacaaacagc tcccgtttgt gtgtgaaaaa cccctctcaa cagatctcaa
      181 ctctgcacga tatctacagg agattgctcc aaaaggcagt atcgtgtctt ttaactaccg
      241 atataacccc gtcatcgaca tgataatgcg tctgagaaat gagaagaaat taggtgaact
      301 gcatttcttc tcagcagaat ttaataaaaa ttctgcactg acccgccatc acctgacctg
      361 gcgagactcc gcacaacaga gcaaaagcag cggtgctctg ggcgatctat cctgtcatct
      421 gcttgattta ttttgtttta taggggaaag cccggtagtg gttcatggga tcaaaacggt
      481 aaaggggaca cgtgggtaca aaatcagatg gtcaggttga agtggatgat aacggctatg
      541 taatgggcag ttcagaaaag ggagcttatt tcagagttca tgccagtaaa tcggaaacag
      601 atcataattt gggactgcat atacagcttg tatttgaaaa tggtgaaatc agatattcaa
      661 cccaccatga aaatcgcctg ctcctgattc tatttaatga tacgaatacg gaaacaattg
      721 gcttcgacgc tctgaaacgc ttgcctgatc caccacggga gcttccattc tggtctgatt
      781 cgtttattca tcttcatgac gactggtgtg ctcttattaa atatggtcac tcgtctccta
      841 agctggcgga cctgtcatcc ggccttcata tacaggaaat aattgaggca ttctgatatg
      901 gataccattc ttttaaccgg gctgtttgct gcatttttca caacgtttgc gttcgctccc
      961 cagagcataa aaactatcag gacacgcaat accgaaggta tctccgttgt tatgtatatc
     1021 atgtgcctga caggtgtaat ctcatggata gcgtatggca ttatgcgatc tgattttgcc
     1081 gtactgatcg ccaatattgt cacactgttt ctggctgcac cagtgcttgt aataacactt
     1141 atcaaccgca ggaaaaaaca cgtatgaata ttgcaatcat cggcgcgggt cctgccggca
     1201 tcatttctgc ccgtaatgcg attaaagcag gtcactccgt ggttctgttt gagaagaata
     1261 cccgaattgg ggggatctgg aacccctgga gtggtggcgc ttaccgtaat gcctgtatgc
     1321 agaactctcg ttatacattt cattatactg gctttccccc tggcgatatc gatgaatttc
     1381 caggagtgga acaggtattc agatatcttt cggccgtggc cggagaggat gccctccgtg
     1441 agtcgatccg actgaatact gaagttgttt cactcagaaa agacgccgga caccgggtga
     1501 tccgctgcgc ttctgaagga aaagacacgg aagacatttt tgaccgggtc atcattgcaa
     1561 caggtgaact ctggcaaccc cgccggcccc ccctgccagg tgaggaaaac ttctccggaa
     1621 cgttgatcac gtcgagagat tatcaggagc cagaggcatt taaaggaaaa aatatcctca
     1681 tcattggcgg cggtgtcagc ggtgcggata ttgcctcaga ccttgttccc tttgccagaa
     1741 gcgtaagtct gtccgtcaaa aagatgggac tttatctgcc gagacaattc ccgactggcc
     1801 cgaatgacat gatgcactcc tatctgggca ggtgtctgct gagccaaatg aattatgaag
     1861 attttatcgg ttatctcgac accatgatgc ctgactacat gcaggcctac cgagcatcca
     1921 gtctgttgcc cgacatggcg aacaataacg cggtgcatgt taacgaaaaa atcataccga
     1981 atgtggccgc cggtctgata aaagttaaac cccaggcaga gcgtttcacc ggagaaggcg
     2041 ctattaagtt cgtcgacgca tctcaggaaa aatatgatgt aataataacc tgtacaggct
     2101 atgaaatgcc tgattactcg ttcattcgta agcgtacagc ctgaaccgtc tggtcagaat
     2161 ctgacgaatt agacaaagtg gtgtccacca aataagtagt gggaaccaaa gtgtcagata
     2221 tgcagaaaaa tgtgactccc ggcaggcgaa aaggctgccc taattatcct cccgaattta
     2281 aacagcagct cgttgctgcc tcctgtgaac ccgggatatc catctcaaaa cttgctcttg
     2341 aaaatggcat taacgccaat ctgttgttca aatggcgaca acaatggcgc gagggaaagc
     2401 tgctattacc ttcttcagag agctcccagc tacttcctgt gactctcgat gcagctgccg
     2461 aacagccaga atcgcttgca gaggacccgg aaaccctcag tatcagctgt gaggtaacgt
     2521 tccggcacgg gacgctccgc ttcaatggca atgtcagcga aaagctcctg actctgctga
     2581 tacaggaact gaagcgatga tcccgttacc ttccgggacc aaaatttggc tggttgccgg
     2641 tatcaccgat atgagaaatg gcttcaacgg cctggctgcg aaagtacaga cggcgctgaa
     2701 agacgatccc atgtccggcc atgttttcat tttccggggc cgcagcggca gtcaggttaa
     2761 actgctgtgg tccaccggtg acgggctgtg cctcctgacc aaacggctgg agcgtgggcg
     2821 cttcgcctgg ccgtcagccc gtgatggcaa agtgttcctt acgcaggcgc agctggcgat
     2881 gctgctggaa ggtatcgact ggcgacagcc taagcggctg ctgacctccc tgaccatgct
     2941 gtaaatctct ttatcctggt tgtcacagaa taagcccggt aaaatacggg cttatgaacg
     3001 acatctcttc tgacgacatc ttcctgctga aacagcgcct ggccgaacag gaagcgctga
     3061 tccacgccct gcaggaaaag ctgagcaacc gggagcgcga aatagaccat ctgcaggcgc
     3121 agctggataa actccgccgg atgaacttcg gcagtcgttc cgaaaaagtc tcccgccgta
     3181 tcgcacaaat ggaagccgat ctgaaccggc ttcagaaaga gagcgatacg ctgactggta
     3241 gggtgtatga cccggcagta cagcgtccgt tgcgtcagac ccgcacccgt aagccgttcc
     3301 ctgaatcact accccgtgac gaaaagcgac tgttgcctgc ggcgccgtgc tgcccgaact
     3361 gcggcggttc actgagctat ctgggcgagg ataccgccga acagctggag ttgatgcgta
     3421 gcgccttccg ggttatccgg acggtacggg aaaaacatgc tccatgccga tgataccccg
     3481 gtccaggtac tgctgccggg taataagaag acgaagaccg ggcggttgtg ggcgtatgtt
     3541 cgtgatgacc gcaatgccgg gtcagcgttg gcacctgcag tgtggttcgc ttacagcccg
     3601 gacaggaaag gcatccatcc gcagactcat cttgcttgct tcagcggtgt gctgcaagcg
     3661 gatgcgtacg ccgggttcaa cgagctgtat cgcaatggtc ggataacgga agctgcctgc
     3721 tgggctcatg cccgccgaaa gatccacgat gtgcacgtcc gcatcccgtc agcactgacg
     3781 gaagaagccc tggagcagat cggtcagttg tacgccatag aggcggatat aaggggaatg
     3841 ccggcagagc agcggcttgc tgaacgtcag cgaaaaacga aaccgctgtt gaaatccctg
     3901 gaaagctggt tgcgtgaaaa gatgaagacc ctgtcgcgac actcagagtt ggcgaaggcg
     3961 ttcgcgtacg cacttaacca gtggccggca ctgacgtact atgcgaacga tggctgggtg
     4021 gaaatcgata acaacatcgc tgaaaatgcc ctgcgggcgg tcagtctggg tcgtaaaaac
     4081 ttcctgttct tcggctctga ccatggtggt ggcggggagc gctactgtac agcctgatcg
     4141 ggacgtgcaa actgaatgac gtggatccag aaagctacct tcgccatgtg cttggcgtca
     4201 tagcagactg gccggtcaac cgggtcagcg aactgcttcc gtggcgcata gcactgccag
     4261 ctgaataaca catccccgtc aatacggccc tcgctgtacg cttaccaata aataatatgc
     4321 ttatcgacct gatggctgca atgtcgcata aagactggct gagtcgtcgt caacgacaaa
     4381 aacagggaat tgaacgtgcc catactttag gtaagtatcg gggcaaacag gcagatcggg
     4441 aacgacatca gaaagtaatg tactaccggc agatcaagaa actgagcatc cgtgaaacgg
     4501 cagaggtgac aggctatagt acttcccaag tttgtcgcat tcaggcgctc tataaagagg
     4561 ttaaacctga ttaaaaaata gccttaacgt acaatatttt ccgtttccca aacggagccc
     4621 aatacagatg gctaacagac cgtctgcttc cagttcaaat ggtttaccgt cgcggtccag
     4681 ggcgcgaatt ttaactttct ctgcgcgtgg cactaaagct cgttgttcag ggattgacag
     4741 gcaaccttct tcaatgcctg tttcgccgct tttttctaaa agctccggat tgattaacac
     4801 cagccgttcg tcacggtttt ccgaaacatc aattacaatg atacgttgat ggatatcaac
     4861 ctgggttgcc gccaggccaa tactttttct gcgtacatcg tctcgaacat atcatcgacg
     4921 atacgctgaa tttctgcatt cacttcttct accggtttag caactttgcg aagccgctcg
     4981 tccggaatat gtaacacttg caaaactgac ataaatctcc agagatgtgt tcaggagtta
     5041 gaaagattat ttcttctact gtaccgtaat cagataatcg cagcaaaggg acaaagcaat
     5101 agtccctttg ctgcacagca tcagaatgaa taacgaatat tagcgtttat cgcatcatct
     5161 gtgttgtatt taccaaatgc agagcgttca acttccagcc ccagacgcgt attgtcgcca
     5221 aaccgggcat ttaaccccac accgtaaagc atacgactgt cttttctgcc attaatctga
     5281 tgttctcccg ctgcatcctt cagatgaacg tcagcactgt ccgtcagatc gaactcataa
     5341 tgcaggccgg cacgggctgt cagactccag tccttaccac tgaaggtttt accggaaaca
     5401 acgccggttc tgcctaccag aggattaacg ctgttacgac gcattgagac atccattcca
     5461 ctgtcgttcc agttaaatgt ttggccctgc agtcttcccc agaccagttc cgcctgaggt
     5521 tcaacaaacg tcgtatctgt cagatgataa cggtatccga cttctgcacc tgcatacagt
     5581 gaatggctgc ggaagttctg tttaccagct ccggcaaagt tcaggtcata tttgttttca
     5641 ttgtgaatat atttggcaat cacatcaaag taagcgccgg accggaacag accactggca
     5701 tagaaaccac caccccatga ttttgtttta ccgctgtaca ggtctgctga cgcatctgtg
     5761 tcagtgtagg tggccatcac gccggtaaac aggtccatac ttcccagttc gtgcttacgg
     5821 tcagccccca tctgcagcag ggtatagtgg tcagtgaaac cgccatcagc agagccggaa
     5881 ccgttcagca gacgcaccca cgtaccggct tcgccattaa tatccctcaa atcgcccatg
     5941 cgtttgttca ggttgttaac ttcagtgatg aagttgttat agctgatgtg catgaatgtg
     6001 gcggcagcct taccctggcc gtcgttacgt gcaacctggt aaccatcgag gacccactct
     6061 tttttcccgt cctcttttct gacactaagg atgggggtga catcactgaa tcccacaacc
     6121 cgtgttgatg ccctgaacag attatcagct gtcgcttcag gtgcgctgac cagtggaata
     6181 tcaagcgtgt ccttgttaga aggttttttc aggaagttaa cccagatgct gttgtcatga
     6241 cctgttgccg acttgtttat caccagtttg tctgccttgt taaggtctgt acgcatgaca
     6301 aatgctgact gaaccgcgtc cagattatct gttgtcagtg tcgtgaacgg tgatgttccc
     6361 ccgttaaaac cgactattgt ccggttaagt ttcatatttc ctgccgtgga gtttccgttc
     6421 atcgaccact gggtgtctgt catgctgacg gtggcatccg gtgcattcag gctcccgctc
     6481 cagatattgc ggtacccgtt aaacaggctg tacaacatct gattctgaag agtcaggtca
     6541 ggactcagtt ccccttcccc tccgagggtg acagtccctt tatcctgaac attgatatta
     6601 cctgacaaca tactgtacgg ccccacgttc aggcgggcat cgtctccctt caggttccat
     6661 gaaccggcat aatcgtatac aggtaaaagt gtgtgagata cctcatcagg acggctgttc
     6721 agactcaggg tggcatcaga aatattcact ggaccgtcag aaacaaaact ctgactggcc
     6781 agagcatttg ctcccttgtt cagattcagg gcggtactgg tcagtgttga gttccccaga
     6841 acggcactgt ctgaggagat attcacggta ctgttgttcg cctgtattcc gccattgaat
     6901 atatcattga tattcagcac tgactgatta tccaggttga cggtgccgtt gaagacactt
     6961 ttatctgcat ctttagttgc aacagatgtg ccttcttcaa gggtaaaggc tgttccctgg
     7021 ccatcgtttt tgtcgataaa tacccggctg tcgcccagcg tgacgctgga gttatctgcc
     7081 tggatggttg tgttcagtgt ggcattgcga ccaagaccaa aatcagtgtt cttcagtgac
     7141 agcctgtcaa aggtaaaact gcggttctcc cagtcctcct gactgaatga cgtaggctga
     7201 gtcagaaccg aatggtctcc actggcagcc agtttgtcag ccacagactg agtattgtat
     7261 gcatggataa ccggatgccc ctgcagcgtc agacgcccgt tttcctgggt gaatgtaccg
     7321 gagatatccg cagctccgtc catcaccaga gcactggtaa cgccttcagg caggcgattg
     7381 tccacattca gattgccttt cagttgtccg tgaaacagat accctgcagt attgaaacgg
     7441 tcagctacca gtttctgtgc atccccctga ctgtgcccca caaactccca ggtggcgttg
     7501 ctgctctggt ccgtggggaa ataaccatag gtgctctgct tcaggatgaa gtaatccgtc
     7561 gtatttgtgt acgggttatt gtatttatat aaatttccgg cagttccttt acctgattcc
     7621 gaccagccat tcagtgctac tttgtcagcc cgcagggcat agtccagcgt gatagtggcc
     7681 cgtttatcaa cgttattcgc cagcacggca ccataatctg ccgccttcaa ctgatgaaac
     7741 gtcagactgt taccattaac atccagtgtg cccccacgat atccccatga gacggtatcc
     7801 ggatttacct gccgctcatc agtcagtacc acggtcggcc ggccactggc aatattaaca
     7861 ctgctgaacg cctgcacctg tcctttattg tccgcctgct ggttcagtac aacctttccg
     7921 tccccgacct tcaggccacc ttcattaata cctgtaccct gtaccgtcag cgtaccttca
     7981 ccaattttat gcaggttatc gcccttaaca ccattaacct gccagtttac ggacaccccg
     8041 ttgtccacaa caataccggc accggtccag gtacttccgt tagaggttgt tactgtgtaa
     8101 ttatcacgga acgtcaggga acccgccccc tgagaaaccg aatccttaag gttaatctga
     8161 ccattctgcc cctgaaatat caggttctta ccagcattca ggtcatttcc ctgctgcccg
     8221 tgcatggcat atgtggtggt tccctgtgtc agcgcaccag ctccggtact gctgttaaag
     8281 ctccactcca gtgcaccacc ttccgatgtt ctgaacgtga ccggggcatc attgtcttca
     8341 ttaaatttct gcccgataaa atccagtgga ataacagccc agttatttcc cctgcccccc
     8401 gcgccattcc ccgcagtaag aacaccgacc agcacccatt tattctgaac agtatcaaaa
     8461 gcaaataaag gcgaaccgct gtcaccggcc tcgccataaa tgggcattgc accattaagt
     8521 ttgtaatcaa aaaccagacc tgaactggtg ctaatcattt ctccattctg ataggatgac
     8581 aggctaccga cagtcccgcc ggtcagccag gaatatgcac ctcccatttt tgtcagctgt
     8641 ccgttactgt ccttaatata ctgagtacca gaccccagac gataaaaaac aggatagcgc
     8701 tccttatcca gatatgcgcc agccactgcc ccctgcgccg tcaccgcagt aggggcaacc
     8761 tctgtcacca gtttatccag ccggggggca tgaaaatcca gtgacggcgc attattccgg
     8821 tccacgatat tgtaacggtt ttcaccatca ccaaagctaa cgtttgtata tcccccgtta
     8881 tgtttcacgc tggcgatata ctgcgggttt atcagtgtcg ccacaccgat ttccgaatcc
     8941 acagcactga aatcaggcat agctgcctta tccagcgtac cgacaaattc tccctgctta
     9001 ttataaatag cgatattcgt tgcccccggg cggaacatcc ccttattttc agcaaaatca
     9061 cgaaataact gatacccgag ttcattattg accgttcccg caagacttcc tgcagagaat
     9121 agtaccggga tcagtaataa aaccgggaaa cacagacgtc tgacagactt atgaacacat
     9181 ttcctagcaa actcagatac ggcaataaag ccccgggcca cagcgctgta gcgaagagaa
     9241 taaattctgt tcatttttaa ttactcctga gaaaaataaa atcagtacta ctcaggcata
     9301 acggataatc accatcatcc tgaggaatcc ccagaaaaag gagacaggca tagcgtgatg
     9361 tgaactactg attctttcac aaattcaggg agagcaccgt tatgcctgca cataaagtat
     9421 gccagaaccg gtggcgaatg tgtgctatgc acaaatggtg aaacagttcc tgagtcgtga
     9481 cccgctcgaa tgcgtgctgt gtggcggccg gatggtatac cggcgtgcca tagcggggct
     9541 gaatgtggac gggctgaaga aaaacgcgcg ggatatcagt ctgataagga ttactttcca
     9601 gccggctgat ggctgacggg cgtaatccca ttcgttcggc cagcgcagac tttgttaacc
     9661 ccgctttttc tctcatttcg tataacattt ctaccatctc cagttctttg tcagcatctt
     9721 catacccttt aacagcttct ggagtattaa gcagt
//
LOCUS       DAJOWB010000154         9726 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJOWB010000154 DAJOWB010000000
VERSION     DAJOWB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.154"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..686)
                     /locus_tag="OHO85_004191"
     CDS             complement(<1..686)
                     /locus_tag="OHO85_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISEc13 family transposase"
                     /protein_id="HCQ2673476.1"
                     /translation="MVLSDCYSWANEQFGHARLGDPRRTRRLVSLASSLAQHAGLSIV
                     KSSQSTAQVEGAYRLIRNPSVSPQAIAEAGFTATVRACEAHPLLLALEDTTTINFSHS
                     TASDDLGNTTTNPKTRGLLAHSVLMYAPDSALPVGLIEQQRWSRATDTYGVKHQRKER
                     PYEEKESYRWQQASERMAERLGEIQKRVITVCDREADIWHYLHYKVSHGQRFVVRAAQ
                     NRRLEEAPGKL"
     gene            932..1366
                     /locus_tag="OHO85_004192"
     CDS             932..1366
                     /locus_tag="OHO85_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673477.1"
                     /translation="MSWKERVEKQISRLPKNTALDVIKKLTEVTTIKYNHNGCFPLYT
                     LPPDACFVIRHTEVERLINLYKKRESHPISPSRMTTPLSRLFWLACKHNDTISPLLNH
                     PYKLLSIFEQWASDDGIGEKLDAETLKNALKRGSPSSTSLSG"
     gene            1506..1691
                     /locus_tag="OHO85_004193"
     CDS             1506..1691
                     /locus_tag="OHO85_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCQ2673478.1"
                     /translation="MTSYQLLRLRQVEQKTGLKRSQIYLYMKEGAFPRSIKIGPASVA
                     WLESEIDEWINKKLSDR"
     gene            1713..1895
                     /locus_tag="OHO85_004194"
     CDS             1713..1895
                     /locus_tag="OHO85_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673479.1"
                     /translation="MNINIPALINFDPAGIKKSTCMYISDLPGIVLYDTIPFTEYSAE
                     LLSTYSVKESISCFHP"
     gene            1880..2461
                     /locus_tag="OHO85_004195"
     CDS             1880..2461
                     /locus_tag="OHO85_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HCQ2673480.1"
                     /translation="MLPSLNHIILTVALQALREGNIHHCETMGFTYDEMNLLGCLSIN
                     DLITLSQAPLPLVDITIRHDVLQKLLASSHEENRRQEQLNRAVRLGGSIALMNRYFGV
                     GSRETCARRRLLGVSVPNGRTPIPDEETDAAIWHQWQKYRVENIDSPEALDAMMQVTE
                     NLSSCTAPSLTVVWNCITHCERKNTVRRIQRAR"
     gene            2451..2660
                     /locus_tag="OHO85_004196"
     CDS             2451..2660
                     /locus_tag="OHO85_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HCQ2673481.1"
                     /translation="MPDEIDRDQAFNERCLEALIEQSRLRPTPTPSLQHCRFCGKAIP
                     EKRRQTLPGVTTCTDCQSILEKRRR"
     gene            2864..3031
                     /locus_tag="OHO85_004197"
     CDS             2864..3031
                     /locus_tag="OHO85_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673482.1"
                     /translation="MNDGSLLEKVVGHMEEKLTGNELEMLYAAADYRRAEIVMNQYFD
                     KVPGNVWKYVR"
     gene            3600..3833
                     /locus_tag="OHO85_004198"
     CDS             3600..3833
                     /locus_tag="OHO85_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673483.1"
                     /translation="MRKPVYYAYVAQESHPDSQGEKNTWWTKVGVAFPHHGKSGLNIV
                     LTPGIAVSGKLVLLEPREENNPPQNTGPIETVS"
     gene            3906..4616
                     /locus_tag="OHO85_004199"
     CDS             3906..4616
                     /locus_tag="OHO85_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCQ2673484.1"
                     /translation="MLSTTAFLAIAMQCAASIHPSTAFDVARVESGFNPYAIAEIVPK
                     AGPSFGDQHIISHLPASRGDAEHILRRLSAQDRRYSVGLMQITSTNFRHYGVSATELL
                     DPCTNLSVFEHILRDCYRRGGTLKRALSCYYSGNFSTGQQPETAFSGTSYIQRIGYPP
                     GSSRYAVPGTREDKSSVAVPLKTAPASDSSRHHVIWPGVVVRGVPAKLRQEKIFIAPF
                     SAPSVRRSFSLSPREEEE"
     gene            4616..4909
                     /locus_tag="OHO85_004200"
     CDS             4616..4909
                     /locus_tag="OHO85_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012885319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HCQ2673485.1"
                     /translation="MILFRKLKAVVPPVSACTLTTFSATPALADGFQRANTVMQKVST
                     GLHGLAAITITVAVIWIGYKTLWKGESLSQCGYIIIGGILIGGGSEIGALLMS"
     gene            4922..7660
                     /locus_tag="OHO85_004201"
     CDS             4922..7660
                     /locus_tag="OHO85_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HCQ2673486.1"
                     /translation="MAKLLKAMKRPAALWGVPMVPLLAVTGVTIIVAIWTSVALLFLL
                     PVQFLVMKSLTRNEPMRFNLIAVWLRAKGKPVANRLFGATTFMPVEHDDVDIKEFLDA
                     MKLNQCATIKKYIPYSSHIHQHVVRSPKSDLYCTWELMGTPFDCESDESLQFGTNQLH
                     GLIRSFEGMPVTFYIHNDRNTFTDNLHKDSGNPYADEVSRLYYASVGLFRRNRLFLTV
                     CYRPFVSLEKAERKRMKDSQKLKELDGALLEMLEIKSTLDTALSRYGARPLGTFTEGN
                     AVFSSQLAFYEYLLTHQWRKVRVTRTPAYDVMGAAALFFSAESGQINHANGTQYFRGL
                     EVKEFSEETATGMMDSLLYAPCDYVITQSYTCMSREEAKKAIKRTRRLLMSADDDAVS
                     QRLDLDVALDLLTSGKIAYGKHHFSIMVYSPSLESLVADTNEISNALNNIGITPVPAE
                     ISLSAAYMAQLPGNYNLRPRKGELSSQNFVELAALHNFYPGKRDKAPWGDAMALLRTP
                     SGDGYYINLHNTLADKDEFNEKNPASTCILGTNGSGKTMLMTFFEIMQQKYGREDSFS
                     PDAKTKRLTTVYLDKDRGAEMNIRALGGRYYRVISGESTGWNPFSLPATKRNINFIKQ
                     LMKILCTRNGSTISPRDERRLSDAVNAVMNDEPQYRVYGITRMLENLPEPATKEAQEN
                     GLSIRLSQWAQGGEFGWVFDNESDTFDISNCDNFGIDGTEFLDDASVCAPISFYLLYR
                     ITSLLDGRRLVIFMDEFWKWLRDPVFKDFAYNKLKTIRKLNGMLVVGTQSPAEIIKDD
                     IAPAVIEQCGTQILAANPNADRAHYVDGMKFEPEVFDVVKGLDPQARQYVVVKNQFKR
                     GDTKRFAARVTLDLSGIGRYTKVMSGDAPNLEIFESIYREGMQPHEWLDTYLAKAL"
     gene            7678..8385
                     /locus_tag="OHO85_004202"
     CDS             7678..8385
                     /locus_tag="OHO85_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HCQ2673487.1"
                     /translation="MKTRFRTLILACVIASPLAHAGIPVAVDADPMRDVQWAQELKRW
                     METARHYQSQIQAYKDQLATATGVRDIADFVDQAKGLKADLEKLRKPGQALNDLLLSG
                     GSSGQFDALYEKYKIFDTCNTAQSGSYANVCKQQVINKAIQLEQTDEVQNQVSQTLGE
                     INSLSNRVALAKDSKESQDLANSIQLKSVMLNTLTTQWEMSVKAAEKRENALEAERVK
                     QWNQQQLNAPTADLNNL"
     gene            8393..8635
                     /locus_tag="OHO85_004203"
                     /pseudo
     CDS             8393..8635
                     /locus_tag="OHO85_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
     gene            8639..9712
                     /locus_tag="OHO85_004204"
     CDS             8639..9712
                     /locus_tag="OHO85_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HCQ2673488.1"
                     /translation="MSGGLFVGVEKYLIGGITDATKGLMMSYSSMMMGLAAASATIYI
                     MWRGYQTLAGKLSTPMEDTMWDIMRMAIILSFVANLGGYLDGVIDAINGIKEGFSGSD
                     NIWQLLDTLWNKAKVLGKTLHDMDNSTYVKDEGMTAQFCVWLGIFALMILAGFVSMVA
                     EVMILLLGITAPIFIFCLMYGFLRPMFNNWLQNIFAGILTILFAALSLRIVVNYLNLV
                     LNKATKVADSSTIVELGAQVCLAGIFAAILVYISAKVAAALAGASATVSMQGLAAVGI
                     GAAAFGAGKLAGGAVKETKRSAQDNIQGWKDANAGKPGKPGGELGYNASKARKYAIEQ
                     MIARNEARRLAQSRESAVKKFNS"
BASE COUNT         2571 a         2423 c         2418 g         2314 t
ORIGIN      
        1 agcttgccgg gtgcttcttc cagccggcga ttctgtgctg cacgcaccac gaagcgctgc
       61 ccgtggctga ctttataatg caggtaatgc cagatatccg cttcccggtc acagacagtg
      121 attacccgtt tctgtatctc ccccagccgt tcggccatgc gctccgaagc ctgctgccag
      181 cggtaacttt ctttttcttc atagggacgt tcttttcgct ggtgcttaac accgtaggtg
      241 tccgtggcac gactccagcg ctgctgttcg ataagaccga ctggcagggc gctgtcgggg
      301 gcgtacatca ggacagagtg agccagcagc ccgcgcgtct tcgggttagt ggtggtattc
      361 cccaggtcat cagatgccgt actgtgactg aagttaatgg tggtggtgtc ttccagcgcg
      421 aggagcagcg gatgagcctc acatgccctt acagtggcgg taaatccggc ttcggcaatg
      481 gcttgcgggg acacagacgg gttacgtatc aggcggtacg caccttcaac ctgagcagtg
      541 gactgggatg atttcacaat agaaagacct gcatgctgag cgagagaaga ggccagtgac
      601 acaaggcgtc gtgtacgacg cggatcaccg agacgggcat gtccaaactg ctcgttagcc
      661 catgaataac aatcagaaag taccataaca gacccgaaga aaatgaaata taagagaaga
      721 tcaacgggtg aggaaaaagt tcaaaaaatc gctgccgggg aggaaggaaa ctaccggatg
      781 gaaagagtcc ccctaaagca gactgacaga catcacaaat ccccgggtgg ggatttgtgt
      841 ataagagaca gccccaattg tgggggcaag aaaaactaac catggtatta tagtaagatt
      901 ttcagaggag ctttttcaaa ttttcgaaac gatgagttgg aaagaacgcg tagaaaaaca
      961 aatttccaga ctacctaaaa acacagcatt agatgtcata aaaaaactaa cagaagtgac
     1021 gaccataaaa tataatcaca atgggtgctt tcctctttat acattaccgc ctgatgcctg
     1081 ttttgttatt cgccacaccg aagtcgaaag actaattaat ttatacaaaa aaagggagag
     1141 tcatcccatt tcaccttccc gtatgacaac accgttatca cgcctgtttt ggctggcctg
     1201 caagcacaat gatactatca gcccactgct gaatcatccc tataagctcc tttctatttt
     1261 tgaacaatgg gcatcagacg atggcatcgg ggaaaaactg gacgctgaaa cactgaaaaa
     1321 tgcgctgaag cgtggttcgc catcttcgac atccttatcc ggctaatacc atccccattt
     1381 gtaatggccg acaaccctaa ataagggttt tgcatccctt atttagggct caccagcttt
     1441 ctctcaggct tccactgtgt ttttttattc tccggccacc gtttatttca gtcacaggag
     1501 gatatatgac atcttatcag ttactacgcc tcagacaggt tgagcaaaaa acagggctga
     1561 agcgttcaca gatatacctt tatatgaaag agggtgcatt tccccgttca ataaaaatag
     1621 gccctgctag tgttgcctgg ctcgagtctg agattgacga gtggatcaac aaaaagttat
     1681 ctgaccgata aagacgcaat gaggagaaga tgatgaacat taatatcccg gcactcataa
     1741 acttcgatcc tgcaggtata aaaaaaagca cctgcatgta tatttcagac ctgcccggta
     1801 ttgtgctata tgacactata ccattcactg aatattcagc tgaattactg agcacttatt
     1861 cagttaagga gtcaatttca tgcttccatc cctgaatcat attatattaa ccgttgctct
     1921 tcaggcgcta cgggaaggaa atattcatca ctgcgaaact atgggattca cttacgatga
     1981 aatgaatttg ctcggttgct tatcaataaa cgatcttatc accctcagtc aggcaccttt
     2041 accgcttgtc gatattacta tccgccatga tgtcctgcaa aaattactgg catcatccca
     2101 cgaggaaaac aggcgtcagg aacaactgaa cagagctgtc aggctggggg gctcaatcgc
     2161 actgatgaac cgttatttcg gggttggctc cagagaaacc tgtgcacgcc gccggttact
     2221 cggtgtctcc gttccaaacg ggagaacacc cattccggat gaagaaaccg atgctgcaat
     2281 ctggcatcag tggcaaaaat atcgggtcga aaacattgat tccccggagg cgctggacgc
     2341 catgatgcag gttacagaaa acctttcatc gtgtactgcc ccctcgctga ccgttgtctg
     2401 gaactgcatc acgcattgcg aaagaaagaa cacggtaagg agaatacaac gtgccagatg
     2461 aaatcgatcg cgatcaggcg tttaatgaac ggtgcctaga agcactgatt gaacaaagcc
     2521 ggttaagacc cacaccgaca ccttccctgc agcactgtcg attttgcggc aaggctattc
     2581 cggaaaaacg ccgtcagacg ctgccgggtg taacaacctg tactgactgt cagtcgatac
     2641 tggagaaacg cagacgttaa atctgtatgt gcggaggaga atagctccgc acattttaat
     2701 atgcaccagc cggcttgttt tcaggttccc gtgcctccat gtcatggatg aataacctgc
     2761 tcacatctcc tgctgtggca atcctttttt acagtcagta caccagaggt cagacatgaa
     2821 caaaccggca aacaagatgg aattcgtcag aaaactgcgg taaataaatg acggcagcct
     2881 gctggaaaag gttgtcgggc atatggaaga aaagctcaca gggaatgagc ttgaaatgct
     2941 ttatgccgca gcagattatc gccgcgcaga gattgtaatg aaccagtact ttgataaggt
     3001 tcccggtaat gtctggaaat atgtcagata actctgtgtt ttttatgcaa caggtacaga
     3061 cggacaccac tgcggcatga aaacgaattt caccacccgg cccggtaagt atcggcctaa
     3121 gtggccggga aacgtttaac cagattaaag accgtccctt acaacaaaag ggactactgt
     3181 attcctgtct ttcacgcctt acacattatt gtcgttacca aattcagcca cagagacagc
     3241 atacaacccc atctgacata taaagaaagt cttcctccgg atgtttaatt taacctgata
     3301 ataatcaaca aatattatta tcagcccgcc cgtcatcatt catgcacaca cacaccgtgc
     3361 cagtaatacg ttaatttcac atactttttt atcattcctt ccggtattta ttttcccgtt
     3421 aaaatttcat tacccctcct gcatcctgac catgctcttt aacaatatgg agcgggctgt
     3481 cacacaacca taattcaggg tgccgctgaa tgttctcgct gacccgttaa aaccggaatg
     3541 caggatcgga atgtgatcga agaaatcatc ttcaacaatt ttaattaaac gaggacatta
     3601 tgagaaagcc tgtttattac gcgtatgttg cccaggaaag tcatccggac tctcagggtg
     3661 agaaaaatac ctggtggaca aaggtcggag tcgcctttcc ccaccacggt aaatccggtc
     3721 tgaatattgt cctgacgccc ggtattgccg tttccggcaa actggtcctg cttgagcccc
     3781 gggaggaaaa taatccacca cagaatacgg gccccatcga aaccgtgtcg taaccaccct
     3841 taattctgtt cccacagcat gcagtatcag cagaccctgc atgtcacttg tttactgagg
     3901 tcaccatgct ttccaccaca gcctttctgg cgatagccat gcagtgcgcc gccagcattc
     3961 atccgtccac ggcattcgac gtggcacggg tggaatccgg ttttaaccct tatgccatcg
     4021 ctgagattgt cccgaaggcc ggcccctctt tcggcgatca acacattatt tctcacctgc
     4081 cagccagccg gggagatgca gagcatattc tccgtcgcct gtcagcacag gatcgccgtt
     4141 actctgtcgg gctgatgcaa atcaccagca ccaatttccg ccattatggt gtgtccgcca
     4201 cagagctact tgatccctgt accaatctgt ccgtcttcga acacattctc agagactgct
     4261 accggcgcgg cggcacgctg aaaagggcgc tgagttgtta ttattccggc aatttcagta
     4321 ccgggcaaca acctgaaacg gcgttttccg gaacgagcta tatccagcga atcggttatc
     4381 cgccgggatc atcccgctat gcggtccccg gaacccgtga ggacaaatca tcagttgccg
     4441 ttcccctgaa aaccgcccct gccagtgact catcccgtca ccacgtgatc tggcccggag
     4501 tcgtcgtccg tggcgtccct gcgaaactcc gccaagaaaa aatcttcatc gcaccgttct
     4561 ccgcgccgtc agtgcgccgg agtttctccc tgtcaccccg tgaggaggag gaataatgat
     4621 acttttccgc aagctaaaag ctgtggtccc ccccgtttcg gcctgtacgc tgacgacttt
     4681 ttctgcaacc ccggcactgg ccgacggatt tcagcgggca aatactgtca tgcaaaaagt
     4741 gtcaaccggg ctgcatggtc tggcggcaat cacgatcacc gtggccgtga tctggattgg
     4801 ctataagacc ctgtggaaag gcgagagcct gtcgcagtgc ggctacatca tcattggcgg
     4861 cattttaatt ggcggaggca gtgaaatcgg cgctctgctg atgagctaag aggctgaaca
     4921 tatggcaaag ctgctcaagg caatgaaacg cccggcggcg ctgtgggggg tgccaatggt
     4981 gcccctgctg gctgtcacgg gtgtaaccat catcgttgct atctggactt ccgtcgctct
     5041 cctgttctta cttcctgtgc aatttctggt gatgaagtcc cttaccagaa atgaaccgat
     5101 gcggttcaat ctgattgccg tatggctgag agcgaaggga aagccggtag caaaccgttt
     5161 atttggcgca acaacattta tgcccgttga gcatgatgat gttgatatca aggagtttct
     5221 cgacgccatg aaactaaatc agtgtgccac catcaagaag tacatcccgt actcatcgca
     5281 cattcatcag catgtggttc gttcccctaa gagcgatctg tactgcacct gggaattgat
     5341 gggaacgccg ttcgactgcg agtctgacga gtcgctccag ttcgggacga accagttaca
     5401 cgggctaatc cgctcattcg aaggtatgcc agttactttc tatatccata acgaccgcaa
     5461 tacgtttacc gacaatcttc ataaagattc cggcaacccg tatgccgatg aagttagccg
     5521 tctttactac gcttctgtcg gtctgttccg gcgcaaccgc ctgttcctga cggtctgcta
     5581 tcgcccgttt gtcagccttg aaaaagcaga acgcaaacgg atgaaagaca gccagaaact
     5641 aaaggagctg gacggtgctt tgctggaaat gctggaaata aaaagcacgc tcgataccgc
     5701 gctgtcgcgc tatggtgcgc gacctctcgg tacgtttacc gaaggaaacg cggtgttttc
     5761 gtcgcaactg gctttctatg aatatctgct aacgcaccag tggcgaaaag tgcgcgtcac
     5821 ccgtaccccg gcctatgacg tgatgggggc ggcggcgttg ttcttttcgg ctgaatccgg
     5881 gcagataaac cacgccaacg gtacgcaata tttccggggg ctggaggtta aagagttctc
     5941 cgaagagacc gccacgggga tgatggactc ccttctttac gccccctgcg attacgtgat
     6001 cacgcagtcc tatacctgca tgtcacgcga ggaagcgaag aaagccatta agcgtacccg
     6061 tcgccttttg atgagtgccg acgacgatgc tgtttcccag cggctggatc tggatgtggc
     6121 gcttgacctg ctgacatcgg ggaaaatcgc ttacggcaag caccattttt ccatcatggt
     6181 gtattccccg tcgctggaaa gcctggttgc tgatacgaat gaaatcagta acgcgctgaa
     6241 caacataggg ataacgcctg ttccggcaga aatatcgctt tcagcggcgt atatggcgca
     6301 actgccaggc aactacaacc tgaggccacg taaaggggag ctgagtagcc agaactttgt
     6361 ggagctggcc gcgctccata atttctatcc gggcaagcgc gataaagccc cctgggggga
     6421 tgcgatggct ttactgcgca ccccgtcagg cgatggctac tatatcaacc tgcataacac
     6481 actggcagat aaggacgaat tcaacgagaa gaacccggcc agtacctgca tcctcggtac
     6541 gaacggttcg ggtaaaacca tgttgatgac gttctttgag atcatgcagc agaaatacgg
     6601 gcgcgaggat agcttctccc cggatgccaa aaccaaacgg ctgacaaccg tttacctcga
     6661 taaagaccgg ggcgcagaga tgaacatccg ggcgctgggt ggccgttact atcgggttat
     6721 cagtggtgag tctacagggt ggaacccgtt ctcgttgcca gcaaccaaac gcaatatcaa
     6781 tttcatcaag caactgatga aaatcctttg tacccgtaac ggctcgacga tctccccacg
     6841 cgatgaacga cgtctgagtg atgcggtcaa tgctgtgatg aatgacgagc cgcaatatcg
     6901 cgtctacggc attacccgaa tgctggaaaa tttgcctgag ccggctacga aagaagccca
     6961 ggaaaacggc cttagcattc ggttgtcaca gtgggcgcag ggcggtgagt tcggctgggt
     7021 gtttgataac gagtctgaca cgttcgatat cagcaactgc gataacttcg gcatcgacgg
     7081 aacggagttc ctggacgatg caagcgtatg tgcgccaatc tcgttttatc tgctgtaccg
     7141 cattaccagc ctgcttgacg ggcgacgcct ggtcattttc atggatgaat tctggaagtg
     7201 gttacgcgac ccggtgttta aggactttgc ttataacaag ctgaaaacca tccgtaagct
     7261 gaacgggatg ctggtcgtcg gtactcaatc cccggcagag atcatcaaag atgatatcgc
     7321 cccggcagtg attgagcagt gcgggacaca gattctggcg gcgaacccca acgccgaccg
     7381 cgcacactat gttgacggta tgaagtttga accggaagtg ttcgacgtgg tgaaagggct
     7441 tgacccgcaa gcccgccagt atgttgtggt gaaaaatcag ttcaagcgcg gcgataccaa
     7501 acgttttgcc gctcgtgtca cgctcgacct gtcgggtatc ggcagataca ccaaagtcat
     7561 gagcggcgat gcccccaacc tcgaaatatt cgaatcgatt taccgggaag gaatgcagcc
     7621 tcatgaatgg ctcgatacct acctggctaa agcgctctga cccaaaggag atatacgatg
     7681 aaaactcgct ttcgtactct tattctggct tgcgtcattg caagcccact ggctcatgct
     7741 ggtattcccg tcgctgttga tgctgaccct atgcgggacg tgcagtgggc gcaggagtta
     7801 aaacgctgga tggaaacggc cagacactac cagtcacaaa tacaggcata taaagaccaa
     7861 ctggcgacgg ccactggcgt tcgtgatatt gctgattttg ttgaccaggc caaaggcctg
     7921 aaagctgacc ttgaaaagct gcgtaagccc ggacaggcac tgaatgacct gcttctctcc
     7981 ggcggttcct ccgggcagtt cgatgcgttg tacgagaagt ataaaatttt cgacacctgc
     8041 aacactgcgc aatcggggag ttatgccaac gtatgcaagc agcaggtcat caacaaggct
     8101 atccagcttg agcaaaccga cgaggttcag aaccaggtaa gccagaccct gggggaaata
     8161 aacagtctct ccaacagggt agcgttagcc aaagattcga aagaatctca agaccttgca
     8221 aacagtattc agctaaaatc tgtcatgctg aacacgctta ccacccaatg ggaaatgagc
     8281 gtgaaagcag cagagaagcg cgaaaatgcg ctagaagcgg agcgtgttaa gcagtggaat
     8341 cagcagcagt taaacgcgcc aactgctgac cttaataatt tatgaggtat ttatgttatc
     8401 aaaaaaaatt attttaattt cgacgatcac cctgtgcttt tctattgttg gatgcaaaga
     8461 ggaagctaaa acaacaaaat ggtataggga tcaccctgac taattaaaac tagtttatga
     8521 aaagtgccaa aagagtggtg acgcttctga aaattgcaag aatgcaaatg aagcccacta
     8581 tcaaatcaag cagctaaacg caccaactcc agacttaaat aatttagagg agtaatatat
     8641 gagtggcggt ctttttgttg gggttgaaaa atacctgata ggaggaatta cagacgccac
     8701 aaagggattg atgatgtcgt actcttctat gatgatgggg ctggcagcag cgtcagctac
     8761 catctacatc atgtggcgtg gataccagac tttggctggt aagctatcta cgcctatgga
     8821 agacacgatg tgggacatca tgaggatggc gatcatcctg tcctttgtgg ctaatttagg
     8881 cggctacctc gatggcgtta ttgatgccat aaacggaata aaggagggtt tttctggtag
     8941 tgataatata tggcagttat tagacaccct atggaataag gctaaagtgt tagggaaaac
     9001 gctacatgac atggataatt ctacatatgt aaaagatgag ggaatgactg ctcaattttg
     9061 tgtatggctt ggaatattcg cacttatgat tctagcgggc tttgtttcaa tggttgccga
     9121 agtaatgatt ctactattag gaataactgc acctattttc atattctgcc ttatgtatgg
     9181 tttcctccgc cccatgttca ataactggtt gcaaaatata tttgcaggaa tattaacaat
     9241 tctatttgcc gctctctcat tgagaattgt agtgaattac ttgaatctcg ttcttaataa
     9301 ggcgacgaag gtagcagaca gttcaacaat tgttgaactt ggtgctcaag tctgccttgc
     9361 aggtattttc gctgcaatac tggtgtatat ttccgctaag gtagccgcag ctctggccgg
     9421 ggcaagcgct accgtatcca tgcaggggct ggccgctgta ggtatcggtg cggctgcttt
     9481 tggtgccggg aagctagccg ggggtgcagt taaagaaacc aaacgttccg cccaggacaa
     9541 cattcagggc tggaaggatg cgaatgcagg taagcccggc aagcccggag gtgagttagg
     9601 ctataacgca tctaaagcgc ggaagtacgc gattgagcag atgatagccc ggaacgaagc
     9661 gcggaggctg gcacagtcga gagaatccgc ggttaagaag ttcaacagct aggccagacg
     9721 tctccc
//
LOCUS       DAJOWB010000155         9495 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJOWB010000155 DAJOWB010000000
VERSION     DAJOWB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.155"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            85..1164
                     /locus_tag="OHO85_004205"
     CDS             85..1164
                     /locus_tag="OHO85_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012815875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HCQ2673489.1"
                     /translation="MKKRKRGKATAFLAIVAVTLIFLAWGGNWVYRNILWQPTEEKKQ
                     DTAPQTNKTDYRQRNDLGMSTDTAEEEPEQQDNGQSSTGGTGQVAPAAPPELNKASFL
                     IRRDGSATTQNPVKTRQQEMTLTSATTTGQQDSSAATNTAAPPPGQSPAPVRRIPYNP
                     DLYIPENTSIPCSLDRRFVSDRAGKLRCTVTTDIWSASGNTKLIEKGTTASLLYRAIA
                     EEGMKHGQGRAFIIATKLRTRQPPYLDIPLVDTSAAGDLGEAGVDGWIDSHFWERFGG
                     ALMVGMIPDIGAWASNSAGKKDRNTDYTENSRQAMADMARTTLENSINIPPTLYKNQG
                     EIINLITGEDIDFSNIYTLRLKNDR"
     gene            1154..2179
                     /gene="virB11"
                     /locus_tag="OHO85_004206"
     CDS             1154..2179
                     /gene="virB11"
                     /locus_tag="OHO85_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="HCQ2673490.1"
                     /translation="MTAKNISLDRYKQRFFGDFLELPGLTEIAVNRPGELYTKINGVW
                     EQHAVPLSYEDCYNFARCLAKHHGDNIEDIKPGLSATLESGERCQVIVPPACERDTVS
                     ITIRKPSKVQIPHQSYIDAGFYNRVTGEEKTETHDEELIALYNTKNIPLFMEKCVEYG
                     KTLAVAGETSTGKTTYMKMLIGYIPVHLRISTIEDNPEITFFIHKNYVHLFYPSESSD
                     EKGSIVTPASLLRWNYRMNPDRILLTEVRGAEAWDFLKITGSGHEGSMTSIHAGSAKE
                     AIDGFITRCYENPQCAQLPYTFMLRKVLDSLDVIVSIDLDGNVRRMNDIYFRPIHRNQ
                     YFEEMKA"
     gene            2176..2574
                     /locus_tag="OHO85_004207"
     CDS             2176..2574
                     /locus_tag="OHO85_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="HCQ2673491.1"
                     /translation="MIKKLKVIMLAAIVTGCSSPPPPVPVEWDKAADPLNTSLPQWSD
                     NNVIVPSPTVNGKWTSSISAQSFYDTIWTPAVFYAVAHSTRIVVTAQSGTDFFNAKNW
                     LRQNGAKGVIEYQPVFNCLTCRETTIYFSR"
     gene            2610..2915
                     /locus_tag="OHO85_004208"
     CDS             2610..2915
                     /locus_tag="OHO85_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCQ2673492.1"
                     /translation="MKKTVIAATVLCLTAGTPIYSFAADPCEVVLCMYGKATGNSGGS
                     ECSSAERAFFKINAFKKHHRFNPGKTFDMRKEFLGGCKAADPESISKILSKFGRVKG"
     gene            2949..3254
                     /locus_tag="OHO85_004209"
     CDS             2949..3254
                     /locus_tag="OHO85_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011172016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dpoa decarboxylase"
                     /protein_id="HCQ2673493.1"
                     /translation="MINEDLFIKNIHSKNQDRISVAMVYDTLSKEAHRGCGLHYEIYE
                     SCFIGLLRDHLSELNEADANRLRRYAESKGTKIDDASYSEALEAERECRAEIYREQM"
     gene            3964..5823
                     /locus_tag="OHO85_004210"
     CDS             3964..5823
                     /locus_tag="OHO85_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HCQ2673494.1"
                     /translation="MILNKLTASLSPIVNGMLALLAFVQQQQLILALLAGLTMPFFTS
                     MKSDERQKAPLWKKLIIAFSLLCFISGTMAPIVIGSFQWLYKIRLTSDNTVLAWAVRI
                     TFTVTGIIFHIMLRRVFTPELDRIKKRLVKKTTLERELRTDVRTVKSLLPETLHYNPL
                     DYIDLNKGIFTGMDSEKEPLYLPLKDWQKQHADIIGTTGAGKGVAAGILLYQSILAGE
                     GVFVMDPKDDEWAPHLYRKACEDAGKPFALIDLRKQQYQLNLIEDITPDELEELFVAG
                     FSLAEKGQESDFYRIDDRKAARMAAQFVSDNPSATLRDVYNGDYVQSIAEKIKAFFGK
                     IEELALLNAINSPEGFSLQQMFDEGGCCYVIGSMRNSKIITTQRMLLVRLYQLAERRD
                     RVTNVPRSIAIYLSELKYHLSRPALEGLGAARDKGVHIIMDHQSIADLKDCPADLKGD
                     AVVGAVVENAKFKLVYRVMDPDTAEWVARMSGTILVDDELRKAKTDNMLTETIDSERT
                     IRQAERYFIDSNMILNLHDFVSFIFTTKTLPSASLISPIKIRKRQLKIFSVSPDIAAS
                     AAPVKIALDFDEDDKPSIQDVMTTHPDLFFNESEVQPAEPQPDEGEHLSPLDF"
     gene            5833..6579
                     /locus_tag="OHO85_004211"
     CDS             5833..6579
                     /locus_tag="OHO85_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HCQ2673495.1"
                     /translation="MLISTYQERQTRNREKIKQLLNFLKEETYSDFKTLMLLFGFRDH
                     KSLYSLLAKTEQMGLIQKHVLESRTMKISLWGITSDGLAMVLTPDDKIFPARFEPSRI
                     TGWTLEHHLDNQAARLILEKKGASGWINGDRTTFISKYQVKHRPDGLLTLPDGKVIAI
                     ETERRLKTRARYQSIIASHLLARTRKNWIYVFYVMPDPQKKRALELLFDSIRHVIINH
                     QHVPLEERHRRVFRIYTFDELKHLSLGSYT"
     gene            complement(6773..7717)
                     /locus_tag="OHO85_004212"
     CDS             complement(6773..7717)
                     /locus_tag="OHO85_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1738 domain-containing protein"
                     /protein_id="HCQ2673496.1"
                     /translation="MKISLHTQTRAPNAAATTSVSPLDPSGSSKTKFSKTRTDIYQTI
                     TDNIITALEAGVKPWSCPWQRVSGMSGLPSNYATGVPYSGMNIMLLWCSASKQGFSDS
                     RWMTYKQSQTAGGQVRKGEHGTTAIFYTTLEKENDAGEIDHIPMLKTFTVFNVQQIDG
                     LALTTETVSPEATFDPMPQAENLFRKSGASIIEKGQNAFFSPSSDEVWLPARHLFSDA
                     ANFYATGLHELVHWSGGKKRLNREMKGKFGSADYAEEELVAELGSAFLMAELGIEGEV
                     QHESYIASWLKALKNDKRYIFKAASAASKAHRYLMDKL"
     gene            8142..9350
                     /locus_tag="OHO85_004213"
                     /pseudo
     CDS             8142..9350
                     /locus_tag="OHO85_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012669257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2678 a         2197 c         2240 g         2380 t
ORIGIN      
        1 gaagcccgcg ctaaaaggga ggccgagctt ctcaatgcgc aggacgacga agagaaagac
       61 ccggtacaac ccgccgttaa caagctgaaa aagcgcaaac ggggcaaagc aaccgccttt
      121 cttgccattg tcgcggtcac gcttatcttc ctggcatggg gtggtaactg ggtttatcgc
      181 aatatcctgt ggcagcctac ggaagaaaag aaacaggaca cagccccgca gacgaacaaa
      241 accgattacc ggcagcgtaa cgatctcggt atgtccaccg atacagccga agaagagcca
      301 gagcaacagg ataacggcca gagcagcacg gggggaacag ggcaggtggc tccggccgcc
      361 ccgccagaac tcaacaaagc cagcttcctt attcgccgtg atggttcggc caccacacag
      421 aacccggtaa aaacccggca gcaggaaatg acgctgacat cggcaacgac caccggacaa
      481 caggacagca gcgcggccac aaataccgcc gcaccgccgc cgggacaaag ccccgcacca
      541 gtgcgccgca taccctataa ccctgacctg tacatcccgg aaaatacctc gatcccctgc
      601 tcactggatc gccgctttgt atctgaccgg gcggggaaac ttcgctgcac ggtcactact
      661 gacatctgga gcgccagcgg caacacgaag ctgatagaga aaggcaccac cgcatcatta
      721 ctttaccgcg ccatagcgga agaaggtatg aagcacgggc aagggcgggc attcatcatt
      781 gcgacgaagc tacgcacccg ccagccgcct tatctcgata tcccgttggt tgataccagc
      841 gcggctggcg atctgggcga ggctggcgtt gatggctgga tagattcgca cttctgggaa
      901 cggttcggcg gcgcgttaat ggtcggcatg atccccgata ttggcgcatg ggcatccaac
      961 agcgcgggta aaaaagaccg caatacggac tatacggaaa acagccgcca ggctatggca
     1021 gatatggcac gtaccacgct ggagaacagc atcaatattc ccccaacgct ctataagaat
     1081 cagggcgaga tcatcaacct aatcacaggc gaggatattg atttttccaa catctataca
     1141 ctgaggttga aaaatgaccg ctaagaatat ttccctcgac cgctataaac agcgtttctt
     1201 tggcgacttt ctagagcttc ccggcctgac ggagatcgcc gtcaatcgtc caggtgagct
     1261 gtataccaaa atcaacggcg tatgggagca gcacgccgtt cctttgtcct atgaggattg
     1321 ctacaacttt gcccggtgcc tggcaaagca ccacggcgac aatatcgaag atattaagcc
     1381 ggggctgtca gccacgctgg aatctggcga acggtgccag gtgattgttc ccccggcctg
     1441 tgagcgcgat acggtgtcta tcaccatccg taagccatcg aaggtacaaa tcccgcatca
     1501 gtcatacatc gatgcgggat tttataaccg ggtaacaggc gaggaaaaaa cggagacgca
     1561 cgacgaggaa ttaattgcat tgtacaatac aaaaaatatt cctctgttta tggagaaatg
     1621 cgtcgagtac ggtaaaactc tcgcggtcgc cggggaaaca agcaccggga aaaccaccta
     1681 tatgaaaatg ttgatagggt atattcctgt acatcttcgg atatcaacca ttgaggacaa
     1741 cccggaaatc acgttcttta tccataaaaa ctacgtccat cttttttatc cgtcagaatc
     1801 atcagatgaa aaaggttcta tcgttacccc ggcgagcctt ttacgctgga attaccgtat
     1861 gaaccctgac cgcattttat taacagaggt tcgcggtgcg gaagcctggg acttccttaa
     1921 aataacaggt tccggccatg aagggagtat gacctccatt catgcaggat cagcgaaaga
     1981 agctatagat gggtttatta cccgttgcta tgaaaatcca cagtgcgccc agctccccta
     2041 tacatttatg ttacgtaagg ttctggatag cctggacgtg attgtgagca ttgatttaga
     2101 cggaaatgtt cgccgtatga atgacatcta tttccgtcca attcatcgaa accagtattt
     2161 tgaggaaatg aaagcatgat aaagaaactt aaagtcatca tgttggcagc aatagttacc
     2221 ggatgttcat cgccgccgcc tcccgttcct gtggagtggg ataaagccgc tgacccatta
     2281 aataccagtt taccgcaatg gagtgacaat aacgttatag tcccttcccc gacagttaac
     2341 ggtaaatgga catcatcaat cagcgcacaa agtttctacg atacaatatg gactccagca
     2401 gtattttacg cagtcgccca ttcaacacga attgtcgtca ctgcacaatc cggcactgat
     2461 ttctttaatg ccaaaaactg gcttcgccag aatggcgcaa aaggcgttat tgaataccag
     2521 cctgtattta attgcctgac gtgcagagaa accactattt atttctctcg ctaatccatt
     2581 taagcaacac aatctaataa ggatttatca tgaaaaaaac cgttatcgcg gcgactgtat
     2641 tatgcctgac cgcaggaacg ccgatttatt cctttgccgc tgacccgtgc gaagttgttc
     2701 tttgcatgta cggaaaagct acaggtaata gtggtgggag tgaatgcagt tcagcagaac
     2761 gcgcattttt taaaatcaac gcatttaaaa aacaccatcg tttcaatccg ggtaaaacat
     2821 tcgatatgcg aaaagaattt ttaggggggt gcaaagccgc agaccctgaa tcaataagta
     2881 aaatcctttc aaaatttggc agagtgaaag gataactgac atatccattt tgatatgaga
     2941 ggttcgaaat gataaacgaa gatttattca ttaagaatat acactcaaaa aatcaggaca
     3001 gaatatctgt agccatggta tatgacacgt tatcaaaaga agcccacaga ggttgtgggc
     3061 tgcattacga aatatacgaa agctgtttta tcggtctttt gcgcgatcat ctttcagaac
     3121 ttaatgaagc tgacgcaaat agattaagac gctatgccga gagtaaaggt acgaaaattg
     3181 atgatgcgtc ctacagtgaa gcactggaag ctgaaaggga gtgcagggct gaaatatacc
     3241 gcgagcaaat gtaatcacac aaaacaaaag tgtattgtac aatacagaga ctaacaggcg
     3301 acgggatttt aaggcttcat tatttttggc ttcaatttcg aagaaattgt taagacgaaa
     3361 aataatgaag tggaacgtgg acagaaaatt caatttcgga gaaattgcta attttcagga
     3421 tccacgtcca cccgcgaaca ttcgccccag cgaattgaag cgggccgggg ggagccgcaa
     3481 aaattttgcc gccccagcga gcaaaaaggc acgcccacgt gccggttttt gtggggggct
     3541 tgccccgcac accgtcgcag ccaggctata tttcccgccc cggcggggaa tatgccgatt
     3601 aggcgcgacc aaccccttta aagcagcgtt cccatttttt cgagcttgcg aagaaaaaat
     3661 aggctaaacg cgcgtcttaa aggggttggt cgcgcgtagc gggcgacggt gtgctgcctt
     3721 tgaccctggg ggttttggcg cggcgtccag ccacgacatt gcccccatgt gacaggcaat
     3781 aagggcgtcc agcccgcatg tactggtgca cgccgtttcg tgcagggaag catgctttcc
     3841 tgcacatggc cgcacagcga ccaccttctt taccccatac gttcgccccg ccttttcgcc
     3901 cgacaccgtg gggcgaactg atggggcgat gtcatggggc gaatcgctga cgaaggaggc
     3961 acgatgattt taaataagct taccgcttca ctgtcgccga tagtgaacgg gatgctggca
     4021 ctgctcgctt ttgtgcaaca gcaacaactg atactggcgt tattagcggg gctgacgatg
     4081 ccgtttttta cgtcgatgaa aagcgatgaa cggcagaagg ccccgttatg gaaaaagctg
     4141 attattgctt tttcgctgtt gtgttttata tctggcacaa tggccccgat agttatcgga
     4201 tcctttcagt ggctgtataa aatcagactc accagtgata atacggtact ggcgtgggca
     4261 gtacggatca ctttcactgt gaccgggatt atttttcata ttatgcttcg ccgggtattc
     4321 acgcctgaac tggacagaat aaagaaacgc cttgttaaaa aaaccaccct tgaacgggaa
     4381 ttgcgcaccg atgtccgcac cgtaaaatcc ctgctgccgg aaacactgca ttataacccg
     4441 ctggattata tcgacctgaa taaaggtatt tttactggta tggacagcga gaaggagccg
     4501 ttgtatctcc cgttaaagga ctggcagaaa caacacgcgg atattatcgg caccacagga
     4561 gccggtaaag gggttgctgc tggtatattg ctttatcaaa gcattctggc cggtgaaggt
     4621 gtgtttgtta tggaccccaa ggatgatgaa tgggcaccgc atctttaccg caaagcctgc
     4681 gaggatgcag gcaaaccctt tgccttaatc gacctgcgaa aacagcaata ccagttaaat
     4741 ctgattgaag atatcacgcc cgatgaactg gaagaattgt ttgttgccgg tttcagcctg
     4801 gcggaaaaag ggcaggaatc ggatttttat cgcattgatg accgaaaagc cgcacgtatg
     4861 gccgcgcaat ttgtcagtga taacccatcg gcaactctcc gtgatgttta taacggtgac
     4921 tacgttcaga gcattgccga aaaaatcaaa gcatttttcg gtaagattga agagctggca
     4981 ttattaaatg ccattaattc accggaaggt ttttcacttc aacaaatgtt tgatgaaggc
     5041 ggctgttgct atgttatcgg ctccatgcga aacagcaaaa ttattacaac ccagcgcatg
     5101 ttactggttc gcctgtacca gttagcagaa aggcgcgacc gtgtaacaaa cgttccccgc
     5161 tccatagcta tttatctttc cgaactgaaa tatcatttat cccggccagc gctggaaggt
     5221 ttaggtgcgg cgcgggataa aggcgtgcat attattatgg accaccagtc cattgccgat
     5281 ttaaaagact gcccggcaga tttgaaaggt gatgccgttg tcggtgcggt cgttgaaaat
     5341 gccaaattca aactggttta tcgcgtcatg gacccggata cggcggaatg ggtggcaaga
     5401 atgtcaggca ctattctggt ggatgatgaa ctccgtaagg ctaaaacgga taatatgctg
     5461 acagagacca tcgacagtga gcgcacaatc cgccaggccg aacgttactt tattgacagt
     5521 aacatgatcc tgaacctgca cgattttgtc agctttatct ttacgacaaa aacgctacct
     5581 tctgcctcac tgatttcacc gataaagatc agaaagcgtc agctaaaaat cttctcggtt
     5641 tcccctgata ttgccgcatc ggcggcaccg gtgaaaatcg cactggattt tgacgaggat
     5701 gataaaccat ccatacagga tgtgatgaca acgcacccgg atcttttctt caacgaaagc
     5761 gaagttcaac cggcagagcc ccaacctgat gaaggagaac acctgtcccc gctggatttt
     5821 taaggagcgg ttatgctgat ttctacatat caagagcgcc agacacgcaa cagagaaaaa
     5881 ataaaacaac tgctgaactt cctgaaagaa gaaacctaca gcgatttcaa aacgctgatg
     5941 ctgctatttg gtttccggga tcataaatcg ctttattcgc tgttggcaaa aactgagcaa
     6001 atggggttaa tccagaagca tgtgcttgaa tcacggacaa tgaagatttc attatggggg
     6061 atcaccagcg acgggctggc gatggtgtta actccggatg ataaaatctt tcccgcgagg
     6121 tttgaaccgt ccagaattac cggctggacg ctggagcatc atcttgataa tcaggccgcc
     6181 agacttattc tggagaaaaa aggtgcttcg ggatggatta acggcgaccg gaccactttt
     6241 atcagcaagt atcaggtaaa acatcgccca gatgggttac tgaccctgcc tgacggaaaa
     6301 gttattgcca ttgaaaccga gcgccgcctg aaaacccggg cccgttatca gtcgataatt
     6361 gccagtcacc tgctggcccg tacccgcaag aactggattt atgtttttta tgtcatgcct
     6421 gacccacaga aaaaacgggc acttgaactg ctgtttgaca gcatcagaca cgtcatcatc
     6481 aaccatcagc acgtcccgct ggaagaacgc caccgtcggg tgtttcgcat ttataccttt
     6541 gatgaactga aacacctttc attaggcagc tatacgtaag cttttattca ggtcatcgtg
     6601 atggcctcac cggcctggcg gcgataccgc caggccggaa ttttttttat tccactgcga
     6661 ggcgcagact gtccggtact cgctgtgctg cgttccgcct cagtctgcgc cctgcagcgt
     6721 tgccggttaa aaccatgtaa ccgcattccc ttgcggttgt ccggctccgg cctcagagtt
     6781 tatccatcag ataacgatgc gctttggagg cggcgctggc cgctttaaaa atataacgct
     6841 tgtcgttttt cagcgctttc agccaggaag caatatagct ttcatgttgt acctctccct
     6901 caatccccag ttccgccatc agaaaagcac ttcccagctc cgccaccaat tcttcctctg
     6961 cataatctgc actgccaaac ttccctttca tttcacggtt caggcgtttt ttaccaccac
     7021 tccagtgaac cagctcatgc aggccagtag cgtagaagtt agccgcatcc gaaaacagat
     7081 ggcgcgctgg taaccagact tcatcagagg atggactgaa aaaggcattt tgtcctttct
     7141 cgatgatgct tgcgccgctc ttcctgaaca gattttcagc ctgcggcatc gggtcaaagg
     7201 ttgcttccgg gctgactgtt tcagttgtca gggccaagcc gtcgatttgc tgaacgttaa
     7261 acacggtgaa agttttaagc atcgggatat ggtcaatttc tcctgcgtcg ttttccttct
     7321 ccagcgtggt gtagaaaatg gccgtcgtac catgttcgcc cttgcgaacc tgtccacctg
     7381 ctgtctgaga ctgtttgtag gtcatccagc gggaatcact gaaaccctgt tttgacgcac
     7441 tgcaccacag caacatgata ttcatgccgc tgtagggtac gccagtggca taattggatg
     7501 gcagaccaga catgcccgat acgcgctgcc acgggcagga ccacggtttt acaccggctt
     7561 ccagtgctgt gatgatgttg tcggtaatgg tctgataaat atctgttctg gttttagaaa
     7621 atttcgtttt tgaggagcct gacgggtcca gcggggatac gctggtcgtc gctgcggcgt
     7681 taggggctct agtctgggta tgcagggaaa tcttcatggt tttttctccg tcagtgtgtg
     7741 attttcgtct tcgtatcgcc tgacggttcg ctttcccggc atcgttccgg ccagcaaggg
     7801 cgcagcatgc gtgccattta cccttgcggg acgggttgtg ttgggaaaag attaaccgga
     7861 agcagatacg gagaacgaaa agcgcacgag agaaaaaact tgcccctgca tagccgggcg
     7921 tagcgcccct tgctatgcag cggtgaaaag ggtttgaact attgaatgaa taactgcacc
     7981 gtagcaatac gcatgcaatc aaaaccatca taatttattg atttaaaaga tttttcaaac
     8041 cactccttga gagtgaaaat tctgattgat tagtgaaaca attggttaga gaatgtaact
     8101 attcacacag aaatcctact gaaaaaaata aagagacagt aatgagtatg ttaatctgga
     8161 agaaaaaaaa taatgctaaa cacttaatct tgtttataca tggtttaaaa ggcggaagtg
     8221 atacgtgggc agttgataag atcacatcct tcccacagct tatatgtgag gatgaagatt
     8281 tttgtgatgc ctttgatata gcctgttttt agtatttcac taattttacg aatacctacg
     8341 gtaaaagtag aaatctactt gccaggttgt tcacatcact aacaaaaaaa gaagtcaatc
     8401 ttcctgttga tgaattatca gagttacttg ttggagaatg tcaaataaat ttatccgatt
     8461 atagtaatat aatatttgtc gctcacagca tgggaggttt aatagcaaaa tcatgcatta
     8521 ttaaaatgca tgaaagagat ctttctcaca atataactgg atttatttca cttgctgttc
     8581 ctcattcggg gtcagaaact gcttcatggg ctagtatggt ttcatcaaat gtccagcttg
     8641 gtgatttaag tgtatttagt aaagaaacag attctctaaa ccgcagatgg gtaaaattgc
     8701 ctaaactccc tgagttaaag tttttgtatg gaagctacga tagtattgta gttaaagcca
     8761 gtgcaattcc tgttcaagtt tctgccaaag agtctatagc tgttcaggag gatcattcga
     8821 caatttgcaa accaaaggat agggattcta atgtttatct tatcgttaag aaactagctc
     8881 ttgaaataca taacaaaacg gaactaattt catcttcacc agaatttaaa gatgataagc
     8941 aatatgacaa tgaatttttt gtattgaaaa tgatagtcgc tgatgtacat agtgatatat
     9001 caaaacatgc taaggagtat tattataatg cagaattggc cagaaatatt ttcactagcg
     9061 atcgagatag agaaacctta agtgttttgt atagaaaaat aagggagata tatcaaacac
     9121 agtatcatga ctctatagca aatcataata cagcaaacca actcttagct gcaatacaca
     9181 aaaaaataga agatgaagat aaggttaaat tatcatctct tcttgataca cttgatagtg
     9241 tacataagaa aggaatgctg catcagcttg ctaataagct agatagagat ataatttggt
     9301 ccccagacac atctcttgac tctttggata gtttaagggg gcaaaaatga tttcattacc
     9361 ttatattcct cttgatgaag attttaattt gaatgttgct cttctgatta ttttaattca
     9421 tacgctaagc aagaatagaa atgatgttct attgttggat agaaataaaa ttcaaatatt
     9481 tttatatttg attaa
//
LOCUS       DAJOWB010000156         9218 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJOWB010000156 DAJOWB010000000
VERSION     DAJOWB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.156"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1445
                     /gene="malX"
                     /locus_tag="OHO85_004214"
     CDS             <1..1445
                     /gene="malX"
                     /locus_tag="OHO85_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCQ2673497.1"
                     /translation="IPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENK
                     GVAAFAGFVGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAV
                     IAGIIVWMLHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGIS
                     GLGHMINSAGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALT
                     IFQAQLNCPTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLI
                     SGLIACVVGGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDF
                     VVFGILHGLSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVA
                     GAPGKSGYNVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQ
                     LNQHNLQVVIGPQVQSVKDEMAGLMHTVQA"
     gene            1455..2627
                     /gene="malY"
                     /locus_tag="OHO85_004215"
     CDS             1455..2627
                     /gene="malY"
                     /locus_tag="OHO85_004215"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCQ2673498.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            2731..3732
                     /gene="add"
                     /locus_tag="OHO85_004216"
     CDS             2731..3732
                     /gene="add"
                     /locus_tag="OHO85_004216"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCQ2673499.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLAKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(3767..4807)
                     /locus_tag="OHO85_004217"
     CDS             complement(3767..4807)
                     /locus_tag="OHO85_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCQ2673500.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            5050..5175
                     /gene="blr"
                     /locus_tag="OHO85_004218"
     CDS             5050..5175
                     /gene="blr"
                     /locus_tag="OHO85_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCQ2673501.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            5448..5663
                     /gene="ydgT"
                     /locus_tag="OHO85_004219"
     CDS             5448..5663
                     /gene="ydgT"
                     /locus_tag="OHO85_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCQ2673502.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            5749..6189
                     /locus_tag="OHO85_004220"
     CDS             5749..6189
                     /locus_tag="OHO85_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCQ2673503.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            6266..6847
                     /gene="rsxA"
                     /locus_tag="OHO85_004221"
     CDS             6266..6847
                     /gene="rsxA"
                     /locus_tag="OHO85_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCQ2673504.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            6847..7425
                     /gene="rsxB"
                     /locus_tag="OHO85_004222"
     CDS             6847..7425
                     /gene="rsxB"
                     /locus_tag="OHO85_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCQ2673505.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            7418..>9218
                     /gene="rsxC"
                     /locus_tag="OHO85_004223"
     CDS             7418..>9218
                     /gene="rsxC"
                     /locus_tag="OHO85_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCQ2673506.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKA"
BASE COUNT         2100 a         2359 c         2390 g         2369 t
ORIGIN      
        1 taatcccggt cctgggcaac cccgtgttgc aggctatctt tacctggatg agtaagattg
       61 gctcgtttgc ttttagtttc ctgcctgtca tgttctgtat cgccatcccg ctgggcctgg
      121 cacgcgaaaa caaaggcgta gcggcattcg ctggcttcgt cggttatgcg gtaatgaacc
      181 tcgcggtaaa cttctggttg accaataaag gcattctgcc aaccacggat gccgcggttc
      241 tgaaagccaa taacatccag agcattcttg ggatccagtc gatcgatacc gggatcctcg
      301 gtgcggtgat cgccggtatt atcgtctgga tgctgcatga gcgtttccat aatatccgcc
      361 tgccggatgc gctggcattc ttcggcggta cgcgcttcgt accaattatc tcctcgctgg
      421 tgatgggcct tgtcggcctg gtgattccat tagtctggcc gattttcgcc atgggtatta
      481 gcggcttggg ccatatgata aacagcgcgg gtgatttcgg accgatgctg tttggtaccg
      541 gtgaacgtct gctgttgccg tttggtctgc atcacattct ggtggcatta attcgcttta
      601 ccgacgcagg cggcacgcag gaagtctgcg gtcaaaccgt cagcggcgca ctgaccatct
      661 tccaggcgca attgaattgc ccgaccactc acggtttttc tgaaagcgcc acgcgtttcc
      721 tttcgcaagg taaaatgcct gcgtttctcg gcggtctgcc aggtgcagcg ttagctatgt
      781 atcactgcgc gcgcccggaa aatcgccata aaattaaagg tctgctgatt tctggcctga
      841 tcgcctgcgt cgttggcggc actaccgaac cgctggaatt cctgttcctg ttcgtagcgc
      901 cagttctgta tgtcatccac gcgctgttaa ccggcctcgg cttcaccgtc atgtctgtgc
      961 tcggcgtcac catcggtaat accgacggca atatcatcga cttcgtggtg ttcggtattt
     1021 tgcatggtct gtcaaccaag tggtacatgg tgccagtggt ggcggcaatc tggtttgtcg
     1081 tttactacgt catcttccgt ttcgctatca cccgcttcaa tctgaaaacc ccggggcgcg
     1141 atagcgaagt tgccagctca atcgaaaaag ccgttgccgg tgcgccgggt aaatcaggtt
     1201 acaacgttcc tgcaatcctc gaagcattag gcggtgccga caatattgtc agcctcgata
     1261 actgcattac ccgtctgcgt ttgtctgtga aagatatgtc gcttgttaat gtgcaggcac
     1321 tgaaggacaa tcgggcaatt ggcgtagtac aacttaatca acataacctg caggttgtta
     1381 tcgggccaca agttcagtca gtaaaagatg aaatggccgg tctgatgcat actgtccagg
     1441 cataaggata cgatatgttc gatttttcaa aggtcgtgga tcgtcatggc acatggtgca
     1501 cgcagtggga ttatgtcgct gaccgtttcg gcactgctga cctgttaccg ttcacgattt
     1561 cagacatgga ttttgccact gccccctgca ttatcgaggc gctgaatcag cgcctgatgc
     1621 acggcgtatt tggctacagc cgctggaaaa acgatgagtt tctcgcggct attgcccact
     1681 ggttttccac ccagcattac accgcaattg atacgcagac ggtggtgtat ggcccttctg
     1741 tcatctatat ggtttcagaa ctgattcgtc agtggtctga aacaggtgaa ggcgtggtga
     1801 tccacacgcc cgcctatgac gcattttaca aggccattga aggtaaccag cgcacagtaa
     1861 tgcccgttgc tttagagaag caggctgacg gctggttttg cgatatgggc aagctggaag
     1921 ccgtgttggc gaaaccagaa tgtaaaatta tgctcctgtg tagcccacag aatcctaccg
     1981 gtaaagtgtg gacgtgcgat gagctggaga tcatggctga cctatgcgag cgtcatggtg
     2041 tgcgggttat ttccgatgaa atccatatgg atatggtctg gggcgagcag ccgcatattc
     2101 cctggagtaa tgtggcgcgg ggagactggg cgttgctgac gtcgggatcg aaaagtttca
     2161 atattcccgc gctgaccggt gcttatggga ttatagaaaa tagcagcagc cgtgatgcct
     2221 atttatcggc actgaaaggc cgtgatgggc tttcttcccc ttcggtactg gcgttaactg
     2281 cccatatcgc cgcctatcag caaggcgcgc cgtggctgga tgccttacgc atctatctga
     2341 aagataacct gacgtatatc gcagataaaa tgaacgccgc atttcctgaa ctcaactggc
     2401 agatcccgca atccacttat ctggcatggc ttgatttacg tccgttgaat attgacgaca
     2461 acgcgttgca aaaagcactt atcgaacaag aaaaagtcgc gatcatgccg gggtatacct
     2521 acggtgaaga aggtcgtggt tttgtccgtc tcaatgccgg ctgcccacgt tcgaaactgg
     2581 aaaaaggtgt ggctggatta attaacgcca tccgcgctgt tcgttaaccc caattgcgca
     2641 acgtaaaaaa tcgttgcgca atcgtggatt tttaccctgc tttgttttta taatggtgcg
     2701 cacttttata tccagaaaaa gagtgcgacc atgattgata ccaccctgcc attaactgat
     2761 atccatcgcc accttgatgg caacattcgt ccccagacca ttcttgaact tggccgccag
     2821 tataatatct cgcttcctgc acaatccctg gaaacactga ttccccacgt tcaggtcatt
     2881 gccaacgaac ccgatctggt gagctttctg gccaaacttg actggggcgt taaagttctc
     2941 gcctctcttg atgcctgtcg ccgcgtggca tttgaaaaca ttgaagatgc agcccgtcac
     3001 ggcctgcact atgtcgagct gcgtttttca ccaggctaca tggcaatggc acatcagctg
     3061 cctgtagcgg gtgttgtcga agcggtgatc gatggcgtac gtgaaggttg ccgcaccttt
     3121 ggtgtgcagg cgaagcttat cggcattatg agccggacct tcggcgaagc cgcctgtcag
     3181 caagagctgg aggccttttt agcccaccgt gaccagatta ccgcacttga tttagccggt
     3241 gatgaacttg gtttcccggg aagtctgttc ctttctcact tcaaccgcgc gcgtgatgcg
     3301 ggctggcata ttaccgtcca tgcaggcgaa gctgccgggc cggaaagcat ctggcaggcg
     3361 attcgtgaac tgggtgcgga gcgtattgga catggcgtaa aagccattga agatcgggcg
     3421 ctgatggatt ttctcgccga gcaacaaatt ggtattgaat cctgtctgac ctccaatatt
     3481 cagaccagca ccgtagcaga gctggctgca catccgctga aaacgttcct tgagcatggc
     3541 attcgtgcca gcattaacac tgacgatccc ggcgtacagg gagtggatat cattcacgaa
     3601 tataccgttg ccgcgccagc tgctgggtta tcccgcgagc aaatccgcca ggcacagatt
     3661 aatggtctgg aaatggcttt cctcagcgct gaggaaaaac gcgcactgcg agaaaaagtc
     3721 gccgcgaagt aacaaaatgg atggtgcaaa ttgcaccatc catttttcat gcaaggcaca
     3781 aagtcgcgcg atgtttggcg gattcgatgc ccagctcaat caactccatt acctggattg
     3841 cctggcttgc cggaaccgga ttttcaccat cgccatttaa cgcatcacga atagccgcat
     3901 agtaagccgg ataattccca ggcaccgtca acagcgtttc ttcgacacgt tcctcacctt
     3961 ccacgcgggt aagtacgcca tcacgcatat cgtagcccca gtcttcctgc ggtagacgct
     4021 cgccattttt cagacgttct tcctgtggat cgaggccata tttcacataa ctgcctcggg
     4081 atccatgcac gatataccgt gctgactcag cggctgccag catggtaccg tgtaaaatga
     4141 ctcgccgctg tggataggac aagatggcgt ggaaataatc ggtcgactgc gctccgggcc
     4201 gtaactgtgc caaatctacc gtcatgctga ccggtaaacc aaaaagcgta atggcctgat
     4261 caagaagatg tggtgctaaa tcgtaccaga taccgctgcc tgggccgccc tgttcacgcc
     4321 aacgatcgcg cacctgcgga cggaagcggt caaaatgaga ctcaaagtaa gcaacttcac
     4381 ccagcacgcc ttccgcgagt aaacctttta gcgtcaagaa atcgctatcc cagcgacggt
     4441 tatggaatac agacagcaca cgccccaggc tttttgccag cgcatccagc tctcgcgctt
     4501 gtgacagtgt cacggtaaag ggtttatcaa cgaccacatg tttacccgcc tcaagcgccg
     4561 ctttggctaa cgggaaatgg gtatcgttgg gtgtaggaat gacaatcagg tctatgttgg
     4621 gatcgttaaa cagatgcttc ggctcagaga caaccgtaac cgttggccag tcggctttta
     4681 cttttgtttc atcgctgctg gagattaccg ccagttccag cccgggcgtg cccgcaatca
     4741 ggggcgcatg gaaggttttg ctcgcataac catacccaat caacccaaca cggatgttgt
     4801 cgctcatgtc attgcctctc attgaagtat gatggctatt tgacactatc ctttacccac
     4861 gctcaacagt ttaataacct gccagcaata agggatgttg tttaacttaa gtcaaaaaaa
     4921 tagcgaattt tccaacgaca aaagctaaat atcgcaaaaa cctcagtaaa aatcttgctg
     4981 gagctattat tgctaagtaa catttacccc ctgaagttaa tggatcaatc aagagagatg
     5041 tgggctgtaa tgaatcgtct tattgaatta acaggttgga tcgttcttgt cgtttcagtc
     5101 attcttcttg gcgtggcgag tcacattgac aactatcagc cacctgaaca gagtgcttcg
     5161 gtacaacaca agtaagctct gcacttgtgg agcgacatgc tgcccgtccg ggtgcatgtt
     5221 ttcacttgtc ggatattaaa ccaggaattt attatcttgt tcgatgttgt tggtgattgt
     5281 cagggatagt aaagcgcgtt actcttttca gagcagtttt tcatccctga atatttgcca
     5341 tcagcgcatt cttattattg taataacaca agcgttcact tttggtgaaa ttttatcatc
     5401 actaataata cctacccctt tggtattaat tattttatgg accttttatg actgttcagg
     5461 actacttatt aaaatttcgc aaaatcagtt cactcgaaag tctggaaaaa ctctacgacc
     5521 atcttaatta caccctgacg gacgatcagg aactgatcaa tatgtatcgt gctgccgatc
     5581 accgtcgcgc agagctggtt tctggcgggc gtttgtttga cctcggccag gtaccgaagt
     5641 ccgtctggca ctatgtccaa taaagaaagt agcgatcggt tataaaagcc ttataataac
     5701 gcccctaaaa ttagatttgc ctgtttgcgt cgctattctg gagaacctat gaccaccaca
     5761 acgccacaac gtattggagg ctggttgctt ggcccgttgg cctggctttt agtcgctctg
     5821 ttgagtacga cgctggcgtt gttgctgtat accgctgcgt tatcttctcc gcaaacattt
     5881 caaacgctcg gcggacaagc actgacgaca caaatcttat ggggcgtttc ttttattacc
     5941 gctatcgcca tgtggtatta cacactgtgg ttgaccatcg ctttctttaa acgtcggcgt
     6001 tgtgttccta aacactatat tatctggctg cttatttccg tattgctggc agtaaaagca
     6061 ttcgcttttt cacccgtgga agatggcata gccgttcgcc agttgctgtt tactttgttg
     6121 gcaaccgcat tgatcgtccc ctatttcaaa cgctcgtcgc gggtaaaagc gacgtttgtg
     6181 aatccgtaat aaccttacag ttaacctgtt gtcgcctgct ctggattaac ggataatagg
     6241 cggctttttt atttcaggcc gaaaaatgac tgactacctg ttactgtttg tcggaactgt
     6301 actggtcaat aactttgtac tggtcaagtt tctcggtctc tgtccgttta tgggggtttc
     6361 caaaaaactg gaaaccgcga tgggcatggg gctggcaaca acgtttgtga tgacgctggc
     6421 gtctatttgc gcctggctta tcgatacgtg gattttgatc ccacttaatc tgatttactt
     6481 gcgcaccctg gcatttattc tggtgattgc tgtggtcgtg cagttcaccg agatggtggt
     6541 gcgcaaaacc agcccggtgc tttaccgttt gctggggatt tttttgccgc ttatcaccac
     6601 caactgtgcc gtgctcggcg tggcgttgct gaatatcaat ctcgggcaca atttcttgca
     6661 gtcggcgctg tacggttttt ccgccgctgt cggtttttcg ctggtgatgg ttctcttcgc
     6721 agccatccgc gaacgccttg ctgtggctga tgtcccggca ccttttcgcg gtaatgccat
     6781 tgcgttaatt accgcaggtc ttatgtctct ggcctttatg ggctttagtg gtttggtgaa
     6841 gttgtaatga atgctatctg gattgccgtt gccgccgtga gcctgctggg cctggcgttt
     6901 ggcgccattc tgggttatgc ctcccgccgt tttgcggtgg aagacgatcc ggtcgttgag
     6961 aaaattgacg aaatcttacc gcagagccag tgtggtcagt gcggttatcc cggctgtcgc
     7021 ccctacgcgg aagccatcag ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc
     7081 gaagctgtga tgctaaaaat tgccgagttg cttaatgtcg agccgcagcc gctggatggc
     7141 gaagcgcaag agctaacgcc tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt
     7201 ggctgcacta aatgtattca ggcgtgtccg gtagacgcca tcgttggcgc tacccgtgcc
     7261 atgcatacgg taatgagtga tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg
     7321 acgcactgca tctcgttgca accggtcgca gaaacacctg actcctggaa atgggatctg
     7381 aacaccattc ccgtgcgtat cattcccgtg gaacaccatg cttaagttat tctctgcatt
     7441 cagaaaaaat aaaatctggg atttcaacgg cggcatccat ccaccggaga tgaaaaccca
     7501 gtccaacggt acaccactgc gccaggtacc actggcgcag cgttttgtta ttccactgaa
     7561 acagcatatt ggcgctgaag gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg
     7621 ccagccgctt acccgtggtc gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac
     7681 cgttacggct attgcgcccc actctacggc tcatccttca gctttagctg aattaagcgt
     7741 gattattgat gccgatggtg aagactgctg gatcccgcgc gacggctggg ccgattatcg
     7801 cactcgcagt cgcgaagagt taatcgagcg catacatcag tttggtgtag ccgggctggg
     7861 cggtgcagga ttcccgacag gcgttaaatt gcagggtggc ggagataaga ttgaaacgtt
     7921 gattatcaac gcggctgagt gcgagccgta cattaccgcc gatgaccgtt tgatgcagga
     7981 ttgcgcggct caggtcgtag agggtattcg cattcttgcg catattctgc agccacgcga
     8041 aattcttatc ggcattgaag ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct
     8101 ggcggactct aacgatattt ctctgcgggt gattccaacc aaatatcctt ctggcggtgc
     8161 taaacaatta acctacattc tgaccgggaa gcaggttcca catggcgggc gttcatccga
     8221 tatcggcgta ttaatgcaaa acgtcggcac tgcttatgca gtgaaacgtg ccgttattga
     8281 tggcgagccg attaccgagc gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg
     8341 caacgtctgg gcacggctgg ggacgccagt gcgtcattta ttgaatgatg ccggattctg
     8401 cccctctgcc gatcaaatgg tgattatggg tggcccgcta atgggcttta ccttgccatg
     8461 gctggatgtc ccggtcgtaa agattaccaa ctgtctgttg gctccctctg ccaatgaact
     8521 tggcgaacca caggaagaac aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc
     8581 tgcggatctt ttgccgcaac agttgtactg gttcagcaaa ggtcagcaac acgataaagc
     8641 taccacgcat aacattgctg attgcattga atgtggggct tgcgcgtggg tttgcccgag
     8701 caatattccc ctggtgcaat atttccgtca ggaaaaagct gaaattgcgg ctattcgtca
     8761 ggaagaaaag cgcgccgcag aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga
     8821 gcgcgaaaaa gcggctcgcc ttgaacgaca taagagcgca gccgttcaac ctgcagccaa
     8881 agataaagat gcgattgctg ccgctctggc gcgggtgaaa gagaaacagg cccaggctac
     8941 acagcctatt gtgattaaag cgggcgaacg cccggataac agtgcaatta ttgcagcacg
     9001 ggaagcccgt aaagcgcaag ccagagcgaa acaggcagaa ctgcagcaaa ctaacgacgc
     9061 agcaaccgtt gctgatccac gtaaaactgc cgttgaagca gctatcgccc gcgccaaagc
     9121 gcgcaagctg gaacagcaac aggctaatgc ggaaccagaa gaacaggtcg atccgcgcaa
     9181 agccgccgtc gaagccgcta ttgcccgtgc caaagcgc
//
LOCUS       DAJOWB010000157         9210 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJOWB010000157 DAJOWB010000000
VERSION     DAJOWB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.157"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OHO85_004224"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OHO85_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCQ2673507.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OHO85_004225"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OHO85_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCQ2673508.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /locus_tag="OHO85_004226"
     CDS             complement(2659..5772)
                     /locus_tag="OHO85_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCQ2673509.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLIKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="OHO85_004227"
     CDS             complement(5797..6954)
                     /gene="mdtE"
                     /locus_tag="OHO85_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCQ2673510.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7014..7292
                     /locus_tag="OHO85_004228"
     CDS             7014..7292
                     /locus_tag="OHO85_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673511.1"
                     /translation="MRDLQTSGIVGLSASKVGYRYSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="OHO85_004229"
     CDS             complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="OHO85_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCQ2673512.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="OHO85_004230"
     CDS             complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="OHO85_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCQ2673513.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT         2483 a         2123 c         2121 g         2483 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgcagtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagttttttc gctaagaaac
     1501 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcacag agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctctttta taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4441 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 tagccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5401 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atacattctg
     6061 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     6121 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     6181 gaatttcagc gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     6241 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgtaagaa
     6301 atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6361 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6421 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6481 cgctttggcg acggtgacat tggcttctgc ttcattcaac tgggtgcgcg cggtgtcgta
     6541 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6601 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6661 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6721 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6781 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6841 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
     6901 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6961 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
     7021 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggtatagtg
     7081 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     7141 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat aaagtcgttt
     7201 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     7261 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     7321 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     7381 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     7441 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     7501 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     7561 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     7621 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     7681 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     7741 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     7801 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     7861 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     7921 tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     7981 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagttt gtaaacagta
     8041 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     8101 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     8161 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     8221 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     8281 tgcaatgtaa tcaatgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     8341 tagcatcaac gcttacggaa ctctttgttc actttcccac tgcactttta aaacattaaa
     8401 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     8461 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     8521 atagtgcgca attgatctac aacgctgcgt agcggtgaga gtattaatcg gatcatagtc
     8581 acatcaagtg actatgatcc tggtgacaac cgggataatt attgctgctt aacgaacaaa
     8641 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     8701 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     8761 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     8821 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     8881 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     8941 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     9001 agcgcaatgc ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     9061 acgaacggga aactgataca cagcaacccg acgataaaca acagcacggc aataaactgg
     9121 attgctctgc gatgtttttt aagcatctcc agatcaaact tcaaaattgt tgccttatct
     9181 atatataaca tagaaccacc ctataaaatt
//
LOCUS       DAJOWB010000158         9150 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJOWB010000158 DAJOWB010000000
VERSION     DAJOWB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.158"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(473..646)
                     /locus_tag="OHO85_004231"
     CDS             complement(473..646)
                     /locus_tag="OHO85_004231"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673514.1"
                     /translation="MNGFKNDMDDEKSLKGKGATEHSNKTPEMQPERFLAMQINNNAK
                     TYNLITQLNHGCE"
     gene            826..1557
                     /locus_tag="OHO85_004232"
     CDS             826..1557
                     /locus_tag="OHO85_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCQ2673515.1"
                     /translation="MNQPIHNAYWLSRFDSLLDSALAQHRAVSLIRVDLRFPEYMPVT
                     IMDPDPDSAVISRFFASLKAKIQAYQRKKRCANQRVHATSLRYFWCREFGKMYGRKHY
                     HVILLLNKDTWCSLGDFSEPSSLATMIQEAWCSALHLEPWQGDGLVHFSRWTPSRKPA
                     SSDVRPSSDDTPLSGGCSDTWKASDKKSGEAAVLWIKRGDVEAMQKARNRASYLVKYE
                     TKQHDGSGQRNYGCSRGPGRLLDGR"
     gene            2067..2519
                     /locus_tag="OHO85_004233"
     CDS             2067..2519
                     /locus_tag="OHO85_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCQ2673516.1"
                     /translation="MNVIDSRIRDVKFGENVKVVMPVNLYECELGDDVFIGPFVEIQK
                     GCIIGNRSRIQSHSFICENVILGDGCFIGHGVTFANDLFKCGGPDASAESWIRIVLGN
                     TVTVGSGATILSTEICSGAVIGAGSVVTKPVLVKGVYAGNPARLLRTL"
     gene            complement(2962..4190)
                     /locus_tag="OHO85_004234"
     CDS             complement(join(2962..3875,3875..4190))
                     /locus_tag="OHO85_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCQ2673517.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSTRALIARGWGVSLVSRCLRGSRAQLHVILRRTDDWKDGRRSRHSDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRIYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMPGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRS
                     PESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQRACNGLSDNRCLEI"
     gene            4383..6431
                     /gene="ireA"
                     /locus_tag="OHO85_004235"
     CDS             4383..6431
                     /gene="ireA"
                     /locus_tag="OHO85_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HCQ2673518.1"
                     /translation="MKNKYIIAPGIAVMCSAVISSGYASSDKKEDTLVVTASGFTQQL
                     RNAPASVSVITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILV
                     DGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKP
                     VNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLIKDKLGLQLYGGMNYRKEDSIS
                     QGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQVHTLTPGESLDAWTMRGNLKQPNS
                     KRETHNSRSHWVAAWNAQGEILHPEIAVYQEKVIREIKSGKKDKYNHWDLNYESRKPE
                     ITNTIIDAKVTAFLPENVLTIGGQFQHAELRDDSATGKKTTETQSVSIKQKAVFIENE
                     YAATDSLALTGGLRLDNHEIYGSYWNPRLYAVYNLTDNLTLKGGIAKAFRAPSIREVS
                     PGFGTLTQGGASIMYGNRDLKPETSVTEEIGIIYSNDSGFSASATLFNTDFKNKLTSY
                     DIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVYDKWHVSANYTFTDSRRKSDDES
                     LNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVR
                     NGFTSMDIGLNYQILPDTLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRVSF
                     "
     gene            complement(6611..7775)
                     /locus_tag="OHO85_004236"
                     /pseudo
     CDS             complement(6611..7775)
                     /locus_tag="OHO85_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            7813..9026
                     /locus_tag="OHO85_004237"
                     /pseudo
     CDS             7813..9026
                     /locus_tag="OHO85_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    8094..8210
                     /locus_tag="OHO85_004237"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT         2332 a         2038 c         2291 g         2489 t
ORIGIN      
        1 taaaaccttt ctgtttaagc aatcaatctg ctattcgatt ttactgatgt tgcgtttaac
       61 cttttctgag atttcgagtt tcgacgttag catgagaaaa gtatctttac gctaaatcaa
      121 cccgaacttt cctgaagtaa tctgacgttc ttttcgatgt gcttacgacg attctgttaa
      181 acgactttct ggatgttttg ataacaatca gattgaggca ttaacgattc ctgacgtttt
      241 tcttttccgt gtaaggtcat tctttttgtc atgaggtttc ttcttgcatt gcgaaagtgt
      301 gatgaacggt tttggtgttt tgattcagat gagatttacg ttatcgatgg ttaatcgttt
      361 ttatggtgcg tagtgaaacg taacattttc gggatgagaa aatcgattct gatggtgatg
      421 gtgatggtga tgatcgtgtt acggcgttat ctgtaaacag gttttaaacg tgtcattcgc
      481 atccgtgatt gagttgtgtg attaagttgt aggttttggc gttgttgttg atttgcattg
      541 ccaggaagcg ttctggttgc atttctggtg ttttgttcga atgttctgtg gctccttttc
      601 ccttcaggct tttctcgtcg tccatgtcgt ttttaaatcc attcatgctg ttgttgcgta
      661 ttttgctgct gtgtgcagga ttatcttttc gttccgggat ggtttgttcc gtttcaatca
      721 ctcttaatca gagagttgta gtattgctca atgccggatg ccgtaaagtt ttactgcgtt
      781 gattaatgat gtgaagtcaa tccaaatcaa cggagatctc tcatcatgaa tcaaccaata
      841 cacaatgctt actggttatc ccgttttgac agccttctcg acagtgccct ggcgcagcac
      901 cgtgccgtct cgttaatccg ggtggattta cgtttccctg agtatatgcc tgtcaccatc
      961 atggatcccg atccggattc agcggtgatt tctcgttttt ttgcatccct gaaagccaaa
     1021 attcaggctt accagcggaa aaaaagatgt gccaatcagc gtgtgcatgc aaccagcctg
     1081 cgttatttct ggtgccggga gttcggcaag atgtatggca ggaaacatta tcacgtgata
     1141 ttactgctca acaaagatac ctggtgctcg ctcggggatt tcagcgaacc gtcttcgctg
     1201 gcgacgatga tccaggaggc atggtgcagc gccctgcatc ttgagccctg gcaaggcgat
     1261 ggcctggtcc atttttccag gtggacgcct tcccgtaaac cagcatcgtc tgatgttcgc
     1321 ccttcttccg atgacacgcc tttgtcgggt ggatgttctg atacctggaa ggcttcagac
     1381 aaaaagtctg gtgaagccgc tgttctctgg atcaagcgtg gtgatgtgga agcgatgcag
     1441 aaagcccgga acagagccag ttatctcgtg aaatatgaga cgaagcagca tgacggttcc
     1501 gggcaacgta attatggttg cagtcgtggg ccggggcgtt tgctggatgg caggtgaacc
     1561 gggtaaaacg gcatccggcg cctgagcgta taccacggta aggtcgttgg ctgacgccct
     1621 tagctcgttt tctgaaaaag tcgtcctgaa gtcatgtatc tgcttccatt ctcatgttca
     1681 tatttctctg acgctttcac tgtctgtcat cggaagtacc gatttatttt ggtgctgcac
     1741 acttacggcc attcacggaa gaggattgtg gtgaacagta ggagtctggc tgctggcgag
     1801 ggaaggttca ggactggtat ggggtttagt gctggaggat gactttgaat cccggattga
     1861 ccagtgaggg ggcagtgaga ggatgctctg gtgtaacagg cctggattgc aaaatataaa
     1921 accggttgta cactcgtaac gggcaaaagg atacgagcaa taacacatgg tacccggtga
     1981 gactggctct tgtatcacat taccgggaat attctgacag gtgaaatgtt ctgataagaa
     2041 tcatcctgag ggaggataca acggatatga acgttattga cagccgcatt cgtgatgtga
     2101 aatttggtga aaatgtgaaa gtcgtcatgc cagtaaacct ctatgaatgt gaactggggg
     2161 atgatgtttt tatcggtccc tttgtggaaa tacagaaggg atgtatcatt ggcaaccgga
     2221 gccgaattca gtcccacagt ttcatctgtg agaacgtcat tttaggtgat ggctgtttca
     2281 tcggtcatgg cgtaactttt gctaatgatc tttttaagtg tggtggtccc gatgcctctg
     2341 cagaaagctg gatacgcatc gtattaggta acactgtaac agtgggtagc ggagcgacca
     2401 ttttaagcac agaaatttgc agtggagcag tgattggtgc tggtagtgtg gttacaaagc
     2461 ccgtgctggt aaaaggcgtg tatgccggta atccagccag acttttgaga acactttaat
     2521 tatatttaac tcaataataa atcagacagt gaatataatt ctgccccgat tatactaatc
     2581 acgatgagta tcgatgagtc tcatatttat gctgttatta gattcattgc catagttatt
     2641 tattgcttgt cgctggttcg taagagcagg agcttaaaac tcggggagac gctgaatgcc
     2701 ataattggca gtacagaagg tgatatgtgt cgtcaacaaa aggagtcgtt cgctgccctg
     2761 acgcaacata aagcgtgcat tgatgctttt ctctgtggtt gtacagttat atgcgcaagg
     2821 acgggaacca gtctgccgat cctgcagcaa taacgggcaa tctccgtacg cccttattga
     2881 ttacacctgt gtgtaagttc acagagatat tgcaattgcc tccggataag taaggggaga
     2941 ttgcactatg tggatttgcc cctatatttc cagacatctg ttatcactta acccattaca
     3001 agcccgctgc cgcagatatt cccgtggcga gcgataaccc agtgcactat gcggatgcca
     3061 ttcgttataa tgctcgaacg cctctgcaag gttctttgct gccgttaacc cgtctggttt
     3121 gggcatgaca ctgatgtagt cacgctttat cgttttcacg aagctctctg ctatgccgtt
     3181 actctccgga ctccgcaccg ccgtgttctt cggttcaagc cccaacatcc gggcaaactg
     3241 ccgtgtttca ttagcccggt agcatgaacc attatccgtc agccactcta ctggagacgc
     3301 cggaagctcg ttgccgaagc ggcgttccac cgctcctggc atgacgtcct gtactgtttc
     3361 actgttgaag ccgcccgtag tgaccgccca gtgcagtgcc tcacggtcac agcagtccag
     3421 cgcgaacgtg acccgcagtt tttctccgtt atcacagcga aactcgaacc cgtcagagca
     3481 ccatcgctga ttactttctt tcacagccac tctgccggta tgtgcccgtt tcgatggcgg
     3541 tacagcgggt tttcgctcaa gcaacagcgc attctggcgc atgatccggt aaatacgttt
     3601 ggcattgatc gcaggcatac catcaagttc ggcctgtctg cgaagcagcg cccatacccg
     3661 acgataacca tatgtgggca gctctccgat aacatggtgt atacggagaa gcacatccgt
     3721 atcatctgag tgacggctgc ggcgaccatc tttccagtca tcggttcgtc tgagaatgac
     3781 gtgcaactgc gcacgcgacc cccggagaca acggctgact aagcttactc cccatccccg
     3841 ggcaataagg gcgcgtgtgc tatccacttt tttgcacgcc catattcaac ggcttcttta
     3901 aggagttcat tttccatcgt ttttttgccg agcaggcgct ggagttcttt aatctgcttc
     3961 atggcagcag caagttcaga ggcaggaacg acctgttctc ctgcggccac agcagtaaga
     4021 cttccctcct ggtattgctt gcgccagaga aataactggc tggctgccac accgtgttgc
     4081 cgggcaacaa gggagaccgt cattcccggt tcaaaactct gctgaacaat agcgatcttt
     4141 tcctgtgtag tacgccgtct gcgtttctcc ggtcctaaga catcaatcat ctgctctcca
     4201 atgactagtc taaaaactag tattaagact atcacttaaa taagtgatat tggttgtctg
     4261 gagattcagg gggccagtct actgaatgag cgaaatgtga tttaccttct tttctgttca
     4321 ttgtaaattc cctctttgct aacgcaaatc attatcatta cgcctttggc aaagggaaat
     4381 ttatgaagaa caaatatatc attgctccgg gcattgccgt gatgtgttct gcagttatat
     4441 catcaggtta tgccagttct gataaaaaag aagatacgct tgttgttact gcctccgggt
     4501 tcactcagca gctcagaaat gccccggcca gtgtctcagt cattacttca gaacaactgc
     4561 aaaaaaaacc ggtttcagat ctggtcgatg cagtaaaaga tgttgaaggg attagtatca
     4621 ctggtgggaa tgaaaaaccg gatatcagta tacgtggtct aagtggcgat tacacgctga
     4681 ttctggtcga tggacgacgt cagagcggtc gggaatccag accaaacggc agcggcggtt
     4741 ttgaagccgg atttatccct cctgtggaag caattgaacg cattgaagtg atccgtggcc
     4801 ctatgtcttc cctgtatggt tctgatgcca tcggaggggt cattaatatc ataaccaaac
     4861 cagttaataa ccaaacatgg gatggcgtac ttggacttgg ggggattatt caggaacatg
     4921 ggaaatttgg taactcaacc acaaatgact tctatctgtc aggcccattg attaaggata
     4981 aacttggtct tcagctatat ggaggaatga actatcgcaa ggaagatagt atctctcagg
     5041 gaacaccggc aaaagataat aagaatataa cggcaacgct ccagtttact ccgactgaaa
     5101 gccagaagtt tgtttttgaa tatggaaaaa ataaccaggt gcatacatta acacctggtg
     5161 agtctctcga tgcctggact atgcggggaa atcttaaaca accaaacagt aaaagagaaa
     5221 cgcataattc acgtagtcac tgggtagcag catggaatgc ccagggcgaa atactgcatc
     5281 ctgaaattgc tgtttatcag gagaaagtta ttcgtgagat taaatcaggt aaaaaagata
     5341 aatataatca ttgggatctt aattacgagt caagaaaacc ggaaataacc aacacaatca
     5401 tagatgcaaa agtgacggca tttctgccgg aaaatgtact gaccatcgga ggtcaatttc
     5461 agcatgcaga gctccgtgat gactcagcca cgggtaaaaa aacgacagaa acacagtctg
     5521 tttcaattaa acagaaagct gtttttatag aaaatgaata tgcagcaacg gattctctcg
     5581 ccctgactgg aggactgcgt ctcgataatc atgaaatcta tggcagttac tggaatccaa
     5641 gattgtacgc tgtttataac ctgaccgata atctcacact caaagggggg atcgcaaaag
     5701 catttcgggc tccttcaatt cgtgaggtga gtcctggatt tggaacactg acgcagggtg
     5761 gtgcctctat tatgtatgga aacagggacc tgaaaccgga gaccagtgta accgaagaga
     5821 tcggtattat ttatagtaat gatagtggtt tttcggcgag cgcgacgctg tttaatactg
     5881 attttaaaaa taagttgacc agttacgata taggtacaaa agatccagtc accgggttaa
     5941 acacttttat ttatgataat gtaggtgagg caaatatcag aggggtggag cttgcaactc
     6001 agattcctgt gtatgataaa tggcatgtat ctgcaaacta tacatttact gactctcgtc
     6061 gaaaaagtga tgacgaaagt ctcaatggca agtcgctgaa aggggaacct ctggaaagaa
     6121 ctcccagaca tgcagccaat gcaaaactgg aatgggatta cactcaggat attacatttt
     6181 attcatctct gaattatacg ggaaaacaaa tctgggcagc acaaagaaat ggtgctaagg
     6241 ttccccgcgt tcgtaatgga ttcacatcta tggatattgg tctaaattac cagattctgc
     6301 cagacacgct gattaatttt gccgttctta acgtcacaga cagaaagagc gaggatatcg
     6361 ataccattga tggtaactgg caggtcgatg aaggacgccg ttattgggct aatgtaagag
     6421 tatccttctg agagtatata aagacaacct gaggccgtga gcctcaggta ccagtgtagt
     6481 aatcagtaat gtcagtttcc aggttgtaat gcttttaatc ttgattaaaa taatcttaat
     6541 aatttaaact tgttaaatat cgatgatttt aatttgagag cgttcagaat tctgtgtcac
     6601 gttaaaaatt ctacagcgtc atcacattat tcagccgccc ttcaaaataa atggctaact
     6661 gcgacaatgt caaattccag ctttggatgg gcattgtcca tttctcctgt gcgttcatca
     6721 gtcccagata aagtagcttc aacaggctgt tttcattcgg gaaagcacct ttaggttttg
     6781 gtcagtttcc tgaactggcg gtggactgat tctatcgcgt tggttgtgta gatcaccttg
     6841 cggatattcg ccggatagcg gaagtacgct gacagatttt ccctcttgcg gcgccaggac
     6901 tggagcacca ccggatattg ctgcccccgt ttcgcttcca gctcatccag cgccgtttcc
     6961 gacaccgcac gatacactgg cttcaggtcg gccatgaacg ctttatggtg ttttgacgcc
     7021 acatatttaa tcgagttacg tatctggtga atgacgcaca actgtacctc tgtctgcgga
     7081 taaatgctgt ttatcgcttc agggaagccg gtcagcccat ccacgcaggc aatcagaatg
     7141 tcttcaacgc cacggttttg cagatcactt aatactgaca gccagaagtt agcgccttcg
     7201 ctttccgaca aatacaggcc caggacttct tttttgcctt ccagattcag cgccagtaag
     7261 gtataaactg ccttgctctg ataacaccca tcttcgcgga ttttatagtg aatggcgtcc
     7321 agccagacga agggataaac cttttccagc gggcgctgct gccactgttt taactcagga
     7381 atgactttat cggtaaccgc actgatggta gccgtggaaa cactgaaggc atataaatct
     7441 tcgatctccc ggctgatatc ctggtagctc atccccagcg cgaacaggcg aatgatcttg
     7501 cgctcgagct catcagacag cgtggtctgg tgcttcttca ccagttgggg ttcaaaataa
     7561 ccattacgat cacgtggagt tgccagctca aagctgccag tcggggcttt aatggtcttt
     7621 tttccggaac catttttacg attgacttca aaatcctgag ccagatggga gtcgagttca
     7681 gcggccagag cggtttctgt taactgtttg attaatggcg ttaggatacc atccttgccg
     7741 gttaatgctt ggccggactg aaccgccccg gaaatcctgg agactaaact ccctgagaaa
     7801 gaggtaaaca ggatgactaa aaatactcgt ttttcccccg aagtccgtca acgggcagtt
     7861 cgtatggttc tggaaagtca gggcgaatat gactcacaat gggcggcaat ttgttccatt
     7921 gctccaaaga ctggctgtac gccggagact ctgcgtgtct gggttcgcca gtatgagcgg
     7981 gataccgggg gcggagatgg agggctcacc agtgctgaac gtcagcgtct gaaagagctg
     8041 gaacgtgaaa atcgtgaact gcgccgcagc aacaatatcc ttcgccaggc ttccgcttat
     8101 tttgcgaagg cggagttcga ccgcctctgg aaaaagtgat ggcactgctg gataagctgc
     8161 gtgagcagta cggggtcaga ccggtatgca gtgaactgca tattgccccg tcaacgtatt
     8221 accactgtca gcaacagcga catcatcctg ataaacgcag tgcacatgca cagcgcgatg
     8281 actggctgaa gagagagata cagcgcgtat acgatgaaaa tcatcagtgt accgtgtgcg
     8341 taaagtctgg cgccagttgt tacgggaagg aatcaggatg gccagatgta cagtggtgcg
     8401 cctcatggcg gttatgggac ttgccggtgt tctccggggt aaaaaggtcc gcactaccgt
     8461 cagccggaaa accgttgccg caggtgaccg cgtaaaccgt cagttcgtgg cagaacgtcc
     8521 tgaccagctg tgggtggctg attttactta cgtcagcaca tggcagggct tcgtctatag
     8581 tggcgttcat cattgatgta tttgccggat atatcgtggg gtggtgggtc tcatcgtcta
     8641 tggaaacgac attcgtgctg gatgcgctgg agcaggcgtt gtgggcccgc cgtccgtccg
     8701 gcaccatcca tcacagcgat aaaggctctc agtatgtgtc actggcctat atggagcgat
     8761 taaaagaagc aaaactgctg gcatcaacag ggagtacagg cgactcgtat gacaacgcga
     8821 tggcggagag catcaatggt ctttacaaag cggaggtaat acaccgtaag agctggaaaa
     8881 accgtgcaga agtggaactg gccacactca cgtgggtgga ctggtataac aatcgacgat
     8941 tgctgggaag gctgggccat actcctccgg cagaagcaga aaaagcttat tatgcttcca
     9001 tcggaaacaa tgatctggca gcctgagttc acagataaaa cactctccag gaaacccggg
     9061 gcggttcagg actggagtgc cttaagcgct ttatcgaaat cgaagggttg ggacatgtgt
     9121 cattcctttt tggttgtata ttactggaat
//
LOCUS       DAJOWB010000159         9142 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJOWB010000159 DAJOWB010000000
VERSION     DAJOWB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.159"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..760)
                     /locus_tag="OHO85_004238"
     CDS             complement(<1..760)
                     /locus_tag="OHO85_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCQ2673519.1"
                     /translation="MLNMLIEPFRFDFINYALIIALLVSVPCALLSVFLVLKGWALMG
                     DAMSHAVFPGVVLAWIVGLPLGTGAFVAGLSCALLTGYIKDNSRIKQDTLLGIIFSGM
                     FASGLILYVSVRPDVPLEHILFGDMLGINATDILQTGTIALFILLITGIKWRDFLLYC
                     FDIQQARVSGLRTGWLYYGLLSMISLTVVATLKSVGIILSVSLLIAPGAIAVLLTQRF
                     SNALFIAVALSALVSTTGVYLSYFIDSAPAPTVVL"
     gene            complement(754..1614)
                     /gene="sitC"
                     /locus_tag="OHO85_004239"
     CDS             complement(754..1614)
                     /gene="sitC"
                     /locus_tag="OHO85_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004141220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCQ2673520.1"
                     /translation="MSWLLEPFGYQYMLNAMWISAMVGGICAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAFMLGLPFSVGAFVSGGLAAGSMFLLNQRTRLKEDVIIGLIFSSFF
                     ALGLFIISLNPVSVNIQSIILGNILAVPPEDILQLTVIGIISVITLLLKWRDLLLTFF
                     DENHARAVGLHPERLKILFFLLLTLSTVAALQTVGAFMVICLVVTPGATAWLLTDRFP
                     RLVCIAVTIGSVTSFLGAWASYYLDGVTGGIIVVAQTLIFLLVFIFAPVHGLLANRRS
                     VSQSKGPRKC"
     gene            complement(1611..2432)
                     /locus_tag="OHO85_004240"
     CDS             complement(1611..2432)
                     /locus_tag="OHO85_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese/iron ABC transporter ATP-binding
                     protein"
                     /protein_id="HCQ2673521.1"
                     /translation="MSQAAGITVAGLTVVYRNGHKALQNASFSVPKGSNAALLGVNGS
                     GKSTLFRAMMGFVSPSCGTISLLGMPPAQALRRNIIAYVPQSEEIDWSFPVLVEDVVM
                     MGRYGHMGMLRRAGKEDRRIVNDALERVDMLPYRHRQIGELSGGQKKRVFLARSIAQR
                     GKVILLDEPFTGVDVNTEKKIITLLGELKEEGSTLVVSTHNLTSVSEYCDYTVLVKGT
                     VLASGPVATTFTPENIKKTFSANIESESDILPVQGGSTQPNYQPSAEYEDKGRSS"
     gene            complement(2436..3350)
                     /locus_tag="OHO85_004241"
     CDS             complement(2436..3350)
                     /locus_tag="OHO85_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCQ2673522.1"
                     /translation="MLQLKTVKIILLSTFITGLTAIHGAYAAEKFKVITTFTVIADMA
                     QNVAGDAAEVSSITRPGAEIHEYQPTTGDIKRAQGAQLILSNGFNLELWFQKFYQRLK
                     NVPDVVVTTGITPISITEGPYDGKPNPHAWMSPDNALIYVDNIRDALVKYDQGNAETY
                     KANADAYKQKIRQTLEPLRKQIETLPAEQRWLVSSEGAFSYLARDLGLKELYLWPINA
                     DQQGTPQQVRNVIDKVRQNKIPAVFSESTVSNKPARQVARETGAHYGGVLYVDSLSAA
                     NGPVPTYLDLLNVTTRTVVQGLHSGMKE"
     gene            3609..5561
                     /gene="thrS"
                     /locus_tag="OHO85_004242"
     CDS             3609..5561
                     /gene="thrS"
                     /locus_tag="OHO85_004242"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCQ2673523.1"
                     /translation="MSEAPITESEPLTFTLPDGSLKHVRKGATLQNVAESIGSSVAKS
                     AVYAEIDGKCVDLLEKAKNSGKLNIITVFDEEALGPIRRGCLLLLAASVKQLFPSARM
                     VEGQLTKEGFYYDFAVDKPFTRDDLGMIEQHMTSLIAENPPIIKEQVTRAQAIDCFEQ
                     RGEKFKSTELMEILADKSVTLCHLRQFTDGFNGPLVPDLRFLKNMKLLNVSGAYWRGE
                     ANNVQLQRIYGTAWASKKQLDGWLEKTAEAEKRDHRKLGRELDLFHFQDNAPGAVFWH
                     PRGWTIFQSLIAYMRSRHEVAGYVEVNTPDVMDRSLWEISGHWDNYRDHMFTTQTEDG
                     RNFALKPMNCPGAVSLFKYGIKSYRDLPVRLSEFGKVHRYEPSGSLHGLLRVRHFTQD
                     DAHIFCTLQQVEGECKSILQLVLDIYKQFGFEEVAIKLSTRPEKRMGSDADWDRLENA
                     LSASLEAQGLQWSVNPGEGAFYGPKLEFVLRDAIGRDWQCGTLQVDMNLPERFDIGYI
                     AEDGSTKRPVMLHRALFGSLERFTGILLEHYVGKLPAWLSPVQAVVMTITDKQHHYAE
                     QVLKALRKKGLRCEADLRNEKIGFKIREQTLARIPFLLIVGDAEENAARVTVRDRTGR
                     CMGTLLLNEAADAIKICCQPPEVHLD"
     gene            5554..6405
                     /locus_tag="OHO85_004243"
     CDS             5554..6405
                     /locus_tag="OHO85_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallocarboxypeptidase"
                     /protein_id="HCQ2673524.1"
                     /translation="MIKQSRSDIPRRESGRHVWPVKEDDTQSNDIDFYKTAFREQSRR
                     WGLSEIILSNTGDGEISLYQSVNQGADVPHVLIAAGFHGEEPAGCWGMLDFLREGSPE
                     LFDNVAVSFLPLVNLSGLRTGSRLNSLGQNPNRGFTKGAEVEPSIEGKVLLNYETLLK
                     NAASHGVLCCHEDILRHKAYLYTFEHATRLGHFSVALRDELERFFPVMEKERVDGCEC
                     EDGIIFNHFDSSFESWLFSSCSDVAACTETPGLQPFAKRAEANRYLIGAFISSILERN
                     SIGSGMS"
     gene            6402..7748
                     /locus_tag="OHO85_004244"
     CDS             6402..7748
                     /locus_tag="OHO85_004244"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011154555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCQ2673525.1"
                     /translation="MKPLLLTNALIINEDLRYPADILIDKGRIQKIASLIPSRTEWQV
                     IDVRGKWVIPGMIDDQVHFREPGLTHKGTIASESAAAVMGGITSFMEMPNVTPPTTTL
                     QALREKFQRASHSSLANYSFYFGATNDNLDELKALTASQACGVKVFMGASTGNMLVDD
                     EQILESIFANAPCLVATHCEHTPTIKHNEETWRARLGDAIPAGEHAAIRSVDACLTSS
                     HQAVSLAKKHRTRLHVLHITTADELALFDAAPTLEALRQKTITAEACVHHLFFNYDDY
                     EMLGHKLKCNPSVKSSFHQEALWRGVNEGIIDVIATDHAPHLLEEKQNDYFAAPSGLP
                     LVQHALPALLDMSSRGIFTPEMVVRKTSHAVAERFQLKDRGYIREGYWADLVVIDPFS
                     HQQIIREDVAYKCGWSPFEGRILSGGAVDMTLVNGHVIWNGRTIQQKYGLPLEFCR"
     gene            7809..8831
                     /gene="hemB"
                     /locus_tag="OHO85_004245"
     CDS             7809..8831
                     /gene="hemB"
                     /locus_tag="OHO85_004245"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCQ2673526.1"
                     /translation="MSIIIPGSFPARRLRRSRTHSFSRRLVAENQLTVDDLILPVFII
                     EGQNLRQEVPSMPGVFRLSVDMLLHDAAQFVQAGIPAIALFPCIESSAKSLMADASWD
                     PSGLVPRAVRALKKAFPELGVITDVALDAYTTHGQDGITDENGYVLNEPTKEILTRQA
                     LTHAEAGADIVAPSDMMDGRIGHIRQAFETNGLLNIQIMAYSAKYASNYYGPFREATK
                     SAVSLGKRDKKSYQMDPANAMEAMHEIAQDLQEGADMVMVKPGMPYLDIIREARKTFA
                     VPVFAYQVSGEYTMHMAAFRNGWLDEEKTVLESLMCFKRAGADGILTYFANTVAQLLN
                     QHKREY"
     gene            8984..>9142
                     /locus_tag="OHO85_004246"
     CDS             8984..>9142
                     /locus_tag="OHO85_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673527.1"
                     /translation="MVETSKQDRMPYLLFLCENRILAQNIDGHVIDLGGLTKQNGTID
                     WLLDSNKEK"
BASE COUNT         2409 a         2138 c         2303 g         2292 t
ORIGIN      
        1 gcaaaacaac cgttggcgca ggcgcactat caatgaaata agagaggtaa acgccggtcg
       61 ttgataccag agccgataaa gccacagcga tgaataatgc gttgctaaat cgttgcgtca
      121 aaagaactgc aatagcaccg ggcgcgataa gcaaagagac ggataaaata atccccacgg
      181 actttagcgt ggcgacaaca gtaagcgaaa tcatgctcag taatccgtaa tagagccatc
      241 ccgtccgtag tccactgacc ctggcctgct gaatatcaaa acagtaaagc aggaaatcac
      301 gccatttaat tccggtaatc agcagtataa ataaggcaat ggtgcccgtc tggagtatgt
      361 cggtggcgtt tataccaagc atgtcgccaa agaggatatg ttcaagcgga acatccggac
      421 ggactgagac atacaaaatc agtccggagg cgaacatacc cgaaaaaatg atacccagca
      481 gcgtatcctg ctttatgcgg ctgttatctt taatatatcc ggtaagtaaa gcacaggata
      541 atccagccac aaaagctccg gtgcctaatg gcaaccctac aatccaggcc agtacaaccc
      601 cgggaaacac ggcatggctc atggcatccc ccatcagagc ccaacctttc agcacgagaa
      661 aaacagacag cagggcgcag ggaacagaga ccagcagcgc gattatcagg gcataattga
      721 tgaagtcaaa gcggaaaggt tcaatgagca tattcagcat ttacgcggtc cttttgactg
      781 agaaaccgat cggcggttcg ccagtaagcc atgtaccggg gcgaaaatga acaccagcaa
      841 gaaaatgagg gtctgagcga ccacgataat gccacccgtt acgccatcaa ggtagtaact
      901 tgcccacgca cccagaaagc tggtgacact gcctatggtc accgcaatac agaccaggcg
      961 ggggaagcga tcagtaagca gccaggccgt tgcgccaggc gttaccacaa ggcagatcac
     1021 catgaacgcg cctacggtct gtaatgccgc aacggtggag agggttaaca gaaggaaaaa
     1081 taatatctta agcctttccg gatgtaatcc taccgcccgc gcatgatttt catcgaaaaa
     1141 ggtcagtaat aaatctctcc attttaataa caacgtaata accgaaataa tgccaatgac
     1201 ggtaagctga agaatatctt ctggcggaac cgccaggata tttcccagaa tgattgattg
     1261 tatatttacc gaaacaggat tcagcgagat gataaataat ccaagcgcga aaaaggatga
     1321 aaagatcaaa ccaatgatga catcttcttt tagacgggta cgttgattaa gaaggaacat
     1381 gctgcctgct gccagcccgc cagaaacaaa cgcacccacc gagaagggca gtcccagcat
     1441 aaaagcgcca gctactccgg gtacgataga atgggacagc gcatcgccaa ttagtgacca
     1501 acccttcaac atcagataac aggataaaaa agcacatatt cctccgacca ttgccgaaat
     1561 ccacatggca ttgagcatgt attgataacc aaaaggctcc agaagccagc tcatgatgac
     1621 ctgcctttat cttcatattc agcggaaggt tgataatttg gctgtgttga tcctccctga
     1681 actggcaata tatctgactc actctcaatg tttgcactaa acgtcttttt tatattttcc
     1741 ggcgtaaatg tcgtggcgac aggaccgctt gccagcacgg tgcctttcac tagaacggta
     1801 taatcacaat attcggatac ggaggtaagg ttatgcgttg aaaccaccag ggtgctgcct
     1861 tcttctttca gttcgccaag cagcgtgatg attttctttt cggtattgac atccacaccg
     1921 gtaaaaggct catcaagaag gattactttg ccccgttgtg caatagaacg cgcgaggaac
     1981 acccgcttct tttgccctcc ggataattca ccaatctgcc ggtggcggta aggaagcata
     2041 tctacacgct caagcgcatc attaacgatc ctgcgatctt ctttacccgc tctacgcagc
     2101 atacccatat ggccataccg gcccatcatg acgacatctt ccaccaatac cgggaacgac
     2161 cagtcaattt cctcggactg aggtacatag gcaataatat tgcggcgaag cgcctgtgca
     2221 ggaggcatcc cgagaagtga aatcgttccg catgaagggg atacgaatcc catcattgcc
     2281 ctgaatagtg tggattttcc ggaaccattt acccccagca gggccgcgtt cgaaccttta
     2341 gggacgctga atgatgcatt ctgtaatgcc ttatggccgt tacgataaac aacagttaag
     2401 cccgcaactg tgatgcctgc cgcctggctc atcttttact ccttcattcc gctgtgcaaa
     2461 ccctgtacca ctgtccgggt agtgacgttc agcagatcaa ggtaggtggg aaccgggcca
     2521 tttgctgcgc tcagggaatc cacataaagt acgcccccat aatgtgcacc tgtttcgcgt
     2581 gcgacctgac gggctggttt attggaaacg gtactttcgc taaataccgc cggaatttta
     2641 ttctgccgga ctttatcgat tacgttacga acctgttgcg gggttccctg ttgatcggca
     2701 ttaatcggcc atagatagag ttctttcagt cccagatcgc gtgccagata agaaaatgcg
     2761 ccttcgctcg ataccagcca gcgctgttcg gcagggaggg tttctatttg tttacgcagc
     2821 ggttcaagcg tctgacggat cttttgttta taggcgtccg cattggcttt gtaagtttca
     2881 gcatttccct gatcgtattt caccagcgca tcacgaatat tatctacata aatcagtgca
     2941 ttatcaggcg acatccaggc atgaggattt ggttttccat catacggacc ctcggtaatg
     3001 cttattggag tgatacctgt agtgaccacc acatccggta cgtttttcaa tcgttgataa
     3061 aatttttgaa accagagttc gaggttgaag ccatttgaaa ggattagctg tgccccctga
     3121 gcacgcttta tatctccggt tgtaggctga tattcatgga tttctgcgcc aggccgggta
     3181 atcgaactga cttcggcggc gtcccctgcc acgttctgtg ccatgtcagc aattaccgtg
     3241 aatgtcgtga tgaccttaaa cttttcagca gcataagcgc catgaatagc tgtcagtcct
     3301 gtgataaagg tgcttaacag gattatcttt accgtcttta gttgaagcat ggttatctcc
     3361 tgatgaaatc actgtttgtt aataattggt aatgttataa catagcttat actatgcaac
     3421 atagcacagg ctatatttaa atgagaaaaa ttatctttat catttaagag ctgatggcac
     3481 agtatttcat ggcttatgca agcgctgata tctcagacat gccactttta atgcaacatg
     3541 gttgcgttaa aatgtgcatt aatgcaacat agttgctgtt aatcactgag ctatgttggg
     3601 atatgaaaat gtcagaagcg cctataacag agtcagaacc tctcactttt actcttcctg
     3661 atggctctct gaagcacgtc agaaaagggg caactttgca gaacgttgca gaaagcatcg
     3721 gttcttccgt cgcgaaaagc gctgtgtacg cggaaattga tggtaaatgt gtggatctgc
     3781 ttgagaaagc aaagaacagt ggaaagctca atatcattac agtttttgat gaagaagcgt
     3841 tgggcccgat tcgccgaggt tgtcttctcc ttctcgcagc gtctgtaaaa cagctgtttc
     3901 cctcggccag gatggttgaa gggcaactga ctaaggaagg gttttattac gatttcgcgg
     3961 tagataaacc ttttactcgc gatgacctgg ggatgattga gcagcacatg acaagtctga
     4021 tcgctgaaaa cccgccgatt attaaagagc aggtcacgcg ggcacaggct attgactgtt
     4081 ttgagcagcg gggcgagaaa ttcaaatcaa cggagttgat ggaaatactg gcagataagt
     4141 cggtgacact atgccatctc aggcagttca cggacggctt taatggccca ttggttccgg
     4201 atcttcgttt tctaaaaaat atgaagttgc ttaacgtatc cggtgcttac tggcgtggtg
     4261 aagccaacaa tgtacagcta cagcggattt atggcacggc atgggccagc aaaaaacagc
     4321 ttgatggctg gcttgagaaa acggcagaag cggagaaacg tgatcatcgc aagctgggtc
     4381 gggagctgga tttgtttcat tttcaggaca atgcgccagg cgcggttttc tggcatccca
     4441 gggggtggac aatctttcag tcgcttattg cctatatgcg tagccgccat gaggtggcgg
     4501 gttacgttga ggtcaatacg cctgatgtca tggaccgcag cctgtgggaa atttccggtc
     4561 actgggataa ttatcgtgat catatgttta cgacacaaac cgaggatggc cggaatttcg
     4621 ctctcaagcc gatgaactgt cctggggcgg tatcgctgtt taaatacggg atcaagagtt
     4681 accgcgattt acccgttcgc ctgtctgaat ttggtaaagt ccatcgttat gaaccctctg
     4741 gatcgctgca tggcttatta cgggtccgcc attttactca ggatgacgcg catatctttt
     4801 gcacgctgca acaggtcgag ggggaatgta aaagtatcct tcagttggtg cttgatattt
     4861 ataagcaatt tggatttgaa gaggtcgcaa tcaagttatc cactcgtcct gaaaagcgga
     4921 tgggaagcga cgcagactgg gataggctgg aaaacgcgct ctcagcctct ctggaagcgc
     4981 agggattaca atggtcggta aatcccggtg aaggtgcctt ctatggccct aaactggagt
     5041 ttgtgttacg cgacgcgatt ggtcgtgact ggcagtgcgg aacgctacag gtggatatga
     5101 atctgcctga gcgatttgat attggctata ttgccgagga tggctcgaca aaacgcccgg
     5161 tgatgttgca cagggcgctc ttcggctccc ttgagcggtt caccggtatc cttcttgaac
     5221 attacgtcgg taaactgccc gcctggctgt cgccggttca ggccgtggtc atgacgatta
     5281 ccgacaagca gcatcactat gcggagcagg ttttgaaggc gcttcgcaag aaagggctgc
     5341 gttgtgaggc cgacctccgc aatgaaaaaa ttggatttaa aatccgtgaa cagactctgg
     5401 cccgcattcc tttcctgctg atcgttggcg atgcggagga gaatgcggcg cgtgtcacgg
     5461 tgcgcgatcg taccgggagg tgcatgggga cgctgttact caatgaggcg gcagacgcta
     5521 tcaaaatatg ttgccagcca ccggaggttc accttgatta agcaaagcag aagcgatatc
     5581 ccccgacgcg agagcggtcg tcacgtctgg ccggtgaagg aggatgatac ccaaagcaac
     5641 gatatcgatt tttataaaac ggcgttcaga gaacagtcca ggcgatgggg gctttccgag
     5701 atcattctta gtaatactgg tgatggagaa atatcactct atcagtctgt taatcagggg
     5761 gcagatgttc ctcacgtact gattgcggca gggtttcatg gagaagaacc cgcgggatgt
     5821 tgggggatgc tggatttttt gcgcgagggc tcccctgaat tgtttgataa cgtagccgtg
     5881 agttttttac cgcttgtaaa cctctctggt ctgcgcactg gtagccgcct taatagtctt
     5941 ggacagaatc caaaccgagg ctttaccaaa ggtgcagaag tcgagccttc tattgaagga
     6001 aaagtactgc tcaactatga aacgcttctg aaaaacgcag caagtcatgg tgtgctctgt
     6061 tgccatgaag atattcttcg tcataaggcg tatctctaca ctttcgaaca tgctactcgc
     6121 ttgggtcatt tcagcgtggc attacgtgat gaacttgaac gctttttccc ggtcatggaa
     6181 aaagagcgag ttgatggttg tgaatgtgag gatggcatta tttttaacca tttcgattcg
     6241 tcatttgaat catggttgtt ttcttcctgt tccgacgtcg cagcgtgcac tgaaacgccg
     6301 ggattgcaac cctttgcaaa acgagcagaa gcaaaccgtt atttaatcgg cgcgtttatc
     6361 tcatcgatac tggagcgcaa cagcatcggg agcgggatgt catgaagcct ttattactga
     6421 ccaatgcatt aataatcaat gaagacttgc gctatccggc cgacatcctc attgataaag
     6481 gacggatcca gaaaattgcc tcgcttattc cctcccgaac ggaatggcag gttattgatg
     6541 tgcgagggaa atgggtgatc cccggtatga ttgacgatca ggttcatttc cgcgaacccg
     6601 gtctgacgca taagggaacc attgccagtg aatcagctgc cgctgtaatg ggaggcatta
     6661 ccagttttat ggaaatgcct aacgttacgc caccaacaac gacccttcag gcgctgcgtg
     6721 agaaatttca acgtgcctcg cattcttcgc tggcgaacta cagtttttac ttcggcgcga
     6781 cgaacgataa tcttgacgaa ctgaaagcgc ttacggcttc gcaagcctgt ggggtgaagg
     6841 tgtttatggg agcttctacc ggaaatatgc tggtggacga tgaacagatc ctggaaagta
     6901 ttttcgccaa cgcaccctgc ctggtagcaa cacattgcga gcataccccg acaataaagc
     6961 ataatgagga gacatggcgg gctcgtttgg gggacgctat tcctgctggc gaacatgccg
     7021 ctattcgttc tgtggacgcc tgcctgacat cttcccacca ggccgtctct ctggcgaaaa
     7081 aacatcgtac ccgattacat gtcctgcata tcaccaccgc agatgaactc gctctctttg
     7141 atgctgctcc cacgctggag gcgctgcgcc agaaaaccat tacagctgaa gcctgcgttc
     7201 atcacctgtt ttttaattat gacgattatg aaatgctggg gcataagctc aaatgtaatc
     7261 catccgtaaa atcctctttt catcaggaag ccctctggag aggcgttaat gaaggcatta
     7321 ttgacgttat cgcgactgac catgcgccgc atttgctgga ggaaaagcag aatgactatt
     7381 ttgctgcgcc ttccggctta ccgctggtgc agcatgctct tcctgcactg ctggatatga
     7441 gcagcagggg gatttttacc ccggaaatgg tggtgagaaa gacaagccat gctgtagccg
     7501 agcgcttcca gctcaaagat cgtggctata tcagagaagg ctattgggcg gacctggtgg
     7561 ttatcgatcc gtttagtcat caacagataa tccgggagga tgtggcatac aaatgcgggt
     7621 ggtccccctt tgaaggccgg attttgtcag gcggggcggt tgatatgacg ctggtaaatg
     7681 gccacgttat ctggaatggg cgcaccatac agcagaagta cggcctgcca ctggaattct
     7741 gtcgctagcc acgtatttat tttgctcagg cataacttct ccccgagaat tcagaagagg
     7801 caaatcttgt gagtattatt attcctggtt ctttcccggc acgacgactg cgccgttctc
     7861 gcacccattc tttcagtcgc cgtctggtcg ctgaaaatca gctgaccgta gatgacctga
     7921 ttttaccggt attcattatt gaggggcaaa acctgcgaca ggaagtgccg tcgatgccgg
     7981 gcgtatttcg tctgtcggtt gatatgttgc ttcatgatgc cgcgcaattt gttcaggcag
     8041 gtatacctgc tattgcacta tttccgtgta tagaaagttc tgctaaatcg ctgatggcag
     8101 acgcttcgtg ggatccatcc ggtttagtac cgcgagctgt aagggcgctg aaaaaggctt
     8161 ttcctgaact cggggtgata actgacgttg ctcttgacgc ctatacgaca catggtcagg
     8221 acggcatcac tgacgaaaat ggatatgtac ttaatgagcc gacaaaagaa attctcacca
     8281 gacaggcgct tacccacgcc gaagcaggcg cagatattgt cgctccaagc gacatgatgg
     8341 atggccgcat cggtcatatc agacaagcgt ttgaaacgaa tggactgctt aatatccaga
     8401 taatggcgta ttcagcaaaa tacgcgtcga actattatgg tccttttcgc gaggcgacga
     8461 aatctgctgt atcactgggt aaacgggaca aaaaaagcta tcagatggat ccggcgaatg
     8521 caatggaagc tatgcatgaa attgcccagg atttgcagga aggggcagac atggtgatgg
     8581 ttaagccggg catgccttac ctggatatta tcagggaagc caggaaaact tttgcagtac
     8641 ccgtctttgc ctatcaggtt tcaggggaat atacgatgca tatggccgct ttccgaaatg
     8701 gttggctgga cgaagaaaaa acggtgcttg aatcacttat gtgcttcaaa cgcgccgggg
     8761 cggatggaat actgacttat tttgccaata cagtagccca gttgcttaat caacataaac
     8821 gggaatattg attccctgtt gacaaaattc cttagtcgct aagcaggagc accgctgaga
     8881 gtaaacgtca taatcagcga gaatttctat actttaattc ttatctttat tgcgaattct
     8941 gacgaatcgt ttgtatcagc ccaataatct ggaggaagca gttatggtcg aaaccagtaa
     9001 acaggaccgt atgccttatc tacttttcct gtgtgaaaac cgcattcttg cacagaatat
     9061 cgatggtcat gtcattgatc ttggtggatt aaccaaacaa aacggcacca ttgactggct
     9121 tctggatagc aataaggaaa ag
//
LOCUS       DAJOWB010000160         9038 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJOWB010000160 DAJOWB010000000
VERSION     DAJOWB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.160"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(175..1548)
                     /gene="cobA"
                     /locus_tag="OHO85_004247"
     CDS             complement(175..1548)
                     /gene="cobA"
                     /locus_tag="OHO85_004247"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCQ2673528.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(1567..2373)
                     /gene="nirC"
                     /locus_tag="OHO85_004248"
     CDS             complement(1567..2373)
                     /gene="nirC"
                     /locus_tag="OHO85_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCQ2673529.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            complement(2501..2827)
                     /gene="nirD"
                     /locus_tag="OHO85_004249"
     CDS             complement(2501..2827)
                     /gene="nirD"
                     /locus_tag="OHO85_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCQ2673530.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            complement(2824..5367)
                     /gene="nirB"
                     /locus_tag="OHO85_004250"
     CDS             complement(2824..5367)
                     /gene="nirB"
                     /locus_tag="OHO85_004250"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCQ2673531.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANLQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            complement(5629..6810)
                     /gene="tsgA"
                     /locus_tag="OHO85_004251"
     CDS             complement(5629..6810)
                     /gene="tsgA"
                     /locus_tag="OHO85_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCQ2673532.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            7081..7653
                     /gene="ppiA"
                     /locus_tag="OHO85_004252"
                     /gene_synonym="rotA"
     CDS             7081..7653
                     /gene="ppiA"
                     /locus_tag="OHO85_004252"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCQ2673533.1"
                     /translation="MFKSTLAAMAAVFALSALSPTAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            7758..7925
                     /locus_tag="OHO85_004253"
     CDS             7758..7925
                     /locus_tag="OHO85_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCQ2673534.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRSHYER"
     gene            7915..8517
                     /locus_tag="OHO85_004254"
     CDS             7915..8517
                     /locus_tag="OHO85_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCQ2673535.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            8549..>9038
                     /gene="pabA"
                     /locus_tag="OHO85_004255"
     CDS             8549..>9038
                     /gene="pabA"
                     /locus_tag="OHO85_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCQ2673536.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLE"
BASE COUNT         2211 a         2371 c         2352 g         2104 t
ORIGIN      
        1 aacgatacag gaatatttaa ttggcgtcag tgagcgaatg acttaatttt gtaagtcgct
       61 gtaacggtga atataaccca ttagcagata atatttatta aggggctatg ttttatttgc
      121 tgaatatata aaaagagtca ttaagaaggc cagaattctc tggcccgggt taaattaatg
      181 gttggagaac cagttcagtt tatcgcgcag gccaacaacc cgaccaataa taattagcga
      241 tggactgttc atttgctgag caagttcacc gagttgcgtg agcgtaccgt caatcacgcg
      301 ctgcgtgact gccgttccgt tttcgacaat tgccaccggc atttcgccag gcataccgtg
      361 ttcaatcagc ttttgctgaa tagtcgcggc ctgattcaac cccatataga acaccagcgt
      421 ctgtttttct gccgccaggt tttcccagtc cagctcgcca ccggttttta agtgtccggt
      481 aattaagcgt acgctctggg cataatcgcg atgcgtgagt ggaatacccg aataggcaga
      541 gcaaccagaa gctgcggtaa tacccggaac caccgagaac ggaatacccg cgttgcacag
      601 tgtttccagc tcttcgccac cacggccaaa aataaacgga tcgccacctt tcagccgcac
      661 cacgcgtttg cctttttgcg cttcccgcag caggatctgg ttaatctctt cctggggtac
      721 gcagtggtat cccgcgcgtt tgccgacgaa aacacggtcc gcatcgcggc gtaccagatt
      781 cataatatcg tcagaaacca gacggtcgta gaccaccaca tctgcctgct gaatttgttg
      841 cagtcctttc agtgtcagca gcccggcatc gcccggacct gcaccaacca gcaccacttc
      901 accgcgatgg tcgagcggtt cgttgattaa ctgttcggtc gtttcagtaa tggctttctg
      961 atcgttgttt gccagcgact gcgccaggcg gtcgttaacg aacaatttct cccagaaacg
     1021 gcgacgctca cccatcgtgg cgaactgttg tttcactcgc ccgcgtaatt gcccggcgta
     1081 tttcgctacc tggcccagat gtaacggcag cagtgattca agtttttcgc gcaacaggcg
     1141 tgccagaacc ggagaggtgc cgccagagga gaccgctacc atgagcggtg agcggtcaat
     1201 aatcgacggc ataataaagc tggcggcttt cggcgcatcg accacgttac agaagatgcg
     1261 acgagcttca gcggcttcgc tgacgcgctg gttaagcgcg tcatcatccg tcgctgcaat
     1321 agccagccag caggtgtcga gaaggctttc atcaaatggc ccttcgacga gggttaacat
     1381 gcctgcatct gcccatgcgg tgaactgtgg aataaacgct aatgcattca ccgttaagcg
     1441 agcgcctgcg tctaacagca accttgcttt gcgttccgcg acatcaccac cgccgacaat
     1501 cagacagtcg cgatcgcgta attggcaaaa tataggcaaa tgatccacgt aaaaacccct
     1561 tagtaattaa ccggcagccg tttcagtttg attaaatttg tccgcaaccg gacgattcgc
     1621 tttcggcgta gcataccaat aacccaatcc catgaatacg gcacctgata aagtattacc
     1681 cagcgtcacc cacagcaggt tatgaccaat acccgccagc gtgtaggctt cgctgtggtt
     1741 gccgaaccag gagagcgcga acagcgtcat gttagcgata gagtgctcgt agccggacgc
     1801 gataaatgcc agcagacacc accagatagc gataaatttc gccgcccctt cagtgcgcag
     1861 cgccatccag attgccaggc aaaccagcca gttacacaat gcacctttga agaacagtac
     1921 cattgccggt gcagtggttt tagccagcgc gacggagtga acgatgctgg tatctaccgg
     1981 cagcaggcta ccgccgcccc agctatagag catggcaacg aagacggaac cgaccaggtt
     2041 acccagccag gtttgcggca ggattgccca catttgcccg tggctgatgc tgcccgcttt
     2101 taccccaaag gtgaggaaca tggtgtgtcc ggtgaacagt tcagaaccgg cgataatcac
     2161 cagcgttaag gcgataccaa aggtcgcgcc catcaccagc ggacgtacgg acgggtcgag
     2221 caaattaccg agcgtgaaaa tcaagatgat cccaagaccc acatacgcgc ccgccatggc
     2281 ggagctgacc caaaagccga gcgggttatt tgccgacagg cgtgcaatgc gcgcagcgtt
     2341 agccgcacac ttattaatag tgtctgtaaa catttgatta tcctttaaaa aataaaaaca
     2401 aaaaaaataa aacaaaaagt tttaaaaatg tcctgcaata taccgaaggt cattacagga
     2461 ccatgcaaaa aggggaggca ttgcgcctcc cgttaaaaca ttaaccgcgc agctgcacca
     2521 cgccgtcttt cactcgtgct tcgtaatgtt tgacggaaaa ctgttcgtct tccatgcaca
     2581 agccatcact taagcgaaaa cgctgttttt tcagcgggct ggcgacccac agctcgccct
     2641 ggtgttccgc aatcagcccg cgtgacagca cgctggactc gaagaacgga tcgatgttgc
     2701 tgatcgcaaa cacctggtcg ctgtgatacg ggcggaaaat cgcgacctgc tcgtcgccta
     2761 acagcgcgca gacgccggtt tcaggcagga tgtcatcgat tttgcagatg tctttccact
     2821 ggctcatgcg ttgtcctcca ccagagttac cgggatacgt tcatacggcg ttgccggacg
     2881 gtgctgttcg cgctctggca ccatctgcac gttcgggtca cgcttgtcgc tgttgatgaa
     2941 gtgtttgaag cgagtctgcg cggacggtgt attgaccgtt tcagtccact cacacactac
     3001 cgcttcacgc aggcgcgcca tctcttcttc cagatgagcg ttcagcccca gtttgtcgtc
     3061 aatgatcacc gctttcaggt aatcgatgcc gccttcgagg ttttctaacc acggcgcggt
     3121 acgcgtcagt ttgtcggcgg tacggatgta gaacatcatg aagcggtcga gatatttgat
     3181 cagcgtttcg cgatcgatat ccgctgccag cagatccgca tgacgcggtt tcatgccgcc
     3241 gttaccgcaa acatacaggt tccagccttt ttcagtggcg ataataccca cgtctttacc
     3301 ctgagcttct gaacattcac gggtacagcc ggagacaccg aacttcattt tgtgcggcgt
     3361 acggatgcct ttgtagcggt tttccagttc cacgccgagg ccgacgctat cgccaacacc
     3421 gtagcggcac caggtgctac ccacgcaggt tttcgccata cgcagtgctt tcgcataggc
     3481 atgaccggtt tcgaagccag cttcaatcag ctgacgccag atttccggca gatcgtcttt
     3541 ctgtgcgcca aacatcgcca ggcgctggga gccggtgatt ttggtgtaga gattaaattc
     3601 acgcgcgata cgacccactg ccatcagccc ttccggggtg atttcaccgc ccggagaacg
     3661 cgggatcacc gagtaggtgc cgtctttctg gaggttagcg aggaagttgt cgttagaatc
     3721 ctgcagcgga gtatgttccg gcttcagaat gtattcgttc cagcaggagg ccagcagcga
     3781 accgacggtt ggtttacaaa cttcacaacc gtagcctttg ccgtgtttcg ccagcagttc
     3841 ttcgaaggtt ttaatgcctt caacgcggat caaatggaac agttcctgac gcgaataagc
     3901 aaagtgttcg cacaggttgt tgttaacttc gatgccctgt ttcgccagtt ccgcgttcag
     3961 tacctgagtg accagcggga tacagccacc gcagccagta cccgctttgg tttcagcttt
     4021 cagcgccgca actgtgtggc agcctttgtt gatggcagca atcagatcac ctttggtgac
     4081 gtcgaagcag gagcagattt gcgcgctgtc cggcagttta tcaacaccga tagacggctt
     4141 accgctaccc gagtgcgccg gcagaatcag agaatccggg ttttccggca gctcgatagc
     4201 attcagcacc agttgcagca ggttgccgta gtcgctggta tcgcccacca gtaccgcacc
     4261 gagcagggtt ttgttgtctt cgctgacaat caggcgtttg tagatctctt tgctttcgtc
     4321 gaggtaaacg tagctacgtg cgccaggcgt gcgaccgtgc gcatcaccaa taccgcctac
     4381 gtctacgccc agcagtttca gcttggcgct aaggtcagca ccttcaaagg cgttttcgct
     4441 accgagaata tggtcaacgg cgacctgcgc cattttgtag cctggtgcta ccagaccaaa
     4501 tacacggttg ttccagcttg cgcattcacc gatggcgtag atatccggat cggaagtctg
     4561 gcaggaatca ttaatgacaa tacccccacg cggagcaacg tccagaccac actgggttgc
     4621 cagcttatcg cgcggacgga taccggtaga gaagacgata aagtcgactt ccagttcgct
     4681 gccgtcggca aaacgcatgg ttttacgcgc ttcgacacct tcctgcacaa tctcaagggt
     4741 gtttttgctg gtgtgaacgc gcacgcccat actttcgatt ttgcgacgca gctgctcgcc
     4801 acccatctga tcaagctgtt ccgccatcag cataggggca aattcgataa cgtgggtttc
     4861 aatacctaag tttttcagcg cgccagcggc ttccagacct aacaggccgc caccaacaac
     4921 ggcaccgcgt ttgctgcgac gggcgcagga ttcaatggcg ttgaggtctt caatggtgcg
     4981 atagacaaag cagtcctgag tatcagaacc tttgattggt gggatccacg ggtaggaacc
     5041 ggttgccatg atcagcttgt cataaaaaac ggtacgtccg gcgctggagt gaatcacctt
     5101 ctcctgacgg ttgatggtga tagcgcgttc gccgaccaga actttgatgc cgtgtttctc
     5161 gtagaagcct tcgcgcacca gcgacagctc ttcggcggtg tggtgagaga agtaagacga
     5221 gaggtgtacg cggtcataag cgatgcgcgg ttcttcacag aaaacggtaa tatcaaagtt
     5281 ggccgcatca gatttatcaa gaagatcttc gataaagcga tggccgacca taccgttacc
     5341 gataattgcg agtctgactt tgctcatttt tgcctcgatt tcttttctat taccgcctac
     5401 cttaacgatt cagcaacccc gcttattgat gtaaatcaaa ttcaccttta tatactcctt
     5461 aaggggtata ttgctgattt gtataaattt tcttaagtca cggaattgtc tgatatttca
     5521 tgtttgtgca aaaaatagat aaaatgaacg ggtttataga aatagatgca cgtagcgggg
     5581 gaagagagga aaaggccgga cgacgatagt gccgtccggc attagcaatt aatgagaggt
     5641 cagggtgtta tgctgacggt gacggctgac gaaacctaac aggaagcaca tcacaaagac
     5701 gacagcgtac agaccgtttg ctgtaagcag tgccgcctgc ggaccgctat gttcaacaat
     5761 cgggccggta accacaaagg tcaacatagt accgatggtt ccgcaggtca ggacaaagtt
     5821 aaccagtttt ggcgacggta ctttggtctg ctgtgaaccc agggtgatga tggtggtata
     5881 gatcgcgctg gagaagaagc ccagagcgag aattgaccac gccatatgtg ctggtgttcc
     5941 ggtgttaaag acgtacatca gaatcgcagc cagaccagcc agtacggtca gaatgcgttg
     6001 caaatcaaag aagcgaagaa taaagctgaa cgcccacatg ccgaccatgt atgacatcca
     6061 gaagttactc accagcgtgc ccgcgtcgtt caggctcatg cccaggcctt tggcatactc
     6121 aggcacccag gagataaaac ctaactgacc gaggatgtag cacagcgccg caacagagag
     6181 aaacagtacg ccgatccccc acttttcttt ctctaccgga gcatccgttt ttggcgcatg
     6241 tttgcccagc gccgggaact cacagccgaa ggtcagaata aaaatagcga catacaccag
     6301 cccgatgcag gcataaaccc agtaccactc aatgctgcgc gccagtagaa acgcggcgat
     6361 cattgggaaa atcatcccag ccatactgaa gaaggagtcg gtaaataaca ggcgggaacc
     6421 gcgctgacgc ccttcataca tttgtgttac caggaatgta ccaatcgaca tggtgatgcc
     6481 gctgaccacc ccgagaatga acatcgccgc cgagaacagc gccaggctgt ggctgaacat
     6541 caaaccggca accgccagca ccatcaggag aaagccaaaa cgtaactgcg ttttcaacgg
     6601 gacgatttcc atcagccagg cgttgaggaa gatagagatt aaaatgccgg cgttgaggaa
     6661 ggtgaaggtg ttactcatac tggaaacagg cagattgaaa taatcggcga tatttcccat
     6721 caccatcccg gtgacaataa ccaacgcacc agtcagtgcg taggagagaa agctaatcca
     6781 tgtgagcttg atgcgattgc tgttagtcat gattggcctg cgttcaaaaa taaaatggca
     6841 tagcgggata tgccgcgagc gggcgatttt aggtaatttt gtgatctgtt taaatgtttt
     6901 attgcaatcg gttgctaaat tgcattttaa gaggtgattt tgatcacgga ataaaaagtg
     6961 atcgtcaggt tacatatatt tcagatacgt aaaattaggt aaagggatgg ccttgttctt
     7021 gaaggctatt tagaatctct tcacttgctt tttttctgct ctgtttgtta aggaaatctc
     7081 atgttcaaat cgaccctggc ggcgatggct gctgttttcg ctctttctgc tctttctccc
     7141 acagcaatgg cagcgaaagg ggacccgcac gtattgttga caacctcagc tggtaacatc
     7201 gaactggagc tggataaaca aaaagcgcca gtgtctgtgc aaaactttgt cgattatgtg
     7261 aacagcggtt tttataacaa cactaccttt caccgcgtca ttcctggctt tatgattcag
     7321 ggcggcggtt tcaccgagca gatgcagcag aaaaaaccaa acccgccaat caaaaatgaa
     7381 gccgataacg gcctgcgcaa cacgcgtggt accatcgcga tggcgcgtac cgctgacaaa
     7441 gacagcgcca ccagccagtt ctttatcaac gttgccgata acgccttcct tgaccatggt
     7501 cagcgtgatt tcggttacgc ggtatttggt aaagtggtga aaggcatgga cgtggccgat
     7561 aagatttccc aggtgccgac tcatgacgtt ggtccgtacc agaatgtgcc gtcaaaaccg
     7621 gtagttatcc tttccgctaa agtcctgccg taatgatttc tcgcgcgggc aatcttgccc
     7681 gcgcttctgc tctcccggcg taacccggat ttgccgctta tacttgtggc aaatggacac
     7741 gttcagggag gcatcaagtg aagaaactca ccgataagca aaagtcccgt ctctgggagc
     7801 ttcagcgtaa tcgtaatttt caggctagtc gccgtcttga aggcgtcgag atgcctttag
     7861 tcactcttac tgccgcagag gctttagcgc gccttgaaga gctgaggagt cactatgagc
     7921 gataaattcg gcgaagggcg cgatccgtat ctttatcctg gccttgatat catgcgtaac
     7981 cggctgaaca tccgccagca acaacggctg gaacaggccg cttacgaaat gacggcgctg
     8041 cgtgctgcga ccattgagct tggtccgctg gtgcgcggtt taccgcattt gcgcactatc
     8101 catcgccagc tgtatcagga tattttcgac tgggcagggc aactgcgtga agttgatatt
     8161 tatcagggcg atacgccgtt ctgccacttt gcttatatcg aaaaagaggg caatgccctg
     8221 atgcaggatc tggaggaaga aggttatctg gttggcctgg agaaagcgaa gttcgtcgag
     8281 cggctggcgc attactattg tgaaatcaac gtgctgcatc ccttccgggt gggaagtggt
     8341 ctggcacagc ggatcttctt cgagcaactg gcgattcatg ccgggtatca actgagctgg
     8401 cagggtatcg aaaaagaggc ctggaatcag gcaaatcaga gtggggcaat gggggatctc
     8461 accgcgctgc agatgatatt tagcaaagtg gtaagcgaag ccggggaatc tgagtaaaat
     8521 agcgcggttc tttcgtaccg gagccgccat gatcctgctt atagataact acgattcttt
     8581 tacctggaac ctctaccagt acttttgtga actgggggcg gatgtgctgg ttaagcgcaa
     8641 cgatgcgttg acgctggcgg atatcgacgc ccttaaacca caaaaaattg tcatatcacc
     8701 cggcccctgt acgccagatg aagccgggat ctcccttgac gttattcgcc actatgccgg
     8761 gcgcttgccg attcttggcg tctgcctcgg tcatcaggca atggcgcagg catttggcgg
     8821 caaagttgtg cgcgccgcaa aggtcatgca cggcaaaacc tcgccgatta cacataacgg
     8881 tgagggcgta tttcgggggc tggcaaatcc acttaccgtg acacgctacc attcgctggt
     8941 ggtggaacct ggctcattac cagcatgctt tgacgtgacg gcctggagcg aaacccgcga
     9001 gattatgggg attcgccatc gccagtggga tctggaag
//
LOCUS       DAJOWB010000161         9038 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJOWB010000161 DAJOWB010000000
VERSION     DAJOWB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.161"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            36..641
                     /locus_tag="OHO85_004256"
     CDS             36..641
                     /locus_tag="OHO85_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HCQ2673537.1"
                     /translation="MSNKELITEFLLAAEQGNSDGLKSCLDKKVDINATNREGRTAII
                     IASLNKHYDCVSLLISAGADINKQDQTCFNPFLIGCLNNDLTLLRTVLPANPDLNCLT
                     RFGGVGITPACEKGHVEIVRELLMRTDINVNHTNFVGWTPLLEAIVLNDGGEKQQEIV
                     KLLLEHGANPHMTDKYGKTPLELAREKGYHKIAELLISAGA"
     gene            727..1575
                     /locus_tag="OHO85_004257"
     CDS             727..1575
                     /locus_tag="OHO85_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCQ2673538.1"
                     /translation="MGQVEQVFSHAVNLLIPEQQRLLTLLSEDCDNAPNSCRLALTHY
                     EDLFHPGEQVNFSGSGITVGNDKWIEITGCQMWRIPRWTSDSASLAKVGWQHWEEFIR
                     QQLRSNDTLFLYQGDNPFYQEISRQLLNRRKNLLKALCTGENITAAVNQLIGLGIGLT
                     PSSDDYLVGLSTILFIQGHPLRKYRYDFLAALQLAGNNITLLSKITLEEAFNQRYRER
                     VGRLLNQFITEPQPISTQYVTDIKNIGSSSGCDMLYGMADACALSHRSGGNYVNQDSC
                     KKKHVF"
     gene            1535..3082
                     /gene="fdrA"
                     /locus_tag="OHO85_004258"
     CDS             1535..3082
                     /gene="fdrA"
                     /locus_tag="OHO85_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCQ2673539.1"
                     /translation="MSIRIVVKKNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEEAKSGDLMIVIKGTSEEANERTLIAIDELFAHKEQGGQHEARYA
                     TIASAKKHAPESNLAVISVNGLFAAREARQALQNNLNVMLFSDNVSLEDELELKHLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIIGASGTGSQELSVRIHEFGGGISQ
                     LIGTGGRDMSEKIGGLMMLDAISMLEADPQTEIITLISKPPAPAVARKVLERARTCHK
                     PVVVCFLGRESTPADEEGLQFARGTKEAALKAVLLTGVKKESLDLHPLNWQLIEEVRS
                     RLTPQQKYIRGLFCGGTLCDEAMFTAMEKLSDVYSNIHPNPAFRLTDLNRSVAHTFLD
                     FGDDYFTNGKPHPMIDPTNRITRLLQEAQDPEVGVIVMDFILGFGSHEDPVGVMLDTI
                     VEAKEIAAADGRPLEILGYVLGTDLDTPSLKKQCQMLTDAGVIWASSSTNTGLLAREF
                     IFKGEEG"
     gene            3082..4500
                     /locus_tag="OHO85_004259"
     CDS             3082..4500
                     /locus_tag="OHO85_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCQ2673540.1"
                     /translation="MSQTLFNQPLNVINVGIEMFSDDMKKQNVPVTQLDWTPPGQGNI
                     QVVTALDDIAASPLADKIAAANQLALERIIQSHPVLIGYDQAINVVPGMTRNTILHAG
                     PPIHWEKMCGAMKGAVTGALVFEGLAKDLDDAAELAASGEIIFSPCHEHDCVGSMAGV
                     TSASMFMHIVENKTYGNRAYTNMSEQMAKILRMGANDQSVIERLNWMRDVLGPMLRDA
                     MKLAGEIDLHLMLAQALHMGDECHNRNNAGTALLIQTLTPWIIQTGYPVEQQREVFEF
                     VLSSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRISGLPGQWFTGPA
                     QQVIGPMFAGYKLEDSGRDIGDSAITETYGVGGFAMATAPAIVSLVGGTVDEAIDFSR
                     KMREITLGENPNVTIPLLGFMGVPTAIDITRVGSTGILPVINTAIAHKDAGIGMIGAG
                     IVHPPFTCFEKAILNWRDHYCQ"
     gene            4613..6163
                     /locus_tag="OHO85_004260"
     CDS             4613..6163
                     /locus_tag="OHO85_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine permease"
                     /protein_id="HCQ2673541.1"
                     /translation="MNSIKLEWKRGDWAAYFGLMTNNLTNLLTMMGLLIFVVGIPTEI
                     VYGRIAPAFGLAVLAASVCYSWFGLQMVKKTGRSDVTALPSGPSAPSIFTVTFLVLMP
                     VYQQTKDANFAIQIGLVWCFVEALILVGGSFLGETIRKMIPRTVLLSCLSGLGLLLLA
                     MNPMLQAFEAPTVSFIVLLLIFINWFGKKPIFPHIPTGLLLLIAGTSLAWISGLQSPE
                     DIKASMSSFGFNPPEIHVRSFLQGLPHALPYLASAVPLGLANYIFDLENIESAHAAGD
                     EYNTRKVMMANGFASMLGCMLGNPFPVTVYVGHAGWKAMGASICYTLASGITMFLVPL
                     FGLGAFMLAIIPMTAIVPILVFIGVVTANQVVRETPKVEVPVIFICLFPWVANWALTI
                     VNSVMGAAGTSAEKLGSDLLHSKGVYYDGLMHLGSGAPLASMLWGCIAIFAIMNKPLR
                     GAIAASFGALLSLFGVIHSPAVGFAEGSSIVFVVAYLMMLAMFALKHVIDSRETVAAS
                     EQEETKTT"
     gene            6190..7152
                     /locus_tag="OHO85_004261"
     CDS             6190..7152
                     /locus_tag="OHO85_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HCQ2673542.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     PHGEKKAVTVVTQVEVDKNDPGFVHPTKPIGAFFSENQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKTLIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     SSALLAREIHADILVITTGVEKVSIHFGKPNQHALDTVDVLTMARYMQEGHFPPGSML
                     PKILASLEFLERGGKRVIITTPECLSAALRGETGTHIIHSQEET"
     gene            complement(7988..9038)
                     /locus_tag="OHO85_004262"
                     /pseudo
     CDS             complement(7988..9038)
                     /locus_tag="OHO85_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         2237 a         2173 c         2360 g         2268 t
ORIGIN      
        1 atttgtaaaa tcttaaaaag agcaggagat cgattatgtc caataaagaa ctcattacag
       61 aatttctgct ggcggcagaa caaggtaatt ccgatggatt aaaatcatgc cttgataaaa
      121 aggttgatat taatgcgact aaccgcgagg ggcgtactgc aattattatt gccagtctga
      181 ataaacatta tgactgcgtt tctttattga tttccgcagg tgctgatatt aataaacaag
      241 atcaaacttg ttttaatccc ttcctgattg gctgcctgaa taatgattta acactgttgc
      301 gtacggtatt accggctaac cccgacctta attgcctaac ccgttttggc ggtgttggta
      361 ttacacctgc ctgcgaaaaa ggccatgtcg agattgtacg tgaattgctg atgcgtactg
      421 atatcaatgt caaccatact aattttgttg gctggacgcc gctcctagag gccatcgtgc
      481 tgaatgacgg cggtgaaaag cagcaggaga tcgtcaaact gttgcttgag catggcgcaa
      541 acccgcacat gacggataaa tacggtaaaa ccccgctgga gctggcgcga gaaaaaggct
      601 accacaaaat tgccgagttg ctgatttcgg ctggagcctg acaacaggag cacgattatc
      661 gctatattat cacggcaatc ttttcgggca ctaaccgcag actgcgcgtt tctcactttt
      721 cggggaatgg gccaggttga acaggttttt tcccatgcgg taaatctgtt aatacctgaa
      781 cagcagcgac tgctgacgtt actgagtgag gattgtgata acgcacctaa cagttgtcgt
      841 ttagcactga cacattatga agaccttttt caccccggtg aacaggtcaa ttttagcggg
      901 tctggaataa ctgttggaaa tgataaatgg atagaaataa ccggttgtca gatgtggcga
      961 ataccacggt ggacatctga ttcagcaagc cttgccaaag tcggctggca gcattgggaa
     1021 gagtttatcc gccaacaact aagaagcaac gacacattat ttctgtacca gggtgacaat
     1081 ccattttatc aggaaatttc tcgccaatta ctcaaccgcc gaaaaaattt gctgaaggcg
     1141 ttgtgtacag gagaaaacat cactgcggca gtcaaccagt taattggtct tggaataggg
     1201 ctgacgccat catcggatga ttatttagta ggtttaagca ctattttatt tattcaagga
     1261 catccactaa ggaagtatag atacgatttc ttagcagcat tacaacttgc aggaaataac
     1321 ataacgcttc tcagcaaaat aacgttagag gaagcattta atcagcgtta tcgggaaaga
     1381 gtcggacggc tactgaacca atttattact gagccacaac ccatttctac gcaatatgtt
     1441 actgacataa aaaatatagg ctcaagttct ggatgcgaca tgctgtatgg catggcggat
     1501 gcctgcgccc tgagccacag gtccggaggt aattatgtca atcaggatag ttgtaaaaaa
     1561 aaacacgtat tttgattctg tttcactgat gtctatctcc acgcgcgcaa ataagcttga
     1621 tggagttgag caggcatttg ttgctatggc gacagagatg aataaggggg tgctgaagaa
     1681 tctcggtctg ctgacccctg agctggaaga ggcgaaaagt ggcgatctga tgattgtcat
     1741 taaaggtacc agcgaggagg cgaacgagcg gacgctaata gcgattgatg agctgtttgc
     1801 gcataaagaa cagggaggcc agcacgaagc acgctatgcc acgattgcca gtgcgaagaa
     1861 gcatgctcca gagagtaatc tggcggtgat ctctgtcaat ggcctgtttg ctgctcgtga
     1921 agctcgtcag gcactacaaa ataacctcaa tgttatgctg ttttctgaca acgtctctct
     1981 tgaggacgag ctggaactca agcatctggc acatgaaaaa gggttgttga tgatggggcc
     2041 tgattgtgga accgccatca ttaacggcgc agcgttgtgt tttggcaatg cagtgcgtcg
     2101 cggcaacatc ggaatcattg gtgcctccgg tacgggtagc caggagctga gcgtacgcat
     2161 tcacgaattt ggcggcggga tttcccagct gattgggaca ggtggacgcg atatgagtga
     2221 aaaaattggc ggcctgatga tgcttgacgc gattagcatg ctggaggccg atccacaaac
     2281 tgagatcatt acgcttatat ccaaacctcc tgcaccagcg gtagcccgta aggtgctgga
     2341 acgtgcgcgc acctgtcaca agcctgtggt ggtgtgtttc ctcggacgtg agtccacccc
     2401 tgcggatgaa gaaggcctac agtttgcccg cggtactaaa gaggctgcct tgaaagcggt
     2461 actgttgact ggtgtgaaaa aagagagtct ggatttgcat ccgctcaact ggcagctgat
     2521 tgaagaggtt cgctcccgtc tgactccaca acaaaagtat atccgtggcc tgttctgtgg
     2581 tggcacgtta tgcgatgaag cgatgtttac tgccatggaa aaactctcgg atgtgtatag
     2641 caatattcac ccgaatccgg cgttccgcct tacagatctc aaccgtagcg tggcgcatac
     2701 cttcctcgat ttcggcgatg attattttac caacggcaaa ccacatccga tgatcgatcc
     2761 caccaatcgc attacccggc tcttgcagga agcacaggat cctgaagttg gcgtgatcgt
     2821 gatggacttc attcttggat ttggatcaca tgaagatccg gttggagtga tgctggatac
     2881 aattgttgaa gccaaagaaa ttgctgccgc agacggtcgc ccgcttgaga tcctcggcta
     2941 tgtgctggga accgaccttg atacaccgtc gctgaaaaaa cagtgtcaga tgttgaccga
     3001 tgccggggtt atttgggcaa gcagtagcac caacaccgga ttactggcac gtgaatttat
     3061 atttaaaggg gaggaaggct gatgagccag acactgttta atcagccgct gaacgttatt
     3121 aatgtcggta tcgaaatgtt cagcgatgac atgaaaaagc agaatgtacc ggtaacccaa
     3181 cttgactgga caccgccagg ccaaggaaac atacaagtgg taacggcact ggacgacatt
     3241 gccgcttcac cactggcaga caaaattgca gctgctaatc agctggcgct ggaacgcatt
     3301 atccagtcgc atcctgtgct aattggctat gatcaggcga tcaacgtcgt gccgggtatg
     3361 acccgtaaca ctatccttca cgccgggccg ccaatccact gggaaaaaat gtgcggcgca
     3421 atgaagggcg cagtaaccgg ggctctggta tttgaaggac tggcgaaaga tctggatgat
     3481 gccgccgaac tagctgcttc aggtgaaatc atcttctctc cgtgccacga acatgactgt
     3541 gtcggctcaa tggcgggtgt cacgtctgca tcgatgttta tgcacatcgt cgagaacaaa
     3601 acctatggaa accgcgcata taccaatatg agcgagcaga tggcaaagat cctgcgtatg
     3661 ggcgctaacg accagagcgt tatagagcgt cttaactgga tgcgagatgt actgggacct
     3721 atgttgcgcg atgctatgaa gctggcgggc gaaatcgatc tgcacttgat gttagcgcag
     3781 gcgctgcata tgggcgatga gtgccataac cgcaacaacg ccgggacggc gctgctgatt
     3841 cagacgctga cgccgtggat tattcagact ggctaccctg ttgagcagca gcgtgaggtg
     3901 ttcgagtttg tcctcagtag tgattacttc tcaggaccaa catggatggc aatgtgtaaa
     3961 gccgcaatgg acgctgcgca cggcattgaa tacagcaccg tggtgacaac gatggcacgc
     4021 aatggtgttg agttcggtct tcgtattagc ggtttgccgg gccagtggtt taccggtccg
     4081 gcgcagcagg tgattgggcc gatgtttgcg ggctataagc tagaggattc cggacgtgat
     4141 atcggtgata gtgcaattac cgaaacctac ggcgttggcg gtttcgctat ggcgacggca
     4201 ccagctattg tgtcactggt gggcggaaca gttgatgaag ctattgattt ctcacgtaaa
     4261 atgagagaaa tcactctcgg agaaaacccc aacgtcacca ttcccctgct tggttttatg
     4321 ggcgtaccga ccgctatcga tattacgcgt gtaggcagta caggcattct gccggtaatt
     4381 aacacagcca ttgcgcacaa agacgcaggg atcggcatga tcggcgcagg aattgttcac
     4441 ccgccgttca cttgttttga aaaggcgatc ctcaactggc gtgaccacta ctgccaataa
     4501 caataaccgg ggctaacgca gttagcccca cagtagcgga aaacgttgtt taccttcagg
     4561 gctgggcgat ctccatgatc gcaaacgatg cttaacctgt taaggagatt ccatgaacag
     4621 cataaaacta gaatggaaac gtggtgactg ggcggcttac tttggactga tgaccaacaa
     4681 tctgactaac ctgctgacaa tgatgggtct gctaattttt gtcgtcggaa tccctactga
     4741 aattgtctat ggacgcattg cgcctgcctt tggtcttgca gtgctggcgg ccagtgtctg
     4801 ctactcctgg tttggtctac agatggtgaa gaaaaccggt cgtagcgatg taacggcgct
     4861 gccgtctgga ccgagcgcac cctcaatctt taccgtgaca ttcctggtgc tgatgccggt
     4921 ttatcagcaa accaaagatg ccaactttgc tattcagata ggattggtgt ggtgctttgt
     4981 cgaagcgttg atcctggtcg gtggatcctt cctgggtgag actatccgca agatgatccc
     5041 gcgtacagtg ctgctttctt gtctgtccgg actggggtta ttactgctgg caatgaaccc
     5101 gatgctgcaa gcgttcgaag cgccgacagt ttctttcatc gtcctgttat tgatcttcat
     5161 taactggttc ggtaagaagc cgatttttcc ccatattcct accggtttgc tgttgctgat
     5221 tgctggtacg tccctagcgt ggatatccgg tttacaaagc ccggaagaca ttaaagcgtc
     5281 gatgtcttcc ttcggcttca acccgccgga aattcatgta aggagcttcc tgcaaggttt
     5341 gccgcacgct ctgccatatc tggcttctgc agttcctttg ggcctggcta actacatttt
     5401 tgacctcgag aacattgaaa gtgcgcacgc ggctggtgat gaatacaaca cccgtaaggt
     5461 catgatggcg aacggctttg cctccatgct cggttgcatg ctgggtaatc cgttcccggt
     5521 aaccgtatat gttgggcatg caggctggaa agctatgggc gctagtatct gctataccct
     5581 cgcatcaggc atcactatgt ttctggtgcc attgtttggg ttgggcgcgt ttatgctcgc
     5641 cattattccc atgactgcta tagtgccaat tctggttttt atcggtgtgg taaccgccaa
     5701 tcaggtggtg cgagaaactc ctaaagtcga ggttcccgtc attttcatct gtctgttccc
     5761 atgggtcgcc aactgggcat tgaccatcgt taacagcgtg atgggcgcag ccggaacatc
     5821 cgctgagaaa cttggtagcg acctgttgca cagcaaaggc gtctattacg atggactgat
     5881 gcatctggga agcggtgcac cgctggccag catgctgtgg ggatgcattg ctatctttgc
     5941 cattatgaat aagccgctgc gcggggcgat agctgcatcc tttggagcac tgttatcgct
     6001 gtttggcgtg attcactccc cagcggttgg ctttgcggaa ggcagctcga ttgtgtttgt
     6061 tgtggcctac ctgatgatgc ttgctatgtt tgccctcaag catgttattg atagccgtga
     6121 aacagtggct gcgtctgagc aggaagagac taaaacgacc tgaatagcct gcaaacggaa
     6181 gataattcta tgaaagaact tgtggtcgtt gccattggtg gcaacagcat tatcaaagat
     6241 aacgccagcc agtcgattga gcatcaggcg gaggcggtga aggccgtcgc cgatacggtt
     6301 ctggaaatgc tggcttccga ttacgacatt gtgctgaccc acggcaacgg gccgcaggtc
     6361 ggtctggatt tacgccgcgc ggagatagcc cacgagcgcg aagggctccc cttaacgccg
     6421 ctggcgaact gtgtggcgga tacgcaaggc ggcattggct atctgatcca gcaggcgctg
     6481 aacaaccggc tggcgcctca cggcgagaaa aaagccgtca ccgtggtgac tcaggtggaa
     6541 gtggataaaa acgatccggg gtttgtccat cccaccaagc ccatcggtgc attctttagt
     6601 gaaaaccagc gtgatgaact acaaaaggca aaccctgact ggcgttttgt tgaagatgcc
     6661 ggacggggct atcgccgcgt ggtcgcctcg ccggaaccga aacgtattgt cgaagcacct
     6721 gccattaaga ctctgatcca gcaaggcttt gtggtgattg gcgcgggcgg cggtggcatt
     6781 ccggtggtgc gtactgacgc aggggattac caaagcgtgg acgcggttat cgacaaagat
     6841 ctctctagcg cgctactggc tcgcgaaatt cacgccgaca ttctggtgat caccaccggc
     6901 gtggaaaaag tgagtattca ttttgggaaa cccaaccagc atgcactgga tacagtggat
     6961 gtcctaacga tggcccgtta catgcaggag ggtcacttcc cgcctgggag tatgttaccg
     7021 aagattcttg ccagtctgga gttcctggaa cgtggaggta aaagagtgat tatcaccacg
     7081 cctgaatgcc tgtccgcggc actgcgtggc gaaacaggca cccatattat ccactcccaa
     7141 gaggagacgt aaattaagga aagcgaccgc cgccgtgaat ttttaatgtt cattgcagat
     7201 tatcgtgggc tggaaatgat aacggttggg gagttattgt ttagttgcta aaacagaacg
     7261 ataaccaacc ccaaccgttc tcaatttcga atactatctt ttccatttat ctctggatag
     7321 gctttgacgt cgtccctact gagtaaaatc cccctcatca tccacctaaa accgattgaa
     7381 aacggtcatt actcttgtgg caaacaggct aaagccgttc acgatgcttc acttcgcacc
     7441 ttatcgtaat gaacaatact ggtatttcca gttcagttac gcctacccgt ctaccgcatt
     7501 ctttgcccta actacggcat agttcgggat tatatcagct ggctaaagcc ttatgctcat
     7561 cagaccacat gactcattga gcatgttgag gcgttactca aaatcttgcc gattaaacat
     7621 atttcgtaat ttcttaccct gcactagcat atcatcaata ctatcgacaa acgtcagttg
     7681 caaatagagg tcacagggtc tgattgatgt actgccaagg ccgggtcatg aaagagtttg
     7741 ccctgttcaa agctcatcgt tacgcaccgt cgtagccgat gcggatactc atcaatcctc
     7801 tggataggtg aggtgcgtag tagagaagct cggtcgtttt tcgttaatct gaatcagtac
     7861 tgtcaacata ttcaagtcgt taatatggat atgaatacct cctttgacct agaggtacag
     7921 atgcactacc ccccaagctg tcaacgacgg atgaaaagtg gctcttccgc aacctggatg
     7981 gagattttca tgccagtgga ctcatgaccc tctgcctgag tagttcaaat ccggctcgac
     8041 catacatctg gcctttcagc atttttagcc gatttacatg actttcgaca ccccccatta
     8101 ttccagcgac tgctgattgc ctcacatata gccgcagcat cagctgccat ccttgcagcc
     8161 acgcgccgaa gttaccactt tctcgttgct cttttaacca ctccctccat gggtccagaa
     8221 gtcctcgctc tggaggccgg gttgacattt cagggaatgt tcctgaacac acccagagac
     8281 tgactgtcac acgtgataag cctgtaatac gggataagcc tgtaatacgg gatatttccc
     8341 ctgaacccac atcccttgtt atgtagtgcc ataacctctg tccaacgctg atgccgtttt
     8401 tggcgggttt gctgtttaag gcgttccgga cgacggagcg atgctacagg cacagacagt
     8461 actggcttag gtgatttctt tggtgacaac ccactggcaa caagacgtat cagaggcatc
     8521 tgtctgtaca tcattcgttc aggcgcatcg ccaatatttt ttagcaggtg ccatcgatca
     8581 gctatctgtc tggcctgagg tgcaccttca cgtgctgctg tcgcatagac tccgccacgg
     8641 tcacgtgaga caacctgtat ttccggatat tttctgaacc aggtcgccag cgtactctga
     8701 tcgcaatcgg gaagcaggac gaggggacgg tgagtatcaa gattgacgat taacgtgccg
     8761 taatggtggc cccgatgcca cgcccactca tcaataccga catgaggcgc gcccgactgt
     8821 ttcgtctccg gggtattaat aaccctgcga agaagagtat cagcactgca acggagcccc
     8881 actgccgttg ctgcctgttt cccggcctca cctccggcta ttaatcccag ttgatgttgt
     8941 aaattttgta acacctgtga agaatgctgg gagtgaaccg gcgaaggggg caagcgaccc
     9001 ggcaaaaatt ttacgtgaac aagccgggtt acggcagt
//
LOCUS       DAJOWB010000162         9027 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJOWB010000162 DAJOWB010000000
VERSION     DAJOWB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.162"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1144
                     /locus_tag="OHO85_004263"
     CDS             <1..1144
                     /locus_tag="OHO85_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCQ2673543.1"
                     /translation="VDGLSIIARNFFTLLSGNFKPDWRWDVSKETFTKEKFDSYVKPV
                     FSKIDFYKQCGVINPQNANTAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLK
                     HEEMASSAYKHKNNKVLYDLLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFST
                     IFGDKYILTLLNSMRNMEGNKDSRINKNVVVLAGNHEINFNGNYTARLANHKLSAGDT
                     YNLIKTLDVCNYDSERQVLTSHHGIIRDEEKKCYCLGALQVPFNQMKNPTDPEELANI
                     FNKKHKEHMDDRFIHLIRSNAIASTPVYDNYFNNTTAFRPKPEDIFKCGQTLKKTKQK
                     YGHYGLGVDQHQKIDNYTMGLNSWKIAPNERGDKKGVPGLSCFQPH"
     gene            1406..3028
                     /locus_tag="OHO85_004264"
     CDS             1406..3028
                     /locus_tag="OHO85_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCQ2673544.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMRAHILRR
                     DNNYQFIVTINSKNYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPVWDWK
                     ISQFNVSDIKFETMINPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLRIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGVRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYAGIKDYPVCFYDPKYKIMANHHGITFDEQRKRYIIGPIT
                     VSIDEMTNALDPVELAAIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            3394..4461
                     /locus_tag="OHO85_004265"
     CDS             3394..4461
                     /locus_tag="OHO85_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCQ2673545.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAHRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            4458..7193
                     /gene="rbbA"
                     /locus_tag="OHO85_004266"
     CDS             4458..7193
                     /gene="rbbA"
                     /locus_tag="OHO85_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCQ2673546.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            7193..8317
                     /locus_tag="OHO85_004267"
     CDS             7193..8317
                     /locus_tag="OHO85_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2673547.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            8376..8651
                     /locus_tag="OHO85_004268"
                     /pseudo
     CDS             8376..8651
                     /locus_tag="OHO85_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            8648..9007
                     /locus_tag="OHO85_004269"
     CDS             8648..9007
                     /locus_tag="OHO85_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCQ2673548.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
BASE COUNT         2364 a         2039 c         2324 g         2300 t
ORIGIN      
        1 agttgatggg ctttctatca tagcgagaaa tttctttact cttcttagtg gtaatttcaa
       61 acctgactgg cgttgggatg tctctaaaga gacgtttact aaggaaaagt ttgatagcta
      121 tgttaaacct gtgttttcaa aaatagactt ctataaacag tgtggtgtga ttaacccaca
      181 aaatgccaac acggcttatt ttggcgatac agatggaaga gtaggcgctg tactttatgc
      241 attgctcgtt tcagggcata taggcatcag ggaaaagggt tggagtttat tatgtgaatt
      301 attaaaacac gaagagatgg cttcatctgc atacaaacat aaaaataata aagtgctcta
      361 cgatttatta aatacgagag acatgatctt gaatgagttg catcaacatg tttttttaaa
      421 ggacgatgcg ataacccctt gtatcttcct tggggatcat acaggcgata gatttagtac
      481 tatttttggt gataaatata tccttacttt gctaaattct atgcgaaata tggagggtaa
      541 taaagatagc cgtattaata aaaatgtcgt cgttctggct ggaaatcatg aaatcaattt
      601 taatggtaac tatactgcac gactagcaaa tcataaactc tctgccggtg atacgtataa
      661 cttaattaaa acgctcgatg tttgtaatta tgactcagaa aggcaagttt taaccagtca
      721 tcacggaata ataagagatg aagaaaaaaa atgttattgt ctcggtgctt tgcaggttcc
      781 atttaatcag atgaaaaatc ctactgatcc tgaggaatta gccaatattt ttaataaaaa
      841 acataaagaa cacatggatg atcgttttat ccatctaatt agaagcaatg ctatagcatc
      901 cacacctgta tatgacaatt attttaataa cactactgct ttcagaccaa aacctgaaga
      961 tatcttcaaa tgtggacaga cgctcaaaaa aactaaacag aaatatggcc attatggatt
     1021 aggggtagat cagcatcaaa aaattgataa ttataccatg gggctaaatt cctggaagat
     1081 agctcctaac gaacgtggtg ataaaaaagg tgtcccaggg cttagttgtt ttcaaccaca
     1141 ttaaaactcc atgagtgctg tggtatatct ttttaatcct gccagtattt cataggtttt
     1201 cttaatcgat tgtagaggca tgttctgatg acgcctctac aatctttagg gactttctct
     1261 gctatgagat aagaaatttc atattatgct ttaaggctga atgatttgtg caaaatataa
     1321 ttatttttcc atcgaacatg aggtgtatgt cataagatta aatgttaact atgcattcct
     1381 cgcggtgaat tatatgaggt aaaagatgaa aattggaact gtggcaggca ctaacgacag
     1441 tacaacgaca atcgcaacaa atgatatggt gcaagagcat gttactaact tcactaaaga
     1501 actgtttggg tatattgcaa atggtatagg tgatgatata tcaagtattg ccaggacaat
     1561 gctcggtgaa gttgtggaaa aaattgatga ttggcaaata gaacgtttcc aacaatctat
     1621 tcgagatgac aaaatatcat ttaccataca aacgaatcat tcagaaaaat atagtatgct
     1681 atcggacatg cgcgctcata ttctacgtag ggataataat tatcagttta ttgtgacaat
     1741 taatagtaaa aattacggct gctatcttga taataccgat attaattggt gcagcattgt
     1801 ttatttactt aataatatga cagtaaatga tgatgctaat gatgttgcag ttactgagtc
     1861 gtacaaaccc gtctgggact ggaaaatatc gcaattcaat gtatctgata ttaaattcga
     1921 aacaatgata aacccccagt tcgctgacag aacctatttc tccaattgtt cacctgttga
     1981 tcccacaagt accagaccaa cctattttgg cgataccgat ggctcagtcg gtgcagtttt
     2041 gtacgcgctt tttgcaacgg gacatttgag aatcatggct gaaggagaaa atttccttag
     2101 ccagttgctt aatatagaag atgaagttct caacgtattg ctaagggaga actttaatga
     2161 gcaacttgac actaatgtta atacaataat cagtattctg aacagaagag atatagtttt
     2221 agaaagtctt cagccttatt tagttattaa taaagacgct gtgacgccat gtacgtttct
     2281 tggcgaccaa accggcgttc gatttagtaa tatctgtggc gatcaattta tcatcgattt
     2341 gttaaaacgc ataatgagta tcaatgaaaa tgttcatgta ttagctggaa atcatgaaac
     2401 gaattgcaat ggtaactata tgcagaactt cacgcgtatg aaaccacttg atgaggatac
     2461 ctacgctggg ataaaagatt atcctgtatg tttctatgac cccaaataca aaataatggc
     2521 aaatcatcat gggataactt ttgatgagca gcgaaaacgt tacattatag gaccgataac
     2581 agtctctatt gatgaaatga ccaatgctct cgatccggtc gaattagcgg caattatcaa
     2641 taaaaagcat catgctataa ttaatggcaa aaagtttaaa actagccggg ctatctcttg
     2701 tcgatccttt aatcgttatt tttctgtttc gacagattac agacctaaac ttgaagcact
     2761 attagcatgt tcacaaatgt taggtataaa tcaagttgtt gcacataacg gaaatggtgg
     2821 aagagaacgc ataggcgaaa cggggacagt tttaggtctt aacgcccgtg acagtaaaca
     2881 tgctggaaga atgttcagca tgcataattg ccaaattaac cctggtgcag ggcctgagat
     2941 aaccactccc tggaagtctt accagcatga aaaaaacaga aatggactta tgcctttgat
     3001 tagaaggcgc acaatgttac agctctgaac catagacaac atgaatatta atgttactat
     3061 ctggttgaga ctgttgataa aacgtaaaaa ggaatgctct gttcctgcaa ctgaacaggc
     3121 gtgaaacgct atccaacagg atggataccg ttttgtcagc cagttttata gatcacactt
     3181 atgagtatgc gtgtttcatt tacggccagg aatgctgatg atgacaacat tactacccgt
     3241 tttcactaag ccttccccgt tggcgctcaa tgctctgcgc gctggtcgta tttgccgttt
     3301 ccttcttatc ccggatggaa gaatccgctg aacgtcgttc ggcggcagtt gtaacacgcg
     3361 tcgtttgctt acagggatta tggcgggtcg attatggata agagtaagcg ccatctggcg
     3421 tggtgggttg tcgggttact ggcggtggcg gctatcgtgg cgtggtggct gttgcgcccg
     3481 gcaggtgtgc cggaaggctt tgctgtcagc aatgggcgca ttgaagcgac ggaagtggat
     3541 atcgccagca aaattgccgg gcgtattgac accattctgg tgaaagaagg ccagtttgtt
     3601 cgagaaggtg aagtgctggc gaagatggat actcgcgtgt tgcaggaaca gcgactggaa
     3661 gccatcgcgc aaatcaaaga agcacaaagc gccgttgctg ccgcgcaggc tttgctggag
     3721 caacgccaaa gtgaaactcg cgccgctcag tcgctggtta atcaacggca agctgaactg
     3781 gactccgtag ccaaacgtca tacccgttcc cgctcgctcg cccatcgtgg ggctatttct
     3841 gcgcaacagt tggatgacga ccgcgccgcc gctgagagcg cccgagctgc gctggaatcg
     3901 gcaaaagctc aggtttcggc ttctaaagcc gctatagaag cggcacgcac caatatcatt
     3961 caggcgcaaa ctcgcgtcga agccgcacaa gccactgaac ggcgcattgc cgcagatatc
     4021 gatgacagcg aactgaaagc cccgcgtgac ggacgcgtgc agtatcgggt tgccgagcca
     4081 ggcgaagtgc tggcggcagg cggtcgggtg ctgaatatgg tcgatctcag cgacgtctat
     4141 atgactttct tcctgccaac cgaacaggcg ggcacgctga aactgggcgg tgaagcccgg
     4201 ctgatcctcg atgccgcgcc agatctgcgt attcctgcaa ccatcagttt tgtcgccagt
     4261 gtcgcccagt tcacgccaaa aaccgtcgaa accagcgatg aacggctgaa actgatgttc
     4321 cgcgtcaaag cgcgtatccc accggaatta ctccagcagc atctggaata tgtcaaaacc
     4381 ggtttaccgg gcgtagcgtg ggtgcgggtg aatgaagaac ttccgtggcc tgacgacctc
     4441 gtggtgaggt tgccgcaatg acgcatctgg aactggttcc cgtcccgcct gtcgcgcaac
     4501 tggcgggcgt gagccagcat tatggaaaaa ccgttgcgct gaacaatatc acactcgata
     4561 ttccggcccg ttgcatggtc gggctgattg gcccggacgg cgtcgggaag tcgagcttgt
     4621 tgtcgttgat ttccggtgcc cgcgtcattg aacagggcaa tgtgatggtg ctgggcggcg
     4681 atatgcgcga cccgaagcat cgccgcgacg tctgcccgcg catcgcctgg atgccgcagg
     4741 ggctgggcaa aaatctctac cacaccttat cggtgtatga aaatgtcgat tttttcgctc
     4801 gcttgttcgg tcacgataaa gcggagcggg aagtccgaat taatgagctg ctgaccagca
     4861 ccgggttagc accgtttcgc gatcgtccgg caggtaaact ctccggcggg atgaagcaaa
     4921 aactggggct gtgctgcgcg ttaatccacg acccggaact gttgatcctt gatgagccaa
     4981 caacgggggt tgacccgctc tcccgcgccc agttctggga tctgatcgac agtattcgcc
     5041 agcggcagag caatatgagc gtgctggtcg ccaccgccta tatggaagag gccgaacgct
     5101 tcgactggct ggtagcgatg aatgccggag aagtgctggc aactggcagc gccgaagagc
     5161 tgcggcagca aacgcaaagc gccacgctgg aagaggcgtt tataaatctg ttaccgcaag
     5221 cgcaacgcca ggcgcatcag gcggtagtga tcccaccgta tcaacctgaa aatgcagaga
     5281 ttgccatcga agcgcgcgat ctgaccatgc gttttggttc cttcgttgcc gttgatcacg
     5341 tcaatttccg cattccacgc ggggagattt ttggttttct tggatcgaac ggctgcggta
     5401 aatccaccac catgaaaatg ctcaccggac tgctgcccgc cagcgaaggt gaggcgtggc
     5461 tgtttgggca accggttgat ccaaaagata tcgatacccg ccgtcgggtg ggctatatgt
     5521 cgcaggcgtt ttcgctctat aacgaactca ccgtgcggca aaaccttgag ttacatgccc
     5581 gtttgtttca catcccggaa gcggaaattc ccgcgcgagt ggctgaaatg agcgagcgtt
     5641 ttaagctcaa cgacgttgaa gatgttctgc cggagtcatt gccgctcggc attcgccagc
     5701 ggctttcgct ggcggtggcg gtgattcatc gcccggagat gttaatcctc gatgaaccta
     5761 cttctggcgt cgatccggtg gcgagggata tgttctggca gttgatggtc gatctctcgc
     5821 gccaggacaa agtaaccatt tttatctcca cccactttat gaacgaagcg gaacgttgcg
     5881 accgcatctc actgatgcac gccggaaaag tgctcgccag cggtacaccg caggaactgg
     5941 ttgagaaacg cggagccgcc agtctggaag aggcatttat cgcctatttg caggaagcgg
     6001 caggacagag caacgaagcc gaagcgccgc ccgtgataca cgacaccacc cacgcgccgc
     6061 gtcagggatt tagcctgcgc cgtctgttta gctacagccg ccgcgaagca ctggaactgc
     6121 gacgcgatcc ggtacgttcg acgctggcgc tgatgggaac agtgatcctg atgctgataa
     6181 tgggttacgg catcagtatg gatgtggaaa acctgcgctt tgcggtgctc gaccgcgacc
     6241 agaccgtcag tagccaggcg tggacgctca atctctccgg ttcccgttac tttatcgaac
     6301 agccgccgct caccagttat gacgagcttg atcgtcggat gcgtgcgggc gatataacgg
     6361 tggcaattga gatccctccc aatttcgggc gcgatatcgc gcgtggtacg cctgtggaac
     6421 tcggcgtctg gatcgacgga gcgatgccga gccgtgccga aacggtaaaa ggttacgtgc
     6481 aggcaatgca ccagagctgg ctacaggatg tggcgagccg acaatccaca cccgccagcc
     6541 aaagcgggct gatgaatatt gagacgcgct atcgctataa cccggacgta aaaagcctgc
     6601 cagcgattgt tccggcggtg atcccgcttc tgctgatgat gatcccgtca atgctaagcg
     6661 cccttagcgt ggtgcgggaa aaagagcttg ggtcgattat caacctttac gtgaccccca
     6721 ccacgcgtag tgaatttttg cttggtaaac agttgccata catcgcgctg gggatgctga
     6781 actttttcct gctctgcggc ctgtcggtgt ttgtgtttgg cgtaccgcat aaaggcagtt
     6841 tcctgacgct caccctggcg gcgctgctgt atatcatcat tgccaccgga atggggctgc
     6901 tgatctccac ctttatgaaa agccagattg ccgccatttt cggaacggcg attatcacgt
     6961 tgatcccggc gacacagttt tccgggatga tcgatccggt agcttcgctg gaagggcctg
     7021 gacgttggat cggcgaggtt tacccgacca gtcattttct gactatcgcc cgcggaacgt
     7081 tctcgaaagc gctggatctg actgatttgt ggcaactttt tatcccgttg ctgatagcca
     7141 tcccgctggt gatgggctta agtatcctgc tgctgaaaaa acaggaggga tgatgcgcca
     7201 tttacgcaat atttttaatc tgggtatcaa agagttgcgc agtctgctcg gtgataaagc
     7261 gatgctgacg ctgattgtct tctcgtttac ggtgtcggtg tattcgtcag cgaccgttac
     7321 gccaggatcg ttgaacctcg cgccgatcgc cattgccgat atggatcaat cgcagttatc
     7381 gaaccggatc gttaacagct tctatcgccc gtggtttttg ccaccggaga tgatcaccgc
     7441 cgatgagatg gatgccggac tggacgccgg gcgttatacc tttgcgataa atattccgcc
     7501 taattttcag cgtgatgtcc tcgccggacg ccagccggat attcaggtga acgtcgatgc
     7561 cacgcgcatg agccaggcat ttaccggcaa tgggtatatc cagaatatta tcaacggtga
     7621 agtgaacagc tttgtcgcgc gctaccgtga taacagcgaa ccgttggtat cgctggaaac
     7681 ccggatgcgc tttaacccga accttgatcc cgcgtggttt ggcggggtga tggcaatcat
     7741 caacaacatt accatgctgg cgattgtatt gaccggatcg gcgctgatcc gcgagcgtga
     7801 acacggcacg gtggaacact tgttggtgat gccgataacg ccgtttgaga tcatgatggc
     7861 aaaggtctgg tcgatggggc tggtggtgct ggtggtgtcg ggattatcac tggtgctgat
     7921 ggtgaaaggc gtgctgggtg taccgattga aggctcgatc ccgctgttta tgctgggcgt
     7981 ggcgctcagt ctgtttgcca ccacgtcaat cggcattttt atggggacga tagcgcgttc
     8041 aatgccgcaa ctggggctgc tggtgattct ggtgctgctg ccgctgcaaa tgctttccgg
     8101 tggatccacg ccgcgcgaaa gtatgccgca gatggtgcag gacattatgc tgaccatgcc
     8161 gacgacacac tttgttagcc tcgcgcaggc cattctctac cggggtgccg gattcgaaat
     8221 cgtctggccg cagttcctga cgctgatggc aattggcggc gcgtttttca ccatcgcgct
     8281 gctgcgattc aggaagacga ttgggacaat ggcgtaagga agggaaactt gacttcatac
     8341 tgtgatgtca taaagtgaag tcataaggga atgtcatgta aacacaggta caggctttac
     8401 gaaagaaaca aaaaaacaca ctggatcagc tatttaaatc acctttacct caaggaatca
     8461 aatggactga tatagaatct ctggttaaag cgttaggcgg agaaattaag gaaggtaggg
     8521 ggtcgcgctg taagttcata ctaaatatga gcgttgcgtg tttccatcgg cctcatccgt
     8581 cgccagatac cgataaaggc gctgtagaaa gcgtgcgtga ctggttacta agtatagggg
     8641 taaaaccatg atcaaactga agacgccaaa ctctatggag attgccgggc aacctgctgt
     8701 tatcacttac gtgccagaac tgaatgcatt tcgtggcaaa ttcctgggct tgtcaggtta
     8761 ttgtgatttt gtctctgaca gcattcaggg gctgcaaaaa gaaggggaac tttccctgcg
     8821 agagtatctt gaggattgta aagctgcggg tattgagccg tatgcgcgta cagaaaagat
     8881 taagaccttt acgttacgct atccagaatc gctgagtgaa cgccttaata atgcggctgc
     8941 gcagcaacaa gtctctgtta atacctatat tattgaaaca ctgaatgagc gtttgaatca
     9001 tttataaatt ttagcggcgt acattac
//
LOCUS       DAJOWB010000163         8752 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJOWB010000163 DAJOWB010000000
VERSION     DAJOWB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.163"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..952
                     /locus_tag="OHO85_004270"
     CDS             <1..952
                     /locus_tag="OHO85_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HCQ2673549.1"
                     /translation="KLIIFGNGLGMALDPAHFSLDRALAEIWHRSNFLNDVHKQLIER
                     CLQRQGPPAGEHELDTLHQAITYCKALAQIGEGNEHWLTDDGLNFPEITATYIHKVAT
                     SLHNYGEGLPQRFENALVEFVKNTRTHIATLNYDKLLYNSFIDNDIFSGYDGYLVDGM
                     LGHGFSAEALERKYNRRFGYYLHLHGSPLFVNQGETVLKLSRSQLTVDRDEPSEHIVL
                     THIKRKPSVIAASNVLSTYWDYLQFALFESEEIILFGYSGLDEHLNLLIRPYLTSKHL
                     RVIEWSGAGEQNDREAYWQYLLGREVTVTRLDNITDFINW"
     gene            1413..1568
                     /gene="hokD"
                     /locus_tag="OHO85_004271"
     CDS             1413..1568
                     /gene="hokD"
                     /locus_tag="OHO85_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCQ2673550.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1785..2036
                     /locus_tag="OHO85_004272"
     CDS             1785..2036
                     /locus_tag="OHO85_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673551.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDGRVTDGFILREGEFVTSLQALKEIGIKAGFSAFAEE"
     gene            2103..2381
                     /locus_tag="OHO85_004273"
     CDS             2103..2381
                     /locus_tag="OHO85_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673552.1"
                     /translation="MARNVKYYNSDNSPVLACTHERYSHAFKSEWFQHPPCTEERAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            2383..3432
                     /locus_tag="OHO85_004274"
     CDS             2383..3432
                     /locus_tag="OHO85_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCQ2673553.1"
                     /translation="MRVLLRPVLVPELGLVVVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLCDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            3445..3819
                     /locus_tag="OHO85_004275"
     CDS             3445..3819
                     /locus_tag="OHO85_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCQ2673554.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRMGVKIYPIMHEEQVKK"
     gene            3816..4637
                     /locus_tag="OHO85_004276"
     CDS             3816..4637
                     /locus_tag="OHO85_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCQ2673555.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            4781..4856
                     /locus_tag="OHO85_004277"
     tRNA            4781..4856
                     /locus_tag="OHO85_004277"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4814..4816,aa:Ile,seq:cat)
     gene            4957..5033
                     /locus_tag="OHO85_004278"
     tRNA            4957..5033
                     /locus_tag="OHO85_004278"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4991..4993,aa:Arg,seq:tct)
     gene            5533..5664
                     /locus_tag="OHO85_004279"
     CDS             5533..5664
                     /locus_tag="OHO85_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCQ2673556.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            6031..6459
                     /gene="tolA"
                     /locus_tag="OHO85_004280"
     CDS             6031..6459
                     /gene="tolA"
                     /locus_tag="OHO85_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCQ2673557.1"
                     /translation="MGFRSASILTLIISGIIIGCSDAVSTNYHDRTSYYSDKAIETQY
                     VSSSERTSDVSEDIRLYAHQIKSAIEKQFGDASKYSGKECTLRMHMAPNGLLLEVKRE
                     SGDLDLCREAMNAIKNADIPAPPSPEVYKVFQNGVLDFKP"
     gene            6631..7005
                     /locus_tag="OHO85_004281"
     CDS             6631..7005
                     /locus_tag="OHO85_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tolA family protein"
                     /protein_id="HCQ2673558.1"
                     /translation="MLTFWVGFVAGCTPLHPSDCHKTTATGSCSSGRWDDQDEWGAQA
                     RGIRAAINAKLDEPHNWKGKKCRLHMEFSQDGTALKISTSNGDKAYCEAIKSAAHKAK
                     FPAFNNPEVYRDFQKSGFDMRG"
     gene            7257..7472
                     /gene="essD"
                     /locus_tag="OHO85_004282"
     CDS             7257..7472
                     /gene="essD"
                     /locus_tag="OHO85_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCQ2673559.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            7477..7788
                     /locus_tag="OHO85_004283"
     CDS             7477..7788
                     /locus_tag="OHO85_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HCQ2673560.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFGLDVAMDR
                     VEGAALEFYEAAARMSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     L"
     gene            7785..8318
                     /locus_tag="OHO85_004284"
     CDS             7785..8318
                     /locus_tag="OHO85_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCQ2673561.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKNVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            8315..>8752
                     /locus_tag="OHO85_004285"
     CDS             8315..>8752
                     /locus_tag="OHO85_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HCQ2673562.1"
                     /translation="MNQIFTVILLVLVGFVVGNVWSDRGWQKKWAERDAAELSQEVNV
                     QFAARIIEQGRSISRDEAVKDAQQKAAEISARAADLSDSVNQLRAEATKYAIRLDAAQ
                     HTANLAAAVRGKTTKAAEGMLTNMLGDIAAEAQLYAEIADKRYI"
BASE COUNT         2356 a         1824 c         2276 g         2296 t
ORIGIN      
        1 aaaattaata atattcggca acggtcttgg tatggcttta gatcctgctc atttttcctt
       61 agacagggcg ttggcagaaa tttggcatcg atccaatttc ttaaacgatg tccataagca
      121 acttatcgaa cgttgtcttc agcggcaagg tccaccggca ggggaacatg agttggatac
      181 ccttcaccaa gctatcacat attgtaaggc tcttgcgcaa attggagaag gaaacgagca
      241 ttggcttact gacgatggac taaacttccc cgaaatcacc gccacttata tccacaaagt
      301 tgcaactagt ttgcataatt acggtgaagg gttgccgcaa agattcgaaa atgcgctagt
      361 ggaatttgta aaaaatacac gcactcatat tgcgacatta aactatgaca aattattata
      421 caactcattc atagataatg atatattcag cgggtatgat gggtatctgg ttgatgggat
      481 gttaggccac ggattttccg ccgaagcgct cgaacggaaa tataatcgta ggtttggtta
      541 ttatttgcat ttgcatggct caccactatt tgttaatcag ggtgagaccg tcttaaaact
      601 ttcacgttct caacttacgg ttgacaggga tgagcctagc gaacatatag tgttaacgca
      661 tataaaaagg aaaccgtcag ttatagcagc atctaatgtt ctttctacgt actgggatta
      721 tttgcagttt gcattatttg aatcagaaga aataatactg tttggttatt ctggcctcga
      781 tgaacacctt aacctgctta ttcggcctta cctaacctct aagcatttaa gagtaattga
      841 gtggagcggt gcaggtgaac aaaatgaccg tgaggcatac tggcaatatt tgttaggacg
      901 ggaagttact gtaaccaggt tggataacat cactgacttc atcaattggt agcacaatgc
      961 gaagttagta tcgagagtat acggttctaa cttcgctgag ctgaaatata cactttacct
     1021 tcatttcatt ctttagatga acacccaata gttgtattga atcattgaag aggcagtctc
     1081 atatttatca gtattggtat acgtccaata caggaggtta tcgtgttggc tctgacgtgt
     1141 gtgttggcta ttgtggctgt gctggtaatt tattgtcttg ttgttgcttt catggagcga
     1201 ttttctgatt ggcgaggtag agggagttaa gtaaaattgc tgcgggtgct tgaggctatc
     1261 tgcctcgggc atgaacacca acggcagata gagaaaagcc ccagttaaca ttacgcgtcc
     1321 tgcaagacgc ttaacattaa tctgaggcca atttcatgct agacacatgt aggttagcct
     1381 cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg atgttaatcg
     1441 ccctgatcgt catctgttta accgtcatag tgacggcact ggtaacgagg aaagacctct
     1501 gcgaggtacg aatccgaacc ggccagacgg aggtcgctgt cttcacagct tacgaacctg
     1561 aggagtaaga gacccggcga gggagaaatc cctcgccacc tctgatgtgg caggcatcct
     1621 caacgcaccc gcacttaacc cgcttcggcg ggtttttgtt tttattttca acgcgtttga
     1681 agttctggac ggtgccggaa tagaatcaaa aatacttaag tagcgcgcag ggataagagg
     1741 gatggtccct taaaggggag agctaattat ccggaagaat tctgatgatg aacatcgaag
     1801 aactgcgtaa aattttttgt gaagatggcc tctatgctgt gtgcgttgaa aatggaaatc
     1861 ttgttagtca ttaccgcatt atgtgtttgc gaaagaatgg ggctgcgtta attaattttg
     1921 tggatggtcg agtgacagac ggatttatct tgcgcgaagg tgagtttgtc acttcattac
     1981 aggcactgaa agagatcgga ataaaagcag gcttttcagc ttttgcagaa gaataaactc
     2041 atctacaatc ttgcgcgggg ctgaactccc gctgagtaac accgtgccac cggagaaaac
     2101 cgatggcacg caacgtaaaa tattacaatt ctgataattc gcccgttctt gcctgcacgc
     2161 acgagcggta ttctcacgca ttcaagtctg aatggttcca gcaccctcca tgcactgaag
     2221 agcgggctga atggataatt cagtgttacc gcaggcgcgg atacgaggtt aagaaagccc
     2281 ttagtctcga ctaccgtcac tggataatct cagtcagatt gccttactcc gaacgcccac
     2341 cgcgtccgtc ccgtacattc cagcaacgca tctggaggta acgtgcgggt attacttcga
     2401 cctgttctgg taccggaact cgggctggtg gtcgttaagc cgggccgtga atccatgccg
     2461 gtattccaca atacccgggt actggtggag ccggaaccga aaagcatgcg taatctgccg
     2521 tccggggtcg ttcctgccgt tcgccagccg ctggtggaag acaaaacatt gctgccgttt
     2581 ttcagtaacg cacgggtaat tcgtgctgct ggtggtgctg gtgcattgtc tgactggctg
     2641 ttgcgccata ttaaatcctg ccagtggcca cacggcgatt atcatcacag cgaaaccgtt
     2701 attcaccgtt atggtaccgg cgcaatggtg ttgtgctggc actgcgacaa ccagctgtgt
     2761 gaccagacat ccgaatcact cgagcaactt gctcatcaaa acctgtcagc atggatgatt
     2821 gacgtcatcg gtcacgcaat aagcggtacg caggagcgtg aattatctct ggctgaatta
     2881 tcctggtggg cggtccgcaa tcaggtggcg gacgcgctac cggaagcggt attacgtcgt
     2941 tcgctggggt tgcgtgcgga aaaaatccgc tcaatgtacc gtgaaagcga catcgtaccg
     3001 ggagagcaga ccgccaccag catactgaaa cagcgcacaa aaaatcttgc gccgctgcct
     3061 cacgcccacc agcaacagaa cccaccacag gaaaagacgg tggtcagcat tgccgttgat
     3121 cctgagtctc cggaatcttt tatgaaacga cctaaacgtc gccgctgggt taacgagaaa
     3181 tacacacgct gggtgaagac acagccgtgt gcgtgttgtg gtaagccagc cgacgatccc
     3241 catcacctga ttggtcatgg tcagggcgga atggggacaa aatctcacga tattttcacg
     3301 ctaccgctgt gtcgggagca tcacaacgag cttcatgcgg atccgctggc gttcgaagaa
     3361 aagcatggtt ctcaggttga tttaattttt cgttttcttg atcacgcctt tgcaactggc
     3421 gtgcttgggt aaaagaggtg actgatgctc atagatttgg ttttacctta cccgccgacg
     3481 gtgaacactt actggcgacg ccgtggcagc acatatttta tctcggagga gggaaagcgt
     3541 tatcgccggg ctgtggcgct tattgttcgc cagcagcggc tgaaattaag cctgtccgga
     3601 aggctggcga taaaggtgat tgcagagcca ccggataagc gtcgtcgcga cctggacaat
     3661 atcctgaaag caccgctgga tgcgctgacg catgcgggag tgttaatgga cgatgagcag
     3721 tttgatgaaa tcaatatcgt tcgtggtcag ccagtatctg gtggacgtat gggggtgaag
     3781 atttacccca taatgcatga agagcaggtc aaaaaatgaa actggaagat ttaccgaaat
     3841 actactcccc aaaatcccct tgcctgaccg atgcatcggc ttcaacgtca aaagatgcgc
     3901 tgagtatcac tgatgtgatg gccgcgcagg gcatgacaca gaatcgggct gagatggggt
     3961 tttctgcgtt cctggggaaa atgggcatca gtatgaatga cagggcacgg gcaacagaat
     4021 tactggcaga ttatgcactc agtcggtgcg atcgtgtggc ggcgttgaga aaacttccgg
     4081 cagaaataaa accggtagtg atgcgcatta tggcttcgta cgcttttgag gattatgccc
     4141 gcagcgcagc gagtaaaaag cagtgccctt gttgctatgg ggaaaaattt attgaaagcg
     4201 tagtttttac aaacaaggtc cagtatccgg atggtaagcc gccggtatgg gcaaagtgta
     4261 cgaaaggtgt gtatccgtct tactgggaag aatggaaaaa agtcagggag gtggtaaaag
     4321 ttgcctgtcc ggagtgtggc ggaaagggtg aggtttccac cgcctgtaag gattgccgtg
     4381 ggcgtggtgt cgccattcac cgtgaagagt cggtaaaacg tggtatgcct gttatcagag
     4441 actgccagcg ttgtggtggt cgtggctatg aaagactacc atcaacggag gcatttaatg
     4501 ctatatgcga ggtgacaaac cagataacac gcgcgtcatg ggaaaaaaca gttaagaaat
     4561 tctatgatgc gctggtgacc cggtttgata ttgaagaagc atgggctgag cggcagttaa
     4621 aaaaggtaac taggtaacaa ggttgatttt tccggaatct gtggtaaatt catcataatg
     4681 atgggctttt tatgcctgac gttagaagag tttctacaac ccgccgccga gcgggttttt
     4741 tattgcggaa ttaattacgg accgttatta ttctgctccc ggccctttag ctcagtggtg
     4801 agagcgagcg actcataatc gccaggtcgc tggttcaaat ccagcaaggg ccaccatcac
     4861 aaaccgccat tagcttatca ggaagagcag acgacacgat aacagggttg ttggtgcggg
     4921 ggcgggtccc cgatggcggt ccattatcgg tattcagcgt tgttagctca gccggacaga
     4981 gcaattgcct tctaagcaat cggtcactgg ttcgaatcca gtacagcgcg ccatattcat
     5041 tcttccagat tccttccggc agagccttat actggaatat accttgctca ggatattgtt
     5101 gaaaacataa tatgtttgtc aaaaataaaa gttctgttaa gtattgattg agtgtttgtt
     5161 atacggtcta atggtttttt cagcattaaa tatttatcat tcatatggtg tgggtagagt
     5221 gaatattgat gaggcgtcgg ggtgtttcat ccttaggcag cgtattgata tagtcaatgc
     5281 agcacgagca aaggccttca gccgtttgac agttttgttc tgtgctcctg atcgtctttc
     5341 gggaagagac gttattattc tgaatagtga tgctatacag agggtttgcg atgagttcat
     5401 ggttgctaat tcagaattat ttgctcttgt tcaggagtac aacagaatag ccaggacctg
     5461 tggtatggat gaacttcgga ttactcatct ggggtagata catatctgga ttatcacctg
     5521 ttacggtaaa aagtgattgc ttactgtttt tgtgaatggc attgcagcag ccggataatg
     5581 tcagtgctgg ctgacggtgt gctggtggcg ggtgtggtgg ttgttgcttt cccgttgctg
     5641 aaaaagaaaa cgccagactg ttagccgggt atcagttagc gggagaaatt tttaaatact
     5701 tcacaattca ggcggttgac tgttctctgg tttgcgggga gtttgttaaa agaaactggc
     5761 atggtgaatc cccctgtgcg gaggggcaat cagcgagtag gtatatggga taatcgcgga
     5821 ttcaggtgct ggtactgaat tcaccgggag gcacccggca ccatgcactt caatagattc
     5881 tctccacatt atggatattc tttcagaata tcccacgcag actttgtgta aatgttaaca
     5941 aatgtgcgtt ttatttgatc tgattcgctg tttgagcgtc caaaacaacg gtatatataa
     6001 tcctttacta tatgacatat gtgaggaaaa atgggttttc gtagcgcatc aattcttacg
     6061 ttgattatta gtgggattat tatcgggtgc agtgatgctg tatcgacaaa ttatcatgac
     6121 cgtacatcat attactccga taaagcaata gagacacagt atgtgagttc atctgaacgt
     6181 acttctgatg ttagtgagga tatccgtctg tatgcccatc aaatcaagag cgccatcgaa
     6241 aaacagttcg gggatgcgag taagtattca ggaaaagagt gtacactgag aatgcatatg
     6301 gccccgaatg gccttctact ggaggttaaa agagaaagtg gagacctcga tttatgtcgt
     6361 gaagcgatga atgcgataaa gaatgctgat atacctgccc ccccttcgcc ggaagtatat
     6421 aaagtatttc aaaatggggt gctggatttt aaaccctgat atttattgtt ttgtaataaa
     6481 cggtttcggc ttaggtttgt tctgacacaa ctacggcact gagctaaatt tagcggatag
     6541 tcagctctga gccagtggcg gacgtaacaa ctactattgc tgagatttta atggattgag
     6601 gagcaagaag tgggattaaa gaaaatcgtt atgttgactt tttgggtcgg ttttgttgcg
     6661 ggatgcacac ctttacaccc ttcagattgc cacaaaacta ctgctacagg tagttgcagt
     6721 tcaggacgct gggatgatca ggatgaatgg ggggcgcaag cgcggggaat cagagctgca
     6781 attaatgcca aacttgatga gccgcataac tggaaaggga aaaaatgcag gttgcatatg
     6841 gaattctctc aggatggcac ggcgttaaaa atatctacca gtaacggtga taaagcctat
     6901 tgcgaagcga taaagtccgc agctcataaa gccaaatttc cggccttcaa caatccggaa
     6961 gtctacagag attttcagaa atctggcttt gacatgcgag gttagctctt caattactat
     7021 atctcattca tagcaaactg acagatttga tgatgttcta tatacgaaac ctgtgatgtc
     7081 aagtctgagc taatacaaat aaacataata tcagagaaat acattttatt agctcgctac
     7141 ggcgagcttt ttatattgca tcgtctccag catatatatc aattaaggct ctgattgatg
     7201 tgtctggaag cctacacata ataactatgc catccgttcc gtgcggaggt gaggctatga
     7261 aatccatgga caaaatttca acaggcattg cctacggcac ctccgcaggc agtgctggct
     7321 actggttttt acagctgctc gataaagtca cgccctcaca gtgggcagca ataggtgtgc
     7381 tgggtagcct ggtatttggc ctgctgacgt acctgacaaa tctttatttc aagattaaag
     7441 aagacaggcg taaggctgcg agaggagagt aatccaatga ctcaagacta tgaactggtt
     7501 gtgaaaggag tccgtaattt tgagaataaa gttacggtaa ctgtagcctt acaggacaaa
     7561 gaacgctttg acggtgaaat ttttggcctg gatgtcgcca tggaccgtgt tgaaggagct
     7621 gcgctggagt tttatgaggc agcagccaga atgagcgtcc ggcaagtctt cctggaagta
     7681 gcagaaaaat tgtcagaaaa agttgagtct tatctgcagc atcagtactc ctttaagatt
     7741 gaaaatcctg ccaataagca cgagcgtcct catcataaat atctatgaac acaaaaatca
     7801 gatacggcct gtcggctgcc gttctggcgc tgattggtgc tggcgcatct gctcctcaga
     7861 tacttgacca gtttctggac gaaaaagaag gtaaccacac aatggcatac cgcgatggtt
     7921 ctggcatatg gaccatctgt cggggtgcca cagtggtgga tggaaaaaac gtttttccca
     7981 atatgaaact gtcgaaggaa aaatgcgacc aggtcaacgc cattgagcgt gataaggcgc
     8041 tggcatgggt ggagcgcaat ataaaagttc cactgaccga gccacagaaa gccggtatcg
     8101 cgtcattctg tccctataac attggccctg gtaagtgttt tccgtcgacg ttttataagc
     8161 ggcttaatgc cggtgatcgt aaaggcgcat gcgaggcgat tcgctggtgg ataaaggacg
     8221 gtggacgtga ttgccgcatt cgttcaaata attgttacgg tcaggttatt cgtcgtgacc
     8281 aggagagtgc attaacctgc tgggggatag aacagtgaat cagatattca cggtgatttt
     8341 gctcgtgttg gtaggatttg tcgtaggtaa tgtctggagc gacagaggat ggcaaaaaaa
     8401 atgggcggag cgtgatgctg ccgaattatc tcaagaggta aatgtccaat ttgctgctcg
     8461 aataattgaa caggggcgaa gtatatcccg tgatgaggct gttaaagatg cacaacagaa
     8521 agccgctgaa atttctgcca gggctgctga tctgtctgat agtgttaacc agctgcgtgc
     8581 cgaagcaaca aaatatgcca tacgccttga cgcagcgcag cataccgcaa atcttgccgc
     8641 tgccgtcaga ggcaaaacaa ccaaagccgc cgaaggaatg ctcaccaaca tgctcggaga
     8701 tattgcagca gaagctcagc tttatgctga aattgctgat aaacgctaca tc
//
LOCUS       DAJOWB010000164         8751 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJOWB010000164 DAJOWB010000000
VERSION     DAJOWB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.164"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(38..223)
                     /locus_tag="OHO85_004286"
     CDS             complement(38..223)
                     /locus_tag="OHO85_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCQ2673563.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(220..2859)
                     /locus_tag="OHO85_004287"
     CDS             complement(220..2859)
                     /locus_tag="OHO85_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCQ2673564.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQSPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3067..4056
                     /gene="ldhA"
                     /locus_tag="OHO85_004288"
     CDS             3067..4056
                     /gene="ldhA"
                     /locus_tag="OHO85_004288"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCQ2673565.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4167..4589
                     /gene="hslJ"
                     /locus_tag="OHO85_004289"
     CDS             4167..4589
                     /gene="hslJ"
                     /locus_tag="OHO85_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCQ2673566.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4586..4852)
                     /locus_tag="OHO85_004290"
     CDS             complement(4586..4852)
                     /locus_tag="OHO85_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCQ2673567.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5126..8650
                     /gene="nifJ"
                     /locus_tag="OHO85_004291"
     CDS             5126..8650
                     /gene="nifJ"
                     /locus_tag="OHO85_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCQ2673568.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLIRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGSWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSVALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2166 a         2293 c         2188 g         2104 t
ORIGIN      
        1 aaacgcacaa agaagtaatg ttttcttcat catcgcctca gaaaagatcg ctacgagttt
       61 caagcagctc ttcgacatct ttgtctgcct tgatgatgat ctcatgctca attttaacgt
      121 tcatattgat agtgatcggt tccttgggcg cagcgacttc aatacgcggc gtacaggcag
      181 ccagcataaa agatgacgtc aacgcagcca gtaaaatttt cattgttttt cctcacactc
      241 ttttccttgc ggacagtcat ttcccggcag acgtgcgttc tgctccaacc atgcctggag
      301 attatcgcca aagcgtaaac tgcgccacag cgtaaaaata ttctcttcat gatggtaatt
      361 aaggtttacc gtaccgcttt taccatcaac gcgactggta cctgtaatgt tggcctgcat
      421 ggttaataaa ccgagattat ctaaattaat atttgtcgat gaacggctaa tttccatata
      481 gcgcaaccag ttaattgctg aacccgcagt catattgtct ttcaccaccg catctgcggt
      541 gtctttatcc agccgcaagg tcattggccc gctatttgcc agccaaccat ctttcactat
      601 ccatttttcg ttgtttagcc ataacggcaa tgcaccacta aatgccccgg acatggcgaa
      661 ttgtttcgga ttgacggcgc taaccagttc gctggatgag agattattca gccgcaacag
      721 cgccgggtca tgttgcggca tacgtaattg tttcatcagt acgttaccgc ctaacacatc
      781 gacgctaaca tcagtcaaca gcaagggttc ttcttcagtc cacggataac gcccttgcaa
      841 atcagccgta atatttttcg ctgtcaccag attaatcact tcggcaattc gcaacgtaac
      901 ggggccgcga gtccccagat gccaggctcc atcggcaaaa cggaaaggca ggacaaaatc
      961 gacaccgtta acctgattat ctggcatcca ggcactaccg cctttcaaca cgccgtgtcc
     1021 tcccgcgcgg aatccttgtt caggcgcagc agaaaatgca acctgagcat acagttcacc
     1081 atcgcgtaag ttcatcttcc agtcgggtgg caccagcggc tggaatacag tcagtgattg
     1141 ttttggccac caggcgttac cgcgcagacg aataccgtcc cagcgaccat ttacccgaac
     1201 cgggccaatc tcaccagcat gtaaatcgcc tttaaagagg aaatacgtcg gatcgcgccc
     1261 atcgacgcta aattttaagg ttgatggcgg taacacactg ccgccagtga acagcgtttg
     1321 cccggcgtcc agtgacagcg cgccgctgaa ggagggatgc tgtgcgtcac gtacccagac
     1381 gacgggcttg tcgagaatta atcgcggctt ttctaccgtc atcgtaccgt attgtaactg
     1441 gtcgaaaccg gtggagaggt cggtcagcgt aatcgtgctg tcatgccatt caccttttcc
     1501 tgcgacatcc caggtggcat tcatcggtgt aaaactgcct tttccccagt agcgccactg
     1561 ccagcggccc gcgtcaggga gaaaatcatt cgccagccca tccatatgca gcacgaaatc
     1621 gcccagttca ttttcatgcg cctgcaagat ggcctgcaaa cgtccgtcaa caccacgttg
     1681 ggtgactttt acacccgcta aaggccagcg gatttcatcg atgtccagcg aatcgatgac
     1741 tcttcccttc gaacgtaata acgcgcctgg ctcaaaggcc aacgttgggt cagtcagact
     1801 tccgcttagc tgcgcaggta aacgggcata taaaatgagg tccgcctgtt tcgcttcacc
     1861 ggtcagttgc agaggcagct gactgttatc catgcttaat tttcctgggc caaaattaag
     1921 caccgcgttg cctttacccg cttgcccctg ggtcagcaca ctcagtcgtc cgctgaccag
     1981 agcgttctcg agccctgcct gccagttgtc gactttgaca cccagtcggc cactcaaagg
     2041 aaaacctgca tacggccagc tccagcgacc atcgcttacg gtcaattgtt gacgagtaat
     2101 ttgccacggc aaatcgagca acggatcgcc gttatcccgt gccagcacga tcaattgccc
     2161 gctattttcc tgccagtcca gctcggcatc caccagtgaa ggctcctgcg gcaagtttaa
     2221 cgtcgcagta gcatgaccac ttacaggaag tccatccggc acgagcggca tagtaaattc
     2281 ccccaccagt ttaaccggcg actgattttc aaacgcgacg acatccagtt cgctgactgt
     2341 aagttgttgc cctttcagct ggccttgaaa tttaactttt tcgccctgat aacgcagttg
     2401 ctggatatca gaggttaatg cgagagagag ttttccctgc cattcctgcc agggagaaaa
     2461 aatcagttta tcgatattga tccaggtgtt aggcagcatg gactgccact gcgcgagggt
     2521 ttttggagca gctggcgatt gctccgtctg cggcaatttc gccagacaag cagaatcaag
     2581 ttctaccgtg ccgacgttca ataaccagcg gctgggatgt gaaaggctgg cgttggtgat
     2641 atgcgcaagc tgacaatctc ccaccagata acggagatcg ggaatgatta aacctttacg
     2701 cgtaatgcgt gggctttcat ctaatgcaat acgtgtcccg agcggtagcc agatgcccgc
     2761 cagcgtggga acccacagcc cgagcgtcat cagcagcgtc aacggcacaa gaataatcag
     2821 taataacagc gcgagaacgg ctttatattt acccagcatg ggtagttaat atcctgattt
     2881 agcgaaaaat taagcattca atacgggtat tgtggcatgt ttaaccgttc agttgaaggt
     2941 tgcgcctaca ctaagcatag ttgttgatga atttttcaat atcgccatag ctttcaatta
     3001 aatttgaaat tttgtaaaat atttttagta gcttaaatgt gattcaacat cactggagaa
     3061 agtcttatga aactcgccgt ttatagcaca aaacagtacg acaagaagta cctgcaacag
     3121 gtgaacgagt cctttggctt tgagctggaa ttttttgact ttctgctgac ggaaaaaacc
     3181 gctaaaactg ccaatggctg cgaagcggta tgtattttcg taaacgatga cggcagccgc
     3241 ccggtgctgg aagagttgaa aaagcacggc gttaaatata tcgccctgcg ctgtgccggt
     3301 ttcaataacg tcgaccttga cgcggcaaaa gaactggggc tgaaagtagt ccgtgttcca
     3361 gcctatgatc cagaagccgt tgctgaacac gccatcggta tgatgatgac gctgaaccgc
     3421 cgtattcacc gcgcgtatca gcgtacccgt gacgctaact tctctctgga aggtctgacc
     3481 ggctttacta tgtatggcaa aacggcaggc gttatcggta ccggtaaaat cggtgtggcg
     3541 atgctgcgca ttctgaaagg ttttggtatg cgtctgctgg cgttcgatcc gtatccaagt
     3601 gcagcggcgc tggaactcgg tgtggagtat gttgatctgc caaccctgtt ctctgaatca
     3661 gacgttatct ctctgcactg cccgctgaca ccggaaaact accatctttt gaacgaagcc
     3721 gccttcgatc agatgaaaaa tggcgtgatg atcgtcaata ccagtcgcgg tgcattgatt
     3781 gattctcagg cggcaattga agcgttgaaa aatcagaaaa ttggttcgtt gggtatggac
     3841 gtgtatgaga acgaacgcga tctgttcttt gaagataaat ccaacgacgt gatccaggat
     3901 gacgtattcc gtcgcctgtc tgcctgccac aacgtgctgt ttaccgggca ccaggcattc
     3961 ctgacagcag aagctctgac cagtatttct cagactacgc tgcaaaactt aagcaatctg
     4021 gaaaaaggcg aaacctgccc gaacgaactg gtttaatctt gccgctcccc tgcattccag
     4081 gggagcttat tcagataatc cccaaagacc tttcatcttc tattcttaaa atagccctga
     4141 gtcagaaact gtaattgaga accacaatga agaaagtagc cgcgtttgtt gcgctaagcc
     4201 tgctgatggc gggatgtgta agtaatgaca aaattgctgt tacgccagaa cagctacagc
     4261 atcatcgctt tgtgctggaa agcgtaaacg gtaagcccgt gaccagcgat aaaaatccgc
     4321 cagaaatcag ctttggtgaa aaaatgatga tttccggcag catgtgtaac cgctttagcg
     4381 gtgaaggcaa actgtctaat ggtgaactga cagccaaagg gctggcaatg acccgtatga
     4441 tgtgcgctaa cccgcagctt aatgaactcg ataacaccat tagcgaaatg ctgaaagaag
     4501 gtgcacaagt ggatctgacc gcgaaccagt taacgctggc gaccgcgaaa cagacattaa
     4561 cttataagct ggcggattta atgaattaat agctgccaca gctcccggcg gcaagtgact
     4621 gttcgctaca gcgtttgccg ttgggtaatg cacacatccc aatcgccgta ccatccagtt
     4681 gacgggcaac agaaagcgaa ccgccgatca ttgcacaatt tgcttctcca ctactggaca
     4741 tcgacgcttt taaacctggc gctacgtgcg ccgcagtcgc ctgctgaaca ggttcactac
     4801 tacacgccga caacaataaa gcggcacacc ctacccaaaa cgctgctcgc atctcttctt
     4861 cctctgatct tcaagccaaa cgacaccgcc ataaataata ggcagcacag agggcggcgt
     4921 cgagagctgt cctgcgcgtt gccccgcatt tttacttttt tatggctatt tttttgccct
     4981 ctgtttgatc aaaacattca ttacgctgat gtgggggaca caaaagcgaa aatgcagaag
     5041 aaagccattt gctaaaattg aaggattact actgggcgcg cagcaatttc gtgcgcccct
     5101 catttgcgca atgtaagggt gtcatatgat tactattgac ggtaatggcg cggttgcttc
     5161 ggtggcgttt cgcaccagtg aagttatcgc catctaccct attaccccca gttccacgat
     5221 ggcagaacag gctgatgcct gggccggaaa cggcttgaag aacgtttggg gagacacacc
     5281 acgcgtggtt gaaatgcagt cggaagcggg tgctatcgct accgtgcatg gcgctttgca
     5341 gacgggtgcc ctttcaacat cgtttacgtc atcgcagggt ttgctgctga tgatcccgac
     5401 gctgtacaaa ctggcaggcg aactcacacc gtttgtcctg catgtagcgg cacgtaccgt
     5461 tgccacacat gcactctcta tttttggcga tcattccgac gtcatggcgg tgcgccagac
     5521 gggttgcgcg atgttgtgtg cggcaaacgt ccaggaagcg caagactttg ctctcatttc
     5581 gcacatcgcg acgctgaaaa gccgcgtgcc atttattcat ttctttgatg gtttccgcac
     5641 gtcccacgaa atcaacaaaa ttgtcccgct ggccgatgac acgattcttg atctcatgcc
     5701 gcaggccgaa attgatgctc atcgcgcccg ggcactcaac ccggaacatc cggtgatccg
     5761 cggtacgtcc gccaatcctg acacttattt ccagtctcgc gaagccacca acccatggta
     5821 cgacgcggtc tatgaccatg ttgaacaggc gatgaatgat ttctctgccg cgacaggtcg
     5881 tcagtatcag ccgtttgaat attacgggca tccgcaagcg gaacgggtga ttatcctgat
     5941 gggctctgcc attggcacct gtgaagaagt ggttgatgaa ttgctaatcc gtggcgaaaa
     6001 agtcggcgtg ctgaaagttc gcctgtaccg ccccttctcc gccaaacact tgctgcaagc
     6061 tctgccggga tccgtacgca gcgtggcggt actggacaga accaaagaac ccggtgccca
     6121 ggcagaaccg ctctatctgg atgtgatgac cgcactggca gaagccttta ataatggtga
     6181 gcgcgaaacc ctgccccgtg tcattggtgg gcgctatggt ctttcatcca aagaatttgg
     6241 cccggactgt gtactggcgg tatttgccga gctcaacgcg gctaaaccga aagcgcgctt
     6301 tacggttggt atttacgatg atgtgactaa tctgtcactg ccgttgccgg aaaacaccct
     6361 gccaaactcg gcgaaactgg aagccttgtt ttatggcctt ggtagtgatg gcagcgtttc
     6421 cgcgaccaaa aacaatatca agattatcgg taattccacg ccgtggtacg cacagggcta
     6481 ttttgtttac gactccaaaa aagcgggcgg cctgacggtt tctcaccttc gcgtgagcga
     6541 acagccgatt cgttccgctt atctcatttc ccaggctgat tttgttggct gccaccagtt
     6601 gcagtttatc gataaatatc agatggctga gcgtttaaaa cctggcggca ttttcctgct
     6661 caacacaccg tacagcgcag atgaagtgtg gtcgcgcttg ccgcaagaag ttcaggccgt
     6721 gttaaaccag aaaaaagcgc gcttctatgt gattaacgcg gcgaaaatcg cccgcgaatg
     6781 tggcctggcg gcccgtatta ataccgtcat gcagatggct tttttccatc tgacgcaaat
     6841 tctgcctggc gatagcgccc tcgcagaatt gcagggtgcg attgccaaaa gttacagtag
     6901 caaaggccag gatctggtgg aacgcaattg gcaggctctg gcgctggcgc gtgaatccgt
     6961 agaagaagtt ccgttgcaac cggtaaatcc gcacagcgcc aatcgaccgc cagtggtttc
     7021 cgatgccgct cctgatttcg tgaaaaccgt aaccgctgcg atgctcgccg ggcttggtga
     7081 cgccctcccc gtttcggcgc tgccgccaga cggctcctgg ccgatgggca ctacgcgctg
     7141 ggaaaaacgt aatattgccg aagagatccc catctggaaa gaggaactct gtacccaatg
     7201 taaccactgc gttgccgctt gcccacactc agctattcgc gcaaaagtgg tgccgcctga
     7261 agcgatggaa aacgcccctg ccagcctgca ttcgctggat gtgaaatcgc gtgatatgcg
     7321 cgggcagaaa tatgtcttgc aggtggctcc ggaagattgc accggttgta acctgtgcgt
     7381 cgaagtttgc ccggcgaaag accgtcagaa tccagagatt aaagccatca atatgatgtc
     7441 gcgcctggaa catgtcgaag aagagaaaat caattacgat ttcttcctca acctgccaga
     7501 aatcgaccgt agcaaactgg aacgtattga tattcgtaca tcgcagctga ttacaccgct
     7561 gtttgaatat tcaggtgctt gctccggttg tggcgagacg ccgtatatta aattactgac
     7621 tcagctctat ggcgaccgga tgttgatcgc taacgccacc ggctgttctt caatttatgg
     7681 cggtaacctg ccctctacac cgtataccac cgatgccaac ggtcgtgggc cggcatgggc
     7741 gaactctcta tttgaagata atgccgaatt tggccttggt ttccgcctga cggtcgatca
     7801 acaccgtgtc cgcgtgctgc gtctgctgga tcaatttgcc gataaaatcc cggcggaatt
     7861 actgacggcg ttgaaatcag acgctacgcc agaggttcgt cgagaacagg tcgcagcttt
     7921 acgccagcaa ctcaacgatg ttgccgaagc acatgaactg ctacgtgatg cagatgcact
     7981 ggtggaaaaa tcaatctggc tgattggtgg tgatggctgg gcttacgata tcggctttgg
     8041 cggtctggat catgtattga gtttgacgga aaacgtcaac attctggtgc tggatacgca
     8101 atgctattcc aacaccggtg gtcaggcatc gaaagcgaca ccgctgggtg cagtaactaa
     8161 atttggcgag cacggcaaac gtaaagcgcg taaagatctt ggcgtcagta tgatgatgta
     8221 cggtcatgtt tatgtggcgc agatttctct cggcgcgcag ctgaaccaga cggtgaaagc
     8281 gattcaggaa gcggaagcgt atccggggcc atcgctgatc attgcttata gcccgtgtga
     8341 agagcatggt tacgatctgg cactcagcca cgaccagatg cgccaactca cagctaccgg
     8401 cttctggccg ctatatcgct tcgatccgcg tcgtgccgat gaaggcaaac tgccgctggc
     8461 gctggattca cgcccgccgt cagtagcact ggaagaaacg ttacttcacg agcaacgttt
     8521 ccgtcggctg aattcgcagc agccagaagt ggcagaacag ttatggaaag atgctgcagc
     8581 tgatttgcaa aaacgctatg acttcctggc acaaatggcc ggaaaagcgg aaaaaagcaa
     8641 caccgattaa atgctctgga taaggattat ccaattctaa aaaaaaagcc cggacgactg
     8701 ttcgggcttg tctttttata tgttggaaaa tcagtggcaa tgcaatggcc c
//
LOCUS       DAJOWB010000165         8660 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJOWB010000165 DAJOWB010000000
VERSION     DAJOWB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.165"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..469
                     /locus_tag="OHO85_004292"
     CDS             <1..469
                     /locus_tag="OHO85_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCQ2673569.1"
                     /translation="ENLTSALSQRLIADQLRLTTAESCTGGKLASALCAAEDTPKFYG
                     TGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAIERADADVSIAITGYGGP
                     EGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAVRFALVQLLQLLL"
     gene            complement(514..2559)
                     /gene="dcp"
                     /locus_tag="OHO85_004293"
     CDS             complement(514..2559)
                     /gene="dcp"
                     /locus_tag="OHO85_004293"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCQ2673570.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLSGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAVAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            2696..3442
                     /gene="ydfG"
                     /locus_tag="OHO85_004294"
     CDS             2696..3442
                     /gene="ydfG"
                     /locus_tag="OHO85_004294"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCQ2673571.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3531..4217
                     /gene="ydfH"
                     /locus_tag="OHO85_004295"
     CDS             3531..4217
                     /gene="ydfH"
                     /locus_tag="OHO85_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCQ2673572.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            4395..4598
                     /gene="ydfZ"
                     /locus_tag="OHO85_004296"
     CDS             4395..4598
                     /gene="ydfZ"
                     /locus_tag="OHO85_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCQ2673573.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4634..6094)
                     /locus_tag="OHO85_004297"
     CDS             complement(4634..6094)
                     /locus_tag="OHO85_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCQ2673574.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPTVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(6183..7466)
                     /gene="ydfJ"
                     /locus_tag="OHO85_004298"
                     /pseudo
     CDS             complement(6183..7466)
                     /gene="ydfJ"
                     /locus_tag="OHO85_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            8070..8183
                     /locus_tag="OHO85_004299"
     CDS             8070..8183
                     /locus_tag="OHO85_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673575.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
     gene            8252..8485
                     /gene="ydfK"
                     /locus_tag="OHO85_004300"
     CDS             8252..8485
                     /gene="ydfK"
                     /locus_tag="OHO85_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HCQ2673576.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNQNLNGKM"
BASE COUNT         2347 a         2182 c         1927 g         2204 t
ORIGIN      
        1 tgaaaacctg acatccgcgt tgagtcaaag acttatcgcg gatcaattac gcttaactac
       61 cgccgaatca tgcaccggcg gtaagttggc tagcgccctg tgtgcagctg aagatacacc
      121 caaattttac ggtacaggct ttgttacttt caccgatcag gcaaagatga aaatcctcag
      181 cgtaagccag caatctcttg aacgatattc tgcggtgagt gagaaagtgg cagcagaaat
      241 ggcaacaggt gccatagagc gtgcggatgc tgatgtcagt attgccatta ccggctacgg
      301 cggaccggag ggcggtgaag atggtacgcc agcgggtacc gtctggttcg cgtggcatat
      361 taaaggccag acctacactg cggttatgca ttttgctggc gactgcgaaa cggtattagc
      421 tttagcggtg aggtttgccc tcgtccagct gctgcaatta ctgctataac caggctggcc
      481 tggcgatatc tccaggccag ccattggtgg tgtttatatg ttcaagccac gatgttgcag
      541 catcggcata atctgaggag ccttaccgcg ccattgtcga tacagccgtt ccagatcttc
      601 gctgttacct ctggaaagga tcgcctcgcg aaaacgctgc ccattttcac gcgttaatcc
      661 gccctgctca acaaaccact ggtaaccatc atcggccaac atttgcgtcc acagataagc
      721 gtaataacct gcagcatatc cgccaccaaa aatatgggcg aaataactgc tgcgatagcg
      781 tggcggtata gcaggaagat ccatattttc cgccaccagc gcccgcaatt caaaatcatc
      841 gacatcctgc attgcttcgt tttcttccag gcaatgccag cgcatatcga gaagtgcggc
      901 gctaagcagt tcgctcatct catacccttt gttgaacagg ctggcattac gcattttctg
      961 ttgcagttcg tcaggcattg ctgccccgct ctgataatgc cgggcgtagc gagcgaatac
     1021 ctgcggatgc gttgcccagt gttcgttgat ttgcgacgga aattcgacaa aatcacgcgg
     1081 cgtgttggtg ccggaaagcg tggcataacg ctggcgggca aaaaggccgt gcaacgtatg
     1141 accaaattca tggaataagg ttatgacatc atcccagagt aacaacgcag gctcaccggc
     1201 agcgggtttc tgataattgc aaacgttata aattaccgga tgcgtttcat taagcgttga
     1261 ttgctcaaca aaattgccca tccatgcacc gccgcttttt gaatcacggg cgaagaaatc
     1321 accgtaaaat aacgccagcc ccacgccatt atgatcaaaa atttcccaca cccgaacgtc
     1381 aggatggtag acaggaatat caaaacgttc gacaaactta ataccgaaga gctgattcgc
     1441 ggtccagaat acaccttcat tcaacaccgt gtttaattca aaatatggct tgagctgcgc
     1501 ctcatcaaga tcaaatttct cccgccgtac ctgttccgca taaaatgccc agtcccacgg
     1561 ctgcgcgcta aacccgccct gctgcttatc gataaccgcc tgtatggagg ctaattcatc
     1621 gctcgcacgt tgacgcgccg ctggaacaat ttcccgcata aagttgagtg ctgcttctgg
     1681 cgtttttgcc atctgatcgg cgattttcca tgcggcataa tgaggaaaac caagcagttt
     1741 cgcctgctgt gcgcgaatct ctaccagacg ttgaatgata gcgcgggtat cattgccatc
     1801 atttttttcc gctcgcgtcc agcccgcaat aaacagtttt tcacgcgtcg cacgatcgcg
     1861 catttcggca agcgccggtt gttgggtggt attcagcagc ggaatcagcc atttgttatc
     1921 cagacctttc tcgcgagccg ccactgccgc cagcgcaatc tcttgctcac tcattcctgc
     1981 cagctgcgcg atatcgttca caaccagacc gccggattta tttgctgcaa gtaaccgctg
     2041 gttaaactgg ctggtcaggg tcgcagcttc tgtattcagt acttttaatt ttgctttatc
     2101 agcttgcgca agtttggctc cggcaaggac aaaacgttga tgaataacct ccaccaggcg
     2161 gatggattca ctatcaagcc ccagggattc acggcgctgc cagacagcat ctacccgcgc
     2221 gaataattca ccgctcagat agatatcatt agccagttcc gccagttcag cggaaaactg
     2281 ttcatcaaga cgctgtaatt catcattggt atgcgccgca gtcatcgcaa aaaagacgct
     2341 ggtaacgcgg gtaagtaatt ctccgctttg ttccagtgcc agaatagtat tgttgaaatc
     2401 aggcgtttgc gggttaagcg cgatggcagc aatttctgcc cgcttttgct gcattccctc
     2461 atcgaatgcc gggcgatagt gatgattggc aatttgatca aaatgaggag ccagatacgg
     2521 cagtgtgctt tgcacaagga aaggattcat tgttgtcatt ttcttctcct gaacgcgagg
     2581 tgttccatag cgtaggctta ctgataacgg agtgcaatct tgcaaaccag tattacccgc
     2641 tcttaagcat cacgtgctat gttattgaca cacaaaagcg ttgaggaaca gtgagatgat
     2701 cgttttagta actggagcaa cggcaggttt tggtgaatgc attactcgtc gttttattca
     2761 acaagggcat aaagttatcg ccactggccg tcgccaggag cggttgcagg agttaaaaga
     2821 cgaactggga gataatctgt atatcgccca actggacgtt cgcaaccgcg ccgctattga
     2881 agagatgctg gcatcgcttc ctgccgagtg gtgcaatatt gatatcctgg taaataatgc
     2941 cggcctggcg ttgggcatgg agcctgcgca taaagccagc gttgaagact gggaaacgat
     3001 gattgatacc aacaacaaag gcctggtata tatgacgcgc gccgtcttac cgggtatggt
     3061 tgaacgtaat catggtcata ttattaacat tggctcaacg gcaggtagct ggccgtatgc
     3121 cggtggtaac gtttacggtg cgacgaaagc gtttgttcgt cagtttagcc tgaatctgcg
     3181 tacggatctg catggtacgg cggtgcgcgt caccgacatc gaaccgggtc tggtgggtgg
     3241 caccgagttt tccaatgtcc gctttaaagg cgatgacggt aaagcggaaa aaacctatca
     3301 aaataccgtt gcattgacgc cagaagatgt cagcgaagcc gtctggtggg tgtcaacgct
     3361 gcctgctcac gtcaatatca ataccctgga aatgatgccg gttacccaaa gctatgccgg
     3421 actgaatgtc caccgtcagt aatttttata cccggcgtaa ctgccgggtt attgcttgtc
     3481 acaaaaaagt ggtagactca tgcagttaac tcactcacaa gcaagaacga atgaccgtcg
     3541 aaacgcaact taatcccaca cagcctgtca atcagcagat ttatcgtatt cttcgtcgcg
     3601 acattgtcca ttgcctgatt gcaccaggca caccgttgtc ggaaaaagaa gtttctgttc
     3661 gtttcaatgt gtcacgccag ccggttcgtg aagcctttat taaactggcg gaaaacggcc
     3721 tgattcaaat tcgtccgcag cgtggcagct acgtcaacaa aatttccatg gcccaggtgc
     3781 gaaacggcag ttttatccgt caggccattg agtgcgcggt ggcgcgtcgg gcggcgagca
     3841 tgattaccga aagccagtgc taccaactgg aacaaaatct tcaccagcaa cgcattgcca
     3901 ttgagcgcaa gcaactggat gatttttttg aacttgatga caacttccat caactcctga
     3961 cgcagattgc cgactgtcaa ctggcgtggg ataccattga gaacctgaaa gcgaccgttg
     4021 atcgcgtgcg ctatatgagt ttcgaccatg tttctccacc agaaatgctg ttacgccagc
     4081 atcttgatat tttctctgcc ctgcaaaaac gtgatggcga tgcggtagaa cgtgcaatga
     4141 cgcaacattt gcaggaaatc agcgaatccg tgcgccagat ccgccaggaa aacagcgact
     4201 ggtttagcga agagtaattc atttactctc atcccatccg ggatgagagg cttttccccc
     4261 cgccttacgg ctcatgcatg catcaaaaaa gatgtgagct tgatcaaaaa caaaaaatat
     4321 ttcactcgac aggagtattt atattgcgcc cgttacgtgg gcttcgactg taaatcagaa
     4381 aggagaaaac acctatgacg acctacgatc gtaaccgtaa cgcaatcacc actggcagcc
     4441 gtgttatggt tagcggcacc ggtcacactg gcaagatcct gtcgattgat actgaaggtc
     4501 tgaccgctga gcaaatccgc cgcggaaaaa ccgtagttgt tgaaggttgt gaagagaaac
     4561 tggcacctct ggacctgatt cgtctcggca tgaactaagt gtgtgaatgc cgccgatggc
     4621 ggcattgctt tttttacttc acggaatatt tcgccacggt cgctttcgcg ccatgcgcta
     4681 ataaagacaa gtacgcttcc gtcacttttg cagtaaacaa gctattgtct ggcaaatcat
     4741 caccaaagat tgccttaatc gccagcaatg actggacgcg cgctttccct tcggcactac
     4801 tttgtacagc cttctgaata acaggtaaca gtgggtcact gatttctatc ggatttccct
     4861 gttcatcaac accaccgaca taacgcatcc aacccgcgac gcccagcgcc agcagatcga
     4921 acttgctgtc atgcgccaga tgccagcgaa cagaatccaa catccgctgt ggcaatttct
     4981 ggctaccatc catcgcaatc tgccaggttc gatgacgtaa cgccgggttg ctatagcgtg
     5041 caattaatcg gttagcgtaa tcttgcaaat caacgccctg cactttcagc gtcggcgctt
     5101 gttcctgcaa catcaagcca tacgccgcat aacgataatg ttcatcttcc atgcagtcat
     5161 taatgtgctg atatccagca agataaccca aatacgccag gaatgaatga ctgccgttga
     5221 gcatgcgcaa cttcatctct tcataaggca gcacatcact aaccagttcg gctcccgctt
     5281 tttcccattc tggacgtccg gcaacaaagt tatcttctat tacccactgg cggaaaggtt
     5341 cacaggcaac gcccgcagga tcgcccacac cggtaagttg ttcgattttc gccagcgtat
     5401 cctctgtcac tgtgggcaca atacggtcca ccattgttga tgggaaagtc acgttatctt
     5461 cgatccattg tgccagtttt acatcaacag ctttcgcgta tgaagtgaca acgtcacgca
     5521 taacatgacc gttttctggc atgttgtcac atgacatgac ggtaaatgcg ggaagtcctg
     5581 ccgctttacg gcgggccagc gcctcaacaa ttacccctgt tgctgttttc ggctggtggg
     5641 gattttgcac gtcggcagcg accatcgggt gatcgagcat taactgtccg gtcgccggag
     5701 agtggaaata ccctttttcg gtgattgtca gagagacaat cgcgatttgc ggttcacaca
     5761 tcgcagccaa cacggtttct aagccatcca tctgtacgtg caaggctttt ttaacgacgc
     5821 caacgacgcg agccgtccac gcatcggccg acatttccgc aacggtataa agattatctt
     5881 gctgttgtaa atcggcaatt tgctgttcgc cgccgattaa gttgacctca taatatcccc
     5941 agtcactgaa atgttctgta gcaagaatat cggcatacac gccctgatgc gcacggtgaa
     6001 atgcaccaaa gcctaaatga ataattcttg gggccaggtt attacgatca taaacaggga
     6061 gtgtcgcttt tgctgataac aaattatttc ccataacaat tccttaaatt taaatatggc
     6121 aagctatatg ttttgttata tgaataaaaa tcccctctcc ggtaagagaa gggattgagg
     6181 gtttacagac ttctggaagg ttgcgcagct cttacaacac gcggttgatc ttccgcagcg
     6241 tcttccagcg cacttaaatc acggtctttc acctctggca ttttcagcgc agagattata
     6301 ccaattactg aatatgccat gatcataatg gcgatcggat accaggattc cgtcatggtg
     6361 cagaaaatac ccgccaggat aggaccaaaa ccggaagcga taagaccacc aatttcttta
     6421 gaaatagcca tccgggtaaa gcggttttta cagccgaaca tttctgccat ggtaatgttt
     6481 tccagagcaa ataatcccag caccgcacag ttatgaatca caatcagtgc aaccataatg
     6541 gtgctcgggg catagctttt atctacaatg atagaaagca ttggccatgc cagcacaatc
     6601 gcggaggtat tcataataat atacgggatc cggcgaccaa ttttatcgga taaccaacca
     6661 aggaacggaa tggtcataaa gccgagaatc gaactaatca tcaatgcatc tgttggaatt
     6721 gctttgttaa acaataacgt ctgcactaaa tagcctgcaa ggaaagtctg aattaacccg
     6781 gagttacccg cctgaccaaa acgcagccct gttgccagcc agaaggattt gctctggaac
     6841 atgctaccag caggtgcagg ttttgctgtg ggttggttgc tgtcattaac cttctcaaag
     6901 accgggcttt ctttcagatt catacgtaac cagatagcaa agaccatcac gacaacgctc
     6961 gccaggaacg gtatacgcca tccccacgcc agcagttcct ctttactgag aatgaagaac
     7021 ataaaggccc agattgccgt tgcgctcaag gttccgcagt tagttcccat agctacaaat
     7081 gaggagataa ttccgcgctt accttttggt gcatattccg ccagcatcgt accggcaccg
     7141 gaaatttccg cacctgcacc caacccctga ataatacgca gcgtcaccag caagatgggg
     7201 gcaaaaacac caatctgtgc ataggtcggt aacacaccaa ttaaggtggt acagatcccc
     7261 atcatggtga tggtaataaa gagcactttt ttacgcccta ttctgtcgcc cattttgccg
     7321 aaaataaatg ctccgacaat acgcgccaca taacctgcac cgtaggttcc cattgccaaa
     7381 attaacgcca ttgccgttga tgattcagga aaaaatattt catgaaacac caacgctgcg
     7441 ccgagcgaat ataactggaa atccataatt cacaggtgtt ttttcccatc ctgtggtttc
     7501 cttggcgttt tctaggtttt ttcagatagt tgcatttttt aaaaagcatc ctaagttcga
     7561 tctcagtgtc tatctggggc ctatttctgt cccatatatg ccccaaaaaa actccccaac
     7621 agataagtag ttttttcatg gatttatgcg taaaatcaag aacggctgga aatcattcaa
     7681 tactcacact atcgaaaaat ttaccagcca accgcagcac gttcttgcat aaggcgtgtc
     7741 tgcggttttt caactattca gatacatcac tcccatcaca ttcattcctc cgcatcaaag
     7801 gcatataggc tatatcacct tgatattttt cttcttcaga taaaaactgt tatctatgta
     7861 tacttttaaa cccaatccgt gtagagtctc tgcataagat agtttgcagt tgccgcttca
     7921 gcttgcgcca taaaccgcct gatttttgct gccacctgtt agcattcctg tatacctgaa
     7981 acgacaatgt ttatctacga actttaagaa cacccaagat aaaaattgtc aactatatca
     8041 tatataacac attactaatt cgaggctata tgaacagcat actgataatc acttctctcc
     8101 ttatcatatt cagcattttt agtcatgccc taataaaatt agggattggc atatccaata
     8161 acccagacaa aaccgatgta taagccaaca tatcctgaat cagacataca atatcgcaat
     8221 gaaaatcaat aatattttaa ggaatatctt catgaaatca aaagacaccc taaagtggtt
     8281 ccctgcgcag cttcctgaag taagaattat cctaggggat gctgtagtgg aagtagcaaa
     8341 acagggaaga cctatcaata ccagaacatt gcttgattac attgaaggaa acataaagaa
     8401 aaaatcatgg ctggataaca aagaattatt acaaacagcg atatcagttc ttaaagacaa
     8461 ccaaaattta aatggtaaaa tgtaatataa taaacttact tttttatcat ttttccactt
     8521 taacaacatt ttgctccact tttccacgac caaacaactt gaaatctggt taaaataaca
     8581 cgcaacacta ttcttcttcc ttgagtccgc ccggaactcg aaaaacaaac cgagttaaag
     8641 ccatttttca caaaatcgat
//
LOCUS       DAJOWB010000166         8651 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJOWB010000166 DAJOWB010000000
VERSION     DAJOWB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.166"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(323..1213)
                     /locus_tag="OHO85_004301"
     CDS             complement(323..1213)
                     /locus_tag="OHO85_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2673577.1"
                     /translation="MFISDETIRIIHLVARHQNITTAAEQLNKVPSTISYTLKKAEET
                     LGVELFIRKGRYIELSPAGSYFIEHSKNILRDLDALKRNTVLIHDGIERELTIAVNNI
                     IPGDFLVSFIQDFERQFTSTTLTVDIEVYNGCWDALYSKRADLVYGAPHAVPSSEGII
                     SEPVGQIEWDFVVSPLHPLAVKRNPLENSELRHYPAVCIRDTSVNFPPMQAWLLEGQK
                     PIFVPDFAMAISLIEQNVGIGYIPHHLALPLLNRGKLLKKPMREHKHATKLFLAARSD
                     GIGKACLWCMSYLRNKQFMA"
     gene            complement(1291..2514)
                     /locus_tag="OHO85_004302"
     CDS             complement(1291..2514)
                     /locus_tag="OHO85_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HCQ2673578.1"
                     /translation="MMRKEDNMNTASGLRITMILLGIAVTPVLLSSSSLGNQLSSGSL
                     ISVVLLGGVILTLLSAITISVGEKARLPTYGIVKYSFGEKGAIAINILMAISLFGWIA
                     VTANMFGHSVHDLLAQNGIEAPLALLVAAGCVIFVASTAFGFSILGKVAQVAVPVIAL
                     VLCYILYVATHTEVSAPAAIAEMSTGVAVSTVVGTIIVLVATLPDFGSFVHNRKHALI
                     AAGMTFLVAYPLLYWAGATPSAISGQGSLLNAMAVFGAVMPAALLLIFACVTGNAGNM
                     FQGTLVVSTLLSRFPKWQITVALGILSAIVGSMDIMAWFIPFLLFLGIATPPVAGIYI
                     ADFFLYRRNGYQESVLTQESQVKVLTFAAWITGTAVGFMTVKGLFTLTTIPSVDSILV
                     ACIAYAILSRASQHR"
     gene            complement(2537..2926)
                     /locus_tag="OHO85_004303"
     CDS             complement(2537..2926)
                     /locus_tag="OHO85_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HCQ2673579.1"
                     /translation="MTTTLLAVNGTLMRGLELNPNMQKAGGIFVREDRTDAHYRLWSI
                     NDRHPGMIRVNEGGAHVDVEIWQLPLASFAELLMNEPEGLTIGKIKLADGSEVLGVLA
                     ESWLTEGQKEITALGGWRKYTGNFHTM"
     gene            complement(2943..3899)
                     /gene="arcC"
                     /locus_tag="OHO85_004304"
     CDS             complement(2943..3899)
                     /gene="arcC"
                     /locus_tag="OHO85_004304"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCQ2673580.1"
                     /translation="MVKPLAVVAVGGNALIQDDKRNSIPDQYVAVMESVQHIVDMVEA
                     GWELVLTHGNGPQVGFILRRSELASNEVSPVPLDYAVGDTQGAIGYMFQKALHNELAR
                     RGINKPVIALVTQTRVSPYDDAFASPSKPIGAFLDEETAQQRQQQLGWTLMEDAGRGW
                     RRTVPSPAPLEIIEHHTIAHLVRQGYLVIACGGGGIPVVRDGQQLKGVEAVIDKDLAS
                     ALLASQLGADLLVIPTGVEKVAIHFGTPQQQWLDAISVYEAQTLLQEGQFGVGSMQPK
                     VEAIIDFINASQQQGKQASGLITSPQTIKAALAHQSGTWITL"
     gene            complement(3892..5316)
                     /locus_tag="OHO85_004305"
     CDS             complement(3892..5316)
                     /locus_tag="OHO85_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCQ2673581.1"
                     /translation="MNTLFNQPLKVVNAGLHSFADNIQHAGGHAISLNWQPPAQGDID
                     TGLVLASLLRHPLVESANQIAMTRYLEAQPVLVDVMLAKEAIPEMAEQKRILHSGPPI
                     AWEDMCGPVKGAIIGAMLYEGWATSQKDAENQINAGEIDLAPCHHYHAVGPMAGIISP
                     SMPLWVVENKTNGHRTFSNFNEGLGKVLRFGANNDEVLNRLAWMRDELAPAMKAAIAQ
                     HGELELKPLMAQALHMGDEVHNRNAAATGLLIKRLLPALLACSLPQEHIQRVVAFITG
                     NDHFFLNLSMAACKAMMDAAANVPFSSMVTVMARNGVNFGIRLSGTGDRWFQAPANAV
                     EGLFFPGFGVDDAASDLGDSAITETAGVGGFAMASSPAIVKFVGGTPADATNNSRRMQ
                     AITLGGNPAFTLPALNFAPTAAGIDACKVTDRGILPVINTGIAHKQAGVGQIGAGITT
                     APMACFVAAVRELAEIVAKENHHG"
     gene            complement(5313..6872)
                     /gene="fdrA"
                     /locus_tag="OHO85_004306"
     CDS             complement(5313..6872)
                     /gene="fdrA"
                     /locus_tag="OHO85_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HCQ2673582.1"
                     /translation="MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNL
                     EQLRDAGLGNEINASPNDLVIAVMGEEDICNEALLLAQQRLTSKPDDETDCGIKSPEK
                     VSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIGQEKYIKTLA
                     RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGIS
                     QALGTGGHDLSEEIGGISMLFALDALAQDDETHVIVLISKPPSPIVARTILERAEACG
                     KPVVVNFLGANPHDLARPNITAATTLASAADIAVALLNDQPLPAVENEVSCDDLTMLQ
                     NACQSLPVHRQAIRGVFAGGTFCYEAQLICQQKGFHAASNTPVAGNRALANIWQSEDH
                     TLIDMGDDDFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGTSATPVSEL
                     LDQLSHIDMNNAPLLIAHVCGTEADPQIRSQQISSLQNAGVIIANSNAQAALWASTVA
                     QTQLQKKELNA"
     gene            complement(6967..7827)
                     /locus_tag="OHO85_004307"
     CDS             complement(6967..7827)
                     /locus_tag="OHO85_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCQ2673583.1"
                     /translation="MVQVTLCASSLGYLFPQNETQDLRLHSAFKHAVNLYSDAGTFIT
                     LLCAQTYLNLPDAARVWLPECWDWRREIAHSDPIQLTPGLLRTPRFCVALDNATLWQS
                     PFVGGMLTLEALPLVFQHYPTIASQRLLFCLEHNVQSTLHLPDSLTHQGLDIMEHPDA
                     LERQVPQLIGFGKGLTPDGDDYLLGYLAALWLWQLPAPLANHQYRLQKAIEQHAHNTT
                     DISRHYLQRALQGHFSEPICRLLAQLVGSASAMTIASCTEQIMQFGATSGVNCLAGML
                     HGFRTLNTMN"
     gene            complement(7833..8501)
                     /locus_tag="OHO85_004308"
     CDS             complement(7833..8501)
                     /locus_tag="OHO85_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCQ2673584.1"
                     /translation="MTISIFQAQPFELPFDPCTTALIMIDMQRDFVEAGGFGEALGND
                     VSLVRTAIAPCKEVLAAARQKGIMVIHTREGHREDLSDCPSAKLTRGGKTFIGEPGPM
                     GRILVRGEAGHDIIPELYPVTGEPVIDKPGKGAFYQTDLHLILQKRGIKTLIVCGVTT
                     EVCVTTTVREANDRGYECIIPEDCVGSYFPEFQKYALEMIKAQGAIFGWVTDSKAIIA
                     GLEG"
BASE COUNT         2163 a         2167 c         2111 g         2210 t
ORIGIN      
        1 cattttatca agaagaatat caaattttct ccgatacttc tacatactcc atcattcatg
       61 ctccttttat ccgtgcaccg taaaaccccg tccttcagcg gggatataag gcgctgtttt
      121 ccacctaacc aggtgtctgc tgctattcaa tgtattggcg gatgacggat gccgcccgtt
      181 gaagcaggaa ccctcgtcct tagggcgtgg aggatgtcaa aatgaaaact tgtggtatga
      241 atcaatccat tcatcatcct ttcggatcat tgcattcagt ataaccctgg aagagcctgg
      301 caatatcact gtattgatgg tattacgcca tgaattgctt atttcttaaa tacgacatgc
      361 accacagaca ggcttttcct ataccatcgg aacgagcggc aagaaataac ttggtggcgt
      421 gtttatgttc gcgcattggc tttttaagca atttacctct gttcaataac ggcaatgcca
      481 gatgatgtgg aatataacca ataccgacgt tttgttctat taagctgatt gccatggcga
      541 agtcaggaac aaaaataggt ttttgtcctt ccaacaacca ggcttgcatt ggggggaagt
      601 taacggaggt atcccggata caaaccgcag gataatgacg caattcactg ttttctaatg
      661 gattccgctt cactgccagt ggatgaagtg ggctgacgac aaaatcccat tctatttgtc
      721 cgaccggttc gctaatgatg ccttcactgc ttggtactgc atgaggagca ccgtagacca
      781 gatcagcacg tttactgtac agcgcatccc agcagccgtt gtatacttca atatctaccg
      841 tcagtgttgt tgatgtgaat tgccgttcaa aatcttgaat aaatgacacc agaaaatcac
      901 caggtataat attgttcacc gcaattgtca gttcacgctc aataccatcg tgaatcagca
      961 ctgtgttgcg ctttaatgca tccagatcgc ggagaatatt tttgctatgt tcgatgaagt
     1021 aactccccgc cggagataat tcaatatatc gtcccttacg aatgaacagt tcaacgccca
     1081 gggtttcttc cgctttcttc agggtgtaac tgatagtaga agggacttta tttaactgtt
     1141 cggctgcggt ggtgatgttc tggtgtctgg cgaccagatg aataattcga atcgtttcgt
     1201 ctgagatgaa cataaggctc cctggtcagt ggtaaagtgg ctgaatctct ccagattcag
     1261 ccgtaatcat tgcctgagta tacccaggaa ttagcggtgt tgacttgccc gactgagaat
     1321 cgcatacgcg atacatgcca ccagaatgga gtcaactgaa gggatcgtcg tcagggtgaa
     1381 taagcctttt acggtcataa agccaaccgc tgtgcctgtg atccatgcag cgaatgtcag
     1441 tactttaacc tgtgactcct gagttaacac cgactcttga tagccattac ggcgataaag
     1501 gaaaaagtca gcgatataaa ttccggcaac tgggggagtc gcgataccca ggaacagcag
     1561 aaacggaata aaccacgcca taatatccat actgcctacg atggcggaaa ggatacccag
     1621 cgcaacggta atctgccatt tgggaaagcg gctaagcagt gtggaaacta ccaacgtccc
     1681 ctggaacata ttgcccgcat taccggtgac gcaggcgaat atcagcaaca gtgctgcagg
     1741 cataaccgca ccgaatactg ccatagcatt cagtaaagag ccctggccgc taatggcact
     1801 cggcgttgca cccgcccagt atagcagagg gtaggcaacc agaaacgtca tacctgcggc
     1861 aatcagagca tgttttcggt tatgtacaaa actaccgaag tcgggaagtg tagcaaccag
     1921 cacaataata gtgccaacaa cggtggaaac ggcgacacct gtactcatct ccgctatcgc
     1981 tgctggcgct gatacttcgg tatgggttgc cacatagagt atataacaca gcaccagtgc
     2041 gataaccggc acggcgacct gggcaacttt gcccagaatg gaaaaaccaa aggccgtaga
     2101 ggcgacaaaa atgacacaac cagctgccac taacagtgcc agtggcgctt caatcccatt
     2161 ctgcgccagt aagtcatgca ccgaatgacc aaacatatta gcggtaacgg caatccagcc
     2221 gaacagactt atagccatca aaatattaat ggcgatggcc cctttttcgc caaatgaata
     2281 tttcacaata ccgtatgttg gcaggcgggc tttttctccc acactaatgg tgatggctga
     2341 aagtaaggtc agaatgacgc cgcctaatag tacgacgcta attaaactac cactggaaag
     2401 ttgattgccg aggcttgagg atgacaacag cacaggtgtg acggcaatac ccagcaaaat
     2461 catggtaata cgcaatcctg atgccgtatt catattatct tctttacgca taatatgttt
     2521 cctttataaa tgcgcgttac atcgtgtgaa aattcccggt gtatttgcgc cagcccccca
     2581 gtgctgtaat ctccttctgg ccttcagtca gccagctttc tgccagtacg cccagtactt
     2641 cgctaccatc ggcaagtttg atttttccaa tggtcaggcc ttcgggttcg ttcattaaca
     2701 gttcggcaaa actggccaga ggcagttgcc agatttctac gtctacgtgt gctcctcctt
     2761 cattcacgcg gatcattccg ggatgacgat cattaatact ccataaccgg taatgggcgt
     2821 cagttcggtc ttcacggaca aaaataccgc cagctttttg catatttggg ttgagttcca
     2881 gaccgcgcat cagcgttccg ttaacggcta aaagtgttgt agtcattgtg ttctccagag
     2941 aattaaaggg ttatccatgt tccgctctga tgtgccaggg ctgcttttat ggtttgcggt
     3001 gaagtaatca gtccgctggc ctgtttgcct tgttgctggc tggcattgat gaaatcaata
     3061 atggcttcca ctttgggttg catactgccg acaccaaact gaccttcctg caacagcgtt
     3121 tgagcttcgt agacgctgat agcgtcgagc cattgttgtt gtggtgtacc aaagtgaatc
     3181 gccacttttt ctacaccggt ggggatcacc agcagatcag cgcctaactg actggcgagc
     3241 aatgcggagg ccaggtcttt atcgatcacg gcttccacac ctttcagttg ttgcccgtct
     3301 cgcaccacag gtataccgcc gccgccgcag gctataacca gatatccctg gcgcacaagg
     3361 tgagcgatgg tgtggtgctc aataatttcc agtggcgcag gagagggaac tgtacgccgc
     3421 caaccacgcc cggcgtcctc catcagcgtc cagccgagtt gttgttggcg ttgttgtgct
     3481 gtttcttcat caagaaacgc gccaatcggt ttactggggc tggcgaaagc atcgtcatat
     3541 gggctgactc gcgtttgtgt caccagggca attaccggtt tgtttatgcc acggcgagcc
     3601 aattcgttat gcagcgcttt ctggaacatg tatccaatgg cgccttgggt atcacccacg
     3661 gcgtaatcca gaggaaccgg agaaacttcg ttactggcaa gttcagagcg gcgcagaata
     3721 aagcctacct gcgggccatt accatgggtt agcaccagct cccatccggc ttcaaccata
     3781 tcaacgatat gctgcacgct ttccattact gcaacatatt gatcgggaat actattgcgt
     3841 ttatcgtcct gaatgagcgc attgccgcca accgcgacga cagccagtgg cttaaccatg
     3901 atggttctcc tttgcgacga tctccgccag ttcacgtaca gcagcgacaa aacaggccat
     3961 cggtgcagtg gtgatcccgg caccaatttg tccgactccc gcttgtttgt gggcgatacc
     4021 cgtattgatg accggtaaaa tgccacgatc ggtcacttta cacgcatcaa tacctgctgc
     4081 ggtgggggca aaattgaggg ctggaagtgt aaatgcgggg ttaccgccga gggtgattgc
     4141 ctgcatccga cggctgttat tggtggcatc ggcaggggtg cctcccacaa atttaacaat
     4201 cgccggagat gacgccatcg cgaagccacc cacacctgcg gtttcagtaa ttgcgctatc
     4261 acccaggtca gaagcggcgt cgtcaacgcc aaatccgggg aagaacagac cttcaactgc
     4321 attggcagga gcctgaaacc agcgatcgcc agtaccggaa agccgaatac caaaattcac
     4381 cccgttacgc gccatcaccg taaccattga actgaacggt acattggcgg cggcatccat
     4441 cattgcttta caggcagcca tcgacaaatt gaggaagaag tgatcgttac ctgtgataaa
     4501 tgccacgacg cgctggatat gttcctgtgg aagagagcag gccagcaatg ccgggagcag
     4561 gcgcttaatc agcaaaccgg tagcggcagc attgcggtta tgaacttcat cccccatatg
     4621 cagcgcctgc gccattaatg gcttgagttc cagctcgccg tgttgtgcga ttgctgcttt
     4681 catggccggc gccagttcat cacgcatcca ggccagacga ttgagaactt catcgttatt
     4741 ggcaccaaaa cggagaacct tacccagacc ctcattgaaa ttgctaaaag tacgatggcc
     4801 gttggttttg ttttccacca cccataatgg catcgacggg ctgataatgc ctgccattgg
     4861 tccaaccgcg tgataatgat gacaaggagc aaggtcgatt tcgcctgcgt ttatctggtt
     4921 ttctgcgtct ttctggcttg tagcccagcc ttcgtacagc atggcaccaa taatcgcccc
     4981 ttttaccgga ccacacatat cttcccaggc aatcggtgga ccagagtgta aaatgcgttt
     5041 ttgttcagcc atttcgggga tggcttcttt agcgagcatg acatccacca acacgggctg
     5101 tgcttcgaga taacgtgtca tggcgatttg gttggcactt tctaccagtg gatggcgtag
     5161 taatgacgcc agaaccaaac cggtgtcaat atcaccttgt gctggcggct gccagttgag
     5221 ggatatagca tgccctcctg catgttgaat attgtcagca aaactgtgta gccccgcgtt
     5281 cacgaccttc aaaggctgat taaataaggt attcatgcat ttagctcctt tttctgcaac
     5341 tgagtctgtg caacggtgct ggcccatagc gccgcctgtg cattgctgtt ggcaatgatg
     5401 acgcctgcat tttgtagtga gcttatctgc tgactgcgta tttgcggatc agcttctgtt
     5461 ccgcaaacgt gggcaatcag caacggtgca ttattcatgt cgatgtgtga aagttggtcc
     5521 agtaattcgc tgacaggcgt agcggaggtg ccataaccaa gtacaaggtc gaataacaca
     5581 acggcagtat gtgagtcgtt gagttcgttg agcagacgtt gattgcgcag agtcgggtcg
     5641 atcattggat gcggtttgcc acgggtgaaa tcatcgtccc ccatatcaat cagtgtatga
     5701 tcttcacttt gccagatatt tgccagagca cgattacccg caactggcgt attggaggcg
     5761 gcatggaatc ccttttgttg gcagataagc tgagcttcgt aacagaaggt tcctccggcg
     5821 aaaacaccgc gtatcgcctg gcgatggact ggcagacttt ggcaggcgtt ttgtaacatt
     5881 gtgagatcgt cgcaggaaac ctcgttctcg acagccggaa gtggctgatc attcagtaac
     5941 gccacagcga tgtcggcggc gctggcaagt gtggtggcgg cagtgatatt gggacgtgcc
     6001 agatcatgcg gattcgcccc gaggaaattc accaccacgg gttttccaca agcctcggcg
     6061 cgctccagaa tagtccgggc aacgatcggt gaaggcggtt ttgaaataag cacaatcacg
     6121 tgagtttcat cgtcttgtgc cagcgcatct aaagcaaaga gcattgatat tccaccaatc
     6181 tcttcactga gatcatgtcc gcctgttccc aacgcctgag aaatacccgc tccgagctgg
     6241 tcgattcggc aagtcacttc ctgtagcccg gtgcctgacg cgccaataac accaattgct
     6301 ccgcgtttca ccacattagc gaagcctaac ggaatgccgt taacaatggc ggttccacag
     6361 tcaggtccca ttacgatgcg ctgacgttct cgggcaagcg ttttgatgta tttttcctgc
     6421 ccgatactga cgttatcgct gaacatcatc acgttcattc cgaggtttaa cgctttaatt
     6481 gcttcggcgg cggcgtagtc tcccggtaca gaaatgagtg ccaggttggc ttcgggttca
     6541 gcttccagtg ccatttccag gctgaccttt tccggtgatt taatgccaca atccgtttca
     6601 tcatcgggtt tgctggtcag acgttgctgg gccagtagca gtgcttcatt gcaaatatct
     6661 tcttcaccca tgactgcaat cactaaatca ttgggactgg cgttgatttc gttacccaga
     6721 cccgcatcgc gcaattgctc gagattattg ggtgttccca tgacgaccga ggcctgttga
     6781 ataccgggca acttgctgat ttgcgcggaa atctgcatca gagaaactga gtcctgataa
     6841 agattactga aaactttata tttaactttc atgctgttac gcctgttcca ttccgcccga
     6901 tagcaaaatt cgggcaaagc atccccgtgt agccccataa acgggctacg ggagaaaagg
     6961 ttgaaatcaa ttcatggtgt tcagggttcg aaatccatgc agcattcctg caaggcaatt
     7021 tactcctgat gttgcgccaa attgcatgat ctgttctgta caggatgcga ttgtcattgc
     7081 cgatgcgctc ccaaccagtt gtgcgagtaa ccggcaaatc ggttctgaaa aatgtccctg
     7141 aagcgcacgt tgcagataat gacgacttat atccgtggtg ttgtgtgcat gttgttcaat
     7201 tgccttctgc aacctgtatt gatgattggc aagtggtgca gggagttgcc ataaccagag
     7261 ggctgccaga tagcccagca aatagtcatc tccatcgggc gttaagcctt taccaaagcc
     7321 gatcagttgg ggtacctggc gttccagcgc atctggatgt tccataatat ctaatccctg
     7381 gtgcgtgaga ctatccggca ggtgcaatgt gctttgaacg ttatgctcca gacaaaataa
     7441 aagccgttgc gatgctatcg tggggtagtg ctggaaaacc aaaggcaatg cctcaagagt
     7501 cagcattccc cccacaaatg gcgactgcca gagcgttgcg ttatcgagcg ccacacaaaa
     7561 tcgaggtgtc cgcagtaagc ctggggttaa ttgaatggga tctgaatggg caatctctct
     7621 tcgccagtcc caacattcag gtagccagac tctggcagca tcagggagat tcagatacgt
     7681 ttgcgcacac aacagagtga tgaatgttcc cgcgtctgaa tacagattta ccgcatgttt
     7741 aaaagcgcta tgtagacgta aatcttgcgt ttcattctgc gggaaaagat atcccaacga
     7801 cgatgcacaa agcgtaacct gtaccatcgg cgttagcctt ccagacctgc aataatggct
     7861 ttggaatcag taacccaacc gaaaatggca ccttgtgctt tgatcatctc aagggcgtat
     7921 ttctggaatt ccgggaaata ggagccaacg caatcttccg gtatgatgca ttcatagcca
     7981 cggtcatttg cttcacgcac ggtggtggtt acgcaaactt cggtagtcac gccacaaaca
     8041 ataagtgtct tgataccacg cttttgcaaa atcagatgta agtcggtttg ataaaaagcg
     8101 cctttaccgg gtttatcaat cacaggttcg cctgtcaccg gatacagttc aggaatgatg
     8161 tcatgacctg cttcacccct gaccagaatg cgtcccatcg gtcctggttc accaataaag
     8221 gtttttccgc cgcgcgtcag tttggctgat gggcagtcgc ttaagtcttc acgatggcct
     8281 tcacgggtat gaataaccat aattcctttt tgacgtgcgg ctgccagaac ttctttacat
     8341 ggtgcgatag ccgtgcgaac cagtgaaaca tcattaccca atgcttctcc aaaccctcct
     8401 gcttcgacaa aatcacgttg catgtctatc atgatcagtg ccgttgtgca cggatcaaag
     8461 ggtaattcaa agggttgagc ctgaaaaatg gatatcgtca tgacaaatct ccagtatata
     8521 ttgtttactg gtgacgagta ttcaatataa aggtgaccca caacagaaaa aaagttgttt
     8581 tacaggagcg aatatttttg aggtgtctaa atgttgttat gaaaaatgtg atttacatca
     8641 atctatgaca t
//
LOCUS       DAJOWB010000167         8635 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJOWB010000167 DAJOWB010000000
VERSION     DAJOWB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.167"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            97..813
                     /gene="nanC"
                     /locus_tag="OHO85_004309"
     CDS             97..813
                     /gene="nanC"
                     /locus_tag="OHO85_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCQ2673585.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            833..1939
                     /gene="nanM"
                     /locus_tag="OHO85_004310"
     CDS             833..1939
                     /gene="nanM"
                     /locus_tag="OHO85_004310"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCQ2673586.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2004..2984
                     /locus_tag="OHO85_004311"
     CDS             2004..2984
                     /locus_tag="OHO85_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCQ2673587.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALVKNPQNKFLGVCWMQGEFDLMTSDYVSHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDSFVEAILQFWRER"
     gene            complement(3567..4806)
                     /locus_tag="OHO85_004312"
                     /pseudo
     CDS             complement(3567..4806)
                     /locus_tag="OHO85_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378101.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            4922..5167
                     /locus_tag="OHO85_004313"
                     /pseudo
     CDS             4922..5167
                     /locus_tag="OHO85_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420158.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            5544..5801
                     /locus_tag="OHO85_004314"
     CDS             5544..5801
                     /locus_tag="OHO85_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673588.1"
                     /translation="MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLT
                     DCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRAQLDNR"
     gene            5813..6358
                     /locus_tag="OHO85_004315"
     CDS             5813..6358
                     /locus_tag="OHO85_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCQ2673589.1"
                     /translation="MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDES
                     ARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMHILAPLAVIPEYQGMGVGGRLIR
                     TGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
                     QPMTLTGHIRCADPDETGALT"
     gene            6414..7160
                     /locus_tag="OHO85_004316"
     CDS             6414..7160
                     /locus_tag="OHO85_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCQ2673590.1"
                     /translation="MDIPRIFTISESEHRIHNPFTEEKYATLGRVLRMKPGTRILDLG
                     SGSGEMLCTWARDHGITGTGIDMSSLFTAQAKRRAEELGVSERVHFIHNDAAGYVANE
                     KCDVAACVGATWIAGGFAGAEELLAQSLKPGGIMLIGEPYWRQLPATEEIAQACGVSS
                     TSDFLTLPGLVGAFDDLGYDVVEMVLADQEGWDRYEAAKWLTMRRWLEANPDDDFAAE
                     VRAELNIAPKRYVTYARECFGWGVFALIAR"
     gene            7329..7547
                     /locus_tag="OHO85_004317"
                     /pseudo
     CDS             7329..7547
                     /locus_tag="OHO85_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348444.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7585..7701
                     /locus_tag="OHO85_004318"
                     /pseudo
     CDS             7585..7701
                     /locus_tag="OHO85_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227376.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            7946..>8635
                     /locus_tag="OHO85_004319"
     CDS             7946..>8635
                     /locus_tag="OHO85_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family peptidase"
                     /protein_id="HCQ2673591.1"
                     /translation="MSFSVQETLFSLLQHNAISGHENAVADVMLCEFRRQAKEVWRDR
                     LGNVVARYGSDKPDALRLMIFAHMDEVGFMVRKIEPSGFLRFERVGGPAQVTMAGSIV
                     TLTGDKGPVMGCIGIKSYHFAKGDERTQSPSVDKLWIDIGAKDKDDAIRMGIQVGTPV
                     TLYNPPQLLANDLVCSKALDDRLGCTALLGVADAISTMELDIAVYLVASVQEEFNIRG
                     IVPVLRRVKPDL"
BASE COUNT         2342 a         2042 c         2024 g         2227 t
ORIGIN      
        1 actataagcc tggcaaaaaa atattacaca acataaatgc taattgttta tgcgggcttt
       61 gtattgcttt ctgtatccta caaatgagtg aaatttatga aaaaggctaa aatactttct
      121 ggcgtattat tactgtgctt ttcgtcccca ttaatttctc aggctgcgac actggacgta
      181 cgtggtggat atcgtagtgg aagccacgcc tatgagactc gactcaaagt cagtgaggga
      241 tggcaaaatg gatggtgggc aagcatggaa agtaatacct ggaataccat tcatgataat
      301 aaaaaggaaa atgccgcact caatgatgtt caggttgaag ttaattacgc aattaaactt
      361 gatgatcaat ggacggtgcg cccgggaatg ttaacgcatt ttagcagcaa cggcacacgc
      421 tacggaccct acgtaaaact gtcctgggac gcgacaaaag atcttaattt tggcattcgc
      481 tatcgttacg actggaaagc ttaccgacaa caagacttat ccggtgatat gtctcgtgat
      541 aacgttcatc gttgggatgg atatgtcact taccatatta atagtgattt caccttcgca
      601 tggcaaacga cgctatacag caaacagaac gattatcgct atgcaaacca taagaaatgg
      661 gcgacggaaa atgcatttgt tctacaatac catatgacgc ccgatattac gccatacata
      721 gaatatgact acctggaccg tcagggtgtt tacaatggca gagataattt atcggaaaac
      781 agttatcgca ttggtgtgtc atttaaactg tagtagacag gagacagtca caatgaataa
      841 aacaataacg gcgcttgcta tcatgatggc ttcatttgcc gcaaacgcgt ctgtattacc
      901 ggaaactcct gtgccattta aaagtggtac cggagcaatt gataacgaca ctgtctacat
      961 tggtttaggt agcgcaggta cggcatggta caagctggat acacaggcca aagataaaaa
     1021 atggacagcg ttagctgcat tccctggcgg accaagagat caagcaacct ctgcatttat
     1081 tgatggcaat ctgtatgtgt ttggcggcat tggcaaaaac agcgagggct tgactcaggt
     1141 atttaatgac gtacacaaat acaaccccaa aaccaatagt tgggttaaat tgatgtcgca
     1201 cgcgccgatg ggcatggcgg gccatgtgac ttttgtacac aacggcaagg cttatgttac
     1261 tggtggtgtt aaccagaata tcttcaatgg ctattttgaa gatctcaacg aggctggaaa
     1321 agattcaacc gctatagata aaatcaacgc tcactatttt gacaaaaaag cagaagatta
     1381 tttcttcaat aagtttctgt tgtcttttga tccctcaaca cagcaatgga gttacgctgg
     1441 cgaatcgccc tggtacggaa cggctggtgc ggcggttgtg aataaaggtg ataaaacctg
     1501 gcttattaat ggcgaagcca aaccaggatt gcgaacggat gccgtatttg aacttgattt
     1561 caccggtaat aatttaaaat ggaataagct tgctcccgtc tcatcaccag atggcgtcgc
     1621 tggcggtttt gcggggataa gcaatgattc tcttatattt gccggagggg ccggattcaa
     1681 aggttcacga gaaaattacc agaacggtaa gaactatgcg catgaaggcc tgaaaaaatc
     1741 atatagcact gatattcatc tttggcataa cgggaaatgg gataaatcgg gtgaattatc
     1801 gcaaggtcgg gcctacggag tatcattgcc ctggaataat agtctattga ttattggcgg
     1861 tgaaactgca ggcggcaaag cggtgacgga ttcagttttg atctctgtga aggataataa
     1921 agttacagta caaaactaac gcttcagggc cccggtaaag gggccctgct atcaatttgt
     1981 catttcaatt acgcgaaatt tatatgaacg caataatatc gcccgattat tactatgttc
     2041 ttaccgttgc tggtcagtct aatgccatgg cgtatggcga aggactgcca ttaccggaca
     2101 gggaagatgc gcctcatccc agaattaaac aattagcgag atttgcgcat acgcatcccg
     2161 gaggcccgtc atgtcacttt aacgacatta ttccactgac tcactgccca cacgatgttc
     2221 aggatatgca gggttatcac catcctctgg caacgaatca tcaaacccag tacggcaccg
     2281 ttggccaggc actgcatatt gcacggaaat tactgccctt cattcctgat aatgcaggga
     2341 ttctcatcgt tccgtgttgc cgtggcggat cggcttttac cgcaggcagc gaagggacat
     2401 attcagaacg gcacggagcc agccatgatg cttgtcgttg gggaacggat actccgctat
     2461 accaggattt agtcagcaga acgcgagccg cactggtaaa aaacccgcag aacaaattcc
     2521 tcggcgtatg ctggatgcaa ggcgaatttg acttaatgac cagtgactac gtgtcacacc
     2581 ctcaacactt taatcatatg gttgaagcct ttcgtaggga tctaaaacaa taccattctc
     2641 agcttaataa tattactgac gcgccgtggt tttgcggcga taccacctgg tactggaaag
     2701 aaaatttccc tcatgcgtat gaagctattt atggcaatta tcaaaataat gttttagcca
     2761 atattatttt cgtcgacttc cagcaacaag gtgaaagagg actgacgaac gcgcctgatg
     2821 aagatccgga cgatttaagc acgggatatt acggttcagc gtaccggtca ccggagaact
     2881 ggacgacggc actacgaagc agtcatttca gcgcggcagc ccgtcggggg attatttctg
     2941 acagttttgt agaagcaatt ttgcagtttt ggcgcgaaag gtgagcgggg agtttattca
     3001 tgggggatgt ccttgttcgt tctcagagta gacaacaaaa aacaaatgag atcaaggtaa
     3061 tatctcttca cttgcgggct cgaacagcag gcaattgcca atataataat ctggcacaag
     3121 ggtgaaaaac gcgcaccgca taaaccgttg ttattgctta cagattagcc ggatgcctta
     3181 atagacatcc gcgccttttc gattatggct cggaaatcta cgaacatcta cacagtttac
     3241 tggaacgttt tgggccacag cgttcgcagc accgtcctga tatgccgttc tggcgattat
     3301 aagtcgacgg attctggcaa atagacgatg atgatatgct taatgatgtt caaagaaatt
     3361 aggccattat tatattattg cttgcatttt ctttcattcc taaaagcacc cgccattaat
     3421 cccaggcgca ttatgcaaag gaaaatgatt aggtaaacaa taagatttat aatcaggaaa
     3481 ggcgactcgc aataaaacgg aaagtcgcct taacattggt aaaatatgcc ctttactact
     3541 cctgtatcat gaacggctca tcacatttaa agctgacgtt gatccagact accatatatt
     3601 gcctttattt cattttttac gccttcacca cggtacacca aagacgtatc gtatcgttga
     3661 tacttatctt cccgtgtagc actgaaccag ttaaaagatc cgacacacag cagttcttca
     3721 tcctcaatca caattttgct gtgaacacga ttaaccaatt tcgtagcgat acccatctta
     3781 ttgagctttt caacagcatc attcagcaga tgttgtttct cctgacgctt atcatcatcg
     3841 acatgcgcta tattgcagtt tttatccgtc accacggtaa tatcaatgcc ccgggacggg
     3901 ccaaagccat tgatgccaga aatcccgttt gctccacttt ttgccaggat aaccaagggg
     3961 aaataatcgt tattttcttt tgagctccgg caagagtctt gttcaaaaat tcatcatgct
     4021 gctccacacc atgcagcgtt gaaatttgtg tatgtgcgga aattaaatcc tgtcgtttct
     4081 gaaactcaaa ttgcaacgca ttattatctg aagaaaagag atattttgcc aataaccctc
     4141 gcggcgaaaa tgcgggctgc atctcgataa ggtccatatc gccaaatacc agaaaactat
     4201 ctttagcgcg tgagacagca acattgagga tagtgctgtt gctatcaaga aacctgccat
     4261 cttcatgttt tgagtagaca ggagaaaaga gaacaatcgc cctttccgcc ccctgcaagg
     4321 agtgcacagt ccccactgtc agtaaaccct gctcgtcttt gccattaatt tccagcttac
     4381 gcaatgacat tttgatagca ttaacctgcg ccgaaaatgg cgtcacaaca ccaacaactt
     4441 tgtacagtgg ttccccgtaa tggcgctcta tatcatcctt atgtgccacc agccatgctg
     4501 ctattgtttc agcttcaaga gggttatgac ggctccctcc atttggcttc atgcctctac
     4561 catcaatatg taaatatccc attgcgggga aaatcgtcct ttttccatcc ctcttttagg
     4621 ctgcaactta ccgtgatagc aaagtgagtt acagtagtcg atgatgttat tgaaacagcg
     4681 acgatgctca tacagataca ttccgcgctc gaacttggga tcatactggt agtgagagtt
     4741 gaactgggct acctgcatta cgctgccaga tgctgcactt tttccagacc catttattat
     4801 cgaatacggt gaccgcctga gcggccacct ttaatcgtgg gcagttacac agaattattt
     4861 acagggtctt gctgggagtt cttacacttg ctcgcgatat tcaattggcc attgtcaaca
     4921 aatgacgcag gttttgattg gtgatatcta tttacgtcag caatgcgatg tgttttggct
     4981 cggctatacc atcagtcccg cttatgctcg ccagggatat gcaattgaag caattaccgc
     5041 cacgattgac tgaataaaag agaacggttt ttcactcatc aaagccggtg tgaatccgga
     5101 aaataccctt tcaaaaaaat tacttatcag gattggcttc aatttttcaa gcatcgaaga
     5161 tgggtgagca tatttatgtt ctggatttaa caaaaggaga ataatctccc gaagggaaaa
     5221 gccgatgagg ccatttcagg cgtacttcaa catgcattcg tcagtgtcat ctgccttttc
     5281 cagtaaatgg gttggcattc aatttccaaa gtacatcaca ctttttctac tgaaacatat
     5341 ccctcctata actacgtgtt aatattcgct ccgtaaatac ctttcaactc aacccgaggc
     5401 tttgatgctg gagaatgtaa tcatccgata atcagttccc cgaccttttc aggccggact
     5461 gattatcaat gcgccgaaat cgaatgcgga caccgcggtg tgtttgcacg ttttgcacat
     5521 gatgattaaa taggttcttc agtatgaatt tatcccgtca ggaacaacat accttacacg
     5581 ttctcgctaa aggtagacgt attgcgcacg tccgcgattc ttcaggccgc gtcacttccg
     5641 ttgaatgcta cagccgcgaa gggctgttgc tgaccgactg cacgctcgcc gtcttcaaaa
     5701 aactcaaaac caaaaaactt atcaagtccg tcaatggcca gccctatcga atcaacacca
     5761 ccgagctgaa taaagttcgc gctcagctcg ataatcgcta aggagagttt ctatgacagt
     5821 acaccacttc acttttcata tcacagataa aagtgatgcc agtgatatcc gggaagtcga
     5881 aacccgcgca tttggcttca gcaaagaagc cgatttagtg gcctctttac ttgaggatga
     5941 aagcgcgcgc cccgcgttat cgctgctagc ccggtatgaa ggcaaagccg tcgggcatat
     6001 tttatttacc cgggcaacct tcaaaggaga aatggattca ccgttgatgc acatcctcgc
     6061 tcccctggcg gtcataccgg aataccaagg gatgggcgtg ggaggacggt tgatacgcac
     6121 tggcatcgag cacctgaggt tgatgggctg ccagacggtt tttgttctcg gccacgccac
     6181 ctattacccc cgacatgggt tcgaaccctg tgccggagac aaaggctatc cggcacctta
     6241 tcccatcccg gaggagcaca aagcctgctg gatgatgcaa tcactcaccg ctcagcccat
     6301 gaccctgacc ggccacatcc ggtgcgcaga ccctgatgaa accggagcac tgacgtgaat
     6361 aacaatcttg ttgtgtcctt tttaaggctt aacccaataa caggaattaa ataatggata
     6421 tcccacgtat ttttaccatc agcgaaagtg aacaccgcat ccataacccg ttcaccgaag
     6481 agaagtacgc cacgctgggt cgcgtgctgc gcatgaagcc gggcacccgc attcttgacc
     6541 tcggcagcgg ctcgggcgag atgctctgca cctgggccag ggatcatggc attacgggga
     6601 ctggcatcga catgagttcg ctcttcaccg cgcaggcaaa gcgtcgcgcg gaagaactcg
     6661 gcgtcagcga acgcgtacat tttattcata acgatgcggc cggttacgtc gcaaatgaaa
     6721 aatgtgacgt ggcggcctgc gtcggcgcga catggattgc cggtgggttt gccggggcgg
     6781 aggagctgct agcgcaaagc ctgaaaccgg gcgggatcat gcttatcggc gaaccctact
     6841 ggcgtcagct accagcaaca gaagaaatag cccaggcctg cggcgtcagc tcgacaagcg
     6901 atttcctgac gctgccagga cttgtcggag cgttcgacga tctcggctat gacgtggtgg
     6961 aaatggtact ggcagaccag gaaggctggg accggtatga agccgcaaaa tggctgacca
     7021 tgcgccgctg gctggaagcg aatcctgacg atgacttcgc cgccgaagtc agggccgaac
     7081 taaacatcgc gccgaaacgc tacgtgacct acgcgcggga atgctttggc tggggcgtgt
     7141 tcgcactcat cgcccggtaa caacaggttc ccggtatttc tggctggaaa tatcgaggct
     7201 ttacctttgc tttgatctga tgtatcacct gcacaataca tattcagtat gtttattaat
     7261 caagatgtca ccataaggag catcatatgt cacaacatga acagttctgt caggcttgcg
     7321 gaatgccgat gtcggcacca gatgcacaca gtgccagtga tcaatattgt gcgtaatgca
     7381 gcgacagcaa cggcaatctc aaaccctggg aagaagcggt atcaggttta gccagcttcc
     7441 ttgattcctg gcaaaaagtc ggcccggagg aagcacataa gcgtgcaaaa cgttatctga
     7501 cggcaatgcc agcctgggct cataaagcgg agagtgattt gagctgatta tctatactcc
     7561 ctctgaatct attcaggaga tccgatgttt tcttacatta tgctgggaac caacgacctg
     7621 ccacgcgcca ttacattcta agatccgctg atggagatgc tggggtatcc acgtgcagga
     7681 cgtaatgaag agggagcgtc ctgcaacaaa gtggtgctat aacagttacc gcgttttatt
     7741 catcctcatt atttcaacca taatcactgg tctctccagc ccgtaacttt cacaaacgtt
     7801 gagcgctttt attccactcg gctcacataa ttttttattt gttcagcttt gcgcgttttc
     7861 acgtgaacgt tctcacaatt attcgaccag tctgcgccta taaaacgctc aacgagtgat
     7921 tgttttacgg tctacggaga tagctatgtc attttctgtg caggaaaccc tgttctcgct
     7981 tttgcagcac aatgcgattt caggacacga aaacgctgtc gctgacgtca tgctgtgcga
     8041 attcaggcgt caggcaaaag aggtctggcg agacaggctt gggaatgtcg tcgcgcgcta
     8101 cggtagtgat aaacccgatg cgctgcgact gatgattttt gcgcatatgg atgaagtcgg
     8161 ttttatggtg cgcaaaatag agccgtctgg atttttacgc tttgaacgcg taggcggtcc
     8221 tgcgcaggtc actatggctg gttccatcgt caccctcacc ggggacaaag ggccagtcat
     8281 ggggtgtatc ggcattaagt cctaccactt tgccaaaggc gacgagcgca cgcagtcacc
     8341 ttctgtcgac aaactgtgga ttgatattgg tgccaaagac aaagacgacg ctatacggat
     8401 gggcattcag gtcggtacgc ctgtcactct gtataacccg ccgcaactcc tggcaaacga
     8461 tctggtgtgc agtaaagcac tagacgatcg tctaggctgt actgccctgc tcggtgtagc
     8521 ggatgctatc agtactatgg agcttgatat cgccgtttat ctggtggctt cggtacagga
     8581 agaatttaat atccgcggca ttgttcccgt attacgccgt gtaaaacctg acctg
//
LOCUS       DAJOWB010000168         8634 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJOWB010000168 DAJOWB010000000
VERSION     DAJOWB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.168"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1501
                     /locus_tag="OHO85_004320"
     CDS             <1..1501
                     /locus_tag="OHO85_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCQ2673592.1"
                     /translation="LSELFYKELEDKFSGKELATPLLKSFSENCRQNGRHIFSNKDFV
                     IKFSTSVLQTDKKEITIINKNENTTLTQTIAPIFEEYLMEILPQRSDALDKQKLNLNS
                     DRKEKDFPRVKLNGQCYFPGRPQNRIVCRHIAAQYINDIYQNVNYKPHQDDYSSAEKF
                     LTHFNKKCKNQTLALISSRPEGRCVAACGDFGLVMKAYFDKMESNGISVMAAILLVDN
                     HALTARLRIKNTTEGCTHYVVSVYDPNVTNDKIRIMSESKEDIKHYSLMDFMNVDYSL
                     LKWSNDHVINQSVAIIPALPKEQLLMLKGSVDEITPPLSPATMNLLMAIGQNHQLTQL
                     MIQLQKMPELHRTEMLTAYNSGHMNVINTIFNALPTLFNTFKFDKKNMKPLLLANNSN
                     EYPGLFSAIQHKQQNVVETVYLALSDHARLFGFTAEDIMDFWQHKAPQKYSAFELAFE
                     FGHRVIAELILNTLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(1470..3206)
                     /gene="aceK"
                     /locus_tag="OHO85_004321"
     CDS             complement(1470..3206)
                     /gene="aceK"
                     /locus_tag="OHO85_004321"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCQ2673593.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3389..4693)
                     /gene="aceA"
                     /locus_tag="OHO85_004322"
     CDS             complement(3389..4693)
                     /gene="aceA"
                     /locus_tag="OHO85_004322"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCQ2673594.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4723..6324)
                     /gene="aceB"
                     /locus_tag="OHO85_004323"
     CDS             complement(4723..6324)
                     /gene="aceB"
                     /locus_tag="OHO85_004323"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCQ2673595.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6593..7522)
                     /gene="metA"
                     /locus_tag="OHO85_004324"
     CDS             complement(6593..7522)
                     /gene="metA"
                     /locus_tag="OHO85_004324"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCQ2673596.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7679..8122
                     /locus_tag="OHO85_004325"
     CDS             7679..8122
                     /locus_tag="OHO85_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCQ2673597.1"
                     /translation="MVISIRRSRHEEGEGLVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGA"
     gene            complement(8185..8568)
                     /locus_tag="OHO85_004326"
     CDS             complement(8185..8568)
                     /locus_tag="OHO85_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673598.1"
                     /translation="MSVLYIQISRNKITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLHDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSVVLSDSAVMAMLKQK"
BASE COUNT         2231 a         2119 c         2107 g         2177 t
ORIGIN      
        1 cctgagtgaa ttgttttata aagaactcga ggataaattt tctggcaagg agctggcgac
       61 gcctctatta aaaagcttct cagagaactg tagacaaaac ggtcgtcata tctttagcaa
      121 caaggatttt gtcattaaat tttccacgtc tgtcttacag actgataaaa aagaaattac
      181 gataattaat aaaaatgaaa acacgacact cactcaaacc attgctccaa tatttgaaga
      241 atacctaatg gaaattttac ctcaacgctc agacgctctt gataaacaaa aattaaatct
      301 aaactcagat agaaaagaaa aagacttccc aagagttaaa cttaatggtc aatgttattt
      361 tccggggcgc ccccaaaacc gtatagtatg ccgacacatt gctgcacaat atattaatga
      421 tatttatcag aatgttaatt acaaacccca tcaagatgat tactcttcag ctgaaaaatt
      481 tctcacgcac ttcaacaaaa aatgcaaaaa ccagactttg gcgttgattt ccagccgtcc
      541 tgaggggcgt tgtgttgctg cctgcggtga tttcgggcta gttatgaagg catattttga
      601 caagatggaa tcaaatggca tcagtgttat ggctgccata ttattggtag ataaccatgc
      661 tttgacggcc cgactaagaa taaagaacac aactgaagga tgtacccatt acgtggtttc
      721 ggtttatgat cctaatgtaa ctaacgataa aataagaatt atgagcgaaa gcaaagagga
      781 tattaaacac tattctctga tggattttat gaatgtagat tatagcctcc tgaaatggtc
      841 aaatgatcat gttattaacc aatctgttgc aataattcca gcacttccga aagaacagct
      901 attgatgtta aaaggatctg tggatgaaat aacccctcca ttatcaccag caacgatgaa
      961 tttgctaatg gcaattggtc agaatcacca acttacgcaa ctgatgattc agctccagaa
     1021 aatgccagaa ctacatagaa cagaaatgtt gactgcctat aatagtggac atatgaacgt
     1081 tattaatact atttttaacg cattacccac tctgtttaat acgtttaaat tcgataaaaa
     1141 aaatatgaag cccctcctcc tggcaaataa ttctaatgaa tatcccggtt tgttttcagc
     1201 gatacagcat aaacaacaaa atgttgtaga gacggtttat cttgctttat ctgaccatgc
     1261 acgcctgttt ggatttaccg ctgaagatat tatggatttt tggcaacaca aagcgccaca
     1321 aaaatactct gcctttgagt tggcttttga atttggtcac cgggttattg ctgaattaat
     1381 ccttaataca ttaaataaga tggctgaaag ctttggcttt acggataacc ctcgatacat
     1441 tgcggagaaa aattatatgg aagctttact caaaaaagca tctccccata ccgtacgcta
     1501 aatctttgcc tgcgccgata cgcataaaca tcttccacat gcccttcacg tatgcggttt
     1561 tgtagtgcgc gccagtaatc agcgcggaac aggtcggcgt gcatctcttc aaacagcgga
     1621 ccaatacgcg ggtcggcgca tagccagtgg cgaaactctt ccgggaaaac atcgcccggc
     1681 gagacgctgt accacggttc gctggcaagt tcgtcttccg gatagcgcgg cggcgggatg
     1741 tcgcggaaat tcacttccgt catgtagcaa atttcatcgt aatcataaaa aaccacacgc
     1801 ccgtgacggg tgacaccgaa gtttttaaac agcatgtcgc caggaaaaat gttagcagcg
     1861 gcaagctggc gaatagcgtt accgtattct tcaatggcgt cgcgcaactg ctgaccttcc
     1921 acttgttcca gccagatatt gagcggcacc atccgccgct cgatatagag atggcgaatt
     1981 acaatctgct caccaagatc ggtgattttt tccgctgctt cctggagcag taattccatt
     2041 aatgccgggg aaatatgccg cttctccagc acaaagtttt caaactcctg ggtgtccgcc
     2101 attcggccca cgcgatcgtg ctctttcacc agttgatagc aggcacgaac gtgagcggca
     2161 gacatctctt tctgcggcgc gaacttgtct ttgatgactt tgaatacccg atcaaagccc
     2221 ggcagcgtaa acaccaacat caccattcca cgaatacccg gcgcttcaat gaactgctca
     2281 ttacagccct gtagataaac gagatattcg cggtagcttt cggttttggc gtgcttctgg
     2341 cagccgatag ccatatacaa ttcagcagtg gttttacctg gcagaatttc ccgtagccac
     2401 tcgaccagcg ctgcgggcag cggtgcataa accataaaat aagaacgcgc aaagccaaaa
     2461 acaatgctcg cttcggcggt cgtcgtcagg caggtatcaa taaataactc gccgtcgtcc
     2521 gtctggtgga tcggcagtaa gaagggcaac gtgccggaag gcgtgatcag tttgcccacc
     2581 agccaggcgg ctttattgcg gtaaaacagt tcgttcgcca cctgtaaatg actttccgcg
     2641 aggttgtctg tccccagcgt ttccgtcaga tggcgaatga tgtaatgaat gtcacggctt
     2701 ttattctgcc agcgcaggcg cagcggcagg tcgctgataa cgcgcatcag tagagattcc
     2761 cagccgtgat cggggtgaaa gtctttcgcc agcgggcggg gaatggtacg aaagcggcgc
     2821 tctggctgag agctaaagat aaaaagccgc tcgggagtaa gcgagcggtg gtcaaataac
     2881 cgacagtaca cggagttaaa aaagctctcc gcaatctcga agcgcgggta atccggcaac
     2941 agccgcgtgt aatgctcttt aacgcgcagt aaaaatgccg cgtccgtact ttggccgtta
     3001 gtaatgcagc gcagttgctc cacgaccaga ccaacgtgat gatcgtaaag atggatacgg
     3061 tttttcatcg cctgctggac agcatgccag tcggcctgtt cgaaacgctg ctgcgccccg
     3121 gaagtcactt cgaggaatcg accatactga gcgtcgaagc cttgcaaaat ggtttgagca
     3181 atcagtaatt ccaggccacg cggcatttag cgccctcatc aggagcagag aattgccgga
     3241 tgcggcgtaa acgccttatc cagcctacgt tcggcaacgg ttgtaggcat gataagacgc
     3301 gcaagcgtcg catcaggcat cgtgcaccga ttgccggatg cggcgtgaac gccttatccg
     3361 gcctacagtc agcaacggtt gttgttgctt agaactgcga ttcttcagtg gagccggtca
     3421 gcgcggtgac tgaagacgtg ccgccctgaa taatagtcgt cactttatcg aagtaacctg
     3481 tacccacttc ctgctggtga gatacgaagg tatagccatc tttcgcggcg gcaaattccg
     3541 gctgctgcac tttctcaacg tagtgcttca taccctcgcc ctgggcatag gcgtttgcca
     3601 ggtcaaacat gttgaaccac atgctgtgga tacctgccag ggtgatgaac tggaacttgt
     3661 agcccatatc cgacagctgc tgctggaagc tggcaatagt tttgtcgtcg aggtttttct
     3721 gccagttgaa cgacggcgag cagttataag ccagcagttt gcccggatat ttcgcgtgga
     3781 tagcttgtgc aaagcgacgc gccagttcca gatccggcgt ggaggtttca caccagacca
     3841 ggtcagcata tggcgcatac gccaggccac ggctgatcgc ttgctcaatg cccgcatgag
     3901 tacggaagaa gccttcactg gtacgctcgc cagtaataaa ttcactgtca tacgggtcgc
     3961 aatcggaggt aatcagatcc gccgcatcgg catcggtacg agcaaccagc agggttggaa
     4021 cgcccgtcac gtcagctgca agacgcgccg cgaccagttt ctgaatagct tcctgagttg
     4081 gcaccaaaac tttgccgccc atgtgaccgc atttcttcac tgacgccagc tgatcttcga
     4141 agtgaactgc cgctgcaccg gcttcaatca tcgctttcat cagttcaaag gcattcagga
     4201 caccgccaaa accggcttcc gcatcggcaa cgatcggcag gaagtaatcg acatagcgtg
     4261 gatcgcccgg ctcaatgcct gcggaccatt ggatctgatc ggcacgacgg aaggtgttgt
     4321 tgatccgctc caccacagct ggcaccgagt ttgccggata gagcgactga tccggataca
     4381 tgctggccgc caggttagcg tccgccgcta cctgccatcc cgacagatag actgcttcaa
     4441 tacccgcttt cgcctgttgc agcgcctgac cgccagtcag tgcaccgagg ctgttgatgt
     4501 agcctttttt cgactcgcca tgcagcagac gccacatttt cgccgcgccc agttgcgcca
     4561 gggtgcattc aggattgact gaaccgcgta atttcaccac atcttccgcg ctgtatgggc
     4621 gagtaatgcc ttcccaacgc ggttgagtcc actctttctg taattcttca atttgttgtg
     4681 tacgggtttt catgtgcaga tgctccatag ttatgtggtg gtttacgcta acaggcggta
     4741 gcctggcagg gtcaggaaat caattaactc atcggaagtg gtgatctgtt ccatcaagcg
     4801 tgcggcatcg tcaaaacgcc cctgggagaa acgttcttcg cccagttcgc tggcaatgac
     4861 tttcatctct tcacccagca tctggcggaa caaggctttg gtcaccggtt tgccattgct
     4921 caacgttttt tgatgatgga tccactgcca gattgaggta cgggaaattt cagccgtcgc
     4981 cgcatcttcc atcaggccat aaatcggcac gcagccgttg ccggagatcc atgcttcgat
     5041 gtactgcact gccacgcgaa tgttggcgcg cataccttct tcggtgcgtt caccatcaca
     5101 aggtgccagc agctgatcgg cagtaatcgg cgcgtcttgt tcgcgcatca cttcaagctg
     5161 atttttacgg gagccgagaa tgtcgttgaa taccgccatt gccgtatccg caaggcctgg
     5221 gtgagcaatc catgtgccat cgtgaccgtt attggcttcc agcgctttat ccgcttttac
     5281 tttgttgagc acctggttat tgcgctcttc atctttgctc ggaataaacg ctgccatgcc
     5341 gcccatcgca aaagcaccgc gtttatggca ggttttaatc aacaggcgtg agtaagcatt
     5401 caggaatggt ttatccatcg tcactgcctg tctgtctggc aggacgcgat cgggatagtt
     5461 tttcaacgtt ttgatatagc tgaagatgta atcccaacga ccgcagttca gaccaacaat
     5521 atggtcacgc agcgcgtgaa ggatttcatc catctggaac acggcgggca gcgtttcaat
     5581 cagcaacgtc gccttgatgg tgccgcgcgg cagattaaag cgatcttctg catagctgaa
     5641 gacttcgctc caccaggccg cttcctgcca ggactgggtt ttcggcagat agaaataggg
     5701 accgctgccc tttgccaaca gtgcctgata gttgtggaag aaatagagcg caaaatcaaa
     5761 caggctaccg gggattgcct caccacgcca ggtgacatgt ttttccggca agtgcagacc
     5821 gcgtacccga caaatcaaaa ccgctggatt gggcttgagc tgataaattt tgcctgcttc
     5881 attggtatag ctgatggtgc cgttaaccgc atcgcgcagg ttaatttgcc cgtcgatcac
     5941 tttgttccag tctggtgcca gtgaatcttc gaaatcggcc ataaagactt tcacattggc
     6001 gttcagtgcg ttgatcacca tcttgcgctc aaccgggcca gttatctcga ctcgacgatc
     6061 ttgtaagtcc gcgggaatac cacgaatttt ccagtccgta tcgcgaatgg aagctgtttc
     6121 cgaaataaaa tcaggcaacg ttccgttatc aatatcttgc tgctgctgaa tgcgcgctgc
     6181 cagaagttta ttgcgttgtg gcgtaaaatg cgtcaccagc tcagtcagaa attctaccgc
     6241 ttcggcagta agaatttgct tctcctgctc gccatacggc cttatgaaag ccagttcatc
     6301 ggttgttgtt gcctgttcag tcatcgtgca gctcctcgtc atggatccga aaacttcccc
     6361 actgtgaacg aaggatcgtt gtgcgctttt cgttcagcac aactaagact actcatttaa
     6421 aatgcaaaat caaaaacaat ttccatttta attaaaaata cttgataact tattgataac
     6481 aatatgaata aaatttattc acctcctgag gtgcgtttcg gaaataaaaa aggcacccga
     6541 aggtgcctga ggtaaggtgc tgaatcgctt aacgatcgac tatcacagaa gattaatcca
     6601 gcgttggatt catgtgccgt agatcgtatg gcgtgatctg atagacgtaa tagttgagcc
     6661 agttggtaaa cagcaaatta ccgtgactac gccagctcgc tcgcggtgta ttttgcggat
     6721 cattgtgcgg gaaatagtta tacggtactt ccgggcctag tccggcttcc acatcgcgga
     6781 aatattcctg cgccagcgtt tgcgcatcat attcgggatg gcccgtcaca aaggcaatgc
     6841 gcttatcttt gctggcaaac agatatgcat ccccttcttc cgtctctgcc agaatttcca
     6901 gatcggtgta atcacgaatc aacgctgccg gaaagtcagc atagcgcgaa tgtggtgcca
     6961 ggaatgaatc atcaaagcca cgcgtcagaa gcgcatgagg atggagaata tgatgctcgt
     7021 aaacgccaga gagtttgtcg gtgcgagttt gcttaggaat gccgtagagg atattgagcg
     7081 cggcctgtac cgcccagcag acaaacagcg tcgaggtgac gtgatctttc gaccactcca
     7141 gcacctgttt gatctgcggc cagtaagcga catcattaaa ctccaccagg cccagcggcg
     7201 caccggttac aatcagaccg tcaaagttct gttcctgaat atcttcaaag ttacagtaga
     7261 agttgttcag atgctctgcg ggcgtgttgc gcgattcacg ggaatcgatg cgcaacagct
     7321 gaatatcgac ctgcaaaggt gagtttgaaa gcaggcgcag aaactgattt tcagtttcaa
     7381 tcttcttcgg catcaggtta aggatcagaa ccttaagtgg acgaatttcc tgaccagacg
     7441 cacgagaagt tgtcatcaca aagacgtttt cttcacgcaa gaaattgacg gcgggtagct
     7501 cgtccggcac acgaataggc ataacctgat tacctcacta catacgctta tacgtttaga
     7561 catccagata gctgaagata accagaaaat ttgccaatgt cgagcctgca tgttgaaggt
     7621 gagaaagttt caaggaaaag gctacgttag aatataagaa tgacgaaaag gagagaaaat
     7681 ggttattagt attcgccgct cacgacatga ggaaggggag ggactcgttg cgatttggtg
     7741 tcgatctgtc gatgccactc acgatttttt atcagcagag tatcgggccg agctggagga
     7801 gctggttcgt tcctttctgc cggaagcacc gttgtgggtc gcggttaatg agcgggaaca
     7861 gccggttggg tttatgttgt taagtgggca gcatatggat gcgctgttta tcgatcctga
     7921 tgtgcgcggc tgcggcgtgg gtcggatgct ggtggagcat gcgctctcga tggcaccgga
     7981 actgacaacc aacgttaatg agcaaaatga gcaggcggtt gggttctata agaaggtggg
     8041 ttttaaggtt acgggacgct ctgaggtgga cgatttgggg aaaccgtatc cattgctgaa
     8101 tctggcatat gtcggggcgt aggcgttttt ttctcatccc gctgggctga acttcaggtt
     8161 tcgtacccac caatggtgac acgattattt ctgcttaagc attgccatga ccgcactatc
     8221 actgagtacc actgattgcg catggatgtg gaactggcga tatttcatta acgttgcccc
     8281 agcgaccact tcatgaagaa tacgttcctc aacctgtgac aaaccgccct cattcatttc
     8341 cagcgcgcta acaacaatat ccatacgttt tgctggcaaa aaagaatgcc aggttgaacg
     8401 tgggtggagt tgcagaacaa gatcatgcag aactttttct gcaacaaaga aattggcgat
     8461 taacaggcgc tggttactga aggcagcgtc tcctgacact tcacgtttgc tttcaagatc
     8521 gcgaacagta attttattac gactaatttg gatatacaga actgacatga gattcccttc
     8581 atcatgcaaa taattgatat gcaaaaaacc ccggccttat ggaccggggt tttt
//
LOCUS       DAJOWB010000169         8580 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJOWB010000169 DAJOWB010000000
VERSION     DAJOWB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.169"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..546
                     /locus_tag="OHO85_004327"
     CDS             <1..546
                     /locus_tag="OHO85_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HCQ2673599.1"
                     /translation="FDDFMRQHRFSKVDFDTQQMIHFINRFNMNKGLIDKNNNFFIQL
                     IDQALRSTDDMIKANAWYLYKEWIRSDDVSPIFIETEEKLRTFNTNKLTRNDNIFILF
                     SSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEMLPVNLTQETLFSSKSQDK
                     HALFPIFTASWRAHRIMNKGV"
     gene            769..1266
                     /gene="ubiC"
                     /locus_tag="OHO85_004328"
     CDS             769..1266
                     /gene="ubiC"
                     /locus_tag="OHO85_004328"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCQ2673600.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1279..2151
                     /gene="ubiA"
                     /locus_tag="OHO85_004329"
     CDS             1279..2151
                     /gene="ubiA"
                     /locus_tag="OHO85_004329"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCQ2673601.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2306..4729)
                     /gene="plsB"
                     /locus_tag="OHO85_004330"
     CDS             complement(2306..4729)
                     /gene="plsB"
                     /locus_tag="OHO85_004330"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCQ2673602.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4900..5268
                     /gene="dgkA"
                     /locus_tag="OHO85_004331"
     CDS             4900..5268
                     /gene="dgkA"
                     /locus_tag="OHO85_004331"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCQ2673603.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5378..5986
                     /gene="lexA"
                     /locus_tag="OHO85_004332"
     CDS             5378..5986
                     /gene="lexA"
                     /locus_tag="OHO85_004332"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCQ2673604.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            6059..7384
                     /gene="dinF"
                     /locus_tag="OHO85_004333"
     CDS             6059..7384
                     /gene="dinF"
                     /locus_tag="OHO85_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCQ2673605.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQELADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7500..7709
                     /locus_tag="OHO85_004334"
     CDS             7500..7709
                     /locus_tag="OHO85_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCQ2673606.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7751..8266)
                     /gene="zur"
                     /locus_tag="OHO85_004335"
     CDS             complement(7751..8266)
                     /gene="zur"
                     /locus_tag="OHO85_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCQ2673607.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1984 a         2067 c         2314 g         2215 t
ORIGIN      
        1 ttcgatgatt ttatgcgtca acatcgattc agcaaggtgg atttcgatac ccaacagatg
       61 atccatttta ttaacaggtt taatatgaat aaagggctga ttgataaaaa taacaatttt
      121 tttatccaac ttatcgatca ggccttacga tcaacggatg atatgatcaa agcaaatgcc
      181 tggtatcttt ataaagagtg gattcgtagt gatgatgttt cacctatatt tatagaaact
      241 gaagaaaagt taagaacctt taacactaat aaattaacac gaaacgataa tatatttatc
      301 ctgttctcct ctgtcgatga tgggcctgtt atggtggtaa gctcccagcg cttacatgat
      361 atgttgaatc ctacaaaaga taccaattgg aattccacgt atatctacaa atccagacat
      421 gagatgttgc ctgttaatct tactcaggaa acacttttca gctccaaatc tcaggataaa
      481 catgcgcttt tccccatttt tactgcgagt tggcgagctc atcgtataat gaataagggt
      541 gtttaagtaa aggaaaacat caccgttcct ggcatcctgg gcggtgatgc ccctatggtt
      601 gccctctcca gcgcgggcat cggcaaagcg taaggttcaa cagcgtttta ccacttcatg
      661 cgattgttgc gtttttgttg cgtattagat cacttaattt gctttacatc tcccgtaaac
      721 acttttctgc gatacaatgc ctttacgtta tgtaacggag agttcggcat gtcacacccc
      781 gcgttaacgc aactgcgtgc gctgcgctat tttaaagaga tccctgccct ggaaccgcaa
      841 ctgctcgact ggctgttgct ggaggattcc atgacaaaac gttttgaaca gcagggaaaa
      901 acggtaagcg tgacgatgat ccgcgaaggg tttgtcgagc agaatgaaat ccccgaagaa
      961 ctgccgctgc tgccgaaaga gtctcgttac tggttacgtg aaattttgtt atgtgccgat
     1021 ggtgaaccgt ggcttgccgg tcgtaccgtc gttcctgtgt caacgttaag cgggccggag
     1081 ctggcgttac aaaaattggg taaaacgccg ttaggacgct atctgttcac atcatcgaca
     1141 ttaacccggg actttattga gataggccgt gatgccggac tgtgggggcg acgttcccgc
     1201 ctgcgattaa gcggtaaacc gctgttgcta acagaactgt ttttaccggc gtcaccgttg
     1261 tactaagagg aaaaaaatat ggagtggagt ctgacgcaga ataagctgct ggcgtttcat
     1321 cgcttaatgc gtacggataa gccaattggc gcgttactgc tgctctggcc aacattatgg
     1381 gcgttgtggg tggcgacacc gggcgttcct cagctctgga tcctggcggt atttgtcgcg
     1441 ggtgtctggc tgatgcgcgc tgctggatgt gtggtgaatg attatgctga ccgaaagttt
     1501 gatggtcatg ttaagcgcac ggcgaaccga ccacttccca gcggcgcggt aacagagaaa
     1561 gaggcgcgcg cgctgtttgt agtgctggta ctgatttcgt ttttactggt actgacgctg
     1621 aatacgatga ccattctgtt gtcgattgcc gctctggcgt tggcgtgggt gtacccgttt
     1681 atgaagcggt atacccatct cccgcaagtg gtgctgggcg cggcgtttgg ctggtcgatt
     1741 ccaatggctt ttgccgctgt gagtgagtcg gtgccattga gttgctggtt aatgttcctc
     1801 gccaatattc tctgggcggt ggcttacgac acgcagtatg cgatggttga ccgcgatgat
     1861 gatgtgaaga ttggcattaa atccacggca atcctgttcg gccaatacga taaattgatt
     1921 attggtattt tgcagattgg cgtactggca ctgatggcga tcatcggtga gttaaatggc
     1981 ttaggctggg gatattactg gtcaattgtg gtggctggcg cgctgtttgt ttatcaacaa
     2041 aaactgattg ccaaccgcga gcgtgaagcc tgctttaaag catttatgaa taataactat
     2101 gttggtctgg tactgttttt aggactggca atgagttact ggcatttctg atgatgtaaa
     2161 aaagccggat gatcatccgg ctttcttctg ggttgcctta tgcgcggcgc ttctcaggcc
     2221 tacacaacac atcgcaattt attgaatttg cagattatgg aaggccggat aaggcgtttt
     2281 cgccgcatcc ggcaattctc tctgattacc cttcgccctg cgtcgcactc tcaatcgtca
     2341 aacgcacgtc tgatgtaatc aactccgcca gcaactgata aaccttcatc gtttctgccg
     2401 gttcggcgtc gccgctatcg ctgatatacc cttcatcacg cagcgtcagc accagagaac
     2461 tgaacaccgc cttgtcgaag aactccggcg cgttgatgcc gtgcagcacg gagagacgtt
     2521 gcgcgacggt gcggctctct ttctccagcg taccgcggtt gatcgacggg ttggcactca
     2581 acaaccagaa ggtgatggca taacgttgca gcgtttcgcg cgcgcctgcg gccagcagct
     2641 gtagggtgcg agaatgcgcc gggttgatat gcaactcatc atcttgcagg gtaatcagcc
     2701 cctgacgttg catctcattt gccagcgcat caataacgtc cggcaactcg tcgcgatccc
     2761 agcgcaggaa cagctccgct ttcagcattg ggtaaagcac attgacgtgc tccatcaata
     2821 cgtcgcggga gatgtggcga tgctgggtga caattgccgc catcagcgaa ggcagcacca
     2881 acatatgcgc aatgttgttg cgatagtagg tcatcagcac cgcttgctcg cgtggcagaa
     2941 tgatgatgtc gccgatagtg tctttctcga cttcaaactt gttcatttgc agcgcgtgat
     3001 cgataagctc gctggcgctg gctgaaggaa cggtagagtc cgtggagtaa ggtacgttgc
     3061 gcatcagatc caggtagcag ttgagttgct cggttaactg ctcgcgggtg agtgagcgct
     3121 gacgtgatgc cagaagcgca gtacaacaca ggttcatggc gtttgccgcg cctgcgttgt
     3181 taatgcgtac catcagatcg gcagcaatat tattgaccgt cggcgttaac caggccggac
     3241 gcaccgcttc gatgggatcg atagattcac gccagtctgg tacgtgctgg ttaaggtagg
     3301 tcatcaacgg cattggttca ccgaagttga cgtaaccctg accgagatta cgcagcttgc
     3361 ttaaaccgcg cagcatctgc ggcaggctct ctttctcttt cgtcgcgccg cgcagttctt
     3421 tggcgtaagt acccacttcc atgacgtgct cataaccgat atagatcgga atcagcgtaa
     3481 tcggacgcgt gccgccacgc agcatcgcct gaatggtcat cgacagcgta ccggttttcg
     3541 gatccagcaa acgccccgtg cgggaacgac cgccttccac gaagtactcg acggaataac
     3601 cacggctgaa cagttcgccg agatactcac ggaaaacggt ggaataaagt ttattgcctt
     3661 taaaagtacg gcgaataaag aacgccccca gacggcggaa aatcggcccg gccggccaga
     3721 agttcaggtt gatcccggcg gcgatatgcg gcggcaccag cccctggtgg tacagcacgt
     3781 aagaaagtag caggtagtcc atgtgactgc ggtggcaagg cacatacacc agctcatgac
     3841 cgtcgtgggc cagctggcga acgcgctcgg cgttatggac gttgatgccc tgataaagtc
     3901 ggttccaggt gaagcccaga atgcggtcag tcaggcggat catctcgtaa gagaaattcg
     3961 ccgcaatctc ttccatcagc gcaatcgcgt tctgctgcgc tttttcatgg gagatttttt
     4021 tgctgcgcgc ttcatcttct accgctttgg caatggcgcg ggaggcgagc agcttattaa
     4081 acagatcctg acgagcaggc agacgtgggc ccacagcagc cagacgttga cgggcaaagt
     4141 gcatacgcgc cacgcgcgcc agtttctgtg cgatagtttt atccgtgccg tgttcatccg
     4201 ccatacggcg cagcgaaact gacggcgaga aacgcacaaa actgtcgcga ccgagccaca
     4261 gtacggcgaa aaatttctgt acgccgttaa gcatacgcag cggcgggttt acttcgcctt
     4321 tttcacgccc cggcgcgcga ccaaacatca ccgatactgg caccatctgc acatccagat
     4381 ttgggttgct gcggtgcaaa tcaagatagt cgtggaacag cttaatagac tcttctttcg
     4441 gcgtgtaata ggtgaacaca cgcggcccgc cgtgaatgaa cacatagcgc ggcagtagcg
     4501 tgccgtcgat ttccagcggc tctaacgggt caggcaagtc atgagccaga cactgggcgc
     4561 gcaacgtcag caaatctgct ttcgagttgt acggtaaaac gtacataatt ggacgagagg
     4621 tatccagccc cagttccggg gcaggatctg ccggaataga cttgcttttt accaggatgc
     4681 ttaatggtaa attcagtaat ttgtagtaaa ttcgtggcca gccggacata aacgatgtaa
     4741 agcctctggt taataatgca aatgcgcggc aaggatagca gaaagtcatg ggaaattctg
     4801 tggtatccgc tcatgtttcg cgcggcgtta cgcaaacccg aatcatcgga tttaacggta
     4861 cactgatatt gacgctcata atgtaaaaag gttctttcaa tggccaataa taccactgga
     4921 ttcacccgaa ttatcaaagc tgctggctat tcctggaaag gtttacgcgc tgcatggatc
     4981 aacgaagcgg cattccgtca ggaaggcgta gcggtattgt tggcggtggt catcgcctgc
     5041 tggctggatg tggacgcaat tacccgcgtg ctgcttatca gctccgtgat gctggtgatg
     5101 attgtggaaa tcctcaatag cgccatcgaa gcagtggttg accgaattgg ctctgaatac
     5161 catgagcttt ccggacgcgc aaaagatatg ggatccgccg cggtgctgat tgccattatc
     5221 gtcgccgtga ttacctggtg catcctgtta tggtcgcatt ttggataacc cttccagatt
     5281 tcgataaatc tctggtttat tgtgcagttt atggttccaa aatcgccttt tgctgtatat
     5341 actcacagca taactgtata tacacccagg gggcggaatg aaagcgttaa cggccaggca
     5401 acaagaggtg tttgatctca tccgtgatca catcagccag acaggtatgc cgccgacgcg
     5461 tgcggaaatc gcgcagcgtt tggggttccg ttccccaaac gcggctgaag aacatctgaa
     5521 ggcgctggca cgcaaaggcg ttattgaaat tgtttccggc gcatcacgcg ggattcgtct
     5581 gttgcaggaa gaggaagaag ggttgccgct ggtaggtcgt gtggctgccg gtgaaccgct
     5641 tctggcgcaa cagcatattg aaggtcatta tcaggtcgat ccttccttgt tcaagccgaa
     5701 tgctgatttc ctgctgcgcg tcagcgggat gtcgatgaaa gatatcggca ttatggatgg
     5761 tgacttgctg gcagtgcata aaactcagga tgtacgtaac ggtcaggtcg ttgtcgcacg
     5821 tattgatgac gaagttaccg ttaagcgcct gaaaaaacag ggcaataaag tcgaactgtt
     5881 gccagaaaat agcgagttta aaccaattgt cgttgacctt cgtcagcaga gcttcaccat
     5941 tgaagggctg gcggttgggg ttattcgcaa cggcgactgg ctgtaacata tctctgagac
     6001 cgcgatgccg ccaggcgtcg cggtttgttt ttcatctctc ttcatcaggc ttgtctgcat
     6061 ggcattcctc acttcatctg ataaagcact ctggcatctc gccttaccca tgattttctc
     6121 caatatcacc gttccgttgc tgggactggt cgatacggcg gtaattggtc atcttgatag
     6181 cccggtttat ttgggcggcg tggcggttgg tgcaacggcg accagctttc tctttatgct
     6241 gttgctgttt ttacgcatga gcaccaccgg gctgactgcg caggcttatg gtgccaaaaa
     6301 tcctcaggca ttagcccgtg cgctggtgca accgttgctg ttggcgttgg gggctggggc
     6361 gttaattgcg ctgctgcgta caccgattat cgatctggcg ctgcatattg ttggcggtag
     6421 tgaagcggtc ctggaacagg cgcggcgctt tcttgaaatc cgctggttaa gcgctccggc
     6481 gtcgctggcg aatctggtat tactcggttg gttactcggc gtgcaatatg cccgtgcgcc
     6541 agtaattttg ttagtggtcg gcaatatcct caacattgtg ctggatgtct ggctggtgat
     6601 ggggctgcat atgaacgtgc agggcgcggc gctggcgacg gttattgcgg aatatgcaac
     6661 attgctgatt ggtctgctaa tggtgcgtaa aatcctcaaa ctacgcggaa tttccggcga
     6721 aatgctgaaa actgcctggc gaggaaactt ccgtcgcttg ctggcgctta accgcgatat
     6781 catgctgcgt tcgctgttgt tgcaactctg tttcggcgcg atcaccgtac ttggtgcgcg
     6841 actggggagt gacattatcg ctgttaacgc ggttctgatg acgctactca cctttaccgc
     6901 ctatgcgctg gatggttttg cctacgcggt tgaagcacat tccggtcagg catacggtgc
     6961 gcgcgacggt agccagttgc tggatgtctg gcgggcagcg tgccgccagt cggggatcgt
     7021 agcgctactg ttttcggtgg tttatttgct ggcaggggaa cacatcattg cgttactgac
     7081 gtcgttaacc cagattcagg agctggccga ccgctatctt atctggcagg tgattttgcc
     7141 gttggttggc gtctggtgtt atctgctgga cggcatgttt ataggcgcaa cgcgtgccgc
     7201 cgaaatgcgt aacagtatgg cggtggccgc cgcaggtttt gcgctgacgc tccttacgct
     7261 gccgtggctg ggtaatcatg gtttgtggct ggcattaacc gtctttctgg cgttgcgcgg
     7321 gctttctctg gcggctatct ggcggcgtca ctggcgcaac ggtacctggt ttgccgcaac
     7381 gtgacggtta aaaattctga ataaataatc ctaagccaaa ttgctgacta cacttaatct
     7441 cacgttcaga agaaaagtga acgtactctc attcacaacc taacgatgag gtcttgatta
     7501 tgaataaaga tgaagccggc ggtaactgga aacagtttaa aggtaaagtg aaagagcaat
     7561 ggggcaaact gaccgatgat gatatgacga tcattgaagg taaacgtgat caactggtcg
     7621 gtaaaatcca ggaacgttat ggttatcaga aagatcaggc agaaaaagag gtcgtggatt
     7681 gggaaacccg caatgaatat cgctggtaat taatccctcc tgcccgacgt gtacaaggat
     7741 gtacgccctc ttaacgcggt ttctttttca cctgcacaga gtgatcatgc tggcactgtt
     7801 caggatgacg acacgcttcc acttccacgc atgccgcaca gagcccatgt gcttcaatca
     7861 cattatgccg cagggcaaac cccatttttg ccgccagcgt atgcataatg tcttccacgc
     7921 cttctgcaca ctcttctttc accgcgccgc agcgatcgca aataaacatg gctgacgtat
     7981 gcgtgggctg gtcgaacaga tgacagagca cataactgtt ggtggattcc accttatgca
     8041 caaaaccttg ctcaagcaga aaatccagcg cgcgataaac cgttggcggc ttggcttgcg
     8101 gttcagcttc gcgcagcaaa tcaagcagat cataagcgct gatagcgcca tcctgcagac
     8161 tcatcaggcg caacacttcc aggcgctgtg gggtcaggcg cacattacgc tgcgcgcaga
     8221 ttttttcagc ctgcgctaat aactcctgcg ttgtggtctt ttccatcggg cacctcaaag
     8281 tgggggttaa gaatgtcctt actttaccat gttccaggaa aaacaccgag aacccttatt
     8341 gttgccgtaa tgttgatttt ctgttttgta cgtaaggtgt tgtgttgctt ggtcgtacca
     8401 ttatcaacac gataataatt aataattcat ttttaaataa attgcttcaa gttaactcgc
     8461 tatttattta actgggtttg atcgcatacc tgtagttcag aaattatgag cgatttttga
     8521 tagtgaccag atggcaaaac acttataagg aggacacggt acgacaaaaa aggtagatga
//
LOCUS       DAJOWB010000170         8540 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJOWB010000170 DAJOWB010000000
VERSION     DAJOWB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.170"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(195..271)
                     /locus_tag="OHO85_004336"
     tRNA            complement(195..271)
                     /locus_tag="OHO85_004336"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(235..237),aa:Asp,seq:gtc)
     gene            complement(404..1135)
                     /gene="dnaQ"
                     /locus_tag="OHO85_004337"
     CDS             complement(404..1135)
                     /gene="dnaQ"
                     /locus_tag="OHO85_004337"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCQ2673608.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DDELAAHEARLDLVQKKGGSCLWRA"
     gene            1200..1667
                     /gene="rnhA"
                     /locus_tag="OHO85_004338"
     CDS             1200..1667
                     /gene="rnhA"
                     /locus_tag="OHO85_004338"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCQ2673609.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1664..2386)
                     /locus_tag="OHO85_004339"
     CDS             complement(1664..2386)
                     /locus_tag="OHO85_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCQ2673610.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2420..3175
                     /gene="gloB"
                     /locus_tag="OHO85_004340"
     CDS             2420..3175
                     /gene="gloB"
                     /locus_tag="OHO85_004340"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCQ2673611.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3247..4605
                     /gene="mltD"
                     /locus_tag="OHO85_004341"
     CDS             3247..4605
                     /gene="mltD"
                     /locus_tag="OHO85_004341"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCQ2673612.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4653..5423)
                     /locus_tag="OHO85_004342"
     CDS             complement(4653..5423)
                     /locus_tag="OHO85_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCQ2673613.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDISHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGFFTSDIIMVDAHKAA"
     gene            complement(5501..6301)
                     /locus_tag="OHO85_004343"
     CDS             complement(5501..6301)
                     /locus_tag="OHO85_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCQ2673614.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6542..7456
                     /locus_tag="OHO85_004344"
     CDS             6542..7456
                     /locus_tag="OHO85_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2673615.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMASAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7453..8256)
                     /gene="dkgB"
                     /locus_tag="OHO85_004345"
     CDS             complement(7453..8256)
                     /gene="dkgB"
                     /locus_tag="OHO85_004345"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCQ2673616.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVITALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8419..8495)
                     /locus_tag="OHO85_004346"
     tRNA            complement(8419..8495)
                     /locus_tag="OHO85_004346"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8459..8461),aa:Asp,seq:gtc)
BASE COUNT         2284 a         2051 c         2073 g         2132 t
ORIGIN      
        1 tattctctgg aaatatattt aagctgaatg gttcgatatc attattgaat tttaatgaat
       61 gagacaacaa gcgagaatca cgcacttacg ccagagtaaa atcattgaac agagaaatgg
      121 tggaattcaa atacaaaaaa accgcaaaat taaaaatctt gcggctctct gaactcattt
      181 tcatgagtga atagtggcgg aacggacggg actcgaaccc gcgaccccct gcgtgacagg
      241 caggtattct aaccgactga actaccgctc cgcgttgtgt tccgttggga acgaggcgaa
      301 tattacggat tgcctcgacc ttcgtcaacg gtttttctca tcttttgaat cgtttgctgc
      361 aaaaatcgcc caagtcgcta tttttagcgc cttttacagg tatttatgcc cgccagaggc
      421 agcttccgcc cttcttctgc accagatcaa gacgggcttc atgtgctgca agctcgtcat
      481 ctgtcgcaaa aacaacgcgt aacttacttg cctgacgtac aatgcgctga attgttgctt
      541 caccttgttg ctgttgtgtc tctccttcca tcgcaaaagc catcgacgtt tgaccaccgg
      601 tcatcgccag ataaacttcc gcaaggatct gggcatcgag taatgccccg tgtagcgttc
      661 gtttactgtt atctatttcg tagcgggcac ataacgcatc gaggctgttg cgcttaccag
      721 gaaacatttt cctcgccacc gcaaggctat cggtgacctt acagaaggta ttggtcttcg
      781 gaatatcgcg cttaagcaac gaaaactcgt agtccataaa gccgatatcg aacgctgcgt
      841 tatggatcac caactccgcg ccgcggatat agtccatgaa ctcatcggct acttcggcaa
      901 acgtgggctt atcgagcaaa aattcatcgg caataccatg tacgccaaag gcttcaggat
      961 ccaccagccg atcgggtttg agataaacat ggaagttatt gcccgtcagg cgacggttca
     1021 ccacttcaac ggcaccaatc tcaatgatct tgtggccttc atagtgcgca ccaatctggt
     1081 tcataccggt ggtttcggta tcgagaacga tctggcgtgt aattgcagtg ctcatagcgg
     1141 tcatttatgt cagacttgtc gttttacagt tcgattcaat tacaggaagt ctaccagaga
     1201 tgcttaaaca ggtagaaatt ttcaccgatg gttcgtgtct gggcaatccc ggacctgggg
     1261 gttatggcgc tattttacgc tatcgcggac gcgagaaaac ctttagcgct ggctacaccc
     1321 gcaccaccaa caaccgtatg gagttgatgg ccgctattgt cgcgctggag gcgttaaaag
     1381 aacattgcga agtcattttg agtaccgaca gccagtatgt ccgccagggt atcacccagt
     1441 ggatccataa ctggaaaaaa cgtggctgga aaaccgcaga caaaaaacca gtaaaaaatg
     1501 tcgatctctg gcaacgtctt gatgctgcat tggggcagca tcaaatcaaa tgggaatggg
     1561 ttaaaggcca tgccggacac ccggaaaacg aacgctgtga tgaactggct cgtgccgcgg
     1621 cgatgaatcc cacactggaa gatacaggct accaagttga agtttaagcc tgtggtttac
     1681 gacattgccg ggtggctcca accgcctggc gaattcgtgg cttgttttta ctctgtttca
     1741 tcggatttag cgttaaagga atagtccgtt tccgggcaac aataagttgt aagcaaccaa
     1801 gcgcaggaat atgcgcattc aatagttttc ctccgtgttt gttccacggg agaacgtgga
     1861 aacggctggc gtgtagcact tcaaaattca acaaagagag ccagtccagc tgccgcatca
     1921 gagtaaacat ccggctgtta tagggcgagg ttttgcgcaa taccggcaca agtttgcgta
     1981 atcccattaa actgatggga ttgaagccac taatgaccag ccagccatca tcaatcaata
     2041 cccgatcggc ttcacgcaat aaacgatgcg gatcggtgca ccacggcaat gtatgtgcca
     2101 gtagacaaac atcaacggat ttatcggcaa aaggaagatg aagtgggtcc gcctgtacct
     2161 ggacgggcat tccttgcgca gaaacattca cttgatgaga aaccgcgcac gcttcgcaat
     2221 tgatttctgc gcttaaattg ccaatcttaa gcagatgaaa accatacatt ttagtgaacc
     2281 acgggttgag ctggcgctcc agcgccttgc gataaagctc tccccagggc aaatcgcccc
     2341 agcaatcagg agccacgaca gtttgaggga ctcttgccgg tttcatcaca accttccgtt
     2401 tcacactgag aggtaatcta tgaatcttaa cagtattccc gcctttgatg acaattacat
     2461 ctgggttttg aatgatgaag caggtcgctg cctgattgtc gatcccggag acgcagagcc
     2521 agtattaaac gccattgccg ccaataactg gcaaccagag gccatatttc tcacccacca
     2581 tcaccacgat cacgttggcg gcgtaaaaga actggtggaa aagtttccgc aaattgtggt
     2641 gtatggtcca caagagacac aagataaggg aacaacacag gtagtcaaag atggcgaaac
     2701 tgccttcgtt ttagggcatg aatttagtgt aattgccaca ccgggtcaca ctttaggaca
     2761 tatctgttac ttcagcaaac cttatctatt ttgcggcgac acgctgtttt ctggtgggtg
     2821 tggtcggttg tttgaaggga caccatcaca aatgtatcaa tcacttaaaa agttaagtgc
     2881 gttacctgac gatacattgg tatgttgtgc tcatgaatat acgttatcaa atatgaagtt
     2941 tgctttgagt attcttccgc acgatttgtc cataaatgat tattatcgta aagttaagga
     3001 gttacgggca aaaaatcaaa taacactacc cgtaattctg aaaaatgagc ggcaaattaa
     3061 tgttttttta agaacagaag atattgattt aattaatgta attaatgaag aaacattatt
     3121 gcaacaacct gaagaacgtt ttgcatggtt aaggtcaaag aaagataggt tctgataaaa
     3181 ctttcttgtc atcggctccg ttcgccgtta tgatcggtcg tcttttaagc aactattgac
     3241 acacacatga aggcaaaagc gatattactc gcctctgtcc tgctcgtggg ctgccaaagt
     3301 accggcaacg ttcaacagca cgcacagagc ctttctgcag ctggtcaagg ggaagcagca
     3361 aagtttacaa gtcaggcacg atggatggac gatgggacgt ctatcgcgcc agatggtgac
     3421 ttgtgggctt tcattggcga cgagctaaag atgggaattc cggaaaatga ccggattcgc
     3481 gaacagaaac agaaatattt acgcaataag agctatctcc acgatgtaac tttacgggca
     3541 gagccgtata tgtactggat agccgggcaa gttaaaaaac gtaacatgcc tatggaactg
     3601 gtactactac ccatagtgga gagcgctttt gatcctcacg caacgtctgg cgccaatgcc
     3661 gcaggcatct ggcagatcat tccgagcacg gggcgcaatt atggtttgaa acagacccgc
     3721 aattatgacg cgcgtcgcga tgttgttgct tcaacaactg ccgcgctgaa catgatgcag
     3781 cgtctgaaca agatgtttga cggcgactgg cttctgaccg tagcggctta taacagcggc
     3841 gaaggtcggg tcatgaaggc aattaaaacg aacaaagcgc gtgggaaatc cacggacttc
     3901 tggtcgttac cgttgccgca ggaaacgaag cagtacgtgc ctaaaatgct ggcattgagt
     3961 gatattctca aaaacagcaa gcgttatggc gtacgtctgc caacgaccga tgaaagccgt
     4021 gctctggcgc gtgtgcacct gagcagcccg gttgaaatgg cgaaggttgc agatatggcg
     4081 gggatttccg tcagcaagct gaagacattc aacgctggcg taaaaggctc cacgctgggc
     4141 gcaagtggtc cgcagtacgt gatggtgcca aagaagcatg cagatcaact gcgtgaatct
     4201 ctggcttcag gcgaaattgc tgctgtacag tcgacgctgg ttgccgacaa tacgccgctt
     4261 aacagccgtg tttacaccgt acgctctggc gacacgcttt caagtatcgc ttcacgtctc
     4321 ggcgtaagca ccaaagattt gcagcagtgg aacaaactgc gcggatctaa gctgaagcca
     4381 ggccaaagtt tgacgattgg tgcaggcagt agcgcacagc gactggcaaa caacagcgat
     4441 agcattacgt atcgtgtgcg caaaggcgat tcgctttcaa gcattgctaa acgccacggc
     4501 gtgaacatca aagatgtgat gcgctggaac agcgacactg cgaatctgca accaggcgat
     4561 aagctgacgt tgtttgtgaa aaacaacaac atgccagatt cctgacaaac cagataataa
     4621 aaaggcaccg attcccccgg tgcctttttt atttatgccg ctttatgtgc atctaccatg
     4681 atgatatcac tggtgaaaaa gccatcattc tgcaaggcaa aatacgttct cacctctgtc
     4741 gatgcgctct gttggtaaat gcgaatagcg tctactaacg cttctggcgt acgcattctc
     4801 gcgacccatg aagaaaattc cagcggtaac ttatctgtaa ttaaattatc aactatcaga
     4861 ttggcttcat tgattaacgt caaccactca ccgctggcgt agtttcgtac gtgagagata
     4921 tcgcgtaatg cttctaccgt ctgtaaccag atgtcgcgca ctgggtgacc cggagacatt
     4981 acgtccatca caatcagcct accgccaggt ttcaatatcc tattcacttc tcgcagtgct
     5041 gcaccaacat catgccagtg atgggcagaa taacggctga taacaatatc aaatgcgtta
     5101 tcggcaaatg gcagactttc ggcatatccc tggcgggtgg tgatattttt cagttgccgg
     5161 gcttcggcag cttgtgccac gacatccagc atatgggcag ataagtcata cgccaccacc
     5221 gtgctcacgt tttgcgcagc gacaaagctg gcatgccctg ctccgcagcc catatcaagt
     5281 acacttgcac caggataatc agccagacgc accgccaggc gctgtaaatc tcgtccggat
     5341 gcatgtacgg tactggtcag atattcgcag gcttgagagc taaactgctt ttctacgtgg
     5401 tcatggtggg attgtgttgt cattgcgctg tccttttgtt gtttgagtat aaaaggacag
     5461 cgccaaacgt tggtctgccc tgtggcagac ctgacatacc ttatttatca ggcttgccgg
     5521 gactgaattc aacgagtagc ggattgtgat cggaagcgcg cgtaaccaat acagaagctt
     5581 cgctgacgtt cagaccacgg tagaaaacaa aatcgagcgg gcggccaaac gcccgacggc
     5641 gctgatcatc ggtaaaacgc acctggcgca gcgacatttc ccgcgcaaag cgatataacg
     5701 cgttcatcct tctacggctc caggcattga aatctcccgc cataatgacc gggccgctgt
     5761 ggtgagctat ctgatcacca ataggaagta actgcttact atagacatcc acgcccagac
     5821 tgaagttgac ggcgtgtata ttaaccacca tcaacaggcg ggtgtcaggt aatggataga
     5881 ccgtcaccag tgccgacttc gccagacgca aaatgggttc tcgttcacgt aacgggcagc
     5941 aatacactgg atgcgccgcc gaaagggtca ttacaccgga aggatgttgt ggcagcacga
     6001 aagcgggcac ctgatcggcg gcaagatagt tagcggtcgc aaactgtact aactctggcg
     6061 ttgtctgcgc ttcctgcaat aacaccagat gtgcatcttt gccgtagttc tttaataccg
     6121 acaaccattc agcgcgttgc tgtttgtata tgttccacac caggatccga atacgctctt
     6181 cggtacttaa cggttctcca ggtggtaatg cctggccgat gctcgcaaaa gaccccggcg
     6241 gtaagatcct ttccgcaggt tgtccggcaa catagcgcat ggcataggtg ttttttcgca
     6301 cttttgactt tgaaacctct attacgtcaa cccttccgca ctcgggaggg ttctactgtt
     6361 atagggattt tagctcactc tttcaacgag caattgtata ttgttatgta agcaagtgct
     6421 tacaccggtc agaattttgc tcattatgga gaacattaaa tcgcatgaca tatcacgcat
     6481 ccagatggtt taatgacaaa taaattcaaa atatctcttc tattttgcaa caggagcaaa
     6541 aatgaaagcc acgtcggaag aactcgccat ttttgtttcg gtcgtcgaaa gcggcagctt
     6601 tagccgggca gcggaacaat tagggcaagc aaactcagcg gtaagccggg cggtgaaaaa
     6661 gctggagatg aaacttggcg ttagcctgct taatcggacc acgcgacaac ttagcctgac
     6721 ggaagaaggc gagcgttatt ttcgtcgcgt acagtcaatt ttgcaggaga tggcatcggc
     6781 agaatcagaa attatggaga cgcgtaatac accgcgtgga ctgttacgga tcgatgccgc
     6841 aactccagtg gtgctgcact ttctgatgcc gttaattaag cctttccgtg aacgctatcc
     6901 ggaagtcact ttgtcgctag tctcctccga aacgattatt aatttgatcg aaagaaaagt
     6961 ggatgtcgcg atacgcgctg gtacgttaac ggattccagc ttacgtgcca ggccgttatt
     7021 taacagttat cgaaaaatta tcgcctcccc cgattatatt tcccgctacg ggaagccaga
     7081 aacgatcgac gatttaaagc aacatatttg cctgggattc actgaacccg cttccctcaa
     7141 tacctggccg atagcccgta gcgatggaca attacatgag gtgaagtacg gtttgtcatc
     7201 caatagtggg gaaacactga aacagctttg cctgagtggc aacgggattg cgtgtttgtc
     7261 cgactacatg atcgacaaag aaatcgctcg cggagaattg gtggagttaa tggcagataa
     7321 agtgttgcca gtggaaatgc cattcagtgc cgtctattac agcgaccgtg cggtaagtac
     7381 gcgcatccgg gcttttatcg atttccttag cgagcatgta aaaacagctc ccggaggagc
     7441 tgtcagagag gcttaatccc attcaggagc cagaccttcc gggctaacca ggcggtcgtt
     7501 gcaatccagt gcggcgatcg cttttttatc ttcggcatca agctgtaaat tttgtgcctt
     7561 aagattactt tccaggtttt tacgtttagt agaagaagga attactgagt aaccttcccc
     7621 catagcccac gccagaatca cttgtgccgg agtcgcattg tgtttagctg cgatacgagc
     7681 aataacctca tctttcaggg ccttaccata cgccagcgtc atataggaag taatatggat
     7741 gccgtgctgt ttagcccagg caaccacttt acggttttgc agataaggag agagttcaat
     7801 ctggttagta gcgatgtttt cagcaccaac agcagcaatc gctttttcca tcaacgggat
     7861 cgtgaagttg gaaataccga tctcacgcgt cagcccttgt tttttggctt ccagcagcgc
     7921 ctgcataaac tcttcaacag agacttcatc gtttggtgac ggccagtgga ttagcgtcag
     7981 atcaacataa tcggtacgca atttttgcag gctctctttc agacttggga tcaatttgtc
     8041 tttgctgaga ttttcaatcc agattttagt ggtgatgtag agttcatgac gtggcacgcc
     8101 actttctgca atcgcctgac ctactgcggc ttcgttatca tagatttgtg cggtatcaat
     8161 tgcgcgataa ccaagttcaa gcgccgttat cacagatgaa ataacaacgt cgtctttcag
     8221 acggaaagta cctaaaccaa atgcagggat agccataata tgcctctttt aatgctattt
     8281 tcgttaactg aggtgattat gggcttcaga ctgttgagga aaaagagcgt aaaatgcaga
     8341 ggatttttgc gattctggca ataatagata tacagacgaa aaaaaacctc gagcgttaac
     8401 tcgaggcttc ttaataagtg gcggaacgga cgggactcga acccgcgacc ccctgcgtga
     8461 caggcaggta ttctaaccga ctgaactacc gctccaccga attcttttac aaccaccggt
     8521 tttatgaccg gcttactgct
//
LOCUS       DAJOWB010000171         8498 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJOWB010000171 DAJOWB010000000
VERSION     DAJOWB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.171"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(71..832)
                     /gene="kduD"
                     /locus_tag="OHO85_004347"
     CDS             complement(71..832)
                     /gene="kduD"
                     /locus_tag="OHO85_004347"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCQ2673617.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(862..1698)
                     /gene="kduI"
                     /locus_tag="OHO85_004348"
     CDS             complement(862..1698)
                     /gene="kduI"
                     /locus_tag="OHO85_004348"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCQ2673618.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTGTPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1985..3166)
                     /locus_tag="OHO85_004349"
     CDS             complement(1985..3166)
                     /locus_tag="OHO85_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCQ2673619.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3421..4650
                     /locus_tag="OHO85_004350"
     CDS             3421..4650
                     /locus_tag="OHO85_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCQ2673620.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            5110..5742
                     /locus_tag="OHO85_004351"
     CDS             5110..5742
                     /locus_tag="OHO85_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2673621.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNTYQIAEVLDMDIRSIYAYKQRIEKRMGDKINELFIRSHSVQH"
     gene            6076..6885
                     /locus_tag="OHO85_004352"
     CDS             6076..6885
                     /locus_tag="OHO85_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCQ2673622.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTDDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
     gene            6878..7360
                     /locus_tag="OHO85_004353"
     CDS             6878..7360
                     /locus_tag="OHO85_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673623.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(7393..7473)
                     /locus_tag="OHO85_004354"
     CDS             complement(7393..7473)
                     /locus_tag="OHO85_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673624.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(7509..7934)
                     /locus_tag="OHO85_004355"
     CDS             complement(7509..7934)
                     /locus_tag="OHO85_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673625.1"
                     /translation="MDIEFSQIHEMVYMHDIVNSDSKKKPRIPLKKFLNAENVLTQTT
                     SWTLKSRYVNVNSVNKVNVKSKVKNSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
     gene            8128..>8498
                     /locus_tag="OHO85_004356"
     CDS             8128..>8498
                     /locus_tag="OHO85_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673626.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKSVNPVKITMGSGLNY
                     IEQESLGGKYLTHDLSIKIADISEEIIQQAILSAMSIYKFSITDDLMSMAVNELIKLT
                     KIENNVDLNKFTTICTDVLSPR"
BASE COUNT         2552 a         1863 c         1685 g         2398 t
ORIGIN      
        1 tggattatga tgatctacag atataaaaaa ccctgccatg cggcagggtc ataaaagtaa
       61 gaagaatgaa ttaacgcgcc agccaaccgc catccacggc aatggtataa ccattcacat
      121 aatctgaagc gctggaggca aggaacacta ccggccccat caggtcactc ggcagtcccc
      181 aacggccagc tggaatgcgg tcgaggattt ccgcgctacg ttgttcatct gcccgtagtt
      241 gttgagtatt gttggtcgcc atgtaacccg gggctatcgc attaacatta atgttgtgtt
      301 tagcccattc gttcgccatc aatcgcgtca cacccatcac gccgcttttt gatgcggtat
      361 aagaaggcac acggatcccg ccctggaagg agagcattga cgcgatattg ataatcttgc
      421 cgccattgcc ttgcgcgata aagtgtttcg ccgctgcctg agacatgaag aatacgctct
      481 tgatattcag gttcatgaca tcgtcccagt ctttttcgct gaactcgaga gcgtcttcgc
      541 ggcgaatcaa tccggcgtta ttcaccagga tatcaatatg accaaattca gctaccgcgc
      601 gatccagcag tccaggaatg ccatcaatct ttcgcagatc ggcggtcagg cttaaaaaac
      661 gacgccccag cgccgtgacc tgctcgatgg tttcagtcgg ttcaacgatg ttaatgccaa
      721 caatgtcaca gcccgcttgc gccagcccca acgccatccc ctggcccagc ccagtatcac
      781 aaccagtgac gaccgcaact ttaccttcga gagaaaatgc acttaaaatc ataacaatac
      841 cttattcgtt tatgcccaca actagcgcaa atctttaacg gccacatggt ccatatcatc
      901 aaagacctgg ttttcaccaa ccatgcccca gataaaggta taagctttgg ttccgacgcc
      961 ggaatggatc gaccagctcg gggagatcac cgcctgctcg ttatgcatca caatgtgacg
     1021 cgtttcttgc ggttgcccca tcatgtggaa aacgcaggcg tcatcatcca tattgaaata
     1081 gaaataaact tccatccggc gctcgtgggt gtgacacggc atggtgttcc acaagttacc
     1141 tggagccagc tccgtcagcc ccatactcaa ttggcaggtt tccagcacat ccgggacaaa
     1201 atatttgtta atagtgcgac ggttactggt gaggttatcg cctaacgtga ctggagatac
     1261 ttcgtccggg gtgacttttt tggtgggata cgtcgtatgc gcgggtgcgc aattgtaata
     1321 aaacttcgcc ggagtgccgg tatcaatact ggcaaagaca acttcttttg cacctttacc
     1381 aacatacagg gcgtcgcggt gaccgatttc atagcattgg ccatcgacag taatcgtacc
     1441 ggcaccgcca atattgataa cacctaactc gcgacgttca aggaaatagc ttacgccgag
     1501 ttgtttacca acttccccgc caacggaaac cgttttagtt atcggcataa tgccgccaac
     1561 aataattcgg tcaatgtggc tgtaaaccat ggtgtactca tcggcgacaa ataccttttc
     1621 aaccaaaaat tcattgcgca gcccttgggt atccagcgtt tttgcgtgcg cactgtggat
     1681 gctctgtctt acgtccacat caacctccga taatcagtga agcgtgaaaa tagaaaacga
     1741 tagtgaaaca ctgtttcgtt ttctctataa agggatgata tgcccatggg ctagggtttt
     1801 caattaaaaa taaaacaatg tttcattttt aagttaagga ttaaaaaaag tgcagtgtcg
     1861 atcacgaata tgtcatgaga atggaaaaat gagggagccc agaaaatgta tgtcgacttg
     1921 ccagccatca ttattcaaca gattaatgac ggcagatatt tatcggtaag gaggaaatgc
     1981 agagctattc gtcacgttca atggtcaatg ccacaccctg gcccccgccg atacaaagcg
     2041 ttgccagtcc tttgcgggca ttacgtttca ccatttcatg aaccagagaa accaggattc
     2101 ggcaaccgga agcgcctatc gggtgaccaa gtgcgatcgc accaccattg acattgaccc
     2161 gacgctcatc ccactcaagc atcttgccaa ccgaaagcgc ctgtgcagca aacgcttcat
     2221 tagcctcgat aagatcgaca tcagccaact gccagcctac acgctccagg caacggcggg
     2281 tcgcatacac cggcgcaatt cccatcaatg ccggatctac accaacgctg gcaaatgcgc
     2341 gaatgcgggc cagcacgggt aaattcaacg ctcgtgcttt ggcttcgctc atcatcatta
     2401 ccgcagctgc gccatcgttt atggatgatg cattacccgc tgtcacagaa ccgagactat
     2461 caaatgaagg atttaaacgg gctaaacctt ctgcactggc gtcagtgcgt ggctgttcat
     2521 cggtatcaac aaccaacgtc tgaccgttac tttgggtcat taccgggacg atctcatctt
     2581 taaatcgtcc ggcgtcaatc gccgctcgcg ctttttgttg cgagctaagt gcgtaagcat
     2641 cctgcaattg acggctgatg ccatattcgc gagccagatt ttcggcggtg acaccaatat
     2701 gataatcatt gaaggcatcc cataacccat catgcacaag actgtcaacc agctggctat
     2761 tgccaagctg tgcaccggtg cggctatcag tcagaacatg tggtgcgcgg ctcatgtttt
     2821 cctggccacc ggcgatgaca atatcagcct cgccacactg tatcgcctga gtagccagat
     2881 gcagtgcttt aagtccggaa ccgcagacgt cattaatcgt gattgcagaa acgctattag
     2941 gcagaccacc tttaatagcc gattgccttg ccggattctg ccctgcccct gcagtcaaca
     3001 cctgaccaag aattacttca tccaccgcat atgcaggaac gccggtacgt tctattaacg
     3061 ctttcacgac cagactacca agttccacgg cggaatgacc cgctaacgca ccgcgaaagc
     3121 agccgatagg tgtccgtaac gccccgacaa tcacaacgtc tttcatcaca acctcgcacc
     3181 aattaacagt gcaatagtaa atgattgtta attacagtta tcttaattgt ttaaaaaaag
     3241 tgatttttat cacaaaggaa atatgcctga gcagtagtca gagtcataac tggcacgtaa
     3301 ggtttgcaac cactaaccca ccaatagagg ggtagatagg gcgttaatct cccatactta
     3361 acctggttta tggtaaattg ccctccattt tgtttaattt gtagatgata cgttcagata
     3421 atgtctaata tttggtctaa agaagaaact ctgtggagtt tcgcgctcta cggcacagcc
     3481 gttggtgcag gcacgctctt ccttcctatt cagttaggtt cggcaggggc tgtggtcctg
     3541 tttattactg ctctggtcgc ctggccttta acatattggc cacataaagc cttatgccag
     3601 ttcatcctct catcgaaaac atcagcaggt gaagggataa cgggcgcagt aacacactac
     3661 tatggcaaga agattggtaa tctgattacc acgctgtact tcatcgcctt ttttgtcgtc
     3721 gtgttgatat atgcagtggc aattaccaac tcacttacgg aacagctggc aaagcatatg
     3781 gttattgatc ttcgcatccg tatgttggtg agtctgggtg ttgtattaat cctgaatctc
     3841 atttttctga tgggacgcca tgcaaccatt cgggtaatgg gttttttggt attcccattg
     3901 attgcctatt tcttatttct ttccatttac cttgtcggta gttggcaacc tgatctatta
     3961 acgacccagg tagagttcaa tcagaatacc cttcaccaga tatggatatc gattcccgtg
     4021 atggttttcg cctttagcca tacgcccatt atttctacgt ttgccataga cagacgtgaa
     4081 aaatatggcg aacacgctat ggataaatgc aaaaaaatta tgaaagtcgc ttatctcatc
     4141 atctgcataa gtgtactgtt ctttgtcttt agctgcctgc tttctattcc accttcgtat
     4201 attgaagcgg ctaaagaaga aggggtcacc attttatcgg cgctttctat gctgccgaac
     4261 gccccagcat ggctgtcaat ttccgggatt attgtcgcag tagttgcgat gtcgaaatca
     4321 ttcctgggta cgtattttgg cgttattgaa ggtgccacgg aggtcgtcaa aacaacacta
     4381 cagcaggttg gtgtaaagaa aagtcgtgca tttaaccgcg cactatcaat tatgttggta
     4441 tcgctgatca ccttcattgt ttgttgcatt aacccgaacg cgatttcgat gatttacgcg
     4501 atcagcggcc cgctcattgc catgatactt ttcatcatgc ctacgttgtc aacgtatctg
     4561 atcccggcgc ttaaaccctg gcgttccatc ggaaatctga ttacgctgat cgtgggtatc
     4621 ctgtgcgtat cggtaatgtt ctttagctga tatccattgt gaatggttca aaattgctac
     4681 aattttgaac cattccagtt ttcataatgt tcattcgtta ttattccgca ctatcacttc
     4741 cctcatcata tcctagtatt atcagttagt taaacacaca aagagatctg aacatcttaa
     4801 ataagactat ttaagatgca taacttagat tcgcaagata tcttccggtc attcaaaaac
     4861 aatttgcgat atagatattc tcataccatg agatagttat ctgaaccacc ctcaaccagg
     4921 taagattaaa tcagtgaaac ataatcatat ttaataacga gaaatgcatt ttaaaatgtc
     4981 ttaatcagaa ttatagcaaa tacagattaa tcgcaatttt tcccagcaaa attacaaaat
     5041 catggtgtat tgacatcatc aatcaccaat gaaattatgc aattatatac ggatagggag
     5101 gttcttaaca tggggctttg tagtcgttat aaaagtctta catgcaatag ttgctcaatg
     5161 cattgccaaa taatgccaga agagtcaccg cgtttgcagt actgcgcaaa ctcgtgtttt
     5221 tgtatgtggc ccgaagaaag ttcatatttt aatcgtggtg tggtagaagg tatcttaaca
     5281 aaaaaccaca atgcgagatt aagcggatat atttttgtcg atttttcagt aagtttttta
     5341 cgtctattcc ttgaaaagga ctggattgac tatcttgcaa gtactgacat ggggattgtt
     5401 ttagtcagcg accgcaatat gcaatcgtta gctaattact ggcgaaaaca caactcagca
     5461 atatctgctg tcatatataa tgatgatggt cttgatgttg ccaatgaaaa gatcagacaa
     5521 ctatttattg gtcgttattt atcatttacc agaggtaaca cattaactca gatggaattc
     5581 accataatgg ggtatatggt ttctggttat aatacatatc agattgctga agttctggat
     5641 atggatatcc gtagcatcta cgcgtacaag caacgaatcg aaaagagaat gggtgataaa
     5701 ataaacgaat tatttattcg ttcacattcg gtccaacatt gatactcaac gaccaggcag
     5761 aatcatactc tgtttatacg ggcatttatt gaaatgtccg tattttataa ataggccgtc
     5821 ccctcaagag taaacaccat agtatttaaa accatcacac gaaaaattca gaagcattac
     5881 gaaatatgaa tttacaaaca atagtggcat aaatgttaac catgttaatt tacgtaaagt
     5941 tttacgttgc aacattaaag cctcatttca atcatcatga taaatataaa attaatatat
     6001 atttatgccg taaataccaa tatacttagc aaactatgta atctccattt cgattgattt
     6061 agtgtttatt gacgtatgta ctggattatt aacgataata tcgagttctg gcctgagcac
     6121 cgaaaattaa tatcggtaca taacgccgat cttaacgtcg ttctgacaac gccagccagt
     6181 cgatgtttat cacttctact tgaagctttt cctgatgtgg ttgcacaaca agattttttc
     6241 accagagtct gggaagaaga aggtatgcgt gtgcctacta acacgttata tcagaacata
     6301 tccattatca gacgcggatt tcgcgctgtt ggtgatacta cccactcgct aattgcaacc
     6361 gtgccgagaa gaggattcaa gatccataat gacattaaca tacaaaatca tgtaataaac
     6421 tcgtcaacag acgcacatac acacaatgcc ccacctgcca taaaagttaa tgcgggatac
     6481 aaagagagca ttggtggcgc aaagaatttc aataacaaaa tcctcaaaca tataaaaagt
     6541 catctaatta tgttgagcgc atttgtcata ggcgcatatt ctgcatattg gctatggaat
     6601 aataatcaac cgaagccatt tttcaaggat tacaagactg ttgcggaaat taatggctgc
     6661 cattttaatg taacagacga tacaattgat gggttgaaag agttcgataa atataaaaca
     6721 cggatactgg attcggggat taactgtaaa aaacatccgt ggttatactt ccctcttgca
     6781 aaatcctcac cggggatgat tgttatggca tgcaataaaa actataacca acatgaagtg
     6841 gcagactgtt taaccctgtc ttaccgtgag gttaatcgtg attgattata aaaaaaactt
     6901 attatttatt ttagttttta tcagtgggtt tattctcttc actgtctatt cttacactgc
     6961 agaaaaaatg atatataacg aaacatgcac tgcaaattgg gtaattttta atgaccaggg
     7021 acgagcaaat cttaccatcg actttatgta taacaaaaaa aataaaacag gaactgttgc
     7081 gttgagtgga acctggcaac aaggtaacag agaaagtaaa tcaatacggc gaaatattga
     7141 atacacatgg attgaaaact atgacacagc ccatttaaca tcaaagaaag ttaataagtt
     7201 tgagattatg gatcaagttg atgatgatag acttgcacaa cttattcctg atttttatgt
     7261 ctttccagaa aaaagtgtaa gctataatat actaaagcaa ggtaagcatg cttttatttt
     7321 gagcattggt aacagagcaa taatgcattg tgcaaggtaa actaaaatag ataacaaaaa
     7381 gatggaacct cgttaatcat aaaacaataa aattaaatat tctcgcagta tatggcagtc
     7441 taaagcatca aagttttgat caacatcttt cattttagac atctccttgc aatgtaaaat
     7501 atcatgtatc aacaacatct ggtgcaaatc catgagtcga actcgacatt catcttagct
     7561 cgattattat tccttcgtac agtcgatgta aacaatacag aaagaggatt attaagaacc
     7621 agtttactca gtgaatcaat agaggaaagg actaacgttt ctttaaaaga attgatttca
     7681 tcatctgtta aactaaactc atcattgaca gatcgtgaga tataactgtt tttaacttta
     7741 ctctttacgt tgactttatt gacagaatta acattcacat atcttgattt taatgtccat
     7801 gatgttgttt gcgtgagaac attctcagca ttaaggaatt tttttaacgg aatccttggt
     7861 ttctttttcg agtccgaatt tacaatatca tgcatataga ccatttcatg aatctgcgaa
     7921 aattcaatgt ccattgtata cctcacattt ttaccgtgac tcgatgttac tgttcaataa
     7981 tcaccttcca tcaatactaa aattaatacc cctaatgtgc cgataacaaa tatagtcatt
     8041 ctacgtaacg tctccataag gtgatatttg acattatcag aagttgcgaa ttcggatttt
     8101 gctctaatca aatgagagaa atgtaaaatg aagccacgaa atattaataa tagcctacca
     8161 ctgcaaccat tagttcctga tcaggagaac aaaaataaga aaaatgaaga gaaatccgtt
     8221 aatccagtta aaatcacaat ggggtctggt ttaaattata ttgaacaaga atctcttgga
     8281 ggaaaatatc taacacatga tttgtcaata aagatagcgg atatttctga agagataatt
     8341 cagcaagcaa tattatctgc tatgagcata tataaatttt cgataacaga tgatttaatg
     8401 agtatggctg taaatgaact cataaaactg accaaaatag agaataatgt agacctgaat
     8461 aaattcacta ctatatgcac agacgttcta tccccccg
//
LOCUS       DAJOWB010000172         8460 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJOWB010000172 DAJOWB010000000
VERSION     DAJOWB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.172"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..2187
                     /locus_tag="OHO85_004357"
     CDS             <1..2187
                     /locus_tag="OHO85_004357"
                     /inference="COORDINATES: protein motif:HMM:NF018353.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCQ2673627.1"
                     /translation="EFCNRYEKGGKDGITMTFIGPSPSVQDKPASTDNTNINGEDMTE
                     IEESMLLPVSGQELPIRWLAQHGSEKPVTHVSRDELQALHIARAEELPAVTALAISHK
                     TSLLDSLEIRDLHKLVRDTDKVFPNPGNSDLGLITAFFEAYLDADYIDRGLLTKEWMK
                     GNRVSRITRTASGANAGGGNKTDRNPNLVHTLDTLDVEIAAATLPMDFNIYEIPGSVY
                     RRAKEVVLKKESPFKEWSAALRATPGILDYSRAAIFALIRSAHPEFYHYPGRLQGYIN
                     AYLTETDHENPSKETLTAARHTPEKDILEEINREVVTERETEEEKPQPSDAMAGEQAT
                     TETMEPDTTEHCQNVQSQVSSANQVKVTADEVNKIMQAANISQPDADKLLAVSRGEFV
                     EGISDPNDPKWVKGIQTRDSVNQNQHESERNDQKAEQNSPNALQNEPETKQSEPVAQQ
                     EPEKVCTACGQRGGGNCPDCGAVMGDATYQETFDEENQVEVQENVPEEMEGAEHPHKE
                     NPGGNQHHASDNKTGEATDPLIKVNGHHKLTSTSRAGIHLMIDLETMGKNPDAPIISI
                     GAIFFDPQTGDMGPEFSKTIDLDTAGGVIDRDVIKWWLKQSREAQSAIMTDEIPLDDA
                     LLQLREFIDENSGEFFVRVWGNGANFDNVILRRSYERQGIPCPWRYCNDRDVRTIVEL
                     GKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIPSQADF"
     gene            2246..2449
                     /locus_tag="OHO85_004358"
     CDS             2246..2449
                     /locus_tag="OHO85_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HCQ2673628.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            2449..3474
                     /locus_tag="OHO85_004359"
     CDS             2449..3474
                     /locus_tag="OHO85_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2673629.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEKSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQEVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPKSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(3527..3616)
                     /locus_tag="OHO85_004360"
     tRNA            complement(3527..3616)
                     /locus_tag="OHO85_004360"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3580..3582),aa:Ser,seq:cga)
     gene            3710..4507
                     /gene="mtfA"
                     /locus_tag="OHO85_004361"
     CDS             3710..4507
                     /gene="mtfA"
                     /locus_tag="OHO85_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCQ2673630.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRTCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            4608..4683
                     /locus_tag="OHO85_004362"
     tRNA            4608..4683
                     /locus_tag="OHO85_004362"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4641..4643,aa:Asn,seq:gtt)
     gene            4845..6101
                     /locus_tag="OHO85_004363"
     CDS             4845..6101
                     /locus_tag="OHO85_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2673631.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     NGKESRIALGAYPAVSLSDARQQREGVRKMLALNINPVQQRAAERGSRTPDKVFKNVA
                     LAWHKSNRKWSQNTADRLLASMNNHIFPVIGNLPVSELKPRHFIDLLKRIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQGLIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIEA
                     YHQGRELTRLAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLDICRKAYVSPYMMVQENR"
     gene            6286..8259
                     /locus_tag="OHO85_004364"
     CDS             6286..8259
                     /locus_tag="OHO85_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HCQ2673632.1"
                     /translation="MSSPLKSVFKKEHSDEISNHSVNPVFSEVVSAFMSRRRFLQMGM
                     VAGAAVSFPYLVKPENAFAAKANPSALSKAVSLGFTSIPVSTADTVTVPEGYIARPFY
                     RWGDAVGIKGNLPEFKFDASNTTDEQAAQAGMHHDGMAWFSLPQGKENPAHGLLALNH
                     EYIDNGMLFKDGTANWDLDKARKGQNAMGISVIEVKKDNVGWQVVRPSSFARRITVNT
                     PMQLSGPARHQALMKTAADPQGEVVLGTMQNCANGKTPWGTYLTCEENWSDIFVKKAP
                     RNVLEKRYGISDSDESYRWNEVDERFSVDKTPNEPNRFGWVVEIDPYDPTSTPRKHTA
                     LGRFKHEGAAVTLAGDNRVVVYMGDDQKFEYIYKFISENKYDPGDRKANMQLLESGTL
                     YVARFNDDGSGDWLPLIFGENGLDQSKGFDNQGDLLIKTRLAADTVGATKMDRPEWIA
                     VDTHAKGSVYCTLTNNSDRGKEGKAPVDAANPRANNQFGHIMHWREERADPASAKFTW
                     NILVLAGRTDSDDPKAKGSMQGAEFGSPDGLSFDHRGVLWIQTDVSSSTINKKAYEGM
                     GNNQMIATLPGTNEYRRFLTGPRGCEITGIAFTPDNRTLFINIQHPGEGGDDITDPSN
                     PRAISNWPDSRSDGRPRSSTVVITKSNGGIIGT"
BASE COUNT         2340 a         2044 c         2094 g         1982 t
ORIGIN      
        1 gaattctgca accgctatga aaaaggcggg aaagatggca tcacaatgac atttatcggc
       61 ccttccccct ctgttcagga caaaccagcc agcactgaca ataccaacat caacggcgaa
      121 gacatgactg agattgagga gagcatgctt ctgcctgtct ccggtcagga actgcccatt
      181 cgttggcttg ctcaacacgg cagcgaaaaa ccagtaacgc acgtttcacg cgacgaactc
      241 caggcattac acattgcacg ggctgaagaa ctaccggctg ttactgctct ggctatttcg
      301 cataaaacca gtctgctcga ctcgctggag attcgcgacc tccacaaact ggttcgtgac
      361 actgacaaag ttttccctaa tcctggtaat tcagacctgg gactaataac tgcttttttc
      421 gaagcatacc tggacgctga ctacattgat cggggtctgc tgacaaaaga gtggatgaaa
      481 ggaaatcgtg tttcacgcat cacccgcacg gcttccggtg ctaatgctgg cggtgggaac
      541 aaaaccgatc gcaatccgaa tttagtacac accctcgaca cactggatgt ggagattgca
      601 gcagccacac ttccgatgga ttttaatatt tatgaaattc cgggcagcgt ttatcgtcgc
      661 gcaaaagaag tagtcctgaa aaaagaaagt ccgttcaaag aatggtccgc agcacttcgt
      721 gcaaccccgg gtattctgga ctattcccgc gccgctattt ttgcacttat ccgaagcgca
      781 caccctgaat tttatcacta cccgggacgc cttcaggggt atatcaacgc ctatttgacc
      841 gaaactgatc acgagaaccc cagcaaggaa actctcacag ctgcccggca tacgccggaa
      901 aaagatatcc tggaagaaat taaccgcgag gtggttactg aacgtgaaac agaggaagaa
      961 aaaccacaac catctgacgc aatggcaggt gaacaggcaa caactgaaac aatggaaccg
     1021 gatacaactg aacattgcca gaacgttcag tcgcaggtga gttccgctaa ccaagtaaaa
     1081 gtcaccgctg acgaagtaaa caaaattatg caggcagcca atatcagcca gcctgacgcc
     1141 gataagttac ttgctgtatc gcgtggtgaa tttgttgagg ggattagcga ccctaatgat
     1201 ccgaaatggg tcaaggggat ccagactcgc gattctgtga accagaacca gcatgaatcg
     1261 gaacggaacg accaaaaagc ggaacaaaac agcccaaatg cgttacaaaa cgagccagaa
     1321 acgaaacaat ccgaaccagt agcgcaacag gaaccggaaa aagtctgcac cgcctgcggt
     1381 cagaggggtg gcggcaactg ccctgattgt ggcgcggtga tgggcgacgc aacataccag
     1441 gaaacattcg atgaagagaa tcaggttgaa gttcaggaaa atgttccgga ggaaatggaa
     1501 ggcgctgaac atccacacaa ggagaaccct ggcggcaatc agcatcacgc cagcgataat
     1561 aaaactggcg aggcgacaga tcccttaatt aaggtgaatg gtcatcataa gctcacatcc
     1621 accagcagag cggggattca tctgatgatc gaccttgaaa ccatgggaaa aaatcccgat
     1681 gccccgatta tctcaatagg cgcaatattt ttcgatccac aaaccggaga tatgggaccg
     1741 gaatttagca agaccatcga tctggatact gctggcggag tcattgatcg tgacgtcatt
     1801 aaatggtggc tgaagcaatc acgtgaggcg cagtctgcca ttatgaccga tgaaatcccg
     1861 ttagatgatg cactactgca attgcgggaa tttatcgacg aaaactccgg tgaatttttt
     1921 gttcgggtct ggggtaatgg ggccaacttc gacaacgtga ttttacgccg ttcatacgaa
     1981 cgacagggta tcccctgccc gtggcgctac tgcaacgatc gcgatgtacg cacaatcgtt
     2041 gagctgggga aagccataga cttcgatgcc agaactgcta tcccattcga aggtgagcgc
     2101 cataatgcac ttgatgacgc tcgttaccag gcaaaatacg tttcagctat ctggcaaaaa
     2161 ctgatcccga gtcaggctga tttttaatgt tcaaccctaa ttgccgctaa ccgtatatag
     2221 ttagcggcgg ttatgagata tagctatgag cagcttattt ttaaccgaag atgaattgct
     2281 aatattaacg ggctgcaaat atgcaagcca ccagcgaaaa tggttaatgg aaaacgggct
     2341 tccgttctat accaatcgta gtggcaaacc gattgtcagc cgggatctat ttacctgcaa
     2401 taaaacttta ccaccacgcg aggtagagcc gaattttggt gcgatctgat gggaagacga
     2461 aggaaaaatc ctgaacacga aaaattacct ccaaatgtat acccaaataa atatagttat
     2521 gtatggaaac caacatccag agaatctgtc acactaaccg ccatcaagga tggtttagct
     2581 gctttatgga aaaagtatga ggaaactgta aataatcgcg atcgtgcaat gacattcggt
     2641 cgcttgtggg aaaaattcct cgccagcgcc tattacagtg accttagtcc aagaacacaa
     2701 aaagattatc tgcaacatca aaaaaagttg cttgccgtat tcggtaaggt accagcggat
     2761 tccataaaac cagaacacat ccgtcgatac atggacaaaa gaggggagaa gagtaaaacg
     2821 caagccaacc atgaaaaaag cagtatgtcc cgtgtttaca gttgggggta tgagcgaggg
     2881 tacgtgaagg ctaacccatg tgcaggtgta agtaaattca aggccaaaaa ccgcgaacga
     2941 tatgtaaccg acaaagaata ccaggaagta ttaagcgttg cacctcttcc tgtttttatc
     3001 gcaatggaaa ttgcctatct gtgtgcagcg agggtttccg atgtgttatc gctgaaatgg
     3061 gaacagattg gaaacgacgg gatattcatc cagcaaggga aaaccggaaa aaaacagata
     3121 aaagcatgga gtccacgatt acaggcagcg atcgaaaaag caaaacagtt accaaaatct
     3181 gcctatgtga tcagcaatca atacggcaac cgatatatgt acaaaggctt taacgaaatg
     3241 tgggtagaag caagaaatcg tgctggaaaa atttcaggta ttttaaccga cttcaccttt
     3301 catgatctga aggcgaaagg aatttcagac tatgaaggaa gcagccggga taagcaactt
     3361 ttctctggtc acaaaaccga agggcaagtg ctaatctatg acaggaaggt taaagtttca
     3421 ccaacacttg atgtcccgtt acctgaaaat attccaagaa aatattccaa gtaattccaa
     3481 gtgtgatttt tgtcactgac ttaatgatgt gtaagtgatt gaattttggc ggagagaggg
     3541 ggatttgaac ccccggtgga gttgccccca ctccggtttt cgagaccggt ccgttcagcc
     3601 gctccggcat ctctccgttc agatggttgc catgatgcca ggaaatttgg cattttaaca
     3661 gtccctgtcc gtgcaatttt gttcaagtga cgagtttgcg agcaaaacga tgattaagtg
     3721 gccctggaaa gtacaagaat cagcacatca aactgccctt ccctggcagg aagcactatc
     3781 gatccccctt ttaacgtgtc tgacggaaca ggaacaaagc aaattagtca ctcttgccga
     3841 acgtttttta cagcaaaagc ggcttgttcc tttacagggc tttgaactgg attcattaag
     3901 aacctgccgg atagcacttc tattttgcct acccgttctg gaattaggac tggaatggct
     3961 ggatggtttt catgaagtct taatttatcc tgcgccattt gtggtcgatg atgaatggga
     4021 agacgatatc ggtctggtgc ataaccaacg tattgttcag tcaggtcaga gctggcagca
     4081 agggcctatc gttttgaact ggttggatat acaagattct tttgatgctt ctggttttaa
     4141 cctgattatt catgaagtcg ctcataagct ggacacccgt aacggcgatc gcgccagcgg
     4201 agttcccttt attccgttgc gtgaggttgc tggctgggaa cacgatcttc atgctgcaat
     4261 gaacaacatt caggaagaaa tcgaattggt tggcgagaat gcggcgagca ttgatgctta
     4321 tgctgccagt gatcctgctg aatgttttgc cgtactttct gaatatttct ttagcgcccc
     4381 agaacttttt gctcctcgtt tcccttcatt gtggcaacgt ttctgccaat tttatcaaca
     4441 agatcctttg cagagactgc atcacgctaa tgatacagac tcgttttcgg cgacgaatgt
     4501 tcattaatta acaactttgc agattaatta accaattgaa atgacttatg aaatttagtg
     4561 ttgacagaca aggtaccgct aagtaatatg cgccccgttc acacgattcc tctgtagttc
     4621 agtcggtaga acggcggact gttaatccgt atgtcactgg ttcgagtcca gtcagaggag
     4681 ccaatttgct gttttcatgc atccttgcga atcattatat gatgttggtt caacaggtta
     4741 gcgtgaaaac tcttcccggt gcattttgat tttaccctcc gcatcgggaa aaattggtgg
     4801 tcaaatctgg ggtcaggtta gttcgataat ggagcgaccc ccatatgtcc cttaccgacg
     4861 cgaaaatccg cactctcaag ccttctgata aaccctttaa agtctccgat tcccacggtc
     4921 tgtatctgct ggtcaagccg ggtggctcac gtcactggta tctcaagtac cgtattaacg
     4981 gtaaagaatc gcgcattgcg ctgggtgcct atcctgccgt ctccctctct gatgcgcgac
     5041 agcagcgtga aggtgtccgt aaaatgctgg cgctgaatat caacccggta cagcagcggg
     5101 ctgctgaacg tggctcacga acaccggata aagtttttaa aaacgtggcg ctggcgtggc
     5161 ataaaagtaa ccgaaaatgg tcgcagaaca ccgctgaccg tctgcttgcc agcatgaaca
     5221 atcacatctt tccggtcatt ggcaacctgc ctgtatcaga acttaaaccc cgtcatttca
     5281 ttgacctgct gaaaaggatc gaggaaaaag gtctgctgga agttgcgtcc cgcacgcggc
     5341 agcacctgag taacataatg cgccatgcgg ttcatcaggg gttaatcgat acgaacccgg
     5401 cagcaaatct tggcggcgtg accacacctc ctgtcagacg gcactatcct gccctgccac
     5461 tggagcggct gcctgaactg cttgaacgta ttgaggcata tcatcagggc cgtgaactga
     5521 cgcggttggc cgtcctgctg atgctgcatg tgttcattcg ctccagtgaa ctgcgtttcg
     5581 cccgctggtc agagattgat ttcacaaacc gagtctggac gatacccgcg acgcgagaac
     5641 ccattattgg agtgcgttat tccggccgcg gggccaaaat gcgaatgccg catatcgtac
     5701 ccctctcaga acagtccatc gcaattctga aacagattaa ggatatcacc ggtaataatg
     5761 aactgatctt ccccggcgac cataacccgt ataagccaat gtgtgaaaac acggtcaata
     5821 aggcactgcg ggtgatgggc tacgacacga aaaaggatat ctgtggccac ggctttcggg
     5881 cgatggcatg cagtgcgctg atggaatcgg gtttatgggc aaaggatgca gtagaacgcc
     5941 agatgagtca tcaggagcgc aataccgtgc gcatggctta tattcacaag gcagaacact
     6001 tggaagcccg caaggcgatg atgcagtggt ggtcggacta tctggatata tgcagaaaag
     6061 catatgtgtc gccttatatg atggttcagg agaaccgctg aattgttagt ataacgaagc
     6121 cgtctccgct taggtgggga cggctttatt tatcagtaat acaaaaatgc cagtgagctt
     6181 tttctcctga acctccgtta tttctcatgt tataaatttg taacaaatat tttttaaact
     6241 gtcctgtatt gccacgcaaa tctatggaaa ttacaggaaa acgatatgag cagccccctt
     6301 aaatcggtgt ttaaaaagga gcacagcgat gagatcagta accacagcgt gaaccccgtc
     6361 ttttctgagg tagtcagcgc ttttatgtcc cgtcgccgct ttctgcaaat gggaatggtg
     6421 gcgggcgctg ccgtctcctt tccttatcta gtcaagccag aaaacgcctt cgcggcgaag
     6481 gcaaatccct cagcattatc taaagcggtg tcactgggct tcaccagcat tcccgtttcg
     6541 acagcagaca ctgtcaccgt gccggaaggc tatatagccc gtccgtttta tcgctggggc
     6601 gatgctgtgg gcataaaggg taatctgccg gaatttaaat tcgacgctag caacaccacc
     6661 gacgaacagg cggcgcaggc cggtatgcac catgacggca tggcctggtt tagcctgccg
     6721 cagggcaaag agaacccggc acacggtctg ctggcgctca accatgagta cattgataac
     6781 ggcatgctgt ttaaagacgg taccgccaac tgggatcttg ataaggcccg taagggtcag
     6841 aatgcgatgg gtatatcagt tatcgaggtg aaaaaagata acgtgggctg gcaggtagtc
     6901 cgtccctcca gctttgctcg tcgcattacc gttaatacac caatgcaact gagcggtccg
     6961 gcgcgtcatc aggcgttgat gaaaaccgcc gccgatccgc aaggcgaagt ggtactgggc
     7021 accatgcaaa actgcgccaa tggtaaaacc ccgtggggga cgtacctgac ctgtgaagag
     7081 aactggtcgg atatttttgt taaaaaagcg ccgcgtaacg tgctggagaa gcgctacggc
     7141 attagcgata gcgatgaatc ctaccgctgg aacgaggttg acgaacgttt cagcgtcgat
     7201 aaaacgccca acgagccgaa ccgctttggc tgggtggtgg agattgaccc ctacgatccg
     7261 acctccacac cgcgcaaaca taccgcatta ggtcgcttta agcatgaagg tgccgccgtg
     7321 acgctggcgg gggataatcg cgttgtggtc tatatgggcg acgatcagaa atttgaatac
     7381 atctataaat tcatctccga gaacaaatat gaccctggcg atcgcaaggc aaatatgcag
     7441 ttgctggagt caggcacgct ctatgtcgcc agattcaatg acgacggttc cggcgactgg
     7501 ctgccgctaa tcttcggtga aaatggcctt gatcaaagca agggtttcga caatcagggc
     7561 gatctgctga tcaaaacgcg ccttgctgcc gatacggtgg gcgcaacaaa aatggatcgc
     7621 ccggaatgga tagccgttga tacccatgcg aaaggcagcg tgtattgcac gctcaccaac
     7681 aattccgatc gcggtaaaga gggcaaagcc ccggttgacg cagccaaccc gcgcgccaac
     7741 aaccagttcg gccatatcat gcactggcgc gaagagaggg cagaccccgc ctcagcgaaa
     7801 tttacgtgga atattctggt gctcgccggg cgtactgaca gtgacgatcc taaagcgaaa
     7861 ggctccatgc agggggcaga attcggcagc cctgatgggc tctccttcga tcaccgcggc
     7921 gtgctgtgga tccagactga tgtttcctcc agtacgatta ataaaaaggc gtatgaaggg
     7981 atggggaata accagatgat agcgaccctg cccggcacca atgagtatcg ccgctttttg
     8041 accgggccac gcgggtgtga aattaccggt atcgccttta cgccggacaa ccgtacgctg
     8101 tttattaata tccagcatcc cggcgaaggc ggcgacgaca ttaccgatcc gagcaaccct
     8161 cgggctatct ccaactggcc agattcaaga tcagacggac gaccacgttc ttcaacagtc
     8221 gttatcacta aatcgaatgg cgggataatc ggtacctgat gcatctggtg cccttttggg
     8281 caccattttt tatggcaaca gcttgatcct cccaacattc cctaggtatc aaatatttaa
     8341 tctcgctgtt atctgaagga attaacctta gccttttatg ttttctctta ctcgctgaaa
     8401 cgattatcaa ttttaacgct accttaagcc tggcttcgta catgcacgtt ccatatcaaa
//
LOCUS       DAJOWB010000173         8337 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJOWB010000173 DAJOWB010000000
VERSION     DAJOWB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.173"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(148..354)
                     /locus_tag="OHO85_004365"
     CDS             complement(148..354)
                     /locus_tag="OHO85_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCQ2673633.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            667..756
                     /gene="kdpF"
                     /locus_tag="OHO85_004366"
     CDS             667..756
                     /gene="kdpF"
                     /locus_tag="OHO85_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCQ2673634.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            756..2429
                     /gene="kdpA"
                     /locus_tag="OHO85_004367"
     CDS             756..2429
                     /gene="kdpA"
                     /locus_tag="OHO85_004367"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCQ2673635.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2452..4500
                     /gene="kdpB"
                     /locus_tag="OHO85_004368"
     CDS             2452..4500
                     /gene="kdpB"
                     /locus_tag="OHO85_004368"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCQ2673636.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMCLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4509..5081
                     /gene="kdpC"
                     /locus_tag="OHO85_004369"
     CDS             4509..5081
                     /gene="kdpC"
                     /locus_tag="OHO85_004369"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCQ2673637.1"
                     /translation="MSGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVINIVELNLALDKLDE"
     gene            5074..7758
                     /gene="kdpD"
                     /locus_tag="OHO85_004370"
     CDS             5074..7758
                     /gene="kdpD"
                     /locus_tag="OHO85_004370"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCQ2673638.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRTQGLDIVVGVVETHGRKDTAAMLEELAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7755..>8337
                     /gene="kdpE"
                     /locus_tag="OHO85_004371"
     CDS             7755..>8337
                     /gene="kdpE"
                     /locus_tag="OHO85_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCQ2673639.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRI"
BASE COUNT         1737 a         2164 c         2435 g         2001 t
ORIGIN      
        1 aacaattagg aaaatattta tatatatttc atgaatatat gtttcgattg gtataacgat
       61 gtctaaatat tcctctgaac atcttcctga tacaaaaaac cgccagcgtt tccgctggcg
      121 gttttcaatg tattcaaaac tcagcaatca ataaaaatca ccagttgcct tttcggcctg
      181 atccatccac accggtttat cgctggtttt cgaccagacg cgatgcaggt agctgtaaaa
      241 gcgggcgcgg tctttccaga aaagcatgaa aggcagcgcc agaacgcccg ctgcaaccgc
      301 ataagtacgg cgtaaaaaga taagccatgc aggatattct ctgtagagtt ccatactttt
      361 ctcccccttt tgtatctacc cggtgaatgg caccggaaaa atgaatttgt ttatctgatg
      421 aaaatagtac cgccttttgt gtaattttac tactcatccg accacttatt tttgcttatt
      481 gatggtttat ttacattcat cctgtaatta agttacacaa aagttaaatt aatactaaac
      541 attagttaaa tcatggcttt tgccattttt atactttttt tacaccccgc ccgcagattt
      601 ttgcgaaatc tttgcagcca gaattctacc cttccggtat cacttttagg ccactggagg
      661 tgcactgtga gtgcaggcgt gataaccggc gtattgctgg tgtttttatt actgggttat
      721 ctggtttatg ccctgatcaa tgcggaggcg ttctgatggc tgcgcaaggg ttcttactga
      781 tcgccacgtt tttactggtg ttaatggtgc tggcgcgtcc tttaggcagc gggctggcgc
      841 ggctgattaa tgacattcct cttcccggta caacgggcgt tgagcgcgta ctttttcgcg
      901 cacttggcgt ctctgaccgt gagatgaact ggaagcaata tctctgtgcc attctcggcc
      961 tgaacatgct ggggctggcg gtgctgtttt ttatgttgct cggtcagcac tatctgccgc
     1021 ttaatccaca gcagttgccg gggctgtcgt gggatctggc gctgaatacc gccgtcagct
     1081 ttgtcacgaa taccaactgg caatcttaca gcggtgaaac cacgttgagc tatttcagcc
     1141 agatggctgg cttaacggtg caaaactttc tttctgccgc cagcgggatt gcggtgattt
     1201 ttgccctcat ccgtgcgttt acccgccaga gcatgagcac actcgggaat gcctgggtcg
     1261 atctgctacg catcacgtta tgggtgctcg tccctgtggc gttgttgatc gcactgtttt
     1321 ttattcaaca aggtgcgctg caaaactttc agccttatca ggctgtgaat accgttgaag
     1381 gagcgcaaca gctgttaccc atggggcctg tagcttctca ggaagcgatc aagatgctcg
     1441 gtactaacgg cggtggcttc tttaatgcca actcgtcgca tccgtttgaa aacccaaccg
     1501 cactgaccaa cttcgtgcag atgctggcga tcttcttgat cccaacggcg ctgtgctttg
     1561 cctttggtga agtgacgggc gatcgccgcc aggggcgcat gttgctgtgg gcgatgtcag
     1621 tgatttttgt catctgcgta ggcgtggtga tgtgggcaga agttcagggt aatcctcatc
     1681 tgctggcgct cggcgcagac agcagcatca atatggaagg taaagagagc cgtttcggcg
     1741 tgctggtcag tagcctgttt gcggtcgtga ctacggcggc ttcctgtggc gcggtgattg
     1801 cgatgcatga ttcgtttacc gctctcggtg gcatggtgcc gatgtggctg atgcaaattg
     1861 gtgaagtggt gttcggcggt gtcggttctg gtctttacgg catgatgctg tttgtcctgc
     1921 tggcggtgtt tattgccggg ctgatgattg gtcgtacacc ggaatatctg ggtaaaaaaa
     1981 tcgacgtacg cgagatgaaa ctgactgcac tggcgattct ggttaccccg acgctggtgc
     2041 tgatgggcgc ggcgctggca atgatgaccg acgccggacg tagcgccatg ctcaaccctg
     2101 gcccgcatgg ttttagcgaa gtgctgtacg ccgtgtcatc cgccgctaat aacaacggca
     2161 gcgcctttgc cggattaagc gccaactctc cgttctggaa ctgtttactg gcgttctgca
     2221 tgtttgtcgg tcgcttcggg gtgattatcc cggtgatggc aattgccggt tcgctggtga
     2281 gtaaaaagag ccaacccgcc agctccggca cgctgccaac gcacggcccg ctgtttgttg
     2341 gcctgttaat cggcaccgtg ttgctggttg gcgcactgac ctttatccct gccctggcgc
     2401 ttggtccagt ggcggaatat ctctcctgat gatattgagt gagcactgaa tatgagtcgt
     2461 aaacaactgg cgctattcga accaacactt gtcgttcagg cgctgaaaga agcggtgaaa
     2521 aaattaaacc cgcaggcgca atggcgcaat ccggtgatgt ttatcgtctg gatcggcagt
     2581 ctgctgacca cctgtattag catcgcgatg gcaagcggtg cgatgcctgg caatgcgctg
     2641 tttagcgcgg caattagcgg ctggctgtgg gtcaccgtac tgttcgctaa tttcgccgag
     2701 gcgctggcag aaggccgcag taaagcgcag gccaacagtc ttaaaggggt gaaaaaaact
     2761 gcctttgccc gcaagctgcg tgagccgaaa tatggcgctg cggcggacaa agttcctgcc
     2821 gatcaactgc gtaaaggtga catcgtgctg gtagaagctg gcgatattat cccctgcgat
     2881 ggtgaagtca ttgaaggcgg cgcatcggtc gatgaaagcg ccatcaccgg ggaatcggca
     2941 ccggtgatcc gtgaatccgg cggcgatttt gcctccgtca ccggcggcac gcgtattctt
     3001 tctgactggc tggtgattga gtgtagcgtt aaccccggtg agacatttct ggatcggatg
     3061 atcgcgatgg tggaaggcgc acagcgacgc aaaacaccga acgagattgc cctgactatc
     3121 ctgctgattg ctctgaccat cgtcttttta ctggcaaccg ccacgctgtg gccgttttcc
     3181 gcgtggggcg gtaatgcagt cagcgtaacg gtactggtgg cgctgctggt ctgtctgatc
     3241 ccaaccacta ttggcggcct gttgtcagcg atcggcgtcg ccgggatgag ccggatgcta
     3301 ggcgcgaatg tgattgccac cagcggacgt gcagttgaag cggcaggtga cgttgacgtt
     3361 ctgctactgg ataaaaccgg caccatcaca ctcggtaacc gtcaggcgtc ggagtttatc
     3421 cccgcgcagg gcgtggatga aaaaacgctg gctgacgccg cacaactggc ttcgctggct
     3481 gatgaaacgc cggaaggccg cagtattgtg atcctcgcca agcagcgttt taacctgcgc
     3541 gagcgcgatg tgcagtcgct ccatgccacc tttgtaccgt ttactgcgca aagccggatg
     3601 agcgggatca acatcgacaa ccgcatgatc cgtaaaggtt ctgtcgatgc cattcgtcgc
     3661 catgttgagg ctaacggtgg tcacttccct accgatgttg atcaaaaagt cgatcaggtt
     3721 gcgcgtcagg gagccacgcc gctggtggtg gtggaaggtt ctcgtgtgct gggcgttatt
     3781 gcgctgaaag atatcgtcaa aggcggtatt aaagagcgct tcgcccagct gcgcaaaatg
     3841 ggcattaaaa cggtgatgat taccggcgat aaccgtctga ctgccgccgc gattgctgcg
     3901 gaagcgggtg tcgatgattt tctcgccgaa gcgacaccgg aggccaagct ggcattgatc
     3961 cgtcagtatc aggcggaagg tcgtttggta gcgatgaccg gcgacggcac caacgatgct
     4021 ccggcgctgg cgcaggcaga tgtcgcggtg gcgatgaact ccggcaccca ggcggcgaaa
     4081 gaggcgggca atatggtcga tctcgactct aacccgacca agttgatcga ggtggtgcac
     4141 attggcaaac agatgctgat gacccgtggc tcgctgacca ccttcagcat tgccaacgat
     4201 gtggcgaaat acttcgccat tattccggcg gcattcgcgg caacgtatcc gcagttaaat
     4261 gcgctgaaca tcatgtgcct gcattcgccc gactccgcaa tcctcagtgc ggtgattttc
     4321 aacgccttga ttatcgtctt tttgattccc ctggcgttaa aaggcgtgag ttataaaccg
     4381 cttaccgctt ctgccatgtt gcgccgtaac ttatggattt acggtctggg tgggctgctg
     4441 gtgccgttta tcggtatcaa agtcattgat ttactgctga ccgtttgcgg tctggtgtga
     4501 ggtttaccat gagtggatta cgtccggcat tatcaacatt tatctttctg ttattgatta
     4561 ctggcggcgt ttacccgctg ctgaccaccg tactggggca atggtggttt ccctggcagg
     4621 ccaatggttc gttgattcgt gaaggtgata cggtgcgcgg ttcggcatta atcgggcaga
     4681 attttaccgg caacggctat tttcatggtc gcccgtcggc aacggcagaa atgccctata
     4741 atccacaggc ttctggcggg agcaatctgg cggtcagtaa ccctgagctg gataaactaa
     4801 tagccgcacg cgttgctgca ttacgggccg ctaacccgga tgccagcgcg agcgttccgg
     4861 ttgaactggt gacggcatcg gcaagcgggc tggacaataa tatcaccccg caagcggcgg
     4921 cctggcaaat cccacgcgtg gcgaaagcgc gtaatctcag cgttgaacag ctcacgcaac
     4981 tgatcgcaaa atacagccaa caaccgctgg tgaaatatat cggccagccg gttatcaaca
     5041 ttgttgaact caatctggcg ctggataaac ttgatgaata acgaaccctt acgtcccgac
     5101 cccgatcgtc tgctggaaca aactgccgcg ccgcatcggg ggaagctgaa agttttcttc
     5161 ggtgcctgtg caggcgtcgg gaagacctgg gcgatgctgg cagaagcaca gcgactgcgg
     5221 acgcaagggc tggatattgt ggttggcgtg gtagaaaccc acgggcggaa agataccgcc
     5281 gccatgctgg aagagctggc tgttctgccg ccaaaacgcc tggcgtaccg tgggcggcat
     5341 atcagcgagt ttgatctcga tgccgccctc gcccgccgtc cggcgctgat cttaatggac
     5401 gaactggcgc acagtaatgc gcctggttct cgtcatccca aacgctggca ggatattgaa
     5461 gagctgctgg aagcaggcat tgatgttttt actaccgtca acgttcaaca tctggaaagc
     5521 ctgaatgatg tggtcagcgg cgtcaccgga attcaggtac gggaaaccgt gcccgatcct
     5581 tttttcgatg ccgccgacga cgtggttctg gtggacttgc ccccggacga tctgcgccag
     5641 cggctgaaag aaggcaaagt ctatattgcc gggcaggcgg agcgcgccat tgaacatttt
     5701 ttccgcaaag gcaatctgat cgccctgcgc gaactggcac tgcgccgtac tgccgatcgc
     5761 gtagatgagc aaatgcgcgc ctggcggggg catcctggcg aagagaaagt gtggcacacg
     5821 cgcgacgcga tccttttatg catcgggcat aacaccggca gtgagaaact ggtccgcgcg
     5881 gcggcgcggc tggcgtcgcg gctgggtagc gtctggcacg cggtgtatgt tgaaacccct
     5941 gccctgcatc gcttaccgga gaaaaaacgt cgggcaattc tcagcgcctt acgtctggcg
     6001 caggagctgg gcgcggagac ggcaacactt tctgatccag cggaagagaa agcggtagtg
     6061 cgttatgccc gtgaacataa tctcggcaag attattctcg gtcgcccagc ctcgcgccgc
     6121 tggtggcgtc gggaaacgtt tgctgaccga ctggcgcgca tcgcccccga tctcgatcag
     6181 gtgctggtcg cgcttgatga accacccgcc cgcacgatta acaacgcgcc ggatagccgc
     6241 tcttttaaag acaagtggcg ggtacagatt cagggatgcg tggttgccgc cgcgttatgc
     6301 gccgttatca ctttaattgc catgcagtgg ctgatggcgt ttgatgccgc caacctggtg
     6361 atgctgtatc tgcttggcgt ggtggtagtg gcgctatttt atggacgctg gccttcggtg
     6421 gttgctaccg tcattaatgt ggtgagtttc gatctctttt ttatcgcccc acgcggcacg
     6481 ctcgccgtct ctgatgtgca atatctgctg accttcgcgg tgatgttaac cgtcgggctg
     6541 gtgatcggga accttactgc tggcgtgcgt tatcaggcgc gggtagcccg ttaccgcgag
     6601 caacgcacac ggcacttata tgaaatgtcg aaagctctgg cggtgggccg cagtccgcag
     6661 gacatcgccg ccaccagcga acaatttatt gcctccacgt ttcatgcccg cagtcaggtg
     6721 ttgttgcccg atgacaacgg taaattgcag ccgttaacac atccgcaagg aatgacgcca
     6781 tgggacgatg ccatcgcgca gtggagtttt gataaaggcc tgcctgcggg tgcgggcacc
     6841 gacacgttac ccggcgtgcc gtaccagatt ttgccgctaa aaagcggcga gaaaacctac
     6901 ggactggtgg tggtggaacc ggggaatctg cgccagttga tgatcccgga gcagcagcgc
     6961 ctgctggaga catttacgct gttagtcgcc aatgcccttg agcggctgac actgaccgcc
     7021 agcgaagaac aggcgcggat ggcaagcgaa cgtgaacaga tccgcaacgc cctgctggcg
     7081 gcgctttcgc atgatttacg cacgccgctt acggtgctgt ttggtcaggc agaaatctta
     7141 acgctcgatc tggcaagcga aggatcgccc cacgcccgcc aggccagcga gattcgtcag
     7201 catgtgctga acactacccg actggtgaat aatctactgg atatggcgcg aattcagtcc
     7261 ggcggcttta atttgaagaa agagtggtta acgctggaag aagtggtcgg tagcgcgctg
     7321 caaatgctgg aaccgggttt atcgtcgccc atcaatcttt ctctgccaga accgctgacc
     7381 ttaatccacg ttgacgggcc actctttgaa cgggtgctga ttaatctgct ggagaacgcg
     7441 gtgaaatatg cgggtgcgca ggccgaaatt ggtatcgatg cccacgttga gggcgaaaat
     7501 ctacaactgg atgtctggga taacggcccc ggtcttccgc caggccagga gcagacgata
     7561 tttgataagt ttgctcgcgg gaataaagag tcggcagtac cgggggtagg gcttggactg
     7621 gcaatttgtc gggcgatagt ggatgtacac gggggcacta ttaccgcgtt caaccgaccg
     7681 gaaggtggtg cctgttttcg tgttacactt ccccagcaaa ccgctcctga acttgaagac
     7741 tttcatgagg atatgtgaca aacgttctga ttgttgaaga tgaacaggct attcgtcgct
     7801 ttctgcgcac ggcgctggag ggcgacggga tgcgcgtcta tgaggccgaa acgctgcaac
     7861 gcggcttgct ggaagcggct acccgtaagc cagatttgat tattctcgat ctcggcctgc
     7921 ccgatggtga tgggattgag tttatccgcg acctgcgcca gtggagcgcg gtgccggtga
     7981 ttgtgctttc cgcacgcagc gaagagagcg acaaaatcgc cgcgctggat gccggagcgg
     8041 atgattatct gagtaagccg tttggcattg gcgaattgca ggcccgtctg cgcgtcgctt
     8101 tacgccgcca ctccgccacc gccgcgcccg atccgctggt gaaattttcc gatgttaccg
     8161 tcgatttagc cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc acgccgattg
     8221 agttccgcct gctggcggtg ctgctgaata atgccgggaa agtgctcacc cagcgccagt
     8281 tactcaatca ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg cgtattt
//
LOCUS       DAJOWB010000174         8190 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJOWB010000174 DAJOWB010000000
VERSION     DAJOWB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.174"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(63..375)
                     /locus_tag="OHO85_004372"
                     /pseudo
     CDS             complement(63..375)
                     /locus_tag="OHO85_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(372..1274)
                     /locus_tag="OHO85_004373"
     CDS             complement(372..1274)
                     /locus_tag="OHO85_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCQ2673640.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVWFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPCGYDLNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            complement(1955..2347)
                     /locus_tag="OHO85_004374"
     CDS             complement(1955..2347)
                     /locus_tag="OHO85_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCQ2673641.1"
                     /translation="MYEGEERKKLSLYLHPEDSADCLALAEIETVPRKKRGELYRQAL
                     ITGLIMHQLDERIPAVLTALFTRELNADEVISQIARITGWKPSSGDLKEVLKALGGLQ
                     STVSPEHSQDDGEQARLKAARVKMQNLI"
     gene            complement(2351..3325)
                     /locus_tag="OHO85_004375"
     CDS             complement(2351..3325)
                     /locus_tag="OHO85_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StbA family protein"
                     /protein_id="HCQ2673642.1"
                     /translation="MRIFCDDGSTNVKLAWFEGKTLKSAVSVNSFRHNWKVEGLGSSR
                     TYNYLLDGRKYTYDPVSEDAISTTHIEYQYSDTNVLAVHHALLNSGIEPQEIDLTVTL
                     PISEFYTADCQKNTLNIERKIRNLMREVTLNKGGTFTIKSVEVMPESLPAVFTRLVAD
                     NVGQYEKSLVIDLGGTTLDVGVIVGQFEDVSAVHGNPDIGVSMVTKATLTALKMASSD
                     TSPMIADELIKNRNNLDFVGQVVNEVSKLNLVLDTIDTAINKLGELVVDDLLQYRNVN
                     RVYIVGGGAALIADAVRKAWNHLGEKVVLMDEPQTALVQAIARFKAEE"
     gene            complement(3564..3938)
                     /locus_tag="OHO85_004376"
     CDS             complement(3564..3938)
                     /locus_tag="OHO85_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673643.1"
                     /translation="MADASKLRTKRTRSGLGVPPGPDEVATSLNAPEIAPAVAATDVQ
                     RQQADSSPVHHEPVVTQTVTEVYTRRDGRSARKTNRTLAFATRVTPAFDQEIRDIAER
                     EGLKLVEVLEKAVEAYKEKQGY"
     gene            complement(3938..4570)
                     /locus_tag="OHO85_004377"
     CDS             complement(3938..4570)
                     /locus_tag="OHO85_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCQ2673644.1"
                     /translation="MAWIVGFISQKGGVGKSTKARALAREASACGIKTKLADLDLEQA
                     TSAEWHRRRLSAGLSPVASVEVFSTAKQAIESAGDFDLLILDGPARASKGTSEIAKVA
                     DLIVQPTGASLDDLVPAIKVFNALVKEGIPRSKLVFALSRVGTEAEELDARAYISEAG
                     YETLSGCLFEKPAYRKAMNSGLAVTETRYKGLNERADELIQALIDKIGEE"
     gene            complement(5154..5939)
                     /locus_tag="OHO85_004378"
     CDS             complement(5154..5939)
                     /locus_tag="OHO85_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HCQ2673645.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNNGARINEALALTRGDFSLAPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFAMPEADAVALIKQLERR"
     gene            complement(5941..6354)
                     /locus_tag="OHO85_004379"
     CDS             complement(5941..6354)
                     /locus_tag="OHO85_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673646.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGALHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            6923..7183
                     /locus_tag="OHO85_004380"
     CDS             6923..7183
                     /locus_tag="OHO85_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673647.1"
                     /translation="MDEERVFSLSYEQLTRFTERRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRKLIWPEDDWK"
     gene            7180..7770
                     /locus_tag="OHO85_004381"
     CDS             7180..7770
                     /locus_tag="OHO85_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673648.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLTEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLEVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGIEVRVQVNESVTP"
     gene            complement(7788..8135)
                     /locus_tag="OHO85_004382"
     CDS             complement(7788..8135)
                     /locus_tag="OHO85_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673649.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLCILWRLGIRLPPLPF
                     MPFWQVTLLMGSLWGISWGCAMWFIYRGPSGMVAGEAIIISITGGFLFGLLTASFHWW
                     RRKVNRLPPWGDV"
BASE COUNT         2031 a         2265 c         1999 g         1895 t
ORIGIN      
        1 tcttacagag ggcaccggga gggcacccct gtggtgcccg ttccccgctg cctgtgggct
       61 gttcaccgcg tccggtaacg ggtcaggccg accgtggaat accgcagccg tcgtctttct
      121 gacggcaagg ttatgccgcg aaagcggctt gaagggcgtt catcagaaaa gggcgacagg
      181 ctggcgtaca ctgtcaccga cgccagccgg gagcggtaaa agcgcccgtc ccgtgcccgg
      241 ccggacgcgg gacactgacc acaggcagcg tttatgccgc gccgcgtcag ccggcgcagt
      301 aaaaagagga gcacggcgac atcctttacc ggaaaagaaa cgggcggtag cccgcccgtc
      361 tccgtcatac ttcatccgcc ataatgcgcc accttgtcga ctatccacgc aggtccggtg
      421 acttccggca gctcatcgtc atctgtcggg gaagaccatt ccagctcccc ccacagcaca
      481 tcaacgagct ctccgcgctc atacaactgc cagccacaga aatcacagcc ctgttcggca
      541 taccagtgct ccagcgtgca gctgaaacgg cggcttaatt ctgcaataac gtcgctttcc
      601 ggctgacacc acggcgtgtc gaaatcaacc tgaatgaagt tgtctccctg actgctgata
      661 ttcaggtcat acccacaagg ccacttcacg ccatactgtt cggtatacca gtgatatgca
      721 gaaggaacac cgttcagaac accaccgtta aagccattca cttcaacatc aaggcgggtg
      781 ggcagaaccg tcagcaggtc aaacggcatg gttttttccg gcagtacgtt gtcacacaga
      841 cggttccacc acaccgatac atcctcgtta ctccagaagc cacaccagtc acctcttttt
      901 gcggtgaaaa gcgcggtgat ggtatccctg acatcgtccg gtaatccttc ccagcgacgc
      961 tggccagtac cgctctgcag ccagagttca tgcagcatca ggcagttttg ctcatccagc
     1021 agtacaccgt tctgcaggtg tgtcagccag cgggtgaacg cgatattctc cggcgataca
     1081 acgccacgtc cggcagccgt cagtccgggg cacggttcaa accacacatc ttcagtggtc
     1141 tgcagaagtc cggcacttcc ggccagaaac agctgaatac cttcatttgt ggcgcggcga
     1201 taaaacggtg tgacggcacc actggccagt cgtttaatct cagcaatctg tgccggttca
     1261 ccagaaaaat gcatacggtt actgcaccag tttggcatgg gaattctcct ctcacatgcg
     1321 gcctccatca ggccgtgccg cacctgtaac cgggcgaccg catacgaatg caggtgcggc
     1381 cccgaagccg cagcgcgtgg aggaggaacg acgaaacgtg accgggtgag gaggccggcg
     1441 acagcgacac acttgcatcg gatgcagccc ggttaacgtg ccggcacggc ctgggtaacc
     1501 aggtattttg tccacagaac cgtgcacaaa atgctgtgga taagctggac acagcactcc
     1561 acagcaggca tacagccata ccctcagaga attgagcgtt ttttagtcag aacaatgaca
     1621 tttgactgtg tgcggattca gttattagaa tactgtatat atatacagca aaagaaaagg
     1681 aagatgagaa catgatccgc attgaaattc ttttcgaccg ccagagcaca aaaaaactca
     1741 aatcaggcac acttcaggcg ctacagaatg aaatcgaaca acgtctgaaa ccacactacc
     1801 cggaaatctg gctgcatatg tgggaatccc cgtccttcag agtgaggagc tgtcaaccag
     1861 cactccattg actcaaatga cgggaaacgc ggaaatctaa aaattaagcc gcacaccccg
     1921 agaaattttg caggtttcac ggagcgagac ttttttagat caggttttgc atcttcaccc
     1981 tagccgcttt cagacgggcc tgttcaccgt catcctgaga gtgttctggt gaaactgtac
     2041 tctgaagccc tcccaacgcc ttcagtacct cttttaaatc accggaggag ggtttccagc
     2101 ctgttatcct ggcaatctga ctaatgacct catccgcatt caattctctg gtgaatagcg
     2161 cggttaacac ggcgggaatt cgctcatcaa gctgatgcat tatcaggccg gttatcaaag
     2221 cctgccgata taactccccc cgttttttcc ggggaacagt ttcgatttca gccagtgcaa
     2281 gacaatcggc agagtcctcc gggtgaaggt agagtgaaag ttttttcctt tcttcgcctt
     2341 cgtacatcga ttactcctcc gctttgaagc gtgcaatagc ctggaccagc gctgtctgag
     2401 gttcatccat aagaaccacc ttttcgccaa ggtggttcca tgccttacga acagcatcag
     2461 caatgagtgc agccccgcca ccgacaatat atacgcggtt tacgtttcga tactgcagca
     2521 aatcatcaac aactagctcg cccagtttat ttatcgctgt atcaatggta tccagcacca
     2581 gatttagttt agagacctca tttacaacct gccccacaaa atccaggtta ttcctgtttt
     2641 tgatcaactc gtcagcaatc attggactgg tatcactgct ggccattttc agtgcagtga
     2701 gcgtggcttt cgtgaccatt gatacaccaa tatcaggatt accatgtaca gccgaaacat
     2761 cctcaaactg gccaacaata accccgacat ccagagttgt accaccgaga tcgataacca
     2821 atgatttttc gtactgacca acgttgtcag caactaggcg agtaaataca gccgggagtg
     2881 attcaggcat cacttcaacg cttttaatgg taaaagttcc ccctttgttc agggtgactt
     2941 ctcgcatcag gttgcgaatt tttctctcaa tgttaagcgt atttttctgg cagtcggccg
     3001 tgtaaaactc gctgataggt aacgtaacgg tgagatcgat ctcctggggt tcaatgccac
     3061 tgttcagtaa tgcgtggtga actgccaaca catttgtatc agagtactga tactcaatat
     3121 gagtcgtgct gatcgcatct tcgctgaccg ggtcgtaggt atatttacgg ccatcaagca
     3181 gatagttata agtacgactg ctgcctaagc cctcgacttt ccagttatgg cgaaaagagt
     3241 tcactgatac cgcacttttc agcgtcttgc cttcaaacca ggcaagtttc acatttgtag
     3301 aaccatcatc gcaaaaaatt ctcataccat ctccatttat actcaacttg agtattcatc
     3361 tttacacaat aactactcac attaagtaaa tacgctcata aaccacaggt aagtactcaa
     3421 gctgcgtact tcacttgggc agttaactac tcaatttgag cattatatca tttatcttgc
     3481 agcaatctag catttatcta gcataaatct tgctgcaaga ttgcacacaa aaaaagaatg
     3541 cagcaagatt gctgcatccc ggattaatag ccctgttttt ccttatatgc ctcaactgct
     3601 ttttccagaa cttccaccag tttcaacccc tcacgctctg cgatatcccg gatctcttgg
     3661 tcgaaagcag gtgtgacacg tgttgcaaat gccagcgtcc tgtttgtttt tctggcagaa
     3721 cggccatcgc ggcgggtata tacctcggtc actgtttgcg taaccacagg ctcatggtgc
     3781 actggcgatg agtctgcctg ttgtctctga acatcagtgg cagcgacagc cggagctatc
     3841 tcaggcgcat tcaagctggt agccacttcg tcggggcccg ggggtacccc caacccacta
     3901 cgggttcttt tagttctaag ttttgacgca tcagccatta ctcttctcca atcttatcga
     3961 tgagtgcctg gataagttca tcagctctct cgtttaaacc tttataacgt gtttctgtca
     4021 cagcgagtcc tgaattcatc gcttttctgt atgctggctt ctcaaaaagg catccagata
     4081 aagtttcata acctgcttca ctgatatatg cacgagcatc cagctcttcc gcttcggtac
     4141 ctacccggct taaagcgaag accagttttg aacgtggtat accttctttt accaacgcgt
     4201 tgaaaacttt aattgccggg acaagatcgt caagggaggc tcccgttggc tggacgataa
     4261 ggtcggcgac cttagcaatt tctgaagtac ctttacttgc acgagcaggg ccatcaagaa
     4321 tcagaaggtc aaaatcgccg gcagattcta tcgcctgctt tgctgttgaa aacacttcaa
     4381 cggaagcaac cggtgacaaa ccagctgaca gacgtctacg atgccactca gcactcgttg
     4441 cctgttccaa gtcgagatca gctaattttg ttttgatacc acaggcggat gcttcacgcg
     4501 ctaacgctct cgctttagtg cttttgccca caccaccttt ctgagaaata aaaccaacta
     4561 tccaggccat ttagccccct ttacttgctg ctatcttgca gcaaacatca atataaacca
     4621 gattgagatt aggtagagta actcttccag aacaagataa cagcttgcag caagattgca
     4681 acaataaata atcttgctac ttagcagagt gctggaattg acagccgaat acttgtgtca
     4741 cgttaacaat aaaagaaacc tcattcagaa tcgcatcgac agatgaagca agattgccgc
     4801 aagattgcag atagcataac ataaacagag cgctaggccg tatgtgtaca gcatgatagc
     4861 tgcaatctat tttattgctg ctatcatgct gaaatatcat tattagaatc aggcagccct
     4921 cgccgcagcc atgtaactgt gcgtagcgag cgaactggcg aggaagcgtt ccggcagatg
     4981 gctcttacgc tcggcacaag aagagtttcc catccaggca agtactatgg gttgtcagcg
     5041 ccagagatat aagactgttc tggcctgaga acgtggcctc tatgagtttg gtgctctatc
     5101 ttccatagat gaatgcacca gctccccctt gactgaaccg cgaatacggc tttttaacgc
     5161 ctctcaagtt gttttatcag cgctaccgca tcggcttccg gcatggcgaa ctgcacacga
     5221 tgacgcgccg cgacatccag cgcaaagacc ttcgtgtaga cctccgttga gctgatggac
     5281 ttatgcccca tcagactctg caggaccttc agcggtatac cggcatacag catgtgcatc
     5341 gcataggaat ggcggaacgt atgtggcgtg accgggacag agaacgtcac tccatcagcg
     5401 gcagcggcgg caaccgcctc cccaatccag gtcctgaccg ttctgtccgt cacttcccag
     5461 atccgcgctt tctctgtcct gcctgttcgt ttattacgcc gttccatggg gattttcagt
     5521 gtggccacca tcgtctgcag ctggctgacg taccaggcgt cagagagcgg aaccagccgg
     5581 tgagtctgct gaccggcggg cattcttcct gccgtcctgg cggctttttc ggtccgctgc
     5641 ttcagagtgg ccaactgcac aaacggatac ggaggcgcaa gcgaaaaatc cccccgcgtc
     5701 agcgccagtg cttcattaat gcgcgcaccg ttgttccaca gtgtggccag cagcattttg
     5761 cggcgcagat ccggcacgta atggagcagg gcgctcactt caggggccag caggtatttt
     5821 ggcagttcat catgaaccat cgacatctgg cggagtgcca gcgctgccgg ataatcgatg
     5881 gcaacaggaa gctgagcagc atcccgggca tggtggatcg gtgccggcag gtgctgcata
     5941 tcagtgttca ctccacgggg aaacgtcggg caggttttcc acaacacggt actttcccgg
     6001 ataacggagc tggttacctc cacgcgtcca gacggtgaca acaaaacccc ctccctggtt
     6061 gttttcgggg agcaggcgat cgcactgctc actcagcatg tggatcacaa acggcgcatc
     6121 gctgccgtct tcaaatacca gttccagcgc atcgcggcca tgcagtgctc ccttactgat
     6181 gatgacgtac tcagcccccc gcatctccct gagtaaatgt tttgccgcat ccgggacaag
     6241 cagcctggct gcgccggcat tccagctgag gtagacatat ccggcctgcg cctgtacaga
     6301 ctgccagtaa tccgttccgg cgacagcctg accttcattc tcaataaaaa acatgggtga
     6361 ctcctttttt cttttccggg cagggacggt catgcgataa cgtcatccat catcactgcc
     6421 cttaccgtca cgccgtctga taacatcatg ccgttatcat catcaccatg ataacaagca
     6481 tccatataac cacgccgtta aattaccgga gagccgtttc cgtcagatcc gactttccgg
     6541 aatatcacaa ctttccggaa gtgtaccacg ctgagaaaat catcatatcg ctgttttccg
     6601 cccctgcgcg gcgcatcaca ggtcagggag atgacacaac atatagtgtc atcagcagct
     6661 gaattcagca ctacatgttg tgctgacggg agatggcgct gtgcggaggg agaggaagat
     6721 ccgtaacagc cggaaaccgg atctgtgcgg cagagaacgg gcaggagata tgataattaa
     6781 gcagcacaca taaaatcata tctgatatga ttttcaccat taaatataaa accatacctg
     6841 atattatcat aaatcaataa cacacaaatc atgcaggatg tgatttttac ctttaagaac
     6901 attatcagat ggagcgtgca ggatggatga agaaagggtg ttttcactca gctatgaaca
     6961 actaacccgg tttacggaac gacgcatccg ggaatgcaat ctggacagcc agggcgcgat
     7021 ttatctctgc gagtcagcca aagccggagc cgtgctgatc ttctggcatg aactggccat
     7081 aaatggctat gcctcaatga atgccataaa gagacaggag ctcattgacg ccgactttca
     7141 gcgcctcaga aagctcatct ggccggagga tgactggaaa tgactcagag ccgccgaccg
     7201 tcgccactgc agcggagagt gctgattgtg ctggctgccc ttgatgaaaa acggccagga
     7261 cccgtgctga cgcgggacat agaacgggtg ctggagcgaa gcggggaagc cccggtctac
     7321 gggccaaacc tgcgcgcctc ctgccgtcgt ctggaggatg ccggctggct gcgtaccctg
     7381 cgcgctccga acctgcagct ggcagtggag ctgacggacg ccggccgggc cgtggcacag
     7441 cctctgctgc tgacagaaca ggaccgcctc cgggcagaac agcgtgcggc agaagtcgtc
     7501 gtactgccgc tggtcccggc agcaggtctg cccgcagatg gcacttctgc cactgatctg
     7561 gaggtggagc tgaacggcat aacgtatcag gcctgccgtg gggattttgt ggtgcgtctc
     7621 gacggcagca cctgtctgca gttatggaat aaagaaggca gagtggtacg ccgggagggc
     7681 gatccgctgg aagtggcgca gtggctgcag gcctgtcatg atgccggaat agaagttcgt
     7741 gtacaggtta atgaaagcgt cactccgtaa aaacatgcca gcacggttta cacatcaccc
     7801 catggtggca gccggttaac tttcctgcgc caccagtgga aagaggccgt gagcagtccg
     7861 aacaaaaaac cacccgtgat acttataatt atggcctcac ctgccaccat tcctgacggc
     7921 ccccggtaga taaaccacat tgcacatccc caggaaatgc cccataagct ccccatcaac
     7981 agcgtgacct gccagaacgg cataaagggt aatggcggaa gccggatacc cagccgccag
     8041 agaatacaca gaagaggagg ggcataattg ctccgccaca tctttttgct gtccatcagg
     8101 gcaatggccc gggctttttt ctgctcaaag gtcacaggag cacctccgtt ccatgatgta
     8161 aacgtgacac agcttattgt tattgattat
//
LOCUS       DAJOWB010000175         8045 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJOWB010000175 DAJOWB010000000
VERSION     DAJOWB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.175"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            171..530
                     /locus_tag="OHO85_004383"
     CDS             171..530
                     /locus_tag="OHO85_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCQ2673650.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(650..829)
                     /locus_tag="OHO85_004384"
     CDS             complement(650..829)
                     /locus_tag="OHO85_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCQ2673651.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            903..2264
                     /gene="pabB"
                     /locus_tag="OHO85_004385"
     CDS             903..2264
                     /gene="pabB"
                     /locus_tag="OHO85_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCQ2673652.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2268..2846
                     /locus_tag="OHO85_004386"
     CDS             2268..2846
                     /locus_tag="OHO85_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCQ2673653.1"
                     /translation="MEYRSLTLDYFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3030..4394
                     /gene="sdaA"
                     /locus_tag="OHO85_004387"
     CDS             3030..4394
                     /gene="sdaA"
                     /locus_tag="OHO85_004387"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCQ2673654.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMCFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4525..6123
                     /locus_tag="OHO85_004388"
     CDS             4525..6123
                     /locus_tag="OHO85_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCQ2673655.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6127..7689)
                     /gene="yoaE"
                     /locus_tag="OHO85_004389"
     CDS             complement(6127..7689)
                     /gene="yoaE"
                     /locus_tag="OHO85_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCQ2673656.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PIPEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHS
                     LFPVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRR
                     ARGSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHA
                     LQQALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVR
                     IVKEQPAHDEDE"
BASE COUNT         1996 a         2066 c         2008 g         1975 t
ORIGIN      
        1 actagcaact ctggttttct ttcttagctg aataatctgg ttatagcgtg aggactgacc
       61 gttaaatttc tccatcttac tggataacac accatgagct gctaccttta aaatacctga
      121 atgtcaacag gttaactcgt gccatatcgt tataatccct ttgatattag atgcaaatta
      181 aggtcatata tagtcttatc gataacatgg ttaattttaa ggataagaat atgcctgctg
      241 taatagataa agccctggat ttcattggtg ccatggatgt atcagcgcca acaccaagtt
      301 cgatgaatga aagcacggcg aagggaatct ttaaatattt aaaagaactg ggagtacccg
      361 cgagtgccgc tgatattacg gcgcgagctg accaggaagg ctggaaccct gggttcacgg
      421 aaaaaatggt tggatgggca aaaaaaatgg agacaggtga acgctctgtg attaaaaatc
      481 ctgaatactt ttcaacatat atgcaggaag aactaaaagc actggtctga gttaaattta
      541 tatcagcata aatgggtcag gacgctttta atcacatata aaaagcgtcc tttttctcac
      601 aatcaaatga tcatcggctt ctataacgaa atcgatacgg attagtgtgt tactcgtctt
      661 catcctcaaa acgcgccacg attcgctcgc cggagtggtt ggcacgcagc tcttctgcca
      721 ccagcgcgat tgcctggccg ctgctcatcc cttgtgccat cagttcctgg atccgctcga
      781 cagctttttg ctgctgttca tgggtgagtg aaggtaaacc tgcaaacatt gttaactcct
      841 gctaaattgt tggcgctaat tatttcatgc tacccggcac atagccagta gagtcaggac
      901 tgatgaagac gttatctccc gctgtgatta ctttaccctg gcgtcaggac gccgctgaat
      961 tttatttctc ccgcttaagc cacctgccgt gggcgatgct tttacactcc ggctatgccg
     1021 atcatccgta tagccgcttt gatattgtgg tcgccgatcc gatttgcact ttaaccactt
     1081 tcggtaaaga aaccgttgtt agtgaaagcg aaaaacgcac aacgaccact gatgacccgc
     1141 tacaggtgct ccagcaggtg ctggatcgcg cagacattcg cccaacgcat aacgaagatt
     1201 tgccatttca gggcggcgca ctggggttgt ttggctacga tctgggccgc cgttttgagt
     1261 cactgccaga aattgcggaa caagatatcg ttctgccgga tatggcagtg ggtatctacg
     1321 attgggcgct cattgtcgac caccagcgtc atacagtttc tttgctgagt cataatgatg
     1381 tcaatgcccg tcgggcctgg ctggaaagcc agcaattctc gccgcaggaa gatttcacgc
     1441 tcacttccga ctggcaatcc aatatgaccc gcgagcagta cggcgaaaaa tttcgccagg
     1501 tacaggaata tctgcacagc ggtgattgct atcaggtgaa tctcgcccag cgttttcatg
     1561 cgacctattc tggcgatgaa tggcaggcat tccttcagct taatcaggcc aaccgcgcgc
     1621 catttagcgc ttttttacgt cttgaacagg gtgcaatttt aagcctttcg ccagagcggt
     1681 ttattctttg tgataatagt gaaatccaga cccgcccgat taaaggcacg ctaccacgcc
     1741 tgcccgatcc tcaggaagat agcaaacaag cagaaaaact ggcgaactca gcgaaagatc
     1801 gtgccgaaaa tctgatgatt gtcgatttaa tgcgtaatga tatcggtcgt gttgccgtag
     1861 ccggttcggt aaaagtacca gagctgttcg tggtggaacc cttccctgcc gtgcatcatc
     1921 tggtcagcac tataacggcg cgactaccag aacagttaca cgccagcgat ctgctgcgcg
     1981 cagcttttcc tggtggctca ataaccgggg ctccgaaagt acgggctatg gaaattatcg
     2041 acgaactgga accgcagcga cgtaatgcct ggtgcggcag cattggctat ttgagctttt
     2101 gcggcaacat ggataccagc attactatcc gcacgctgac tgccattaac ggacaaatat
     2161 actgctctgc gggcggtgga attgtcgccg atagccagga agaagcggaa tatcaggaaa
     2221 cttttgataa agttaataag atattacgcc aactggagaa gtaagacgtg gaataccgta
     2281 gcctgacgct tgattatttt ttatctcgct ttcaactttt gcgcccgcaa attaaccggg
     2341 aaaccctaaa tcatcgtcag gctgccgtgt taatccccat cgtccgtcga ccgcaaccgg
     2401 ggttgttgct gactcagcgt tcaattcatc tgcgtaaaca cgctggacaa gtggcattcc
     2461 ctggaggtgc agtcgatgac acggacgcat cagttatcgc cgccgcgctg cgcgaagctg
     2521 aagaagaggt cgctataccg ccttccgccg ttgaagttat cggcgtgctg ccgcccgtcg
     2581 atagcgtaac tggctatcag gtaaccccag tggtcggcat tatcccgccc gatctgccgt
     2641 atcgcgccag tgaagatgaa gtctcggcgg tgtttgaaat gccgctcgcc caggcattac
     2701 atctgggtcg ttatcaccct ttagatatct accgccgtgg cgattcacat cgggtatggc
     2761 tgtcctggta cgaacagtat tttgtatggg gaatgaccgc aggcataatt cgtgagctgg
     2821 cgctgcaaat tggtgtgaaa ccctgactat acttatcttt acatctacaa aacactactt
     2881 gagacaatca tcgcaatatt agttaaatcg cggtttttga ttagtttaat tcatgtgaat
     2941 agttaagcca gtcgccgcgt tccctcttac actatgcgct gttattagtt cgttactgga
     3001 agtccagtca ccttgtcagg agtattatcg tgattagtct attcgacatg tttaaggtgg
     3061 ggattggtcc ctcatcttcc cataccgtag ggcctatgaa ggcgggtaaa cagttcgtcg
     3121 atgatctggt cgaaaaaggc ttactggata gcgttactcg cgttgccgtg gacgtttatg
     3181 gttcactgtc gctgacgggt aaaggccacc acaccgatat cgccattatt atgggtcttg
     3241 caggtaacga acctgccacc gtggatatcg acagtattcc cggttttatt cgcgacgtag
     3301 aagagcgcga acgtctgctg ctggcacagg gacggcatga agtggatttc ccgcgcgaca
     3361 acgggatgtg ttttcataac ggcaacctgc cgctgcatga aaacggtatg caaatccacg
     3421 cctataacgg cgatgaagtc gtctacagca aaacttatta ttccatcggc ggcggtttta
     3481 tcgtcgatga agaacacttt ggtcaggatg ctgccaacga agtgagcgtg ccgtatccgt
     3541 tcaaatctgc caccgaactg ctcgcgtact gtaatgaaac cggctattcg ctgtctggtc
     3601 tcgctatgca gaacgaactg gcgctgcaca gcaagaaaga gatcgacgag tatttcgcgc
     3661 atgtctggca aaccatgcag gcatgtatcg atcgcgggat gaacaccgaa ggtgtactgc
     3721 caggcccgct gcgcgtgcca cgtcgtgcgt ctgccctgcg ccggatgctg gtttccagcg
     3781 ataaactgtc taacgatccg atgaatgtca ttgactgggt aaacatgttt gcgctggcag
     3841 ttaacgaaga aaacgccgcc ggtggtcgtg tggtaactgc gccaaccaac ggtgcctgcg
     3901 gtatcgttcc ggcagtgctg gcttactatg accactttat tgaatcggtc agcccggaca
     3961 tctatacccg ttactttatg gcagcgggcg cgattggtgc attgtataaa atgaacgcct
     4021 ctatttccgg tgcggaagtt ggttgccagg gcgaagtggg tgttgcctgt tcaatggctg
     4081 ctgcgggtct tgcagaactg ctgggcggta gcccggaaca ggtttgcgtg gcggcggaaa
     4141 ttggcatgga acacaacctt ggtttaacct gcgacccggt tgcagggcag gttcaggtgc
     4201 cgtgcattga gcgtaatgcc attgcctctg tgaaggcgat taacgccgca cggatggctc
     4261 tgcgccgcac cagtgcaccg cgcgtctcgc tggataaggt catcgaaacg atgtacgaaa
     4321 ccggtaagga catgaacgcc aaataccgcg aaacctcacg cggtggtctg gcaatcaaag
     4381 tccagtgtga ctaatacttc ttactcgccc atctgcaacg gatgggcgaa tttatacccg
     4441 ctttctcgtc tgctgtaata ttccccacta cacttccact gttgcgtcag gcgtttgtcg
     4501 ccatacgctt acagggtggc ccgcatgcaa aaagcacaac ggatcattaa aacctatcgc
     4561 cgtaatcgaa tgattgtttg tacgatttgc gcactcgtta cgctcgcttc gaccctgagc
     4621 gtgcgattta tttcacagcg taacttaaat caacaacggg tagtacaatt cgccaatcac
     4681 gctgtagagg aattagataa agtactgctt cccctacagg caggtagcga agtcttgctt
     4741 ccgctgattg gtctgccctg ctctgtcgcc catttgccat tacgtaaaca ggcggcaaaa
     4801 ctccaaactg tgcgatccat tggcctggtg caagacggca cactttattg ctccagcatt
     4861 tttggttatc gcaatgtgcc cgtcgtggac attctggctg aacttcctgc accgcaacca
     4921 cttttacgcc tgacgatcga ccgtgccctg attaaaggca gtccggtttt gattcaatgg
     4981 acgcctgcag cgggcagtag caatgctggg gtcatggaga tgattaacat cgacttactg
     5041 acggcaatgc tccttgagcc acaactgccg caaatcagta gcgccagcct gacggtggac
     5101 aaacggcatt tgctctatgg taatgggctg gtagattccc ttccgcaacc tgaagacaat
     5161 gaaaactatc aggtttcttc gcaacgcttt ccttttacca ttaacgttaa tggtccgggg
     5221 gctacggcgc tggcatggca ctatcttcca acacaattac cgctggcggt gctgctaagt
     5281 ttactggtgg gctacatcgc ctggctggcg accgcttacc ggatgagctt ttcccgcgaa
     5341 atcaatctgg gcctggcgca acatgagttc gaattgttct gtcagccttt gcttaatgcg
     5401 cgcagccagc aatgtattgg tgtagagatt ttgctgcgct ggaacaatcc gcgtcagggc
     5461 tggatttcac cggatgtgtt tattcctatc gcggaagaac atcatttaat tgtgccactg
     5521 acccgctatg tgatggcaga aaccattcgt cagcgccatg ttttcccgat gagtagtcag
     5581 tttcatgttg gcattaacgt cgcacccagc cattttcgcc gtggtgtgct gataaaagat
     5641 ctcaatcagt actggtttag cgctcacccg attcagcaac tgatcctcga aatcaccgaa
     5701 cgcgatgcct tactggatgt tgattatcgg attgcccgcg agctacatcg taaaaacgtc
     5761 aaactggcga ttgatgactt cggcaccggc aacagttcat tttcctggct tgaaacatta
     5821 cgtcctgacg tgctgaaaat tgataagtca tttaccgcag ctataggttc tgacgcggtt
     5881 aactcgacgg tgaccgatat catcatcgcg ctggggcaaa gactgaatat tgaactggtg
     5941 gcggagggcg tggaaacgca agaacaggcg aagtatttgc gccgtcatgg cgtgcatatt
     6001 ttgcaagggt atttgtacgc acagccgatg ccgctacgtg attttcccaa atggctggcg
     6061 ggcagccaac cgccgcccgc ccggcataat ggacatatca cgcccattat gccgttacgt
     6121 taatgcttac tcatcttcat cgtgcgccgg ttgctcttta acaatgcgaa ccagatcaac
     6181 acgataatca ttggcttcaa tgatggtgat atgcagtggc cctacatcaa tcacatcgcc
     6241 cacacgggga atgtgaccat ttgccgagat cacgaggccc gcgaccgtcg cgatatcatc
     6301 gtcatcggca aggtgctcaa catcaagcgc ctgctgcaag gcatgcaaat ctgtaccgcc
     6361 ttttaccagc cagccgtcac catcagtaat gatttccggc gtttcgtcag cgtccgggaa
     6421 ttcacccgca atggcttcca gcacatccag cggcgtgacc agaccttgta ccacaccaaa
     6481 ctcgttggtc acgataacaa agctcccgcg agcacgacgc agcacgccca acaggttgat
     6541 cggatcgagg gtttccggga cgataatcgc cggagacgcc gaagcaatcg ccgccacatc
     6601 aacgccctct tccagcgcca ccagcagttc tttagcacgt acaataccga tgatttcatc
     6661 cagttcaccg cgacataccg ggaacagact gtgcggtgaa gagagcagtt gctcgcggat
     6721 ttcatcgacc ccgagattag cgtcaaccca gcttatttca ccgcgcggcg tcatgatccc
     6781 gcgcagagaa cgcgacgcca gcgtcagtac gccgttaatc atgtaacgtt cttcttcggc
     6841 aaatgcacct tccgggatcg ggatcggcat cggcatcggg ttatcggcat cgtgctgaac
     6901 attggcctga cgtttcccgc ccatcaaacg caggatggca tcggcagtac gcgctcgcag
     6961 cggcaaagtc gactggtggc gaataaagtt gcgacgcgca atctggttaa acacttcgat
     7021 gatgatcgag aagccaatcg cggcatacag gtaacctttc ggaatgtgga aaccgaaacc
     7081 ttctgccacc agactcagac caatcattaa caggaagctc agacagagca ccaccaccgt
     7141 ggggtgctgg ttaacgaatc gcgtcagcgg tttggatgcc agcaacataa ccgccatcgc
     7201 aatcactacc gccgccatca tcaccggcag atggttaacc atccctactg cagtaattac
     7261 cgcatccaac gagaagacgg cgtcaaggat gacaatctgt gtgacgacca cccagaaact
     7321 ggcgtagcct ttaccgtggc cggaatcatg atcgcggttt tccagccgtt catgcagttc
     7381 ggttgttgct ttgaacagca agaatatccc cccgaacaac ataatcaggt cgcgtccgga
     7441 gaaggagaaa tccatgacgg taaatagcgg tttggtcagc gtgaccatcc atgaaatcag
     7501 cgacagcagc cccagacgca taatcagcgc cagtgataac cccagcaaac gcgctttatc
     7561 gcgttgtttt ggcggcagtt tgtcagcaag aatggcgatg aagaccaggt tatcgatacc
     7621 cagcacaatt tcgagaacaa caagcgtgag tagccccgcc caaattgagg ggtccattaa
     7681 gaattccatg acaagctcct gcttaaggaa tagctattcg acgccagaaa taatgcaggc
     7741 gtaacgacaa aatgcaaacg aaaggtgcgg catagagagc cagaaaggta ggcgttaaaa
     7801 ggcctgatgc tgaaatgacg tcggtgacga tccatactgc gggctactgc cctatactcc
     7861 atggttgtta aacgggagtt aaacatatca gagacgcctc tgatttggca aagatttacc
     7921 ttcctttgca aacgaatgtg acaaggatat tttacctttc gaaatttctg ctaatcgaaa
     7981 gttaaattac ggatcttcat cacataaaat aattttttcg atatctaaaa taaatcgcga
     8041 aacgc
//
LOCUS       DAJOWB010000176         7959 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJOWB010000176 DAJOWB010000000
VERSION     DAJOWB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.176"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..834
                     /locus_tag="OHO85_004390"
     CDS             <1..834
                     /locus_tag="OHO85_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HCQ2673657.1"
                     /translation="LRHRDIIIAKIDLFIQRITHNLWHARKKNVFLIEQITNLKVWVN
                     KYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNKDATPIERCTFDVETKNGL
                     PSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHDDNSLKLFASLLISLSYSS
                     AYTDLAGAEKVNINEYNENYLTAQFEELSQRDMKKYLGEMKHLADRGGMKFDDYLDKM
                     SLLVNEGKLEPDILSKMRDAAPKLIDFAKSFDPNSKEKIKILTDTSNLIYDLFGVKSA
                     K"
     gene            1198..2235
                     /gene="dusA"
                     /locus_tag="OHO85_004391"
     CDS             1198..2235
                     /gene="dusA"
                     /locus_tag="OHO85_004391"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCQ2673658.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2369..2611
                     /gene="pspG"
                     /locus_tag="OHO85_004392"
     CDS             2369..2611
                     /gene="pspG"
                     /locus_tag="OHO85_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCQ2673659.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2777..3760)
                     /locus_tag="OHO85_004393"
     CDS             complement(2777..3760)
                     /locus_tag="OHO85_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCQ2673660.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3843..5258
                     /gene="dnaB"
                     /locus_tag="OHO85_004394"
     CDS             3843..5258
                     /gene="dnaB"
                     /locus_tag="OHO85_004394"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCQ2673661.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5311..6390
                     /gene="alr"
                     /locus_tag="OHO85_004395"
     CDS             5311..6390
                     /gene="alr"
                     /locus_tag="OHO85_004395"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCQ2673662.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6643..7836
                     /gene="tyrB"
                     /locus_tag="OHO85_004396"
     CDS             6643..7836
                     /gene="tyrB"
                     /locus_tag="OHO85_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCQ2673663.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLKQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1953 a         1886 c         2063 g         2057 t
ORIGIN      
        1 ctgcgtcatc gggatataat tattgcaaaa atagatttgt ttattcaaag aatcactcat
       61 aatttatggc atgcacgtaa aaaaaatgtg tttttaattg agcaaataac taatctcaaa
      121 gtgtgggtca ataagtatat tgacgattgc actgatgagg atttaaacga tcgtgacttt
      181 attgcatcag ttgttgaccg ggctattttt catttcgcga ttaatagtat atgtaatcct
      241 ggggataata aagatgcgac tcccattgaa cgatgtactt ttgatgtaga aactaagaat
      301 ggccttccct ccacggttca gctattttat gaggaatcta aggataatga acctttagcg
      361 aatatacatt ttcaagcaat aggttctggt tttttaacgt ttgttaatgc ctgccaggaa
      421 catgatgaca acagcttaaa attatttgct tcgctgttaa tttcactttc atattctagt
      481 gcctacacag atttagccgg agcagaaaaa gtgaatatta atgaatataa tgagaactat
      541 ctgacagctc agtttgaaga attatctcaa cgtgatatga agaagtacct gggagagatg
      601 aagcatctgg cggaccgggg ggggatgaaa tttgatgact atctggataa aatgtcactt
      661 ctggtgaatg aaggaaagct cgaacctgat attttaagca aaatgcgaga tgctgcaccg
      721 aaattaattg actttgctaa gtcgtttgac ccaaactcaa aggaaaagat taaaatactt
      781 acagatactt ctaatttaat ttatgattta ttcggggtta aatcggcgaa ataatatgtg
      841 aagttcttcg atggtatgga aggcattaca taaaagaacc taatacttat tgggttcttt
      901 tttcttctat cagtgccatt agcaggaaga gatatcaccg gagtttaatg tgtgattttt
      961 tatttatcgt cgaacctgga ttgtttatca ttgtccttaa caaggctaac ggctaataag
     1021 attatttcca tcacttcgtg agagcttcat gccttgattt gatctcaatt ttcttttgca
     1081 atgagacagg cgcttcctgt tgttatggta tagtaccccg ctattgagcc tcctgaacag
     1141 tgatgctgaa taacataacc caatgatata tcgataaaat aatctctaca tttgaaaatg
     1201 cacggtaatt ctgaaatgca aaaaatcaac caaaccagcg caatgcctga aaaaactgac
     1261 gttcactgga gtggtcggtt tagcgttgca ccaatgctcg actggacgga cagacattgc
     1321 cgctatttct tgcgtctgct ttcccgcaat acgttgctgt ataccgaaat ggtgaccaca
     1381 ggggcgatta ttcacggtaa aggtgattac ctggcgtaca gtgaagaaga acatccggta
     1441 gcgttgcaac tcggcggtag cgatccggcg gcgctggcac agtgtgcgaa gctggcagaa
     1501 gcgcgtggat atgatgagat caacctgaat gtcggctgcc cgtctgaccg ggtgcagaac
     1561 ggcatgtttg gtgcgtgtct gatgggtaat gcgcagctgg ttgccgactg tgtgaaagcg
     1621 atgcgcgatg tggtgtcgat tccagtgacg gtgaaaacgc gtattggcat cgatgaccag
     1681 gacagctatg aatttctctg cgatttcatc aacactgttt ccggcaaagg cgagtgtgag
     1741 atgtttatca tccacgcacg taaagcctgg ctttcggggt taagcccgaa agaaaaccgt
     1801 gaaatcccgc cgctcgatta tccgcgtgtg tatcaactga agcgtgactt tccgcatctg
     1861 acaatgtcga ttaacggtgg tatcaagtcg ctggaagagg ccaaagcaca cctgcaacat
     1921 atggatggcg tgatggtcgg gcgcgaggcg tatcagaatc cgggtattct ggcggcagta
     1981 gaccgggaga tctttggttc ctcggatacc gatgccgatc cggtggcggt agtgcgcgcc
     2041 atgtatccgt acattgagcg tgaactcagc caggggacgt atctcggcca tattacccgg
     2101 catatgttgg gcttgttcca gggtattcct ggcgcgcggc agtggcggcg ttatttaagt
     2161 gaaaatgccc ataaagcggg tgcagacatt aatgtgctgg aacacgcgct caaactggtg
     2221 gcggataagc gttaactttt caccaaaaag tagtcaaatt caccacgccc tgcgcaccgt
     2281 cgcggggcgt tttgctgtta aatcaataga ttatttttgg catgattctt gtaatgccag
     2341 caagagattt catatttggg agagcatcat gctggaacta ctttttgtga ttggcttttt
     2401 tgtcatgctg atggtcaccg gcgtttcgtt gctgggcatt atcgccgcgc tggttgtggc
     2461 gacggccatt atgttcctcg gcggtatgct ggcattgatg attaagttgc tgccgtggtt
     2521 actactggcg attgcggtgg tgtgggttat taaggcgatt aaagcaccaa aagtgccgaa
     2581 atatcagcgt tatgaccgct ggcgttacta agggattgtg cggatgatca caacctaagg
     2641 ttttatcctt agaacaaaat aggaattgat aatcaaatct gtcactattg cgcctctaac
     2701 agattcatcg tgctgtaccc tacatacagc cgaactataa aaagaaaggg cttcccaggt
     2761 ggaagcccta tttcttttat ggaatcaaca ggctggaacc ttgcgtcgcc cggctttcca
     2821 gaatctcatg cgcacgctgc gcatccttca gcggatattt ctgctgctcg gcgacatcga
     2881 ccttaatcac accgctggca atcaaagaga acagttcatt actggcctcg gttaattcct
     2941 cccgcgtggt gatatagcct tgcagggaag ggcgtgtcac atacaacgag cctttttgat
     3001 tgagaatgcc taagttcaca ccggtaaccg cacctgatga gttgccaaaa ctgaccatta
     3061 agccgcggcg ttgcaggcaa tccagcgacc gttcccaggt gtctctgccc acggaatcgt
     3121 acaccacgcg cactttctta ccgccggtga tctcttttaa ccgctcgacc agatcctctt
     3181 cacgatagtt aataacctgc cacgcgcccg cttttagcgc gctctgcgct ttttgcgcgg
     3241 ttcctacggt gccgataagt ttcgcgccca gggcttttgc ccactggcag gcaatcaagc
     3301 caacgccgcc agctgctgcg tggaacagga actgctcatc gggtttaatt tcataggttt
     3361 tgcgcagcag ataataaacc gttaagcctt tcaggaaaga tgctgcagct tgctcaaaag
     3421 aaattgccgc aggcagaatc gccgctttat ccgcattaat gttatgcaca gagctgtaag
     3481 cgcctaacgc cgactgcgca tagactacac gatcgcctgc cttaatatgc tttacaccac
     3541 tgccgacttt actcacgatg cctgccgctt cggtacctaa tccgctgggt agtgatggcg
     3601 gcgggtaaag gccgctgcgg atatacgtgt cgataaaatt gatgccgatg gctttatttt
     3661 cgacctggat ttcattctct gccggatcgg caggagtgaa ctctacggct tgaagtactt
     3721 ccgggccacc gtgcttgtga aattcaattc gtgttgccat gtgtcctcca gaacgtatcg
     3781 tcagggtctg cttcatatgg taaagtttcg acccattctt tatctcggta actccattca
     3841 ctatggcagg aaataaaccc ttcaacaaac agcaggctga accccgcgaa cgcgatccac
     3901 aagttgccgg gctgaaagtg cctccgcact cgatcgaagc ggagcagtcg gtgttgggcg
     3961 gtttaatgct ggataacgaa cgctgggacg atgtagccga gcgtgtggtg gcagacgatt
     4021 tttacacccg cccacaccgc catatcttta ctgaaatggc gcgtttgcag gaaagcggta
     4081 gtcctatcga tctgattacc cttgcggaat cgctggaacg ccaggggcaa ctcgatagcg
     4141 tcggtggttt tgcttatctg gcagagctgt caaaaaatac gccaagtgcg gcgaacatca
     4201 gtgcttatgc tgacatcgtg cgtgaacgtg ccgttgtccg tgagatgatc tcggttgcga
     4261 atgagattgc tgaagccggt tttgatccgc aggggcgtac cagcgaagat ctgctggacc
     4321 ttgctgaatc ccgcgtcttt aaaattgccg aaagtcgtgc aaacaaagac gaagggccga
     4381 agaacatcgc cgatgtgctc gacgcaaccg tggcgcgtat tgagcagttg tttcagcagc
     4441 cacacgatgg cgttaccgga gtaaacaccg gttatgacga tctcaacaaa aaaaccgctg
     4501 gcttgcagcc gtcggatttg atcatcgtcg ccgcgcgtcc gtcgatgggt aaaacaacat
     4561 ttgcgatgaa cctcgtcgaa aacgcggcga tgttgcagga taaaccagta cttatcttct
     4621 cgctggagat gccttcagaa cagattatga tgcgttctct ggcgtcgctg tcgcgcgttg
     4681 accagactaa aatccgtacc gggcagctcg atgatgaaga ctgggcgcgc atttccggca
     4741 ccatgggtat tttgctcgaa aaacgcaata tctatatcga tgactcctcc ggcctgacgc
     4801 caacggaagt gcgttcccgc gcacgccgta ttgcccgtga acacggcggc atcgggctta
     4861 tcatgatcga ctacctgcaa ctgatgcgcg taccggcgct ttccgataac cgtacgctgg
     4921 aaattgcaga aatctcccgc tcgctgaaag cactggcgaa agaactgaac gtgccggtgg
     4981 tggcgctgtc ccagttgaac cgttctctgg aacaacgtgc cgacaaacgc ccggtcaact
     5041 ccgacctgcg tgaatctggc tctatcgagc aggatgcgga cttgatcatg tttatctatc
     5101 gtgatgaggt gtatcacgaa aacagtgatt taaaaggcat cgcggaaatt attatcggta
     5161 aacaacgtaa cggcccaatc gggacggtac gcctgacctt taacggtcaa tggtcgcgct
     5221 tcgacaacta tgcggggccg cagtacgacg acgaataata attattttat gaattaggta
     5281 attaaagcaa acacttatca aggaacacaa atgcaagcgg caactgttgt gattaaccgc
     5341 cgcgctctgc gacacaacct gcaacgtctt cgtgaactgg cccctgccag taaaatggtt
     5401 gcggtggtga aagcgaacgc ttatggtcac ggtcttcttg agaccgcgcg aacgctcccc
     5461 gatgctgacg cctttggcgt agcccgtctc gaagaagctc tgcgactgcg tgcgggggga
     5521 atcaccaaac ctgtactgtt actcgaaggc ttttttgatg ccagagatct gccgacgatt
     5581 tctgcgcaac attttcatac cgccgtgcat aacgaagaac agctggctgc gctggaagag
     5641 gctagcctgg acgagccggt taccgtctgg atgaaactcg ataccggtat gcaccgtctg
     5701 ggcgtaaggc cggaacaggc tgaggcgttt tatcatcgcc tgacccagtg caaaaacgtt
     5761 cgtcagccgg tgaatatcgt cagccatttt gcgcgcgcgg atgaaccaaa atgtggcgca
     5821 accgagaaac aactcgctat ctttaatacc ttttgcgaag gcaaacctgg tcaacgttcc
     5881 attgccgcat cgggtggcat tctgctgtgg ccacagtcgc attttgactg ggtgcgcccg
     5941 ggcatcattc tttatggcgt ctcgccgctg gaagatcgct ccaccggtgc cgattttggc
     6001 tgtcagccag tgatgtcact aacctccagc ctgattgccg tgcgtgagca taaagccgga
     6061 gaacctgttg gttatggtgg aacctgggta agcgaacgtg atacccgtct tggcgtagtc
     6121 gcgatgggct atggcgatgg ttatccgcgc gccgcgccgt ccggtacgcc agtgctggtg
     6181 aacggtcgcg aagtaccgat tgtcgggcgc gtggcgatgg atatgatctg cgtagactta
     6241 ggtccacaag cgcaggacaa agccggggat ccggtcattt tatggggcga aggtttgccc
     6301 gtagaacgta tcgctgaaat gacgaaagta agcgcttacg aacttattac gcgcctgact
     6361 tcaagggtcg cgatgaaata cgtggattaa tcgttctgta atatttgatt gtctgtgccg
     6421 gatgcggcgt gaatgcctta tccggccaat aaaatcctaa aaattcaata agttgatgtt
     6481 ctttcatgct cttataaagg tcgtgcctct ggcggatgta cgtttgtcat gagtctcact
     6541 ctgttgctaa ttgccgttcg ctcctgaaca tccactcgat cttcgccttc ttccggttta
     6601 ttgtgtttta accacctgcc cgtaaacctg gagaaccatc gcgtgtttca aaaagttgac
     6661 gcctatgctg gcgacccgat tcttacgctt atggagcgtt ttaaagaaga ccctcgcagc
     6721 gacaaagtga atttaagtat cggtctgtac tacaacgaag acggaattat tccacaactg
     6781 aaagccgtgg cggaggcgga agcgcgcctg aatgcgcagc ctcatggcgc ttcgctttat
     6841 ttaccgatgg aagggcttaa cagctatcgc catgccattg cgccgctgct gtttggtgcg
     6901 gaccatccgg tactgaaaca acagcgcgta gcaaccattc aaacccttgg cggctccggg
     6961 gcattgaaag tgggcgcgga tttcctgaaa cgctacttcc cggaatcagg cgtctgggtc
     7021 agcgatccta cctgggaaaa ccacgtagca atattcgccg gggctggatt cgaagtgagt
     7081 acttacccct ggtatgacga agcgactaac ggcgtgcgct ttaatgacct gttggcgacg
     7141 ctgaaaacat tacctgcccg cagtattgtg ttgctgcatc catgttgcca caacccaacg
     7201 ggtgccgatc tcactaatga tcagtgggat gcggtgattg aaattctcaa agcccgcgag
     7261 cttattccat tcctcgatat tgcctatcaa ggatttggtg ccggtatgga agaggatgcc
     7321 tacgctattc gcgccattgc cagcgctgga ttacccgctc tggtgagcaa ttcgttctcg
     7381 aaaattttct ccctttacgg cgagcgcgtc ggcggacttt ctgttctgtg tgaagatgcc
     7441 gaagccgctg gccgcgtact ggggcaattg aaagcaacag ttcgccgcaa ctactccagc
     7501 ccgccgaatt ttggtgcgca ggtggtggct gcagtgctga atgacgaggc attgaaagcc
     7561 agctggctgg cggaagtaga agagatgcgt actcgcattc tggcaatgcg tcaggaattg
     7621 gtgaaggtat taagcacaga gatgccagaa cgcaatttcg attatctgct taatcagcgc
     7681 ggcatgttca gttataccgg tttaagtgcc gctcaggttg accgactacg tgaagaattt
     7741 ggtgtctatc tcatcgccag cggtcgcatg tgtgtcgccg ggttaaatac ggcaaatgta
     7801 caacgtgtgg caaaggcgtt tgctgcggtg atgtaatgca ggaaagcagg ctggagctac
     7861 ccagcctgca gtgaaattaa actgtcgtcg ctttcactct ttctttatag atgatttttt
     7921 tgatgccatc gttctacgtg agagataata aacgttatt
//
LOCUS       DAJOWB010000177         7846 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJOWB010000177 DAJOWB010000000
VERSION     DAJOWB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.177"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            251..2359
                     /locus_tag="OHO85_004397"
     CDS             251..2359
                     /locus_tag="OHO85_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCQ2673664.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIALTGHPQANLNREWQVVASELHGEQPQAVPGR
                     SGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
     gene            2427..6647
                     /locus_tag="OHO85_004398"
     CDS             2427..6647
                     /locus_tag="OHO85_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCQ2673665.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDI
                     RLQLRDDGLILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRELTGLADRFGR
                     TLTYRREAAGDLTGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     AFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPAAPLVRYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQDRIT
                     VTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSRSGE
                     TVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTA
                     VHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQYDAW
                     GKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQRYH
                     YDLTGKLTQSEDEGLVTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGH
                     RVAVHYGYDDKGRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEW
                     LTYGSGYLAGMKLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQS
                     QHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYAT
                     DPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDD
                     ERTHHYHYDSQHRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLS
                     RKPEVTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEK
                     LQQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRVWLAQCGLTVEQLARQVEP
                     EYTPARKVHFYHCDHRGLPLALISEDGNTVWSAEYDEWGNQLNEENPYYLYQPYRLPG
                     QQYDEESGLDYNRHRYYDPLQGRYITQDPIGLAGEWSLYAYPLNPVQHVDPLGLSTMI
                     IGNGPVPDNPFGHAAAANRYGLMSSGTGDEMGASVSDYFKKMQPRRDTWIWIIDTTDE
                     EEQCMMNKAIDLNKKLKTINLGPLPVANNCFSRTNMIFEACGFSNPSMNTNAPISLQV
                     LGELYGYQRYFMPKGPFTGFPFEFQEVK"
     gene            6648..7046
                     /locus_tag="OHO85_004399"
     CDS             6648..7046
                     /locus_tag="OHO85_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673666.1"
                     /translation="MEMLGKILVIIISMIIISCDYYNAGMDYQDNIALEQVVTWDSDN
                     IFTPNIFEHNGRCFMKKNETWMLLNGDATPNIRTMSNKKIECLEKSEVEWASRVCDSI
                     TDKERENLKALICSKPAKSVNTGKRPALGY"
     gene            7110..7664
                     /locus_tag="OHO85_004400"
                     /pseudo
     CDS             7110..7664
                     /locus_tag="OHO85_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159308.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
BASE COUNT         1951 a         1977 c         2371 g         1547 t
ORIGIN      
        1 tattactcgc tgtaatgtac gagcctgtcg tgaataacgt cgagaacaca tccgttgcgt
       61 ttgtgattat tttgttgact aaacagacac ccgtttctct gaagtaaaat cccagactaa
      121 atcatcacat aaccatgaca tttttctgat attccccggt aacgccagat gtcgactcgc
      181 ttatctcatc cgcccgacat gttgtacatg caatatgtgc ggattattat tcattttcac
      241 ggaggttgct atgtcaaccg gattacgttt cacacttgaa gtggacggcc tgccaccgga
      301 cgcgtttgcg gtggtttcct ttcatctcac ccagtcactc tcttcacttt tttccctcga
      361 cctttctctg gtcagtcagc agtttctttc ccttgaattt gcgcaggtgc tggacaaaat
      421 ggcctacctg acggtatggc agggcgatga cgtacagcgc cgggtgaaag gtgtggtgac
      481 ctggtttgaa ctgggggaga acgacaaaaa ccagatgctg tacagcatga aggtgtgccc
      541 gccgctgtgg cgcacagggc tgcgccagaa cttccgtatc ttccagaatg aggacatcga
      601 aagcatcctc gctacgatcc tgaaggaaaa cggtgtgacc gagtggagtc cgctgttcag
      661 cgagccgcat ccttcccgtg agttttgtgt ccagtacggc gaaaccgatt acgatttcct
      721 gtgccggatg gcggcggagg aaggcatctt cttttatgag gagcacgcgc aaaaaagtac
      781 cgaccagagc ctggtcctgt gcgacaccgt gcgttatctg ccggagtcct ttgagatccc
      841 ctggaatccg aacacccgta ccgaggtgag caccctctgc atcagccagt tccgctacag
      901 cgcacaaatc cgcccttctt ccgtggtgac caaggactac acctttaaac gacccggctg
      961 ggcagggcgt tttgatcagg aaggccagta ccaggattac cagcgcacac agtatgaagt
     1021 gtatgactac cccggacgtt tcaagggtgc ccacgggcag aactttgccc gctggcagat
     1081 ggatggctgg cgaaacaacg cagaagtggc gcgcggaaca agccgttctc cggagatatg
     1141 gccgggacgg cgaattgcgc tgacggggca tccgcaggcg aacctgaacc gggaatggca
     1201 ggtggtggca agtgaactgc acggcgagca gccgcaggcg gtgccgggac gcagtggttc
     1261 aggtaccacg ctgaataacc actttgcggt aataccggca gaccggacat ggcggccaca
     1321 gccgttgctg aaaccgctgg tggacggccc gcagagcgcc gtcgtgacgg gaccggcagg
     1381 cgaggaaatc ttctgtgatg aacatggccg cgtgcgggtg aaatttaact gggaccgcta
     1441 taacccgtca aaccaggaca gttcatgctg gatccgtgtg gcacaggcgt gggcaggcac
     1501 cggctttggc aacctggcga taccgcgagt gggtcaggag gtgattgtgg acttcctcaa
     1561 cggcgacccg gaccagccga ttattatggg gcgtacctac caccaggaaa accgcacacc
     1621 cggcagcctg ccggggacga agacgcagat gaccattcgt tcgaaaacct ataagggcag
     1681 cgggtttaat gaactgaagt ttgacgatgc gacagggaaa gaacaggtct acatccacgc
     1741 gcagaagaac atgaacaccg aggtgctgaa taaccgcacc actgatgtga taaacaacca
     1801 tgctgaaacc attggcaaca atcagatgat tgcggttacc aacaatcaga tacagacggt
     1861 gggtgttaac cagatagaga cggtgggcag taaccagata attaaagtgg gttctgttca
     1921 ggtggaaacg attggacttg ttcgtgcgct gaccgtgggc gtggcgtatc agacgacggt
     1981 aggtggcatt atgaacacct cggtggcact gatgcagtcc tcgcagatcg gtttgcataa
     2041 atcgttgagg gtcgggctgg gttatgacgt caaagtcgga aataacgtta ccttcaccgt
     2101 tggtaaaacg aaaaaggatg ataccgggca gaccgcgatt tactccgccg gtgagcatct
     2161 ggagctctgc tgtggtaagg caaggctggt gctgacgaag gacggacaaa tttttctcaa
     2221 cggcacaaaa attcatttgc agggtaagga gcaggttaat ggtgactcac tgttgattaa
     2281 ctggaactgt gcagcctcga aatccccacc gaagacccct gatgaaaagc aggatacgcc
     2341 ggatatgaga gagtactgat cccgtacctg ttcggaattc agtaacaaac cagcattaaa
     2401 aataaacaga agaaggaatt tgaatcatga gcggaaaacc agcggcgcgt cagggagata
     2461 tgactcagta tggcggtccc attgtccagg gttcggcagg tgtaagaatt ggcgcgccca
     2521 ccggcgtggc gtgctcggtg tgtccgggcg ggatgacttc gggcaacccg gtaaatccgc
     2581 tgcttggggc gaaggtgctg cccggcgaga cggaccttgc gctgcccggc ccgctgccgt
     2641 tcattctctc ccgcacctac agcagctacc ggacgaagac gcctgcaccg gtgggcgttt
     2701 tcggccccgg ctggaaagcg ccttctgata tccgcttaca gctacgtgat gacggactga
     2761 tactcaacga caacggcggg cggagcattc actttgagcc gctgctgccg ggggaggcgg
     2821 tgtacagccg cagtgagtca atgtggctgg tgcgcggtgg taaggcagca cagccggacg
     2881 gccatacgct ggcgcggctg tggggggcgc tgccgccgga tatccggtta agcccgcatc
     2941 tttacctggc gaccaacagc gcacaggggc cgtggtggat actcggctgg tcagagcggg
     3001 tgccgggtgc tgaggacgtg ctgccagcgc cgctgccgcc gtaccgggag ctgacggggc
     3061 tggcggaccg cttcgggcgg acgctgacgt accggcgtga ggccgccggt gacctgaccg
     3121 gggaaatcac cggcgtgacg gacggtgccg ggcgggagtt ccgtctggtg ctgaccacgc
     3181 aggcgcagcg tgcggaagag gcccgcacct cttcgctatc ttcttctgac agttcccgcc
     3241 ctctctcagc ctcagcgttc cccgacacac tgcccggtac cgaatacggc cccgacaggg
     3301 gtatccgcct ttcggcggtg tggctgatgc acgacccggc atacccggag agcctgcccg
     3361 ctgcgccact ggtgcggtac acatatacgg aagccggtga actgctggcg gtatatgacc
     3421 gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcacccgggc cggatggtgg
     3481 cgcaccgtta tgcaggcagg ccggagatgc gctaccgcta cgacgatgcg gggcgggtgg
     3541 tggagcaact taacccggca ggcctgagtt accgctacca gtatgagcag gatcgcatca
     3601 ccgtcacgga cagcctgaac cggcgtgagg tgctgcatac agaaggcggg gccgggctga
     3661 agcgggtggt gaaaaaagaa ctggcggacg gcagcgtcac gcacagcggg tatgacgcgg
     3721 caggaaggct cacggcgcag acggatgcgg cgggacggcg gacagagtac ggtctgaatg
     3781 tggtgtcagg cgatatcacg gacatcacca cgccggacgg gcgggagacg aaattttact
     3841 ataacgacgg gaaccagctg acggcggtgg tgtccccgga cgggctggag agccgccggg
     3901 aatatgatga accgggcagg ctggtatcgg agacatcgcg cagcggggag acagtacgct
     3961 accgctacga tgacgcgcac agtgagttac cggcgacgac aacggatgcg acgggcagca
     4021 cccggcagat gacgtggagc cgctacgggc agttgctggc gttcactgac tgctcgggct
     4081 accagacccg ctatgaatac gaccgcttcg gccagatgac ggcggttcac cgcgaggaag
     4141 gtatcagcct ttaccgccac tatgacaacc gtggccggtt aacctcggtg aaagacgcac
     4201 agggccgtga aacgcagtat gagtacaacg ccgcaggcga cctgactgcc gttatcaccc
     4261 cggacggcaa ccggagcgag acacagtacg atgcgtgggg aaaggcggtc agcaccacgc
     4321 agggcgggct gacgcgcagt atggagtacg atgctgccgg acgtgtcatc agtctgacta
     4381 acgagaacgg cagccacagc gacttcagct acgatgcgct ggaccggttg gtacagcagg
     4441 gcggctttga cgggcggacg caacgttatc attatgacct gaccggaaaa ctcacgcaga
     4501 gtgaagatga ggggcttgtc accctctggt actacgatga atcggaccgt atcactcacc
     4561 gcacggtgaa cggcgaaccg gcagagcagt ggcagtatga tgaccacggc tggctgacag
     4621 acatcagcca cctgagcgaa ggccaccgtg tcgctgtcca ctacggctac gacgataagg
     4681 gccgcctgac cggcgaacgg cagacggtgg agaacccgga gacgggggaa ctgctgtggc
     4741 agcatgagac aacacacgcc tacaacgaac aggggctggc aaaccgcgtc acgccggaca
     4801 gcctgccgcc ggtggagtgg ctgacctacg gcagcggtta tctggcgggc atgaagcttg
     4861 gcgacacgcc gctgctggag tacacgcgcg accgcatgca ccgggagacg gtgcgcagct
     4921 ttggcagcat ggcaggcagt aatgccgcat ataaactgac cagcacatac acccccgcag
     4981 gccagttaca gagccagcac ctgaacagcc tggtgtatga ccgtgactac gggtggaatg
     5041 acaacggcga cctggtgcgc atcagcggcc cgcgacagac gcgggaatac ggctacagcg
     5101 ccacgggcag gctggagagc gtgcgtaccc tcgcaccgga cctggacatc cgcatcccgt
     5161 atgccaccga cccggcggga aaccgcctgc cggacccgga gctgcacccg gacagtaccc
     5221 tcacggcgtg gccggataac cgcatcgcag aggatgcgca ctatgtttac cactatgatg
     5281 aatacggcag actgacggag aagacggacc ttatcccggc gggtgttatc cggacggacg
     5341 acgagcggac ccaccactac cactacgaca gccagcaccg tctggtgttc tacacgcgga
     5401 tacagcatgg cgagccactg gtcgagagcc gctacctcta cgacccgctg ggacggcgaa
     5461 tggcgaaacg ggtatggcgg cgggagcgtg acctgacggg gtggatgtcg ctgtcgcgta
     5521 aaccggaggt gacgtggtac gggtgggacg gcgaccggct gacgacggta cagaccgaca
     5581 ccacgcgtat ccagacggta tatcagccgg ggagcttcgc gccgctcatc cgcatcgaaa
     5641 cagacaacgg tgagcgggag aaagcgcagc gccgcagcct ggcggagaag ctgcagcagg
     5701 aagggagcga ggacgggcac ggcgtggtat ttccggctga actggtgcgg ctgctggaca
     5761 gactggagga agaaatccgg gcagaccgcg tgagcagtga aagccgggtg tggcttgcgc
     5821 agtgcgggct gacggtggag caactggcca gacaggtgga gccggaatac acaccggcgc
     5881 gaaaagttca tttttaccac tgcgaccacc ggggcctgcc gctggcgctc atcagcgaag
     5941 acggcaatac tgtgtggagt gcagaatacg atgaatgggg caaccagctg aatgaagaga
     6001 acccgtatta cctgtatcag ccataccgtc tgccagggca gcagtacgat gaggagtcgg
     6061 ggctggatta taaccgtcac cggtactacg atccgttgca ggggcggtat atcacccagg
     6121 acccgattgg gctggcgggg gaatggagtc tgtatgcgta tccgctgaat ccagtgcaac
     6181 acgttgatcc attagggctt agtactatga taataggtaa cggccccgtt cctgataatc
     6241 cttttgggca tgctgcggcg gcaaatcgat atggattaat gagttcggga actggggatg
     6301 agatgggagc ctctgtctct gattatttta aaaagatgca accccgcaga gatacctgga
     6361 tttggataat agatacaaca gatgaagaag aacaatgtat gatgaataag gcaatagacc
     6421 ttaataaaaa actaaaaact attaacctcg ggcctcttcc tgttgcaaat aattgtttta
     6481 gccggacgaa catgattttt gaggcgtgcg ggtttagcaa cccctctatg aatacaaatg
     6541 cgcctataag cctacaagtg ctcggtgagt tatatggata tcaaagatat tttatgccta
     6601 aaggtccttt cactggattc ccatttgaat ttcaagaggt aaagtaaatg gaaatgcttg
     6661 ggaagatatt agtgataatt atttccatga taattatatc ttgcgattat tataatgctg
     6721 gcatggacta tcaagataat atagccctcg agcaagtcgt aacatgggat tctgataata
     6781 tattcacacc taatatcttt gaacataatg gaagatgttt tatgaagaaa aatgaaacat
     6841 ggatgttatt aaatggagat gcaacaccaa acattagaac aatgagcaat aagaaaattg
     6901 aatgcctgga gaagagtgaa gtagaatggg ctagtagggt ttgcgacagt atcacagaca
     6961 aggaaaggga aaatctgaag gcattaatat gtagcaaacc agctaaatca gttaatacag
     7021 gcaaaagacc agcgttagga tattgaaacc ggcactgcac tatatcacta ttacaatatt
     7081 ctgagtgact agagcaactg ctccgccatc cgggctagca gtatgacgac gaatccgaac
     7141 tgcactacaa ctggcagcgg tactacaatc cgggacaggg acgatatatc acccaggacc
     7201 cgatagggct ggcgggggga ttaaacccgt acagttatgt tatgaacaat cctgtgcaat
     7261 ggattgatcc attgggatta gctcatactt caggaaaatg gaaggactgt ggtaaaggtt
     7321 gtcgaatccg aatagatggc gatgctgttg gtgacggaag acatttgcat tgggagtgta
     7381 aaaatgggag tggtgtaatg ggggaatttg gaggtatgtc ccatggtgag gattactcat
     7441 cagcaccaaa tagcattaag aaatgtgcaa aaaaatatgg tttcgagcca gaaccaagta
     7501 aagatctgtc atgcaaaacc tccgggctat caactactga tgcggctctt gcaactggtg
     7561 ctacaattgg cgcagggtat gttatatata gagtgattcg atttatacct tcacttgcac
     7621 cacctctttg gtggtcaata cctgcaaacg ttgttactcc atgattaaaa cggataatgg
     7681 agattatata atgagcgatt ttgacattgt gagttcaatt gactggcatg atggggtttt
     7741 gttagaagta aaaacgacat tcaaagaatc tcattttaac atgacgttat ttattgacat
     7801 ttataaaagt gagaatatta gggaaaaaat gatagttgaa ttttct
//
LOCUS       DAJOWB010000178         7821 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJOWB010000178 DAJOWB010000000
VERSION     DAJOWB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.178"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            167..373
                     /locus_tag="OHO85_004401"
     CDS             167..373
                     /locus_tag="OHO85_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FaeA/PapI family transcriptional regulator"
                     /protein_id="HCQ2673667.1"
                     /translation="MKKKQILDFIQEIKTPCRTSDIASRFEMSAYQARHYLMCLEKEG
                     KIRRTPLRRGARTLWEIVRETENY"
     gene            complement(622..885)
                     /locus_tag="OHO85_004402"
                     /pseudo
     CDS             complement(622..885)
                     /locus_tag="OHO85_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174402.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1084..1584)
                     /locus_tag="OHO85_004403"
     CDS             complement(1084..1584)
                     /locus_tag="OHO85_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673668.1"
                     /translation="MQITEALLSALGEIRRFVQQGVDHWPRLLAFHFTLHSAEGNING
                     QQINAFRTSFYERITERNHTASPSSPVVLRWLREQHGGATIRCLLLLSQTSICHPRAR
                     LSVDEECSQVVDLLQQTWWMISAGGQCRVERCFRVTRPDTSEQYVALKTAVQSLMPLV
                     IATIIR"
     gene            complement(1584..1814)
                     /locus_tag="OHO85_004404"
     CDS             complement(1584..1814)
                     /locus_tag="OHO85_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673669.1"
                     /translation="MPALVLRKSALSLVLATLPFIFLLTLSGQPAGCSSFLTDSLFLS
                     SRQSLVFVSYCAGVDVRFPCVCTGLTKFTGVK"
     gene            complement(1852..2082)
                     /locus_tag="OHO85_004405"
     CDS             complement(1852..2082)
                     /locus_tag="OHO85_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCQ2673670.1"
                     /translation="MLTTISHDSVLLRADDPLIDMNYITSFTGMTDKWFYRLISEGHF
                     PKPIKLGRSSRWYKSEVEQWMQQRIEESRGAA"
     gene            complement(2151..2711)
                     /locus_tag="OHO85_004406"
     CDS             complement(2151..2711)
                     /locus_tag="OHO85_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673671.1"
                     /translation="MNAIPYFDYSLAPFWPSYQNKVIGVLERALREQSGSRIRRILLR
                     LPCEYDNTFNSRTTWFGMDFIETVSALMNATPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAKPYQKQITRDVVLDRQSPDCEALFKDLIWAF
                     SDFARDRRGVRDPEARCLAGNPGWQC"
     gene            complement(2969..3535)
                     /locus_tag="OHO85_004407"
     CDS             complement(2969..3535)
                     /locus_tag="OHO85_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCQ2673672.1"
                     /translation="MPKSYTPNWFFTALLDNHINQLVARYSRLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTADHGFHAHAVFWLDRQRVKKIY
                     PFAERITECWQSITHNSGSAHRCTYQPHYTYNINIPVRHNDPGSIDNIRGVLHYLAKE
                     EQKDGLCAYGCSEVPERPAAGRPRKPHF"
     gene            5113..5817
                     /locus_tag="OHO85_004408"
     CDS             5113..5817
                     /locus_tag="OHO85_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HCQ2673673.1"
                     /translation="MARCTAPVRGHSSAAAAAACPACRNRSGYRRSSYSYDSYPSYSS
                     GSSGGGSYGGSSGGSSSSAKPRWSKSGSSVSYTTAQVQSLTPIRQAVEKLAIEQPDLR
                     DVFLCHAWDDRQGIAKELHDLLEAAGVKVWFSEKDLALGVPMMRAIDKGLANSRIGLV
                     LVTPALLNRLPKEGVADKELSALLAGNQLVPIVHNTTYEALRNVSPLLASRSGLDTGE
                     DTLAVVATKIAELITV"
     gene            complement(6241..6972)
                     /locus_tag="OHO85_004409"
     CDS             complement(6241..6972)
                     /locus_tag="OHO85_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCQ2673674.1"
                     /translation="MNQPIHNAYWLSRFDSLLNSALAQHRAVSLIRVDLRFPEYMPAT
                     IMDTDLDSAVISRFFASLKAKIQAYQRHKRRANKRVRATTLRYFWCREFGKEKGRKHY
                     HVILLLNKDTWCSPGDFTVPSSLATLIQLAWCSALHLEPWQGNGLVHFSRRTPFRKPT
                     SSDARPSSDDTPLSGGCSETRKASDKKPGESAVLWVKRGDVEAMQKARNRASYLVKYE
                     TKQHDGSGQRNYGCSRGPGRLLDGR"
BASE COUNT         2376 a         1770 c         1700 g         1975 t
ORIGIN      
        1 cccaatcact aaccactcaa taaagcaata aatagtcaaa acagagcact actattacta
       61 acatccttta ttttcatgtg gttacaatga taaaagatgg caatgagatt caacgtaagt
      121 aaggatgtat aataaaaagg attacagcta agagtgttaa cagaagatga agaaaaagca
      181 gatactggat tttattcagg aaataaagac accatgcaga acgagtgata ttgcttcgcg
      241 ttttgaaatg agtgcttatc aggcccgcca ttaccttatg tgtcttgaaa aagaggggaa
      301 aataaggcgt acgcctcttc gtcgtggtgc ccgtacttta tgggagatag taagggaaac
      361 ggaaaattat taataaaaat atgcattttt cctgcataaa attaactcat ccctctactg
      421 aaatataacc atgatttaac aaccaagaac ttatattgtt actttacggc gttatctgaa
      481 atgttgtcta atcaagattt ttataccact ctcagataca gattactgat gcagtatccc
      541 gcagtgtcaa tactgccaac gccgcaattt ttctgatgga tggcctcttc tgcctcatta
      601 gctgtgatgt ttggataaac acctatcctg taacatctcc agcagagttg ttggtgtcag
      661 agccggcctc tctttcaaca gaggaacaag catgctgtcc cacacagctt ccagaggatc
      721 tttgcgtgtg cgccagtgcc gaacactgtt ttttgcccaa tctccttttt cgatccgacg
      781 accagaacgg actgagatac cagccttcat ggtcgagata tgctgagtta tacctttctt
      841 acgctgagtc atatagtaac tgacctgaga agtattgagc gtcacgccat ttcctgttgg
      901 atcatgtaaa tgacactcag gttacaaaac cggccagaat aatcggcgcc aggcgggctg
      961 agttattgtc gtctcatatc aattcgtgta ctcacactga agagaattct gtataaagat
     1021 tcagcagaca aaactttatc agtctgtaaa aattaacgga agagtcgata tttctcccga
     1081 tcatcaccgg ataattgttg caataaccag tggcatcaga gactgaacag ccgtttttaa
     1141 cgcaacgtat tgctctgatg tatcaggccg ggtaacccga aaacaccttt ccacccggca
     1201 ttgtccgcca gcacttatca tccaccaggt ctgttgcagt aaatccacca cttgcgaaca
     1261 ttcttcatca acgctgagtc tggcccgcgg atggcaaatg ctcgtctggc tgagcagcaa
     1321 caggcatcgt attgttgctc ctccatgttg ttcccgcagc cagcgtaata ccaccggcga
     1381 tgatggactg gcagtgtgat tacgctcagt aatacgttca taaaaggaag tacggaatgc
     1441 attaatctgt tgcccgttga tgtttccttc tgccgaatgg agcgtgaagt gaaatgccag
     1501 cagacgcggc cagtggtcca ccccctgctg aacaaaacgc cggatttccc ccagcgctga
     1561 aagtaaggct tcggttattt gcactatttt acccctgtga attttgttaa gccggtgcag
     1621 acacacggaa aacgcacatc aacaccagca cagtacgata caaacaccag tgactgacgg
     1681 gacgaaagga ataacgaatc agtcaggaaa gagctacaac ctgctggctg accggagagg
     1741 gtcagcagga aaatgaacgg taatgtcgcc agcaccaggg acagagctga tttccgcaat
     1801 accagcgccg gcattttgca cattgccagc gtaccggcat cacaactcgt ttcatgctgc
     1861 tcctcgtgat tcctcaattc gctgctgcat ccactgctct acttcacttt tgtaccagcg
     1921 gctgctgcgc cccagtttga tgggtttagg gaaatgacct tcactgatca gcctgtaaaa
     1981 ccatttatcg gtcatgccgg tgaaactggt gatgtagttc atgtcgatca ggggatcgtc
     2041 ggcacgcagc aacacgctgt cgtggcttat tgtggtcaac atggtcagaa tatcctctgt
     2101 gagattgatg aagaatgggg taatacgagg tatgggatgg aatgctgctt tcagcactgc
     2161 caaccgggat taccggcgag gcaacgggct tccggatcgc gcactccacg gcgatcgcgg
     2221 gcgaaatcac tgaacgccca gataaggtcc ttaaacaggg cttcgcagtc cggtgactgg
     2281 cgatccagta ccacatcccg ggtgatctgc ttctggtacg gctttgcttc acctggtgac
     2341 gcgaaaccca gcacgttact ccaggcatcc agaatcaacc gatccagttc ggggccgtcg
     2401 aaatactcct gtctgacgca cagcaccaca tggtattccg gtttttcagg atgacgggtc
     2461 aggagccagc aaaggtcacg tccgggtgtt gcattcatca gcgcactgac ggtttcgata
     2521 aaatccatac caaaccaggt cgttctgctg ttgaaggtat tgtcatattc acacggcaga
     2581 cgaagcagga tccgccgtat ccgtgagccg gactgctcac gcagcgcacg ctcaaggacg
     2641 ccgatgactt tgttctgata agatggccag aagggggcca ggctatagtc gaaataaggg
     2701 atagcattca taagatatgc tccgttatgc gcagcccaca atgcgtcctg cccgtgaata
     2761 agctgacgtg ttatggtttg cggatggggt tgatggaata agtgaagaac cggggcagaa
     2821 cgggaagatc aggtcaccga caagggacag aacaccggat gtggttcaga gtgaatgatc
     2881 tccgttgact tcagttcaca tcattaagca aggcggtaag gttttaccga cggaatgttt
     2941 ccaggacagg aagctcagga tttaagcttc agaagtgagg cttacgagga cgccctgcag
     3001 caggtcgttc aggaacttca ctgcaaccgt atgcgcacag tccgtctttt tgctcttctt
     3061 tcgccagata atgcaggaca ccgcgaatat tatcgatgct cccgggatcg ttatgacgca
     3121 cgggaatgtt gatgttgtat gtataatgcg gctgatatgt acagcggtgt gccgaaccgc
     3181 tgttatgcgt aatagactgc cagcattccg taatccgctc cgcaaaggga tatatttttt
     3241 taaccctctg acgatcgagc cagaaaaccg catgcgcatg aaaaccatga tcagccgtcc
     3301 attcaatcac ccagaagaaa ccaacgatat tttcaaattg ttccacctgc tccagcagca
     3361 tacgcaactg caattcaagc cagcgatgat caggttgtaa gaaatcaggc gtatctttcc
     3421 tgtagaagaa atccatgcgt aaggcccgca ggcgggaata gcgtgcgacc aactgattga
     3481 tgtgattgtc aagtaaagcg gtaaaaaacc agtttggtgt gtaagattta ggcatgctga
     3541 tgactcctga aagttaatga cgttttaagt gctgtaaacg aattaatgct ttcgctcata
     3601 atcagatcta aaacggtaat aacttacaga cggtatttat caggcatgct gtaacagaat
     3661 ggatacgtcg gttattaacg acacgtagcg ttgtgcagaa ataaatggat tatctgatgg
     3721 tattagctaa agagatgaga taaacgagga gtgaataaaa tacagaatca ataaacatca
     3781 cgaaatactg caaaatatgc gataaaaacc cacgatcaga agttcaagaa acatattacg
     3841 actaatcacc atcataaatt acaaacttgc tctcaatggt tctaaaaaac cgtgtacaag
     3901 gccaacacga tacctgtaac ccaataaact tacacgcact cattttatct gttaaaaaca
     3961 actcatgttc aagatatgaa cgttaacggg tacacggtac cagaatgaaa cataccatgg
     4021 atggataatt ttcattttgt ttaaaataca agataatcag gaacgaacaa acacaatcag
     4081 aaattcactc tattaattct gttatttacc ctgctcggct aatcgttaca gaataatgga
     4141 taccttatta aagagtaagc attcagttaa tcagtaatga cagagaaatt gatgttcacc
     4201 agaatagtta aaacacttca gataaacaat cattgttaag caaatacggt tcataatgga
     4261 taaattagaa tcaattactg ttttactgaa gatgagaata gatgaatatc aacaatagcg
     4321 tagtagagaa taacttacct gaacatcata tgagttacag tagttatgat tgagggatgg
     4381 agtgaatacg ataacaagag taatactaat ggaatgtgta aagattgaga gtgagtgaag
     4441 acaggaagta aataacaata gagattgtat gaaaaaggga gtgaataaag aatatataaa
     4501 gaataaatat aattatagta ataagcctaa attaaaatca tccttgtttg atgagtaaaa
     4561 ataattctag aaaatgaatc tgtctgaaaa ctctttctca gagattaacc gatcagatgc
     4621 tcaacggact caataaattg gctgagtcag gttacctcat ccacactcta tttgtttcta
     4681 gaaggatcta ccattttgtt catctaccgg aacacatcaa tttagtaact cgataatatc
     4741 ggttatgtag atatttatcg ttgctaccaa tgcctagatt attattctcc aagtattttc
     4801 ttgacttaca ggctaatttt gaaacaaatg tcatgctatt ttaggaaaag aacctatgca
     4861 aaagtctgct atgagcgagg agcagatatt catctggtat acaaagacaa tatcgcttaa
     4921 gtcatcagtg ctgccacttt aaccactagc tctgctcttt ttggactttt gagctaaaaa
     4981 ttaaatctga cagtgtgata aggtgcaagt ttctgaaaat acgccagttg ttttacactc
     5041 aaaagccccc attactatct gatgttaatg gcgcagcagt tatctttcag attattaagg
     5101 ggaaacaaga gtatggcaag atgtacagct cctgtacgtg gacatagctc agcagcagct
     5161 gctgctgcct gtcccgcatg tcgtaatcgc agtggttatc gccgcagtag ctacagttat
     5221 gatagttacc cttcttactc atcaggctct agcggtggcg gaagttatgg aggcagtagc
     5281 ggtggttcca gcagcagcgc gaagccacgt tggtcaaaat cagggtcttc cgtttcttac
     5341 acaacagccc aagtacaatc actcacacca attcgacaag cggtagagaa acttgccatt
     5401 gagcaacctg atctgcgtga tgtctttctt tgccatgctt gggatgaccg acaaggtatt
     5461 gccaaagagt tacacgattt gctcgaagcg gcaggtgtca aagtatggtt cagcgaaaag
     5521 gatcttgccc ttggtgttcc gatgatgcgt gccattgata aaggattagc aaactctcga
     5581 atcggcttgg ttttagtgac acctgcattg ttaaaccgtc ttcctaaaga aggcgttgct
     5641 gataaagaac tttccgcctt actggcaggc aaccaactcg ttcccatcgt gcataacacg
     5701 acgtatgaag ctctccgtaa tgtcagtcct ttgcttgcat cccgaagcgg cctcgacacg
     5761 ggagaagata cattggcagt tgttgcaaca aaaattgcag agctgatcac tgtttagttt
     5821 atccaagcca ccgttaaata tttccagctc atgaatctgg tctgctattg caggccagat
     5881 atcagtcaat actgaaaggt ttgacctgct ccccgttgat taacatagac tgatatagca
     5941 acttccgctt ttggcacaaa gcagaccatg agaagataga cgctataaca tttgagtcct
     6001 accaatcgat ctgttcattt ccataaatgg cagtaaatgc aaataaccag aacagccagg
     6061 cgcttttcga cttatggtcg atgttgcaaa gagtgaggag tggaaagcaa gcgtgcgaac
     6121 gaaagtagat ggcaatccat tgcattactt caggacgact tttcagaaag cgagctaagg
     6181 gcgtcagcca acgcccttac cgtggcataa gctcaggcac cggatgccgt tttacagggc
     6241 tcacctgcca tccagtaaac gccccggccc acgactgcaa ccataattac gttgcccgga
     6301 accgtcatgc tgcttcgtct catatttcac gagataactg gctctgttcc gggctttctg
     6361 catcgcttcc acatcaccac gcttgaccca gagcacagcg gattcacccg gctttttgtc
     6421 tgaagccttc ctagtttcag aacatccacc cgacaaaggc gtatcatcgg aagaagggcg
     6481 agcatcagat gatgtcggtt tacggaaagg cgtccgcctg gaaaaatgaa ccagtccatt
     6541 accctgccag ggctcaagat gcagagcact acaccatgcc agttggatca gcgtcgccag
     6601 cgaagaagga acggtgaaat cccctggcga gcaccaggta tctttgttga gcagtaatat
     6661 cacgtgataa tgtttcctgc ctttttcctt gccaaactcc cgacaccaga aataacgcag
     6721 agtggttgca cgcacacgct tgttggcacg tcgtttatgt cgctggtaag cctgaatttt
     6781 tgctttcagg gatgcaaaaa aacgagaaat caccgctgaa tctaaatcgg tatccatgat
     6841 ggtggctggc atatactcag ggaaacgtaa atccacccgg attaacgaga cggcacggtg
     6901 ctgcgccagg gcactgttga gaaggctgtc aaaacgggat aaccagtaag cattgtgtat
     6961 tggttgattc atgatgagag atctccgttg atttggattg acttcacatc attaatcact
     7021 gcggtaaaac tttacggcat cccgccattg agcaacgcca atggctcctg attaaaacag
     7081 aatgaatccc ccacgaagaa aaaataaacc tacacgcaga gtaaaacaca caacaacggc
     7141 ataaatgttt tttaaaaact acaaagcctg aagggaaacg gatcaacaga acgcgcaaac
     7201 aaaacgtcag aaacgcaatc agaacgcttt ctggcgctgt aaatcaacag caacaccaca
     7261 cctacagctt aaccacacaa ctcaatcaca gagccgaaca gcgcgattaa aacctgttta
     7321 cagacacccc cgctccaaga acaaaaacca ccaacatcag aatcgatttt cttatactga
     7381 aaacgttact tttaactacg ctccataaaa acggttaacc atcgataacg taaacctcat
     7441 ctgaatcaaa acaccagaac caatcatcac gcttttcctg aaaagagtct tgcgctgaga
     7501 aaaaacacca gggccagtta atcatctcat caatctcatc gttacaaaat catccataaa
     7561 accatttaac aaaatcatcg taagcacaca gtaaatcacg ccaggttatc tcagccggat
     7621 ttgcgttttt acataaagac acttgtctca ggttaacgac taaaaacaca aaaactaaga
     7681 aaaggttaaa cgtaacttca gaaaaataga atatcatcct gattacttaa acaggaaaga
     7741 ttgttttgta gcagctttaa actgatttta gctaacgtaa agatttacta aactggatgt
     7801 gtttcagaca aagcaatgtg t
//
LOCUS       DAJOWB010000179         7644 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJOWB010000179 DAJOWB010000000
VERSION     DAJOWB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.179"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            9..1223
                     /locus_tag="OHO85_004410"
     CDS             9..1223
                     /locus_tag="OHO85_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2673675.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYLCTFPPHPGHRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            1450..2715
                     /locus_tag="OHO85_004411"
     CDS             1450..2715
                     /locus_tag="OHO85_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2673676.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            2727..3593
                     /gene="ydiB"
                     /locus_tag="OHO85_004412"
     CDS             2727..3593
                     /gene="ydiB"
                     /locus_tag="OHO85_004412"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCQ2673677.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            3624..4382
                     /gene="aroD"
                     /locus_tag="OHO85_004413"
     CDS             3624..4382
                     /gene="aroD"
                     /locus_tag="OHO85_004413"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCQ2673678.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            4527..6122
                     /locus_tag="OHO85_004414"
     CDS             4527..6122
                     /locus_tag="OHO85_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCQ2673679.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            6136..7287
                     /locus_tag="OHO85_004415"
     CDS             6136..7287
                     /locus_tag="OHO85_004415"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCQ2673680.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(7330..>7644)
                     /locus_tag="OHO85_004416"
     CDS             complement(7330..>7644)
                     /locus_tag="OHO85_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2673681.1"
                     /translation="EQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTN
                     TELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSL
                     VG"
BASE COUNT         1969 a         1739 c         1848 g         2088 t
ORIGIN      
        1 tagaacctat gaaaaatccc tattacccta ccgcacttgg gttgtatttt aattacctgg
       61 tgcatggtat gggcgtcatt ttgatgagcc tgaatatggc ctcgctggag acactttggc
      121 agactaatgc cgcgggtgtc tcgatagtta tctcatcgct gggcattggt cgattaagtg
      181 tcttgctttt tgcaggatta ttatccgatc gctttggtcg ccgccctttt atcatgctcg
      241 ggatgtgctg ctatatggcc ttcttttttg gcatcctgca caccaataac atcattatcg
      301 cttatgtttt tggctttctg gcaggaatgg caaacagttt tctcgatgca ggcacttatc
      361 ccagtttaat ggaagctttt ccacgctcac ctgggacagc caatatttta attaaagcat
      421 ttgtttccag cggacaattt ttattaccgc taatcattag cctgttagtg tgggctgaac
      481 tgtggttcgg ttggtccttt atgattgctg caggcattat gtttattaac gctctgtttt
      541 tatacctttg tacgttccca ccccatccgg gtcatcgctt acctgtcata aagaaaacca
      601 ccagctctac ggaacatcgc tgttcaatta tcgatttagc cagttatacc ttatatggct
      661 atatctcaat ggcaacgttt tatctggtta gccagtggct ggcacagtac ggacaatttg
      721 ttgcaggcat gtcatacact atgtcgatta aactactcag tatctacacc gtgggttcgc
      781 tgctttgtgt atttattacc gctccactca ttcgtaatac cgttcgccca acaacattac
      841 tgatgctgta cacctttatc tcatttattg ctctgtttac cgtctgcctg catcccacat
      901 tttatgtggt gataatattt gcttttgtca ttggttttac ctctgctgga ggtgttgtgc
      961 aaattggcct gacgttaatg gctgaacgtt tcccttacgc taaaggtaaa gctacaggga
     1021 tctattacag tgcgggcagt attgcgacct ttactattcc gttgattacg gctcatctgt
     1081 cccaaagaag tattgccgat attatgtggt tcgataccgc catcgctgcc atcggttttt
     1141 tactggcact gtttatcggc ttacgcagcc gcaaaaaaac gcggcatcac tcgctaaagg
     1201 aaaatgtcgc tccgggtggg taatgcaata ttcttttcag gtcatgcaag atcttacgga
     1261 taaataactc tttctgcgct aactaaggaa aatcgcgatc aaaaacaaac tatgacatgc
     1321 aatattcctg gaaacataaa ctttatgcca tgtacccagg aaaaatcatc ttcagtatag
     1381 taattatgta aaccgtcgaa gaacaatacg tacggtaacg aaattatctt tcagcaagga
     1441 gctgtgaaaa tgtctcaaaa taaggctttc agcacgccat ttatcctggc tgttctttgt
     1501 atttacttca gctacttcct gcacggcatt agtgttatta cgcttgccca aaacatgtca
     1561 tctctggcgg aaaagttttc cactgacaac gcgggcattg cctacttgat ttccggtatt
     1621 ggtttggggc gattgatcag tattttattc ttcggtgtga tctccgataa gtttggtcgt
     1681 cgggcggtga tattaatggc agtaataatg tatctgctat tcttctttgg tattcccgct
     1741 tgcccgaatt taactctcgc ctacggtctg gcagtgtgcg taggtatcgc taactcagcg
     1801 ctggatacgg gtggctaccc cgcgctcatg gaatgctttc cgaaagcctc tggttcggcg
     1861 gtcatactgg ttaaagcgat ggtgtcattc ggacaaatgt tctacccaat gctggtgagc
     1921 tatatgttgc tcaataacat ctggtacggc tatgggctga ttattccagg catcctattt
     1981 gtactgatca cgctgatgct gttgaaaagc aaattcccca gccagttggt ggacgccagc
     2041 gtagctaatg aattaccgca aatgaacagc aaaccgttag tctggctgga aggtgtttca
     2101 tcggtactgt tcggtgtagc cgcattctcg accttttatg tgattgtggt gtggatgccc
     2161 aaatatgcga tggcttttgc tggtatgtca gaagctgagg cattaaaaac catctcttat
     2221 tacagtatgg gctcgttggt ctgtgtcttt atttttgccg cactactgaa aaaaatggtc
     2281 cggcccatct gggctaatgt atttaactct gcactggcaa caataacagc agccattatc
     2341 tacctgtacc cttctccact ggtgtgtaat gccggagcct ttgttatcgg tttctcagca
     2401 gctggtggca ttttacagct cggcgtttcg gtcatgtcag agtttttccc caaaagcaaa
     2461 gccaaagtca ccagtattta tatgatgatg ggtggattgg ctaattttgt tattccgctg
     2521 attaccggtt atctgtcgaa catcggcctg caatatatca ttgttctcga ttttactttc
     2581 gcgctgctgg ccctgattac cgcaattatt gtctttatcc gctattaccg cgttttcatt
     2641 attcctgaga atgatgtgcg gtttggcgag cgtaaattta gcacccggct aaacacaatt
     2701 aagcatagag gttaaaggag ttaattatgg atgttaccgc aaaatacgaa ttgattgggt
     2761 tgatggctta tcctatccgc cacagtttat cgcctgaaat gcagaataaa gccttagaaa
     2821 aagcgggatt gccatttacc tatatggcct tcgaagtgga taacgatagc tttcccggag
     2881 caattgaagg attaaaagct ctaaaaatgc gcggaactgg tgtatcaatg ccgaacaaac
     2941 aactggcgtg tgaatatgtt gatgaattaa cgccagcagc caaactggtg ggtgccatca
     3001 acaccatcgt taatgatgat ggctacctgc gcggctataa caccgacggc acgggccata
     3061 ttcgcgccat taaagagagc ggttttgata tcaaaggcaa aacaatggtg ctgttagggg
     3121 ccggaggtgc ctcaacggcg attggcgcgc agggggcaat tgaaggttta aaagaaatta
     3181 aactctttaa ccgtcgggat gagttcttcg ataaagccct cgccttcgcg cagcgggtta
     3241 acgaaaacac cgattgtgtc gtcacggtta ccgatctcgc cgatcaacaa gcctttgctg
     3301 aagccctggc ttccgccgac attttaacca atggcacaaa agtgggtatg aaaccccttg
     3361 agaatgaatc attagttaat gatatcagtc tgttacatcc gggactgctg gtcactgaat
     3421 gcgtgtataa cccgcatatg acgaagttat tgcagcaggc gcaacaagct ggttgcaaaa
     3481 cgattgatgg atacggcatg ttgttgtggc aaggggctga acagttcacg ttatggactg
     3541 gcaaagattt ccctctggaa tatgttaaac aggtcatggg gttcggtgcc tgacaggctg
     3601 accgcgtgca gaaagggtaa aaaatgaaaa ccgtaactgt aaaagatctc gtcattggta
     3661 cgggcgcacc taaaatcatc gtctcgctga tggcgaaaga tatcgcccgc gtgaaatccg
     3721 aagctctcgc ctatcgtgaa gcggactttg atattctgga atggcgtgtg gaccactttg
     3781 ccgacctctc caatgtggag tctgtcatgg cggcggcaaa aattctccgt gaaaccatgc
     3841 cagaaaaacc gctgctgttt accttccgca gtgccaaaga aggcggcgag caggcgattt
     3901 ccaccgaggc ttatattgct ctcaatcgtg cagccatcga cagcggcctg gttgatatga
     3961 tcgatctgga gttatttacc ggcgatgatc aggttaaaga aaccgtcgcc tacgcccacg
     4021 cgcatgatgt gaaagttgtc atgtccaacc atgacttcca taaaacgccg gaagccgaag
     4081 aaatcattgc ccgtctgcgc aaaatgcagt ccttcgacgc cgatattcct aagattgcgc
     4141 tgatgccgca aagtaccagc gatgtgctga cgttgcttgc cgcgaccctg gagatgcagg
     4201 agcagtatgc cgatcgtcca atcatcacga tgtcaatggc aaaaactggc gtaatttctc
     4261 gtctggctgg tgaagtattt ggctcggcgg caacttttgg tgcggtaaaa aaagcctctg
     4321 cgccagggca aatctcggta aatgatttgc gcacagtatt aactatttta catcaggcat
     4381 aagcaataat atttcggcgg gaatatcctc ccccgccgaa ctaaaaaaat atattcagcc
     4441 gtatttaata aaaatatttc gtgagtctct gtgcgctaat tatccatctg gcgtagggaa
     4501 aatcacacct gaatcaggaa ttaacaatga aacctgtaaa accacctcgt attaatggac
     4561 gagtgccggt cctgtcggca caggaagcgg tgaattatat tcccgacgaa gcaacacttt
     4621 gtgtgttagg cgctggcggc ggtattctgg aagccaccac gttaattact gctcttgctg
     4681 ataaatataa acagactcaa acgccacgta atttatcgat tattagtccg acagggcttg
     4741 gcgatcgcgc cgaccgtggt attagtcctc tggcgcaaga aggtctggtg aaatgggcat
     4801 tatgtggtca ctggggacaa tcgccgcgta tttctgaact cgcagaacaa aataaaatta
     4861 ttgcttataa ctatccacaa ggtgtactta cacaaacctt acgcgccgcc gcagcccacc
     4921 agcctggtat tattagtgat attggcatcg ggacatttgt cgatccacgc cagcaaggcg
     4981 gcaaactgaa tgaagtcacg aaagaagacc tgattaaact ggtcgagttt gataacaaag
     5041 aatatctcta ttacaaagcg attgcgccag atatcgcctt cattcgcgct accacctgcg
     5101 acagtgaagg ctacgccact tttgaagatg aggtgatgta tctcgacgca ttggttattg
     5161 cccaggcggt gcacaataac ggcggtattg tgatgatgca ggtgcagaaa atggttaaga
     5221 aagccacgct gcatcctaaa tctgtccgta ttccgggtta tctggtggat attgtggtgg
     5281 tcgatccgga tcaaagccaa ctgtatggcg gtgcgccggt taaccgtttt atttctggtg
     5341 acttcaccct tgatgacagc accaaactta gcctgcccct aaaccaacgt aaattagttg
     5401 cgcggcgtgc attattcgaa atgcgtaaag gcgcggtggg gaatgtcggc gtcggtattg
     5461 ctgacggtat tggcctggtc gcccgggaag aaggttgtgc tgatgacttt attctgacgg
     5521 tagaaacagg tccgattggc ggaattactt cacagggtat cgcctttggc gcgaacgtga
     5581 atacccgcgc cattctggat atgacgtccc agtttgattt ttatcacggt ggtggtctgg
     5641 atgtttgtta tttgagtttt gctgaagtcg accagcacgg taacgtcggc gtgcataaat
     5701 tcaatggcaa aatcatgggt accggtggat ttattgatat cagtgccacc tcgaagaaaa
     5761 ttattttctg cggcacgtta actgcgggca gtttaaaaac agaaattgcc gatggcaaat
     5821 tgcatatcgt ccaggaagga cgggtaaata aatttattcg ggaactaccg gaaattactt
     5881 ttagcggaaa aatcgcgctc gaacggggac tggatgttcg ttacatcacc gaacgtgctg
     5941 tattcacgct gaaagaagac ggcctgcatt taatcgaaat cgcccctggc gtcgatttac
     6001 aaaaagatat tctcgacaaa atggacttca ccccagtgat ttcgccagaa ctcaaactga
     6061 tggacgaaag attatttatc gatgcggcga tgggttttgt cctgcctgaa gcggctcatt
     6121 aataggagta taatgatgga tttttcttta actgaagaac aagaactgct gctggccagt
     6181 attcgcgaac tgattacgac taactttccg gaagagtatt tccgcacctg cgatcaaaac
     6241 gggacatatc cgcgtgagtt tatgcgggcg ctggcggata acggtatttc catgcttggc
     6301 gtgccggaag aatttggtgg tatccctgcg gattacgtca cccaaatgct ggcgctgatg
     6361 gaagtgtcaa aatgcggtgc tccggcattt ttgatcacca acggtcaatg tattcacagt
     6421 atgcgccgtt tcggttctgc agagcagcta cgtaaaacgg cagaaagcac cctggaaacg
     6481 ggtgatcccg cctatgccct ggcgttgacg gaaccaggtg ctggctcaga taacaacagt
     6541 gccactacca cttacacgcg taaaaacggc aaggtctaca tcaacggaca aaaaaccttt
     6601 attaccggtg cgaaagagta cccgtatatg ctggtgttgg cgcgcgatcc gcaaccgaaa
     6661 gatcccaaaa aagccttcac cctgtggtgg gtcgactcca gtaagcccgg cattaagatt
     6721 aacccgctgc ataaaatcgg ctggcatatg ctcagcacct gcgaagtcta tctcgacaac
     6781 gtggaggttg aagagagcga catggtgggc gaagaaggaa tgggtttcct caatgtgatg
     6841 tacaactttg agatggagcg cctgatcaac gccgcgcgca gcaccggctt tgccgaatgc
     6901 gcctttgaag atgccgcccg ctatgccaac caacgtatcg cttttggtaa gcccattggt
     6961 cataaccaga tgatccagga aaaactggcg ctgatggcga ttaagatcga caacatgcgc
     7021 aacatggtgc tgaaagtggc atggcaagcc gatcagcatc agtcactgcg caccagcgcg
     7081 gcgctggcaa aactgtattg cgcacgtacc gcaatggaag tcattgatga tgcgattcaa
     7141 atcatgggcg gtctgggcta taccgatgaa gcgcgcgtct cccgcttctg gcgtgatgtc
     7201 cgttgtgaac gtatcggcgg cggtacagac gaaattatga tttacgtagc aggtcggcag
     7261 atcctgaaag actatcagaa caaataatct gcaggcggcg cagcttctta acaaactgcg
     7321 ccgccagatt tatccaacaa gacttaccgg ttgaggaaat tcagatagga tttcttgttc
     7381 cgcaaaacag tttttaaatt gtctacggta atcgctgggc gaacaaccga catggcgcaa
     7441 aaacagtttg gcaaagtgat cgacattttc ataacccaca cgccaggaga tctctgcctg
     7501 tgataattca gtattagtga gtgaccattt tgcttccgtc atacgccgtt gaataacata
     7561 gttaataggg gaaatacgat actctttggt aaattcatgg caaatataac tgacgctggc
     7621 acgaaatttt ttcgataact gttc
//
LOCUS       DAJOWB010000180         7508 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJOWB010000180 DAJOWB010000000
VERSION     DAJOWB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.180"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(355..4767)
                     /locus_tag="OHO85_004417"
                     /pseudo
     CDS             complement(355..4767)
                     /locus_tag="OHO85_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
     gene            complement(4787..5248)
                     /locus_tag="OHO85_004418"
     CDS             complement(4787..5248)
                     /locus_tag="OHO85_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HCQ2673682.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5276..7177)
                     /locus_tag="OHO85_004419"
     CDS             complement(5276..7177)
                     /locus_tag="OHO85_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCQ2673683.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1444 a         2264 c         1997 g         1803 t
ORIGIN      
        1 taatatttca tttagaacgt gttcctccct agataaacgt cctagtgctt cgtatgcttc
       61 tcgtccaatt ttcgcaaatt gcttagcatg aatcacttca tgatacacct catataaggt
      121 agcatttttc ctaagataaa tattgcgaag gccataatca aaagctgccc tagcatttgg
      181 tggcaaatat ttatctgctt tccttattac tttaattcct agcttctcca tttctttagc
      241 aatattgttt atttgactac gtgataaacg aaccccatcc aggaaacccg tatgcccact
      301 aacacgacga ccatcgccaa tatagtccat caaactgctg attttagaac ttgcagccaa
      361 tcccagcgga tcaacccacc cacacggatt tggcacatac cggtaagtct gctcaccccc
      421 cagcaacccc agcggatcac tcaccaggta catcccggtt tccggctcgt aataccggaa
      481 ccggttatag cacagcccgg attccgcatc ctgccactgt cctgcataca gcaggccggg
      541 ggcggcttcc gggtccagtt ccccgcgcgg gtccgggtag ccggtgtctg cgggcaggct
      601 gagtgccagc ccccacaggc tggtctgccc cggtcgccag acggttttac cttcactgtt
      661 aaagagcatc agcggacggc ccgtcaggtc gctcaccgca tgattcgtcc gcgtcaccca
      721 ctgcggggcc accgacggat gcggctgccg tacccggctg aactgctggc ttatcagctc
      781 aaagccgtta aacaccaggt gccgtacgct gtacagttta tcatcgcggt attcccggat
      841 ttccgcaata ctgtcgccgt cccacagata cgtgaagcgg attttcttcc ggtcgcagcg
      901 tttctctgtt cgtctgccgc tggcgtcgtg gcggtattcc cacagctcac cctgtgcgct
      961 gcaatacccg gtcagctggt cacggctgtc ccaccggaac cgctctgttt ccggtcggta
     1021 gccgtcacgg tgtctggtgc ggctgaccat ccggcctgcg gcgtcatagt catactgcgt
     1081 gtttccggcc tgtttcagcc ggtggcctcc ggcatactgg tcagtctctt cacttatccg
     1141 gtgcctgccc gcagactgcg ccttcaggta gccgcagctg tcatagccat aggactcatc
     1201 gccttcgcca tagcccagcc cggtgcctga ggctgtcacc gccatgacct ggccgttccg
     1261 gtccagccgg tactcccgcc gtgtttcccg tccgtaatct tcccggctgg tgacagccga
     1321 cacattaccc gcgctgtcgt aacggtactc ccgtaccagt gagggaacca gcccgcccca
     1381 tgtggcttct tccggaaaaa atccggcgcg ctggctgaca ggctgaccgg ctgcattcca
     1441 gccctgttca caggcaaaac cccggttact gtgccggagc acttcacgtc ccgcctcatc
     1501 acggctgaac gtcagcgtct catcccccgg caggataacc tctgccagtt ctccggcggc
     1561 gttgtaacgg taggtggttt tcagcaggga ttcatcggga tgtctgtcgc gttcatcttc
     1621 cggtggcagg atacagcgga tgacggtgtg ttcatcgggg tagtggtatt caatggcccg
     1681 tcggccctga gtggtgacat tcacccgccc gcgttcgtca tagccggtgc gctgtgatat
     1741 gccgtccggg ctgaagatac ccgtcagcct gccggatttg tcatacgcat actgcgtttt
     1801 gccttccggc gtgatgattt ctgccaggcg gcctgcggca tcccggctgt agaggatggt
     1861 ttcctccaga ccgttaacct ttgcggtata caggccatcg gcgtcatagc ggtaacgcca
     1921 gaccacgccg ccccagtcat gctcttccgt cacccggccg cagttatcca gaacataaag
     1981 atatgactca ccctgcgggt tgatgacttc tgtcagttgc agcgtgtctt tatcgtagcg
     2041 gtactgccag cggtggcctt caccgtccgt tcgcgccacc ggcaaatcga acggaccgta
     2101 ttccatcgtc cagacaacgc cgttaccgtc cgtccaggcc agcaggttgc cgtggcggtc
     2161 atagcggaat ccttccgcgc cggttccagc acgttcaatg ccactcagtt tatggtgctg
     2221 ctgccactgt aaccgcactg actgaccgct ggggccgcgc atcttcaccg gcaggtcgtg
     2281 accgctgtat tcggtgtggt gttcacggcc catcacgtcg gtgagtgttt ccggccggtt
     2341 gtggtggtcg tactctatgc ccagacgccg ttcatcctgg cggaatatcc ccgacagcag
     2401 cccctgcggc gtgtacgcca gccgggtctc ttcgccctgt ggcgacacca gacttaccgg
     2461 caggccgcgt tcatcacact gctgcgtcca cgtctgtccg gcagggtcgg tgatgttgct
     2521 gagcaggccg cgctcatcat agcagtactg ccactccgcg ccgtctgccg ccgtgtaacg
     2581 gcttaactga ccgtgtgcgg cgttgtattc aaacttctcc acccgcccgg caggggagtg
     2641 acgggccagc aggtgatgga attcatccca ttaatacgtg gtggtgctgc cgtccggcgc
     2701 ttcttcccgc agaatattgc cgtcaggatc atagtaataa cggtgagtgc ccttgccgtc
     2761 cgtcacggtg gtgcagccgt caccatagtc cagcgtggcg ttgtaatagc cctccgcccc
     2821 ggtgacggtc acgcaccggc cctgttcatc gtaggtgaaa cggctccacg tctggtcgtt
     2881 atcggaccag cggatgatcc ggtccgcagc gtcgtactca taaaacaggt ggtagtccag
     2941 ccgcgcatcc gcttctgtca gccgcccccg cgcatcctgt tcgtaggtcg ccagcgtctg
     3001 tatgccgtta tccgtgcggt gaatggcttt caggtagccg ctggcgtggt acatcagttt
     3061 aagccggatc ccgtcggtgt gactgacacc cgtgatgcgg tactggctgt cgcgctcaaa
     3121 cgtgatgcgg ttgctggcgg tgtcgctcat cccgcccagc atatacatcc ggtcgtcggg
     3181 gctgtgtgcc aggcggtcat cgctggtatc gtcagtcacc gggtggcgca gacgcattcc
     3241 cggaaaggcg tcataaaaga agcggctgct cagtgcatcc ctgtcgaaca gctccaggta
     3301 atcgtcgcgg cggcgcagaa tgtactgggc gtgatacggg ttaaccgccg tatcactgtc
     3361 accgtcgaag gcgaagttca gcgtggctcc ctggctgata atatctaccc ggtttccctg
     3421 ttcacgcacc caggcgtatt cgctccagga gtcgctccag cccacgccgg tcaggccatg
     3481 cagcggcagt acagaactgt ggaaacgggt gaaggagagg gggagtgtct ggcccagggt
     3541 gaagtcggta cgttcgtcgc agtacgcgcc ggttaccgga tcgacaggat ctgttaccca
     3601 tctgatgact ttattggcaa tactttttaa cgacttacct gttttcccca gcaacgcgcc
     3661 gagctttgcc cccagcagct tcccgctttt ccccagcagg ttccccagtt tgccgagtct
     3721 cgccagattc ttcagttttc ccagcagtcc cgctggcggc agaagatagc tggctatcgt
     3781 tattcccccc aggatcattc tctgccacgg cgggaactcc ggctggatat ccagataggc
     3841 cacctgctca ccgccaataa agacgtccgg cgaacctccc acgattgctg tgctgcactc
     3901 cagtttgtcg ccctggcgtg cggcaggctg attgttgata aagacgttgc tgctgccctg
     3961 ggctatcctc ggtggtttgt tctccttgtc gcagagcgcc gtgtcctcac cggcttccgg
     4021 gaaatccaca tcttttttga tttcgccacg cccgcccacc atggtggcaa actccattaa
     4081 taccggaccg gcgggaaaca gaagcgatgc gccggcgctg acgcgatcca taaaaccggc
     4141 attgctgtcc gtcagggtga taacagcgtt cccccaggcc ttcgccagtg gtactgcttt
     4201 ttccgctgcc gattcaagca ggtcagtggc aatatcaccc catgagggtt ccccctgtgg
     4261 gacttcaggt attgctccag gtgggggagg agggggaagg gtgacggcgg ccctggcagc
     4321 gggctttccg ttaattttta cgttttcaga accggtattg atggcgccgt ccgtgtcaca
     4381 aaaactgttt atccatgact caagctggtt ctgtttctcc tccacataat cactcgcctt
     4441 atagctggca tacatcagcg caggcaccag aaccagcgca taagggccgg tggcacaggc
     4501 ggccataatc aggcaacctt ctgcgatggt cactatcgcc cccagcacgg cccctgccag
     4561 cgcccccaaa aaactcttgt gctgaatttc atcgcccagc ctggcggcgg gggggccaga
     4621 cttttttgat gccagcatct gtgcaatggc tttgtcgttg gctgcccgtt tcatctggct
     4681 ggcgaccatc gcctgctgtg acagcattcg cattgccagc gtaccgcctg cggtcgctcc
     4741 ctgtggcccg cctggtcctt cactcatgtg ttatccctcg tttatgtcag cgcggctggt
     4801 ccttttggat gtcattctcc ggtggcaacg gggtgaagct ctttatcacc tccagcagca
     4861 tcgtcttttg actgttcttc agggataagg aggtgctgaa agtgaaggtc agcagcgtat
     4921 ctccacgccg ctggaatacg gctatctggc taatccgccc ctgattcccc tcatcttccg
     4981 gaccgttcca ggaataatcc agcagccagg ccggggcctc tgccagagtg atttcccggc
     5041 aggcatgttc ggtgtaccca tcaagtttgc cctggtacaa cgccagcgtt tcctggtaca
     5101 gctctggtgt cagggttccc tgtggaacgg cttcgcgggt aatgaccagg ttgatgccac
     5161 tgttatctgt ggacaccagg acctgcattg actgatcctg ccattgagac ggcagttgaa
     5221 tgtggccttc attaaaagtg aactgcataa aatactccct gataatctga gtgaatcagt
     5281 tgatgaaaac agaaccgccc acgatggtga tgccatcctt cgacagcctg attaccgtcg
     5341 attccccgac ctgtagctga atgccatccg gcgtcagcgc aattttgctt tcctccacag
     5401 ccagcaaaat ttccttaatc gccgtataag cctgtatacc gtcgccttcg ccaccattgt
     5461 ttacagcgtc aacatgcacc gaaccggcaa tcgtgcctct gtcctgaaag acatcctcag
     5521 ataaagaccc tttcagtact ccggtgatct tgtggccttt ttccaccgta agaaggcggt
     5581 taccttcctg gatcgtgacc gtctggtcat tttttacggt ttcgcggtga tcgtgtttta
     5641 cggtggtggt acggtcgttg agcacttcgg tatccatgtt cttctgcgcg tggatgtaga
     5701 cctgttctct ccctgtcgca tcatcaaact tcagctcatt aaatccgctg cccatgtacg
     5761 ttttggagcg gatggtcatc tgcgtctttg ttcccggcag gctgccgggg gtgcggtttt
     5821 cctggtggta ggtgcgcccc ataatgatcg gctggtccgg atcgccgttg aggaagtcta
     5881 caatcacctc ctgacccaca cgcggtatcg ccaggtggcc aaaaccggtg cctgcccacg
     5941 cctgtgccac acggatccag cacgaactgt cttggtctgc cgggttataa cggtcccagt
     6001 tgaacttcac ccgcacgcga ccatgttcgt cgcagaagat ttcctcgcct gccggtcctg
     6061 tcacgacagc gctctgcggg ccgtcgacca gcggtttcag caacggctgt ggtcgccatg
     6121 ttctgtctgc cgggatcacc gcaaaatggt tctccagcgc cgtccccgct ccctgccgtc
     6181 ctggcaccgc ctgtggctgt tcgccgtgca gttcacttgc caccacctgc cattcccggt
     6241 tcaggttcgc ctgcggatgc cccgtcagca caattcgccg tcccggccat atctccggcg
     6301 agcggctcat tccccgcgcg gtttctgcgt tgtttcgcca gccgtccatc tgccagcggg
     6361 caaagttctg cccgtgggcg ctcttgaaac gtccggggta gtcatacact tcatactgcg
     6421 tgcgctggta gtcctggtgc tggccttcct gttcaaaacg tccggcccag ccggggcgtt
     6481 taaaggtgta gtctttggtc accacggaag aagggcggat ttgtgcgctg tagcggaact
     6541 ggctgatgca gagggtgctc acctcggtac gggtgttcgg gttccatggg atttcaaaag
     6601 attcgggcag atggcggact gtgtcgcaca gcaccaggct ctggtcggta cttttgtaag
     6661 catgctcctc ataaaagaag atgccttcct ccgccgccat ccggcacaga aaatcgtaat
     6721 cagtctcacc gtactggaca caaaactcac gggaaggatg cggctcgctg aacagcggac
     6781 tccattcggt caccccgttt tcctgcaaca tcgtgccgag gatgcttttg atgtcctcgt
     6841 tctggaagat acggaagttc tggcgcagac cggcacgcca cagcggcggg tgcaccttca
     6901 tgctgtacag catctggttt ttgtcgttct ccccgagttc aaaccaggtc accacgcctt
     6961 tcacccggcg ctgtacttca tcgccctgcc atatcgtcag gtaggccatt ttgtccagca
     7021 cctgcgcaaa ttcaagggag agaaactgct ggctgaccag ggagagatcg agggaaaaga
     7081 gcgaagagag tgactggttc agatgaaagg aaaccaccgc aaaagcatcc ggcggcaggc
     7141 cgtccacttc cagtgtgaaa cgtaatccgg ttgacatagc aacctccagt aaaatgaata
     7201 atgatccgta ccgtacaact gcggataatg tgataagcgg gacgacttca gacgttatta
     7261 gggaaggaac agaaaatgct atggttatgt gagaattttg tttaaatatt actttacaga
     7321 aacgggtgtc tgttcagtca aaaaaataat cacaagctca acctcttgtt tcttggcgtt
     7381 attcaggaca ggtacgcata tcacaacgag taacaaaaac gtaaagttaa accaatattc
     7441 ataatatatt tttattcaca gcaataaata atccaattaa cattattaaa atgaatgatt
     7501 gcatgaga
//
LOCUS       DAJOWB010000181         7460 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJOWB010000181 DAJOWB010000000
VERSION     DAJOWB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.181"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            13..144
                     /locus_tag="OHO85_004420"
     ncRNA           13..144
                     /ncRNA_class="other"
                     /locus_tag="OHO85_004420"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            191..322
                     /locus_tag="OHO85_004421"
     ncRNA           191..322
                     /ncRNA_class="other"
                     /locus_tag="OHO85_004421"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(506..1183)
                     /gene="narI"
                     /locus_tag="OHO85_004422"
     CDS             complement(506..1183)
                     /gene="narI"
                     /locus_tag="OHO85_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCQ2673684.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(1183..1893)
                     /gene="narJ"
                     /locus_tag="OHO85_004423"
     CDS             complement(1183..1893)
                     /gene="narJ"
                     /locus_tag="OHO85_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCQ2673685.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1890..3428)
                     /gene="narH"
                     /locus_tag="OHO85_004424"
     CDS             complement(1890..3428)
                     /gene="narH"
                     /locus_tag="OHO85_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCQ2673686.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3425..7168)
                     /locus_tag="OHO85_004425"
     CDS             complement(3425..7168)
                     /locus_tag="OHO85_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCQ2673687.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAAEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         1677 a         2105 c         1940 g         1738 t
ORIGIN      
        1 ccgcaattag atattctgcc catgcgggga aggatgagaa gcttcgacca aggttcgact
       61 cgagcgccag cgagagagcg ttgccgcagg caacgacccg aagggcgaag cgcgcagcgc
      121 tgagtaatcc ttcccccacc accatcactt tcaaaagtcc ctgaactttc aagcgaatcc
      181 gcaattaaat attctgccca tgcggggaag gatgagaagc ttcgactaag gttcgactcg
      241 agcgccagcg agagagcgtt gccgcaggca acgacccgaa gggcgaagcg cgcagcgctg
      301 agtaatcctt cccccaccac catcgcttcc tgaaataatc aaaacctgtt actccgagct
      361 aattttgccc gatggcacca cgtttaccgg acctacaata tcctcccctg taggtcggat
      421 aagcgaagcg catccgacaa ctcaccccgc accgtgccca taaaaaaacc ccgccgaagc
      481 agggtctatg tgaactaaaa ttcgcttagt ggcgagcccg caccagctga tacttacgtg
      541 tcagatactc caccggtacg ctccagatgt gcaccagacg cgagaacggg aacagcagga
      601 ataacgtcat ccccagcacc aggtgcagac ggaagataaa cgccacgcca tcaagatgtt
      661 gagaagcacc accgtggaag gtcactaccg actgcgccca gccaaccagt ttcatcatct
      721 cgctaccgtc catatgctgg gcggagaacg gaatggtcaa caggcccagc gcgcactgga
      781 taacgagcag cgacaggatc aggatatccg ctccggtagt cgttgcacgc acgcgtgggc
      841 tgaacagacg acgcttaagc aacagcacgc cgccaatcag acacagcacg ccgctggcac
      901 caccagcaaa catcgccatt ttctgtttca cttcaatcgg cagccaggct tcatacatcc
      961 agtgcggcgt caacatacca aagaagtgac cgacaaaaat ccccagaata ccgatatgga
     1021 acaggttcga cgccaggttc atccctttgc gatccagcat ctggctggac gccgcgcgcc
     1081 aggtgtactg cccgtagtca taacgcagcc agctaccaat caggaagacc gccccggcta
     1141 tgtacgggta gatatcaaag aagaacatat tcaggaattg cattagtgct gtcctccggt
     1201 ggtgatattc agatattgcg gcgcgacggc accagcaaag cgacgctgat gagcagtgat
     1261 tgctgaatca ccgcagcctt tgtcagcaaa gaatttaacc tgctcttctt cccagacagc
     1321 atccagcgcc tgcggcgtat catcgcgcgc ttcgtcggca attttttccg ccactttgtc
     1381 gctgtcgata gcggtattcg ccagtttcag cagcagatca aacagcacgg cataacggct
     1441 ttcacgctgt tgcagacgcg cgctcagcaa tgccagaatc ggcgcgatat ctttcaaacc
     1501 ttccacggct tcgctttgcg gcagctgcgc caggtactcc agatacagcg gcagatggtc
     1561 cggcaattcg cggctgttta actgcaagcc gtgctgctcg tactgcgcca gcaggtccac
     1621 catcgcctgg ccgcggtcgc gggattcgcc gtgcacatgt tcaaacagca acagtgacgt
     1681 ggcgcggcca cggtcgaaca gttcgctgta ctgggcctgg gcatcgagcg gatccatcgc
     1741 cgttaaatcg cgcaggaaaa tgcccagcgc atgggcatcc tcttttggca gatttttcga
     1801 cgcggcaatc gcctcaaaca tctcttgttg atgctgccat aaggcagcat ccggatattc
     1861 aaggagacgc gatacaatca cgagttcgat catggatgcg gctccgtttt gctggtcaca
     1921 tcgatggcat cgatacgacg gctgttgaac agattgaatt tggtatctga accgtggcag
     1981 ccatcaccaa aggtaaagcc gcagccattt ttctccggga aggcttcccg tgccagttca
     2041 cgatgactac tcggcaccac aaagcgatct tcgtagttag caatcgccag ataacggtac
     2101 atctcctgtg cctgggcttc ggtcagaccg acctcttcca gcgcacgggt atcaacttta
     2161 ccgtcaacgg tttcagcacg tttgtaatga cgcatcgcca gcatacgttt cagcgcgcgc
     2221 agtaccggtt tggtatcacc ggcggtcagc agattcgcca gatactgtac cggaatacgc
     2281 aggctttcga cgtctggcag aatgccgttg ctacccagct cacccgcgtc tgctgcagac
     2341 tgaatcggag acagaggcgg cacgtaccag accatcggca gtgtgcgata ttctggatgc
     2401 agcggcagcg ccagtttcca ttccattgcc attttataaa ccggcgactg ctgtgcagct
     2461 tcaataacgc tcagcggaat accgtcttta atcgcctgct cgatgacttt cggatcgttc
     2521 ggatcgagga acacgtccag ctgacgctgg taaagatctt tctcgttctc ggtgctggct
     2581 gcacgttcaa tcgcgtcggc atcgtataac agcacgccga gataacggat acgaccgaca
     2641 caggtttctg agcacacggt cggctgaccc gcttcaatac gcggatagca gaagatgcac
     2701 ttctcagact taccgctctt ccagttgaag tagatttttt tgtatgggca tccggtgatg
     2761 cacatacgcc agccacggca tttatcctga tcgatcagga cgatgccatc ttcttcacgc
     2821 ttgtaaatcg caccgctcgg gcaggtcgcc acacatgccg ggttcaggca gtgttcgcac
     2881 aggcgcggca aatacatcat gaaggtgttt tcgaactggc tatacatcgc cttctggatg
     2941 ttgtcgaagt tcttgtcttt cgccagtttg tcaaactcac cacccaggtc atcttcccag
     3001 ttcggccctt tttcgatttt cgccatccgt tccccggtaa tcagcgaacg cggacgggca
     3061 atcggctgcg atttgctgcc ttccggcgcg gtatgcaggt tctgataatc aaaatcgaac
     3121 ggctcgtaat aatcgtcgat ccccggcaga tgcgggttag cgaagatttt acccagcagc
     3181 atggcgcggt tacccatgcg cggctgcagt ttgccattga ttttacggat ccagccgcct
     3241 ttgtattttt cctggttttc ccagtcagtc gggaagccct gacccggctt ggtttccacg
     3301 ttgttgaacc acgcgtattc cacgccttca cggctggtcc agacgttttt acaggtaact
     3361 gaacaggtgt ggcagccgat gcacttatcg agattcagca ccatgccgac ttgtgaacga
     3421 attttcattt tacgctctcc tgtacctggt cattgccttc gccatctaac cagtcaatgt
     3481 tcttcatctt acgcactaca acaaactcat cgcggttaga acctacggtg ccgtagtagt
     3541 taaagccgta tgccagatgg gcatagccgc cgatcatatg cgtcggtttc ggcgtgatac
     3601 gggtgaccga gttatggata ccaccacgct gttgggtaat ttccgaacca ggcaggttaa
     3661 cgatacgttc ctgcgcgtgg tacatcatgg tcatccctgc cggaacacgc tggctgacaa
     3721 ccgcacgggc agtcagagca ccgttgctgt tgaatacttc aatccagtcg ttatcggcga
     3781 tacccagatc tttggcatcg gcttcactca accagaccac cggaccaccg cgacctaaag
     3841 tcagcatcag caggttgtcg ctgtaggtgg agtggatacc ccacttctgg tgcggcgtca
     3901 ggaagttgag cgctttttcc gggttgccgt tggatttctg gcctatcact tctttcaccg
     3961 aacgggtgtc gatcggcgga cgataaacca gcaggctttc accgaaatca cgcatccact
     4021 ggtgatcctg atacagttgc tgacgaccag agagcgtacg ccatgggatc agctcgtgaa
     4081 cgttggtgta accggcgttg taagaaacgt gttcatcttc cagaccagac caggtcgggc
     4141 tggagataat tttgcgcggc tgtgcctgaa tatcgcggaa gcggatcttc tcgtcttctt
     4201 tattcagcgc cagatgcgta tggtcacgac cggtaaattc gctcagggca gcccaggctt
     4261 tcacggctac ctgaccgttg gtttccggtg ccagtgtcag gatcatctct gccgcatcaa
     4321 ttgcggtgtt cagcatcggc tggcctttcg ccggaccttc cgctttggtg tagttgagct
     4381 tacgcagcag atccatctcg ctctgggtgt tccaggcaat ccctttaccg ccattaccga
     4441 ttttctccat cagcgggccg atagaggtaa agcgttcgta agtcgccgga taatcgcgct
     4501 ctacgaccat aatgtgtggc gcggttttac ctgggatcag gtcgcactcg ccttttttcc
     4561 agtctttcac atccagcggc tgcgccagtt cagcggcaga gtcatgctgg ataggcagcg
     4621 tgacgatgtc ggtttcttta cccagatggc cgacgcacac ttcggagaat ttcttcgcga
     4681 tggctttgta gatttcccag tcgcttttcg cttcccaggc cggatcgacc gccgcagaca
     4741 gcgggtgaat aaacggatgc atatccgaag tattcatgtc gtctttttcg taccaggtcg
     4801 ccgtcggcaa aatgatgtcg gaatagagac aggtgctcga cagacggaag tccagcgtaa
     4861 ccaccagatc cagcttgcct tccagaccat tgtcctgcca gtccacttct tccggcttca
     4921 cgccgccctg ttgccccaga tctttaccct ggataccgtg ctccgtcccc agcaggtact
     4981 tgagcataaa ctcatgacct ttaccggaag aaccgagcag gttagaacgc cagatgaaca
     5041 ggttgcgcgg gtggttttta ccgttttctg gttgttctgc cgcaaaacgg atggaaccct
     5101 ctttcaggga tttcaccgta tagtccaccg gattcatccc ggctttttcc gcttcggcag
     5161 cgatagtcag cgggttagtg cctaactgcg gtgcagacgg cagccagccc atgcgttccg
     5221 cacggacgtt aaagtcgatc aggtgtccgg tataacggga tttatccgcc atcggtgaca
     5281 gcaactcttc cgccgtgacg gtttcataac gccactggct ggagtggtta tagaaataag
     5341 aagtgctgtt catgtgacgc gccggacgct gccagtcaag ggcaaacgcc agcggctgcc
     5401 agccggtttg tggacgcagt ttttcctgac ctacatagtg cgcccagccg cccccgctct
     5461 gaccgacaca gccgcagaaa atcagcatgt tgatcagacc gcgatagttc atgtccaggt
     5521 gataccagtg attcagcccc gcaccgacga taatcatcga acgaccgtgc gttttatcag
     5581 cgttatcggc aaattcacgg gcgatgcgaa taatctggct gcgagaaacg ccggtaatct
     5641 gctcggccca ggccggggta taagctttca catcgtcata gctggttgca cagttaacgt
     5701 cgttcagacc acgttccaga ccgtagtttg ccagcgtcag atcataaacg gtggtcacca
     5761 gggcggtgct gccatcagcc agttgcaggc gtttcaccgg cagtttgtgc agcagcacgt
     5821 tttccagttc cactttgttg aagtgttccg tgccgtcgcc accaaagtac gggaagccta
     5881 cctcagcgat ctcatcctgg ctacccagca ggctcagttg caactcggtt tcttcgccag
     5941 ttttgccatc gcgctgctca agattccatt tgcccttctc gccccagcgg aagccaatag
     6001 aaccgttcgg cgcaaccatt tcgccattgg tattaaaggc gacagttttc cattccggat
     6061 tgttttcctg gcccagcgca tcaaccagat cagcagcgcg cagcatacga cctgcagcgt
     6121 agtaaccgtc gcgttcttcc agcatcacca gcatcggcat gtcggtgtag cgacgcacat
     6181 agtcggtgaa atactggctt gggttgtcga ggtggaattc acgcagcatt acgtggccca
     6241 tcgccagcgc cattgctgca tcggtgccct gtttcggtgc cagccacaga tcgcacagtt
     6301 tggcgatttc agcgtagtct ggtgtgacgg caacagtttt ggtgcctttg taacgcactt
     6361 cagtaaagaa gtgagcatcc ggggtacgcg tctgcggcac gtttgagccc caggcgatga
     6421 tgtagctgga gttgtaccag tcagcagatt ccggtacgtc agtttgctcg ccccaggttt
     6481 gcggagacgc aggaggcagg tcgcagtacc agtcgtagaa gcttaagcaa gtaccgccaa
     6541 tcagcgagag atagcgtgca cccgatgcgt aagaaaccat cgacattgcc ggaattggcg
     6601 agaaaccagc aacacggtcc gggccgtagt ttttgatggt gtaaacgtta gatgcggcga
     6661 tcagttcgtt cacctcctgc caggaagaac gaacaaaacc accgcgtcca cgcgcctgct
     6721 taaagctttt cgctttatcg gcgtcttcaa tgatagaagc ccatgcctca accggatcgc
     6781 tatgcagcgc cttcgcttca cgccacattt tcatcaggcg tttgcgcatc atcgggtatt
     6841 tcaggcggtt ggcactgtaa agataccagg agtagctggc accgcgcggg cagccgcgag
     6901 gttcatggtt tggcagatcc ggacgggtac gcggatagtc agtctgctgg gtttcccagg
     6961 tgaccagacc gtttttgacg tagattttcc agctgcagga gccggtgcag tttaccccgt
     7021 gggtagagcg gacgattttg tcatgctgcc aacgctggcg atatccatcc tcccagtcac
     7081 ggttggtatt gagaagctgg ccatgcccat cggcaaaggt ttcacccttc tgtttgaagt
     7141 agcgaaaccg gtccaggaat ttactcatcg gttttctcct gtgggagcct gtcggccctc
     7201 tgataaatcg acattgctaa tgtttaaggg caaggtaacg ctctgaaaca gcgtgggaat
     7261 tgataacgat caagagtgat ggggatgaaa aataaagtaa attcctaaga taccccttta
     7321 ggataattaa cagccacagc ataacagact gtaaattaag acgttttaca cctgtagtta
     7381 tttaaactat taagaatttt atgcaggtgg gtattaagga gtattcccca tgggtaacat
     7441 attaatggct cttaatgaaa
//
LOCUS       DAJOWB010000182         7400 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJOWB010000182 DAJOWB010000000
VERSION     DAJOWB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.182"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            988..1335
                     /locus_tag="OHO85_004426"
     CDS             988..1335
                     /locus_tag="OHO85_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCQ2673688.1"
                     /translation="MILDSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFI
                     GSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEI
                     GAVPAIPQQSSLF"
     gene            1388..1783
                     /locus_tag="OHO85_004427"
     CDS             1388..1783
                     /locus_tag="OHO85_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCQ2673689.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1824..2567
                     /gene="cmoA"
                     /locus_tag="OHO85_004428"
     CDS             1824..2567
                     /gene="cmoA"
                     /locus_tag="OHO85_004428"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCQ2673690.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HNAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            2564..3535
                     /gene="cmoB"
                     /locus_tag="OHO85_004429"
     CDS             2564..3535
                     /gene="cmoB"
                     /locus_tag="OHO85_004429"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCQ2673691.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(3700..6129)
                     /gene="torZ"
                     /locus_tag="OHO85_004430"
     CDS             complement(3700..6129)
                     /gene="torZ"
                     /locus_tag="OHO85_004430"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCQ2673692.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFDDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGHASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVALQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAIADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(6154..7254)
                     /locus_tag="OHO85_004431"
     CDS             complement(6154..7254)
                     /locus_tag="OHO85_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCQ2673693.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKDSET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSHIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         1775 a         1853 c         1692 g         2080 t
ORIGIN      
        1 tactttaaag atatctgtat taaatctgta cagtaacttt tcagactctg aaaattttta
       61 taaaatcgta gtagcccctc ttcaaattcc ctctctgaga atcttcaaaa atatctgaca
      121 gatgtctgta gctgctaagt acccgtcaca ccagctatgt gacccccttt gcagaatttt
      181 aaaatatcta tgaggtttat tcgataaacc gaatcaacta ttgactttct tcatagcatc
      241 tgttaactcc acctcaagaa accttcacca cacttcacgg catctatcaa gaagatttca
      301 cctatcaata tctgtaacat tttatagttc tgtgaagtcc tccccagttt tctctgtaac
      361 agctacaaac ttccgtgacc ttatgtatca atgacttaca agacatgctc tcaaggtgct
      421 gtatagcctc ttatcactca atacacaaaa cccttataaa tcagtactga tacatataca
      481 gtgtgttttt aacataatag ctgttttacg cactaatcgt gttctacatg aaatctgtat
      541 agatattaag gtgtctgtaa gtgatttttt aagaagggac tacgaagatt tgacaaggta
      601 actataacca tcactttcta actttcacat agctgattgt tatctcttgc aactgtaacg
      661 tatggtgacc aactatctat atatcgttat atagccctgt atatagcact atatgtagtg
      721 tctggagagg gacgactaac acgacacagt ttaccctgaa aagagacttc acagattctt
      781 tatatctatc aataacttag gttactaagg ggtgatgtag gggcttggaa gatactgcat
      841 agatactgtg gaaaattgct tagatacttg agaatttacc gttaaattgc atagtcattc
      901 acctgatatt gtcctgtttt tacaggtatg ttctcatagt tgattcggat gttctaggaa
      961 tcatcctcat agtgtattta gaccttgatg attttagaca gccgcccggt tcatgcagca
     1021 cgtccacaca gtgaagctat tcgcgacgct caacgaaaaa aacctaaagt tccggtacat
     1081 gctgtactga cggcgacaaa tccgctgatc cgttttatcg gtagtgatga tatgacgcaa
     1141 aaccgtgaat tatttcaggt ctggttacaa aaattagcgc agtggcatca gaccactacg
     1201 ccttatcttt ttttacatac gccagatatt gcccaggccc cggaactggt acataccctg
     1261 tgggaagact tacgtaaaac gcttccagag atcggagcag ttccggctat tccacagcaa
     1321 tcttctcttt tctgaatttg ccacctatca tagacaagtg ccatcggcca ttttaaaggg
     1381 agtttgtatg gtaagcgcgc tgtatgccgt tttaagtgcg ttgttattaa tgaagttctc
     1441 ttttgatgtc gttcgcctgc gaatgcagta ccgcgttgcc tatggtgacg gtggttttag
     1501 cgaactgcaa agcgctattc gcattcatgg taacgcggtg gaatatattc ccatcgcgat
     1561 tgtgctgatg ctgtttatgg aaatgaatgg cgcagaaacc tggatggtgc atatttgcgg
     1621 catcgttttg cttgctggtc gtctgatgca ttattacggt tttcatcacc gtctgtttcg
     1681 ctggcgacgt tccggcatga gcgccacctg gtgtgcgctg ttgctgatgg tgctggcgaa
     1741 tctttggtat atgccctggg agttggtttt ctccctgcgt tagcgcacaa tacgccactt
     1801 tctttttccc ggatttttac gttatgtctc accgcgacac gctattttct gcccctatcg
     1861 ccagactggg cgactggacc tttgatgaac gggtagctga agtcttcccg gatatgatcc
     1921 agcgttccgt tcccggctat tccaatatta tttccatgat tggtatgtta gccgagcgct
     1981 tcgttcaacc tggtacgcag gtttacgatc tgggttgttc tctgggcgcg gcgacgctct
     2041 cggtgcgtcg caacattcat catgataatt gcaaaattat tgccatcgac aactccccgg
     2101 cgatgattga acgctgccgt cgtcatattg acgcctataa agcccctacg ccagtagacg
     2161 tcattgaagg tgatattcgc gatatcgcca ttgaaaacgc atcgatggtg gtgctgaatt
     2221 ttaccctgca attcctggaa ccttccgagc gccaggcgtt actggataaa atttatcaag
     2281 ggctgaaccc cggcggcgcg ctggtgcttt cggaaaaatt cagtttcgaa gatgccaaag
     2341 ttggtgaact gttgttcaac atgcaccacg actttaaacg tgccaacggt tacagcgaac
     2401 tggagatcag ccagaagcgc agcatgctgg aaaacgtgat gctgaccgat tccgtggaaa
     2461 cccataaagc acgcctgcat aacgccggtt ttgagcatag tgagctgtgg ttccagtgct
     2521 ttaactttgg ttcactggtg gcattaaaag cagaggacgc tgcatgatcg actttggtaa
     2581 cttttattct ctgattgcca aaaatcatct ttcacactgg ctcgaaacgc tgcccgcgca
     2641 gattgctaac tggcagcgcg agcagcagca cgggttgttt aagcagtggt caaatgcggt
     2701 ggagtttcta cctgaaatta aaccgtatcg tcttgattta ttgcatagcg ttacggcaga
     2761 aagcgaagag ccactgagcg cagggcaaat taaacgcatt gaaacgctga tgcgcaacct
     2821 gatgccgtgg cgcaaagggc cgttctcact gtatggcgtc aacatcgata ccgaatggcg
     2881 ttccgactgg aaatgggatc gtgttctgcc ccatctttct gatttaaccg gacgcaccat
     2941 tctggatgtc ggctgcggca gcggttatca catgtggcgc atgattggcg caggcgcgca
     3001 cctcgcggtg gggatcgacc ctacgcaact gtttctgtgc cagtttgaag cggtgcgtaa
     3061 actactgggt aatgatcagc gcgcgcattt gttaccgtta ggtattgaac aacttccggc
     3121 actgaaagcc tttgataccg tcttttcgat gggcgtgctt taccatcgcc gttcaccgct
     3181 ggagcatcta tggcagttga aagatcaact ggtcaatgaa ggtgaactgg tgctggaaac
     3241 gctggttatt gatggtgatg agaacacggt gctggtacca ggcgatcgtt acgcgcaaat
     3301 gcgtaatgtc tatttcatcc cttccgcgct ggcgctgaaa aactggctga agaagtgtgg
     3361 ttttgtagat atccgcattg cggatgtgag cgttaccacc acagaagagc agcgacgcac
     3421 cgaatggatg gttaccgaat cgctggccga tttcctcgac ccacatgatc cgggtaaaac
     3481 ggtggaaggt tatccggcac ctaaacgcgc ggtgctgatt gcgcgcaagc cgtaaaggtc
     3541 tggtattact gccggatgcg gcgtgaacgc cttatccggc ctacaaaacc ttgctaattc
     3601 aatatattgc agggactatg taggcctgat aagcatagcg catcaggcag ctttacgttt
     3661 gcataacctc agcgcccgtt tccgggcgct attcacgtct tacgcctgaa cagctggctg
     3721 atcaaacgcc gttaacttcg gtgcattgcc cgtatatttt tcgatataca ccaacgctga
     3781 gttaccggca caggcatttg ccagctgcga gctggggata tccgccgtta acacgttcgc
     3841 actgccgttt ttacacaagc cattttccag atctggccat gcaccttcat gcacgcatac
     3901 cacgcctttt ttgatcccgt cagtcaccac cgcgcctgtc aaaatctgac cacgtttgtt
     3961 ccagacacgc accagatcgc cattcgcaat accaaagcgt gcagcatctt cggtgtgaat
     4021 agtgattggt tcacgatcgg cgatcgcata ttttttacgc agttccgcat agttaagctg
     4081 actgtgtaaa cggtgtgccg gatgtgcggt cagaagctgt aactgcttct cgtcggcggt
     4141 acctttccat tcatcaggag ccagccaggt tgggtgtgcc gggcaatcct tatagccaaa
     4201 tttttccaac gtttttgaat agatttcaat tttgccgctt ggcgtaccca gcgcattttt
     4261 caccggatcg gcgcggaaat caccataacg aacgtactgt tcgttcttct cgctgcggcg
     4321 catttcgatc agtttatttt gctgccagaa ggcattaaac attggcatag tgacgcgttg
     4381 cgcacgggca cctttctgag cggcatcata gaaaaatttc agccacgcca tttcatcttt
     4441 accttcggta tagatctctt ttccgccagg tttgagtaat tccgcaagat cggcaaacac
     4501 gtcaaaatcg ttacgcgctt caaattgcag agcaacagcc tgcttcatcg gcacaatatg
     4561 ctggttgctg taatcaccgg tcatcgtcaa gtcattgcgc tcaaacgatg tggtgatcgg
     4621 taatacgata tctgcgtgtt tggctgctgc ggtccagtag cattcagaaa cgacgatcat
     4681 ctccggtttc tgccatgctt taatcagacg attagtatcc tggtgatggg taaagttacc
     4741 gccacccgcc caccagatca ttttgatatt cggatacgtt tgttctttac cgttatgctg
     4801 atatttgccg ccaggatttt ccagcgcatc gacgatacgg gcaacaggaa tcgccgtcat
     4861 tccgccatca tcagcggcct cgctggcatg tccggcaatc gccgcagaca tttccggcag
     4921 cacgccgcca acacgcgtcg ggttgccacc gttggagtaa tgataagaga aaccaaaacc
     4981 gccgcccggt gtaccaatct gccctaacat cgccgccagc gtcaccagca tccagtgttt
     5041 ctgttcacca tattgctggc gctgaattcc ccagcctgcc atcagcattg tgcgattcgc
     5101 cgccatcagt tcagcgagtt tgactatctg cgcttccggt acgccagtaa tttccgccgc
     5161 ccagaccgcg ctcttcggcg tattatcgct cttacctgtc agatactctt caaactgcgg
     5221 atacccggta gtgtattttt caaggaacac tttatcgtgt ttgccttgtg tcatcagggt
     5281 atgcgcaatc cctaacatca gtgccacgtc ggtgcccata ttcggcgcga tccaggtggc
     5341 attatcgtca aagaattcga tggtttcgga gcggatagga tcaatggcaa tcactggttt
     5401 gccagatttt ttcagctgat ggaagtattc cagcccttgc tcatcggtac tgctccaggc
     5461 aatttttaag gtattcagcg ggttcattcc ccacagcacc acaacctggc tgttttccag
     5521 aatcagcggc caggaggtct gctgttcata cacctctaca gaaccgacca catgcggcat
     5581 gatcacctgt gctgccccgg ttgaataatc gccgctatgc ccggaataac cgcccgccag
     5641 gttcatataa cgttgcagta aggtttgcgc tttatgcaac acgccagaag agcgccagcc
     5701 gtaagatccg gcaaaaatgg ccgatggtcc gttagcttta cgaatacgat catgttgctc
     5761 atgaatcagt tttaatgcct gttcccagct cacctgtaca taggtatctt cgccacgacc
     5821 tttcgccggt tgcagtggat tatcgagata gctttttctc accatcggat gctgaatacg
     5881 cgccgtggtg tgtacctgat ccgccgccgt agactgtaag gaattcggta tggttttcgc
     5941 cagcgcgcct gttgaagaaa caatcttgcc gtccttcact tctacgttca tcgctcccca
     6001 acgtcccgcg gtgaggattt taccgccctt ctcttctgcc catgcgggga gcggtgctgc
     6061 cgatgtcacc accagcgctc cagcggcaat accgctgtgt ttaataaatt cacgtcttgt
     6121 taatgtcata acttcctccc tgatcaacga ggatcactgt ttctcggtaa tatctttggc
     6181 gttgtactgg aaataccgcg ttaaaatgtc cagttcgttt tcgctcatgc tggttcgtgc
     6241 ccccattcct ttggcaatgg acggccacgc attgacggtg taatggtcgg cggcaatagg
     6301 ggcatgacaa ccagcgcaat aggtatcgtc aagtttttca gcgtattgcc atagcggttt
     6361 acggtccgct aatgcgggat cggtaagcgc accctgtaaa gacgcctgac gccattgatt
     6421 gccgtattcg tcagcctgcc attccccgtt tacagtgagc gccttgatac cttcttcact
     6481 taatgtggct agcgccagcc gttgacctgc cgccaggtag agcgtgtttt cactgccctg
     6541 catttgataa ccctgcaaca gaacgatcgg ctgtttgcca ctggcatcaa cgacggtgag
     6601 atcggttcca ggattcacgg tagccagctc gcctatgtga gaagttttga aaggataaat
     6661 atgtgcgcca ttagtaactg aagtagcggc ctgactttcc agctcatgcg ccgcgttgtc
     6721 atccattttt atttctggcg gaaaatgggc aatgccttta tgacaatcga tacaggtttc
     6781 gctgtccttt tgtgctttgt tatgcatttt ctgcgcagat tcactttgcg aggcaatatc
     6841 catggcatca aaagaatggc aactacggca cgttgcagag tcagtggctt ttaattcttt
     6901 ccatactgtt tcggccattt cctggcgatg agcttcgaac ttatcgtcac tgtctatttt
     6961 gccgctaaca aattcatgat aaatatcttt agatgccttt aatttagcaa ataaataatc
     7021 catccctgac tttggaatat ggcaatcggc acattccgca cgtatccctt tctggttcga
     7081 aaagtggaca gttccctgat attcctcaaa aggtttactc atcgagtggc aggaaaggca
     7141 aaatgctgta tccgacgttt tatgtaaggc tttttgcgcc agcaataacc cgccgccacc
     7201 aaccacaacc gctatcagca aaaacaataa cccaatgcgt tttttccctc gcatagattc
     7261 ctctaattaa attactggac accgaaatca ttgtataatt aaatatacaa cgaattaacc
     7321 gcttgcataa ttaggcacaa cactgcctga aacaatcgat aaagaatatg atttattaca
     7381 atgtaatcat taattgctaa
//
LOCUS       DAJOWB010000183         7341 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJOWB010000183 DAJOWB010000000
VERSION     DAJOWB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.183"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(43..1053)
                     /gene="pyrD"
                     /locus_tag="OHO85_004432"
     CDS             complement(43..1053)
                     /gene="pyrD"
                     /locus_tag="OHO85_004432"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCQ2673694.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(1164..1901)
                     /locus_tag="OHO85_004433"
     CDS             complement(1164..1901)
                     /locus_tag="OHO85_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCQ2673695.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAIRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTWNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(1867..2382)
                     /locus_tag="OHO85_004434"
     CDS             complement(1867..2382)
                     /locus_tag="OHO85_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2673696.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(2390..2932)
                     /locus_tag="OHO85_004435"
     CDS             complement(2390..2932)
                     /locus_tag="OHO85_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2673697.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(2944..4014)
                     /locus_tag="OHO85_004436"
     CDS             complement(2944..4014)
                     /locus_tag="OHO85_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2673698.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(4005..6605)
                     /locus_tag="OHO85_004437"
     CDS             complement(4005..6605)
                     /locus_tag="OHO85_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCQ2673699.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVCDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            complement(6630..7331)
                     /locus_tag="OHO85_004438"
     CDS             complement(6630..7331)
                     /locus_tag="OHO85_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCQ2673700.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
BASE COUNT         1868 a         1875 c         1641 g         1957 t
ORIGIN      
        1 taaagccctg attatcttat aaagaggaat tcgaagaaat aattagatat gggtaacgat
       61 ttctttaatc agcggcggac ctttaaaaat aaaaccagaa taaatttgca ccagtgaggc
      121 acccgcagca atcttttcac gcgcagcgat aaccgagtcg atgccgccaa caccgatgat
      181 cggtaagcga ccgtttaatt ccagtgacaa gcggcgaata atttcggtgc tttttaactg
      241 aagcggacga ccacttaagc caccggtttg atcgcaattt ttcattccct gaacaagaga
      301 acgatcgagt gtggtattgg ttgcaataac gccatcaata ttatggcgaa ctaaactatc
      361 ggcaacctgg atcaattctt cttcagaaag atccggcgcg atcttcactg cgatcggcac
      421 atatttatgg tgcatcgctt gcaaatcatt ttgcttattt ttaatcgcgg ttaagagatc
      481 atccagcgct tcaccatatt gcagtgtgcg taatcctggg gtattcggcg atgaaatatt
      541 gatggcgata tatcccgcat aggcatagat tttttccata caaatcagat agtcatcttt
      601 gccctgctcc actggcgtat ctttattttt gccgatgtta atacccagga cgccgtcata
      661 atgggccttt tttacgttct ctacgaggtt atcaacgcca agattattaa agcccatacg
      721 gttgatcaaa ccttcggcat ctaccagacg aaagagacgc ggcttgtcat tacctggctg
      781 tggacgtggc gtgacggtac cgatctcgat cgatccaaat cccatcgcgc ctaacgcgtc
      841 aatgcactcc ccgtctttat caagaccggc tgccagacca agcggatttt taaacgtcag
      901 gcccatgcag ttaacaggtt tcgcaggcac tttctgccgc accagtgctt caaacggcgt
      961 tcctgtaata cggcgtaatt gctgaaaagt aaactcatga gcgcgctctg gatcgagctg
     1021 gaaaagggct ttacgaacga aggggtagta catgaactct cctggattcc cggtgtgcaa
     1081 accgggggcg tattatgtgc gagttcggac caaaagggaa ttgacctgcg gcaaaaaaaa
     1141 cgcaaacgtt ttccgaacct gtttcagggc agattttgct ccttctttgc actcaacccg
     1201 ccgtaatcat ttatcgcccg ccaggcgaca tgacacggtg cgattgcctg acaccagctt
     1261 gtctgacgct ggcttttagg cggcacaact cccgcattac ttaacgcttt accatttacg
     1321 ctcacctgaa tcaggttaat gtagtaaggc gttgggttag tgagttgtac accctgcgaa
     1381 ttccatgtcc agcgtaattg ttgataagct tccagcgggt cgcccggcaa accttcgggt
     1441 cgccagaaca gtttaaatac cgagcgtatc gccactttta cgctctgatt ttcaactttg
     1501 ccggatggca cgctggcaat gcttaactca aatagcgttt cgcgatccac aggcaagatg
     1561 tcgctttccg ttctcagcaa gcgcaacgtg cctgtagtac cgggcttcag gagaataagt
     1621 ggcggagcgg ccagtaatgg tgctggcact gtcgacgctt cccccccggc ccaacgcgtt
     1681 gggcggttga ttttgctgtt aataagccag gattcctgac tggtattagt cagtaaaata
     1741 gatatcgatt ctctgtctgc cggaaagata aatcgcgttc cgccgaccac cactcctgcc
     1801 tgcaattcac ccgccaccag cagcgataaa acggcgaaaa tcaagagcgc caatctgttc
     1861 cacgtattag tcataatgaa gtacaaatgt cgcgctggca ttcactttac ccgactgcac
     1921 atcgctattg acaggtcgca attgcgcata aaaagttaat ggcaccttac cgctatgtag
     1981 gctgtagtct ttactcgcct ggttaagcgg aatcaatatc ttcgcatcat ccagaatcgc
     2041 aattgccagc ccgctggctg catccgtacc actttcaatg gacagcattt cgctctgcac
     2101 tccgtcgggg gttcctgaaa aggtgacgtc aaccgtgctg acatcagttc cgcaatcctg
     2161 caaattaatg atgaaagatt tctccgggcc ataattccca tgagaaaaac gtgtcagcga
     2221 aacatcgcct aactgcacat tgatttgctt gctatcctgt gccagcacgc atgtattacg
     2281 gcgaaacgat ccgctaatgt tgaccgtgcc atcatgggca aatagctcac agccccccgt
     2341 taatcccagt aagaacagaa tccagattat ccgtttcaac atcactcctt cactggtaaa
     2401 ccagatcaaa atagagaacc gccgtcgcat taccgcccgt tgctcccgcc tttgtggact
     2461 tataacgaag ttgatatttg agtgtgttat ccccggcttt taagggcgta agaggctgga
     2521 cctgattgag cgggatttct tgctgacttt gcgcatcaag aatttgcacg gcaatccccg
     2581 ttgccatacc accgcttccg cctgtgtcag ttagtttgag cagtgtcggc gcaagggtgt
     2641 cagcctcgcc gctaaaaagg acgtccgcgc cagtaatacc agcggcacaa ccggtgatat
     2701 cgatattcaa atcagcagca gtgcttacgg tattcgcggc ctgaaaatca ctgatattaa
     2761 aatcaccaat atgaatattt tgtaatgcac ttttggtggc gacatcacag ccctgatcga
     2821 ccacattccc ataaatatgc atactaactt gttcaggccc agccatcccg acatggatgt
     2881 taagaatgct aaagaacaag ataacgcact gaaatatcgt ttttttcttc atacgttcac
     2941 gcgttaatca aaatttattc tcaccgttgc cgtggcttca aatcgcccgg tttctggtgg
     3001 tacacctgtc gtgctgactg gatagacatt cattcgcgcg atactgtctg catcatcagg
     3061 aatagagagc ggtaatgttc caccagcagg ggaaataatg ttgttctggc tatcggtcac
     3121 caccacgccc acgccaggac gtgacgtctt aatcgcctgg ggatagctgg ggtctgtggt
     3181 tgccgtcaga ctcaggttga cgttaactaa ccccgcgttt cccgtgttac acctgactgg
     3241 aattgcaagt tctgccgttc tggccccaag aggcttatgc cctacaacac gaaagttagc
     3301 cgcaaatacg tcgcccagat cgacctcgat tgtttctccg gcattgacgg aacatgagcc
     3361 aagtgaattg atcgtaccag acaggtaata cactaaaacc ggtgtacctg tagtcgtgca
     3421 ggaatcacct gcgggtatgt tgtagcattc aaataatcgc gccagcgcca taggaggaat
     3481 gacgaccgaa ccaacgaacg gctgcaatat tttcagggaa aagctaccgc tattaccgct
     3541 agccccctgg ccacctaaac gttgaccgcc gggatcgcaa atatgccatc gtgtatctgc
     3601 ccctgtgccc acgttttcga aaggaaccgc agtaggagaa ctgcctttaa cctgcaataa
     3661 aactttggcg gcaagatagt cattaatttt gtaccagttg tcgcctaaat agactaacgg
     3721 tgaatccgcg gcgaatgccc atcggccact tctgacattg tctttatcgc aatcacagga
     3781 aacataataa tcattcgatt cgttccactg ataaaatttg tctttaacta ctccagccgt
     3841 attttcttct tcctccaggg ttttaacgcc aaaattgacg ccaatatacg gattcccctg
     3901 agtggtatga cactgcccgc tataagcagc agtttgagcg caccatagca ttaagacggc
     3961 ggcaaaaaat agtcgtagta ataacacgca tttttctcca aagattatct gcatatggca
     4021 tccattcgcg taatcgcaga gttaatttgt tgttcggtaa gctggtaact gatatgacaa
     4081 atttgatcgg gagcttcgcc ccagcgtaca gttatcgctc cctgagcggg caaaccggac
     4141 atatagagcg agccatcatc atcaataatg ccgctattag tctgtatgcc gtccagcgtc
     4201 gctatcgcgc caaaacgcag cggtataccc tgctttgatg ttttcattaa tacccttgct
     4261 cccgcatggg cattgaactc cgccagaacc aacgcccctt tggtgggaac cacgttgact
     4321 actgcatttt caagatccac gttacgtttt aacgatgccg catcaagtgc gattcgattt
     4381 tcccgataag aagtggcata ggggacaacg gtatatccgc gccagtcagt tttcagcccg
     4441 gtgctgttcg ccacacgagt attctctgcg cctggtgctg caatcagcac gttagtttcc
     4501 cccagcgatt gccccagggt aatgccctgt gaatgggcaa ctaaactgcc tgaaagagcg
     4561 tagttcagtt gttgttgact gccgttatcg ctgtagttgt agcccactcg ggcatctgca
     4621 aacgcccctt tgtagtccat gctggcgcta ccattagccg ttttcccctc gctgttatat
     4681 ccctgttgca cgctgtagct caggttaccg tcatcgagta gtgtctccgt taaacccagg
     4741 ttttgtgtaa tgtgcccttc gttatcgata ctggtgttag aggtcatcca ggcgtaattt
     4801 tttttcctgg tatagctatc attacttctg gggagcaaca gattgagcgg cagggaaaaa
     4861 tttagtgcaa acacctgatc agcatcaggt tgaccacggg acttactgta gttccaggaa
     4921 acccccagcg agagatcttt aatttgcgtg ttgtagccaa actgtaatag ccgatcctgt
     4981 tgatcggtat gccagtaagt ttgctggcta ccacttaaat aaaacgaacc gtactcgcct
     5041 aattgctggg agatattgac ctgcagtttg ccacgtttgg tgtaaaacaa attgtaataa
     5101 cgcgaccaca tcggcgtatc ttcatcatca ccgtcattaa attcgtagcc atccatatgt
     5161 ttatacatgg tgtcggaaag ggtgtagaaa cccgacgtcg aatagcgata gcccatcaat
     5221 tgaaaattag tgcccaattg gttgagcgtt ttggcataca gaaaacgata tgactgacct
     5281 ttatgttcgc tttggtctgc cagcgtactc ttcgcctggg tcgcatcgaa cgaaattgct
     5341 ccgaaatcgc caaggttaaa tcccagacca aacatggcgg cacggtaata ttcagcatat
     5401 tgtccaccac cataccatgt cgttccccac ggtccgcccc actgcaacgt ggcctgggca
     5461 aatttgctct cctgctgttc attactattg gttctgtatt tcgccagtgt caccgcgtat
     5521 ttgattcgcc cctgacgctg gagtaatggc acgctggaaa acggtacgct gtagctattg
     5581 acgctgccgt ccgcttcttt gatttcaact aacaaatcac cgctcgacga cgtggaatag
     5641 agatcactaa tttcaaaagc accaggcgat acataagttt ggtaaatggt gtaaccattt
     5701 tgcttaatcg ttatctgggc attgcttttg gcgataccac gcaccacagg cgcaaaccct
     5761 ttcaggctgt ctggcagcat gttgtcatct gacgccagct gcacaccacg aaagctgaca
     5821 ctatcgaaaa aatcgcctgc agtataatca tcacatacgg tcagttcgcc ttttagtgga
     5881 ataaccgccc gctgcaaata gctgctgaga ttcttccatt ctgcggtttg gccactactg
     5941 cgactccatg ttgaattgtt gcgcaatctc catgggccta aattaacgcc actgtttaaa
     6001 ttcagaaaat agctgtcgcc agaatcactg tctgcgctgc tatgaatact gttagccccg
     6061 ctaaacgaat atcccagtaa tagcgcgttg atcccttcat cccaacgttc aggggggaca
     6121 taaccacggg cattactttt gatggcgatt tgcggtactg aaatttccag acggagttta
     6181 ttcacattaa acgttacgct ggcatccgga ataatctccg ccaatggcac acacgcttgt
     6241 ttgtccagtt gtttaaaagc cggaaacgca tcagtattaa cacccattgc atccaggctt
     6301 tcagtcgtca aacaggcggc aatccccttc tctggcgtta tctcaacaaa attgacgttg
     6361 cgcgtatcta caattgtctg attcagaaca atatcgacac gataaacccc tggcgttatt
     6421 ttttgtcctt tttcgaaacg agacaaatcg gccaccattg agggatcatc tttgaaaaaa
     6481 gcaggatcga accaggtttc agcttgtgca ttgaacgctg ccgacacaaa caccaccagc
     6541 ccaccaataa acgatggcac tccaccagag cttaacaggc tgtgttgtcg atgagttcta
     6601 tacatctcgc tctcttaact gaagacggct tacgatttac agttgagttg cggcgtttcc
     6661 gccccgtagt cattgatggt tcgatacgtt acgctgctat ttttggcccc ctttggcaga
     6721 gaaaattggt atttatcaaa gggggaaatc atttgatttt taactacttt tccacctatt
     6781 ttcaggtctg tcagggtaat gaaataaggt gttgggttac tgaccgtaac catgtcagct
     6841 gacgaagtgc aacgcagttt cgccggagcc tcgcctaagg gatacgctaa accttttggt
     6901 cgccagaaaa gtttgatgac gctttgtgtg gcaatcttca gggtatttcc ctcttcccgc
     6961 ttcgttgttg aagggactgc ccgtacgcta gtgaagaaaa gggtttctct gtctttcgcc
     7021 aacggcgcac caatgtacat aatgcgtaac agattctcat tagccggatt cagcacaaat
     7081 agcggtggtg taataataaa atctgctgat tttttattat ctggggtcat cacccatgac
     7141 tgaataagat aaggaacatc cggactggtg ttacgtacac ccagtgaaat ttgctcttca
     7201 ccttctgaat aaacaagacg tgttgctgca agtccaatcc cgcctttacc tgccgcccat
     7261 gcggatgagc agctgagtaa tattgccgtt aaacttacgg tcacaattcc ctttgttatg
     7321 caagttttca taaatagtca c
//
LOCUS       DAJOWB010000184         7303 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJOWB010000184 DAJOWB010000000
VERSION     DAJOWB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.184"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(303..1439)
                     /locus_tag="OHO85_004439"
     CDS             complement(303..1439)
                     /locus_tag="OHO85_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCQ2673701.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1442..1804)
                     /locus_tag="OHO85_004440"
     CDS             complement(1442..1804)
                     /locus_tag="OHO85_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCQ2673702.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2341..4254
                     /gene="mtlA"
                     /locus_tag="OHO85_004441"
     CDS             2341..4254
                     /gene="mtlA"
                     /locus_tag="OHO85_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCQ2673703.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4484..5632
                     /gene="mtlD"
                     /locus_tag="OHO85_004442"
     CDS             4484..5632
                     /gene="mtlD"
                     /locus_tag="OHO85_004442"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCQ2673704.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGTLPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5632..6219
                     /gene="mtlR"
                     /locus_tag="OHO85_004443"
     CDS             5632..6219
                     /gene="mtlR"
                     /locus_tag="OHO85_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCQ2673705.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(6231..6440)
                     /locus_tag="OHO85_004444"
     CDS             complement(6231..6440)
                     /locus_tag="OHO85_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673706.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6725..7087
                     /locus_tag="OHO85_004445"
     CDS             6725..7087
                     /locus_tag="OHO85_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCQ2673707.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1867 a         1835 c         1847 g         1754 t
ORIGIN      
        1 taaatgtgac tgtcatcctt cagataataa agccatcatc gcaatagatg gaaaagcact
       61 cctgctcttt tttgattaga gccgtcgcag gaggaacaac tcatatcatc attgcgatct
      121 cgacgatgta tagtctcatc ctgcggcaga acaagacgga taaaaaatcc aatgagagca
      181 cactcatcca gaacttatta acataatgaa tattaaagaa aaatcatcgc agctaatgaa
      241 gtaatatgca aaaataatta gtccttacaa ggagcaaagt tccataatct cccccctccc
      301 cctcaatgat ccaaataaag ataatgcatc cagctggtca ttcttagcag cactttccgc
      361 atcaccgaaa catggctgaa atggtcggag taaaccgcca cctgggcgta gatgccgtcg
      421 ggtaaggcat cgatatcatc gttagggtcc agttcaatgg ttcccagcac accgtccgtg
      481 ccgggcacga ccgttaatga ttgcaatacc ccctgcgcct gataagaacc gcctggcacg
      541 acaggtaaaa tactggtcag tttgccgtgg aacacctgcc caggtagcgc gttaaacacc
      601 acttctgcat catcaccagg tttcagacgt aacagcgagt tttgccgaaa ttgggcgaca
      661 atttgccgtt tttgctcggg gatgaaaacc atcaccggac gcaacggcaa ggcagctgcg
      721 tatgtgcctg ggcggatcag tacctgagtg acgtagccat tgcttggtgc gcgaatgaca
      781 gtctgctcaa gattatattt tgcttcagta agttgcgctc ttaagctcac aatctgcgat
      841 tgctcgccgt taaccatact gtcgagctgg ctctggatct gcgcctgctc cgccaccgag
      901 cctttcacca gcgcatcctg cgcgaggaaa ttttgccgcg catcgtcgat gtcacgttcc
      961 gagaacggat tcaccgccgc ctggctgcct ttcagataac gttgataatt tttaaacaga
     1021 cggtcgcgct ccgctgaaac ctgggtggtg ttggcctgcg cttctgtgag ctgcgcgcgc
     1081 agagtcttta tattatgcgt cgccgtcatc aggtcagcct gaagcctgtc aactcgcgcc
     1141 tggtaacgaa ccgggtcgag cttaaaaagc acctcgccct tttgaataag ctgattattc
     1201 ttgtcagtga cttcagtaac aattcccgtc acctgtggcg tgatggggat cgctatcact
     1261 gccttttgcg cggtaaaagt gtaagggtgg ttgtagttca tcaacaaaat caaaccactc
     1321 accagaaaca cgccacccag cgccgccgtc gccagcgtcc actgattcac cggaatacga
     1381 aagattttaa agaccgccca cgccagcgcc acgtaagtta aaacaatcaa tagatccata
     1441 attagatctc cgggaacgtg gaatagtcag tctttttctc ggcggcgagt tgatgctcca
     1501 gccgggaaat tcggtcagaa agtgcggcga tttccgggtc agtcgctttc tcctgcgacg
     1561 caacatgtga ctgcataccc cagccacgct ccggttgata gagcgtcgcc cagatccaca
     1621 gaaacggcca gataacatgc agagtaaaca ggctcaccca gcccgccgta tgaatagcgt
     1681 cggcatgggg atggttgcgc tttttggcaa tcagataagg gatgtcatgt atcgcgatga
     1741 ttccataaaa aatcaccaga aagacgaaga tcagcactcc caacgcgaaa tagtttagaa
     1801 acatatctgc ctcggattca cgtttatcag tgttgttttt gggctggcag ccagaaggga
     1861 gtcaggctga tattttgaca ataatccggg ttcgcgattc tcgccataac accaaagaat
     1921 aatttttaga ggtgatgagt tgcttagtta cataacgatt gtatgacgaa ggcataacat
     1981 gctgtagatc acatcaggtg aacgccgtaa gaaaatatct tgtgattcag atcacaaaga
     2041 ttcaacaaac catcaaaaca aaaatgtgac actactcaca tttaaatgcc atttttagcg
     2101 aaaatcgccg ccttgttgct tttttacaca agcgttttgt gatgaacgtc acgtcaatta
     2161 cctctctacc ccctatattt atgtgattga tatcacacaa aaggccgtcg actggacagt
     2221 taaccgattc agtgccagat ttcgcagtat ctacaaggtc cggctacctc tgccgccaca
     2281 ttaacaaaaa acctcgggct tccagcctgc gcgacagcaa acataagaag gggtgttttt
     2341 atgtcatccg atattaagat caaagtgcaa agctttggtc gtttcctcag caacatggtg
     2401 atgccaaata tcggcgcgtt tatcgcgtgg ggtatcatca ccgcgttatt tattccaaca
     2461 gggtggttac cgaacgagac gctggcgaag ctggtcgggc cgatgatcac ttatctcctg
     2521 ccgctgctga tcggttatac cggtggtaag ctggtaggcg gcgaacgtgg cggcgtagtc
     2581 ggtgccatca ccaccatggg cgttatcgtc ggcgcagaca tgccgatgtt cctcggttct
     2641 atgattgcag gtccgctggg cggctggtgc attaagcact tcgaccgctg ggtagacggt
     2701 aagatcaaat ccggttttga gatgctggtg aataacttct ccgcaggcat catcgggatg
     2761 atcctcgcta ttctggcatt cctcggcatt ggcccgattg ttgaagccct gtccaaaatg
     2821 ctggctgcgg gcgttaactt catggttgtc catgacatgc tgccgctggc gtctatcttt
     2881 gttgaaccgg cgaaaatcct gttcctcaac aacgccatta accacggtat cttctcgccg
     2941 ctgggtattc agcagtccca tgaactgggt aaatcaatct tcttcctgat tgaagctaac
     3001 ccaggtccag gtatgggcgt gctgctggcg tacatgttct ttggtcgtgg tagcgctaaa
     3061 cagtctgcgg gcggtgcggc aatcatccac ttcctggggg gtatccacga aatctacttc
     3121 ccgtatgtgc tgatgaatcc gcgtctgatc ctcgcagtca tcctcggcgg tatgactggc
     3181 gtgttcacgc tgactatcct gggcggtggt ctggtttctc cggcatctcc gggttctatc
     3241 cttgctgtac tggcgatgac accaaaaggt gcttatttcg ctaacatcgc gggtgtgtgt
     3301 gcggcgatgg ctgtctcctt cgttgtctct gctattttgc tgaaaaccag caaagtgaaa
     3361 gaagaagatg atattgaagc agcaactcgt cgtatgcagg acatgaaagc tgagtctaaa
     3421 ggcacatctc cgctgtctgc gggcgatgtg actaacgacc tgagccacgt acgtaaaatc
     3481 atcgttgcct gtgacgccgg tatgggttca agtgcgatgg gcgcaggcgt gctgcgtaag
     3541 aaaattcagg atgcaggtct gtcacagatt tcggtcacta acagcgcgat caacaacctg
     3601 ccgccagatg tggacctcgt catcactcac cgtgacctga ccgaacgcgc tatgcgccag
     3661 gttccgcagg cgcagcatat ttcgctgacc aacttcctcg acagcggcct gtacaccagc
     3721 ctaaccgaac gtctcgttgc tgcccagcgc catactgaaa acgaagtgaa agtgaaagac
     3781 agtctgaaag acagctttga cgattccagt gctaacctgt tcaaactggg cgcggagaac
     3841 atcttcctcg gtcgcaaagc agcaaccaaa gaagaagcga ttcgttttgc tggcgagcag
     3901 ctggtgaaag gcggttacgt tgagccggaa tacgttcagg cgatgctgga tcgtgaaaaa
     3961 ctgaccccga cttatctggg tgagtctatc gcggtgccac acggtacggt tgaagcgaaa
     4021 gatcgcgtac tgaaaacggg cgtggtgttc tgccagtacc cggaaggcgt gcgcttcggt
     4081 gaagaagaag atgacattgc ccgtctggtg attggtattg cagcccgtaa caacgagcac
     4141 attcaggtta tcaccagcct gaccaatgca ctggatgatg agtccgtcat cgagcgtctg
     4201 gcacacacca ccagcgtgga tgaagtgctg gaactgctgg caggtcgtaa gtaatccaat
     4261 cccaccctct ccacatggag aaggtggggt taattgcctg atgcgctacg cttatcaggc
     4321 ctacaggatg catcacaatt tgttgaattt gcacgttctt gtaggccgga taaggcgctt
     4381 acgccgcatc cggcgcggcc cctctcctca cggagagggt ttgggtgagg gaaaagcctc
     4441 accccagccc tctcgggtaa aaacattgat gaaggttaat actatgaaag cattacattt
     4501 tggcgcaggt aatatcggtc gtggctttat cggtaaactg ctggcagacg cgggtatcca
     4561 actgacgttt gccgatgtca atcaggtggt acttgatgcc ctgaatgccc gtcatagcta
     4621 tcaggttcat gtggttggtg aaaccgagca ggtagatacc gtttccggcg tcaatgctgt
     4681 cagcagcatt ggtgatgatg tcgttgatct gattgctcag gtcgatttag tcactaccgc
     4741 cgttggcccg gttgtgctgg aacgtattgc tccggctatc gccaaagggc tggtgaaacg
     4801 caaagaacaa ggtaatgaat ccccgctgaa catcatcgcc tgtgaaaaca tggtacgcgg
     4861 taccacgcag ctgaaaggcc atgtgatgaa cgccctgccg gaagacgcca aagcgtgggt
     4921 agaagaacac gttggctttg tcgattccgc cgttgaccgc atcgtaccgc cttcggcttc
     4981 ggcaactaac gatccgctgg aagtgacggt agaaactttc agcgaatgga ttgtcgataa
     5041 aacgcagttc aaaggcacac tgccgaacat cccaggcatg gagttaaccg acaacctgat
     5101 ggcatttgtc gaacgtaaac tcttcaccct gaacacgggt catgctataa ccgcgtacct
     5161 cggaaaactg gctggtcatc agaccattcg tgacgctatt ctcgacgaga aaatccgcgc
     5221 ggtggtaaaa ggtgcgatgg aagaaagtgg tgcggtactg atcaagcgct acggctttga
     5281 cgcagacaaa catgcggcgt acatccagaa aatcctcggt cgttttgaga acccgtatct
     5341 gaaagatgat gtagagcgcg taggccgtca gccgctgcgt aaactgagtg ctggcgaccg
     5401 tctgatcaag ccactgctcg gtacgctgga atacagcctt ccgcacaaga atctgattca
     5461 ggggattgct ggtgcaatgc acttccgcag tgaagatgat ccgcaggctc aggaactggc
     5521 agcactgatc gctgacaaag gtccgcaggc ggcgctggca cagatttccg gtcttgatgc
     5581 caacagcgag gttgtatccg aggcggtaac cgcttataaa gcaatgcaat aatggtggac
     5641 caggcgcagg acaccctgcg cccgaataac agattgtcag atatgcaggc aacaatggaa
     5701 caaacccagg cctttgaaaa ccgtgtgctt gagcgtctga atgctggcaa aaccgtgcga
     5761 agctttctga tcaccgccgt cgagcttttg accgaggcgg taaatcttct ggtgcttcag
     5821 gtattccgca aagacgatta cgcggtgaag tatgctgtag aaccgttact cgacggcgac
     5881 ggtccgctgg gcgatctttc tgtgcgttta aaactcattt acgggttggg cgtcattaac
     5941 cgccaggaat acgaagatgc ggagctgctg atggcattgc gtgaagaact aaatcacgac
     6001 ggcaacgagt acgcctttac cgacgacgag atccttggac cctttggtga actgcactgc
     6061 gtggcggcgt taccaccgcc gccacagttt gaaccagcag actccagttt gtatgcaatg
     6121 caaattcagc gctatcaaca ggctgtgcga tcaacaatgg tcctttcact gactgagctg
     6181 atttccagaa tcagcttaaa aaaagccttt caaaagtaag caacgtctgc ttactgcccc
     6241 tctacctgct tcggccgata aagccgacga taatactcca gacgttgtag atataacggc
     6301 acgctttcat cgggtattcc ggacggaatc gcgttacggg gaggaagttt tttcagatac
     6361 tcccggaacg cctggcttga tgccatgaaa tctacggctt tatcgataag aagcggaacg
     6421 ttttcaccta atttgcccat gatgatatct ccgtattacc ccgcgccggg aatgcgcggc
     6481 cgccaatttt agtttaggtt cccggtaaag atgacattaa ggaaaacgtg ctgaatcctc
     6541 aataatcatc cgctgctgtt tattatcttt tttctataga tttcttaatt aatcaacgaa
     6601 ttgcgtagtt tttgagcatg ttaatgatta cgttgcagaa ctattacaat gtcatcagtt
     6661 gtgaacaaag cacctggtcg cgcatactag gggctataaa tttatcttta tcagaagcca
     6721 tcacatgaaa gaagtcgaaa aaaacgaaat caaacgtctc agcgatcgcc tggacgccat
     6781 ccgccaccag caggccgatc tatcgctggt tgaagccgca gacaaatatg ccgagctgga
     6841 aaaagagaaa gccacgctgg aagcagaaat tgctcgcctg cgtgaggttc atagccaaaa
     6901 actgagtaaa gaagcacaga agctgatgaa gatgccgttt cagcgcgcaa ttaccaaaaa
     6961 agagcaggct gatatgggca agctgaagaa aagtgttcgc ggactggtgg ttgtgcaccc
     7021 aatgaccgcg ctgggccgtg aaatgggcct gcaggagatg actggattct ctaagactgc
     7081 gttttaagcg cctcagacga ctggttcgat catatcaacc cgcatctggc ttgccttagc
     7141 tggtcaatgc ggggactctc ctgtctactc gcctaattac caaacaattc actcccttta
     7201 cgtcgattta ccgctggttg atattctgaa aatgcatttt taccgaaata gccacctata
     7261 aacaataaaa atatatagaa gatatcattt attaattaac aag
//
LOCUS       DAJOWB010000185         7208 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJOWB010000185 DAJOWB010000000
VERSION     DAJOWB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.185"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            134..1528
                     /locus_tag="OHO85_004446"
     CDS             134..1528
                     /locus_tag="OHO85_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCQ2673708.1"
                     /translation="MYSLKQLVISGRKRRWILMDLETSLPLLYPLRYHIDHLAFRSLS
                     TQSASLQSVKFFYEFWRQKYGVSFCYSFYSSDHNPDIAVGEMPAFWMYLENGHNVQSN
                     VLSLTRVTKANSLTHTVRVRAVIHFISFLINTYISPAYRDDSPKALSLLASRLHTRLQ
                     LCRENYRTLTSNKFSQHSHSSQGFQSLSGAMVLSLYGIITPSSAQKHNPLNPFPSGHL
                     QFRNFLIIRLLLNYGLRTGELLLLECSSIKPNLKGDKFSLIVTTVDEAYEPRKNAPSL
                     KNAWANRVLELDKQDYIFLSIYIAKLRPKTDKHDFIFTSSQQSAKPLSYTSVHSIFSK
                     IDQVFTNQYPEYKSPLYSDSLQRLTPHTTRHTWAFLTLQKIWHLKYLKSQQNKTHFIA
                     EVPSLSGIMEEAKDELRLMGGWSPTSQMPDLYAKRFLSEQANAANVQRIIQDNAALHN
                     TLDTIMDRYNDDII"
     gene            1515..3080
                     /locus_tag="OHO85_004447"
     CDS             1515..3080
                     /locus_tag="OHO85_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCQ2673709.1"
                     /translation="MTSFDNALKSDEGNSVYPPDLAKAIFELDIPDRIYLKTAMEFDD
                     KYIINKGNKWTFDYSGSRRYLVFNQDYISNKLNKYICFKYASTRFPTKLPALHLDWRN
                     IISYCREHGGFSLSTLRLFIEKEDIDNATFYSVLFGLKILCAETFPGFNLEDYDDLEF
                     VPRPYLEHWGVYQEIDNILDPLEKNMICNGLFEMASLLRDGKSFSHSEVRNAAILGLA
                     YVTGARPVQLARLAVKDVRIDTRNQESGLIRYSVFLPYAKQRRVTTERLFLAIPPEIG
                     ELIMNYTVRYKKNPEDKLFDFSVSAPHYVSQAINQAILNFCPPEYQAAVACGEAALPT
                     ITPTDLRHNVGHSLAMQGASAEEIAHVLGHTSLAVAKYYIMATPALALIRAKALGSNP
                     VWQNMVAMMLTGELVDSAHWKGHPVVGLVGDELHDKIGGCSRNSSTCPFSEVRSCYGC
                     LYYRPFTDGEHQALLECVKKEVDELIAISDGVGNSRNPLILIHETTQFEIESVIARCR
                     FHQEQVKSNEKSL"
     gene            3064..5430
                     /locus_tag="OHO85_004448"
     CDS             3064..5430
                     /locus_tag="OHO85_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000716055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673710.1"
                     /translation="MKNHFEHFQAFIEEQKKWFEQHLSADFAQTWNDHIWVSGNQGSG
                     WLRGNGRNTLRFDEMNRFKGIVEHLPIDKEYSQFMKAMLVVVYRQGNRSMSPAVAKAT
                     LMILKRWYHAMLYETGQTHPSYLSTVVIQRAMDLLSSVSSPSDPNTANYKGRCVSLQK
                     LVNHFTFTLVKLQYNSDVKYTNKTNLTRKARETMALKQQAKLSDTTTDGEDALITIRG
                     FLNIVALIQRVESDAEKIALNCLLLLVITGFRSIEAFNLRQDALFKRQIDDPAICKRF
                     QDKGLPDYFLGIRYVGVKGAGERTHWVEPLAVPLVESIFSTVKMLTAPIRSHLTYLRA
                     KSFADYLPQAISNMPGERVELDDVVKYITQTTSSFRGRAGQRDKTSKALSKRGVLPVQ
                     EIPGPKNSKSIYYSKTALNHYIKTEFGLANANEPCTHAWMENGKRYQVNYEDLLFLHE
                     KGSLALKRTLALLATPIPFTNTLMNKFLGNVEPDGSVFSKYQLLEEDGTSTRMRTHIP
                     RHNINTFLAIADISDHLQAMLMGRVDITQNQHYQHLALAERRKAASLVPLQPISTALT
                     VSPSSSSIATPLDIVKRTGQLAVTEHMTLDNTIKANLHTFDDRDDVARFVEASFANGL
                     FEDVAAAFEEIRDAEGPEQASDMVARHAVLYPLKFGSCMREVNLWGCPYRLKCQSAAF
                     CEHFTLTGRIDELPNLIAKKQALQQAHSKLTQLTQHQPDYQTRLADIERRLQQLEAIQ
                     AQWHRRAKTQQLVAAESVLSGGVITEGKVRTLAQLFALEYQQLMQEND"
     gene            5430..5864
                     /locus_tag="OHO85_004449"
     CDS             5430..5864
                     /locus_tag="OHO85_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673711.1"
                     /translation="MRGKELNTLIEHELQLMLVEGFDKSPISAKALHIRLKAKGIVNG
                     GLSTLSSLERKRLIAAYVDQQLGPLNLRPKEKQQYVNRKTRQALLARNQQLQAEVSEL
                     RDQLAQNTLSLIEIVKAVKINSVIPVESLLANHMIRELHKSS"
     gene            6231..7208
                     /locus_tag="OHO85_004450"
                     /pseudo
     CDS             6231..7208
                     /locus_tag="OHO85_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611474.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISSen4 family transposase"
BASE COUNT         1971 a         1647 c         1648 g         1942 t
ORIGIN      
        1 ggtaaataag ttcatatgac actcccacta actgataaat caatacgtta ggatttctga
       61 tctccctctc gtttcttgca ttaggtacag agtacattct cttcatatcc atgataattt
      121 tgaggtaaat attgtgtatt cactaaagca gttggttatc tcaggccgta aaaggcgatg
      181 gatcctgatg gacttggaaa cctctctgcc gttgctgtat ccccttaggt accatattga
      241 ccatttggct ttccgctcat tgtccacaca gtcagcttca cttcaatctg tgaagttttt
      301 ttacgaattc tggaggcaaa agtatggtgt ctcgttttgc tacagcttct attcatctga
      361 ccacaatcca gacattgctg tcggtgaaat gcccgcattt tggatgtatc tagaaaatgg
      421 ccacaacgtc cagtcaaatg tgttgtcttt aacgagagtc acaaaagcca atagcttaac
      481 tcatacagtt agagttcgtg ctgtgatcca tttcatttct tttttgatta acacctacat
      541 atcacctgca taccgggatg actctcccaa agcattatca cttttggctt cgcgtttaca
      601 cacccgttta cagctttgcc gggagaatta tcgaacgctt acatcaaaca aattttcgca
      661 acatagtcac agcagccaag gatttcagag tttgtctggg gcaatggtgc tcagcctgta
      721 tggaataata accccctctt ctgcccaaaa gcataacccg ctaaatcctt tcccttccgg
      781 gcatcttcag ttccgcaatt ttttgattat tcgtctgttg ttaaattatg gtttacgaac
      841 aggtgagcta ttgttgttag agtgcagctc aattaaacct aatttaaaag gtgataagtt
      901 tagtttgatt gtgacaactg tcgacgaggc atacgaacca agaaagaatg cgccatctct
      961 caaaaatgca tgggctaatc gtgtgttgga gctcgataag caggattata tttttttaag
     1021 catttatata gcaaagcttc gaccaaaaac tgataaacac gatttcatct ttacatcgag
     1081 tcaacagtca gcgaagcctt tgtcctacac atcagttcat tctatttttt cgaaaataga
     1141 tcaggtcttc actaaccaat atccagagta caaatcaccg ttgtactctg actcgttgca
     1201 acgattgact ccgcatacaa cccgacatac ttgggccttt ttgacattgc aaaaaatatg
     1261 gcatttaaaa tatttgaaaa gccagcaaaa taaaacacac tttattgctg aagttccttc
     1321 cctctcaggg ataatggaag aagccaaaga tgaactacga ctgatggggg gatggagtcc
     1381 gacaagtcaa atgcctgatc tatatgcaaa acgtttcctg tctgagcagg ctaatgctgc
     1441 caatgtccaa cgaattattc aagacaatgc agcgctacat aatacgttag atactataat
     1501 ggatagatat aacgatgaca tcatttgata atgcattaaa atctgacgaa gggaactcag
     1561 tttaccctcc tgatttagct aaggctatct ttgaactcga tattcctgat cgaatttatc
     1621 taaaaacggc aatggagttt gatgataaat atattattaa caaaggaaat aaatggacct
     1681 tcgattattc aggcagtcgg cgatatcttg ttttcaatca agattatatc tcaaataaat
     1741 taaacaagta tatctgtttc aaatatgcat cgacgagatt tccaaccaaa cttccagcgt
     1801 tgcaccttga ttggaggaat atcatttcat attgtcgaga acatggtggg ttttcgctta
     1861 gtacgctccg attatttatc gaaaaagagg atattgataa tgctactttt tattccgtgt
     1921 tattcggatt aaaaatactt tgtgctgaaa cattcccagg tttcaatctt gaagattacg
     1981 atgatcttga gttcgtccct cgaccgtatc tcgaacattg gggggtttac caagaaatcg
     2041 ataatattct tgaccctctt gaaaaaaaca tgatttgcaa tggactgttt gaaatggctt
     2101 cgttgttacg cgatggcaaa agtttcagtc acagcgaagt gcgcaatgca gctatccttg
     2161 ggttggctta tgtaacgggt gcacgcccag tacaactcgc taggctggcg gtcaaagatg
     2221 ttcgtatcga tacacgaaat caggaatctg gtctgattcg atacagtgtt tttttaccat
     2281 atgctaaaca aagacgagtg acgacagagc ggttatttct ggcgatcccc ccagaaattg
     2341 gggagttaat tatgaactat accgtgagat ataagaaaaa ccccgaagat aaactattcg
     2401 atttcagcgt ttcagctccg cattatgtgt ctcaggcaat taatcaggca atcctaaatt
     2461 tctgtcctcc tgaatatcaa gccgccgtag catgtggcga agctgcattg cccacaatta
     2521 cacccactga tttacgccac aatgttgggc actctttggc gatgcaaggt gctagcgctg
     2581 aagagatagc acatgtactg gggcacacct ctcttgccgt agctaaatac tacattatgg
     2641 caactcccgc gttggccttg atccgtgcta aagcgcttgg gtcgaatccc gtttggcaaa
     2701 atatggtggc tatgatgctc actggggaac tggtcgactc agcacattgg aaaggccatc
     2761 cagtggtggg actcgttggt gacgagttgc atgataaaat tggtggttgc tccaggaaca
     2821 gtagtacatg ccctttcagc gaagtgcgtt cttgttacgg ctgtctgtat tatcgccctt
     2881 ttacggatgg tgaacatcag gctctacttg aatgcgtcaa aaaagaagtc gatgaactaa
     2941 tcgctatttc agacggtgta ggtaattccc gaaatccgct cattttaatc catgaaacca
     3001 cacagtttga aattgagtct gttatcgccc gctgccgttt tcatcaggaa caggtgaagt
     3061 ccaatgaaaa atcactttga gcattttcag gcttttattg aagagcaaaa aaaatggttc
     3121 gaacagcacc tgtctgctga ttttgcccaa acatggaatg atcatatctg ggtaagtggt
     3181 aaccagggaa gtggatggtt gcgagggaac ggcagaaata cattacgttt tgatgagatg
     3241 aacagattca aaggtattgt agaacatctg cccatcgaca aagaatatag tcagttcatg
     3301 aaagctatgc tggtagtggt ttaccgacag ggaaatagga gtatgtcgcc agcggtggca
     3361 aaggcaactt tgatgattct gaaacgatgg taccatgcga tgctttatga gacaggacag
     3421 acccatccat cttacctgag cacagttgtc attcaacgag cgatggatct tctcagtagc
     3481 gtttcatctc ctagtgaccc caatacagct aactataaag ggcggtgcgt tagccttcaa
     3541 aaattggtca atcacttcac atttacgttg gttaaactgc aatacaactc tgatgtgaag
     3601 tataccaaca aaaccaactt gacccgcaaa gcccgggaaa ccatggcgct gaaacagcag
     3661 gccaagctgt ctgataccac taccgatggt gaagatgcgt tgattaccat tcgcggattt
     3721 ctgaacatag ttgcattaat ccagcgggtg gaaagtgatg ctgagaaaat cgcgttaaat
     3781 tgcctgttgt tattggttat taccggcttt cgctccattg aagcgtttaa tctcagacag
     3841 gatgccctgt ttaaacgcca gattgatgat ccggcaatct gcaaacgatt tcaggacaag
     3901 ggattacccg attattttct tggtatccgt tatgttggtg tcaaaggtgc tggggagcga
     3961 acgcattggg tagaaccgct ggcagtaccg ctggtcgaga gtatattcag taccgttaaa
     4021 atgttgaccg caccaatacg cagccatctt acctatctac gggcaaaatc ttttgctgat
     4081 tacctgccac aggccatcag caacatgccc ggtgaacgag tcgaactgga cgatgtggtg
     4141 aagtacatca cccaaactac ctcaagtttt cggggccggg caggacaacg ggacaaaaca
     4201 tccaaagccc ttagcaaaag aggtgtgctg cccgttcaag aaataccagg cccgaaaaac
     4261 agcaaatcta tctattattc caagactgct ctcaatcatt acattaagac cgaatttggc
     4321 ctggcgaatg ccaacgaacc ctgtacacac gcctggatgg aaaatggcaa acgctaccaa
     4381 gttaactacg aggatctgct atttctacac gagaagggtt cgctggcatt aaagcgcaca
     4441 ctggctttgc tggctacacc gatacccttc actaacacac ttatgaataa attcttaggc
     4501 aacgttgagc ctgatggatc agtattcagc aaatatcaat tactggagga ggatggcacc
     4561 tcgacgcgga tgcgtaccca cattccccgg cacaacatca atacctttct tgctattgcg
     4621 gatatctctg accacttaca ggcaatgttg atgggtcgag tggacatcac gcagaatcag
     4681 cattatcaac atctggcgct tgcagaacgg cgaaaagccg cctcgttggt gcccttgcag
     4741 ccgatttcga cagcactaac tgtctcgccc tcttcgtcct ccattgctac gccactggat
     4801 atcgtcaaac ggaccggcca actggcggtc acggaacaca tgactttgga caataccatc
     4861 aaagccaacc tgcatacttt tgacgatcgg gatgatgtag ccaggtttgt cgaggcgtca
     4921 tttgctaacg gcctctttga agatgttgct gccgcctttg aagaaatccg cgacgcagaa
     4981 ggaccagagc aagcctctga catggtggca agacatgccg tgctgtatcc gctgaaattt
     5041 ggctcctgta tgcgggaagt taacctctgg ggatgtcctt atcgactgaa atgccagagt
     5101 gctgcctttt gtgaacattt cacgctgaca ggccggatag atgagctacc aaatctgatt
     5161 gccaaaaaac aggctttaca gcaagcacat tcgaagttga cccaacttac tcaacatcag
     5221 cctgattacc agaccagact ggccgacatc gaacgacggt tacagcaact cgaggcaata
     5281 caagcacaat ggcatcgtcg tgccaagact caacaactgg ttgctgctga gagtgtgttg
     5341 tctggtggag taatcaccga gggcaaagtc cgtacactgg cccagctttt tgcacttgaa
     5401 taccaacaat tgatgcagga gaatgattga tgcgcggcaa ggaactgaac acactgattg
     5461 agcacgaatt acaactgatg ctggttgaag gctttgacaa gtcccctatt tccgccaagg
     5521 ccctgcatat taggctcaag gcaaaaggta tcgttaacgg tggtttaagt acgctgagca
     5581 gcctagagcg taaacgccta attgctgcct atgttgacca gcaacttggc ccattgaatc
     5641 tacgcccaaa agaaaaacag caatacgtca accgcaaaac ccgtcaggct ttgctggcca
     5701 gaaaccagca gttgcaggct gaagtaagtg aattgcgaga ccaattagcg caaaataccc
     5761 tttcgttgat agaaattgtc aaagcggtaa aaatcaattc ggttataccg gtggaaagcc
     5821 tgttggccaa ccatatgatt cgggaactac ataagtcgtc ataattctga catggaagat
     5881 ttagccgaca gcttcccggt tgtcggcaga atgctggttg tattgataaa catatattta
     5941 tggctccctc ttttgagttt gttatctgag agggcgtctc ccactgtaat gtcctgcttg
     6001 gttcattgca tcagtgtaat gctacacttt cgttaccttc aaaaagttca gtcgctttat
     6061 atgtaatatc attttttgtc agcatgcttt tcgcctggat gccttttgag tgcttcgata
     6121 aaattacttc gctcttcctc tgtcatccgg gatatgagtt gacctgctcc ccgttgatta
     6181 gtacaccccg atgttagtaa tgtcttcata agccacatga ggacatcccc atgaagaagc
     6241 gtttttccga cgaacagatc atcagtattc tccgcgaagc cgaagctggg gtacccgccc
     6301 gtgaactctg ccgcaagcat gccatttccg atgccacgtt ttacatctgg cgtaagaagt
     6361 atggcggtat ggaggtgcct gaagttaagc gcctgaagtc gcttgaggaa gagaacgcca
     6421 gactcaagaa gctgcttgcc gaagccatgc tggataaaga ggcgcttcag gtggctcttg
     6481 ggcgaaagta ctgacgacag accagaagcg ggaagccgtg atgttgatgt gtgatgcgac
     6541 cggtctgtcg caacgtcgtg cctgcaggct tacaggttta tccctgtcga cctgccgcta
     6601 tgaggctcac cgtccggctg ctgatgcgca tttatcaggg cgcatcactg agctggcact
     6661 ggagcgcagg cgttttggct accgtcgtat ttggcagttg ctgcgccgtg aagggcttca
     6721 tgttaatcat aagcgcgtgt accggcttta tcacctcagt ggcctgggcg taaaacgcag
     6781 aagacgtcgt aaagggctgg caacagaacg tctgccgctg ctccgtccgg cggcgcccaa
     6841 tctgacctgg tcgatggatt tcgtcatgga cgcactttcc accggtcgca ggatcaagtg
     6901 ccttacctgc gtcgatgatt tcacaaagga atgcctgacg gtcactgttg cctttgggat
     6961 ttcaggcgtt caggtcacgc gtattctgga cagcattgca ctgtttcgag gctatccggc
     7021 gacgataaga actgaccagg ggccggagtt cacttgccgt gcactggatc aatgggcctt
     7081 tgagcatggt gttgagttgc gcttaatcca gccgggcaag ccaacgcaga acggatttat
     7141 tgagagcttt aacggacgat ttcgcgatga atgtttgaat gagcactggt tcagcgatat
     7201 cgttcatg
//
LOCUS       DAJOWB010000186         7156 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJOWB010000186 DAJOWB010000000
VERSION     DAJOWB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.186"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(126..761)
                     /gene="msrQ"
                     /locus_tag="OHO85_004451"
     CDS             complement(126..761)
                     /gene="msrQ"
                     /locus_tag="OHO85_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCQ2673712.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(762..1766)
                     /gene="msrP"
                     /locus_tag="OHO85_004452"
     CDS             complement(762..1766)
                     /gene="msrP"
                     /locus_tag="OHO85_004452"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCQ2673713.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISATALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPYVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(1875..2288)
                     /gene="hiuH"
                     /locus_tag="OHO85_004453"
     CDS             complement(1875..2288)
                     /gene="hiuH"
                     /locus_tag="OHO85_004453"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCQ2673714.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVNAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2421..3092
                     /gene="hprR"
                     /locus_tag="OHO85_004454"
     CDS             2421..3092
                     /gene="hprR"
                     /locus_tag="OHO85_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCQ2673715.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3092..4450
                     /gene="hprS"
                     /locus_tag="OHO85_004455"
     CDS             3092..4450
                     /gene="hprS"
                     /locus_tag="OHO85_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCQ2673716.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDGMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     NVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNSYLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(4548..5399)
                     /gene="hchA"
                     /locus_tag="OHO85_004456"
     CDS             complement(4548..5399)
                     /gene="hchA"
                     /locus_tag="OHO85_004456"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCQ2673717.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(5911..6222)
                     /locus_tag="OHO85_004457"
                     /pseudo
     CDS             complement(5911..6222)
                     /locus_tag="OHO85_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6236..>7156)
                     /locus_tag="OHO85_004458"
     CDS             complement(6236..>7156)
                     /locus_tag="OHO85_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HCQ2673718.1"
                     /translation="WTGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWM
                     DGRRSRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMR
                     QNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDR
                     EALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFAR
                     MLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWH
                     PHSALGYRSPREYLRQRACNGLSDNRCLEI"
BASE COUNT         1886 a         1615 c         1715 g         1940 t
ORIGIN      
        1 aaattatttt ggtgatttga ggttgatgac aaacgaaaca taacctgatg cgatctgtgt
       61 atgccggata gggcgctcgc gccacatccg gcagggataa cgcgtacttt gtcggcaata
      121 tgaagttata cagataattt gttgtgaacc tgcttgcgta accggttaaa aagggaacgc
      181 aacttcttat accgtaaggc taaaagcagt acagccagcc cagcgtagat gagcggctgc
      241 ggtgaaataa tcttcactga ccacagataa tgtattgggg ccagaatcgc gacaagatag
      301 acgaaattat gcaactgttg ccagtgcttg ccgagttttc gctgcatcgc ctgggttgaa
      361 gtgaacgcta aagcaagcaa aattacccag ctgataatac ctaacgttaa ataaggccgg
      421 gtaattaact ccttacctaa taacgccaga ttattcacgc ccagctccag caatgcgtaa
      481 cttgttaaat gcagtgtcgc ccaagcaaag caccataatc ctaacaggcg gcgagtgcgt
      541 atcaataacg gctgttttgc gtagcgtgcc agaggtgtga ttaacaaggt cgccagcaaa
      601 aatttcagtg cagtgcgacc agtaaaatgc tgaatatctt tcaccggatc ggcacccagt
      661 ccaccgtgat tgatcgccca gaccagccag agaaacggca acaatccggc aagatgcagg
      721 caaactttca gccatgtcac ctgttttgct gtcagacgca tttagaaatt ctcccgcaaa
      781 tccaggccac gatacagcga tgccacctgg tcggcgtaac cattaaacag tagcgttggc
      841 tggcgctgta catcgaggat gccgcctgaa ccaataaatc gttcggtagc ctgtgaccag
      901 cgcgggtgat caacatacgg attaacgttg gcgtaaaaac cgtattcgtc aggcgctgcc
      961 agattccagg tggttggcgg acgctcgcgg gtcagcttaa tactgactat cgatttaatc
     1021 cctttaaagc catatttcca cggcacaatc agtcgcaccg gcgcgccatt ttgtggcggt
     1081 aacgccttgc cataaacacc tacggtcatc agtgtgagcg gatgcattgc ttcgtcgaga
     1141 cgcaatcctt cgacataagg atatttcagc ccaccaccga taaagcggtc ctgctggcct
     1201 ggcatctgtt ccggtgcata aattgtttcg aaagcgacat acttcgcatt gctggtaggt
     1261 tcggcaagcg ccagcaattt gtgcagcgga aaaccaatcc acggcaccac catcgaccac
     1321 gcttccacgc agcgcatacg ataaatacgc tcttccagcg ggaagcgacg ggttaagtca
     1381 tcatgatcga gggtcaatgg ttttgccact tcgccgctga ttttcagtgt ccatggatcg
     1441 gttttcaggc taccagcatt agcggcggga tcggctttat ccagcccgaa ttcatagaag
     1501 ttgttataac cggaaacttt atctgctggc gtcagtggca ggttattttg ccaggcggca
     1561 ggcttgctga actccagcgc ttttccggcg ggggcgggcg ggcgatcgtt ccctttaaac
     1621 cagctaagca gatcggcatg cgcagcgtga ggcaaagaaa gtgcagttgc actgatgccc
     1681 agtgctttta acacctgccg acgcttcata aagaataccg actcggccgt aacatctgat
     1741 tcttttaaaa attgattctt tttcataagc catcacactt ttttgtcgtt agccagcaag
     1801 agccgtttac tgttaatctg tcagggagtg tcctccctaa cagtagcaga gatctgcttt
     1861 tacatattta tgcattaact gccacgatag gttgaatacc catattggct aagtaataaa
     1921 ggcacatgat aatgctcgtt cactttatta atatgaaatt caacggggat ctcagggaag
     1981 aaactttcaa gattttgttt cttgaaatag tccccggttt taaatacgac acggtaatcg
     2041 cccgtagttg cagtttgctc gggccacagt gccttaattc gtccatcctt atctgttttg
     2101 gcggtattaa gttgtaacca gccgttatcc gccttctttt caagagtgac tgtcacgtca
     2161 gcagcgggtt ttcctgtttg ctggttcaaa atgtgcacgc taagaatgtt ttgttgtgcg
     2221 gcattaacca aagaaggtaa tgaaaatgct gccgttgcta ccgagagtac taaataacgc
     2281 tttaacatgt ttatatcctt gtcatgtgaa tgagttttga ggatagcgaa aaagcgtcca
     2341 gggtaacctt acagcagcat tacaattttg taatgaacca ggctgtttct ataacatatg
     2401 atttatggca tattattttc atgaagattc tacttattga agataatcaa aggacccagg
     2461 aatgggtaac gcaggggctt tccgaagcgg gttatgtcat cgatgccgtt tctgatggca
     2521 gagatgggct ttatcttgcg ctgaaggatg attatgcatt gatcattctg gatattatgc
     2581 ttccgggtat ggatggctgg cagatcttac aaacgttaag aacagcaaag caaacccctg
     2641 ttatttgcct tactgcaagg gattctgtcg atgacagagt cagagggctg gacagtgggg
     2701 caaatgatta tctggtaaaa cctttttcat tttctgagtt gctggcaagg gttcgggcac
     2761 aattaaggca acatcacgct ttgaattcaa cattagaaat cagcggctta agaatggact
     2821 ctgttagtca aagtgtgagc agggacaata tcagtattac actgacgcgc aaggagtttc
     2881 agttactttg gctactggcc tccagagctg gcgaaattat acccagaacg gttattgcga
     2941 gtgaaatttg gggaatcaac tttgatagtg ataccaatac ggtggacgtc gccattcgca
     3001 ggctccgcgc aaaagttgat gatccttttc ctgaaaagct aatcgccaca atccggggga
     3061 tgggctattc attcgtagcg gtaaaaaaat aatgaaaaga ttatctataa ccgtccgttt
     3121 aaccttgctt tttatattgc tgctgtctgt tgctggcgct ggaattgtct ggactctcta
     3181 taatggcctg gcaagtgagt tgaaatggcg cgatgataca acactcatta accggacagc
     3241 acagatcaag cagttgttaa ttgatggggt aaatccagat acgttacctg tgtactttaa
     3301 ccggatgatg gatgttagtc aggatatctt gatcattcat ggtgatagca tcaataaaat
     3361 tgttaaccgg acaaatgtca gtgatggcat gttaaataac atacctgcta gtgagacaat
     3421 cagcgcagct ggcatttaca gaagcattat taatgacaca gagatagatg ctttacgaat
     3481 taatattgat gaagtttcgc catcattaac ggttactgtg gctaaattgg cttcagccag
     3541 acataacatg cttgaacagt ataaaatcaa tagcattata atttgcattg tcgccattat
     3601 actgtgctca gtattaagtc cgctgttaat ccgaacgggg ttacgagaga tcaaaaagtt
     3661 gagtggtgta acggaagcgc tgaattataa cgatagccgg gagcctgttg aggttagtgc
     3721 attaccgaga gaactaaaac ctcttgggca ggcgttgaat aaaatgcatc atgctttagt
     3781 caaagatttt gagcgtctaa gtcagtttgc tgacgatctc gctcatgaac ttagaacgcc
     3841 aattaatgca ttactgggtc agaatcaggt tacgctcagt caaaccagaa gtatcgctga
     3901 atatcaaaaa acaattgccg gaaacattga agagctggaa aatatttcgc ggttaacaga
     3961 gaacatactg tttcttgcca gggcagataa aaacaatgtt ttggtgaaac tggactcgct
     4021 ttctctcaat aaggaagtcg aaaatttgtt ggactatctt gaatatcttt cagacgagaa
     4081 agagatttgc tttaatgtcg agtgcaatca gcaaatcttt gcggataaaa ttttactgca
     4141 acgaatgtta tcgaatctta ttgttaacgc tattcgatat tcgccagaaa aatcgcgtat
     4201 tcatataacc agttttcttg ataccaacag ctatcttaat attgatatcg ccagccctgg
     4261 aacgaaaatt aatgagcctg aaaaactctt ccgtagattt tggcggggag ataattcgcg
     4321 tcattccgta ggtcagggac ttggtctttc tttagtcaaa gcgattgccg aattacatgg
     4381 gggaagtgct acgtatcact atctcaataa gcataatgtg ttccggatta cgttaccgca
     4441 aagaaattaa catagggctt cagtacgccc gtgaaaaaac gcagccacgt cgtgagtact
     4501 aacgcggctg cgattgatta tgcgcttaca ttcaaacgta acagggatta acccgcgtaa
     4561 gctgccagca tttcctgcgc cgccagttta cccaacgcat tcgctgcaaa aggactgtcg
     4621 ccggtgagaa gtttacggtc cttatgtact cgcccggtga tgtcgtcatt aatgatgttc
     4681 atgcccattt tcttcagttc ttcgccgaag taccaggtga gatgacccgg catatagcca
     4741 atctctggcg tttgtttgtc tgcggcgtct gggaatgcgc aaatggaata accattcagt
     4801 gggttatcgc cgtggcgaag cgccagaaaa gccgccgggc cgtggcaaag ggagataaca
     4861 aaacggtcat ttttgattgc ccactgtaaa gcggcagcta cgtcctggct ttcaggtaaa
     4921 ccaataagtg cgccatgacc accaggaaca aagattgctg catattcgct atcagcgttg
     4981 aggctggcga caacatccgc gagtttcttc ggattgcgga acaacgattt atgctgctca
     5041 aagaatggca ttactttttc atctttgtgc ggcatagccc agtattcaaa cttggtcatc
     5101 agaccggaaa tggtcgccac ttcgaattcg aaacctgcag catggagatg atacaacggc
     5161 agcaacgttt caatcggatg gttaccggtc gagaacagtt ttccgttatc ggtcggcaaa
     5221 taacgttcgt ccgcggcgat caccagaatt ttatgtttac cgcgataagg ttttggatag
     5281 tccacgccat caagatcaga gacaggactg gtatattggc taagcgaata ttctgaaggg
     5341 aagaatgcat tatcttcagc aatatcgacc tgcggatttt tacttgtttg aacagtcata
     5401 gtgtattcct tattgttgct tagttagggt agtcactata tttgcgactg agctggtcgc
     5461 tatttttaac caggctggaa aattgagtca atatatcgaa agcggtagtc ggcgggtgta
     5521 agtatgtaat gatgatgctg cactggtgtt gaatattatt cacgccaaat tttccttatt
     5581 gagcaacgtc gtcgcaagtg gcctcaatga ttttagacca ttatcagagg tacagggcgg
     5641 cggggagaga tttagtgctg tggaatattg tggtgctgat gaatatttat atcccggatt
     5701 ggtagagaaa aagctttttc catgcacttt ttttgcaaat atttctatat gggaagtgtg
     5761 tttgttgcgg attaattcat aaagaaatgc atttggggtg ttggcttgaa gagtcgctgg
     5821 ttgatgttat tgtgccagga ggggcagggt aatgtgccgt aacgatatga ggtgtactgg
     5881 caatagcgga cactaccatt tgttcttttt ttaagcagcc atctgatgat atttttccct
     5941 gaaggctgcc ggggagatat tccccagacg agagtgacga cgctgacgat tgtagaaaat
     6001 ctcaatgtat tcccgtatta ctgagatggc ttcatcccgg ttattaaaac gatagtggct
     6061 caggctctca tttttcagcg ttccccagaa gctttccatc ggagcgttgt cgtaacagtt
     6121 acctttacgc gacattgatg ttttcagacc aaactgctcc tgtatgaccc ggtaatcgta
     6181 tgcgcagtac tgtgaacctc gatcagagtg gtggattagc ccggtggatt tgcccctata
     6241 tttccagaca tctgttatca cttaacccat tacaagcccg ctgccgcaga tattcccgtg
     6301 gcgagcgata acccagcgca ctatgcggat gccattcgtt ataatgctcg aacgcctctg
     6361 caaggttctt tgctgccgtt aacccgtctg gtttgggcat gatactgatg tagtcacgct
     6421 ttatcgtttt cacgaagctc tctgctattc cgttactctc cggactccgc accgccgtgt
     6481 tcttcggttc aagtcccaac atccgggcga actggcgtgt ttcattagcc cggtagcatg
     6541 aaccattatc cgtcagccac tccactggag acgacggaag atcgttgccg aagcggcgtt
     6601 ccaccgctcc cagcatgacg tcctgtactg tttcactgtt gaagccgccg gtagtgaccg
     6661 cccagtgcag tgcctcacga tcacagcagt ccagcgcgaa cgtgacacgc agtctctctc
     6721 cgttatcaca gcagaactcg aacccgtcag agcaccatcg ctgattgctt tctttcacgg
     6781 ccactctgcc tgtatgtgcc cgtttcgatg gcggtacagc aggttttcgc tcaagcaaca
     6841 gcgcattctg gcgcatgatc cggtaaacac gtttggcatt gatcgcaggc ataccatcaa
     6901 gttctgcctg tctgcgaagc agcgcccata cccgacgata accatacgtt ggcagctctc
     6961 cgataacatg gtgtatacgg agaagcacat ccgtatcatc agtgtgacga ctgcggcggc
     7021 catccatcca gtcatcggtt cgtctgagaa tgacgtgcaa ctgcgcacgc gacacccgga
     7081 gacaacggct gactaagctt actccccatc cccgggcaat aagggcgcgt gcgctatcca
     7141 cttttttgcc cgtcca
//
LOCUS       DAJOWB010000187         7113 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJOWB010000187 DAJOWB010000000
VERSION     DAJOWB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.187"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(205..690)
                     /gene="sixA"
                     /locus_tag="OHO85_004459"
     CDS             complement(205..690)
                     /gene="sixA"
                     /locus_tag="OHO85_004459"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCQ2673719.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(893..3037)
                     /gene="fadJ"
                     /locus_tag="OHO85_004460"
     CDS             complement(893..3037)
                     /gene="fadJ"
                     /locus_tag="OHO85_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCQ2673720.1"
                     /translation="MEMASVFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3037..4347)
                     /gene="fadI"
                     /locus_tag="OHO85_004461"
     CDS             complement(3037..4347)
                     /gene="fadI"
                     /locus_tag="OHO85_004461"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCQ2673721.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4527..4811)
                     /locus_tag="OHO85_004462"
     CDS             complement(4527..4811)
                     /locus_tag="OHO85_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCQ2673722.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5183..6523
                     /gene="fadL"
                     /locus_tag="OHO85_004463"
     CDS             5183..6523
                     /gene="fadL"
                     /locus_tag="OHO85_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCQ2673723.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6889..>7113
                     /locus_tag="OHO85_004464"
     CDS             6889..>7113
                     /locus_tag="OHO85_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673724.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVT"
BASE COUNT         1823 a         1885 c         1654 g         1751 t
ORIGIN      
        1 tgtcaaacag acattattca tttcaacatg taactttggg caaaatcaag atacgcgcag
       61 aagaataatt tccgaatgaa tgtagaaagt gttttattta tagataagga gagtcttcga
      121 tagtagtgag gttgacagat atccgctgcc tgatgagcaa ttatgcccaa tgaatctacc
      181 tcattgggca taatttggaa cacatcagat agcttttgcc atcttcagat tacacgggct
      241 catctgccag ttgaatgtac cgtttccgct ttcatcaagc gttacgctgg caatggccga
      301 ggtggtaaac ataggcggcg tttcgcccgg acataactcg gcaaccagat acccgactaa
      361 cggtaagtga gagatcacca gtaccgaagc aacaccttca ttggtcaacg cctgcaaata
      421 ggcactgacc agaccaacat cgccgcaggg cgttaactcc ggcagaactt ctgcactgga
      481 aggcaggttc agacaatccc ctacttcttc cagtgtttgc tcggctcgca ggaacgggct
      541 caccagaacg cgttcgattt ccactttttg acctttcagc cagttcgcca tcaggcgaga
      601 ttcgtcacaa ccattagtgg tcagaggacg aaccgaatca ctggcggcat cgagggctgc
      661 gtcgccgtga cgcatgataa aaacttgcat attgcaccgc ttttgttaac cagattcgcc
      721 cgttttcttt catctgaaac cagagaaaga aaacggtggc cggcattgtg ccttatccat
      781 tcaccgaatg aaacgctgtt ttttacctca atggcgtaag tatagtcaat atgcgtttac
      841 attttgacca acactccgcc attcagcgcg gattcatata gctttgacct tcttattgca
      901 ggtcagttgc agttgttttc caaaaacttt ccccacgcgc gcccatctcg accaaacgct
      961 cgcaaggggt aaaacgggaa ccatactgcg tggcaagtcg ttgcattatt gcaaccacct
     1021 cgcccgcgcc gagagaatcg atatagcgga acggtccacc gagaaatggc ggaaaaccaa
     1081 tgccaaatac cgcaccaata tccccgtcac gcacgctacg gataacctgc tcatcaacac
     1141 aacgtactgc ttcattcagc atcaacatca cacaccgttc agcaacctgc ggtgcggaga
     1201 gtcgcccctg cccttgtgcg ccaatgagcg ggtaaatggc gggatcgacc tgttttttgc
     1261 ttttacgccc tttctgacca taaagataga aaccccgacc attttttctg cctttgcgat
     1321 cgtcgttcaa aattgaagaa acaacatttg caggcgcgct aaaacgttct ccataagcgg
     1381 cttccagtac aggaataatt ttagtcccgg tgtcgattcc tacctcatcc aaaagttgga
     1441 ttggacctac cggaaaacca aatttcacta gcgcagcatc aatgtgctct acccgttcac
     1501 cttcggtcag catgcggata gcttcattaa tgtaaggcgc taagatgcga ttgacgtaaa
     1561 acccggcttt gtcacgcacg acaatgggcg ttttgccctg ttttttcgcc agttttaccg
     1621 tagtggcgat ggtttgcgct gatgtacccg catgaggaat aatctccacc agcggcattt
     1681 tttccaccgg actgaagaaa tgcaggccga taacttgctc aggtcgcgtg gcgtgagcgg
     1741 cgatatcacc aatcggcaaa gatgacgtat tcgaagcaaa gatggtatga gcggcgcaat
     1801 tttgctccac ttccgccacc atctgttgtt ttaattcgag attttcaaac accgcttcaa
     1861 taatcagatc gcgatgggca aagccgcgat agtccgtcgt tccggagatt aatgccagct
     1921 gtttgtcacg ttcgctggct ttgagatgac gacggcgaac tttgccctcc agctgatccc
     1981 aactgtactt cagcgcatga tttatgccct gcgggttgat atctttaatt ctgaccggaa
     2041 gccccgcttt acaagcagtg acataagcaa taccgccgcc catcaagcca ccacctaaaa
     2101 ttcccacgct gtttaatggc gcaggcggtg catcactacc gggatctttc ttcacttccg
     2161 tactggcaaa aaagatacta cgcaaagctt gcgattgtgg cgtcatcgcc agttcgccaa
     2221 acgcccgagc ttcggcgtca taaccgctgc tggtgccctg cgctaatccc gtttcaacaa
     2281 cctccaggat gcgttctgtc gccggataat tgccttgagt tttgtgttct gttttcttac
     2341 cgaccatttt gaacagcagc gcacgaccta acggccccgc cagaatacgc tcgcgtacag
     2401 gtagagggcg ggaagatggg cgatccttct tcgccagctc aacagcgact tccagcaaaa
     2461 tggagtgcgg aacaacgtca tccaccagcc ccagctttaa tgcctgtttc gcccgaagtt
     2521 gttttccggt gaggatcatc tctaatgctg tgctgacgcc tatcagacgc ggtaaacgct
     2581 gggtgccgcc tgaaccgggt aacaatccaa gttgtacttc aggcaaaccg agcaccgttt
     2641 taggatcgtc agtacaaacg cgaccgtggc acgccagcgc caactccagc ccaccaccca
     2701 ggcaagcgcc atgaatagcc gcgataaccg gaatgggcaa agcatgaatc tccgccatca
     2761 actgttggcc ctgccgcgcc agtgcttccg cttcttgcgc cgttttgcag ttgccgatca
     2821 tgttgatgtc tgcgccagca atgaagttgt ccggtttagc ggagacaaac accacgcctc
     2881 gcaactcttt gttttcacgg agttgcttaa taatggcgcg cacctgcgag gcaaactccg
     2941 ccttcagggt attcattttc tcacccggta cgtcgatggt gatgatagca atgttgtcca
     3001 gacgaacatt aagagtaaac actgatgcca tttccattat tccgcctcca gaaccattgc
     3061 cgcgccaagc ccaccggcag cacaggcggt aactaaacca aatccaccgc cgcgacggcg
     3121 aagttcatgc aatgtctggg taatcatccg cgcgccggtc gccgcgaagg gatgcccgta
     3181 agcaatcgaa ccgccaagca cgttaaattt gctatcgtcc acttcgccag tggcatgtgc
     3241 acgccccagt acttcacgag caaaacgttc actacccagc aactgaatat tcgccagtgt
     3301 ctgagctgca aaggcttcgt gcatatcgat caatgtcaga tcgctcattg tcaaaccggc
     3361 acgttccagc gccagcggtg ttgaccaggc cggaccgagc aacatgtcct gccagacatc
     3421 aatcgcagta aatgcgtagc tgcgcagata ccccagcggc accagcccta attctttcgc
     3481 ccgggattca gtcatcagga tcaccgctgc cgcgccatcg gtcagcggcg tactgtttgc
     3541 cgccgttacc gttccgtgtt tgcgatcaaa cgccgggcgc agctttgcgt aatcggcaag
     3601 cgaggaatta ccgcgaatat tgttgtcttc gacaagcggt tgtttataag gagggataaa
     3661 ggcagtcatc acctcttctt tgagttttcc gtctgaccat gcctgagcgg cacgctgatg
     3721 cgaacggtgc gctaatgcat cttgctgttc tcgggtgatg ccgtaggttt tcgccatttg
     3781 ctctgcggtg tcacccatcc gcaagccggt agaatattct gctactgcag gcggtacggg
     3841 cattaaatcg cgcaaacgca ggcgagagaa gagtttcagg cgctggctca tggttcgagc
     3901 tttgttgaca tcaaccagca cgcgcgccag ttttttactg acgccaattg gcaatacgga
     3961 agaggaatct gccccaccgg caatccccgc tcggatagtt cccgccatca ggctttctgc
     4021 gacgtttgca accgcctgga aactggtagc gcaagcgcgg ctgacgctgt aagcatcggt
     4081 atgcacattc attccggtac cgagaacaat ttcacgcgca atgttggggg cttcaggcat
     4141 ttgtacgacc tgaccaaaga ccagttgttc aatcacttcg gcggggatct cgctgcgtgc
     4201 cagcaattcg cctaccacca tcttccctaa atcaaccgcg ggaatgccat gaaaagccgt
     4261 cgcctgacgg gcaaaaggcg tacgtaaacc gctaacaatg gcgatacgat cgccctggcg
     4321 ggtaaccagc ggtaaaacct gacccataac actcccctgt aaaaaaataa ataaagtggt
     4381 ctgacctgat catagtctta accatttttt tacatttagc caagcggaga aaaaggaaag
     4441 tgggagctat gacacagtga agacaggaga aagaaaagaa aacgcccctg ccagcaactg
     4501 acaggggcga acgtacccga gaggaattaa cgcagaccca gctggaagat cagcatttca
     4561 gcttcgcagg caaaagtgaa atcgatatcc aggcgcacac cgtcagactc ttcagtgaaa
     4621 gtcggggaga ttttgcacgg ttcggattcc acgctacggg ctttttcagt cagcgccgcc
     4681 agcgtttgtt ctgcttctgc gcggtttgca aacacgcggc tgtaagacgc ggtgcagtcg
     4741 gagttgtcca taatggtgcc aacatccata cagcagcaaa ccggggtttc atcagcacta
     4801 catttactca tcgttgattt cctctgtatg tgcacccaag gtgccagata aacgttgtgg
     4861 atattttacg cttccggaaa gtgctgctcc agttgttaat tctgcaaaat cggataagtg
     4921 accgaaatca cacttaaaaa tgatctaaaa caaaattcac ccgaatccgc gagtgcgcaa
     4981 cctccaaatt ttgccagctg gatcgcgttt cttagatcat atttgaaaaa agatggaaac
     5041 atacttgcaa cattccagct ggtccgacct atactctcgc cactggtctg atttctaaga
     5101 tgtacctcag accctacact tcgcgctcct gttacagcac gtaacatagt ttgtataaaa
     5161 ataaatcatt gaggttatgg tcatgagcca gaaaaccctg tttacaaagt ctgctctcgc
     5221 agtcgcagtg gcacttatct ccacccaggc ctggtcggca ggctttcagt taaacgaatt
     5281 ttcttcctct ggcctgggcc gggcttattc aggggaaggc gcaattgccg atgatgcagg
     5341 taacgttagc cgtaaccccg cattgattac catgtttgac cgcccgacat tttctgcggg
     5401 tgcggtttat attgacccgg atgtgaatat cagcggaacg tctccgtctg gtcgtagcct
     5461 gaaagccgat aacatcgcgc ctacggcatg ggttccgaac atgcactttg ttgcaccgat
     5521 taacgaccaa tttggttggg gcgcttctat tacctctaac tatggtctgg caacagagtt
     5581 taatgatact tatgcaggcg gctctgtcgg gggtacaact gaccttgaaa ccatgaacct
     5641 gaacttaagc ggtgcgtatc gcttaaataa tgcatggagc tttggtcttg gtttcaacgc
     5701 cgtctacgct cgcgcgaaaa ttgaacgttt cgcaggcgat ctggggcagt tggttgctgg
     5761 tcagattatg caatctcctg ccgggcaaac tcctcaaggg caagcattgg cagctaccgc
     5821 caacggtatc gacagtaata ccaaaatcgc tcatctgaac ggtaaccagt ggggctttgg
     5881 ctggaacgcc ggaatcctgt atgaactgga taaaaataac cgctatgcac tgacctaccg
     5941 ttctgaagtg aaaattgact tcaaaggtaa ctacagcagc gatcttaatc gtgcgtttaa
     6001 taactacggt ttgccaattc ctaccgcgac aggtggcgca acgcaatcgg gttatctgac
     6061 gctgaacctg cctgaaatgt gggaagtgtc aggttataac cgtgttgatc cacagtgggc
     6121 gattcactat agcctggctt acaccagctg gagtcagttc cagcagctga aagcgacctc
     6181 aaccagtggc gacacgctgt tccagaaaca tgaaggcttt aaagatgctt accgcatcgc
     6241 gttgggtacc acttattact acgatgataa ctggaccttc cgtaccggta tcgcctttga
     6301 tgacagccca gttccggctc agaatcgttc tatctccatt ccggaccagg accgtttctg
     6361 gctgagtgca ggtacgactt acgcatttaa taaagatgct tcagtcgacg ttggtgtttc
     6421 ttatatgcac ggtcagagcg tgaaaattaa cgaaggccca taccagttcg agtctgaagg
     6481 taaagcctgg ctgttcggta ctaactttaa ctacgcgttc tgataacgcg ttcgcctgga
     6541 taaagtcacc tgcatagcag gtgactttaa ctcccccact tcaccgaaag tagtgtcctc
     6601 attgcttacc tcctgagttt tgcaaacagc ctgttggcag cttgcccttt caaatcaata
     6661 agcggtatcc acagacaaac ctgaagggaa aggcatattt tcaggcgttc tgctcgtcct
     6721 tctcaaagag tttacttttc tgcatttcca ggatactcaa cccccctggc tattgtgcgc
     6781 tcatacactc aaattaaaga taggttctaa ataaatgagc gttttttgat agtctatttc
     6841 attaggtaat atatatttgt aacaaatcaa tcaaaatgga ataaaatcat gctaccatct
     6901 atttcaatca acaataccag cgcaacttac ccagaatcca ttaatgaaaa taacaatgat
     6961 gaagttaatg gattagtaca agagctcaaa aaccttttta atggtaagga aggaataagc
     7021 acctgtatta aacatctact tgagcttata aaaaacgcca tacgagtaaa cgacgatcct
     7081 tatagattta atattaataa ttcctcagtt act
//
LOCUS       DAJOWB010000188         7084 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJOWB010000188 DAJOWB010000000
VERSION     DAJOWB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.188"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            556..1545
                     /gene="araF"
                     /locus_tag="OHO85_004465"
     CDS             556..1545
                     /gene="araF"
                     /locus_tag="OHO85_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HCQ2673725.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVLHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1615..3129
                     /gene="araG"
                     /locus_tag="OHO85_004466"
     CDS             1615..3129
                     /gene="araG"
                     /locus_tag="OHO85_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCQ2673726.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3144..4130
                     /gene="araH"
                     /locus_tag="OHO85_004467"
     CDS             3144..4130
                     /gene="araH"
                     /locus_tag="OHO85_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCQ2673727.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4297..5097
                     /gene="otsB"
                     /locus_tag="OHO85_004468"
     CDS             4297..5097
                     /gene="otsB"
                     /locus_tag="OHO85_004468"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCQ2673728.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5072..6496
                     /gene="otsA"
                     /locus_tag="OHO85_004469"
     CDS             5072..6496
                     /gene="otsA"
                     /locus_tag="OHO85_004469"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HCQ2673729.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGIVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6503..6931)
                     /gene="uspC"
                     /locus_tag="OHO85_004470"
     CDS             complement(6503..6931)
                     /gene="uspC"
                     /locus_tag="OHO85_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCQ2673730.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1798 a         1647 c         1831 g         1808 t
ORIGIN      
        1 gctttttgta tcttgttgat attaatcaat gaatttgaat tcatgatgaa gtgatgattt
       61 tataaaacgt tttttcattt ttgcgagatg gctctcatta tacgtgttct gttaattaat
      121 gagcacagtg ataattaatg acgaaatgaa tgacgtgcat tttccacatc tttgagttgc
      181 ggttattaca ccatttcaaa aactcaacgc caggtaatgc ggcctattga ctggttaaaa
      241 agaagacatc ccgcatgggt accaaagaca acaaggattt ccaggctaat cttatggatt
      301 aatctgctgt gcattcgaca atttgtctga caaattggct ttcccttatg tcttttcccg
      361 ctaaatttat gcacgttctc actgtaattc tgcgatgtga tattgctctc ctatggagaa
      421 ttaatttctc gctaaaacta tgtcaacaca gtcacttatc ttttagttaa aaggtaatgc
      481 tttgttttcc gattaattta acgaatgtca ttcgtttttg ccctacacaa aacgacacta
      541 aagctggaga gaaccatgca caaatttact aaagccctgg cagccattgg tctggcagcc
      601 gttatgtcac aatccgctat ggcggagaac ctgaagctcg gttttctggt gaagcaaccg
      661 gaagagccgt ggttccagac cgaatggaag tttgccgata aagccgggaa ggatttaggg
      721 tttgaggtta ttaagattgc cgtgccggat ggcgaaaaaa cattgaacgc gatcgacagc
      781 ctggctgcca gtggcgcaaa aggtttcgtt atttgtactc cggaccccaa actcggctct
      841 gccatcgtcg cgaaagcgcg tggctacgat atgaaagtca ttgccgtgga tgaccagttt
      901 gttaacgcca aaggtaagcc aatggatacc gttccgctgg tgatgatggc ggcgactaaa
      961 attggcgaac gtcagggcca ggaactgtat aaagagatgc agaaacgtgg ctgggatgtc
     1021 aaagaaagcg cggtgatggc gattaccgcc aacgaactgg ataccgcccg ccgccgtact
     1081 acgggatcta tggatgcgct gaaagcggcc ggattcccgg aaaaacaaat ttatcaggta
     1141 cctaccaaat ctaacgacat cccgggggca tttgacgctg ccaactcaat gctggttcta
     1201 catccggaag ttaaacattg gctgatcgtc ggtatgaacg acagcaccgt gctgggcggc
     1261 gtacgcgcga cggaaggtca gggctttaaa gcggccgata tcatcggcat tggcattaac
     1321 ggtgtggatg cggtgagcga actgtctaaa gcacaggcaa ccggcttcta cggttccctg
     1381 ctgccaagcc cggacgtaca tggctataaa tccagcgaaa tgctttacaa ctgggtagca
     1441 aaagacgttg aaccgccaaa atttaccgaa gttaccgacg tggtactgat cacgcgtgac
     1501 aactttaaag aagaactgga gaaaaaaggt ttaggcggta agtaatttgc cggaaaaatt
     1561 cccctctgca tgatgcagag ggggtgtgaa cgaccagtga ttcacggaga cgttatgcaa
     1621 cagtctaccc cgtatctctc atttcgcggc atcggtaaaa cgtttcccgg cgttaaggcg
     1681 ctgacggata ttagttttga ctgctatgcc ggtcaggttc atgcgttgat gggtgaaaat
     1741 ggcgcaggaa aatcaactct cttaaaaatc ctcagcggca actatgcgcc aaccacgggt
     1801 tctgtagtga ttaatgggca ggaaatgtcc ttttccgaca cgaccgcagc acttaacgcg
     1861 ggcgtggcga ttatttacca ggaactgcat ctcgtgccgg aaatgaccgt cgcggaaaac
     1921 atctatctcg gccagctgcc gcataaaggc ggcattgtga atcgctcatt gctgaattat
     1981 gaggcgggtt tacaacttaa acatcttggt atggatattg acccggacac gccgctgaaa
     2041 tatctctcca ttggtcagtg gcagatggtt gaaatcgcca aagcgctggc gcgtaacgcc
     2101 aaaattatcg cctttgatga gccaaccagc tccctctctg cccgtgaaat cgacaatctt
     2161 ttccgcgtta ttcgtgaact gcgaaaagag gggcgggtaa tcttatacgt ttctcaccgt
     2221 atggaagaaa tatttgccct cagcgatgcc attactgtct ttaaagatgg acgttatgtc
     2281 aaaaccttta ccgatatgca gcaggttgac cacgacgcgc tggtgcaggc gatggtcggg
     2341 cgcgacattg gcgatatcta cggctggcaa ccgcgtagtt atggcgagga gcgcctacgt
     2401 cttgatgctg tgaaagcacc aggcgtgcgt acgccaataa gtctggcggt tcgcagtggt
     2461 gaaattgttg ggctgtttgg tctggtaggg gcggggcgta gcgaattaat gaaaggcatg
     2521 tttggcggga cgcaaatcac cgccggtcag gtttatatcg accaacagcc gatcgatatt
     2581 cgtaaaccga gccacgccat tgccgcaggc atgatgctct gcccggaaga tcgcaaagcg
     2641 gaaggcatta ttcccgtgca ctccgttcgc gacaatatca acatcagtgc cagacgtaaa
     2701 catgtgctcg gcggttgtgt aatcaacaac ggttgggaag aaaacaatgc cgatcaccac
     2761 attcgttcgc tcaacatcaa aacgccgggc gcggagcaac tgatcatgaa tctctcaggc
     2821 ggaaatcagc aaaaagccat tctgggccgc tggttatcgg aagagatgaa ggtcattttg
     2881 ctggatgaac ctacgcgcgg cattgatgtt ggcgctaagc acgaaatata taacgtaatt
     2941 tatgcgctgg cggcgcaggg cgtggcggtg ctgtttgcct ccagcgactt acctgaagtc
     3001 ctcggcgttg ccgaccggat tgtggtgatg cgggaaggtg aaatcgccgg tgaattgtta
     3061 cacgagcagg cagatgagcg tcaggcactg agccttgcga tgcctaaagt cagccaggct
     3121 gttgcctgag taaggagagt atgatgtctt ctgtttctac atcggggtct ggcgcaccta
     3181 agtcgtcatt cagcttcggg cgtatctggg atcagtacgg catgctggtg gtgtttgcgg
     3241 tgctctttat cgcctgtgcc atttttgtcc caaattttgc caccttcatt aatatgaaag
     3301 ggttgggcct ggcaatttcc atgtcgggga tggtggcttg tggcatgttg ttctgcctcg
     3361 cttccggtga ctttgacctt tctgtcgcct ccgtaattgc ctgtgcgggt gtcaccacgg
     3421 cggtggttat taacctgact gaaagcctgt ggattggcgt ggcagcgggg ttgttgctgg
     3481 gcgttctctg tggcctggtc aatggctttg ttatcgccaa actgaaaata aatgctctga
     3541 tcacgacatt ggcaacgatg cagattgttc gaggtctggc gtacatcatt tcagacggta
     3601 aagcggtcgg tatcgaagat gaaagcttct ttgcccttgg ttacgccaac tggttcggtc
     3661 tgcctgcgcc aatctggctc accgtcgcgt gtctgattat ctttggtttg ctgctgaata
     3721 aaaccacctt tggtcgtaac accctggcga ttggcgggaa cgaagaggcc gcgcgtctgg
     3781 cgggtgtacc ggttgttcgc accaaaatta ttatctttgt tctctcaggt ctggtatcag
     3841 cgatagccgg aattattctg gcttcacgta tgaccagtgg gcagccaatg acgtcgattg
     3901 gttatgagct gattgttatc tccgcctgcg ttttaggtgg cgtttctctg aaaggtggca
     3961 tcggaaaaat ctcatatgtg gtggcgggta tcttaatttt aggcaccgtg gaaaacgcca
     4021 tgaacctgct taatatttct cctttcgcgc agtacgtggt tcgcggctta atcctgctgg
     4081 cagcggtgat cttcgaccgt tacaagcaaa aagcgaaacg cactgtctga tgcttttttc
     4141 tgcaacaatt tagcgttttt tcccaccata gccaaccgcc ataacggttg gctgttcttc
     4201 gttgcaaatg gcgacccccg tcacactgtc tatacttaca tgtctgtaaa gcgcgttctg
     4261 cgcaacacaa taagaaaaga gaaggaggag aaccgggtga cagaaccgtt aaccgaaacc
     4321 cctgaactat ccgcgaaata tgcctggttt tttgatcttg atggaacgct ggcggaaatc
     4381 aaaccgcatc ccgatcaggt cgtcgtgcct gacaatattc tgcaaggact acagctactg
     4441 gcaaccgcaa gtgatggtgc attggcattg atatcagggc gctcaatggt ggagcttgac
     4501 gcactggcaa aaccttatcg cttcccgtta gcgggcgtgc atggggcgga gcgccgtgac
     4561 atcaatggta aaacacatat cgttcatctg ccggatgcga ttgcgcgtga tattagcgtg
     4621 caactgcata cagtcatcgc tcagtatccc ggcgcggagc tggaggcgaa agggatggct
     4681 tttgcgctgc attatcgtca ggctccgcag catgaagacg cattaatgac attagcgcaa
     4741 cgtattactc agatctggcc acaaatggcg ttacagcagg gaaagtgtgt tgtcgagatc
     4801 aaaccgagag gtaccagtaa aggtgaggca attgcagctt ttatgcagga agctcccttt
     4861 atcgggcgaa cgcccgtatt tctgggcgat gatttaaccg atgaatctgg cttcgcagtc
     4921 gttaaccgac tgggcggaat gtcagtaaaa attggcacag gtgcaactca ggcatcatgg
     4981 cgactggcgg gtgtgccgga tgtctggagc tggcttgaaa tgataaccac cgcattacaa
     5041 caaaaaagag aaaataacag gagtgatgac tatgagtcgt ttagtcgtag tatctaaccg
     5101 gattgcacca ccagacgagc acgccgctag tgccggtggc cttgccgttg gcatactggg
     5161 ggcactgaaa gccgcaggcg gactgtggtt tggctggagt ggtgaaacag ggaatgagga
     5221 tcagccgcta aaaaaggtga aaaaaggtaa cattacgtgg gcctctttta acctcagcga
     5281 acaggacctt gacgaatact acaaccaatt ctccaatgcc gttctctggc ccgcttttca
     5341 ttatcggctc gatctggtgc aatttcagcg tcctgcctgg gacggctatc tacgcgtaaa
     5401 tgcgttgctg gcagataaat tactgccgct gttgcaagac gatgacatta tctggatcca
     5461 cgattatcac ctgttgccat ttgcgcatga attacgcaaa cggggagtga ataatcgcat
     5521 tggtttcttt ctgcatattc ctttcccgac accggaaatc ttcaacgcgc tgccgacata
     5581 tgacaccttg cttgaacagc tttgtgatta tgatttgctg ggtttccaga cagaaaacga
     5641 tcgtctggcg ttcctggatt gtctttctaa cctgacccgc gtcacgacac gtagcgcaaa
     5701 aagccataca gcctggggca aagcatttcg aacagaagtc tacccgatcg gcattgaacc
     5761 gaaagaaata gccaaacagg ctgccgggcc actgccgcca aaactggcgc aacttaaagc
     5821 ggaactgaaa aacgtacaaa atatcttttc tgtcgaacgg ctggattatt ccaaaggttt
     5881 gccagagcgt tttctcgcct atgaagcgtt gctggaaaaa tatccgcagc atcatggtaa
     5941 aattcgttat acccagattg caccaacgtc gcgtggtgat gtgcaagcct atcaggatat
     6001 tcgtcatcag ctcgaaaatg aagctggacg aattaatggt aaatacgggc aattaggctg
     6061 gacgccgctt tattatttga atcagcattt tgaccgtaaa ttactgatga aaatattccg
     6121 ctactctgac gtgggcatag tgacgccact gcgtgacggg atgaacctgg tagcaaaaga
     6181 gtatgttgct gctcaggacc cagccaatcc gggcgttctt gttctttcgc aatttgcggg
     6241 agcggcaaac gagttaacgt cggcgttaat tgttaacccc tacgatcgtg acgaagttgc
     6301 agctgcgctg gatcgtgcat tgactatgtc gctggcggaa cgtatttccc gtcatgcaga
     6361 aatgctggac gttatcgtga aaaacgatat taaccactgg caggagtgct tcattagcga
     6421 cctaaagcag atagttccgc gaagcgcgga aagccagcag cgcgataaag ttgctacctt
     6481 tccaaagctt gcgtaggagc ggttaatctc ccgtaagtgg aactaacagc acatcgacct
     6541 cacttgaggc aataactctt ttcgccgagc aggatgctcg cgaaaagaaa ctgtgattgt
     6601 gattaccgca aatcaccaaa tcgaaatgat gcttgtgaca tacttccaga atatgttcgc
     6661 ttaactcacc gtaggcaata aacgtcttgt caacgggata ccccgcatcc tgaattaact
     6721 tatcaagaaa gctttgcgtt tcttcctgca tcacgctgcg taaatcttca agcatcggcg
     6781 cagctaattg attgtacatt tccgggtcgg aagcgagagt aattaaactg atgtgtccct
     6841 ttactggcct ggcgatagat accgcttttg ccagcagttg ctgactttcc ggtgtaaccg
     6901 caacagcgac aagaatattg ctatagctca taacctgttc cttattctgt gaacttcagg
     6961 tgacattaaa gctttactcc taatggagaa acgacgcaat cccaactcgg tcaaacaggt
     7021 aaatattagt ctgattatta gtaaagacaa ttaatcaatc actcccggtg atcatataat
     7081 ttca
//
LOCUS       DAJOWB010000189         7028 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJOWB010000189 DAJOWB010000000
VERSION     DAJOWB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.189"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(271..888)
                     /gene="tdk"
                     /locus_tag="OHO85_004471"
     CDS             complement(271..888)
                     /gene="tdk"
                     /locus_tag="OHO85_004471"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCQ2673731.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFAEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1492..1905
                     /gene="hns"
                     /locus_tag="OHO85_004472"
     CDS             1492..1905
                     /gene="hns"
                     /locus_tag="OHO85_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCQ2673732.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2049..2957)
                     /gene="galU"
                     /locus_tag="OHO85_004473"
     CDS             complement(2049..2957)
                     /gene="galU"
                     /locus_tag="OHO85_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCQ2673733.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3159..4172)
                     /gene="rssB"
                     /locus_tag="OHO85_004474"
     CDS             complement(3159..4172)
                     /gene="rssB"
                     /locus_tag="OHO85_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCQ2673734.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4264..5169)
                     /gene="rssA"
                     /locus_tag="OHO85_004475"
     CDS             complement(4264..5169)
                     /gene="rssA"
                     /locus_tag="OHO85_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCQ2673735.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            5282..5740
                     /locus_tag="OHO85_004476"
     CDS             5282..5740
                     /locus_tag="OHO85_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCQ2673736.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5790..6632
                     /gene="purU"
                     /locus_tag="OHO85_004477"
     CDS             5790..6632
                     /gene="purU"
                     /locus_tag="OHO85_004477"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCQ2673737.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6792..6876
                     /locus_tag="OHO85_004478"
     tRNA            6792..6876
                     /locus_tag="OHO85_004478"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6826..6828,aa:Tyr,seq:gta)
     gene            6911..6995
                     /locus_tag="OHO85_004479"
     tRNA            6911..6995
                     /locus_tag="OHO85_004479"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6945..6947,aa:Tyr,seq:gta)
BASE COUNT         1908 a         1732 c         1533 g         1855 t
ORIGIN      
        1 ccttctgggg ccgttttttt gtttacccaa agcaactttt ccataaaccg acagcattag
       61 ccttcatcat atttgcgacg atgtataacg cgtaaacaca gggatattgt actttacagg
      121 tcacaagtca acgtcggtgc ttaagagccc tgtgaggcgt atagcggcgt taaaaaactg
      181 ccgagaaggg tgtatagccc ggaagaagtg cgtaaaacga actgacagga taaaagtgcc
      241 ctgctcaccc tgtcagtaaa gaaattctta ttaatcgtgg cgatgccttt cctgaatagc
      301 cgttaatgag ccgacttgta acgcctcttt atagtgttta cggcatacag aaacgtagcg
      361 ttcattacca ccaataacca cctgctcacc ttcgttataa ggtctgcctg cttgatcaag
      421 acgcagcacc atgcttgctt tacggccaca aaaacagatg gtttttaatt caaccagttt
      481 gtcggaccat gccagtaagt attggctacc aataaataat tcacctcgaa aatcggtacg
      541 taaaccataa caaagtacgg gtatatcgag ttgatctaca acctccgata attcatatac
      601 ttgttgtctg gttaaaaact ggcattcatc aaccagtacg caatgaattg cctgctgttc
      661 atgttccgca cgaatctcag caaataatga tgaattttgg ttaaataatt ttgcaggcga
      721 tgacaaacct atacgcgagc tgactttccc ggcaccaaag cgatcatcaa tttctgccgt
      781 atatacgaca gtgcgcatgc cgcgttcctg gtaattgtat gaagattgca acaatgctgt
      841 agacttaccc gcattcattg cggaatagta gaaatatagc tgtgccatca gccacaggcc
      901 ctcaatgata tgaaataacg ttggctggag tttatcataa ttcgtcagtt tttcagctca
      961 acagtatgca cagaagtggt atatgcgttc tcccttacga agccttgcat aatccttctg
     1021 agctatcatt actattgcct cgcttgttat aagcgggtaa atgactgctg gtaactattc
     1081 acaatcttta acctgttgcg caagtaatag ccctctgttg acctccagga gatagtgcaa
     1141 tactaagtcc ctgctcttat tgcgactgtt ctacttttca tcattcgctt aatagggaat
     1201 tctcgtaaac acgactaata cagaagactg aaaggtcgtc agcctacgat aatctcccca
     1261 taaaatgtga catgaatcag gaagttttaa cctcacgagc tgcgaaatca tcggtgcaaa
     1321 tagggctata tgccgcgtct tttctggcta attttatgaa aagatattta ttggcggcac
     1381 aaaataaaga acaattttga attccttaca ttcctggcta ttgcacaact gaatttaagg
     1441 ctctattatt acctcaacaa accaccccaa tataagtttg agattactac aatgagcgaa
     1501 gcacttaaaa ttctgaacaa catccgtact cttcgtgcgc aggcaagaga atgtacactt
     1561 gaaacgctgg aagaaatgct ggaaaaatta gaagttgttg ttaacgaacg tcgcgaagaa
     1621 gaaagcgcgg ctgctgctga agttgaagag cgcactcgta aactgcagca atatcgcgaa
     1681 atgctgatcg ctgacggtat tgacccgaac gaactgctga atagccttgc tgccgttaaa
     1741 tctggcacca aagctaaacg tgctcagcgt ccggcaaaat atagctacgt tgacgaaaac
     1801 ggcgaaacta aaacctggac tggccagggc cgtactccag ctgtaatcaa aaaagcaatg
     1861 gatgagcaag gtaaatccct cgacgatttc ctgatcaagc aataatcttt tgtagattgt
     1921 acttgcttaa aatcccgcca tcggcgggat tttttattgt ccgatttaag acaatttaat
     1981 aagaataagc tataaaaaaa cggcgtcgat tgctcaacgc cgtttcgtgg ataacaccga
     2041 tacggatgtt acttcttaat gcccatctct tcttcaagcc aggctttaaa ttccgtgcca
     2101 agggtgttat gacgaatacc gtattcaacg aaggcctgca tgtaacctaa tttattaccg
     2161 cagtcatggc tcttcccttt catatgatag gcttccaccg tttctttttc gatcagcata
     2221 tcaattgcgt cggtgagctg aatttcatca ccagctccgg gaggggtttt tgccagcaac
     2281 ggccaaatat ccgcgctaag tacgtaacga cccacaatag cgagattaga cggcgcaaca
     2341 tccgcttttg gtttttcaac cacgccaacc atcggtacgc tttcacccgg cgctaattca
     2401 acgcctttgc aatccacaac gccatatgcg gtcacatcag caaccggttc aaccatgatc
     2461 tggctatgac ccgtttcatc aaagcggcgg atcatctctg ccaggttatc ctgtgacaaa
     2521 tcggattcat attcatccag aataacgtca ggcaaaataa cagccacagg ttcatcaccc
     2581 actaccgggt gagcacacaa taccgcgtgt cccaggcctt tcgccagccc ctgacgaact
     2641 tgcataatag tcacgtgcgg cggacaaata gactgcactt catcaagcag ttgacgtttt
     2701 acacgttttt ccagcattgc ttccagttca aaactggtat caaagtggtt ttcaatagag
     2761 tttttagatg agtgtgtaac cagcacaatt tcagtaatgc cagccgcaat acattcattc
     2821 acgacgtatt gaattaatgg cttatcgaca agtggcagca tctctttcgg gatggctttc
     2881 gtcgccggca acatcctggt tcctaatccc gcaacgggga taacggcttt tttgactttc
     2941 gtattaatgg cagccattta aattctcctg gactgttcgt gttttgaacg tgttcatatt
     3001 tctgtatcgc atcccagtat atcagcaccc cgaaggggct aagttctgaa aaggaggcga
     3061 gttagcataa ttaagacaat tacttatacg aatggcgacg atagtaccac cagccgacat
     3121 tagcaggtaa tgcaaattta gcccgcgtta tcgtttgctc attctgcaga caacatcaag
     3181 cgcagtcgac caccggttcc ccatatttgg cattgccagg catcgcatcg ctggctcaat
     3241 tgattcaaat aagcgttacc taaagtgcct aacggaacac cattactgat ttgcacctgg
     3301 tgttcgccgg tatttaacgt cgcattcaga cccgcagaaa ccagaatgag atttttcagt
     3361 tcgcgatgat aatagccaac taatagcgga aactgccccg gcagattggc ctgacggagt
     3421 aaatggttta cctgcttcaa taacgcgccc aactctggca accgttgatt ttggtgtgca
     3481 agctgttcct gtaataatcc gttaaacaat gcgcgtaata acaaggcagc aagtacgcca
     3541 ttatgtccag ctcgggttac atcaaggcaa taaaatgcca gatcgttttc cgaaagtgcg
     3601 gcaatatcaa gcaccaggcc gggtttgtcc gcggcaacca attgacgata attaacccgg
     3661 caatgggaaa tcacctgctg aaccggcggt tgtagttcct gtaataattt cgccgctgcg
     3721 gcagggttat caaccattgc atcccagtcg cgaaaaagcc tttcctcttc ctcaacgcgc
     3781 gaattaaaca tgctgggata gagacaggca aaaaccatct cgcgcaagcg attcagatct
     3841 ttaactggtt tcagcaaaac atcttcaacg cccagacgta acgctttggc aatatctgcc
     3901 atattttcag tggcagatat caccagaact ggggtctggt cgcctctgtt acgtatatgc
     3961 tccagcagtt taagcccgtt cattcgtggc atcgcgatat cacatatcat caggtctgga
     4021 gtgaaacctc ccagcaactc aagggcatcc accccatcag ccgccagtac cgttgtcgct
     4081 cccaatgagg aaaaccacga atccagaagc gagcgaaata cctgctcatc ttcaacaatg
     4141 agaatctgtt ttccgaccaa tggctgcgtc atgttctctc ccctgactgg ctttactcaa
     4201 tagtggcatg ctattgccac ctgcgcctgt cagaattaac ttaagtgtag ataaaaattc
     4261 tggtcaaatg ttggtgcgta ccaacggcaa aagttcgtcc attttctttt ccactgccag
     4321 ctgtccggcc gcaatggcag cgtgcgcgcg atggaaatca agcgtagata tttgcgggca
     4381 aacaggttga atcagaatat cgggcggatc acctgccatg cggttccttt taaggcggtt
     4441 ctccagcacc tggatagaag tggtcataat ctctgttgcc gttggtgctg tcaccgcccg
     4501 acgtgtcgtt atgctgccta acctttcttt cagacgcgca tgccacggca gagaatcacc
     4561 attctcgcta ttttcttcac tgacattaaa ggagagcaaa tcttgttgca tcaaatgagc
     4621 atcgtgctgc aggtcgaccg ctatcacaat atcagcaccc aatgcacgcg tgagtgaaat
     4681 aggaattggg ttaacgactg ctccatcaac cagccagtag ccgttatgtg caacaggtgc
     4741 catgagtcct ggaatactgc atgatgcgcg aatagcaaga tggagatcgc cttcagtaaa
     4801 ccataattca cgtcccgtac ttaaattggt ggcaacagcc gcaaagcgac gggaacaatt
     4861 ttcgatctct gtttccggca ttatttcgcg gtattgattg aagacacgct cgccgcgcag
     4921 taacccaccg cgctgccagg agagatccat caggcgtaaa acgtcccaat aactgaaaga
     4981 ggttacccaa tcttccagcg cagataatcg atcgcatgca taggccgcgc ccaccagcga
     5041 accaattgaa catcctgcaa cgatatcaat ttcaataccc acttttttta gcgcattaat
     5101 aacgccaata tgcgaccaac ctctcgccgc gccagatccc agcgccagcc ctatttttat
     5161 ctttctcatt attcccgcaa gattcccctg gaatgcaatc gtagccacat cgctactgct
     5221 cggttaacat agtgctactc aggcggttaa tacgccgtta ttgtttccag ggagattatt
     5281 tgtgtctcag ctttgtccct gtggtagtgc tgtcgagtat agcctatgtt gccaccctta
     5341 tgtgtctggt gaaaaggttg cacctgatcc agaacatctc atgcgttcgc gttactgcgc
     5401 ttttgtgatg caagacgcag attatttaat aaagacctgg catccctctt gtggagcagc
     5461 ggcgttacgt gccgaattga tggccggatt tgcgcatact gaatggttgg gattgaccgt
     5521 atttgaacat tgttggcagg acgccgacaa tatcggattt gtcagttttg ttgctcgatt
     5581 taccgaaggt ggcaaaacag gtgcaataat tgaacgttcg cgatttttga aagaaaacgg
     5641 tcaatggtac tatattgacg gtacacgtcc gcagtttggt cgcaacgatc cctgcccttg
     5701 tggttcaggt aaaaaattta aaaagtgctg cggccaataa tggttgacgg tacggtttag
     5761 caaacactct caacaaggtt tttccagcaa tgcattcact ccaacgtaaa gttctgcgta
     5821 ctatttgtcc ggaccaaaaa ggtctgatcg cacgtattac caatatttgc tacaagcacg
     5881 agttaaatat cgtacagaac aatgaatttg ttgatcaccg taccgggcgc ttttttatgc
     5941 gcacggaact ggaagggatt tttaatgatt ccacccttct ggcggatctc gatagcgcat
     6001 tgccagaagg ctccgtgcgt gagctgaatc ctgccggtcg tcgccggata gtgattctgg
     6061 tcactaaaga agcgcattgc cttggcgatt tgttgatgaa agccaattac ggcggcctgg
     6121 atgtcgaaat cgcggcagtt attggtaacc acgatacttt acgttctctg gttgagcgtt
     6181 ttgatattcc gtttgagctg gtaagccatg aagggttaag ccgcaacgag cacgatcaaa
     6241 agatggcgga cgccattgat gcttatcaac ctgactatgt ggtgctggcg aagtatatgc
     6301 gggtattaac gccggaattt gtggcacgct tcccgaataa aatcatcaat attcaccatt
     6361 cattcctgcc tgcgtttatc ggcgcacgtc cttatcacca ggcctatgaa cgtggtgtga
     6421 agattattgg cgcaaccgct cactatgtga atgacaatct ggacgaaggc ccaatcatca
     6481 tgcaggacgt tattcatgtc gatcatacct acacagctga agatatgatg cgcgcaggtc
     6541 gtgacgtcga gaaaaacgtc ttaagtcgtg cgctatacaa agtactggca cagcgcgtct
     6601 ttgtttacgg taatcggacg attattcttt aatcgccagc aaaaataact ggttaccttt
     6661 aatccgttac ggatgaaaat tacgcaacca gttcattttt ctcaacgtaa cactttacag
     6721 cggcgcgtca tttgatatga tgcgccccgc ttcccgataa gggagcaggc cagtaaaaag
     6781 cattaccccg tggtggggtt cccgagcggc caaagggagc agactgtaaa tctgccgtca
     6841 tcgacttcga aggttcgaat ccttccccca ccaccatctc aagcattacc tcaaaatttg
     6901 aattacccct ggtggggttc ccgagcggcc aaagggagca gactgtaaat ctgccgtcat
     6961 cgacttcgaa ggttcgaatc cttcccccac caccatcact ttcaaaagtc cctgaactct
     7021 caaacgaa
//
LOCUS       DAJOWB010000190         6868 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJOWB010000190 DAJOWB010000000
VERSION     DAJOWB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.190"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(103..618)
                     /gene="traV"
                     /locus_tag="OHO85_004480"
     CDS             complement(103..618)
                     /gene="traV"
                     /locus_tag="OHO85_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HCQ2673738.1"
                     /translation="MKQISFFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPL
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(615..866)
                     /gene="trbG"
                     /locus_tag="OHO85_004481"
     CDS             complement(615..866)
                     /gene="trbG"
                     /locus_tag="OHO85_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HCQ2673739.1"
                     /translation="MNKLVSDSSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRSTVFENPDIVKTCTVRDLKKDFVNCDEKGEGQ"
     gene            complement(878..1108)
                     /locus_tag="OHO85_004482"
     CDS             complement(878..1108)
                     /locus_tag="OHO85_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HCQ2673740.1"
                     /translation="MTEQTETTEIKMNMRNINVITALSVPGKTVSDDFMHAVLSNCAT
                     RIVLPAPEKFGSESLPDNFNMTAVGVMKNSEI"
     gene            complement(1062..1652)
                     /locus_tag="OHO85_004483"
     CDS             complement(1062..1652)
                     /locus_tag="OHO85_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HCQ2673741.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVRPGKMKCIRVVTADEHLK
                     EVYTEDASLPGKIRKCPVYLPDDRTNRNNGDKNEHA"
     gene            complement(1642..3069)
                     /locus_tag="OHO85_004484"
     CDS             complement(1642..3069)
                     /locus_tag="OHO85_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HCQ2673742.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(3069..3773)
                     /gene="traK"
                     /locus_tag="OHO85_004485"
     CDS             complement(3069..3773)
                     /gene="traK"
                     /locus_tag="OHO85_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HCQ2673743.1"
                     /translation="MFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAISNTSPNLFT
                     VPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLSIQAVPREG
                     AGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQVPVTKETLQ
                     APAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMFSQPASQLL
                     AGARMDVYVIRDGEGN"
     gene            complement(3784..4350)
                     /gene="traE"
                     /locus_tag="OHO85_004486"
     CDS             complement(3784..4350)
                     /gene="traE"
                     /locus_tag="OHO85_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HCQ2673744.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNIIQGVNNYRLQNE
                     QRTAVTPMAFNSPFAVSQNSADASYLQQMALSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(4372..4683)
                     /gene="traL"
                     /locus_tag="OHO85_004487"
     CDS             complement(4372..4683)
                     /gene="traL"
                     /locus_tag="OHO85_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HCQ2673745.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(4698..5063)
                     /gene="traA"
                     /locus_tag="OHO85_004488"
     CDS             complement(4698..5063)
                     /gene="traA"
                     /locus_tag="OHO85_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HCQ2673746.1"
                     /translation="MDAVLSVQGASAPVKKKSFFSKFTRLNMLLLARAVIPAAVLMMF
                     FSQLAMAAGSSGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFL
                     AGFAIISVFIAVGMAVVGL"
     gene            complement(5097..5324)
                     /gene="traY"
                     /locus_tag="OHO85_004489"
     CDS             complement(5097..5324)
                     /gene="traY"
                     /locus_tag="OHO85_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HCQ2673747.1"
                     /translation="MRRRNARGGVSRTVSVYLDEDTNNRLIKAKDRSGRSKTIEVQIR
                     LRDHLKRFPDFYNEEIFREVTEESESTFKEL"
     gene            complement(5418..6104)
                     /locus_tag="OHO85_004490"
     CDS             complement(5418..6104)
                     /locus_tag="OHO85_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HCQ2673748.1"
                     /translation="MCALDRRERPLNSQSVNKYILNVQNIYRNSPVPVCVRNKNRKIL
                     YANGAFIELFSREDKPLSGESYIRLQVEIFLSSLELECQALGHGSAFCRRFNFHGEIY
                     QIRMENVSFYNDESVVLWQINPFPDYPFFALNQSVSNTNTSGQLTIWNDLSPGTLVVF
                     SFYMLGVGHAIIARELGITDRASEDRIKPVKRKIKEFFEHFDLFRVSCIYKGEIDSLL
                     SIIREFYGVK"
     gene            complement(6294..6677)
                     /locus_tag="OHO85_004491"
     CDS             complement(6294..6677)
                     /locus_tag="OHO85_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HCQ2673749.1"
                     /translation="MARVNLYISNEVHEKINMIVEKRRQEGARDKDISLSGTASMLLE
                     LGLRVYDAQMERKESAFNQTEFNKLLLECVVKTQSTVAKILGIESLSPHVSGNPKFEY
                     ASMVDDIREKVSVEMDRFFPKNDDE"
BASE COUNT         1748 a         1807 c         1524 g         1789 t
ORIGIN      
        1 accgcccatc ccttttcacg cacagggcac tcgttttaca ggcagatttt gaatacagaa
       61 agcccgccgg cattgccggc tgatatacaa cttcagggcc agttaattaa tacgtggttt
      121 tccccacgca gacggtttaa taacaaaaaa cacgctggat ggctgatgat aaacatcctg
      181 gttatcaata taaggcgcta tccataatgc tgccgtctgc tcacctgttc ttagcggacg
      241 aggaggtgtt accggcgtaa ctgaactgac cggaacaacc gttttcactt cccgggcagc
      301 ggtaaacagc gggagaggtg ccagcagttt ctgctccgga tgtgctgtca ccgcaggtct
      361 gctgccggac ggagtggtcg cggtgacggt ctgcaccggc attgtccgga agttcccttc
      421 agccaggcgc ggcagtgatg ccgcaaccgg ctttgcttct gaggactgct ccagtttttt
      481 ggccttctca ttggcctgct ccatcgtcat acaggtatcg gatgtggtgg cgttacactc
      541 aaattccgta ctggttccgg cacagccgga aagtaataag gtccccagca gaggaataaa
      601 gaaagaaatc tgtttcattg tccctcacct ttctcatcgc agttcacaaa atccttcttc
      661 aaatccctga ccgtacaggt ttttacaatg tcaggatttt caaaaaccgt actccgtgat
      721 gatttataaa catatgccac aatgcgtttc ccacccgcat caggttctgg tgcactgact
      781 tcacataacg gaggtgttat tccggactca tacagcaccg gataatttat ttttttgacg
      841 ctgctatcag aaaccagctt attcatcgaa gggttcctta tatttctgaa ttcttcatca
      901 caccgacagc agtcatatta aaattgtccg gcaatgactc agaaccgaat ttctccggtg
      961 caggaagtac gattcttgtg gcgcagttac tgagaacagc atgcataaaa tcatctgaca
     1021 cggttttacc cggcacagag agggctgtaa taacattaat attacgcatg ttcattttta
     1081 tctccgttgt ttctgtttgt tctgtcatca ggaagataaa cagggcattt cctgattttt
     1141 cccggcaggg aagcgtcttc cgtataaact tccttcagat gttcatctgc ggttaccacc
     1201 cggatacatt tcatttttcc gggtcgcacc ggatatttcc cggtgatcgc tgccagcgag
     1261 acatacatca gtgccagtgt tgcccataat gcggtgacca gttgcatcag tgaccggtca
     1321 gtctccctga cataatctgc agcatcagcc gcgcggtaat ggcagatatc ctgtatttct
     1381 gatctgcctg aagaatcaga ggaagaaaac cagcattcac tgaccgggaa ctgtccggca
     1441 ggtgctgttt gtcttacgaa gttaatctcc ttaaccagta atttccccgg cgtggtgttg
     1501 tccttccata aaacaaacag agccatcagg gccattgtgg ccagtggcag aatgacggtg
     1561 tacttcagac accagaacag tcctcgcaga acccgggcga caacataccg ggcggcatgg
     1621 cacgcctgac gggacgacat attatttgcc atcgttgccc cctccctggc tgccggacat
     1681 aaactgctgc ggattcaggt tgcccagctg cttcagcatc tggtcagtat taaagccgtt
     1741 aagatgactt tcagccgatg gcggaacagg aaccggactt tccggattgt cttcttccgc
     1801 tctgctctgc gtgcgttcca gcgccatctc ttccacggtt ttcagctgga agccgtcctg
     1861 gaacaccacg gtcacttcgt tgcccgcacc aatcggtatc accgggtgat actgttcggc
     1921 acgtttgatg tagtagtcac tgagcgtctg tgcggctttc gatgcgccgc caccgatacc
     1981 cattttcagg acatcaccag ccccgtaagc ggctgtggca cccagcccga cagccggctg
     2041 ggaggcgcgc tccattccct gaccgatacc gtcaacaaat cccgcgcccc atgcccagcc
     2101 gaggattttg ccgttacgca tcaccacttc gcccttgata ccgtttttac cccggaagct
     2161 gacatgcccc ttaatcggca tatcaatagt tttgccgtcc ttcaggcaac tgatattgcg
     2221 ggtgcgtaca atggcacgct cactggacac atccccccag gcttccagac ccacaaaaca
     2281 tcccgttgcg tcatacgtct tgctgttcgg catttccacc aggccggtga tacgcagctg
     2341 catcggcacc gtggattcat taccggtgac agaggcattg gcatccgccc cttcaatcag
     2401 catggctttc gcaaaacttc ctgacggaat gtacggcagt gatggtccct gttttccctc
     2461 attacgggta aacaccttac gctgtatccg gttaggcacc ggcacagact ggtacgtcac
     2521 ctgtggtggt ggcgtgacac cattcccggg ataaaacgct gtcggcggtg gaacagcaac
     2581 gctgccctgc ggcgggaagg atacgggggt gtttcctggc tgtggttcgc cttccgggcc
     2641 gggcggtgag gcaggcatct gcggtacagg ctcaccggtc gccgtgacgg gattagcacc
     2701 cagggctttt acctgctctg ccagggcggc attgtcctgt cccaactttt caatacgacg
     2761 ctggtcatca ccgcgctgtt tgttcagaac atccagctca cgacggattt cctcatactg
     2821 cttctgcatc tgcgctgccg tcacctgcat ctcggtggtg gcatgctgac gcaccttgtc
     2881 atcaaaggtc gtatccacca caccggtcat atccggcaca ggctcctgtt cagccacggt
     2941 ttcaccgtta ccggacatgt ccacatcaga cagatacagt gccccaccaa tcgcggaggc
     3001 tgtaccgaca accacaatcc ccagccacag gtactgcttg cgtttcacaa tggtattgat
     3061 actggccatc agttgccctc cccgtcacgg atgacataca catccatgcg cgcacctgcc
     3121 agtaactggc tggcaggctg gctgaacatc acggcacggg ttcccggctg ccagaagtca
     3181 ctttcccgga tattcagggc agacagcgtt ttattttcca cggcaaagcg gaccatcttc
     3241 aggtgattcc ccgtccatac ggcatctgcc actgaagaca gccccgccgg ggcctgcagg
     3301 gtttcctttg tcactgggac ctgataccag cctgcgggta attttccgcc acggacggcc
     3361 tggctgatgg tcacaagcag ggattcgtaa ggcgtggacg tttcccacgc accggcttct
     3421 tctccggttc cgcgcaggtc actgaccagc tgaatggtac gccccgcgcc ttcacgggga
     3481 acggcctgaa tggaaagatt cagaccacgt tctgtttcca gaatgaacgt aaagggcttt
     3541 ttgttgacgg tggcaaccac cacaccgccg gaggcggtct gctcattatt ggtcagggca
     3601 ccatccagac tgttcacggc gataatccgg tcaccgggaa cggtaaacag attcgggctg
     3661 gtattgctga tggcaatact ggcctgacca ccgttcacca ttggcaccac ggtgggggcc
     3721 agcgtaccgt ttgcggccat cacgctgcag gaaaaaaaca ggctgccgaa tattattgcc
     3781 gtattatttt ttctcatcgt ctgtttcccc gaaattatcc agccaggtca ccccgttttc
     3841 ccgcttcaga ataaggatgt aatgtttgat atcagtgaaa ggttttgaat caccaatcca
     3901 ggttttaagc acacctcgaa tttccacacg gccatactga ggccagacac gaacactggt
     3961 ctggaaaaag gctgagttca cgttatcgtt tttaatacgc ttcgcttctt cagccagaat
     4021 aactttcatc tggttctgtg cgcccgggcg gatatattgc agaagcgcct gatgtgaggc
     4081 atcgacggtt tctgatgaaa cattcagacg gagggcaata aatgacagcg ccatctgctg
     4141 taaataagag gcgtcggcac tgttctgtga cacggcaaag ggggaattaa atgccattgg
     4201 cgtcacggca gtgcgttgct cattctgaag acggtagtta ttcaccccct gaatgatgtt
     4261 aacagagagg ctgagaacaa taagcactga cataaatata aaggcgatgg ccattacacg
     4321 actggtactt aaacgggcac cgtgttccat ataacaatcc tggtatcagt tctatttaat
     4381 ccactgccgg aaacacgaat cgggaacatt atgaaaaata ccgcgcagca gggctgttgg
     4441 catataccag taaatcaggt cacgtaacca ggaactgccc cgcccttttt tcagtttttt
     4501 aatcccgaaa taaaccagaa ccgctgcacc aataccgaac agatatttcg atgttgtgat
     4561 accccagcca atacagattg ctgcggggat cagttcatcc agtggcaggc caaaccagcg
     4621 gctctggttg gtcagtgttt ccgggaaacg atatttctta agtttattct cgtctcccga
     4681 catcgtttta tttcctgtca gaggccaacg acggccatac ccacagcaat aaatacagag
     4741 atgatggcaa aaccggccag gaacttgacg tttttggtca tcatgtacat gacagcaccg
     4801 accagaactt cagccagaac aacccattta acaacactgg agtccttacc gaaggtcgcc
     4861 ttaaccgtgg tgttaccgct tgccatcagg tcctgaccac tgctgccggc ggccatcgcc
     4921 agctgcgaga agaacatcat cagaacagca gccgggatca ctgcgcgagc caggagaagc
     4981 atattcagac gagtgaattt ggaaaaaaac gatttctttt tgacgggcgc agaagcaccc
     5041 tgaacactta aaacggcgtc catatgattc ctctgtttaa tattgataga taaaaactaa
     5101 agttctttaa atgttgactc gctctcttcg gtcacctccc tgaatatttc ttcgttatag
     5161 aaatcaggaa accgtttcag atgatctctg agacggatct ggacttctat cgttttactt
     5221 cttccactac gatctttggc cttaataagt cggttatttg tatcttcatc gagatacacc
     5281 gagaccgttc tgcttacacc gcctctcgcg ttacgcctcc tcactaaatt cacctctgtt
     5341 tcccgactaa taaaattatg attgcacaga aatcggcgaa aaaaaacacg ccaaaattgg
     5401 cgtgccttat ttaacattta tttaacacca taaaattcac gaattatact taatagcgaa
     5461 tctatttctc ctttatagat acatgaaact ctgaataaat caaagtgttc aaaaaactct
     5521 tttattttcc gtttaactgg tttaattcga tcctcagatg ctctgtctgt aatacccaat
     5581 tctctggcaa ttattgcgtg accaacaccc agcatataaa aagagaaaac aaccaatgtt
     5641 cctggagaaa gatcattcca tatcgttaat tgaccagaag tatttgtatt acttacactc
     5701 tgatttaacg caaaaaatgg ataatcagga aacggattaa tttgccataa aacaacagat
     5761 tcgtcattat aaaaagaaac attctccatc cttatctgat agatttcgcc atgaaaatta
     5821 aaacgacgac aaaatgcaga gccatgtcca agagcctggc attccagttc aagtgatgaa
     5881 agaaaaattt caacctgcag acgtatataa ctctctccgg ataaaggttt atcttctctg
     5941 gaaaagagtt caataaaagc cccattggca taaaggattt tccggttttt gttacggaca
     6001 caaaccggaa cgggagaatt tctgtagata ttctgaacgt taaggatgta tttatttaca
     6061 gattgactgt taagtggcct ttctctacga tccagcgcac acataggaac ctccactaag
     6121 gattctatgg acagtcgatg cagggagtta cagctccctg catcggcgat tttcagtttc
     6181 aatttttaac gtggcattaa ccacgaaatt aatatactaa ttatctgacg tattgtcatc
     6241 tttttgaatc ataaaaactc ctatatttga accaactcaa acttcatttt catttattca
     6301 tcatcatttt ttgggaaaaa ccggtccatc tcaacagaca ctttctctct gatatcgtca
     6361 accatactgg catattcaaa cttcgggttt ccggatacat gaggactgag agattcaata
     6421 cctaaaatct ttgccaccgt tgactgtgtt tttacaacac attcaagaag aagtttatta
     6481 aactctgtct ggttaaatgc agactcttta cgctccatct gagcgtcata tacacggagc
     6541 cctaattcaa gaagcattga agcagttcct gaaaggctta tatctttatc tcttgctccc
     6601 tcctgacgac gcttttcaac aatcatgtta attttttcat gaacctcatt actgatatac
     6661 agatttactc tggccataag acagtacctt ttagattctg aaataagagt tttatttcat
     6721 ctactcatag tatggtctga aaaaagaaga gcacccctag cagcgcccct agaggcatac
     6781 tataaaaaaa cgcacggcgc tgctaggggc ggccctaata tatccaaatg tttttcatga
     6841 aaattgtcag cactgaccct aacaaggg
//
LOCUS       DAJOWB010000191         6839 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJOWB010000191 DAJOWB010000000
VERSION     DAJOWB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.191"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(107..1756)
                     /gene="pgi"
                     /locus_tag="OHO85_004492"
     CDS             complement(107..1756)
                     /gene="pgi"
                     /locus_tag="OHO85_004492"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCQ2673750.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRD"
     gene            2281..3630
                     /gene="lysC"
                     /locus_tag="OHO85_004493"
     CDS             2281..3630
                     /gene="lysC"
                     /locus_tag="OHO85_004493"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCQ2673751.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAAVQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3775..4701
                     /locus_tag="OHO85_004494"
     CDS             3775..4701
                     /locus_tag="OHO85_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCQ2673752.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNHIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYNKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLADKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(4737..5528)
                     /locus_tag="OHO85_004495"
     CDS             complement(4737..5528)
                     /locus_tag="OHO85_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCQ2673753.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(5534..6661)
                     /locus_tag="OHO85_004496"
     CDS             complement(5534..6661)
                     /locus_tag="OHO85_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HCQ2673754.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
BASE COUNT         1687 a         1768 c         1632 g         1752 t
ORIGIN      
        1 tttaggatta acctcacggt atgatttccg ttaaatcaca gacaagggga tttatctgat
       61 aaaacaatgc cgacataacg tcggcattgt tattaaggtt tttaaattaa tcgcgccacg
      121 ctttatagcg gttaatcaga ccattggtcg agctatcgtg gctgctgatt tctttatcat
      181 ctttcagctc tggcagaata cggtttgcca gttgtttacc cagctccacg ccccactggt
      241 cgaaggtgaa gatgttcagg atcacgccct gagtaaagat tttgtgctca tacagcgcaa
      301 tcaacgcccc caggctgaac ggagtgattt cacgcagcag gatggagttg gtcgggcggt
      361 taccttcgaa cactttgaac ggcaccacgt agtcaagcgt tgccggatct ttaccctgat
      421 cgcgatattc ctgctcaacc acttcgcggg atttaccaaa cgccagcgct tcagtctgag
      481 caaagaagtt agacagcagt ttctggtggt gatcagagag cgggttatgg gtgatagccg
      541 gagcgatgaa atcgcacggt accattttgg ttccctggtg gatcagctgg tagaacgcgt
      601 gctgaccgtt agtgcctggt tcaccccaga taatcgggcc agtctggtaa tccacaacgt
      661 taccgttacg gtcaacatac ttaccgttgg actccatatt gccctgctgg aagtacgccg
      721 cgaaacggtg catatactgg tcatacggca gaatcgcctc agtttccgca ccaaagaaat
      781 tgttgtacca gatgccaatc agcgccagca gtacaggcag gtttttctcg gcaggcgtgg
      841 tggagaaatg cttgtccatc gcgtgtgcgc cagaaagcag ttcaacgaag ttatcaaagc
      901 cgatggagag aacaatcgac aggccaatcg ctgaccacaa agagtaacgg ccgccgaccc
      961 agtcccagaa ctcgaacatg ttggcagtat caataccaaa ctcgccaacg gctttggcat
     1021 tggttgagag cgccgcaaag tgtttcgcaa cgtgtttttc atcacctgcc gctttcagga
     1081 accagtcacg cgcgctatgg gcgttggtca tggtttcctg agtggtgaag gttttagatg
     1141 ctaccaggaa cagcgtggtt tccgggttta cttttttcag cacttccgcg atgtgagtcc
     1201 catcgacgtt agaaacaaag tgcatgttca ggtggttttt gtacggacgc agtgcttcgg
     1261 tcaccatgta cgggccgagg tcagaaccgc cgatcccgat gttcactacg tcagtgattg
     1321 ctttgccggt ataccccttc cactcaccgg agataatcgc ttccgagaag gttttcatct
     1381 tctccagcac cgcgttgact tccggcatta cgtctttgcc atcaaccaga atcggggtat
     1441 tgctacggtt acgcagcgct acgtgcagca cggcgcggtt ttcagtgcgg ttgatcttct
     1501 cgccagagaa catcgactta atcgcgcccg ccagatcgca ctctttcgcc agatcctgta
     1561 atttcgccag cgtctcttca gtgatgcggt ttttggagta atccaccagc atctgatcgt
     1621 cgaatgttgc ggagaactta gaaaaacgat cgccgtcttt agcaaaaaga tcggcgatcg
     1681 taacgtcttt catttcatcg aagtgtttct gtagtgcctg ccaggcagcg gtctgcgttg
     1741 gattgatgtt tttcattagc aatactcttc tgattttgag aattgtgact ttggaagatt
     1801 gtagcgccag tcacagaaaa atgtgatggt tttagtgccg ttagcgtaat gttgagtgta
     1861 aacccttagc gcagtgaagc atttattagc tgaactactg accgccagga gtggatgaaa
     1921 aatccgcatg accccgtcgt tgacaaccgc cccgctcacc ctttatttat aaatgtacta
     1981 cctgcgctag cgcaggccag aagaggcgcg ttgcccaagt aacggtgttg gaggagccag
     2041 tcctgtgata acacctgagg gggtgcatcg ccgaggtgat tgaacggctg gccacgttca
     2101 tcatcggcta caggggctga atcccctggg ttgtcaccag aagcgttcgc agtcgggcgt
     2161 ttcgcaagtg gtggagcact tctgggtgaa aatagtagcg aagtatcgct ctgcgcccac
     2221 ccgtcttccg ctcttccctt gtgccaaggc tgaaaatgga tcccctgaca cgaggtagtt
     2281 atgtctgaaa ttgttgtctc caaatttggc ggtaccagcg tagctgattt tgacgccatg
     2341 aaccgcagcg ctgatattgt gctttctgat gccaacgtgc gtttagttgt cctctcggct
     2401 tctgctggta tcactaatct gctggtcgct ttagctgaag gactggaacc tggcgagcga
     2461 ttcgaaaaac tcgacgctat ccgcaatatc cagtttgcca ttctggaacg tctgcgttac
     2521 ccgaacgtta tccgtgaaga gattgaacgt ctactagaga acattactgt tctggcagaa
     2581 gcggcggcgc tggcaacgtc tccggcgctg acagatgaac tggtcagcca cggtgagctg
     2641 atgtcgaccc tgctgtttgt cgaaatcctg cgcgaacgcg atgttcaggc acagtggttt
     2701 gatgtacgta aagtgatgcg taccaacgac cgatttggtc gtgcagagcc agatgtagcc
     2761 gcgctggcgg aactggccgc ggtgcagctg ctcccacgcc tcaatgaagg cttagtgatc
     2821 acccagggat ttatcggtag cgaaaataaa ggtcgtacaa caacgcttgg ccgtggaggc
     2881 agcgattata cggcagcctt gctggcggag gctttacacg catctcgtgt tgatatctgg
     2941 accgacgtcc cgggcatcta caccaccgat ccacgcgtgg tttccgcagc aaaacgcatt
     3001 gatgaaatcg cgtttgccga agcggcagag atggcaactt ttggtgcaaa agtactgcat
     3061 ccggcaacgt tgctacccgc agtacgcagc gatatcccgg tctttgtcgg ctccagcaaa
     3121 gacccacgcg caggtggtac gctggtgtgc aataaaactg aaaatccgcc gctgttccgc
     3181 gcgctggcgc ttcgtcgcaa tcagactctg ctcactttgc acagcctgaa tatgctgcat
     3241 tctcgcggtt tcctcgcgga agttttcggc atcctcgcgc ggcataatat ttcggtagac
     3301 ttaatcacca cgtcagaagt gagcgtggca ttaacccttg ataccaccgg ttcaacctcc
     3361 actggcgata cgttgctgac gcaatctctg ctgatggagc tttccgcact gtgccgggtg
     3421 gaggtggaag aaggtctggc gctggtcgcg ttgattggca atgacctgtc aaaagcctgc
     3481 ggcgttggca aagaggtatt cggcgtactg gaaccgttca acattcgcat gatttgttac
     3541 ggcgcatcca gccataacct gtgcttcctg gtgcccggcg aagatgccga gcaggtggtg
     3601 cagaagctgc attttaattt gtttgagtaa tatttcccta ctggcgtata acgctcaccg
     3661 attgttatac gccatcctca ctgaaaacat agattaaata atatctgaaa agcaaaatca
     3721 ctcctgtaga acagtattat aatcaccgaa aaatatcatt taacaggagt gttaatggct
     3781 actttaatgg aaaaagatgc acttttaaac ggagccagtc agtgcatcgc gtttttatca
     3841 aatatcattg ataattgctc agtttcgtcg catcaggatt caggcgatgc tctcaagcga
     3901 ttagtcagct atcgcgatta tctttattct acccccgctg aacttgttga ttttacacaa
     3961 ggtaagattc tccttcagca ggtaagaact caatatcagc atgaattcaa caatactacg
     4021 cattcagaaa ataaagcatc ttttgattct atttggcagc gtctgacaaa tcatgaagtc
     4081 acaccgcaac agcatcccat tggttttgtt ttaggtgggc aacccggagc aggtaaatca
     4141 tctctgattg aattagccaa aagagagaca aaagataata taatgattat taacggtgat
     4201 gattttcgat ttctacaccc tgattttaat catatctacc aaaattatgg ggatgatttt
     4261 gttacccata cagcgaaatt ttccggcgaa acggtcgaac gtgcaattga gagagcgata
     4321 gtcagtaaac tcaacattgt tgtcgaagga acatttcgta atgccgctac gccgttacaa
     4381 acattaaaaa aactcaaaga tgcaggctac caaacagaag tcatgataaa aacaacctca
     4441 gcggcattaa gctgggaaag caccaatgaa cggtataata aggacaaaga ggcgggaaat
     4501 attgcccgaa aagtggataa aaaccaccat gatatcgtca ccggtttgct ggcagaaaat
     4561 gcccgcaagg tgtttgcttc aaacctggca gataagtttg cggtgtattc gcgtgagaag
     4621 atgatattta gttcacaagc ggcaacgaat gacgatattg caacgcttat acagaatgaa
     4681 atcagcggga atacacaata ataataagag ctgatggcgt gaaataacgc cgggctttat
     4741 gcccggcgca ttaccgtctg gtacattaag ctatccagtt ccttaacgtc cccttctctg
     4801 ctatccggta acagcggtgg aatttcccca cgcgccagtt ccgcacgaat aaacgcatgt
     4861 aacaatggac ggcgcggacc atattccgct tctcctgcgc gctgtttacg ttccagtaat
     4921 tcgtctattt ctgcacgtaa cggcgcatca agttcgcttc ccgccagtaa ctcagcaaag
     4981 cgcattggcg gtacgccttt acccgcttcg acccagcgca cggccagcag cggacgcagc
     5041 acgtaaaagt attttttcag tcgaacttcg tcgccctgca aatatccggg gaagtttttc
     5101 tgcgccatcg aatagtaatg ccagcgcgca cgcagcggag aaaaccacat tgggaccatt
     5161 gccttaaacg ccgttatcgt ttcttcatct tgttgataaa ccaccggaga atccagccat
     5221 tctatcagcg tcgggttcgc gcctttcaat aatcccagcg ccttacgcca ctcccaaccg
     5281 gaaacatcta actcgtcgtc gatgggcagc tcaataacat cgcgcggtga ctctactcgc
     5341 agataccatt ccagcgggtg aacatacaaa aaccgtacat cgtagtcgct gtcgggcgag
     5401 gcaaatcccc agccgcggct ccctgattca caggcgtaca gtactttcac atcataccgc
     5461 cgttcaattt cttttaactg ctgcgatacc ctttctcgca tcgcagcact gactccattt
     5521 aatggcatgt tactcaccct ttgatattaa ccaacccatg cagaacctga tccagaccgc
     5581 caaatacaga gatgttatcg atccgttcag ccactctctc cagcgtttcc agttctttta
     5641 agcgtaacgc taccgggttg ttttccatga ctttagcggt attgagtaac gaacgcgtcg
     5701 ctgccgtttc ttcacgtcgg cgaatcacat tggcctgcgc ggatttctcc gcctcaacca
     5761 gctgcgcaag gatatttttc atatcgccag gcaggacgat atctttcacc cctaacgagg
     5821 caatctccat accaaatggc aacatgcgcg atttcacctg ttcgctgaca acgtcatcaa
     5881 tgacctgttt atcttccagc aattcatcca acgtacgcgt acctaccgct tcacgcaggg
     5941 caaattgtaa ttcccgatac aaatgatcaa tcggcttcgt taactggcta aatgccaata
     6001 acacgtcgct gtagcgccag ttagcggcga ggttaatccg cagattgacc ttatctttcg
     6061 ttaagatttc ttgcccgctg acttccagta cctgcaagcg ggtgtcaacc acttcagcgt
     6121 caaccagatg gttaattttc cagtatgcgg tcagccccgg cggtaacagc gcctgcgttt
     6181 caccatcgat tttcagcacg cctacatgcc aggccggaac ctgcaccgtt agtattgcat
     6241 cgcggccttt aactgcccct ttgcgccgtg gttgcaatac cgcattcatc acctccgttt
     6301 gtacctggac ttgctgcgta ttcatccgca ccagtgtcaa atcatcctct acacgccagt
     6361 acaaacggcg cgttgatggc ggcaggatct cctgcaaaat accgtccata tacaacgccc
     6421 cggcttcaat ctccgacaag tctgccacca ggcaatattt ctccacccaa tcaggctgaa
     6481 agcggcgcaa ataatccgcc agcacgtcgg gaacttcgct gccatcgagc gtaatcagca
     6541 agacttctgg cgtatttaac cagggtaaaa gatgctcgcc tgcatgtagc actttgtagt
     6601 aatcgccgtt tctggagagt aatgccagct gatcttttct tacgcttatt tttttaatca
     6661 ttatctattc cttgcttaac agccgtcgcg gaagcagaca cgaagcgaca ggggaattcc
     6721 cttttgcctg ttacccggca tctttatcgg cgacgttatc ggggatcgct gactgcgcaa
     6781 tccctcactt tttatttaat aagttaaaac cagtattcat ttcaggaatg aatgtggga
//
LOCUS       DAJOWB010000192         6809 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJOWB010000192 DAJOWB010000000
VERSION     DAJOWB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.192"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1956
                     /gene="acnA"
                     /locus_tag="OHO85_004497"
     CDS             <1..1956
                     /gene="acnA"
                     /locus_tag="OHO85_004497"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCQ2673755.1"
                     /translation="PVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGKF
                     VEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVQLVEK
                     YAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNELE
                     VNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVTLG
                     LKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDPIE
                     TAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAAEPIG
                     HDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDTYG
                     WQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSPAG
                     RYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDVV
                     SIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNL
                     IGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVPCR
                     CRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(2020..2610)
                     /gene="ribA"
                     /locus_tag="OHO85_004498"
     CDS             complement(2020..2610)
                     /gene="ribA"
                     /locus_tag="OHO85_004498"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCQ2673756.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            2780..3544
                     /gene="pgpB"
                     /locus_tag="OHO85_004499"
     CDS             2780..3544
                     /gene="pgpB"
                     /locus_tag="OHO85_004499"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCQ2673757.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            3693..4001
                     /gene="lapA"
                     /locus_tag="OHO85_004500"
     CDS             3693..4001
                     /gene="lapA"
                     /locus_tag="OHO85_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCQ2673758.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            4008..5177
                     /gene="lapB"
                     /locus_tag="OHO85_004501"
     CDS             4008..5177
                     /gene="lapB"
                     /locus_tag="OHO85_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCQ2673759.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            5370..6107
                     /gene="pyrF"
                     /locus_tag="OHO85_004502"
     CDS             5370..6107
                     /gene="pyrF"
                     /locus_tag="OHO85_004502"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCQ2673760.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            6107..6433
                     /gene="yciH"
                     /locus_tag="OHO85_004503"
     CDS             6107..6433
                     /gene="yciH"
                     /locus_tag="OHO85_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCQ2673761.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(6559..6777)
                     /gene="osmB"
                     /locus_tag="OHO85_004504"
     CDS             complement(6559..6777)
                     /gene="osmB"
                     /locus_tag="OHO85_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCQ2673762.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         1666 a         1612 c         1822 g         1709 t
ORIGIN      
        1 ccggtttcca tgcttatccc ggacgtagtg ggctttaaac ttaccggtaa attacgtgaa
       61 ggtattaccg ccacagacct ggttctcact gttacccaaa tgctgcgcaa acatggcgtg
      121 gtggggaaat tcgtcgaatt ttatggtgat ggtctggatt cactaccgct ggcggatcgc
      181 gccaccattg ccaatatgtc gccagaatat ggtgccacct gtggcttctt cccaatcgat
      241 gctgtaaccc tcgattacat gcgtttaagc gggcgcagcg aagatcaggt tcagttggtc
      301 gaaaaatatg ccaaagcgca gggcatgtgg cgtaacccgg gcgatgaacc gatttttacc
      361 agtacgttag aactggatat gaatgacgtt gaagcgagcc tggcagggcc aaaacgccca
      421 caggatcgcg ttgcactgcc cgatgtacca aaagcatttg ccgccagtaa cgaactggaa
      481 gtgaatgcca cgcataaaga tcgccagccg gtcgattatg ttatgaacgg acatcagtat
      541 cagttacctg atggcgctgt ggtcattgct gcgataacct cgtgcaccaa cacctctaac
      601 ccaagtgtgc tgatggccgc aggcttgctg gcgaaaaaag ccgtaactct gggcctcaag
      661 cggcaaccgt gggtcaaagc gtcgctggca ccgggttcga aagtcgtttc tgattatctg
      721 gcaaaagcga aactgacacc gtatctcgac gaactggggt ttaaccttgt gggatacggt
      781 tgtaccacct gtattggtaa ctctgggccg ctgcccgatc ctatcgaaac ggcaatcaaa
      841 aaaggcgatt taaccgtcgg tgcggtgctg tccggcaacc gtaactttga aggccgtatc
      901 catccgctgg ttaaaactaa ctggctggcc tcgccgccgc tggtggttgc ctatgcgctg
      961 gcgggaaata tgaatatcaa cctggctgct gagcctattg gtcatgatcg taaaggcgac
     1021 ccggtttatc tgaaagatat ctggccatcg gcacaagaaa ttgcccgtgc ggtagaacaa
     1081 gtctccacag aaatgttccg caaagagtac gcagaagttt ttgaaggcac agcagagtgg
     1141 aaggaaatta acgtcacacg atccgatacc tacggttggc aggaggactc aacctatatt
     1201 cgcttatcgc ctttctttga tgaaatgcag gcaacaccag caccagtgga agatattcac
     1261 ggtgcgcgga tcctcgcaat gctgggggat tcagtcacca ctgaccatat ctctccggcg
     1321 ggcagtatta agcccgacag cccagcgggt cgatatctac aaggtcgggg tgttgagcga
     1381 aaagacttta actcctacgg ttcgcggcgt ggtaaccatg aagtgatgat gcgcggcacc
     1441 ttcgccaata ttcgcatccg taatgaaatg gtgcctggcg ttgaaggggg gatgacgcgg
     1501 catttacctg acagcgacgt agtctctatt tatgatgctg cgatgcgcta taagcaggaa
     1561 caaacgccgc tggcggtgat tgccgggaaa gagtatggat caggctcaag ccgtgactgg
     1621 gcggcaaaag gtcctcgtct gcttggaatt cgtgtggtga ttgccgaatc gtttgaacga
     1681 attcaccgtt cgaatttaat tggcatgggc atcctgccgc tggaatttcc gcaaggcgta
     1741 acgcgtaaaa cgttaggact aaccggggaa gagaagattg atattggcga tctgcaaaat
     1801 ctacaacccg gtgcgacggt tccggtgacg cttacgcgcg cggatggtag ccaggaggtc
     1861 gtaccctgcc gttgtcgtat cgacaccgcg acggagttga cctactacca gaacgacggc
     1921 attttgcatt atgtcattcg taatatgttg aagtaacaag tatttgcttg ccggttattt
     1981 tgcttccggc aagcaaacta attacacaat gcaagagggt tatttgttca gcaaatggcc
     2041 cattttctcg gctttggtat cgagataatg ttcgttattg gggttacgac ctacaatcaa
     2101 tggtacgcgt tcaacaatat taatccctgc ttcggtcaga atttcgactt ttttcgggtt
     2161 attggttaac aagcggactt cattgacgcc aaggagtttg aacatatcag cgcaaagagt
     2221 gaagtcgcgc tcatcagcgg cgaagcctaa ctggtggtta gcctctacgg tatcgtaacc
     2281 ttgatcctgc agtgcgtaag cgcggatttt attcagcaga ccaatgttac gaccttcctg
     2341 acggtgatac agcaaaatac cacggccttc ctcggcaatt tgcgtcaatg ccgcttcgag
     2401 ctggaagcca caatcgcagc gcaagctgaa cagggcgtca ccggtcagac attcggaatg
     2461 gacgcgcgca agtaccgggg tatgcccgga aatatcgcca tagactagcg cgacatgatc
     2521 gtgtccggtt gccagttctt caaatcccac catcaggaaa tcgccccatg gggttggcag
     2581 tttggcttct gccacacgtt taagctgcat gaaattctcc agataatgct ggttctgtat
     2641 tggcttattt tgccataacg agaaggggtt cacctaatca cgacgcgtcg atcgttcacg
     2701 gaatggcaca aatctgtcaa tttttcctgg aactggcgtt ttcagttatg attgtgggac
     2761 ttatcaaaaa ggagaggcca tgcgttcgat tgccagacgt accgcagtgg gagctgcact
     2821 attgcttgtc atgccagtag ccgtatggat ttctggctgg cgttggcaac ctggagaaca
     2881 aagttggcta ctaaaagcgg ctttttgggt tactgaaact gtcacccagc cctggggcgt
     2941 cattacacat ttgattttat tcggctggtt tctctggtgt ctgcgttttc gcattaaggc
     3001 tgcctttgta ttatttgcca ttctggcggc cgcaatcctt gtgggacaag gcgttaaatc
     3061 ctggatcaaa gacaaagtcc aggaaccacg accttttgtt atctggctgg aaaaaacaca
     3121 tcatattccg gttgatgagt tctacacttt aaagcgagca gaacgcggaa atttagtgaa
     3181 agaacagttg gctgaagaga aaaatatccc acaatatttg cgttcacact ggcagaaaga
     3241 gacggggttt gcctttcctt ccggtcacac gatgtttgct gccagttggg cactgctggc
     3301 cgttggtttg ctgtggccgc gtcggcgaac gttaaccatt gctatcttgc tggtctgggc
     3361 aacgggagtc atgggaagcc gcctgctgct cgggatgcat tggccacgcg atctggtagt
     3421 agctacgttg atttcgtggg cgctggtggc ggtggcaacg tggcttgcgc aacgaatttg
     3481 tgggccatta acaccacctg cggaagaaaa tcgcgaaata gcgcaacgag aacaagaaag
     3541 ttaacgctgg ttgattttcc gaatttagcc cttaaatcat caacaatgcg cgtggatgcc
     3601 atttcgcaga cggcgcgaaa atggtacttt aaagggctat tgcggtaagt tgaccataat
     3661 ttattcgctc taaccacata acgggaagta atgtgaaata tttactcatt ttcttactgg
     3721 tgttagcgat cttcgtgatt tcggtcacgt tgggtgcgca gaacgatcaa caggtgacgt
     3781 ttaattatct gttagcgcaa ggggagtacc gtatttccac attgctggcg gtattgtttg
     3841 ctgcagggtt tgctatcggt tggttgattt gtggcctgtt ctggctgcga gttcgtgttt
     3901 ccctggcgcg cgctgaacgt aaaataaagc gactggaaaa ccagctttca cccgcgactg
     3961 acgtggctgt agtgccgcac tcgtcagcgg cgaaggaata actttctatg ctggagttgt
     4021 tgtttctgct tttgcctgta gccgctgcct atggctggta tatgggccgc agaagtgcgc
     4081 aacaaaacaa gcaagatgaa gccaaccgct tgtcgcgtga ttacgtagcg ggggttaact
     4141 tcctgcttag taatcaacag gataaagcgg tagacctgtt tctcgatatg cttaaagagg
     4201 atacaggcac cgttgaagcc caccttacgc tcggaaacct gttccgttcg cgtggcgaag
     4261 ttgatcgcgc tattcgcatc catcagaccc taatggaaag cgcctcgctg acctatgaac
     4321 agcgtctgtt ggcgattcaa caactagggc gtgattacat ggccgccggg ttatacgacc
     4381 gcgcggaaga catgttcaat cagctgaccg atgaaactga cttccgcatt ggcgctctgc
     4441 aacagttgct acaaatctac caggctacca gtgagtggca gaaagcaatt gatgttgccg
     4501 aacgcctggt gaagctgggt aaagataaac agcgcgtcga aattgcccat ttctactgtg
     4561 agttagccct gcagcatatg gccagcgacg atctcgatcg tgccatgacc ttgctaaaaa
     4621 aaggggcggc ggcagataaa aacagcgccc gcgtatccat catgatggga cgcgtgttta
     4681 tggcgaaagg agaatacgcc aaagccgtcg aaagtttgca acgcgtgata tcccaggaca
     4741 gagaactggt cagcgaaacg ctggaaatgt tgcaaacctg ctatcagcag ttgggtaaaa
     4801 ctgccgaatg ggcagaattc ctgcaacgcg cggtggaaga gaacaccggt gccgatgctg
     4861 aattgatgct tgctgatatc atcgaagcgc gcgacggtag tgaggccgca caggtctata
     4921 ttacgcgcca gcttcagcgt catccgacca tgcgtgtgtt ccataagtta atggattacc
     4981 acttaaatga agcggaagaa gggcgtgcca aagagagcct gatggtgctg cgtgacatgg
     5041 ttggcgaaaa ggtacgtagt aagcctcgtt atcgctgcca gaaatgtggt tttaccgcat
     5101 acaccctcta ctggcattgt ccgtcttgtc gggcctggtc aaccattaaa ccgattcgcg
     5161 gtcttgatgg cctgtaattt ttaaaaaaat cctactttag ttacaacata ctagttattg
     5221 atgttccatt gtgctccggc aacgacggcg cagaaaaagc ctgccagggg aaaaatcgca
     5281 actgttaatt ttttatttcc acgggtagaa tgctcgccgt ttacctgttt cgcgccactt
     5341 ccggtgccca tcatcaagaa ggtctggtca tgacgttaac tgcttcatct tcttcccgcg
     5401 ctgttacgaa ttctcctgtg gttgttgccc ttgattatca taatcgtgat gacgcgctgt
     5461 cctttgtcga caagatcgac ccacgcgatt gtcgtctgaa ggtcggcaaa gagatgttta
     5521 cattgtttgg gccacagttt gtgcgcgaac ttcaacagcg tggttttgat atctttcttg
     5581 acctgaaatt ccacgatatt cctaacactg ctgcgcacgc tgtcgctgct gcagctgact
     5641 taggcgtgtg gatggtgaat gttcatgcct ctggtggggc gcgtatgatg accgcagcgc
     5701 gtgaggcact ggttccgttt ggcaaagatg caccgctttt gattgctgtg acagtgttga
     5761 ccagcatgga agccagcgac ctggtcgatc ttggcatgac actgtcacct gcagattatg
     5821 cagaacgtct ggcggcactg acgcaaaaat gtggccttga tggtgtggtg tgttctgctc
     5881 aggaagctgt gcgctttaaa caggtattcg gtcaggagtt caaactggtt acgccgggca
     5941 ttcgtccgca ggggagtgaa gctggtgacc agcgccgcat tatgacgcca gaacaggcgt
     6001 tgtcggctgg tgttgattat atggtgattg gtcgcccggt aacgcaatcg gtagatccag
     6061 cgcagacgct gaaagcgatc aacgcctctt tacagcggag tgcatgatga gtgattccaa
     6121 cagccgtctg gtctactcaa cggaaaccgg acgtattgat gagcccaaag cggcccctgt
     6181 acgccctaaa ggcgacggtg tggtgcgtat tcagcgtcag accagtggac gtaaaggtaa
     6241 gggcgtttgc ctgattaccg gtgtcgatct cgatgatgcc gaactgacaa aacttgcagc
     6301 ggaactgaag aaaaaatgcg gctgcggcgg agcagttaaa gatggagtta ttgaaatcca
     6361 gggcgataag cgtgatttat taaaatcact acttgaagcg aaagggatga aggtaaaact
     6421 cgcaggcggt taacataaaa agccacggat atatccgtgg ctttcgaata ttttactgtg
     6481 cgtattattc agttatttcg tactgaacag aatcaagcgt attataattg cgagtcgata
     6541 atcgacattt atttacgatt atttaccgac ctggtgacca ataacaccac cgacagctgc
     6601 accacctaat gtacccaacg tactgccatc ggtcagtact gcaccaccta atgcccctgc
     6661 acccgcgccg attgcggtgt tgcggtcccg tttagaccag ttagaacagg cactcagaga
     6721 cattgccaaa gtaattgcca gaacagccgc ggtcattttt ttgctcgtta caaacataat
     6781 actctctcct gaatttatga ttcacggaa
//
LOCUS       DAJOWB010000193         6551 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJOWB010000193 DAJOWB010000000
VERSION     DAJOWB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.193"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..1207)
                     /gene="uidB"
                     /locus_tag="OHO85_004505"
     CDS             complement(<1..1207)
                     /gene="uidB"
                     /locus_tag="OHO85_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCQ2673763.1"
                     /translation="MLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIF
                     SVLVFWVPTDWSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAA
                     RGIAASLTFVCLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENV
                     VRIVAQPSLKISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFT
                     VLVLVQNLVGTVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALV
                     ALAIASIGQGVTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIP
                     AFILGLSGYIANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVE
                     IDNRKKMQQQ"
     gene            complement(1138..1350)
                     /locus_tag="OHO85_004506"
     CDS             complement(1138..1350)
                     /locus_tag="OHO85_004506"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673764.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTTSLASVPL
                     RRAPCCYWCGYSMPSPTSLPDEWWTV"
     gene            complement(1347..3158)
                     /gene="uidA"
                     /locus_tag="OHO85_004507"
     CDS             complement(1347..3158)
                     /gene="uidA"
                     /locus_tag="OHO85_004507"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCQ2673765.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAANKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(3547..4137)
                     /gene="uidR"
                     /locus_tag="OHO85_004508"
     CDS             complement(3547..4137)
                     /gene="uidR"
                     /locus_tag="OHO85_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCQ2673766.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(4366..5133)
                     /gene="hdhA"
                     /locus_tag="OHO85_004509"
     CDS             complement(4366..5133)
                     /gene="hdhA"
                     /locus_tag="OHO85_004509"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCQ2673767.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLYSPAASWVSGQILTVSGGGVQELN"
     gene            complement(5245..6273)
                     /gene="malI"
                     /locus_tag="OHO85_004510"
     CDS             complement(5245..6273)
                     /gene="malI"
                     /locus_tag="OHO85_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCQ2673768.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
BASE COUNT         1662 a         1739 c         1538 g         1612 t
ORIGIN      
        1 attgctgctg cattttttta cgattatcaa tttcaaccac gatttctttg aattttttat
       61 ccgtgagcgg ataaaaccag ataataacga atgccagtag cataaatccg caaggtacta
      121 aggcaattga tgtgcggatg cccataataa cttccggcgt ttgcacctga ttggcgatat
      181 atccgcttaa tcccaaaata aaggcaggaa ttgaacctcc gattgcctga ccgcatttac
      241 gggtaaatga gaatagtgaa taggtgagcc cttcaattcg cacgccggtc aggtattcac
      301 cgtattctac ggtatcagct tccagtgccc acatcacagt catagtaacg ccctgaccaa
      361 ttgaagcgat ggccaacgca accaacgcca ccggcagtga ccagacggaa acccagaaga
      421 atagcagata accgcaggtt cccagcaaag cgccaatcag gaaggtattc tttttaccga
      481 tcctcgcgac catccccggc accagcggtg ccgatgccac agtaccgacc aggttttgca
      541 ccagtaccag cacagtaaac agcccggtat catttaacac atagcgcacg tagaacaacg
      601 acgaggcgct aaccgcgaag gtcgaaatca gcacacacag cgcaccgatg cacaacataa
      661 acagcgggcg attccgtttc agggtttgca gactgatctt caatgacggc tgcgccacga
      721 tacgtaccac attctcacgc gtcgatttga agcagatgaa gtaaagcacc attccggcaa
      781 tcgccagcac aatcgtccag aaatggtata ccgacaccat ctcttccggg ctggagttct
      841 taatgctcgg tcctatcaga aatgccaggc agacaaaggt taatgaagcg gcaatcccgc
      901 gagccgcgcc cagacgggcg cgggattgtg gttgttgggt catcgcggta gcaagtgaac
      961 cataaggaat attcaccagg ctgtagcaaa gcccgaggcc catgtaggtc aaatatgcat
     1021 acaccacttt gctaccatgg ctccagtcgg tcggcaccca gaataccagc acgctgaaga
     1081 tcattaacgg cgcagtaccg aagagtaaaa acgggcggaa ttttccccag cgggtattca
     1141 cactgtccac cactcgtccg gcaaagacgt cggcgaaggc atcgaatacc cgcaccagta
     1201 acagcatggt gcccgccgca gcggcaccga cgccagcgac gtggtgtagt aactcaacag
     1261 gaagagcgcc cccattgcga aggcgaagtt attggcgacg tcaccgaggc tgtagccgac
     1321 gatggtgcgc caggagagtt gttgattcat tgcttgcctc cctgctgcgg tttttcaccg
     1381 aagttcatgc cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg
     1441 cgagtgaaga tccctttctt gttacctcca acgcgcaata tgccttgcga ggtcgcaaaa
     1501 tcggcgaaat tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga
     1561 tacatatcca gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc
     1621 agcccggcta acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc
     1681 tgccaggcca gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg
     1741 acataccaac cgtaataacg gttaaggcac agcacatcaa agagatcgct gatggtatcg
     1801 gtgtgagcgt cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta
     1861 cgcgttgctt ccgccagtgg cgcgaaatat tctcgtgcac cttgcggacg ggtatccggt
     1921 tcgttggcaa tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct
     1981 ttaatcgcct gtaagtgcgc ctgctgagtt tccccgttga ctgcctcttc gctgtacagt
     2041 tctttcggct tgttggccgc ttcgaaacca atgcctaaag agaggttaaa gccgacagca
     2101 gcagtttcat caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg
     2161 taagggtaat gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg
     2221 tgcaccatca gcacgttatc gaatcctttg ccacgcaagt cagcatcttc atgacgacca
     2281 aagccagtaa agtagaacgg tttgtggtta atcaggaact gttcgccctt cactgccact
     2341 gaccggatgc cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg
     2401 cacagttcat agagataacc ttcacccggt tgccagaggt gtggattcac cacttgcaaa
     2461 gtcccgctag tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg
     2521 ctgacatcac cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg
     2581 acatgcgtca ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg
     2641 ctgcgatgga tcccggcata attaaagaaa tcatggaagt aagactgctt tttcttgccg
     2701 ttttcgtcgg taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca
     2761 caaacggtga tacgcacact tttcccggca ataacatacg gcgtgacatc ggcttcaaac
     2821 ggtgtatagc cgccctgatg ctccatcact tcctgattat ttacccacac tttgccgtaa
     2881 tgagtgaccg catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag
     2941 acttcgcgct gataccagac gttgcccaca taattacgaa tatctgcatc ggcgaactga
     3001 tcgttaaaac tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa
     3061 cgctgatcaa ttccgcagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt
     3121 ttgatttcac gggttggggt ttctacagga cgtaacataa gggactcctc attaagataa
     3181 taatactggt caacctttaa tctgattagt taaccaattc tgtgttgcac acagttatct
     3241 gtgagaaacc catggaagag aaatggaaat aaaaagaact gacaccgcga tcaaaaaacc
     3301 aaaaactgca atacccacag cgtgatatgg atcgcattaa tctttaaaac agcgttgaca
     3361 gaagccataa aataagcaat cgaacattcg ttcgagtatc ttgagaacga taaactctca
     3421 tgacatatgg ctacggtgaa tattttgggg ggggaattct ccgatcccgg ggagattgta
     3481 ttttgtacag ggaaattaaa gtatgaaacg cgggcacagt tatcaccagc ccgcgaaatg
     3541 agaggatcag gatgcggtta agataccgcc aatcatcgcg cgtaatccct gagcaaacga
     3601 cgcttcacgc gccagatctt ccgcttcgat atcggccaga acaccgtagg tcagatccag
     3661 cagtaaacgt gaagtcattg ccgtgttgat gtctgggctt atctcgcctt tttgctgggc
     3721 atcacgcatc cgctgagcaa caaattcagt gatcgtcata tgcttatttt taagcatggc
     3781 ggcgacctgt gggttacgca tcccttccgc cataatttcg accaccagcg cccgttgccc
     3841 aaaggcttca tgggtgagag agacaataga ctcgaccata tagtcaacaa aatgaatccc
     3901 ttcaatcggt tcccggaaac gggccagcgc cctctcctgg tcctgtaaga taatcgcctg
     3961 aatcaaggct tctttggaga tgaaatggtg atagagcgtc ccgggactaa tggcgcaaga
     4021 tttacagatg gctttcatcg aggcactgtg aaatccattt tctgaaaaaa tctctctggc
     4081 agcattgagg atccgggtcc gtgtcggttg tgcttcagtc tgcatgttat ccatcattaa
     4141 accattatag ttagcgtgaa taactagttt accgcgttgt caggtgagcg cctatcattg
     4201 ctgttgaata gtgcgaaggc acactctatt cgtatgcttt tcatccagct attggttaac
     4261 caatagccgt gatttggctg ccagttgttt agcagagacg ttgatgaatt gcacacgcta
     4321 tcccggtaaa caacacgtac ggctgtttac cggtccgtta gtgtattaat tgagctcctg
     4381 taccccacca ccggagactg tgagaatttg tccgcttacc cagctggctg caggcgagta
     4441 aaggaacaga gctgcgttag caatatcttg cggttggccc agacgtctga tcggcgtgtg
     4501 ctgtaacatt ttttgttcaa tttctggtgt aataacggat ttcagggcat cggttaatat
     4561 tgcccccggc gcaatgccat ttacccgaat atttttttca cccaggtcaa acgccatatt
     4621 tctgaccaga tgactggccg cagctttaga tgatgcatag gatgtcatgt ttatattttt
     4681 attttctgcc gccatagaag tgatggtcag aataacgcca ccgccatttt tttccatttc
     4741 tggcgcaaca agttgtgaca gatggaaaaa agaaaacaca ttcagttcat aagcacggcg
     4801 aaaatctgcc attggcatat caaacggttt aggaccaccg ccaccggcgt tgttaaccag
     4861 aatatcaact ttacccagct tactgatagc aaagtctgcc agtgcagaga gttcctgttc
     4921 ggaagtaata tcacaacggc aggcaaatgc ctgaccaccc agttgttgaa tttcgtctac
     4981 aacatggtta gctgcgtcgg cgttaatatc actgaccacc acagatgcgc cagctgtcgc
     5041 gaatgtaatg gcgatttctt taccaatacc tgcacccgca cctgtgatga tggcgcattt
     5101 tccgtcgagt ctcaggttgt cagaattaaa cacataaacc tcctgtgaac ttcagtaaaa
     5161 ataaaaggca ccgcctgtgt gccacatttt ttatcattaa ccttgaggaa aggacactac
     5221 caggtatagc agataaagag aggattattt cgccgcaatg agccgggcgg gaataataag
     5281 attgcgtgaa tgcgtctctt catgggtgat tttttgcatc atgcgatccg caagtgtggt
     5341 accaagttcc cgcgctggcg tgctggccca ggtaacaggt atatcatcaa gtgtggttgg
     5401 tgtcgcatcg gtaaatgccg ccagcgaaac ctgttgctca aagtaacgat cgactccgct
     5461 ttccccgctt tgccgccctg ctttcagcaa accaaaccat gcccccatcg caatagtttc
     5521 gttatagcaa accacggcac tgatggtcgg gttatgacgt aaaagcgccg tgatagcttc
     5581 cgctgcttgc ttctggctgg aagtgcactc caacacccaa tcgctgtgaa acggcaggcc
     5641 aaattttagt agagttgcac aatagccccc cacccgttct gcacgggtta atgaggaact
     5701 ttgccctccc agccaggcga tccgctgatg cccattgcga atgagatgct ccgtcaacaa
     5761 ctgtgcagcc tgcatgttgt ccgggcgaac cgtatcaaca tcatcaagat aactggcacg
     5821 ggaagcgaaa atcaccggga tagctttttc ttctgccatc cgtcgcaggt catcgctgct
     5881 tcctgcagcc ccggctatta ccacaccatc gacaccctga ttcagtaaca gtgaaaaccg
     5941 ctgtgccagc tgctcgccgt ctttaccgcc gtgaagcaaa aaaaccatcc gtccctgcgc
     6001 ttccagagct tccgtcaatc cggccgtcaa ttcggcgtaa aacggcgcag ataaatcacg
     6061 gacgatcaaa ccaatgacgc cgctttgtcc gccgcgcagc gccgacgcct ggcgattgcg
     6121 cacaaatccc agctcttcaa tggcggcgtt aacgcgttct cctgtggcgg tagagattcg
     6181 ccctttgcca ctaagcacca gcgaaacggt acttaccgac acgcccgcag ccagcgcaac
     6241 atcatgaatt gttatttttt tggcggtagc catgcagatg acctactccc tgattatgtg
     6301 acagataaaa cgttttacct tttatttatc ttatacccgc tattatcgtt gcgtaatgtg
     6361 atttatgcct cactaaaatt tgataaaacg ttttatcttc tcgcgcaatt tactgaatcc
     6421 agattgttct ctacgaggag tcgttttatg acggcgaaaa cagcaccgaa agtcacgctg
     6481 tgggagttct tccagcagtt aggcaaaacc ttcatgttac ccgtggcatt attgtcgttc
     6541 tgcggcatta t
//
LOCUS       DAJOWB010000194         6506 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJOWB010000194 DAJOWB010000000
VERSION     DAJOWB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.194"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            202..492
                     /locus_tag="OHO85_004511"
     CDS             202..492
                     /locus_tag="OHO85_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673769.1"
                     /translation="MTHTVTRLLPEAFERLAAIKTKYPDMKHTALVGELLKEAVALTP
                     FDNVKGVAEGSKAIRITTDQLELLKAQSAVVGCSMIQVLSAKVHQLADKLGV"
     gene            1099..1422
                     /locus_tag="OHO85_004512"
     CDS             1099..1422
                     /locus_tag="OHO85_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673770.1"
                     /translation="MNNFPLQIFVDSDTAMMVQSFIDAGISIDFDRLLRLMADNSETI
                     EDFIQSVEFNEPRMMLPIKDSNMKRLVIEQTNKYSISPERYLKAAIAILYADNILVTD
                     SKVVH"
     gene            2007..2711
                     /locus_tag="OHO85_004513"
     CDS             2007..2711
                     /locus_tag="OHO85_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673771.1"
                     /translation="MSIRDEHEMIIENKPSALDIHELSLRESFDRAWRESHEVNKGPY
                     IPPEPLEIPRVEIDFSMNEECELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDE
                     IDEMAILMELQTNSYAYFSRLDRDGWGYDPVEIGKVIADLPEGQRVIRTERAVRAPHQ
                     LSLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKLISDHQALAKEYRQELAQII
                     SFNDFLSVPREFSKAV"
     gene            2734..2949
                     /locus_tag="OHO85_004514"
     CDS             2734..2949
                     /locus_tag="OHO85_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673772.1"
                     /translation="MIDLTERYETIKSVCENLKLQTKPALRIKTKHQIITSRKPKTRK
                     IPKWCIDRIPSDAQIIGETELHYLVRH"
     gene            3074..5197
                     /locus_tag="OHO85_004515"
     CDS             3074..5197
                     /locus_tag="OHO85_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCQ2673773.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKSEKQTMQVASRIEKRLNK
                     AFKVNPTPLNDSLKVMERNVDKTVSKIEQRLKNTRVFKIKTEIEDTLKPLRQPRQPRI
                     SGNRAITAAYSANMSKLKGFDPILQKYIKSQFYGLSAKAGSMDNSKFNEKLAQLNASV
                     REAIAKARGHTSTSINGDRAANSLDVLATSAIKAGTAIYSFQAVLASYQKVMEVGLQR
                     ASAERSIDFVFDKDANQVKEFTKSLAQTTGLDIAELQSQFAGFGASAKESMGIQGSEE
                     LFRNMIGYSRLMGRSEEEIKRALTALSQMAGKGQVMAEELKGQLAEAVPGMVQVFAKA
                     TGKTEQELFDAMKKGALKSADTLQKVTQELNKQITAKGGWKTISESTQAQLGNLKNSW
                     NTTLDSIFRGSENGLQDFTRSLTTLLNALGGSGKSLGESLGSLMSDMSHGVDSLTDIS
                     YRVRAFFDEVTLAYRSLNDTQKKIVDGLSDGLIDGLKMLAVAVTAQKAANVAGGLLNL
                     ANAVTSFGDAVNKAKAGKAGSSGGALSKVGRVILYAELVNFALDNLNPEAAKNPNLHQ
                     SGIGTETNFNPEYKGDSSKPNQINEGWAKIGSSIADWFNGAANKIANFQEPMSNLVNN
                     TQSTPPAITGDMVNSNAQLAMTISELSRKVDALTVEAKKPLSVNITGEATVRPDEPAF
                     FNFSQSANDQFTQDMLLSSSYPEEE"
     gene            5361..6134
                     /locus_tag="OHO85_004516"
     CDS             5361..6134
                     /locus_tag="OHO85_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673774.1"
                     /translation="MIYTNQLRYTGKDGVYFHLQGQPDNFLSLSATENMEFDSPMMVT
                     TQNMQSGQTVTDNVMRSPRTITISGVVVVGESGSILISRDNKLVENFIDTLENWRDQK
                     QIISVVCKDGIKIDDSIITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDISSATG
                     KTATTNDGGATSKKNTGNTTTSLGNGMLMCQELYNRSTSELTNEQLQARINCGKSVKV
                     ENGKSTFSDAANEQASKVLNNAGTGSTLKKFSVNPNKRF"
     gene            complement(6149..>6506)
                     /locus_tag="OHO85_004517"
     CDS             complement(6149..>6506)
                     /locus_tag="OHO85_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCQ2673775.1"
                     /translation="EDLSAITSKLKQLKNELSQVDADIEKETQRLKSIDVGCLSDCDL
                     TTKAGRIEAQLIIKSAVKEVVLNTAKRRCKVTFHNGKVIDLSITENPSEDVTEAIQSL
                     SEVTERGLIDVDEVII"
BASE COUNT         2126 a         1168 c         1369 g         1843 t
ORIGIN      
        1 aataccgcaa agcccaacga gctaaaacga tgagattcaa taattgctgt gaaaaaatca
       61 tgcattaatt ttccttttgt gaaaggaaac agcacctgta tcagccccta taaccatatt
      121 aacatgtaaa tgagatgttg acaatatgtt aaataaatgt taagattgta gtatgagtaa
      181 gagatttaag gagattttac aatgactcac acagttacgc gccttttgcc agaggcattt
      241 gaacgactgg cagcaatcaa aaccaaatat cccgatatga agcacacagc gcttgttgga
      301 gagcttctaa aagaggcggt agcccttacc cctttcgata acgttaaggg cgttgcagag
      361 ggttctaagg ccatcagaat cacaacggac caactggaac tactcaaagc acagtccgca
      421 gtagtaggtt gctcaatgat tcaggtgtta agcgcaaagg tccatcaatt agcggataaa
      481 ttgggggttt aatgtgttat aggtacaaat tttataaact ggcatcacat ctaatcaggc
      541 aagatacccg ctcaaaagaa tatgaagatg ttacgcttga ccgtaacaag aaaagactaa
      601 agccacatca aattgagcag ttacgctatg aattaaagtc actggatatc gagcttgatt
      661 atagtgaccc gactcaattt aaacgggcct gtgaactcat taagaattgg gggaaagaat
      721 gaagaaagta ttcaccctca aacttaaaac agacaaagcc tttaaatatt ttcgaaatct
      781 aattgatgta cataatggat ggggtgacat tgataatgat ggcatctatc tgattatgca
      841 atccccatct ttcacactaa aaacctcagt aacaaaaagc tggtttagtc aatttcatag
      901 tgagatgggg ttaatagtct cagactaaag gcaaaaaacg ggtttaccca ctctcagacg
      961 ctctcaatcg cattctaacg caaaaaacta tcttatttta gggtatgtgg tacaccccta
     1021 caggctaccc ctgaaaaaga ctgtttagct cgttacagag cgattgagga ggtctggtga
     1081 aaaaggagat tttaagaaat gaacaatttc ccgctacaga ttttcgttga tagcgacacc
     1141 gctatgatgg tgcaaagctt cattgatgca ggtattagca ttgactttga caggctgtta
     1201 aggctaatgg ctgataactc agaaacaatt gaagatttta ttcagtcggt ggagtttaac
     1261 gagcctcgaa tgatgctacc aattaaagac agcaacatga aaagattggt tatcgagcaa
     1321 acgaacaaat acagcattag ccctgaaaga tatctcaagg cagctatagc aattttgtac
     1381 gctgacaata ttttggtgac ggattcgaag gtggtgcatt aatgggctta ataattgctt
     1441 ggacagctct gggtttattt gttggttgct gggtaggtaa cacagtaaat aagaattgat
     1501 gttaccaatc tgttaaaaag ctgttgacaa ataaccttga gcatgacata attgtatata
     1561 tgaggatagt cttctatccg aagtctatag ggagagcaat tctccttgaa ttaaaatatt
     1621 aatagtctct acatcgatga taactatatt ctgagttgga aaggtgcaat gcttttcgtc
     1681 atggcctttc agtttagtct gagaggtcat ctcaaaaagt gcttaatcat tcattattga
     1741 acccttacag ctttgcccta actcatgatt agggcgtttt ctttaagagt ttttaaacag
     1801 agcttttaaa gaaagttttt cctgaaactt cgttaacgtt aaagcaaacc ttcaaagccc
     1861 ttcacagatt atgtttaggg cttttttcag cttttaaaaa gcatacttgg caaaccgcct
     1921 tttaagtctc ttctaagttt gtatggctgt caggtgtgct ttttaaaaac tgaaacataa
     1981 attatcttag aagaggaaaa ataataatgt ctatccgtga tgaacatgaa atgattatcg
     2041 aaaacaaacc aagtgcactt gatattcacg aattaagttt gcgtgaatct tttgatagag
     2101 cttggcggga atcacacgaa gtaaataaag gtccttatat tccaccagaa ccactcgaga
     2161 ttccaagagt tgaaattgat ttttcgatga atgaggaatg tgagcttgat ttgaaactga
     2221 agctgcaacg ccgatatttc caagctatta aagactctca agaagcaatg agaagtgttt
     2281 acgagaaaat tcagtccaag cttgaagacg aaatcgacga aatggctatt ttgatggagc
     2341 ttcaaaccaa ttcttatgct tatttctctc gactagaccg tgacggttgg gggtatgacc
     2401 ctgtagagat tggtaaagtg attgcagatt taccagaagg tcaaagggtt attcgtacgg
     2461 aaagagctgt cagagcgcca catcaactgt ctctaattgt tgctgataaa tcacaagctg
     2521 agcttgaaga aattgccaaa gagaaactga tgaaagcaag agaaaatgag attaatttgc
     2581 tgaaatcaag actggctaag ctgattagtg accatcaagc gctggctaag gagtaccgtc
     2641 aagagttggc acagattatc agcttcaatg attttctgag tgttccacga gaatttagca
     2701 aggctgttta attatgcata tgtgaggtga tgaatgattg atttaacaga acgctatgaa
     2761 actattaagt ctgtttgtga aaacttaaag ttacaaacta agccagcctt aagaattaaa
     2821 accaaacatc aaattatcac ctcacgcaaa ccaaaaacac gcaagattcc aaagtggtgt
     2881 atcgaccgta tcccttccga tgctcaaatt ataggtgaaa cggaactaca ttatcttgtc
     2941 aggcattaaa aaagattggt tgggtgtatg agataggttc tatcgaatct ataacacggg
     3001 gagagattat cgatattatt cggtagtttc tccctttttt gtatatgaac acttaagagg
     3061 tgaaaaaata tgaatggcac aatttaaagt tgatgatttt ctgattgagt taagttttaa
     3121 ctctcagaaa gttttaaaag gcttggaaaa atccgagaaa caaacgatgc aggtagcatc
     3181 acgaattgag aagcgattga ataaagcttt taaagttaac cctacgcctt taaatgattc
     3241 tctcaaggtc atggaaagaa atgtcgataa gactgtttcc aagattgagc agcgtcttaa
     3301 aaacactaga gtgtttaaga ttaaaacgga aattgaggat actttaaagc cacttagaca
     3361 gccaagacag ccacgaatta gcggcaacag agcaatcaca gcggcttact cagcgaatat
     3421 gagcaagtta aagggctttg accctatttt gcagaaatac atcaaatctc agttttacgg
     3481 gctatccgct aaagctgggt caatggataa ttcaaaattc aatgagaagt tagctcaatt
     3541 aaatgcttca gtcagagaag caattgctaa agccagaggt catacatcca ccagcattaa
     3601 cggtgacaga gcagctaata gtctggatgt attagctaca agtgcaatca aagcaggtac
     3661 agccatttat agttttcagg ctgtactagc cagctatcag aaggttatgg aagttggcct
     3721 tcaacgtgca agtgctgaac gttcaattga tttcgtattt gataaagatg caaatcaagt
     3781 aaaagagttc acaaagtcat tagctcaaac aacgggttta gatatcgctg aactgcaatc
     3841 acagtttgct ggtttcggtg cttcagctaa agagtctatg ggtattcaag gttctgaaga
     3901 gctttttaga aatatgattg gctattctcg tttgatggga agaagtgaag aagaaattaa
     3961 gcgagcatta acagcattat cacagatggc tggtaagggt caggttatgg ctgaagagct
     4021 taaggggcaa ctagctgaag ctgttcctgg tatggttcag gtattcgcta aagcaaccgg
     4081 aaagactgag caggaactat ttgatgccat gaagaagggg gctttaaaat ctgccgatac
     4141 tcttcagaag gtcactcagg aactgaataa acaaatcaca gctaaaggtg gctggaaaac
     4201 tatttcagaa agtactcagg cgcagctagg gaatctgaaa aactcttgga ataccacgct
     4261 tgatagtatc ttcagaggtt ctgaaaatgg tcttcaagat ttcacaagaa gcctaacaac
     4321 gcttttaaac gctcttggtg gaagcggtaa aagtcttggt gaatctcttg gtagcttgat
     4381 gagcgatatg tcgcatggtg tcgatagtct gactgatatt agctacaggg taagagcgtt
     4441 cttcgatgag gtgacattag cttaccgttc actaaatgac actcagaaaa aaattgttga
     4501 tggtttatct gatgggttaa ttgatggttt gaaaatgctt gccgtagctg ttaccgctca
     4561 aaaggctgca aatgtagctg gaggcttgtt aaaccttgca aatgctgtta cttcattcgg
     4621 tgacgctgtt aataaagcaa aagctggcaa ggctggttct tctggtggtg cgctaagtaa
     4681 agtcggtaga gttatccttt atgctgagtt ggtaaacttt gctcttgata atttaaaccc
     4741 tgaagctgct aagaatccga acttacatca atcaggtatt ggcacagaaa caaacttcaa
     4801 ccctgaatac aaaggcgatt catctaaacc aaaccaaatt aatgagggat gggctaagat
     4861 tggtagttct atcgctgact ggtttaatgg tgcagctaat aaaattgcta acttccaaga
     4921 gccaatgagt aaccttgtta ataacacaca atcaactcca ccagctatta caggtgacat
     4981 ggttaatagc aatgctcaac ttgcaatgac tattagtgaa ctatcacgca aggttgatgc
     5041 tttaacagta gaagctaaga agcccttgtc agttaatatc actggtgaag caactgttag
     5101 accggatgaa ccagctttct ttaatttcag tcaatcggct aatgaccagt tcacgcagga
     5161 tatgctctta agttcaagct atccagagga agaatgattt cttatatatc cgtgtgctac
     5221 gtgaaatctg taaagctaat acccgcactg aaaaatagaa tttacgtagc tcacgcttaa
     5281 cagagaaaat acatatattt caaaggcttg gttaaaaatg attaaaatac actaagcctt
     5341 tgaaatcaaa ggagaattta atgatttaca ctaatcagct tcgttacact ggcaaagatg
     5401 gtgtttactt tcatctacaa ggccaaccag ataatttctt gtcactatca gctactgaga
     5461 atatggaatt tgatagccct atgatggtga ccactcaaaa tatgcaatca gggcagactg
     5521 taacagataa tgtaatgcgt tcacccagaa caattactat tagtggtgtg gttgtggttg
     5581 gtgaatccgg tagtatcctc atctcaagag ataataagct ggtggaaaac ttcatagaca
     5641 ctcttgaaaa ctggcgtgac cagaagcaaa ttatctcagt tgtctgtaaa gatgggatta
     5701 aaattgatga ttccattatt acaagcttta aagcctctaa agatgttggt atttcaaatg
     5761 gcctaagaat ccagctaact tttcaggaaa ttaacttcaa agctattgtg ggtcagactg
     5821 atatttcgtc tgcgactgga aagactgcta ccacgaatga tggtggtgct accagtaaga
     5881 agaacactgg caacactaca acaagcttgg gtaacggtat gttgatgtgt caggagctat
     5941 ataatcgctc tacgagtgaa cttactaatg agcaacttca agcccgtatc aattgtggta
     6001 aaagtgtcaa agttgagaat ggaaaaagca ccttctcaga tgccgctaat gagcaagctt
     6061 caaaggtgct taataatgct ggtacaggta gtactttgaa gaagtttagt gtgaatccta
     6121 acaagcgatt ctgacgatag aatctcaact atatgataac ctcatcaaca tctattaaac
     6181 ccctctcagt gacttcagaa agcgactgta tagcctctgt aacgtcttct gaggggtttt
     6241 ctgttattga taggtcaatt accttaccgt tgtgaaaagt tactttgcag cgacgtttag
     6301 cggtatttaa aactacctct ttaacagcac ttttgattat cagttgtgct tcaatccttc
     6361 ccgcctttgt agtcaagtca caatcagaca aacaacccac atcaatagat ttaaggcgtt
     6421 gtgtctcctt ctcgatatcc gcatcaacct gtgataactc attctttagt tgttttagtt
     6481 tgctggtaat agcggacaag tcttca
//
LOCUS       DAJOWB010000195         6365 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJOWB010000195 DAJOWB010000000
VERSION     DAJOWB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.195"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..396
                     /locus_tag="OHO85_004518"
     CDS             <1..396
                     /locus_tag="OHO85_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HCQ2673776.1"
                     /translation="DTQNRLSVNTLMVNTYYDFRNSSAFTPWVSVGLGYARIHHKATY
                     TDTSWNKSGEVSDISALHYSGYDNNFAWSIGAGVRYDITPDIALDLSYRYLDAGKSSL
                     SYKDAEGDKYKSEADVKSHDIMFGVTYNF"
     gene            571..2070
                     /locus_tag="OHO85_004519"
     CDS             571..2070
                     /locus_tag="OHO85_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCQ2673777.1"
                     /translation="MNIRKLFCPGNTPRILLFLFFFVVSVITTIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTTDEAAEF
                     TGMIPVYHFLVSAAIQVFMVIFWRTHRRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     TTDTLREMQRYKQLNQHGADNWKILPGVPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFL
                     HMMGSHPNPCDRLHSWPNHYLEQYPRKVACYLASISKLDNFLGQLDGILRRHSRHFAM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKISARHFAGIFQ
                     WLTGIRTENIPPFNPLTDEDNEPVMVFNGERNVPADSLKPQPLILPDRR"
     gene            complement(2179..5694)
                     /locus_tag="OHO85_004520"
     CDS             complement(2179..5694)
                     /locus_tag="OHO85_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCQ2673778.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVVLRPKVYFRLLQHGKDRSAGAPDIVTPLVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTIHTSFSINFDDSIDKTAETD
                     EEREARYAALQQEWRQYLDDSERLLKNVAGDWIKNPEQYELAEHGYIVKTAQSGGASF
                     HILSLYDHLLVCKKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGTGAMAGRWLPELKSFGAYFPSSTRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPEKECSSPEKVIELLHGQLVAKSEQLKRLNATWQTLSQ
                     VRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRSKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMARIDDLQEEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCFDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGEQASSGSRYNLLE
                     AETIAAWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQALGKQGISTGANEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTSETKIYTLHGVEQHDNFLNQTF
                     ENTDKHITIVSPWLTWQKLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHKDFEKRKE
                     KQQNLKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(6146..>6365)
                     /locus_tag="OHO85_004521"
     CDS             complement(6146..>6365)
                     /locus_tag="OHO85_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCQ2673779.1"
                     /translation="SALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
BASE COUNT         1630 a         1569 c         1375 g         1791 t
ORIGIN      
        1 gacacacaga acaggctcag tgtgaatacc ctgatggtga atacgtatta tgacttcaga
       61 aacagcagtg cattcactcc atgggtgtct gttggactgg gttatgcacg gatacatcat
      121 aaggcaacat atactgatac ctcctggaat aaatctggtg aggtgagcga tatttctgcg
      181 ttgcattact cgggatatga taacaatttc gcatggagca ttggggccgg tgttcgctat
      241 gacataaccc cggatatcgc tcttgacctc agctatcgct atctggatgc tggtaaatcc
      301 tccctgtctt acaaggatgc agaaggggat aaatataaat cagaggctga cgttaaaagt
      361 cacgacatca tgtttggtgt gacttataac ttctgacgac actgctcctg agcgataatt
      421 acacatattc agtaattaag atatttatat atcttctgca actaaacaga aataccctgc
      481 agtaatatac cgcagggtta tttgtttcac aggagattta tggtttttat tatcttattc
      541 cctttataat aatattaccg atatattccg atgaatatca gaaaactgtt ttgtccgggc
      601 aatacacccc ggattttatt gtttttattc ttttttgttg tttctgtcat aaccacaatt
      661 gcatgcggat acactgagaa gaatgccacc gggaatgtgt tgctgctgtt ccttcttctg
      721 ctccttgcac acagaaatac cctcacatcc attacagcgc tgttatttct gttctgttgt
      781 gcactgtatg cgcctgccgg tatgacgtac ggtaaaatca acaacagttt tattgtcgcg
      841 ttgttgcaga ccacaactga tgaggcagcg gagtttaccg ggatgattcc tgtttatcat
      901 tttctggtca gtgccgcgat tcaggtattc atggtgattt tctggcgaac acaccgccgt
      961 ggtcaccgta actggctggc actgctgctg ttcgtattat gctcagtaaa cagctggccg
     1021 ttgcggatgg ttaaaggaac tgttgtgggg acaactgaca cattgcgtga aatgcagcgt
     1081 tataaacaac tgaatcagca cggggctgac aactggaaaa tcctgccggg tgtaccgttg
     1141 tatgacacga ttgttatcgt tactggtgag agtgtgcgca gggattatat gtcggtatat
     1201 ggctatcccg tgccaaccac gccgtggctg aatacagctc ccggtttatt tattgacggc
     1261 tatacatcgg cagcagccag taccgtacct tccctgagcc ggacactgat ttatgactat
     1321 gagcagaacc ctgactccgg caacaacgtg gtggcgctgg cagcaaaagc aggatacagc
     1381 acatggtgga tatccaatca gggaaaactg ggggagcatg atacacgcat ctctgttatt
     1441 gcttctgatg cggagcatac cgttttcctc aagaaaggca gctttgcttc ccgtaaaacg
     1501 gatgacatgt tgttgttaca ggaaacagaa cgtgcactgg cggataaatc ctctccgaag
     1561 gtgatattcc tgcacatgat ggggtctcat ccgaacccgt gtgaccggct tcactcctgg
     1621 ccgaatcatt acctggagca gtatccccga aaggttgcct gttacctcgc cagcatcagt
     1681 aaactggata actttctcgg tcagcttgat ggtatccttc gccggcattc ccgtcatttc
     1741 gccatgcttt acttttctga ccatggtctg tcggtcagcg acagcgctaa tcctgttcat
     1801 catgatggtc atgtgcaggg aggctacagc gttcccctga ttattaccgc cagtgacatc
     1861 acgtctcatc agtccgtcag cagaaaaatc agtgcccgtc atttcgcagg tatttttcag
     1921 tggctgaccg gcattcgtac tgaaaatata ccgccattca atccgctgac agacgaagat
     1981 aatgaacccg ttatggtttt taacggggag aggaatgtac cggcagacag tctgaaaccg
     2041 cagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
     2101 ccgtgcaata atagccattc cattcagcca tcttacgctc gaaaacaatc gataagatgg
     2161 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
     2221 ctcacccttc aggttatcac cgcaataaac cattgaggta tcgtatcgtt catatcgcgc
     2281 ttcccgtgtc gcactaaacc agttgaacga tcccacgcat agcaaaccat catcaccaat
     2341 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
     2401 ctccagcgcc gctttaaggt tctgctgctt ctcttttcgc ttctcaaaat ctttatgttc
     2461 agtgttgtag cttctgtcag tgactatcgt gacgttaata ccacgtgaac acgccgcaat
     2521 catagaatca agaaaaccgg tttgctccag cttttgccag gtcagccatg gagaaactat
     2581 cgtgatgtgt ttatcggtat tttcgaacgt ctgattcagg aagttatcat gctgctccac
     2641 accatgaagt gtgtagattt tggtctcgga agtttttaaa tccttacgct ctttataatc
     2701 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggaga
     2761 tgaggctggc tggacctcaa acagatccat atcgccgaag accaggaagc tatccttcgc
     2821 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
     2881 ttttgaatag actggcgaga atataacaat cgctctttcc gctccctgaa gagagtgcac
     2941 ggtgcccact gtgagcgact tttcattcgc gcccgtactg ataccttgtt tacccagcgc
     3001 ctgtttgata gtggatacct gcgcgctaaa aggcgtcacg ataccgacaa cttcatgaag
     3061 cgatttgccg taatgcgctt caatattttg ctggttctct gccaaccagg ccgctatcgt
     3121 ttcagcctca agcaaattat atctacttcc actacttgcc tgctctcctt taccatcaat
     3181 atggagataa cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     3241 cttaccgtga tagcagagcg tattacagta tccaataata ttatcgaagc accggcggtg
     3301 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     3361 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     3421 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcaa gcatgttacc
     3481 gacatcaatc gcgggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     3541 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     3601 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     3661 tcctttgtgc tcacttatct gcatattgcc gggcagcata tagcatgtca tcaccacaca
     3721 tggagtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcgc cttttttctt
     3781 cttctcttcc tgcagatcat caattctggc catatccatc agccaacgac cttcccagta
     3841 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     3901 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     3961 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     4021 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     4081 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     4141 tatttggtac tgtcgcttac tacgaaccgc tgggagccag gaaaataatg aatagatcag
     4201 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     4261 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     4321 aataagctca cgcgcagccc gtacctggct taacgtttgc catgttgcgt tcagtctttt
     4381 caattgctcg gattttgcta ccaactgacc atgcaggagt tcaatgacct tttcagggga
     4441 tgaacactct ttttcaggaa aagctgcctt agctttctcg agataaaaca gcagtgcatc
     4501 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     4561 tgcctcagct ttacgagtgc ttgagggaaa ataagcgccg aagcttttca gctcaggtaa
     4621 ccatcgtccg gccattgcac cagtgccctg tgaaaaatct ttcccgaacg cttcgataat
     4681 gttcgttaca gcctggttgt tcgttgaagt cgcgataata accggaggct cagatttttc
     4741 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     4801 tcccggaggt ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     4861 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     4921 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     4981 attgaagagc ggcacatcct tcttgcaaac aagcaggtga tcataaagcg aaaggatatg
     5041 gaaactggca ccaccagatt gcgccgtttt aacaatataa ccgtgctcag cgagttcata
     5101 ttgctcagga tttttaatcc agtcgccggc aacgttcttc agcagcctct ctgaatcatc
     5161 cagatattga cgccactcct gctgcaaggc tgcatatcgt gcttcccgtt cttcatccgt
     5221 ttcggcagtc ttatcaatgc tgtcatcaaa gttgatagag aacgaggtat ggattgtctt
     5281 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     5341 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     5401 ggctggcgtc actagcgggg taacaatatc aggtgcgcct gcggaacggt ccttaccatg
     5461 ctgcagtaac cggaaataaa cttttgggcg caagacgaca tcgaccgttt cgacatcgtc
     5521 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     5581 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     5641 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     5701 tcccatgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     5761 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     5821 ggttgtacga ggttcagaac acaagctcat taaacagtac gtcaggactg ttgatttcat
     5881 aacagtgaat acatttttta ccgtttataa taattgcctg tgttggacgg ggataccaga
     5941 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     6001 aatcacagtc ataaatagcc tgaagaacat gacgaaggga ctttaatgga cgccggagta
     6061 acttgccaga acatcaatct tcgtcatcat ttacaaagaa aaataatcag ttaaagcagt
     6121 ccgggctgat tgcccggact gcttactatt ttaatggatt gttaattttt gcatattcaa
     6181 acggactgat acccttctcc cggttcgcat ccagaaaatc ggcccaccat tgcaacatca
     6241 gtcgccgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca ccattacgct
     6301 cctgatggct catctgacgt tccacagcat cgcgggacca caaacctgat tcaatcaatg
     6361 cactg
//
LOCUS       DAJOWB010000196         6357 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJOWB010000196 DAJOWB010000000
VERSION     DAJOWB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.196"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            180..1367
                     /locus_tag="OHO85_004522"
     CDS             180..1367
                     /locus_tag="OHO85_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000928803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCQ2673780.1"
                     /translation="MLMKYISHRVIICTLTLLIIIITVLFTFLRSSDVPEYITAPVRK
                     GDIENSVLATGRIDAIERVNVGAQVSGQLKSLKVKQGDHVTKGQLIAEIDDLPQCNDL
                     RNAEAALNEVKAELQSKQALLKQAELRFKRQLRMLRENASSHEDFESAEAMLATTRAE
                     LHSLNAKLVQAQIEVDKKKLALEYTRVVAPMDGIVIAIVTQQGQTVNSNQSAPTIIKL
                     ARLDVMTIKAQISEADITRISVGQKARFSIFSEPDKHYSATLRAVELAPESVMKDDSL
                     ASNSSASGSGTSNASVYYNALFDVPNPENRLRIAMTAQVTLITDEAQNTLLVPIQAVH
                     RNEEKKQQVLVLAADGRLEPRNVKTGITNSVDIQILEGLNVGENVVLSLPDKKEPEER
                     IML"
     gene            1364..3304
                     /locus_tag="OHO85_004523"
     CDS             1364..3304
                     /locus_tag="OHO85_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017693757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HCQ2673781.1"
                     /translation="MKKLIELKGVSRTYGNGDQTRTVLKNVDLTIVAGEMVAIIGASG
                     SGKSTLMNIMGCLDVPNRGDYYIDGQNAACLSPDELARVRREHIGFIFQRYHLIPDLS
                     ALGNVEIPAIYANSERDSRRQRATALLGRLGLEGREHHKPCELSGGQQQRVSIARALI
                     NGGKIILADEPTGALDSQSGQEVLAILNELNRRGHTVVMVTHDMKVARHAKRIIELCD
                     GEIIADSGGCVSATETLPKTNRIRQSYWKTLLDRTRESMQMALKAMKTHRLRTTLTMI
                     GIVFGIASVVTVVALGEGARQETLEEIKSLGTNVVSIYPGQDLFDDSIESIRTLVPAD
                     ANALAKQGFIDSVSPEVSASDNIRFLGKSAIASINGVGREHFRVKGIELLQGSTFRDD
                     RNALQEVIIDENTRKAIFDNAGLQALGQIVFLGSVPARIVGIAKSNNRSDASNRITVW
                     MPYSTVMYRIVGKPVLTGISVRLKDNVDNEAAISAISQLLTRRHGVKDFQLYNFEQIR
                     KSIEHTSMTFSILILMVACISLMIGSIGVMNIMLISVTERTHEIGVRMAVGARRSDIM
                     QQFIIEAVLVCLIGGALGIALSYITGALFNALAGGIFAAIYSWQAAVAAFFCSTLIGI
                     IFGYLPARKAARMDPVISLASE"
     gene            3308..4678
                     /locus_tag="OHO85_004524"
     CDS             3308..4678
                     /locus_tag="OHO85_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HCQ2673782.1"
                     /translation="MKILSSLTLCLITALCSGCTNVLKSDYRAPEVNYPINWTKGDVD
                     GNTSPFAWEEFNDPNLDNWLHLVMTSNNDIAIAALRIHRAQLDAERTGITNTPALKGA
                     LSMDGKKQLNNSSGWAKSGSASLGTSYELDLWGKIARQRDVAEWAVHASEEDFRSARL
                     MLLSEASNNYWRIGFVNQQIATLQQSIDYAKETLRLAEVRYRAGNISSLDVIDAQQNL
                     LTQENQLTGLQREHLQLLNQQAVLLGTVPGCQIVEPTTLPKGSLPKVNANIPASILMR
                     RPDISAKEWQLREALATVDIKRSEYYPTFNLTGALGTSSASLLALLHNPIGSVGANLT
                     LPFLEWRQRDIEVKIARNDYEQRVLEFKQLLYKAMSSIEDALSFRNQLLLQETRLREE
                     LELARKSEWLNEVRYRHGAVRISFWLDAQERRRQAELRLDENRFNQLQNLAKIYLEFG
                     GASTFP"
     gene            5009..6321
                     /locus_tag="OHO85_004525"
                     /pseudo
     CDS             5009..6321
                     /locus_tag="OHO85_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         1752 a         1485 c         1604 g         1516 t
ORIGIN      
        1 ctttcccggt aattagtaat actgttgaaa caaagcgaga aggggttagc ccaatttacg
       61 caaataaata ataatcttga ttatgattaa ttccaaataa gtatattttt ccagtagatg
      121 ctgggcatta actaagataa cccagaacac cactctcatt gttatggaaa acatggacaa
      181 tgttaatgaa atatatttct caccgcgtca tcatttgtac cttgacactg ttgatcatta
      241 ttatcaccgt tttgttcacc tttctgagaa gctctgatgt gccagaatat attactgccc
      301 cggtgcgcaa aggcgatata gaaaattctg ttctggcgac tggccgtatt gatgcaattg
      361 aacgggtaaa cgtaggggca caggtatcag ggcaattaaa atcactgaaa gtaaaacagg
      421 gcgaccatgt gactaaagga cagttgattg ccgaaattga cgacctacct cagtgtaacg
      481 atttgcgtaa tgccgaagct gcactcaacg aagtgaaagc agaacttcaa tctaagcagg
      541 cgctcctgaa acaggctgaa ctgcgtttta aacgccagct ccggatgcta agagaaaatg
      601 ccagctcaca cgaagatttt gagtctgcgg aggctatgct tgcgaccact cgcgctgaat
      661 tacattctct gaatgccaaa ctggtccagg cacagataga agtagataag aaaaagcttg
      721 cccttgaata tacgcgagtc gtagcgccaa tggatggaat tgttattgcc attgtcactc
      781 agcaagggca aaccgttaac tcaaaccaga gtgccccaac aattattaaa ctcgctcgtc
      841 tggatgtcat gaccattaaa gcacagatct ccgaggctga tataacccgt atttctgtgg
      901 ggcaaaaagc ccgcttctct atcttctccg aaccggacaa gcactacagc gcaacactgc
      961 gtgccgttga actggcacca gaatcggtaa tgaaagatga ctctctcgcc agcaacagtt
     1021 cagcatcagg ctccggaact tcaaatgcgt ccgtttatta caatgctcta ttcgatgtgc
     1081 ctaacccaga aaatcgactt cgtattgcca tgactgctca ggttaccctg attactgatg
     1141 aagcccaaaa tacactgctg gtccccattc aggcagtgca cagaaatgag gagaagaagc
     1201 aacaggtcct ggtgctggca gcagatggca ggctggaacc ccggaatgtg aaaactggta
     1261 tcacgaacag cgtggatatt cagatcctcg aaggtttaaa tgtcggagaa aatgtcgtgc
     1321 tgtcgctgcc ggataagaaa gagcccgaag aaaggattat gctgtgaaaa aacttattga
     1381 gttaaaaggg gttagccgaa cttatggcaa cggagatcaa acgcggaccg tcctgaaaaa
     1441 tgtcgatttg acaatcgttg ctggagaaat ggtggcaatc attggagcct ctggctcagg
     1501 caaatcaacc ttaatgaaca taatgggttg ccttgacgta cccaatagag gtgattacta
     1561 catcgacgga caaaatgccg cctgtctttc acccgatgag ctggctagag tgcgccggga
     1621 acacattggc tttatattcc agcgctacca tttgatacct gatcttagcg cacttggtaa
     1681 cgtggagatc ccggccattt acgccaacag cgaacgcgac agccgtcggc aacgtgcaac
     1741 ggcgttgctg ggcagactgg gactggaagg acgtgagcat cataaacctt gcgaactttc
     1801 aggcggtcag cagcaacgcg tcagtattgc ccgagcactg atcaacggtg gaaagataat
     1861 tttggcggat gagccaactg gtgctctgga ttcgcaatcc gggcaagaag tgttagccat
     1921 tctgaatgaa cttaatcgac gtggtcatac tgtagtaatg gtgacccacg atatgaaggt
     1981 cgcccggcac gcaaagcgaa tcattgagct gtgtgatggc gaaatcatcg ctgatagtgg
     2041 aggttgcgta tctgcgacgg aaacactacc aaaaaccaac agaatccggc aaagctattg
     2101 gaaaacactg ctcgatcgaa cgcgtgaatc gatgcaaatg gcgctaaaag cgatgaagac
     2161 gcatcgctta cgcaccacgc ttaccatgat cgggattgtt tttggcatcg cctctgttgt
     2221 cactgttgtg gcattgggtg aaggagccag gcaagaaaca ctggaagaga ttaaaagtct
     2281 ggggacaaac gtggtcagca tttatcccgg gcaggatttg ttcgatgaca gcattgagag
     2341 tattagaaca ttggtacctg ctgacgcaaa tgcactggcg aagcaagggt ttatcgatag
     2401 cgtcagcccg gaagttagcg cttcagacaa cattcgtttt ctgggtaaat ctgcaatagc
     2461 ttccattaac ggcgttgggc gggaacattt ccgggtaaaa ggtattgagt tgttgcaagg
     2521 gagtactttt agagacgatc gcaacgcttt acaggaagtc attattgatg agaatactcg
     2581 taaggccatt ttcgacaacg cagggctaca ggctctgggg caaatagtct ttcttggttc
     2641 tgtaccagca cgaattgttg gcatcgctaa aagtaacaac cgtagtgatg catcaaatcg
     2701 cattactgtg tggatgcctt acagcacggt gatgtaccgt attgtgggta aaccagttct
     2761 gaccggcatt agcgtcaggc taaaagacaa tgtggataac gaggctgcaa tcagcgctat
     2821 ctcccaacta ttaacccggc gtcacggcgt aaaagacttc cagctttata actttgaaca
     2881 aataagaaag tctatcgaac acacctcaat gaccttcagt attttaattc ttatggtcgc
     2941 ctgtatctca ctaatgatcg gtagtatcgg ggtgatgaac atcatgttga tttctgtaac
     3001 ggaacgaact catgaaattg gcgtacgaat ggcggttggc gcgagacgca gcgacattat
     3061 gcaacagttt attattgagg ccgtattggt ttgcctgatt ggtggcgcac tcggtattgc
     3121 actgtcgtat atcacaggtg cactctttaa cgctctggcg ggcgggatat tcgcagctat
     3181 atattcgtgg caggctgctg ttgctgcatt tttttgctca accttaatcg gcataatctt
     3241 cgggtatctc ccagcccgca aggcagcaag gatggacccg gttatttcac tggccagcga
     3301 ataatatatg aaaattttat catcgttgac tttatgcctt atcaccgcac tctgttcagg
     3361 ttgcacaaac gtgctgaaaa gtgactatcg cgctcccgaa gtgaactatc ccatcaactg
     3421 gaccaagggc gatgtggacg gaaatacatc cccgtttgcc tgggaagaat ttaacgatcc
     3481 taatctcgat aactggcttc acctggtgat gacaagtaat aacgacatag ctatagctgc
     3541 actgcggata catcgggcgc agctggatgc ggaaagaaca ggtatcacca atactcctgc
     3601 actaaagggg gcactgagta tggacggaaa aaaacagctg aataactcgt ccggctgggc
     3661 aaaaagcggt tctgccagcc ttggcaccag ctatgagctg gatctctggg gaaaaattgc
     3721 ccgtcagcgt gatgttgctg agtgggcagt ccatgccagt gaagaagatt ttcgctccgc
     3781 gcgcctgatg ctgctttcag aggccagcaa taattactgg cgtatcggtt ttgtaaatca
     3841 acaaattgcc accctccagc agagtattga ctatgcaaaa gagacgctgc gtctggccga
     3901 ggttcgctat cgtgcaggaa atatctcgtc gctggatgtg attgacgcgc agcaaaacct
     3961 gctcacgcaa gagaaccagc ttacagggct acaacgagaa catttacagc tactcaatca
     4021 gcaggccgtt ttgctcggca ccgtgcccgg ttgccagata gtcgagccca caacgctgcc
     4081 aaaaggaagc ctgcctaagg tcaatgcgaa tattcctgcc agcatactga tgcgccggcc
     4141 tgacataagt gctaaagagt ggcagttgcg tgaggcgctg gctacggttg atatcaagcg
     4201 tagcgaatat taccccacct tcaacctgac aggcgctttg ggtaccagca gcgcttcgct
     4261 actggcatta ctgcataatc cgatcggttc cgtaggcgct aacttgacgc taccgttcct
     4321 ggagtggcga cagcgggaca tagaggtaaa aattgcccgt aacgattacg aacagcgagt
     4381 gctggagttt aaacagctac tttataaggc aatgagcagt atagaggacg cgctttcatt
     4441 tcgtaaccag ttgctgcttc aggagacaag actcagggaa gaactggaac ttgcacgtaa
     4501 gtcagagtgg ttaaacgaag ttcgttaccg gcatggtgca gtacgtattt cattctggct
     4561 tgatgctcag gaaagacgcc gtcaggcgga gctacgactg gatgaaaacc gcttcaatca
     4621 gttgcagaat ctggcgaaaa tttatcttga gtttggcggg gcatcaacct ttccttaagt
     4681 ctaattgggg acaaccagga gcaataagga gtgaaaaata gccgcagttg aaaacaccat
     4741 agccattgac atttgagctt taacagctct tgaaaacaaa aaatcactgt taagcgcaat
     4801 gctattcact tcagcagagc agtactgcgt ttcacgagca tgtttaccat tctgtgtaaa
     4861 tgtcttttct taaaagtgat catcaaagcg gtcatcaaac tcgataaata tccaccggca
     4921 tagcgtaatg ccgatcagtt aaggatcaat tgacagctcc ttaaactgcg gcactataac
     4981 ggcttctaca acagggagcc gttttcttat gcaacatttc aatgatctgc tcgatttcag
     5041 tgaccatccg cttatgcctc cggcctctgc acaactgttt gcagaacacc tgccctacga
     5101 gtgaatacag cactgcctga cgctttctgt tcatgcggcc gttcgccgct gtcatttacc
     5161 ggggggacat agttatctgg atggtggtgg tcatggcctt cttccgcaat gagccaatta
     5221 ccgatgttgt tcgccgtctg aagctgagcg cggatggcaa agcggggata aacctgctga
     5281 cccgcagcgt ggtcacccag gcacgtcagc gcgtgggggc cgccccggtg gaatgactct
     5341 tcagccaggc cacacagata tggggcgcgg aacgttacct gaaggataac tggcacggtc
     5401 tccagctttt tgctattgat agcgcgcagt tcagaacatc tgatgaacct aggtgcgtga
     5461 atattatggc tctgccaact catccactga gcggcatagc gcctacctga ttatgcgtct
     5521 ggtaacattg atgaatctgg gaagccacat tttgctgaat gttgtgacca tgccttaccg
     5581 gcggagcgaa accgtgctgg ctcactccat actcgccgcc atcccggata accctattag
     5641 gctgtttgac aagcactttt acaatgcaga cctgctgctg acgctgagcc tgcagggtga
     5701 taaccgccac tggctactac ctgcgtggaa taatatcgca tcagaaacag aagaaagtta
     5761 cgctcccaga tacaggcttc tgaaactgaa ggtgtcactg ccggccaaga aaggaatcct
     5821 gcactgccag agctctggta cgctcgagcg gtaacttatg aggtgaactg tgtggagaaa
     5881 actgtgctga catcactccc ggcagaccgc tacaaagcaa aagaggtggc agagctttac
     5941 tattcacgat gggaaatcga agtcgggtcc agaaacctca aaagcagcca gttgaacaat
     6001 gccctggtgc tgagaagcag tagggttgag gtgttggagc aggaggtgta gggcatgctg
     6061 ctggcttata agctgatacg acgtgaggca accaaagcgg cgtcataaat cagctttaag
     6121 ttcgcgtcca gtttatcgcg acagaaacga tagtgctgag aaatacggca tcaccgggga
     6181 ccatcccgaa acgactggtg catctgcggg tacactggaa gcagtgttca taacgaaacg
     6241 ccccggccat caaggccgaa ggcagttaag atatcaaaaa aacattatcc agtgaaacgc
     6301 agcaccgttc cgcttaagtg aacggcattg ccggcatagc gggagttttt tcaaatg
//
LOCUS       DAJOWB010000197         6339 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJOWB010000197 DAJOWB010000000
VERSION     DAJOWB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.197"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..339
                     /locus_tag="OHO85_004526"
     CDS             <1..339
                     /locus_tag="OHO85_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673783.1"
                     /translation="AAIQKNESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDIMSI
                     PDSSLRKRLGYEVLSFSLQAHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKAT
                     APIQNGPLPA"
     gene            complement(343..1734)
                     /gene="tcyP"
                     /locus_tag="OHO85_004527"
     CDS             complement(343..1734)
                     /gene="tcyP"
                     /locus_tag="OHO85_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCQ2673784.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1867..2457)
                     /locus_tag="OHO85_004528"
     CDS             complement(1867..2457)
                     /locus_tag="OHO85_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCQ2673785.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2620..3288)
                     /gene="hxpB"
                     /locus_tag="OHO85_004529"
     CDS             complement(2620..3288)
                     /gene="hxpB"
                     /locus_tag="OHO85_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCQ2673786.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3435..3971
                     /locus_tag="OHO85_004530"
     CDS             3435..3971
                     /locus_tag="OHO85_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCQ2673787.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4012..4872)
                     /locus_tag="OHO85_004531"
     CDS             complement(4012..4872)
                     /locus_tag="OHO85_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCQ2673788.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4978..5268)
                     /gene="ghoS"
                     /locus_tag="OHO85_004532"
     CDS             complement(4978..5268)
                     /gene="ghoS"
                     /locus_tag="OHO85_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCQ2673789.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5369..6298)
                     /gene="pfkB"
                     /locus_tag="OHO85_004533"
     CDS             complement(5369..6298)
                     /gene="pfkB"
                     /locus_tag="OHO85_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCQ2673790.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGTATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1629 a         1587 c         1555 g         1568 t
ORIGIN      
        1 gctgcgattc aaaaaaatga gagtctccta aagtttcctt cgataatatc aatgcttaat
       61 atcaatcttc tttttaatcc actaaatctt ccgggaatgg gaagtggtgt gcttgaagat
      121 attatgagta ttccagatag ttcacttaga aaacgtctcg ggtatgaggt tttaagcttc
      181 tcactacagg cacattcatt gagccaggaa tgtattgata agttggacat ttttttcgct
      241 gacgatctgt ttaaatatga gtcagtatgc atcgccgcga tggaacattt aaaaagtaaa
      301 gcaacggccc ctatacaaaa cggaccgttg ccagcataag aattaacggt gtgccagttc
      361 ggcgtcgtct tcgctatcca gaatggcttt atcggtttgc ttcagccact ggctggtcag
      421 cgtgccagct gtcatcgagc cactaacgtt tagcgccgta cggcccatgt cgataagcgg
      481 ttcaacggag attaacagcg ccaccagggt tactggcagg cccatcgcag gcagcacaat
      541 cagtgcggcg aaagttgcac caccaccgac accggcaacg cctgcggaac taacggtaac
      601 aataccgacc agcgtcgcaa tccacatcgg gtccagcggg ttaatgccaa ccgtaggcgc
      661 aaccatcacc gccagcattg ccggatacaa accggcgcag ccgttctgac caatggtcgc
      721 accgaaagag gcggcgaaac tggcgatgga ttcaggaacg cccagacgac gcgtttgtgc
      781 ttccacattc agtgggatag acgcagcact ggaacggctg gtaaaggcaa acgtcagcac
      841 aggccatacc ttacggaagt acttcagcgg actcacgcca ttaatgccca gcagaatgcc
      901 atgcactgca aacataatca gcagaccgag gtaggacgcg acaacgaaac ttcccagttt
      961 gatgatgtct tgcaggttag aacctgcaac cactttggtc attagtgcca gaacgccgta
     1021 aggggtcaac tgcatgacca ggcgaaccag tttcatcacc cagctttgca gggtatcgat
     1081 agcggttaag acgcgttcac ctttcggcgc atcatccttc agcagtttca gcgcagctac
     1141 accgaggaat gcggcaaaaa ttaccacgct gataattgac gtcggattgg ctccggtcag
     1201 atcggcaaac gggtttttcg ggataaagga caagaccagc tgcggaacgc tcaggtcaga
     1261 gactttacca acatagttac tttcgatggc gttcagacgt gcagtttctg caccaccctg
     1321 aaccagacct tcagccgtca aaccaaacag gttggtgacc agcacaccga ccagcgccgc
     1381 aatcagtgtg gtaaacaaaa gcgtaccgat ggtcagaaaa ctgattttgc ctaactgaga
     1441 tgcgttatgc agacgggcaa ccgcgctcag aatagaggcg aagactaacg gcataacgat
     1501 catttgcagc agttgaacat agccgttacc aacgatgtta aaccactgta cagaatcttt
     1561 aagtacctgg ctgtcagaac cataaatggt atgcagggca aggccaaaaa ccacacccat
     1621 caccagaccc accagcactt ttttcgccag actccactgt ttgtggcggg tctgagccag
     1681 cgcaaacagc agtacaacga acaccacgat gttcgcaatt aatggaaagt tcatccccgt
     1741 tctcctgatc ttattatttc tatcactgcg tagcagtcat agtgttggcg caagattaac
     1801 agaactgtga gctggctctt atattcaaat ggaattgctt attccagaaa ggtgattttt
     1861 gcctttttag tattcaacct gatgcttaat aagccgatga aactggatct gcaaggtatt
     1921 gatcatttgc gatgaccaac gaaccgcgct gttttcgggt aatgaatggg gcatccatac
     1981 cgaccagaca aataatgcca tgcagataaa acgttccagt tggttgccct tttggggaac
     2041 caggataggc aagcggaaac gccagcgaca tggccagagc aggggaacac cggcgggtgt
     2101 cagcatatcg gcaagtatgt ggcttaaata acccagcacc attccttgta gcgcatcagc
     2161 cggaatgaac cagctttcag gaaccttaag gtaaaaggtt gccagcagcg caaataccgc
     2221 cagcagactg tgggtaaaac cacgatgccc aaaagcgcgg gcgatcggtt ttgatatcca
     2281 ctttaatcgc tgcccaagaa acgactttgg gtgatcgatg tccggtaaca aacacgtcag
     2341 gattgcggaa gggacaatat gccaccagtc accctgtgcc agcacgggcg tcagctcggc
     2401 atttttggca aataccgcac aagcaataga aaagagaagg tgaccttccg ccgtcatgat
     2461 aaaactcact aaactgtcga tacgtacagt ataggatttt tatacagtgc acggaagtgg
     2521 tgacggtgta actctttctc ttaaattgac gattaatccg tcagttgaat gcaaattcag
     2581 ataaagaacc aggggcagcg agatcactgc ccctggtcat taaccgagaa ggtcttttgc
     2641 ggtgagttct gtcagcgatg agagtttgac gtttgctaat acaaaacgtg gatcattttg
     2701 cgcttctggc gcaggaacga caatggaacg catgcgggct gctttagagg cgatcatgcc
     2761 atttactgaa tcttccagcg ctacgcaggt cagtgggtca acgcccagtt ttgctgcgca
     2821 gtcgagatat acttgcggat gcggcttgct gtaaggcagt ttttcagccg aggcgagggc
     2881 atcgaaactg tcgcgtaagt caaacatggt caacactttt tccagcatat gtagtggtga
     2941 cgcggaggcc agtcccacca ataaaccttg ttctttgcat aacgcaacgg cttcgcgcac
     3001 gcctggtaat aatggacgtg tctcttcaac cagtgaaatg gcacgggcaa taacccgttc
     3061 tactacttcc tgacggcttg gcccattcca cggttgccgg gcgtaccaaa gatcgaccac
     3121 catatcgatg cgtaaaccta aggtgtccgg cagctcgtta cgacgggaga tatccacccc
     3181 caggcttgcc atcacatcca gttcggctcg atcccataaa ggttctgagt cgataagtaa
     3241 tccatccata tcaaaaattg cagcaagaat ctgacgcggg gttgacatta caacctctcc
     3301 tttagagcat tatgtgtaag gtattaagcc gtgcttatga ttaagcgcct atcatatcgc
     3361 ctttaaagaa tgggcgaaaa cagatatcta caggtaaact aagtgaaacg acgcgtctta
     3421 atcaaggaga actcatgacg tatcaacaag ctggacgcat tgctgttttg aaaaggattt
     3481 tgggttgggt gatttttata cctgcattga tctctacatt gatttctttg ctgaaattta
     3541 tgaatacccg gcaggaaaac caggaaggca ttaatgcggt catgctcgac tttactcatg
     3601 tcatgatcga tatgatgcag gcgaatacgc cttttttgaa tctgttctgg tataactccc
     3661 cgacgcccaa ttttaatggt ggcgtgaacg ttatgttctg ggtgattttc atcctgattt
     3721 ttgtcggact ggcgttgcag gattccggtg cccggatgag tcgccaggcg cgcttcttgc
     3781 gagaaggtgt tgaagatcaa ctcattctgg aaaaagccaa aggggaagag gggctaacgc
     3841 gtgaacaaat tgaatcacgt attgttgttc cgcaccatac tattttcctg cagtttttct
     3901 cactgtatat cctgccggtt atttgtattg ctgctggtta tgtattcttt tctctgcttg
     3961 ggtttattta atcaaaaggc cgcacggagc ggccattatc ttcaacccat atcatgctgc
     4021 taataatcta tccagtgact gctgagcaat aaccaaatgc tgaccgccaa ataaccttgc
     4081 acgatttaac agcgtgtaga gttggtaaac cggttgacgt tcaaggaaat cggcaggtag
     4141 cggggatact gactgatagc cgtcatagat ttgtggcggc tgttctgtat gcagcggtaa
     4201 catcgccagg tcgcactctc ggtcacccca gtagcaggcc gggtcgaaaa tgtacgggcc
     4261 atccggaccc agcgcacagt tgccggacca taaatcgccg tgcaacagag aaggctgcgg
     4321 ttgatgtgag gcgagacgct gctgaatatg ctctacgatg gcgtcgatat tgccgaaagc
     4381 gatccctttt tctgctgcca gttccagctg ccagccaatc cgttgttcag caaaaaacgt
     4441 tgaccagcga cgttgccagg tgttgggctg tggagttgtg gagagtgcgt tatcgaaatc
     4501 gaggccaaat tgtggttggt cgctccattg atgtaaacgc gctatttgct gaccaagaat
     4561 aaatgcgcta tgcgcatcca gcggacgagg tgggagataa tccatcacca gaaaactgta
     4621 gtcacggtca gcgcctactg cccaaacctt aggcacggtg acggttttac tacgcgacag
     4681 taactccagt tggtcggctt cggcggtaaa accgggaagc agttcccttt catcacattt
     4741 gacgaaaaag tcatgtcctg catagcgcaa atgccatgcg gcatggactt ctccgccagg
     4801 cagttcatta cgcagttcga tttcgccttc acctaactgc tcgctcaaaa gacgactgat
     4861 tgcctgccac atgatgtctc tcccatgttg tctgccagat cataaggtta gcgcaagaat
     4921 gcggtgtaaa aacacgagct aacgcacacc acagactgat tattgcccaa aaaccattta
     4981 ttttctgttg ctattccatt cctcccaggt cgtgatggtg atttcaggat ctttgccggt
     5041 tgcactttgg gtaagccccg aaaccagttc tctaatttct tcttcacttt gggtactgat
     5101 aagtccaaaa gtgttagtcc ccagctcatg gatatttccc tcatcatccg tcatggtgag
     5161 caaaaaaccg tcgcgagtca ggtaattatt caactcgtta atttcagtca atgtatcctc
     5221 atgcaacatg acggtaatga cataacggac aagatcgcca ctggccatag ttcacccctt
     5281 tgttatcaca tgacttttga gcatagtcgg agaaacgcgt tgccgacagg ttggtgatga
     5341 ttcccccaat gctgggggaa tgtttttgtt agcgggaaag gtaagcgtaa attttttgcg
     5401 tatcgtcatg ggagcacaga cgtgttccct gattgagtgt ggctgcactc cccgcagcta
     5461 cgccaaaacg aaccatctct tcaagagagg cattttctgc cagtttcagt gtcatcgcgc
     5521 cgaccatgct gtcaccagcg ccaacggtac tctggctttt caccggtggt ggcaccacct
     5581 gaatgcagtt ttcactatca acacccagcg ctccttgtgg acccagggaa acgacaaccc
     5641 gtttggcctt gccgctatta acgatttcct gcgcggcttt gcggacatcg tctggctggg
     5701 tgagttcgcg attcaccagc gcactgagtt ctttttggtt aggcttaacc aactcgatgt
     5761 taccaatcgc cagtgctgca cttaacgctt cgccagaact gtcgacgatg cagcggatcc
     5821 cttgtttttg cgcagcggaa atcagttggg ttaatttttc cagcttcaca cctggcggca
     5881 ggcttccgct tatgaccagg atggccccgg attcaatttc cagaacttgc tcttcaagct
     5941 ggcgaaactc atcttcattt aatgccgcgc ctggcataac aaaacgatac tgctcaccgc
     6001 ttgcttccac atgtacatgt aaattctgcc gggtccagtc tttggcttct acagtagcga
     6061 cggggacatt ttcatccgcc aacagtgaaa ccagatgttc gccggtcgcg ccacccgccg
     6121 ggaagatcgc tgtggcagtg cctccaagat gggcaatggc gcgggcgacg ttgatgccgc
     6181 cgcccccggg ttcgaacacc ggtgcggtac agcgcagttt tccttcggga taaatttgcg
     6241 gggtaattgt tgcgctatcg agagagggcg caagtgtcaa cgtatagata cgtaccatca
     6301 tttcctccta taggctaatt tcagtctggc accgaatca
//
LOCUS       DAJOWB010000198         6330 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJOWB010000198 DAJOWB010000000
VERSION     DAJOWB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.198"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..536)
                     /locus_tag="OHO85_004534"
     CDS             complement(<1..536)
                     /locus_tag="OHO85_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCQ2673791.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPD"
     gene            685..1698
                     /gene="intI1"
                     /locus_tag="OHO85_004535"
     CDS             685..1698
                     /gene="intI1"
                     /locus_tag="OHO85_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCQ2673792.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            1901..2251
                     /locus_tag="OHO85_004536"
     CDS             1901..2251
                     /locus_tag="OHO85_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCQ2673793.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2377..2937
                     /locus_tag="OHO85_004537"
     CDS             2377..2937
                     /locus_tag="OHO85_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCQ2673794.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            2940..5906
                     /locus_tag="OHO85_004538"
     CDS             2940..5906
                     /locus_tag="OHO85_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCQ2673795.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            5915..>6330
                     /locus_tag="OHO85_004539"
     CDS             5915..>6330
                     /locus_tag="OHO85_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004026565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673796.1"
                     /translation="MFRFLRRPLAEVANYSNDRWEAPQRASRLAASVKRYKTSEMLRF
                     IFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVFGEKGRQHRSADSNPEMVEK
                     MAARYRHAAELHWSATLAEIERVKRLYQTKIKKSKK"
BASE COUNT         1313 a         1956 c         1842 g         1219 t
ORIGIN      
        1 cagtcgggcg gcgagttcca tagcgttaag gtttcattta gcgcctcaaa tagatcctgt
       61 tcaggaaccg gatcaaagag ttcctccgcc gctggaccta ccaaggcaac gctatgttct
      121 cttgcttttg tcagcaagat agccagatca atgtcgatcg tggctggctc gaagatacct
      181 gcaagaatgt cattgcgctg ccattctcca aattgcagtt cgcgcttagc tggataacgc
      241 cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta cagcgcggag aatctcgctc
      301 tctccagggg aagccgaagt ttccaaaagg tcgttgatca aagctcgccg cgttgtttca
      361 tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac tgtgtggctt caggccgcca
      421 tccactgcgg agccgtacaa atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg
      481 acgccaacta cctctgatag ttgagtcgat acttcggcga tcaccgcttc cctcatgatg
      541 tttaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc
      601 aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtacc
      661 ccaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt
      721 tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta cagcttacga
      781 accgaacagg cttatgtcca ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac
      841 ccggcaacct tgggcagcag cgaagtcgag gcatttctgt cctggctggc gaacgagcgc
      901 aaggtttcgg tctccacgca tcgtcaggca ttggcggcct tgctgttctt ctacggcaag
      961 gtgctgtgca cggatctgcc ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc
     1021 ttgccggtgg tgctgacccc ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag
     1081 catcgtttgt tcgcccagct tctgtatgga acgggcatgc ggatcagtga gggtttgcaa
     1141 ctgcgggtca aggatctgga tttcgatcac ggcacgatca tcgtgcggga gggcaagggc
     1201 tccaaggatc gggccttgat gttacccgag agcttggcac ccagcctgcg cgagcagctg
     1261 tcgcgtgcac gggcatggtg gctgaaggac caggccgagg gccgcagcgg cgttgcgctt
     1321 cccgacgccc ttgagcggaa gtatccgcgc gccgggcatt cctggccgtg gttctgggtt
     1381 tttgcgcagc acacgcattc gaccgatcca cggagcggtg tcgtgcgtcg ccatcacatg
     1441 tatgaccaga cctttcagcg cgccttcaaa cgtgccgtag aacaagcagg catcacgaag
     1501 cccgccacac cgcacaccct ccgccactcg ttcgcgacgg ccttgctccg cagcggttac
     1561 gacattcgaa ccgtgcagga tctgctcggc cattccgacg tctctacgac gatgatttac
     1621 acgcatgtgc tgaaagttgg cggtgccgga gtgcgctcac cgcttgatgc gctgccgccc
     1681 ctcactagtg agaggtaggg cagcgcaagt caatcctggc ggattcacta cccctgcgcg
     1741 aaggccatcg gtgccgcatc gaacggccgg ttgcggaaag tcctccctgc gtccgctgat
     1801 ggccggcagc agcccgtcgt tgcctgatgg atccaacccc tccgctgcta tagtgcagtc
     1861 ggcttctgac gttcagtgca gccgtcttct gaaaacgaca atggaggtgg tagccgaggg
     1921 tgtggaaaca cccgactgcc ttgcgtggtt gcggcaggcg ggttgcgaca cggtgcaggg
     1981 tttcctgttc gccaggccga tgccggcggc ggccttcgtc ggcttcgtca accaatggag
     2041 gaacaccacc atgaacgcca atgaaccgag caccagttgc tgcgtgtgct gcaaggaaat
     2101 cccgctcgat gccgccttca cgccggaagg ggccgagtac gtggagcatt tctgcgggct
     2161 ggagtgctat cagcgcttcc aggcgcgggc cagcactgcg accgaaacca gcgtcaaacc
     2221 ggacgcttgt gattcgccgc cgtcaggttg aggcataccc taacctgatg tcagatgcca
     2281 tgtgtaaatt gcgtcaggat aggattgaat tttgaattta ttgacatatc tcgttgaagg
     2341 tcatagagtc ttccctgaca ttttgcaggg aattccatga ctggacagcg cattgggtat
     2401 atcagggtca gcaccttcga ccagaacccg gaacggcaac tggaaggcgt caaggttgat
     2461 cgcgctttta gcgacaaggc atccggcaag gatgtcaagc gtccgcaact ggaagcgctg
     2521 ataagcttcg cccgcaccgg cgacaccgtg gtggtgcata gcatggatcg cctggcgcgc
     2581 aatctcgatg atttgcgccg gatcgtgcaa acgctgacac aacgcggcgt gcatatcgaa
     2641 ttcgtcaagg aacacctcag ttttactggc gaagactctc cgatggcgaa cctgatgctc
     2701 tcggtgatgg gcgcgttcgc cgagttcgag cgcgccctga tccgcgagcg tcagcgcgag
     2761 ggtattgcgc tcgccaagca acgcggggct taccgtggca ggaagaaatc cctgtcgtct
     2821 gagcgtattg ccgaactgcg ccaacgtgtc gaggctggcg agcaaaagac caagcttgct
     2881 cgtgaattcg gaatcagtcg cgaaaccctg tatcaatact tgagaacgga tcagtaaata
     2941 tgccacgtcg ttccatcctg tccgccgccg agcgggaaag cctgctggcg ttgccggact
     3001 ccaaggacga cctgatccga cattacacat tcaacgatac cgacctctcg atcatccgac
     3061 agcggcgcgg gccagccaat cggctgggct tcgcggtgca gctctgttac ctgcgctttc
     3121 ccggcgtcat cctgggcgtc gatgaactac cgttcccgcc cttgttgaag ctggtcgccg
     3181 accagctcaa ggtcggcgtc gaaagctgga acgagtacgg ccagcgggag cagacccggc
     3241 gcgagcacct gagcgagctg caaaccgtgt tcggtttccg gcccttcacc atgagccatt
     3301 accggcaggc cgtccagatg ctgaccgagc tggcgatgca aaccgacaaa ggcatcgtgc
     3361 tggccagcgc cttgatcggg cacctgcggc ggcagtcggt cattctgccc gccctcaacg
     3421 ccgtcgagcg ggcgagtgcc gaggcgatca cccgtgctaa ccggcgcatc tacgacgcct
     3481 tggccgaacc actggcggac gcgcatcgcc gccgcctcga cgatctgctc aagcgccggg
     3541 acaacggcaa gacgacctgg ttggcttggt tgcgccagtc tccggccaag ccaaattcgc
     3601 ggcatatgct ggaacacatc gaacgcctca aggcatggca ggcactcgat ctgcctaccg
     3661 gcatcgagcg gctggttcac cagaaccgcc tgctcaagat tgcccgcgag ggcggccaga
     3721 tgacacccgc cgacctggcc aaattcgagc cgcaacggcg ctacgccact ctcgtggcgc
     3781 tggccaccga gggcatggcc accgtcaccg acgaaatcat cgacctgcac gaccgcatcc
     3841 tgggtaagct gtttaacgct gccaagaata agcatcagca gcagttccag gcgtcaggca
     3901 aggccatcaa cgccaaggta cgtctgtacg ggcgcatcgg tcaggcgctg atcgacgcca
     3961 agcaatcagg ccgcgatgcg tttgccgcca tcgaggccgt catgtcctgg gattcctttg
     4021 ccgagagcgt caccgaggcg cagaagctcg cgcaacccga tgacttcgat ttcctgcatc
     4081 gcatcggcga gagctacgcc accctgcgcc gctatgcacc ggaattcctt gccgtgctca
     4141 agctgcgggc cgcgcccgcc gccaaaaacg tgcttgatgc cattgaggtg ctgcgcggca
     4201 tgaacaccga caacgcccgc aagctgccag ccgatgcacc gaccggcttc atcaagccgc
     4261 gctggcagaa actggtgatg accgacgccg gcatcgaccg gcgctactac gaactgtgcg
     4321 cgctgtccga gttgaagaac tccctgcgct cgggcgacat ctgggtgcag ggttcacgcc
     4381 agttcaagga cttcgaggac tacctggtac cgcccgagaa gttcaccagc ctcaagcagt
     4441 ccagcgaatt gccgctggcc gtggccaccg actgcgaaca atatctgcat gagcggctga
     4501 cgctgctgga agcacaactt gccaccgtca accgcatggc ggcagccaac gacctgccgg
     4561 atgccatcat caccgagtcg ggcttgaaga tcacgccgct ggatgcggcg gtgcccgaca
     4621 ccgcgcaggc gctgatagac cagacagcca tggtcctgcc gcacgtcaag atcaccgaac
     4681 tgctgctcga agtcgatgag tggacgggct tcacccggca cttcacgcac ttgaaatcgg
     4741 gcgatctggc caaggacaag aacctgttgt tgaccacgat cctggccgac gcgatcaacc
     4801 tgggcctgac caagatggcc gagtcctgcc ccggcacgac ctacgcgaag ctcgcttggc
     4861 tgcaagcctg gcatacccgc gacgaaacgt actcgacagc gttggctgaa ctggtcaacg
     4921 ctcagtttcg gcatcccttt gccgggcact ggggcgatgg caccacatca tcatcggacg
     4981 gacagaattt ccgaaccgct agcaaggcaa agagcacggg gcacatcaac ccaaaatatg
     5041 gcagcagccc aggacggact ttctacaccc acatctccga ccaatacgcg ccattccaca
     5101 ccaaggtggt caatgtcggc ctgcgcgact caacctacgt gctcgacggc ctgctgtacc
     5161 acgaatccga cctgcggatc gaggagcact acaccgacac ggcgggcttc accgatcacg
     5221 tcttcgccct gatgcacctc ttgggcttcc gcttcgcgcc gcgcatccgc gacctgggcg
     5281 acaccaagct ctacatcccg aagggcgatg ccgcctatga cgcgctcaag ccgatgatcg
     5341 gcggcacgct caacatcaag cacgtccgcg cccattggga cgaaatcctg cggctggcca
     5401 cctcgatcaa gcagggcacg gtgacggcct cgctgatgct caggaaactc ggcagctacc
     5461 cgcgccagaa cggcttggcc gtcgcgctgc gcgagttggg ccgcatcgag cgcacgctgt
     5521 tcatcctcga ctggctgcaa agcgtcgagc tacgccgccg cgtgcatgcc gggctgaaca
     5581 agggcgaggc gcgcaatgcg ctggcccgtg ccgtgttctt caaccgcctt ggtgaaatcc
     5641 gtgaccgcag tttcgagcag cagcgctacc gggccagcgg cctcaacctg gtgacggcgg
     5701 ccatcgtgct gtggaacacg gtctacctgg agcgtgcggc gcatgcgttg cgcggcaatg
     5761 gtcatgccgt cgatgactcg ctattgcagt acctgtcgcc actcggctgg gagcacatca
     5821 acctgaccgg tgattaccta tggcgcagca gcgccaagat cggcgcgggg aagttcaggc
     5881 cgctacggcc tctgcaaccg gcttagcgtg ctttattttc cgttttctga gacgacccct
     5941 ggctgaagtg gctaattaca gtaacgaccg ctgggaagca ccgcaacgtg cctcacgcct
     6001 ggctgccagc gtaaagcgtt acaagacatc agaaatgctg cgctttattt ttgctaccat
     6061 tgcctacgat cccgatccag atttaacccc gctgactgtg agacgtttgt gcaaggcatt
     6121 gttcggcaga accggaagcc agtggctggt tgttgaagtt tttggggaga aggggcgtca
     6181 gcaccgcagc gcggacagca atccggagat ggtggaaaaa atggctgcca gatatcgtca
     6241 tgccgccgaa ctacactggt ccgcaacgct ggcagaaatt gaacgggtaa agcggcttta
     6301 tcagacgaaa attaagaaat ccaaaaaata
//
LOCUS       DAJOWB010000199         6323 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJOWB010000199 DAJOWB010000000
VERSION     DAJOWB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.199"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..409)
                     /locus_tag="OHO85_004540"
     CDS             complement(<1..409)
                     /locus_tag="OHO85_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="RepA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCQ2673797.1"
                     /translation="MADLLQKYYSQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSKGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRASRALTFLAELGLITYQTEY"
     gene            complement(402..884)
                     /locus_tag="OHO85_004541"
     CDS             complement(402..884)
                     /locus_tag="OHO85_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673798.1"
                     /translation="MANHETHSVIAGGIESDLHIDSSKYPHPFCSLLQKRAHFDSFKH
                     LIFIRGLLAIAHGKRKNKISASMVSFYVSLVQVMCAHYIPDTNKVNLSVKELSVCSGG
                     SYTRVWRALKTLDNDLHLIAFDGGTIWFRPDMFETLRVGPDELVTARRRGNSVGGGHG
                     "
     gene            complement(877..951)
                     /gene="tap"
                     /locus_tag="OHO85_004542"
     CDS             complement(877..951)
                     /gene="tap"
                     /locus_tag="OHO85_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCQ2673799.1"
                     /translation="MPGKVQDFFLCSLLLCIVSAGWCG"
     gene            complement(1183..1440)
                     /gene="repA"
                     /locus_tag="OHO85_004543"
     CDS             complement(1183..1440)
                     /gene="repA"
                     /locus_tag="OHO85_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCQ2673800.1"
                     /translation="MSQTENAVTSSSGTKRTYRKGNPVPARERQRASLARRSNTHKAF
                     HAVIQARLKDRLSELADEEGITQAQMLEKLIESELKRRATS"
     gene            complement(1724..1930)
                     /locus_tag="OHO85_004544"
     CDS             complement(1724..1930)
                     /locus_tag="OHO85_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCQ2673801.1"
                     /translation="MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRE
                     RLCELNIHRGNTVVQVTLAYEARK"
     gene            complement(2234..2308)
                     /locus_tag="OHO85_004545"
                     /pseudo
     CDS             complement(2234..2308)
                     /locus_tag="OHO85_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
     gene            complement(2554..2766)
                     /locus_tag="OHO85_004546"
     CDS             complement(2554..2766)
                     /locus_tag="OHO85_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCQ2673802.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     IAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            complement(2902..3462)
                     /locus_tag="OHO85_004547"
     CDS             complement(2902..3462)
                     /locus_tag="OHO85_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCQ2673803.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAGRLEKIRRQNRTKAELQAVLDGK"
     gene            complement(3517..4263)
                     /gene="traX"
                     /locus_tag="OHO85_004548"
     CDS             complement(3517..4263)
                     /gene="traX"
                     /locus_tag="OHO85_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCQ2673804.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQSWMFLVGRGAFPLFALVWGLNLSRHTHIRQEAINRLWGWAVIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRTWWRSAETMLLLAMWLPFSGTSYGIAG
                     LLMLAVSHRLYRAEDRTERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            complement(4283..>6323)
                     /locus_tag="OHO85_004549"
     CDS             complement(4283..>6323)
                     /locus_tag="OHO85_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCQ2673805.1"
                     /translation="TDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAA
                     DVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWVPEHSVTEF
                     SHSQEAKLAEAQQKAMLKGEAFPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDR
                     RVLNSMIHDAREKAGELGKEQVMVPVLNTANIRDGELRRLSTWETHRDALALVDNVYH
                     RIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRE
                     RGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIGLAYAITAHGAQGASET
                     FAIALEGTEGNRKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDV
                     LEPKSDREVMNAERLFSTARELRDVVAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYV
                     ALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLA
                     DNMQDGVRIARDNPDSGVVVRIAGEGRPWNPRTITGGRVWGDIPDNSVQPGAGNGEPV
                     TAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQAVRDIAGLERDRSAI
                     SEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDMVRDLQKEKTLGGD"
BASE COUNT         1367 a         1904 c         1533 g         1519 t
ORIGIN      
        1 catattccgt ctggtaggta atcagtccca gctctgcaag gaacgtcaag gctcgggagg
       61 cccgggtgat ggagagtttt ccggcagcag actccgtcgc cagtccgcac tcaatggcca
      121 gcgtagtgat ggagcactgg acgcggttgg ccagcgggtc atagtgaaaa cacagcccct
      181 gcagcagcgc atcaatagcc cggcgacgca gtaccggtgg catgcgccga cgcagtccct
      241 tcgaacgggc atgtgccaca tgaatggcga aatcaaaacg ggaggtgaag cccaccgcct
      301 tttccatcag tttttcgcag aacttcagcg ttccggcacc ttcacggggt gtgaacaccg
      361 gattcggatt tttaacctgt gaatagtatt tttgaaggag atcagccatg tcccccacca
      421 acactattcc ccctcctacg ggcggtaact agctcgtctg ggccgacacg taaagtttcg
      481 aacatatctg gtctgaacca gatggttcct ccatcaaaag cgatgagatg aagatcatta
      541 tcaagagttt tgagggctct ccaaacacga gtgtatgaac ctccactaca aactgaaagc
      601 tctttaacag ataagttaac tttgttcgta tcagggatgt agtgtgcgca cattacttgt
      661 acgagggaaa cataaaaact aaccatcgac gcgctgattt tatttttacg tttgccatgc
      721 gctattgcaa gcaaacctct gatgaagatc agatgcttga aggagtcaaa atgagccctt
      781 ttctggagaa ggctgcaaaa cgggtgagga tacttgctgg aatcaatgtg aagatctgat
      841 tcaatcccac ccgctattac tgaatgtgtt tcatgattag ccacaccatc ctgcacttac
      901 aatgcacaga aggagtgagc acagaaagaa gtcttgaact tttccgggca tataactata
      961 ctccccgcat agctgaattg ttggctatgc ggttttatgg ggccccggta atctttctag
     1021 tcgccaaact tgaagaagat tatcggggtt ttcgcttttc tggctcctgt aaatccacat
     1081 cagaaccagt tccctgccac cttacggcgt ggccagccac aaaattcctt aaacgatcag
     1141 caatctacca ctcactcctg agataagcaa gaatgtgaat atttacgaag tcgctctgcg
     1201 tttcagctct gattcaatca gtttttcaag catctgcgcc tgagtaatac cttcctcatc
     1261 cgccagttca ctcagcctgt cttttaaccg ggcctgaata accgcatgaa aagccttatg
     1321 agtgttgctt ctgcgagcca gagaagctct ttgcctctct ctggccggaa cagggttacc
     1381 tttccggtat gtacgcttgg tgcctgatga ggaagtcact gcattttctg tctgcgacat
     1441 ctcgcctcct caattctcaa acagggatcg tttcgcagag aatactacag ttttttgaaa
     1501 tcagcgactt gaaagttgtg acgatgatct gtgttggcgc acaaatcatg tctgattacg
     1561 accgtttaat gtgattttga taatgaaatg actgaaaagc tggaaatgcc ttagccaggg
     1621 tgccggcccc gggcgggacc ggctgtttct ggtatatagg gtgtctgccc ctgaaatacc
     1681 tcacacttcc ggaaagcggg gacttccgtc cccgcccggc agcttacttc cgtgcttcgt
     1741 aggccagagt tacctgcacc actgtatttc ctctgtgaat attcagctca cataaccgtt
     1801 ccctgaatat cagtgaaaaa cacaacaccg tggcgcacac ggcgagcaac ccgataaggg
     1861 catatttcgt cataaacttt gacctctcag caaagaagag gctacaatca gtagtgttaa
     1921 ggtacttcat gcagacctca cgagttaatg gattaacgag tggggtcttc gcatttctgg
     1981 tacctgcgtc ttgcctgaac cagaaaggct caaacacccg ccgcgataat accacattcc
     2041 ctgttgctga ctattacccc catttgcctg tttttgttac aaaaacgccg ggagcggggg
     2101 aaaacttact ttttcccgtt tccggggggg acaactgagc attgcgaacg cgtcagctac
     2161 gatgttccgg tgattgctga tcagcccctg ttgtattcat aaactaatta acattcattc
     2221 tgtctggtgc ataaatctgc ccgtgaacat ctgcagacaa taccggccat gaaaagataa
     2281 ataataccag tggaatatat ttcctcatgt tattctccat atcagataaa tggcacctgt
     2341 atttcagccc ccctgaatat cgcagcacac attgaataag accttatttt gtgaaattaa
     2401 tcacaaaata taaaatcgcg acttaatacg gaaaaaactc tgtgctactc agaactggcc
     2461 ggtcatactg cccgaccgga tattaaaaaa agcaaatctc ataaactcct ttcagccttc
     2521 tgactcctgg agtaatgaag gctgcttttt tcgttacacc cttccccggc agcgccggtg
     2581 gcagtgttca gcccttttcc gggcaaactg ttgccagtct gtccggggag ctatgcaggc
     2641 agcccgtcgc catgcttctg ccgcttccat ccagcgtcca ctgacctcca gaataacggc
     2701 ccgcccgccg gcacgctggt aacgccagac ctgaccgttc cgtgaactgt ttctgaatcc
     2761 attcattttt ctgctcctcc cttttagtcg tcagtattat tgtggtaaat aattcagtgg
     2821 actggcttcc tttcgggtac ggaagttatt tccttttaag ccttattgtt tcagcctccc
     2881 cggtttccgg ggaggctttt tttatttccc atcaagcacg gcctgaagtt ctgcctttgt
     2941 ccggttctgg cggcgaattt tctccagacg ccctgccgca tacgcttcct cctcctgaga
     3001 aatatgctcc gtcacatacc cttccgtgtc ataccggcag gcaccggctt tcatggcaca
     3061 cagatagctt tctgaacggg tgatggcctt cagcgccctg cgcagttttt tatgcgagag
     3121 cgggatattc cgctgcgcca cgtcttccag taacacgtcc cggataccgc aggccagaag
     3181 ccggggggtg tcaccgtcaa ataatcccgg ccaccagggc ttcagggtat tcacggcctc
     3241 atccagcgtg ggcaggttca gataaatgga cagtgcctgt ctggcctgcg ctttttttgc
     3301 cgccagctca gcttcccggg ccgctttctc cgccagcttc tgctttttca ccttccattt
     3361 tggtggcgtg gtgacattga tgatggtttt ccggctgcgg acaggcgtct ctccttctgt
     3421 tttccgcttc agtgtcagta ccggtcgctt ctgctctgtc atgctgtgtt ctgcctctga
     3481 cgaatcatcc tgttacggtc aaagatacca cacccgttac agcgccagaa cgcccagcac
     3541 agccagatga caggcataga acgtcgggaa aaaatctccg ggccagaaac ggggtaatga
     3601 tttccctgca caggacacca gaccaacggt cagcaccgtc atcacaaggc cggctacagc
     3661 cgccgcatca ctggtagcaa ggttaagcgc cggaatcacg gccagcaggc atgcaaccag
     3721 cgccagacgt tccgttctgt cttccgcccg gtacagccgg tggctgactg ccagcatcag
     3781 cagcccggca atgccgtaac tggtgccgga gaaaggcaac cacattgcca gaagcaacat
     3841 ggtctctgcg gaacgccacc aggtgcgcgt ttcacaccat gtcagcacct gtgccgcaac
     3901 tgcaaaggca aacaggatat tcccctcata ccagggaaag ccggccagat aatatgcgaa
     3961 ctgggcaatc accgcccatc cccacagccg gttaatggcc tcctgccgga tatgcgtatg
     4021 acgggacaga ttcagccccc acaccagggc aaacagcgga aaggccccac gcccgaccag
     4081 aaacatccac gactggtcca gatgcagaat gcggtttgca tggtccagaa ccatcagcac
     4141 cagtgccacc gttttgatga tatcccgctg tccgggtgac cagaccagca acgactgcaa
     4201 ccaggtatcg gtccgggcag ccagtgaatc attacgtgtt gtgttcgtgt tatccgttgt
     4261 catcagcgtt tatcttcctg tgtcagtctc cgcccagggt tttctctttc tgcaggtcac
     4321 gaaccatatc ccgctccatc tgctgcagtc gctcctgcag cagattttcc cgggcaacct
     4381 cccggaccgc ttcccgcacc cgttgtggtt cacgcagtac actctccggc agcgcggctt
     4441 cccgttcaga tatggcagac cggtcacgct ccagcccggc aatatccctg acagcctgct
     4501 ctgttttgcc gtccggcagg tcaggtttgt tctctgccat tttacggaca atttcatccg
     4561 cacggcgttc cgtttcacgg cggatggcct cttcagcctg ccgctgtgcc agtacctctg
     4621 ccgtgaccgg ttcgccattt ccggctcccg gctgaacgct gttgtccggg atatcccccc
     4681 acacgcgacc accggtgatg gttctgggat tccacggacg accttcaccg gcgattctta
     4741 ccaccacacc gctgtcagga ttatcacggg caatccggac accatcctgc atattatcag
     4801 ccagcaggct ctctccgtta cggctgccct gcagggccac gaactgcgca tccccgctgc
     4861 ctttcacccg gccttcacca ctgaagcccc gcagcccgtt tccgtcatcc gtggtcagcg
     4921 ggttcagcca gataccagct gacttgccgt tacggtcaaa agccggcagt gccacatacg
     4981 gctgcggata tttgcgcccg ggtgcaataa accgtgcagg actgtctccc ccggccagcc
     5041 ctgcctgacg gagaacggca cggccggcca ccacgtcccg cagctcccgc gccgtactga
     5101 acagccgctc tgcattcatg acctcccggt ccgatttcgg ctctagcaca tcgtgggcag
     5161 ttcctttctg tacggcattg ttaatggcat ccgtccagcc ctgacggtta tcggtataca
     5221 cctgcacatg ctgcttcata cgcgacaggg ccacgtaggc tgactcaaag ccggccatct
     5281 gtttccggtt accttccgtg ccttcaagcg caatggcaaa ggtttcactt gccccctgcg
     5341 caccgtgggc ggtgatggca taggccaggc caatatgttg ctctgcccgc tcctgaccgg
     5401 ggcgaatcac acgggtctgc tgtccgtccg acagcgtgac actgtcaccg gaaaccgctg
     5461 tcaccgtcca gacgctgttg gccacataac cgcgctcccg gtcactcttc gtgaagcgca
     5521 tccggtcacc ggttcccacc cggatggtgt ccggagtgta cagtgtgacg ccttcagcca
     5581 ccgcctcccg gggcgaaatc agccgcgtgt taccttccgc atcctgcagg gttatcagcc
     5641 cgtcatcctt gctgataccg gcaatccggt gatacacatt atccaccagg gcaagtgcgt
     5701 cccgatgtgt ctcccaggtg gagagacgac gcaactcccc gtcgcgtata ttcgctgtgt
     5761 tcaggacagg caccatgacc tgctcttttc ccagctcacc ggccttttcc cgtgcatcat
     5821 gaatcatgct gttcagtacg cgccggtcct cattcagatg cgtgacaatc agcgtctgct
     5881 cccgtgcttc cggcgtcctg ccggtataat cgcggacaat ggcttcatac agtgtcatgg
     5941 gaatatccgg aaaagcctca cctttcagca tcgccttctg ctgcgcttct gccagtttcg
     6001 cttcctggct gtgactgaac tccgtcacgg agtgctccgg tacccatgcg ccctcctgac
     6061 gtggcacctg agacggtttc acactctcaa gcccggacag tgccctttcc acatcccggt
     6121 taatcaggct gtataccgcc tcccgcagct ccggcgtctg gcgcacaatc tccttcatga
     6181 tggccacatc ggcagcactg cgcgtctgct ggagacggaa aggctgaccg ggcgcgatgg
     6241 cctgcaactg gtccgtgtca ccactggcca cggcacgacc gccaccggcc gcaatcaggg
     6301 cgtatgcccg tgccatgtcg gta
//
LOCUS       DAJOWB010000200         6241 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJOWB010000200 DAJOWB010000000
VERSION     DAJOWB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.200"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            71..1405
                     /gene="adeQ"
                     /locus_tag="OHO85_004550"
                     /gene_synonym="yicO"
     CDS             71..1405
                     /gene="adeQ"
                     /locus_tag="OHO85_004550"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCQ2673806.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1458..1910
                     /locus_tag="OHO85_004551"
     CDS             1458..1910
                     /locus_tag="OHO85_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCQ2673807.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2121..3311
                     /gene="nepI"
                     /locus_tag="OHO85_004552"
     CDS             2121..3311
                     /gene="nepI"
                     /locus_tag="OHO85_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCQ2673808.1"
                     /translation="MSEFIAENRGADAINRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3352..3645)
                     /locus_tag="OHO85_004553"
     CDS             complement(3352..3645)
                     /locus_tag="OHO85_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673809.1"
                     /translation="MKPTTLLLIFTFFAMPGIIYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3867..4685
                     /gene="nlpA"
                     /locus_tag="OHO85_004554"
     CDS             3867..4685
                     /gene="nlpA"
                     /locus_tag="OHO85_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCQ2673810.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4689..5612)
                     /locus_tag="OHO85_004555"
     CDS             complement(4689..5612)
                     /locus_tag="OHO85_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCQ2673811.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5723..>6241)
                     /locus_tag="OHO85_004556"
     CDS             complement(5723..>6241)
                     /locus_tag="OHO85_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2673812.1"
                     /translation="FVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLE
                     IPMMLIAGYYMKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILC
                     GIGMLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLG
                     IAMICLLFIKDI"
BASE COUNT         1510 a         1425 c         1583 g         1723 t
ORIGIN      
        1 tatcactgaa ccgttgttgt catttgtttt acaaaagcaa gggattaaat ctcctccaat
       61 ggacaaaaaa atgaataatg acaataccga ttacgtgagt aatgaatcag ggacgctttc
      121 gcgattattt aaactacctc agcatgggac caccgtccgc acagaattga ttgcggggat
      181 gaccactttt ttaaccatgg tgtacatcgt ttttgtgaac ccgcaaatcc tcggcgcggc
      241 acaaatggac ccgaaagtgg tgtttgttac cacctgtttg attgccggta tcggcagtat
      301 tgcgatgggg atatttgcta acttacccgt ggcgctggct ccggcaatgg ggctgaacgc
      361 cttctttgcc ttcgtggtcg tgggggcgat gggcatctcc tggcagaccg ggatgggcgc
      421 aatattctgg ggcgcagttg gactattttt gctcacgctg tttcgtatcc ggtactggat
      481 gatctccaac attcccttaa gtttacgtat tggtatcacc agcggaattg gattatttat
      541 cgccttaatg ggattaaaaa atactggcgt tattgtcgcc aataaagaca cgctggtgat
      601 gattggcgat ttaagttctc acggcgtgtt gttaggtatt ttagggtttt ttattataac
      661 cgtgttgtca tcacgtcatt ttcatgccgc ggtgctggtt tctattgtgg tgacgtcttg
      721 ctgtggatta tttttcggtg atgttcattt tagcggcgtc tattccattc cgcctgatat
      781 tagcggcgtc attggtgaag tagatttgag cggcgcgtta acacttgaac tcgccggtat
      841 cattttctcc tttatgctga tcaacctatt tgattcatca ggaacattaa ttggtgtaac
      901 tgataaagcg ggcttaatag atagtaacgg taaattcccc aatatgaata aggcgctgta
      961 tgttgatagc gtcagttcgg tggcgggggc gtttatcggc acctcgtctg ttactgccta
     1021 tattgaaagt acttctggtg tggcagtcgg tggtcgcacg gggctgactg cggttgtggt
     1081 tggcgttatg ttcctgttag ttatgttctt ctcaccgctg gtggcgatgg ttcctcctta
     1141 cgcaaccgcc ggagcgttaa tctttgttgg cgtgctgatg acttcgagcc tggcgcgcgt
     1201 taactgggat gattttaccg aatcggtgcc tgcgtttatt accacggtga tgatgccctt
     1261 tactttctcg atcaccgaag ggattgcact cggctttatg tcgtactgca tcatgaaagt
     1321 atgcaccggg cgctggcgcg atctgaacct gtgtgtggtg gtggtcgcag ctctgtttgc
     1381 actgaagatt attctggtgg attagtaact ggcgagggtt tccagatatc atgagttctg
     1441 attacgcagg agaactcatg atctggataa tgctcgccac gctggcggta gtgtttgtgg
     1501 ttggttttcg ggtgctgaca tccggggcca gaaaagcgat tcgccgtctc agcgatcggc
     1561 tgaacatcga tgtcgtaccc gtggagtcga tggtcgatca aatgggaaag tcagctggtg
     1621 acgaattttt acgttatttg catcgtccgg atgagtcgca cctgcaaaac gccgcgcagg
     1681 tgttgctcat ctggcaaatt gtcattgtcg atggtagcga acagaacctg ctgcaatggc
     1741 atcggatttt acaaaaagct cgcctcgccg cgccgattac cgacgctcag gtcaggctgg
     1801 cgctgggttt tctgcgcgaa accgaacctg aaatgcagga tattaatgct tttcagatgc
     1861 gctataacgc gttctttcag cctgccgagg gcgttcactg gttgcattga aaccgtttcg
     1921 tcttatcagc ctacagaccg cgcctgacct gtcgggtgga taagacgttc acgccgcatc
     1981 cggtaatctt tgccctgttc cgtttcgatc ttaactccag ctcaagcaac accgtaaaag
     2041 ttgcaaaaac gttaaatgcg tcacacattt caatgttaaa gtggtcggct tttcccctga
     2101 aacatgccac gggtaacacc atgagtgaat ttattgccga aaaccgcggc gcggatgcca
     2161 tcaaccgacc gaactggtca gccgttttct cggtggcgtt ttgtgtcgcc tgtctgatta
     2221 tcgttgagtt tttgcccgtc agtttgttga cgccaatggc ccaggattta ggcatttcgg
     2281 aaggggttgc cgggcaatcg gtgaccgtga ccgcctttgt ggcaatgttt gccagtttgt
     2341 ttattaccca gacaattcag gctactgacc gccgctacgt tgttattttg tttgccgttt
     2401 tgctgacgct ctcctgcttg ctggtttcct ttgctaactc attcagtttg cttttaatcg
     2461 gtcgtgcctg tctggggctg gcgctgggcg ggttctgggc gatgtcggcg tcgctgacca
     2521 tgcgtctggt gccgccgcgt acggtgccga aggcgctgtc ggtgatcttc ggcgcggttt
     2581 ctattgcgct ggtgattgcc gcgccgttgg gcagtttttt aggcgagctt atcggttggc
     2641 gcaatgtctt taatgcggcg gcggtgatgg gcgtgctgtg tattttctgg attatcaaat
     2701 cattgccttc actgccaggc gaaccctcgc atcagaaaca aaatactttc cgcttattac
     2761 aacgtccggg tgtgatggca gggatgatcg ccatcttcat gtctttcgcc gggcagtttg
     2821 ctttcttcac gtatattcgc ccggtgtata tgaacctggc gggattcggc gtggatggct
     2881 taacgctggt gctgttgagt tttggtatcg ccagctttat tggtacgtcg ctttcgtcgt
     2941 tcattcttaa acgttcggta aaactggcct tagcaggcgc gccgttaata ctggctgtga
     3001 gtgcgttggt actgacgttg tggggaagcg ataaaatcgt tgctaccggc gtggcgatta
     3061 tctgggggct aacttttgca ttggttcccg tcggctggtc aacgtggatc acccgctcgc
     3121 tggccgatca ggcagaaaaa gccgggtcta ttcaggtggc ggttattcag cttgctaata
     3181 cctgtggcgc ggcaatcggc ggttatgcgc tggataatat tggtctgact tcgccgttga
     3241 tgttgtccgg cacattgatg ttgctgactg cattgttggt tactgcaaag gtgaaaatga
     3301 agaaatcctg atatcaggta aagaagcgtt ggtcagtagt tgttgctgtt cttacatatc
     3361 cgggcattct accttaccca acgcttccca ataattgctc tgattattac gttccatcgc
     3421 cacgatggcc tcgcgtatat gttgtttatt gttttcatct gacaacatta acttttccca
     3481 tgcttcatct gataatgacg cctgtggcgt gcaaatttta cgtaaatctt gtaataccgt
     3541 ggttttctct tttgcggact gcagtgagct aaaaggcgac tcggcgtata tgatgcctgg
     3601 catagcgaaa aaagtgaaaa taagcagtag tgtcgttggt ttcatggatt caacctttta
     3661 caaatgtgta tcgaatgaag cataagtgta gtttgctttc atcgctctca tcatgctgta
     3721 cgaaacgcgc ttgcgtggtc agtaagaagt gccagacttt atattccact tttattcctt
     3781 tttattctta tcgatagcgt ttcgtttttt aaaccgcagc gaccttaccg ctatagtcag
     3841 gtaatcatta ataaaaggat aaaaaaatga aactgacaac acatcatcta cggacagggg
     3901 ccgcattatt gctggccgga attctgctgg caggttgcga ccagagtagc agcgatgcaa
     3961 aacacattaa agttggcgtt ataaatggcg cagaacaaga tgtcgcggaa gtcgctaaaa
     4021 aggtggcaaa agagaaatat ggcctcgatg ttgaactggt aggatttagc ggttcgttgc
     4081 taccgaacga tgcgacaaat catggcgaac ttgatgcgaa tgtcttccag catcgaccct
     4141 ttcttgaaca agataatcag gcgcatggtt ataaactggt agcggtggga aatacgtttg
     4201 tcttccctat ggcgggttat tccaaaaaaa tcaaaaccgt ggcgcaaata aaagaaggcg
     4261 cgacagtggc gatccccaac gacccgacca accttggtcg cgcactttta ctgttgcaaa
     4321 aagagaagct aattactctg aaagagggaa aagggttatt acctaccgcg ctggatataa
     4381 ccgataatcc tcgtcatttg cagattatgg aactcgaggg ggcgcagctc ccgcgcgtac
     4441 tggatgatcc caaagttgat gtagcgatta tcagcaccac ttacattcag cagacagggc
     4501 tttctccggt gcacgacagc gtatttattg aagataagaa ttcgccgtat gtgaatattt
     4561 tggtggcacg ggaagataat aagaatgcag aaaacgtgaa ggaatttctg caatcttatc
     4621 aatcacccga agtcgctaaa gcggcagaaa ccatttttaa cggtggcgcg gtgcctggct
     4681 ggtaattctc actcatgccg cgccggacga tttatttttc tggcgcggcg acgggaatcc
     4741 attgaaatca aaatcaccga tgacagaatc agcagcgttc ccagccagtc cggtaatgtg
     4801 aacgtgatcc ccagcaacag caaagagagt agcgcgctac ttaatggttc tgcacagctc
     4861 aaaatgctgg cttttggacc gccaattaat tgtgctcctt tcaggtacag actaaatgtc
     4921 agggacgtac caatgaccac caaataaaaa aacgccagaa tcaaactgcc gttaacgaca
     4981 aagtttgttc cttgtctggc ataaaaaggc aacagaatca gaccgccaat cagcatactc
     5041 cagccgacga ctggtaacgt gccatagcgg gcaattagcg ttgagggata ggtggtatag
     5101 aatgcagcag caaatgccga ggcaatgccc cagaacaacg cggcaggaga gatcgataat
     5161 gacgtcggat taccgtgtgt caccaataaa aaagtcccga ccagcgatgt caaaatagcg
     5221 cagaaaacca gaatgcccgg gcgcgattta cgcaccagtg agaaccaggc gacgataatc
     5281 gtcggtgaga ggaattgcag caccgttgcc gtggctgcgt tcgatttttc gatggttagc
     5341 aaaaaagtga gctgtacagt tagcgcgcca accacggaaa aaatcagcag gctaatggca
     5401 tctttatgat tgttaataat agaaaagatt ttatcgccat gaacaaatga cagcgtcagt
     5461 agaatcaaac cggcgaatat caaacgcgtc atagtcaaaa actgcgacga catctggctt
     5521 tgctccatga tgtattgcgc gcagacccct gaacttcccc acaacacggc ggcaatcaga
     5581 acgttcagca tcccctttct ggtggaaccc atttttccct ctgcatactg ttttgtcgtt
     5641 tatgtatgca ggcatgatag caaaaatggc gaggatggct ctgtttgcgc cacgccgatt
     5701 tttcttagcc cagactggtg agctaaatat ctttaataaa cagcaggcaa atcatcgcaa
     5761 tacccaacat ccctatcgcc agccagaaca acgcgtggta gctccagatt tcaaccataa
     5821 ttccgtcaac agagccggcg ataatccagc cgacgcgtga agtatttgca tataacgttg
     5881 tcgcagagcc tattttttca ggcatcaggt cctgaaaata aagcatgccg ataccacaga
     5941 gaataccaag gaagatggca tttagaattt gcaattccag ctcaactgcc ggagtcgtcg
     6001 ccatgagtac gctggcgtaa aaacacattc cactcacgat agcaatgagc attaatagcc
     6061 gcttgccaat acgtttcatg taatagcctg cgattaacat catcggaatt tccagaccgg
     6121 cagcgatacc aatcatttct ccagccagtt tatcggttag atgcagttca tcaataataa
     6181 atagcggcat atttatcatg tagagattat tcgccgccca catcattgaa cagaccacaa
     6241 a
//
LOCUS       DAJOWB010000201         6241 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJOWB010000201 DAJOWB010000000
VERSION     DAJOWB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.201"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1006
                     /locus_tag="OHO85_004557"
     CDS             <1..1006
                     /locus_tag="OHO85_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCQ2673813.1"
                     /translation="FIPGLALSAVITGVALWGGSIPAVAGAGFSALTLAILLGMVLGN
                     TIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGISGIIIDVLTLSSTFL
                     LACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEASKVTVAVATVVIFGT
                     VAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAISPDAENAAVISKMLR
                     VMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAIFNSFHLLPQSVVNML
                     VTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLIVGGGAINYVIQSVIA
                     "
     gene            1080..1937
                     /gene="nfo"
                     /locus_tag="OHO85_004558"
     CDS             1080..1937
                     /gene="nfo"
                     /locus_tag="OHO85_004558"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCQ2673814.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            1940..3028
                     /locus_tag="OHO85_004559"
     CDS             1940..3028
                     /locus_tag="OHO85_004559"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCQ2673815.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDET
                     VLAINDTHLLEQLSPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLAGEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3084..4334)
                     /locus_tag="OHO85_004560"
     CDS             complement(3084..4334)
                     /locus_tag="OHO85_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCQ2673816.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWTDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(4434..5375)
                     /gene="rihB"
                     /locus_tag="OHO85_004561"
     CDS             complement(4434..5375)
                     /gene="rihB"
                     /locus_tag="OHO85_004561"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCQ2673817.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            5505..6203
                     /gene="yeiL"
                     /locus_tag="OHO85_004562"
     CDS             5505..6203
                     /gene="yeiL"
                     /locus_tag="OHO85_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HCQ2673818.1"
                     /translation="MKEIHNNDLKLQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
BASE COUNT         1543 a         1594 c         1556 g         1548 t
ORIGIN      
        1 ttttattccg gggttagccc tgagtgcagt tatcaccggg gtcgccctgt ggggtggttc
       61 cattcccgcg gttgcgggtg ccgggtttag tgccctcacc ctcgcaatct tgttggggat
      121 ggttttaggc aacaccattt acccgcacat ctggaaaagc tgtgacggtg gtgtgctgtt
      181 tgccaagcaa tatttattac gtctgggtat cattctttat ggcttccgtc tgacgttctc
      241 gcaaattgcc gatgtcggta tcagtgggat catcattgac gtgctgacgc tgtccagtac
      301 cttcctgctg gcttgcttcc tggggcagaa agtgtttggt ctggataagc acaccagctg
      361 gttgatcggt gccggtagca gtatctgtgg tgctgccgcg gtactggcga ctgagccggt
      421 agtgaaagcg gaagccagta aagtaaccgt ggctgttgca accgttgtta tcttcgggac
      481 cgtcgcgatt ttcctctacc cggcgatata tccgctgatg tctcaatggt ttagtccgga
      541 aactttcggt atctacattg gttctactgt gcacgaagtg gcgcaggtgg tggcggcagg
      601 tcatgccatc agcccggatg cggaaaacgc agcagttatt tccaaaatgc tgcgcgtgat
      661 gatgctggct ccgttcctca tcctgctggc ggcgcgtgtt aaacagctgt ctggggcgaa
      721 cagcggcgag aaaagcaaaa tcactattcc gtggtttgcc atcttgttca tcgtagttgc
      781 catctttaac tcgttccacc tgttaccgca gagcgtggtg aacatgctgg taacgctgga
      841 taccttcctg ctggcaatgg cgatggcggc gctgggtctg accactcacg tcagcgcgct
      901 gaaaaaagcc ggggcgaaac ctctgctgat ggcactggta ctgtttgcct ggctgattgt
      961 tggtggtggt gcgattaact atgtgattca aagcgtcatc gcataaacca ctacatcttg
     1021 ctcctgttaa cccgctatca ttaccgtttt cctccagcgg gtttaacagg agtcctcgca
     1081 tgaaatacat tggagcgcac gttagtgctg ctggcggtct ggcaaatgcc gcaattcgcg
     1141 ccgccgaaat cgacgcaacc gcgtttgcct tgttcaccaa aaaccaacgt cagtggcgtg
     1201 ccgcaccgct cacgacgcaa accatcgatg aattcaaagc cgcctgtgaa aaatatcact
     1261 acacatcggc gcaaattctt ccccacgaca gttatctgat taacctcgga catccggtca
     1321 ctgaagctct ggaaaaatcg cgcgatgcct ttatagatga aatgcagcgt tgcgaacaac
     1381 tagggctttc tttgctcaac ttccaccctg gcagccatct gatgcagatt tcagaagagg
     1441 attgccttgc gcgtattgcc gaatccatca acattgcgct ggataaaact caaggtgtga
     1501 ccgcggtgat tgaaaacacc gccggtcagg gcagtaactt agggtttaaa ttcgaacatc
     1561 tcgcggcgat tatcgacggc gtggaagata aatcccgtgt cggcgtctgc attgatacct
     1621 gccatgcttt cgctgccggg tatgatttgc gtactccagc cgaatgcgag aaaacgttcg
     1681 ctgattttgc ccgtattgtc ggctttaagt atctgcgcgg gatgcacctt aacgatgcga
     1741 aaagcacctt tggcagccgc gttgaccgcc atcatagcct cggtgaaggc aatatcggtc
     1801 atgatgcgtt ccgctggatc atgcaggacg accgtttcga cggcattccg ctgatccttg
     1861 aaaccatcaa cccggatatc tgggcagaag agatcgcctg gctgaaagcg caacaaactg
     1921 aaaaagcggt agcctgaaga tgaataaccg ggaaaaggag atccttgcaa ttttacggcg
     1981 taacccgctg attcagcaga acgaaattgc ggacatgctg caaatcagtc gttcgcgcgt
     2041 tgcggcgcat attatggatt taatgcgcaa agggcggatt aaaggcaaag gttacattct
     2101 caccgagcag gaatactgcg tagtggtggg gacaatcaat atggatattc gcgggatggc
     2161 ggatatccat tacccgcaag cggcttctca tcccggtaca attcattgct cggcaggcgg
     2221 cgttggacgc aatatcgccc acaatctggc gctgttaggc cgcgacgtcc atttgctttc
     2281 agtgattggc gatgactttt atggcgaaat gctcctggaa gaaacgcgcc gcgccggcgt
     2341 gaatgtctcc ggctgcgttc gtttacatgg tcaaagcaca tcgacgtatc tggcaattgc
     2401 caatcgagac gatgaaaccg tgctggcgat taacgatacc catctgctgg aacagttatc
     2461 gccgcaatta ttgaacgggt cgcgcgattt acttcgtcat gcgggcgtgg tactggctga
     2521 ttgcaacctg acagccgagg cgctggaatg ggtctttacc ctcgctggtg agatcccggt
     2581 gtttgtcgat accgtttcag aattcaaagc gggcaaaatc aaacactggc tggcgcatat
     2641 tcacaccctg aaacccactt taccggagct ggaaatttta tggggacagg cgatcaccag
     2701 cgatgctgac cgtaatgctg cagtgaatgc gttgcatcag caaggtgttc agcaactgtt
     2761 tgtttatttg cccgatgagt cagtttattg cagcgaaaag gatggagaac aatttttgct
     2821 gaccgcacca gcgcatacga cagtagacag ttttggtgct gacgatggtt ttatggcggg
     2881 cctggtatat agctttctgg aaggaaacaa tttccgtgac agcgcccgtt ttgcgatggc
     2941 ctgcgcggca atttcacgcg ccagcggcag cttaaacaac cctaccctgt ctgccgataa
     3001 cgcgctttca ttagtgccaa tggtgtaaca atgttgccgg attcgacgct tgaccgcatc
     3061 ttatccggct cataaatcgc acactacgct aaaccaataa agaacccggc aatggtggca
     3121 ctcatcaggt tagaaagtgt cgccgccgcc agcgcgcgta aaccaagctg ggcgatttcc
     3181 ggcgcacggt gtggcgcaac cgcagaaaac gcccccacca ccaccccgat agaaccaaag
     3241 ttagcgaaac cgcacaacgc gaaggaaata atcgccacgg ttttagcatc cagagtgcca
     3301 gccgtttgca gatagggtga gaaattgaga taagcgacaa attcatttat tgccagtttc
     3361 tgtccaatca aactcccggc aagattcgca tcagtccagt caacacccat tacccacgcc
     3421 agtggggcca gcaggtaacc taaaatggac tccagcgagg catgttcaaa accaaaccag
     3481 ccgccaacgc cgccgataat accgttaatt aacgcaatga tggcaacaaa tgccatcacc
     3541 actgttgcca cacccgcagc gattttcagc ccggtcatcg cccctgtcgc ggcggcttca
     3601 ataatgcttt ttggcggtgt ttcggtgaaa gagaggttat taaaagaaac ctgcgaagat
     3661 tccgtagccg ggcttaacag gcgggcaaac aagatcccgc ctgggatcgc cattaacgat
     3721 gccgccagca aatattccac aggtacgccc agtgcggcgt aaccaatcat tgtcgaaccg
     3781 gcaattgagg ccatgccact acaaatcgct gtaaataatt cattgcgatt cagccgatcg
     3841 ataaagggtt tgacgattgc cggaatttcg ttttgcccga ggaaaatggt agtgaccgcg
     3901 acgaatgact cgatcttgct gatatttaat gctttctgga agataccgcc gagaatgcga
     3961 attaagatcc ccatcacacc gatgtaatag agaatactca ccagcgcggt gacgaagata
     4021 attgccggta atactctgaa accaaagata aatcctgcgc catcaaacag cgtgtccatt
     4081 ttcggcccga ccagcgaacc gaagataaat gcgctacccg cgtcgctgta cgccatcact
     4141 ttatgcacgc caaaagcgac tttttcagcg acccaacgcc ctggcggtaa ccaaagcata
     4201 atgccgccaa tcacaacctg taacactaac gccgcgccaa cggtacgcag gctgatcttc
     4261 ttcttgttta ctgacagcaa aaatgcaatt gccagcaata ccaccattcc cagaacactt
     4321 ctcatgacat ccatagtgat tatctcttca tgtcatattg ggcgacggct ttctgcccct
     4381 cagaaatagc caggcctgca tcttgcgata ccggcccggc ttgttatcaa cacttaatgg
     4441 gttttgatgt agccgcgcac gcactcttcg actaagcccc agaaccagtc ggtatcaata
     4501 gtgatgccga ctttggtatt agcgggcttg ccaagaacgc ccagctcgtc gcagacggta
     4561 cgcccataac aagggccact gttgacgtcc acttcgacgt acatctcctg ggttttaatg
     4621 ccatcagggt taatcagata accgatgcag gtggcgtcgt gcaccgggcc gccagccagg
     4681 ccgtagtttt cgaactgcgt tttgagagtg aagttcatga tgtcgctgaa cagctctccg
     4741 gcagggccgc ctgccctttc catccgagca attacgtccg gggtgcaaac ggtctggttg
     4801 gtaagatcga ggcccatcat cactaatgga acgccagagg tgaacaccac gcgtgcggct
     4861 tccgggtcag caaagatgtt gaactcggca gatggcgtga agttgcctgt accgtaagcg
     4921 ccgcccatta gcacaatttc acggattttg ggcaggatcg cgggttgcat acgcattgcc
     4981 accgcgatat ttgaaagcgg accaaccggc accagagtga tatcgccatc gcttgccatc
     5041 agggtatcaa tgatgtattt caccgcgtgg gtactttctg cctggcgggt cagcggctcg
     5101 aataccgggc catccagtcc ggtttcaccg tgaatattat cggcaacgat ttgttgacgc
     5161 ataatgggct gcggcatccc cgcataaacc ggaacattaa tctccagttt ctggcaaaca
     5221 ttcaggccat taattaatgt tttatcaagc gtctgattac ccgctacaat agtgatgcct
     5281 aataaatcta ttgccggatg tttcgctgcc atcatcattg caatagcatc atcatgaccc
     5341 ggatcacaat ccagaataat ttttctcttt tccattgttt atttcctctg tttccagttg
     5401 cgttattttt tctacagcaa agaaaattcg accgggccga tgattgaatc ttaacaacag
     5461 cgtacgtatg ctaaatatga gaaatctcat agcggataaa catcgtgaaa gaaatccaca
     5521 ataatgatct taagctgcaa ttgatgagtg aatccgcgtt taaggattgc tttttaacgg
     5581 atgtttcagc cgatacgcgg ctgtttcatt ttttagcgcg tgactacatt gtgcaggaag
     5641 ggcaacagcc gtcctggctg ttttacctga cgcgaggccg cgccaggctt tacgccacgc
     5701 tagctaatgg tcgcgtgtcg ctgatcgatt tctttgccgc cccctgtttt attggcgaga
     5761 ttgagttaat cgataaagac catgaaccac gtgcagtaca ggcgattgaa gagtgttggt
     5821 gccttgcgct ccctatgaaa cattaccgtc cgctgttatt aaacgacacg ctatttttac
     5881 gaaaactttg cgtcacctta agtcataaaa attatcgtaa tattgtttct ttaactcaga
     5941 atcaatcatt tccgttagtg aatcgcctgg cagcatttat attactctcg caagaaggtg
     6001 atctttatca cgaaaagcat acgcaagcgg cagagtattt aggcgtttct tatcgacatc
     6061 ttttatatgt tctcgcgcaa ttcattcacg acggtttatt aattaaaagc aagaaaggat
     6121 atctaattaa aaacagaaag cagttgtcag gactggcgct ggagatggac ccggagaata
     6181 aattctccgg gatgatgcag taaaaaaata atctggaata tactctaaat aattcgagtt
     6241 g
//
LOCUS       DAJOWB010000202         6203 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJOWB010000202 DAJOWB010000000
VERSION     DAJOWB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.202"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            219..845
                     /locus_tag="OHO85_004563"
     CDS             219..845
                     /locus_tag="OHO85_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCQ2673819.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            866..2968
                     /locus_tag="OHO85_004564"
     CDS             866..2968
                     /locus_tag="OHO85_004564"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCQ2673820.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            2981..3619
                     /locus_tag="OHO85_004565"
     CDS             2981..3619
                     /locus_tag="OHO85_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCQ2673821.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3683..4351
                     /locus_tag="OHO85_004566"
     CDS             3683..4351
                     /locus_tag="OHO85_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCQ2673822.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4348..5133
                     /locus_tag="OHO85_004567"
     CDS             4348..5133
                     /locus_tag="OHO85_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCQ2673823.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGGLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5137..5949
                     /locus_tag="OHO85_004568"
     CDS             5137..5949
                     /locus_tag="OHO85_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673824.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(5961..>6203)
                     /locus_tag="OHO85_004569"
     CDS             complement(5961..>6203)
                     /locus_tag="OHO85_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HCQ2673825.1"
                     /translation="FPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMH
                     DQPFVQGLTACAEIGEAMARAVVKPASRARRRLSFD"
BASE COUNT         1563 a         1393 c         1681 g         1566 t
ORIGIN      
        1 taattgataa cgatcaatgt taagggctca gaataatcac attatttttt ctcctaacca
       61 taaaggatta gtatatcggc gttaaatctt ttctcataca ctatcgggtt agggaaaata
      121 tctggtgttg gggacagagt cagcagcgta ttagttgaat gcttaatatc aggctgctga
      181 attgtcagca ctgtgtttgc gaagaggatg cgaaataaat gaacccggtt gatcgtccac
      241 tattagatat tggcttaacg cgattagaat tcttgcgtat atcaggaaaa ggccttgctg
      301 gtctaacaat tgcccctgcg ttactgtcac ttttgggttg caaacaggaa gatattgata
      361 gcggcacagt agggttgata aatacaccca aaggggtgct ggtgacccag cgagcgcgat
      421 gcacgggttg ccaccgctgt gaaatctctt gtaccaactt caacgatggc tcagtaggga
      481 cattcttctc ccgtatcaaa atccatcgca attatttctt tggcgacaac ggggttggct
      541 ctggcggcgg cctgtatggc gatctcaact ataccgcgga cacctgccgt caatgcaaag
      601 agccgcaatg catgaacgtc tgcccgattg gtgcgattac ctggcagcag aaagaaggct
      661 gtattaccgt cgatcataaa cgctgtattg gctgtagcgc ctgtactacg gcatgcccgt
      721 ggatgatggc caccgtaaat accgaaagta aaaaatcctc gaaatgtgtg ttatgcggtg
      781 aatgcgcaaa cgcctgccca acaggggcgt taaaaattat cgagtggaaa gatattactg
      841 tgtgaatctt gcaaaggaaa aaatcatggc taacggttgg acaggtaata tattaagagt
      901 caatctcacg acaggaaata ttaccctcga agattccagt aagtttaaaa gttttgtcgg
      961 tggcatgggc ttcggctaca aaattatgta tgacgaagta ccgccaggca cgaaaccttt
     1021 cgatgaagcg aataaattag tctttgctac cggcccatta actggatctg gtgccccctg
     1081 tagttctcgc gtaaatatca cctcactttc tacttttacc aaaggaaatt tagttgtcga
     1141 tgcccatatg ggtggctttt ttgcagcgca aatgaaattc gctggatacg acgtcattat
     1201 tatcgaaggg aaggcgaaat caccggtatg gctgaagatt aaagatgaca aagttagcct
     1261 ggaaaaagcc gatttcttat ggggaaaagg gacgcgcgca acgacggaag aaatttgtcg
     1321 attgaccagt ccggaaacct gtgtggcggc tattggtcag gctggggaaa accttgttcc
     1381 tctctctggc atgttgaata gccgtaacca cagcggcggt gcgggaactg gcgcaattat
     1441 gggttcgaaa aacctgaaag cgattgcggt tgaagggacg aaaggggtca acattgccga
     1501 tcgtcaggag atgaagcgtc tcaacgatta catgatgact gaacttattg gtgcgaataa
     1561 caaccatgtc gtgccaagta cgccgcaatc gtgggcagag tattcagatc ccaagtcacg
     1621 ttggacagca cgtaaagggc tgttttgggg cgcggctgaa ggtggtccga ttgaaacggg
     1681 ggaaattccg ccaggcaatc agaacacggt cggctttcgt acctataaat ccgtttttga
     1741 cttaggtccg gcggcagaga aatacacagt aaaaatgagt ggctgccact cttgcccgat
     1801 ccgttgtatg acccaaatga atattcctcg ggtgaaagag tttggcgtgc ccagcacagg
     1861 tggtaacact tgtgtagcca actttgtcca taccaccatc ttcccgaacg ggccgaaaga
     1921 ttttgaagat aaagacgatg gtcgtgtgat tggtaacctg gttggtctga atttgttcga
     1981 tgactacggc ctatggtgta actacgggca gctgcatcgc gactttactt attgttacag
     2041 caaaggggtg ttcaagcgtg ttctgccagc tgaagagtat gcggaaattc actgggatca
     2101 actggaagcg ggtgacgtta atttcattaa agatttttac taccgtctgg cgcatcgtgt
     2161 gggtgagctg agtcacctgg ctgatggttc atatgccatc gcagaacgct ggaatttggg
     2221 tgaagagtac tggggctacg caaaaaataa actctggtcg ccgtttggct atccggttca
     2281 ccatgccaat gaagcgtcag cgcaggtcgg ttccattgtt aactgtatgt tcaaccgtga
     2341 ctgcatgacg catacccata tcaattttat tggttcgggt ttgccattga aactgcagcg
     2401 tgaagtggcg aaagagcttt ttggttctga agatgcctac gatgaaacca aaaactacac
     2461 gccaatcaat gacgcaaaaa ttaaatatgc aaagtggtcg ctgttgcggg tctgtttgca
     2521 taacgccgtc accctgtgca actgggtctg gccaatgacc gtttcgccgc tgaaaagccg
     2581 taattaccgg ggcgatctgg cgcttgaagc caaattcttc aaagcgatca ccggcgaaga
     2641 gatgactcag gaaaaattag atttagctgc agagcgtatc tttacgttgc atcgtgccta
     2701 cacggtaaaa ctgatgcaaa ccaaagatat gcgtaatgaa cacgatctta tctgttcctg
     2761 ggtattcgac aaggatccgc agatcccggt ctttactgaa ggtactgaca aaatggatcg
     2821 tgacgatatg catgcttcgc tcacgatgtt ctacaaagaa atgggctggg acccacagct
     2881 tggttgtccg acccgcgaaa cattgcagcg tctggggctg gaagatattg ccgccgatct
     2941 ggcagcacac aatctactgc ctgtgtaagg aatgggtgag atgaaccatc gggatgaatt
     3001 gccgttagcg aaagtcagcg aagttgacga ggcgaagcgg caatggttac aggggatgcg
     3061 tcaccccgtt gatacggtaa ccgaaccaga accggctgaa atcctggcag agttcattcg
     3121 ccagcattct gcggctgggc agctggtggc gcgcgccgtg tttctctcgc caccctattc
     3181 ggttgcagaa gaggagctgt cagttttgct ggaaagcatt aagcagaacg gagactacgc
     3241 ggatatcgct tgtatgacgg gaagccagga tgattactat tactcaacgc aggccatgag
     3301 tgaaaactac gcggcgatgt ctttgcaagt tgtggagcag gacatttgtc gcgccattgc
     3361 acacgccgtt cgttttgagt gccagacgta tcctcgtcct tacaaagtcg ccatgctgat
     3421 gcaagcgcca tattacttcc aggaagcgca gattgaagcc gcgatcgccg cgatggatgt
     3481 tgcacctgaa tatgccgata tacggcaggt ggagtcatcg acggcggtgt tgtacttgtt
     3541 tagcgagcga tttatgacct atggcaaggc ttatggctta tgcgaatggt tcgaggtaga
     3601 gcagtttcag aacccctgaa atttcaggga gatgtcatgg atagggtgga cagttgcagc
     3661 cactgcatta ggggataatc agatgtcctt cactcgacgc aaatttgttc tggggatggg
     3721 aacagtaatc ttttttactg gttctgcgtc ttctctgtta gcgaacacga ggcaagaaaa
     3781 ggaagttcgg tatgccatga ttcatgacga gtcaaggtgc aatggctgta atatttgcgc
     3841 ccgcgcatgc cgtaaaacta accacgtccc cgctcaggga agccgtttat cgatagcaca
     3901 cattccagtc accgataacg acaacgaaac gcagtatcac ttttttcgcc agtcatgtca
     3961 gcactgcgaa gatgctccgt gcatcgacgt atgcccgaca ggggcgtcgt ggcgtgatga
     4021 gcaggggatc gtgcgggtag aaaaatcgca gtgcatcggc tgcagttatt gcattggcgc
     4081 ttgtccgtac caggttcgct accttaaccc tgtcactaaa gtggcagata aatgtgattt
     4141 ctgcgctgag tcccgactgg caaaaggctt tccgcccatt tgcgtcagcg cctgcccgga
     4201 acacgcattg atctttggtc gcgaagatag cccagaaatt caggcgtggt tgcagcaaaa
     4261 taaatactat caatatcagc tacctggtgc cgggaaaccg catctgtatc gccggttcgg
     4321 tcaacatttg attaaaaagg aaaatgtatg aacccgtcgc aacatgctga acagtttcag
     4381 agccagttag cgaactatgt gccacagttc actcccgaat tttggccggt gtggttgatc
     4441 attgccggag tattgctggt tgggatgtgg ttggtgctgg ggctgcatgc cttgcttcgt
     4501 gctcgtggcg tgaagaaatc agtcaccgat tatggtgaga agatttatct ttactgcaaa
     4561 gcggtcagat tatggcactg gtcgaatgcg ttactctttg tattgttgct ggcaagtggg
     4621 ctgataaatc actttgcgct ggtgggcgca actgcggtta aaagtctggt tgcggtgcat
     4681 gaagtttgcg gatttttgtt actcgcatgc tggctcggct ttgtgctgat caatgccgtt
     4741 gggggtaatg gtcaccacta tcgcattcgt cgtcaggggg ggctggagcg agcggcaaaa
     4801 caaacgcgat tttatttgtt tggcattatg cagggggaag aacatccttt cccggcaaca
     4861 acacagtcta aatttaatcc cttacagcag gtcgcctatg ttggtgttat gtatggattg
     4921 ctgccgttgt tactattgac ggggctgctg tgtctctatc cgcaagccgt gggagatgtg
     4981 tttcctggcg taagatactg gttattgcag gcgcattttg ctctggcatt tataagcctc
     5041 ttttttatct tcggtcatct ttatctttgc accacggggc gtacgccaca cgaaaccttt
     5101 aaaagcatgg tcgatggcta tcaccggcac taacgaatga tcattacacg agcagacctg
     5161 cgagaatggc gtatcggagc ggtgatgtat cgctggttcc tgcgccattt cccacgcggc
     5221 ggtagttatg ccgatattca tcatgcactg attgaggaag gatatacgga ctgggcggag
     5281 agtctggttg agtatgcgtg gaaaaaatgg ctggcggatg aaaacttcgc gcatcaggaa
     5341 gtttcctcta tgcaaaaact ggccacagac cccggagaga taccattttg cagtcagttt
     5401 gcccgcagcg acgatcatgc tcgcatcggc tgttgtgagg ataacgcccg aatcgcaacc
     5461 gctgggtatg ccgcgcaaat tgccagtatg ggttatagcg tgcggattgg aagtgtcggc
     5521 tttaacagtc acattggcag ctccggtgaa cgcgcacggg ttgctgtaac cggtaattct
     5581 tcgcggataa gcagcgctgg agatagtagt cggattgcga ataccggaat gcgagtacgc
     5641 gtttgtacat tgggtgaacg gtgtcatgtc gccagtaatg gtgacctggt gcaaatcgcc
     5701 agctttggcg caaacgccag aattgccaac agcggcgaca atgtacacat cattgccagc
     5761 ggtgaaaatt caacggtagt gagcacgggc gtggtcgact caattattct cgggccaggg
     5821 ggaagcgccg cactggcata tcatgacggc gaaagagttc gcttcgccgt cgccattgaa
     5881 ggtgaaaaca atattcgaac cggagtccgc tatcgtctta atgaacagca tcagtttgtt
     5941 gagtgctgat agctttacct ttaatcaaac gaaagacgcc gacgagcacg ggatgcaggc
     6001 tttacgaccg cccgagccat cgcctcacca atttctgcac atgccgtaag tccctgaacg
     6061 aaaggctggt cgtgcatcaa ccagggcaac gcgccgaacg ctacgcgtcc acgaatatct
     6121 tcgggttgct gtaacgtata atcttcgcca acatcaggga tttcatcccc tgttttctgt
     6181 aattgctcgc gtagcccagg gaa
//
LOCUS       DAJOWB010000203         6158 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJOWB010000203 DAJOWB010000000
VERSION     DAJOWB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.203"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..565
                     /locus_tag="OHO85_004570"
     CDS             <1..565
                     /locus_tag="OHO85_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCQ2673826.1"
                     /translation="GDMYGSRQKLWDMTWLYQEISDFSRIFNVEDRGQALIADFKKRE
                     ADLRQEFGKSKKDLSFVFWFSSASPSADAYVGGKNSASGFIASVLGGHNAITSETEWP
                     TVSWESIIAANPDVIVVASLDRNRWALDKAEEKIKFLKSDPAVSQLEAVKKGHIVVMD
                     GQAMNPTIRTLYGAEQVGEQLRKMGLN"
     gene            565..1602
                     /locus_tag="OHO85_004571"
     CDS             565..1602
                     /locus_tag="OHO85_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCQ2673827.1"
                     /translation="MSVGAIETSRSILLTGWCVLAAIVLALVIAVGVSVGELAIPLQN
                     VFYAISNRTGLTAEPLNRIYESVIWDFRLSRALVAACCGAGLAICGVVLQSLLKNALA
                     EPYVLGVSAGASTGAVSIVVLGLGSGAISLSAGAFAGAFAAFAFVALLTNGARGGNER
                     TILAGVAASQLFNAITAYTISTTASAQQARDVMFWLLGSFSGVRWPEFQLVIVVVLAG
                     LAVCLWYARALDAFTFGDDAAASLGIAVPRVRLILFTTAALITATIVSMAGSIGFVGL
                     VVPHVMRFFFGPLHRTLLIASALAGAILMVLADIASRLLIAPQSLPVGVVTALVGVPF
                     FAVIIYRSRNK"
     gene            1602..2363
                     /locus_tag="OHO85_004572"
     CDS             1602..2363
                     /locus_tag="OHO85_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2673828.1"
                     /translation="MSICAENITWKAGKKVIVNNVSLRVPRGETVGLLGPNGCGKSSL
                     LRVLAGLRRPDAGRVTLDGQDIARMAKKQLARRVACVEQHGMTEANMRVRDVVRLGRI
                     PHHSPFSNWSAQDDEAIAAALQRVAMLEKSEQGWLSLSGGERQRVHIARALAQSPSEI
                     LLDEPTNHLDIHHQMQLMQLISELPVTSIVAIHDLNHAAMFCDSLIVMQQGQILASGT
                     PEEILSEALLWDVFRVKTKIEISPYHGKKHIHFIV"
     gene            2420..3607
                     /locus_tag="OHO85_004573"
     CDS             2420..3607
                     /locus_tag="OHO85_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2673829.1"
                     /translation="MLLGSQFVFNIGFYAVVPFLALFLRDDMLLSGGLIGLILGLRTF
                     SQQGMFILGGTLADRYGAKAIILAGCVVRVAGFLLLACGASLWPIILGACLTGVGGAL
                     FSPSIEALLARAGTHSQANGKRSRAEWFALFAVCGELGAVIGPVAGGVLSGIGFRHIA
                     LAGAGIFLLALAVLFFGLPADGHTTTTRRRVPWWTPLRQPRFVAFILAYSSWLLSYNQ
                     LYLALPVEIQRSGGREQDLAPLFMLASLLIITLQLPLARFARRMGAVRILPVGFLLLS
                     ASFASVALFAAAPPAESWLRLMPAAGFVTLLTLGQMLLVPAAKDLIPLFAEESTLGAH
                     YGALATAGGCAVLAGNLLLGHLLDQALIPSPQAVYPWLLLALFPLCSAVALRAICRPL
                     AAT"
     gene            complement(3685..3942)
                     /locus_tag="OHO85_004574"
     CDS             complement(3685..3942)
                     /locus_tag="OHO85_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCQ2673830.1"
                     /translation="MEKKTISDYTEAEFLEFVRKICRAEGETEADDDKLVDEFRRLAE
                     HPDGSDLIYYPRDDREDSPEGIVKEVKEWRAKNGKPGFKQG"
     gene            complement(3943..4176)
                     /locus_tag="OHO85_004575"
     CDS             complement(3943..4176)
                     /locus_tag="OHO85_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCQ2673831.1"
                     /translation="MEVGKDPELLKQFKNQNKVLVTKGKSSFVPESERVGERERFELH
                     HIKRVTDGGAVYDIDNLRVVTPKHHIEIHRGNK"
     gene            4272..4543
                     /locus_tag="OHO85_004576"
                     /pseudo
     CDS             4272..4543
                     /locus_tag="OHO85_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4704..4883)
                     /locus_tag="OHO85_004577"
                     /pseudo
     CDS             complement(4704..4883)
                     /locus_tag="OHO85_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            complement(4929..5096)
                     /locus_tag="OHO85_004578"
                     /pseudo
     CDS             complement(4929..5096)
                     /locus_tag="OHO85_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5093..5923
                     /locus_tag="OHO85_004579"
                     /pseudo
     CDS             5093..5923
                     /locus_tag="OHO85_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc43 family transposase"
     gene            complement(5967..>6158)
                     /locus_tag="OHO85_004580"
     CDS             complement(5967..>6158)
                     /locus_tag="OHO85_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCQ2673832.1"
                     /translation="NGLYKAEVIHRKSWKNRTEVELATLTWVDWYNNRRLLERLGHTP
                     PAEAEKAYYASIGNDDLAA"
BASE COUNT         1214 a         1797 c         1737 g         1410 t
ORIGIN      
        1 cggcgatatg tacggcagcc ggcaaaagct gtgggatatg acctggctgt atcaggaaat
       61 tagcgatttt tcccgcattt tcaacgtcga agatcgtggt caggcgctga ttgccgattt
      121 caaaaaacgt gaagccgacc tgcgccagga gtttggtaaa agcaaaaagg atctctcctt
      181 cgtcttctgg ttctccagcg cctcaccttc ggccgatgcc tatgtcggcg gcaaaaacag
      241 cgcctccgga tttatcgcca gcgtgctggg cggacataat gcgattacct ccgaaaccga
      301 gtggcccacg gtgagctggg agagcattat tgccgccaac ccggatgtga tcgtggtcgc
      361 cagcctagat cgtaaccgct gggcgctgga taaagccgag gagaagatca aattcctcaa
      421 aagcgatccc gccgtcagcc agctggaggc ggtgaaaaaa ggccatatcg tggtcatgga
      481 cggtcaggcg atgaatccga ccattcgcac gctttacggc gcggagcagg tgggcgagca
      541 gctgagaaaa atggggctga actgatgagc gtcggcgcga ttgagacgag ccgcagcata
      601 ctgctcaccg gctggtgtgt actggcagcg atcgtgctgg cgctggtgat cgctgtgggc
      661 gtcagcgttg gcgagctggc catcccgctg cagaatgtct tttatgctat cagcaacaga
      721 accggcttaa ccgctgagcc gctcaaccgt atctatgaga gcgttatctg ggatttccgc
      781 ctcagccgcg cgctggtcgc cgcctgctgc ggcgccggcc tcgctatctg cggtgtcgtg
      841 ctgcaaagct tgctgaagaa tgccctcgcc gaaccctacg tcctcggcgt ctccgccggg
      901 gcctccaccg gcgcggtgtc gatcgtggtg ctgggccttg gctctggcgc aatttccctc
      961 tcggcaggcg cctttgccgg ggccttcgcc gccttcgcct ttgtcgcctt gctgactaac
     1021 ggcgcgcgcg gcggcaatga gcgtacgatt cttgcgggcg tcgccgcctc gcagttattt
     1081 aacgccatca cggcctatac catcagcacc accgccagcg cgcagcaggc gcgcgatgtg
     1141 atgttctggc tgctgggcag cttcagcggc gtccgctggc ccgaattcca gttggtcatc
     1201 gtggtggtgc tcgcgggtct ggcggtctgc ctgtggtatg cccgggcgct ggacgcgttc
     1261 accttcggcg acgatgccgc cgcctcgctg gggattgctg tgccgcgcgt gcgcctgatc
     1321 ctcttcacca ctgcggcgct gatcaccgcc accatcgtca gcatggccgg ctcaatcggc
     1381 tttgttggcc tggtggtgcc gcacgtgatg cgtttcttct ttggcccgct gcaccgaacg
     1441 ttgctgatcg ccagcgcgct ggcaggggcg atattgatgg tactggcgga cattgcgtcg
     1501 cgcctgctga ttgccccgca aagtctgccc gttggggtag taaccgccct ggttggggtg
     1561 cctttctttg ccgtgattat ctaccgctca aggaataagt gatgagtatt tgcgctgaaa
     1621 atatcacctg gaaggcaggc aaaaaggtca tcgtcaataa tgtctcgctg cgggtgccgc
     1681 gcggagaaac ggtcggactg ctggggccca acggctgcgg caaatcctcg ctgctgcgcg
     1741 ttctggcggg cctgcgccgc ccggatgcag gtcgcgtcac cctcgacggc caggatatcg
     1801 cccggatggc gaaaaagcag ctcgcccgcc gcgtggcttg cgtcgagcaa cacggcatga
     1861 ccgaggccaa tatgcgggtg cgcgacgtcg tgcgcctggg acgcattccc caccactctc
     1921 cgttctcaaa ctggagcgct caggatgacg aagcgattgc cgccgcgctg cagcgggtag
     1981 cgatgctgga gaaaagcgaa cagggatggt taagcctctc cggcggcgag cggcagcggg
     2041 tgcatatcgc ccgcgcgctg gcgcagagcc cgagcgaaat cctgctggat gagccgacca
     2101 accatctgga tatacaccat cagatgcagt taatgcagtt gatcagcgag ctgccggtaa
     2161 ccagcattgt ggccattcac gatcttaacc atgccgcgat gttctgcgat tcgctgatcg
     2221 tgatgcagca ggggcagatc ctcgccagcg ggacgccgga ggagattttg tccgaagcgc
     2281 tgctgtggga cgttttccgg gtgaaaacca aaatcgagat ctccccttac cacggcaaaa
     2341 agcacattca tttcatcgtt taggttgggc ggatatgcct cttttctccc tgcgccctgc
     2401 ggcaaccctc tggcctccgg tgttactggg aagccagttc gtttttaata ttggctttta
     2461 cgcggtcgta cctttcctcg ccctcttcct gcgcgacgac atgctgctct caggcggact
     2521 tatcggcctg atcctcgggc tgcgtacctt ctcccaacag gggatgttta tcctcggcgg
     2581 gacgctggcc gaccgctatg gcgctaaggc gatcattctc gccggctgcg tcgttcgcgt
     2641 cgccggtttt ctcctgctgg cgtgcggggc gtcgctgtgg cccattatcc ttggcgcctg
     2701 cctgaccggc gtcggcggcg ccctgttctc cccgtcgata gaggccctgc tggcgcgggc
     2761 cggtacccac agccaggcca acgggaaacg cagccgcgcc gagtggtttg cgctgtttgc
     2821 ggtatgcggt gaactgggag cggtgatcgg cccggtggcc ggaggcgtgc tgagcgggat
     2881 cggctttcga catatcgccc tcgccggcgc gggaatattt cttctggcgc tggcggtgct
     2941 ctttttcggt ctgcccgccg acgggcacac gacaacaacg cgcagacgag tcccatggtg
     3001 gacgcctttg cgccagccgc gctttgtcgc gtttattctc gcctacagct catggctgtt
     3061 gagctacaac caactctacc tcgctctgcc ggtggagatc caacgttccg gcggccgcga
     3121 gcaggatctg gcgccgctct ttatgctggc ctcgctgttg attatcaccc tccagctccc
     3181 tctcgcccgc ttcgcccggc gaatgggcgc cgtgcgcatc ctgccggtgg gctttctgct
     3241 gctgtcggct tcctttgcca gcgtggcgtt attcgccgcc gccccacccg ccgaaagttg
     3301 gctgcggtta atgccggcgg ctggttttgt cacgctgctc accctcggcc agatgctgtt
     3361 agtgcccgca gccaaggatc tgatcccgct gttcgccgag gagtcgacgc tgggcgccca
     3421 ctacggagcg ctcgccaccg caggagggtg cgcggtgtta gccgggaatt tactgctcgg
     3481 tcatttactt gaccaggcgc tcattccgtc gccgcaggcg gtttatccct ggcttctgct
     3541 ggcgctgttt ccgctgtgca gcgccgtcgc cctgcgggcg atctgtcgcc cgctggcggc
     3601 aacatgacgc agagcgggta acagcccgct ctggaagtga acagctggta tccatatttt
     3661 cagctaatcc tttaacaaag atcctcagcc ctgtttaaag cccggcttgc cgtttttggc
     3721 tcgccattcc ttcacttctt ttacgatacc ttcaggactg tcctcacggt catcccttgg
     3781 ataataaata agatcagaac catcagggtg ttcagctaat cgacgaaatt catccactaa
     3841 tttatcatca tcagcctcag tttcaccttc agcccggcag atttttctga cgaactccag
     3901 aaactccgcc tcggtatagt cagaaatcgt tttcttttcc atttacttat ttcctctgtg
     3961 aatttcaata tgatgcttgg gtgtcactac cctgagatta tcgatatcat aaacagcgcc
     4021 accatccgta acgcgcttga tatggtgcag ttcgaaacgt tctcgttcac ctacacgctc
     4081 tgattcagga acaaacgaag atttaccttt tgtaaccaga accttattct gatttttgaa
     4141 ctgcttcagt aactccggat ctttccccac ttccatccac aacgccttac ggaaccacgg
     4201 cagcctggag gctgcaaatc ggggtgtggc agaatatgag ctttcagagt aaccatccag
     4261 aatgagttcc catgtcacac aacctagagc accagaaagt ccacaccaga atggtgaaag
     4321 aggttctgaa agccgtcgcc agagccaata atcaccctta tcagtccgta tttacagact
     4381 ttatcgccgg tcatccctcc tgcacggtct gtttctggga aaccttcaac aaaatgtacc
     4441 cggattcacc gtacgaatac gtcacgttct gccatacctg ccgtcgtttg atttatacga
     4501 aacagaagcg gagatgaagg cggatgaccc gaagtggtgg tgattttcag ccccgaccgc
     4561 tgaaacgcct gttcacaatg tcgcggtata aaaatcgacc atgcagagag agaaaccacc
     4621 ggagtggtgg gaaaagcaga aaggctcacg tggcataagg cgtggacagc ggtatcgtct
     4681 ggtttttcaa tttcctatac gtgaacacaa tctgcaattc ggtatacagc gaacctgcca
     4741 ccataagtgt ttttatacca cttaactggc tcagcaccgg gggcaggctt cattctgaat
     4801 tcatcgcgca gacgtccttt cgagaaacac ctgtcttcgg caatctcctg cgcacgtctg
     4861 gtgaccagct catcattatt catggttctt gcatctccgt cagacgtttt tatatccgga
     4921 cagcccctct atggccactt ctgtgcagta tgcaatacac gcaggatata cacttttccc
     4981 cactggctgt cgacctcata aaccagaaca aaatgcgggt gaataaccag ttcccgggta
     5041 ccttccacac gaccattgcg gcctttatac ggctgcccgg ctaggctgct ggctgaacac
     5101 ccgcaaggtt cacactgatg acacaccagt aaaagtactg gcaccgggca ggaagaaggc
     5161 gaaaacagga tatatctgga cgtatgtccg ggatgaccgg aatgccggtt cgccagaacc
     5221 tccggcggtc tggttcgcct actcaccgga ccatcagggt aaacatccgg agcaacacct
     5281 tcgtcccttc cggggtatcc tgcaggcaga tgcgttcgca ggttacgatc ggctgttcag
     5341 tgccgaacgt gaaggcggcg cgttgacgga agcaggatgc tgggctcatg cgcggcgcaa
     5401 aatccacgat gtatatatca gtaccaaaag cgcgacggcg gaagaagcac tgaaactaat
     5461 cggcgaactg tacgccattg agcacgaaat acgcgggttg ccggtgtctg aacgcctggc
     5521 ggtcaggcaa atgcagagta aaccgctact gacttccttg tataagctga tgcaggagaa
     5581 agaacacacg ttatcgaaaa aatgccgtct gagagatgcg ttccggtata tcaggaagca
     5641 ctgggttgcg ttgtgcaact tctgtgatga cggtctggcg gaggcggaca ataacacagc
     5701 ggaaagagcg cttcgtgcag tctgtctcgg aaagaaaaat tacgtgttct tcggtagcga
     5761 tcacggcggc gagcgtggtg cactgctgta cgggctgatc ggcacctgcc gtctgaacgg
     5821 tatcgatccg gaagcgtatc tgcgccatat tctgagcgta ctgccggaat ggctttccaa
     5881 ccgagttgac gaactcctgc catggaacgt agtactcacc aatatgaacc gccccgggtt
     5941 tcctggagag tgttttatct gtgaactcag gctgccagat catcgtttcc gatggaagca
     6001 taataagctt tttctgcttc tgccggagga gtatggccca gcctttccag caatcgtcga
     6061 ttgttatacc agtccaccca cgtgagtgtg gccagctcca cttctgtccg gtttttccag
     6121 ctcttacggt gtattacctc cgctttgtaa aggccatt
//
LOCUS       DAJOWB010000204         6084 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJOWB010000204 DAJOWB010000000
VERSION     DAJOWB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.204"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            6..743
                     /locus_tag="OHO85_004581"
     CDS             6..743
                     /locus_tag="OHO85_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2673833.1"
                     /translation="MHDYSLRFANLALGYEGLPAIQNITGTIQKGSLTAIIGPNGSGK
                     STLLKGIAGILAPLSGSCTVEPKARIAYLPQISELDRTFPATVSDLVSLGLWPERGLF
                     RHHRIEDRKRLTDALGSVGLAGFEKRQLSALSGGQLQRALFARVILQQANIILLDEPF
                     NAIDATTIDDLLVLINRWHAQKRTVLAVMHDIGLVRNHFPQAILLNGKLVAWDETEQV
                     LRHTSLLPAQNMGTARALPGQHEATHS"
     gene            740..1597
                     /locus_tag="OHO85_004582"
     CDS             740..1597
                     /locus_tag="OHO85_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCQ2673834.1"
                     /translation="MNWLHEWVLAPFVGFGFMRRALYGSFLLCLSACPVGVFLTLRRM
                     SLVGDAMSHAILPGAAFGFLLYGLDILPMTIGGLVAGLIVAIFAGAVSRLTVQKEDAS
                     MAAFYLISLAVGVLIVSLRGSSVDLMHVLFGTVLALNHEALLLIFSVALLTLLLVSIF
                     WRALIAECLDPLFLRSVSRLGSPVHFIFLALVVLNLVAGFQALGTLLSVGVMVLPAVT
                     ARFWTRRVATMCIASVLIGMLACISGLLFSFHFSLPSGPSIILSVGIMYLVSAAAMMV
                     KNIHLKKLR"
     gene            1629..2579
                     /locus_tag="OHO85_004583"
     CDS             1629..2579
                     /locus_tag="OHO85_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCQ2673835.1"
                     /translation="MRKPGFISLCILTTSMLFLLFTFPAKAKINVIASFSVIGDMAKN
                     IGRDRIELRTIVGPNGDAHVYEPSPADAIAMSKADVILVNGLQLEGFISRLIEASETS
                     APVIETTKGANILQDPAGGHYHFYDGKAVFHAAPFDPHAWQSVGNSRIYVKNIAEAFC
                     AQDKSNCDFYQANVRAYDAKLADLENKIIKTVATIPQEKRTVVVGHNAFKYFEQAYGI
                     HFLSPQGISTESEASAADVAGILKKIKENNASAVFSENISNPRLVEQIAREAGLNVAG
                     VLYTDALSEPGGPASTYLEMMEHNVTAIATAASNSRNTQP"
     gene            2618..3868
                     /gene="aztD"
                     /locus_tag="OHO85_004584"
     CDS             2618..3868
                     /gene="aztD"
                     /locus_tag="OHO85_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallochaperone AztD"
                     /protein_id="HCQ2673836.1"
                     /translation="MKKRLLSTSISTLLLGLSVMPAFADEDVTAWRLFIADHDKPVVN
                     VIDALDGDKLATFNVKGPANLSRSESGATIFAIQGSAGVVSTIASGIAFHDHGDHADI
                     DIDAPKLLPLELTGKKPGHFVERQGKIAQWFDGEDSAQILGESAVLKGQKNITKVNVV
                     APHHGVAVPYDNYAVVSIPNPDDASKRPVGARVVDLQGKKVGDDALCPGLHGSAGSGD
                     TFALSCETGLLLITQKNAAPVIRHLPYAKTLPEGSTSTLIGGKGMQYFIGNYGPDRII
                     LVDPTESDSFRLIQLPTRRVHFVVDPVRAKFAYVFTEDGKLNQIDVLKGEISQSVRVT
                     DPYSMDGHWNDPRPRIAVADNKIYVTDPLKSKIIVLDATSFKKTSEISVEGQPFNIVA
                     VGGSGKVHGEHHDHEAHHHDDHAH"
     gene            complement(4092..4346)
                     /locus_tag="OHO85_004585"
     CDS             complement(4092..4346)
                     /locus_tag="OHO85_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004150562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673837.1"
                     /translation="MKISKIVAFSLTMASTPALAFQAKDTTGSDLSGKQLRATEDSII
                     SNFYSQHSRQFQTTTLGTCAAVAVPGCNCPFCSMLRNQKL"
     gene            complement(4710..5894)
                     /locus_tag="OHO85_004586"
     CDS             complement(4710..5894)
                     /locus_tag="OHO85_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCQ2673838.1"
                     /translation="MSVKKIAVIGAGVLGLSVARSLAQQGAKVTVFERSHVGAGTSST
                     TFAWINSNGKTPESYHHLNALAIDEHIRLQQERTTEGHWLKATGTYEWAAGAPEQKRL
                     QDRVSRLLELNYPVQNLSADELKRKVPEIRVGSHAGDIWYFPGECLLVPSVFMAWLVS
                     ELHVHNAELKTQSEVTELTEEKNRAEISLANGERWQGDSIVIAAGRWSAELASLLGQQ
                     LAMIDPNQPNKIACGFLGYTRPVLTQLQANLITPGLNVRPEGGGRLLLQCPDLDSFAN
                     PANPAPVDSYIGEEMHKRLRKLFNNMEMAQLERIAVGQRSRPADGLPGLGYISGKRRI
                     YLMVTHSGMTLGPLIGRLCAEEILHDKRSSLLSDFSPERLLGKTAKDFPAFTTLHFPA
                     AQ"
BASE COUNT         1549 a         1423 c         1355 g         1757 t
ORIGIN      
        1 atgtcatgca cgactactct ttgcgttttg caaatctggc gctgggttat gagggtttac
       61 ctgcgataca aaatattacc gggacgatac aaaaagggtc ccttacagcc atcatcggcc
      121 ctaacggttc tggtaagtca acactgctta aaggtatcgc agggattctg gctccgttaa
      181 gtggctcctg taccgtagaa ccaaaggcgc gcattgccta tctgccacaa atatcggaat
      241 tggatcgcac tttccctgca actgtttcag atttagtttc cctaggttta tggcctgaaa
      301 gagggttatt ccgtcatcac agaattgagg atcgtaaacg gcttactgat gcattgggat
      361 cagtaggatt agcagggttt gaaaaaagac agcttagtgc attatccggg gggcagcttc
      421 aacgcgcgct cttcgcacgg gtcattcttc aacaggcaaa catcatcttg cttgatgagc
      481 cttttaatgc cattgacgcc actactattg atgacttgct tgtgctgata aaccgctggc
      541 atgctcagaa acgtactgtc ttagctgtca tgcatgatat cggtctggtt agaaatcatt
      601 ttccacaggc cattttgtta aacggtaaat tagtggcatg ggacgaaaca gaacaagttc
      661 tgcgccatac ctcacttcta ccggcacaga atatgggcac agcacgtgct ttacctgggc
      721 aacatgaggc tacacattca tgaactggct tcatgaatgg gttctagcac cttttgtcgg
      781 cttcggtttt atgcgccggg cgctttatgg ttcatttctg ctttgtttaa gcgcttgccc
      841 ggttggcgtt tttttaacat tacgccgtat gagtctggtt ggtgatgcca tgtcacatgc
      901 tatcttgccc ggtgctgcat ttggcttctt actctacgga ttagatatat taccgatgac
      961 tattggtggg cttgtcgcag ggctgatagt cgctattttt gcaggggcgg tttcccgctt
     1021 aacagtgcaa aaagaagacg cttcaatggc tgctttttat ttaatctcac tcgcagtcgg
     1081 cgtgctcatt gtatcgcttc gaggctcaag cgtggatctt atgcatgtcc tatttggaac
     1141 tgtgcttgca ctcaatcacg aagcgctgtt gttaattttc tctgtcgcgc tgctcacact
     1201 tctgctggtt tctatattct ggcgcgcact tattgctgag tgccttgacc ctttatttct
     1261 tcgctccgtt tcacgattgg gatcgccagt ccattttatt ttcctggccc tggtggtact
     1321 gaatcttgtc gctggttttc aggcactcgg cactttgctc tctgtcgggg taatggtctt
     1381 gccagctgtg acggctcgtt tctggacgag acgtgtagca acaatgtgca tagcctctgt
     1441 cctgatagga atgcttgcat gtataagcgg tttgttattt tcatttcatt tttcactacc
     1501 ttcaggaccg tcaatcatat tatctgtggg aattatgtat cttgtctctg ctgctgccat
     1561 gatggtgaaa aacatccact taaaaaaact tcgttaatct tttcactaca aatcgaggaa
     1621 attatttaat gagaaaaccc ggttttatat ctttatgcat cctgacgaca tcaatgcttt
     1681 ttttactttt tactttcccg gcgaaagcga aaattaatgt catagcaagc ttttctgtta
     1741 ttggcgatat ggcaaaaaac atcggtcggg accgcatcga actgcgtact attgtcggcc
     1801 ctaatggcga tgcccatgtt tatgagccct ctcctgctga cgcgatcgct atgtctaaag
     1861 cggatgtcat actggtaaat ggtttacaac ttgaaggctt tatttcacgg ttgatcgagg
     1921 caagtgaaac ttccgcaccg gtaattgaaa caacaaaagg agcaaatatt ctccaggacc
     1981 cggcaggcgg acactatcat ttttatgacg gtaaagcggt ctttcatgct gcaccgtttg
     2041 atccacatgc ctggcaatca gtagggaatt ccagaatata tgtgaaaaat attgcagaag
     2101 ccttttgtgc acaagataaa agtaattgtg atttttatca ggctaatgtc cgtgcttatg
     2161 atgcaaaact tgcggatctg gaaaataaaa ttattaagac tgtcgctacg atcccacagg
     2221 aaaagcgtac tgtcgtcgta ggccataatg cattcaaata ttttgaacag gcatatggta
     2281 tacatttcct ttcccctcag ggtatatcca ccgaatcaga ggcctctgcc gctgacgttg
     2341 ccggcatact taagaaaatt aaagaaaata acgcatcagc tgttttttct gaaaatattt
     2401 ccaatccacg tctggttgaa caaattgcca gagaagctgg ccttaacgtg gctggcgtgc
     2461 tttatacaga tgccttgtca gaaccaggag gaccggcttc tacctacctg gaaatgatgg
     2521 aacataacgt aacggcgatt gcgacagcgg cgagcaatag ccgtaatacc cagccataaa
     2581 tacccgccga acagttttat atatgatgga gaacattatg aagaaacgct tattatctac
     2641 ttcaatctca acgctgcttt tgggcctttc tgtgatgcct gctttcgcag atgaagacgt
     2701 aaccgcatgg cgtctgttca tcgccgatca tgataaaccc gttgtgaatg ttattgatgc
     2761 actggatgga gataagctcg caacatttaa tgttaaaggc cctgcaaatt tatctcgcag
     2821 cgaaagtggt gccacaattt tcgctatcca gggtagtgct ggcgtagtat ctacaatcgc
     2881 ttcaggcatt gctttccatg atcacggaga tcatgcagac attgatattg acgctcccaa
     2941 attattaccg ctggaactaa cgggtaaaaa acctggtcat tttgttgaac gtcagggcaa
     3001 aattgcccag tggtttgacg gggaagatag cgcgcaaatc ctgggtgaaa gcgccgttct
     3061 taaaggtcag aagaatataa cgaaagtaaa tgttgttgcg ccacatcacg gcgttgctgt
     3121 tccttacgat aattatgctg tcgtatccat tcccaaccct gatgatgcgt caaaacgacc
     3181 tgtcggcgct cgcgtggtgg acctgcaagg taaaaaagtg ggtgatgatg ccttatgccc
     3241 cggacttcac ggctcagccg gctcgggtga tacttttgcc ctatcatgtg agactggcct
     3301 cctgctgatt acgcaaaaaa atgcggcacc ggttatcagg caccttccct acgcgaaaac
     3361 gttgccagag ggaagcacat ccaccttgat cggtggtaag ggaatgcaat attttatcgg
     3421 taattatggg ccagaccgga ttattctggt cgatcctacc gaatcggata gcttccgttt
     3481 gattcagttg cctacccgtc gagtccattt cgttgtagac ccggtacgcg cgaaattcgc
     3541 ctatgtgttt acagaagacg gaaagcttaa tcaaatagat gtcctgaaag gggaaatttc
     3601 gcagtcagtg cgtgtcactg atccctattc aatggatggc cactggaatg atccacggcc
     3661 gagaatcgca gtagctgaca ataaaattta cgtcaccgat ccgttgaaaa gtaaaattat
     3721 tgtgttagac gctacttctt ttaaaaagac cagtgaaatt agcgtcgaag ggcagccatt
     3781 taatattgtt gcagtcggcg gttcaggaaa agtacatggt gaacatcacg atcatgaagc
     3841 tcatcatcat gatgatcacg ctcattaata cttgtcaaac ctaacaacgc ctttcatagt
     3901 agatctgctg atagcagatc tactttctgg ctttaataat ctttttgaat ttaccctggg
     3961 aataacatcc atgaatatca aattcccttt catactattc acctcacttt acagcattta
     4021 ctgctctagt tgattacacc agaatctttt cagctttcag ctcttacaag taatatattt
     4081 atttaactct ctcaaagctt ttgattacgc aacattgaac agaaagggca attgcatcca
     4141 ggtactgcca cagcagcaca ggttcctaaa gtcgtcgtct gaaactgcct gctatgctgg
     4201 ctgtaaaagt tactgatgat gctgtcttct gttgcgcgca actgctttcc tgaaagatct
     4261 gaaccagtcg tatctttcgc ctgaaatgcc agagccggag tcgaagccat tgtcagggag
     4321 aatgcaacta tcttactgat tttcatgtta acctcttgtt aaatcttcct ctgaaactac
     4381 agtatgagaa aacgcataac aactagttat ctttcggtat gcggaatatg agcctggatg
     4441 ttataaaata acataacaca cccacaaaaa aatgatacta ttgggtagtg atgttcctct
     4501 acagaaggcg ttctggctga ctgtaaccaa tgcgtacgcg ggctataatg gccagatata
     4561 gcctgacatc acacaccttg tcgtattttt gcaagcttaa acgcttcagg cctgcccatg
     4621 ttggacaggc tttttgttaa tgccgctgaa caggatctag tttattagct ttctgatcct
     4681 catttcagtg gtgaacatcg actgaaaaat tactgtgcag ccgggaaatg taacgtagta
     4741 aaagcaggaa aatcctttgc cgttttccca agtaatcttt caggagagaa atcactcaaa
     4801 agcgaagaac gcttatcgtg gagaatctct tccgcgcaaa gccgtccgat caacggcccc
     4861 agagtcatac cactgtgcgt caccatgagg tagattcgtc gcttaccaga gatataacca
     4921 agccctggaa gaccatctgc cggtcgggaa cgctgcccaa cagctatacg ttccagctgt
     4981 gccatttcca tattattaaa tagcttccgt agccttttgt gcatttcttc accgatatag
     5041 ctatctaccg gtgccggatt tgcaggattg gcaaagctgt caaggtcggg acactgaagc
     5101 agtaatcgcc cccctccttc tggccggaca tttaaccctg gcgtgattaa attcgcctgt
     5161 aactgggtta acaccgggcg cgtatagccc agaaaaccac atgcgatttt attcggctgg
     5221 ttcggatcga tcattgccag ttgctgccca agtagcgaag cgagttcggc agaccatctt
     5281 cccgccgcaa tgactatact gtctccctgc caacgttcgc cattggcaag gcttatctct
     5341 gccctatttt tttcttcagt taactctgtc acttcgcttt gtgttttcag ttcggcgtta
     5401 tgaacatgaa gctcggacac cagccatgcc ataaaaacgg aaggtaccag taagcattca
     5461 cctggaaaat accagatatc tccggcatga gatcccacgc ggatttcagg aactttgcgc
     5521 ttaagttcat ctgcggaaag gttctgaaca ggatagttca gttccaaaag acggctgacc
     5581 cggtcttgta accgtttttg ctctggcgca ccagccgccc attcataagt cccagtcgct
     5641 tttagccaat gaccttctgt ggtccgttcc tgttgtagcc ggatatgctc gtcaatcgcc
     5701 agggcattga gatgatgata actttctggt gttttgccat ttgaattaat ccaggcaaaa
     5761 gtcgtgctgc ttgtccccgc ccccacatgg ctacgctcaa aaacagtgac ttttgctccc
     5821 tgttgcgcca gggagcgggc cacggacagg ccgagtacgc ctgcaccaat aactgctatt
     5881 tttttgaccg acatgccttt cctccttttt atcagcttcc tgacgttggg ctttcttatg
     5941 gcttatcgcc aagttcattc aatacgattt ccattgaagg ggctgatgta agcttatctt
     6001 tatattcagc cgatatgtca ggaaggctgg taaactgacc atcaagaaaa agaaaatcta
     6061 tcttattggc aatatcctcc agcg
//
LOCUS       DAJOWB010000205         5824 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJOWB010000205 DAJOWB010000000
VERSION     DAJOWB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.205"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            47..1198
                     /gene="yiaY"
                     /locus_tag="OHO85_004587"
     CDS             47..1198
                     /gene="yiaY"
                     /locus_tag="OHO85_004587"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCQ2673839.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTDKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1363..2901
                     /gene="aldB"
                     /locus_tag="OHO85_004588"
     CDS             1363..2901
                     /gene="aldB"
                     /locus_tag="OHO85_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCQ2673840.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3120..3263)
                     /locus_tag="OHO85_004589"
     CDS             complement(3120..3263)
                     /locus_tag="OHO85_004589"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673841.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            3446..3769
                     /locus_tag="OHO85_004590"
     CDS             3446..3769
                     /locus_tag="OHO85_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCQ2673842.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            3775..4911
                     /locus_tag="OHO85_004591"
     CDS             3775..4911
                     /locus_tag="OHO85_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCQ2673843.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(4908..>5824)
                     /locus_tag="OHO85_004592"
     CDS             complement(4908..>5824)
                     /locus_tag="OHO85_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2673844.1"
                     /translation="ENISHAATVLGIAQANVSKYLADFESKVGLKVFDRTTRQLMLTP
                     FGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYAPTGIITYLSKHVIDKIKDIG
                     DITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYASQRY
                     LEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNTQSA
                     LELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYQPWRVQYV
                     LDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         1434 a         1403 c         1527 g         1460 t
ORIGIN      
        1 tcctcagtaa gctgcccacc cttttttaca ctttcaggag tgtgttatgg catcttcaac
       61 tttctttatt ccttctgtga atgtcatcgg cgctgattca ttgactgatg caatgaatat
      121 gatggcagat tatggattta cccgtacctt aattgtcact gacagtatgt taacgaaatt
      181 aggtatggcg ggcgatgtgc aaaaagcact ggaagaacgc aatattttta gcgttattta
      241 tgatggcacc caacctaacc ccaccacgga aaacgtcgcc gcaggtttga aattacttaa
      301 agagaataat tgcgatagcg tgatctcctt aggcggtggt tctccacacg actgcgcaaa
      361 aggtattgcg ctggtggcag ccaatggcgg cgatattcgc gattacgaag gcgttgaccg
      421 ctctgcaaaa ccgcagctgc cgatgatcgc catcaatacc acggcgggta cggcctctga
      481 aatgacccgt ttctgcatca tcactgacga agcgcgtcat atcaaaatgg cgattgttga
      541 taagcacgtg actccgctgc tttctgtcaa tgactcctcg ctgatgatcg gtatgccgaa
      601 gtcactgacc gccgccactg gtatggacgc cttaacgcac gctatcgaag cgtatgtttc
      661 tattgccgcc acgccgatca ctgacgcttg tgcactgaaa gccgtgacca tgattgccga
      721 aaacctgccg ttagccgttg aagatggcag taatgcgaaa gcgcgtgaag caatggctta
      781 tgcccagttc ctcgccggta tggcgttcaa taatgcttct ctgggttatg ttcatgcgat
      841 ggcgcaccag ctgggcggtt tctacaacct gccacacggt gtatgtaacg ccgttttgct
      901 gccgcatgtt caggtattca acagcaaagt cgccgccgca cgtctgcgtg actgtgccgc
      961 tgcaatgggc gtgaacgtga cagataaaaa cgatgcggaa ggtgctgaag cctgcattaa
     1021 cgccatccgt gaactggcga agaaagtgga tatcccggca ggcctacgcg acctgaacgt
     1081 gaaagaagaa gatttcgcgg ttctggcgac taatgccctg aaagatgcct gtggttttac
     1141 taacccgatc caggcaactc acgaagaaat tgtggcgatt tatcgcgcag cgatgtaatc
     1201 atcatttcca caacggctgg caaattgtca gccgcttttt caactatctc tgtaaccctt
     1261 gcccgtaaat tcgtgatagc tgtcgtaaag ctgttaccga ctggcgaaga tttcgccagt
     1321 cacgtctacc cttgttatac ctcacaccgc aaggagacga tcatgaccaa taatccccct
     1381 tcagcacaga ttaagcccgg cgagtatggt ttccccctca agttaaaagc ccgctatgac
     1441 aactttattg gcggcgaatg ggtagcccct gccgacggcg agtattacca gaatctgacg
     1501 ccggtgaccg ggcagctgct gtgcgaagtg gcgtcttcgg gcaaacgaga catcgatctg
     1561 gcgctggatg ctgcgcacaa agtgaaagat aaatgggcgc acacctcggt gcaggatcgc
     1621 gcggcgattc tgtttaagat tgccgatcga atggaacaaa acctcgagct gttagcgaca
     1681 gctgaaacct gggataacgg caaacccatt cgcgaaacca gtgctgcgga tgtaccgctg
     1741 gcgattgacc atttccgcta tttcgcctcg tgtattcggg cgcaggaagg tgggatcagt
     1801 gaagttgata gcgaaaccgt ggcctatcat ttccatgaac cgttaggcgt ggtggggcag
     1861 attatcccgt ggaacttccc gctgctgatg gcgagctgga aaatggctcc cgcgctggcg
     1921 gcgggcaact gtgtggtgct gaaacccgca cgtcttaccc cgctttctgt actgctgcta
     1981 atggaaattg tcggtgattt actgccgccg ggtgtggtga acgtggtcaa tggcgcaggt
     2041 ggggaaattg gcgaatatct ggcgacctcg aaacgcatcg ccaaagtggc gtttaccggc
     2101 tcaacggaag tgggccaaca aattatgcaa tacgcaacgc aaaacattat tccggtgacg
     2161 ctggagttgg gcggtaagtc gccaaatatc ttctttgctg atgtgatgga tgaagaagat
     2221 gcctttttcg ataaagcgct ggaaggcttt gcactgtttg cctttaacca gggcgaagtt
     2281 tgcacctgtc cgagtcgtgc tttagtgcag gaatctatct acgaacgctt tatggaacgc
     2341 gccatccgcc gtgtcgaaag cattcgcagc ggtaacccgc tcgacagcgt gacgcaaatg
     2401 ggcgcgcagg tttctcacgg gcaactggaa accatcctca actacattga tatcggtaaa
     2461 aaagagggcg ctgacgtgct cacaggcggg cggcgcaagc tgctggaagg tgaactgaaa
     2521 gacggctact acctcgaacc gacgattctg tttggtcaga acaatatgcg ggtgttccag
     2581 gaggagattt ttggcccggt gctggcggtg accaccttca aaacgatgga agaagcgctg
     2641 gagctggcga acgatacgca atatggcctg ggcgcgggcg tctggagccg caacggtaat
     2701 ctggcctata agatggggcg cggcatacag gctgggcgcg tgtggaccaa ctgctatcac
     2761 gcttacccgg cacatgctgc gtttggtggc tacaaacaat caggtatcgg tcgcgaaacc
     2821 cacaagatga tgctggagca ttaccagcaa accaagtgcc tgctggtgag ctactcggat
     2881 aaaccgttgg ggctgttctg atataagaag ctggtcgcat tgggtattca ttgcctgatg
     2941 cgacgcttac gcgtcttatc atgcctacgg gaacctgacc gtaggccgga taaggcgtac
     3001 acgccgcatc cggcataaac aaagcgcacg ttgttacaat ttacgtttta aacctacgtg
     3061 cgcaataacc cagactacgc caacagaagt gcgtttctgg ggcgtgtttt gtcaggagtt
     3121 taatctcgcg tttcgatggt gagcgggcga accaggccgt aaatcaccat tggtgatccg
     3181 aggccaatca gcaataaacg gtagataatt gaaggtttaa tacgcttaat ctgtactgtg
     3241 gatgttttac tggagttatt cattagtttg gccaatagtt ccactatttc aatatgataa
     3301 acctcgtcct gttgaaaaac cagacagcac atttaagaaa gtatataaaa aacaatatgt
     3361 cgttatataa ataaggaaat tgcactgcat catggcattt cataatatgc gttaattgtt
     3421 cttttttata ttctttgagg gaaatatgtt cctagactat tttgcactgg gagtgcttat
     3481 ttttgtattt ctggtgatct tctatgggat cattatttta catgatattc cctacctgat
     3541 tgccaaaaaa cgtaatcatc ctcatgccga cgccattcat gttgctggtt gggtgagtct
     3601 ttttacgtta catgttatct ggccgttttt gtggatttgg gccacgcttt accgcccgga
     3661 gcggggatgg ggaatgcaaa gccatgattc atccgttatg caactgcaac agcgcattgc
     3721 cgggctggag aaacagctcg ccgacatcaa atcctcttct gccgagtaat acttatggat
     3781 ttactgatta ttttgaccta tgtggctttt gcatgggcaa tgtttaagat cttcaaaatt
     3841 cccgtaaata aatggaccat tcccacagcg gccctgggtg gaatatttat tgtcagcggt
     3901 ctaattctgt taatgaacta taaccatccg tataccttca aagcgcaaaa agcggttatt
     3961 tctattcctg ttgtcccaca ggtgacaggc gtggtgatcg aagtgacgga taagaaaaat
     4021 acgctgatta aaaaaggtga ggtgctattt cgactggacc cgacgcgtta tcaggcgcgg
     4081 gtggatcggc tgatggcgga tatcgttacc gcagaacata aacagcgggc gttgggcgca
     4141 gagttagatg agatggcggc gaatactcag caggcaaagg ccacgcggga taaattcgct
     4201 aaagagtatc agcgttacgc acgcggcagc caggcgaaag taaacccgtt ttcagaacgc
     4261 gatattgatg tggcgcggca aaattatctg gcgcaggaag cctccgtaaa gtcatcggcg
     4321 gcggaacaaa aacagatcca gagccagctg gatagcctgg tgttgggtga acattctcaa
     4381 atcgccagcc tgaaagcgca gctcgcggaa gcaaaatata accttgagca gacgatagtg
     4441 cgtgcgccga gcgatggtta tgtgacccag gtgctgattc gtccgggtac ctatgccgcg
     4501 tcgctgccgc tacgtccggt gatggtgttt atacccgatc agaaacgaca aatcgtggcg
     4561 cagttccgtc agaactcctt gctgcgcctg gctcccggcg atgatgcgga agtggtattt
     4621 aacgctctgc caggcaaagt gttcagcggt aagctggcag ccattagtcc agctgttccc
     4681 ggcggagctt atcagtcgac cggcacctta cagacgttaa acacagcgcc gggttcagat
     4741 ggcgttatcg cgaccattga actggatgag cacactgatt tgagcgcttt accagacggt
     4801 atttacgccc aggtggcggt ctactctgat catttcagcc atgtctcggt gatgcgcaaa
     4861 gtgctgttac gcatgaccag ttgggtgcat tacctttatc tcgatcatta atcttcgctt
     4921 tccgccgctt gttcaggccg cagttgctgc gactgagcaa gctggtggcg aatttcacgc
     4981 agcatttcat ccagcacata ctggacccgc cagggctggt attcccgttt acggaagata
     5041 gccaccagat ccagccacca ttcatacggg tgctgaaagc agggcaccag cgtgccgtct
     5101 tgcagatcgc tctgcacact tttgtctggc gcaaacacaa tccccaggtg atttcgtgcc
     5161 agctccagcg ccgattgcgt gttatcgcaa acgtaatttc ccttcacgcg ataatcccgc
     5221 acctctttac tcccggcgac gttgaagcgc cagatattcg cgtcatcaat catcatcgag
     5281 tcgatcaaaa tacaggagtg atgttcgagt tcgtcaggac ggctaatagg atgtttctca
     5341 agatagcgct ggctggcata agcggttacg gcatattggg taataaaact ggcaaccagc
     5401 gattcatctt ttggtggagc gtagctgatt aaaacatcac aatcatcagg aaattcgacg
     5461 ccttcataaa aggcattacg ctcaagattg caggttttta gcgataaggt gatatcaccg
     5521 atatctttaa ttttatcgat tacatgtttg gataaatagg taattatgcc ggttggggcg
     5581 tagatggtga cccgaccacg tttctcatgc ttataatctg caataaaatt attaagctgc
     5641 tcgtttctgt ccagcatgtc gttgatgtac ggtaacagcg cggtaccaaa aggtgtgagc
     5701 atcagctgcc gggtggttcg gtcaaagact tttaaaccca cttttgattc aaaatcagca
     5761 aggtatttgc tgacgttggc ctgtgcgatg ccaagtacag tcgcggcatg gctgatattt
     5821 tcac
//
LOCUS       DAJOWB010000206         5713 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJOWB010000206 DAJOWB010000000
VERSION     DAJOWB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.206"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(192..533)
                     /gene="trbJ"
                     /locus_tag="OHO85_004593"
     CDS             complement(192..533)
                     /gene="trbJ"
                     /locus_tag="OHO85_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HCQ2673845.1"
                     /translation="MPPVLWRGWIPFCRCTEGKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIHQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRRTGLNELS
                     EISEGTYNERK"
     gene            complement(463..1008)
                     /gene="trbB"
                     /locus_tag="OHO85_004594"
     CDS             complement(463..1008)
                     /gene="trbB"
                     /locus_tag="OHO85_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HCQ2673846.1"
                     /translation="MSLTKLLPVILLMMVTGVQASTRDEIERLWNPQGMAAQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYGGKKGAK"
     gene            complement(995..1279)
                     /gene="traQ"
                     /locus_tag="OHO85_004595"
     CDS             complement(995..1279)
                     /gene="traQ"
                     /locus_tag="OHO85_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HCQ2673847.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMAEQQEGGHVSH"
     gene            1360..1635
                     /locus_tag="OHO85_004596"
     CDS             1360..1635
                     /locus_tag="OHO85_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HCQ2673848.1"
                     /translation="MEKRSFKEKLEIIRNIIRESLLGNAAIIALIYAASHSLPVNAFP
                     DYLVISLLSIYIYFCELFRSHWIAVWFIIWSSVINLIILYGFYDRFI"
     gene            complement(1637..1984)
                     /gene="trbA"
                     /locus_tag="OHO85_004597"
     CDS             complement(1637..1984)
                     /gene="trbA"
                     /locus_tag="OHO85_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HCQ2673849.1"
                     /translation="MSEDYLKMFTGVVLLIFVIIAGYFFSERNDRKMFLLSSLVFLVI
                     NIACLYVLTASLWFLCGAIMSQGAALVVSIVAAALPDVTSFDRFRRIFICIMLSSVWS
                     GVMWFFIRGIMTG"
     gene            complement(2000..2743)
                     /gene="traF"
                     /locus_tag="OHO85_004598"
     CDS             complement(2000..2743)
                     /gene="traF"
                     /locus_tag="OHO85_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HCQ2673850.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSARKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(2736..2993)
                     /gene="trbE"
                     /locus_tag="OHO85_004599"
     CDS             complement(2736..2993)
                     /gene="trbE"
                     /locus_tag="OHO85_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HCQ2673851.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTDLQKPDERKSDERNEDE"
     gene            complement(3020..4828)
                     /gene="traN"
                     /locus_tag="OHO85_004600"
     CDS             complement(3020..4828)
                     /gene="traN"
                     /locus_tag="OHO85_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HCQ2673852.1"
                     /translation="MKRILPLILTLVAGMAQADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFKRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQSC
                     WAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGK
                     VMVCGGDVFCLDGECDKAQSGQSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQA
                     KFCKKAAAGYSNCCKDSGWGQDIGLAKCNSDEKALAKAKSNKLTVSVGEFCSKKVLGV
                     CLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFNRLDF
                     TNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(4825..5463)
                     /gene="trbC"
                     /locus_tag="OHO85_004601"
     CDS             complement(4825..5463)
                     /gene="trbC"
                     /locus_tag="OHO85_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HCQ2673853.1"
                     /translation="MKLSMKSLAALLMMLNGAVMASENVNTPESRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQPSQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RTVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(5472..>5713)
                     /locus_tag="OHO85_004602"
     CDS             complement(5472..>5713)
                     /locus_tag="OHO85_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraU family protein"
                     /protein_id="HCQ2673854.1"
                     /translation="RQGMIMETIGKNNAVCNEYPSPILPKERWRYQMVNMYPDSGQCH
                     PFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
BASE COUNT         1386 a         1545 c         1300 g         1482 t
ORIGIN      
        1 aaatcgaatt atttaattac gggtccgttt tccacgaatc aatacaggca tcaaaaataa
       61 tattcatagc tgtccataaa acaattccgg cagtcaggaa taccagagac aatccagcat
      121 acctgtcagt aattctggaa atgtatttat aatctcgttc tgtacttctg attttgagtt
      181 caggattatt tttattttct ctcattataa gttccctctg atatttccga taattcattc
      241 agaccagtcc tgcggctgta atatatttca gacaccgaaa tccatcccac aattgccccc
      301 acaaaaaaca caacagaaac agaaaaggta aggcacagcg aaacgacaat ttctacaggg
      361 ctggcgggat aaaaaagccc ctgatggata aaggggatat tcaggctgac gattattccc
      421 gtcgcgatgc cggatattcc ggcaatgagt aacaccacct gcttatttcg cacctttttt
      481 ccctccgtac atctgcagaa cggtatccat ccgcgccata aaaccggcgg catccgttgc
      541 cccctgtaaa agcggtaatg cctcaagcgt gttgacgttg accaggaagg tggtcggtgt
      601 ggccaccgga atattcggga agaaggtctg catcacgtcc ggtggcaccg gtaatgcttc
      661 cggaaaggcg gtatccccct gaccatccag ggtgtaggga aaaacggaaa agccgtactg
      721 ctgtgccagc tgcttcagta ccgggtcaaa ctggtgacag taagggcaat gcccctgcat
      781 aaacagcacc accttccagt cggccaggtt aacctgccta ccgttgctga ggcggaacca
      841 gcggggagcc ggcttttctg tcctggctga tgtgtctgct gccggttgtg caggctgcgc
      901 ggccatgccc tgcggattcc agagtcgttc aatttcatcc cgggtggagg cctgcacacc
      961 tgtgaccatc atcaacaaaa tgaccggcag taatttagtg agagacatgt ccgccctcct
     1021 gctgctctgc catcatggcc tgttgcctgg cttccagtgc ctcacgctct tcccggctga
     1081 cacgggcaat ccatgcatcc agcacctggt atgcgccgaa cagcacaagg atggccgcaa
     1141 tcagctccgc cagaaaaaaa gccatccagg gtttgctgta aacaagacgg gcgacgatat
     1201 gaaaccagac acccagggaa aatacccaca ttcccgtaat atcaagccgg ggtaaagaga
     1261 atctgcgttt acttatcatg ttaaactccg gttattattt acatgagagc attcatgtct
     1321 gtaatattcg cgtttgtgtg atattaatca gaggtacaaa tggaaaagcg atcttttaaa
     1381 gaaaaactgg aaataatcag aaacataata agagagtcgt tactgggaaa tgcggcaatt
     1441 attgcgctga tatatgcagc atcacattca ttaccggtca atgccttccc tgactatctt
     1501 gtaataagtt tattaagtat ctatatttat ttttgtgagc tattcagaag ccactggatt
     1561 gcggtatggt ttatcatatg gtcttcagta atcaacctca ttattttgta tggtttttat
     1621 gaccgtttta tatgacttag cctgtcataa tcccccttat aaaaaaccac atcactccgg
     1681 accataccga tgacaacata atacagataa atattcttct gaacctgtcg aagctcgtaa
     1741 cgtccggtaa tgcggccgca actattgaaa caaccagtgc tgcgccctga ctcataattg
     1801 caccacacag aaaccagaga ctggcggtaa gcacatataa acacgcgata ttaataacaa
     1861 ggaaaaccag tgagctcagg agaaacattt tcctgtcatt acgctcagaa aagaaataac
     1921 cggcaataat gacaaatatt aacagaacaa cacctgtaaa cattttcaaa taatcttcac
     1981 tcataaatca aatccgcgat taaaaattgg gtttaaaatc ttcagaaacg ttcaggaact
     2041 gttttgccag gtcgtcctgc gaaataaagc cgtatgataa cgggcgaaca ctgccctgtt
     2101 tcgggtcaac cagcatcatg gccgggaaat atttcacgcc gaggcgctgc gcctgcccct
     2161 ggtcagtccg ggaatccggc aacagcggat taatcacgcc atccacggaa acgggaataa
     2221 cactcagacc atacgtatcc cggaagccat taatgacctg cgccagttgc ccgtcgatgg
     2281 ggtcctgccc ccggtaaaaa aacatgatgc cgtagtgttc agccagtttc gcaatggcct
     2341 gtcgctgctg cgcctggtct gctgccagct ggttccgtac cgtgccgttg taatggctgt
     2401 actgcaggtt atagtccagt tcaggatgtg ccagcatggc ctttctggcg ctcatggtga
     2461 aaagcccggc ctgctgagtc cagtaatttt gcagccggaa atatttcaca aaattatcca
     2521 cgccgggata cagaatggct tcgtacagcg cccgctttgt tgccgtctgc agtgctgcca
     2581 gtttctgcat aatatccggc tcctgacggg gggctgcagg tacaggttta ttttcttttt
     2641 ccttcggatt tattttctcg ttataccact gccagcctgc atcttttcct gacgccggaa
     2701 aaataaagca gcagagtaac agtggcagta atgctttatt catcttcatt cctttcatca
     2761 ctttttcttt catcaggttt ctgcaggtca gtcagggcag aaaaacaaaa atacagaata
     2821 aacaggagca caccggagta aaggataacg aaagcagcgg caagcatccc gtctgccgtg
     2881 gtttctttta ccgtatccca cgaccagata attaccgggg aaatgacaat cgctgtaatc
     2941 aaaagccgga taagaaaatc aataaaacgg ttcgacgtga atatgacttt catcatttcc
     3001 cccggttatt tcccatacct tactgtcctg cctgtttcag ctggtcagca atctgctctt
     3061 tcactttctg cgtcagaacg ccactgtccg ggattttctg gttattcatc aggtcttcgt
     3121 agaagttagt gaaatccagt ctgttgaact gaattttctg cagctcatca accgtaatcc
     3181 cccgacagtc aggatgcttc gcactaccaa aactgatacg caactgaccg ttgcgcccct
     3241 gctgctggac tatctgcgcc agcttcgaat caaactgaca gtagctgcgt tttttctcca
     3301 gacagacacc cagcactttc ttcgaacaga actccccgac gctgacggtc agtttgtttg
     3361 attttgcttt agccagggcc ttttcatcac tgttgcattt ggccagcccg atatcctgtc
     3421 cccagccgct gtccttacag cagttgctgt atccggctgc agccttctta caaaatttcg
     3481 cctgaccggt gaaagcacgg acatccaccc cgttcagcgc cgcaacatcc ttacctgctg
     3541 cggccagtgc cgccagctga gaaacggctt cggcaaaatc attactctgc ccgctctgag
     3601 ccttatcgca ttcgccatcg agacagaaga cgtctccccc acagaccatc actttcccgg
     3661 atgtttttga ctcacaggag tacgtggcat attcatgcag acaggcacct tcttcggagt
     3721 aaaaggcaca ctggtgtgac accagggtac aggcaggatt gtcggtgtag gtctgacagg
     3781 ttcccttgtc tgctgactgc gtgacatacg tgtcccggta tgcccagcag ctctggtaca
     3841 tgctgtatga ctgaccgtct ttcaccagcg tcctgttccc gccagcctcc gtacattctg
     3901 tttttgtcag tttccccagc tccttgctga acgggcagct ttcagaccag acaacatacg
     3961 gggtgtacga ggcttttttt accctgacaa ccagacgcaa cttcattgcc gtatgacgtt
     4021 tgaattttgt attcccgacc cggacggggt gagatgacgt aaaagccaca ccggcagtca
     4081 gtgttttctg tactggtgta aatgaggcat ctgctgagta tttggcattc cagctcagtg
     4141 gggttcccag tactgtaatt gtcataggta cactcttctg caggtaagta cgactccagc
     4201 tgtaatgcgc atcgacaatt tcaccggtaa cgggagaggt aatggagaca acatactgac
     4261 cattcacttc acgtgcaggt agctgactca tttcatactc cagcgtccgg gtttcccatg
     4321 tggtactccc ctgcagctcc atacgggcag ttcgtgtgca gtactgctcc acctgtaaat
     4381 cccgctcaca ggtgtaattc gtgtattcac tcctgctaat ctcctgcgca ctgcactgct
     4441 gcccggtgtt tcccacaatg ctgtcagccc ggttcaccac atccctgccg gtctggataa
     4501 acggtgcatc cggtgaaaga atgtctttcg gcttgttcat gaatgactct gtaatggttt
     4561 ttccggtttc accggtcgcc cattccgtgg tgccgtcatt tttcaggccg ccatccccgc
     4621 cggcagtcac gccaccgtaa tattttgtct cgtccggatt cgcgttatag ccggggatac
     4681 tctcctgtgg cttaaagccc tgaatactgc tgcttccctg ccctttgatc tggtgagcaa
     4741 aatcagaacc agcccggtaa tcactgttgc tgtcagcctg tgccatgccg gcaaccagag
     4801 tcagtatcag aggtaaaata cgtttcattt cccggaatct cctttccctg ccagtaaatc
     4861 atgagccacc tgccggcagt cacccgtggc ggccactttc tccagcgcct ggccgacccg
     4921 caggtttccc cggatgatgt cgtatccctg actgcagaac accaccagtg ccggtacagt
     4981 gcgaatgccg tactgtgaaa acagcgtcgg gtcgatctga acaccatcag tcgcgccgtc
     5041 tttcaccagg gacaacacag cttcagcggt ggtcttcagg tcattgttca ccatgccccg
     5101 cagtgtggcc ggaataccgt agtgccgggt ttcgcccagc attcgtttca gcccctcttc
     5161 gggaatggaa aacgacacaa aatacagagc accctgccgg ggtttcccgt cctgcgaagg
     5221 ctgctgttta cgaaccagct catccaggaa atggttatct gaacgctgaa gggggttttc
     5281 cagcacctgt ttctccgccc aggctttcag ctgatggtca ggtttttcac gcagttgtct
     5341 gcttaaattt tcctgctgct tcaggaactg gcggctttca ggagtgttca cgttttctga
     5401 cgccataacc gccccgttca gcatcatcag cagtgctgcc agagatttca tactcagctt
     5461 catcactcac ctcacaggaa gacacagtta cgtttacgcc acatcaggta gccgaagttt
     5521 ttctttgtgt tgggcggatt tttcccggtt tcccagcggg tcacgctgcg cccgaacggg
     5581 tggcactgcc cgctgtccgg atacatattc accatctggt aacgccagcg ttctttgggc
     5641 aggattgggg acggatattc attacagacg gcgttatttt tcccgatggt ttccataatc
     5701 atgccctgac ggt
//
LOCUS       DAJOWB010000207         5687 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJOWB010000207 DAJOWB010000000
VERSION     DAJOWB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.207"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            190..900
                     /locus_tag="OHO85_004603"
     CDS             190..900
                     /locus_tag="OHO85_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCQ2673855.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            942..3593
                     /locus_tag="OHO85_004604"
                     /pseudo
     CDS             942..3593
                     /locus_tag="OHO85_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            3607..4137
                     /locus_tag="OHO85_004605"
     CDS             3607..4137
                     /locus_tag="OHO85_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2673856.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4150..4653
                     /locus_tag="OHO85_004606"
     CDS             4150..4653
                     /locus_tag="OHO85_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2673857.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            4713..5627
                     /locus_tag="OHO85_004607"
     CDS             4713..5627
                     /locus_tag="OHO85_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2673858.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         1639 a         1227 c         1262 g         1559 t
ORIGIN      
        1 agttaaactt actgttattt aaaatattta ctttgtagca aggttttcct attaaaagag
       61 aaccaacatt tattacaaac gcattaagtc tggaaacccg gcgactcgcc aatgtattag
      121 caacattaat cgattgtctg atgtaacggt tttcccgctt atcctcttaa cacatggaga
      181 atgcatcaga tgcaaactac gcgtaccccc tactctattt cctttatggc gacagtgtta
      241 cttttattgc tctttgcttg ccatagcacc gttgccaacg cagctgtcgc actgggtgcg
      301 acgcgggtaa tttatccggc aaaccagaaa caggttttat tgcctgtcac gaataatgat
      361 cctgccagtg tctatctcat tcagtcatgg atagaaaatg caggtgacca aaaagacacc
      421 cagtttgtca ttaccccacc gcttttttcc atgcaaggta aaaaagagaa tacgctgcgg
      481 ataatcaacg ctactaatca tcagttacca ggcgaccgtg aaagcctgtt ctgggtgaat
      541 gttaaagcta ttcctgctat ggaaaaagac cagaagaatg agaatacatt acagctggca
      601 attattagcc gcataaaaat gttttatcgc cccacgaacc tggcaatggc acctgaagaa
      661 gcgcctgcaa tgcttcggtt tcgtcgcagc ggtagcaaac tcacgcttat taatccaacg
      721 ccctatttca ttaccgtaac aaatatgaaa gcaggaaata gcaatctccc aaacaccatg
      781 gtgccgccaa aaggtgaagt atcggtggat atcacgcatg ctgcaaccgg ggatatcagt
      841 tttcagacga tcaatgatta cggtgcatta actccgcgta ttaaggcaac catgcagtaa
      901 gccagtgacc taattaataa taaactcaat cggaattaat aatgacagcc attcgtgccg
      961 catttaaggc atatcgtatg catcaggtgt taataatgcc tcgctttgca cggcttacca
     1021 ttgcccttgg cttagcaact gcggtatttc ccgttgacgc agagtattat tttaatccac
     1081 ggtttttaag caatgatctt gctgaatctg tcgatttatc cgcctttact aagggacgcg
     1141 aggcaccacc tggtacatat cgggtcgaca tttatttgaa cgatgaattt atgactagtc
     1201 gggatattac ttttattgca gatgataata atgcagaact catcccatgc ctgagtacag
     1261 atcttcttgt cagtcttggt ataaaaaaat cagcgttatt agacaataaa gaacattcgg
     1321 cagaaaaaca tgtgccggac aacagcgcat gcacaccact tcaggatcgt ttggccgacg
     1381 catcaactga gtttgatgtt ggtcagcaac atttgtcact ctccgttccg caaatttatg
     1441 ttggcagaat ggctcgcggc tatgtttctc ctgatctgtg ggaagaagga ataaatgctg
     1501 ggcttctaaa ctatagtttt aatggtaatt ctattaataa tcgtagtaac cataatgcag
     1561 gaaaatcaaa ctatgcatat ttgaatttac agagtggcat caacattggt agttggcgac
     1621 tacgcgataa ctcaacgtgg agttataaca gtgggagtag caattcatct gacagcaata
     1681 aatggcagca tatcaatacg tcggctgaac gtgacattat tcccttacgc tcacggttaa
     1741 cggtaggtga tagttatacc gatggcgata tttttgatag tgtgaacttt cgtggcctca
     1801 aaataaattc aacagaagcg atgttgcccg atagccaaca tggtttcgct cctgtgattc
     1861 atggtattgc ccgtggaacc gcacaagtga gtgtaaaaca gaacggatac gatgtttatc
     1921 agactactgt tccacccggc ccttttacta ttgatgatat caactctgcg accaatggtg
     1981 gtgatctgca agtaaccata aaagaggcag acggcagtat tcagacatta tatgttcctt
     2041 attcgtctgt tccggttctc caacgtgctg gatatacgcg ttatgcgctt gccatggggg
     2101 aatatcgtag tggaaataac ctgcaaagct ctcccaagtt catacaaggt agcttgatgc
     2161 atggtctgga aggaaactgg acaccttatg gcggaatgca aattgcagaa gattatcagg
     2221 ccttcaacct tggtattggg aaagatttag gactttttgg tgccttttct ttcgatatca
     2281 cgcaggccaa tacgacactt gcagatggca cccgtcacag tgggcaatcg gttaaatccg
     2341 tctacagcaa atccttctac cagacaggaa ccaatatcca ggtcgcagga tatcgctatt
     2401 ctacgcaagg tttttataac ttatccgaca gtgcctacag tcgaatgagt ggttacaccg
     2461 tcaagcctcc taccggagac accaatgagc agacacaatt tattgattat tttaatctgt
     2521 tctacagtaa gcgtggtcag gaacaaataa gcatctctta gcagcttgga aattacggta
     2581 cgacattttt cagtgccagt cgccaaagtt actggaacac gtcacgcagc gaccagcaaa
     2641 tatcatttgg attaaatgtg ccgtttggtg atattacgac ttcgctgaat tacagctatt
     2701 ccaataatat atggcaaaac gatcgggatc atttactcgc ttttacgctt aatgttccct
     2761 tcagtcattg gatgcgtaca gacagtcagt cggcatttcg taattcaaac gccagttaca
     2821 gtatgtcaaa cgatttgaaa ggcggcatga ccaatctatc gggggtttat ggcactctgc
     2881 tgccggataa taacctgaat tatagcgttc aggtcggtaa cacccacgga ggtaatacat
     2941 cgtctggcac cagtggttac agttctctta attatcgtgg agcttacggt aatacgaatg
     3001 tcggttacag tcggaatggt gacagcagcc agatttatta cggaatgagt ggtgggatta
     3061 ttgctcatgc tgatggcatc acctttggac agccgctggg cgacacaatg gttctggtta
     3121 aggctcctgg cgctgataat gtcaaaatag agaaccagac cggaattcat accgactggc
     3181 gtggctatgc aatattacca tttgcgacag aatatagaga aaatcgtgtc gctcttaacg
     3241 cgaattccct tgcagataat gttgaactgg atgaaaccgt agtcactgtc atcccaactc
     3301 acggtgctat tgccagagca acatttaatg cacaaatcgg cgggaaagta ttaatgacgt
     3361 tgaagtacgg taataaaagc gttccattcg gtgcaattgt cactcacgga gagaataaaa
     3421 atggcagcat tgtcgcggaa aatggtcagg tttatctgac tggacttcca cagtcaggga
     3481 aattacaggt ttcatggggc aatgataaaa actcaaactg tattgtcgat tacaagcttc
     3541 ctgaagtctc tcctggaacc ttgctgaacc agcagacagc aatctgtcgc taaggaacaa
     3601 tagattatga aatacaataa cgttattttc ctcggtttat gtctggggtt aaccacctat
     3661 tctgctttat ccgcagatag cgttattaaa attagcgggc gcgtcctcga ttatggctgc
     3721 acagtctcat cggattcgct taattttacc gtagatctcc aaaaaaacag tgccagacaa
     3781 tttccaacga ccggtagcac aagtccagcc gtcccttttc agattacgtt aagtgaatgc
     3841 agcaaaggga caacgggggt tcgggttgca tttaacggta ttgaggatgc agaaaataat
     3901 actctgttga aactggatga gggaagcaat acggcctccg gtttaggtat agaaatactg
     3961 gacggaaata tgcgtccggt gaaactgaat gaccttcatg ccgggatgca gtggatccca
     4021 ctggtaccag aacagaacaa tattttgcct tactccgctc gtctgaagtc aactcagaag
     4081 tccgtcaatc cgggactggt gagggcttcg gcaaccttta cccttgaatt tcaataatca
     4141 gagtcagata tgaaaagact tcacaagagg ttcctgttag ctacgttttg cgcgttattc
     4201 acagcaactc tccaggccgc cgatgtcact atcactgtta atggtcgggt agtcgctaaa
     4261 ccctgcacta ttcaaaccaa agaagctaac gttaatctcg gggatcttta tacgcgcaat
     4321 ctgcaacaac ctggttctgc atctggctgg cacaatatta ctttgtcatt aaccgattgt
     4381 ccggttgaaa caagtgcagt gacggcaatc gtgacaggtt caactgacaa tacgggttat
     4441 tacaaaaatg aaggtactgc cgaaaatatt cagatagagc tgagggatga ccaggatgcg
     4501 acgttaaaaa atggcgatag caaaacggtt attgttgatg agatcactcg taatgcacag
     4561 tttccactta aggcaagagc tatcacggtg aatggaaacg caagccaggg aacgatcgag
     4621 gcgctaatca atgtgatcta cacctggcaa taacgtcgat tcgattttct tagccttctt
     4681 tttaaatcaa ttttttaacc ttaggatata tcatgggtaa aacaatcagt ataaaggtcc
     4741 tgttcggtat ttatcttcta ttaatggcag gtaaagtttt tgccttcagt tgtaatgtcg
     4801 atggcgggtc aagtattgga gctggtacaa cctctgttta tgttaatctc gaccctgtaa
     4861 tacagccggg ccagaatctg gttgtagact tgtctcagca tataagttgc tggaatgatt
     4921 acggcggctg gtacgacact gatcatataa acctggtaca aggctcagct ttcgcaggct
     4981 ctctacagtc atataaagga tcgctgtact ggaataatgt gacctacccc ttcccgttga
     5041 cgacaaatac caacgtattg gatattggtg ataagacacc gatgccatta ccgttaaaac
     5101 tctatattac cccagtcggg gcggccggtg gagttgtcat aaaagccggg gaagttattg
     5161 cccgtattca tatgtataaa attgcaacat taggaagcgg aaatccgcgt aattttacgt
     5221 ggaatattat ttcaaataac agtgttgtta tgccaacagg cggctgcact gttgatagtc
     5281 gtaatgtcac cgtggatctc ccggactttc ctggaagcgc agaaataccc cttggcgtat
     5341 attgctcaag cgaacaaaag ctctcatttt atctttcggg tgccaccact gacagtgccc
     5401 gacaagtgtt tgctaacaca gcacctgatg caacaaaagc atcgggagtg ggtgtctctc
     5461 tgatgcgtaa tggaaaaata ctggctacgg gagaaaatgt ctccctggga acagtaaata
     5521 aaagcaaagt acctctgggt ttaagcgcaa cctatggaca aacaggaaac aaggtttcag
     5581 ccggaaccgt ccagtcagtc attggtgtca cttttatcta tgaataattg tgattttgat
     5641 cttagtctat gcgctccatt cacgaaaatt gcgcggataa ttatttt
//
LOCUS       DAJOWB010000208         5631 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJOWB010000208 DAJOWB010000000
VERSION     DAJOWB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.208"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..706
                     /locus_tag="OHO85_004608"
     CDS             <1..706
                     /locus_tag="OHO85_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCQ2673859.1"
                     /translation="KKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFS
                     LESVFILVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVA
                     GVLSAAFYVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVF
                     SSGILQYFLAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFI
                     VTVLSVYFLNRKYSEE"
     gene            complement(1909..2100)
                     /locus_tag="OHO85_004609"
     CDS             complement(1909..2100)
                     /locus_tag="OHO85_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCQ2673860.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENR"
     gene            2165..2488
                     /locus_tag="OHO85_004610"
     CDS             2165..2488
                     /locus_tag="OHO85_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCQ2673861.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     ARDDS"
     gene            2430..3188
                     /locus_tag="OHO85_004611"
                     /pseudo
     CDS             2430..3188
                     /locus_tag="OHO85_004611"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3166..3696
                     /locus_tag="OHO85_004612"
     CDS             3166..3696
                     /locus_tag="OHO85_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCQ2673862.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSN
                     ESLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFAT
                     TGSLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETN
                     WTKDNGKLNAVRLVQE"
     gene            3703..3936
                     /locus_tag="OHO85_004613"
     CDS             3703..3936
                     /locus_tag="OHO85_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCQ2673863.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDN
                     AMLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
     gene            4417..5469
                     /locus_tag="OHO85_004614"
     CDS             4417..5469
                     /locus_tag="OHO85_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673864.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLR
                     KWVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARR
                     GEVSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKD
                     GLKIYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIEKGFYKDQEQALA
                     DIRNMTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPG
                     ELTKDERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSF
                     QPRKFENKPLYEKEDD"
     gene            5471..>5631
                     /locus_tag="OHO85_004615"
     CDS             5471..>5631
                     /locus_tag="OHO85_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCQ2673865.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVI
                     LSWIVLFIK"
BASE COUNT         1486 a         1154 c         1514 g         1477 t
ORIGIN      
        1 aaaaaaactc ccattaaact caaaagcgat acttgcttat gcgacaggcg tttttgaacc
       61 attcttagcg tatactttta cactctacgg actttatttt atttcagcag gaagcgcatc
      121 aattattttt tcacttgaat cagtgtttat attggtgttg tctgttctta tttgctcagt
      181 taaaattaat tccccgcaat attttatatt gttccttata ggtgcaattg ttggcagtat
      241 tttggtagcc ttgccagaca tcagtaatgg atcgaataat attgttggat attgtcttgt
      301 tgttgccggt gtgttatcgg cagcatttta tgttgttata tcgtctcgcc ttattaaatc
      361 gtttgagcct atcacattat tgagcggaca gttgacattc tcccttatac tgtcatcgct
      421 ctttattttt atatctaaat catcaattta tcttccgttg gaatccgtga ttattgtttt
      481 ttcatctgga atattacaat attttttagc tttttgtttt tacttgaact ccttgcgttg
      541 gctcccagtt catttggcag ggataatgtt gtactttatt ccgctagttg cattattttt
      601 gtcatggttg tttttggatg aacaattcac attcttacag ggggtaggat ttattgtaac
      661 tgtgctaagt gtatattttc ttaacagaaa atactcagag gagtaacatt ctgttcagga
      721 ttgccccccg gcctccatcg ataccgtgcc aaatgcacgt aaaaaacggg agcgtttctg
      781 gtgtcggaat gtaaccaagg gggtcagagg cgcgctaaaa cgctctcagg ggagtcaaat
      841 agagacgttt gtaacaaaaa gatcaaagga tcctcttgag atcctttttt tctgcgcgta
      901 atctgctgcc tgtaaacgaa aaaaccgcct tgcagggcgg tttgatcgaa ggttaagtca
      961 gcttgcagac tgcttaacca ggtaactgtc ttatgcgaga cgagtcacca aaattgttct
     1021 tctagtgtag ccgcactccg gcagccactt caagtactct ctaaatatct cgcacatgac
     1081 ttttaccaat ggctgctgcc agtggcgctt tgtggtgtct tttccgggtt ggacccaaga
     1141 gaacagttac cggagaaggc gcagcagtcg ggctgaacgg ggggttcctg catacagccc
     1201 agcccggagc gaactgccta agtcgccccg agataccaga agacgtgagc tatgagaaag
     1261 cgccacgtgt cccgtaaggg agaaaggcgg aaaaggtatc cggtaatcga tatggcggga
     1321 acgtgggggc gcaggaggga gcgattcacc ggaaacggtg atgatcttta agccacgtcg
     1381 ggtttcgcca ccgctggcgg atttcatggt tgagccgcaa ggctcccaca gatgcaccga
     1441 aaaagtgtct gttttgtgaa gtccgtcagg ggggcggagc ctatggaaaa agcgcctccg
     1501 gcgcggcctt tcccggggtt tcgttttttg cgcacatgtt ctttcctggt ttatccccgg
     1561 gttctgtgga taacagggtt agcgtttttg agaaaggaga gtccgctcgc cgcagccgaa
     1621 cgaccgagcg tagcgagtca gtgagcgagg aagcggaaga gcgcctgaac tggcattttc
     1681 tccttacgca tctgtgcggc atttcacacc ggacatggcg cacttttcat acaatccgca
     1741 ctgatgccga atggttaagc cagtatacac tccgctatcg ctacgtgact gggtcagggc
     1801 ttcgccccga caccccaaag gtcaacggcc tgacgcgccc tggcgggctt gtctgctccc
     1861 ggcatccgct cacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agcggttttc
     1921 accgtcatcc ccgaagcgtg cgaggcaact gcggtaaagc tcatcggcgt ggtcgtgaag
     1981 cgattcacag atgtctgcct gttcatccgc gtccagttcg ttgagcttct ccagcagcgt
     2041 taatgtctgg cctctgatga agcgggccat gttaagggcg gttttttcct gtttggtcac
     2101 tgatgcctcc gtgtaagggg gggttctgtt catgggggta atgataccga tgaaacgcga
     2161 gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgctgtga
     2221 gggtaaacaa ctggcggtat ggatgcggcg ggtctgcctg ggtgagccgg ttgcccgttc
     2281 cgggaagctg ccgacactgg caccgccgtt actgcgtcag ctggccgcca tcgggaataa
     2341 ccttaaccag acagcgagga aggtgaacag cgggcagtgg tcttccggtg accgggttca
     2401 ggtggtggcc gcactgatgg ccatcgggga tgagctgcgc cggctgcgtc tggcggtcag
     2461 ggaacagggg gcgcgggatg atagttaagt ttcatgccag gggaaaaggt ggtggcagtg
     2521 gtccggttga ttacctgctg ggaagggagc gtaaccggga aggggcaacg gtgcttcagg
     2581 ggaatccgga agaggtccgg gaactcatcg atgccacgcc atttgcgaag aaatacacgt
     2641 caggtgttct gtcgttcgcg gagaaggagc tgccgcctgg agggcgtgaa aaagtgatgg
     2701 cgagctttga gcgtgtactg atgcccggtc tcgagaaaaa tcagtacagc atcctgtggg
     2761 tggagcacca ggacaaggga cggcttgagc tgaattttgt cattccgaac atggagctac
     2821 agaccggaaa acgcctccag ccgtactacg accgggcaga caggccgaga attgatgcct
     2881 ggcagacgct ggtgaatcac cattacggtc tgcatgaccc caacgccccg gagaaccgcc
     2941 ggacgctgac ccttcctgat aacctgccgg aaacgaaaca ggcgcttgct gagggcgtca
     3001 cacgcgggat agacgcgctt taccatgccg gagagataaa aggccgtcag gatgtgattc
     3061 aggcgctcac agaggcgggg cttgaagtgg tcagggtgac gcgaagcagt atcagcattg
     3121 cagacccgaa cggcgggagg aatatcagac tgaaaggagc gttttatgag caatctttta
     3181 cagacgggcg cggagttcga gaaaaagctg aaagagagag ccgaatctac agagaaaatg
     3241 ctgaacgacg agttcaggaa gctcgaagaa tctgtaaaca gggaactgac ctcaaacgaa
     3301 tctttaatca ggaacgctat caacgaccat acaacagccc tgaaagaact gctggagagg
     3361 tatcagaaaa cgaccgtcga tacgatggac gcgcactgga aaaccgtact gaaaatgagc
     3421 gtgaaacgct ggctgtggct gattatcgtc tcagtactga tgtttgccac gaccgggagt
     3481 ctgctgtggt accagggcat gaagataaac gcgaatatga atattctccg cgagcagaaa
     3541 gagagtctgg agaagctgaa cgcgaagaca tggggcgtgc gttatcacga ggacagcaac
     3601 ggacgttttc tggtgttgcc gaaggggatg aaagccgaaa cgaactggac gaaggacaac
     3661 ggcaaactga acgcagtgag actggtgcag gagtgacaga gcatgacgga gctggaaaga
     3721 catttgctga acgcattaga gcaactgcaa caggactata tgcagcggct gaacgaatgg
     3781 gagaacgcct tcgtggaatt gcagaaaatg ttttcgctta cgcaacggga caacgcgatg
     3841 ctgaacgggc gagtcatgca gttgagtcag caggtgcagc acttgagcga gcggacagaa
     3901 cgcttgagcc agttatacag cgagaacagg agataaggga tgaacggctg atgcaggaac
     3961 gcgaacatga gttatctctg gagcgggagc gtcagccgga aatccaggag cggacactgg
     4021 atggcccttc gctgggatgg tgaaaccatg aaaaatgacg tgttcagtgg attaaggcgg
     4081 gctaatgtgg cctgtagcat ctggttgcat aggtattcat gcggttaaaa tttatcaggt
     4141 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     4201 gcaccgaacg cgttttggcg gtgcgtacaa tgcgacgtta tggtaaatgg agcggatttt
     4261 caggaaggag tgatactaac aactcgaaca ctgtgcaaat aatgattttt ttaaaatatc
     4321 ggtaacgata ataaattatt tcgttttacg ccggtaataa atataatgta gtttcagtat
     4381 ataaaaaaat atatgaggtg tcttgtggga gctttcatga atcttcaaca acgtataaat
     4441 aaactaccac aattatcatc cagtttttca tttggaaaag atattgataa tattcattca
     4501 tttatcttta acgaaacttc taaagataaa attgaagatt tactaagaaa atgggtgtca
     4561 ggcaatcagc cctgtgtatt tggtaaattg gcaagcaaga aaattaaagg gcttgatttc
     4621 catttatcta ttgtaaatag cccccaacta tataatgatg atggtcattt gtttgatttt
     4681 ttgcgtaatg aaagagttcg ttttaaagaa cgcgcaagaa gaggagaggt cagtgcgcat
     4741 ctcatttatt ttattcatcc acaacttgcc tttgctcgtc catcagaaga gttagtggac
     4801 atacaaaaat atatttgttc gctgcatatg ccagagtgct atccaataaa agaggatgtt
     4861 atttatactg aaagcgtacc atttcaggat aaagatgggc taaaaatata taaggctggt
     4921 gttaatgttt tttactcttc tgctcataga acaagaaatc atgatagaag aataccaggg
     4981 ggaatactta tttctgtaaa tgccccgggg cattttatga ggctggcgat agaaaaaggt
     5041 ttctataaag atcaagaaca agcgttagcg gatattcgta acatgactat tcagtctgtt
     5101 ggtaagggtg ggtattcaca tccggaaggg atttctacaa catggcattc agaatctaat
     5161 ttggatcgtt ttggatgccc agtgcacacc ggtaatagtt cttattacag cggtttttat
     5221 catactgatg tattaattcc aggagaatta actaaggatg aaagattgct ccatgaaata
     5281 gaaaatagtg atccaatgat cttcaactgg aatgtattgt tttatgtttc attagaagag
     5341 tttcctatag atgatcctta ttatggagag tttattggta ttcctgtaga tgatgcatct
     5401 atgtttttta actcctttca accaaggaaa tttgaaaaca aaccccttta tgagaaagag
     5461 gatgattaat atgattaaag gaatgctttt tgctttttta gcagcgttct catggggggc
     5521 agctatcgta atgagcaaaa aaggagttga agaactagat gccggagggg tgtttttctg
     5581 gcagatttgt agtgcagtaa tactatcttg gatagttctt tttataaaaa g
//
LOCUS       DAJOWB010000209         5525 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJOWB010000209 DAJOWB010000000
VERSION     DAJOWB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.209"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            274..2559
                     /gene="nrdA"
                     /locus_tag="OHO85_004616"
     CDS             274..2559
                     /gene="nrdA"
                     /locus_tag="OHO85_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCQ2673866.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKHGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDDSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2794..3924
                     /gene="nrdB"
                     /locus_tag="OHO85_004617"
     CDS             2794..3924
                     /gene="nrdB"
                     /locus_tag="OHO85_004617"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCQ2673867.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            3924..4178
                     /gene="yfaE"
                     /locus_tag="OHO85_004618"
     CDS             3924..4178
                     /gene="yfaE"
                     /locus_tag="OHO85_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCQ2673868.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4232..4882)
                     /gene="inaA"
                     /locus_tag="OHO85_004619"
     CDS             complement(4232..4882)
                     /gene="inaA"
                     /locus_tag="OHO85_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCQ2673869.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5061..5303
                     /locus_tag="OHO85_004620"
                     /pseudo
     CDS             5061..5303
                     /locus_tag="OHO85_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1378 a         1455 c         1356 g         1336 t
ORIGIN      
        1 gttcaagaaa tcgccgaaca gttattttta acaaattttt ctcttcccat tgactttccc
       61 ggacaccttg tctgacctaa ggtgcgcgaa agccactttt tccttcctga gttatccaca
      121 aagttatgca cttgcaagag ggtcattttc acactatctt gcagtgaatc ccaaacatac
      181 cccctatata tagtgttcta agcagcttcc cgtactacag gtagtctgca tgaaactatt
      241 gcggaaagaa ttccaaaaac aggtacgaca tacatgaatc agaatctgct ggtgacaaag
      301 cgcgacggta gcacagagcg catcaatctc gacaaaatcc atcgcgttct ggattgggcg
      361 gcagaaggac tgcataacgt ttcgatttcc caggtcgaac tgcgctccca cattcagttt
      421 tatgacggta tcaagacctc tgacatccac gaaaccatta tcaaggctgc cgcagacctg
      481 atctcccgtg atgcgccgga ttatcagtat ctcgccgcgc gcctggcaat cttccacctg
      541 cgtaaaaaag cctacggcca gtttgagccg cctgcgctgt acgaccatgt ggtgaaaatg
      601 gtcgagatgg gcaaatacga taatcatctg ctggaagact acacggaaga agagttcaag
      661 cagatggaca cgtttatcga tcacgaccgt gatatgacct tctcttatgc tgccgttaag
      721 cagctggaag gcaaatatct ggtacaaaac cgcgtaaccg gcgaaatcta tgagagcgcc
      781 cagttccttt atattcttgt tgccgcgtgc ctgttctcga actacccgcg tgaaacgcgc
      841 ctgcaatacg tgaagcgttt ttacgacgcg gtttccacat ttaaaatttc gctgccgacg
      901 ccaatcatgt ccggcgtgcg taccccgact cgtcagttca gctcctgcgt actgatcgag
      961 tgcggtgaca gcctggattc catcaacgcc acctccagcg cgattgtgaa atatgtttcc
     1021 cagcgtgccg ggatcggcat caacgccgga cgtattcgtg cgctgggcag cccgattcgc
     1081 ggtggcgaag cgttccatac cggctgtatc ccgttctaca aacatttcca gacagcggtg
     1141 aaatcctgct ctcagggcgg tgtgcgcggc ggtgcggcaa cgctgttcta cccgatgtgg
     1201 catctggaag tggaaagcct gctggtgttg aaaaacaacc gtggggttga aggcaaccgc
     1261 gtgcgtcata tggactacgg ggtacaaatc aacaaactga tgtatacccg tctgctgaaa
     1321 ggtgaagata tcaccctgtt cagcccgtcc gacgtaccgg ggctgtacga cgcgttcttc
     1381 gccgatcagg aagagtttga acgtctgtat accaaatatg agaaagacga cagcatccgc
     1441 aagcagcgtg tgaaagccgt tgagctgttc tcgctgatga tgcaggaacg tgcgtctacc
     1501 ggtcgtatct atattcagaa cgttgaccac tgtaataccc atagcccgtt tgatccggcc
     1561 atcgcgccag ttcgccagtc taacctgtgc ctggaaattg ccctgccgac caaaccgctg
     1621 aacgacgtca acgacgagaa cggtgaaatc gcgctgtgta cgctgtctgc tttcaacctg
     1681 ggcgcaatta atagcctgga tgaactggaa gagctggcaa ttctggcggt tcgtgcactt
     1741 gacgcgctgc tggattatca ggattacccg atcccggccg ccaaacatgg agcgatgggt
     1801 cgtcgtacgc tgggtattgg tgtgatcaac ttcgcttact acctggcgaa gcacggtaaa
     1861 cgctactccg acgacagcgc caacaacctg acgcataaaa ccttcgaagc cattcagtat
     1921 tacctgctga aagcctctaa tgagctggcg aaagagcaag gcgcgtgccc gtggtttaac
     1981 gaaaccactt acgcgaaagg gatcctgccg atcgatacct ataagaaaga tctggatacc
     2041 atcgctaatg agccgctgca ttacgactgg gaagctctgc gtgagtcaat caaaacgcac
     2101 ggcctgcgta actccacgct ttctgctctg atgccgtccg agacttcttc gcagatctct
     2161 aacgccacta acggtattga accgccgcgc ggctacgtca gcatcaaagc gtcgaaagac
     2221 ggtattctgc gccaggtggt gccggactac gagcatctgc acgacgccta tgagctgctg
     2281 tgggaaatgc cgggtaacga tggctacctg caactggtgg gtatcatgca gaaatttatc
     2341 gatcagtcga tctctgctaa caccaactac gatccgtcac gcttcccgtc aggaaaagtg
     2401 ccgatgcagc agttgctgaa agacctgctc accgcctaca aattcggggt caaaacgctg
     2461 tattatcaga acacccgtga cggcgctgaa gacgcgcagg acgatctggt gccgtcaatc
     2521 caggacgatg gctgcgaaag cggcgcatgt aagatctgat attgagatgc cggatgcggc
     2581 gtaaacgcct tatccggcct acggctcgga tttgtaggcc tgataagacg cgccagcgtc
     2641 gcatcaggct ccgggtgccg gatgcagcgt gaacgcctta tccggcctac ggctcggatt
     2701 tgtaggcctg ataagacgcg tcagcgtcgc atcaggcaca ggatgcggcg taaaatgcct
     2761 tatccggcat taaactccca acaggacaca ctcatggcat ataccacctt ttcacagacg
     2821 aaaaatgatc agctcaaaga accgatgttc tttggtcagc cggtcaacgt ggctcgctac
     2881 gatcagcaaa aatatgacat cttcgaaaag ctgatcgaaa agcagctctc tttcttctgg
     2941 cgtccggaag aagttgacgt ttctcgcgac cgtatcgatt accaggcact gcctgaacac
     3001 gaaaaacaca tctttatcag caacctgaaa tatcagacac tgctggattc cattcagggc
     3061 cgtagcccga acgtggcact gctgccgctg atctcgatcc cggaactgga gacctgggtc
     3121 gaaacctggg cgttttcaga aacgattcat tcccgttcct atactcatat cattcgtaat
     3181 atcgttaacg atccgtctgt tgtgtttgac gatatcgtca ccaacgagca gatccagaaa
     3241 cgtgcggaag ggatctccag ctattacgat gagctgatcg aaatgaccag ctactggcat
     3301 ctgctgggcg aaggtaccca caccgttaac ggtaaaactg tgaccgttag cctgcgcgag
     3361 ctgaagaaaa aactgtatct ctgcctgatg agcgttaacg cgctggaagc gattcgtttc
     3421 tacgtcagct ttgcttgttc cttcgcattt gcagaacgcg aattgatgga aggcaacgcc
     3481 aaaattattc gcctgattgc ccgcgacgaa gccctgcacc tgaccggcac ccagcatatg
     3541 ctgaatctgc tgcgcagcgg cgcggacgat cctgagatgg cggaaattgc cgaagagtgt
     3601 aagcaggagt gctatgacct gtttgttcag gcagctcaac aggagaaaga ctgggcggat
     3661 tatctgttcc gcgacggttc gatgattggt ctgaataaag acattctctg ccagtacgtt
     3721 gaatacatca ccaatatccg tatgcaggca gtcggtttgg atctgccgtt ccagacgcgc
     3781 tccaacccga tcccatggat caacacctgg ctggtgtctg ataacgtgca ggttgctccg
     3841 caggaagtgg aagtcagttc ttatctggtc gggcagattg acgcggaagt ggacaccgac
     3901 gatttgagta acttccaact ctgatggccc gcgttaccct gcgcatcact ggcacacaac
     3961 tgctgtgcca ggatgaacac ccttcccttc tggcggcgct ggaatcccac aatgtggcgg
     4021 ttgagtatca gtgtcgcgaa ggttactgcg gctcctgtcg cacacgcctg gttgcaggtc
     4081 aagttgactg gattgccgaa ccgttagcct ttattcagcc gggggaaatt ttgccctgtt
     4141 gttgccgggc aaaaggcgat attgaaatcg agatgtgaat tggttgtagt gccagataca
     4201 acgcttatgc gtcttatctg gcctacaacg attacatggc gtagtaatac gctttcacct
     4261 gctcccagtc cgctttgggg attggctcca gatatttttc cagctggcgg aagtcatgat
     4321 taatcgcttt atcgcgacgc aagcggcgac gacttttctc caggtcaaga aaaccagctt
     4381 ctgccttacc ttctgttttc acatagatat ggcgaacata acaacagcca tgctgacgat
     4441 taatgctatg cattttctta aacgccagcg ccactgcttt caacatggct tgccgtactt
     4501 cgtcagaata aggcgatact gcatgctggg cataccagtc agcaatgctg atgaaccccg
     4561 ccatatcttc agtcaccagc aacgctcgcc attcaccctc aattttcacc gcttcaccaa
     4621 aaacgatctt cggcacgatg acaccggccc gttccagttc tttaattacc gcaacctcac
     4681 ggacaatcgt tggtcggccg aacggataac gtacggaatg aaacagatga tgcgtcatgc
     4741 gctttacata cagctttttg ccgttgcgct cgacgcattg caccccgctc ataccattac
     4801 ggcgatagtt aggctcttca acccagtcgc cctctgttgc ccaccagtga ttaaattcgt
     4861 cgtactttgc tgaaactgcc ataccctatc gcctgtcatt tttattaacg acaatgacta
     4921 taggtggtta cctgaggaaa atcttaatga aacgtgtcgt attaatgaat ttcttttgcc
     4981 actactagcg tgacaccgct tttacccttc atttgcagcg catagattcc accagccact
     5041 accactaata gtgcgcccag cagataaaat gtgaatttaa tcaggcggat acagcgatga
     5101 attttattcg acagggatta ggcatcgcct tacaaccaga gttaacgctg aaaagcattg
     5161 caggtgaatt gtgttccgtt cctctcgaat cgaccttcta tcgacagatt tcgttgttgg
     5221 ctaaagaaaa gccggtagaa ggcagtccac tgtttttact acaaacgtgt acggaacaac
     5281 tcgtggtgag tggaaaaatt tgatgtgaga catgagattg atgacaaatg taagattgcc
     5341 tgattcgcca cacttagttg gcctgtagtc tttaccaggc gtaataattc gttttcacct
     5401 gattttggtc cactttgtga attagcgata tatatttatc cagtttaggg aagtgacaat
     5461 gactccaggt agaaaccggt atgaagcgcc cgactcactg tcaaaaccga ttgttgagag
     5521 taatg
//
LOCUS       DAJOWB010000210         5471 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJOWB010000210 DAJOWB010000000
VERSION     DAJOWB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.210"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1039
                     /locus_tag="OHO85_004621"
     CDS             <1..1039
                     /locus_tag="OHO85_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HCQ2673870.1"
                     /translation="VICLSKQVWENLLIKLKLENNENVFSETKKLSNKNNADQFFECA
                     KRNEQNLFDNIRKSDFHVGLLKPSSTRSVILETPPNVCMESRNSYENKIDEISSLSES
                     KEHPIDIQEKKDAFVNEFKGILFDKNGRSSEFLLNFYECCYEFLPRAQPQDKIESYNS
                     ALQAFSIFCSSTLIHNNIGFDFKLFPEVKLCGENLETVFKYKNGDDVREIAKINITLQ
                     KEEDGLYNLGGLDFKGCFFSGQNFSNYDIQYVNWGTSLFDLDTPCIFNAPAYNKSNEK
                     SLKPVSENGLSGVLTDRNNKIKLITGVAPFDDILFMDDDFDDSSSEDDPVENSPVVTS
                     PVVSSSKSSFQ"
     gene            1425..1613
                     /locus_tag="OHO85_004622"
     CDS             1425..1613
                     /locus_tag="OHO85_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCQ2673871.1"
                     /translation="MDARVVCKPNRLGRSMWHLVVLLEELCKRGINFRALAQSIFAQQ
                     WGDECCKSKRICDLKVIV"
     gene            1768..1887
                     /locus_tag="OHO85_004623"
     CDS             1768..1887
                     /locus_tag="OHO85_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCQ2673872.1"
                     /translation="MLLSYVEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            1952..5207
                     /locus_tag="OHO85_004624"
                     /pseudo
     CDS             1952..5207
                     /locus_tag="OHO85_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
BASE COUNT         1626 a         1044 c         1367 g         1434 t
ORIGIN      
        1 cgtaatatgt ttaagcaagc aggtctggga aaatcttctc attaaactaa agctggaaaa
       61 caatgaaaat gtgttttctg aaactaaaaa attatcgaat aaaaataatg ccgatcagtt
      121 ttttgaatgc gctaaaagaa atgaacagaa ccttttcgat aatataagaa aaagtgattt
      181 tcatgttggt ttacttaagc caagtagtac gcgtagtgtt attttagaaa cgccgccaaa
      241 tgtctgtatg gaatcacgta attcatatga aaacaaaata gatgagattt catctttgtc
      301 agagtcaaag gaacacccca tagatattca agaaaaaaaa gatgcgtttg tgaatgagtt
      361 caaggggata ttatttgata aaaatggaag gtcttcagag tttctactta atttttatga
      421 atgttgctat gagtttttac caagagcgca gcctcaggat aaaatcgaaa gctataattc
      481 agcactgcaa gctttctcca tcttttgttc atctacgttg atacataata atataggctt
      541 tgatttcaaa ttatttccag aagtcaaact gtgtggggaa aatcttgaaa cggtattcaa
      601 atataaaaat ggcgatgatg tccgggagat agccaaaatt aacattactc tccaaaaaga
      661 agaggatggt ttatataatt taggtggatt ggattttaag ggatgcttct tttctggaca
      721 gaacttcagt aactatgata ttcaatatgt gaactgggga acgtcattgt ttgatcttga
      781 tactccgtgt atttttaatg cgcctgctta caacaagagt aatgaaaaat cattaaaacc
      841 tgtgagcgaa aacggtttaa gtggagtctt gactgatcgt aataataaaa taaaactcat
      901 cacgggcgtg gcaccattcg atgatatttt atttatggat gatgactttg atgatagttc
      961 ctctgaggat gatcccgttg agaatagtcc tgttgtgact agtcccgttg tatcaagttc
     1021 taaaagcagt tttcaatgat taataaatgg ctatttttac aaagagataa taacgtagat
     1081 tgaaacaata tgtattattg attgattaaa cgggattatt ttttacacta caaccatttt
     1141 gctgccacta ccaaagaaaa aggggctacg ctttcacgta accccttgat ttatttgttg
     1201 agctggcggt gtctgaattg gtgctgtaat tggttgaaat atttattcta atgtctggtt
     1261 caactttcct tagctgcctg aaagtttttt tgatcttacc catcaataat agggttacgg
     1321 tacaccacgc ctgactgatg aactgcgtgc tcagggttac ctcttcaacg taaaaccgtg
     1381 gcaggaggtc cggactgaaa aaactgctca ggacattatc ggcggtggac gcacgggtag
     1441 tctgtaaacc gaaccgactg gggcgcagta tgtggcatct tgtcgtgctg ttggaggagc
     1501 tgtgcaaacg tggtattaac tttcgtgctc tggcccaatc tatatttgcc caacaatggg
     1561 gggacgaatg ctgtaaaagt aaaagaatct gcgatctcaa agttattgtg tgatttttat
     1621 gtgagcagaa gatattcatc agaaacgatt atgtaaatca ttttattttg ccgacgacct
     1681 gattgtcgaa agaaaagaat acccccgtat tccctgaata gattgacttt tttatccaac
     1741 catacttcag cgcactgcgt ttaaaaaatg cttctttctt atgtggaata tcatcatttc
     1801 atcatgatgt ctttgatgag cggtgaacac aatacacttg cgctgtctct tcaggatgaa
     1861 tcccctggtc tggtgcctgt gggctgatgt tgcagcagag ctaaggtcgc ttaaacgcta
     1921 ctcagtattc acttttcaga ggatgaaatt tatgaacagg accagtcccc attattgtcg
     1981 ccgctcagta ctttccttat tgatatctgc cttgatatat gccccgcccg gtatggcggc
     2041 cttcactcct gatgttattg gtgtggtaaa cgatgagact gtagatggca accaaaaagt
     2101 ggatgaacga ggtacaacaa ataacactca tattatcaac catggccagc agaatgttca
     2161 tggcggggta tctaatggaa gtcttattga atctggtgga tatcaagata taggaagtca
     2221 taacaatttt gtggggcagg ctaataatac aaccattaac ggtggcagac agtcaattca
     2281 tgacgggggt atttccacag gtacgacaat cgagagtggc aatcaggacg tttataaagg
     2341 gggtatcagc aatggaacga caattaaggg cggtgcttca cgcgtagagg gagggagtgc
     2401 gaatggaata ctcattgatg gtggtagcca gatagtaaaa gttcaagggc atgctgatgg
     2461 tacaacgata aataagtctg gctctcagga cgtagtacaa ggaagtctgg caacgaacac
     2521 aaccataaat ggtggtcgac agtatgttga acagagcaca gtagaaacaa caaccattaa
     2581 aaatggcggt gagcaaagag tatatgagag ccgtgcgctg gacacgacga ttgaaggcgg
     2641 aactcagtct ctgaatagta agtcaacggc aaaaattacg cagatctatt ctggtggcac
     2701 gcaaattgtt gataacacca gcacctcgga tgttattgaa gtttattccg gtggcgtgct
     2761 tgatgttagt ggtggtacgg caacaaatgt tacccagcac gatggtgcaa ttttaaaaac
     2821 aaacactaac ggtacgacgg tgagcggtac gaatagtgaa ggtgcattct ccatccacaa
     2881 taaagtggaa gacaatgtgt tgctggaaaa tggaggccat ttagaagtat ctcattccgc
     2941 aaacaagacg attattaaag ataaaggaac aatgtcagtt ttaacgaatg ctaaagctga
     3001 tgcgacccga atagataatg gcggggttat ggatgttgca ggaaacgcga caaataccat
     3061 aattaatggt ggcacacaga atcttaataa ttatggcata gccacaggca ccaatatcaa
     3121 cagcggaacg caaaatatca aaagcggtgg gaaagctgac acaacaatta tatcctccgg
     3181 gagccagcaa gttgttgaga aagatggtac ggcaattggc agcaatatta gcgccggagg
     3241 ctcgctgatt gtctataccg gcggtattgc acatggggtt aaccaggaga cgggcagtgc
     3301 tttagttgcc aacacgggcg cagggactga tattgaagga tacaacaagc tctctcactt
     3361 cactattacc ggaggggagg ctaattatgt tgtgctggaa aataccggcg aactgacggt
     3421 agtggctaaa acctcggcga aaaatactac cgttgatgct ggcggtaagc tgattgtcca
     3481 gaaggaggct aaaacagata ccaccagact taataatggt ggcgttctgg aggttcagga
     3541 cggtggtgag gctaagcatg ttgagcaaca atccggcggc gcattaattg cttccacaac
     3601 ttccggaacc cttatcgaag gaaccaacag ttatggtgat gctttctaca tcaggaattc
     3661 agaagctaaa aatgtagtgc tggaaaacgc tggctcatta acagtcgtca ctggttcccg
     3721 ggcagttgat acgattatta atgccaacgg caaaatggat gtttatggaa aagatgttgg
     3781 cactgtactt aatagtgctg gcacccaaac aatatatgcc agtgccactt ctgataaagc
     3841 aaatatcaaa ggtggcaagc aaacggtata tggtttagcc actgaggcaa atattgaaag
     3901 tggtgaacaa attgttgatg gtgggtcaac tgacaaaacg cacatcaatg gcggcacaca
     3961 aaccgttcag aattatggta aggcgatcaa taccgatatc gtctctggcc tacaacaaat
     4021 tatggcaaac gggatagcgg aaggttccat tattaatggg tgttcacagg tagttaatga
     4081 gggcggtctg gctgaaaact cggtgcttaa tgacggcggc acactcgatg tgcgggagaa
     4141 aggcagcgca acggggatac agcagagtag ccagggcgct ttggttgcaa ccaccagggc
     4201 gacgcgggtc acaggaacac gcgcggatgg cgtcgcgttc agcatcgagc agggtgcggc
     4261 gaacaatatc ctgctggcaa atggcggcgt gttaaccgtg gagtcagaca cctcttctga
     4321 caaaacacag gtcaatacgg gcggacggga gatcgtcaaa acaaaagcca ctgcgacagg
     4381 cacgacgctc accggcggcg aacaaatcgt cgagggggtg gcgaatgaga caacgattaa
     4441 cgacggcgga atacaaacag tttcagctaa cggagaggca ataaaaacaa agatcaatga
     4501 aggcggtacg ctgacagtta acgataatgg caaagcgaca gatatcgtcc agaacagcgg
     4561 tgccgctctc cagacgagca cggctaacgg tattgaaatc agcggtactc accagtacgg
     4621 cactttttcc atttccggca atttagcgac caatatgttg ctggaaaatg gcggtaattt
     4681 attggtatta gcaggtaccg aagctcgcga ctccacggtt ggcagcggtg gagccgccaa
     4741 cggcagttat cgcagctacg ggctgggcgg tcacattgaa accgggatgc gatttaccga
     4801 tggtaactgg aacctgacgc cgtatgcatc gttaacgggg gttcaccgct gataaccctg
     4861 aatatcattt atccaatggc atggaatcga aatcagtcga tacccgcagt ttatatcgtg
     4921 aactgggtgc aacgctgagt tacaacatgc gtctggggaa cggtatggaa gttgagccgt
     4981 ggctgaaggc ggctgtgcgc aaagaatttg tcgatgataa ccgggtgaaa gtgaatagtg
     5041 acggtaattt cgtcaatgat ttgtcgggca gacgtggaat ataccaggca ggtattaaag
     5101 cctcattcag cagtacgtta agcgggcatc ttggggtggg gtatagccat ggtgccggtg
     5161 tggaatcccc gtggaacggg gtagctggtg tgaactggtc gttctgacca tcaacgaaaa
     5221 agcccacatc tgtgggcttt catgtcacca ggagccgcgg ctcctttgcg tatcctttta
     5281 tgtctcttca ccgtctggtc ggtgtcctgc tgagactgct aacttcctgt ttttattggt
     5341 gttgtcctta taccgtccaa tcatgattgg ctggcggagt ctgaatttgt atgttatatc
     5401 tatgttttaa ttggttattt atttaagttg tttttgtttg tatacctaag cgtataccaa
     5461 tagcggggtg c
//
LOCUS       DAJOWB010000211         5408 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJOWB010000211 DAJOWB010000000
VERSION     DAJOWB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.211"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(74..1357)
                     /gene="aroA"
                     /locus_tag="OHO85_004625"
     CDS             complement(74..1357)
                     /gene="aroA"
                     /locus_tag="OHO85_004625"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCQ2673873.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRMRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1428..2516)
                     /gene="serC"
                     /locus_tag="OHO85_004626"
     CDS             complement(1428..2516)
                     /gene="serC"
                     /locus_tag="OHO85_004626"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCQ2673874.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2715..3407)
                     /locus_tag="OHO85_004627"
     CDS             complement(2715..3407)
                     /locus_tag="OHO85_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCQ2673875.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3537..5297
                     /locus_tag="OHO85_004628"
     CDS             3537..5297
                     /locus_tag="OHO85_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HCQ2673876.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1394 a         1393 c         1271 g         1350 t
ORIGIN      
        1 ccgaaaaaaa acgccgacaa tgtgccgacg ttttgataaa gaaagatttg gctatttatt
       61 gcccgttgtt cattcaggct gcctggctaa tccgcgccaa ctgctcgaaa taatccggaa
      121 atgttttggc cgtacatttg ggatcaagaa tcgtcactgg tgtatctgac aacgccacca
      181 gcgagaaaca catcgccatc cggtgatcat tgtatgtcgc gatctcggca aagttcagtt
      241 tttccggtgg agtgatacga atgtaatcgt gcccctcttc tacttccgca ccgactttac
      301 gcagttctgt tgccatcgca aacaggcgat cggtttcttt aacacgccag ttatagatat
      361 tgcgcagcgt ggtggtgcct tttgcaaata acgccgccgt ggcaatggtc atcgccgcat
      421 cgggaatatg gttcatatcc atatcaatag cgttcagttc accacgcgtg caggaaatat
      481 aatcatcgcc ccagcaaatg gtcgcgccca ttttttccag cacatcagca aagcgaatat
      541 caccctgcat actgttacgt ccaataccgg tcacttttac agtgccgcct ttgattgctg
      601 ctgctgccag aaagtaagaa gccgaagatg catcgccttc gaccaaataa gtacccggag
      661 actgataaga ctgcccgcct tttacgacaa attgttgata gtgctgattt tcaatttcaa
      721 caccaaacgt cttcatcaga ttgagtgtga tgtcgatata aggtttagaa accagatcgc
      781 ctttaatacg aatcaccgta tcttccggcg caagaggcgc agtcattaac agtgcggtga
      841 ggaattggct ggaaacggag ccatcaacgt caacgttgcc gccagtaaag ccgccctgta
      901 aacgcaacgg cggataattt tcttgttcca ggtaagtgat cttcgccccg cccaggcgca
      961 gcgcatccac cagatgacca atcgggcgtt ctttcatacg cggctcaccg gtcagcacaa
     1021 tatcattgct acccagacaa agagctgccg ccagcggacg cattgccgtt ccggcgttac
     1081 cgaggaacaa ctccagggca ccttctgcgt gtaatggacc gccgttaccg ataatttcgc
     1141 aacgcatacg atcggctgaa agcgtatagc ttacccctaa cgctgttaat gcattcagca
     1201 tatggcgcac gtcatcgcta tccagcagat tggttaatac tgttttgccg tgtgctaatg
     1261 ccgccagcaa taaagcgcgg ttagaaacgc tcttggaacc gggcagatta atagtgccat
     1321 cgacacgagc gatgggttgt aacgtcaggg attccatgaa ctcaactctc tacaacagaa
     1381 ataaaaaccc cacaggctgg ctgtggggat taagcaaaat ttcggcatta accgtgacgg
     1441 cgttcgaact caaccatgaa gtctgtcagc gctttaacgc cttccagcgg catggcgtta
     1501 taaatagaag cgcgcattcc gccgaccaca cggtgacctt tcagtgcatg aaggccagca
     1561 gcaaaagact cttcaaggaa caatttgtca agcgcactgt ccgccaactg gaacggcacg
     1621 ttcatccgcg aacggttagc tttcgccacg tcattgcggt agaaatcgct gttatcaatc
     1681 accccatata gcagttctgc tttttgctga ttgattttat ccatttcagc tacaccgccg
     1741 ttcgctttca gccatttaaa gaccagacca gatagatacc aggcaaatgt cggcggcgtg
     1801 ttaaacatgg agtcgttatc gttgaggatg gaataatcca gaatcgacgg acacgcgata
     1861 ttcgctttgc ccagcaaatc ttcacgaacg atgacgattg tcaggccagc cgggccgata
     1921 tttttctgcg cgccagcgta aatcacgcca taacggctga cgtcaatcgg acgggaaaga
     1981 atggttgaag agaagtcggc ggcgaccacc acatctttgc cgaagtctgg cgtttcgtcg
     2041 atggcgatac cgtcgatggt ttcattcggg cagtaatgca tataagcagc attatcagag
     2101 agttgccatt cacgcattgg cttaaccgcg cgcagaccat caacagtcac tttggcgtca
     2161 aagacattag gcgtgcagta ttttttcgct tctttaatgg cacttgccgc ccagtaaccg
     2221 gcatcaacat aatctgcggt ggttttatca ccgagaatat tcagcggtac cgcagcaaac
     2281 tgaccgcgac caccgccatg gcagaataat accttgtagt tggaggggac attaagaaga
     2341 tcgcgaaaat ccttctcggc ttcctctgca acctgaatga actctttgcc acggtgactc
     2401 acttccatca ccgacgtacc aagaccgttc cagtcgcgca gttcctgttg agcctgttta
     2461 agcacctctg ccggtagcat tgccggacca gaactaaaat tgaagatttg agccatttcc
     2521 cctcaccacg ttgcgttgct attaacccgc ggtcaatcga ttgcgaccac aggtaataca
     2581 ttattcctgt ggctatcggt tttatcattc agtgacacac atcgcaatga gtaaaaccgt
     2641 tgagggcaaa aatgtggctt gcatcacaca aaagaatctc tcggattatt agcaaaaagc
     2701 cgatgttttt actgtcaggt cacccgtttt gcccgacttg ccttcgtcca ttctggattt
     2761 gcacagcgcg gacataattg tttttccccc gctttcatat gaatgatttc actacaacga
     2821 atacaggcat agtccgccga ctccggcacc actttgtaac gctgcgtaca atcactgggt
     2881 aaaataagta aacccggttt caccttgtca tcttcaaaga aatagacact gatttgcccg
     2941 ttggtttcga gaatcgccag acgtacctgc cccagctgct ccacgccacg caatcgtagc
     3001 tccataaaga actcaaattc cgtcatgttg gagttattga gtttcgacca ggccagctcg
     3061 ccatcttcaa taataacaac cggcttacct tccagtaaat cttccagttt ttcactgtgc
     3121 gccatcaacc acattaccag gcggtataac aacgccagag tgataaagac gataagtacc
     3181 ggcaccatcg gcacatcatc ataaaacgcc acatctcccg ccgctgaacc cagcgtgaga
     3241 atgattaaca cttcaaacag cgacatctgt cgcacaccgc gtcttccggt cattttgagg
     3301 aacaaaaaaa ccagtacaaa ggtataaaga ctacgtagtg caacttcgcc aaggaaatca
     3361 aaaggcactt tatcaaatgc catacggtgg agatcgaacg ccttcatttt tatgctctta
     3421 tcgtggttta tccgctaaaa gcatagtcaa cagttcatcg gcatggcggc ggtgatagcg
     3481 cctttgagat aaaacccgta ttattgcgcg ctttccgtac gactaaagtg attttcatga
     3541 cgcaaacatt tatccccggc aaagatgccg ctctggaaga ttccatcgct cgcttccagc
     3601 aaaaactttc agacctcggc tttcagattg aagaggcctc ctggctgaat cctgtgccta
     3661 acgtctggtc tgtacatatt cgcgacaaag agtgcgcact gtgttttacc aacggtaaag
     3721 gcgcaaccaa aaaagcggcg ctggcttctg cacttggtga atatttcgag cgtctctcaa
     3781 ccaactactt ttttgctgat ttctggctgg gcgaaaccat cgccaacggt ccgttcgtgc
     3841 attatcccaa cgaaaaatgg ttcccactga ccgaaaatga cgatgtgcca gaaggactac
     3901 tcgatgaccg tctgcgcgcg ttttatgatc cggagaatga actgaccggc agcatgctga
     3961 ttgacctaca atccggtaac gaagatcgtg gtatttgcgg cctgccgttt acgcgtcagt
     4021 ccgacaatca gaccgtttat attccgatga atatcattgg taacctgtac gtctccaacg
     4081 gtatgtccgc aggtaatacc cgcaacgaag cacgcgttca gggattgtct gaagttttcg
     4141 aacgctacgt gaaaaaccgc attattgctg aaagcatcag cctgccagag atcccggcag
     4201 acgtgctggc gcgttaccca gcagtagttg aagccatcga aacgctggaa gcggagggtt
     4261 tcccaatctt cgcttatgac ggttcgcttg gcggccagta tccggtgatt tgcgtggtac
     4321 tgttcaatcc tgctaacggt acctgctttg cctctttcgg tgcgcatcct gattttggcg
     4381 tagcactgga acgtaccgtg accgagctgc tgcaaggtcg tggcctgaaa gatttggatg
     4441 tgtttactcc gccaaccttc gatgatgaag aagtcgctga acataccaac ctcgaaacgc
     4501 actttatcga ttccagcggt ttaatctcct gggacctgtt caagcaggat gccgattatc
     4561 cgtttgtgga ctggaatttc tccggcacca cggaagaaga gttcgccacg ctgatggcta
     4621 tcttcaaaaa ggaagataaa gaagtttata ttgccgatta cgagcatctg ggtgtctacg
     4681 cttgccgcat tatcgtgcct ggcatgtccg atatttatcc ggctgaagat ctgtggctcg
     4741 cgaataacag tatgggcagc catttacgtg aaacgattct ttcgctacca ggcagcgagt
     4801 gggaaaaaga agattacctg aacctcatcg agcaactgga tgaagaaggt tttgatgact
     4861 ttacccgcgt gcgtgagctg ttgggtctgg cgaccgggtc ggataacggt tggtacaccc
     4921 tgcgtatcgg tgaattaaaa gccatgctgg cgctggctgg tggcgatctg gaacaggctc
     4981 tggtctggac cgaatggacg atggagttta actcatcagt atttagtccg gaacgcgcca
     5041 actattatcg ctgcctgcaa acgttgttat tactggcaca ggaagaagat cgccagccgc
     5101 tgcaatatct gaatgcgttt gttcgcatgt acggcgcaga tgccgtagaa gccgccagtg
     5161 cggcaatgag cggcgaagcg gcgttttacg gcctgcaacc ggtagatagc gatctgcacg
     5221 cgtttgctgc acatcagtcg ttgttgaagg cctacgaaaa gctgcagcgc gccaaagcag
     5281 cattctgggc aaaataaaat caaatagcct acgcaatgta ggcttaatga ttagtctgag
     5341 ttatattacg gggcgttttt ttaatgcccc gctttacata tatttgcatt aataaaataa
     5401 ttgtaatt
//
LOCUS       DAJOWB010000212         5032 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJOWB010000212 DAJOWB010000000
VERSION     DAJOWB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.213"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..425
                     /locus_tag="OHO85_004629"
                     /pseudo
     CDS             1..425
                     /locus_tag="OHO85_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1098..2606
                     /gene="ltrA"
                     /locus_tag="OHO85_004630"
     CDS             1098..2606
                     /gene="ltrA"
                     /locus_tag="OHO85_004630"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCQ2673877.1"
                     /translation="MQRKLATWAVTDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
     gene            2687..3175
                     /locus_tag="OHO85_004631"
                     /pseudo
     CDS             2687..3175
                     /locus_tag="OHO85_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3626..4498
                     /locus_tag="OHO85_004632"
     CDS             3626..4498
                     /locus_tag="OHO85_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCQ2673878.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            4826..>5032
                     /locus_tag="OHO85_004633"
     CDS             4826..>5032
                     /locus_tag="OHO85_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCQ2673879.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLA"
BASE COUNT         1261 a         1297 c         1371 g         1103 t
ORIGIN      
        1 gcgctcttaa tgtccgactc gctgaacggt tcacgatagc cgccagacta agtgacagcc
       61 acacggttgt cagcctgtgt tctgctctgg aaatacaccg cagcagttac cggtactggc
      121 gaaaacgacg cgatacggtt aatccggcac gagtcaggtt gtgcagcgaa atacgccggg
      181 cgtggaacca aagtcggggc tctgcgggcg cgcgcactct ggctgaaatg ctgactcaaa
      241 acggcgtccc gatgagccgt taccgtgccg gacgtctgat gaaatatctg aacctgagca
      301 gttgtcagcc cggaaaacat cagtataaaa atgcccgtca ggagcatacc agcctgccga
      361 atctgcttga gcgtcagttc gcagtaccgg agccagaccg ggtatggtgt ggagatatta
      421 cgtatcgtgt gcccggagtt cagggcgggc atggatgctt aaatgaaccg cgagtctgtc
      481 tggaatattg aaccggtaac tcacgatgag aaacccaaca atcccaccgg gtgtgacggt
      541 ggagaacctg agcggcagtg acctgcggca tgcccgcagg gtgatgtaac ccgctgacaa
      601 cggggattga ggcgagatca ctaagccgag atgatcctca aggttaagta ctgaaaggct
      661 gaagaacatg aacccgttaa tccgcctctg tgggttgaaa acgtcaccac ggcctacgtg
      721 atctgacagg ccgtgcagga ggaactggca gtgatacgta agcactgccg gtcgaaggtg
      781 ttttgacatg tatgcgaaac accggggcag cagcgtctat cacgcttgcg ttgctgactt
      841 ctgccaactt gcggcaagca aggataaaga gtgcgacggg cagcctcctc agtatgcctg
      901 agtccaggca ggtaaaccgg ggaaggtcag cgacggatgt taagggggca tggctccgat
      961 gacgcgctgg ctggcggagc ttccgtagta gtccgcgatg gggaaagccc attacatggc
     1021 gaagggaagc agtttgaatg tgtttgcgac gtgaattaac tgacctaacg aggtgaagac
     1081 ctttgataat cagcgaaatg caacgcaagc ttgccacatg ggcagtcacc gatccgtccc
     1141 tacggattca acggctgctg cgtctgataa cacaaccaga atggctggct gaagcggcgc
     1201 ggatcacgct ttcatcaaag ggggcccata cccccggcgt tgatggcgtg aacaaaacaa
     1261 tgctacaggc cagactggct gttgagctgc aaatcctcag ggatgaatta ctctcaggcc
     1321 actaccagcc cttgcccgcc agacgggttt acatccctaa aagcaacggc aaactgcgac
     1381 cactgggtat ccccgcgttg cgggatcgta ttgttcagcg ggccatgctg atggcgatgg
     1441 agccgatatg ggagagtgat tttcatacgc tctcatatgg cttccggcct gagcgcagtg
     1501 tccaccacgc tatccgcacg gtgaaattac agctcacaga ctgtggtgaa acccggggac
     1561 gctgggtgat tgaaggcgac ctgtccagtt acttcgacac cgtacatcat cgactgctga
     1621 tgaaggctgt acgccgcagg atcagtgacg cacgtttcat gactctgctg tggaaaacca
     1681 tcaaggcggg acatatcgat gtcggtctct ttcgggcggc cagtgaaggt gtgccacagg
     1741 gcggtgttat atcgccgcta ttatcgaaca tcatgctgaa tgagttcgat caatacctgc
     1801 atgagcgcta cctgagcggg aaagccagaa aagatcggtg gtactggaat aacagtatcc
     1861 aacggggccg aagtacggcg gtcagagaaa actggcagtg gaaacccgcg gtggcgtact
     1921 gccgctatgc cgatgatttt gtcctcatcg tcaaaggcac caaagcacag gcggaagcca
     1981 tcagggagga gtgtcggggt gtgctcgaag gcagtctgaa actcaggctg aacatggata
     2041 agactaaaat cacccatgtt aatgacggct ttatctttct ggggcacagg atcattcgca
     2101 aacgcagtcg ttatggcgag atgcgagtgg tctcaacgat cccgcaggag aaagccagaa
     2161 acttcgccgc atcgctgaca gcactgttat caggcaacta cagtgaaagc aaagtcgata
     2221 tggctgaaca actcaaccga aaactgaaag gctgggccat gttctatcag ttcgttgatt
     2281 ttaaggccaa agtcttcagt tatatcgacc gtgtcgtgtt ctggaagctg gctcactggc
     2341 tggcccgcaa ataccgtaca ggtatcgctt ccctgatgag gtggtggtgt aaatcaccga
     2401 aaccgggtca gagcaaaacg tgggttttat ttggtaaaac caatcacggc aagctcagcg
     2461 gcgaaatact gtaccggttg gtggggcaag gcaagaagct gttccgctgg cggctacccg
     2521 aaggtaatcc ctatctgagg acggagacca gaaacacgta tacatcgcgc tttacagaag
     2581 tggcaatggc gttcgccagc atttaaatgg agagccggat gcgctgaaag gtgcacgtcc
     2641 ggttcgggga ggagaggcag ggaaatagtc cgactacgcc ctgcctctta ctctactctg
     2701 ggcaggaaat cgctggtgct atctggcggt tgttatggat ctttttgccc gcagggttat
     2761 cggctggagt ctgtcagcgc atgccgatac cgcactgata agcagtgccc tgcggatggc
     2821 ctatgagacg cgtggccaac cccgggatgt cgtgttccat agcgaccagg gaagccagta
     2881 tacaggcctt aaatatcaac aacttctctg gcgttgcaga ataaatcaaa gcgtcagtcg
     2941 gcggggaaac tgctgggata acagccccat ggaacgcttc ttccgcagtc tgaaaacaga
     3001 atgggtgcca acgaatggtt acgcaggcaa ggacgaggcc cggcagcaaa ttaatgatta
     3061 catattgaac tactacaaca gcgtcagacc tcaccattat aacggtgggc tgacgccgga
     3121 agagtcagag aacagatacc atttttactg taaaaccgtg gccaatatta cttgaccact
     3181 acacacaaaa gcatttaatc attacgcatg accaggaagg gaaacactta accacatggt
     3241 atcaacagat atcattacgt gtgcactctt ataaaaaaca cacagaagaa gtaagataat
     3301 tataaatgta aatttaatat ctcccactga catctccccc acaatacact gaaaaatact
     3361 cacagcagta aacacagaca cacctgataa tcatgcagcc gataaatatt ttccccttta
     3421 cggagaaatc agcctgctgt ttaacaataa cggcgtattt tcatggaagg agatcaccca
     3481 gtaacggata agtcctgatc tccttcatgt taatacgcaa caatcccatc gattaaaaac
     3541 aatttcagac agatattatc tccgtgaatc ccccgccacc tttccggtgc gcggggtttt
     3601 gtcttttttc accgggaata catgtatgaa tccgtctgat gccattgagg caattgaaaa
     3661 accgctctcc tccctgcctt actcgctttc ccgtcacatc ctggaacatc tgcgcaaact
     3721 cacccgtcac gaacccgtga ttggcattat gggtaaaagc ggggccggta aatcctcact
     3781 ctgtaatgca ctgtttcagg gggaggtcac cccggtcagt gatgttcacg ccggcacccg
     3841 ggaagtacgg cgcttccgtc tgagtggcca cggtcacagc atggttatca ctgacctgcc
     3901 cggggtgggc gagagccggg acagggatgc agagtatgag gccctgtacc gtgacattct
     3961 gcctgaactt gacctggtac tgtggctgat taaagccgat gaccgtgccc tgtctgtgga
     4021 tgagtatttc tggcgacaca tcctgcaccg gggacatcag cgggtactgt ttgtggtgac
     4081 gcaggccgac aaaacggagc cctgccatga atgggatatg gccggtattc agccttctcc
     4141 tgcacaggca cagaatattc gcgaaaaaac ggaggcggta ttccgtctgt tccggcccgt
     4201 acatccggtt gtggccgtat cggcccgcac cggctgggaa ctggatacgc tggtcagtgc
     4261 gctcatgaca gcgcttcccg accatgccgc cagtcccctg atgacccgac tgcaggacga
     4321 gctgcgcacg gaatctgtcc gctctcaggc ccgtgaacag tttaccggtg cggtggaccg
     4381 gatatttgac acagcggaga gcgtctgtgt tgcctctgtt gtccgtacgg ccctgcgcgc
     4441 tgttcgtgac acggtggtct ctgttgcccg tgcggtatgg aactggattt tcttctgaac
     4501 ctgtcgtgac tgatgccctc cctgactctg agtctgctca caaaagcact gttttcgtta
     4561 ctgtctctct tgtccgtgca atagctcaat aatagaataa aacgatcgat atctatttta
     4621 tcgatcgttt atatcgatcg ataagctaat aataaccttt gtcagtaaca tgcacagata
     4681 cgtacagaaa ggcattcagg gaacaacaga accacaattc agaaactccc acagccggac
     4741 ctccggcact gtaacccttt acctgccggt atccacgttt gtgggtaccg gcttttttat
     4801 tcaccctcaa tctaaggaaa agctgatgaa acgacatctg aatacctgct acaggctggt
     4861 atggaatcac attacgggcg ctttcgtggt tgcctccgaa ctggcccgcg cacggggtaa
     4921 acgtggcggt gtggcggttg cactgtctct tgccgcagtc acgtcactcc cggtgctggc
     4981 tgctgacatc gttgtgcacc cgggagaaac cgtgaacggc ggaacactgg ca
//
LOCUS       DAJOWB010000213         5006 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJOWB010000213 DAJOWB010000000
VERSION     DAJOWB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.214"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            194..2041
                     /gene="ldtD"
                     /locus_tag="OHO85_004634"
     CDS             194..2041
                     /gene="ldtD"
                     /locus_tag="OHO85_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCQ2673880.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2222..2770
                     /locus_tag="OHO85_004635"
     CDS             2222..2770
                     /locus_tag="OHO85_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCQ2673881.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILKLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2797..3444
                     /gene="gloC"
                     /locus_tag="OHO85_004636"
     CDS             2797..3444
                     /gene="gloC"
                     /locus_tag="OHO85_004636"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCQ2673882.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3666..4856)
                     /gene="aspC"
                     /locus_tag="OHO85_004637"
     CDS             complement(3666..4856)
                     /gene="aspC"
                     /locus_tag="OHO85_004637"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCQ2673883.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSASLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
BASE COUNT         1234 a         1245 c         1315 g         1212 t
ORIGIN      
        1 acattacggc gcgcttttct ttaaacttct ttacattagg ttatgtaaaa acgtatcggc
       61 gtttatatac tgaagataag cctgatgagt aacaggcttg ctcgtcatac tttcgtgagt
      121 attggcgttg tacaggcaag tcgtaaaata acagcctggc tattcagagt atgataaaaa
      181 cagggggcaa gggatgttgc ttaatatgat gtgtggtcgt cggctgtcgg caatcagttt
      241 gtgcctggcc gtaacattcg ctccactgtt caatgcgcag gccgatgagc ctgaagtaat
      301 ccctggcgac agcccggtgg ctgtcagtga acagggcgag gcactgccgc aggcgcaagc
      361 cacggcaata atggcgggga tccagccatt gcctgaaggt gcggcagaaa aagcccgcac
      421 gcaaatcgaa tctcaattac ccgcaggtta caagccggtt tatcttaacc agcttcaact
      481 gttgtatgcc gcacgcgata tgcaacccat gtgggaaaac cgtgatgctg ttaaagcctt
      541 ccagcaacag ctggcagagg tggcgattgc cggtttccag ccgcagttta ataaatgggt
      601 agagttactg accgatcctg gtgttaacgg gatggcacgc gacgtagtgc tctctgatgc
      661 gatgatgggc tatctccatt tcattgcaaa tattccggtc aaaggcactc gctggctata
      721 tagcagtaaa ccttatgcac ttgcaacgcc gccgctctcg gtgattaacc aatggcagct
      781 ggcgctggat aaaggtcaat tgcctacgtt tgttgcagga ctggcaccgc agcatccgca
      841 atatgcggcg atgcatgaat cgttactggc cttactcagt gacaccaaac cgtggcccca
      901 actgaccggc aaagcaacgt tgcgcccagg gcagtggagt aacgacgtac cggcgttgcg
      961 cgaaatattg caacgcacag gcatgttgga cggggggccg aaaattactc tacctggcga
     1021 tgacacgcca actgacgcgg tagtcagccc atccgctgtt actgttgaaa cagcagaaac
     1081 taagccgatg gacaagcaaa cgacgtctcg tagtaaacct gcgcctgccg ttcgtgccgc
     1141 ctacgataat gaactggtgg aagccgttaa acgttttcag gcatggcaag gattgggggc
     1201 agatggtgct attggcccgg caacacgtga ctggttaaac gtaacgcccg cccagcgtgc
     1261 tggcgtgttg gctctcaaca tccagcgatt gcgcttgctg ccaacagagc tttctaccgg
     1321 gatcatggtt aacattccgg cctattcgct ggtctactat cagaacggca atcaggtgct
     1381 ggattcgcga gtcattgtcg gtcgccccga tcgcaaaacg ccgatgatga gcagtgccct
     1441 taacaacgta gtggtaaacc cgccgtggaa cgtacctcca actctggcac gcaaagatat
     1501 tctgccaaaa gtgcgcaacg atccgggata tctcgaaagc catggctata cggtgatgcg
     1561 cggctggaac agcagagaag cgattgatcc atggcaggtt gactggtcta caatcacggc
     1621 ctcgaattta ccgttccgct tccagcaggc tccaggccca cggaactcgc tggggcgcta
     1681 taaattcaat atgccgagtt cagaggccat ttatttgcat gacacgccga accacaatct
     1741 gttcaagcgt gatacacgcg cattgagctc aggctgtgta cgagtgaata aagcttccga
     1801 tctggcgaat atgctgttgc aggatgcagg ctggaatgac aaacgtattt ctgatgcgct
     1861 gaagcagggt gatacacgtt acgtcaatat tcggcagtcg attccggtga atctctacta
     1921 cctgacggcc tttgttggtg cagatggtcg tacccagtat cgtacagata tttacaatta
     1981 tgatctgcct gcgcgatcca gctcgcaaat cgtatcgaaa gcggaacaat taatcaggta
     2041 aatgaagtag ttcggggaaa tcagttgtcg taataattag catgattggg ggcgattctc
     2101 tgcagccccc gtcactgctg gggttgagtc atcttgacgt ctgctttacg ggcggttaag
     2161 gtgcctcttg tgcgccagaa gtgcatataa acgataacat tgacctgtag acttgattat
     2221 catggacaaa ttcgacgcta atcgccgcaa attgctggcg cttggtggcg ttgcactcgg
     2281 tgccgccatc ctgccgaccc ctgcgtttgc aacactctct accccacgcc cgcgcatttt
     2341 gaaactcaat aatcttcata ccggagagtc aatcaaagcg gagtttttcg atggcagagg
     2401 ctatattcag gaagaattgg caaaacttaa ccattttttc cgcgattacc gcgcgaacaa
     2461 aataaagtcc atcgacccag gattattcga ccagttgtat cgcctgcaag ggttgttagg
     2521 cacgcgcaaa ccggtgcaac tcatttccgg ttatcgttcc attgatacca acaatgaact
     2581 acgcgcccgc agccgtggag tagcgaagaa aagctatcac actaaaggcc aggcgatgga
     2641 tttccatatt gaaggtatcg cgttaagcaa tattcgcaaa gccgcgttat ctatgcgcgc
     2701 aggtggtgta ggatattacc cacgtagtaa ctttgtgcat attgataccg ggccagcacg
     2761 gcactggtag taattgctta acgaaacagg ggcagtatga actatcgtat tattccggtc
     2821 accgcattct cccagaactg ttcattgatc tggtgtgaac aaacccgcct ggccgcactg
     2881 gtcgatcctg gcggcgatgc ggaaaaaatc aaacaggaag ttgatgacag cggcctgaca
     2941 ctgatgcaga tcctgctgac gcatggtcat ctggaccacg ttggtgcagc ggcggaactg
     3001 gcgcaacatt acggcgtgcc ggtttttggt ccggaaaaag aagatgagtt ctggctgcaa
     3061 ggcttgcctg cgcaaagtcg tatgtttggt ctggaagagt gccagccgct gacgccagat
     3121 cgttggctga acgaaggcga taccatcagc atagggaatg tgactttaca ggtgttacat
     3181 tgccctgggc atacgccggg tcatgtcgtg ttttttgatg atcgggcaaa gctgctgatt
     3241 tctggcgatg ttattttcaa aggcggagta gggcgcagtg acttcccgcg tggcgatcat
     3301 aatcaactga tttcttcaat caaagataaa ttgctgccac tgggggatga cgtgacattt
     3361 attccgggtc acggaccatt atccacactt ggttatgaac gcctgcataa tcccttcctg
     3421 caagacgaaa tgcccgtctg gtaaggcaca taaaaaagcc cgcttttaat gcgggcctgg
     3481 atttctgaca aagtgcgctt tgtttatgcc ggatgcggca cgagcgcctt atccggccta
     3541 caaaatcgtg caaattcaaa atattgcagg ggacgcgtag gcctgataag cgtagcgcat
     3601 caggcaatgt tgcgtttgtc atcagtctca gcccgctttt cagcgggctt cattgttttt
     3661 aatgcttaca gcactgccac aatcgcttcg cacagcggag ccatgttatc tggtgtcatc
     3721 ccggccacat ttacgcgacc agaagcaacc gcatatacgc caaactcttc gcgcagacgc
     3781 agcacttgtt cttttgtcag gccactgaag gagaacatgc cgttctgttt gatgataaag
     3841 ctgaagtcgc ggtttgcgcc tttttcctgc agcgtattga cgaacaactg acgcatacgc
     3901 tgaatacgct ggcgcatatc agtcagctct tgttcccaaa tcgcacgtaa cgcatcgttg
     3961 ctcaggatgg tggcaacaac agaagcgccg tgtgctggtg ggttagagta gttagcgcga
     4021 atcgccgctt tcatttggct gaatgcgcga tcaacggttt cactgtcggc agcaaccaga
     4081 gtacaagcgc caacacgctc gttgtacagg ccaaagtttt tagagtagga actggcaaca
     4141 atcagctctt tatgcatagc cgcgaaagcg cgcagtcctt cagcatcttc ttccagacca
     4201 cgggcaaaac cctggtaagc gaagtcaaac agcggtaacc agcctttctc aactgagagt
     4261 tgtgccagtg tctgccattg ttccagcgta gggtcgatac cggttgggtt atggcagcag
     4321 ccatggaaca gcactacgtc gccagcctga gcttcgttca ggctgttaat cagtgcatcg
     4381 aagtcaagag tgtgattttc cgcatcataa taagcgtatt cacgaacttc cagacttgca
     4441 gagttaaaga cgctcttatg gttcggccag cttgggttgc tcacccacac tcgcttaacg
     4501 ctggtatttt ttgccaggaa atcggcagct acgcgtagtg cgccagtgcc ccccggagtc
     4561 tgtgccgtgc gagcacgttt gtcattgatc agggcgctac ctttaccaaa cagcagttcc
     4621 tgagtgcagc gaccaaattc agggatgccg tcaatgccga ggtaattttt ggtggtttca
     4681 ttttcgagca gatactgttc agcctttttc acgctggtca gtaccggggt tttgcccgtc
     4741 tcatctttat agacaccaat cccgaggtta attttgccgg gacgttcatc ggcacgaaac
     4801 agatcggcca ggcccagaat cgggtcggca ggagcggcgg taatgttctc aaacatgacg
     4861 aggttccatt atggttacag aagggaagtc cgctatcagg gtaacgggag atttacaaaa
     4921 ttccaactat tactgatgaa aacgcaggct gtttttgcaa gacgtgagat tgctctggaa
     4981 ggtataaaaa aaacaggacc aaagtc
//
LOCUS       DAJOWB010000214         4939 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJOWB010000214 DAJOWB010000000
VERSION     DAJOWB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.215"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(331..2148)
                     /locus_tag="OHO85_004638"
     CDS             complement(331..2148)
                     /locus_tag="OHO85_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012967631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCQ2673884.1"
                     /translation="MPLRSVSAAYLAQAAELAGNPGQLAAYESRGHCVVLAGPGSGKT
                     KTLVLKLARILAEDVQAPRGAACITYSQECARELTRRLEALGLREAPNLFIGTVHGFC
                     LRHLLMPYGRLADLPIPFPLSVASQRASERLLRQSGDRLFGPNHPYKAIDLGRHRRSV
                     LNRNSVAWRAEEELAAWAEAYEAALRQEGLIDYDDMVVFGQRLIVEHDWVLPLVQAKF
                     PILAVDEYQDLGVALHRIVKRLAFEGGVRLFAVGDADQSIYGFTGADGALLMELASRR
                     DVETVRLQLNYRSGTGIVNASEMVLGEVRGYRASDPNRQTTIAFQLCPDGLEDQAVHA
                     VAQIIPTALAAKPGRTLGDIAILYKDYRAGDVVADAVTAGGFDYIRVDTAAPYRKVAL
                     TSWVEDCAAWCAGGWRIGRPQLRGLIDRYLAFHRARLDDACARRKGQELTRLLWALRT
                     DQQPACDFVVSLRNGLVDSLLATEPALSDQAEQLEHMTAALAEGGALALLDITSLGGR
                     DGSPEHLNLLTLHSAKGCEYDVVIMVGLDLGNLPWRNEAPEKLRESRRLFYVGLTRAR
                     DEVHMLYSGFVDGRYGPMRLGRSPFLDELEARMRGAGLW"
     gene            complement(2135..3919)
                     /locus_tag="OHO85_004639"
     CDS             complement(2135..3919)
                     /locus_tag="OHO85_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017640421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCQ2673885.1"
                     /translation="MRISRIRLINFANFSDVDVETGESIVIVGENKVGKSNFIRGLQL
                     ILDPALSERDRQLGFEHFWDGLGEDKLGETIEISVDFTDFTDDPRLMAHLNDCVLNPG
                     PPMVARLTYRFQPKTELNRAPESLKDYEYVIFGGADPDMHIGGAFRRMLPIDVQGALR
                     DAEKDLSSWRNSPLRPLIEELSASLDEEDREEIQTLVDEAQRELAGHDEVVATAERIS
                     ERLIAIAGEQHAVPVSLGLAPTRVDALLRSLRLLLDSGIRGIGDASLGTANLIFLALK
                     SLELDRLVDDGERDHTFFVVEEPEAHLHPHVQRLVYRYFLGTDGENGDERAPLTTILT
                     THSPHIASVTPVRSIVLLRHDAEDEKTIAVSTANAPFTQRDEDDLQRYIDVTRGEIFF
                     SRGVILVEGDAERFLVPAFADALDIPLDMLGITVCSIGGTNFTPYVKLLGPRGLNIPH
                     VILTDRDPMAVGEPLVRRRLINILDVLEGDVDHDDMDTDEVIARAGVYGCFVNEITLE
                     PELFTGGLAEAMQSVIERELSLAQVTLDRIQAWVDDTDQLDIARLLKLIERIGKGRFA
                     QALATSVSEDVCPDYIREALEHIRDAVA"
BASE COUNT         1260 a         1398 c         1160 g         1121 t
ORIGIN      
        1 gaagagcccg ctcagggaaa cctgagcggg ctcttgcttt ggtttgattg gtgaatcgca
       61 acagactcaa attttatcca tcaaataacg atgcgttttc gatgccatgc tggcggcttt
      121 gaaattagag cgtttatcgt ttttcatcgc ttttaacggg tagcaaacca tctttcggtc
      181 cagtttcagt cgcaggtgat gatttacctg ttcagtggac taaattatgg aagatgcatt
      241 gtgcctttgg ttcagcattg gaaggtggcg caattatcgg cgttttcttt tgctggctgt
      301 ccacagattc gatcacgcat ttattcccga ctaccataaa ccggcccctc gcattcgcgc
      361 ctccagttca tccagaaatg gggaacgccc tagacgcata gggccgtagc gaccatcaac
      421 aaaaccggaa tacagcatat gcacctcatc ccgtgcgcgg gtcagcccca catagaaaag
      481 acgacggctt tcgcgcagct tctctggcgc ctcattacgc cacggcaaat tccccagatc
      541 caaaccgacc atgatgacca cgtcatattc gcaacctttg gctgagtgaa gcgtcagcaa
      601 attgaggtgt tcaggtgagc cgtcacgacc acctaggctc gttatgtcca gcagcgccaa
      661 cgcaccgcct tcggcaagtg ctgcagtcat gtgttccagt tgctcagcct gatcagaaag
      721 tgccggttca gtagccagca gggaatcaac gagtccgttg cgcaacgaca cgacaaaatc
      781 gcacgcaggt tgctggtcgg tgcgaagtgc ccaaagcagc ctagttaact cctgtccctt
      841 gcgccgggcg catgcatcat ccagtctcgc ccgatgaaat gccagataac ggtctatcaa
      901 tccacgcagt tgagggcgcc caatacgcca accaccagca caccatgccg cgcaatcctc
      961 cacccagctc gtcaaggcaa ccttgcgata aggtgctgcg gtgtccacac gaatgtaatc
     1021 aaaaccgcca gcggtcaccg catcggccac cacatcaccg gcacggtagt ccttgtacag
     1081 aatggcgatg tcacccagcg ttcgacccgg cttagcagct aacgcagttg gaataatctg
     1141 cgcgacagca tggacagcct gatcctccag accatccgga cacagctgga acgcaatggt
     1201 tgtctggcgg ttcgggtcgc ttgcgcgata accacgaacc tcacccaata ccatctcaga
     1261 tgcattgacg atgcccgttc ctgatctata gttcaactga agcctgactg tttcaacgtc
     1321 tctcctgctt gccagttcca tgagtaacgc accatcggca ccagtgaaac cgtagatcga
     1381 ttggtctgca tcacccacag caaataagcg tacgccaccc tcgaaagcca ggcgtttgac
     1441 tatacggtgg agcgcaacgc ccaaatcctg atattcatca accgcaagga tcggaaattt
     1501 cgcctgtacc aagggtagaa cccagtcatg ctcgacgatc agacgctggc cgaataccac
     1561 catatcgtcg tagtcgatta gcccttcctg ccgcaatgcc gcttcgtagg cctcggccca
     1621 ggcagcgagt tcctcctctg cacgccaggc aacactgttg cggttcagca ccgaacggcg
     1681 atgtcgtccg agatcaatag ccttataggg atggtttggg ccaaagagac ggtcaccaga
     1741 ttgtcgaagt aaccgctcgc tagcacgctg agaagctact gaaagcggaa atgggattgg
     1801 caaatcggcc agtcgtccat agggcatcaa cagatggcgc aaacagaacc catggacggt
     1861 tccgataaag agattcggtg cttctctgag ccccagcgct tcgagacgac gggtcagctc
     1921 gcgagcacat tcttggctat atgtgataca tgccgcgcca cgtggagcct gaacatcctc
     1981 ggccaggatt cgggccagct tcagcacgag cgtcttggtc ttaccactac ctggcccggc
     2041 gagtaccacg caatgcccac gggattcata cgcggctaac tggcccggat tcccggccag
     2101 ctcggcagcc tgagccaaat aggctgcact gacactacgc aacggcatcg cggatatgct
     2161 ccagtgcttc gcgaatgtaa tccggacaaa catcctccga caccgaagtt gccagcgcct
     2221 gggcgaaccg ccctttgcca attcgctcaa ttagtttcaa caaccgggct atatcaagct
     2281 ggtccgtgtc atccacccat gcctgtatcc gatctaaggt aacttgcgcc agagatagtt
     2341 cccgctcaat gaccgactgc atggcttcgg ccagtccacc ggtaaacagc tcaggctcca
     2401 aggtgatctc attgacaaag catccataca ccccggcacg tgcaatcact tcatcggtat
     2461 ccatatcatc atggtcaaca tctccctcaa gcacgtctaa aatattgata agtctcctac
     2521 gaaccagcgg ctctccaacc gccataggat cgcggtctgt caaaatcaca tgcggaatat
     2581 taagcccccg tggccccagg agcttcacat acggagtgaa atttgttcca ccgatcgagc
     2641 acaccgtgat cccaagcata tcgaggggaa tatcaagagc atcggcgaat gcagggacaa
     2701 gaaagcgttc agcatcccct tccactagga tcaccccacg tgaaaagaat atttcgccgc
     2761 gggtgacgtc gatatagcgc tgcagatcat cttcgtcccg ctgcgtaaac ggtgcgttcg
     2821 ccgttgaaac agcaatagtc ttctcgtcct ctgcgtcatg acgcagcaga acgatagatc
     2881 tgacaggcgt gacgctggca atatgtgggg aatgggtggt caggatcgtt gtcagcggtg
     2941 ccctttcatc accattctcc ccgtcagtac ccaggaaata acggtagact aggcgctgta
     3001 cgtgcggatg caaatgtgcc tcaggctcct cgacgacgaa aaacgtatgg tctcgctctc
     3061 cgtcatcaac aagacgatca agttcgaggc ttttcagagc cagaaaaatc agattagctg
     3121 tccccaagct ggcatcgcca atgccccgaa tgccactgtc gagcagcaaa cgcaggctac
     3181 gtagcagcgc atcaacgcga gtcggtgcaa gtccgagcga taccggaacg gcatgttgct
     3241 cacctgctat agcaatcaac cgttcgctaa ttcgttcggc tgtcgccacc acctcatcat
     3301 gcccagccag ttcccgctga gcctcatcga ccagggtttg aatctcctca cgatcctcct
     3361 cgtccagaga tgcggaaagt tcttcaataa gcggcctcaa gggtgaattg cgccaacttg
     3421 aaagatcctt ctcagcatca cgcaacgcgc cttgaacatc gatgggcaac atacgacgaa
     3481 acgcaccacc aatatgcatg tcaggatcag caccaccgaa gatcacatac tcataatctt
     3541 ttaacgattc tggagctcga tttaactcgg ttttgggctg aaagcgatag gtaaggcggg
     3601 ccaccatcgg aggccccgga ttaaggacac agtcattaag atgagccatc aatcggggat
     3661 cgtcagtgaa atcagtgaaa tctactgaaa tttcaatggt ctcccccaat ttgtcttcgc
     3721 ccaatccatc ccaaaaatgt tcaaaaccca gttgacggtc acgctcagac aacgctgggt
     3781 caagaatcag ttgcaggccg cgaataaaat tgctcttgcc aaccttgttt tcaccaacaa
     3841 taacaatact ctcacccgtt tcaacatcaa cgtcggagaa gttggcgaaa ttaattaacc
     3901 ggattcggga aatccgcatt actgcctccc tatctttttg attatcttcc aaaataagag
     3961 tagccctcaa accgcaagag actgcctagc acgaccaaat ctgctgaggg gtgcagttgc
     4021 gatcaaaacg gttttggtca ttgcacattt tttgctaatc aaatctattt gtccgacgaa
     4081 ggtaacagtt catcgtaaca gagagctaaa ccatgcctat gatccatctc acaaagtggc
     4141 tttttgtcgt cactgccttg ctcagtggct gcgcgacaac ctcctcgcct ctcttgcctt
     4201 cacccattaa agagatttct ccggttcctg aaaatgctga gcccggtttt attccgggcg
     4261 cgaccctctt taaagctagc acctgatgta gcgcagcacg acctgatatt gcaggtaatc
     4321 ggcgtggcat tgccggaaaa ctcccgcgct accgttggtg acggtctgca aggcgcgcta
     4381 aggcgaagcg gctatcagac tctattcact gtcactgccc gtggcactct tgtatccggc
     4441 ctaatgagcc tgagctacgc attgctcaca ttggacgggc catcctggga tttggagaac
     4501 aatgagctgg cacgacaggt cataccctgg cgatacggca caatatcaac tggaagcaga
     4561 tcaatttttc tcagggcatt aggtataatg attctaaacc ctcatgtcta tagcgtgcat
     4621 actgcctcct atactgctga atattttagg gactcagctt ggcggggagt gactttcttc
     4681 taatgaaaat attacttact taatgtcgta aaaaaattat tctcttgggg atatacgcct
     4741 tctttgtcca ctaaatatta ttcgaccata caaaaacagc cttcaatatc aacagttaga
     4801 taccaactat gatgatattt ttagtggaaa actgcaagat gcggtttatc aatgccacag
     4861 ggtaaaggga cagtaactta tggtactcga aagattacca ctagtgggaa tcaggatttc
     4921 aaataacaag atatgtgca
//
LOCUS       DAJOWB010000215         4927 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJOWB010000215 DAJOWB010000000
VERSION     DAJOWB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.216"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            69..821
                     /locus_tag="OHO85_004640"
     CDS             69..821
                     /locus_tag="OHO85_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCQ2673886.1"
                     /translation="MIRIYKASKGQLKHREIFDEIKKKIDNGEWKEGKAIPTERELAE
                     HFLSSRTTIRKAIESLKQRGYLHSVHGQGTFTLPARSRENHLLHSFTDDIKARGGVPA
                     QNILEIGYIPLSDIIRKNLELDIHVHTVFCIKRIRYMGSTPLGIQTSWLALNDGQEIT
                     QEELLATGSLYILLEEKLAIKPLEATEIISARLPSPLERQLLELTADDVVLSCTRVTL
                     SVERKPMEYVEMVYPASRYSYKARITKDSFSV"
     gene            835..1911
                     /locus_tag="OHO85_004641"
     CDS             835..1911
                     /locus_tag="OHO85_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCQ2673887.1"
                     /translation="MNKIEQAVIYNKILGSLACAGMGDALGAATELYSIDEIKAQWGG
                     FLNAFVSPPADTFAGSLNGIAGLITDDSSQMYVFSEGLVEAGFDNFTNNDWLACLLRW
                     ADMQPYANYKGPTTEQIVKALKEGRPTNTIGRIGTSSRQAPNVGTTNGAGMRVAPAGL
                     IWPGKKEKACHLALITCLPSHDTNIAIASACAIAAATSQAMLPEASLTSLLDAAIWGA
                     NYGERLAKQYARCVAGPSIAMRIQLAADIARRANDLESCLREMEGLVGNSVAAHESIP
                     AAIGLLLYCKGEPWETIHACANIGNDTDSIATMAGAIAGAWRGFDALPEDKYAFFRAV
                     NNKDFDIEAIASGLTLLALQAQEK"
     gene            1911..2909
                     /locus_tag="OHO85_004642"
     CDS             1911..2909
                     /locus_tag="OHO85_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCQ2673888.1"
                     /translation="MNMPARTPVMPLAQTREEINATAASLRHLAAIWGECFSTFQLDK
                     RAVERVCIVGSGDSWTVALCAAAWLGKYTHLFCYALQTWDFLQTDLTRYQKETLIIIL
                     SASGRPSMTVDALCHAVCSNAQVLGVTNFPGTPFCAITENMLYTWANKQGIPTQSSSV
                     TLYALLRLAQKLCPGLTPLQIEEDLEGKFSQINQHWQQKKRHFYQQKEITFLGSGLSW
                     GLAISGSNLLSCGPQIRATALPLEEFYHSLRLHQAGVGQHYILLPATSCESPFYLATQ
                     KAIVEQGATAELISFIPDASESNNLFLVMQWLYEMCWHLSCDYVDAGGQRVSHREK"
     gene            2914..3828
                     /locus_tag="OHO85_004643"
     CDS             2914..3828
                     /locus_tag="OHO85_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCQ2673889.1"
                     /translation="MKKFDVAALGSGNIDIFLSIPSLPTRGGKVVGTRLGEQAGGTVA
                     NSACAMGQLGLNVVSVSCVGNDHSASIILDGFKKYHVNCDFVQVIPELIANTAIIFID
                     ELGEKTLVYSPGSDHEWDKEKALQAIAQSRYFYTMPADIEKFRMLAEYSRSQMTKVVV
                     DIEPHIIATPEHLARILHLADIAIFNYDGFIRGYATEPDFTLLHDIQDEYQLDAVVVT
                     LDVRGVIAVKGNEQVKIGSYTIPVIDTTGAGDTFNGAFVYSLIKNMPLIEALKFASAT
                     AAINITALGARGHLPTPSEVNLFLSQHD"
     gene            3856..>4927
                     /locus_tag="OHO85_004644"
     CDS             3856..>4927
                     /locus_tag="OHO85_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCQ2673890.1"
                     /translation="MKIRYAAIFLALPLLCTTAQANSFLHYAGEFNFRTGEKQNNVTI
                     AGLSAITFDAKKNIFYAINDSRNNNKEGDASLYTLKIQVSSRGIEQVNFLNQRPLLDA
                     KQQPFVTNTVDAEGLALTHNGKSLLWSSELGAPLRLSTLDGVMEKDFTSLFPARFNIS
                     SDKESSNGIRSGNAWEGLTVTPDGKSLFIAVESSLKQDGPIASPINSGTARLLQFSID
                     ADGRPSKQLHEYLYITDPVPQVSKFGINDNGVSEVLALNDHQLLVIERSGRNVSAGFN
                     DWDYSVRVYMVDLTAASDIKNIDSLQDWSNKSTLQPVSKKLLIDFADYTSSADCIEGV
                     TFGPLIDGHTSLIFVSDNNFQPH"
BASE COUNT         1436 a         1051 c         1122 g         1318 t
ORIGIN      
        1 aagcatactg cgcaataaaa ataaaagaat gtagagttgg tatcaaccac ttgaaatgag
       61 gctttgtcat gataagaatt tataaagcca gtaaaggtca gcttaagcat cgtgaaattt
      121 ttgatgaaat aaagaaaaag attgataacg gtgaatggaa agaaggcaaa gctataccaa
      181 cagaaagaga acttgctgaa cattttttaa gcagcagaac aacaattcgg aaagcaatag
      241 aaagtttgaa acaacgcgga tacctgcata gtgtccacgg gcaaggaaca tttacattac
      301 cggctcgcag tcgggaaaat catttattac atagttttac tgatgacatt aaggctcgtg
      361 gcggcgtacc tgcacagaat attcttgaaa taggttatat tcctctaagt gatattatca
      421 gaaaaaatct cgaacttgat atccatgtcc ataccgtttt ctgtataaaa cgcattcgct
      481 atatgggaag tacgccgctg ggtattcaga catcatggct tgcgttaaat gacggacaag
      541 aaataactca ggaagagctg ctggcgacgg gttccctcta tattttactg gaagaaaaac
      601 tcgctattaa acctttagaa gcaacggaaa ttatctctgc ccgacttccc tcgcctcttg
      661 agcgtcaatt actggaactg actgccgatg acgtcgtatt aagttgtacc cgcgtcacgt
      721 tatccgtgga acgcaaacca atggagtatg tagagatggt ttatcccgca agtcggtact
      781 cctacaaagc acgaattaca aaagacagtt ttagcgtgta acagagagaa tattgtgaac
      841 aaaatagaac aagccgtaat ttataacaaa atattaggtt ctttagcctg cgcgggtatg
      901 ggcgatgcat taggcgcagc gactgaactc tacagcattg atgaaataaa agcgcaatgg
      961 ggaggttttt taaatgcctt tgtttctcct cctgccgata cctttgctgg ctcattaaat
     1021 ggtattgcgg gacttattac cgacgattcc agccaaatgt atgtattttc agaagggtta
     1081 gttgaggctg gatttgataa cttcaccaat aatgactggt tagcttgtct tctgcgttgg
     1141 gccgatatgc agccctacgc aaattataaa ggccccacaa cagaacaaat cgtcaaggca
     1201 ctaaaagaag gtagaccaac aaacaccatt gggcgtattg gaacatcttc tcgtcaggca
     1261 cccaatgtcg gcaccacgaa cggcgcagga atgcgtgtag ccccggcagg attgatctgg
     1321 ccaggcaaga aagaaaaagc gtgtcacctg gcgctaataa cttgcctacc ttcgcatgac
     1381 accaatattg cgattgccag cgcttgcgcc atcgctgcgg cgacatcaca agctatgtta
     1441 ccagaagcct cgctcacatc attactggac gccgcaatct ggggagcgaa ttacggcgaa
     1501 cgcctggcca aacaatatgc acgatgtgtc gcagggccat ctatcgccat gcgcatccaa
     1561 cttgcagcgg acatcgccag acgagccaat gacctggagt cttgtctgcg agaaatggaa
     1621 ggtttggttg gtaactctgt tgccgcccat gaatccattc ctgcggcaat cggtctattg
     1681 ctgtactgca aaggagaacc ctgggaaacc attcatgcct gcgccaatat tggcaatgac
     1741 accgatagca tcgctacgat ggcaggcgca attgcagggg catggcgcgg ttttgatgcg
     1801 ctccctgaag ataaatatgc ctttttccgc gccgtaaata ataaagattt cgatattgaa
     1861 gcgatcgcat cgggtctgac attgcttgca ttacaggcac aggagaaata atgaacatgc
     1921 cagcccgtac tcctgttatg ccattagcgc aaactcgtga ggaaataaat gctacggcgg
     1981 catcgttacg ccatttggca gctatctggg gtgaatgttt ttctactttt cagctcgata
     2041 aacgtgctgt tgaacgcgtc tgtatagtgg gatcgggcga ctcctggacg gtggcactat
     2101 gtgccgccgc ctggctgggg aaatacacac atctgttttg ttacgcattg caaacgtggg
     2161 attttcttca gactgattta acccgttatc agaaagaaac gttaatcatt attctcagtg
     2221 caagtggcag accatcaatg accgttgacg ccctctgcca cgcagtatgc tcaaacgcgc
     2281 aagtgctggg tgtgacgaat ttccctggaa caccattttg cgcaataaca gaaaacatgc
     2341 tctatacatg ggcgaataag cagggtattc caacgcaaag tagtagcgtc acactttatg
     2401 ccctcctgcg cttagcgcaa aaattatgtc caggcttaac accactgcag attgaagagg
     2461 atttggaagg gaaatttagc cagattaatc agcactggca gcaaaagaag cgccattttt
     2521 accaacaaaa agagataacg tttcttggga gtggcttaag ttggggactg gcaatatcgg
     2581 ggagcaattt actttcttgc ggcccacaga ttcgtgcgac agcccttcca ctggaagaat
     2641 tttatcattc attacgctta catcaggctg gtgtcgggca gcattatatt ttactaccag
     2701 ccacatcctg cgagtcccca ttttaccttg caacccaaaa agcaattgtt gaacagggag
     2761 cgacagcaga attaatcagt tttatacctg acgccagtga gtccaacaat ttatttctgg
     2821 tcatgcagtg gttatatgaa atgtgctggc atttaagttg tgattatgtc gacgcaggcg
     2881 ggcaacgcgt tagccatagg gagaaataag actatgaaga aatttgatgt cgccgcttta
     2941 ggcagtggaa atatagatat ttttttatct atcccgtcac tccccactcg tggcggtaaa
     3001 gttgtcggga cacgtctggg tgaacaagct ggcggcacag tcgccaacag tgcctgtgcg
     3061 atgggacaac tgggtctcaa tgtggtatct gtttcatgtg tgggcaatga tcattcagcc
     3121 tcgatcattc ttgatggatt taaaaaatat catgtaaatt gtgatttcgt tcaggtaatt
     3181 ccggaattga ttgccaatac cgcgattatc tttattgatg aattaggtga aaaaacgttg
     3241 gtttactcgc ctggtagtga tcatgagtgg gataaagaaa aagccctgca ggcaattgcg
     3301 caaagccgtt atttttatac gatgccagca gatatcgaga aatttcgtat gctggccgaa
     3361 tattcccgta gccaaatgac aaaagttgtc gttgatattg agcctcatat cattgcaaca
     3421 cctgaacacc ttgccagaat tttacacctt gctgatattg cgatatttaa ttatgacggt
     3481 tttattcgtg gctatgcaac agagccagat tttacacttc tgcatgatat acaagacgag
     3541 tatcaacttg acgctgtcgt ggttacgctg gatgttaggg gagtaattgc agtcaaaggc
     3601 aatgagcaag taaaaatagg tagttataca attcccgtta ttgataccac aggcgctgga
     3661 gatactttta atggtgcatt tgtttatagc ttaataaaaa acatgccctt aattgaagca
     3721 ctaaaatttg cttctgccac agcagcaata aatattactg ccttaggtgc cagaggtcat
     3781 ctgccgacac catcggaggt taatcttttt ctttcacaac acgactaaaa tcatatatat
     3841 acaaaggtaa atatcatgaa aataagatat gccgctattt ttctggcatt accgctgtta
     3901 tgcacaacag cgcaggcaaa cagttttctt cattatgctg gggagtttaa cttccggacg
     3961 ggagaaaagc agaacaatgt cacaatcgcg gggctatcag ccatcacatt tgatgcaaaa
     4021 aaaaatattt tctatgctat taacgatagc agaaacaata ataaagaagg agatgccagt
     4081 ctttatacac taaaaataca ggtatcatcc aggggcattg agcaagtgaa ttttctcaac
     4141 cagcgtccac tgcttgatgc aaaacagcag cctttcgtga ccaatacggt ggatgccgaa
     4201 ggtctggccc tgacacacaa tggtaaatca ttactctgga gttcggagtt aggtgctcca
     4261 ctgcgtctga gcactctcga tggcgtaatg gagaaagatt ttacgtcttt atttcctgct
     4321 cgctttaata ttagttctga caaagagagc agcaatggta ttcgcagcgg caacgcctgg
     4381 gagggcctga cagttacacc agatggaaaa tcacttttta tcgccgttga aagcagcctt
     4441 aaacaggatg ggcctattgc aagtccaata aattcaggca cagcacgctt actgcaattt
     4501 tctatagatg cagacggtcg accttcaaag caattgcatg aatatctcta tattactgat
     4561 cctgttccgc aagtgagtaa gttcggtatt aatgataatg gcgtatccga ggtcctggcg
     4621 ctgaatgatc accaattgtt ggtcatcgaa cgttccggca gaaacgtcag tgctgggttc
     4681 aatgactggg attactctgt cagagtttat atggttgatt taactgctgc cagcgatatc
     4741 aagaatattg acagtctgca agattggtcc aataaatcca cgttacaacc cgtcagtaaa
     4801 aaattattaa ttgattttgc tgattacact tcctctgcgg attgtattga aggggtaacc
     4861 ttcggtcctc tgattgatgg gcatacatcg ttaatatttg tatctgataa taatttccaa
     4921 ccccatc
//
LOCUS       DAJOWB010000216         4895 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJOWB010000216 DAJOWB010000000
VERSION     DAJOWB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.217"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            135..2399
                     /locus_tag="OHO85_004645"
     CDS             135..2399
                     /locus_tag="OHO85_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HCQ2673891.1"
                     /translation="MSFIAIESNAVRKIVSERMLQSADYEKGQILADVLKEQKKGDFS
                     IDVKVVTTASGIYLLTKEPDKKRMLVFDTSNFNGFKRNSNEVITILQKSCRLAIKLWD
                     KIGHSPCEKIISGSSLIALLPFSFTTGKSYKVILDKAPDKERQEKRNESSFLIFSDGY
                     ETTNNTPVLSNFRKAKDAINSIDTENLFTSPKKDEEKSSYLRVNETDVFEKSSNPHMG
                     LANWIKKLTESQKKFVFSNSFGPDILKGAAGSGKTLSLILRCVVQLNKAQKEDKKLKA
                     IFITHSIATKDNIENLIASNGGVEYINSEGQQSVEVTTLQEWCIKNLGNRIETTEYLD
                     NDAFESKQLQLLYIDEAIDDFFINDYAGAVEFISPELKKFFNNDRWEISILLQEEIST
                     YIKGRAGEDMNIYMSLPRSKNVLPLKTEDDYKTIFSIFNKYQDKLISLNLFDSDDITL
                     SALKETSTPIWKRRRMSNGFDIMYIDETHLFNINELSLFHNLLKPNSNHIIFTMDRSQ
                     APGDNTITKDDVAKELDATLAHEHGLNAIFRSSEHIINLASCVLASGAALFQGLENPL
                     AESMSGHTSIVEERCNVPFIISKQNSHEVYKATFALADYMSENIGIQRSDVLIVPCTD
                     KILMELKEYALKASKEYISIEKRGDNIAVSKAAEDNLYIIGGMEYIGGLEFSAVIIIG
                     ADADKFPEKRNYDGESLHFINYSSFNKLYVAITRAKYQVAFVTEKTQKISPLLETACE
                     EGLIRYDDDLANIL"
     gene            2650..2766
                     /locus_tag="OHO85_004646"
     CDS             2650..2766
                     /locus_tag="OHO85_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2673892.1"
                     /translation="MRKARFTEHEIVTVIKSVEAGRTVKDICREAGISEPTY"
     gene            complement(3159..4504)
                     /locus_tag="OHO85_004647"
                     /pseudo
     CDS             complement(3159..4504)
                     /locus_tag="OHO85_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611902.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            complement(4740..>4895)
                     /locus_tag="OHO85_004648"
     CDS             complement(4740..>4895)
                     /locus_tag="OHO85_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001734924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673893.1"
                     /translation="SKERNLAAYLSRSSGIEALFQDVNALQKWLLQFGFISGGIATEK
                     MLITNKL"
BASE COUNT         1511 a          969 c         1014 g         1401 t
ORIGIN      
        1 attctgcagt atcgttggtc atttgagatc tccattcata gaacttttaa tgcagcgatt
       61 ttcgaataat tttgttcgca ttggtgtaga taatcacgac cataaactct taaccaatat
      121 tatggagaag tgacatgagt tttatcgcta ttgaaagtaa tgctgtacgt aagattgtgt
      181 cagaaagaat gctgcaatca gctgattatg aaaaaggtca aatacttgct gatgtattaa
      241 aggagcagaa aaaaggtgat ttcagtatag atgtgaaagt agtaactaca gcatcaggaa
      301 tttatttact gactaaagaa ccagataaaa aaagaatgct tgtttttgat acatctaatt
      361 tcaatggttt taaaagaaac agcaatgagg tcattaccat tcttcaaaaa tcatgtaggc
      421 tagctattaa attatgggat aagattggtc atagcccctg cgaaaaaata atcagcggct
      481 catcgctcat tgccttattg cccttttcgt ttacgacagg aaaatcgtat aaggtcattc
      541 tcgacaaagc gccagataaa gaacgtcagg aaaaaagaaa tgagagtagt ttcttgattt
      601 tttctgatgg ttatgaaacc actaataaca ctccggtatt atctaacttc cgcaaggcta
      661 aagacgcaat aaatagtatt gatacagaaa atctatttac cagtcctaaa aaagatgaag
      721 aaaaatcatc atatcttaga gtaaatgaaa ccgatgtatt tgaaaaatct tctaacccac
      781 acatgggact ggctaactgg attaaaaaac tgaccgaaag ccaaaaaaaa ttcgttttta
      841 gtaatagctt tggtcctgat attctcaaag gtgcagcagg ttcaggtaaa accctaagcc
      901 tgatattaag atgcgtagta caattaaata aagcccaaaa agaagataaa aaacttaaag
      961 ctattttcat aacgcacagt attgcaacaa aggataatat agaaaatcta atagcctcaa
     1021 atggtggagt tgaatatatt aatagtgaag gccaacagtc agttgaagtg acaactctac
     1081 aggaatggtg tattaaaaac cttggtaaca ggattgaaac aactgaatat cttgacaatg
     1141 atgctttcga atcaaaacaa cttcagcttt tgtatataga tgaggctatt gatgactttt
     1201 ttataaatga ttatgctggt gcagtagaat ttatctcccc agagttaaag aagtttttca
     1261 acaatgatcg ctgggaaata tcgatattac ttcaagaaga aatatcgacc tacattaaag
     1321 ggcgggctgg agaggatatg aatatatata tgtcactccc aagaagtaaa aacgtattac
     1381 ctttgaagac agaagatgat tataagacta tattttccat tttcaataaa taccaagata
     1441 aattaatttc attaaacctc tttgatagtg atgatatcac tttatctgca ctcaaagaaa
     1501 cctcaacgcc aatatggaaa aggaggcgaa tgagcaatgg atttgatata atgtacatag
     1561 atgaaacaca tctattcaac attaatgagc ttagtttgtt tcataactta ctaaaaccaa
     1621 actcaaatca cattatattt acaatggatc gcagtcaagc acctggtgac aacacaataa
     1681 ccaaagatga tgtcgctaaa gaattagatg caaccttggc tcatgaacac ggtttgaatg
     1741 ctatattccg ctcatctgaa cacattataa atctcgctag ttgtgtatta gcatcggggg
     1801 cggctctttt tcaagggctt gaaaacccct tagctgaatc aatgagtggt catacatcta
     1861 tcgttgaaga aagatgcaat gtaccattta ttatcagcaa gcaaaattca catgaggtat
     1921 ataaagctac atttgctcta gctgactata tgtctgaaaa tataggcatt caaagaagcg
     1981 atgttttaat agttccatgt acagataaaa tattaatgga gctaaaagaa tatgcattaa
     2041 aagcatcgaa agaatacatt agtatcgaaa aacgagggga taacattgct gtatcaaaag
     2101 cagcagaaga taacttatac atcattggtg gtatggagta tattggaggg cttgaatttt
     2161 ctgctgtcat aataatcggt gcagatgctg ataagtttcc agagaaaagg aattatgatg
     2221 gtgaatcact acactttatt aactattcat cattcaataa actttatgtt gcgattacaa
     2281 gggctaaata tcaggttgct tttgtgactg aaaaaaccca gaaaataagc ccattacttg
     2341 aaactgcatg cgaggaaggt ttgattagat atgatgatga tttagcgaac atcctgtaaa
     2401 gtcatatcta attatctgga taaatttaat taggtacatc cagtctgaaa atgcccgata
     2461 ataacacatc attgggcatt tgtccttttt gatatcgggg agatagatac tgtttctata
     2521 agaattagcc agggtgttca gagaatttca gatcgttggt aacttttaat tgtaagtatc
     2581 ccgcataatc gtgtcattca tatttagaga tcatccggca taatcaatct gccaatgaag
     2641 gagagcgcta tgcgtaaagc ccgttttact gagcatgaga tcgtcactgt gattaagtca
     2701 gttgaagccg gacgaaccgt taaagatatc tgccgggagg ccggtatctc tgaacccacc
     2761 tactagatct atggactacc tccgttttgc aagtactgaa tctggttttg agttgttgct
     2821 cacctctatc cggcatcagg aaaatctgtg cccaaatggg taatccacac acaatcgcct
     2881 caacaactgg acggcctctg aggccaatat aatagtcagg ttccgattga gcaggtgtaa
     2941 cctgtcacca cttctcagta cgctgcaact tttgctggca ggggattaat ttcacactac
     3001 ctgatactcg gtctcatttc tcagcatcga ccagattatt ctcgcgtttt tgttagcgac
     3061 cgccacggtc gtttgctgaa ctgagccgag aaatttctga aacaattagc aagcaatgtc
     3121 ctgactcttt tgtgtagttg aataaaaaag agcaggggtc agcgttttag gctggccctg
     3181 aattcctcgg gatttgccaa aaccgggtta attgccctga gttgctcctg aaccctggct
     3241 tgtgcgcgtc gtttaggcat ctttttgctc cgatcacgct taccatcgcg ttcgagttca
     3301 tccattttgc tttgcgccaa cttcagaaca gcaccaagac gtttgttatc aacaatttgt
     3361 ccctggtcga cacattccag tttgtcgaac acctgatatt tgagtgaatg gtaaccgtat
     3421 ttgaatgcca gcgtgccatc tggatagtcg tagactttta ttttttcgcc agcgatgcga
     3481 gtattttctt ctgtaggttc aaccagatat atcattttgt catagcggaa cgtcagcgtt
     3541 ttaggcagct tacgctgttc ctgccaggca aaaatatcat ccagctcatc gttgctttca
     3601 gccacggggc ggtgcagatc tttggggtat tttgctggtc ttgcgaagcg acgattgaag
     3661 tcagcgatga aggtatccag ccaggcattg gcctcctcaa tgctgctgat gccttcgagg
     3721 cgcatttcct tgataagcct gtcctgaagt gtctgatttg ctcgcttcac acgaccttta
     3781 gcctgtggac tgttggcaca aatcagcccg atccccagtt cagaaagtac gcggccaaat
     3841 tgcgtaacac cggtcgtggt ggaaccacca ttattgaccc tgaaaatgcc gtgcttatca
     3901 ctgtaaaacg ccaggggttt gccgtgttgc tcaagatact cacgagtggc catcatgtaa
     3961 tcaaaggctg attcagtctc accaaagcgc aggtgcatca ggcggctagt agcgtcatca
     4021 acaaagacga gcaggcagca tttcggcgca cgtccttcaa accagtcatg cggggaacca
     4081 tcaatctgaa caagttctcc gaggcagtca cgccggtatc tgggttgatg aacacgtggc
     4141 ttacgacgcg agtatgggac ccagagtcca tcagccgtca tccattttct gagcgtttca
     4201 acagacaccg tgatgttatg gcgttcgcgc aatttttctg cggcaagcgt tggtccaaag
     4261 tcgctgtagt tatcgtgaag taaagaaagt actcgcaact tcagtgactc agaaagtctg
     4321 tgattaccgg gcgtcctcgt cgaaggtttg ccagacctgc agcaccagat tcgcgaaagc
     4381 ggttcatcaa acgctgagtc tgacgttcgg tcagagcaag ttggtgtgca gcatcacgac
     4441 ggcgcatgcg cttttcgaca accgactgaa tgacattaat cctgttcagt tctttatctg
     4501 acatggtcac tagcatcctg atgtctccac ggcaataagg ttcaatcctt tatgccagaa
     4561 aaagtgacat ctctaaatgg gtagaaagtg acattagaat atgggagcta caatgtttgt
     4621 caaagctcag tgaaaatgtc taatcagtgg ctcgttagaa atgtccatat gttggaaagg
     4681 ccgctgcgag aatatctccc gaaccggcct ctttagcata acgtcattgt gcgaaccaat
     4741 tacaatttat ttgtaatcag cattttttca gtcgctatac caccactgat aaaaccaaat
     4801 tggagcaacc atttttgtaa tgcattcaca tcttggaaca gtgcttcgat accacttgat
     4861 cttgacaggt aagcagcaag gtttctttct ttact
//
LOCUS       DAJOWB010000217         4888 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJOWB010000217 DAJOWB010000000
VERSION     DAJOWB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.218"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            150..1124
                     /gene="hemB"
                     /locus_tag="OHO85_004649"
     CDS             150..1124
                     /gene="hemB"
                     /locus_tag="OHO85_004649"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCQ2673894.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1230..2081)
                     /gene="tauD"
                     /locus_tag="OHO85_004650"
     CDS             complement(1230..2081)
                     /gene="tauD"
                     /locus_tag="OHO85_004650"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCQ2673895.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2078..2905)
                     /gene="tauC"
                     /locus_tag="OHO85_004651"
     CDS             complement(2078..2905)
                     /gene="tauC"
                     /locus_tag="OHO85_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCQ2673896.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTLSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2902..3669)
                     /gene="tauB"
                     /locus_tag="OHO85_004652"
     CDS             complement(2902..3669)
                     /gene="tauB"
                     /locus_tag="OHO85_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCQ2673897.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSILLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLSLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3682..4644)
                     /gene="tauA"
                     /locus_tag="OHO85_004653"
     CDS             complement(3682..4644)
                     /gene="tauA"
                     /locus_tag="OHO85_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCQ2673898.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1134 a         1404 c         1243 g         1107 t
ORIGIN      
        1 gaatatgaaa ttattgctat ttctattaca aagatgtcct ggtctctctt tcattctctt
       61 cttgtcaaca tcacgacaac tttcgtaaaa cactcctgcc ccgcttcagg tatactgtgc
      121 accttaattc cacaaacatc aggcagacca tgacagactt aatccaacgc cctcgtcgcc
      181 tgcgcaaatc tcctgcgctg cgcgctatgt ttgaagagac aacacttagc cttaacgacc
      241 tggtgttgcc gatctttgtt gaagaagaaa ttgacgacta caaagccgtt gaagccatgc
      301 caggcgtgat gcgcattcca gagaaacatc tggcacgcga aattgaacgc atcgccaacg
      361 ccggtatccg ttccgtaatg acttttggca tctctcacca taccgatgaa accggcagcg
      421 acgcctggcg ggaagatgga ctggtggcgc gtatgtcgcg catctgcaag cagactgtgc
      481 cagaaatgat cgtcatgtca gacacttgtt tctgtgaata cacttctcac ggtcactgcg
      541 gtgtgctgtg cgagcatggc gtcgacaacg acgcgactct ggaaaattta ggcaagcaag
      601 ccgtggttgc agctgctgca ggcgcagact tcatcgcccc ttccgccgcg atggacggcc
      661 aggtacaggc gattcgtcag gcgctggacg ctgcaggctt taaagatacg gcgattatgt
      721 cgtattcgac caagttcgcc tcttcctttt atggcccgtt ccgtgaagct gccggaagcg
      781 cattaaaagg cgaccgcaaa agctatcaga tgaacccaat gaaccgtcgc gaggcgattc
      841 gtgagtcact gctggatgaa gcccagggcg cagactgcct gatggttaaa cctgctggag
      901 cgtacctcga catcgtgcgt gagctgcgtg aacgtactga attgccgatt ggcgcgtatc
      961 aggtgagcgg tgagtacgcg atgattaagt tcgccgcgct ggcgggtgct atagatgaag
     1021 agaaagtcgt gctcgaaagc ttaggttcaa ttaagcgtgc gggtgcggat ctgattttca
     1081 gctactttgc gctggatttg gctgagaaga agattctgcg ttaatttttt tcctctcaga
     1141 aagggggagt taacctgttg aaagtacggt gaacgccccc tcaccctaac cctctcccca
     1201 agggggcgag ggaaccgtcc gcactcgtat caccccgccc gataaaacgg tttatcccca
     1261 agaatcgtcg cccgatgcat aatccgccgc tgtggcaggt aatcggcatt ggcatagtgc
     1321 tgagtcacgc ggttatccca aatcgcaata tcattcggtt gccagcgcca acgcacctga
     1381 aactccggtt tagtgatatg ggcgaataaa aagcctaaca aggcttcgct ctctttctcg
     1441 ctgacatcga caattcgcgt agtaaagcct tcattcacaa acagcgcctg tttaccgctc
     1501 accggatgcg ttcgcaccac cggatgtagc aacggaggat ttttcgcgac cgcctcgcgc
     1561 cagcgttgat gttcctcctc ggttttgcgg tatttgtatt ccgggaacga tttacggaaa
     1621 tcatgctccg cacgcagccc gctcagcagc tggcggaagg gaacagagag cgcctcatag
     1681 gccgcaatac cgctggtcca gagcgtatca ccgccggtcg aaggtaattc tttagctgcc
     1741 agaatcgccc ctgcgggtgg cgtttcaata aatgtcacat cggtatgcca gttgtcgtta
     1801 tctggcggat tatcgttatg ggtatccagc acgatgatct cgtcaacccc ttcggcatga
     1861 gggtaaacag ggtgaatatg cagttcgcca aaacgctggg ccagcgcgcg ttgctgctgc
     1921 ggcgtaatgg cttgatcgcg cagaaacacc acctgatggc gcagcaccgc atggtaaagc
     1981 tgttcaaact gattatcgct taacgggcgc gtcaggtcgg cacccgaaat ttgtgcgccg
     2041 atatacggcc ccagcggggt aatgctcaga cgttcactca ttgtacttct ccatgccagg
     2101 gcgtcagacg gcgctgtaac gcgcgcaaac ccagttctaa aagaaaggcg ataatcgcaa
     2161 tcaccgcgat tcccgccagc accacgtcag tcgcgagaaa ttcacccgct gactgaacca
     2221 taaatcctaa accgcgcgtc gcggcaatca gctccgccgc taccagcgta gaccagccca
     2281 cacccagacc aatgcgtaat ccggtgagga tttccggcag cgcgccgggc aaaatgacaa
     2341 accacagcac ctgcgcacgg ctggcaccca gcgactgggc ggcacgaatg cgaacctgct
     2401 gcgcgctttt cacccccgcc agcgccgaca tcgccaccgg tgcaaaaatc gctaaataga
     2461 tcagtaagat cttcgaggtt tcaccaatac caaaccagat caccatcagc ggcaaataag
     2521 ccagcggcgg caccggacga taaagttcga ttaccggatc cagaatgccg cgcaccgtcg
     2581 ggcttagccc catggcgatc ccgaccggaa taccgatcaa caccgccgcg agtagcgcca
     2641 gcacaatgcg cgtcaggctg gctgccagat gctgccacag cgtggcgtcc ataaatccct
     2701 gcggcccggc aatggtgagc agttttgcca gtacctgttg cggtggcggc aaaaacagcg
     2761 ggctaatcag ttgcagcgct gcgactgccc accatacagt aagtaaaacc gctaacgtgc
     2821 caatgcttaa ggtcacctga cgcgagagcg gccagcgcca tttaagccga tgcgaatgca
     2881 gtttttcatt aatgagcaca ctcatgagaa cgcctcccgc tgctcaaata cgcggcttaa
     2941 aacatattcg cgcatggcga tgaattgtgg atcggacttg atgctgcggc tcgactctcc
     3001 cgcaacaaag cggcgagcaa agttgagcga cagccgctcc agcacacggc cagggccgga
     3061 tgaaagcaga accagttcag tcgccataaa caccgcttct tctatatcgt gggtaatcaa
     3121 cagcacctgc ttgcccgtct cctgccagag tttcagcagc agggtttgca tctggtcgcg
     3181 ggtgaaggcg tccagcgcac caaacggttc gtcgagtaat aacagctggg gattcgccgc
     3241 cagcgcacgg gcaatcccca cccgctgacg ttgaccaccg gaaagctgcc agatgtagcg
     3301 tttttctgcg ccttccagcc ccactttttt cagcatctgg tgcgcgattt ccagtcgctg
     3361 cattttctct atacctgcca attgcaggcc gaacgccacg ttgtcctgta cattgcgcca
     3421 cggtagtagc ccttcattct gaaaaactac gccacgctct gctcccggtc cctcaatacg
     3481 cttacccgcc agtagaatgc tgccatgctg ataaggcaca aaaccggcaa tcagattcag
     3541 caacgtggtt ttaccgcagc cggacggccc cagcaccacc agtagctcgc cgctttccag
     3601 cgtcaggttg atatcttcca gtgccggttt gccgccataa tcggcgtaaa gatgagagat
     3661 ttgcagcatc tgcgcctcct tttattgcac gaagcgcgag gtaacgtact ggctgtaatc
     3721 attcgctaca gccgggacct tgccctgctc tttcaaaaac tgcgcggtgt cgatgatcgc
     3781 tttgttcacc ggtccggtca gttctgccgt ttgttgctgc ggcgtcagat aggtattccc
     3841 cttcaccagc cccggaacgt caccttcagg cacgccgctt aaacgcgcca gtttgctgat
     3901 gttttccggc tgtttcagcc acacgtctgg gttagcaatg tacggttgct gagcatcgat
     3961 ggcgctttta gcgaacgctt tcacgacctc aggatgtttc tcggcaaaat ctttgcgcac
     4021 cacccagacg tccagcgttg gcgcgcccca ctgcccgacc tgttcagaat cggtcaacac
     4081 cttgccgtct ttttccaggg cgttaaccgc cggtgcccag acataagcac catcaatatc
     4141 tccccgctgc caggcagcga taatcgcggg cggctgcagg ttcacaatct ccacttgccc
     4201 gggtttaatg ccccagtgtt tcagcgccgc cagcaggctg tagtgggtgg tggagataaa
     4261 cggtacggcg atgcgtttgc caatcagatc ttccggtttg ctgatagttt tctttaccac
     4321 cagcgcttcg gagttaccca gttttgacgc cagcaagaag acttcaatcg gcacctgttg
     4381 gctggctgca accgctaacg ggctggaacc gaggttgccg atttgcacat cgcctgaagc
     4441 cagcgcccgc acgatgctgg ctccgctgtc aaacttacgc cagtccacgc tcgccccgct
     4501 ctctttagca aaggtgttgt cggcctgagc cactttcgcc ggttcggctg aggtttgata
     4561 ggccacggtg acgttcaccg cctgtgcctg aaaagcgatg aatgccagtg cggctagaag
     4621 tgtgttacgc gatgaaattg ccatgattgt ctgctcccct gtcttgttat gggagcagta
     4681 ttcaggaata aaaacattca ttaaaagaat tagtcgttat cgcacagatg attttattct
     4741 tagcaaaaaa acggctaagg aaagattaaa tatgcaatta ctccgttcta acaattggga
     4801 aaattactac acttcatttt agtgatgcat aaacaaccac attacgagat ttattacttc
     4861 attatttcta tcaatatcaa tacatata
//
LOCUS       DAJOWB010000218         4874 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJOWB010000218 DAJOWB010000000
VERSION     DAJOWB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.219"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            595..1092
                     /locus_tag="OHO85_004654"
     CDS             595..1092
                     /locus_tag="OHO85_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCQ2673899.1"
                     /translation="MGKDPEVRYIPNGGAVANLQVATSETWRDKQTGEMREQTEWHRV
                     VLFGKLAEVAGEYLRKGVQVYIEGQLRTRSWEDNGITRYVTEILVKTTGTMQMLGRAA
                     GAQTQLEEGQQFSGQPQPEPQAEAGTKKGGAKTKGRGRKAAQPEPQLQPPEGDDYGFS
                     DDIPF"
     gene            1155..1388
                     /locus_tag="OHO85_004655"
     CDS             1155..1388
                     /locus_tag="OHO85_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HCQ2673900.1"
                     /translation="MREYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDHGEHFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHAIRDFGILK"
     gene            1447..3405
                     /locus_tag="OHO85_004656"
                     /pseudo
     CDS             1447..3405
                     /locus_tag="OHO85_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
     gene            3460..3894
                     /gene="psiB"
                     /locus_tag="OHO85_004657"
     CDS             3460..3894
                     /gene="psiB"
                     /locus_tag="OHO85_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HCQ2673901.1"
                     /translation="MKTELTLNVLQTMNAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFVPEAVSHSLALAASLDEQGYSVSDIIHILMAEGGQA"
     gene            3891..4610
                     /locus_tag="OHO85_004658"
     CDS             3891..4610
                     /locus_tag="OHO85_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HCQ2673902.1"
                     /translation="MSACSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARE
                     ASHAVREMERWMVPNKLREAA"
BASE COUNT         1159 a         1263 c         1523 g          929 t
ORIGIN      
        1 caaatcgcta cggcaaaata atggagatct ggctattacc gttcaggcca aaaatggatc
       61 ccgaaagatt gaggttttca aactcaaaca tgtcaatcgc aaccgaacaa cgattgtcga
      121 tacggagccg agaaccgcac atatcacaac ccgcacaaca gagatcatgc gtcgattagg
      181 ggcaaggatc tgcgagtatt gtgcgaagac tgggcgctgc gaagtacatc atgtcagaaa
      241 gctaaaagac ctgaagaaag gtcgtaaggc cggatataaa cccagcctgt ggcaactaat
      301 gatgatcgca cgccgccgga aaacgatgat tttgtgcgca agttgccacg acaagttaca
      361 tagcggaaaa ctacccgatc tgagacagga aaagggaaga atactcctgc aaccacctgt
      421 ctcaagcggg gattctgcta aatgagttta tccccaataa gctagcaatg gagagccgta
      481 tgcattgaaa ggtgcacgta cggttcggag ggggggtaac ggtgatctaa ctcaagataa
      541 gcaggacata ttcgccattt atcttgaaag accacacgta ccctacccta ccgcctggga
      601 aaagatccgg aagtccgtta catccccaac gggggcgcag tggcaaacct gcaggtcgcc
      661 acgtcggaaa cctggcgtga caaacagaca ggggagatgc gggagcagac agaatggcat
      721 cgcgtggtgc tgttcggcaa gctcgcggaa gtggctggcg aatatctgcg caagggcgtg
      781 caggtctaca tcgaaggtca gcttcgcacc cgtagctggg aagataacgg tatcacccgt
      841 tacgtcaccg aaattcttgt taagaccaca ggcaccatgc agatgctggg acgtgccgca
      901 ggtgctcaga ctcagctgga agaggggcaa cagttcagcg gtcagcctca gccggaacca
      961 caggcggagg ccggtacgaa aaaaggtggc gcaaaaacga aaggccgtgg acgtaaggcc
     1021 gcgcagccgg agcctcagct gcaaccgccg gagggtgacg attacgggtt ttcagacgat
     1081 atcccgttct gaacgggctg actgtgataa ccgccccgcc ccgttctgtg cggggcatca
     1141 ccggagagat gaggatgaga gaatatttca gaatacttca ggggctgccg gacggctcct
     1201 ttacccgcga acaggcggaa gccgttgccg cacagtaccg gaacgtcttt atcgaggatg
     1261 accacgggga acattttcgc ctggttgtcc gcaataacgg tgcaatggtc tggcgtacct
     1321 ggaattttga ggacggtgcc gggtactgga tgaaccatgc catcagggat ttcgggattc
     1381 ttaagtaaga gaggtgccgg acgcgcgaac gtccggcagg acataagcaa ttataagggg
     1441 atgattatgc ctgtaacgaa gtgtgaacca gaaaccaccc gcaaagcaag ccgtaaatat
     1501 gcaaaaacgc aggaaacagt cctgtctgcc ctgctggcgc agacggagga agtgagcgtg
     1561 ccgctggcct cactgattaa atcaccgctg aatgtgcgca cggtgccgta ttctgcggag
     1621 tccgtcagcg aactggcgga ctccattaaa tgtgtcggcc tgttacagaa tctggttgtt
     1681 catgccctgc caggtgaccg ttacggtgtc gccgcaggcg gtcgccgact ggcagcactc
     1741 aacatgctgg cagagcgcaa catccttccg gctgactggc ctgtacgcgt gaaggtcatt
     1801 ccgcaggagc tggcgactgc cgcatcgatg accgagaacg gtcagcgtcg ggatatgcac
     1861 cctgccgaac agattgccgg attccgcgca atggcgtagg aaggcaaaac acctgcgcag
     1921 actggcgacc tgctgggcta ttcaccccgc cacgtccagc gaatgctgaa actggcagac
     1981 cttgcacccg tcatcctcga tgcgctggca gaagaccgca tcaccacaga gcactgtcag
     2041 gcgctggcgc tggagaacga caccgcgcgt caggtacagg tgtttgaagc cgcctgtcag
     2101 tcgggatggg gcggaaaacc ggaagtacag accattcgtc gtctggtgac cgaaagtgaa
     2161 gtggcggtgg cggggaacag taaattccgc ttcgtggggg ctgatgcctt ctcgccagac
     2221 gaactgcgca ccgatttgtt cagcgatgac ggggacggtt atgtcgaccg cgtggcgctc
     2281 gatgccgccc tgctggaaaa actccaggct gtcgctgaac accttcggga agccgaaggc
     2341 tgggaatggt gcgccggacg catggagcct gtcggtttct gccgtgagga tgccggaaca
     2401 taccgcagtc tgccggagcc ggaagcggtg ctgacggagg cagaagaaga acgcctgaac
     2461 gaactgatgg cacgttacga tgcgctggaa aaccagtgtg aggaatccga cctgctggaa
     2521 gccgaaatga agctgatgcg ctgcatggcg aaggtcaggg cgtggacgcc ggagatgcgt
     2581 gccggaagtg gcgtggtggt gtcctggcgt tatggcaacg tgtgtgtcca gcgtggtgtg
     2641 cagttgcgca gtgaggatga tgtggctgat aacgattacc gcacggaaca ggtgcaggag
     2701 aaagcctcag tggaggaaat cagtctgccg ctgctgacga aaatgtcctc agagcgcacg
     2761 ctggcagtcc aggcggcact gatgcagcaa tcagacaaat ccctggcact tctggcatgg
     2821 acactctgtc tgaatgtatt tggcagtgga gcgtacagca atcccgccag aatccgcctg
     2881 gaatgtgagc attattcgct gaccagcgat gcgccgtcag ggaaggaagg tgccgcattc
     2941 atggcgatga tggcagaaaa agcccgtctt gccgccctgc tgccggatgg atgggcacgg
     3001 gatatgacga cgttcctgtc actcagtcag gaggtgctgt tatcactgct cagtttctgc
     3061 accgcgtgca gtatccacgg tgtccagacc cgtgagtatg gtcacacgtc acgcagtccg
     3121 ctggacacgc tggagagcgc catcggcttt cacatgcgcg actggtggca gccgacgaaa
     3181 gcaaactttt tcggacacct gaaaaagccg cagattatcg cagccctgaa tgaggcagga
     3241 ctatccggtg ccgcacggga cgcggagaag atgaagaaag gcgatgcggc tgaacatgca
     3301 gagcaccata tgaaagacaa ccgctgggtg cctggctgga tgtgtgcacc acgcccacag
     3361 acggatgcca ctgaacgcac cgataacctg gctgatgccg cctgatgaac aaccacaccg
     3421 ccccgccgga gacggggcgg cagcaaggga gataccgtga tgaaaactga actgaccctg
     3481 aatgtattac agaccatgaa cgcacaggaa tatgaagata tccgggctgc cggaagtgat
     3541 gaacgccgtg agctgactca cgccgtgatg cgggagctgg atgcgccgga taactggacg
     3601 atgaacggcg agtacggcag cgagtttggc ggctttttcc ccgtccaggt ccgtttcacg
     3661 cctgcccacg aacgttttca cctggcgtta tgttcaccag gtgacgtctc tcaggtctgg
     3721 gtgctggttc tggtcaatgc cggtggtgag ccgtttgcgg tggttcaggt gcagcggcgg
     3781 tttgtaccgg aagccgtcag ccattcactg gcactggcgg cgtcactgga tgaacaggga
     3841 tacagcgtca gtgacatcat ccatatcctg atggcggaag gaggtcaggc atgagcgcat
     3901 gttcacaggc actggttccc ctcagcacag agcaacaggc cgcatggcgg gcggtggcgg
     3961 agacggaaaa acgccgccat cagggaaaca cgctggcgga atatccgtat gcgggggcgt
     4021 ttttccgctg tctgaacgga agccgcagga tttcgctgtc tgacctgcga tttttcatgc
     4081 cgtccctgac cgcagaagaa ctgcacggca accgtcttca gtggctgtat gccatcgatg
     4141 tgctgattga aacacagggc gaggtctgcc tccttcccct tcccggtgat gcagcagagc
     4201 gcttgttccc gtcggtgcgg ttccgtgtca gggagcgcag tcgccacaaa tccgcactgg
     4261 tcatgcagaa gtacagccgt cagcaggcac gggaggcaga gcagaaagcc cgtgcgtacc
     4321 aagcactggt tgctcaggct gaaatcgaac tggcgttcca ttcaccggaa acagtgggaa
     4381 gctggcatgc acgctggagt gaccgggtgg cagagcatga cctggaaacc ctgttctggc
     4441 agtggggcga gcgttttccg tcactggccg gaatggaacg ctggcagtgg caggatatgc
     4501 cattctggca ggttatcgca gaggctggta tggcggcaag ggaggccagt catgctgtcc
     4561 gtgagatgga gcggtggatg gtgccgaaca aactccggga ggcggcgtga tgcgcaacaa
     4621 tcacacggat ttcccgtgaa cggtctgaat gagcggattc ttttcaggaa aagtgagtgt
     4681 ggtcagcgtg cagggatatg agctatgatg tgcccggcgc ttgaggcttt ctgcctcatg
     4741 acgtgaaggt ggtttgttac cgtgttgtgt ggcagaaggc agaaagcccc gtagttaatt
     4801 tttcattaac ccacgaggcc ccctgtatgt ctgatcaaca acagtatggc ctcttaccgt
     4861 gcttaatgca agga
//
LOCUS       DAJOWB010000219         4862 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJOWB010000219 DAJOWB010000000
VERSION     DAJOWB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.220"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..353)
                     /locus_tag="OHO85_004659"
     CDS             complement(<1..353)
                     /locus_tag="OHO85_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HCQ2673903.1"
                     /translation="MKKVIVVSALAMAGVFSAQALADRGKTGFYVTGKAGASVVTQTD
                     QRFRQDFGDDVYKYKGGDKNDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGAADSHY
                     TLDTWHSPMGDGGRE"
     gene            complement(713..1723)
                     /gene="papG-II"
                     /locus_tag="OHO85_004660"
     CDS             complement(713..1723)
                     /gene="papG-II"
                     /locus_tag="OHO85_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151505562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbria tip G-adhesin PapG-II"
                     /protein_id="HCQ2673904.1"
                     /translation="MKKWFPALLFSLCVSGESSAWNNIVFYSLGNVNSYQGGNVVITQ
                     RPQFITSWRPGIATVTWNQCNGPGFADGSWAYYREYIAWVVFPKKVMTKNGYPLFIEV
                     HNKGSWSEENTGDNDSYFFLKGYKWDERAFDAGNLCQKPGETTRLTEKFDDIIFKVAL
                     PADLPLGDYSVTIPYTSGMQRHFASYLGARFKIPYNVAKTLPRENEMLFLFKNIGGCR
                     PSAQSLEIKHGDLSINSANNHYAAQTLSVSCDVPANIRFMLLRNTTPTYSHGKKFSVG
                     LGHGWDSIVSVNGVDTGETTMRWYKAGTQNLTIGSRLYGESSKIQPGVLSGSATLLMI
                     LP"
     gene            complement(1767..2267)
                     /gene="papF"
                     /locus_tag="OHO85_004661"
     CDS             complement(1767..2267)
                     /gene="papF"
                     /locus_tag="OHO85_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042092232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial tip protein PapF"
                     /protein_id="HCQ2673905.1"
                     /translation="MIRLSLFISLLLTSVAVLADVQINIRGNVYIPPCTINNGQNIVV
                     DFGNINPEHVDNSRGEITKTISISCTYKSGSPWIKVTGNAMAGQTNVLATNIANFGIA
                     LYQGKGMSTPLTLGNGSGNGYRVTAGLDTARSTFTFTSVPFRNGSRTLNGGDFRTTAS
                     MSMIYN"
     gene            complement(2342..2863)
                     /gene="papE"
                     /locus_tag="OHO85_004662"
     CDS             complement(2342..2863)
                     /gene="papE"
                     /locus_tag="OHO85_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial minor subunit PapE"
                     /protein_id="HCQ2673906.1"
                     /translation="MKKIRGLCLPVMLGAVLMSQHVHAVDNLTFRGKLIIPACTVSNT
                     TVDWQDVEIQTLSQNGNHEKEFTVNMRCPYNLGTMKVTITATNTYNNAILVQNTSNTS
                     SDGLLVYLYNSNAGNIGTAITLGTPFTPGKITGNNADKTISLHAKLGYKGNMQNLIAG
                     PFSATATLVASYS"
     gene            complement(2890..3426)
                     /locus_tag="OHO85_004663"
     CDS             complement(2890..3426)
                     /locus_tag="OHO85_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCQ2673907.1"
                     /translation="MIKSTGALLLFAALSAGQAIASDVAFRGNLLDRPCHVSGDSLNK
                     HVVFKTRASRDFWYPPGRSPTESFVIRLENCHATAVGKIVTLTFKGTEEAALPGHLKV
                     TGVNAGRLGIALLDTDGSSLLKPGASHNKGQGEKVTGNSLELPFGAYVVATPEALRTK
                     SVVPGDYEATATFELTYR"
     gene            complement(3436..4017)
                     /locus_tag="OHO85_004664"
     CDS             complement(3436..4017)
                     /locus_tag="OHO85_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein papJ"
                     /protein_id="HCQ2673908.1"
                     /translation="MVVNKTTAVLYLIALSLSGFIHTFLRAEERGIYDDVFTADELHH
                     YRINERGGRTGSLAVSGALLSSPCTLVSNEVPLSLRPENHSASAGAPLMLRLAGCGDG
                     GALQPGKRGVAMTVSGSLVTGPGTGSALLPDRKLSGCDHLVIHDGDTFLLCRPDRRQE
                     EMLAAWRKRATQEGEYSDARSNPAMLRLSIKYE"
     gene            complement(4054..4788)
                     /locus_tag="OHO85_004665"
     CDS             complement(4054..4788)
                     /locus_tag="OHO85_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCQ2673909.1"
                     /translation="MWKLSMIRKKILMAAIPLFVISGADAAVSLDRTRAVFDGSEKSM
                     TLDISNDNKQLPYLAQAWIENENQEKIITGPVIATPPVQRLEPGAKSMVRLSTTPDIS
                     KLPQDRESLFYFNLREIPPRSEKANVLQIALQTKIKLFYRPAAIKTRPNEVWQDQLIL
                     NKVSGGYRIENPTPYYVTVIGLGGSEKQAEEGEFETVMLSPRSEQTVKSANYNTPYLS
                     YINDYGGRPVLSFICNGSRCSVKKEK"
BASE COUNT         1317 a         1225 c         1012 g         1308 t
ORIGIN      
        1 tcttcccgac caccatcccc catcggagaa tgccatgtat ccagtgtata atgggagtct
       61 gcagctccgc ggccatagaa ttccacttcc gtgcgcacag gaacattgta atgttgataa
      121 aaatcatagc ccaccgcaag gcctgcacca aatacagtat cgtttttatc accgccctta
      181 tacttataaa catcatcccc aaagtcctga cggaagcgct ggtcagtctg agtcacaacc
      241 gaagcaccag ctttaccggt cacataaaag cctgttttac ccctatcagc cagagcctgg
      301 gctgaaaata cgcccgccat agcaagcgct gaaacaacaa taaccttttt catttcaatc
      361 atctccgttg gttcttaccc gcacgataaa gaaggttgct tattttaaaa ccatgcaaaa
      421 gacagggtaa aaagagaaaa ttcgatcgaa tcgatcgaca aaatcgatca tgcatgatga
      481 agatttctta tcgaagacat gaaaatggta aggaatggtt taagaggtaa ttgcaacggc
      541 atggaagtat tgagaccaga acagcacgag ataaagcggc tgaacgtcag atgagctaca
      601 tggacgtaac tcagtgcagg ccgcgtataa tccataaatg aagtggctga aaggtgactt
      661 cccggggcat cccagatatc cacaacactc tatccgtctc cggataaacc atttatggca
      721 atatcatgag cagcgttgct gaaccagata gtactcctgg ttgtatcttt gaagattcac
      781 catagaggcg actgccgatg gtcaggtttt gtgtacctgc tttgtaccat ctcatcgttg
      841 tctctcctgt gtccaccccg ttaaccgaaa caatggagtc ccagccatgc cccagaccaa
      901 ccgaaaattt cttaccatgg ctgtatgtcg gagttgtatt tcttaacagc ataaaacgaa
      961 tatttgcagg cacatcgcaa gacacagaaa gagtctgagc cgcataatga ttattagcgc
     1021 tattaataga cagatcacca tgctttattt ccagagactg tgcagaagga cggcatccgc
     1081 cgatattctt aaataagaat aacatttcat tctctcttgg gagagttttg gccacattgt
     1141 atgggatttt aaaacgggct cccaagtaac tcgcgaaatg acgctgcatg ccggaagtgt
     1201 atggaattgt aacagaataa tcccctaaag gaagatctgc aggtagggcg actttaaaaa
     1261 taatatcgtc aaatttctca gtcagacggg ttgtttctcc tggtttctga cacaaattac
     1321 ctgcatcaaa ggcccgctca tcccacttat accccttgag aaaaaaatag ctgtcattgt
     1381 caccagtatt ctcctcactc cagctacctt tattatgaac ctcaataaat aagggatatc
     1441 catttttggt cataaccttt ttggggaata ctacccacgc aatatactcc ctgtagtaag
     1501 cccaggaacc atcagcaaac ccaggaccat tacactgatt ccaggttacc gtagcaatgc
     1561 ccgggcgcca cgaagttata aattgtggcc tttgagtaat caccacattc cctccctgat
     1621 aagagttaac gtttccaagg gagtaaaaga caatattatt ccatgcagag gactcaccag
     1681 acacacacaa ggaaaataac aaagctggga accatttttt catcagaaat cacttccgta
     1741 attacagtta ttcatttagg tatgactcag ttataaatca tgctcatact ggccgtggtc
     1801 cggaaatccc cgccattcag tgtcctgctg ccattacgaa agggcactga agtaaaggtg
     1861 aacgttgaac gtgctgtgtc cagacctgct gtcactctgt aaccatttcc tgaaccatta
     1921 cctaatgtaa gaggtgttga cattcctttt ccctgataca acgctatacc aaaattggct
     1981 atatttgttg ccagtacatt agtctgccca gccattgcat tacctgtgac ctttatccag
     2041 ggtgagccac tcttatacgt acaggatatg cttatggttt ttgtgatttc accacgtgag
     2101 ttgtccacgt gctcaggatt aatattccca aaatcgacaa caatattctg cccgttatta
     2161 atggtgcatg gggggatata aacatttccc ctgatgttaa tctgcacatc agccagtaca
     2221 gcgaccgatg tcagaagcaa cgatataaat aatgataaac gaatcatccc ccctccggag
     2281 agcggcacag aaaacatttt attttacgag atataaaatt aacgtatttt agttgatact
     2341 attacgaata tgatgcaacc agcgttgctg ttgcagagaa aggaccggct atcaaattct
     2401 gcatattccc tttatatcca agtttggcat gaagtgatat agttttatct gcattattac
     2461 ctgtgatttt tccgggcgta aatggagtcc ctaaagttat cgcagtccca atatttcctg
     2521 cattactgtt ataaagataa acgagtaacc catcagaaga tgtgtttgat gtattctgaa
     2581 ctaaaatagc attgttataa gtgtttgttg ccgttatcgt aaccttcatt gttcccagat
     2641 tatagggaca ccgcatattc acagtaaact ctttttcgtg atttccattt tgactcaggg
     2701 tctgaatctc tacatcctgc cagtcaacag ttgtgttgct tacagtacag gcaggaataa
     2761 tcagttttcc tctgaaggtc agattatcaa ctgcatgtac atgctgagac attaacactg
     2821 cccccagcat taccggaaga cacaaacctc ttatcttttt catctgaaat atcctgtaca
     2881 aaaattttgc taacgatatg tcaattcaaa cgtggctgtt gcttcataat caccgggtac
     2941 cacactcttc gtccgcaggg cttccggcgt tgccacaaca tacgcgccga aaggaagctc
     3001 aagactgttt ccggtaacct tttccccctg gcctttgtta tgggaagcgc cgggtttcag
     3061 cagactgctg ccatcggtgt ccagcagtgc aatgcctaac cggccggcat tcactccggt
     3121 taccttcaga tggcccggga gggccgcctc ttccgttccc ttaaaggtca gggtcacaat
     3181 tttgccaact gctgttgcat ggcagttttc cagcctgatg acaaacgact ctgtcggcga
     3241 acgtccgggc ggataccaga aatccctgga cgcccgggtt ttgaagacga catgtttatt
     3301 cagactgtca ccggacacat ggcagggtct gtcaagcaga ttacccctga atgccacatc
     3361 tgaggctatt gcctgtccgg cagacagtgc ggcaaacagt aaaagagcgc ctgtgctttt
     3421 tatcatcaca ttcccttact catattttat gctcagacgc agcatggccg gattgctcct
     3481 ggcatcagaa tactcaccct cctgtgtcgc ccttttcctc caggcggcca gcatctcctc
     3541 ctgccgccgg tcaggccggc acagtaaaaa ggtatcacca tcgtgtataa caagatggtc
     3601 acagccggat agcttacggt caggaagtaa agcacttccg gttccgggac cggttaccag
     3661 tgagccggag actgtcatcg caacgccccg ttttccgggc tgaagtgcac caccgtcccc
     3721 acatcctgcc agcctcagca tcagaggtgc tccggctgac gcagagtgat tctccggccg
     3781 gaggcttaac ggcacctcat tactcaccag cgtgcagggg gaggacagca gtgcaccact
     3841 gacggccagg cttccggtgc gccccccccg ttcatttatc cggtaatgat gcaactcatc
     3901 tgcagtaaag acgtcatcgt atataccccg ctcttcagcc cgcaggaaag tatggatgaa
     3961 accactcagc gacagtgcaa taagatacag tactgctgtt gttttattca caaccataat
     4021 atcccacccg catttaaccg ttattgcggt acattatttc tcttttttca cagagcaacg
     4081 gctgccatta cagataaacg acagtaccgg gcgaccacca tagtcgttaa tataagacag
     4141 ataaggggta ttataatttg ccgattttac tgtctgctct gaacggggag acagcatcac
     4201 ggtttcaaac tcaccttcct ctgcctgctt ttcacttcct cccagaccaa taacagtgac
     4261 ataatagggc gttgggtttt caatacgata cccaccgctg actttgttca gaattaactg
     4321 gtcctgccat acttcatttg gtctggtttt aattgctgcc gggcgataaa aaagctttat
     4381 tttggtctgt aaggctatct gcagtacatt ggccttttca ctcctcggcg gtatttcccg
     4441 gagattaaaa taaaacagtg attccctgtc ctgaggaagt ttactgatat ccggtgtggt
     4501 actcagcctg accatgcttt tcgcacccgg ctcaaggcgc tgaaccggag gggtggcaat
     4561 aaccggccct gtaataattt tttcctgatt ttcattttct atccatgcct gagcaagata
     4621 gggcagttgt ttgttatcat tggagatatc aagcgtcatt gacttctcac tcccgtcaaa
     4681 caccgcgcgg gttctgtcca gcgaaacagc agcgtctgcc ccggatataa caaacagggg
     4741 gatggcagcc atcagaatct tttttcgaat catacttaat ttccacattc tgtaatttca
     4801 cctggtccgg aaaatggcat aaccgcattc atcagccgtt aatattccgg actttcattt
     4861 at
//
LOCUS       DAJOWB010000220         4803 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJOWB010000220 DAJOWB010000000
VERSION     DAJOWB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.221"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            37..258
                     /gene="traR"
                     /locus_tag="OHO85_004666"
     CDS             37..258
                     /gene="traR"
                     /locus_tag="OHO85_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HCQ2673910.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PESRRKIFPGVTLCVECQAYQERQRKHYA"
     gene            418..3045
                     /gene="traC"
                     /locus_tag="OHO85_004667"
     CDS             418..3045
                     /gene="traC"
                     /locus_tag="OHO85_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HCQ2673911.1"
                     /translation="MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLL
                     PYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLMS
                     SQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLALTLRHYRV
                     FISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVGEMINHNPD
                     SLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPE
                     IAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSY
                     AKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALKVEQDILNSFR
                     KNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNP
                     LTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGG
                     FAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNG
                     NLDEVHEGLLLQAVRASWLAKKNQARIDDVVNFLKNASDSEQYAGSPTIRSRLDEMIV
                     LLDQYTANGTYGRYFNSDEPSLRDDARMVVLELGGLEDRPSLLVAVMFSLIIYIENRM
                     YRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSD
                     KASSAARAAWGNSSYKIILRQSAKEFAKYNQLYPDQFQPLQRDMIGKFGAAKDQWFSS
                     FLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSGP
                     EMASLEAWLEEHEKYRSVA"
     gene            3042..3428
                     /gene="trbI"
                     /locus_tag="OHO85_004668"
     CDS             3042..3428
                     /gene="trbI"
                     /locus_tag="OHO85_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HCQ2673912.1"
                     /translation="MTITQKTTDVTAPRRSHWWWTVPGYLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            3425..4057
                     /gene="traW"
                     /locus_tag="OHO85_004669"
     CDS             3425..4057
                     /gene="traW"
                     /locus_tag="OHO85_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HCQ2673913.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVNEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            4054..>4803
                     /gene="traU"
                     /locus_tag="OHO85_004670"
     CDS             4054..>4803
                     /gene="traU"
                     /locus_tag="OHO85_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HCQ2673914.1"
                     /translation="MKRRLWLLMLFLFAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKL"
BASE COUNT         1115 a         1238 c         1389 g         1061 t
ORIGIN      
        1 cggtagcgtg tcagaacatg tcgggaggaa tattccgtga gtgatgaagc cgatgaagca
       61 tattcagtga cagaacaact gaccatgaca ggaataaacc ggatacgcca gaaaataaat
      121 gctcatggta ttcctgttta tctctgtgaa gcatgcggaa atcctattcc ggaatcccgg
      181 cggaaaatat ttcccggtgt gacgttgtgc gttgaatgtc aggcgtatca ggaaagacag
      241 agaaaacatt atgcataagt cagtcgcaga acatagtgat ttaattccgg atgaacatga
      301 gtggatattc agaaaacaga aatctttgta tatgcgccgg gaaatggcgc gttaattaca
      361 ggtattccct tcatggctac cgcatttctc gctttatttt tcaactaagg aattcatgtg
      421 aataacccac ttgaggccgt cactcaggcg gttaactccc tcgtcacagc actgaaactg
      481 cctgacgaat ctgcaaaggc caatgaagtt ctgggcgaaa tgagcttccc gcagttcagc
      541 cgtctgctgc cgtaccgtga ttacaaccag gaatccggtc tgttcatgaa tgacaccacg
      601 atgggcttta tgctggaagc catccccatc aatggggcga atgagtccat tgtggaggcc
      661 ctcgatcaca tgctgcgcac caaactgccg cgcggtattc cgttgtgtat ccatctgatg
      721 tccagtcagc tggttggtga caggattgaa tacgggctgc gtgagttctc ctggtcaggt
      781 gaacaggctg aacggtttaa cgccattacc cgggcctatt atatgaaagc ggcagcgaca
      841 cagtttccgc tgccggaggg gatgaatctg gccctgaccc tgcgccatta ccgggtgttt
      901 atctcgtact gttctccttc gaagaaaaaa agccgggccg acattctgga aatggaaaac
      961 ctggtgaaaa tcatccgggc gtcgttacag ggggccagta tcaccacaca gacggtggat
     1021 gcacaggcct ttatcgatat tgtcggggag atgattaacc ataacccgga ctccctgtac
     1081 ccgaaaagac gtcagctgga cccgtattct gatctgaatt atcagtgtgt ggaggacagt
     1141 tttgatctga aagtccgggc tgattacctg acgctgggcc tgcgtgagaa cggcaggaac
     1201 agcacggccc gcatcctgaa tttccatctg gcccgtaacc cggaaatcgc cttcctgtgg
     1261 aacatggccg acaactacag caacctgctg aacccggaac tgtccatctc ctgtccgttc
     1321 atcctgacgc tgactctggt ggtggaagac caggtgaaaa cccacagcga agccaacctg
     1381 aagtacatgg acctggagaa aaagtcgaag acctcctatg ccaaatggtt tccgtccgtg
     1441 gagaaagagg cgaaggagtg gggggaactg cgtcagcggc tgggctccgg tcagtcctcc
     1501 gtggtgtcct acttcctcaa catcacagca ttctgcaagg acaataatga aacggcgctg
     1561 aaagtggagc aggacatcct gaacagcttt cgtaaaaacg gttttgagct gatttcaccg
     1621 cgctttaacc acatgcgcaa tttcctgacc tgcctgccct ttatggccgg gaaagggctg
     1681 tttaaacagc tgaaagaggc cggagtggtt cagcgcgcag agagctttaa tgtggccaac
     1741 ctgatgccgt tagtggcgga taaccccctg acaccggcag gtctgctggc acccacctac
     1801 cgtaaccagc tggcgtttat cgatattttc ttccggggga tgaataacac caattacaac
     1861 atggcggtct gtggcacctc cggggccggt aaaaccgggc tgatacagcc acttatccgc
     1921 agcgtgctgg actccggggg ctttgccgtg gtgttcgaca tgggggatgg ctacaagtcc
     1981 ctgtgtgaga acatgggggg cgtatatctg gacggtgaaa ccctgcgctt taacccgttt
     2041 gcgaacatca ccgatatcga ccagtcagcg gagcgtgtcc gcgaccagct gtcggtgatg
     2101 gccagcccca acggtaacct ggatgaagtg catgaaggtc tgctgttgca ggcggtcaga
     2161 gcctcctggc tggccaaaaa gaatcaggcg cgcattgatg atgtggtgaa tttcctgaaa
     2221 aacgccagtg acagcgagca gtatgccggg tcaccgacca tccgcagccg tctggatgaa
     2281 atgattgtgc tgcttgacca gtacactgcc aacggcactt atggccggta ttttaactct
     2341 gatgagccgt ccctgcggga tgatgccaga atggtggtgc tggagctggg cggactggaa
     2401 gaccgtccgt cactgctggt tgcggtgatg ttctccctga ttatctacat cgagaacagg
     2461 atgtaccgca cgccgcgtaa cctcaagaaa ctgaacgtta ttgatgaagg ctggcgtctg
     2521 ctggacttca aaaatcacaa ggtcggtgaa tttattgaga aaggctaccg tacggcccgc
     2581 cgtcataccg gtgcctatat caccatcaca cagaacatcg tcgactttga ctctgacaag
     2641 gcctccagtg ctgcccgcgc ggcatggggt aactcctcct acaaaattat cctcagacag
     2701 agtgcgaagg agttcgcgaa atacaaccag ctgtatccgg accagttcca gccactgcag
     2761 cgcgacatga ttggtaagtt tggtgcggcc aaagaccagt ggttcagttc cttcctgctg
     2821 caggtggaaa accattcctc ctggcaccgc ctgtttgtgg acccgttaag ccgcgccatg
     2881 tacagctctg acggcccgga ttttgagttt gtgcagcaga aacgtaaaga ggggctgagt
     2941 attcatgagg cggtgtggca gctggcgtgg aagaagtcag ggccggagat ggcttcgctg
     3001 gaagcctggc tggaagaaca tgagaaatac aggagtgttg catgaccata acgcaaaaaa
     3061 cgactgacgt tacagccccc cgacgcagcc actggtggtg gacggtgccg ggatacctgg
     3121 caatggtgtt actgaacgca gccgtcagtt acggcattgt cagactgaac gcaccggtga
     3181 ctgccgcctt caacatgaaa cagaccgtgg atgcgttttt tgacagcgcc agtcagaaac
     3241 aactgtcaga agcacagtca aaagcccttt cagcgcgttt taacacggca ctggaagcca
     3301 gcctgcaggc gtggcagcag aaacaccacg cggtcattct ggtgtcgcct gctgtggtac
     3361 agggggcacc ggatatcacc cgggaaatcc agcaggatat tgcccggcga atgagggcgg
     3421 agccatgaga tgccgggggc tgattgccct gctgatatgg gggcagagtg tggccgccgc
     3481 cgatcttggt acctggggcg atctgtggcc ggtaaatgag ccggacatgc tgacggtgat
     3541 catgcagcgc ctgacggcac tggagcagtc cggtgagatg ggccggaaaa tggatgcctt
     3601 taaggagcgg gtgatccgca acagtctgcg gccccctgcc gtgcccggta tcggacgcac
     3661 ggagaaatac ggcagccggt tgtttgaccc gtccgtcaga ctggctgcgg atatccggga
     3721 taacgaaggg cgggtgtttg cccgtcaggg tgaagtgatg aatccgctgc agtatgttcc
     3781 ttttaaccag acgctgtatt tcatcaatgg cgatgatccg gcgcaggtgg cctggatgaa
     3841 acgccagacg ccgcccacac tggagagcaa aattatcctc gtgcagggca gtatcccgga
     3901 gatgcagaag tctctggaca gccgtgttta ctttgaccag aacggtgtgc tctgccagcg
     3961 cctcggcatt gatcaggtcc cggcacgggt gagcgccgtt cccggcgatc gcttcctgaa
     4021 ggtggaattt attccggcag aggagggcag aaaatgaagc gaaggctgtg gctgctgatg
     4081 ttattccttt tcgccggtca tgtccctgcg gcgtctgcgg attctgcctg tgaggggcgt
     4141 tttgtaaacc cgatcacaga tatctgctgg agctgtattt tcccgctctc gctgggcagt
     4201 atcaaagtca gtcagggtaa ggtccccgac acggcgaacc cgtcgatgcc cattcagatt
     4261 tgtccggcac cgccgccgct gttcaggcgt atcgggctgg ccattggtta ctgggagccg
     4321 atggcgttga cggacgtcac ccggtcaccg ggatgcatgg tgaacctggg cttcagcctg
     4381 ccggcttttg gtaaaacggc acagggaacg gcgaaaaagg atgagaagca ggtaaatggg
     4441 gcgttctatc acgttcactg gtacaaatac ccgctgacgt actggctgaa catcatcaca
     4501 tcgctgggct gtctggaagg tggtgacctg gatatcgctt atctttctga aatcgacccc
     4561 acctggacgg acagcagcct gaccaccatt ctcaatccgg aagctgtcat ctttgccaat
     4621 ccgatagcac agggagcctg cgcagcagat gcgattgcca gcgcctttaa tatgcctctc
     4681 gatgttctgt tctggtgtgc cggttcgcag ggaagtatgt acccgttcaa tggctgggtg
     4741 agtaatgagt ccagtccgtt gcagtcctcc ctgctggtca gtgaacgcat ggcgttcaag
     4801 ctg
//
LOCUS       DAJOWB010000221         4688 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJOWB010000221 DAJOWB010000000
VERSION     DAJOWB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.222"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..161
                     /locus_tag="OHO85_004671"
     CDS             <1..161
                     /locus_tag="OHO85_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673915.1"
                     /translation="ITLVILFCRRPHCRKVSVRQYYQKRVIFYPTTFQRTAFKKCLFL
                     MRDIIISS"
     gene            122..241
                     /locus_tag="OHO85_004672"
     CDS             122..241
                     /locus_tag="OHO85_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCQ2673916.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(720..986)
                     /gene="minE"
                     /locus_tag="OHO85_004673"
     CDS             complement(720..986)
                     /gene="minE"
                     /locus_tag="OHO85_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCQ2673917.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(990..1802)
                     /gene="minD"
                     /locus_tag="OHO85_004674"
     CDS             complement(990..1802)
                     /gene="minD"
                     /locus_tag="OHO85_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCQ2673918.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(1826..2521)
                     /gene="minC"
                     /locus_tag="OHO85_004675"
     CDS             complement(1826..2521)
                     /gene="minC"
                     /locus_tag="OHO85_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCQ2673919.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3041..3409
                     /locus_tag="OHO85_004676"
     CDS             3041..3409
                     /locus_tag="OHO85_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HCQ2673920.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3529..3930)
                     /locus_tag="OHO85_004677"
     CDS             complement(3529..3930)
                     /locus_tag="OHO85_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCQ2673921.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4172..4465
                     /locus_tag="OHO85_004678"
     CDS             4172..4465
                     /locus_tag="OHO85_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCQ2673922.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4537..>4688
                     /locus_tag="OHO85_004679"
     CDS             4537..>4688
                     /locus_tag="OHO85_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isomerase/hydrolase"
                     /protein_id="HCQ2673923.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETAL"
BASE COUNT         1208 a         1053 c         1096 g         1331 t
ORIGIN      
        1 cgattacatt agtcatttta ttttgccgac ggcctcattg tcgaaaagtc agcgtacgac
       61 agtattatca gaaaagagtg attttttatc caactacatt tcagcgcact gcgtttaaaa
      121 aatgcctctt tcttatgcgg gatatcatca tttcatcatg atgtctttgg tgagcggtga
      181 acacaataca cctgcgctgt ctcttcagga tgaatcccct ggtctggtgc ctgcgggctg
      241 atgttgcagc cgagctaagg tcacttagac gctactatca tttatcccat ggcatggagt
      301 caaaatcagt cgatacccgc agtatatatc gtgaactggt cgttctgacc atcaacgatt
      361 aaactgcgct tcggcacagt tttcgtttac aggatgttaa aagagaaaat tctggggcaa
      421 aaaaagcccg ccagttacgg cgggaaacct catcctatgg gagaacaatg aataatgaaa
      481 ttgcggggtt atcatctccc agtgtatcca cactaacaat aaggttattt actcagccag
      541 gcataaacat tttgttttgt gcgtgggaac agccttaagg tgtaaagggg gaggtggaaa
      601 tagcaatgag gagtatcagc aagaatactc gccgctttac cacaacgtgg atgagaggga
      661 tgaaaaactc aaggcagaga taactctgcc ttgaagataa atgcgctttt acagcgggct
      721 tatttcagct cttctgcttc cggtaaggtc acgttcagct caagaataga aatatcgcca
      781 tctttttgct caagctgtac ggttaccatc tcaggatcaa tttgtacgta tttacaaatg
      841 acctcaagaa tatctttacg caactgcggc agataatgcg gttctgcatc gctgcgacgg
      901 cgttcagcaa caataatctg cagccgttct tttgcaatgt tggctgtgtt tttcttccgc
      961 gagagaaaga aatcgagtaa tgccataact tatcctccga acaagcgttt gaggaagcct
     1021 ttcttctctt cttcaatgaa gcggaaagga cgttcttctc ccaacagacg ttctacggta
     1081 tctgcgtagg ctttacccgc atcggcgtta atgtcgagaa tgaccggttc accctggtta
     1141 gaggcgcgca atactgattg atcctctggg atcacgccga cgagtttgat gcgcaggatc
     1201 tccagcacat cttccatgct cagcatgtca cctctgctta cgcggcctgg gttatagcgc
     1261 gttaacagca ggtgctcttt aataggctct tcgccatttt ctgcgcggcg tgattttgac
     1321 gccagaatgc ctaaaatacg gtcagagtcg cgtactgagg agacttccgg gttggtggta
     1381 ataatggctt cgtctgcaaa atagagtgcc attaacgcac cggtttcaat ccctgccggg
     1441 gagtcacaaa cgataaattc aaaatccatc gctttcagat catcaagaac tttggcgacc
     1501 ccttcacggg tgagggcatc tttatcgcgt gtttgcgatg ccggcagaat atagagattt
     1561 tcagtacgct tatctttaat taacgcctga tttagcgttg catcgccctg aatgacgttg
     1621 acgaaatcgt aaacgacccg gcgttcacaa cccataatca ggtcgagatt acgcaggccg
     1681 atatcaaaat ctatcacgac agttttcttt cccttctggg ccaaaccagt ggcgatggcc
     1741 gcgctggagg ttgtcttacc aacaccccct ttgcccgaag taacaacaat aatgcgtgcc
     1801 atagaaattc cttgttaaaa agggatcaat ttaacggttg aacggtcaaa gcgttttcga
     1861 ctaactgcag tcgcgccgct ttgccataaa attctgctgg gatttgatca ctcagccagt
     1921 attcacctgc gatggacacc agttccgcca tcaggttcgt acaaaatatt tgcgtttccc
     1981 ggtcaccact tgcccctgcc agcgcacgac cgcgcatcat gccatagaca tgaatgttac
     2041 catcggcaat taattcggcc ccagcgctaa cgtggcttgt aacaatcaga tcacattgtg
     2101 gagcataaat acgctgaccg gaacgcaccg gggtatctat taaacgcgtt tttgtgaccg
     2161 gcgttgtatt ttgcgctgga gcctgcggtg cgggagctgg acgtggcgct ttttcctttc
     2221 cttccgtcag gataggcagc cccatttttt caatttcggc tttaagttgc gcatctttgc
     2281 agccacttac gccaataacc cgcaaaccgg ttgccgaaac cgccttatgc atcgctgacc
     2341 agtttaccgg gtcttccagt gcactgacgt tgagtacaac gggggcatgt tttaaaaatg
     2401 cgggggcctg agcgattttg tcttccagcg cctgatggat aaccttaggt tctgcctcat
     2461 gcagatgaac cacagataaa gtgaagctac tgcctttaag ctcgattggc gtgtttgaca
     2521 tcctggcctt actcaattag ctattaatca tcgccagcgc gcgatgatgt tccgaagact
     2581 ataaggcatg ttatagtctg gattatattg aggcaagtca ccctcccatt tattcagagt
     2641 aaaagtctat tctgtgataa atggcgctga ttcatagctt aaaaaatacc cttgtcaatc
     2701 aacccattgc cgtcgtactt ttgattcttc ttatttacgc ttcctttttc cgcaccctaa
     2761 tcgtgacagc atctccagac ctgtttaaat atccagtatt gaggcaagtg tattttttca
     2821 ccacgccagg ctttcttaac tttccatttt ttgcggcatc attaaagaac agatacccgt
     2881 tatcttaata tccgccacag aattgctcta tcatagaaac cgcagaagac gtctgatact
     2941 tctgtcaggt catcgtaact cgtccaggtc agcggttttg attacggatt gatgatgaaa
     3001 tgagcataag tgttgtacgg ttggattatc aggatgtgct atgaatatga tgcgtatttt
     3061 ttatatcgga ttgtcaggtg tggggatgat gttctcatca atggcgtctg gaaatgacgc
     3121 tggcggactt caatctccgg cgtgcggcgt tgtttgcgac ccgtatattt gtgtgaactc
     3181 agatggcatt tccccagagt taacaaggaa atatctcggc gaaaaagccg ctgaaaactt
     3241 acaatcatta caaggctacg atcccagcga atttacattc gctaacggtg tattttgcga
     3301 tgttaaagaa aaattatgtc gtgatgatcg ttattttggt gtagatggta agcggagtgg
     3361 aaaaatcaat caaaccacca caaagatgtt atttatgtgt cgtgagtgat gccttgttta
     3421 cattatgttc tattcggcat tgtctgctat tcgtcattaa cctcttgagc gacagagagg
     3481 agtggggcgt taacgtttat gacggggaga gtccccgtcg gttgacattt acttaatttg
     3541 aatatcgacg ttatattttt tagttttgtt tttacgggcg tcgtttcggg tttgcagcac
     3601 tcgcagctca tatttgccag aagcagggag tgagtattgt ccgtgactat cgagttctgg
     3661 ggaatatctg gataaatcaa cagaatcatc gattcctggc ccgaagaggt aagtatcagc
     3721 gccttcatta gagatactca catgcacttt ctgacctttt ttggcataga aggtatatgt
     3781 atcgtaatcg tatcccttta tttcgccgga gtactgagcg ctgctatggc cttttctgaa
     3841 ctcaacattg acattcttac ccgcagcaaa gctcgtggag gttagcagtg caagcagcaa
     3901 tacagcctta ctgatgctct tgattttcat tggtagtctc ctgttcagaa tgagtacgat
     3961 gaactgttat aatataacaa tcccttacgg gagaattcca gtggctttcc tgcgttcggt
     4021 aaaatcccgt cgcggcattt ttgccggggg gatgacttat aaagggggca tggactcaac
     4081 aaaccgtaga aatctacagc tggctggctt gtggcgcggg tttcattgat gttagcttat
     4141 gccaaaacct ggcatcttaa agagtaaaag tatgttttgt gtgatttatc gaagcagcaa
     4201 gcgtgaccag acctatttat atgtcgaaaa aaaagacgat ttttcgcgtg ttcctgagga
     4261 actgatgaaa ggttttggtc agcctcagtt agcgatgatt ctgccgctgg atgggcggaa
     4321 gaaactggtc aatgccgata ttgaaaaggt taaacaggca ttaaccgagc aaggttacta
     4381 tttgcagtta ccgccaccac ccgaagattt gctgaagcaa catctttccg tcatggggca
     4441 gaaaacagac gacactaaca aataaccgat atccggcggt ggcattatct ttgtcggcgc
     4501 gggttttcat atccacgata aggtgagggg aacgttatgt atcaacatca caactggcaa
     4561 ggtgcgctgc tggattatcc ggtgagtaaa gtagtctgtg ttggcagtaa ctatgccaaa
     4621 catattaaag agatgggcag cgcagtgccc gaagagccag tgctgtttat taaaccagaa
     4681 acggcact
//
LOCUS       DAJOWB010000222         4645 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJOWB010000222 DAJOWB010000000
VERSION     DAJOWB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.223"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..567)
                     /locus_tag="OHO85_004680"
     CDS             complement(<1..567)
                     /locus_tag="OHO85_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673924.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLL"
     gene            complement(662..868)
                     /locus_tag="OHO85_004681"
     CDS             complement(662..868)
                     /locus_tag="OHO85_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCQ2673925.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            2392..2961
                     /locus_tag="OHO85_004682"
     CDS             2392..2961
                     /locus_tag="OHO85_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCQ2673926.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEYTKAHCTGERNFGCSRS"
     gene            3221..3622
                     /locus_tag="OHO85_004683"
     CDS             3221..3622
                     /locus_tag="OHO85_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673927.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            3610..4044
                     /locus_tag="OHO85_004684"
     CDS             3610..4044
                     /locus_tag="OHO85_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCQ2673928.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            4378..>4645
                     /locus_tag="OHO85_004685"
     CDS             4378..>4645
                     /locus_tag="OHO85_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2673929.1"
                     /translation="MGTKVSDMQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISK
                     LALENGINANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQ"
BASE COUNT         1144 a         1156 c         1263 g         1082 t
ORIGIN      
        1 caggagcacg tactgatcca tcagatccgt cagtgctgcg ctctgcttct gacggttgat
       61 gatccattcc tccagctctt tttctgattt ccgtttcaga ttctccgtcc agtcgtcaag
      121 gcagtttccg gtcattaaat caagccagca aagccagacc agtttgaggc gcaactccgt
      181 gccaccaaac tgacggaaga ggctgatcag tttatttgcc agagaacgct gcctcagacg
      241 ttcacattca gacaggagaa tgccttccag catcggatga tgaaccagca gatgttcact
      301 ggtgcataaa ttcactatca gatcatcatc caggggcggt gtggcccgtt tgtcagggcc
      361 ataaatcagc cggtgcaggc tctggggtac gatggcaatc tccggttttt caccattgaa
      421 tgacacccaa ccgggcgtca gattatggaa gtagtagata ttcggatgac acacgcccca
      481 ggatacggcg tgccgggtga agccggtcag ccatttctgg tagctttcgt ggagccattc
      541 gtagcgggtc tgatactgtt gtttcatttt catattcctc acggggatat aacaggcagc
      601 agaatgccgc cggcaggccg gagattacgt ctgctgcggg tgaacggata acggtcagaa
      661 attacggacg ggactgtgca atacgcgcct gcagccaggc ttccacttca cttttcagcc
      721 agcgggagct gcgccccatt ttgatggggg caggaaagcc cccatctttg atgagtttgt
      781 aaaaccactt atcggttaag ccggtaagtt gagtgataaa cgccatgtcg accatctggt
      841 catccatcag cgaaacaggg gtagccatga gaatgttcct ccggatgttg ttaaaccagc
      901 cggtgtggtc atgaaaacag cgcgccacct tcactggcat aacaaccggg gagaaacgta
      961 aaaaataatc gggaatgtgg ctgtccggag ataaacgggg ggattgcgat ggcagagccg
     1021 tagcggaatc ccggggggtt gttgcgaaaa gatgcttccg cacgcacgcg cgataaagga
     1081 gggaggactt tcgtgagaca aggggaaaac tagcgttctg ataattgcag agttgttttc
     1141 ggaggtggtt acagtgagtc agcccgttcc tggcgaggag gagactgata tcaccgatgg
     1201 gataaggctg tctgattggt attataaata taagaagtca tcagagagta acgtaaagga
     1261 tttgccagag taagctaaga gagtaaatct ctccggtaac agagcgctgt gaagcattct
     1321 gtatcagagt gcaaatacat gaacgctgat gcgtaaaatc agcagtgaca cgccagctcc
     1381 ccctgtgccc attcctgtat aagacatgaa ctgagcgtat taatgctcct gctctgagac
     1441 tgagccggtg agtgaggggc tgagaaatcc ctcttgacac agctctaaac cagaaatact
     1501 tccccggttg ttgcggtgcg tccggatccc cgataacgga acactaaggc tgtattgcgg
     1561 gacaccgggt aagtggcagc ctgtaatgtg ttctgtcaac gccagaaaaa atgatttttc
     1621 acacgcgtta tagactcact cctgtggacc tggaagcccg taactctgcg gttgcccacc
     1681 gactgacgga actgaccgca aactttacga gtgcgtgcag ggcttcagtc tggtcacctg
     1741 agcgttcggt aacggaggcg cgggcggtaa taggctgatt ttcaggtatt accggcttac
     1801 agcgtgtggt tgatattgag gggctgtgag tttcgcgtat ttgtcagaaa cccagacgag
     1861 ttttcctgta tgggacgaaa taaggcagcg gcaatacctg gaaaaattta atatggtatg
     1921 aaggagaacc tgtcggttac gcttaatata cccggtacac tgtcagctca ggggatacat
     1981 gaaccgttat ctcaccagct acacagtata tacacactga cgctgacagt agtcagcagg
     2041 accacaataa tcagtgccag cagtatggta acgatacggt ctgtattccg gtaatagccg
     2101 aaggggtcga cagaaccaca atacgggcaa cacactgtat tatgagtaat cgcgtttcca
     2161 cagtccctgc acttcttttt gcgtatcacc atcactccct cctcacagcc ttacccgtaa
     2221 ccggtttttt acattaaaaa aacaacgctg aaaattcaac cgtcagtccg gagacgaccg
     2281 ttcgggttat cacagagtgc ctgagacagt gtcctgccgg aggtcacttc agggactctg
     2341 cgcatttttt acgacgtggc tattccgtat gaaccatacg gagatttaac catgacctac
     2401 aaatacaacc ccttctggca gcaacgtatt cgtgagacgg tgcggcacgc actgaatgtt
     2461 catccccgcc tgacggcatt gcgggttgac ctgcgtttcc cggatgtacc ggcagcaacg
     2521 gacgcagctg tgatatcccg cttcatcaat gccctgaaag cccgaatcga cgcttaccag
     2581 aaacgtaagc atcgggaagg taaacgcgtg catcccacaa ccctgcatta cgtctgggcc
     2641 cgggagtttg gggagtgcaa aggtaaaaaa cactatcacc tgatgctgct ggtcaaccgg
     2701 gatacctggt gtcgtgccgg tgattaccgc gctccggagt ctctggccgg gatgattaaa
     2761 caggcgtggt gcagtgccct gggagtggat gtcgggtgcc atgccacgct ggtgcatttt
     2821 ccggcctggc cggcggtgtg gctggcgcgt aatgatgaca ccggctttca gcaagtgctg
     2881 gaacgtgctg actatctggc gaaggagtat accaaagctc actgcaccgg tgagcgcaat
     2941 tttggctgta gccggagttg agcacgactg gcgcgccaat atccggcaaa ccaaaagtat
     3001 gggttgttgt ctggcggcaa cgatgtatcc actcgcccac tgactgtttt ttacgtcagt
     3061 gttccattgt gtgacaacca cagtgctgta acagcaacca gcctgcttta cccaaactcc
     3121 agtccagatt ttaactgaac cgccattcac accctgacca cgctgcccgt accgggacgg
     3181 ttgttgcctg cgtgtcgtct ggcggctaag gaatatgact atgtacgcaa aatcctttat
     3241 cgctcttgat ggcaacggac gtctgacggg cgcccgtact gcacaggccg caccttatgc
     3301 tcactacacc tgccacttgt gtggcagtgc actcagatac catccgcaat acgacactga
     3361 acttccctgg tttgaacaca ctgacgacag gctgacagag cacggtcaac agtgccctta
     3421 tgtcaggccg gagcgcagag aaatacagtt gattaaacgt ctgcagcaat tcgtaccgga
     3481 tgccttaccc gtggtgcgta aagccagctg gcactgcaga caatgtcacc acgattatta
     3541 tggggagcag tactgcacac actgccagac cggaggattc agtattcccc ggacaactca
     3601 ggaggaaata tgcgaattct gaactgttat atggcgaatg acagcaaagg ccattttgtt
     3661 acggcgaaag aagctgcgaa gcacaaccga caggacgttt tgtgctgtgt gtcctgtgga
     3721 tgcccgttaa cacttcagcg gggcaatgac ggacaaccac cgtggtttga acatgaccag
     3781 atgactgtcg ctgaaaaaat cctgctgcga tgtacctggc ttgacccggc agagaaagag
     3841 gcccgtcgtt tgcatctgca gggcatgacg gttccggatt atacggtgaa ggtgagaaag
     3901 tggttttgtg tgatgtgtga cgaagattat gagggggaaa agtgctgccc acgctgcggt
     3961 accggggtat acagcagggc gtgggggcgg caggaggtgc cgtcggaaga tgccagggct
     4021 gataatccgt tacagaggct gtaatggttg cctccggagc agtttgcggt gatgcctttc
     4081 ccgggaatgg attgccagtg cggggactgt ggagcttaat tccggtgttg gtggcctttc
     4141 cattgatttt atgccaacag ccccctgtga ctgacaggct gcctcgtcat tccattcgta
     4201 tctccagtaa cagggggttg tattgtattt tattgtaccg gttcaggtgt gcgtttcccg
     4261 gcgcgtctgc accggcttaa ccaaattcaa cagggataaa atagtggtaa gcgtacagcc
     4321 tgaaccgtct ggtcagaatc tgacgaatta gacaaagtgg tgtccaccaa ataagtagtg
     4381 ggaaccaaag tgtcagatat gcagaaaaat gtgactcccg gcaggcgaaa aggctgccct
     4441 aattatcctc ccgaatttaa acagctgctc gttgctgcct cctgtgaacc cgggatatcc
     4501 atctcaaaac ttgctcttga aaatggcatt aacgccaatc tgttgttcaa atggcgacaa
     4561 caatggcgcg agggaaagct gctattacct tcttcagaga gcccccagct acttcctgtg
     4621 actctcgatg cagctgccga acagc
//
LOCUS       DAJOWB010000223         4644 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJOWB010000223 DAJOWB010000000
VERSION     DAJOWB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.224"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..442)
                     /locus_tag="OHO85_004686"
     CDS             complement(<1..442)
                     /locus_tag="OHO85_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCQ2673930.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVA"
     gene            671..1279
                     /locus_tag="OHO85_004687"
     CDS             671..1279
                     /locus_tag="OHO85_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCQ2673931.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1377..2768
                     /gene="selA"
                     /locus_tag="OHO85_004688"
     CDS             1377..2768
                     /gene="selA"
                     /locus_tag="OHO85_004688"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCQ2673932.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQTPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2765..4609
                     /gene="selB"
                     /locus_tag="OHO85_004689"
     CDS             2765..4609
                     /gene="selB"
                     /locus_tag="OHO85_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCQ2673933.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVVLTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT         1044 a         1194 c         1362 g         1044 t
ORIGIN      
        1 tcgccacgcc gccgcgcacc agccacagtg actcgctgcg gctgtaaccg tcctcaccgg
       61 gaaacaggtg ctcaaaatac aggcttctgc cgccgttatc actgagtatc agtgtgttat
      121 cgcgcagctg taagcggata tccgcaggca ttttccagcc ggggccgagg ctccccaccg
      181 gcgcgggcgt ttttgtccgg taactgctgt aggtgcggga gaggatgaac ggcagcgggc
      241 cgggcagggc gatgtcggtt tcaccgggaa ggacctttgc accgagcagg ggattgaccg
      301 gatggccgga cgtcactccg ccggggcaca ccgaacaggc cacgccggtg ggggcaccaa
      361 tgcgcacccc ggctgaaccc tgaacaatgc taccgccata ctgcgtcatg tcgccctgac
      421 gcgctgccgg ttttccgctc atctgctcat ccttgtttta tcgggttatt tgtctgcttc
      481 caggcaaaac cctacagatt tataagaaag ccttaagtga gatatccatc agatagaaac
      541 aattaggaaa atatttatat caccatgata aatatgtgat ttatttggaa gtaacaaacc
      601 atcaacattt tctgcccttt ttcccatcac caggccatac tgatattttt cctcgcacaa
      661 aggactcggc atgaaactcg tcggtagcta caccagcccg tttgtacgca aactttctat
      721 tctgttgtta gaaaagggca taactttcga atttattaat gaactgccct ataacgcgga
      781 caacggcgtg gcgcaattta acccgttagg aaaagtgccg gtgctggtga ccgaagaggg
      841 cgaatgttgg tttgattcgc cgatcatcgc tgaatatatt gaattaatga atgtcgctcc
      901 ggcgatgttg ccgcgcgatc cgctggagtc gttgcgggtg cgcaaaattg aggcactggc
      961 ggatggcatt atggatgccg ggctggtatc ggtgcgtgaa caggcgcgtc cagcggcgca
     1021 gcagtctgaa gatgaattgt tacgccagcg ggagaaaatc aaccgcagtc tggatgtgct
     1081 ggaaggatat ctggtcgatg gcacactcaa aaccgatacg gtcaatctgg cgactatcgc
     1141 cattgcctgt gccgtcggat atctcaattt ccgccgcgtt gcgccaggtt ggtgtgtcga
     1201 ccgcccacac ttagtcaaac tggtcgaaaa cctgtttagc cgcgaaagtt ttgctcgcac
     1261 cgaaccgcca aaggcttgat gcgcgatatg tcctcctgac ccatctcacg ttacaatccg
     1321 tggttatgtt aaacgccctt ctccgtgtga gagggccttg atcagccagg tttcctatga
     1381 caaccgaaac gcgttccctc tatagtcaac ttccggctat tgatcgctta ttgcgcgata
     1441 gctccttcct ttctttgcgt gatacttatg gtcacacccg cgtggtggaa ttgttgcgtc
     1501 agatgctcga cgaagcgcga gaagtgattc gtggcagcca gacgctgcct gcgtggtgtg
     1561 aaaactgggc gcaagaagtc gatgcccggt tgacgaaaga agcgcagagc gcgctgcgtc
     1621 cggtgatcaa cctgacggga accgtgctgc ataccaacct tgggcgagct ttacaggcgg
     1681 aagccgcggt ggaagccgtt gcgcaggcta tgcgttcgcc agtgaccctc gagtacgatc
     1741 tggacgacgc cggacgcgga catcgcgatc gggcgctggc gcaactgctg tgccgtatta
     1801 cgggggcgga agatgcctgt atcgtcaata acaatgcggc ggcggtgtta ttgatgttgg
     1861 cggccactgc cagcggaaaa gaggtggtgg tatctcgcgg cgaactggtg gagattggcg
     1921 gcgcgtttcg tattcccgat gttatgcgtc aggcaggctg caccctacac gaagtaggga
     1981 ccaccaaccg cacgcacgcg aatgattatc gtcaggcggt gaatgaaaat accgcactgt
     2041 tgatgaaagt acataccagt aactacagca ttcaggggtt caccaaagcg atagatgaag
     2101 cggaactggt ggcgctcggc aaagagctgg atgttcccgt agtgactgat ttaggcagtg
     2161 gctcgctggt cgatcttagc cagtacggtt tgccgaaaga gccaatgccg caggagttga
     2221 ttgcggcggg cgtcagtctg gtgagtttct ccggcgacaa gttgttaggc gggccgcagg
     2281 caggaattat tgttggtaaa aaagagatga tcgcccgcct gcaaagccac ccgctgaagc
     2341 gtgcattacg cgcggataaa atgaccctcg cggcgctgga agccacgttg cgtctttatt
     2401 tacaccctga agctctgagt gaaaaattac cgaccctgcg cctgcttacc cgcagcgcag
     2461 aggtcattca aatccaggca caacgtttac agacccccct tgccgcacat tacggcgcgg
     2521 agtttgcggt acaggttatg ccatgtcttt cgcagattgg cagtggttcg ctgccggttg
     2581 atcgcctgcc gagcgcggca ttaacgttta caccccatga tggacgcggt agccaccttg
     2641 agtcattagc cgcccgctgg cgtgaattgc cagtgccggt gattggtcgt atttatgacg
     2701 gacgattgtg gctggattta cgctgccttg aagatgagca acggtttttg gagatgttgt
     2761 tgaaatgatt attgcgactg ccggacacgt tgaccacggc aaaacaacct tattgcaagc
     2821 gattactggc gtaaatgctg accgtctgcc ggaagaaaaa aagcgcggca tgaccatcga
     2881 tctcggctat gcctactggc cgcagccgga tggtcgcgtg cctggtttta tcgacgttcc
     2941 cggtcatgaa aaatttcttt ccaacatgct ggcgggcgtt ggtggtatcg atcacgcgct
     3001 gttggtggtg gcgtgcgatg acggtgtgat ggcacagacc cgtgagcatc tggcgatttt
     3061 gcagctgacc ggtaacccga tgctgacagt ggtgctgacc aaagccgatc gcgtggacga
     3121 agcgcgtgtt gatgaggttg aacgtcaggt aaaggaggtg ctgcgggaat acggttttgc
     3181 tgaggcaaaa ctgtttatca ccgcagcaac cgaaggtcga ggaattgatg ccctgcgcga
     3241 gcatctgctt cagttaccag aacgtgaaca cgccagccaa catagtttcc gcctcgcgat
     3301 cgaccgcgca tttaccgtaa aaggtgccgg gctggtcgtc accggtacgg cgttaagcgg
     3361 ggaagtgaag gtaggcgatt cactatggct aactggtgta aataaaccga tgcgtgtacg
     3421 tgcgctgcat gcacaaaacc agccaacaga aaccgcccat gccgggcagc gtatcgcgct
     3481 taacatcgcg ggtgatgcgg aaaaagagca gattaaccgt ggcgactggc tgcttgccga
     3541 tgcgccgcca gagccgttca cacgggtgat tgtcgagctt caaacccata cgccgctgac
     3601 ccagtggcag ccgctgcata ttcaccacgc cgccagccac gttacgggac gcgtttcgct
     3661 gctggaagat aaccttgccg agctggtgtt cgacacgccg ctgtggctgg cggataacga
     3721 tcgcttagtg ctgcgcgata tctcggcccg caacacgctg gcgggggcgc gtgtggtgat
     3781 gcttaacccg ccgcgtcgcg gtaaacgtaa gccggaatat ctgcaatggc tggcgtctct
     3841 tgcacgggcg cagagtgatg ccgatgcgtt atctgttcat ctggaacgcg gcgcggttaa
     3901 ccttgcggat ttcgcctggg cgcgccagct caacggcgaa gggatgcgcg aattgctgca
     3961 acagcctggc tatattcagg ctggttatag cttgttgaat gcgccggttg ccgcccgctg
     4021 gcagcggaaa attctcgaca cattagcgac ttatcatgag caacatcgcg atgaacctgg
     4081 gcctgggcgc gaacgtctgc gacgtatggc gttgccaatg gaagatgaag cgctagtact
     4141 gttgctgatt gaaaagatgc gcgaaagcgg cgacatcctc agtcatcacg gttggctgca
     4201 tctgccggat cacaaagcgg gcttcagcga agagcagcag gccatctggc aaaaagcaga
     4261 gccgctgttt ggtgacgagc cgtggtgggt gcgtgatctg gcaaaagaga cgggtaccga
     4321 cgagcaggca atgcgcctga cgctacgcca ggcggcgcag caaggaataa ttaccgcgat
     4381 cgtcaaagat cgttattacc gtaacgatcg gattgtcgaa tttgccaata tgatccgcga
     4441 tctcgatcag gagtgtggtt caacctgcgc ggcggatttc cgcgatcgct taggcgtagg
     4501 ccgaaagctg gcaattcaga ttctggaata ttttgaccgc attggcttta cgcgtcgtcg
     4561 tggaaatgat catttattgc gcgacgcatt attatttccg gaaaaataag gaaatgatta
     4621 attaattttt aaaataaatt aata
//
LOCUS       DAJOWB010000224         4642 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJOWB010000224 DAJOWB010000000
VERSION     DAJOWB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.225"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(213..2546)
                     /locus_tag="OHO85_004690"
     CDS             complement(213..2546)
                     /locus_tag="OHO85_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCQ2673934.1"
                     /translation="MSGMKVSQAVKAARGHWARILPALGMNILKNRHQPCPVCAGKDR
                     FRFDDLEGRGTWFCNQCGAGDGLALVSKVLNVDISEAADRIHGLTHSLSSASSGVWTS
                     TADTDGGKQAAAALAARLLQASRESAGNTYLTHKGFPEHICHELTSGHKTGGVMFHPG
                     DLIVPLYNADGELVNIQLISGNGSKCFLKGGQVKEAYHLIEGGGSSVRRMWIAEGYAT
                     ALTIRHLTGEAVMVAFSSVNFLSLTSVAHNKYPGYQLIIAADRDLNGSGQNRAETAAK
                     ACQCDIVLPPVFGDWNDALTQYGEESTRKAILEALKPHNASPFDTMSEAEFTAMSVSE
                     KAQRVREHYRDALAVDPNGQLLSRYESGAWKVISQSDFARDVAALFQRLGAPFSSGKI
                     ASLVETLKLIVPQQQNPARHLIGFRNGVLDTRTGLFSPHCKENWLRTLCEVDFTPPVE
                     GETLETHAPAFWRWLDRAAGHKPEKRDIILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     ILAEIATMLAGEDNATSATIETLESPRERAALIGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYQNAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVILHFPEQIAPEERD
                     PQLKDKIARELAVIVRQLIQRFSDPMSARTLLQSQQNSDEALTIKRDADPAFDFCGYL
                     EALPDTNGMFMGNANIVPRQPRTYLYHAYLVYMEANGYKNTLSLTMFGKGLPVMLKEY
                     GLHYEKRRTNQGMQTNLTLKEESNADWLPKCDEPTIK"
     gene            complement(2561..2884)
                     /locus_tag="OHO85_004691"
     CDS             complement(2561..2884)
                     /locus_tag="OHO85_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 family protein"
                     /protein_id="HCQ2673935.1"
                     /translation="MKSRTLPLSLRLALYRRAVACAWLSICHQHNRHLQLTLDEIETA
                     IARELEGFYLRQHGQTKGMEIACALLSDLMESGPLMSCPALSQLGIAVMDELCVRHII
                     KPVLH"
     gene            complement(2881..3105)
                     /locus_tag="OHO85_004692"
     CDS             complement(2881..3105)
                     /locus_tag="OHO85_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014882693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673936.1"
                     /translation="MNHAAISYDDIVRLKHLRNVGEFVTGMAVLQDCYEKPAGAQCEQ
                     LVSLIYLMTEQLDGVVQRCQDDLMNKEVVQ"
     gene            complement(3105..3659)
                     /locus_tag="OHO85_004693"
     CDS             complement(3105..3659)
                     /locus_tag="OHO85_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCQ2673937.1"
                     /translation="MMMPERINPHFSGLHPAVISRYSFSAAAKSAAGIGVPRNSMATP
                     DAPSVFFFVEALAHPFSRLWCLCVHRGSFEIMAVRAGQPSGWPVSIEAGYANPVRAAT
                     SEIGVSGGSNNRYSMEAAIMATTLTPFYPQYVFVLAAIRRADTQPRICMLRTVACDER
                     FARLSLVRDYVLSLSARLPAGEVM"
     gene            complement(3656..3916)
                     /locus_tag="OHO85_004694"
     CDS             complement(3656..3916)
                     /locus_tag="OHO85_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCQ2673938.1"
                     /translation="MHDISVSAPAPAAPLFPVLNQHERFLRLPEVMHLCGLSRSTIYD
                     LISRDAFPKQIPLGGKNVAWAQSEVSAWMADRISARGRGCDA"
BASE COUNT         1253 a         1187 c         1067 g         1135 t
ORIGIN      
        1 atacttattg aactttaaaa aaatcacgct aaataggtct tctatattca aaaaaactct
       61 tttttcaata aattgttcac actgttcact ttggattttt accattcaaa atcaaggaaa
      121 tagaagttaa ctgtacggtg aagagtgaac agataactct acattcttgt gccagcaaaa
      181 ataaaacccg gcatcagccg ggttagttgt aattatttga ttgttggctc atcgcacttg
      241 ggtagccagt ctgcattact ttcctctttt aaagtgaggt tagtctgcat tccctgatta
      301 gttcgccgtt tctcataatg tagcccgtac tccttcagca tgactggcaa ccctttgcca
      361 aacatcgtaa gactcagggt gtttttatat ccattagcct ccatgtatac gaggtaggca
      421 tggtaaaggt aggtacgtgg ctggcgtggg acaatattgg cgttccccat aaacatgccg
      481 ttggtgtcag gtaatgcctc aagatagcca caaaaatcaa atgctggatc agcatcacgc
      541 ttgatggtga gagcctcatc agaattctgc tgcgactgaa gcaatgttct ggcgctcatc
      601 ggatcgctga aacgctgtat gagctggcga acaatcacag ccagttctcg tgcaatttta
      661 tctttcagct gcggatcgcg ttcttccggt gctatctgct caggaaaatg cagaataact
      721 ctcctgcggg acacaccacc actgcgatca gtgaagcgca taggattatt atttacggcc
      781 aggataaccg ccggaatatg tgttgaataa gcattctgat atttggggtc caccgatacc
      841 gcatcgccac cagttatagc cttaagcccg gcgccgtcgc cgctccactt ctcctgatca
      901 ggcaggcgaa taagagaaaa acctatcaga gccgcacgtt ctcgcgggga ctccagcgtt
      961 tcaatggtcg cagaggtggc gttatcctct ccagcaagca tggtcgcaat ttcagccaga
     1021 atacttttgc cacttccacc agggccagtc acttccagaa agagctgcca gtcatagcga
     1081 ttcgccagca ccataaataa cgccgcaaga ataatgtcgc gtttttctgg cttgtgtccg
     1141 gcagcccggt ccagccagcg ccagaatgcc ggggcatgag tttcaagcgt ttccccctct
     1201 accggtggcg tgaaatcgac ttcacacagg gtacgcagcc agttctcttt acagtgcgga
     1261 ctaaacagcc ctgttcgcgt atcgaggacg ccattacgaa aaccgatcag atgacgtgcc
     1321 ggattttgct gctgcggaac aattaacttt aatgtttcca ccagtgaagc aatttttcct
     1381 gaggaaaacg gtgcaccgag gcgctggaaa agggctgcga catctcgggc aaaatctgac
     1441 tgagaaatca ctttccacgc tccagactcg tagcgggata aaagctgccc gttcggatca
     1501 accgccagtg catccctgta atgctcccga accctctgag ctttttcgct gacactcatc
     1561 gctgtgaatt ctgcttcact cattgtgtcg aaaggactgg cgttatgtgg cttcaaagcc
     1621 tcaagaattg ccttccgggt ggattcctct ccgtattggg taagcgcatc attccagtca
     1681 ccaaaaaccg gtggcagcac aatgtcacac tgacacgctt ttgcagcagt ttcagccctg
     1741 ttctggcctg aaccattcag atcgcggtcc gctgcaataa ttaactgata tcccggatac
     1801 ttgttatggg caacgctggt cagagaaaga aagttgaccg acgaaaatgc caccatgacg
     1861 gcttctcctg tcagatgacg aatggtaagc gccgtggcgt aaccttccgc aatccacatc
     1921 cttcttacag aactcccgcc cccttctatc agatgatagg cctccttaac ctgacctcct
     1981 ttaagaaaac acttgctacc attgccactg ataagctgaa tattcaccag ttccccgtca
     2041 gcgttataga gcgggacgat caaatcaccg gggtggaaca tcaccccacc ggttttatga
     2101 cctgaagtca gctcatggca aatatgctcc gggaaacctt tatgtgtaag ataagtattt
     2161 ccggcagatt cacgcgaggc ttgcagtagt cgcgcggcaa gtgctgctgc cgcctgtttc
     2221 ccgccatctg tatctgcggt tgacgtccag actccagaag aggctgaaga cagactatgt
     2281 gtcagtccat gtatcctgtc tgctgcttca ctgatatcca cattcagtac cttgctgaca
     2341 agcgctaaac cgtccccagc cccgcactgg ttgcagaacc atgttccacg tccttccaga
     2401 tcgtcgaagc gaaaacgatc tttaccggcg cagaccggac aaggctgatg gcgatttttc
     2461 agtatgttca tacccagcgc tggcaaaatc cgcgcccagt gaccgcgggc agccttcacg
     2521 gcctgactga ctttcattcc tgacatgatg cagttctccc tcagtgtaaa accggcttta
     2581 taatgtgacg gacgcataac tcatccatta cggctattcc gagctgggag agtgcggggc
     2641 aggacattag aggaccggat tccatcaggt ctgaaagcag ggcgcaggcg atttccatgc
     2701 cttttgtttg cccgtgctgt cgcagataaa aaccttcaag ctcacgggca atggcggttt
     2761 caatttcatc cagagtgagc tgcaggtggc ggttgtgctg atggcagata ctcagccagg
     2821 cgcaggcaac cgcgcggcga tataacgcca gccgaagtga gaggggaaga gtgcgtgatt
     2881 tcattgcacc acctccttgt tcatcaggtc atcctggcaa cgctgtacca cgccatcaag
     2941 ctgttctgtc atcagataaa tcagggaaac cagttgctca cattgagcac ctgcaggttt
     3001 ttcatagcag tcctgaagaa cagccattcc ggtgacaaat tcccccacgt tacgaagatg
     3061 cttgaggcgg acaatatcgt cataagaaat tgcggcgtga ttcatcacat cacctccccg
     3121 gcaggcagcc gtgcagaaag ggagagcaca taatcacgga ccagcgaaag gcgtgcaaag
     3181 cgctcatcgc aggccacggt gcgaagcata cagatgcggg gttgagtgtc agcacggcgg
     3241 attgcggcaa gcacaaagac atattgcggg taaaacgggg tgagggttgt agccatgatg
     3301 gcagcctcca ttgagtagcg gttattgcta ccaccggaaa cgccaatttc actggtggca
     3361 gcccgaacgg ggttggcgta accggcctca atggataccg gccagcccga aggctgcccc
     3421 gcccggactg ccattatctc gaaagaacca cggtgtacgc ataaacacca cagcctggaa
     3481 aatgggtgtg ccagagcttc gacgaaaaaa aagacgcttg gcgcgtctgg tgtagccatt
     3541 gagtttcgcg gaacgccaat tccggctgcc gattttgcgg cagcggaaaa actatacctg
     3601 gaaatgactg caggatgcaa gccagaaaaa tggggattaa tgcgttcagg catcattatg
     3661 catcacatcc ccgtccgcgg gcacttatac gatccgccat ccatgcgctt acctctgact
     3721 gtgcccaggc aacattttta ccaccaagcg ggatctgctt cgggaaagca tcgcggctta
     3781 tcaggtcgta tatagttgag cgggacaagc cgcataaatg catcacctcg ggaagacgta
     3841 aaaatcgctc atgctggttc agcacaggaa ataatggcgc agccggagct ggcgcagaaa
     3901 cagaaatatc atgcatttat ctaccctctt taattccttc atcagtccgg ataaatcccc
     3961 ccggattcag gtagttcctt attatgtata tatttctcgc ttacgcaccc attatttatt
     4021 tagcgtcaca tattgattta atggaattta aaactatatc aaaccacata aaatggcatg
     4081 atagatatta tgtctgcgaa ctataaatac agggacatta ataggaacta actttcatcc
     4141 aggggttact accccataag tcatagaact taatggtact gaaaccgcaa cccattcagc
     4201 agggatgcac aatttctaca tatggcttta cattgcaaac ctaacttcag gatatactca
     4261 tgaattgcat caagctcaga agaattagaa ccgcataaaa tcaataacca cgcattctta
     4321 acctaaaaat atatccaaat aaattcataa aagaccgagc agttctagtt ttataaaaat
     4381 cagattgaaa tgcaacacca gggaaagggg ggaatcatgt tatttcccgg caaagaaaaa
     4441 tcgtctattt catcatctgt tgaatactac tgaagagtgg tgaatagttg tgaggaacaa
     4501 taatgaaata atcattcgga ataaaaattt cacttttgat gaaaaaaaat cataaatcac
     4561 gctaccttcc gatgaacact catgaacagt cactgaatac tgccttgtca acccttcacc
     4621 tcttaactag ctgtattatc aa
//
LOCUS       DAJOWB010000225         4610 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJOWB010000225 DAJOWB010000000
VERSION     DAJOWB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.226"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..995
                     /locus_tag="OHO85_004695"
     CDS             <1..995
                     /locus_tag="OHO85_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2673939.1"
                     /translation="SVGLCLVHVACDKRPWLEGLNVEMDWQMSGKPLLLYLDNAAEFK
                     SEALRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDY
                     DSENKAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAVARVGVPAVVTRATS
                     FLVDFLPILRRTLTRTGFVIDHIHYYADALKPWIARRERWPSFLIRRDPRDISRIWVL
                     EPEGQHYLEIPYRTLSHPAVTLWEQRQALAKLRQQGREQVDESALFRMIGQMREIVTS
                     AQKATRKARRDADRRQHLKTSARPDKPVPPDTDIADPQADNLPPAKPFDQIEEW"
     gene            998..1858
                     /locus_tag="OHO85_004696"
     CDS             998..1858
                     /locus_tag="OHO85_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HCQ2673940.1"
                     /translation="MDEYPIIDLSHLLPAAQGLARLPADERIQRLRADRWIGYPRAVE
                     ALNRLEALYAWPNKQRMPNLLLVGPTNNGKSMIVEKFRRTHPASSDADQEHIPVLVVQ
                     MPSEPSVIRFYVALLAAMGAPLRPRPRLPEMEQLALALLRKVGVRMLVIDELHNVLAG
                     NSVNRREFLNLLRFLGNELRIPLVGVGTRDAYLAIRSDDQLENRFEPMMLPVWEANDD
                     CCSLLASFAASLPLRRPSPIATLDMARYLLTRSEGTIGELAHLLMAAAIVAVESGEEA
                     INHRTLSMAC"
     gene            1960..3483
                     /gene="istA"
                     /locus_tag="OHO85_004697"
     CDS             1960..3483
                     /gene="istA"
                     /locus_tag="OHO85_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS1326 family transposase"
                     /protein_id="HCQ2673941.1"
                     /translation="MINVAILSAIRRWHFRDGASIREIARRSGLSRNTVRKYLQSKVV
                     EPQYPARDSVGKLSPFEPKLRQWLSTEHKKTKKLRRNLRSMYRDLVALGFTGSYDRVC
                     AFARQWKDSEQFKAQTSGKGCFIPLRFACGEAFQFDWSEDFARIAGKQVKLQIAQFKL
                     AHSRAFVLRAYYQQKHEMLFDAHWHAFQIFGGIPKRGIYDNMKTAVDSVGRGKERRVN
                     QRFTAMVSHYLFDAQFCNPASGWEKGQIEKNVQDSRQRLWQGAPDFQSLADLNVWLEH
                     RCKALWSELRHPELDQTVQEAFADEQGELMALPNAFDAFVEQTKRVTSTCLVHHEGNR
                     YSVPASYANRAISLRIYADKLVMAAEGQHIAEHPRLFGSGHARRGHTQYDWHHYLSVL
                     QKKPGALRNGAPFAELPPAFKKLQSILLQRPGGDRDMVEILALVLHHDEGAVLSAVEL
                     ALECGKPSKEHVLNLLGRLTEEPPPKPIPIPKGLRLTLEPQANVNRYDSLRRAHDAA"
     gene            3473..4255
                     /gene="istB"
                     /locus_tag="OHO85_004698"
     CDS             3473..4255
                     /gene="istB"
                     /locus_tag="OHO85_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS1326 family helper ATPase
                     IstB"
                     /protein_id="HCQ2673942.1"
                     /translation="MQHEGHVRILKSLKLFGMAHAIEELGNQNSPAFNQALPMLDSLI
                     KAEVAEREVRSVNYQLRVAKFPVYRDLVGFDFSQSLVNEATVKQLHRCDFMEQAQNVV
                     LIGGPGTGKTHLATAIGTQAVMHLNRRVRFFSTVDLVNALEQEKSSGRQGQIANRLLY
                     ADLVILDELGYLPFSQTGGALLFHLLSKLYEKTSVILTTNLSFSEWSRVFGDEKMTTA
                     LLDRLTHHCHILETGNESYRFKHSSTQNKQEEKQTRKLKIET"
     gene            complement(4431..>4610)
                     /locus_tag="OHO85_004699"
     CDS             complement(4431..>4610)
                     /locus_tag="OHO85_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCQ2673943.1"
                     /translation="SALIEACRHAARTQGCAKLRLDCHPNLRGLYERLGFTHVDTFNP
                     GWDPTFIAERLELEI"
BASE COUNT         1020 a         1331 c         1339 g          920 t
ORIGIN      
        1 tttcggttgg cctgtgcctc gtgcatgtcg cctgcgacaa gcgcccttgg ctggaaggac
       61 tgaacgtgga aatggattgg cagatgagcg gcaagccctt gctgctctac ctagacaacg
      121 cggccgagtt caagagcgag gccctgcgcc ggggttgcga gcagcatggc atccggctgg
      181 actatcgccc gctgggacag ccgcactatg gcggcatcgt ggaacggatc atcggcacgg
      241 cgatgcagat gattcacgac gaactgccgg gaacgacctt ctccaaccct gaccagcgcg
      301 gcgactacga ttccgaaaac aaggccgccc tgacgctgcg cgagctagag cgctggctca
      361 cattggcggt cggcacctac cacggttcgg tgcacaacgg cctgctccaa ccgccggccg
      421 cgcgctgggc cgaggccgtg gcgcgtgtcg gcgtaccggc cgtcgtcaca cgcgctactt
      481 cgttcctggt cgattttctg ccgatcctcc ggcgcacgct gacccgcacc ggctttgtca
      541 tcgaccacat ccactactac gccgatgcgc tcaagccgtg gattgcgcgg cgtgaacgct
      601 ggccgtcctt tctgatccgg cgcgatccgc gcgacatcag ccgtatctgg gtcctggaac
      661 cggagggaca gcattacctg gaaattccct accgtacctt gtcgcatccg gctgtcaccc
      721 tctgggaaca acggcaggcg ctggcgaaac tgcggcagca agggcgcgaa caggtggatg
      781 agtcggcgct gttccgcatg atcggccaga tgcgtgagat tgtgaccagc gcgcagaagg
      841 ccacacgcaa ggcgcggcgt gacgcggatc gccgccagca cctcaagaca tcagctcggc
      901 cggacaagcc cgttccgccg gatacggata ttgccgaccc gcaggcagac aacttgccac
      961 ccgccaaacc gttcgaccag attgaggagt ggtagccgtg gacgaatatc ccatcatcga
     1021 cctgtcccac ctgctgccgg cggcccaggg cttggcccgt cttccggcgg acgagcgcat
     1081 ccagcgcctt cgcgccgacc gctggatcgg ctatccgcgc gcagtcgagg cgctgaaccg
     1141 gctggaagcc ctttatgcgt ggccaaacaa gcaacgcatg cccaacctgc tgctggttgg
     1201 cccgaccaac aatggcaagt cgatgatcgt cgagaagttc cgccgcaccc acccggccag
     1261 ctccgacgcc gaccaggagc acatcccggt gttggtcgtg cagatgccgt ccgagccgtc
     1321 cgtgatccgc ttctacgtcg cgctgctcgc cgcgatgggc gcgccgctgc gcccacgccc
     1381 acggttgccg gaaatggagc aactggctct ggcactgctg cgcaaggtcg gcgtgcgcat
     1441 gctggtgatc gacgagctgc acaacgtgct ggccggcaac agcgtcaacc gccgggaatt
     1501 cctcaacctg ctgcgcttcc tcggcaacga actgcgcatc ccgttggttg gggtaggcac
     1561 gcgcgacgcc tacctagcca tccgctccga tgaccagttg gaaaatcgct tcgagccgat
     1621 gatgctgccg gtatgggagg ccaacgacga ttgctgctca ctgctggcca gcttcgccgc
     1681 ttcgctcccg ctgcgccggc cttccccaat tgccacgctg gacatggctc gctacctgct
     1741 cacacgcagc gagggcacca taggggaact ggcgcacttg ctgatggcgg cggccatcgt
     1801 cgccgtggag agcggcgagg aagcgatcaa ccatcgcaca ctcagcatgg cctgttgagt
     1861 tgcatctaaa attgacccac ttagggtaaa gatttgcgtc gaaatttgac ccacgtatga
     1921 cactgtttcc cgtctggata tggcgggaga aatcaaggag tgataaacgt ggcgatattg
     1981 agcgcaattc gacgctggca ttttcgcgat ggtgcgtcga ttcgggaaat agcccgacga
     2041 agcggcctgt ccaggaacac cgttcgcaag tatttgcaaa gcaaggtggt tgaaccgcag
     2101 tacccagcgc gagacagcgt tggcaagtta agtccttttg agcccaagtt aaggcagtgg
     2161 ctctccaccg agcacaaaaa gacaaagaag ctgcgcagaa acctgcgcag catgtaccgg
     2221 gatttggtcg ctttgggctt taccgggtct tatgaccgag tgtgtgcctt tgcccgacag
     2281 tggaaagatt ccgaacagtt caaggcgcaa acctcgggca agggttgttt catccccttg
     2341 cgctttgctt gtggcgaagc cttccaattc gattggagtg aggactttgc ccgcatagcg
     2401 ggcaaacagg tcaaacttca gattgcccag tttaagttgg cccacagccg ggcctttgtg
     2461 cttcgggctt actaccagca aaaacatgaa atgctgtttg atgcccactg gcatgccttt
     2521 caaatcttcg gtggcattcc caagcgcggc atctacgaca acatgaagac cgctgtggat
     2581 tcggtggggc gtggcaaaga gcgcagggtc aatcagcggt tcactgccat ggtcagccac
     2641 tacctgtttg atgcgcagtt ctgtaatcca gcatcgggtt gggagaaagg ccagattgag
     2701 aagaacgtgc aggattcccg ccaacgcctg tggcaagggg caccagactt tcaaagcctt
     2761 gctgatttga atgtgtggct tgagcatcgc tgcaaagcgc tgtggtctga gctgcgccac
     2821 cccgaattgg accaaaccgt gcaagaggcc tttgccgatg aacaaggcga gttgatggcg
     2881 ctacccaatg cctttgatgc attcgtggag caaaccaagc gagtcacttc aacctgcctt
     2941 gttcaccacg agggcaatcg ctacagcgtt cctgccagtt acgccaacag ggccatcagc
     3001 cttcggattt atgcagacaa gctggtgatg gctgccgaag gccaacacat tgccgagcat
     3061 ccaagattgt ttggcagtgg ccacgctcgg cgtggccaca cacaatacga ctggcaccat
     3121 tacttgtctg tgcttcagaa gaaacctggg gcgttgcgca atggtgcgcc atttgctgaa
     3181 ttgccacccg cgttcaagaa gcttcaatcc atcttgctgc aacgccccgg cggtgaccgt
     3241 gacatggtgg aaattctggc ccttgtattg caccacgatg aaggtgcggt actcagtgct
     3301 gtggaattgg cattggagtg tggcaagcca tcgaaggagc atgtgcttaa tctgttggga
     3361 cgtttgaccg aagaacctcc acccaaaccg attccaattc ccaaggggtt aaggctgaca
     3421 ttggaaccac aggccaacgt gaaccgctat gacagtttaa ggagagccca tgatgcagca
     3481 tgaaggccat gtgagaatcc tcaaatcctt gaaactcttt ggcatggcac acgccattga
     3541 ggagttgggc aatcagaatt caccagcatt taatcaagcc ttgcccatgc tggacagctt
     3601 gattaaagct gaagtggcag agcgtgaagt acgttcggtg aactatcaat tgcgggtggc
     3661 caagttcccc gtgtatcggg acttggtggg ctttgacttc agtcaaagcc tggttaatga
     3721 ggccacggtc aaacaattgc accggtgcga cttcatggaa caagcccaga acgtggtgct
     3781 gattggtggg ccaggcacag gcaagactca cctggccaca gccattggta cacaagcagt
     3841 gatgcacttg aaccgacggg tgcgtttctt ctccaccgtg gatttggtca atgcactgga
     3901 gcaagagaaa tcatctgggc gtcagggaca aatcgcaaac cgtctgttgt atgccgattt
     3961 ggtgattctg gatgagctgg gatatttgcc ttttagccaa accggtgggg cactgctgtt
     4021 tcacctgctc tcaaagctgt acgaaaaaac cagcgtgata ctgaccacca acttgagctt
     4081 ctcggaatgg agccgagtgt ttggcgatga aaagatgaca acagcgttgt tggaccgact
     4141 aacccaccac tgccacatcc tggaaaccgg caatgaaagt taccgcttca aacacagttc
     4201 aactcagaat aagcaggagg aaaaacagac ccgcaaactg aaaatcgaga cataattctg
     4261 acaacaaggg gtgggtcaaa attcaatgca aatcccgggt caaatttggg tgcaaatcaa
     4321 cagatatcga caacctctcg cgcaaccaag acatcgcggt cggactgcaa gtgatcttga
     4381 agccacgggc ccgtcccacc ccgacatgga cctcgatgcc cgaacggacg ttagatttcg
     4441 agttctaggc gttctgcgat gaaggttgga tcccagccgg gattgaaagt gtcgacgtgg
     4501 gtgaatccga gccgctcgta taggccacgc aggttcgggt ggcagtcgag ccgcagcttg
     4561 gcgcacccct gcgttcgcgc ggcatggcgg caagcctcga tcagcgcgga
//
LOCUS       DAJOWB010000226         4558 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJOWB010000226 DAJOWB010000000
VERSION     DAJOWB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.227"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(217..630)
                     /locus_tag="OHO85_004700"
                     /pseudo
     CDS             complement(217..630)
                     /locus_tag="OHO85_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
     gene            628..885
                     /locus_tag="OHO85_004701"
                     /pseudo
     CDS             628..885
                     /locus_tag="OHO85_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(1119..1976)
                     /gene="sitD"
                     /locus_tag="OHO85_004702"
     CDS             complement(1119..1976)
                     /gene="sitD"
                     /locus_tag="OHO85_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCQ2673944.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKSWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQASGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISILVSMTGVWLSFFIDSAPAPTIVVLFAVMFIMTFTVTSINARTKENA
                     EPRDLLSSD"
     gene            complement(1973..2830)
                     /gene="sitC"
                     /locus_tag="OHO85_004703"
     CDS             complement(1973..2830)
                     /gene="sitC"
                     /locus_tag="OHO85_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCQ2673945.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQLTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2827..3654)
                     /gene="sitB"
                     /locus_tag="OHO85_004704"
     CDS             complement(2827..3654)
                     /gene="sitB"
                     /locus_tag="OHO85_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCQ2673946.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPGGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(3654..4544)
                     /gene="sitA"
                     /locus_tag="OHO85_004705"
     CDS             complement(3654..4544)
                     /gene="sitA"
                     /locus_tag="OHO85_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCQ2673947.1"
                     /translation="MLLGCLVLTCSIAFQASAAEKFKVITTFTIIADMAKNVAGDAAE
                     VSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSS
                     GVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDAFIKYDPANAQTYQRNADTYKA
                     KITQTLAPLRKQIAELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQV
                     RKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYID
                     LLKVTTSTLVQGIKAGKREK"
BASE COUNT         1232 a         1185 c         1066 g         1075 t
ORIGIN      
        1 ataccctcga caattgctga gcagaagccc ccacgacaag tgacagaatg acaacatcta
       61 cagtggggag cgttttatcg atgaacaagc aaccaagaag taacacggca taggtgacga
      121 caatgccagt aagcatagaa ataacaaaaa cgtgagcttt catcattcct ccagaataac
      181 cccgacgtgt aagcctgggg gattggcaca ataatgtcat ttaaaggcag actttccggc
      241 aaattgcgcc gatgaaccca gttcatcctc aattctcata agctggttat atttttcaat
      301 acgctcaccc cggcatggag ctcccgtttt gagatgacct gcgcgcattg caactgtcat
      361 atcggcgata aagctgtcca ctgtctcacc actgcggtgt gagataaagg tgccccagtt
      421 gttgtcatgg caaagttgta ccgcatcaaa ggtttcactc agggaaccaa tctggttgag
      481 tttaatcaat gctgaattgg caaggttttc gtcaatgccg cgctgaatat actttacgtt
      541 ggtcacaaac agatcgtcgc caaccaattc tatctgctct cccaatgcac catgcaggat
      601 tttccatcca gcccagtcat cttcagctac cgttaagggc aggaagatca actaggtgct
      661 ctggggctgg tgaccaatgc ggttgtgctg tgaaacacac tttacatgca ggaggcgttg
      721 tcacatcttc gcaaatcggg agaaactcct gaggagtagc atccggcaag attatcgctg
      781 ctgatacata gccatataaa tatgctgggg cattatacgt cttcgttacc ggaagatatt
      841 cttaagggtg agttaagatc attaaacttc aatacaaaca attaattaaa ctcttagcgt
      901 ggttttttac atcactggcc ctcgcccccc aattttctct atgttttgcc actacttccc
      961 tcattttact gcgctgtttg ctacccctga gaatcgacat tacttgctat ccagtccgca
     1021 gcaactctct ggcaacaaaa cttatcagaa tcataagtct gtgcattatt cgttcaagcg
     1081 catcgccaat aagcggatgt tgctggccga gtgttctgct aatcggatga taacaaatcc
     1141 cgtggttcgg cattttcctt tgtgcgtgcg ttgatgctgg taacggtaaa cgtcatgatg
     1201 aacatgaccg cgaataagac aacaattgtc ggagctggcg cgctgtcgat aaaaaaggac
     1261 agccagacac cagtcattga gaccaatatc gatatgccag tcgccagcag gagcgcaata
     1321 tgaaagcgtt gtgtcaggag taccgcaatg gcaccaggcg caatgagaag agaaatagag
     1381 agaatgatac ctaccgcttt cagcgtcgcc acaatggtca gggagaccat gcacagcagc
     1441 ccataatgca gccatctcgt atgcaaaccg cttgcctgcg cctgttgata atcaaagctg
     1501 aacagcaaaa aatcccgcca ttttacgcta ataacaagtg taaccagtcc ggcaataatc
     1561 accgtctgga ttatatcgcc gatggttatc ccgagcatgt cgccgaaaag aatatgatca
     1621 agatgtacgt ctggtttgac tgcaatatac aagatcagac ctgcggcaaa catgccagaa
     1681 aaaacaatcc ccatcactgt atcttgcttg attcggctgt tgtctttcag gtatcccgtc
     1741 gccaccgcac agaatactcc ggcgacgaat gcccctgtag ccagaggtaa ccccagaatc
     1801 caggcaagca cgatgccagg aaaaaccgca tgactcatcg cgtcacccat cagtgcccaa
     1861 cttttcagga ccagaaagac cgacagcagc gcacagggta tggaaacaac cagtgaaatc
     1921 agcagtgcgt gactcataaa ggtgaactgc agtggctcca gtaataatgc catcatgagc
     1981 gatcctccag cgctttatgc gcacgccgac gatttgccaa aagcccatgg gtgggcgcaa
     2041 agacaaatgc taaaagaaac agcagtgtct gcgccaccac gatgattcca cctgtcgcgc
     2101 cgtccagaaa gtaactgacc cacgcgccga ggaaactggt cacactgcca atagttacag
     2161 caatcatgag caggcgggga aaacgatcgg ttaacaacca tgctgttgca ccgggagtca
     2221 ccaccagaca gatcaccaga aaggcaccaa ccgtttgcag cgccgcaacg gtcgaaaccg
     2281 acagcagggt gaagaagagg atttttaatc tgcccggatg caatccaata gcacgggcgt
     2341 gattctcgtc gaaaaaggtc accataagat ctttccattt gaaaaaaagc acgatgatcg
     2401 agagaatgcc aatgatagtg agttgcagga tatccgccgg atcgattgcc agaatattgc
     2461 ccaggacgat ggtctgaatg ttcacggatg tcgggttcaa tgacaccata aacagcccca
     2521 ggccaaaaaa ggacgagaag atcaggccga tgatggcatc ttcttttaag cgagtgagtt
     2581 ggttaagaaa cagcatgctg cccgctgcca gtccgccgga aaagaaggct cccagagaaa
     2641 acggaagccc cagcatatat gcccccgcaa cacccgggac aatagagtgc gacagcgcat
     2701 cgccaatcag cgaccagcct tttagcatca gatagcatga caggaaagcg cagagaccgc
     2761 cgaccatcgc cgagacccac atcgcattga gcatatattc atagctgaag ggttccagta
     2821 gcacgttcat ctttcttccc tctgcaccgc tgacggtcga tgtgccacaa aagggcgttc
     2881 atcgtcagta ataatgcttt cttccgagcc gttaagtgtg acatggcgca gtacgccgct
     2941 aaaagccagc tccaggttct cggctgtaaa agtggtgtct gtcggcccgc ttgccaggac
     3001 ggtgcctttg accatgaccg tataatcgca aaatgtcgtg acggatccga gattgtgcgt
     3061 tgagacaagc atcgttttgc cttccgcgcg taactccctc aacaggctga tgattttcgc
     3121 ttcggttttc acatcaacgc cggtaaaagg ttcatccagc aggatcacat caccctgctg
     3181 tgcaatcgct ctcgccagaa agacgcgttt cttttgtcca ccggaaagct cgccgatttg
     3241 tcgatggcga aaatccacca tatcgacacg ttccagcgca tcggtgacaa tctgacgatc
     3301 cctctttttg gcgatacgca acatacccat atgcccatag cggcccatca tcaccacatc
     3361 ttccaccagt acaggaaatg accagtcaac ctcttctgac tgagggacgt aggcaaccag
     3421 gtttttctgt agcgcctgtc gtgtgggaat acccagaaca gatatcttcc cgctggtcag
     3481 acgcacaaat cccataatcg ctttaaacaa cgtcgatttt ccggaaccgt ttaccccaac
     3541 cagagcggca atagatccgc ctggtactgt gaaggatgca tcgcgcagtg ctgtgtgccc
     3601 gttacgccag gtgacagtaa catcattaac gacaatgcct gcagattgca tcattatttc
     3661 tccctttttc cggctttaat tccctgtacc agcgtactgg tagtaacctt cagaaggtcg
     3721 atatacgtag gtaccgggcc gttttctgtg ctcagggaat cgacatagag cacaccaccg
     3781 tagtgcgcgc cggtttcacg cgcaacctga cgcgctggtt tatcggaaat cgtactctcg
     3841 ctaaagactg ccgggatatg atttttctta actatatcaa caaccttacg tacctgctgc
     3901 ggtgttcctt gttgatcggc attaatcggc cacagataaa gctcttttag ccccaaatcg
     3961 cgtgcgagat aagaaaaagc cccttcactg gtgaccatcc atcgctgatt ctcagggagt
     4021 tccgcaatct gcttacgcag gggggcaagg gtttgggtaa tcttggcttt ataagtatcg
     4081 gcattacgtt ggtaggtttg tgcatttgcc ggatcgtatt ttataaacgc atcacgaata
     4141 ttatcgacgt aaatcagagc attatctggc gacatccagg catgggggtt aggtttgccc
     4201 tcatagggcc cttcggtgat ccctactggc gtcacacccg aagagacaat tacttctgga
     4261 accccattga gatgctggta aaagcgttgg aaccacaatt ccagattcat accattggcg
     4321 agaatcagtt gtgccccctg cgcacgttta atatcgccag gggtaggctg atactcatga
     4381 atttctgcac caggcttggt tatggatgag acttctgcag catctccagc cacgtttttg
     4441 gccatatctg cgatgatggt aaaagttgta atgaccttga atttttcagc tgcactcgcc
     4501 tgaaatgcta tcgagcaggt gaggactagg caccccaaga gcatggttac ttttttta
//
LOCUS       DAJOWB010000227         4540 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJOWB010000227 DAJOWB010000000
VERSION     DAJOWB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.228"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..449
                     /locus_tag="OHO85_004706"
     CDS             <1..449
                     /locus_tag="OHO85_004706"
                     /inference="COORDINATES: protein motif:HMM:NF013579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCQ2673948.1"
                     /translation="TLKKGDVIITKDSETPGDIAIPALVSEDLNGVVCGYHLAIIRPK
                     NKIVVGSFLNYLFSMQKTRYYFFTLATGATRFGLSVGGINYAHFSIPKIEEQQKIAAV
                     LSAADAEISVLEKKLACLKDEKKALMQQLLTGKRRVKTEVEETVSA"
     gene            470..3754
                     /locus_tag="OHO85_004707"
     CDS             470..3754
                     /locus_tag="OHO85_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCQ2673949.1"
                     /translation="MNQSYTPKFQEEYSAKIPALTLLTSLGWTFLSPKQIMDYRGYKQ
                     DEVVLRPILREVLSKRSFMAGGKTCRLSEKALDNLLSQVCSPALNEGLLKANERMYNH
                     LLYGIAVTEFVDGKKVNPTIALIDWEHPQNNQFHFAEEFSVLRSGGVETRRPDIVCFV
                     NGIPLAVIEAKSPVGHGKKGPTIDEGISQSIRNQLNDEIPQLFAYSQLLLSINGHDGR
                     YGTCHTPMKFWAAWREEDITDAQMYAIRNHPLSTEQIDALFAHRPPADRNWYQQLIAA
                     GELALSGQDKLLISLLSPERLLEMTRFFTLFDKKNGKIVARYQQVFGIKRLLERISTR
                     RPDGGREGGVIWHTTGSGKSYTMVFLSKALILHDSLKQCRIVVVTDRIDLEEQLSGTF
                     ASGGELAGKKDKANAMATSGQMLAKQIGSGKERIIFTLIQKFNSATKLPECVNTSPDI
                     IVLIDEGHRSQGGENHVRMKLALPNAAFVAFTGTPLLKKDKTTNKFGPIVHAYTMQRA
                     VEDQAVTPLLYEERIPDLEVNDRAIDAWFDRITDGLSDAQKADLKRKYARKGEVYSAD
                     DRIRLIALDIATHFSKNIDEGLKGQLACDSKISAIKYKKYLDEAGLFESAVVISPPDT
                     REGNTEVDESKLPEVTKWWKDNVGTQDESAYTRDIISRFDTDDKLKLLIVVDKLLTGF
                     DEPKNTVLYIDKPLKSHNLIQAIARVNRLHPLKKFGLLIDYRGILAELDTTIGKYQDL
                     ASRTQGGYDIKDIDGLYSAMISEYKRLPHLYNQLWAIFAGVKNKNDTEQLRAVLVPKM
                     EERDGEMLDIHQKTRDDFFEALTAFAGCLKVALQSATFFTDKSFTEQDRNLYKETVKQ
                     MSSLRQWAMQVSGEQVNYDDYAEQVKKLLDKHVTGVAVREPDGVYEVGKMGKTEKPEE
                     WDNNKTRNETDIIKTRVTKMIEHELCDDPYAQEAFSKLLRMVIEETEKLFDHPLKQYL
                     LFREFEAQVEARKLSDIPDALAVNKHAQAYYGVFKKELPEVFAVNDDQVQHKWTKLAF
                     EVDTIIVKAVAENSLNPQDIEKAVKTSLLPRLFTACREIGAGMNQVNRIVETIIQILR
                     VGLMKS"
     gene            3751..4506
                     /locus_tag="OHO85_004708"
     CDS             3751..4506
                     /locus_tag="OHO85_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCQ2673950.1"
                     /translation="MSEKKSLSMNAVRFVYGDETIVVQCVPRKVVKGRILIKVHPDCR
                     VVASVPPGTPEQEVLSALKKRGRWIYQQLRDFREQQTHIVPRQYVSGESHYYLGKQYQ
                     LKVTEDATVPQRVKMLRGRLEVTVRQKSAERVKALLTEWYRERARDVFQRRLDLLVPQ
                     TLWVSERPPIRLRAMQTQWGNCSAKGCLTLNPWLVKATSECIDYVLLHELCHVAEHNH
                     SEEFYRLMGQVMPGWEKVKKRLDGMAGMLLADL"
BASE COUNT         1260 a         1052 c         1182 g         1046 t
ORIGIN      
        1 atacacttaa aaaaggtgat gtaattataa ccaaagattc tgaaacgcca ggcgatatcg
       61 ctatccctgc tttggtttca gaagatttaa atggtgtagt ttgcggttac cacctcgcaa
      121 taattcgccc gaaaaataaa attgttgttg gttcattctt gaattatctt ttttctatgc
      181 agaaaactcg ctactacttc ttcaccttag caacgggcgc aacaagattt ggcttatcag
      241 ttggtggcat aaattatgct cacttcagta ttccaaaaat agaagaacag caaaaaatcg
      301 ctgccgttct gtccgcagcc gacgccgaaa tctccgtgct ggagaaaaaa ctcgcttgcc
      361 tgaaagatga gaaaaaagcc ctgatgcagc agttactgac cgggaaacgc cgtgtcaaaa
      421 cagaagttga agaaaccgtt agcgcataac aggcgaacaa ggaacgctca tgaatcagtc
      481 gtacacacca aaattccagg aagaatacag cgccaaaatc ccggcgctga cgttactgac
      541 cagcctcggc tggacgtttt tatccccgaa acagattatg gattaccggg gctataaaca
      601 ggatgaggtg gtgctacgcc cgattctgcg tgaagtgttg tcgaagcgtt cctttatggc
      661 gggcggtaaa acctgccgct tatcggaaaa agcgttggat aacctgctat cgcaggtctg
      721 ttccccggcg ctgaacgaag ggctgctgaa agccaatgaa cggatgtata atcatctgct
      781 ctacggtatc gccgtcacgg agtttgtcga cggcaagaaa gtgaacccca ccattgcgct
      841 gattgactgg gaacacccgc aaaataacca gttccacttt gccgaggagt tctcggtgct
      901 gcgctcaggt ggtgttgaaa cccgcagacc ggatatcgtc tgttttgtga atggtatccc
      961 gctggcggtt atcgaggcga aaagcccggt cggccacggt aaaaaggggc caaccattga
     1021 cgaggggata tcacagagta tccgcaatca gctcaacgat gaaatcccac aactgtttgc
     1081 ctacagccag ttgctgctgt ctattaacgg gcatgacgga cgctatggca cttgtcatac
     1141 gccgatgaaa ttctgggcag catggcgtga agaagatatt accgatgcgc agatgtacgc
     1201 catccgcaat catccgctat ctactgagca aattgatgcg ttgtttgccc atcgcccgcc
     1261 tgccgatcgc aactggtatc agcagttaat tgctgccggt gaactggccc tcagcgggca
     1321 ggacaaactg ctgatcagtc tgttgtcgcc agagcgtctg ttggagatga cccgcttctt
     1381 taccctgttc gacaaaaaga acggcaaaat tgtcgcccgc tatcagcagg tattcggtat
     1441 taaaagactg ctggaacgca tcagtacccg cagaccagac ggcggcaggg aaggtggtgt
     1501 catctggcat accacgggtt ccggcaaatc ttacaccatg gtgtttctca gtaaggcgct
     1561 gattctacat gacagcctga agcagtgccg tatcgtggtg gtgacagatc gcatagatct
     1621 tgaggaacag ctcagcggta cctttgcgtc cggcggagaa ttggccggga aaaaagacaa
     1681 agctaatgca atggcgacgt ccgggcagat gctggcgaaa cagatcggct ccggcaaaga
     1741 acgtattatc ttcaccctga tacagaaatt taactcagcg acaaaactgc ctgaatgcgt
     1801 taataccagc cccgatatta tcgtcctgat tgatgaaggg caccgcagtc agggtggcga
     1861 aaaccatgtg cggatgaaac tggcgctgcc gaatgccgcc tttgtggcat tcaccggcac
     1921 gccgctgctg aaaaaagata aaaccaccaa taaatttggc ccgatcgtcc atgcttatac
     1981 catgcaacgg gcggtggaag atcaggccgt tacaccgctt ctctatgaag aacgtattcc
     2041 ggatcttgag gtgaatgacc gcgcgattga cgcctggttt gaccgcatta ccgatggtct
     2101 gagcgatgcg caaaaggctg acctcaagcg taagtatgcc cgtaaaggtg aggtttacag
     2161 cgcagatgac cgtattcgcc tgattgcgct ggatatcgcc acacacttct cgaaaaatat
     2221 tgatgagggt ctgaaaggac aacttgcctg cgacagcaaa atctctgcca ttaaatataa
     2281 aaagtatctt gatgaagccg gtttgtttga atcagcggtg gtgataagcc ccccggatac
     2341 ccgggaaggg aataccgagg tggatgaaag taagctgccg gaagtaacga aatggtggaa
     2401 ggataatgtc ggtacgcagg atgaatccgc atacacccgc gatatcatca gtcgtttcga
     2461 taccgatgat aagcttaaat tgctgattgt cgtcgataaa ctgctcaccg ggtttgatga
     2521 accgaagaat accgtgctgt atatcgacaa accgcttaaa tcccataatc ttattcaggc
     2581 tattgcccgg gtaaaccgac tgcatccact gaaaaagttt ggcctgctga ttgattatcg
     2641 cggcattctg gctgaactgg acacgacgat cggtaaatat caggatctcg cctcccgaac
     2701 tcagggagga tacgacatca aggatattga cgggttgtac agcgccatga tttctgaata
     2761 taaacgtctg ccgcatctgt ataaccagtt gtgggccata tttgccggtg ttaaaaacaa
     2821 aaatgatact gagcagttac gtgcagtact ggtgcctaaa atggaagaac gtgacggaga
     2881 gatgctggat attcatcaga aaactcgcga tgacttcttt gaagcgttaa ccgcttttgc
     2941 tggatgcctg aaagtagcgc tgcagtccgc caccttcttc accgacaaga gctttacgga
     3001 acaagaccga aatctctata aagaaaccgt aaaacagatg tccagcctgc gccagtgggc
     3061 gatgcaggtc agtggtgagc aggtcaatta tgacgactac gctgaacagg tgaaaaaact
     3121 gctggataag catgttactg gtgtcgcggt tcgtgaaccg gatggcgtat atgaagtggg
     3181 caagatgggt aaaactgaaa agccggaaga gtgggataac aacaaaaccc gcaatgagac
     3241 cgacatcatt aaaacccgcg tcacgaagat gattgaacat gaactgtgcg acgatcccta
     3301 tgctcaggag gcattctcaa aactcctgcg tatggttatt gaagagactg aaaaactgtt
     3361 cgaccatccg ctgaagcaat atctcctttt ccgcgagttt gaggcgcagg tggaagcccg
     3421 caaactcagc gacatcccgg atgcgctggc cgtaaacaaa catgcgcaag catactacgg
     3481 agtatttaaa aaagagctac ctgaggtttt cgcggtaaac gatgatcagg ttcagcataa
     3541 atggacgaag ctggctttcg aagtggacac tattattgtc aaagcggttg cggaaaattc
     3601 cctcaatcct caggatattg agaaagccgt taagacaagc ctgttgccgc ggctgtttac
     3661 tgcctgtcga gagatcggtg ctggaatgaa ccaggtaaac cgtatcgttg aaaccatcat
     3721 ccagatcctg cgcgttggtt tgatgaaatc atgagtgaga aaaagtcact atctatgaat
     3781 gctgtacgtt ttgtttatgg tgatgaaacc atcgtggttc agtgtgtccc acggaaggtt
     3841 gttaaaggca ggatacttat caaggttcat ccggactgcc gggtagtcgc atctgtacca
     3901 cccggaacgc cggagcagga agtgctgtct gcgctgaaaa aacgagggcg ctggatatac
     3961 cagcaactgc gcgatttcag agaacaacag acgcatatcg tcccgcgcca gtacgtgagt
     4021 ggcgaaagcc attactatct tggtaagcaa taccagttga aagtcactga agacgctact
     4081 gttccgcagc gagtaaaaat gctgcgggga cgtctggaag tcacagtcag acaaaaatct
     4141 gcggaacgcg tgaaggcgtt gcttactgag tggtatcggg aacgtgccag agatgttttt
     4201 caacgcagac tggatttatt ggtaccgcaa accttatggg taagtgagcg tccgccgatc
     4261 cgtttgcgag ccatgcaaac acaatgggga aattgctctg caaaaggttg cctgacactt
     4321 aacccatggc tggtaaaagc gacctcagaa tgcatcgact atgtattgct gcacgagtta
     4381 tgtcacgtcg ctgagcacaa tcacagcgag gaattttatc ggctaatggg gcaggttatg
     4441 cccggatggg aaaaggtaaa aaaacgcctg gacgggatgg ccgggatgct gttggctgat
     4501 ctgtagtttg ttacttaccg ttccatgaat tgccggtgat
//
LOCUS       DAJOWB010000228         4477 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJOWB010000228 DAJOWB010000000
VERSION     DAJOWB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.229"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1227
                     /gene="ag43"
                     /locus_tag="OHO85_004709"
     CDS             <1..1227
                     /gene="ag43"
                     /locus_tag="OHO85_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCQ2673951.1"
                     /translation="GRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAF
                     VQGNRLQAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRIVAGSRSH
                     QTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLMRTEVAGMS
                     VTAGVYGAAGHSSVDVKDDDGSRVGTVRDDAGSLGGYLNLIHNASGLWADIVALGTRH
                     SMKASTDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGKDNAGY
                     VKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVSELPVNWWVQPSVIR
                     TFSSRGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSVSG
                     SSAEGYNGQATLNVTF"
     gene            1348..3864
                     /locus_tag="OHO85_004710"
     CDS             1348..3864
                     /locus_tag="OHO85_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673952.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIVEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRLALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            3940..4395
                     /locus_tag="OHO85_004711"
     CDS             3940..4395
                     /locus_tag="OHO85_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673953.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
BASE COUNT         1069 a         1150 c         1233 g         1025 t
ORIGIN      
        1 ggcagggcaa ccggaaaaac catcctgaac ctggtgaacg ccggcaacag tgcgtcgggg
       61 ctggcgacca gcggtaaggg tattcaggtg gttgaagcca ttaacggtgc caccacggag
      121 gaaggggcct ttgtccaggg gaacaggctg caggccggtg cctttaacta ctccctcaac
      181 cgggacagtg atgagagctg gtatctgcgc agtgaaaatg cttatcgtgc agaagtcccc
      241 ctgtatgcct ccatgctgac acaggcaatg gactatgacc ggattgtggc aggctcccgc
      301 agccatcaga ccggtgtaaa tggtgaaaac aacagcgtcc gtctcagcat tcagggcggt
      361 catctcggtc acgataacaa tggcggtatt gcccgtgggg ccacgccgga aagcagcggc
      421 agctatggat tcgtccgtct ggagggtgac ctgatgagaa cagaggttgc cggtatgtct
      481 gtgaccgcgg gggtatatgg tgctgctggc cattcttccg ttgatgttaa ggatgatgac
      541 ggctcccgtg tcggcacggt ccgggatgat gccggcagcc tgggcggata cctgaatctg
      601 atacacaacg cctccggact gtgggctgac attgtggccc tgggaacccg ccacagcatg
      661 aaagcgtcaa cggacaataa cgacttccgc gcccggggtt ggggctggct gggttcactg
      721 gaaaccggtc tgcccttcag tatcactgac aacctgatgc tggagccaca actgcagtat
      781 acctggcagg gactttccct ggatgacggt aaggacaacg ccggttatgt gaagttcggg
      841 catggcagtg cacaacatgt gcgtgccggt ttccgtctgg gcagccacaa cgatatgacc
      901 tttggcgaag gcacctcatc ccgtgccccc ctgcgtgaca gtgcaaaaca cagtgtgagt
      961 gaattaccgg tgaactggtg ggtacagcct tctgttatcc gcaccttcag ctcccgggga
     1021 gatatgcgtg tggggacttc cactgcaggc agcgggatga cgttctctcc ctcacagaat
     1081 ggcacatcac tggacctgca ggccggactg gaagcccgtg tccgggaaaa tatcaccctg
     1141 ggcgttcagg ccggttatgc ccacagcgtc agcggcagca gcgctgaagg gtataacggt
     1201 caggccacac tgaatgtgac cttctgacag aaccatcgcc tctctgtggt cccggtcatc
     1261 atgaccggga cccggaccgg cgcaacggat cttcaacgcc acattcgctg gcattaacaa
     1321 taacatgata ttcatcacgg agtgactatg ttacagatag tcggcgcgct gatcctgctg
     1381 atcgcaggat ttgccattct tcgccttttg ttcagagcat taatcagcac ggcttctgcg
     1441 ctggcagggc tcatattgct gtgtctgttc ggcccggcct tactggctgg ctatatcacc
     1501 gaacgcataa cccggttgtt ccatattcgc tggctggcag gcgtatttct gacgattgcc
     1561 ggaatgatca tcagcttcat gtggggactt gatggtaaac atatcgcgct ggaggctcac
     1621 acctttgact ctgtgaaatt tattctgacc accgctctcg ccggtggtct gctggctgtt
     1681 cccctgcaga tcaaaaacat tcagcagaac gggatcacac cagaagatat cagcaaggaa
     1741 attaacgggt attactgctg tttttatact gcctttttcc ttatggcgtg ttctgcatgc
     1801 gcaccattga tcgcgttaca gtacgatatt tcaccgtcac tgatgtggtg gggcgggtta
     1861 ttgtactggc tggctgcatt agtgacgctg ctatgggcgg ccagccagat ccaggcgctg
     1921 aaaaaactga cctgtgccat cagccagaca ctggaagaac aaccggtgct caacagtaaa
     1981 tcgtggctga ccagtttgca aaacgattac agccttcctg actcactgac ggagcgcatc
     2041 tggctgacgc tcatttctca acggatttcc cggggagagc tgagggaatt tgaactggca
     2101 gacggaaact ggttactgaa caatgcctgg tatgaaagaa acatggcagg gtttaacgaa
     2161 cagttgaaag agaacctgtc attcacacct gatgaactga aaacgctctt ccggaaccgc
     2221 ctgaatttat caccggaagc gaatgacgat tttctcgatc gttgcctgga cggcggtgac
     2281 tggtatccct tttcagaagg tcgccgtttt gtatcattcc atcacgtgga tgagcttcgt
     2341 atctgtgcct cctgcgggct gacagaagta catcatgccc cggaaaatca taagccggat
     2401 ccggaatggt actgctcctc tctttgtcgc gaaacagaaa cactgtgtca ggaaatttat
     2461 gaacgccctt acaacagctt tatttccgat gcaacggcga atggtctgat tctcatgaaa
     2521 ctgccggaaa cctggagtac aaatgagaaa atgtttgctt ccggagggca gggacatggg
     2581 tttgccgctg aacggggaaa ccatattgtc gacagagtcc gtctgaaaaa cgcacggatc
     2641 ctcggtgata ataatgccag aaatggagca gacagactgg tcagcggaac agaaatccag
     2701 acgaaatatt gttcaactgc agcccgtagc gtcggtgcgg cattcgacgg acaaaacgga
     2761 cagtatcgtt acatgggaaa taatggcccc atgcaactgg aagtcccccg tgatcagtat
     2821 gccggcgctg ttgaaaccat gaggaataag atccgcgaag gtaaagtacc cggtgtaacc
     2881 gatcccgcag aagcgtcccg gctgattcgt cggggacatc tgacttatac tcaggcccgt
     2941 aatatcaccc ggttcgggac catcgaatcg gtcacttatg atattgtcga gggttcggtt
     3001 gtcagtctgg cggccggagg gatcagtttt gccctgacgg catcggtctt ctggctcagc
     3061 accggcgatc gcgatgctgc cctgcagaca gctgctgtcc aggcaggaaa aaccttcacc
     3121 cgcacactgg ctgtctacgt cacaacccag caactccacc ggctcagtgt tgttcagggt
     3181 atgctgaagc acattgattt ttcgactgcc agcccgactg tccgactggc tcttcagaag
     3241 gggaccggtg caggaaatat cagtgccctg aacaaagtaa tgaagggtac gctggtgaca
     3301 tctctggcac tggtagctgt cacaaccggc cctgacatga tcaaaatgtt gcggggacgg
     3361 atctccggtg cgcagttcat caggaatctt gccgtggcat cttccggtgt ggcaggtgga
     3421 gctgtcgggt cagtggcggg cgggatactg ttcagtccac tgggaccatt tggtgcactg
     3481 acagggcgtg tggttggcgg tgttctgggg ggaatgattg cctccgctgt atcaggaaaa
     3541 attgccggag cgctggttga agaagatcgc gtcaaaattc tggcaatgat tcaggagcag
     3601 gtgacatggc ttgccggcag tttcctgctg accggacatg agattgaaaa tctgaacgag
     3661 aatctggccc gtgttatcga tcagaatgct ctggagatca ttttcgccgc cggtatacaa
     3721 caacgggccg cgaccaatat gttaatcaaa ccactggtgg tcagtatcat caggcaacgc
     3781 cccgtcatgg aatatgatgc atcccatctc ggcaatatgg ttaaccgact ggaagaagca
     3841 ttacccccgg agttaccggc ataacggcta ttctgcgcct tttacgcttt atcacctgat
     3901 ttgtcagtgt catcgtcatg actcagagag gtaaatacca tgattcacct gttcaaaacc
     3961 tgcatgatta ccgccttcat tctggggtta acgtggtctg ccccactccg ggcacaggat
     4021 caacgttaca tcagtatacg caatacagat acgatatggc tcccgggaaa tatttgtgct
     4081 taccagttcc ggctggataa tggcggaaac gatgaaggat ttggccccct caccatcact
     4141 ctgcaactca aagacaaata tggtcagacg ctggtgacca gaaaaatgga aacggaagct
     4201 tttggtgaca gtaatgccac gcgaaccaca gacgcatttc tggaaacgga gtgcgtggaa
     4261 aatgtcgcca caaccgaaat cattaaagca actgaagaaa gtaacggcca tcgtgtcagt
     4321 ctgccgttat cggttttcaa tccccaggac taccatccac tgctgattac cgtttccgga
     4381 aaaaacgtta actgatccct gaagtcagtt tcatgcttca gcctctgtca caacgcgtgg
     4441 cagaggcttt ttatttatca ggagaaaaca gatatgc
//
LOCUS       DAJOWB010000229         4382 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJOWB010000229 DAJOWB010000000
VERSION     DAJOWB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.230"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..851
                     /locus_tag="OHO85_004712"
     CDS             <1..851
                     /locus_tag="OHO85_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCQ2673954.1"
                     /translation="YCCADNMDRKPIEDVIFEINKFISLGGRTIVDATGSESIGRDAQ
                     ALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELAATIDKELNQGIGDTDIRAGMI
                     GEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEMGVSP
                     NKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADAVAHL
                     IELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLCIDNP
                     GRLLTA"
     gene            complement(891..1183)
                     /locus_tag="OHO85_004713"
                     /pseudo
     CDS             complement(891..1183)
                     /locus_tag="OHO85_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(1263..2882)
                     /locus_tag="OHO85_004714"
                     /pseudo
     CDS             complement(1263..2882)
                     /locus_tag="OHO85_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3166..3575)
                     /locus_tag="OHO85_004715"
                     /pseudo
     CDS             complement(3166..3575)
                     /locus_tag="OHO85_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238421.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
     gene            complement(3563..3964)
                     /locus_tag="OHO85_004716"
     CDS             complement(3563..3964)
                     /locus_tag="OHO85_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673955.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(4224..>4382)
                     /locus_tag="OHO85_004717"
     CDS             complement(4224..>4382)
                     /locus_tag="OHO85_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCQ2673956.1"
                     /translation="ATLVHFPAWPAVWLERDDDTGFQQVLERADYLAKEHTKAHCTGE
                     RNFGCSRS"
BASE COUNT         1059 a         1182 c         1059 g         1082 t
ORIGIN      
        1 cttattgctg cgctgataat atggaccgca aacctattga agatgtgatc ttcgaaatta
       61 ataaatttat ctcgctcggt ggtcgcacca ttgttgatgc cacagggtct gagtctatcg
      121 gacgagatgc gcaggcatta cgagaagtcg cattaaaaac aggtctgaat attgttgctt
      181 cctccggtcc ctacctggaa aaatttgaaa gccagagaat tcataaaacg gttgatgaac
      241 tggcggcaac gattgataaa gaattgaatc aagggattgg cgatacggat attcgtgccg
      301 gaatgatcgg tgaaataggt gtctcaccga catttaccga agccgagcat aacagcttgc
      361 gggctgcctc gctggcacag attaacaatc ctcatgtggc gatgaatatt cacatgccgg
      421 gctggcttcg tcgcggtgat gaagtactcg acattgtgtt aggtgaaatg ggcgtctcgc
      481 caaataaagt ctctctcgca cactcggatc cgtcaggaaa agacgtggcg tatcagcgga
      541 aaatgcttga taagggtgtc tggctggaat tcgacatgat tggcctagac attaccttcc
      601 cgaaagaggg aatagcgcca ggggtgcagg agactgccga tgccgtcgct catctcattg
      661 agttgggata cgccgatcag cttgttctca gccacgatgt cttccttaaa caaatgtggg
      721 ctaaaaatgg cggtaatggc tggggatttg ttccagatgt ttttctggcc tatctggcgg
      781 agcgcggcgt cgataaaacg atcctcaaaa aactctgtat cgataatccc ggacgattat
      841 tgaccgcata aaaccattat cccccggcac attgcgccgg gggatgtcaa ttaatacccc
      901 gccgttaaat catccaccaa gcgcgagtcg gatgcgccgt acaactcacc atccgaccca
      961 accataatgc tttgcgtgct gcccatcgcc tcattcagcg ccactttctg accttttgca
     1021 tccagcagct tgagcgtatc cggactaaac ccttttcaac acgcagctcg tccggcaacc
     1081 actgatagtg gaaacgaggc gcattggtcg cttcggcaac attcattcca caattgatgc
     1141 tgttcaccac catttgcagc acggtagtga tgatccagct accggtaaca aagtgagtac
     1201 ataaaggcca tactgtcacg aacagtgcaa aagtggctct tccgcaacct gggtgaagat
     1261 tttcatgcca gtggactcat taccctccgc cttagtagtt caagtccggc tcgaccatac
     1321 atctcgcgta tcagcacctt caggcgattt acatgacctt cgacaacacc agtactccag
     1381 cgactgctga ttgcctcaca tatagccgca gcatcagctt ccatccccgc agctacgcac
     1441 cgaagttctg ctgagccgct ttcgtggacc cgagtgaacc agctgcttag ctgtgattta
     1501 ttttaggttt tcagaatacg atagaactcg agtaccagtt gctgcgctat tttcaactcc
     1561 ggttcttttt cacacatcag actaataaat cggaaagcat aattttcttc acccctgatt
     1621 attctccagg gcatcaacca gcggcttacc cgggacactg aaggaagtct tgcggcagta
     1681 gtaaccggtg ggatccagcc tttacgccat ttggcaacag catccctgac gatggtttca
     1741 ctgcctgtaa cccccctggc caccatttcc cgccatatcc ggccggagtt ataattacca
     1801 cattctcgtt gctcttttaa ccactccctc catgggtcca gaagccctcg ctttggaggc
     1861 ctagttgaca tttcagggaa tgttcctgaa cccacccagc gactgactgt cactcgcgac
     1921 aggcctgtaa tacgggatat ttccctgaaa ctacatccct tgttatgcag ggccataacc
     1981 tctgtccaac gctgatgccg ttttttgcgg atgtgctgtt taaggcgttc cagacgacgg
     2041 agcgatgcta cagacacaga tatttctggc tcaggtgatt tcttcagtga taactctctg
     2101 acaacaagac gtatcagagg catatgtctg tacatcattc gttcaggctc atcaccaata
     2161 ttttttagca ggtgccatcg atcggccacc tgtctggcct gaggtgcacc ttcacgtgct
     2221 gctgtcgcat agactccact gcgatcacgc gagacaacct gtatttccgg atattttctg
     2281 aaccaggtcg ccagcgtacg ctgatcacgg ccgggaagca ggacgagggg acggtgagta
     2341 tcaagattga ctattaacat accgcagcag tggccccgat gccacgccca ctcatcaata
     2401 ccgacatgag gcgcgcctga ctgtttcgtc tccggggtat tgataaccct gcgaagaaga
     2461 gtatcagcac tgcaacggag acccgctgcc gttgcagccc gttttccagc ctcacctcca
     2521 gctattaatc ccagttgacg ttgtaaattt tgtaacgcct gtgaagactg ctggtgtgaa
     2581 ccggcgaagg gagcaagcga ctcggcaaaa attttacgtg aacaagccgg gttacggcag
     2641 taccagtggc ggacggaaaa tacaagccat agcgtctgcc ctgagcaggg tagatgctgt
     2701 atccggcgcc gacgacaact atgaacagag tcgctacgct taaagcattc aggacattgt
     2761 gctgttttgc gttttccgtg gagatggagg gttataccgt cagagctaat aatttgtcga
     2821 ctgcatcgcc atccgcaagg taactgtaga agtgtcttaa gagagtgcat tgcgttgccc
     2881 ataacttaaa acagcttaga tccttgttcg ccagaaagtt tcatccattt tcttccaccc
     2941 aggttgcgga agaaccactt ttcatccgtc gttgacagat gtgaagtatg cgggagaaaa
     3001 agtgaatatc taactgtaat aactcagacc ggtacgagta ctcggaacgt ctacaatcat
     3061 tcagggcaaa gtgctaaaat ttctctatcc cgggatgccc ctaccaacgc agcactcccg
     3121 gagatatcat tacagctctg caactgattt ccgcgactga accgctcagt tcctatcctt
     3181 ccagttgcct tcctgcagcc cccctccctg ctgtataccc cggtaccaca acgcgggcag
     3241 cacttttctc cttcataatc ttcgtcacac atcacacaaa accactttct caccttcacc
     3301 gtataatccg gaaccgtcat accctgcaga tgcaaacgac gggcctcttt ctctgccggg
     3361 tcaagccagg tgcatcgcag caggattttc tcagcgacag tcatctggtc atgttcaaac
     3421 cacggtggtt gtccgtcatt gccccgctga agtgttaacg ggcatccaca ggacacacag
     3481 cacaaaacgt cctggcggtt gtgcttcgct gcttctttcg ccgtaacaaa atggcctttg
     3541 ctgtcattcg ccatataaca gttcagaatt cgcatatttc ctcctgagtt gtccggggaa
     3601 tactgaatcc tccggtctgg cagtgtgtgc agtaccgctc cccataataa tcgtggtgac
     3661 attgtctgca gtgccagctg gctttacgca ccacgggtaa ggcatccggt acgaattgct
     3721 gcagacgttt aatcaactgt atttctctgc gctccggcct gacataaggg cactgttgac
     3781 cgtgctctgt cagcctgtcg tcagtgtgtt caaaccaggg aagttcagtg tcgtattgcg
     3841 gatggtatct gagtgcactg ccacacaagt ggcaggtgta gttagcataa ggtgcggcct
     3901 gtgcagtacg ggcgcccgtc agacgtccgt tgccatcaag agcgataaag gattttgcgt
     3961 acatagtcat attcctcagc cgccagacga cacgcaggca acaaccgtcc cggtacgggc
     4021 agcgtggtca gggtgtgaat ggcggttcag ttaaaatctg gactggagtt tgggtaaagc
     4081 aggctggttg ctgttacagc actgtggttg tcacacaatg gaacactgac gtaaaaaaca
     4141 gtcagtgggc gagtggatac atcgttgccg ccagacaaca acccatactt ttggtttgcc
     4201 ggatattggc gcgccagtcg tgctcaactc cggctacagc caaaattgcg ctcaccggtg
     4261 cagtgagctt tggtatgttc cttcgccaga tagtcagcac gttccagcac ctgctgaaag
     4321 ccagtgtcat catcgcgttc cagccacacc gccggccagg ccggaaaatg caccagcgtg
     4381 gc
//
LOCUS       DAJOWB010000230         4308 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJOWB010000230 DAJOWB010000000
VERSION     DAJOWB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.231"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(95..658)
                     /gene="fimA"
                     /locus_tag="OHO85_004718"
     CDS             complement(95..658)
                     /gene="fimA"
                     /locus_tag="OHO85_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCQ2673957.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            complement(1090..1298)
                     /locus_tag="OHO85_004719"
                     /pseudo
     CDS             complement(1090..1298)
                     /locus_tag="OHO85_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1972..3495
                     /locus_tag="OHO85_004720"
     CDS             1972..3495
                     /locus_tag="OHO85_004720"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCQ2673958.1"
                     /translation="MNDKINQDTINKALWAACDTFRGTISADTYKDFILTMLFLKYIS
                     DVWQDHYDEYKKQYGDAPELIEAMMANERFVLPKSASFYALYERRHEPGNGERIDQAL
                     HAIEEANGTKLKDAGKSVFQDISFNTDRLGEEKQKNTILRQLLEDFAGQDLNLKPSRV
                     GTLDVIGNAYEYLIKNFAASGGQKAGEFYTPPEVSDLIAELLDPQPGDTICDPACGSG
                     SLLMKCGRKIVSGHDSRNYALFGQEAIGSTWSLAKMNMFLHGEDNHKIEWGDTIRNPK
                     LLDKNGDLMLFDIVTANPPFSLDKWGHDEAENDKFGRFRRGVPPKTKGDYAFISHMIE
                     TLKPGTGRMGVVVPHGVLFRGSSEGKIRQKLIDENLLDAVIGLPEKLFYGTGIPAAIL
                     IFKKQKVDDKVLFIDASREFKAGKNQNQLSEENIKKIVKTYRDGDNVEKYAYLASLKE
                     IQDNDYNLNIPRYVDTFEEEDEIDLLAVRAEREQLKAELAKLETEMAGYLKELGYGS"
     gene            3485..4162
                     /locus_tag="OHO85_004721"
                     /pseudo
     CDS             3485..4162
                     /locus_tag="OHO85_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         1283 a          916 c          988 g         1121 t
ORIGIN      
        1 gagatgaata ttggtaaatt gctcgggtgt gcgtagccat gccacacaca ccctgtttca
       61 atttcagatg acgtatagcc accttaagat tgaattatag atattgaact ttgaaggtcg
      121 catcagcgtt tgcagtacca gctacggact gcccgagagc aatataacga gcctggaatg
      181 gcagtatatt cgtgccatca atcaagtcgg ttttagcact aaaagtggcc ccatcaagta
      241 ttaatacctc acccgtacgg tccaaaattt gaataccgac attttgggca gagcctgccg
      301 ctgaactctg cagcgctaac gtatcgtcat tactggtgac agtagtaccc aggaatgcaa
      361 cagctgcatt actggaaaca ttggtatcac aatcattcag cttgatattg aagccaacgg
      421 cggagctgag gtcacccgct ttagccaggc gagaagaacg aacctgaccc agctcaaccg
      481 tttggttcat tgattctgaa tcaattgcac aagcggcatt taccacttca cctttaaaat
      541 gaataacacc gccattaaca gtggtcgtta ctgattcatc accctcggct gcgcttaccg
      601 cagcagacga catcgccaac acagaaacga caatcatacc aacatgtttg agtttcataa
      661 ttctccaaaa tttatattta agtagctgaa taaagagagt taacaattaa gtcattctgt
      721 tattgcttaa aaacaagaat cattcttgat tggttgttgc cctgagaata tagtttccag
      781 ggttattaca tttcacgaag aacgataata tctttaaatc acaaggacac ctaatcacaa
      841 caagtttatt gtgcacttgt tgaagagagt atttgcaaaa acattttatg tcgctaaata
      901 acatgacgat ttcattatgc atgttattta taaaccgtga ctaaacagaa tattattgcg
      961 aacaagggaa gtgtcaggaa aggtcaaggg ctaccaatgg ttaatatggc tataaatcat
     1021 tagctatggt taatgtttgc ttgctgaaac taacaccgaa ttgctctaaa cagataagtg
     1081 cagggtttat taaagctgac cgacagactt tcttaacctg gtaatatctc tcttgggtga
     1141 ctctccaaac atccgcgaat attcccgact gaaatgggat agctttcgta accgacagca
     1201 taggctgctg tggtgacatc gtaatgttca ttcagcatcg gtcgcctgac ttcattgaga
     1261 cgtagccatt tctgataccg gagtggactc attcccgctt gatccgtcct taaataagta
     1321 acagcaccct ctggtgtttt cgtatgcagt gtctatgcga cagacactgc gctcttagca
     1381 tggggcaggg aataaccagg ctatttctcc atgcacaggc caattatgaa agaggagagg
     1441 cttaagttct tgaaccctga acgaataacg actaccagaa aagcacttcg catcactcag
     1501 acatgattta acttatgttg tgcgagagtt cagttcataa accatatggt acatatctca
     1561 ttgctttaaa agatattttt aatagaaaga tgaaatcact tcaagagaac ttcatgaact
     1621 aacaggaaat gcacctctta tgagtatttc ctgacagctt aatctgcttc agttcatgga
     1681 ccattcccaa tgtaataaaa ggttgtaaaa tgcgttttac cgtcactggg gtagggtgta
     1741 ttgatatcac ggggagtagg tcaatccagt accaagccgc gcgttagtgt ttgtttcatc
     1801 gaccagtcca ccacattacg ggcaaaaaga gtcctattcc cctcaggaaa aatccacttc
     1861 gcacgatgcg gctttgacgt gcctgaagac gcaccttaag gtattatctg caccaaattt
     1921 tctacgatga atacaacaca tcgtaaggtt ttcttcatag caggatgatt catgaacgac
     1981 aaaatcaatc aggacactat caacaaagcc ctgtgggccg cgtgcgatac cttccgtggc
     2041 accatcagtg ccgataccta taaagacttt atccttacca tgctgttcct taagtacatt
     2101 tcggatgtct ggcaggatca ttacgatgaa tataaaaaac agtatggtga tgcaccagaa
     2161 ctcattgaag ccatgatggc caacgagcgt tttgtactgc caaaaagtgc cagcttttat
     2221 gcgctgtacg aacgccgcca tgagccgggt aacggtgagc gtatcgatca ggctctgcat
     2281 gcgattgaag aagctaatgg caccaaactg aaagacgccg ggaaaagcgt gttccaggat
     2341 atttcgttta acaccgatcg cctaggtgaa gaaaaacaga agaacaccat cctgcgtcag
     2401 ctgctggaag actttgccgg tcaagatctt aacctgaagc cgagccgtgt cggtacgctg
     2461 gacgttatcg gtaatgccta cgaatatctg atcaaaaact ttgctgccag cggtgggcag
     2521 aaagccgggg aattctatac cccgccggaa gtttcggacc tgattgcaga actgctcgat
     2581 ccgcaaccgg gtgataccat ctgcgatcca gcctgtggtt ccggttcatt attgatgaag
     2641 tgtgggcgta aaattgtctc cgggcatgat agtcgtaact atgcattgtt cggtcaggaa
     2701 gcgatcggtt ctacgtggtc gctggcgaaa atgaatatgt tcctgcacgg ggaagacaac
     2761 cataaaatcg agtggggcga taccattcgt aacccgaagc tgctcgataa aaatggcgac
     2821 ctgatgctgt ttgatatcgt gaccgctaac ccaccgttca gcctcgataa gtggggccat
     2881 gacgaggccg agaacgataa gtttggccgt tttcgtcgcg gtgtaccgcc gaagactaaa
     2941 ggagattacg cttttatctc gcatatgatc gaaaccctga aaccgggaac tggccgtatg
     3001 ggcgtagttg tgccacacgg tgtgcttttc cgtggctcca gcgaaggtaa aattcgccag
     3061 aaactgatcg acgaaaattt gctggatgcg gtaattggcc tgccggaaaa actgttctac
     3121 ggtacgggga ttccggctgc gattctgatt ttcaaaaagc agaaagttga tgacaaggtg
     3181 ctgtttatcg atgccagccg tgaattcaag gcaggtaaaa accagaacca gcttagcgaa
     3241 gaaaacatta agaaaatcgt caagacctac cgtgatggcg ataacgtgga gaaatacgcc
     3301 tatctcgcca gcctgaaaga gattcaggac aacgactata acctcaatat cccgcgctat
     3361 gtcgatacct ttgaagaaga ggatgagatt gacctgctgg cggtgcgagc agaacgcgag
     3421 caactgaagg cggaattggc gaagctggaa acggagatgg ctggatattt gaaggagttg
     3481 ggttatggct cgtgattcgc tttacccagt gagcgttaaa gctggaaaac cgatccttgg
     3541 aatgctacct acaggatggc aaaagttatc attagaaaag tgcttaaata tagaggcaag
     3601 aaaggcatat atacaagata atcaagagta tgacttagtt acagtaaagc gctccagagg
     3661 tggcgtaata agacgtgagc atcttaaagg aaaagatatc tccgtaaaaa gtcaattcta
     3721 tattaaagaa ggcgactttc tcatatcaaa acgacaaatt gtgcacggtg cttgcggtct
     3781 tgtacctaaa gaactttctg gctcaattgt atcgaatgaa tattgtgtgc ttacagggaa
     3841 gtccggtttt tatcttcctt atatggaatt tctatcggag tctctatatt tccagcaaac
     3901 ctgcttccac tctagcattg gtgtgcacat agagaaaatg atttttaagt tagatagttg
     3961 gtttaagtgg ccatttaata tccccccact gtctgaacaa aagagaatcg ttaaaatcct
     4021 gtcaacctgg gataaggcga tttcggtgac ggaaaagctg ctcgccaata gtcagcagca
     4081 gaaaaaagcc ctgatgcagc aattagttac cgggaagaaa cgcttgctgg atgagaatgg
     4141 ggtgaggttt agtggggagt gggaacagtt tccattaaag gttatggccg aggtgattgt
     4201 aagccctgtt gataaaaaaa ctgaggccaa tgaaattcct gtagaactct gcaattatac
     4261 agatgtttat tacaataact atattaccaa taacattaat ttcatgaa
//
LOCUS       DAJOWB010000231         4257 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJOWB010000231 DAJOWB010000000
VERSION     DAJOWB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.232"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(163..676)
                     /locus_tag="OHO85_004722"
                     /pseudo
     CDS             complement(163..676)
                     /locus_tag="OHO85_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
     gene            complement(737..841)
                     /locus_tag="OHO85_004723"
                     /pseudo
     CDS             complement(737..841)
                     /locus_tag="OHO85_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(843..1166)
                     /locus_tag="OHO85_004724"
                     /pseudo
     CDS             complement(843..1166)
                     /locus_tag="OHO85_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1912..2889
                     /locus_tag="OHO85_004725"
     CDS             1912..2889
                     /locus_tag="OHO85_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCQ2673959.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLQPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3174..3914)
                     /locus_tag="OHO85_004726"
     CDS             complement(3174..3914)
                     /locus_tag="OHO85_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2673960.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLNGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALVGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(4035..4223)
                     /locus_tag="OHO85_004727"
     CDS             complement(4035..4223)
                     /locus_tag="OHO85_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HCQ2673961.1"
                     /translation="MESEIFSCLLKASAGFFLRFCLSWHYPNSPKNGKPALSPHHDWL
                     KTGSQRQNAPLCIWFYPQ"
BASE COUNT         1031 a         1087 c         1040 g         1099 t
ORIGIN      
        1 ctttttcggt tgaaaagaaa ttgattgaat tactggaaaa acaatctggt ctccatacgt
       61 ctgtggattg cagtactccc cttacagagc agagtctgtc gagaaatgac ggtgaagacc
      121 agaccagaag aaacgctgaa aatatgttcg gagatgactg aactaaccca gcctggccag
      181 gggaatatct cgccatttag ttgtataaca ctgactcaac atttcccgtt tcatttgcca
      241 ttcaggggcg gttcctcgcc ccgcaaacca cactttccct tttcccgact ggttcagttc
      301 atcgagtgtt ttcattaact tttcactgtt tttacggggc tggatttcat caaataatcc
      361 cggctgcgct atacccgatg gtgtgaaatc agccagcatg actcctgcct tcatatagcg
      421 gtacccttca cgccagacat ggtttaaggc tctgcatgcg gcggcaataa tgtcccggct
      481 gtcctgtgtg ggcaatgaaa gcttttccac agcggcattg ctgtaacagg gttcaggggg
      541 atgtccgtac aaatgtcgtc acctgccggc aatactgacg ctccccacgt agtttctctg
      601 cggcccgctc tgcatactga acaacagcct ggtgcatggc atctttgtct gtgattcgtt
      661 caccaaaact gcgactatgt caacgccacg atgtttgacc gttatttgcc attttcatgc
      721 ccgctaaata ctttcgttac tgatgctgtc cgctttgcac catttcatca tcactgattg
      781 gcgttgtgtt tttggtcagc actccagctt tacgtttctc ttttaaccgg taactctctc
      841 cttgttcttt ttgacggacg cattttacgt ttgggctgga tgtagtaacg cagcatggaa
      901 cgtccgcccg tataacccat tgccttaatc tccgcgaaga taacctcgct attccagaca
      961 ttctctgcca ggcgcatgtc gatgtaatcc ataaacggtt tcagcttaac cattttgtgg
     1021 cgggtctttc tggctggcgg ttcagggtat ttgaggtagc gtctgaccgt ccgttcagag
     1081 caaccaatct gagccgcaat atcgacaatg tacgcgccct gctggcgcat ttgctttatc
     1141 atgtaaaagt cctctctgct cagcatgttg atgtcctttc tggtgtgaga accttaagga
     1201 aacaacatgt tgggtggagc ggacaatcca aatggtgaat taccgtctta tatcactggc
     1261 gctgacacct gtagtttgta tacacaatcc ctggcattga aacacacata tgaagtgtac
     1321 ttcggatatg accgtacgct caggaagtac ggcagattcc gtcaaaaatt acctgtaaag
     1381 acagacatca gaaaaaacag tgctcgccgc agaccggtga ccgggcgaag cccgcgaacc
     1441 ggcgaggaag cgcaatgtat ctgtagttac agggagcagg tacgccctga agccctttca
     1501 taaaacggtt gtgtgcgctg tccttctgga atgatttatc cacatatccg gaggcccgat
     1561 ccagtaaaca gatccatgaa tgatcaacaa aggatccatt aaagatcccc atatcgctgc
     1621 aaaccttgtc actcatgggc cgggaccacg atcacataag ctgtggcatg ttactgataa
     1681 actgtaacat gctaatgata agctgtattc agtaatccat atactgaagt aagttaatga
     1741 cataaactat ggtcagtatg ccagactcag ttgttaaata caggctgcag gtttttcttc
     1801 agtcagttag cggagttctg acacacgatt ttctgtttat tcttttactg tccacaggct
     1861 ggaggctttc tggaaaacga aaattcagac atcaaaaaac tgttcggcga ggtggataag
     1921 tcctccggtg agctggtgac actgacacca aacaataaca acaccgtaca acctgtggcg
     1981 ctgatgcgtc tgggcgtttt tgtaccgacc cttaaatcac tgaagaacag taaaaaaaat
     2041 acactgtcac gtactgatgc cacggaagag ctgacacgtc tttccctggc ccgtgctgag
     2101 ggattcgata aggttgagat caccggcccc cgcctggata tggataatga tttcaagacc
     2161 tgggtgggga tcattcattc ctttgcccgc cataacgtga ttggtgacaa agttgaactg
     2221 ccttttgttg agtttgcaaa actgtgtggt ataccttcaa gccagtcatc ccgcaggctg
     2281 cgtgagcgca tcagcccttc cctgaagcgc attgccggta ccgtgatttc gttttcccgt
     2341 accgatgaga agcacacccg ggaatatatc acccatctgg tacagtcagc ttactacgat
     2401 actgagcggg atattgtgca gttacaggcc gatccccgcc tgtttgaact gtaccagttt
     2461 gacagaaaag tccttctcca gcttaaggcg attaatgccc tgaagcgacg ggagtccgcc
     2521 caggcactct acacctttat agagagcctg ccccgggatc cggcaccgat atcgctggcg
     2581 cggctgcgtg cacgcctcaa tctgaagtct cctgtatttt cccagaacca gacggtcaga
     2641 cgggcaatgg agcagttgcg cgagattgga tatcttgatt acacggagat ccagcggggg
     2701 cggacaaaat tcttctgtat tcactaccgg cgtccccggt taaaagcgcc gaatgacgag
     2761 agtaaggaaa atccgttgca accttcacct gcggaaaaag tcagtccgga gatggcggag
     2821 aagcttgccc tgcttgagaa actgggcatc acgctggatg acctggaaaa actcttcaaa
     2881 tcccgctgaa cataaactgt agtcagtgaa gagcgctcct ttactgacta cagcttatat
     2941 taaccggtgc agtgagtggt ctgctcactg cagtttatat tctgtttcct gcagtgctgt
     3001 ctgtagctga gctgccatct gcctgtccct tacgtaagtg atcccgtaac ctgatcctga
     3061 ggcattgctc ccttcataaa acatgactta ctcactacag cttatataca tgctccagct
     3121 tatgttatgt ctgttctgct gaccacagct tatataagga agcgcgtatg ccgtcacctc
     3181 aggggaggca gtgtacgcag gatctccgca gcatcccgcc cgtcacctgt gaaaggcact
     3241 gccagcgtgg cagccatatc cagtgcaaac actctggtat aaacctccat cgaacgtgga
     3301 tccctgtgac caaccagtgc ctggatgact ttccggggct ggcggtgata gagcatgtgc
     3361 atgatatagc tgtgccggaa ggtgtgtggt gtgaccggaa tcgaaaagtg tactccgtca
     3421 gcttcggccc gtctgacagc ctgcttcagc cagttgcgca tggtttcgtc ggtcacggcc
     3481 cataatggtt cacgacgacg gggccgggtg gtgatcatcc agctttccat ctgcctgaca
     3541 tagcttatat ctgtcagcgg aaccaggcgc acttcatctt ttggcgggcg tccgcgtcgc
     3601 gcacgcactt tttcggacag gatccgcaca aacggtctta ctccattcag gtcaaatgat
     3661 tccggcgtca gcatccgggc ttcgccaata cgcattccgg tattccagag ggtggcgaac
     3721 agcatatggt gacgctgatc cggcatatag aagagaaggg cactcacttc cggggccagc
     3781 aggtaggccg gtgtggcccc cgtggaagtg gccattcttc tcagggagag cgctgttgca
     3841 aaatccaccc ccggcgcaat gggtaacagg gagacgcgtt caggtgaatt ctgcagggga
     3901 atgacattgt tcatgagtta tttttcacgt agcacacgca ctgtttatgg atacaggtat
     3961 tctgacctga acgcccggat ccttccacga caataaatcg cagcgcgcgg atccttttgt
     4021 ttgattatca cgcattattg cgggtaaaac caaatacata aaggcgcatt ttgccgctgg
     4081 gatcccgtct taagccagtc atggtgcggg ctgagggctg gtttaccgtt tttgggagaa
     4141 ttggggtaat gccaggacag gcagaaacgg agaaaaaatc ctgcagacgc ctttaaaaga
     4201 caactgaaaa tttctgactc catattcctc tggtttcaga ctgcttaatc acatcat
//
LOCUS       DAJOWB010000232         4232 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJOWB010000232 DAJOWB010000000
VERSION     DAJOWB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.233"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(234..1301)
                     /locus_tag="OHO85_004728"
     CDS             complement(234..1301)
                     /locus_tag="OHO85_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015874993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HCQ2673962.1"
                     /translation="MSGGIFEGVEKNLIGGLTNVAQGQMMIYSNMMMGLAVMSATIYI
                     IWRGYQTLAGKLSTPVEDTMWDIMRMAIILSFVANLGGYLDGVIDAINGLKEGFSGGD
                     NIWRMLDSLWQNAKFLGKTLHDLDDSEFIKDEGTIAELFVWGGTFALMIVTAFVSMVA
                     EITIMLLSVTAPIFIFCLMYGFLRPMFNNWLQNIFAAILTILFSALSLRIVIIYTDSV
                     MTTAIDGAARANIVQLGAQVCLSGICAAILVYISARIASALAGASATATMQGAAAIGI
                     GAAAYGVGKMAGGAKDKLEKNIDGWNAHDDGAKRAPKGGALGYNAAKARKYAIEQMIA
                     RNEAREIARSREEAIKKYGAK"
     gene            complement(1303..1581)
                     /locus_tag="OHO85_004729"
     CDS             complement(1303..1581)
                     /locus_tag="OHO85_004729"
                     /inference="COORDINATES: protein motif:HMM:NF033894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HCQ2673963.1"
                     /translation="MKKFATIASALIICFSITGCDNKTSVDWYMKHHEDMFSKYTECL
                     LADSWHEVSCQNARSAMNLEKEKPDVQDGLAAAREKLIERNRNLMGHK"
     gene            complement(1641..1865)
                     /locus_tag="OHO85_004730"
     CDS             complement(1641..1865)
                     /locus_tag="OHO85_004730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673964.1"
                     /translation="MADMKTTTQTRVIDLEILEEVITRAEFAHSLAGLITESANFKKL
                     SEHQQNALMALMTFTYDVKNAISELMNSAE"
     gene            complement(1945..2913)
                     /locus_tag="OHO85_004731"
     CDS             complement(1945..2913)
                     /locus_tag="OHO85_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCQ2673965.1"
                     /translation="MSEDKFLSEYSPKDSVWDSHRAFTDSVGGIYRSAAEFERYAERM
                     ASCSGLLRFGWSTLMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     AQFPSSRWLFLTLTVRNCEIGILGDTLTLMNAAFKRMEKRKELAPLQGWIRATEVTRG
                     KDGSAHPHFHCLLMVPPSWFSGVAYVKQARWVELWRDCLRVNYEPNVDIRTVKMKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPDWFLELTRQLHKRRFISTGGALKNILQL
                     DRETNEDLVIADEMGDGTDDGKRTAFVWDSGRRRYRRAPEKDRFDN"
BASE COUNT         1169 a         1075 c          877 g         1111 t
ORIGIN      
        1 aaaaaaaata actcaaggag gcttttaaat gatgtttgtt tatttattca agagcattgc
       61 aatctggatt gatataaata aaacgataac cataatcagc gttctttttc gtaatgagta
      121 attattaaaa acagggaaca ttttgctcac aacaacatac tgaataatta aaacgctacc
      181 aaatgcaatg attaaaaata tagcaaatga gaatatatgg attgtaattt ccattattta
      241 gctccgtatt tttttatagc ctcttcccta gaccgagcaa tttctcgcgc ttcgttccta
      301 gcaatcatct gctcaatcgc gtatttacgt gccttggcgg cgttataacc taatgctcca
      361 cctttgggcg ctcttttggc accatcatca tgggcgttcc agccatcaat gtttttttcc
      421 aatttgtctt tggcaccgcc agccattttt ccaacaccgt atgcagcagc gccaataccg
      481 atagccgccg ccccctgcat agtggctgta gcactcgctc ctgccagtgc agaagcaatt
      541 cttgcagaga tatacaccaa tattgccgca caaataccag acaagcatac ctgggcacca
      601 agttgaacaa tattcgccct cgcagcaccg tctatagcgg ttgtcattac tgaatctgta
      661 taaataataa caatcctcag agaaagcgca gagaacaaga tagttagtat tgctgcgaaa
      721 atgttctgta accagttgtt aaacataggg cgaaggaaac catacatcag gcaaaagatg
      781 aatatgggcg cagtgacact gagtaacata atcgtgattt cagcaaccat tgatacaaaa
      841 gccgttacaa tcatcagcgc aaaagtacca ccccatacaa acaattcagc aattgtgccc
      901 tcatctttaa taaattctga atcatccaaa tcatgtaatg tttttcccaa gaattttgcg
      961 ttttgccaga gactatctaa catccgccaa atgttatcac ctccagaaaa gccctctttc
     1021 agtccgttaa tggcatcaat cacgccatca agataaccgc ccaaattagc cacaaaggac
     1081 aggatgatcg ccatcctcat aatgtcccac attgtgtctt ccactggcgt ggagagcttg
     1141 ccagctaagg tctggtatcc tcgccatatg atgtagattg tagctgacat taccgccagc
     1201 cccatcatca tattggagta gatcatcatt tgcccttgag ctacattagt cagcccaccg
     1261 ataaggttct tttcaactcc ttcaaaaata cctccgctca tatcatttgt gccccatcag
     1321 attacgatta cgttcaatga gcttctcgcg tgcagcagca agcccatcct gtacatccgg
     1381 tttttccttc tcaagattca ttgcacttct agcgttctgg caactaactt catgccatga
     1441 gtcagcaaga agacattcgg tatatttaga aaacatgtcc tcatggtgtt tcatatacca
     1501 gtcaacagaa gttttattgt cacatccagt tattgaaaaa caaataatca aagcagaagc
     1561 gatagtggca aattttttca tgattaacat tcctgtgtta attattgaga ctcaatgata
     1621 ttgagtctca tatattaaga tcactcggct gagttcatta attcagatat agcatttttg
     1681 acatcatacg taaaagtcat taatgccatt agcgcgttct gttgatgctc tgaaagtttt
     1741 ttaaaattag cggattcagt aattaatcca gccagactat gcgcaaattc tgcgcgagtg
     1801 atgacctcct caaggatctc gagatcgatt acacgggtct gtgtggtagt cttcatgtca
     1861 gccattacag cctcctaatc aggttgtgtg gttagcggtc tgtcggaagg tgagagcttc
     1921 ggcaggccgc gcaaaatccg atactcagtt atcgaatcta tctttctccg gggcgcgcct
     1981 gtaacggcgc ctcccggaat cccaaacaaa cgcagtacgt ttgccatcat ctgtgccatc
     2041 ccccatttca tcggcaatga ccagatcctc attggtttcc ctgtcaagct gcaaaatgtt
     2101 cttaagtgcc ccacctgtcg aaatgaaacg acgcttatga agctgccgcg tcaattcaag
     2161 aaaccaatca ggatcgttag ccatatcttc cggtttaaca gaatatttca gggtttcagc
     2221 aacggcgctc tgtagctgtt ccgcaacgtt ggccactact tcgcctgttt tcattttcac
     2281 agtcctaata tcaacgttcg gttcgtaatt cacccgtaga caatctctcc atagctccac
     2341 ccaacgagct tgtttgacat aagcaacacc agagaaccat gatggaggca ccatcagcaa
     2401 acagtgaaag tgcggatgtg cactaccatc ttttccccga gttacttcag tcgctctaat
     2461 ccatccttgg agaggagcaa gttctttacg cttctccata cgtttaaatg cggcattcat
     2521 tagtgtcaga gtatccccta aaatccctat ttcacagttc ctgacagtta acgtcaggaa
     2581 caaccagcgc gaactgggga attgagcaac aatttttgga agagcctggt aaaaacgggc
     2641 ttgccacatg agcgttcttc tccactggca gacaggacaa tgacggacac gacagaattg
     2701 cgcactacga agccttaagc gcgtctctcc agtctccatg agcgtagacc aaccaaaacg
     2761 caataaaccg ctacaggagg ccatacgctc tgcataacgc tcaaattcag cagcacttcg
     2821 gtaaatacca ccgacagaat cagtaaacgc acgatgtgag tcccaaaccg aatctttcgg
     2881 gctgtactcc gacaggaatt tatcttctga catgccacct gctccgatct gtgcaggtgg
     2941 tgatttgatg tgttatattc atcacgtacc tgtgcattgc gtgccagtta ctgtcaccgt
     3001 ctgacggcta atcttccggt gacactttca aatgctgagg agtgcgctaa cactcctcag
     3061 cgccgcttaa aaacacttca attttgcgcc aaatcctttg atccgtcaaa caccactcta
     3121 agccgcgtca atactggctt gaagcggtat tttcaacccg ccacttatcc cttaagtata
     3181 aatacaagga tcgcgcgcgc gagcaacatc gcaaaaacgc tcattcttta atcgtttttg
     3241 gataacctaa tcgcttcaac atcgacggtc tacgaccacc taccaaccga gtagctgcta
     3301 gtcctgtgca attttggctt agcagggggc taacgcggtc cctgacgaca tgaaagccct
     3361 tggttagggg tatcggcgcg gcctggcatt tgtgattctc tgaaaccgtt tttcgcgcct
     3421 tacatgccgc tgacgcggca tcttcccgcc atcgggcatg acttaactaa cagccaacat
     3481 tagagagagc gatccgctcg ccgcaaccct agcgaccgaa cgttagtgag cgagtgggtg
     3541 aggaagcgga agataactgt aacggacata atgcactcac gcatcacagc ataagaagaa
     3601 taaaagtatt acagcaaata tattcttgct gaattatgcc gatatttcag cgcgggtttt
     3661 agcttctgaa acatccgccc aagtggattt tcagaagctg gggggagccg ccgaaaattt
     3721 ccgccccagc gagaaatagg cacgccaacg tgccggtttc ggtggggggc ttgccccgcc
     3781 acaccgtcaa gccaggtaat atttcccgcc ccagcggggg atattaccga ttaggcgcga
     3841 cccccgtctt taaagatgaa ttcccatttt ttcaagttta cgcagaaaaa ataggctaaa
     3901 ttcatcgtct taaagacggg ggtcgcgcgt agcgcgcgac ggtgtggccg cttttgacct
     3961 tcggggtttt ggggcggcgt ccagccgaag caaaaccccg tgtggcgcga agttcgggcg
     4021 ttcagccgga acgaagcacc acgggttttc atgccgttaa tggggtacag aaatggggtg
     4081 cactgatggg gtgcatagcg tgctctattg ctgcacccca tcgttgtacc ccatcaacac
     4141 aaacaaacat cgatgggagc atattatggg aacggggcta tgctcttata agctactccc
     4201 atatactgct catataaata aaaaagcctc cg
//
LOCUS       DAJOWB010000233         4232 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJOWB010000233 DAJOWB010000000
VERSION     DAJOWB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.234"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            505..681
                     /locus_tag="OHO85_004732"
     CDS             505..681
                     /locus_tag="OHO85_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673966.1"
                     /translation="MNLVDAFVKKVISGPYKEYGKWWIDVEYISWGVPGKTRLMFESK
                     EQALEVKEGYKFLT"
     gene            complement(746..1042)
                     /locus_tag="OHO85_004733"
     CDS             complement(746..1042)
                     /locus_tag="OHO85_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673967.1"
                     /translation="MSALRHVLSGILFSGKNRLQVLAKTSGGAGFGALVGEELSITDT
                     IETVVTGSVRANNGVLSPVMEKRMINLLIIVLRAVVAVANALIAVLELIRELID"
     gene            1453..1593
                     /locus_tag="OHO85_004734"
     CDS             1453..1593
                     /locus_tag="OHO85_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673968.1"
                     /translation="MLAMTAFTLMTRRGICEMQNVAPDGKGIRLELIGFRGNETIPDT
                     LH"
     gene            complement(1665..1817)
                     /locus_tag="OHO85_004735"
     CDS             complement(1665..1817)
                     /locus_tag="OHO85_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCQ2673969.1"
                     /translation="MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATL
                     AYEVKR"
     gene            2109..3317
                     /gene="trbA"
                     /locus_tag="OHO85_004736"
     CDS             2109..3317
                     /gene="trbA"
                     /locus_tag="OHO85_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HCQ2673970.1"
                     /translation="MSYNRQPVAEDPMQIWGAVGVLLILLLFVIWLFLPEVVYASCLI
                     LHTLWGLVDWGPFHNYAAPRYNLLAMTGNNAANISYSQWVNVMEQTIGILWMYLLPVT
                     LWCLWEWYQHPGQSRFTRRPVDITRLPYIFASLSPAIAPVLADGDPEKLFHGGKRPER
                     RVALTPEAFVEQHTLITNMQLDVAAARRCFMAQLGKPLTSWKDMAPHEKALFAIFGLQ
                     YFLDDRKAALKLMDTLNLSCRIKSKRDSGKFCTPVYSLAKSAFQRVIKSNGAQQWLKQ
                     HRYVRSGLVWLYAHDLRLTPPNWIWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     EAEAMRFGLPCPEPCVDEAVEGLRRDMISLGLIWDEPQPDRDRKRRILTNWSLTDDIL
                     PRTPATDNEF"
     gene            3336..>4232
                     /gene="trbB"
                     /locus_tag="OHO85_004737"
     CDS             3336..>4232
                     /gene="trbB"
                     /locus_tag="OHO85_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbB"
                     /protein_id="HCQ2673971.1"
                     /translation="MTIEYFATRIAKHISVQAIWPDGRTEIIAVLPRDALSGLVTRNN
                     QLIHIAFDAMGTRNRETVLIDKQEHNADQILTAIDSCLRLEYLMERRFSRTPLFRGII
                     ASVVLFVMATIGFSLFRYVDRVFWDDTTPEAVQTAGEPRLLPPHLNHTVPLNEGIQLP
                     VPPKKDVQVPEKTITSKNPEAAAARHNLATVLKRNADRGMFTINLSSGHERTLFAFLD
                     PACPNCRLLEPALKRLASDFNVVIYPVSVIGGEESTDRVAPLLCEKDAQKRAAGWHRL
                     YSADNGMMTPSEETTPADETCLK"
BASE COUNT         1182 a         1081 c          946 g         1023 t
ORIGIN      
        1 gcctgacacc ggccttcacc taatgcatag atcgcagcaa gtgtaacgtc ttttgattta
       61 ctcttcatca tttcaaacaa gaaagtatta gctatcgcag gaggcatgct cataatcaat
      121 cctcttcatg aaataaacaa tggcactcac ttacaaacag gagtccacta tcgcaagcga
      181 ctcaacttag caatatctct caatttgaat gaaaaaacca gatggcatgt gacagggaac
      241 cgtccgcatg atgtccgtga ccatttaacc ccataagcta accttgtcac aaacgggcgc
      301 agaacagcct gccagacaga caacaaaaca aaagtccaag ataaaacatg aataccggta
      361 tctcaatgcg aagaaataat ctccgtagaa acccccatga gaaaaatatc atatgggaga
      421 aaatcgcaat tatcacagcc agcgcattca ttattttcgc tgtgctctgg cattaattat
      481 tttcaacaca aaaaggaaaa aacaatgaat ctggtagatg cctttgtcaa aaaagtaata
      541 tccgggccat ataaagaata tggaaaatgg tggatagatg ttgaatacat ttcatggggc
      601 gttcccggga aaacaagact gatgtttgag tcaaaggagc aggcactgga agttaaagaa
      661 gggtacaaat ttttaacgtg agatctcttt ttgttaaagg gcaggaagcg gtaactccct
      721 gcccttagtt acgtttccag gcttatcagt cgataagttc ccggatcagt tccagaacag
      781 caatcagcgc gtttgcaacg gcaactactg ccctgagaac gatgattaac agattaatca
      841 tccgcttctc catcactgga gaaagaaccc cgttgttcgc tcttacactg cctgtcacaa
      901 cggtttctat agtgtcagtt atagacagtt cttctccgac cagagcacca aaaccagcac
      961 cacctgatgt tttggccagg acttgtaacc tgttcttccc gctgaacaat atgccggaca
     1021 gaacatgcct cagagctgac atgccgagta tacacttatc cagtacttgc agaaatataa
     1081 ttccagacgt actatctaca ggtcagttat agacagttct tttccgacca gagccaccca
     1141 ctctgtaacg gaacagtaaa aaaaccgacc tcaccagtcg gtttttttac atctgttgtt
     1201 tctcaaaatc cgcccgacat gcccgtcaca ccggggaaac tcatgctttg aatcagaacc
     1261 aaccatctca actttgccgt ctcctgaaaa aactggcaca ttcagttcga taactaccca
     1321 taaatacaga gagaaaatcc gatgtcagaa aagtggagta aagaattcga ctcttgcttt
     1381 caggtgggga gcatagaata aattgccgat gctctcgaga aacgcagcgg aagcgaggaa
     1441 aatgcgttag ccatgctggc aatgacagcc ttcacactca tgacccgacg tggtatctgc
     1501 gaaatgcaga acgtggcccc ggatggcaaa ggcattcggc tggagctaat cggcttcagg
     1561 ggaaatgaaa caataccgga tacgcttcat tgaagaagaa agggcgcaat gaggtcagcc
     1621 ttcgcaacaa agcggcggca gaaaacctgc cgccgtgttg cccgttaacg tttaacttcg
     1681 taggctaacg ttgccacaag cactgtgttt ccctgctgga gccggagtcc gcaaagcgaa
     1741 tccctcacca tccagacaaa acacagaatc gtcacacaga tgacgattaa catcattaaa
     1801 aacgttcgct gtggcatcat agcccctcca ttgccttacg gcggttatcg ggctactata
     1861 atgttgtcta ggcattacag tagagccctt ggttgattga taaaaaaatc tccaggggct
     1921 ttctgctgta taccgtccac aaaacgcttt cggcatacag cctcaagcgc ccgcagctat
     1981 tctaacgact ttcgtatctg caacaatcct tttatcacac ggctaattcc tctggttagc
     2041 tcttatcgcc tgcaaataaa tttttaacct ttccgtttga tttaaattcc agaaatggag
     2101 actttgtcat gtcttacaat cgacagcctg tcgcagaaga tcctatgcag atatggggcg
     2161 ctgttggtgt ccttcttatc ctgctgctat tcgttatatg gttattcttg ccggaagtgg
     2221 tttacgccag ctgcctgatt cttcatacct tatggggact ggttgactgg ggaccatttc
     2281 ataactatgc agcaccacga tacaacctgc ttgccatgac cggtaacaac gccgccaaca
     2341 taagctatag ccagtgggtt aacgtcatgg agcagaccat aggtattctg tggatgtacc
     2401 tcttacctgt gacactctgg tgtttgtggg agtggtatca acatccggga caaagtcgtt
     2461 tcacccgcag accagtagat attacccgcc tgccatacat ctttgcttcg ttgtcccctg
     2521 ctatcgcccc tgtactggca gatggtgatc cggaaaaact ctttcatggt ggcaaacgac
     2581 ctgaacggcg ggttgctctc accccggaag catttgtcga gcagcatact ctcatcacca
     2641 atatgcagct cgatgttgca gctgcaagac gatgtttcat ggcccagttg ggtaaacctt
     2701 taacgtcctg gaaagacatg gcaccacatg aaaaagcgct ctttgccata tttggtcttc
     2761 agtatttcct ggatgatcgt aaggcagcat taaagctcat ggatacactg aacctctcat
     2821 gcagaattaa aagcaaacgg gacagtggga aattctgtac ccctgtttat tcactggcca
     2881 aatcagcttt ccagagagtg attaaaagca acggagcaca gcaatggctg aaacaacatc
     2941 gctacgtccg cagtgggctg gtctggctgt atgcacatga cctgcggctt acgccaccca
     3001 actggatttg gctgaaaggc gtggacagga cgctgtttta tgcattacac cgcgccaata
     3061 ccacgaaagg atttattgaa ggcgctggcg tggtggccgt tgcacgtgca gaggccgaag
     3121 ccatgcgttt tggccttccc tgccctgaac cctgtgttga tgaagcggtg gaaggcttac
     3181 ggcgggatat gatcagtctc ggtcttatct gggatgaacc acaaccagat cgcgaccgga
     3241 aacgccgcat cctgaccaac tggtccctca ccgacgacat cctgccccgc acacctgcta
     3301 cagacaacga attctgatta tccggagaac tgaaaatgac aatcgagtac tttgcaacac
     3361 gtattgcaaa acatatttcc gtgcaggcaa tctggccgga tggcaggaca gaaattattg
     3421 ccgtccttcc acgggatgcc ctgtcagggc tggtaacacg aaataatcaa ctgattcata
     3481 tagcctttga cgccatgggc acccgtaaca gagaaaccgt cctgattgat aaacaggagc
     3541 ataacgctga ccagatactt acagctattg attcctgcct gcgccttgaa tatctgatgg
     3601 aacgccgctt cagcagaaca cctttgttcc gtggcataat cgccagcgtt gtgctgttcg
     3661 taatggcaac gattggcttc tctctgttcc gctatgttga tcgtgtattc tgggatgata
     3721 caacgcctga agcagtacag acagcaggtg agccacgact gcttccccct cacctgaatc
     3781 acactgtgcc attaaatgaa ggcattcaac tcccggttcc cccgaaaaaa gacgtccagg
     3841 taccggagaa aacaatcaca agcaaaaatc cggaagccgc agctgcgcga cataatctgg
     3901 ctactgttct gaagcggaac gccgatcggg gcatgtttac catcaacctg tcatccggtc
     3961 atgaacgaac cctgttcgca tttctcgatc ctgcctgtcc taactgcaga cttctggagc
     4021 cggccttaaa acgcctggcc agcgacttca atgtcgttat ctacccggtt tcagttattg
     4081 gtggagagga atccaccgac cgtgtggcac cactgctgtg tgaaaaagat gctcagaaac
     4141 gtgctgccgg ctggcaccga ctgtattccg cagataacgg aatgatgacg ccatcagaag
     4201 aaacaacgcc tgcagatgaa acatgcctga aa
//
LOCUS       DAJOWB010000234         4134 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJOWB010000234 DAJOWB010000000
VERSION     DAJOWB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.235"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..280
                     /locus_tag="OHO85_004738"
     CDS             <1..280
                     /locus_tag="OHO85_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673972.1"
                     /translation="EITQPEPAPAQPDETVSEADAEANKAIEYLNNVMDMQSTDMAEI
                     RNARGNVREAIAALQAAGRFEENEELVNGAARHLADLLVAIQKAGVAA"
     gene            277..642
                     /locus_tag="OHO85_004739"
     CDS             277..642
                     /locus_tag="OHO85_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ddrA"
                     /protein_id="HCQ2673973.1"
                     /translation="MTLSAIELMDLSDKLDALMSKAATASGMELLDISDEIDQIMQQM
                     GYGVSGGSSGEEKQPAVHDGVPKLVADFLADKFVDQSTDAFIGTLQDLSQYVGTYIDL
                     DQVKQHTAAWIAANIKEAA"
     gene            655..3642
                     /locus_tag="OHO85_004740"
     CDS             655..3642
                     /locus_tag="OHO85_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673974.1"
                     /translation="MSLSDQVVMATSIETLIELLKNLPDYGRVSYVVTAKGDEVKTAF
                     DIVDASALLVSNTLDGKINPDYPQELQPRDRTRASSLLQVNQISKDLRPAQLTDSGLS
                     SHGAPIIGEDNAVESGNGRTMGIIKAYQDGNADRYREYLIDHATEFGIRPEKVESMTA
                     PVLVRRRLTKVDRVQFAKDSNISDLQEMAASEKAFVDADSITPAMMALFNPSESGDLL
                     SRSNDAFIRGFMTQVGATQAAGLVTEDGRPTRQLVDRIQNAIFAKAYKDARLVRMVAE
                     EPDPDMRNVLTALNAAANDFVQMQALSGEAHKQAVTTIVDSIETADSLDKKALAALKD
                     AVDLVRQSKESGQHITDVIAQGDMFSETAPEVKALALFIVANNRSAKRMATAFKLMAQ
                     RINDELQHQGQALGDMFGGGDVSLQDILRQVSQELENEGMQGISGGLFESASGGSYNG
                     VAPYTSLLLHRASGIKDIIHLIRLLSRTDPQDEQLVQVLAHFVRMPVADVKKWCRLFG
                     ISNSLLRGLLNHASSLGRDGFDEIAQAIKNGDMPPAIDWFSIRPTRVKAFLSAAHSAS
                     PLAEMVQRLSLIFTDHTALGDLTLDEMKEASIQWADQQNEVNSDFLPAFRKAVSKVDD
                     ARGILKAFKALQSRVNKHVGDIDGVTAEGRDILKEYGITPEFIDEIRTDMQREVVSSL
                     QIVARALADANPKSAAIVNRVIGDIEASEGMGALKLFLSRAFNPNGNILPGIIGEAKR
                     YVSEEELEQLDQLLKRFSYNPQTRWQMNQRRMGSVHEKVLSAMNSAIANSSVSEEKAL
                     EWADSFITEEVEEARAGQNGGIDLRKELADIYRLTGGKISTLSKVVHHQGRAYANLNG
                     VVAVNLNDENASALWHELGHHLEYSNPGLLEKARSFLKANVEGDKPSFVNIGGRGKPE
                     WCFRSRLSNIYMAKVYPPASVSNTGKIRQKSPTISKTSATEVFSMALQLYHDKEAAAA
                     SLMNGDGLLELLLGVAKELNNAD"
     gene            3632..3943
                     /locus_tag="OHO85_004741"
     CDS             3632..3943
                     /locus_tag="OHO85_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673975.1"
                     /translation="MQIKIAAPLGGDAIIEFDDNEEVSGRLSIISGDITEDMIAEAIA
                     GANPNSYMGFVNTLDAPASDVLRTLHLYAGWFVDWPAVDGGDDDDDDDDFGDHVDQIV
                     Y"
BASE COUNT         1104 a          893 c         1125 g         1012 t
ORIGIN      
        1 tgagattact cagccagaac ctgccccggc acagcctgat gaaacggtta gcgaagcaga
       61 tgcagaggct aataaagcca ttgaatatct caataacgtg atggatatgc aaagcactga
      121 catggcggag atccgtaacg cccggggtaa tgtccgggaa gcgattgcag cccttcaggc
      181 tgccgggcgt tttgaggaaa acgaagagct ggttaatggc gctgctcgcc acctggctga
      241 tctgctggta gcaatccaga aagcgggggt agcggcatga cactatcagc tattgagtta
      301 atggatctca gcgataagtt ggatgctctg atgtccaaag cggctaccgc tagtggcatg
      361 gagttgctgg atatcagcga tgaaattgac cagatcatgc aacagatggg gtacggtgtg
      421 tccggcggca gtagtggcga ggaaaaacaa cctgcggtac atgatggtgt gccaaaactg
      481 gttgctgatt tcctggctga taaattcgtc gatcagagca ccgatgcatt tatcggtacc
      541 ttgcaggatt tgagtcaata tgttggcaca tacatcgacc tggaccaggt taaacagcac
      601 acggcggcat ggatagccgc caacattaaa gaggcagcat aaggcgtaac agggatgagc
      661 ttaagcgatc aggtggtaat ggccaccagc atagaaacgc tgatcgagct gctaaagaac
      721 ctgcccgatt atgggcgggt ttcgtatgtg gtgacagcga agggagacga ggtaaaaaca
      781 gcgtttgata tcgtcgatgc ctcagctctt ttggtatcca atactctgga tgggaaaatt
      841 aatcctgact atccccagga acttcagccg cgcgaccgga cccgcgcatc cagccttctt
      901 caggttaacc agatatccaa agatttgcgg cctgctcagc ttactgattc cggtttatcc
      961 agccatggcg cgccgataat tggtgaggat aatgccgttg agtcaggtaa tggacggacc
     1021 atggggatca tcaaagccta tcaggacggc aatgcggatc ggtatcgtga gtacctgatt
     1081 gatcatgcga ccgaattcgg catacgacct gaaaaggttg aatcaatgac ggctccggta
     1141 ctggtgcgcc gccggttaac taaggttgac cgcgttcagt ttgccaagga ctcaaatatt
     1201 tctgatcttc aggaaatggc agccagtgaa aaggcttttg ttgatgccga cagcataaca
     1261 ccggcgatga tggcgctttt taacccgtca gaaagcggag atctgcttag ccgcagtaat
     1321 gacgcgttta ttcgcggatt tatgacgcaa gttggtgcca cacaggcggc tggacttgta
     1381 actgaagatg ggcgaccaac acggcaactt gttgaccgga tacaaaacgc gatctttgcc
     1441 aaggcatata aggatgcgcg cttggtaagg atggttgcag aagaacctga tccggatatg
     1501 cgtaatgttc tgacggcgct taatgcggcg gccaatgatt ttgtccagat gcaggcttta
     1561 tcaggagaag cgcacaagca ggctgtgaca actatcgttg atagcattga aacagcggat
     1621 agcctcgata aaaaggcgct ggctgcattg aaagatgcgg tagacctggt aagacaatcg
     1681 aaggagtcag gccaacatat taccgatgtt attgctcagg gggatatgtt cagcgaaacg
     1741 gccccggaag tgaaagcact cgcgttgttt atcgtcgcga ataaccgtag cgcgaagcgt
     1801 atggccaccg cctttaagtt gatggcgcaa cgtatcaatg atgagttaca gcaccagggc
     1861 caggcgctcg gggatatgtt tggcggcggt gatgtgtcgt tacaggatat ccttcgccag
     1921 gtatctcagg aactggaaaa cgaaggtatg caagggatat ccggcggtct tttcgagtca
     1981 gcttccggcg gtagttacaa cggtgttgct ccatatacca gcttgctatt acatcgggca
     2041 tccggcatca aagacattat tcatctgatc aggctgctat cccgcaccga tccccaggat
     2101 gagcagcttg tacaagtgct tgcgcatttt gtccgaatgc ctgttgccga cgtaaaaaaa
     2161 tggtgccgat tattcggtat cagcaattcg ttacttcgcg gattgttaaa tcacgcatcc
     2221 tcccttgggc gcgacggctt tgatgagata gcgcaggcga taaaaaacgg agatatgcca
     2281 ccagctattg actggttttc cattcgccca accagggtga aagcattcct tagcgcggcg
     2341 cattcggcat caccattggc agaaatggtt cagaggttgt cgctcatatt cacagaccat
     2401 accgcgttgg gtgatctgac tctggacgag atgaaagaag cctccattca gtgggccgat
     2461 caacaaaatg aggttaactc tgacttcttg ccagcattca ggaaggccgt tagtaaagtg
     2521 gatgatgccc gtggaattct gaaggcattt aaggcattgc aaagtcgggt taataaacat
     2581 gtcggtgata tcgatggggt aacggcggaa ggcagggata tccttaaaga gtacggcata
     2641 acgccagagt ttattgatga gatcaggact gatatgcagc gtgaggtcgt atcgtccctg
     2701 caaattgtag ccagagcgtt ggcggatgct aatccgaaga gtgcggccat tgttaaccgg
     2761 gttattggtg atattgaagc atcggagggc atgggggcgc tgaaactctt cctttcgcga
     2821 gcgtttaatc ctaacggcaa tattctccct ggcattattg gtgaggctaa aaggtatgtc
     2881 agtgaagaag aacttgagca gcttgaccaa ctacttaagc gattctcata taacccgcag
     2941 acacgctggc aaatgaatca gcgaagaatg ggttcggtcc acgagaaagt gttatctgcc
     3001 atgaacagtg cgatcgcaaa ctcatccgta tctgaagaaa aagctcttga gtgggccgac
     3061 tcttttatca cggaagaagt ggaagaagcc cgcgctggac agaatggtgg gatagacctg
     3121 cgcaaggaac ttgctgatat ttatcgcctg accggcggta aaatttcgac cttatcaaag
     3181 gtggttcacc accagggaag ggcatatgca aatcttaatg gtgttgttgc tgtcaatttg
     3241 aacgatgaaa atgcaagtgc actgtggcac gagctgggtc atcatcttga gtacagtaac
     3301 cctggtttgt tagagaaagc ccggtcattc ctgaaggcca atgttgaagg ggataagcca
     3361 tctttcgtta atatcggtgg gcgtggcaag cctgaatggt gcttcagatc tcgattgagt
     3421 aatatttata tggcgaaggt atacccgcca gcctcagtaa gtaacaccgg gaaaattcgg
     3481 cagaaatcac cgactatttc caaaacgtca gcaacggaag tattctctat ggctcttcag
     3541 ttgtatcatg acaaagaggc cgctgccgca tcactgatga atggtgacgg attgctggaa
     3601 ctgttattag gtgtggcaaa ggagctaaat aatgcagatt aaaatcgcag cgccattagg
     3661 cggagatgcc attatcgaat ttgatgataa tgaagaagtt tccgggcgtt taagcattat
     3721 ctccggtgac attaccgagg acatgatcgc tgaagccata gctggggcaa atcccaatag
     3781 ctatatggga ttcgttaaca cccttgatgc tcccgcaagt gatgttctcc gaacgctgca
     3841 tctttacgct ggctggtttg ttgattggcc agcagtagat ggtggcgatg atgacgacga
     3901 cgatgatgat tttggtgatc atgtagacca gatcgtatat taactccctg attgtgcaaa
     3961 aataatctgt tctgatatgt taattgtgta cttaaagtaa acgcgtagtg gcttgcttta
     4021 ggttatggaa gcaagccgtt acctaatatg ttagatcaaa aaaaattaat ttttgcgttt
     4081 cgctcaccac atattgaaca ctttagtcga tcttttaatt cttccagagt caat
//
LOCUS       DAJOWB010000235         4111 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJOWB010000235 DAJOWB010000000
VERSION     DAJOWB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.236"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..134)
                     /gene="tuf"
                     /locus_tag="OHO85_004742"
     CDS             complement(<1..134)
                     /gene="tuf"
                     /locus_tag="OHO85_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCQ2673976.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     R"
     gene            complement(249..324)
                     /locus_tag="OHO85_004743"
     tRNA            complement(249..324)
                     /locus_tag="OHO85_004743"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(289..291),aa:Thr,seq:ggt)
     gene            complement(331..405)
                     /locus_tag="OHO85_004744"
     tRNA            complement(331..405)
                     /locus_tag="OHO85_004744"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Gly,seq:tcc)
     gene            complement(522..606)
                     /locus_tag="OHO85_004745"
     tRNA            complement(522..606)
                     /locus_tag="OHO85_004745"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(570..572),aa:Tyr,seq:gta)
     gene            complement(615..690)
                     /locus_tag="OHO85_004746"
     tRNA            complement(615..690)
                     /locus_tag="OHO85_004746"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(655..657),aa:Thr,seq:tgt)
     gene            1052..2002
                     /gene="coaA"
                     /locus_tag="OHO85_004747"
                     /gene_synonym="panK"
     CDS             1052..2002
                     /gene="coaA"
                     /locus_tag="OHO85_004747"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCQ2673977.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2031..2996)
                     /gene="birA"
                     /locus_tag="OHO85_004748"
     CDS             complement(2031..2996)
                     /gene="birA"
                     /locus_tag="OHO85_004748"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCQ2673978.1"
                     /translation="MKDYTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(2993..4021)
                     /gene="murB"
                     /locus_tag="OHO85_004749"
     CDS             complement(2993..4021)
                     /gene="murB"
                     /locus_tag="OHO85_004749"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCQ2673979.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAEALLSQFPTAPNYPQADGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1065 a         1022 c          913 g         1111 t
ORIGIN      
        1 cgagcagcac cgccgtaggt tttagccagt acggtagtga ttgcagcggt cagcgttgtt
       61 ttaccatggt caacgtggcc gatagtaccg acgttaacgt gcggttttgt acgttcaaac
      121 ttttctttag acatcgattg tccctctaag acacggataa atcggtgata tcaccacatc
      181 aaccaggcaa catgcccgac ttgttgaatg caataaacag aaggaaaaaa cagggaggag
      241 aaaagaagtg gtgctgatag gcagattcga actgccgacc tcacccttac caagggtgcg
      301 ctctaccaac tgagctatat cagcacatct tggagcgggc agcgggaatc gaacccgcat
      361 catcagcttg gaaggctgag gtaatagcca ttatacgatg cccgcatcct ggaactcggc
      421 tacctgattt tcattctgca ctgaatatcg agagaagctc tctttattcg agccggtaag
      481 cgaacttatc gtctcgggct acgccatcgc gtggccgaaa ttggtggtgg gggaaggatt
      541 cgaaccttcg aagtctgtga cggcagattt acagtctgct ccctttggcc gctcgggaac
      601 cccaccggac ttgatggtgc cgactaccgg aatcgaactg gtgacctact gattacaagt
      661 cagttgctct acctactgag ctaagtcggc atcaagtagc gcgcattcta tggagacatg
      721 cgagttcatg caactaaaaa attgcataat ttgttttatt ggtcacattt tatgcgacac
      781 gatgaagaaa cagccgaacg gagggcagat tagcacactt tttcaacatc attgtgctca
      841 acaatgcgct cctgctaaac cataattctt tttatcagat ggaatatctg tcacattgct
      901 tttcaacgat agcttcctgg cagagatttt ttcttattat tcctccccat ctggtgttac
      961 cctcctgccc attaacccat tcaacagaac tgtgacgcgc catggcaaat atcgctttgc
     1021 cgatagagct atgaccgcca gaaacatgct tatgagtata aaagagcaaa cgttaatgac
     1081 gccttaccta cagtttgacc gcaaccagtg ggcagctctg cgtgattccg tacctatgac
     1141 gttatcggaa gatgagatcg cccgtctcaa aggtattaat gaagatctct cgttagaaga
     1201 agttgccgag atctatttac ctttgtcacg tttgctgaac ttctatataa gctcgaatct
     1261 gcgccgtcag gcagttctgg aacagtttct tggtactaac gggcaacgca ttccttacat
     1321 tatcagtatt gctggcagtg tcgcggtggg gaaaagtaca accgcccgtg tattgcaggc
     1381 gctattaagc cgttggccgg aacatcgtcg tgttgaactg atcactacag atggcttcct
     1441 tcaccctaat caggttctga aagaacgtgg tctgatgaag aagaaaggct tcccggaatc
     1501 gtatgatatg catcgcctgg tgaagtttgt ttccgatctc aaatccggcg tgccaaacgt
     1561 tacagcccct gtttactcgc atcttattta tgatgtgatc ccggatggag ataaaacggt
     1621 tgttcagcct gatattttaa ttcttgaagg gttaaatgtc ttacagagcg ggatggatta
     1681 tccacacgat ccacatcatg tatttgtttc tgattttgtc gatttttcga tatatgttga
     1741 tgcaccggaa gacttacttc agacgtggta tatcaaccgt tttctgaaat tccgcgaagg
     1801 ggcttttacc gacccggatt cctattttca taactacgcg aaattaacta aagaagaagc
     1861 gattaagact gccatgacat tgtggaaaga gatcaactgg ctgaacttaa agcaaaatat
     1921 tctacctact cgtgagcgcg ccagtttaat cctgacgaaa agtgctaatc atgcggtaga
     1981 agaggtcaga ctacgcaaat aattttcagg ggagctaatg ctcccctttc ttatttttct
     2041 gcactacgca gggatatttc accgcccatc cagggtttta ttattccatc ctgctcaagt
     2101 aataaagccc cctgtttgtc tattccgcgt gaaatgccaa atatttcttt atcaccaatg
     2161 ataagtttca ctgggcgatt aataaaatta tccagctttt cccagcgcga cagataaggt
     2221 gccaatcctt cttgttcgaa gagttccaac gcagcacgta attcacgtat tagcatggcc
     2281 gccaacgtat tacgatcgag attgatcccc gcttcctgca gcgtgatcca cccctgatta
     2341 acgacactct cttcaacacg gcgcattgcc atgttgatcc cggctccaat gactatttgc
     2401 gccgcatcgc cagttttgcc agtcagctcc acaagaatgc ctgccagctt gcgatcctgc
     2461 agatagaggt cattaggcca tttgacacga actttatctg ctcccagctt gcgtaatact
     2521 tccgccatca cgataccgat aaccagactt aaaccaatcg ccgccgccgg gccttgttcc
     2581 agacgccaga acatcgacaa atataagttt gcgccaaaag gcgaaaacca tttccgcccc
     2641 cggcgaccac ggccagcctg ctggtattct gcaacacagg catcgcccga tttaagctct
     2701 ccgatacgat caagaaggta ctgattcgtg gagtcaataa ctggtagcac ggctacacta
     2761 ccgccatcca gctgacccaa tatctgttca gcattaagta actggatggg ctcaggcagg
     2821 ctgtatcctt tacccggaac ggtaaagaca tcaacgcccc agtcacgcag tgtctgaatg
     2881 tgtttattaa tagccgcccg gctcattccc agcgtttcac ccaactgctc gccagagtga
     2941 aattcaccgt tcgctaacag ggcaatcaat ttcagtggca cggtgtaatc cttcatgaaa
     3001 ttgtctccac tgcgctcact tcacctgatg caccaataaa gcggacttca ggctcaagcc
     3061 agacattaaa tttttcaccc actttttgtc ttacatggtg tgccagttgc accacatctt
     3121 cgctttttgc attgtcttca ttaatgagaa ctaacgcctg ttgacggtgc accgcagccc
     3181 cacccatttg catccctttt agctggcact gatcaataag ccaacctgct gccagtttta
     3241 ctgaaccatc cgcctggggg taatttggtg ctgttggaaa ttgtgacagt aatgcttcag
     3301 ccgtttcggc agatacaaca gggtttttga agaaactacc ggcattgcca ttcacttttg
     3361 gatcagggag tttggtggtg cgcatatgac acaccgcatt aaatacttgc tgtggcgtta
     3421 ctgttgtagg atccagacga gttaagtcac catacgttag tacaggttgc cactcttttg
     3481 gcagacgcag acctacggct acaatggcga agcgatcctg gtattcatgt ttaaaaatac
     3541 tgtcgcgata gccaaaacgg cactctttgg cagttaagcg cacttgcttg cctgtcgcca
     3601 gttcaacaca atcaacataa gcgcaaactc gctgtaattc tacgccataa gcaccaatat
     3661 tctggatagg tgatgagccg acacaaccag gaattaatgc cagattttcc agaccaggca
     3721 taccttcctg caaagtgtat tttaccaggc gatgccagtt ttctccggct cctacatgta
     3781 aataccacgc atcaggttca tcatgaattt cgataccttt gatccggttg atgatcaccg
     3841 tgccgcgata gtcttccaga aaaagtacat tacttccttc acccagaata agaacgggtt
     3901 gtccttctgc ggttgcatgc tgccaggcat tgagtaattg ttgttcgtct tcggcacata
     3961 caatgtgctg agcattatga tcaatgccaa atgtgttcca gggttttaag gagtggttca
     4021 tagctgcttt cctgatgcaa aaacgaggct agtttaccgt atctgtgggg ggatggcttg
     4081 tagatatgac gacaggaaga gtttgtagaa a
//
LOCUS       DAJOWB010000236         4038 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJOWB010000236 DAJOWB010000000
VERSION     DAJOWB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.237"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(102..500)
                     /gene="vapC"
                     /locus_tag="OHO85_004750"
     CDS             complement(102..500)
                     /gene="vapC"
                     /locus_tag="OHO85_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCQ2673980.1"
                     /translation="MLKFMLDTNICIFTMKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHSGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVDGLRIEDWS"
     gene            complement(500..727)
                     /gene="vapB"
                     /locus_tag="OHO85_004751"
     CDS             complement(500..727)
                     /gene="vapB"
                     /locus_tag="OHO85_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCQ2673981.1"
                     /translation="METTVFFSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHNVSADFMDNREQPGMQERESF"
     gene            809..>4038
                     /gene="traI"
                     /locus_tag="OHO85_004752"
     CDS             809..>4038
                     /gene="traI"
                     /locus_tag="OHO85_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCQ2673982.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGKLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSVMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMAFTPGNTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GRPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSNSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPASLPVSDSPFMALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVG"
BASE COUNT          902 a         1042 c         1280 g          814 t
ORIGIN      
        1 gatatccagc agaagatgca gcgtcgtgaa gaggtgaaca tcaatgtgca ccgggagcgc
       61 ggggagaatg ttgagccggg agatgatttc tgatgaacag gtcagctcca gtcttcaatt
      121 ctcagaccgt ccacacgttc aaattcccgg gtgttattag tcacaataat cagtccccga
      181 ctgcgggcat gacctgcgat catttgatca aatggcccga cagggcgtcc ctgacgggca
      241 agttctgctc ttatctggcc gctgtgtgtg gctgcggcag cgtcgtaatc cagaacatca
      301 atgcgggaaa caaatccctc gatcacagcg agattacgtt caggcatctg gcttttttct
      361 gcaccatata tcagctccat cagggtgacc gaactgatac acattctccc ctggttcagg
      421 ttaaaacgct ccctgacgct ggcgggtttg ttcttcatcg tgaaaatgca gatgttggta
      481 tcgagcataa acttcagcat cagaatgact ccctttcctg catgccgggc tgttccctgt
      541 tatccataaa atcggcgctg acgttgtgtc cgtcgaacca ttcgtcccat gtctctccgg
      601 ctggcgtaat gattctggtt cgtccgacag caatcacttc aacgcgtttt acgttttccg
      661 gcaatgcaac cgcttttggc agtctgaccg cctggctgcg gttgctgaaa aatacggtgg
      721 tttccattat ttgcctcctt atgtggatat gtcttgtgta tatccattgt gggattgata
      781 ccgggatatg tcaaagggat atacgtttat gatgagtatc gcgcaggtca gatcggccgg
      841 aagtgccggt aactattata ccgacaagga taattactat gtgctgggca gcatgggaga
      901 acgctgggcc ggcagggggg ctgaacagct ggggctgcag ggcagcgtcg ataaggatgt
      961 ttttacccgt cttctggagg ggaagctgcc ggacggagcg gatttaagcc gcatgcagga
     1021 tggcagtaat aagcatcgtc ccggctacga cctgaccttc tccgccccca aaagtgtctc
     1081 cgtgatggcc atgttaggcg gtgataaacg tcttattgat gcacataacc aggccgtgga
     1141 ttttgctgtc cgtcaggtgg aggcgctggc ttccacacgg gttatgacgg acggacagtc
     1201 agaaacggtg ctgaccggta atcttgtgat ggcactgttt aaccacgaca ccagtcgcga
     1261 tcaggaacca cagttacaca cacatgcggt agtggccaat gtcacgcagc ataacggcga
     1321 gtggaagaca ctgagcagtg acaaagtggg gaaaacgggg ttcattgaga atgtgtacgc
     1381 taatcagatt gccttcggca ggctctaccg ggaaaaactg aaagagcagg ttgaggcgct
     1441 gggctatgaa acggaagtgg ttggtaagca cggtatgtgg gagatgccgg gcgtaccggt
     1501 ggaggccttc tccggacgct cacaggctat ccgggaggcc gtcggggaag acgcctcgct
     1561 gaaatcccgg gatgtggcgg cactggatac gcgaaaatcc aaacagcacg tcgatcctga
     1621 agtcagaatg gctgaatgga tgcagacgct gaaggaaacc gggttcgaca tccgggcgta
     1681 tcgtgacgcg gcggaccaac gggcggagac ccgcacgcag acgcccggac ccgcttcaca
     1741 ggacgggccg gatgtgcagc aggcggtgac acaggccatt gccggattaa gtgaacgcaa
     1801 agtgcagttt acgtacacgg acgtgctggc cagaacggtc ggcatactgc cgccggaaaa
     1861 tggtgtgatt gaacgggcac gcgccggtat cgatgaggcc atcagccgtg agcagcttat
     1921 ccccctcgat cgtgagaagg ggctgttcac gtccgggatt catgtgctcg atgagctgtc
     1981 agtccgggca ctcagtcgtg acatcatgaa acagaaccgg gtgaccgtac atccggagaa
     2041 aagtgtcccc cggacggccg gttacagcga tgcggtcagt gttctggcac aggatcgccc
     2101 gtcgctggct attgtgtccg ggcagggcgg tgcagccggg cagcgtgagc gggtggcgga
     2161 gctggtcatg atggcccggg agcaggggcg ggaggtgcag attatcgccg ctgaccgtcg
     2221 ctcgcagatg aacctgaagc aggatgaacg gctgtccggt gagctgataa ccggacgtcg
     2281 tcagctgcag gagggcatgg ccttcacgcc gggcaatact gttatcgttg accagggcga
     2341 aaaactctcc ctgaaagaga cgttaaccct gctggacggt gccgcacgtc ataacgtaca
     2401 ggtcctgata accgacagcg ggcagcgaac cggtacaggc agtgcactga tggccatgaa
     2461 agatgccggg gtgaacacat atcgctggca ggggggagaa cagcggccgg ccaccatcat
     2521 cagtgaaccg gaccgtaatg tccgctatgc ccggctggca ggagattttg ctgccagcgt
     2581 gaaagccgga gaagagagcg tggcacaggt cagcggggta cgggaacagg ccatactgac
     2641 acaggccatt cgcagtgagc tgaaaacaca gggcgtgctc ggacgcccgg aggtaaccat
     2701 gaccgccctg tcgccggtct ggctggacag ccggagccgt tatctgcggg atatgtaccg
     2761 tccggggatg gtgatggagc agtggaaccc ggagacacgc agtcatgacc gctatgttat
     2821 cgaccgggtg acggcgcaga gtaacagcct gaccctgcgg gatgcgcagg gtgaaacgca
     2881 ggtggtgcgt atttcctccc tggacagcag ctggtcgctg ttccggccgg aaaaaatgcc
     2941 ggtggcagac ggcgagcgac tgagggtgac agggaaaatt cccggactcc gcgtctccgg
     3001 cggtgaccgc ctgcaggtgg catccgtcag tgaagatgcg atgacggttg ttgtgccggg
     3061 gcgggctgaa ccggcttccc tgcctgtgag cgattcacca ttcatggcac tgaagctgga
     3121 gaacggctgg gtggaaacgc ccgggcattc cgtcagcgac agtgcgaagg tttttgcctc
     3181 cgtcacacag atggcaatgg acaacgccac cctgaacggt ctggcccgca gcggtcgtga
     3241 tgtccggctg tattcctcac tggatgaaac ccgtactgcg gaaaaacttg cccgccatcc
     3301 ctcctttacg gtggtttctg agcagataaa ggcgcgtgcc ggtgagacat tgctggaaac
     3361 cgctatcagt ctgcagaaag ccgggcttca cacgccggca cagcaggcta ttcatctggc
     3421 ccttcctgtg ctggaaagta aaaacctggc cttcagcatg gtggacctgc tgacagaggc
     3481 gaagtcgttt gctgcagaag gaaccagttt tactgaactg ggaggggaaa tcaatgcgca
     3541 gataaaacgc ggtgatttac tgtatgtgga tgtggcaaaa ggctatggca caggcctgct
     3601 ggtttcccgt gcgtcgtatg aggcagaaaa gagcattctt cgccatattc tcgaaggtaa
     3661 ggaggcggtc acgccgctga tggagagagt acctggcgaa ctcatggaga aactgacatc
     3721 aggacagcgt gccgccaccc gcatgatact ggaaacgtcc gaccgtttca cggtggtgca
     3781 gggctatgcc ggtgtgggta agaccacaca gttccgggcg gtgatgtcag ccgtgaacat
     3841 gctgccggag agtgagcgtc cccgtgtcgt tgggctgggt cccacgcacc gcgcggtcgg
     3901 ggagatgcgc agcgccggcg tggatgcaca gacactggcg tcctttctgc atgacacgca
     3961 gctgcagcag cgcagcggag aaacgccgga tttcagcaac acgctgttcc tgctcgatga
     4021 gagctcaatg gtgggcaa
//
LOCUS       DAJOWB010000237         4007 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJOWB010000237 DAJOWB010000000
VERSION     DAJOWB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.238"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1227
                     /gene="ag43"
                     /locus_tag="OHO85_004753"
     CDS             <1..1227
                     /gene="ag43"
                     /locus_tag="OHO85_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCQ2673983.1"
                     /translation="GRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAF
                     IQGNKLQAGAFNYSLNRDSDESWYLRSENAYRAEVPLYTSMLTQAMDYDRILAGSRSH
                     QTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMS
                     LTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLVHTSSGLWADIVAQGTRH
                     SMKASSDNNNFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGY
                     VKFGHGSTQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSTKHRVSELPVNWWVQPSVIR
                     TFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLGVQAGYAHSVSG
                     SSAEGYNGQATLNVTF"
     gene            1298..1456
                     /locus_tag="OHO85_004754"
     CDS             1298..1456
                     /locus_tag="OHO85_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCQ2673984.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            1611..2429
                     /locus_tag="OHO85_004755"
     CDS             1611..2429
                     /locus_tag="OHO85_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCQ2673985.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKHDAMQSLVLSPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGQDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            2771..3244
                     /locus_tag="OHO85_004756"
     CDS             2771..3244
                     /locus_tag="OHO85_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCQ2673986.1"
                     /translation="MKTLSQNTTSSACAPETDLQKLVATPVPDERRISFWPQHFGLIP
                     QWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            3260..3736
                     /locus_tag="OHO85_004757"
     CDS             3260..3736
                     /locus_tag="OHO85_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCQ2673987.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEYGLL"
     gene            3799..>4007
                     /locus_tag="OHO85_004758"
     CDS             3799..>4007
                     /locus_tag="OHO85_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCQ2673988.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSV"
BASE COUNT          895 a         1128 c         1116 g          868 t
ORIGIN      
        1 ggcagggcaa ccggaaaaac catcctgaac ctggtgaacg ccggcaacag tgcgtcgggg
       61 ctggcgacca gcggtaaggg tattcaggtg gtggaagcca ttaacggtgc caccacggag
      121 gaaggggcct ttatccaggg gaataagctg caggccggtg cctttaacta ctccctcaac
      181 cgggacagtg atgagagctg gtatctgcgc agtgaaaatg cttatcgtgc tgaagtcccc
      241 ctgtatacat ccatgctgac acaggcaatg gactatgacc ggattctggc aggctcccgc
      301 agccatcaga ccggtgtaaa cggtgaaaat aacagcgtcc gtctcagcat tcagggcggt
      361 catctcggtc acgataacaa cggcggtatt gcccgtggag ccacgccgga aagcagcggc
      421 agctatggct tcgtccgtct ggagggtgac ctgctgagaa cagaggttgc cggtatgtct
      481 ctgacgacag gggtgtatgg tgccgcaggc cattcttccg ttgatgttaa ggatgatgac
      541 ggttcccgcg ccggcacggt ccgggatgat gccggcagtc tgggcggata cctgaatctg
      601 gtacacacgt cctccggcct gtgggctgac attgtggcac agggaacccg ccacagcatg
      661 aaagcgtcat cggacaataa caacttccgc gcccggggct ggggctggct gggctcactg
      721 gaaaccggtc tgcccttcag tatcactgac aacctgatgc tggagccaca actgcagtac
      781 acctggcagg ggctctccct ggatgacggc caggataacg ccggttatgt gaagttcggg
      841 catggcagta cacaacatgt gcgtgccggc ttccgtctgg gcagccacaa cgatatgacc
      901 tttggcgaag gcacctcatc ccgtgacacc ctgcgcgaca gtacaaaaca ccgtgtgagt
      961 gaactgccgg tgaactggtg ggtacagcct tctgttatcc gcactttcag ctcccggggt
     1021 gacatgagca tgggtacagc cgccgccggc agtaacatga cgttctcacc gtcccggaat
     1081 ggcacgtcac tggacctgca ggccggactg gaagcccgtg tccgggaaaa tatcaccctg
     1141 ggcgttcagg ccggttatgc ccacagcgtc agcggcagca gcgctgaagg ttataacggt
     1201 caggccacgc tgaatgtgac tttttgataa tcaggtcact ctccttcagg ccccggtcat
     1261 catgaccggg gctttttact ttcagggagc aacagatatg ccgggttgta catctcgttt
     1321 attgcccgaa ggccctttct cacgcgaaca ggctgttgcc gtcaaaacag cttaccgcaa
     1381 tgtgtttact gaagatgacc agggaacata ttcccggctg gttatccgta atgctgaagg
     1441 tcagttgcgc tggtgatgcc ggaacacagc ttaatactca gctcgccagt taagggcttc
     1501 tcagacaata aacgtcattc atttcatctg tcaggccgcg tcttctccgg gagacgtggc
     1561 tttttcattt attacaccag taaatcgtaa ccaccgataa ggagcaaatt atgcgactag
     1621 ccagtcgttt tggttatgcg aaccagatac gccgtgaccg tccgctgaca cacgaagaac
     1681 tgatgcacta tgttcccagt attttcggtg aagaccggca tacttcccgc agtaaacgat
     1741 atgcgtacat tcccaccatc accgtactgg aaagcctgca gcgggaaggc tttcagccat
     1801 tcttcgcctg ccagacccgt gtgcgcgacc cgggccgccg gggatacaca aaacacatgc
     1861 tgcgtctgcg gcgggccgga gagataaacg gagaacatgt ccctgaaatc attctgctca
     1921 actcccatga cggtacctcc agctaccaga tgctgccggg ttacttcagg ttcgtctgtc
     1981 agaacggatg cgtctgtggt cagtctctgg gggaagtgcg tgttccgcac cggggaaatg
     2041 tggtggacag agtcattgaa ggggcttacg aggtggtggg cgtgtttgac cggatagagg
     2101 agaagcatga tgccatgcag tcgctggtcc tgtcgccacc ggcacgccag gcgctggcac
     2161 aggcggcact gacttaccgt tatggtgacg aacatcagcc agtcaccacc gctgacattc
     2221 tgacaccacg acgcagggag gattacggtc aggacctgtg gagcgcatat cagaccatcc
     2281 aggagaatat gctgaaaggc ggaatttccg gccgcagtgc aaaaggaaaa cgtatccaca
     2341 cccgtgccat tcacaacatc gacaccgata ttaagctcaa ccgcgcattg tgggtaatgg
     2401 cagaaacgct gctggagagc ctgcgctgat gcagcatgtc cctgaaagcg tgattcaggc
     2461 accagaatca cccgacgccc cggaggcgct gccggggtgt cgttccattt ctgatgatga
     2521 cctgtgcgcc tggtgtaccc gtctgcttta ccgtcccgga gaaatcagtc tgtgcagact
     2581 gagtatacag gcaatggcat ctggccatcc gtctgtgacc ggaacggtta cgcgtacggc
     2641 tgtccggaat ttcagccgaa tatcattcat ccctgatatt accgggtcat cacggtcagt
     2701 gatgaacgtg atgttgccag ctcccgacct ttactccctc tcatttattc acattacaag
     2761 gatttcatac atgaaaacat tgtctcagaa caccacctct tcggcctgtg caccggagac
     2821 tgacctgcaa aaactggtag ccactcccgt ccctgatgaa cggcgcatca gcttctggcc
     2881 gcagcacttt ggcctcattc cacagtgggt gaccctggag ccccgtatct tcggctggat
     2941 ggaccgtctg tgtgaagact actgcggtgg tatctggaat ctgtacaccc tgaacaacgg
     3001 cggggcattt atggcacccg aaccggatga cgatgatgac gaaacatggg tactgttcaa
     3061 tgccatgaac ggtaaccgcg ctgaaatgag cccggaagcc gccggcattg ctgcctgtct
     3121 gatgacgtac agtcatcatg cctgtcgtac ggagtgttat gccatgacgg tccattatta
     3181 ccggctgcgg gattacgccc tgcagcatcc ggaatgcagc gccattatgc gcatcatcga
     3241 ctgaatggag agaagtacaa tgcaacagct ttcctttctg cccggagaga tgacgcccgg
     3301 cgagcgcagc ctcattcaac gggccctgaa aaccctggac cgccatcttc atgaacccgg
     3361 cgtggccttc acctccaccc gtgcggcacg agaatggctg attctgaaca tggcgggact
     3421 ggagcgtgaa gagttccggg tgctgtatct gaacaaccag aatcagctga ttgccggtga
     3481 aaccctcttc accggcacca tcaaccgcac ggaagtccat ccccgggaag tgattaaacg
     3541 cgccctgtat cacaatgccg ctgctgtggt gctggcacac aatcacccgt ccggtgaagt
     3601 cacacccagc aaggcagacc gccttatcac ggaacgtctg gtacaggcac tgggcctggt
     3661 ggatatccgg gtgccggacc atctgatagt cggtggcagc caggttttct cctttgccga
     3721 atatggtctg ctttaacccg tcacaaccac atcacacctg ttttcacttt tatcttctgt
     3781 cttcagaggt atcccactat gaaaatcatc acccgtggcg aagccatgcg tattcaccgt
     3841 cagcatccgg catcccgtct ttttccgttc tgtaccggca aataccgctg gcacggcagt
     3901 gctgaagcgt ataccggtcg tgaagtgcag gatattcccg gtgtgctggc cgtgtttgca
     3961 gaacgccgta aggacagttt tggtccgtat gtccggctga tgagcgt
//
LOCUS       DAJOWB010000238         3997 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJOWB010000238 DAJOWB010000000
VERSION     DAJOWB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.239"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            230..550
                     /locus_tag="OHO85_004759"
     CDS             230..550
                     /locus_tag="OHO85_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCQ2673989.1"
                     /translation="MIAVIFEVQIQPDQQTRYLTLAEELRPLLSHVAGFISIERFQSL
                     ATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGRESIFDFYKISIAHITREYSFKKD
                     KDNV"
     gene            543..929
                     /locus_tag="OHO85_004760"
     CDS             543..929
                     /locus_tag="OHO85_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCQ2673990.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            937..1623
                     /locus_tag="OHO85_004761"
     CDS             937..1623
                     /locus_tag="OHO85_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCQ2673991.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1601..2224)
                     /gene="tetR(B)"
                     /locus_tag="OHO85_004762"
     CDS             complement(1601..2224)
                     /gene="tetR(B)"
                     /locus_tag="OHO85_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCQ2673992.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFNHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2306..3511
                     /gene="tet(B)"
                     /locus_tag="OHO85_004763"
     CDS             2306..3511
                     /gene="tet(B)"
                     /locus_tag="OHO85_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCQ2673993.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLMSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3624..3812)
                     /locus_tag="OHO85_004764"
                     /pseudo
     CDS             complement(3624..3812)
                     /locus_tag="OHO85_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3828..>3997)
                     /locus_tag="OHO85_004765"
     CDS             complement(3828..>3997)
                     /locus_tag="OHO85_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCQ2673994.1"
                     /translation="KLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKV
                     IGHYLNIKHYQ"
BASE COUNT         1116 a          739 c          871 g         1271 t
ORIGIN      
        1 aataccatgc gggatccgca ctattttcgg tgatagcaaa aaataaaaaa gtataaataa
       61 taaacaaaat tataagagaa ggagaacatg ataagcgcag gccgagcaat ttaaagaagt
      121 gtgatataca acataaaaca gcagcatgac ctttctatat ttcgttgctt atcgaagtgt
      181 taattttcgg gtggattact ctgtttgttg attaataacg gagaaaaata tgattgctgt
      241 aatatttgag gtgcaaatac aacccgacca acaaactcgc tatttgactt tagctgagga
      301 gttaagacca ctattaagtc atgtagctgg ttttatttca attgaacgtt ttcaaagtct
      361 agctacagaa ggaaaaatgt tatcgctatc ttggtgggaa aacgaatacg cagttctgca
      421 atggaaaaat catgttttac atgcgaaagc tcaacaagaa gggcgagagt caatatttga
      481 tttttacaaa attagtattg ctcatattac tcgcgaatat tcatttaaaa aggacaagga
      541 taatgtttga tgttcacgtt gttttagata atcaaatagg acaattagca ttactaggaa
      601 aaacattagg taataaaggt attggattgg aagggggagg gatatttacg gttggtgatg
      661 aatgccatgc tcattttctt gttgaacaag gaaaggaagc taaaatagcg ctagagcaag
      721 ctggactgtt agtacttgcg atccggacac cattaattcg taagttaaaa caggaaaaac
      781 cgggggaact tggcgaaata gcacgagtat tggcggagaa taacattaat attttagtgc
      841 aatacagtga ccatgctaac caactgatat taataacgga caatgatagt atggctgcat
      901 ctgttacgct cccttgggca ataaagtgaa cttgcgatgg ctaatttaat acgaaaagag
      961 gttacctttg agtcctcaat agccgcgata ggggcggcta tgtctgacat ttcacgagtt
     1021 aaaatactca gtgctttgat ggatgggcga gcttggacgg ccactgagct aagttctgtg
     1081 gcgaatatat cagcttcaac ggcgagcagt catttatcta aattattaga ttgccagcta
     1141 atcacagtag tagctcaagg caagcatcgt tattttcggc tagcaggaaa agatattgct
     1201 gaattgatgg aaagtatgat ggggatctcc ttaaaccatg gcgtacatgc caaagtttcc
     1261 acgccagtgc atttacgaaa agcacgtact tgctatgatc atttagctgg cgaagttgcc
     1321 gttaagatct atgattccct ttgtcaacag caatggatca ctgaaaatgg ttcaatgatc
     1381 acattaagtg gtattcaata ttttcatgaa atgggaattg acgttccttc caaacattca
     1441 cgtaaaatct gttgtgcgtg tttagattgg agtgaacgcc gtttccattt aggtgggtac
     1501 gttggagccg cattattttc gctttatgaa tctaaagggt ggttaactcg acatcttggt
     1561 taccgtgaag ttaccatcac ggaaaaaggt tatgctgctt ttaagaccca ctttcacatt
     1621 taagttgttt ttctaatccg catatgatca attcaaggcc gaataagaag gctggctctg
     1681 caccttggtg attaaataat tcgatagctt gtcgtaataa tggcggcata ctatcagtag
     1741 taggtgtttc cctttcttct ttagcgactt gatgctcttg atcttccaat acgcaaccta
     1801 aagtaaaatg ccccacagcg ctgagtgcat ataatgcatt ctctagtgaa aaaccttgtt
     1861 ggcataaaaa ggctaattga ttttcgagag tttcatactg tttttctgta ggccgtgtac
     1921 ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc acatctaaaa cttttagcgt
     1981 tattacgtaa aaaatcttgc cagctttccc cttctaaagg gcaaaagtga gtatggtgcc
     2041 tatctaacat ctcaatggct aaggcgtcga gcaaagcccg cttatttttt acatgccaat
     2101 acaatgtagg ctgctctaca cctagcttct gggcgagttt acgggttgtt aaaccttcga
     2161 ttccgacctc attaagcagc tctaatgcgc tgttaatcac tttactttta tctaatctag
     2221 acatcattaa ttcctaattt ttgttgacac tctatcattg atagagttat tttaccactc
     2281 cctatcagtg atagagaaaa gtgaaatgaa tagttcgaca aagatcgcat tggtaattac
     2341 gttactcgat gccatgggga ttggccttat catgccagtc ttgccaacgt tattacgtga
     2401 atttattgct tcggaagata tcgctaacca ctttggcgta ttgcttgcac tttatgcgtt
     2461 aatgcaggtt atctttgctc cttggcttgg aaaaatgtct gaccgatttg gtcggcgccc
     2521 agtgctgttg ttgtcattaa taggcgcatc gctggattac ttattgctgg ctttttcaag
     2581 tgcgctttgg atgctgtatt taggccgttt gctttcaggg atcacaggag ctactggggc
     2641 tgtcgcggca tcggtcattg ccgataccac ctcagcttct caacgcgtga agtggttcgg
     2701 ttggttaggg gcaagttttg ggcttggttt aatagcgggg cctattattg gtggttttgc
     2761 aggagagatt tcaccgcata gtcccttttt tatcgctgcg ttgctaaata ttgtcgcttt
     2821 ccttgtggtt atgttttggt tccgtgaaac caaaaataca cgtgataata cagataccga
     2881 agtaggggtt gagacgcaat cgaattcggt atacatcact ttatttaaaa cgatgcccat
     2941 tttgttgatt atttattttt cagcgcaatt gataggccaa attcccgcaa cggtgtgggt
     3001 gctatttacc gaaaatcgtt ttggatggaa tagcatgatg gttggctttt cattagcggg
     3061 tcttggtctt ttacactcag tattccaagc ctttgtggca ggaagaatag ccactaaatg
     3121 gggcgaaaaa acggcagtac tgctcggatt tattgcagat agtagtgcat ttgccttttt
     3181 agcgtttata tctgaaggtt ggttagtttt ccctgtttta attttattgg ctggtggtgg
     3241 gatcgcttta cctgcattac agggagtgat gtctatccaa acaaagagtc atcagcaagg
     3301 tgctttacag ggattattga tgagccttac caatgcaacc ggtgttattg gcccattact
     3361 gtttgctgtt atttataatc attcactacc aatttgggat ggctggattt ggattattgg
     3421 tttagcgttt tactgtatta ttatcctgct atcgatgacc ttcatgttaa cccctcaagc
     3481 tcaggggagt aaacaggaga caagtgctta gttatttcgt caccaaatga tgttattccg
     3541 cgaaatataa tgaccctctt gataacccaa gagggcattt tttacgataa agaagattta
     3601 gcttcaaata aaacctatct attttattta tctttcaagc tcaataaaaa gccgcggtaa
     3661 atagcaataa attggccttt tttatcggca agctctttta ggtttttcgc atgtattgcg
     3721 atatgcataa accagccatt gagtaagttt ttaagcacat catcatcata agctttaagt
     3781 tggttctctt ggatcaattt gctgacaatg gcggtaatga ctccaactta ttgatagtgt
     3841 tttatgttca gataatgccc gatgactttg tcatgcagct ccaccgattt tgagaacgac
     3901 agcgacttcc gtcccagccg tgccaggtgc tgcctcagat tcaggttatg ccgctcaatt
     3961 cgctgcgtat atcgcttgct gattacgtgc agctttc
//
LOCUS       DAJOWB010000239         3975 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJOWB010000239 DAJOWB010000000
VERSION     DAJOWB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.240"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            202..1908
                     /locus_tag="OHO85_004766"
     CDS             202..1908
                     /locus_tag="OHO85_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673995.1"
                     /translation="MSNLREYQNRIADIAKRSKAVLGWASTAQFGTDNQFIKDDAARA
                     ASILEAARKDPVFAGISDNATAQIATAWASALADYAAAHKSMPRPEILASCHQTLENC
                     LIESTRNSMDATNKAMLESVAAEMMSVSDGVMRLPLFLAMILPVQLGAATADACTFIP
                     VTRDQSDIYEVFNVAGSSFGSYAAGDVLDMQSVGVYSQLRRRYVLVASSDGTSKTATF
                     KMEDFEGQNVPIRKGRTNIYVNRIKSVVDNGSGSLLHSFTNAAGEQITVTCSLNYNIG
                     QIALSFSKAPDKGTEIAIETEINIEAAPELIPLINHEMKKYTLFPSQFVIAAEHTVQA
                     AYEAQREFGLDLGSLQFRTLKEYLSHEQDMLRLRIMIWRTLATDTFDIALPVNQSFDV
                     WATIIRGKFQTVYRDIIERVKSSGAMGMFAGADAASFFKQLPKDFFQPAEDYIQTPYV
                     HYIGTLFGNVKVYEVPAGICKNLTTENIQFSSMDVLCYVRDENPGKAGFVTGDAVPAI
                     PFQHPTTPALVNRTTQWGSAINDMHPRNGADYFTRVTLTMAKKGGLNFISGDTIDAGD
                     SE"
     gene            1969..3558
                     /locus_tag="OHO85_004767"
     CDS             1969..3558
                     /locus_tag="OHO85_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673996.1"
                     /translation="MSQYSIQQSLGNASGVAVSPINADATLSTGVALNSSLWAGIGVF
                     ARGKPFTVLAVTESNYEDVLGEPLKPSSGSQFEPIRHVYEAIQQTSGYVVRAVPDDAK
                     FPIIMFDESGEPAYSALPYGSEIELDSGEAFAIYVDDGDPCISPTRELTIETATADSA
                     SNERFILKLTQTTSLGVVTTLETHTVSLAEEAKDDMGRLCYLPTALEARSKYLRAVVN
                     EDLISTAKVTNKKSLAFTGGTNGDQSKISTAAYLRAVKVLNNAPYMYTAVLGLGCYDN
                     AAITALGKICADRLIDGFFDVKPTLRYAEALTAVEGTGLLGTDYVSCSVYHYPFSCKD
                     KWTQSRVVFGLSGVAYAAKAHGVKKNSDVGGWHYSPAGEERAVIARASIQPLYPEDTP
                     DEEAMVKGRLNKVSVGTSGQMIIDDALTCCTQDNYLHFQHVPSLMNAISRFFVQLARQ
                     MKHSPDGITAAGLTKGMTKLLDRFVASGALVAPRDPDADGTEPYVLKVTQAEFDKWEV
                     VWACCPTGVARRIQGVPLLIK"
     gene            3568..>3975
                     /locus_tag="OHO85_004768"
     CDS             3568..>3975
                     /locus_tag="OHO85_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2673997.1"
                     /translation="MSKNFFQSGAFLGNGLSRFALNSDPVQLMESARASAEPPTDPVI
                     NNNPEPAAQTNDNVPSAPAPEQILEGKDGKEWTVEQAHQMILEAANRSAMQNALSDAA
                     DAVFAWADSGDLTFDSLDGFVQAIAGISDDDDAE"
BASE COUNT          967 a          995 c         1002 g         1011 t
ORIGIN      
        1 ttaaccaact gtttttaaag taaatccatc taaaatttca aggtgaaatc gccacgattt
       61 caccttggat tttaccttcc tcccctactc ccgaaaaaaa taaaaaaatt gcttgtcacg
      121 agaaagtcaa caagtgactt tcaataaaat ctcttccgaa aagggattca cacaagtgcc
      181 ttgtgtttaa ggaagagtaa attgagtaac ttacgcgaat accagaatcg tattgcagat
      241 atcgcaaaac gctctaaagc tgtgcttggc tgggcaagca ctgcgcagtt cggtactgat
      301 aaccaattca ttaaagatga tgccgcgcgt gccgcatcta tccttgaagc tgcacgtaaa
      361 gacccggttt ttgcgggtat ctctgataat gccaccgctc aaatcgctac agcgtgggca
      421 agtgcactgg ctgactacgc cgcagcacat aaatctatgc cgcgtccgga aattctggcc
      481 tcctgccacc agacgctgga aaactgcctg attgagtcca cccgcaatag catggatgcc
      541 actaataaag cgatgctgga atccgtcgca gcagagatga tgagcgtttc tgacggtgtt
      601 atgcgtctgc ctttattcct cgcgatgatc ctgcctgttc agttgggggc agctaccgct
      661 gatgcgtgta ccttcattcc ggttacgcgt gaccagtccg acatctatga agtctttaac
      721 gtggcaggtt cctcttttgg ttcttatgct gctggtgatg ttctggacat gcaatccgtc
      781 ggtgtgtaca gccagttacg ccgccgctat gtgctggtgg caagctccga tggcaccagc
      841 aaaaccgcaa ccttcaagat ggaagacttc gaaggccaga atgtaccaat ccgaaaaggt
      901 cgcactaata tctacgttaa ccgtattaag tctgttgttg ataacggttc cggcagccta
      961 cttcactcgt ttactaatgc tgctggtgag caaatcactg ttacctgctc tctgaactac
     1021 aacattggtc agattgccct gtcgttctcc aaagcgccgg ataaaggcac tgagatcgca
     1081 attgagacgg aaatcaatat tgaagccgct cctgagctga tcccgctgat caaccacgaa
     1141 atgaagaaat acaccctgtt cccaagccag ttcgttatcg cggctgagca cacggtacag
     1201 gcggcgtatg aagcacagcg tgaatttggt ctggacctgg gttccctaca gttccgcacc
     1261 ctgaaggaat acctgtctca tgaacaggat atgctgcgtc ttcgcatcat gatctggcgt
     1321 actcttgcga ccgacacctt tgacatcgct ctgccggtta accagtcctt tgatgtatgg
     1381 gcaaccatca ttcgtggcaa attccagact gtatatcgcg acattattga gcgcgttaaa
     1441 tcttctggtg cgatggggat gtttgctggt gctgatgcag catctttctt caaacagttg
     1501 ccgaaggatt tcttccagcc agccgaagac tatatccaga ctccgtatgt tcactacatc
     1561 ggtaccctgt tcggtaacgt gaaagtgtac gaagtacctg ctggtatttg taagaactta
     1621 acgacagaga acattcagtt cagctcgatg gatgtgctgt gctacgtccg tgatgaaaat
     1681 ccgggtaaag caggcttcgt gactggtgat gctgtcccgg ccatcccgtt ccagcatccg
     1741 accactccgg cgctggtcaa ccgtaccacg cagtggggtt cggctatcaa cgatatgcac
     1801 ccacgcaacg gcgctgatta cttcactcgt gtaacgctga caatggccaa aaaaggcggg
     1861 cttaacttca taagcggcga cacgattgat gccggtgact ctgagtaatc aggggaagtt
     1921 ctccgtttaa catagcgccc ccgtgcgggg cgcataacag ggaaagttat gtctcaatat
     1981 tcaattcaac agtcattagg taatgcatcc ggcgtcgcgg ttagcccgat caatgccgat
     2041 gcgacgttat ctaccggtgt tgcattaaat agcagcttgt gggctggtat tggcgtattt
     2101 gcgcgtggca agccgtttac tgttctggcg gttactgagt ccaattacga agatgttctc
     2161 ggcgaaccgc tgaagccgtc ttccggctca cagttcgaac caattcgcca tgtgtacgaa
     2221 gctattcagc aaacgtctgg ttatgttgtc cgtgctgttc cggatgatgc gaagttcccg
     2281 attattatgt tcgatgaatc aggcgaaccg gcttacagtg cgttgccata cggttctgaa
     2341 attgaacttg atagcggcga agcctttgct atctacgttg atgatggtga tccgtgtatt
     2401 tcacctaccc gtgagttaac catcgaaacg gcaacagcgg acagcgcgag taatgaacgc
     2461 ttcatcttaa aactgaccca gacgacttcg cttggtgtgg taacgaccct ggagacacac
     2521 actgtgtctt tggcggaaga agcgaaagat gacatgggcc gcttgtgtta tctgcctacg
     2581 gctctggaag ctcgttctaa atatctgcgc gcggttgtta atgaagatct gatttctaca
     2641 gcgaaagtaa caaacaaaaa atcgttggcg ttcactggtg gtaccaacgg tgatcagtcg
     2701 aaaatctcaa ccgctgcgta cctgcgtgcg gtgaaagtgc tgaacaatgc gccgtacatg
     2761 tacaccgctg ttcttggcct gggctgctat gacaatgcgg ctatcaccgc attaggtaaa
     2821 atctgtgcag atcgcctgat tgatggcttc tttgatgtca aaccgacatt aaggtacgca
     2881 gaagcactaa cagctgttga gggtaccggt ttacttggta ccgattatgt aagctgttct
     2941 gtctatcact acccgttctc ctgcaaagac aaatggaccc aatcccgtgt ggtctttggt
     3001 ctgtctggcg tggcgtatgc ggcgaaagct catggcgtca agaaaaactc tgatgtcggc
     3061 ggttggcatt actcaccggc tggtgaagaa cgtgccgtca ttgctcgtgc gtcaattcaa
     3121 ccgctgtatc cggaagatac cccggacgaa gaagcaatgg tcaagggccg tctcaataaa
     3181 gtatctgttg gcacctctgg ccagatgatc atcgacgatg ctttaacttg ctgcacgcag
     3241 gataactatc tgcacttcca gcacgtccca tccctgatga atgcaatcag ccgtttcttt
     3301 gtccagttag cccgacagat gaagcatagc ccggacggta ttactgcggc tggcctgact
     3361 aaagggatga ccaaactttt agatcgcttt gtcgcctccg gcgctctggt ggctcctcgt
     3421 gatcctgatg ctgacggtac agaaccgtat gtgctgaaag ttacgcaggc ggaattcgat
     3481 aaatgggaag tagtctgggc ctgctgcccg actggcgtag cccgtcgtat ccagggcgta
     3541 ccgctgctta ttaagtaagg gaatacaatg agcaaaaact tttttcaatc cggggcattt
     3601 ttggggaatg gactgtcccg tttcgctttg aactctgatc ctgtgcagct gatggagtct
     3661 gcccgagcaa gcgccgaacc gccaacagat ccggttatta ataataatcc ggaaccggcg
     3721 gcacagacta acgataacgt tccatctgcc ccggctcctg agcaaatcct ggaagggaaa
     3781 gacggtaaag aatggaccgt cgaacaggcg caccagatga ttctggaagc tgcaaatcga
     3841 agtgctatgc agaatgcgtt gagtgatgcg gccgacgccg ttttcgcctg ggctgatagc
     3901 ggtgatctga ctttcgactc ccttgatggt ttcgttcagg ctatcgctgg tatctctgat
     3961 gacgacgacg ccgaa
//
LOCUS       DAJOWB010000240         3941 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJOWB010000240 DAJOWB010000000
VERSION     DAJOWB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.241"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..319)
                     /gene="traS"
                     /locus_tag="OHO85_004769"
     CDS             complement(<1..319)
                     /gene="traS"
                     /locus_tag="OHO85_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HCQ2673998.1"
                     /translation="MITHYDIKMETQKLKDVLSVEGVNIPPLLQVIKPGGYVFLWVLL
                     WPTFLRLLADKVDIRDAGFDICFSGVMGFILFVAITNGMMLYLAIPEKFRDESKVISF
                     MYDK"
     gene            complement(316..3141)
                     /gene="traG"
                     /locus_tag="OHO85_004770"
     CDS             complement(316..3141)
                     /gene="traG"
                     /locus_tag="OHO85_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HCQ2673999.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGAEMVSKSTDFLSRHPEIANIFQDY
                     VQNCVMGDIYLNHKYTLEELMASADPYTLIFSRPSPLRGVYDSNNNFVTCKDASVSLK
                     DKLNLDTQSGGKTWHYYAQQLFGGRPDPNLLFSTLIGDSYSYFYGSSKSASQIIRQNV
                     TINALKEGITSYAARNGDSASLVNLATTSSMEKQRLAHVSIGHVAMRTLPMTQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQSWPMLYAILNSAMTFYAKQNGAPV
                     VLSEISQIQLKYSDLASTAGYLSMMIPPLSWMMVRGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINTTRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATRELASRSTTASAGLY
                     GDAHAGLDAKPSLFGVRVDVGAKVGGRASIDGSDLDSHEASSGSRASHDARHDIDARA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQSSIHGEENIVGRQYSELQNHHKTEALSQNNKYDEERES
                     QKIIPEPSGEAVKEMIDGKKERLKGPL"
     gene            complement(3138..>3941)
                     /locus_tag="OHO85_004771"
     CDS             complement(3138..>3941)
                     /locus_tag="OHO85_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HCQ2674000.1"
                     /translation="QGCTVGGKSDSVRDKASDKDKERVTKNINIMWNALSKNRMFDGN
                     KELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGGTAKIYHCNDSDKCLKV
                     VADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGFISSTTIPVFKYLVDPQ
                     MLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYDEAVIGHINDNMNDATR
                     QIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNYHFGGSTL"
BASE COUNT          924 a         1073 c          899 g         1045 t
ORIGIN      
        1 tcttatcata cataaaagat ataactttcg actcatctct gaatttttca ggtatagcaa
       61 gatacaacat cattccatta gtaatggcaa caaaaaggat aaaacccatt acgccactaa
      121 aacatatatc aaatccggca tctcggatat caactttatc tgccagcaaa cgaagaaatg
      181 ttggccataa taaaacccat aaaaacacat acccccctgg ctttatgacc tgtaataatg
      241 gcgggatatt aaccccctct acagagagta catcctttag tttctgtgtc tccattttta
      301 tatcatagtg agtgatcata atggcccctt cagtcgttcc tttttaccat caatcatttc
      361 tttcacagcc tctcctgatg gctcaggtat tatcttttga ctttctcgct cttcatcata
      421 tttattattc tgagataatg cttctgtttt atggtgattt tgtagttcag aatactgtct
      481 tcctacaata ttttcttcgc catgtatgct gctctgagta tccccgatat tgcctcggta
      541 ttctgtgacc atattatcaa cctgatgttt tacatcattt cggatattac tgtcctgcgt
      601 tctttgctca ataatggcct gatgtccctg atggtcagca ataatatcct ggctgccacc
      661 acccgaaggt acgctctcca ttcctttacc aatatcacca cgggattcac gccaggcatt
      721 atcaacacca ggctgcacct gttcctgcac aaaagaccac gccattgccc gacggcgttc
      781 tgcaatctcc ggcgaactgg tatttgtcag aatagcttct gcatcctgcg gagcgtgttt
      841 catcacatac tgtgcaaact gttgcgatag gtcttcactc atctgcccac tcatgctttc
      901 agtacgggat gccatttcag catattcatg gctgcgggtc atattcgtcg tgtactggtc
      961 gtaactctgt tttgctgagt tcagggctgc agacagctgg tccacacggg aatcggcatt
     1021 attgtcagta tggctgccgg attcactgac tttgcgactg gtaaagtaat cgctggcctc
     1081 tttaaagtcc ttactggctc tagcgtcaat atcatggcga gcatcatggg aagccctgct
     1141 gccactactg gcttcatgtg agtccagatc actaccgtca atactggctc gcccaccaac
     1201 cttagcgcca acatcaacac gcacaccaaa aaggctgggc tttgcatcta atccggcatg
     1261 agcatcacca tacaatccag ctgatgcagt cgtacttctc gatgccagtt ctcttgtcgc
     1321 ctgctcatta ctgatattat gcgccttcgc atagctttcc actgcactgc gcatacggct
     1381 ggccatcatg gagtcctgtg cactcatcgt actgtcagcg ccaccggtga cagaatcact
     1441 gctcccccgg ttagaaccaa actgactgag cgtgttccag gcactggcaa tactgctgct
     1501 gaacccatgc agggcacttt ctgctctgtt cgatgcttcc cgggccattt cctgttgtgc
     1561 cgccgcaatc tgacgcgttg tattgatgcc gaccggtaac cgggacatcg ccccgctcgc
     1621 atccatcacc atattaccgt cacgggtctg tgtggcagtt gcgccactgc cggtctggta
     1681 catcatctga ccaaacgacg tggtgctgtt ggtgctccag ctgaagccgt tcacgttttc
     1741 cgtctgcatg ttgccgtagg agtaattacc gtcaaccaca ctacctgccg cactggcagt
     1801 cgggctgata gcagaggaag cgaaatggct gtacacactg gaaaatccgg ccccgagtcc
     1861 cctgaccatc atccaggata atggcgggat catcatggaa agatacccgg cagtggaggc
     1921 cagatcggag tatttcagct gtatctgtga aatttccgag agcaccaccg gtgcaccatt
     1981 ctgcttcgca taaaatgtca tggcactgtt cagaatggcg tacaacattg gccatgactg
     2041 cagccacatc agggcaaaca catagccttt caggacggac agcgtcagtt tattgaagac
     2101 agccgccagt accaggagcg ggaaaatacc aatggcgatc cccgtcagga tggtctgcgt
     2161 catgggcagt gtccgcatcg ctacatggcc gatggagaca tgtgccagac gttgcttctc
     2221 catcgatgag gtggtggcca gattcaccag gctggccgaa tcaccattac gagcggcata
     2281 actggtgatc ccctccttca gggcattgat ggtaacattc tggcggatga tctggctggc
     2341 tgatttactt gagccataga agtaactgta actgtcgcca atcagcgtac tgaacaacag
     2401 gttcggatca gggcgtccac caaatagctg ctgggcataa taatgccagg tttttccgcc
     2461 actctgtgtg tccagattca gcttgtcttt cagcgaaacc gacgcgtcct tacaggttac
     2521 gaaattatta ttgctgtcat aaacgccccg cagcgggctg gggcgggaaa aaatcagcgt
     2581 gtagggatca gcggaggcca tcagctcctc cagcgtgtat ttgtggttca ggtaaatatc
     2641 ccccatcacg cagttctgca cataatcctg gaaaatattg gcgatttccg gatgccggga
     2701 caaaaagtcg gtgctttttg ataccatttc tgcgccaaac agcatccccg ttttgctgta
     2761 ggtgacactg tccggttgcg tgaagatcat ctcgtaactg gccaccattg catgcccgat
     2821 gcgggtcgtc agtgaaagtg gcatcgccag accgaccggc acattatcca cccggtgaac
     2881 tttgaccagg tcactgttat caataatctg caccgatgtg cggacattaa ccagcaggct
     2941 gataagcaca aacacggcca cccagcccag caaatccatc acgttgtgcc gctgtaccca
     3001 cattacggcc accgcgagaa cagacaatgt gagcgcaatt ttttcaatgg aatcccacgt
     3061 cctggttccc ataaaggcgg caatggcgtt caggttattc cgcaaccact caccaccggc
     3121 aatcacataa acttcattca cagcgtgctc cctccgaagt gatagttgtt ctggtaacga
     3181 ctgagcatgc gggcggaaag ctgctgacgc atgtagctca tctgacgatc gacaaccagc
     3241 agcgcatcct gctgtacctg cacctgtgac tgaaacgccg caatctgccg ggtggcatca
     3301 ttcatgttgt cgttaatatg cccgataacc gcttcgtcat aatttcccgt ggccaccatc
     3361 gcccgggcct gctgtatcag ctcctgaatg tactgcagca ggatgtcgta accgatatag
     3421 tccgtcagct ggtaaatcat actgttggaa acaccgagca tctgcgggtc aaccaggtat
     3481 ttgaagacgg ggatggtggt actggaaata aagccttttt ccttgtcatc cagaggcgtg
     3541 tcactgacag ccttgttctg aatgctggcc agaagtttag taatctgaga cttcagtgca
     3601 ttatcccggc tgatggtcac cggtgtgtct gccaccactt tcaggcattt atcagaatcg
     3661 ttgcagtgat aaatttttgc tgtgccgcct tccatcatgg cccggataat tgagcggtca
     3721 gtggttctgg ctgacagcgg tgtgatttcg ccgttgggac caaacaccag tgagccggtc
     3781 agcgtcatca caaactcttt cagctctttg ttgccgtcaa acattctgtt tttggaaagc
     3841 gcattccaca tgatgttaat gtttttggtc acccgctcct tatccttgtc gctggcttta
     3901 tccctgacgc tgtcagattt accgccaacg gtacatccct g
//
LOCUS       DAJOWB010000241         3912 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJOWB010000241 DAJOWB010000000
VERSION     DAJOWB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.242"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            171..815
                     /locus_tag="OHO85_004772"
                     /pseudo
     CDS             171..815
                     /locus_tag="OHO85_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            815..1396
                     /locus_tag="OHO85_004773"
     CDS             815..1396
                     /locus_tag="OHO85_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCQ2674001.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTY
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPVIPEV"
     gene            complement(1516..2406)
                     /locus_tag="OHO85_004774"
     CDS             complement(1516..2406)
                     /locus_tag="OHO85_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HCQ2674002.1"
                     /translation="MFRHVKKLQYTVRVSEPNPGLANLLLEQFGGPQGELAAACRYFT
                     QGLSDEDAGRRDMLMDIATEELSHLEIIGSLVGMLNKGAKGALAEGTENEAELYRSLT
                     QNGNDSHITSLLYGGGPALTNSAGVPWTAAYVDTIGEVTADLRSNIAAEARAKIIYER
                     LINVTDDPGVKDTLAFLMTREAAHMLSFEKALHSIRNTFPPGKLPPIEKYKNVYYNMS
                     EGEDVRGSWNSDENFDYVSDPVPAVDGGDGKASINLSTKQEAMIKAMATRLKSHEDIN
                     PVTGAELAEGEPQTKINSKN"
     gene            complement(2425..2931)
                     /locus_tag="OHO85_004775"
     CDS             complement(2425..2931)
                     /locus_tag="OHO85_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCQ2674003.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAEKMLESMASRIENYPELRSRIEQ
                     HISETKNQLSQLESILDRNNISRSVIKDSMSKMAAFGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASVPIIEAILNEEKQMAEWLLNHIPDTTEQFMVRSE
                     IDGVEAKK"
     gene            complement(2968..3468)
                     /locus_tag="OHO85_004776"
     CDS             complement(2968..3468)
                     /locus_tag="OHO85_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCQ2674004.1"
                     /translation="MQIKSVEDIFIHLLSDTYSAEKQLTKALSKLSRSAYSDKLTAAF
                     QSHLDETHGQIERIDQVVDSEDGLKLKRIKCAAMEGLIEEANEVIESTDKNEVRDAAL
                     IAAAQKVEHYEIASYGTLVTLAEQLGYKKAAKLLKETLEEEKATDVKLTDLAFNNVNK
                     KAQDNS"
     gene            complement(3547..3729)
                     /locus_tag="OHO85_004777"
     CDS             complement(3547..3729)
                     /locus_tag="OHO85_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCQ2674005.1"
                     /translation="MTEHRGGAGNFAEDRQKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQNSHGGGRKSDS"
BASE COUNT         1047 a          865 c          848 g         1152 t
ORIGIN      
        1 acagctatat tctggttaac ttctataccg gagtcggaag ttgccggacc ttgcagatga
       61 aggcacatta cagaaatgga ggtctgttct accgttcctc aagagatggc tatggttttg
      121 aggaagactg ggcagaagtt tatacctcga aaaatcttcc accagaaagc tacccagtcg
      181 gtgcaccaat cccgtggcca tcagataccg ttccgtctgg ttatgccctg atgcaggggc
      241 agacttttga caaatctgca tacccgaaac ttgcagccgc ttatccgtca ggcgtgatcc
      301 ctgatatgcg tggctggacg attaagggca aacctgccag tggtcgtgcc gtattgtctc
      361 aggaacagga cggcattaaa tcgcacaccc acagcgccag cgcatccagt acggatttgg
      421 ggacgaaaac cacatcgtcg tttgattacg gcactaaatc cacgaataac accggggcgc
      481 atacgcacag tgtgagcggt acagccgcaa gtgccggaaa ccatactcat agtgtcacag
      541 gcgcatcagc agtcagccag tggtcacaaa atgggtcagt acataaggta gtgtctgcgg
      601 ccagtgtgaa tacaagtgct gcaggagcgc acactcatag tgtcagcggc acagccgcat
      661 ctgcaggtgc tcacgcacat actgtcggta ttggtgctca tacgcactct gttgcgattg
      721 gctcacatgg acacaccatc accgttaacg ctgctggtaa cgcggaaaac accgtcaaaa
      781 acatcgcatt taactacatt gtgaggcttg cataatggca ttcagaatga gtgaacaacc
      841 acggaccata aaaatttata atctgctggc cggaactaat gaatttattg gtgaaggtga
      901 cgcatatatt ccgcctcata caggtctgcc agcaaacagt acctatattg caccgccaga
      961 tattccggct ggcttcgtgg ctgttttcaa cagtgatgag tcatcgtggc atctcgttga
     1021 agatcatcgg ggtaaaacgg tttatgacgt ggcttccggc gacgcgttat ttatttctga
     1081 actcggctca ttaccggaaa atgttacctg gttatcgccg gaaggggagt ttcagaagtg
     1141 gaacggcaca gcctgggtga aagatgcaga agcagaaaaa ctgttccgga tccgggaggc
     1201 ggaagaaaca aaaaacagcc tgatgcaggt agccagtgag catattgcgc cacttcagga
     1261 tgctgtagat ctggaaatcg caacggagga agaaacctca ttgctggaag cctggaaaaa
     1321 gtatcgggtg ttgctgaacc gtgttgatac atcaactgca cctgatattg agtggcctgt
     1381 aatacctgaa gtttaaaata aaagcccgct taaaatatcg cgggcttcga aatataaatg
     1441 tattttcaga ggctagagct taggatatct cagcggcaat taacttatga gggatagcta
     1501 aataccagaa gataattagt ttttactatt tatctttgtt tgtggttctc cttcagcaag
     1561 ctcagcgcca gtgacaggat tgatgtcttc atgggatttc aaccttgttg ccatagcttt
     1621 tatcatagct tcttgcttgg tcgaaagatt tatcgaggcc ttcccatccc caccgtcaac
     1681 cgctggaact ggatcggaaa catagtcaaa gttttcatca ctgttccaac tacctctgac
     1741 gtcttcacct tctgacatgt tgtaatatac gtttttgtat ttctcaattg gtggtaattt
     1801 tcctggtgga aaggtgttac gaattgaatg taatgctttt tcaaatgaaa gcatgtgtgc
     1861 tgcttccctg gtcattaaaa atgccagagt gtcttttact ccaggatcat cagtaacatt
     1921 aatgagacgt tcgtaaatga tctttgcccg agcttcagct gcaatatttg aacgaagatc
     1981 ggccgtgact tcgccaatag tatcaacata agctgcagtc cagggtactc cagctgaatt
     2041 agttaatgct gggcctcctc cgtagaggag agaagttata tggctgtcat tgccattttg
     2101 agttaaggaa cggtaaagct cagcttcatt ctcagttcct tcagcaagtg ctccttttgc
     2161 acctttgttg agcataccaa caagagaacc aataatttcg agatgactta gttcttctgt
     2221 tgctatgtcc atcagcatat cccttcggcc tgcatcttca tcacttaagc cttgagtgaa
     2281 gtatcggcat gctgctgcaa gctcaccctg tggcccgccg aattgttcta aaagtagatt
     2341 agctaagcct gggtttggct cacttacacg tactgtatat tgaagttttt tcacgtgtct
     2401 aaacataatg cctcctcaaa agaattattt tttggcttca actccatcaa tttcagatct
     2461 gaccatgaat tgctcagtgg tgtcaggaat atggttaaga agccattcag ccatttgttt
     2521 ttcttcatta agaattgctt caataatggg tactgaagct gtatcacccg catttttggc
     2581 tgctgcgaga agagatgtgt aacacgcaat ttcaaattgt tcgaacacat agccactgat
     2641 cgaacctttg acaatttcat cagatggaaa aattccacca atggattgcc caaacgcggc
     2701 cattttactc atagagtctt taatgacaga gcgcgaaata ttattacgat caagtatgct
     2761 ttcaagctgt gaaagctggt tctttgtttc gctaatgtgc tgctcaattc tggaacgtaa
     2821 ctctggatag ttttctatcc ggctggccat agactcaagc attttttcag cttgtttctc
     2881 catcgcatgt gcatcacgaa gccagtcatg gtaatgttca attctattca taatctgttt
     2941 atcctaaaag aaattgaagt ctatagttca ggagttatcc tgagcttttt tattaacgtt
     3001 attaaatgcc agatctgtta atttgacatc agtagctttt tcctcttcca gtgtttcttt
     3061 caggagttta gccgcttttt tatatcctaa ttgctcagcg agtgttacca aagtgccata
     3121 actggcaatt tcatagtgct ctactttttg tgctgcagca atcaatgctg catcacgtac
     3181 ttcattttta tcagtgcttt caattacctc attcgcctct tcgataagtc cttccattgc
     3241 ggcacactta attcttttca atttaagacc atcttcagaa tcaacaacct gatcgatgcg
     3301 ttcaatttga ccatgtgtct catcaagatg tgactgaaaa gcagcggtta atttatcact
     3361 gtatgcagag cgactaagtt tagacagtgc ctttgttagt tgtttctcag cgctgtatgt
     3421 atccgataat aagtggatga agatatcttc aacagattta atttgcataa tcactccaga
     3481 gttttcactg cgagcccgta ggctcgcagt tagcttcagt tattcacgaa tgtaggggat
     3541 gatagattaa gagtcagatt tacggccgcc accatggcta ttctgaccac cttttttccc
     3601 tgcttctgat gctctttgcg gatcgttttt aaaattaccg ccactatgtt ggccgccttt
     3661 acgaccagcg tcagaagctt tttgtcgatc ttcagcgaaa ttacctgcac cgccacgatg
     3721 ttcggtcata tcatttctcc tctgattttc aaattgtaaa taacatgtgc cttgtcagca
     3781 cccaattaac tctagtaaca aataagaaag tgtcaaataa gagattggta tgcgcatctt
     3841 aatgtgagaa atagaaattg taaaaatgtg tactcagtga gtataatcta ttgatatctt
     3901 ggtgattaac gg
//
LOCUS       DAJOWB010000242         3887 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJOWB010000242 DAJOWB010000000
VERSION     DAJOWB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.243"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            362..592
                     /locus_tag="OHO85_004778"
     CDS             362..592
                     /locus_tag="OHO85_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCQ2674006.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLELEKADPDFMTEREDVVSDEGRVNL"
     gene            589..1005
                     /locus_tag="OHO85_004779"
     CDS             589..1005
                     /locus_tag="OHO85_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HCQ2674007.1"
                     /translation="MNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRAAVDATTEIKVALRLAGTPIGPN
                     DTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR"
     gene            1080..2645
                     /locus_tag="OHO85_004780"
     CDS             1080..2645
                     /locus_tag="OHO85_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCQ2674008.1"
                     /translation="MPLITRLMLQNFKKFPELDLRFSNDRNILVGDNESGKSTILLAL
                     DLVLSDSRHRVEALGVESLLSQSAVRHFQEGERRADQLPVLTADVFLSNGGEPDLNGR
                     QNLAGIDADGLRMRIAPMMEEYGQDIHHVLQQDPDNFPYEYYSVQFSTFSGGHFAGFR
                     RYLRHLFLDSARIDNEHAAQEYTRTVYSVNVPVADRYRLENSYRQQKMHFCARHLSAI
                     NDTLKTYQFGVRSGAKSGLEANLDITEDGISIRHRGKGRQCFIKTEFALQRHQQQGGE
                     IHALLLEEPENHLSHVSMKRLVNQLATERQTQVFIATHSSHISSRLDLRKAILLGATR
                     PVLMNELSAETAAFFMKAPDNNVLEFALARRVLLVEGDAEFILIEAFYHRLYGRAPED
                     DGVHIIAIGGTSFRRYLELARLLENRVAALRDNDGNYQQNCDERYADVLCSRSRVFAD
                     HDNSRSTFEICLYQDNADLCDALFRGTRRTLTVQDYMLANKAEAAFQLLQLHAEKLTV
                     PDYIQEALAWIRE"
     gene            2630..3652
                     /locus_tag="OHO85_004781"
     CDS             2630..3652
                     /locus_tag="OHO85_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HCQ2674009.1"
                     /translation="MDKRVIFAVAGSGKTTLLIRRLREDRRTLILTFTVNNEAHLRAQ
                     IIRRFGYIPYGIRVMTWFEFLHGFCFRPFLQEQLSSRGLSFNQPPSRIPRTNIRHYQD
                     PAGRLYHRRLAHLLTARGLLPDIRTRLARYYDELFVDEVQDFAGHDFNFLLELCRAEI
                     SVLCCGDFYQHTFDTSRDGNVNATLHEDITRYEARFRAAGIMVDCETLSRTWRCSATV
                     CEFITGQLNIRISAHGTHTTQIEIVTDEARSAALHADNTMIKLFYREHHRYGCHSMNW
                     GGSKGLDHFQDVCIVMGANHWMRLIQQKLAALPPSSRNRLYVACSRARGNIYFIPESH
                     LRRFRN"
BASE COUNT          947 a         1019 c         1038 g          883 t
ORIGIN      
        1 ggtcttctca tgttgatctt ccaacattgt tgatttaaca ggcattataa cttctgaaaa
       61 ccaaaaagat aataaaggca aagggataca agaattggct tatcaaatac tcggagatgg
      121 tgggctccga cttcaccaga aaccggatct gcacggggaa agagagaaaa taacataggc
      181 tgaacatatc catatgcctg acatctgcat attcatggta tacgcatatc ctcaccatct
      241 gcatatgctg aatctataca tatgcctaac atctgcctat actaaacata tgcacacacc
      301 cggtgcaatg aacatcattc cggatatgca tcacatatcc acacttaccc gggagagaat
      361 catgagaacc gtatccattt ttaaaaatgg caacaaccgt gctatccgcc ttccacgcga
      421 tctggatttt gagggggtga gcgagctgga gatcgtccgg gaaggggaca gcatcattct
      481 gcgccccgtc cggccgacct ggggctcatt cctggagcta gaaaaggccg atccggactt
      541 tatgacggag cgcgaggacg ttgtcagcga cgaaggacga gttaacctgt gaacaagatc
      601 tatatgctcg acacgaacat ctgctcgttc atcatgcgcg agcaacctga agcattgctt
      661 aagcacctgg agcagtcggt gctgcgcggt caccgtatcg tggtctcggc catcacctac
      721 tccgagatgc gcttcggtgc caccggtccg aaggcctcgc cacgccacgt gcaactggtt
      781 gacgcgttct gtgagaggct cgatgccgtc ctgccgtggg accgcgccgc ggtggatgcg
      841 accacggaga ttaaggtggc actgcgtctc gccggcacgc cgatcggtcc gaacgacacg
      901 gcgatagccg gacacgccat cgctgcctgt gccattctgg tgacgaataa tgtgagagag
      961 tttgagcggg taccgggtct tgtgctggaa gactgggtaa gataacgctg taaaacaaaa
     1021 caggcggtca ggcgcggccg gccgctcttt caggcagcgg tatataaagg aataaaaaaa
     1081 tgccactgat cacgcggctt atgctgcaaa acttcaaaaa gtttccggaa ctggacctgc
     1141 gcttcagcaa cgatcgtaac attctggttg gggacaatga gtccgggaaa agcactattc
     1201 tgctggctct tgaccttgtg ctgagtgaca gcaggcatcg ggttgaggcg ctgggggtgg
     1261 aatcgctcct gtcacagtca gcagtcaggc attttcagga aggtgagcgc cgtgccgatc
     1321 agttacccgt gctgaccgct gacgttttcc tgagtaatgg gggagagcct gacctgaacg
     1381 gcaggcaaaa tctggcgggt atcgatgcgg atggtctcag aatgcgcatt gctcccatga
     1441 tggaggaata cggtcaggat atacaccatg ttttgcagca ggatcctgat aattttccct
     1501 atgaatacta ttccgtgcag ttcagcacat tctccggtgg tcatttcgca ggcttcagac
     1561 gctatctgcg ccatctgttc ttagacagtg cacgcattga taacgagcat gccgcacagg
     1621 agtatacgcg cacagtttac agcgtcaatg tgccggtggc cgatcggtac cggcttgaaa
     1681 acagttaccg gcaacagaaa atgcacttct gcgcccggca tctgtccgcc ataaacgaca
     1741 cgctgaaaac gtatcagttt ggcgtgcgtt ccggcgctaa atcaggtctt gaggcgaacc
     1801 tggacattac tgaggacggt atttccattc gtcaccgcgg aaaaggaaga cagtgtttca
     1861 tcaaaacgga gtttgcgctg cagcggcatc agcagcaggg gggagagatc cacgcgctgt
     1921 tgcttgagga gccggagaat catctcagcc atgtcagtat gaagaggctg gttaatcagc
     1981 ttgccacgga acggcagacg caggtgttta ttgccacaca cagtagccat atctcctccc
     2041 gcctcgacct acgtaaggct attctcctcg gagctacccg gccggtactg atgaatgaac
     2101 tttccgctga gactgccgcc ttcttcatga aagcaccgga taataacgtg ctggaatttg
     2161 cgctggccag acgcgtgctg ctcgtggagg gggatgctga gtttattctg atcgaggcct
     2221 tttatcaccg cctgtatggc agggcaccgg aggatgacgg ggttcacatc atcgctatcg
     2281 gcggaacgag tttccgtcgt tatcttgaac tggcccgtct tctggaaaat cgtgtcgcgg
     2341 cccttcgcga caatgacggc aactatcagc aaaattgcga tgaacgctat gctgacgtgc
     2401 tttgttcccg ctcccgggta tttgctgacc acgacaacag ccgctccaca tttgaaatct
     2461 gcctgtatca ggataatgcc gacctgtgtg atgcgctttt ccgcggaacc cgccggacac
     2521 tcacggttca ggattacatg ctggccaata aggcagaagc agcattccag ctgttacagt
     2581 tgcacgccga aaagctgacc gttcctgatt atatccagga ggcactggca tggataagag
     2641 agtgatattt gcggttgccg gctcgggtaa aaccacgctg ctgatacgtc ggttaagaga
     2701 ggaccggcgg acgctgatcc tgaccttcac cgtaaataat gaagcacatc tgcgggcgca
     2761 gattatcaga cgctttggct acataccgta cgggatccgt gtgatgacct ggtttgagtt
     2821 tctgcacggt ttctgttttc gccctttcct gcaggagcaa ctgtcatcgc gtggtctgag
     2881 cttcaaccag cccccttcca gaataccgcg cacgaatatc cggcattatc aggatcccgc
     2941 cggacggctg tatcaccggc ggctcgcaca cctgctgaca gcccgggggt tgctgccaga
     3001 tatccgtacc cgacttgccc gttactacga tgagttgttc gttgatgaag tccaggactt
     3061 tgccggccat gactttaatt ttctgctgga gctatgccgc gcggaaatct cagtgctgtg
     3121 ctgcggggat ttttatcagc atacctttga cacaagccgc gacggtaacg tcaatgctac
     3181 gctgcatgaa gacataaccc gatacgaggc tcgcttcagg gctgcgggta tcatggtgga
     3241 ttgtgagacg ctcagccgga catggcgctg ttcagctaca gtgtgtgagt tcattaccgg
     3301 acaactgaac attcgcattt cggctcatgg aacacatacc acccagattg aaatcgtcac
     3361 ggatgaagcg cgttcagcgg ccctgcacgc ggacaacacg atgatcaagc tcttctatcg
     3421 cgaacatcac cgttacggat gccactcaat gaactggggg ggcagtaaag gactcgacca
     3481 ctttcaggac gtctgcatcg tgatgggggc caaccactgg atgcgcctca tccaacagaa
     3541 gctggcagca cttccaccat catcccgtaa caggctatac gtggcctgct cacgggcacg
     3601 gggcaacatc tattttattc cggaaagcca tcttcggaga ttcagaaact aagtcagcgg
     3661 cttctcaact gggcacaagc cggtaaaaag tagtcaacga caaaacttca ggccgaaaaa
     3721 tgaggctccg gtttcgccgg aaaccggacc tgcgtggagg gaaacccgca gatgatatga
     3781 tatttatcat taatcgcata ataaatcctg gcctgatatt tacaggcaga gaaataaatc
     3841 attcgatata tgattttatc gtcatcagcc attaaccccc caggaag
//
LOCUS       DAJOWB010000243         3879 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJOWB010000243 DAJOWB010000000
VERSION     DAJOWB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.244"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(27..1142)
                     /gene="mtnA"
                     /locus_tag="OHO85_004782"
     CDS             complement(27..1142)
                     /gene="mtnA"
                     /locus_tag="OHO85_004782"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HCQ2674010.1"
                     /translation="MNIKGKHYRTVWVSGDGKAVEIIDQTKLPFKFEVVALTSAEMTA
                     TAIQDMWVRGAPLIGVVAAYGIALGMNHDASDMGLQRYYDLLIKTRPTAINLKWALDR
                     MIDTLKDLCVSERKDVAWALAAEIAEEDVALCEQIGLHGAEVIREIAQKKPAGSVVNI
                     LTHCNAGWLATVDWGTALSPIYKAHENGIPVHVWVDETRPRNQGGLTAFELGSHGIPH
                     TLIADNAGGHLMQHGDVDLCIVGTDRTTARGDVCNKIGTYLKALAAHDNHVPFYVALP
                     SPTIDWTIEDGKSIPIEQRDGKEQSHVYGINPQGELSWVNTAPEGTRCGNYAFDVTPA
                     RYITGFITERGVCAASKSALADMFADLKSKALQGEQH"
     gene            complement(1152..2411)
                     /locus_tag="OHO85_004783"
     CDS             complement(1152..2411)
                     /locus_tag="OHO85_004783"
                     /EC_number="2.7.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose kinase"
                     /protein_id="HCQ2674011.1"
                     /translation="MTDSIPSGYKPLTCDTLPGYLSSRLTPSCEPGGLPEEWKVSEVG
                     DGNLNMVFIVEGTHKTIIVKQALPWLRAGGEGWPLSLSRAGFEYNVLCQEAKYAGHTL
                     IPQVYFYDPEMALFAMEYLTPHVILRKELINGKKFPKLAEDIGRFLAQTLFNTSDIGM
                     SAEQKKALTAEFALNHELCKITEDLIFTEPYYNAERNNWTSPELDDAVHKAWADVEMI
                     QVAMRYKYKFMTEAQALLHGDLHSGSIMVTDTDTKVIDPEFGFMGPMAFDIGNYIGNL
                     LLAYFSRPGWDANEQRRADYQEWLLQQIVQTWSVFTREFRQLWDNKTQGDAWSTEMYQ
                     QNRAALEDAQDQFFATLLEDSLVNAGMEMNRRIIGFAGVAELKQIENTELRAGCERRA
                     LTMARDLIVNARQFKNMDSVIQSAKVK"
     gene            complement(3151..3249)
                     /locus_tag="OHO85_004784"
                     /pseudo
     CDS             complement(3151..3249)
                     /locus_tag="OHO85_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
     gene            complement(3339..3707)
                     /locus_tag="OHO85_004785"
     CDS             complement(3339..3707)
                     /locus_tag="OHO85_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCQ2674012.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKK"
BASE COUNT          972 a          975 c          938 g          994 t
ORIGIN      
        1 gatccttaat agcaatggcc gctgttttaa tgctgttccc cctggagtgc tttactcttc
       61 aggtcagcaa acatatccgc cagcgctgac ttactggcag cacagactcc ccgctcagta
      121 atgaagccgg taatgtatcg ggcaggcgtg acgtcgaaag cgtagttccc acaacgggtt
      181 ccttcagggg cggtattgac ccagctcaat tctccctgag ggtttatccc atagacatgg
      241 gactgctctt ttccgtcacg ttgctcaatc ggaatgcttt ttccgtcctc gatagtccag
      301 tcaatagtgg gagaaggtaa cgcgacgtag aagggaacat ggttatcatg ggccgccagc
      361 gccttaagat aggtaccaat tttattacag acatcccctc tggccgtggt tcgatcggtc
      421 ccaacaatgc agagatcaac gtcaccgtgt tgcatcaggt ggccacccgc gttgtcggcg
      481 atcagggtgt gaggaatacc gtgtgagccc agttcaaatg ccgtgagtcc gccctgatta
      541 cgtggccgcg tttcatccac ccagacgtga acagggattc cgttttcatg cgctttgtag
      601 atgggagaaa gcgcggttcc ccagtctaca gtcgccagcc agcctgcgtt acaatgtgtc
      661 aggatattga ccacactccc ggccggtttt ttctgggcta tttcgcggat gacttctgca
      721 ccatgtaatc caatttgttc acacaacgcg acatcttcct cggcaatttc tgccgccagc
      781 gcccaggcca catccttgcg ctctgataca cagagatcct tcagtgtgtc tatcatcctg
      841 tcgagggccc atttgagatt aattgccgtt ggtcgggttt tgataagaag gtcatagtaa
      901 cgctgcagac ccatgtcact ggcatcgtga ttcattccca gcgcgatgcc ataggctgca
      961 acgacaccaa taagcggagc gccacgaacc cacatatcct gaatagccgt agcggtcatt
     1021 tccgcagagg taagcgccac cacctcgaac ttaaaaggca acttagtctg atcgatgatt
     1081 tctaccgctt ttccatcccc ggaaacccag accgtacgat agtgtttacc tttaatattc
     1141 ataatgacct cttacttaac cttcgcagac tggatgacgg aatccatatt tttaaactgg
     1201 cgggcattga cgataagatc gcgcgccatg gtcaatgcac gtcgttcaca tcctgcgcga
     1261 agctctgtat tttcaatctg tttcagctcg gcaacgccag caaaaccaat gatccggcga
     1321 ttcatttcca tgccggcatt caccagggaa tcttcaagca gcgtggcaaa aaactgatcc
     1381 tgtgcgtcct caagagcggc cctgttctgt tgatacattt ctgtcgacca cgcgtcgccc
     1441 tgtgttttgt tgtcccagag ctggcggaac tcccgggtga aaacggacca ggtttggaca
     1501 atctgctgaa gcagccattc ctgatagtca gcgcgacgtt gctcattcgc atcccaccca
     1561 gggcgtgaga agtacgccag caggaggttg ccaatatagt tgccgatatc aaacgccatt
     1621 ggccccatga aaccgaactc cggatcaatc actttggtat ccgtgtcggt caccatgatt
     1681 gagcctgaat gaaggtcgcc atgtaacaat gcctgcgctt ctgtcataaa tttgtactta
     1741 taacgcatgg caacctggat catctctaca tcagcccagg ccttatggac ggcatcgtcc
     1801 agctcaggag aagtccagtt attccgttca gcgttgtaat agggctctgt gaagatcaga
     1861 tcttccgtaa ttttgcacag ctcatgattc aacgcgaact cggcagtaag cgcttttttc
     1921 tgttctgctg acatgccaat gtcagacgta ttgaagagag tctgtgctaa aaatctgccg
     1981 atatcttcag ctagtttagg gaatttttta ccgttaatca gttccttacg cagaatcacg
     2041 tgaggagtca gatactccat ggcaaacagc gccatttccg ggtcgtaaaa atagacctgc
     2101 ggaatcagtg tgtgacccgc gtacttggct tcctgacata agacgttgta ctcaaagccc
     2161 gcacggctca gagataaagg ccatccttcg cccccggcac gaagccaggg cagagcttgt
     2221 tttacaatga tggttttatg tgtcccctca acgataaaca ccatgttcag gtttccgtcc
     2281 cccacttctg aaactttcca ctcttcaggt aaccctcccg gttcgcatga aggggtcagt
     2341 ctggacgaga gataacccgg cagcgtgtca catgtcagcg gcttgtaacc tgatggaatt
     2401 gaatccgtca taacagttct ccttgtctac acagaatgat aatcaactta tcatgtgaca
     2461 actatacatt atcaaatgta aaattatttg tgacacatgt agattttttg aactgtataa
     2521 aattaaaaat gatgtcgtat ggagtcatga tccttacagg aatcagcaga ggaaaacatc
     2581 tattattacc atgtaattca tgttgttatg tcgcaacaat agtactgcac ggggaattat
     2641 tacgggcaaa cttaggcgaa aggaagtact gaagtgacag aataaatcac actttttgta
     2701 catatgtact tttctattgt cttttgttgt ggaaacaaat ggtgtttgtc atgccgatgc
     2761 taatagacag atagattcga gggccaacag ccccatcatc atgatggagc aacagaggtg
     2821 agtctaacgt ttccgcactt cttatcgatc ggcactaacg atcaatatcg ataaactgat
     2881 ctatcaaaac gattatcttt cttgcaacct gcctagttat ccttctctta cattgctcgc
     2941 agaaaagccc ttttattacg gttagttatc tagctgaggc ttgctacgcc cattttccat
     3001 gtaccccaaa tcacgaggtc acaaaggcgc ggaaagatga agggatgtgc acgttgcaac
     3061 aaaaaaaaga agcgtccttg aacagaggct ctcactcgat aaagaattct gcgacgagtg
     3121 cttattcgat atcgatgagg aaatcaggga atgctggtcc agtaaccggg tgagcagtgt
     3181 ctgccagatg gtgactggag acgggcagca cgatacctcc cgtacatgcg tgtcgggtaa
     3241 tgttttcata ctgaagattt tcctgatatg cagagataaa aaatgggaca gtggcgtggt
     3301 gaaaacacca ggccggagca gaaggttatt ctggtggttt actttttcgt ttcaggtgtc
     3361 ggataaacag ccagataaac gtaaccacag gagccgaggg tgtcggcttc acaggtcagc
     3421 cccttcgcat acagtgtcac ggtatgctga tggcggggat tcagttcacc actggtgagc
     3481 attaatttca gttgtttcat cagcagcgga aaggcctggt ccagagggta cgcatccgca
     3541 tctctgaacc ggcctctgat accggcacgg tcggcaaggt aatgcaaccg gttaccctcc
     3601 tgcaccagac gggcgccgaa acagggcgtc actgtgcagg gtagtcccca ccagggatgg
     3661 tcgtcattgt catcgggatg cgttgtcccg gaaagtgtgt ctgacacgat aaaatcctca
     3721 cagaaaatcg gtgaatgata gtttaacgat gacgggtcag ccggcgcgca gaccggcttc
     3781 ggtaaaggtg tattcgttga tgagcaggac ctcatccaca gcggcgtctg agctctgcca
     3841 gtcgtactcg ttttccgggt gacggaacat cagttcagg
//
LOCUS       DAJOWB010000244         3823 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJOWB010000244 DAJOWB010000000
VERSION     DAJOWB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.245"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            84..254
                     /locus_tag="OHO85_004786"
     CDS             84..254
                     /locus_tag="OHO85_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674013.1"
                     /translation="MLEQNLTVEIDNYGSFKPTISLDDFPRGLSEREAMLKLAEWLQR
                     LSIAIEDNWSEP"
     gene            261..635
                     /locus_tag="OHO85_004787"
     CDS             261..635
                     /locus_tag="OHO85_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674014.1"
                     /translation="MMTLKHFLDRPLWAAAAGYDFNYMDCMSYTANAYDYSFSLLLNS
                     LRILPQTEVGELHLWLLGFIAAGVGIAVWPFIFWLVAVVVWFKCKTYRRKYFLGDGMT
                     DIAKMNIEKWTKECEKKWRKKK"
     gene            617..1555
                     /locus_tag="OHO85_004788"
     CDS             617..1555
                     /locus_tag="OHO85_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674015.1"
                     /translation="MAQKEMTRITEERISEIISRIEMYGHGAGYTADEVLALAQMALA
                     ACKDDKKMKFIDLLVKELPKCGGWPDGMSYCYLPSVNLMAPCATFAFGSDHKKDTFFG
                     RNFCCEIELPIGDLDSDEYQSVVTREQYESALIASQKVEFNGDELESKTYRLDFGQWL
                     EQQRGKIDVDCGCVSTETFMHWLRVAYEAGNYPDIPDSSVPAPGKGVTGERIRIKPHV
                     YRELVNRLHDTAIKCAGTQQLRERISRVLGDVITPDHHKQAEKSGLERCHLEAALNIK
                     PGHTLGIIDALLVHKMARALLPLVAEKHEVDHANES"
     gene            1539..1823
                     /locus_tag="OHO85_004789"
     CDS             1539..1823
                     /locus_tag="OHO85_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin PHD"
                     /protein_id="HCQ2674016.1"
                     /translation="MPTKAELQVRVDELEKENASLKKMLSRAERELSGKLLPEELPPA
                     DIPDRVSWWMKYFRAPWEAFWCYDHRRWCDELDSNFPYFAEGNTCPQCRG"
     gene            complement(1808..2017)
                     /locus_tag="OHO85_004790"
     CDS             complement(1808..2017)
                     /locus_tag="OHO85_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674017.1"
                     /translation="MRFILTLKSETEGIKHSVKVFSEAILLNGKLRHLVRPERQYPDV
                     LAREKDLLSEVQDRVIDFVKCYPLH"
     gene            2191..2442
                     /locus_tag="OHO85_004791"
     CDS             2191..2442
                     /locus_tag="OHO85_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCQ2674018.1"
                     /translation="MSDRNIAAKSQEERDKVNVDLAASGVAYKERLNIPVIAEQVARE
                     QPENLRAYFMERLRHYRQLSLQLPKGSDPVYQNEDAPKK"
     gene            2566..2955
                     /locus_tag="OHO85_004792"
     CDS             2566..2955
                     /locus_tag="OHO85_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HCQ2674019.1"
                     /translation="MGFPSPAADYVESRISLDQQIIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCAMEGEYRIKRYRKYPRQHLEDLSTGKKEALPVDDDGYT
                     GSNAVFGVITHVINDARSGEFDDCPVI"
     gene            3028..3249
                     /locus_tag="OHO85_004793"
     CDS             3028..3249
                     /locus_tag="OHO85_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001603498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCQ2674020.1"
                     /translation="MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATF
                     EAYKKAALDAEFASLFDTLDSTNKELVNR"
     gene            3249..3629
                     /locus_tag="OHO85_004794"
     CDS             3249..3629
                     /locus_tag="OHO85_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCQ2674021.1"
                     /translation="MRHISPEELVALHDANISRYGGLPGMSDPGRAEAIIGRVQARVA
                     YEEITDLFEVSATYLVATARGHIFNDANKRTALNSALLFLRRNGVQVFDSPELADLTV
                     GAATGEISVSSVADTLRRLYGSAE"
     gene            3634..3813
                     /locus_tag="OHO85_004795"
     CDS             3634..3813
                     /locus_tag="OHO85_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PdcA protein"
                     /protein_id="HCQ2674022.1"
                     /translation="MARKYNKLSRESLKMLLDGVSRRKVKQYLVGKQIGVRTAIAVLC
                     RQEMVVLKQRMPGSR"
BASE COUNT         1046 a          808 c          987 g          982 t
ORIGIN      
        1 ttaccgaata tcagtttgac cataaaacca ataaatcacg ttcagtatat ttgcttcggc
       61 acaatagccg cgtaaggaat accgtgctgg agcaaaatct gactgttgaa atagataatt
      121 acgggagctt caagccaaca atttcgcttg atgattttcc tcgtggttta agcgaaagag
      181 aagcaatgct gaaattagca gaatggctac aaagattaag cattgctatt gaagataact
      241 ggtctgaacc ttaaatttat atgatgacac taaaacattt tcttgaccgc ccattatggg
      301 cggcagccgc aggctatgac tttaattata tggattgcat gtcttatacc gccaatgcat
      361 acgactattc cttcagtctg ctgcttaatt ctttaagaat attgccgcaa acagaagttg
      421 gagagcttca tttatggcta ttgggcttta tcgcggctgg agttggtatt gctgtatggc
      481 cttttatttt ctggctggtg gctgttgtag tgtggtttaa gtgcaagacg taccggagaa
      541 agtatttctt aggtgatgga atgactgata ttgccaaaat gaacattgaa aaatggacta
      601 aggaatgtga aaagaaatgg cgcaaaaaga aatgaccaga atcactgaag agcgtatatc
      661 agagattatt tcccgtatcg aaatgtatgg tcacggtgct ggatatacgg cagatgaagt
      721 attggcactt gcccaaatgg ctttggcggc ttgcaaagat gataaaaaaa tgaagtttat
      781 cgacttgtta gtgaaggaac tgcctaagtg cggcgggtgg cctgatggaa tgagttattg
      841 ttacctaccc agtgtcaatt taatggcacc atgcgcgact tttgcttttg gctcagacca
      901 caaaaaagac actttctttg ggcgcaattt ttgttgtgag attgagcttc caattggtga
      961 ccttgatagc gatgaatacc agtcagttgt cactcgcgaa caatacgaat cagctctcat
     1021 agcgtcgcag aaagtcgagt tcaatggtga tgaacttgaa agtaagactt acaggttgga
     1081 ttttgggcaa tggctggaac agcaacgcgg gaaaatcgat gtggactgtg gttgtgtgtc
     1141 cactgaaaca ttcatgcact ggctgcgggt agcttacgag gctggcaact atccggatat
     1201 tccggatagt tcggtgccag caccaggaaa gggcgtcacc ggtgagcgta tccgaattaa
     1261 gccgcatgtt tatcgcgaac tggttaaccg tctccacgat acagcgatca agtgtgctgg
     1321 cacccagcaa ttacgagaaa gaattagccg tgttttgggc gacgttatta caccagatca
     1381 tcataaacaa gccgagaaaa gtggcctgga aaggtgtcac cttgaggcgg cattaaacat
     1441 taagccgggg catacgcttg gcattattga tgcactattg gttcataaga tggccagggc
     1501 tttattgccg ctggtggctg aaaagcatga ggtggaccat gccaacgaaa gctgaattac
     1561 aggtgcgcgt agatgagctt gaaaaagaga acgcgagcct caaaaaaatg ctgtcgcggg
     1621 cggaaaggga attatcaggc aaattattgc cagaagaact gccaccagca gatatacctg
     1681 atcgagtgtc ctggtggatg aagtatttcc gtgcaccgtg ggaggcgttt tggtgctacg
     1741 accatcgcag atggtgtgat gaacttgata gcaattttcc ctactttgcg gaagggaaca
     1801 cctgccctca atgcagggga tagcatttga cgaaatcgat caccctatcc tgaacttcag
     1861 aaagaaggtc tttttcacgc gccaggacgt caggatattg acgttctggc cttaccagat
     1921 gccggagctt cccattaagc agaatggcct cagaaaacac ttttacgctg tgctttatcc
     1981 cctctgtttc acttttcagc gtcaaaataa agcgcaattg cttattagcc tgattcggat
     2041 tcaatacacg aatttgcagt tcgtctatgc tgttccgcaa tgggattgat gccaccacac
     2101 tggtgcagtc tctgattgtc tgaattgaac ggctaacatt gagaacgtta ttgtgcatgt
     2161 gcctgatcca ctaactcctg gaggtttctt gtgtcagatc gaaatatagc agctaaaagc
     2221 caggaagagc gagacaaggt gaacgtagac cttgccgcca gcggcgttgc ttacaaagaa
     2281 cggctgaata tacctgtgat tgcagagcag gtggcccgtg agcaaccgga aaacctgcgc
     2341 gcctatttca tggaacggct acggcactac cggcagttaa gcctccagtt gccaaaaggg
     2401 agcgatccgg tgtatcagaa cgaggatgca ccaaaaaaat aacggcaaga tgggggagaa
     2461 atgtgattag cccccagcgt ggcgcgccta caaaccccgc tttcacaaac tatgcctttt
     2521 caatgtatac tgtatgaata aacagtatca ttgaggtaaa acgctatggg cttcccttct
     2581 cctgcggcgg attatgttga aagccgaatt tctcttgatc agcagataat tagacatcct
     2641 tcagcgacct acttcatgcg ggcagctgat agccatcacc gtgagggaat attgcagggt
     2701 gctttgctgg tggttgattc ctcgcttact ccggttgatg gttctctgct tgtgtgcgct
     2761 atggagggtg aatatcgcat aaagagatac aggaagtatc cgcgccagca cctggaggac
     2821 ttaagcaccg ggaagaaaga ggcgttacca gtagatgacg atggatacac gggcagtaat
     2881 gctgtttttg gtgtgatcac tcatgtcatc aatgatgccc gaagtgggga atttgatgat
     2941 tgtccggtta tttaagctgc aaagtgctgg tgctttatgc ctgtgaagtt tataattgtg
     3001 tacacataac gagtacacga ggtgtttatg caatccatta acttccgtac cgcgcgtggc
     3061 aacctttctg aagtgctcaa caatgttgaa gccggggaag aggttgaaat cacccgcaga
     3121 ggccgtgagc cagcagtaat tgtcagcaag gctactttcg aagcctacaa aaaagcggcg
     3181 ctggatgctg aatttgcatc cctgtttgac accctggact ccaccaacaa ggaactggtt
     3241 aaccgataat gagacatata tcaccggaag aacttgttgc gcttcatgat gcgaatataa
     3301 gccgctacgg cggcctgcct ggcatgtcag atccgggtag ggcagaggcc attatcggga
     3361 gagttcaggc cagagttgcc tacgaagaga tcaccgacct tttcgaagtc tccgccacct
     3421 acctggtggc tacagcgaga gggcatatat tcaatgatgc caataagcgt accgcgctaa
     3481 acagtgcgct gctatttcta cgccgtaacg gggtgcaggt atttgattca cctgaactgg
     3541 cagaccttac tgtaggcgct gcgactggcg agatatctgt atcttctgtc gccgacacgt
     3601 tacgtagatt gtatggttct gcggagtaga ttaatggcac gcaaatacaa caaattgtcc
     3661 cgtgaatcgt taaagatgct tcttgatggc gtgagtcgcc gcaaggtaaa gcaatacctg
     3721 gttggtaagc aaattggagt caggaccgct attgctgtgt tatgccgtca ggaaatggtt
     3781 gtgcttaaac agagaatgcc gggcagcaga taaagcccaa tca
//
LOCUS       DAJOWB010000245         3685 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJOWB010000245 DAJOWB010000000
VERSION     DAJOWB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.246"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..3120)
                     /locus_tag="OHO85_004796"
     CDS             complement(<1..3120)
                     /locus_tag="OHO85_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCQ2674023.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEGNTNIVGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPAKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVSADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIRPGHNYYFYVRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNEVFLKYLTAPTITSGGNP
                     PAFSLTPDGKLTAKNADISGSVNANSGTLNNVTINENCQIKGKLSANQIEGDIVKTVG
                     KAFPRDSRAP"
     gene            complement(3181..3681)
                     /locus_tag="OHO85_004797"
     CDS             complement(3181..3681)
                     /locus_tag="OHO85_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCQ2674024.1"
                     /translation="MPAFRQKLSDGWYQVRIAGRDTGETELSARLNEPLANGAVIHIV
                     PRLAGAKSGGVFQAVLGAAVMAVAIWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKP
                     KTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQ
                     VVVIGR"
BASE COUNT          739 a         1167 c          926 g          853 t
ORIGIN      
        1 cggtgcccgg gagtcccgcg ggaaagcctt acccactgtt ttgacaatat cgccttcaat
       61 ctggttggct gacagtttcc ctttaatctg acagttctca tttatcgtaa cgttgttgag
      121 cgtcccggag ttcgcattca cactgccact gatatctgca tttttagcgg tcagctttcc
      181 gtccggtgtc agggaaaatg caggaggatt accgccgctg gtaatggtgg gagccgtcag
      241 atatttcagg aacacttcat tcatgaatat ctgatcaccc tgaccaacaa acatcggttt
      301 tgtgttgcca tttgcaggat taatcatcgc aatcctgtct gccgccagca gcacctgact
      361 ctgcatgccg tcgggggtgt tctcaatacc ggcaccgata cccgcgatat aaaggcgtcc
      421 gtcctgcatc tgctgcagct tcacagccca catgctgttc agattattat ttgtatcaac
      481 ctgaaccttc tgtatctgct ggatcgctgc actctggtct tccagtttct tattgacggt
      541 ctgtgtgatt tcattgctga catccgttat ggacgtcctg atttcagcca ggtcaggcgc
      601 aagctgaccg ttatcaatct gcgtccacag ctcctgagcc agatgggttt tccctatctc
      661 gcctttgaaa aaatccagat agccggatgc gtcatcactc ggctgaccaa cagcctccac
      721 gaatgccgat ttgccaacgg tgttcacact gcgaacgtaa aaataataat tatggcccgg
      781 cctgatattg atactggcgg ctatccagta cagcgccgtg ccaagatagc gtgctgtggt
      841 ttcaacctgc ctgatatccg caatccgttt ttccgagaac cagaactcaa actgtaccgt
      901 cggatcataa accgcaagat gcggcgtggc ggttatctga aaatagcccg gcgtcagctc
      961 aatccgcgac ggcgctgccg gtgcggcaat ccggaacgat accgacgccg gatcgccctg
     1021 ctgcccccac gcatttactg cccggactgt cagcctgtag ttccccagag ccagttgtgt
     1081 gaagcggtaa gtggtttccg tcgtccgggc cgtgctgacc agccgctcac tgccgtcatc
     1141 cgctgccacg gtcaggcgaa gcatgaaact cacgcccttc accaccttcg gcgtgtccca
     1201 gcgggccagt acctgatatt ccccgctgtc tgcggagact tcggcggtca gatgctgcac
     1261 tgctggcggc gtgacaccat tcaccgtgcc gctctggtcg ccgtcaaagt gcgccccgtt
     1321 atccacgatg gcctcttttg ccggtacatg ctgcacggcg gtgatggcat acgtgccgtc
     1381 gtcattctca cggatactca cacagcggaa caggcgctgg cgcagcgtcg gcagcttcag
     1441 cccccacacg ctgtatccgg caacgccgtc aggaacacgg ctcactttta ccttcacgcc
     1501 gtcggtgacg gactggacct ccacgctgac cggattgcca cttccgtcaa ccaggcttat
     1561 cagcgtggta ccggaggatg gcagcgtgat ttcacggtcg agcgtcagcg tccgggtctg
     1621 gctgttcacc gccagcacac gcccaccgat gctgatcccc gcatagtcat catcacagat
     1681 ttcaatgaca tcgcccggta catggcgaag cccttctgcg cccacgctga agtccacggt
     1741 ctgcgtctcc agcagctccg ttttaatcag ccacagcccg gcgcggtgtg cctgcccccg
     1801 gctggtacag ccaaaggcat ccattttcgt gacgttacga ccgtaacggg caatggcctg
     1861 cgtatcctcc acaagctctg tcgccgtctc ccagccgtta ttcgggtcaa tccagttcac
     1921 ctcaacggca ttatggcggt ccttcagggc gctgaagctg tagcggaacg gcgcaccatc
     1981 atccggcatc accacattac tgcggttata ggtccacacc ttatctgatg gccggtcctg
     2041 cacgaacgtc agcgtctgcc cgttccatac cggcatacag cgcatcgccg agcagaaatc
     2101 actgagcaca tcccacgcct tgcgctgtgt ggtcaggtac gcattacagg tgatgcgcgg
     2161 ctccgtgccg ccaaagccgt ccggcactga ctggtcgcag tactggccga tgacatacag
     2221 cgcccattta tccacatccg ccgcaccaag acgtttcccc atgccgtagc gcggatgggt
     2281 cagcatatcc cacagacacc aggccatgtt gttgctgtat gccggtttaa acgttccgtc
     2341 ccagataccg ctgtattgcc gcgtctgcgg gttataattc gacggcacct gcagaatacg
     2401 cccgcgcaga tgataattac ggctcacctg ctggctgccg aactgctccg agtccacctg
     2461 cacgccgacc agtgccgtgt tcgggtagca ctgtttcaca tcgatgattt cggtgtatga
     2521 cgaccagagc gttttgttct gcagctggtc tgtggtgctg tccggcgtca tcctgcgcat
     2581 ccgtatattg aacgggcgcg gcggcaggtt acccaccacc accgaggcca gatactgcga
     2641 ggtggttttg cccttaatgg tgatgtcttt ttccgtcacc cagccaccgt tacgttgtat
     2701 ctgaaccagc aggcgaactt ccgacggatt ccggtccccc tttgaggtgg tttccaccag
     2761 tgcctgcaca ccgaaggtaa agcgcagtcg gtcgatgttt gccgacgtga tggtgcgggt
     2821 gatcggcgtg tcgtatttca cttccgtacc cagcaccgtc tcggagccgg aggattcaaa
     2881 tccctccggc ggagtctgct cctgctcacc ggcccggaac accaccgtga caccgacgat
     2941 gttggtattc ccctcactgt ccagcaccgg cgtactgttc agcagcacgc tttttaatcc
     3001 atccaccgga ccttcaatcg gcccttcact gatggcatca atcacactca gcaactgcgt
     3061 ggatttcagg ttgtccttcg cttcgcgcgg ggtatgcccc ttactgctgc ctttacccat
     3121 tcatcacgct ccataaacga caaaaccgcc cgcaggcggt ttcacataaa atgttttgca
     3181 tcagcgacca atcaccacaa cctgaccacc atccccttcg tctgccgtgc tgatctcctg
     3241 agagaccacc cgcgacccca cgcgcatttc accgtacaga acgggcagaa cattgccctg
     3301 ggcaaccatg ttatccagtg aggagaaata ggtgttctgt ttgccgttat ccgttgtctg
     3361 tgtgcggggg gttttgggtt taggggccag catctgtgca acaccgccaa gcgtcatact
     3421 ggcaccgaga gaaaacagca gattactcgc cataattcct acccccggca tccatatagc
     3481 aaccgccata acagccgccc ccagcacagc ctgaaacaca ccgccacttt tggctcctgc
     3541 cagacgcggc acgatatgga tcacagcacc atttgccagc ggttcattaa gacgggctga
     3601 taattccgtt tcacctgtat cacgcccggc aatgcgtacc tgataccagc cgtcgctcag
     3661 cttctgacga aacgccggga tctgc
//
LOCUS       DAJOWB010000246         3609 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJOWB010000246 DAJOWB010000000
VERSION     DAJOWB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.247"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(363..686)
                     /locus_tag="OHO85_004798"
     CDS             complement(363..686)
                     /locus_tag="OHO85_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCQ2674025.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            970..1164
                     /locus_tag="OHO85_004799"
     CDS             970..1164
                     /locus_tag="OHO85_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCQ2674026.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEL
                     AEELQNSIQTRFEAESGTGV"
     gene            complement(2023..3540)
                     /locus_tag="OHO85_004800"
     CDS             complement(2023..3540)
                     /locus_tag="OHO85_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017899153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HCQ2674027.1"
                     /translation="MKKNIVRQVLESYGPCISSELAERIKRQNPSMSSDAIRKMISRS
                     TDIGKLPFLRFSHNRRFIYLKDDFGSFKFWRALKKCMREANSAYSHAILSVINNGGYL
                     KVKDFGIVSGSPIKQAKHLSYESVLKNLLSAKILRSVYIDGIGDCVLINNNIANDISI
                     FNMANCESFFDKPIFELIKAWLRNLGIVAFNQIKTKYDGENNPVVGSFEWDITAPSYV
                     SPLAEYSSDGLIPGFVVCDFALGFNRNEITTDAADTFIRKVQMTKSSRTNQRIMFVLF
                     ARRFEKNAFNKLRSEGVLAITIANAFGNKVDESLARLSKVIQGTLSIERHPDELLQMV
                     KDLESISGENGNLRGYIFELFVSSQICNFYGYGNVRINKEYKINGKHAEADVVLETND
                     DIYIVECKNVKTLPSMEVSLWMKTRVPIINSYYKSNNPDNKNIHHRLWVTGNIYPKDI
                     NRLDEFKCNNKKIDVDYLFGQSLDDFFYQKKQVFNLYRKVISPDYKKGQIPDFDL"
BASE COUNT         1090 a          868 c          692 g          959 t
ORIGIN      
        1 aaaaaaaaac acaaagaaaa aaatcataaa aatcaaacat ttaacaaaag caacatatca
       61 caaaaaacac acctgcgaga tttatcctta aaaatcaaga tgttacgttt gtgacatttc
      121 atcatcatat atcaaccgcc ctatttacca taatccctta attgtacgca ccactgaaac
      181 gcgctgaacg cgattctgac gcagacaccg caaaaagaga tgttttaccc cggaaaaata
      241 ggaaggatgc gtcaggatcg ttttcagctc gctgcatagt tatgcatgaa agcgaatggt
      301 gatcactttg ggagcttacg gtgttcatac cgtctgtttt caacagtttt ctccccggga
      361 agctaatctg ccataagcct ggataacagg gcacggtgat actccgtaat agcgatcagc
      421 acctccgcat actccctgtc cccgatacgc ttgccgtcga taaagagttt tttctgtaac
      481 gcccccagtc tcataacttc tttcagtacc cgatcatcag tcagtgagtt aactttctta
      541 ccgagagctg ccgcccgtaa aaaaccggaa acggtcttgc cgcattcagc agcccttttt
      601 ctgatttcct ggtcttcttc tgccgtcaga cgaacagggc gactgatagc ccgcctgcgc
      661 gtattacttc cacttcgttt tgtcataaaa catggctccg tatctgacat gggtgtcggg
      721 gcaaagccct gaccagggta attgtaatag cgtgcatgta tgcgcggtat aacaattgca
      781 catcctgtcc tgtcagcaag ctcgaattac cgacaaaggc acggcagttt tttggcagtg
      841 acagtgttgt ctgacagaaa tgcccgtcaa aaagccaggc ggcagaatag cggcagcaaa
      901 acagtcgaaa agtggtacgc gatataatca cataacgcag atgacagaca gaacggagaa
      961 aacgaaaaga tgaacaagca gcagcaaacc gtactcaaca tggcgggatt cataaaaagc
     1021 cagagcctga cgctgctcga aaagctggac gcactcgatg ccgacgaaca ggccgccatg
     1081 tgtgagaagc tgcacgaact cgcggaagaa ctccagaaca gcatacagac acgctttgaa
     1141 gcggaaagtg gcaccggggt ataacagtgt atcctgctcc agacaggatg cagaacggta
     1201 taccggtgcg taaggagaaa atgccacata aggcgctctt ccgcttcctc gctcactgac
     1261 tcgctacgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa gacggtaatg
     1321 cggttatcca cagaatcagg ggataaagcc aaaagaaaca tgtgagcaaa aaacaagaac
     1381 ccgaaaaagg ccgcgccggt ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1441 aaaaatcgac gctcaagcca gaggtggcga aacccgacag gactataaag ataccaggcg
     1501 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgtt taccggatac
     1561 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgttggtat
     1621 ctcggcccgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1681 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccgaa aagacacgac
     1741 ttatcgccac tggcagcagc cactggtaac tggaatagca gagcgatgat aatgcggtcc
     1801 gtactacaga gttcttgaag tgatgcccca actacggcta cacttaaagg acaggatttg
     1861 gtatctgcgc tctgctgaag ccagttacct aggttaggta atctgccagg attaactaac
     1921 cttcgaaaaa ccaccttgcc gggtggtttt ttcgtttcat ggaggaagat acgcttttca
     1981 agccgtcatc gtcaagaaat gcagagacta gaaaacgtca ttctataagt caaaatcggg
     2041 tatttgccct tttttataat ctggcgaaat gactttccta tataaattaa acacttgttt
     2101 tttttggtag aaaaaatcat ccaaagactg accaaataaa taatcaacat caatcttctt
     2161 attattacat ttaaactcgt ccaatcggtt tatatcttta ggatatatat tccccgtaac
     2221 ccacaaacga tgatgtatat ttttgttatc agggttattt gatttgtagt aggaatttat
     2281 aattggcact cttgttttca tccaaagact aacctccatc gaaggtaaag tcttaacatt
     2341 tttgcactca acgatataaa tatcatcgtt agtttccaat acaacatcag cttcggcatg
     2401 tttaccgttt attttatatt ctttattaat acgaacattt ccataaccat agaaattaca
     2461 aatttgagag gaaacaaaaa gctcaaaaat ataccccctt aaatttccat tctctccaga
     2521 gatagactcc agatctttta ccatttgaag taactcatca ggatgtcttt caatagataa
     2581 agttccctga atcaccttcg atagtctggc caagctctca tcgaccttat taccaaacgc
     2641 attagctata gttatagcca gaaccccttc actccttagc ttgttgaagg cattcttctc
     2701 aaaacgtcta gcaaaaagaa caaacataat cctctggttt gtcctagagg acttggtcat
     2761 ctgaactttt cttatgaagg tatcagcagc atcggtggta atttcatttc gattaaaccc
     2821 tagagcaaaa tcacagacaa caaaacctgg gatgagtcca tcgctggaat attcagcaag
     2881 aggggacaca taacttggcg ctgttatatc ccactcgaaa gaaccaacca caggattgtt
     2941 ctctccatca tattttgttt ttatttgatt aaaagccaca atacctaaat ttcgcaacca
     3001 tgccttaatc agttcaaata ttggcttatc aaaaaaagac tcgcaatttg ccatattaaa
     3061 gatgctgata tcattagcta tattattatt tatcaaaaca caatcaccaa taccatcaat
     3121 atacacagat ctaagtattt tagctgaaag cagatttttt agaacagatt cataacttaa
     3181 atgcttggcc tgctttatcg gagagccaga cacaatcccg aaatccttta ccttcaagta
     3241 tccgccatta ttaattacgg aaagtatagc gtgagaatac gccgaatttg cctcacgcat
     3301 acattttttg agcgcccgcc aaaatttgaa gctaccaaag tcatctttta gataaatgaa
     3361 tctcctgtta tgactaaatc ttaaaaatgg caacttacca atatcagtgc ttcgagagat
     3421 cattttacgt attgcatcag atgacataga aggattctga cgcttaatcc tctcagcaag
     3481 ttcagaagat atgcacggac cataactctc aagcacttgt ctaacaatgt tttttttcat
     3541 aaattcatct tatttttgtc acactacgct atttataaaa ttacactacg tgtaatatct
     3601 atagatccc
//
LOCUS       DAJOWB010000247         3558 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJOWB010000247 DAJOWB010000000
VERSION     DAJOWB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.248"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..719)
                     /locus_tag="OHO85_004801"
     CDS             complement(<1..719)
                     /locus_tag="OHO85_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCQ2674028.1"
                     /translation="MARSCVTDPRWRELVALYRYDWIAAADVLFGKTPTWQQDEIIES
                     TQQDGSWTSVTSGHGTGKSDMTSIIAILFIMFFPGARVILVANKRQQVLDGIFKYIKS
                     NWATAVSRFPWLSKYFILTETSFFEVTGKGVWTILIKSCRPGNEEALAGEHADHLLYI
                     IDEASGVSDKAFSVITGALTGKDNRILLLSQPTRPSGYFYDSHHRLAIRPGNPDGLFT
                     AIILNSEESPLVDAKFIRAKLA"
     gene            complement(719..1912)
                     /locus_tag="OHO85_004802"
     CDS             complement(719..1912)
                     /locus_tag="OHO85_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCQ2674029.1"
                     /translation="MTWDDHKKNFARLARDGGYTIAQYAAEFNLNPNTARRYLRAFKE
                     DTGTADSRKPNKPVRKPLKSMIIDHANDQRAGDHIVAEMAEKQRVNAVVSAAVENAKR
                     QNKRINDRSDDHDVITRAHRTLRDRLERDTLDDDGERFEFEAGDYLIDNVEARKAARA
                     MLRRSGADVLETTLLEKSLSHLLMLENARDTCIRLVQEMRDQQKDDDEGTPPEYRIAS
                     MLNSCSAQISSLINTIYSIRNNYRKESREAEKHALSMGQAGIVKLAYERKRENNWSVL
                     EAAEFIEAHGGKVPPLMLEQIKADLRAPKTNTDDEERQTAVGGPSLEDLDKVARERAA
                     NRRADAALWIEQRREEIADIVDTGGYGDVDTEGVSNDPWLEQDLDEDEEEDEEVTRKL
                     YGDDD"
     gene            complement(1999..2451)
                     /locus_tag="OHO85_004803"
     CDS             complement(1999..2451)
                     /locus_tag="OHO85_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Late promoter-activating protein"
                     /protein_id="HCQ2674030.1"
                     /translation="MLLNWQGRHFMEINHSRITSYEIADYMIRTKSLLSAKELAAILE
                     KEYPHLDVDKRDVYLRLKAIAVSKYSSVLIDDSTRPRRFQIHSLNPEFFRRSRAPRRF
                     DEKLQNELYMTQDEKERREHQPWVMARQLFNKVVRQHRHYGNATSARI"
     gene            complement(2540..3478)
                     /locus_tag="OHO85_004804"
     CDS             complement(2540..3478)
                     /locus_tag="OHO85_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCQ2674031.1"
                     /translation="MEPVPRELIEHMEDGLVVTEQTMATNEALQPFFNSDELFRRIGG
                     IDALVAWLRRKEGQCQAADRSWCDNHIVHAERDNSAVLLCWHHDNHYRMRGFNELKET
                     LHNNRVNWILDVARQEMGLSNSHDLSIQELCWWAFMRNMMHLMPEEVCRISINKMKAT
                     PQDSGPLKEADIRPYDDRATAYVQMMEERAAPMRAKVCPVDVDSDPGMAHFKIPKLQS
                     LKLPEYVDFVASRPCCGCGAAGAGAHITPYIVRHSRLCAHDIYAIPLCQSCQRDIERD
                     RDNWEKTHGRLAMHQRLFFDYALGVGAITSHSSSVR"
BASE COUNT          853 a          950 c          813 g          942 t
ORIGIN      
        1 gcaagttttg ctcgtataaa ttttgcatct acaagcggag attcttcact attcagtatt
       61 atcgcagtaa acaatccatc aggatttccc gggcgaatag ctagtctgtg gtgtgaatcg
      121 tagaaatagc ctgaaggtcg cgtaggctgg gaaagaagca gaatacggtt atccttaccg
      181 gtcagcgcac ctgttatcac actgaatgct ttatcactca cacccgacgc ttcgtcgatg
      241 atatacaaga gatgatcggc gtgttcacca gccaacgcct cctcatttcc ggggcgacag
      301 gactttatca atattgtcca aacacccttg ccagtcacct caaaaaaaga cgtttctgta
      361 agaatgaaat acttcgacaa ccacgggaat ctgctaacag cagtagccca attgctcttt
      421 atgtatttga aaataccatc aaggacttgc tgtcttttgt tagcgaccag aatgacgcga
      481 gcgccgggga aaaacatgat gaagagtatt gcaatgatac tcgtcatatc cgatttacca
      541 gtaccatggc cggaggtcac acttgtccaa ctgccgtcct gctgcgtgga ctcaatgatc
      601 tcatcctgct gccaggttgg tgtcttccca aacaacacat cagcggccgc aatccagtca
      661 taacgatata gcgccaccag ctcgcgccaa cgtgggtccg ttacgcaact tctggccatt
      721 aatcatcatc cccgtatagc ttgcgggtaa cttcttcgtc ttcctcctcg tcttcgtcca
      781 ggtcttgttc cagccatggg tcgtttgata caccttcagt atcaacatct ccataaccgc
      841 ctgtatcaac gatatcggcg atttcttccc tacgctgctc aatccacaat gcggcatcgg
      901 cgcggcggtt ggcggcccgt tctcgcgcaa ctttgtccag atcttcaaga gaagggccac
      961 cgacggctgt ttgcctttcc tcatcatcgg tattggtctt aggagcacgc agatcggctt
     1021 tgatttgctc cagcatcagg ggcggtactt tccctccatg cgcctcgatg aattcagctg
     1081 cctccagcac tgaccagttg ttttcacgct ttcgttcgta tgccagctta acaatgccag
     1141 cttgccccat agacaaagcg tgcttttccg cctcccggct ttcttttcga tagttattcc
     1201 ggatgctgta aatggtgttg atcaggctgc ttatctgcgc ggaacagctg tttagcatgc
     1261 tcgcgatacg gtattcaggc ggagttcctt catcatcgtc tttttgctga tcgcgcattt
     1321 cctgaaccaa gcgaatacac gtatccctgg cgttctccag cataaggaga tgagagagag
     1381 acttttccag aagagtggtt tccagaacat cagccccgga ccgacgcaac atagcgcgcg
     1441 cggccttccg cgcttcaacg ttatctatca ggtaatcgcc agcttcgaat tcaaagcgtt
     1501 caccatcatc atccagggtg tcgcgttcca ggcgatcacg taaggtccgg tgggcgcggg
     1561 tgatcacgtc atgatcatca gaacgatcat ttatgcgctt attctggcgc ttcgcgttct
     1621 cgactgcggc actgacaaca gcattaactc tttgtttttc agccatttca gccacaatgt
     1681 gatcacctgc acgttgatca ttagcgtgat caatgatcat gctttttagt ggttttctga
     1741 caggcttatt tggcttacgg ctgtccgctg ttccggtgtc ttctttgaat gcacggagat
     1801 aacgacgtgc ggtgtttggg ttgagattaa actcggcggc atattgtgcg atggtgtaac
     1861 caccatctcg cgccaggcga gcaaaattct tcttgtgatc gtcccaggtc acttatgctt
     1921 cctttcgtat aaaactcttt ttgacgcgag ggtaacgaaa gtcacatgtc aaaaggcccg
     1981 gaacgggcaa gcaatcaatc agatacgtgc ggatgtggca ttaccgtaat gacggtgctg
     2041 acggaccacc ttattgaaaa gttgacgcgc catcacccaa ggctggtgct cccggcgttc
     2101 cttttcgtcc tgcgtcatat agagttcgtt ctggagtttt tcatcaaacc ggcgcggagc
     2161 gcggctgcgg cgaaagaatt caggattcag agagtggatc tgaaatctac gtgggcgtgt
     2221 actgtcatca atcaaaacag acgaatactt agacacagcg atagccttta agcgcagata
     2281 aacatcgcgc ttatcgacat ccagatgcgg gtattccttt tcaagaattg ctgcgagttc
     2341 tttcgctgat agaagagatt tagtgcggat catgtaatcc gcaatctcgt acgatgttat
     2401 tcgtgagtga tttatttcca tgaagtggcg tccctgccag ttaagtaaca tcctgtcacc
     2461 tactgattag cccatgtcaa ctaatcaacg tggaatataa taccctcgat taaataaata
     2521 gcaatacatt agagcaattt tatctaacgc tcgacgagtg acttgtgata gcgccgactc
     2581 caagcgcgta atcaaagaac aatcgttgat gcatcgccag cctaccgtgc gtcttctccc
     2641 aattatcgcg gtcacgctca atatcacgct ggcatgactg gcacagagga atagcataaa
     2701 tgtcatgcgc gcataatcga ctatgacgaa cgatataagg cgtaatgtga gcgccagctc
     2761 ccgcagctcc acagccacag catggacggg aagccacaaa gtccacgtac tcgggcaatt
     2821 ttagcgattg aagttttggt attttgaaat gcgccatacc tgggtcggag tcaacatcca
     2881 cagggcatac ttttgcacgc atcggcgcgg cgcgttcttc catcatctga acatatgctg
     2941 tagcgcgatc gtcatacggg cgaatatccg cctctttcag aggtccgcta tcctgcggag
     3001 tagccttcat cttatttatt gatatgcggc agacttcttc cggcatcagg tgcatcatgt
     3061 tgcgcatgaa agcccaccag cacagctcct gaatacttaa atcatggcta tttgaaaggc
     3121 ccatttcctg acgggcgaca tccagtatcc agttaacgcg attattgtgc agcgtttctt
     3181 tcagctcatt aaaaccacgc atccggtaat ggttatcgtg atgccagcac aacaacaccg
     3241 cgctattgtc tcgttcagcg tggacaatat ggttgtcaca ccaactacga tctgcggcct
     3301 ggcattgacc ctctttccta cgcaaccacg ccaccagcgc gtcaattcca ccaatacggc
     3361 gaaacagttc atcgctgtta aaaaacggct gcaacgcctc atttgttgcc atggtttgct
     3421 cggtaacaac gaggccgtct tccatgtgct caattaactc acgcggcacc ggctccataa
     3481 taaatttacg gccagcctcc accagctttc tgacctcctg atccactttg aacgtggcga
     3541 ggccaagctc tttctgta
//
LOCUS       DAJOWB010000248         3500 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJOWB010000248 DAJOWB010000000
VERSION     DAJOWB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.249"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..240
                     /locus_tag="OHO85_004805"
     CDS             <1..240
                     /locus_tag="OHO85_004805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674032.1"
                     /translation="DNFDDAVECCADLGIPFFLDAGNKKLVFWFVRVDDEGYPEIARC
                     TEREFATILAGISAGGMYCPECGTVHWPDGVTPPV"
     gene            240..502
                     /locus_tag="OHO85_004806"
                     /pseudo
     CDS             240..502
                     /locus_tag="OHO85_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706936.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
     gene            707..1717
                     /locus_tag="OHO85_004807"
     CDS             707..1717
                     /locus_tag="OHO85_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000487120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCQ2674033.1"
                     /translation="MFQLGIDVSKKTLDLCLLREGVKGSVKTRKLQNDINAVNAVIAW
                     LSKQHCKPEDVHIIMEATGVYHESLAYGLHKAGGRISLANPHRAREFARGMGMLTKND
                     RVDAYMLACYGALKAPEPWLPPPEEVRHLSALLRRRDALVADSTREKNRLEKYLATDT
                     PSVIAESVEAMIGQLNAEIKRLDRLIRSHLNQHPELKKDFDLLTSIKSVGYQLGLNML
                     VILRSHDFATAEQAAAFLGVVPVEKYSGTSVRGCPKLSKIGPPEIRAKLYLASLCGLR
                     FNPLMKAMYERLCLRGKGKMCAIGALMRKLVHWCYGVLKTQKPFDVGYLSSYARSACK
                     AA"
     gene            2295..2885
                     /locus_tag="OHO85_004808"
     CDS             2295..2885
                     /locus_tag="OHO85_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCQ2674034.1"
                     /translation="MSRIFAYCRVSTLEQTTENQRREIESAGFNVRPQRIIEEQISGS
                     VAASERPGFNRLLDRLESGDVLIVTKLDRLGRNAMDIRKTVEQLTESGIRVHCLALGG
                     VDLTSSAGKMTMQVISAVAEFERDLLLERTYSGIARARDAGKRFGRPPLLNEEQKQKV
                     LERIKSGASISAIAREFRTTRQTILRVRAKQQTVTV"
     gene            2932..3090
                     /locus_tag="OHO85_004809"
                     /pseudo
     CDS             2932..3090
                     /locus_tag="OHO85_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3240..>3500
                     /locus_tag="OHO85_004810"
     CDS             3240..>3500
                     /locus_tag="OHO85_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCQ2674035.1"
                     /translation="MKKVLCALGLMFTAVSSALATTYPLTIENCGYQETFTRPPERVV
                     ALGQNTVEILLLLGLQKQVVASAFWPTSVLPQLAEQNAKIKTL"
BASE COUNT          919 a          801 c          929 g          851 t
ORIGIN      
        1 gataattttg acgatgccgt tgagtgctgc gctgacctgg gtattccgtt ctttctggat
       61 gcaggaaaca aaaagctggt cttctggttt gttcgtgtcg atgacgaagg gtatccggaa
      121 atagcccgct gtacggagcg ggagtttgca accattcttg ccggtatcag tgccggtggt
      181 atgtactgcc cggaatgcgg cacagttcac tggccggatg gcgttacccc acccgtctga
      241 tgcttccccg ttttgccgat atttttcagc agggtaaccg ctggcttaac tggctggaga
      301 aacagccgga aggttcagtg cgtccggtgg tgactgagtc agtgacaaaa atcatggcat
      361 gcgggaccac gctgatgggc tacacgcaat ggtgctgttc gtcaccggac tgttgccaca
      421 ccaaaaagat ctgcttccgg tgtaaagccg ctcctgtccg aactgcgggg tgaaggctgg
      481 cgcacagtgg atacagtatc ttgtatgtaa taggacttga tggctagcta ccatcatatg
      541 aagcagagtg agcttgtccc agccctgaac agtcagactt tgattagcag caaaagccct
      601 ctgcttcacc tttcacgcca gcattaacat tgaacggttg ccaagagcga tgactctgca
      661 tgtacaaggg gaataggcgt gatggtttgg tgaaaggaga atattcatgt ttcagttagg
      721 tattgatgtc agtaagaaaa cgctggattt gtgcctgctg cgcgaaggcg taaaaggcag
      781 cgttaaaacc cgaaagcttc agaatgatat taatgctgtc aacgcggtaa tagcctggct
      841 cagtaagcaa cattgtaaac ctgaagatgt ccatatcatc atggaagcca ctggtgttta
      901 tcatgaatca ctggcttacg ggcttcacaa agccgggggc cggatatcac ttgccaaccc
      961 acacagggca agagagtttg cccggggtat gggaatgctg accaaaaatg accgggttga
     1021 tgcttatatg ctggcttgct atggcgcgct taaagcaccg gagccatggc ttcctccacc
     1081 ggaagaagtc cgccatctca gtgcactgct ccgcagacgt gatgctcttg tggctgacag
     1141 cactcgcgaa aaaaacaggc tggaaaaata cctggcaaca gacacacctt ctgttatagc
     1201 tgaatccgta gaggcaatga ttggtcagtt gaacgcagaa attaaacgtc tggacagact
     1261 gatccgttca caccttaacc agcatcctga attaaaaaag gattttgacc tgctgacgtc
     1321 aataaaatcc gtaggttatc agttaggtct gaatatgctt gtcatcctgc ggagccatga
     1381 tttcgccaca gcagaacagg cggctgcatt tttaggtgtc gttcccgttg aaaaatattc
     1441 cggcacctcg gtcagagggt gtccgaaact gtcaaaaatt ggtccgccgg aaatccgggc
     1501 gaaactgtat ctggcttcgt tgtgtggtct gagatttaac ccattgatga aagcgatgta
     1561 tgagcgatta tgcctgagag gtaagggcaa aatgtgtgcc attggtgcgc tgatgagaaa
     1621 actcgtgcac tggtgctatg gcgtactgaa gacccaaaaa ccgtttgatg tgggctattt
     1681 gtcttcatat gcgcgtagcg catgtaaggc ggcatgatgt agttacatat ggtacaggag
     1741 taacaccgcc gaagcccggc gtaagccggt acgtattgat aggtttcacc tatcccatcc
     1801 ctgccctgcg aggccatgcg ggctttcagc gatgatgaag tacaggtaag cccttttaac
     1861 cccgttagcg cggctacgcc gcgctgtatc ggggacttag tgctgacagc ttgctggcag
     1921 tacttagggg gctgtacgtc ggagttcaag cgatttttct tgacagaaca cggtagctgc
     1981 taagccatac ccgctttcag ccatgagaga gcttctgtgc gcggtcggag tggtcccgac
     2041 gagggtttac ccgaagtcgg gacgtatctc cgcgttagcg gggtcagttt ggaattcgga
     2101 aaataaacca tgttaagact attttttagc caaagggctt tcaggaatcg ccagaatggc
     2161 gggacaacag tggttttggt gtccgaaatc gaccggtttc ggacattgtt aaacagatat
     2221 atccgaaagt cagcgaaatt actttcggac atgcattata atatgtacac ccatttcgta
     2281 cagaagatct cactatgtct cgaatttttg cctattgtcg ggtttccact ctggaacaga
     2341 cgaccgaaaa tcaacgtcgg gaaatcgaaa gcgcgggttt taacgtcaga ccccaacgaa
     2401 taatcgaaga acaaattagt ggatcagtag cagccagtga acgtccaggt tttaaccggt
     2461 tgcttgatcg tctggaaagt ggcgatgtat tgatagtcac aaaactggat cgcctcggtc
     2521 gtaatgcaat ggatatcagg aaaacagtgg agcaactgac cgaatctggc atcagggtgc
     2581 attgcctggc attaggtggt gttgatctga ccagttcggc aggaaaaatg acaatgcaag
     2641 tgatttcagc ggtcgctgaa tttgaacgag atcttttact tgaacgaact tattcaggga
     2701 tagcaagagc acgggatgca ggaaaacgtt ttggtcgccc accgctatta aatgaagaac
     2761 aaaaacagaa ggtactcgaa cgcattaagt ctggggcaag tataagtgct attgccaggg
     2821 aattccgaac cacacggcaa acaattctga gagttagagc gaaacagcaa actgttactg
     2881 tatagaaaat agtcttagcg tgggatattt tccgttttcc aagcggaccc caaggaagac
     2941 tacatcgcgt tcatgccgaa accgaatgta agaacggcat tgcataatct tgcagtggcg
     3001 atcgaacatt acaatgaaaa ccatccgcac agtgcgttgg gttatcgctc tccgcgagaa
     3061 tatcgacgtc agcgggtaac attaacttaa gatacaaccc ctgtctggaa ataagggggc
     3121 aagaacacat tgacactata aatatggcat tgtataaaac cccgcgataa taatgagttt
     3181 catttgcatt actcccattt gctggtcatc cactcgcctg tttacaagga tagcgtttaa
     3241 tgaagaaggt cctttgcgcg ttaggcttga tgttcaccgc cgtcagttcc gcgctggcaa
     3301 caacctaccc cctgacgatt gaaaactgcg gctatcagga gaccttcacc cggccgccgg
     3361 agcgcgtggt cgcgctgggg caaaatacgg tcgaaattct gctgctgctc gggctgcaaa
     3421 agcaggtggt cgccagcgcc ttctggccga ccagcgtttt accgcagctg gctgagcaaa
     3481 acgcgaagat taaaaccttg
//
LOCUS       DAJOWB010000249         3497 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJOWB010000249 DAJOWB010000000
VERSION     DAJOWB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.250"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            52..729
                     /locus_tag="OHO85_004811"
     CDS             52..729
                     /locus_tag="OHO85_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCQ2674036.1"
                     /translation="MMAPTIYHRIDGTKYRNVWVVGDLHGCYTRLMSELHRVDFDPAQ
                     DLLISVGDLIDRGTENVESLELLQMPWFRAVVGNHELLMLDALSPDGNVNNWLMNGGQ
                     WFFMLDTDQEILAWALVELVKRLPYIIELNTGQETIVIAHADYPDNEYQFGKEVPLFN
                     VVWARERISDSMDDIGGEISGADRFIFGHTPVKSPKTFWNQQYIDTGAVFCGNLTLMK
                     VKGDGAA"
     gene            726..1427
                     /locus_tag="OHO85_004812"
     CDS             726..1427
                     /locus_tag="OHO85_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674037.1"
                     /translation="MKIALVFRSGGDYNASDVQWLVNQLPKDYEIICLTDLKCLHVPG
                     VKVIPLINQWQKCRGWWAKIELFRPDITDDLFYLDLDTVIAGDIRPILEHPPTSFTML
                     RDFYHPQYRGSGALWIPNSVKAHIWSSFWQDPEGWISRCVTTECWGDQGFLRKVMGDD
                     TPAFQDLYPGWFVSYKADVVEPGSKYASARYSRGNGALPKDCRIIFFHGKPRPREVSE
                     DWLPLISSFFERESE"
     gene            1729..2991
                     /locus_tag="OHO85_004813"
     CDS             1729..2991
                     /locus_tag="OHO85_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674038.1"
                     /translation="MSTSAQNQSIENVCIPDVLNAGIPAIIQNIRAAQRRVSCDDLTA
                     RFFDNAVQSAEMLHAQLIDVYNAEADSHNSLVDAAENMQLDLGLKGKEIEELQLEIEH
                     LKRQQQDAIDDATHDANQRADNAERISIELETKLNEMTAMVELRNSQISTLKSQYKEI
                     MKLDPFNLEKRYNKAKSERQELRKQVADLNQQLKKTIKDASEARVAFANKKAEVTALV
                     NENAKFATLKKEMYGITERRFPASKLHPTLGQISFFPRLLAYGISSPKEFNNERPYIV
                     SKLDFAYQFCCDMGYAIDIRINEWLMPNFQPLAIFREFQPEGWVEFFHELICKEMESR
                     RPELVRRVEWAQEVMLADAELPFEPEFIDDLATKGLHTLFDVVTRRHEQLVVELGLEE
                     TAARRLLDVCYARSDAWEKENGGTIYVR"
     gene            3064..>3497
                     /locus_tag="OHO85_004814"
     CDS             3064..>3497
                     /locus_tag="OHO85_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-phosphatase"
                     /protein_id="HCQ2674039.1"
                     /translation="MSIFNKHAHQERPYIVIVDIDGTISEATEDRLHLLPPPGKGALT
                     EHWNEFNLACDTDAPITPVIDMVRQLSGIYTLWFVTGRCEIARDKTRAWLRKHVTNGA
                     EPLLSMRPATDDRNDGPAKIDLLKKIGLSKIAFALEDKIEVAR"
BASE COUNT          947 a          765 c          889 g          896 t
ORIGIN      
        1 gaaaatggga ggtattccgc agatgactct taaagaatgg ctgaagaata catgatggca
       61 cccacaattt atcaccgtat cgacggtacc aaatacagga atgtctgggt tgttggtgat
      121 ctgcatggtt gctacaccag actgatgtcc gaactccatc gtgtggattt tgacccggcg
      181 caggatttac tgatatcggt cggcgacctt atcgatcgcg gtactgaaaa tgtcgaatct
      241 ctggaactat tgcagatgcc ctggttcagg gcagtagtgg ggaaccatga gctgttgatg
      301 ctcgatgcgt taagtcctga tggcaacgtg aataactggc taatgaatgg cggacaatgg
      361 ttcttcatgc tggacactga tcaggaaata ttagcctggg cgctggtgga gctggtaaag
      421 cgtctgccct atatcattga gttgaacacg gggcaagaaa ctatcgttat agcccatgcc
      481 gactatccgg ataatgaata ccaattcggt aaggaggtgc cgcttttcaa cgttgtctgg
      541 gcgcgcgagc gtatcagtga ttcgatggat gatattggtg gcgaaatttc gggcgcagat
      601 cgttttatct ttggtcacac tccggtgaaa agcccgaaga cattctggaa tcagcagtat
      661 atcgacactg gtgccgtatt ttgcggaaac ctgacattga tgaaagtgaa aggtgatggt
      721 gcagcatgaa gattgcttta gtttttcgct ctggtggtga ctataacgct tccgatgtgc
      781 agtggctggt taatcaactg ccaaaagact atgaaattat ttgcctgaca gacctgaagt
      841 gtttacatgt acctggcgtc aaagttatcc cattgatcaa ccagtggcaa aagtgccgtg
      901 gctggtgggc gaaaatcgag ttgttccgac cggatataac cgatgatctg ttctatctgg
      961 atttggacac ggttattgcc ggtgatatac gcccaatcct agagcatcca ccaaccagct
     1021 tcaccatgct tagggatttt taccatccac aatatcgtgg cagcggtgcc ctgtggatac
     1081 caaatagtgt taaagcgcat atctggagtt cattctggca agatccggaa ggttggattt
     1141 ctcgttgtgt cactactgag tgctggggtg accaggggtt cttacgaaag gttatgggcg
     1201 atgatacacc agcatttcag gatctgtatc caggatggtt tgtaagttac aaggccgatg
     1261 ttgtggaacc tggttcaaaa tatgcgagcg cgcgttactc cagggggaat ggggcattac
     1321 caaaagactg ccgaataatc tttttccacg gcaaaccgcg acctcgcgaa gtgtcagagg
     1381 attggcttcc ccttatcagc tcattttttg agcgagaatc agaataatat tgctctaata
     1441 attccatatt tttaaaacgt gatgtacact catcacgttt tttattagag caatctacaa
     1501 ggtgcactat gtggccattc cgacggaaat atcactactg gctgatcgcc tttgttacgc
     1561 cgaccggcgg tatcaggcat gtcatcacca ggtatcgcaa caagagactc accttagcca
     1621 gaattttaca ggctgccata ggtgagggac tggatacaaa ttgcgtagtc cttcctcctt
     1681 catacttagg aaaaatgacc gaagcacaag ctaatacgga actttgaaat gagcacttca
     1741 gcacaaaacc aatcaatcga aaatgtatgt atccctgatg tcctgaatgc cggtatcccg
     1801 gccattatcc agaacatccg ggccgcgcaa cgccgcgtta gttgtgatga cctcacagca
     1861 cgtttttttg ataatgcggt tcagtcagcg gagatgcttc acgcacagct tattgatgtt
     1921 tataacgcag aagctgatag ccataactcc ctggtagatg cagctgaaaa tatgcagttg
     1981 gatctcggtc tgaagggtaa agaaattgaa gagcttcagc tggaaattga acatttgaaa
     2041 cgccagcaac aggacgcgat cgacgatgcg acgcatgacg ccaaccagcg tgctgataat
     2101 gccgaacgta taagcattga gctggaaaca aaactcaatg aaatgaccgc gatggttgaa
     2161 ctgcggaact cacagatttc aacgctaaaa tctcaatata aagagatcat gaaacttgat
     2221 ccttttaacc ttgagaaacg ctataacaaa gctaaaagcg agcgacagga actgcgtaag
     2281 caggtcgccg accttaacca acagctcaaa aaaactatta aagatgcaag cgaggcgcgc
     2341 gtggcatttg ctaataaaaa agcagaggtt accgcgctgg ttaatgagaa tgccaaattt
     2401 gcgacgctca agaaggaaat gtatggcatt actgagcgcc gtttccctgc aagcaaactt
     2461 catccgacgt tagggcaaat ctccttcttc ccgcgcctcc tggcttatgg gatctcatcg
     2521 cctaaagagt tcaataacga gcgtccttat atcgtttcta agctggactt tgcttatcag
     2581 ttctgctgcg acatgggcta tgccattgat atccgaatca acgaatggtt gatgccaaac
     2641 ttccagccgt tggcaatttt ccgcgagttc cagccggaag gttgggtaga gttcttccat
     2701 gaattgatct gtaaagagat ggaaagccgt cgcccggaat tggtccgtcg agttgagtgg
     2761 gcgcaagagg ttatgttggc agatgcagag ctgccgttcg aaccggaatt cattgatgat
     2821 ctggcaacta aagggctgca taccctgttt gatgtggtta cccgccgtca tgagcagttg
     2881 gttgtcgaat tgggtttaga ggaaactgcg gcaagaagac ttctcgatgt ttgctatgca
     2941 cgtagcgatg catgggaaaa agagaacggc ggcactattt acgttcgctg atagttacag
     3001 tgtcactttt aatgctggtg gagtgctccc accagcattt ttttcgtcca atgaggaggg
     3061 catttgagta ttttcaataa acacgcacac caggaacgtc cgtacatcgt catagtagat
     3121 attgatggga cgatatcaga ggcaacggaa gacaggctgc atttacttcc accacctggc
     3181 aaaggtgcat taacagagca ctggaacgag tttaaccttg cctgtgacac tgatgctccc
     3241 atcactccag ttattgatat ggtgcgccag ttgtccggca tttacaccct ctggtttgta
     3301 accgggcgct gtgagatagc cagggataaa acacgagcct ggttgcgtaa gcacgtaaca
     3361 aatggggcgg agcctttgct atctatgcgt cctgccaccg atgacagaaa tgacggtcca
     3421 gcaaagattg atctcctgaa gaaaattggt ctaagtaaaa ttgcgttcgc gctggaagat
     3481 aagattgaag tggcgcg
//
LOCUS       DAJOWB010000250         3469 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJOWB010000250 DAJOWB010000000
VERSION     DAJOWB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.251"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            133..1425
                     /gene="sctN"
                     /locus_tag="OHO85_004815"
                     /pseudo
     CDS             133..1425
                     /gene="sctN"
                     /locus_tag="OHO85_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311757.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SctN"
     gene            1415..1831
                     /locus_tag="OHO85_004816"
     CDS             1415..1831
                     /locus_tag="OHO85_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HCQ2674040.1"
                     /translation="MLSKVNRLIRRTAQSLAACEASLQKLNAEKEKLAEKERLYDMQL
                     KNLKSLLDKKELLGEVVFRQDIFYSLRKVAVIQQQIAEINLEKQKIAERRKILNKEIV
                     QQQAQRKHWWLKGEKYVRLKTRIKKTFKSDASSRRA"
     gene            1959..2722
                     /locus_tag="OHO85_004817"
                     /pseudo
     CDS             1959..2722
                     /locus_tag="OHO85_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314987.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            2722..>3469
                     /locus_tag="OHO85_004818"
     CDS             2722..>3469
                     /locus_tag="OHO85_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
                     /protein_id="HCQ2674041.1"
                     /translation="MFGLRKVNRNTHMFETTFQNWKENGEDVALLMPEFSAKWLPIAE
                     ESGSWSGWVLLGEIFPLISSELAGMALMPETERLIGEWLSLSSSPLNLKHPELKYNRL
                     RVGKVFDGVLSPAQPLIRIWTGELNLWLDKVTVCQYGNAPALDKKSLYWSIHFVIGFS
                     KTCYRSLIDIEVGDVLLISNNLAYAVIYNTKICDLIYPEELKMADHFEYEEDFETDDF
                     DIKKNESEIYDENDDQMINSFEDLPVKIEFV"
BASE COUNT         1208 a          589 c          765 g          907 t
ORIGIN      
        1 ttatcaaaaa acttattgaa tctagttatc gagatctcga agtactttcg tacggggaac
       61 taacggaaaa tgttcccgtt aatattctga aaactattta ggataatgtc ttgcgatatg
      121 gagcatgccg gtatgaaaag attaaaatta ttaaataaat acagctactt acatagcata
      181 aacggctctc tgattgaagc agaacttgac gacgtttcag tgggtgaagt atgcgaaatt
      241 tatgcaagct ggcaagcaaa tgaacgtata gccagggctc aggtcgttgg ctttcgcaat
      301 ggaaagacat tattaaacct tataggcagc tcagtggggc ctacacgtac tgcagttttg
      361 aaaccaacgg gagagcagtt aaccatacag atcagtgatg cctttctttg ttcggttttg
      421 aatgcctctg gtcaaattat ggaaaggttt gtcccgaacc ctccaggcga tagaggaaat
      481 ttacgcctga ttgatgaact gccaccgtcg tatcaggaac gtcgagttat taatacacca
      541 ctagagacac gcataagagt cattgatggc gtactgacat gtggcattgg tcaacgtgtt
      601 gggatttttg catcggctgg ctgtggcaaa acggttttaa tgcatatgct cgtaaacaat
      661 actgaagctg atgtgtttgt tattggcttg attggcgaac gtggtaggga agatacggaa
      721 tgcgcggaat cgatgaaaca atcagtaaat gcggcaaaat gcgtattggt ttatgccacc
      781 tctgatttct cgtcagtaga tcggtgtaat gccgccctga tggcgacaac cgtggcagag
      841 tattttagag atcgaggaaa gagagttgta ctttttatcg actcaatgac gcggtatgca
      901 tgagcactac gtgatatgaa actggcagcg ggtgaaccac ctgccagagg tggttatcct
      961 gcatctgttt ttgacagttt gcctcgctta ctggagaggc cgggaccaac actaaaagga
     1021 agtatcaccg cgttttatac cgtgttactg gaaggggaag acgaatcaga ccccctggga
     1081 gatgaaattc gctcaatcct cgatggccat atctatctga gtcgcaaact cgctgggcaa
     1141 ggtcactatc cggcaataaa tgttctaaaa agtgtgagtc gagtatttgg tcaggtcact
     1201 gatgaaaaac acagagataa tgctgcacgg gtaaggaaaa tcctcacaac tttggaagat
     1261 ctacaggttt ttattgactt aggggaatat cgcgctgggc aaaatgctga aaatgatttc
     1321 gctatgaatg cacggcctaa actcacaaat tggttgaaac agtcagttaa tgaaaaaatg
     1381 ccaatgagtg aaactctaaa agagcttgaa cgaattgtta agtaaagtta atcgacttat
     1441 cagaagaaca gcgcagagcc tggctgcatg tgaagcatca ttacaaaagt taaatgcaga
     1501 aaaagaaaag ttagccgaaa aggaacgcct gtatgatatg caacttaaaa acctgaagtc
     1561 actgttagat aagaaggagt tactcggcga agttgtattt cgacaggata tattttactc
     1621 gttacgtaaa gttgcagtca ttcagcagca gatcgctgag ataaatcttg aaaagcaaaa
     1681 aatagcagag cggagaaaaa tacttaacaa ggaaatagtt caacagcaag cacaaagaaa
     1741 acactggtgg ctaaaaggag aaaagtacgt tcgactaaaa acgcgcatca aaaaaacttt
     1801 taaatcagat gcttcatcaa gacgagcttg aacaggagga aaaatataat ggacgaagtc
     1861 aagaaaattg aacacggtaa aattttaaac acagacacac ccgataattc tgcttcgaat
     1921 ttagacagtt tcttaaagaa aaacaaaaaa ataaagaaat gagtgagatt gtcgctggaa
     1981 ccattctcca gttattacgt cagactcagg atagccaatt ttctacattg gggaaaaaga
     2041 atgggcaaat gaagtctatt gacaacaaat catctctcca ggttgacagg aattcctctc
     2101 tttctttgca aacaaatgga acagaacaat tttctacaaa gaaaattctc aatatgactt
     2161 ctcgagatca gggtattagt tctgaactat ctaataaaac gatacaaatt aatgaaaaaa
     2221 tccataatgg tatccataca gaatacatca cagatcaaaa aacagtaata ataaagatcg
     2281 tgaaaaaaaa taccgcgatg gagacaaaat aaatggaccg caggcccatt cattagacag
     2341 tactaacgaa agaaggtttg ctgataacag aaccatgttt actcaacata tcgaaaagca
     2401 aaggaatgtt aatacgctca atcagaatga tataaataat tctgttaata atgccaatgt
     2461 gcacgaaaat gaattgacat atcagtttca gcgttgggga caaaatcata cagtccggat
     2521 tctggaaagt agtgaaggga tacgactaaa accatcagac acacttgtct ccaacagact
     2581 ccgtgaagcc caacacaatg atgttacagc acaacgttgg gtattgactg agcaagatga
     2641 acgacaagga caacgacatc agccgtatga ggagcaagaa aatgaaagca aattcgaaaa
     2701 cgatcagaag gatgaaagtt aatgttcggt ctgaggaaag taaatcgaaa cactcatatg
     2761 tttgaaacca cttttcaaaa ttggaaagaa aatggcgaag atgttgcatt attaatgccc
     2821 gaatttagtg ctaaatggct accgatagca gaagagagtg gcagctggtc tggatgggta
     2881 ttgcttggag aaatatttcc cttaatttca tctgagctcg ctgggatggc tttaatgcca
     2941 gaaacggaaa ggttaatagg ggaatggctc agtttatcaa gttccccgtt aaatctaaag
     3001 catcctgaac taaaatataa tcgtttgcgc gtaggtaaag tatttgatgg agtattgagc
     3061 cctgctcaac cactaataag aatatggaca ggggaattga atctttggtt agataaagtc
     3121 acagtctgtc aatacggaaa cgctccagcg ttagacaaaa aatcattata ttggtccatt
     3181 cattttgtaa ttggatttag taaaacatgt tacagaagtc ttattgatat tgaagttggt
     3241 gatgttttat taatctcaaa taatttagct tatgcggtta tttataatac aaaaatttgt
     3301 gatttaattt atccagagga gttaaaaatg gctgatcatt ttgagtacga ggaagatttt
     3361 gaaacagatg attttgatat caaaaaaaac gagagtgaga tttatgatga aaatgacgat
     3421 cagatgatta atagttttga agacttgcct gtaaaaattg agtttgttc
//
LOCUS       DAJOWB010000251         3457 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJOWB010000251 DAJOWB010000000
VERSION     DAJOWB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.252"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(183..2213)
                     /locus_tag="OHO85_004819"
     CDS             complement(183..2213)
                     /locus_tag="OHO85_004819"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674042.1"
                     /translation="MNKFKLKTVDVWDTILRRDCHPDFIKRSTAYYFFLKFHSLHNVD
                     SVDQLFTKRVETERLIGKKNKDLGYDDEYLIQDVMLQWVENYISAEQYDTPAIAHELY
                     SWEIEQEKKHIYLDPGIEDFLAQYPADKTIFLSDFYTSSTDLTELLVSAGLDQSVISD
                     GVSSIDERLNKRSGRLFDFIQQKYQLAGVDWIHIGDNEWSDVQMPTSKGIKSIRYLPA
                     QQHQLREQKEFLWNKNEDLTETITNNILNKYAASKDLSVDFQLGLKTTPLIAGFCLKI
                     LEQAVISKSEKILFFTREGEFFIKAMNILISHLKTNIKEIKLPEIDIIEVSRLATFAP
                     SLQEISIKEMMRVWNLYSTQSISSLFKTLNVAPETFQSFIDKYGIPADEQIQYPWQDS
                     RIQQLFDDSGFKETLWQHVMQQRALLKNYFATKGLTDDINARICVVDVGWRGTIHDNI
                     ALLYPDIHFTGIYLGLQKFLNEQPSNTSKVAFGPDLNHQLEYPHFLDSVAPIEMITNS
                     PSGSVTGYGLENGKIVAIRSVNDDENSAWHNFTKTFQEGILAGMESFSAAVLSYGITH
                     DVVRGYALNIWDVLISGSNKSLTDAFNNLNHNETFGLGGYVKKNHVPTTFEILSSLWN
                     KNNRAALIEFIKANQWSDGIRKRDNLPSLNKYILALTIDLAVFYKRKFYRKY"
     gene            complement(2219..3409)
                     /locus_tag="OHO85_004820"
     CDS             complement(2219..3409)
                     /locus_tag="OHO85_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008926069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HCQ2674043.1"
                     /translation="MANIAWFIPQLIEGSGGHRTMLQHAAYLEKMGHTCTIFLEDKSG
                     KTSGAEVIHKLFGLKFKDVIYDWNNAQPCDAAVATIWYSAPFVAALPFDCKRLYFVQD
                     FEAYFNPVGDAYLMAENSYCYGLKPITIGRWLRYKLQNDFAVESYHFEFGANHNIYKR
                     VPTIQKEKAVCFIYQPDKPRRCSRIGIEALGIVKHCMPDVKIYLYGSSINDNLWFEHE
                     NLGLLDLPKCNELYNKCSVGLCISSSNPSRIPFEMMNAGLPVVEVWRENNLYDFSEDA
                     MLLCKQTPESIAAGIMKILNDADLAASMSSAGQKFMNGRTLETETEGFYKAFNCVMNE
                     QAYTAEVISRSYNKPPFEAPLARPDIANTLTFAHQSLLKRIALRLPLFVRTPLSALYF
                     KLKN"
BASE COUNT         1034 a          699 c          676 g         1048 t
ORIGIN      
        1 tcatacacta aatcagtaag ttggcagcat caccggctga ttcgatgtat ttgcgggaaa
       61 aaaatcggcc cagatccgcg aaattttaat cagcgagtca gctggggaag aaatgacctg
      121 cttattcgca ccttccctaa ctttaaaaaa aagcatctgt cgatgccctg aataaaaagt
      181 aattaatact tgcgatagaa ttttctttta taaaaaaccg caaggtcaat cgtcagtgct
      241 aaaatgtatt tatttagcga gggcagattg tctcttttac gtatgccatc gctccattga
      301 tttgctttaa taaattcaat caatgctgca cggttgtttt tattccataa agagcttaat
      361 atctcgaagg tcgtgggtac atggtttttc ttaacgtatc cacctaagcc gaatgtttcg
      421 ttatgattta aattattaaa agcgtcagtt agagatttat tactaccact aattagcaca
      481 tcccaaatat ttagggcgta gccacgtaca acgtcgtgag taatgccata actcaacact
      541 gcagcggaga aggattccat accggccagg atcccttctt gaaatgtttt tgtgaaatta
      601 tgccacgcgc tgttttcgtc gtcgttaaca ctgcgaatag cgactatctt accattctcc
      661 agcccatagc ctgttacgct acctgaaggg gagttggtaa tcatttcaat aggagcaacg
      721 gaatcaagaa aatgaggata ctccagttgg tgattcaaat ctgggccaaa agcaactttt
      781 gatgtgtttg atggttgctc gttgagaaat ttctgcaggc ccagatatat gccggtaaaa
      841 tgaatatcag gataaagaag agcaatatta tcgtgaatgg tgccacgcca gccgacatcg
      901 actacgcaaa tacgcgcatt gatatcatca gttagacctt ttgtcgcaaa atagttcttc
      961 aaaagagctc tttgctgcat cacatgctgc cagagcgtct ctttgaaacc agagtcgtca
     1021 aaaagttgct gaatacggct gtcctgccat ggatattgga tctgttcatc tgcaggaatc
     1081 ccatatttgt cgatgaaact ctggaatgtt tcaggggcaa cgttaagggt cttaaataaa
     1141 gagctaatgg actgtgtgct atataaattc caaacacgca tcatctcttt tatgctaatt
     1201 tcttgtagag acggcgcgaa ggtagccaga cggctgactt ctataatatc gatttctggt
     1261 aactttattt ctttaatatt agtcttgaga tgactaatca aaatattcat tgctttaatg
     1321 aaaaactcgc cttcacgagt gaaaaagagg attttttcac tttttgatat gacggcttgt
     1381 tccaaaattt taaggcaaaa accagcgatt aacggagttg ttttcaagcc aagctgaaaa
     1441 tcaactgata aatctttaga tgctgcatat ttattaagaa tattattggt gatcgtttcc
     1501 gtgagatctt catttttatt ccagagaaac tctttttgct cacgcagctg atgctgttgg
     1561 gctgggagat accgaatact ttttatacct tttgatgtcg gcatttgcac atcggaccat
     1621 tcattatcgc caatatgtat ccagtcaacg ccggctaact gatatttttg ttgaataaaa
     1681 tcgaagagac gtccacttct cttgttcagt cgctcgtcaa ttgaagaaac gccatctgat
     1741 atgacagact gatccaatcc agcgctaacg agtaactctg ttaaatcagt ggatgaagta
     1801 taaaaatcgg acagaaagat tgtcttatca gcgggatatt gcgcaagaaa gtcctcaata
     1861 cctggatcca gatagatgtg cttcttctct tgctcaattt cccaggaata tagctcatgt
     1921 gctatcgctg gcgtatcata ttgctctgca gagatatagt tttctaccca ttgaagcatt
     1981 acatcctgaa tgagatactc atcatcataa ccaagatctt tgtttttctt accaattaag
     2041 cgctctgttt caactcgctt agtaaatagc tggtcgacgg aatcaacatt gtgtagcgaa
     2101 tgaaatttaa ggaaaaaata atatgcagtt gaccgcttta tgaagtctgg atggcaatca
     2161 cgacgcagaa ttgtatccca tacatcaaca gttttgagtt taaatttatt catctggatt
     2221 aattcttcaa cttaaaataa agcgcgctta aaggtgtgcg cacaaataac ggtaaacgta
     2281 gagcaattct ctttaacaga ctctgatgcg cgaatgttaa cgtgttggca atatcaggtc
     2341 tggcaagagg ggcttcaaac gggggcttat tatacgatcg tgaaatcact tctgctgtgt
     2401 atgcctgctc gttcatcacg cagttgaacg ctttgtaaaa gccttctgtt tccgtttcca
     2461 gggtacgccc attcataaac ttttgaccag ccgagctcat actcgcggct aaatcagcat
     2521 cgtttagaat tttcataata ccggcggcaa tgctttcagg tgtctgttta cacaacagca
     2581 tagcatcttc gctaaaatca taaagattgt tttctcgcca gacttcaaca acgggaaggc
     2641 ctgcattcat catttcaaag gggatgcgag agggatttga agaactaata cagagtccta
     2701 cggagcattt attatatagc tcattacatt tcggcaaatc taataaacct agattttcat
     2761 gctcgaacca gagattatca ttaattgatg aaccataaag ataaatttta acatctggca
     2821 tgcaatgttt aactatacct aaagcctcaa taccaatacg cgaacaacgt cgcggtttat
     2881 ctggctgata gataaagcaa accgcttttt ctttttgaat ggtgggaacg cgcttataga
     2941 tattatgatt cgccccaaat tcgaagtgat agctttcaac agcaaaatca ttttgcagtt
     3001 tatatcgtaa ccagcgccca atcgtaattg gctttaagcc gtaacagtat gaattttcag
     3061 ccatcaggta agcatcgccc accgggttga agtaagcttc aaaatcctga acgaaatata
     3121 aacgtttaca atcaaatggt agagcggcca cgaatggtgc agaataccat attgttgcaa
     3181 ctgcagcatc acatggttgc gcattattcc agtcataaat gacgtcttta aattttagac
     3241 caaataattt atgaattacc tctgcaccgc ttgttttgcc gcttttatct tcaagaaaaa
     3301 ttgtacatgt atgacccatt ttttccagat aagcggcatg ttgcagcatt gtgcggtggc
     3361 cgccagaccc ttcaatgagt tgaggtataa accaagcaat attagccatt ataacctttc
     3421 acaatagaat tattttatat ttagcatgcg tagtaga
//
LOCUS       DAJOWB010000252         3445 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJOWB010000252 DAJOWB010000000
VERSION     DAJOWB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.253"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(1..388)
                     /locus_tag="OHO85_004821"
                     /pseudo
     CDS             complement(1..388)
                     /locus_tag="OHO85_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            456..1393
                     /locus_tag="OHO85_004822"
                     /pseudo
     CDS             456..1393
                     /locus_tag="OHO85_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491235.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1553..2357
                     /locus_tag="OHO85_004823"
     CDS             join(1553..1903,1905..2357)
                     /locus_tag="OHO85_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611700.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HCQ2674044.1"
                     /translation="MAGNKWQISDELWEKMAPLLPEHKTHHPLGTHRRRVNNRAAMNA
                     ILFVLRTGCQWNALNATGICSSSSAHRRFQEWRDAGVFERFWQNGLLACEQLDAIDWS
                     WLSMDGCMTKSPLAGQKTGRNPTERGKQGVKRSLMTDANGLPLSLVVAGANTHDIKLV
                     ADTLDALQTGRPGRRLHLCMDKGYEAEWLEACLKNRRYEPHIQSRKEESEAIKSTDFK
                     AHRWVVERTHSWMNRFRRVLTLWEKKVENYVAMLYLTCGVIVWNKILLG"
     gene            2586..>3445
                     /locus_tag="OHO85_004824"
     CDS             2586..>3445
                     /locus_tag="OHO85_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCQ2674045.1"
                     /translation="MRIVYFVNAAWYFELHWLDRSEAAISKGYEVHLVSNFADDAIKN
                     NLEKKGIKCWDIELNRFSKNVFKNISIFTAFRKICKQIKPDLIHLITIKPILFGGLYA
                     RVAGIPFVVSFVGLGRLFGNKRGWLNKFIFNSVLSIYSLLLSAKSPAQVIFEHNADFA
                     ELSKYIKFDENNIHIIEGAGVDRFRFSYQPEPTQNNFSVLFASRLLWSKGLGEVVEAI
                     GKVKQNENNTTLYVAGILDDKDPDRIELDQILQWEKEGKIIWLGRRDDIQNLIRNSHV
                     VILPTKYSEGV"
BASE COUNT          940 a          763 c          907 g          835 t
ORIGIN      
        1 ctgtcatacg cgtaaaacag ccagcgctgg cgcgatttag ccccgacgta tccccactgt
       61 tcgtccattt ccgcgcagac gatgacgtca ctgcccggct gtatgcgcga ggttaccgac
      121 tgcggcctga gttttttaaa tggcggaaaa tcgtgttgag gccaacgccc ataatgcggg
      181 cggttgcccg gcatccaacg ccattcatgg ccatatcaat gattttctgg tgcgtaccgg
      241 gctgagaggc ggtgtaagtg aactgtagtt gccatgtttt acggcagtga gagcagagat
      301 agcgctgatg tccggtggtg cttttgccgt tacgcaccac gccttcagta gcggagcagg
      361 aaggacatct gatggaaatg gaagccacgc aagcacctta aaatcaccat catacactaa
      421 atcagtaagt tggcagcatt acccatcgtg tcaccatggc aaagcaaaag tttaaaatca
      481 ccaactggtc agcttacaac aaggcactca gacagcgtgg ttcgctgacg atctggcttg
      541 atgaatctgc tattgctgca tggactgact gcgcaaagcc tgaaggtcgt ggtcggcctc
      601 tccactacac cgatatggcg atcaccacgg ttctgatgat gaagcgcgtg tttaatctgc
      661 cactccgggc attacagggc ttcgttgact cgatttttac gctgatggct ctgccgctgc
      721 gttgcccgga ctactctctg gtcagcaaac atgcaaagag cgttaacatc agcataaaaa
      781 cgccaacccg gggtgaaatc tcgcatctgg ttatcgatgc tactggcctg aaggtcttcg
      841 gggaaggcga atggaaagtc aggcaacatg gggctgacag gcgcagagta tggcgcaaac
      901 ttcatctggc tgcagacggt gtgacgcatg agataatctg tgccgattta tcgctcagcg
      961 gtacgacaga tgcgcaggca ctgccggggc tgattaacca gacccaccga aaaataagag
     1021 aagcttcagc agacggcgct tatgacacgc gcaactgtca tgatgcgttg ctgaggaaaa
     1081 aaatcaggcc acttatccct ccacgaaacg gggcgcaata ctggccgggc agataccatg
     1141 aacgtaacca tgcggtggct aatcagcatc tgagcggtgg caatgatgta tgaaaaaaga
     1201 aagtaggcta ccaccgacgt tccgtggcag aaacggcgat gttccgtatc aaaaccttgc
     1261 tgggcggaca cttgagcctg cgagactacg atgcgcaggt ggggacgcgc aggtgggcga
     1321 agcgatggcg atggttaaag cgctaaaccg gatgacgttg ttgggtatgc cgcatagtgt
     1381 ccggatcgca taacgggtgt actgctggcc ccctcttcgg ctgactctga tttattcaac
     1441 aaagcctgtc aggagctgac tgccatgatg cttgtttttt tattcatctt attaagaacc
     1501 tatcccaaaa ggaattgtca gttaataagt aatgacaatt tctgagggaa gtatggcagg
     1561 taacaagtgg cagatcagcg atgaactctg ggaaaaaatg gctccgttgc tcccggaaca
     1621 caaaactcac catccgctgg gtacgcatcg caggcgggtt aataatcgtg ccgcaatgaa
     1681 cgccattctc tttgtgctca gaacgggttg ccagtggaat gccctgaatg ccaccgggat
     1741 atgctcgtca agctctgctc accgccgctt ccaggagtgg cgggatgcag gagtttttga
     1801 acggttctgg caaaacgggt tgctggcctg tgagcagttg gatgccatcg actggtcatg
     1861 gctgtcgatg gatggctgta tgacgaagtc accgctggct ggctcaaaaa acaggacgga
     1921 accccacaga acgagggaaa cagggcgtaa aacgtagcct gatgaccgat gcgaacgggc
     1981 ttcccctctc attggttgtc gcaggtgcaa atacccacga cataaagctg gttgctgata
     2041 ctctcgatgc ccttcagacg ggcaggcctg gcagaaggct ccacctctgt atggacaaag
     2101 gctatgaagc cgaatggctg gaggcctgtc tgaaaaaccg ccgttacgaa cctcatatcc
     2161 agtcaaggaa agaggagtcg gaagccatca aaagcacgga ttttaaagct caccgctggg
     2221 tcgtggagag aacgcacagt tggatgaatc gcttccgtcg ggtgttgact ctctgggaga
     2281 agaaagtcga aaattatgtg gcaatgctgt atcttacctg cggcgtcatt gtctggaata
     2341 aaatcctatt gggataggct ctaagattat tacactcaaa atacaacccg ttgtcttgag
     2401 taatactacg ctgcaatggg agtgttaggc ttagggtagt tgcagcggtc agtagcctgg
     2461 ccgtcaatac tcctgacaaa aaacaagagt ttcgcccttt ggcagccaga gccaagggcg
     2521 gtagcgtgcc tacattacat gtagtaagcc gtgattgacc atctcgcgag ttgttgagga
     2581 atggtatgag gatagtgtat tttgtcaatg cagcgtggta cttcgaactg cattggttgg
     2641 accggtcaga agcggcaatt agcaaagggt atgaagttca tcttgtaagt aattttgctg
     2701 atgacgccat caaaaataac ctggaaaaaa aaggtataaa atgttgggat attgagctta
     2761 acagattttc taaaaatgtg tttaaaaaca tttcaatatt cactgcattt agaaagatct
     2821 gtaagcaaat taaacccgat cttattcatc taattactat aaaaccaatc ctttttggtg
     2881 gattgtatgc ccgggttgca ggcatccctt ttgtcgtgag ctttgtggga ttagggagat
     2941 tgtttggcaa taaacgaggt tggttgaata aattcatttt caactcagta ctgtcaattt
     3001 atagtctttt gctatcagca aaaagcccag cgcaagtaat atttgagcat aatgctgact
     3061 ttgctgagct gagtaaatat attaaatttg atgaaaataa catccacatc attgaaggag
     3121 ccggggtaga caggtttcgc tttagttatc agccagaacc aactcagaat aatttctcgg
     3181 tattatttgc cagccgtttg ctatggtcaa aggggctcgg tgaagtagtc gaggccatcg
     3241 gtaaggttaa acaaaatgaa aataacacaa ccttatacgt tgcgggaatt ctcgatgaca
     3301 aagatccgga tagaattgaa ttagatcaaa ttttgcaatg ggaaaaagaa ggaaaaatca
     3361 tttggttagg tcgaagagat gacatccaaa atttaatcag aaatagccat gtcgtgattt
     3421 tgccaactaa atattcagaa ggcgt
//
LOCUS       DAJOWB010000253         3424 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJOWB010000253 DAJOWB010000000
VERSION     DAJOWB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.254"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            137..889
                     /locus_tag="OHO85_004825"
     CDS             137..889
                     /locus_tag="OHO85_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HCQ2674046.1"
                     /translation="MTTVNQISDANITSSLPPKIREAVEKVKAAKAVWQEERRKQTEA
                     AAMIETIRKRQEDTKTETQALNDEWRNLFRENQGNMTPRMKKLRAEIALGRETLDEFE
                     DLLAAQVAENEFLPWKTADAANHYISEHNRLIETHAVWLWNEFMKEHGQKLIQILGLL
                     KMTLGRSASAVIGVVHTVNDPESVLKQFISEQLTTPALSCNVSSTDDITLPGISIYAD
                     DKAIQDARQSPSPAARSRMLKQRDMVKGGEKK"
     gene            886..1440
                     /locus_tag="OHO85_004826"
     CDS             886..1440
                     /locus_tag="OHO85_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HCQ2674047.1"
                     /translation="MNAGTITQEALNDYRSAIKSWLTLRNTQSTYQLRLAALLNTEEK
                     PAAYASQLEELRERLAVLEWQINCAARDGLYAHQIVLESCVTSATENFMSEHGDALTD
                     ALAPFLCAPYGLEAAMKILRTAVARQTEVRTPVISAAYKSIIDETGLTVDASMYADAS
                     TSFTPAQHKVFLARLNRLNEKGGY"
     gene            1442..1714
                     /locus_tag="OHO85_004827"
     CDS             1442..1714
                     /locus_tag="OHO85_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCQ2674048.1"
                     /translation="MALKCPECGTVAHARTSAYEAPSVKRSWYQCQNLECSCTFTALE
                     SVDTIIMKPWRNEQESDKEQMPEKQQQTLNRYGSASKLSSRQLIPV"
     gene            2140..3081
                     /locus_tag="OHO85_004828"
     CDS             2140..3081
                     /locus_tag="OHO85_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1837 domain-containing protein"
                     /protein_id="HCQ2674049.1"
                     /translation="MDALHVALENLIRDDGQTLNSYFSEVKVNSLIENTNATCHFHCL
                     NLDGNGRPRVDALIQFLNDKVVDYAIPRKKINEAVQYFNETQSTAKITKLANQAKKLF
                     TRLANSGEGGEFILFLFAEQFLRFPQIICKMSLKTSSQMHYHGADGIHIGVDKDNKKL
                     CLYWGESKLYSNLSKGISECMDSIAPLLQSSMGIEGAETRDMQLLESHLNVDDIELEQ
                     ALKRFLDPDSPDFNKLEYRGICLVGFDLKDYPQGPSSIDISELVALINTKVGKWSTSV
                     QGGIVNKKLDRFAMHVFILPFPSVKAFRASLIKALES"
     gene            3083..>3424
                     /locus_tag="OHO85_004829"
     CDS             3083..>3424
                     /locus_tag="OHO85_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674050.1"
                     /translation="MSLRDLSGWLIKNQGFLRKYQALVANSIKDQFPELKSDVIPQIS
                     SEDVGYLLTCASSLAFSSDEKCQDAALRIAQYCLLNEKSEARKDSAALILDSLSNNPA
                     IQLAENRGYLNP"
BASE COUNT         1060 a          694 c          788 g          882 t
ORIGIN      
        1 aagatcagac atgacacatt tctctggcta gccagaacaa ggtcattgtt ctgtcactga
       61 cacaatcccc ctcggtagtg cgcttgtatg ggcatcggca caattgacac gacaacaaaa
      121 cactaccgga gcattcatga ctactgttaa ccagatctct gatgcaaaca ttacatcctc
      181 cctcccgccg aaaattcgcg aagcggtgga aaaagttaag gcagcaaaag ctgtctggca
      241 ggaagaacgg cgaaaacaaa ctgaagctgc tgcaatgatt gaaactatcc gtaaacgtca
      301 ggaagataca aaaacggaaa cgcaggcgct taatgatgaa tggcgaaacc tgtttcgtga
      361 gaatcaggga aatatgacac cacgcatgaa aaaactgcgg gcagaaatcg ccctcggacg
      421 cgaaacactg gatgagttcg aagatttgct ggcagctcag gtggcagaaa atgaattcct
      481 gccctggaaa actgcggatg ctgcaaacca ctacatcagc gaacataatc gcctgattga
      541 aactcatgca gtgtggctct ggaatgagtt tatgaaggaa cacggccaga aacttattca
      601 gatccttgga ctgttgaaaa tgactctggg ccgaagcgct tctgctgtta tcggtgtagt
      661 tcataccgta aacgaccccg aaagtgtgct gaagcaattt atcagcgagc aactcaccac
      721 tccggcactg tcctgtaacg tatcttcaac ggatgatatt accctgccgg ggatcagcat
      781 ttatgcggac gataaagcca tccaggatgc cagacaatcc cccagccctg cagcgcgttc
      841 acggatgctt aaacagcgtg acatggttaa agggggcgag aagaaatgaa tgccggaacc
      901 attactcagg aagctctcaa cgattaccgc tcggcgataa aaagctggct gacactacgt
      961 aatacgcaaa gtacctacca gcttcgtctg gcggctctgc tgaatactga agaaaaaccg
     1021 gcagcatatg ccagccagct tgaggagctt cgtgagcgtc ttgctgtcct cgaatggcag
     1081 attaactgtg ctgcacggga tggtctttat gctcaccaga ttgtgctgga gagttgtgtt
     1141 acaagcgcaa cggaaaactt catgagcgag catggtgatg cactcactga cgcactggct
     1201 ccttttcttt gcgctccata cgggcttgag gcggcaatga aaatattacg aaccgctgta
     1261 gcccgacaaa cggaagtgcg tactccggta atttcagcag catacaagag cattatcgac
     1321 gaaaccggat taacggtgga tgcatcaatg tacgctgatg cttcaaccag tttcaccccg
     1381 gcacaacata aagttttcct ggcccgcctt aaccgactta atgaaaaagg agggtactga
     1441 tatggccctg aagtgtcctg aatgtgggac ggtcgctcac gccagaacca gcgcctatga
     1501 agctccatcg gttaaacgct catggtatca gtgccagaat ctggaatgtt cctgcacatt
     1561 taccgctctg gaaagcgtgg atacgataat tatgaagcct tggcgcaatg aacaggaatc
     1621 agacaaagaa caaatgccag aaaaacaaca gcaaactctc aaccgctatg gctccgcgtc
     1681 aaagctgtca agccgtcagc taattcctgt ctgattagca aaatacgccc acgtagtccc
     1741 ggtccagtac cgggattttt tacgcctttt ccctggctgg cctgaaggcg tatgagtgca
     1801 tatctatagc gcatgaaaac gcatgagtct catgcgccat ttttgacgcg aaagcccttg
     1861 tgtggtggct tctgagacga tttacggggt gcatgaaaac cagtctgtta agcgaagcgg
     1921 gcaggcgggc ggggcagcgc acgctgtgta tggtcaaagt aacttttaac tttcaatgag
     1981 tacctgattg aaagaatcgt ttatcacgtt aaagcgataa caatgatttt aagcagcgag
     2041 tgtggtgtaa gtatcattgt cgagtaaaaa tgttgcaagc tattgaaata agtatatatt
     2101 gcaaatgatg aggaaaataa aatatgcaag gacctactca tggatgcact gcatgtcgcg
     2161 cttgaaaatc tgattagaga tgatggtcaa acactaaata gttattttag tgaagttaaa
     2221 gttaattcat tgattgaaaa tacaaatgca acttgtcatt ttcattgcct taatcttgat
     2281 ggcaatggac gtcctcgggt agacgcgtta atccaattct tgaacgataa agtagttgat
     2341 tatgctattc caaggaaaaa aatcaatgag gcagtgcaat attttaatga aacacaatca
     2401 acagcgaaaa taacaaaatt agcaaatcaa gcaaaaaaat tatttactag gcttgcaaat
     2461 agtggcgaag gcggtgaatt tattcttttt ttatttgcag agcaattcct tagatttcca
     2521 caaattattt gcaaaatgag cctgaaaacg agttctcaaa tgcattacca tggtgcagat
     2581 ggaatacata taggcgtaga taaagataat aaaaaattat gtttatactg gggcgagtct
     2641 aaactctatt ccaatctgag caaaggtatt tctgagtgta tggatagtat agctccatta
     2701 cttcaaagtt ctatgggaat agaaggcgct gagacacgtg atatgcagtt gctggagtcg
     2761 cacttaaatg ttgatgatat agaactagaa caagcgttga aaagattctt agatcctgat
     2821 agcccggatt ttaataaatt ggaatacaga ggaatatgct tagttgggtt tgatcttaaa
     2881 gattatccac aagggcccag ttcaattgat atcagtgaac tagttgcttt gattaatact
     2941 aaagtcggga aatggtcaac tagtgtgcaa ggtggtatag ttaataaaaa acttgatcgc
     3001 tttgctatgc atgtttttat tcttcctttt ccttcagtaa aagcatttag ggctagttta
     3061 attaaagcat tggagagttg aaatgtcgtt gcgagattta tctgggtggt tgattaagaa
     3121 tcaaggattc ttaagaaaat atcaggcatt agtcgctaac tcgataaaag atcaatttcc
     3181 agagttaaaa tcagatgtaa tcccacagat tagttctgag gatgtcggct atcttttaac
     3241 atgtgctagt tctttggctt tctcaagtga tgagaaatgc caagatgcag ccttaaggat
     3301 tgcacagtat tgcttattga atgaaaaaag tgaagcaaga aaagatagtg ctgcattaat
     3361 tcttgattca ttatctaata atccagcaat acaacttgct gaaaataggg ggtatcttaa
     3421 ccct
//
LOCUS       DAJOWB010000254         3410 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJOWB010000254 DAJOWB010000000
VERSION     DAJOWB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.255"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            120..1085
                     /gene="dusB"
                     /locus_tag="OHO85_004830"
     CDS             120..1085
                     /gene="dusB"
                     /locus_tag="OHO85_004830"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCQ2674051.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1111..1407
                     /gene="fis"
                     /locus_tag="OHO85_004831"
     CDS             1111..1407
                     /gene="fis"
                     /locus_tag="OHO85_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCQ2674052.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1493..2377
                     /gene="yhdJ"
                     /locus_tag="OHO85_004832"
     CDS             1493..2377
                     /gene="yhdJ"
                     /locus_tag="OHO85_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCQ2674053.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NRSKRCRVSEVDPDLIAK"
     gene            2461..2640
                     /locus_tag="OHO85_004833"
     CDS             2461..2640
                     /locus_tag="OHO85_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCQ2674054.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(2643..3305)
                     /gene="acrS"
                     /locus_tag="OHO85_004834"
     CDS             complement(2643..3305)
                     /gene="acrS"
                     /locus_tag="OHO85_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCQ2674055.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT          934 a          783 c          810 g          883 t
ORIGIN      
        1 ttttaaatgc aattctttga tccatctcag aggattggtc aaagtttggc ctttcatctc
       61 gtgcaaaaaa tgcgtaatat acgccgcctt gcagtcacag tatggtcatt tcttaactca
      121 tgcgcatcgg acaatatcag ctcagaaatc gcctgatcgc agcgcccatg gctggcatta
      181 cagacagacc ttttcggacg ttgtgctacg agatgggagc cggattgaca gtatccgaga
      241 tgatgtcttc taacccacag gtttgggaaa gcgacaaatc tcgtttacgg atggtgcaca
      301 ttgatgaacc cggtattcgc accgtgcaaa ttgctggtag cgatccgaaa gaaatggcag
      361 atgcagcacg tattaacgtg gaaagcggtg cccagattat tgatatcaat atgggttgcc
      421 cggctaaaaa agtgaatcgc aagctcgcag gttcagccct tttgcagtac ccggatgtcg
      481 ttaaatcgat ccttaccgag gtcgtcaatg cagtggacgt tcctgttacc ctgaagattc
      541 gcaccggctg ggcaccggaa caccgtaact gcgaagagat tgcccaactg gctgaagact
      601 gtggcattca ggctctgacc attcatggcc gtacacgcgc ctgtttgttc aatggagaag
      661 ctgagtacga cagtattcgg gcagttaagc agaaagtttc cattccggtt atcgcgaatg
      721 gcgacattac tgacccgctt aaagccagag ctgtgctcga ctatacaggg gcggatgccc
      781 tgatgatagg ccgcgcagct cagggaagac cctggatctt tcgggaaatc cagcattatc
      841 tggacactgg ggagttgctg cccccgctgc ctttggcaga ggttaagcgc ttgctttgcg
      901 cgcacgttcg ggaactgcat gacttttatg gtccggcaaa agggtaccga attgcacgta
      961 aacacgtttc ctggtatctc caggaacacg ctccaaatga ccagtttcgg cgcacattca
     1021 acgccattga ggatgccagc gaacagctgg aggcgttgga ggcatacttc gaaaattttg
     1081 cgtaaacaga aataaagagc tgacagaact atgttcgaac aacgcgtaaa ttctgacgta
     1141 ctgaccgttt ctaccgttaa ctctcaggat caggtaaccc aaaaacccct gcgtgactcg
     1201 gttaaacagg cactgaagaa ctattttgct caactgaatg gtcaggatgt gaatgacctc
     1261 tatgagctgg tactggctga agtagaacag cccctgttgg acatggtgat gcaatacacc
     1321 cgtggtaacc agacccgtgc tgcgctgatg atgggcatca accgtggtac gctgcgtaaa
     1381 aaattgaaaa aatacggcat gaactaattc aggttagcta aatgcttgat taaaaaggcg
     1441 ctactcggca tggggaagcg ccttttttat aggtgtcaca aagggagtga ccatgagaac
     1501 aggatgtgaa ccgacccggt ttggtaatga agctaagacc attattcacg gtgatgccct
     1561 tgccgaactt aaaaagctac ctactgaaag cgtcgatctg atctttgccg acccaccgta
     1621 taacatcggt aaaaattttg atggtctgat cgaagcctgg aaagaagatc tgtttatcga
     1681 ctggctgttt gaagtgattg cagagtgcca ccgcgttctg aaaaagcagg gcagcatgta
     1741 cattatgaac agtacggaaa acatgccctt tatcgatctc cagtgccgca agctttttac
     1801 catcaaaagt cgcatcgtct ggtcatatga cagttctgga gtacaggcga aaaaacacta
     1861 cggctccatg tacgaaccca tcctgatgat ggtgaaagac gcaaagaact acacattcaa
     1921 cggtgatgct attctggtag aagccaaaac cggatcgcag cgcgcgttga tcgattatcg
     1981 caaaaatcct ccacagccat acaatcatca aaaagtaccg ggtaacgtct gggattttcc
     2041 gcgcgtgcgt tatttaatgg atgaatatga gaaccacccg acgcaaaaac cggaagcctt
     2101 actgaaacgc attattctcg cctcttccaa cccaggcgat atcgttctcg acccgtttgc
     2161 cggtagcttt actaccggtg ccgtagccat cgccagcgga cgaaaattca ttggtattga
     2221 gatcaacagc gagtacatca aaatggggct tcgacggctg gatgtcgcgt cgcattactc
     2281 cgcggaagaa ctggcgaaag tgaaaaaaag aaagacgggc aaccggtcaa aacgatgccg
     2341 ggttagcgaa gttgaccccg atctcattgc aaagtaaagg aagtgtaagc ctggttttca
     2401 gattattcat ttcgtgtata tttcctgcca gacttggtta aacatgcaca ggctcaggta
     2461 atgattcgca agtattggtg gctcgtcgtt ttcgctgtct tcgtttttct gtttgatact
     2521 ttactgatgc agtggattga actgctggca acagaaacag acaaatgccg caatatgaac
     2581 tcagttaatc cactaaaact ggttaactgt gacgaactga attttcagga cagaatgtga
     2641 atttacatga cacttaattc attcgtttga tgaattaatt tcgttatgtt ttcatctggc
     2701 atgaacattc ttaatacgtt atcgaccagc gcgggtgctt gtttataaag atcataaccc
     2761 gccatattca ttaaccagtt ttgaacaatt ccgctgaagg caccatcaat aataatcatc
     2821 acaacatcta aatcgaggtt atttgctaca caaccttgtt gctgacacgc ctgcaatact
     2881 tcgcggagag tctgcggatt aaagcccatc ttttcgcgta tcactccctc ggccagcatc
     2941 tcatcattaa attcacattt gtgatataag attttcagca acgcctgctg gcggggaatt
     3001 ttggcaatat attgcaagcc gacaatcaat ttttcacgca attgttgaaa cgggtcatgc
     3061 tctaatccag ccgtcaagtg ttcctggatt aactcccgca atgaaggctg ttgcaaccac
     3121 atctcattaa acagttgagt cttgttttcg aagtgccagt agatagcgcc acgcgtaacg
     3181 ttagcagcgt cggcaatgtc gttgagtgtc gtcttgctta cgccatgctg cgcaaactgg
     3241 gcgatggcag tttcaatcag ttcttgccgg gtcttcagag cttcggcttt ggttcttttt
     3301 gccatgatta attattcagg aaataaatat attcgacaca gagtgagaaa atagcgaagg
     3361 ttaatctatc acctaatgtg tatttatacg agaggctaat attgagttgc
//
LOCUS       DAJOWB010000255         3360 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJOWB010000255 DAJOWB010000000
VERSION     DAJOWB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.256"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(18..170)
                     /gene="mokC"
                     /locus_tag="OHO85_004835"
     CDS             complement(18..170)
                     /gene="mokC"
                     /locus_tag="OHO85_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCQ2674056.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            756..1922
                     /gene="nhaA"
                     /locus_tag="OHO85_004836"
     CDS             756..1922
                     /gene="nhaA"
                     /locus_tag="OHO85_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCQ2674057.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1988..2887
                     /gene="nhaR"
                     /locus_tag="OHO85_004837"
     CDS             1988..2887
                     /gene="nhaR"
                     /locus_tag="OHO85_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCQ2674058.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2926..>3360)
                     /locus_tag="OHO85_004838"
     CDS             complement(2926..>3360)
                     /locus_tag="OHO85_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCQ2674059.1"
                     /translation="VNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQGE
                     LVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSNY
                     YSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          797 a          763 c          809 g          991 t
ORIGIN      
        1 actccccgcc gttgctctta ctcggattcg taagccgtga aaacagcaac ctccgtctgg
       61 ccagttcgga tgtgaacctc acagaggtct tttctcgtta ccagcgccgc cactacggcg
      121 gtgatacaga tgacgatcag ggcgacaatc atcgccttat gctgcttcat tgctctcttc
      181 tccttgacct tacggtcagt aagaggcact ctacatgtgt tcagcatata gggggcctcg
      241 ggttgatggt aaaatatcac tcggggcttt tctctatctg ccgttcagct aatgcctgag
      301 acagacagcc tcaagcaccc gccgctatta tatcgctctc tttaacccat tctgttttat
      361 cgattctaat cctgaagacg cctcgcattt ttatggcgta attttttaat gatttaatta
      421 tttaacttta atttatctct tcatcgcaat tattgacgac aagctggatt atttttgaaa
      481 tattggccta acaaacatcg ccgactgaca acaaattaat tattactttt cctaattaat
      541 ctctcagaaa tcctcacctt aagctatgat tatctgggct tagggtcact cgtgagcgct
      601 tacagccgtc aaaaactcat ctcaccgctg atggcgcaaa ttcttcaatg gatcgtaaaa
      661 aacgagttat tcctacacta taatctgatt ttaacgatga tttgtgcggg gtaaaatagt
      721 aaaaacgatc tattcacctg aaagagaaat aaaaagtgaa acatctgcat cgattcttta
      781 gcagtgatgc ctcgggaggc attattctta tcattgccgc tatcctggcg atgattatgg
      841 ccaatagcgg cgcaaccagt ggatggtatc acgactttct ggagacgccg gttcagctcc
      901 gggttggttc actcgaaatc aacaaaaaca tgctgttatg gataaatgac gcgctgatgg
      961 cggtattttt cctgttagtc ggtctggaag ttaaacgtga actgatgcaa ggatcgctag
     1021 ccagcttacg ccaggccgca tttccagtta tcgccgctat tggtgggatg attgttccgg
     1081 cattactcta tctggccttt aactatgccg atccgattac ccgcgaaggg tgggcgatcc
     1141 cggcggctac tgacattgcc tttgcacttg gtgtgctggc gctgttggga agtcgtgttc
     1201 cgttagcgct gaagatcttt ttgatggctc tggctattat cgacgatctt ggggccatca
     1261 ttatcatcgc attgttctac actaatgact tatcgatggc ctctcttggc gtcgcggctg
     1321 tagcaattgc ggtactcgcg gtattgaatc tgtgtggtgt acgccgcacg ggcgtctata
     1381 ttctggttgg cgtagtgttg tggactgcgg tgttgaaatc gggggttcac gcaaccctgg
     1441 cgggggtaat tgtcggcttc tttattcctt tgaaagagaa gcatgggcgt tctccagcga
     1501 agcgactgga gcatgtgtta cacccgtggg tggcgtatct gattttgccg ctgtttgcat
     1561 ttgctaatgc tggcgtttca ctgcaaggtg tcacgctgga tggcttgacc tccattctgc
     1621 cattggggat catcgctggc ttgctgattg gcaaaccgct ggggattagt ctgttctgct
     1681 ggttggcgct gcgtttgaaa ctggcgcatc tgcctgaggg aacgacttat cagcaaatta
     1741 tggcggtggg gatcctgtgc ggtatcggtt ttactatgtc tatctttatt gccagcctgg
     1801 cctttggtag cgtagatcca gaactgatta actgggcgaa actcggtatc ctggtcggtt
     1861 ctatctcttc ggcggtaatt ggatacagct ggttacgcgt tcgtttgcgt ccatcagttt
     1921 gacaggacgg tttaccgggg agccataaac ggctcccttt tcattgttat cagggagaga
     1981 aatgagcatg tctcatatca attacaacca cttgtattac ttctggcatg tctataaaga
     2041 aggttccgtg gttggcgcag cggaggcgct ttatttaact ccacaaacca ttaccggaca
     2101 gattcgagcg ctggaagagc gcctgcaagg caaattattt aaacgcaagg gacgtggtct
     2161 cgaacccagc gagctgggag aactggtcta tcgctatgcc gataaaatgt tcaccttaag
     2221 ccaggaaatg ctggatattg tgaactatcg caaagaatcc aatttattgt ttgacgttgg
     2281 tgtggctgat gcactttcca aacgcctggt cagtagcgta cttaacgccg cagtggtaga
     2341 aggcgagccc attcatcttc gctgcttcga atccacccac gaaatgctgc tggagcaatt
     2401 aagtcagcat aaactggata tgatcatttc tgactgtccg atagactcta cgcagcagga
     2461 aggcctgttc tccgtgagaa ttggcgaatg tggcgtgagt ttctggtgta caaatccacc
     2521 accagaaaaa ccgttcccgg cttgtctgga agaacggcga cttttgattc ctgggcgacg
     2581 ttcaatgtta gggcgcaaat tgcttaactg gtttaactcc cagggattaa acgtagaaat
     2641 cctcggcgag tttgatgatg ccgcgttgat gaaagctttt ggtgcgatgc acaatgcaat
     2701 cttcgttgcc ccaacgcttt atgcatatga cttttatgcc gataaaactg tcgtagaaat
     2761 tggtcgcgtc gagaatgtga tggaagagta ccatgctatt tttgctgagc ggatgattca
     2821 gcacccggcg gtacagcgaa tctgcaatac ggattattct gcgcttttta gtccagcggc
     2881 gcgttaatcg gcacctcccc ccaaagttaa ggtgggggag atagattagt tgtacataac
     2941 cacgattttg actcggctca ttatttgccc gcttgagaca ttatttccat atgtacgcgg
     3001 gcgaataaat agaggaatct gattacttcc ttcatgggga tgctgaaaag agtagtaatt
     3061 gcttttacca tactggattt cattcgtacc atcattggag agttgaaagc ctatttgacc
     3121 cgcaccgtcc tcactgtttt ctaactcatt ttttaatatg ctggttcctg actcctgtat
     3181 cgtcactggt tcaaagtaat attcaacatc actgggttca aaaactaatt caccttgctc
     3241 gccctggatg cagtcaaatc ttatatgatg aaactgataa ttttcattat tgatacgaga
     3301 cagtacaaat cgtccagtgt cgatggattg atcttcaaaa ccacaggtca ttaaatttac
//
LOCUS       DAJOWB010000256         3331 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJOWB010000256 DAJOWB010000000
VERSION     DAJOWB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.257"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..2292)
                     /gene="nikB"
                     /locus_tag="OHO85_004839"
     CDS             complement(<1..2292)
                     /gene="nikB"
                     /locus_tag="OHO85_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type relaxase NikB"
                     /protein_id="HCQ2674060.1"
                     /translation="MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQ
                     PHRSRFSRLVDYATRLRNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEY
                     IARQAHYAKDDTDPVFHYILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTD
                     TDNLHVHVAVNRVHPETGYLNRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRI
                     VRKTAVERDRQNAWTRGKKQTFREYVAQTAVAGLRSEPVHDWLSLHRRLAEDGLYLSQ
                     MDGKFLVMDGWDRNREGVQLDSFGPSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNP
                     DFIAADVRPEKIAETESLQQYACRHLGERLPEMAREGRLENCLAIHRTLAEAGLWMRV
                     QHGHLVICDGYDHNQTPVRADSVWSLLTLDNVNQLDGGWQPVPTDIFRQVTPTERFRG
                     RRMESCPATDKEWHRMRTGTGPQGAIKRELFSDKESLWGYSISHCSPQIEEMITQGEF
                     TWQRCHELFAQQGLMLQKQHHGLVVVDAFNHEQTPVKASSIHPDLTLGRAEPQAGPFV
                     SAPADLFDRVQPENRYNPELAVSDRYGVSSKRDPMLRRQRREARAEARADLRARYLAW
                     REQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDPALRKLHYHIAEVQRMQALIRLK
                     EDIRDERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVSQLRGWDYRDRRKDKSRTTTT
                     DRCVVLCEPGGTPVYGNTGDLEAR"
     gene            complement(2303..2635)
                     /locus_tag="OHO85_004840"
     CDS             complement(2303..2635)
                     /locus_tag="OHO85_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCQ2674061.1"
                     /translation="MSDKRQRSGSERRQKTILRAVRFSPDEDETICKNAEDSGLTVSA
                     YIRNAALNKRINSRTDDAFLKELMRLGRMQKHLFVQGKRTGDKEYAEVLVAITELTNT
                     LRKQLMEG"
     gene            2868..3203
                     /locus_tag="OHO85_004841"
     CDS             2868..3203
                     /locus_tag="OHO85_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HCQ2674062.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
BASE COUNT          821 a          881 c          779 g          850 t
ORIGIN      
        1 acgggcctcg agatcaccgg tattaccgta taccggcgtt ccgcccggtt cacaaagcac
       61 gacacagcgg tctgtcgtcg ttgtgcgtga cttgtcttta cggcgatcgc gataatccca
      121 gcctctcaac tgcgatacag cagccctgtc tccctgagca gcctgaattt cgacccactg
      181 acggtaagag ggtggatacc atttcccgtc agcaataagt ttctgtcgct catccctgat
      241 gtcttctttc agcctgatca atgcctgcat tcgctgaact tctgcaatgt gataatgcag
      301 cttacgtaat gccggatcat cgtactgcgc acgaatgtgt gacttccgca gacggcatgc
      361 ctgatgaatt tcccgacaac gttccccata acgcagatcc ggtttacgcc attgttcacg
      421 ccatgccaga tagcgggcac gcaggtcagc acgggcctca gccctggctt cacgtcgctg
      481 ccggcgcagc attgggtcac gtttactgct gaccccgtac ctgtcactga cagccagctc
      541 cgggttatag cgattttcag gttgcaccct gtcaaacaaa tctgctggag cactcacaaa
      601 aggtccggcc tgtggctccg cacgccccag tgtcagatca ggatgaatgc tgctggcttt
      661 caccggcgtt tgctcatgat taaaagcatc aacgacaaca agcccgtgat gttgtttctg
      721 tagcatcagt ccctgttgtg caaacaactc atgacaacgc tgccaggtaa actcaccctg
      781 tgtgatcatt tcttcaatct gagggctaca atggctgatg ctgtatcccc acagactttc
      841 tttgtcagaa aacagttcgc gtttgatagc cccctgcggc cccgtacctg tacgcatacg
      901 gtgccattct ttatcggtcg caggacagct ctccatacga cgaccacgga agcgttctgt
      961 tggcgtaacc tggcggaaaa tatctgttgg tacaggctgc cagccaccat cgagttgatt
     1021 cacattgtcg agcgtcagca gtgaccatac gctgtcagca cgaacgggag tctggttatg
     1081 atcataaccg tcacagataa ccaggtgacc atgctgaaca cgcatccata atcccgcttc
     1141 agccagtgtg cggtgaatag ccaggcagtt ttccagccgt ccttcccgtg ccatttccgg
     1201 cagacgttct ccaagatgac gacaggcata ctgctgcaga ctttctgttt cagcgatttt
     1261 ttccgggcga acatcagctg caatgaagtc cggattatag cgtcctggtg cttccacctg
     1321 gctgaaaatg tcttttggca ctggcgtgta gtcacccatt tttttcatga gtttttctgc
     1381 acaccaggag ggaccaaacg agtcaagctg tacaccttcc ctgttgcgat cccagccatc
     1441 catcaccaga aattttccgt ccatctgaga caggtacaga ccatcttccg caagacgacg
     1501 atgcagggat aaccagtcat gtacaggttc actgcgtaaa ccagcgaccg ctgtctgcgc
     1561 aacatactca cgaaaagttt gtttttttcc gcgtgtccag gcgttctggc gatcacgttc
     1621 aacggcagtt ttgcgaacga tacgatttcc cggcgcatgg acccagcaac cgttatccgg
     1681 agcaaaacca tgcttcagtt caagctcacg gcaggcacga ctgagttttt cctgactcca
     1741 tgataaccgg ttcagataac ccgtttcagg gtgtacccgg ttaacagcca catggacgtg
     1801 cagattatct gtatcagtat gcacggcaga gacatactga tggtcggcaa ggccgagtga
     1861 tttcagcgta tgacgtacac tgtcataaat ctgttccgga cgcgggcttt catgtgactg
     1921 ccaggaaagg atgtagtgaa aaacagggtc agtgtcatct tttgcatagt gtgcctgccg
     1981 ggcaatgtac tccatatctg cagcggcagt ttcaagagaa gtacagttat gaaagcaggt
     2041 gacaccataa aagttgaccc attcgcagcc gtccttcatg acatcaacca gcgccacgaa
     2101 tgactcattt cgaagacgtg tcgcataatc aacaaggcga ctgaaacggc ttcggtgcgg
     2161 ttgttcagcc tgcgaagaag aagataaatc cagttcctca tcggtcatgt catcccggac
     2221 agagacgtag gataccaggt cttcgaacga agacttgccg tcccttctct ttttcggaat
     2281 gactgcattc accggagaat cctcaacctt ccattaactg tttacgcaat gtatttgtaa
     2341 gttcggttat ggcaacaagc acttctgcgt attccttgtc accggttctt tttccctgaa
     2401 caaacagatg cttctgcatc cttcccagtc tcattaactc cttcagaaaa gcatcatctg
     2461 tacgggagtt aattcgctta ttcagtgctg catttcggat gtaagcagat acagtaagtc
     2521 cggaatcttc agcattctta cagatagttt catcctcatc cggtgagaac cggaccgctc
     2581 tcaggatcgt cttttgccgt ctttcactac cacttctttg acgtttatca ctcacggtca
     2641 gctccttact ggggtgtcgg ggcgaagccc tgaccaggag gcaattgtat aatcgcgcgt
     2701 gcgcggttat acaattgcac atcctgtccc gtttttcagg ccattatagc ctgttatttt
     2761 tgtgcatcat agtcttatgc agacggcaga ggcagggcgc agccctgcca ggagtcagtt
     2821 tttgatgata aaatcagact tcaacatgaa acggagagaa agtgccaatg gcttctaaaa
     2881 aattttactc agatgatgac atccagctgg caaaagctgc tttatcggaa ttgccagatc
     2941 tcaccgctca acggaaaaca ttgcgtgatt ttcttgatgc cattcgtgat gacattattg
     3001 tcctggtaag gacaaagggt tatacactgg cagatgttcg tgacacgctc aaaaatgcag
     3061 ggtatgaagt aggagagaag gcacttcgtg acattattcg ggaagcagaa tcgaaaaaac
     3121 catcccgccg ttcttcctct aaaacagcac ctaaaaagac agattcaggc aggaaagatg
     3181 gcatcgatat gaacaacaat tgatgtgaca atgtcacatc gtgttctgag attatgatga
     3241 ttacagaaaa aaaagccccg gcataaatac cagggctttt ctgtttaatc agactcgtgg
     3301 cggcaatgca cctggataat gccgtgcaat a
//
LOCUS       DAJOWB010000257         3313 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJOWB010000257 DAJOWB010000000
VERSION     DAJOWB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.258"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..70
                     /gene="cvi"
                     /locus_tag="OHO85_004842"
     CDS             <1..70
                     /gene="cvi"
                     /locus_tag="OHO85_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colicin V immunity protein"
                     /protein_id="HCQ2674063.1"
                     /translation="GDNYYSISDKIKRRSYENSDSK"
     gene            48..359
                     /gene="cvaC"
                     /locus_tag="OHO85_004843"
     CDS             48..359
                     /gene="cvaC"
                     /locus_tag="OHO85_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V"
                     /protein_id="HCQ2674064.1"
                     /translation="MRTLTLNELDSVSGGASGRDIAMAIGTLSGQFVAGGIGAAAGGV
                     AGGAIYDYASTHKPNPAMSPSGLGGTIKQKPEGIPSEAWNYAAGRLCNWSPNNLSDVC
                     L"
     gene            complement(529..2643)
                     /locus_tag="OHO85_004844"
     CDS             complement(529..2643)
                     /locus_tag="OHO85_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HCQ2674065.1"
                     /translation="MQQDRSMTNRNFRQIINLLDLRWQRRVPVIHQTETAECGLACLA
                     MICGHFGKNIDLIYLRRKFNLSARGATLAGINGIAEQLGMATRALSLELDELRVLKTP
                     CILHWDFSHFVVLVSVKRNRYVLHDPARGIRYISREEMSRYFTGVALEVWPGSEFQSE
                     TLQTRISLRSLINSIYGIKRTLAKIFCLSVVIEAINLLMPVGTQLVMDHAIPAGDRGL
                     LTLISAALMFFILLKAATSTLRAWSSLVMSTLINVQWQSGLFDHLLRLPLAFFERRKL
                     GDIQSRFDSLDTLRATFTTSVIGFIMDSIMVVGVCVMMLLYGGYLTWIVLCFTTIYIF
                     IRLVTYGNYRQISEECLVREARAASYFMETLYGIATVKIQGMVGIRGAHWLNMKIDAI
                     NSGIKLTRMDLLFGGINTFVTACDQIVILWLGAGLVIDNQMTIGMFVAFSSFRGQFSE
                     RVASLTSFLLQLRIMSLHNERIADIALHEKEEKKPEIEIIADMGPISLETNGLSYRYD
                     SQSAPIFSALSLSVAPGESVAITGASGAGKTTLMKVLCGLFEPDSGRVLINGIDIRQI
                     GINNYHRMIACVMQDDRLFSGSIRENICGFAEEMDEEWMVECARASHIHDVIMNMPMG
                     YETLIGELGEGLSGGQKQRIFIARALYRKPGILFMDEATSALDSESEHFVNVAIKNMN
                     ITRVIIAHRETTLRTVDRVISI"
     gene            complement(2618..>3313)
                     /locus_tag="OHO85_004845"
     CDS             complement(2618..>3313)
                     /locus_tag="OHO85_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCQ2674066.1"
                     /translation="ALYYQQQNNLLSLSGQNEQNALQITTLESQIQTQAADFDNRIYQ
                     MELQRLELQKELVNTDVEGEIIIRALSDGKVDSLSVTVGQMVNTGDSLLQVIPENIEN
                     YYLILWVPNDAVPYISAGDKVNIRYEAFPSEKFGQFSATVKTISRTPASTQEMLTYKG
                     APQNTPGASVPWYKVIATPEKQIIRYDEKYLPLENGMKAESTLFLEKRRIYQWMLSPF
                     YDMKHSATGPIND"
BASE COUNT          942 a          762 c          664 g          945 t
ORIGIN      
        1 tggtgataat tattattcaa tatccgataa gataaaaagg agatcatatg agaactctga
       61 ctctaaatga attagattct gtttctggtg gtgcttcagg gcgtgatatt gcgatggcta
      121 taggaacact atccgggcaa tttgttgcag gaggaattgg agcagctgct gggggtgtgg
      181 ctggaggtgc aatatatgac tatgcatcca ctcacaaacc taatcctgca atgtctccat
      241 ccggtttagg gggaacaatt aagcaaaaac ccgaagggat accttcagaa gcatggaact
      301 atgctgcggg aagattgtgt aattggagtc caaataatct tagtgatgtt tgtttataaa
      361 tagtattatt taatataaga aagaacagtt attggacaat ccacacagaa tgatatttct
      421 tcttggtgga ttgtctatct tccattccac ctccaacaga tatctgagca taacgaggaa
      481 ttataacagg aagaaatatt cctcatacgc ttgtaattcc tctatggttt aaatagaaat
      541 aactctatca acagttctca acgttgtttc tctgtgtgca ataattaccc tggtgatatt
      601 catgtttttt atggcaacat tcacgaaatg ttcactctct gaatcaagag cactggttgc
      661 ctcatccata aataatattc ctggtttccg gtataaggct cgtgcaataa atatacgctg
      721 tttttgaccg ccagaaagac cttccccaag ttcacctatt aatgtttcat atcccattgg
      781 catattcatt ataacatcat gaatatgact tgctctggca cattctacca tccactcttc
      841 atccatttcc tctgcaaaac cacagatatt ttcacgaatt gagcctgaaa atagccggtc
      901 atcctgcata acacaggcta tcatccggtg ataattattt attccaattt ggcgtatatc
      961 tataccattt atcagtaccc tcccgctatc aggttcaaat agtccacata gtactttcat
     1021 taatgtggtt tttcccgcac cggaagcacc agttatagcc acactttccc ccggagctac
     1081 agataaactc agagcactga atatcggtgc tgactgactg tcataacgat agcttaaacc
     1141 attggtttcc agggatattg gccccatatc agcaatgatt tcaatttcag gtttcttttc
     1201 ctccttttca tgtaatgcaa tatctgcaat gcgctcattg tgcagactca ttattcttag
     1261 ctgaagaaga aaactggtca gagaggcaac tctttccgaa aactgcccac gaaaagaact
     1321 aaacgctaca aacattccta ttgtcatctg attatcgatc acaaggcctg ctcccagcca
     1381 taaaattaca atctgatcac aggcggtaac aaaggtattt attcctccga agagcaaatc
     1441 catcctggtt agcttaatac ccgaatttat cgcatctatt ttcatattaa gccagtgtgc
     1501 cccccgaatt ccgaccatcc cctggatttt taccgtggca ataccatata atgtttccat
     1561 aaaataggag gcggcacggg cctccctgac aagacattct tctgatatct gtcggtaatt
     1621 gccgtatgtc accagtcgaa taaaaatgta aattgtggta aagcagagaa ctatccaggt
     1681 gagatatcct ccgtataaca gcatcatcac acaaacaccg acaaccataa tgctgtccat
     1741 tatgaacccg atcacactgg tggtaaatgt ggccctcaat gtgtcaaggg agtcaaaacg
     1801 tgactggata tcacctaatt ttcggcgttc aaaaaacgcc agcggtagtc tgagaagatg
     1861 atcgaacagc cccgactgcc actgtacatt gatgagcgtg ctcataacca gtgaagacca
     1921 tgcgcgcagc gtactcgttg cagctttgag taatataaaa aacataagag cagcagaaat
     1981 tagcgtcagt agccctctgt cccccgcagg aatagcatga tccataacca gctgtgtccc
     2041 caccggcatt agcagattga ttgcttcaat tacaactgac agacagaaaa ttttcgccag
     2101 cgttctttta ataccgtaaa tactgttaat cagtgaacga agacttatgc gggtctgcag
     2161 ggtttccgac tggaattcac ttccgggcca gacctcaagt gcaacgcctg taaaatatcg
     2221 gctcatttcc tcccggctga tatatcttat gcccctggcc ggatcatgca gtacataacg
     2281 gttacgcttt acgctgacca gaacgacgaa gtgactgaaa tcccagtgga gaatacacgg
     2341 cgttttgagg actcgaagtt catccaactc cagtgaaaga gcccgggtgg ccatccccag
     2401 ttgctccgct attccattga ttcctgcaag ggttgctcca cgggcagaga gattaaactt
     2461 ccggcgaaga tatatcaggt caatattctt accaaaatga ccgcatatca ttgctaggca
     2521 ggccagtcca cattcagcgg tctccgtctg atgaataacc ggaacacgac gttgccagcg
     2581 caaatcaagc agatttataa tttgtctgaa attcctgtta gtcattgatc ggtcctgttg
     2641 cactgtgttt catgtcatag aaaggagaaa gcatccactg gtaaatacgc cttttttcca
     2701 gaaatagtgt actttcggct ttcattccat tttccagagg gaggtatttt tcgtcatacc
     2761 tgattatctg cttttcaggc gtcgcaatga ctttatacca gggaacagag gcacccggcg
     2821 tattttgagg tgctccctta taggtcaaca tttcctgtgt tgacgcagga gtcctggata
     2881 tagttttaac cgtagcagag aactgcccaa atttttctga ggggaaggct tcataacgaa
     2941 tattcacttt gtcaccagcc gaaatataag gaacagcatc attcgggacc cagagaataa
     3001 gataatagtt ttcaatgttc tcaggaataa cctgcagaag gctgtctccg gtattgacca
     3061 tttgccctac agtgacactc agggagtcaa ctttcccgtc agacaacgcc cggataatga
     3121 tttcgccttc cacatcagtg ttaaccagtt ctttctgcaa ttcgagtcgt tgcagttcca
     3181 tctgatagat acgattatca aaatctgctg cctgagtctg aatctgactc tccagagtgg
     3241 ttatctgcag ggcattttgt tcattttgtc cgctcagact gagaaggttg ttttgttgtt
     3301 gataatataa tgc
//
LOCUS       DAJOWB010000258         3305 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJOWB010000258 DAJOWB010000000
VERSION     DAJOWB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.259"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(41..499)
                     /locus_tag="OHO85_004846"
     CDS             complement(41..499)
                     /locus_tag="OHO85_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCQ2674067.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(554..1405)
                     /gene="manZ"
                     /locus_tag="OHO85_004847"
     CDS             complement(554..1405)
                     /gene="manZ"
                     /locus_tag="OHO85_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCQ2674068.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1418..2218)
                     /gene="manY"
                     /locus_tag="OHO85_004848"
     CDS             complement(1418..2218)
                     /gene="manY"
                     /locus_tag="OHO85_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCQ2674069.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2281..3252)
                     /gene="manX"
                     /locus_tag="OHO85_004849"
     CDS             complement(2281..3252)
                     /gene="manX"
                     /locus_tag="OHO85_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCQ2674070.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
BASE COUNT          877 a          848 c          793 g          787 t
ORIGIN      
        1 ttaaaaatac actaaacata gctttggcta aattcataac ttattgagtt gaaacgagaa
       61 gcttataaat tttttccaga tcgtcgatat ttcgaacgcg gattaacagc cgacgctgtt
      121 ctaattgcat caccaacacg ccatcttccg acaagttcat cgctttgatt cggctatatt
      181 caatccagac attggcgaag aaaaaacctt tttgtttaaa gatgatcttc ggaacgcgga
      241 tccagaatat ataaaaaccc atcagagcca gtgcgcttaa taaccatgtc gttattaacg
      301 caccatgatt cgtgacgttg ttatagataa gaatgacaat caatccgacg aagataacgc
      361 tatcgatgcg accacgccgg agcaaaggaa ttgccagcag ggtggggcca ttacggcggg
      421 gcatgatgaa ctgatcgtag atcgcgaagg ccaggagtgc ggcgatgaaa agaatcagta
      481 ccaggtccgt gattgtcatt aatcctccag ataaaaaaac ggggccaaaa ggccccggta
      541 gtgtacaaca gtcttacagt cccagcaggc cgcaagcgta accagcgata ccgatgacga
      601 agaagccaac gatgatccac agcgggttaa cttttttgcg cagtagccac atacaagcaa
      661 aggtcagcag cagtggtacc aggcctggca ttaactggtc cagaatagtc tggacagtag
      721 taacgtgttc tttgcccgtc tggtcagtaa tgcgagagac aaccagcggg atgttgacat
      781 gtgtccactt gttaaccaat gcccccatga caaacaggcc gaggatagac gccccttccg
      841 tcagtttttg caggaagcca ccacccatat ctttaacgat atcgatacct ttggagtaac
      901 catacgctac gccgtagtaa cgggttgcca gacgcaccag gttaaacagg atgaagaaca
      961 gcagcggacc taacaggctg ccgctcatcg cgataccggc acccagtgct gcaaataccg
     1021 gacgtacggt tccccagaag atcgggtcgc ctacaccagc cagcggcccc atcaaaccga
     1081 ctttgatacc gttgatagca ccgtcgtcga tctctgcgcc attagcacgc tgttcttcca
     1141 gcgccagggt tacgccgaga atcggcgcag ccacgaacgg ctgggtgtta aagaactcca
     1201 ggtgacggcg aatagcttgt ttacgagctt cgttgttctc agggtagagg cgacgaattg
     1261 ccggtaccat agagaagcag aaacccagtg cctgcatacg ttcgaagttc catgaaccct
     1321 ggaagaggtt agaacgcagg aagacgccac gaatatcact ttgagtgagt tttttctcgg
     1381 tggtagtttg agttgtatca accatttcgc tcacctgtta gtccagttcg ttatcgagat
     1441 cgttgttacc agctgcctga gcaggcgcac cggctacgcg gttgtatttc gggctaagtt
     1501 ggatgtagag cactgccata acagtaccaa tcacacccag agcaaccagg ttaaagttgg
     1561 tgaatgctgc ggttacgaag ccgaggtaga agaacggcat caggtagcca gcacgcatca
     1621 tgttgataac catcgcataa ccaaccacca cgatcatgcc accggcgata ttcagaccat
     1681 tggtcaccac ttccggaatc gcattcagca tgttctgtac ttcgctggta ccaacagaca
     1741 gcgcaacgat gacggccgga atagccacac gcattgcttg caggaacaga gaagaaacgt
     1801 ggatccagga aatcgctgtc aggttgccgt tatcagcagc cttatccgca gcgtgctgga
     1861 aagcaacggt aatagtacga acgatgatgg tcagtacctg gcccgcagcg gccagaggga
     1921 ttgccagtgc gatacctgca ccaatgctct gatgacctgc gataaccaga atagtagaaa
     1981 tgatggaagc cagagcggcg tcaggcgcaa ctgcagcacc gatgttcatc cagcccagcg
     2041 cgatcatttc cagcgtacca ccgataataa taccggtttt catatcgcca agaacgatac
     2101 ccaccagggt acacgcgatt agcggacggt gaaactgaaa ttcatcgagg attgatccca
     2161 tacctgcgat acaggctacg ataaatacca gcacaatttg aagagtggta atctccattg
     2221 tacttctcct gtttacgtaa gacttaagtg tgaaaactga gtgataatca acacaatacg
     2281 ttacttatcg attttgctga tcagatccat cattttcagt ttcggatcgg tggaaacctt
     2341 acggacttcc agctcaatac cgcgcgcatt cagtttcttg aacgcctcga tatctttttc
     2401 atcaaccgaa accgcgttat tcacctgggt tttaccctga cggaatgcca taccaccgac
     2461 gttaacagag gtgattttca cgccgccttc aacgagacgc tctacatctg ttgggttggt
     2521 aaataacagc attacacgtt cgccagcata tttcgggttg ttgtagacgc gaatcatttt
     2581 ggcaacatca actacgtgtg ctgttacgcc cggaggtgca acctgggtga gcagtgtctt
     2641 acgaacggta tccgcagcca cttcatcact aacaacaata atacgggaga cattggtttc
     2701 tttggtccag cgggtggcga cctgaccgtg aatcagacgg tcgtcgatac gcgcaaggcc
     2761 aataaccatg tagtcgtttg gccccattgg ttttgccgga gttggagccg cttttggtgc
     2821 tgcggcagcg ggtgcaggcg cggctttttc aaccggtttg gctttcagtg ctttcacgcc
     2881 ttcacggcct gtttctactg ccagcgcaac cagttcatca aagcttgggt catcatcacg
     2941 agccattaac gtttccacga gcattggaat gttaacgcct gcaatgactt cataatgctc
     3001 tttgtcgacg acaatgcggc tggcagcatt gaacgggctg cctccccatg tatcaacgag
     3061 aaacagcacg cctttagtgg tgtcgagttt tgccaactga gcgttgtact tttcaatcag
     3121 cgtttcggca ttttcacctg gaacgaaatc gatccagccg acgttttcct gctcgcctaa
     3181 cagcatttct gccgttttaa gcaactgctc tgcagcccaa ccatgtgtgc ctataacaat
     3241 agcaatggtc acttgctacc tcctttatta tcgttaacac ctcaacgtgc cagatgtatt
     3301 tttga
//
LOCUS       DAJOWB010000259         3252 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJOWB010000259 DAJOWB010000000
VERSION     DAJOWB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.260"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..320
                     /locus_tag="OHO85_004850"
     CDS             <1..320
                     /locus_tag="OHO85_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="catalyzes the formation of of fructose 6-phosphate
                     from mannose 6-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCQ2674071.1"
                     /translation="LVANVKFEAKPANQLLTQPVKQGAELDFSIPVDDFAFSLHDLSD
                     KETTISQQSAAILFCVEGDATLCQGSQQLQLKPGESAFIAANESPVTVKGHGRLARVY
                     NKL"
     gene            421..1929
                     /locus_tag="OHO85_004851"
     CDS             421..1929
                     /locus_tag="OHO85_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCQ2674072.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(1974..3239)
                     /gene="uidC"
                     /locus_tag="OHO85_004852"
     CDS             complement(1974..3239)
                     /gene="uidC"
                     /locus_tag="OHO85_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCQ2674073.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSALGDRLNYWDVKMSIQYDFMLK"
BASE COUNT          910 a          821 c          745 g          776 t
ORIGIN      
        1 aactggttgc caatgtgaag ttcgaagcca aaccggctaa ccagttgttg acccagccgg
       61 tgaaacaagg tgcagaactg gacttctcga ttcctgtgga tgattttgcc ttctcgctgc
      121 atgatcttag tgataaagaa accaccatta gccagcaaag tgccgccatt ttgttctgcg
      181 tcgaaggcga tgcaacgctg tgtcaaggtt ctcagcagtt acagcttaaa ccgggtgaat
      241 cagcgtttat tgccgccaac gaatcaccgg tgactgtcaa aggccacggc cgtttagcgc
      301 gtgtttacaa caagctgtaa gagcttactg aaaaaattaa catctcttgc taagcttatt
      361 aaaggcttat aacaccttca ggcggccagt ccgcctgatt tcattttatg gataatcatt
      421 atgaataaat cgctggtagc ggtaggcgtc attgttgcgc taggcgtagt ctggacaggc
      481 ggcgcatggt atacaggcaa gaagattgaa acccatctcg aagacatggt cgcgcaggcg
      541 aacgcgcaac tcaaactgac agctcctgaa tccaacctgg aagtgagtta tcaaaactat
      601 catcgcggcg tattcagcag ccagttgcaa ctgttggtga aacccattgc cgggaaagaa
      661 aatccgtgga ttaaaagcgg tcagagcgtc atcttcaacg aatcggttga tcatggtccc
      721 ttcccccttg cccagcttaa aaaactgaac ctgatcccgt cgatggcatc aattcaaacc
      781 acgctggtca ataacgaagt aagcaaacca ctgtttgata tggcaaaagg tgaaacgcct
      841 tttgagatta actcccgcat tggttacagc ggtgattcca gttccgatat ttcgctcaat
      901 ccactgaatt acgagcaaaa ggatgaaaaa gtcgccttta gcggcggcga gttccagtta
      961 aatgctgaca gagacggcaa agctatctcc ctttccgggg aagcgcaaag tggtcggata
     1021 gacgcagtta acgaatacaa tcagaaagtg cagttgacct ttaataatct gaaaaccgac
     1081 ggttccagca cgctggcaag ttttggtgag cgcgtaggaa accaaaaact gtcactggaa
     1141 aaaatgacca tttcagtgga aggcaaagaa ctggcactgc tggaaggcat ggagatcagc
     1201 ggtaaatcgg atctggtcaa tgatggtaaa acgatcaata gccaactgga ttactcgcta
     1261 aacagcctga aggtacagaa tcaggatctg ggcagcggca agctgacttt aaaagtcggc
     1321 caaattgatg gcgaagcctg gcatcagttt agccagcaat ataacgcgca aactcaggcg
     1381 ctgctggcac agccagaaat tgccaacaat cccgaacttt atcaggagaa agtgacggaa
     1441 gccttcttta gcgccctgcc gctgatgctg aaaggcgatc cggtgattac tattgcgccg
     1501 ctaagctgga aaaacagtca gggtgaaagt gcgctgaatc tgtcgctgtt cctgaaagat
     1561 ccggcaacga ctaaagaagc gccgcaaacg ctggcgcagg aagtagatcg ttcggttaaa
     1621 tctctggatg cgaaactgac cattccggtg gatatggcaa ctgagtttat gactcaggta
     1681 gcgaagctgg aaggttatca ggaagatcaa gcgaaaaaac tggcgaaaca gcaagttgaa
     1741 ggtgcatcag caatggggca gatgttccgt ctgaccacct tgcaggacaa taccatcacc
     1801 accagcctgc agtatgctaa cggtcagatc acgttaaacg ggcagaaaat gccactggaa
     1861 gattttgttg ggatgtttgc gatgccggca ttaaacgttc cggctgtacc cgctattccg
     1921 cagcagtaat tcacagccaa aacgccggag gtttttctct ccggcgttat ttcttacttc
     1981 agcataaagt catactgaat actcattttc acatcccagt aattcaggcg atcgcccagc
     2041 gcactgccat cgcgcttttc ccgcccttta tcagcagcaa gaaagaaatt catcccttgt
     2101 aacggccctt cgctgacccg atagttcata ccacctaaga tacccgtgcc ttcaatccgc
     2161 acatctcgtt tactggattc atagccgcga gtcacaatcg gtgcaaatgt cagtgtaaat
     2221 tgcggcgtta aacgatagtt gacgcccaat tgccaggact gcattccttc acggttacga
     2281 tcaagactta atgaaaaggg atagccaatg tcggtatcca ccacggcacc attcattgca
     2341 tggcgcaaat aaccaccact gccaaatacg cttccccacg gcgcattcca ggtcaattgt
     2401 ccactaacca acgccgtttc attgggctgg ctggtattac gacttaaacc ttcgagctgc
     2461 gcgtaaaagc cgagcaactc ccctttcaac gtataattcg ggtcgagcgg tatgcttttc
     2521 tcgagaatgc tttgtatctg attggaaaca tctttctgta ccgatgcccc cagagaatag
     2581 cgactggtat catcatgcca actggcggct aaaaaagaac gtgcgcgatg tttatcgtat
     2641 tttcccgtct ggctgttata caccccttca tcacgaatac cggttgttga atcggtccat
     2701 aaaaatactc gtgaacgcca cattccccac agatcaatat ttggcgttaa cgcccaataa
     2761 cctaatgctc cttcagaaac tgtcggtaat gtacgttgcg aactgccagc cattaacgga
     2821 atacgataag gtaagctacc gtaactataa tccgtcccaa agcgacctaa tttaagcagg
     2881 ctattttcac tgggttttat ttttactgcc cccagggcaa agccaaaact tttatcacca
     2941 tcaagatacc accgggtact catatcagca cgagcaccta atttataaac ataatacgct
     3001 ccgccttcaa cgccaataat attggaataa taaccactat tgaaatcgag caatcctccc
     3061 tgtacccatg cgtaaatatc tcttcccgcg ccagcactgg gcttatccgc cctgcgcaat
     3121 tcgtttttca gtctgatgcg tagtcccgct tcatcgtcag ccagttgcgc cgcgtaagca
     3181 gaggtaaggc cagaggcagc cgtcaggcaa ataacggcca tggccactat ttttctcata
     3241 gttgcacctt tg
//
LOCUS       DAJOWB010000260         3214 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJOWB010000260 DAJOWB010000000
VERSION     DAJOWB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.261"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..699
                     /locus_tag="OHO85_004853"
     CDS             <1..699
                     /locus_tag="OHO85_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2674074.1"
                     /translation="SIFSMSKEDLETRTNELGQFFTIRTSNSSSVPMLLQQRLDEIHF
                     KGTERPINQTIYREFNGISVLWDPIYAPVDLERHPYGTVLIPESTLETTGGTFGEMFL
                     TSRGMISIPINDLARTMGITGTIDQSAITEEILRKFNQFVKPLLPLHIVFDGLTLYLS
                     VVVNEQADMITLNEISDTEKAFCWFETSDTTSLTGVTSISAPITATPGGTIVKATPTF
                     DRTRADDLLLDSDA"
     gene            778..1212
                     /locus_tag="OHO85_004854"
     CDS             778..1212
                     /locus_tag="OHO85_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2674075.1"
                     /translation="MSDVSTNLYKSQLLDYYYQRRAESSINKGSRFLISKAVFGTSSL
                     VTKKGDGTYEIGELPKAFDLAELTSQFCTINLVPTYSGGIITVRMDLDQSQLQEGKNY
                     PFNTLVVLDNENKPIAIICVQEDSLYVGKTYTAVMAINTTTA"
     gene            1224..>3214
                     /locus_tag="OHO85_004855"
     CDS             1224..>3214
                     /locus_tag="OHO85_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCQ2674076.1"
                     /translation="MNDVTVVTSVTYPSPESLALVADVQYHEPYLSAALNRKFRGIVD
                     PGFYAGFLPKPGGGMNLLITSVDGDKTAGAASVDIGEFYQVTIQQRKDISLALSAGKK
                     YAIVLKGRYLLGEDTYQVNTASHIHAAEFVSRTYTDSYQLGDGELLVCTVNIPAGVSA
                     ITQEMIDTSKRINRTIGIDISDSVTSTRSDVAASSLAVKKAYDLAKSKYTAQDASTTQ
                     KGLVQLSSATNSDSETMAATPKAVKSVKELADTKAPIESPSLTGTPTAPTAAQGTNST
                     QIANTAFVKAAITALINGAPGTLDTLKEIAAAINNDPNFSTTINNALALKAPLASPAL
                     TGIPTAPTAAQGTNNTQIATTAYVRAAISALVGSSPEALDTLNELAAALGNDPNFATT
                     MTNALAGKQPLDATLTALAALATGANKLPYFTGKDTVAQTDLTSVGRDILAKTSTLAV
                     IQYLGLRELGTSGEKIPLLSTANTWSARQTFNGGITGALTGNADTATKLKTARTIGGV
                     AFDGSANINLPGVNTTGNQNTTGNAATATKLATARNINGVKFDGSGDININTLVSRGR
                     VTALSGSTQGTAGIQMYEAYNNSYPTTYGNVLHMKGASAAGEGELLIGWSGTSGAHAP
                     VFIRSRRDNTDAAWSEWAQVYTSKDSIPGVNTTGNQNTTG"
BASE COUNT          898 a          776 c          783 g          757 t
ORIGIN      
        1 tctatttttt ccatgagcaa ggaggatctg gagaccagga caaatgaact tggccagttc
       61 tttactatca gaacgtcgaa ctcatcttcc gttccgatgt tgttacaaca gcgtcttgat
      121 gagattcact ttaaggggac tgaacgccct ataaaccaga caatttaccg cgaatttaac
      181 ggtatttctg ttttatggga tccgatatat gcaccggtgg accttgagcg tcatccctat
      241 ggcacggttc taataccaga aagcacactg gagactaccg gcggcacatt cggcgagatg
      301 tttctgactt ccagagggat gatcagtatt cccataaacg acctggcccg gacaatgggg
      361 attactggca cgatagatca gtccgcaatt acagaagaaa ttctcagaaa gtttaatcag
      421 ttcgtaaagc ctctactgcc actgcatata gtgtttgatg ggcttacgct ctatttgtcg
      481 gttgttgtaa atgaacaggc cgacatgatc actttgaacg agatttctga taccgaaaaa
      541 gcattctgct ggtttgaaac ttcggataca acttcgctta ctggagttac gtcgattagc
      601 gccccgatca ccgcaacgcc tggtggcact attgtgaaag cgacacctac gtttgatcgc
      661 acacgcgcag atgatttgtt gctggatagc gacgcctgac aatcaccccg tccgcagggc
      721 ggggtgacaa gttacttctc ttacaatgag gcttcacaac attgattagg gaaaatcatg
      781 tctgacgtct caacaaacct ctataagagt cagttgttgg actattacta tcagcggcgc
      841 gctgaatcgt ccattaacaa aggctctcga tttttaatca gcaaggccgt tttcggtacc
      901 agttcactgg ttactaagaa aggagatggc acttatgaga ttggagaact gccaaaggct
      961 ttcgatctgg cagaactgac cagtcaattt tgcaccatca acctcgtccc aacctactca
     1021 ggcgggataa taactgtccg aatggacctt gatcaaagcc agttgcagga agggaaaaac
     1081 tacccattca acactctggt tgttctggat aacgagaaca agccaatcgc cattatttgt
     1141 gtccaggaag actcgctgta tgtgggcaaa acatataccg cagttatggc cataaacacg
     1201 actacagcat aaggatatgc ttgatgaatg acgttacagt tgtcacatcg gttacttacc
     1261 catcacccga gtcgttggct ctggtggctg atgtgcaata ccacgaacca tatctgtcag
     1321 ccgcgctaaa ccgaaaattc agggggattg ttgacccggg attttatgcc ggtttcttac
     1381 ctaagcctgg cggtgggatg aacctgttaa tcacctcagt ggatggtgat aaaaccgcag
     1441 gcgcggcgtc ggtggatatt ggtgaattct accaggtaac tattcagcaa cgtaaggata
     1501 tttctcttgc acttagtgca ggcaagaaat atgcaattgt gctgaaggga agatacctcc
     1561 ttggagaaga tacctatcag gtgaataccg cgtcacatat tcatgcggct gaatttgttt
     1621 ccagaaccta taccgattca tatcagttag gagatgggga gctgcttgtt tgtacggtga
     1681 atatccctgc tggtgtatct gccattaccc aggagatgat tgatacatcc aagcgtatca
     1741 accgcacgat cggcattgat atttcagact ctgtaaccag taccagaagt gatgttgctg
     1801 cgagttcgct ggcagttaaa aaagcctacg atctggctaa aagcaagtat acggcgcagg
     1861 atgcaagcac aacacaaaag ggattagttc agctcagtag cgcaactaac agcgacagcg
     1921 aaacaatggc ggctacccct aaagccgtta agtctgtaaa agagctggct gataccaaag
     1981 cgccaataga aagcccgagt ctgacaggaa cgccaaccgc gccgacggca gcgcaaggta
     2041 caaacagcac gcagatcgca aatacagcct ttgttaaggc agctataacg gcacttatca
     2101 acggtgcacc tggcacactg gatacgctta aagaaatagc tgctgcgatc aataacgacc
     2161 cgaatttcag cacaactatc aacaatgctc tggctcttaa agctccttta gcaagtcctg
     2221 cattaacggg aatacctact gcgcctaccg ctgcacaggg tacgaataac acgcagattg
     2281 ctacgaccgc ttatgtaaga gctgctatct ctgcattggt cggctcatca cctgaagctc
     2341 ttgatactct gaatgagctt gcagcagcac tgggcaatga cccgaacttt gcgacaacaa
     2401 tgacaaatgc gctggcaggc aaacagcctc tggatgcaac tttaaccgcg ctcgctgccc
     2461 ttgcgactgg tgcaaacaaa ctgccttatt tcactggtaa ggatacggta gcgcagactg
     2521 atttaacgtc agtcggtcgc gatattctgg ctaaaacaag cacactggcc gttatccaat
     2581 accttggttt aagagaactc ggtaccagcg gtgaaaagat ccccctgttg agcacggcta
     2641 acacatggag tgcgcgccag actttcaacg gcgggatcac cggggcgctg acagggaacg
     2701 ccgacaccgc gacgaaattg aaaacagcac gcacgattgg cggtgtggca tttgatggct
     2761 cggccaatat caatcttcca ggtgtaaata caacaggtaa ccagaacacc acggggaatg
     2821 ctgctaccgc gacaaaactt gcgacggcaa gaaacatcaa cggtgttaag tttgatggct
     2881 caggcgatat caacattaat acattggtat ctcgtggccg agttacggcg ttaagcggct
     2941 ctactcaagg cactgctggc attcaaatgt acgaggcgta caacaatagc tacccgacca
     3001 cgtatggcaa cgtattgcac atgaaaggtg cgagtgctgc tggtgagggc gagttgctta
     3061 ttggctggag tggtacgagc ggtgcacatg cgccagtttt cattcgctcc cgaagagata
     3121 acacagatgc ggcatggtct gagtgggcac aggtctatac atcgaaagac tccataccgg
     3181 gcgtaaatac aacgggcaac cagaacacaa cagg
//
LOCUS       DAJOWB010000261         3150 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJOWB010000261 DAJOWB010000000
VERSION     DAJOWB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.262"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(60..587)
                     /gene="mobB"
                     /locus_tag="OHO85_004856"
     CDS             complement(60..587)
                     /gene="mobB"
                     /locus_tag="OHO85_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCQ2674077.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(569..1153)
                     /gene="mobA"
                     /locus_tag="OHO85_004857"
     CDS             complement(569..1153)
                     /gene="mobA"
                     /locus_tag="OHO85_004857"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCQ2674078.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMVF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1223..1492
                     /locus_tag="OHO85_004858"
     CDS             1223..1492
                     /locus_tag="OHO85_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCQ2674079.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1569..2555
                     /gene="srkA"
                     /locus_tag="OHO85_004859"
     CDS             1569..2555
                     /gene="srkA"
                     /locus_tag="OHO85_004859"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCQ2674080.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLMPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2572..>3150
                     /gene="dsbA"
                     /locus_tag="OHO85_004860"
     CDS             2572..>3150
                     /gene="dsbA"
                     /locus_tag="OHO85_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCQ2674081.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVF"
BASE COUNT          793 a          766 c          771 g          820 t
ORIGIN      
        1 ttggcgcaga aaaaaatgcc ggatgcgacg ctggcgcgtc ttatccagcc tactcttgct
       61 tatccgttct gcttttgcat ccactccacc acaaaatcag ccagcccctc aacatcatta
      121 atatccagta atgcgacatc aagattaagc ggcacatcac tggctacagc aataacatgc
      181 ctgtctatca ctaattcttc aggtcgatgt ccggctccat cgcgaaacag cacaatcttt
      241 gcgatctctt catgcttaaa cccttcgacc agaatcaaat ccagctttga ggtatccatt
      301 cgacttgcga gaaactgtag atccagctct tcttcgtctg gtgtttccgt catcaaggcc
      361 catcgttgct ggctggcaac gatggtttgt gccgcgccag ccttgcgcag ctcatagcta
      421 tctttgcctg gcttatcaac atccatatca tggtgcgtat gcttaatcag ccctggacgg
      481 atccctctgg cgcataatgc cgggatcagt tttttcaaaa gcgtagtttt tccggtacca
      541 ctccacgcgg caaaggcgag taacggtatc atcgtttttc ctgccatcgg gcaagctcct
      601 ctggcgtatt cacgttaaca aatgcatctt tatgatcgct gaaatcaacc gcatgaccgc
      661 cagccagacg cataaatacc attacccggc gttctcctgc ttgcagatat tccagtaata
      721 aaggctcaat agcgcggttt accagagcaa tagtcgggtg atcgcgttca ccgtcatgga
      781 cccacacgac aggcgcatct ttgcgctgat gattaagcct ggctgctaaa tcagggggaa
      841 tgtaaggcgt atcgcacggg caaaacaaaa accactcacc agcttcctgc tgcattactg
      901 aaagcattcc tgccagaggg cctgggtaat ccgccagtga atcttcaatc actttcagac
      961 cgcttgcctg atagatttcc tgatgacgat tagcattaac cacgacgtga gagagctgcg
     1021 tcataagcgc gtcagcgaca tgttgccata atggtttgcc gtttaattca agcaatcctt
     1081 tatctacgcc gcccattcgt ctggctttac cgcctgccag cacaacgcct gttatcgtcg
     1141 tcatcagatt caccgatatc gcctctttta ttgtgggatt gaccctgcta acgtgtctgt
     1201 ctcaagagta aggagcatca ctatgaaatg taaacgtctg aatgaagtta ttgaactcct
     1261 ccagccagcc tggcaaaaag agccagacct taacctgctg caatttttgc agaaactggc
     1321 gaaagagtca ggttttgacg gcgaactggc ggatttgacg gatgacattc tgatctatca
     1381 cctgaaaatg cgtgattccg cgaaagatgc ggtgatcccg ggtttgcaga aagattatga
     1441 agaagacttc aaaacggcgc tgttacgcgc tcgcggcgta attaaagagt aaaagcttgt
     1501 aagcggcgcc accaaaatca tcgtgaaatg atatccttcg tcattcgtaa tgttttccgg
     1561 atgatgggat gaataacagc gcttttactt tccagacact acacccggat accatcatgg
     1621 acgctctgtt tgagcatggg atccgggtgg attccggtct taccccgctt aacagctatg
     1681 aaaaccgtgt ctatcaattt caggacgaag atcgtcgacg ttttgtcgtc aaattttatc
     1741 gccctgaacg ttggacagcc gatcaaatcc tcgaagaaca tcaatttgcg ttgcagctgg
     1801 taaatgatga agttccggtc gcagcacctg tggcctttaa cggtcagact ttattgaatc
     1861 atcagggatt ttatttcgct gtttttccaa gcgtcggtgg tcgccagttc gaagctgata
     1921 atatcgatca gatggaagcg gttgggcgtt atttagggcg tatgcaccag acggggcgca
     1981 aacagctttt tatccatcgc ccgaccatcg gtctgaatga atatctcatt gagccacgca
     2041 agctgtttga ggacgctaca ctgatgcctt ccgggttgaa agcggcattc ctgaaagcga
     2101 cagatgagct gatcgccgcc gttacagcac actggcggga agacttcacc gttctgcggc
     2161 tacatggaga ctgccacgcc gggaatattc tctggcgcga tggtccaatg tttgttgatc
     2221 tggatgatgc acgtaatggt ccagctattc aggatttgtg gatgttgctc aatggcgata
     2281 aagccgagca gcggatgcaa ctggaaacta ttattgaagc ttatgaagaa tttagcgagt
     2341 tcgacaccgc tgaaatcgga ctgattgaac ctttacgcgc catgcgtttg gtttattatc
     2401 ttgcctggct aatgcggcgt tgggctgatc ccgcgttccc gaaaaatttc ccgtggttaa
     2461 ccggggaaga ttactggctg cgacaaacgg cgacttttat agaacaggca aaagttctac
     2521 aagaaccccc tttacaatta acacctatgt attaatcgga gagagtagat catgaaaaag
     2581 atttggctgg cgctggctgg tttagtttta gcgtttagcg catcggcggc gcagtatgaa
     2641 gatggtaaac agtacactac cctggaaaaa ccggtagctg gcgcgccgca agtgctggag
     2701 tttttctctt tcttctgccc gcactgctat cagtttgaag aagttctgca tatttctgat
     2761 aatgtgaaga aaaaactgcc ggaaggcgtg aagatgacta aataccacgt caacttcatg
     2821 ggtggtgacc tgggcaaaga tctgactcag gcatgggctg tggcgatggc gctgggcgtg
     2881 gaagacaaag tgactgttcc gctgtttgaa ggcgtacaga aaacccagac cattcgttca
     2941 gcatctgata tccgcgatgt atttatcaac gcaggtatta aaggtgaaga gtacgacgcg
     3001 gcgtggaaca gcttcgtggt gaaatctctg gtcgctcagc aggaaaaagc tgcagctgac
     3061 gtgcaattgc gtggtgttcc ggcgatgttt gttaacggta aatatcagct gaatccgcag
     3121 ggcatggata ccagcaatat ggatgttttt
//
LOCUS       DAJOWB010000262         3077 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJOWB010000262 DAJOWB010000000
VERSION     DAJOWB010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.263"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..542)
                     /locus_tag="OHO85_004861"
     CDS             complement(<1..542)
                     /locus_tag="OHO85_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674082.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIKIQ"
     gene            complement(539..2059)
                     /locus_tag="OHO85_004862"
                     /pseudo
     CDS             complement(539..2059)
                     /locus_tag="OHO85_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            complement(2090..2416)
                     /locus_tag="OHO85_004863"
     CDS             complement(2090..2416)
                     /locus_tag="OHO85_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674083.1"
                     /translation="MKYIIFLFRAIWLALSLLILFFSMHRLSLLDSTRDVSELISLMS
                     YGMMVICFPTGIVFFIALIFIGTVSDIIGVRIDSKYIMAIIIWLYFLSGGYIQWFVLS
                     KRIINK"
     gene            complement(2416..>3077)
                     /locus_tag="OHO85_004864"
     CDS             complement(2416..>3077)
                     /locus_tag="OHO85_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HCQ2674084.1"
                     /translation="EENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDP
                     IGLKGGWNFYQYPLNPISNIDPLGLETLKCIKPLHSMGGTGERSGPDIWGNPFYHQYL
                     CVPDGKGDYTCGGQDQRGESKGDGLWGPGKASNDTKEAAGRCDLVETDNSCVENCLKG
                     KFKEVRPRYSVLPDIFTPINLGLFKNCQDWSNDSLETCKMKCSGNNIGRLIRFVFTGV
                     M"
BASE COUNT          747 a          778 c          663 g          889 t
ORIGIN      
        1 tgctgtatct ttattaaaca ttcgtaagac tcatttttgt tcgggagatg tgctgtcatt
       61 cgagcataag ttggtttctc atctttaaaa tattttacca tgattgaatt atcaaggact
      121 gccaacaact gaactggatc actgctcttt ttttgataga tacccgctct tatataactt
      181 ctaaaccgat cgccgagaaa cgccgttttt tctgctgtat ttatcaactc ctgcaacgtt
      241 cctttgatgg catgattttc attaaggcgt agtgacccga cgcgaattaa gagtctcgcc
      301 ccgcttggtg tgaatttatt atttttacat gtacccagtt ctgtttgcca ataatactca
      361 ccgatgtcct cattctgata ccatctccac atggctttat tatttaagac aggaaacgcg
      421 gaaaaaatag tacccttagc gaaaatagta aaacaatcat gatctttagc ttgtatttcg
      481 caatcagcta taacaccaaa actaacaaat atcatcagca aaaagaacaa aactcttttc
      541 attttcttat tcctctcgat gttctctgtt tcattttagc taaaatttca cccaaatcat
      601 ctatccctgc tgttgctggc ccatcgggtt tattaatgtc tgggaataaa tagggttctg
      661 cacaagtata tcctggtggt aattgagttg ttggccaatt tgtctgtggc aggtaatcac
      721 ttgctgtctt tgtatccatc cctggatact gggaatcatc ctgtggcgta caagtcagat
      781 ttggttttac ccacttcctg caatacagtg tgtagtcgta agggcttaat gctctgaaga
      841 atgttttgaa aacattaggc gcactactgg cctggtcaat aggatatggc agtcttaatt
      901 gtttcgctat gtccggcaat ttacttggat cgggcgtagg cggtttgttg ggtctcgctc
      961 catatccgcc attatttaag tgtccatatt ttgatgccag tcccattgaa tctatatact
     1021 gaaccggatt cagcggatac tgataaaaat tccatccccc cttcagccca atcggatcct
     1081 gagtgatata cctcccctgc agcgggtcat aatagcggtg gcggttgtaa tacaggccgg
     1141 actcctcatc atactgctgc cccggcaggc ggataagctg ctgcagctga tgcgggttct
     1201 cttcattcag caggttgccc cattcatcgt attctgcgca ccattctgtt gccccttccg
     1261 tgctgacaag cgccagcggc aggccgcgat ggtcgcagtg gtacaggtgg atttttcgcg
     1321 ccggcgtgta caccgggtcc atctggtttt gcatctgctc caccgtcagg ccgcacgatg
     1381 ccagccagcg gcggctttcc tcactcaccc ggtcagccag gatttcactt tccagccggt
     1441 cgagcatctg caccagcacc ggcgggaaca ccacactgcc accgtcttcg ccgccggact
     1501 gctgaagggt atccgccagg ctgcggcgct gcgttttcgc ctgctcaccg gtggcggttt
     1561 caaccctgat gagtggcgtg aagctccccg gctgataaat cgtctggatg cgggttctgt
     1621 cgttctgtat tgtggtcagg cggtcgccgt cccagccgta ccaggtcact tgcggtttcc
     1681 gtgacagcga catccagccc gtcaggtccc gttcacgtcg ccacacccgt tttgccaccc
     1741 tgcggcccag cgggtcgtaa agatagcggc tttcgaccag cggctctgca tattgtgtcc
     1801 gcgtgtagtg caccagccgg tgctgactgt cgtaatggta ccggtgggtg cgctcatcat
     1861 ccgtgcggat aaccccttcc gggatgaggt cggttttctc cgtcagcctg ccgtgacggt
     1921 cataccggta aagatagtgc gcgtcacggg cgatacggtt atccggccac atgctgaggg
     1981 cgctgtccgg gtgcagctcc gggtcgggca ggcggttacc tgccgggtct gtggcatacg
     2041 ggatatcaat gcccaggtta catataataa taagagcaat taacttaatt tatttgttta
     2101 taatgcgctt acttaataca aaccattgaa tataccctcc tgacagaaaa taaagccata
     2161 ttattatcgc cataatatac ttactatcaa tccttacgcc aataatgtct gatacagtcc
     2221 ctataaatat tagagcaata aagaatacta tgcctgttgg aaaacatatt accatcattc
     2281 cataagacat gagactaatt aactcgctca catcacgagt ggagtctaat agtgaaagtc
     2341 tatgcatgga aaagaatagt attaaaagcg ataaagccag ccatatagct ctgaataaaa
     2401 aaataatata tttcattaca tcactccggt gaatacaaat ctaataagac gtccaatgtt
     2461 atttccggag cacttcatct tacatgtttc taaagaatca ttagaccagt cttggcagtt
     2521 tttaaatagc cctaaattta taggtgtgaa tatatcaggc aatacagaat aacgcggcct
     2581 tacctcttta aacttccctt ttaaacagtt ctccacacaa ctattatcgg tttcaacgag
     2641 gtcacaacgg ccagcagctt cttttgtatc attacttgct ttacctggcc cccatagacc
     2701 atctcctttt gattctcccc gttggtcttg gccaccacaa gtatagtccc ctttaccatc
     2761 tgggacacaa agatattgat gatagaacgg attcccccat atatctggac cgcttctttc
     2821 accagttccg cccattgaat gcagtggctt aatgcatttt agtgtttcta atcctaatgg
     2881 atctatattt gagatcggat tcaacggata ctgataaaaa ttccatcccc ccttcagccc
     2941 aatcggatcc tgagtgatat accgcccctg cagcgggtca taatagcggt ggcggttgta
     3001 atacaggccg gactcctcat catactgctg ccccggcagg cggataagct gctgcagctg
     3061 atgcgggttc tcttcat
//
LOCUS       DAJOWB010000263         3031 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJOWB010000263 DAJOWB010000000
VERSION     DAJOWB010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.264"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..2056)
                     /gene="traD"
                     /locus_tag="OHO85_004865"
     CDS             complement(<1..2056)
                     /gene="traD"
                     /locus_tag="OHO85_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HCQ2674085.1"
                     /translation="MSFNAKNMTQGGQIASMRIRMFSQIANIILYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEITGGRQLTDNPKEVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGREMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PVSPAINDKKSDAGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAVYEAWQ
                     REQ"
     gene            complement(2309..3022)
                     /locus_tag="OHO85_004866"
     CDS             complement(2309..3022)
                     /locus_tag="OHO85_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HCQ2674086.1"
                     /translation="MMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIWLEPASE
                     RTVFLQFKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKADKMDLRE
                     SQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVEDVNYTM
                     ITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNANKVNLKF
                     EEAKPVLEDQLAKSIANIL"
BASE COUNT          730 a          846 c          707 g          748 t
ORIGIN      
        1 tctgctcccg ttgccatgcc tcataaacgg ccatatccac cacttcaccg gattcactga
       61 tcccgggtgg cagttgctgt tccatctctt cttccggttt cattttcagc tcctgctcga
      121 tcccccctgc cggaacattc acacctgcat ctgacttctt atcgttgatg gcaggagaca
      181 ccggctgttg cggctgttca gcctgagtca cgtcttctcc ggaaacaacc tccgggacat
      241 ccggttcgaa gaggctggcc atctcacgac cttctgcttc ccttgcggca agtacggcac
      301 tcagacggtt ctccatttcc gggttgatgt cacgcggaat aaactccgga gcgaccttcg
      361 gtcgtgtctg atatttcagg gagagtttta ctgccggata cggtccgggc agggtgacat
      421 aacaggtcag atccggcaga gactgaatgt cggaataact gaccagcgtc tgacgctcca
      481 tatccttacc ggtcgatacc ccgtcacgta ccggatcagc accgtaggaa tactgctcgc
      541 tggctttcag gtgctctttc tcaccaattt caccggcagc gaactctgca atcttatggc
      601 tgggagaacg gaaaaaggca cgggtgttca tgacgtcaaa cagcgtggca gccgctttct
      661 caccgtagat atcttccagc tgggcatagg actggatacc aaacacataa cagccaccga
      721 acttacgggc ttccggcagg atctccacca ggtccggcag tttgtgtaac gtgggtaact
      781 cgtcacagaa aaaccacaca cgacggttac ggttttctcc cattgccagc agaccacgaa
      841 tagcaatgga cagccacatg gagatcaccg gcttcaggga ggcatgggtg tcggcgttcg
      901 acgaaataaa cagccagccg tttttctgat cttcccggac accacgcatc cagtcacgga
      961 tggtgaaggg ctcaccgtta tgctcaatcc cctgcaggta acggatggct ttcacgtagt
     1021 tggtcagcac agcacggatg gatatggccg ttttctcgat tttctcttcc accaggttgg
     1081 ccgccggtga attacgcagg aaggtacgca gcttttcaat tttgatggaa agcagtgtgt
     1141 ccaccagttt gctgtagctg cggttggggt cattacgcat caggtatgcc gcttccgcaa
     1201 aaatggtacg tcctgaaccc tgccagaacg ggtcctcttt tgtccccatc gggatcagag
     1261 tatttgcggt attatcaaaa tctggctgtg tcaggcactc cttccacaaa tcccaggcag
     1321 cacagcgtgc atccagcgga ttcaggattt tatcgatgga gggatcatag taacttttaa
     1381 caaattcccc cgaacggtca taaatcacca ccatatcacc acgctgacgg gcgtagttgg
     1441 ccagacgacg gataacctcc gacttaccgg ccccgacagt gccgtgcaga cagaagttct
     1501 ggatttcaga atcccggata atcggcagat cgccaatccg gatatcggaa tccttgccat
     1561 cttttttcag catcctggcc acctctttcg gattgtcagt cagctgacga ccacctgtga
     1621 tttcattttc gctctgttgt ttaccctgac gccccagaat ccaggagaca acaaagaagg
     1681 tgatcaggca gataaccagg gccacgacag acgccagaac gaatgccgac cataactgct
     1741 caccgcacca gatcatatat ttatcatgca gtacctgagc cgcgttcatc cggaaggttt
     1801 tgccgtaata ctggatctca tagaccggct gggacctgat taaatcccgc atgccttcca
     1861 gcgtggtgca ccaccagtaa atgcagccat tcacaaacgt ctgccagctt atttttaccc
     1921 ataaaaccag accaactagg atccagaaaa aaataaacag gcagtaaagt atgatattgg
     1981 cgatctgact gaacatgcgg atacgcatgg acgcaatctg accgccctgg gtcatattct
     2041 ttgcgttaaa actcatgatg atattctgaa gaaagaaagt cagtcggact gattattaat
     2101 gatgaaaaga ttaataaccg ttaaattccg gcttcgccgg caacacttca cggtcgcaca
     2161 aagaatttgc acaaccgctc agtgttttca tatttatttc ggaaacgccg gggagaaata
     2221 aaatttattt tatttctccc cggggttgga caacgagcaa agcgaggcgt cagtcaggag
     2281 gccggtcaga ccagcctccg gaagataatc agagaatatt tgcgattgat ttggccagct
     2341 ggtcttcgag aacaggcttc gcctcttcaa atttcaggtt aaccttgttc gcatttgaga
     2401 ccacacgggt ctggtattta tgctggttac ctgtttcagt actggtctga attttcgcac
     2461 ctgatgtgcc ctgacgcagg gcggcaacat tatccgttgt caccgttgcc ttagtacgct
     2521 ctgcaatctg cacatccgtg atcatggtat agttcacatc ttccaccatc gcatctgcag
     2581 ccatacccac cagaccggca gcaaggccca caccgagtgt ggcaccggca gaattggaat
     2641 tatatccggt gataccggca cctaacgctg caccaactgc tgcgccttca taaccacggt
     2701 tcagccatcc ctgagactcc cgcagatcca tcttatcggc cttcagcaca ttcgcctgaa
     2761 tccagtagta ggctttatcc ggagaagtca ccacctgata cccttttgct ttcacagcat
     2821 cagcaatttt gccctgcagc ccactcatgt ctttatcaga cgtgtttttg aactgcagaa
     2881 ataccgtgcg ttcgctggcg ggttcaagcc agatggtctc actcatctga gtcttcacct
     2941 caaggttacg cttcttgatt gctgtgctca tcgcaccaca ccctgaaagg gccagagtgg
     3001 aactgaccag tgcaaccatc atcaattttt t
//
LOCUS       DAJOWB010000264         2993 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJOWB010000264 DAJOWB010000000
VERSION     DAJOWB010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.265"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1216
                     /gene="traU"
                     /locus_tag="OHO85_004867"
     CDS             <1..1216
                     /gene="traU"
                     /locus_tag="OHO85_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraU"
                     /protein_id="HCQ2674087.1"
                     /translation="GDTRQIINQLIELAFKEYGENNPRLYRASTEELVDSALQDSGLY
                     EKHDATWWARSTWFEVRDMLHNAGYIMAAQRAHYQAMPQLPEVSSMLGHTSLRDVFGT
                     VQRDGSNELLLDYIRRALEQGHNDYPMISGYTRFMINPETRVIAVDLNNVAGDKTPAG
                     RLKTGIMYLLAGQIAGGDFTLPQYRDEVLKQLPREYHEIALKRINQLDQEVKTKVYDE
                     LHNARGIDFIWENLDTQEREQRKFAIRTVLSTQYLRDYPESVLKSANTLWLLRYKPED
                     IPVLRDNFNVPEFMLKRFLKMPEGPAPDGSGVPVLGVFRVKSGTLARILKFTVGPLEL
                     WALNSSPKDSALRKTLTNKLGSVRARKILAENFPRGSATSLIEHRAGQHNSDNVIEEL
                     ASELIRKQGYNL"
     gene            1216..1830
                     /gene="traV"
                     /locus_tag="OHO85_004868"
     CDS             1216..1830
                     /gene="traV"
                     /locus_tag="OHO85_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraV"
                     /protein_id="HCQ2674088.1"
                     /translation="MCTIHITPRCRRPARGQVFLKKLEVKGDSFSFTLPSRVFRLHPA
                     PNPRVIYASDARTQGKVTGMRLLLPSSTDELITTAIWQVSVPDEAPFDVQHVIQWILP
                     SPLASLMSDDTWLWPSVPGTPALSAGDWPVMDTDPSTLRRVRRRSFSIIDNRNRKGYI
                     TRRFQCFPLAPLPENRKYDSLPDLVLHPEQETIKCKEKHYSQPL"
     gene            1797..>2993
                     /gene="traW"
                     /locus_tag="OHO85_004869"
     CDS             1797..>2993
                     /gene="traW"
                     /locus_tag="OHO85_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraW"
                     /protein_id="HCQ2674089.1"
                     /translation="MQRKTLLAALIATLSGTACQAHAYSVTVVASRPVEEQVIPRMEA
                     IKDVLGNILSTQTATGTAINQNSEKLASVIAQNGQATRQQMIFSNETQRLEEARKSFT
                     VPDSICSESASGIATESKSASASAASKLSKGGGVSNRSIRDRLASAANSPVREAYDGA
                     AIHASYCTEAEYARFGGTAVCPSVGEIPGGDSQVRSIYHGAGTADTPAALTWDQKQID
                     AATAYMKNTSRPSAGRALGKGEVNTQSGRTYVGLQNEYNGIIDSASNPQLTLIADSTP
                     NESTRKALAETLQSDSAAAYFDQVASPEAKARGYMSTREFEAFEAGRRYANTAYLVDL
                     QEMQGDNLLRELVRITAQMNWQLNDLKEQIRQGNVISGQQLALTARQYYEKQLGSLEK
                     TINQANA"
BASE COUNT          816 a          793 c          755 g          629 t
ORIGIN      
        1 cggagacacc cgacaaatta tcaaccagct tatcgagctg gcttttaaag agtacggtga
       61 aaataatccg cgactttatc gcgcctcaac ggaagaactg gtggattcag cactgcagga
      121 ctcgggactg tatgaaaaac atgatgcaac ctggtgggcc agatcaacct ggttcgaagt
      181 ccgggatatg ctccacaatg ccgggtatat catggcggct cagcgggcac attatcaggc
      241 aatgccccag cttcccgaag tgtcctcgat gctgggccat accagtctca gggatgtttt
      301 cggtactgtt cagcgtgatg gttcaaacga gcttctgctg gactacatcc gccgcgccct
      361 ggagcaggga cataacgact accccatgat atccgggtat acccggttta tgatcaaccc
      421 ggaaacccgg gtcatcgctg tcgatctgaa taatgttgcc ggtgacaaaa cgcctgcagg
      481 caggcttaaa accggcatca tgtacctgct tgcaggtcag atcgccggtg gtgacttcac
      541 cctgccacaa tacagggatg aagtgttaaa gcagcttcca cgggaatacc atgaaatcgc
      601 tcttaaacgt attaaccaac ttgatcagga ggttaagaca aaagtttatg atgaactgca
      661 taacgcccgg ggcattgact tcatctggga aaatctggat acccaggagc gtgagcaacg
      721 caagtttgct atccgcacgg ttctgagtac gcagtatctg agggactacc cggaaagcgt
      781 tcttaagtca gcaaatactc tctggttact gcgctacaaa cccgaagata tccccgtact
      841 gagggataac ttcaacgttc ctgaatttat gctgaaacgc ttcctgaaaa tgccggaagg
      901 cccggcacca gatggtagtg gtgttcctgt tctgggagta ttcagggtaa aaagtggcac
      961 gcttgcgaga atactcaaat ttaccgtcgg tccactggag ctatgggcgc tgaactcatc
     1021 gccaaaagac agtgccctgc gtaaaacact gacaaataag ttaggttcag ttcgggcacg
     1081 taagattctg gccgaaaact tccccagagg ttctgcaacg tcattaattg agcaccgggc
     1141 gggccaacat aacagtgaca atgttattga agaactggcc tcggagctca tccgaaaaca
     1201 gggatacaat ctctgatgtg tacaattcat atcaccccac gctgccggag acctgccaga
     1261 gggcaggtat ttcttaaaaa acttgaggta aaaggtgaca gtttctcatt cactctgccg
     1321 tcacgtgtct tcaggttaca tccggccccc aatcccagag tgatatacgc ctcggatgcc
     1381 agaacacagg gaaaggttac aggcatgcgg ttgttgttgc cgtccagcac agatgagctg
     1441 ataacaacgg caatatggca ggtttctgtc ccggatgaag caccattcga cgttcagcat
     1501 gtcatccagt ggatactccc ttcaccgctg gcttcgctga tgtccgatga tacctggtta
     1561 tggccatccg ttcccggaac gccggcactg agtgccggtg actggcctgt tatggatact
     1621 gacccttcca ccttacggcg cgtcagacgt cgttctttca gcatcatcga taaccggaac
     1681 aggaaaggtt atatcaccag gcgatttcag tgtttcccac tggccccact acctgaaaac
     1741 agaaaatacg acagccttcc tgaccttgtg ctccatcccg agcaggagac aataaaatgc
     1801 aaagaaaaac attactcgca gcccttatag ccaccctgag tggaacggca tgccaggctc
     1861 atgcttattc agtcaccgtc gtcgccagcc ggcctgttga agagcaggtc atccccagaa
     1921 tggaagccat caaagatgtc cttggcaaca tactgagtac ccagacagcc acgggtaccg
     1981 ctatcaatca gaactctgaa aagctggcaa gtgtcattgc ccagaacgga caggcgacac
     2041 gtcagcagat gattttcagt aatgaaactc agcgcctcga ggaggcacgg aaaagcttca
     2101 ccgtgccaga ctccatctgc agtgaatccg catcagggat tgcaacagaa agcaaaagtg
     2161 cctcagcatc cgcagcctca aaactcagta aaggtggcgg tgtgagcaac cgtagcatcc
     2221 gcgaccgtct ggccagcgca gcaaactctc ccgtgcggga agcttatgat ggcgcggcca
     2281 tacacgccag ttactgtaca gaagcagaat atgcacgttt tggaggaacc gccgtttgtc
     2341 cttctgtggg cgaaatacca ggtggagaca gtcaggtacg ctcgatttac cacggcgcag
     2401 gtacagcgga cacacctgca gcactgacat gggatcagaa gcaaatagat gcagcgacgg
     2461 cttacatgaa aaacacttcc cgcccctctg ccggtcgtgc tctgggtaaa ggagaagtga
     2521 atacccagag tgggcgaacc tacgtcggac tccagaacga atacaacggc atcattgatt
     2581 ctgcgtcgaa tccccaactg acgctgattg ctgacagcac gcctaatgaa agcacacgta
     2641 aagcactggc tgaaacactg cagtcagact cagcggccgc ttacttcgac caggtggctt
     2701 caccagaagc aaaagcacgg ggatatatga gtacccgtga gtttgaagct tttgaggctg
     2761 gccggcgcta cgccaacaca gcatacctgg tggatttaca ggaaatgcag ggagataacc
     2821 tgctccgtga actggtaaga atcactgcgc aaatgaactg gcaactgaac gacctgaaag
     2881 aacagatccg ccagggtaat gtcatctctg gacagcaact ggctctgacc gcccgccagt
     2941 attatgaaaa acaactcggt agccttgaga aaacaatcaa tcaggcaaac gca
//
LOCUS       DAJOWB010000265         2989 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJOWB010000265 DAJOWB010000000
VERSION     DAJOWB010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.266"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            126..458
                     /gene="hdeA"
                     /locus_tag="OHO85_004870"
     CDS             126..458
                     /gene="hdeA"
                     /locus_tag="OHO85_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCQ2674090.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            574..900
                     /gene="hdeB"
                     /locus_tag="OHO85_004871"
     CDS             574..900
                     /gene="hdeB"
                     /locus_tag="OHO85_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCQ2674091.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            964..1611
                     /locus_tag="OHO85_004872"
     CDS             964..1611
                     /locus_tag="OHO85_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCQ2674092.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1653..2183)
                     /locus_tag="OHO85_004873"
     CDS             complement(1653..2183)
                     /locus_tag="OHO85_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2674093.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(2339..2905)
                     /gene="slp"
                     /locus_tag="OHO85_004874"
     CDS             complement(2339..2905)
                     /gene="slp"
                     /locus_tag="OHO85_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCQ2674094.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          878 a          590 c          654 g          867 t
ORIGIN      
        1 ttgaaataaa aatatctgat tttgatattt tctatcaaca tgacatatac agaaaaccag
       61 gttataacct cagtgtcgaa attgattcgt gacggctctt tcactttata gttgaggata
      121 ttacgatgaa aaaagtatta ggcgttattc ttggtggtct gcttcttctg ccagttgtaa
      181 gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa accggtcaac tcctggacct
      241 gtgaagattt cctggctgtg gacgaatcct tccagccaac tgcagttggt tttgctgaag
      301 cgctgaacaa caaagataaa ccagaagatg cggttttaga tgttcagggt attgcaaccg
      361 taaccccagc tatcgttcag gcttgtactc aggataaaca agccaacttt aaagataaag
      421 ttaaaggcga atgggacaaa attaagaaag atatgtaatt ccgggaatgc gttacatcgt
      481 acttccttgc atattgaaca ggccggaata tcttctttaa aagcagctat tcctcctgtt
      541 catatataat ctctatattg aatgggttac aaaatgaata tttcatctct ccgtaaagcg
      601 tttattttta tgggcgctgt agcggctttg tcactggtga acgcacaatc tgcgctggca
      661 gccaatgaat ccgctaaaga tatgacctgc caggaattta ttgatctgaa tccaaaagca
      721 atgaccccgg ttgcatggtg gatgctgcat gaagaaacag tatataaagg tggcgatacc
      781 gttactttaa atgaaaccga tctcactcaa attcctaaag tgatcgaata ctgtaagaaa
      841 aacccgcaga aaaatttgta taccttcaaa aatcaagcat ctaatgactt gccgaattaa
      901 tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt gctgctcctt tttgcatttt
      961 tatatgacag cagaatttat tatacgtctt atactcgcgg caattgcctg tggcgctatt
     1021 ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac gcacacatgt attaattggc
     1081 atgggaagcg ccctgtttat gattgtttcg aaatatggtt ttgctgacgt gctgtcttta
     1141 gatcacgtcg gactcgaccc cagccgtatt gctgctcagg tggtgacggg cgtcgggttt
     1201 atcggtgcag gtaacattct cgttcgtaac caaaatattg taggtctgac gacggcagcg
     1261 gatatctggg tgaccgccgc cataggtatg gttattggca gcggtatgta cgaactgggt
     1321 atttatggtt cagtgatgac cttgttggtg ctggaagtct tccatcaatt aaccttccgc
     1381 ctgatgaaca aaaattacca tttacagctg acattagtga acggtaatac ggtatcaatg
     1441 ctcgactggt ttaagcagca aaaaataaaa acggatttag tttcgttgca ggaaaatgaa
     1501 gatcatgaag tggtcgccat tgatatccag cttcatgcca caacgtcgat agaagacctg
     1561 ttgcgattat taaaaggtat ggcaggggtg aaaggcgttt caattagcta atctgtgtgg
     1621 cagcgtagcc agactcaccg taagcctgaa attcacacca gataatcaat atgctgatgg
     1681 cgtaccagct cattgatccg cttaagattg aggatcatca tgatattgtg tttatggcag
     1741 tagaacgttt ttagcgatat tttcagtttc tccagaatat cttccgttgt catttgattc
     1801 gcaatgcagc tcaaaacctg atgttgcttg cgcgaaagtg acacattcgc ttcaggcaca
     1861 gagcagtaac tgccattgat gagtgcgaca aaatcaatga tagtgatagt ccgcggaaca
     1921 aaggtcaccg gtactttccc cagacagcgt ttaatattaa agggggagaa aataatcaca
     1981 tggacaggtt ttaatcgttc aatttgagtg agaaaattag aatgaaatac attattcatt
     2041 aatgtatcaa taatgacgaa ggcattctga tgcaacatta cgtcgatctc ttcttcgttc
     2101 tgtatatgaa cgacattacc tttactcaga atgtttttca tcgcggtgaa gaacatcgta
     2161 tccctggtaa ttataagaaa cataaccgac tcctggtgcg gacattaact ttataatcag
     2221 tttgccaaag gtaattaaca ggacagggct tataataata atagttagct tactgtatat
     2281 taattttatg taatacatga tgcagatgct gagaaagcat ctgcatcttt cggtggtgtt
     2341 atttgaccag ctcaggtgtt acctgactca ccgcattggt gtagtaaggc gcaccccagc
     2401 ccggttccgg ccagaatgca ccatagccgt aatcccacag gttataggtg gtattaacca
     2461 cttctctcaa atgccacacc tggatgccct gcatattcac ttccaggaag ttatacggga
     2521 ctttattgat aaagcctggt tgttcaccct gaatggtacc gaggatggta acaaagtgat
     2581 tacgatagtt cactggatca aggaagccgc tttgtctggc gagcagtcgg ccctgatagt
     2641 tggcttcaat atcaggcttc gcatagctat ccaacggtaa tacagcgatt tctaacaacg
     2701 tatccgtttt gccattgata acgttgataa ccttcccacc aaagcgcgct tgttgaccaa
     2761 catataaccc cggctggtta tgaacagcaa caaaactttt ttgaatatca ggttggttat
     2821 tgcctttgat attttgcgga attgaactac atgcggcaag caaaaatgaa aggctgagga
     2881 tgagtgcacc ttttgtcatg ttcatgttac tatccttatc aacaaatatt aaagccataa
     2941 tagatatttc tttatagttt tcatctgatt ctgaggtgat atgcctgat
//
LOCUS       DAJOWB010000266         2922 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJOWB010000266 DAJOWB010000000
VERSION     DAJOWB010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.267"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            611..832
                     /locus_tag="OHO85_004875"
     CDS             611..832
                     /locus_tag="OHO85_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase"
                     /protein_id="HCQ2674095.1"
                     /translation="MGYSAAKVSTHIELEKNRGYWRAKGFDRDSCQLSLSRGEEKIER
                     TRGRWRFYDENHKQVKAEPILYTLLKTII"
     gene            840..1871
                     /locus_tag="OHO85_004876"
     CDS             840..1871
                     /locus_tag="OHO85_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCQ2674096.1"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKM
                     LLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRS
                     GLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDLVRSLMENSERCQDIRN
                     LAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVT
                     KLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAK
                     DDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGA
                     MVRLLEDGD"
     gene            1922..2233
                     /locus_tag="OHO85_004877"
     CDS             1922..2233
                     /locus_tag="OHO85_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysogeny establishment protein"
                     /protein_id="HCQ2674097.1"
                     /translation="MRKDFNIDGKYVVLSVSTNIQSPAVIVTVKLNDRMPDIDSISVA
                     FPVKSMRSAEHFVMNATEEEARRGFAKVMSEFGELLGKVNNVLSISSARSKALTASMM
                     K"
     gene            2481..>2922
                     /locus_tag="OHO85_004878"
     CDS             2481..>2922
                     /locus_tag="OHO85_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCQ2674098.1"
                     /translation="MKTIEQKIEQRREWQKAARERAIARQREKLADPAWRESQYQKMR
                     DTIDRRIAKQKERPPASKTRKSAVKIKSRGLKGRTPTAEERRIANALGALPCIACYMH
                     GVISEEVSLHHISGRTAPDCHKKQLPLCRWHHQHAAPAEVREKYP"
BASE COUNT          826 a          646 c          711 g          739 t
ORIGIN      
        1 agccataatt atgcctcact gtattgacaa cacagagcct gaagctctga cctactgttt
       61 cacccatgat ccatgctggg gtaatctaac aacattgcgc tgtgtgtaag atgagcaatg
      121 catagctgta atgccgttgt ataaggtttc cctgtttgct catttccttc tgagccgctc
      181 tacaacgctg aagacacatt aaatagtgaa tccaaagtcg tattacgtaa cgacggcaaa
      241 actataattt attagagcaa ttgtcaaaca actatgaaaa acaatccagt ttttagctgg
      301 tggagtggga tttttctctc tcaatttatt gctctaataa ttattgattt ttgtgcacag
      361 ctggacgtaa actcctcttc ggacctgata acttcgtata gcatacatta tacgaagtta
      421 tcttaagggt tattgaacat gatcaattta cctgtaaatc catacagttc aataccttat
      481 caggtcaaat agtgatcact tgatcatttg atcaaggttg cgctacgtaa aatctgcgaa
      541 atgttggcag tgttagtgct ccagatttcg cgtagcgcac ttagcaccac caatcaatca
      601 gaggtgaaaa atgggatatt cagctgctaa agtgtccact catattgagc ttgagaaaaa
      661 ccgtggttac tggcgggcaa aagggtttga tcgtgatagt tgtcaactgt cattatcgcg
      721 cggtgaagag aaaatagaac gcacgcgtgg tcgctggcgt ttctatgacg agaaccataa
      781 acaggtaaag gcagagccga tcctgtacac tttacttaaa accattatct gagtgttaaa
      841 tgtccaattt actgaccgta caccaaaatt tgcctgcatt accggtcgat gcaacgagtg
      901 atgaggttcg caagaacctg atggacatgt tcagggatcg ccaggcgttt tctgagcata
      961 cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc atggtgtaag ttgaataacc
     1021 ggaaatggtt tcccgcagaa cctgaagatg ttcgcgatta tcttctatat cttcaggcgc
     1081 gcggtctggc agtaaaaact atccagcaac atttgggcca gctaaacatg cttcatcgtc
     1141 ggtccgggct gccacgacca agtgacagca atgctgtttc actggtcatg cgacggatcc
     1201 gaaaagaaaa cgttgatgcc ggtgagcgtg caaaacaggc gctggcgttc gaacgcactg
     1261 atttcgacct ggttcgttca ctcatggaaa atagtgagcg ctgccaggat atacgtaatc
     1321 tggcatttct ggggattgct tataacaccc tgttacgtat agccgaaatt gccaggatca
     1381 gggttaaaga tatctcacgt actgacggtg ggagaatgtt aatccatatt ggcagaacga
     1441 aaacgctggt tagcaccgca ggtgtagaga aggcacttag cctgggggta actaaactgg
     1501 tcgagcgatg gatttccgtc tctggtgtag ctgatgatcc gaataactac ctgttttgcc
     1561 gggtcagaaa aaatggtgtt gccgcgccat ctgccaccag ccagctatca actcgcgccc
     1621 tggaagggat ttttgaagca actcatcgat tgatttacgg cgctaaggat gactctggtc
     1681 agagatacct ggcctggtct ggacacagtg cccgtgtcgg agccgcgcga gatatggccc
     1741 gcgctggagt ttcaataccg gagatcatgc aagctggtgg ctggaccaat gtaaatattg
     1801 tcatgaacta tatccgtaac ctggatagtg aaacaggggc aatggtgcgc ctgctggaag
     1861 atggcgatta gccattaacg cgtaaatgat tgctctaatt ctttgatatt tatggtgaca
     1921 tatgaggaag gatttcaaca tcgacggaaa atatgtagtg ctgtctgtaa gcaccaatat
     1981 tcagtcgcca gccgtcattg tcactgtaaa gctgaacgat agaatgcctg atattgactc
     2041 aatatccgtt gcgtttcctg tcaaaagtat gcgtagtgct gaacatttcg tgatgaatgc
     2101 caccgaggaa gaagcacggc gcggttttgc taaagtgatg tctgagtttg gcgaactctt
     2161 gggtaaggtt aacaatgtcc tttcaatcag ttcagcaagg tccaaagcgt taacagcttc
     2221 catgatgaaa taaaaaaaag cctggcaagg agccaggctg cacaaaagag cggggttgta
     2281 ttccgcatcc aatcaatcaa gaaggagtat agcacacagg tactgaagtg aaaaaatgtg
     2341 attcgcgata aacaaaatat ctaccattgc tctaattgat tgttataatt gagccgcagt
     2401 ttttgtcaac tacgaagacg ttgccattac ttcactcctt gacatcattg gcggccatta
     2461 ggccgccttt tttttgccat atgaaaacaa tcgaacaaaa aattgaacag cgccgcgagt
     2521 ggcagaaggc agccagagaa cgagcgatcg ctcggcaacg ggaaaagttg gctgaccccg
     2581 cctggcgaga atcgcaatat cagaaaatgc gggatactat cgaccgccgt atcgctaaac
     2641 agaaagagcg cccaccagcc agcaaaacgc ggaaaagcgc ggtaaaaata aaatctcgtg
     2701 gcttgaaggg gagaacacca acggcggagg aacggcgcat cgccaatgct cttggcgctc
     2761 tcccctgcat tgcctgctac atgcatggag taatatctga agaggtgtct ctgcaccata
     2821 tctccggtcg taccgcgccg gattgtcaca aaaagcaatt gcccctttgc agatggcacc
     2881 accagcatgc agcaccggct gaagtaagag aaaaataccc tt
//
LOCUS       DAJOWB010000267         2892 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJOWB010000267 DAJOWB010000000
VERSION     DAJOWB010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.268"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..203
                     /locus_tag="OHO85_004879"
     CDS             <1..203
                     /locus_tag="OHO85_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4111 domain-containing protein"
                     /protein_id="HCQ2674099.1"
                     /translation="VTGKIAPKDVAADWAMERLPAQYQPVILEARQAYLGQEEDRLAS
                     RADQLEEFVHYVKGEITKVVGK"
     gene            359..1258
                     /gene="aac(3)-VIa"
                     /locus_tag="OHO85_004880"
     CDS             359..1258
                     /gene="aac(3)-VIa"
                     /locus_tag="OHO85_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-VIa"
                     /protein_id="HCQ2674100.1"
                     /translation="MTDPRKNGDLHEPATAPATPWSKSELVRQLRDLGVRSGDMVMPH
                     VSLRAVGPLADGPQTLVDALIEAVGPTGNILAFVSWRDSPYEQTLGHDAPPAAIAQSW
                     PAFDPDHAPAYPGFGAINEFIRTYPGCRRSAHPDASMAAIGPDAAWLVAPHEMGAAYG
                     PRSPIARFLAHAGKILSIGAGPDAVTALHYAEAVARIEGKRRVTYSMPLLREGKRVWV
                     TTSDWDSNGILDEYAAPDGPDAVERIARDYLARTRVAQGPVGGAQSRLIDAADIVSFG
                     IEWLEARHAAPAAAALKPKQRRD"
     gene            1465..1785
                     /gene="groES"
                     /locus_tag="OHO85_004881"
     CDS             1465..1785
                     /gene="groES"
                     /locus_tag="OHO85_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCQ2674101.1"
                     /translation="MGSPTRKICAAINIKPLYDRVVIKRTEEEKVSAGGIVIPDSATE
                     KPIKGQVVAVGEGKVFDNGNVRAPKVKVGDQVLFGKYAGTEVKLDGTEYLVVKEDDIF
                     AVLG"
     gene            1841..>2892
                     /gene="groL"
                     /locus_tag="OHO85_004882"
     CDS             1841..>2892
                     /gene="groL"
                     /locus_tag="OHO85_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313151.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCQ2674102.1"
                     /translation="MAAKDIRFGEDARARMVRGVNVLANAVKATLGPKGRNVVLEKSF
                     GAPTITKDGVSVAKEIELADKFENMGAQMVKEVASKTSDNAGDGTTTATVLAQALIRE
                     GMKAVAAGMNPMDLKRGIDKAVTSAVEELKKISKPCSTSKEIAQVGSISANSDTDIGE
                     LIAKAMDKVGKEGVITVEEGSGLENELDVVEGMQFDRGYLSPYFINNPQSMQAELEDP
                     FILLHDKKISNVRDLLPILEGVAKAGKPLLIVAEDVEGEALATLVVNTIRGIVKVCAV
                     KAPGFGDRRKAMLEDMAILTGGTVISEEVGLSLEKATINDLGRAKKVQVSKENTTIID
                     GAGDTADIEARIKQIKA"
BASE COUNT          596 a          937 c          898 g          461 t
ORIGIN      
        1 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
       61 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
      121 atcgcttggc ctcccgcgca gatcagttgg aagaatttgt tcactacgtg aaaggcgaga
      181 tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgc cgcttcgcgg
      241 cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca
      301 ggtcaagtct gcttttatta tttttaaaag ccctacacaa attgggagat atatcatgat
      361 gactgatccc cgcaaaaacg gcgatttgca cgaacccgcg acggcacccg cgacgccctg
      421 gtccaaaagc gagctggtcc ggcaattgcg cgacctcggc gtgcgctcag gcgatatggt
      481 gatgccgcat gtgtcgttgc gcgccgtcgg gccgctggcg gacggaccgc agacacttgt
      541 cgatgcgctg atcgaggccg tcggccccac cgggaatatt ctcgccttcg tctcgtggcg
      601 cgattcgccc tatgaacaga cgctgggtca tgatgcgccg cccgccgcca tcgcccaaag
      661 ctggcctgcg ttcgaccccg accatgcgcc cgcctacccc ggctttggcg cgatcaacga
      721 atttatccga acctatccgg ggtgtcggcg cagcgcccat cccgacgcat cgatggcggc
      781 gatcgggccc gatgcggcgt ggctggtggc gccgcacgag atgggcgccg cttatggccc
      841 ccgctcgccg atcgcgcgtt ttctcgccca cgcaggaaaa atcctgtcga tcggcgccgg
      901 gcccgatgca gtcaccgcgc tccattatgc cgaagcggtg gcgcggatcg agggcaagcg
      961 ccgcgtcact tattcgatgc ccttactgcg cgaaggcaag cgcgtctggg tcaccacgtc
     1021 cgactgggat tcgaacggca tcctcgacga atatgccgcg cccgacggcc ccgacgcggt
     1081 cgaacggatc gcccgcgact atctcgcccg caccagggtt gcgcaaggcc cggtcggcgg
     1141 cgcgcaatcc cggctgatcg acgcggccga tatcgtttcc ttcggcatcg aatggctcga
     1201 ggcgcgccac gccgcgccag cggcggcagc gctgaagccg aaacaacgcc gcgactgagt
     1261 cgtcgattat tccggacata tattcctccc tctcgcccag ccgcatagca tcaggcccac
     1321 aagcccgccg cctcttcaac aaggatgtcg ggacgttctt cagtaaagaa cagcttggca
     1381 ccctcgacaa ggatgattgc ccgaggcgat caggcggcga atttttgcgc gcgcgcacgg
     1441 tcgcggcggc gcgcgataaa ggcgatgggt agccccacga ggaagatatg cgcggcgatt
     1501 aacatcaagc cgctgtacga ccgcgtcgtc atcaagcgca ccgaggaaga gaaggtctcc
     1561 gccggtggta tcgtgatccc ggattcggcc accgagaagc cgatcaaggg ccaggtcgtc
     1621 gccgtgggcg agggcaaggt gttcgacaac ggcaacgtgc gcgcgccgaa ggtcaaggtc
     1681 ggcgaccagg tgctgttcgg caagtacgcc ggcaccgagg tcaagctcga cggcaccgag
     1741 tacctggtgg tgaaggaaga cgacatcttc gccgtgctgg gctgagcccg ccgcttcgcc
     1801 gcatcccaac ccgcaatcac agaactgagg taattccgca atggctgcca aggatattcg
     1861 tttcggtgaa gacgcccgcg cccgcatggt ccgcggcgtg aacgtgctcg ccaacgccgt
     1921 caaggcgacc ctgggcccga agggccgcaa cgtcgtgctc gagaagagct tcggcgcccc
     1981 gacgatcacc aaggacggcg tgtccgtcgc caaggagatc gaactggccg acaagttcga
     2041 gaacatgggc gcgcagatgg tcaaggaagt cgcttccaag acctccgaca acgccggcga
     2101 cggcaccacc accgccaccg tgctggccca ggccctgatc cgcgagggca tgaaggccgt
     2161 ggccgccggc atgaacccga tggacctcaa gcgcggtatc gacaaggccg tcacctcggc
     2221 cgtcgaggag ctgaagaaga tctccaagcc ctgctcgacc agcaaggaga tcgcccaggt
     2281 cggttcgatc tcggccaact ccgacaccga catcggcgag ctgatcgcca aggccatgga
     2341 caaggtcggc aaggaaggcg tgatcaccgt cgaggagggc tcgggcctgg agaacgagct
     2401 cgacgtcgtc gagggcatgc agttcgaccg cggctacctg tcgccgtact tcatcaacaa
     2461 cccgcagtcg atgcaggccg agctggagga tccgttcatc ctgctgcacg acaagaagat
     2521 ctcgaacgtc cgcgacctgc tgccgatcct cgagggcgtg gccaaggccg gcaagccgct
     2581 gctgatcgtc gccgaggacg tcgagggcga ggcgctggcc acgctggtgg tcaacaccat
     2641 ccgcggcatc gtgaaggtct gcgcggtcaa ggccccgggc ttcggcgacc gccgcaaggc
     2701 gatgctggag gacatggcca tcctcaccgg tggcaccgtg atctccgagg aagtcggcct
     2761 ctcgctcgag aaggcgacca tcaacgacct cggccgcgcg aagaaggtgc aggtctcgaa
     2821 ggagaacacc accatcatcg acggcgccgg cgacaccgcg gacatcgaag cccgcatcaa
     2881 gcagatcaag gc
//
LOCUS       DAJOWB010000268         2865 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJOWB010000268 DAJOWB010000000
VERSION     DAJOWB010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.269"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            3..1289
                     /gene="tssI"
                     /locus_tag="OHO85_004883"
     CDS             3..1289
                     /gene="tssI"
                     /locus_tag="OHO85_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCQ2674103.1"
                     /translation="MARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQ
                     AVPGRRGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGR
                     VRVKFNWDRYNPSNQESSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPII
                     MGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNT
                     EVLNNRTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKV
                     GSNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGN
                     NVTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDV
                     NGDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            1365..>2865
                     /locus_tag="OHO85_004884"
     CDS             1365..>2865
                     /locus_tag="OHO85_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCQ2674104.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSALWQ
                     VLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPPPPAYRVLTRVVDGFGR
                     TLAFHRAAKGDVAGAVTGVTDGAGRRFHLALTTQAQRAEAFRKQRASSLSSPASPRSV
                     SSSQVFPDTLPAGTGYGTDNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGELRAV
                     YDRSGTQVRGFAYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRFEYG
                     QDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYRLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDE"
BASE COUNT          626 a          749 c          997 g          493 t
ORIGIN      
        1 aagtggcgcg cggaacaagc cgttcgccgg agatatggcc gggacggcga attgtgctga
       61 cggggcatcc gcaggcgaac ctgaaccggg aatggcaggt ggtggcgagc gatcttcacg
      121 gcgaacagcc gcaggcggta ccgggacgca ggggttcagg caccacgctg gataaccact
      181 ttgcggtaat cccggcagac cggacatggc ggccacagcc gttgctgaaa ccgctggtgg
      241 acggcccgca gagcgccgtc gtgacgggac cggcaggcga ggaaatcttc tgtgatgaac
      301 atggccgcgt gcgggtgaaa tttaactggg accgctataa cccgtcaaac caggaaagtt
      361 catgctggat ccgtgtggca caggcgtggg caggcaccgg ctttggtaac ctggcgatac
      421 cgcgtgtggg tcaggaggtc attgtggact tcctcaacgg cgatccggac cagccgatca
      481 ttatggggcg tacctaccac caggaaaacc gcacccccgg cagcctgccg gggacgaaga
      541 cgcagatgac gatccgctcg aaaacctata agggcagcgg gtttaatgaa ctgaagtttg
      601 acgatgcgac agggaaagaa caggtctaca tccacgcgca gaagaacatg aacaccgagg
      661 tgctgaataa ccgcaccact gatgtgataa acaaccatgc cgaaaaaata ggtaacaacc
      721 aggcgatcac cgttaccaat aaccagatcc agaacattgg cgttaatcag atacagacgg
      781 ttggtgtcaa ccaggtggaa acggtgggca gtaaccagat tatcaaagtg ggatcaaacc
      841 aggttgaaaa ggtggggatc attcgtgcgc tgacggtggg tgtggcttac cagacgacgg
      901 taggcggcat tatgaatacc tcggtggcgt tgttgcagtc ctcacaggta gggctgcata
      961 aatcactgat ggtgggaatg ggctacagcg tcaatgtggg gaataacgtc accttctcgg
     1021 tgggcaagac aatgaaggaa aacaccggac aaacagcagt ttattctgcc ggtgagcatc
     1081 ttgaactctg ctgtggtaag gcaaggctgg tgctgacgaa ggacggaagc atatttctca
     1141 atggtacgca cattcatctg gaaggggagt cggatgtgaa cggtgatgcg ccagtgatta
     1201 actggaactg tggtgccaca caacctgtac cggatgcgcc tgtgccgaaa gatttacccc
     1261 ccggaatgcc ggatatgcgg caattttgag tcaggtgcaa aaaattaaaa cggaactggt
     1321 gcagtgacca gataaaccat attaaaagca gaaggaattg attcatgggc ggaaaaccgg
     1381 cggcgcggca gggtgatatg acccgcaagg gactggatat tgtgcagggt tcagcggggg
     1441 tgctgatagg tgcgccgacg ggagtggcct gttcggtatg tccgggaggg attacctatg
     1501 ctaacccggt gaacccgctg ctgggtgcga aggtgctgcc gggcgagacg gaccttgcgc
     1561 tgcccggccc gctgccgttt attctttccc gcgcctacag cagctaccgg accagaacac
     1621 ccgcgccggt gggggtgttt ggtcccggct ggaaagcgcc cttcgatatc cgcttacaga
     1681 tacgcgatga aggcctgata ctcaacgata gcggcggcag gagcattcac tttgagccgc
     1741 tgtttcccgg cgagataagc tacagccgca gcgagtcact ctggctggcg cggggcggag
     1801 tagcggcgca gcacagttcg cagccgctaa gcgcgctctg gcaggtgctg ccggaagatg
     1861 ttcgcctgag tccgcatgtg tacctggcga caaacagcct tcaggggccg tggtggatac
     1921 tcagctggcc ggagcgggtg ccgggggcgg acgaggtgct gccgcctccg ccgcccgcat
     1981 accgggtgct gacgagggtg gtggacggct tcgggcggac gctggccttt caccgggcag
     2041 ctaagggtga cgtggcgggc gcggtgacgg gggtgacgga cggcgcgggc cgccgttttc
     2101 acctggcgct gaccacgcag gcgcagcggg cggaagcctt ccgtaaacag cgcgcctctt
     2161 ccttatcttc cccggccagt ccccgttctg tttcctcttc tcaggttttc cccgacacgc
     2221 tgcccgccgg tacaggatac ggcacggata acggtatccg gctggaggcg gtatggctga
     2281 cacacgaccc ggcatacccg gatgaacagc cgactgcgcc gctggcgcgc tacacgtaca
     2341 cggccggtgg tgaactgcgg gcggtgtatg accgcagcgg aacgcaggtg cgcgggtttg
     2401 cttatgatgc ggagcacgcc gggcggatgg tggcgcacca ttatgcgggg aggccggaga
     2461 gccgctaccg gtatgatgat accggccggg tgacggagca ggtcaacccg gaggggctgg
     2521 actaccgctt tgagtacggg caggaccgtg tgaccatcac ggacagcctg aaccggcggg
     2581 aggtgctgta cacggagggc gagggcggcc tgaagcgggt ggtgaagaag gagcatgccg
     2641 acgggagcat cacccgcagc gagtatgatg aggcggggag gctgaaggcg cagacggatg
     2701 cggcggggcg gcggacggag taccggctgc atatggcgtc gggcgcggtg acagcggtga
     2761 cggggccaga cggcaggacg gtgcggtatg gctataacag ccagcggcag gtgacgtcag
     2821 tgacgtaccc ggacgggctt cgcagcagcc gggagtatga tgaga
//
LOCUS       DAJOWB010000269         2840 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJOWB010000269 DAJOWB010000000
VERSION     DAJOWB010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.270"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..2342
                     /locus_tag="OHO85_004885"
     CDS             <1..2342
                     /locus_tag="OHO85_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HCQ2674105.1"
                     /translation="AGDLTAVIAPDGSRNGIQYDAWGKAVRTTQGGLTRSMEYDAAGR
                     VIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYD
                     EADRLTHRTVKGETAERWRYDERGWLTDISHISEGHRVAVHYRYDEKGRLTGERQTVH
                     HPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWLAGMKLGDTPLVDFT
                     RDRLHRETLRSFGRYELTTAYTPAGQLQRQHLNSLQYDRDYTWNDNGELIRISSPRQT
                     RSYSYSTTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPEQHPDSTLSMWPDNRIARD
                     AHYLYRYDRHGRLTEKTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVES
                     RYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRSRIQTIY
                     QPGSFTPLIRVETATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEI
                     LADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLALISTEGAT
                     AWCAEYDEWGNQLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLRGRYITQDP
                     IGLKGGWNFYQYPLNPVTNTDPLGLEVFPRPFPLPIPWPKSPAQQQADDNAAKALTKW
                     WNDTASQRIFDSLILNNPGLALDITMIASRGNVADTGITDRVNDIINDRFWSDGKKPD
                     RCDVLQELIDCGDISAKDAKSTQKAWNCRHSRQSNDKKR"
     gene            2363..>2840
                     /locus_tag="OHO85_004886"
     CDS             2363..>2840
                     /locus_tag="OHO85_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HCQ2674106.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCEDNVFNILNNMALND
                     KSACVRATAIESTAQRCKKNPIYSPKIVEQSQITAFDKSTNVRRATAFAISVINDKA"
BASE COUNT          767 a          748 c          787 g          538 t
ORIGIN      
        1 tcgccggtga cctgaccgcc gtcattgccc cggacggcag cagaaacggg atacagtacg
       61 atgcgtgggg aaaggccgtc cgtaccacgc agggcgggct gacgcgcagt atggaatacg
      121 atgctgccgg acgggtcatc cgcctgacca gtgaaaacgg cagccacacc accttccgtt
      181 acgatgtact tgaccggctg atacaggaaa ccggctttga cggccgcaca cagcgttatc
      241 accacgacct gaccggcaaa cttatccgca gcgaggatga ggggctggtc acccactggc
      301 actatgacga agcagaccgc ctcacgcacc gcaccgtgaa gggtgaaacc gcagagcggt
      361 ggcggtatga cgaacgtggc tggctgacag acatcagcca tatcagcgaa gggcaccggg
      421 tagcggtgca ttacaggtat gatgagaaag gccgcctgac cggtgagcgc cagacggtgc
      481 atcacccgca gacggaagca ctgctctggc agcatgagac cagacatgcg tacaacgcgc
      541 agggactggc gaaccgctgt ataccggaca gcctgcccgc cgtggaatgg ctgacctacg
      601 gcagcggctg gctggcaggc atgaagctcg gcgacacacc gctggtggat ttcacccgcg
      661 accgcctgca ccgggaaacg ctgcgcagct tcggtcgtta tgaactcacc accgcttaca
      721 cccctgccgg gcagttacag cgtcagcacc tgaacagcct gcagtatgac cgcgattaca
      781 cctggaacga caacggcgaa ctcatccgca tcagcagccc gcgccagacc cggagttaca
      841 gctacagcac caccggcagg ctgaccggcg ttcacaccac cgcagcgaat ctggatatcc
      901 gcatcccgta tgccacggac ccggcgggca accgcctgcc cgacccggag cagcacccgg
      961 acagcaccct cagcatgtgg ccggataacc gtatcgcccg tgacgcgcac tatctttacc
     1021 ggtatgaccg tcacggcagg ctgacagaga aaaccgacct catcccggaa ggggttatcc
     1081 gcacggatga tgagcggact caccggtacc attacgacag tcagcaccgg ctggtgcact
     1141 acacgcggac acaatatgca gagccgctgg tcgaaagtcg ctatctttac gacccgctgg
     1201 gccgcagggt ggcaaaacgg gtgtggcggc gtgaacggga cctgacgggc tggatgtcgc
     1261 tgtcacggaa accgcaagtg acctggtacg gctgggacgg cgaccggctg accacgatac
     1321 agaacgacag gagccgcatc cagacgattt atcagccggg gagcttcacg ccactcatca
     1381 gagttgaaac cgccaccggt gagctggcga aaacgcagcg ccgcagcctg gcggatgcgc
     1441 ttcagcagtc cggcggcgaa gacggtggca gtgtggtgtt cccgccggtg ctggtgcaga
     1501 tgctcgaccg gctggaaagt gaaatcctgg ctgaccgggt gagtgaggaa agccgccgct
     1561 ggctggcatc gtgcggcctg accgtggagc agatgcaaaa ccagatggac ccggtgtaca
     1621 cgccggcgcg aaaaatccac ctgtaccact gcgaccatcg cggcctgccg ctggcgctca
     1681 tcagcacgga aggggcaaca gcgtggtgcg cagaatatga tgaatggggt aaccagctga
     1741 atgaagagaa cccgcaccag ctgcagcagc tcatccgcct gccggggcag cagtatgatg
     1801 aggagtccgg cctgtattat aaccgccacc gctattatga cccgctgcgg gggaggtata
     1861 tcactcagga tccgattggg ctgaaggggg gatggaattt ttatcagtat ccgttgaatc
     1921 cagttacgaa tacagatcct ctggggttag aagtttttcc tagaccattc cccttgccaa
     1981 ttccatggcc caaaagccct gcacagcagc aagcagatga taatgctgca aaagcattga
     2041 caaaatggtg gaacgataca gcatcacaaa gaatatttga ctctctaata ttgaataatc
     2101 cgggactagc attagatata acaatgatag cttctcgtgg aaatgttgca gacacaggga
     2161 taactgatcg tgtcaatgac ataataaatg acagattctg gagtgatggg aaaaaacccg
     2221 acagatgtga cgtacttcag gaactaattg attgtggtga tattagtgct aaagatgcaa
     2281 aaagcacaca gaaagcctgg aattgtcgtc actccagaca gtcaaacgat aaaaaaagat
     2341 agcccttgtg gaggttcctg caatgtcaaa tacataccag aaaagaaagg caagtaaaga
     2401 gtatggttta tataatcaat gtaagaaact aaatgatgat gaattatttc gcttacttga
     2461 tgatcacaat tccttgaaaa ggatttcatc tgccagagta ttacagttaa gaggtgggca
     2521 agacgctgtt agattggcaa ttgagttctg ctctgataaa aattatatcc gtagagatat
     2581 cggagcattt atactcgggc aaataaaaat ttgcaaaaaa tgcgaagata atgtttttaa
     2641 tattttgaac aatatggcat tgaatgataa gagcgcttgc gttcgagcta cggcaatcga
     2701 gtcaacggct cagcgatgca agaaaaaccc aatttattca cctaaaatag tagaacaatc
     2761 tcaaattact gcttttgata aatcgactaa tgtcagacgt gctacagcat ttgctatttc
     2821 tgttatcaat gataaagcaa
//
LOCUS       DAJOWB010000270         2838 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJOWB010000270 DAJOWB010000000
VERSION     DAJOWB010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.271"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..462)
                     /locus_tag="OHO85_004887"
     CDS             complement(<1..462)
                     /locus_tag="OHO85_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCQ2674107.1"
                     /translation="MSDSSQLHKVAQRANRMLNVLTEQVQLQKDELHANEFYQVYAKA
                     ALAKLPLLTRANVDYAVSEMEEKGYVFDKRPAGSSMKYAMSIQNIIDIYEHRGVPKYR
                     DRYSEAYVIFISNLKGGVSKTVSTVSLAHAMRAHPHLLMEDLRILVIDLDPQ"
     gene            complement(631..1440)
                     /locus_tag="OHO85_004888"
     CDS             complement(631..1440)
                     /locus_tag="OHO85_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCQ2674108.1"
                     /translation="MQEEKQHYLYVLVPENGDTFKIGISCGPLARFKGLQVNPDFALS
                     RVYRGTRLAIVNLERALHATFFPWNAPWEKSAGGGHTEWFTRECLDKVLAHIEYLNDM
                     WGGILERIKSNDLLQRPVDAARSFEKELDVTSIVTFKDDAGMRDVAYVSISGYEPDAI
                     RAQCELLKAMFTLRTKYPWETGRVCFPMEELTATIDSQLYHDNPEKFFSLLAGNGLNC
                     VSGLGRSRIQHASLFGPFFYDRNGCFEAELPALTRAIDTIDFERLFAALNK"
     gene            complement(1733..2617)
                     /locus_tag="OHO85_004889"
     CDS             complement(1733..2617)
                     /locus_tag="OHO85_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCQ2674109.1"
                     /translation="MPEESKGFLSVEEVAGNTGEIHSLKPNNNSTIQPIALLRLGVFV
                     PTLKSTNVALRRGSSVTTNTTNATEELSSLKIVEQEGYEGIEIHGPRLDMDTDFKVWV
                     GITSALFDYAPDDDGIITLPFSEFADRCGYPRKRLSKAFRKSIDDSLTRIQQTVVKFR
                     FPAAKGHLNNINVNLLAYSSLNTELDVIEIQPQKQLSELYYVDYKRILKLKMLDKLGR
                     KETAKVLYTFFEALPANPAPVSIERLRARLNLKSSVSVQNSVIRKAMKDLEAIEYLKF
                     SEIKNGRKIGFQIHKRNP"
BASE COUNT          770 a          657 c          621 g          790 t
ORIGIN      
        1 ttgcggatca aggtcaataa ccagaatcct taaatcttcc atcagaagat gagggtgagc
       61 acgcattgca tgcgccagag aaaccgtcga tacagtttta gacacaccgc ctttaagatt
      121 ggagatgaaa atcacatacg cttcgctgta gcgatcccgg tattttggca ctccgcgatg
      181 ttcatatatg tcaatgatgt tctgaattga catcgcatat ttcattgaag agccagcagg
      241 gcgtttatcg aaaacataac ccttttcttc catttcactt acggcatagt caacgttcgc
      301 tcgagtcagt agaggcagtt ttgccagtgc cgctttcgca tagacctggt aaaactcgtt
      361 cgcgtgtagc tcatcctttt gcaactgtac ttgttcagtc agaacattga gcattctgtt
      421 tgctctttga gcaaccttgt gaagctggct ggaatcactc atcgaaagtc atcctttatg
      481 ctgtattttt gaatttaatt aaaaatactg cataaaataa taatgtatgc gtagatgctt
      541 gtacatagca ttctctgcat gtttggttca ttttgcacga tagagagttg caaggagggc
      601 acaaaaaagc cccgttcagg ggcatcagtg ttatttgtta agagctgcga ataatcgttc
      661 gaaatcgata gtatctatag cacgcgtaag cgccggaagt tcagcctcaa agcacccgtt
      721 tcgatcgtaa aagaagggac cgaagagcga ggcatgttgg attctacttc gccccagtcc
      781 ggacacacag ttaagcccat taccggctaa aaggctaaaa aacttctctg gattatcgtg
      841 gtaaagctgg gaatcaatgg tggcggttaa ctcttccata gggaagcaca cccgccctgt
      901 ctcccaggga tatttagtcc gaagcgtaaa catcgctttc agcaattcac attgagcgcg
      961 gatcgcgtca ggttcatagc cagatatgga gacataagcc acatccctca ttcctgcgtc
     1021 atctttgaaa gtcacgatag aagtaacatc cagttctttt tcgaaagagc gagcagcatc
     1081 tactggacgc tgaagtaaat cattcgactt aatccgctcg agaatccctc cccacatatc
     1141 atttagatat tcgatatgag ccaaaacctt atcaagacac tctcgtgtaa accattcagt
     1201 atgcccgcca ccggcgcttt tctcccacgg cgcattccag gggaaaaagg ttgcgtgtaa
     1261 agcccgctca agattaacta ttgccaaacg cgtaccacga tagacccgtg aaagcgcaaa
     1321 atcgggattc acttgtagcc ctttaaaccg tgccaatgga ccacatgaaa tgccgatttt
     1381 aaaagtatct ccgttctccg gcaccagaac gtagaggtag tgttgtttct cttcttgcat
     1441 atcaatacca ctgcttgatg agaaccgcgc aaatgttgac tatgcgcgaa agttaatgtg
     1501 aatagttgac tatgcgcgat gtgactacag tcaaaagttg actgtagtcg atttaactcc
     1561 accaaaaatc gactatgtaa gatattgtcg ggagaaacgt tgactatacg cgatgaaatg
     1621 accctaaaag ccatctcaat agcagcttgc agaatattga cgccagcaaa aatccaccag
     1681 cgtcaacgaa tgtcgcctat agtcaacttc tcgctatcgc atatagtcaa tattatggat
     1741 tgcgcttatg gatctggaag ccgattttcc tgccgttttt tatctctgaa aatttaagat
     1801 attcaatagc ttccaaatct ttcatggctt ttctgataac gctattttgc acgctaacgg
     1861 atgatttgag attaagcctc gctctaaggc gctcaatgct gacaggtgcc gggttggcgg
     1921 gtagagcctc aaagaatgta tacagtacct tggccgtctc tttgcgccct agtttatcca
     1981 gcatcttcag cttcaggatt cgcttatagt caacatagta aagttcagat agctgtttct
     2041 gcggctggat ctcgataaca tcaagctcgg tattcaggct gctatatgcc aacaagttga
     2101 cgttaatgtt attgagatga ccttttgccg ccgggaagcg gaatttgaca actgtctgct
     2161 gaatgcgtgt cagagagtca tcaatacttt tacggaacgc ctttgaaagg cgcttacgtg
     2221 gatagccgca tcgatcggca aactcggaga atggcagggt gattatgccg tcatcatcag
     2281 gtgcgtagtc aaacaacgcg gaggttatgc ccacccacac cttaaaatca gtatccatat
     2341 ccaggcgtgg accatgaatt tcaattccct catagccttc ctgctcaaca attttgaggc
     2401 ttgatagttc ttcagttgcg ttcgttgtgt ttgttgtaac tgacgatccg cgacgtagcg
     2461 ccacattggt agattttaag gttggcacaa acacacctaa gcgcaacaaa gcgatgggtt
     2521 gtatagtgct gttgttattg ggcttcaggc tgtggatttc tcctgtattt cctgcaactt
     2581 cttcaacgct aaggaagcct ttactttctt ccggcatcac ggtttctcca tgtgtggcgc
     2641 ggcctgacgt caatttggat ggctgttatc aacagctgtg aatattcaga ctctaaaatc
     2701 gcttacagtc aatgtttctg tcgcgtatag tcaacaataa atcgcgtgca gtcaacaata
     2761 aatcgtgcac agtcaacata aaatcgcgta tagtcaatgt tgatcccatt tcaggccagt
     2821 aatgacgcgg cttacagc
//
LOCUS       DAJOWB010000271         2826 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJOWB010000271 DAJOWB010000000
VERSION     DAJOWB010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.272"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(538..1668)
                     /locus_tag="OHO85_004890"
     CDS             complement(538..1668)
                     /locus_tag="OHO85_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674110.1"
                     /translation="MTGRKNKCVRKNCNNQVFSVKMAVEKRLCRKHYERFCELKKRRE
                     QNPVHRLNEALPRKIKGPKSKKKLVKEREIINSRKKHKQEQGPSLAELNKKVREILKT
                     PAWRTLETRIRRNGITFKSKAELDVLSELYAIYSWVSYCKRSDNYSVWFLNCPDVNIN
                     LHVCECRSSESDCYLPSRFIILPAPLRQKVKRKYSANSSGVSGHNDSKSLYKTVLSVC
                     GGKRETELFFYSLPVKTNHHPYWIKSGVVKRFPPVFGLLFYEQERLTGGVFRYVLIRF
                     YNTFKENLFFVEIVSCCFLVELFHDIDSFFINQRLSKLESFIDKWREGKEIWIKAYLQ
                     LIHNTLGITETEKLVNLYNSFFTDKVVIWDESVKKVKWIYEK"
     gene            1853..2737
                     /locus_tag="OHO85_004891"
     CDS             1853..2737
                     /locus_tag="OHO85_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCQ2674111.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHDVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQLGERRMTLVDLPGIGETPQH
                     DQEYRTLYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNDTEKCPSRQQELSLATVTARVATLFPSSFPVLSVAAPVGWNLPAFVSLMI
                     HALPPQATSAVYSHIRGENRSEQAQKHAQQTFGDAIGKSFDAAVARFSFPAWMLHLLR
                     KARDRIIHLLVTLWDRLF"
BASE COUNT          805 a          677 c          475 g          869 t
ORIGIN      
        1 tatcataccg acattagcaa cattctcaga cattcatgca gactgaatgt tgattacccg
       61 ttttcacatc aggcatggtt ctttactgac caaaaaacaa actggtttac tcttgaatta
      121 ttttctctac ctcagaaaac atggtctcag gaatctgcta tggtttcata gtttctgttg
      181 ttcttttcat aaaagttata agtcattgtc ataatcatac gataatcact ttatgttagt
      241 ttttactgtc acacgctact gactttttca gtcattgcca gaaaggggct ctttttactt
      301 ccatttcacc actcaacaaa tgcaaaactt gtccagtaaa gcggaccacc tcttgctcac
      361 aagcaacgaa tcatctccag gaacagccct caaacattgc agaaacaaaa acaacaaaac
      421 cctcattatc actgatgttc ggaaaatttc ccgaaacaaa aaaaacgaaa tcaaaatgag
      481 actcattatc atggatgtag tcaaaaagac gttatggtgg ctttcagtta aatggtacta
      541 tttctcataa atccacttaa ctttttttac tgattcatcc cagataacca ctttatcagt
      601 aaaaaatgaa ttatatagat tgacgagttt ttctgtttca gttattccca atgtattatg
      661 tatcagttgt agatatgctt ttatccagat ttctttaccc tccctccact tatctataaa
      721 actttccagc ttacttaatc gttgatttat aaaaaacgaa tcaatatcat gaaaaagctc
      781 aacaagaaaa cagcacgaga caatctcaac aaaaaacaaa ttctccttaa aagtattata
      841 aaaccttatc aacacataac gaaaaacacc tcctgttaat ctttcctgtt cataaaacaa
      901 caaaccaaaa acaggaggaa atcttttaac cacaccactt ttaatccagt aaggatgatg
      961 attagtcttt actggcagtg aataaaagaa caattcggtt tctcttttcc caccacaaac
     1021 agacaaaact gtcttataaa gactttttga atcattatgt cctgataccc cagacgaatt
     1081 agcagagtat tttcttttta ctttttgcct taatggagct ggaagaataa taaaacggga
     1141 tggcagataa caatcgcttt cagaagaccg gcattcgcac acatgaagat ttatatttac
     1201 atccggacaa ttcaaaaacc acacagaata attatcgctc cgtttgcaat atgaaaccca
     1261 tgaataaatt gcataaagtt cagaaagtac atctaactct gcttttgatt taaatgtgat
     1321 accgtttctt cgaattctcg tttccagcgt tctccatgcc ggagttttca gtatctcacg
     1381 aactttttta ttaagttcag ccagcgatgg tccctgctcc tgtttatgtt tcttcctgct
     1441 gttaatgatt tctctttctt ttactaattt tttcttactt ttgggtcctt ttatctttct
     1501 tggcaatgct tcatttaatc tgtgaacagg attttgctct cttctttttt tgagctcaca
     1561 aaacctctca taatgttttc tgcacagtct tttctccact gccattttta ctgagaaaac
     1621 ctgattgtta caattttttc tgacgcattt gtttttacgt ccggtcatcc tctcatctcc
     1681 tgaaaccgca gtatgtcatt agttcccgtt cggaaaaact cacttcatac tccattacac
     1741 tttaacgatc atgtctgtaa ttcagacatt ccatcatttt tcacgtccat attacatgaa
     1801 tgataaaccg gattagtgtg atccggttca gcgaaatcaa caggataacg gtatgtcatt
     1861 cagccattct tccctctccg cacaagtaaa atcatatctg acgtttcttc ctgaagaaat
     1921 tcgccagaaa atccttgaac atctccacga tgttattcat tacgagcccg tgattggcat
     1981 tatgggtaaa tccggcacag gcaaaagcag cctgtgtaat gccatttttc agtcccgtat
     2041 ctgcgccacg catcccctga acggctgcac ccgccaggct catcgtctta ccctgcagct
     2101 cggtgaacgc agaatgacgc tggtcgatct gcccggcatt ggtgaaacac cgcagcatga
     2161 tcaggaatac cggacacttt atcgtcagct gctaccggaa ctggatctga ttatctggat
     2221 cctacgggct gatgaacgtg cgtatgctgc cgatattgcc atgcatcagt ttttactgaa
     2281 tgaaggtgca gatccctcgc gttttctgtt tgttctcagc catgccgatc gcgtgtttcc
     2341 tgctgaagaa tggaacgaca cagaaaaatg cccgtcccgt cagcaggaac tctcactggc
     2401 aacagtcaca gcccgggtgg caaccctgtt cccttcatca tttccggtac tctccgttgc
     2461 cgcacctgtc ggctggaatc ttccggcgtt tgtgtcgctg atgatccacg cactgccacc
     2521 acaggcaacc agcgcggttt attcacatat ccggggggaa aaccgctctg aacaggccca
     2581 gaaacacgca caacaaactt ttggtgacgc catcgggaaa agctttgacg ctgccgttgc
     2641 ccggttcagc tttccggcct ggatgttaca tcttctgcgt aaggcccggg accgcattat
     2701 ccacctgctg gtcacactgt gggatcgtct gttctgacat actcacgccg acaggatgtg
     2761 tcgctggatt aacaagcatt cttcttttta tgaaataatg cttaaaaatc atataattaa
     2821 aagaat
//
LOCUS       DAJOWB010000272         2777 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJOWB010000272 DAJOWB010000000
VERSION     DAJOWB010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.273"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(284..1464)
                     /locus_tag="OHO85_004892"
                     /pseudo
     CDS             complement(284..1464)
                     /locus_tag="OHO85_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            2051..2280
                     /locus_tag="OHO85_004893"
                     /pseudo
     CDS             2051..2280
                     /locus_tag="OHO85_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            2623..2766
                     /locus_tag="OHO85_004894"
     CDS             2623..2766
                     /locus_tag="OHO85_004894"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674112.1"
                     /translation="MSLDPLACGHDNVKQSKIVALTVSLGDKKAQYSRWQPDNLTVDA
                     ETK"
BASE COUNT          691 a          674 c          686 g          726 t
ORIGIN      
        1 gattattttc tttgttaaaa aagacaaacg gtattaactg atgtatttac tatttaccgc
       61 tccctgcagg ggggattatc agaatccgcg gttattcatt atttatatac attcaataaa
      121 aaagtaacct cataaaaaat cctgtactca cagggtgatg cgggataact gacgtaagga
      181 gtgctgcagg gatttgatgg tcccttgtgc tgatatgaat acggatattt tatacagaat
      241 acttttcagt acaagcgact gtattacagt ctgccatata tgattacctg tgatcaggaa
      301 gaataagtgg ctgaggtttc aaactgtctg ccggtatatt cctctctccc ttaaaaacca
      361 taacaggtta gttatcttcg tctgtcagcg gattgaatgg cggtatattt tcagtacaaa
      421 taccggtcat ccactgaaaa atacctgcga aatgacgggc actgattttt ctactgacgg
      481 gctgatgaga cgttatgtca ctggcggtaa taatcagtgg cacactgtag cctccctgca
      541 catgaccata atgatgaaca ggattggcac tgtcgctgac cgacagccca aggccagaaa
      601 agtaaagcat ggcgaaatga cgtgagtacc ggcgaaggat accatcaagc tggccaagaa
      661 agttatccag tttactgatg ctggcgaggt aacaggcaat ttttcgggga tactgctcca
      721 ggtaattatt cggctaggag ttaaggctgt cacacggatt tggatgagaa cccatcatgt
      781 gcaggaaaat tatcttcgga gaggatgtat ccgccagcgc acgttctgtt tcctgtaaca
      841 acagtttgtc atctgtttta cgggaagcga agctgccttt cttgaggaaa gtggcatgct
      901 ccgcatcaga agcaataaca gagatgcgtg tgtcatgctc ccccagtttt ccttgattgg
      961 atatccacca tgtgctgtat cctgcttttg ctgccagcgc caccacattg ttgccggaat
     1021 cagggttctg ctcatagtca taaatcagtg tccggctcag ggaagacact gtactggagg
     1081 ctgtcgaggt ataatcgtca ataaataaac cgggtgccgt attcagccac ggtgtggttg
     1141 gtacgggata gtcatacact gacatataat ccctgcgcac actctcacca gtgacaataa
     1201 caatcgtatc atataacggt gctcccggct ggattttcca gttgtcagcc ccgtgctact
     1261 cagttgttca taacgctgca tttcacgtaa tgtgtcagtt gtccccacaa ctgttccttt
     1321 aaccatccgc aatggccaac ggtttacaga gcataatacg aatagccgta gagccagcca
     1381 gttacggtgg tcgcgatggt gtgtccgcca gaaaatcacc acgaatacca gaatcgcggc
     1441 actgaccaga aaatgataaa caggaaacac ggggcggttc actttgcttt ctcctgtagt
     1501 gttttctgta ggttctgaac cggttctagc ggggttgtga ggctgatggc aatcgccggg
     1561 ctcttcctgc atgttacact ggtactgcaa gagggggcct tcgctcctct gacattactc
     1621 agtattttca ctactacacc ccatctgcca tccataacaa ccgattcacc ccctcgcgag
     1681 gcgttgttgc ccgtcacccg gacagacacc gttatggact tctcttgttg cacgatcgat
     1741 caattgttat gcatgctgac accacaaccc cggtgggtat gaccgatgcc tttctcactc
     1801 tcttcccggt catcgtcggc cttccccgtt attgtggcgt gtcagcaccc tcgacggcat
     1861 ttcgatggct gctcagtgtt cactcacgtt acggcccgca taacccgttg acttcctgaa
     1921 gaagtcgttt cagggagaga ttcagccctt ttgttacctc ctggaccgct tcttgtcctt
     1981 ccagtcggag cgagagttag ccggggcgga ctttcaccga cggaaagatc gtgcatttca
     2041 aggcacacac aacaatatct gcgagcgtgc tatccgtccg gtcgttatgg gacgaaaggc
     2101 ctggttgttc gctggttcac tggtggccgg gaaccgcgcg gcacagataa tgagccttct
     2161 gggaaccgca gtctggagcc acatgcttgg ctgacggacg tcctgacgcg tctgccggag
     2221 tggccggagg agaggttggc tgagttactg cctcttgagg gctttacctt cttcgggtaa
     2281 gtgatacctg ccgtcaggtg tttgtgcacc gggccataac ctgcagttgg gaattgaacg
     2341 cctgacaaca ggaaatgagc tagagcagta aacacgcggg ctgacaatgg gtcagaatat
     2401 gagttagccc ggcgttattc ctggggtcag tacttcagat ctagcctccg gtaccagact
     2461 agctagcccc tacgcaacca aataaatcaa ccatcgtcag agtgcaatcg ttcatataca
     2521 ctgtcggtga tgcgctaaaa aatgcgttaa tgttgagttc ctcgaaccct taccaattaa
     2581 gggacatagc cagatacgca actggctgat gcaagcagtc taatgtcttt agatccgctg
     2641 gcctgcgggc atgacaatgt caaacagtca aagattgtgg cgttgacggt cagtcttggg
     2701 gacaaaaaag ctcagtattc tcgttggcaa ccagataatt taacagttga tgctgaaacg
     2761 aaataggcat cttggtt
//
LOCUS       DAJOWB010000273         2776 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJOWB010000273 DAJOWB010000000
VERSION     DAJOWB010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.274"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            44..2323
                     /gene="ydeP"
                     /locus_tag="OHO85_004895"
     CDS             44..2323
                     /gene="ydeP"
                     /locus_tag="OHO85_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCQ2674113.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKNGAQAVTVEDSMSMIHASRGMLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2571..2768
                     /gene="safA"
                     /locus_tag="OHO85_004896"
     CDS             2571..2768
                     /gene="safA"
                     /locus_tag="OHO85_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCQ2674114.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
BASE COUNT          746 a          643 c          675 g          712 t
ORIGIN      
        1 ctattacaaa tcctaataat tcatttccac acaggataag tagatgaaga aaaaaattga
       61 atcctaccag ggtgctgccg gtggttgggg tgctgttaaa tccgtagcga atgcagtacg
      121 taagcagatg gatatacgcc aggatgttat tgccatgttt gacatgaata agccagaggg
      181 ctttgactgt ccgggttgtg catggccaga tcctaagcac agtgcgtcat tcgacatttg
      241 tgaaaacggc gcaaaagcaa tcgcctggga agtcacggat aagcaggtaa atgcctcttt
      301 ctttgctgag aatacggttc aatcattact tacctgggga gatcacgagc ttgaggctgc
      361 gggacgtctc actcagcctt tgaaatatga tgccgtcagc gactgttaca agccattaag
      421 ctggcaacaa gctttcgacg aaattggcgc acgccttcaa agctatagtg atcccaatca
      481 ggttgaattc tatacttcgg gccgcacttc caatgaagct gcctttcttt atcagctttt
      541 tgcccgtgaa tacgggagca ataactttcc cgactgctcc aacatgtgcc atgaaccgac
      601 aagcgtgggt ttggcagcga gtatcggtgt aggtaaaggg accgtgttgc tggaagactt
      661 tgagaagtgc gatttagtca tttgcattgg gcataaccct ggtacaaacc accctcgcat
      721 gctgacttcg ttgcgcgctt tagtgaaacg gggagcgaaa atgatcgcca tcaatcctct
      781 acaggaacgt ggcctggagc gatttaccgc accgcaaaac ccgtttgaaa tgctgacgaa
      841 ctctgagact cagttggcca gtgcctacta taacgtgcgc attggtggcg atatggcgtt
      901 gctcaagggg atgatgcgcc tgttaattga gcgcgatgat gctgcaagcg ccgcaggtcg
      961 gccttcattg cttgatgacg agtttattca aacgcatacc gtcggctttg acgagctacg
     1021 ccgtgacgtt ctcaattccg agtggaaaga tatcgaacgt atttctggac taagtcagac
     1081 acaaatcgcc gaactggctg acgcatatgc cgctgccgaa cgcaccatta tctgttacgg
     1141 aatggggatc actcagcacg aacatggtac ccagaacgta cagcaactgg tcaatctgct
     1201 gttgatgaaa ggtaacattg gcaagcctgg tgcgggtatc tgcccactac gtggacactc
     1261 taatgtacag ggcgaccgaa ccgtcggtat caccgagaaa ccgtctgcag agtttctggc
     1321 tcgtctgggt gagcgctatg gcttcacccc acctcatgca cctggacatg ctgcaattgc
     1381 cagcatgcaa gcaatatgta cggggcaggc tcgagcattg atctgcatgg ggggcaattt
     1441 tgcgctggca atgccagatc gggaagcgag cgctgtaccg ttaacgcaat tagatttggc
     1501 ggtacacgta gccactaagc ttaaccgctc tcatctgttg accgcacggc atagctatat
     1561 tctgccggtt ctgggacgta gcgagattga catgcaaaaa aacggtgcgc aggcggtaac
     1621 cgttgaggat tcaatgtcga tgattcatgc ctcgcgtggc atgttaaaac ccgccggtgt
     1681 aatgctgaaa tcagagtgtg cagtggtcgc gggaatcgcg caggcagcac taccccagag
     1741 cgtggtagcc tgggagtatc tggtggaaga ttatgatcgc attcgcaatg acattgaagc
     1801 tgtgctgcca gagttcgccg actataacca acgcatccgt catcccggtg gttttcacct
     1861 gataaatgca gctgctgaaa ggcgctggat gacgccgtca ggtaaggcta atttcattac
     1921 cagcaaaggg ctgttagaag atccctcttc agcgtttaac agtaagctgg tcatggcgac
     1981 agtacgcagt cacgatcaat acaacacgac gatttatggt atggatgatc gctatcgagg
     2041 ggtattcggt caacgagatg tggtctttat gagtgctaaa caagctaaaa tttgccgtgt
     2101 aaaaaacggc gaaagagtta atcttattgc gcttacgcca gacggtaagc gcagctcacg
     2161 ccgcatggat agattaaaag tggtcattta ccctatggct gaccgctcac tggtgaccta
     2221 ttttccagaa tcgaatcaca tgctaactct tgataaccac gatccattaa gtggcattcc
     2281 tggttataaa agtattccgg ttgaattaga accatcaaat taattcgcct cctcatttct
     2341 tctgctgtca tccgcacagc agaagaattt ctcattgact attatcgcgc aatttgctca
     2401 cattgattaa attaaaccac acactataag atataaacct ctgcctacag ctgtaagaaa
     2461 ctccgctcag tactgaagtg ccagtcctat ttcctctttt ctctagcctg ttatattaag
     2521 catactgatt aacgattttt aacgttatcc gctaaataaa catatttgaa atgcatgcga
     2581 ctacagtgaa aaacaaaatc acgcaaagag acaactataa agaaatcatg tctgtaattg
     2641 tggttgtctt attactgacc cttacgttga tagccatttt ttcggcaatt gatcagctgg
     2701 gtatttcaga aatgggtcgc attgcaagag atcttacaca tttcattatc aatagtttgc
     2761 aagactgaaa ataaac
//
LOCUS       DAJOWB010000274         2714 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJOWB010000274 DAJOWB010000000
VERSION     DAJOWB010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.275"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            607..927
                     /locus_tag="OHO85_004897"
     CDS             607..927
                     /locus_tag="OHO85_004897"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCQ2674115.1"
                     /translation="MQQFRHWQNRLNTIDPARIKLYSETVRVWNQSRGSAGARALADM
                     LIQNGIPMSSYRARRLMKHLNLRSAQPGKYQYKNARQEHTSLPSLLECRFSVLEAEQV
                     WILK"
     gene            complement(1601..2530)
                     /locus_tag="OHO85_004898"
     CDS             complement(1601..2530)
                     /locus_tag="OHO85_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2674116.1"
                     /translation="MNSIFTEENLLAFTMAARYRSFSKAAKELGLTTSAISYTIKRME
                     MGLDVVLFTRTTRSIELTEPGHYLFCKATDLLNDYHSIIRRVDAIAQGIKSRVRICIN
                     QLLYTPQHTARLLQVLREHFPTCQIAITTEVYNGVWDSLINNQADIAIGAPDTLLDGG
                     GIDYIEIGMIRWLFAISPAHPLAFIPEPISESQLRLYPNIMVEDTANTINKKVGWLLH
                     GQESILVPDFNTKLQCQILGEGIGFLPDYMVREAMEKSLLVTRQIHNPRQDSRMLLAT
                     RHSASGQVTQWIKREFSLQGILTGIYQDLMHRA"
BASE COUNT          740 a          636 c          611 g          727 t
ORIGIN      
        1 gatgccagcc tggcgactat aactccgaaa agaccgatta ttgtattgcg tgcatccggc
       61 atgtttacaa tgtacgctgg tatctaacgg agtctgacac agatgcacgc aggtgtctat
      121 tttaacgtaa ttatccggaa agaacagtat attttaatgc gggagttgca tgagcggtat
      181 acgggagaaa ctatcagacc atgggagcta cagaggtgct taagtctcgt aacattataa
      241 tctgctcacc tgactggtgc tattctcacc tcaacaccaa tgcacaaata gcctggctta
      301 tcgttaataa aagttactca taccgggaat ccagtgaagc tatgaatgtc ggttcctata
      361 cactgggggc atgggtacgt cagctcaggc gggaacgtca ggataccacg tcttctgcat
      421 caccactcac atcagagtag caacgtattc atgagctgga aaagcaggtt cgtcgcctgg
      481 aggaacaaaa aacgatatta aaaagctaca gtgctcttga tatcctcccc actgaacggt
      541 ttacgatagt taccagactg agcggcgaca cacggttgtc agtctttgcg ctgctctgga
      601 tgctcattgc agcagttccg gcactggcag aatcggctca atacgattga tccggcgcga
      661 atcaaattgt atagcgaaac agtccgggtc tggaaccaga gtcggggttc cgcaggtgcg
      721 cgtgctctgg cagatatgct gattcaaaat ggtatcccga tgagcagtta tcgtgccagg
      781 aggctgatga agcatctgaa cctgagaagt gctcagcccg gaaaatatca gtacaaaaat
      841 gcccgccagg agcataccag cctgccgagt ctgcttgagt gtcggttttc tgtgctggag
      901 gccgagcagg tatggattct taaataaacc tcgagtctga atcggtaacc agctgttata
      961 ttctgaatta ttacaatagc gtcaggcctc ataattacac tgtgggttga cgccggaaga
     1021 atcagagaac agataccgtt cttactgtaa aacgtggcta atattaattg agcactgcag
     1081 tgttcagagc acaaagaaga aaaaagatgt gtgcgaatgc ctgtaatgtg ttaaaagaat
     1141 gttccgcgtt tccggtacgc ttttcgtaac taacaatgca gggtgacgta acgctggttg
     1201 tgccggaaac gcgtccctcc ccctatcttt cctgtttgga tttgccccta tatttctgga
     1261 ttatccgaca aaaccggcgt gaagtattac tctcgtcgcc cagcaccata aggataacgc
     1321 ccataaaatc atgaaacttt tcttccagat tggatacaat caggccgaaa cggtgatgaa
     1381 tattatccaa atctgatggt tcgtagcgtt ttcagccccc agattctgag ttgcctgtag
     1441 ggatgatttc aattcccgat ttgtctatcg catagagaaa aagaatttca tttctgttca
     1501 ccagaaccca tgtaacccac tccaattcgt tatttatctc actgattgcc cttcatttat
     1561 ctcattgatg caccgaaagg taaaactgcc atccggtgca tcacgctcga tgcatcaaat
     1621 cctgataaat ccccgtcagt attccttgta agctaaattc tctttttatc cactgagtga
     1681 cttgaccgct tgccgaatgc ctggttgcca acagcattcg tgaatcctga cgtgggttgt
     1741 ggatctgccg tgtcaccagc aatgactttt ccatcgcttc acgcaccata tagtctggca
     1801 gaaaaccgat tccctccccc aagatctggc actgaagctt agtattaaag tcgggaacca
     1861 gaatcgactc ttgcccgtgt aacagccagc cgactttttt gtttatagtg tttgcagtgt
     1921 cttccaccat gatgttcggg tacagacgta actgactctc agaaatgggc tcaggaataa
     1981 acgccagcgg atgagccgga gaaatcgcga acaaccaacg aatcatgccg atttcaatgt
     2041 aatcaattcc accaccgtcc agtaacgtat cgggcgcgcc aatggcgata tcagcctggt
     2101 tattaatgag tgaatcccag acgccattat acacctctgt agtaatagct atctggcagg
     2161 ttggaaagtg ttccctgagc acttgcagaa gccgagctgt atgctgaggg gtatacaaca
     2221 gttgattaat acaaatgcgc acccgggatt taattccctg agcgatggca tcaactctac
     2281 gtatgatgga gtggtaatcg ttgagcaggt cagttgcttt gcaaaaaagg tagtgcccgg
     2341 gctcggtcag ctcaatgcta cgggtagttc gggtaaaaag caccacgtca agccccattt
     2401 ccatacgctt gatagtgtaa ctaattgccg atgtggttaa gcccagttcc ttcgccgcct
     2461 tgctgaaact tctatagcgg gcagccatag taaatgccag taagttttct tccgtaaata
     2521 ttgaattcat ctttctttcc tctggcgaca atacacatac acaacatgat gattgttgaa
     2581 tgtatttgta attcttataa ccgacaatca gttttgaaaa aactaacacc gtatttacat
     2641 ttaatttgaa ggaaatcaca catctgaatt ttaattacag gcagctatcc gtgctgcaga
     2701 ccacctttat ttat
//
LOCUS       DAJOWB010000275         2704 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJOWB010000275 DAJOWB010000000
VERSION     DAJOWB010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.276"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..633)
                     /locus_tag="OHO85_004899"
     CDS             complement(<1..633)
                     /locus_tag="OHO85_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2674117.1"
                     /translation="MAGAHEFRQHGFHARRRAEIISPLAQSETVGHEAADMAAQALGL
                     SRRQVYVLIRRARQGSGLVTDLVPGQSGGGKGKGRLPEPVERVIHELLQKRFLTKQKR
                     SLAAFHREVTQVCKAQKLRVPARNTVALRIASLDPRKVIRRREGQDAARDLQGVGGEP
                     PAVTAPLEQVQIDHTVIDLIVVDDRDRQPIGRPYLTLAIDVFTRCVLGMVV"
     gene            589..714
                     /locus_tag="OHO85_004900"
                     /pseudo
     CDS             589..714
                     /locus_tag="OHO85_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229638.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(951..1799)
                     /locus_tag="OHO85_004901"
     CDS             complement(951..1799)
                     /locus_tag="OHO85_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HCQ2674118.1"
                     /translation="MNNDELATRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMREKRPLTSKQQLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPTVAIGAQAAAVHGISEQALC
                     GAPPWTDVARQLRHAIGDRPVIIFNARFDIRILKQTAAAHSDPADWLEELTVYCAMEL
                     AAGYYGATNRYGTISLACAASQAGLTWEGQAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLKI"
     gene            complement(1839..2120)
                     /locus_tag="OHO85_004902"
     CDS             complement(1839..2120)
                     /locus_tag="OHO85_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCQ2674119.1"
                     /translation="MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLA
                     GQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            complement(2117..2386)
                     /locus_tag="OHO85_004903"
     CDS             complement(2117..2386)
                     /locus_tag="OHO85_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674120.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            complement(2441..2599)
                     /locus_tag="OHO85_004904"
     CDS             complement(2441..2599)
                     /locus_tag="OHO85_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674121.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGISW"
BASE COUNT          549 a          825 c          711 g          619 t
ORIGIN      
        1 gacgaccatg ccgagcacgc agcgggtgaa cacgtcgatg gcgagggtca ggtacgggcg
       61 gccaataggt tgccggtcgc ggtcatcgac cacgatcagg tcgatgaccg tatggtctat
      121 ctgcacctgc tccagcggcg cggtcacggc aggaggctcg ccgcccacac cttgtaggtc
      181 acgagcggca tcctggcctt cccgccggcg gatgaccttg cgcgggtcaa ggctagcgat
      241 ccgtaaggcc acggtattgc gcgccggcac tcgcagtttt tgagccttgc acacctgagt
      301 gacttcgcgg tgaaaggccg ctaggctgcg cttctgcttg gtcaggaacc gcttttgcag
      361 tagctcgtgg atgacgcgct cgaccggttc cggcaagcgc cccttacctt tacctccacc
      421 ggactggccg ggcaccagat ccgtcacgag gccgctgcct tgccgggcac gccggatcag
      481 aacgtatacc tggcgccgag acaagcccag cgcctgagcc gccatatcgg ccgcttcgtg
      541 cccgaccgtc tccgactgcg ccaacggact gatgatctcc gcacgacggc gcgcatggaa
      601 accgtgctgt ctgaactcgt gtgcgcctgc catgcacgaa aggggaatgt ttcctgcccg
      661 ttgatcgcgt cactacaggg cgaagcaggc ctggcaaggt cagctatgcc ttagcgtgct
      721 ttattttccg ttttctgagg tgcccccagc cagataatcc ttttcaagtt tttcagccgt
      781 gtagcacctg ccgcctaaaa aaagccgcat ggcctccctg gttttgtctc tcacatatca
      841 tccctcatta atgctaatgt acacatacat tagcattaac atgctgacag caacaagcgg
      901 cggatttacg tctggataac gtaaatgatt gcggtataca ggccaggcgg tcagattttc
      961 agccgtgcct gttcctgcag cagttccaga tgatatgcag caatagcgtt taccacccct
     1021 gccgtcatcc gtgcgtcggc gatcgctgag tgtgcctgcc cttcccaggt cagtccggcc
     1081 tggctggcag cacaggccag tgaaatcgtg ccatagcggt tggtggcccc ataatatcct
     1141 gcagccagct ccatcgcaca atataccgtc agttcttcca gccagtcagc cggatcgcta
     1201 tgtgcggcag cagtctgttt cagaatgcgg atgtcgaacc gggcattaaa gataattact
     1261 ggtcggtccc cgattgcatg acgcagttgc cgcgccacat ctgtccatgg tggtgcgccg
     1321 cagagagcct gctcgctgat accatgcaca gcggcggcct gcgccccgat ggcgaccgtg
     1381 ggcttcagcc tggtttcaaa taccacctga ccggccgcat cggtcaggcc gatttccagg
     1441 gcttccgcgg tattatccag tcctgtcgtt tcagtatcca gaaagagcgg tgccagactc
     1501 agccagtcat atgcctgccg tgccatccgg ccactggtgc tgttaagtct ggataaaacg
     1561 gaaagtcgct ggccagccag ctgttgttta ctcgtcagag ggcgtttctc acgcatcgga
     1621 acacagtctg caattcggta tacagcgaac ctgccaccat aagtgttttt ataccactta
     1681 accggctcag caccgggggc tggtttcatt ctgaattcat cgcgcaaacg tcctttcgag
     1741 aaacacctgt cttcggcaat cgcctgcgca cgtctggtgg ccagctcatc attattcatg
     1801 gttattgcat ctcctttcct tatatccgga cagcccctct atggccactt ctgtgcagta
     1861 tgcaacacgc gcaggatata cacttttccc cactggctgt cgacctcata aaccagaaca
     1921 aaatgcgggt gaataaccag ttcccgggta ccttccacac gaccgttgcg gcctttatac
     1981 ggctgcccgg ccaggctgct ggctgaataa ccaatccgtt cgtccagttc aatggcagcc
     2041 atcaggttct gctccgcaat gtactcacga atgcgcttcc tgtcctggct ggccagcatg
     2101 gtccagaata tctccatcat ttaccggcaa ccttcctgct catttcatcc cgccatgctg
     2161 cagcagagga ttcgaccatg tcatggggaa gcacatcgcc gtgctcgagt tgctgacgtc
     2221 ctgccgcaac ctggtcacgg aaccaggtat tatgctgccg ttcaacggtc tggcgcataa
     2281 aatcccggat taactgagag ccattacggt ccatgctttt tgcagcggcc ataaaagcat
     2341 ccttcagttc agcgtcgatt cttaaactca tattaacctg tgccatttat cacctcgtca
     2401 ttactacgta tatacacagt atataacgat tcagatatcg tcaccaggat atgccgcgcc
     2461 agcggcatgg aaggcggcac gctgtggtta catgtgatac cggagtaaaa ccgccgaagc
     2521 ccggcgtcag ccggtactga ttgatagatt tcaccttacc catccccagc cctgctaagc
     2581 catacccgct ttcagccatg agagagtttc tgtgcgcggt cggagtggtc ccgacgaggg
     2641 tttacccgaa gtcggggcgt atctccgcgt tagcgggccg tcagggccgc ttacgagcgt
     2701 gtac
//
LOCUS       DAJOWB010000276         2688 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJOWB010000276 DAJOWB010000000
VERSION     DAJOWB010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.277"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            360..557
                     /locus_tag="OHO85_004905"
     CDS             360..557
                     /locus_tag="OHO85_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system protein"
                     /protein_id="HCQ2674122.1"
                     /translation="MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPH
                     DMVESSAAAWRDEMSRKVAGK"
     gene            554..736
                     /locus_tag="OHO85_004906"
     CDS             554..736
                     /locus_tag="OHO85_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCQ2674123.1"
                     /translation="MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLF
                     SLSFISVQVHDNIITV"
     gene            835..1053
                     /locus_tag="OHO85_004907"
     CDS             835..1053
                     /locus_tag="OHO85_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674124.1"
                     /translation="MDSKKMWRSNYAPPLLRILWRLGIRLPPLPFMPFWQVTLLMGGL
                     WGISWGCAMWFMYWWRRKVNRLPPWNDV"
     gene            1613..2647
                     /locus_tag="OHO85_004908"
     CDS             1613..2647
                     /locus_tag="OHO85_004908"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCQ2674125.1"
                     /translation="MQSASKLIYRGKLLGSLPTVGEIHKEIAVSEETVTWISLQRDII
                     ANILLGKDPRLLVIVGPCSIHDVQAAVDYAKRLFVLQNKYLSKMYIVMRTYFEKPRTR
                     KGWKGIMHDPDLNGSYDVEKGIRYARQCLSSITTMRVATATEFLDPFLTPYIADLICW
                     GAIGARTTESQTHRQLASGLHCPVGFKNSTDGNINLAIDAIIAAREQHIVYMTSLTNS
                     ISTLLTDGNLHGHLILRGGREPNYGLSDITKAVKLMHDEGINHRLIIDCSHGNSGKVA
                     KRQISVARQVIDNRKKIPGYVAGIMVESFLQDGKQSDSFPLEYGQSVTDECISWQQTE
                     QLLSTLAAQL"
BASE COUNT          731 a          571 c          668 g          718 t
ORIGIN      
        1 gttaaagtgt catatcgttt acataaagtc aagatatatc agtgtggcaa gcctggcagg
       61 attctctagg gaagggcagg catactcagt gcatagctga tgcacggatg acggcagggg
      121 ggaggaggct ggtgactttc ggacgactgt agcggcttac atcgggcttc ggcggtatca
      181 tatgaaacag cggagtgccg ccttccatgc cgctggcgcg gcatatccta gtgacgatat
      241 ctgaatcgtt atatactgtg tatatacgtg gtaatgacgg ggtgataaat ggcacaggtt
      301 aatatgagtt taagaattga cgctgaactg aaggatgctt ttatggccct gcaaaaagca
      361 tggaccgtaa tggctctcag ttaatccggg attttatgcg ccagaccgtg gaacggcagc
      421 ataatacctg gttccgtgac caggttgagg caggacgtca gcaactcgag cgcggcgatg
      481 tgcttcccca tgacatggtc gaatcttccg ctgctgcatg gcgggatgaa atgagcagga
      541 aagtcgccgg taaatgatgg agatattctg gaccatgctg gccagccagg acaggaagcg
      601 cattcgtgag tacattgcgg agcagaacct gatagccgcc attgaactgg acgaacggat
      661 tggttgttca gccagcctcc tgttctccct ttcttttatc agtgtacagg tgcatgacaa
      721 cataatcacg gtatgataat aataatcaat aacaataagt tgtgtcacgt ttacatcatg
      781 gaacggaggt gctcctgtga cttttgagca aaaaaagccc gggcaattgc cctgatggac
      841 agcaaaaaga tgtggcggag taattatgct cctcctctgc tgcgtattct ctggcggctg
      901 ggtatccggc ttccgccatt gccctttatg ccgttctggc aggttacgct gctgatgggg
      961 ggcttatggg gtatttcctg gggatgtgca atgtggttta tgtactggtg gcgcaggaaa
     1021 gttaaccggc tgccaccatg gaatgatgtg taaaccgtgc cggcatgttt ttacggggtg
     1081 atgctttcat taacctgtac acgaacttct attcctgcat cattacaggc ctacagccac
     1141 tacgccactt ccagcggatc gccctccctg cgtacctccc cgccttcttt attctataac
     1201 tgcagacagg tgctgccgtc gaggcgcacc acaaaatccc cacggcaggc ctgatatgtc
     1261 atgccgttca gctccaccgg cagatcagtg gcagaagtgc catctgcggg cagacctgat
     1321 gccgggacca gcggcagtac gacgacttct gcagtacgct gttctgccag cagagaggct
     1381 gtgccacggc ccggtcagct tttgtgagtt ccacagctag ccgcagctca gggattaaga
     1441 ttcaggtaat ttacggaaaa caagtgattt ccgtacggga gagaaggcct aacgtatatt
     1501 atctcagaaa tgactatgaa tccccatata ttgccaatta tacagtaaat gattatcatt
     1561 atcatctaat gtgtgcaact gatggtctga aatagcgctt ttaggagaca agatgcagtc
     1621 agctagtaaa ttaatctacc gcgggaagtt gcttggcagt ttacccactg tgggcgagat
     1681 tcataaagaa atcgcagtat cggaagaaac cgtaacatgg atctctctgc aacgagatat
     1741 tatagcgaat attttgctgg gaaaagatcc tcgcttactt gtgattgttg gtccatgctc
     1801 aatccatgac gttcaggccg cagttgatta tgcgaagcga ttgttcgtat tacagaataa
     1861 gtacttatcg aaaatgtaca ttgtaatgcg cacttatttt gagaaacctc gaacccgtaa
     1921 aggctggaag ggaataatgc acgatcctga tctaaatgga agttatgatg ttgagaaagg
     1981 gatacgatat gctcgccagt gtttgtcttc aatcacaacg atgcgtgtcg caaccgctac
     2041 agaattcctc gatccttttc ttactcccta tattgctgat ttaatatgtt ggggtgctat
     2101 tggtgccaga accactgaat cacaaacgca tcgtcaactt gcgtctggtt tacattgtcc
     2161 agttggattt aaaaacagta ctgatggaaa tattaactta gctattgacg ctattattgc
     2221 agcccgtgaa caacatattg tatatatgac atctttaaca aatagtatat ctacactatt
     2281 aaccgatggc aatctacatg gacatctaat attacgtgga ggtagagaac ctaactatgg
     2341 tttgtctgat ataactaagg cagtgaaatt aatgcatgat gagggcatta accatcgtct
     2401 gataattgat tgtagccatg gtaatagcgg taaagtggct aaacggcaaa tttccgttgc
     2461 acgtcaagtt atagataatc ggaaaaaaat accaggatat gtagctggca ttatggtgga
     2521 aagcttttta caagatggaa aacaatccga tagttttccg ctagagtatg gtcagtctgt
     2581 tactgatgaa tgcatatcat ggcaacagac agaacaatta ttatcaactc tggcagcaca
     2641 gctttaactt caactattgc acatatattg cttttaaagg gggaaaaa
//
LOCUS       DAJOWB010000277         2664 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJOWB010000277 DAJOWB010000000
VERSION     DAJOWB010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.278"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>2664
                     /locus_tag="OHO85_004909"
     CDS             <1..>2664
                     /locus_tag="OHO85_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCQ2674126.1"
                     /translation="FARATSGDINEIVAPKQEFLATLPDGPVKNNVLNQLAAIEEKRI
                     KTPAENIRFKLNSRWFDRSVILEFLQENGYPDLRYVQSVQLEGDEMVSDTYHGGDGLF
                     VGHRYGVVQRKDKETGEIRYEWDRKSGENATGFPAQLEKYLNGARIGGKDSATANGYR
                     EQMALLEDQFNKWIKTHDRYDELVAKYNDVFNSNIPYEHSGDPLGLKGLSGKRQPFDY
                     QNSEVRRLSEDGRGILGFGTGLGKTTTALALEAFNYENGRSTRTAYVVPKSVLENWYY
                     EAKEFLSEEAFSNYLFVGLDVLMDGDQIRQVPVLNENGKPVLGTDGTPVMRDALKLAD
                     EATITARMNAIPHSNYRAVVFTKEQYARIPLRDDTVDEHAQDMLYDFVAAGRVASAMD
                     SDSHRKEAARRRVLSEYSDTGTEKAEKYPYFEDMGFDSVIADEGHNYRNSYKNGREAS
                     QLAYLPTSAVAQSARDMAIKNAYLMKKNGGRGPVLLTATPVVNTPIDAYNMLSHVLPK
                     EYWQNMGIYGPDDFVKFFGKTRLETVQKISGEVEEKMALVGFENLDALRGIFHRWVTL
                     KTAEDVKDTVEIPELDEHQQDAPLTEEQLAAYEELRQQAEAAAKANNGVTTSVNEDGV
                     IEHEKARPIFSIIRDMDRVCTDMDLYYRRITYRFLPEYADAVQQLADSLPKQATSEDD
                     DSDDSITQQSQYSLIDKGEFIQLQVPEAFEQEVNKRLARFGIDEQTVTHPVTPKYAKL
                     IATLKEFFPEGKQIIFTDEKTQHQKLKRIICNALNLEPSKVGILNAQTVAEAGKTGKK
                     LKAVKPPKELPDEPTDAQIAKYNEQMALYDAYIAQQNEMSLGGLEKIAADFQEGRTPI
                     IICNKKAEVGINLHRGTTDIHHLTLPWTPAS"
BASE COUNT          729 a          664 c          741 g          530 t
ORIGIN      
        1 gttcgcccgc gccaccagcg gcgacattaa cgagatagtg gcaccaaaac aggaattcct
       61 tgccacactc cccgacggtc cagtaaagaa caacgtcctt aatcagctgg cagcgatcga
      121 agagaagcgc atcaagacgc cagcagagaa tatccgcttt aagctcaata gccgttggtt
      181 cgaccgttcc gtcattctgg aatttttgca ggaaaacggc tatccggatc ttcgctatgt
      241 gcagtcagtg cagctggaag gcgacgaaat ggtttctgac acctatcacg gtggtgatgg
      301 tctgttcgtc gggcaccgat acggtgtcgt ccagcgcaag gataaagaaa caggcgagat
      361 ccgctacgag tgggaccgta aatcaggtga aaacgcgacc gggttcccgg cacagctgga
      421 aaagtatctc aatggtgcgc gtatcggtgg caaagatagc gcgacggcga acggctaccg
      481 cgagcagatg gcactgcttg aggaccagtt caataagtgg atcaagacgc acgatcgcta
      541 cgatgagctg gttgccaaat acaacgatgt tttcaatagc aatattccgt atgaacactc
      601 tggcgatccg cttgggttga agggattaag cggtaagcgc cagccatttg attaccagaa
      661 tagcgaggtg agacgactgt ccgaagatgg gcgcggcatc ctgggcttcg gcaccgggct
      721 gggtaaaacg acgaccgcgc tggcgcttga ggcgttcaac tatgagaacg gtcgctccac
      781 ccgtactgcg tatgtagtgc ctaaatcagt gctggaaaac tggtattacg aagcaaaaga
      841 gttcctgagt gaagaggcat tcagtaacta cctgttcgtc ggtcttgatg tgctgatgga
      901 tggcgatcag attcgccagg tgccggtgct caatgagaac ggtaaacctg ttcttggtac
      961 tgatggcact ccagttatgc gcgatgctct taagctggca gatgaagcca ctatcacggc
     1021 gcggatgaac gcgatcccgc actcaaatta ccgtgcagtc gtgtttacca aagaacaata
     1081 cgcccgcatt ccgctacgtg atgacaccgt agatgagcat gcacaggata tgctttatga
     1141 cttcgttgca gccgggcgcg tagccagcgc aatggactcc gactcccacc gcaaagaggc
     1201 cgcgcgtcgc cgggtattgt cggagtattc agataccggc accgaaaaag cagagaagta
     1261 tccgtacttt gaggatatgg gcttcgatag tgtgatcgcc gacgaaggtc acaactaccg
     1321 caatagctat aaaaatggtc gcgaagcgtc acaactggcc tatctgccca ccagcgcggt
     1381 ggcgcaatcg gcgcgagata tggcaattaa aaacgcgtac ctgatgaaaa agaatggcgg
     1441 gcgcgggccg gttctcctga ctgcaacgcc agtcgttaac accccgatcg atgcatacaa
     1501 catgctttct catgttctgc cgaaggaata ctggcagaac atggggatct acggtcctga
     1561 tgacttcgtt aaattcttcg gcaagaccag gctggaaacg gtacagaaaa ttagcggtga
     1621 agttgaagaa aaaatggcgc tggtgggctt tgaaaacctt gatgcgctgc gcggtatatt
     1681 ccatcgctgg gtaacgctta aaacggcgga agacgttaag gataccgtgg agatcccgga
     1741 gctggacgaa caccagcagg atgcaccact tactgaagaa caactggcgg cgtatgaaga
     1801 attgcgtcag caggcggaag cggcggccaa agccaacaat ggcgtaacga cctcggtcaa
     1861 tgaagacggc gtgattgagc acgagaaagc ccgtccgatc ttctcaataa tcagggatat
     1921 ggaccgcgta tgtactgaca tggacctgta ctatcgccgg atcacctatc gtttcctgcc
     1981 ggagtacgcc gatgcggtgc agcagctggc ggacagtttg cctaaacaag ccaccagcga
     2041 agacgacgac agtgatgatt caatcacgca gcaatcgcaa tactccctga tagataaggg
     2101 cgagtttatt cagttgcagg ttccggaagc gttcgagcag gaagtgaata agcgcctggc
     2161 caggtttggc attgacgaac agaccgtaac tcaccccgtt acgccgaaat acgcgaagct
     2221 gatcgccacg ctgaaggagt ttttcccgga aggtaagcaa atcatcttca ccgatgaaaa
     2281 aacgcagcac cagaagctca agcgcattat ctgcaatgct ctaaaccttg aaccttcaaa
     2341 ggtggggatc ctgaatgctc agacggttgc cgaggcaggt aaaaccggta agaaactgaa
     2401 agcggttaaa ccgccgaaag agctaccgga tgaaccaaca gatgcacaga tagcgaaata
     2461 caacgagcaa atggctctgt atgacgccta tatcgcgcag caaaatgaaa tgtcgttggg
     2521 cggtctggaa aagattgcag ccgacttcca ggagggccgg actccgatca tcatctgcaa
     2581 caaaaaggca gaggtgggta tcaacctgca tcgaggaacg actgacatcc atcatctgac
     2641 gttgccatgg acaccagcca gcat
//
LOCUS       DAJOWB010000278         2659 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJOWB010000278 DAJOWB010000000
VERSION     DAJOWB010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.279"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..101
                     /locus_tag="OHO85_004910"
     CDS             <1..101
                     /locus_tag="OHO85_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCQ2674127.1"
                     /translation="LARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            117..395
                     /locus_tag="OHO85_004911"
                     /pseudo
     CDS             117..395
                     /locus_tag="OHO85_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532129.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
     gene            498..1163
                     /locus_tag="OHO85_004912"
     CDS             498..1163
                     /locus_tag="OHO85_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674128.1"
                     /translation="MGSVTKKPSPVVIIKKKRRILSHQDDAVQAVPDDIKPAPVMEFK
                     TDSLPPEIPATVAGSPEQQKKTGRKKKKKKKNNRWNHEFIRHSLDCVKKTFPQLRAEE
                     GGYHAFKIGISKDVMAFISASPECGLTVKEWNCAVRMITGGYKYLQRISVAGTPRYGL
                     DGKVHGTVTEEEALYAQAKLEKHTQRYNARMKETEDARCNQPSKTVTPDSNLKDACIH
                     NGA"
     gene            1221..1511
                     /locus_tag="OHO85_004913"
     CDS             1221..1511
                     /locus_tag="OHO85_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674129.1"
                     /translation="MITVIISEVDGWREWKHRARTKDAQTAIIRAMNKHFPRSYNFIP
                     DDIDNAPVLFAAVTRTPNVKITGHIWKPMWNRGICWNVKGPPVIITLIQGGA"
     gene            complement(2189..>2659)
                     /locus_tag="OHO85_004914"
     CDS             complement(2189..>2659)
                     /locus_tag="OHO85_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCQ2674130.1"
                     /translation="ELSASILEDVAVVPQKAVLVQGTIRDNILYGTDDILPDDELFCI
                     LNSLGLIKEDCGEHFLAFLDRDINPLGSGLSGGEIQRLCIARALARKKKIIVLDEPTA
                     SIGEELAIKIITYIRQHCPTVIITTHNPRILELADHFFDGMSKSCVIAGEGRDQ"
BASE COUNT          819 a          547 c          596 g          697 t
ORIGIN      
        1 acctggcacg gctgggacgg aagtcgctgt cgttctcaaa atcggtggag ctgcatgaca
       61 aagtcatcgg gcattatctg aacataaaac actatcaata agttggagtc attacccatg
      121 caggaatcgt cattcatggt aatgttgata tcggtgagaa tgtcattata cggcagaata
      181 caaccattgg tgaaaaggtt tctgacagta gggaaaacta tattgttatt ggtgataatg
      241 tcgatattgg tgcccataca tgtattattg ggttaaatgt gaaaatcgga agtaatgtta
      301 aaatcggggc aatgtctttt attatggaag atgtacctga taactgtact tatgtcacca
      361 ggaaggaatc cagagtgata attcgcgaac agtaatgaaa tacatgttat tattcttctg
      421 ttggtgactt taccgactca gatatatact atctgtatgt cgtgcattat atattaacga
      481 tacgcagagg tgtgtatatg ggcagtgtta caaagaaacc gtcacctgtt gttattatta
      541 agaaaaaaag acgaattctt tctcatcagg atgatgctgt acaggcagtt cctgatgata
      601 taaaaccagc gccagtaatg gaatttaaaa ccgactcatt acctcctgaa ataccagcca
      661 cagtggccgg ttccccggaa caacagaaaa aaacaggacg taagaagaag aaaaaaaaga
      721 agaacaatag atggaatcat gagtttatcc gacacagtct ggactgtgtt aaaaaaacat
      781 tccctcagtt acgggctgaa gaggggggat atcatgcatt taaaataggc atctcaaagg
      841 atgtcatggc ttttatttcg gccagtcctg agtgtggact aactgtaaaa gaatggaatt
      901 gtgctgtaag gatgattact ggcggttaca aatatctgca gcgtatatca gtcgcaggta
      961 ctccgcgtta tggtctggat ggcaaagttc atggaactgt aacggaggaa gaagcgctgt
     1021 atgcgcaggc aaaacttgaa aaacacacgc agcgttataa cgccaggatg aaagagacag
     1081 aagatgctcg ttgcaaccaa ccatcgaaaa ctgtcacacc ggacagcaat ttgaaggacg
     1141 cctgcattca taacggggca taattatgtg aaatgctgtc ccgctatgga atcaaaattg
     1201 agtaacgaca cgaggttttg atgattactg ttattatttc tgaggtggat ggctggcgtg
     1261 aatggaaaca ccgcgcccga acaaaggatg cacagacagc catcatccgg gctatgaata
     1321 aacattttcc acgatcttac aattttatcc ctgatgatat tgataatgca cctgtattat
     1381 ttgcagctgt aacacgtaca ccgaacgtga aaataaccgg tcatatctgg aaacctatgt
     1441 ggaacagggg aatttgctgg aacgtcaaag gtccgccagt aatcatcacg ctcatccagg
     1501 ggggcgcttg aaattcggaa aataaaccac gttaagacta tttggggttc taagccgaaa
     1561 acctagaaaa ttccgtaacc aaagccagta attgacagat ttgcatgacg ttgaataggt
     1621 gacgggttat gtgacgaaat ctgatgcaga aatcgttgtt tcagtgacag tcactcaatc
     1681 ggtcgtttat gtgacaaccc acgccgttac tggtcgcgga aaaatccagg tttcggggtt
     1741 tgaggctcag tggaacaata accgccgtta agcggtcatt ttaaatagcc aaaaatcaac
     1801 cagccaattt acatcgtgaa tctgcgttct tggctatctg ttttgggggg ggccaagttg
     1861 gctttactca tcctggccgt ttgcggaaca aacgacgttg ttgatctcaa cagattgatt
     1921 tacctgttat tgaccattca attgaaccgc ttaaccgcct tttgagttcc actactcccc
     1981 aaaccccaaa tagctgtcta gcgcgaaaaa tcactgcccg taacgtatca tatggctggt
     2041 acgaataaat tctcacttac tgcaccataa acgtacacgt caaaaccttc caaacatgct
     2101 tcaaaaatca acaaattcgc tgtatcggaa atttcggcgt ttccggcgta caaccccgat
     2161 tagatataaa tgaattaacg tgtgtggttt attggtctct gccttctcca gcaatgacgc
     2221 aagactttga cataccatca aaaaaatgat cggcgagctc cagaattcgt ggattatgag
     2281 ttgtaataat aacggtaggg caatgctgcc ggatataggt aatgatcttt atcgcgagtt
     2341 cttcgccaat agaggccgtg ggttcatcaa gtacgataat ctttttctta cgggctaggg
     2401 cgcgggctat gcataatcgc tggatctctc caccactcaa accactaccc agaggattaa
     2461 tatcccggtc taggaaagca aggaaatgtt ctccgcagtc ctctttaatt aaccctaatg
     2521 aattcaggat gcaaaacagt tcatcatcgg gaagaatgtc atcggttcca tacagaatat
     2581 tatcacgaat agttccctgc accagaacgg ctttttgagg cacaacagca acgtcctcca
     2641 ggatagacgc actcaactc
//
LOCUS       DAJOWB010000279         2659 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJOWB010000279 DAJOWB010000000
VERSION     DAJOWB010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.280"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(82..273)
                     /locus_tag="OHO85_004915"
     CDS             complement(82..273)
                     /locus_tag="OHO85_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HCQ2674131.1"
                     /translation="MNPIFALILTVFLVSGEPVDIAVSVHRTMQECMAAATEQKIPGS
                     CYPVDKVIHQDNNEIPAEF"
     gene            complement(270..458)
                     /gene="dicB"
                     /locus_tag="OHO85_004916"
                     /gene_synonym="ftsT"
     CDS             complement(270..458)
                     /gene="dicB"
                     /locus_tag="OHO85_004916"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HCQ2674132.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKV
                     CIVSMLARLRLMPKGCAQ"
     gene            1020..1295
                     /locus_tag="OHO85_004917"
     CDS             1020..1295
                     /locus_tag="OHO85_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674133.1"
                     /translation="MGFDVNETLNSLIRKINDFDKTLQHAAARSDITLLAISYLASAM
                     NKDETVRKNLVDYIDSLQPGTFNPESFNHEKEHVKSVINSLVLNQKN"
     gene            complement(1264..1593)
                     /locus_tag="OHO85_004918"
     CDS             complement(1264..1593)
                     /locus_tag="OHO85_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674134.1"
                     /translation="MEFKDLPMQFQEMAANIVRSQLATLDLSTVEKETIDTISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLDLMDIINKGFGLLMEKKGIRIEPLENCFVTRSINS
                     FDSKQEN"
     gene            complement(1616..1834)
                     /locus_tag="OHO85_004919"
     CDS             complement(1616..1834)
                     /locus_tag="OHO85_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674135.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANEARAKQAE
                     NLSALEQDITRLEKAAALSIARMITYPR"
     gene            complement(1906..2205)
                     /locus_tag="OHO85_004920"
     CDS             complement(1906..2205)
                     /locus_tag="OHO85_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674136.1"
                     /translation="MALYIDISAIAGQVRIIRAVTKRYAPLLQKVSGECTEDIVNDFI
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDFATTFWARKLDCTHNQFPLE"
     gene            complement(2546..>2659)
                     /locus_tag="OHO85_004921"
     CDS             complement(2546..>2659)
                     /locus_tag="OHO85_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCQ2674137.1"
                     /translation="IDGKLVYLSPLNPRYQSSLLTPECKIVAVAKGVQFNL"
BASE COUNT          698 a          611 c          579 g          771 t
ORIGIN      
        1 tcatcacaga tttaagggtg aacaaatctc tgccattgct gacatataag aatgaaactg
       61 gatatttatt acggtgctgt tttaaaattc tgccgggatt tcgttattat cctggtgaat
      121 aactttatcg accggataac agctgcctgg aattttctgt tcggttgctg ctgccataca
      181 ttcctgcatt gttctgtgaa cactgactgc aatatcaact ggctctccgg aaacaagaaa
      241 aaccgtcaga ataagtgcaa atattggatt cattgtgcac atccttttgg catcagacgt
      301 aaacgggcca gcattgaaac aatgcatact ttatttaata actcccgttc gtgttttctt
      361 ttgttaatgg catcttcagt aaatacagga ttactgatag tgacaccaat ttcaaaacaa
      421 ccttcagacg tattaacgtt tggtaataac gttttcatta tcgcgccctc aacaatgagt
      481 tttgtgatgc ggtgcctggt gcctccaggt gacgttaacc agttaacaat taacgccgga
      541 tacagagaat ccacccataa cactgttttt ggttttaact gttccgcgtg cgcttagccg
      601 cattcaccgc atcacaaaat tcactttaaa aagggcggca gagcagtcac ggagtaaaac
      661 tgataccgcc aaacgtcacc agaaaattga taacagaggg cgttgcagcg gggttatcac
      721 ttaagcgtat ggtcaacctg acaacccggt gtcctcaacg gggaaggaat aaccccgcca
      781 tacttaccgc cgcgccattt cgcggagtgc cacaaccgga agcgcacggt cgacgaaaat
      841 ttaacgacag gctatctatg aaccaacaac ttcgccgtgc gctttcgcgt tatgccctga
      901 cttttcaggg agatatcctt tcagtaaact gtcagtgccg gatgctcacc cgtgtccggc
      961 gcacgcactc cacctcaccc gtggagaact ccttaattac taaccctcag gaggatgaaa
     1021 tggggtttga tgtcaatgaa actcttaact ctcttatacg gaaaattaat gattttgata
     1081 aaacactaca gcatgctgcg gcgcgtagtg atataacatt gttagcaatt tcataccttg
     1141 catctgccat gaataaagat gaaacggtac gaaagaatct cgttgattat atcgactcgc
     1201 ttcagccggg cactttcaac cctgagagct tcaatcatga gaaagagcat gttaaatctg
     1261 taattaattc tcttgttttg aatcaaaaga attgatgctt cttgttacga agcaattttc
     1321 aaggggttct atccgaatcc ctttcttttc cattaacaag ccaaacccct tattaatgat
     1381 gtccattaga tccaggaagt atttttcatg taaatcctgg ttatcagaga gctgcttctc
     1441 ttcgtacaga ccgataaagg cacgacgcac gttaccggat atagtatcga tggtttcttt
     1501 ttctacggta ctcaggtcaa gagtcgccag ttgggaacga actatattcg ctgccatttc
     1561 ctggaattgc attggtaaat ctttaaattc cattattagc ctcgttggtt agctattaac
     1621 gcgggtatgt aatcattctg gcaatgctta atgccgctgc tttttccagc ctggtgatat
     1681 cctgctccag agcggacaga ttttcagcct gcttagccct ggcttcattg gcccatttca
     1741 gatcctgcgc tgcattaatt ttctggcgca tccactcata aagttcatca tcggtatagt
     1801 ctggcgcgat gatgacgggt tctcgtttct gcatgtcggc tccttgtggt tagcgttgcc
     1861 tgcttttaac cacgtcaggc gaggtggtat cctctgaggg gtctgttact cgagaggaaa
     1921 ttggttatga gtacaatcaa gttttcttgc ccagaatgtg gtggcgaagt ctttgacaca
     1981 tcctttaagc cgcagggctc tgacagtttc gcgggagcca tctgcaaaaa ttgtggtcac
     2041 cttgtaactg aagatgagtc ctcgcagttc gatgatgaaa tcgttgacaa tatcttcggt
     2101 gcactcacca gagactttct gaagtaaagg cgcataccgc ttagttaccg ctctgataat
     2161 tcttacctgt ccggcaatgg cgctgatgtc aatataaagc gccattgctg cttctttgcc
     2221 gatcccggga tgccttccat tctgatgttt gacttcgccc actgagaaat cctctgtttc
     2281 cccttaacgc cggggtagcg gaacaaaaac ctgctgcata gttattaaag ttgaaccctg
     2341 ccgtcatgtt cttacgcctc gggctggcta cttaacccct gaccactgcc tggtaactcg
     2401 aagtattgcc cggcgttcta tggggcgggg tgggttggta tgcaataaat ctaaaagtat
     2461 ttagttttat agtcaagggg ggatctaaat taatttaaaa aaaaaggtcg gcataaccga
     2521 ccttaagttc aaggacatag cagggttata ggttgaactg cacacctttt gctacggcaa
     2581 caattttaca ctctggcgta agcaaggatg attgatagcg cggattgaga gggcttaaat
     2641 acacaagttt tccatcaat
//
LOCUS       DAJOWB010000280         2656 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJOWB010000280 DAJOWB010000000
VERSION     DAJOWB010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.281"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            155..1768
                     /gene="mppA"
                     /locus_tag="OHO85_004922"
     CDS             155..1768
                     /gene="mppA"
                     /locus_tag="OHO85_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCQ2674138.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1819..>2656)
                     /gene="ynaI"
                     /locus_tag="OHO85_004923"
     CDS             complement(1819..>2656)
                     /gene="ynaI"
                     /locus_tag="OHO85_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCQ2674139.1"
                     /translation="ENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKG
                     RDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNF
                     FSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSIS
                     VENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFAD
                     SSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQ
                     GR"
BASE COUNT          761 a          591 c          634 g          670 t
ORIGIN      
        1 ggcgtcgatt atttacacta aatctgattt aatatattga tacataaaac attttatgct
       61 tcctgtgtca cttttttgat ggaagttgtt tgcatttctt taaggcgaaa caaataatta
      121 cgcatcaatt ttaatgtcgg ttagagggaa acttatgaag cactctgttt cagtcacgtg
      181 ttgtgcgctg ttggtcagca gcatttctct ttcgtatgct gcagaagttc cgagcggcac
      241 agtactggca gagaagcagg agctggtgcg ccacattaaa gatgagcctg cgtcgctgga
      301 tcccgctaaa gccgtgggcc tgccagagat tcaggtcatt cgcgatctgt ttgaaggact
      361 ggtgaatcag aacgagaaag gggagattgt ccccggcgtt gcgactcagt ggaaaagtaa
      421 tgacaaccgt atctggactt ttaccctgcg cgataacgca aaatgggcgg atggcacacc
      481 ggtaacggcg caagattttg tctacagctg gcaacgtctg gtggacccga aaactctatc
      541 gccatttgca tggtttgccg cgctggcggg aatcaacaac gcacaggcga ttattgatgg
      601 taaagctacg cctgaccagc ttggcgtcac cgcagttgat gcccatactt tgaaaattca
      661 gcttgataaa ccgttgccgt ggtttgtgaa tttaaccgct aactttgcct tcttcccggt
      721 gcaaaaagcc aacgtagaaa gcggtaaaga gtggacgaaa cccggaaatc tgattggcaa
      781 tggcgcttat gttcttaaag agcgcgtagt caatgaaaaa ctggtcgtgg taccgaatac
      841 ccattattgg gataacgcca aaacggtact gcaaaaagtg accttcctgc caattaatca
      901 ggaatccgca gccactaagc gttacctcgc gggggatatt gatatcaccg aatccttccc
      961 gaaaaatatg tatcagaagc tgttgaagga tattccgggg caggtttata cgccgccgca
     1021 gctcgggacc tattattatg cgtttaacac gcaaaaaggg ccgacggcag atcagcgcgt
     1081 tcgtctggca ttaagtatga cgatagatcg ccgcctgatg accgaaaaag tattagggac
     1141 gggcgaaaag ccagcgtggc attttacacc agatgttacc gccggattta cgccggaacc
     1201 ttcgccgttt gaacaaatga gtcaggaaga actgaatgcg caggcaaaaa ctttgttgag
     1261 cgcagctggt tatggtccgc aaaaaccgct gaagctgacg cttttgtata acacttcaga
     1321 aaaccatcaa aaaattgcga ttgctgtagc atcgatgtgg aaaaagaacc ttggcgtaga
     1381 tgttaaattg caaaatcagg aatggaaaac ctatatcgat agccgtaaca ccggcaattt
     1441 tgatgttatc cgcgcctcat gggtggggga ttataatgaa ccctccactt tcctgacatt
     1501 attaacgtca acgcattcag gaaatatttc acgctttaac aatccggcat atgacaaagt
     1561 tctggcccag gcatcgacgg aaaataccgt taaagcgcgt aatgccgatt acaacgcggc
     1621 agaaaaaatc ctcatggagc aagcaccgat tgcaccaatt tatcaatata ccaatggacg
     1681 attaatcaaa ccgtggctga aaggttatcc cattaataac cctgaagatg tggcgtacag
     1741 tcggactatg tatattgtga agcattgatg tgatgggaac tggcgttacc cttgtggata
     1801 acgccagtga tgtctgattt agcgaccctg ttccggcggt gtaatgttat ccatatacag
     1861 cgtctggctg gggaaggcaa agtccgcgcc gtgtgactgt acaatatcga taatcttcaa
     1921 ataaacgtct tgctgtgcag caagccattc agcccatacc gtggttttgg taaagcaata
     1981 aaccataata ttcaatgacg agtcggcaaa ctggttgaaa taaaccagta aggtttgtcg
     2041 ctggtcgatg gccgggtgat ttttcagcat ctcacgtacg gcttcgacaa taacgcccac
     2101 ttttgccgca tcctcataac gtaaaccaat ggtcgtggta atgcggcggt tggtcattcg
     2161 tcctgggttt tctacgctga tcgacgaaaa cagcgagttc ggtacgtaca atggacgatt
     2221 atcaaaggtc gtaattttgg taattcgcca gccaatttcc gctactgtac cttcgatatt
     2281 tctgtccggt gaacggatcc agtcgccaat actgaaagga cggtcgaaat agagcatgat
     2341 cccggaaaag aaattactca gaatatcttt accggccata ccgacagcca gaccaccaat
     2401 accaccaaag gtcagcaagc cagaaaggct catgccgaaa tgttcgccat aaagcagaac
     2461 aagcaccaca ataatggtga ttttgataat gcgcgacata atccgcgcac tggtgatatc
     2521 gcgacctttt ttaatctgct gtttttcaaa ctgattaatc agcaaaaata gcttaatcgt
     2581 cagaataacc gcaatcaggg acgtacagat aaaatcgata acgcctgggg tgataaattt
     2641 gagtttatag ttttct
//
LOCUS       DAJOWB010000281         2630 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJOWB010000281 DAJOWB010000000
VERSION     DAJOWB010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.283"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            98..607
                     /locus_tag="OHO85_004924"
     CDS             98..607
                     /locus_tag="OHO85_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCQ2674140.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            604..2022
                     /gene="phrB"
                     /locus_tag="OHO85_004925"
     CDS             604..2022
                     /gene="phrB"
                     /locus_tag="OHO85_004925"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCQ2674141.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMISASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(2064..>2630)
                     /gene="dtpD"
                     /locus_tag="OHO85_004926"
     CDS             complement(2064..>2630)
                     /gene="dtpD"
                     /locus_tag="OHO85_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCQ2674142.1"
                     /translation="YTVPTAMFQSINAFAVMLCGVFLAWVVKESVAGNRTVRIWGKFA
                     LGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAELFIDPVAMSQITRIEI
                     PGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINYSINAYIEVFDQITWG
                     ALACVGLVQMIWLYQALKFRNRALALES"
BASE COUNT          631 a          677 c          704 g          618 t
ORIGIN      
        1 atttttacca acaaaatact tccccgcaaa aactctgccc cataaaattc atttaccatc
       61 gtctacactt actcctttat accgttcagg agcagtaatg aatctacaac gatttgatga
      121 cagcacccta atccgtatct ttgcccttca tgagttacat cgactgaaag aacatggctt
      181 aacgcgcggg gcgcttctcg attatcacag ccgctataaa ctcgtctttc tggcgcattc
      241 tcagccggag taccgcaaac ttggcccgtt cgtggctgat attcaccagt ggcaaaatct
      301 ggatgactat tacaaccagt accgccaacg cgtagttgtt ttgctttctc accccgccaa
      361 cccgcgcgat cacaccaatg ttttgatgca cgttcagggt tattttcgcc cgcatattga
      421 ttccacagaa cgccagcagc tggctgcgct tatcgacagt tatcgccgtg gcgagcaacc
      481 acttcttgcg ccgctgatgc gtatcaaaca ctatatggcg ctttatcctg acgcctggct
      541 ttcagggcag cgttatttcg aactttggcc gcgtgtgatt aacttgcgcc attcaggagt
      601 tttatgacta cccatctggt ctggtttcgc caggatttac gtctgcacga taatctcgca
      661 ctggctgccg cctgccgcaa ttcgtctgca cgcgtgctgg cgttgtatat cgctacacca
      721 cgccagtggg cgacgcataa catgtcgccg cgtcaggctg aacttatcaa tgctcaactg
      781 aatgggctac aaatagcgct tgcggaaaaa ggtattcctt tattgttccg tgaagtggat
      841 gactttgtcg ccagtgtcga aatagttaaa caggtgtgcg cggaaaacag cgttacccac
      901 ctgttttata actatcagta tgaagtgaat gagcgggcgc gggatgtgga agttgaaaga
      961 gcgctgcgta acgtggtgtg tgaaggattt gatgacagcg tgatcctgcc gcctggcgcg
     1021 gtgatgaccg gtaatcacga gatgtacaaa gtctttacgc cttttaagaa tgcctggctg
     1081 aaacggctgc gggaagggat gccggagtgc gtcgctgcgc caaaagttcg tagtagcgga
     1141 tcgatagagc cctcgccatc cattacgctg aattatcctc gtcagtcttt cgatactgcg
     1201 catttcccgg tggaagaaaa agcggcgatt gcgcaattac gccagttttg ccagaacggt
     1261 gccggagaat atgagcaaca acgagatttt ccggcagtgg aaggcaccag tcgtttgtcc
     1321 gccagcctgg caacgggcgg gttatcgcct cgccagtgtt tgcatcgctt gttggcggaa
     1381 cagccgcagg cgctggacgg tggggccggt agtgtctggc ttagtgagct gatctggcgc
     1441 gagttctacc gtcatctgat gacgtattac ccctcgttgt gtaaacattg tccgtttatt
     1501 gcctggacgg atcgtgtgca gtggcagagc aatcccgcac atttacaggc ctggcaggaa
     1561 ggcaaaacgg gatacccgat tgtcgatgcc gccatgcgtc agcttaacag cactggctgg
     1621 atgcataacc ggctacggat gatttcagcc agttttctgg tgaaagattt gttgatcgac
     1681 tggcgcgaag gcgagcgata tttcatgtcg cagctgattg atggtgattt ggcagccaat
     1741 aacggtggct ggcagtgggc ggcgtctacg ggtactgatg cagcgccgta ttttcgtatt
     1801 ttcaacccga caacccaggg cgagaaattt gatcgcgaag gtgagtttat ccgccgatgg
     1861 ttaccggagc tgcgcgatgt accagggaaa gcggtgcatg agccgtggaa gtgggcgcag
     1921 aaagcaggtg tgatgctgga ttatccgcaa ccgatagttg atcacaaaga ggcaaggctg
     1981 cgaacgctgg cagcgtacga agaagcgagg aaaggagcct gataaaacgc agcgagcgtt
     2041 ttatcaggta tggtgctggc ctattaagat tccagcgcca gcgcgcggtt tctgaatttc
     2101 agcgcctgat acagccaaat catctgtacc agtcctacac acgccagtgc gccccaggta
     2161 atttgatcaa atacttcaat atatgcattg atggagtagt tgatcgcccc ggaagcatca
     2221 aacgaagcct gcgatgtctg atcggcaatc acgcccgcca gatagttcgc gatcgcgcca
     2281 gaaagcagca tgtagatgcc ggttaatacg ccggtcacac cggggatttc aatgcgcgta
     2341 atttgcgaca tggcaaccgg gtcgataaac agttccgcaa agcccatcac cgccaggcct
     2401 aataccatca gtggcagaga agagtgacca tacattgcgg accagcgggc gcttaaggtc
     2461 agaatgcaga atccggcgct catcaggcca aggccaagag caaatttccc ccagatgcgc
     2521 acggtacgat tacccgcgac actctctttt accacccacg ccaggaacac accgcacagc
     2581 ataactgcga aggcattaat cgactggaac atcgcggtcg gaacggtata
//
LOCUS       DAJOWB010000282         2629 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJOWB010000282 DAJOWB010000000
VERSION     DAJOWB010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.284"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..136)
                     /locus_tag="OHO85_004927"
     CDS             complement(<1..136)
                     /locus_tag="OHO85_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HCQ2674143.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     V"
     gene            380..637
                     /locus_tag="OHO85_004928"
     CDS             380..637
                     /locus_tag="OHO85_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HCQ2674144.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(687..1637)
                     /gene="uspE"
                     /locus_tag="OHO85_004929"
     CDS             complement(687..1637)
                     /gene="uspE"
                     /locus_tag="OHO85_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCQ2674145.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1789..2541)
                     /gene="fnr"
                     /locus_tag="OHO85_004930"
     CDS             complement(1789..2541)
                     /gene="fnr"
                     /locus_tag="OHO85_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCQ2674146.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
BASE COUNT          673 a          618 c          630 g          708 t
ORIGIN      
        1 gcaccgcatg gaataaaaaa ccttttcttt tacgatttcc gcggcgaaac caaaagctca
       61 tcagaatcaa tgctgcgcag ctaccgaaaa taatgaccag attaagcgca ttatttgtaa
      121 acagttcagc gatcattgtt ttatcaggct cctccagata attgtcgtca tgccggaaac
      181 ccctggcggg gctattttac cgcgacaatt cattcagatc atcaatagtc agggaaggaa
      241 gtagcaacat tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt
      301 ttcagagcag acaacggtga aatgtcatgg tattgttacg tttaggtaac aagaaatttg
      361 tctgcacaag gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt
      421 tctctttggt ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgactat
      481 cggcggcgtt atagaacagt acaatattcc gctgtctgag tggacgacat caatgtatgt
      541 gattcagtca tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc
      601 gttgggaatt tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc
      661 gggccttgtc agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt
      721 gtctgatact ggtcaggttt aatgaccaac agatcgcagc gaagatgatc aatcacctgt
      781 tccgccgtgt tgccgaggaa tgctgctgaa ataccggtgc gtcctaccgt gcccagaacc
      841 acaatccccg cctgtaagtg ctccgccaaa tcaggaatca cctcttctgg cagacctttt
      901 tctacgtgcg tcatgttttc attaatgccg aatttctgcc gcagagcttt cattgccagc
      961 aaatgttgcc cacgaatggc atcgttataa acgctcggat caaattccgg cagttcaatc
     1021 gcgatattaa ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact
     1081 tgttctgcca gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc
     1141 tcttcactgg cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct
     1201 ttcaccatcc acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa
     1261 atcaccgctt ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgcccgccg
     1321 ctgatcactt cctgaatgat ggcttcgaag ggacggttat gccagaccac tttaatttca
     1381 atgggaacgc cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg
     1441 ctgatgacgc cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg
     1501 tatgagaagt catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa
     1561 taaacagctc gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg
     1621 ttctgataca tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa
     1681 aagattaacc catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc
     1741 agaaaaattt aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt
     1801 acgcgtatga ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc
     1861 tttgactgcc agcatgccgc ttttctggaa gcgacccagc agacggctga tggtttctac
     1921 cgtcaggccc agatagttac cgatatcgcc acgagtcatc gtcaggcgga attcacgagg
     1981 ggagaagccg cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc
     2041 ctcggcattt ttcttcgaca acagcaggat catgtcctga tcgcctttga tttcaccgct
     2101 catcagacgc atcatctgct gacgcagatt cggcatttta ccagacaaat cgtccagcgt
     2161 ttcgaacggg atttcacata ccatcgaggt ttccagcgcc tgcgcgaagc tcgggtgatg
     2221 gccgctgccg atggcgtcaa atcccaccag gtcgcctgct aaatggaaac cagtgatttg
     2281 ctcgtcgcct tgctcagtga tggtataact tttaatcgta ccggagcgga tggcataaag
     2341 cgatttaagt tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg
     2401 ctcaatgata ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag
     2461 ctggctgatg ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat
     2521 aattcgcttt tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa
     2581 catcttttta gggagcaagt aagtctaagc aaaccttaac agcagagaa
//
LOCUS       DAJOWB010000283         2628 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJOWB010000283 DAJOWB010000000
VERSION     DAJOWB010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.285"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..238
                     /locus_tag="OHO85_004931"
     CDS             <1..238
                     /locus_tag="OHO85_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HCQ2674147.1"
                     /translation="DKNEEVRIEAIIGLSYRKDKRVLSVLCDELKKNTVYDDIIEAAG
                     ELGDKTLLPVLDTMLYKFDDNEIITSAIDKLKRS"
     gene            266..1224
                     /locus_tag="OHO85_004932"
                     /pseudo
     CDS             266..1224
                     /locus_tag="OHO85_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            1236..1697
                     /locus_tag="OHO85_004933"
     CDS             1236..1697
                     /locus_tag="OHO85_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCQ2674148.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            2404..>2628
                     /locus_tag="OHO85_004934"
     CDS             2404..>2628
                     /locus_tag="OHO85_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674149.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLS"
BASE COUNT          826 a          470 c          604 g          728 t
ORIGIN      
        1 ggataaaaat gaggaagtcc gtattgaagc aataatcgga ctttcctaca gaaaagataa
       61 aagggtttta tctgttttat gcgatgagtt aaaaaaaaat actgtttatg atgatatcat
      121 tgaagctgcg ggtgaattag gtgataaaac gctacttcct gttttagata ctatgttgta
      181 caagtttgat gacaatgaaa ttataacttc cgctattgat aagctgaagc gttcatagcg
      241 tgagttgcct atgcacagtg ggggattccc gccggcacgg tgcagatgct cgaccggctg
      301 gaaagtgaaa tcctggctga ccgggtgagt gaggaaagcc gccgctggct ggcatcgtgc
      361 ggcctgaccg tggagcagat gaaaaaccag atggacccgg tgtacacgcc ggcgcgaaaa
      421 atccacctgt accactgcga ccatcgcggc ctgccgctgg cgctcatcag cacggaaggg
      481 gcaacagcgt ggtgcgcaga atatgatgaa tggggcaacc tgctgagtga tgagaacccg
      541 catcatctgc agcagctcat tcgcctgccg ggtcagcagt atgatgagga gtccggcctg
      601 tattacaacc gccaccgcta ttatgacccg ctgcaggggc ggtatatcac tcaggatccg
      661 attgggctga aggggggatg gaatttttat cagtatccgt tgaatccggt cataaatgta
      721 gatccgcaag gtttggttga tataaattta taccccgaaa gtgatcttat ccattctgta
      781 gctgatgaga ttaatatccc aggcgttttc acaatcgggg ggcatggtac ccccacatct
      841 attgaatccg caacgcgcag tatcatgaca gctaaagatc tagcatatct aattaaattt
      901 gatgggaatt ataaagatgg gatgacagtt tggttatttt cttgtaatac aggtaaagga
      961 caaaattcat ttgctagcca attagctaaa gagttacata caaatgtaat aggacctgac
     1021 acgctatgga cgtggtgggg gcgaggaact aatggtaagt taaaaatgga tacagtgcta
     1081 acagcaccaa cgaaccttaa ttcaaataag gatctaatgg ctataacaac aaaagacctt
     1141 ggtaattgga taacatatgg gccatctggg caccccattt ctaatatgca aggtacgcca
     1201 gaaaaaccca gtgatataag ataggttgta gatgtatgaa agcatgcttg ttactatttt
     1261 tttatttttc tcttattggt caattgcatg gtgctgatgt gaaaataaaa gaaaacgaaa
     1321 gtgtgatggg atctacagca atgacctatg atttaagtga agaaaagctg atgaaactga
     1381 agtataaatc acaacatggt gattcagagg catcatttcg actatatcaa tattactgct
     1441 ttactaaaaa taatattgat aaacaattgc gattcttgga aagatcagca tctcagggga
     1501 atgttacagc gcagtttaat tatggggtct ttttatcaga tacaaatcca acattatcag
     1561 aatattataa tttgaataga gctatttatt ggatggaatt cgctgtaaat aacggtaata
     1621 ttgatgcaaa gagcaaactt caagagctca aaaagctaaa gcgaatggat agaaggaaga
     1681 ataaggagaa tccgtaaaag caatagttca tcagactgcc gcagcagtag tatgatgatg
     1741 aatcagggct gtactataac tattatcggt aacattaacc gcagcagggg cggtataaca
     1801 ctcaggctcc gattgggctg gaaggtaggt ggaattttta tcagtatccg ttgaacccga
     1861 tttctggaat cgatcccctt gggattagcc acctgtttat acagtataac tttaggtatg
     1921 ctttcgtgtg tttcagatat accatgtgat gataattttt atgatgtgct tagtattcca
     1981 gtcgcttctg gtaataatat gcaatgtaaa aataatcccc gttgtacaca tttataaata
     2041 gatggccaat acctcaaggc ctatggagtt ggaatgttaa tgacccggga gcaagcaata
     2101 gaaaacctga cggcatacga ttagtaccgt cagttaatac aggaacttat aatcgcaatg
     2161 gattttcaat acatagttgt ttaaacgctt ttggtccgtc tttaggacct cgattctgtt
     2221 ccgagggttg tataactggt ttatctaatg acatgcaaaa attaaatgag ttaatatttt
     2281 cagagccaga tagcacatta acagttacgg actaaaaaat atggcaaggt tattaacaat
     2341 tgtatttttt tctctatttc tgcgagtgca gttactgcaa gacaatttca gagagagtta
     2401 gatttggcag acaaagcaat attatgggca ctaatatcag ccagtactaa agagggacga
     2461 aaagcgtgtt cactaagcta ttttgcctgt aaggccgcag aggctgaact cggactggca
     2521 tatatggctg cgaatgataa caaggaattt ctcacatcct tatctaatat aatgaggtac
     2581 aaaatagatg ctggactttc cgaatcctat acatgctatt tattaagt
//
LOCUS       DAJOWB010000284         2565 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJOWB010000284 DAJOWB010000000
VERSION     DAJOWB010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.286"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(152..301)
                     /locus_tag="OHO85_004935"
                     /pseudo
     CDS             complement(152..301)
                     /locus_tag="OHO85_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(479..1672)
                     /locus_tag="OHO85_004936"
     CDS             complement(479..1672)
                     /locus_tag="OHO85_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCQ2674150.1"
                     /translation="METTSVIILNGFLGAGKTTLLKNLLIQAHEKQLSVSVIVNDMSE
                     LDVDGVLIANTDIVDAKDNNFVSITADSISSPAGLKKMDLAINHLLDHNKPDVMLIET
                     SGSSHPLPLVKYLRRHSRVRLKAFLTLVDTVMLHEDYNDGKKLIPTLQENLRHNKRGL
                     ENLLAEQIMFCNRLLLTRNDRLPFDIISAVAKAIHPLNPSVDVLAVSWGNIELSTLLA
                     IPDYNFDRVELLISELEALVGNMDTPCNNEELIWRVIRDDRPFHPQRLWDTCHRFMGM
                     GVYRSKGFFWLPGRDDLALLWNQSAGSISLALIGYWKAGVLEHTDNNLTREERSALQR
                     HIDTASGRFGDRCCQLTIIGNATEVNDFTHALSLCLLTEEEIQWWMSGGVFPDPWPQK
                     VIRLS"
     gene            complement(2127..>2565)
                     /locus_tag="OHO85_004937"
     CDS             complement(2127..>2565)
                     /locus_tag="OHO85_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2674151.1"
                     /translation="ALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNG
                     SCYRANETRQFARMLGLEPKNTAVRSTESNGIAESFVKTIKRDYISIMPKPDGLTAAK
                     NLAEAFEHYNEWHPHSALGYRSPREYLRQRACNGLSDNRCLEI"
BASE COUNT          608 a          633 c          597 g          727 t
ORIGIN      
        1 ttaacttact tcatgaagct tactgcttct atttccactt ctttttctta ttaatcgctc
       61 ccctaatcca cgcattcatc tgctctacag gctctaaaat attttttctg agtactgagc
      121 acaaactgct ccataaaaaa cagaccacaa gtttgggcat gatactgatg tagtcacgtt
      181 ttattgtttt catgaagctc tctgctattc cattactcac cggactccgc accgccgtgt
      241 tcttcgattt aagtcccaac atccgagcga actggcgtgt ttcattaacc cggtagtcag
      301 agtcagcggt cgaaagacct cccaggtagc tactctgcat acccactata atgcaactat
      361 tctcgtgacg tgtgtttcag ctctcccccg gctatgatac gagatggtat ggagagccct
      421 tctggggcca ctgcctgcac cagatgcaac aggaagcctg atatgaatgc aatttctgtc
      481 aggacaggcg gataactttc tgtggccatg gatcagggaa tactccaccc gacatccacc
      541 actgaatttc ttcttcagtc aacaagcaaa gggataaggc atgtgtaaaa tcattaactt
      601 cagtggcatt gccaatgata gtcaactgac agcagcgatc gccaaatcgc ccagaggctg
      661 tgtcgatgtg tcgctgtagc gctgaacgtt cttcacgagt taaattattg tcagtatgtt
      721 ccagtactcc agctttccag tagccaatga gcgccagact gatgcttcct gctgactgat
      781 tccagagcag ggcaagatca tctctcccgg ggagccaaaa aaaaccttta ctgcgataaa
      841 cgcccatgcc cataaagcgg tgacaggtat cccataatct ttgcggatgg aatggcctgt
      901 cgtccctgat gacgcgccat ataagttcct cattgttgca cggagtatcc atgtttccta
      961 caagtgcttc cagttcactg atgagtaact caacacggtc aaaattgtag tccgggatcg
     1021 ccagtaatgt gctcagttca atatttcccc atgataccgc gagcacatcc acggaggggt
     1081 taagtgggtg tatggctttt gccacagcgg agataatgtc aaagggcagg cggtcatttc
     1141 tggtcagcag cagcctgttg cagaacatga tttgctcggc cagcaaattc tccaggccgc
     1201 gtttgttatg ccggagattt tcctgaagag tggggatcag ttttttccca tcattgtaat
     1261 cttcatggag catgacagta tcaacgagtg ttaaaaacgc cttcaggcgt acacgtgaat
     1321 ggcgacgcag gtatttgacc agaggaagcg gatgactgct tccggaggtt tcaatcagca
     1381 tcacatccgg cttgttgtga tcaagcaggt ggttaattgc gagatccatc tttttgaggc
     1441 ctgcggggct gctgatgctg tctgcggtga tacttacgaa attattgtct tttgcatcta
     1501 caatatctgt gttggcgatc agcacgccgt ccacatccag ttcgctcatg tcattgacta
     1561 tgacagagac tgaaagctgt ttttcgtgtg cctgtatgag caggtttttc agcagagtgg
     1621 ttttccctgc gccgaggaag ccattgagaa tgatgacaga tgttgtttcc atatcagcag
     1681 tacaccagtt agttagcgag gaaggcgctt ggtggtacaa agagcatccg gattgaatcg
     1741 cataacgtcg acgggagttg ttgtcgtttt tcctgtgagg tctcaacctg ccacagaacg
     1801 caccggattt attggtattt agtctccatc agcataatta tcaccatttt tatctccggg
     1861 ggaagatttg ttctgaacag gagtgggagg tgtgacctcc tgaaagtaat tacagattgc
     1921 tagttgttgt atttttattc gaatatgtct cttcgtatca gtgatagcac ctagataaag
     1981 tatattccgg gagcgttatc tgttctagat atcagagagt cagtcatttg taaaaaagag
     2041 agctgcatca ttctattttt cctgtctatt caccctgttg tcgtatctaa aagacatccg
     2101 ttccgaggat gtcggtggat ttgcccctat atttccagac atctgttatc acttaaccca
     2161 ttacaagccc gctgccgcag atattcccgt ggcgagcgat aacccagcgc actatgcgga
     2221 tgccattcgt tataatgctc gaacgcctct gcaaggttct ttgctgccgt taacccgtct
     2281 ggtttgggca tgatactgat gtagtcacgc tttatcgttt tcacgaagct ctctgcgatt
     2341 ccgttactct ccgtactccg caccgccgtg ttcttcggtt caagtcccaa catccgggcg
     2401 aactggcgtg tttcattagc ccggtagcat gaaccattat ccgtcagcca ctccactgga
     2461 gacgacggaa gatcgttgcc gaagcggcgt tccaccgctc ccagcatgac gtcctgtact
     2521 gtttcactgt tgaagccgcc ggtagtgacc gcccagtgca gtgcc
//
LOCUS       DAJOWB010000285         2510 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJOWB010000285 DAJOWB010000000
VERSION     DAJOWB010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.287"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..313
                     /locus_tag="OHO85_004938"
     CDS             <1..313
                     /locus_tag="OHO85_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000611663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674152.1"
                     /translation="DGKSVSYHVPLCGVFICDETLRDGIIINGEFEKAKFSLYDSIEP
                     IICDRWPQAKIYRLADIENVKKQIAITREEKKVKSAASVTRSRKTKKGQPVNDNPESA
                     Q"
     gene            complement(346..1464)
                     /locus_tag="OHO85_004939"
     CDS             complement(346..1464)
                     /locus_tag="OHO85_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCQ2674153.1"
                     /translation="MRTSLVTREEMIEAIEQHTSCISTRDIPGVIANYFMITKQLYRR
                     KDKNAVHRILLSDIREYLLEQGHLNYATVAAETRKEAHRMKATNVKSEKTHAPSVQES
                     ELVVVQNQSDEIPVLEWQGARVVTTETLARGYGTETIRIRQNHHENKVRFVEGKHFFK
                     VEGESLRELKHRVALNYSVKIARNVRSLTLWTERGAARHAKMLETDQAWAFFEKLEDS
                     YFRQKEQQPIAIPQTLPEALRLAAELAEQKQLLEQKAHQLNQQLVAAAPKVDFADRVS
                     VAKGILIGNFAKVVGLKQNALFAWLRENGILIASGGRKNVPFQQYINAGYFTVKEVVL
                     DDEDGYQIRLTPQLTGKGQQWLTRKLLDAGLLKPVAAE"
     gene            complement(1461..2024)
                     /locus_tag="OHO85_004940"
     CDS             complement(1461..2024)
                     /locus_tag="OHO85_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCQ2674154.1"
                     /translation="MNICSNELCFLTTDVTLNLTSCALRHFRFMLIRHLIKTGAGIGL
                     PLKSKAIQTPLASFFVSAYTHTSTMVGCMGLPSGRLVTLDASKANSVQSTASKIGLFC
                     GGYIPTSKEAANMATIPTPTHPEFIWRFYSCQKRHYHFVIAPTEDEARSQLPDAPCIF
                     SARFSTDSRNSLSYWCLPVNASAQEGL"
     gene            complement(2021..>2510)
                     /locus_tag="OHO85_004941"
     CDS             complement(2021..>2510)
                     /locus_tag="OHO85_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCQ2674155.1"
                     /translation="TQLAAKIFAECNPERFPNITIAIDGDGLGKATADLMYEYYGITV
                     QRIRWGKKMHSREDKSLYFDKRAYANVQAAEAVKSGRMRLDKGNETIEEASKIPVGIN
                     SAGQWKVMSKEDMKKKLNLHSPDHWDTYCFAMLADYVPQDEVLSVEDEAQVDEALAWL
                     NE"
BASE COUNT          632 a          618 c          591 g          669 t
ORIGIN      
        1 tgatggtaaa tcagttagtt accatgtgcc actgtgtggc gtgtttatct gcgatgaaac
       61 tctccgtgat ggaattatca tcaacggtga attcgaaaaa gcaaaattta gcctttatga
      121 ctccatagaa ccgatcatct gcgaccgctg gccgcaagca aaaatatatc gcctggcaga
      181 tattgaaaat gtaaaaaaac aaattgccat cactcgcgaa gagaaaaagg ttaagtcagc
      241 cgcatcagtt acgcgcagcc gtaaaaccaa gaaggggcag ccagtaaacg acaaccccga
      301 aagcgcgcaa taaattatgc ccggcatcaa ccgggcattc ttccattatt cagccgccac
      361 cggttttaac aagccagcat cgagcagttt acgcgtcaac cactgctggc ctttacccgt
      421 taattgaggc gtcaaccgta tctggtagcc atcttcatca tccagcacca cttctttcac
      481 cgtgaaatac cccgcgttga tgtactgctg gaacggcaca tttttacgtc caccggacgc
      541 tatcaggatg ccgttctccc gtaaccaggc aaacagcgcg ttttgcttaa gtccaacaac
      601 ctttgcaaaa ttcccaatca ggatcccttt agctactgat acccggtcgg caaaatcgac
      661 tttaggagcg gcggccacca gctgctgatt tagctggtgg gctttctgtt ccagaagctg
      721 cttttgttca gccagttcgg cagccaggcg tagggcttca ggaagcgtct gggggattgc
      781 gatcggttgc tgttcttttt gtcggaagta gctgtcttcc agtttttcaa agaatgccca
      841 tgcctgatcg gtttcgagca ttttagcgtg gcgggctgcg ccgcgttctg tccagagggt
      901 gagtgagcga acattgcgag caatttttac agagtagttt aaagctactc tgtgcttcaa
      961 ctcgcgcaat gattctcctt caactttgaa aaagtgcttc ccttcaacaa agcgtacttt
     1021 gttctcatga tgattttggc gaatacggat tgtttctgtc ccataccctc tagcaagagt
     1081 ctcggttgtc actacacgcg ctccctgcca ttccagaacg ggaatttcat cagactgatt
     1141 ctgaacaacc accagctccg attcctgaac tgaaggtgca tgagtttttt ctgatttaac
     1201 gttagttgct ttcattctgt gtgcctcctt gcgtgtttcg gctgcgacgg ttgcgtaatt
     1261 cagatgcccc tgttcgagca ggtattcgcg gatatcagac aacagaatgc ggtgaacagc
     1321 gttcttgtcc tttctccggt aaagttgttt ggtgatcatg aagtagttgg caataacgcc
     1381 cggtatatcc ctggtactga tacaggatgt gtgctgttca attgcctcaa tcatttcttc
     1441 acgggtgact aatgacgttc tcatagtccc tcctgagcag aagcgttaac agggaggcac
     1501 cagtaactga gagaattgcg tgaatcagtg gaaaaacggg cagagaaaat acatggggcg
     1561 tcaggaagct gagagcgggc ctcatcttct gttggtgcaa taacgaagtg atagtgacgt
     1621 ttttggcagg agtaaaagcg ccagataaat tcaggatgag ttggggtagg gatagtagcc
     1681 atattggcag cctccttaga cgttggtatg taaccaccgc agaagagacc aatcttgctg
     1741 gcggtggact gtacggagtt ggccttactg gcgtccaagg taaccagcct acccgaaggt
     1801 agccccatac agcccaccat tgtagaggtg tgcgtgtacg ccgatacaaa aaaagacgcg
     1861 agcggcgtct gtatcgcctt agacttaagc gggaggccaa tcccggcacc cgttttaatg
     1921 aggtgcctga taagcataaa ccgaaaatgc ctcaaggcgc aagaggtcag gttcaatgta
     1981 acatcggtag ttaaaaaaca caattcatta gagcaaatgt tcattcatta agccatgcca
     2041 gagcttcatc aacctgcgct tcgtcttcga cgctaagcac ttcatcctgg ggaacataat
     2101 ccgccagcat agcgaaacaa tatgtatccc aatggtctgg tgagtgcagg ttgagttttt
     2161 tcttcatatc ctccttactc atcaccttcc attgacctgc ggagttaatc cctacaggga
     2221 ttttcgacgc ttcctcaata gtttcattac ccttatccag tctcatacga ccagatttta
     2281 cggcctctgc ggcttgaaca ttggcataag cacgtttatc aaagtacagg ctcttatctt
     2341 cacggctatg catcttttta ccccagcgta tacgctgtac ggtaatacca taatactcgt
     2401 acatcagatc cgctgttgct ttacccagcc catcgccgtc tatcgctatg gtgatatttg
     2461 ggaatcgctc aggattacat tctgcgaaaa ttttggcggc aagctgcgtt
//
LOCUS       DAJOWB010000286         2438 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJOWB010000286 DAJOWB010000000
VERSION     DAJOWB010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.289"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            267..1817
                     /locus_tag="OHO85_004942"
                     /pseudo
     CDS             267..1817
                     /locus_tag="OHO85_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162635.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            1817..2392
                     /locus_tag="OHO85_004943"
     CDS             1817..2392
                     /locus_tag="OHO85_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCQ2674156.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTY
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNNLMQVASEHIAPLQDAADLEIATEEE
                     ISLLEAWKKYRVLLNRVDTSTAQDIEWPALP"
BASE COUNT          675 a          589 c          653 g          521 t
ORIGIN      
        1 cgtcggaaac cagcgcagaa tcctcaaaaa cggctgccgc atcgtccgcc agttcggcgg
       61 cgtcatcggc atcatctgcg tctgcttcaa aagatgaggc gaccagacag gcgtcagcag
      121 caaagggcag cgccacgacg gcatccacga aggcgacaga ggcagctggc agtgcgacgg
      181 cggcagctca gagcaaaagt acggcggaat ccgcggcaac gcgcgccgag acagcggcaa
      241 aacgggcaga ggatattgca tccgccgtgg cgcttgagga tgcgagcacg acgaaaaagg
      301 ggatagtaca gctcagcagt gcgactaaca gcacttccga gtcactggcg gcaacgccaa
      361 aagccgttaa ggccgcgtat gagctggcta acgggaaata caccgcacag gatgcaacga
      421 cagcacagaa agggatagtt cagctcagca gcgcgaccaa cagcacatct gaaatgctgg
      481 cggcaacgcc aaagtcggta aaggcagcct atgaccttgc taacgggaaa tatactgctc
      541 aggacgctac gacagcacaa aaaggaattg tccagctcag tagtgcaacc aacagcgcat
      601 ctgaaacgct tgccgcgaca ccgaaagcag tgaaagcagc taatgataat gcgaatggtc
      661 gggtaccttc tgcccgaaag gtgaatggta aggcgctttc agcggatata acactgacgc
      721 cgaaagatat tggtacgctt aactcaacaa caatgtcatt cagcggtggt gctggttggt
      781 tcaaattagc aacggtaacc atgccacagg cgagttctgt tgtttaaatt acgttgattg
      841 gtggcgcggg atttaacgtg gggtcacctc aacaggcagg tatatctgaa cttgttttgc
      901 gtgcaggtaa tggtaatccg aaggggatta ctggtgcttt atggcagcgc acatcgacag
      961 ggtttacaaa ttttgcctgg gtcaatacat ctggtgatac ttacgatatt tacgttgcaa
     1021 tcggaaatta tgcgactggt gtaaatattc aatgggatta taccagtaat gccagcgtga
     1081 ctattcatac gtcaccagca tattctgcta ataagccgga agggttaacg gacggtacag
     1141 tttattcact ctatacgcca tcagagcagt tttatccgcc tggcgcacca atcccgtggc
     1201 catcagatac cgttccgtct ggttatgccc tgatgcaggg gcagactttt gacaaatccg
     1261 cttacccgaa acttgcaacc gcttatccgt caggcgtgat ccctgatatg cgtggctgga
     1321 cgattaaggg caaacctgcc agtggtcggg ccgtattgtc tcaggaacag gacggcatta
     1381 aatcgcacac ccacagcgcc agtgcatcca gtacggattt ggggacgaaa accacatcgt
     1441 cgtttgatta cggcactaaa tccacgaata acaccggggc gcatacgcac agtgtgagcg
     1501 gtacagccgc aagtgccgga aaccatactc atagtgtcac aggcgcatca gcagtcagcc
     1561 agtggtcaca aaatgggtca gtacataagg tagtgtctgc ggccagtgtg aatacaagtg
     1621 ctgcaggagc gcacactcat agtgtcagcg gcacagctgc atctgcaggt gctcacgcac
     1681 atactgtcgg tattggtgct catacgcact ctgttgcgat tggctcacat ggacacacca
     1741 tcaccgttaa cgctgctggt aacgcggaaa acactgtcaa aaacatcgca tttaactaca
     1801 ttgtgaggct tgcataatgg cattcagaat gagtgaacaa ccacggacca taaaaattta
     1861 taatctgctg gccggaacta atgaatttat tggtgaaggt gacgcatata ttccgcctca
     1921 tacaggtctg ccagcaaaca gtacctatat tgcaccgcca gatattccgg ctggcttcgt
     1981 ggctgttttc aacagtgatg agtcatcgtg gcatctcgtt gaagatcatc ggggtaaaac
     2041 cgtctatgac gtggcttccg gcgacgcgtt atttatttct gaacttggcc cattaccgga
     2101 aaatgtcacc tggttatccc cggaagggga gtttcagaag tggaacggca cagcctgggt
     2161 gaaggatacg gaagcagaaa aactgttccg gatccgggag gcggaagaaa caaaaaacaa
     2221 cctgatgcag gtagccagtg agcatattgc gccgcttcag gatgctgcag atctggaaat
     2281 tgcaacggag gaagaaatct cgttgctgga agcatggaaa aagtatcggg tattgctgaa
     2341 ccgtgttgat acgtcaactg cacaggatat tgaatggcca gcactgccgt agggtaaaac
     2401 atataaattc tataattaga tgtatctttc catttacg
//
LOCUS       DAJOWB010000287         2437 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJOWB010000287 DAJOWB010000000
VERSION     DAJOWB010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.290"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..180)
                     /locus_tag="OHO85_004944"
     CDS             complement(<1..180)
                     /locus_tag="OHO85_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="internal head protein"
                     /protein_id="HCQ2674157.1"
                     /translation="MEQFNINKGVTIKPGLDVLPPPVTDDEYRALMAGEDRYLMTESN
                     TLEEIEATFFYDTPIH"
     gene            complement(182..784)
                     /locus_tag="OHO85_004945"
     CDS             complement(182..784)
                     /locus_tag="OHO85_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="odaE"
                     /protein_id="HCQ2674158.1"
                     /translation="MTKNKYATVDFDQVNEKGLKSLIAAINKTGVTVIEVDSSNRATT
                     KDGVKVKTAKLVLNDGQILAIQVNDTGDISSVKLNGKAIPNAQSPDIKTLGTVMGQAA
                     RKNSAKFQKSLIAKAKRVANPVDKKPAVKSNFQRLQEAKQRNAQVVAAYKSAQNSVSF
                     NQQQITDLRAKLDKETGRLNNEKARNGELKRRLKQLKAGN"
     gene            complement(771..1214)
                     /locus_tag="OHO85_004946"
     CDS             complement(771..1214)
                     /locus_tag="OHO85_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCQ2674159.1"
                     /translation="MIGWGVCVLALALADRYLLKRKDITHLELGDVEVKQGFIRVPFK
                     YRSKFPFLRGATVRYWIRDVQKPTTVIEGEQRCLTSAEQGENSEWLYIPTEYMGKGER
                     LWHFNVMVTHGDSFINPLYRIFPVTQQIRRSYVINLAQDVSDDEK"
     gene            complement(1211..1540)
                     /locus_tag="OHO85_004947"
     CDS             complement(1211..1540)
                     /locus_tag="OHO85_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCQ2674160.1"
                     /translation="MLDTQELAPVAIALLLSVIGGIGTFLMDVRDGRQSGNLLGLVTE
                     IFVAVTAGAVAYLLGQHEGWELSITYLMVTIASNNGHEVISGMKRVNIDSILNVLTSL
                     VKKGGGK"
BASE COUNT          594 a          633 c          617 g          593 t
ORIGIN      
        1 gtggatcggc gtgtcataga agaacgtagc ctcgatttcc tccagggtgt tggattccgt
       61 catcagatag cggtcctcac cggccattaa tgcgcgatat tcatcatcag tcactggcgg
      121 gggaagcacg tcaagcccag gcttgatcgt caccccttta ttgatattga actgttccat
      181 gttaatttcc tgctttcagt tgcttaagac ggcgtttgag ttcgccattt cgggcctttt
      241 cgttattgag tcggcctgtc tccttatcca gcttcgcccg caaatcagtg atctgctgtt
      301 gattgaaaga caccgaattc tgcgcggact tataagcggc aaccacctga gcattccgct
      361 gttttgcttc ttgcaggcgc tgaaagttgg atttaactgc cggtttcttg tctaccggat
      421 tggcaacacg cttcgctttg gcgatcagtg atttctggaa ttttgcggag tttttgcggg
      481 ccgcctgtcc cataacggta ccaagcgtct tgatatccgg cgactgagcg ttaggaatag
      541 cttttccatt cagtttcaca gacgatatat cgccagtatc gtttacctgt atggcaagaa
      601 tttgtccgtc gttaagaacc agctttgcgg ttttaacttt aacgccatct ttcgttgttg
      661 cgcggttgct ggagtcaacc tcaattaccg taaccccggt tttattgatc gccgcgataa
      721 gggatttcag ccccttttca ttaacctggt caaaatcgac cgttgcatac ttatttttcg
      781 tcatctgaca catcctgtgc gagatttatt acgtaacttc tgcggatttg ctgagtaaca
      841 gggaaaatcc gatacaacgg gttaatgaac gagtcaccat gcgtaaccat gacgttgaaa
      901 tgccacagcc gttctccttt acccatatat tcagtgggta tgtacaacca ttcactgttt
      961 tcgccctgtt cagccgacgt cagacaacgt tgttcgcctt caatcactgt cgttggcttc
     1021 tgaacatcgc ggatccaata tctgaccgtt gcgccgcgca aaaacgggaa tttagaccgg
     1081 tatttgaacg gcacccggat gaagccctgt ttaacttcca catcaccaag ttctaaatgc
     1141 gtgatgtcct tgcgttttag caaatagcga tcggctaagg ctaacgcaag aacgcataca
     1201 ccccagccaa tcatttccca cctccctttt tcaccaaact tgtaagaaca ttcagaatgc
     1261 tatcgatatt cactcgtttc atccctgaaa tcacctcatg accgttattg ctggctatcg
     1321 ttaccattaa gtacgtaatt gataactccc agccctcgtg ttgccccaat aggtacgcca
     1381 ccgcgccagc tgtcactgca acaaagatct ccgtaaccaa tcccaacaaa ttgccagact
     1441 ggcgaccatc tcggacatcc atcaggaacg tgcctatccc accaattact gaaagcagga
     1501 gcgcaatagc aactggagct aattcctgtg tgtcaagcac aagttccctc ctacgttgtc
     1561 aggaggtaat ggtatgcaaa gtaacttctc aaccggtcat gttgcttgac atcctccacg
     1621 tcctttagag cgtggaggat gtgtgcgccc agcatgggcg cgatcttgga ggtgaaagcc
     1681 ctcccgcgag ctgaccacag cgagcgaaat gaagtgcaac tgcgtgaggg cgaccgggcg
     1741 tggggaggaa gcacggaatg aacccgtgag ccgatgaacg agaatcggat atgaggcgcg
     1801 atggagcaag gcgagcaggc acatcactgc gaagctttcg tggtcaagtc gaagtcgcgc
     1861 agatccgacg gttgtgcggg gaaggatcgc gttcttacct ggggagttct cgcctcatgc
     1921 ctgaaagggc aacgtcgtaa gacggagcga gaagtcagca gaggccgtag tagtcaggac
     1981 tccgacgaag ggccgaacga gaaggaaagc cagaagatat gactcttgac aggacattgc
     2041 gtcagaagcc gaggaagccc ggtacggacc cgcatgccgg gtggtgtggc aggggcgcag
     2101 cccttgtgct cccccctatg ccgatcaagc agaacattgt gctgggcctc tgacgcagct
     2161 ccttgatggt cttggcatcg tgcttaagtg gattggtgtc gtccctgata ggatcatgga
     2221 ggcagaatga aactcaaaaa tccgggatag cacgcaaaac aggctgcaca aaaagcgcag
     2281 gttgcgtagg agtgaaatcc cgctcccaaa aaatggtgca gaagttgcgg cagtggtcat
     2341 ccgacgcact ggaaaataac aaacccgccg tgaccagaat cggaggcctg agggagtgac
     2401 cgaaaaaccg gacaacccag gcagcaaata cccgctg
//
LOCUS       DAJOWB010000288         2404 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJOWB010000288 DAJOWB010000000
VERSION     DAJOWB010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.291"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            19..831
                     /locus_tag="OHO85_004948"
     CDS             19..831
                     /locus_tag="OHO85_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCQ2674161.1"
                     /translation="MIGTLVNISKIIIQKRELIWQMSKRDVMGRYKGSFLGLAWSLFN
                     PILMLTVYTFVFSVIFKSRWGSSPDAGHADFAIILFTGLIIFNLFAECINRSTSLITS
                     NVNFVKKVVFPIEILPIVNLLAALFHASISLIVLFIAILVFKHQLHFTVLLLPVITIP
                     LMLSILGLSWILASLGVFVRDIPQTISIIVTILMFLSPVFYPLSALPVVFQKIVMLNP
                     LAFMIEEARKVVYWGNEPNWTMLAINMLIGLVICAAGFLFFQKTKKGFADVI"
     gene            821..2230
                     /locus_tag="OHO85_004949"
     CDS             821..2230
                     /locus_tag="OHO85_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCQ2674162.1"
                     /translation="MSSDNIAISVSNISKCYNIYQNPRDRLKQFFLPKVQSMVGKPVQ
                     RYYNEFWALNNINFTINKGESAGIIGRNGSGKSTLLQLITGTLTPTTGTIQTKGRIAA
                     LLELGSGFNPEFTGRENIYLNGAVLGLSRAEIDAKFDAIAGFADIGTHLDQPVKTYSS
                     GMMVRLAFAVQVQLEPDILIVDEALAVGDALFQKRCFQQIEKLTSNGVTLLFVSHDQE
                     SVRTLTNRAILLDRGTQLSTGLSSEVLLEYRRLLHREESKYFSQLAKEVAAHADATRD
                     INQTPTAPAKIEISANAETPAVEETSPSRTDKLSFGEGEAEVENVELFTASGERSNAF
                     NPGDKIRVRVNCKVNKDINHINVGIRIRNKEGVKIYSWGTLNQDMYVRFHQLKDPQFW
                     EREFKAGERFYVDMEFECTLGTNLYEIQAAISYEGTPNYFSQRILHWRDEAAFFHVNV
                     LRDEYFFGGILDLKMTAEW"
BASE COUNT          714 a          466 c          494 g          730 t
ORIGIN      
        1 aacaaagcgg taaaaataat gattggaact ttagtaaata tttcgaagat aattatccag
       61 aagcgcgagt taatctggca aatgtctaaa agagacgtta tgggacgcta taaaggatcc
      121 tttcttggtc tggcatggtc acttttcaac cctatattga tgttaacagt ctacacattt
      181 gtattctcgg ttatattcaa atctcgttgg ggaagcagtc cagatgccgg gcatgcagat
      241 tttgcaatca ttttgtttac cggactgatt atttttaact tatttgcaga atgtattaac
      301 aggtcaacca gccttataac ctcaaatgta aacttcgtca aaaaagttgt tttccctatt
      361 gagatattac ctatagtaaa tctgctggct gctttatttc atgccagcat tagcctcata
      421 gtcttattta ttgcaatact ggtgtttaag catcagcttc attttactgt cctgttactg
      481 ccagtcatta ctattccact tatgctctct atacttggat taagctggat cctggcctca
      541 ctgggcgttt ttgttcgtga tataccacaa actattagta tcatcgtaac aatattaatg
      601 tttttatctc ccgtatttta tccgttatca gcattaccgg ttgttttcca aaaaattgta
      661 atgttgaatc ctctggcatt tatgattgaa gaagcaagaa aagttgttta ctggggaaat
      721 gagcctaact ggaccatgct cgcaattaat atgcttattg gtttagttat ctgtgctgca
      781 ggttttttat ttttccaaaa aacaaaaaaa ggatttgccg atgtcatctg ataacattgc
      841 aattagcgta agtaacatta gcaaatgcta taacatttac caaaacccca gagataggtt
      901 aaaacagttt ttcttaccta aagtccagag tatggttggg aaacctgtac aacgctacta
      961 taacgaattt tgggcgttaa ataacatcaa ctttaccatc aataaaggtg agtcagcggg
     1021 aattattgga cgcaatggtt ctggcaaaag tacattacta cagctaatca ccggcacgct
     1081 aactccaaca acgggcacga tacaaaccaa gggaagaata gctgccctcc tggaattagg
     1141 ctccggtttc aacccagagt ttaccggtag ggaaaacatc tatcttaacg gcgccgttct
     1201 gggattatcg cgggcagaaa tagatgcaaa atttgatgct attgccggtt ttgctgatat
     1261 aggaacccat ctcgaccagc cggtaaaaac atactccagc ggaatgatgg tgcgccttgc
     1321 atttgccgtt caggtacaat tagagccaga tattctcatc gtagatgaag cacttgctgt
     1381 aggcgacgca ctttttcaaa agcgctgttt ccaacaaatt gagaaattaa ccagcaatgg
     1441 cgtaacttta ctgtttgttt cacacgatca agagtccgtg cgtaccttaa caaacagagc
     1501 tattcttctc gatcgtggga cgcaattaag tactggctta tcatcagaag ttttacttga
     1561 atatcgtcgt ttactacacc gggaagaaag taagtatttt tctcagttag caaaggaagt
     1621 tgctgcccac gcggacgcca ctcgcgatat taatcaaacc cccaccgcgc cggctaagat
     1681 agaaattagt gctaatgctg aaacccctgc tgtagaggaa acctcgccct ctcgtacaga
     1741 taaactatcg ttcggtgaag gtgaagcaga ggttgaaaat gttgaattgt ttacggcttc
     1801 tggtgaacgt agcaatgcct ttaaccctgg tgataaaatc agagttcgtg ttaattgtaa
     1861 agtaaataaa gatataaacc acattaacgt tggcattcgt attcgcaata aagaaggcgt
     1921 caaaatttat tcatggggaa cgttaaacca ggacatgtat gttcgtttcc atcaacttaa
     1981 agatccccaa ttttgggaac gtgagtttaa agcaggcgag cgtttttatg ttgatatgga
     2041 atttgaatgt actttaggaa caaacctata tgaaatacag gcggcgatat cctatgaggg
     2101 gacacctaat tatttctcgc agcgcatttt acactggcgt gatgaagccg cgttttttca
     2161 cgttaatgtc ctgcgtgatg aatatttctt tggtggcatt ctggatctca agatgactgc
     2221 agagtggtaa gttgatgaat gatatttcaa taactgatta tctcgggcct ggggtgtatt
     2281 tattacaaaa ttacccaaaa gaaactgagg gcctgattgc agagaagggt tataaggtgc
     2341 acaactgcgc tgatctggcg cagtgtaaag atattcttaa ccgcaataaa gttaatttcc
     2401 tttt
//
LOCUS       DAJOWB010000289         2398 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJOWB010000289 DAJOWB010000000
VERSION     DAJOWB010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.292"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..155
                     /locus_tag="OHO85_004950"
                     /pseudo
     CDS             1..155
                     /locus_tag="OHO85_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            144..431
                     /locus_tag="OHO85_004951"
                     /pseudo
     CDS             144..431
                     /locus_tag="OHO85_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1009..>2398)
                     /locus_tag="OHO85_004952"
     CDS             complement(1009..>2398)
                     /locus_tag="OHO85_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HCQ2674163.1"
                     /translation="KLLLGLSLFLLLEYNACAAKTGGLTLEQRMALLEERLEAAEKRA
                     EKAERMLKSFDIEQHSEIRQISSEQNKKDANSYAVVESTKEKTSSPGFLLSGYNDLKF
                     YGDVEFNIDAASKPGQLVMISSGANSESVNERWDLNGRILLGFDGTRKLDNGYFAGFS
                     AQPLADMHGSVNIDDALFFFGKDDEWKVKVGRFEAYDMFPLNQDTFIEYSGNTANDIY
                     ADGRGYIYMMKEGRGRSDAGGNFLISKQLDNWYFELNTLLEDGTSLYNNGNYHGRYME
                     QQKNVAYLRPVIAWSPTEEFTVSAAMEANVVKNAYGYTDNKGNFVDQSNRSGYGISMT
                     WNGLKTDPENGIVVTLNTAYLDASNEKDFTASINALWKRFELGYIYAHNKIDEFSGVV
                     CNNGCWIDGEGIYNIHTIHASYQFANVMDMENFNIYLGAYYSILDSNCRYSNCDITDD
                     RYGARLRFKYFF"
BASE COUNT          700 a          591 c          478 g          629 t
ORIGIN      
        1 gcccgcatta tgggcgttgg cctcaacacg attttacgtc acttaaaaaa ctcaggccgc
       61 agtcggtaac ctcgcgcata cagccgggca gtgacgtcat cgtctgcgcg gaaatggacg
      121 aacagtgggg ctatgtcggg gctaaatcgc gccagttcgc ccggatgttg ggacttgaac
      181 cgaagaacac ggcggtgcgg agtccggaga gtaacggcat agcagagagc ttcgtgaaaa
      241 cgataaagcg tgattacatc agtatcatgc ccaaaccaga cgggttaatg gcagcaaaga
      301 accttgcaga ggcgttcgag cattataacg aatggcatcc gcatagtgca ctgggttatc
      361 gctcgccacg ggaatatcta cggcagcggg ccagtaatgg gttaagtgat aacaggtgtc
      421 tggaaatata ggggcaaatc cactgcatga caaagtcatc gggcattatc tgaacataaa
      481 acactatcaa taagttggag tcattaccga cgaagcgata gcgataatga ctgaaagcgg
      541 aaaaataaaa acaaattgaa taatgtgttt ggtaatgaat ttatcggggg gaattgggca
      601 tatctaccgc ttacgtcgag ccgctcacac ccggtcaatg acctgacaat ctcccggcaa
      661 gtcccgacgt cgggacttgc gcccctgaca ccgaaaaata gcaggaaccc gctcgctacg
      721 ggtcctcagc tcagtgcaaa cctgactcag ctcctgcatt gcatccccga aattaccaca
      781 ctcagtactt catcacctgc caccaatacc cgtgtcagtc cttcgtgacc tgacgcaatt
      841 acccgactca gagcttcaga gtcagccgcc ggtacctgcc cggtgctcgc ccctgcgcaa
      901 ccaagataaa tcgaccattg gcagactgcg atcattcgta tgcatcgctg atgacgcgcc
      961 cgaaaaacgc gttaatgtaa gtgtggaaca atactttgtt actatgcatc aaaaaaagta
     1021 tttgaaacgt aatcgggcac cgtaacgatc atcggtaatg tcacaattac tatatctaca
     1081 gttactatcc agaattgagt aataagcccc gaggtaaata ttaaagttct ccatatccat
     1141 cacattagca aactgataag atgcatgaat ggtgtgaatg ttgtatattc cttcaccatc
     1201 aatccagcaa ccattattac acacgacacc actaaattca tcaatcttat tatgtgcgta
     1261 gatataaccc agttcgaaac gtttccacag agcattaata ctggcagtga aatctttctc
     1321 attactggca tccaaatagg cggtattaag agtaaccacg atgccatttt ccggatctgt
     1381 tttcagaccg ttccatgtca tgctaatacc ataaccggaa cgattggact gatcgacaaa
     1441 attcccctta ttatcggtat agccataagc attttttacc acgttcgctt ccatcgctgc
     1501 ggagactgtg aattcttccg ttggcgacca ggcaattacc gggcgcagat aagcgacatt
     1561 tttctgctgc tccatatatc gtccatgata attaccgtta ttatataaag atgttccgtc
     1621 ttcaagtaac gtgtttaact caaaatacca gttatcgagt tgtttactga tgaggaaatt
     1681 accaccagcg tcagaacgac cgcgtccctc tttcatcata tagatataac cacggccatc
     1741 agcataaata tcattagctg tattaccgga atactcaatg aaagtatcct gatttagagg
     1801 gaacatatcg taagcttcaa aacgaccgac tttcactttc cactcgtcat cttttccaaa
     1861 aaagaataat gcatcatcaa tattcactga accgtgcata tccgccagtg gttgtgctga
     1921 aaatccagcg aaataaccat tatcaagctt acgggtaccg tcaaaaccta atagaatacg
     1981 gccattaagg tcccaccgtt cattcactga ctcactgttc gccccggaac ttatcattac
     2041 taactgaccg ggtttactgg cagcatctat attaaactcc acatcaccat aaaacttcaa
     2101 atcattgtaa ccagagagca gaaaacctgg agaagatgtt ttttcttttg ttgactcaac
     2161 tactgcatag ctgttagcat cttttttatt ctgctctgaa ctaatttgac gtatttcaga
     2221 atgttgttct atatcaaaac tctttaacat tctttctgct ttttccgccc ttttttctgc
     2281 agcttccagg cgttcttcca gcaatgccat tctttgttca agcgttagcc cccctgtttt
     2341 tgctgcacac gcattgtatt ccagtaataa aaaaagagat aaacccaaaa gtaactta
//
LOCUS       DAJOWB010000290         2324 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJOWB010000290 DAJOWB010000000
VERSION     DAJOWB010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.293"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            86..589
                     /locus_tag="OHO85_004953"
     CDS             86..589
                     /locus_tag="OHO85_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCQ2674164.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEFDGEVVGGGGIAPLAGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(635..1051)
                     /gene="rraB"
                     /locus_tag="OHO85_004954"
     CDS             complement(635..1051)
                     /gene="rraB"
                     /locus_tag="OHO85_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCQ2674165.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1213..2217
                     /gene="argF"
                     /locus_tag="OHO85_004955"
     CDS             1213..2217
                     /gene="argF"
                     /locus_tag="OHO85_004955"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCQ2674166.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT          589 a          549 c          580 g          606 t
ORIGIN      
        1 tattacttta taagtcgctt gcgcacgtaa gaagagagtg gtattaaaca aatgtataat
       61 tactttattt ttattgggtt aaactatgaa taacattgcg ccgcaatcac ctgtaatgcg
      121 tcgcctgacg cagcaggata atcccgctat cgcccgcgtc attcgtcagg tatccgccga
      181 atacggtctt accgctgata aaggctacac cgtcgccgat ccgaaccttg acgagttgta
      241 tcaagtgtat agtcagcctg gccatgcata ttgggtggtt gagttcgatg gtgaagtggt
      301 cggcggcggt gggattgcgc cattagcagg tagtgaatcg gacatttgcg aactgcaaaa
      361 gatgtatttt cttccggcta ttcgcggtaa agggctggcg aaaaaactgg ccttaatggc
      421 gatggagcag gcacgagaga tgggcttcaa acgctgttat ctggaaacga ccgctttttt
      481 aaaggaagcc attgcacttt atgagcattt gggctttgag catatcgact atgcgcttgg
      541 ctgcacgggc catgtcgatt gtgaagtgcg gatgctacgc gaactctaaa aaaatgcccg
      601 ctagtgtgtg ctatcgggca ttttaatcaa ttaattagtg gcgaaccccg tcatcgtctt
      661 cgtcgacaaa atcttcatcg tcgccgtctt cgccgttagg atcttcaaag taagtgcccc
      721 aaccatcgta ctcaacgtca aatttctccg ccagcgtcat cagttgttca acctgggcat
      781 cgatcagatc ggcattcaac gcgcactcgc tgaggatgtc gcagcaaatc acgatatcac
      841 catcttcaac ttccagctct tctggatcgg tcacttcgta accgagttta aacgcttcaa
      901 ctgctgcttt ttccagggtt tccagatcgt ctgcggaaag atggtgttcg atggtgtaca
      961 gcgcgtccgg atcgctgcca tcttccagta attcttcaat aatcaaacgt gtttcttcac
     1021 gctgttcttc cagttgttcc gggtttgcca tggctcattc ctcttaatgt cctgccgata
     1081 ctcttattgt cacacaccac tgacattgcc tccacctttg tgacaaagat ttatgcttta
     1141 gacttgcaaa tgaataatca tccatataaa ttgaatttta attcattgag gcgatagcca
     1201 caggagggat ctatgtccgg gttttatcat aagcatttcc tgaaattact cgatttcacg
     1261 ccagctgaac tcaacagcct gttgcagtta gccgcgaagc tgaaagccga taagaaaagc
     1321 ggtaaagaag aagccaaact cactggtaaa aacatcgcgc tcatcttcga aaaagactcg
     1381 acccgtaccc gatgctcttt cgaagttgcc gcatatgacc agggtgctcg cgttacttat
     1441 ctcggcccaa gcggcagcca gattggtcat aaagagtcga ttaaagacac tgcccgcgtg
     1501 cttggtcgca tgtatgacgg tattcagtat cgcggctatg gtcaggagat tgttgaaaca
     1561 ctggcggaat acgctggcgt gccggtatgg aacggcctga ccaatgagtt tcaccccacg
     1621 cagctgctgg cggatctcct caccatgcag gagcatttac ctggcaaagc gttcaacgaa
     1681 atgacgctgg tctatgcagg tgacgcacgt aacaacatgg gcaattcgat gctcgaagct
     1741 gcggcgctta ccggtctgga tttgcgtctg gtcgcgccac aggcatgctg gccggaagct
     1801 gcgctggtta cggaatgccg cgctctggca cagcaaaatg gcgggaatat tacgctaacg
     1861 gaagatgtag ccaagggagt agaaggtgct gactttatct ataccgatgt gtgggtgtcg
     1921 atgggggaag caaaagagaa atgggcagaa cggattgcat tactgcgtga ttatcaggtg
     1981 aacagcaaga tgatgcagtt gaccggtaac ccggaggtca aattcctcca ctgcctgccc
     2041 gcttttcatg acgaccagac gacgcttggt aagaaaatgg cggaagagtt tggcctacat
     2101 ggcggaatgg aagtgactga tgaggtcttc gaatctgccg ccagcattgt ttttgatcag
     2161 gcggaaaacc gcatgcatac catcaaagcg gtgatggtcg cgacgctcag taaataattg
     2221 atattgctat aaagatgccg ggaaatacaa accatcccgg catcacatta ttttatgcag
     2281 tgataggttt attattccac ccaaattttt ctattgctat acgt
//
LOCUS       DAJOWB010000291         2257 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJOWB010000291 DAJOWB010000000
VERSION     DAJOWB010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.294"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..1541)
                     /locus_tag="OHO85_004956"
     CDS             complement(<1..1541)
                     /locus_tag="OHO85_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCQ2674167.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSNTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAVAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSSAATSAAAAKTSETNAAESQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETSAASSASSAASSATATANSAKAAKT
                     SETNAKSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASASAAGKSAESAASSA
                     STATTKAGEATEQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKSAYDNAEKRLQKDQNGAD
                     IPDKGR"
     gene            complement(1606..2205)
                     /locus_tag="OHO85_004957"
     CDS             complement(1606..2205)
                     /locus_tag="OHO85_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCQ2674168.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVSG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSLRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT          388 a          681 c          615 g          573 t
ORIGIN      
        1 cgtcccttat cgggtatatc agcgccgttc tggtctttct gcagacgttt ctctgcattg
       61 tcataggctg attttactgc ctttggcgtt gccgccagcg tttcagacgt gctgttggtc
      121 gcactgctga gctgtactat cccctttttc gtcgtactcg catcctcaag cgccactgcg
      181 gatgcaatat cctctgcccg ttttgctgct gtctcggcgc gcgttgctgc ggattctgcc
      241 gtacttttgc tctgtgctgc cgccgtcgca ctgccagcag cctctgttgc cttcgtggat
      301 gccgtcgtgg cgctgccctt cgctgctgac gcttgtctgg tcgcctcatc ttttgaagca
      361 gacgccgatg aggccgatga cgccgccgaa ctggcggacg atgcggcagc cgtttttgag
      421 gattctgcgc tggtttccga cgctttcgcg ttcgtctcgg atgtcttcgc tgcggaagca
      481 gacctcgctg ctgcactggc ctgttcagtg gcttcgccag ccttcgttgt ggctgttgaa
      541 gcggacgatg cggcgctttc tgccgatttt ccggcggcgc tagcactggc tgaggcctgc
      601 ccggcacttg ttgacgcggc actggcagac gacgcagccg ctgtttttga gcctgccgca
      661 gctgaggcac tctgtcccgc tgccgtttca gaagacttag cgttcgtctc ggacgttttt
      721 gccgccttcg cagaatttgc tgtcgccgtt gccgaggaag ctgcgctgct ggcgctcgag
      781 gctgcgctcg tttctgatga ttttgccgcc tcttttgaag ctgacgcatc ccgggctgag
      841 gtggcagctt ctgacgcttt cgtggtcgcg gtggatgcag aagtggctgc tgattgttgt
      901 gactctgccg cattcgtttc tgacgttttc gccgcagcgg cactggtggc cgccgagctt
      961 tttgaggact ctgcagcggc agcacttttt gatgcttcag tggcctttgt tgatgccgtt
     1021 cctgcgctgg aagacgctga ctgagccgac gacgcggcct gtccggctga cgtgctggct
     1081 gcacgtgctg aatctgcagc atcagtcgca tgggttgccg cctcacgggc agatgtgctg
     1141 gcatcactgg ctgacttctt cgcgactgcc gtgttctgtg ccaccgcgga cgcgttacgc
     1201 gccacctctt ccaccatcag ttcaaaacgg cgcagagcct ccggacgggc atcatcctcc
     1261 gtcatggcac cgagaaaatc attcagcgta ccgggtcggg aatcttcata cacggtaatg
     1321 gtcccggcat gtgatggcgg gaatccctcc accaacagaa taacgctgta ctgaccgtac
     1381 tcaacgtcca tgctgtaacg cccggcttca tccggatttt ctgaggccag cgtgttcacc
     1441 accaccgtgt tgctgttacg ttttgctttc agctggattg tgcagttctg taccggtttt
     1501 cctgtgccgt ctttcagtac acctgaaatc tttactgcca tattcacccc acaaaaaagc
     1561 ccgcctgaac cggcgggctg tcataacact gtgttacctg gctaatcaga atttataacc
     1621 aacaccaacg atgaacccgt cagtgcgcca gtcgccactg ccggagcctt cataagcgac
     1681 atcaacggcc acggattcgg tcgggttaaa ctgcacgcca gctccccacg ccagagaggt
     1741 gttactgtgg cgaccgtcat cacttccggt cagcacatcg tgcgttttcc ccttgttgtc
     1801 agttacgcgg agataatccc cggagaaagt cgacacacgg ctgtaagcca cacccgccat
     1861 cgcatacgcg ctgaaccatt cattcacgcg tacagacggc cccgccatca cgctgaacca
     1921 gcggttacgc agggaatctt catgccagcg ggtatcgctg taacgggtaa gctggcgatt
     1981 cttgtctcct gcatagctga atgacgtcac cattcccagc gtgtccgtaa actcataacg
     2041 gtatttcacg ttaatcccgt tcagatcatc gctgccggaa acgttcgtcg aggcatgaag
     2101 atagcccgcg ctcagcgtgg actgatgttc agacgcccat gcaggcgcac cggatacggc
     2161 cagacagatg gctgcggaca aaatggcggc ataaagttta cgcataatta cctctcgctt
     2221 ttctgcaata aaaaaggcgt catttctgac gcccgtt
//
LOCUS       DAJOWB010000292         2245 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJOWB010000292 DAJOWB010000000
VERSION     DAJOWB010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.296"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..396)
                     /locus_tag="OHO85_004958"
     CDS             complement(<1..396)
                     /locus_tag="OHO85_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HCQ2674169.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYV
                     FDVHHIMSILEYIFDHNAKPDIWDYFKAMGVGWLKTIIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSLNPVNGLKRIFGLKTVK"
     gene            complement(405..1172)
                     /gene="sctT"
                     /locus_tag="OHO85_004959"
     CDS             complement(405..1172)
                     /gene="sctT"
                     /locus_tag="OHO85_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HCQ2674170.1"
                     /translation="MEEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALFQHYHYELSTLNEIDIALFAAREIIIGLFIACLLSSPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGLNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTV
                     KSALAFLILIIYFGPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(1174..1434)
                     /locus_tag="OHO85_004960"
     CDS             complement(1174..1434)
                     /locus_tag="OHO85_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HCQ2674171.1"
                     /translation="MNDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1444..2109)
                     /locus_tag="OHO85_004961"
     CDS             complement(1444..2109)
                     /locus_tag="OHO85_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HCQ2674172.1"
                     /translation="MSNSISLIAILTLFTLLPFIIASGTYFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGTEIYYNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVIISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(2099..>2245)
                     /locus_tag="OHO85_004962"
     CDS             complement(2099..>2245)
                     /locus_tag="OHO85_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HCQ2674173.1"
                     /translation="IISLLPESEKNIEIRVNGALTGYGELVEVDDKLGVEIHSWLSGH
                     NNVK"
BASE COUNT          779 a          455 c          369 g          642 t
ORIGIN      
        1 ttttacggtt tttaagccaa atatacgctt taagccattt actggattca atgaatcaaa
       61 cttaaattta acagcttcag ttgctaattg cattttactt tgaaaccagg agacaagtat
      121 tgttgtgaac atgcaaacca gcaaaaaagg aatgatcgtt ttcaaccaac caacccccat
      181 agctttaaaa tagtcccaaa tatctggttt agcgttatga tcaaagatgt attcaagaat
      241 cgacataatg tgatgcacat caaaaacata tccgagatat aaagtaccca caagcattat
      301 tacagagatg gttaagtctc tactttttag aatttgccct tttttagaag catcctggag
      361 tttcttttgt gttggctttt cagttttatt ggccatgaaa cactctattt ctcaatgtat
      421 agttgcaatt gttctgggaa aaaggataac ggcattactc gttcagccag tattgggcca
      481 aaataaataa ttaatattaa aaatgccagc gcacttttga cagtcagtga aatggcaaaa
      541 gcatttagtt gtgaagcata acgtgccagc aatcccagga cagcctcacc gcccagcata
      601 acggcaatca cggggcttgc gtaaacgata gtatgagtca ttatgttatt aatatatgag
      661 aataaaggct ctaatttagg aaaattaaaa ttgcctgaag gccataaatt ataactttgc
      721 caaagagttt ccagaataaa gttcaatcca ccgttagtaa gataaaccgc tgcagagaat
      781 aggttaaata atcttgctaa ttccgaagta tcgacaccag tagcaggatc caaagtacta
      841 cttaacgttg caccacgttg gttatcgata aaactaccta tagcaaggaa aatccaaaac
      901 ggggatgata ataagcaagc aatgaacagc cctatgataa tttctctggc agcaaacaaa
      961 gcgatatcga tttcatttaa ggtcgataat tcatagtggt aatgctgaaa taatgcacat
     1021 gataccacta ttattattgg atgacggact actgttggta tattcccact ggcgaaaaat
     1081 ggtaacaaat agaaagttgg tgccaatcgg caaaaaccga gcgctgtagc agccagcaga
     1141 ctatgcaact gatagagtat cgcctcctcc atcctatcct ctcgcgaatg ctaaattcag
     1201 catttcgata ccaaacgaat ataatttttc accataccat ccgctcatta aaaaaaagca
     1261 aatacttacg cataataatt tgacgccaaa tggcaacgtc tgttcttgta actgtgttac
     1321 cgtttgaaaa agccctacca ataaaccaac aaatgtcgca actgcgatag gtcctgcaga
     1381 cataactaaa atcagataca gcgccctatt ccctgcaaaa acaatatcgt tcatattttt
     1441 tcctcaggga ttaatggata ggtcaaaata ttgtaaaatg agtccttttg ataacattgt
     1501 ccaaccgtcc atagcgacaa atagaattaa ttttatcggt gtagatatga ttaccgggct
     1561 catcatcatc atacctaacg ttaacagaac gctggatata accagatcca ctacgacaaa
     1621 gggtaaatag atataaaaac ctataataaa tgcacttttt atctcactca atgcatatgc
     1681 gggcagtaac gagaaaattg aaatattatc atcatcgatt atctcttcat tgtcttcaga
     1741 actattcacc ttctgaatct tttcaaaaaa gctaaccaac tcaggttcag aatattttat
     1801 caaatatgat ttataaccac tcatgcctgt ctcaacaaaa ttaacaaccg acgctacatt
     1861 attgaatgaa agattctcat tctgactgtt ataatatatt tccgtcccaa ccggcatcat
     1921 tacaaacatt gacaatagca aagcaacccc attaagtgtc atatttgatg gcacttgctg
     1981 aagccccagc gcgtttcgga caataacaaa aacaatcgag aatttaataa aatatgtccc
     2041 ggatgctatt ataaacggca atagtgtaaa taatgttagt atagctatca gcgaaatgct
     2101 atttgacatt attatgccct gataaccaag aatgaatctc tacgcccaat ttatcatcca
     2161 cttcaacaag ttcgccatag ccagttagcg cgccatttac acgtatttct atatttttct
     2221 cagattcagg aagcagagat attat
//
LOCUS       DAJOWB010000293         2225 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJOWB010000293 DAJOWB010000000
VERSION     DAJOWB010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.297"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..535
                     /locus_tag="OHO85_004963"
     CDS             <1..535
                     /locus_tag="OHO85_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCQ2674174.1"
                     /translation="QLPAFRQKLSDGWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHI
                     VPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGMTGILFSLGASMVL
                     GGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQE
                     ISTADEGDGGQVVVIGR"
     gene            596..>2225
                     /locus_tag="OHO85_004964"
     CDS             596..>2225
                     /locus_tag="OHO85_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCQ2674175.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTSVLDSDGNTNIAGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     AITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVK"
BASE COUNT          480 a          571 c          727 g          447 t
ORIGIN      
        1 acagctcccg gcgtttcgtc agaaactgag cgacggctgg tatcaggtac ggattgccgg
       61 gcgggacgtc agcacgtccg ggttaacggc gcagttacat gagactctgc ctgatggcgc
      121 tgtgattcat attgttccca gagtcgccgg ggccaagtca ggtggcgtat tccagattgt
      181 cctgggggct gccgccattg ccggatcatt ctttaccgcc ggagccaccc ttgcagcatg
      241 gggggcagcc attggggccg gtggtatgac cggcatcctg ttttctctcg gtgccagtat
      301 ggtgctcggt ggtgtggcgc agatgctggc accgaaagcc agaactcccc gtacacagac
      361 aacggataac ggtaagcaga acacctattt ctcctcactg gataacatgg ttgctcaggg
      421 caatgttctg cctgttctgt acggggaaat gcgcgtgggg tcacgcgtgg tttctcagga
      481 gatcagcacg gcagacgaag gggacggtgg tcaggttgtg gtgattggtc gctgatgcaa
      541 aatgttttat gtgaaaccgc ctgcgggcgg ttttgtcgtt tatggagcgt gaggaatggg
      601 taaaggaagc agtaaggggc ataccccgcg cgaagcgaag gacaacctga agtccacgca
      661 gttgctgagt gtgattgatg ccatcagtga agggccgatt gaaggtccgg tggatggatt
      721 aaaaagcgtg ctgctgaaca gtacgtcggt gctggacagt gatgggaata ccaacatcgc
      781 cggtgtcacg gtggtgttcc gggctggtga gcaggagcag actccgccgg agggatttga
      841 atcctccggc tccgagacgg tgttgggtac ggaagtgaaa tacgacacgc cgatcacccg
      901 cgccatcacg tcggcaaaca tcgaccgact gcgctttacc ttcggtgtgc aggcactggt
      961 ggaaaccacc tcaaaggggg accggaatcc gtcggaagtt cgcctgctgg ttcagataca
     1021 gcgtaatggt ggctgggtga cggaaaaaga catcaccatt aagggcaaaa ccacctcgca
     1081 gtatctggcc tcggtggtgg tggataacct gccgccgcgc ccgtttaata tccggatgcg
     1141 cagaatgacg ccggacagca ccacagacca gctgcagaac aaaacgctct ggtcgtcata
     1201 caccgaaatc atcgatgtga aacagtgcta cccgaacacg gcactggtcg gcgtgcaggt
     1261 ggactcggaa cagttcggca gccagcaggt gagccgtaat tatcatcttc gcgggcgcat
     1321 tctgcaggtg ccgtcgaact ataacccgca gacgcggcaa tacagcggta tctgggacgg
     1381 aacgtttaag ccagcataca gcaacaacat ggcctggtgt ctgtgggata tgctgaccca
     1441 tccgcgctac ggcatgggga agcgtctcgg tgcggcggat gtggacaaat gggcgctgta
     1501 tgtcatcggc cagaattgcg accagtcggt gccggatggc tttggtggca cggagccgcg
     1561 catcacctgt aatgcctacc tgaccacaca gcgcaaggcg tgggatgttc tcagtgattt
     1621 ctgctcggcg atgcgctgta tgccggtatg gaacgggcag acgctgacgt tcgtgcagga
     1681 ccgaccgtcg gataaggtgt ggacctataa ccgcagtaat gtggtgatgc cggatgatgg
     1741 cgcgccgttc cgctacagct tcagtgccct gaaggaccgt cataatgccg ttgaggtgaa
     1801 ctggattgac ccggataacg gctgggagac ggcgacagag cttgttgaag atacgcaggc
     1861 cattctccgt tacggtcgta acgtcacgaa gatggatgcc tttggctgta ccagccgggg
     1921 gcaggcacac cgcgccgggc tgtggctgat taaaacggaa ctactggaaa cgcagaccgt
     1981 ggacttcagc gtgggtgcgg aagggcttcg ccatgtgccg ggcgatgtca ttgaaatctg
     2041 cgatgatgac tatgcgggga tcagcaccgg cgggcgcgtg ctggcggtga acagccagac
     2101 ccggacgctg acgctcgacc gtgaaatcac gctgccatcc tccggtacca cgctgataag
     2161 cctggttgac ggaagtggca atccggtcag cgtggaggtc cagtccgtca ccgacggcgt
     2221 gaagg
//
LOCUS       DAJOWB010000294         2214 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJOWB010000294 DAJOWB010000000
VERSION     DAJOWB010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.298"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            135..1532
                     /locus_tag="OHO85_004965"
     CDS             135..1532
                     /locus_tag="OHO85_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCQ2674176.1"
                     /translation="MAIHDAGMFTVKEINRLKILQDVIERNLRPGQAPEMLGITPRHC
                     SRLLKRYRQSGPLGMNNQSRGRTGNRLLPASLTDEALSIIRERYRDFGPTLAREKLEE
                     VHGLILGKETIRRLMIKAGLWIPRRQRAPKIHQPRYRRPCTGELIQIDGCDHHWFENR
                     GRPCTALVYVDDATSRLMHLLFVKSESTFTYFEATRGYIEKYGKPMILYSDKASVFRV
                     NNKHATTGPGETQFARAMRCLNITPLCAETSQAKGRVERAHLTLQDRLVKELRLKGIS
                     TIDAANAFAEEYMADYNQRFAKAPRHDFNAHRPLALTDDLDAEFTWREPRRVSKNLTV
                     QYDKVLYLIEDSEYSRRAIGKYIDVWHYPDGHKELRLNDVLLPYSTYDRLSEVDPVAI
                     VDNKRLGHVLDVARQVQKKRDNNRSQSIPSGDEPSRRRHAPSINKPQRSLNEEDLLEA
                     MIQLQGSSEAIFGKR"
     gene            1764..2033
                     /locus_tag="OHO85_004966"
     CDS             1764..2033
                     /locus_tag="OHO85_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674177.1"
                     /translation="MKDKNFSRSISLQCPTCGTTTLLNDQDIEQVRCARCGREMTMNR
                     LIEENSENIHSHVNEIQKDLEKELIKNLSDKLKKAFKGNKNIRIK"
     gene            2033..>2214
                     /locus_tag="OHO85_004967"
     CDS             2033..>2214
                     /locus_tag="OHO85_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674178.1"
                     /translation="MTASLGDVFGGLAFFLSIYATIKTIKFNERQKALIEAQEKLTNL
                     QLTKEANELESSKRADV"
BASE COUNT          663 a          505 c          521 g          525 t
ORIGIN      
        1 atagattgtg cgcacaaaca ttgtagaggc tcaatgctaa tgtctatttg tggctcatta
       61 ggaatgtcca ggctatagtt tttcaaacct gtatgcctag tcaacgactc acttttcctg
      121 atgaggagtt ttttatggct atacatgacg ccgggatgtt taccgtgaaa gaaatcaatc
      181 gcctcaaaat acttcaggat gtcattgaac gtaacctgcg tcctggccaa gctcccgaga
      241 tgctcggcat cacgccacgt cattgcagcc gtctgctgaa acgttatcgt cagtccgggc
      301 cgcttggtat gaataaccag agtcgcggtc gcactggcaa ccgcctgttg cctgcgtcac
      361 taactgacga ggcactgagt atcatacgag aacgctaccg cgattttggc cccacgctgg
      421 cacgggaaaa actagaggaa gttcacggtc tgatcttggg caaggaaacc atccggcgtc
      481 tcatgataaa agccgggctc tggattcccc gtagacaacg tgcaccgaaa attcatcagc
      541 cccgttaccg gcggccatgt actggcgagc tgatacaaat tgatggctgt gaccatcact
      601 ggtttgaaaa ccggggtcgg ccatgtacgg cactggtcta tgtcgacgat gccaccagtc
      661 gtctgatgca cctgctgttt gtgaaatctg agtccacttt tacctatttt gaagcgacgc
      721 gcggctatat cgagaaatac ggtaaaccca tgatccttta cagcgataaa gccagcgtat
      781 tcagagtcaa taataagcac gccaccacgg ggcctggtga aacccaattt gcccgagcca
      841 tgcgctgcct caatattacc ccactatgcg cggagaccag tcaggcaaag gggcgcgttg
      901 aacgtgccca cctgacactt caggaccggc tagttaagga actcaggcta aaaggaatca
      961 gtacgataga tgcagcaaat gcgtttgctg aagaatacat ggccgattac aaccagcgat
     1021 ttgccaaagc cccacgacat gactttaatg cgcaccggcc actggcatta accgatgatc
     1081 ttgatgcgga atttacctgg cgtgagcccc ggcgggtttc aaaaaacctt actgtacagt
     1141 acgataaggt gctttatctg attgaggaca gcgaatacag ccgacgggct atcgggaagt
     1201 atatcgatgt ctggcactat cctgacgggc ataaggaact gcgcctgaat gatgtgctgc
     1261 tcccctattc cacctatgat cggttgtcgg aagttgaccc cgtcgcgatt gttgataata
     1321 aaagactggg tcatgtgctg gacgttgccc gccaggtcca gaaaaaaagg gataataatc
     1381 gatcccagtc gataccttca ggagatgagc cttcccgcag acggcatgct ccgagcatca
     1441 acaaacctca gcgatcgctg aacgaagaag acctgcttga agccatgata cagcttcagg
     1501 gtagctcgga ggccattttt ggtaaaaggt gaacttgacg ctaattataa aggaaatccc
     1561 ggactcactg accagtggcg atatgtgcgc tatgctggag tcattgcaat catgtcctga
     1621 gaatatttct gggaaatgca aataacttca atgatattat atacaaagat aaacgtcggg
     1681 aagctgggga aaatttcagt tttcgcaaac cacgaaccac ttcgcggtta aatatcaatc
     1741 aactggcgag agtagagtaa aacatgaaag ataaaaattt cagtagatct attagcttgc
     1801 agtgtcctac ttgcggtact accacactac tcaatgacca agacattgag caagttcgct
     1861 gtgctagatg cggcagagaa atgacaatga accgtctgat agaggagaat agcgaaaata
     1921 ttcattctca cgtaaatgag atacaaaaag atctagagaa agaattaata aaaaatcttt
     1981 ctgataaatt aaaaaaagcc ttcaaaggta ataagaatat aaggattaaa taatgacggc
     2041 atctctgggt gatgtgtttg gtggtttagc attttttctc tcaatatatg caacaataaa
     2101 aaccatcaag ttcaatgaaa gacaaaaggc attaattgaa gcacaagaaa aattaacaaa
     2161 ccttcagctt actaaagaag caaatgaact ggaaagctct aaaagagctg atgt
//
LOCUS       DAJOWB010000295         2171 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJOWB010000295 DAJOWB010000000
VERSION     DAJOWB010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.300"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(175..>2171)
                     /locus_tag="OHO85_004968"
     CDS             complement(175..>2171)
                     /locus_tag="OHO85_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCQ2674179.1"
                     /translation="DDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDG
                     LPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGAGSTAGYEQATAYTLT
                     GQLQSRHLNLPQLDCDYTWNDNGQLVRISGPQECREYRYSGTGRLTGVHTTAANLDID
                     IPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIPEGV
                     IRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTG
                     WMSLSRKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKARHR
                     SLAEVLQEDTGVTLPAELAVMLGRLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEA
                     EYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGEESAQHLQQSLRLPG
                     QQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGLKFHV
                     NGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMAIYWNSR
                     ASLFCSTELNSKSQSPALGLGHEFAHAQYYLLDKENFMALLSRTDKKYENKEEARVIT
                     IIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
BASE COUNT          427 a          695 c          489 g          560 t
ORIGIN      
        1 taagaccttc cttgctaagc ggaactaacg tggtaacata cgatggaaga ttaattatct
       61 ctatcgtttt cacgctatct attccacaca attcattcac tcgccccatc cccagtaccg
      121 aaccagaaaa tagagacaat aaaaccatca aatatttcat cataaaaaac ctcactattc
      181 tggcgtaccc gttattttca tcgtctgcaa cggtccatcc acccgataaa aaggcagtcc
      241 actatgagct cctctggtac actcacctaa agttttcgcc gcacgcgatt caattattgt
      301 aataaccctt gcctcttctt tattctcata tttcttatct gttcggctta aaagagccat
      361 gaaattttcc ttatcaagaa ggtaatactg cgcatgagca aactcatgtc ccaaacctaa
      421 tgcaggcgat tgtgatttcg agtttaattc agttgaacaa aataaagatg ctctactatt
      481 ccaatatatc gccatattat tcgaattaaa tctaacattg gttccttcaa tatactctat
      541 atttatagtt tcttcagagc ttgataaaaa atctatagtt tctttcattt gtgaatcttg
      601 ttttaaatac tcaacagcct gattaaaatc acttggatca ccgttaacat ggaattttaa
      661 acccaaagaa tctataaacc gaacagggtt taatggatat ttatacagat tccattcacc
      721 tctcagccct atcgggtctt gggtgatata tctcccctgc aacggatcat agtaccggtt
      781 gcggttgtag tacagccccg actcctcatc atactgctgt cccggcagac ggagtgactg
      841 ctgaaggtgc tgcgcgcttt cctcgcccag caggtttccc cactcgtcat actccccctg
      901 ccacgccgtt tccccttccg ggctgataag cgccagcggc agcccacggt ggtcacagtg
      961 gtaaagatga agtttcctct ccgggatgta ttccgcctcc agttgcgcgg ccatctgttc
     1021 cgccgtcagc ccgcactgcg caagccactg ctggctttct tcactcacgc tgccctgccg
     1081 cagttcccgc tccagcctcc ccagcatcac cgccagctcc gccggtagcg tcacacccgt
     1141 gtcctcctgc aacacctccg ccaggctacg gtgccgcgcc ttcgcctgtt caccattctc
     1201 tgtttcgatt ctcaggagcg gcgtgaagct tcccggctgg tataccgtct ggattcttgt
     1261 ctgttgggtc tgtacagtgg tcagcctgtc accgtcccac ccgtaccagg tctcctccgg
     1321 tttacgcgac agcgacatcc accccgtcag gtcacgctcc cgccgccaca cccgttttcc
     1381 cgtccggcgg cccagcgggt catagaggta gcggctctcc acctgcggct caccgtgctg
     1441 tatccgcgtg tagaacacca gacggtgctg gctgtcgtag tggtagtggt gggtccgctc
     1501 gtcgtgcatc cgtatcaccc cttccgggat gcggtccgtc ttctccgcca gcctgccgta
     1561 ttcatcgtag cggtagacat agtgcgcatc ttccgcgatg cggttatccg gccacgccgt
     1621 gagggtgctg tccgggtgca gttccgggtc cggcagccgg tttcctgccg ggtccgtcgc
     1681 atacgggata tcgatatcca ggttcgctgc cgtagtgtgg acgcccgtca gccttcccgt
     1741 gccgctgtaa cggtactccc ggcactcctg cgggccgctg atgcgcacca gttgtccgtt
     1801 atcgttccac gtatagtcac agtcaagctg cggaaggttc aggtgcctgc tctgtagctg
     1861 ccctgtgagg gtgtacgccg tggcctgttc gtaccccgct gtactgcccg ccccgccgaa
     1921 gctacgggcc gtctcgcggt gcagccggtc ccgcgtgtac tcgaccagcg gcgttccgcc
     1981 cagcttcatc cccgcaagat aaccgctgcc atacgtcagc cactccaccg gcggcagacc
     2041 gtccggctcc tgacgggtcg ccagcccctg ttcgctgtac gcatgccccg tctcatgctc
     2101 ccacagcatc tcccctgtct ccgggttctc caccgtctgc cgttcacccg tcaggcggcc
     2161 tttatcgtca t
//
LOCUS       DAJOWB010000296         2136 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJOWB010000296 DAJOWB010000000
VERSION     DAJOWB010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.301"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            8..910
                     /gene="hlyE"
                     /locus_tag="OHO85_004969"
     CDS             8..910
                     /gene="hlyE"
                     /locus_tag="OHO85_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_131199601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HCQ2674180.1"
                     /translation="MVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKEL
                     SRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYN
                     EKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKS
                     SYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQSF
                     FTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAK
                     KMINTCNEYQKRHGKKTLFEVPEV"
     gene            complement(1117..1563)
                     /locus_tag="OHO85_004970"
     CDS             complement(1117..1563)
                     /locus_tag="OHO85_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCQ2674181.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRDNLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1655..>2136)
                     /locus_tag="OHO85_004971"
     CDS             complement(1655..>2136)
                     /locus_tag="OHO85_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCQ2674182.1"
                     /translation="PSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDLTL
                     RDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQGTT
                     ADMIHKIIPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRVL"
BASE COUNT          631 a          490 c          471 g          544 t
ORIGIN      
        1 gactgaaatc gttgcagata aaacggtaga agtagttaaa aacgcaatcg aaaccgcaga
       61 tggagcatta gatctttata ataaatatct cgatcaggtc atcccctggc agacctttga
      121 tgaaaccata aaagagttaa gtcgctttaa acaggagtat tcacaggcag cctccgtttt
      181 agtcggcgat attaaaacct tacttatgga tagccaagat aagtattttg aagcaaccca
      241 aacagtgtat gaatggtgtg gtgttgcgac gcaattgctc gcagcgtata ttttgctatt
      301 tgatgagtac aatgagaaga aagcatccgc ccagaaagac attctcatta aggtactgga
      361 tgacggcatc acgaagctga atgaagcgca aaaatccctg ctggtaagct cacaaagttt
      421 caacaacgct tccgggaaac tgctggcgtt agatagccag ttaaccaatg atttttcaga
      481 aaaaagcagc tatttccagt cacaggtaga taaaatcagg aaggaagcgt atgccggtgc
      541 cgcagccggt gtcgtcgccg gtccatttgg tttaatcatt tcctattcta ttgctgcggg
      601 cgtagttgaa gggaaactga ttccagaatt gaagaacaag ttaaaatctg tgcagagttt
      661 ctttaccacc ctgtctaaca cggttaaaca agcgaataaa gatatcgatg ccgccaaatt
      721 gaaattaacc accgaaatag ccgccatcgg ggagataaaa acggaaactg aaacaaccag
      781 attctatgtt gattatgatg atttaatgct ttctttgcta aaagaagcgg ccaaaaaaat
      841 gattaacacc tgtaatgagt atcagaaaag acacggtaag aagacactct ttgaggtacc
      901 tgaagtctga tcagcgatta ttctctccat gtactcaagg tataaagttt atcacattta
      961 aaaatacaga cgttttttaa agattgagag ttacctgttg ataataatgt atcgggtaac
     1021 tctcattatg acagagcgct ttcaaaaaca gggctaggaa atggcgagca tccagacgta
     1081 tattaagaaa gcccccatca gcatctatta aatttatcac tgcgcccagt caggtttatt
     1141 taagatatga tcctgccagt caatcacttc tgattctttc actgcgatat gacgcacaga
     1201 gatacgttca ccatgcattg ccgctttcga accagtaagt agcggatgcc acgcaggtaa
     1261 atctttacct tctgccagca aacgataagc gcaggtcatt ggcagccatt cgaatgttgg
     1321 cagattatca cgggttaatt taatgcagtc gggttcaaac tcaaaacgac gttcgtagtt
     1381 ccgacattga caggttttaa tattgagctg gcgacaggcg acgttagtga agtagatttc
     1441 gtcggtgtct tcatccatca gtttatgcag gcaacactga ccgcaaccat cacacaacga
     1501 ctcccattcc gcatcgctca tttcgtccag ggttttactt tgccagaaag gtacatcgct
     1561 catcaggtgt tccgccatta cgttaaaacg caccttataa ccagtctggc acagcgatgc
     1621 aagttttgcc gccgctttca ggcggcaaaa agtattacaa aacgcgagtt gtcaaagaat
     1681 gcccatcgaa agtgactgtc agctcatcac cgctttgcaa cgggccgacg ccatcaggcg
     1741 tgcctgtcaa cacaacgtca ccggccttga gggtaaaaaa cttgctcata taagcgatca
     1801 gcggaatgat tttatggatc atgtccgcag tcgtaccttg ctggcgttgt tcgccgttta
     1861 cgctcaggcc cagcgttgta ttttgcggat cgccggtaaa ttccgccgcg ggaataaacc
     1921 cggaaagcgg acaagagtta tcaaacgctt tagccttttc ccacggctgc ccggctttct
     1981 tcatttttcc ctgaacatca cgcaatgtca gatcaagcgc cacgccgtaa ccggcaatgg
     2041 ctttgcggac atgctcttcc gtagcctgac gcagcgtcgc gccaatcaac accgccagtt
     2101 cgacttcatg atgaactgaa ccgaaatcgg atggga
//
LOCUS       DAJOWB010000297         2134 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJOWB010000297 DAJOWB010000000
VERSION     DAJOWB010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.302"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            369..524
                     /gene="hokD"
                     /locus_tag="OHO85_004972"
     CDS             369..524
                     /gene="hokD"
                     /locus_tag="OHO85_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCQ2674183.1"
                     /translation="MKQQKAILIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVTVF
                     TAYESER"
     gene            complement(641..1150)
                     /locus_tag="OHO85_004973"
     CDS             complement(641..1150)
                     /locus_tag="OHO85_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCQ2674184.1"
                     /translation="MGHNNTKGNRKFIKGRYTANAAKGERLVSSEFQLTFAGHEDISV
                     LVRTSQIPEMTREDVEDYGPNGVKFNQHGPIRNSGEIQVQCVETIEGDILQFIKDRIA
                     AKDYVDITMAATPESKSSGVNAVTKAATTIEMLDCKIYSDAIDFSTEDVTAAVRPSLR
                     IVYNWIEWD"
     gene            complement(1162..1743)
                     /locus_tag="OHO85_004974"
     CDS             complement(1162..1743)
                     /locus_tag="OHO85_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674185.1"
                     /translation="MAPIPYGVYSQADGVSPFLKVTLTNSQYQVTGYISQGAAMNMAQ
                     NWEAPFTGMSMGSVAGAFSGFAQVGTETTSVARWNSLMVWEGGTPPTFTLPVTFIALF
                     DPFTEVSGAIAALSAMISPELKDASIGGRIPERVTLNIGRRINIIDVAIQDISFDLDA
                     PRDSNGHFLKNTVNLQLTGSSIYNSSDIVRAFQ"
     gene            complement(1779..>2134)
                     /locus_tag="OHO85_004975"
     CDS             complement(1779..>2134)
                     /locus_tag="OHO85_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674186.1"
                     /translation="FLAACGVDDDLIEALADDEDDDAAADVGASIAGLDSDDRDELEA
                     AFVVAGTSDEMLTEAFKKVVRNGEIKLIRKRLRKKRLTAAQKSALKKARRKAQTGAAK
                     LARKKSMKLRRKRLG"
BASE COUNT          549 a          545 c          498 g          542 t
ORIGIN      
        1 ctgtgtatac atgttcagcc gaaaaatgct tgtcgatatc gaccgcaaag aacaagcaga
       61 ggagatctta gtatggattt tctttggcgc ggtctggcca ttagggatca tgtttgctgc
      121 aacatttctt ctgatgtgga tattcaccct tccaggtgat ttctatagaa aaaaagccag
      181 acattgatac aatcgttgcg ggtgcttgag gctatctgct tcaggcatta cccgaaaagc
      241 agatagaaga aagccccaga taacattacg cgtcctgcaa gacgcttaac attaatctga
      301 ggcaatatct atgcttagca tacgtagatt agcctcttac cgaccaaaag gtcaaggaga
      361 agcaggctat gaagcagcaa aaagcgatat taatcgctct gatcgtcatc tgtttaaccg
      421 tcattgtgac ggcactggta acgaggaaag acctctgcga ggtacgaatc cgaaccggcc
      481 agacggaggt cactgtcttc acagcctacg aatctgaaag gtaagagacc tggcggggag
      541 agatcctcgc cactcttcgt gtgtcaggta tcctcaatgc accctttcct ctccaaataa
      601 aaaagctccc gaaggagctt taaaatacaa gggatgactc ttaatcccac tcaatccagt
      661 tgtagacgat acgaagtgac gggcgcacag cggcagtcac atcttcggta ctaaagtcga
      721 ttgcatcact gtagattttg cagtccaaca tttcaattgt tgtagcagct tttgtcacag
      781 cgttaacccc ggaagatttg gattcagggg tagcagccat cgtgatatca acatagtcct
      841 tcgccgcaat gcgatcttta atgaactgaa gaatatcgcc ttcgatagtc tccacgcact
      901 ggacctggat ttccccagag tttcgaattg gaccgtgctg gttgaacttc acaccattcg
      961 gaccatagtc ctccacatcc tcgcgggtca tttcaggaat ttgcgacgtg cgaaccagta
     1021 cgctgatatc ttcatggcct gcaaaagtga gctggaattc agaagatacc agtcgttcgc
     1081 ctttggccgc gttggcagta tagcggccct taataaattt acggtttccc ttagtgttat
     1141 tgtgccccat ataaaatcct tttactggaa cgcccgaaca atatcggagc tgttatatat
     1201 cgaagaaccg gtcaactgga ggttgacggt gtttttcagg aaatgtccat tgctgtccct
     1261 gggcgcatcg agatcgaaac ttatgtcctg gatagcgaca tcaatgatgt tgatccggcg
     1321 accaatgttt agcgtcacac gctccgggat tcgaccacca atactggcat ctttaagttc
     1381 cgggctaatc atcgctgaca atgcggcgat agctcctgaa acctccgtga atgggtcaaa
     1441 caaagcgatg aaagttactg gcagcgtgaa agtcggcggt gttcccccct cccaaaccat
     1501 taagctgttc caacgggcca ccgacgttgt ttcagtacca acctgcgcaa aaccactgaa
     1561 agcaccagca acagacccca tggacatacc ggtaaacggc gcttcccaat tctgggccat
     1621 gttcattgct gccccctggc tgatatatcc ggtaacctgg tactgagagt tcgttaaagt
     1681 aactttcaga aatggcgata caccgtcagc ctggctgtaa accccataag gtataggtgc
     1741 cattcaagtt aaaggccgga gttctccggc ctcctccttt agccaaggcg cttacggcgc
     1801 agtttcattg actttttgcg ggcaagtttt gccgcgccgg tctgggcttt tcgacgcgct
     1861 tttttcagcg ccgatttttg agccgcagtc agacgttttt tacgcaggcg tttacggatg
     1921 agtttgatct caccgttacg aacaaccttc ttaaatgctt cagtcagcat ttcatcagaa
     1981 gtgccagcaa caacaaacgc cgcttccagt tcgtcgcggt cgtcgctatc taaaccagcg
     2041 atagaggcac caacatcagc agcagcgtcg tcgtcttcat cgtcagccag tgcttcgatc
     2101 aggtcatcat ctacaccgca tgctgcgagg aagt
//
LOCUS       DAJOWB010000298         2120 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJOWB010000298 DAJOWB010000000
VERSION     DAJOWB010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.303"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1367
                     /locus_tag="OHO85_004976"
     CDS             <1..1367
                     /locus_tag="OHO85_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCQ2674187.1"
                     /translation="YGRLAEKTDRIPEGVIRMHDERTHHYHYDSQHRLVFYTRIQHGE
                     PQVESRYLYDPLGRRTGKRVWRRERDLTGWMSLSRKPEETWYGWDGDRLTTVQTQQTR
                     IQTVYQPGSFTPLLRIETENGEQAKARHRSLAEVLQEDTGVTLPAELAVMLGRLEREL
                     RAGAVSAESEAWLAQCGLTVEQMESQMEAEYIPERRLHLYHCDHRGLPQALISPEGET
                     AWRGEYDEWGNQLNEENPPHLYQPYRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDP
                     IGLKGGINLYTYPLAPIRYTDPLGLERVISVYGPPAPDRAGAETPLVLTDMTGGVTIY
                     YDPETGDSMTFDCSNRIDRRSQRGAGDPYTGEVVGCETNGSGISAAYGTTKIYTTDTR
                     ARWLHGGGSSLRDPYAPRQGWKPTMGCTRAQNEDVDELCKKVTSWMYSHPGERIRYER
                     FKTR"
     gene            1345..1737
                     /locus_tag="OHO85_004977"
     CDS             1345..1737
                     /locus_tag="OHO85_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674188.1"
                     /translation="MNVLRHVNAINMGVCILILVIGCANAKVLKRCILPAPDGGVEKL
                     SKPNLHGVIKKVDLNTNEAEIKIKEGGAVSFIFNEETLYFTVFGGDFIMDDLKIVKNT
                     EAWVWYKNCDSKNKKVAVELLQIYELHQ"
BASE COUNT          601 a          416 c          624 g          479 t
ORIGIN      
        1 aatacggcag gctggcggag aagacggacc gcatcccgga aggggttatc cggatgcacg
       61 acgagcggac ccaccactac cattacgaca gccagcaccg tctggtgttc tacacgcgga
      121 tacagcacgg cgaaccacag gtggagagcc gctacctcta tgacccgctg ggccgccgga
      181 cgggaaaacg ggtatggcgg cgggagcgtg acctgacggg gtggatgtcg ctgtcgcgta
      241 aaccggagga gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac
      301 agacaagaat ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa
      361 cagagaatgg cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg
      421 acacgggggt gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac
      481 tgcgtgcggg tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg
      541 tggagcagat ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt
      601 accactgtga ccaccgggga ctgccgcagg cgcttatcag cccggaaggg gaaacggcgt
      661 ggcgcgggga gtatgacgag tgggggaacc agcttaatga ggagaacccg cctcacctgt
      721 accagccgta ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc
      781 gtcaccggta ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga
      841 aggggggaat aaatctatac acgtatccac tggcaccaat acgttacact gatccactgg
      901 ggttagaacg agttataagt gtatatgggc ctcccgctcc agaccgggca ggtgctgaaa
      961 ctcctcttgt tcttactgat atgactggag gagtgactat ttattatgat cctgaaacag
     1021 gtgattcaat gacttttgat tgtagcaata gaattgatcg tcgttctcaa cgtggtgctg
     1081 gtgatccata tacaggtgaa gtcgttggtt gtgagacaaa tggatcagga atatcggcag
     1141 catatggcac aacgaagatc tatactacag atactagagc aagatggcta catggtgggg
     1201 gatcatcgtt gcgcgatcct tatgctcctc gacaaggctg gaaaccaact atgggatgta
     1261 cccgtgcaca gaatgaagat gtagatgagt tgtgtaagaa agtgacaagt tggatgtatt
     1321 cgcatcctgg agagagaatt agatatgaac gttttaagac acgttaatgc gattaacatg
     1381 ggagtgtgca ttctaatttt ggttattgga tgtgcaaatg ctaaagttct taaacgatgt
     1441 atattacccg ctcctgatgg tggcgtagaa aaactatcaa aacccaactt acatggagta
     1501 attaaaaaag ttgatttgaa cacaaatgaa gcggaaatta aaataaaaga ggggggggca
     1561 gtgagtttta tttttaatga ggaaacattg tattttactg tttttggtgg ggattttatt
     1621 atggatgatt taaaaatagt aaaaaatacg gaagcatggg tatggtataa aaattgcgat
     1681 agtaaaaata aaaaagtggc agttgaactt cttcaaatct acgaattgca tcaatgaaaa
     1741 atatagttaa atagaggagg ttattaatag cagggaaagc atcacgtgat gctgattttt
     1801 tgcactaaat aattcgcgct aatgttcaat agttaatata tccctcatca gggaaagatc
     1861 acggtgtgcc gtaggcacaa agatgcaaaa cgggacgttt tgcatgtaac atgtaataag
     1921 cataagcttg tgaagagatg gtggcaggcc caccgggatc tgctgtcgga agtcggtccc
     1981 aaacaacccc ggctgctgtg gtaaagagcc atggtagcag ggaaagatca tcaggagttc
     2041 accagggaaa gatcacgtaa cgctactttt ttgtactaaa taattcgcat tttatgttta
     2101 aaaattgaga tattccttat
//
LOCUS       DAJOWB010000299         2118 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJOWB010000299 DAJOWB010000000
VERSION     DAJOWB010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.304"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            170..1546
                     /gene="ygeH"
                     /locus_tag="OHO85_004978"
     CDS             170..1546
                     /gene="ygeH"
                     /locus_tag="OHO85_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HCQ2674189.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYDFTPESIYR
                     AMTIFDRLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDIT
                     TVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVHFHNEKIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLK
                     QLTRICMR"
     gene            1714..1932
                     /locus_tag="OHO85_004979"
     CDS             1714..1932
                     /locus_tag="OHO85_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674190.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMAPFQNDMYSEENPISPYYKIEW"
BASE COUNT          772 a          365 c          344 g          637 t
ORIGIN      
        1 aggctatttc agattacatc actgaattcc taagataaat tagtgaacac aaccatagat
       61 atgtcgaatg taatttcctg tagcattaat gacacataaa tatgaatagc cataatttct
      121 attgcttaag caggatgcaa gaaaccaatt ttttcataga ggttaactaa tggacttaga
      181 aaataaattc tcatatcatt ttcttgaggg attaacgctc acggaagatg gaattcttac
      241 tcaaggaaat gagcaagttt atattccaca gaaagagtta ggtgtattaa tagtattact
      301 tgaatccgct ggtcatgttg tactgaaaga tatgatcatc gaatcagtat ggaaaaatat
      361 tattgttagt gacgagtccc tgacaagatg tatctattct ttgcgctgca tttttgaaaa
      421 aattggctat gatcgttgca tagaaacaat ctaccggaaa ggttatcgtt tcagcgggca
      481 ggttttcaaa actaaaatta atgaagataa tacttcagac tattccatag ctatattccc
      541 tttcactact tcattgaata cactggatcc attaatactt aatcaggaat tagtgcaaat
      601 catttcaaat aaaaaaatcg atggtctcta tacctatccg atggctgcga caaatttttg
      661 taatgatcac atatctcaaa attcattctt gagcagattc aagccagatt atttcgttac
      721 aggaagaata aaccagaata atgcagtgaa cactttatac attgagttga tcgacgctaa
      781 aaaccttttc ctcatcgcca gtaatcatct ccctgttgat gaactacata atacatcaca
      841 atttattata gataatatcc ttcaaacggt acataaacca gaacgatctg taagattagc
      901 taagcaggac caaggatata agaatcatta tttatcagat gaaatgttag ccggaaagaa
      961 agaactttac gacttcaccc ctgaaagcat ttacagggcc atgactatat ttgatagatt
     1021 acaaaataaa agtgatatac agacgctaaa aacagaatgt tattgccttc tagcggaatg
     1081 ccatatgtct ttggcacttc atggaaaaag tgaacttgaa cttgctgctc aaaaagcatt
     1141 agagctttta gattatgtat cagacataac cactgtcgat ggaaaaattt tagctattat
     1201 gggactgata actggtctgt ctggacaagc aaaagtatct catatcttat ttgaacaggc
     1261 taagatacac tcaactgata tagcctctct ctactactat agggcactag tccactttca
     1321 taatgaaaaa attgaagagg caaggatttg tatagacaaa tcactacaac tcgaacccag
     1381 aagacgaaaa gcagttgtga taaaagaatg tgtagatatg tatgtgccta acccgctcaa
     1441 aaacaacatt aaactctact ataaagaaac tgagagtgaa agccatcgag ttataattga
     1501 caacattttg aaattaaagc agctgacgag aatttgtatg cgataaattt ttattgacgc
     1561 aattttatta tttagatgta tgcaacttgt tatttaaaca acagagataa aaatatctaa
     1621 gatattcacc ttattgcaag atatttaaaa tgctctagag ttaaaagtat gatgtaaaga
     1681 ccattggcct ggacaattaa aaaagggagc tttatgacta atccaatcgg tatcaataat
     1741 ttatctcaaa gttcaaatat agcgaatgca acgggtgatg aagtcgtatc attagataaa
     1801 cacatcaaca ccagtgctac tgatacagat caaatacaag catttatagt ttcaacatgg
     1861 atggcgcctt ttcaaaatga tatgtattca gaagagaatc ctatctcacc ttactataaa
     1921 attgaatggt agacgattga ccaaataaat cataacgaga tacttttata atagtatctc
     1981 gttcctttca ttcaatcctc acacatgaaa aaatatgcgc tcaccaaaag ttaaattctt
     2041 aaccatcttc acgttttgta tatttattac aaaaatgtct ttcgccagca actcttgctc
     2101 taatgaagca ggaacaat
//
LOCUS       DAJOWB010000300         2093 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJOWB010000300 DAJOWB010000000
VERSION     DAJOWB010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.305"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            8..868
                     /locus_tag="OHO85_004980"
     CDS             8..868
                     /locus_tag="OHO85_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCQ2674191.1"
                     /translation="MFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALPAGLLLVMI
                     VRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLMVVFISAAL
                     LGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGTVLTRKWQP
                     PVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAGLTYFLWFR
                     GISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWLGQRSNRTP
                     RARIACRKSP"
     gene            complement(900..2081)
                     /gene="tet(A)"
                     /locus_tag="OHO85_004981"
     CDS             complement(900..2081)
                     /gene="tet(A)"
                     /locus_tag="OHO85_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_124470450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCQ2674192.1"
                     /translation="MIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHYGILLA
                     LYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIGRIVAGI
                     TGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPFFAA
                     AALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIMQL
                     VGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALML
                     GMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRAD
                     R"
BASE COUNT          407 a          646 c          647 g          393 t
ORIGIN      
        1 cgatttattg ttcacagcga tagcacctgc catttggggc agcacctaca ttgtcaccac
       61 ccaatacctg ccgaacttct caccgatgac ggtcgcgatg ctgcgggcgt tgccggcggg
      121 tttattgctc gtgatgatcg tccgacagat tccaacggga atctggtgga tgcgcatctt
      181 catcctcggc gcacttaata tttcgctatt ctggagcttg ttgtttattt cggtctaccg
      241 cctgccgggc ggggtcgcgg cgacggtagg cgctgtgcag ccgctgatgg tcgtgttcat
      301 ctctgccgct ctgctaggta gcccgatacg attgatggcg gtcctggggg ctatttgcgg
      361 aactgcgggc gtggcgctgt tggtgttgac accaaacgca gcgctagatc ctgtcggcgt
      421 cgcagcgggc ctggcggggg cggtttccat ggcgttcgga accgtgctga cccgcaagtg
      481 gcaacctccc gtgcctctgc tcacctttac cgcctggcaa ctggcggccg gaggacttct
      541 gctcgttcca gtagctttag tgtttgatcc gccaatcccg atgcctacag gaaccaatgt
      601 tctcggcctg gcgtggctcg gcctgatcgg agcgggttta acctacttcc tttggttccg
      661 ggggatctcg cgactcgaac ctacagttgt ttccttactg ggctttctca gcccggggac
      721 cgccgtgttg ctaggatggt tgttcttgga tcagacgctg agtgcgcttc aaatcatcgg
      781 cgtcctgctc gtgatcggga gtatctggct gggccaacgt tccaaccgca ctcctagggc
      841 gcgtatagct tgccggaagt cgccttgacc cgcatggcat aggcctatcg tttccacgat
      901 cagcgatcgg ctcgttgccc tgcgccgctc caaagcccgc gacgcagcgc cggcaggcag
      961 agcaagtaga gggcagcgcc tgcaatccat gcccacccgt tccacgttgt tatagaagcc
     1021 gcatagatcg ccgtgaagag gaggggtccg acgatcgagg tcaggctggt gagcgccgcc
     1081 agtgagcctt gcagctgccc ctgacgttcc tcatccacct gcctggacaa cattgcttgc
     1141 agcgccggca ttccgatgcc acccgaagca agcaggacca tgatcgggaa cgccatccat
     1201 ccccgtgtcg cgaaggcaag caggatgtag cctgtgccgt cggcaatcat tccgagcatg
     1261 agtgcccgcc tttcgccgag ccgggcggct acagggccgg tgatcattgc ctgggcgagt
     1321 gaatgcagaa tgccaaatgc ggcaagcgaa atgccgatcg tggtcgcgtc ccagtgaaag
     1381 cgatcctcgc cgaaaatgac ccaaagcgcg gccggcacct gtccgacaag ttgcatgatg
     1441 aagaagaccg ccatcagggc ggcgacgacg gtcatgcccc gggcccaccg gaacgaagcg
     1501 agcgggttga gagcctcccg gcgtaacggc cggcgttcgc ctttgtgcga ctccggcaaa
     1561 aggaaacagc ccgtcaggaa attgaggccg ttcaaggctg ccgcggcgaa gaacggagcg
     1621 tggggggaga aaccgcccat cagcccaccg agcacaggtc ccgcgaccat cccgaacccg
     1681 aaacaggcgc tcatgaagcc gaagtgccgc gcgcgctcat cgccatcagt gatatcggca
     1741 atataagcgc cggctaccgc cccagtcgcc ccggtgatgc cggccacgat ccgcccgata
     1801 tagagaaccc aaaggaaagg cgccgtcgcc atgatggcgt agtcgacagc agcgccggcc
     1861 agcgagacga gcaagaccgg ccgccgcccg aaacgatccg acagcgcgcc cagcacaggt
     1921 gcgcaggcaa attgcatcaa cgcatacagc gccagcagaa tgccatagtg ggcggtgacg
     1981 tcgttcgagt gaaccagatc gcgcaggagg cccggcagca ccggcataat caggccgatg
     2041 ccgacagcgt cgagcgcgac agtgctcaga attacgatca ggggtctgtt ggg
//
LOCUS       DAJOWB010000301         2030 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJOWB010000301 DAJOWB010000000
VERSION     DAJOWB010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.306"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            573..1445
                     /locus_tag="OHO85_004982"
     CDS             573..1445
                     /locus_tag="OHO85_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCQ2674193.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            1817..>2030
                     /locus_tag="OHO85_004983"
     CDS             1817..>2030
                     /locus_tag="OHO85_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCQ2674194.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNGGTLANH"
BASE COUNT          520 a          535 c          494 g          481 t
ORIGIN      
        1 acactcaact aataatatct cagcagatat tatcctgacc aggacacctc acattactga
       61 attctcttcc gacagtatca ctgcacaaat aaaaagaacg ggataaatat tcagaccgga
      121 agaatggtgt cacaaaagca tttaatcatt acgcatgacc aggaagggaa acacttaacc
      181 acatggtatc aacagatatc attacgtgtg cactcttata aaaaacacac agaagaagta
      241 agataattat aaacgtaaat ttaatatctc ccactgacat ctcccccaca atacactaaa
      301 aaatactcac agcagtaaac acagatacac ctgataatca tacagccgat aaatattttc
      361 ccctttacgg agaaatcagc ctgctgttta acaataacgg cgtattttca tggaaggaga
      421 tcacccagta acggataagt cctgatctcc ttcatgttaa tacgcaacaa tcccatcgat
      481 tcaaaacaat ttcagacaga tattatctcc gtgaatcccc cgccaccttt ccggtgcgcg
      541 gggttttgtc ttttttcacc gggaatacat gtatgaatcc gtctgatgcc attgaggcaa
      601 ttgaaaaacc gctctcctcc ctgccttact cactttcccg tcacatcctg gaacatctgc
      661 gcaaactcac cagtcacgaa cccgtgattg gcattatggg taaaagcggg gccggtaaat
      721 cctcactctg taatgcactg tttcaggggg aggtcacccc ggtcagtgat gttcacgccg
      781 gcacccggga agtgcggcgc ttccgtctga gtggccacgg tcacagcatg gttatcactg
      841 acctgcccgg ggtgggcgag agccgggaca gggatgcaga gtatgaagcc ctgtaccgtg
      901 acattctgcc cgaactggac ctggtgctgt ggctgattaa agccgatgac cgcgccctgt
      961 ctgtggatga gtatttctgg cgacacatcc tgcaccgggg acatcagcag gtgctgtttg
     1021 tggtgacgca ggccgacaaa acggagccct gccatgaatg ggatatggcc ggcattcagc
     1081 cttctcccgc acaggcacag aacattcggg agaaaacgga ggcggtattc cgtctgttcc
     1141 ggcccgtaca tccggttgtt gccgtatcgg cccgcaccgg ctgggaactg gatacgctgg
     1201 tcagtgcgct aatgacagca cttcccgacc atgccgccag tcccctgatg acccgcctgc
     1261 aggacgagct gcgcacggaa tctgtccgcg ctcaggcccg tgaacagttt accggtgcgg
     1321 tggaccggat atttgacacg gcggagagcg tctgtgttgc ctctgttgcc cgtacggttc
     1381 tgcgtgccgt ccgtgacacg gtggtctctg ttgcccgcgc tgtatggaac tggattttct
     1441 tctgaacagt ctgccaggga aatgcctgat aacattacgt taaccaactg aaaacatgac
     1501 acaaacccct catactcctc ctgtttctct tgcgtgaccg ccttgtcgtt aatagaataa
     1561 aacgatcgat aaaacaggta tcgatcgttg ctatcgatcg tttatagtgg ttaatggtct
     1621 gcgaaaaggc agctgaatct cttcatcatg cagaacggaa ttgcacacaa cggactgata
     1681 cttctctggc ggatgagaga ggggaagcat ttatgtccgg gaaaaaccac tttatctgag
     1741 catgctgttt tgtacctgcc ggtatccaca tttgtgggta ccggcttttt tattcaccct
     1801 ctgtaaggaa aagctgatga aacgacatct gaacaccagc tacaggctgg tatggaatca
     1861 cattacgggc accctggtgg tggcctccga actggcccgc tcacggggaa aacgcgctgg
     1921 tgtggcggtt gcgctgtctc ttgctgctgt cacatcagtc ccggcactgg ctgctgacaa
     1981 ggttgtacag gcgggagaaa ccgtgaacgg cggaacactg gcaaatcatg
//
LOCUS       DAJOWB010000302         1999 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJOWB010000302 DAJOWB010000000
VERSION     DAJOWB010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.308"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..1007)
                     /locus_tag="OHO85_004984"
     CDS             complement(<1..1007)
                     /locus_tag="OHO85_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS481-like element ISKpn28 family transposase"
                     /protein_id="HCQ2674195.1"
                     /translation="MLMQHIGVGYFGYYRATAYAMKHSLMPEIAKLRMKALNFWDKHG
                     IRAAADAFDVSTRTLYWWRRLLRTGGPEALIPRSKAPLVRRSRHWHPDVLKEIRRLRT
                     ELPNLGKEQIFVRLKPWCEARHFTCPSTSTIGRIIAGAHDKMRMIPVRLSARGKARLI
                     KKRSVKPRRPKQYRPVKTGELIGMDAIELRMGDLRRYIITMIDEHSDYALALAVPSLN
                     SDITSHFFSKATKLFPVAIRQVVTDNGKEFLGNFDKTLQEASIKHIWTYPYTPKMNAT
                     CERFNRTLREQFIEFNELLLFEDLNLFNQRMAEYLVLYNSKRPHKSLELMTPVDYILR
                     E"
     gene            1079..1240
                     /locus_tag="OHO85_004985"
     CDS             1079..1240
                     /locus_tag="OHO85_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCQ2674196.1"
                     /translation="MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTA
                     SLAYESSGK"
     gene            complement(1478..1862)
                     /locus_tag="OHO85_004986"
                     /pseudo
     CDS             complement(1478..1862)
                     /locus_tag="OHO85_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          436 a          524 c          523 g          516 t
ORIGIN      
        1 ctctcacgta aaatatagtc cactggcgtc atcagttcga gtgatttatg tggccttttg
       61 ctgttataca gcaccagata ttcagccatt ctttgattaa acaaattcag gtcctcaaaa
      121 agcaacaatt cattgaattc aataaattgt tctctaagtg tccggttaaa tcgctcacag
      181 gtcgcattca ttttcggtgt gtacggatag gtccagatgt gtttaatcga ggcttcctgt
      241 agcgttttat caaagttacc gaggaactcc ttaccgttgt cagtgacaac ttgtctgata
      301 gcgacaggaa agagctttgt ggccttgctg aagaaatggc tggtaatatc gctgttgagt
      361 gaagggaccg ccagggccag cgcatagtcg ctgtgctcgt cgatcatggt aatgatatag
      421 cggcgtaggt cccccatcct gagttcaatc gcgtccatcc ctatgagttc gcctgttttt
      481 accgggcggt attgttttgg tcttctgggc ttcactgagc gtttttttat caaccgggct
      541 ttgcccctgg cgctgaggcg tacggggatc atccgcattt tatcgtgagc accagcaatg
      601 attcttccaa tggttgacgt gctgggacag gtaaaatgcc gcgcttcaca ccatggtttc
      661 agcctgacaa aaatctgctc tttgccgaga ttgggtaact cagttctcag acgcctgatc
      721 tccttgagta catcaggatg ccagtgcctt gaacggcgaa ccagaggggc tttgcttctt
      781 ggaattagtg cttctggacc accggtgcgc agtaaccggc gccaccagta gagtgttcgc
      841 gttgatacgt caaaagcatc agctgcggca cggatcccgt gcttatccca gaagttcaga
      901 gccttcattc ttaacttcgc aatctcgggc ataagagagt gtttcatcgc ataagcagtt
      961 gctcggtaat acccaaaata gccgacaccg atatgctgca tcaacatctg gcaatccctc
     1021 ctttagtgtt ctgaggcaat tgcaatatct gtgtgaactt acacaggagg agtaaaccat
     1081 gaaactaccg ggaaacgccc ttatctggtg cgtattaatc gtgtgctgca cgttgttaat
     1141 attcacactc ctgacccgga atcgtctgtg cgaagtccgg ctgaaggacg gatacaggga
     1201 ggttacggca agtttggctt acgaatccag cggtaagtag caacctggag gcgggcgcag
     1261 gcccgccttt tcagggctga tgctggtctg actgcactga agcgccttta taaaggggct
     1321 gctggttcgc cggtggcccc tttctccttg ctggcattgt gcgggcatga acaaccagac
     1381 tgccaggaag gccgtttccc tccgggaacg gggatgaacg tgcgcctgcg gcgcccgttc
     1441 ttttcccccg ccttctctgt ttatgacctg tcagaaatca catcaccttc cctttgatta
     1501 ttctcttttt cctcctgttc tgactctgat tactgctgcc ctgattattg tctgttttgt
     1561 tgttctcctg cttatgcctt ttccgggcgt ccccttggcg gggcgggctg tcgtgaacgg
     1621 agccagcgaa gctgtctccg ccctggcggg cctccatccc tgacgcgaaa ccccgggctt
     1681 gcagcaaccg ttccggtcgc gccgcctctg tcgcgccgtg gcctgcgctc ccggcatccc
     1741 ggcaaaaggc cgccgggatg taaggggcaa cgccgtttcg caaaccgcgc ttcacgtcgt
     1801 tgcggcgggt cccgaaactc aaaagatgat gggggctgaa aaccagagag aggagaaagc
     1861 aggatgagta cccgaaatat ccacgttaac accgcgtcgt acacgcttct tgttgccggg
     1921 aagaaaaaaa caccggggaa gagagggacg tcctggaatt cagcagcctg actgagctga
     1981 aaaaatatcg caaaagcca
//
LOCUS       DAJOWB010000303         1987 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJOWB010000303 DAJOWB010000000
VERSION     DAJOWB010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.309"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1288..1890
                     /gene="fimB"
                     /locus_tag="OHO85_004987"
     CDS             1288..1890
                     /gene="fimB"
                     /locus_tag="OHO85_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HCQ2674197.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          604 a          349 c          411 g          623 t
ORIGIN      
        1 tatttaaata aacagagatc cagattaaat acctgagtat aaaatctctt ctgatgttta
       61 attgatttga atgttcgtaa gctatatcac ttactcaatc cattttaccc agagtcataa
      121 aatagaatgt ctgttgatct cattttcata ttcctttcag actgaagtgc gttttcatca
      181 cccgatgaga aagaactgaa gtgatgtttg ccatagattg cgagatgccc tgtctcattg
      241 actatcttgg tgcaccttta tacctgttat accagatcaa aaatcacgca atccatacaa
      301 caaaaccaga tttgcaattc gtgtcacaaa atatgtcgat ctttttctaa gaggaagatg
      361 ccatgtgaag ccagacgaac acttgcggtg gtcttcaaaa actaaagatc ttagtttaac
      421 tatttgtttt ataaataatt tgtttagagt ctaaacaagg ggagctttgc aagctaactc
      481 agtgagcttg gtgaaaatca gtgtttaccc gccatcaggc tgagcataat tctcatcatg
      541 aaatatgttt cctggtttgt ggcttgtaac tggtcacttc tgaagtcgat ctggagaggc
      601 ttgttgatgt tggtgttttc aggatgatgt ttcacttagt ttgtttgccg tatcgcccgg
      661 cgaatggctg tgattgagga aggtttaagt cgtagtgacc aaagctatat ttaccaacga
      721 atgtagatga aaaaatcatc tcctgcgttc ccccatatct ccaggataaa aaggaatgta
      781 acaatctcat ggcgtaagct gacgaatcag caggaataat cgctagggac ctaagaatta
      841 gcatgataat agccactaag aaattactgc gctccatgaa atagccattt tgtggcaaat
      901 ggagttgact aataatgtca tatgtgagac ggctagttga acgaatatta aattttgctg
      961 aattttttat gttgatttta cttgttacag aacatatcac atgatatata gataagatta
     1021 gttgcattaa tgatgagggt tattattaga ttcgtatccg attgataaat atataaaggt
     1081 acatagcatg caagagcatg gcgtttgtat ggcaacgtta ttataattaa cagttgctac
     1141 tccatttaag ttcactcaga agaactggtc cacttacgtt agttattaag caaacgttcg
     1201 cttttataaa cataatcagg ataaaaatgt tggattattg ctaacccagc acagctagtg
     1261 cgcgtctgta attataaggg aaaaacgatg aagaataagg ctgataacaa aaaaaggaac
     1321 ttcctgaccc atagtgaaat cgaatcactc cttaaagcag caaataccgg gcctcatgca
     1381 gcacgtaatt attgtctgac tttgctttgt tttattcatg gtttccgggc gagtgaaatt
     1441 tgtcgattga ggatttcgga tattgatctt aaggcaaagt gtatatatat ccatcgatta
     1501 aaaaaaggct tttcaacaac gcacccgcta ttgaataaag aagttcaggc tttaaaaaac
     1561 tggttgagta tccgtacttc gtacccgcat gctgagagcg agtgggtatt tttatcacgt
     1621 aaggggaatc cgctttctcg gcaacagttt taccatatta tctcgacttc cggtggtaat
     1681 gccgggttgt cactggagat tcatccgcac atgttacgcc attcgtgtgg ttttgctttg
     1741 gcgaatatgg gaatagatac gcgacttatc caggattatc ttgggcatcg caatattcgt
     1801 catactgtct ggtataccgc cagcaatgca gggcgttttt acggcatctg ggatagagcc
     1861 agaggacgac agcgtcacgc tgttttatag agatagttga gataccaggg atggtgttaa
     1921 aaactacatt attcttacga tatatcaaaa atgattaacc atttattaat tgatgttatt
     1981 gtaagtg
//
LOCUS       DAJOWB010000304         1981 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJOWB010000304 DAJOWB010000000
VERSION     DAJOWB010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.310"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            218..943
                     /locus_tag="OHO85_004988"
     CDS             218..943
                     /locus_tag="OHO85_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674198.1"
                     /translation="MKGEVKERGMIFNDEMVRAILEGRKTQTRRIMKNQPAEVGPEAP
                     VMVREIGAGFQWYGADGVSSVFNCPFGIVGDRIWVRETWAILGNEDGCSVDWNDNLCR
                     GDEKNAARIYRASCEQKPGDYGLWSIPDDADWKPHTVNEKFDGGWRPSIHMPRWASRI
                     LLEITNVRVERLNDISECDARDEGVPPAGSLLPDHPGTFLTPKGDFAMAKVAFQRLWE
                     SIYGEESWSANPWVWVIEFERIQ"
     gene            983..1174
                     /locus_tag="OHO85_004989"
     CDS             983..1174
                     /locus_tag="OHO85_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HCQ2674199.1"
                     /translation="MNKASPAELRASLEMAHSLAQIGVRFVPIPVETDEEFHRLAASA
                     AQKLEIMAAKVEKAEGATK"
     gene            1171..1386
                     /locus_tag="OHO85_004990"
     CDS             1171..1386
                     /locus_tag="OHO85_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674200.1"
                     /translation="MSKPTDEEIVRVLEEHGRCMTYVVTNWLRDKYRTLKTAYVLRRL
                     KKLEFDGKVKRVNSSYIRQICWEASSE"
     gene            1379..>1981
                     /locus_tag="OHO85_004991"
     CDS             1379..>1981
                     /locus_tag="OHO85_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674201.1"
                     /translation="MSNRFYMMCLRETVGNNASFHCHNGNGYSSDIDRAHVYTLEEAQ
                     KAWNCGRDIDQPVCADSVDAMAVWHVDCQYIPTESLIESDCTAYVAYKKGSWNGNDVY
                     WLQHGGLPTDDFSKATIFSVANKNEPGIVWLPFSIADAAKRRTFNINKFNRRTMVQGA
                     GLVMPDWLEEQNRRKKSRSGKVRWNCPHCGKITWQYNPYDF"
BASE COUNT          561 a          399 c          551 g          470 t
ORIGIN      
        1 acgcgcttct ccatcaacaa taattgctct aataaatctt gatttttaaa acagagaaag
       61 tgaaaataaa aacatgccgt aaggcgcggc atgtatccaa tcaatcacag gagctgaaaa
      121 tatgaacacg gcattcaaaa tcattatggc cgcgatctat ttctggctgt tctctatcac
      181 ttttggcggc atcgtcgcgc atgggtaagg gggatgcatg aaaggcgaag tgaaagagcg
      241 cggcatgatt ttcaacgatg agatggtccg agccatcctc gaaggaagga aaacgcagac
      301 gcggcggata atgaaaaatc agcctgcgga agttggtcca gaagcaccag tgatggttag
      361 ggaaattggt gcaggttttc agtggtacgg ggctgatggt gtaagcagtg ttttcaattg
      421 cccctttggt atcgtcggcg atcgaatttg ggttcgtgaa acatgggcga tattaggcaa
      481 tgaagatggt tgcagtgtgg actggaacga caacctttgt cgtggcgatg agaagaacgc
      541 agcaaggatt tatcgggcca gttgtgagca aaagcctggt gattacggtt tatggtcgat
      601 acccgatgat gccgactgga aacctcacac tgtgaatgaa aagtttgatg gtgggtggcg
      661 tccatcaatt cacatgccgc gatgggcatc acgcatcctg ttggaaatta ccaacgtgcg
      721 cgttgagcgg ttgaacgata tcagcgaatg cgatgcaagg gatgagggcg ttccgcctgc
      781 tggaagtttg cttcctgatc acccgggaac attccttact cccaaggggg atttcgcaat
      841 ggccaaggtt gcgttccagc gcctgtggga atccatctac ggcgaagaaa gctggagtgc
      901 taatccctgg gtttgggtaa tcgagtttga gcgtattcag tagggcgagc gtatgcaatc
      961 agttatttgt gaggaagtcg gcctgaataa agcatcccca gcagagttaa gagcaagtct
     1021 cgaaatggcg catagccttg ctcaaattgg tgtcaggttt gtaccaattc cagttgaaac
     1081 agatgaagaa tttcacaggt tagcggcatc ggcagcacaa aagctggaaa tcatggcagc
     1141 gaaagtagag aaagctgaag gagcgacaaa atgagcaagc caaccgacga agaaatagtt
     1201 cgggtgttgg aagaacacgg gcgctgtatg acttacgtcg tgaccaactg gcttagggat
     1261 aaatatcgca cactcaaaac ggcatacgtt ttgcgtcgtc taaagaaact ggagttcgac
     1321 gggaaagtaa agcgcgttaa cagttcttat ataagacaaa tttgctggga ggcatccagt
     1381 gagtaaccgt ttttacatga tgtgcttgcg tgaaactgtg ggtaataacg cctcattcca
     1441 ttgccataac ggcaatggtt acagttctga tatcgatcgc gctcatgttt acacgctgga
     1501 agaagcccaa aaagcctgga attgtgggcg agatatcgat cagcctgttt gtgccgatag
     1561 tgtggatgca atggctgtgt ggcacgttga ttgccagtac atccctacag aaagcctgat
     1621 tgagtcagat tgcactgcgt atgtggccta caaaaaaggt agctggaacg gcaacgatgt
     1681 ttactggctt caacacggtg gattgccaac agatgacttc agtaaagcga ccatctttag
     1741 cgtcgccaac aaaaacgaac caggaatagt ttggttgcca ttttccattg ctgatgcagc
     1801 aaagcgccgg acgttcaata tcaataaatt taaccgcaga acaatggttc agggcgcagg
     1861 tttggtcatg cctgactggt tggaagagca gaacagaaga aagaagtcgc gaagcgggaa
     1921 ggtgcgttgg aattgtccgc attgcggaaa aataacctgg cagtacaacc catatgattt
     1981 t
//
LOCUS       DAJOWB010000305         1977 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJOWB010000305 DAJOWB010000000
VERSION     DAJOWB010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.311"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            163..945
                     /locus_tag="OHO85_004992"
                     /pseudo
     CDS             163..945
                     /locus_tag="OHO85_004992"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            1120..1494
                     /locus_tag="OHO85_004993"
     CDS             1120..1494
                     /locus_tag="OHO85_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674202.1"
                     /translation="MIPHRENHLVLDIANSESETELQGNRQIIAPYRGAVSYVQFTTD
                     QRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRVDEIPTGIKVALNDE
                     QNLFCTITFQHVIDENKTYICQ"
     gene            1507..1977
                     /locus_tag="OHO85_004994"
                     /pseudo
     CDS             1507..1977
                     /locus_tag="OHO85_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
BASE COUNT          644 a          386 c          365 g          582 t
ORIGIN      
        1 agcatactct gaaacatcct actctctcac tctaataatg gcaccttgct cttcttataa
       61 agaagggaat aattaagaat attaaacttt catcaacttc acatcaagaa attaaaaatt
      121 ttaaacatta atctaaataa taacaacctg atcacataat cgggtacgat tttatccgat
      181 aactataatg ctataactct gggaaatggt tggaatacac cgctgggtgc catctctttt
      241 tacgctgccc gatcgagtag taagttaaat aatgatacca gacacgaagg tacgagctat
      301 caggtcgcct ataataaata tctagtacaa acagccactc gatttagcgt ggccgcctgg
      361 cgttatgctt cgcaggatta caggacattc agcgaccatc tttacgaaaa tgataaacac
      421 tatcatcaga gtgattatga tgatttttat gatattggca gaaaaaatag cctttctgct
      481 aatattatgc aacctttatc caataatctg ggaaatgtat cattaagtgc gctttggcgg
      541 aattactggg ggcgaagtgg aaatgctaaa gattaccaat tcagttactc caataactgg
      601 caacacatta gttatacttt ctctgccagc caatcttatg atgaaaataa taaagaagag
      661 gagcgtttta atctgtttat ctccattcct ttctactggg gggatgatat tgccaaaaca
      721 cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta ttcctccaac
      781 aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg tatatatgtt
      841 aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg gagaactccc
      901 atcgccataa tagatggcag ctatagtcat tctaaaaacg cctagcaaag tggtggaagt
      961 attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca gttatccgat
     1021 acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg acaaaaatac
     1081 aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca tcgtgaaaac
     1141 catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg caatcgtcaa
     1201 attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga ccaacgtaag
     1261 ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg ctatgacgta
     1321 ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct ttttattcgc
     1381 gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa cttattctgt
     1441 actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca gtaaaaggag
     1501 tttactatga agtggctttt attaatcacc ttgagtctat attcttttat tgtgcaaagt
     1561 gctccctgtg cattgaccaa tgtcggtgaa caacgtggaa catatatact caagccactg
     1621 agtatgaaag gtaatctaac cgccgaaaaa ttgaaaacac cggaaatatc attgagtgta
     1681 cagacttgca agagacaacc aacgccatta aagttcattt tttaacacag aacgctttaa
     1741 ggtttaaagt cggaagtcgt cattatatta ttaatttccc ctttgaatct gacggtaaaa
     1801 aaatagagct tccagggaag tcatcttata atttagaaga aatgctatct gcgataaatt
     1861 tcaccattcc atattctatc gcatctgtat cagatagttc taacaccgca gacatttttc
     1921 ctggaactcc ttttcctttg ttgttcgaaa ttgaagtgag ttcatgtaca gggaata
//
LOCUS       DAJOWB010000306         1969 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJOWB010000306 DAJOWB010000000
VERSION     DAJOWB010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.312"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..313
                     /locus_tag="OHO85_004995"
     CDS             <1..313
                     /locus_tag="OHO85_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCQ2674203.1"
                     /translation="QPFDRQIVIPAVAFSGAKHGRGDNETYSSCRLVVKKNGSVIYDR
                     STTDVTAIYTGIMDMPAGSGGITLEFSVSASNGHYYPTASISDLLVVVMKKATAGISI
                     S"
     gene            381..980
                     /locus_tag="OHO85_004996"
     CDS             381..980
                     /locus_tag="OHO85_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCQ2674204.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEQQATLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYIRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTNSFIVGVGYKF"
     gene            1045..>1969
                     /locus_tag="OHO85_004997"
     CDS             1045..>1969
                     /locus_tag="OHO85_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCQ2674205.1"
                     /translation="MAVRISGVLKDGAGKPIQNCTIQLKARRNSTTVVVNTVASENPD
                     EAGRYTMDVEYGQYSVSLLVEGFPPSHAGIITVYEDSKPGTLNDFLGAMTEDDVRPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSAGDAGTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSASAAKTSETNAAASQKSA
                     ATSASAATTKASEAATSARDAAASKEAAKSSETNASSSASSAASSATATANSAKAAKT
                     SETNAKSSETAAGQSASAAAGSKTAAASSASA"
BASE COUNT          478 a          507 c          584 g          400 t
ORIGIN      
        1 tcagccgttt gaccggcaga ttgttattcc ggcggtggca ttcagcggcg ctaaacatgg
       61 gcggggggat aacgagactt attcatcctg tcgtcttgtt gtaaaaaaga atggttctgt
      121 aatttatgac agatcgacca ccgatgtcac agccatttac acaggtatca tggatatgcc
      181 tgccggttct ggaggaataa ctctggagtt ttcagtttct gcgtcaaacg ggcattatta
      241 tcccacagca agtatcagcg atttgctggt tgtggtgatg aagaaagcca ctgcaggcat
      301 cagtatcagc tgaattttat aacccatata cgggcgccag aaatggcgcc ttttttattg
      361 cagaaaagcg agaggtaatt atgcgtaaag tttgtgcagc cattttgtcc gcagccatct
      421 gtctggccgt atccggtgcg cctgcatggg cgtctgagca gcaggccaca ctgagcgcag
      481 ggtatcttca tgcccgtacg aacgctcccg gcagcgataa tctgaacggg attaacgtga
      541 aataccgtta tgagtttacg gacacgctgg ggctgattac gtcattcagt tatgccaacg
      601 ctgaagatga gcaaaaaacg cattacagcg atacccgctg gcatgaggat tccgtgcgta
      661 atcgctggtt cagcgtgatg gcggggccgt ctgtgcgcgt gaatgaatgg ttcagcgcgt
      721 atgcgatggc gggtatggct tacagccgtg tgtcgacttt ctccggggat tatatccgcg
      781 taactgacaa caaggggaaa acgcacgatg tgctgaccgg aagtgatgac ggtcgccaca
      841 gcaacacgtc tctggcgtgg ggagctggcg tgcagtttaa cccgaccgaa tccgtggccg
      901 ttgacgtcgc ttatgaaggc tccggcagcg gtgactggcg taccaacagt ttcatcgttg
      961 gtgtcggtta taaattctga ttagccaggt aacacagtgt tatgacagcc cgccggttca
     1021 ggcgggcttt tttgtggggg gaatatggca gtacggattt caggtgtact gaaagatggc
     1081 gcaggtaagc cgatacaaaa ctgcaccatt cagctaaagg ccaggcgcaa cagcaccacg
     1141 gtggtggtga acacagtggc ctcagaaaac ccggatgaag ccgggcgtta cacaatggac
     1201 gtcgagtatg gtcagtacag cgtcagtctg ttggtggagg gattcccgcc gtcacacgcc
     1261 gggattatca ccgtatatga ggactcaaag ccgggcacac tgaatgattt cctcggtgca
     1321 atgacggagg atgatgtccg gccagaggca ctgcgccgtt ttgaactgat ggtggaagag
     1381 gtggcgcgta acgcgtccgc agtggcacag aacacggcag ccgcgaagaa gtcagccggc
     1441 gatgccggca catctgcccg tgaggcggca acccatgcga ctgatgctgc aggctcagca
     1501 cgcgcagcca gcacgtcagc cggacaggcc gcttcgtcgg ctcagtcagc gtcttccagc
     1561 gcaggaacgg catcaacaaa ggccactgaa gcatcgaaaa gtgctgctgc tgcagagtcc
     1621 tcaaaaagcg cggcagctac cagtgccagt gccgcgaaaa cgtcagaaac gaatgcggca
     1681 gcgtcacaaa aatctgcagc cacttctgca tccgccgcga ccacgaaggc gtcagaagct
     1741 gccacctcag cccgggatgc ggcggcctca aaagaggcag cgaaatcatc agaaacgaac
     1801 gcatcatcaa gcgccagtag tgccgcttcc tcggcaacgg cgacagcaaa ttctgcgaag
     1861 gcggccaaaa cgtccgagac gaacgctaag tcttctgaaa cggcagcggg acagagtgcc
     1921 tcagctgcgg caggctcaaa aacagcggct gcgtcgtctg ccagcgccg
//
LOCUS       DAJOWB010000307         1951 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJOWB010000307 DAJOWB010000000
VERSION     DAJOWB010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.313"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            86..1108
                     /gene="istA"
                     /locus_tag="OHO85_004998"
     CDS             86..1108
                     /gene="istA"
                     /locus_tag="OHO85_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HCQ2674206.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRGFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGENLVNFDKHPLHHPLSIYDS
                     FCRGVA"
     gene            1108..1887
                     /gene="istB"
                     /locus_tag="OHO85_004999"
     CDS             1108..1887
                     /gene="istB"
                     /locus_tag="OHO85_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCQ2674207.1"
                     /translation="MMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFLE
                     HLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERN
                     ENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQR
                     GVMAPRLLIIDETGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDA
                     ALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT          507 a          506 c          501 g          437 t
ORIGIN      
        1 gtcacgaacg gtgcaatagt gatccacacc caacgcctga aatcagatcc agggggtaat
       61 ctgctctcct gattcaggag agcttatggt cacttttgag acagttatgg aaattaaaat
      121 cctgcacaag cagggaatga gtagccgggc gattgccaga gaactgggga tctcccgcaa
      181 tacggttaaa cgttatttgc aggcaaaatc tgagccgcca aaatatacgc cgcgacctgc
      241 tgttgcttca ctcctggatg aataccggga ttatattcgt caacgcatcg ccgatgctca
      301 tccttacaaa atcccggcaa cggtaatcgc tcgcgagatc agagaccagg gatatcgtgg
      361 cggaatgacc attctcaggg gattcattcg ttctctctcg gttcctcagg agcaggagcc
      421 tgccgttcgg ttcgaaactg aacccggacg acagatgcag gttgactggg gcactatgcg
      481 taatggccgc tcaccgcttc acgtgttcgt tgctgttctc ggatacagcc gaatgttgta
      541 catcgaattc actgacaata tgcgttatga cacgctggag acctgccatc gtaatgcgtt
      601 ccgcttcttt ggtggtgtgc cgcgcgaagt gttgtatgac aatatgaaaa ctgtggttct
      661 gcaacgtgac gcatatcaga ccggtcagca ccggttccat ccttcgctgt ggcagttcgg
      721 caaggagatg ggcttctctc cccgactgtg tcgccccttc agggcacaga ctaaaggtaa
      781 ggtggaacgg atggtgcagt acacccgtaa cagtttttac atcccactaa tgactcgcct
      841 gcgcccgatg gggatcactg tcgatgttga aacagccaac cgccacggtc tgcgctggct
      901 gcacgatgtc gctaaccaac gaaagcatga aacaatccag gcccgtccct gcgatcgctg
      961 gctcgaagag cagcagtcca tgctggcact gcctccggag aaaaaagagt atgacgtgca
     1021 tcctggtgaa aatctggtga acttcgacaa acaccccctg catcatccac tctccatcta
     1081 cgactcattc tgcagaggag tggcgtgatg atggaactgc aacatcaacg actgatggcg
     1141 ctcgccgggc agttgcaact ggaaagcctt ataagcgctg cgcctgcgct gtcacaacag
     1201 gcagtagacc aggaatggag ttatatggac ttcctggagc atctgcttca tgaagaaaaa
     1261 ctggcacgtc atcaacgtaa acaggcgatg tatacccgaa tggcagcctt cccggcggtg
     1321 aaaacgttcg aagagtatga cttcacattc gccaccggag caccgcagaa gcaactccag
     1381 tcgttacgct cactcagctt catagaacgt aatgaaaata tcgtattact ggggccatca
     1441 ggtgtgggga aaacccatct ggcaatagcg atgggctatg aagccgtccg tgcaggtatc
     1501 aaagttcgct tcacaacagc agcagatctg ttacttcagt tatctacggc acaacgtcag
     1561 ggccgttata aaacgacgct tcagcgtgga gtaatggccc cccgcctgct catcattgat
     1621 gaaacaggct atctgccgtt cagtcaggaa gaagcaaaac tgttcttcca ggtcattgct
     1681 aaacgttacg aaaagagcgc aatgatcctg acatccaatc tgccgttcgg gcagtgggat
     1741 caaacgttcg ccggtgatgc agcactgacc tcagcgatgc tggaccgtat cttacaccac
     1801 tcacatgtcg ttcaaatcaa aggagaaagc tatcgactca gacagaaacg aaaagccggg
     1861 gttatagcag aagctaatcc tgagtaaaac ggtggatcaa tattgggccg ttggtggaga
     1921 tataagtgga tcacttttca tccgtcgttg a
//
LOCUS       DAJOWB010000308         1948 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJOWB010000308 DAJOWB010000000
VERSION     DAJOWB010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.314"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            68..1714
                     /gene="mqo"
                     /locus_tag="OHO85_005000"
     CDS             68..1714
                     /gene="mqo"
                     /locus_tag="OHO85_005000"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCQ2674208.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPKPVQKEVADIAL"
BASE COUNT          468 a          479 c          581 g          420 t
ORIGIN      
        1 agtgttaccg ttgatgccgc gcaaatctca cctgcaataa agcgactaaa agtaaggcat
       61 taacaagatg aaaaaagtga ctgccatgct cttctcgatg gccgtggggc ttaatgccgt
      121 ttcgatggcg gcaaaagcga aagcgtctga ggagcaggaa actgatgtac tgttgattgg
      181 cggcggcatt atgagcgcca cgttggggac ctatttacgc gagctggagc ctgaatggtc
      241 gatgaccatg gtggagcgcc tggagggtgt cgcgcaggag agttcgaacg gctggaataa
      301 cgccggaacc gggcattctg cactgatgga actgaactac accccgcaaa acgccgatgg
      361 cagcatcagt attgaaaaag cagtcgccat taacgaagca tttcagattt cccgccagtt
      421 ctgggcgcac caggttgagc gcggcgtgct gcgtactccg cgttcattta tcaataccgt
      481 tccgcatatg agctttgtct ggggcgagga taacgttaat ttcctgcgcg cccgttacgc
      541 cgcgttgcaa caaagctcgc tgtttcgcgg tatgcgttac tctgaagatc acgcgcagat
      601 caaagagtgg gcaccgttag tgatggaagg gcgcgatccg caacagaaag tggcagccac
      661 gcgtacggaa attggtaccg atgtgaacta cggcgagatc acccgccagt taattgcttc
      721 cttgcagaag aaatctaact tctcgctgca actcagcagc gaagtccgcg ccctaaagcg
      781 taatgacgat aacacctgga ccgttaccgt tgccgatctg aaaaatggca ctgcacagaa
      841 cattcgtgcc aaatttgtct ttatcggcgc gggcggtgcg gcgctgaagc tgttacagga
      901 atcggggatt ccggaagcga aagactacgc cggtttcccg gtgggcggac agttccttgt
      961 ttcggaaaac ccggacgtgg ttaatcacca tctggcgaag gtttacggta aagcatccgt
     1021 tggcgcacca ccgatgtcgg ttccgcatat cgatacccgc gttctggacg gtaaacgcgt
     1081 agtgctgttt gggccatttg ccaccttctc aaccaaattc ctcaaaaacg gttcattgtg
     1141 ggatctgatg agttccacca ccacctctaa cgtgatgccg atgatgcacg tcgggctgga
     1201 taatttcgat ctggtgaaat atctggtgag tcaggtgatg ttgagtgaag aggatcgttt
     1261 tgaagcgttg aaagagtact atccgcaggc gaaaaaagag gactggcgtt tgtggcaagc
     1321 ggggcagcgc gtgcagatta tcaagcgtga tgccgagaaa ggtggcgtac tgcgtctggg
     1381 tactgaagtc gtcagtgacc agcaagggac cattgccgcg ctcctggggg catcgccagg
     1441 ggcgtcaacc gccgcgccga ttatgttgga tctgctggaa aaagtatttg gcgatcgcgt
     1501 ttccagcccg caatggcagg ctacgttgaa agcgatcgtt ccgtcttatg gacgcaagct
     1561 gaacggtgat gtagcggcaa cagaacgcga gttgcagtac accagcgaag tgctggggct
     1621 gaaatacgac aagccgcagg cagcagatag tacgccgaaa ccgcagttga aaccgaaacc
     1681 cgttcaaaaa gaagtggcgg atattgcgtt gtaatgatgt gccacatccg gcatggtatg
     1741 ccggggatgt ggcgtatgct gataagacgc gccagcgtct tatcaggcaa ccggctcggg
     1801 cgttagatgt cggatgcggc gtaaccgcct tatccaatct acggggtgca gcacttcaca
     1861 gcggtgtagg cctgataaga cgcgccagcg tcgcatcagg caaccggctt gggcgttaga
     1921 tgtcggatgc ggcgtaaccg ccttatcc
//
LOCUS       DAJOWB010000309         1924 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJOWB010000309 DAJOWB010000000
VERSION     DAJOWB010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.315"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            847..1924
                     /locus_tag="OHO85_005001"
                     /pseudo
     CDS             847..1924
                     /locus_tag="OHO85_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660973.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
BASE COUNT          511 a          389 c          524 g          500 t
ORIGIN      
        1 cgacgccgac gcgtgtttgt tgtcgcaagt gctcgaacag acctcgatcc cgcaacggta
       61 ctttttgagt tcgaaggcgt gcgccgggat attgcgccga gcagaggcga ggggaaggaa
      121 gttgccggaa atgttggaaa tggcattaaa agcgggagcc attgggataa ccctgtaaat
      181 ccgcacccga cacttaacca gtcacacaat actggcggga tcggcgcaag taaccaggag
      241 atttttgcgc agcgcggaag tgggcttgtt ggtgcttatc gaatggttgc atttggcgaa
      301 tatgctgatg atgagactgc ttctactgtc aaggccagag attttaaaga tgcaaccgat
      361 ttagcagttt tcagtagtac tggtgctggt atttggcgtg aaggtgcagg cactcttcgt
      421 gccagatcac aagaaagtca cgaacatctg gcagtgatgg ctattcatgg aacacaagat
      481 cctgatgtta attgtgaatt ggcacatact attggtcgta accatggtca agaaaatgca
      541 gtcgtagctt tttcctgcaa agattatgga caagatgttt ctattgaatg ttctccgact
      601 ttacgatctg gtaatgctgt taatagcaat ccaaatgcag gatgcccacc agctgttgcg
      661 tattcgctac aacatgcaca aattgggcga aaagatgatg ctggaccgca gggtaaaggg
      721 tggcaaaaag agataagttt tactcttgat tcccgcgcta cagctgacgc cgttgcgtta
      781 tcttttgggg ggcagaaaag tattgaaaaa ggcaatttag gtgagaatgg ttacttacct
      841 catatgatga gtgttcgccg tcttacccct gttgaatgtg aaaggctgca aggttttcct
      901 gatgggcata cgttgatccc tacggaaaag cgtaaaaaag tttcttcaga tgaactggca
      961 taccttcgca ataactatcc tgatttaagt gaagaagagg ccgcgatgct tgcagctgac
     1021 ggaccgcgtt acaaagcgat cggcaatagt atggcgatac cagtaatgcg ctggattggc
     1081 gatcggattg ccaaggctgc atgtcggcag aaggaaggga gtgaaacaaa agagcgaaaa
     1141 gttaaaccag cggcagaatt cgaacggtcc atattcaaat gggctggtgg aaaatttggt
     1201 gttctggaac aaatctttcg ctatttgcca gaagggaagc gcctgattga acctttcgtt
     1261 ggtggcggag ctgtcttcat gaatgccgga taccaggaaa atctgctaaa tgatgtgaat
     1321 gctgacctga ttaactttta caagactttg caacgtgagg aacgttcact tatcactctg
     1381 gcgcatcggt ttttccagga ctacaacaca caggaaggat acctggcagt acggagtgcg
     1441 tttaacaaac aagtctatga tgatttacat cgcgcagcgg cgtttttgtt cctgaaccga
     1501 cattgtttta acggattgac gcgttacaac caggccggtg agttcaatgt cggttatggg
     1561 aagtataaaa ctccgtattt cccattacag gagatggaag ccttcctcgg tgcggaaggg
     1621 cggtctgagt ttgtatgcgg tgattttgca gcggtgattg aagctgccgg agaaggtgat
     1681 gtcatctttt gcgatccgcc gtatgaaccg cttccaaata cagagggatt cacgaactat
     1741 tccggtcatg actttaagtt tgaagagcaa aaacgcctgg tgtctctgtt gacggatgct
     1801 catcgtcgag gtgcaaaggt tctcattact aacagtggcg cgccaaacat cagagagctt
     1861 tatcatgaca gtggcttcag agtggaacat ctttttgcca gacgttctgt gtcttgtaag
     1921 gggg
//
LOCUS       DAJOWB010000310         1870 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJOWB010000310 DAJOWB010000000
VERSION     DAJOWB010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.316"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..835
                     /locus_tag="OHO85_005002"
     CDS             <1..835
                     /locus_tag="OHO85_005002"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCQ2674209.1"
                     /translation="ATFCLLIAKNVIKDKKIIVHWHSDIIKQKKMKIFFAPFQHYMLK
                     RADLIITTSEPYRLSSECLRQFLPKTKTVPIGVVSLVNKVDFQHVKLIKKKYKNKFII
                     FSLGRHVYYKGFQYLIESAQYISDNCVVLIGGHGPMTEQLNNLLIEKNLQDKVEILGN
                     IPVDSLASYFYVADVFCIPSIERSEAFGIVQIEAMSLGKPVISTDITGSGVPWVNKNM
                     CSGLVVNVKSSQALSDAISKLHTDYELYRKLSKGAIYRYEKHFSKNVMIDSFLNIYRS
                     I"
     gene            837..>1870
                     /locus_tag="OHO85_005003"
     CDS             837..>1870
                     /locus_tag="OHO85_005003"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HCQ2674210.1"
                     /translation="MLLPVIMAGGVGSRLWPMSRACYPKQFLSFSQKNSMLQDTILRL
                     KGIEHFPVSLICNEEHRFLVAEQLRELNLPCTNIILEPQGRNTAPAVALAALNALEIN
                     SESILLVLPADHFIKDSEVFANYIEKALPVAENGKLVTFGISPTRPETGYGYIKKGVE
                     FDASGIAYCVEKFVEKPNECLAKKYLESGKYYWNSGIFMFKASCYMSALKEFRPDIVL
                     TVENVYRSAKSEGAFIYFDEKDFSACPSESIDYAVMEKTKEAVMIPMDVGWNDMGSWG
                     ALWDINQKDENGNVILGDALIEDSHNCYINSSDRLISAIGISNLAIVDTKDAILITHR
                     GKTQNVKNIVS"
BASE COUNT          600 a          265 c          378 g          627 t
ORIGIN      
        1 ggcaacattt tgtcttctta ttgcaaaaaa tgtcattaaa gataagaaga ttattgttca
       61 ttggcatagt gatataataa agcaaaaaaa aatgaagatt ttttttgctc cgtttcaaca
      121 ctatatgctt aaacgagctg atttaataat aacaacaagt gaaccatatc gcttaagttc
      181 tgagtgcctc agacaatttc ttccgaaaac aaaaactgtt cctataggtg tcgtcagtct
      241 cgttaataag gttgattttc aacatgttaa attaataaag aaaaaatata aaaataaatt
      301 cattatcttt tctttaggta ggcatgttta ttataaaggt tttcaatatt taattgagtc
      361 agcacaatac atatctgata attgtgtcgt gttaattggc ggtcatggac ctatgacgga
      421 gcaattaaat aatttattga ttgaaaaaaa cttacaagac aaagtggaaa ttcttgggaa
      481 tatacccgtt gatagtttgg cttcttattt ctatgtagca gatgtatttt gtattccatc
      541 aatagaaaga agtgaagcat ttggtatcgt tcagattgag gcgatgtccc ttggtaaacc
      601 agttatatcg acagatataa ctggttcagg cgttccttgg gttaataaaa atatgtgttc
      661 tggacttgta gtaaatgtta agagttctca ggcattatca gatgctatta gtaaattaca
      721 tacggattac gagttgtatc ggaaattatc aaaaggggct atttatcgtt atgaaaagca
      781 ctttagcaaa aacgttatga ttgattcttt tctaaatata tataggagta tatgatttgc
      841 ttttacctgt tataatggct ggcggtgttg gttctcgcct ttggcctatg tcaagggctt
      901 gttatccgaa acaatttctt tcttttagtc aaaaaaactc aatgttgcaa gatactattc
      961 ttagattgaa aggtatcgag cactttcctg tttctttgat atgcaatgag gagcatcgct
     1021 ttttagttgc agagcaatta cgagagttaa atttaccatg tacaaatatc attttggaac
     1081 cgcagggacg taatacagcc ccagcagtgg ccttagccgc actaaatgcc ttggaaatta
     1141 attcagaatc tatcttactt gtattgcctg cagatcattt tataaaagat agtgaagttt
     1201 tcgctaacta tattgaaaaa gctctaccag ttgcagaaaa tggaaaactt gtaactttcg
     1261 gtatttcccc taccagacct gaaactggtt atggatatat aaagaaagga gtggagtttg
     1321 atgcatcagg tatcgcttat tgtgtcgaaa aatttgttga gaagcctaat gaatgtttgg
     1381 ctaaaaaata tttagaatct gggaaatact attggaatag tggtatattc atgtttaagg
     1441 cctcttgtta tatgtctgct ttgaaggagt ttagaccgga tatagtttta acggttgaga
     1501 acgtttatcg ttctgctaaa tccgaagggg catttatata ttttgatgag aaagactttt
     1561 ctgcatgtcc tagtgagtct attgattatg cggttatgga gaaaactaaa gaggctgtga
     1621 tgattcccat ggatgttggg tggaatgaca tgggaagctg gggagcgtta tgggatatta
     1681 atcaaaagga tgagaatgga aatgtcattc ttggtgatgc tttaatagag gacagccata
     1741 attgttatat aaattcttcg gatcgattaa tttctgcaat aggtatcagt aatctggcaa
     1801 ttgtcgatac gaaagatgct attcttataa cacacagagg caagacgcaa aacgttaaaa
     1861 atatcgtatc
//
LOCUS       DAJOWB010000311         1865 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJOWB010000311 DAJOWB010000000
VERSION     DAJOWB010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.317"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..1468)
                     /locus_tag="OHO85_005004"
     CDS             complement(<1..1468)
                     /locus_tag="OHO85_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCQ2674211.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKATEALKSAAAAESSKSAAATSAGAAKTSETNAAASQQSA
                     ATSASAATTKASEAATSARDAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAV"
     gene            complement(1533..>1865)
                     /locus_tag="OHO85_005005"
     CDS             complement(1533..>1865)
                     /locus_tag="OHO85_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCQ2674212.1"
                     /translation="DSVRNRWFSVMAGPSVRVNEWFSAYAMAGVAYSRVSTFSGDYLQ
                     VTDNTGKTHDVLIGSDDGRHSNTSLAWGAGVQFNPTESVAVDVAYEGSGSGDWRTDGF
                     IVGVGYKF"
BASE COUNT          297 a          589 c          520 g          459 t
ORIGIN      
        1 ttactgcctt tggcgttgcc gccagcgttt cagacgtact gttggtcgca ctgctgagct
       61 gtactatccc ctttttcgtc gtacttgcat cctcaagcgc cacggcggat gcaatatcct
      121 ctgcccgttt tgccgctgtc tcggcgcgcg ttgccgcgga ttccgccgta cttttgctct
      181 gtgctgccgc cgtcgcactg ccagctgcct ctgtcgcctt cgtggatgcc gtcgtggcgc
      241 tgcccttcgc tgcggacgct tgtctggtcg cctcatcttt tgaagcagac gccgatgatg
      301 ccgatgacgc cgccgaactg gcggacgatg cggcagccgt ttttgaggat tctgcgctgg
      361 tttccgacgc tttcgcgttc gtctcggatg tcttcgctgc ggaagcagac cttgctgctg
      421 cgctggcctg ttcagtggct tcgccagcct tcgttgtggc tgttgaagca gacgatgcgg
      481 cgctttctgc cgattttccg gcggcggtgg cactggctga ggcctgcccg gcacttgttg
      541 acgcggcact ggcagacgac gcagccgctg tttttgagcc tgccgcagcc gaggcgctct
      601 gtcccgctgc cgtttcagaa gacctggcgt ttgtctcaga cgtctttgcc gccttcgcgg
      661 aattgcctgc cgccgttgcc gaggaagcgg cactactggc gcttgatgat gcgttcgttt
      721 ctgatgattt cgctgcctct tttgaggccg ccgcatcccg ggctgaggtg gctgcttctg
      781 acgccttcgt ggtcgcggcg gatgcagaag tggctgctga ttgttgtgac gctgccgcat
      841 tcgtttctga cgttttcgcc gcaccggcac tggtagccgc cgcgcttttt gaggactctg
      901 cagcggcagc actttttaat gcttcagtgg cctttgttga tgccgttcct gcgctggaag
      961 acgctgactg agccgacgaa gcagcctgtc cggctgacgt gctggctgcg cgtgctgagc
     1021 ctgcagcatc agtcgcatgg gttgccgcct cacgggctga tgtgctggca tcgctggctg
     1081 acttcttcgc ggctgccgtg ttctgtgcca ccgcggacgc gttacgcgcc acctcttcca
     1141 ccatcagttc aaaacggcgc agtgcctcag gacgggcatc atcctccgtc atggcaccga
     1201 gaaaatcatt cagcgtaccg ggtcgggaat cttcatacac ggtgatggtc ccggcatgtg
     1261 acggcgggaa tccttccacc aacagaataa cgctgtactg accgtactca acgtccatgc
     1321 tgtaacgccc ggcttcatcc ggattttctg aggccagcgt gttcaccacc accgtggtac
     1381 tgttacgttt tgctttcagc tggattgtgc agttctgtac cggttttcct gtgccgtctt
     1441 tcagtacacc tgaaatcttt actgccatat tcaccccaca aaaaagcccg cctgaaccgg
     1501 cgggctgtca taacactgtg ttacctggct aatcagaatt tataaccgac acccacgatg
     1561 aaaccgtcag tgcgccagtc gccactgccg gaaccttcat aagcgacatc aacggccacg
     1621 gattcggtcg ggttaaactg cacgccagcc ccccacgcca gagacgtgtt gctgtggcga
     1681 ccgtcatcac ttccgatcag cacatcgtgc gttttccccg tattgtcggt cacctgcaga
     1741 taatccccgg agaaagtcga cacacggctg taagccacac ccgccatcgc atacgcgctg
     1801 aaccattcat tcacgcgcac agacggcccc gccatcacgc tgaaccagcg gttacgcacg
     1861 gaatc
//
LOCUS       DAJOWB010000312         1849 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJOWB010000312 DAJOWB010000000
VERSION     DAJOWB010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.319"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..249
                     /gene="tnpB"
                     /locus_tag="OHO85_005006"
     CDS             <1..249
                     /gene="tnpB"
                     /locus_tag="OHO85_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCQ2674213.1"
                     /translation="LKDDPFSGHLFIFRGRRGDQIKVLWADSDGLCLFTKRLERGRFV
                     WPVTRDGKVHLTPAQLSMLLEGINWKHPKRTERAGIRI"
     gene            269..1840
                     /locus_tag="OHO85_005007"
     CDS             269..1840
                     /locus_tag="OHO85_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCQ2674214.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTYITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKVDLSSQ"
BASE COUNT          444 a          507 c          537 g          361 t
ORIGIN      
        1 ctgaaggatg acccgttctc cggacacctg ttcatcttcc gcggacgccg gggtgaccag
       61 ataaaagtgt tgtgggctga cagtgacgga ctgtgcctct tcaccaaacg cctggagcgg
      121 ggccgcttcg tctggccagt cacccgtgac ggcaaggtgc accttactcc ggctcagtta
      181 tccatgcttc ttgaaggtat caactggaag cacccgaaac gaacggaacg cgctggaatc
      241 cgcatataac ccgttgtaaa gtgaggatat ggacacctca cttgctcatg agaacgcccg
      301 cctgcgggca ctgttgcaga cgcaacagga caccatccgc cagatggctg aatacaaccg
      361 cctgctctca cagcgggtgg cggcttatgc ttccgaaatc aaccggctga aggcgctggt
      421 tgcgaaactg caacgtatgc agttcggtaa aagctcagaa aaacttcgtg caaaaaccga
      481 acggcagata caggaagcac aggagcgaat cagcgcactt caggaagaaa tggcggaaac
      541 gctgggtgag caatatgacc cggtactgcc atccgccctg cgccagtctt cagcccgtaa
      601 accgttaccg gcctcacttc cccgtgaaac ccgggttatc cggccggaag aggaatgctg
      661 tcctgcctgt ggtggtgaac tcagttctct gggatgtgat gtgtcagagc aactggagct
      721 tatcagcagc gcctttaagg ttatcgaaac acaacgtccg aaacaggcct gttgccggtg
      781 cgaccatatc gtgcaggcac cagtaccttc aaaacccatt gcacgcagtt atgccggagc
      841 ggggcttctg gcccatgttg tcaccgggaa atatgcagac catctgccgt tataccgcca
      901 gtcagaaata taccgtcgtc agggagtgga gctgagccgt gccacactgg ggcgctggac
      961 aggtgctgtt gctgaactgc tggagccgct gtatgacgtc ctgcgccagt atgtgctgat
     1021 gcccggtaaa gtccatgctg atgatatccc cgtcccggtc caggagccgg gcagcggtaa
     1081 aacccggaca gcccggctgt gggtctacgt ccgtgatgac cgtaacgccg gttcacagat
     1141 gcccccggcg gtctggttcg cgtacagtcc ggaccggaaa ggtatccatc cacaaaatca
     1201 cctggccggt tacagcggtg tgcttcaggc cgatgcttac ggtggttacc gggcgttata
     1261 cgaatccggc agaataacgg aagccgcgtg tatggctcat gcccggagaa aaatccacga
     1321 tgtgcatgca agagcgccca cctacatcac cacggaagcc ctgcagcgta tcggtgaact
     1381 gtatgccatc gaggcagagg tccggggctg ttcagcagaa cagcgtctgg cggcaagaaa
     1441 agccagagcc gcgccactga tgcagtcact gtatgactgg atacagcaac agatgaaaac
     1501 actgtcgcgt cactcagata cggcaaaagc gttcgcatac ctgctgaaac agtgggatgc
     1561 actgaacgtg tactgcagta atggctgggt ggaaatcgac aacaacatcg cagagaacgc
     1621 cttacgggga gtggccgtag gccggaaaaa ctggatgttc gcgggttccg acagcggtgg
     1681 tgaacatgcg gcggtgttgt actcgctgat cggcacatgc cgtctgaaca atgtggagcc
     1741 agaaaagtgg ctgcgttacg tcattgaaca tatccaggac tggccggcaa accgggtacg
     1801 cgatctgttg ccctggaaag ttgatctgag ctctcagtaa atatcaata
//
LOCUS       DAJOWB010000313         1804 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJOWB010000313 DAJOWB010000000
VERSION     DAJOWB010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.320"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            9..443
                     /locus_tag="OHO85_005008"
     CDS             9..443
                     /locus_tag="OHO85_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HCQ2674215.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPK"
     gene            537..803
                     /locus_tag="OHO85_005009"
     CDS             537..803
                     /locus_tag="OHO85_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674216.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAQDEIN
                     PDVLAMSGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            868..>1804
                     /locus_tag="OHO85_005010"
     CDS             868..>1804
                     /locus_tag="OHO85_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCQ2674217.1"
                     /translation="MSQKKTTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGRMEGRMEGAL
                     EKALAIACQLQKMGMTPEQIKQATGLSDDELKKITH"
BASE COUNT          424 a          488 c          518 g          374 t
ORIGIN      
        1 acgttttcat gacagtcagc agcacgattt ccgttttttg ccgcgacggg gtgttccgta
       61 ccgtttactg ccacctgcac ggggagccga cctggaacgg tcgcatcctg catacccact
      121 atgccaccgg tcagcaggcc gaagccctgg ttgaacacgg tgatatccgt tgcctcggtc
      181 cccgttgcga caaacccgcc gggcatacgc ttcagaaccc ggtggacggt gtgacggctt
      241 attacggacg tgacagtggt ttccggatgg acagtgaggc gcgtgagtac cgttctttca
      301 gggaggctat tgccactgaa agcactgaag aggtgcgctt ccattatgtg ttcatcgacg
      361 gctactggaa ggtgatgtac cgcacgccgg aaggctggaa gatgaaagcg ctcgcgctgg
      421 cactgcgtcg ctgtccgaaa tgaaaaaaag gcagggcgat aaaccctgcc tctctccgcc
      481 ggcgcttccc cgccaggaag tccggcatct caatcactat ctatggagat tatgccatga
      541 gaccatcaat tatcttcgca accgccgagt atgtaaagcg tctgcgtgaa gagtgcctgc
      601 gggagaataa acccctgcac cgccataccc gcttcagacg tcaggagctg gcacaggatg
      661 agattaaccc ggacgtcctg gcgatgagcg gccatatcgc cagacgctgc agtgagcaga
      721 agcgggtgcg tatccccgct atgaaagtca gcgaatgggg ccacctgctc cgcgcgcttg
      781 aaattgagcg ggtctgccac tgattaaccc ttgccgtctg cctgtgcagg cggcaggata
      841 cgttcagaca tctcaccagg aggaaccgtg agtcagaaga aaaccaccac gacgcccacg
      901 ccgcatgatg ccgcgttccg gtcgttcctg gcgaatcccg acgtcgccag agattttctg
      961 gaactgcatc ttccggcgga gtaccggcag ttgtgcgacc tgtccacgct gaagctggaa
     1021 cccgccacct ttgttgagcc ggacctgcat cagtacgcca gcgatatcct ctggagtgtg
     1081 aaaaccaccg ggggtgaaga tggctatgtt tatacgctca tcgagcacca gagcaccgaa
     1141 aatctgtaca tgcctttccg catgttacgt tacagtgtgg cggcgatgca gagacatctg
     1201 gagcagcaca aaacgttgcc actggtgatt cctgtactgt tctatcatgg tgagcgcagc
     1261 ccgtacccgt acagcatgaa ctggctggac tgttttgaga atccggcact tgcggctaaa
     1321 atatacacaa agccgtttcc gctggttgat atcactgtcg ttgatgacaa tgaaatcatg
     1381 aaccatcgcc ggatggccgc actgacgctg ctgatgaagc atatccgcca tcgtgacatg
     1441 atggagctgc tggacaaact cccgcaggtc atggtggaaa tttcagacga gcaggtgcgt
     1501 gttctgattc attacatcgt taacgcaggg gactctgtat caccggaatt tatgcgggcg
     1561 ctggctgagc gtctgccgca gcatgaggat aaactgatga ctatcgcaga acgtcttgag
     1621 caaaaaggtc gccaggaggg caggatggaa ggacgcatgg aaggacgcat ggaaggacgc
     1681 atggaaggag cgcttgaaaa agccctggct attgcgtgcc agcttcagaa aatggggatg
     1741 acgccggagc agattaagca ggctaccgga ctttccgatg acgaactgaa gaaaatcact
     1801 cact
//
LOCUS       DAJOWB010000314         1770 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJOWB010000314 DAJOWB010000000
VERSION     DAJOWB010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.321"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(403..669)
                     /locus_tag="OHO85_005011"
     CDS             complement(403..669)
                     /locus_tag="OHO85_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674218.1"
                     /translation="MITGNGINTVTVNGKVKHITELDDITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
     gene            complement(669..1163)
                     /locus_tag="OHO85_005012"
     CDS             complement(669..1163)
                     /locus_tag="OHO85_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674219.1"
                     /translation="MCDSEAEKILFILNKHERAYRRLIPVYIFLAIVLWILPILLIVY
                     LYFCIVDNEFLRIGFCIAWTAYYFLSLFKYFVKYFFPLDREDLLKLMELAKDSPDAKQ
                     ELLERLLSGKRMTGRDEMDIKRILKTKEALDNIRNFIDKQQLTNSDTPKKSTVPNEDK
                     KGIR"
     gene            complement(1219..>1770)
                     /locus_tag="OHO85_005013"
     CDS             complement(1219..>1770)
                     /locus_tag="OHO85_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HCQ2674220.1"
                     /translation="RRTFSLPSLSEKTDIIAPVFTEQTAESAPAGINSSAVETHIPEA
                     PARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEGGGFIPLKIGINNDISAF
                     LAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGLDGHPKGQVSDSEAQSAG
                     RRLATLEQKWLRMQAQQENISGQ"
BASE COUNT          457 a          378 c          365 g          570 t
ORIGIN      
        1 gtgcgttaaa gtatcactgg tataatctgg taaagaacat attgagaatg agaacttatc
       61 atgtcgtcac gcaactctgt acatgatttt ataatgctgc tcccggcgct gctgttggtt
      121 gggtggttag ttttggtgat aattctcatg tttagggatg ataaatgtga caaaacttac
      181 cgtgaaggtt cgtttcccga atttatttgt ctccattctg atcgctcgtc acttcgagaa
      241 accgattctg agagactcat tgtcaaatat acagaaataa aatcacaaaa ataggtaaaa
      301 ccaataatac tatagctaaa aaatagttac ttttctgtat caccctgtct ctctctgctt
      361 tttcatggct actgtcatat ttatgtctgt tgcgcggttt tgttactttt tcatgaccga
      421 acccgaaatt ttgcgcgtca ataagagacg ttgtgtaaac tcagtccttt tatctggcag
      481 gttaacccct atcaatctta ggatgaaacc acgccatcct ctgtttgcct ggttattaat
      541 ttcataaaga cgattgtttt cctctcgtag ttttgtccac tctaaacaaa gtgttatatc
      601 atccagttct gtgatgtgtt tcacttttcc attgacagtt acagtgttta ttccatttcc
      661 tgtgatcatc atcttatgcc ttttttatct tcatttggga ccgtgctttt cttcggagta
      721 tcactattcg ttaactgctg tttatcaata aaattcctga tgttatcaag tgcttccttt
      781 gtttttagaa tgcgcttgat atccatttca tcccgcccgg tcattctttt tccggacaaa
      841 agacgctcaa gaagctcctg tttcgcatca ggagaatctt tggcgagctc catcagttta
      901 agcaaatctt cacgatcgag aggaaagaag tattttacaa aatacttgaa aagcgaaaga
      961 aagtagtatg ctgtccatgc aatacagaaa ccgattctga gaaactcatt gtcaactata
     1021 cagaaataca ggtatacaat caggagaata ggtaaaatcc ataatactat agctaaaaaa
     1081 atatatacgg ggatcaggcg tctgtatgcc cgttcatgtt tattcaggat gaataaaatc
     1141 ttctccgctt ctgaatcaca cataacattc tcttcccatt ttttctgata cgtaatgtcc
     1201 acctgttatc aggagtgttt attgcccaga aatattctcc tgctgtgcct gcatcctgag
     1261 ccacttttgt tctaatgtcg ccagtctgcg accggcactc tgtgcttcag agtctgagac
     1321 ctgccctttg gggtgaccat ccaggccata acgtgggacg ccggcgacag ctgtccgctg
     1381 cagatatacg cggcgggatg tgatgcagga cactgcacag agccattcgt ccatggtcag
     1441 ttctgtctcc gggtgttctg ccagaaatgc cgaaatatca ttgtttatgc caattttcag
     1501 gggaataaat ccaccacctt cagcacgaag atgaggaaac agggccttta ttttttccac
     1561 gcactcgtga gtatactcac gggtccagtg ggagggacgt ggaaaacggt gtcttttctt
     1621 tttcttcttc ctggctggcg cttcggggat atgtgtttcg acggcagatg aatttattcc
     1681 ggcgggggct gattctgccg tctgttcagt aaatacaggt gcgataatat ctgttttttc
     1741 ggataaggac ggtagcgaaa aagtacgacg
//
LOCUS       DAJOWB010000315         1709 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJOWB010000315 DAJOWB010000000
VERSION     DAJOWB010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.322"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            103..786
                     /locus_tag="OHO85_005014"
     CDS             103..786
                     /locus_tag="OHO85_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HCQ2674221.1"
                     /translation="MSEQKLIAESRTFEQQMIERDKRATKAGFVVGGMGLLIAVLALV
                     AVVVMLPLKQTDVELYTLDNHTGRVEHVTRTSKTSLTATEAYQKAMAANYVKVRERYV
                     YPSLQDDYETVQVYNAPQVNDDYLALYAGKNAPDKVYKNGAHTVKVEILSNQITDATA
                     PDRVATIRYKKIIRRLADNSTRNEYWDARFTFHSNPDKEMSDAEREINYFGFTVTSWQ
                     TDREIRGGE"
     gene            783..1691
                     /gene="virB9"
                     /locus_tag="OHO85_005015"
     CDS             783..1691
                     /gene="virB9"
                     /locus_tag="OHO85_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="HCQ2674222.1"
                     /translation="MMKKLTFTALALMMCGAAWAAAIPQASRYDSRVQQVIYNPQNVT
                     VVNTKPGFMTTLVFDNDEAVISAKPGFDEAWEATPDANRVNVRPVALTQGAPGEDGNT
                     TQVVIPPNSRDWHTNMLVVTSKRLYNVELNVIDDKSAQQPAFQVSYRYPGEERDKASR
                     EATARQREWEQKQQQASVQKALNSAQTPRNWSYTKYPGKGSFNIVPDFAYDDGRFTFV
                     GFSPSKSIPSVTKELNGKEHVVNSSIQKKGDFTVLVIQEVTPRLVLRSGNAVVGLENS
                     GFGKVHAADGSTVSRQVERVEKPESN"
BASE COUNT          450 a          462 c          467 g          330 t
ORIGIN      
        1 tgtgcgcgct ttctggctgc gcccagaaca cgcctccctc tgcctttagc ggtaagggag
       61 aacccgtaaa cacccctcaa attatggcgg agttaaacag tcatgagtga acagaaatta
      121 atagctgaat cccgcacttt tgagcagcaa atgattgagc gggataaacg cgccacaaaa
      181 gccggttttg ttgttggcgg tatgggtctg ctgattgcgg tactggcgct cgtcgccgtg
      241 gtcgtgatgt tgccgctgaa acagacggac gttgagctgt ataccttgga taaccacaca
      301 ggacgggtgg aacacgttac ccggacatcg aaaaccagcc tgaccgcaac agaagcctat
      361 caaaaggcga tggccgcaaa ctacgtcaaa gtgcgtgagc ggtatgtcta tccctctttg
      421 caggacgact acgagacggt acaagtctac aacgctcccc aggtaaacga tgactatctg
      481 gcgctttacg ccggaaaaaa cgccccagac aaggtttata agaacggggc gcacaccgtg
      541 aaagtggaga tcttgtccaa ccagatcacc gatgcgacag cccctgatag ggtcgccacc
      601 atccgctaca agaagattat ccgccgtctg gctgataaca gcacccgcaa cgaatattgg
      661 gatgcccggt tcacgttcca ttctaaccct gacaaggaaa tgagcgatgc ggaacgcgag
      721 atcaactact tcggctttac cgtcaccagt tggcagaccg accgcgaaat ccggggaggt
      781 gaatgatgaa aaagttaacg tttaccgcac tggcactgat gatgtgcggc gcggcatggg
      841 ctgcggccat accgcaggca agccgctatg actcccgcgt gcagcaggtt atctataacc
      901 cgcagaacgt cactgtcgtt aacaccaaac ccggctttat gacaacgcta gtctttgata
      961 acgatgaggc ggtcataagc gccaaacctg gctttgatga ggcgtgggaa gccacgccgg
     1021 atgcaaaccg ggtcaacgtt cgcccggtcg cgctcacgca gggagcgccc ggagaagatg
     1081 gcaacacaac gcaggtcgtg atccccccga acagccggga ctggcatacc aatatgctgg
     1141 ttgtcaccag taagcgcctg tacaacgtcg agctgaacgt catcgatgat aaatcggcac
     1201 aacaacccgc tttccaggtg agctatcgct atccgggcga agaacgggac aaagcgagcc
     1261 gggaggcgac ggccagacag cgcgagtggg agcagaaaca gcaacaggcc agcgtccaga
     1321 aagcgctgaa ctcggcgcag acgccccgca actggagtta caccaaatat ccgggcaaag
     1381 gcagtttcaa cattgttccc gattttgctt acgacgatgg ccgctttacg ttcgtgggct
     1441 tctccccgtc taagtccatt ccttccgtca ccaaagagct gaacggcaaa gagcatgtgg
     1501 ttaacagcag tatccagaag aaaggcgatt tcaccgtact ggtgattcag gaggtgacgc
     1561 cacgcctagt actgcgttcc ggtaatgccg ttgtgggtct ggaaaatagc ggatttggca
     1621 aggtacacgc cgccgatggt tccaccgtat cccggcaggt ggaaagagtg gaaaaaccgg
     1681 aatccaatta acctataaaa atattttta
//
LOCUS       DAJOWB010000316         1699 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJOWB010000316 DAJOWB010000000
VERSION     DAJOWB010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.323"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            132..1649
                     /locus_tag="OHO85_005016"
     CDS             132..1649
                     /locus_tag="OHO85_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCQ2674223.1"
                     /translation="MAGDGLVDGSRFIFDEVQLPEDRLQRYPLLEEMAVYSTIATALN
                     IHITHALSFDKKTGQTFSIVPVHHGNDSDYDAAQALCDELMNDIGRTINKEVAGWAFI
                     MSVFGVAYVRPYAKEGIGITSFECSYYTLPGFIKEFEVSGNLAGFSGDYLKDASGKMV
                     FADPWAIIPMKIPYWRPKSNLMPVHTGHKAYSLLDNPEERTPIETQNYGTSLLEYAYE
                     PYMNLRSAIRSLKATRFNASKIDRIIGLAMNSLDPVKAADYSRTITQTLKRAADLMEK
                     RARGANNMPTVTNTLLPIMGDGKGQMTIDTQTIQADINGIEDILTYMRQLAAALGLDY
                     TLLGWADQMSGGLGEGGFLRTAIQAAMRASWIQQGVEEFIQRAIDIHLAFKYGKVYPE
                     GDRPYKIEFHSVNTALQQEHNDNRDSQANYATIVTQILDAVSNNSVLANSDAFKRYLF
                     SDVLEIDEKISEALVNELKAKSEDDDHLMDSIIKTPPQELAQILESVFKEGNDND"
BASE COUNT          435 a          451 c          449 g          364 t
ORIGIN      
        1 acaacggaga acgggacaac atcctcctgt ccgcgctggc tgtgcacggc ggaagtgggt
       61 atttgttttc tcgcgcaggg gcaccggtac aactgtccgg cttcttaggc ggcaaaccgg
      121 gcgatagtgg catggctggc gatgggctgg tggacggaag tcgctttatc tttgatgaag
      181 ttcaactgcc ggaagaccgc ttgcaacgct atccgctact cgaagagatg gcggtttaca
      241 gcacgatcgc caccgcgctg aacatccata ttacgcacgc gctctctttc gataagaaga
      301 ccgggcaaac tttctctatc gtgccggtac atcacggaaa cgatagtgac tatgacgccg
      361 cgcaggcgtt gtgtgacgag ctgatgaacg acatcgggcg aaccatcaac aaagaggtcg
      421 ccgggtgggc atttatcatg tctgtatttg gggtggctta tgtcaggcca tacgccaaag
      481 aaggcatagg gatcacgtct tttgagtgct cctattacac ccttccgggc ttcatcaaag
      541 agttcgaggt cagcggcaac ctggcgggat ttagcggcga ttatctgaag gacgcgtcag
      601 ggaaaatggt tttcgccgat ccgtgggcca ttatccctat gaaaatcccc tactggcggc
      661 ctaagtcaaa ccttatgcct gtgcacactg gccataaggc ttacagcctg ctggataatc
      721 cggaagagcg cacaccgatt gaaacccaga attacgggac cagcttgctc gaatacgcct
      781 acgagccgta catgaatctg cgttcggcga tccgctcact gaaggcaacg cgttttaacg
      841 cgtcgaaaat tgaccgaatc atcggcctgg cgatgaatag tctggatccg gtaaaagcag
      901 ccgattattc acgcaccatt actcagacgc ttaaacgagc agctgacctg atggaaaagc
      961 gcgcacgcgg cgcgaataac atgcctacgg tgaccaatac tctgctgcct attatgggcg
     1021 acggcaaggg acagatgact attgatactc agaccatcca ggctgacatc aacggcattg
     1081 aagacattct cacctatatg cgccagctgg cggcagcact tggcctcgat tacaccctcc
     1141 tggggtgggc agatcaaatg tccggcgggc ttggtgaagg tggattcctg cgcacggcaa
     1201 ttcaggccgc catgcgcgcc tcatggatcc agcagggcgt agaagagttc attcagcggg
     1261 ctatcgatat tcatcttgct ttcaagtacg gcaaggtata cccggaaggt gatcgcccgt
     1321 acaaaatcga attccactcc gttaataccg ctctgcaaca agagcacaac gacaaccgcg
     1381 actcgcaggc gaactacgcc accatcgtta cgcaaatcct cgatgccgtc agcaataaca
     1441 gcgtcctcgc caattccgat gcattcaaac gttacctgtt cagcgatgtg ctggagattg
     1501 acgaaaaaat ctctgaagca ctggtgaacg aactgaaagc gaaaagcgag gacgacgatc
     1561 acctgatgga ttccatcatc aaaacaccgc cacaggaact ggcgcaaatc cttgaatcgg
     1621 tctttaaaga gggaaacgat aatgactgat gttttgaaaa cggtcactga ccgcttttgt
     1681 ctctatagca atgctcgaa
//
LOCUS       DAJOWB010000317         1691 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJOWB010000317 DAJOWB010000000
VERSION     DAJOWB010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.324"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..585
                     /locus_tag="OHO85_005017"
     CDS             <1..585
                     /locus_tag="OHO85_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HCQ2674224.1"
                     /translation="RTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFINEICHA
                     TDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNELAR
                     YGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLAYA
                     SDVYRIYTGIKSSKSIRIPTTKKSLPEINSVQNN"
     gene            complement(881..>1691)
                     /locus_tag="OHO85_005018"
     CDS             complement(881..>1691)
                     /locus_tag="OHO85_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCQ2674225.1"
                     /translation="SRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERYTYIP
                     TINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIILLNSH
                     DSSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFDKVTD
                     NIKAMKEIHLNSDEQHLFCRAALMVRYEDENKTPVTPEQTIIPRRREDKLNDLRTTWQ
                     RVQENMIKSGLSGRSTSGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
BASE COUNT          498 a          395 c          353 g          445 t
ORIGIN      
        1 agaaccacca acctgttgag cctttttgtg gaatgggtta aattatttac ggataaagtc
       61 accagaggtg gaaaaatgaa aaaatggatg ttagccatct gcctgatgtt tataaatgag
      121 atctgccatg ccactgattg ctttgatctt gcaggccggg attacaaaat agatcctgat
      181 ttactgagag caatatcatg gaaagaatcc cgttaccggg ttaatgccat cggtattaat
      241 ccggtaacgg gatatggcag cggactgatg caggtagatt cccagcattt taacgaactg
      301 gcccgctatg gaattaagcc ggaacatctg acaacagatc cctgcatgaa catttatacc
      361 ggtgcttatt atctggcaat agcctttaaa aaatggggcg tctcctggga ggccgttggt
      421 gcatataatg ccggattcag gaagaccgaa cgccagaacc agagacgtct tgcctacgca
      481 tcagatgttt accggattta taccgggata aaaagcagta aaagtatccg gattccgacc
      541 acgaagaaat cacttccgga aatcaacagc gtgcagaaca attaatctta cagacattgt
      601 cacataccgg ttatgccgca aaagcggcat ggaaggccac acacaaaaat atcatatgag
      661 tgacaatact gactggccga agccccgcga agcgggaacc catcttatat atacccggta
      721 actgcgcagc ctcctgccag accatttttc gcgggcacaa tatttacaga caaccggaaa
      781 gccagttgat gaaattgagg tatatcagac gacacagcga tgccgaagaa tgataatata
      841 agtaaagccc cgaaaaattt tcggggcttt acttatatta tcacgacttc cactttctga
      901 actgttcggc aatcacccat aacgccttgt tgattcttat atcaccatca atacctgtaa
      961 tggctcttgt cctggtattt ttaccggagg tacttcgccc cgataatcca ctttttatca
     1021 tattctcctg aacccgctgc caggttgtcc ggagatcgtt cagtttatcc tcccgacgac
     1081 ggggaataat tgtttgttca ggcgtcactg gcgttttatt ttcatcttca tacctgacca
     1141 tcagtgcagc tctgcaaaat aaatgttgct cgtcactgtt aagatgaatt tctttcatcg
     1201 ccttaatatt atcagtgacc ttatcaaaga caccgagtac ttcatatgct ccctcgataa
     1261 cctgcccgac aatatcacct ttatgtggaa cgcggatttc gccaaaatta tttccgcaca
     1321 ccaggccatt tgtgcagaca aaacgaaaaa ttccggggat catctgataa ctggatgaac
     1381 tatcatgtga attaagcagg ataatttcag gaacttcctg cccgttaata tgcccttccc
     1441 tgcgaagacg taacatatgt ttactgtatt cgcggcgtcc caaatcacga acccgactct
     1501 gacaggcaaa gaatggctgg aaaccttcat cacgtaactt attgatgata ttgattgttg
     1561 gaatatacgt ataacgttca ctccgggact catgtttatc accggaaaat accgaaggca
     1621 cgaactgcat taattcatca tccgttaaag gacgttcacg gcggatggaa ttataccgac
     1681 caaaacggga a
//
LOCUS       DAJOWB010000318         1687 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJOWB010000318 DAJOWB010000000
VERSION     DAJOWB010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.325"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..528
                     /locus_tag="OHO85_005019"
     CDS             <1..528
                     /locus_tag="OHO85_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCQ2674226.1"
                     /translation="AVKGINREQLQTMITESGIALDISCDNTPRQQVIGGTQASLNEF
                     ATLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYLAGLDIASPEHQVLMNVTAKSEV
                     APSIIKENLSLHLTHTVKWTESLDTFLNMPTPVAFLEISNKPYLGNMLNDFAGVDQQR
                     VMHCRKAFSDAKVFK"
     gene            525..1511
                     /locus_tag="OHO85_005020"
     CDS             525..1511
                     /locus_tag="OHO85_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674227.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRCLWHNDFMMACDMYFRWGHFNK"
BASE COUNT          413 a          328 c          405 g          541 t
ORIGIN      
        1 gcggttaaag gcatcaaccg cgaacaactg cagacgatga ttacggaatc aggcattgcg
       61 ctggatatta gctgtgataa cacgccgcgc cagcaggtta ttgggggaac gcaggcctcg
      121 ttgaatgaat ttgccacgct gctgatggcc gctggatacg aaccggttaa actcggtgtg
      181 agcggtgcct ggcacacgcg tttgatggaa gacggcgttc aggcaatgcg cgattatctt
      241 gccggactgg atatcgcttc gccagagcat caggttctga tgaacgtaac agcaaaaagc
      301 gaggtcgctc cgtcaattat caaagaaaat ctctcattac acttaaccca tacggtgaaa
      361 tggactgaat ctctcgatac atttctgaat atgccaaccc cggtagcttt ccttgaaata
      421 agtaataagc cctatttggg gaatatgtta aatgattttg ctggcgtgga tcagcaacga
      481 gttatgcatt gtcgtaaagc atttagtgat gcaaaggtat tcaaatgatt cctgattatt
      541 taacttttat tcgctttcag gataaacgaa atctgatata catttatgct attggactta
      601 ttctgatagg cttttattgg aaaaatgcag gatttacttt tccatcagag gatattggtg
      661 tcgttagtgg gattctggcc ctggtgctgt ataattttat ttttgatctc aaggcgtact
      721 gggcttataa atgcgtcacg aagaatatcg atttttcgtg gtttaagaaa aagcagaacc
      781 acaaaataga attatttctt acacaacctc tggtggcagg atttctgtcg ttaatcatgt
      841 tgagtgcaat gagttggggg ctataccagc ttctaccctc gttatatgcg ctgttcctga
      901 tttcgttact tgggccgttg gtcatctttc tgctgtttcg gatgatccgc accagttatg
      961 tcaagcaggt cgctatttca gtagcgaaaa aagtaaaata taaaagtctg actcgctatg
     1021 tgctgctttc ggtgtgcatc tcaacggttg ttaacctgct tactatcagc ccgttacgta
     1081 acagtgattc ttttgtgaca gaggggcagt ggttaacgtt taaatcgata attgcattgc
     1141 tcattctttg tggcgtagtg ttggcgatta atctgttttt tctgcgcttc tccaagcggt
     1201 acgcttttct gggcaggctt tttttgcagg aaatcgatct gtttttctcc agtgaaaatg
     1261 cgttgtcgac cttttttgcc aagccgcttt ggcttcggtt attcatattg ctggttattg
     1321 aaatgatgtg gattacgctg gtgtcggtat tggcaacgct tgtggaatgg cggatttggt
     1381 ttgaagccta ttttttactc tgctatgtac cgtgcttaat ttactatttt ttccattgtc
     1441 gatgcctctg gcataacgat tttatgatgg catgtgacat gtatttccgt tgggggcatt
     1501 ttaataagtg aggaagttac aggaagtgac cagataacgc atatatgttc tctactatct
     1561 tgcgcatttt gattgttgac tgagtaacca gacagttgat gtgcacaatt ttccctcgcc
     1621 ctaacagacg tgggcggggg cacccggtgt gtgcaatata cgaaaaaaaa gcccgtactt
     1681 tcgtacg
//
LOCUS       DAJOWB010000319         1676 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJOWB010000319 DAJOWB010000000
VERSION     DAJOWB010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.326"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(39..1064)
                     /locus_tag="OHO85_005021"
     CDS             complement(39..1064)
                     /locus_tag="OHO85_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HCQ2674228.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDEDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            complement(1090..>1676)
                     /gene="rfaY"
                     /locus_tag="OHO85_005022"
     CDS             complement(1090..>1676)
                     /gene="rfaY"
                     /locus_tag="OHO85_005022"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCQ2674229.1"
                     /translation="KVLRNIDDTKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGD
                     YYQNLIVETDRVRSAGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPE
                     NVKAEIKASMEKLHALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAME
                     RHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA"
BASE COUNT          526 a          339 c          276 g          535 t
ORIGIN      
        1 aattattatt gtaactcaga catccatgat ttttattatt atccaaatag ttttctttta
       61 tacttattta atttaaattt aatgaagtaa ataaaagcgt taaacgtatc accatttttc
      121 tttgcatgtt gatatttcac ttgatactgc ttttcattcg tggctggaat aaacgccaca
      181 tcattccagc aagaagcctg ataagcctca ttaaaatact tctggcaggg ataattcgtc
      241 cagtcgttcc agggcttcgt tacgccaaca taatgaatca gcttagtatc atcggtaatg
      301 gtctttttat aatcttcatc gcttttattt tttaactcat agtcaatgcc ataaaagcaa
      361 tcataatcgc cgctaataaa aatacaatga ccaaagaaca gaatatttaa aatatcctga
      421 tcaaggcatt tatacttcgt gtgctgcggg tcacttgcca gcatagcaat ggctttattc
      481 ataaaatcat ttttcgccca tgcttcaagc tgcagatata ctacaccggc gttgaaataa
      541 ttccctggaa gtccctctat agccagacgc ttcgctggtt tttcctgcat aaaaggcaca
      601 tctttaataa cagctgcgaa cttatcctca tcaaaattaa tatcaagcaa tccagctaat
      661 gagcctttac agatgacatc agcatcgata tataacagat tttcaatagt ttcactcaaa
      721 tactggaacg caactaaacg gaagaatgtt gcataagacc actgtttcag gctggggaag
      781 atatcaataa aggcattgga gactttataa attattactt caacgttagc atttttgctg
      841 gtaaaagcat taacacgttg tagataatct tcatcaatgt agtcggtaaa gagatgaaac
      901 ttaaccgcaa ggtccgggtt atgcataaca actgattgca tagatactgc cgcaccaaat
      961 aaaaagttac ggtcaatacc gtaagcgata tttaacacac gatcggtgtt agcaatttcc
     1021 ccagagacag cgacttcagt aatactctca gctaataaat ccatgttggt tccgttttga
     1081 ctgtgtggtt tacgctttgc cttttaattt tttaataaat ttgcgtagtt tggttctgta
     1141 gattactgaa tagtagccat aatcttttat ttcgttggca atacccaggt gacgttccat
     1201 ggccaggcga tcacgagctt tacgctctgc cgtacagctt ttaccggata ggtcaattat
     1261 cctgacacca tctttactaa cgataaagtt accccggtgt gggtcgcccg atagcatatt
     1321 taaggcatgc aatttttcca ttgaagcctt aatttctgct ttaacatttt ctggaataat
     1381 cggcatatca ttgagctcta cgccctcgac atactcgata agcatgataa aaacactggc
     1441 atagttgaaa attttacgct cagccagaaa ataaaaatca ttaggaaaag taagacctgc
     1501 acttctgaca cgatcggttt caacgatcaa attctgatag tagtcacctt ttacaaaaga
     1561 ttttaaaaaa cgttcattcc ttttcgtttt cggagcaaag actttaaaaa catagcgacc
     1621 atatttcgta tcaatcaacg atactttggt atcatcaata ttacgtaata ctttta
//
LOCUS       DAJOWB010000320         1658 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJOWB010000320 DAJOWB010000000
VERSION     DAJOWB010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.327"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..669
                     /locus_tag="OHO85_005023"
     CDS             <1..669
                     /locus_tag="OHO85_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCQ2674230.1"
                     /translation="AMLQNVSREELLEEFIVPSVVPGVDVMPASIDDAFIASDWRELC
                     NEHLPGQNIHAVLKENVIDKLKSDYDFILVDSGPHLDAFLKNALASANILFTPLPPAT
                     VDFHSSLKYVARLPELVKLISDEGCECQLATNIGFMSKLSNKADHKYCHSLAKEVFGG
                     DMLDVVLPRLDGFERCGESFDTVISANPATYVGSADALKNARIAAEDFAKAVFDRIEF
                     IRSN"
     gene            686..>1658
                     /locus_tag="OHO85_005024"
     CDS             686..>1658
                     /locus_tag="OHO85_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCQ2674231.1"
                     /translation="MSKKNRPTIGRTLNPSILSGFDSSSASGDRVEQVFKLSTGRQAT
                     FIEEVIPPNQVESDTFVDQHNNGRDQASLTPKSLKSIRSTIKHQQFYPAIGVRRATGK
                     IEILDGSRRRASAILENVGLRVLVTDQEISVQEAQNLAKDVQTALQHSIREIGLRLMR
                     MKNDGMSQKDIAAKEGLSQAKVTRALQAASAPEELVALFPVQSELTFSDYKTLCAVGD
                     EMGNKNLEFDQLIQNISPEINDILSIEEMAEDEVKNKILRLITKEASLLTDKGSKDKS
                     VVTELWKFEDKDRFARKRVKGRAFSYEFNRLSKELQEELDRMIGHILR"
BASE COUNT          467 a          344 c          407 g          440 t
ORIGIN      
        1 gctatgttgc agaatgtaag ccgtgaagag ctgttagagg agtttattgt tccttctgtt
       61 gtacctgggg ttgacgttat gcctgcgtcg attgacgatg cctttattgc atccgattgg
      121 agagagctgt gcaatgagca tctaccgggt cagaacatcc atgctgtcct gaaagaaaat
      181 gtgattgata agctgaagag cgattatgac tttatcctcg ttgatagtgg tcctcacctt
      241 gacgccttcc tgaaaaatgc tttggcctcg gccaatatac tgtttacacc tctgccgcca
      301 gcaactgtcg atttccactc atcgcttaaa tacgttgccc gccttcctga gttggtaaaa
      361 ctcatttcgg atgaaggctg cgagtgccag cttgcgacta acattggttt tatgtccaag
      421 ttgagtaaca aggcagatca taagtattgc catagcctgg ctaaagaagt gttcggtggg
      481 gatatgctcg atgtcgtcct ccctcgcctt gacggttttg aacgttgcgg cgagtctttt
      541 gacactgtta tttcagctaa cccggcaacg tatgttggta gtgctgatgc attgaagaac
      601 gcgcgaattg ccgcggaaga ttttgctaaa gcagtttttg accgtattga atttatcaga
      661 tctaactgag gagtaagaac cccccatgtc aaagaaaaac agaccaacaa ttgggcgaac
      721 ccttaatcct tcaatattaa gcggatttga tagttcttca gcctctggcg atcgagtcga
      781 gcaggtattc aagttatcaa ctggtcgcca ggccacattt attgaagagg taatacctcc
      841 gaaccaggta gaaagcgata cctttgttga tcagcataac aacgggcgtg accaggcatc
      901 tcttacgcca aaatcattaa aaagtatccg aagcactatt aagcatcagc aattttaccc
      961 tgcaataggt gttagacggg ctacagggaa aattgaaatt ttggatggtt cccggcgtcg
     1021 agcttctgcc atcttagaga acgtagggtt gcgggtttta gtcacggacc aggagatcag
     1081 cgttcaggaa gcgcaaaatt tagcgaaaga cgttcagaca gcattgcagc acagcattcg
     1141 agaaataggt ctgcgtttga tgcgaatgaa aaatgatggg atgagtcaga aggatattgc
     1201 agccaaagaa gggctgtctc aggcgaaggt cacgcgtgct ctccaggcag cgagtgctcc
     1261 ggaagaatta gtcgcccttt tccctgttca gtcggaatta accttttcgg actacaaaac
     1321 gctttgtgct gttggcgacg aaatggggaa caagaattta gagtttgatc agcttattca
     1381 aaacatatcc ccggaaataa acgacatctt atccattgaa gaaatggccg aagatgaagt
     1441 taaaaataaa atcctgcgct tgataacaaa ggaagcctca ctactcacgg ataaaggttc
     1501 taaagataag tccgtagtta ctgaattatg gaaatttgag gacaaggatc gctttgcaag
     1561 gaagcgcgtg aaaggccgtg cattttctta tgagtttaat cgactttcaa aagagctaca
     1621 ggaagaactc gacaggatga ttgggcatat ccttagaa
//
LOCUS       DAJOWB010000321         1640 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJOWB010000321 DAJOWB010000000
VERSION     DAJOWB010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.328"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(84..242)
                     /locus_tag="OHO85_005025"
     CDS             complement(84..242)
                     /locus_tag="OHO85_005025"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2674232.1"
                     /translation="MAQNAFIESFNRTYRTKILGFCLFRTLDEKRELAANWLSEYNSE
                     RHINYLTI"
     gene            complement(474..964)
                     /locus_tag="OHO85_005026"
                     /pseudo
     CDS             complement(474..964)
                     /locus_tag="OHO85_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          442 a          389 c          348 g          461 t
ORIGIN      
        1 tgcgttgtaa acagactcgt tttagttcca tgcacctttt gagatcgtga ccaaataatg
       61 gcgttagtga tgttcttcca gtgtcatatg gttaagtagt tgatatggcg ttcactgtta
      121 tattctgaca accagtttgc cgccagttcc cgcttttcat ccagcgttct gaacagacaa
      181 aaaccgagta tttttgttcg gtatgtccgg ttaaaactct cgataaaagc gttctgcgcc
      241 aacttacctg attgggtaat ctccagtttt actacatgtt tttctgcgca ttacgtccgt
      301 gttggataaa aaaaatcctg gccataatcc atgcgaatca tagctggata gacatggttt
      361 tccgcaatcc tacagagtac cgctaccact tgtgaagctg gcagattcag attgttttca
      421 atcgacaacg cctcatagtt aaagtcatca acgacattga acgtgtgaag tggtcaacaa
      481 aaactggcca ccgagttaga gtttttccag tatcgatttt ccgattcgtt tggggataac
      541 ccaccgttat attcgtgcgg tcttagtgcg ctgtaatatc caacgatata gaccgttatg
      601 gcgtgagcgg cctcgcagaa gcttcgtaac ccaccaccgg catccattcg ttcttcagac
      661 tcctgaagaa gcgttccatt gggctgttat cccagcagtt tccgctccgg ctcatactct
      721 gcctgatctg gtatcgccac aataactgcc ggaactgcct gctcgtatcg ctgtggaaca
      781 tcaccccgcc gggcttacca cgggtttccc atgccatttc cagcgctttc atggtgagcc
      841 tgctgtccgg cgagaacgac atggcccagc ccactggttt tcttgcgaac aggtcgagaa
      901 caacggcgag gtacgcccgg cgcttacccg tccagatata ggtcacatca ccgcaccaca
      961 cctgtatagc ggcgtaatcc ccaaaaaaga tgctggttgt cagtaaacaa gccatgaatg
     1021 acagtcgttc ctgaaatcat aagacctgga ggagtgtatg ctccgtcgca gtaattacca
     1081 cgatacgctt gcagaaattt tttccagtta ctgaagcgtg aacggataaa gaaaaagctc
     1141 ttcggaacgc ggggaaacgc ctccaacgat atttttggac atcgaaatat tttataacaa
     1201 taagcgtcgg catggttcct gcgaacagat gtcaacaata gaatataaaa gcaattatta
     1261 tcaacaactt ggaagaagta agactacccg tggcgattca atattagatc attgatggag
     1321 ttataaatgg ctcacttttt atcggttgtt gacgatacgt ttatcaagta tgcatgaaaa
     1381 tttcttcata ccagctatat gagacgttat gactaataat cttacttccc aaatgactat
     1441 gttgttgcag tttggtttat tgactcttgt ctatagttca ccgaatgcca agatgagggc
     1501 gcgaggaact ttactgtcta cacctctctt cctggttaca aagacaagcg taaaactcac
     1561 catccattag aactaaaggc actttcctga gcagagtttt catctgcaag ataaaagcta
     1621 ttttacagcc gtaaagtcca
//
LOCUS       DAJOWB010000322         1632 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJOWB010000322 DAJOWB010000000
VERSION     DAJOWB010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.329"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..1632
                     /locus_tag="OHO85_005027"
                     /pseudo
     CDS             1..1632
                     /locus_tag="OHO85_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
BASE COUNT          575 a          319 c          301 g          437 t
ORIGIN      
        1 ccttccgaac agcaagaaac atttaaaata ctaatcaatc cagaacatct acggcaaaca
       61 cttgagcgta aatctcttac tgctgttcag aaatcacaat gtgaaatcat aacccccaaa
      121 aaacctggcg aagcgattct tcatgctttt aatgccacct accagcaaat cagggaaaat
      181 atgtctgagt ttgcacgttg ccattatggg tatatacaaa tccctccagt gacaactttc
      241 cgcgccgacg gaccagaaac tcccgaagaa gaaaagggtt actggtttca tgcttatcaa
      301 cccgaagatc tttgtaccat ccataatcca atgggagatt tgcaggattt tattgcattg
      361 gttaaagatg ctaaaaaatt tggtatcgat atcattcctg attatacctt taactttatg
      421 ggaattgggg gtagtggtaa aaatgacctg gattatccct ctgctgatat acgagcgaag
      481 atcagtaaag atatagaagg tggtatccct ggctattggc aaggtcaggt tttgattcca
      541 ttcattaaag atccagtaac aaaagaacgt aaacaaatcc atccagaaga tatacatctc
      601 actgcaaaag acttcgaagc aagtaaagat aacatctcta aggatgaatg ggaaaacctc
      661 catgcattaa aagaaaagcg tttaaatgga atgcctaaaa caacacccaa aagtgaccag
      721 gttattatgt tgcaaaatca atacgttcgt gaaatgcgaa aatatggcgt acgaggttta
      781 cgttatgatg cggcaaaaca ctcaaaacat gaacaaatag aaagatcaat aaccccaccg
      841 cttaaaaatt ataatgagcg gttacacaat actaacttat ttaacccaaa atatcataaa
      901 aaagccgtta tgaattacat ggaatatctg gtaacttgtc agttggatga acaacaaatg
      961 tcatcactgc tttatgaaag agatgattta agcgccattg atttttcatt gctcatgaaa
     1021 acgataaaag ccttttcatt tggtggagat cttcaaaccc ttgcatcaaa accgggttcc
     1081 acaatctcaa gtatcccatc agaaagacgg atattgatta acattaacca cgattttcct
     1141 aacaatggta atcttttcaa tgactttcta tttaaccatc aacaagatga acaattagca
     1201 atggcatata tagccgctct gccgttcagc aggcctttag tttactggga tggccaagta
     1261 ttaaaatcaa cgactgaaat taaaaattat gatgggtcca cgcgtgtcgg cggtgaggcg
     1321 tagcttaata aaggttgctc tacctatcag cagctctaca atgaattcca cgcattatat
     1381 atagataaag caggaatatg gagcgcattt gagggtgtat ctgcaactaa aaacgttctg
     1441 gcctttagtc gtggggattc tgtgaacatt aatcactctc ctcatgatgg actagttata
     1501 ataaataaag gaaacgaaga agttgaaggt acctggccta acaaattgca acctggaata
     1561 tacaaaaaca tggggagtaa tagcgttaac attattatta ataatacccg aaaaattatc
     1621 ccccctggta aa
//
LOCUS       DAJOWB010000323         1625 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJOWB010000323 DAJOWB010000000
VERSION     DAJOWB010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.330"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(47..>1625)
                     /gene="espL1"
                     /locus_tag="OHO85_005028"
     CDS             complement(47..>1625)
                     /gene="espL1"
                     /locus_tag="OHO85_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HCQ2674233.1"
                     /translation="TTPIKANHLRSILDYIEQEQLTAENRNHCMKLSKKIHREKTIQP
                     TVNLNGSAFFLQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLV
                     RDAEFRALLRHPPAGSRVIASKDFGFALDIFFGRMMANNVSHMSAILYIDNHTLSVRL
                     RIKQSAYGQLNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVR
                     NCAIAILPLLPEGVPGAIFTGIATRMPFAPIHPSAMLLIMATGQTQQLIILFRQLHIL
                     PEKEIIEIITAQNSVGTPALFLAMMNGHTDNVKIFIQEIQSLVDIHIIHEDNLVKLLQ
                     TKSANETPGLYISMLYGFDEIIDIFLNALTTPIAQELLNKKLVMSILAMKTHDGEPGL
                     YAAMENNHPLCVTRFLSKINGIAFKYKLSKANIMDLLKGATAQGTPALYIAMSKGNED
                     VVLSYISTLGAFAKKHSFSQHQLFTLLAAKNHGNMSAVHIAIHHNHYKTVETYYAAIN
                     AISQSLSFSADELKTYL"
BASE COUNT          475 a          306 c          339 g          505 t
ORIGIN      
        1 aaaaaataaa attatgtatc gatatacaaa tatcataaat agctggttat aaatacgtct
       61 ttagttcatc agcactaaaa ctcaggcttt ggctgattgc attaatagca gcataatatg
      121 tttctacagt tttataatga ttatgatgaa tggctatatg aacagctgac atgttgccat
      181 gatttttagc agccaacagt gtaaataact gatgttgact aaaagaatgt ttttttgcaa
      241 aagcacccag cgtcgatata taagataaca cgacgtcttc attacccttg ctcatggcga
      301 tgtataatgc aggggttccc tgtgctgtag ctccttttaa taaatccatg atgttagctt
      361 tgctcaactt gtatttaaag gcgatgccat taattttaga gaggaaccgt gtgacacaca
      421 aagggtgatt attttccatt gcggcgtata atcctggctc accatcatgt gttttcatgg
      481 ctaagatact catcaccagt tttttgttta aaagctcttg tgctattgga gtggttaatg
      541 cattcagaaa gatatcgatt atctcatcga atccatacaa catggagata taaagtccag
      601 gtgtttcgtt agcactttta gtttgcagta atttaaccag attatcttca tgaatgatgt
      661 gaatatctac cagtgactga atttcttgta taaatatttt cacgttgtca gtatgtccgt
      721 tcatcatagc caaaaataaa gcaggtgtac caacgctatt ctgcgcagtt attatttcaa
      781 tgatttcttt ttcagggagt atgtgcaact gtctgaataa tataataagc tgttgagtct
      841 ggcctgtggc cattattaac aacattgccg atggatgtat aggggcaaat ggcattcgtg
      901 tcgcgatacc cgtgaaaata gcccctggaa ctccctcagg taataggggc agaatagcaa
      961 ttgcacagtt gcgaacatac ctatcagccc aggtctgagc atcgggacct gaactgatga
     1021 acttatcaag cgagagaaag ccccgtgctg tcctgtgggt gcctctgacg gcaacgttgg
     1081 tatcgttcgg gtcgtaaacg gacacaacat agtttaattg cccatacgcc gactgcttta
     1141 ttcgtagcct tactgacaaa gtatgattgt ctatatataa aatcgcggac atatgactga
     1201 cattgtttgc catcattcga ccgaagaaaa tatctaaagc gaagccaaaa tccttactcg
     1261 cgataacgcg actccctgca ggaggatggc gaagtaatgc tctgaactcc gcgtcccgga
     1321 ctaaggggac atgctgctgt atggattcaa cggaggagta atgtttaatc agattgactt
     1381 tgccttttcc atttctcagt gaatcaaggg cgtattgcaa tgacagatgg cgacaaaaaa
     1441 tagcgtcaga aggagattgc aaaaaaaatg cactaccatt gagatttact gttggttgta
     1501 tagttttttc tctatggatt tttttagaca gtttcataca atggttacga ttttcggcag
     1561 ttaactgttc ctgttcaata taatccagta tgcttcgcaa atggttagct ttaataggcg
     1621 tggtt
//
LOCUS       DAJOWB010000324         1622 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJOWB010000324 DAJOWB010000000
VERSION     DAJOWB010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.331"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            173..>1622
                     /gene="istA"
                     /locus_tag="OHO85_005029"
     CDS             173..>1622
                     /gene="istA"
                     /locus_tag="OHO85_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HCQ2674234.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEK"
BASE COUNT          449 a          384 c          381 g          408 t
ORIGIN      
        1 atcactcaat aacttccatc gagatcgggt aataacattt gaacagatcg ctgaataaca
       61 tcgatggaga tcacttttga ctcattttgt tattcagtga tctccatcaa tgttattgga
      121 acttcacagg tgtgttgatc tgtatctttt gccattccgg taaaggatac ctatgccaac
      181 agttccaatt tctatgagaa aacttaaaga aattcttagg cttaaatacg gtgttggact
      241 cagccatcga caaattggtc gtagtcttgc aatctcccct tccgttgtat ccagatatgc
      301 taatcgggcg gctcaacttg gcataaagca gtggccctta cctacaggat gggatgatac
      361 aaaactaaaa catgcgttcc ttcagaccca ggttaagatg aagaagcact ctctgcctga
      421 ctgggctaca gtacaccggg aactgcgtaa taaatgcgtg acgctgcagc tactctggga
      481 agaatactgt gagcgtaatc caggcggttt ttacagctat aaccattact gccggatgta
      541 ccgtgaatgg ctcaaaacca cttcaccatc aatgcgtcag gtacataaag ctggcgaaaa
      601 acttttcgtt gattactgtg gacctaccgt tggcgttacc gaccctgaga ccggagaaat
      661 aagaactgct caggtcatcg tagctgttct cggggcatca agttacacat gggcagaggc
      721 cacctggtct cagcagcttg aagactgggt gatgagtcat gttcgctgct tccagtggtt
      781 gggtggcgtt cctgaacttg ttgttccgga caatctgaaa agcgccacat ccagggcatg
      841 taagtatgat cctgacgtta accctaccta ccagcagatg cttgagcatt ataatgtcgc
      901 agttttgcct gcgcggccac gtaaaccgaa agataaagcc aaagctgaag ttggcgttca
      961 ggttgttgaa cgctggatca tggcccgaat caggcatgag atcttctaca gccttgcatc
     1021 gcttaatcag cgcattcggg agttgctgga aagactgaat aacaaaataa tgcagaagtt
     1081 gggttattca cgtgcagaac tcttcatcca gcttgataaa cccgcactga agcctcttcc
     1141 tgaagccagt tacagttaca ccctggtgaa gaaagtcaga gttcatgccg attaccacgt
     1201 ggaaatcgac aaacattact actcggttcc atgttcgctg ttaggccagc aactggaagc
     1261 atggatctcc ggagaactgg taagactctt caatcagggg caggaggttg ctgtgcaccc
     1321 gcgcaagcgt acttatggct acagtacccg caacgagcac atgcctgaag ctcatcgaca
     1381 gcatgccacc tggacgccag agcgtcttct ggaatgggcg gggcacatag gcagtgaaac
     1441 tcatagttat gtgcttcata tactgaactc tcgtccacat ccggaacaaa gctatcgctt
     1501 ctgccttgga ctcctgaacc ttcataaaaa atacagtaaa gccagactta atgcagcatg
     1561 tgcaagagct ctgaaaacaa aggtatggcg tctgtcaggt attaaatcga tcctggaaaa
     1621 ag
//
LOCUS       DAJOWB010000325         1586 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJOWB010000325 DAJOWB010000000
VERSION     DAJOWB010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.332"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..562
                     /locus_tag="OHO85_005030"
     CDS             <1..562
                     /locus_tag="OHO85_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HCQ2674235.1"
                     /translation="VWVKQALEKNQPGKPVRVINPDDESIRIFSWLADNFPDLQYFKL
                     QLLDASNLRLTVSKQRNAITQQLIDNLIKGLLQTMPYASNISIAVLDDNVLESQAIET
                     LSAIGLSYEKYKTANNVYFNIIGTLSDSELNKINNYVDEYYKQWGKQYVRFNVNLKNQ
                     DTNNSSFSYGDNRFEKSQGSNWTFQE"
     gene            576..816
                     /locus_tag="OHO85_005031"
                     /pseudo
     CDS             576..816
                     /locus_tag="OHO85_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            908..1123
                     /gene="sctI"
                     /locus_tag="OHO85_005032"
     CDS             908..1123
                     /gene="sctI"
                     /locus_tag="OHO85_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HCQ2674236.1"
                     /translation="MIDLNDRVLNLDNPDDKMISAFANYAVQTENWQQNALQALRSDK
                     EGLTPEKLLVLQDHVLNYNVEVSLVEH"
     gene            1164..>1586
                     /gene="sctJ"
                     /locus_tag="OHO85_005033"
     CDS             1164..>1586
                     /gene="sctJ"
                     /locus_tag="OHO85_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HCQ2674237.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTA"
BASE COUNT          542 a          262 c          323 g          459 t
ORIGIN      
        1 agtctgggtc aaacaagcac tggaaaaaaa tcaaccagga aagccagtac gggtgataaa
       61 tcctgatgat gaatcgatac gaattttttc atggcttgct gataatttcc ctgatttaca
      121 atattttaaa cttcaactat tagatgccag taatctaaga ttgacagtga gtaagcaacg
      181 gaacgctatc acacagcaac taatcgataa tcttattaaa ggattactgc aaactatgcc
      241 atatgccagc aatataagta ttgcggtatt agatgataat gtattggaaa gtcaggctat
      301 tgaaacgttg tcagcgatag gtctttctta tgaaaaatat aaaacagcta acaatgtgta
      361 tttcaatatc attggtacgt taagtgacag tgaattaaat aaaattaata actatgttga
      421 cgaatattat aaacaatggg gtaaacaata tgtaagattt aatgtgaatt tgaaaaatca
      481 ggacacaaat aatagttcat ttagctacgg agataaccga ttcgagaagt ctcaaggtag
      541 caactggaca tttcaggaat aattgaggag gacttatggc agattggaat ggttatatta
      601 tggatatcag caatcaattt gatcagggcg ttgatgatct gaaccagcaa gttgaaaaag
      661 cgttggagga tttagccaac caatccctcc gacacgaaat ttcttgccga atatcaaagt
      721 gcattagctg agtatacatt atatcgaaat gcgcaatcta acgttgtaaa agcgtataaa
      781 gatcttgatt cagcaatcat acaaaacttc cgctaaactg gataaggatt ttattatgtc
      841 agtaagcaat atgccaccta ttgatagagc tgagtaatca actgctcatg aaatacagca
      901 agcgaaagtg attgatttaa atgaccgggt tctcaatctt gacaacccgg atgataaaat
      961 gatctccgct tttgccaact atgctgtaca aactgaaaat tggcagcaaa atgcgttaca
     1021 agcattaagg tctgataaag aggggttaac tcctgaaaaa ttattagtct tgcaggatca
     1081 tgtgcttaat tataatgtcg aggtttcatt ggtggaacat tagcaagaaa gattgtcgct
     1141 gcagttgaga cgttgacccg ctcatgaaat atatatcatt attattgttt atcctgctgt
     1201 tgtgcggctg caagcagcag gaattattga accatcttga tcaacagcaa gctaatgatg
     1261 tccttgctgt tttacaaaga cataatatta atgctgaaaa gaaggatcaa ggcaaaacag
     1321 gattttcaat atttgtcgaa ccaacggatt ttgcttctgc ggtggattgg ctaaaaatct
     1381 acaatctacc gggtaagcct gatattcaaa tatcgcagat gttccctgca gatgcactcg
     1441 tttcatctcc acgagcggaa aaagcaaggc tatattctgc tattgagcag cggttagagc
     1501 aatctctcaa aattatggat ggcattattt cttcgagagt acatgttagt tacgatgttg
     1561 ataatggcga tagtggtaaa actgct
//
LOCUS       DAJOWB010000326         1519 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJOWB010000326 DAJOWB010000000
VERSION     DAJOWB010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.334"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(288..1490)
                     /locus_tag="OHO85_005034"
     CDS             complement(288..1490)
                     /locus_tag="OHO85_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family efflux MFS transporter"
                     /protein_id="HCQ2674238.1"
                     /translation="MKSQAVIHRKNILFFTIGLSLLTIMGILGVDTYLPSIPDIAKEF
                     RQNIPSMQLSIMVYTLCIGFGQLIFGPLSDSIGRRRIMLSGAIIYCVTTYCLTLSTGL
                     GTFLTIRIIQGLSISITLVTAISAVRDVTRGSVAATLYAIIITIEGVVPIASPLLGGI
                     INDVWGWRAIFLMILGYSVVTLTYVYFNFPETLSHKKRITYSLKSSFLTYKEISKQPR
                     FYLPCLSLGLSFSLIYCYVTASPFILMVKYGYSSTQFGVISSIIGGFLLLGAALSGKL
                     LKKRSAPQILSLWLKLIASFIVLCTPLIIFVHHVNVFILFFIVLMFAGGMFESLYTYL
                     TMSSQRTSLGATSALMGAASLVIPSVIGGIGSFLIERNITIWIVYLLSIILIMIFMLR
                     KYRRFTAA"
BASE COUNT          527 a          274 c          300 g          418 t
ORIGIN      
        1 cggcatggaa gaaatatcgg gtcttgctga tgtcgtgttg gcacttcctt agtgccggat
       61 atcgagtggc cagctgcacc tatagggtaa aaatatagtt ctatatggga gcgctatttt
      121 tacaatataa atgctgaacg gaagaatcta accagttaca aaaaacatag ttatgtaatc
      181 cagttgcctg ggcatgcgtg tgatgttgat agctattact gtatcttccg ttcacttacc
      241 ttattgtgaa cagttatata taatttgatc tgctcttatt cagcctgcta ggctgctgta
      301 aatcttcgat actttcttaa cataaagatc atgattagaa taattgaaag taagtaaaca
      361 atccaaatgg tgatatttcg ttcaattaaa aagctaccaa ttccgccgat cactgaagga
      421 atcaccagag aagcggcccc cattaacgca gatgtggcac ccagactggt acgttggctg
      481 gacatagtta ggtaagtata aagtgattcg aacatacctc cggcaaacat caaaacaatg
      541 aagaataata taaaaacatt gacatgatga acgaatatta ttagcggagt gcacaatacg
      601 ataaatgaag caatgagctt cagccataaa ctcaaaattt gtggtgcaga ccgcttctta
      661 agtagtttac ctgatagtgc tgctcccagc agaaggaatc ctccaataat tgatgagata
      721 acaccaaatt gcgtagatga gtatccatac ttaaccatca aaataaatgg ggaagctgtg
      781 acataacaat aaattagaga gaatgaaagt ccaagagaaa ggcaaggtag ataaaatctt
      841 ggttgcttac ttatctcttt gtacgtaaga aatgatgatt ttagtgaata tgtaatccgt
      901 tttttatgac tgagtgtttc agggaagtta aaatagacat acgttaatgt tacaacagaa
      961 tatcctaata tcattagaaa aattgcacgc catccccata catcattaat tattcctccc
     1021 agcagtggag aagcaatagg cacgacacct tcaatagtta tgataatagc gtacagtgta
     1081 gcagccactg aaccgcgtgt tacatcacga acggcactaa ttgctgtaac cagagttatt
     1141 gatatagaaa gcccctgtat gatgcgtatt gtaagaaacg tacccaatcc ggtcgagagt
     1201 gtgaggcaat atgttgtcac acaataaata atagcacctg aaagcataat tcttcgacga
     1261 ccaatagaat cagataatgg gccaaaaatt agctgtccaa aacctataca aagggtataa
     1321 accatgatag acaattgcat ggacgggatg ttttgtctaa attcttttgc gatgtcagga
     1381 attgaaggca aataagtgtc cacacctaga atgcccataa tagtcagcag cgataggcca
     1441 atagtaaaga aaagtatatt ctttctgtga ataacagctt gtgatttcat tatttatcac
     1501 catgaataaa ttaaaagaa
//
LOCUS       DAJOWB010000327         1505 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJOWB010000327 DAJOWB010000000
VERSION     DAJOWB010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.335"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..673)
                     /gene="tssI"
                     /locus_tag="OHO85_005035"
     CDS             complement(<1..673)
                     /gene="tssI"
                     /locus_tag="OHO85_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCQ2674239.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGMVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSS"
     gene            complement(883..1401)
                     /locus_tag="OHO85_005036"
     CDS             complement(883..1401)
                     /locus_tag="OHO85_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HCQ2674240.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
BASE COUNT          351 a          400 c          407 g          347 t
ORIGIN      
        1 cggaagaagg gcggatttgt gcgctgtagc gaaactggct gatgcagagg gtgctcacct
       61 cggtacgggt gttcggattc caggggatct caaaggactc cggcagataa cgcacggtat
      121 cgcacaggac caggctctgg tcggtacttt tttgcgcgtg ctcctcataa aagaagatgc
      181 cttcctccgc cgccatccgg cacaggaaat cgtaatcagt ctcaccgtac tggacacaaa
      241 actcacggga aggatgtggc tcgctgaaca gcgggctcca ctcggtcacc ccgttttcct
      301 gcaatatcgt gccgaggatg ctttcgatgt cctcattctg gaagatacgg aagttctggc
      361 gcagccctgt gcgccacagc ggtgggcaca ccttcatgct gtacagcatc tggtttttgt
      421 cgttctcccc cagttcaaac caggtcacca tacctttcac ccggcgctgt acgtcatcgc
      481 cctgccatat cgtcaggtag gccattttgt cgaggatctg ctggaattca agggagagaa
      541 actgctggct gaccagagag agatcgaggg aaaaaagcga agagagtgac tggttcagat
      601 gaaaggagac caccgcaaaa gcatccggtg gcaggccgtc cacttccagc gtgaaacgta
      661 atccggttga catagcaacc tccggggtta aggaaaaaca gacagttcgc tgttaaagcg
      721 gctgtgacag tacagtgtta cgcttgcgtt caggcacgct accgccgaag tttccggcag
      781 ccgccatctc ccgtgaacgt aaccccttac tgacaacctt tctttctacg gcgtaataca
      841 aaaacagcat ccggcctgat agccggatgc tgttggctta acttatgctt ccagcggtgc
      901 gcgccagtcg tcggcaccgg aggtgccaga tttgacgtgt tcccattcaa ttttgcggta
      961 ggccagtgaa acttcgagca actgggtgaa ctcgcgtttc gccgggtcct ggcaatgcgg
     1021 catatgcagt ttcatatcga caatcgtcga atcagtcagg cgggtagtga aataatgctc
     1081 ctgtttgcct tccacagagg tacgccacca gtgaagttca gtggttggca gcatttcgcc
     1141 ggaggccagt gcgttgtaca tcagcggaac cgctttgttc agcgccacgg taaaaatgaa
     1201 aggcttgtga gcacgctgac ccgcaggctg accggactga ggatcggtcg ggacagtaac
     1261 gttatgcagg aattcctgca ccagcatctc gtcttcgtgc ccctgcacat agatgttgcc
     1321 gacagaatca gcggtaaatg cacctgcagt aatatttccc tgggtctgac cagtgatgga
     1381 gatataacac ggtgttggca tttgtaaact ccttgttaac gtggttaatt actacttaca
     1441 atgacctgcc gttaatccca gacaggcaga tgcagctcac ttcacggaac cgcatcattt
     1501 ttcag
//
LOCUS       DAJOWB010000328         1462 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJOWB010000328 DAJOWB010000000
VERSION     DAJOWB010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.336"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            109..1344
                     /locus_tag="OHO85_005037"
     CDS             109..1344
                     /locus_tag="OHO85_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEc58 family transposase"
                     /protein_id="HCQ2674241.1"
                     /translation="MPRKKKTPVVTPAIDLPKEFLEKLIPGPMDAAGVEAVFQQLKKA
                     VIERALGAELGLHLADAEGGSGNHRNGRSGKTVLTDEGPLRIDVPRDRTGTFEPQLIP
                     KHERRFAGFDDRIVSMYARGMTVREIQGHLAEMYSVEVSPEFISKVTDEVMAEVTAWQ
                     ARPLEAMYPVVFFDALRVKIREDGVVRNKAVYLALGVLADGTRDILGLWIENTEGAKF
                     WMKVFNDLKTRGVNDILIGVADGLKGFPEALEAVFPATTLQTCIVHLIRNSLDYAGWK
                     DRKALAAAIKPIYTAPTAEAAEAELDAFEAGDWGERFPTVVAAWRRAWTRVVPFFAFP
                     PDVRRVIYTTNAIESVHARLRKIIKTRGHFPSDEAATKLIWLALRNITAEWSRASQHW
                     KAAMNQFAIIYEDRFTLAR"
BASE COUNT          282 a          457 c          499 g          224 t
ORIGIN      
        1 ccccgatctg tgcccgatct gtgcccaaag cggcgcttgc gcggccgctt ccgagggtgt
       61 cagaaatttt gtgtgagagg cataccatga tgctgccaag gagcatgtat gccacgcaag
      121 aagaagacgc cggtggtcac gccggccatt gatctgccga aggaatttct cgagaagctg
      181 atcccgggtc cgatggacgc ggccggggtc gaagccgtgt tccagcagct gaagaaggcc
      241 gtgatcgagc gggcgctggg cgcggagctc ggcctgcacc tggccgacgc cgagggcggg
      301 agcggcaacc accgcaacgg ccgcagcggc aagacggtgc tgaccgacga gggtccgctg
      361 cgcatcgacg tgccgcgcga ccggacgggt acgttcgagc cgcagctgat ccccaagcac
      421 gagcgtcgct tcgccgggtt cgacgatcgg atcgtctcga tgtacgcccg cggcatgacg
      481 gtgcgtgaga tccaggggca cctagccgag atgtacagcg tcgaggtgtc gccggagttc
      541 atcagcaagg tcaccgacga ggtgatggcc gaggtcaccg catggcaggc gcggccgctg
      601 gaagcgatgt atccggtggt gttcttcgac gcgctgcggg tgaagatccg cgaggacggc
      661 gtggtgcgca acaaggcggt gtatctggcg ctgggcgtgc tggccgacgg cacccgcgac
      721 atcctcgggc tgtggatcga gaacaccgag ggcgcgaagt tctggatgaa ggtgttcaac
      781 gacctcaaga cgcgcggcgt caacgacatc ctgatcggcg tggctgacgg cctgaagggc
      841 ttcccggagg cgctggaagc ggtgttcccg gcgaccacgc tgcagacctg catcgtgcac
      901 ctgatccgca acagcctgga ctatgcgggc tggaaggacc gcaaggcgct ggcggcggcg
      961 atcaagccga tctacacggc cccgaccgcc gaggccgcag aggccgagct ggatgcgttc
     1021 gaggccggcg actggggcga gcgcttcccg acggtggtcg ccgcctggcg ccgtgcctgg
     1081 acgcgcgtgg tgccgttctt cgcgttcccg ccggacgtgc gccgggtgat ctacacgacc
     1141 aacgcaatcg agagcgtgca tgcgcgcctg cgcaagatca tcaagacccg cggccacttc
     1201 cccagcgacg aggccgccac caagctgatc tggctggcgc tgcggaacat caccgccgag
     1261 tggagccgcg cgtctcagca ctggaaggcg gcgatgaacc agttcgcgat aatctacgaa
     1321 gaccgattca cgctggctcg ctgaaagaga cttccggact aaaccgcctc acacacaaaa
     1381 aatcggacac gcccccgctt cctacctgcc agactcgcaa aaaagggcgg ttgaacttcc
     1441 tatacccaca aagttagatg ca
//
LOCUS       DAJOWB010000329         1447 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJOWB010000329 DAJOWB010000000
VERSION     DAJOWB010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.337"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            5..619
                     /locus_tag="OHO85_005038"
     CDS             5..619
                     /locus_tag="OHO85_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674242.1"
                     /translation="MFFQIFMAQHICRDAVEIHWANGNIQVIRPVRGISINGEAQGGI
                     RPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTLTALLNVASWLKR
                     KPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVVIETMGYEDSDYC
                     ARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFEKHMYGVFMHLRY"
     gene            898..>1447
                     /locus_tag="OHO85_005039"
     CDS             898..>1447
                     /locus_tag="OHO85_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674243.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSVQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADVADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLI"
BASE COUNT          398 a          314 c          359 g          376 t
ORIGIN      
        1 gcatatgttc tttcagattt ttatggcgca gcacatctgt cgtgatgctg tggaaattca
       61 ctgggcgaac ggtaatattc aggtcatcag acctgtgcgg gggatcagta ttaatggtga
      121 agcgcagggc ggaatacgtc ccccttactg ggttattctt gctttttgcc ggagtgccga
      181 tggcagaatc atctgcagtg aagggtatgc tcatgctctt tatcaactga catgtccggt
      241 gcctgtggac agcaaactgg aacgaaacac gctcactgct cttctgaatg tggccagctg
      301 gcttaaaaga aagccaggta cgccggaatt aagtctggaa aggcccctgt ttgatacaga
      361 agtttatgtt aatggtgaaa agaaatatgt actgccggat ttcattgtta cagcaagggc
      421 tcctgacgga aagacggcca gagtggtcat cgaaacgatg ggatatgaag acagtgatta
      481 ctgcgcgaga aaatccaggc agcataccgg catgaagcag attggtgttc tgcataccga
      541 tccaccgaaa tggctggata acgatcatcc cccttttgag aaacatatgt acggtgtttt
      601 tatgcatctc aggtactgag atattttgtg gctcagttct gtaacttttc ccgtaacatt
      661 gtctgttgtt acgggaaagt ccggtttttg tattgcacca gagaataccc agactgtgat
      721 gctgccacaa cgtcagcagg ctttctgaac ggtttaacac cacttcatgt taatgataat
      781 cactatcatt aaatcttgac atgccatttt ctccttaata aattaatact gtatatgtat
      841 ccatatacgt aagcagttaa ttcatttgtt ttcctcagag gatgaaggag ataccgaatg
      901 tctgaccctg tacgtattac aaatcccggt gcagaatcgc tggggtatga ttcagatggc
      961 catgaaatta tggccgttga tatttatgta aaccctccac gtgtcgatgt ttttcatggt
     1021 accccgcctg catggagttc cttcgggaac aaaaccatct ggggcggaaa cgagtgggtc
     1081 gatgattccc caacccgaag tgatatcgaa aaaagggaca aggaaatcac agcgtacaaa
     1141 aacacgctca gcgtgcagca gaaagagaat gagaataagc gtactgaagc cggaaaacgc
     1201 ctttctgcgg caattgctgc aagggaaaaa gatgaaaaca cactgaaaac actccgtgcc
     1261 ggaaacgcag atgtcgctga tattacacga caggagttca gactcctgca ggcagagctg
     1321 agagaatacg gattccgtac tgaaatcgcc ggatatgatg ccctccggct gcatacagag
     1381 agccggatgc tgtttgctga tgctgattct cttcgtatat ctccccgcga ggccaggtcg
     1441 ttaatcg
//
LOCUS       DAJOWB010000330         1441 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJOWB010000330 DAJOWB010000000
VERSION     DAJOWB010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.338"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..>1441)
                     /locus_tag="OHO85_005040"
     CDS             complement(<1..>1441)
                     /locus_tag="OHO85_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HCQ2674244.1"
                     /translation="AKSSTSLMTESAKTASGNYTVTVSNGSGICGSASLSVEGNGTTD
                     QSTIGKFFNPFSKNEYSGVISAQRAAMDNMISDMDNAASEFVTTFLEKRNSGNGTLPD
                     IETRIQRAADEYERAVQKSLPTDNGEQSRKEALKSYLTTYGWVTLGAWYQTFATANQR
                     LAELADRAPAVTSMSSLGEVGDTDLFSAVMSAYKTQLQNTTYTPPLGTVTSANEQRMA
                     NAQDPKSALSEMMSFFVYRTNWIATEISGTGTTSDQVNPLIKMKNIGDYTLGTTEILW
                     SGYTTVRVLATVGDNSIFGKVANLFSGLPKGLVALLDAAAPPIYFLLFLLFCAGFSLS
                     IYLPFIPFIFWMTGIGNWIVSVLIGCTAGPLWAATHLGTSEDRGSRAAYGYIYLIDSM
                     IRPPIMVFGFFFASVAIIAVGTILNALFGAALVNVQFNSLTGIFSLAGFLLIYARICT
                     TSVAAIFALQAYLPDHVINFLGGREGANTL"
BASE COUNT          394 a          323 c          322 g          402 t
ORIGIN      
        1 caagagtgtt ggctccctca cgcccaccaa ggaaattaat aacatgatca ggtaggtagg
       61 cctgaagggc aaatatggca gctacagagg ttgtacaaat tctggcgtat atcagcaaga
      121 aaccagcaag actgaaaata ccggtcagcg aattgaactg tacgttaacc agggcagcgc
      181 caaataatgc attcagtatt gtgcctacgg cgatgatggc aacagaagca aagaaaaaac
      241 caaagaccat gatgggcggc cttatcatac tatcaattaa gtatatataa ccgtaggctg
      301 cccgacttcc tctatcttct gatgttccga gatgtgttgc agcccacaga ggtccggcgg
      361 tacatcctat taatacactg actatccagt tcccaatgcc tgtcatccag aaaatgaatg
      421 gtataaatgg taggtaaatt gataaggaaa aacccgcaca aaacaacagg aacaacaaga
      481 aatatattgg gggagctgca gcatccagca gagcaactaa tcctttaggt aatccagaga
      541 ataaattggc aacctttccg aaaatagagt tatcacctac tgtcgcgaga actctaactg
      601 ttgtatatcc actccagaga atctctgttg ttcctaatgt ataatcacca atatttttca
      661 tctttatcaa tggattgacc tgatcgcttg ttgttcctgt gccacttatc tctgttgcta
      721 tccagttggt tcgatagaca aagaagctca tcatttcaga taaggcagac ttaggatctt
      781 gcgcattggc cattcgttgc tcattggctg atgtaacagt gcccagaggt ggtgtatagg
      841 tggtgttctg taattgagtc ttgtaggcac tcatgactgc actgaataag tcagtatcac
      901 caacttctcc cagtgatgac attgaagtta cagctggagc tctgtcagcc agctcggcca
      961 gacgctggtt agcagtggca aaggtctgat accatgcgcc aagcgttacc cacccgtagg
     1021 ttgtgagata agatttaaga gcctcttttc tggattgctc tccgttatca gtgggtagcg
     1081 atttttgtac agcacgttca tactcgtcgg ctgctctctg aatccgtgtc tcgatatccg
     1141 gcaatgttcc gttgccagaa tttctttttt ccagaaatgt tgtcacaaac tcactggcag
     1201 cgttgtccat gtcgctaatc atattatcca tggctgcacg ttgtgctgag ataacaccgc
     1261 tgtattcgtt tttactgaag ggattaaaga attttccaat cgtcgattga tctgttgttc
     1321 cgtttccttc tactgacagg ctggctgaac cacagatacc gctgccattg gataccgtga
     1381 cagtgtaatt accactggcg gttttagctg attctgtcat cagtgaagtc gaggatttgg
     1441 c
//
LOCUS       DAJOWB010000331         1439 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJOWB010000331 DAJOWB010000000
VERSION     DAJOWB010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.339"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            253..1368
                     /locus_tag="OHO85_005041"
                     /pseudo
     CDS             253..1368
                     /locus_tag="OHO85_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          545 a          272 c          230 g          392 t
ORIGIN      
        1 cactatcgga gaacatcata actttttaaa atgagcgctt tttgatagtt gttaacgttt
       61 tcttttacga cactatcaat gccctttttt atacacaaat attaccgggt ggagataaaa
      121 gggaagtcaa aggtaattat atcaggaaga tatgacgcat gatatttatt gtgcgtagaa
      181 gaaaatccat tcctgctcta attatccaat ttaaaaatct tagcataaaa cagataatga
      241 ataaggaatg ttatgtcaat tgactttacc ccaggtatga taaatacata tcacggcgat
      301 atttataacc gcacaacaga taccgacaat gttaaaacac cagacacacc gatgtggcct
      361 tgtgataatc gggaagaaca acaacccatt aattccacat tctctggaga aggatacaac
      421 cctgaacaat atgatttagc gcggcaacac ctcccacaaa tttatgcgtg ccgcacgaac
      481 accaaatata cagatgcaga ctactcaaaa gttgtggctc aacttgtaag tcttattact
      541 aacattgaat cgataagttc gacaccactt acgcggcagg cacaggaaat acttaatcaa
      601 attaataaca ttagatacga aaagaacaaa agtgctgaat gtcgaatcat tgttgtcgct
      661 aatcctaaac ccgacaaggc aatcataact aaaattagcg tagaagaggg aataccagta
      721 agattcagtg tccaaaccat gttttcagat actaatttta ttgctgaaca acgagctgac
      781 ttaccgacta atataaaaga tattcaaagc ctgtatcaaa aaatgacgaa gttatatatt
      841 gagcacagtg aaaataaaaa caggatgaaa gtctttgccg gaaccaactt tattgatttc
      901 aatatgacag gacagaacct gtccgggttc gttctaacct tgtcaaggtt ttatttcgaa
      961 gatctactta atataaattt cactgacgca aatttaggtg acactatttt ttacataaag
     1021 aacaccctac ccccaaatta tataaagatg gacaatatct tgacaaacaa atcgaaggtt
     1081 tattttcaac attattaacc attaatgaca actttttacg agcaaaagcg gaaattgctt
     1141 cgacaataat taaattttta gaggccagga taaccaatct ttcatacaat gacatattaa
     1201 catatcagca agaattccaa aaacaatgtt ataagcaagt taaagccttt acgacactct
     1261 ctcgatacaa taaaattcaa acatgggcag aaatgtctga atatcagttc gaagtatttc
     1321 aatacgagac gcttaacccc aaaaaaatgt cacacacgcc ttatttaaag aggccacttc
     1381 ctaatgaaaa agatataaat tatggggtgg aaatagaaat accatctggc aaaagaatt
//
LOCUS       DAJOWB010000332         1436 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJOWB010000332 DAJOWB010000000
VERSION     DAJOWB010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.340"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..356
                     /locus_tag="OHO85_005042"
     CDS             <1..356
                     /locus_tag="OHO85_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCQ2674245.1"
                     /translation="NRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVN
                     SDLRESGAIEQDADLIMFLYRDEVYHPDSEMKGIAEVIIGKQRNGPIGTVRLAFNGQY
                     SRFDNYAGADWQEDY"
     gene            356..1354
                     /locus_tag="OHO85_005043"
     CDS             356..1354
                     /locus_tag="OHO85_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674246.1"
                     /translation="MQWNEEKPMNILIIGRKFAAISDVKTYTEMWSYNLACAFSEAGV
                     TLQYHRPYSPGVESPEDYVEAVLTAATACSAKAILAPGLRYFTTVPREIGMQLCRRFT
                     GWVAQVYDGSMLDSAPVDITFTVRDDTWRYLDNPGRLERHNRFNKHVGWAANQDLFHL
                     ETKTDDVLRIFVDHAAFDVSGFDHSLSILMNLQRLTVPYEARTLTDDGLVTIDPGNIS
                     VTPYRRTPVPATEFAAELRKSDVFIVTHPESLGLTVLEAAMCGALVLTPPDCLPPDRL
                     ALVNHMVIKSRIDWDEVIARVDRVKNAEKVQCHTWSAIAEKMLETFVTQKPSRGNG"
BASE COUNT          375 a          308 c          381 g          372 t
ORIGIN      
        1 ataaccgaac gctggaaatt gccgagattt ctcgctcact gaaggctttg gcgaaggaat
       61 tacaagtacc ggtggtggca ttgtcacaac ttaatcgttc acttgaacag cgtgcggaca
      121 aacgaccggt aaattcagat ttacgtgaat caggagcaat tgagcaggac gcagacctga
      181 ttatgtttct gtatcgcgac gaagtttatc acccggatag cgaaatgaag ggcattgccg
      241 aggtaattat cggaaagcaa cgaaatggcc caattggcac ggtgagattg gcttttaacg
      301 gccaatactc acggtttgat aactatgccg gtgctgactg gcaagaggat tattaatgca
      361 atggaatgag gaaaagccga tgaacatcct gatcattggg cgaaaatttg cagccatcag
      421 tgatgtgaaa acatatacgg aaatgtggtc ttataacctg gcctgcgcct ttagtgaggc
      481 aggggtaaca ttgcaatacc atcgtccata ttcccctggc gtcgaaagcc ccgaggatta
      541 tgttgaagct gtgttgactg ctgcgacagc atgttctgcg aaggccattt tggcaccagg
      601 attgaggtat tttactacgg tacccaggga aataggcatg caactgtgtc gtcgattcac
      661 tggatgggta gcccaggtat acgacggttc tatgctggat tcggcaccag tcgatattac
      721 ttttactgtc cgcgatgata cctggcggta cctggataat ccaggcaggt tagagcgtca
      781 taatcgtttt aacaaacatg ttggatgggc agcgaatcag gatctgttcc atctggaaac
      841 caaaacagac gatgttctgc gtatttttgt agaccacgct gcatttgatg ttagtggttt
      901 tgatcactcc ttaagtatcc ttatgaacct tcagcgtctg accgttccgt atgaggccag
      961 aacgttgact gatgacggat tggttaccat tgatccgggg aatatttcgg taactccata
     1021 cagacggacg ccggtgccag caaccgaatt tgcagctgaa ttgcgtaaga gtgacgtgtt
     1081 tatcgttacg catcccgaaa gccttggatt aactgtactt gaggcggcaa tgtgcggggc
     1141 gttggtatta acgcctcccg attgccttcc gccagatcgc ctggctttgg tgaaccatat
     1201 ggttatcaag tcgcggattg attgggatga ggttattgct cgcgttgatc gcgtgaaaaa
     1261 tgctgaaaag gtccagtgtc acacctggtc ggcaattgcg gaaaagatgc ttgagacgtt
     1321 tgtcacgcag aaaccgtcgc gcggtaacgg ataaaaaatt gaacccgtca taacagaaaa
     1381 gcccgaacgc cgggcttttc ttaagccttg tcaacagaga cttgagcggc ttttat
//
LOCUS       DAJOWB010000333         1430 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJOWB010000333 DAJOWB010000000
VERSION     DAJOWB010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.341"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            52..1112
                     /locus_tag="OHO85_005044"
                     /pseudo
     CDS             52..1112
                     /locus_tag="OHO85_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            1164..>1430
                     /locus_tag="OHO85_005045"
     CDS             1164..>1430
                     /locus_tag="OHO85_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2674247.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTVAELESEILQLRKALNEARLERDILKKATV"
BASE COUNT          436 a          285 c          335 g          374 t
ORIGIN      
        1 acggcactta ttgatatatt catgaagatt ataatcacaa gggaatacat aatgaaaaga
       61 aaagttctgg caatgctggt cccggcgtta ttagttgctg gcgcagcaaa tgcggctgaa
      121 atttataata aagatggcaa taaagttgat ttctacggca agatggttgg cgaacgcatc
      181 tggtcaaata ctgatgataa taacagcgaa aacgaagata cctcctatgc tcgtttcggt
      241 gttaaaggtg aaacccaaat caccagcgaa ctgactggtt ttggtcagtt tgaatacaac
      301 ctcgacgcca gcaagcccga aggtgaaaac caagaaaaaa cccgtttaac cttcgccggt
      361 ttgaaatata acgagttagg atcatttgac tatgccgtaa ctacggtgtt gcttatgatg
      421 ccgcagctta taccgatatg ttagttgaat ggggtggcga ttcctgggct tccgctgaca
      481 acctcatgaa tggtcgtacc aacggtgttg caacctaccg taactatgat ttctttggcc
      541 tggttgatgg tcttgatttt gcgattcagt accaaggcaa aaacagcaac cgtagcacta
      601 agaaacaaaa tggtgacggc tatgcgttgt ctgtagacta taacatcaat ggttttggta
      661 tcgttggtgc atatagcaaa tctgatcgca ccaacgatca agttgccgat ggaaacggtt
      721 caaatgctga actgtggtca ttggctgcaa agtatgacgc gaaaaatgtc tacgcagctg
      781 taatgtacgg cgaaactcgt aatatgactc cgggttctat tgatacaggt gttgccgatc
      841 gagaaggcaa tacaataatg cgtgatcaac ttatcaatga aacccagaac tttgaagcgg
      901 ttgttcagta tcaattcgac tttggtctgc gtccgtcctt aggttatgtg tactctaaag
      961 gtaaagatat caaaggggta cccggacata gatatgttga cgctgaccgt gtgaactaca
     1021 ttgaagttgg tacctggtac tactttaata agaacatgaa cgtttatact gcatacaaat
     1081 tcaacatgct ggataaagat gaggtagcct gagtttaacg gacactcctt cctgaaatag
     1141 aatggcatca gaaggagcta ataatgagca gaaaaaccca acgttactct aaagagttca
     1201 aagccgaagc tgtcagaacg gttcttgaaa atcaactttc gatcagtgaa ggcgcttccc
     1261 gattatctct tcctgaaggc actttaggac aatgggttac cgccgccaga aaagggctcg
     1321 gtactcctgg ttcccgcacg gtggctgaac tggaatctga aattctgcaa ctgcgtaagg
     1381 cgttaaatga agctcgcctt gagcgagata tattaaaaaa agcaactgta
//
LOCUS       DAJOWB010000334         1410 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJOWB010000334 DAJOWB010000000
VERSION     DAJOWB010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.342"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>1410
                     /gene="rhsB"
                     /locus_tag="OHO85_005046"
     CDS             <1..>1410
                     /gene="rhsB"
                     /locus_tag="OHO85_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HCQ2674248.1"
                     /translation="AGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGR
                     VIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYD
                     EADRLTHRTVKGETAERWRYDERGWLTDISHISEGHRVTVHYGYDEKGRLTGERQTVH
                     HPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWLSGMKLGDTPLVEYT
                     RDRLHRETLRSFGRYELTTAYTPAGQLQSQHLNSLLSDRDYTWNDNGELIRISSPRQT
                     RSYSYSTTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARD
                     AHYLYRYDRHGRLTKKTDLIPEGVIRTDDERTHRYHYDSQHRLVHYTRTQYAEPLVES
                     RYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRLTTIQNDRSRIQTIY
                     QPGSFTPLIRVETATGELA"
BASE COUNT          334 a          454 c          417 g          205 t
ORIGIN      
        1 ccgccggtga cctgaccacc gtcattgccc cggacggcag cagaaacggg acacagtacg
       61 atgcgtgggg aaaagccatc tgtaccacgc agggcggtct gacgcgcagt atggaatacg
      121 atgctgccgg acgggtcatc cgcctgacca gtgaaaacgg cagccacacc accttccgtt
      181 acgatgtact cgaccggctg atacaggaaa ccggctttga cggccgcaca cagcgttatc
      241 accacgacct gaccggcaaa cttatccgca gcgaggatga ggggctggtc acccactggc
      301 actatgacga agcagaccgc ctcacgcacc gcaccgtgaa gggtgaaacc gcagagcggt
      361 ggcggtacga cgaacgcggc tggctgacag acatcagcca tatcagcgaa gggcaccggg
      421 tgacggtgca ttacgggtat gatgagaaag gccgcctgac cggtgagcgt cagacggtgc
      481 atcacccgca gacggaagca ctgctctggc agcatgagac cagacatgcg tacaacgcgc
      541 aggggctggc gaaccgctgt ataccggaca gcctgcccgc cgtggaatgg ctgacctacg
      601 gcagcggctg gctgtcaggc atgaaactcg gcgacacacc gctggtggag tacacccgcg
      661 accgcctgca ccgggaaacg ctgcgcagct tcggtcgtta tgaactcacc accgcttaca
      721 cccctgccgg gcagttacag agccagcacc tgaacagcct gctgtctgac cgcgattaca
      781 cctggaacga caacggcgaa ctcatccgca tcagcagccc gcgccagacc cggagttaca
      841 gctacagcac caccggcagg ctgaccggcg ttcacaccac cgcagcgaac cttgatatcc
      901 gcatcccgta tgccacagac ccggcaggta accgcctgcc cgacccggag ctgcacccgg
      961 acagcaccct cagcatgtgg ccggataacc gtatcgcccg tgacgcgcac tatctttacc
     1021 ggtatgaccg tcacggcagg ctgacaaaga aaaccgacct catcccggaa ggggttatcc
     1081 gcacggatga tgagcggact caccggtacc attacgacag tcagcaccgg ctggtgcact
     1141 acacgcggac acaatatgca gagccgctgg tcgaaagtcg ctatctttac gacccgctgg
     1201 gccgcagggt ggcaaaacgg gtatggcgac gtgaacggga cctgacgggc tggatgtcgc
     1261 tgtcacggaa accgcaagtg acctggtacg gctgggacgg cgaccggctg accacgatac
     1321 agaacgacag gagccgcatc cagacgattt atcagccggg gagcttcaca ccactcatca
     1381 gagttgaaac tgccaccggt gaactggcca
//
LOCUS       DAJOWB010000335         1408 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJOWB010000335 DAJOWB010000000
VERSION     DAJOWB010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.343"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>1408
                     /locus_tag="OHO85_005047"
     CDS             <1..>1408
                     /locus_tag="OHO85_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCQ2674249.1"
                     /translation="QQVLAAQQAAIKEAEQRSVADKATADAKAKALAQQEAQQYSDKQ
                     ALQGRLQAAPKYQHAAREKAASQIANPGTARYKQFDDNPVKQVAQNPLATFSLDVDTG
                     SYANVRRFLNHGLLPPPDAVRVEEIVNYFPYDWDIKDKQSIPATKPIPFAMRYELAPA
                     PWNEQLTLLKIDILAKDHKSEELPASNLVFLIDTSGSMISDERLPLIQSSLKLLVKEL
                     REQDNIAIVTYAGDSRIALPSISGSHKAEINAAIDSLDAEGSTNGGAGLELAYQQAAK
                     GFIKGGINRILLATDGDFNVGIDDPKSIESMVKKQRESGVTLSTFGVGNSNYNEAMMV
                     RIADVGNGNYSYIDTLSEAQKVLNSEMRQTLITVAKDVKAQIEFNPAWVTEYRQIGYE
                     KRQLRAEDFNNDNVDAGDIGAGKHITLLFELTLNGQKASIDKLRYAPDNKSVKSDKTK
                     ELAWLKIRWKYPQGKESQL"
BASE COUNT          418 a          317 c          354 g          319 t
ORIGIN      
        1 ccagcaagtg cttgccgcgc aacaagctgc aataaaagag gccgagcaaa gatccgtcgc
       61 cgataaagcc actgcagacg cgaaagcaaa agccttagcc cagcaagaag cgcaacaata
      121 ttcagacaaa caggctttgc aggggcgctt acaggcggct ccaaaatatc aacatgcagc
      181 cagagaaaaa gcagcgtccc aaatcgcaaa tccaggaacc gctcgctaca agcagttcga
      241 tgataatccg gttaagcagg tagcgcaaaa cccgttggcg acgtttagcc ttgacgtcga
      301 caccggcagt tatgcgaatg tacgccgttt tctcaatcat gggctgttac ctccgccaga
      361 cgctgtgcgg gtggaggaga tagtcaatta tttcccgtat gactgggata tcaaagacaa
      421 acaatctatt ccggccacta agccaatacc tttcgctatg cgctacgaac tggcacctgc
      481 gccgtggaat gaacagctaa cattgctgaa aattgatatc ctggcgaaag atcataaaag
      541 tgaagagtta ccagcttcta atctggtctt tcttatcgac acttctggtt caatgatttc
      601 tgatgaacgt ttgccactta tccagtcttc gttgaaatta ttggtcaaag aacttcgtga
      661 gcaggataac attgccatcg tgacctacgc tggcgactcc cgtattgcgt tgccttctat
      721 ctccggtagt cacaaggcgg aaattaatgc cgcaattgat tcgctggatg ccgaaggcag
      781 taccaatggc ggtgccgggc tggaactggc ttatcagcag gcggcgaagg ggtttattaa
      841 gggcggcatc aatcgcattt tattagccac tgacggtgac tttaacgttg gcattgacga
      901 tccaaaatcg attgaatcaa tggtcaaaaa acagcgggag tctggtgtta ctctgtcgac
      961 gtttggcgtg gggaatagca attacaacga ggcaatgatg gtgcgaattg ccgatgttgg
     1021 taacggcaac tacagctaca ttgataccct ctctgaagcg cagaaagtat tgaatagtga
     1081 aatgcggcag acgttgatta ccgtagcaaa agatgtcaaa gcgcaaattg agtttaaccc
     1141 cgcgtgggta acggaatacc gtcagattgg ttatgaaaag cgccaacttc gggcggaaga
     1201 ttttaataac gacaacgttg atgcggggga tattggcgca ggcaaacata taacgttgtt
     1261 attcgaatta acgctgaacg ggcaaaaagc atcaattgat aagttacgct atgccccgga
     1321 taacaaatca gtgaaatcgg acaaaacgaa agaactggcc tggttaaaga ttcgctggaa
     1381 atacccgcag ggaaaagaaa gtcagtta
//
LOCUS       DAJOWB010000336         1392 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJOWB010000336 DAJOWB010000000
VERSION     DAJOWB010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.344"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..97
                     /locus_tag="OHO85_005048"
     CDS             <1..97
                     /locus_tag="OHO85_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HCQ2674250.1"
                     /translation="IRLTPQLTGKGQQWLTRKLLDAGLLKPVAIG"
     gene            261..1061
                     /locus_tag="OHO85_005049"
     CDS             261..1061
                     /locus_tag="OHO85_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001187871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCQ2674251.1"
                     /translation="MQQTFNADMNISNLHQNVDPSTTLPVICGVEITTDRAGRYNLNA
                     LHRASGLGAHKAPAQWLRTLSAKQLIEELEKETMQNCIVSFEGRGGGTFAHELLAVEY
                     AGWISPAFRLKVNQTFIDYRAGRLQPAIPQSLPEALRLAADLAEQKQRLEQKMLMDAP
                     KVEFAERVATASGVLIGNYAKVLGLGQNYLFTWLRDNGILIATGERRNVPKQEYISRG
                     YFTLKETVIDTSNGSRISFTTRITGKGQQWLMKRLLDAGVLVPVAATR"
     gene            1091..>1392
                     /locus_tag="OHO85_005050"
     CDS             1091..>1392
                     /locus_tag="OHO85_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674252.1"
                     /translation="MLAKVTFLSCITMSDFTFSGYELACFVTHSGLSRSAGHILSQCA
                     NLAATTSEYFIHKPHRLIAAETGYSQSTVVRAFREAVNKGILSVEIVIGDHRERRA"
BASE COUNT          391 a          338 c          331 g          332 t
ORIGIN      
        1 gatacggctg acgccccaat taacgggtaa aggccagcag tggttaactc gcaagctact
       61 tgatgctggt ttgttaaaac cagtagcaat aggttaacaa aagaaaaaac ctgccagcaa
      121 actggcaggt ttctgagcag atcgaccaac ccgatctgga tcgagtcaga aaaatttgct
      181 ctaataaatt tcgttttcta agtgcaaaga atcaccattt cgagctggtg attgaaggtt
      241 gatgcaaatt tggagaaaaa atgcaacaaa cattcaatgc ggatatgaat atatcaaacc
      301 ttcatcaaaa tgtcgatcct tcaaccactc tgcctgttat ttgtggtgtt gaaattacga
      361 ccgaccgcgc tggccgttac aaccttaatg ctctacacag agcgagcgga ctcggtgccc
      421 ataaagcgcc agctcaatgg ctaagaacgc tgtcagccaa acagctcatc gaagagcttg
      481 aaaaagaaac tatgcagaat tgcatagttt cgttcgaagg ccgtggcggc ggcacttttg
      541 cccatgaatt gctcgcagtg gagtacgcag gttggatttc tcccgcgttt cggctgaagg
      601 taaaccagac atttatcgac tatcgagctg gaagattaca acctgctatt ccgcagagcc
      661 tcccagaagc tctccgtttg gctgctgacc tggcagagca aaagcaacgg ctggagcaaa
      721 aaatgctgat ggatgcacct aaagtcgaat tcgccgaacg cgttgctacc gccagcgggg
      781 ttctaatcgg caactatgcc aaagtgctcg gcctgggcca aaactatctc ttcacctggt
      841 tgcgtgataa cggaattctg atcgcaaccg gtgaacgcag gaacgtcccc aaacaagaat
      901 acatatcccg tgggtatttc acccttaaag aaaccgtgat cgatacaagc aatggaagca
      961 ggatttcttt cacgactcgt ataaccggca aaggtcagca gtggctgatg aagcgattgc
     1021 ttgatgctgg tgtgctggta cctgtcgcgg caacgcgcta acagacgtag taagaaccac
     1081 cagcattgta atgctggcta aagtcacttt cctgagctgt ataacgatga gcgattttac
     1141 tttttctggc tatgaattgg cctgctttgt aacacactcc ggtctatccc gtagcgccgg
     1201 gcatatcctg tcgcaatgtg caaatctagc ggcaacaacc agtgaatact tcattcacaa
     1261 gcctcaccgc ctgatcgcgg cagaaactgg ttatagccaa tcaaccgtcg ttcgtgcatt
     1321 ccgtgaagct gtaaacaaag gaattctgtc tgtagagatt gttatcggcg atcaccgtga
     1381 acgtcgcgct aa
//
LOCUS       DAJOWB010000337         1387 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJOWB010000337 DAJOWB010000000
VERSION     DAJOWB010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.346"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..872
                     /locus_tag="OHO85_005051"
     CDS             <1..872
                     /locus_tag="OHO85_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCQ2674253.1"
                     /translation="FINWMRDCHSSDDKDVIAIDGKTLRHSYDKSRRRGAIHVISAFS
                     TMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDY
                     LFAVKGNQGRLNKAFEEKFPLKELNNPAHDSSAMSEKSHGREEIRLHIVCDVPDELID
                     FTFEWKGLKKLCVAVSFRSIIAEQKKEPEMMVRYYISSADLTAEKFATAIRNHWHVEN
                     NLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINVLTNDKVFKAGLRRKMRKAAMDR
                     NYLASVLAGSGLS"
     gene            902..1315
                     /locus_tag="OHO85_005052"
     CDS             902..1315
                     /locus_tag="OHO85_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674254.1"
                     /translation="MLVGAAVGAVVIGGGIVIYNNREAVGDAIEKSMEMRGEAELLNA
                     QSQMEFWREPYGAFSSVFGNDDSSKENPNVGKDLTDEEKNCLGSGSTGGSGGWEPDED
                     GEIRNTYRSIKDAPQYPRGVRNVQNGTTRNVVKDQ"
BASE COUNT          443 a          224 c          380 g          340 t
ORIGIN      
        1 gctttattaa ctggatgcgt gactgccatt cttcagatga taaagacgtc attgcaattg
       61 atggaaaaac gctccggcac tcttatgaca agagtcgccg caggggagcg attcatgtca
      121 ttagtgcgtt ctcaacaatg cacagtctgg tcatcggaca gatcaagacg gatgagaaat
      181 ctaatgagat tacagctatc ccagaacttc ttaacatgtt ggatattaaa gggaaaatca
      241 tcacaactga tgcgatgggt tgccagaaag atattgcaga gaagatacaa aaacagggag
      301 gtgattattt attcgcggta aaaggaaatc aggggcggct aaataaagcc tttgaggaaa
      361 aatttccgct gaaagaatta aataatccag cgcatgacag ttccgcaatg agtgaaaaga
      421 gtcacggcag agaagaaatc cgtcttcata ttgtttgcga tgtccctgat gaacttattg
      481 atttcacgtt tgaatggaaa gggctgaaaa aattatgcgt ggcagtctcc tttcgctcaa
      541 taatagcaga gcaaaagaaa gagcccgaaa tgatggtcag atattatatc agttctgctg
      601 atttaaccgc tgagaagttt gcgacagcga ttcgaaatca ctggcacgtg gagaataatc
      661 tgcactggcg tctggacgtg gtaatgaatg aagacgactg caaaataaga agaggaaatg
      721 cagcagaatt attttcaggg atacggcaca tcgctattaa tgttttgacg aatgataagg
      781 tattcaaggc agggttaaga cgtaagatgc gaaaagcagc catggacaga aactatctgg
      841 cgtcagtcct tgcggggagc gggctttcgt aatcttgccc tgggtaggag gccctccagg
      901 gatgttagtc ggtgcggcag ttggagcggt ggttattggt ggtggtatag taatatacaa
      961 caatcgcgag gctgttgggg atgcgattga gaaaagtatg gagatgcggg gtgaagccga
     1021 attattaaat gcacaaagtc aaatggaatt ctggcgagaa ccctatggtg ctttttccag
     1081 cgtgtttgga aatgatgatt catcaaaaga gaatccaaat gtcgggaagg atttgacgga
     1141 tgaagagaag aactgtttgg gcagtggcag tacaggaggt tcgggagggt gggagccaga
     1201 tgaggatggc gaaataagaa atacctacag gtctattaag gatgctcctc agtatcccag
     1261 aggggttagg aatgtccaaa atggcactac aaggaatgtt gtaaaggatc agtagttatt
     1321 gaaacaatta cgtaaagtag attcaggaaa atagagtaaa gtttataaag atggatttga
     1381 tgcgtca
//
LOCUS       DAJOWB010000338         1381 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJOWB010000338 DAJOWB010000000
VERSION     DAJOWB010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.347"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>1381
                     /locus_tag="OHO85_005053"
     CDS             <1..>1381
                     /locus_tag="OHO85_005053"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674255.1"
                     /translation="HFLITDKYADDGLNNALKVIISYLVPAGRYVSFHTDKTVVETLS
                     NYNLQPEVYLFYEHGLLKTQIHQGEEITLSPELCVFMKSPLARSELPYQETIYGYSHP
                     PKNLLAFARDYTNPWLIRGIVEFPFRNRSTYHLQQYSHQILEHSAPDSPDYAAALAVL
                     GYQMLSGSDDTADIYAKMLDYCSNVSQMDNPTPHQYRWLISLSTLLGLICNKNNDKAN
                     ALIHLSRAANSSIDKFSPSIGTKILQSFYLQSVILISLNRISCAEIIVDRGIKRGIQL
                     LYQHPDELVGKISQPFNFVLYIYHDILDWLIKMVNIKNAIPGRKFNIANFDNGNTWSA
                     LLHERMNAINNMSQMIDERDRTIHDQKCLIDERDRTIHDQKRLIDERDSTVLTQKNLI
                     DERDLVSAQQNQLIEQNNKTIQQLIQNVTDLNSQVSSKEQKVDELQNQNIKLISLIDE
                     KDLHIAQLST"
BASE COUNT          459 a          269 c          240 g          413 t
ORIGIN      
        1 ccattttctc attactgata aatatgccga tgatggacta aataatgcac tcaaagtcat
       61 tatatcttac ctggtgcccg ctggcaggta cgttagtttt catacagata aaactgtagt
      121 tgagacatta agtaattaca atttacaacc tgaagtttat ttattttatg aacatggcct
      181 tttaaagact caaatacatc agggagagga aattacactc tctcctgaat tatgtgtttt
      241 tatgaaaagc cctttagcca ggtcagagct tccgtatcag gaaacgattt atggctactc
      301 gcatccacca aaaaacctat tagcttttgc cagagattat actaacccgt ggcttattcg
      361 cgggatcgta gaattcccat tcaggaatag gtcaacttat catctacagc agtatagcca
      421 tcagattttg gagcacagtg ctcctgacag ccccgattat gccgctgcat tggcagtatt
      481 aggttatcaa atgctaagcg gcagtgatga tacagctgac atttacgcaa aaatgctaga
      541 ttactgctca aatgtgtcgc agatggataa tcctacgcca catcaatatc gttggctcat
      601 atctttatcg acacttttag gtcttatttg taacaaaaat aatgataaag ctaatgcgtt
      661 aatccattta tctcgtgcag caaactccag cattgataaa tttagcccct ctatcggcac
      721 gaagatttta caaagctttt atcttcagtc cgttatatta atttctttaa atagaatctc
      781 ctgtgccgag ataatcgtcg atagagggat taaaagaggt atacagctac tttatcaaca
      841 tcctgacgaa ctggtgggta aaatatctca accatttaat tttgtgcttt atatttacca
      901 tgatattctt gattggctta taaagatggt caacattaaa aacgccatac caggtagaaa
      961 attcaacatt gcaaattttg acaacggtaa cacctggtcg gctttgcttc atgaacgcat
     1021 gaatgcaata aataatatgt cgcaaatgat agatgagcgt gacagaacaa ttcatgatca
     1081 aaaatgctta attgatgaac gcgatagaac tattcacgac cagaagcgcc ttattgatga
     1141 gcgagacagc actgttctta cgcaaaaaaa tttaattgat gagcgtgacc tggtctctgc
     1201 tcagcaaaat caattaattg agcagaataa taaaactatt cagcagctaa ttcaaaatgt
     1261 aactgactta aatagtcaag tttcctctaa ggaacagaaa gttgatgaat tacagaatca
     1321 aaacataaaa ttaatttcat taatcgatga aaaagatctc catattgccc agctctctac
     1381 a
//
LOCUS       DAJOWB010000339         1378 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJOWB010000339 DAJOWB010000000
VERSION     DAJOWB010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.348"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..173)
                     /locus_tag="OHO85_005054"
     CDS             complement(<1..173)
                     /locus_tag="OHO85_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCQ2674256.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLP"
     gene            complement(208..405)
                     /locus_tag="OHO85_005055"
     CDS             complement(208..405)
                     /locus_tag="OHO85_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674257.1"
                     /translation="MQGAPHTARLKKNDGTTMACGGHWHTTPAQVKTEKTARHLQAKK
                     VKFLHFFTLIYRADLAIVMIK"
     gene            754..>1378
                     /locus_tag="OHO85_005056"
     CDS             754..>1378
                     /locus_tag="OHO85_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCQ2674258.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRRIDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNACKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLL"
BASE COUNT          313 a          366 c          365 g          334 t
ORIGIN      
        1 ggcagcaggt cacggactga aagtgcctgg gtggtcctcc agacggtgac gtccacctgt
       61 tccgcatttt cccaggcgtg ccatgttctc tgcgtcacgc caaattctct ggccgctttt
      121 tcgcgtgacc agagcatgct ttttcgccag attcgcagtt cccagccggt cataaaacac
      181 ctttgaaaaa gtgaaaaaaa cttcacttta ttttatcatc acaattgcca aatcagcgcg
      241 ataaataagt gtaaaaaagt gaagaaattt cacttttttt gcttgtaaat gtcgtgcggt
      301 tttctctgtt ttcacctgtg cgggagtggt gtgccagtga ccaccacatg ccattgttgt
      361 gccgtcgttt ttcttcagtc gcgccgtatg cggcgctccc tgcacacctt acggtgtgta
      421 aggccactgc gcctgatggc gcagctgtct ctgcctgtct gccgccgcac tgcggcctgt
      481 tctccgcaga cggactttcc gggggtacat acatgaccac atcccgtttc agtcgtccag
      541 gggggagatg taccagccac aaaacttttt gccgcaggcg tcaaaaactt ttgcgtctgc
      601 ccccggacaa ctgccccgtg acgtggtgag cgtcccccgt cggaaaggtc cactgcgggg
      661 aaatccccga agacccggac ggcagaagga gcttcgctcc tccgggaaat gacctggctg
      721 tcccggcagt cgtgacgatg aggagacaat gtgatgaatc agactttacc cactgctgac
      781 ctgaatactg ccggcacgac agatgttatt ccgtctgtgg ctatcgaccg catcatcgcg
      841 cagcgtaacg aaggtattgc actgttcatg caggcgatgg aatgcctggc gacagcgcgc
      901 aagattctgc tcgatgcgtc aggtgatatt tttctttacg ggtttgaaga ctgcgtgact
      961 gattccgttc gtcgcataga taaaccggaa gaagcgaaaa ggaatatcac ccgtcttgcc
     1021 gaccggaaaa tctgggaccg cctgatgaca gatacgggca tgtacacctt catgagttca
     1081 tgccagcgtg atgagtggaa cagccagctg atgagcgaca cctgtcctga aatcaccctg
     1141 gacaatgtac tggcaacatt ccgccatctg aatgcctgca agatgcagac atttgaacag
     1201 ggactgattg atgtctaccg gaaattgtca tgggattaca gaaccaataa tccctgccgt
     1261 ctgggtaaga aaatcattat tgaaaacctg ctgtaccgct ggagtaacgg gcgtgtgacg
     1321 ctggactgca gcggacggga ggcactggat gacctggtac gtccgtttta tctgctgg
//
LOCUS       DAJOWB010000340         1370 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJOWB010000340 DAJOWB010000000
VERSION     DAJOWB010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.349"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..573
                     /gene="istB"
                     /locus_tag="OHO85_005057"
     CDS             <1..573
                     /gene="istB"
                     /locus_tag="OHO85_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HCQ2674259.1"
                     /translation="NAGLKYQATPEGLRYPASRGLRAEQMRELLNGHYITHRKNLLIT
                     GPTGCGKSWIANALGEQACRQKHSVRYCRTGRLLEQLAQGRVDGSWLKYLKQLQKIQV
                     LILDDLGLEQLSNAQCNDLLELIEDRYGQSSTIVVSQFPVDKWHGLMENPTTADAILD
                     RLVHNSHRVVLQGESLRKNPPVVESSEKTR"
     gene            complement(570..671)
                     /locus_tag="OHO85_005058"
                     /pseudo
     CDS             complement(570..671)
                     /locus_tag="OHO85_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pcar domain protein"
     gene            complement(669..1370)
                     /locus_tag="OHO85_005059"
                     /pseudo
     CDS             complement(669..1370)
                     /locus_tag="OHO85_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
BASE COUNT          397 a          322 c          353 g          298 t
ORIGIN      
        1 aatgccggcc tgaaatacca ggcaacaccg gaagggctgc gctatccggc ctcaagaggt
       61 ctgcgggctg aacagatgag agaactgctg aacggccact acataaccca caggaaaaac
      121 ctgctgataa ccggtccgac aggatgtggc aagagctgga tagccaatgc gctgggcgaa
      181 caggcatgtc gacagaaaca cagcgtccgg tactgtagaa ccgggcgatt actggaacaa
      241 ctggctcagg gacgtgttga tggcagctgg ctgaaatatc tgaaacagtt gcaaaaaata
      301 caggttctaa tcctggacga tctggggctg gaacagttaa gtaacgccca gtgtaatgac
      361 cttctggagc tcatagagga tcgttacgga caaagcagca cgatagtggt aagccagttc
      421 ccggtagata aatggcatgg tctgatggag aacccaacaa cagcagatgc catcctggac
      481 agactggtac ataactcaca ccgggtggtc cttcagggag agtcactgag gaaaaatccc
      541 ccggtagtgg aaagcagcga aaaaacgcgt taaaaagcgg tagaaaaagt taagttcccg
      601 gttgatcgaa tggatgcgaa tcgctgatcg gacttcgatg ccaatccgtg atcgaataga
      661 gcggaatacg cagtatccag agagccggag aagttatgcc gcacagcaag tggaaatgga
      721 tgcaaatttc cgcacagcat ttccgcgatg taagtgtttg tagcaaagcc atgacgaaaa
      781 gctgtggcag ccttaacatg ctccccattc agaaaatgca gaagaccaag ttcataccag
      841 taaggtgaaa attcatcagc atgctctttc agtacactga aggcttgctt atgatcgcct
      901 gttcgtaaca gttctgagcc caacaaccag cgggcaccct gattatcttc aggattgtat
      961 gccagtattt tatcagtaag cataaccgca tcctgatgac gctgtaagtg cacatttgcg
     1021 agaattgctg cataaagtgc gcgcagataa ggccggtttt cgggatgcat ccagatgatt
     1081 tcaccactga aactttccgg gatgagacga ttacctgccg ccagtccttt aagagcggcg
     1141 ttcagtgcct tacgcggggc attctgctcc agaaatgcat atgccagatg agcgtgtata
     1201 tcaataaagt caggctcctg ttgagcaagg cgtctgagtt tgttgatata acccgtctca
     1261 ctgatttcat cagtgtcgta tgcatcaagc acttcttcaa actcccccag taatgggcca
     1321 aaccaggtat ccggaaatac gaaacgaccc tggttattgt tcacttcaaa
//
LOCUS       DAJOWB010000341         1365 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJOWB010000341 DAJOWB010000000
VERSION     DAJOWB010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.350"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..300)
                     /locus_tag="OHO85_005060"
     CDS             complement(<1..300)
                     /locus_tag="OHO85_005060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674260.1"
                     /translation="MLASSSATADAISSARFAAVSARVAADSAVLLLCAAAAALPAAS
                     VAFVDAVVALPFAADACLVASSFEADADDADDAAELADDAAAVFEDSALVSDAFAF"
     gene            278..>1365
                     /locus_tag="OHO85_005061"
     CDS             278..>1365
                     /locus_tag="OHO85_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005033136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCQ2674261.1"
                     /translation="MALEDASTTKKGVVQLSSATNSTSETLAATPKAVKAAYDNAEKR
                     LQKDQNGADIPDKDRFLSNINVYSKGEVDQKKGMRYVVVNAPAGVQEGKYYPLVIKRN
                     DSHRASRVVISTPSRSANHRMNNCEFNGFVCAGGWTDRGSYACGMFWAYSTSERAIHS
                     ILMSNKGDTVDSVFYIEGGAFPVEVFLEEGLSVTAPASDYIVAETTYKFGATDPYSES
                     VAVNLILDFKQGNGFYSSYPVLSKSDISGNKIYANDEVIVRSQNALRMIAGDYGVIWR
                     NDGANTYLLMTDKGDQYGGWNGLRPFAVNNATGEVTINTPLNSPKGIKGNSDTATKLQ
                     TAIKISGVSFDGSTDITLTAAHVAAFAR"
BASE COUNT          391 a          289 c          364 g          321 t
ORIGIN      
        1 gaacgcgaaa gcgtcggaaa ccagcgcaga atcctcaaaa acggctgccg catcgtccgc
       61 cagttcggcg gcgtcatcgg catcatcggc gtctgcttca aaagatgagg cgaccagaca
      121 agcatccgca gcgaagggca gcgccacgac agcatccacg aaggcgacag aggctgctgg
      181 cagtgcggca gcggcagcac agagcaaaag tacggcagaa tccgcagcaa cgcgcgctga
      241 gacagcggca aaacgtgcag aggatattgc atccgccgtg gcgcttgagg atgcgagcac
      301 gacgaaaaag ggggtagtac agctcagcag tgcgaccaac agcacgtctg aaacgctggc
      361 ggcaacgcca aaggcagtaa aagcagccta tgacaatgca gagaaacgtc tgcagaaaga
      421 ccagaacggt gctgatattc ccgacaagga ccgcttcctg agtaacatta atgtttacag
      481 caaaggtgaa gtggatcaga aaaaggggat gcgatacgtt gtggtaaatg ccccggcggg
      541 tgtccaggaa ggtaaatatt atccgttagt gataaaaagg aatgacagcc atcgggcatc
      601 ccgcgttgtc atttcaacgc caagtcgctc agctaatcac agaatgaata attgtgagtt
      661 taatggtttt gtttgcgcag gcggctggac ggaccggggt agctatgcct gtggtatgtt
      721 ctgggcttat tcaacatcgg aacgcgctat tcattccatt ctgatgagta ataaaggcga
      781 taccgtagac agcgtattct atatagaagg tggggcattt cccgtagagg tctttttaga
      841 agaaggtctc tccgttactg cacctgcttc tgattatatt gttgctgaaa cgacttataa
      901 atttggcgca actgatcctt attctgaatc agttgcggta aatttaattt tagattttaa
      961 acaaggtaat ggattttata gttcgtatcc tgttttgagt aagtcagaca ttagcgggaa
     1021 taagatttac gctaatgatg aagttattgt ccgtagtcag aacgcgttaa ggatgattgc
     1081 aggtgattac ggtgtcatat ggcgaaatga tggagcaaat acttacttgc tcatgactga
     1141 taaaggcgac cagtacggtg gctggaacgg tcttcgacca tttgcagtaa ataacgcaac
     1201 aggtgaagtg acgatcaata caccacttaa ttctcctaag gggattaagg gaaattctga
     1261 tacagctacg aaacttcaga cagcaataaa aatatctggt gtttcgtttg atggttctac
     1321 tgatatcact ttaaccgctg cgcatgttgc tgcttttgcc agaag
//
LOCUS       DAJOWB010000342         1343 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJOWB010000342 DAJOWB010000000
VERSION     DAJOWB010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.351"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..229)
                     /locus_tag="OHO85_005062"
     CDS             complement(<1..229)
                     /locus_tag="OHO85_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674262.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAE"
     gene            complement(357..1196)
                     /gene="sul1"
                     /locus_tag="OHO85_005063"
     CDS             complement(357..1196)
                     /gene="sul1"
                     /locus_tag="OHO85_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HCQ2674263.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            complement(1190..>1343)
                     /locus_tag="OHO85_005064"
     CDS             complement(1190..>1343)
                     /locus_tag="OHO85_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="QacE family quaternary ammonium compound efflux
                     SMR transporter"
                     /protein_id="HCQ2674264.1"
                     /translation="LGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLARSPSWKSL
                     RRPTPW"
BASE COUNT          273 a          425 c          410 g          235 t
ORIGIN      
        1 cctcggccga caaggtgcaa cagccgacga tgccgtcgct gcaactcgcg actaggagct
       61 cggatctcag gacgaaggtc tccgcgaatg tccggtcgat ccgcgcgacg tcccaggcgg
      121 gcgttccctt ggcggacatc cacgccgcag cgtcgtgcat cagccgcaca acctcgtcga
      181 tatcacccga gcaggcgacc cgaacgttcg gaggctcctc gctgtccatt cgctcccctg
      241 gcgcggtatg aaccgccgcc tcatagtgca gtttgatcct gacgagccca gcatgtctgc
      301 gcccaccttc gcggaacctg accagggtcc gctagcgggc ggccggaagg tgaatgctag
      361 gcatgatcta accctcggtc tctggcgtcg cgactgcgaa atttcgcgag ggtttccgag
      421 aaggtgattg cgcttcgcag atctccaggc gcgtgggtgc ggacgtagtc agcgccattg
      481 ccgatcgcgt gaagttccgc cgcaaggctc gctggaccca gatcctttac aggaaggcca
      541 acggtggcgc ccaagaagga tttccgcgac accgagacca atagcggaag ccccaacgcc
      601 gacttcagct tttgaaggtt cgacagcacg tgcagcgatg tttccggtgc ggggctcaag
      661 aaaaatccca tccccggatc gaggatgagc cggtcggcag cgaccccgct ccgtcgcaag
      721 gcggaaaccc gcgcctcgaa gaaccgcaca atctcgtcga gcgcgtcttc gggtcgaagg
      781 tgaccggtgc gggtggcgat gccatcccgc tgcgctgagt gcataaccac cagcctgcag
      841 tccgcctcag caatatcggg atagagcgca gggtcaggaa atccttggat atcgttcagg
      901 tagcccacgc cgcgcttgag cgcatagcgc tgggtttccg gttggaagct gtcgattgaa
      961 acacggtgca tctgatcgga cagggcgtct aagagcggcg caatacgtct gatctcatcg
     1021 gccggcgata caggcctcgc gtccggatgg ctggcggccg gtccgacatc cacgacgtct
     1081 gatccgactc gcagcatttc gatcgccgcg gtgacagcgc cggcggggtc tagccgccgg
     1141 ctctcatcga agaaggagtc ctcggtgaga ttcagaatgc cgaacaccgt caccatggcg
     1201 tcggcctccg cagcgacttc cacgatgggg atcgggcgag caaaaaggca gcaattatga
     1261 gccccatacc tacaaagccc cacgcatcaa gcttttgccc atgaagcaac caggcaatgg
     1321 ctgtaattat gacgacgccg agt
//
LOCUS       DAJOWB010000343         1342 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJOWB010000343 DAJOWB010000000
VERSION     DAJOWB010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.352"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1136
                     /gene="merA"
                     /locus_tag="OHO85_005065"
     CDS             <1..1136
                     /gene="merA"
                     /locus_tag="OHO85_005065"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HCQ2674265.1"
                     /translation="LRHAKYEGILEGNPAITVLHGSARFKDNRNLIVQLNDGGERVVA
                     FDRCLIATGASPAVPPIPGLKDTPYWTSTEALVSETIPKRLAVIGSSVVALELAQAFA
                     RLGAKVTILARSTLFFREDPAIGEAVTAAFRMEGIEVREHTQASQVAYINGEGDGEFV
                     LTTAHGELRADKLLVATGRAPNTRKLALDATGVTLTPQGAIVIDPGMRTSVEHIYAAG
                     DCTDQPQFVYVAAAAGTRAAINMTGGDAALNLTAMPAVVFTDPQVATVGYSEAEAHHD
                     GIKTDSRTLTLDNVPRALANFDTRGFIKLVVEEGSGRLIGVQAVAPEAGELIQTAALA
                     IRNRMTVQELADQLFPYLTMVEGLKLAAQTFNKDVKQLSCCAG"
     gene            1154..>1342
                     /gene="merD"
                     /locus_tag="OHO85_005066"
     CDS             1154..>1342
                     /gene="merD"
                     /locus_tag="OHO85_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric resistance transcriptional repressor
                     protein MerD"
                     /protein_id="HCQ2674266.1"
                     /translation="MSAYTVSQLAHNAGVSVHIVRDYLVRGLLRPVACTTGGYGVFDD
                     AALQRLCFVRAAFEAGIGL"
BASE COUNT          259 a          429 c          443 g          211 t
ORIGIN      
        1 aactgcgcca cgccaagtac gaaggcatct tggagggcaa tccggcgatc actgtgctgc
       61 acggctccgc ccgctttaag gacaatcgca acctgatcgt gcaactcaac gacggcggcg
      121 agcgcgtggt ggcattcgac cgctgcctga tcgccaccgg cgcgagcccg gccgtgccgc
      181 cgattcccgg cctgaaagac actccgtact ggacttccac tgaagcgctg gtcagcgaga
      241 cgattcctaa gcgcctggcc gtgattggct catcagtggt ggcgctggag ctggcgcagg
      301 cgttcgcccg actcggagcg aaggtgacga tcctggctcg cagcacgctg ttcttccgcg
      361 aagacccagc tataggcgaa gccgtcacgg ccgcattccg catggagggc atcgaggtga
      421 gggaacacac ccaggccagc caggtcgcgt atatcaatgg tgaaggggac ggcgaattcg
      481 tgctcaccac ggcgcacggc gaactgcgcg ccgacaagct gctggtcgcc accggccgcg
      541 cgcccaacac acgcaagctg gcactggatg cgacgggcgt cacgctcacc ccgcaaggcg
      601 ctatcgtcat cgaccccggc atgcgtacaa gcgtggaaca catctacgcc gcaggcgact
      661 gcaccgacca gccgcagttc gtctatgtgg cggcagcggc cggcactcgc gccgcgatca
      721 acatgaccgg cggtgacgcg gccctgaacc tgaccgcgat gccggccgtg gtgttcaccg
      781 acccgcaagt ggcgaccgta ggctacagcg aggcggaagc gcaccatgac ggcatcaaaa
      841 ctgatagtcg cacgctaacg ctggacaacg tgccgcgcgc gctcgccaac ttcgacacgc
      901 gcggcttcat caaactggtg gttgaagaag gcagcggacg actgatcggc gtgcaggcag
      961 tggccccgga agcgggcgaa ctgatccaga cggccgcact ggcgattcgc aaccggatga
     1021 cggtgcagga actggccgac cagttgttcc cctacctgac gatggtcgaa gggttgaagc
     1081 tcgcggcgca gaccttcaac aaggatgtga agcagctttc ctgctgcgcc gggtgaggac
     1141 aaggaggtgt gcgatgagcg cctacacggt atcgcaactg gcccataacg ctggggtgag
     1201 cgtacatatc gtgcgcgact acctggtgcg cggcttgtta cggccggtgg cctgcaccac
     1261 gggcggctac ggcgtgttcg acgatgcggc cttgcaacgg ctgtgcttcg tgcgcgcggc
     1321 cttcgaggcg ggtatcggcc tg
//
LOCUS       DAJOWB010000344         1340 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJOWB010000344 DAJOWB010000000
VERSION     DAJOWB010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.353"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            252..674
                     /locus_tag="OHO85_005067"
                     /pseudo
     CDS             252..674
                     /locus_tag="OHO85_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(685..>1340)
                     /locus_tag="OHO85_005068"
     CDS             complement(685..>1340)
                     /locus_tag="OHO85_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4-like element ISEc13 family transposase"
                     /protein_id="HCQ2674267.1"
                     /translation="SHTLNVMQKGGRAARQARMFISYSEVSIKNPDNSGQALPLTYVC
                     CREQAEDGACWHLLTSEKVASAADARRIVSHYERRWLIEEYHKAWKSGGTCVESLRMQ
                     TRDNLERMVVIKAFIAVRVLGLRQGGISEETQNDSCEKILTPTEWKLLWVKLEGKPLP
                     SQMPTLKWACLKLAKLGRWHDSKRTGRPGWVVMWDGWFRLQDMVEGYLVMKSLDQEI"
BASE COUNT          344 a          365 c          258 g          373 t
ORIGIN      
        1 tacataacac aaatatcact caggtacact actcaaacca cgctgggatt tttcctcaag
       61 ttataatcat caccccgaaa atcattcggc atttactcat taaatagtca ccccataggc
      121 ctgtacatgt tcactcagaa atatacatcc ttttctctgt cataaaccct ctgattaatc
      181 ataaataaat acttgtggca ccaatctttt tccttaacgg aacgaattgt tgtgtagaag
      241 gagataatat tatgtcaaaa aaatatcagc cattgcttat aactcattat atgtcaacat
      301 gggtcactat aacggaagca gttgagatca ctaccaaagc cataaaacaa aaaattactc
      361 ctagcgatat ttatcgtcat gccttgagtg gcaacatcct actatcggtc tatttccagt
      421 ctcctgtgat acttaagaag atacaaactt ttaatggaaa aataaaattc aggcagtttg
      481 agggagacct acttgataaa ctatgcatgc ttgacaggga cggttttatt tatggtcaaa
      541 atttaagact ctgtacagaa gccagatatg tttgtcctgt gcaacaaatt atagataccc
      601 ccctgatagg ctatgaatat gttttgattc agcgcatact ggcacgtgaa cttaaattcc
      661 cctccccaat tgtctgtctc ttgatcagat ctcctgatca agagacttca tcaccaggta
      721 accctcaacc atatcctgaa gtctgaacca gccatcccac atgactaccc aaccggggcg
      781 acctgtgcgt ttgctgtcat gccatcgccc cagtttcgcc agtttcagac aggcccattt
      841 cagtgtcggc atctgtgacg gaagcggttt tccttccagc ttaacccaca gcagtttcca
      901 ctctgtcggc gtcagtattt tctcacagct gtcattttgt gtttcttcac tgataccgcc
      961 ctgccgcagg cccagcaccc gcaccgcgat aaacgccttg ataaccacca tgcgctcgag
     1021 gttatcccgg gtctgcattc gcagcgattc cacacatgta ccaccacttt tccacgcctt
     1081 gtggtattcc tctatcagcc agcgtcgctc gtaatggctg acgatacgtc gcgcatcggc
     1141 ggcactcgcc actttttctg acgtcagcag atgccagcag gcgccgtcct ctgcctgctc
     1201 ccggcaacag acatacgtga gcgggagcgc ctggccgctg ttgtcgggat tttttatgct
     1261 gacttcgctg taactgatga acatccgggc ctggcgggct gcccgcccgc ctttttgcat
     1321 cacattcagc gtgtggcttc
//
LOCUS       DAJOWB010000345         1339 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJOWB010000345 DAJOWB010000000
VERSION     DAJOWB010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.354"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            105..>1339
                     /locus_tag="OHO85_005069"
     CDS             105..>1339
                     /locus_tag="OHO85_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCQ2674268.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLI"
BASE COUNT          381 a          264 c          317 g          377 t
ORIGIN      
        1 cattaaatca acaaaaaatt ttacctcata ttgatttgac tgctattttt gctattaata
       61 gccagaattg cagtgctttt atatctgtgt atagtgaggt tactatgtca ttcggtcagc
      121 cttgtgatga gtttcccctg agttcgctgc ctccactcat tcgtgatgcc gttattgaag
      181 ctcagcaaat tacgcaggct ccccttgggc ttgtggcagc gtctgccctg ggcgctgttt
      241 cacttgtctg ccagaatctg attgatgtct gcagactgaa tacactgcgg gggccggtct
      301 ctcttttttt cctgacgctt gccgagtccg gagaacgaaa aaccgcagtg gacaaattat
      361 taatgaagcc cctgtaccag caggagatgc agttatattc cagatacaaa agtgaactgg
      421 ctgtctggaa aaacaaggaa gagcttttaa aggctcagaa aaaagcattg ttgtcaaaac
      481 tgaataaaga actccgcaaa ggcgcggatg agtcagaaac cctcagacaa ctggaagtac
      541 ttcagaaaaa cagtgcggaa gaacctgtga ggtataaatt catttttaat gatgccacga
      601 ctgcggcaat taagaatcag ctttgtggta aatggcgttc agttggtatt atgtcagatg
      661 aagccggtat tatatttgat ggatataccc tgtctgagtt gccttttatt aacaaaatgt
      721 gggatggctc tgtattgtct gtggacagaa agaatgaacc cgaacagatg attgaaaatg
      781 ccagaatgac gttatccctc atggtgcaac ccggtctttt tgaccgatat atggagcgaa
      841 aaggttctgt tgccagggac agtggttttc ttgcccgttg tcttatcagt aaacctgcaa
      901 ccacgcaggg gaaacgcttt attaacggag ctgtgattcc gggaggatca ttaacggcat
      961 ttcatgaacg attaatggaa ctggccagag gcagtattga aaaatccagt gaggatgaac
     1021 ggtattgtct gcatttttcg cctgaagcgc aaaaaatatt tattgagcac tataatgttc
     1081 tggagcagga tttatcccct tccggaccct taagcccgtt tcggggacat gtttcaaaaa
     1141 aaacagaaaa cattgcaaga attgcagcgt tatttcagta tttcagctat ggtgaaggga
     1201 aaatctctgc cgatatcatg acgtctgctg tcgtaataag ttcgtggtac acagatgaat
     1261 ataaaaaatt atttgcgtta cctgatgaat ctgaactgca gcagaaggac gccgaggagc
     1321 tgttcgactg gctcattga
//
LOCUS       DAJOWB010000346         1328 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJOWB010000346 DAJOWB010000000
VERSION     DAJOWB010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.355"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            115..684
                     /locus_tag="OHO85_005070"
     CDS             115..684
                     /locus_tag="OHO85_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCQ2674269.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(688..915)
                     /gene="pptA"
                     /locus_tag="OHO85_005071"
     CDS             complement(688..915)
                     /gene="pptA"
                     /locus_tag="OHO85_005071"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HCQ2674270.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(966..1328)
                     /locus_tag="OHO85_005072"
                     /pseudo
     CDS             complement(966..1328)
                     /locus_tag="OHO85_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          324 a          294 c          313 g          397 t
ORIGIN      
        1 tattttgtgc gatcgcggac atgcatcacg tttgcaaatc gccaatcccg ttaatttcgt
       61 tacttattgc gttacggact acgcttaaaa aaaccttctt cgcgaggcga taccatgagc
      121 cgattcatcc ccatagaatt acaccatgcc agccgtctgt taaatcatgg cccaactgtc
      181 atgatcacca gtttcgatga gcaatcccag cggcgtaaca ttatggcagc agcctggtca
      241 atgccggtgg agtttgaacc gccacgcgtg gcgattgtgg tagataaatc gacatggacc
      301 agggagttga ttgaacgtaa cggtaaattt ggcatcgtta tcccgggcgt tgcagcaact
      361 aactggacgt gggcagtggg aagcgtgtcg ggtcgtgaag aagataaatt taattgctat
      421 ggcattccgg tggtgaaagg tccggtgctt ggtttgccac ttgttgaaga gaaatgcctg
      481 gcgtggatgg agtgccgcct gctacctgcg acttctgcgc aacaaaaata cgacacgctg
      541 tttggcgagg tggtatcagc agcggcagac gcacgggtat ttgtcgaagg ccgctggcag
      601 tttgatgatg ataagctcaa tacgttgcat catttaggtg ctggcacgtt tgttaccagc
      661 ggcaagcgag ttacggcggg ttaagcatta catgctgtaa ccaggtttct ttatcaaagc
      721 ctccatttgg ggcgcgattt cggcatccca gatagcttgc caagattctg gttgaatctg
      781 ctgtagagca atgcttatcg aactgtcttt actgttcaga tggcgaataa taacgtcggt
      841 aatatctgca gcaagtgctg ctttttgttg ttcgtccagt tcacgcggaa aacatttaat
      901 gtcgatgtgc ggcatgattg tgctcctttt gaaagatgtc agtaaaatta acacacattg
      961 tgaattttca gtcctttcca ttcaaacgtg aaatcaataa gttcatcagg gacatcgcaa
     1021 acaatatgaa tacggatttc ttctctgccg tgactctttt cacaaattgc gtaactgtca
     1081 tgctctggat tatttaattc tttcagcgga aatttttcct caaaggcttt atttagccac
     1141 ccctggtttc ctttcaccgc gaataaataa tcacctccct gtttttgtat cttctctgct
     1201 atatctttct ggcaacccat cgcatcagtt gtgatgattt ttcgtttaat atccagcatg
     1261 ttaagaagtt ctgggatagc tgtaatctca ttagatttat catccgtctt gatctgtccg
     1321 atgaccag
//
LOCUS       DAJOWB010000347         1319 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJOWB010000347 DAJOWB010000000
VERSION     DAJOWB010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.356"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..252)
                     /locus_tag="OHO85_005073"
     CDS             complement(<1..252)
                     /locus_tag="OHO85_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCQ2674271.1"
                     /translation="MNRVTAIISALVICIIVCLSWAVNHYRDNAITYKAQRDKNAREL
                     KLANAAITDMQMRQRDVAALDAKYTKELADAKAENDALRD"
     gene            complement(249..746)
                     /gene="rrrD"
                     /locus_tag="OHO85_005074"
     CDS             complement(249..746)
                     /gene="rrrD"
                     /locus_tag="OHO85_005074"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HCQ2674272.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGSSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(746..961)
                     /gene="essD"
                     /locus_tag="OHO85_005075"
     CDS             complement(746..961)
                     /gene="essD"
                     /locus_tag="OHO85_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HCQ2674273.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT          332 a          340 c          292 g          355 t
ORIGIN      
        1 atcacgcaga gcatcatttt cagctttcgc atcagctaac tccttcgtgt attttgcatc
       61 gagcgcagca acatcacgct gacgcatctg catgtcagta attgccgcgt tcgccagctt
      121 cagttctctg gcatttttgt cgcgctgggc tttataggta atggcgttat cacggtaatg
      181 attaacagcc catgacaggc agacgatgat gcagataacc agagcggaga taatcgcggt
      241 tactctgttc attgctgacc ccacaaacag atttcacgct caatctcacg acgagtcatg
      301 agacctttcc attgcttacc gccagcatat gtccagcgac gtagctgatc acatgcgcct
      361 ttgatatcgc cctggtttat tttgcgaaga agcgtcgatg ttctgaaatt gccagcaccc
      421 acgttgtaaa cgaatgagta aagagcgccg cgcgttgttt ccggtatatc gactttgatg
      481 tacgggttaa tttgtctggc gacagtggca aggtctttat tcaagagtgc cttgcattct
      541 gctttggtat acgttttacc gagcatgatg tcttttcctg tatgcccgtg acatacagtc
      601 catacaccaa caatatcttt gtatggtatg tagctgacac cctccagacc atcgttacca
      661 cttgagccag tgattaacac tgatgctata gcaattgctc cgccaccaat agcagcagca
      721 acggcttttc gtaatgatgg aggcattatt cacctctcgc agccttgcgc ttatcttctt
      781 taatcttgaa ataaaggttt gtcaggtacg tcagcaggcc aaataccaag ctacccagca
      841 cacctattgc tgcccactgt gagggcgtga ctttatcgag cagctgtaaa aaccagtagc
      901 cagcactgcc tgcggaggtg ccataggcga cacccgttgt taacttatcc atggatttca
      961 taacccccac ctcgcagatg cgggcgctgt gtaacggaaa caaaaaatgg ccaccagcgg
     1021 cccgtaaaaa acaccccgtc aaaagcaccg gcatccgcag atgccctttg cgtggcgtta
     1081 tttgatgcgc gccagatgtg gcgcaaagaa atgaaataag acttatcgaa aattaaggtt
     1141 aatttgatga tttaaaccac ttctgaagct tagtagtatg aacatgtccc cagaaggggg
     1201 ccaatactta ttattcttca tggactttgt cccgcggtct taatccgacg accgcgctac
     1261 ttttcaccct ctcgcaaatt gctatctaaa ggacgttgtc ccacgagtat tcctggatg
//
LOCUS       DAJOWB010000348         1297 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJOWB010000348 DAJOWB010000000
VERSION     DAJOWB010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.357"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..576
                     /locus_tag="OHO85_005076"
     CDS             <1..576
                     /locus_tag="OHO85_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCQ2674274.1"
                     /translation="SAYGQLSYRMTYNLVSMFSDLAILGLVDLNEVTPELLSMRKNHV
                     EILQRINTVLAGRTDEEKQADADRINLALGNITEEEIAARNEKQEELSSIQGDATSIA
                     QSLGLNYRVSTADLKMMYAPKFAAGEVFGLQEASGMKGVLFRAKDAIKAKFGARWLPA
                     KAKNSDFPGNWWIIETKHNVADVLAVIQQYA"
     gene            610..1050
                     /locus_tag="OHO85_005077"
     CDS             610..1050
                     /locus_tag="OHO85_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HCQ2674275.1"
                     /translation="MATLSDTIKPNKTYLEAVLRTALLGKTEDEYVDFFLSGLRGRLL
                     KNPRLYRSYGPYWPEIKKLLLERGYGNFGRLVDRDVRKIYRYDRPALTLIAATLYSQE
                     RFDNGQIYSAWHLLPVPEEVDDQDYEFESYDLEVEALAQAGEKT"
     gene            1047..1295
                     /locus_tag="OHO85_005078"
     CDS             1047..1295
                     /locus_tag="OHO85_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="modulator protein"
                     /protein_id="HCQ2674276.1"
                     /translation="MKKRYYTVKHGTLRALQEFADKHNVEVRREGGSKALRMYRPDGK
                     WRTVVDFKTNSVPQGVRDRAFEEWEQIIIDNALLLNAD"
BASE COUNT          360 a          310 c          346 g          281 t
ORIGIN      
        1 tcagcctatg gtcaactcag ctatcgcatg acctacaacc tggtatcaat gttttccgac
       61 cttgccatcc ttgggctggt ggatcttaac gaggttacgc cggaattgct cagcatgcgc
      121 aagaatcatg tggagatatt gcagagaatt aacacggttc ttgccgggcg caccgatgaa
      181 gagaaacagg ccgacgctga tcggataaac ctagcccttg gcaacatcac ggaggaagag
      241 attgccgcca gaaacgagaa acaagaagag ttatcatcaa tacagggtga tgccaccagc
      301 atagctcagt ctcttggtct gaattatcgc gtatccaccg ccgacctgaa gatgatgtac
      361 gcaccaaaat tcgccgctgg cgaggtattt gggcttcagg aagcctcagg catgaaaggc
      421 gttcttttcc gtgcgaaaga cgcaatcaag gcgaaattcg gcgctcgctg gctgccagcg
      481 aaggcgaaga acagcgattt cccgggtaac tggtggatta tcgagacaaa acacaacgtg
      541 gcggacgttc tggccgtcat ccaacaatac gcataacagg agcgcccggt tcgccgggcg
      601 tcgcataata tggccacact atctgataca ataaaaccga ataaaacata tcttgaggcg
      661 gtactccgta cagcgttgtt aggaaagaca gaagacgaat acgttgattt cttcctgtca
      721 gggctacgcg ggcgattact gaaaaatccc cgcctgtacc gcagctatgg tccatactgg
      781 ccggaaatta aaaaattatt actggagcgc ggttatggta atttcggtcg tctcgttgac
      841 cgtgacgttc gcaaaattta ccgttatgac cgcccggcgc taacactcat agccgcgacg
      901 ctctacagcc aggagcgttt tgataatggt cagatatact cagcctggca tttactgcca
      961 gtgcctgaag aagttgacga ccaggactat gagtttgagt cttacgattt ggaagttgaa
     1021 gccttggcac aggctggaga gaaaacttga aaaagcgata ctacacagta aagcatggga
     1081 cgctacgagc attacaagag tttgctgaca agcataacgt tgaggtgcgc agggaagggg
     1141 gaagtaaagc tctgcgcatg taccgtccgg acggaaaatg gcggacggtc gtcgatttca
     1201 aaactaacag cgttccccag ggcgtccgcg accgggcatt cgaggaatgg gagcagatca
     1261 tcatagataa cgcattgctc ctgaatgctg attaaga
//
LOCUS       DAJOWB010000349         1284 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJOWB010000349 DAJOWB010000000
VERSION     DAJOWB010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.358"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>1284
                     /locus_tag="OHO85_005079"
     CDS             <1..>1284
                     /locus_tag="OHO85_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCQ2674277.1"
                     /translation="NRFQSEFWDAVQENSWVSVSAPTSVGKSFILESWVEQFVKRNKN
                     CLVIYIVPTRALISEVYSELQKRLDPNLTNIVNIQTLPLSNVYQSEKSNVFIFTQERL
                     NLFYNHFSQIPKIDVLVVDEAHKIGDGGRGVILQHVIELTCLNNPDSKVIFASPFTLN
                     PEILLSDAPILKNKRTIKSDYVTVNQNLIWVEQKPRKTKDWLMYYFANGEKRELGNFS
                     LENAPSPESKRLPFVALCLGKGASGNVVYVNGAAEAESTSKLISASIENETEDEEILA
                     LVELSETIIHKNFALNRTLKKRVAFHYGNMPLIIKGEIERLFSKGKIDFLICTSTLVE
                     GVNMACKNIFIRGPKKGNSTPMREEDFWNLAGRAGRWGKEFQGNVICIDSDNEKIWNG
                     EPPKTKKNIFITRATDAISHDMDKLVSYIFSEHHF"
BASE COUNT          441 a          200 c          251 g          392 t
ORIGIN      
        1 ctaataggtt tcaatcagag ttttgggatg cggtacaaga aaactcatgg gttagtgtct
       61 ctgctcctac ctctgtagga aaatcattta tcctggaatc ttgggtggag cagtttgtaa
      121 aaagaaataa gaattgctta gttatttata tcgtaccaac aagggcattg atttcagaag
      181 tatattctga gttgcaaaaa cgtttggatc ctaatttaac aaatatagtt aatattcaga
      241 cactaccatt gagtaatgta taccagagcg aaaaatctaa tgtcttcata tttacacagg
      301 aaagattaaa tttattctac aatcatttct cacaaattcc aaagatagac gtcttagttg
      361 ttgatgaagc acacaagatc ggtgatggtg gaagaggtgt tattcttcaa catgtaattg
      421 aactgacttg tctaaataac cctgattcaa aagttatctt tgcaagtcct ttcaccttaa
      481 atcctgaaat attgctcagt gatgctccaa tattaaaaaa caaaagaact ataaaaagtg
      541 actatgttac agttaatcaa aatttaatat gggtagaaca aaaaccacgc aagaccaaag
      601 attggttgat gtattatttt gcaaacggtg aaaaacgcga gttaggaaac ttttctttag
      661 aaaatgcacc atctcctgaa agtaaaagat tgccttttgt agctctttgt ttaggtaagg
      721 gggcttctgg taacgtagtt tacgtaaatg gagccgctga agcagaatct acttccaagc
      781 taatatctgc aagtatagaa aatgagactg aggatgaaga aattttagct ctcgttgagc
      841 ttagtgagac aattatacat aaaaatttcg cattaaatcg cactctaaaa aagagagtcg
      901 cttttcatta tggcaacatg cctttaataa taaagggcga gattgaacga ttgtttagta
      961 aaggtaagat tgatttttta atttgtacct caactttagt tgagggcgta aacatggctt
     1021 gtaaaaatat ttttataaga ggccctaaaa agggtaattc aaccccgatg cgagaagagg
     1081 atttttggaa tcttgctggc agagctggtc gttggggaaa agaattccaa ggtaatgtca
     1141 tctgcatcga tagcgacaat gaaaaaattt ggaatggaga gcctccaaaa accaaaaaaa
     1201 atatatttat aactcgcgcc acggatgcta tttcccatga catggataag ttggtttcct
     1261 atattttttc agagcatcat tttg
//
LOCUS       DAJOWB010000350         1280 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJOWB010000350 DAJOWB010000000
VERSION     DAJOWB010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.359"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..260
                     /locus_tag="OHO85_005080"
     CDS             <1..260
                     /locus_tag="OHO85_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000235789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674278.1"
                     /translation="CESNDKNLTIYYVLPDEQYRPDLLAYRMWGIAELRWVVTLAAGL
                     EDESQGMTVGKKLKLPPATWIREMIRHFQYDGQVIGTLSIA"
     gene            270..>1280
                     /locus_tag="OHO85_005081"
     CDS             270..>1280
                     /locus_tag="OHO85_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2674279.1"
                     /translation="MPTEYARDNLGRYQTDGLSAKDFNKVFDLIRKQQRQNRRNARRT
                     LTPRIMGMRNRELEAFLSLGKKKDGTYFTPEDIRSFNTSRQAHKTKFKSTVPGITYAQ
                     LVAQSTSIDIKRANNKVSDGTGIKAATFLGLKHNLALISVNASDESVHQHHRVRIRFE
                     EWDKAVEEIAEDGAKKARIAADLCKGRVSFDCDCGRHQYWYRYMATAGNYAVAPPKEY
                     AFPKIRNPDLTGVACKHVLHAMTRFQSPTWHKAIIIALEKAAEQVAFGDDKRKTTTYF
                     KGELAKSLARNRTTTTDQAKAAREYELYLKSQDALGKKLRAKDSATDNVRRLLKKART
                     TAN"
BASE COUNT          365 a          321 c          316 g          278 t
ORIGIN      
        1 tgtgtgaatc gaacgacaag aatctgacga tttattacgt tcttcccgat gagcaatatc
       61 gcccggattt gctggcttac cgtatgtggg gcatagcaga gctacgctgg gttgtgacgc
      121 tcgccgccgg gcttgaggat gagtctcagg gtatgactgt tggcaaaaaa ttaaaactcc
      181 cacctgccac ctggatccgc gaaatgattc gccatttcca atacgacggc caggtaatag
      241 ggacattatc cattgcgtaa gggaattgaa tgccaactga atatgctcgc gacaaccttg
      301 gtcgctatca gaccgatgga ttaagtgcaa aagactttaa caaggtcttc gatcttatcc
      361 gtaaacagca gcgtcagaat cggcgaaacg cgcggcgtac actcacccca aggattatgg
      421 ggatgcgtaa ccgcgaactt gaggcattcc tcagccttgg gaaaaagaaa gatggcacct
      481 actttacgcc cgaagatata cgcagtttca acacctcaag gcaggctcat aaaaccaaat
      541 tcaagagcac ggtacccggc attacctatg ctcagctggt ggcgcagtcc accagcattg
      601 atataaaacg cgctaacaac aaagtttctg atggcacagg gatcaaagcc gcgacattcc
      661 tcgggctaaa acacaacctt gcattgatat ctgttaatgc ctcggatgag tcggtccacc
      721 agcatcaccg tgtcagaatt cgatttgagg aatgggataa agccgttgag gaaattgctg
      781 aagacggtgc gaaaaaagcc cgaatcgctg ccgatctctg caagggccgg gtatctttcg
      841 actgtgattg tggacgccat caatactggt atcgttatat ggccacggct ggtaactatg
      901 ctgtcgcgcc gccaaaagag tatgcattcc ccaaaatccg caaccctgat ctgactggtg
      961 tggcttgcaa acatgttttg cacgctatga cacgttttca gtctcccaca tggcacaagg
     1021 ccatcattat tgccctggaa aaagcagctg aacaggtagc cttcggcgat gacaagcgga
     1081 agacaacaac ctatttcaaa ggtgaactgg ctaaatcgct cgcgcgcaac cggacaacaa
     1141 cgacggatca ggctaaagct gcgcgtgagt atgagctgta tctgaaatct caggatgcat
     1201 taggcaaaaa actacgcgca aaagatagcg ccacggacaa cgttcgccgg ttgttaaaaa
     1261 aagctcgcac cacggcaaac
//
LOCUS       DAJOWB010000351         1256 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJOWB010000351 DAJOWB010000000
VERSION     DAJOWB010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.360"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            253..474
                     /locus_tag="OHO85_005082"
     CDS             253..474
                     /locus_tag="OHO85_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCQ2674280.1"
                     /translation="MVNANPCARQEFIWRFYSCKKHHYHFVIAATEDEARSQLPDGPC
                     IFTARFSTNSRNSLSYWNLPFSADVQGGL"
     gene            471..665
                     /locus_tag="OHO85_005083"
     CDS             471..665
                     /locus_tag="OHO85_005083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674281.1"
                     /translation="MKKPLVTRNEIAEAIALHTTCMPTREIPGAIANYFMITRRFYTR
                     TDKAVINKLLIAEIRDYLIE"
     gene            720..>1256
                     /locus_tag="OHO85_005084"
     CDS             720..>1256
                     /locus_tag="OHO85_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HCQ2674282.1"
                     /translation="MTGKNLNIEKPAPVASATPAPAVNVIPNIGDTIDSQTLLKMVNE
                     ARKLCSEKPVRNNDFIARVKDELEGETYEIFVGQKNGAEIDIITMTYKQALRVAARES
                     KAVRRSLIDKLEELQQANSPTPSIPQTLPEALRLAAELAEQKMQLEQQLVAAAPKVDF
                     ADRVSVANGILIGNFAKVV"
BASE COUNT          356 a          321 c          294 g          285 t
ORIGIN      
        1 atagtttcgc cgtcgtagca aattctgcga ccgggtttag cagcctgaat gattgtgcgg
       61 acagccgcag atatccgata ttgcggtatt tttgtgtccg taaaaccgcg ttacgcccaa
      121 attatggtgg ggcgtgatgg ggaggcttcg gcctgctggt ttcacaatcg ccagtctgct
      181 aaccccgtca cgtcctgcca cctgtttagc agcgggtagc aggttgttaa acctgattgt
      241 gaggccgtaa ctatggttaa tgccaatcct tgcgcacgcc aagaattcat ctggcgtttc
      301 tattcctgca aaaaacacca ctatcacttc gtcatcgcag caacagaaga cgaagcacgc
      361 tctcaattgc ctgatggccc ctgcattttt actgcccgtt tttcaactaa ctcgcgcaat
      421 tcacttagtt actggaacct ccccttctct gccgacgttc aggggggttt atgaaaaaac
      481 ctctcgtcac ccgtaatgaa atagccgaag cgatcgccct gcatacaacc tgtatgccga
      541 cacgggagat ccccggcgca attgccaact atttcatgat aaccaggcgt ttttataccc
      601 gaacagataa ggctgtgatc aacaagctac tgatagccga gatcagggat tatttgattg
      661 aataaggacg tctacgttac gcaacagtgg cagcagaaat aagaaaggag gcacatagaa
      721 tgaccggtaa aaatttgaat attgaaaaac cagcacctgt tgcttcagct acgccagcac
      781 cagccgtgaa tgtcatcccc aacatcggag acacaatcga cagccaaaca ttgttgaaga
      841 tggtcaatga ggcacggaaa ttatgtagcg aaaaaccagt tcgcaacaat gatttcattg
      901 ccagagttaa ggatgagctt gaaggagaaa cctacgaaat tttcgtaggt caaaaaaacg
      961 gcgcagaaat agatattata accatgacct acaaacaagc cctgcgagtt gccgcgcgcg
     1021 agtcaaaagc ggtccgccgt tcgctgatcg acaaactgga agaattacag caggcaaact
     1081 cccctacccc atcgatcccc cagacattac cagaagccct acgcctggct gccgagttag
     1141 cagaacagaa aatgcagttg gaacaacagc tggtggcagc agcccctaaa gtcgattttg
     1201 ccgaccgggt atcagtggcc aatggaatcc tgatcgggaa ctttgcaaag gtcgtt
//
LOCUS       DAJOWB010000352         1251 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJOWB010000352 DAJOWB010000000
VERSION     DAJOWB010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.361"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..420)
                     /locus_tag="OHO85_005085"
     CDS             complement(<1..420)
                     /locus_tag="OHO85_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HCQ2674283.1"
                     /translation="MENNDKFLSQELLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTFRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIISTKQHF"
     gene            678..1178
                     /locus_tag="OHO85_005086"
     CDS             678..1178
                     /locus_tag="OHO85_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HCQ2674284.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLNPNTEPVNILLSNNFIRINKNITLSDLTHFLISNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTISYHKIRALNKLNIKGTVELFIALCEWDKHYFKLQ
                     SCVRES"
     gene            1180..>1251
                     /locus_tag="OHO85_005087"
     CDS             1180..>1251
                     /locus_tag="OHO85_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCQ2674285.1"
                     /translation="MSLVGYNSNIIITIALISLLSACT"
BASE COUNT          425 a          230 c          180 g          416 t
ORIGIN      
        1 gaaatgttgt tttgtagaaa taatgttatt ttcaataatg cctggcaatg aatactgcca
       61 aacttcattt ttttctttta cagcaaattt gagacctaaa acttctattt gctgattcaa
      121 tgttaacgtt ctatcttcag gttcttgtgc tgttaattct ctgaatgtta ctaccagatc
      181 agggtcggta gtaattatta tctcaaaact acctgtaagc tcgccataag gaacgactat
      241 tgtgttttct ggtaactcag aaaaagcatc acttcttcct aacgaaacat cattaccaat
      301 gataacaagt agacgcccat taagtatttc atactcgcat ccattcaatg gtccgctcaa
      361 caaacgaatg gcataagatt ccaataattc ttgtgataag aatttatcat tattttccat
      421 gaataccacc cgctaattcc ataagctcta gtttagagta gtgtaacaag acggaaatat
      481 catgaccaaa ttatgatgac acagaataaa aaaattataa tttcgagaac tattttaaat
      541 gcctctatgc ataactttat ttacaattta aggtataaac cctattccct caatgttaag
      601 caaatataac aattactttt attttaactt ttacacgtaa tatttaccct aaatgatttt
      661 agagtacagg ttggcatatg caagtctttt ccagtgatgt ctacttcact gtaggcacta
      721 atgcattatt agcatcccaa aaagaatatt acagcgattt agttgcgttg gttgatcttg
      781 ggcattcgtt cgttgttatt gatgagcatc aacatcgaaa tctcaatcca aatacagaac
      841 ctgtaaacat ccttttaagc aacaatttca tcaggatcaa taagaatatt acattatctg
      901 atttaacgca tttcctgatt tcgaatcttc atacacaaaa tgtgtattcc acacaagaac
      961 cacttaccca tgatgagata gatatcctgc ggttatgtgt ttcttactcg ttaaaacaaa
     1021 tagccattat taaaggaatt gattataaaa caatatcata tcacaaaata agagccctaa
     1081 ataaacttaa cataaaaggt actgttgagt tgtttatagc actctgtgaa tgggataaac
     1141 attattttaa actccaatct tgcgtaagag aaagttgata tgtcattagt aggatataat
     1201 agtaacatca taataaccat cgcgctgata tcgttgctct ctgcatgcac c
//
LOCUS       DAJOWB010000353         1245 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJOWB010000353 DAJOWB010000000
VERSION     DAJOWB010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.362"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>1245
                     /locus_tag="OHO85_005088"
     CDS             <1..>1245
                     /locus_tag="OHO85_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674286.1"
                     /translation="EIGRARNVNGLPVLPAIIPLSDGQTISILFHSPTAENRITNSDT
                     LIAFQFLLNKKDVTHTVAPMSGRDMTLAQVTMKLANLAEKNSAKFQRAQKKKKALVDE
                     ITQLQADSDQKEDAMSDLADQVAAVEGQKVDLEQKINAVASEADSLYEENERLQTEID
                     QLNRTGGRDTIDPAGMTGGHSRALTDRLASIKNRMHMNGEVTLSNGASMKQFIEDGEG
                     YIQLTDSDGSVYMIKAKSIQGVDMADAIGKLFKAYKAGNVSEYLVQPEEHKPENVEPE
                     PAEDTGSSSPEPEVSVGAYRYALQMRPAAPGAIPEGNKAILPRPDEGDPYYEYARYGI
                     ATYDTPLSDQQMSEYDLKLLPREDSFDFLAKTLTNGPFGKYAQKALDLATSSPDEFRV
                     MLKTQFQKTFPNIAFPGGAGTE"
BASE COUNT          350 a          296 c          331 g          268 t
ORIGIN      
        1 ctgaaattgg ccgggcacgt aacgttaacg ggctgccggt cctgccagcc attattccgc
       61 tcagtgatgg tcagactatc agcattctgt ttcatagccc gacagcggaa aaccggatca
      121 ccaatagcga tacgctgatt gctttccagt tcttactgaa taaaaaagac gttactcata
      181 ccgttgctcc gatgagtgga cgtgatatga cgctggcgca ggtcaccatg aaacttgcca
      241 accttgcaga gaaaaactcg gcaaaattcc agcgtgcgca gaagaagaaa aaagccctgg
      301 ttgatgaaat aacccaacta caggctgaca gtgaccagaa agaggatgcc atgagcgacc
      361 tcgcggatca ggtggcagcg gtagaagggc agaaggtaga tctggagcag aaaattaacg
      421 ctgttgcatc ggaagcggat tctctttatg aagagaatga gcgtttgcag acggagattg
      481 atcagctcaa tcgcacaggt gggcgcgata ccattgatcc agcggggatg actggtgggc
      541 actcgcgcgc gctgacggat cgccttgcca gtatcaaaaa tcgtatgcat atgaacgggg
      601 aagtgacgct cagtaatgga gcatcaatga agcaattcat tgaggacggc gaagggtata
      661 tccagttaac cgattcggat ggcagcgtgt acatgatcaa ggctaaatcc atacagggtg
      721 tggacatggc agatgcgatc ggcaagctgt ttaaagccta taaagcgggt aatgtatcgg
      781 aatatctggt ccaaccagaa gaacataaac cggaaaacgt cgaacctgaa ccagcggagg
      841 ataccggtag ctcttcgcct gaaccagaag tctctgtagg tgcatatcga tatgccttgc
      901 aaatgcgtcc ggcggcccct ggcgcaatac ctgaaggtaa caaagcaatt ctgccgcgcc
      961 ctgatgaagg tgacccgtat tatgaatatg cacgctacgg cattgctact tacgataccc
     1021 cgctttctga tcagcaaatg agtgagtacg acctgaagtt attgcctcgc gaggattctt
     1081 tcgacttcct ggcgaagaca cttactaatg gtccgtttgg caaatatgca caaaaagctc
     1141 tggatctggc caccagctca ccagacgagt tccgcgtaat gctgaaaact cagtttcaaa
     1201 aaactttccc caatattgcg tttccggggg gcgctggcac cgaga
//
LOCUS       DAJOWB010000354         1240 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJOWB010000354 DAJOWB010000000
VERSION     DAJOWB010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.363"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..524)
                     /locus_tag="OHO85_005089"
     CDS             complement(<1..524)
                     /locus_tag="OHO85_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCQ2674287.1"
                     /translation="MAVRISGVLKDGAGKPIQNCTIQLKARRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFEQMVEEVARNASVVAQNTAAAKKSASDAGTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGT"
     gene            complement(589..1188)
                     /locus_tag="OHO85_005090"
     CDS             complement(589..1188)
                     /locus_tag="OHO85_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HCQ2674288.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMVG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT          265 a          388 c          313 g          274 t
ORIGIN      
        1 gttcctgcgc tggaagacgc tgactgagcc gacgacgcgg cctgtccggc tgacgtgctg
       61 gctgcacgtg ctgaatctgc agcatcagtc gcatgggttg ccgcctcacg ggcagatgtg
      121 ccggcatcac tggctgactt cttcgcggct gccgtgttct gtgccaccac ggacgcgtta
      181 cgcgccacct cttccaccat ctgttcaaaa cggcgcagtg cctccggacg ggcatcatcc
      241 tccgtcatgg caccgagaaa atcattcagc gtaccgggtt gagaatcttc atacacggtg
      301 atggtcccgg catgtgacgg cgggaatcct tccaccaaca gaataacgct gtactgcccg
      361 tactcaacgt ccatgctgta acgcccggct tcatccgggt tttctgaggc cagcgtgttc
      421 accaccaccg tggtgctgtt gcgcctggcc tttagctgaa tggtgcagtt ttgtatcggc
      481 ttacctgcgc catctttcag tacacctgaa atccgtactg ccatattccc cccacaaaaa
      541 agcccgcctg aaccggcggg ctgtcataac actgtgttac ctggctaatc agaacttata
      601 accgacaccc acgatgaaac cgtcagtgcg ccagtcgcca ctgccggagc cttcataagc
      661 aatatcaatg gccacggatt cggtcgggtt aaactgcacg ccagctcccc acgccagaga
      721 cgtgttgctg tggcgaccgt catcacttcc ggtcagcacg tcgtgcgttt tccccttgtt
      781 gtcagttacg cggagataat ccccggagaa agtcgacaca cggctgtaag ccatacccgc
      841 catcgcatac gcgctgaacc attcattcac gcgcacagac ggccccacca tcacgctgaa
      901 ccagcggtta cgcacggaat cttcatgcca gcgggtatcg ctgtaacggg taagctggcg
      961 attcttgtct cctgcatagc tgaatgacgt caccagcccc agtgtgtccg taaactcata
     1021 acggtatttc acgttaatcc cgttcagatc atcactgccg ggaacgttcg tccgggcatg
     1081 aagatacccc gcgctcagcg tggactgatg ttcagacgcc catgcaggcg caccggatac
     1141 ggccagacaa atggctgcgg acaaaatggc ggcataaagt ttacgcataa ttacctctcg
     1201 cttttctgca ataaaaaagg cgtcatttct gacgcccgtt
//
LOCUS       DAJOWB010000355         1237 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJOWB010000355 DAJOWB010000000
VERSION     DAJOWB010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.364"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          382 a          278 c          215 g          362 t
ORIGIN      
        1 ggaataattc gaactctatt agattctatc cgtattgttt cgtccagcga ataaatagtt
       61 gcacctaata ctgctacact tttgtcagac aatgttttcc ttgaggagga agactgacaa
      121 ttttcataaa atgcaacatc tttttcaaca acagtcactt tgaacggcac taatggttac
      181 ggagtagagt ctcgtaaagt aacagccata accactcaat ataatttacc cacgataaga
      241 gaactgacgg ataagtcaac ctgattttcg agttattctt aaccgacttt tacgttttct
      301 ccctcttggg tataacgtag ctatccacat aagcgtgatt attttgaccc gtcggtacgc
      361 cgcttgcaga tattgtaaca ggttaatgaa cggcaccgag cttttgctgc tgccatccac
      421 ataattcact tttcctgtac cacagtgcgg ccccaatata cgctgcgttg gcgcagcacg
      481 tcgccagatc actcttgcat aggacatcaa cagcggattg ttgacgcatg tagccgcaac
      541 gaaaaaatct gttaaaagga gcacttacct acgcaacagg agtcaaaaaa acctctcctg
      601 cagtgccata caatataaag ctgttaaccc atcgtcccaa agataaacgg acactgcaaa
      661 tattctgggc tacagagctt aaatgctccc cgtagcatca gcatgagaat atgatttttg
      721 taatcagttt tatagtctgc tcgattagac ctcgtttgtc actgggtgtt gatgcgataa
      781 gatgcatcgc ccactccagt tgataattta tgatgcttgg cgattgctca tattcagagc
      841 gcacaatctg gaataagttt cctttaagag ctcaactatt ttacgaaact atttcgactt
      901 tgcatcgttg agaaagcacc aataatatta acagcccctt gcagtaattc cgctcataag
      961 aataccagag cgttttttca acaattacaa tgaattatta accatctgaa gaattgtagt
     1021 cacgtattca attaataaaa tcctcgtaaa ttttgcagaa ccaattcagg atatatcttc
     1081 ggcaatgata ttatgacaga aatgccattt tcaaaatcac catatcgatt caaaaagtcg
     1141 agatggttct cccaaaacca tctatatttt ccatctatat tttccaaccg cctgcactaa
     1201 gaatgctcga caaataatca acagtagata ttgttgc
//
LOCUS       DAJOWB010000356         1226 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJOWB010000356 DAJOWB010000000
VERSION     DAJOWB010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.365"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..343
                     /locus_tag="OHO85_005091"
     CDS             <1..343
                     /locus_tag="OHO85_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674289.1"
                     /translation="GAHTHSVSGTAASAGNHTHSVTGASAVSQWSQNGSVHKVVSASS
                     VNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVK
                     NIAFNYIVRLA"
     gene            343..951
                     /locus_tag="OHO85_005092"
     CDS             343..951
                     /locus_tag="OHO85_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCQ2674290.1"
                     /translation="MAFRMSEQPRTITIYNLLSGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     ASLLAAWKKYRVLLNRVDTSTAPDVDWPAAPKCNSLELITCE"
BASE COUNT          358 a          245 c          317 g          306 t
ORIGIN      
        1 cggggcacat acgcacagtg tgagcggtac agccgcaagt gccggaaacc atactcatag
       61 tgtcacaggc gcatcagcag tcagccagtg gtcacaaaat ggatcagtac ataaggtagt
      121 gtctgcgtcc agtgtgaata caagtgctgc aggagcgcac actcatagtg tcagcggcac
      181 agctgcatct gcaggtgctc acgcacatac tgtcggtatt ggtgctcata cgcactctgt
      241 tgcgattggc tcacatggac acaccatcac cgttaacgct gctggtaacg cggaaaacac
      301 cgtcaaaaac atcgcattta actatattgt gaggcttgca taatggcatt cagaatgagt
      361 gaacaaccac ggaccataac aatttataat ctgctgtccg gaactaatga atttattggt
      421 gaaggtgatg catatattcc gcctcataca ggtctgccag caaacagtac cgatattgca
      481 ccgccagata ttccggctgg tttcgtggct gttttcaaca gtgatgaggc atcgtggcat
      541 ctcgttgaag accatcgggg taaaaccgtc tatgacgtgg cttccggcga cgcgttattt
      601 atttctgaac ttggcccatt accggaaaat gtcacctggt tatccccgga aggggagttt
      661 cagaagtgga acggcacagc ctgggtgaaa gatgcagaag cagaaaaact gttccggatc
      721 cgggaggcgg aagaaacaaa aaacagcctg atgcaggtag ccagtgagca tatagcgccg
      781 cttcaggatg ctgtggatct ggagatcgca acggaagaag aggcgtcgtt gctggcagca
      841 tggaagaagt atcgggtgtt gctgaaccgt gttgatacat caactgcacc tgatgttgat
      901 tggccagcag caccgaagtg taattcatta gaactcatca cctgtgaata atatctgttt
      961 ttgagtaagt tagtaataat cacttaaatg tgttatgagt ttgttgaggc gtgatttgca
     1021 ataggataga gagtacgatt atggattgag aataagaaag cgccatgaaa gagtttcatg
     1081 gcgagtacat aaacgtacaa aatatgatga aagtagagtg gcataagcca ctatgttaac
     1141 ttatagcaat agggaagata agtaaataat atttcttgag tatagtgtaa gtcgttcatt
     1201 gtaaagcgtt aatgttatct atattg
//
LOCUS       DAJOWB010000357         1222 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJOWB010000357 DAJOWB010000000
VERSION     DAJOWB010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.366"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..1177
                     /locus_tag="OHO85_005093"
     CDS             <1..1177
                     /locus_tag="OHO85_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCQ2674291.1"
                     /translation="KYGLPEGTLYAIAATESGGDPNAKSPLTRSPDGKLSGGALGMFQ
                     FTRVAREETGLSREDSFNPEKSAEAAALLMSKYLKQANGDLNEAITAYNAGFGTINKW
                     KKGTGDLSKENREYAIKVNTHRARYLGGEIYTPRAGTQGGRPQQSGQSPRMDNLPENA
                     FVDQSTGLAFTPGENPFEKGGLVDRIGELTGVNDLASKFLNGRGMRREVVQGTLEERA
                     RGKGTATAAGNVYVDTPMPVEEARPVANNSSYFDQLGAQMGIDGLYDKLINARGMRSN
                     NSPEPASTSQVTTAANDLQQPTGRMQIDGQVISDLGGSGAKPTMQLADNTVSLDGETK
                     RLFAQMTSLLARIEEHTKDSAKGQGTVVKVSTPQPGVMRTVPLSIDDPLMNDYARVD"
BASE COUNT          340 a          277 c          335 g          270 t
ORIGIN      
        1 taaatatggt cttcctgaag gcactcttta cgcgattgct gcaactgaat caggagggga
       61 tcctaacgca aagtctccgc ttacaagatc accagatgga aagttaagtg gtggcgcact
      121 cgggatgttc cagtttacgc gcgttgctcg tgaggaaact ggattatccc gggaagattc
      181 ttttaatccg gaaaaatcgg cagaagctgc ggctcttctc atgagcaagt atctgaagca
      241 agccaatgga gacttaaacg aggccatcac tgcatataac gctgggtttg gcactattaa
      301 taagtggaaa aaaggcacag gtgacttatc gaaagaaaac cgtgagtacg cgatcaaggt
      361 caatactcat cgtgctcgct atttaggtgg tgaaatctat acacctagag caggaacaca
      421 aggaggaaga ccgcaacaaa gtggccaatc accaagaatg gataaccttc ctgaaaacgc
      481 ctttgttgat cagtctacgg gcctggcgtt tacccctggc gaaaacccgt ttgaaaaagg
      541 gggattagta gaccggattg gtgaacttac gggggtgaac gatctggcca gcaaattcct
      601 gaatggccgg gggatgcgtc gggaagttgt tcagggaacg ctcgaagaac gtgcacgagg
      661 gaaggggacc gcaacggctg ctggcaatgt gtatgttgat acacctatgc cagttgaaga
      721 ggcacgtccg gtggccaaca actcaagtta ctttgaccag ctcggcgcac aaatggggat
      781 tgatggactg tatgacaaac tcattaatgc ccggggaatg cgctcaaata attctcctga
      841 accagcctcc acgtcccagg tgacgactgc cgccaacgat ttgcagcaac caaccggtcg
      901 tatgcagata gacggacagg ttattagtga ccttggcggc tccggtgcca agccgacaat
      961 gcagttggct gataataccg tttcacttga tggtgaaacg aagcggctgt ttgcgcagat
     1021 gacctcattg cttgccagga ttgaagagca cactaaagac tcggcgaaag gccagggaac
     1081 tgtcgtaaag gtcagcacgc ctcaaccggg cgttatgcgc acggtgccac tgtcaattga
     1141 tgatccgttg atgaatgact acgcgagagt tgattgatgg ccaacaataa tgaaattgat
     1201 cctttactga cgctggagtt at
//
LOCUS       DAJOWB010000358         1209 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJOWB010000358 DAJOWB010000000
VERSION     DAJOWB010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.368"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            624..875
                     /locus_tag="OHO85_005094"
     CDS             624..875
                     /locus_tag="OHO85_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCQ2674292.1"
                     /translation="MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            872..1159
                     /locus_tag="OHO85_005095"
     CDS             872..1159
                     /locus_tag="OHO85_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCQ2674293.1"
                     /translation="MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYNLASERMR"
BASE COUNT          336 a          296 c          280 g          297 t
ORIGIN      
        1 cctgtcctgt ggacagacag atatgcacct cccaccgcaa gcggcgggcc ccgaccggag
       61 ccattttagt tacaacactc aaatacaacc gccaagaaaa acccggtcca gcgcagaacc
      121 gaaaccacaa agcccctccc tcataactga aaagcggccc cgccccggcc cttcgggccg
      181 gaacagagtc gcttttaatt atgaatgttg taactacgtt gtcatcgctg tcagtcttct
      241 ggctggacgt atcgtgtaca cgctcgtaag cggccctaac ggcccgctaa cgcggagata
      301 cgccccgact tcgggtaaac cctcgtcggg accactccga ccgcgcacat aagctctctc
      361 atggctgaca gcgggcatgg cttagcaggg cggggatggg taaggtgaaa tctatcaatc
      421 agtaccggct tacgccgggc ttcggcggtt ttactcttgt gccatatgta gctacattat
      481 accgccttcc atgccgctca cgcggcatca gaacaataaa accagcatta aatgtatttg
      541 caaaagtatg agtaatacag acttgttaac cggtcctttc agaggtatct ttaagacttg
      601 ttaaacagtc tcaaaggtac gttatgccta acatcattct gtctgacacc agtgcaagtg
      661 tcagtgaact gaaaaaaaat cctatggcta cagtcagtgc cggagatggt tatcctgtcg
      721 ccattcttaa ccggaatcag ccggcattct actgtgtgcc tgcggaactc tatgaaagga
      781 tgcttgatgc gctggatgac caggaactgg tgaaactggt tactgaacgc agcaatcagc
      841 cactgcatga tgtggacctg gacagttatt tatgacttat acagtaaaat tcagggatga
      901 tgcgctcaaa gaatggctga aactggataa atccattcaa cagcagtttg cgaaaaaact
      961 aaagaaatgc agcgaaaatc ctcatattcc ttccgcaaaa ctgcgtggat taaaagactg
     1021 ctacaaaatc aaacttcgcg catcggggtt tcgtctggtc tatcaggtta ttgatgacat
     1081 gctaattatt gcggttgtcg cggtaggtaa acgtgaacgt agtaacgttt ataatctcgc
     1141 cagtgagaga atgagatagt tatctttaaa gctgactgat attactttaa aagtatttct
     1201 gatagttat
//
LOCUS       DAJOWB010000359         1207 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJOWB010000359 DAJOWB010000000
VERSION     DAJOWB010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.369"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            59..349
                     /locus_tag="OHO85_005096"
     CDS             59..349
                     /locus_tag="OHO85_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCQ2674294.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANCSRGKNL"
     gene            373..>1207
                     /locus_tag="OHO85_005097"
     CDS             373..>1207
                     /locus_tag="OHO85_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCQ2674295.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKYLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNDLFPNLNTLLINNNLLSEIKIFYSNFCNVQTLNAANNQLKYINL
                     DF"
BASE COUNT          430 a          230 c          190 g          357 t
ORIGIN      
        1 cgccgtttaa aattcgttta gaaaatacgt ttaacgataa ttaaaaggat tctgttaaat
       61 gaaattaaaa atagttgcgg tggttgtaac tggtttgtta gctgcgaacg tagcacacgc
      121 tgccgaagtc tataacaagg atggtaataa actcgacctt tatggcaagg ttaccgctct
      181 acgttatttt actgatgata agcgtgacga tggtgataaa acttatgccc gtctcggctt
      241 taaaggagaa acgcaaatca atgatcaaat gattggtttt ggtcactggg aatatgattt
      301 taaaggctat aacgatgaag ccaactgctc gcgcggcaag aatctctgat tgagttaagt
      361 gcccatcaca atatgatcac tgaccttata ttacacaatc atcccaggat gaaaacaatc
      421 actttaaacg acaaccatat tgcacattta aacgccaaaa acactacaaa actggaatat
      481 ttaaacttaa gcaataacaa tttactgcca accaatgaca ttgatcaact aatatcatca
      541 aaatatcttt ggcatgtatt agttaacggc atcaacaatg atccacttgc acaaatgcag
      601 tactggactg cagtaagaaa tataattgat gacactaatg aagtgaccat tgatttatca
      661 ggacttaatt taaccactca accaccaggg ctgcaaaact tcacctctat caatcttgat
      721 aataaccaac tcacacattt tgatgcaacc aactacgata gactcgtaaa gctaagtctg
      781 aatagtaatg ctcttgagtc aataaatttt cctcaaggca gaaatgtaag tattacacat
      841 atatctatga ataataatgc tctcagaaat attgatatag ataggctttc atcagttact
      901 tattttagtg cggcacataa tcaactagag tttgtgcaat tagaatcttg cgaatggctg
      961 caatacctga atctcagcca taatcaatta actgatattg ttgcaggtaa taaaaatgaa
     1021 ctcttactgc tggatctatc ccataataaa ctaacaagtt tacacaatga cttatttccc
     1081 aacttgaata cgttacttat taacaacaat ttgctttctg aaattaaaat attctatagc
     1141 aacttctgca atgttcagac attaaacgct gctaacaacc agttgaaata tataaatctt
     1201 gatttcc
//
LOCUS       DAJOWB010000360         1205 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJOWB010000360 DAJOWB010000000
VERSION     DAJOWB010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.370"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            75..1163
                     /gene="ompF"
                     /locus_tag="OHO85_005098"
     CDS             75..1163
                     /gene="ompF"
                     /locus_tag="OHO85_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCQ2674296.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT          331 a          268 c          293 g          313 t
ORIGIN      
        1 cgtaaatttt tattgacaga acttattgac ggcagtggca ggtgtcataa aaaaaaccat
       61 gagggtaata aataatgatg aagcgcaata ttctggcagt gatcgtccct gctctgttag
      121 tagcaggtac tgcaaacgct gcagaaatct ataacaaaga tggcaacaaa gtagatctgt
      181 acggtaaagc tgttggtctg cattattttt ccaagggtaa cggtgaaaac agttacggtg
      241 gcaatggcga catgacctat gcccgtcttg gttttaaagg ggaaactcaa atcaattccg
      301 atctgaccgg ttatggtcag tgggaatata acttccaggg taacaactct gaaggcgctg
      361 acgctcaaac tggtaacaaa acgcgtctgg cattcgcggg tcttaaatac gctgacgttg
      421 gttctttcga ttacggccgt aactacggtg tggtttatga tgcactgggt tacaccgata
      481 tgctgccaga atttggtggt gatactgcat acagcgatga cttcttcgtt ggtcgtgttg
      541 gcggcgttgc tacctatcgt aactccaact tctttggtct ggttgatggc ctgaacttcg
      601 ctgttcagta cctgggtaaa aacgagcgtg acactgcacg ccgttctaac ggcgacggtg
      661 ttggcggttc tatcagctac gaatacgaag gctttggtat cgttggtgct tatggtgcag
      721 ctgaccgtac caacctgcaa gaagctcaac ctcttggcaa cggtaaaaaa gctgaacagt
      781 gggctactgg tctgaagtac gacgcgaaca acatctactt ggcagcgaac tacggtgaaa
      841 cccgtaacgc tacgccgatc actaataaat ttacaaacac cagcggcttc gccaacaaaa
      901 cgcaagacgt tctgttagtt gcgcaatacc agttcgattt cggtctgcgt ccgtccatcg
      961 cttacaccaa atctaaagcg aaagacgtag aaggtatcgg tgatgttgat ctggtgaact
     1021 actttgaagt gggcgcaacc tactacttca acaaaaacat gtccacctat gttgactaca
     1081 tcatcaacca gatcgattct gacaacaaac tgggcgtagg ttcagacgac accgttgctg
     1141 tgggtatcgt ttaccagttc taatagcaca cctctttgtt aaatgccgaa aaaacaggac
     1201 tttgg
//
LOCUS       DAJOWB010000361         1202 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJOWB010000361 DAJOWB010000000
VERSION     DAJOWB010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.371"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            329..>1202
                     /locus_tag="OHO85_005099"
     CDS             329..>1202
                     /locus_tag="OHO85_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCQ2674297.1"
                     /translation="MAGLKNTPYNAVHWSQLAPEEQIRFWEDYEAGRATTFLVEPERK
                     RTKRRRGEHSTKPKCENPSWYRPERYKALSGQLGHAYNRLVKKDPVTGEQSLRMHMSL
                     HPFYVQKRTYVGRKYAFRPEKQRLLDAVWPVLVSFSDAGTHTVGMSVSRLAREISPKD
                     SKGKVIPELEVTVSRLSRLLAEQVRFGVLGVSEETLWDRETRQRLPRYVWITPAGWQM
                     LGVDMVKLHEQQQKRLRESEIRQQLIREGVLREDEDISVHAARKRWYLQRSQDALKHR
                     RAKAAASKRARRLKK"
BASE COUNT          321 a          301 c          349 g          231 t
ORIGIN      
        1 attaacgtaa agcgggtata tttcggctgt tattagctgt gcagagggtg gcactctgtg
       61 gagcaaagcg gcgaaagccg gacggcagaa tgcgccataa ggcattcagg agagatggca
      121 tgtacgggca gtaagtcaga agactgaaga tgttccggaa gccataaaag gaaaaccccc
      181 actatctttc ttacgaactt ggcggaacga cgaaagatag tgggggcctc acagaatacg
      241 ggtaaagtat aatgaaaccg taccagagat tcaaccctgt gcagtgtata aatacacggc
      301 acaatcgctc cgccataagc gacagcttgt ggcaggtctg aagaatactc catataacgc
      361 agtacactgg agtcagttag cacccgaaga gcagatccgt ttctgggaag actatgaagc
      421 gggaagggcg accactttcc tggttgaacc ggaaaggaag cgcacgaagc gtcgtcgtgg
      481 tgagcactcc accaaaccca aatgcgaaaa tccgtcctgg tatcgtcctg agcgctataa
      541 ggcgctgagc gggcagctcg ggcacgccta caaccgtctg gtgaaaaagg acccggtgac
      601 cggcgagcag agcctgcgca tgcacatgtc tctgcatcct ttttacgtgc agaaacgaac
      661 gtatgtcggt cgcaaatatg ctttccgtcc ggaaaaacaa cgcctcctcg atgccgtctg
      721 gccggttctg gtcagcttca gtgatgcggg cacacatacc gtaggcatga gtgtttcccg
      781 tctggccaga gaaatcagcc cgaaagacag caaggggaag gttattccgg aactggaagt
      841 gacggtctcc cgcctttccc gtttgctggc cgaacaggta cgttttggtg tgctgggtgt
      901 ttcagaggaa accctgtggg accgtgaaac ccgccagcgt ctgccacgtt acgtctggat
      961 aacaccggca ggctggcaga tgctgggcgt cgacatggta aaacttcacg aacagcagca
     1021 gaaacgactg cgtgaaagtg aaatccgcca gcagctcatt cgggaaggtg ttctgcgtga
     1081 ggatgaagat atctccgtac atgcggccag aaaacgctgg tatctgcagc gcagtcagga
     1141 tgcgctgaaa caccgtcgtg cgaaagcagc agccagtaag cgcgccagac gcctgaagaa
     1201 ac
//
LOCUS       DAJOWB010000362         1198 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJOWB010000362 DAJOWB010000000
VERSION     DAJOWB010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.372"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>1198
                     /locus_tag="OHO85_005100"
     CDS             <1..>1198
                     /locus_tag="OHO85_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pcar"
                     /protein_id="HCQ2674298.1"
                     /translation="PLTPSVITDEKAQSYIEALNFACSRPDIRNIAVTGPYGAGKSSV
                     LLTWERAEDNDFRVMTVSLADFEMQRAYPGDSLSVEGKPDYDDNEKKAGKAEEKTIEY
                     SILQQLLYKEKKSVLPYSRLERISDVSACQIAMMTASLLFILASTATGLLFLFPDYIR
                     EKLSLSPELSQFLLELPVLARFGSAGIFLFTALFFALKKLHRTGVFDRRVSIDKVDIL
                     KGAITTRPAAPSLLNVYIDEIVYFFEQTQYNVVIFEDLDRHNDGAIFIKLREINQLIN
                     NCLPTDNPVRFIYAVRDNLFITPESRTKFFDFVIPVIPVMDSENASEHFLSKFTLDEL
                     RQEGFKDCLARLALFIPDMRVMHNIANEFRLYRNIVNNGGDLKRLISLITYKNLYAED
                     YHRIDQ"
BASE COUNT          343 a          257 c          262 g          336 t
ORIGIN      
        1 aaccactgac tccctcagtc attacagatg aaaaagcaca aagctatatc gaagctttga
       61 attttgcctg ttctcgcccg gatatcagaa atattgcagt aacagggccg tacggggccg
      121 gtaaaagttc ggtattactg acatgggaaa gggcggagga taatgacttt cgggtgatga
      181 ccgtatcact ggctgacttt gaaatgcagc gggcataccc cggagattcg ctgtcagttg
      241 agggtaagcc cgactatgat gataacgaaa aaaaagcggg aaaagccgaa gagaaaacaa
      301 tcgagtacag catcctgcaa caactgctct ataaagagaa aaaaagtgtg ttaccctatt
      361 cccgtctgga gcgcatttct gacgtaagtg cgtgtcagat agccatgatg acggccagtc
      421 tgttattcat actggcttcg acggcaactg gtctgctttt tcttttcccg gattacatcc
      481 gggaaaagct ctccctgtcg ccagaactga gtcagtttct gctggagttg cctgtactcg
      541 ctaggtttgg tagcgccggg atatttcttt ttactgcttt attctttgca ctgaagaaat
      601 tgcaccgtac aggggtattt gatcggcgtg taagtattga taaagttgac atactgaaag
      661 gtgccattac aacacgccct gcggcccctt ctcttctcaa tgtgtacatt gatgaaatcg
      721 tctacttttt cgaacagaca caatacaatg tagtcatatt tgaggatctt gaccgtcata
      781 atgacggagc catttttatc aaactccggg aaataaatca gcttatcaat aactgcctcc
      841 ccacagacaa tcctgtccga tttatctacg ctgtcaggga taatcttttc ataacaccag
      901 aatcacggac aaaatttttc gatttcgtca taccagtaat cccagtaatg gacagtgaga
      961 atgcttctga gcattttcta agtaaattta cacttgatga actcaggcag gaaggattca
     1021 aagactgcct tgccagactg gctcttttta ttcctgatat gcgggttatg cataacattg
     1081 cgaatgaatt tcgtctttac cggaatattg tgaataatgg aggagattta aagcgtctta
     1141 tttcactgat cacctataaa aacctttatg ctgaagacta tcacaggata gatcagaa
//
LOCUS       DAJOWB010000363         1188 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJOWB010000363 DAJOWB010000000
VERSION     DAJOWB010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.373"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..567)
                     /locus_tag="OHO85_005101"
     CDS             complement(<1..567)
                     /locus_tag="OHO85_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674299.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLL"
     gene            complement(654..860)
                     /locus_tag="OHO85_005102"
     CDS             complement(654..860)
                     /locus_tag="OHO85_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCQ2674300.1"
                     /translation="MLTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
BASE COUNT          284 a          293 c          338 g          273 t
ORIGIN      
        1 caggagcacg tactgatcca tcagatccgt cagtgctgcg ctctgcttct gacggttgat
       61 gatccattcc tccagctctt tttctgattt ccgtttcaga ttctccgtcc agtcgtcaag
      121 gcagtttccg gtcattaaat caagccagca aagccagacc agtttgaggc gcaactccgt
      181 gccaccaaac tgacggaaga ggctgatcag tttatttgcc agcgaacgct gcctcagacg
      241 ttcacattca gacaggagaa tgccttccag catcggatga tgaaccagca gatgttcact
      301 ggtgcataaa ttcactatca gatcatcatc caggggcggc gtggtccgtt tgtccggacc
      361 ataaatcagc cggtgcagac tctggggtac gatggcaatc tccggttttt caccattgaa
      421 tgacacccac ccgggcgtca gattatggaa gtagtagata ttcggatgac acacgcccca
      481 ggatacggcg tgccgggtga agccggtcag ccatttctgg tagctttcgt ggagcagctc
      541 gtaacgggtc tgatactgtg gtttcatttt catattcctc acgggggata acaggcagca
      601 gaacgccgcc ggcaggccgg agactgcgtc tgttgcagat gaacggttaa gaattacgga
      661 cgggactgtg caatacgcgc ctgcagccag gcttccactt cgcttttcag ccatcgggag
      721 ctgcggccca ttttgatggg cgcaggaaag tcaccatcct tgatgagttt gtaaaaccac
      781 ttatcggtca ggccggtcag ttgagtgata aatgccatgt cgaccatctg gtcatccatc
      841 agtgaaactg gggtgagcat gagaatgttc ctccggatgt tgttaaacca gccggtgtgg
      901 tcatgaaaac aacgcaccac cttcaccggc ataacaaccg gggagaaacg taaaaaataa
      961 tcgggaatgt gaccgccagg agataaacgg ggaggttacg gtggctgagc cgtaccggta
     1021 tgccgggtgg ttgttacgaa acgatgcttc cgcacgcgcg cgcgataaag aagggatgat
     1081 tttcgtgagg aagaggggaa aactggcgtt ctgataattg cagagttatt tacggggcgg
     1141 ttacgatggg gcagaccgtg tccggtgggc aggcgacagg tatcaccg
//
LOCUS       DAJOWB010000364         1181 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJOWB010000364 DAJOWB010000000
VERSION     DAJOWB010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.374"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(203..1150)
                     /locus_tag="OHO85_005103"
     CDS             complement(203..1150)
                     /locus_tag="OHO85_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease"
                     /protein_id="HCQ2674301.1"
                     /translation="MYLKILATALSAPVAFAALASDTGLSFTPEKISTEIDFGTLSGK
                     AKERVYLPEEKGRKASQLDWKYSNAPIVKGAFNWDLLPRVSVGASGWTTLAGRGGNMV
                     DRDWLDTSNPGTWTDESKHPNTRLNFANEFDLNIKGWLLNQPDYQLGLMAGYQENRYS
                     FTAKGGSYIYSSEGGFRDETGAFPDGERAIGYKQHFKMPYIGLTGNYRYDSFEFGGSF
                     KYSGWVKASDNDEHYNPEKRITYRSDVNNQNYYSVSLHAGYYITPAAKVYVEGTWNRI
                     TNKKGDTSLYSRNLNISTKNGAGIESYNFVTTAGLKYYF"
BASE COUNT          337 a          277 c          217 g          350 t
ORIGIN      
        1 tcgataacac tgtgtcgtta tgcatgaaga gtggcagggc agcatcttta aaaatacaag
       61 agttaacaga aacactttta tcgttgagca catataccct ccacatatta taaaaaatca
      121 aaagaccacc atcagaagtg ctggcctttt gtttatcata tttaaagtgt catgaaaaag
      181 ttaaaaacag gcatgcttca ggttaaaaat aatacttcag accagctgtg gtcacaaagt
      241 tatagctttc aatgcctgca ccatttttgg tggatatatt caaatttctg gagtacagag
      301 aagtatcacc tttcttatta gtaatacggt tccatgttcc ttcgacataa actttagctg
      361 caggtgtaat ataataacca gcatgtagtg agacagagta atagttctga ttatttacat
      421 cgctacgata ggtgatcctt ttctcagggt tgtaatgctc atcattatcc gatgccttca
      481 cccatccgct atatttgaaa ctccccccga actcaaagct atcataccga taattacctg
      541 ttaaccctat atagggcatt ttaaagtgtt gtttgtagcc tatagctctt tcaccatctg
      601 gaaatgctcc cgtttcatcc cggaagcctc cctcactact atagatataa gagcctcctt
      661 ttgctgtaaa gctataacga ttttcctggt aaccagccat aagacctaac tgatagtcgg
      721 gttgattcag gagccatccc ttaatattca ggtcgaattc attagcaaaa tttagacggg
      781 tattcggatg tttactctca tcagtccagg ttccgggatt gctcgtatcc agccagtccc
      841 tgtccaccat attgcctcca cgaccagcta atgttgtcca ccctgaggca ccgacagata
      901 ctctgggtaa cagatcccag ttaaatgccc ccttgacaat tggcgcatta ctgtatttcc
      961 agtccagctg gctggctttg cgcccttttt cttcaggcag ataaacccgt tctttggcct
     1021 tgccacttaa agtgccaaaa tcaatctccg tactgatttt ctccggtgta aaagaaagcc
     1081 cagtatcaga agccagtgca gcaaacgcta caggagctga gagtgcagta gcaagaatct
     1141 ttaagtacat agacaagtta actccattcc actcgtttgt t
//
LOCUS       DAJOWB010000365         1161 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJOWB010000365 DAJOWB010000000
VERSION     DAJOWB010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.375"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>1161
                     /locus_tag="OHO85_005104"
     rRNA            <1..>1161
                     /locus_tag="OHO85_005104"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          312 a          246 c          370 g          233 t
ORIGIN      
        1 tctttatggg gtgactgcgt accttttgta taatgggtca gcgacttata ttctgtagca
       61 aggttaaccg aataggggag ccgaagggaa accgagtctt aactgggcgt taagttgcag
      121 ggtatagacc cgaaacccgg tgatctagcc atgggcaggt tgaaggttgg gtaacactaa
      181 ctggaggacc gaaccgacta atgttgaaaa attagcggat gacttgtggc tgggggtgaa
      241 aggccaatca aaccgggaga tagctggttc tccccgaaag ctatttaggt agcgcctcgt
      301 gaattcatct ccgggggtag agcactgttt cggcaagggg gtcatcccga cttaccaacc
      361 cgatgcaaac tgcgaatacc ggagaatgtt atcacgggag acacacggcg ggtgctaacg
      421 tccgtcgtga agagggaaac aacccagacc gccagctaag gtcccaaagt catggttaag
      481 tgggaaacga tgtgggaagg cccagacagc caggatgttg gcttagaagc agccatcatt
      541 taaagaaagc gtaatagctc actggtcgag tcggcctgcg cggaagatgt aacggggcta
      601 aaccatgcac cgaagctgcg gcagcgacgc ttatgcgttg ttgggtaggg gagcgttctg
      661 taagcctgtg aaggtgtgct gtgaggcatg ctggaggtat cagaagtgcg aatgctgaca
      721 taagtaacga taaagcgggt gaaaagcccg ctcgccggaa gaccaagggt tcctgtccaa
      781 cgttaatcgg ggcagggtga gtcgacccct aaggcgaggc cgaaaggcgt agtcgatggg
      841 aaacaggtta atattcctgt acttggtgtt actgcgaagg ggggacggag aaggctatgt
      901 tggccgggcg acggttgtcc cggtttaagc gtgtaggctg gttttccagg caaatccgga
      961 aaatcaaggc tgaggcgtga tgacgaggca ctacggtgct gaagcaacaa atgccctgct
     1021 tccaggaaaa gcctctaagc atcaggtaac atcaaatcgt accccaaacc gacacaggtg
     1081 gtcaggtaga gaataccaag gcgcttgaga gaactcgggt gaaggaacta ggcaaaatgg
     1141 tgccgtaact tcgggagaag g
//
LOCUS       DAJOWB010000366         1156 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJOWB010000366 DAJOWB010000000
VERSION     DAJOWB010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.376"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            180..401
                     /locus_tag="OHO85_005105"
     CDS             180..401
                     /locus_tag="OHO85_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcC domain protein"
                     /protein_id="HCQ2674302.1"
                     /translation="MSDPEKYENIYNALIEITNYAQKKSSPCQIIVVDNDVPDKLSDR
                     LKAITVAHYSANKENGLPVGLIDDVIYKH"
BASE COUNT          331 a          273 c          235 g          317 t
ORIGIN      
        1 gaggtttaag aactattatt tccatagggt atatgtcatc aatattaaag agctctattg
       61 actcagacat aaaccatcct cgttttttga tgcttgatac tataggtaag tatctcggaa
      121 aaaacttgaa gacgaaatat gcatccgata ccaatataat agatgatatt gatgaaggaa
      181 tttctgatcc agaaaaatat gaaaacatct ataatgcact catagaaata accaattatg
      241 cgcagaaaaa aagtagcccg tgtcagataa ttgttgtaga caatgatgtc cctgataagc
      301 tttctgacag attaaaagct ataactgttg ctcactatag tgcaaataag gaaaacgggt
      361 tacctgtcgg actaattgat gatgtcattt acaaacatta actactttag attgagggat
      421 gctaaacttt ggttttcact gtgatactta caggaaaacc acagggatgc catcattcca
      481 ttgccgcacc ccagctcgag gatatggcta cctcaggctc tctcataatc ctgcgctccg
      541 gacgaatatc tgttctggtg cagggagctt taatgcccgg aataacattc ctcatatgtg
      601 ccggggagat agtatctgcc atcaggcgag ggtctggttc ctcccggtca accattcatg
      661 ccccgcagac cgctttcccc ggcaagtctg gctccgccgt tggcatcatt tgtacgctac
      721 agtttgcacc cgtcctttga gtcactctgc ccggcataag tcaatgctga aaatgccagg
      781 gtatacagca taaaaaaatc aatatcttac tcatacttta tcctctttcc gcactcatca
      841 tctgaccgtt atcgaatcag cctgctgttc agtcactttc agccccgccc ccgcgagtgc
      901 ggtacagaga gcatccctga tggtcatcct gcctttaacg ggattacccc tgaccggggc
      961 cgtcctgctg aggtctatcc ggataaaaca cccggtccct tgcgccagtt gctgagctgt
     1021 ttcatcaaac cgggaaaact gatatcgtaa ttcgcaatgg tgtaacattt gccttccggc
     1081 acatgagcat ccggggaagc attgcatgaa ataattaaga aaatcgattg accgataaca
     1141 attagactat tttaat
//
LOCUS       DAJOWB010000367         1155 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJOWB010000367 DAJOWB010000000
VERSION     DAJOWB010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.377"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            80..187
                     /locus_tag="OHO85_005106"
     CDS             80..187
                     /locus_tag="OHO85_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674303.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(240..998)
                     /locus_tag="OHO85_005107"
     CDS             complement(240..998)
                     /locus_tag="OHO85_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCQ2674304.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          320 a          276 c          270 g          289 t
ORIGIN      
        1 taataacaaa aaatcccttg atgcctgtcc cttttgttac actccgttat cacgcacaag
       61 agatatgcag gacactggta tgccgactaa acgctttgat aaaaaacact ggaagatggt
      121 ggtggtgcta ctggcaatct gtggcgctat gttgttgcta cgttgggcag caatgatttg
      181 gggctgagca gtgacaaacg aagacagcca gaccgcataa cacggtccgg cagataattt
      241 tacatgctgt agcccagcga taaggttgtc cgacggtcgg tatgttccgg tgcagactct
      301 ggaggttcag agttccatgt gacgttgtaa gccaccttca gtccaaaatg ttcattaata
      361 gcaacattta atgcgctttc ggagttcagc gttgtgtctt ccgcgccaaa gaccgaaaca
      421 ccctgcgtaa atttagcgtt gtcagtcaac tgccatgcat aagccccgga ggcatagccc
      481 agcggctggg tttcactggc attgtcggta tatttgtcgt aacgcacacc tggaccgaat
      541 tcaaagcgga aactgtgtac cgggccattc aaaaactgac gaccataacc cgcggtcaac
      601 acatcgcgct ctcgatagcc gttataacgg tctgtcagcc agcttgcctg accaaataaa
      661 tagtcataat cagttaaatt aaaacggcta cgcccgcccg ccgcatattt ttctgaagaa
      721 cgctcatcat tagaagaagt attactggcg ttcccccaca gcgaccaggc agtggtttgt
      781 ccataccagg tcatggtggt gtcagccgta agtgaggagc ttttcgtatt gcctgattgt
      841 gcaagatatc ctgcgttcag attaccttcg aaaggttttt tagcgctggc aggatcgtcc
      901 atgacagtaa aaacggaatc atcggcagct gcattcagtg acgcaaacat gccccccgcc
      961 aacataacga tggcaggaac tgtcttcaaa agcttcattt atcaagagtc cgtacaacaa
     1021 aaaaagagac catcgcggtc ccggaaactt tcttaaggat caaagattag cgtccctgga
     1081 aaggtaacga attataaaaa ggcgcgaata acttagcaat gtattcttat ttcatttttt
     1141 gaataagcat gtggc
//
LOCUS       DAJOWB010000368         1146 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJOWB010000368 DAJOWB010000000
VERSION     DAJOWB010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.378"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(68..1030)
                     /locus_tag="OHO85_005108"
     CDS             complement(68..1030)
                     /locus_tag="OHO85_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCQ2674305.1"
                     /translation="METGKIALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLF
                     FAHAMWQHFYLNVDRVALSASHATTAGYIILFPSLLASILILKSDLRHKTTLYTINFM
                     LSLCAVIVTETRAAILVFPFFALILIVMDSYINKRINYKLYCFITIALLAGVFSFKDT
                     LLMRMNDLNNDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEK
                     EPRLSGALPYVDSHLHNDLIDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILL
                     FSLLVVGLSDVILFSKPVPTAVFITIILLCAYFKAQSDQCLLEK"
BASE COUNT          407 a          188 c          195 g          356 t
ORIGIN      
        1 tacaactctt taatagataa ttttgaaata aaatcagctt cctcgctgat tttatttcac
       61 atattcatta cttctctaat aaacattggt ccgattgtgc tttaaaataa gcacaaagca
      121 atattatggt gataaacaca gcagtcggaa ccggtttaga aaagagtatt acatcactta
      181 gtcctactac cagtagtgaa aaaagcaaga ttaaaatata aggttcttta gcagttctta
      241 aggcatatat gagtattgct gaaaatgcta aaattgttaa tacaactcca ggaataccac
      301 gcgttgataa cgtatctatg agatcgttat gcaaatgaga atctacatag ggtaaagcgc
      361 cactcaatct aggctctttt tcttctagtt catgtatttt ttccgcacgt ttttccagtg
      421 attgtcctat tggagaatat gtttttaagc caacttcata cattgccaga cgggcaccga
      481 ctgaagttct ggtgttatca tgcgaataat taactaaatc gttatttaag tcattcattc
      541 tcataagcaa tgtatcttta aaagaaaata cacctgctaa taatgcaatc gtaataaaac
      601 aatataactt ataattaatt cgcttattaa tataactatc cattacgatt aatattaacg
      661 caaagaatgg aaacactaat atggctgcac gcgtctcagt tactatgaca gcacataaac
      721 taagcatgaa gttaattgta tataatgttg ttttatgtct taagtcggat tttaaaatga
      781 gaattgatgc cagtaaagaa ggaaaaagga tgatgtagcc tgctgttgta gcgtgggaag
      841 ctgataatgc aacacggtcg acgtttaaat agaaatgttg ccacatggca tgcgcaaaaa
      901 aaagtaactg cgttgccagg gatgcgaaca gaattagttt cccaaatttt atttttgttc
      961 tcatctcatt tttagcaaaa agaactaaga aaataaaagc gctacacaag gcaatttttc
     1021 cagtttccat tgggccacga tatgcattgg tataaacaga gcctgaaatt ttataatgtg
     1081 aataccagat gatatttacg atacccaaaa gacataatga gatgaaaatt aacaaagtag
     1141 gattat
//
LOCUS       DAJOWB010000369         1137 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJOWB010000369 DAJOWB010000000
VERSION     DAJOWB010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.379"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            51..1031
                     /locus_tag="OHO85_005109"
     CDS             51..1031
                     /locus_tag="OHO85_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="HCQ2674306.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGKVKTLKQHPRKNKTAINIEYMKASIRARVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
BASE COUNT          307 a          318 c          296 g          216 t
ORIGIN      
        1 cctgatatga gatcatcata ttcatccgga gcgcatccca gagggacatc atgagccatc
       61 aactcacctt cgccgatagt gaattcagca ctaagcgccg tcagacccga aaagagattt
      121 tcctctcccg catggagcag attctgccat ggcagaatat gaccgctgtc atcgagccgt
      181 tttatcccaa ggcgggcaat ggccgacggc cctatccgct ggagaccatg ctgcgtattc
      241 actgcatgca gcattggtac aacctgagcg acggtgccat ggaagatgcc ctgtacgaaa
      301 tcgcctccat gcgcctgttt gcccgattat ccctggatag cgccctgccg gatcgcacca
      361 ccatcatgaa tttccgccac ctgctcgagc agcatcaact ggcccgtcaa ttgttcaaga
      421 ccatcaatcg ctggctggcc gaagcaggcg tcatgatgac ccaaggcact ttggtggatg
      481 ccaccatcat tgaggcaccc agctctacca agaacaaaga gcagcaacgc gatccggaga
      541 tgcatcagac caagaaaggc aatcagtggc actttggcat gaaggcccac attggtgtcg
      601 atgccaagag tggcctgacc cacagcctgg tcaccaccgc ggccaacgag catgacctca
      661 atcagctggg taatctgctt catggagagg agcaatttgt ctcagccgat gccggctacc
      721 aaggagcgcc acagcgcgag gagctggccg aggtggatgt ggactggctg atcgccgagc
      781 gtcccggcaa ggtaaaaacc ttgaagcagc atccgcgcaa gaacaaaacg gccatcaaca
      841 tcgaatacat gaaagccagc atccgtgcca gggtggagca cccgtttcgc atcatcaagc
      901 ggcagttcgg cttcgtgaaa gccagataca aggggctgct gaaaaacgat aaccaactgg
      961 cgatgttatt caccctggcc aacctgtttc gggtggacca aatgatacgt cagtgggaga
     1021 gatctcagta aaaaccggaa ataacgccag aaatggtgga aaaaatagcc taaataggct
     1081 gattcgatgt gtttgcggga aaaaaatcgg cccagatccg cgaaatttta atcaacg
//
LOCUS       DAJOWB010000370         1124 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJOWB010000370 DAJOWB010000000
VERSION     DAJOWB010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.380"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            197..439
                     /locus_tag="OHO85_005110"
     CDS             197..439
                     /locus_tag="OHO85_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HCQ2674307.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(471..1121)
                     /gene="tetR(A)"
                     /locus_tag="OHO85_005111"
     CDS             complement(471..1121)
                     /gene="tetR(A)"
                     /locus_tag="OHO85_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HCQ2674308.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
BASE COUNT          206 a          370 c          360 g          188 t
ORIGIN      
        1 aagccccgta gcgacgcgga gaggggcgag acaagccaag ggcgcaggct cgatgcgcag
       61 cacgacatag ccggttctcg caaggacgag aatttccctg cggtgcccct caagtgtcaa
      121 tgaaagtttc caacgcgagc cattcgcgag agccttgagt ccacgctaga tctatctcat
      181 ctgcgcaagg cagaacgtga agacggccgc cctggacctc gcccgcgagc gccaggcgca
      241 cgaggccggc gcgcggaccc gcgccacggc ccacgagcgg acgccgcagc aggagcgcca
      301 gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc agggcatgaa
      361 aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag gggatgttgt
      421 ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga tcaatcgtca
      481 ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa tccatcgaca
      541 atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa ggcgtctatc
      601 gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg ctcgccggca
      661 tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct gattgtcatc
      721 agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg aagctgcgcg
      781 tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg cgcgccatcg
      841 cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa tgagcgccag
      901 tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc ttcggccagt
      961 gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg ctgaaccccc
     1021 aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt caacaggtcc
     1081 agggcggcac ggatcactgt attcggctgc aactttgtca tgct
//
LOCUS       DAJOWB010000371         1061 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJOWB010000371 DAJOWB010000000
VERSION     DAJOWB010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.381"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>1061
                     /locus_tag="OHO85_005112"
     CDS             <1..>1061
                     /locus_tag="OHO85_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HCQ2674309.1"
                     /translation="APSAVIQARQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLA
                     IDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSE
                     NELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPLRKGAQYEEIRKLG
                     KGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMGD
                     LYSHRWEIELGYREIKQTMQRSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEH
                     LKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRERAFP
                     RVVKERPWKYPTAPKKSQS"
BASE COUNT          293 a          264 c          315 g          189 t
ORIGIN      
        1 tgcccccagt gccgttattc aggcccgcca gcgcctggga agtgaggctg tccgccgcgt
       61 gttcacgaaa acagcgcagc tctggcataa cgccacgccg catccgcact ggtgcggcct
      121 gaccctgctg gccatcgatg gtgtgttctg gcgcacaccg gatacaccag agaacgatgc
      181 agccttcccc cgccagacac atgccgggaa cccggcgctc tacccgcagg tcaaaatggt
      241 ctgccagatg gaactgacca gccatctgct gacggctgca gccttcggca cgatgaagaa
      301 cagcgaaaat gagcttgctg agcaacttat agaacaaacc ggcgataaca ctctgacgtt
      361 aatggataaa ggttattact cactgggact gttaaatgcc tggagcctgg cgggagaaca
      421 ccgccactgg atgatacctc tcagaaaggg agcgcaatat gaagagatca gaaaactggg
      481 taaaggcgat catctggtga agctgaaaac cagcccgcag gcacgaaaaa agtggccggg
      541 actgggaaat gaagtgactg cccgcctgct gaccgtgacg cgcaaaggaa aagtctgcca
      601 tctgctgacg tcgatgacgg acgccatgcg cttccccgga ggagaaatgg gggatctgta
      661 cagtcatcgc tgggaaatcg aactgggata cagggagata aaacagacga tgcaacggag
      721 caggctgacg ctgagaagta aaaagccgga gcttgtggag caagagctgt ggggtgtctt
      781 actggcttat aatctggtga gatatcagat gattaaaatg gcggaacatc tgaaaggtta
      841 ctggccgaat caactgagtt tctcagaatc atgcggaatg gtgatgagaa tgctgatgac
      901 attgcagggc gcttcaccgg gacgtatacc ggagctgatg cgcgatcttg caagtatggg
      961 acaacttgtg aaattaccga caagaaggga aagggccttc ccgagagtgg taaaggagag
     1021 gccctggaaa taccccacag ccccgaaaaa gagccagtca g
//
LOCUS       DAJOWB010000372         1055 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJOWB010000372 DAJOWB010000000
VERSION     DAJOWB010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.382"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            131..919
                     /locus_tag="OHO85_005113"
     CDS             131..919
                     /locus_tag="OHO85_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674310.1"
                     /translation="MLEKDYQLSAYKKLAAAGGMKTPGAITSARNSANTAKLLAEELT
                     GLILDTIVYPDTITSYVSTIRTTTTGLTNIGELATKHADLLAGYADLSMLLQLDIGWD
                     VYCRANEREVSELPISIAIGDVNITKSLEDAVNALNTSSLVAAMGEINQTLNTGSGSS
                     SGSGSGGGTATPPPALTEEQIESLKVATEQFGVVFNQTTAPTTALQQQYERAKESANV
                     AITAYNHAIGTALAEASANKTSTSSAVAALVPDSVLDELNKAAQ"
BASE COUNT          293 a          244 c          253 g          265 t
ORIGIN      
        1 ggcaagctat atcaaccagg gcatggttta ccgtcaacgg gaaagggatc gtatgcgttg
       61 gtgatcctgt ttcatgcggc tctaccgttg cagccggaga cggcctggtt caggtaagtt
      121 aggagatatc atgctggaaa aagactacca gttatccgca tataaaaaat tggccgccgc
      181 cggtgggatg aaaacacctg gtgccataac atcggcacga aacagtgcta acacagcaaa
      241 actgcttgca gaagaattga ccggattaat tctggataca attgtctatc ccgacactat
      301 taccagctat gtttcaacga tcagaacaac cacaaccggc ttaacgaaca ttggagaact
      361 ggcaactaag cacgcggacc tgttggctgg ttatgcagat ctgtcaatgc tgcttcaact
      421 cgatattggt tgggatgttt actgccgtgc taatgagcga gaagtttcag aactgccgat
      481 ctctattgcc attggtgatg tgaatattac taaatcgctt gaggacgctg ttaacgcgct
      541 taatacatca agtttagtcg ctgctatggg ggagattaac cagaccctta acactggctc
      601 aggaagctcg tcaggctctg gttcaggcgg cggcactgcc actcccccac cagcactaac
      661 agaagagcaa attgaatctc tgaaagtagc aactgaacag tttggggttg ttttcaacca
      721 gacaacagcg cccacaactg cgttacaaca gcagtatgaa cgagcgaagg aaagcgccaa
      781 cgtagccata actgcttata accatgctat cggtaccgcg cttgcggaag catcagcaaa
      841 taagaccagc acatccagcg cagtcgccgc tttggttcct gattctgttc ttgatgaatt
      901 aaacaaagcg gcacagtaac aaaggacttc attgataatt tttcttcagg aggaagacat
      961 gtcattcttt tctacgttaa aaacagcttt gtctttgaag gagaaacttg ctgctactgg
     1021 tgttcttgtt ctgatttgcg cacttgttgg tgctg
//
LOCUS       DAJOWB010000373         1053 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJOWB010000373 DAJOWB010000000
VERSION     DAJOWB010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.383"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..633
                     /locus_tag="OHO85_005114"
                     /pseudo
     CDS             1..633
                     /locus_tag="OHO85_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(678..1052)
                     /locus_tag="OHO85_005115"
     CDS             complement(678..1052)
                     /locus_tag="OHO85_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012304841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCQ2674311.1"
                     /translation="MFRIEPNLIKAIALVESNLKKDSIGKNRDKNNNIKSLDYWLMQI
                     NQMHIPLLKKRGIIKDERDLLDNPCLNIKIGTEILYNHFSRCGVTWQCLGTYNAGFAM
                     DNQKKRQQYAPKYILYIPGLMN"
BASE COUNT          333 a          167 c          198 g          355 t
ORIGIN      
        1 atgggaaaaa ttaaaattgt agtttcagat cagcagccgt ttatgattga tgggataatt
       61 ggatttctcg gacattatcc tgatttatat gaggttgttg ggggctataa agatctgaag
      121 aaatctatag cggagtgtaa caaatctaca gcctaaatat ttattttagg agaatttgca
      181 ggtggaatga tggggtccga actcgtaaaa tgggttaaat cgcataagat agatgctcat
      241 attattacat ttgtagcaaa gatgccgtat attgattcaa taaaattgct tgaagcaggt
      301 gcgaaaggat gcgtatggaa aaccagtcac ccggcgaaac taaatcgtgc tattgattcg
      361 attagtaatg gttacactta ttttgatagt gtacatatgg attgtgaaaa aatatcttcc
      421 aggtattcct ctgataatca acttacaaat cgtgagtcag aaatattaca actcatagct
      481 gatggtaaaa caaacaaaga aatcgctaat ttcttgcagt taagcagaaa aacagtcgaa
      541 actcatagac tcaatatcat gaagaaatta gatgttcaca gtggtatcga gttgatcaaa
      601 acagctttac gtatgggtgt ttgcactata taaaaaaaag aggctttagg ctcattttgc
      661 tgaagcctta cgattgtcta gttcattaag cctggtatat acaatatata ttttggggca
      721 tattgctgtc tctttttttg gttatccatt gcaaaacccg cattataagt ccccaggcat
      781 tgccacgtta ctccacaacg tgaaaaatgg ttatataaaa tctcggttcc tattttaatg
      841 ttcagacaag ggttatctaa caaatctcgt tcatctttta ttattccacg tttttttaac
      901 aggggaatat gcatctggtt tatctgcatt aaccaataat ctaaactctt tatgttgtta
      961 ttcttatctc tgttcttgcc tatgctatcc ttttttagat tgctttcaac aagagcgata
     1021 gctttaatta aatttggttc gattctgaac att
//
LOCUS       DAJOWB010000374         1044 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJOWB010000374 DAJOWB010000000
VERSION     DAJOWB010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.384"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..322
                     /locus_tag="OHO85_005116"
     CDS             <1..322
                     /locus_tag="OHO85_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="relaxase NikB"
                     /protein_id="HCQ2674312.1"
                     /translation="NHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEM
                     VAWHNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEP
                     PSPG"
     gene            complement(359..>1044)
                     /locus_tag="OHO85_005117"
     CDS             complement(359..>1044)
                     /locus_tag="OHO85_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HCQ2674313.1"
                     /translation="WIDSPQISILMESKINVQELIELHPGEFFSIFRGETVPSASFFI
                     PDDEKSCSSDPVVINRYISVDAPRLDRLRRLVPRTTQRRIPSPENVSAIIGVLTAKPS
                     RKRRKIRTEPHTIVDTFQQRIAGRQAAMAMLEEYDTDINARESALWETAVNTLKTTTR
                     EERRIRYITLNRPELPETKEENQISVRAERAGINLLTLPQDNNHPTGRPVNGFHHKKT
                     NRPDWDGMY"
BASE COUNT          237 a          253 c          270 g          284 t
ORIGIN      
        1 taatcaccat gaccgcaatg cgctggcaga taaactggat ttgattgcac cggtattatt
       61 cgggcgtgat ccacgcatgg ggtttgaacc agaaggaaac gacaaacaat ttaaccaggt
      121 ctttgctgaa atggttgcct ggcataatgt gacaggccgt accggtcacg aagactacag
      181 gattacgcga ccggacgtgg atcatcaccg tgaaggaagt gaacgctact accgcgatta
      241 tatcgcggcg aacagcaacg atgacgcatc actgcctccg ccggagcagg ataaacgctg
      301 ggaaccgcct tctccaggtt aaaaaaaagg ccgagccaga atccggcgcg gccactgttc
      361 agtacattcc gtcccagtcc ggacggttgg tttttttatg atgaaacccg tttacaggac
      421 gccctgtcgg gtggttattg tcctgtggaa gcgttaacag gtttatacct gctctttctg
      481 ctctcactga tatctgattt tcctcttttg tttcaggtag ttctggtctg ttcagcgtga
      541 tatagcgaat tcgtcgctct tcccgtgtgg tcgttttaag cgtattcacc gctgtttccc
      601 acaaggcact ttcacgtgcg ttgatatctg tgtcgtactc ttccagcatt gccatagccg
      661 cctgacgtcc tgcaatgcgt tgctgaaatg tatccacgat cgtgtgcggc tctgtgcgaa
      721 tttttcgtct ttttctggag ggttttgcag tcaggacgcc aatgatggca ctgacatttt
      781 ccggtgacgg tattcgacgt tgagtagtac ggggaacaag acggcggaga cggtccaggc
      841 gtggcgcatc cacgctgata tacctgttta taacaacggg atcgctgctg cagcttttct
      901 catcgtcagg gatgaagaat gatgctgatg gtacggtttc tccccggaaa atagaaaaga
      961 actcgccagg atgaagttca atcaactcct gaacatttat cttgctttcc atcaggatgc
     1021 tgatttgtgg gctgtcaatc catg
//
LOCUS       DAJOWB010000375         1024 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJOWB010000375 DAJOWB010000000
VERSION     DAJOWB010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.385"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..267
                     /locus_tag="OHO85_005118"
     CDS             <1..267
                     /locus_tag="OHO85_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674314.1"
                     /translation="RLYEWLNTYQGEVYGDPSWGNVLPQFKHEPTNLSHVQIAVEAML
                     LQKLTVDLPDIPISGLSVAEGDAFDKLKISIRIRDITITQDVVL"
     gene            264..>1024
                     /locus_tag="OHO85_005119"
     CDS             264..>1024
                     /locus_tag="OHO85_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bleomycin hydrolase"
                     /protein_id="HCQ2674315.1"
                     /translation="MSKTTPTKDSIRAEFEELVEKDSFWSKFVGSQFVSMLTLFITQI
                     VYRCFQYADAALAEGFISTATRRSSILAAAETNSYVGTKPTPSSGMIEITATSEDAPA
                     VIPKNMPLISDDQYPYMTMDVCRLVDGTGTVEVAQLEIQEVTYTVTAAKEFLEVVLSK
                     ALTAVCYKLEVFVTTDGKTTQWSSSTMFRLAGSKSQVYVEFYKPSEQLGVRFGDGLIG
                     QIPPEGSTITLKVWCTNGDITLVAGQNLTPVDSAA"
BASE COUNT          269 a          226 c          266 g          263 t
ORIGIN      
        1 cgtttatatg agtggctaaa cacttatcag ggtgaggtat acggagatcc gtcatggggc
       61 aatgttttac cgcagtttaa acacgaaccg accaacttgt cgcatgttca aattgcggtt
      121 gaggcaatgc tgttgcaaaa actgacggta gatttacctg acataccgat ttctggcttg
      181 tcagtagccg agggagatgc ttttgataag ttgaaaatat ccattcgtat cagggatata
      241 actatcacac aggacgtggt gctatgagta aaacaacacc gactaaagac agtattcgtg
      301 cagagtttga agagcttgtc gagaaagatt cattctggtc gaagtttgtc ggctctcaat
      361 ttgtctcgat gctgacattg tttattaccc agattgtcta caggtgcttt cagtatgccg
      421 atgcggcgct ggctgaaggc tttatatcga ccgcgacgcg gcgttcctct atcctggcag
      481 cggcagaaac gaatagttac gttggtacca agccaacacc gtcatcgggg atgattgaga
      541 tcaccgccac aagtgaagat gccccagcgg taatccccaa aaacatgcct ttaatatctg
      601 acgaccagta cccttacatg actatggatg tatgcaggtt ggttgacggc accggtacgg
      661 tagaagtggc acagttggaa atccaggagg tgacatatac cgttacggca gccaaagaat
      721 ttctggaagt cgtgttatca aaggctctca ctgctgtctg ctataagctg gaagtattcg
      781 tgacgaccga tggtaagacc acgcagtggt cttccagcac tatgttccgg ttagccggta
      841 gtaaaagcca ggtctacgtt gagttttata aaccatccga gcagttgggg gttcgattcg
      901 gtgatgggct aattgggcaa ataccgccag aaggctcgac cattacgctt aaggtatggt
      961 gcaccaacgg cgatataacc ctggttgctg gccaaaacct gacgcctgtc gattctgcgg
     1021 ctaa
//
LOCUS       DAJOWB010000376         1007 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJOWB010000376 DAJOWB010000000
VERSION     DAJOWB010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.386"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>1007
                     /locus_tag="OHO85_005120"
     CDS             <1..>1007
                     /locus_tag="OHO85_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HCQ2674316.1"
                     /translation="AESWEDIEDFGETHLDFLKQYGDFENDIPVHDTIARVVSCISPA
                     KFHECFINWMRDCHSSDDKDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVIGQ
                     IKTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQG
                     RLNKAFEEKFPLKELNNPAHDCSAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLK
                     KLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVV
                     MNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAG
                     S"
BASE COUNT          332 a          178 c          242 g          255 t
ORIGIN      
        1 gtgcagaaag ttgggaagat atagaggatt tcggggaaac acatctcgat ttcttgaagc
       61 aatatggtga ttttgaaaat gatattcctg ttcacgatac tattgccaga gttgtatcct
      121 gtatcagtcc tgcaaaattt catgagtgct ttattaactg gatgcgtgac tgccattctt
      181 cagacgataa agacgtcatc gcaattgatg gaaaaacgct ccggcactct tatgacaaga
      241 gtcgccgcaa gggagcgatt catgtcatta gtgcgttctc aacaatgcac agtctggtca
      301 tcggacagat caagacggat gagaaatcta atgagattac agctatccct gaacttctta
      361 acatgttaga tattaaagga aaaattatca caactgatgc gatgggttgc cagaaagata
      421 ttgcagagaa gatacaaaaa cagggaggtg attatttatt cgctgtaaaa ggaaaccagg
      481 ggcggcttaa taaagccttt gaggaaaaat ttccgctgaa agaattaaat aatccagcgc
      541 atgactgttc cgcaattagt gaaaagagtc acggcagaga agaaatccgt cttcatattg
      601 tttgcgatgt ccctgatgaa cttattgatt tcacgtttga atggaaagga ctgaagaaat
      661 tatgcgtggc agtctccttt cggtccataa tagcagaaca aaagaaagag ccagaaatga
      721 cggtcagata ttatatcagt tctgctgatt taactgcaga aaagttcgcc acagcaatcc
      781 gaaaccactg gcacgtggag aataagctgc actggcgtct ggacgtggta atgaatgaag
      841 acgactgcaa aataagaaga ggaaatgcag cagaattatt ttcagggata cggcacattg
      901 ctattaatat tttgacgaat gataaggtat tcaaggcagg gttaagacgt aagatgcgaa
      961 aagcagccat ggacagaaac tatctggcgt cagtccttgc ggggagc
//
LOCUS       DAJOWB010000377         1007 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJOWB010000377 DAJOWB010000000
VERSION     DAJOWB010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.387"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            13..579
                     /locus_tag="OHO85_005121"
     CDS             13..579
                     /locus_tag="OHO85_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674317.1"
                     /translation="MTPRQLLEDVKSRFTPLIADEPALLESLLRKALGTYQDRAGHIK
                     RIRFTDQTCKSLACPADFLALVSVTDHTGDLVYSDVYDGNIELEDTHRAVYPLNVSYL
                     ANLRDMDLDNGEVPPEIIGLLSDYLEVLIAIPNTDRLRRISIAGKLDASNLSDENTLY
                     QRKLDLEEKMSATRAIIPGIVLFSSMLK"
     gene            590..>1007
                     /locus_tag="OHO85_005122"
     CDS             590..>1007
                     /locus_tag="OHO85_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2674318.1"
                     /translation="MGLNVASVKSYVSSALTTTLFGSGVGEREVGKLTSIIMNKMLFA
                     QGWQFSVEVDGLEGADFFAKDITYHDYSIEYETIKIGGGNILQPTERSPGQITMMVRD
                     TVDGLVLDWFKTAKSRVINPDGTGNIPSKYLLNVRIY"
BASE COUNT          269 a          216 c          257 g          265 t
ORIGIN      
        1 tacgaggttt caatgacacc acgacaatta ctcgaagacg tcaaatcccg cttcacacct
       61 ttgattgcgg atgaacctgc cttactggaa tccctgctaa gaaaagcatt gggaacctac
      121 caggataggg cgggacacat caagcggata cgcttcactg atcagacctg taaatcactt
      181 gcttgccctg ctgattttct tgcgctcgta tcggttacgg atcataccgg cgatcttgtc
      241 tactccgatg tttacgatgg gaatatcgag cttgaagata ctcatcgagc ggtatacccg
      301 ctgaatgtgt catatctggc taatttacgt gatatggatc tggataatgg ggaagtgcca
      361 cctgaaatca ttgggttact ttctgactat ctggaagtgt taatcgcgat acctaacact
      421 gatcgcctgc gaagaatatc tatcgcgggg aaactcgatg ccagcaattt atccgacgag
      481 aacacgctgt atcagcgaaa gctggatctg gaagagaaaa tgagcgcaac aagggcaatt
      541 atcccgggga ttgttctttt ctcatccatg ttgaagtgag ggggctgata tggggcttaa
      601 tgttgcttca gtaaagtctt atgtatcttc ggcattaacg acgacattat ttggctccgg
      661 cgttggtgag cgggaagttg gtaagctgac gtcaatcatc atgaacaaaa tgctgttcgc
      721 gcaaggatgg cagttctctg tcgaagttga tggactggag ggggcagact tctttgccaa
      781 agacattacc taccacgatt acagcatcga atatgaaacg attaaaatcg gcggagggaa
      841 tatccttcaa ccaacggagc gttcgcctgg gcagataaca atgatggtca gggataccgt
      901 tgatggcctc gttttggact ggtttaagac ggcaaaaagt cgggtgatca atccggacgg
      961 taccgggaat ataccgtcta aatatttgct caatgtgcgt atttatc
//
LOCUS       DAJOWB010000378         1003 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJOWB010000378 DAJOWB010000000
VERSION     DAJOWB010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.388"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(136..366)
                     /locus_tag="OHO85_005123"
                     /pseudo
     CDS             complement(136..366)
                     /locus_tag="OHO85_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937477.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            423..>1003
                     /locus_tag="OHO85_005124"
     CDS             423..>1003
                     /locus_tag="OHO85_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCQ2674319.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEG"
BASE COUNT          305 a          217 c          188 g          293 t
ORIGIN      
        1 gatattactt taatcaaaag ttgacgtgcc agattcgaag taaatttata aaatatcaaa
       61 tcaatataca ttttgcacct tcgagatagt aatgactgtt actgtaacat gagttacaaa
      121 cagcttcata cccagagcta ctgatgtatc aactcgattc aacaataccc gatatttatt
      181 ccatgcctcc agcaacgatc tttcttcctc cgttgcgatt tccagatcta cagcatcctg
      241 cagtggcgca atatactcac tgaattcctg gatgtagaac tgtgtagtga cggtcttcca
      301 gccattcggc tcctgctgta tcgaagcata ccaggctatt tcaatatcgc tatgctgcgg
      361 cagcatttaa ccccttgtaa ttcatcgcca taattgattt aattcacaaa taaaactata
      421 acatggtgaa attaatgaaa aaaaacacag atgatggggc taaaatttac acaccactta
      481 ccctaaagct ttatgactgg tgggttttgg gagtatcaaa tcgtcttgca tggggatgtc
      541 ctacaaagga acaccttctt ccacactttc tggaacattt aggtaacaac catctggata
      601 ttggtgttgg aactgggttt taccttactc acgtacctga gagtagtctg atatctttaa
      661 tggatttgaa cgaagctagc ctgaacgcgg catctacaag ggctggggaa tcaaaaatta
      721 aacataaaat tagccatgat gtttttgaac cttatcccgc ggcgttacat ggtcaatttg
      781 attccatttc catgttttac cttcttcact gcctgcctgg aaatatatct acaaaaagct
      841 gtgtaatacg caatgcggcg caggccttaa ctgacgatgg aactctatac ggagccacaa
      901 ttcttggcga tggagttgtg cacaatagct tcggtcaaaa actgatgcgc atttacaatc
      961 agaaaggcat cttttcaaac acaaaagatt ccgaagaagg ctt
//
LOCUS       DAJOWB010000379          990 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJOWB010000379 DAJOWB010000000
VERSION     DAJOWB010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.389"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..990
                     /locus_tag="OHO85_005125"
                     /pseudo
     CDS             1..990
                     /locus_tag="OHO85_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    149..265
                     /locus_tag="OHO85_005125"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          233 a          237 c          307 g          213 t
ORIGIN      
        1 agactctgcg tgtctgggta cgccagcatg agcgggatac cggaggcggt gatggcgggc
       61 tcaccaccgc tgaacgtcag cgtctgaaag agctggaacg cgaaaatcgt gaactgcgcc
      121 gcagtaacga tatccttcgc caggcttccg cttattttgc gaaggcggag ttcgaccgcc
      181 tctggaaaaa gtgatgccac tgctggataa gctgcgtaag ctgtacgggg tcggaccggt
      241 atgcagtgaa ctacatattg ccccgtcaac gtattaccac tgtcagcaac agcgacatca
      301 tccttataaa cgcagtgccc gtgcgcagcg cgatgactgg ctgaagaaag agatactgcg
      361 cgtatacgat gggaatcatc aggtatacgg tgtgcgtaaa gtctggcgtc agttgttacg
      421 ggaaggaatc agggtagcca gatgtacagt ggcgcgcctc atggcggtta tgggacttgc
      481 cggtgttctc cggggtaaaa aggtccgcac taccgtcagc cggaaagccg ttgccgcagg
      541 cgaccgcgta aaccgtcagt ttgtggcaga acgtcctgac cagttgtggg tggctgattt
      601 tacttacgtc agcacatggc agggggtcgt ctatgtggcg ttcatcattg atgtgtttgc
      661 cggatacatc gtggggtggc gggtctcatc gtctatggaa acgacattcg tgctggatgc
      721 tctggagcag gcgttatggg cccgtcgacc gtccggcaca gtccatcaca gtgataaagg
      781 ttctcagtat gtatcgctgg cctacacaca gcggcttaag gaagccggat tactggcatc
      841 aacaggaagt actggtgact cgtatgacaa cgcgatggcg gagagcatta atggccttta
      901 caaagcggag gtaatacacc gtaagagctg gaaaaaccgg acagaagtgg agctggccac
      961 actcacgtgg gtggactggt ataacaatcg
//
LOCUS       DAJOWB010000380          986 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJOWB010000380 DAJOWB010000000
VERSION     DAJOWB010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.390"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..142
                     /locus_tag="OHO85_005126"
     CDS             <1..142
                     /locus_tag="OHO85_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2674320.1"
                     /translation="LKKALQTQANNLIKFFMSQGMDKAAATAQARSILETQINEARKR
                     KG"
     gene            146..874
                     /locus_tag="OHO85_005127"
     CDS             146..874
                     /locus_tag="OHO85_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2674321.1"
                     /translation="MAGFFDDMFEDTEPSQQVTGDNLPDTESDPDIPGEGSELIEEED
                     IDAEIETDGVNVGNIVDPVEDNHLPNLDHGLLSDSGVRHRYQGHAVFNNLVRMDWLKA
                     IKLDPDSFDAVLYRAIPYRDKNAPETASEIIEPNQRIYDYQDPELITALDCPDEMDAF
                     YALYDGSDNTGISDSALILRLAAVNVPVGSMLEWLEQLSDGTTIRRFWYIHKIFNYGT
                     ARVGSLFYCVPSRAFEGNFIGDSE"
BASE COUNT          279 a          229 c          238 g          240 t
ORIGIN      
        1 tctcaaaaaa gccctgcaaa cgcaggcgaa caacctcata aagtttttca tgagtcaggg
       61 aatggacaag gccgctgcca ctgcgcaggc gcgaagcatt cttgagacac aaattaacga
      121 agcccgtaaa cggaaaggat aatcgatggc tggtttcttt gatgacatgt ttgaggacac
      181 agaaccatca caacaagtga ctggtgataa cctcccggac accgaatcgg atccggatat
      241 tccaggcgaa ggttctgaac tgattgaaga ggaagatatt gatgctgaaa tcgaaaccga
      301 tggtgttaac gttggtaata ttgttgatcc tgtggaggac aatcaccttc ccaatctgga
      361 tcacggcctg cttagtgatt ctggtgtgcg ccaccgttat caaggtcatg cagtttttaa
      421 taaccttgtg cggatggact ggctcaaagc aatcaagcta gaccctgact cattcgatgc
      481 agttctgtat cgcgcaatac cttacagaga caaaaatgca cctgaaacgg catctgaaat
      541 aatagaaccg aaccaacgca tatatgacta tcaggatcca gaactgataa cggccctcga
      601 ctgcccggat gagatggacg ccttctacgc gctatacgac ggcagtgata atacgggaat
      661 tagcgacagt gctttaatcc ttcggttagc cgccgtcaat gtgccagtgg gttctatgct
      721 cgaatggctg gaacagctgt cagacggcac aaccattcgc cgcttctggt acatccataa
      781 aatattcaat tacggcactg ccagggtagg cagtttgttt tattgcgtgc cttcacgcgc
      841 ctttgaaggg aatttcatcg gtgattctga ataatcagga atggctactg gccatcttta
      901 agaaaaaagg tcttactcca accggtaagc tggaatttgc cactattgat ggcattgatt
      961 cggcgctcgc acaggcttta aacgaa
//
LOCUS       DAJOWB010000381          978 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJOWB010000381 DAJOWB010000000
VERSION     DAJOWB010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.391"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..483
                     /locus_tag="OHO85_005128"
     rRNA            <1..483
                     /locus_tag="OHO85_005128"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            569..644
                     /locus_tag="OHO85_005129"
     tRNA            569..644
                     /locus_tag="OHO85_005129"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:603..605,aa:Glu,seq:ttc)
     gene            838..>978
                     /locus_tag="OHO85_005130"
     rRNA            838..>978
                     /locus_tag="OHO85_005130"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          257 a          223 c          272 g          226 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcacctcc
      481 ttaccttaaa gaagcgttct ttgaagtgct cacacagatt gtctgataga aagtgaaaag
      541 caaggcgtct tgcgaagcag actgatacgt ccccttcgtc tagaggccca ggacaccgcc
      601 ctttcacggc ggtaacaggg gttcgaatcc cctaggggac gccacttgct ggtttgtgag
      661 tgaaagtcac ctgccttaat atctcaaaac tcatcttcgg gtgatgtttg agatatttgc
      721 tctttaaaaa tctggatcaa gctgaaaatt gaaacactga acaacgagag ttgttcgtga
      781 gtctctcaaa ttttcgcaac acgatgatga atcgaaagaa acatcttcgg gttgtgaggt
      841 taagcgacta agcgtacacg gtggatgccc tggcagtcag aggcgatgaa ggacgtgcta
      901 atctgcgata agcgtcggta aggtgatatg aaccgttata accggcgatt tccgaatggg
      961 gaaacccagt gtgattcg
//
LOCUS       DAJOWB010000382          971 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJOWB010000382 DAJOWB010000000
VERSION     DAJOWB010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.392"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(211..861)
                     /gene="zinT"
                     /locus_tag="OHO85_005131"
     CDS             complement(211..861)
                     /gene="zinT"
                     /locus_tag="OHO85_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCQ2674322.1"
                     /translation="MAIRLYKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT          272 a          183 c          198 g          318 t
ORIGIN      
        1 ggcgtgagca caggtatctt tctctgttgg ccgtattgtg tatggaatcc gttattggtt
       61 aactcaatgc gggttagctt attaatatac agacacgtca tagtgatgag cgtgtctgta
      121 gctagcttgc gttcagtggc gtttgtggat ggaccagtgg cagttatgat aacttactaa
      181 agcggcatcg aggcgttatc atgagaatac tcaatgagac atcatttcct cgaccacttc
      241 ttcgctactc aactgatatg gataatacgt tggccagttt tccatttcat ttaataaaga
      301 ttgctgtgaa tcattaccca taaaaatgtg aaaatgagat gattttcgtg gtgcaattat
      361 atgatcgcta aattgtatat atttaggggc tttgctttca ggatctttac attcgaataa
      421 gtaacgaacg cctttcttgc ctgatttata ggtgagtatt ttgtatccat cgtaatcata
      481 tttacacgat gttgtttcat tatttctatg gaattcaaca atgccgtcct caatgccaat
      541 catctcgata tctgttgcat aacctttgtg ataataatct ttaatttcag caaatgtttt
      601 agttttatct gcatccgctt tcttctgaaa gacggggtca agtttgccac tttgcagtaa
      661 aggataaacg gattgccaga ctccatccca gtcactgagc gttcggtttt gtacattggc
      721 atcatcaaaa acaccattag cagctttttg ttcgacctct gttaagggtt tgccgtgtga
      781 gtgatgacca tgcgaaaagg caggagcgct aacaataaag acacctaaag caacagccag
      841 tttgtaaaga cgaatcgcca aaacagtttc ctccagagtt aatgtatatg atgtgtaatg
      901 ttatattgta acatatagca aaatgcaaca actcttagga aacgagagca tggctttctc
      961 agttctactt a
//
LOCUS       DAJOWB010000383          967 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJOWB010000383 DAJOWB010000000
VERSION     DAJOWB010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.393"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..380
                     /locus_tag="OHO85_005132"
     CDS             <1..380
                     /locus_tag="OHO85_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCQ2674323.1"
                     /translation="PLFCLFSGYHFYRERFAERGFFYKVPAVLRDYLSAIPVKINEKA
                     RYKPGIASYHNIITCGFSTLSPYIRQQPLAMQQRFNLLFPDFVDHIQLPLPLASTLLE
                     RITFYAKKNRDELDKISCKWCCD"
     gene            373..594
                     /locus_tag="OHO85_005133"
                     /pseudo
     CDS             373..594
                     /locus_tag="OHO85_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304051.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            597..>967
                     /locus_tag="OHO85_005134"
     CDS             597..>967
                     /locus_tag="OHO85_005134"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674324.1"
                     /translation="MINDLKSILLKSSEEVDVFIKIFESWRKKLPAISGPVNLYIPTR
                     FKDKYLEVESYFVDKSIWNVHITFHDDKRFVFFTDQFIAEFSPQEFVDNCEQYLINNH
                     CFSPDKVNEICEQARHYLVEK"
BASE COUNT          334 a          143 c          170 g          320 t
ORIGIN      
        1 ttccattgtt ctgtttattc agtggttatc acttttatcg agaaaggttt gcagaacgcg
       61 gattttttta taaggttcct gctgtgctaa gggattattt atcagctata ccggtgaaaa
      121 taaatgaaaa ggcacgatac aaacccggca tcgctagcta tcacaatatc atcacatgtg
      181 gtttttcaac gttgtcaccc tatataaggc aacaaccatt ggcaatgcag caacgattta
      241 atttattatt tcctgatttt gttgatcaca tacaattgcc gttaccactt gcatcaactc
      301 ttttagagag aattacattt tatgcgaaaa aaaatagaga tgagcttgat aaaatctcct
      361 gcaaatggtg ttgtgattaa aagaaaaatt tctgatggat tgaaataaat agtttctctt
      421 aaagaaagta tccttcttga aaccagcgct aaaattcaga gcattgaggt aaagatagaa
      481 gaaaaattta ttcaaggtta ttatgatggc tatacaaaag gcattattga tgtgatggat
      541 aattttatac ccttgataag cttgttgagc tcagaactag aaaaaaaaga ataaacatga
      601 taaacgatct taagagtatt ttgttaaaat cctcagagga agttgatgtt tttatcaaaa
      661 tttttgaaag ttggagaaaa aaattaccgg caatatcagg tcctgtaaat ctctatatac
      721 ctaccagatt caaagataag tatcttgaag tagagtcata ttttgtcgac aaatcaatat
      781 ggaatgtcca tattacattt catgatgata agcgttttgt gttttttacc gatcaattta
      841 ttgctgaatt tagtccgcag gaatttgtgg ataattgtga gcaatattta attaataatc
      901 attgtttctc ccctgacaag gttaacgaaa tctgcgaaca agccagacat tatttagttg
      961 aaaaaat
//
LOCUS       DAJOWB010000384          950 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJOWB010000384 DAJOWB010000000
VERSION     DAJOWB010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.394"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            35..820
                     /locus_tag="OHO85_005135"
     CDS             35..820
                     /locus_tag="OHO85_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCQ2674325.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          315 a          189 c          206 g          240 t
ORIGIN      
        1 ctaatcatta tgacaggcga gggagtgtcc aattatgaat tcaaaaaagc tttgttgcat
       61 atgtgtgtta ttctcgctgc ttgcaggatg tgcctctgaa tcttctattg atgaaaagaa
      121 gaaaaaagct caagtcacac aaagtaatat taataaaaac actccccagc aactgacaga
      181 caaagattta ttcggtaatg aaaccactct ggccgtatcc gaagaggata ttcaagctgc
      241 gcttgatgga gatgagttcc gcgttcccct taattctcct gtaatacttg ttcaatccgg
      301 caaccgcgca ccggaaacca ttatgcagga ggagatgcga aaatattata ctgtttccac
      361 attctctggt atcccggaca ggcaaaagcc tctgacttgt aacaaaaaca aagataaaaa
      421 tgagaacgag gatgttgcca gtgctgagaa tatgaactgg atgcaggcac tgcgttttgt
      481 ggctgcaaaa ggacatcaga aagcgattat tgtttaccag gatacattgc agacaggaaa
      541 atatgactct gcgctgaaat caacagtatg gtccgactat aaaaatgaaa aactcaccga
      601 cgctatctcc ctgcgctacc tggtacgttt cacgctggtg gatgtggcaa caggtgagtg
      661 ggctacctgg tcgccggtga attatgaata taaagtgctt ccaccattgc ccgacaagaa
      721 tgaagccagt acgactgata tgacagagca gcaaatcatg caacttaagc aaaagaccta
      781 taaagcgatg gtaaaagatt tggttaaccg ctatcaataa taaattatat ctgccgccag
      841 gaatttctgg cggcaataat acaaaattct ttgcataaga aatttttctt gacaaagaca
      901 gtgcaagaaa ggacatggaa tccggagtta aaaactgttt gttttagaat
//
LOCUS       DAJOWB010000385          943 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJOWB010000385 DAJOWB010000000
VERSION     DAJOWB010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.395"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..567)
                     /locus_tag="OHO85_005136"
     CDS             complement(<1..567)
                     /locus_tag="OHO85_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCQ2674326.1"
                     /translation="MPRLTAPRRQAEVCAPLPAAHAAARYARHLPERTLLYALVQAHY
                     PDFIARLEAEDRPRPEYVREEFETYLRCGVLEHGFLRVVCEQCRAERLVAFSCKKRGF
                     CPSCGARRMAESARHLVEEVFGPRPVRQWVLSFPYPLRFLFASKPEAIGPVLGIVHRV
                     IAGWLADQAGVDHASAQCGAVTLIQRFGS"
     gene            complement(756..>943)
                     /gene="groEL"
                     /locus_tag="OHO85_005137"
     CDS             complement(756..>943)
                     /gene="groEL"
                     /locus_tag="OHO85_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411226.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; many bacteria have multiple
                     copies of the groEL gene which are active under different
                     environmental conditions; the B.japonicum protein in this
                     cluster is expressed constitutively; in Rhodobacter,
                     Corynebacterium and Rhizobium this protein is essential
                     for growth; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCQ2674327.1"
                     /translation="FGDMIEFGILDPTKVTRSALQNAASIAGLMITTEAMVAEAPKKE
                     EPAAPGGGMGGMGGMDF"
BASE COUNT          145 a          333 c          322 g          143 t
ORIGIN      
        1 gctgccgaag cgctggatca gggtcaccgc gccgcattgc gcgctggcgt ggtcgacgcc
       61 ggcttgatcg gccaaccagc cggcgatcac acgatgcacg atgcccagca ccgggccgat
      121 ggcctccggc ttgctggcga acaggaagcg cagcgggtac ggaaaactca gcacccattg
      181 ccgcaccggc cgcgggccaa acacctcctc caccaggtgc cgcgcgctct cggccatgcg
      241 ccgcgccccg cagctggggc agaagccgcg cttcttgcag gagaaggcca ccagcctctc
      301 ggcccggcac tgctcgcaca ccacgcgcag gaaaccgtgc tcgagcacgc cgcaacgcag
      361 gtaggtctcg aactcctcgc gcacatactc gggccgcggg cggtcttcgg cctcaagacg
      421 cgcgatgaag tccgggtagt gcgcctgcac tagcgcgtac agcagcgtgc gctcgggcag
      481 gtggcgcgcg taacgcgcag cggcgtgggc ggccggcagt ggcgcgcaca cctcggcctg
      541 ccgccggggt gcggtcaggc gcggcacgca gcgctccgtt gcagggacgg ctgctcaggg
      601 ttgcgcccgc gagcacgcgt tcatatcggc gttttctgat cttcgcgtca gacattgccg
      661 cggggcgggt aggaagtctc ccgcctcgga cgccagaacg cacaaagccc ggcacgaggc
      721 cgggctttgt ggtcttgctt gatggatcgc ggggcttaga aatccatgcc gcccatgccg
      781 cccatgccgc cgcccggagc ggccggctct tccttcttcg gcgcttcggc caccatcgct
      841 tcggtggtga tcatcaggcc ggcgatggac gccgcgttct gcagcgcgga gcgcgtgacc
      901 ttggtcgggt ccaggatgcc gaactcgatc atgtcgccga act
//
LOCUS       DAJOWB010000386          934 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJOWB010000386 DAJOWB010000000
VERSION     DAJOWB010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.396"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            57..>934
                     /locus_tag="OHO85_005138"
     CDS             57..>934
                     /locus_tag="OHO85_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HCQ2674328.1"
                     /translation="MLAEYERRKGILDTRLSELEKNGGAALAVLDAQQARLLGQQTRN
                     DRAISEARNKLSSVTESLKTARNALTRAEQQLTQQKNTPDGKTIVSPEKFPGRSSTNH
                     SIVVSGDPRFAGTIKITTSAVIDNRANLNYLLTHSGLDYKRNILNDRNPVVTEDVEGD
                     KKIYNAEVAEWDKLRQRLLDARNKITSAESAVNSARNNVSARTNEQKHANDALNALLK
                     EKENIRSQLADINQKIAEEKRKRDEINMIKDAIKLTSDFYRTIYDEFGKQASELAKEL
                     ASVSQGKQIKSVDDAL"
BASE COUNT          315 a          192 c          234 g          193 t
ORIGIN      
        1 aacaggctga aaaacggcag aaggatgcgc agaacgcaga caagaaggcc gctgatatgc
       61 ttgctgaata cgagcgcaga aaaggtattc tggacacgcg gttgtcagag ctggaaaaaa
      121 atggcggggc agcccttgcc gttcttgatg cacaacaggc ccgtctgctc gggcagcaga
      181 cacggaatga cagggccatt tcagaggccc ggaataaact cagttcggtg acggaatcgc
      241 ttaagacggc ccgtaatgca ttaaccagag ctgaacaaca gctgacgcaa cagaaaaaca
      301 cgcctgacgg caaaacgata gtttcccctg aaaaattccc ggggcgttca tcaacaaatc
      361 attctattgt tgtgagtggt gatccgagat ttgccggtac gataaaaatc acaaccagcg
      421 cggtcatcga taaccgtgca aacctgaatt atcttctgac ccattccggt ctggactata
      481 aacgcaatat tctgaatgac cggaatccgg tggtgacaga ggatgtggaa ggtgacaaga
      541 aaatttataa tgctgaagtt gctgaatggg ataagttacg gcaacgattg cttgatgcca
      601 gaaataaaat cacctctgct gaatctgcgg taaattcggc gagaaataac gtcagtgcca
      661 gaacaaatga acaaaagcat gcaaatgatg ctcttaacgc cctgttgaag gaaaaagaga
      721 atatccgtag ccagcttgct gacataaatc agaaaatagc tgaagagaaa agaaaaaggg
      781 atgaaataaa tatgataaag gatgccataa aactcacctc tgatttctac agaacgatat
      841 atgatgagtt cggtaaacaa gcatccgaac ttgctaagga gctggcttct gtatctcaag
      901 ggaaacagat taagagtgtg gatgatgcac tgaa
//
LOCUS       DAJOWB010000387          932 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJOWB010000387 DAJOWB010000000
VERSION     DAJOWB010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.397"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            169..831
                     /gene="excA"
                     /locus_tag="OHO85_005139"
     CDS             169..831
                     /gene="excA"
                     /locus_tag="OHO85_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HCQ2674329.1"
                     /translation="MKDKKETPDRFPTWWLLYYVLRKAYFFLGIPFFLFCALTSTLML
                     FSSRYYGDNIEDYVVTFGSWFLLLAPGIWMYSRAKTRREKIRKVVQTIKESGFYSPEK
                     GYEGLSLTQGAYFGIDLKNGTMLYVRIYPGNIMDVIGFDIHNFTRTVTDDKTLEIHTK
                     YINLPMVPIPSWCTHPETASNTMHAMASRGYDYPVDFPRLIQEKRKEWEQIAGMPVAE
                     VF"
BASE COUNT          301 a          171 c          191 g          269 t
ORIGIN      
        1 aaatatttat tggaggaagt cgcaatatcc gacatactcc tggaatgaaa acagatcgtc
       61 ttaaagataa aggaagtgac gacaaggacg gaataaaagg ataatataaa tattgcacaa
      121 cctccccatc ggcattaaaa taatagatag tacgagggga ggcattgtat gaaagataaa
      181 aaagagacac ctgacagatt tccaacttgg tggttgttat attatgtatt aaggaaggct
      241 tacttctttt taggaatacc tttcttttta ttttgcgcac tgacatctac actgatgtta
      301 tttagttctc gttattatgg tgacaacatt gaagattatg ttgtcacttt tggtagttgg
      361 ttcttattac ttgcgccagg tatatggatg tattcacgtg caaaaacaag gagagaaaag
      421 ataagaaaag ttgtacaaac aataaaagag tcaggcttct actctccaga gaaggggtat
      481 gaaggattat ctttaactca gggagcttat ttcggtattg accttaaaaa tggcacaatg
      541 ttgtatgtcc gtatctatcc aggcaacatt atggatgtta tcggttttga cattcataat
      601 ttcactcgta ctgtaacaga cgacaaaaca ctggaaattc atacgaaata catcaacctg
      661 ccaatggttc ctattccatc atggtgtact catccagaaa ccgcatcaaa caccatgcac
      721 gctatggcaa gtcgaggata tgattaccct gtcgactttc ctcgcctgat tcaggagaag
      781 cgtaaggagt gggagcagat tgcaggcatg cctgtagccg aagtatttta agataagctc
      841 cctccataac agccggttca aatgagccgg cttttgttca ttacaacaga taacttatgc
      901 cgttaccgat aaattcgacc aagtgcttta at
//
LOCUS       DAJOWB010000388          923 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJOWB010000388 DAJOWB010000000
VERSION     DAJOWB010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.398"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          276 a          166 c          183 g          298 t
ORIGIN      
        1 tttgtatttt catttcggaa tactgttaag taactttgat gaccctaaca atagatcgga
       61 attttcttag agtcaatggt cactgttttt gcttgcaaag caaatgtttt ggtggttata
      121 agttgtctta atatagtcac gcatgcaact tgttccatgc ggagattaaa gattggaata
      181 taacgcgaag atggaattaa attacatttg caataatcaa tttattatta ctcaataatt
      241 accgatatta ttattgtaaa ttaatcatta gagtgcaaaa agaaatgtaa cggcaaaata
      301 agaggacgat agctgagaaa aaagcgtgct ttcccaatct ggggcaaatc acgtctgaat
      361 gacacctgaa aacaggtggt agcctcaaga aggcttggaa tattcttctt taatgttatg
      421 taattcattg atttttcgtg tacgatttta agacatttat tccaagaaaa atttttaact
      481 ctttgatttt tcgactctgt atcatcggtc tcgaaaaccg gagtaggggc aactctaccg
      541 ggggttcaaa tccccctctc tccgccactt tatcaatgac ttatctcccg acttcccgcc
      601 ttgcttttcc taaacagaac aatcgtagaa tattcttgaa gggttagatc gtcactgttt
      661 tctgttcgat actgtgacat tcagcacttg attcgctatg gatctgacag gaagctttcg
      721 agcgaaaatg tgcagttatt cagtcgtttt cttatcgctc accattattc ttttagacat
      781 tcatcctatg aaactgccgc aaagttggtg gtgggaactg aagttgcgta gagaaggggt
      841 caataattga gtcaaaaaag gaacgcggcc gtcagattaa agccgcggga caaagtccat
      901 aatttccaag agttgttgaa ttt
//
LOCUS       DAJOWB010000389          922 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJOWB010000389 DAJOWB010000000
VERSION     DAJOWB010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.399"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            91..>922
                     /locus_tag="OHO85_005140"
     CDS             91..>922
                     /locus_tag="OHO85_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbC"
                     /protein_id="HCQ2674330.1"
                     /translation="MSEHRVNPELLHRTAWGNPVWNALQSLNIYGFCLVASLVASFIW
                     PLALPACLLFTLITMLVFSLQRWRCPLRMPMTLECADPSQDRMIKRSLFSFWPTLFQY
                     EVILESPASGIFYVGYQRVRDIGRELWLSMDDLTRHIMFFATTGGGKTETIFAWAINP
                     LCWARGFTLVDGKAQNDTARTIWYLARRFGREDDVEVINFMNGGKSRSEIILSGEKTR
                     PQSNTWNPFCYSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     T"
BASE COUNT          230 a          226 c          240 g          226 t
ORIGIN      
        1 ccgacaccgg ctggcatctg cctacaggaa ttctgcagga aactggcacg cttaatttat
       61 tcctgaaaac caccgacagc gagtctggcc atgagtgaac atcgcgttaa ccccgagctt
      121 ttacatcgta cagcctgggg aaatcctgtc tggaatgcac tccagagtct caatatctat
      181 ggcttttgcc tggtggcaag tcttgtggcc agttttatct ggccacttgc attaccggca
      241 tgtctgttgt tcacactgat aacaatgctg gtatttagtc tgcagcgatg gcgctgccct
      301 cttcgtatgc ccatgacgct ggaatgtgct gacccgtcac aggacagaat gattaaaaga
      361 agtcttttca gcttctggcc aaccctgttc cagtatgagg tcattctgga atcaccggcc
      421 agcggcatat tctatgttgg ttatcaacgc gtcagggata ttggcaggga gttatggctg
      481 agtatggacg atctgacccg ccatataatg tttttcgcca ccaccggtgg cggtaaaacg
      541 gaaaccatct ttgcctgggc gattaaccca ctttgctggg caagaggatt cacccttgtc
      601 gatggcaagg cacagaatga tacggcaaga acgatctggt atctggccag acgctttgga
      661 cgtgaagacg atgtggaagt cattaacttc atgaacggag gaaaatcccg cagtgaaatc
      721 attctgagtg gtgaaaaaac gcgtcctcag tcaaatacct ggaatccctt ctgttacagc
      781 accgaggcat tcacagcgga gacaatgcag tcaatgctgc cccagaacgt ccaggggggc
      841 gagtggcagt cccgggccat tgccatgaat aaggcactgg tatttggtac taaattctgg
      901 tgtgtgcgcg aaggcaaaac aa
//
LOCUS       DAJOWB010000390          922 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJOWB010000390 DAJOWB010000000
VERSION     DAJOWB010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.400"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..607
                     /locus_tag="OHO85_005141"
     CDS             <1..607
                     /locus_tag="OHO85_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate"
                     /protein_id="HCQ2674331.1"
                     /translation="EFLKRTPRDRITLGTFSDVKEWLSSFEADRAGRKDTASTGPVNK
                     LAMPLVNLSRSPAFSIYEGELCRDNYDEGHVTNENDEIEALVSTIPFSLEYSLWIASD
                     EKESLGMVTTALAFWLRMYASLGQASFTHTANVGGYEIPVTCYIEGQKSIAFQDLTTG
                     TADNRLFAVGLNLTVVAELPILAYMQQTTGTITVKAKILEE"
     gene            609..>922
                     /locus_tag="OHO85_005142"
     CDS             609..>922
                     /locus_tag="OHO85_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCQ2674332.1"
                     /translation="MATKTTTAPETDSKRTQLFLQSVSIGQNEMPREMIVGCTYVEPG
                     ELSGPQLMLMVRDSTAYVVNKLGVKFGTILTVSLGDPEGHGGILFSEEFFVLKAPRKD
                     DTV"
BASE COUNT          241 a          219 c          236 g          226 t
ORIGIN      
        1 ggagttcctg aaacgcacac caagagatcg tataacgctc ggcactttta gtgatgtgaa
       61 ggagtggttg tcgtcatttg aagccgatcg cgccgggcgt aaagatacag cctctactgg
      121 cccggtaaat aagctggcaa tgccgcttgt gaatctgtct cgctctcccg ctttttcaat
      181 ttatgaaggt gaactgtgcc gcgataatta cgatgaaggg catgtcacaa atgaaaatga
      241 tgagattgaa gccctggtat cgactatccc tttctcactg gaatattcgc tatggatagc
      301 cagtgacgag aaggaatctc ttgggatggt tacaactgca ttagcattct ggctacgaat
      361 gtatgccagc ctcgggcagg catctttcac tcacactgcc aatgtcggcg gttatgagat
      421 accggttacc tgttacatag aagggcaaaa atcaatcgca tttcaggatc tgaccaccgg
      481 caccgccgac aacaggctgt tcgcggttgg attgaacctc acagtagtgg cggagcttcc
      541 tatcctggct tatatgcagc aaaccaccgg caccataacg gtaaaagcga aaattctgga
      601 ggaatgagat ggccacaaag accaccacag ccccggaaac tgattcaaaa cgcactcagc
      661 tattcctgca atctgtttca attgggcaga acgaaatgcc tcgcgaaatg atcgtaggat
      721 gtacctatgt cgaacctggg gagctatctg gtccccagct tatgctcatg gtcagggatt
      781 caacggctta cgtggtcaat aagctggggg tgaaatttgg gacaatactg acagtttcac
      841 ttggtgatcc ggaaggtcat ggcggcatcc tcttctcgga agagttcttt gttcttaaag
      901 cgccgcgcaa ggacgatact gt
//
LOCUS       DAJOWB010000391          907 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJOWB010000391 DAJOWB010000000
VERSION     DAJOWB010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.401"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            140..328
                     /locus_tag="OHO85_005143"
     CDS             140..328
                     /locus_tag="OHO85_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674333.1"
                     /translation="MKNRKAKILLVRRNAPGVWQWVRLSNRRMGLMKYYGMMDCGFCK
                     KPSAAQNRWKKHLRTKGE"
     gene            330..539
                     /locus_tag="OHO85_005144"
     CDS             330..539
                     /locus_tag="OHO85_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674334.1"
                     /translation="MAIAASYTMHLYCDCRQCTEGVYPVPDFGEYIGTSWAGCAKEAR
                     KDGWRISKDKTRAFAPGHKVLRINK"
     gene            536..>907
                     /locus_tag="OHO85_005145"
     CDS             536..>907
                     /locus_tag="OHO85_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674335.1"
                     /translation="MITITKERLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AEPVSQTYNLPELIEGMEVSIDVSTCDADLGNRYFGTVTEALELDTAKNGYILLVQDA
                     EPNFDVNGNSPVTPDSWISCSE"
BASE COUNT          260 a          200 c          251 g          196 t
ORIGIN      
        1 cgactcgttt ttgggcttct gcgctaattg caaagccgta tgtcctgatg gctgacaaca
       61 tcatcccggc accaaagcgc cgccataccg gtattgcagc ggcacgacga gcagcaaaga
      121 gacgcaggag agcaaagcga tgaaaaaccg taaggcaaag attctgttag ttcgtagaaa
      181 cgctcctggc gtctggcagt gggtgagact cagcaaccga cggatggggt tgatgaaata
      241 ttacgggatg atggattgtg gtttttgcaa aaagcccagc gcggcgcaaa accgctggaa
      301 aaaacacttg cgcactaaag gagagtgata tggctattgc cgcaagttac acaatgcatc
      361 tctattgtga ctgccgccag tgtacggaag gtgtatatcc agtgccagac ttcggtgagt
      421 atataggtac gtcatgggct ggctgtgcaa aagaggcgcg caaggatggc tggcgaataa
      481 gcaaagacaa aacgcgtgct tttgcgcccg ggcataaagt tttgaggatt aacaaatgat
      541 cactattacc aaagagcgac tgctgacaat caagcagtgg cgcgaaacat acggacctgg
      601 tagcaacgtt gtactgccag cagaagaagc ggaagaactg gcacgaattg cactggcatc
      661 gctggaagca gagcctgtaa gccaaactta caacttgcca gaattaatcg aaggcatgga
      721 agtttccatt gatgtaagca cttgtgatgc tgatttaggt aatcgctatt tcggcaccgt
      781 caccgaggcg ttagaacttg atactgccaa gaatggttac atcctcctgg ttcaggacgc
      841 agagccaaac ttcgatgtaa atggcaactc tccggtaact ccggatagtt ggataagctg
      901 tagtgag
//
LOCUS       DAJOWB010000392          905 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJOWB010000392 DAJOWB010000000
VERSION     DAJOWB010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.402"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            271..>905
                     /locus_tag="OHO85_005146"
     CDS             271..>905
                     /locus_tag="OHO85_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2674336.1"
                     /translation="MKKETNAFIMVDKRVAQFPKTLKPIDRLVYFTMLDGADYYESKF
                     GRYAPSIDYLMVMSGATRNTVIDSLKRLESHRLIEKTNVKGQQSIFKVFTLDERPEVL
                     SEPTLDERRRAKLSGQPESDSNPTKNWLPTQPEFGYLPNQNLVTCNTEYKDKNINTEE
                     ERLKGNFEEESLKKGASLCDSHKCDIKEDDFLSLDELNDFLSMNESDLDSEA"
BASE COUNT          292 a          152 c          178 g          283 t
ORIGIN      
        1 gtgaaataat tgttgcaatt tataattacg tctgttatag tttctatatg ggctgataaa
       61 aaagttcatg tcttgaagtg gtaacacttg gggaggtgtc agatggttgg cgtatattaa
      121 cctctcattc agggtatacc tcagcgtttt aatcatcgta ctgtttcagg catgggcttc
      181 tttatcagtc catcaatccc cagatatttt aatcacacag gctaatcggt tttatccggt
      241 tggccttttc acgtatgagg taatttatga atgaaaaaag aaactaatgc ttttattatg
      301 gtagataaac gagttgcaca attccctaaa acattaaagc caattgaccg cctggtatat
      361 ttcactatgc tggatggtgc tgattattac gaatctaagt tcgggagata tgcaccaagt
      421 attgactatt tgatggttat gtctggtgct acacgtaata cagttattga ttcacttaag
      481 agacttgagt cacacagact tatcgaaaag accaatgtaa agggtcagca aagtattttt
      541 aaagtattca cgttggatga gcgtccagag gtcttatcag aacctaccct tgatgagcgt
      601 cgtagagcta aattatcagg tcaaccagaa tctgacagta acccaaccaa aaattggtta
      661 cctacccaac cagaatttgg ttacctacct aaccagaatt tggttacatg taatactgaa
      721 tataaagaca aaaatataaa tactgaagaa gaaagactaa aagggaactt tgaagaagaa
      781 tctcttaaga agggagcttc gctctgtgat tcacataaat gtgatattaa agaagatgac
      841 tttctttctt tggatgaatt aaatgatttt ctctctatga atgaatctga ccttgatagt
      901 gaagc
//
LOCUS       DAJOWB010000393          894 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJOWB010000393 DAJOWB010000000
VERSION     DAJOWB010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.403"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..865
                     /gene="rdgC"
                     /locus_tag="OHO85_005147"
     CDS             <1..865
                     /gene="rdgC"
                     /locus_tag="OHO85_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCQ2674337.1"
                     /translation="READLTDLEDKKKMILFTPCGSQDMAKFGFVSPFGDNSEVIAMH
                     GNGFILVEAKRETKILPPPVIQRAIQEKIEKLEQEQARKLKKTEKDSLKDEVLHSLLP
                     RAFSKFSVIQAIYDGSTKRIYINASARQAEDMLALMRKSLGSLPVVPLSVENPIELTL
                     TDWVRDGSAPQGFQMGDAAELKAVLEDGGIARVKKQDLGSDEISTHLEAGKLVTKLAL
                     DWQNRIKFTLDHNFSLTSVKFADELLEQNSDIDSEDVAQRLDADFFLLTSEISCLVDA
                     LVNALGGEAKQ"
BASE COUNT          244 a          186 c          230 g          234 t
ORIGIN      
        1 tcgtgaggcg gatctgaccg acttagaaga taaaaagaaa atgatccttt tcacgccatg
       61 cggtagtcag gatatggcca agttcggttt tgtatcgcca tttggtgata attccgaagt
      121 tatcgctatg catggaaatg gttttatcct tgttgaagca aagcgcgaaa caaaaattct
      181 tcccccgccg gttatccagc gagctattca agaaaaaatt gaaaaacttg agcaagaaca
      241 agcgcgtaaa ctgaagaaaa cagagaagga ctccctgaaa gacgaagttc tacattctct
      301 tctgccacgg gctttttcaa agttttctgt tatccaggcg atctacgacg gttcaactaa
      361 acgtatctat atcaatgcca gcgcgcggca ggcagaggat atgctcgcgc ttatgcgtaa
      421 gtctctgggg tctcttcctg ttgttcccct gagtgttgaa aatcccattg aattaacgct
      481 gaccgactgg gtacgtgatg gtagtgctcc acagggattt caaatggggg atgcggcaga
      541 acttaaggca gtgcttgagg atggcggtat tgcccgagtg aaaaagcagg atttgggaag
      601 cgatgaaatt tccacacact tggaagcagg caagctcgtc actaagttgg cactcgactg
      661 gcagaaccgc attaaattta cactggacca taacttcagc cttaccagcg tcaaatttgc
      721 ggatgaattg cttgagcaga actctgatat tgatagtgaa gatgttgcgc agcgactgga
      781 cgcagatttc ttcctgttga ccagtgaaat ttcgtgcctg gttgatgctc tggtaaatgc
      841 ccttggtgga gaggctaagc agtgaaagag ctgtgctatg gatctgtttg cagt
//
LOCUS       DAJOWB010000394          879 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJOWB010000394 DAJOWB010000000
VERSION     DAJOWB010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.404"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            69..815
                     /gene="istB"
                     /locus_tag="OHO85_005148"
     CDS             69..815
                     /gene="istB"
                     /locus_tag="OHO85_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HCQ2674338.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
BASE COUNT          263 a          205 c          219 g          192 t
ORIGIN      
        1 ctgttatcca cgatggaaca cgaaaacgta cgcggcagtg agtattacca ctgatacggg
       61 atccaatgat gaatcatctt tacgaacaac tgaccgcact taaactcacc ggcttccgtg
      121 atgcgcttaa aaagcaactt gctcagccgg gcacatacca ggagctgggc ttcgaagaac
      181 gcctgtcatt actgacagca gaagaactaa cctgccgtga aaacaggaag gcagagcgtc
      241 tgatcaaaca tgcacggttc agacttaatg ctgagttatc aaagctggat tatcgtaaca
      301 atagagggct ggacagggcc ctcatccgtt cactcagtca gggaaactgg ttaaccctga
      361 aacaaaatat tttactgacc ggggccaccg gcagcggtaa aacgttcctg gcatgtgcac
      421 ttggtcataa tgcctgccga cagggataca aggtctacta ttatcgcctt aaagcgctga
      481 tggaacagtg ctatcagggg catgctgatg gaagatacag caaacttttg accaggctga
      541 ataatagcga tctgctgctt ctggatgact gggggctgga acctctctca tcagaacagc
      601 gtagcgacct gctggaaata gtggatctga tgtaccaacg aggctcaatc atcgtagtga
      661 gccagttgcc ggtggaaaac tggtacaaaa tgatcggaga ctccacacat gcggatgcca
      721 tcctagatcg actggttcat ggcagtatca agatcgaact taaaggagaa tcaatgcgga
      781 aaatacaatc tccgttgacc gaaggagatc agtgaaggta atttaaaaac ggttctgtga
      841 aagtgacacg aaccgatctc catcgatgtt actcaccga
//
LOCUS       DAJOWB010000395          875 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJOWB010000395 DAJOWB010000000
VERSION     DAJOWB010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.405"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            341..>875
                     /locus_tag="OHO85_005149"
     CDS             341..>875
                     /locus_tag="OHO85_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674339.1"
                     /translation="MIDVTNDKLLTQGTIFNCAYNSSYPDDETLGLIITARCDISNKD
                     KVSFYNYIPAIPFNIWKEKELLPVLKKKTYKDLRSKYLTLLREGGFSESNLKTYGYER
                     IIDIIKNKASLPKCKLKSLQTQHEKIECFEKKQPYAKLLSYFNKEIEKCLTDIIENKN
                     ADYFFIDYISGYGPAIVN"
BASE COUNT          321 a          134 c          155 g          265 t
ORIGIN      
        1 acaataaaac atctttcatc tattgagtac actggcgtaa aagtgggcta gactcaggtt
       61 gaagtaaatg taatcacacc atataatgca gtatttcgtt agtgtatgat gatgagtttt
      121 ttatgtgtca atagccggag agaaaaataa atccttgaaa tgccctttgt tgaacaatat
      181 gtatacaatg aaatgcatta ttttaatttt catgtagctc tgggagagag aacgcgtaac
      241 gttctttcct gttaatctga aagttgaatg aatataacca aaccactact cagagaagat
      301 aattaacaga aaaaatgggg gggggatagt gttccgcgac atgatagatg ttactaatga
      361 taagcttttg acccaaggga cgatctttaa ctgcgcctac aattcttcgt atcctgatga
      421 tgagacgtta gggttaatta ttactgcccg gtgtgatatt tccaacaaag acaaagtgag
      481 tttttataat tatataccag ccatcccttt taatatttgg aaagaaaaag aactattacc
      541 agtcttaaag aaaaaaacat ataaggatct aagaagtaag tatttaacct tgcttcgtga
      601 aggaggtttt tccgaaagca accttaaaac atatggatat gaaagaatta ttgatatcat
      661 caaaaataaa gcatctttgc caaaatgcaa actaaaatct ttacagaccc aacatgaaaa
      721 aatcgaatgt ttcgaaaaaa aacaaccata tgccaaacta cttagttatt tcaataaaga
      781 gatcgaaaag tgcttgactg atattattga aaataaaaat gcggattatt tttttattga
      841 ctatatttca ggttacggtc ctgcaattgt caatc
//
LOCUS       DAJOWB010000396          869 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJOWB010000396 DAJOWB010000000
VERSION     DAJOWB010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.406"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>869
                     /locus_tag="OHO85_005150"
     rRNA            <1..>869
                     /locus_tag="OHO85_005150"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          217 a          202 c          279 g          171 t
ORIGIN      
        1 ctaataccgc ataacgtcgc aagaccaaag agggggacct tcgggcctct tgccatcgga
       61 tgtgcccaga tgggattagc tagtaggtgg ggtaacggct cacctaggcg acgatcccta
      121 gctggtctga gaggatgacc agccacactg gaactgagac acggtccaga ctcctacggg
      181 aggcagcagt ggggaatatt gcacaatggg cgcaagcctg atgcagccat gccgcgtgta
      241 tgaagaaggc cttcgggttg taaagtactt tcagcgggga ggaagggagt aaagttaata
      301 cctttgctca ttgacgttac ccgcagaaga agcaccggct aactccgtgc cagcagccgc
      361 ggtaatacgg agggtgcaag cgttaatcgg aattactggg cgtaaagcgc acgcaggcgg
      421 tttgttaagt cagatgtgaa atccccgggc tcaacctggg aactgcatct gatactggca
      481 agcttgagtc tcgtagaggg gggtagaatt ccaggtgtag cggtgaaatg cgtagagatc
      541 tggaggaata ccggtggcga aggcggcccc ctggacgaag actgacgctc aggtgcgaaa
      601 gcgtggggag caaacaggat tagataccct ggtagtccac gccgtaaacg atgtcgactt
      661 ggaggttgtg cccttgaggc gtggcttccg gagctaacgc gttaagtcga ccgcctgggg
      721 agtacggccg caaggttaaa actcaaatga attgacgggg gcccgcacaa gcggtggagc
      781 atgtggttta attcgatgca acgcgaagaa ccttacctgg tcttgacatc cacagaactt
      841 tccagagatg gattggtgcc ttcgggaac
//
LOCUS       DAJOWB010000397          865 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJOWB010000397 DAJOWB010000000
VERSION     DAJOWB010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.407"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..732)
                     /locus_tag="OHO85_005151"
     CDS             complement(<1..732)
                     /locus_tag="OHO85_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="morphogenetic protein"
                     /protein_id="HCQ2674340.1"
                     /translation="MLLPLFPLPSRPTELIQFRQPNIADAMRFNSITPEEQEQQTTAY
                     LKALLAEPAKHDPLTWTAQDRITALWWIFTGSRETPVETFTYTCKHCGKEHYYDCDMN
                     ALAEDIQVLEVEPFIDDIEVSVEGVPYQWRIVPLDGWAMEMLEMRRAALPPEDDAEFK
                     EAIVDLRFWEFAYQCELYNDVSGTREDQAERRYETIKRMAIDTEFMKLAAHIRLAHEK
                     LEHGLPCYIDKGEMRLRLPPHKCPNQ"
BASE COUNT          229 a          211 c          200 g          225 t
ORIGIN      
        1 ctgatttggg catttatgcg gcgggagacg aagacgcatt tcacctttat cgatgtagca
       61 cggtaaacca tgttcgagct tttcatgagc cagtcggatg tgtgccgcca gcttcataaa
      121 ttcagtatca atggccatcc gtttaatcgt ttcataacga cgctcagcct gatcttcacg
      181 agtaccgcta acatcgttat aaagctcaca ctgataagcg aattcccaaa aacgcaaatc
      241 aacgatcgct tctttgaatt ccgcgtcgtc ttcaggtggc aatgctgcac ggcgcatctc
      301 cagcatttcc attgcccaac catcaagcgg cacgatacgc cattgataag gtactccctc
      361 tacagacacc tcaatatcgt caatgaaagg ttccacttcc aggacctgga tatcttcagc
      421 cagagcattc atatcgcaat cgtaataatg ctctttaccg caatgtttac aggtgtaggt
      481 gaatgtctcg accggtgttt cacgggagcc ggtaaatatc caccataacg cggtaatccg
      541 gtcctgcgcc gtccatgtca ggggatcatg tttcgcgggt tcagcaagca aggcttttaa
      601 atacgccgtt gtctgttgtt cttgttcctc cggtgttatc gagttgaaac gcatcgcatc
      661 agcaatattt ggctgacgga actggatcaa ttcagttggc cgcgatggta gcgggaaaag
      721 aggtaaaagc atccttgctc cttaattcaa agagaaaagc taaagcccag aagggaagcc
      781 aaagaacttg aggattggtt aaacgtgctg tgcaatgcga aggtcattgg gaatgactta
      841 aattcagtaa cctgatctcg cgcat
//
LOCUS       DAJOWB010000398          847 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJOWB010000398 DAJOWB010000000
VERSION     DAJOWB010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.408"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>847
                     /gene="ag43"
                     /locus_tag="OHO85_005152"
     CDS             <1..>847
                     /gene="ag43"
                     /locus_tag="OHO85_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCQ2674341.1"
                     /translation="GTTNGMTISTGLEYGPDNEANTGGQWVQDGGTANKTTVTSGGLQ
                     RVNPGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHEGAIATGTVINDKGWQVVKP
                     GTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINKNGRQIVRAEGTANTTVVYA
                     GGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQIVKNGGVAGNTTVNQKGRL
                     QVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVVEGKADNVVLENGGRLDVLT
                     GHTAT"
BASE COUNT          221 a          200 c          281 g          145 t
ORIGIN      
        1 tcggtacgac caacggaatg accatcagta ccgggctgga gtatgggccg gataacgagg
       61 ccaataccgg cgggcaatgg gtacaggatg gcggaacagc caacaaaacg actgtcacca
      121 gtggtggtct tcagagagtg aaccccggtg gaagtgtctc agacacggtt atcagtgccg
      181 gaggcggaca gagccttcag ggacgggcag tgaacaccac gctgaatggt ggcgaacagt
      241 ggatgcatga gggggcgata gccacaggaa ccgtcattaa tgataagggc tggcaggtcg
      301 tcaagcccgg tacagtggca acggataccg ttgttaatac cggggcggaa gggggaccgg
      361 atgcagaaaa cggtgatacc gggcagtttg ttcgcgggga tgccgtacgc acaaccatca
      421 ataaaaacgg tcgccagatt gtgagagctg aaggaacggc aaataccact gtggtttatg
      481 ccggcggcga ccagactgta catggtcacg cactggatac cacgctgaat gggggatacc
      541 agtatgtgca caacggcggt acagcgtctg acactgttgt gaacagtgac ggctggcaga
      601 ttgtcaaaaa cgggggtgtg gccgggaata ccaccgttaa tcagaagggc agactgcagg
      661 tggacgccgg tggtacagcc acgaatgtca ccctgaagca gggcggcgca ctggttacca
      721 gtacggctgc aaccgttacc ggcataaacc gcctgggagc attctctgtt gtggagggta
      781 aagctgataa tgtcgtactg gaaaatggcg gacgcctgga tgtgctgacc ggacacacag
      841 ccactaa
//
LOCUS       DAJOWB010000399          845 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJOWB010000399 DAJOWB010000000
VERSION     DAJOWB010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.409"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>845
                     /locus_tag="OHO85_005153"
     CDS             <1..>845
                     /locus_tag="OHO85_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCQ2674342.1"
                     /translation="GAVLDKLLDNCNNINLLLLVRAPTPQAGLERIKENMRKFNVCEE
                     RLHALTNDNILPGDLNNPEAFLMDPRLDEVTHVINCAAIASFGNNPFIWNVNVTGTLA
                     FARRMAKVAGLKRFLHVGTAMSCTPHTGSLVKEESASSETGEHLVEYTHSKATIEYLM
                     RKQCPDLPLLVARPSIIVGHSRLGCLPSTSIFWVFRMGLMLQKFMCSMDDKIDVIPVD
                     YCADALLMLLESSLINGEIVHISAGKESSVTFSAIDEAVARALNCDPVGDRYTKVSYD
                     ILAMSR"
BASE COUNT          240 a          142 c          195 g          268 t
ORIGIN      
        1 ggcgcggtcc tggataagct gctggataac tgtaataata taaatttgct acttttagta
       61 cgagcaccta ctccacaagc gggactggaa agaattaaag aaaatatgcg taaatttaat
      121 gtttgtgagg aaaggttgca tgcattaact aatgataaca tcttgcctgg ggatctaaat
      181 aatccggaag cctttctcat ggatcctcgt cttgatgaag tcactcatgt tataaactgt
      241 gcggctatag cttcttttgg taataatcct tttatatgga atgtgaatgt tacaggtaca
      301 cttgcttttg caagaagaat ggcaaaagtg gcaggactga aacgcttcct tcatgttggt
      361 actgctatgt cttgtacacc tcatacgggg tcgctagtta aggaagagtc tgcttcatca
      421 gaaacaggtg aacatttagt ggagtatacg cattcaaaag caacaataga atatctgatg
      481 cgtaagcagt gtcctgattt acctttgttg gttgcccgac catcaattat tgttggccac
      541 agtcgtttag ggtgcttacc ttcaaccagt attttctggg tattcagaat ggggttaatg
      601 ttgcaaaaat ttatgtgctc tatggatgat aaaatagatg ttattcctgt agattattgt
      661 gctgatgcat tgctaatgtt gcttgaaagc tcgttaatta atggtgagat tgttcatata
      721 tcagcaggta aagaaagtag tgtgacgttc tctgctattg acgaagctgt agcccgtgct
      781 ttgaactgtg atcctgttgg agacagatat actaaagtca gttatgacat actggcaatg
      841 agccg
//
LOCUS       DAJOWB010000400          844 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJOWB010000400 DAJOWB010000000
VERSION     DAJOWB010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.410"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            724..>844
                     /gene="iss"
                     /locus_tag="OHO85_005154"
     CDS             724..>844
                     /gene="iss"
                     /locus_tag="OHO85_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HCQ2674343.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHH"
BASE COUNT          261 a          166 c          168 g          249 t
ORIGIN      
        1 cctaagacat caatcatctg ctctccaatg actagtctaa aaactagtat taagactatc
       61 acttaaataa gtgatactgg ttgtctggag attcaggggg ccagtctatt ctccttcacg
      121 gtaatacgca gttatagttg cagagcaatg taatggatct tttatattaa gaaattgtgt
      181 gagtggagag gtgcacgaat cctagaggca gaatgtttct ccacatgctt gtggagaaac
      241 cgccgcattt tgcccgttct gtacgatact ccgtagccag agataagata ttctgtcgct
      301 gtatgctaca tacattacac tatacgaaat caggtctgtt caatcagtaa ggaaataaac
      361 ggacaaacgt tatgtaaccc agacagagat atattgtctg ggaaaatagt ggcttttgtg
      421 gcgaaaacta gtaaaacagc aacccgaacc acttgatgtg catcgttttt gattattccc
      481 gtatactctt gcagaaggag ttctccgtcg ggctactgtc atggttaatg cggggaatat
      541 ggcgacaata caacacacct aaaagagtaa tggacagatg aagcggttta ttcatttccc
      601 atgattctga gtacctacca agtctgagta accactttta tacttttaat tttcgttcat
      661 ttagctatcg tttaattatt atcacatagg attctgccgt ttttaacaat gcaggataat
      721 aagatgaaaa aaatgttatt ttctgccgct ctggcaatgc ttattacagg atgtgctcaa
      781 caaacgttta ctgttggaaa caaaccgaca gcagtaacac caaaggaaac catcactcat
      841 catt
//
LOCUS       DAJOWB010000401          841 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJOWB010000401 DAJOWB010000000
VERSION     DAJOWB010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.411"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>841
                     /locus_tag="OHO85_005155"
     CDS             <1..>841
                     /locus_tag="OHO85_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="head processing protein"
                     /protein_id="HCQ2674344.1"
                     /translation="KETQEGLRLGELFGYYGHGRRQLTGKLEVPETSVIMVEGRPVVI
                     DNVPACRTVAISVDDNGIVTHTQEILNTEPGKIVAAMIESRAGGWSWATGGRESGKIA
                     VTTSFHGVDYVTTPNYISLDHPASAGMFESADSKSLLAESLAAHGYSDESVQAVISHY
                     GKMAELEMMVEATERTAELETALLESQGRHLEAMAKIADAEARIALLEETAGIRDDVL
                     AAMQDELDNLPIFVSAAQKDAFRLKEPGDAKIVATLFESLIKVGARNLPVTRKIKEVP
                     QAA"
BASE COUNT          219 a          218 c          238 g          166 t
ORIGIN      
        1 gcaaggaaac tcaggaaggt ttacgccttg gtgagctttt cggctattac ggtcacggtc
       61 gccgacagct gactggcaaa ctggaagtac cagaaaccag cgtgatcatg gtggaaggtc
      121 gcccggtcgt aatcgacaat gttccggcgt gtcgcacagt ggctatatct gttgacgaca
      181 acggcatcgt tacccataca caggaaattc ttaacacaga gccgggtaaa attgtcgccg
      241 cgatgatcga aagccgagct ggtggctgga gctgggccac tggcgggcgc gagtccggga
      301 aaatcgctgt aaccaccagc ttccatggtg tggattatgt gacaacgccg aactatatca
      361 gtctggatca tcctgccagc gccggaatgt ttgaaagcgc ggattctaaa tctctactgg
      421 cagagtccct ggcggcgcat gggtactccg acgagtcagt gcaggcagtt atatcccatt
      481 acggcaaaat ggctgaactg gaaatgatgg tggaggcgac agagcgtacg gcagaactgg
      541 aaaccgcact actcgaaagc cagggccgcc acctcgaagc aatggccaag atcgcagatg
      601 ctgaagcgcg aatcgctttg ctggaggaaa cagcgggtat ccgcgacgat gtgctggcag
      661 caatgcaaga cgaactggat aacctcccga tcttcgtctc cgccgcccaa aaagacgcat
      721 tccgcctcaa agaacctggt gatgcaaaaa tcgttgccac acttttcgaa tctctgatca
      781 aagttggcgc acgcaacttg cctgtcacca ggaaaattaa ggaggttccg caagcggcta
      841 a
//
LOCUS       DAJOWB010000402          839 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJOWB010000402 DAJOWB010000000
VERSION     DAJOWB010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.412"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            167..>839
                     /locus_tag="OHO85_005156"
     CDS             167..>839
                     /locus_tag="OHO85_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001632353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674345.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKKPATMALHNLA
                     NDILREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSSGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGESSSVRKKRLRLA
                     RLLLSLIDDAGLNKLDSLANPRIRTNYRECLDAIRQVTLQQEYIRGRSLSEIIHFRPG
                     MSERSQ"
BASE COUNT          225 a          182 c          191 g          241 t
ORIGIN      
        1 tattctctca ctttaatgtt gtcatgatat aaataaaaac aattcatttg aaaggatatc
       61 agaagaagat atcggggcgc acagttcatc tctgcctttg tttcatgggg tgttctttgt
      121 atattttact tactgtttaa gcatgtacat aaggagtgct ttcattatgc caatgatact
      181 tgtaaagaag aatccccggg gcaaagtgat tcgggagctt agcagtgagg aagagactgc
      241 agtaaagacg gtctgtggac tgaaaaagcc agcaaccatg gcactacata acctggccaa
      301 tgatattctc cgggaaatgc gtgagtatga tgcatggtta cagtgtgact gtattccggg
      361 agattcgccg gccatgaatt ttgctgctct taaaaacaat acgggtactc tttatctgtc
      421 cagttttaat catgagcatg cacctgaatg ccccatgtat cgtcagttat ccggaaatga
      481 agaggaaaca tcttccggag caagccggca tcccgttagc acccgtataa attacagaaa
      541 ctttttacca cctgatgaca gtaattctgt tatcagactg caggcccgct ctgcttacca
      601 taatggtgag agctcctccg ttcgcaagaa acgtctgcgc cttgcccgtt tactcctgtc
      661 tcttattgac gatgctgggc tgaataaact ggattccctg gctaatcccc gtatccggac
      721 taattatcgt gagtgtcttg atgccatacg gcaggttaca ctgcagcagg agtatattcg
      781 gggaagatcc ttatctgaaa ttatccattt ccggcctgga atgtctgaaa ggtctcagg
//
LOCUS       DAJOWB010000403          832 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJOWB010000403 DAJOWB010000000
VERSION     DAJOWB010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.413"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>832
                     /locus_tag="OHO85_005157"
     CDS             <1..>832
                     /locus_tag="OHO85_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HCQ2674346.1"
                     /translation="VVIDFTPTVCSAFSYAFISVLKKRYSFDKIALAIDNRPSSYCIA
                     KYCAAALSDLGIETVYYGVIPTPALAYQAMKDNIPGIMVTGSHIPYDRNGLKFYTPEG
                     EITKADEQEILLEDVLCSQPKAYPELQVNKKAAESYCSRYISLFDNTVFKGARIGIYE
                     HSSAGRDFYHYIFKALGADVISLGRCDDFIPIDTEAVAEDDRAKAKEWARKYNLDAIF
                     STDGDGDRPLLADENGEWLRGDVLGFLAAEALSIEALAIPINCNTSIFESKRFDEVLL
                     T"
BASE COUNT          249 a          114 c          183 g          286 t
ORIGIN      
        1 gggtagttat tgactttact ccaactgtct gctctgcttt ttcatacgcc tttatatctg
       61 ttttgaaaaa aagatatagt ttcgataaaa tcgcattagc tattgataat cgtcctagta
      121 gttattgcat agcaaaatat tgtgctgctg cgttatcaga tttaggaatt gaaactgttt
      181 attatggagt gatacctact cctgctcttg cttatcaggc aatgaaagat aatattcctg
      241 gtattatggt tacgggcagt catataccat atgatagaaa tggtttaaaa ttttatactc
      301 ctgaaggaga aataactaaa gctgatgagc aggagatttt attagaagat gttctttgtt
      361 cacaacctaa agcatatcct gagcttcagg ttaataaaaa ggctgcggaa agttattgct
      421 caagatatat ttcattattt gataatactg tatttaaagg agcgcggatt ggtatttatg
      481 aacactcatc tgcgggaaga gatttttatc actatatttt taaagcgctt ggtgcagatg
      541 tgatttctct cggacgttgt gatgatttca taccaattga tacggaagct gttgcagagg
      601 atgatagagc taaagcaaag gaatgggcaa gaaaatataa tcttgatgct attttttcta
      661 ctgatggcga tggagataga ccattattag ctgatgaaaa tggggagtgg ttgagaggcg
      721 atgtattggg ctttttggct gctgaagctt taagcattga agccttggca atacctatta
      781 attgtaatac atctattttt gagagtaaga ggtttgatga ggttttattg ac
//
LOCUS       DAJOWB010000404          825 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJOWB010000404 DAJOWB010000000
VERSION     DAJOWB010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.414"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(31..423)
                     /locus_tag="OHO85_005158"
     CDS             complement(31..423)
                     /locus_tag="OHO85_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674347.1"
                     /translation="MSDKVTVKQTINKATSIYKIEQITVGKPGSEQYRRAFELADQLG
                     LKHPDCIEHVFPTYADEQCTHVLTEEDFFSTEEREGVDRCIGVICSSVSYELFPNVHE
                     DGGVGYQFLYEGDELKCYEHGLLIESVE"
     gene            complement(601..>825)
                     /locus_tag="OHO85_005159"
     CDS             complement(601..>825)
                     /locus_tag="OHO85_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ppfA"
                     /protein_id="HCQ2674348.1"
                     /translation="QTIETMNKAEQHFHSQEVKNESEQAKYADRQMERKAEVQKQLVA
                     AGNVRQRIPADTQRLLRESISEFNADADKG"
BASE COUNT          212 a          195 c          166 g          252 t
ORIGIN      
        1 gccaacaaag ccggttggaa ggaagtcata ttattctacg ctttcgataa gaagaccatg
       61 ttcataacat ttaagctcat cgccttcata caggaattgg tatccaacac caccatcttc
      121 atggacatta gggaataact cataactcac tgaagagcaa atcacaccaa tgcagcgatc
      181 aactccttct cgttcttcag tgctgaaaaa atcctcttcg gtaagaacat gagtacattg
      241 ctcatcagca taggtcggaa atacatgctc aatgcaatcc gggtgtttta aaccaagctg
      301 atcggcaagc tcgaaagcac gacggtattg ttcagatcct ggcttgccaa cagtgatttg
      361 ctcaattttg tagattgaag tcgctttgtt gatagtttgc tttactgtta ctttatcaga
      421 cataaaaatc ccttttagtt accgctaata gctcggttgt aatcattaac gttgcgattc
      481 ttcctgttaa tccccatcag catcgtttct gtatcgagga tatacgctgg cagatcatca
      541 aaatattcac tgctaaactc tggcatcctg cacataaatg cacttttggg ggcagggtgg
      601 ttaacctttg tcggcgtcgg cgttaaattc gctgatcgac tcccggagca accgctgagt
      661 gtcagcagga atacgctggc gaacattacc cgccgcaacc agttgtttct gaacttcagc
      721 ttttcgttcc atttgcctgt cagcatactt ggcttgttct gattcatttt tcacttcctg
      781 gctgtgaaaa tgttgctctg ctttgttcat cgtctcaatg gtctg
//
LOCUS       DAJOWB010000405          825 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJOWB010000405 DAJOWB010000000
VERSION     DAJOWB010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.415"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            18..>825
                     /locus_tag="OHO85_005160"
     CDS             18..>825
                     /locus_tag="OHO85_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCQ2674349.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHP"
BASE COUNT          261 a          182 c          148 g          234 t
ORIGIN      
        1 tataaccgga gtagtgtatg gccaatctct acgacttgaa aaagttcgac ttaaaccttc
       61 ttgtcatatt tgagtgtatt taccagcatt taagcattag caaagctgct gaatcactat
      121 atatcacccc atcagccgtg agccagtcgt tacaacgctt acgtgcgcaa tttaatgacc
      181 ctttattcat tcgctcagga aaaggtatcg cgccgacgac aacagggttg aatctgcatc
      241 accatcttga gaaaaacctc aaaggccttg aacaaacaat taatatagtc aataagtccg
      301 aactaaagaa aaactttatt atctacggcc ctcagctcat ttcttgctct aataatagta
      361 tgctgatccg ctgcctacgg caagatgcct ctgtcgagat tgagtgccat gatatcctca
      421 tgtcagcaga gaatgctgaa gaactgttgg ttcaccgcaa agccgatctg gtgatcaccc
      481 aaatgccagt gatcagccgt tccgtcattt gcatgccatt gcatacaatt cgcaatactc
      541 taatttgtag taataagcat ccgcgaatta ctgacaatag cacttatgaa caaattatgg
      601 cagaagagtt cacccaatta atatccaaat cagcaggagt tgatgatatt caaatggaaa
      661 tagacgagag atttatgaat cgcaaaatct catttcgtgg ttcctctttg ctgacaatca
      721 ttaatagcat tgcagttaca gatttactgg gaatagtccc ctatgaactt tataattcct
      781 accgtgactt tcttaattta aaagagataa aactagaaca tccgt
//
LOCUS       DAJOWB010000406          822 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJOWB010000406 DAJOWB010000000
VERSION     DAJOWB010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.416"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>822
                     /locus_tag="OHO85_005161"
     CDS             <1..>822
                     /locus_tag="OHO85_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCQ2674350.1"
                     /translation="DRSWRVGQYYDCAGDIVRIKSLDFDSQRADVEIIFTFKGTKSGN
                     WDVKTLDKQVDVTPDEDAVMQKISGGVSIAGINDIISCDDFYRFQQRGMIKITDSYGV
                     QTTESGYSIDFVGTYTDPLKHAVYPDRRDGALKSSIAKWVLGMMSEGNNRQIRSAETF
                     LVELFGSNYGDVIASYGDTLSPEAIQEKIADAIARMPEKTSQGATRNGDSELEVTNAI
                     FGTNEFRASDYEITTAQFGTIGIYSNKAEIKQAMDAASARIAAEREANLNHAVAAL"
BASE COUNT          220 a          197 c          221 g          184 t
ORIGIN      
        1 gatcgctcat ggcgtgtagg ccaatactat gattgtgccg gtgatatcgt tcgcattaag
       61 tcgctggact tcgacagcca gcgcgcagac gtggagatca tctttacctt caaaggcacc
      121 aaatcgggta actgggatgt gaagacgctg gataaacagg ttgatgtaac tcccgatgaa
      181 gatgctgtta tgcagaaaat cagtggtggc gtctccatcg ccgggattaa cgacatcatt
      241 tcctgtgacg atttctaccg tttccagcag cgcggcatga tcaaaatcac tgactcatac
      301 ggcgttcaga ctacagagtc aggctatagc atcgattttg ttggtaccta tacggaccca
      361 ctgaagcatg cggtttaccc ggatcgccgt gacggcgcgc tgaagtcgtc aattgcaaaa
      421 tgggtgcttg gtatgatgtc ggaagggaat aaccgccaga tccgttcggc agaaacattc
      481 ctggttgaac tgtttggctc caattatggc gatgtaatcg cgtcatacgg agatacgcta
      541 tcccctgaag caattcagga gaaaatagcg gatgcgatcg ccagaatgcc ggaaaaaaca
      601 agccaggggg ctactcgtaa cggggattct gaacttgaag tcaccaatgc cattttcggt
      661 accaatgagt tccgggcgtc agattatgag atcaccacag cacagtttgg caccattggc
      721 atttacagca ataaagccga gatcaagcag gcaatggacg cagcaagcgc gcgcatcgca
      781 gcagaacggg aagccaatct gaatcatgca gtcgccgcgc tg
//
LOCUS       DAJOWB010000407          815 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJOWB010000407 DAJOWB010000000
VERSION     DAJOWB010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.417"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..815
                     /locus_tag="OHO85_005162"
                     /pseudo
     CDS             1..815
                     /locus_tag="OHO85_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          205 a          224 c          225 g          161 t
ORIGIN      
        1 gacaaaaaca gtatcaacca gtaaaaaacc ccgtaaacag cattcgcctg aatttcgcag
       61 tgaagccctg aagcttgctg aacgcatcgg tgttactgcc gcagcccgtg aactcagcct
      121 gtatgaatca caactctaca actggcgcag taaacagcaa aatcagcaga cgtcttctga
      181 acgtgaactg gagatgtcta ccgagattgc acgtctcaaa cgccagctgg cagaacggga
      241 tgaagagctg gctatcctcc aaaaggccgc gacatacttc gcgaagcgcc tgaaatgaag
      301 tatgtcttta ttgaaaaaca tcaggctgag ttcagcatca aagcaatgtg ccgcgtgctc
      361 cgggtggccc gcagcggctg gtatacgtgg tgtcagcggc ggacaaggat aagcacgcgt
      421 cagcagttcc gccaacactg cgacagcgtt gtcctcgcgg cttttacccg gtcaaaacag
      481 cgttacggtg ccccacgcct gacggatgaa ctgcgtgctc agggttaccc ctttaacgta
      541 aaaaccgtgg cggcaagcct gcgccgtcag ggactgaggg caaaggcctc ccggaagttc
      601 agcccggtca gctaccgcgc acacggcctg cctgtgtcag aaaatctgtt ggagcaggat
      661 ttttacgcca gtggcccgaa ccagaagtgg gcaggagaca tcacgtactt acgtacagat
      721 gaaggctggc tgtatctggc agtggtcatt gacctgtggt cacgtgccgt tattggctgg
      781 tcaatgtcgc cacgcatgac ggcgcaactg gcctg
//
LOCUS       DAJOWB010000408          811 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJOWB010000408 DAJOWB010000000
VERSION     DAJOWB010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.418"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..307)
                     /locus_tag="OHO85_005163"
     CDS             complement(<1..307)
                     /locus_tag="OHO85_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCQ2674351.1"
                     /translation="MENNLENLTIGVFAKAAGVNVETIRFYQRKGLLREPDKPYGSIR
                     RYGEADVVRVKFVKSAQRLGFSLDEIAELLRLDDGTHCEEASSLAEHKLKDVREKMAD
                     "
     gene            379..729
                     /locus_tag="OHO85_005164"
     CDS             379..729
                     /locus_tag="OHO85_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transport protein MerT"
                     /protein_id="HCQ2674352.1"
                     /translation="MSEPQNGRGALFAGGLAAILASTCCLGPLVLVALGFSGAWIGNL
                     TVLEPYRPLFIGAALVALFFAWKRIYRPVQACKPGEVCAIPQVRATYKLIFWIVAVLV
                     LVALGFPYVVPFFY"
     gene            743..>811
                     /locus_tag="OHO85_005165"
     CDS             743..>811
                     /locus_tag="OHO85_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transport protein periplasmic
                     component"
                     /protein_id="HCQ2674353.1"
                     /translation="MKKLFASLALAAAVAPVWAATQT"
BASE COUNT          136 a          259 c          223 g          193 t
ORIGIN      
        1 agtcggccat cttctcgcgc acgtccttga gcttgtgttc ggccaggctg ctggcctcct
       61 cgcagtgggt gccatcgtcg agccgcaaca gctcggcaat ctcgtccaga ctgaacccca
      121 gccgctgtgc cgatttcacg aatttcaccc gaaccacgtc cgcctcccca tagcggcgga
      181 tgctgccgta aggcttgtcc ggttcccgca acaggccctt gcgctgatag aagcggattg
      241 tctccacgtt gaccccggcc gccttggcaa aaacgccaat ggtcaggttt tccaaattat
      301 tttccatatc gcttgactcc gtacatgagt acggaagtaa ggttacgcta tccaatccaa
      361 attcaaaagg gccaacgtat gtctgaacca caaaacgggc gcggtgcgct cttcgccggc
      421 gggctggccg ccattcttgc atcgacctgc tgcctggggc cgctagtact ggtcgccctg
      481 ggcttctccg gtgcttggat cggcaacctg acggtgctgg aaccctatcg accgttgttc
      541 atcggcgcgg cgctagtggc gctgttcttc gcctggaagc ggatttaccg gcccgtgcag
      601 gcatgcaagc caggtgaggt ctgcgcgatt ccgcaggtgc gcgccaccta caagctgatt
      661 ttctggatcg tggccgtgct ggtcctggtc gcgcttggat ttccctatgt cgttccattt
      721 ttctattaac caggagttca tcatgaagaa actgtttgcc tcccttgccc tcgccgccgc
      781 tgttgccccg gtgtgggccg ctacccagac c
//
LOCUS       DAJOWB010000409          801 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJOWB010000409 DAJOWB010000000
VERSION     DAJOWB010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.419"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            62..619
                     /locus_tag="OHO85_005166"
     CDS             62..619
                     /locus_tag="OHO85_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCQ2674354.1"
                     /translation="MKGKTAAGGGAICAIAVMITIVMGNGNVRTNQAGLELIGNAEGC
                     RRDPYMCPAGVWTDGIGNTHGVTPGVRKTDQQIAADWEKNILIAERCINQHFRGKDMP
                     DNAFSAMTSAAFNMGCNSLRTYYSKARGMRVETSIHKWAQKGEWVNMCNHLPDFVNSN
                     GVPLRGLKIRREKERQLCLTGLVNE"
BASE COUNT          204 a          175 c          240 g          182 t
ORIGIN      
        1 cggtattggc tgtgaatggg tgtttgtcct ggagcgtaaa aaattcgctt atgaggtctt
       61 tatgaaggga aaaacagccg caggaggcgg tgcaatttgc gctatcgcgg tgatgattac
      121 catcgtgatg ggtaatggca atgtgcgaac caaccaggcg gggcttgagc tgattggtaa
      181 cgctgaaggt tgccgacgtg atccatacat gtgcccggcg ggggtatgga ctgacgggat
      241 cggtaataca cacggggtaa cgccgggtgt gcgaaaaacc gaccagcaaa tcgccgctga
      301 ttgggaaaag aatatcctga tcgctgaacg ctgtattaat cagcacttcc ggggcaaaga
      361 catgcccgat aatgccttca gtgcaatgac aagcgcggca ttcaatatgg gatgcaatag
      421 cttacggacc tactacagca aagcgcgagg catgcgagta gaaacgtcca tccacaagtg
      481 ggcgcagaaa ggggaatggg tgaatatgtg taaccatctc cctgatttcg tgaacagtaa
      541 cggcgtgccc ctgcgaggtt taaagattcg ccgtgaaaaa gaacgccagc tttgcctgac
      601 ggggcttgtc aatgaataaa ctccggcagc tccgccgact ttcgacaatg aagttatcgc
      661 tggcggcgat agtttttgac tcgattttca tggcggtata tgtgctcaat gagacgtggc
      721 cactggaacc gctattgtat gccgggcttc ggctgtgcct gacatttttg agcatggctg
      781 cgagattgat gcagcagaaa g
//
LOCUS       DAJOWB010000410          796 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJOWB010000410 DAJOWB010000000
VERSION     DAJOWB010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.420"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>796
                     /locus_tag="OHO85_005167"
     CDS             <1..>796
                     /locus_tag="OHO85_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HCQ2674355.1"
                     /translation="FYQPWVNALPPMPRHANPEQLEKTVRYLTQTVHPRSADNIDNLN
                     RSAEYIKEVFVSSGARITSQDVPITGGPYKNIVADYGPADGPLIIIGAHYDSASSYEN
                     DELTYTPGADDNASGVAGLLELARLLHQQVPKTGVQLVAYASEEPPFFRSDEMGSAVH
                     AASLERPVKLMIALEMIGYYDSAPGSQDYPYPAMSWLYPDRGDFIAVVGRMQDINAVR
                     QVKATLLSSRYLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAVMITDT"
BASE COUNT          203 a          195 c          188 g          210 t
ORIGIN      
        1 ttttttacca accctgggtc aatgcattac cgccaatgcc ccgacacgca aatcctgagc
       61 aattagaaaa aacagttcgt tatcttacac aaactgtgca tccacgtagt gccgacaata
      121 ttgataatct gaataggtcc gcagagtata taaaagaggt cttcgtcagt agcggtgcca
      181 gaattacctc gcaggacgtc cccattacgg gtggccccta caaaaacatt gttgctgatt
      241 atggtcctgc cgatggaccg ctgattatta ttggtgcgca ctatgactct gccagcagtt
      301 atgaaaacga tgaattgacc tatacacctg gcgcggatga taacgccagc ggcgtggcag
      361 gattactcga actggcacgt ttgttacatc agcaagtacc gaaaacaggc gtgcagctgg
      421 tcgcctatgc gtcggaagaa ccgcccttct ttcgtagcga tgaaatgggg agcgcggtac
      481 atgcagcttc gcttgagcgt ccagtaaaat taatgatagc actggagatg attggctatt
      541 acgactctgc gcctggtagc caggattacc cttacccggc aatgtcctgg ctttatcccg
      601 atcggggaga ctttattgcc gtggtcggca gaatgcagga tatcaacgcc gttcgtcagg
      661 taaaagcgac attgttatca tcccggtatt tatctgttta ttctatgaat gccccagggt
      721 ttattcccgg tattgatttc tctgatcacc ttaattattg gcaacacgat attcccgccg
      781 taatgattac tgacac
//
LOCUS       DAJOWB010000411          792 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJOWB010000411 DAJOWB010000000
VERSION     DAJOWB010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.421"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            175..250
                     /locus_tag="OHO85_005168"
     tRNA            175..250
                     /locus_tag="OHO85_005168"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:208..210,aa:Ile,seq:cat)
BASE COUNT          231 a          154 c          178 g          229 t
ORIGIN      
        1 taacatttgc ttgttgactg cgagtatcga ctttgcgaat ggaagcggtg tcgggtttta
       61 tgtcgcgtgt aatgacgagc aaggatgaat gagaacgcat aaaaatctgt tatttacatc
      121 atatgatcat catgtttatt gcgtggttta taagtattgt tattgcacac tcaaggccct
      181 ttagctcagt ggttagagca ggcgactcat aatcgcttgg tcgctggttc aagtccagca
      241 agggccacca aatcattatt tacgtatcag gaagagttaa gggtacaagg ggtactcgat
      301 ggcggtctat atttcatgac taacgccatt tcaaggaaga agtgcggtgg gttgtttcga
      361 atgagggctg gtgttttggt acgtggcacg ccactgcaat gtatttatcc atcagtactc
      421 attcaaaagt acctggaaaa aatgcccgct atgccaagcg ggcaaattgc ttacatgtgg
      481 aattaagtaa ttaacgataa agccagagta ttatcattta ttatacccct taatttaatc
      541 aggttaatta ctcaaatcac tgaactattc tctgttcgct cactgatgat gagtgagtac
      601 cacaaccaat gtatgtagaa caatgccgta gagtaaggat gaaatgtctc tgccacgcct
      661 taaacatcta ctactgacca gaacacccgc cgcaggcaga tgagttttgt gagcgttaca
      721 ggagcgtggc tatgctcatt aaccaacgct attgagatat aaatttctaa cactcgaaaa
      781 acgaatttca ct
//
LOCUS       DAJOWB010000412          787 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJOWB010000412 DAJOWB010000000
VERSION     DAJOWB010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.422"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            30..746
                     /locus_tag="OHO85_005169"
     CDS             30..746
                     /locus_tag="OHO85_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCQ2674356.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPVPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          259 a          134 c          168 g          226 t
ORIGIN      
        1 gtaaataaat tttcgaggag atcattccag tgggacgtaa atgggccaat attgttgcta
       61 aaaaaacggc taaagacggt gcaacgtcta aaatttatgc aaaattcggt gtagaaatct
      121 atgctgctgc taaacaaggt gaacccgttc cagaattaaa cacatcttta aaattcgtta
      181 ttgaacgtgc aaagcaggca caagttccaa aacacgttat tgataaagca attgataaag
      241 ccaaaggcgg cggagatgaa acgttcgtgc agggacgtta tgaaggcttt ggtcctaatg
      301 gctcaatgat tatcgccgag acattgactt caaatgttaa ccgtacgatt gctaacgttc
      361 gcacaatttt caataaaaaa ggcggcaata tcggagcggc aggttctgtc agctatatgt
      421 ttgacaatac gggtgtgatt gtatttaaag gaacagaccc tgaccatatt tttgaaattt
      481 tacttgaagc tgaagttgat gttcgtgatg tgactgaaga agaaggtaac attgttattt
      541 atactgaacc tactgacctt cataaaggaa tcgcggctct aaaagcagct ggaatcactg
      601 agttctcaac aacagaatta gaaatgattg ctcaatctga agttgagctt tccccagaag
      661 atttagaaat ctttgaaggg cttgttgatg cccttgaaga tgacgacgat gttcaaaaag
      721 tttatcataa cgtcgcaaat ctctaattat attttaaaga aatctgtctt tatggcagat
      781 ttcttta
//
LOCUS       DAJOWB010000413          784 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJOWB010000413 DAJOWB010000000
VERSION     DAJOWB010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.423"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..622
                     /locus_tag="OHO85_005170"
     CDS             <1..622
                     /locus_tag="OHO85_005170"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCQ2674357.1"
                     /translation="IRVVNNGCNFFYTDGDFYEKKNSVLKIITVGGIKPHKNIHLIPI
                     ISSKLNEIGVKHEWNIIGPVMNENYFKNVECINKKYTSSIIFRGEVCSQKLKEYYLSS
                     DIYVHLSKEEGFCLTILEAASFGLPCVGTNVGAIPEIISSLNNGILVEPDANVIYSAI
                     LKFNKDRNLYNHSRSLCDKTFALWGWDRVVKEYISHYNECIATFDK"
BASE COUNT          262 a           76 c          159 g          287 t
ORIGIN      
        1 cattagggtt gtgaacaatg ggtgcaactt tttttatact gatggagatt tttatgaaaa
       61 aaaaaatagt gttttaaaaa ttataaccgt aggaggtatt aaacctcata agaatattca
      121 tttaattcct ataatatcgt caaagctcaa tgagataggg gtgaaacatg aatggaatat
      181 tatagggcct gtgatgaatg aaaattattt taaaaatgtt gaatgtatta ataagaagta
      241 tacttcaagt attatattta gaggggaagt ttgctctcaa aaattgaagg aatattattt
      301 gagttctgat atatatgttc atttatcaaa agaagagggt ttttgtttga ctattttaga
      361 ggcggctagt tttggattgc cttgtgtggg gactaatgtt ggtgctatac ctgaaattat
      421 tagtagtctg aataatggta ttttagttga gccagacgca aatgttattt attcagcaat
      481 attgaagttt aataaagata gaaatttgta taaccactct aggagtttgt gtgataagac
      541 atttgcttta tgggggtggg atagagttgt taaagaatat atttctcact ataacgaatg
      601 tatcgctact tttgataaat aaaacgaaag ttaaaatgta tgaaaattca ttgcaggact
      661 gttgttggtt atattatagc atgtacagta tttagttctc tattaactat agaaacaggc
      721 attggtggat ttgagtttta ttattacccg ataatgacgt ttttgttttt tatagcgctc
      781 caaa
//
LOCUS       DAJOWB010000414          784 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJOWB010000414 DAJOWB010000000
VERSION     DAJOWB010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..784
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.424"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..591
                     /locus_tag="OHO85_005171"
     CDS             <1..591
                     /locus_tag="OHO85_005171"
                     /inference="COORDINATES: protein motif:HMM:NF012744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCQ2674358.1"
                     /translation="GVMYLEKHTKKSVFHLGIFSRISKNKGQLEFLNAISSVKNISII
                     IHLFGDVVHGEEEYFEKIKIKKSELECNEVQVLIHGYTSEPIDMMKGMDIVVLPTIKP
                     EPFGRVIIEAQMLGKIVVANNKGGPCEIICNGVNGFLANDVSQYASIIENIISNWDSM
                     DLISTNAKKSVAENFTVEKMTTKVNEVLSSYENSAL"
BASE COUNT          277 a           95 c          160 g          252 t
ORIGIN      
        1 ggcgtaatgt atttagaaaa acacacaaaa aaaagtgtat tccatcttgg aattttttcc
       61 agaatatcta aaaataaagg tcagttggag tttttgaatg ctatatcttc cgtaaaaaac
      121 attagcatta ttattcattt gtttggggat gtggttcatg gagaagaaga atatttcgaa
      181 aaaattaaaa taaagaagag cgagttagaa tgtaatgagg tacaagtgtt aattcacggg
      241 tacacttctg aacctataga tatgatgaaa ggaatggata tcgttgtatt gccaactata
      301 aaaccagaac catttggacg agtaattatt gaggctcaga tgcttggtaa aatagttgta
      361 gcaaataata aaggcggtcc atgtgaaata atatgtaatg gggtcaatgg ttttttggct
      421 aatgatgtga gtcaatatgc atctataatt gaaaatatca tatcaaattg ggattcaatg
      481 gatttaatat ctactaatgc aaagaagtct gttgctgaaa actttaccgt ggaaaaaatg
      541 acaacgaaag tgaatgaggt attgagttct tatgaaaatt ctgcacttta gtaaatatta
      601 tcctccctat tttggaggca ttgagactgt tgcttatgat atagttgaag gagtaaacga
      661 aaacaatatt tcttgtgatg tactgtgctt caatagtaat ctattttcag aaaccgaagt
      721 aaagaataag tatagagtct ttaggatggg gcgtttgttc gaaattttct ccacgcctat
      781 ttca
//
LOCUS       DAJOWB010000415          776 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJOWB010000415 DAJOWB010000000
VERSION     DAJOWB010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.425"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..573
                     /locus_tag="OHO85_005172"
     CDS             <1..573
                     /locus_tag="OHO85_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HCQ2674359.1"
                     /translation="NRHRYYDPLQGRYITQDPIGLKGGWNLYTYPLSPVNSMDPLGLY
                     EFKSKNIDDIGIFALAMCNGESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRN
                     IKCPEGSERVGDYHTHGFYSDDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMEKKEYS
                     SYLGTPNNTYLKYNPKAKGNGVTIIRQGSN"
     gene            575..>776
                     /locus_tag="OHO85_005173"
     CDS             575..>776
                     /locus_tag="OHO85_005173"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674360.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSNSDSAALYCVAKIF
                     PDGENILIYKVNIINASSKKDVF"
BASE COUNT          286 a          101 c          160 g          229 t
ORIGIN      
        1 aaccgccacc gctattatga cccgctgcag gggcggtata tcactcagga tccgattggg
       61 ctgaaggggg gatggaacct gtatacatat ccgctgagcc cggtgaatag catggatcca
      121 ttaggtttat atgaatttaa atcaaaaaat atagatgata ttggaatatt tgcattggca
      181 atgtgtaatg gagaatcaat taacgagaat aaagaatatg gtggactaat atgtaagaag
      241 caaggtgaat atttccccat gaatccgata agttcaaatg ataatgatag tgtagacttg
      301 cgaaatataa aatgccctga aggttcagag agagtaggcg attatcacac tcacggtttt
      361 tactctgacg ataaaggaaa taaagtaaca aaagaaaatg atgtttatga tagtctaaat
      421 ttttcaagca aagatttaac gaattcttat atgaatggaa tggaaaaaaa agaatacagt
      481 agttacttgg gaacaccaaa taacacctat ctaaaatata atcccaaagc taaagggaat
      541 ggagttacaa ttatcaggca agggagtaat taaaatgaag gcaatacaat gttgttattt
      601 ccttgcttta gtctccagtc atttaatggc tggagttatg ggtgatgata atatattttt
      661 aagtaattcg gattcagctg ctttatattg tgtggctaaa atatttccag atggagaaaa
      721 tattttaata tataaagtaa atataattaa tgctagttca aaaaaagatg tttttt
//
LOCUS       DAJOWB010000416          775 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJOWB010000416 DAJOWB010000000
VERSION     DAJOWB010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.426"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          201 a          200 c          202 g          172 t
ORIGIN      
        1 cggtttagct cagttggtag agcgcctgcc ttgtaagcag gatgtcagcg gttcgagtcc
       61 gttaaccggc accaacacaa caggtaaggg tattttgcga cgtcggagat cgccgtgctt
      121 ggcagagggt tcgaatccct acgaagtacc cttaccgttg tgatgaagtg cagctctttg
      181 aagcaaccag aagataagca tctggcttca caacataaac cgcaggaacg accaataaac
      241 ggtagtccgt atggagaaca ccccgttgag gaagaggcct ggccggaacc gtaaccggca
      301 ctacaacgtt gagaacactg gcgtaacggg gtcatatccc aatctacgaa taaatgttgc
      361 gttgcagcgt gacaaccagt gttctcaaca ttgtggtgaa tgcacaggct gatgtgccgc
      421 aactacagta gtgcgcgctt tgcggggctt gctacaaccc tgtgtcggag ttcagcaccg
      481 accatcacag tttgattctc tggcatgagc ataacgctga aataagtcca gtctggtgcg
      541 gcccgatcac ccgccgttag ctccacgaaa cggagcacgt aacaggtaag agcattctcc
      601 tgtaacgggt tcatatccca atctacaggt ccaccaggaa tgctctttcc gttgcggtga
      661 atgcggctaa gcgcacgcgg aacagttaaa acaatcctcc ttaatggtta aacattccgg
      721 cgttaattgt taactggtta acgtcaccgg gaggcactcg gcaccgcaat ctaat
//
LOCUS       DAJOWB010000417          773 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJOWB010000417 DAJOWB010000000
VERSION     DAJOWB010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.427"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>773
                     /locus_tag="OHO85_005174"
     CDS             <1..>773
                     /locus_tag="OHO85_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCQ2674361.1"
                     /translation="TLDVRNGGTATTVSMGNGGVLLADSGAAVSGTRSDGKAFSIGGG
                     QADALMLEKGSSFTLNAGDTATDTTVNGGLFTDRGGTLAGTTTLNNGAILTLSGKTVN
                     NDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLTQKAVNLNEGTLTLNDSTV
                     TTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIPDNATVQSVVDDLSHAGQI
                     HFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNA"
BASE COUNT          191 a          235 c          218 g          129 t
ORIGIN      
        1 acgctggatg tccgcaacgg tggcaccgcc accaccgtat ccatgggaaa tggcggtgta
       61 ctgctggccg attccggtgc cgctgtcagt ggtacccgga gcgacggaaa ggcattcagt
      121 atcggaggcg gtcaggcgga tgccctgatg ctggaaaaag gcagttcatt cacgctgaac
      181 gccggtgata cggccacgga taccacggta aatggcggac tgttcaccga caggggcggc
      241 acactggcgg gcaccaccac gctgaataac ggcgccatac ttaccctttc cgggaagacg
      301 gtgaacaacg ataccctgac catccgtgaa ggcgatgcac tcctgcaggg aggctctctc
      361 accggtaacg gcagcgtgga aaaatcagga agtggcacac tcactgtcag caacaccaca
      421 ctcacccaga aagccgtcaa cctgaatgaa ggcacgctga cgctgaacga cagtaccgtc
      481 accacggatg tcattgctca gcgcggtaca gccctgaagc tgaccggcag cactgtgctg
      541 aacggtgcca ttgaccccac gaatgtcact ctcgcctctg gtgccacctg gaatattccc
      601 gataacgcca cggtgcagtc ggtggtggat gacctcagcc atgccggaca gattcatttc
      661 acctccaccc gcacagggaa gttcgtaccg gcaaccctga aagtgaaaaa cctgaacgga
      721 cagaatggca ccatcagcct gcgtgtacgc ccggatatgg cacagaacaa tgc
//
LOCUS       DAJOWB010000418          769 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJOWB010000418 DAJOWB010000000
VERSION     DAJOWB010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.428"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(32..106)
                     /locus_tag="OHO85_005175"
     tRNA            complement(32..106)
                     /locus_tag="OHO85_005175"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(72..74),aa:Asn,seq:gtt)
BASE COUNT          180 a          231 c          184 g          174 t
ORIGIN      
        1 ccgctacccc tacggactaa ctcccctaaa gtggcgatgg tgggtggatt cgaaccaccg
       61 accagttggt taacagccaa ctgctctacc gctgagctac accatcactt gccgggtacg
      121 tctccggcga gggcttccac ctccgtatgc ttttcggcgc accgcgccct ggctgcaatt
      181 cggtaacagg ggatgcacaa ccctggcttc cagcgtgatt agcgccttca gcatgacggg
      241 atatacccgt aaattcgtgg aactgtaccc aaagtgctgt taagcaccgc tgttacgctg
      301 aaaagaaaac gcaacaggaa aggacgctga ccaacagatg gccccttctc gttcatctgg
      361 ttaatcacac cagcgccctt acctgttgtg cctccccgtt ccctaataca cagacgggga
      421 cactctgcgg tcgatttttt tgacggggga cgactcatac cccgtggcat ctggtttctt
      481 aggccgctac catcatcaga tcatcgtttg catttacttt aatggtcagt ttctaaaccg
      541 ccgcaaagtc gctaaccatg acgaaaaccc tgaaaaaaac gcccacccga agatgggcaa
      601 actggaagct cgtaacgcac ttcggcgttg ccacttaggc gcatggtcaa cctggcaact
      661 cggtggtttg tctgggagga ctaggcccag ccatgcttac cgccgcgcct gtcgcggcta
      721 acagctaaat cgctctataa atcacgattc attgaggcga tattacact
//
LOCUS       DAJOWB010000419          768 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJOWB010000419 DAJOWB010000000
VERSION     DAJOWB010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.429"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(61..723)
                     /locus_tag="OHO85_005176"
     CDS             complement(61..723)
                     /locus_tag="OHO85_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCQ2674362.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFGFSMDGAATEHD
                     TLLPRFTDDIHNQSWVGEKVFCNPPYSDIPSFLLKSSESDLAVFLIPYRPHTTYWLEH
                     IFTNPLCHEIRILHRAVKYLPPAGHNGLVVRAPFPAAIVIFRTEPRKHEITQMVCCAD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKCIADALRMPLSTSYRIAQR
                     LS"
BASE COUNT          220 a          155 c          175 g          218 t
ORIGIN      
        1 cagggatact tcttaatatc tgaatcacct gtcttgtaaa tgaccatatt tatgagaatt
       61 ttagctcaac ctttgagcaa tcctgtatga cgtgctcaga ggcattctga gagcatctgc
      121 aatgcactta atgggtttgc cttgcctgta tagttttatg aagctgtcga gcgtctcagg
      181 tgggtaaatg gtgggtcttc ctcttaagcc tcctctgtta atgatggtta acggtaatag
      241 cgtgtcagca cagcaaacca tctgcgtaat ttcatgctta cgtggctcag ttctaaagat
      301 gacaattgca gcaggaaacg gcgcacgaac aacaagcccg ttatgccctg ctggtggaag
      361 gtatttaact gcacgatgaa gaatacggat ttcatggcaa agcgggtttg tgaagatgtg
      421 ctcaagccag tatgtggtgt ggggtcggta cggtatcagg aataccgcta aatctgattc
      481 agacgatttt aagaggaacg acggaatatc gctgtatggt gggttacaga ataccttttc
      541 accaacccat gactgattgt ggatatcatc ggtgaaccgt ggtaacagcg tgtcatgctc
      601 agttgcagca ccatccatcg aaaaaccaaa ttcacggtgt aggttcttaa ataacaaaaa
      661 tggagttctc cagtaatcag aacccccatg atgcttcccg attaaaatat gctcaattga
      721 cataagaaat aaaaattttt attgcaatca ctcttgacaa agtttact
//
LOCUS       DAJOWB010000420          763 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJOWB010000420 DAJOWB010000000
VERSION     DAJOWB010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.430"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>763
                     /locus_tag="OHO85_005177"
     CDS             <1..>763
                     /locus_tag="OHO85_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2674363.1"
                     /translation="TARTIGGVSFNGTANIDLPGVNKTGNQSTTGNAATATKLQTART
                     IGGVSFDGSANINLPGVNIAGNQNTTGNAATATKLLTARTINGVSFDGSANISLSPAN
                     IGCPASPTGWLVTGDNGASITTEQLVTLLRDNGAFNAKAWIARCAWAYANSASIPDSE
                     TGCGIIPLAGAVIEVFNNGSSSNNYTIRITTATTTGVSGALTNAEFVYVFNGTSYSPG
                     WRRAYNTKNKPTAADVGALPLSGGALTGGLTAAGEI"
BASE COUNT          221 a          172 c          187 g          183 t
ORIGIN      
        1 acggcaagaa ccattggtgg cgtttcattt aatggtaccg caaacatcga cctgccaggt
       61 gtgaataaaa caggtaatca gagcactact ggcaatgctg caactgcaac aaaactgcaa
      121 acagcacgca caataggcgg cgtatcattc gatggctccg ccaatatcaa tctgccgggt
      181 gtaaacattg ctggtaacca gaacacaacc ggtaatgcag ctacggctac caaattgcta
      241 acagcgcgta ccattaatgg ggtttcattt gacggttctg cgaatatttc actgtcacca
      301 gcaaatattg gttgcccggc atctcctact ggttggcttg taacaggtga taatggagct
      361 tcaataacaa cagagcaatt agtgactctg ctacgagata atggtgcttt taatgcaaaa
      421 gcatggatag cgcgttgtgc ctgggcgtat gcaaatagtg catccatacc agacagcgaa
      481 acaggatgcg gcattattcc actggctggc gcagttattg aggtatttaa taacggcagt
      541 agctcaaaca attatacgat tcgtataaca acggcaacaa cgacaggtgt ctctggcgca
      601 ctcactaatg cggagtttgt ctatgtgttt aacgggacga gctattcacc aggatggcgc
      661 agagcgtata acactaaaaa caagcctact gcggctgatg tcggagcttt accactatct
      721 ggaggagcgt taactggcgg attaactgct gctggcgaga tta
//
LOCUS       DAJOWB010000421          749 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.431, whole genome shotgun sequence.
ACCESSION   DAJOWB010000421 DAJOWB010000000
VERSION     DAJOWB010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.431"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..749
                     /locus_tag="OHO85_005178"
     rRNA            <1..749
                     /locus_tag="OHO85_005178"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          174 a          168 c          241 g          166 t
ORIGIN      
        1 atggagccga ccttgaaata ccacccttta atgtttgatg ttctaacgtt gacccgtaat
       61 ccgggttgcg gacagtgtct ggtgggtagt ttgactgggg cggtctcctc ctaaagagta
      121 acggaggagc acgaaggttg gctaatcctg gtcggacatc aggaggttag tgcaatggca
      181 taagccagct tgactgcgag cgtgacggcg cgagcaggtg cgaaagcagg tcatagtgat
      241 ccggtggttc tgaatggaag ggccatcgct caacggataa aaggtactcc ggggataaca
      301 ggctgatacc gcccaagagt tcatatcgac ggcggtgttt ggcacctcga tgtcggctca
      361 tcacatcctg gggctgaagt aggtcccaag ggtatggctg ttcgccattt aaagtggtac
      421 gcgagctggg tttagaacgt cgtgagacag ttcggtccct atctgccgtg ggcgctggag
      481 aactgagggg ggctgctcct agtacgagag gaccggagtg gacgcatcac tggtgttcgg
      541 gttgtcatgc caatggcact gcccggtagc taaatgcgga agagataagt gctgaaagca
      601 tctaagcacg aaacttgccc cgagatgagt tctccctgac cctttaaggg tcctgaagga
      661 acgttgaaga cgacgacgtt gataggccgg gtgtgtaagc gcagcgatgc gttgagctaa
      721 ccggtactaa tgaaccgtga ggcttaacc
//
LOCUS       DAJOWB010000422          730 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJOWB010000422 DAJOWB010000000
VERSION     DAJOWB010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.432"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..338
                     /locus_tag="OHO85_005179"
     CDS             <1..338
                     /locus_tag="OHO85_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCQ2674364.1"
                     /translation="SKTSDLEEDKISDVNVDSDHKNTQCPATDDEMDPFDDYLDEESE
                     REKAIKRVEKKKRFELARQKNKVSGLDPFDDSDDDDFSFTPRKKQTQGDKKQKIKPKM
                     YFAGGGIKQ"
     gene            335..631
                     /locus_tag="OHO85_005180"
     CDS             335..631
                     /locus_tag="OHO85_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674365.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGCLMKDTHGEVYRIRPKKEGR
                     DYYFVDGVTDLKIDALRFAVMYHYDLWDSVHQLRLKDGDPGNLRATNIIKGKCR"
BASE COUNT          250 a          120 c          172 g          188 t
ORIGIN      
        1 cttctaaaac atctgactta gaggaggata aaatctctga tgtgaatgtt gatagtgacc
       61 ataaaaacac acagtgcccc gccactgatg atgagatgga ccccttcgat gattaccttg
      121 atgaagagtc agagcgtgaa aaagccatta agagggtaga gaagaaaaag agattcgagc
      181 ttgcaagaca aaagaacaaa gtatcgggtt tagacccatt cgatgacagt gacgatgatg
      241 acttttcttt cacaccaaga aagaaacaaa ctcagggtga taagaagcaa aaaattaaac
      301 cgaagatgta ttttgctggt ggagggatta agcaatgaaa gaatatcacg gtgaaaagag
      361 atacaaggat tacctgttaa agcgttacag catttctcgt gaaggttgct tgatgaaaga
      421 tactcacggg gaagtttacc ggattagacc aaagaaagaa gggagggatt attatttcgt
      481 cgatggtgtg acagatttaa agattgacgc tttgaggttt gcagtgatgt atcattatga
      541 tttatgggat tcagtccacc agctacgatt aaaagacggt gaccccggta atctgagggc
      601 caccaatatt atcaagggaa agtgccggta aggccaccag ctatttattt actgaaatct
      661 ctatctcaac acgaagaagt ttaagtcgaa ttgtaagctt tgtctttttt gtaatgactc
      721 gattcataac
//
LOCUS       DAJOWB010000423          729 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJOWB010000423 DAJOWB010000000
VERSION     DAJOWB010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.433"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     repeat_region   134..589
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=134..162
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT          194 a          174 c          185 g          176 t
ORIGIN      
        1 taaatattta cctttttaat caatgaataa agtactcttt aacataatgg atgtgttgtt
       61 tgtgtgatac tgtaaagttg gtagattgtg actggcttaa aaaatcatta attaataata
      121 ggttatgttt acagagttcc ccgcgccagc ggggataaac cgtccacgct gtaacggcca
      181 tcattaagtt tagtgagttc cccgcgccag cggggataaa ccggctgatg gtctgggagt
      241 gtccatcggg caactgagtt ccccgcgcca gcggggataa accggaagta ggcctgacag
      301 tgattgaacg catactgagt tccccgcgcc agcggggata aaccgagttg gggcggcgca
      361 ataacgagac gatacgcgag ttccccgcgc cagcggggat aaaccgggga gtggcacttc
      421 tggggtagcg gcggccctga gttccccgcg ccagcgggga taaaccgtca acgcgctcag
      481 acgttgcgtg agtgaaccag agttccccgc gccagcgggg ataaaccgcc cggaatgcat
      541 tctgaaggtt tgctgtatat gagttccccg cgccagcggg gataaaccac catataaccc
      601 gttatctctt tctcaagttt ttatattagc tgtacttgta ataagcaaca tatccacgta
      661 acacctcatg ttcaaaatag ttccccatgc cagataagtt cacaattatc gatacaaaaa
      721 atcaaattt
//
LOCUS       DAJOWB010000424          728 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJOWB010000424 DAJOWB010000000
VERSION     DAJOWB010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.434"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..88
                     /locus_tag="OHO85_005181"
     CDS             <1..88
                     /locus_tag="OHO85_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HCQ2674366.1"
                     /translation="KREYKKQEGNPEIKSKRRERIRKFFLSN"
     gene            115..477
                     /locus_tag="OHO85_005182"
     CDS             115..477
                     /locus_tag="OHO85_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HCQ2674367.1"
                     /translation="MIANPTHIAIGIYFKPHLSPIPLISVRETNEVALAVRKYAKEIG
                     IPIITDKKLARKIYATHRRYDYVSFENIDEILRLLLWLEDVENAGQPVPDEQLSSEDK
                     FIEGEEKEIENKDDNLKN"
     gene            complement(539..>728)
                     /locus_tag="OHO85_005183"
     CDS             complement(539..>728)
                     /locus_tag="OHO85_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCQ2674368.1"
                     /translation="RSGESQAIVDARQFFSEHPEYISSAELEQKLYREFAQVTTVPAY
                     KEMSMYQLLVISQDRLTH"
BASE COUNT          258 a          127 c          136 g          207 t
ORIGIN      
        1 taaaagagaa tacaagaagc aagaaggaaa tccagaaatt aagtctaaac gcagagagcg
       61 catcaggaaa ttctttctga gcaattgaag tctgatgtca gtaatagtcg tttgatgatt
      121 gccaacccta ctcacattgc aataggaata tactttaagc cacatctttc acctattcca
      181 ttgatttctg taagagaaac taatgaggta gccttagccg taaggaaata tgcaaaggaa
      241 atcggaatac caattatcac agataaaaaa ttagcacgaa aaatttatgc tacccatcgt
      301 cgctacgatt atgttagctt cgaaaatata gatgaaatat tacgtcttct gctgtggctt
      361 gaagatgtgg agaatgctgg acaacctgtt ccagatgaac agctctcttc agaagataaa
      421 ttcattgaag gtgaagagaa agaaatcgag aataaagacg ataatttaaa aaattaaaca
      481 tctgtcgtag tcagaaattt ataagaatct acacgcaaaa accgtgtaga ttcattattt
      541 aatgagttaa acgatcttgt gaaatgacaa gcaattgata catactcatc tctttataag
      601 ctggaactgt agtaacctgt gcaaattcgc gatatagctt ttgctccagt tctgctgaag
      661 atatatattc cgggtgttcg ctaaagaact gtcttgcatc tactatcgct tgggattcac
      721 cacttctt
//
LOCUS       DAJOWB010000425          722 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJOWB010000425 DAJOWB010000000
VERSION     DAJOWB010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.435"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..704)
                     /locus_tag="OHO85_005184"
     CDS             complement(<1..704)
                     /locus_tag="OHO85_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCQ2674369.1"
                     /translation="MATAWYKQVNPPQRKALFSAWLGYVFDGFDFMMIFYILHIIKAD
                     LGITDIQATLIGTVAFIARPIGGGFFGAMADKYGRKPMMMWAIFIYSVGTGLSGIATN
                     LYMLAVCRFIVGLGMSGEYACASTYAVESWPKNLQSKASAFLVSGFSVGNIIAAQIIP
                     QFAEVYGWRNSFFIGLLPVLLVLWIRKSAPESQEWIEDKYKDKSTFLSVFRKPHLSIS
                     MIVFLVCFCLFGANWP"
BASE COUNT          251 a          153 c          119 g          199 t
ORIGIN      
        1 atcggccagt ttgcaccaaa tagacaaaaa cagacgagga aaacgatcat agagattgaa
       61 agatgtggtt ttctgaagac agacaaaaat gttgatttat ccttatattt atcttcaatc
      121 cactcctgac tttctggagc actttttctg atccaaagaa ctagtaaaac tggtaacagg
      181 cctataaaaa aagagtttct ccatccatat acttcagcaa actgagggat tatttgtgcc
      241 gcaataatat ttccaacaga aaaaccactt accaaaaaag cactagcttt agattgaaga
      301 tttttaggcc aactttctac cgcataagtt gaagcacatg catattcacc agacatccct
      361 aagccaacaa taaaacggca aactgcgagc atatataagt ttgtagcaat accgctaagg
      421 cctgttccga ctgagtaaat gaaaattgcc cacatcatca ttggcttacg accatattta
      481 tcagccatgg caccaaaaaa accacctcca ataggtctgg ctatgaaggc cactgtccct
      541 attaaagtag cctgaatatc cgtaatgcca agatctgctt ttataatatg aagaatgtaa
      601 aatatcatca taaaatcaaa gccatcaaat acatatccaa gccatgcgga aaaaagagct
      661 ttccgttgtg gtggattaac ttgtttatac catgctgttg ccatatttgt ttcctgtgtt
      721 at
//
LOCUS       DAJOWB010000426          722 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.436, whole genome shotgun sequence.
ACCESSION   DAJOWB010000426 DAJOWB010000000
VERSION     DAJOWB010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.436"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(195..383)
                     /locus_tag="OHO85_005185"
     CDS             complement(195..383)
                     /locus_tag="OHO85_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCQ2674370.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDRNPFGAKCIFCKSMMITF
                     DNISQYLNVSHLSLDLRK"
     gene            complement(394..606)
                     /gene="cspI"
                     /locus_tag="OHO85_005186"
     CDS             complement(394..606)
                     /gene="cspI"
                     /locus_tag="OHO85_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HCQ2674371.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT          241 a          141 c          124 g          216 t
ORIGIN      
        1 gccattacag tgaggtgtgt tgtttcgcca tttggatatt cacgcatgat ggtgttaact
       61 ccggtcatca ctggcactac cattgctggt tcagagttaa aaaaaactat tatttttttc
      121 ataatgttac cgtaatatgt gagtatccat cgactagaca ctaagcaaaa aagctcccga
      181 aggagccttc attttcactt tcttaaatct aacgacagat ggctaacatt taagtattgt
      241 gaaatattat caaatgtaat catcattgat ttacaaaaga tacattttgc cccgaaagga
      301 tttctgtcag aaacatcaaa agatgatgtt ctatactgag aaccatggca acacgggcat
      361 ctaaagtgaa tatggtttgt aatattttct acctcaaagt gccactacat gaacagctgc
      421 agggccttta ggtccgttct caataccaaa ttcgacttcc tgattctcag ttaacgtttt
      481 gaaatcgttg ctctgaatag ctgagaaatg cacaaacaca tctttgctac catctttcgg
      541 cgtgatgaaa ccaaaacctt tttcagggtt aaaccatttc actaaaccag tcattttgtt
      601 agacatcatt attacctttt gaagaagtta gcccttgggc agaatggtcc gaaaaaaata
      661 tcagagagaa aaaccaacaa ggaaatctca agaggtacaa ataataaaat tataacaatg
      721 ac
//
LOCUS       DAJOWB010000427          713 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.437, whole genome shotgun sequence.
ACCESSION   DAJOWB010000427 DAJOWB010000000
VERSION     DAJOWB010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.437"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            137..628
                     /locus_tag="OHO85_005187"
     CDS             137..628
                     /locus_tag="OHO85_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCQ2674372.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
BASE COUNT          279 a          112 c          112 g          210 t
ORIGIN      
        1 acttttacca ataatgaaaa atataccata agcaatgcaa gcttaaaact cataatttaa
       61 aaaagataaa tataaaaata tcaatgagtc aatcaataat aatttggcat cacgagacac
      121 atcacagagg aatattatga gcacagaaac aattgaaata ttcaataata gtgatgaatg
      181 ggcaaatcaa ctaaaacacg cattatcgaa aggagaaaat ctggcattac tacatggttt
      241 aactcctgat atccttgata gaatatatgc atatgcattc gactaccatg aaaagggtaa
      301 tataacggac gcagaaattt attataaatt tttgtgcatt tatgcgttcg aaaatcatga
      361 gtatctaaaa gattttgcat cagtatgtca gcccaaaaag aaatatcaac aagcatatga
      421 cctttacaaa ctaagttaca attactcccc gtatgatgac tattcagtta tttatcgtat
      481 gggtcaatgt cagattgggg ctaaaaatat cgataacgca atgcaatgtt tctatcacat
      541 tattaacaat tgtgaggatg atagtgttaa gagtaaagcg caggcatata ttgaactctt
      601 aaacgataat tcagaagata atggctaagc attacaatca tttggttaaa tttgctggtt
      661 atagtgcagt taaccactat aaccagcacc tcttatgttt tcttaaatca gca
//
LOCUS       DAJOWB010000428          710 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.438, whole genome shotgun sequence.
ACCESSION   DAJOWB010000428 DAJOWB010000000
VERSION     DAJOWB010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.438"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..390)
                     /locus_tag="OHO85_005188"
     CDS             complement(<1..390)
                     /locus_tag="OHO85_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674373.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYHGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECI"
     gene            complement(646..>710)
                     /locus_tag="OHO85_005189"
     CDS             complement(646..>710)
                     /locus_tag="OHO85_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCQ2674374.1"
                     /translation="INVNFFSSDRTSFCECNRFP"
BASE COUNT          211 a          139 c          134 g          226 t
ORIGIN      
        1 aatacattcc agctcttttt tattgacggc tgagcaagta cattgttcac caaaaggtgc
       61 aaaagtgcct acatctaatg tttgtatgat ataatcatca tgttctcctg tagaaatatc
      121 tataatgaat ggactcatat tttccggttg gattcggatc atgacagaag gaacatcgcc
      181 atgatagaaa tttaatgcag ttattgcaga taatactctc cgcagttcat tacttatact
      241 gtaaatttta ttaacgtcat tggtggagac aatttctgca aactttcttt ttaccgtcca
      301 ataatcaata tttctattgt gattgttaac cactgcgtgc attggcggga ttgaagggtg
      361 aaaaatatgc gcaattggga agcctttcat tgatgcctgc cttgtcatca tgacaaacaa
      421 taatagatgc ttattttcct cgcagggttc tttcgcacta tcaaaaaacg ctcatttacc
      481 cggcgttaca gtaaacgaag caacattctg tggcctacgc cgatcaaaag ctctgtgaaa
      541 atttcccttg ttgtctttgt ctgaccttat cgcggcgatc agcaattatt cgttttgtga
      601 tcctcaacac aaaatgctat tcgtcgccta tgaggggaga agaatttatg gaaaacgatt
      661 gcattcacaa aatgacgtcc tgtcagaaga gaaaaagttg acgttaatca
//
LOCUS       DAJOWB010000429          708 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.439, whole genome shotgun sequence.
ACCESSION   DAJOWB010000429 DAJOWB010000000
VERSION     DAJOWB010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.439"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            270..>708
                     /locus_tag="OHO85_005190"
     CDS             270..>708
                     /locus_tag="OHO85_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HCQ2674375.1"
                     /translation="MVMIPALDTSAHTTSALGRMFITQKSMILARDLGYRLEGTDSDA
                     LEVKKYKGRFPYLCFLDEVGAYYTDRIAVEATQVRSLDFALILMAQDQERIEGQTTAT
                     NTATLMQNTGTKFAGRIVSEGSTARTLKSAAGEEARARMNNLQR"
BASE COUNT          188 a          173 c          183 g          164 t
ORIGIN      
        1 ccaaacttta ctgtcgtggc ctcgatgatc agtggccgga agaagctatc gcaccgttac
       61 gtaactacct tcaggacgtt cccggtttcg acctgtcgct ggtcaggaca ccatcagcct
      121 ggacggaagt acccagaaaa caacatgctt atctctccgg gcagttttct gaaactttca
      181 gcacattcac ggaagcattc ggggatattt ttgctgaaga ttccggtgat atcgatatcc
      241 gtgacagcat tcacagcgac aggattctga tggtaatgat ccccgctctg gatacatcag
      301 cgcatacaac gtctgctctt ggacgaatgt ttatcaccca gaaaagtatg attctcgccc
      361 gtgatttggg ctaccgcctc gaaggaactg attccgatgc actggaagtc aaaaaatata
      421 aggggcgttt cccttatctg tgtttccttg atgaagtagg ggcatactat acggaccgta
      481 ttgctgtgga agccactcag gtgcgttcac tggattttgc tctgattttg atggcacagg
      541 accaggaacg tatagaagga cagacaacag ccacgaatac tgcaacactg atgcagaaca
      601 cggggacaaa atttgccggc aggattgtca gcgagggtag tacggccaga acgctgaaaa
      661 gcgccgccgg tgaagaagcc agggcgagaa tgaacaacct gcagcgtc
//
LOCUS       DAJOWB010000430          705 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.440, whole genome shotgun sequence.
ACCESSION   DAJOWB010000430 DAJOWB010000000
VERSION     DAJOWB010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.440"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(389..463)
                     /locus_tag="OHO85_005191"
     tRNA            complement(389..463)
                     /locus_tag="OHO85_005191"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(428..430),aa:Gly,seq:tcc)
     gene            complement(467..542)
                     /locus_tag="OHO85_005192"
     tRNA            complement(467..542)
                     /locus_tag="OHO85_005192"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(507..509),aa:Thr,seq:tgt)
BASE COUNT          220 a          170 c          132 g          183 t
ORIGIN      
        1 aaaaaaccgc cagatatggc ggttggtcaa tgtataagat aaatcatttt aattgtaata
       61 aaaatcgagg tgtcgggtgc ctcccgaaat atctgtccct acaacaaata ttgtgattcc
      121 ccgctaaacc actatataaa ccaccctcgc actgaggaac acctctgtgg tgcttttaca
      181 acaccagaat gatgcatcac cgaccctgcc aggaaataca aaatctccac cgataatgca
      241 ccattctgct gtcgtaaaaa aatcagcact gaggctacac ctggcctcaa atcatagcca
      301 gagaacagaa tgcttttcca aaacaacctg ctcccacgta ataaaaaata cgccagtgcc
      361 gcaatacaat aaggcttgtt tcaaatgctg gagcgggtag cgggaatcga acccgcatca
      421 tcagcttgga aggctgaggt aatagccatt atacgatacc cgcatatggt gccgactacc
      481 ggaatcgaac tggtgaccta ctgattacaa gtcagttgct ctacctgctg agctaagtcg
      541 gcattggttc ttcaggggag cgatatcacc gatcaaagaa gagttccccc tcagaaccgt
      601 tttcgataat acgatttaat attccaatcg caacaacact ttgcgtcaag ttatgtaaat
      661 ttatttatgt atttttattt tatgtgaaca tttcaccttc attta
//
LOCUS       DAJOWB010000431          702 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.441, whole genome shotgun sequence.
ACCESSION   DAJOWB010000431 DAJOWB010000000
VERSION     DAJOWB010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.441"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(18..659)
                     /locus_tag="OHO85_005193"
     CDS             complement(18..659)
                     /locus_tag="OHO85_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maturation control protein"
                     /protein_id="HCQ2674376.1"
                     /translation="MDKKICVVSMSVGKPASMTAAWINNELIMAERTSYPERRRDMEL
                     QLLRELREKEEKGFIVLVEEENSFITGRVGQRVRLRDPFMNGRPVLIEAMQIYKELER
                     QKAIKLPRKESGKYILHQSIFDSEHDKKGDEFFNINWSEITTEHVLTLLCCFATEYNN
                     VASADYIRAMAGEVEARQEPSLLSPLINIIRGTQTLAQKQVPKGVLTGKGNYL"
BASE COUNT          152 a          183 c          153 g          214 t
ORIGIN      
        1 ccctaatgca ttgacgttta tagataattt ccttttcctg ttaatactcc cttaggcacc
       61 tgtttttgtg ccagcgtttg ggttccgcga attatgttaa tcagagggct tagtaacgat
      121 ggttcctggc gtgcctcaac ttctccagcc attgccctga tgtagtctgc gctggcaacg
      181 ttgttgtatt ccgtcgcaaa gcaacatagt aacgtcagaa catgctctgt cgttatttcg
      241 ctccagttga tgttgaaaaa ttcatcgcct tttttgtcgt gttcggaatc gaagatgctt
      301 tggtggagga tgtatttgcc ggattctttg cgcggtaact tgatcgcttt ctggcgttcc
      361 aattccttgt aaatctgcat tgcttcaatc agtaccggcc tgccgttcat aaagggatct
      421 cgcaacctta cacgctggcc aactcgacca gtaataaagc tgttttcctc ttccaccagc
      481 acgataaaac ccttttcctc tttttctcgc aattcgcgca gcagctggag ttccatgtcg
      541 cggcggcgtt cagggtagct ggtccgctca gccattatca gctcattgtt gatccatgca
      601 gcagtcattg acgccggttt gccgacgctc attgaaacaa cgcatatttt cttatccata
      661 gcgccccaca aaaaagaaaa gccaccagcg gcggcttagc aa
//
LOCUS       DAJOWB010000432          701 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.442, whole genome shotgun sequence.
ACCESSION   DAJOWB010000432 DAJOWB010000000
VERSION     DAJOWB010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.442"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>701
                     /locus_tag="OHO85_005194"
     CDS             <1..>701
                     /locus_tag="OHO85_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCQ2674377.1"
                     /translation="TINYQVLREAAEKATPDEWVAFISTDTGTYAVHTPGDERCEDVI
                     KWTGFDGQKNAENNARHVAAFNPKVALELLGEIKCLEDTNIDAMCRIAELETNLAALV
                     AENAGLKAFKTAVYQQMGVGCDAPEFSITVGLSNLRRFADTLHAIEREFFTKELPDEE
                     HEGETFNECPLSWGMSVEQYVSEFRKCLAEVRAQGLDAAIEAAKNLVAQEYEYKDFKA
                     AQSDCCMHPGSDLVG"
BASE COUNT          189 a          145 c          201 g          166 t
ORIGIN      
        1 acaattaact atcaggtact gcgtgaggcg gcagaaaagg caacaccaga cgaatgggtc
       61 gcatttattt cgacggatac tggtacttat gcggtgcaca cgcccggtga tgaacgatgt
      121 gaagacgtta tcaaatggac cggctttgat ggacagaaaa atgcagagaa caacgctcgt
      181 catgttgccg cgttcaaccc aaaggttgca ctggagctgc ttggtgaaat taagtgcctg
      241 gaggacacaa atattgatgc tatgtgtcga attgcagagc ttgagactaa tctcgcggcg
      301 ctggtggcgg agaatgcggg gcttaaggcc ttcaaaaccg ccgtttatca gcagatgggc
      361 gtcggatgtg atgctcctga attctccatt acggtaggtt tgagtaactt acgtcgtttt
      421 gctgacacac tccacgccat tgagcgtgag ttctttacca aagagctacc tgatgaagag
      481 cacgaaggcg aaacattcaa tgaatgtcca cttagctggg gaatgagcgt cgaacagtac
      541 gtttccgagt ttcgcaaatg cctggctgaa gtgcgggcgc agggattgga tgctgctata
      601 gaagctgcaa aaaatctggt ggcccaagaa tatgagtata aggatttcaa agcggcgcag
      661 agtgattgct gtatgcaccc tggttcagat ctggtaggga a
//
LOCUS       DAJOWB010000433          700 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.443, whole genome shotgun sequence.
ACCESSION   DAJOWB010000433 DAJOWB010000000
VERSION     DAJOWB010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.443"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            357..506
                     /locus_tag="OHO85_005195"
     CDS             357..506
                     /locus_tag="OHO85_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HCQ2674378.1"
                     /translation="MEKVISHTRYKLTSAELEAFNSVVDHWLAELTMNKLYDRVPASV
                     WKYVI"
BASE COUNT          195 a          140 c          190 g          175 t
ORIGIN      
        1 catatatgtg aaataaacac ggtgagtgtg atcagtgggc atgatgagaa cgcagtagca
       61 acagactgcg ctatccctga tagctggcgt ataacccgcg tatataaaac gtatctgcaa
      121 tgtacgtgaa cagagtgcat taagcatccg tctgagcggt aaacatatcc gacctggtga
      181 gtgacagtct gagagaccgg ttaagtggtg gctcgggagt aatgagtttg gggtgggtac
      241 cagaatgcca gtctggcggt atgctgacag tatcacgtta attgcagcat aaagtgatga
      301 gaggctaatg gaagttaaaa ccaaagcagt tccgtcgttg ttcatcccgg gagacgctgg
      361 aaaaagtgat ttcccacacg cgttataaac ttacctctgc ggaactggaa gccttcaact
      421 ctgtggttga tcactggctg gcagaactga caatgaacaa gctttacgat cgtgttcccg
      481 cttcagtctg gaaatatgtc atctgagcgt tcggtaatca caactgcatc gcaccaggtg
      541 ttatccgctt tgtgattctt attggctctt ctcaggttat taaaagctga atatgttcct
      601 gttgtcggga accaggataa taccgggaac gttaagcggt atagcgacaa caaagaattc
      661 gaaatcaaaa gggaatggtg ccggaggcgt gggcggtaat
//
LOCUS       DAJOWB010000434          681 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.444, whole genome shotgun sequence.
ACCESSION   DAJOWB010000434 DAJOWB010000000
VERSION     DAJOWB010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.444"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..346
                     /locus_tag="OHO85_005196"
     CDS             <1..346
                     /locus_tag="OHO85_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2674379.1"
                     /translation="GAHTHSVSGKAASAGAHTHTVACYPSGNTEQGDKALKGASRSGS
                     STGTSSAGAHTHTVSGTAASAGAHAHTVDIGAHTHSVAIGSHGHTITVNAAGNAENTV
                     KNIAFNYIVRLA"
     gene            346..>681
                     /locus_tag="OHO85_005197"
     CDS             346..>681
                     /locus_tag="OHO85_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCQ2674380.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSEDTSWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     DGEYQKWNGT"
BASE COUNT          179 a          172 c          172 g          158 t
ORIGIN      
        1 cggggcgcac acccatagtg tcagtggtaa ggctgcttct gcaggggcac atacacacac
       61 ggttgcctgt tacccatctg gcaataccga gcagggcgat aaagccctca aaggtgcgtc
      121 tcgttcaggc tcgagtactg gcacatcgtc agccggagcg catacccaca cggtgtcagg
      181 tactgctgca agtgcaggcg cacacgcaca tactgttgat attggcgctc atacacactc
      241 cgttgcgatt ggttcacatg gacacaccat caccgttaac gctgctggta acgcggaaaa
      301 caccgtcaaa aacatcgcat ttaactatat tgtgaggctt gcataatggc attcagaatg
      361 agtgaacaac cacggactat aaaaatttat aatctgctgg ccggaaccaa tgaatttatt
      421 ggtgaaggtg atgcatacat tccccctcat acaggtctgc ctgcaaacag taccgatatt
      481 gcaccgccag atattccggc tggcttcgtg gctgttttta acagtgagga tacatcatgg
      541 catctcgttg aagaccacag gggaaaaacg gtctatgacg tggcatcagg tgacgcttta
      601 tttatttctg aactcggccc gttaccggaa aatgtcacct ggttgtcgcc ggatggggaa
      661 tatcagaagt ggaacggcac a
//
LOCUS       DAJOWB010000435          679 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.445, whole genome shotgun sequence.
ACCESSION   DAJOWB010000435 DAJOWB010000000
VERSION     DAJOWB010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.445"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            1..679
                     /locus_tag="OHO85_005198"
                     /pseudo
     CDS             1..679
                     /locus_tag="OHO85_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          163 a          170 c          199 g          147 t
ORIGIN      
        1 ctatttctct ggcgcaagca ataccaggag ggaagtctta ctgctgtggc cgcaggagag
       61 caggtcgttc ctgcctctga acttgctgct gccatgaagc agattaaaga actccagcgc
      121 ctgctcggca aaaaaacgat ggaaaatgaa ctccttaaag aagccgttga atatgggcgt
      181 gcaaaaaagt ggatagcgca cgcgccctta ttgcccgggg atggggagta agcttagtca
      241 gccgttgtct ccgggtgtcg cgtgcgcagt tgcacgtcat tctcagacga accgatgact
      301 ggaaagatgg tcgccgcagc cgtcactccg ataatacgga tgtgcttctc cgtatacacc
      361 atgttatcgg agagctgccc acgtatggtt atcgtcgggt atgggcgctg cttcgcagac
      421 aggccgaact tgatggtatg cctgcgatca atgccaaacg tgtttaccgg atcatgcgcc
      481 agaatgcgct gttgcttgag cgaaaaaccg ctgtaccgcc atcgaagcgg gcacatactg
      541 gcaaagtggc cgtgaaagaa agcaatcaac gatggtgctc tgacgggttc gagttccgct
      601 gtgataacgg agaaaaactg cgagtcacgt tcgcgctgga ctgctgtgac cgtgaggcac
      661 tgtactgggc ggtcactac
//
LOCUS       DAJOWB010000436          679 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.446, whole genome shotgun sequence.
ACCESSION   DAJOWB010000436 DAJOWB010000000
VERSION     DAJOWB010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.446"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            28..519
                     /locus_tag="OHO85_005199"
     CDS             28..519
                     /locus_tag="OHO85_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCQ2674381.1"
                     /translation="MTLLFVAITYAAMELRGWFPKGSSTYLLMRETHYNAGIFVWVLM
                     FSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLGIALMAYSGKSWSF
                     LGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHHYIQKDNTLLRMMP
                     RRK"
BASE COUNT          198 a          152 c          132 g          197 t
ORIGIN      
        1 tcaaaaactc aaatttattt acattggata acgctgcttt tcgttgcaat aacctatgcc
       61 gcgatggaac tccgtggctg gtttcctaaa ggtagtagta cttatctgct gatgcgagaa
      121 acacattaca atgcgggtat attcgtttgg gtgttaatgt tttcacgcct gattataaaa
      181 caccgttata gtgatccttc tattgtgcca ccgccacctg cctggcaaat gaaagcggct
      241 tcgctaatgc acatcatgct ttatataacc ttccttgcac ttcctctgct ggggattgct
      301 ttgatggctt acagtggaaa atcgtggagt ttccttggtt tcaatgtgtc tccctttgtt
      361 accccaaaca gcgaaattaa agcactgata aaaaatattc acgaaacctg ggcaaatata
      421 ggctactttt taatcgcagc tcacgctggc gcagcactct ttcatcacta cattcagaaa
      481 gataatactc tgttacgaat gatgcctcgc cgcaaataag tcgatcccct gacaaggaaa
      541 tgtgaaccat ggacatcaac gcgtcataaa tgtttacgca agtgcacaat gcaccctttc
      601 cactatcaac aaggaggagc agaaactgtg taaatcgtac aatggtaaga cttatcaaaa
      661 atacaggtta attttggta
//
LOCUS       DAJOWB010000437          668 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.447, whole genome shotgun sequence.
ACCESSION   DAJOWB010000437 DAJOWB010000000
VERSION     DAJOWB010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.447"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..527
                     /locus_tag="OHO85_005200"
     CDS             <1..527
                     /locus_tag="OHO85_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCQ2674382.1"
                     /translation="PLAESFGLMPEIGAWVLGEACRQMHKWQGPAWQPFRLAINVSAS
                     QVGPTFDDEVKRVLADMALPAELLEIELTESVAFGNPALFASFDALRAIGVRFAADDF
                     GTGYSCLQHLKCCPITTLKIDQSFVARLPDDARDQTIVRAVIQLAHGLGMDVIFRRRL
                     HQLIGRNGCCAASS"
BASE COUNT          133 a          202 c          198 g          135 t
ORIGIN      
        1 tgccgctggc cgagtcgttc ggcctgatgc cggaaatagg cgcgtgggtg ctgggcgagg
       61 cctgtcgcca gatgcacaag tggcaaggac cggcatggca accgttccgt cttgccatca
      121 atgtgtccgc cagccaggtt gggccaacgt tcgacgacga ggtaaagcgg gtgctggccg
      181 atatggccct gcccgccgag cttctggaga tcgaactgac cgaatcggtc gcattcggca
      241 atccagccct gttcgccagt ttcgacgcct tgcgcgccat cggcgtgcgc ttcgccgccg
      301 acgacttcgg caccggctat tcctgcctgc aacatctgaa atgctgcccc atcaccacat
      361 tgaaaatcga ccaatccttt gtcgccaggc tcccggatga tgcccgtgac caaactatcg
      421 tgcgggcggt gatccagctc gcgcacgggc tgggcatgga tgtcattttc agaagacgac
      481 tgcaccagtt gattgggcgt aatggctgtt gtgcagccag ctcctgacag ttcaatatca
      541 gaagtgatct gcaccaatct cgactatgct caatactcgt gtgcaccaaa gcgaggtgag
      601 catggcgacg gacaccccac ggattccaga acaaggcgtg gccactctgc ctgatgaggc
      661 ttgggagc
//
LOCUS       DAJOWB010000438          660 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.448, whole genome shotgun sequence.
ACCESSION   DAJOWB010000438 DAJOWB010000000
VERSION     DAJOWB010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.448"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          194 a          176 c          124 g          166 t
ORIGIN      
        1 gtgtatagta ccattcccga cattctcaac atcttcagac atccatgcag actgagtgtt
       61 gatttcagtt tttcacggcc agcatggatc tttactgttt aaaaaacata cagatttact
      121 ctgaaattat ttacccaggc ctccctgaaa agaccatccg gagcatcccg ttcgtcagct
      181 tccatgttgt cttttacccc ggaaagtgat cgcattttcc agccagaatg acagccatac
      241 tgattatgac gtctctccgg ccccttcaca tataacattc atgtcagtca tttcttactg
      301 cctcactcac cggaattctc catgccggat gaagcaattg ttctgaactg agcagaagca
      361 taacagcaca caaggaaacg actgtgctat agatcctgga atcaggatct taatgtcagg
      421 aaaacaatga cgaccgggga taaccaacaa caacctgagt acagaagaaa gctttcccgc
      481 gcacgccatc atcttatgta agaggatatg atgccacccc cacctcagaa gaaccgtctc
      541 cgggagaact tcactgcatc caaaatcctt ttgctgagtt ctcattcccc atcatggcag
      601 ctacagacat gccctgacag agcgtatgcg cattgagaga aataaccata aatgatgaga
//
LOCUS       DAJOWB010000439          650 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.449, whole genome shotgun sequence.
ACCESSION   DAJOWB010000439 DAJOWB010000000
VERSION     DAJOWB010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.449"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>650
                     /locus_tag="OHO85_005201"
     CDS             <1..>650
                     /locus_tag="OHO85_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCQ2674383.1"
                     /translation="ASVRVHYYCGKGSFDEYRLKTLKRKAGWISDILRSDKSEMENAD
                     ANDMIEMQMYTAKDDGERLAMMQVQMDKAKAAKRARQKEQATIDLKNYIKAQHAAGED
                     VEVLTAELERSKAELEKTTAEVAKFKQAVMAKAADNADWKARWGSVHHTDRMLLAQYR
                     ASLKSAIQRKANISQAISRYEKLLNRTQKAATDIKRLRPLVEDAINKGILDVDPDL"
BASE COUNT          191 a          155 c          180 g          124 t
ORIGIN      
        1 tgcaagcgtt cgcgttcact actactgcgg caaggggtct ttcgatgaat accgactgaa
       61 gacgctgaag cgtaaagcag gctggatctc cgatatcctc cgttcagata agtcagaaat
      121 ggagaacgcc gacgccaatg acatgatcga aatgcagatg tataccgcga aggatgacgg
      181 cgaacgtctg gcaatgatgc aggttcaaat ggataaggcg aaagctgcga aacgcgctcg
      241 ccagaaagaa caggctacta tcgaccttaa gaactacatc aaggcgcagc acgcagctgg
      301 tgaggatgtg gaggtactta ccgctgaatt ggagcgaagc aaagcggaac ttgaaaagac
      361 caccgccgag gtagctaaat tcaaacaggc ggtaatggcc aaagcagctg ataacgcaga
      421 ctggaaagcc cgctggggta gcgtccatca cacagaccgt atgttgttag cacagtatcg
      481 cgcgtcgttg aaaagcgcca ttcagcgcaa ggctaatatc tctcaggcca tctcccgcta
      541 tgagaaatta ttgaaccgta ctcagaaggc cgcgacggat atcaaacgcc tgcgcccgct
      601 ggtggaggat gcaataaata aaggcattct ggatgttgat cctgatctgg
//
LOCUS       DAJOWB010000440          649 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.450, whole genome shotgun sequence.
ACCESSION   DAJOWB010000440 DAJOWB010000000
VERSION     DAJOWB010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.450"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>649
                     /locus_tag="OHO85_005202"
     CDS             <1..>649
                     /locus_tag="OHO85_005202"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCQ2674384.1"
                     /translation="TLLRQKELSASKARNVGLVKANSDIVLFFDDDVIIETNYLLKHH
                     LMNYADKDISGVFGQILLKNHYVTNVRSPYSYNERYGWLFFPSNYESRVIQRGIGMAG
                     NLSVRKKDAISIGGMDHNFEKGAYREESDFIQRFWNEGYKIVFDPMASLIHIGHKTGG
                     IRSWGASRGLCAPHHLRGEWYFILNHGLKTNILMNLYSLIRRQIINKENIRKPLN"
BASE COUNT          223 a           81 c          133 g          212 t
ORIGIN      
        1 ttactctttt aaggcagaaa gagctaagcg catctaaagc tagaaatgtt ggattagtaa
       61 aggctaactc agatatagta ttgttctttg atgatgatgt tatcattgaa acaaattatt
      121 tgttaaaaca ccatttaatg aattatgctg ataaagatat ttctggtgtt tttggacaaa
      181 ttttactaaa aaatcattac gttactaacg ttcgttctcc ttatagctat aatgagagat
      241 atggatggtt atttttccct tctaactatg aatcaagagt tattcagagg ggtataggaa
      301 tggcaggaaa tctttcagta agaaaaaaag atgctattag tataggtggg atggatcata
      361 attttgaaaa aggcgcatat cgagaggaaa gcgatttcat tcagcgattt tggaacgaag
      421 gatacaagat tgtttttgac cctatggcat cattaatcca tattggacat aagaccggtg
      481 gtattcgttc ttggggggca agtagaggtt tatgtgcgcc gcatcattta agaggtgagt
      541 ggtattttat tttaaatcat gggttaaaaa caaatattct aatgaatcta tactctttaa
      601 ttagacggca aataattaat aaggaaaaca taaggaaacc actgaatat
//
LOCUS       DAJOWB010000441          646 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.451, whole genome shotgun sequence.
ACCESSION   DAJOWB010000441 DAJOWB010000000
VERSION     DAJOWB010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.451"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..525)
                     /locus_tag="OHO85_005203"
     CDS             complement(<1..525)
                     /locus_tag="OHO85_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HCQ2674385.1"
                     /translation="MLRIKPLFILCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQD"
BASE COUNT          166 a          168 c          162 g          150 t
ORIGIN      
        1 gtcctgacag actttttgct gggacacctg cgtccggggg aaaagcccgc cgataatccc
       61 ctgcgccgcc tggcaggaac tgaggttcat gctgttaatg tcgcttgcca ttttctgcag
      121 gaagtctttt gcggttttga tttccggcac cgttgtctgc agggcaaggt caaaaaagta
      181 accggcagca ttactcataa tctgcttaac aaaacgctgc agctgctcac cattaataaa
      241 actgaacgaa ccgagataag cgtcgatgcc gccacatccg gcattgatat ccggcagcgt
      301 catggaaatc agctgaacat ttttgacctg agtacgggca tacagggagc cgccgtaagc
      361 atagccggca gcctgcccct gccagacgcc cggctgtgtg gtattggagg cgaagcccag
      421 cttgttaaag aactgattca tgtcgctgtt cacatccgct gacgctgcag gcgtcaccgt
      481 cagtaaggcg gcacaaagaa taaaaagagg cttaatgcga agcattattg ctgccctccc
      541 ttaatgtgtt ctcccggcgc atacggcgtt tcaacaggaa tatcccgata tttccgtata
      601 ttaattcgta cattgggaac agaaaaaatg taacaagaaa tccggc
//
LOCUS       DAJOWB010000442          644 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.452, whole genome shotgun sequence.
ACCESSION   DAJOWB010000442 DAJOWB010000000
VERSION     DAJOWB010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.452"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..>644)
                     /locus_tag="OHO85_005204"
     CDS             complement(<1..>644)
                     /locus_tag="OHO85_005204"
                     /inference="COORDINATES: protein motif:HMM:NF024180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HCQ2674386.1"
                     /translation="IKNELHRVIKECNDPSINSVVNEKVSKKGSYIPNFEMEVIFKDV
                     TNKNELVDSLNSFKFALVIFDMHGGHDYDGHGFLELSGEILYPYELMGLANIPPIVVL
                     SACDTSPADRNHFNAANAFLCAGAKTVLASTYPILSRDAAIYIGRLYKRLRYYLPERI
                     LFTKTSLRWSEFITGLNRRVYFDYFLMYIFRKYKINDKSILIELRNYINIALEN"
BASE COUNT          205 a          115 c           93 g          231 t
ORIGIN      
        1 tgattttcta atgcaatatt tatataattc cgtaactcta ttaatatgga cttatcatta
       61 attttatatt ttctgaaaat gtacattaaa aaataatcga aataaactcg gcggttaagt
      121 cccgtaatga actctgacca tcttaaactt gttttagtga ataaaattct ttcaggtaga
      181 tagtatctta ggcgtttata taatcggcct atatagatag ccgcatctct acttaatatg
      241 gggtatgtac tagccagtac cgtttttgct cctgcacata gaaaagcatt tgctgcatta
      301 aaatgatttc tatctgctgg gcttgtatca caagcactta atacgactat tggtgggata
      361 ttagctaaac ccattaactc ataaggatac aaaatttcac ctgatagctc aaggaatcca
      421 tgcccatcat aatcatggcc accgtgcata tcaaatatta caagagcgaa tttaaatgaa
      481 ttcaaagaat ccactagctc atttttattt gtgacatctt taaatattac ttccatttca
      541 aagttcggga tgtaagaccc ttttttagaa actttttcat ttactactga attaatacta
      601 gggtcgttgc attcttttat tacccgatgc aattcatttt ttat
//
LOCUS       DAJOWB010000443          640 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.453, whole genome shotgun sequence.
ACCESSION   DAJOWB010000443 DAJOWB010000000
VERSION     DAJOWB010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.453"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>640
                     /locus_tag="OHO85_005205"
     CDS             <1..>640
                     /locus_tag="OHO85_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674387.1"
                     /translation="WQYQNNERAIHSIMMSNKGDDLRSVFYVDGAAFPVFAFIEDGLS
                     ISAPGADLVVNDTTYKFGATNPATECIAADVILDFKSGRGFYESHSLIVNDNLSCKKL
                     FATDEIVARGGNQIRMIGGEYGALWRNDGAKTYLLLTNQGDVYGGWNTLRPFAIDNAT
                     GELVIGTKLSASLNGNALTATKLQTPRLVSGVEFDGSKDITLTAAHVAAFAR"
BASE COUNT          167 a          123 c          160 g          190 t
ORIGIN      
        1 tctggcaata tcaaaacaat gaacgagcca tccactcaat aatgatgagt aataagggcg
       61 atgatttgcg ctctgtgttc tatgttgatg gcgctgcttt ccctgttttt gcgtttatcg
      121 aagatggcct gtcaatatcc gcacctggtg ctgatctcgt tgttaatgat acgacctata
      181 agtttggggc aacaaatcca gcgactgaat gtatcgcggc ggacgttatc cttgatttta
      241 agagtgggcg tggtttttat gagtctcatt cgttaatcgt taacgataac ttgtcgtgca
      301 aaaaactttt tgccacagac gaaattgtag cgcgtggtgg taatcagatt cgaatgatag
      361 gtggggagta tggtgcatta tggcgtaatg atggcgctaa aacttacctg ctgcttacca
      421 atcaaggtga tgtttatggt ggctggaata cattaagacc gtttgctatt gataacgcaa
      481 ccggcgaact ggttattgga accaaactgt ccgcaagtct gaacggtaat gcattaacag
      541 caacaaagct gcaaacgcca agactggttt ctggtgttga gtttgatggt tccaaagata
      601 ttactttaac cgccgcgcat gtggctgctt ttgccagaag
//
LOCUS       DAJOWB010000444          640 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.454, whole genome shotgun sequence.
ACCESSION   DAJOWB010000444 DAJOWB010000000
VERSION     DAJOWB010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.454"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..>640)
                     /locus_tag="OHO85_005206"
     CDS             complement(<1..>640)
                     /locus_tag="OHO85_005206"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674388.1"
                     /translation="EEINIDVLRNFFFSKIKEQDSVYTDLPDTYKREHIETDIKIDEN
                     ISTFCNIKTLESINLFFNPKAICNKDKNENIETSINLINKIKNKITSNMHIELSIPSV
                     DYLITDFTIDLEYSINAKKYSKHALMRNQTIDYELISDSISYAKTSTDIDSSHYNNHI
                     AEYQNELYFNSLISSIYASSTLTPEIKIRICNNDLFSLVSDIGKNIRNSNISK"
BASE COUNT          193 a           72 c          103 g          272 t
ORIGIN      
        1 tttttgatat attactattt ctaatgtttt tacctatgtc tgaaaccagg ctaaaaagat
       61 cattattaca tattcttatt tttatttcag gagttaaagt cgatgaagca taaatggatg
      121 atattagact gttgaaatat aactcattct gatattcagc aatatgattg ttataatgtg
      181 aactatcaat gtctgttgat gttttagcat atgagattga gtcggaaatc agctcgtaat
      241 ctatggtttg gtttctcatt aatgcatgct ttgagtattt ctttgcattt atagaatact
      301 ctagatcaat ggtaaagtca gtgattaaat agtctacact tggtatcgat aattctatgt
      361 gcatgttgct tgttatttta ttcttaattt tatttataag attgattgat gtttcaatat
      421 tttcattttt atctttatta caaatggctt tagggttaaa aaagagattg attgattcta
      481 atgtttttat attgcaaaag gtagaaatat tctcgtctat tttaatatcg gtttctatgt
      541 gttctcgttt ataagtatca ggtaggtcgg tataaacaga atcttgttct ttaattttgg
      601 agaagaaaaa gtttctcagt acatcaatat taatttcttc
//
LOCUS       DAJOWB010000445          631 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.455, whole genome shotgun sequence.
ACCESSION   DAJOWB010000445 DAJOWB010000000
VERSION     DAJOWB010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.455"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..453
                     /locus_tag="OHO85_005207"
     CDS             <1..453
                     /locus_tag="OHO85_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="RepA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCQ2674389.1"
                     /translation="YDPLIGCYIPTDITFTPALFAALDVSEDAVVAARRSRVEWENRQ
                     RKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIKRARARRDANRERQDIVTLV
                     KRQLTREISEGRFTANREAVKREVERRVKERMILSRNRNYSRLATASP"
BASE COUNT          155 a          169 c          172 g          135 t
ORIGIN      
        1 tatgatccgc ttatcgggtg ctacattccg accgatatca cgttcacacc ggcgttattt
       61 gccgcccttg atgtgtctga ggatgcagtg gttgctgcgc gccgcagtcg tgttgaatgg
      121 gaaaacagac agcgcaaaaa gcaggggctg gataccctgg gtatggatga actgatagcg
      181 aaagcctggc gttttgtgcg tgagcgtttc cgcagttacc agacagagct taagtcccgt
      241 ggaataaagc gtgcccgtgc gcgtcgtgat gcgaacaggg aacgtcagga tatcgtcacc
      301 ctggtgaaac ggcagctgac gcgtgaaatc tcggaagggc gcttcactgc caatcgtgag
      361 gcggtaaaac gcgaagtgga gcgtcgtgtg aaagagcgca tgattctgtc acgtaaccgt
      421 aattacagcc ggctggccac agcttccccc tgaaagtgac ctcttctgaa taatccggcc
      481 cgcaccggag gcattcgcac gcctgaagcc tgccagcgaa caaaaaagca gcacctcata
      541 caaaaaacaa cctcatcatc caccttctgg tgcatctggc ccccctgttt ttgatacaaa
      601 acacgcctca cagacgggga attttgctta t
//
LOCUS       DAJOWB010000446          625 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.456, whole genome shotgun sequence.
ACCESSION   DAJOWB010000446 DAJOWB010000000
VERSION     DAJOWB010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.456"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            87..518
                     /locus_tag="OHO85_005208"
                     /pseudo
     CDS             87..518
                     /locus_tag="OHO85_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493820.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase NikB"
BASE COUNT          147 a          151 c          172 g          155 t
ORIGIN      
        1 ttcgggggaa aaacgagtat ttttagtcat tctgtttacc tctttctcag ggagtttagt
       61 ctccaggatt cccggggcgg ttcagtaata ccggtgatct cgaggcccgt ttgcagaaga
      121 acgggagtgt tcgtttccgt gaccgtcgga cgggagagtt tgtctgtacg gattatggtg
      181 acagggttgt tttccgtaat caccatgacc gcaatgcgct ggcagataaa ctggatttga
      241 ttgcacctgt attgtttgag cgtgatccca gaatgggctt tgaaccagaa ggaaacgaca
      301 aacaatttaa ccaggtcttt gctgaaatgg ttgcctggca taatgtgaca ggccgtaccg
      361 gtcacgaaga ctacaggatt acacgaccgg atgtggatca tcaccgggaa ggaagtgaac
      421 gctactaccg cgattatatc gcggcgaaca gcaacgatga cgcatcactg cctccgccgg
      481 agcaggataa acgctgggaa ccgccatctc ccgtatgacc tgtgtccctt agcgaaaatc
      541 tcgtagcttc tctctcaatg agggcgggta tttgacatcc tccacgccct aaggacgtgg
      601 gttcctgctt caacgggcgg catcc
//
LOCUS       DAJOWB010000447          622 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.457, whole genome shotgun sequence.
ACCESSION   DAJOWB010000447 DAJOWB010000000
VERSION     DAJOWB010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.457"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>622
                     /locus_tag="OHO85_005209"
     CDS             <1..>622
                     /locus_tag="OHO85_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCQ2674390.1"
                     /translation="ADLPTSQFWSYATYFRVLSFEYLSESISTLLYLDADVVCKGSLK
                     PLTEIIFKDEFAAVIPDNDSTQEACAKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTP
                     YLLKLLRGETKYGSLKYLDQDALNIAFNMNNIYLGKDFDTIYTLKNELHDRSHRKFQQ
                     TITDKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEA"
BASE COUNT          193 a          119 c          124 g          186 t
ORIGIN      
        1 ttgctgatct gccgacgtca cagttttggt cgtacgcgac atacttcagg gtattgtctt
       61 ttgaatatct gagtgaaagt atttccacac tgctgtatct ggatgccgat gttgtttgta
      121 aaggaagcct gaaacctctc acagaaatta tatttaaaga tgagtttgct gcggtcattc
      181 ctgacaatga tagtacgcag gaggcatgtg caaaacgcct caacattccc gaaatgaatg
      241 gacgttattt caatgcaggc gttatctatg tcaatcttaa aaaatggcat gaagcaaatt
      301 tgacaccgta tttactcaag cttttacgag gggaaactaa atatggctct cttaaatatt
      361 tagatcagga tgcgttgaat atcgcattta atatgaataa tatctacctc gggaaggatt
      421 ttgatactat ttataccctg aaaaatgaac ttcatgatcg tagtcatcga aagtttcagc
      481 aaaccattac cgataaaaca gtattgattc actatacagg gataactaaa ccatggcata
      541 gctgggctgg atatccgtct gcatcatact ttaatatcgc gcgtgaacaa tctccctgga
      601 agaaatatcc tcttaaagag gc
//
LOCUS       DAJOWB010000448          619 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.458, whole genome shotgun sequence.
ACCESSION   DAJOWB010000448 DAJOWB010000000
VERSION     DAJOWB010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.458"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..590)
                     /locus_tag="OHO85_005210"
     CDS             complement(<1..590)
                     /locus_tag="OHO85_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCQ2674391.1"
                     /translation="MSRIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMQVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT          146 a          144 c          131 g          198 t
ORIGIN      
        1 tatatgtcag gtgtctgaag ttttgctttg gctcttaaaa tggtttgccg cgaggttttg
       61 aattcccggg caatggcact tatacttaca cctgacttaa ttcgttcgaa taccacctgt
      121 ttctgttctt catttaacac aggtggtcga ccaaaacgtt tccctgcgcc gcgggctctt
      181 actatcccgg aatgagtgcg ttcaagtaaa aggtctcgtt caaattcagc gactgctgaa
      241 attacttgca tcatcatttt tcctgttgga ctggtcaggt caatgccccc caatgctaag
      301 caatgcactc tgatacctgt ttcggtcagt tgttccactg ttttcctgat atccattgca
      361 ttacaaccaa ggcgatccag ttttgtcaca atcaattgat caccacattt caggcgagca
      421 agcaaccggt taaaaccagg acgctcactg gttgctgctg agccgctaat gtgttcttcg
      481 attatttgct gaggtttgat tttaaaacct gcactttcga tttcccggcg ttgattttcg
      541 gtggtctgat ccagcgttga tatccgacag taagcaaaaa ttcgagacat agtgagattc
      601 tatacgaaat tggtgttca
//
LOCUS       DAJOWB010000449          608 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.459, whole genome shotgun sequence.
ACCESSION   DAJOWB010000449 DAJOWB010000000
VERSION     DAJOWB010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.459"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..442
                     /gene="traX"
                     /locus_tag="OHO85_005211"
     CDS             <1..442
                     /gene="traX"
                     /locus_tag="OHO85_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TraX"
                     /protein_id="HCQ2674392.1"
                     /translation="LEQQKRRLDRFRQVNNKKAQLHLTWEEALQASHMSVDDLDRRFR
                     RRRTVWRFCCWSLLAIALFLSGMLFAASSLPLTTLVRAISTLVLILSGVALCASRALI
                     VTYRLWQLHERKVSEPEQGTFRDFLNDRNGWRNATLIAVTSKQY"
BASE COUNT          139 a          167 c          142 g          160 t
ORIGIN      
        1 gctggaacaa caaaaacgca gactggacag atttcgtcag gtaaataata aaaaggcgca
       61 actccacctc acgtgggaag aagctctgca ggccagccat atgtcagtag acgatctgga
      121 tcgtcgtttc cgccggcgca gaacagtatg gcgattctgt tgctggagtc tgctggccat
      181 cgctttgttt ctcagtggta tgctctttgc cgcctcttca ttacctctta cgactctggt
      241 acgcgccatc agcactcttg tgctgatact gtcaggcgtc gctctttgtg cttccagagc
      301 actgattgtt acataccgcc tctggcagtt gcatgaacga aaagtcagcg aacccgagca
      361 ggggactttc cgtgattttc tgaacgaccg caatggctgg cgcaatgcca cgttaattgc
      421 cgtaaccagt aaacaatact gatcacgtcc agatgccagt accactggca tgtcgccatc
      481 ccccttaatt aaatcctgtg tggcctttgc ccatacgcaa ttctattccg gagttactgt
      541 gaaaatttta cttcgcgcac tctgtgcagg ccttgctata tcctcactgc cggcaatggc
      601 ttcagtga
//
LOCUS       DAJOWB010000450          599 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.460, whole genome shotgun sequence.
ACCESSION   DAJOWB010000450 DAJOWB010000000
VERSION     DAJOWB010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.460"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>599
                     /gene="istA"
                     /locus_tag="OHO85_005212"
     CDS             <1..>599
                     /gene="istA"
                     /locus_tag="OHO85_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCQ2674393.1"
                     /translation="FCRCYREWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGE
                     IRRVAIFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVK
                     KADRYEPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETF
                     HTLRALNARLRELLTDMNNRPMKGYGNQTRAERFRILDA"
BASE COUNT          151 a          169 c          157 g          122 t
ORIGIN      
        1 acttctgccg ttgctaccgg gagtggaaaa aaacgcgacg tctctccatg cgccaggagc
       61 atcgtgccgg cgaaaagctt tttatcgact tctgtggtcc tacggttcct gttattaatc
      121 cggacaccgg tgagatacgc cgcgtggcaa tcttcgtggc cgtaatgggg gcgtccaact
      181 atacctacgt tgaagcgtgc gaaggtcagg acatgatgtc atggttaaat gcccacagcc
      241 gttgcctgac cttcctgggc ggcgtaccga agctgctcat ccccgataac ctcagaagcg
      301 cagtgaaaaa ggccgatcga tatgagcctg tcatcaacga cagttatcag gcactggctg
      361 agcactatgg cacggttatc atcccggccc gaccacgcaa acctaaagat aaaccaaaag
      421 cagaaaacgg cgtcctcatc gtcgaacgat ggcttcttgc ccgcatccgt aatgaaacct
      481 tccacacgct cagggcgctg aatgcgcgcc ttagggagtt actgacagac atgaataacc
      541 ggccaatgaa ggggtacggc aatcaaaccc gtgcagaacg cttccgcata ctggatgcg
//
LOCUS       DAJOWB010000451          599 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.461, whole genome shotgun sequence.
ACCESSION   DAJOWB010000451 DAJOWB010000000
VERSION     DAJOWB010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.461"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..108
                     /locus_tag="OHO85_005213"
     CDS             <1..108
                     /locus_tag="OHO85_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transport protein periplasmic
                     component"
                     /protein_id="HCQ2674394.1"
                     /translation="FEKREAVVTFDDTKASVQKLTKATADAGYPSSVKQ"
     gene            144..566
                     /gene="merC"
                     /locus_tag="OHO85_005214"
     CDS             144..566
                     /gene="merC"
                     /locus_tag="OHO85_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial transporter MerC"
                     /protein_id="HCQ2674395.1"
                     /translation="MGLMTRIADKTGALGSVVSAMGCAACFPALASFGAAIGLGFLSQ
                     YEGLFISRLLPLFAALAFLANALGWFSHRQWLRSLLGMIGPAIVFAATVWLLGNWWTA
                     NLMYVGLALMIGVSIWDFVSPAHRRCGPDGCELPAKRL"
BASE COUNT           99 a          194 c          195 g          111 t
ORIGIN      
        1 ttcgagaagc gcgaggccgt cgtcactttt gacgacacca aggccagcgt acagaagctg
       61 accaaggcca ccgcagacgc cggctatccg tccagcgtca agcagtgagc cagcaagcca
      121 acgacaacag cgagagccgc ttcatgggac tgatgacacg cattgccgat aaaaccggcg
      181 cgctcggcag cgtcgtttcc gcgatgggct gcgccgcctg ctttccagcc ctcgccagct
      241 tcggcgcggc catcgggctg ggcttcttga gccagtacga gggactgttc atcagccgcc
      301 tgctgccgct gtttgccgcg ctggccttcc tggcgaacgc gctgggttgg ttcagtcatc
      361 ggcaatggct gcgcagtctg ctcggcatga tcggcccggc catcgtgttt gcggccacgg
      421 tctggctgct cggcaactgg tggacggcga acctgatgta cgtcggcctg gccttgatga
      481 ttggggtgtc gatctgggac ttcgtgtcgc cggcgcatcg ccgttgcgga ccggacggct
      541 gcgaactccc cgccaagcgc ttgtgaaaga cggctgaccg tgcgacacgg cggcccaca
//
LOCUS       DAJOWB010000452          594 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.462, whole genome shotgun sequence.
ACCESSION   DAJOWB010000452 DAJOWB010000000
VERSION     DAJOWB010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.462"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            114..>594
                     /locus_tag="OHO85_005215"
     CDS             114..>594
                     /locus_tag="OHO85_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V secretion protein CvaA"
                     /protein_id="HCQ2674396.1"
                     /translation="MKWQGRAILLPGIPLWLIMLGSIVFITAFLMFIIVGTYSRRVNV
                     SGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKSTRNGIVTDNHRR
                     DIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQRAEEGIKIMKNN
                     "
BASE COUNT          197 a          102 c          130 g          165 t
ORIGIN      
        1 catataatgc aaattttcga tttattttcc atcattaatc cagataaaca acaaactaat
       61 agtatgcaag gagacattat ttgtttcgcc aggatgcttt agaaaacaga aaaatgaagt
      121 ggcagggacg ggcaatatta cttcccggaa taccactgtg gttaatcatg ctgggaagca
      181 ttgtgtttat tacggcattt ctgatgttca ttattgttgg tacctatagc cgccgtgtta
      241 atgtcagtgg tgaggtcaca acctggccaa gagctgtcaa tatatattca ggtgtacagg
      301 gatttgttgt caggcagttt gttcatgaag ggcagttgat aaaaaaaggg gatcctgttt
      361 atctgattga catcagtaaa agtacacgca atggtattgt cactgataat catcgccggg
      421 atatagaaaa ccagctggtt cgtgtggaca acattatttc ccgtctggaa gaaagtaaaa
      481 aaataacgct agatacccta gaaaaacaac gtctgcaata cacagatgcg ttccgtcgct
      541 catcagacat tatacagcgt gcagaggaag ggataaaaat aatgaaaaat aata
//
LOCUS       DAJOWB010000453          583 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.463, whole genome shotgun sequence.
ACCESSION   DAJOWB010000453 DAJOWB010000000
VERSION     DAJOWB010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.463"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>583
                     /locus_tag="OHO85_005216"
     CDS             <1..>583
                     /locus_tag="OHO85_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HCQ2674397.1"
                     /translation="VFCTGVAEKYALIEQWRQQFPIEAMCQVFGVSRSGYYNWVQHEP
                     SDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCK
                     QKRKFRATTNSNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVY
                     TCEIVGYAMGERMTKELTGKALFMALRSQRPPAG"
BASE COUNT          162 a          142 c          162 g          117 t
ORIGIN      
        1 gcgtattttg cacaggagtc gctgaaaaat acgcgttaat cgaacaatgg cgacaacaat
       61 ttcccattga agcgatgtgt caggtatttg gtgtatccag gagcggttat tacaactggg
      121 tacagcatga accctcagac agaaaacaaa gtgatgagcg gctaaaactg gagattaagg
      181 tggcacatat ccgcactcgc gaaacatatg gaacccggcg gctccagacg gagctggcag
      241 agaatggcat catcgttggt cgtgaccgac tggcacgtct tcgtaaggag ctaaggctac
      301 gctgtaagca gaaacgcaag ttcagagcga ctacgaactc gaaccacaat ctgccagttg
      361 cgccaaatct gctgaaccag acgttcgctc ctacagcacc aaatcaggtc tgggtggcgg
      421 acctgacgta tgttgccaca caggagggat ggttgtacct cgctggcatc aaagatgttt
      481 atacgtgcga aattgtcggc tacgccatgg gagagcgcat gacaaaagag ctgacaggta
      541 aagccctgtt tatggcgctc aggagccagc gcccacctgc cgg
//
LOCUS       DAJOWB010000454          580 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.464, whole genome shotgun sequence.
ACCESSION   DAJOWB010000454 DAJOWB010000000
VERSION     DAJOWB010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.464"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..555)
                     /locus_tag="OHO85_005217"
     CDS             complement(<1..555)
                     /locus_tag="OHO85_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCQ2674398.1"
                     /translation="MIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDGSKDNTSNVL
                     REYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNYLLKMHSRQH
                     ETGGDAIYCGLYGHHIKNGVTYSPINTEFNEGSLLFDFFYKKVRFHIGCLFIRKQLLE
                     ENNLFFDEDLRLGEDLDFIYRLLIT"
BASE COUNT          188 a          108 c           87 g          197 t
ORIGIN      
        1 tgtaattagc agtcgataga taaaatccag atcttctcct agtcgtaaat cttcatcaaa
       61 aaaaagatta ttctcttcca gaagttgttt tcttataaac aagcacccta tatggaatct
      121 aaccttttta taaaaaaagt cgaaaagtaa agatccttca ttaaactctg tatttatagg
      181 tgagtaagta acaccatttt ttatatgatg gccataaagc ccacaataaa tggcatctcc
      241 tcccgtctca tgttgtctgg aatgcatctt cagcagatag ttaggatcta tctcatcatc
      301 atcatccata aaacatataa actgtccttg cgccttgcgg atacctgtat tcctggcaga
      361 cgaaacaccg ccattttctt tattgataat aattaatcga gagtgggtta acccatactc
      421 tctcaaaacg tttgatgtgt tatctttaga accatcatta acaataacaa tttcaaaatt
      481 tgggtaagtt tgtttttcta atttcgttag agtttcagta atgtaatcag atgaattata
      541 cgtcggtatt attatggata ctaaagggta atcattgctc
//
LOCUS       DAJOWB010000455          579 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.465, whole genome shotgun sequence.
ACCESSION   DAJOWB010000455 DAJOWB010000000
VERSION     DAJOWB010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.465"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            35..199
                     /locus_tag="OHO85_005218"
     CDS             35..199
                     /locus_tag="OHO85_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCQ2674399.1"
                     /translation="MASKARIAIAIGFLLLSVLVDFTSTILSVLSDGALVAVAVTLVW
                     PILKTASKDQ"
     gene            310..385
                     /locus_tag="OHO85_005219"
     tRNA            310..385
                     /locus_tag="OHO85_005219"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:343..345,aa:Ile,seq:cat)
BASE COUNT          123 a          129 c          170 g          157 t
ORIGIN      
        1 gtcgtttagg tcttcgtgtg gaagattatc tgtaatggca agcaaggcac gtatcgcaat
       61 cgccattggt tttctcttgc tgtccgtgct ggtggatttc accagcacaa tcctgtcagt
      121 tttatcggac ggggcgttgg tggcagtagc tgtaacattg gtatggccga tattaaaaac
      181 agcttctaag gatcagtgat gggcttctgg gattttgctg acaagcatcc aattgttctc
      241 gttgtcattg ctggcatagt tgtaggcggt attgctggcg tcatagaagc actcaggaaa
      301 cagtaatccg gccctttagc tcagtggtta gagctggcga ctcataatcg cacggtcacc
      361 ggttcaagtc cggtaggggc caccatattt ggttgtaaca cggcgtctgg cacatgcgtc
      421 gttagcggtc tggtgacgtt aaaagggggg aaccttgccc ctagctcagg caacgaacca
      481 ggtagccgga atgtgcaagc caccgtttgt tgtttctcgg gtaaagggat tcaccatcct
      541 ggcgattcgg tgtgacagcc gggaagagtc cggcgcatt
//
LOCUS       DAJOWB010000456          554 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.466, whole genome shotgun sequence.
ACCESSION   DAJOWB010000456 DAJOWB010000000
VERSION     DAJOWB010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.466"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            49..180
                     /locus_tag="OHO85_005220"
     CDS             49..180
                     /locus_tag="OHO85_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
                     /protein_id="HCQ2674400.1"
                     /translation="MIEEGLLLPMNVLLQGQKLTLLDPEMWFLRGNENKDVTIFLIN"
     gene            162..554
                     /locus_tag="OHO85_005221"
                     /pseudo
     CDS             162..554
                     /locus_tag="OHO85_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
BASE COUNT          174 a           89 c          133 g          158 t
ORIGIN      
        1 ttatagcaag tttatggaga atgagaatat ggaataagga gatgaaacat gattgaagaa
       61 gggctgttac ttccaatgaa tgttttgctt caaggacaga agttaaccct actggaccct
      121 gaaatgtggt tcttacgggg aaatgaaaac aaggatgtaa ctatattctt gatcaattag
      181 agatgaagta tcctgatcta ttaaaagaga tattaagata tataaccgtg cagcgcattt
      241 cagaggtaat tcagcgtctg atcctggagc gaatttcagt gcgcaatatg cggttagtta
      301 tggaggcctt agcattatgg agcccacgag agaaagatat aatcacactc gtcgagcatg
      361 tacgaggtgc attaggtcgc tacatttgcc ataagttttc atattctgga gaaattaaag
      421 caattgtgat ttcgcctgaa atagaggaca gaatcagaga tggtgtcagg cctaccgctg
      481 gcggtacttt tcttaatctc gatgcatccg aagctgagat gattctggat aattttaaac
      541 tggcactttc tgga
//
LOCUS       DAJOWB010000457          548 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.467, whole genome shotgun sequence.
ACCESSION   DAJOWB010000457 DAJOWB010000000
VERSION     DAJOWB010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.467"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            58..>548
                     /locus_tag="OHO85_005222"
     CDS             58..>548
                     /locus_tag="OHO85_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674401.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPTGNNLEALAPGIEKLKQ
                     TSIEMVTLLNTLQPGGKCIITGDFQKELAYLQNVILYNVSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQEISTGNININCPLKDIYNEIRRLNVIFSCGTGDIVDLSSLDLCN
                     VDL"
BASE COUNT          189 a           91 c           88 g          180 t
ORIGIN      
        1 ttttcacccg atcattttaa taatgatttt tattgattat ttataaagga taaaataatg
       61 ttccctgttt catcaattgg taatgatatt agtagtgatt tagtccgtcg aaaaatgaat
      121 gaccttcctg aaagcccaac aggaaataac ctcgaagcac tagcaccagg tatagaaaaa
      181 ctaaaacaga cctctattga aatggtcact ttacttaaca cgttacaacc tggtggaaaa
      241 tgcattatca ctggtgattt tcaaaaagaa ttagcgtact tacaaaatgt aattctttat
      301 aatgtctcgt ctcttcgtct ggatttttta ggttataacg cccaaattat tcaacgatcg
      361 gacaatactt gtgaacttac cattaatgaa ccgttaaaaa accaggaaat atccacaggt
      421 aatatcaata ttaattgccc attaaaagat atttacaatg aaatcaggag gttaaacgta
      481 atttttagtt gtgggactgg agatatcgtt gatctatcct ctctggactt atgtaatgtc
      541 gatttaga
//
LOCUS       DAJOWB010000458          546 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.468, whole genome shotgun sequence.
ACCESSION   DAJOWB010000458 DAJOWB010000000
VERSION     DAJOWB010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.468"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..>546)
                     /locus_tag="OHO85_005223"
     CDS             complement(<1..>546)
                     /locus_tag="OHO85_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCQ2674402.1"
                     /translation="LARYTYTAGGELRAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGR
                     PESCYRYDDTGRVTEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKRVVK
                     KEHADGSITRSEYDEAGRLKAQTDAAGRRTEYSLHMASGAVTAVTGPDGRTVRYGYNS
                     QRQVTSVTYPDGLRSSREYDE"
BASE COUNT           78 a          230 c          127 g          111 t
ORIGIN      
        1 tctcatcata ctcccggctg ctgcgaagcc cgtccgggta cgtcaccgac gtcacctgcc
       61 gctggctgtt atagccatac cgtaccgtcc tgccgtccgg ccccgtcacc gccgtcaccg
      121 cacccgacgc catatgcagg ctgtactccg tccgccgccc cgccgcatcc gtctgtgcct
      181 tcagcctccc cgcctcatca tactcgctgc gggtaatgct cccgtccgca tgttccttct
      241 tcaccacccg cttcaggcca ccctcgcctt ccgtgtacag cacctcccgc cggttcaggc
      301 tgtccgtgat gattacccgg ctctctccgt actcaaagcg gtagtccagc ccctccgggt
      361 tgacctgctc cgtcacccga ccggtatcat cataccggta gcagctctcc ggcctccccg
      421 cataatggtg cgccaccatc cgcccggcgt gctccgcatc ataagtaaac ccgcgcacct
      481 gcgttccgct gcggtcatac accgcccgca gttcaccacc ggccgtgtac gtgtagcgcg
      541 ccagcg
//
LOCUS       DAJOWB010000459          537 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.469, whole genome shotgun sequence.
ACCESSION   DAJOWB010000459 DAJOWB010000000
VERSION     DAJOWB010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.469"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>537
                     /locus_tag="OHO85_005224"
     CDS             <1..>537
                     /locus_tag="OHO85_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCQ2674403.1"
                     /translation="KGERVATVDPVSRSVSALNRTIENSRPDFVANAPSVDPIVDAMK
                     RLNLGDVSRVVQEGIAQQEQQAKSTTPKGKKRRRKAIPEDIKAQRTEAAEHAREMFDQ
                     KGGAQKSQNQRDARGRFIGKSGSKAAAEDARAERAEKARRKEDDERLNAESGLLKKLS
                     KVAEGIGNPSETRAVDALG"
BASE COUNT          170 a          116 c          149 g          102 t
ORIGIN      
        1 aaaggggaac gcgttgcaac ggttgaccca gtatctcgca gtgtgtcggc attaaatcga
       61 acaatcgaaa attcccggcc agactttgtg gccaatgcgc catcagtaga ccctattgtt
      121 gacgcaatga aacggcttaa tttaggggac gtttctcgtg tagttcagga gggcattgct
      181 caacaggaac agcaggccaa atcaactaca ccaaagggta aaaaacgacg caggaaggct
      241 ataccagagg atataaaggc acaacggacc gaagcagccg aacacgctcg cgaaatgttc
      301 gatcaaaaag gcggtgcgca aaaaagccaa aaccaacgcg atgcgcgtgg tcgttttatt
      361 ggaaagtcag ggagtaaggc cgcagcggaa gatgcccgtg ctgaacgtgc tgaaaaggcc
      421 aggcgcaaag aggatgatga gcgtctaaat gctgaatcag gtttattaaa aaaactgtca
      481 aaagtagctg aaggcatagg taacccttca gagactcgtg ccgttgatgc gttaggt
//
LOCUS       DAJOWB010000460          535 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.470, whole genome shotgun sequence.
ACCESSION   DAJOWB010000460 DAJOWB010000000
VERSION     DAJOWB010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.470"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>535
                     /locus_tag="OHO85_005225"
     CDS             <1..>535
                     /locus_tag="OHO85_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000440161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCQ2674404.1"
                     /translation="NTGKKKGGLLSKLFSLLGKGAGGVASLLMGRGMLKKAGALAFGA
                     LGAKRLVGMLRGGGKKTLAHEGGDLAARAAGKLGLKAVGKGALRAIPLVGTVAGGIYD
                     AVTGWNDTEAQRRAFGLKSGQDPSFQQKAAYTLANVLDLGGLVSGISSAIGDVLKSLG
                     FEDIGNMLQSFSTESIAQ"
BASE COUNT          132 a          103 c          170 g          130 t
ORIGIN      
        1 aacaccggga aaaagaaggg cggcttgctg tctaaactat ttagcctgtt agggaagggg
       61 gccggtggcg tcgcgtcgtt gttaatgggg cgtggcatgc tgaaaaaagc tggagcactc
      121 gcttttggcg ctctgggggc aaagagactc gtaggaatgc tacgcggtgg gggcaagaag
      181 actctcgccc atgaaggcgg agatttggct gcccgggcag caggtaaact tggattaaag
      241 gcagttggta aaggggcgtt acgcgcaatt cccctagtcg gcacagtggc tggaggtatt
      301 tatgatgcgg taaccggttg gaatgataca gaagcgcaac gtcgagcgtt tgggcttaaa
      361 tcaggacaag atccatcatt ccagcaaaaa gccgcttata cgttagccaa tgttcttgat
      421 ttggggggac tggtatctgg tattagcagt gctattggtg atgttctcaa atcacttgga
      481 tttgaggata tcggcaatat gttgcaatca ttttcgacgg aaagtattgc ccagg
//
LOCUS       DAJOWB010000461          535 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.471, whole genome shotgun sequence.
ACCESSION   DAJOWB010000461 DAJOWB010000000
VERSION     DAJOWB010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.471"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>535
                     /locus_tag="OHO85_005226"
     CDS             <1..>535
                     /locus_tag="OHO85_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCQ2674405.1"
                     /translation="VLPEFGGDTWTQTDVFMTQRATGVATYRNNDFFGLVDGLNFALQ
                     YQGKNDSAAKVNNWKGRDVVESNGDGFGLSATYDYEGFGVGATYAKSDRTDGQVSYAK
                     KDPLNASGKTAEVWATGLKYDANNIYLAATYSETQNMTVFGDDFIANKAKNFEAVAQY
                     QFDFGLRPSIAYLHSRGE"
BASE COUNT          136 a          111 c          132 g          156 t
ORIGIN      
        1 cgtgctgcct gagtttggtg gagatacctg gactcagacc gatgtattta tgacccagcg
       61 tgctactggc gtagccacct accgtaacaa tgatttcttc ggcctggttg atggtctgaa
      121 ctttgctttg cagtatcaag gtaaaaatga tagtgctgca aaagtgaaca actggaaagg
      181 ccgtgacgta gttgaatcta atggtgatgg ctttggtctg tctgctactt acgattatga
      241 aggatttggc gttggtgcaa cctatgcaaa atctgatcgt actgatgggc aggtatccta
      301 tgctaaaaag gatcctctga atgcttctgg taaaactgct gaagtatggg ctactggcct
      361 gaaatatgac gcaaataaca tttacctggc tgcaacttat tctgaaactc agaatatgac
      421 tgtcttcggt gatgacttta tcgcgaacaa ggcgaaaaac tttgaagctg ttgctcaata
      481 ccagtttgat ttcggcctgc gtccgtccat cgcttatctg cactctcgcg gtgaa
//
LOCUS       DAJOWB010000462          528 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.472, whole genome shotgun sequence.
ACCESSION   DAJOWB010000462 DAJOWB010000000
VERSION     DAJOWB010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.472"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>528
                     /locus_tag="OHO85_005227"
     CDS             <1..>528
                     /locus_tag="OHO85_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCQ2674406.1"
                     /translation="HADGSVTQSQFDAVGRLKAQTDAAGRTTEYSPDVVTGLITRITT
                     PDGRASAFYYNHHSQLTSATGPDGLEMRRKYDEYGRLIQETAPDGDITRYRYDNPHSD
                     LPCATDDATGSRKTMTWSRYGQLLTFTDCSGYVTRYDHDRFGQVTAVHREEGLSQYHA
                     YDSRGQLTAVKDTQGH"
BASE COUNT          127 a          155 c          157 g           89 t
ORIGIN      
        1 cacgcggacg gcagcgtcac gcagagtcag tttgatgcgg tgggcaggct taaggcacag
       61 acggatgccg caggcaggac aacagagtac agcccggatg tggtgacggg actcatcacg
      121 cgcatcacca cgccggatgg cagggcatcg gcgttttact ataaccacca cagccagtta
      181 acgtcagcca ccgggcctga cgggctggaa atgcgccgca aatatgatga gtacggacgt
      241 ctgattcagg aaaccgcccc tgacggcgat atcacccgct accgttatga taatccacac
      301 agtgacctcc cctgcgcaac ggacgatgcc accggcagcc ggaaaaccat gacgtggagc
      361 cgttacggtc agttgctgac cttcactgac tgttccggtt atgtaacccg ctatgaccat
      421 gaccgttttg gtcaggtgac ggcggtgcac cgcgaggaag gcctgagcca gtaccacgca
      481 tacgacagcc gtggacagtt aactgcggtg aaagacacgc agggccat
//
LOCUS       DAJOWB010000463          523 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.473, whole genome shotgun sequence.
ACCESSION   DAJOWB010000463 DAJOWB010000000
VERSION     DAJOWB010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.473"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            44..>523
                     /locus_tag="OHO85_005228"
     CDS             44..>523
                     /locus_tag="OHO85_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCQ2674407.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     "
BASE COUNT          153 a          107 c          122 g          141 t
ORIGIN      
        1 ttaaaggcat tatcatattt acgaagagat caggatgatg acgatgaaaa aaagtgtatt
       61 gacggcgttt ataactgtgg tatgtgcaac gtccagcgtt atggcagctg atgataatgc
      121 tatcacggat ggcaaagtaa catttaatgg taaagttatt gctccagctt gtaccctggt
      181 agctgcgacg aaagattcag tagtcacatt gccaaatgtc agcgccacca agttgcaaac
      241 taatggagca gtctctggcg ttaaaactga tgtaccaatt gccttagaag gctgtgatgt
      301 tactgtaact aaaaatgcga cgtttacctt tagtggtacg gctgacggcg tgcaacctac
      361 agcatttgcc aaccaggcta ccaccgatgc agcgacgaat gttgctctac aaatgtatct
      421 gccagatggt tctacatcgg taactcccgg taccgaaacg tcgaatattc agttagcgga
      481 ttctgctgag caaacagtga cctttaaagt tgattatatc gct
//
LOCUS       DAJOWB010000464          522 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.474, whole genome shotgun sequence.
ACCESSION   DAJOWB010000464 DAJOWB010000000
VERSION     DAJOWB010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.474"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          129 a          135 c          158 g          100 t
ORIGIN      
        1 tgacgcggtt cctggcgacc tgaaagcccc tggttagggg tatcggcgcg cctgacctcc
       61 tgaaaccctc tgtaagcgtt tttcgcgcct tccatgccct gacgggcatt taaaacccgt
      121 ttcgtgcgcc tgagagcttc tgagagcgtt tgaagagcta tctggcgact catccccgaa
      181 agaataacag cggaatcacc gataacatgc gagaggtggc accgctcgcc gcagccccag
      241 cgaccgaacg ttagtgagcg agtgggcgag gaagcggaag agaaccggaa accacattga
      301 gcacgtacgc actgatgcgg ggtgtcgggg tgaagccctg accagatggc aaacggaata
      361 tcgtcgcggg tgacggtatt acatttgcac atcctgtccc gatttctgcg gagtttaacg
      421 gtgaacggac ggcaaaaaac gtgcaggagc tgttgaacgt gaaaacctcg aaatctggca
      481 aaaatcacgg ctgacgaggc ttgcagagga ttaagcgagg ct
//
LOCUS       DAJOWB010000465          512 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.475, whole genome shotgun sequence.
ACCESSION   DAJOWB010000465 DAJOWB010000000
VERSION     DAJOWB010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.475"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          153 a           99 c          137 g          123 t
ORIGIN      
        1 tcatcttaat ctcaaaatcg gtgcggagtg gttactgcgt gggcagccaa aacgagagat
       61 cactccgaag agaatgaaaa atattatctg atcggatgtt gcacagcggg gcagagtaaa
      121 gtcggctatg tgggggagaa cagcaaaagt tcggcgctgt taatctgact gcagaaccgg
      181 cttaaagcga catatttttg cgctaaagcc atcgcgctgc ttgtggacaa ctacattatc
      241 cataaaggct aggaataccg cgctggctga aggaaagtcc gaagttcaag ggcgtttatc
      301 aaccggttta cttgtcatgg gggaatcata ttgaacggtt atggcaggca attcgcggca
      361 caataacgcg taatcatcaa tgccgaacaa tgtggcaact attgtaagag attcgtcatt
      421 ttatggaaat cgtcagcgca ttccctgaga caaacatggg gtggcaaaag tctagtggca
      481 ttaggctcaa ctatttaggg gaggaataat ta
//
LOCUS       DAJOWB010000466          500 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.476, whole genome shotgun sequence.
ACCESSION   DAJOWB010000466 DAJOWB010000000
VERSION     DAJOWB010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.476"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          121 a          120 c          127 g          132 t
ORIGIN      
        1 ctgaacgcat tgctgaacat tcttgaatct cagttcaggc tttctgtcgt gccggaagga
       61 aaaaccatgt atgttctgct tccccagata gccagtttaa agttgtctga tgtctctggt
      121 atttttacct cgggttatca ctacaataag ctgagagcga agtaaccggt gagcagtttt
      181 tctccattat gcttcagact tagatgattg attattatgg ggatttcatc accgatattc
      241 agatgtatga atatcggtga tgaactaaag ctcaggagct gcgtggtggt gatgaatcaa
      301 caggcatata actgataccc gccggtcaga agttccagcc tgcgactttc ctgtctgcgt
      361 gcccgcagtc cggcgttcag gtgcagcgtg tgacagaacg cacggagagc gaaacggcag
      421 gcagacaggc cgttcagtca gccctggcac cctgcccgga taccagcttc cctgaaacaa
      481 ctggcatcag gtgcgcttat
//
LOCUS       DAJOWB010000467          498 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.477, whole genome shotgun sequence.
ACCESSION   DAJOWB010000467 DAJOWB010000000
VERSION     DAJOWB010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.477"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          158 a          101 c          103 g          136 t
ORIGIN      
        1 tcgtagccgc gtatttaatg gtcttaaaat ataaaacaat tggttactat ctatggcaag
       61 ctcctgaatt atgtgtgtga accaggagtt tctcaggaat ttcctcacaa tgtggcacta
      121 tcactgtcac ctttttctcc ttggaaacct ccagggatgc cccaaagttt cggtcagttt
      181 ttagataggt agcattcctc attaataatg aggacaccga gggtagaatc ttaactccgt
      241 gttaatagaa taaaaaacaa tcatcagaac tgagtaggga aagcgaatca tgatgtatga
      301 agtttcacac acaatgtgaa tcaaaaacgc tcgtagataa tccgatagtg ctgcctgata
      361 cgctgaaaga aattgttcgc aggaagatct gattatgctg attcgtcgac cacaggccct
      421 tatatacata agcagcgccc gctaggtcag aaacaatgtg aacactatcc gtgagaaatt
      481 gtgacaaaat tcccgatg
//
LOCUS       DAJOWB010000468          497 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.478, whole genome shotgun sequence.
ACCESSION   DAJOWB010000468 DAJOWB010000000
VERSION     DAJOWB010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.478"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            3..>497
                     /locus_tag="OHO85_005229"
     CDS             3..>497
                     /locus_tag="OHO85_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="norphogenetic protein"
                     /protein_id="HCQ2674408.1"
                     /translation="MNIPPRATRTVFCVGSGPSLTREDCAAIEKTGCSIIAVNNSWLM
                     FDDIYALYAGDLSWWKQYGSTIPGGRFRKVTANLAAAKSFSLEYRRYCGPAEGVNSGA
                     QAISLAAESGAEVVVLVGYDCSLQNGLHWHGAHPQALRNPTQVSISKWQQQFLDTRKK
                     HADLH"
BASE COUNT          123 a          116 c          138 g          120 t
ORIGIN      
        1 gaatgaacat accacccagg gcgactagga ctgttttttg tgtcggtagc ggtccgtcac
       61 tcactcgtga ggactgtgct gctatagaaa aaactggctg ttcaatcatt gcggttaaca
      121 attcctggct gatgttcgat gacatttatg ccttatacgc cggtgatttg tcatggtgga
      181 agcaatacgg ttccaccata ccgggaggga ggttccgcaa agtgacagcc aacctggcgg
      241 cggcgaaatc attttcgttg gagtacaggc gatattgtgg accggcggaa ggggtaaata
      301 gcggcgcgca ggctatcagt ctggctgctg aatcaggggc tgaagtagtg gtattagtcg
      361 gctatgactg ttctctgcaa aacggccttc attggcatgg cgcgcaccct caagccctac
      421 ggaatccaac gcaggtgtct atttcaaaat ggcaacagca gttcctggat acccgcaaaa
      481 aacacgcaga tttacat
//
LOCUS       DAJOWB010000469          483 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.479, whole genome shotgun sequence.
ACCESSION   DAJOWB010000469 DAJOWB010000000
VERSION     DAJOWB010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.479"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            172..>483
                     /locus_tag="OHO85_005230"
     CDS             172..>483
                     /locus_tag="OHO85_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2674409.1"
                     /translation="MIFSPQHKTDDLMNKKTKRTFTPEFRLECAQLIVDKGYSYRQAS
                     EAMNVGSTTLESWVRQLRRERQGITPSATPITPDQQRIRELEKQVRRLEEQNTILKKA
                     TV"
BASE COUNT          161 a          120 c          102 g          100 t
ORIGIN      
        1 cacactcaac taataatatc tcagcagata ttatcctgac caggacacct cacattactg
       61 aattctcttc cgacagtatc actgcacaaa taaaaaagaa cgggataaat attcagaccg
      121 gaagaatggt gtcactgtag tggcacacta aatttggcca cctgatgaaa ggtgatattc
      181 tcaccacaac acaaaacaga tgacttaatg aacaagaaaa ccaaacgaac cttcacccct
      241 gagttcaggc tggaatgtgc acagctgatt gttgataagg gctactcata tcgacaggcc
      301 agtgaagcga tgaatgtcgg ttcaaccacg cttgagagct gggtacgcca gctcaggcga
      361 gagcgccagg gtattacgcc ctctgccaca cccattactc cagaccagca acgtatccgc
      421 gagctggaaa agcaagttcg ccgtctggag gaacaaaata cgatattaaa aaaggctact
      481 gta
//
LOCUS       DAJOWB010000470          479 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.480, whole genome shotgun sequence.
ACCESSION   DAJOWB010000470 DAJOWB010000000
VERSION     DAJOWB010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.480"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..130)
                     /locus_tag="OHO85_005231"
     CDS             complement(<1..130)
                     /locus_tag="OHO85_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase"
                     /protein_id="HCQ2674410.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGI"
     gene            complement(176..445)
                     /locus_tag="OHO85_005232"
     CDS             complement(176..445)
                     /locus_tag="OHO85_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCQ2674411.1"
                     /translation="MQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFKQLNQLV
                     ESNEKELKDCNLPGKRDKRRAKILASKNYIIWRMVRLVNRKKNKR"
BASE COUNT          158 a           86 c           59 g          176 t
ORIGIN      
        1 taattccagc tgcatgaaga aactttgttg caacatcatc ccataaagac aaagtcatta
       61 aagaaaaaat acataatatg agaattaaag actttatacc agaattaatt tcattccaac
      121 aaaacgacat atagtttcaa cctatgctac gaggatgggt tatttaatgc aagatctaac
      181 gtttattctt ttttctattt accagtctta ccatcctcca gataatataa ttttttgatg
      241 ctaatatttt tgctcgtctc ttatctctct tgccaggaag attacaatct tttaattctt
      301 tttcattgct ttcaactaat tgattcaatt gtttaaaatc accattaagt agaacattcc
      361 acaaataacg agttttatga taagctctat tttgactcaa taatgtatga atttcttctt
      421 tccggttacc tttgtataac tgcatcacag aagagtagat tttttcgtgc gcaaatgac
//
LOCUS       DAJOWB010000471          478 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.481, whole genome shotgun sequence.
ACCESSION   DAJOWB010000471 DAJOWB010000000
VERSION     DAJOWB010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.481"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          150 a          103 c           64 g          161 t
ORIGIN      
        1 ccagaaataa ttatattgtc ttcaaatacg ccagtactat tgattgttat caatacctaa
       61 tcagtacaat cttttcctcc atttcttctt aacatatttc actctatgaa agataaaact
      121 gatacccacc tacagttttg ggtcaccaac aaaaaacaat gttcgccaga tattccacca
      181 ctggcacagt tcaaccctgc ttcaaaaatt gacttattca ttgtgctcat tttcgtagtc
      241 cgttcaggtt gaataaaact cccttcgtca aatgaggaaa gactgagagg ttattacttt
      301 agcttacgtt atagctgttt tcctttgcag tatttctttg cttttctgta tacctttata
      361 cctgttatac cagatcaaaa aacaagcaat ccacataaca aaacgcgttt tgttactgat
      421 gtcacaaatt gagcatattt ttgtagctga tagtttgtta caaacaccag aaccaaga
//
LOCUS       DAJOWB010000472          471 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.482, whole genome shotgun sequence.
ACCESSION   DAJOWB010000472 DAJOWB010000000
VERSION     DAJOWB010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.482"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            152..>471
                     /locus_tag="OHO85_005233"
     CDS             152..>471
                     /locus_tag="OHO85_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674412.1"
                     /translation="MTFTQMANVRDKYTTSISDDMVQLVDWINSQPEKYRERLYRGAM
                     IGRMLIEYQDMKAAGHSAEQIEQQRLSLVSRLQAEIDRFGNPGRGPIAKLSGSGARAW
                     FAFRG"
BASE COUNT          115 a          107 c          142 g          107 t
ORIGIN      
        1 cctgattaat ggcgtatggc aaaaatatgc tggtggtcgc tggattgaag ctgatgcagg
       61 gaaggaactt aagatcgatg ctgccagtta tggcgcggat agctgggagg ctcttcagcg
      121 taacctgact acaacagaag gccgtctcgg catgacattt acccagatgg caaatgtccg
      181 cgataagtac accacatcaa tcagcgacga tatggtgcag ctggtggact ggattaacag
      241 ccagcctgaa aaataccgtg aacgcttgta tcgcggggcg atgattggcc ggatgttaat
      301 tgaatatcag gacatgaagg ccgccgggca tagtgctgaa caaatcgaac agcagcgcct
      361 ttctctggta tcccgtttgc aggcagagat tgaccgtttt ggtaaccccg gtcgcggtcc
      421 gatagcgaaa ttatcgggga gcggtgcgcg cgcctggttt gctttccgtg g
//
LOCUS       DAJOWB010000473          468 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.483, whole genome shotgun sequence.
ACCESSION   DAJOWB010000473 DAJOWB010000000
VERSION     DAJOWB010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.483"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..>468)
                     /locus_tag="OHO85_005234"
     CDS             complement(<1..>468)
                     /locus_tag="OHO85_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCQ2674413.1"
                     /translation="AALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVL
                     PAPLPPYRVLTGLVDRFGRTQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAE
                     EARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPL"
BASE COUNT           77 a          162 c          150 g           79 t
ORIGIN      
        1 agcggcgcgg caggtaaatt ctccgggtat tccgggtcgt gcgtcagcca cacggcagac
       61 agacggatgc cgttgtcccg gccatattcg gtgtaacccg gcagggtatc aggaaaagcg
      121 gacggttccg tcccgccgga aatggcctgc tgccgggctt cttctgcccg ctgcgcctgc
      181 gtggtcagta ccagccggaa gtgacgcccg gcaccatccg tcacgccggt gatttcgccg
      241 ctgaattcac cggcggcttc gcggtggaac gtctgtgtgc gcccgaagcg gtccaccagc
      301 ccggtcagta cccggtacgg cggcagcggc gcaggcagca cctcatccgc ttccggcacc
      361 cgctcacacc agccgagcag ccaccacggc ccctgcggac tgtttgtcgc cagataacga
      421 tgcggactta agcggagttc ttccggcagc gcctgccaga gtgcggcc
//
LOCUS       DAJOWB010000474          461 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.484, whole genome shotgun sequence.
ACCESSION   DAJOWB010000474 DAJOWB010000000
VERSION     DAJOWB010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.484"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..>461)
                     /locus_tag="OHO85_005235"
     CDS             complement(<1..>461)
                     /locus_tag="OHO85_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HCQ2674414.1"
                     /translation="ATNPDDLSRQALVTIFGDVVTNPLSTSAPTLIGSMFGAFNSIIA
                     VLAVVWFMFIGIRHVVRSGHQGQVFSTGRDIVGTLSVVAGFLMIVPTGNGWSIAQLIM
                     LWGASIMGVGSANVMVQLAADNIANGYSMTVQPVQASTQTAARGIFEMELC"
BASE COUNT          123 a          125 c          114 g           99 t
ORIGIN      
        1 acacagttcc atttcaaaaa tgccacgcgc tgctgtttgt gtggatgcct gaactggctg
       61 tacagtcatt gaataaccat ttgcgatatt atcagcggca agttgcacca tcacattggc
      121 ggacccaacc cccattatcg atgcgcccca taacataatg agctgagcga tactccagcc
      181 atttccggtt ggaacgatca tcagaaaacc tgcaacgaca ctcagtgtgc cgacgatatc
      241 gcgtccggtg ctgaatacct gtccctgatg ccccgaacgc acaacatgac ggataccaat
      301 gaacataaac cagacaacgg caagtacggc gatgatactg ttgaacgccc cgaacatgct
      361 tccgatcaga gtgggggcgc tggttgacag tggattggtg actacatcac cgaatatcgt
      421 gaccagcgcc tggcgagaca gatcgtcagg attggttgcg g
//
LOCUS       DAJOWB010000475          454 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.485, whole genome shotgun sequence.
ACCESSION   DAJOWB010000475 DAJOWB010000000
VERSION     DAJOWB010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.485"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..159)
                     /locus_tag="OHO85_005236"
     CDS             complement(<1..159)
                     /locus_tag="OHO85_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCQ2674415.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNTPINNPVLRSITNEMIVLQ
                     YNLSVEHFN"
     gene            complement(175..>454)
                     /locus_tag="OHO85_005237"
     CDS             complement(175..>454)
                     /locus_tag="OHO85_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="required for invasion of epithelial cells; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HCQ2674416.1"
                     /translation="EKDINPEIKINDIKRFIVNSSASVQYENISVVLSKRRDIIEQAP
                     TYEISEPVFAYDKAMPVSILLALISVATCWLLWKYRAILTNLVRLKIK"
BASE COUNT          147 a           77 c           71 g          159 t
ORIGIN      
        1 attaaaatgt tcaactgaaa gattgtactg cagaacaatc atttcattgg taatagatct
       61 aagcaccgga ttgttaatag gtgtattatt caagcttact cgttgaggat gaatataact
      121 aattggatca tatataatct ttcttagggc aagattcata acgctctcat atgtctactt
      181 aattttcaac ctgactaaat tagttagaat ggccctatac ttccataaca gccagcaagt
      241 cgctacggat attaatgcaa gtaagataga aaccggcata gccttatcat aagcaaaaac
      301 aggttcgcta atttcatatg ttggtgcttg ctcaataatg tctcttcgtt ttgacaatac
      361 aacagaaata ttttcatatt gtacgcttgc agagctatta acaataaatc tcttgatatc
      421 atttattttt atttctgggt tgatatcttt ttca
//
LOCUS       DAJOWB010000476          450 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.486, whole genome shotgun sequence.
ACCESSION   DAJOWB010000476 DAJOWB010000000
VERSION     DAJOWB010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.486"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT           56 a          162 c          140 g           92 t
ORIGIN      
        1 ggtatggcct gccaggccgc cggaaaaccc tggcaggctt acgcctgcct gaaatgcagg
       61 agggccgcgc ctgcggcgct gcccttcctg cccgtcatgg tgtgaggcat ttttctgtta
      121 tgtcgtttct gttgttctga tttccggagg cggggacacc ttcggctccc ggctgcctgg
      181 cggcatccgg tcgtcaggcc cgactccgac aggcagctgc tggcgcaccc gccagtctcc
      241 gccgaacccg ctggcgcggt ttcgggctgc cgctacggtc cgatgtctga aaaaccaggc
      301 ttgcagcgac cgttccggtc gcgccgcccc tgtcgcgccg gaaacggcgc tccctgcaca
      361 cctgacggtg tgtaaggcca ctgcgcctga tggcgcagtc aacccttccg ccgccacccc
      421 tgcggcctgt ttcccgctgt ggactttccg
//
LOCUS       DAJOWB010000477          446 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.487, whole genome shotgun sequence.
ACCESSION   DAJOWB010000477 DAJOWB010000000
VERSION     DAJOWB010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.487"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            227..>446
                     /locus_tag="OHO85_005238"
     CDS             227..>446
                     /locus_tag="OHO85_005238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674417.1"
                     /translation="MGKITFVVEFEDGKEPPVSANLDVAGGRLVSVLFGDYRDDFFQP
                     EEVDVVREALNELSVDNDDAHAEIIQKME"
BASE COUNT          118 a           81 c          131 g          116 t
ORIGIN      
        1 ttaatttatt caaagcacgg cgagtatatg gcaggccagg tgcaagggga atacgtggag
       61 ttgagcgacg gcactttatc gtggttaggg aacgccttgt tctggatgcc gctaccagaa
      121 ccgccgcagg aggtgaatca atgagctggc ctgaagcatt caccacggta ggaattgtga
      181 tggcggcagc actgggtttg tattcaattt gtcgctggtg gtaacgatgg gaaaaataac
      241 ttttgtagtc gaatttgagg atggcaaaga gccacctgtt agcgccaatc ttgatgttgc
      301 tggtggcagg cttgtttcgg ttctatttgg tgactaccga gatgacttct tccaaccaga
      361 agaagttgat gtagtgcgag aggcattaaa cgagttaagt gttgataacg atgatgctca
      421 tgcggaaatc atccaaaaaa tggaac
//
LOCUS       DAJOWB010000478          435 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.488, whole genome shotgun sequence.
ACCESSION   DAJOWB010000478 DAJOWB010000000
VERSION     DAJOWB010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.488"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..351
                     /locus_tag="OHO85_005239"
     CDS             <1..351
                     /locus_tag="OHO85_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCQ2674418.1"
                     /translation="AYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIPDR
                     VVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIYQS
                     VAAELYAHDLERLC"
BASE COUNT          129 a          103 c           89 g          114 t
ORIGIN      
        1 gcttatgaac tccaggcact tcgccatatt tttgccatga ctattgatga atgcgcgacc
       61 tggattgccc agaccggtga cagtgaaagc tggcgtcagt gggaaaatgg caaatgcgcc
      121 attcctgatc gtgtagtcga gcaactgttg gctatgcgtc agcaacgaaa aaaacatctt
      181 catgccatca tagagaaaat aaataaccgc atcggcaaca acaccatgcg ttttttcccc
      241 gacttaaccg cgtttcaaca agtctaccct gatggcaatt ttattgactg gaagatctat
      301 caatcggtag ccgctgaatt atatgcacac gatctggagc ggctttgtta atttctccac
      361 aaaaaggaat tgtcgttgtt acaacaataa tgaacggata ctgacacaac atcgctacac
      421 tttttaaagc acctt
//
LOCUS       DAJOWB010000479          434 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.489, whole genome shotgun sequence.
ACCESSION   DAJOWB010000479 DAJOWB010000000
VERSION     DAJOWB010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.489"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(25..336)
                     /locus_tag="OHO85_005240"
     CDS             complement(25..336)
                     /locus_tag="OHO85_005240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674419.1"
                     /translation="MEIVIYITISILILDIALIKQRMLANIETSNPYHAQYILRRRDD
                     YLVLFDRDALSCRFFYDSFPGMRLRHQVTGDTDDDRLAHSPADRMYTGKDHQKYTFCT
                     K"
BASE COUNT          112 a           96 c          109 g          117 t
ORIGIN      
        1 caatttttaa acataaaatg tgaattattt agtacaaaaa gtgtacttct gatgatcttt
       61 ccctgtatac atccggtctg cggggctgtg tgccaggcgg tcatcgtcgg tatcgccggt
      121 cacctggtgg cgcagacgca ttcccggaaa ggagtcataa aagaagcggc agctcagggc
      181 atcacggtcg aacagcacca ggtaatcatc gcggcggcgc aggatgtact gggcgtgata
      241 cgggttactg gtttcgatat tggctaacat ccgctgtttt attaatgcga tatctaaaat
      301 caggatggaa atagtgatgt atatcactat ttccatgatc ttgccttgcg ttcatccagg
      361 caggaatatc cctgttagca gcctctgtgc gatgtacgcc agcggtctgt aaagaaaccg
      421 attaccttat aaca
//
LOCUS       DAJOWB010000480          433 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.490, whole genome shotgun sequence.
ACCESSION   DAJOWB010000480 DAJOWB010000000
VERSION     DAJOWB010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.490"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
BASE COUNT          134 a           67 c           89 g          143 t
ORIGIN      
        1 ctaaaacagc aattgataaa taaacatgtt gatttattca agaaaatgga tgtattttaa
       61 atattgattc ttaatataac ctaatcaata gattatcacg tgagagctgc acattattgg
      121 cggggccggg gaaaacatca ttttgggaac aatgaatcag gtactcttac cactgaagca
      181 agatcatccg ggcacctggt ggtggtgcag tgctttggaa atggtcccct cgggggaatt
      241 gtcattattt tctatgctat ttgcttatga tattcatcag gtgaatttaa taaattccta
      301 atttgaaata tccactatta agccagtgtt taacgacgtt aagatggcaa tgtgcagatc
      361 atgttatcag ggtggggcgg tagcgtatat tccttataca aatcatcatt cattttaatg
      421 attgttattg aat
//
LOCUS       DAJOWB010000481          431 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.491, whole genome shotgun sequence.
ACCESSION   DAJOWB010000481 DAJOWB010000000
VERSION     DAJOWB010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.491"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            50..>431
                     /locus_tag="OHO85_005241"
     CDS             50..>431
                     /locus_tag="OHO85_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HCQ2674420.1"
                     /translation="MGSYFMLRMLDKIKPGGFMCVIVPPSIVSGSNMKRLRLRLSRKA
                     EFLGAHRLPTGTFDANGTSTVVDVVLMRKHPAEMAEKIPLVDESTLESANVLWSTFIS
                     GKWFEKDGRRFVHGTQEKGFQGRIE"
BASE COUNT          104 a          102 c          121 g          104 t
ORIGIN      
        1 tcgtgataac acacgcaaca tcgataagcc ttacgcagaa gaaacggaca tgggttctta
       61 cttcatgctc cgcatgctgg acaagataaa gccaggcgga ttcatgtgcg tgattgtgcc
      121 gccgtccatt gtttcaggtt caaacatgaa gcggttacgc ctgcgcctat cacggaaagc
      181 tgaatttctt ggtgcccacc gcttgcctac cggtactttt gacgctaacg ggaccagtac
      241 ggtcgtagat gtggtgctga tgcgcaaaca tccggcagag atggctgaga aaatccccct
      301 ggtggatgaa agcactctcg aatcggcaaa tgtgctttgg tcaacgttta tttctggcaa
      361 gtggtttgaa aaggatggcc gccggtttgt tcatggcacc caggaaaagg gcttccaggg
      421 gcgtattgag g
//
LOCUS       DAJOWB010000482          428 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.492, whole genome shotgun sequence.
ACCESSION   DAJOWB010000482 DAJOWB010000000
VERSION     DAJOWB010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.492"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..428)
                     /locus_tag="OHO85_005242"
     CDS             complement(<1..428)
                     /locus_tag="OHO85_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCQ2674421.1"
                     /translation="MHVKDALEKVPGVQSADVSYAKGSAKLAIEVGTSPDALMAAVAG
                     LGYRATLADAPSVSTPGGLLDKMRDLLGRNDKTGSSGALHIAVIGSGGAAMAAALKAV
                     EQGARVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRR"
BASE COUNT           69 a          145 c          137 g           77 t
ORIGIN      
        1 tcccggcgca gatgggcgat atgggcggcg cggatcatga tcttggacgg cacacaaccg
       61 acattgacgc aggtgccgcc gatggtgccg cgctcgatca gcgtgacacg tgcgccttgc
      121 tcgacggcct tcagcgccgc tgccatcgcg gccccgccgc tgccgatgac ggcgatatgc
      181 aatgcgccgc tgctacccgt cttgtcgttt ctgcccagca gatcgcgcat cttgtcgagc
      241 aatccgcccg gcgtcgaaac tgagggggca tcggccagcg tggcccgata accgagtcca
      301 gctacagcgg ccatcagcgc gtcgggtgac gtgccgacct caatggcgag cttggcgctg
      361 cccttggcgt aggagacatc cgctgattgc acgccgggca ctttctccag ggcgtccttg
      421 acatgcac
//
LOCUS       DAJOWB010000483          427 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.493, whole genome shotgun sequence.
ACCESSION   DAJOWB010000483 DAJOWB010000000
VERSION     DAJOWB010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.493"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..371
                     /locus_tag="OHO85_005243"
     CDS             <1..371
                     /locus_tag="OHO85_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2674422.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLK
                     GKLTPALLQTLIREIKGSSH"
BASE COUNT          107 a          120 c          111 g           89 t
ORIGIN      
        1 ggcccaactt cccttatgag tttaaaatcg ccttagtgga gcagtcactg cagcccggag
       61 cctgtgtggc gcagatcgcc cgggaaaacg gaatcaacga taacctgctc ttcaactggc
      121 gccatcaata ccggaaaggt ggcctgctgc cttccggaaa aaatatgccg gcactgcttc
      181 ccgtgacgtt aacgccggag ccggataata aaatcccggc ccccgcacag gaaccagagc
      241 agataaatac accgtccgac agtctgtgtt gtgagctggt tctgccggcc ggaactctca
      301 ggcttaaagg taaactgacg ccggcgttat tacagacact tatccgcgaa ataaaaggga
      361 gcagccactg atgatatctc tccctgcagg ttcgcgtatc tggctggttg caggtatcac
      421 cgatatg
//
LOCUS       DAJOWB010000484          424 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.494, whole genome shotgun sequence.
ACCESSION   DAJOWB010000484 DAJOWB010000000
VERSION     DAJOWB010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.494"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>424
                     /locus_tag="OHO85_005244"
     CDS             <1..>424
                     /locus_tag="OHO85_005244"
                     /inference="COORDINATES: protein motif:HMM:NF012626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HCQ2674423.1"
                     /translation="KKHYSRVAGFEANGGFILGSELLFNGTKIDALPTRDALLPVIVI
                     LFAASKKNSYISEMVSELPRRFTLSDRVKDIPNEHSLNILNLLSKDPSNFFRNVGFTN
                     YNVAKVNELDGLRIIFDNNDIIHLRASGNAPELRCYVES"
BASE COUNT          135 a           56 c           86 g          147 t
ORIGIN      
        1 aaaaaaacat tattctcgag tagctgggtt tgaagctaat ggtggtttta ttcttggtag
       61 cgaattgttg tttaatggaa caaaaataga tgctttacca acaagagatg ctcttttgcc
      121 tgtcatcgtg attttatttg cagcatctaa aaagaatagt tatatatcgg aaatggtttc
      181 agaattaccc aggcgtttta cattgtccga cagagtaaag gatataccga atgaacacag
      241 tttaaatatt ttgaatctct tatctaaaga tccctcgaat tttttcagga atgttggctt
      301 tacaaattat aatgttgcaa aagttaacga gcttgatgga ttgaggatca tatttgataa
      361 taatgatatt attcatttgc gtgcatctgg aaatgcacct gagttaagat gttatgtgga
      421 gagt
//
LOCUS       DAJOWB010000485          421 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.495, whole genome shotgun sequence.
ACCESSION   DAJOWB010000485 DAJOWB010000000
VERSION     DAJOWB010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.495"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            44..262
                     /locus_tag="OHO85_005245"
     CDS             44..262
                     /locus_tag="OHO85_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCQ2674424.1"
                     /translation="MGNGTVPSSGAHTHSVSGTAASAGAHAHTVGIGAHTHSVAIGSH
                     GHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            262..>421
                     /locus_tag="OHO85_005246"
     CDS             262..>421
                     /locus_tag="OHO85_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCQ2674425.1"
                     /translation="MAFRMSEQSRTVKIYNLLAGTNEFIGEDDAYIPPHTGLPANSTD
                     IAPPEIPTG"
BASE COUNT          115 a           97 c           98 g          111 t
ORIGIN      
        1 tatgaatttc gactggcttg atggttcatc aagtggcacg gtcgtgggga atggaacagt
       61 gccttcttct ggtgcacaca cccactcagt atcaggcact gctgcaagtg caggggcgca
      121 tgcacataca gttggtattg gcgctcatac gcactctgtt gcgattggtt cacatggaca
      181 taccatcacc gttaatgctg ctggtaacgc ggaaaacacc gttaaaaaca tcgcatttaa
      241 ttatattgtg aggcttgcat aatggcattc agaatgagtg aacaatcacg tacagtaaaa
      301 atttataacc tgctggccgg aactaatgag tttattggtg aagatgacgc atatattcca
      361 cctcatacag ggctgccagc taattctaca gatatcgccc caccggaaat tcctactggc
      421 t
//
LOCUS       DAJOWB010000486          419 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.denovo.496, whole genome shotgun sequence.
ACCESSION   DAJOWB010000486 DAJOWB010000000
VERSION     DAJOWB010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.denovo.496"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..>419
                     /gene="tuf"
                     /locus_tag="OHO85_005247"
     CDS             <1..>419
                     /gene="tuf"
                     /locus_tag="OHO85_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCQ2674426.1"
                     /translation="APEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGA
                     AQMDGAILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEV
                     RELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILEL"
BASE COUNT           99 a          114 c          119 g           87 t
ORIGIN      
        1 gcgccggaag aaaaagctcg tggtatcacc atcaacactt ctcacgttga atacgacacc
       61 ccgacccgtc actacgcaca cgtagactgc ccggggcacg ccgactatgt taaaaacatg
      121 atcaccggtg ctgctcagat ggacggcgcg atcctggtag ttgctgcgac tgacggcccg
      181 atgccgcaga ctcgtgagca catcctgctg ggtcgtcagg taggcgttcc gtacatcatc
      241 gtgttcctga acaaatgcga catggttgat gacgaagagc tgctggaact ggttgaaatg
      301 gaagttcgtg aacttctgtc tcagtacgac ttcccgggcg acgacactcc gatcgttcgt
      361 ggttctgctc tgaaagcgct ggaaggcgac gcagagtggg aagcgaaaat cctggaact
//
LOCUS       DAJOWB010000487         5381 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.guided.212, whole genome shotgun sequence.
ACCESSION   DAJOWB010000487 DAJOWB010000000
VERSION     DAJOWB010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.guided.212"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..339)
                     /locus_tag="OHO85_005248"
     CDS             complement(<1..339)
                     /locus_tag="OHO85_005248"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="poxA; regulates pyruvate oxidase poxB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P lysine(34) lysyltransferase"
                     /protein_id="HCQ2674427.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFT"
     gene            664..2472
                     /gene="frdA"
                     /locus_tag="OHO85_005249"
     CDS             664..2472
                     /gene="frdA"
                     /locus_tag="OHO85_005249"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCQ2674428.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            2465..3199
                     /gene="frdB"
                     /locus_tag="OHO85_005250"
     CDS             2465..3199
                     /gene="frdB"
                     /locus_tag="OHO85_005250"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCQ2674429.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            3210..3605
                     /gene="frdC"
                     /locus_tag="OHO85_005251"
     CDS             3210..3605
                     /gene="frdC"
                     /locus_tag="OHO85_005251"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCQ2674430.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3616..3975
                     /gene="frdD"
                     /locus_tag="OHO85_005252"
     CDS             3616..3975
                     /gene="frdD"
                     /locus_tag="OHO85_005252"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCQ2674431.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            4038..5171
                     /locus_tag="OHO85_005253"
     CDS             4038..5171
                     /locus_tag="OHO85_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCQ2674432.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
BASE COUNT         1325 a         1410 c         1432 g         1214 t
ORIGIN      
        1 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
       61 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
      121 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
      181 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
      241 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcgcgtttta ataagttagg
      301 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
      361 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
      421 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
      481 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
      541 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
      601 taactttcag gtacttaccc tgaagtacgt ggctgtggga taaaaacaat ctggaggaat
      661 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
      721 gctgcaattg ctgccgcgca ggcaaatccg aatgcaaaaa tcgcactaat ctcaaaagta
      781 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
      841 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
      901 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
      961 tggggatgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     1021 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     1081 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     1141 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     1201 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     1261 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     1321 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     1381 tccggtatcc tgatgaccga aggctgccgc ggtgaaggcg gtattctggt caacaaaaat
     1441 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     1501 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     1561 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cctgcgtcac
     1621 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     1681 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     1741 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     1801 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     1861 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     1921 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     1981 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     2041 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     2101 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     2161 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     2221 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     2281 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     2341 ggcacgactc gcctggagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     2401 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     2461 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     2521 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     2581 tgcgctgggc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     2641 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     2701 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     2761 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     2821 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     2881 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     2941 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     3001 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     3061 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     3121 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     3181 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     3241 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     3301 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     3361 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     3421 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     3481 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     3541 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     3601 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     3661 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     3721 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     3781 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     3841 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     3901 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     3961 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     4021 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     4081 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     4141 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     4201 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     4261 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     4321 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttaccgcta
     4381 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     4441 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     4501 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     4561 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     4621 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     4681 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     4741 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtacga agcaatatga
     4801 atccccgtga tatcaacgac aaaacacttc agcaagggat acaactggca caatctcgct
     4861 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtaa
     4921 atcctgacag catcattaac ggcagtggca ataaaattgc actggcagca caccctgtaa
     4981 aagcgattac gcccccaact cctgcagtac gcgcatcatg ggtacataaa acaggggcaa
     5041 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atagtgatgc
     5101 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctggcag attctcaacg
     5161 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     5221 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     5281 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     5341 gtaacagtag tgaataattt cgacgcaaaa cgttatctcg g
//
LOCUS       DAJOWB010000488         2636 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.guided.282, whole genome shotgun sequence.
ACCESSION   DAJOWB010000488 DAJOWB010000000
VERSION     DAJOWB010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.guided.282"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            72..971
                     /gene="aac(3)-VIa"
                     /locus_tag="OHO85_005254"
     CDS             72..971
                     /gene="aac(3)-VIa"
                     /locus_tag="OHO85_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(3)-VIa"
                     /protein_id="HCQ2674433.1"
                     /translation="MTDPRKNGDLHEPATAPATPWSKSELVRQLRDLGVRSGDMVMPH
                     VSLRAVGPLADGPQTLVDALIEAVGPTGNILAFVSWRDSPYEQTLGHDAPPAAIAQSW
                     PAFDPDHAPAYPGFGAINEFIRTYPGCRRSAHPDASMAAIGPDAAWLVAPHEMGAAYG
                     PRSPIARFLAHAGKILSIGAGPDAVTALHYAEAVARIEGKRRVTYSMPLLREGKRVWV
                     TTSDWDSNGILDEYAAPDGPDAVERIARDYLARTRVAQGPVGGAQSRLIDAADIVSFG
                     IEWLEARHAAPAAAALKPKQRRD"
     gene            1178..1498
                     /gene="groES"
                     /locus_tag="OHO85_005255"
     CDS             1178..1498
                     /gene="groES"
                     /locus_tag="OHO85_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCQ2674434.1"
                     /translation="MGSPTRKICAAINIKPLYDRVVIKRTEEEKVSAGGIVIPDSATE
                     KPIKGQVVAVGEGKVFDNGNVRAPKVKVGDQVLFGKYAGTEVKLDGTEYLVVKEDDIF
                     AVLG"
     gene            1554..>2636
                     /gene="groL"
                     /locus_tag="OHO85_005256"
     CDS             1554..>2636
                     /gene="groL"
                     /locus_tag="OHO85_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313151.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCQ2674435.1"
                     /translation="MAAKDIRFGEDARARMVRGVNVLANAVKATLGPKGRNVVLEKSF
                     GAPTITKDGVSVAKEIELADKFENMGAQMVKEVASKTSDNAGDGTTTATVLAQALIRE
                     GMKAVAAGMNPMDLKRGIDKAVTSAVEELKKISKPCSTSKEIAQVGSISANSDTDIGE
                     LIAKAMDKVGKEGVITVEEGSGLENELDVVEGMQFDRGYLSPYFINNPQSMQAELEDP
                     FILLHDKKISNVRDLLPILEGVAKAGKPLLIVAEDVEGEALATLVVNTIRGIVKVCAV
                     KAPGFGDRRKAMLEDMAILTGGTVISEEVGLSLEKATINDLGRAKKVQVSKENTTIID
                     GAGDTADIEARIKQIKAQIEETTSDYD"
BASE COUNT          528 a          870 c          832 g          406 t
ORIGIN      
        1 agccaaacta tcaggtcaag tctgctttta ttatttttaa aagccctaca caaattggga
       61 gatatatcat gatgactgat ccccgcaaaa acggcgattt gcacgaaccc gcgacggcac
      121 ccgcgacgcc ctggtccaaa agcgagctgg tccggcaatt gcgcgacctc ggcgtgcgct
      181 caggcgatat ggtgatgccg catgtgtcgt tgcgcgccgt cgggccgctg gcggacggac
      241 cgcagacact tgtcgatgcg ctgatcgagg ccgtcggccc caccgggaat attctcgcct
      301 tcgtctcgtg gcgcgattcg ccctatgaac agacgctggg tcatgatgcg ccgcccgccg
      361 ccatcgccca aagctggcct gcgttcgacc ccgaccatgc gcccgcctac cccggctttg
      421 gcgcgatcaa cgaatttatc cgaacctatc cggggtgtcg gcgcagcgcc catcccgacg
      481 catcgatggc ggcgatcggg cccgatgcgg cgtggctggt ggcgccgcac gagatgggcg
      541 ccgcttatgg cccccgctcg ccgatcgcgc gttttctcgc ccacgcagga aaaatcctgt
      601 cgatcggcgc cgggcccgat gcagtcaccg cgctccatta tgccgaagcg gtggcgcgga
      661 tcgagggcaa gcgccgcgtc acttattcga tgcccttact gcgcgaaggc aagcgcgtct
      721 gggtcaccac gtccgactgg gattcgaacg gcatcctcga cgaatatgcc gcgcccgacg
      781 gccccgacgc ggtcgaacgg atcgcccgcg actatctcgc ccgcaccagg gttgcgcaag
      841 gcccggtcgg cggcgcgcaa tcccggctga tcgacgcggc cgatatcgtt tccttcggca
      901 tcgaatggct cgaggcgcgc cacgccgcgc cagcggcggc agcgctgaag ccgaaacaac
      961 gccgcgactg agtcgtcgat tattccggac atatattcct ccctctcgcc cagccgcata
     1021 gcatcaggcc cacaagcccg ccgcctcttc aacaaggatg tcgggacgtt cttcagtaaa
     1081 gaacagcttg gcaccctcga caaggatgat tgcccgaggc gatcaggcgg cgaatttttg
     1141 cgcgcgcgca cggtcgcggc ggcgcgcgat aaaggcgatg ggtagcccca cgaggaagat
     1201 atgcgcggcg attaacatca agccgctgta cgaccgcgtc gtcatcaagc gcaccgagga
     1261 agagaaggtc tccgccggtg gtatcgtgat cccggattcg gccaccgaga agccgatcaa
     1321 gggccaggtc gtcgccgtgg gcgagggcaa ggtgttcgac aacggcaacg tgcgcgcgcc
     1381 gaaggtcaag gtcggcgacc aggtgctgtt cggcaagtac gccggcaccg aggtcaagct
     1441 cgacggcacc gagtacctgg tggtgaagga agacgacatc ttcgccgtgc tgggctgagc
     1501 ccgccgcttc gccgcatccc aacccgcaat cacagaactg aggtaattcc gcaatggctg
     1561 ccaaggatat tcgtttcggt gaagacgccc gcgcccgcat ggtccgcggc gtgaacgtgc
     1621 tcgccaacgc cgtcaaggcg accctgggcc cgaagggccg caacgtcgtg ctcgagaaga
     1681 gcttcggcgc cccgacgatc accaaggacg gcgtgtccgt cgccaaggag atcgaactgg
     1741 ccgacaagtt cgagaacatg ggcgcgcaga tggtcaagga agtcgcttcc aagacctccg
     1801 acaacgccgg cgacggcacc accaccgcca ccgtgctggc ccaggccctg atccgcgagg
     1861 gcatgaaggc cgtggccgcc ggcatgaacc cgatggacct caagcgcggt atcgacaagg
     1921 ccgtcacctc ggccgtcgag gagctgaaga agatctccaa gccctgctcg accagcaagg
     1981 agatcgccca ggtcggttcg atctcggcca actccgacac cgacatcggc gagctgatcg
     2041 ccaaggccat ggacaaggtc ggcaaggaag gcgtgatcac cgtcgaggag ggctcgggcc
     2101 tggagaacga gctcgacgtc gtcgagggca tgcagttcga ccgcggctac ctgtcgccgt
     2161 acttcatcaa caacccgcag tcgatgcagg ccgagctgga ggatccgttc atcctgctgc
     2221 acgacaagaa gatctcgaac gtccgcgacc tgctgccgat cctcgagggc gtggccaagg
     2281 ccggcaagcc gctgctgatc gtcgccgagg acgtcgaggg cgaggcgctg gccacgctgg
     2341 tggtcaacac catccgcggc atcgtgaagg tctgcgcggt caaggccccg ggcttcggcg
     2401 accgccgcaa ggcgatgctg gaggacatgg ccatcctcac cggtggcacc gtgatctccg
     2461 aggaagtcgg cctctcgctc gagaaggcga ccatcaacga cctcggccgc gcgaagaagg
     2521 tgcaggtctc gaaggagaac accaccatca tcgacggcgc cggcgacacc gcggacatcg
     2581 aagcccgcat caagcagatc aaggcgcaga tcgaggagac cacctcggac tacgac
//
LOCUS       DAJOWB010000489         2508 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.guided.288, whole genome shotgun sequence.
ACCESSION   DAJOWB010000489 DAJOWB010000000
VERSION     DAJOWB010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.guided.288"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            <1..278
                     /locus_tag="OHO85_005257"
     CDS             <1..278
                     /locus_tag="OHO85_005257"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Tat pathway signal sequence protein"
                     /protein_id="HCQ2674436.1"
                     /translation="QAESKYGVAIGKSAEVLNSQQSVAIGGWAGIENSSGSVALGHGS
                     QVSGENNVVSVGAGPEGYGESVKGAPETHSIVNKTFLFFIFNGLFSC"
     gene            519..1334
                     /gene="sul2"
                     /locus_tag="OHO85_005258"
     CDS             519..1334
                     /gene="sul2"
                     /locus_tag="OHO85_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCQ2674437.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1421..1723
                     /locus_tag="OHO85_005259"
     CDS             1421..1723
                     /locus_tag="OHO85_005259"
                     /inference="COORDINATES: protein motif:HMM:NF014877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCQ2674438.1"
                     /translation="MVRKYFGTDGIRGKANEGAMTAETALRVGMAAGRVFRRGDHRHR
                     VVIGKDTRLSGYMLEPALTAGFTSMGMDVFLFGPLPTTYRKNKRPFHPSPTALDRS"
     gene            complement(1899..>2508)
                     /locus_tag="OHO85_005260"
     CDS             complement(1899..>2508)
                     /locus_tag="OHO85_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="HCQ2674439.1"
                     /translation="DGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSEKRLSL
                     TRGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPNSRHRA
                     LVTPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAMKVIAC
                     IEDPIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
BASE COUNT          484 a          725 c          710 g          589 t
ORIGIN      
        1 gtcaggctga gtcaaaatat ggtgttgcca taggtaagag cgctgaggta cttaattcac
       61 agcagagtgt tgctattggg ggatgggctg gaatagaaaa ttcttcaggc tctgtagctc
      121 tgggccatgg ctcacaggtg tcgggagaaa ataacgtggt atctgttggt gccgggcctg
      181 agggatatgg agagagtgtc aaaggagcac cggaaaccca ctctattgta aacaagacat
      241 ttttattttt tatattcaat ggcttatttt cctgctaatt ggtaatacca tgaaaaatac
      301 catgctcaga aaaggcttaa caatattttg aaaaattgcc tactgagcgc tgccgcacag
      361 ctccataggc cgctttcctg gctttgcttc cagatgtatg ctattctgct cctgcagcta
      421 atggatcacc gcaaacaggt tactcgcctg gggattccct ttcgacccga gcatccgtat
      481 gagactcatg ctcgattatt attattatag aagcccccat gaataaatcg ctcatcattt
      541 tcggcatcgt caacataacc tcggacagtt tctccgatgg aggccggtat ctggcgccag
      601 acgcagccat tgcgcaggcg cgtaagctga tggccgaggg ggcagatgtg atcgacctcg
      661 gtccggcatc cagcaatccc gacgccgcgc ctgtttcgtc cgacacagaa atcgcgcgta
      721 tcgcgccggt gctggacgcg ctcaaggcag atggcattcc cgtctcgctc gacagttatc
      781 aacccgcgac gcaagcctat gccttgtcgc gtggtgtggc ctatctcaat gatattcgcg
      841 gttttccaga cgctgcgttc tatccgcaat tggcgaaatc atctgccaaa ctcgtcgtta
      901 tgcattcggt gcaagacggg caggcagatc ggcgcgaggc acccgctggc gacatcatgg
      961 atcacattgc ggcgttcttt gacgcgcgca tcgcggcgct gacgggagcc ggtatcaaac
     1021 gcaaccgcct tgtccttgat cccggcatgg ggttttttct gggggctgct cccgaaacct
     1081 cgctctcggt gctggcgcgg ttcgatgaat tgcggctgcg cttcgatttg ccggtgcttc
     1141 tgtctgtttc gcgcaaatcc tttctgcgcg cgctcacagg ccgtggtccg ggggatgtcg
     1201 gggccgcgac actcgctgca gagcttgccg ccgccgcagg tggagctgac ttcatccgca
     1261 cacacgagcc gcgccccttg cgcgacgggc tggcggtatt ggcggcgctg aaagaaaccg
     1321 caagaattcg ttaactgcac attcgggata tttctctata ttcgcggttc agcaggcatg
     1381 tcccctttga gggcgacccg acgacaggat aatcgacctt atggtgcgca aatatttcgg
     1441 cacagacggt attcgtggca aagccaacga aggcgcgatg acggcggaaa ccgccttgcg
     1501 cgtcggcatg gcggctggcc gtgtctttcg tcgcggtgac caccgccatc gtgtcgtgat
     1561 cggcaaggat acgcgcctgt cgggctatat gcttgaaccc gcgctcacag ccggtttcac
     1621 ctcgatgggc atggacgtat tcctttttgg cccgctgccg acaacgtata ggaagaataa
     1681 acgccctttt cacccaagtc caacagcttt ggaccgcagt tgactctttc gacacccctg
     1741 cgatgcaacc caatccggct gacggggagc cagcaacgct gaaaatttac cctcctcttt
     1801 cccactagcg gctccttttc cgacaaccag cacggcggat ccctgccgcg gcgctgtgaa
     1861 cgcagcattt tgattggtat cgttggcctt caggctcgtc agtcaaacag acccaggagc
     1921 agctcagccg gtggcgcccg gctttcgggt aacgccctgg tcccgctggt ttcggctttg
     1981 tgcttcaggt gatcaaggat ctgcttgatc actatagggt cttcaatgca ggcgatgact
     2041 ttcatggcgc cgccgcagcc gctgcaggtc tcgatgtcga tattgaaaac acgcttgagc
     2101 cgttgcgccc atgtcatcga cgctcgccgt tgtgctggtg ttgccggttc atcagccacc
     2161 ctgaccttgt tgcccctgcc ccgttttgcc ggcgtgacca acgcccggtg ccgactgttg
     2221 ggtgcgaaca ccccgtggaa gcgggttagg ttgactctgg gcttcggtac cagggcggcc
     2281 agccttgcaa tgaaatccaa tggttcgaaa atgacgtgcg tggtgccgtc ccggtacggc
     2341 gtcttgagct ggtagcgcac gttgccgcct cgtgttaacg acagccgctt ctcggatacc
     2401 gccgggcggc tgatgtaccg gcacagccgt tcgagcttct tgcgttcatc ggccctggcc
     2461 gccacgccgg cgtgcaggct ggacccggct accttgccaa tcccgtca
//
LOCUS       DAJOWB010000490         2254 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.guided.295, whole genome shotgun sequence.
ACCESSION   DAJOWB010000490 DAJOWB010000000
VERSION     DAJOWB010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.guided.295"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            330..1145
                     /locus_tag="OHO85_005261"
     CDS             330..1145
                     /locus_tag="OHO85_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCQ2674440.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(1303..>2254)
                     /locus_tag="OHO85_005262"
     CDS             complement(1303..>2254)
                     /locus_tag="OHO85_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCQ2674441.1"
                     /translation="AMVVEAQAALPMARFWGMGTSASSDGQFFVATEQGEAMNLVNAK
                     YGNTPGLKAYSHVSDQYAPFATQVIPATASEAPYILDGLLMNDAGRHIREQFTDTGGF
                     TDHVFAACAILGYRFAPRIRDLPSKRLYAFNPSAAPAHLRALIGGKVNQAMIERNWPD
                     ILRIAATIAAGTVAPSQILRKLASYPRQNELATALREVGRVERTLFMIDWILDAELQR
                     RAQIGLNKGEAHHALKRAISFHRRGEIRDRSAEGQHYRIAGMNLLAAIIIFWNTMKLG
                     EVVANQKRDGKLLSPDLLAHVSPLGWEHINLTGEYRWPKP"
BASE COUNT          511 a          553 c          634 g          556 t
ORIGIN      
        1 ggcgtacgct tatgggtgcc tttccgcagc ttggaacgcg gatggagaag aggagcaacg
       61 cgatctagct atcgcggccg cgatcaagca ggtgcgacag acgtcatact agatatcaag
      121 cgacttctcc tatcccctgg gaacacatca atcttaccgg agaatatcgt tggccaaagc
      181 cttagcgtag gatttcgccc tctcccgcaa acgaccccgg gaaccacgtt gtgtctcaaa
      241 atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg
      301 cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcttgct
      361 cgaggccgcg attaaattcc aacctggatg ctgatttata tgggtataaa tgggctcgcg
      421 ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag
      481 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
      541 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc
      601 ctgatgatgc atggttactc accactgcga tccccgggaa aacagcattc caggtattag
      661 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt
      721 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc
      781 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta
      841 atggctggcc tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg
      901 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
      961 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
     1021 tcctatggaa ctgcctcggt gaattttctc cttcattaca gaaacggctt tttcaaaaat
     1081 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt
     1141 tctgatagtt agtctttgta gagacacaaa cctgaaattc cggacaccgc gcttcaggac
     1201 atcgctcaag acgcctatat cgggcagtat ccggacggta ctatcgaacg ccggtaccct
     1261 gccctttatg gggtcgtttg cgggaggggg cggaatccta cgctaaggct ttggccagcg
     1321 atattctccg gtgagattga tgtgttccca tccgagcggc gaaacatggg ccaagagatc
     1381 gggcgatagc agctttccat cgcgtttctg gtttgcaacg acctcgccga gcttcatggt
     1441 gttccagaag atgatgatgg cggcgagcag attcatgccg gcgatgcggt aatgctggcc
     1501 ttcggcggaa cggtcgcgga tttcaccgcg gcggtggaag ctgattgccc gcttcagcgc
     1561 atgatgagct tcgcctttgt tgagcccgat ctgggcacgc cgttggagtt cggcatccag
     1621 aatccagtcg atcatgaaca gggtgcgctc gacgcgaccg acttcccgca gggctgtcgc
     1681 gagctcgttc tgccgcggat aggaggcgag tttccgcaga atctggcttg gcgcgacggt
     1741 cccggcagca atggtggcgg cgatgcgcag gatgtcgggc caattgcgct cgatcatggc
     1801 ttggttgacc tttccgccga tcaacgctcg caggtgcgcc ggggcggccg acggattgaa
     1861 cgcgtagagc cgtttggatg gcaggtcgcg gatgcgcgga gcgaaccggt agccgagaat
     1921 ggcacatgcg gcaaagacgt gatcggtgaa gccgcccgtg tcggtgaact gctcgcggat
     1981 atggcgtcca gcatcgttca tcagcaggcc atcgaggatg taaggcgctt cgcttgccgt
     2041 tgcaggaatc acctgggttg cgaacggcgc atattggtcg gagacgtggc tataggcttt
     2101 caggcccggg gtattgccat atttcgcgtt gaccaggttc atggcctcac cttgctctgt
     2161 agcgacgaag aactgtccgt cgctcgaagc cgacgtgccc atgccccaga accgggccat
     2221 gggtaacgct gcctgtgcct cgaccaccat ggcc
//
LOCUS       DAJOWB010000491         2193 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.guided.299, whole genome shotgun sequence.
ACCESSION   DAJOWB010000491 DAJOWB010000000
VERSION     DAJOWB010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.guided.299"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            89..892
                     /locus_tag="OHO85_005263"
                     /note="aph(3'')-Ib"
     CDS             89..892
                     /locus_tag="OHO85_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCQ2674442.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            892..1728
                     /locus_tag="OHO85_005264"
     CDS             892..1728
                     /locus_tag="OHO85_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCQ2674443.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            1946..>2193
                     /locus_tag="OHO85_005265"
     CDS             1946..>2193
                     /locus_tag="OHO85_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3') family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCQ2674444.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPT"
BASE COUNT          486 a          565 c          609 g          533 t
ORIGIN      
        1 agcatgttct cggacttacg tagcaactcg tttcttttcg caggttgagc cacctccgcg
       61 cttcatcaga aaactgaagg aacctccatt gaatcgaact aatatttttt ttggtgaatc
      121 gcattctgac tggttgcctg tcagaggcgg agaatctggt gattttgttt ttcgacgtgg
      181 tgacgggcat gccttcgcga aaatcgcacc tgcttcccgc cgcggtgagc tcgctggaga
      241 gcgtgaccgc ctcatttggc tcaaaggtcg aggtgtggct tgccccgagg tcatcaactg
      301 gcaggaggaa caggagggtg catgcttggt gataacggca attccgggag taccggcggc
      361 tgatctgtct ggagcggatt tgctcaaagc gtggccgtca atggggcagc aacttggcgc
      421 tgttcacagc ctatcggttg atcaatgtcc gtttgagcgc aggctgtcgc gaatgttcgg
      481 acgcgccgtt gatgtggtgt cccgcaatgc cgtcaatccc gacttcttac cggacgagga
      541 caagagtacg ccgcagctcg atcttttggc tcgtgtcgaa cgagagctac cggtgcggct
      601 cgaccaagag cgcaccgata tggttgtttg ccatggtgat ccctgcatgc cgaacttcat
      661 ggtggaccct aaaactcttc aatgcacggg tctgatcgac cttgggcggc tcggaacagc
      721 agatcgctat gccgatttgg cactcatgat tgctaacgcc gaagagaact gggcagcgcc
      781 agatgaagca gagcgcgcct tcgctgtcct attcaatgta ttggggatcg aagcccccga
      841 ccgcgaacgc cttgccttct atctgcgatt ggaccctctg acttggggtt gatgttcatg
      901 ccgcctgttt ttcctgctca ttggcacgtt tcgcaacctg ttctcattgc ggacaccttt
      961 tccagcctcg tttggaaagt ttcattgcca gacgggactc ctgcaatcgt caagggattg
     1021 aaacctatag aagacattgc tgatgaactg cgcggggccg actatctggt atggcgcaat
     1081 gggaggggag cagtccggtt gctcggtcgt gagaacaatc tgatgttgct cgaatatgcc
     1141 ggggagcgaa tgctctctca catcgttgcc gagcacggcg actaccaggc gaccgaaatt
     1201 gcagcggaac taatggcgaa gctgtatgcc gcatctgagg aacccctgcc ttctgccctt
     1261 ctcccgatcc gggatcgctt tgcagctttg tttcagcggg cgcgcgatga tcaaaacgca
     1321 ggttgtcaaa ctgactacgt ccacgcggcg attatagccg atcaaatgat gagcaatgcc
     1381 tcggaactgc gtgggctaca tggcgatctg catcatgaaa acatcatgtt ctccagtcgc
     1441 ggctggctgg tgatagatcc cgtcggtctg gtcggtgaag tgggctttgg cgccgccaat
     1501 atgttctacg atccggctga cagagacgac ctttgtctcg atcctagacg cattgcacag
     1561 atggcggacg cattctctcg tgcgctggac gtcgatccgc gtcgcctgct cgaccaggcg
     1621 tacgcttatg ggtgcctttc cgcagcttgg aacgcggatg gagaagagga gcaacgcgat
     1681 ctagctatcg cggccgcgat caagcaggtg cgacagacgt catactagat atcaagcgac
     1741 ttctcctatc ccctgggaac acatcaatct taccggagaa tatcgttggc caaagcctta
     1801 gcgtaggatt tcgccctctc ccgcaaacga ccccgggaac cacgttgtgt ctcaaaatct
     1861 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     1921 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctcgag
     1981 gccgcgatta aattccaacc tggatgctga tttatatggg tataaatggg ctcgcgataa
     2041 tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt
     2101 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     2161 aaactggctg acggaattta tgcctcttcc gac
//
LOCUS       DAJOWB010000492         2013 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.guided.307, whole genome shotgun sequence.
ACCESSION   DAJOWB010000492 DAJOWB010000000
VERSION     DAJOWB010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.guided.307"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..1163)
                     /gene="pepD"
                     /locus_tag="OHO85_005266"
     CDS             complement(<1..1163)
                     /gene="pepD"
                     /locus_tag="OHO85_005266"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HCQ2674445.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQDILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGA"
     gene            1424..1882
                     /gene="gpt"
                     /locus_tag="OHO85_005267"
     CDS             1424..1882
                     /gene="gpt"
                     /locus_tag="OHO85_005267"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCQ2674446.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDNYVVDIPQDTWIEQPWDMGVVFVPPISGR"
BASE COUNT          486 a          523 c          501 g          503 t
ORIGIN      
        1 gcgccagcca gtttacccag cgaatccagc atgctcacca cgtagtcttt accgctgtcg
       61 atcagtgaac ggatcaggca gtgaatttct acgttattgt cagtcatggt caccacaccg
      121 acgttcaggg aggtttcgac cacacctttt gccacgtcgg aattgcggat cacaccgttc
      181 ggggtggcgt tcagcagacg aataaaggta tcgcgagatt tcgcaatcag agcagcttta
      241 tcgttcgcta cagagtccag caacaaggcc agattcttct ctttctctgc cagctcgttt
      301 ttcaggatgt cctgataggt attcaccaga gatttcaggg cgtcgacttt atcagctgcg
      361 acagcaatgg tcgcaaaggc ttcacgcggg atggcgttac gcagtgtgcc accgttgaaa
      421 tcgataaggc gcaggtccag ctcttccgca tgacccgcca ggaagcgcac cagcagtttg
      481 ttggcattac ccaggccaac gtggatttca ccgccggagt gaccgccttt tagacctttt
      541 aaggttaact tgaaggtttc aaagccagct ggaaccgctt cacgatccaa atgcaggttg
      601 gaggtgaagt cgataccccc cgcgcaaccc atgtagattt caccttcttc ttcggagtcg
      661 gtgttaatca gaatatcagc ctgcaaccag ttgctctgta agccaaacgc accgtccata
      721 ccggcttctt cggtcatggt cagcagcact tccagcgggc cgtgaaccac gttttcgtca
      781 gccagaaccg ccagcgcaga cgccatacca atgccgttat ctgcacccag cgtggtgccg
      841 cgcgctttaa cccattcgcc atcaatataa ggctggatag gatctttcgt gaagtcatgc
      901 acggtgtcgt tatttttctg cggcaccata tcgaggtggg cctgcaagac gaccggttta
      961 cgattttcca tacctgcggt agcaggttta cgaatcagga tattacctac ctgatcgcgt
     1021 tcgacatgga aacctttctc ttttgcccaa ccaacaatgt attcagcgag ttgctcttca
     1081 tgataggacg ggtgaggaat agaacagatt ttggcaaaaa tatcccacag cggctgtgga
     1141 gataattgag acagttcaga cacgttaagt ctccttgtcg atcacccgca aaacagtatt
     1201 gcaggtcaca gggttagcag aaaatgttgt caacacaaga caggcttgcg agatatgttt
     1261 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     1321 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     1381 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     1441 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     1501 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctggtaccgg gtgcgttact
     1561 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
     1621 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
     1681 tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa
     1741 agcgcacttt gtcaccatct ttgcaaaacc ggctggtcgt ccgctggttg ataattatgt
     1801 tgttgatatc ccgcaagata cctggatcga acagccgtgg gatatgggcg tcgtattcgt
     1861 cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt
     1921 taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
     1981 aggcaaacct gagcgaaacc ctgttcaaac ccc
//
LOCUS       DAJOWB010000493         1849 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.guided.318, whole genome shotgun sequence.
ACCESSION   DAJOWB010000493 DAJOWB010000000
VERSION     DAJOWB010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.guided.318"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..223)
                     /gene="tetR(B)"
                     /locus_tag="OHO85_005268"
     CDS             complement(<1..223)
                     /gene="tetR(B)"
                     /locus_tag="OHO85_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCQ2674447.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGES"
     gene            305..1510
                     /gene="tet(B)"
                     /locus_tag="OHO85_005269"
     CDS             305..1510
                     /gene="tet(B)"
                     /locus_tag="OHO85_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCQ2674448.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLMSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1623..1811)
                     /locus_tag="OHO85_005270"
                     /pseudo
     CDS             complement(1623..1811)
                     /locus_tag="OHO85_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          459 a          353 c          405 g          632 t
ORIGIN      
        1 agctttcccc ttctaaaggg caaaagtgag tatggtgcct atctaacatc tcaatggcta
       61 aggcgtcgag caaagcccgc ttatttttta catgccaata caatgtaggc tgctctacac
      121 ctagcttctg ggcgagttta cgggttgtta aaccttcgat tccgacctca ttaagcagct
      181 ctaatgcgct gttaatcact ttacttttat ctaatctaga catcattaat tcctaatttt
      241 tgttgacact ctatcattga tagagttatt ttaccactcc ctatcagtga tagagaaaag
      301 tgaaatgaat agttcgacaa agatcgcatt ggtaattacg ttactcgatg ccatggggat
      361 tggccttatc atgccagtct tgccaacgtt attacgtgaa tttattgctt cggaagatat
      421 cgctaaccac tttggcgtat tgcttgcact ttatgcgtta atgcaggtta tctttgctcc
      481 ttggcttgga aaaatgtctg accgatttgg tcggcgccca gtgctgttgt tgtcattaat
      541 aggcgcatcg ctggattact tattgctggc tttttcaagt gcgctttgga tgctgtattt
      601 aggccgtttg ctttcaggga tcacaggagc tactggggct gtcgcggcat cggtcattgc
      661 cgataccacc tcagcttctc aacgcgtgaa gtggttcggt tggttagggg caagttttgg
      721 gcttggttta atagcggggc ctattattgg tggttttgca ggagagattt caccgcatag
      781 tccctttttt atcgctgcgt tgctaaatat tgtcgctttc cttgtggtta tgttttggtt
      841 ccgtgaaacc aaaaatacac gtgataatac agataccgaa gtaggggttg agacgcaatc
      901 gaattcggta tacatcactt tatttaaaac gatgcccatt ttgttgatta tttatttttc
      961 agcgcaattg ataggccaaa ttcccgcaac ggtgtgggtg ctatttaccg aaaatcgttt
     1021 tggatggaat agcatgatgg ttggcttttc attagcgggt cttggtcttt tacactcagt
     1081 attccaagcc tttgtggcag gaagaatagc cactaaatgg ggcgaaaaaa cggcagtact
     1141 gctcggattt attgcagata gtagtgcatt tgccttttta gcgtttatat ctgaaggttg
     1201 gttagttttc cctgttttaa ttttattggc tggtggtggg atcgctttac ctgcattaca
     1261 gggagtgatg tctatccaaa caaagagtca tcagcaaggt gctttacagg gattattgat
     1321 gagccttacc aatgcaaccg gtgttattgg cccattactg tttgctgtta tttataatca
     1381 ttcactacca atttgggatg gctggatttg gattattggt ttagcgtttt actgtattat
     1441 tatcctgcta tcgatgacct tcatgttaac ccctcaagct caggggagta aacaggagac
     1501 aagtgcttag ttatttcgtc accaaatgat gttattccgc gaaatataat gaccctcttg
     1561 ataacccaag agggcatttt ttacgataaa gaagatttag cttcaaataa aacctatcta
     1621 ttttatttat ctttcaagct caataaaaag ccgcggtaaa tagcaataaa ttggcctttt
     1681 ttatcggcaa gctcttttag gtttttcgca tgtattgcga tatgcataaa ccagccattg
     1741 agtaagtttt taagcacatc atcatcataa gctttaagtt ggttctcttg gatcaatttg
     1801 ctgacaatgg cggtaatgac tccaacttat tgatagtgtt ttatgttca
//
LOCUS       DAJOWB010000494         1573 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.guided.333, whole genome shotgun sequence.
ACCESSION   DAJOWB010000494 DAJOWB010000000
VERSION     DAJOWB010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.guided.333"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            6..353
                     /locus_tag="OHO85_005271"
     CDS             6..353
                     /locus_tag="OHO85_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HCQ2674449.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            347..1186
                     /gene="sul1"
                     /locus_tag="OHO85_005272"
     CDS             347..1186
                     /gene="sul1"
                     /locus_tag="OHO85_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HCQ2674450.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            1314..>1573
                     /locus_tag="OHO85_005273"
     CDS             1314..>1573
                     /locus_tag="OHO85_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCQ2674451.1"
                     /translation="MDSEEPPNVRVACSGDIDEVVRLMHDAAAWMSAKGTPAWDVARI
                     DRTFAETFVLRSELLVASCSDGIVGCCTLSAEDPEFWPDALKG"
BASE COUNT          280 a          467 c          478 g          348 t
ORIGIN      
        1 atatcatgaa aggctggctt tttcttgtta tcgcaatagt tggcgaagta atcgcaacat
       61 ccgcattaaa atctagcgag ggctttacta agcttgcccc ttccgccgtt gtcataatcg
      121 gttatggcat cgcattttat tttctttctc tggttctgaa atccatccct gtcggtgttg
      181 cttatgcagt ctggtcggga ctcggcgtcg tcataattac agccattgcc tggttgcttc
      241 atgggcaaaa gcttgatgcg tggggctttg taggtatggg gctcataatt gctgcctttt
      301 tgctcgcccg atccccatcg tggaagtcgc tgcggaggcc gacgccatgg tgacggtgtt
      361 cggcattctg aatctcaccg aggactcctt cttcgatgag agccggcggc tagaccccgc
      421 cggcgctgtc accgcggcga tcgaaatgct gcgagtcgga tcagacgtcg tggatgtcgg
      481 accggccgcc agccatccgg acgcgaggcc tgtatcgccg gccgatgaga tcagacgtat
      541 tgcgccgctc ttagacgccc tgtccgatca gatgcaccgt gtttcaatcg acagcttcca
      601 accggaaacc cagcgctatg cgctcaagcg cggcgtgggc tacctgaacg atatccaagg
      661 atttcctgac cctgcgctct atcccgatat tgctgaggcg gactgcaggc tggtggttat
      721 gcactcagcg cagcgggatg gcatcgccac ccgcaccggt caccttcgac ccgaagacgc
      781 gctcgacgag attgtgcggt tcttcgaggc gcgggtttcc gccttgcgac ggagcggggt
      841 cgctgccgac cggctcatcc tcgatccggg gatgggattt ttcttgagcc ccgcaccgga
      901 aacatcgctg cacgtgctgt cgaaccttca aaagctgaag tcggcgttgg ggcttccgct
      961 attggtctcg gtgtcgcgga aatccttctt gggcgccacc gttggccttc ctgtaaagga
     1021 tctgggtcca gcgagccttg cggcggaact tcacgcgatc ggcaatggcg ctgactacgt
     1081 ccgcacccac gcgcctggag atctgcgaag cgcaatcacc ttctcggaaa ccctcgcgaa
     1141 atttcgcagt cgcgacgcca gagaccgagg gttagatcat gcctagcatt caccttccgg
     1201 ccgcccgcta gcggaccctg gtcaggttcc gcgaaggtgg gcgcagacat gctgggctcg
     1261 tcaggatcaa actgcactat gaggcggcgg ttcataccgc gccaggggag cgaatggaca
     1321 gcgaggagcc tccgaacgtt cgggtcgcct gctcgggtga tatcgacgag gttgtgcggc
     1381 tgatgcacga cgctgcggcg tggatgtccg ccaagggaac gcccgcctgg gacgtcgcgc
     1441 ggatcgaccg gacattcgcg gagaccttcg tcctgagatc cgagctccta gtcgcgagtt
     1501 gcagcgacgg catcgtcggc tgttgcacct tgtcggccga ggatcccgag ttctggcccg
     1561 acgccctcaa ggg
//
LOCUS       DAJOWB010000495         1392 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.guided.345, whole genome shotgun sequence.
ACCESSION   DAJOWB010000495 DAJOWB010000000
VERSION     DAJOWB010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.guided.345"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            26..1225
                     /gene="tet(A)"
                     /locus_tag="OHO85_005274"
     CDS             26..1225
                     /gene="tet(A)"
                     /locus_tag="OHO85_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HCQ2674452.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
BASE COUNT          211 a          433 c          446 g          302 t
ORIGIN      
        1 gcggaggctg gtccggaggc cagacgtgaa acccaacaga cccctgatcg taattctgag
       61 cactgtcgcg ctcgacgctg tcggcatcgg cctgattatg ccggtgctgc cgggcctcct
      121 gcgcgatctg gttcactcga acgacgtcac cgcccactat ggcattctgc tggcgctgta
      181 tgcgttgatg caatttgcct gcgcacctgt gctgggcgcg ctgtcggatc gtttcgggcg
      241 gcggccggtc ttgctcgtct cgctggccgg cgctgctgtc gactacgcca tcatggcgac
      301 ggcgcctttc ctttgggttc tctatatcgg gcggatcgtg gccggcatca ccggggcgac
      361 tggggcggta gccggcgctt atattgccga tatcactgat ggcgatgagc gcgcgcggca
      421 cttcggcttc atgagcgcct gtttcgggtt cgggatggtc gcgggacctg tgctcggtgg
      481 gctgatgggc ggtttctccc cccacgctcc gttcttcgcc gcggcagcct tgaacggcct
      541 caatttcctg acgggctgtt tccttttgcc ggagtcgcac aaaggcgaac gccggccgtt
      601 acgccgggag gctctcaacc cgctcgcttc gttccggtgg gcccggggca tgaccgtcgt
      661 cgccgccctg atggcggtct tcttcatcat gcaacttgtc ggacaggtgc cggccgcgct
      721 ttgggtcatt ttcggcgagg atcgctttca ctgggacgcg accacgatcg gcatttcgct
      781 tgccgcattt ggcattctgc attcactcgc ccaggcaatg atcaccggcc ctgtagccgc
      841 ccggctcggc gaaaggcggg cactcatgct cggaatgatt gccgacggca caggctacat
      901 cctgcttgcc ttcgcgacac ggggatggat ggcgttcccg atcatggtcc tgcttgcttc
      961 gggtggcatc ggaatgccgg cgctgcaagc aatgttgtcc aggcaggtgg atgaggaacg
     1021 tcaggggcag ctgcaaggct cactggcggc gctcaccagc ctgacctcga tcgtcggacc
     1081 cctcctcttc acggcgatct atgcggcttc tataacaacg tggaacgggt gggcatggat
     1141 tgcaggcgct gccctctact tgctctgcct gccggcgctg cgtcgcgggc tttggagcgg
     1201 cgcagggcaa cgagccgatc gctgatcgtg gaaacgatag gcctatgcca tgcgggtcaa
     1261 ggcgacttcc ggcaagctat acgcgcccta ggagtgcggt tggaacgttg gcccagccag
     1321 atactcccga tcacgagcag gacgccgatg atttgaagcg cactcagcgt ctgatccaag
     1381 aacaaccatc ct
//
LOCUS       DAJOWB010000496         1214 bp    DNA     linear   BCT 14-OCT-2022
DEFINITION  TPA_asm: Escherichia coli isolate PP238
            SAMN25142522-rid15609603.guided.367, whole genome shotgun sequence.
ACCESSION   DAJOWB010000496 DAJOWB010000000
VERSION     DAJOWB010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN25142522
            Sequence Read Archive: SRR17681127
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 14-OCT-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001453694.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001453694.1
            Genome Coverage       :: 35x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/14/2022 19:35:50
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 5,276
            CDSs (total)                      :: 5,178
            Genes (coding)                    :: 5,016
            CDSs (with protein)               :: 5,016
            Genes (RNA)                       :: 98
            rRNAs                             :: 2, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 82
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 162
            CDSs (without protein)            :: 162
            Pseudo Genes (ambiguous residues) :: 0 of 162
            Pseudo Genes (frameshifted)       :: 65 of 162
            Pseudo Genes (incomplete)         :: 85 of 162
            Pseudo Genes (internal stop)      :: 40 of 162
            Pseudo Genes (multiple problems)  :: 26 of 162
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN25142522-rid15609603.guided.367"
                     /isolate="PP238"
                     /isolation_source="Commercial Turkey"
                     /db_xref="taxon:562"
                     /country="USA"
                     /lat_lon="37.09 N 95.71 W"
                     /collection_date="2017"
                     /collected_by="Johnson Lab"
     gene            complement(<1..499)
                     /gene="intI1"
                     /locus_tag="OHO85_005275"
     CDS             complement(<1..499)
                     /gene="intI1"
                     /locus_tag="OHO85_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCQ2674453.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTII"
     gene            648..>1214
                     /locus_tag="OHO85_005276"
     CDS             648..>1214
                     /locus_tag="OHO85_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HCQ2674454.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLT"
BASE COUNT          291 a          322 c          343 g          258 t
ORIGIN      
        1 cgatgatcgt gccgtgatcg aaatccagat ccttgacccg cagttgcaaa ccctcactga
       61 tccgcatgcc cgttccatac agaagctggg cgaacaaacg atgctcgcct tccagaaaac
      121 cgaggatgcg aaccacttca tccggggtca gcaccaccgg caagcgccgc gacggccgag
      181 gtcttccgat ctcctgaagc cagggcagat ccgtgcacag caccttgccg tagaagaaca
      241 gcaaggccgc caatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc
      301 aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga
      361 aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca
      421 agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
      481 gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc
      541 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
      601 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg
      661 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
      721 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
      781 acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
      841 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
      901 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
      961 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     1021 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     1081 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     1141 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     1201 atgtagtgct tacg
//
